LOCUS       DASWNE010000001        15860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_353205, whole genome shotgun sequence.
ACCESSION   DASWNE010000001 DASWNE010000000
VERSION     DASWNE010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 15860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15860
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353205"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="VGB68_00005"
     CDS             <1..565
                     /locus_tag="VGB68_00005"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0047637.1"
                     /translation="VASRIKSSVSTMSRMINDLLAYASAQIGREIPILPKSANLENIC
                     RVSLDEIRTIYPDAVFHFESSGNLDGYFDAARIQQVLSNLLNNAVKYGQSGTPVTLAA
                     AGEAEAIILQVKNFGNAIPPESLQVIFNPLIQLPSNDSNSHSYPDASIGLGLFIAREI
                     VKGHNGTIQVKSSEADGTVFIIQLPRN"
     gene            606..1661
                     /gene="waaC"
                     /locus_tag="VGB68_00010"
     CDS             606..1661
                     /gene="waaC"
                     /locus_tag="VGB68_00010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEY0047638.1"
                     /translation="MKILIVKLGSIGDIVHTLPALAAIRRALPHAEISWVVENRSAEI
                     LRPGRNQFLSRLIEVDTKALRHQESLGETLLIARQQLRGLRASAFDIALDFQGLLKSA
                     SIAKLAKARRRYGFAREALREPASRFLLTGSVSVPRRIHVIRKNLALVEKALKIPVPE
                     KDFEFPIFTEKAHVEEAEKIISATGENFAILNPAGGWATKLWHAEKFGALADKLWEEN
                     NLISVIATAPNENALAEKVLENSKSGKAVAANLSLKGLYELTKRARVYVGGDTGPTHL
                     AAAAKAPIVGIFGATEWWRNGSPNEMDICVERADIGCRVDCHRRTCGNWICMDIPVET
                     VFEAVQKRLEKVVSGKR"
     gene            1801..2595
                     /locus_tag="VGB68_00015"
     CDS             1801..2595
                     /locus_tag="VGB68_00015"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEY0047639.1"
                     /translation="MTRKIIHALLFKLTILACCGLSFAQSGGKNEVFTVGESLSYEGK
                     MSKAIFRGISVADINFTVARASNGQDYLIKTEARSKGSLTKLFRFSFLQSFESTVEAE
                     KFRILKTTKRDEQGERVRDSEALFDYAEKRVTYIETDPNDRMRPPRKIASGIDQNTQD
                     LISGIYTLRRLPLAVGKTFHVTVSDSGLVYKIPVRVAAREQQNTILGKVWCFRVEPEV
                     FGTNRVIEQKGTMTIWIMDDPRRVPVRAQINTGGYKIEVKLKKPTL"
     gene            2691..3806
                     /locus_tag="VGB68_00020"
     CDS             2691..3806
                     /locus_tag="VGB68_00020"
                     /inference="COORDINATES: protein motif:HMM:NF027583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HEY0047640.1"
                     /translation="MRISGVLLPERILYIFMNEKEIEKIDLEEQETETLEFSLTDFKL
                     NLVSSAEMLKGETKLLPENELKFATGEEESEKEEPTEVFKELAEPKLPALPSENRARL
                     QMQSPNRIFFYWSLKNNPFQTLQKMFGSRAGDYTFVAKLVNLTNRTENFYPVPAAGSW
                     WFDAESDSTYQAEIGFLSVASPFVRLLFSNTIETPRSAPSPYFDLSPQFSVSAQEFAE
                     VLDVSGYKQDAFEMALAGDEAAEADAATEETLRQLIGKRKLDAPGGELRYVLFALATG
                     TALEDLRGQISPNLFAQMEKLVRENAESLAAEKVLSALQENFDLEADEETEEPGEVSY
                     KTFGASLVNFQKFPRTLTKRFPKFSPVSSSKVAESSR"
     gene            3891..5477
                     /locus_tag="VGB68_00025"
     CDS             3891..5477
                     /locus_tag="VGB68_00025"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein
                     domain-containing protein"
                     /protein_id="HEY0047641.1"
                     /translation="MPVGYFSLILHAHLPFVRHPEYPEFLEEDWFYEAITEVYLPLVF
                     IFQNLHEAGAKPRLAMNVSPPLCEMLADKLLEERYTRHLENLIELANREVGRTRKEAP
                     EFSDAAQMYAETLTASLDLWNNKYQRNLINAFRQLQEEGVLEIITCGATHGFLPLMSR
                     REARRAQIQVAVKNYRKHFGRDPRGIWLPECAYEEGIEDLLKEAGIEYFISDTHAILY
                     GDPRPRYGVHAPVRCPNGVATFARDVETSQQVWSAEVGYPGNALYREFYRDVGWDLDY
                     DYIKPHLHADGNRRNLGLKYYRITGKTEHKQPYHPNAAREKTAQDAADFLDKRIRQAY
                     ELREVYEGQSPLVVSPYDAELYGHWWFEGPQFLDFFFRKLHYDQTEIQAISPGDYLDS
                     GLPIQTQQPSASSWGERGYYKVWLNEGTSWMYPYQHDAEAKMIELANRFSQADALMLR
                     ALNQLARELLLAQSSDWAFQIYQGTTVEYASRRFRSHIHRFNLLATQIEKGEINEELL
                     AEIESRDNIFQEIDYRIYKS"
     gene            complement(5505..6020)
                     /locus_tag="VGB68_00030"
     CDS             complement(5505..6020)
                     /locus_tag="VGB68_00030"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY0047642.1"
                     /translation="MLKKISISLAALLLGFGLTFGAIFFWRNNTPRKPEANLIGGNLP
                     AANLSTANGEIFDNKELSKGKVLIMFLSADCTACKAEIQQIAEMYPTISSQIQIYGIN
                     VDPKEKQNALAENKDINFPVLIDDKREFANSLLVKGVPTKFLIQDGIVKKVFVGRFID
                     RNDARQKMELP"
     gene            complement(6123..6410)
                     /locus_tag="VGB68_00035"
     CDS             complement(6123..6410)
                     /locus_tag="VGB68_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047643.1"
                     /translation="MKSVKMIFFSFFIGAVLATAAAYSANMGPCITCIDYYTDYRTSE
                     LCSLTYADPYAQTCDYSCLPTDLNTPQPEGTYVTYCGFDNPCTVSNPEDCH"
     gene            6629..7948
                     /locus_tag="VGB68_00040"
     CDS             6629..7948
                     /locus_tag="VGB68_00040"
                     /inference="COORDINATES: protein motif:HMM:NF019606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEY0047644.1"
                     /translation="MANKGVEIAPANGKLGILLVGLGAVSTTFVAGVEAIRKGEAKPI
                     GSLTQMGTIRLGKRTENRAPRIKDFVPLADLNDIVFGAWDIFSDSAYEAALHAGVLEK
                     SQIEDLREQLESLKPMQAVFSQNYVKRLNGTNIKGGKNKMELAEQLIEDIARFKQEKG
                     CDRLVIVWAASTEIFLEPSEVHETLEAFEKGLYDDDERIAPSMIYAYAALKSGVPFAN
                     GAPNLTVDIPALTRLAEQNAVPICGKDFKTGQTFMKTILAPGLKARMLGLAGWYSTNI
                     LGNRDGEVLDDPESFKTKEESKLSVLEHILQPEVYPDLYEGFSHVVRINYYPPRGDNK
                     EGWDNIDIFGWLNYPMQIKVNFLCRDSILAAPLVLDLALFLDLAQRAGMKGGVQEWLS
                     FYFKAPQTAPGLYPEHDIFIQLKKLKNTLRHLQGEELITHLGLEYYD"
     gene            8315..9517
                     /locus_tag="VGB68_00045"
     CDS             8315..9517
                     /locus_tag="VGB68_00045"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0047645.1"
                     /translation="MSNETAKKKQATETHILLDPDLKSPRAKEFEDKLMGQIVGQERA
                     VRRMSGLFQIYLAGMNNPSRPIGTLLFLGPTGSGKTRVVEAAAEVLFGDPLSVVKIDC
                     AEFQHSHEIAKLIGSPPGYLGHRETSPMLTQENLDKAHTDETKLTFVLFDEIEKASDA
                     LWQLLLGILDKATLTLGDNRRVDFSKAVVIMTSNLGAREMSEMISGGIGFAPGKGQRI
                     QEENEIDQKIYRTASEAAKRKFSPEFMNRIDKVVVFRSLKEHHLREILKIELKQVQNR
                     ITESAGTKFVFECTDDAKEFLLREGIDLKYGARHLKRAIERFLVYPLSNLVATEQVST
                     GDLVTIDYDGEKLTFAKQSGKMIVNESDNFLFEEEMESKSDAVGLPLPQAQAAASGKG
                     NKGDNQES"
     gene            complement(9782..11266)
                     /gene="glpK"
                     /locus_tag="VGB68_00050"
     CDS             complement(9782..11266)
                     /gene="glpK"
                     /locus_tag="VGB68_00050"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEY0047646.1"
                     /translation="MKYILALDQGTTSSRAIVFDKESRIVSLSQKEYTQIFPRSGWVE
                     HNPQEIWETQSQTAIEALEKANLTAADIEAIGITNQRETTLCWNRKTGEPVYNAIVWQ
                     DRRTSAFCDGIRAKYADLIRAKTGLEVDAYFSASKLNWILETVENARVLAEKGELCFG
                     TIDSWLIWKMTGGELHLTDVSNASRTMLFNIRDLNWDEELLEIFDVPRSILPQVTSSS
                     EVYGEVKTPGKLSGIRIAGNAGDQQAALFGQACLRAGMSKNTYGTGCFMLQNTGTKPV
                     ESKNKLLTTVGWQIDNITEYALEGSVFIGGAVIQWLRDALGIIEKSADVEALAGSVAD
                     NGGVYFVPAFAGLGTPHWNQTARGTIIGLTRGTGKAHIARAALESIAYQTADLLDAMH
                     ADAGIELKELRVDGGATSNTGLLQFQADILQIPVLRSRITETTALGAGYLAGLAVGFW
                     KSKDEISQAWQVDKIFEPQMPAERAKELRARWTKAIERAKDWED"
     gene            complement(11508..12068)
                     /locus_tag="VGB68_00055"
     CDS             complement(11508..12068)
                     /locus_tag="VGB68_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEY0047647.1"
                     /translation="MNATEPQFWMMIASIVIAICFIVMAIALFWIAFIVRRVIGTVSR
                     VEEKIDPILDKIDPLMTKVNAIGEQGRQISVQFTEVSNHLSTASKNFSESTALIKQEV
                     AELKLLVGQTAIVAKDKVELVSKTIDRTHAQVASTTDFIQHKVVEPAREIAAIMAGFR
                     KGLEVLLAPAPKQIDRVYMDDEMFIG"
     gene            complement(12099..12560)
                     /locus_tag="VGB68_00060"
     CDS             complement(12099..12560)
                     /locus_tag="VGB68_00060"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEY0047648.1"
                     /translation="MTNERQEETSATAKLTYLLIGGGIGAIVALLFAPKSGHELRGDI
                     ADVTRKGVEKGKEAATQIQERAGEYYEVTRDRAGELYSAAQEKAGELTERAKGAVAQT
                     ANPFSAALEAGKQAYSDEKRRTDTAAVPERPTYPVESKGLNAPKTGDETEG"
     gene            complement(12757..13608)
                     /gene="prmC"
                     /locus_tag="VGB68_00065"
     CDS             complement(12757..13608)
                     /gene="prmC"
                     /locus_tag="VGB68_00065"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEY0047649.1"
                     /translation="MSNIAENIKIAAQILQESGIAEPRREANSLLAFALRKDKAFLIA
                     RPEYELSGEEEKRFREFLRRRAAREPFQHITGRQEFYGLDFVVTKDVLIPRTETELIV
                     ENACQILRDKENPAFCEVGVGSGCISVSILHEIKTASAIGFDVSEKAVEIAKLNAVNN
                     GVINRLGLKISDVFAALETEKFDLIVSNPPYIPRQDIEALQPEVRNFDPLTALTDGAD
                     GLSIIEKIIRESPFFLKPGGFLLLEIGFNQAAKVREMFRAETWQSVKILPDLQGIPRV
                     VRARLKG"
     gene            complement(13621..14397)
                     /locus_tag="VGB68_00070"
     CDS             complement(13621..14397)
                     /locus_tag="VGB68_00070"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0047650.1"
                     /translation="MNVQEYNSAAWDKEVEKGNDWSVAVSAETIDEARRGNWSIVLTP
                     VKPVPREWFGEVKGKDILCLASGGGQQAPVLAAAGASVTVFDNSAKQLEQDRFVAERD
                     NLNLRIEKGDTADLSRFAESGFDLIFHPCSNCFMPKLEPIWRECFRVLRRGGALLVGF
                     TKPEVFIFDSKAEDEEGVLRVRHSLPYSDLESIGEDERNEILQSGRPLEFSHTLEEQI
                     GGQIAAGFVVTGFYEDYWHDAARLINKYMPAFVGMKSIKP"
     gene            complement(14475..>15860)
                     /locus_tag="VGB68_00075"
     CDS             complement(14475..>15860)
                     /locus_tag="VGB68_00075"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEY0047651.1"
                     /translation="SKEFVPYAGVVFFAVPVRLAMLSYQRVYRLQGAFSYIAEFIKVF
                     KAVAVGSLLIIAFTFLFRGGFAFRDFSYSRGVFLLDFVVALVVFTAFHLALRYAQTIF
                     RASGINLIPTLIVGTNAEAEQTIRELKERPDLGYRVIGIVSANAETRRGGDAEKDIEN
                     WNLKPGTNIIGTIDDLPDLIRELEIQEVIITDDKIPSEKFFEAMMQVGRKQKVEFRFA
                     PNLFNVLPQKTSIEQIGVLPMVRLFREPLSDAERLVKRISDIVISLLAIISSAPIWIV
                     ISILIKLDSRGSVLFRQERVGMDGRIFLCYKFRTMQAGADENLHREAYRKNIEGATEE
                     ANAGDAEKPVFGKVKNDPRRTRAGKFLRRTSLDELPQFLNVLRGEMSVVGARPPIPYE
                     VAEYDLRHRRRLDMKPGITGLWQVSGRNRLTFEEMVKIDLYYIENWSLWLDLKIILLT
                     LPAILRGDGAR"
BASE COUNT         3914 a         4122 c         3955 g         3869 t
ORIGIN      
        1 tgtcgcctcg cgcatcaaaa gctccgtctc gactatgagc cggatgatta acgacctgct
       61 ggcatatgcc agcgcgcaaa tcggaaggga aatacccatt ttaccgaagt cggcaaatct
      121 ggaaaacatc tgccgcgtat cgctcgacga gattcggaca atttacccgg atgccgtttt
      181 tcattttgag agttcgggca atctcgacgg atattttgac gccgcgcgaa ttcaacaggt
      241 tctctcgaat cttctaaata atgcggtaaa gtacggtcag agcgggacgc ctgtgacgct
      301 tgccgcggcg ggcgaagcgg aagcgattat cttacaggta aaaaactttg gaaacgcaat
      361 tccgcccgaa tcattacagg taatcttcaa tcctttaata caattgcctt ccaacgactc
      421 aaattcccac tcttatccgg atgccagtat cgggctcggt cttttcatcg cgcgtgaaat
      481 tgtcaaaggg cataacggga ctattcaggt taagtcatcg gaagcggacg gaaccgtctt
      541 tataatacag cttccccgaa attgactttt aactcgcgac tttttacatt cacactttgc
      601 agcgaatgaa gattcttatc gttaaactcg gcagcatcgg cgacatcgtg cacactctgc
      661 ccgcgctcgc ggcaattcgc cgcgcgctgc cgcacgccga gatttcgtgg gtggtcgaaa
      721 atcgctcggc ggaaatcctg cgccccggac gcaaccaatt tttaagtcgc ctcatcgaag
      781 ttgatacgaa ggcgctgagg catcaagaat cgcttgggga gacgctgttg attgcccgtc
      841 agcagttacg cgggcttcgt gcctccgcct tcgacatcgc cctcgatttc caggggcttc
      901 tgaaatccgc atcaatcgcc aaactcgcca aagcccgacg ccggtacggt ttcgcccgcg
      961 aagctctgcg cgagcccgcg agccgttttc ttctgaccgg cagcgtcagc gttccgcgcc
     1021 gaattcacgt tattcgcaaa aatttggcgc tcgtcgagaa agccttaaaa attcccgtgc
     1081 ccgaaaaaga tttcgagttt ccgattttta ccgaaaaagc gcacgtcgaa gaagccgaaa
     1141 aaattatttc cgcaacgggc gaaaattttg cgattttaaa tccggcgggc ggctgggcga
     1201 caaagctctg gcacgcggaa aaattcggcg ctctggctga taagctctgg gaagaaaaca
     1261 atttaatttc cgtcatcgcg accgcgccca atgaaaacgc gctggcggaa aaagttttag
     1321 aaaacagcaa atcgggcaag gcggtcgcgg caaatttaag cttgaagggc ttgtacgaat
     1381 taacgaaacg agcgcgagtt tacgtcggcg gcgacacggg accgacgcat ctggcggcgg
     1441 cggcaaaagc tccgatcgtc ggcattttcg gtgcgaccga atggtggcgc aacggcagcc
     1501 cgaacgaaat ggacatttgc gtcgagcgag ccgacatcgg ttgtcgtgtc gattgtcatc
     1561 gccggacgtg cggaaactgg atttgtatgg acatcccggt cgagacggtt ttcgaagccg
     1621 tgcagaaacg tctggaaaaa gtggtaagcg gtaaacggta agcggtaagt tcgctgcctt
     1681 gagattgcgg ctttatctat tgcagcttac cgcttaccgt ttcaaaggcg agacgccttt
     1741 gaaaaatatt ttttttaaca caaaatccga ttttcgattt atactggcat agataatttt
     1801 atgacgagaa aaatcattca cgcgcttttg tttaagctga cgattctggc ttgctgcggc
     1861 ttgagctttg cgcagtcggg cggaaaaaac gaagtcttta cggtcggcga gagcttatct
     1921 tacgaaggaa aaatgagcaa agcgattttt cgcggcatca gcgtcgccga tataaatttt
     1981 accgtcgccc gcgcctcgaa cgggcaggat tacctgatta aaaccgaagc caggtcgaaa
     2041 ggaagcctga caaaactgtt tcgcttcagc tttctgcaat cgttcgaatc gaccgtcgaa
     2101 gccgaaaaat tccgcattct gaaaacgacc aagcgcgacg agcagggcga gcgcgtgcgc
     2161 gattcggaag cattattcga ttatgctgaa aagcgcgtga cctacatcga aaccgacccg
     2221 aatgaccgga tgcgaccgcc gcgcaagatt gcctccggca tcgaccaaaa cacgcaggat
     2281 ttgatttccg gcatttacac tctgcggcgc ttgccgctgg cggtcggaaa aacgtttcac
     2341 gtcacggtca gcgattccgg tctggtttat aaaatcccgg tgcgcgtcgc ggcgcgcgag
     2401 cagcaaaaca cgattctcgg caaagtctgg tgttttcgcg tcgagccgga agttttcggc
     2461 acaaatcgcg tcatcgaaca aaaaggcacg atgacgattt ggattatgga cgacccgcgc
     2521 cgcgtcccgg ttcgcgcgca gattaacacc ggcggttata aaatcgaagt gaaacttaaa
     2581 aaacccacgc tctgaaattg gtctggaaag aagagccgga gattcttcat tccagactct
     2641 tcggcttgtt tcttttctca aaagccagca taatcaatct tttaatttaa atgcgcattt
     2701 cgggcgtgct tttgcccgag cgcattttat acatatttat gaacgaaaaa gagattgaaa
     2761 aaattgattt ggaagagcag gaaacggaaa cgctcgaatt ttcgctgact gattttaaac
     2821 tgaatctggt ttcatcggcg gaaatgctga agggcgaaac gaaactgctg ccggaaaacg
     2881 agctgaaatt tgcgaccggc gaagaggaat cggaaaaaga ggagccgacc gaagttttta
     2941 aagagcttgc cgagccgaaa ttgcccgcgc tgccgagcga aaaccgcgcg cgtctgcaaa
     3001 tgcagtcgcc gaaccggatt ttcttttact ggtcgctaaa aaataatccg tttcagacgc
     3061 tgcaaaaaat gttcggcagc cgcgccggag attacacgtt tgtcgccaaa ctcgtcaatc
     3121 tgaccaatcg gacggaaaat ttttatccgg ttcccgccgc cggttcgtgg tggttcgatg
     3181 cggaatcgga ctcgacctat caagccgaaa tcggattttt gtccgtcgcc agcccgtttg
     3241 ttcgccttct gttttccaac accatcgaga cgccgcgcag cgcgccgtcg ccgtatttcg
     3301 atttgtcgcc gcagttttcc gtctcagcgc aggaatttgc cgaggtgctc gacgtttccg
     3361 gttacaaaca ggacgctttt gaaatggctc tcgccggcga cgaagccgca gaagcggacg
     3421 cggcaaccga agaaacgctg cggcagttaa tcggcaagcg aaaactcgac gcgccgggcg
     3481 gcgaattgcg ctatgtttta ttcgcgctcg caacgggcac ggcgctcgaa gatttgcgcg
     3541 gtcaaatcag cccgaatctt ttcgcgcaga tggaaaagct ggtcagggaa aacgccgaaa
     3601 gcctcgcggc ggaaaaagtt ttgtcggctt tgcaggaaaa tttcgacctc gaagccgatg
     3661 aagagaccga agagcccggc gaagtttcct acaaaacttt cggagcgagc ctcgtcaatt
     3721 ttcaaaagtt tccgcgaacg ctgacgaaaa gattcccgaa attttcgccc gtcagctctt
     3781 ctaaagttgc agaaagtagc agatagttac aaagttgcgg aaagtttaaa acttccgccg
     3841 gctgtctata actatctgta actttctgca actatctgct aactttaatt atgcctgttg
     3901 gttatttcag cctaatttta cacgcgcatc tgccgtttgt gcggcatcct gaatatcccg
     3961 aatttctgga agaagactgg ttttacgaag cgattacgga agtttatctg ccgctggttt
     4021 tcatctttca gaacctgcac gaggcgggcg ccaagcctcg tcttgcaatg aacgtttcgc
     4081 cgccgctgtg cgagatgctc gccgataagc ttctggaaga aagatacacg cggcatctgg
     4141 aaaatttaat cgagctggcg aacagggaag tcgggcgaac aaggaaagaa gcgccggaat
     4201 tttcggacgc cgcgcagatg tatgccgaaa ccttaacggc gtcgctcgat ttgtggaaca
     4261 ataaatacca gcgaaatctc atcaacgctt ttcgccagct tcaggaagaa ggcgttctgg
     4321 aaatcatcac ctgcggcgcg acgcacggtt ttctgccctt gatgtcgcgc cgcgaagcgc
     4381 gccgggcgca gattcaggtg gcggtcaaaa attaccggaa acatttcggg cgcgacccgc
     4441 gcggcatctg gctgcccgaa tgcgcgtatg aagaaggcat cgaagattta ctgaaagaag
     4501 ccggaatcga atattttatc tcggacactc acgccattct ttacggcgac ccgcgcccgc
     4561 gctacggcgt gcacgcgccc gtcagatgcc cgaacggcgt ggcgactttc gcgcgcgatg
     4621 tcgagacttc acaacaggtt tggagcgccg aagtcggtta tccgggcaac gcgctctacc
     4681 gcgaatttta ccgcgatgtc ggctgggatt tggattacga ttacatcaaa ccacatttgc
     4741 acgccgacgg aaaccgccgc aatctgggtt taaaatatta ccgcatcacc ggcaaaaccg
     4801 agcacaaaca gccgtatcat ccgaatgcgg cgcgcgaaaa aaccgcgcag gacgccgccg
     4861 attttctcga caaacgcatc cggcaggctt acgagctgcg tgaggtttac gaaggtcagt
     4921 cgcctttagt cgtttcgcct tacgacgccg agctttacgg tcactggtgg tttgaaggtc
     4981 cgcagtttct ggatttcttt ttccgcaaac tgcattacga ccagacggaa attcaggcaa
     5041 tctcgccggg cgattatctg gattccggct tgccgattca gacgcagcag ccttcggcgt
     5101 cgagctgggg cgagcgcggt tactacaaag tctggctcaa cgagggaact tcgtggatgt
     5161 atccgtacca gcacgatgcc gaagcgaaaa tgattgagct ggcgaatcgt ttcagtcagg
     5221 cggacgcact tatgcttcgc gctttgaatc agctcgcgcg cgaattattg ctcgcgcaat
     5281 cgagcgattg ggctttccag atttatcagg gcacgaccgt cgagtacgcc tcgcgccgct
     5341 tccgctcgca tattcaccgc tttaatcttc tggcgacgca aatcgagaaa ggcgaaatca
     5401 acgaggagct tttagcggaa atcgaatcgc gcgacaatat ttttcaggag attgattacc
     5461 ggatttacaa atcctgagat ttagatgtca aaagccggga ggctttaagg caattccatt
     5521 ttttgccggg catcattccg gtcgataaat cttccgacaa aaactttttt gacgattccg
     5581 tcctgaatca ggaattttgt cgggacgcct tttaccaaaa gagaattcgc gaattctctt
     5641 ttgtcgtcaa tcaggacggg aaaatttata tccttgtttt cggcgagcgc gttttgtttt
     5701 tctttcgggt cgacatttat accgtaaatt tgtatctgcg agcttatcgt cggatacatt
     5761 tcggctatct gctgaatttc ggctttgcag gctgtacaat cggcggaaag aaacattatt
     5821 aaaactttgc ctttcgacaa ctctttatta tcgaaaattt cgccgttcgc tgtcgaaaga
     5881 ttcgccgccg gcagatttcc gccaattaaa ttagcctcgg gttttcgtgg agtgttattc
     5941 cgccaaaaaa atatcgcccc gaaagtcagc ccgaatccga gcagaagcgc cgccagtgaa
     6001 atagatattt tttttaacat cacaaaaata tttcaaataa agaggctaaa aagtctctat
     6061 tttaagtgtc tgcacatctt aaaatagaga ctttataaaa tagaccggcg tcgagctttt
     6121 cgttaatgac aatcttcggg attcgatacc gtgcacggat tatcaaaacc gcagtaagtc
     6181 acgtaagttc cttcaggctg cggcgtattc aggtcggtcg gcaggcagga atagtcacac
     6241 gtttgtgcgt aagggtcggc gtaggtcagc gaacaaagct ccgaagttcg gtagtcggta
     6301 taataatcta tacaggttat acacggaccc atatttgccg agtaagccgc cgccgtagcc
     6361 agcaccgcac cgataaaaaa cgagaaaaaa atcattttta cagatttcat tttgcctcct
     6421 gaattaagta agaatttgat agaagtcggg tcgtggatag tttaattaaa gtgaatttgc
     6481 aagtcaagaa ttttccgcga gccgctcgaa aattgcagcc gcgccgccgg atgataattt
     6541 cggcaattgc ttctatcaat cttttcctgt ttgccttaat attttcgtaa agttatcatt
     6601 tccttttact ttcacgggag aaaaatcaat ggcaaacaaa ggcgtggaga ttgcgcccgc
     6661 aaatggtaag ctcggaattc tgctcgtcgg tctcggcgcc gtcagcacga ctttcgtggc
     6721 gggcgtcgag gcgattcgga aaggcgaggc gaaaccgatc ggcagcctga cgcagatggg
     6781 aacgattcgt ctgggcaagc gcacggagaa tcgcgcgccg cgcatcaagg attttgtgcc
     6841 gctcgccgat ttaaacgaca tcgttttcgg cgcgtgggac attttttccg attcggctta
     6901 tgaggcggcg ctgcacgccg gagttctgga aaaatcgcag attgaagatt tgcgcgagca
     6961 gctcgaatct ttaaagccga tgcaggcggt tttttcgcaa aattacgtca agcgcctgaa
     7021 cgggacgaac atcaaaggcg gcaaaaacaa aatggagctc gccgagcagc ttatcgaaga
     7081 catcgcgcgt tttaaacagg aaaaaggctg cgatagattg gtgatagtct gggcggcttc
     7141 gaccgaaatt tttcttgagc cgtcggaagt tcatgaaact ctggaagctt ttgaaaaagg
     7201 cttgtacgac gacgacgagc gcatcgcgcc ttcgatgatt tacgcttacg ccgctttgaa
     7261 atccggcgtt ccttttgcga acggcgcgcc gaatctgacc gtcgatattc cggctctgac
     7321 caggcttgcc gagcagaacg cagtgccgat ttgcggcaag gatttcaaaa ccggtcagac
     7381 gtttatgaaa accatactcg cgcccggatt gaaagcgcga atgctcgggc tggcgggctg
     7441 gtattcgacc aatattttag gaaaccgcga cggcgaagtt ctggacgacc cggaaagctt
     7501 taagaccaaa gaagaaagca aattgtccgt tttggagcat attctccagc cggaagttta
     7561 tcccgattta tacgaaggct tctcgcacgt cgtccgcatc aattattatc cgccgcgcgg
     7621 cgataacaag gaaggctggg acaacataga tattttcggc tggctcaatt acccgatgca
     7681 gataaaagtt aattttctgt gccgcgattc gattctcgcc gctccgctcg ttctggattt
     7741 ggctttgttt ctggatttgg cgcagcgcgc ggggatgaaa ggcggcgtgc aggaatggct
     7801 ctcgttttat tttaaagcgc cgcagaccgc gccgggactt tatccggaac acgatatttt
     7861 tattcaatta aaaaagctca aaaatactct gcgtcatttg cagggcgagg aattaattac
     7921 gcatctcgga ctcgaatatt acgattaaag cgaaaaagcg aaaaggcgaa aaagcggaaa
     7981 agcaggtcga gatttgcccg tccgcttttt cacgctttcg ctttttcgct ttttcacttc
     8041 gcatttttct cttgcagtat tttaattttt gtgcaaaata tgtgttaacg agtcaactga
     8101 ctcgcgcgac tcaaaaacaa gtctaagttt gaatcataaa caccttccgt gaacctccaa
     8161 aaggttcagc caacaaattt ttttttgaca gcatcttaag taagcgactt taaccgcgat
     8221 tggtttcacg gcaaagtcca agttgaaagt tcgtctatga cagtagcaaa ttgctgaatc
     8281 caaaagcgtt ttctaaggcg aggagaaata ctatatgagt aacgaaacgg caaaaaagaa
     8341 acaagcgaca gaaacacaca ttttacttga cccggacctg aaaagcccgc gagcgaaaga
     8401 attcgaagat aaattaatgg gacaaatcgt cgggcaggaa cgcgccgtgc gccgcatgag
     8461 cggacttttc cagatttatc tggcgggtat gaacaaccct tcgcgtccca tcggcacgct
     8521 gctttttctg ggtccgaccg gctcgggaaa aacgcgcgtc gtcgaagcgg cggcggaagt
     8581 ccttttcggc gacccgcttt cggtggtcaa gattgactgt gctgagtttc aacattctca
     8641 cgagattgcc aaacttatcg gttcgcctcc gggatacctc ggacaccgcg aaacttcgcc
     8701 gatgctgacg caggaaaatc tcgataaagc tcacacggat gaaacgaaac tgactttcgt
     8761 gctttttgac gaaatcgaaa aggcttcgga tgcgctgtgg cagctgctgc tgggaatttt
     8821 ggacaaagcg acattgactt tgggcgacaa ccgccgcgtc gatttttcga aagcggtcgt
     8881 cattatgacc tcgaatttag gcgcgcgcga gatgagcgag atgatttcgg gcggcatcgg
     8941 ttttgcgccg ggcaaaggtc agcgaattca ggaagaaaac gagattgacc agaaaattta
     9001 tcgcaccgca agcgaagcgg cgaaacgcaa attctcgccg gaatttatga accgcatcga
     9061 taaagtggtc gtcttccgca gcctcaaaga acatcacctg cgggaaattc tgaaaatcga
     9121 gttaaaacag gttcagaacc gtattaccga atcagccgga acgaaattcg tctttgaatg
     9181 cacggacgac gccaaggaat ttcttctgcg cgaaggcatc gatttgaaat acggcgcgcg
     9241 ccacctgaaa cgcgcgattg aacgctttct ggtttatccg ctctcaaatc tggtcgccac
     9301 cgagcaggtt tcgaccggcg acctcgtgac gattgattac gacggcgaga agctgacttt
     9361 cgccaaacaa tcgggcaaga tgattgtcaa cgaatcggac aatttcctgt tcgaggaaga
     9421 aatggaatcg aaatccgacg cggtcggtct gccgctgccg caagcgcaag ccgccgcgag
     9481 cggaaaaggc aacaaaggcg acaatcagga aagctagttt gatttaataa aaaaactaac
     9541 gggcgaggag cgagaaatcg tttctcgctt ttgtttttgt acacaaagaa aatgaaaccg
     9601 cggatttaca cagatgaacg cggattattt acaggataga caggataatt taaaaccggg
     9661 aacgttcacg tttcttttta ttccggtcat cctgtctatc ctgtttgatt ctatgtttat
     9721 ctggtgttca tccgtggtta aattttcttt cttcgtgttt cttgtgtctt cgtattgaaa
     9781 attaatcttc ccagtctttt gctcgttcaa tcgcttttgt ccatctcgcc cgcagctctt
     9841 tcgccctttc ggctggcatc tgcggctcaa aaattttatc gacttgccac gcctgagaaa
     9901 tttcgtcttt cgatttccag aatccgacgg ctaaacccgc cagataaccc gcgccgagcg
     9961 cggtcgtttc cgtgatgcgc gagcgcagaa cgggaatctg taaaatatcg gcttgaaatt
    10021 gtagcaagcc cgtatttgaa gtcgcgccgc cgtcgacgcg cagttctttc agctcgattc
    10081 cggcgtcggc gtgcatcgcg tccagcaaat ccgccgtttg atacgcgatt gattcgagcg
    10141 cggcgcgggc aatgtgcgct tttccggttc cgcgcgtcaa accgattatt gtgccgcgcg
    10201 ccgtctgatt ccagtgcggc gttccgagtc cggcaaacgc gggaacgaaa taaacgccgc
    10261 cgttgtcggc aaccgagcca gccagcgctt cgacatcggc ggatttttcg ataattccca
    10321 gagcgtctct gagccattga atgaccgcgc cgccgataaa aacgctgcct tcgagagcgt
    10381 attcggtaat attgtcaatc tgccagccga ccgtagtgag taatttgttt ttcgattcga
    10441 ccggtttcgt gccggtgttt tgcagcataa aacagcccgt gccgtatgtg tttttcgaca
    10501 ttccggcacg caggcaggct tgcccgaaaa gcgcggcttg ctggtcgccc gcgtttccgg
    10561 cgattcggat tccgcttaat ttgcccggcg ttttcacctc gccgtagact tcgcttgaag
    10621 atgtaacctg cggcaaaatc gagcgcggaa cgtcaaagat ttccagaagc tcttcgtccc
    10681 agtttaaatc gcggatgtta aaaagcatcg tgcgcgaagc gttcgagacg tccgtcaggt
    10741 gaagctcgcc gcccgtcatt ttccaaatca gccacgaatc tatcgtgccg aaacaaagct
    10801 cgcccttttc cgctaacacg cgcgcatttt cgaccgtttc caaaatccag ttcagcttgc
    10861 tggcgctgaa ataagcgtct acttcaagtc ctgttttagc gcgaatcaaa tccgcgtatt
    10921 tcgctcgaat cccgtcacaa aacgccgaag tgcggcggtc ttgccagacg attgcgttat
    10981 aaaccggctc gcccgttttt cgattccagc aaagggtcgt ctcgcgctga ttcgtaattc
    11041 cgattgcctc gatgtccgcc gcggttaaat tagctttttc gagcgcctca atcgcggttt
    11101 gcgactgcgt ttcccaaatc tcctgcggat tgtgctcgac ccagccgctg cgcggaaaaa
    11161 tctgcgtgta ttccttttgc gaaagcgaaa cgatgcggct ctctttatcg aaaacgatgg
    11221 cgcggctgct cgtcgtgccc tggtcgagag cgagaatata tttcataaaa ttcctgaagc
    11281 gaaacaacgg attagaacag attgcagagg cggaagaggc ggaaaccgaa gcgtcggcac
    11341 aggcgctgaa actcagcaaa ttcagatgct gaaagtagtt tgcgcccttg ccggtatgcg
    11401 ggcttccgcc tcttttatcc gcgccgaatc tgcgctgcgc cgtcgtttca aaattaaaaa
    11461 ggacgattct gcaatcgccc gatttcttac ttaatttgaa atttaaacta gccgataaac
    11521 atctcgtcgt ccatataaac gcggtcaatc tgtttcggcg cgggcgcgag cagaacttcc
    11581 agacctttgc gaaagccagc cataatggcg gcaatctcgc gcgccggctc gaccactttg
    11641 tgctggataa aatccgtcgt cgaagcgacc tgcgcgtgcg tccggtcaat cgttttgctg
    11701 accagctcga ctttatcttt tgccacaatc gccgtttgcc cgaccagaag cttcagctcg
    11761 gcgacttcct gttttatcag cgcggtcgat tcggaaaagt ttttcgaggc ggtcgaaaga
    11821 tgattcgaga cttctgtaaa ctggacggaa atctggcgtc cctgctcgcc gatggcgttg
    11881 accttggtca tcagcgggtc gattttatcg agtatcgggt cgattttttc ttcgacgcgg
    11941 ctgaccgtgc cgatgacccg ccggacaata aaggcaatcc aaaatagcgc aatcgccatc
    12001 acgataaagc aaatcgcaat cacgattgac gcaatcatca tccaaaactg tggttcggtt
    12061 gcgttcataa ttaatttttg gcggcggtca gatgcggtct aaccttccgt ttcatcgccc
    12121 gttttcggcg cgttcaagcc tttgctctcg accggataag tcgggcgttc gggaactgcg
    12181 gcggtatcgg tgcggcgttt ctcgtcgctg taagcctgtt ttcccgcttc gagcgcggcg
    12241 gaaaacgggt tggcggtctg cgccaccgcg cctttggctc gctcggtcaa ttcgcccgct
    12301 ttttcctgcg ccgccgagta aagctcgccg gctcggtcgc gcgtcacttc gtaatattcg
    12361 ccggcgcgtt cctgtatctg cgtcgccgct tccttgcctt tttccacgcc tttgcgcgtc
    12421 acgtcggcga tgtctccgcg caattcgtga cccgatttcg gagcgaaaag cagcgcgaca
    12481 atcgcgccga ttccgccgcc gataagcaaa taagtcaatt tcgcagtggc gctggtctct
    12541 tcctgtcttt cgtttgtcat aatcttaaat tcctctcaaa tagttgtaat cattcaaatg
    12601 ctaaaaaatt tgtaaaaatg gatacattga atataacaga aattctttat tatgagcaat
    12661 attattaata aaaggataaa ggctaatcga taaaggataa agactaagga aagatttttt
    12721 tttctgcttc gtcctttcgc ctttcgcctt tcgcctttat cctttcaaac gcgctctgac
    12781 cactcgcgga atgccctgca aatccggcag aattttcacg ctctgccacg tttccgcccg
    12841 aaacatttcc cgcactttcg ccgcctggtt aaatccgatt tccaacagca aaaatccgcc
    12901 gggctttaaa aaaaacggcg actcgcggat gattttttcg atgattgaaa gcccgtctgc
    12961 gccgtcggtc aaagccgtca gcgggtcgaa atttctgact tccggctgca aggcttcgat
    13021 gtcctgacgc ggaatatacg gcggattgga gacgattaaa tcgaattttt ccgtttcgag
    13081 agcggcgaaa acatcggaga ttttcagacc gagacgattt attactccat tatttaccgc
    13141 gtttagcttg gcgatttcaa ccgctttttc cgaaacgtcg aagccgattg ccgacgccgt
    13201 ttttatctcg tgcagaatcg agaccgaaat acagcccgaa ccgacgccga cttcgcaaaa
    13261 cgccggattt tctttatcac gcaaaatctg gcaggcgttt tcgacaatta attccgtttc
    13321 cgtacgcgga atcaaaacgt ctttcgtgac gacaaaatcc agcccgtaaa attcctgtct
    13381 gccggtgatg tgttgaaaag gctcgcgggc ggcgcggcgg cgcagaaatt cacgaaaccg
    13441 cttttcttct tcgcctgaaa gctcgtattc cggacgcgcg ataagaaagg ctttatcttt
    13501 ccgcaaagcg aaagccagaa gagagttggc ttcgcgtcgc ggctcggcga tgccgctttc
    13561 ctgtaaaatt tgcgcggcga ttttgatgtt ttcggcaata ttcgacagat taaaatcctt
    13621 tcacggcttg atcgatttca tcccgacaaa ggctggcata tatttattaa tcaaccgcgc
    13681 cgcgtcgtgc cagtaatctt cgtaaaaacc cgtcacgaca aagcccgccg cgatttgtcc
    13741 gccgatttgt tcttcgagcg tgtggctgaa ttccagcggt cgcccgcttt gcaaaatctc
    13801 gtttcgttca tcctcgccga ttgattccaa atccgaatac ggcagcgaat gacgaacgcg
    13861 caaaactcct tcctcgtctt ccgctttgct atcgaaaata aaaacttccg gcttggtaaa
    13921 tccgaccagc agcgcgccgc cgcgccgcaa aacgcggaaa cactcgcgcc agatcggttc
    13981 gagcttcggc ataaaacaat tcgagcaggg atgaaaaata aggtcgaagc cgctctcggc
    14041 aaaacgcgac aaatcagccg tgtcgccttt ctcgattcgc aaattcaaat tgtctctttc
    14101 ggcgacaaat ctgtcctgtt cgagctgttt cgccgaattg tcaaaaacgg tgacgctcgc
    14161 tcccgccgcc gctaaaaccg gcgcctgctg accgccgccc gaagccagac ataaaatatc
    14221 tttgcctttt acctcgccga accattcgcg cggaacgggc tttaccggcg tcaaaacaat
    14281 gctccaatta ccgcgccgcg cttcgtcaat cgtctccgcg ctgaccgcaa cgctccaatc
    14341 gtttcctttt tcgacttcct tgtcccaagc cgcgctgttg tattcctgaa cattcataat
    14401 tttagttgat agttgataac tgatacctga tagtgaaaaa acaaaactat cagttatcag
    14461 ttatcaggta tcagttatct tgctccgtcg ccgcgcagga tagccggaag cgtcagcaga
    14521 attatcttca aatccagcca cagcgaccag ttttcgatgt aatacaaatc gattttgacc
    14581 atttcttcaa acgtcaggcg gtttctgccc gaaacctgcc acaaacccgt aattccgggc
    14641 ttcatatcca gccgccgccg atgccttaaa tcgtattcgg cgacttcata cggaatcggc
    14701 gggcgcgcgc cgacgacgct catctcgccg cgcagaacgt ttaaaaactg cggcagctcg
    14761 tccaggctcg tgcgccgcag aaatttaccg gcgcgcgtgc ggcgcgggtc gtttttgact
    14821 ttgccgaaaa cgggcttttc ggcgtcgccc gcgtttgcct cttccgtcgc gccctcgatg
    14881 tttttgcgat acgcttcgcg gtgcagattt tcgtccgcgc ccgcctgcat cgtgcggaat
    14941 ttgtagcaga gaaaaatccg cccgtccatc ccgacgcgtt cctgccggaa caaaaccgaa
    15001 ccgcgcgaat cgagcttgat aagaatcgaa atcacaatcc agattggcgc gctcgaaatg
    15061 attgccagca aagaaattac gatgtccgaa attcttttca ccagccgctc cgcatccgac
    15121 agaggctcgc ggaaaagccg caccatcggc aaaactccga tttgctcgat gctcgttttc
    15181 tgcggcaaaa cattgaaaag attcggcgca aaacgaaact cgaccttttg tttgcgcccg
    15241 acctgcatca tcgcttcaaa aaatttctcg ctcggaattt tatcatccgt gataatgact
    15301 tcctgaattt caagctcgcg aatcaaatcc ggcaaatcgt caatcgtgcc gataatatta
    15361 gttccgggtt tcaggttcca attttcaata tctttctccg cgtctccgcc tctccgcgtc
    15421 tccgcgtttg cgcttactat tccaatcacc ctgtaaccca aatcggggcg ctctttcaat
    15481 tcccgaatcg tctgctcggc ttccgcgttc gtaccgacaa tcaaagtcgg aatcaggttg
    15541 atgccgctcg ctctgaaaat cgtctgcgca tagcgcagag caaggtgaaa cgccgtgaaa
    15601 acaacgagcg ccaccacaaa atcgagcaga aaaactccgc gtgaatacga aaagtcacga
    15661 aaagcgaaac cgccgcgaaa cagaaacgtg aaagcgatta tcaggagcga gccgaccgcg
    15721 acggctttga aaaccttgat aaattcggcg atgtaggaaa acgcgccctg aaggcgataa
    15781 actctttgat aagaaagcat cgcgagccgc accggaacgg cgaaaaagac gactccggcg
    15841 tatggcacaa actctttcga
//
LOCUS       DASWNE010000002         2063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_106077, whole genome shotgun sequence.
ACCESSION   DASWNE010000002 DASWNE010000000
VERSION     DASWNE010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106077"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(303..>2063)
                     /locus_tag="VGB68_00080"
     CDS             complement(303..>2063)
                     /locus_tag="VGB68_00080"
                     /inference="COORDINATES: protein motif:HMM:NF014180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047652.1"
                     /translation="VPAAEGGEKESCDVWSGTIEITRRRAESHIKVTNKGEHISDQHY
                     SGKAEIKSSYDYKGIFNVSNSTGGSTYEDGSKTLSLKGTVSAEALRVQEEKDNWKTET
                     DCFPDTPRLRVAGKDSFSSIAESGQLPKTRGDGVMSIKGNRYTIGFGIPQIEGKRVHK
                     TTVKPYGWCMMEMNPPHDSTDESAETFASERVEIEGSLDPQNPDVLQGKSQPDEETTI
                     VWNLTRKPGECDEPLQINDLRLAHHVFPNKTDWEDIGENTVDGNEVRITATVVNEAKK
                     AKTGTVVFKETKSGEVLGEKPVSVPAESETEVEFLWDTNGYAWTEAGKNGTNREIEAS
                     IAGADSETSEVKVYPKPVILVHGLWSNGAAWGEYHNYLEEAHSFAWKAYAVGADPKVA
                     MMSTGKSAGNMEPTFSIRQNAQELGKQITHTQKTENAWRVDVVAHSMGGLISRYYIHH
                     SMTNAPDGKPTIAHLVMLGTPNMGSPCADFLFPKYKLLGFEVEALRELQRPVVAEFNK
                     QITNRKGVRFSITYTDKVPLTCDLKEAGDGVVTRASAIWEIADVSHSDSLEHTSLTGK
                     EDFMRFVRPRLALGPKMAKY"
BASE COUNT          390 a          571 c          525 g          577 t
ORIGIN      
        1 tagaccccgg cgcttttgtt acaacacact cgctgtcgga agtagaaggc ggtctggtta
       61 aaaaatcagg cttacattaa ttcatcttaa atattttatt cagacccgaa aaccgtcttt
      121 tatactatct cggaaacggg cgtttttgag acggcttaaa gttatcgatt gcctctcgcc
      181 tgcaattccg gtatcgggcg gctccattat taatttaagt aaatcaacag cttgcataaa
      241 aaataaaggc atcaaaaatg atgccttttt ccttttgtgc ggcaaactga aaaaataact
      301 ttttaatact ttgccatttt cggtccgagc gcgaggcgcg gacgcacgaa gcgcataaaa
      361 tcttctttgc cggtcaggga tgtgtgttcg aggctgtcgg aatggctgac gtcggcaatc
      421 tcccagatcg ccgatgctct cgtcaccacg ccgtcgcccg cttcctttaa atcgcaggtc
      481 agcggaacct tgtcggtata ggtgatggag aatctcacgc ctttgcgatt ggtaatctgt
      541 ttgttaaatt cggcgacgac cgggcgctga agctcgcgca gggcttcgac ctcaaaaccg
      601 aggagcttgt atttcggaaa caggaaatca gcgcacgggc tgcccatatt cggcgtgccg
      661 agcatcacca gatgcgcgat ggtcggcttg ccgtccggcg cgttcgtcat cgaatggtga
      721 atgtagtagc gggaaatcaa gccgcccatc gagtgcgcca ccacgtcgac gcgccacgcg
      781 ttttcggttt tttgcgtatg cgtgatttgt ttgccgagtt cctgcgcgtt ctggcgaatg
      841 ctgaaggtcg gctccatgtt tccggcgctc ttgcccgtgc tcatcatcgc tactttcggg
      901 tctgcgccga cggcgtaagc cttccacgcg aagctgtgcg cttcttccag ataattgtga
      961 tactcgcccc aagccgcgcc gttgctccat agtccgtgaa ctaaaatgac gggtttcgga
     1021 taaactttga cttccgatgt ttcgctgtcc gcgccggcga tgctcgcttc gatttcgcgg
     1081 ttcgtgccgt ttttaccggc ttccgtccag gcgtaaccgt tcgtgtccca caaaaattcc
     1141 acttccgttt cgctttcagc cggaacgctg accggctttt cgcctaaaac ctcaccgctt
     1201 tttgtttcct tgaaaacgac cgtgccggtt ttcgctttct tcgcctcgtt gacgacggtc
     1261 gccgtgatgc gaacctcgtt tccgtcaacc gtattttcgc cgatatcctc ccaatcggtt
     1321 ttattcggga aaacgtgatg cgccagtctt aaatcgttaa tctgcaaagg ctcgtcgcat
     1381 tcgcccggct tgcgggtcag attccagacg atggtcgttt cttcgtcggg ctgcgatttg
     1441 ccctgcagga cgtccgggtt ttgcgggtcg aggctgcctt cgatttcgac tctttcgctc
     1501 gcaaatgttt ccgcgctttc atcggtcgaa tcgtgcggcg gattcatctc catcatgcac
     1561 cagccgtaag gtttgacggt ggttttatga acgcgcttgc cctcgatttg aggaattccg
     1621 aaaccgatcg tgtagcgatt gcctttgatg ctcatcacgc cgtcgccgcg cgtcttcgga
     1681 agctgcccgc tctcggcgat gcttgaaaac gaatcttttc cggcgacgcg caggcgcggc
     1741 gtgtccggaa agcaatcggt ttcggtcttc cagttgtctt tttcctcctg cacgcgcagg
     1801 gcttcggcgc tgaccgttcc tttgagcgac aaggtttttg aaccgtcttc ataagtgctg
     1861 ccgccggtcg aattggagac gttgaaaatc cctttatagt cgtatgacga ttttatctcc
     1921 gcctttcccg aatagtgctg gtcgctgata tgctcgcctt tattggttac cttaatatga
     1981 ctttcggctc ggcgacgagt gatttcaatc gtcccgctcc aaacgtcgca gctttccttt
     2041 tcgccgcctt cggcagccgg aac
//
LOCUS       DASWNE010000003         3648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_70786, whole genome shotgun sequence.
ACCESSION   DASWNE010000003 DASWNE010000000
VERSION     DASWNE010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70786"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..761)
                     /locus_tag="VGB68_00085"
     CDS             complement(78..761)
                     /locus_tag="VGB68_00085"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEY0047653.1"
                     /translation="MKLAIFDIDGTLTETNEVDNECFVRAFAESHQISDIETDWTKYK
                     HVTDSGILLEIFETKLERTPKEKDFAAFKHCFIKNLKEFAGKDAKLFAEIPNAKIMLE
                     KLRLEKDWAIALATGCYYDSAELKLRAAKLNIKDFPIATADDAVTREEILRIAIGKSL
                     ERYRQNEFEKIFSIGDGVWDLRTAKNLNLEFIGIAGGERAEKLRREGAQFLIEDFRDY
                     KVFLEYLNK"
     gene            complement(758..2374)
                     /locus_tag="VGB68_00090"
     CDS             complement(758..2374)
                     /locus_tag="VGB68_00090"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HEY0047654.1"
                     /translation="MTATGLLIIVTLFAVPLFFSLPRVGGAGFGKSLSGATTGFSDSI
                     RLGEIARVQQNNEVVMRVRIDEADKHKVQSIRWRGVALDFFDNYGWRKTRLNFNEPFV
                     KTERDFFQVGTASNANNLVTQTVYLEPLDTPILFSLSRPVAFQGSFQLLTKDAEGSIS
                     VSRSGGERFSYKAYSDVTQPSAEKLKNDNAVYAQQQKRYLQLPAQPIDARIGNLAAEI
                     IRSANADNRYDKARAIEFYLQNNFGYTLDLRASGEEPLADFLFNVKEGHCEYFATAMA
                     VMLRTQGIATRVVNGFQTGEYNETADIYVVRQREAHSWVEVYFPQENAWVTFDPTPAA
                     GRFSDENATGIIGSFNKYLEALETFWIQYIVSYDNQEQKSLLRSVRSSVQDYQIEATT
                     WANLLQLRLADWWKQVRGDEGFAKSAWAIGYGVLYLLGFALGIFAIVRLYRKSKKLKM
                     WEKLKAFFRREKEDSIVEFYERMQNVLARKGFERATHQTPLEFAFALNMPEAVKITEK
                     YNRVRFGEKNLSRDEAQQIESWLKNLEGKQ"
     gene            complement(2401..2895)
                     /locus_tag="VGB68_00095"
     CDS             complement(2401..2895)
                     /locus_tag="VGB68_00095"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HEY0047655.1"
                     /translation="MNFDKFFKLISYAVGLCGLFALTVSGGVGILVSAVFIFVAILAW
                     FIEDTRWQLSERIGIVLVFLFIPLFYLDWRYQLSGFAARETLAAGSLARLILVLAGIK
                     LLQRKSDRDWIFLYLISFFEILLAAGLSISPLFILALAAYMLFVVCAIVTFEIKKAKS
                     IIST"
     gene            complement(3017..3133)
                     /gene="rrf"
                     /locus_tag="VGB68_00100"
     rRNA            complement(3017..3133)
                     /gene="rrf"
                     /locus_tag="VGB68_00100"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(3248..>3648)
                     /locus_tag="VGB68_00105"
     rRNA            complement(3248..>3648)
                     /locus_tag="VGB68_00105"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          956 a          929 c          777 g          986 t
ORIGIN      
        1 ttcttttaat tgtcccatca tcacttccga atcgacgaga ttcttcggac aaccgaggct
       61 gacaaatcct acttttttca tttgtttaaa tattctaaaa aaactttata atctctaaaa
      121 tcttcgatga ggaattgcgc gccttcccgg cgcaattttt cggcgcgttc gccgccggcg
      181 atgccgataa attctaaatt cagattcttc gccgttcgca ggtcccaaac tccgtcgccg
      241 attgaaaata ttttttcaaa ctcgttttgc cggtaacgct ctaaggattt cccgattgca
      301 atccgtagaa tttcttctct cgtaaccgcg tcgtccgccg tcgcaatcgg aaaatctttg
      361 atatttaact ttgccgctct taatttcagc tcggctgaat catagtaaca tccggtcgcc
      421 aaagcaatcg cccagtcttt ttcgagcctg agtttttcga gcattatttt cgcgtttgga
      481 atttcagcga agagttttgc atctttaccg gcaaattcct tcaaattttt gatgaaacaa
      541 tgtttgaagg ctgcgaaatc tttctctttt ggcgttcgtt ccagctttgt ctcgaaaatc
      601 tccagaagaa ttccggaatc ggtgacgtgt ttatattttg tccaatccgt ttcaatatcg
      661 cttatttgat gcgactcggc aaatgctctg acgaaacatt cattatcaac ttcgttggtt
      721 tcggtcaggg tgccgtcaat atcaaatatc gccagcttca ttgctttcct tctaaattct
      781 ttagccagct ttcaatctgc tgcgcctcat ctctcgataa attcttttcg ccgaagcgca
      841 cgcgattata tttttccgta atcttgacgg cttccggcat attcagcgcg aaagcgaatt
      901 cgagcggcgt ttgatgcgtg gcacgctcga agcctttgcg cgccagaacg ttttgcattc
      961 gctcgtaaaa ctcgacgatt gaatcttctt tttcgcgcct gaaaaatgct ttcagctttt
     1021 cccacatttt cagttttttg gattttcgat aaagccggac gattgcgaaa attcccagcg
     1081 caaaaccgag cagatacaaa acgccgtaac cgattgccca ggcgcttttc gcaaagcctt
     1141 cgtcgccgcg cacctgtttc caccaatcgg cgaggcgcaa ctgcaaaaga ttcgcccagg
     1201 tcgtcgcttc gatttgataa tcctgaacgc tgcttcgaac cgagcgaagc agcgattttt
     1261 gctcctgatt atcgtaggaa acgatgtatt gaatccagaa agtttcgagc gcttcgagat
     1321 atttattaaa gcttccgata atgcccgtcg cattctcatc cgaaaatctt cccgccgcgg
     1381 gcgtcgggtc aaacgtcacc caggcgtttt cctgcggaaa ataaacttca acccacgaat
     1441 gcgcctcgcg ctggcgaaca acgtaaatgt cggcggtttc attatattcg ccggtttgaa
     1501 aaccgttgac gacgcgcgtc gcgatgccct gcgtcctgag cattaccgcc attgccgtcg
     1561 caaaatattc gcaatgccct tctttaacat taaacaaaaa atctgccaga ggctcttcgc
     1621 cgctcgctct taaatccagc gtgtagccga aattgttttg cagatagaat tcgatggctc
     1681 tggctttgtc gtagcgatta tcggcgtttg cgctccggat tatttcggcg gctaaattgc
     1741 cgattctggc gtcaatcggc tgtgccggca actgtaaata acgtttctgc tgctgcgcgt
     1801 aaactgcgtt atcatttttt aatttttcgg cgctcggctg cgtgacgtcc gaataagctt
     1861 tataagaaaa tctttcgccg ccgcttcggc taacggaaat cgagccttcg gcgtctttag
     1921 tcaataactg gaaactcccc tgaaacgcga ccggacgtga gagcgaaaac aaaatcggcg
     1981 tgtcgagcgg ctccagatag acggtctgcg tcaccagatt attagcgtta gatgcggttc
     2041 cgacctgaaa gaaatctctt tcggttttaa cgaatggctc gttgaagttg agtctggtct
     2101 tgcgccagcc gtaattgtcg aaaaaatcca gtgccacgcc gcgccacctg atactttgga
     2161 ctttatgctt atcggcttcg tcgatgcgga cacgcattac gacttcgtta ttctgctgaa
     2221 cgcgcgcgat ttcgccgagc cgaatcgaat cggaaaaacc ggtggtcgcc ccgctcaggc
     2281 ttttgccgaa acccgcgccg ccgacgcgcg gcagggagaa aaataaagga acggcaaaga
     2341 gcgtgacaat gatgagcaat ccggtcgccg tcacaggcag gcgaaaaatg gttttcctgt
     2401 ctaagttgag ataattgatt ttgctttttt gatttcaaac gtgacaatcg cgcaaaccac
     2461 aaaaagcata tatgcagcca gcgccagaat aaacaacggg ctgatgctta agccagcggc
     2521 gagaagaatt tcaaaaaatg aaatgagata aagaaaaatc cagtctcggt cgctctttct
     2581 ctgcaggagc ttgattccgg caagcaccaa aatcaaacga gccagactgc ccgccgcaag
     2641 cgtttctctg gcagcaaaac cgctgagctg ataacgccaa tcaagataaa aaagaggtat
     2701 gaaaagaaaa acaagaacga ttccgattct ttccgacaac tgccagcgag tgtcctcgat
     2761 aaaccaggct aaaatcgcga caaatataaa aaccgccgaa accaaaatgc cgaccccgcc
     2821 cgaaaccgtc agggcaaaaa gcccgcacag cccgaccgca taagaaatta atttaaaaaa
     2881 tttgtcgaaa ttcattgagt tttcggaagt tataccgatt ttgattataa ctttcggatg
     2941 aaatgttgtc caagtttttg ccggaaacag aaaaagcccg ttcaataaag aacaggcttt
     3001 ttcgtttaaa atataaatcc ggcggcttcc tactttccca cactggaaac agtgcagtat
     3061 catcggctct aataggctta acttccgtgt tcgggatggg aacgggtgtg accctaccga
     3121 caaaaccacc ggaaaactta aaatatcaaa aaaaatttga atatgacaat tgaatgattt
     3181 ctcttagtac tattacttct tcttagttgc gtaactaaac cgtaaatttt gctattcaac
     3241 aaattttatg gtcaagcctt gggacaatta gtattggtca actaaataca ttactgcact
     3301 tacatctcca acctatcaac gtcgtggtct acgacgagtc tcactggcag aactaatctt
     3361 gagtctggct tcacgcttag atgcattcag cgtttatcca tgcttgactt agctaccgtg
     3421 cgctaccgct ggcgcgataa cacgtacact agaggtcaat ccatcccggt cctctcgtac
     3481 taaggacaga ttctctcaat tctgctacgc ccacaccaga tagggaccga actgtctcgc
     3541 gacgttctga acccagctca cgtacctctt taattggcga acagccaaac ccttgggacc
     3601 ttcttcagcc ccaggatgag atgagccgac atcgaggtgc caaaccct
//
LOCUS       DASWNE010000004         9820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_565336, whole genome shotgun sequence.
ACCESSION   DASWNE010000004 DASWNE010000000
VERSION     DASWNE010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9820
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565336"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..1108
                     /locus_tag="VGB68_00110"
     CDS             155..1108
                     /locus_tag="VGB68_00110"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEY0047656.1"
                     /translation="MSANDIFLNQLGRLRSGWRFLIFQLLIMFLGAATGAAVGLIFQN
                     LSPDYREAGALSFISQNVILLFPAVLCGWLCGKFLEDLPFRALGAAFTKNWLRDFLAG
                     WIIGAISISIAVVIALVFGGMKLEFNQSAGRSAILLTLSVSLLVFTIAAAAEEAYFRG
                     YILQTFARARLAWLAIAFTSLFFAFAHFNNPSANRVSIINTALAGIWFGVAYLKTRNL
                     WFPFGIHLAWNWLQGAFFGIPVSGITELTTAPLFQQSEKGSDFLTGGQYGLEGGIGCT
                     IALILSTVLIYFLPLLKPTEEMMALTSEEKMRESKAIIQTG"
     gene            complement(1180..2037)
                     /gene="nadC"
                     /locus_tag="VGB68_00115"
     CDS             complement(1180..2037)
                     /gene="nadC"
                     /locus_tag="VGB68_00115"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEY0047657.1"
                     /translation="MSWLDTGAIMGQIGEFLAEDLGRGDITTQATVPDDVRGVGRFLA
                     KEDLVVCGIEIAEAVFMHLDAESPELEPVFNDGDEVKAGTVFAAQKGFADVLLAGERT
                     ALNLIQRLSGIATLTRQYVRAVEGTDAAIVDTRKTTPGLRMLEKYAVTVGGAKNHRLG
                     LDDGVLIKDNHITMAGGVKEAVRMAKQKIGHLHKVEVEITNWAQLREAVLAGADIIMI
                     DNQTPDEAAKLVEMSRNMNPNVLIEASGGMNLDTVRSYAEAGVDLISVGKLTHSARAV
                     DISFKIQTL"
     gene            complement(2620..3348)
                     /locus_tag="VGB68_00120"
     CDS             complement(2620..3348)
                     /locus_tag="VGB68_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047658.1"
                     /translation="MSGNYGFIKKLNAAAIVVLTLCAALGVAAQESAFKEEDLKVLIE
                     NAAEKLDGKTYRLKTASVNYQGENSTPVYTRKTISEFIPPERERIVTETQTAEGIKRY
                     ESIRIGEKKFVRYNNGEWQEADGTEMGTSYGTGDGSLSAKVETTTERRFRKGEIVNNQ
                     TADFYETVTTTKFIYPAKTYTSVSRIAYWFDVNGQFVKSENEYQSDMDTKSIFRMTRE
                     YEYDANIKIEAPLIRSKPKVKDKP"
     gene            complement(3371..6187)
                     /locus_tag="VGB68_00125"
     CDS             complement(3371..6187)
                     /locus_tag="VGB68_00125"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEY0047659.1"
                     /translation="MEIAKAYNPKEAEESHYQNWESKGYFAPEINENPDAEPYSIVIP
                     PPNVTGSLHMGHALQHTLMDTLTRWKRMRGYRALFVVGVDHAGISTQLMVTRQLKQEE
                     KKTPQDIGREAFTERVWAWKAKYGGEITRQIRREGLSVDWSRERFTMDESLSRAVREV
                     FVRLYDEGWIYRGNRIINWCPRDKTVLSDLEVKEETKKDGKLFYLQYPIKNSDKKITV
                     ATTRPETMLGDTAVAVNSEDGRYGDLIGQTIELPLANREILIIADDYVDPEFGTGAVK
                     ITPAHDPNDYEVGLRHNLEQLNVINKDATMNAAAGAEFEGLDRYAAREKVVAKFEELG
                     LLEKVVDYEMTLPHCERCKTVIEPLLSEHWFVKMDEMRDMALDLMRREKAPRFFPEVP
                     YEKIYTTWLENLRDWTISRQLWWGHQIPAWYDAQGSVYVARTKEKASEQAGTSDLTQD
                     PDVLDTWFSSALWSFTTLGWNGEVTETEDLKTFHPTDVLVTNRDIIFLWVSRMVMTAL
                     KFLGEKPFKDVIIHGTVLDKNGQRMSKTKMNGIDPLDVFEKFGVDATRMALAGSATGA
                     DFAWRDEKVESFRNFANKIWNATRFCLLNSDGAQVDYTFLNPRAEALEIQNLISDELS
                     ELDEEDEEFLDKVVPEFAADNPKENLSIADRWIISRLNKTALSVNRALETYQFHEAVQ
                     LLYHFFWDDFCDWYIELVKDEITAPEPDVGRDAARSRIITILEQALRLLHPFMPFLTE
                     ELWQKLPEVSPALHNPAYKNAEQTIMLADFPKGDAALIDEKAEGEMQAVIELISKTRN
                     IRAEMNIKPSDKILLLVSAGGKLQKIFAENEAQILKLARASELRISENAQIPKASARA
                     VLTGGAELALPLEGLIDFQKETERLENQLNKLETENERLQKQLSNQNFVEKAPAEKVQ
                     EIRDRAAEIETQMKNLNENLKALQ"
     gene            complement(6351..7652)
                     /locus_tag="VGB68_00130"
     CDS             complement(6351..7652)
                     /locus_tag="VGB68_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0047660.1"
                     /translation="MARRKILLADDSVTIQKVVNLTFADEGIDVFPISNGNEAIDRLL
                     EISPDLVMADVHMPGYSGYEVCERIKQHEKFKNTPVILLIGSFETFNEQEARRVGADD
                     YLTKPFQSIRQLVTKVSELLERSETENVEATPTAPENSFAETREMEPPIQAEVIESET
                     PRYEDAGFDDEMIQTNQPSSFAFDESQKFASRNYVEPREEDYGKTQPLSAADLREIEA
                     APDAYDSPAQEESTPQSEQAASSEISQSSQPASRVESDDDDDFLELFDDDEYEDEDEE
                     ETESVQPVAETASPNEAGAESRTAVSEAEDYQTAETENVQTTEMQTVEPVEAETAEAS
                     ETQSIQPEEAENYPVESESVQPVETQTAQTSETGNAQPNEREAAAASASSQLSPEMID
                     AIAQRVIEKLSASAVKEIAWEVVPQQADLIIRKMVEEKLKE"
     gene            complement(7764..8171)
                     /locus_tag="VGB68_00135"
     CDS             complement(7764..8171)
                     /locus_tag="VGB68_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0047661.1"
                     /translation="MNEETQDGLPQVLVADDDPAILRLVKAIVEKEGYTVITARDGRD
                     AYKLLQSGEPFVAAIFDVVMPYIQGTELVRYMQSEKRLMKIPVIVMTAEQNPRLSSDS
                     FAAGAVAFLPKPFTTVQLQTMLKMFVQKSRNSA"
     gene            complement(8296..9363)
                     /gene="pdxA"
                     /locus_tag="VGB68_00140"
     CDS             complement(8296..9363)
                     /gene="pdxA"
                     /locus_tag="VGB68_00140"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEY0047662.1"
                     /translation="MKTSSKENLSETSSTNKNPQRVAVTIGDAAGIGAEITLKAISDA
                     RIRAACQPVIIGDAKFLLETARKFGLFDDFAVVRKGARIPVNSGKPVIYDLANLNEKI
                     TPGEESAATGRASAEYIETAVALWKENRIDAIATAPISKKALALGGYDFPGHTEFLAA
                     LTNTKDFAMSFFAEKLRVVLLSTHVSLVEAINLVKKENLVRLILFTNRELEKLLKRDV
                     KIAVAGLNPHASEGGMFGAEEADEIAPAISECREKHGVDVSGAYSPDTIFLRGYKGEF
                     DAVVACYHDQATIAVKCLSFGASVNVTLGLPLIRTSVDHGTAIDIAGKNIAEASSMKA
                     AINLAAHLSEEQTKNRAAESM"
     gene            complement(9391..9660)
                     /locus_tag="VGB68_00145"
     CDS             complement(9391..9660)
                     /locus_tag="VGB68_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047663.1"
                     /translation="MPSFSRPTAPADDRKKMPPPAETNAEVYYYKKQIDSHTTMVVVL
                     QDGEEVEGTIEWYDRDALKINRKDQPNILLLKHNIKYMFKAEDRK"
BASE COUNT         2089 a         2628 c         2332 g         2771 t
ORIGIN      
        1 tgttcgacct ttgatttttg acctttgccg aattgagtca aatcaaagct caaaggtcat
       61 ttcttccaat tcgtaattcg taatttgtaa ttcgtaattg aaacacggcg ctctttgtca
      121 ggtaaaattt aatcggctaa actcaattaa atttatgagc gcaaacgaca ttttcctgaa
      181 tcaactcgga cggctgcgaa gcggctggcg atttctgatt tttcagcttc tcattatgtt
      241 tctcggagcc gcgaccggcg cggcagtcgg tttaattttt caaaacctgt cgcccgacta
      301 tcgtgaggcg ggcgcgcttt cttttatcag tcaaaacgtt atcttgttgt ttccggcggt
      361 tttatgcggc tggctgtgcg gaaaatttct cgaagatttg ccgtttcgcg cgctgggcgc
      421 ggcttttacg aaaaactggt tgagggattt tctcgccggt tggattatcg gcgcgatttc
      481 gatttcaatc gccgtcgtga ttgcgctcgt tttcggcgga atgaagctgg aattcaatca
      541 aagcgcggga cgctcggcaa ttttactgac gttaagtgtt tcgctgctgg ttttcaccat
      601 cgcggcggcg gctgaagaag cgtattttcg cggttatatt ctgcaaactt ttgcgcgcgc
      661 tcgtctggcg tggctggcta tcgcttttac ttcgctgttt ttcgccttcg cgcattttaa
      721 taatccgagc gcgaaccgtg tttccattat caacacggcg ctggctggca tctggttcgg
      781 cgtcgcttac ctgaaaacca gaaatctgtg gtttcccttc ggcattcatc tcgcctggaa
      841 ctggcttcag ggagcttttt tcgggattcc cgtcagcgga atcacagagc tgacgaccgc
      901 gcctttgttt cagcaatccg aaaaaggctc agactttctg accggcgggc aatacggtct
      961 ggaaggcgga atcggctgca cgattgcgtt gattctttcg accgtcctga tttatttttt
     1021 accgctgctc aagccgaccg aagaaatgat ggcgctgaca agcgaagaaa aaatgagaga
     1081 aagcaaagcg attattcaaa caggataaac aggcaaagaa aacaaattgc ggttcgattt
     1141 tctttatccc gtccatcctg tctgaataat cgcttgccgt tacaaagttt gaattttaaa
     1201 gctaatatcg accgcccgcg ccgagtgcgt caattttccg acgcttatca agtcgacgcc
     1261 cgcttcggca tacgagcgaa ccgtgtccag attcattccg cccgacgctt caatcaaaac
     1321 attcgggttc atattgcgcg acatttcgac cagtttcgcg gcttcgtccg gcgtctggtt
     1381 gtcaatcata ataatgtccg cgcccgccag aaccgcttct ctgagctgcg cccaattcgt
     1441 aatttcgacc tcgaccttat gcagatgacc gattttttgt ttcgccatac ggacggcttc
     1501 cttgacgccg ccagccatcg tgatgtgatt gtctttaatc aaaacgccgt cgtccaagcc
     1561 gagccggtga tttttggcgc cgccgacagt gacggcgtat ttttccagca tacgcaagcc
     1621 gggcgtggtt ttgcgcgtat cgacaatcgc ggcgtcggtg ccttcgaccg cgcggacgta
     1681 ttgcctggtt aaagtggcga tgccggaaag cctctgaatc aggttcagag cggtgcgctc
     1741 gcccgcgagc aaaacatcgg caaagccttt ctgcgcggcg aaaaccgttc ccgccttgac
     1801 ctcgtcgccg tcgttaaaaa ccggctcgag ctcgggcgat tcggcgtcca gatgcataaa
     1861 aaccgcctcg gcaatctcga tgccgcaaac caccaagtct tcctttgcca gaaatctgcc
     1921 gacgccgcgc acgtcgtccg gcacggtcgc ctgcgtcgta atatcgccgc gtcccaaatc
     1981 ttccgccaaa aattcgccga tttgccccat aatggcaccc gtatcaagcc agctcatatt
     2041 gaactaaagt atcataaata aaaggatttt tcacaaaaat gtttcgccgc cggaaattcc
     2101 tcgaacactt tctgcaaatc gggaatagaa tcgattgata aaccgataaa ttgatttctt
     2161 cgcccgcttg gagcctttat caaagcctcg acgcgtgcga aaaaataaaa aagcgtccga
     2221 gtaattaaac ccgaacgcct tccgaagaga atttatgagc tatcgaaatg cgcgccggat
     2281 ttatttccct ggcgacaccg gagcggacgc ggaatgggtt cttataattt tatcgaaaat
     2341 cgctaaggta aagacgccga tttgcctgat tttctgcggt ttacattcaa tgtaaattct
     2401 gccgctcgaa acccgaaaaa aaagtttctt ggaaattgtg atttttttgc attttcctat
     2461 tgactatctt tccgaaatcg gtctaaaatg acagcaccat ctaacccaag gctgttatcc
     2521 gacgatttgg gaaagaaagg tgtccgggac ttaatgtggg ctaagtcccg gatttttctt
     2581 tttccgccgg tttcttcaaa tccttaccgt tcccgatttt tacggcttat ctttaacttt
     2641 cggtttgctc ctgattaacg gcgcttcgat tttaatattc gcgtcgtatt catattctct
     2701 ggtcatacgg aaaatgcttt tcgtatccat atcgctctga tattcgtttt cgctttttac
     2761 aaactgcccg ttgacatcaa accagtaagc tattctcgac acggacgtgt atgtttttgc
     2821 cgggtagata aatttggtgg tggtcacggt ttcgtaaaaa tcggcggttt gattgttaac
     2881 gatttcacct tttctgaaac gtctctcggt cgtcgtttcc actttcgccg acagcgagcc
     2941 gtctccggtt ccgtagctcg ttcccatttc cgtgccgtca gcttcctgcc attcgccgtt
     3001 attgtatctg acgaattttt tctcgccgat gcggatagat tcgtaacgct ttatgccttc
     3061 cgccgtttgc gtttcggtga cgatacgctc gcgctcgggc ggaataaatt cgctgattgt
     3121 ttttctggtg tagacaggcg tcgagttttc gccctgataa ttgaccgacg cagtctttaa
     3181 acgatacgtt ttgccatcca gcttttccgc cgcattttct atcagaactt ttaaatcttc
     3241 ctctttaaac gcgctttcct gcgccgcgac gccgagcgct gcgcaaagcg tcagaacgac
     3301 gattgccgcc gcgttcagtt tttttataaa cccgtaattt ccagacatat tcaaacctcc
     3361 taaaacgcct ttattgcaag gcttttaaat tttcgttcaa atttttcatc tgcgtttcga
     3421 tttccgccgc tcggtcgcgg atttcctgaa ccttttccgc cggcgctttc tcgacaaaat
     3481 tctgattgga aagctgtttt tgtaatcttt cgttttccgt ttcgagcttg tttaattgat
     3541 tttccagccg ctcggtttct ttctgaaaat caatcaagcc ttcgagcgga agagcgagct
     3601 ccgcgccgcc cgtcagaacg gcgcgcgccg aagctttcgg aatctgcgcg ttttcgctga
     3661 ttcgtaattc gctcgcccgc gccagcttta aaatctgcgc ttcgttttcg gcaaagattt
     3721 tctgcagctt gccgcctgcc gaaacgagca gcaagatttt gtcggacggc ttgatgttca
     3781 tttcggcacg gatatttcgc gtttttgaaa tcaactcgat gacggcttgc atctcgcctt
     3841 ccgctttttc gtcaatcagc gccgcgtcgc ctttcggaaa atcggcgagc ataatagtct
     3901 gctccgcatt tttataagcc ggattgtgca aagcgggcga gacttccggc agcttttgcc
     3961 agagttcctc ggtcagaaac ggcataaacg ggtgcagcag gcgcaatgcc tgctccagaa
     4021 tcgtgataat gcgcgagcgc gccgcgtcgc gcccgacgtc aggctccggc gcggtaatct
     4081 cgtccttgac cagctcgatg taccaatcgc agaaatcgtc ccagaaaaaa tggtaaagaa
     4141 gctgcacggc ttcgtgaaac tggtaagttt ccagcgcgcg gttgacgctc agagccgttt
     4201 tattcagccg cgagataatc cagcggtcgg caatcgaaag attttccttc ggattgtccg
     4261 ccgcaaattc cggcacgact ttatccagaa attcttcgtc ttcctcgtcc agctcgctca
     4321 gctcgtcgct tatcaggttc tgaatttcca gggcttcggc tcgcggattc agaaaagtgt
     4381 aatcaacctg cgcgccgtcg ctatttaaga ggcaaaagcg cgtcgcattc cagattttgt
     4441 tggcgaaatt gcggaaagat tcaacctttt cgtcgcgcca cgcgaaatcc gcgccggtcg
     4501 ccgaacccgc caaagccatt ctggtcgcat cgacgccgaa tttttcgaaa acgtcgagcg
     4561 ggtcgatgcc gttcattttc gttttcgaca ttcgctgacc gtttttgtcc aaaaccgtcc
     4621 cgtgaatgat tacgtctttg aacggctttt cgccgagaaa tttcagagcc gtcatcacca
     4681 tccgcgaaac ccatagaaaa ataatatcgc ggtttgtaac cagcacgtcg gtcgggtgaa
     4741 acgtctttaa atcttccgtc tccgtcactt cgccgttcca gccgagcgtc gtgaacgacc
     4801 acagagcgga cgaaaaccag gtgtcgagaa cgtccgggtc ttgcgttaaa tcgctcgttc
     4861 ctgcctgctc ggaggctttt tccttcgtcc gcgccacgta aacgcttccc tgcgcgtcgt
     4921 accaggcggg aatctggtgt ccccaccaga gctggcgcga aatcgtccaa tccctcagat
     4981 tttcgagcca cgtcgtgtaa attttttcat acggaacttc gggaaaaaag cgcggcgctt
     5041 tctcgcggcg catcaaatcc aaagccatat cgcgcatctc gtccattttc acgaaccagt
     5101 gctcggaaag aagcggctcg ataaccgttt tgcagcgctc gcagtgcggc agagtcattt
     5161 cgtaatcgac gactttttcg agtaatccta attcttcaaa tttcgcgacc actttttcgc
     5221 gcgccgcgta gcggtctaag ccttcgaact cggcgcccgc cgcggcattc atcgtcgcgt
     5281 ccttgtttat cacgttgagc tgttcgagat tgtgacgcaa gccgacttca taatcgttcg
     5341 ggtcgtgggc gggcgtgatt ttcactgcgc cggttccgaa ttccgggtcg acgtaatcgt
     5401 cggcgataat gagaatttcg cgattcgcga gcggcagctc gattgtttgt ccgattaaat
     5461 cgccgtaacg cccgtcttcg gaattaacgg cgaccgccgt atcgccgagc atcgtttcgg
     5521 gtcgcgtcgt cgccaccgtg atttttttat ccgaattttt tataggatat tgcagataaa
     5581 aaagcttgcc gtcttttttc gtctcctctt tgacttccaa atccgataaa acggtcttgt
     5641 cgcgcgggca ccagttgata atgcggttgc cgcgatatat ccagccttcg tcataaagac
     5701 gcacgaaaac ctcgcgcacc gctcgcgaga ggctttcgtc catcgtaaag cgctcgcgcg
     5761 accaatcgac cgaaagacct tcgcggcgaa tctggcgcgt aatctcgccg ccgtatttcg
     5821 ctttccaagc ccagactctt tcggtaaaag cttcgcgccc gatgtcctgc ggagttttct
     5881 tttcttcctg ctttagctgg cgcgtcacca tcagctgcgt cgagatgccc gcgtggtcga
     5941 cgccgacgac aaaaagcgct ctatagccgc gcattcgctt ccagcgcgtc agagtgtcca
     6001 tcagcgtatg ctgcagcgcg tgtcccatat gcaggctgcc cgtcacgttc ggcggcggaa
     6061 tgacgattga atacggctcg gcatccggat tttcgttgat ttccggcgcg aaatagcctt
     6121 tgctttccca gttttgataa tgactctctt cggcttcctt cggattatat gcttttgcga
     6181 tttccataat ttgtcagtgg tcggcggtca gtaagtcagc ggtcagcttt tggctgattg
     6241 ccgacggctg aaagctgacg gctaaaaatt taaattctaa aacaaaaacg gctagctttc
     6301 atctcgttgg cgaaaactaa ccgctactga ttactaacta taaatatttt ctattctttt
     6361 aatttttctt cgaccatttt cctgattatt aaatccgcct gctgcggaac gacttcccag
     6421 gcgatttctt tgaccgcgct ggcggaaagc ttttcgatga cgcgctgcgc gatggcgtca
     6481 atcatttccg gcgaaagctg gctcgaagcg ctcgccgccg cggcttccct ttcattcggc
     6541 tgagcgtttc cggtttcaga cgtttgagcg gtttgcgttt cgacaggctg aacgctttcg
     6601 gattcaaccg gataattttc cgcttcttcc ggctggatgc tttgagtctc gctcgcttca
     6661 gccgtttcgg cttcaaccgg ctcgaccgtc tgcatttcag tcgtttgaac gttttccgtt
     6721 tccgccgttt gataatcttc cgcttcggag acagccgtcc ggctttccgc gcccgcttca
     6781 ttcgggctcg cggtttccgc tacgggctga acactttccg tttcttcttc gtcctcgtct
     6841 tcatattcgt cgtcgtcgaa gagttccaga aaatcgtcgt cgtcgtcgga ttcaacgcgc
     6901 gaagcgggtt gtgatgattg agaaatttcg ctcgaagccg cctgctccga ttgcggcgtc
     6961 gattcttcct gtgccggaga atcataagcg tccggcgcgg cttcaatctc tcttaaatcg
     7021 gcggcggaaa gcggctgggt tttgccgtaa tcttcttctc gcggctcgac ataatttcgc
     7081 gacgcaaatt tctgcgactc gtcaaacgca aagctgctcg gctgattggt ttgaatcatt
     7141 tcgtcgtcga atccggcgtc ttcgtaacgc ggcgtttcgg attcgattac ttcggcttga
     7201 atcggcggct ccatttcccg cgtctcggca aagctgtttt ccggagcggt cggcgtcgct
     7261 tcgacgtttt ccgtctccga gcgctcgagc aactccgaaa ctttggtgac cagctggcga
     7321 atcgactgaa aaggcttggt taaataatcg tccgcgccga cgcggcgcgc ttcctgctcg
     7381 ttaaacgtct caaacgagcc gatcagcaaa atcaccggcg tatttttgaa tttttcgtgc
     7441 tgcttgattc gctcgcaaac ctcataaccg ctgtagcccg gcatatggac gtcagccatt
     7501 accaagtccg gcgaaatttc caaaagccgg tctatcgcct cgttgccgtt gctgatggga
     7561 aaaacatcga tgccttcatc ggcaaaggtc agattaacga ccttttgaat cgtcaccgaa
     7621 tcgtctgcca acaatatttt gcgtcttgcc actgagaatt ttgctccttg gtcttcaagc
     7681 cagccagctg ccgtttaaaa aaaatgctac gttttgaaag ctatcatcgc cagccgttta
     7741 cgtcaagaag attttgcgtt tccttaagcg ctgttgcggc ttttttgcac aaacatcttg
     7801 agcattgttt gcagctgaac cgtcgtaaac ggtttcggca aaaaagcgac cgcgcctgcc
     7861 gcaaaactgt ccgacgaaag acgcggattc tgctccgccg tcatcactat aaccggaatt
     7921 ttcatcaggc gtttttccga ctgcatataa cgaaccagct cggtgccctg aatataaggc
     7981 atcaccacgt cgaaaatcgc cgccacaaac ggctcgcccg attgcagaag tttataagcg
     8041 tcgcgcccgt ctcgcgccgt tatcaccgta taaccttctt tttcgacaat cgctttgaca
     8101 agacgtaaga ttgccgggtc gtcgtcggca actaagactt ggggaagtcc gtcctgtgtt
     8161 tcttcattca ttttgtattt taaacccttt tacgtttggt attttatatt aacaaaaaat
     8221 ttcttattca tctgaattaa accacaactt tttatgatta acgaacgaaa atcggcaaat
     8281 tcaaatgaaa cgcggctaca tactttccgc cgcccgattt ttcgtctgtt cttcgctcaa
     8341 atgcgccgcc agatttatcg cggctttcat actggacgcc tcggcgatgt tttttccggc
     8401 aatgtcaatc gccgtgccgt ggtcgaccga agttctgatt aaaggcaatc ccagcgtgac
     8461 gttgaccgac gcgccgaacg agagacattt gaccgcaatc gtcgcctggt cgtgataaca
     8521 ggcgacgacg gcgtcgaatt cgcctttgta gccgcgcaga aaaatcgtat ccggcgaata
     8581 cgctcccgat acgtcgacgc cgtgtttttc gcggcattcg ctgattgccg gagcgatttc
     8641 atcggcttct tccgcgccga acattccgcc ttccgaagcg tgcgggttca aaccggcgac
     8701 ggcgattttc acatcccttt tcagaagctt ttccagctcc cggttggtaa aaagtattaa
     8761 acgcaccaga ttttcttttt ttaccaaatt tattgcctcg accagcgaga cgtgcgtcga
     8821 aagcagaacg acgcgcagtt tttcggcaaa aaaagacatc gcaaaatctt tcgtgttcgt
     8881 cagcgcagct aaaaattcgg tgtgaccggg aaaatcgtac ccgccgagcg cgagcgcctt
     8941 tttgctgatc ggcgcggtcg ctatcgcatc aatccggttt tctttccaca gggcgaccgc
     9001 cgtttcgata tattccgccg aagcgcgtcc cgtcgcggct gattcttcgc cgggcgttat
     9061 cttttcattc aaattcgcta aatcgtaaat tactggtttg ccggagttta cgggaatgcg
     9121 cgcgcctttt cggacgacgg caaaatcatc gaaaagtccg aatttacgag cagtttcgag
     9181 caaaaatttc gcgtcgccga ttattacggg ctggcaggcg gctctgatgc gcgcgtcgga
     9241 aatcgctttc agcgtaattt ccgcgccgat gcccgccgca tcgcctatcg taacggcgac
     9301 gcgctgcgga tttttattgg tggatgaagt ttcggagaga ttttcttttg aggaagtttt
     9361 cacctggttg ataaacgagc ttcgggctcg ctacttgcgg tcctccgctt tgaacatata
     9421 tttgatgttg tgttttaaaa gcagaatatt cggctggtct ttacggttta tttttaaagc
     9481 gtcgcggtcg taccattcga ttgtgccctc gacctcctcg ccgtcctgaa gcacgacgac
     9541 catcgtcgta tgcgaatcaa tctgcttttt gtaatagtaa acttcggcat tggtttcggc
     9601 tggcggcggc atttttttgc ggtcatcagc cggggcggtc ggacgcgaaa aagacggcat
     9661 attatttttt cccctaatat tcattacttt tatcaaaatt ctcccgctta agttccggaa
     9721 gaatcgattt gtcaaatcaa ttttccgttt attacagtct ttgcaataaa cgaaagaatc
     9781 gcttttcaaa tcttttcgat ttcgatttta gtataagttt
//
LOCUS       DASWNE010000005         5358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_268, whole genome shotgun sequence.
ACCESSION   DASWNE010000005 DASWNE010000000
VERSION     DASWNE010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5358
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VGB68_00150"
     CDS             <1..286
                     /locus_tag="VGB68_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047664.1"
                     /translation="IETSDNIVTNDANHSGVFTINVQSGATQAVAESGSISMKNAVPP
                     TPQQTPTDDDDDDNSILVPVLVFAGIVGAAAVYTFTRDSDDLQFIVSPNR"
     gene            504..2108
                     /locus_tag="VGB68_00155"
     CDS             504..2108
                     /locus_tag="VGB68_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047665.1"
                     /translation="MSIREEQFNLTEIRRRFPLLVENLAHLANREAWLERIWEIEQRW
                     NKTRQNGSTDREVFSTGDAPKDLNAEAEFEIIYAGGTLGLLHAAVMAGGHNRKVLVFD
                     AHTVGKTHRDWNISDEELQEFTRAGLFTPEEIERAVANRYKTGFVKFYEANSRIKTPP
                     LFMENVLDVAIEADKLLDLAVKKLKQTDSKIINNLRFVRAYVQKEKVLVECEDVKTGK
                     RRLFSARLFVDATGTNSPVSRQLNAGKSITHVCPTVGTVAKGFQKGSAPNEVDFSVGE
                     ILVSTEDISDNRQLMWEGFAGNPRKNEYTTYLFFYDSVESKADKSLLRLFEEYFEKLP
                     AYKTKTNGWKIVKPVFGYIPSIHHHGWNNVKKTASDRILLIGDAAGLSSPLTFCGFGS
                     HVRNLRKLTNLTETALKNNALDEKSLSAINAYEPRVAQMASLAEFMRPVKTSKPSAVN
                     ETMNAVIMALSQLDEGVRREMFQDRLSFANFGRVLRKTATIHPKVFRLMFEHLGAKGS
                     FWWLANIAEAALHERKFPDNGKINRG"
     gene            complement(2159..2632)
                     /locus_tag="VGB68_00160"
     CDS             complement(2159..2632)
                     /locus_tag="VGB68_00160"
                     /inference="COORDINATES: protein motif:HMM:NF024106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative molybdenum carrier protein"
                     /protein_id="HEY0047666.1"
                     /translation="MLKIRRIISGAQTGVDRAAFDFALENGIETGGFVPKNRAAEDGR
                     ISEKYPNLIETETENPSERTGLNVISADATLIFSRGKLAGGSKLTKELAEKHRKPFLH
                     VDFSELTIRQAIEKTKIWLDSLDCESLNIAGARASEDAEIYGKTKEFLKELLKRE"
     gene            complement(2733..3257)
                     /locus_tag="VGB68_00165"
     CDS             complement(2733..3257)
                     /locus_tag="VGB68_00165"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HEY0047667.1"
                     /translation="MSEIEPVKTKSKKPTRKEKVQLKSRMSNLMTFLPNLVRLLGRLL
                     TDGAVPTAEKALFAAAIIYVIVPLDFIPDVFPFIGQVDDIYLVALTLLRLINRSDESI
                     VRRHWSGGGDIVGLADSIAGIAPMLLPKRVSRVLSAEVELADAGKALQNITKKRKAVV
                     AEIPLEEETPKIRS"
     gene            3555..4559
                     /gene="pta"
                     /locus_tag="VGB68_00170"
     CDS             3555..4559
                     /gene="pta"
                     /locus_tag="VGB68_00170"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEY0047668.1"
                     /translation="MILEKIRQRAAADLQHIVLPEGEDARTVRAAEMCVRDRVAKVTL
                     VGDEEKIRETARASSANLNGVDILDHRKSSDFGKIAQFYHELRRAKGVTLEESEQMVK
                     DPLFYGNLLVKLGEADGSVAGATNTTAHTVRAALHCIGVRQGFKIVSSFFVMVVPEKK
                     FGHEGAMIYADCGVVIDPDAAELAEIAIASADSCRALLGVEPRVAMLSFSTKGSASHK
                     LIDKVVEATKTVRARFPNLEIDGELQADAALVPTVAASKAQGSSVAGKANVLIFPDLN
                     AGNIAYKLTERLTGGAAIGPILQGLDKPANDLSRGCKAEDIVDAVAITAVQSQARKVQ
                     "
     gene            complement(4897..5343)
                     /locus_tag="VGB68_00175"
     CDS             complement(4897..5343)
                     /locus_tag="VGB68_00175"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY0047669.1"
                     /translation="MTDEEWHKMRLHPIYGQRILRNIPFLEGASRVVSQHHEKWDGTG
                     YPLKLRGEEIDLGARIFAVVDAFDAMISNRIYRRGRSFEAALQELERCAGTQFDPQII
                     EAFKNVPKEDWETLRERSLKDRAEISSFQAVVAELVYSKHQFELVH"
BASE COUNT         1348 a         1410 c         1354 g         1246 t
ORIGIN      
        1 tatcgaaacc agcgacaaca tcgtgaccaa cgacgccaat cacagcggcg tttttacaat
       61 caacgtgcaa tccggcgcga cgcaggcggt tgccgaaagc ggctcgattt ctatgaaaaa
      121 cgcggtgccg ccgaccccgc agcaaactcc gaccgacgac gatgacgacg acaacagcat
      181 cctcgttccg gtgctggttt ttgccggaat cgtcggcgcg gcggctgtct acacgtttac
      241 gagagacagc gacgatttgc agtttatcgt cagcccgaat cgttaataac tcttaaatag
      301 atagttaaaa aggcacgaac gttcaagttc gtgccttttt tattcggtaa agcaaaacgc
      361 cgattgcctg aaccggaaag caaacttaaa atagtaaaaa agtgagcggc gagcggcgag
      421 cagttttaaa tccgagattg taatgttgaa attcaaaatc tcactgctcg ctgtttaccg
      481 cttactgctc actccaaaaa acaatgagca ttcgggaaga acaattcaat ctcacggaaa
      541 ttcgccgccg gtttcctctg ctggtcgaaa atctcgcgca tctggcaaat cgcgaagcgt
      601 ggctcgaaag aatctgggaa atcgaacagc gctggaacaa gacgcgccaa aacggctcga
      661 cggacaggga agttttttcg accggcgacg cgccgaaaga tttaaacgcg gaagccgaat
      721 ttgaaatcat ctacgcgggc ggaacgctcg gactgctgca cgcggcggta atggctggcg
      781 ggcacaatcg aaaagtcctg gttttcgacg cgcacaccgt cggcaaaacg catcgcgact
      841 ggaacatctc cgacgaagaa ttgcaggaat ttacccgagc cggacttttc acgcccgaag
      901 aaatcgaacg ggcggtcgcc aatcgctata aaaccggttt cgtaaaattt tacgaggcga
      961 attccagaat aaaaacgccg ccgctgttta tggaaaatgt tttggacgta gcgattgaag
     1021 ccgataagct gctcgattta gccgtcaaaa aactcaaaca aaccgattcg aaaatcatca
     1081 acaatctgcg cttcgtgcgc gcctacgtgc aaaaagaaaa agttttggtc gaatgcgaag
     1141 acgtgaagac cggaaagcga cgtttgtttt ccgcaaggct tttcgtcgat gctacgggga
     1201 cgaattcgcc cgtctccaga cagttgaatg cgggaaaatc aatcacgcac gtgtgcccga
     1261 cggtcggcac cgtagccaaa ggttttcaga agggaagcgc gccgaacgag gtcgattttt
     1321 cggtcggcga aattctggtc tcgaccgaag acatttccga taatcgacaa ttgatgtggg
     1381 aaggatttgc cggaaatccg cgaaaaaacg aatacacgac ttatcttttc ttttacgatt
     1441 cggtcgaatc aaaagccgac aaatctttgc ttcgtttgtt tgaggaatat tttgaaaaac
     1501 tgcccgctta caaaacgaaa accaacggct ggaaaatcgt caagcccgtt ttcggctata
     1561 ttcccagcat tcaccatcac ggctggaaca acgtcaaaaa aacggcgtcc gacagaattc
     1621 tgctcatcgg cgacgcggcg ggtttgtcga gtccgctgac gttttgcggc ttcggctcgc
     1681 acgtgcgcaa tttgcgcaag ctgacgaatc tgacggaaac ggctttgaaa aacaacgcgc
     1741 tggacgaaaa atctctgtcc gccatcaacg cttacgagcc gcgcgtggcg cagatggcga
     1801 gcctcgccga atttatgcgc ccggtgaaaa ccagcaaacc gtcggcggtc aatgaaacga
     1861 tgaacgccgt cataatggct ctgagccagt tggacgaagg cgtccgccgc gagatgtttc
     1921 aggacaggct ttcattcgcc aattttggac gggttctgcg aaaaacggcg acgattcacc
     1981 cgaaagtttt tcgcctgatg ttcgagcatc tcggcgcaaa aggctcgttc tggtggctgg
     2041 caaacatcgc cgaagccgcg ctgcacgaaa gaaaatttcc ggataacggg aaaattaatc
     2101 gaggataacg gcgataaacg ggaattttaa taaattatct ccttcccgcc caaccggttc
     2161 attcccgttt caataattct tttaaaaact ctttcgtttt cccgtaaatc tccgcgtctt
     2221 ccgaagcccg cgcgcctgcg atattcaggc tttcacaatc gagcgaatcg agccagattt
     2281 tcgttttctc gattgcctgt ctaatcgtca gctcggaaaa atcgacgtgc agaaaaggtt
     2341 tccggtgttt ttcggcaagc tcttttgtaa gctttgagcc gcccgccagc ttgccgcgcg
     2401 aaaaaatcaa ggtcgcgtcg gcgctaatta cattcaaacc ggttctttcc gacggatttt
     2461 ccgtttcggt ttcaatcaga ttcggatatt tttcgctgat tctgccgtct tccgccgcgc
     2521 gatttttcgg cacgaagccg ccggtttcga ttccgttttc gagcgcgaaa tcaaaagctg
     2581 cgcggtcgac gcccgtctgc gcgccggaga ttatcctgcg aattttcaac ataaaaacta
     2641 aacgcggatt tacgcggata aacgcagatt tttgtttaat tttatctgtt tttatccgcg
     2701 taaatccgcg gtttcgctct ctttcgtttt aatcagctcc gaattttcgg agtttcttct
     2761 tcgagcggaa tttccgcgac gaccgctttg cgttttttcg tgatgttttg cagggctttg
     2821 ccggcgtcgg caagctcgac ctcggcggaa agcacgcgcg aaactctttt cggcagaagc
     2881 atcggcgcaa ttccggcaat cgaatccgcc aagccgacaa tgtcgccgcc gcccgaccag
     2941 tgccgccgca caatcgactc gtccgagcga ttaatcaggc gcagaagcgt cagcgcgacc
     3001 agataaatat cgtcaacctg cccgataaac gggaaaacgt cgggtataaa atcgagcggc
     3061 acgatgacgt aaataatcgc cgccgcaaac aaggcttttt cggcggtcgg aacagcgccg
     3121 tcggtcaaaa gcctgcccag cagacgcacc agattcggca gaaacgtcat cagattcgac
     3181 atacggcttt tcaactgcac tttttccttg cgcgtcggct ttttcgattt ggtctttaca
     3241 ggctcgattt cgctcattag atttattccc ctttttttat ttttattagt ctagaaatta
     3301 tcgggtcgtt tgtgcaagtt taatttagaa aagattttta acgcaaagtc gcaaaggagg
     3361 cgaggaaaaa atctgtgacc gtaaattttc gcggataaac gcaaaataaa ttttcaggga
     3421 tgaagaagat aaaggaagat aaaacaaaat cattaattat cctgtatttt tattcccggc
     3481 tcatccgcgc cgtcgctgta aattctttct tcgcctcctt gcacctttgc gcccttgcgt
     3541 taaaatcttt ttatatgatt ctcgaaaaaa tccgccagcg cgccgccgcc gatttgcagc
     3601 atatcgtttt gcccgaaggc gaagacgcgc gcaccgtccg cgctgccgaa atgtgcgtcc
     3661 gcgaccgcgt cgccaaagtg acgctcgtcg gcgatgagga aaaaatccgc gaaaccgccc
     3721 gcgcttcgag cgccaacctg aacggcgtcg atattctcga ccaccggaaa tcgtcggact
     3781 tcggtaaaat cgcgcagttt tatcacgagc tgcgacgggc gaaaggcgtc acgctggaag
     3841 aatccgagca gatggtcaaa gacccgcttt tctacggcaa cctgctcgtc aagctcggcg
     3901 aggctgacgg ctcggtcgcg ggcgcgacga atacgacggc gcacacggtt cgcgccgcgc
     3961 tgcactgcat cggcgtccgg cagggcttta aaatcgtttc cagctttttc gtgatggtcg
     4021 tcccggaaaa aaaattcgga cacgaaggcg cgatgattta tgccgactgc ggcgtggtga
     4081 ttgacccgga cgcggcggag ctggcggaaa tagctatcgc ctcggctgat tcgtgccgcg
     4141 cgcttttagg cgtcgagccg cgcgtcgcga tgctcagttt ttcaaccaaa ggcagcgcgt
     4201 cacacaagct gattgacaag gtcgtcgaag cgaccaaaac cgtcagggcg cgtttcccaa
     4261 acctcgaaat cgacggcgag ctgcaagccg acgccgccct cgttccaaca gtcgccgctt
     4321 caaaagcgca gggctcgagc gtcgcaggaa aagccaacgt cctgattttc cccgatttaa
     4381 acgccggcaa tatcgcctat aaattaaccg aaagactaac cggcggcgcg gcaatcggac
     4441 ccatcctgca aggcttggac aaacccgcca acgacctctc gcgcggctgc aaagccgaag
     4501 acatcgtcga cgcggtggca atcacggcgg ttcagtcgca ggcgcggaag gttcagtaaa
     4561 atttaacggc ggagttgagg cggggcgaat tcttctaaga atcatcttac aacctttcgt
     4621 aaaaaccatc gaaaatcttt tttgccagcc cttgatttta cgatgcagcc ttcttcgttt
     4681 tgttgtcttg tcccgcttct tcttgataat tggttctatt ctttttgaac gagcgagcga
     4741 gagaagtttt agcatgattg gcgcgcgaaa gcagcggctt gagcgtcgct taaataataa
     4801 agtatgcgga atccttgcag aaaacaaaaa ttccgcatac gaccttcgta tgcggaacac
     4861 ttagacaata atttaaccgt gcgatgcaat aaaaatttaa tggactaatt cgaattgatg
     4921 ttttgaataa accagttccg cgacgaccgc ctgaaacgag gagatttccg cgcggtcttt
     4981 cagcgagcgc tcgcgcaggg tttcccagtc ttccttcggc acgtttttaa aagcttcgat
     5041 aatctgcggg tcgaactgcg ttccggcgca gcgctccaat tcctgcaaag ccgcttcgaa
     5101 cgagcgaccg cgacgataaa tgcgattgga aatcatcgcg tcgaaagcgt ccacgacggc
     5161 gaagatgcgc gcgccgaggt cgatttcctc gccgcgcagc tttaacgggt aaccggtgcc
     5221 gtcccatttt tcgtgatgct gcgagacgac gcgcgatgcg ccttcgagaa aaggaatatt
     5281 gcgcaaaatc ctttgaccgt aaatcgggtg cagccgcatt ttatgccatt cttcgtcggt
     5341 cagtttggcg ggctatcg
//
LOCUS       DASWNE010000006         5302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_353392, whole genome shotgun sequence.
ACCESSION   DASWNE010000006 DASWNE010000000
VERSION     DASWNE010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5302
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353392"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1379
                     /locus_tag="VGB68_00180"
     CDS             <1..1379
                     /locus_tag="VGB68_00180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEY0047670.1"
                     /translation="FKYLMAQGQTGLSTAYDLPALMGLDADSPLSEGEVGKCGVAVSS
                     FADFEVLFDGINLEDVTVSQTINAPAWIFLAFYVALAEKQGADLRKISGTLQNDILKE
                     YIAQKEWIYPIKPAMKLVIDTFEYCTKFVPKYNPISVSGYHIREAGATALQELAFTLR
                     DGVEYVQYGVERGLNVDEFVPRISFFFNAHNDFFEEIAKYRAARKVWAKTMRERFGAK
                     NPRSLQMRFHTQTAGVSLTVQQPLNNIARVAIQALAAVLGGTNSLHTDSYDEALALPT
                     DQAALIALRTQQIIAEETGVVNTVDPLGGSWYLESLTEKMEKGCLDYFDKIDGFGGMV
                     EAIEAGFPQREIQESAYQYQKAVERKEQTIVGVNKYTMADEFSKIDILQIDESVRDLQ
                     LNRLAHTKSTRDNGAVEIALDKLKKAAQANENTMPATIEAVKAYATVEEICSALRDVY
                     GIYEEPAF"
     gene            1461..1628
                     /locus_tag="VGB68_00185"
     CDS             1461..1628
                     /locus_tag="VGB68_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047671.1"
                     /translation="MARLNRIHGKGRWRKLKGIGNVELPSGRIRLAEVHWYEAHGIGK
                     KEMKIKLPFLD"
     gene            1631..1849
                     /locus_tag="VGB68_00190"
     CDS             1631..1849
                     /locus_tag="VGB68_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047672.1"
                     /translation="MEEHAFVVCINNKGYEASLEFGKIYQVVIDKEAEAEGLIRIIDE
                     SGEDYGYSLERFHSVVLPQTLENVLRAA"
     gene            2093..2908
                     /locus_tag="VGB68_00195"
     CDS             2093..2908
                     /locus_tag="VGB68_00195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEY0047673.1"
                     /translation="MLKPTVIAIPIFALLIAVEAYIAVRHAREDYDRKDAWTNIALGF
                     GSVAFGAVFGLIQAFFYDAIYENLAPYRMPMNAWWAWALLLFVDDFAYYWFHRVSHEM
                     RFFWNFHVVHHSSNQYNLSVAVRQSWFSGIAHWLFYVPIAFLGFPLWSFVLMHGLNLI
                     YQFWIHTKLIKSLGFLEYFLNTPSHHRVHHGVNDAYLDKNYGGIFIIWDRLFGSFAKE
                     DETPRYGIIKPIHSRNWLWINTHAWAEMLTAMKKQKTILGKIRCVFGSPNMEF"
     gene            2916..3659
                     /locus_tag="VGB68_00200"
     CDS             2916..3659
                     /locus_tag="VGB68_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047674.1"
                     /translation="MKTGIFAFSLIFLFAAQAFSQIQNVKITPRKIVFRRADTSVDYK
                     KRYTIVYPKISGVDRRLKRKIERNLKYEKTFNFYFTSGLDEQYHLLDKAAYRVVYNKN
                     GILNLRFSIDVSTAYKSRYDKTVAVNLKSGEIIKPEDVFIEARQQQLAARINKTLQAE
                     IAGAVRRVKKESGGDEGGQIPAQFREKIFTPLYLDNFEVSDKGITFVYTYDYAHIAGN
                     YKPPGRFFLSFAQLKPFVRRDGLFGRFVR"
     gene            complement(3662..3919)
                     /locus_tag="VGB68_00205"
     CDS             complement(3662..3919)
                     /locus_tag="VGB68_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047675.1"
                     /translation="MPEILSVLFALLILVALFKLFFDDLEDLWTSVKSCCCWFGFSIV
                     SERWAIFEWRSPKTEQTGSRLFFWLAIGALGGFMLYHNLSK"
     gene            4042..4446
                     /locus_tag="VGB68_00210"
     CDS             4042..4446
                     /locus_tag="VGB68_00210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEY0047676.1"
                     /translation="MSERKIRVLVAKPGLDGHDRGAKVIARALRDAGMEVIYTGLRQT
                     PEMIASAALQEDVDAVGISILSGAHNTLCPRIVNLLRENGMDDTLVLVGGIVPQEDIS
                     ELKAKGISEVFLPGTSTEDIVKFLRENVKNKN"
     gene            4558..4857
                     /locus_tag="VGB68_00215"
     CDS             4558..4857
                     /locus_tag="VGB68_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047677.1"
                     /translation="MKKITSLIFAAAMILSIAFVGEMTSSNNPFSADAQVTVVRKKRV
                     GAIRTVGRGGKYVVRKTWNGTKYVSKKVWSGTKWTGKKTYKTGRKVVSRTKKVVY"
     gene            5044..>5302
                     /locus_tag="VGB68_00220"
     CDS             5044..>5302
                     /locus_tag="VGB68_00220"
                     /inference="COORDINATES: protein motif:HMM:NF018938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEY0047678.1"
                     /translation="MKQKVRVASGQGFWGDLLTAPVEQVRGGEIDYLMLDYLAEVTMS
                     IVQKQKQRDPKAGYARDFISLMREILPDCVEKNIKVTANAGG"
BASE COUNT         1442 a         1234 c         1357 g         1269 t
ORIGIN      
        1 gattcaaata tttgatggcg cagggacaaa ccgggctttc gaccgcgtat gatttgcccg
       61 cgctgatggg cttggacgcg gattccccgc tttcggaagg cgaagtcggc aaatgcggcg
      121 tcgcggtttc gtcgttcgct gatttcgagg ttttattcga cggaataaat ctcgaagatg
      181 taacagtttc gcagacgatt aacgcgcccg cgtggatttt tttggcgttt tacgtcgcgc
      241 tcgccgaaaa acagggcgcg gatttgcgta agatttccgg cacgttgcag aacgatattt
      301 tgaaggaata tatcgcgcag aaagaatgga tttacccgat aaaaccggcg atgaagctgg
      361 tcatcgacac gtttgaatac tgcacgaaat tcgtgccgaa atacaacccg atttccgtct
      421 ccggctatca tatccgcgag gcgggcgcga ccgctttgca ggaactcgct ttcaccttgc
      481 gcgacggcgt cgaatacgtc cagtacggcg tggaaagagg cttaaacgtc gacgaattcg
      541 tgccgcgaat ctcgtttttc tttaacgcgc acaacgactt tttcgaggaa atcgccaaat
      601 atcgcgccgc gcgaaaagtc tgggcgaaaa cgatgcgcga acgcttcggc gcaaaaaatc
      661 cgcgctcgct ccagatgcgt tttcacacgc agacggcggg cgtttccctg accgtccagc
      721 agcctttaaa caacatcgcg cgcgtcgcca ttcaggcgct ggcggcggtt ctcggcggaa
      781 cgaactcgct gcacacggat tcttacgacg aggcgctggc tctgccgacc gatcaggcgg
      841 ctttgattgc tttgcggacg cagcagatta tcgccgaaga aaccggcgtc gtgaacacgg
      901 tcgacccgct cggcggtagc tggtatctgg aatctctgac cgagaagatg gaaaagggct
      961 gtctcgatta tttcgataaa atcgacggtt tcggcggtat ggtcgaagcc atcgaagccg
     1021 gatttccgca gcgcgagatt caggaatcgg cgtaccaata tcaaaaggcg gtcgagcgca
     1081 aagagcagac catcgtcggc gtcaacaaat acacgatggc tgacgagttt tccaaaatcg
     1141 acatcctgca aatcgacgaa tcggtgcgcg acctgcaatt aaatcgcctc gcgcacacaa
     1201 aatcgacgcg cgacaacggc gcggtcgaaa tcgctctcga caaactgaaa aaagccgcgc
     1261 aagcgaacga aaacacgatg cccgcgacga ttgaagccgt caaagcttac gccacggtcg
     1321 aagaaatctg ctcggctctg cgcgacgttt acggcattta cgaggaaccg gctttttaat
     1381 tgacatgatt gctagacttt aaattagtcg gcaagattac gaatattgaa acaattgcaa
     1441 ttggaaacag tattcgtgat ttggcgcgtt tgaaccgaat tcacggcaaa ggtcgttggc
     1501 gaaagttgaa aggaatcggc aatgtcgaac ttccgagcgg cagaattcgc ttggcggaag
     1561 tgcattggta tgaagcgcat ggaatcggca aaaaagagat gaaaattaaa ttgccgtttt
     1621 tggattagct atggaagaac acgcatttgt agtttgcatc aataataaag gttacgaagc
     1681 gtcgctcgaa ttcggcaaaa tttatcaggt tgtgattgat aaagaagcgg aagccgaagg
     1741 tttgattcgc attattgacg aaagcggcga agattacggt tattcgcttg agagatttca
     1801 ttcggtcgtt ttaccgcaaa ctctggaaaa tgttctgcgc gcagcttgag tatcgaagaa
     1861 atccgctccg ccctgcgcga cgtttacggc atttacgagg aaccggcgtt ttaggagttt
     1921 tggattttgc gagcaaatat ttaaagatac gataatttct ttcaaatgtt ttggcgaaaa
     1981 acaatcggca aaccacgctg agtttgattg aatcttaatt ttgggattgt gaaacttgat
     2041 ttattatcac aatcgacgag attatttttc caaaaccaat taagtttttt tgatgttgaa
     2101 accgaccgtt atcgccattc cgatattcgc gctgctgatt gcggtcgagg cttatattgc
     2161 cgtgcgtcac gcgcgggaag attacgaccg caaggacgct tggacgaaca tcgcgctcgg
     2221 gttcggcagc gtcgctttcg gagcggtttt cggtttgata caggcgtttt tctacgacgc
     2281 gatttatgaa aatctcgcgc cgtaccggat gccgatgaac gcctggtggg cttgggcgct
     2341 tctgcttttt gtcgacgatt tcgcttatta ctggtttcat cgcgtcagcc acgagatgcg
     2401 ttttttctgg aattttcacg tcgttcatca ttcgtcgaat caatacaatc tgagcgtcgc
     2461 cgtgcggcaa agctggtttt ccggcatagc gcattggctt ttctacgtgc cgattgcttt
     2521 cctgggcttt ccgctctggt cgttcgttct gatgcacggg ctgaacctga tttatcaatt
     2581 ctggattcac accaagctga ttaaaagcct cggctttctc gaatattttt taaacacgcc
     2641 ttcgcatcac cgcgttcatc acggcgtcaa cgacgcttat ctggacaaaa actacggcgg
     2701 aatttttatc atctgggaca ggcttttcgg aagtttcgcg aaagaagacg aaacgccgcg
     2761 ctacggcatc atcaaaccga ttcacagccg caactggctc tggataaaca cgcacgcgtg
     2821 ggcggagatg ctaacggcga tgaaaaaaca aaaaactatt ttaggcaaaa tcaggtgcgt
     2881 tttcgggtct ccaaatatgg aattttgaga ctactatgaa aaccgggatt tttgctttct
     2941 cgttaatttt tcttttcgcc gcgcaagcgt tcagccagat tcagaacgta aaaatcacgc
     3001 cgcgcaaaat cgtcttcagg cgcgccgata cttccgtcga ttataaaaag agatatacaa
     3061 tcgtctatcc gaaaatttcc ggcgtcgacc gccgtctgaa acggaaaatc gagcgtaatc
     3121 tgaaatacga aaaaacgttc aatttctatt ttacaagcgg cttggacgag caataccatt
     3181 tgctcgacaa agccgcttat cgagtcgtct acaacaaaaa cggcattttg aatttgagat
     3241 tttccatcga cgtttcgacg gcttacaaat cgcgttacga taaaacagtc gccgtcaatc
     3301 tgaaatcggg cgagataatc aaaccggaag acgtttttat cgaagcgaga cagcaacaac
     3361 tggcggcaag aatcaacaag actctgcagg cggaaatcgc cggagcggtg cgccgtgtga
     3421 aaaaagaatc gggcggcgac gaaggcgggc agattccggc gcagttcaga gaaaaaattt
     3481 ttacgcccct gtatctggac aatttcgaag tgagcgataa aggaattact ttcgtttaca
     3541 cctatgacta cgcgcacatt gccggaaact acaagccgcc cggcagattt ttcctgagct
     3601 tcgcgcaatt aaaaccgttc gttcgccgcg acggcttgtt tggaagattc gttcgctaaa
     3661 gctattttga aaggttgtgg taaagcataa agccgccgag cgcgccgata gcgagccaga
     3721 aaaaaagtct cgatcccgtt tgctcggttt tgggcgaacg ccactcgaat attgcccagc
     3781 gctcggacac gatgctgaaa ccgaaccagc agcaacagct tttgacgctc gtccacaaat
     3841 cttccaaatc atcgaaaaat aatttaaaaa gcgcgaccag aatcagcaag gcaaataata
     3901 cggaaagtat ttcgggcata tttgaaagcg ggctttgaag cggcgggaac gactcgcatt
     3961 tataagataa cagaaaaaga ttttattttg aaagcatttt ttgaattgaa aactttaaga
     4021 agttgcgatt aagataaacc tatgagcgaa agaaaaatac gagttctcgt ggcaaagccc
     4081 ggactggacg ggcacgaccg cggcgcgaaa gtcatcgcgc gcgcactgcg cgatgccgga
     4141 atggaagtta tttacacagg cttgaggcaa acgccggaaa tgattgcgtc agccgccctg
     4201 caggaagacg tcgatgcggt cggcatctcg attctgtcgg gcgcgcacaa tacgctttgc
     4261 ccgcgcatcg tcaatcttct gcgcgaaaac ggaatggacg atacgctggt tctggtcggc
     4321 ggaatcgtgc cgcaggaaga tatttccgaa ctgaaagcga aaggaatttc cgaagtcttt
     4381 ctgcccggca cttcgaccga ggacatcgtg aaatttttgc gcgaaaacgt caaaaataaa
     4441 aattaatttc tgtggaataa tgtttgcact taacgtcttc aaacgccgaa cagtgtatta
     4501 taaatttaca caaaccggcg gaattagttt ttaataaata caacaaggag attcgagatg
     4561 aaaaaaatta cgtctttgat atttgctgcg gcaatgattt tatcaatcgc ttttgtcggc
     4621 gagatgactt cttccaataa tccgttttca gccgacgcgc aggtaacggt cgtccgcaag
     4681 aaaagagtgg gcgccatcag aacagtcgga cgcggcggca aatacgtcgt tcgcaaaacc
     4741 tggaacggca cgaaatacgt cagcaaaaaa gtctggagcg gcacgaaatg gacgggcaaa
     4801 aagacttata aaaccggcag aaaagttgtc agccgaacga aaaaagtcgt ttactagttt
     4861 ctgattttca gaacggcaac cgtcggggcg gtggcaaatc agcaaaggca tctttgacga
     4921 tttgcgaccg cctcgtatta taatttctta atgattgcga attcaggatt caggattcag
     4981 gattggtcgt tgaataacgc aaatcctgaa tcctgaatcc tgaatcctga atcctaaact
     5041 acaatgaaac aaaaagtaag agttgccagc ggacagggct tttggggcga tttgctgacc
     5101 gcgccggtcg agcaggtgcg cggcggcgag attgattatc tgatgctcga ttatctggcg
     5161 gaagtcacca tgagcatcgt gcagaagcag aaacagcgcg acccgaaagc cggttacgcg
     5221 cgcgatttca tcagtctgat gcgcgagatt ctgcccgatt gcgtcgagaa aaacataaaa
     5281 gtcacggcaa acgcgggcgg cg
//
LOCUS       DASWNE010000007         1850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_459695, whole genome shotgun sequence.
ACCESSION   DASWNE010000007 DASWNE010000000
VERSION     DASWNE010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459695"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VGB68_00225"
     CDS             <1..311
                     /locus_tag="VGB68_00225"
                     /inference="COORDINATES: protein motif:HMM:NF014671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEY0047679.1"
                     /translation="GNLVGLREDVGRHNAVDKLIGARVLSNEIPLADKILFLSGRASF
                     ELVQKAVMAQIPVMVAVGAPSSLAVEAAKKFGITLLGFVRRNRFNVYAGLERINFEAE
                     "
     gene            315..>1850
                     /locus_tag="VGB68_00230"
     CDS             315..>1850
                     /locus_tag="VGB68_00230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HEY0047680.1"
                     /translation="MRTEHGRANAEFSEELGDLEVGELKNTAAGINAITDTLKNTLGK
                     MGVVRGTRALLKLNQKGGIDCQSCAWADPEEHRTYAEFCENGAKAMADEGTTKRVTPD
                     FFAGYSVAELSEKSDQWLNAQGRLTQPMILREGATHYEPISWENAFQTLADEMNSLAA
                     PDEAVFYTSGRTSNEAAFLYQLFARQFGTNNLPDCSNMCHESTSYALNESIGLGKASI
                     RLEDLENTELIIIIGQNPGTNAPRMMSSLQEAKRNGARIIAVNPMPEAGLMNFINPNP
                     QHYPNPLMFPVDVLGNRSSQLADLHLPLRIGGDMAVLKGMMKVLLEMEKQNPGAVFDH
                     KFIAEKTSGFDEFIENLEKIPWSEILEQSGLTRGQIDEAARMYASANRVITCWAMGVT
                     QHKHAVDTIQDIASLHLLRGNIGRQGAGLCPVRGHSNVQGDRSMGIWEKMRDDFRANL
                     EEEFNFKTPEKDGFDTVEAIKAMHAGQAKVFFAMGGNFLSATPDTHCTAEALRNCNLT
                     AQVI"
BASE COUNT          468 a          486 c          525 g          371 t
ORIGIN      
        1 ttggaaattt agtcgggctg cgcgaggatg tcgggcgaca caacgcggtc gataaattaa
       61 tcggcgcgcg agttttatca aacgaaattc cgcttgcgga taaaattctc tttttgagcg
      121 ggcgggcaag cttcgagctc gtgcaaaaag ccgtgatggc gcagattccg gtaatggtgg
      181 cggtcggcgc gccgtccagc ctcgccgtcg aagccgcaaa aaaattcggc ataacgctgc
      241 tcggtttcgt gcgccgcaat cgttttaatg tttacgcggg attggaaaga atcaattttg
      301 aagctgaata aaatatgaga accgaacacg gacgggcaaa cgctgaattt tcggaagagc
      361 tcggcgattt ggaagtcggc gaattaaaaa atacggcggc gggaatcaac gcgattaccg
      421 acacgctgaa aaatactttg gggaaaatgg gcgtcgtgcg cggcacgcgc gctttgctca
      481 agctcaatca aaaaggcgga atcgactgtc agtcctgcgc ctgggctgac ccggaagaac
      541 atcgcacgta cgccgaattc tgcgaaaacg gagcgaaagc gatggcggac gaaggcacca
      601 cgaaacgcgt cacgccggat ttttttgccg gctacagcgt cgccgaattg tcagaaaaat
      661 ccgaccagtg gctcaacgcg cagggacggc tcacgcagcc gatgattttg cgcgaaggcg
      721 cgacgcacta cgagccgatt tcgtgggaaa acgctttcca aacgctcgcc gacgagatga
      781 attctctcgc cgcgccggac gaagccgttt tttacacttc gggcagaacc tcgaacgaag
      841 cggcgtttct atatcaatta ttcgcccgcc agttcggcac aaacaatctg ccggactgct
      901 cgaatatgtg ccacgaatcg accagttacg ccctcaacga atcaatcgga ctcggcaagg
      961 cgagcattcg tctggaagat ttggaaaaca cggagctaat catcatcatc gggcagaatc
     1021 cgggcacgaa cgcgccgcgg atgatgtctt cattgcagga ggcaaagcgc aacggcgcgc
     1081 gcattatcgc cgtcaacccg atgcccgaag ccggattgat gaattttatc aatccgaatc
     1141 cgcagcatta tccgaacccg ctgatgtttc ccgtcgacgt tttgggaaac cgctcgtcac
     1201 agctcgccga tttgcatctg ccgcttcgca tcggcggcga tatggcggtt ttaaaaggaa
     1261 tgatgaaggt tttgctggaa atggaaaagc aaaatccggg cgcggttttc gaccataagt
     1321 ttatcgccga aaaaacttcc ggcttcgatg aattcatcga aaatctggaa aaaattccct
     1381 ggtcggagat tctggaacaa agcggtctga cgcgcgggca aatcgacgaa gccgcccgga
     1441 tgtacgccag cgccaatcgc gtcattacct gctgggcgat gggcgtcacg cagcacaaac
     1501 acgccgtcga tacgattcag gacatcgcca gcctgcatct tctgcgcgga aatatcggca
     1561 ggcagggcgc gggcttgtgt cccgtgcgcg gtcattccaa tgtgcagggc gaccgctcga
     1621 tgggcatctg ggaaaagatg cgcgacgatt ttcgcgccaa tctcgaagag gagtttaatt
     1681 ttaaaacgcc ggaaaaagac ggtttcgata cggtcgaagc catcaaagcg atgcacgccg
     1741 gacaagccaa agtctttttc gcgatgggcg gaaattttct gagcgcgacg cccgacacgc
     1801 actgcacggc tgaagcgctg cgcaactgta atctgacggc gcaggtcatc
//
LOCUS       DASWNE010000008         4895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_71114, whole genome shotgun sequence.
ACCESSION   DASWNE010000008 DASWNE010000000
VERSION     DASWNE010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71114"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1939
                     /locus_tag="VGB68_00235"
     CDS             <1..1939
                     /locus_tag="VGB68_00235"
                     /inference="COORDINATES: protein motif:HMM:NF019239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0047681.1"
                     /translation="VLLLVLAFSNVNAAAKDEWLAVRSKNFYLIGNASDKEIRKVATQ
                     LEQFREVFRQILNKVNFNSPIPTTVIVFKDDKAYNPFKPVRATGKINKNIAGYFMPGS
                     DVNYITLAIQSDMSEVYGTIFHEYTHFLVNNNIGKSNVPPWFNEGLAEYYQTFKIEDD
                     QKVTLGSLQNGHLYSLQQNKLIPFDTFFNIDNYSLHQQGDHGTNMFYAQSWALMHYLI
                     HGEGGARSKQMNKFLDLVMNGRQAREAFSEAFQTDYATMEKELKKYVEKSSYKVTGIV
                     FKNKLTFDTEMRTAPLTQPEGQAYLGDLLYHMNRLGDAETLLQQVLTLNPDSSLAQTS
                     LGLVKMRQKNFDEAKKYLEKAVQSDSSNYLAHYSYAYVLSREAMNGGNFASGLEPESA
                     DKMRQALKKAIALNAGFAESYDLFAFISVVRNENLDEAVLYMKKALQIAPGNQQYMIR
                     AAELLMRKKEFAEAKVIAQKVVETADEPQLKSYAQSVLSSINSYERGLEEMKKYNERG
                     GGIISSTTTIADDKPMSEEEIAKTRAEMQLKSLNNILRKPNADEKRVLGYLTKIDCTA
                     KGMTYTVKVDGQLMKFTSTSFDTVEMITVNVNLEDGQINCGALKKETFALVTYRAGKD
                     AKTAGEIVAFEFVPEAFKLLN"
     gene            complement(1941..2543)
                     /locus_tag="VGB68_00240"
     CDS             complement(1941..2543)
                     /locus_tag="VGB68_00240"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEY0047682.1"
                     /translation="MPTKNNDLHGNAPENSPIVLLIIDVISDFDFPDGDKLLENTKPI
                     VKNIAALRDKADKAKIPVIYINDNFGKWQSDFKKLLKHASAKTSKGHAVAKQLKPKAK
                     DYFVLKPKHSGFYSTTLDLLLEYLNAKTLILTGLTADICVLFTANDAYLRDFKIIVPR
                     DCVAATEAEEKEKALKYIERVLHADTRPSSEIQFGKTGIK"
     gene            complement(2685..2903)
                     /locus_tag="VGB68_00245"
     CDS             complement(2685..2903)
                     /locus_tag="VGB68_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047683.1"
                     /translation="MELTKSKRYERKQTQRKKNAFQKVILEGVLKITSIQKAIHYRFG
                     AFANFPANALYTRYKIRPNHHFQNYLAD"
     gene            3437..3871
                     /locus_tag="VGB68_00250"
     CDS             3437..3871
                     /locus_tag="VGB68_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047684.1"
                     /translation="MNKIKAKHLATGDRVRFSRDGWQTVGRITNCNSISVSFMFGDGI
                     IRKLEANQEYVVEQAEQPPEPQPGQIMRLQKESNRVSVCASHSLFDLSDISEAFRAIK
                     QKDGYSNSSAAYHELERGAILHTHDAVYWMPLKWSGEATKEK"
     gene            4254..>4895
                     /locus_tag="VGB68_00255"
     CDS             4254..>4895
                     /locus_tag="VGB68_00255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-60 repeat domain-containing protein"
                     /protein_id="HEY0047685.1"
                     /translation="MLTTQAAAGALDPAFAGGGMTPSLGANFFSASSFAVQPDGKIIV
                     GGVYSLSTEEPFANAQFALVRYLPTGIIDSSFGTNGRVVTTFTEYPATAMSLNDIAVL
                     PDGKIVAVGSGRLAAETPHLTRLSLFVVRYNADGSLDTPFSNFGKFVSHAGGIQTTAQ
                     AVAVQPDGKFVVAGYAGGAINSLVPYKFILFRFSGNGPDPTFGNNGMVLTEVQR"
BASE COUNT         1369 a         1132 c         1170 g         1224 t
ORIGIN      
        1 cgtgcttttg cttgtcctcg cgttttcgaa tgtaaacgcc gcggcaaaag atgaatggct
       61 cgcggtgcgc tcaaaaaatt tttatctgat cggcaatgcc agcgacaagg aaattcgcaa
      121 agtcgccacg cagctcgaac aatttcgcga ggtttttcgc caaatcctca acaaggtaaa
      181 ttttaactcg ccgattccga cgaccgttat cgtttttaag gacgacaagg cttacaaccc
      241 gtttaagccg gtcagggcga ccggaaagat aaacaaaaac atcgccggtt attttatgcc
      301 cggcagcgac gtaaactata ttacgctcgc catccagagc gatatgagcg aggtttacgg
      361 cacgattttt cacgagtaca cgcattttct ggtaaacaac aatatcggaa aatccaacgt
      421 gccgccgtgg tttaacgaag gcttggcgga atattatcaa accttcaaaa tcgaagacga
      481 ccagaaagtg acgctcggca gcctgcaaaa cggacatctc tattcgcttc agcaaaataa
      541 gctgattccg ttcgatacgt ttttcaatat cgacaattac tcgcttcacc agcagggcga
      601 ccacggcacc aatatgtttt acgcgcagtc gtgggcgctg atgcattatc tgatacacgg
      661 cgagggcggc gcgcgcagca agcagatgaa taaatttctc gacctcgtga tgaacggcag
      721 gcaggcgcgc gaagcgtttt cggaagcgtt tcagaccgat tacgcgacga tggaaaagga
      781 attgaaaaaa tacgtcgaga aaagcagtta caaagtcacc ggaatcgttt tcaaaaacaa
      841 attgacgttt gataccgaga tgcgaaccgc gccgctgacg cagcccgagg ggcaggctta
      901 tctcggcgat ttgctttatc acatgaatcg cctgggcgac gccgaaacgc ttttgcagca
      961 ggttttaacg ctcaatccgg attcgagcct ggcgcagacg tcgctcgggc tggtaaaaat
     1021 gcggcagaaa aattttgacg aagcaaaaaa atatctggaa aaagccgtcc agagcgattc
     1081 gagtaattat ctggcgcact acagctacgc ctacgttctc agccgcgaag cgatgaacgg
     1141 cggcaatttt gcaagcgggc tggagccgga aagcgccgat aaaatgcggc aggcattgaa
     1201 gaaagcgatt gcgctgaatg ccggatttgc cgaatcctac gaccttttcg cctttatcag
     1261 cgtcgtccgc aacgaaaatc tcgacgaagc cgtgctgtat atgaaaaaag cgctgcaaat
     1321 cgctcccggc aaccagcaat atatgattcg cgccgccgag cttttgatgc ggaaaaaaga
     1381 atttgccgaa gcgaaagtga ttgctcagaa agttgtggaa actgccgacg aaccgcagtt
     1441 aaaaagctat gcccagagcg ttttatccag catcaactct tacgagagag ggctcgaaga
     1501 gatgaaaaaa tataacgagc gcggcggcgg cattattagc agtactacga caattgctga
     1561 cgacaaaccg atgagcgagg aagaaatcgc caaaacgcgc gccgaaatgc agttgaaatc
     1621 gctcaataat attttgcgaa aacccaacgc agacgaaaaa agagttctcg gctatctgac
     1681 gaaaatcgac tgcacggcaa aaggaatgac atacaccgta aaagttgacg ggcaattgat
     1741 gaaattcacc agcacgtcgt ttgatacggt ggaaatgata acggtcaacg tcaatctcga
     1801 agacggtcag atcaactgcg gcgctctgaa aaaggaaaca ttcgccctcg tcacctatcg
     1861 cgcgggcaaa gacgccaaaa cagcgggcga aatcgtcgcc ttcgaattcg tccccgaagc
     1921 attcaaactg ttgaattaag ttatttaatt ccggtttttc cgaattgaat ttcgctcgaa
     1981 gggcgcgtgt cggcatgcag cacgcgctct atatatttta aagccttttc cttttcttcc
     2041 gcctcggtcg ccgccacgca gtcgcgcggc acaataattt taaaatcccg cagataagcg
     2101 tcgttcgccg taaacaaaac gcaaatatca gccgtcagac cggtcaaaat caaagttttc
     2161 gcgtttagat attcgagcag caaatccagc gtcgtcgaat aaaaacccga atgtttcggc
     2221 ttaaggacaa aataatcttt cgctttcggt ttcaattgtt tggcaaccgc gtgacctttc
     2281 gacgttttcg ccgaagcgtg tttgagcagt tttttgaaat ccgactgcca tttcccgaaa
     2341 ttgtcgttga tataaattac gggaattttg gctttatcgg ctttgtcccg caaggcagcg
     2401 atatttttta caatcggctt ggtattttcc agtaatttat cgccgtcagg aaaatcaaaa
     2461 tcagagatga catcgataat cagcaagaca atcggagaat tctccggcgc gtttccgtgc
     2521 aaatcattat tcttcgtcgg catatcagta tttcaacaaa agacaacgaa aaaaggcaag
     2581 ccggatttat tgttcccgaa atgaactgcg ccaaaaaatg tggagttata cagtcttttg
     2641 ttataaaaac ggcaggcgag cgaagcttga aacgtcgatg aattttaatc ggctaaataa
     2701 ttttggaagt ggtgattcgg gcggattttg taacgggtat agagcgcatt tgcaggaaaa
     2761 tttgcaaatg cgccgaagcg gtaatgaata gctttttgaa tacttgtaat ttttaaaacg
     2821 ccttctaaga ttaccttttg gaaggcattt ttttttctct gcgtttgttt tctttcataa
     2881 cgcttcgatt tcgtcaattc caataacttt tgccagcaaa ataatcatat cccgcgcgct
     2941 taccgctctg cccgttttcg atgcgtgtga gtccggtgcg gttaaattag ctttgcctgc
     3001 gacgaatttt tgattcagac cgcgtgtttc tctttccttt tgcagtcaga tgctgaattc
     3061 ccggcaaaac ttcgctcggc ttaggcttcg aaatgacaaa gaatgttaat gaaatgtttg
     3121 ttgacattat aatcaattta tcattaacat tgaatgtcaa atcaaataat gcctgaggaa
     3181 atatgaagaa aataattgca gtaattcaag aaaaatttag tgcaatcatt acggttaagc
     3241 tttacatgtg atgcaaaata ttgtaattat acttaacata aaatatcaat attacgcgat
     3301 tcggaattcg ggttaaattg caatcagatt attcaagaaa atggcacatc gaacgtccgg
     3361 aagtaatttg agaaagtttt ttcccgcaga cgccattcag cggcaagagc aattgtcggg
     3421 aaaagcccat aaacttatga acaaaataaa agccaaacat ttagccaccg gcgatagagt
     3481 cagattttca cgcgacggct ggcaaactgt cggacgcata acgaactgca attccatcag
     3541 cgtttctttt atgttcggcg acggcattat ccgaaagctc gaagctaatc aggagtacgt
     3601 cgtcgaacag gcggaacagc cgcccgagcc gcaacccgga caaattatgc gcctgcaaaa
     3661 agaatcgaat cgggtttcag tttgcgcgtc gcattctttg tttgatttga gcgacatcag
     3721 cgaagcgttt cgagcgataa aacaaaaaga cggatattca aacagctcgg cggcttatca
     3781 cgagctggaa agaggcgcga ttttgcacac gcacgatgcc gtttactgga tgccgctcaa
     3841 atggtcgggc gaggcgacca aagaaaagta accaaaaacg tttgtaaaaa agacgcatat
     3901 tattgtctgc gagacggcga gaaaatttct caggcgtgcg gcgagtgaga cggttattcc
     3961 tcaaaaactc tgacataaat attattcaag ctttaatttg ccgccgccac gcggcagcag
     4021 tcgttttgcg caagcaaatt tattgataaa aaccggcgag ttgtctataa ttactccgtt
     4081 tccggttcaa tctgttaagg cttttcgaat aaagggttat aagttcagga atcgccacac
     4141 ttcctgtaaa gagaggaata ttgctcttgc atctgtcatt aaatcttccg cgccgcaaaa
     4201 aattaatcac ggttttcgca gttttagcgt ttttgctttt actttttatc ccgatgctta
     4261 cgacgcaggc ggcggcgggc gcgctcgacc cggcttttgc cggcggcggg atgacgcctt
     4321 cgctcggcgc aaattttttc agcgcttcga gttttgccgt ccagcccgac ggcaaaatta
     4381 tcgtcggcgg agtgtattcg ctttcgaccg aagagccttt tgcaaatgcg caatttgcgc
     4441 tcgttcgcta tctgccgacg ggcattatcg attcgagttt cggaacgaac ggtcgcgtcg
     4501 tgacgacatt tacagaatat ccggcgacgg caatgtctct gaatgatatc gccgtcctgc
     4561 cggacggaaa aatcgtcgcg gtcggttcgg gcaggcttgc cgccgagacg cctcatttaa
     4621 cgcggctgag tcttttcgtc gttcgataca acgcggacgg ctcgctcgat acgccgttca
     4681 gcaacttcgg taaatttgtt tcacacgcgg gcggtattca gacgacggcg caggcggtgg
     4741 cagtgcagcc ggacgggaaa ttcgtcgtcg ccggatacgc gggcggcgct atcaattcgc
     4801 tcgtgccgta taaatttatt ctgttcagat tttccggcaa tggacccgac ccgactttcg
     4861 gcaataacgg aatggttctg acggaagttc agaga
//
LOCUS       DASWNE010000009         2922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_212226, whole genome shotgun sequence.
ACCESSION   DASWNE010000009 DASWNE010000000
VERSION     DASWNE010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212226"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="VGB68_00260"
     CDS             <1..895
                     /locus_tag="VGB68_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047686.1"
                     /translation="PTRLKCFYCGAELPVSAEQSEFIKPQPRKMETWEKGFNLIILPG
                     ARACDDASLGEIAGMTRLEKEDLRKLIDAQKPLPLTRADSEKEAKIIARRLSENNLES
                     LILSDDEDLKIETVTRRLRGIEFGDDKLVLILFNSDEIAEIRREDLSLIVSGALFERR
                     IETTEQRKKRENKILETTETSADELLLDIYPRADAVGYRIISGGFDFSCLETEKGILA
                     RENMRKLEEKLRAFAAQAKFVGDYLQIRDVLGKVWEVEEKRDSRGLQRKGFGRLDFGN
                     VTTINNLSQFTKYSRLQRHLL"
     gene            892..1929
                     /gene="rlmB"
                     /locus_tag="VGB68_00265"
     CDS             892..1929
                     /gene="rlmB"
                     /locus_tag="VGB68_00265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEY0047687.1"
                     /translation="MKAKGKRQKAKSNSRLEPGAAGGFQGGNQRVLKVADSAKEKNRP
                     QLNAEKHGFEKQARLKSQTANPKFQTEDRRPKTEDRFSTDNEPRAANIIFGVLPVLES
                     LRAGARRIEKIFVAEGAHEKRLQEIFDLVRQAGIPFRKIPRQNFSKFLEPGVNHQGVA
                     AFVASAEYADADGLIEEITTKEISLSVILDGVEDPRNLGAILRTIECSGTDGVFIPER
                     RAVGLTETVAKSSAGATEYVKVAKVGNVNRLIEQLKENNIWVIGTSGEAKIDYTEWDW
                     TRNCALVLGSEGKGLHRLTAEKCDVLVKIPLLGRIESLNVSVAAGVILYEAVRQRNLQ
                     RNAAEKETTDD"
     gene            1952..2545
                     /locus_tag="VGB68_00270"
     CDS             1952..2545
                     /locus_tag="VGB68_00270"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY0047688.1"
                     /translation="MFCPKCGTQNPENGKFCRACGTGLDNAAAVISGGNLPAQFTDAG
                     VAHIHHEARRRQDPNELYGDGVRNIILGIGFLIVSMALLLTNVAGGKAWWWAMLFPAF
                     GMLSSGISCYLKHRRLEKRQSASLNQNQINQTANLFNQPPPNSALPPTQTDYVRPQRK
                     SIYDTGELYEKPSSVTEGTTRHLEINNEGETMTLPKK"
     gene            2566..2880
                     /locus_tag="VGB68_00275"
     CDS             2566..2880
                     /locus_tag="VGB68_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047689.1"
                     /translation="MIDIKDIIEKDVLDMKDVTEIAKQFIFELNGEQEQFQVEEIMLS
                     EDRKNWLITVSYYRKIKSQNELQKTLGLEGSRVYKRIVIEREKYHVIGMFNWTYDRRE
                     AA"
BASE COUNT          863 a          661 c          795 g          603 t
ORIGIN      
        1 gccgacgcgc ctgaaatgtt tttactgcgg cgcggagctt ccggtttcgg cggagcagtc
       61 ggaatttatc aaaccgcagc cgcgaaaaat ggaaacctgg gaaaaaggct ttaacctgat
      121 tattttgccg ggcgctcgcg cgtgtgatga cgccagcctc ggcgaaattg cgggaatgac
      181 gcggctggaa aaggaagatt taagaaaatt aatcgatgcg caaaaacctc tgcccttgac
      241 gcgcgccgat tcggaaaaag aggcaaaaat cattgcgcga agattaagcg aaaacaatct
      301 tgaaagcctg attttaagcg atgatgaaga tttgaaaatc gagacggtga cgcgccgcct
      361 gcgcggaatc gagttcggcg acgacaaact cgttctgata cttttcaact cggacgaaat
      421 cgcggaaatc cggcgcgaag atttgagctt aatcgtcagc ggcgcgctgt ttgaacgccg
      481 catcgaaacg accgagcagc gcaaaaaacg cgaaaataaa attctggaaa caaccgaaac
      541 cagcgccgac gagcttttgc tcgacatcta cccgcgagcc gacgcggtcg gttatcgaat
      601 tatttcgggc ggcttcgatt tttcctgtct ggaaacggaa aagggaattc tggcgcgcga
      661 aaatatgcgg aaactcgaag aaaaactccg cgcgtttgcg gcgcaggcaa aattcgtcgg
      721 cgattatctg caaatcagag acgttctcgg aaaagtctgg gaagtcgagg aaaaacgcga
      781 ttcgcgcggg ctgcaaagaa aaggcttcgg gcggctcgat ttcggaaacg tgacgacgat
      841 taacaatttg agccaattta caaaatactc gcgtctgcag cgacatcttt tatgaaggca
      901 aaaggcaaaa ggcaaaaggc aaaatcaaat agtcgtctgg aaccgggcgc ggcgggcgga
      961 tttcaaggcg gaaatcagcg cgtccttaag gtcgccgatt ccgcgaaaga aaaaaatcgc
     1021 ccgcagttaa atgcggaaaa acacggattt gagaagcaag cccgactcaa atcccaaaca
     1081 gcaaatccca aattccaaac cgaagaccga agaccgaaaa ccgaagaccg tttttcgacc
     1141 gacaacgagc cgcgagcggc aaacataatt ttcggcgttt tgccggttct ggaatctctg
     1201 cgcgcgggcg cgcggcgaat cgagaaaatt tttgtcgccg aaggcgcgca cgaaaaacgc
     1261 ttgcaggaaa tctttgattt ggtcaggcaa gccggaattc ctttccggaa aattccgcgg
     1321 cagaattttt caaagtttct ggagccgggc gttaatcatc agggcgtcgc cgcttttgtc
     1381 gcttcggctg aatatgccga tgcggacgga ttgattgaag aaatcacgac caaagaaatt
     1441 tcgctgagcg tgattctgga cggcgtcgaa gacccgcgaa atttgggcgc gattttgcgg
     1501 acgatcgaat gtagcggcac ggacggcgtt tttattcccg aacgccgcgc cgtcgggctg
     1561 acggaaaccg tcgccaaatc ttcggcgggc gcgaccgaat acgtgaaagt ggcgaaagtc
     1621 ggcaatgtca atcgcttgat cgagcagttg aaagagaata acatctgggt tatcggcacg
     1681 agcggagaag cgaaaatcga ttacaccgaa tgggattgga cgcgaaattg cgcgctcgtt
     1741 ctgggcagcg aaggcaaagg cttgcatcgc ctgacggcgg aaaaatgcga cgtgctggtc
     1801 aaaattccgc tgctcgggcg gatagaatca ttgaacgttt cggtcgccgc gggcgtaatt
     1861 ctttacgaag cggtcagaca aagaaattta cagcgaaacg ccgccgaaaa agaaacaacg
     1921 gacgattgac caaagacaaa ggacgaagat tatgttttgc ccgaaatgtg gaacacaaaa
     1981 cccggaaaac ggaaaattct gccgcgcctg cggaacgggt ttggacaacg ccgcggcggt
     2041 catcagcggc ggcaatctgc ccgcccagtt taccgatgcg ggcgtcgcgc atattcatca
     2101 cgaagcccgg cggcgacaag acccgaacga gttatacgga gacggggtga gaaacattat
     2161 tttaggcatc ggttttctga tcgtctcgat ggcgctgctg ctgacaaacg tcgcgggcgg
     2221 caaagcctgg tggtgggcga tgctttttcc ggctttcggt atgctgtcaa gcggcatttc
     2281 ctgttacctg aaacaccggc gtctggagaa aaggcaaagc gcgtctttaa atcaaaatca
     2341 aattaaccaa acggcgaatc tgttcaacca gccgccgccg aattccgcgc tgccgccgac
     2401 gcagaccgac tatgtcagac cgcagcggaa atcaatttac gatacgggcg agctttacga
     2461 aaaaccgtcg agcgtcaccg aaggcacgac gcggcatctg gaaattaata atgaaggcga
     2521 aacgatgact ttgccgaaga agtaaaattt tgtataaggt gaattatgat tgatataaaa
     2581 gacatcattg aaaaagacgt tcttgatatg aaagatgtga ctgaaatagc caaacaattt
     2641 atctttgagt tgaatggcga acaagagcaa tttcaagttg aggaaattat gctttccgaa
     2701 gataggaaaa attggctcat taccgtaagt tattacagaa aaattaaatc acaaaatgaa
     2761 cttcaaaaaa cattaggttt agaaggcagc agagtttata aaagaattgt catcgagcgt
     2821 gaaaaatatc atgttattgg tatgtttaat tggacttatg atagacgcga agcggcatga
     2881 atgattattt gctgaaactc atccaaaagt ataggcagaa gg
//
LOCUS       DASWNE010000010         7874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_353783, whole genome shotgun sequence.
ACCESSION   DASWNE010000010 DASWNE010000000
VERSION     DASWNE010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7874
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353783"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VGB68_00280"
     CDS             <1..633
                     /locus_tag="VGB68_00280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0047690.1"
                     /translation="PKDESSVVRFVSSDKIEVIQDFTTSVNSLNNALDNLFVDGGRTA
                     VVDAVYLAAKKVGQYQNSRKKEDARLRALILISDGDDRSSSYREEELFALLREQQVQI
                     YAIGFVNDLTNAPDPSSQLNRQEKAKTFLTRLAQETGGKVYFPNSIEELPQIALDISR
                     ELRTQYLISYSPTSENGGGSFRKIRVLVSDGANGEKRTAVTRAGRNSLSK"
     gene            complement(776..2968)
                     /locus_tag="VGB68_00285"
     CDS             complement(776..2968)
                     /locus_tag="VGB68_00285"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylase"
                     /protein_id="HEY0047691.1"
                     /translation="MISANLRYIRYKYLLILTFLLLSIPVSGSDGENLSRWERQARNV
                     SIVRDDWGIAHITGKTDADAVFGMIYAQAEDDFNRVETNYLNALGRLAEAEGESAVWR
                     DLRMKIFIQPEELKKKFAESPLWLQKLMTAWADGLNFYLAGHPEVKPRVITRFEPWMA
                     LSFTEGSIGGDIETISLARLQSFYDSPPAAAKFDEPDFLAEPRGSNGIAIAPKNTLGG
                     NALLLINPHTSFFFRSELQVTSGEGLNAYGAATWGQIFIYQGFNERLGWMHTSSAVDA
                     IDEWRETVFKKGDKYFYRFGGEERPLLTSVIRVPYKTARGMAERSFTIYRTHHGPVVR
                     RDEASGNWVSVGLMNDPVKALTQSFTRTKARNYREFRQTMQLHANSSNNTIYADADGN
                     IAYFHVNFIPRRNPRFDWTKPVDGSDPETDWKGLLSFEESPHLFNPASGWLYNSNNSP
                     WSAAGSSSLKQADFPKYVDNGIENARGLHAIKLLENRRDFTLEKLQEAAYDSYLPWFE
                     KTVPALVAAWDKLPPNDPLKTKLREQISILRQWNLRWSTDSVAASLAVFWGAEILRAL
                     GPSPGVPSQDNVAAKATPAQLLESLTRASNRLAADFGNWRTPWGEINRFQRLTGDIVH
                     PFDDAAPSVPVGFTSGNWGSLASFGAAPTGSTRKWYGTSGNSFVAVVEFGKKIRAKAV
                     TAGGESGNPKSPHFNDQALRYAKGDLREVYFYPAQLKGHTERKYVPGN"
     gene            complement(3173..6076)
                     /locus_tag="VGB68_00290"
     CDS             complement(3173..6076)
                     /locus_tag="VGB68_00290"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY0047692.1"
                     /translation="MNCYSIILKRKFLLSATLGVLILFSAPAFAQQTGALGGQVTDEL
                     GGAVEGSEIALTDRNGKTKKTFTDKTGSYSFKNLTPGTYSLQVASKDFAFFEIKEVLV
                     TAARPQSLDIRLKVREITESVAIDDGSTTKAQPSSNFGDVTLREKEVADLPDDPEALA
                     AALKAMAPVGPGEPQILVDGFTAENPPPKQTIREVRISQNNFTAEDDRPGGSRIQIFT
                     KSGLDKLQGNAFFNWSDESLNARNPFAEERAPYLYRQYGFSLSGTLIPKKGSFFLNFQ
                     KLDENQNGIVSAQTLDGSLNPLPLNLSFIIPRRNYYFNPRLDFQLSKNHSLTLRYGYS
                     RSAIKNLGVGELYLPERGYDYRNSQHIFQATETAIINPQTVNEFRLQYVRSALDLSDT
                     NSTPGINVQGAFLGGGAGIGLSSNKSDRFELQNYVTTNTKNHLMRFGVRLRALRLKDT
                     SPGNFNGSFTFAGGSAPVLDGNYQIVLDSSGQPVLTQITSLERYRRTLLLQGLGFSAA
                     AVRLRGGGATQFSIALGNPEVEVKQTDFGAFFQDEWRLKPNLNIYLGLRYENQTNLDD
                     NLNFAPRAAFAWVPKIGKENTTIRGGLGLYYERFSEQYTLQAQQFGGSQIQRIISSDP
                     NLVDFFPHLPSPAAISSFLDKQTTTKISDDIKAARAFVSLINFEHKFSSKHSVYAGLS
                     TYRTWHSVRQRNINAPLPGTYILGQPESGIHPFGDIGEIFLVESSNEYLQNQFYAGFR
                     SQINSAFFVSANYLLSDTKDAGSAGAFPADSYNLQSEWGRASAFSIRHRLYLSGRIEI
                     PKMKMTFTPQLIVFSQRPFNITTGLDANGDQVFAERPSFAAATAGGINIYNTPFGVFN
                     ILPPAGEQIIPRNIGRGAPFFSVNAGIGKTFGFGSSGKTNADSKNSADKPYKITFSVQ
                     IQNLFNNVNYGTFIGNLSSPLFGKATRTVGAYGFEDGSPAYNRRIEAQIRFSF"
     gene            complement(6115..7206)
                     /locus_tag="VGB68_00295"
     CDS             complement(6115..7206)
                     /locus_tag="VGB68_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047693.1"
                     /translation="MKKYLIVISTIILVFGGCRALQAQSTSSSSAGGNNPVNVFGKIS
                     NINLDGNQITILTTDGKNIVVAFDKAVEFKQVPAGETTLRNATDITPAQINAGDNALV
                     RGNRTSEDNILARQIIVVTQADIIKKEAQRREEWKLRGIAGIVRKIDLGARKIFVQTK
                     GANSQLIEVVPTDKTIFQRYETGAGSLNDFKPGNLEKIREGDQIRAIGDKSADGLQFV
                     PEEVFSGSFRILTGKVLSIIPETGELRLSDLQNGKTVTAVVNSASNLRRLTEDSVKSE
                     AASAAKGSAPETAAGGSINLTSLLEKSPKINIADIKIGETIVIASLSDAGAERIIGIT
                     VVSGVEILNIRAGNKSGSNANRKFNLDVF"
     gene            complement(7251..>7874)
                     /locus_tag="VGB68_00300"
     CDS             complement(7251..>7874)
                     /locus_tag="VGB68_00300"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0047694.1"
                     /translation="EGSILQELDFRSGEGEKILVLGPNGSGKSTFAHLVSGLLSPSSG
                     LLTTFPLAKVSAVIHPFDFIPGSVTDNLSFAASDAQKRKFARLAAEFGLDKHLEKDPV
                     ELSAGQRKKLEITMGLVKDSDIYIFDEPLAGVDVGSKETVVNAIFEHTKDKILIVIMH
                     GDERFHDLFDRKIDLEAAQAESLRLSKKGKISPVPEPEGEAAEIVCV"
BASE COUNT         1834 a         2034 c         2029 g         1977 t
ORIGIN      
        1 ccgaaagacg aatcgagcgt tgttcgtttc gtcagttccg acaaaatcga agtcattcag
       61 gatttcacca ccagcgtcaa ttcgctgaac aatgcgctgg ataatttgtt tgtggacggc
      121 gggcggacgg cggtcgtcga cgccgtttat ctggcggcga aaaaggtcgg gcagtatcag
      181 aattcccgga aaaaggaaga cgcgaggctt cgcgcactga ttctgatttc cgacggcgac
      241 gaccgctcca gctcttatcg ggaagaagaa cttttcgcgc tgctgcgcga gcagcaggtt
      301 cagatttacg ccatcggatt tgtcaatgat ttgaccaatg cgcccgaccc gtcgagccag
      361 ctcaatcgcc aggaaaaagc gaaaactttt ttaacccggc tggcgcagga aacgggcgga
      421 aaggtatatt ttccgaattc gattgaagag cttccgcaga tagctctgga catttcgcgc
      481 gagctgcgca cgcagtattt gatttcctac agtccgacca gcgaaaacgg cggcggaagt
      541 tttcggaaaa tcagagtgct ggtatccgat ggggcgaacg gagaaaaaag aaccgccgtc
      601 acgagggcgg gaagaaattc cctgtcgaag taaaagccct gaaacggagt tccaagccgg
      661 ttacgggcaa atcgacgcgc ggcgcgaatt tacccggttc gggcgcaaat ccggtttgct
      721 gagcgatttg ccgacgccgg ataccgaatt cgccggttaa aaatcaaccc cgacctcaat
      781 tgcccggaac gtatttcctc tcggtatgac cttttaactg ggctggataa aaatacacct
      841 cgcgcaagtc gcctttggcg tagcgcagcg cctgatcgtt aaagtgagga gatttcgggt
      901 tgccgctttc accgccggcg gtcaccgcct tggcacgaat ctttttgccg aactcgacca
      961 ctgcaacaaa gctgttgccg ctcgtgccgt accatttcct ggtgctgccc gtcggcgctg
     1021 ccccgaacga tgccagagag ccccagtttc ccgaagtaaa tccgacggga acgctcggcg
     1081 cggcgtcgtc gaaaggatgc acgatatctc ccgtcaggcg ttggaagcga ttaatttcgc
     1141 cccagggcgt tcgccaattt ccgaaatccg ccgcgagcct gttcgatgcg cgcgtcagcg
     1201 attcgagcag ttgagcggga gttgcttttg ccgcgacgtt atcctgtgac ggcacgcccg
     1261 gactcggacc cagagcgcga agaatctccg cgccccagaa gaccgcgagc gaggctgcaa
     1321 ctgaatccgt tgaccagcgc aggttccact gccgcagaat gctaatctgc tccctcaatt
     1381 ttgtctttaa cggatcattt ggaggcaatt tgtcccacgc cgcgacgagc gccggaacgg
     1441 tcttttcaaa ccacggcaga taactgtcat aagccgcttc ctgcaatttt tcgagcgtga
     1501 aatccctgcg gttttccagc agcttaatcg cgtgcagccc gcgcgcgttc tcaataccat
     1561 tatcgacgta tttcgggaaa tccgcctgtt tcaggctgct cgaaccggcg gctgaccacg
     1621 gagagttatt gctgttgtaa agccatccgc tcgccggatt aaacagatgc ggcgattctt
     1681 cgaacgagag caaacctttc cagtcggttt ccgggtcgct cccgtcgacg ggtttcgtcc
     1741 agtcgaatcg aggattgcgc cgcgggataa aattaacgtg aaaataggcg atattgccgt
     1801 cggcatcggc ataaatcgtg ttgttcgaag aattcgcgtg cagctgcatc gtctgccgga
     1861 attccctgta atttctcgcc ttggttctgg taaaagattg ggtaagcgcc ttgaccgggt
     1921 cattcatcag cccgacgctg acccaattcc cgcttgcctc gtcacgcctg acgaccggtc
     1981 cgtgatgcgt gcggtaaatc gtaaacgaac gctccgccat tccccgcgcg gttttatacg
     2041 gcactctgat aaccgatgtc aggagcggtc tctcctctcc gccgaacctg tagaaatact
     2101 tatcgccttt tttaaaaacg gtttcgcgcc actcgtctat cgcatcgacc gcgctcgaag
     2161 tgtgcatcca gcccagacgc tcgttaaagc cctgatagat gaagatttgc ccccaggtcg
     2221 ccgccccgta agcattaagc ccttcgccgc tggtcacctg caattccgag cgaaagaaaa
     2281 atgaagtatg cggattgatg agcagcaggg cgtttccgcc gagcgtgttc ttcggagcaa
     2341 tcgctattcc gtttgagcct ctcggctcgg cgagaaaatc cggctcatca aatttcgccg
     2401 cagccggcgg cgagtcgtaa aaggactgaa ggcgggcgag gctgattgtt tcgatatcgc
     2461 cgccgatgct gccctctgta aacgatagag ccatccaggg ctcgaaccgg gtgatgacgc
     2521 gcggctttac ttcaggatgc ccggcaagat aaaagttcag accgtccgcc caggcagtca
     2581 tcagcttttg cagccacaaa ggactctccg caaatttttt ctttaattcc tcgggctgga
     2641 taaagatttt cattcgcaaa tcgcgccaga cggcggattc gccttcggct tcggcgagtc
     2701 gccccaaggc gttgagataa ttggtttcga cgcggttgaa atcgtcttcc gcctgagcgt
     2761 aaatcatacc gaatacggcg tcagcgtccg ttttgccggt gatgtgggcg atgccccaat
     2821 catcgcggac gatggaaaca ttcctcgcct gccgttccca gcgcgataga ttttcgccgt
     2881 ccgacccgga gaccggtatc gacagcagaa ggaaggtcag aataagtaaa tacttgtaac
     2941 gaatgtaacg gagattggct gaaatcatat tgtctttgat ggaaataatt tattgttgcc
     3001 gattgtttag acgagcgcaa acaaaagagt gttataaagc gacaggaagc gcaatttatg
     3061 aataaatcga caatgccgtt tttcttaaat tcggagcctg cacgaattga tataaaaggt
     3121 taagcctctt atatacaata tttgacgctg ataccgaaac ttaattcgct atttagaaac
     3181 tgaaacggat ttgagcttct attcttcgat tataagccgg gcttccgtct tcaaatccat
     3241 atgcgccgac ggttcgggtc gcttttccga acaggggcga gctcagatta ccgataaagg
     3301 tgccgtaatt tacgttattg aaaaggtttt gaatctgcac ggagaatgta attttgtaag
     3361 gcttgtctgc ggaattcttt gaatcggcat ttgttttgcc ggacgagccg aagccgaagg
     3421 ttttgccgat tcccgcgttg actgagaaaa atggcgcgcc gcgcccgatg tttcgcggaa
     3481 ttatctgctc gcccgccggc ggaagtatat tgaaaacgcc aaacggagtg ttgtaaatat
     3541 tgattccgcc ggcggttgcc gcggcaaacg aagggcgctc cgcaaatacc tggtcgccgt
     3601 tggcatcgag cccggtcgtg atgttgaaag ggcgttgaga gaaaacaatg agctgcggag
     3661 taaacgtcat tttcattttc ggaatttcta ttctgccgct caaatacaag cggtggcgaa
     3721 tactgaaggc ggaagcgcgc ccccattcgg attggagatt ataagaatca gccggaaacg
     3781 cgcccgccga accggcgtct ttggtatccg ataacaaata atttgccgaa acgaaaaacg
     3841 ctgaatttat ttgacttcta aacccggcat aaaactggtt ttgaagatat tcgttactcg
     3901 attcaaccag aaaaatctct ccgatgtcgc cgaacgggtg aattccgctt tcgggctgac
     3961 cgagaatata tgttccgggc agcggagcgt ttatattgcg ttgtctaacg ctgtgccagg
     4021 tgcgataggt ggaaagtccg gcgtaaacgg aatgtttgga agaaaattta tgctcgaaat
     4081 taatgagcga gacaaatgcg cgcgcggctt ttatatcgtc cgagattttc gtcgtcgttt
     4141 gtttgtcgag aaaagacgat atagcggcgg gcgagggaag atgcggaaaa aaatcgacca
     4201 gattcgggtc ggagctgatt attcgttgta tctgactgcc gccgaattgc tgcgcctgaa
     4261 gcgtatattg ttcgctgaat cgctcgtaat ataaacccaa accgccgcga atcgtcgtat
     4321 tttcctttcc gatcttcgga acccaggcaa aagccgcgcg cggcgcgaaa ttgagattat
     4381 cgtcgagatt cgtttgattt tcgtaacgca atcccagata aatgttcaga ttcggcttca
     4441 gtcgccattc atcctgaaaa aacgcgccga aatccgtctg tttgacttcg acctcgggat
     4501 ttcccagtgc aatcgaaaac tgggtagcgc cgccgccccg caatctgacg gcggcggcgc
     4561 taaatcccaa tccttgtaat aataatgtgc ggcggtagcg ttccaggctc gtaatttgag
     4621 taagcaccgg ctgacccgaa gagtcgagaa caatttgata gttgccgtcc agaaccggcg
     4681 cgctgccgcc cgcgaaggta aaactcccgt taaaattgcc cggagaggta tccttcagtc
     4741 gcaaggctct gagcctgacg ccgaatctca ttaaatgatt ttttgtgttt gtcgtgacgt
     4801 aattttgcag ctcgaatcgg tctgatttgt ttgacgataa gccgatgccc gcgccgccgc
     4861 cgagaaatgc gccctgcaca ttaatgccgg gcgtcgaatt cgtgtcgctt aaatcgagcg
     4921 cgctgcggac atactgaagg cgaaattcgt tgaccgtctg cgggtttatg attgccgttt
     4981 cggtagcttg aaaaatatgt tgcgaattcc ggtaatcgta gcctctttcg ggcaggtaca
     5041 gctcgccgac gccgagattt tttatggcgg atcggctata accgtaccgc agagtcagcg
     5101 agtgattttt gctcaattga aaatcgagac gcgggttaaa gtaataattg cgccgcggaa
     5161 tgataaatga aagattgagc ggcagagggt ttagagagcc gtcgagagtt tgcgcgctga
     5221 cgattccgtt ttgattctcg tccagcttct ggaagtttag aaagaacgag ccttttttgg
     5281 gaatcaacgt cccgctcagt gaaaaaccat actgccggta aagataaggc gcgcgctctt
     5341 cggcaaacgg attgcgcgca ttcagacttt catcgctcca gttaaaaaag gcattgccct
     5401 gaagtttatc cagaccggat tttgtaaaaa tttgtattcg cgagcctccc ggacgatcgt
     5461 cctcggcggt aaagttgttt tgactgatgc gcacctcgcg aatcgtctgt ttcggcggcg
     5521 gattttccgc cgtgaagccg tcgaccaata tctgcggctc gcccggtccc acgggagcca
     5581 tagcctttaa ggctgccgcc agcgcttcgg ggtcgtccgg caggtcggcg acctcttttt
     5641 cacgaagagt aacatcgccg aagttgctgc tcggctgagc ctttgtcgtc gaaccgtcat
     5701 ctatcgctac cgattcggta atttccctga cctttaaacg gatatcgagt gattgcgggc
     5761 gagcggcagt tactaatact tctttaattt caaaaaaagc gaaatcttta ctggcaacct
     5821 gtagggaata agtgccgggc gtcagatttt taaaggaata actgcctgtt ttgtccgtaa
     5881 aagtcttttt cgttttaccg ttccggtcgg ttagcgcaat ttcggagccc tcgaccgcgc
     5941 cgcccagttc gtccgtaacc tgcccgccga gcgcgccggt ttgctgagcg aaggctggag
     6001 ctgaaaaaag aatcagaacg ccgagcgtcg ccgataacag gaatttcctt tttaaaatga
     6061 tagagtaaca attcatagca ggcgttcctt taaacctccc ggacagccga aactttaaaa
     6121 tacgtcgagg ttgaatttcc tgtttgcgtt tgagccggat ttatttccgg cgcggatgtt
     6181 taaaatctca actccggaaa ctaccgttat cccgataatg cgctcggctc cggcgtccga
     6241 gaggctggca atcacgattg tttcgccgat tttaatgtct gcaatgttta ttttcggtga
     6301 tttctcaagc agcgaagtca aattaatact tcctcctgcc gccgtctccg gcgcgctgcc
     6361 tttggcggct gaagccgctt cgcttttaac cgaatcttcg gtcagccgtc gcaggttcga
     6421 agcggaattg acgaccgctg tgacggtttt gccgttctgc aaatccgaca gccgaagctc
     6481 gcccgtttcc ggaataatcg acagaacctt gcccgttaat attcgaaacg aacccgaaaa
     6541 aacttcttcg ggaacaaatt gcagcccgtc ggcgctttta tcgccgatag cccgaatctg
     6601 gtcgccttct ctaatctttt ccagattgcc gggtttaaag tcattcaaac ttccggctcc
     6661 ggtttcgtag cgctgaaata tcgtcttatc ggtcgggaca acctcgatta attgagaatt
     6721 tgcgcctttc gtctgaacga aaatttttcg cgctccgagg tcaatctttc ggacaatgcc
     6781 ggcgatgccg cgcagtttcc attcctcgcg tctctgcgct tcttttttaa taatatctgc
     6841 ctgcgtgacg acgattattt gccgggcaag gatattatct tcactcgtcc gatttcctct
     6901 gaccagggcg ttgtctccgg cgtttatttg cgcgggcgta atatcggtcg cattgcgaag
     6961 cgtcgtttct cccgccggaa cctgcttaaa ttcgacggct ttatcaaatg cgacgacgat
     7021 atttttcccg tccgtcgtga ggattgtaat ttgattgccg tcgagattta tattcgagat
     7081 ttttccgaaa acattaaccg gattattgcc gcccgcagaa gaagaagatg tcgattgcgc
     7141 ctgaagggct cggcagccgc cgaatactaa tattatcgtc gatattacaa tcaggtattt
     7201 cttcataatc cgtttttgca ggctgccgat ctcactcgcg gacggacttt ttagacacag
     7261 actatttccg ccgcctcgcc ttccggctcc gggacgggag aaattttacc tttctttgaa
     7321 agcctgagcg attcggcttg agccgcctct aaatctattt ttcggtcgaa caaatcgtga
     7381 aaacgctcgt cgccgtgcat aattacgatt aaaattttgt ctttggtatg ttcgaaaatc
     7441 gcgttgacga cggtttcctt actgccgaca tctactccag ccagcggttc gtcaaagata
     7501 taaatgtccg aatctttaac caatcccatc gttatttcca atttttttct ttgaccggcg
     7561 gaaagctcga ccgggtcttt ttcaagatgt ttatcaagcc cgaattccgc agccagccgg
     7621 gcaaactttc ttttttgcgc gtcggaagcg gcaaaactga gattgtccgt taccgagccc
     7681 ggtataaagt caaacggatg aattaccgcg ctgacttttg ccagcggaaa cgtcgtcagc
     7741 aaacccgaag acggcgacaa caatcctgaa acgagatgag cgaacgtgct tttgccgctg
     7801 ccgttcggac cgagcaccaa aatcttttcg ccttcgccgg agcgaaaatc gagctcctgt
     7861 aaaatagagc cctc
//
LOCUS       DASWNE010000011         1660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_671551, whole genome shotgun sequence.
ACCESSION   DASWNE010000011 DASWNE010000000
VERSION     DASWNE010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671551"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1374
                     /locus_tag="VGB68_00305"
     CDS             <1..1374
                     /locus_tag="VGB68_00305"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEY0047695.1"
                     /translation="HPEDLEVHYLKTPLVEEMAILGVKDETAQHAGSEKLVAVIVPDF
                     AYLKANNIANSREAIRHEFDNLGRELPEYQRVRDYIIRVEPLPRTATRKIRRFELQKE
                     IESGAITTKQARDPNQWKLSAEDEKLLDSNAGKAVVTAIKRNAKDTETIHPAMNLEID
                     LGLDSLSRAEVFAALEQAFGIEFGGDEAAKALTVTDAVELVRKHAGEAETTTDISADL
                     NWSKIVKEGETDLPEVRYVLQNRPIFGFFAWSVYKGFNLFFKVFTRLEVRGIENLRRL
                     QRPFLVCPNHQSFLDPFAVCSTYSLDIFRNTFHVGASEFFQNALMRWLSRLLNVVPID
                     PDTQLMRAMKAGAAGLKSGKVLNIYPEGERAFDGNLHEFKKGAAILATELDLPILPVA
                     LDGLHKVWARASWRIRPAKVKIRFGEPFYARDIVKAEMTDEQRYEAVTKHLKDTITAM
                     IAEMRAS"
BASE COUNT          423 a          433 c          437 g          367 t
ORIGIN      
        1 catccggaag atttggaagt gcattatctg aaaacgccgc tggtcgaaga aatggctatc
       61 ttaggcgtca aagacgaaac ggcgcagcac gccgggtcgg aaaagctcgt cgccgtaatc
      121 gtgcccgatt tcgcgtattt aaaagcgaac aatatcgcca actcgcgcga ggcgattcgc
      181 cacgagttcg ataatctcgg acgcgaattg cccgaatacc agcgagtgcg cgattatatt
      241 atccgcgtcg agccgctgcc gcgcacggcg acgcgcaaga ttcgacgctt cgagctgcag
      301 aaggaaatcg aatccggcgc gattacgacc aaacaggcgc gcgacccgaa tcaatggaag
      361 ctttccgccg aagacgaaaa gctgctcgat tcaaacgccg gaaaagccgt cgtcacggca
      421 atcaaaagaa acgccaaaga tacggaaacg attcatccgg cgatgaatct ggaaatcgat
      481 ttgggcttgg attcgctgtc gcgcgcggaa gttttcgccg cgctcgaaca ggctttcggc
      541 atcgaattcg gcggcgacga agccgccaaa gccctgaccg tcacggacgc cgtcgagctt
      601 gtccgaaaac acgcgggcga agcggagacg acgaccgaca tctccgccga tttaaactgg
      661 tcgaaaatcg ttaaggaagg cgaaacggat ttgcccgaag tccgctacgt tttgcagaat
      721 cgcccgattt tcggcttttt cgcgtggtcg gtttataaag gcttcaatct tttcttcaag
      781 gtgtttacgc ggctcgaagt gcgcggcatc gagaatctgc gccggttgca gagaccgttt
      841 ctcgtctgtc cgaatcacca gagttttctc gacccgtttg ccgtctgctc gacttattcg
      901 ctcgacattt tccgcaatac gtttcacgtc ggcgcgagcg aatttttcca gaacgccctg
      961 atgcgctggc tttcacgcct gctcaacgtc gtgccgatcg accccgacac gcagctgatg
     1021 cgcgcgatga aagcgggcgc ggcgggcttg aaaagcggca aggtgctgaa catttatccg
     1081 gaaggcgagc gcgccttcga cggtaatctt cacgaattca aaaaaggcgc ggcgattttg
     1141 gcgaccgagc ttgatttgcc gattctgccg gtcgcgctcg acggcttgca caaagtctgg
     1201 gcgcgcgcct cgtggcggat tcgcccggcg aaagtcaaaa tccgtttcgg cgagccgttt
     1261 tacgccagag acattgtcaa agcggaaatg accgacgagc agagatacga agccgtcacc
     1321 aaacatttaa aagacactat cacggcgatg attgccgaga tgcgcgcctc ctgaagaaga
     1381 taaattttaa cgcgaaacag gcaaagggcg cgaaaattaa acagcaggca aacgcaggag
     1441 aaaaatccgg gaatgacagg atttacagag ccttttaaaa taaaaatcat tattcttatc
     1501 tccgcaaatc ctgttgtcgc tgtaaattct tcctttgcgt tgctgtttct tttcgacttt
     1561 gcgttaaata tttcctttta ctcttcggct ttgctttgca caaaaataaa gaaaatcgag
     1621 aaaaattccg atggctgatt gtaaattcta aattgtttaa
//
LOCUS       DASWNE010000012         6135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_650, whole genome shotgun sequence.
ACCESSION   DASWNE010000012 DASWNE010000000
VERSION     DASWNE010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6135
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="VGB68_00310"
     CDS             <1..918
                     /locus_tag="VGB68_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047696.1"
                     /translation="EYTGKLELINEDSVKIKRIEEQLATVQQEMQENFQKFILRVDAL
                     IIEFRDGGVDFLDRSMRLRNINLLRSETKFREEFEREVLEGWRAKLDQTVDESVDWLV
                     RNNMQLWNDTLEYFNTQVRKSQYDSQIIGRVGGQFVYEREEVHARIRREAEDRIQSLD
                     HREECRRVINSSMTALGQSFGLGAGALGLGYVLASIFTTVALDVTGIAAATALFITSF
                     FILPYKRKRAIEEFKSKLENLRKEMGDVLKSKSDDEIKRSVENVRDALAPYIRFVRAE
                     RDKIEARVSILNQILERLKELTREVKNLG"
     gene            1219..2016
                     /locus_tag="VGB68_00315"
     CDS             1219..2016
                     /locus_tag="VGB68_00315"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY0047697.1"
                     /translation="MSALEKGAYSGNAVDSLRAEGVIAGAQTFRKERAPATVHYHENS
                     HLSFVMQGGVSDKRRNREIERLPGELMFFHAGEPHQTVIESFPTKNINLAVEREFLRA
                     NLATEAAVNSAVDKNPNAKFIMLKIYRELISEDVFSDSSIKMLLLNLIHANPETDSEK
                     ARPGWTSIVAQLTRDKWNESLTLKDLSAAAGVHPITVSKHFPKYFSCTFGEYMRRLKI
                     EKSLYFLKASSFSLTEIAAECGFSDQSHFIRTFKQLTGFLPNNYKKI"
     gene            2134..3831
                     /locus_tag="VGB68_00320"
     CDS             2134..3831
                     /locus_tag="VGB68_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY0047698.1"
                     /translation="MKTAKIFILFIVAFITATNAIAQQNVKKIDELIKRYHDYGQFNG
                     SILVAENGRVVYKKGFGTANMEWSIPNGTDTKFRIASITKQFTAALILQLAEEGKIKL
                     DAKLAEYLADYRKDTGEKVTVHQLLNHTSGIPDYTNIGSNPYSAADFVKKHVSGALEF
                     EPGSKYKYNNGGYSILGAIIEKVTGKSYETVLQERIFKPLGMTNSGFERHSPLLEKRA
                     GGYEKTPAGYINAAYIDMSIPYAAGSIYSTVEDLFKWDQSLYEDVILSAESKKLMFTP
                     GLSNYGYGIRISEQSIGKTDRKTKIIWHGGGINGFNSLISRAVDKRQTVIILDNGSHG
                     QYQPMMTNSIIAILNGQPFEPPKKSIAETLYKIAFEKDVASAVAEYRKLKAENSPAFD
                     FSEIELNKLGYQLLGAKRRKDAIEIFKLNVEMFPKSASAYDTLGEAYLADEQKDSALV
                     NYKKAVELDSTDANALLIVKRLEGKEIKIDSSGFDAYVGDYQVTPALILTISKEGDKL
                     FGQLTGQPKLAVEPVSDAQFTIPEVKANITFEKDSAGKVVGLLLTQGTRSARAKKIK"
     gene            4169..4834
                     /locus_tag="VGB68_00325"
     CDS             4169..4834
                     /locus_tag="VGB68_00325"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEY0047699.1"
                     /translation="MSNKFSEKDLITTGAQIRKAERRQPNGLKRWIPFCFYVPTIIAG
                     LAAAIVNGEYALYLIAPSLVIGWFCWGFFEYAFHRFALHYSGKAGIRPPGNETHLAHH
                     RDPKALERLYVPIYEGLPIALAFFLAATFLTGSWQIAAFLYAGFMLGYLFYELLDYEA
                     HHGKARGRVMRYYKKYHLQHHHADENARYGVTSPVFDYLFGTYYIKPKQARKLAKRAS
                     ETI"
     gene            4831..>6135
                     /locus_tag="VGB68_00330"
     CDS             4831..>6135
                     /locus_tag="VGB68_00330"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0047700.1"
                     /translation="MRIAIISTYTHPTRLPIKEKSVMQSSVPELIAGLCPPHAEIEIY
                     NEKEIDVPLDRDWDLVFFSYLHSYYEHTKVLSALFRSRGMKTVAGGRHAGHFPDDCEK
                     YFDSVIIGSPERSVPELIKDFERGQLKSRYQLRSEGAADIKPARYDLIDFSTNRSRLP
                     GIEASRGCPFSCNFCVLTGHESYHFRKVQDVVEEIKFKMSWNKNYFGLLDDWFVFLDN
                     NLGGSPRYLRELCTELIPLKKTWGCALTFNVLRDEETVKLMARAGCRYIYTGLESLNP
                     ESIKAMNKGQNKVKDIDDVIRRCFKNGILLSFGLLVGSDGDTNEYLEKLPQYLSDLQY
                     FSITFLGIVCPYPETPFYRLLAKEGRLLPKTISRDYDGYTLCHRPLNLEPSEVIHHFK
                     KLAKQLGSFRNVAQHYRSKLFMSDMPNYRRTILGSGLEIKSIR"
BASE COUNT         1680 a         1492 c         1452 g         1511 t
ORIGIN      
        1 gaatataccg gaaagctgga gctcattaac gaagattccg tcaaaatcaa acggatagaa
       61 gaacagctcg ccacggttca acaggaaatg caggaaaatt tccagaagtt tatcctgcgc
      121 gtcgatgctt taataatcga gtttcgcgac ggcggagttg attttctcga ccgcagtatg
      181 cggctgcgga atataaatct attacgcagc gagacgaagt ttcgcgaaga attcgagcgc
      241 gaagtgctcg aaggctggcg cgccaaactg gaccagaccg tcgacgaatc ggtggactgg
      301 ctggttcgca acaatatgca gctgtggaac gacacgctcg aatatttcaa cacgcaggtg
      361 cgtaaatccc agtacgattc gcaaataatc ggtcgcgtcg gcggtcagtt cgtttacgag
      421 cgcgaggaag tgcacgcgcg tatccggcgc gaagccgaag accgcatcca atcgctcgac
      481 cacagagaag aatgccgccg cgtcatcaac agctcgatga ccgcgctcgg gcaatctttc
      541 gggctcggag cgggagcttt ggggctggga tatgtattgg cttcaatttt tacgaccgtc
      601 gcgctcgacg tgaccgggat tgccgccgcg acggcgcttt tcattaccag cttttttatt
      661 ctcccgtaca aacgaaagcg cgccatcgaa gaatttaaat caaaactgga aaatcttcgc
      721 aaagaaatgg gtgatgtttt gaaaagcaaa tccgatgatg agattaaacg ctcggtggaa
      781 aacgtgcgcg acgcgttagc gccgtatatc aggttcgttc gcgcggaacg agataaaata
      841 gaagcgcgcg tttctattct caatcaaatt ctcgaacgcc ttaaagagct gacgcgcgaa
      901 gtcaaaaacc tcggttaaat aaaatgaccg ctcgagaaaa tttcccgaaa actatgagcg
      961 ccggaaaaca ctgtaactgc cggtaattca ccgccgcaag attgcgccga aaagtcaatc
     1021 aactcgaatt caggatttga attttatata tttgaaggaa caaaaatgcg cccggctgcg
     1081 taatgccgat tggtcggaca tttcgcagat gccggatttg aattttaact cgattgagag
     1141 ttccgcgaga ttattcctga tttcatttta ttggaatttg ccgtgaaagc ttttttcgag
     1201 ctttaaagat ttaccataat gtcagcttta gaaaaagggg cgtattcagg aaacgcggtt
     1261 gattcgttgc gggctgaagg cgtaattgcc ggcgcgcaga catttcgaaa ggaacgcgcg
     1321 ccggcaaccg tccattatca cgaaaactca cacctgagct ttgtgatgca gggcggcgtt
     1381 tcggacaaac gcaggaaccg ggaaatcgaa cgactgcccg gagagctgat gttctttcac
     1441 gcgggcgagc cgcatcaaac cgtcatcgaa tcgtttccta caaaaaacat taacttagcc
     1501 gtcgagcggg aatttttgcg agccaatctg gctacggaag ccgccgtcaa ttcggcggtc
     1561 gataaaaatc cgaacgcgaa atttatcatg ctcaaaatct acagggaatt gatttccgaa
     1621 gacgtctttt cggattcttc gataaagatg ctgcttctta atctgattca cgcaaatccc
     1681 gaaaccgatt ccgaaaaagc tcgtccgggc tggacgagta tcgtcgcgca attaacccgc
     1741 gacaaatgga acgaatcgct cactctaaaa gatttatccg cggcggcggg cgttcatccg
     1801 ataaccgtat ccaagcattt tccgaaatat ttttcctgca ctttcggcga atatatgcgc
     1861 agattaaaaa tcgaaaaatc cctttatttc ctgaaagctt cttctttttc tctgactgaa
     1921 atcgcggctg agtgcggttt ttcagaccag agccatttta ttcgcacctt caaacaactt
     1981 accggatttc tgccgaataa ctataaaaaa atatagtcat tactcaaaat tatccgccgg
     2041 aagcaaactt cattctattt ccgccgattt aatttcgata atctgatttg gaaacgtaaa
     2101 acgtttaatc cagatttcat cggagaaatt agaatgaaaa ccgcaaaaat atttatttta
     2161 tttatcgttg cgttcataac cgcgacaaac gcaatcgcac agcaaaacgt gaagaaaatt
     2221 gatgaattaa taaagcgata ccacgactac gggcaattta acggctcgat tctagtcgcc
     2281 gaaaacggca gggtcgtcta caaaaaaggc ttcggaacgg cgaatatgga atggtcgatt
     2341 ccgaacggca cggacacaaa attccgtatc gcatccatca ccaagcaatt taccgccgcg
     2401 ctcattttgc agctcgccga agaaggcaaa atcaagcttg acgcaaagct cgccgaatat
     2461 ctcgccgatt accggaaaga tacgggcgaa aaagttaccg ttcatcaact tcttaatcac
     2521 acttccggga ttccggacta tacgaatatc ggcagcaatc cgtacagtgc cgcggatttc
     2581 gtcaaaaagc acgtcagcgg cgcgctcgaa ttcgagcccg gctcgaaata caaatacaat
     2641 aacggcggct attccattct cggcgcaatt atcgaaaaag ttaccggaaa gtcttacgag
     2701 acggttttgc aggagcgcat tttcaaaccg ctcggaatga caaattcagg atttgagcgt
     2761 cactcgccgc ttctcgaaaa gcgcgccggc ggttatgaaa aaacgcctgc cggttatata
     2821 aatgccgcgt acatcgatat gtcgattccc tacgcggcgg gctcgatata ttcgaccgtc
     2881 gaagaccttt ttaaatggga tcagtcgctt tatgaagacg tgattctttc ggcggaaagc
     2941 aagaaactga tgtttacgcc ggggctttca aattacggtt acggaattcg catttccgaa
     3001 caatcgatcg gaaaaacaga ccggaaaaca aagatcatct ggcacggcgg aggaatcaac
     3061 ggttttaatt ccttgatttc gagagcggtc gataaaaggc agaccgtaat cattctcgat
     3121 aacggcagtc acggacaata tcagccgatg atgacgaatt ccatcatcgc tattcttaac
     3181 gggcagccgt ttgagccgcc gaaaaaatcg atcgccgaaa cgctttacaa aatagcgttt
     3241 gaaaaagacg tcgcgtcagc cgtcgccgaa taccggaagc tgaaagcgga aaattctccg
     3301 gcgtttgatt tctcggaaat tgagctcaat aaactcggtt atcagctttt gggcgcgaaa
     3361 cgcaggaaag acgcgattga gatttttaag cttaatgttg aaatgtttcc aaaatcggcg
     3421 agtgcttacg acactctcgg cgaagcgtat ctggcagacg agcaaaagga ttcggcactc
     3481 gttaattaca aaaaagcggt cgagctcgat tcgaccgacg ccaacgcttt gctgatcgta
     3541 aagcggctcg aaggcaaaga aataaaaatc gattcttccg gtttcgatgc ttatgtcggc
     3601 gactatcaag tcactccggc tcttattctg acaatttcca aagaaggaga taaattattc
     3661 gggcagttga cgggacaacc caaacttgcg gtcgaacctg tttcagacgc gcagtttacg
     3721 attcccgagg tcaaggcgaa tattacattt gagaaggatt ctgccggaaa ggtcgtcggg
     3781 ttattgttaa cgcagggcac gcgctcggca cgagctaaga aaataaaata ataaattccg
     3841 ttttcccgat ttcccgcatc gcctgtccgc tgcgcattac ggacgtttct cactttcttc
     3901 atttattttt acctcgaatt ccggaattac gaaaattgag acgatcttga aaggcggtat
     3961 taaattaccg ccttttttcc tttccgccgt ccgccgtttg gctgcttccg ctattttacg
     4021 gaaatttttg tctgactgat acttttattc cgcccgctac gctttacgat tgaaggcgag
     4081 atttcgacgt aaaaatatgc gcggtcgaaa aagagccgat tgctgatgca gaaatcagaa
     4141 aattgataat aactaacgag aaattataat gagtaataag ttttcagaaa aagatttaat
     4201 cacgacaggg gctcaaatca gaaaagccga gcgccgacag ccgaacggct taaagcgctg
     4261 gataccgttt tgcttctatg ttccgacgat tatcgccggg ctcgcggcgg cgattgtaaa
     4321 cggcgaatac gcgctctatt taattgctcc ttcgctggta atcggttggt tttgctgggg
     4381 attttttgaa tatgcttttc accgctttgc cctgcattat tccggcaaag ccggcattcg
     4441 tccgcccgga aatgaaacgc atctggcgca tcaccgggac ccgaaagcgc tcgaacgcct
     4501 gtatgtaccg atttatgaag gattgcccat cgcgctcgcc ttctttctag cggcgacctt
     4561 tttaaccggc agctggcaga tagcggcttt tctctacgcc ggttttatgc tcggctacct
     4621 gttttatgag ctgctcgatt acgaagccca tcacggcaaa gccagaggac gcgtgatgcg
     4681 ttattacaaa aaatatcatt tgcagcatca tcacgccgac gaaaacgcgc gttacggcgt
     4741 cacgtcgccg gtgtttgatt atttgttcgg aacttattac attaagccta aacaggcgcg
     4801 aaagttagcc aaaagagcga gtgaaacaat atgagaattg cgattatttc cacctacacg
     4861 cacccgacgc gtttgccgat aaaagaaaag tccgtgatgc agtcttccgt gccggaattg
     4921 attgccggac tttgcccgcc gcacgcggaa atcgagattt ataacgaaaa agaaatcgac
     4981 gttccgctcg accgcgattg ggatttggtc tttttttcct atctgcattc gtattacgaa
     5041 cacacgaagg ttctttcggc gcttttccgc agccgcggaa tgaaaaccgt tgccggcgga
     5101 cgccacgccg gtcatttccc ggacgattgc gaaaaatatt tcgattcggt tatcatcggc
     5161 agccctgagc ggagcgttcc cgagctcatc aaagattttg aacgcggaca gttaaaatcg
     5221 cgctatcagc ttcggtcgga aggcgcggcg gacattaaac cggctcgcta cgacctgatt
     5281 gatttttcga ctaatcgctc gcgtctgccc ggcatcgaag cttcgcgcgg ctgtcctttt
     5341 tcctgcaatt tctgcgtcct gaccggacac gaatcctacc atttcagaaa agttcaggac
     5401 gttgtcgaag agataaaatt caaaatgagc tggaacaaaa attatttcgg cttgctggac
     5461 gactggttcg tttttctgga caacaatctc ggcggctcgc cgcgctatct gcgcgagctc
     5521 tgcaccgagc tgattccgct caaaaaaacc tggggctgcg ctttgacttt caatgttttg
     5581 cgtgatgaag aaaccgtcaa attgatggcg cgcgccggct gccgctatat ctacaccgga
     5641 ctcgaatcgc ttaatcccga atccatcaag gcgatgaata aaggacaaaa taaagttaag
     5701 gacatcgacg acgtcattcg ccgctgtttc aaaaacggta tccttttgtc cttcggactg
     5761 ctggtcggct ccgacggcga cacgaatgaa tatctcgaaa aactgccgca gtatctttcg
     5821 gatttgcagt atttttccat tactttttta ggcatcgtct gcccgtatcc ggaaacgccg
     5881 ttttatcgcc tgctcgccaa agaaggcaga ttgctgccga aaaccatcag ccgcgattac
     5941 gacggttaca cgctctgtca tcgcccgctc aatctcgaac cgagcgaggt cattcatcac
     6001 tttaaaaagc tggcaaaaca attgggaagc tttagaaacg tcgcgcagca ttaccgcagc
     6061 aagcttttta tgagcgatat gccgaattac cgccgcacga ttttaggctc gggactcgaa
     6121 ataaaaagca tccgc
//
LOCUS       DASWNE010000013         3816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_247829, whole genome shotgun sequence.
ACCESSION   DASWNE010000013 DASWNE010000000
VERSION     DASWNE010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247829"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..90
                     /locus_tag="VGB68_00335"
     CDS             <1..90
                     /locus_tag="VGB68_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_061524768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEY0047701.1"
                     /translation="HLKECEWRFNNRSKNLYLELLKLLRENPL"
     gene            130..708
                     /locus_tag="VGB68_00340"
     CDS             130..708
                     /locus_tag="VGB68_00340"
                     /inference="COORDINATES: protein motif:HMM:NF025426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HEY0047702.1"
                     /translation="MKSLTNHSLKFILPVLFVLAAITAANAQDARLHFEKLNRLEDRA
                     VDVVEVNIDGKLLDLAKRVLNKSDDPNARKAGQAIAGLKGIYVRVFNFANENEYNIAD
                     VDEIRAQLQTPGWERLVNVRSKRNNQKIDIYTMFAGEVMSGVAVVVSESKSVALINVI
                     GPIDIDLLTEMSGKLNIPKIDIERENQEKPKN"
     gene            724..1320
                     /locus_tag="VGB68_00345"
     CDS             724..1320
                     /locus_tag="VGB68_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047703.1"
                     /translation="MFNKKRILSTLAFPLLVFTFAAAPVNAKTNEYKQIVRHLKTKYQ
                     ARKIRIPMLWLARFAVSVARPAGVKSFSLTVFENVKFSRETLDEEMQSAMRNSFGGDW
                     SSILRVRSREGQQAYMYMREDGKNVRITLVTIDKEQAAIIRATFSPERLAEFVENPKL
                     FGVSLNESDKQAGNRTEKPKEDSKPEAKTKEEKKDSSN"
     gene            complement(2026..2934)
                     /locus_tag="VGB68_00350"
     CDS             complement(2026..2934)
                     /locus_tag="VGB68_00350"
                     /inference="COORDINATES: protein motif:HMM:NF016146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HEY0047704.1"
                     /translation="MESNFDDEAGFSDLPEFQAAIAEFQAAAGRPYYDETADEAARAE
                     YYRDIQPDNLNEQKFYEALKRLLTETHTDRKNYKPAVHLYPWVDLHETGSQLRLKSIY
                     SEKEFNAEDLIAEDFRIERRRERLRRILMSEAGFIAINPERQFNLLEAAMPFNCEHVV
                     PQSWFRKKEPMRGDLHHLFACEIPCNSMRGNFPFFDFPDFGNAIKQDCGKSQGNEFEP
                     AGGKGAVARATLYFLLRYPGEINRTSKEYTEDGIRTILKWHRDNPVSLYEKHRNAAIQ
                     EKQGNRNPLIDFPEWAERIDFSQGLG"
     gene            complement(3024..>3816)
                     /locus_tag="VGB68_00355"
     CDS             complement(3024..>3816)
                     /locus_tag="VGB68_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047705.1"
                     /translation="ADFEAFRQVIEQETNHLPEVVQREHGAGENNYLNKSLLEDAETG
                     EAGTDTLIIVSLDHFKSGQAAREDEIEAVRQFLRSENSCLFVCPHHFIGDDAETQIAE
                     REHHGDPLVPAQQKIGGFGRSLLKELGIEVENYFGLSPAASEDGSPAPLIINYEADEL
                     GVLGGVRTFNLHPHLPHLEILGQNADARVLARQSINPKAPPHPFVKMGNTAFNALLWV
                     KPNGERKGNIFVCDATMWSSAFGGLESLKTFWRNIARLPVAASQP"
BASE COUNT          982 a          941 c          966 g          927 t
ORIGIN      
        1 cacctgaaag agtgcgaatg gcgttttaat aatcgttcaa agaacttgta tcttgaattg
       61 ttgaagttat tgagagaaaa tcctctctaa acttccctag accctaattt atttatcaga
      121 ggagtcatta tgaaatcatt aaccaatcat tcattaaaat ttattttgcc ggtgcttttc
      181 gtgctggcgg cgattaccgc ggcaaacgcg caggacgcgc gcctgcattt cgaaaagctg
      241 aaccggctcg aagaccgcgc cgtcgatgtc gtcgaagtca atatcgacgg caagcttctg
      301 gatttggcga aacgcgtttt aaataaatcg gacgacccga acgcgcggaa agccgggcag
      361 gcaatcgccg gtttgaaagg catctacgtc agagttttca attttgcgaa cgagaacgaa
      421 tacaacatcg ccgacgtgga cgagattcgc gcgcaactgc aaacgccggg ctgggaacgg
      481 ctggtcaacg tgcgcagcaa aaggaacaat caaaaaatag atatttatac gatgtttgcc
      541 ggcgaggtga tgagcggcgt ggcggtcgtc gtttccgaat cgaaaagcgt cgcgctgatt
      601 aacgtcatcg gtccgatcga catcgatttg ctgacggaaa tgagcggcaa attaaacatt
      661 ccgaaaatcg acatcgaaag agaaaatcag gaaaaaccca aaaactaaaa aggagtgaag
      721 acgatgttta ataaaaaaag gatactatcg acgctcgcct ttccgttgtt ggtcttcact
      781 ttcgccgctg cgccggtaaa cgcgaaaact aacgaataca agcagatagt caggcatttg
      841 aaaacgaaat atcaagccag aaagatcagg attccaatgc tctggctggc gcgtttcgcc
      901 gtcagcgtcg cgcgtccggc gggcgtcaaa agctttagcc tgacggtctt tgaaaacgtg
      961 aaattttcgc gcgagacgct tgacgaagaa atgcagtcgg caatgcgaaa ttctttcggc
     1021 ggcgattgga gctctatctt gcgcgtccgc tcgcgcgaag gtcagcaggc ttatatgtat
     1081 atgcgcgagg acggcaaaaa cgtgcggata acgctcgtca cgattgataa agaacaagcc
     1141 gccatcatcc gcgccacgtt cagcccggaa aggctcgccg aatttgtcga aaatccgaaa
     1201 ctcttcggcg tttctttaaa cgagagcgac aaacaggcgg gcaaccgaac cgaaaagccg
     1261 aaagaagatt ccaagccgga agcaaaaacg aaagaagaga aaaaagattc gtctaattaa
     1321 aagacgggaa acaggcgaaa ggcagaaaca gattcgcgtt acgcggagcg cgtcttttat
     1381 cgattaagaa taacgaatga cgaatgcagg cgacaataaa tctcttaatt cgcaattgat
     1441 aattcgcgat tgataaacga cacgctttat taacgcggcg tttccgccgg aagaattcgg
     1501 tcgagcgttt ttgataattg ctcgccgcgg cgaaatacaa gaagttttga gaatttttca
     1561 tattcctgac ctcgaagaat tgtgggcgga attccgggcg cttgcagaca gttaaacgaa
     1621 agaacagaac aagagtagaa gcgtctttat aatcttgtca acattttttg tcggcgggct
     1681 gttccggcga caaatattcg ccggaagccg cgcgcggttc gtaattcgcc gaagtcacag
     1741 tcggaaaatt ctctaaatcg tagatttagt tgagtttata aaagcgagat gcgagaaatt
     1801 caacctgccg aagcgtttgc ctttgattac gcgaccggca cgccggagcc ggattttaaa
     1861 ataaaatgtt tttgtcggac tgtgagctgc cgaaaatatg ttaccggaga agactggaaa
     1921 atagacgcgc cgccaaaaaa atataaaggc tttttcagcc gcgtccggaa agaaaatttc
     1981 gcgcctgact tttttaagaa aatcaggcgc gttctcgatt ccgaattatc ccaagccctg
     2041 cgaaaaatct atacgctcag cccattccgg aaaatcaatc agcgggttgc gattgccctg
     2101 tttttcctgt atcgcggcgt tgcgatgttt ttcgtagagg ctgaccggat tgtcccggtg
     2161 ccatttcaga atcgtccgga tgccgtcttc ggtgtattct ttcgatgtgc ggttgatttc
     2221 gcccggatag cgcagaagaa aataaagcgt ggcgcgcgcg accgctcctt ttcctccggc
     2281 aggctcaaac tcgtttccct gacttttgcc gcagtcttgt ttaatcgcat tgccaaaatc
     2341 gggaaagtca aaaaaaggaa agttgccgcg catcgaattg cagggaatct cgcaggcgaa
     2401 aagatgatgc aaatcgccgc gcatcggctc ttttttgcga aaccacgatt gcggcacgac
     2461 gtgctcgcag ttaaaaggca tcgccgcttc cagcagatta aactgccgct ccgggtttat
     2521 cgcgataaat ccggcttcgc tcatcaatat ccggcgcagc cgctcgcgcc gccgttcgat
     2581 gcgaaagtct tcggcgatta agtcttcggc attgaattcc ttttccgaat agatgctttt
     2641 cagccgcagc tgcgagccgg tttcgtgcaa atcgacccac ggatagagat gaacggcggg
     2701 cttgtaattt tttctgtcgg tatgagtctc cgtcagcaga cgcttcagcg cttcgtagaa
     2761 tttctgttcg tttaaattgt caggctgaat gtcccggtag tattcggcgc gcgccgcttc
     2821 gtcagccgtc tcatcgtaat aaggtctgcc ggcagccgct tgaaattcgg caatcgccgc
     2881 ttgaaattcc ggcaagtccg aaaaaccggc ttcgtcatca aaattagatt ccatatatta
     2941 ttcctcaatt ttagttttta attttccaaa tcaaattctt ttttataaaa acgaaagccg
     3001 tttgccggca atcgcttcgc gtcttatggc tgcgaagcgg cgacaggcaa acgggcgatg
     3061 tttctccaga atgttttgag gctttccagc ccgccgaagg cggagctcca cattgtcgcg
     3121 tcgcaaacaa aaatatttcc tttccgctcg ccgttcggct tgacccacag caaggcgtta
     3181 aaagccgtgt ttcccatttt tacaaacggg tgcggcggag ctttcggatt aatcgactgg
     3241 cgcgccagaa cgcgcgcgtc ggcgttttga ccgagaattt ccaggtgcgg cagatgcggg
     3301 tgcaggttaa atgtcctgac gcctccgaga acgcccagct cgtccgcctc ataattaatt
     3361 ataagcggcg cgggcgagcc gtcttcgctt gccgccggac tcagaccgaa ataattttcg
     3421 acctcgattc ccaattcctt taataagctt ctgccgaagc cgccgatttt ctgctgcgcg
     3481 ggaaccagcg ggtcgccgtg atgctcgcgc tcggcgattt gcgtttccgc gtcgtcgccg
     3541 ataaagtgat gcgggcagac aaacaggcag gaattttcgc tccgcagaaa ttgcctgacg
     3601 gcttcgattt catcttcgcg cgccgcctgc ccgcttttga aatggtcgag gctgacgatg
     3661 attaaagtgt ccgttccggc ttcgccggtt tccgcgtctt ccagcagcga tttattcaga
     3721 tagttattct cgcccgcgcc gtgttctcgc tggacgactt ccggcagatg attcgtttcc
     3781 tgctcgataa cctgccggaa agcttcaaaa tccgct
//
LOCUS       DASWNE010000014         2804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_141846, whole genome shotgun sequence.
ACCESSION   DASWNE010000014 DASWNE010000000
VERSION     DASWNE010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141846"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="VGB68_00360"
     CDS             complement(<1..890)
                     /locus_tag="VGB68_00360"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEY0047706.1"
                     /translation="MKRRVVVTGLGLVTALGNDVESTWSALVRGESGVDFIKKFDTEK
                     YSVKFAAEVKDFDPLNFLEKKEARRMGAFTHFAIAASDEAIRHSGLVIDDSNAEMVGT
                     YISSGIGDFWAIEREHDKLLLGGPDRVSPFFIPQSIVNLASGNVSIRHGAKGPNTATA
                     TACAAGAHAIGDSFRYIERGDADVMICGGAESAITPMSVAGFASMRALSTRNDEPKKA
                     SRPFDAERDGFVMGEGAGIIILEELEFAKARGAKIYAEIVGYGMSGDAFHITAPDETG
                     SGPRRVMLKAIKDAGISPEQ"
     gene            complement(977..1222)
                     /locus_tag="VGB68_00365"
     CDS             complement(977..1222)
                     /locus_tag="VGB68_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY0047707.1"
                     /translation="MADSAVQEKIKQIIVDELGVDEAEVTENARFIEDLGADSLDLVE
                     LVMRFEEEFGIEIPDEDAEKIQSVRDAYSYVEQHKKD"
     gene            complement(1247..2209)
                     /gene="fabD"
                     /locus_tag="VGB68_00370"
     CDS             complement(1247..2209)
                     /gene="fabD"
                     /locus_tag="VGB68_00370"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEY0047708.1"
                     /translation="MSEESKIENLNSKIAYIFPGQGSQAVGMGKDLFDSFPASREVFQ
                     EADDALGFKLSEMCFAGDEADLQLTANTQPAILTTSVAAFRAMESEGFPSPDYVAGHS
                     LGEYSALVAARAMSFADAVKTVRKRGTYMQEAVPVGVGAMAAVIGLPLETVETACADA
                     AESDVCSPANINSPSQIVIAGNAEAVDRAIVLLKERGAKRAIKLNVSAPFHCALMLPA
                     QAKLAEDLQEINFVDLRFPIIENVSAEANTKGERVRAALTEQVSKPVRWARSVENLIT
                     QGVTTFVEAGAGKILSGLVRQINRDVRCLNVENFDSLKQAVPML"
     gene            complement(2293..>2804)
                     /locus_tag="VGB68_00375"
     CDS             complement(2293..>2804)
                     /locus_tag="VGB68_00375"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEY0047709.1"
                     /translation="FGDGAGAAILGPVPENKGILATKIRSDGRYEEQLYAPGGGTKLG
                     TSYKTIDDGMHFFKMKGNELFKVAVRSMAEVSAEMLEKAGYTVDDVDLVIPHQANQRI
                     TDAVASRLGVPEEKVYSNIAEHGNTSSASIPIALDECIEAGKITEGSLVLLTAFGGGV
                     TWGGTLIRF"
BASE COUNT          616 a          807 c          681 g          700 t
ORIGIN      
        1 atttgttcgg gcgagattcc cgcgtcttta atcgctttca gcatcacgcg gcgcggaccc
       61 gagccggttt cgtccggcgc agtgatgtga aaagcgtcgc cgctcattcc gtaaccgaca
      121 atttcggcgt aaatctttgc gccgcgcgct ttggcaaatt ccagctcttc gagaataatt
      181 atccccgcgc cttcgcccat cacgaaaccg tcgcgctcgg cgtcaaacgg acgcgaagct
      241 tttttcggct cgtcgtttct ggtcgataag gcgcgcatcg aagcgaatcc ggcgaccgac
      301 atcggcgtaa ttgccgattc cgctccgccg caaatcatca cgtcggcgtc gccgcgctcg
      361 atataacgga agctgtcgcc gatggcgtgc gcgcccgccg cgcaggcggt cgccgtcgcc
      421 gtgttcggac cttttgcgcc gtgacgaatc gagacgttgc cggacgcgag attgacgatt
      481 gactgcggaa taaaaaacgg cgaaacgcgg tcgggaccgc cgagcagaag cttatcgtgc
      541 tcacgctcga tcgcccagaa atcgccgatg cccgaactga tataagttcc gaccatttcg
      601 gcgtttgaat cgtcaatcac cagaccgctg tgcctgatgg cttcgtcgga ggcggcgatg
      661 gcaaaatgcg taaacgcgcc catccgccgc gcttctttct tttccagaaa gttgagcggg
      721 tcgaaatcct ttacttcggc ggcaaatttg accgaatact tttcggtatc gaattttttg
      781 atgaagtcaa cgccgctttc gccgcgaacg agtgccgacc aagtcgattc gacatcgttt
      841 ccgagcgccg tgactaaacc gagtcctgtt actacaacac gacgtttcat aaaatttagt
      901 ggtaagcggt aaacggtaag tcgtaagcga ttgtttttat cttttcactt acgacttacc
      961 gtttaccgct taaaaattag tctttcttat gctgctcgac gtagctgtaa gcatcgcgga
     1021 cgctttgaat tttttccgcg tcttcgtcgg gaatttcaat accgaattct tcttcgaaac
     1081 gcatcacgag ctcaactaaa tcgagcgagt ctgcgcccaa atcttcgata aaacgggcgt
     1141 tttcggtaac ttccgcttca tccaccccta gttcgtcaac gataatctgt ttgatttttt
     1201 cctgaacggc tgaatctgcc atagcaactc tttctcctgt taaaatttat agcattggta
     1261 ctgcttgttt taaactatca aaattttcga cgttcaaaca gcgaacatca cggttaattt
     1321 ggcgaaccaa acccgataaa attttccccg cgccggcttc gacaaatgtt gtgacgcctt
     1381 gcgttatcaa attttccacc gaccgcgccc agcggaccgg ttttgaaacc tgttcggtca
     1441 gagcggcgcg aactctttcg cctttcgtat tggcttcggc ggaaacgttt tcgataatcg
     1501 gaaatcttaa atcgacgaaa ttaatttcct gtaaatcttc tgccagcttt gcctgcgccg
     1561 gaagcatcag agcgcaatga aaaggcgcgg aaacattcaa ttttatcgcc cgtttcgcgc
     1621 cgcgctcctt taacaataca attgccctgt cgaccgcttc ggcgtttccg gcaatcacga
     1681 tttgcgacgg cgaattgata tttgccggac tgcaaacgtc gctttcggcg gcgtcggcgc
     1741 aagccgtttc gaccgtctcg agcggcaagc cgataaccgc cgccatcgcg ccgacgccga
     1801 ccggaaccgc ttcctgcata tatgttccgc gtttgcgaac cgtttttacg gcatcggcaa
     1861 agctcatcgc gcgcgccgcg accagcgccg aatattcgcc caaactgtgt ccggcgacgt
     1921 aatccggcga cgggaaacct tccgattcca tcgcgcgaaa agcggcgacg gaagtcgtca
     1981 aaatcgccgg ctgcgtattt gccgtcagct gcaaatcggc ttcgtcgccc gcaaaacaca
     2041 tctcggacaa tttaaaaccg agcgcgtcgt cggcttcctg aaaaacttcg cgggacgccg
     2101 gaaaactatc aaataaatcc ttgcccattc cgaccgcctg cgagccttgt ccgggaaata
     2161 tataggcgat tttggagttt agattttcga ttttcgattc ttcgctcatt cgagtctcgt
     2221 tttaacttta atttacaatt attcggattt gactggatgg attaaccagg cgaccagtcc
     2281 agaatccaga atctaaaatc taatcaaggt tccgccccaa gtgacgccgc cgccgaacgc
     2341 cgtcagcaat accagactgc cttcagtgat ttttccggct tcgatgcatt cgtccagcgc
     2401 aatcggaatc gaagccgacg atgtgttgcc gtgttcggcg atgttcgaat aaaccttttc
     2461 ttccggcacg cccagacgcg aagcgacggc gtcggtgatg cgctgattag cctgatgcgg
     2521 aatcaccaga tcgacgtcgt caaccgtata gcccgctttt tccagcatct ccgccgaaac
     2581 ttcagccatc gagcgcaccg cgactttgaa aagctcgttg cctttcatct taaaaaagtg
     2641 catcccgtcg tcgatggttt tgtagctcgt tcccagtttc gtgccgccgc cgggcgcgta
     2701 aagctgttct tcgtagcgac cgtcggaacg aattttcgtg gctagaatcc ctttgttttc
     2761 cggaaccgga ccgagaatcg ccgcgcccgc gccgtcgccg aaca
//
LOCUS       DASWNE010000015         4007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_601025, whole genome shotgun sequence.
ACCESSION   DASWNE010000015 DASWNE010000000
VERSION     DASWNE010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601025"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..959)
                     /locus_tag="VGB68_00380"
     CDS             complement(96..959)
                     /locus_tag="VGB68_00380"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsnF/AvaK domain-containing protein"
                     /protein_id="HEY0047710.1"
                     /translation="MTHTVIGLFDNKNEARVAMEELIEQGFIRENIDVSDRRYDDSTA
                     STTTDTTSGTGVGDSIGNFFSSLFGDDQTTAQNYTTLAQEADAILTVQVDTEDRARAA
                     AEILDRHGAIDLDERSAQYQQNYAQTSGTTQNTATQNTATQNTATTTEGEMSIPVIEE
                     ELQVGKRAVERGGARIRSRIVEKPVEETLRLREEHIVVNRHPVNRAVTDADLQHVQEG
                     DIELTERAEEAVVSKQARVVEEVSVGKQVSEREETVRDTVRRTDVEVEETGGSVTRET
                     DDNINTRKANS"
     gene            1351..2250
                     /locus_tag="VGB68_00385"
     CDS             1351..2250
                     /locus_tag="VGB68_00385"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEY0047711.1"
                     /translation="MSRDFLEDYKAIREGGIGFREQKRGLIEVSGREAVQFLNGLITN
                     DVAALEDGTQMSAAFPNAQGRLLAFVRVRRKGDKFFFETAETTHSKVFQNLYRFTFAG
                     DFFVEDRSENYRYFEILNSGFQISNPDSIVFESKSGEDFFVPKDAAENFGNELLKASA
                     VEISDELSEVLRIENGTPLYGVDMDETTVVLETGLDEAVSFKKGCYIGQEIIARIHFR
                     GHVAKKLTGLIFDAENASINPNDEIKSTEGKNAGRVTSTAFSPKLEKTIALAFVRYDY
                     LAEGTELKVNDLTAKVKNLPFVR"
     gene            2391..2648
                     /locus_tag="VGB68_00390"
     CDS             2391..2648
                     /locus_tag="VGB68_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047712.1"
                     /translation="MSDLDYPNAKLAYTIIQSLLRNQEALSDLLALMAHALDEDVAKA
                     LTNTGEWEKYLEAKRELETTKLQIEKFTAELSKIENENNIG"
     gene            2702..3634
                     /locus_tag="VGB68_00395"
     CDS             2702..3634
                     /locus_tag="VGB68_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047713.1"
                     /translation="MRLQTFGGLFLLIFLFLFPAARAQGQQAENPYAEGTTQTAIFDR
                     FPALPECDLRGRLDNFLVALRDNPSAKGYMIFYRGADEAPAQQTDVFAGRVLNLYANH
                     VRFRRFPPERIITIDGGFRATRMTELWFVPPSGEIPQPSDTVEKPEQPKNKALMVESN
                     YLELTGAEMPEPEESAEETGVQQEAVENQEAKNEVAEIESEAETEPQEEFFEWTSGYF
                     AELLNNDKSVKGRVIFYLDETEYDLAKARAIVERGMRKSAKETETDLSRVKIIFGGYR
                     ESPEIEFWAVPRGAKKPKPTPQKRKIKEELGNEN"
     gene            3706..>4007
                     /locus_tag="VGB68_00400"
     CDS             3706..>4007
                     /locus_tag="VGB68_00400"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0047714.1"
                     /translation="MLDLIIIGAGPAGISAAYEAQKLGLNYLVIEKGLIGNTIYNYPI
                     GLAVFSTVNELELIENTLKPAREKPTREELLSYYVLFVLENNLRVQTEEEVLTVEKP"
BASE COUNT         1023 a          947 c         1023 g         1014 t
ORIGIN      
        1 cgcgcacctt cacgtttcac gtttccgcgc gccgtcaaat aaaaagcgaa tctcgtttaa
       61 agattcgcct ttccgtaaat tcattatcct gaattttagc tgtttgcctt gcgggtattg
      121 atattatcgt cggtctcgcg cgtgacgctt ccgccggttt cttcgacctc gacgtccgtg
      181 cggcgcaccg tatcgcggac ggtttcttcg cgttccgaaa cctgtttgcc gaccgaaact
      241 tcttcgacga cgcgagcctg tttggaaacg accgcttctt cggcgcgttc cgtcaattcg
      301 atgtcgcctt cctgtacgtg ctgcaaatcg gcgtcggtga cggcgcgatt taccgggtgt
      361 cgattaacga cgatgtgctc ttcgcgaaga cgcagagttt cttcgaccgg tttttcgacg
      421 atgcggctgc ggatgcgcgc tccgccgcgc tcgactgcgc gtttaccgac ctgcaattct
      481 tcctcgataa ccggaatcga catttcacct tcggtcgtcg tcgcggtatt ttgcgtcgca
      541 gtattttgcg tcgcggtgtt ttgcgtcgtg cccgatgttt gagcgtaatt ttgttgatat
      601 tgcgccgagc gctcgtcaag gtcgatggcg ccgtgacggt cgagaatttc agccgccgct
      661 ctggctctgt cctccgtatc gacctgaacg gtcaatatcg cgtcggcttc ctgcgcgagt
      721 gtcgtgtaat tttgcgcggt cgtctggtcg tcgccgaaca gcgagctgaa aaaattgccg
      781 atgctgtcgc cgacgcccgt gcccgatgtc gtatcggttg tcgtgctcgc cgtcgaatcg
      841 tcgtatcttc tgtccgaaac gtcgatattt tcacgaataa aaccctgctc gatcagttcc
      901 tccatcgcca cgcgggcttc gtttttattg tcgaacaaac ctataactgt atgtgtcata
      961 atacctcctt gaatataacg ccgaatttat cggcaatccc cttttgatta accgccgaaa
     1021 attcgctttt cggcggcgtg tgcttaacgc cgttttcgtt tttaacggct gtctttttct
     1081 ctgaacaata gaacgcttct tttgttcgcg ctgagttgaa aaactatcgg gctttcagca
     1141 actggcgttc cacttttgaa caaacacccg tctataaata attttaaatt tataagcggt
     1201 ttatttattc aaaagaaaaa ctgaaaatat ttcgccggag tgctgtgtta aaatcgaacc
     1261 tgaacattgt ttgtattgaa ttgattcttt gtgccagtat cgaatacagg cacgaggttt
     1321 cggaaggcga ttttgaacgg ataaaacgta atgagcagag attttttgga agattataaa
     1381 gctatccgcg aaggcggaat cggatttcgc gagcaaaagc gcggattgat tgaggtttcg
     1441 ggcagggaag ccgtccagtt tttgaacggc ttgattacga atgacgtcgc cgcgctcgaa
     1501 gacggcacgc aaatgtcagc cgcttttccg aacgctcagg gacgactcct ggctttcgtc
     1561 cgcgttcggc gcaaaggcga caaatttttt tttgaaaccg ccgaaacgac gcactcgaag
     1621 gttttccaaa acctttatcg ctttactttt gccggagatt ttttcgtcga agaccgctcg
     1681 gaaaattaca ggtattttga aattttaaat tccggatttc agatttcaaa tccggattca
     1741 atcgttttcg agtcaaaatc aggcgaagac tttttcgtgc cgaaagatgc ggcggaaaat
     1801 ttcggaaacg aattgctgaa agcaagcgcc gtcgagattt ccgacgagct ttcggaagtt
     1861 ttgcgcatcg aaaacggcac gccgctctac ggcgtcgata tggacgagac gacggtcgtg
     1921 ctggaaaccg gtctggacga agcggtcagc tttaaaaaag gctgttacat cggtcaggaa
     1981 attatcgccc gcattcattt tcgcggtcac gtcgccaaaa aactgaccgg attgatattc
     2041 gatgcggaaa acgcgtcgat aaatccgaat gacgagataa aatcgaccga aggcaaaaac
     2101 gccggtcgcg tgacatcgac ggcattctcg ccgaagctcg aaaaaacaat cgcgcttgcc
     2161 tttgttcgct acgattacct ggcggaagga accgagttga aagtaaacga tttaacggcg
     2221 aaagtgaaga atttaccgtt tgtccggtag atagtaaaat ggtttgagag gattaagaaa
     2281 atgtcccgcg cgaatcggtc gaaaacggcg cgcggctgat tcccgcagaa tcgaacgcga
     2341 ggaaaataaa acgcaggatt aatttataat cgaacgcagc acagataaat atgagtgatt
     2401 tagattaccc gaacgccaaa ctcgcctaca cgattatcca atcgcttttg cgaaatcagg
     2461 aagctttgag cgatttgctc gccctgatgg cgcacgcgct cgacgaagat gtcgccaaag
     2521 ccctgaccaa cacgggcgaa tgggagaaat atctggaagc caagcgcgag ctggaaacga
     2581 cgaaattgca gattgaaaaa tttacggcgg agctttccaa aattgagaac gaaaataata
     2641 ttggttaatc gcgcgcgtta agctaaaata ctcggaagct tcggctcccg gcagagaaat
     2701 tatgcgctta caaactttcg gcggactatt tttactgatt tttcttttcc tgtttccggc
     2761 ggctcgcgcg cagggacaac aggctgaaaa tccatacgcg gaaggaacga cgcaaacggc
     2821 gatatttgac cgttttccgg cgctgcccga atgcgatttg cgcggcaggc tcgacaattt
     2881 tctggtcgct ctgcgcgata atccttcggc gaaaggttat atgatttttt atcgcggcgc
     2941 ggatgaagct ccggcgcagc agacggacgt ttttgccggt cgtgttttga atttgtatgc
     3001 aaatcacgtg cgctttcgcc gctttccgcc ggaaagaata attacaatcg acggcggttt
     3061 tcgtgcgacg agaatgacgg aattgtggtt tgtgccgccg agcggtgaaa ttccgcagcc
     3121 ttccgacaca gtcgaaaagc ccgaacagcc gaaaaacaag gctttgatgg tcgaaagtaa
     3181 ttatctggaa ttgacaggcg cggagatgcc cgagcctgaa gaatccgccg aagaaaccgg
     3241 agttcagcag gaagcggtcg aaaatcagga agcgaaaaat gaggtcgccg agattgaaag
     3301 cgaagcggaa accgagccgc aggaagaatt ttttgaatgg acttccggtt atttcgcgga
     3361 gcttttaaat aatgataaat ccgtcaaagg gcgcgtaatt ttctatctcg atgaaaccga
     3421 atacgatttg gcaaaggcgc gcgccattgt cgaaagagga atgcggaagt cggcaaaaga
     3481 aaccgagact gatttgagcc gcgtgaagat aattttcggc ggttatcgcg aaagtccgga
     3541 aatcgaattc tgggctgtcc cgcgcggcgc gaaaaagccg aaaccgacgc cgcagaaacg
     3601 aaaaatcaag gaagaattag gaaatgaaaa ctgagagata agaattgaaa agcttttgaa
     3661 ccttttagtt ctcacttctc agctctcatt tctcatttat aaagaatgtt agatttgata
     3721 ataatcggcg ccggtcccgc cggaatttcc gccgcttacg aagcgcaaaa gctcggtctg
     3781 aattatctcg tcatcgaaaa aggcttaatc ggcaacacga tttacaatta cccgattgga
     3841 ctggcggttt tttcgaccgt caacgaattg gaattaatcg agaacactct gaaaccggcg
     3901 cgcgaaaagc cgacgcgcga ggagcttctg tcttattacg tgcttttcgt gctggaaaat
     3961 aacctgcgtg tccagaccga agaagaagtt ttaacggtcg aaaaacc
//
LOCUS       DASWNE010000016         2541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_247870, whole genome shotgun sequence.
ACCESSION   DASWNE010000016 DASWNE010000000
VERSION     DASWNE010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247870"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..747)
                     /locus_tag="VGB68_00405"
     CDS             complement(154..747)
                     /locus_tag="VGB68_00405"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0047715.1"
                     /translation="MIKILTCLLVVVLAALAADGQTVNKTKCELQELAIEAALGNALA
                     QYNMGVEFFRGEQVAQDFGKAATMWRLAGAGGQISAYNNLGYLTYYGRGVKQDHAEGV
                     RLWRIAAEAGIAESQIHIGYAYLKGDFLKKDAVEAYAWATTGKHFVRKVEDKILARAT
                     DEMANKLLTQARSFLNAEQIAEAEKRAAAYIAKFSTK"
     gene            complement(838..1377)
                     /locus_tag="VGB68_00410"
     CDS             complement(838..1377)
                     /locus_tag="VGB68_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047716.1"
                     /translation="MKRIFSIFAFSAALSVVFALSAFGQNLTFSDLNAPYTFDLPEAK
                     WKQTVKPSAGSPTVEYVYGDRRDGHLEIRKITIAANEFLSDVILREQEQKLQFLPGYV
                     AGKEENFAGALKGKVFNFEYVRSGRNISGRFYFLKSDDTTLYIVRFTGERDKLKAIRN
                     QTDSIARTFKLKSQTANNN"
     gene            1663..>2541
                     /locus_tag="VGB68_00415"
     CDS             1663..>2541
                     /locus_tag="VGB68_00415"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HEY0047717.1"
                     /translation="MKRNFSLFSRFTALVLLLALASPLFAVVGDTKDKRKALTEDQKI
                     LHVLNRLGFGARPGDVEKVKAVGLQKYIEQQLNPSTINDAALEAKLKRFEILDMTTAQ
                     IFARYPNGNALLRMLQGGRRNAQNRAQNNQNQAQNNQMPNADRQPPTEITEADRKARQ
                     EKLRALYEQYDLRPANQIMQQQISARVLRGVYSERQLQEVMTDFWANHFNVYSGKAAV
                     RWYIPSYERDVIRKNALGSFKDLLAGTAQHPAMLFYLDNFQSVSPNAQGANNRAGGVL
                     QRAAQNGQLNERQRERL"
BASE COUNT          632 a          633 c          638 g          638 t
ORIGIN      
        1 cgcatcatca cgtctctgcc ggaaagcgaa acgcgcccga tgaagcttca aaaggcgcat
       61 tctttagacg aaattttgtc gggaattgat gaaagcgcga aaggatatat actgcctttc
      121 ggcgcaaagt ttttgattaa agcgaaaagc tgattatttt gtagaaaatt tggcgatata
      181 tgcagccgct cttttttccg cttcagctat ctgctcggcg tttaaaaaac tgcgcgcctg
      241 agtcaaaagt ttgttcgcca tctcgtcagt ggctcttgcc agaattttgt cttccacctt
      301 tcgcacaaaa tgtttgccgg ttgtcgccca ggcgtatgct tcgaccgcgt cttttttcag
      361 gaaatcgcct ttcaaataag cgtagccgat gtgaatctgc gactcggcaa ttccggcttc
      421 cgccgcgata cgccataatc tgacgccttc ggcatgatcc tgtttgacgc ctctgccgta
      481 ataagtcagg tagccgagat tgttatacgc ggaaatttgt ccgcccgcgc ccgccagacg
      541 ccacatcgtc gccgctttgc cgaaatcctg cgccacctgc tcgccgcgaa aaaactcgac
      601 tcccatattg tattgagcca gcgcgtttcc caaagccgcc tcaatcgcca gttcctgcaa
      661 ttcgcatttg gttttattga ctgtctgacc gtctgccgca agcgccgcaa ggacgacaac
      721 caaaagacag gttaatattt taatcatctt attttccaat agataaccgg cgcgacaata
      781 gaacgtccgg caatgtttcc aagccgctta tcgctcgttc agattgtaaa aaaaagttta
      841 gttgttgttt gccgtttgcg atttcagctt gaaagttcgg gcaatcgaat cggtctggtt
      901 gcggatggct ttcagtttat cgcgctcgcc cgtaaagcgg acgatgtaaa gcgtcgtgtc
      961 gtcggatttc agaaaataaa atctgccgct gatgtttctg cccgagcgaa cgtattcaaa
     1021 attaaaaacc ttgcctttta aagcgccggc aaaattttct tcctttccgg cgacgtagcc
     1081 gggcagaaat tgcagcttct gttcctgctc gcgcaatata acgtcggaga gaaattcgtt
     1141 cgccgcaatc gtgattttgc ggatttccag atgcccgtcg cggcggtcgc cgtagacgta
     1201 ttcgaccgtt gggctgccgg cgctcggctt gacggtctgt ttccatttcg cttcgggcaa
     1261 atcgaaggtg taaggagcgt ttaaatcgct gaaagtcagg ttttgcccga aagcggacaa
     1321 ggcgaaaact acacttaaag cggcggagaa tgcgaaaata gaaaaaatgc gtttcattat
     1381 gtttaattcc tcgggcggta tcgaaatcgt tttaatatta acttaaaatt cggaaaaata
     1441 cgatttttaa gatgagagaa cgttcgcggt tcgttgcttt tcctttagga ttcaggattt
     1501 aggattcaag attcaggatt caagatttgt aattttgaat cttgaatcct gaatctcgaa
     1561 tcttgaatct taaaggaaat ttcttcggct tttccgcata gcggcgcatc taaatttgca
     1621 ggtaagactg aacaggtcca taaagttccg aggttaaaga taatgaaacg aaacttctcc
     1681 ttattttcca gattcaccgc gctcgtgctt cttctggcgc tggcgagtcc gcttttcgcg
     1741 gtcgtcggcg acacgaaaga caagcgcaag gcgctgaccg aagaccagaa aattctgcac
     1801 gttctgaacc ggctcggttt cggcgcgcgt cccggcgatg tcgaaaaagt aaaagccgtc
     1861 ggtttgcaga aatacatcga gcagcagctg aatccttcga ccattaacga tgcggcgctg
     1921 gaagcgaagc tgaaaagatt tgaaattctc gatatgacga ccgcccagat tttcgctaga
     1981 tatccgaatg gaaacgcttt gctgcgaatg ctccagggcg gacggcgcaa cgcgcagaat
     2041 cgagcgcaga acaatcaaaa tcaggcgcag aacaatcaaa tgccgaacgc cgaccggcag
     2101 ccgccgacgg aaataacgga agccgaccgc aaggcgcggc aggaaaagct gcgcgcgctt
     2161 tacgagcagt acgatttgcg tccggcaaac cagattatgc agcagcagat ttccgcgcga
     2221 gttctgcgcg gcgtttattc cgagcgccag ctgcaggaag tgatgacgga tttctgggcg
     2281 aatcatttca acgtttattc gggcaaagcc gccgtgcgct ggtatattcc gtcgtatgaa
     2341 cgcgacgtga ttcgcaaaaa cgctctggga agttttaaag atttgctcgc cggcacggcg
     2401 cagcatccgg cgatgctttt ttatctcgat aatttccaat ccgtttcgcc gaacgcgcaa
     2461 ggcgcaaaca atcgcgcggg cggcgttctg cagcgcgccg cgcagaacgg gcagttaaac
     2521 gagcgccagc gcgaaagact g
//
LOCUS       DASWNE010000017         1649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_530148, whole genome shotgun sequence.
ACCESSION   DASWNE010000017 DASWNE010000000
VERSION     DASWNE010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530148"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VGB68_00420"
     CDS             <1..193
                     /locus_tag="VGB68_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047718.1"
                     /translation="QKTDEFHCGVLEIDKLTGIFYLNDFSVKDDFPFLLDDFKYYGKV
                     VGYCLLEEDSGTQILFRPF"
     regulatory      complement(387..555)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            complement(601..1137)
                     /locus_tag="VGB68_00425"
     CDS             complement(601..1137)
                     /locus_tag="VGB68_00425"
                     /inference="COORDINATES: protein motif:HMM:NF016153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HEY0047719.1"
                     /translation="MINTAARQEKKSLLLINLLSVVVPLAVAILLSFPNKLALGAWTK
                     NLSHAIGIVNTLTSILLIFGLILIRRNKPELHRLVMSAAFVLGGLFLVFYITYHISNP
                     ANRFAGAGFVRYAYLFILISHIGLSLVVLPLVLRAMFFAVTGQFARHKSIVRFAYPIW
                     LYVSISGVIVYFMLYHIY"
     gene            complement(1152..>1649)
                     /locus_tag="VGB68_00430"
     CDS             complement(1152..>1649)
                     /locus_tag="VGB68_00430"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEY0047720.1"
                     /translation="FGGLISAYIVLSTNQDLEWKPFALPWQVWVSTVLIIASSFCYKI
                     AQNNLNKDRQKAAKQWLLATTVLGGTFISSQILLWFQLVKAGVYMQSNPYAGFFYILT
                     AVHALHVLGGICALGYIVLRAWRETNSEEEMVRRKTAATVVGWYWHFMDGLWIVLLLL
                     LGFFK"
BASE COUNT          476 a          389 c          391 g          393 t
ORIGIN      
        1 tcagaaaacc gatgaatttc attgcggagt gttggaaatc gacaagctga ccggaatttt
       61 ttatctgaat gatttttccg tgaaagacga tttcccgttt ctgttagatg attttaaata
      121 ttacgggaag gtcgtcggct attgtttgct tgaagaagac agcggcacac agattctgtt
      181 tcgacccttt taacgggtcg aaagcgcgtt tgcccagagc gcgactttgc gtctttaaac
      241 ttattttcag attaaaagcg aaagctccgt atcacgtgat acggagcttt tttttgagaa
      301 gaccggagtc ttttaggatt tgttgaaaaa taaaaggcgc aaagctgacg aaatcgcacc
      361 tacctgaaac ggcaaaactt tttcgtgacc ttcttcacca tagcttacga ggttagctgc
      421 cgggctagga aagaagaagt ttcccgcgtc aaccatgaaa tgacgctacg cccctgtttc
      481 accttttgga gttagtaaaa ctatttggtt cccccgcgcc gcttcttttg gaagcgactt
      541 cagcgacaac ttttcaactt gaaaaatata gcattttcgc gcgcgtcgcg caagtaaaaa
      601 ttaataaata tgatagagca taaaataaac tatgacgccg ctgatcgaaa cgtacaacca
      661 gatcggataa gcgaatctga cgattgattt gtgccgcgcg aattgcccgg tcacggcgaa
      721 aaacatcgcc cgcaaaacca gcggcagaac gacgagcgac aagccgatgt ggctgattaa
      781 gatgaataga taagcgtagc ggacgaaacc ggcgccggcg aaacgattgg cgggattgga
      841 gatgtgatag gtaatataaa aaaccagaaa taagccgccc agcacgaatg ccgccgacat
      901 tacgaggcgg tgaagctccg gcttatttcg ccgtatcaaa atcaatccga atattaaaag
      961 aatcgaggtt agcgtattga cgatgccgat ggcgtgcgac agatttttcg tccacgcgcc
     1021 caaagccagt ttattcggaa acgaaagcaa aatcgccacc gccagcggca cgacgaccga
     1081 caacagattt atcaaaagca gcgatttttt ttcttgtcga gccgcggtat ttatcattat
     1141 ctatcgggaa tttatttaaa aaatccgagc agcagcagca gcacgatcca aagcccgtcc
     1201 ataaagtgcc aataccagcc gacgaccgtc gcggctgttt tgcgccgaac catttcctct
     1261 tccgaattcg tttcgcgcca ggcgcgcaaa acgatataac cgagcgcgca gattccgccg
     1321 agaacgtgca gggcgtgaac ggcggtcaga atataaaaaa atcccgcata cggattgctc
     1381 tgcatataaa cgccggcttt gacaagctga aaccacaaaa gaatctgcga ggaaataaac
     1441 gttccgccca aaacggtcgt cgccagaagc cattgcttcg ccgctttttg ccggtctttg
     1501 tttaaattat tttgagctat tttgtaacag aagctgctgg cgattatcaa aaccgtgctg
     1561 acccaaacct gccacggcag cgcgaaaggc ttccattcga ggtcttgatt ggttgaaagg
     1621 acgatatacg cgctgattaa gccgccgaa
//
LOCUS       DASWNE010000018         1812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_212496, whole genome shotgun sequence.
ACCESSION   DASWNE010000018 DASWNE010000000
VERSION     DASWNE010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212496"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(452..>1812)
                     /locus_tag="VGB68_00435"
     CDS             complement(452..>1812)
                     /locus_tag="VGB68_00435"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0047721.1"
                     /translation="RQLLSLVFEGEGHSDRTANNGFEALEKLQTEPAEVIISDVKMPD
                     MDGIEMLRAVRGVLPDVGVVMMTAFASVETAREAFKLGADDFIQKPFDVEELKLIVKK
                     TLDKQALIVENRAFKRAQRERGSIKNIIGHSDKMQAVYQMIETVAEVQSTILITGESG
                     TGKELVARAIHDMSPRAEKPFISINCGAFTETLLESELFGYMKGSFTGANANRKGLFE
                     AAHKGTIFLDEIGEMSPAMQVKLLRVLQERRVRPVGAHDELPIDARVIAATNRDLKAM
                     TVDGSFREDLFYRISVIPISLPPLRERREDISSLVSHFIKKYCDQTGRNLSISERAEQ
                     ILENYTWHGNVRELEHTIERAVALERTDAIQPERLPEHITNYNPERIKAEFDLPDEGI
                     NLVTHLDNLEKTYVLEALKRTGGNQTRAAELLQMQVRSLRHLLDKHSIRTLSAQMRGS
                     GE"
BASE COUNT          404 a          444 c          480 g          484 t
ORIGIN      
        1 gggatagcga tggcggcgat aatgccgatg ataaccacga cgattaaaag ttcgataagc
       61 gagaagcctt tttggttcgt tttcatttta tttcacattc tccttggaag ttcttttggt
      121 cttaattttt tctaccttgt ctcaggcact gctctataag acaatctccg tgccaaaaga
      181 aaagatgccc ggaaaatgcg aaaaaccgtt gtattttcag tgtttacggg agcctgtcaa
      241 attgaccgaa cggtaaaatg acgcttgcgc gggctgttaa ataacagtat ttggcatttg
      301 gcgggtgacg aaaaatgtca gaaaacttaa ctgataaccg ataactggga aatgaaagct
      361 aaaaatggag agtttaaaat aaattgagaa ctgaaaggga aataattccg tcctccagtt
      421 ctcggctctc agttctcgat tgtcggttat tttactcgcc cgaaccgcgc atctgcgccg
      481 aaagcgtccg gatgctgtgt ttgtcgagca ggtgtcgcag cgagcgcacc tgcatttgca
      541 gaagctcggc ggcgcgcgtc tggtttccgc cggttctttt taaggcttcc agcacataag
      601 ttttttccag attatccaga tgcgtgacga gattgatgcc ttcgtcgggc aaatcgaatt
      661 cggctttgat gcgttcgggg ttgtaattgg tgatgtgttc cggcaggcgt tcgggctgaa
      721 tggcgtcggt tctttccagc gcgacggcgc gttcaatcgt gtgctcaagc tcgcggacgt
      781 tgccgtgcca ggtgtaattt tccagaatct gctcggcgcg ctcgctgatg gaaagatttc
      841 tgcccgtctg gtcgcagtat ttttttataa aatgactgac taaagatgaa atatcctcgc
      901 ggcgctcgcg cagcggcgga aggcttatcg ggatgaccga gatgcgataa aaaaggtctt
      961 cgcggaaact tccgtcgacg gtcatcgctt ttaaatcgcg gttggtcgcg gcgatgacgc
     1021 gcgcgtcaat cggcaattcg tcgtgcgcgc cgacggggcg cacgcggcgt tcctgcaaaa
     1081 ctctgagcag cttgacctgc atcgcgggcg acatctcgcc gatttcgtcc agaaaaatag
     1141 ttcctttgtg agcggcttca aaaagtcctt tgcgattggc gttggcgccg gtaaacgagc
     1201 ctttcatata accgaaaagc tcgctttcga gcagcgtttc ggtaaacgcg ccgcagttta
     1261 tcgagataaa aggcttttcg gcgcgcggcg acatatcgtg aatcgcgcgc gcgaccaatt
     1321 ctttaccggt tcccgattcg cccgtaatta aaatagtcga ttggacttcg gcgaccgttt
     1381 caatcatttg ataaaccgcc tgcattttat ccgaatgacc gatgatgttt ttgatgctgc
     1441 cgcgttcgcg ctgcgcgcgt ttaaaggcgc ggttttcgac gatcagagct tgtttgtcga
     1501 gcgttttttt gacgattaat tttaattctt cgacatcgaa aggcttttga ataaaatcgt
     1561 cggctccgag cttaaacgcc tcgcgcgcgg tttcgaccga ggcgaaagcc gtcatcatca
     1621 caacgccgac gtccggcaaa accccgcgca cggcgcgcag catctcgatg ccgtccatat
     1681 caggcatttt cacgtcggag ataatcacct cagccggttc ggtttgaagt ttttcgagcg
     1741 cctcgaaccc gttgttcgcc gtgcggtcgc tgtgcccttc gccttcgaaa acgaggctta
     1801 aaagctgcct gt
//
LOCUS       DASWNE010000019         4423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_671804, whole genome shotgun sequence.
ACCESSION   DASWNE010000019 DASWNE010000000
VERSION     DASWNE010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671804"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="VGB68_00440"
     CDS             complement(<1..647)
                     /locus_tag="VGB68_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047722.1"
                     /translation="MKNFVGKLFVGRSAKFNVLLVLCLFAFVGLGCFGSGKNKDAKPI
                     PAAFLGDWQGQDGSSLSIRADGTGDYRSGGKKVEGGTAEVSEADKTISITFFGIGETL
                     KIDSPPQDDTMKLNGITYKRKGGFSVADTNSSDSKSDSSNKKEKTISTTKVNAAKYEV
                     PEDEQMQEMTRTALMDFNEAVQDGDFASFHSTISKTWQKQTTPEKFNQVFSEFIS"
     gene            complement(756..1655)
                     /locus_tag="VGB68_00445"
     CDS             complement(756..1655)
                     /locus_tag="VGB68_00445"
                     /inference="COORDINATES: protein motif:HMM:NF025606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4344 domain-containing metallopeptidase"
                     /protein_id="HEY0047723.1"
                     /translation="MSFKPALKFLPRNLFNLLGTLGMFLLVAGCVLQSVKNENQNKPP
                     EPQVVPTKPATVIAAEKKDKQQDAGDFNVEHLGVKNSRYSELDRKVKEEKLLEKAADT
                     LNRSLILPYDISLRTKDCGEVNAIYDSADHSITICYELMEHFHKLFRSEGSSEREADK
                     KMFDAVRFAFLHELGHALIDAYKLPITGSEEDAADRCSSYICLEEMGEDGISAVLAAA
                     DAFHIEAKQSSPSGKDFSDEHLLQEQRFYNTLCMIYGSNTTKYSYFVSQKYLPESRAA
                     RCPNEYERMAQSWSELLQKWRKD"
     gene            complement(1911..3410)
                     /locus_tag="VGB68_00450"
     CDS             complement(1911..3410)
                     /locus_tag="VGB68_00450"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002083.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEY0047724.1"
                     /translation="MKSQAITGNYQEANVDAIAVAVFKDEKATSGFLKDLDKLTGGLI
                     ASVIKSEEFTGKEGETVLFYVAPKGKVKASRILLVGIGEKTDYKTPGVAILAGTAARH
                     LRKLNVKSLALVPRSEAEAREVAAQAMQGVVTSQFELDKYKTKDKNDKTIDTFAICVE
                     GANEKELKAGLERGEIIGESINFTRDLSNEPPNVLTPTEMAKRAQQMARETGLKCEIL
                     DEKQMQKLGMGSLLSVSYGSEQPAKLIVLRYEPKKSTGKKGELTALVGKGITFDTGGI
                     SIKPAEGMDAMKYDMTGGGTVIGTMRAIGLLKPSVPVLGIVAAVENMPDGKATRPSDI
                     VTAMNGKTVEILNTDAEGRLILADAVAYAEQQGATRIVDMATLTGAVIIALGDHNTAV
                     LGNDDELVNEIIECGKEVGENFWQLPIGKEYSRAIKSDIADIKNIGGGRKAGTIIGAV
                     FIQEFVDKAKWAHLDIAGTAWADDTKPHQSKGPTGVAIRTLLRMVEKSQ"
     gene            complement(3523..3984)
                     /gene="smpB"
                     /locus_tag="VGB68_00455"
     CDS             complement(3523..3984)
                     /gene="smpB"
                     /locus_tag="VGB68_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003564285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEY0047725.1"
                     /translation="MSSDGKDIVNNRSAFHEYHILDRYEAGVALQGTEVKSVMVGRIQ
                     LKESYVAVKDGEIWLFNAHISPYSHGNRENHEPLRTRKLLLHRREIEKLERETTVKGM
                     TLVVTRIYWKNGRIKFEIGVAKGKKLFDKRETIKERTVERETRQQLKENSR"
     gene            4103..4176
                     /locus_tag="VGB68_00460"
     tRNA            4103..4176
                     /locus_tag="VGB68_00460"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4135..4137,aa:Gln,seq:ctg)
BASE COUNT          920 a         1151 c         1062 g         1290 t
ORIGIN      
        1 ttcgatataa attcgctgaa aacctgattg aatttttcgg gcgtggtttg tttctgccag
       61 gttttcgaga ttgtcgaatg gaaactggca aaatcgccgt cctgcaccgc ttcgttaaaa
      121 tccatcagag ccgtgcgcgt catttcctgc atttgctcgt cttccggcac ttcgtatttc
      181 gccgcgttga ctttagtcgt cgaaatcgtc ttttctttct tgttcgagct gtcggacttg
      241 ctgtccgagg aatttgtatc tgcgacgcta aaaccgcctt tgcgtttata cgtgatgccg
      301 ttcagcttca tcgtatcgtc ctgcggcggc gaatctattt tcagcgtctc gccgatgccg
      361 aaaaacgtga tgctgattgt tttatcggct tcgctgactt ccgccgtgcc gccttcgact
      421 tttttgccgc ccgagcgata atcgcccgtg ccgtcggcgc gaatcgaaag cgaagagccg
      481 tcctgaccct gccagtcgcc gagaaatgcc gccggaatcg gtttcgcgtc cttgtttttg
      541 ccgctgccga aacaacccag accgacaaaa gcgaataaac ataaaacgag cagaacgtta
      601 aatttagcgc ttctgccgac aaagagtttg cccacaaagt ttttcatatt tctttccttt
      661 ttaaattagc tgtttgaatt tgtttaaatt agctgtttga atttgttgcc gaaaccgtac
      721 ctgttttcca ggacgtttcc gccgatgctt tttgctcaat ccttgcgcca tttttgcagc
      781 agctcgctcc agctttgcgc cattcgctcg tattcgttcg gacagcgcgc cgcgcgcgat
      841 tcgggcagat acttctggct gacgaaataa ctgtatttcg tcgtgttcga gccgtaaatc
      901 atacataaag tattataaaa acgctgttcc tgcaaaaggt gctcgtcaga aaaatctttt
      961 ccgctcgggc tgctctgttt cgcctcgatg tgaaaagcgt cggcggcggc aagcacggcg
     1021 ctgattccgt cctcgcccat ttcctccaga cagatatagc tcgaacagcg gtcggcggcg
     1081 tcttcttcgc tgccggtgat cggcaattta taagcgtcga tcaaagcgtg tccgagttcg
     1141 tgcagaaaag caaaccgcac ggcgtcgaac atttttttgt ccgcttcccg ctcgctgctg
     1201 ccttcactac ggaaaagctt gtggaaatgt tccatcagtt cgtagcaaat cgttatcgaa
     1261 tggtcggcgg aatcataaat cgcgttaacc tcgccgcagt ccttcgtgcg cagagaaata
     1321 tcgtacggca gaatcaggga gcgattaagc gtgtcagccg ctttttccag cagtttttct
     1381 tctttgacct tgcggtcgag ttcgctgtag cgcgaatttt ttacgcccag atgctcgacg
     1441 ttaaaatcgc ccgcgtcctg ctgcttatct tttttctcgg cggcaatcac cgtcgcgggt
     1501 tttgtcggga cgacttgcgg ctcgggcggt ttattctgat tttcgttttt cacggattgc
     1561 aaaacgcagc cggcgaccag caaaaacatc ccgagcgtgc cgagcaggtt aaaaagattt
     1621 cgcggcaaaa attttaaagc aggtttgaaa gacatatttc gcaaccgcag attgacggtt
     1681 ggattgtgct gattttgcga ttagatgtca acgtgaattg aactcaaaga cgcaaagaaa
     1741 acaaatttca ccaaagattc tcatagataa ccgcacacta aaacaggatg gacaggattt
     1801 acaggataaa aaatcaaaaa gcgacgtttt gaatttttct tatcctgctt atcctgtcca
     1861 tcctgtttta ataattctct gcgcctcttg tgtctctaag gtgaaaaaat ttactgcgat
     1921 ttttcgacca ttctcaaaag cgtgcgaatc gccacgccgg tcggaccttt tgactgatgc
     1981 ggtttcgtat cgtccgccca ggcggtgccg gcgatgtcca gatgcgccca tttcgcttta
     2041 tcgacgaatt cctgaataaa gaccgcgccg ataatcgtgc ccgctttgcg cccgccgccg
     2101 atgtttttga tgtcggcgat atcggatttg atggcgcggc tgtattcctt gccgattggc
     2161 aattgccaga aattttctcc gacttccttt ccgcactcga taatttcgtt gaccagttcg
     2221 tcgtcgttgc cgagaacggc ggtgttatgg tcgccgagag cgataatcac cgcgccggtc
     2281 aaagtcgcca tatcgacgat tcgcgtcgcg ccctgctgct cggcataggc gacggcgtcg
     2341 gcgagaatca ggcgaccttc ggcgtcggta tttaaaatct cgaccgtttt gccgttcatc
     2401 gccgtgacta tgtcagacgg gcgcgtggct tttccgtccg gcatattttc cactgccgcg
     2461 acgatgccga ggacgggaac gctcggtttc agaagaccga tggcgcgcat tgttccgatg
     2521 accgttccgc cgccggtcat atcgtatttc atcgcgtcca taccttcggc gggcttgatg
     2581 gaaatgccgc ccgtgtcaaa cgtgatgcct ttgccgacca gcgcggtcag ctcgcctttt
     2641 ttgccggtcg attttttcgg ctcgtatctt aaaacgatta atttagcggg ctgttccgaa
     2701 ccgtatgaga cgctcaaaag cgaacccatt ccgagcttct gcatttgctt ttcgtccaga
     2761 atttcgcatt tcaagccggt ttccctcgcc atctgctggg cgcgcttcgc catttcggtc
     2821 ggcgtcaaaa cgttcggcgg ctcgtttgat aaatcgcgcg taaagttgat cgattcgccg
     2881 atgatttcgc cgcgctccag accggcttta agctcttttt cgttcgcgcc ctcgacgcag
     2941 atagcgaaag tatcaatcgt tttgtcgttt ttatctttcg ttttgtattt gtcgagttca
     3001 aactggctgg tgacgacgcc ctgcatcgcc tgcgccgcga cttcgcgcgc ttcggcttcg
     3061 gagcgcggca cgagggcgag gcttttgacg ttcagtttgc gcaggtgacg cgcggcggtt
     3121 cccgccagaa tcgctacgcc cggcgttttg taatccgttt tttcaccgat tccgacgagc
     3181 aagatacggc tcgctttgac tttgcctttc ggcgcgacat aaaacagaac ggtttcgcct
     3241 tccttgccgg taaactcttc ggatttgata accgaggcta taagtccgcc ggtcagtttg
     3301 tccaaatctt tcagaaaacc gctcgttgct ttttcgtcct tgaaaaccgc gacggcgatc
     3361 gcatcgacgt ttgcctcttg ataattacct gtaattgcct gagatttcat tccgtatttt
     3421 tcctcgttcc tggttgatta atggttaatt gccgatagtt aattgattct ttttactgac
     3481 gattaaacat cttactgtaa tattgccgtg taaatctata acttatctcg aattttcttt
     3541 caactgctgg cgcgtttcgc gctcgactgt tctttccttg atagtctcgc gcttgtcgaa
     3601 aagctttttg cccttcgcca cgcctatttc aaacttgatg cgcccgtttt tccagtaaat
     3661 gcgcgtgacg accagagtca ttcccttgac ggtcgtttcc ctttcgagct tttcaatctc
     3721 gcgccggtgc aacaataatt tgcgcgtccg cagaggctcg tgattctccc ggttgccgtg
     3781 cgaatagggc gatatatgcg cgttgaaaag ccagatttcg ccgtctttga ccgccacgta
     3841 agattccttt agctggattc gcccgaccat cacgcttttg acttccgtgc cctgcagcgc
     3901 aacgcccgct tcgtaccggt ccagaatatg atattcgtga aaggcggagc gattattgac
     3961 aatgtccttt ccgtcagatg acataaaagg atattttgcc ttgcgctagt ttaacaagca
     4021 agtcggaaag tgtttaaatc atttgatgag cgcgttttca gttgacaagt gcgcgctcaa
     4081 tccataaatt attcttagct gctgggagat cgtctaatgg taggactgca gtctctggat
     4141 ctgcctatcg gggttcgaat ccctgtctcc cagccatcat tttaattcgt aaatcggggt
     4201 aatcaggctt tttaaacgag gcttgattac cctttgatta tttcgatgcg aagtattata
     4261 aatgatttat tcttatgaat gactggcacg aaactcaaat ccgcgttcgc tacgccgaga
     4321 ccgataaaat gggcatcgtt caccattcga attatccgat ctggcttgaa atcggtcgca
     4381 gcgaatattg ccgggcgcgc ggtttttctt atctggagat gga
//
LOCUS       DASWNE010000020         2041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_36208, whole genome shotgun sequence.
ACCESSION   DASWNE010000020 DASWNE010000000
VERSION     DASWNE010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36208"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VGB68_00465"
     CDS             complement(<1..380)
                     /locus_tag="VGB68_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047726.1"
                     /translation="MKIFTVFSLLLVGYLTIAAQPKPAGSIVAETGEPVLIDNRGAGL
                     SFALTEAEYKNIMKRLATERNYIQIKNKPRKLTAEARFGLNLVVNGKNIGWILNGNEE
                     RGYVFYADWNADGNLNNDAATRLK"
     gene            complement(392..1759)
                     /locus_tag="VGB68_00470"
     CDS             complement(392..1759)
                     /locus_tag="VGB68_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047727.1"
                     /translation="MKFKTCAIALSLIINWLALLTIAGAQNAINQSPDDLPNPYLLTR
                     DNIRRNNYITPIFELKAREKEYLASEQWKTSYLERIIQLESYVGNYDEAYAYEDLLYA
                     GFPTTEKIIEQYKKDIADLKSSPLADYKMLDAVAAIDSIAGGRQVVMINEEHRTPFHR
                     AFTLRLLEKLYARGFRYFAAETVEQSDTDLNKRGYPTQNTGYYTADPIYADLIRTALR
                     LGYKVVPYESADASCRSPENNPEFCSDKREREQARNLYERILKNDSQAKVFVHVGRGH
                     NSKAEISKEFNFMAYYFKEISKIEPFAIDQLRFSQRFNPALEQPLYRFLTRADLLKKP
                     SVFQSTNGSFYNQGMGYDMLIFHPRLRYENGRATFLQMDGIRRAQKINSEKLKIARRG
                     GIFTGAEPVLVQAFYARESDAAVPVDQIIVYPNRQIPVLLLPKGKFRFRSLERSGKLI
                     AEYQD"
BASE COUNT          466 a          512 c          486 g          577 t
ORIGIN      
        1 ttttttaatc tggtcgcggc atcgttgttt aaattcccgt ccgcgttcca gtcggcgtaa
       61 aacacataac cgcgctcctc gttgccgttg agaatccaac cgatattctt gccgtttacg
      121 accagattta atccgaaacg cgcctcggcg gtcaattttc gcggcttgtt ctttatctga
      181 atgtaattgc gctcggtcgc gagccgtttc attatatttt tatattcggc ttcggtcagc
      241 gcaaagctga gccccgcgcc gcggttatca atcaaaaccg gctcgccggt ttcggcaaca
      301 atcgaaccgg cgggtttggg ctgcgcggca atcgttaaat atccgacgag cagcagcgaa
      361 aaaaccgtaa aaattttcat aataatttcc tttaatcttg atattcggca atcaatttcc
      421 ccgacctttc cagagagcga aagcgaaatt ttcctttcgg cagcagcaga accggaattt
      481 gccggttcgg ataaacgatg atttggtcga ccgggacggc agcgtcgctt tcccgcgcgt
      541 aaaacgcctg caccagaacc ggctcggctc cggtaaaaat tccgccgcgc cgcgcgattt
      601 ttaatttttc cgaatttatc ttttgcgccc gccggattcc gtccatttgc agaaacgtcg
      661 cccgcccgtt ttcgtagcgc aggcgcgggt gaaaaatcaa catatcgtag cccatgccct
      721 gattataaaa actgccgttc gtcgattgga aaacgctcgg tttttttaaa aggtcggctc
      781 tggtcagaaa ccggtaaagc ggttgttcga gcgccgggtt gaaacgctgg ctgaaacgca
      841 actggtcgat ggcgaacggc tcgattttgc tgatttcctt aaagtaatac gccataaaat
      901 taaactcttt ggaaatctcg gctttggaat tgtgaccgcg cccgacgtgg acgaaaactt
      961 tcgcctgcga atcgtttttt aaaattcgct cgtaaagatt tcgcgcctgc tcgcgctcgc
     1021 gtttgtcgct gcaaaactcg ggattgtttt caggcgagcg gcagctcgca tccgcgcttt
     1081 cataaggcac gactttataa ccgagtctca aagccgtccg aatcaaatcg gcgtaaatcg
     1141 ggtctgccgt gtaatagccc gtgttttgcg tcgggtagcc gcgcttgttt aaatccgtat
     1201 cgctttgttc gacggtttcg gcggcgaaat agcgaaatcc gcgcgcgtaa agcttttcga
     1261 gaagcctgag cgtgaaagcg cgatgaaacg gcgtccggtg ttcctcgtta atcatcacca
     1321 cctgccgccc gccggcaatc gaatcgatcg ccgccactgc gtcgagcatt ttataatccg
     1381 cgagcggcga gctttttaaa tcggcgatgt cttttttata ctgctctatt attttttcgg
     1441 tcgtcggaaa accggcgtaa agcaaatctt cgtaagcgta agcttcatcg taattgccga
     1501 cataggattc cagttgaata atgcgttcca gatacgacgt tttccattgc tctgaggcga
     1561 gatattcttt ttcgcgggct ttcagctcga aaatcggcgt aatgtaattg ttgcgccgga
     1621 tattatctct ggtcagcaga tacggattcg gcaaatcgtc cggcgattga ttaatcgcgt
     1681 tttgcgcgcc ggcgattgtt aaaagcgcca gccaattgat aattaaagac aaggctatcg
     1741 cacaggtttt gaatttcata accttaataa tttctccgag tgcctgattt acactcttta
     1801 gattgggttg aatattttca ggaaaaccgc cggacaagta acgtttccgc attgccgcgg
     1861 gcgattctca gtctgcattt aaaccggcgt cggattttta cttaatcaat tcttccaaaa
     1921 actttatcgc ttccggagaa ttattgtccc ggagcgcata aatcgcttcg agccttaatt
     1981 tgtccgattt atcgtttctg gcgatttcca aaagttttct cgaagccggc gtttgtttta
     2041 t
//
LOCUS       DASWNE010000021         6530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_283558, whole genome shotgun sequence.
ACCESSION   DASWNE010000021 DASWNE010000000
VERSION     DASWNE010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6530
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283558"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VGB68_00475"
     CDS             complement(<1..236)
                     /locus_tag="VGB68_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047728.1"
                     /translation="MIAAGTVAVLSNFGGIFSPNENMNIALAEDIPVSRGISDPSAVS
                     DEQNSIEVYKTLAPGVAFITTTSTSENFLGQSEEG"
     gene            complement(475..834)
                     /locus_tag="VGB68_00480"
     CDS             complement(475..834)
                     /locus_tag="VGB68_00480"
                     /inference="COORDINATES: protein motif:HMM:NF039183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HEY0047729.1"
                     /translation="MSRPTILLFALNLFDAVLTIYWVRNGFASEGNQLMAGLLDMGNA
                     PFLIVKIAVGALAAVVLWRWSDLRIAKYGLTLALVVYVGLMGVHFVTGLSAFGYISEA
                     VINDFAVWSHKILAFFF"
     gene            1171..1893
                     /locus_tag="VGB68_00485"
     CDS             1171..1893
                     /locus_tag="VGB68_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047730.1"
                     /translation="MRRLFNLGYFTAVLGLVFAFGAIDATAQKRKPVLRKKPVVKRAA
                     VIRAPALYRIDTGTNIRVRMNGTLSSKTARVGDRFTTTVTEPVYSTNGVVVIPVGSTV
                     TGRVDTVTASRKGGKPGEISVSFVSVKLPNGATRAINGSLTDLNTKSAKSDNEGTASG
                     DKMKHRKVIFIGGGAGGGAVLGGAFGGGKGALIGGIIGGIAGAIAENQTKGEEAEVRS
                     GTEFGVYLNQSVSMAKWNEPIN"
     gene            2163..3206
                     /locus_tag="VGB68_00490"
     CDS             2163..3206
                     /locus_tag="VGB68_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047731.1"
                     /translation="MINKFFAFTLAMLFVLNTAAFADTKKPKARKAASQTNRLVALLP
                     ASDGVMFLDVQRLLNEALPQILSANPQKLTEINEHIDQIRAQSGLDVRQFEQIAVGVT
                     FKQTATSKTAVEPIVLAYGKYNSDALLTLAKIALKGKYREEKIGSKAVYIFAAKEILT
                     ENKAKFTGNSMLKIFDFLSRKMPSEIAVTSLNGNTLAIGSIARVRETVENKARVGADI
                     TMLLNRKPNSIMSFAAKTPVGLSQFFNLDDDMLGQSLSSIRQIYGAVNLSGENIVATV
                     GAKSLNAEQAKSLEETLGGMQALIRGFLSSARSEEKQVYARIVDSAKIARVGTEITID
                     LQVANADVGLLIK"
     gene            3300..4955
                     /gene="dacB"
                     /locus_tag="VGB68_00495"
     CDS             3300..4955
                     /gene="dacB"
                     /locus_tag="VGB68_00495"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEY0047732.1"
                     /translation="MTKTKHIRRRPLAAALTLTIACFGLIYGFSAASAQETQRERVIA
                     APTPTVTAKPTVSPTVSPATVPTATPRLAALQTIPELQAKIRSIILRPQLQRGQIGVK
                     IISLDTNKVIFEENAEKYFMPASNMKSFTVSAALEKLTPDFRFVTSVYAPASPDASGT
                     IRGDLIIYGRGDPSISTAFNDKDYFKGMNNLAEKIVASGVKRVEGALIGDESYFTGDA
                     LPASWEWDDLQWYYGAEVSALTVNDNALDLIVRPGSAIGAPAGVQLLPNVNGVLLVNN
                     TKTSAAGTRRDIGIRRRLGTNVIEVFGSMPLGAGAYTNYVSLPKPALVFVSMLRQLLE
                     QKGVVVTGQTRAVDAEFRKNAPLPANLVEVTKLESPPLALIAAKTMKPSQNLYTELIL
                     RAMGESIGDRANQRWTSADRGIAVVDKFLQEIGVAAGSVLMYDGSGLSRHDLITPSAV
                     VQLYSYMSRSRFAPVWRDSLTVAGVDGTLSNRFKGTRGENNVRGKTGTIDQVSSLSGY
                     MTTVAGERLAFSFITNNIPSGSLRTGTIDEIVVLLANFNGRTG"
     gene            5061..5939
                     /gene="dapF"
                     /locus_tag="VGB68_00500"
     CDS             5061..5939
                     /gene="dapF"
                     /locus_tag="VGB68_00500"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEY0047733.1"
                     /translation="MNPISFCKFHGFGNDYIVFEAEKLRDVASLADFAVGVCNRNTGV
                     GGDGIAVVEKLFDAAADYSCRIINPDGSEAGFSGNGTRCAVAYLHYKNLWREPNLRLQ
                     TRSGLKNYELLKRDGSAFQFLAELGKPKFDLHNAAHDDAAEIAGEKNLKMSGFSTFLL
                     PREGLALKFFYVNVGNPVACIFVEDFNEFDWRRAGRIMESNTEIFPERTNVVFIKTLD
                     MENIEIRIWERGAGETSSSGTCSIAAAVAHLRRENLRGTIQVRAPGGTTEAVWRADDE
                     MLITGRADLVFCGEIP"
     gene            complement(6019..>6530)
                     /locus_tag="VGB68_00505"
     CDS             complement(6019..>6530)
                     /locus_tag="VGB68_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEY0047734.1"
                     /translation="TGSTVRAEGATDETNWSVSYEILVPRATNLKLSTYNGGIHLSGV
                     DGSIEFEATNGGIHLTDLAGNVKGRTSNGGLHITLSGSSWRGSGLDVETTNGGIHLSI
                     PETYAARIETGTVNGGFKSDIAALNVEKTERTRRTRISADLNGGGAPVRVITTNGGVK
                     ITSSSNKGS"
BASE COUNT         1633 a         1658 c         1715 g         1524 t
ORIGIN      
        1 ccttcctcgc tctgccctaa gaaattttcg ctggttgagg tcgtggtaat aaaagctacg
       61 ccgggcgcga gagttttata aacctcaatg ctgttttgtt cgtcgctgac ggctgacggg
      121 tcggagattc ctcgcgacac gggaatgtct tccgccaggg caatgttcat attttcattc
      181 ggcgaaaaaa taccgccgaa attacttaaa acagctaccg ttcccgccgc aatcattgct
      241 gaagcgaatg ctattaataa aatctgacgc gctgataatt gatatattgc tttacttctc
      301 gacatagctt tttcctgtca cttcccgtag ttaaatttca accttttagt gccaaactaa
      361 gatgctcgac ttctgaaagt tcgttgcgta ttaaaaatac gaaaaacaaa aactttcggt
      421 ttcaataaat ataaaagccg aaagttttcg ccgcaagaat atttttacaa aaaattagaa
      481 gaaaaaggca agaattttat gtgaccagac ggcaaaatca ttgattacgg cttcggagat
      541 atagccgaag gcggaaagtc ccgtgacgaa atgaacgccc atcaatccga catagacgac
      601 cagagccagc gtcaagccgt atttggcgat gcgcaggtcg ctccaacgcc agagaaccac
      661 cgccgccagc gcgccgaccg caatcttgac gatgagaaac ggagcgttgc ccatatccag
      721 caagccagcc atcagttgat tgccttccga agcaaaaccg ttgcgaaccc agtaaatcgt
      781 caaaaccgcg tcaaaaagat tgagcgcaaa aagaagaatt gtaggacgag acatttccat
      841 aaatcgggca aaccccaaac aaaaaaatta taaaatttct cttgctttag aggcttgccc
      901 acagacagac gagactatcg gcaagcccct aacgggagaa atgcaatgtg tttatgctgt
      961 caatatactc ttaataaagc tcgttttcaa gaattttttt tcgaactcgc gaataagttt
     1021 aatttttcgc tttaacggtg ataatttaat tgttataagc agttagccga cacgtttaag
     1081 acaacggttt tttgcggcga agcgtcttac aagcgcttga ctctcccgag ttttaaagcg
     1141 gttttaataa atcaggaaac gaggtgcgag atgagaagat tgtttaattt gggttatttc
     1201 acggcggttt tgggattggt tttcgctttc ggcgcaatcg acgcaacggc gcaaaagcgc
     1261 aagccggttc tgcgaaaaaa accggtcgtt aagcgggcgg cggtaattcg cgcgccggct
     1321 ctatatcgaa tcgatacggg aacaaatatt cgcgtgcgaa tgaacgggac gctcagttca
     1381 aaaacggcgc gcgtcggcga tagatttacg acgaccgtca ccgagccggt ttattcgacc
     1441 aacggcgtag tggtgattcc ggtcggaagc acggtgacgg gtcgcgtcga tacggtcacg
     1501 gcttccagaa aaggcggcaa gcccggcgaa atcagcgtca gctttgtttc ggtcaaactg
     1561 ccgaacggcg cgacgcgcgc cattaacggt tctttgaccg atttaaatac gaaaagcgcc
     1621 aagagcgaca acgaaggcac ggcttccggc gataaaatga agcaccggaa agttatcttc
     1681 atcggcggcg gcgctggcgg cggcgctgtt ctgggcggcg cattcggcgg cggcaagggc
     1741 gctttgatcg gcggaattat cggcggcatc gccggcgcta tcgccgaaaa tcaaaccaaa
     1801 ggcgaagaag ccgaagttcg ctcgggtacg gagttcggcg tttatctgaa tcagtcggtt
     1861 tcgatggcga aatggaacga gccgattaac tagacgaaaa agaaagaatg aaaccacaga
     1921 tggacgcaga taaacgtaga taatttaacg ggatagacag gataatttaa aactgtaaac
     1981 gtcgaatttt cttttttatc ctgataatgc tgtctatcct gtttaaatct cgtgtttgtg
     2041 tgcatccgtg gtttcattct ttctttcttt tccgcggcta aaactcccag ccaactcttt
     2101 tcgcttcaat acacatcttt tattctgtaa tattggctta taatcatttc gagaggtttc
     2161 cgatgataaa caaatttttc gcattcacgc tggcgatgct tttcgtatta aacaccgccg
     2221 cttttgccga caccaaaaag ccgaaagcga ggaaagcggc gagccagacc aatcgcttgg
     2281 tcgcgcttct gcctgcctcg gacggcgtaa tgtttctgga cgttcagcgt cttttaaatg
     2341 aagcgctgcc gcagattctg tctgccaatc cgcagaaatt aaccgagatt aacgaacata
     2401 ttgaccaaat cagagctcag agcgggctcg acgtgcgcca gtttgaacaa atcgctgtcg
     2461 gcgtcacttt taaacagacg gcgacatcga aaaccgccgt cgagccgatc gtcttggcgt
     2521 acggaaaata caactccgac gcgcttttga cgctggcgaa aatcgctttg aaaggcaaat
     2581 accgcgaaga aaaaatcggc agtaaagccg tctatatttt cgcggcgaag gaaattttaa
     2641 ccgaaaacaa agcgaaattt acgggcaatt cgatgcttaa gattttcgat tttctctcca
     2701 ggaaaatgcc gtccgagatt gcggtcacga gccttaacgg caacacgctg gcaatcggct
     2761 caatcgcccg cgtgcgcgaa accgtcgaaa ataaagccag agtcggcgcg gacatcacaa
     2821 tgctgctcaa tcgcaaaccg aattcgataa tgagctttgc cgcgaagacg cccgtcgggc
     2881 tgagccagtt tttcaatctg gacgacgata tgctcggtca atctctgtct tcgatacgac
     2941 agatttacgg cgcggtcaac ctgagcggcg aaaacatcgt cgcgacggtc ggcgctaagt
     3001 cattgaatgc ggaacaggca aaatcgctcg aagaaacgct cggcggtatg caggcgctta
     3061 tcaggggatt tctcagcagc gcgcgaagcg aagagaaaca ggtttatgcg agaatcgtcg
     3121 atagcgcgaa aatcgcgcgc gtcggcacgg aaattacgat tgatttgcag gttgcgaatg
     3181 cggatgtcgg cttgctcatc aaataacgcg aaaacggtat gaaacgtcaa aagggaaatg
     3241 agtgaaacaa aactcgtttc cctttttgct ttccgctttc taatttataa ttttcatcga
     3301 tgacaaagac aaaacatatt agacgacgcc cgctcgcggc ggctttgacg ctgacaatcg
     3361 cctgtttcgg attgatttac ggtttttccg cagcgtcggc gcaggaaacg cagcgcgaaa
     3421 gagttatcgc cgccccgacg ccgaccgtta cggcgaagcc gacagtttcg ccgaccgtct
     3481 cgccggcgac cgtgccgacc gcgacgccgc ggcttgccgc gcttcagacg attcccgaat
     3541 tgcaggcgaa aattcgctcg attatattgc gcccgcagct ccagcgcggg cagatcggcg
     3601 tgaaaatcat ctcgctcgac acaaacaaag tcatttttga agaaaacgcc gaaaaatatt
     3661 ttatgcccgc ctcgaatatg aaaagcttca ccgtttcggc ggcgctcgaa aagctgacgc
     3721 cggattttcg tttcgtcacc agcgtctacg cgcccgcgtc gccggacgcg agcggcacga
     3781 ttcgcggcga tttgataatt tacgggcgcg gcgacccgag catttcgacg gcgtttaacg
     3841 acaaggatta ttttaaggga atgaataatc tggcggaaaa aatcgtcgct tccggcgtca
     3901 agcgcgtcga aggcgcgctc atcggcgacg aaagttattt taccggcgac gcgctgcccg
     3961 caagctggga atgggacgat ttgcagtggt attacggcgc ggaggtttcg gctttgaccg
     4021 tcaacgataa cgcgctcgat ttgattgtcc gtcccggctc ggcaatcggc gcgcccgccg
     4081 gcgttcagct tttaccgaat gtaaacggcg ttcttctggt caacaacacg aaaacgagcg
     4141 cggctggaac aagacgcgac atcggcattc gccgacggct cggaaccaac gtcatcgaag
     4201 ttttcggctc gatgccgctt ggtgcgggcg cgtatacaaa ctacgtttcg cttccgaaac
     4261 cggcgctggt tttcgtctcg atgctgcgtc aattgctgga gcaaaaaggc gtcgtcgtta
     4321 ccggacagac gcgagccgtc gacgccgaat tccgcaaaaa cgcgccgctt cccgcgaatc
     4381 tggtcgaagt gacgaagctc gaatcgccgc cgctcgccct gatagccgcg aaaacgatga
     4441 agccgagcca gaatctttac accgagctga ttttgcgcgc gatgggcgaa agcatcggcg
     4501 atagagcgaa ccagcgctgg acgagcgccg accggggcat cgccgtggtc gataaatttt
     4561 tgcaggaaat cggcgtcgcc gcgggcagcg ttttaatgta tgacggctcc ggcttgtcgc
     4621 gtcacgattt aattacgccg agcgccgtcg tgcagcttta ttcgtatatg agccgcagtc
     4681 gctttgcgcc ggtctggcgc gacagtttga ccgtcgccgg cgtcgacggc actttatcga
     4741 accgttttaa agggacgcgc ggcgaaaaca acgtgcgcgg aaaaacggga acgattgacc
     4801 aggtctccag cttgtcagga tatatgacga ccgtcgcggg tgagcgtctg gctttttcct
     4861 ttatcacgaa caatattccg agcggcagcc tgcgcacggg aacgattgac gaaatcgtcg
     4921 ttctgctggc aaattttaac ggaagaacag gataaaagaa acgcgaaacg ccgaatgcga
     4981 aatgcggaat gtgtggttcg tcattcctcg ttcggcattc cgcgtttttc tttctccgtg
     5041 ttctccgcgg ctgaaaattt atgaatccaa tcagcttctg caaatttcac ggtttcggca
     5101 atgactacat cgtttttgaa gccgaaaaat taagagatgt cgcttcgctg gcggattttg
     5161 cagtcggagt ctgcaaccgt aatacgggcg tcggcggcga cggcatcgcg gtggtcgaaa
     5221 aacttttcga tgcggcggct gattattcgt gccgcattat taacccggac ggcagcgaag
     5281 ccggattttc cggcaacggg acgcgctgcg ccgtcgctta tcttcattac aaaaatctct
     5341 ggcgcgagcc gaatctgcgc ctgcaaacca ggagcggatt aaaaaattac gaattgttaa
     5401 agcgcgacgg cagcgccttc caatttctcg ccgaactcgg caaaccgaaa ttcgatttgc
     5461 acaatgccgc gcacgacgac gcggctgaaa tcgccggaga aaagaatttg aaaatgtccg
     5521 gtttttctac atttttactg ccgcgcgaag gtctggctct caaattcttt tatgtgaatg
     5581 tcggcaatcc ggtcgcctgc atatttgtcg aagatttcaa cgaattcgat tggcggcgcg
     5641 ccggacgaat aatggaatcg aacaccgaaa tatttcccga aagaacaaat gtcgttttca
     5701 tcaaaacgct ggatatggaa aacatcgaaa tccgcatctg ggaacgcggc gcgggcgaaa
     5761 cttcttcgtc cggcacgtgt tcgattgcgg cggcggtcgc gcatctgcgg cgcgaaaacc
     5821 tgcgcggcac gattcaagtc cgcgcgcccg gcggcacgac cgaagccgtc tggcgcgcgg
     5881 acgacgaaat gctgattacg ggtcgggcgg atttggtttt ttgcggcgaa attccgtaaa
     5941 taaaatacaa ggagcgggaa attatttccc gctccttgta ttttataaaa caatctgaac
     6001 ttcaaaatct tttgaaattt agctgccttt gtttgacgaa gaagtgattt tcacgccgcc
     6061 gttggtggtt atcacgcgaa cgggcgcgcc gccgccgttt aagtcggcgc tgatgcgcgt
     6121 tctgcgcgtt cgctcggttt tttccacgtt cagcgcggcg atgtcgcttt tgaatccgcc
     6181 gttgaccgtt ccggtctcga tgcgcgccgc gtaggtttcc ggtatcgaca ggtgaatgcc
     6241 gccgttggtg gtttcgacgt ccagcccgct tcctctccag ctgctgcccg acaatgtaat
     6301 atgcagcccg ccgttgctcg ttctgccttt gacgtttccg gcaaggtcgg tcagatgaat
     6361 gccgccgttg gtcgcttcaa actcgattga gccgtcaacg ccgctcagat gaatgccgcc
     6421 gttgtatgtc gaaagcttca gattcgtcgc gcgcggaact aaaatttcgt aagaaaccga
     6481 ccagttcgtt tcgtccgtag cgccttcggc acgaacggtc gagccggttt
//
LOCUS       DASWNE010000022         1683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_388941, whole genome shotgun sequence.
ACCESSION   DASWNE010000022 DASWNE010000000
VERSION     DASWNE010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388941"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VGB68_00510"
     CDS             complement(<1..600)
                     /locus_tag="VGB68_00510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HEY0047735.1"
                     /translation="MDSQNFERSYKYYDLIMAAFVAVILCSNLIGVHKVSSVNLPFYG
                     EYIYGAGVLFFPISYLFGDILTEVYGYARSRKVIWAGFGALIFASLMSLVVVNLPTAK
                     TMSPEQQQAVNLIFGQTPRIVLASLTAFWLGEFVNSFVLAKMKLWTRGKNLWMRTIGS
                     TVLGEIADSLVFYPIAFYGMWSNEQLISVMIGNYFIKVLW"
     gene            complement(853..>1683)
                     /gene="purD"
                     /locus_tag="VGB68_00515"
     CDS             complement(853..>1683)
                     /gene="purD"
                     /locus_tag="VGB68_00515"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEY0047736.1"
                     /translation="ADGLAAGKGVVVAKDRNEAVQAIDALENTVGKEAVSKIVLEECL
                     VGREVSLILFADGKNFALMPPARDHKRIGENDTGANTGGMGVITDASLLSAEQTREIV
                     EKIIKPTLDGCRAEGFEFRGILFLGLMLTDDGAQVLEYNARFGDPETQAILIRLKTDF
                     IEICEAIDARTLDRIEIEWQNGNSACVVLASKGYPAKAQAGDVIRGLDEAGASENVFV
                     FHAGTAKNESGEYITAGGRVLGVTATGNGLNEALSRAYEAVEKISWNGMQFRRDIGKH
                     "
BASE COUNT          406 a          473 c          380 g          424 t
ORIGIN      
        1 ccataaaacc tttataaaat agtttccgat catcacgctg attagttgtt cgttcgacca
       61 cattccgtaa aacgcaatcg ggtaaaaaac gagcgaatcg gcaatctcgc ccagaacggt
      121 cgagccgatt gtccgcatcc agagattttt accgcgcgtc cataatttca ttttcgccag
      181 cacgaaggaa ttgacaaact cgccgagcca gaaagccgtc agcgaagcca gaacgatgcg
      241 cggcgtctgc ccgaaaatca gattgacggc ttgctgctgt tccggcgaca tcgttttcgc
      301 ggtcggaaga ttgacgacga ccagcgacat caaagaggcg aaaatcagcg ctccgaagcc
      361 cgcccaaatc acttttcggg agcgcgcgta accgtaaact tccgtcagaa tatcgccgaa
      421 caggtaactt atcgggaaaa acaaaacgcc cgcgccatag atatattcgc cgtaaaacgg
      481 cagattcacg ctcgaaacct tgtgcacgcc gataagattc gagcataaaa taacggcgac
      541 aaacgccgcc ataatcaaat cgtaatattt atagcttcgt tcaaaatttt gagaatccaa
      601 tgattagctc caaccgcgtt tctttaaaaa gcaggtttta tcaaaacata gtcttttttc
      661 gctgacaagt gttaatttgc cggcgattaa aaatatctcg aataactttc cgttcggaat
      721 tccgaaaatt tagtatttta tttcgccgcc gctttcgacg gcggaaattc tttcgtttcg
      781 cctaacaaat gaaaagcggc gagtaaaaag cgacaagaaa gaaacttctt attctttatc
      841 gcttttcact cgtcaatgtt taccgatgtc gcggcgaaac tgcattccgt tccagcttat
      901 tttttcgacc gcctcgtaag cgcggctcag cgcttcgttt aaaccgtttc cggtagccgt
      961 gacgcccaaa accctgccgc cagccgtaat gtattcgccg ctctcgtttt ttgcagttcc
     1021 cgcgtgaaag acaaagacgt tttcacttgc gcccgcttcg tccaagccgc gaatcacgtc
     1081 gcccgcctgc gccttcgccg gatagccttt tgacgccagc acgacgcacg ccgaattgcc
     1141 gttttgccat tcgatttcga tccggtcgag cgtccgggcg tcaatcgctt cgcagatttc
     1201 tataaaatct gttttcagac ggattaaaat cgcctgcgtt tccgggtcgc cgaaacgcgc
     1261 gttgtattcc agaacctgcg cgccgtcgtc ggtcagcatc aaaccgagaa ataaaattcc
     1321 gcgaaactcg aaaccttccg cccggcagcc gtccaaagtc ggtttgataa ttttttcgac
     1381 gatttcccgc gtttgctccg ccgataaaag gctcgcatcc gttatgacgc ccattccgcc
     1441 ggtgtttgcg ccggtgtcgt tttcgccgat tcgcttgtgg tcgcgcgcgg gcggcatcaa
     1501 cgcgaaattt ttcccgtcgg caaataaaat cagtgaaact tccctgccga ccaggcattc
     1561 ttcgagaacg attttgctga cggcttcttt gccgacggtg ttttccagcg cgtcgatggc
     1621 ttggacggct tcgtttctgt ctttcgccac gacgacgcct ttgcccgccg ctaaaccgtc
     1681 ggc
//
LOCUS       DASWNE010000023         5422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_283583, whole genome shotgun sequence.
ACCESSION   DASWNE010000023 DASWNE010000000
VERSION     DASWNE010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5422
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283583"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..743)
                     /locus_tag="VGB68_00520"
     CDS             complement(3..743)
                     /locus_tag="VGB68_00520"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0047737.1"
                     /translation="MELSGNTILITGGASGIGYAFARRFLAAGSEVIICGRRADKLRA
                     AKEIHPQLHTRVCDVSDEAERIALFDWAIGEFPRLNVLVNNAGIQRRVNLSESEDWQQ
                     TRSEIAINLDAPVHLSRLFIPHLLKRQRPAIINVTSGLSFVPLANVPVYCATKAALHS
                     FTMSLRQQLSETPVSVIEVAPPAVDTDLGGVGLHTFGVPLDEFADAVFRRLPQGETEI
                     AYGTAQQASRASRAELDEIFKFMNQAFR"
     gene            complement(815..1864)
                     /locus_tag="VGB68_00525"
     CDS             complement(815..1864)
                     /locus_tag="VGB68_00525"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEY0047738.1"
                     /translation="MLSARGYATRSPESKFEPFSFERRVVGAKDVLIEILFAGICHSD
                     IHQAKDEWGGSIYPMVPGHEIVGRVAQVGAEVTKFKEGDIAGVGCFVDSCRTCVNCAD
                     GFEQFCLVHCSQTYNSTEMDKKTPTYGGYSDKIVVDEAYTLKVSPDQPLANVAPLLCA
                     GITTYSPLRHFKVSAGQKVGVVGLGGLGHMGVKLAASMGAEVTVFSTSPSKEQDARKL
                     GAHNFVVTREPENMKPLAGQYDFILDTVSAQHDVNMYLNLLRLRGAMVLVGAPEKPLD
                     VHAFSIIVGNRTLAGSMIGGIAETQEMLDYCAANNITSDVEVISIRQIEEAYERTIKA
                     DVRYRFVIDMQTLNS"
     gene            2037..2762
                     /locus_tag="VGB68_00530"
     CDS             2037..2762
                     /locus_tag="VGB68_00530"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEY0047739.1"
                     /translation="MSRINIGTCGFGGKKIEYSQLLSAVEIQQTFYQPPQIKTLERWR
                     AEMPDRFEFALKAWQLITHEAKSPTFRRLKKVLSEKQREEAGYFKPTAIVREAWETTL
                     ACAEALRAKTILFQCPASFKPYAENIKNLEKFFKGINRGKMNFCWEPRGDWDDKIVRD
                     ICERLDLWHVVDPLKNRTVTPDRCYFRLHGMTGWRYKYEEGELAELATLLPANKTSYV
                     FFNNIYMTEDAVRFQEIIEENDL"
     gene            2855..3766
                     /locus_tag="VGB68_00535"
     CDS             2855..3766
                     /locus_tag="VGB68_00535"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY0047740.1"
                     /translation="MKPFLSIFLFLIFSLTAAAQTAVTLDGQVVCCADCWAEADRTKV
                     EYGTAEDLLKAKSCVEGGDPTLLAVREGDKFTLYRLEQGKFKLTEKNWLGYVGKKVSV
                     TGTRRKSKKADAFVVDALNVIAPSIAEREAQAALGKEVELKLKDLFGADVSLSQYKGR
                     IVVLNFWATFCIPCRKEMPDLAAIQNEYAALGLQVIGASTDESGNRPKVLQFIKETKV
                     NFPIWMGASAADMARFGLGEALPGTVVIDRSGKIVKVISGVVSVADLKKQIDSMLETA
                     ESGVTRTGTIAQTDAAEKPKKASSVPS"
     gene            3794..4318
                     /locus_tag="VGB68_00540"
     CDS             3794..4318
                     /locus_tag="VGB68_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047741.1"
                     /translation="MSGTGETNYKWTYGARFIPNVVARAKWRNTLTYQVNERLSVGVE
                     YNPLAGKVSPLANFVAVKETEKRPALILGTSSDRIGTPSGQSFYATLSKSLERETDLP
                     IAPYIGVAYGTYEKKARVIGGLNIYFPKGFSSTILYDGVSVHPLLNYAYKRHQFAFVL
                     VQGKKAGLSYSVSF"
     gene            4438..4818
                     /locus_tag="VGB68_00545"
     CDS             4438..4818
                     /locus_tag="VGB68_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047742.1"
                     /translation="MHRRSTKYLTFVILEEALRHYEAGIFTGNSPGYTDYVYDRIEKS
                     AKECAGFGGRCIDDVPGFPRRRFTPVMLSEIIDNIFTDLKNAEPSLARKRQLGRKHIE
                     INTTSFFNRIEAEIDRLYSLRKCV"
     gene            complement(4881..5132)
                     /locus_tag="VGB68_00550"
     CDS             complement(4881..5132)
                     /locus_tag="VGB68_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047743.1"
                     /translation="MVYINIPYMPENDTIDKDFAIQQIQTLDISDEAKWEAYKAIYNL
                     QPRGVAFESLAEALQLETVLKKLGVPYRQTETSEFTGTR"
BASE COUNT         1339 a         1418 c         1415 g         1250 t
ORIGIN      
        1 ttttatctaa aagcctgatt cataaactta aagatttcgt ccagctccgc gcgcgaggcg
       61 cggctcgcct gctgcgccgt gccgtatgcg atttccgttt cgccctgcgg caaacgccgg
      121 aaaaccgcgt cggcaaattc gtcgagcggc acgccgaaag tgtgcagacc gacgccgccc
      181 aaatccgtat cgacggcggg cggcgcgact tcgattacgg aaaccggcgt ttcggaaagc
      241 tgctgccgga gcgacattgt aaaagaatgc agcgccgctt tggtcgcgca gtaaaccggc
      301 acgtttgcca gcggcacgaa ggataaaccc gacgtgacgt tgataatcgc cggacgctgc
      361 cgtttgagca ggtgcgggat aaaaagtctg gaaagatgaa ccggcgcgtc gagattaatc
      421 gcaatctcgc ttcgcgtttg ctgccagtct tcgctttccg ataaatttac gcggcgctgg
      481 attccggcgt tattgaccag cacgttcagg cgcggaaatt cgccaatcgc ccaatcgaag
      541 agagcaatgc gctcggcttc gtccgaaacg tcgcagacgc gcgtgtgaag ctgcggatgg
      601 atttctttcg ccgcccgcaa tttgtccgcg cgccgcccgc aaataatcac ttcgcttccc
      661 gccgccagaa atctccgggc aaaagcgtaa ccgatgcccg acgcgccgcc ggtgattaag
      721 attgtgttgc cggataattc cataataact gatagtggat aactgataac tgatagttgt
      781 aatacattca ctatcagtta tcagttttca gttttcaact attcagagtt tgcatatcaa
      841 ttacgaaacg atagcggacg tcggctttaa tcgttcgttc ataagcttct tcaatctgcc
      901 ggatcgagat tacctcgacg tccgacgtga tattattcgc cgcgcagtag tcgagcattt
      961 cctgcgtctc cgcgatgccg ccaatcatcg aaccggcgag agtgcggtta cctacgataa
     1021 tggaaaaagc gtggacatcc aaaggttttt cgggcgcgcc gacgagcacc atcgcgcctc
     1081 gcaggcgcaa aaggttgaga tacatattca cgtcgtgctg cgcgctgacg gtgtcgagta
     1141 taaaatcata ctgaccggcg agcggcttca tattttccgg ctcgcgcgtg acgacgaaat
     1201 tatgcgcgcc cagtttgcgc gcgtcctgtt ctttcgacgg cgacgtgctg aaaaccgtca
     1261 cctccgcgcc catcgacgcg gcgagcttga cgcccatatg tcccaagccg cccaagccga
     1321 ccacgccgac cttttgtccc gcgctgacct taaaatgcct gagcggcgaa taagtcgtaa
     1381 tgcccgcgca tagcagcggc gcgacattgg cgagcggttg gtccggcgaa actttgagcg
     1441 tgtaagcttc gtcgaccacg attttgtcgg aatatccgcc gtaagtcggc gtttttttgt
     1501 ccatctcggt cgagttgtaa gtttgcgagc agtgaaccag acagaattgc tcgaaaccgt
     1561 ccgcgcagtt tacacacgtc cggcaggaat cgacgaaaca gccgacgccg gcgatgtcgc
     1621 cttctttaaa tttcgtgact tcggcgccga cctgcgcgac gcgcccgaca atttcgtgac
     1681 cgggcaccat cgggtaaatc gagccgcccc actcgtcttt tgcctggtga atatcggaat
     1741 gacaaattcc ggcgaaaaga atttctatga gaacgtcctt tgccccgacg acgcgccttt
     1801 caaaactgaa cggctcaaac ttcgattccg gcgaacgggt agcgtatccg cgtgctgata
     1861 acatttaatt tctccctttg attgtttgaa tataaatcta ccttaacaaa atcaattacg
     1921 aattacgaaa ttacgaatta caaatgaaga aaaatcttcg atgaaggtct cgaatatctt
     1981 taattcgcaa ttcgtaattt ataattcgtg attcataatt cataattgag ctggatatga
     2041 gcagaataaa catcggcacg tgcggcttcg gcggcaaaaa aatcgaatat tcgcagcttc
     2101 tctcagcggt cgagattcag cagacatttt atcagccgcc gcagataaaa acgctcgagc
     2161 gctggcgcgc ggaaatgccc gaccgattcg aattcgcgct gaaagcctgg cagttaatca
     2221 cgcacgaagc gaaaagcccg accttccggc gtctgaaaaa ggttttgagc gaaaagcagc
     2281 gcgaagaagc cggatatttc aagccgacgg cgattgtccg cgaagcctgg gagacgacgc
     2341 tcgcctgcgc cgaagcctta cgggcgaaaa cgattttgtt tcaatgcccg gcaagcttta
     2401 agccgtacgc ggaaaacatc aaaaatctgg aaaaattctt caaaggcatc aatcgcggca
     2461 aaatgaattt ctgctgggag ccgcgcggag actgggacga taaaatcgtc agagacatct
     2521 gcgagcggct ggatttatgg cacgtcgtcg accctttgaa aaaccgcacc gtgacgcccg
     2581 accgctgtta ttttcgcctg cacggaatga ccggctggcg ctacaaatac gaagaaggcg
     2641 agctggcgga actggcgacc ctgctgcccg caaataaaac ctcgtacgtt ttctttaaca
     2701 acatttatat gacggaagac gccgtcagat ttcaggaaat aatcgaagaa aacgatttgt
     2761 gatttccgtt atccgcggct gattttcgtg acatttcgcg gcaaatctct tattgaggtt
     2821 tgccggatag catttaaaaa gtcaaaataa tctggtgaaa ccgtttttat ccatattcct
     2881 gtttttaatt tttagcctga cggcggcggc gcagacggcg gtcacgctcg atggtcaggt
     2941 cgtctgctgc gccgattgct gggcggaagc cgaccgcacg aaagtcgaat acggcacggc
     3001 ggaagatttg ctcaaagcca aatcgtgtgt cgaaggcggc gacccgacgc tgcttgccgt
     3061 gcgcgaaggc gacaaattca cgctttaccg gctcgaacaa ggcaaattta aattgaccga
     3121 aaaaaactgg ctcggctacg tcggcaaaaa agtttccgtc accggcacgc gcaggaaatc
     3181 gaaaaaagcc gacgcgtttg tcgtcgatgc gctcaatgta atcgcgccga gcatcgccga
     3241 gcgcgaagcg caagccgcgc tcggaaaaga agtcgaatta aagctcaaag atttgttcgg
     3301 cgcggacgtc agtctcagtc aatacaaagg gcgcatcgtc gttttaaatt tctgggcgac
     3361 gttctgcatc ccgtgtcgaa aggagatgcc ggacctcgcc gcgattcaga acgaatacgc
     3421 cgcgctcggg cttcaggtca tcggcgcttc gaccgacgag agcggcaatc gcccgaaggt
     3481 tctgcaattt atcaaggaaa cgaaagtcaa tttcccgatt tggatgggcg cgagcgccgc
     3541 ggatatggct cgcttcggac tcggcgaagc cttgcccggc acggtcgtca tcgaccgcag
     3601 cggcaaaatc gtcaaggtca tttcgggcgt cgtcagcgtc gccgatttga aaaagcagat
     3661 tgattcgatg ctggaaacgg cggaaagcgg cgtgaccagg accggaacaa tcgcgcaaac
     3721 cgatgccgca gaaaaaccga aaaaggcgtc gagcgttccg tcgtgaccgc tctgaccggg
     3781 cgaaggtcgc gccgtgagcg ggacgggaga aacaaattat aaatggacat acggcgcgcg
     3841 gtttattcca aacgtcgtcg cccgcgccaa atggcgaaac actctgactt atcaggtcaa
     3901 cgagcggctt tccgtcggcg tcgaatacaa cccgctcgcc ggaaaagtct cgccgctcgc
     3961 caatttcgtc gccgtcaagg aaacggaaaa gcgtcccgcg ctgattctcg gcaccagctc
     4021 cgaccgcatc ggcacgccat cgggacaatc tttctacgcg actttaagca aaagcctgga
     4081 gcgcgaaacg gatttgccga ttgcgcctta tatcggcgtc gcctatggaa cttacgaaaa
     4141 aaaggcgcgc gtcatcggcg gcttaaacat ctatttcccg aaaggcttct cctcgaccat
     4201 tctctacgac ggcgtcagcg ttcacccgct tttaaattac gcctacaagc gacatcaatt
     4261 cgcttttgtg ctcgtgcagg gcaaaaaagc gggcttgagc tacagcgttt cgttctgacg
     4321 ctcgttgccg acctatctgc ttcacggctt caaaaaaaga ttgctgaccg cgcttgattc
     4381 ggcatatatt ctttacgatt caataaatcc ggcagaaacc gtcgatagcg caaagatttg
     4441 cacaggcgtt cgaccaaata tctgactttt gtaattcttg aagaagcttt gcgccattac
     4501 gaagcgggaa tatttacagg taattcaccg ggatatacgg attatgtcta cgataggata
     4561 gaaaaaagcg cgaaggaatg cgccggtttc ggcggcaggt gtatcgacga tgtgccgggg
     4621 tttccaagac gccggtttac gcctgtgatg ctgagcgaga ttattgacaa tatcttcacg
     4681 gatttaaaga acgcggagcc ttcgctcgcg cgtaaacgac aactcggtcg gaagcatatc
     4741 gaaataaata ccacctcatt tttcaatcgc atcgaagccg aaatagaccg attatacagc
     4801 ctgcggaaat gtgtataacg cgaagctgtc gtcgaatcgg caaaaccgca gcgagccgtt
     4861 ttataaataa caatttttct ttaccgcgtg ccggtaaatt cggaggtttc ggtctggcgg
     4921 taggggacgc cgagtttttt gagcacggtt tccagctgca gggcttcggc taggctttca
     4981 aaggcgacgc cgcgcggctg cagattatag atggctttgt aggcttccca tttggcttcg
     5041 tcgctgatgt ctaaagtctg gatttgctgg atggcgaaat ccttgtctat cgtatcgttt
     5101 tcgggcatgt aaggaatgtt tatgtaaacc ataatcttag tttagcacgg attcatttat
     5161 aaataacccg tgttccggca aaaccgcttt gcccgtccgg taattaaact caatttgtcg
     5221 tttcgcaaaa tattgcttta tcttcggctt cgcgcttaca atctacgcca agctattttt
     5281 tcaattcaaa attagaggag gattttgatg agattcccaa gagttctttt aagcctttgc
     5341 tttgtctttt gtttgtcgct ggcggcaagc gcggcgacct ttacggtgac gaaaaccgcc
     5401 gatacgaacg acggcgcgtg cg
//
LOCUS       DASWNE010000024         2868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_142239, whole genome shotgun sequence.
ACCESSION   DASWNE010000024 DASWNE010000000
VERSION     DASWNE010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142239"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VGB68_00555"
     CDS             <1..822
                     /locus_tag="VGB68_00555"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HEY0047744.1"
                     /translation="RRHRQISETDDLMEPRQINHDTTAKTILGQSLPTNQMIQQDLDD
                     ALDIIFAHPNVAPFVSVRLIRALVTSNPSPAYIARISAVFNDNGQGVHGDLKSVIRAI
                     LLDPEARDDNPAPQFGRLRSPMQHTIAFLRAMNANINEPLSFGYLYTGMGESILNAPT
                     VFGHYSPLYKMPGSNQLFAPEFQIYTPTETINRANFIYWMMNEGPVDLTPFTGIAANS
                     TELINAVDTRLLDGRMTPEMRASITKALLSSTDNRSRALTVLYLVAASGDFIIQR"
     gene            913..2214
                     /locus_tag="VGB68_00560"
     CDS             913..2214
                     /locus_tag="VGB68_00560"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEY0047745.1"
                     /translation="MSAGFGALAALSRFNLVKAAAPPAPLTDYKALVCVFLFGGNDGH
                     NMVVPLADSEYNAYQDIRGGLALAPNELLTINADSIPFGLHYGLPEMRNLYNQGKLAI
                     VANVGNLVKPTTRSDLSLLPNQLFSHADQTTQMQSGAPDGALGTGWGGRTADAMLASN
                     NGSSFPSSVSLSGAAAFSSGNLVQSASLEPGNDLRQPAMTFYPQPAADARALAQKEIV
                     TTASPSVFVNGANKIMNDAVNLNTILRNTVGNPPFTTQFPSTELGVRLREIARFIGLR
                     NTVGIGRQVFFCGMNGFDTHASQEWSHWNLLQQLSQALSAFHAATEEMGVAQQVTTFT
                     TSEFGRTLQPSGSGSDHGWGGHHFVLGGAVRGAAMYGSFPSMSLGAQHFLDSRGILIP
                     SLSLSQYGATLAKWFGVADAQLDSIFPTLPNFAARDLGFMF"
     gene            2679..>2868
                     /locus_tag="VGB68_00565"
     CDS             2679..>2868
                     /locus_tag="VGB68_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047746.1"
                     /translation="MDKENVINQENFDVLLNWLDRNREIAGQKYEKIRRRLIRIFLGR
                     GCFEAEELADETINRVTRK"
BASE COUNT          644 a          791 c          762 g          671 t
ORIGIN      
        1 cgtcggcatc ggcagatttc tgaaacagac gacctgatgg agccgcgcca aatcaatcac
       61 gacacgaccg cgaaaacgat tctcgggcaa agcttgccca ccaatcaaat gattcagcaa
      121 gacctcgacg acgcgctcga cattattttc gcacatccga acgtcgcccc atttgtctcg
      181 gtgcgcctga ttcgggcgct ggtaacgagc aatccgagcc cggcgtatat cgcgcgaatt
      241 tccgccgttt ttaacgacaa cgggcagggc gtgcacggcg atttgaaatc cgtcattcgc
      301 gcgattttgc tcgacccgga agcgcgcgac gataatcccg cgccgcaatt cggacgcctg
      361 cgaagcccga tgcagcacac catcgcgttt cttcgcgcga tgaatgcgaa catcaacgag
      421 ccgctttctt tcggatacct ctacacgggt atgggcgagt cgattttgaa cgcgccgacc
      481 gttttcgggc attattcgcc gctttacaaa atgccgggca gcaatcaatt gttcgccccg
      541 gaatttcaga tttacacgcc gaccgaaaca atcaaccgcg cgaattttat ctactggatg
      601 atgaacgaag gtcccgtcga tttaacgcct tttaccggca tcgcggcgaa ttcgactgag
      661 ttgattaacg cggtcgacac gcgtctgctg gatggaagga tgacgcccga aatgcgcgct
      721 tcgattacga aagcgctgct ctcttcgacc gataaccggt cgcgcgcttt gacggttctt
      781 tatctggttg ccgcgtcggg cgactttatt attcagcgtt agcgtccgaa gcgtctgccg
      841 cgagcgcgat tcggacaaaa aggagcaata ataatagaca aacataaatt ttcacgtcgc
      901 agctttttag gaatgagcgc cggtttcgga gcgctggcag ctctgagccg gtttaattta
      961 gtcaaagccg ccgcgccgcc ggcgccgttg accgattata aagcgctggt ctgcgtgttt
     1021 ctgttcggcg gcaatgacgg tcataatatg gtcgtcccgc tcgccgacag cgaatataac
     1081 gcttatcaag atattcgcgg cggcttggct ttagcgccga acgagctttt gaccattaac
     1141 gccgacagca ttcctttcgg cttacattac ggtttgccgg agatgcggaa tctttacaat
     1201 cagggaaagc tggcgattgt cgccaacgtc ggaaatctcg tcaagccgac gaccagaagc
     1261 gatttatcgc tgcttccgaa tcaattgttt tcgcacgccg accagacgac gcagatgcag
     1321 agcggcgcgc ccgacggcgc gctcggaacg ggctggggcg gtcgcaccgc cgacgcgatg
     1381 ctggcgagca acaacggttc gagcttcccg tcgtccgtgt ccctgagcgg cgcggcggcg
     1441 ttcagctcgg gcaatctcgt tcagtccgcc agccttgagc ccggcaatga tttaagacaa
     1501 ccggcgatga ctttttatcc gcagcccgcc gcggatgccc gcgcgctggc gcaaaaggaa
     1561 atcgtgacga ccgccagccc gtccgttttc gttaacggcg cgaacaaaat tatgaacgac
     1621 gccgttaatt taaatacgat actgcgaaac acggtcggca atccgccgtt tacgacgcag
     1681 tttccttcaa ccgagctcgg cgtacggctc agagaaatcg cgcgttttat cggtctgcga
     1741 aacacggtcg gcatcggtcg tcaggttttc ttttgcggaa tgaacggctt cgacacgcac
     1801 gccagccagg aatggagtca ctggaatttg ctgcaacagc tgagtcaggc tttatcggct
     1861 ttccacgcgg cgaccgagga aatgggcgtc gcccaacagg tgacgacttt tacgacctcg
     1921 gagttcggac gcaccttgca gccgagcggt tcgggttcag accacggctg gggcggtcat
     1981 catttcgttc tgggcggcgc ggtcaggggc gcggcgatgt atggaagctt tccgtcgatg
     2041 tcgctcggcg ctcagcattt tctcgattcg cgcgggattc tgattccgag cctctcgctt
     2101 tcgcaatacg gcgcgacgct ggctaaatgg ttcggcgtag ctgacgcgca gctcgattcc
     2161 attttcccga cgctgccgaa tttcgccgcg cgcgacctcg gatttatgtt ctaggcgcaa
     2221 agcggaaaac aatataaatt acctgttcag taaaagccgc acggtaacgc gccgtgcggc
     2281 tttcggcggc ttctgccgac agttaaattt tttcaatcgc cgaaaacatc cttttatatt
     2341 cgcggcttca aaaataatcc tgcgacgctt ttcccgaaat gcgctttccc ttttggtttg
     2401 cgcttttgat ttaagtttta tgccggcgaa aattttcaaa atccgcgccg cgtccggttc
     2461 gctcgtccgt tagcgcggcg aaatatcaaa aacctttcaa acaaacagtt ttaatcggcg
     2521 taatcgtttc aaaaagaatg aattttctgc tatattgcgg gcactcgaat cttcttaaac
     2581 ttaactgaag aatcgttttt aagaagattc cgcctggaat ttaatttctg cgaggttaaa
     2641 agtcagtcag taccgctttg cggttgattt acttcacgat ggataaggaa aatgtaatta
     2701 atcaggaaaa cttcgacgtg cttttgaact ggctcgaccg caaccgggaa attgccgggc
     2761 aaaaatacga aaaaattcgc cgccgcctga tacggatttt tctcgggcgc ggctgttttg
     2821 aagccgaaga actcgccgac gaaaccatca accgggtgac gcgcaagc
//
LOCUS       DASWNE010000025         1585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_530415, whole genome shotgun sequence.
ACCESSION   DASWNE010000025 DASWNE010000000
VERSION     DASWNE010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530415"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VGB68_00570"
     CDS             complement(<1..248)
                     /locus_tag="VGB68_00570"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047747.1"
                     /translation="MSILVFIEHKDCALNRLSLEAVAAAQSIGRDLGLKVTAVIPCDK
                     DCSLAQEIAAYDIEKVVVAKNEKLNIYTPDGYADAFEQV"
     gene            complement(328..1098)
                     /locus_tag="VGB68_00575"
     CDS             complement(328..1098)
                     /locus_tag="VGB68_00575"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEY0047748.1"
                     /translation="MKIVVLMKQVANKDAILRIGRDEKWIEEADLSYVTNESDGYALE
                     EALRLKERKGAGEVIVCSLGARRAQSTIKDALARGADRAIHIVVEESNQLSPFQIAKT
                     IADAIRDENADLVMTGLQSDDNSYGQTGVILAELLGIPHATIVIEVDASGADFRVKRE
                     LESGWYQWFTYPLPSLLTIQSGISQIRYASLKGIMAAKKKEIREVTPTIESFGSAQKI
                     RKVYLPLKTKQTQMLGDGDAKKGATELVEKLKTEVRVI"
     gene            complement(1271..>1585)
                     /locus_tag="VGB68_00580"
     CDS             complement(1271..>1585)
                     /locus_tag="VGB68_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ACP synthase"
                     /protein_id="HEY0047749.1"
                     /translation="NDKFETLAIKQVFGDYAYNLPVSSTKSMTGHTLGAAGGVEAALS
                     VLSIYNKTLLPTINYEFPDPDCDLDYIANEPRQKEVEYVLSNSFGFGGTNACLIFKRF
                     EE"
BASE COUNT          362 a          424 c          360 g          439 t
ORIGIN      
        1 acctgctcga aggcgtcggc gtagccgtcc ggcgtgtaaa tattcagctt ttcgtttttc
       61 gccacgacga ctttttcgat gtcgtaagcg gcgatttcct gagccaggct gcaatcctta
      121 tcgcacggaa taacggcggt cactttcaag cccaaatccc tgcctatgct ctgcgcggcg
      181 gcgaccgctt cgagcgaaag tctatttaaa gcgcaatctt tatgttcgat aaatactaaa
      241 atgctcatat taatttcgtc ctatgtcctt cgcccttcgt tcttcgtttt tttctaatga
      301 cgaagaacgc ggggcgaaag actaatttca aatgacgcga acctccgttt tcaatttttc
      361 gaccagctcg gtcgcgcctt ttttcgcgtc gccgtcgccg agcatctgcg tctgcttggt
      421 tttgagcggc agataaactt tccggatttt ctgcgccgag ccgaagcttt caatcgtcgg
      481 cgtaacttcc ctgatttctt ttttcttcgc cgccataatg ccttttaagc tggcgtagcg
      541 aatctgcgaa atgcccgatt gaatcgtcag aagcgacggc agaggatacg taaaccattg
      601 ataccagccg ctttccagct cacgcttgac gcgaaaatcc gcgcccgaag catcgacctc
      661 gataacgatg gtcgcgtgcg gaattcctaa aagctccgcc aggataacgc ccgtctgacc
      721 gtagctgtta tcgtcggact gcaagccggt catcaccaaa tcggcgtttt cgtcgcgaat
      781 cgcgtcggcg atggttttgg caatctgaaa cggcgaaagc tgattcgact cttcgacgac
      841 gatgtgaatc gcgcggtcag cgccgcgcgc cagcgcgtct ttgatggtcg attgcgcccg
      901 gcgcgcgccg agcgagcaga ctatgacttc gcccgcgcct ttcctttctt tcagacgcag
      961 cgcttcttcc agagcgtagc cgtccgattc gttcgtgacg tacgacaaat cggcttcttc
     1021 tatccatttt tcgtccctgc cgatccgcaa aatcgcgtcc ttgttggcaa cttgtttcat
     1081 taaaacgaca attttcatag aattattttt ccgtaagttt tttaatagta aatttcgcta
     1141 aatttttgtt aatttcaatg attctaaaac tttttcgccg aaacttccac tgaaaatgaa
     1201 tgaccattca ttcagctgat aaaattaatg cgcttaatcg ccgcagaagg caatcaaacg
     1261 catcaggaaa ctattcttcg aaccgcttga aaatcaagca ggcgttcgtt ccgccaaaac
     1321 cgaagctgtt tgacagaacg tattcgactt ctttctggcg cggctcgttt gctatgtaat
     1381 ccaaatcgca gtccgggtca ggaaactcgt aattgattgt cggcaaaaga gttttgttat
     1441 aaatcgagag caccgataaa gcagcttcga ctccgcccgc cgcgccgagc gtatgtccgg
     1501 tcatcgattt tgtcgagcta accggcaaat tatacgcata atcgccgaaa acctgtttaa
     1561 tggcgagcgt ttcaaacttg tcgtt
//
LOCUS       DASWNE010000026         2232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_566204, whole genome shotgun sequence.
ACCESSION   DASWNE010000026 DASWNE010000000
VERSION     DASWNE010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566204"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..550)
                     /locus_tag="VGB68_00585"
     CDS             complement(14..550)
                     /locus_tag="VGB68_00585"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY0047750.1"
                     /translation="METVKRTLTPLEGFSREIGFYLSSWEKSRAELREIVSDLSLEEL
                     AARILPGAHQIGGLILHLGEAESGWIHAIIAGKELDDEAKKFAHWYDTTETDFAEKDY
                     SARECIERIDKIGEMSRRILAQFDDEDLERLFGYRRDSGERVEVSLRWVLHHLIDHEA
                     NHKGQISMLKRLLRETQD"
     gene            730..>2232
                     /locus_tag="VGB68_00590"
     CDS             730..>2232
                     /locus_tag="VGB68_00590"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEY0047751.1"
                     /translation="MNKRFLTIAIIGCLLLLQTALPAAAQMSSVRYVIEDLGVLSAEP
                     SNYSLATGINSIGQATGITRGEFGEYRLFRWTNGTMENLGIISGTPDARTGATEINDF
                     GWITGTLKTPTEQIHAFLYNNSALLDLGGGGAEFPGGENITRGRGINNFGEVVGYQGE
                     NNPRAFYYDGTTMRNLNSLIPAGSGWILKFANDINDKRQIVGGGSNSNSEGGTRAYRY
                     TVGASSILAIFGGNSFSEAYSISNLGKTVGGSTNLPFSNGFAHADEFGTLQLGAPAPC
                     GGNSKAKDVNARGTIVGWYYPITGQSGCPQAFNERPVVWTPSGVAFELNNLIDPSLGW
                     TLTEANSINDRGQIVGTGTRVINGFVRTRAYRLTPIRPESNQVADFDGDGKSDISVFR
                     PADGDWHLLRSSTQDWTPVHFGAAGDKLAPGDYDGDGRTDLTVYRNGQWFILESGSGS
                     FRALQFGLPDDVPTPADFDADGRTDIAVFRPSNGYWYFTLSSDNLFRAVQF"
BASE COUNT          509 a          637 c          597 g          489 t
ORIGIN      
        1 tctctgcggc tatttaatct tgagtttcgc gcagcaagcg tttgagcatc gaaatttgtc
       61 ctttgtgatt ggcttcgtgg tcaatcagat gatgcaaaac ccagcgcagg ctgacctcga
      121 ctcgctcgcc gctgtcgcgc cggtaaccga acaatctttc gaggtcttcg tcgtcaaact
      181 gcgctaagat tcgccgcgac atctcgccga ttttgtcgat tctctcgatg cactcccgcg
      241 cgctgtagtc tttttcggca aaatccgttt cggtcgtgtc ataccagtga gcgaattttt
      301 tcgcttcgtc atccaattct tttccggcga ttatcgcgtg aatccagccg gattcagcct
      361 cgccgagatg cagaatcaag ccgccgattt gatgcgcgcc gggcaaaatt ctggctgcca
      421 gttcttcaag cgacaaatcg gaaacaattt cgcgcaattc ggcgcggctt ttctcccaac
      481 tgctcaaata aaaaccgatt tcccgcgaaa aaccttccag cggcgttaaa gttcgtttga
      541 ctgtttccat attcttattt tgccacagaa aagaatcagc cgcgaataag cgcgaataag
      601 acggctggca atcaaattca atgacatcaa acaaaatact gccatcttca caaacgagaa
      661 aattaggtgt aggatgaagc gtaactagcc ctgcccttca aaattcgagc cccacgggag
      721 aaaagattaa tgaacaagcg ctttttgacc atcgcgataa taggctgtct gctgctgctg
      781 caaacggctt tgcccgccgc tgcccagatg agcagcgttc ggtatgtcat cgaagacctc
      841 ggcgtgctga gcgcggaacc gagcaactac agtctcgcca ccggaatcaa ctcgatcgga
      901 caggcaaccg gcatcaccag aggcgaattc ggagaataca ggctgtttcg ctggacaaac
      961 ggcacaatgg aaaatctggg cataatcagc ggcacgcccg acgcccgcac gggcgcgacc
     1021 gaaatcaacg atttcggctg gattacgggc acgctcaaaa ctccgaccga gcagattcac
     1081 gcctttctgt ataacaattc ggcgttgctc gatctgggcg gaggcggcgc ggaatttccc
     1141 ggcggcgaga atattacgag aggcagaggc atcaacaatt tcggcgaggt cgtcggttat
     1201 cagggcgaga ataatccgcg agctttttat tacgacggca cgacgatgcg caatctcaac
     1261 tctttgattc cggcgggcag cggctggatt ctcaaattcg ccaatgacat caacgacaaa
     1321 cgtcagatag tcggcggcgg ctcgaattca aacagcgaag gcgggacgcg cgcctatcgc
     1381 tacaccgtcg gcgcgagcag catccttgcc atctttggtg gtaactcttt ctctgaggca
     1441 tattcaatca gcaatttagg aaagacggtc ggcggctcga caaatctccc gttttcgaac
     1501 ggctttgcac acgccgacga atttggaact ttacagttgg gcgcgcctgc cccgtgcggc
     1561 ggaaatagta aagccaagga cgttaacgcg cgcggcacga ttgtcggctg gtactaccct
     1621 ataacgggtc aaagcggctg cccgcaggct tttaatgaac gccccgtcgt atggacgccc
     1681 agcggcgttg cttttgaatt gaacaacctg atcgacccgt cgctcggctg gacgctgacg
     1741 gaagcgaact caatcaacga ccgagggcaa atcgtcggta ccgggacgcg cgtcatcaac
     1801 ggtttcgtgc gcacccgcgc ctatcgactg acgccgattc gcccggagag caatcaggtc
     1861 gcggacttcg acggcgacgg aaaatccgac atttccgtgt ttcgcccggc tgacggcgat
     1921 tggcatctgc tgcgttcctc gacgcaggac tggacgcccg tgcatttcgg cgcggcgggc
     1981 gataaactcg cgcccggcga ttacgacggc gacgggcgca ccgatttgac ggtttaccgc
     2041 aacggtcagt ggtttatcct ggaaagcgga agcggcagct tccgcgccct tcagttcggt
     2101 ttgccggacg atgtgccgac gcccgccgac ttcgacgccg acgggcgaac ggacatagcc
     2161 gtttttcgcc cgtcgaacgg ttactggtat ttcactctca gttccgacaa tctgttccgc
     2221 gccgtacagt tc
//
LOCUS       DASWNE010000027         7365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_671993, whole genome shotgun sequence.
ACCESSION   DASWNE010000027 DASWNE010000000
VERSION     DASWNE010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7365
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671993"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3794)
                     /locus_tag="VGB68_00595"
     CDS             complement(<1..3794)
                     /locus_tag="VGB68_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047752.1"
                     /translation="MPEEEVNQEEIEETPETTEEESGSPAAAAPPRRRFLTRRNFFIS
                     TAGLALIVLLLVLTGFFIYRYGYFDSYVKNQFITKMDQIGVDFTADTFRTTVAPMSLE
                     LGNATFVDKVTGEKLFHIDNAKLGLSVTDLYAWQLSRDFRVDSTEIDGAEIWVRFDEN
                     GNSNFSNLQFAEEERGYVNFNYTSVRVSLSNGLIHFGDLSRKVSADAKNVALQLEPEN
                     PEAPDEQKRYKFDFTSTKSIVVYDEKPIDPVDVRVRGITDRNGAEITELKLTSPLAES
                     TLTGNIEGWEQFRYSFRIDSTIDLTQTGTIFASGTALRGIGNFSGTVAGEGERYTIDG
                     EVTSDALAASNVRLKALQATAKVNVEDGIYEANGKAIAEMLTIEDFRIDFPQLIGNVR
                     GSGTDFKWFGELQAASAKTPQGTIASLYISDAVAEYKDEQLNATFGSVTARRFFSEEA
                     EIEFLRARNARLNYAGGRADVSLPNVSANNLKVEGAELRGVQAGNVKVRNQNGRTDIQ
                     ASSLQAQNLQTKDANLRNLSANGVNVTTNGTTRASVNQLRAAEVNAQGAKIGNLNASN
                     VDIEQSGNETRVNSDRLQVGSLTTDQAVIGSLNIAGVRLSIRGGRVEATSGDIDAGNV
                     NLKNQGNLENVRLVKPVFVLEPSGRYRASLDMSLGGGVLGSVRLGAARANVIATNDEI
                     ALNNLTASVMDGNVNGNAVIALNNRSRSRVDADFTALDLSKLLALQGGRVIPIAGQTT
                     GRANLTFPGTNFKAASGTLTADFVASAGTTERGLVPVNGRLELTATNGLFDIQQANLN
                     TEKSSLNATGRFDLEGSDSNLNLALNSTDASEIERLIRVLNLSPELQQQLDENEIQLA
                     GNATFNGTLTGNLTSPTIEGRAALDSVLIRGRTLGSLAANLFLNPEAIEFRDGRLQQP
                     DGGNITFTASIPQTGTNNTSVRATLTRVNTGNLLAALPVNLPEAFRDLQGETSGTVNL
                     SGLPNAASGELNIVSNNAVVAGQAFDNFEVNLTFQNSLVNINKFEGRSADGFLRAGGT
                     YDTNSTVFNLDLEGRGLQLARLATLFTSSKDIPAVTGTIDLKATATGRTADSTSYNIN
                     FTGTGQNVTINENAVGSISFTGNTVNQQLNANLTATFGNQQQVITGSVNFADPNLPIR
                     AETTFNNTELTPYIALFRPPDSVTITGTATGRVFLEGNLYATNAAGEKVFSTENLRGS
                     AEFSQFGLHLNETPFMATSPLVVRFNGSEVTVDNARFAGSGSNLVVNGTKSLNAKGAN
                     NLT"
     gene            complement(3923..5050)
                     /gene="ribB"
                     /locus_tag="VGB68_00600"
     CDS             complement(3923..5050)
                     /gene="ribB"
                     /locus_tag="VGB68_00600"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEY0047753.1"
                     /translation="MPIATIEEAAADIAAGKMIIIVDDEDRENEGDLVCAAEKVTPEI
                     INFMVTHARGLICLPLTEERCDELLLPMQTAENTSGFGTAFTVSIEAREGVTTGISAA
                     DRAHTILTAVNPNSKPSDLARPGHIFPLRAKNGGVLVRVGQTEASVDMARIAGCYPAG
                     VVCEIMNDDGSMARMPELEKFAEKHALKIISVAELVRYRIHKETLVRRVVETDLPTVY
                     GKFRAVVFENIVNGETHIAMVLGEVSQTTEPVPVRVQTENITFAMFGCELGEASTAVN
                     SSLQKIAEAGRGVILYLRQREHNLDLIHQLQTYALMQEKGVDFQAARKETGYGGNRDY
                     GIGAQILKDLGLTKIRLLTNHPPRVSALDAFDLEIIESVPL"
     gene            5185..6420
                     /locus_tag="VGB68_00605"
     CDS             5185..6420
                     /locus_tag="VGB68_00605"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0047754.1"
                     /translation="MSFELKLAWKYFRARRKSLARFTAIVAVAGIASGVAALIVAQAL
                     ARGFADEMRDKILANTAHITIFRTDGGGMSEIQEIESKIKQIENVGSTARTTYESALI
                     IGRKTDAYCTLRAKTDFGESTPSSAETFKSSPDENAAVKSPADSPSLSLAPPVIGIGA
                     ELAEKTGLSAGDETEIIFPDGGIAPRTSRVTVREIFRTGLYDYDSTWIYVSFADLARI
                     SGRPAFLPNVLGVSAKNIYATGETADEIRRILGGEYKVLDWQEANRPLFAALSLERKV
                     SLAIISLIIFIAVLNITTTLALLVHERRLDVAILRTCGARTRSLITIFLLEGLFLGIA
                     GILSGVFLGLALCFAGNRFRIISLPSDVYSLGYIPLVPRVSDVLLIAAVAFALSLTAT
                     VYPAFRAARIKPLENLRNQ"
     gene            6671..>7365
                     /locus_tag="VGB68_00610"
     CDS             6671..>7365
                     /locus_tag="VGB68_00610"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEY0047755.1"
                     /translation="MFERYTEKARRVIFFARYEALQYGSHTIAPEHVLLGLMREDKTM
                     AARFFPYRSNASVDTIRREVEMRIILRERIPQSVELQLSPETKRILAFANEESERLKM
                     RNIGTEHLLLGILREERSIASEILFEQGVRLPSVREEVARMSGYPQFFNQDGKKENSH
                     LQEFTRDLTADAAEGKLDPLIGREQEVERIIEILCRRTKNNPVLIGEAGVGKTAIIEG
                     LAQKIIDREVPTFL"
BASE COUNT         1577 a         2019 c         1971 g         1798 t
ORIGIN      
        1 gttaaattat tcgcgccttt ggcgttcagc gattttgttc cgttaacgac caggttcgag
       61 ccggagccgg cgaaacgcgc gttgtcaacc gtcacctcgc tgccgttaaa acgcacgacg
      121 agcgggctgg tcgccataaa cggcgtttcg ttcagatgca gcccgaactg gctgaactcc
      181 gccgagccgc gcagattttc ggtcgaaaaa actttttcgc ccgcggcgtt cgtcgcgtaa
      241 agattgcctt cgagaaaaac ccgccccgtc gccgttccgg taatcgtcac gctgtcgggc
      301 gggcggaaca gagcgatata cggcgtaagc tccgtgttgt taaaagtcgt ctcggcgcga
      361 atcggcagat tcgggtcggc gaaattgacg cttcccgtaa tgacctgctg ttgatttcca
      421 aacgtcgccg tcagattcgc gttcagttgc tgattgaccg tatttccggt aaacgaaatg
      481 cttccgaccg cgttttcgtt aatcgtcacg ttttgtcccg tgccggtgaa attaatgttg
      541 taggaagtcg aatcggcggt tctgcccgtc gccgtcgctt tcaaatcaat cgttccggtg
      601 accgccggaa tatccttgct tgacgtgaac agagttgcca gacgcgcaag ctgcaaccct
      661 ctgccttcga ggtcgagatt aaaaacggtc gagtttgtat cgtaagtacc gcccgcgcgc
      721 aggaatccgt cggcggagcg accttcaaat ttgttgatgt tgacgagcga attctgaaac
      781 gtcagattaa cctcgaaatt atcgaaagcc tgaccggcga cgaccgcgtt gtttgaaacg
      841 atattcagct cgccgcttgc cgcattcggc aaaccgctca ggttaaccgt gccggatgtc
      901 tcgccctgta gatcgcggaa agcttcgggc aaattaaccg gcagagccgc cagcagattt
      961 cccgtattga cgcgcgtcag agtcgcccgg acggaagtgt tgttcgttcc ggtctgcgga
     1021 atgctggcgg tgaaagtgat atttccgccg tcgggctgct gcagtcttcc gtcgcggaat
     1081 tcaattgctt ccggattaag aaaaaggttt gcggcgagcg agcccagggt tcgcccgcgg
     1141 attaaaaccg aatcgagcgc ggcgcgtcct tcaatcgtcg ggctcgtcag atttccggtc
     1201 agcgttccgt tgaaagttgc gtttccggca agttggattt cgttttcgtc caactgctgc
     1261 tgaagctccg gcgagaggtt taaaacgcga atcaaacgct cgatttcgct ggcgtcggtc
     1321 gaatttaagg cgagattcag atttgaatcg gagccttcca aatcaaagcg tccggtcgcg
     1381 ttcagagagc ttttttccgt gttcagattc gcctgttgaa tgtcgaaaag cccgttggtc
     1441 gcggtcagtt cgagcctgcc gttgaccggc acgagcccgc gttccgtcgt tcccgcactg
     1501 gcgacaaaat ccgccgtcag cgttccgctc gccgctttaa aattcgttcc cggaaatgtc
     1561 aggttcgccc tgccggtcgt ctgtccggca atcggaatga cgcgcccgcc ctgcaaagcc
     1621 agcagcttgg aaaggtcgag cgcggtaaaa tcggcgtcga cgcgcgagcg gctgcgattg
     1681 ttcagagcaa tcacggcgtt gccgttgaca ttgccgtcca tcacgctcgc cgtcaaatta
     1741 ttaagtgcaa tttcgtcatt tgtcgcgatg acgtttgccc gcgccgcgcc cagccggacg
     1801 ctgcccagaa cgccgccgcc gaggctcata tcgaggctcg cgcgataacg accggaaggc
     1861 tcaagcacaa aaaccggttt caccagccgg acgttttcca gatttccctg attttttaaa
     1921 ttgacgtttc ccgcgtcgat gtcgcccgaa gtcgcttcga ccctgccgcc gcgaatcgag
     1981 aggcgaacgc ccgcgatatt taagctgccg ataaccgcct ggtcggtcgt caggctgccg
     2041 acctgcaggc ggtcggaatt aacacgcgtt tcgtttccgc tttgttcgat atcgacattc
     2101 gacgcgttca gattcccgat tttcgcgccc tgcgcgttga cttcggcggc gcgcaattga
     2161 ttgacgctcg cgcgggtcgt gccgttggtc gtcacgttga cgccgttagc cgatagatta
     2221 cgcagattcg cgtccttggt ttgcagattc tgcgcctgga ggctgctcgc ctgaatatcg
     2281 gttctgccgt tttgattgcg aactttgacg tttccggctt gcacgccgcg cagctccgcg
     2341 ccttcgacct ttaagttgtt ggcgctgacg ttcggcaagc tgacgtcggc tctgccgccc
     2401 gcgtaattca accgggcatt tcgcgcccgc agaaattcga tttcggcttc ttcggaaaaa
     2461 aatcttcgcg ccgtcacgct gccgaaagtc gcgttcagct gctcgtcttt atactcggca
     2521 accgcgtcgg aaatataaag actggcaatc gtgccctgcg gcgttttcgc cgacgccgct
     2581 tgaagctcgc cgaaccattt aaaatccgtt ccgctgccgc gcacgttgcc gataagttgc
     2641 ggaaaatcga tgcgaaagtc ttcgatggtc agcatttcgg cgatggcttt gccgttcgcc
     2701 tcgtaaattc cgtcctcgac attgactttt gccgtcgcct gcagagcttt cagacgcacg
     2761 tttgaagcgg cgagcgcgtc gctcgtcacc tcgccgtcaa tcgtgtatct ttcgccttcg
     2821 cccgcgaccg tgccgctgaa attgccgatg ccgcgcagcg ccgtgccgga agcgaaaatc
     2881 gttccggtct gcgttaaatc aatcgtcgaa tcaatcctga agctgtagcg aaattgttcc
     2941 cagccctcga tattgccggt cagagtcgat tcggcaagcg gcgaagtcag ttttaactcg
     3001 gtgatttccg cgccgttgcg gtcggttatg ccgcgcacgc ggacatcgac cgggtcaatc
     3061 ggtttttcgt cgtaaacgac gattgatttg gtcgatgtaa aatcgaattt ataacgtttc
     3121 tgctcgtcgg gagcttccgg gttttccggt tcgagctgga gcgcgacgtt tttcgcgtcg
     3181 gcggaaactt tgcgcgagag gtcgccgaaa tgaatcaagc cgtttgagag cgaaactctg
     3241 accgaagtgt aattaaaatt gacgtagccg cgttcttctt cggcaaattg cagattcgag
     3301 aaattcgagt ttccgttttc gtcaaatctc acccagattt ccgcgccgtc aatctcggtc
     3361 gaatcgacgc gaaaatcacg gctcagctgc caggcgtaaa ggtctgtcac gcttaatccc
     3421 agtttcgcat tgtcaatatg aaaaagtttt tcgcccgtga ccttatcgac gaaagtcgcg
     3481 tttcccagtt ccagagacat cggcgcgacg gtcgtccgaa aagtgtcggc ggtaaaatcg
     3541 acgccgattt ggtccatctt ggtgatgaac tggtttttga cgtaactatc gaaatagccg
     3601 tagcgatata tgaaaaatcc cgtcagaacc agaagcaaaa cgataagcgc caagcccgcc
     3661 gtcgagataa agaaattacg gcgcgtcagg aaccggcggc gcggcggcgc ggcggctgcc
     3721 ggcgaaccgc tttcttcttc cgtcgtttcc ggcgtttctt cgatttcttc ctggtttact
     3781 tcttcttccg gcataaaact tcaatgcaat aatgagccgc tgatttaata aaattgtcta
     3841 agatttttta ggattttatc ttttaatcgt aataaatctt aaaagtgcgc gttctatatt
     3901 tcctttttgc ctttcccgaa aatcaaagcg gcacgctttc gataatttcc aaatcaaagg
     3961 cgtcgagcgc cgagacgcgc ggcggatgat tggtcaaaag gcgaattttc gtcaagccca
     4021 aatctttcag aatctgcgct ccgatgccgt aatcgcggtt tccgccgtag ccggtctctt
     4081 tgcgcgcggc ttgaaaatcc acaccttttt cctgcatcaa ggcgtaagtc tgcaattgat
     4141 gaatcaaatc caaattgtgt tcgcgctggc gcaggtaaag aattacgcct cgtcccgctt
     4201 cggcgatttt ttgcagggaa gagtttaccg ccgtcgaagc ctcgcccagt tcgcagccga
     4261 acatagcgaa ggtgatattc tccgtttgga cgcgcaccgg cacgggttcg gttgtctgcg
     4321 aaacttcgcc gagaaccatc gcgatatgcg tttcgccgtt gacgatgttt tcaaagacga
     4381 cggcgcgaaa ttttccgtat acggtcggca aatcggtttc gacgacgcgg cgcacgagcg
     4441 tttccttgtg aatgcggtaa cgcaccagct cggcgaccga gataattttc aacgcgtgct
     4501 tttcggcaaa tttttccagc tccggcatcc gcgccatcga gccgtcgtcg ttcataatct
     4561 cgcagaccac cccagccgga taacagcccg cgatgcgcgc catatcgacg ctcgcttcgg
     4621 tctgaccgac gcgcaccagc acgccgccgt ttttcgcccg caacggaaaa atatgtccgg
     4681 gacgcgccaa atccgaaggc ttcgagttcg gattcacggc ggtcaaaatc gtgtgcgcgc
     4741 ggtcggctgc cgaaatgccg gtcgtgacgc cctcgcgcgc ttcgatggaa acggtaaaag
     4801 ccgtgccgaa cccggaagtg ttttccgccg tctgcatcgg cagaagcaat tcgtcgcagc
     4861 gctcttccgt cagcggcaga caaatcagcc cgcgagcgtg cgtcaccata aaattaatga
     4921 tttccggcgt taccttttcg gcggcgcaga ccaggtcgcc ctcgttttcc cggtcttcgt
     4981 cgtcaacgat gattatcatt ttaccagccg caatatcggc ggcggcttct tcaatagtcg
     5041 caattggcat cgatagattt tcgattttag atttcggatt tccaaattgt tcggcaaatc
     5101 gatttgctca aaccagaagt ttaccaaaaa gaaagcattt ggcgaacttt tattttgccg
     5161 atttccgaat tacgattgac ggcggtgagt ttcgagctaa aactggcgtg gaaatatttt
     5221 cgcgcgcggc gcaaatcgct ggcgcggttt acggcaatcg tcgccgtcgc aggcatcgca
     5281 tcgggcgtcg ccgcattgat tgtcgcgcag gcgctcgccc gcggatttgc cgacgagatg
     5341 cgcgataaaa ttctggcaaa cacggcgcac atcacgattt tccgcacgga tggcggcgga
     5401 atgtcggaaa ttcaggaaat cgagtccaaa atcaagcaaa tcgaaaatgt cggctcgacc
     5461 gcgcggacga cctacgaaag cgcgctgata atcggcagga aaacggatgc ctactgcaca
     5521 ctgcgcgcca aaacggattt cggcgaatcg acgccgtcga gcgctgaaac atttaaatcg
     5581 agtccggatg aaaatgcggc ggtaaaatct ccggcagact cgccctcttt aagcctcgca
     5641 ccgccggtaa tcggcatcgg cgcggaatta gccgagaaaa ccggcttgtc agccggcgac
     5701 gagaccgaga ttatttttcc ggacggcggc atcgcgccca gaacttcgcg cgtcacggtc
     5761 agggaaattt ttcgcaccgg actttatgat tatgattcga cctggattta cgtttcgttc
     5821 gcggatttag ccagaatttc gggtcgcccc gcatttttgc cgaacgttct cggcgtttcc
     5881 gccaaaaaca tttacgcgac cggcgagacg gcggacgaaa ttcgccggat attaggcggc
     5941 gaatataaag ttttggactg gcaggaagcg aatcgaccgc ttttcgccgc tttgagtctg
     6001 gaaagaaaag tttcgctggc gattatctcg ctgataattt ttatcgccgt tttgaacatc
     6061 acgacgacgc tcgctttgct ggttcacgaa cgccgcctgg acgtggcgat tttgcggacg
     6121 tgcggcgcgc ggacgcggag cttaatcacg atttttctgc tcgaaggctt gtttctcgga
     6181 atcgccggaa tcctctccgg cgtgtttctg ggcttggcgc tttgctttgc cggaaatcgt
     6241 ttccggatta tcagcctgcc gtccgacgtt tattcgctcg gctacattcc gcttgtgccc
     6301 cgggtttcgg acgttctgct gattgccgcc gtcgctttcg cgctgagcct gacggcgacc
     6361 gtttatccgg ctttccgggc ggcgcgaatc aagcctctgg aaaatctgcg caaccaataa
     6421 cgaaattttc actactaaca gacaggtaaa gaaaacagga aaccggaaat gataaacgca
     6481 aaaggtgcaa ttcgcctttc cgcattcggc accgagcgtt tctttttcat ttcttctgga
     6541 ttttcgctta ttttatatat ttactatgtc aagcacggaa aactttccaa atctgaaaaa
     6601 aacttggcat ttatcggcag acttcattta taatcgtttt acacggaaaa gggtctgaag
     6661 ctgaattttt atgtttgaac gttacacgga aaaagcacgt cgggttatct tttttgcacg
     6721 ctacgaagca cttcaatacg gttcacatac aattgccccg gagcacgtcc ttttgggact
     6781 gatgcgcgag gacaagacga tggcggcgcg tttttttccg tatcggtcaa acgcgtcggt
     6841 cgatacgatt cgccgcgaag tcgagatgcg catcattttg cgcgagcgca ttccgcaatc
     6901 ggtcgaattg cagctttcgc ccgaaaccaa gcggattctc gcctttgcca atgaggaaag
     6961 cgagcgattg aaaatgcgca atatcggcac cgagcatcta ctgctcggaa ttttgcgcga
     7021 agaacgctca atcgcgtccg agattctttt cgagcagggc gtccgcctgc cgtccgtgcg
     7081 cgaagaagtg gcgcgaatga gcggctaccc gcagttcttt aaccaggacg gaaaaaaaga
     7141 aaactcgcat cttcaggaat ttacgcggga tttgaccgcc gatgccgccg aaggcaagct
     7201 cgacccgctg atcgggcgcg agcaggaagt cgagcgaata atcgagattt tgtgtcgccg
     7261 gacgaaaaac aatccggttc taatcggcga ggcgggcgtc ggcaaaaccg ctattataga
     7321 aggcttggcg caaaaaatta tcgaccgcga agttccgacc tttct
//
LOCUS       DASWNE010000028         2208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_283842, whole genome shotgun sequence.
ACCESSION   DASWNE010000028 DASWNE010000000
VERSION     DASWNE010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283842"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="VGB68_00615"
     CDS             <1..716
                     /locus_tag="VGB68_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047756.1"
                     /translation="GFAVAFIAFGIGLWKFMPSESVDGNEHLAKKESNNIRAISGQPD
                     ESNKGESKDLPAKDEIDKPVASAKAFKPVKESGSLKRKLNDGTTNKLVAAGTPETDRK
                     IISGGDGNYSPVKPSPGKEQLRQLQLSALEKATTQQIEKIELLLRSFRNARVNEGGDF
                     YDVGYEKQQARKLLERNVRLRQMAENYGTLYTEEILDRVEPLLLDIANLENNPLPEKV
                     LDIKARVKNQNIIASLQIY"
     gene            766..>2208
                     /locus_tag="VGB68_00620"
     CDS             766..>2208
                     /locus_tag="VGB68_00620"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0047757.1"
                     /translation="MNIESRLFKQFNKSVFKSGTTLLFVAALCIVLLAERKFAFESNP
                     SIPAAQNSGQDKDFDKIFREGRDLIDKEEWAKAAEKFNALASRFPDNKSTDAALYWLA
                     FCYKKQKQIKETDRALDRLLKDFPASSWADDARVMKMEIAVPLGRIIVSNNGIETTAP
                     AMGVYTRTLPGSQSKPASTIISTSPETLETFKTFGEMNAGSPQTPLDREDEIKIAAFQ
                     SLLSADAKKGIEAMGGILKSDSRASETLKLEVLRVVRRPRIANNYSSASPLGMTTTIN
                     SVNRELIPLLRESLVKSFQNEANIKIRKEIIYTLTSLKDEQSVKYLVELYFSENDREV
                     KKAIINGFGSWWGINGFSVAKISENNSVQNPNLIIAGGGQIQKIEFTKLLEIVRREKD
                     IELKSLALSNLQRSAGWETNGQSIEVLSGIYDAETNEDFKKSIIQSLGKIKQTPASRK
                     LLDIAKNDKSDKLRLEAIYALRGNNSPEAIK"
BASE COUNT          693 a          499 c          537 g          479 t
ORIGIN      
        1 tgggatttgc ggtcgcgttc atcgccttcg gaatcggttt atggaagttt atgccgagcg
       61 agagtgtcga cggcaatgaa catcttgcta aaaaagaatc gaataacatt cgagccatat
      121 ccgggcaacc ggacgaaagt aataaaggag aatcgaaaga tttaccggca aaagacgaga
      181 ttgataaacc ggtcgcttct gcgaaagcct ttaagcccgt taaggaaagc ggttctctga
      241 aacgaaaatt gaatgacggg acgacaaaca aactcgtcgc cgccggaaca ccggaaacag
      301 accggaagat aatttccggc ggcgatggaa attattcgcc cgttaagccg tcgcccggca
      361 aagaacaatt acgacaatta cagctaagcg ctttggaaaa agcgacgacg cagcaaattg
      421 aaaaaatcga attgctgctg cgctcgttca gaaacgctcg cgtcaacgaa ggcggcgact
      481 tttacgatgt cggttatgaa aaacagcagg ctcgcaaact gctggaaaga aacgtccggc
      541 tgcggcagat ggctgaaaac tacgggactt tatacaccga ggaaattctc gatagggtcg
      601 agcctttgct gctggacatc gccaatctgg aaaacaatcc tttgccggaa aaagttctgg
      661 acattaaagc gcgcgtcaaa aatcaaaata ttattgccag cttacagata tattgacttt
      721 gagccggaca aaaacaaaaa aacgagaata cacagaagag aaattatgaa tatcgaaagc
      781 cgccttttta aacaatttaa taaatcagta ttcaaatcag gcacgaccct gctgtttgtc
      841 gccgctcttt gcatcgtttt actggcggag aggaaatttg ctttcgagtc gaatccttcc
      901 attccggcgg cgcaaaacag cgggcaggat aaagatttcg ataaaatttt tcgtgaaggc
      961 agagacctga tcgataagga agaatgggca aaagccgccg aaaaatttaa tgcgcttgcg
     1021 agccggtttc ctgataataa atctaccgac gcggcgcttt actggctggc tttctgctat
     1081 aaaaagcaaa agcagattaa ggaaaccgac agggcgctcg accgcctgct gaaagatttt
     1141 cccgcttcgt cctgggctga cgacgccaga gttatgaaga tggaaatcgc cgttccgctc
     1201 ggaagaataa tcgtctcgaa caacggcatc gaaactaccg cgccggcgat gggcgtttat
     1261 acgagaacct tgccgggcag ccaatctaaa ccggcatcga ccattatttc gacctcgccc
     1321 gaaactttgg aaacttttaa gaccttcggc gagatgaatg ccggcagccc gcaaactccg
     1381 ctcgaccgcg aagatgaaat caaaatcgcg gcatttcaaa gtctgctttc cgccgacgcg
     1441 aaaaaaggaa tcgaagcgat gggcggaatt ttaaagtccg attccagggc gagcgagact
     1501 ttaaaactcg aagtcctgcg cgtcgtgcgc cgaccgcgca ttgcgaataa ttattcttcc
     1561 gcgtcgcctc tgggaatgac gacgacgata aattccgtca accgggagct tattcctctg
     1621 ctcagagaat ctctggtcaa aagctttcaa aatgaagcga atattaaaat ccgcaaagag
     1681 ataatttaca cgctgaccag cctgaaagac gagcagtccg tcaaatatct ggtcgagctc
     1741 tacttttcgg aaaacgaccg tgaagtaaaa aaagcgatta tcaacggttt cggttcgtgg
     1801 tggggaatca atggattttc cgtggcaaag atttcggaaa ataattcggt tcaaaatccg
     1861 aaccttatta ttgccggcgg cggtcaaatt caaaaaatcg agttcacgaa attgctggaa
     1921 atcgttagaa gggaaaagga catcgagctg aaaagcttag ccttaagcaa tctgcagcgt
     1981 tccgccggct gggaaacgaa cgggcaaagc atcgaggttc tgtccggaat ttacgatgcg
     2041 gaaacgaatg aagatttcaa aaaatcaatt attcaaagtc tcggaaaaat aaaacaaaca
     2101 ccggcttcga gaaaactttt ggatatcgcc aaaaacgata aatcggacaa attaaggctt
     2161 gaagctattt acgcgctccg gggcaataat tctccggaag cgataaag
//
LOCUS       DASWNE010000029         2695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_672169, whole genome shotgun sequence.
ACCESSION   DASWNE010000029 DASWNE010000000
VERSION     DASWNE010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672169"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..2626)
                     /gene="priA"
                     /locus_tag="VGB68_00625"
     CDS             complement(134..2626)
                     /gene="priA"
                     /locus_tag="VGB68_00625"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEY0047758.1"
                     /translation="MIPSLTKDSPAPRFAEVALPLPLRQTFTYSLPLALRETVKLGAR
                     LLVPFGNRQLTGYVVALHQNLSEEIEFEADAVKDVLELIDEEPLVSAEILRLTQWTAD
                     YYSASWGEILKASLPAGVNAAIEQIVQITSAGRDELIKLSGAKLNTSKAQILQMLAGN
                     AEVSQREIQKKFGASTTQRAVRELLKLNWISTFQRTLTTKAKPKRRKSVRLLPTEMHK
                     QAAKALTEAQAKVVETLINENGEIGFTELLEKAQISASAVNSLEKRGIVQVFVREVFR
                     DPLAHAKLPEILDLILTDWQTDAFREIEKAILSNDYHAFLLHGVTGSGKTEIYIRAMK
                     EALDTGKSSLMLVPEIALTPVFSRRLRAVFGDEVAILHSSLSTGERFDEWRRIRNGQA
                     RIAIGTRSAIFAPLKNIGLIIVDEEHDGSYRQHESPFYHARDVAVVRASFARATIVLG
                     SATPALESFHNAKSGKYVYLHLPNRIGDRPLAQAELVDMREVFRKEGRDAIFSPPLLN
                     AIEETFAKGEQSIILLNRRGFSQFVLCRSCGETIRCQNCDITLTYHKRENVLLCHYCG
                     FRAKAPRKCPFCESKFIYFVGEGTEQIEDILKRKFSALRIARIDRDTTQKRHTFEETL
                     MDFAEHKIDMLVGTQMLAKGHDFPNVTLVGVVSVDAGLALPDFRSAERTFQLLTQVAG
                     RAGRGILQGRVIIQTFYPEHYALQLACNQDYEKFYQEEIRYRQNLSYPPFAALACVTV
                     HHEDYDYAFSNAQAFKECLVEANGERNARVLGVAPAPLARLKGEHRLQILIKAVNRKK
                     LRELIDFALAGAQENACDLRIINVEIDPVNLL"
BASE COUNT          630 a          731 c          709 g          625 t
ORIGIN      
        1 attaaatcgc aagccattaa ttaaagtgaa aaagcggaaa agtgaaaagg tgaaaaagta
       61 agttgagcaa attcaacgaa attttttcac ttttccgctt tttcactttt ccgctttttc
      121 accttttcac tttctagagt agattcaccg ggtcaatctc gacatttata atccgcaaat
      181 cgcaggcgtt ttcctgcgcg ccggcgaggg cgaaatcaat cagctcgcgc agctttttgc
      241 gattgacggc tttgattaaa atttgcaggc gatgctcgcc tttcagcctt gccagaggcg
      301 cgggcgcgac tcccagaaca cgcgcgtttc tttcgccgtt tgcctcgacc agacattcct
      361 taaaagcctg cgcgttgctg aaagcgtagt cgtaatcctc gtgatgaacg gtgacgcagg
      421 cgagcgcggc aaacggcgga tagctcaaat tctgccggta gcgaatctct tcctgataaa
      481 atttttcgta atcctgattg caggcgagct gcagggcgta atgttcggga taaaaggtct
      541 ggataatcac gcgtccctgc aaaatccctc gcccggcgcg accggcaacc tgcgtcagaa
      601 gctgaaaagt tctttccgcc gagcgaaaat ccggcagcgc caaaccggca tcgaccgaga
      661 ccacgccgac cagcgtcacg ttcggaaaat cgtgaccttt cgccagcatc tgtgtgccga
      721 ccagcatatc gattttatgc tcggcaaaat ccatcagggt ttcttcaaac gtgtgccgct
      781 tctgcgtcgt atcgcggtcg atgcgcgcga ttctgagagc ggaaaatttg cgtttcagaa
      841 tgtcctcgat ttgttcggtg ccttcgccga cgaaataaat aaatttcgac tcgcaaaacg
      901 ggcatttgcg cggcgctttc gcccgaaaac cgcagtaatg acagagcagg acgttttcgc
      961 gcttgtgata agtcaaagta atatcgcagt tctggcagcg aatcgtctcg ccgcacgagc
     1021 ggcacaaaac gaattgggaa aagccgcgcc ggttaagcaa aataatcgat tgctcgcctt
     1081 tagcgaaagt ttcttcaatc gcattcagaa gcggcggcga aaaaatcgca tcgcgccctt
     1141 cttttcggaa aacttcgcgc atatcgacga gctcggcttg ggcgagcggg cggtcgccga
     1201 ttcgattcgg cagatgcaga taaacgtatt tgccgctttt agcgttgtga aacgattcga
     1261 gcgccggggt cgccgagccg agaacaatcg tcgcccgcgc aaaactggcg cgcacgacgg
     1321 cgacgtctct ggcgtgataa aacggcgatt cgtgctgtcg atacgagccg tcgtgttcct
     1381 cgtcgacgat tatcaagccg atatttttca gcggcgcaaa aatcgccgag cgcgttccga
     1441 tggcgattct cgcctgcccg ttgcggattc tgcgccactc gtcaaaacgc tcgcccgtcg
     1501 ataaagacga gtgcaaaatc gccacttcgt cgccgaaaac ggcgcgcaga cggcgcgaga
     1561 aaaccggcgt cagcgcgatt tccggaacga gcatcaaaga agatttgccg gtgtccagag
     1621 cctctttcat cgcccgaatg taaatttccg tcttgccgct gcccgtcacg ccgtgaagca
     1681 gaaaagcgtg ataatcattt gataaaatcg ctttttcgat ttcccgaaac gcgtcggtct
     1741 gccagtcggt caaaatcaaa tccagaattt cgggcagctt ggcgtgcgcc agcgggtcgc
     1801 ggaaaacttc gcggacgaaa acctgcacga tgccgcgttt ttccaggctg ttaacggctg
     1861 acgcgctgat ttgcgctttt tccaaaagct ccgtaaaacc gatttcgccg ttttcgttaa
     1921 tcaaagtttc gacgactttt gcctgcgcct cggtcagcgc tttcgccgcc tgcttgtgca
     1981 tctcggtcgg cagaaggcgc accgatttcc ggcgtttcgg cttggctttg gtcgtcaaag
     2041 tccgctgaaa ggtggaaatc cagttgagtt ttaaaagctc tcgaacggcg cgctgcgtgg
     2101 tcgaagcgcc gaattttttt tgaatctcgc gctgcgaaac ttccgcgttt cccgccagca
     2161 tctgcaaaat ctgcgccttc gacgtgttca gtttcgcgcc ggaaagcttt atcagctcgt
     2221 cgcgaccggc gctggttatc tgcacgattt gctcgatggc ggcgttgact ccggcgggca
     2281 gagacgcttt taaaatttcg ccccacgaag ccgaataata atccgccgtc cactgtgtca
     2341 ggcgcaggat ttccgccgaa accagcggct cttcgtctat caattcgagc acgtctttga
     2401 cggcgtcggc ttcaaattcg atttcttcgc ttaagttttg atgaagcgcg accacatagc
     2461 cggtcagctg gcgattgccg aaaggaacga gcagacgcgc gccgagcttg acggtttccc
     2521 gcagcgccag cggcaaactg taagtgaagg tctggcgcag cggcagcggc aaagctactt
     2581 cggcaaaacg cggcgcgggc gaatctttgg tcaagctcgg aatcattaaa agaaatttac
     2641 cacagaaaaa tttagctaca gaggacacag agaacacgga gaaatgaaac cacag
//
LOCUS       DASWNE010000030         6437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_107269, whole genome shotgun sequence.
ACCESSION   DASWNE010000030 DASWNE010000000
VERSION     DASWNE010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6437
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107269"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VGB68_00630"
     CDS             <1..203
                     /locus_tag="VGB68_00630"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEY0047759.1"
                     /translation="SSKAHVYRAKQTQSETQKNLVSDLLEKAFRGSALRLVQHVLESK
                     PASREELTEIRKLITEAEKKNK"
     gene            200..2227
                     /locus_tag="VGB68_00635"
     CDS             200..2227
                     /locus_tag="VGB68_00635"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEY0047760.1"
                     /translation="MNWEIPINSKIVEALGWTLLHSVWQIALVALILFLLLRVLRKFS
                     ANARYLVSVFALALALALPIVTFVSLNENSSSNFFRKQALAEPDAARFDPRFQTKEDL
                     PLPAKAETSEINADGKNVFESIENLQNFFNKSFSGVLPLVVGLWLCGATFFALRLTGG
                     VWQLHLYKTREISAPAREWQERFSALCEKLEIRKTVKFLRSNLIQTPIVIGWLKPVIL
                     VPASVFLQISAQELETVIAHELLHIRRRDALVNFSQSIIEALFFYHPCVWWISSVLRR
                     EREFATDEAVVKTLENSHIVYASALANLEEIRLLTNQKLPPFSTAAVKGGNLMQRITK
                     ILQKNTETRSEANSAWSAGLALVLISAFFLAVFSLNQPAFVNAQTKTKNRKLAVGFVS
                     LPPVDRTTNPPKDADATARLLIETLKARKIPAIGFVHGAVISDGEKLYPVRADIVRLW
                     RDAGFEIGIGGFNHVWFYDTPYEEYVAGVDKNERITRKLLAEKNLSLRYFSYPFLNTG
                     KTADDRNRFESWLNGRGLTSVKYTIDNQEWMYSYAYDMARNDNDLDTMREIRESFVAY
                     MTKMFDHYEAYSQEQFGRDINQTMVLTPSRLVTDSADELFGMIEKRGYQFVSMEEAQR
                     DEAYRTEESFYGKAGISWFERWQMAKGKKLRDEPDVDAGVQKVWNETKSKK"
     gene            complement(2280..2960)
                     /locus_tag="VGB68_00640"
     CDS             complement(2280..2960)
                     /locus_tag="VGB68_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047761.1"
                     /translation="MKSVVALFVFVLTCSSIAFGQELTRERKLQKIKDNARLIKQLTE
                     EILLPDAKDFAEAKKNNFEVFRIMPREADIEELTPLRVAYYSFTLKANGYAIPEIGLV
                     KNTFKVGFAGADYGFIADLGEMPLAGVSRETGAAIFLAGYKPPVNMPEIRSEQSKSRN
                     YKAGDVEYRDSVPAIAGHTYVLRAITFDRADVLVALKVHRKDADGSLTVFWKLLEQFE
                     KPLIARAE"
     gene            complement(3055..3426)
                     /locus_tag="VGB68_00645"
     CDS             complement(3055..3426)
                     /locus_tag="VGB68_00645"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEY0047762.1"
                     /translation="MSLQEKYQPLIDLANSSGISNLNVTEGEGFIQIEGAAPNAEIKQ
                     ALWDEYNRIDPDYRSGDLVLNVTAPEASTATYTVQSGDNLSKIGEHYGISWQKIFDAN
                     RDKLDNPDKIFPGQELKIPQE"
     gene            complement(3447..3803)
                     /locus_tag="VGB68_00650"
     CDS             complement(3447..3803)
                     /locus_tag="VGB68_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047763.1"
                     /translation="MKFKILAGFSLSAVLLVGACGGNKDTNANTNATNNKAMATATPT
                     PVAQTNEAANIGTGDRTKVEAALKAKGFNNVTVDMTTTPPTLRGSVAKNKMTEAIQTA
                     QEAAGKPFKNELTEAK"
     gene            complement(3983..4495)
                     /locus_tag="VGB68_00655"
     CDS             complement(3983..4495)
                     /locus_tag="VGB68_00655"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEY0047764.1"
                     /translation="MNNEKRIVLTGFMGVGKSTVAKCLAYLLQCEKLDLDKFIEQSEH
                     RSVLQIIESDGEARFREIETEGLRKILMQTEARVISLGGGSWITPQNRELIKAHRCTT
                     VWLESTFEHCWRNISCSKQPRPLAKNKKLAEELFDERQKIYCLADWHFIVEPQLTSFD
                     IAKRIAEEIF"
     gene            complement(4586..5884)
                     /gene="aroA"
                     /locus_tag="VGB68_00660"
     CDS             complement(4586..5884)
                     /gene="aroA"
                     /locus_tag="VGB68_00660"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEY0047765.1"
                     /translation="MKIYPAKQLEGILTLNGDKSISHRAAMISAIADGETRIENFSTS
                     ADCASTLDCLERLGVRIRRENSIVFVKGVGKTGLQKPETELDCGNSGTTVRLLSGILA
                     GQAFETILTGDESLSRRPMKRIIEPLLLFGAQIEATENRLPLRIFGKNPLSSIEYAPA
                     IASAQVKSCVLLAGLNAGAETYFIEKTPTRDHTERMLRWFGAEVESAETKTGKRISVS
                     GASVLTAKDFRVPSDISSAAFFLVAAGCLENSDLTVKAVGLNRTRRAVVDVLKAFGAN
                     VEILNEKEVCNETIGDLRVSGSKNLQTKTGSNIIGGEIIANLIDEIPILAIFGTQLEN
                     GLEIRDAAELRVKESDRIAAIVENLRRMNAAVEEFPDGFRVEKSDLKGARVDSFGDHR
                     IAMAFAVAGLLADGETEITGAESANVSFPEFFRTLESVTK"
     gene            5948..6433
                     /locus_tag="VGB68_00665"
     CDS             5948..6433
                     /locus_tag="VGB68_00665"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0047766.1"
                     /translation="MNSIKNFEPQVSGFNGLRQAKFVTEEKKEIVIPSKIESVDEAVV
                     EATEFASASGFGDEALFAIDMAVREAVVNAVKHGNQLDETKPVEISFSNLDKGFEVII
                     RDFGDGFDIDGIPDPTNPENLLKANGRGILFMRNFMEEVEWFQHPQGGTVVKMCKLRG
                     T"
BASE COUNT         1535 a         1589 c         1628 g         1685 t
ORIGIN      
        1 attcgagcaa ggcgcacgtt taccgcgcga aacagacgca gtcggagacg cagaaaaatc
       61 tcgtctcgga tttgctggaa aaagcctttc gcggctcggc tctgcgattg gttcagcacg
      121 ttctggaatc aaaaccggcg agccgcgaag agctgaccga aattcgtaaa ttaatcacgg
      181 aggcggaaaa gaaaaacaaa tgaactggga aattccgatt aactcaaaaa tcgtcgaagc
      241 tctcggctgg acgctgcttc attctgtctg gcagattgcg ctggtcgcgc tgattctttt
      301 tctgcttctg cgcgttttgc gaaaattttc ggcaaacgcg cgttatctcg tatcggtttt
      361 cgcgctcgcg ctggcgctgg cgctgccaat cgtcactttt gtcagtctga acgaaaattc
      421 atcgtcgaat ttctttcgca aacaagcttt agccgagccg gacgccgcgc gattcgaccc
      481 gcgttttcag acgaaggaag atttaccgct gcctgcgaaa gcggaaacga gcgagataaa
      541 tgccgacgga aaaaacgttt tcgaatcgat tgagaatctg caaaactttt ttaataaaag
      601 cttttccggc gttttgcccc tggtcgtcgg tctgtggctt tgcggcgcga cgtttttcgc
      661 gctgcgctta acgggcggcg tctggcaact gcatctttac aaaacgcgcg agatttccgc
      721 gcccgcgcgt gagtggcagg aaagattttc ggcgctttgc gaaaagctgg aaataagaaa
      781 aaccgtaaaa tttttgcggt cgaacctgat tcagacgccg atcgtcatcg gctggctgaa
      841 gccggtcatt ctcgttccgg cgagcgtttt tttgcagata agcgcgcagg agctggaaac
      901 cgttatcgcg cacgagctcc ttcacattcg ccgccgtgac gcgctcgtca atttctcgca
      961 aagtattatt gaagctttat ttttctatca cccgtgcgtg tggtggattt cttccgttct
     1021 tcgcagggag agagaatttg cgacggacga agcggtcgtc aaaacactgg aaaactcgca
     1081 catcgtttac gccagcgcgc tggcaaatct tgaggagatt cgtcttttga cgaaccagaa
     1141 actgccgcct ttttcgacgg cggcggtaaa gggaggtaat ttaatgcaga gaataacaaa
     1201 aatcttacaa aaaaataccg agacgcgctc tgaagccaat tctgcttggt cggctggttt
     1261 ggctttagtg cttatctcgg cgtttttcct ggctgtattt tcgctgaatc aaccggcgtt
     1321 tgtcaatgcc cagacgaaaa cgaaaaaccg caagctggct gtcggcttcg tttcgctgcc
     1381 gccggtcgac cgcacaacca atccgccgaa agatgcggac gcgacggcgc ggcttttgat
     1441 tgaaacatta aaagcacgca aaattccggc aatcggattc gttcacgggg cggttatttc
     1501 cgacggcgaa aagctttatc cggtacgcgc cgacattgtg cgcctgtggc gcgacgccgg
     1561 ttttgaaatc ggcatcggcg ggtttaatca cgtctggttt tacgatacgc cgtatgaaga
     1621 atacgtcgcc ggcgtcgata aaaacgagcg catcacgaga aagcttctgg cggaaaaaaa
     1681 tctttcgctg cgctatttca gctatccttt tttaaacacg ggcaagacgg cggatgaccg
     1741 caatcgtttt gaatcgtggc tgaacgggcg cggtttgact tcggtcaaat acacgattga
     1801 caaccaggaa tggatgtatt catacgccta cgatatggcg cgcaacgaca acgacctcga
     1861 cacgatgcgc gaaatccgcg aatcgttcgt cgcttatatg acgaaaatgt tcgaccatta
     1921 cgaagcgtat tcgcaggaac aattcgggcg cgacatcaat cagacgatgg ttctgacgcc
     1981 gagccgtctg gtcacggatt cggcggacga gcttttcgga atgattgaaa aacgcggcta
     2041 tcaattcgtt tcgatggaag aagcccagag ggacgaagcc taccggacgg aagaaagctt
     2101 ctacggcaaa gcgggcattt cgtggttcga gcgctggcag atggcgaaag gcaaaaagct
     2161 tcgcgacgag ccggatgtgg acgccggtgt gcagaaagtt tggaacgaaa ccaaatcgaa
     2221 gaaataaagc tttttcgtaa aataaaaaag cctgtgtgat ttcccgcaca ggcttttttc
     2281 tattcggctc tggctattaa tggtttttca aattgttcga gcagcttcca gaagaccgtc
     2341 aggcttccgt ccgcgtcttt tcgatgaacc tttaaagcga ctaaaacgtc ggctctgtcg
     2401 aaagtaatcg cccgcagaac gtatgtgtgt ccggcaatcg ccggaacgct gtcgcgatat
     2461 tcgacgtcgc cggctttata gtttctggat tttgattgct cgctcctgat ttccggcata
     2521 tttaccggcg gtttgtagcc ggcaaggaaa atcgccgcgc cggtttccct gcttaccccg
     2581 gcaagcggca tttcgcccaa atcggcgata aatccatagt cggcgccggc aaagccgacc
     2641 ttgaaagtgt tttttaccag cccgatttca ggaattgcgt aaccgttggc ttttaacgta
     2701 aaagaataat aagcgacgcg cagcggcgtt aattcttcaa tatcagcctc gcgcggcata
     2761 atccggaaaa cttcgaaatt attttttttg gcttcggcaa aatctttcgc gtcgggcagc
     2821 aggatttctt cggttaattg tttgattagc cgggcgttgt ccttgatttt ttgcagtttc
     2881 cgctcgcggg ttaattcctg accgaaagcg attgaagaac acgtcaggac gaaaacgaat
     2941 agcgcgacga ccgatttcat aaaaactcct ttttgtagaa aagggaaagc accttgattt
     3001 gcccgttaga cggaaacaat atcaaagtgc cttctgaaaa gcaataaaat tttattactc
     3061 ttgcggaatc ttcaattcct gaccgggaaa gattttgtcc ggattgtcga gcttatcgcg
     3121 attggcgtca aagattttct gccagctgat gccgtaatgc tcgccgattt tagatagatt
     3181 gtcgcccgat tggaccgtgt aagtggcggt cgaagcttcc ggcgcggtga cgttcaaaac
     3241 gaggtcgccc gaacggtaat ccgggtcgat gcgattgtat tcgtcccaca aagcctgttt
     3301 tatttccgcg ttcggcgcgg cgccttcgat ttgaataaag ccttcgcctt cggtgacatt
     3361 taaattggaa atgcccgaac tgttcgccaa gtcaatcaac ggctgatatt tttcctgtaa
     3421 agacatattt tattttcctc cttgtattat ttcgcttctg ttaattcgtt cttaaacggt
     3481 tttccggctg cttcctgagc cgtttgaatg gcttccgtca ttttattttt cgcgacgctt
     3541 ccgcgcagcg tcggcggagt ggtcgtcata tcaaccgtga cgttgttgaa tcctttagct
     3601 ttcaaagcgg cttcgacttt cgttctgtcg cccgtcccga tgtttgccgc ttcgttggtt
     3661 tgcgctaccg gcgtcggcgt cgcggttgcc atcgccttgt tgttggtagc attggtgtta
     3721 gcatttgtgt ctttgtttcc gccgcacgcg ccgactaaaa gaactgccga taaactgaaa
     3781 ccggctaaaa ttttaaattt catttattcc tcctgaaatt tcctgtaaat taaatttttc
     3841 aagctcgaaa gcgccgccgc tggttttccg tatggattta ccggctttta acgcgtcgct
     3901 tccgagataa cgtaaattcc cgagaaaatt accgtataat tttctaatgt aaatataact
     3961 taacaaattt tcggataaat tatcaaaata tttcttcggc gattcttttc gcgatgtcga
     4021 acgaggttaa ttgcggctcg acgataaaat gccaatccgc cagacaataa attttttgcc
     4081 gctcgtcgaa aagctcttcc gccagttttt tgtttttcgc cagcgggcgc ggctgtttcg
     4141 aacaggaaat attgcgccag caatgctcga aagtcgattc cagccagacg gtcgtgcatc
     4201 ggtgcgcttt gataagctcg cggttttgcg gcgtaatcca gctgccgccg ccgagcgaga
     4261 taacgcgcgc ctcggtctgc atcaaaattt ttcgcaagcc ttcggtctcg atttcacgaa
     4321 aacgcgcttc gccgtccgat tcgatgattt gcaagaccga gcgatgctcg ctttgttcga
     4381 taaatttgtc gaggtcgagc ttttcgcatt gcagcagata ggcgagacac ttggcgacgg
     4441 tcgatttgcc gacgcccata aaacccgtca aaacaatgcg cttttcgttg ttcatcaatg
     4501 aaaaaaaaga tgaatgcgga acgatgaacg atgaattcgt gactgaaaat ctgcaattca
     4561 tcgtttcgcg ttcatcgctt cgcgtttatt ttgtcacact ttccagcgtt cggaaaaatt
     4621 cgggaaacga aacgtttgcc gattccgcgc cggtaatttc cgtttcgccg tcagccagaa
     4681 gtccggcgac ggcgaaagcc atcgcaatcc ggtggtcgcc gaacgaatcg acgcgcgcgc
     4741 ctttcaaatc ggatttttcg acgcggaaac cgtccggaaa ttcttcgacc gccgcgttca
     4801 tccgccgcag attttcgacg attgccgcga ttctgtccga ttccttgacg cgcaattcgg
     4861 cggcgtcgcg aatttccaga ccgttttcca gctgcgttcc gaaaatggcg agaatcggaa
     4921 tttcgtcaat caaattagcg ataatttcgc cgccgattat atttgagccg gttttagttt
     4981 gtaaattttt gctgcccgaa acgcgcaaat cgccgattgt ttcgttgcag acttcttttt
     5041 cgttcaggat ttcgacattt gctccaaacg cttttaaaac gtcgacgacg gcgcggcgcg
     5101 tccggttcaa gccgaccgcc tttacagtca aatcggaatt ttccagacag ccggcggcga
     5161 ccagaaaaaa cgcggcggaa gaaatatccg acggaacgcg aaaatccttc gccgtcaaaa
     5221 cgctcgcgcc cgaaacggaa attcgttttc ccgttttcgt ttcggcagat tcgacctcag
     5281 cgccgaacca tcgaagcatt ctttccgtgt ggtcgcgcgt cggcgttttt tcgataaagt
     5341 aagtttccgc gcccgcgttt aagcccgcca gcagaacgca ggatttaacc tgcgccgaag
     5401 cgatagcggg cgcgtattcg attgaagata aagggttttt gccgaaaatt cggagcggca
     5461 gccggttttc ggtcgcttcg atttgcgcgc cgaaaagcaa aagcggctcg ataattcttt
     5521 tcatcggtcg ccgcgataag ctctcgtcgc cggttaaaat cgtttcaaac gcctgtcccg
     5581 ccagaattcc cgataaaagt ctgacggtcg ttccgctgtt gccgcaatcg agctcggtct
     5641 cgggcttttg caatccggtt ttgccgacgc ctttgacgaa gacaatcgag ttttcgcgcc
     5701 ggattcggac gccgagcctt tccaaacaat cgagcgtcga ggcgcaatcg gcgctggtcg
     5761 aaaaattttc gatgcgcgtt tcgccgtcgg ctatggcgga aatcatcgcg gcgcgatgcg
     5821 agattgattt gtcgccgtta agcgtcaaaa ttccttcgag ctgttttgcc ggataaattt
     5881 tcattgcgag tagttattta tttattgact tatcttgaca taaccgctca taaaccgcta
     5941 atttacaatg aatagcatta aaaattttga accgcaggtt tccggtttta acgggctgag
     6001 acaggcgaaa tttgtgaccg aagaaaaaaa agaaatcgtt attccaagca aaatcgaatc
     6061 ggttgacgaa gccgtcgtcg aggcgaccga attcgcctcc gcctcgggtt tcggcgatga
     6121 agcgcttttt gccattgata tggcggtgcg cgaagcggtc gttaacgccg tcaaacacgg
     6181 caatcaattg gatgaaacca aaccggtgga aatttcgttc agtaacctgg acaaaggctt
     6241 tgaagtaata atccgggact tcggcgacgg cttcgacatc gacgggattc ccgacccgac
     6301 caatccggaa aaccttctga aagccaatgg acgcgggatt ttgtttatgc ggaactttat
     6361 ggaagaagtc gaatggtttc aacatccgca gggcggaacg gtggtcaaaa tgtgtaaatt
     6421 gcgcggaacg tgaaaaa
//
LOCUS       DASWNE010000031         3932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_425076, whole genome shotgun sequence.
ACCESSION   DASWNE010000031 DASWNE010000000
VERSION     DASWNE010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425076"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..634
                     /gene="cyaB"
                     /locus_tag="VGB68_00670"
     CDS             92..634
                     /gene="cyaB"
                     /locus_tag="VGB68_00670"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HEY0047767.1"
                     /translation="MAIEIEKKYRIDNQRRPKIIAKLKELGAEFLREDFEENYLHRGN
                     LLEERNAVLRLRKIGDTAILTYKERIRSNDDIKHKIEFETEVTDVAATENIIEKLGYR
                     LDVVYEKRRQTWHFDKVEVVMDDLPFGLYMEIEGDIDDIAEAEKLLEIEDLEPEPRGY
                     PRLATKFGKQNGKVFEARFD"
     gene            complement(826..2961)
                     /gene="ppk1"
                     /locus_tag="VGB68_00675"
     CDS             complement(826..2961)
                     /gene="ppk1"
                     /locus_tag="VGB68_00675"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEY0047768.1"
                     /translation="MSHNKKHRHLKALKTENAALVVVNESAPVLFNRELSWLEFNHRV
                     LEEAMNDDLPVLERLKFLSIFSTNLDEFFMIRVSGLIEQIEEGIIEPSPDGMTPSQQL
                     REISARLRPMLKKQVSYLHEKVLPELAEAGVTVESYRDLSEKEQKHLDKYFRENLFPV
                     LTPQAVDASHKFPYISNLSLNLGLFIEPDNDHTHKNLKHLFRQKRFARIKLPTNVPRL
                     IPIDEKAGRFTLLGELIAANVHHLFPNMKTGHCYLFRVTRDADLELREDEAGDLLRTM
                     EHELQRRRFGFAVRLEVQSTMPDKMLKVLTEGIGINEQDVYPIDGFLNIPDLMKFYSI
                     GKPQLKDRPIPPVNLPIFQENKSVFEVIKKRDVLLHHPYTAYSTVTDFIREAAEDDDV
                     QAIKICLYRTGKDSPIVESLIRASQLEKQVTALVELKARFDEENNIEWAKRLEDEGVH
                     VVYGIHTLKTHSKIAMVVRREKDQLTRYIHLATGNYNPTTSRIYTDLGLLTCDEEIGA
                     DVTDLFNFLTGYSQQSEYRQLLVAPINLRENLIELIRREAANKLEGKKARIVVKTNSL
                     TDVGLIHALYDASQAGVQIDLIVRGICSLRPGVAGVSENIRVRSIVGRFLEHSRIFYF
                     ANGGDEAVYIGSADWMHRNLDRRVEVVVPIRDASIRQYLKDVVLAAYLRDTVNARILN
                     SDGSYQKISAEDSEPFSSQMFFVGQSTLT"
     gene            3178..3789
                     /locus_tag="VGB68_00680"
     CDS             3178..3789
                     /locus_tag="VGB68_00680"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEY0047769.1"
                     /translation="MDTSKLPPTKKGRNYEIKALGLRDFAGLKREDETLNPFELARYA
                     RLLVVTFEQIEPFLTEETLRHLIVDGKNAWSGGACSQALPDGRKLIVLNPTHGENRQN
                     ATLMEEICHVFLGHKPSRLAIKNVNKYGQPIAREYQAEVEEEAYSVGAAALVPYAALR
                     RMVNQGKTSREIARHFNVSRELVEYRIKVSRLWDEYSRKVVSG"
BASE COUNT         1019 a          966 c         1038 g          909 t
ORIGIN      
        1 ggcatcaaag gcttcggcga aaaacgcgcg cagaaatacg gcgaggaaat tttgaatatt
       61 atcggcgcag actctacaat ctaaactcat aatggcaatt gaaatcgaaa agaaatatcg
      121 tatcgacaac cagcggcgac cgaaaataat cgcgaagctg aaagagctcg gagccgagtt
      181 tctgcgcgag gactttgagg aaaattatct tcatcgcggg aatttgctcg aagaaagaaa
      241 cgccgtcctg cgtctgagaa aaatcggcga cacggcgatt ttgacttaca aggagcgcat
      301 ccgcagcaac gacgacatca aacacaaaat cgaattcgag accgaagtca cagacgttgc
      361 cgcgacggag aacatcatcg aaaagctcgg ctaccggctc gacgtcgtgt atgagaaacg
      421 gcggcagacg tggcatttcg ataaagtcga agtagtgatg gacgaccttc cgttcgggct
      481 ttatatggaa atcgaaggcg acatcgacga catcgccgaa gcggaaaaac tgctcgaaat
      541 cgaagacctc gaacccgagc cgcgcggcta tccgcgcctg gcgacaaagt tcggaaaaca
      601 gaacggcaag gtctttgaag ccagattcga ttagcggcta tctcaaaata cacgcaaaaa
      661 ccggcggtcc ggcgaatgac tccgctgagc cgtaatcttc agatgttgcc aaaggagcgg
      721 ctaattctac atagaattag ccgctccttt ggcatttttg cagcctataa aaggaaatgt
      781 ataaccaaaa ccttggtttc aaaattcaaa gctcgaaatt taaaactagg ttaaagtgct
      841 ctgaccgacg aagaacatct gcgagctgaa cggctcggaa tcttcggcgg aaattttttg
      901 ataagagccg tccgaattta aaattcgagc gttgaccgtg tcgcgcaggt aagcggcgag
      961 cacgacgtct tttagatact gcctgatact ggcgtcacgg atcggcacga cgacctcgac
     1021 gcggcggtcg agattgcggt gcatccaatc ggcgctgccg atataaaccg cttcatcgcc
     1081 gccgtttgcg aaataaaaaa tccggctgtg ttccagaaat cgcccgacga tggagcgcac
     1141 gcgaatattc tccgagacgc cggcgacgcc cgggcgcagg ctgcagattc cgcgcacgat
     1201 caaatcgatt tgcacgcccg cctgcgaggc atcatagagc gcgtgaatta atccgacgtc
     1261 ggtcagactg ttggttttga cgacgattcg cgcttttttt ccttccagct tgtttgccgc
     1321 ttcgcgccgg ataagctcaa tcagattttc gcgcagattt atcggcgcga ccagcagctg
     1381 gcgatactcg gattgctgcg aatagccggt cagaaaattg aaaaggtcgg ttacgtccgc
     1441 gccgatttcc tcgtcgcagg tcagcaagcc caaatcggtg taaatccgcg aagtcgtcgg
     1501 attgtagttg ccggtggcga gatggatgta gcgcgtcagc tggtcttttt cgcgcctgac
     1561 gaccatcgcg attttggaat gcgtcttcaa agtgtgtatg ccgtagacga cgtgaacgcc
     1621 ttcgtcttcc agccgcttcg cccattcgat gttgttttcc tcgtcaaaac gcgcttttaa
     1681 ctcgaccagc gccgtcacct gcttttccag ctggctggcg cgaatcagcg attcgacaat
     1741 cggcgaatct ttgcccgtgc ggtagagaca gatttttatc gcctgcacgt cgtcgtcttc
     1801 ggcggcttca cggataaaat ccgtcacggt gctatacgcc gtgtacgggt gatgcagcag
     1861 gacgtcgcgc tttttgataa cttcgaaaac gcttttgttt tcctgaaaaa tcggcagatt
     1921 gaccggcgga atcggtctgt ctttgagctg cggcttgccg attgagtaaa atttcatcaa
     1981 atccggaatg ttcagaaaac cgtcgatcgg gtaaacgtcc tgttcgttga tgccgatgcc
     2041 ttcggtcagg acttttagca ttttgtccgg catcgtgctc tgcacttcca gccgcacggc
     2101 gaaaccgaaa cgccggcgct gcagctcgtg ttccatcgtg cgcagaaggt cgcccgcttc
     2161 gtcctcgcgc aattcgaggt cggcgtcgcg cgtgacgcga aacagataac agtgcccggt
     2221 tttcatattc ggaaacaggt gatgcacatt ggcggcgatt aattcgccga gcagcgtaaa
     2281 acgtccggct ttttcgtcaa tcggtatcag gcgcggaacg ttcgtcggca gcttgatgcg
     2341 ggcaaagcgc ttttgccgga aaaggtgttt cagattttta tgcgtgtggt cgttgtccgg
     2401 ctcgataaac aaaccgagat tcaggctgag attggaaata tacggaaatt tgtggctggc
     2461 atcgaccgcc tgcggcgtca gaacgggaaa cagattctcg cgaaaatatt tgtccagatg
     2521 cttttgttcc ttctcggaaa ggtctctgta ggattcgacc gtgacgcccg cctcggcgag
     2581 ctcgggcaag actttttcgt gcagatagga aacctgtttt ttgagcatcg ggcgcaggcg
     2641 cgcgctgatt tcgcgaagct gctgcgacgg cgtcatcccg tcgggcgaag gctcgatgat
     2701 gccttcttca atctgttcga ttaaacccga aacgcgaatc ataaaaaatt cgtcgagatt
     2761 ggtggaaaat atagaaagga atttcaggcg ttcgagcacc ggcaaatcgt cgttcatcgc
     2821 ttcttccagg acgcgatgat taaattccag ccagctcagc tcgcggttaa ataaaaccgg
     2881 cgcgctttcg ttgaccacga cgagggcggc gttttccgtt tttagggctt tcaggtgtcg
     2941 atgtttttta ttatgactca tacaaaagag aataatatca ggttaatcga gagaacagag
     3001 ataaacatta agctttcaag cgtgactaag tgtaaactcg ctcctgcatt aacctaagat
     3061 tataaggcaa atcgcggggc aagatgttaa atcgatgtaa cattcgaaaa aattcgtgcc
     3121 gaaaaatcgt atagccgctg gcactattta agcgaaattt gttatataat tcaacgcgtg
     3181 gatacttcaa agcttccgcc gacgaaaaaa ggcagaaatt acgaaataaa agcgctcggc
     3241 ttgcgcgatt ttgccggttt aaagcgcgag gacgaaactt tgaacccgtt tgagctggcg
     3301 cgctacgcca ggcttttggt cgtcaccttc gaacaaatcg agcctttttt aaccgaagaa
     3361 acgctgcggc atttaatcgt cgacggcaaa aacgcgtggt cgggcggcgc ctgctcgcaa
     3421 gctttgccgg acggcagaaa gcttatcgtt ttaaatccga cgcacggcga aaatcgccag
     3481 aacgcgacgc tgatggaaga aatctgccac gtctttctcg gtcataaacc gagccgtctg
     3541 gcgataaaaa acgtcaataa atacggtcag ccgattgcgc gcgaatacca ggcggaagtc
     3601 gaagaagaag cttattcggt cggcgcggcg gcgctcgtcc cgtatgcggc gctgcgccgg
     3661 atggtcaatc agggcaaaac atcgagagaa atcgcgcggc atttcaacgt cagccgcgag
     3721 ctggtcgaat atcgaatcaa agtctcgcgc ctgtgggacg agtattcgag aaaagtcgtc
     3781 agcggctaag aattcaagat tcaaaattta ggattcagga ttggtgttta gcaacggcaa
     3841 atcctgaatt ctgaatccta aatttctaat caaaaatgac tgaaccagtt aatcaaaata
     3901 cagcgataaa caaaaatctg agcgtcgtcg at
//
LOCUS       DASWNE010000032         1869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_212973, whole genome shotgun sequence.
ACCESSION   DASWNE010000032 DASWNE010000000
VERSION     DASWNE010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212973"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VGB68_00685"
     CDS             complement(<1..386)
                     /locus_tag="VGB68_00685"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEY0047770.1"
                     /translation="MSAVPKTLLSAKDYLDFERKAEFRHEFYKGEIFQMSGASREHNI
                     LVTNLVTSANSQFRGKSCETYSNDMRVHIPATGLFTYPDVVIACGKPQFLDDEFDTLL
                     NPIVLIEVLSPSTADYDRGKKFWHYQ"
     gene            complement(396..1472)
                     /gene="prfA"
                     /locus_tag="VGB68_00690"
     CDS             complement(396..1472)
                     /gene="prfA"
                     /locus_tag="VGB68_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003202249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEY0047771.1"
                     /translation="MLEKLEQIEKSYDELTAQISSPEIMSDMSVYAKTMKQHSSLGEI
                     VGKFREVRKMQEELAGAKELLEMADDDDMREMAQMEIDDIEARLPKAEEALRVLLLPK
                     DPNDEKNVILEIRAGTGGDEATLFAGEILRMYARYAERQNWKFDVMDVSDTGVGGIKE
                     AVVLIEGDHVYSKMRYESGVHRVQRVPQTETSGRIHTSAITVAVLPEAEEVDVQINQN
                     DLRIDTFCSSGPGGQSVNTTYSAVRLTHLPTGVVVSMQDEKSQIKNREKAMRVLRARL
                     QEIEEQKQHDALASERKSMVGSGDRSEKIRTYNYKENRVTDHRIGLTIYQLHLFMEGM
                     IDEVIDALVSHFQAEKLKAETVAA"
     gene            complement(1477..>1869)
                     /locus_tag="VGB68_00695"
     CDS             complement(1477..>1869)
                     /locus_tag="VGB68_00695"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEY0047772.1"
                     /translation="DLTVENARVQKRQHPRCGTSFLMVVMLVAIVLFSVIKFDALWLN
                     LLVRILLMPLVAGLSYEIIRYAAKKESGVIFKFMTLPGLWLQNITTQNPDDDQLEVAI
                     VSLKESLKLEPIDTEKVPAANRELEVVV"
BASE COUNT          409 a          494 c          467 g          499 t
ORIGIN      
        1 ctttgataat gccagaattt tttgccgcgg tcgtaatcgg cagttgacgg cgaaagaact
       61 tcaatcaaga cgattggatt taaaagcgtg tcgaattcgt cgtccagaaa ctgcggcttg
      121 ccgcaggcga taacgacatc cgggtaagtg aaaagtccgg ttgcgggaat gtgaacgcgc
      181 atatcatttg aatacgtttc acaggatttc ccgcgaaatt gcgagtttgc gctcgtcacc
      241 agattggtta cgagaatatt atgctcgcgg ctcgcgcccg acatttgaaa aatctcgcct
      301 ttgtaaaatt cgtgacgaaa ctccgccttg cgttcaaaat caaggtaatc tttggcgctc
      361 aaaagagttt tcggaacggc tgacataaaa tttttttacg cggcgactgt ttcggctttc
      421 agtttttcgg cttgaaaatg cgagaccagc gcgtcgataa cttcgtcaat cataccttcc
      481 ataaaaaggt ggagctggta aatcgtcaag ccgatgcggt gatcggtgac gcgattttcc
      541 ttataattgt aagtgcgaat tttttccgag cggtcgccgc tgccgaccat cgatttacgc
      601 tccgacgcca gcgcgtcgtg ctgtttctgc tcctcgattt cctgaagccg cgcgcgcaat
      661 acgcgcatcg ctttttcgcg gttttttatc tgcgattttt cgtcctgcat cgagacgacg
      721 acgccggtcg gcaagtgcgt caggcgaacc gccgaatacg tcgtgttgac cgactgaccg
      781 ccgggaccgg acgagcagaa agtgtcgatg cgcaaatcgt tttgattgat ttgcacgtca
      841 acttcttcgg cttccggcag aaccgcgacc gtgattgccg aggtgtgaat gcgaccggaa
      901 gtctccgtct gcggaacgcg ctggacgcgg tgaacgccgg attcgtaacg cattttcgaa
      961 taaacgtggt cgccttcaat caggacgacg gcttccttaa taccgccgac gcccgtgtcg
     1021 gaaacgtcca tcacgtcgaa tttccagttt tggcgctcgg catagcgcgc gtacattcgc
     1081 aggatttcgc ccgcaaagag cgtcgcttcg tcgccgcccg ttccggcgcg gatttcgaga
     1141 atgacattct tctcgtcgtt cgggtctttc ggcagaagca aaactctcag cgcttcttcg
     1201 gctttcggca ggcgcgcttc gatatcgtcg atttccattt gcgccatttc gcgcatatcg
     1261 tcgtcgtccg ccatttccag aagctctttc gcgcccgcga gttcttcctg catttttctg
     1321 acttcgcgga attttccgac gatttcgccc agactgctgt gctgtttcat cgtcttggcg
     1381 taaaccgaca tatccgacat aatttcgggc gaggaaatct gcgcggtcag ctcatcgtaa
     1441 cttttttcga tttgttcaag tttttccaac ataaaattaa acgactactt ctaattctct
     1501 attcgccgcc ggaacttttt ccgtatcgat cggctctaat ttgagcgatt ctttcagcga
     1561 aacgatggcg acttccagtt ggtcgtcgtc cggattctgc gtcgtgatat tttgcagcca
     1621 aagtccgggc agcgtcataa atttaaaaat tacgcccgat tcctttttcg ccgcgtaacg
     1681 aatgatttca taggaaagtc cggcaaccag cggcatcagc agaattctga ccagaaggtt
     1741 cagccacagc gcgtcgaatt taatcaccga aaacaaaacg atggcgacca gcatcacgac
     1801 catcagaaac gatgtgccgc agcgcgggtg ctggcgtttt tgaacgcgtg cgttttcgac
     1861 cgtcaaatc
//
LOCUS       DASWNE010000033         3932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_107322, whole genome shotgun sequence.
ACCESSION   DASWNE010000033 DASWNE010000000
VERSION     DASWNE010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107322"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VGB68_00700"
     CDS             <1..283
                     /locus_tag="VGB68_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY0047773.1"
                     /translation="YLDPAISDKVFDIIAAKQSRQRGEIREAILSDREAEVLRLIARG
                     YSNKEIAAQLETSVKTVESQKAVAMQKLNISGRNEIVSYAILQGWLREN"
     gene            699..848
                     /locus_tag="VGB68_00705"
     CDS             699..848
                     /locus_tag="VGB68_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047774.1"
                     /translation="MKIILFSLYYFKLRLGAMLGFRRTFNPLDLKWRWNDWQQRRARQ
                     RRQRF"
     gene            871..1176
                     /locus_tag="VGB68_00710"
     CDS             871..1176
                     /locus_tag="VGB68_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047775.1"
                     /translation="MNKRVGTGQTNQRLESYTILQRVGQAESGAIEDCVRTYGNLVWA
                     LAKKFAKSSEEAEKAAADIFKDIWARAAFYDLAKCTEEKYILQIAIRRLIKPSAGRQ"
     gene            1601..3256
                     /locus_tag="VGB68_00715"
     CDS             1601..3256
                     /locus_tag="VGB68_00715"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HEY0047776.1"
                     /translation="MRKTTRTLFTMAIAAVTASSLLLSEFRGTQVAKASSHREAPLIA
                     LDQSADNTDVYAFVSPDRANTVTLIANFIPLEEPSNGPNFYKFDDNALYQIRIDNNGD
                     ARLDLALQFRFRTVVGNGNTFLYNTNTIDNLSDPDWNVKQFMDVTARIRQGDPNNPTF
                     TTFNLGTNLPTPPVNIGPRSTPNYETNLAEASISTVPTGVGDVKVFAGQRDDSFYIDT
                     GSGFDLLGLRPFNSAHLAPLPNSNGVDFLAGYNVHTIAIQVPTQLLTATGQLPTGASD
                     PNAIVGVYSTVSRPGTRVLNTNGTATTTGDCSVGLTPDNNCVQVSRLANPLVNELFIP
                     RGTSETVSENDKDLYNAQLPATDINRDVYVRGSAQRPVEPVALINLLYPPVADAPTAG
                     RNDLVRVFLTGVPGATRPPVVADPNDPGAGLGAVPSDLMRLNVAVPATPFGSQNRLGF
                     IAGDLAGYPNGRRPGDDVTDISFRVAAGVLLPGNACNNGTTNCNQAPNNQLGDGVNEN
                     DRMFRMSFPYLASPWSGYENPYHGRTGSADTTTPGTTMATPRP"
     gene            3464..>3932
                     /locus_tag="VGB68_00720"
     CDS             3464..>3932
                     /locus_tag="VGB68_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047777.1"
                     /translation="MAVSKTAASRPEAGLLQPFKTNQATVTDRNIKAAQGIIEQKPDN
                     PTGYNMLAAAFMQKARETGDFSLNARAEESLKHSFRVAPENYEATKLQAALLLNYHRF
                     AEALQAAQRAQKINPRDHDVYGAMVDALVELGDYKSAVDAAQKMVDLRPNTASY"
BASE COUNT          980 a          991 c         1015 g          946 t
ORIGIN      
        1 ttatctcgac ccggcgattt cggataaagt tttcgacatc atcgcggcaa agcagtccag
       61 gcagcgcggc gagattcgcg aggcgatttt gagcgaccgc gaagcggagg tgctgcgttt
      121 aatcgcgcgc ggctacagca acaaggaaat cgccgcccag ctcgaaacca gcgttaaaac
      181 cgtcgaatcg caaaaagccg tcgcgatgca aaaactcaat atcagcggtc gcaatgaaat
      241 cgtcagctac gccattctcc agggctggct ccgtgaaaac tagcgacatt gacaaacgac
      301 cttcgccgct gccgccgctt caaatcaatc ttcaatctta ttttttttaa ttaaagggtt
      361 tttccgcaaa aatgtcaggg aaaacccttt tttatctttc tcatcttcct gatatatgta
      421 ttttttttta tgtgtgataa ataacataca cgtttactga ggtaagttaa gatttttact
      481 taagcaaaag cctttcgtct aatttagaaa gaaatcgcgc gggcggcggc aaatcttaag
      541 cttggagaga gactctgccg gttcggctct tcttccttta actctatctt attttgcctt
      601 tgtaaatgtc gattttaaat cttgaattca tctttttcgg agttgtatct ggttttattt
      661 tctgctccgg aagcgaattt tcgggtattt agcgacatat gaaaataatt ttattttctc
      721 tttattattt caagctgcgt ttgggcgcga tgctgggttt tcgccggact tttaatccgc
      781 tggacttaaa atggcgctgg aacgactggc agcagcggcg cgccagacag cgccgacagc
      841 gtttttagaa gaagaaaggg tcggggattt atgaacaagc gggttggcac cgggcagacg
      901 aatcaaaggc tggagtctta tacgattctc cagcgagtcg gtcaagccga aagcggcgcg
      961 atcgaagatt gcgtccgcac ttacgggaat ttggtttggg ctctggcaaa aaagttcgcc
     1021 aaatcttcgg aagaagccga aaaggcggcg gcggacattt ttaaagacat ctgggcgcgg
     1081 gcggcatttt acgatttggc gaaatgtacc gaagaaaagt acatcctgca aattgcaatc
     1141 cgccgcttga ttaaaccgtc agccggcagg caataaacgt tttcccgggg cggcggtcgg
     1201 cgaattttga aagctctgca agccggcggc aaaaaagccg gacttaacag gctttaacgg
     1261 aattttaacc gaatttcctt ttgataatta aattttgtca aaatgtgcat ccgaattgcg
     1321 ccggcgggcg aattgtttat caaagggata ctggatttaa aagtttgcga cttaaaaatt
     1381 aagaaaattt tcttttcctt tcttcattgt tgcttgggtt tggaatggct gaacgatttt
     1441 atttagttgg gtcgcaaacg aaatttcccg ctcgaatgcg ggcggtaagg tcaaagttta
     1501 aaaggtatcg aagtgtcacc ttactcagcc gggatttaag ctttgacagt gtcgagctga
     1561 gcaaacacta gaaaatatta gaacattggg aggatagtaa atgcgaaaaa caacgcgaac
     1621 actttttacg atggcaatcg ccgccgtaac tgcttcgtca ctactattaa gcgaatttcg
     1681 cggaacacag gtggcaaaag catccagtca ccgcgaagcg ccgttaatcg ctctggacca
     1741 atcggcggat aacacggacg tttatgcgtt cgtcagcccc gacagagcga acacggttac
     1801 cttgatagcc aactttatac cgcttgaaga gccttcaaac ggtcccaact tttacaaatt
     1861 tgacgacaac gcgctctatc aaatcaggat agacaacaac ggcgacgcga gactcgattt
     1921 agctctgcaa tttcgctttc gcacggtcgt cggtaacggg aacacgtttc tctacaacac
     1981 gaacactatt gacaaccttt ccgatccgga ctggaacgtc aaacaattta tggacgtaac
     2041 ggctcggatt cgtcagggcg acccgaataa tccgactttt accacattca atctgggaac
     2101 gaatctgccg acgccgcccg tcaatatcgg accgcgctcg acgccgaatt acgagacaaa
     2161 tctggcggaa gcttcgatca gcacggtgcc gacaggcgtc ggcgacgtta aagtttttgc
     2221 cggtcagcgc gacgacagtt tttacatcga caccggttcg ggctttgacc tgctgggctt
     2281 gcgaccgttt aactccgcgc atttagctcc gctgccgaat tcgaacgggg tggatttttt
     2341 agccggttac aacgttcata cgattgctat tcaggttccg acccagttgc tgacggcaac
     2401 cggtcagctt ccgaccggag ccagcgaccc gaatgcaatt gtcggcgttt attcgacggt
     2461 cagccgtccg ggaacgcgcg ttctgaacac aaacggaacg gcgaccacta ccggggactg
     2521 ctcggtggga ctcacgcctg acaacaattg cgtgcaggtt tcgcgtctgg cgaatccgct
     2581 ggtcaacgaa ttgtttatcc cgcgcggcac ttcggaaacc gtttccgaaa acgacaagga
     2641 tttatacaac gctcagttgc cggcaaccga tatcaatcgc gacgtttacg tgcggggttc
     2701 cgcacagcgt ccggtcgagc ccgtcgcttt gattaatctg ctgtatccgc cggtcgccga
     2761 tgcgccgact gcgggtcgca acgatttggt cagagtgttt ttaacgggcg tgccgggagc
     2821 gactcgtccg ccggtggtcg ccgacccgaa cgacccgggc gcggggctcg gcgctgttcc
     2881 gtctgatttg atgcggctta atgtcgccgt tccggcgact ccgttcggtt cgcagaatcg
     2941 cttaggcttt atcgccggag atttggcggg ctacccgaac gggcgacgac cgggcgacga
     3001 cgtgactgac atcagcttcc gcgtagccgc cggcgtcctg ctgccgggaa atgcctgtaa
     3061 taatggcacg accaactgta atcaggcgcc gaacaatcaa ttgggcgacg gcgtgaacga
     3121 aaacgacaga atgttccgta tgtcgttccc gtatctggca tcgccctgga gcggatacga
     3181 aaatccgtat cacggacgca cgggctcggc tgacaccacg acgccgggaa cgaccatggc
     3241 gacaccgcgc ccgtaaaggc gaaactaacg gtaaagaagg ctgatgcggt ttttttagcc
     3301 ttctttagtt tcaaaaagaa gtaagaaata aaattcgtgg gcgatgtttg aacttgaaac
     3361 gccgctcacg aatttatatt cattgtttcc aaacgatatg cgtaaaaatt tcctgacaat
     3421 ctttggcggc gtctttatcg gaattgttgt agcgttaata gttatggcgg tttcaaaaac
     3481 agccgcatcg cgacccgaag ccgggctttt acagcccttc aaaaccaatc aggcgacggt
     3541 taccgaccgg aatatcaaag cggctcaagg cattatcgaa caaaagcccg ataatccgac
     3601 cggatacaat atgctcgccg ccgcgtttat gcagaaagcg cgcgagaccg gagatttcag
     3661 tttaaacgcg cgcgccgaag aatcgctcaa acattctttc cgcgtcgcgc ccgaaaatta
     3721 cgaagcgacg aagctgcagg cggcgctgct gcttaattac caccgcttcg cggaagcgct
     3781 ccaagccgcg cagcgcgccc agaaaattaa tccgcgcgac cacgacgttt acggcgcgat
     3841 ggtcgatgcg ctggtcgagc tgggcgatta caaaagcgcc gtcgacgccg cgcagaagat
     3901 ggtcgatttg agaccgaata cggcttctta tt
//
LOCUS       DASWNE010000034         4203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_212991, whole genome shotgun sequence.
ACCESSION   DASWNE010000034 DASWNE010000000
VERSION     DASWNE010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212991"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2297
                     /locus_tag="VGB68_00725"
     CDS             <1..2297
                     /locus_tag="VGB68_00725"
                     /inference="COORDINATES: protein motif:HMM:NF022113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HEY0047778.1"
                     /translation="VLGTVTYMSPEQARGKEVDERTDIWSLGVVLYEMVTGRNPFAGE
                     TVSDSLAALIHVEPEPLDAFVEDVPEELQWIVRKTLRKKADDRYQSIKDLALDLKDLR
                     YKFEHGDGENRTRKFSAYNTKTAAFGRISTGENKTLIHDTTSAGKLRDRKKTEANKTQ
                     VYSTRRRGASWRFLPLIVLGLAAVLALGAWYYQPFSPKEIAVKFDNVQISRLTENGKS
                     SAPAISPDGKYVVYVNNENAMRSLVVRQIATGSNVQIVPPSNLVFYQPAFSPDGDYIY
                     YILNDKGVGTLYQIPTLGGTAKKIIVDVDSKVSFSPDGKRLAFIRHDVDKGNSTVYLI
                     NADGTQPEVLIDTGQSGETRFSEVAWSPSGEEVLVGSIIKMFGDDSYEGKILLVSLKD
                     KSSRQFGERTWMSLGSFSWLKDKSGFYFLAKASVQDQKQIWFFSYPDQQVRQITSDSS
                     GYSVMSISADGATIAASKVDIISSLWSFNPQNKEMTQVVSENRMMQGVGGLMHLPDGR
                     ILVAKTEGTRTNLWTMDEEGRNEKVLMSEGGDNIHPAISPDGKFLVFSSNYQDGWKIW
                     KADIDGKNAVRLSRQASMGDFRPQVLPDNKTVVFERRLDNIMKAKLMKVSIDGGDEQP
                     VFGESQTSDAFPNVSDDGKRLAFTTQLFDAKNLTFQNILKIAEIKETGVEPPKEAKIG
                     NISWNYRWAKDGKTLTYMGVEGIPNIFDFAVDGNNSKPRQLTNFNSGMILNFDWTNDG
                     KRLFIVRGIFNSDLILIKDNAKAT"
     gene            complement(2442..2861)
                     /locus_tag="VGB68_00730"
     CDS             complement(2442..2861)
                     /locus_tag="VGB68_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0047779.1"
                     /translation="MCAASVFLIKEVLYKAGNNMPKTVLVVEDYTDVRTMMKILIKRH
                     GYEVVEAADGYEAVEKTVRFHPDLILMDLALPIMDGVTATKIIREIEGFDNVSIVALT
                     AYGNTSFDKAIEAGFDNVLIKPLHFENLESFLNQYLS"
     gene            3059..>4203
                     /locus_tag="VGB68_00735"
     CDS             3059..>4203
                     /locus_tag="VGB68_00735"
                     /inference="COORDINATES: protein motif:HMM:NF022449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0047780.1"
                     /translation="MATISFYGGVGSVTGSKYLLEHDGKKVLIDCGLFQGEKQLRERN
                     WQEPPFDPRDLDAVVITHAHIDHTGYLPRIVRLGYDKKVFTSDGTADLLKILLPDSGR
                     LQEEDAEYRNRKGLTVHRPALPLYDETDAKDALKLLNPVPNNGEPVEICEGFSAAFYV
                     AGHIMGASLVLIEIAGAKENGEPLRFLFSGDLGHYDQPIIKDPAIPVECDYLMVESTY
                     GNRLHGDVASDTQLAEIINEAFRRNAPILIPAFAVGRTQELLYLIRELEDDGKIPILP
                     VVVDSPMAAQATQVYNRWHDEHDEEYASILMQKRHPLRTGSMTTASTREDSKRLNDMK
                     GARIIISASGMLTGGRVLHHAMRILPNENATIVFVGYQAAGTIGRRI"
BASE COUNT         1106 a         1021 c         1115 g          961 t
ORIGIN      
        1 tcgtcctggg aacggtcacg tatatgtcgc cggagcaggc gcgcggcaag gaagtcgacg
       61 agcgaacgga catctggagt ctcggcgtcg tgctttacga aatggtgacg ggcaggaatc
      121 cgtttgcggg cgagaccgtc agcgattcgc tcgccgctct gattcacgtc gagccggaac
      181 cgctcgacgc tttcgtcgaa gacgttcccg aagaattgca gtggattgtc cgcaaaactc
      241 ttcgaaaaaa agccgacgac cgctaccaga gcatcaagga tttggcgctc gatttaaaag
      301 atttgcgcta caagtttgag cacggcgacg gagaaaaccg gacgcggaaa tttagcgctt
      361 acaacacaaa aacggcggct ttcgggcgca tcagcacggg cgaaaacaaa acgctgattc
      421 acgacacaac gtcggcggga aaattgcggg acaggaaaaa aaccgaggca aataaaactc
      481 aggtttattc gacgcggcgg cgcggcgcga gttggcgttt tctgccgctg atagttctgg
      541 gtctggcggc ggttctggcg ctcggcgcgt ggtattacca gcctttttct ccgaaagaaa
      601 tcgccgtgaa attcgacaat gtgcagattt cgcggcttac ggaaaacggt aaatcgagcg
      661 cgccggcaat ttcgccggac ggaaaatacg tcgtttacgt caataacgaa aatgccatgc
      721 gcagtctggt cgtcagacaa atcgcgaccg gcagcaacgt gcagattgtt ccgccgtcga
      781 atctggtttt ttaccagccg gcgttttcgc ccgacggcga ttatatttat tacatactga
      841 atgataaagg cgtcgggacg ctctatcaaa tcccgacgct cggcggcacg gcgaaaaaaa
      901 tcatcgtcga tgtcgattcg aaagtgagtt tttcgccgga cggaaaacgc ctcgctttta
      961 ttcgccacga tgtcgataaa ggcaacagca cggtttattt gattaatgcg gacggcacgc
     1021 agccggaagt tttgattgac accgggcaaa gcggcgaaac gcgtttttcg gaagtcgcct
     1081 ggtcgccgag cggcgaagaa gttctggtcg gctcgattat caaaatgttc ggcgacgatt
     1141 cgtacgaggg gaaaatcctg ctcgtttcgc tcaaagataa aagctccaga caattcggcg
     1201 agcgaacgtg gatgagcctc ggttcttttt cgtggctgaa agataaatcg ggcttttatt
     1261 ttctggcgaa ggcgagcgtg caggaccaga agcaaatctg gtttttttcc tatcctgacc
     1321 agcaggtcag acaaatcacc agcgattcga gcggttattc cgtgatgagc atctcggcgg
     1381 acggcgcgac gattgccgct tcgaaagtcg atattatttc cagcctctgg tcgtttaatc
     1441 cgcagaataa ggaaatgacg caggtcgttt ctgaaaaccg tatgatgcag ggcgtcggcg
     1501 gcttgatgca tctgccggac gggcgaattt tggttgccaa aaccgagggc acgcgcacca
     1561 atctgtggac gatggacgaa gaaggcagaa acgaaaaagt tttgatgagc gaaggcggcg
     1621 acaatatcca tccggcaatc agcccggacg gaaaattcct tgttttcagc tcgaattatc
     1681 aggacggctg gaaaatctgg aaagccgaca tcgacggaaa aaacgccgtc cggttgagcc
     1741 ggcaggcttc aatgggcgat tttcgcccgc aggttttgcc cgacaataaa accgtcgttt
     1801 tcgagcgccg cctggacaac attatgaaag cgaaattgat gaaggtttcg attgacggcg
     1861 gcgacgaaca gccggttttc ggcgagagcc agacgagcga cgcgtttccg aacgtctccg
     1921 acgacggaaa gcgcctggct tttacgactc agcttttcga cgcgaaaaat ctgacttttc
     1981 aaaacatttt aaagattgca gagataaagg aaacgggcgt cgagccgccg aaagaagcga
     2041 aaataggaaa tatcagctgg aattaccgct gggcgaaaga cggcaaaacc ctgacctata
     2101 tgggcgtcga gggcatcccg aatatttttg atttcgccgt tgacggcaac aacagtaaac
     2161 ccagacagtt gaccaatttt aattccggta tgattctgaa tttcgactgg acaaacgacg
     2221 gcaagcgcct ttttatcgtg cgcggcatct tcaacagcga tttgattttg ataaaagaca
     2281 acgccaaagc gacttaaggc ggaagcgctt ctttacaatt acgaattacg aattacgaat
     2341 tacgaatgaa agattatttc ttctaattcg taattcgtaa tttgtaattc gtaattaaag
     2401 aaagcgtttc cgccttttaa aattacctgt tcagaaaagc ttcaggaaag atactgattc
     2461 aaaaaagatt cgagattttc aaaatgaagc ggtttgataa gcacgttgtc aaatcccgcc
     2521 tcgatggctt tatcaaaaga agtgtttccg tatgccgtca gggcgacaat cgaaacatta
     2581 tcaaaacctt cgatttctct gataattttc gtggcggtca cgccgtccat tatcggcagc
     2641 gccaaatcca tcagaataag gtccggatga aaccggacgg ttttttcgac cgcctcgtaa
     2701 ccgtccgctg cctcgacgac ttcgtaaccg tgtcgtttaa tcagaatctt catcattgtc
     2761 cgaacatctg tataatcttc aacaactaga acggttttcg gcatattatt acccgcctta
     2821 tacaaaactt cttttatcaa gaatacagaa gcagcgcaca tagttcaaca agtttttaca
     2881 taattgacgg ttttattgtg gttttctaat taaatttccg aaaagcctgc tgaatttgag
     2941 aattaaattt taagcaggaa ccgttttcga aagctttgag cggaagattt ggagtttggc
     3001 tgtaaataat agaaaattat attgttgtac tcaaaatttg atggcgcgga gaaaaaaaat
     3061 ggcgacaatt tccttttacg gcggagtcgg cagcgtgacc ggctcaaaat atctgctcga
     3121 acacgacggc aaaaaagttc tcattgattg cgggcttttt cagggcgaaa aacaattgcg
     3181 cgagcgaaac tggcaggagc cgccgttcga cccgcgagat ttggacgcgg tcgttatcac
     3241 gcacgcgcac atcgaccaca cgggctatct gccgcgcatc gtccggctcg gctacgacaa
     3301 aaaggttttt acgtccgacg gaaccgccga tttgctaaaa attctgctgc ccgattccgg
     3361 cagattgcag gaagaagacg ccgaataccg caaccgcaaa ggcttgaccg tgcatcgacc
     3421 ggcgctgccg ctttatgacg aaacggacgc gaaagacgct ctgaaacttc tgaatcccgt
     3481 tccgaacaac ggcgagccgg tcgaaatttg cgaaggcttc agcgccgcgt tttacgtcgc
     3541 cggacacatt atgggcgcaa gcctcgtact gattgagatt gcgggcgcga aagagaacgg
     3601 cgagccgctc aggtttttgt tttccggcga tttgggacat tacgaccagc cgattatcaa
     3661 agacccggcg attccggtcg aatgcgatta tttgatggtc gaatcgactt acggcaaccg
     3721 cctgcacggc gacgtcgctt ccgacaccca actggcagag attatcaacg aagcgtttcg
     3781 acgcaacgcg ccgattttga ttccggcttt tgccgtcggg cgcacgcagg agcttttgta
     3841 tctgattcgc gaattggaag acgacggaaa aattccgatt ctgccggtcg tcgtcgattc
     3901 gccgatggcg gcgcaggcga cgcaggttta caatcgctgg cacgacgagc acgacgagga
     3961 atacgcttcg attctgatgc aaaagcgaca tccgctgcgc accggctcga tgacgaccgc
     4021 ttcgacgcgc gaagactcga agcgcctgaa cgatatgaaa ggcgcgcgca tcatcatctc
     4081 ggcttcggga atgctgacgg gcggtcgcgt cctgcatcac gcgatgagaa ttttaccgaa
     4141 cgaaaacgcg acgattgttt tcgtcggtta ccaggcggcg ggaacgatcg gcagacgtat
     4201 cca
//
LOCUS       DASWNE010000035         2140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_284001, whole genome shotgun sequence.
ACCESSION   DASWNE010000035 DASWNE010000000
VERSION     DASWNE010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284001"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="VGB68_00740"
     CDS             complement(<1..883)
                     /locus_tag="VGB68_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY0047781.1"
                     /translation="MQGISKKENLQLPRRLRGVAPTLIRQIFERALPDSINFGLGEPD
                     LPTPRFMREEAARVTLEEQNGYTSHAGLLPLREKIAEQYPHLNLDAKTQICVTVGSQE
                     AMTAAFLGIVDEGDEVLIPNPSFPAYENCVKISGGEPVFYRLPADKDFAFDIEEFKSK
                     ITPKTKAAVVISPSNPTGKIFTENDLKQIAGVLQETGIYLISDEIYSDLYFTEKPRSA
                     SEFYDKTVIVSGLSKSLSMTGWRVGWVASSQADVVKAALTLHGFLTVCTSTISQKASL
                     LGWTAEAERAKAEAREIY"
     gene            complement(890..1795)
                     /locus_tag="VGB68_00745"
     CDS             complement(890..1795)
                     /locus_tag="VGB68_00745"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0047782.1"
                     /translation="MYKIFISILLIVLSVFSGFAQENLNSAQLNLIAADKSNNPIGDL
                     GKDEITLLIDGKPQPVTSLEKQKAPLIYALALDSSGSMKSVFRDIQHVAVSIIAQNRA
                     NDETTLVSFIGSSQIYAPLGFSSDKNLLTRAINEFAVEGGQSAIIDAVYLSVKKVGEY
                     KREDKTRPRAVIIITDGEDRDSYYTEKALFKLIENETVPVFFIGLVNGLDDSTQFAPR
                     SRKERAENFIKKVSEKSGGTAIFPKKLTDAADRITSLLQMQYVVGYSPSAAVKGKSSK
                     IEIKLAKDSKRKDVKFYVNSNNTEK"
     gene            complement(1892..>2140)
                     /locus_tag="VGB68_00750"
     CDS             complement(1892..>2140)
                     /locus_tag="VGB68_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047783.1"
                     /translation="SHISHTAERYKLDSGRSALVIQAGTATSTRGRGEANSFNVIEFE
                     QPLLTVRRLECEKPETGFVLAQTQKFLQTEKGWTPEAA"
BASE COUNT          477 a          554 c          508 g          601 t
ORIGIN      
        1 ggtaaatttc gcgcgcttcg gctttcgccc gctcggcttc cgcagtccag cctaaaagcg
       61 aggctttctg ggaaatcgtc gaggtgcaaa ccgtcagaaa tccgtgcaga gtcaaagcgg
      121 ctttgacgac gtccgcttgc gagctggcga cccagccgac gcgccagccc gtcatactca
      181 gagattttga aagtccgctg acaatcacgg ttttatcgta aaattccgaa gccgagcgcg
      241 gtttttcggt aaaatacaag tccgaataaa tctcgtcgga aatcaaataa attcccgttt
      301 cctgcaaaac gccggcaatc tgttttaaat cgttttcggt aaagattttt ccggtcgggt
      361 tcgacggcga aataacgacc gcggctttcg ttttcggcgt gattttcgat ttgaattctt
      421 cgatgtcgaa ggcaaaatcc ttatccgccg gcaggcgata aaacacgggc tcgccgcccg
      481 aaattttcac gcagttttca tacgccggaa acgacggatt cggaatcaaa acctcgtcgc
      541 cttcgtccac gattcccaaa aatgcggcgg tcatcgcttc ctgcgagccg accgtcacac
      601 aaatttgcgt cttcgcgtcc aaattcaaat gcggatactg ctcggcgatt ttttcgcgca
      661 gcggaagcag tccggcgtgc gaagtgtagc cgttctgctc ttccagcgtg acgcgcgccg
      721 cttcctcgcg cataaaccgc ggcgtcggca aatccggctc gcccagaccg aaattaatcg
      781 aatcgggcag agcgcgctca aaaatctgtc gaatcaaagt cggagcaacg cctcgcagac
      841 ggcgcggcag ttgtaaattt tcctttttcg atattccctg catctttttt tatttttccg
      901 tgttatttga attaacgtaa aattttacgt cttttcgttt tgaatctttg gctaatttta
      961 tctcaatttt ggaagatttg cctttgaccg ccgccgaagg cgaatagccg acgacgtatt
     1021 gcatctgcaa aagggaagtt atccggtcgg cagcgtccgt taattttttc ggaaaaatcg
     1081 ccgtgccgcc gcttttctcc gaaacttttt tgataaaatt ttctgctctt tcctttctgc
     1141 ttcgcggcgc aaattgggtg ctgtcgtcta atccgttgac cagaccgata aagaaaaccg
     1201 gcactgtctc gttttcgatt aatttaaaaa gcgccttttc agtgtagtaa ctgtcgcggt
     1261 cttcgccgtc ggttattata atcacggcgc gcgggcgcgt tttatcttcc cttttgtatt
     1321 cgccgacttt tttgaccgac aggtaaacgg cgtcgataat ggcgctctga ccgccttcga
     1381 ccgcaaactc gtttatcgct ctggttaata aatttttatc ggagctgaat ccgagcggcg
     1441 cgtatatctg gctgctgccg ataaagctga cgagcgtcgt ttcatcattc gcccggtttt
     1501 gagcgattat ggagacggca acgtgttgaa tatcgcgaaa aaccgacttc atactgcccg
     1561 aactgtccag cgcgagcgcg taaatcaagg gcgctttttg tttttccagc gaggttaccg
     1621 gctgcggttt gccgtcaatc aataaagtta tttcatcttt gccgaggtcg ccgattggat
     1681 tgttcgattt gtccgccgct attaaattta gctgtgccga attcagattt tcctgcgcaa
     1741 atccggaaaa tacggagagg acgattaata aaattgaaat aaatatttta tacatatcct
     1801 ttcgaatctg cgctccgctt tttaaattaa cgctttcgct cgcgccgctc aaatgccgtt
     1861 tttgtctttc ggtaaagcgt ttgagctttc tttatgccgc ttcgggcgtc catccttttt
     1921 cagtttgcag aaatttctga gtctgagcca gaacaaagcc cgtctccggc ttttcgcatt
     1981 ccagacgccg gacggtcaaa agcggctgtt caaattcgat cacattaaaa gaattggctt
     2041 cgccgcgccc gcgggtcgag gtcgccgtgc cagcctgaat gacgagcgcg ctgcgtcccg
     2101 aatccaattt ataacgctca gccgtgtgcg agatatggct
//
LOCUS       DASWNE010000036        11572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_601969, whole genome shotgun sequence.
ACCESSION   DASWNE010000036 DASWNE010000000
VERSION     DASWNE010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11572
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601969"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VGB68_00755"
     CDS             <1..244
                     /locus_tag="VGB68_00755"
                     /inference="COORDINATES: protein motif:HMM:NF020034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0047784.1"
                     /translation="LMIDARHQGKGYGKAATLEVIRRMSEIEDCRAIYLSFVPENTGA
                     EKLYSSVGFERTGALNGNEIVMRFTIENNANLKSPI"
     gene            262..1074
                     /gene="lgt"
                     /locus_tag="VGB68_00760"
     CDS             262..1074
                     /gene="lgt"
                     /locus_tag="VGB68_00760"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEY0047785.1"
                     /translation="MYPELFRIGDFPINTYGLLLAVGMMLALFVAARLGARDGLPRER
                     MYDLGLWTLIGGLLGSKLLMLFVDDTYRNNIGNIFSFDFLRSGGVYYGGFLGGFFTVF
                     LLIRWYKLSFWKVADAFAPGLALGQFFGREGCFAAGCCWGKPTDLPWGVHFTEQANEF
                     TGVPIYGEGNSPLYLHPTQLYESITMLIVFGLLLYLHRHKRFDGQVLIAYAIIYALVR
                     FSIEFVRDDPRGDLFGLTTLTGLSTSQIISLMVAAGAVIFMIVRLRRTPETV"
     gene            1435..2403
                     /locus_tag="VGB68_00765"
     CDS             1435..2403
                     /locus_tag="VGB68_00765"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY0047786.1"
                     /translation="MNIPETNENILNFAPEAHDAGKRLDAFLAEKIENWSRSRLQRLI
                     DDGDVSVNEKQVKSSYKIRSNDEIEVELTELPVAAFEPENIPLEIVYEDEFLAVINKP
                     AGMVVHPGAGISGGTLANAIAYHFNPERSAEGNQPPGFRVGIVHRLDKDTSGLIVVAK
                     NQEAHDALSEQFRNREVFKSYLALAHGEIEETSGTIDAPIAREKHNRTKMSVRAHGRN
                     ALSLWKVRQRFEKFTLLGVEIKTGRTHQIRVHLAYINHPVVGDETYNSGRDKTVKDLE
                     IRRAIADLNRFFLHAEKLSFTHPQTKEKINFTAPLAPELNAFLETL"
     gene            2510..5527
                     /locus_tag="VGB68_00770"
     CDS             2510..5527
                     /locus_tag="VGB68_00770"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEY0047787.1"
                     /translation="MKKPISVLLPGLVFLVFLSVNLFAQTRDYTRYVNPFVGTGGHGH
                     TFPGATVPFGFVQLSPDTRMDDWDGSSGYHYSDNQIYGFSHTHLSGTGIPDYCDVLFA
                     PTVGEPEFFARRGDLSENGYASRFSHQNEKAEPGYYSVKLDEGNILAEMTATARVGFH
                     RYTFPAGTDANLILDLKHRDKVLDSELRVTGANRIEGFRRSSSWAKDQIVYFTAEFSE
                     PFTDYGVASDDGAPEKTAKSKGTNIKAFFRFAAKKPLLVKVAISPVGISGVRKNLLTE
                     IPHWDFEKVRRDAQGVWNKELSKIEVSGGSDEQLTNFYTALYHTMIHPNVFQDVDKSY
                     VGLDKQVHSARDFTNYTVFSLWDTFRAAHPLYTIIDQKRTADFINTFLKQYEQGGRLP
                     VWELAANETDTMIGYHAVSVIADAMAKDIEGFDYEKAFEAAKHSAELNHFGLEAYKKR
                     GYISMEDEQESVSKTLEYAYNDYCIARMAMSLMVKGSGEIKARTADERKQYQEKRKKL
                     DDDFWKYSQRAKYFENLFDAETGFMRPKQNGGFVKPFAPNEVTFHFTEGNSWQYSFFV
                     PHDINRLIALHRGSDRFAEKLDELFTTDSSLAGREQPDITGLIGQYAHGNEPSHHIAY
                     LYNYARQEWKTQMYVRRIMDEFYKPTPDGLIGNEDCGQMSAWYVLSASGFYPVTPGDA
                     SYDIGTPLFKEVKYNLENGKTFTIKAPNASGENIYVKSLKLNGKTHQTPLLRHGYIMN
                     GGVLEFEMSPVPVKDFFLGFSKSGIDTRIPAVPLIEGTEKVFKNQNTIALKTTAPKTR
                     IFYTLDGSDPDAKSKLYEKPFTIDKTATVKAVSVFPKGGRSPVAEARFYKKPNDWTVQ
                     LFSKYNRQYTGGGDEGLIDGIRGTSNFASGEWQGFQAQDFVAVIDLQKAAEIRKLGGG
                     FLQVARSWIWMPTRVEFEVSSDNVNFTKVADIKTDISPEDMEHKIRDYKTEIKPVKAR
                     YVRVKAYNPGKIPAWHPGAGFDAFIFVDEIFIE"
     gene            5670..7205
                     /locus_tag="VGB68_00775"
     CDS             5670..7205
                     /locus_tag="VGB68_00775"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY0047788.1"
                     /translation="MKKKLLLFFVLFVFPAFVLAQDYSAKLAEIDAYAQKVQTDWNVP
                     GLAVGIVKDDKVIFAKGYGVRQLGKTERVDENTLFAIASNSKAFTTASLAILIDEGKL
                     KWDDKVSQYLPEFQMFDPYVTRELTIRDLVTHRSGLDTFSGDLLWYETNYSTDEILRR
                     VRYLRPTSSFRSRYGYQNLMFTAAGRIVEKVSGKSWGDFVRERILTPLGMTRTTASVK
                     DLKDNYSMPHNESGGSLRVLHVGNVDAAAAAAGLNSSVREIAAWLRLQLGRGTFEGKK
                     IFSRERSAEMWSAVTPLGVAPFPAENAPTRLFSAVGMGWFLNDYRGRKMVSHTGGLDG
                     MLSQTAMLPEENLGLVVLTNSEAGAYSPIVNKILDVFLEAAPKRDWSTERLERIKQNK
                     TREAEEDKKLIAARVPNTKPSLALADYAATYSGEMYGDATVTTENGKLVLRLIPAPNF
                     VADLEHWYYDTFLIKWRPTVAYNFPRGFVSFTIDKTGRANELKIDQPNNDFWFYELEF
                     KRK"
     gene            complement(7336..8664)
                     /locus_tag="VGB68_00780"
     CDS             complement(7336..8664)
                     /locus_tag="VGB68_00780"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HEY0047789.1"
                     /translation="MSLIETIQSKAVRLNDARDVNDRILDLIGDARFVLIGEASHGTH
                     EFYQCRASITKRLIAEKGFAAVAVEADFPDAYRVNRFVRGIGDDENADQSLGDFDRFP
                     LWMWRNTEVLDFVEWLKEFNADKPLTNQAGFYGIDLYSLHASMEAVLEYLDKIDPEAA
                     RRARSRYSCFDHHGEDAQSYGYAASFDLSQSCEDEAVNQLVELQRRAAEYANRDGFVA
                     RDEYFFAEQNARLVKNAEEYYRQMFRGRVSSWNLRDSHMAETLSALATHLENQGQSAK
                     IVVWAHNSHLGDARATDMSRGRGEWNVGQLVREKYGAGDVRIIGFTTFEGTVTATTNW
                     DEPAQLKRVKQGLNNSYERLLHQTGIQNFFLDLRDEETKEILRRTMLERAIGVIYRPE
                     TERVSHYFEASLSEQFDGVIHFDRTRALEPLDKVASWRHDDAPETFPEGL"
     gene            complement(8726..8920)
                     /locus_tag="VGB68_00785"
     CDS             complement(8726..8920)
                     /locus_tag="VGB68_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047790.1"
                     /translation="MSQTQKAPDEETERPELEVNEKTQTPFSRQAGYNQPLPDGEGET
                     GGRAKRQTGSTLEVKPINPT"
     gene            complement(8950..9210)
                     /locus_tag="VGB68_00790"
     CDS             complement(8950..9210)
                     /locus_tag="VGB68_00790"
                     /inference="COORDINATES: protein motif:HMM:NF042641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6582 domain-containing protein"
                     /protein_id="HEY0047791.1"
                     /translation="MSNQNSKIDKRDDVNPDEGKDKYGDVEFADTTNNKYPIDTPERV
                     RAAWSYINQKDNAAKYDADEVQTIKSRIKKAADKHGVEISED"
     gene            complement(9262..9534)
                     /locus_tag="VGB68_00795"
     CDS             complement(9262..9534)
                     /locus_tag="VGB68_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047792.1"
                     /translation="MARCEVCGNDYYLSFEVITAGNRHVFDCFECAIHKLAPVCSHCG
                     CKIIGHGIEAKETFFCCANCARQAGVKGVEDHIWGKEKETAERLAE"
     gene            complement(9770..11446)
                     /locus_tag="VGB68_00800"
     CDS             complement(9770..11446)
                     /locus_tag="VGB68_00800"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0047793.1"
                     /translation="MIFRFLRTAQLQSLFALMLLFALVSQVTAQQTAPALLSQTEKTL
                     AANIKIQTIKEITAALAADEMQGRGTMQPGGDKAASWIADRFQKLGLKPLGDKGSYLQ
                     KIEFKETILAPETSLKVGVENLKQGSDYTFLPLPFTPVDKSAGGEMVFIAYGIQSESI
                     KRDDLKDIDVRGKIVVMMDGPPANMSQEVWDKTNVKMTGFVNLVRNGAAGIIFIAHGR
                     EKEPLETYIDYLGRRQIAMADKEGDSEPIPLPPLVLAGGKAAEKIFAKSGMSLKDALA
                     RAEENSFKPFSLNQEAKIVSKYKTTRGVSSNVAGYIEGSDPKLKEEAVLFSAHYDAYG
                     MENGKIYHGAADNALGVAEMLAVAEAYTKLPAKPKRSMVFLAVTGEEYGLFGSKYWAK
                     NPTWNIKKVAANLNLDGIGTEVYAPVKTFVGFGAEHSTIGAILTDVSGAFGIKVIPDP
                     MPDEKIFYRSDHYSFVERGVPALMLLGAPEGDPQAWIKRSKDWEKTDYHQPSDKIRPD
                     WSWEGAKTVADVMGIMGWRISETEKMPEWLPSSRFGKLERGNTKELSEEK"
BASE COUNT         2736 a         2996 c         2937 g         2903 t
ORIGIN      
        1 cctgatgatc gacgcgcgcc atcagggcaa aggctacggc aaagcggcga cgctggaagt
       61 tatccgtcga atgagcgaaa tcgaagattg ccgggcgatt tatctgagct ttgtgcccga
      121 aaatacgggc gcggaaaagc tttattcgag cgtcggcttt gaacgcacgg gcgcgcttaa
      181 cggaaacgag attgttatgc gatttacgat agaaaacaac gcaaatctaa aatcgccaat
      241 ctaaaatcgc aaatccatat tatgtatccg gaactttttc gaatcggcga tttcccaatc
      301 aatacttacg gcttgctgct ggcggtcggg atgatgctcg cgcttttcgt cgccgcgcgt
      361 ttgggcgcgc gcgacggtct gccgcgcgag cgaatgtatg atttgggttt gtggacgctg
      421 atcggcggtc tgctcggctc gaaactgctg atgctgtttg tcgacgacac ttatcgaaac
      481 aacatcggaa acattttctc attcgatttt ctgcgttcgg gcggcgttta ttacggcggt
      541 tttttaggcg gattttttac ggttttcctt ttgattcgct ggtataagct ctcgttctgg
      601 aaagtcgccg acgcgtttgc gcccgggctc gcgctcggac aatttttcgg gcgcgaaggc
      661 tgtttcgccg ccggatgctg ctggggaaaa ccgacggatt tgccgtgggg cgtgcatttt
      721 accgagcagg cgaacgaatt tacgggcgtt ccgatttacg gcgaaggcaa cagcccgctt
      781 tacctgcacc cgacgcagct ttacgaatca atcacgatgc tgattgtttt cggcttgctg
      841 ctttatctgc atcgacacaa gcgttttgac gggcaggttt tgattgccta cgcgattatt
      901 tacgcgctgg tgcgtttttc cattgaattt gtccgcgacg acccgcgcgg cgatttgttc
      961 ggattaacca ctttgacagg tctttcgacc tcgcagatta tcagcctgat ggtcgcggcg
     1021 ggcgcggtga tttttatgat tgtcagattg cgccggacgc cggaaacggt ttagcgtcaa
     1081 tcgaaattat tcaatttccc ttaaaggtta tttaattttc ccgtgagatt attgaatttc
     1141 ccttaatggt aattgagttt cccttaatgg taattcaatt tcctttagtg gtaattgagt
     1201 ttcccttaga gattattcaa tttcccttaa aggttattca atttccctta aaggttattc
     1261 aatttccact gagggcaatt gagtttccct taaaggtaat tgactttctc ttagaggtaa
     1321 ttgactttct cttagaggta attgagtttc ctttagagat tatttaatca tctcacgata
     1381 taatcgaata atcattaaag atttcagagc agctttcaaa aattatcttc cagcgtgaat
     1441 attcctgaaa caaacgaaaa cattctcaac tttgcgccgg aagcgcacga cgccggaaaa
     1501 cggctcgacg cttttctcgc cgaaaaaatc gaaaactggt cgcgctcgcg cctgcagcga
     1561 ttgattgacg acggcgacgt ttcggtcaac gaaaaacagg taaaatcctc atacaaaatc
     1621 cgctctaacg acgaaatcga agtcgagctg acggagcttc ccgtcgccgc gttcgagccg
     1681 gaaaatattc cgctcgaaat cgtctacgaa gacgaatttc tcgccgtcat taacaaaccc
     1741 gccggaatgg tcgtgcatcc gggcgcgggc atctccggcg gaacgctggc aaacgcgatt
     1801 gcttatcatt tcaatccgga gcggtcagcc gagggcaatc agccgccggg ttttcgcgtc
     1861 ggcatcgttc atcgccttga taaagacacg tccggcttga ttgtcgtggc taaaaatcag
     1921 gaggcgcacg acgctctgag cgagcagttt cgcaaccgcg aggtttttaa aagctatctg
     1981 gcgctggcgc acggcgaaat cgaggaaacc agcggaacga ttgacgcgcc gatcgcgcgc
     2041 gagaaacaca atcgaaccaa aatgtcggtg cgcgctcacg gtcgcaacgc gctgtcgctc
     2101 tggaaagtgc ggcagagatt tgaaaaattc acgctgctcg gcgtagaaat caaaaccggc
     2161 agaacgcatc aaatccgcgt tcacctcgcc tatataaatc atccggtcgt cggcgacgaa
     2221 acttataatt ccgggcgcga caaaaccgtc aaggatttgg aaatccgccg cgccatcgcc
     2281 gatttaaacc gcttttttct gcacgccgaa aaattatctt tcacgcatcc gcagacaaag
     2341 gaaaaaataa atttcaccgc gccgctcgcg ccggaattaa acgcgtttct ggaaaccctg
     2401 taaaaataaa gcttagtcga gcaaattatc ctcgatttaa ttgacaccgc ttctcaaact
     2461 gttttagaac ttgataaaag catttcattt tcgcttcaga atctccgata tgaaaaaacc
     2521 gatttcagtc ttgctgccgg gtcttgtttt ccttgttttt ctttccgtta atcttttcgc
     2581 ccagaccaga gattacacgc gctacgtcaa tcctttcgtc ggcacgggcg gtcacgggca
     2641 cacgtttccg ggcgcgaccg ttcccttcgg cttcgtccag ctttcgcccg acacgcggat
     2701 ggacgattgg gacggaagca gcggctatca ttattccgac aaccagattt acggcttttc
     2761 gcacacacac ctgagcggca cgggcattcc cgattactgc gacgttttgt ttgcgccgac
     2821 ggtcggcgag ccggaatttt tcgcgcgccg gggcgatttg agcgagaacg gctacgcttc
     2881 ccgtttttcg catcaaaacg aaaaagccga gccgggttat tattcggtaa aactcgacga
     2941 aggaaatatt ctggcggaaa tgaccgcgac cgcgcgcgtc ggttttcacc gctacacttt
     3001 tccggcgggc actgatgcga atttgattct ggatttgaag caccgcgata aagttctgga
     3061 ttcggaactg agggtcacgg gcgctaaccg catcgaaggt tttcgccgtt cgagctcgtg
     3121 ggcgaaagac caaatcgttt atttcaccgc cgaattttcc gagccgttca ccgattacgg
     3181 cgtcgcatcg gacgacggcg cgcccgaaaa aaccgccaaa tcaaaaggca cgaacatcaa
     3241 ggcttttttc cgtttcgccg ccaaaaaacc gttgctcgtc aaagtcgcca tttcgccggt
     3301 cggcatcagc ggcgtgcgca aaaatctgct gaccgagatt ccgcactggg attttgaaaa
     3361 agtgcgacgc gacgcgcaag gcgtctggaa caaagaactc tcgaaaatcg aagtttcggg
     3421 cggcagcgac gaacaattaa ccaatttcta caccgcgctt tatcacacga tgattcatcc
     3481 gaacgttttt caggacgtcg ataaaagcta tgtcgggctg gataaacaag ttcattcggc
     3541 gcgggatttt accaattaca cggttttttc gctttgggac acgtttcgcg ccgcgcaccc
     3601 gctttacacg attatcgacc aaaagcgcac ggcggatttt atcaacacct ttctgaaaca
     3661 atacgagcag ggcgggcgct tgcccgtctg ggaattagcc gccaacgaga ccgacacgat
     3721 gatcggctat cacgccgttt cggtgattgc cgacgcgatg gcgaaagaca tcgaaggctt
     3781 tgactacgag aaagcgttcg aggcggcgaa acattccgcc gaactgaatc atttcggact
     3841 cgaagcgtat aaaaaacgcg gctatatctc gatggaagac gagcaggaat ccgtctccaa
     3901 aactctggaa tacgcctaca acgattattg cattgcgcga atggcgatga gtttaatggt
     3961 taaagggtcg ggagaaatta aagccagaac cgccgacgag cgaaagcaat atcaagagaa
     4021 aaggaaaaaa ctggatgacg atttttggaa atacagccag cgcgcgaaat attttgaaaa
     4081 tctttttgac gcggaaaccg gctttatgcg cccgaaacaa aacggcggtt tcgtcaaacc
     4141 ttttgcgccg aatgaagtga cgtttcattt taccgaggga aattcgtggc agtattcgtt
     4201 tttcgtgccg cacgatataa atcgtttaat cgcgctgcat cgcggctcag acaggttcgc
     4261 cgaaaaactg gacgagcttt ttacgaccga ttcgagcctc gcgggacgcg aacagcccga
     4321 cattaccggc ttaatcggac aatacgctca cggcaacgag ccttcgcatc acatcgcata
     4381 tctttacaat tacgcgcggc aagagtggaa aacgcagatg tacgtccgca ggattatgga
     4441 cgagttttac aagccgacgc ccgacggtct tatcggcaac gaagattgcg gacagatgtc
     4501 ggcgtggtat gttttgagcg cgtccggctt ttatccggtt acgcccggcg acgcttctta
     4561 tgacatcggc acgccgcttt tcaaagaagt aaaatacaat ctcgaaaacg gcaagacgtt
     4621 tacgattaaa gcgccgaacg cgtcgggcga aaatatctat gtcaaatcac tcaagctaaa
     4681 cggcaaaacg catcaaacgc cgcttctccg gcacggatac attatgaacg gcggcgttct
     4741 ggaatttgaa atgtcgccgg tgccggtaaa agactttttt ctcggttttt ccaaatccgg
     4801 catcgatacg agaatacccg ccgttccgct gatagaaggg acggaaaagg ttttcaaaaa
     4861 tcaaaataca atcgctctga aaacgactgc gccgaagaca cgaatttttt atactctgga
     4921 cggaagcgac ccggacgcga aatcaaagct ttacgaaaaa ccttttacga ttgataaaac
     4981 cgcgaccgtt aaagccgttt ccgttttccc gaaaggcgga agatcgccgg ttgccgaagc
     5041 gcgcttttac aaaaagccga acgattggac ggttcagtta ttttcaaaat acaaccggca
     5101 atacacgggc ggcggcgatg aaggcttgat tgacggcatt cgcggcacgt cgaatttcgc
     5161 atcgggcgaa tggcagggct ttcaggcgca ggatttcgtc gcggtcatcg atttacaaaa
     5221 agcggcggaa atccgaaagc tgggcggcgg atttttgcag gtcgcgcgaa gctggatttg
     5281 gatgccgacg cgcgtcgaat ttgaagtttc aagcgataac gtaaatttca cgaaagtcgc
     5341 cgacatcaaa acggacatct cgcccgaaga tatggagcac aaaatccgcg attacaaaac
     5401 cgaaatcaag ccggtcaaag cgcgctacgt gcgcgtcaaa gcttacaatc ccggcaaaat
     5461 tccggcgtgg catccgggcg cgggatttga tgcgtttatc ttcgtggacg agatttttat
     5521 cgagtaagat tattcggaaa aatcttctcg tcaacttcag gctgattttt caaatgacga
     5581 aaacgtgaaa ctcgaaatgg cgggctgttt tcgttgttga ccttttatgt taaacttctc
     5641 gaaaattctc atctgaaaag gtttcaatta tgaaaaaaaa attactgttg ttttttgttt
     5701 tattcgtttt cccagccttt gttctcgcgc aggattacag cgcaaagctg gcggaaatcg
     5761 acgcctacgc gcaaaaggtt caaaccgact ggaacgttcc cggtctagcc gtcggaatcg
     5821 tcaaggacga taaagtaatt ttcgccaaag gctacggcgt gcgccagctc ggcaagaccg
     5881 aaagagtcga cgaaaacact ttgtttgcca tcgcttcaaa ctcgaaggct tttacgaccg
     5941 cgtccttagc gattttgatt gacgaaggca agctgaaatg ggacgacaag gtatcgcaat
     6001 acctgcccga atttcagatg tttgacccgt atgtgacgcg cgagctgacc atccgcgatt
     6061 tggtcacgca ccggagcggg ctggatactt tcagcggcga cctgctctgg tatgaaacga
     6121 attattcgac cgacgagatt ctgcgccgcg tgcgctatct gagaccgact tcgagctttc
     6181 gcagccgcta cggttatcag aatctgatgt ttacggcggc gggcagaatc gtcgaaaaag
     6241 tttcgggcaa aagctggggc gatttcgtgc gcgaaagaat tttaacgccg ctcggaatga
     6301 cgcggacgac cgcgagcgtc aaagatttga aagataatta ttcgatgccg cacaacgaat
     6361 cgggcggcag cctgcgcgtt ttgcacgtcg gaaacgtgga cgcggcggcg gcggcggcgg
     6421 gcttgaattc cagcgtcaga gaaattgccg cctggcttcg cctgcaattg gggcgcggca
     6481 cattcgaagg caaaaagatt ttcagccgcg agcgctcagc cgaaatgtgg tcggcggtga
     6541 cgccgctcgg cgtggctcct tttcccgccg agaacgcgcc gaccagactt ttttcggcgg
     6601 tcggaatggg ctggtttctg aacgattatc gcggcagaaa aatggtttcg cacacgggcg
     6661 ggctggacgg aatgctttcg cagacggcga tgctgcccga agaaaatctc ggtctggtcg
     6721 tgctgaccaa ttccgaagcg ggcgcttact cgccgatagt caacaagatt ctggatgttt
     6781 ttctcgaagc cgcgccgaaa cgcgattgga gcaccgaacg cctcgaaaga attaaacaaa
     6841 acaagacgcg cgaagccgaa gaagataaaa aactaatcgc cgcgcgcgtg ccgaacacga
     6901 agccttcgct ggcgctcgcc gattacgccg caacctacag cggcgagatg tacggcgatg
     6961 cgaccgtcac gacggaaaac ggcaaattgg tgctgcgcct catccccgcg ccgaatttcg
     7021 tggcggattt ggaacactgg tattacgaca cttttttaat caagtggcga ccgaccgtcg
     7081 cgtataattt cccgcgcggc ttcgtcagct ttacgattga taaaaccggc agggcgaacg
     7141 aattaaaaat cgaccagccg aataacgatt tctggtttta cgagctggaa ttcaagagaa
     7201 aatgatgaac gcgcaacgat gaacgcgaaa ttaattatga attatgaatt atgaattaat
     7261 gatttccggc aacttaaaat cataattcaa aactatcttt tgttcatcat tcatcgttcc
     7321 gcgttcatcg tttctttaca atccttcggg aaaggtttcg ggcgcgtcgt cgtgtcgcca
     7381 gctcgcaact ttgtcgagcg gttcgagcgc gcgcgtccgg tcgaaatgaa ttacgccgtc
     7441 gaattgttcg gacagactcg cttcgaaata atgactgacg cgctcggtct caggtcggta
     7501 aatcacgccg atcgctcttt ccagcatcgt tcggcgcaaa atttctttcg tttcttcgtc
     7561 gcgtaaatcc agaaagaaat tttgaatgcc ggtttgatgc aaaagacgct cgtagctgtt
     7621 attcaagccc tgtttgacgc gcttgagctg cgccggctcg tcccagttgg tcgttgccgt
     7681 gaccgtgcct tcaaaggtcg tgaaaccgat tatgcgcaca tcgcccgcgc cgtatttttc
     7741 gcgcaccagc tgaccgacat tccactcgcc gcgtccacgg ctcatatcgg tcgcgcgcgc
     7801 gtcgccgaga tgcgagttat gcgcccagac gacgattttc gcgctctgcc cctgattttc
     7861 cagatgcgtc gccagagcgg atagagtttc cgccatatga ctgtcgcgca gattccagct
     7921 ggaaacgcgc ccgcgaaaca tctgtcgata atattcttcg gcgtttttca caaggcgcgc
     7981 gttctgttcg gcgaaaaaat attcgtcgcg cgcgacgaat ccgtcgcgat tcgcgtattc
     8041 ggcggcgcgg cgctgcagtt cgaccagttg attgacggct tcgtcctcgc acgactgcga
     8101 caaatcgaag ctcgcggcat agccgtagct ttgcgcgtct tcgccgtggt ggtcgaaaca
     8161 ggaataacgc gaacgagcgc gccgcgccgc ttccgggtcg attttgtcca gatattccaa
     8221 aaccgcttcc atcgaagcgt gcaggctgta taaatcgatg ccgtaaaatc cggcttgatt
     8281 agtcagaggc ttatcggcgt taaattcctt cagccactcg acaaaatcca gaacctccgt
     8341 gttgcgccac atccagagcg gaaaacggtc gaaatcgccc agcgactggt cggcattttc
     8401 gtcgtcgccg atgccgcgga caaaacgatt aacgcggtaa gcgtccggga aatcggcttc
     8461 gacggcgacg gcggcaaaac ctttttccgc gattaatctt ttcgttatcg aggcgcggca
     8521 ttgataaaat tcgtgcgtgc cgtgcgaggc ttcgccgatt aaaacgaaac gcgcgtcgcc
     8581 gattaaatcg agaatccggt cgttaacgtc gcgcgcgtcg tttaatctga ctgctttcga
     8641 ttgaattgtt tcgattaagc tcatagcctt tatagcctta tttctattca agattcgcaa
     8701 acaagttcta aattcgcaaa caaatctaag tcggatttat cggcttcacc tccaatgtcg
     8761 agccggtttg ccgttttgct cgcccgccgg tttcgccttc gccgtccggc agcggctggt
     8821 tgtagcccgc ctgcctcgaa aacggcgttt gcgtcttctc gtttacctct aattccggtc
     8881 gctccgtctc ttcatccggc gctttctgcg tctgtgacat ttcaaatctc ctcgatgctt
     8941 tatccgccgc taatcttcgc tgatttccac gccgtgtttg tcagccgctt ttttgatacg
     9001 gcttttaatc gtctgcactt cgtcggcgtc atattttgcc gcgttgtcct tctgattgat
     9061 ataactccac gcggcgcgca cgcgctcggg cgtgtcaatc ggatatttat tgttcgtcgt
     9121 atcggcaaat tcgacgtcgc cgtacttgtc tttgccctcg tcgggattga catcgtctcg
     9181 tttatcaatt tttgaatttt gattactcat ttttttctcc cttccgtttc ataacgcctg
     9241 cctgtaaatt tcggcgcggt tttactcggc aagccgctcc gccgtttcct tctctttgcc
     9301 ccagatatgg tcttcgacgc ctttgacgcc cgcctgtctc gcgcagttgg cgcagcagaa
     9361 aaaagtctct ttcgcctcga tgccgtgacc gataatcttg cagccgcaat gcgagcagac
     9421 cggcgcgagc ttgtgaatcg cgcactcgaa acaatcaaaa acgtgacggt tgcccgccgt
     9481 gataacttca aacgacagat aataatcgtt gccgcaaacc tcgcatcgtg ccatttttta
     9541 cagtctcctt cgattcaatt tgttctgtaa tataatctta gccgttcaaa aatcgaattg
     9601 ccagtttttt ctgaatattt accgggcaaa ccgaaaatga atattgttaa aaagattgga
     9661 cgaagattat ttaaaattta ttacaaatct attcgcttcc cgtagagacg gaaaaaggcg
     9721 ggcaagcccg cctctccacc atcaaattag tttaacttta tccgattgtt tatttttctt
     9781 cggaaagttc tttcgtgttg ccgcgttcga gtttgccgaa acgcgacgac ggcagccatt
     9841 cgggcatttt ttccgtttcg gagatgcgcc agcccataat tcccattacg tcggcgacgg
     9901 ttttcgcgcc ttcccaagac caatcggggc gaatcttgtc cgacggctgg tgataatcgg
     9961 ttttttccca atctttcgaa cgcttaatcc aggcttgcgg gtcgccttcg ggcgcgccca
    10021 gaagcatcag cgccggaacg ccgcgctcga caaacgaata atggtcggaa cggtaaaaaa
    10081 tcttttcatc cggcatcggg tcgggaatga ccttgatgcc gaacgcgccg gaaacgtccg
    10141 tcagtatcgc gccgatggta gaatgctccg cgccgaagcc gacaaaagtt ttgaccggag
    10201 cgtaaacctc cgtgccgatg ccgtcgagat tcagattggc ggcgactttc ttaatgttcc
    10261 aggtgggatt tttcgcccag tatttcgagc cgaaaagacc gtattcttcg cccgtcacgg
    10321 cgaggaagac catcgagcgt ttcggcttcg ccggcagttt cgtgtaagct tcggcgaccg
    10381 ccagcatctc cgcgacgccg agcgcgttgt cagccgcgcc gtgataaatt ttaccgtttt
    10441 ccataccgta ggcgtcataa tgcgcgctga acaaaacggc ttcttccttc agcttcgggt
    10501 cggagccttc gatatagccg gcgacgtttg acgaaacgcc tctggtcgtt ttgtatttgg
    10561 agacgatttt cgcttcctgg ttcagactaa acggtttaaa gctgttttcc tcggcgcggg
    10621 cgagcgcgtc tttcaggctc attcccgatt tggcaaatat tttttcagcc gctttgccgc
    10681 ctgccagcac cagcggcggc agcggaatcg gttcggaatc gccttctttg tccgccatcg
    10741 cgatttgccg ccgtccgaga tagtcgatat acgtttcgag cggttctttt tctctgccgt
    10801 gcgcgataaa aattattccg gcggctccgt ttctcaccaa attgacaaag cccgtcattt
    10861 tgacattagt tttgtcccag acctcctggg acatattggc gggcggaccg tccatcataa
    10921 cgacgatttt tcccctgacg tcgatgtctt tcaggtcatc ccgcttgatt gattcggact
    10981 ggatgccgta agcgatgaaa accatctcgc cgcccgcgct tttatccacg ggcgtaaaag
    11041 gcagcggcag aaaggtgtaa tcgcttccct gtttcaaatt ttcgacgccg actttcagcg
    11101 aggtttccgg cgcgagaatc gtttctttaa attcgatttt ttgcaggtat gagcctttat
    11161 cgccgagcgg tttcaagccc agtttctgga aacggtcggc aatccagctc gcggctttgt
    11221 cgccgcccgg ctgcatcgtg ccgcgtccct gcatttcgtc ggcggcgagc gcggcggtga
    11281 tttctttaat cgtctgaatt ttaatattcg ccgccagagt cttctcggtt tgagaaagca
    11341 gggcgggcgc ggtttgctgc gccgtaactt gcgaaacaag cgcgaagagc agcattagag
    11401 cgaaaaggct ttgcagttgc gccgttcgca gaaaacgaaa aatcatattt tgtaaaactc
    11461 cgtaggttaa gaaaattttc tcaggacttt gatgcttctc aaacggattt gattgcacca
    11521 gttacgataa cacagagatt tttcggcgtg aaatgcggaa cgaaccgaaa ga
//
LOCUS       DASWNE010000037         2243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_248620, whole genome shotgun sequence.
ACCESSION   DASWNE010000037 DASWNE010000000
VERSION     DASWNE010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248620"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1541
                     /locus_tag="VGB68_00805"
     CDS             <1..1541
                     /locus_tag="VGB68_00805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY0047794.1"
                     /translation="VIQDSYVGGKKLNYNDLIKSSIDSMLHSLDPHSNYFDAKEFEQF
                     RTDQQSQYFGIGATIGDLRDGKGNLIATYIKATFDGAPAHRAGLRYGDKIVDVNGTSM
                     LGKPFSDVRSFLRGPRGTTAKITVERYGTGKRDTVEITRDAVPQPSIPEAYMIRPGVG
                     YIAMTGGFNQTTFNEFRQAMVELKRQGMENLVLDLRNNGGGLVNQAYRVASTFLNRGQ
                     TVFTQKGRLEDSYGSYPSDNGAPDQTPIVIMVNRNTASASEILAGALQDHDRALIVGE
                     NTFGKGLVQNPFILEYGSMLLLTIAKYETPSGRLIQRDYSNGNLYDYYTGGGTLRDEK
                     PADKPKGAETKTDTGRIVYGGGGIQPDEQVKPQTIPAERGQLQAKLIDPIFAFSLDLV
                     YGKTPGFETYKADKPITFNYDLKATDFPINETIYQAFKKYAMDKYKLTPAQIDREREF
                     VERTLRTEFVTAAYGSTTSFQVFNEYDTQLKRAIEVLPKAKQLATESARVNAQKAISN
                     NNNR"
     gene            complement(1808..1975)
                     /locus_tag="VGB68_00810"
     CDS             complement(1808..1975)
                     /locus_tag="VGB68_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047795.1"
                     /translation="MNRPPPYFPQNQLSPPDPNRYKKFKLMVGAAIAVLTLFLIGAGV
                     GVYYLIKWMAA"
     gene            complement(2051..>2243)
                     /locus_tag="VGB68_00815"
     CDS             complement(2051..>2243)
                     /locus_tag="VGB68_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047796.1"
                     /translation="IGPLTQRGVPSFAPWFDQRTYFNYHHTAADTFDKVNPEELAKVG
                     SVMAVLAFGLANLEQPLPR"
BASE COUNT          578 a          585 c          586 g          494 t
ORIGIN      
        1 ccgtcattca ggactcttac gtcggcggca aaaagctcaa ttacaacgac cttatcaaat
       61 cttcgattga ttcgatgctg cactcgctcg acccgcactc gaattatttc gacgccaaag
      121 agttcgagca gtttcgcacc gaccagcaat cgcagtattt cggtatcggc gcgaccatcg
      181 gcgatttgcg cgacggcaaa ggcaacctga ttgcgaccta tatcaaagcg acgtttgacg
      241 gcgctccggc acatcgggcg ggcttgcgtt acggcgacaa aatagtcgat gtcaacggca
      301 cttcgatgct cggcaaaccg ttttccgatg ttcgctcttt cctgcgcgga ccgcgcggca
      361 cgaccgcgaa aatcacggtc gagcgttacg ggacaggcaa gcgcgacacg gtggaaatca
      421 cgcgcgacgc cgttccgcag ccttcgattc ccgaagctta tatgattcgt ccgggcgtcg
      481 gctatatagc gatgaccggc ggttttaacc agacgacttt taacgaattc cggcaggcga
      541 tggtggaatt aaagcgtcag ggaatggaaa atctcgtcct cgatttgcgc aacaacggcg
      601 gcggtctggt caatcaggct tatcgcgtcg ccagcacttt cctgaatcgc gggcagacgg
      661 ttttcacgca gaaaggacgc ctcgaagaca gctacggctc gtatccttcg gacaacggag
      721 cgcccgacca gacgccgatc gtcattatgg tcaatcgcaa cacggcttcg gcttcggaaa
      781 ttctcgccgg agcgcttcag gaccacgacc gcgcattaat cgtcggcgaa aacactttcg
      841 gtaagggctt ggtgcaaaat ccgtttattc tcgaatacgg ctcgatgctg cttttgacga
      901 ttgccaaata cgagacgcct tcgggtcggc tgattcagcg cgattattcg aacggaaatc
      961 tttacgatta ctacaccggc ggcggaacct tgcgcgatga aaaaccggcg gacaagccga
     1021 aaggcgcgga aaccaaaacc gatacgggac gcatcgttta cggcggcggc ggaatccagc
     1081 cggacgagca ggtcaagccg cagacgattc cggcggagcg cgggcagctg caagccaagc
     1141 tgattgaccc gattttcgct ttctcgctcg atttggttta cggaaaaacg cccggattcg
     1201 aaacctataa agccgacaag ccgattacgt tcaactacga tttgaaagcg acggattttc
     1261 cgattaacga gacgatttat caggctttca aaaaatacgc gatggacaaa tacaaattaa
     1321 cgcccgccca gattgaccgc gagcgcgaat ttgtcgagcg cactctgcgt acggaattcg
     1381 tcacggcggc ttacggctcg accacttcct tccaggtttt caacgaatac gacacgcagc
     1441 tcaaacgcgc catcgaagtg ctgccgaaag ccaaacaact ggcgaccgaa agcgcgcgcg
     1501 tcaatgcgca gaaagcgatt tcaaataaca ataatcgtta atctgagaaa ttcaatttaa
     1561 ggaaaaggtg tgtttttatt aacacacctt ttttcttttt gtatagagcg tttcagcgtc
     1621 cttgtgtggc tcaaggcgcc acttatggaa agcgcatcaa atgaatttaa agggattgac
     1681 aacgaggcag ttcgtagaga ttctctgaga ggctgcaagc agcgatgaag ttcccctcgg
     1741 aaaccgtgca gaacatagtc gaatgatgta aattgtcgct ttcgcctggc gcaggctaat
     1801 tccgctgtta cgcagccatc cacttaatca aataataaac gccgacgccc gcgccgatta
     1861 aaaacagggt cagaacggcg attgccgcgc cgaccataag cttaaatttt ttataacggt
     1921 tcgggtcggg cggcgagagc tggttttgcg gaaaatacgg cggcggtctg ttcataatat
     1981 ttaagattta acatttaaca tttatcaaag caacagccgg taaatgttaa atgttaaatg
     2041 tccggtgaaa ttatctcgga agcggctgtt cgagattcgc cagaccgaaa gccagaacag
     2101 ccataaccga gccgactttc gcaagctctt ccggattgac tttatcgaaa gtgtcggcgg
     2161 cggtgtggtg ataattgaaa tatgttcgct ggtcgaacca cggcgcgaaa gacggaacgc
     2221 ctctctgcgt cagcggtccg atg
//
LOCUS       DASWNE010000038         4066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_602061, whole genome shotgun sequence.
ACCESSION   DASWNE010000038 DASWNE010000000
VERSION     DASWNE010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602061"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VGB68_00820"
     CDS             <1..685
                     /locus_tag="VGB68_00820"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEY0047797.1"
                     /translation="GLSGEVARKFLEDREGNIWVGSMNGLERFRYSPIAPVKLPDDFH
                     QLTLVAGENGEIWIASAGKKTILRVGKTNSPPFELSETRISSFFRDTSGDIWWGGHGG
                     VWVNYGDEGLIYFKDGVWSRRKPPEGLPNRGPSASFHDERGRLWLGYTENRVFVLDNG
                     QVKGYLNKDGIDVGRIKVIRGRGDNIWVGGELGLGFLKGDRFHTVSANGEQLETISGI
                     IETAAAICG"
     gene            861..1223
                     /locus_tag="VGB68_00825"
     CDS             861..1223
                     /locus_tag="VGB68_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047798.1"
                     /translation="MHKNKENREKFRWLIVNSQLLLLNYFTKDTEHQFRIYNLEFTIS
                     PFFIFVQSRKLWFATDSGLAQIDPANLTKNPLPPPPVIKSIQTDEKLYRLDQDLVFPA
                     GTLPQQAAFKRNSFSPNR"
     gene            2139..>4066
                     /locus_tag="VGB68_00830"
     CDS             2139..>4066
                     /locus_tag="VGB68_00830"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEY0047799.1"
                     /translation="MRILKNTLQAGLLGMCLLLGVAGSQAADLTVNTTADAGIGSLRQ
                     AIIDATINAAANTITFNVSTTDPGYNAAENRFTVNLSSSLPDIPLAPLTINNFQSQGI
                     TVKGNNSFRIFTLVNSAVVTMNNLTISNGFSSDGLGGGIFMGNSSTLTLNGSNVSNNT
                     VSGNGGGIYMRDSATLTLANSTISGNTAGNGGGIYINDSGTLNISTSTVSGNTANNGG
                     SGGGVFNGTSGTINAANSTFDDNSAANNGGGIYNTATITLNSNTVTGNRAAKGGGIYN
                     NFTATLNNNLVALNAATDGNDLLGRGSLGNAFNGSYNLVRNADGSEGLGASNQLGTTL
                     NPIDPRIGPLKNNGGATFTRALHNRSPAIDKGNSPSLITDQRGKARPYDNPLVANAPG
                     GNGADIGAFERTFTPTPFDYDGDGRADVSVFRPSDGTWYLQKSTDGFSAVQFGISTDK
                     LAAADFDGDGKTDIAVFRPSNGTWYLQQSTNGFAAVQFGASGDIAQPADFDGDGRAEL
                     AVFRPSSGTWFTLSLADNQFSGVQFGNNGDKPVAADYDGDTKADYAVFRPSDGVWYSL
                     RSQSGFTAVQFGIASDKPVAADYDGDGKSDQAVYRDGVWYQLRSSQGFAAVQFGTATD
                     LAAPADFDGDGKADLVVF"
BASE COUNT         1080 a         1029 c         1045 g          912 t
ORIGIN      
        1 cggtttgtcg ggcgaagtcg ccaggaaatt tctggaagat cgcgaaggga atatatgggt
       61 cgggtcaatg aatggtttag agcgttttcg ttacagtccg attgctccgg ttaagctgcc
      121 cgatgatttt catcagctga cgctggtcgc cggcgaaaac ggagaaattt ggatagccag
      181 cgccggaaaa aaaacaattc tgcgagtcgg gaaaaccaat tcgccgccgt ttgaactttc
      241 cgaaacccgt atttcatcat tttttcgaga cactagcggt gacatctggt ggggcggaca
      301 cggcggagtc tgggttaatt acggcgatga agggttgatt tatttcaaag acggggtctg
      361 gtcgcggcgg aagccgcccg agggactgcc gaatcgcggt ccgagcgcat cttttcacga
      421 cgaacgagga cgcctctggc tcggttatac ggaaaacaga gtttttgttt tggataacgg
      481 tcaggttaaa gggtatttga ataaagacgg aattgacgtc ggcaggataa aagtcattcg
      541 cggtcgcggt gataatatct gggtcggcgg ggagcttgga ctcggttttc tgaaaggcga
      601 cagatttcac acggtgagcg caaacggtga acaactggaa acgatttccg gaattatcga
      661 aaccgcagcg gcgatttgtg gctgaacaca aataagggga ttttcagtat ttcatcgtcc
      721 gaaattgccc ggttaaaaga taaccctgat tataaagtga attatcgctt atttaatttt
      781 gaagataatc tgcccggcaa cccgcaaatg aattatacgg ttgcaacagc cgtcgaagcc
      841 tcggacggca aattattgct ttgcacaaaa ataaagaaaa tcgagaaaaa ttccgatggc
      901 taattgtaaa ttctcaatta ttacttctca attacttcac aaaagatacc gaacatcaat
      961 ttagaattta caatttagaa tttacaattt cccctttctt tatttttgtg caaagccgca
     1021 aattatggtt cgccacggat tcggggctgg cgcaaatcga cccggcaaat ctcacaaaga
     1081 atccgttgcc gccgccgccc gttataaaat ccatccagac ggatgagaag ctctaccgcc
     1141 tcgatcagga tttggttttt ccggcgggga cgctgccgca acaagccgct ttcaagagga
     1201 atagcttttc cccgaacaga taagaaaatt caatcgtcaa tgatttaagc gctaaccgct
     1261 tatgtccgtt attagaccaa ttcgataaag ccgcttcaaa gcatttttcg agcaaatcga
     1321 tatattaagc cggaaaacac gaggttactg ctgcgaaacc ggctcatcaa ggcggctcgc
     1381 cgattgatcg gattcaattc tcattagaac acgattcgat tcagacgacg gagaaatatt
     1441 taggcgttga gcaggattta actgccgccc catgcgatca tctcgggcta agaatttctg
     1501 cccgacaata tggatttcgc ttttgcaaat ggagtgcgaa aaaccctgaa tttgagaaga
     1561 gtttggagct gacttaaggc aaacgaacta tgaataattt cgcattccca ttaaagagtt
     1621 catattcgga tattccgaga taaatatggc agggttagat gcgaaatacg ctatacggaa
     1681 ggttaacggc gaggcttatg aaaacatacg gttaggaacg tataataaac agtaaaaaac
     1741 aaagctaagc tgtgaaattc gggaatttca cggcttcagc attttcggct ggtaagacgt
     1801 cgaggcgaat tctctcaaat cctgataagg attatcacta ttgacaagct cctggtttca
     1861 acgttgttaa aattatattg acattaccgc ccgaacctgt caggctcaag ctgccgtgga
     1921 atgactaaat accaaacccg cttaaataac ttcttcatag tccaataatc cattaaatcg
     1981 aatggagtgc aatcttggtt cccggaaaag cggaaagtaa atttccagac tcaaatttct
     2041 gagaaaaaat taactaaaaa aagctagaaa gctttggact ttttcgttca gccgcataaa
     2101 gcggcaatac ggaaaagtcc tctgtgaaga attatattat gagaatatta aaaaataccc
     2161 ttcaagcggg tttgctcgga atgtgcctgc tgctgggcgt cgccggctcg caagccgcgg
     2221 atttgacggt caatacaacg gctgacgccg ggatcggcag cctgcgccag gcaatcatcg
     2281 acgcaaccat taacgcggct gccaacacta ttaccttcaa cgtctcgacg accgacccgg
     2341 gctacaatgc cgcggagaat cgctttaccg tcaatttgtc gagctcgctg ccggacatcc
     2401 cgctcgcgcc tctgaccatc aacaatttcc agtctcaggg catcaccgta aagggcaaca
     2461 acagtttccg tatcttcacg ctggtcaaca gcgcggtcgt tacgatgaat aacctgacca
     2521 tcagcaatgg tttcagcagc gacggcttgg gcggcggcat tttcatgggc aacagcagca
     2581 cgctgaccct caacggaagc aacgtcagca acaataccgt ctccggcaac ggcggcggca
     2641 tctatatgcg cgacagcgcg acgctgaccc tcgccaattc gaccatcagc ggcaacaccg
     2701 cgggcaacgg cggcggtatc tatatcaatg acagcggcac gcttaacatc agcaccagca
     2761 ccgtttccgg taatacggct aataacggcg gcagcggcgg cggcgtcttt aacggcacca
     2821 gcggaacaat aaacgccgcc aacagcacgt ttgacgacaa ctcagccgcc aataacggcg
     2881 gagggattta taataccgcg acgattacgc tcaacagcaa taccgtcacc ggcaacaggg
     2941 ctgctaaggg cggcggcatc tacaacaatt tcaccgctac gctgaataac aacctcgtcg
     3001 cgctgaacgc cgcgaccgac ggtaacgacc tgctcggaag aggcagtctc ggcaacgctt
     3061 tcaacggcag ttacaacctg gttcgcaacg ccgacggcag cgagggatta ggcgcgagca
     3121 atcagctcgg gacgacctta aatcccatcg acccgcgcat cggacctttg aaaaataacg
     3181 gcggcgcgac ctttacgcga gcgctccata accgcagccc ggcgattgat aagggcaaca
     3241 gcccgagcct gattaccgac cagcgcggca aagcgcgccc gtacgacaat cccctggtcg
     3301 ccaacgcgcc gggcggtaac ggcgctgaca tcggcgcgtt tgaacgaacc ttcaccccga
     3361 cgccgttcga ttatgacggc gacgggcggg cggatgtttc ggttttccgt ccgtcagacg
     3421 gaacctggta tttgcagaaa tcgacggacg gtttttccgc tgtccagttc ggaatctcga
     3481 ctgataaatt agccgccgcg gactttgacg gcgacggtaa aaccgacatc gccgttttcc
     3541 gcccttcaaa cggaacctgg tatttgcagc aaagcacgaa cggttttgcc gccgtccaat
     3601 tcggcgcgag cggcgacatc gcgcagccgg cggatttcga cggcgacgga cgagccgagc
     3661 tggctgtttt tcgtccttca agcggcacct ggtttacctt gagtttagcc gacaatcaat
     3721 tcagcggcgt tcaattcggc aataacggcg ataagccggt cgcggctgat tacgacggcg
     3781 acacgaaagc ggattatgct gtttttcgcc cgtccgacgg cgtgtggtat tcgctcagaa
     3841 gccagtccgg atttacggct gttcaattcg gaatagcatc ggataagccg gtagctgccg
     3901 attatgacgg cgacggcaaa tcagaccagg cggtttatcg tgacggcgtt tggtatcagc
     3961 tcagaagcag tcagggcttt gcggctgtcc agttcggcac ggcgaccgac ttagccgcgc
     4021 cggcggattt cgacggcgac ggcaaggcgg atttagtcgt cttcag
//
LOCUS       DASWNE010000039         2492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_284054, whole genome shotgun sequence.
ACCESSION   DASWNE010000039 DASWNE010000000
VERSION     DASWNE010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284054"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VGB68_00835"
     CDS             complement(<1..209)
                     /locus_tag="VGB68_00835"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0047800.1"
                     /translation="MEKQTKVLVTGAGGFIGSHLVTYLQGKGFWVRGVDIKYPEFAET
                     TADEFEILDLRRWDNCLQATRGIEEV"
     gene            complement(322..>2492)
                     /locus_tag="VGB68_00840"
     CDS             complement(322..>2492)
                     /locus_tag="VGB68_00840"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEY0047801.1"
                     /translation="RPLAPNSDPAYFNTQLQLLTSDSILRRVVKELSLDNNKDFQVAK
                     AEDGDSTWQSMLRAVGLASKPEKKEDKGVAEVTLGSSLASNEDINEALRLAPYVDVIK
                     KKLRIEPVRESRATVKDTRHILISFQHTKPELAALVANGIGEVFTKVNQEKRTGKNKE
                     TNDFLTERVATLQAEIKRDELRLAELKNNAGILKKDEGQTIVIDRLAGLNKQLLDAEN
                     KRKNAEAEYNAIKSSPERIRALAEDKMVRYFTERENDIRTLTNEVTKKVADLEAEKER
                     LLIEYQENAPEIQEYNKKINSLRDTIQKRQEQNKKDLDEYRTRSARDIVTNLQTRYEQ
                     AKSEEDKIRAAFNEQRSEAQGENQAAINIRLLEQNVETNRGFLENLMKNQSQNDIQAR
                     GTDNNISVVDWALPPETAISPRRLTTVLAALFLSTLFGAGLALFFEYLDDTIKTTEEV
                     ENFLQLPALAAIPTIDSMPKQRRLLLVGANEEADRTGKTELLIHTDSRSALAEAYRQL
                     RTSILLSTAGHAPKSLLITSSLPSEGKTTTAVNTAISLAQTGASVLIIDSDMRRPRLH
                     SIFNISNSEGLSTILSSELSEEEVLDIVQYEETAKLHLLPSGPVPPNPAELIGSEQMS
                     NLLKVMQKRFTHVVVDSPPIASFTDGVLIASMVDGVILVVHSGKSSRQVVRRSKQLLN
                     DIGAKIFGVVLNNVNLRSQDNYYYYQSYYHRSNYRHDDEQ"
BASE COUNT          497 a          655 c          630 g          710 t
ORIGIN      
        1 acttcctcga tgccgcgcgt cgcctgcaga cagttgtccc agcgacgcaa atcgaggatt
       61 tcgaattcgt cggcggtcgt ctccgcaaat tccggatatt taatatcgac gccgcgcacc
      121 cagaaccctt ttccctgcag atatgtcacc agatgactgc cgataaaacc gcccgcgccc
      181 gtcaccaaaa cctttgtctg tttctccata tattcgtgaa atttttctct tggtctctac
      241 aaacagtttt taagaccgcg aaaaatatac tcgcgtttct ttttgttttt tactctttta
      301 atttttaatt ttgccttcga tttattgctc gtcatcgtga cgataattcg aacggtgata
      361 atagctctga taataataat aattatcttg cgagcgcaga ttgacgttgt tcagcacgac
      421 gccgaaaatt ttcgcgccga tgtcgttgag caattgcttt gagcgcctga cgacctgccg
      481 cgagcttttg ccggaatgca cgaccagaat cacgccgtca accatcgagg cgatcaaaac
      541 tccgtccgta aacgaggcaa tcggcggcga atcgacgacg acgtgcgtaa agcgtttctg
      601 cattactttc aacaaattag acatctgttc cgacccgata agctctgccg gattcggcgg
      661 aaccggtccc gaaggaagca gatgcagctt ggcggtttcc tcgtattgaa cgatgtccag
      721 aacttcttct tcactcagct cgctggacaa aatcgtgctc aagccttcgc tgttgctgat
      781 attgaaaatg ctgtgcaggc gcgggcggcg catatcggaa tcgattatca aaacgctcgc
      841 gccggtctgc gccaaactga tggcggtatt gacggcggtc gtcgtcttgc cttcggacgg
      901 caggctggac gtaatcaaaa gcgatttcgg cgcgtgcccg gcggtcgaaa gcaaaatcga
      961 agttcgcagc tggcgataag cttctgccag cgccgagcgc gaatcggtgt gaatcaaaag
     1021 ctcggttttt ccggttctgt ccgcttcctc gttagcgccg accagtaaaa gacggcgttg
     1081 tttcggcatc gaatcaatgg tcgggattgc cgccagagcc ggaagctgaa gaaagttttc
     1141 gacttcttcg gtcgttttga ttgtgtcgtc cagatattcg aagaacaaag ccagaccggc
     1201 gccgaaaagc gtcgagagaa acagagccgc cagaacggtc gtcaagcggc gcggcgaaat
     1261 cgccgtttca ggcggcagcg cccaatcgac gacgctgata ttattatcag tcccgcgcgc
     1321 ctgaatgtcg ttctggctct ggttcttcat taaattttcc agaaaaccgc gattcgtctc
     1381 tacgttttgt tccagcaggc gaatgttgat ggcggcttga ttctcgccct gcgcttcgct
     1441 gcgctgctcg ttaaaagcgg cgcggatttt atcttcctcg gatttagcct gttcataacg
     1501 cgtttgcagg ttagtcacga tgtcgcgcgc cgaacgcgtc cggtattcgt ccaaatcttt
     1561 tttattctgc tcctgccttt tttgaatagt atctctcagg gaatttattt ttttattata
     1621 ttcctgaatt tcaggcgcgt tttcctgata ttcaatcagc agtctttcct tttcggcttc
     1681 gagatcggcg acttttttgg tcacttcatt cgtcagcgtc cgaatgtcgt tttcgcgctc
     1741 ggtaaaatag cgcaccattt tgtcttcggc taaagcgcgg attctttcgg gcgagctttt
     1801 tatcgcgttg tattcggctt cggcgttttt acgcttgttt tcggcgtcga gcagttgctt
     1861 gttcaatccg gcgaggcggt cgatgacgat ggtctgacct tcgtcttttt tcaggattcc
     1921 ggcattgttt ttcagctccg ccagtctcaa ttcgtcgcgt ttgatttccg cctgcaaagt
     1981 ggcgacgcgc tcggtcagaa agtcgttcgt ttccttgttt ttgccggtgc gtttttcctg
     2041 atttactttg gtaaagactt cgccgatgcc gttggcgacc agcgcggcaa gctccggttt
     2101 cgtgtgttga aaactgatta gaatatgccg cgtatcctta accgtcgcgc gcgattccct
     2161 gaccggctcg atgcgtaatt tctttttaat aacgtcgacg tacggagcca ggcgcagcgc
     2221 ttcattaata tcttcgttcg acgccagact gctgccgagc gttacctcgg cgacgccttt
     2281 gtcttctttc ttttccggtt tgctcgccaa gccgacggcg cgcagcatcg actgccaggt
     2341 cgaatcgccg tcttcggctt tcgccacctg aaaatctttg ttgttgtcga gactcagttc
     2401 cttgacgacg cggcgcagga tgctgtcgct ggtcaaaagc tgaagctgcg tgttgaaata
     2461 agccgggtcg gaattcggcg cgagcggtcg gt
//
LOCUS       DASWNE010000040         6675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_637672, whole genome shotgun sequence.
ACCESSION   DASWNE010000040 DASWNE010000000
VERSION     DASWNE010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6675
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637672"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VGB68_00845"
     CDS             complement(<1..267)
                     /locus_tag="VGB68_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047802.1"
                     /translation="MIPDMISSKHNSTNAGREISDRNSSSAILCDSLIGVWQGASSFG
                     ETGVVPMQGISAFSVFEIPSVPDLSSPRFADFSRLDKTQRISQMG"
     gene            complement(264..1469)
                     /locus_tag="VGB68_00850"
     CDS             complement(264..1469)
                     /locus_tag="VGB68_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047803.1"
                     /translation="MQKFIQLSALSKQNNPAGEIKIMSNSVDNILGLSSNAQTSSNAK
                     PCASCNREIPIESAICPFCGKSQVMQTMLPAQPPIVAPIQASNKQDSNFPFIEFRASD
                     NIWSFEEREPPVPPEQQRPVLILDEETILLKETHKYIPPDELLSRVRNIIAQQNVPVE
                     AIIAKARWLNDYMEVRPRIVASLRNHAYSGVKMILGLDYMGNWANLQFQIGMQPDPIP
                     KPPATATGNPLPILLILGGLFLGFIGFVAAAAGSGLGYLLLLAGIAGLVGGVYMHMQI
                     QQQVDVARREREKYEREQYQQNLYKARMSLFRTYKIDDVLLFREAMRAVFERVVDDII
                     EQGGGEKVREIKGGNNELFEKQATAHAAPQNAQDTTQIAHQPLSDQVKSAVTTVTKHQ
                     IKQKTGFEV"
     gene            complement(2067..2720)
                     /locus_tag="VGB68_00855"
     CDS             complement(2067..2720)
                     /locus_tag="VGB68_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047804.1"
                     /translation="MDTSELGHIIFSQSSDAPKDLQLREEILNLPDDNSEALKKLKEK
                     LQSNYVKFFKHPFKDENFQQKWEKLTKLRNKIAHNNLFTLKDLQEGQQMFRDVLEIIR
                     VVDKNLPNVIVSEEDLEVFQRNFDEVLFDISENEFLKRLKDRESYYYGSGAYVGLTNF
                     INDHLYANGFDKKSSFEVYNKLLDKGLIEVYEVPGIYKERMVQAIKTTEDFENKSFI"
     gene            complement(2768..2842)
                     /locus_tag="VGB68_00860"
     tRNA            complement(2768..2842)
                     /locus_tag="VGB68_00860"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2808..2810),aa:Asn,seq:gtt)
     gene            complement(2996..3559)
                     /locus_tag="VGB68_00865"
     CDS             complement(2996..3559)
                     /locus_tag="VGB68_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047805.1"
                     /translation="MKDKPNFYLVSALIMIALAATACSSSNSQAVADTTVNNQSSASR
                     NMNESTAKNVEVRTDIENLEKQINLPVRPQEVVWTAEIFDNSKGAVPGPTDYRLTALL
                     KYDDKGAAELVRMLRAEIIDTSLGNADVKSWFPDEIKKEAKTVDGRTYLEGEKYSPTS
                     FFKSNYRNGNLIRLGKTNYFVLNVFSF"
     gene            complement(3594..4652)
                     /locus_tag="VGB68_00870"
     CDS             complement(3594..4652)
                     /locus_tag="VGB68_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047806.1"
                     /translation="MGLIDAIRQIKPVNNEANNQRVNPPARDAGTSEPAQTAFRTKAY
                     EQDFAARYTLAKLNFRRTPNTPAPTPAPQTPNLKTSGSGTETTYRIGSPTRPNIQHDN
                     GFLQNPKNPNDPKPMGTVAPSQVDIDYYNDQLNQVTWAQRIDNINIPFRDEDDTARRL
                     EDGIEAYRHFLEGKGADRNFSYEEFVSEDASGQTVLNNAIRDTQRGAEDLYNQMVARD
                     PSLAGKPITFNVTSGAITVGGSDAYPYPATENWQKAIGGHPIWNSATVTVNPPTQAGG
                     RPQFSMNYTLHAEDRYNFNPGQADIATGVPDATRGKLEQTGLAHQYTQYATLNRSVSW
                     TQGNISGTTRTTNPNEGR"
     gene            4865..6412
                     /gene="guaA"
                     /locus_tag="VGB68_00875"
     CDS             4865..6412
                     /gene="guaA"
                     /locus_tag="VGB68_00875"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEY0047807.1"
                     /translation="MQHELILILDFGSQYTQLIARRVREQGVYCEIVPFTFPLEKIVG
                     RKPKGVILSGGPNSVYEADAPRLAADFYNQIDVPLLGICYGMQLLAKDLNGQVEPSAK
                     REYGRAKLKVTNAKAKLFRDIHSEIEVWMSHGDHVVTVPSGFETVASSDGGAITAIED
                     TERRIFGLQFHPEVAHTPEGVEILRRFLFDVCECRGDWTSAQFIREEIEKIRAAVGDS
                     DNVVCGLSGGVDSTVAATLVHEAIGNRQACIFVNNGLLRADEFEDTLALYKEKLHLNV
                     RGVDASAEFYEVLKGVSEPEQKRKAIGRKFIEVFDAEAHRIGDVKYLVQGTLYPDVIE
                     SVSLRGASVTIKSHHNVGGLPEKMNLKLIEPLRELFKDEVRLIGKDLGIPPEILERHP
                     FPGPGLAVRILGDVTPEKVELLQRADRIFIEELRNFNLYNEVWQAFAVLLPIQSVGVM
                     GDFRTYERAVALRAVTSTDGMTADWARLPHDFLQAVSSRITSEIRGINRVVYDISSKP
                     PSTIEWE"
BASE COUNT         1662 a         1566 c         1546 g         1901 t
ORIGIN      
        1 tcccatttga gaaatgcgtt gtgttttatc aagtctggaa aaatcagcaa aacgtggaga
       61 actcaaatcc ggcacggatg ggatttcaaa aactgaaaac gctgatatcc cctgcatcgg
      121 cacgactccg gtttcgccaa agctcgatgc cccctgccat acaccaatta gcgaatcgca
      181 aagaatcgca gatgatgagt ttcggtcgga aatttctcgt cccgcgtttg ttgaattgtg
      241 ctttgaagaa atcatgtcag gaatcaaact tcaaaccctg ttttttgctt tatttgatgt
      301 ttggttaccg tcgttactgc cgacttcact tggtcactta atggttgatg ggcaatttgt
      361 gtcgtgtcct gcgcattttg cggggcagcg tgtgccgtcg cttgtttttc aaaaagctcg
      421 ttgttgccgc ctttaatttc tcttactttt tcgcccccgc cctgttcgat aatgtcatca
      481 acaacccgtt cgaaaaccgc ccgcatggct tcgcggaaaa gtaagacgtc gtcgattttg
      541 taagtgcgaa acagcgacat tcgagctttg taaaggtttt gttgatattg ttcacgctca
      601 tatttctcgc gttcacgacg cgccacgtca acttgctgct gaatttgcat gtgcatataa
      661 actccgccga caagtccggc aatcccggct aaaagcaaaa ggtagccaag cccgcttcct
      721 gccgccgctg caacgaaacc gataaatccc aaaaacagtc cgccgagaat cagcaaaata
      781 ggaagcggat tgcccgtagc agtagccggt ggtttaggga ttgggtcggg ctgcattcca
      841 atttgaaact gtaagttagc ccaattaccc atataatcta agcccaaaat cattttgact
      901 ccgctgtaag cgtgatttct tagcgaagct acaatgcgcg gtcgtacttc catataatcg
      961 ttaagccagc gcgccttcgc gataatggct tcgactggaa cattttgctg ggcaatgatg
     1021 tttcgcaccc tacttaacaa ctcatccggc ggaatgtact tatgagtttc tttgagaaga
     1081 atagtctcct catccaaaat caaaaccggg cgctgttgtt cgggcggtac aggcggttcg
     1141 cgttcttcaa aactccaaat attatcgctg gcacgaaatt caataaatgg gaaattcgaa
     1201 tcttgtttgt tacttgcttg aataggagcc actatgggcg gttgcgctgg taacattgtt
     1261 tgcatcactt gggatttacc acaaaagggg cagattgctg attctatcgg tatttcacga
     1321 ttgcaagaag cacatggttt tgcgtttgaa gaagtctgtg catttgatga taaccccaat
     1381 atattgtcga ctgaatttga catgattttg atttctcctg ccggattatt ttgtttcgat
     1441 aaagcgctca attggataaa tttttgcaat aaaaaaaaga ctcattaaat cagcgcagag
     1501 aatcaatgaa agagaattta atccccttta caaaaattct acttcgcttc ttagcagttc
     1561 ccctcaaatg ctaattagat ttaacttgtc gtcggttgaa gtgaatgtaa ttgattattg
     1621 aattggtgtc aatagttttt tctctttatt aactaaaatt catgaaatgc aaattagaac
     1681 gtctactttg ttgaaaacaa tcagtcggtc attcggttaa tcagaaccga atgaccggct
     1741 actataagat ttatccgttg gtttctttta cttcatcagc ttcgccgaaa gggaatacag
     1801 aactttaatg aaattcagca acgtctccga aaaaaggcgc tggtttggat attaccccgt
     1861 tagcgaaact cttttcttca aatctgctac gaacttcttg gtattgtttt gattaacatc
     1921 aaaactagtc tacttgatgt ttgattagat agaaaaattc gatggtcttg aaaagcaaaa
     1981 acacgcattg aatgtcaaga cataagatta tttttcgact ttaaatacat tgttaattga
     2041 atttatacag tataaattat ctttcactaa ataaaagatt tgttttcaaa atcttcggta
     2101 gtttttatag cttgcaccat tctttctttg tatattccag gaacttcata aacttctatt
     2161 aaacctttat ctagtaattt attatagacc tcaaaagagg atttcttatc gaagccatta
     2221 gcatacaaat ggtcgttaat aaaatttgtt aaaccaacat atgctcctga accataataa
     2281 taactttctc tatctttcaa ccttttcaaa aactcatttt ctgaaatgtc gaataaaact
     2341 tcatcgaaat ttctttgaaa tacttctaaa tcttcctcac ttacgattac attcggaaga
     2401 tttttatcta caacgcgaat tatttcgaga acatctctaa acatttgttg cccttcctgt
     2461 aagtctttta gagtaaataa attgttgtga gctattttat tccttaattt tgtgagtttt
     2521 tcccattttt gttgaaaatt ttcatctttg aatggatgtt tgaaaaattt tacataattt
     2581 gattgaagct tttcctttaa ttttttaagc gcttctgaat tatcatcagg caaatttaat
     2641 atttcctctc ttaactgtaa atcttttggc gcatctgatg attgtgaaaa aattatgtga
     2701 cctagttcgg atgtgtccaa aaagcgaaag gggcatcagc ttgatgcccc taaaccattt
     2761 aaaatattgg ctccgcaggt aagactcgaa cttacaaccc ttcggttaac agccgaatgc
     2821 tctgccattg agctactgcg gaataatcgc cttaaaaagg cgaattttta gattatggat
     2881 tttgctttat cttgtcaagc cgagcggaga aatgacaaga ccttaaattg aattgccgtc
     2941 ttcgacgacg caaaagcggt ttgaaaatcg tccttactca tttaccggca gaaaattaaa
     3001 atgaaaaaac attcaacaca aaatagtttg tttttccaag gcgaatcaaa tttccgtttc
     3061 gataattgga cttaaagaaa ctcgtcggcg aatatttttc gccttccaga tacgttctgc
     3121 cgtcaaccgt tttcgcttct tttttgattt cgtcgggaaa ccatgatttc acgtccgcgt
     3181 ttccgagcga tgtatcgatt atttcagccc gaagcattcg cactaattcc gccgcgcctt
     3241 tatcgtcata tttgagcaga gccgtcaggc ggtaatcggt cggaccggga accgcgcctt
     3301 tcgaattatc gaaaatctcc gccgtccata cgacttcctg cggacgcacc ggcaaattga
     3361 tttgcttttc cagattttca atatcggttc tcacctcgac gtttttggcg gttgattcat
     3421 tcatatttcg ggaagcgctc gattgattat taacggtcgt atcggcgacg gcttggctgt
     3481 ttgaagaact gcaagcggtc gccgctaagg caatcataat caaagccgac actaaataaa
     3541 aattcggttt gtctttcatt cagctcaatc ttaacaaacc acgttcggca aatctaacgt
     3601 ccctcgttgg gattcgtggt tctggtcgtt cccgaaatat tgccctgcgt ccacgaaacg
     3661 ctgcggttga gcgtcgcata ctgcgtgtat tgatgcgcca agccggtttg ttcgagcttg
     3721 ccgcgcgtcg cgtccggcac tccggtagca atgtccgcct gtcccggatt gaagttgtaa
     3781 cggtcttcgg cgtgaagcgt gtaattcatc gaaaactgcg gtctgccgcc cgcctgcgtc
     3841 ggcggattga ccgtcaccgt cgcgctgttc cagatgggat gcccgccgat ggctttttgc
     3901 cagttttccg tcgccggata cgggtaagcg tcgctgccgc cgaccgtaat ggctccgctc
     3961 gtcacgttaa aagtaatcgg cttgcccgcc aggctcgggt cgcgggcaac catctgatta
     4021 tacaaatctt ccgcgccgcg ctgcgtgtcg cgaatggcgt tgttcaaaac cgtctgaccc
     4081 gacgcgtctt cgctgacgaa ctcctcgtaa gagaagttgc ggtcggcgcc cttgccttcg
     4141 agaaaatgac gataagcctc gatgccgtcc tcgagccgac gcgccgtatc gtcctcgtcg
     4201 cggaaaggaa tattgatatt atcgattcgc tgcgcccagg taacctggtt cagctggtcg
     4261 ttgtaatagt cgatgtccac ctgcgagggc gcgacggttc ccatcggttt cgggtcgttc
     4321 gggtttttcg ggttttgcaa aaaaccgttg tcgtgttgaa tattagggcg cgtcgggctg
     4381 ccgatgcgat aagtcgtctc ggtgccgctg cctgaagttt tcaaattggg cgtctgcggc
     4441 gcgggcgtcg gcgcgggcgt attcggagtt cgccggaaat taagtttggc aagcgtgtag
     4501 cgagcggcaa aatcctgttc gtaagccttg gttctgaagg cagtctgagc aggctcggaa
     4561 gtgccggcat ctctggcagg cggatttacc ctctgattat tagcttcatt attaacgggt
     4621 ttaatttgtc tgattgcatc tataagtccc atctcaactc cttatgataa tcctgtagcg
     4681 tttacaaaaa aaatttccgc aaagtatttt ttatatttat cgacattata gttatcggca
     4741 gtttttgaga aagtttcctc tcggggcgca aaaaattctc aaacgctaaa caaaagattc
     4801 tgcatttgac ctcggacggc gttccgcatt agtttttaat ttacgattat tgcggaggtc
     4861 tcttttgcaa cacgaactta tcctgattct cgatttcggc tcgcaataca cgcagcttat
     4921 cgcgcggcgc gtgcgcgagc agggcgttta ctgcgaaatc gttccgttta cgtttccttt
     4981 ggaaaaaatc gtcgggcgca aaccgaaagg cgtgatttta tcgggcggac cgaattcggt
     5041 ttacgaagcg gacgcgccgc ggctggcggc ggatttttac aatcaaatcg atgtgccgct
     5101 gctcggcatc tgctacggga tgcagcttct ggcaaaagat ttgaacggtc aggtcgagcc
     5161 ttcggcaaaa cgcgaatacg ggcgcgcaaa gctgaaggtt accaacgcaa aggcgaagct
     5221 tttccgcgat attcacagtg aaatcgaggt ctggatgagc cacggcgacc acgtcgtcac
     5281 ggttccgagc ggttttgaaa cggtcgcttc gagcgacggc ggcgcgatta cggcgatcga
     5341 agacacggag cgaagaattt tcggcttgca gtttcacccg gaagtcgcgc acacgccgga
     5401 gggcgtggaa attttgcggc gctttttgtt tgacgtctgc gaatgccgcg gcgactggac
     5461 ttcggcgcag tttatcaggg aagaaatcga gaagattcgc gcggcggtcg gcgacagcga
     5521 taatgtcgtc tgcgggcttt cgggcggcgt cgattcgacc gtcgcggcaa ccctggtgca
     5581 cgaggcaatc ggaaatcgtc aagcctgtat cttcgtcaat aacggcttgc tgcgcgccga
     5641 cgaattcgaa gatacgctgg cgctttataa ggaaaaactg cacctcaacg tgcgcggcgt
     5701 cgacgcttcc gcagaatttt acgaagtcct gaaaggcgtc tccgagcccg aacaaaaacg
     5761 caaggcgatc gggcgcaaat ttatcgaagt tttcgacgcc gaagcgcatc gtatcggcga
     5821 cgtcaaatat ctggtgcagg gcacgcttta cccggacgtc atcgaatccg tatctttgcg
     5881 cggcgcgagc gtgacgataa aatcgcatca caacgtcggc ggcttgccag aaaaaatgaa
     5941 tctgaaactg atcgagccgc tgcgcgaatt gttcaaggac gaagtgcgcc tcatcggtaa
     6001 agacttaggc atcccgccgg aaatcctcga acgccacccg tttcccggtc ccggcttggc
     6061 ggtgcgtatt ctgggcgatg tcacgccgga aaaagtcgag cttttgcaga gagccgaccg
     6121 catttttatc gaagagctgc gcaatttcaa tctctacaac gaagtctggc aagccttcgc
     6181 cgtgcttttg ccgattcaat cggtcggcgt aatgggcgat tttcgcacct acgagcgcgc
     6241 cgtcgcgctg cgcgccgtca cctcgaccga cggaatgacc gccgactggg cgcgcctgcc
     6301 gcacgatttt ctgcaagcgg tctcctcgcg catcacgtcc gaaattcgcg gcatcaaccg
     6361 cgtggtttac gacatttcct caaaaccgcc gagcacgatt gagtgggaat aatttaaaaa
     6421 tttaagattt accggtttga tagatatttg cgtccgagtt ttaacgaatg ctcttcaaac
     6481 cggttatatt taatttactc tagaaaaatt cgtcgagcag gcggtagaaa cgaatctttt
     6541 tccagtcggg ctcgatgccg tagattttaa agacgttttg ctcgtattcc ctgccgaaat
     6601 tgtatataac gctgcgcgtc agcaacgcga tgtcctggta gcggtcggcg atgcctgcgt
     6661 ttccgcaatc gacaa
//
LOCUS       DASWNE010000041         3027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_248936, whole genome shotgun sequence.
ACCESSION   DASWNE010000041 DASWNE010000000
VERSION     DASWNE010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248936"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1362)
                     /gene="lepA"
                     /locus_tag="VGB68_00880"
     CDS             complement(<1..1362)
                     /gene="lepA"
                     /locus_tag="VGB68_00880"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEY0047808.1"
                     /translation="MNSEFIRNFSIIAHIDHGKSTLADRILQLTGAVSDRDMEEQLLD
                     DMDLERERGITIKAHAVRLDYKARDGQNYVLNLIDTPGHVDFSYEVSRSLSACEGALL
                     VVDASQGVEAQTLANTYLALENDLELIPVLNKIDLPSAEPDRIKEQIENIIGLDTSHA
                     VLASAKMGIGIEDILEQIIKDVPAPKGDPDAPLKALIFDSWYDSYRGVIVLFRIIEGT
                     IKKGTKIRFFNTGREYLVEMIGVNRPRPTPIGSLSVGEVGFLTASIKTVADVQIGDTI
                     TEAARPVDTPFPGFQEVKPMVFAGLYPTDSAQYEDLRDAMDKLRLNDASFFYEPESST
                     ALGFGFRCGFLGLLHMEIIQERLEREFNLDLITTAPGVRYRVTTTNGEVTEVDSPSKM
                     PETGRIARIEEPYIEATILTGDAFLGGILALLEEKRGVQKKFEYVTENRVMLIYELPL
                     NEIV"
     gene            1642..2379
                     /locus_tag="VGB68_00885"
     CDS             1642..2379
                     /locus_tag="VGB68_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047809.1"
                     /translation="MSEFLPAFSAASTFAILGFGFLLGLKHATEADHLAAVSTIVTER
                     RSLVGSAIIGGLWGLGHTISLFAAGVFVLLLNFQISERTERLLEFGVGVMLVLLGLNV
                     LRKLIQGGHLHFHAHEHGRRVHAHPHIHEPAADEPHTHHGFSFSPRALIIGMIHGLAG
                     SAALMLLIIPTIESQAVGLLYIIVFGVGSIGGMMLMSLLVGLPFTLTASRFNRFNHIL
                     QSVAGLVSVAIGLFIIYEKGITEGLFS"
     gene            2715..>3027
                     /locus_tag="VGB68_00890"
     CDS             2715..>3027
                     /locus_tag="VGB68_00890"
                     /inference="COORDINATES: protein motif:HMM:NF023855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme N-terminal
                     domain-containing protein"
                     /protein_id="HEY0047810.1"
                     /translation="MISFGADICADFQNASSREWLETNGIGGFASGTLAGANTRRYHG
                     LLTAATKPPLGRLTLLSKFEETLFIDGEAFELSSNQYPNKIYPEGFRYLKSFRLAPFP
                     VW"
BASE COUNT          673 a          777 c          798 g          779 t
ORIGIN      
        1 cacgatttcg ttgagcggca attcgtaaat aagcattacg cgattttccg tgacgtattc
       61 gaactttttc tggacgccgc gcttttcctc cagaagcgcc agaattccgc cgagaaaggc
      121 atcgccggtt aaaatcgtcg cttcgatata cggttcttcg atacgagcga ttctgcccgt
      181 ttccggcatt ttcgagggcg aatcgacttc ggtcacctcg ccgttggtcg tcgtcacgcg
      241 atagcgcacg ccgggcgcgg tcgtaatcaa atcgagattg aattcgcgtt cgagccgttc
      301 ctggatgatt tccatatgca gaagtccgag aaagccgcag cggaagccga aaccgagcgc
      361 ggtcgaggat tccggctcgt agaaaaacga ggcgtcgttc aggcgaagct tgtccatcgc
      421 gtcgcgcaaa tcttcgtatt gcgccgaatc ggtcggataa agcccggcga aaaccatcgg
      481 cttgacttcc tgaaagccgg gaaaaggcgt gtcgacgggt cgcgccgctt ccgtgattgt
      541 gtcgccgatt tgcacgtcgg cgacggtttt tatcgaagcg gtcagaaatc cgacttcgcc
      601 gaccgacagc gagccaatcg gcgtcgggcg cgggcgattg acgccgatca tttcgaccag
      661 atattcgcgt ccggtgttga agaaacgaat tttcgtgcct ttttttatcg tgccttcgat
      721 gatgcgaaac aaaacgatga cgccgcgata cgaatcatac cagctgtcga aaatcagggc
      781 tttcaaaggc gcgtcggggt cgcctttcgg cgcgggaacg tccttgatga tttgttcgag
      841 aatgtcttcg atgccgatgc ccattttcgc cgaagccaga acggcgtggc tcgtatccag
      901 cccgatgatg ttttcgattt gttccttgat gcggtcgggc tcggcgctcg gcaggtcgat
      961 tttatttaaa accggaatca gttcgaggtc gttttcaagc gccagatatg tgttcgccag
     1021 cgtttgcgct tcgacgccct gcgaagcgtc aactactaat aacgcgcctt cgcaagccga
     1081 gagcgagcgc gaaacttcgt agctgaaatc gacgtgaccg ggcgtgtcaa tcaagtttaa
     1141 aacgtaattc tgcccgtcgc gcgccttgta atcgagccgg acggcgtgag ctttaatcgt
     1201 gatgccgcgc tcgcgctcca aatccatatc gtccaataac tgttcttcca tatcacggtc
     1261 gctgacggcg cccgtcagct ggagaatgcg gtcggcgagc gtcgatttac cgtggtcgat
     1321 gtgcgcgatg attgaaaaat ttctgataaa ttcggaattc atagcttaat aagaaaacat
     1381 tttggtgaaa aaaccaaaag ataacaatag caaaacccgc gccgattcgc acccgcagcg
     1441 gattgcggaa atatttcctt taatttatta aaatccctgc ctccgttttc aaagaggcgg
     1501 cgaatccgtc cgcgtcggag aaaaattcgt tctgttttta aaagcggaaa aattgacggg
     1561 tcaaaaccgt tccgatttgc agcgcgccgg cgagcgcctg cgtcggcgcg ataatctgtt
     1621 ataattcagt ggcataaaat catgagcgaa tttttaccgg cgttttccgc cgcttctact
     1681 tttgcaatct taggtttcgg ttttctgctc ggcttaaaac acgcgaccga agccgaccat
     1741 ctggcggcgg tctcaaccat tgtcaccgag cgccggagtc tggtcggctc ggcgattatc
     1801 ggcggtttgt ggggcttggg gcataccatt tcgcttttcg cggcgggcgt tttcgttctt
     1861 ttgctgaatt ttcaaatcag cgagcgcacg gaaagacttc ttgaattcgg cgtcggcgtg
     1921 atgctcgttt tgctcggctt gaacgtttta agaaaattga ttcagggcgg gcatcttcat
     1981 tttcacgcgc acgaacacgg gcggcgcgtt cacgcgcatc cgcatattca cgagcccgcg
     2041 gcggacgaac cgcacacgca tcacggattt tccttcagcc cgcgcgcttt gattatcggg
     2101 atgattcacg gtctggcggg cagcgccgcg ctgatgctgc tcataattcc gaccatcgaa
     2161 tcgcaggcgg tcggcttgct ctatatcatc gttttcggcg tcggctcaat cggcggaatg
     2221 atgctgatga gccttctcgt cggtttgccc tttacgctga cggcttcgcg ctttaaccgc
     2281 ttcaatcaca ttctgcaaag cgtcgccggt ttagtcagcg tcgctatcgg tctttttatc
     2341 atttacgaaa agggaattac cgaaggtctg ttcagctaga agcgattcag ccgcgaacaa
     2401 acacgaaaga acactgacaa ggaatgaaga aaatttaccg cacgcaacgc agggaaagcg
     2461 cagagaaaag caaatggaaa tttacaggga taagcgagct tcgcggcttg ataaatgggc
     2521 ataagaagaa tggtttttat ttatcttcgg ttcatcccgt caagcctgta aattcctttt
     2581 tcgcctcttt tcgcctcttt tcgcggctaa cttcgttttg ttcgcggctg aattcttgca
     2641 gcttttttaa atgtaagcta gtcttttaga gcctttggaa taatcatcag acaaaaagga
     2701 ataacttagc cggaatgatt agcttcggtg cggacatttg cgcggatttt caaaacgctt
     2761 cttcgagaga atggctcgaa accaacggca tcggcggttt tgcttccggc acgctggcgg
     2821 gcgcgaatac gcgtcgttat cacgggcttt tgacggcggc tacaaagccg ccgctcggtc
     2881 gcctgacgct gctttccaag tttgaagaaa ctctttttat cgacggcgag gcgtttgagc
     2941 tttcctcgaa tcaatatccg aacaaaattt atcccgaagg ctttcgatat ttaaaatcgt
     3001 ttcgcctcgc gccgtttccc gtttgga
//
LOCUS       DASWNE010000042         7428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_284233, whole genome shotgun sequence.
ACCESSION   DASWNE010000042 DASWNE010000000
VERSION     DASWNE010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7428
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284233"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VGB68_00895"
     CDS             <1..234
                     /locus_tag="VGB68_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047811.1"
                     /translation="MAKLQRIVSVTDFNLKQLEKQSPDKTLHAQFLLTAYYASPENLT
                     ATTNPQQPNGTAPTTPGQPVAPNAQTPPAPVAK"
     gene            293..1144
                     /locus_tag="VGB68_00900"
     CDS             293..1144
                     /locus_tag="VGB68_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047812.1"
                     /translation="MLRFLSSSVAAAGFLLIAGSVMPAGAQQGRDPFTKLGWAKPRDP
                     NAKTTTVTTKDGRTVVKPVPVAPAPVVPPAIQERINYYKRLRETAVANNQPIPKVTSV
                     LTLDEMAVTGIFRTPRGYAAMVEAKPIKLSYTIYPGEKFFDGQLVAIEENKLVFRRVT
                     KWTNGKFIASEESKSLRQYTVEQEVNGTAPVETTTTAKTETTPAQTPAQTPAQTPANT
                     AENKPVVMPSPAPIVSPLDEMARQPAENPKEAAKKSTDKTKKGKQKSSVKTTEKKAVK
                     VADNKQQ"
     gene            1283..2950
                     /gene="pilQ"
                     /locus_tag="VGB68_00905"
     CDS             1283..2950
                     /gene="pilQ"
                     /locus_tag="VGB68_00905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEY0047813.1"
                     /translation="MTFLQKLSSFGIRAALALMVIGVFALAVSAQKVGTPRQGTDYGK
                     PGFIGEPINLNVVNADIRDILNYITEQYGVNFVIDKSVKNVPVTVNVSDVPWNIALDS
                     ILQSQDLGVQVNGPILRVAERQTLANEQAVANKIRESQLDSSPLYTEFIRLNYACAGA
                     CASQTSFQGGTSSGGGGGGGGTDQGILGVVKKRLSRRGAVEVDNRSNTLIITDVRENI
                     DAIRQLILLLDNPEPQVEIEARIVIASRNFSRDLGAQLSAVLLGRGGSGGGFSTLPLE
                     APEEGGTGQNGGLLQVPGNVNPNGSLQSQLANSVLGLTTGIFGTAQINLLITAGENKG
                     QAKTIATPRVTTLNNRKAEIESGTQIPIVTTQPGTAGTAVALTTEYVSVPLRLEVTPQ
                     ITDVGTVVLDIVAENNSVGRLIGVGGTPAINTQRMKTYVTVPDGGTTIVGGALLDAEG
                     EDQFRTPGLSQVPILGNLFKRKAVSRTTNEILFFITPRIYRPDFNGNQLPSKVSDGTR
                     TTTILQPVPLGNPQSNSSPTTNTMQQQQPVLPVVPVAEPSPNNPSRP"
     gene            2970..3338
                     /locus_tag="VGB68_00910"
     CDS             2970..3338
                     /locus_tag="VGB68_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEY0047814.1"
                     /translation="MFREVLHDVMRRTEGCLGALIMGTDGIVVEKVWQFGALAEMNLD
                     VAAAEFASLIKNAHRAGSELELGKLHEMTFTGEGGVFLLRFVSEDYFVAAILSPEGNF
                     GRARYELRRAELLLEKELAT"
     gene            3751..4362
                     /locus_tag="VGB68_00915"
     CDS             3751..4362
                     /locus_tag="VGB68_00915"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY0047815.1"
                     /translation="MKNIRINKKITLNRQSGVSLPELVIVFFVIAILVVLALPQLISS
                     RRALRFSGMQRQIVSSLREARQEAMSQRTAITFRYDDADKKIVVYGGSFGALGNTKNR
                     LVTMAGEGLLPGEVIYGRPAGAPVSALADSANMTSLSANAVEIKFQADGSVVDASDIP
                     INKALFFYNSKNPSETAFAVSVLGAGGRAKVWRYSRGVNAYVE"
     gene            4352..4945
                     /locus_tag="VGB68_00920"
     CDS             4352..4945
                     /locus_tag="VGB68_00920"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY0047816.1"
                     /translation="MSNNPEQKKNSNSTNRDEKGFSLIEAVVAILIITIGLIGTAAAI
                     TYALEFSAISRNVSNAKLVIVSTIEEIESLRNTRRLDFKQIANVGGVDNNGTPNTFNG
                     FSTGYKEVSLNPGPDGVNGTDDDLRSAGADGTYGTGDDADDPTLVRSGYVRQITITNL
                     NTTLKKVEIKVRYYGSGGKIGEIRGVSYINDEARITR"
     gene            4968..>7428
                     /locus_tag="VGB68_00925"
     CDS             4968..>7428
                     /locus_tag="VGB68_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047817.1"
                     /translation="MKSGIKIKDKKRNERGVALAIAVIIVAIMAVVALTALTFSSTEI
                     RIAGGDLQRTQAFYASSSALEKMNHDFSDLFRSKMRPTDADLSLIENSPPPELVTEGY
                     FFDQSLDEDHARLNALRAMQNLPATTYPRVNIPEGPYAGLYATIVPYKMSSKATSIYN
                     GTQVKLEREFNNYLVPLFQFGIFSNEDIEIHPGPLMTFNGRIHTNGNLYALRNTKFLN
                     RVTMAGEFIRDATRGGEPNTQSGNENVWFEVNSINVQSTLGNGSMTSGSGTVGGPNLP
                     GSTPGTRGYFPGSPNGIGNPGWESLSVRSPINGTPNRFGGQVLTNTTGATQLKMPLEL
                     DGSSPAELIKRTLPSDSEILAASRYHSKSQVRILIDDESAGTGAANVAGIPAGKGVLL
                     SAFSPLPLGNGSVLKRVDDSGSYVSSDTLITQKTGSGNQTAMTVRGVKSGIDTAPGYY
                     IPAGAGIRGRILIEVTKPDGTTVDVTQTILSMGMTEGEPNGIVYLQRPLWAAFVQGSR
                     DREGNRFDLTYLTNNTQMSADGEIKDNGSLFNATRGFITGNLNSLDDDGGSISREATA
                     TDYNQIVPINVYNVREGWFRSQLSEFDIYERGMTGIVEINMRNLARWLDGLYDANLLS
                     GTNAVSSNIQGTEGYVVYVSDRRGDRVKTEYKRDGSQYQSTNGFVDNEDIYGPNNTLD
                     AGEDVIDYGWDSTRGGANKKGTLQKDTQELPDLGTTWGAPVLPADAAARRLRAETVSK
                     WTNPNNYFRRGVRLFNAEKLSFTAASGKLSPTKGITIASENMVYLWGNYNATGITGIP
                     SGGSTLNTGGFTGAQVPSSIVC"
BASE COUNT         1871 a         2077 c         2011 g         1469 t
ORIGIN      
        1 atggcgaaac tgcaaagaat cgtctcggtc accgacttca atctgaaaca gctggaaaaa
       61 cagtcgcccg acaaaacgct ccacgcgcag tttttgctga cggcttatta tgcttcgccc
      121 gaaaatttaa cggcgacgac caacccgcag cagccgaacg gaaccgctcc gaccacgccc
      181 ggacagcccg tcgcgcccaa cgcgcagacg ccgcctgctc ccgttgccaa ataaatctaa
      241 aagctaaaga gttttgttga agagagaaaa agccatgatg aaaaaatcga aaatgttacg
      301 tttcttaagt tcaagtgtcg ccgccgcggg ctttctgctc atcgcgggaa gcgttatgcc
      361 cgccggcgcg cagcagggtc gtgacccgtt taccaaatta ggctgggcga aaccgcgcga
      421 cccgaacgcc aaaacgacga ccgtgacgac caaagacggc agaacagtcg tcaaacccgt
      481 tccggtagcg cccgcgccgg tcgtgccgcc cgccatccag gaacgcatca attattataa
      541 acgcctgcgc gagacggcgg tcgccaacaa tcagccgatt ccgaaagtga ccagcgtgct
      601 gacgctcgac gaaatggcgg tcaccggaat ttttcgcact ccgcgcggat acgctgcgat
      661 ggtcgaagcc aagccgataa agctttccta cacaatctat ccgggcgaaa agttttttga
      721 cggtcagctc gtggcgattg aagaaaacaa gctcgttttc cgccgcgtga cgaaatggac
      781 caacggtaaa tttatcgctt ccgaagaaag caaatctctg cggcaataca ccgtcgagca
      841 ggaagtcaac ggcacggctc cggtcgagac caccaccacc gctaaaacgg aaaccacacc
      901 ggcgcagacg cccgcgcaga caccggcgca aacgcccgcc aataccgccg aaaacaaacc
      961 cgtcgtgatg ccctcgcccg ctccgatcgt ctcgccgctc gatgaaatgg ctcgtcagcc
     1021 cgcggaaaac ccgaaagagg cggctaagaa atcgacggac aaaaccaaaa aaggtaaaca
     1081 aaaatcttcc gtaaaaacaa cggaaaagaa agccgtcaaa gttgccgata acaaacagca
     1141 gtaatctaca aacagcagta atctatcaga catttccaaa cgcgcggtcg gaattctccg
     1201 gtcgcgcccg ataaaatcca attaaaaacc tatgacatat caatgtctgc caatgacagg
     1261 caagatttgt gagggaggaa acatgacttt tcttcaaaaa ctaagcagtt tcggcattcg
     1321 tgccgcgctc gctttaatgg taatcggtgt attcgctctg gcggtttccg cacaaaaagt
     1381 cggcacgccg agacagggca cggattacgg gaaacccggc ttcatcggcg aaccgattaa
     1441 tctgaatgtc gtcaacgccg acattcgcga tattcttaac tacatcaccg aacaatacgg
     1501 cgtaaatttc gtcatcgaca aatcggtgaa aaacgttccc gtgacggtca acgtcagcga
     1561 cgtgccgtgg aacatcgcgc tcgattcgat tctgcagtcg caggatttgg gcgtgcaggt
     1621 caacggtccg attctgcgcg ttgccgagcg ccagacgctt gccaacgagc aggcggtcgc
     1681 gaataaaatc agggaaagcc agctcgacag ctcgccgctt tacacggaat ttattcgtct
     1741 taattacgcc tgcgccggag cttgcgcttc gcagacgagc tttcagggcg gaacgtccag
     1801 cggcggcggc ggcggcggcg gcgggacaga ccagggaatt ctgggcgtcg tcaaaaaacg
     1861 tctttcgcgt cgcggcgcgg tcgaggtcga taatcgcagc aatacgctga ttatcaccga
     1921 cgtgcgcgaa aatatcgacg ccattcgcca gttgattctg ctgctcgaca atccggagcc
     1981 gcaggttgaa atcgaagcgc gcatcgttat cgcctcgcgc aacttcagcc gtgatttggg
     2041 cgcgcagctt tcggctgtct tattaggtcg cggcggcagc ggcggcggat tttcgaccct
     2101 gcctttagaa gcgccggagg aaggcggaac ggggcaaaac ggcggattat tacaggttcc
     2161 cggcaatgtc aatccgaacg gcagcctgca atcgcagctc gccaactcgg tgctcggctt
     2221 gaccaccggc attttcggaa cggcgcaaat caatttgtta attaccgccg gcgaaaataa
     2281 aggacaagcc aaaaccatcg ctacgccgcg cgtgacgacg ctgaacaatc gcaaagccga
     2341 aatcgaaagc ggcacgcaga ttcccatcgt caccacgcag cccggaacgg ctggaacggc
     2401 tgtcgcgctg acgaccgaat acgtttccgt gcccttgcgt ctggaagtta cgccgcagat
     2461 aacggatgtc ggaacggtcg ttctggacat cgtcgccgaa aacaactcgg tcggacgtct
     2521 tatcggcgtc ggcggaacgc ccgccatcaa cacgcagaga atgaaaacat acgttactgt
     2581 tcccgatggc ggcacgacga ttgtcggcgg cgcgctgctc gacgccgaag gcgaagacca
     2641 gttccgcacg ccgggcttgt cgcaggttcc gattctcgga aatcttttca agcgcaaagc
     2701 cgtctcgcgc acgacgaatg aaattttatt cttcattacg ccgcgcattt atcgccccga
     2761 ctttaacggc aatcaactgc cctcgaaagt cagcgacgga acccgcacga ctaccatcct
     2821 gcagccggtt ccgctcggca atccgcagtc gaactcaagc ccgacgacca acacaatgca
     2881 gcaacagcag ccggttcttc cggtcgtgcc ggtcgccgag ccgagtccga ataatccttc
     2941 gagaccgtaa tttacaaacg gagtgtttag tgttcaggga agttttgcac gacgtaatga
     3001 ggcgcaccga gggatgtctc ggtgcgctga taatgggaac ggacggaatc gtcgtcgaga
     3061 aagtctggca attcggggct ttggcggaaa tgaatctgga tgttgccgcc gccgagttcg
     3121 cctcgctgat taaaaacgcg catcgcgcgg gcagcgagct ggagctgggc aagcttcacg
     3181 aaatgacttt taccggcgaa ggcggtgttt ttctgctgcg ctttgtcagt gaagattatt
     3241 ttgtcgccgc gattttatcg ccggaaggaa acttcgggcg cgcccgttac gaactgcgtc
     3301 gcgcagagct tctcctggaa aaagaactcg ccacttaaat tgtttctttc ctcttcccgt
     3361 cagaaccggc tcgcttctga cgggaatttt tttctctcct gaaaagcccg accggcgcaa
     3421 taatatatta aaggcggaaa ggcggacggc ttgttaaact ataaacgcga agcgataaac
     3481 gatgaattcg atgttccgaa gctgcgattc cgctttccgc gttcggcatt catcattccg
     3541 aagccgcgct gatcggacgc ccgcgttgtc cttaaaatca acctcgctct gattaatccg
     3601 aataattctc aaaatccata agcattgttt caaaagaatt atcagaaata tcgcaaaata
     3661 tttttcgtga taaacagcgt ttttactgga acaacaaact tattcaatct tatggtgagt
     3721 agagattatt ttcaagggat ttgaaaagcg atgaaaaaca tacggattaa taaaaaaata
     3781 actcttaatc gccagagcgg cgtcagcctg ccggagcttg taatcgtttt tttcgtcatt
     3841 gcgattttgg tcgtgctggc tttgccgcaa ttaatatcgt cgcggcgcgc gcttcgtttt
     3901 tcgggaatgc agcggcagat tgtcagttcc ctgcgcgagg cgcggcagga agcgatgtcg
     3961 cagcgcacgg cgattacatt ccgctacgac gacgcggata aaaagattgt cgtttacggc
     4021 ggcagcttcg gcgctctggg aaatacaaaa aatcgtctgg tcacgatggc gggcgaaggc
     4081 ttgctgccgg gcgaagtgat ttacggtcga ccggcgggcg cgccggtttc ggcgcttgcc
     4141 gacagcgcga atatgacttc gctttccgcg aacgcggtgg aaatcaagtt tcaggcggac
     4201 ggctcggtcg tcgacgccag cgatattccg ataaacaaag ctttattttt ctataacagc
     4261 aaaaatcctt ccgagacggc ttttgcggtt tccgtgctcg gcgcgggcgg gcgcgcgaaa
     4321 gtctggcgct acagccgcgg cgtgaatgct tatgtcgaat aatcctgaac agaaaaaaaa
     4381 ctcgaattcc acgaaccgcg acgaaaaagg cttttcgctg attgaagcgg tcgtggcgat
     4441 tctgattatc accatcggtt taatcggaac cgccgccgcc atcacctacg cgctcgaatt
     4501 cagcgccatc agccgcaacg tcagcaacgc caagctcgtc atcgtctcga ccatcgaaga
     4561 aatcgaatcc ttgcgcaaca cgcggcggct cgatttcaaa caaatcgcca atgtcggcgg
     4621 cgtcgataac aacggaacgc cgaacacctt caacggtttc agcaccggct acaaggaagt
     4681 ttccttaaat cccggaccgg acggcgtcaa cggaacggac gacgatttgc gcagcgcggg
     4741 cgcggacgga acttacggca cgggcgacga cgcggacgac ccgacgctgg tcagaagcgg
     4801 ctacgttcgc caaatcacta ttacgaatct gaacacgacc ctgaaaaaag tcgaaatcaa
     4861 agttcgatat tacggttcgg gcggaaaaat cggcgagatt cgcggcgtct cctatataaa
     4921 cgacgaggcg cgcatcacca gataaatttc caaaggcgga aaaggcaatg aagtcaggaa
     4981 ttaaaataaa agacaaaaaa agaaacgagc gcggcgtcgc gctggcgatt gccgtgatta
     5041 tcgtagcgat tatggcggtc gtggcgctga cggcgctgac cttttcttcg accgaaatca
     5101 gaattgcggg cggcgatttg cagcgcacgc aagccttcta cgcttcgtcc tcggcgctcg
     5161 aaaaaatgaa tcacgatttc agcgatttgt ttcgcagcaa gatgcgcccg accgacgcgg
     5221 atttgagcct aatcgaaaac tcgccgccgc cggagctggt cacggaaggc tatttcttcg
     5281 accaatcgct cgacgaagac cacgcgcggc tgaacgcgct gcgcgcgatg cagaatctgc
     5341 ccgcgaccac ttacccgcgc gtcaatattc cggaaggacc ttacgccgga ctttacgcga
     5401 ccatcgtccc gtacaaaatg tcttcaaagg cgaccagcat ttacaacgga acgcaggtca
     5461 agctcgaacg cgaatttaat aattatctgg ttccgctgtt tcaattcggt attttcagca
     5521 acgaggacat cgagattcat cccggtccgc tgatgacttt taacggcaga attcacacca
     5581 acggaaacct ctacgccctg cgcaacacaa aatttctgaa ccgcgtgacg atggctggcg
     5641 aatttatccg cgacgcgacg cgcggcggcg agccgaacac gcagagcgga aacgaaaacg
     5701 tctggtttga agtcaacagc atcaacgtgc agagcacttt gggcaacggc agtatgacgt
     5761 ccggttcggg aaccgtaggc ggaccgaatc tgccgggcag cacgcccgga acacgcggct
     5821 actttcccgg cagcccgaac ggaatcggca atcccggctg ggaatcgctt tccgtccgct
     5881 cgccgatcaa cggcacgccg aaccgcttcg gcggacaggt tttaaccaac acgaccggcg
     5941 cgacgcagtt gaaaatgccg ctcgagctcg acggcagctc gcccgccgaa ttgataaaac
     6001 gcacgcttcc ttctgacagc gagattctag cggcttcgcg ttatcattcg aaatcgcagg
     6061 tgcggatttt gatcgacgac gagtcggcgg gcacgggcgc ggcaaacgtc gccggcatac
     6121 ccgccggcaa aggcgttttg ctttcggctt tttcgccttt gccgctcgga aacggcagcg
     6181 ttttaaaacg ggtcgacgat tcgggcagct acgtttcgag cgacacgctt atcacccaga
     6241 aaacaggcag cggcaaccag acggcgatga ccgtgcgcgg agttaaatcc ggcattgata
     6301 ctgcgcccgg ttattatatt ccggcgggcg cgggcattcg cggcagaatt ttgatagaag
     6361 tcaccaagcc ggacgggacg accgtcgacg tgacgcagac gattctcagt atgggaatga
     6421 ccgagggcga gccgaacggc atcgtttatt tgcagcgccc gctgtgggcg gcgtttgtgc
     6481 agggaagccg cgaccgcgag ggaaatcgct tcgatttgac ttacctgaca aacaacacgc
     6541 agatgagcgc ggacggcgaa atcaaagaca acggctcgct ttttaacgcg acgcgcggtt
     6601 ttattaccgg aaatttaaac agtctcgacg acgacggcgg aagcatctcg cgcgaagcga
     6661 cggcaaccga ctacaaccag attgtcccga taaatgttta caacgtccgc gaaggctggt
     6721 tcagaagcca gttaagcgag tttgacatct acgaacgcgg aatgaccggc atcgtcgaaa
     6781 tcaatatgag aaatctggct cgctggctcg acgggcttta cgacgccaat cttttgtcgg
     6841 gaacaaacgc ggtcagcagc aatattcaag gcacggaagg ctacgtcgta tacgtttccg
     6901 accgccgcgg cgaccgcgtc aaaaccgaat ataaaagaga cggctcgcag tatcagagca
     6961 ccaacggttt tgtcgataac gaagacatct acggaccgaa caatacgctc gacgccggcg
     7021 aagacgtcat cgattacggc tgggattcga cgcgcggcgg agccaataaa aaaggaactt
     7081 tgcagaaaga tacgcaggag cttcccgatt tgggcacgac ctggggcgcg cccgttttgc
     7141 ctgccgacgc ggcagcccgt cgcctgcgcg cggaaaccgt ttcgaaatgg acgaacccga
     7201 ataattattt ccgtcgcgga gttcgacttt tcaacgccga gaagctgagc tttacggcgg
     7261 caagcggaaa gctttcgccg accaagggaa tcaccatcgc ttcggaaaat atggtttatc
     7321 tctggggcaa ttacaacgcg accggaatta ccggcattcc gtcgggcggc tcgaccttaa
     7381 acacgggcgg atttaccggc gcgcaagtgc cgtcttcgat tgtctgcg
//
LOCUS       DASWNE010000043         3019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_249022, whole genome shotgun sequence.
ACCESSION   DASWNE010000043 DASWNE010000000
VERSION     DASWNE010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249022"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..1957
                     /locus_tag="VGB68_00930"
     CDS             128..1957
                     /locus_tag="VGB68_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY0047818.1"
                     /translation="MKTTDLFRKKTIEQIQADVDAGMSEHAQDDVGGTGAKLRRTLKL
                     RDLTLMGIAAIIGAGIFAMIGGAAHRGGPAVIFLFAFTAIACGFSALCYSEFASRIPI
                     AGSAYTYAYASFGELAAWIIGWDLIVEYAIGNIAVAISWSDYFTRLLTGYGMKIPAYM
                     TMDYLSASRGFADNQEAVAQALGQGHTLDALSRMKGDDLVAISEKLTTGAIESYEAWT
                     TAPMVGDWRIIADFPALMITFFITILVYIGIRESKMVGNAMTVLKIGIILTVIVLGAF
                     YVAPANWTPFMPNGLGGVMASVAAVFFAYIGFDALSTTAEECENPKRDLPRAMIYSLI
                     ICTVLYIALALVLTGMESYTNLNVGDPLAFVFREKLPWLSGIIAVSAVIALATVFLVF
                     QIGQPRLWMAMSRDGLLPKIFSSIHPRFKTPWFSTIVTGIVVAIPALFMNLTEVTDLG
                     VIGTLFAFTLVCGGVLIKDKDFGSENRFVPYVNSAYIIPPLFIIAMVALFIYNDEAVV
                     RFFTDFSPTEAGETTFGAFSHKIPYLVFIIFALIMTYLSVAKNLSLIPVLGVLSCTYL
                     MSELGIVNWMRFGVWLLVGLIIYFIYGYKNSHLHKGTVGVAEQ"
     gene            2134..2697
                     /locus_tag="VGB68_00935"
     CDS             2134..2697
                     /locus_tag="VGB68_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047819.1"
                     /translation="MKKLAVIAAVLCLFSLSVFAQSKTSFAGNWELDAKKSKLPEMSR
                     VESISMKVTQTDKDLKIESTTKRTPRPESDAGSGAGNRRGNWGGGGFGGDGTQTYNYS
                     LEGKETSTEAVAGIPAASATLKANLEKDGKLSLTSTRKFNSQMGETTVTTRETWELVD
                     SGKTLKLVRETQSPRGTQTSEMYFTKK"
     gene            2799..>3019
                     /locus_tag="VGB68_00940"
     CDS             2799..>3019
                     /locus_tag="VGB68_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047820.1"
                     /translation="MIIAETSFAEAVEEFKKFLAKNNQPTEILWVFLEDTFAGNTTHF
                     EKHLRLKLPLPEENEKLAEKLYGIGQQRK"
BASE COUNT          728 a          714 c          808 g          769 t
ORIGIN      
        1 tttaaatttt tttggaattt ttactaagaa aatactaatt cgacgcaatt tccattgcat
       61 ctggcggaaa attcgttcaa aattcgcaca ccaaagtaat tctactaaat tcaatcaaat
      121 cgaaattatg aaaacaaccg atttatttcg taaaaaaacc atcgagcaga ttcaggcgga
      181 tgtcgacgcc gggatgagcg agcacgccca ggacgacgtc ggcggcacgg gcgcgaaact
      241 acgccgcacg ctgaagctgc gcgatttgac gttaatgggc atcgccgcca tcatcggcgc
      301 cggaattttt gcgatgattg gcggtgcggc gcatcgcggc ggtccagccg ttatttttct
      361 gttcgccttt acggcgattg cgtgcggttt ttcggcgctt tgttattcgg agtttgcctc
      421 gcgcatcccg attgcgggct cggcttacac ttacgcttac gccagtttcg gcgagctggc
      481 ggcgtggatt atcggctggg atttgattgt cgaatacgcc atcggcaaca tcgccgttgc
      541 tatttcgtgg agcgattatt ttacgcgcct gctgacgggt tacggaatga aaattcccgc
      601 ctatatgacg atggattacc tgagcgcttc gcgcggtttc gccgataatc aggaagccgt
      661 cgcgcaggcg ctcggtcagg gtcacacgct cgacgcgctg agccggatga aaggcgacga
      721 cctggtcgcg atttcggaaa aactgacgac cggcgccatc gaatcctatg aagcgtggac
      781 gaccgcgccg atggtcggcg actggcgaat tatcgccgat tttcccgcgc tgatgattac
      841 gttctttatt accatcctgg tttatatcgg catccgcgaa tcgaaaatgg tcggtaacgc
      901 gatgacggtt ttgaaaatcg gaattattct gacggttatc gttttaggag cattttacgt
      961 cgcgccggca aactggacgc cgtttatgcc gaacggcttg ggcggcgtaa tggcaagcgt
     1021 ggcggcggta tttttcgcct atatcggttt tgacgcgctt tcgacgaccg ccgaagaatg
     1081 cgaaaatccg aagcgcgatt tgccgcgcgc gatgatttac tcgctgatta tctgcacggt
     1141 gctttacatt gcgctggcgc tggttttgac gggaatggaa agctacacga acctgaacgt
     1201 cggcgacccg ctggcgtttg ttttccgcga aaaactgccg tggctttccg gtatcatcgc
     1261 cgtcagcgcg gtgattgcgc tggcgaccgt gtttctggtt ttccaaatcg ggcagccgcg
     1321 tttgtggatg gcgatgagcc gcgacgggct tttgccgaaa atattttcct cgattcaccc
     1381 gcgttttaaa acgccgtggt tttcgacgat cgtcaccgga atcgtggtcg ctattcccgc
     1441 cctgtttatg aacctgacgg aagtgacgga tttgggcgtc atcggaacct tgttcgcctt
     1501 tacgctggtc tgcggcggcg ttttgattaa agacaaggat ttcgggagcg aaaatcgctt
     1561 tgtgccgtat gtcaattcgg cgtatatcat tccgccttta tttattatcg cgatggtcgc
     1621 gctgtttatt tacaacgacg aagcggtggt cagattcttt accgattttt cgccgaccga
     1681 ggcgggcgaa acgacgttcg gcgcgttttc gcacaagatt ccgtatctgg tcttcattat
     1741 ttttgctttg ataatgactt atctgtcggt ggcaaaaaat ctttcgctga ttccggtgct
     1801 cggcgttttg tcgtgcactt atctgatgag cgagctcggc atcgtcaact ggatgcggtt
     1861 cggcgtctgg ctgctggtcg gcttgattat ttattttatc tacggctata aaaacagcca
     1921 tctgcacaag ggcacagtcg gcgtcgccga acaatgaaat taagggcaaa agccggaagt
     1981 cagaagataa atttctactg gcttccggct tccgagttcc gattgcactt aatttacgaa
     2041 actttatatt ttcagagtaa ccaaacgctt tgttttcacg tctaacagtg gcggaagaaa
     2101 attcttttct aagattcaca aggagcaatt taaatgaaga agttagctgt tatagcggca
     2161 gttttatgcc tgttttcatt aagcgttttc gcgcagagca aaaccagttt cgccggtaac
     2221 tgggagcttg acgccaaaaa atcgaaactg cccgaaatgt cgcgcgtcga gtcgatttcg
     2281 atgaaagtca cgcagacgga caaggattta aaaatcgagt cgaccactaa aagaacgccg
     2341 cgcccggaaa gcgacgccgg cagcggcgcg ggaaatcgac gcggcaattg gggcggcggc
     2401 ggtttcggcg gcgacggcac gcagacgtat aattacagtc tggaaggcaa agaaacctcg
     2461 accgaagcgg tggcgggaat cccggcggca tcggcgacgc tcaaagccaa tctggaaaaa
     2521 gacggaaagc tgagtctgac ctcgacgcgc aaattcaatt cgcaaatggg cgaaacgacc
     2581 gtcacgaccc gggaaacctg ggaacttgtg gacagcggaa aaactttaaa actcgtgcgc
     2641 gaaacgcagt cgccgcgcgg cacgcagact tcggaaatgt attttacgaa aaaataagtt
     2701 ttaaaaaata agttttactg aacaggtctc atcacagaac tggacgcaag agcttcggct
     2761 cttgcatttt tttgtgctta aattgagtcg ataaatttat gattatcgcc gaaacttcat
     2821 ttgccgaagc agtagaggaa ttcaaaaagt ttctggctaa aaataatcag ccgacggaaa
     2881 ttctttgggt ttttctcgaa gatacgtttg ccggaaacac gactcatttt gaaaagcatc
     2941 tccggctgaa actgccgctg ccggaagaaa acgaaaaact ggcggaaaag ctttacggaa
     3001 tcggtcagca aagaaaatt
//
LOCUS       DASWNE010000044         5697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_36928, whole genome shotgun sequence.
ACCESSION   DASWNE010000044 DASWNE010000000
VERSION     DASWNE010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5697
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36928"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="VGB68_00945"
     CDS             complement(<1..800)
                     /locus_tag="VGB68_00945"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0047821.1"
                     /translation="MLFARRRQFLSAFFAVVLTVAFSAFVFAQDESGEPDPLKIFNQG
                     QDAHEKSDFQTAIKFYNEALKIIPEFPEAEYQRATALLSLNRADEAEKGFRRAIELRR
                     DWTLPLATLGSLLVRKNNFAEAEKLLTRAIELDELSSPAYVALAELRLKTRASETTLK
                     DLLARLQNLSVKAKATASVWAARGAVERALGDKAASKASLGRALSIEPSNSFALSEQI
                     EISLAEGDFTRAVSDAQTLVKSAPNSTSGKLYLARSYAESGNSAEALK"
     gene            1013..1873
                     /gene="larE"
                     /locus_tag="VGB68_00950"
     CDS             1013..1873
                     /gene="larE"
                     /locus_tag="VGB68_00950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HEY0047822.1"
                     /translation="MAVTEEIIAPTASPSTLEKEQRLRRLMREMQSVLVAFSGGVDSS
                     YVATIAAQELGSKAVCVTGISPSLSQIQRETAEKIAADFNFNFIKIQTDELDDPDYRA
                     NPINRCYFCKTELYGKLFSFAAERSINFVLDGSNTDDVSDFRPGREAAREKGVRSPLI
                     EVDMSKAEIRQLSRRQNLPTWDAPSSPCLSSRIAYGIPVSIERLGKIERGEEALRRLG
                     FKEFRVRYHGELARLEIAPAEMERALHKETTDCMADEFRKLGFRYVTLDLHGYRTGAM
                     NEVLEKNLKN"
     gene            1950..3245
                     /locus_tag="VGB68_00955"
     CDS             1950..3245
                     /locus_tag="VGB68_00955"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEY0047823.1"
                     /translation="MATTNPMADEMRRFKGSDEKNPFEAMSERFDRAAEKLGLDPDLY
                     TVMRVPSREIKVYIPVKMDSGHLVVFEGYRVQHNFARGPAKGGIRYAPDVTLDEVKAL
                     SAWMTWKCAVVNVPFGGGKGGIACDPKQMSQGELERMTRRYTAELIDFIGPDKDVPAP
                     DMNTNEQTMAWIMDTYSMHARHTVTAVVTGKPVALGGSLGRREATGRGVQFVVDEAIK
                     RFDLTPEKTTVVVQGSGNVGGIGATLMYEKGYKIIAISDVAGGIYNAKGINIPEALNY
                     LNNTKSFEGYQGAEQVGNAELLEIECDVLAPCATENQITSQNADRINCKILAEGANGP
                     TTPKADKILHDKGIFVIPDILANAGGVTVSYFEWVQDRMGYFWREDEVNERLQDKMVA
                     SFNELCHYATKHDVDTRTAAYMLAIDRVAYDTKMRGIYA"
     gene            3676..>5697
                     /locus_tag="VGB68_00960"
     CDS             3676..>5697
                     /locus_tag="VGB68_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047824.1"
                     /translation="MGGPTGLFTVYDGQTLRRGEFTFSIAYSNFDRDPGNADFVEVPV
                     SFQIGLSDHLELFFNTDAYRAIKVNSPDNLSGFYLPNSRLRRPTTGIGTIQPTGSALV
                     SPAAIILAPRGAGASQFGNAAIFRPQGQQPFLQYPYVGGGVGNFGLFFPSGPIFGFPA
                     GTFPTVGPPTQGGNGADLFPGIGSPVGGILPGLVLQTVCTAPNPTAANCNALNQAPTV
                     FTLAPSYLPDAPFLNRTYGETAFNTFTAGGKWRWTGPSNPVGVGVIAYYRWYADKPDS
                     LSGFNQLQRGASPGGDWGDIGVVLFGDARLKTWLNVSANLGYNYNSSVKGEFPNGEFT
                     LLDRPDEVLAGVAVDFPVNRYFQPILEFRSTQYVGGRTPNAFENSPIEGLAGFRWFPA
                     RWLGMGFAYRHHFNQQDADFIENSDFNGTVTVTGGSPTAPTQTIVTSTGGIRGFVTST
                     DPHGFIIQGFIGRRNKRGVTPPQNLPPNVTGLAFDRTAVTLPCPPGTIPVEGTTCPEP
                     TVRVTTTAVDPENDVLTYNYTVSGGRITGQGANVTWDLSGVRPGTYTITAGVDDGCGI
                     CGKTETRTIEVTECNCVEPPRPCVCPTVSISGPSGVTEPGGTMTFTANVSGGTAENIT
                     YNWTVSAGTIESGQGTPSITVSTTGLVDTNVTATVEIGGTGLCADCPRNL"
BASE COUNT         1400 a         1433 c         1576 g         1288 t
ORIGIN      
        1 atttttaagg cttccgccga atttccgctt tcggcgtacg agcgggcgag atacaatttt
       61 ccgctcgtcg aattcggcgc ggatttgacg agcgtctgag cgtcgctcac ggcgcgcgtg
      121 aaatcgcctt ccgccagcga aatctcgatt tgctcggaaa gggcgaacga attggaaggc
      181 tcaatcgaaa gcgcgcgccc gaggctcgct ttcgacgccg ctttgtcgcc cagagcgcgt
      241 tcgaccgcgc cgcgcgccgc ccagaccgaa gccgtcgctt tcgccttcac ggaaagattt
      301 tgcagtcttg cgagcaaatc tttcagagtg gtttcggacg ccctggtttt taaacgcaat
      361 tcggcgagcg caacataagc cggagagctt aattcgtcca gctcaatcgc tctggtcaaa
      421 agtttttcgg cttcggcgaa attatttttg cgaaccagca gggaaccgag cgtggcgagc
      481 ggcagcgtcc agtcccggcg cagctcgatg gcgcggcgaa aacctttttc ggcttcgtcg
      541 gcgcgattga gcgagagcag agccgtcgcg cgctgatatt cggcttcggg aaattcggga
      601 atgattttca aagcttcgtt gtaaaatttt atcgcggttt gaaaatcgct tttttcgtgc
      661 gcgtcctgtc cctggttgaa aatcttcaac gggtcgggct cgcccgattc gtcctgagca
      721 aaaacgaacg cggaaaaagc gacggtcaga acgacggcga aaaacgcgga caaaaattgc
      781 cggcgacgag caaaaagcat cgaaaaaaat ctcacttcga attttgagtc gggggaagac
      841 atctgcatat taacacgagt ccgccggaag taaaaagtaa aaagtaaaaa ggcgaaaaaa
      901 cgccttaatt attaattttt ctgaaattcg atagttgcac acctcgattc actgcgataa
      961 gattttggtt ttgagatttc gttccgcaaa acaaaagctt tcgcctgaga aaatggcagt
     1021 aaccgaagaa attatcgcgc cgaccgccag cccgtccaca ctggaaaaag aacaaaggct
     1081 ccgccgtttg atgcgcgaga tgcaatcggt gttggtcgcc ttcagcggcg gcgtcgacag
     1141 ctcttatgta gcgacgattg ccgcgcagga actgggctca aaggcggtct gcgtgaccgg
     1201 catctcgccg agcctttcgc aaatccagcg cgaaaccgcc gaaaaaatcg ccgccgattt
     1261 caacttcaat ttcattaaaa tccaaaccga cgagctcgac gaccccgatt accgggcaaa
     1321 tccgattaat cgatgctatt tctgcaaaac cgagctttac ggcaaattat tttcctttgc
     1381 cgcggaaaga agcataaatt ttgttttgga cggttccaat acggatgacg tgtccgattt
     1441 tcgtccgggc agagaagcgg cgcgggagaa aggcgtgcgc agcccgctca tcgaagtaga
     1501 tatgagcaag gcggaaatcc gccagctgag ccgcaggcag aatctgccga cgtgggacgc
     1561 tccgtcgagc ccttgcttat cgtcgcgaat cgcttacggc ataccggttt cgattgaacg
     1621 gctcggcaaa atcgagcgcg gcgaagaggc tttgcgacgg ctcggattta aggaattccg
     1681 cgtccggtat cacggcgagc tggccaggct ggagattgct ccggcggaaa tggaacgtgc
     1741 attgcataag gaaacgaccg attgtatggc ggacgagttc agaaaactcg ggtttcgcta
     1801 tgtaacgctc gatttgcacg gctaccggac tggcgcaatg aacgaagttt tagagaaaaa
     1861 tcttaaaaat tagataaaac tacatagaaa gcagccgatt aaagtttgat taaacggctg
     1921 ccggataatt ttttggagag caaaacaaaa tggcaacgac caatccgatg gcagatgaaa
     1981 tgcggcgttt taagggcagc gacgaaaaaa atcctttcga agcgatgagt gaacgctttg
     2041 accgcgccgc tgagaaactg ggtttagacc ctgatttata cacagtgatg cgtgttccga
     2101 gccgggaaat caaagtttat attccggtta agatggattc gggacacctc gtggtctttg
     2161 aaggctaccg cgtccagcac aatttcgcgc gcggtccggc aaaaggcgga atccgttacg
     2221 cgcccgatgt cacgctcgac gaagtcaagg ctttatcagc ctggatgacc tggaaatgcg
     2281 cggtcgtcaa cgttcctttc ggcggcggta aaggcggaat cgcctgtgac ccgaaacaga
     2341 tgtcgcaggg cgagctggaa cgtatgacgc gtcgctacac cgctgagctg attgatttta
     2401 tcggtcccga taaggacgtc cccgcgccgg atatgaacac caacgagcag acgatggcgt
     2461 ggattatgga cacttattca atgcacgcgc gccacaccgt gacggcggtc gtgacgggta
     2521 agccggtcgc tctgggcggc tcgctcggtc ggcgcgaggc gacggggcgc ggcgttcagt
     2581 ttgtggttga cgaagcgatt aaaagatttg atttaacgcc ggaaaaaacc actgtcgtcg
     2641 tacagggctc gggcaacgtc ggcggaatcg gcgcgacgct gatgtacgaa aaagggtata
     2701 agataatcgc catttccgat gtcgcgggcg gaatttataa cgcgaaaggg attaatattc
     2761 ccgaagcatt aaattattta aataacacga aatcgtttga aggttaccag ggcgcggaac
     2821 aggtcggcaa cgccgaattg ctcgaaatag aatgcgatgt gctcgcgccg tgcgcgacgg
     2881 aaaatcagat tacgtcgcag aatgccgacc gcatcaactg taagatttta gccgagggcg
     2941 ccaacggtcc gaccactccg aaagccgata aaatcctgca cgataaaggg attttcgtga
     3001 ttcctgatat tctggcgaat gccggcggcg tgacggtttc ttattttgaa tgggtgcagg
     3061 accgcatggg ttatttctgg cgcgaagacg aggttaacga gcggcttcag gacaaaatgg
     3121 tggcgagctt taacgagctt tgccattatg cgaccaagca cgatgtcgac acgcgcacag
     3181 ccgcttatat gctggcgatt gaccgcgtcg cttacgacac gaaaatgcgc ggcatttacg
     3241 cctaggctgg aaatgctatt tatttgtatt acaactaaat aaattgcttg attttcaaat
     3301 aaatatgaag taatgtcttt acgaggaagt atttgctgaa aagaaataaa ttattgacat
     3361 ttcggcgata ctaacttata aaaagttttg aaacaaaata gtttcttgta ttactttggc
     3421 tgaaagtatc agtcatagac tgttttcggc agaagcaata atttgctgta gctttacaga
     3481 aaacctactg gaagctacga tttaaaatta aattttaata ttaattttcg gcggaggaat
     3541 gtgatgagac tcgcgaaaag agcgtttata atcgcgctta ccatgttgtc attgctggta
     3601 gcaatgacag cacaaacacc tagatcggag aacgatccca gaaatacggc accgactgtt
     3661 ggtacaggcg gtccgatggg cggtccaacc ggtcttttta ccgtttatga cggacagact
     3721 ttaagacgcg gcgaatttac ttttagtatc gcttacagca actttgaccg tgatccgggc
     3781 aacgcggatt ttgttgaagt gccggtgagc ttccaaatcg gtttgagtga ccatttggaa
     3841 ttgttcttta atacggatgc ctaccgggcg ataaaagtta attctcctga taatctttct
     3901 ggtttttatc ttcccaattc cagactgcgc agaccgacga ccggaatcgg cacgatacag
     3961 ccgaccggca gcgctttggt cagcccggca gctatcattt tagcgccgcg cggggcgggc
     4021 gccagccaat ttggcaacgc ggcgattttc cgtccgcagg gacagcagcc gttcctgcaa
     4081 tatccttatg tcggcggcgg cgtcggcaac ttcggattgt ttttcccgtc gggtccgatt
     4141 ttcggatttc cggcaggcac tttcccgaca gtcggaccgc ccacgcaggg cggcaacggc
     4201 gctgacctgt ttccgggcat cggctcgccg gtcggcggta ttttgccggg cttagttctg
     4261 caaactgttt gtacggcacc gaatccgacc gccgccaact gtaatgcgct gaaccaggct
     4321 ccgacagtgt ttactttagc gccgagctat ctgccggacg ctcctttcct gaaccgcact
     4381 tacggtgaaa ctgcatttaa cactttcacc gccggtggaa aatggcgctg gacgggaccg
     4441 tctaatccgg tcggcgtcgg tgttatcgcc tactatcgct ggtatgcgga caagcccgac
     4501 agccttagcg gattcaacca gcttcagcgc ggcgcaagcc cgggcggaga ctggggcgac
     4561 atcggcgtcg ttttgttcgg cgacgcgcgt ctgaagacgt ggctgaacgt ttcggctaac
     4621 ctcggttaca actacaacag cagcgtcaag ggcgaattcc cgaatggaga atttaccttg
     4681 ttagaccgtc cggacgaagt tctggcgggc gtcgcggttg actttccggt aaacagatat
     4741 ttccagccga ttctggaatt ccgttccact caatacgtcg gcggacgcac gccgaacgct
     4801 tttgaaaaca gtccgattga agggctggct ggtttccgct ggtttccggc tcgctggtta
     4861 ggtatgggct ttgcctatcg ccaccacttc aatcagcagg atgcggactt tattgaaaat
     4921 tcagacttca atggaaccgt tacggtaacc ggaggctcgc cgaccgcgcc gacgcagaca
     4981 atcgtcacca gcaccggcgg catcagaggc tttgtgactt cgaccgaccc gcacggattt
     5041 attatccagg gctttatcgg tcgtcgaaac aaacgcggcg taactccgcc gcaaaactta
     5101 ccgccgaacg taacgggact cgcgtttgac agaactgcgg ttactttacc gtgccctccg
     5161 ggaacgattc cggttgaagg aacgacctgt ccggagccga ccgtcagagt aacgacgacg
     5221 gcagtcgacc cggaaaacga tgtgctgacc tataactaca cggtttcggg cggacgcatt
     5281 acgggtcagg gcgcaaacgt gacctgggat ttgtccggcg tcagaccggg aacttatacg
     5341 attaccgccg gcgtcgacga tggctgcggc atctgcggca agacggaaac cagaacaatc
     5401 gaagttaccg aatgtaattg tgttgaaccg ccgcgtccgt gcgtctgtcc gacagtttcg
     5461 attagcggac cgtcaggcgt aaccgaaccg ggtggaacga tgacctttac ggctaatgtc
     5521 agcggcggca ctgccgaaaa cattacctat aactggactg tttccgccgg aacgattgaa
     5581 agcggtcagg gaacgccgag tattacagtc agcacgactg gcttagtaga tacgaacgtc
     5641 acggcgacgg ttgaaatcgg cggaacggga ctttgcgcgg actgtccgag aaacctg
//
LOCUS       DASWNE010000045         1565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_567056, whole genome shotgun sequence.
ACCESSION   DASWNE010000045 DASWNE010000000
VERSION     DASWNE010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567056"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VGB68_00965"
     CDS             <1..299
                     /locus_tag="VGB68_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047825.1"
                     /translation="QWFDEGNKLLLSETASAEECVYLLESVPELIDSTLVPLEFGANE
                     QAKLVSACEFVLEGLYAQNKISRNEEGGFEATTKAKRDRRGMIYEDFTDIEGYN"
     gene            384..1496
                     /locus_tag="VGB68_00970"
     CDS             384..1496
                     /locus_tag="VGB68_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047826.1"
                     /translation="MTTYTFLYTAANPDGKNVTERIEAENIGQAKYRLEIRGYSEITF
                     HANEMRDEVCQTFDVKENPLNKVSPQIENKLKTQGVVEGVLFSLKAWAIFWIPLLAWL
                     IYDIAKGESFWLSLALFVAFVFYFIFINLPSVFYKLLIEAYDWARWNEVRSRVKIIRW
                     YNKISPLAVPEFDLESRLACADAAEGNLENALRRMVKYENHPKVSRFLYYARMAVIYE
                     KAKRPDKKLEYQELTIREYPERTEGYIDCAFTQARYFKNTAKAREILDAATEREITFI
                     AQPFVSFCRGIIELEDGNLQKAEFDLLRALEKTKPFEKAQLFTGVISELKAYLSLLYG
                     QIGEKDKAFDFLQEAKPYLLANNEDELLERCEKALA"
BASE COUNT          461 a          330 c          400 g          374 t
ORIGIN      
        1 tgcaatggtt tgacgaaggc aacaagcttt tattgagcga aacggcttcg gcggaagaat
       61 gcgtgtattt gctggaaagc gttcccgaat taatcgactc gacgctcgtc ccgctcgaat
      121 tcggagcaaa cgaacaggcg aagctcgtct cggcgtgcga attcgttctg gaaggactct
      181 acgcgcaaaa caaaatctcg cgcaacgagg aaggcggttt tgaagcgacg acgaaagcca
      241 agcgcgaccg gcgcggaatg atttacgagg attttaccga catcgagggc tataactaaa
      301 aatctggaaa ccctgcggca atagagattt tctaatatta gagacgttcc gtctcttcta
      361 agttctgcaa aggaaaatct cgaatgacaa cttatacttt tctttacacg gcggcgaatc
      421 cggacggaaa aaatgtcacc gaaagaatcg aagccgaaaa tatcggtcag gcaaaatatc
      481 gtctggaaat cagaggctat tcggaaataa cctttcacgc caatgaaatg cgcgacgaag
      541 tctgtcagac gtttgacgta aaggaaaacc ctttaaataa agtcagcccg caaatagaaa
      601 ataagctcaa aacgcagggc gtcgtcgagg gcgttttatt ttcgttaaag gcttgggcaa
      661 tcttctggat tcctttgctg gcgtggctga tttacgacat cgccaaaggc gaatccttct
      721 ggctgagcct ggcgcttttc gtcgcctttg ttttctactt catttttata aatctgccga
      781 gcgtttttta caaattgctt atcgaagcct atgactgggc gcgttggaac gaagtcagaa
      841 gccgggtaaa aatcatcagg tggtataaca aaatcagccc gctggctgtt cccgaattcg
      901 atttggaaag tcgtctggcg tgtgcggacg cggcggaagg gaatctggaa aacgccctgc
      961 ggcgaatggt gaaatacgaa aatcacccga aagtgtcgag atttttatat tacgccagga
     1021 tggcggtaat ttacgaaaaa gccaaaagac ccgataaaaa attagaatac caggaactga
     1081 ccatcaggga atatccggag cgaacggaag gctacatcga ctgcgccttt acacaggcga
     1141 gatatttcaa aaacacggcg aaggcgcgcg agattctgga tgcggcgacg gaaagagaga
     1201 taacgtttat cgcgcagccg ttcgtttctt tctgccgggg aattatcgaa ctggaagacg
     1261 gaaatttgca gaaagccgaa ttcgacttgc tgagagcttt ggaaaaaacc aagccgtttg
     1321 aaaaggcaca gctttttaca ggcgtcatca gcgaattaaa agcatattta tctttacttt
     1381 acgggcaaat cggagaaaag gataaagcgt ttgatttttt acaggaagcg aagccttatt
     1441 tgctggctaa taatgaggac gagctgcttg agcgctgcga aaaagcgttg gcttgattaa
     1501 atctttttag ctgaaatgaa aaaagccgcg ccgaagtttt gcaaaacttc ggcgcggctt
     1561 ttttc
//
LOCUS       DASWNE010000046         4006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_1804, whole genome shotgun sequence.
ACCESSION   DASWNE010000046 DASWNE010000000
VERSION     DASWNE010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1804"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..356)
                     /locus_tag="VGB68_00975"
     CDS             complement(78..356)
                     /locus_tag="VGB68_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047827.1"
                     /translation="MSEITTPIAVINEIFGKDYSASREETKKKYGEQAEADAKKVYEA
                     VMAIPWYGSASYDNNKSSFSADCERLFPELSEEAISNLHWSYAASWIP"
     gene            1120..1629
                     /locus_tag="VGB68_00980"
     CDS             1120..1629
                     /locus_tag="VGB68_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047828.1"
                     /translation="MKRKLLIGIVTVMVFAFGVGNVAAQSDFSGVWTLDAGKSANVPS
                     GLMQTMTVTQKGDRVEIETKSKAGEGQERVGKDTYVLDGKETDFNMGRGAKNAKRISK
                     LTADGNGFDVTEEFVVNQPDGGEATVRANRRWLLSADGKTLTIEMVAENPNDASKPRS
                     RSRRVFVKK"
     gene            1714..2328
                     /locus_tag="VGB68_00985"
     CDS             1714..2328
                     /locus_tag="VGB68_00985"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEY0047829.1"
                     /translation="MRRKSISLVVILALLAFVSTANACSAGNIADAKGDFAVGARLEN
                     FTLPDLSGAEQSFDKLKGKNGAVLVWVSAQCPVVKSYNERINQIADELKAKGINLIGI
                     NSNATESLDYAASGAKEAGYKFPVLIDKGNVLADKLGASVTPEFFYFDRENVLLYHGA
                     LDNDRSGKNVTINYLRDAFDSALNKQPIKTSKTNAFGCSIKRAS"
     gene            2332..2946
                     /locus_tag="VGB68_00990"
     CDS             2332..2946
                     /locus_tag="VGB68_00990"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEY0047830.1"
                     /translation="MRILSLTKQTRRGAENKFFRAVFFISLCTFFSFVNSCAQTKNPE
                     QKSPAETNSKTKSRAANPPRVTQVDDGKIKQLLKPVGKPLLVNFWATWCGPCREEFPD
                     LVKIDVEYRGKIDFFTVSLDFIEELNTGVPKFLSEMKAEMPTYLLVSADENTLISSIA
                     KDWSGALPFTILYNEKGEIIYLRQGKVNQEQLKGEIEKTLGEVK"
     gene            3513..>4006
                     /locus_tag="VGB68_00995"
     CDS             3513..>4006
                     /locus_tag="VGB68_00995"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY0047831.1"
                     /translation="MAFTLRITIARWALDLPMYCFLLSSIYVVDFYRRFQAEQLKSSE
                     LKAALATSQLNALKMQIHPHFLFNTLNSVSALIHEDQKAADKMVARLGDFLRLTLESS
                     GDHEVSLEQEMDFVGHYLEIESVRFQDRLVVRMNIDPETLAARVPNLILQPIVENAIK
                     HGIS"
BASE COUNT         1146 a          883 c          904 g         1073 t
ORIGIN      
        1 ttttttttat gcaaatgaaa aaagcagtac ttcgaggctt ttctttaatt attaaatttt
       61 gcggcggcgc atcttgatca gggaatccaa ctggcggcgt aagaccaatg caaattacta
      121 atcgcttctt cggaaagctc cgggaacagg cgttcgcaat cagccgaaaa tgagctttta
      181 ttgttgtcgt atgacgccga gccgtaccac ggtatcgcca taaccgcttc ataaaccttt
      241 ttagcgtcgg cttcggcttg ctcgccgtat tttttctttg tttcctcacg gctggcagag
      301 taatcctttc cgaaaatttc attgattacc gctatcggag tagttatttc actcataatt
      361 ttattgattt taagcttatc gtgccaatta tataattaaa acactgttcg gaattctatt
      421 atcctatttt acatataaac aacgcaacaa attgtgagaa tgtttgaaag gcttgattct
      481 attggaggcg gcgctcggaa tcgagccgat gaataaagct tttgccggaa taattaacct
      541 ctgttcactg aagaatacag aagcacaagc agttacacag caaacaatta agtacctggt
      601 ttgttcttcc gtgtgcttca gcgagtgcag gtgattacta caattttgag tctatgctct
      661 tgatttgcgt ccgataaaat atttattaat tacaatcatt ttgtttcaat aaaaaacata
      721 aataaaagac attttaatag cgaaagataa caaaaatcag ctgttcaaaa acctcataaa
      781 ttttcattta taaaacgttc tgagaaattc caatctataa attaatttcg ccctgcacaa
      841 aaagagtagt tttgggacat ccagccccat ttcaaaaagt atatttcccc aaaggaaatt
      901 atgaaatgaa tcttctcgtc gaacactcgg attttcatac gccatcccat taatcccatc
      961 ttgcccttaa tccgccactt tgctgtaccg attcgtcaca aacctgttca aaggcttttt
     1021 agtgacatat atcattagct gaaacctacg gaattaatgc ttattgaaag caagggtttt
     1081 taagttacta aaaaaataaa ccaaatggag aattaagaaa tgaaacgcaa attattaatt
     1141 ggaattgtca ctgtcatggt tttcgctttc ggcgtcggaa atgtcgccgc ccaatccgat
     1201 ttcagcggcg tctggacgct cgatgcaggc aaaagcgcaa acgtgccgtc gggattaatg
     1261 caaactatga ccgtcacgca aaaaggtgat cgtgttgaga tagaaacaaa gtccaaagcc
     1321 ggagagggtc aagagcgcgt cggcaaagac acttacgttc tggacggcaa agaaaccgat
     1381 tttaatatgg gacgcggcgc aaaaaacgcg aaaagaatct cgaagttgac ggctgacggg
     1441 aatggctttg atgtgacaga agaattcgtc gtgaaccagc ctgacggcgg cgaagcaacg
     1501 gttcgagcca accggcgctg gttactttcc gcggacggaa aaacactgac tatcgaaatg
     1561 gttgcggaaa atccgaacga cgcgtctaaa cccaggtcca gatccaggcg cgttttcgtc
     1621 aaaaaataaa atgacgaagg ggctgccgat gatttgccgc ccggttcttt tcaaggcagc
     1681 ccctgttaac tgaataaaaa ggagcaaatt tctatgagac gaaaaagcat ttctctggtc
     1741 gtgattcttg cgcttctggc atttgtttca acagcaaacg cctgcagcgc gggaaatatt
     1801 gctgacgcga aaggtgattt tgccgtcggc gccaggctgg aaaattttac tttgccggat
     1861 ttgagcggcg ccgagcagtc gtttgacaaa ttaaagggca aaaacggcgc ggttcttgtt
     1921 tgggtttcgg cgcagtgccc ggtcgttaaa tcctacaacg aaagaattaa ccagattgcc
     1981 gatgaattaa aagccaaagg catcaacctg atcggcatca attcaaatgc gaccgaatct
     2041 ttggattatg ccgcatcagg cgctaaggaa gccggttaca aattcccggt tttgatcgat
     2101 aaaggaaacg ttttggcgga caagctcggt gcgagcgtca cgccggagtt tttctatttt
     2161 gacagggaaa acgttttgct ttatcacggc gcgctcgaca acgaccgttc gggaaaaaac
     2221 gtgacgatca attacctgcg cgacgctttt gattcggcgc tcaataaaca accgatcaaa
     2281 acgagcaaaa caaacgcttt cggctgctcg atcaagcgcg caagctaaat catgagaatt
     2341 ttaagtttaa ccaaacagac gcggcgcggc gcggagaata aatttttccg cgccgtattt
     2401 ttcataagtc tctgcacgtt tttctccttt gtaaactcgt gcgcacagac aaaaaatccc
     2461 gaacaaaaat cgcctgccga aacgaattcc aaaactaaat cgcgggcggc aaatccgccg
     2521 cgcgtgacgc aagttgacga cgggaaaatc aaacagcttc tcaaacccgt cggcaagcca
     2581 ctgctcgtta atttctgggc gacgtggtgc ggtccgtgcc gcgaagaatt tcccgacctt
     2641 gtcaaaatcg acgtcgaata tcgcggaaaa atcgactttt tcaccgtttc gctggatttt
     2701 atcgaagaat taaacacggg agtgccgaaa tttttgagcg agatgaaagc ggaaatgcca
     2761 acctatttgc tcgtcagcgc agatgaaaac acgctcatct cctcgattgc caaagattgg
     2821 agcggcgctc tgccgttcac gattctttac aacgaaaaag gcgagataat ctatctccgg
     2881 cagggaaagg taaaccagga gcaattgaag ggcgaaatcg aaaaaacatt gggcgaagtg
     2941 aaatgaggcg aggcgaaaac gttattttta acttttctca cgatttattt atgtattaac
     3001 tgtgcgaaaa acacacagag agcatagcgg gtaataaaaa aacagacgca gctacgaaag
     3061 acttttgggg aattattagc taaagttagt gaaattagta aaataaaacg gcggtgtcga
     3121 agttcgaccg ctatttgttt tgtaaggata tcaatgaaat tttgatgcga aagttcaatt
     3181 tgcagcaaat actaattttt tcggcgggct ggattttgct ggcatttctc ttttcgggtc
     3241 tgacctattt ttccaacgct caaggaaacc gcccccgacc attcgaatgg aaaaacgtct
     3301 ttttatcgca agcgataatt tacagctggg caattctctc gccgctaatc gttttcttcg
     3361 ccaagcgatt ccgcttcgag cagcgaaatt ggtggcgtct gttacccgca catcttgttg
     3421 cggcggtagt ttttctactg cttcacacag ctattcattt catcttcgtt aaaattgttg
     3481 atccggcgcc agttgccgag agcggtgggt ttttggcttt tacattaagg ataaccattg
     3541 caaggtgggc tctggatttg ccgatgtatt gttttctgtt gagcagcatt tacgtggtcg
     3601 atttttaccg gcgttttcaa gccgaacagt taaaatcttc ggagctgaaa gccgcccttg
     3661 ccacctcgca gcttaatgcc ttgaaaatgc agattcatcc gcattttctg ttcaacactt
     3721 taaattcagt ttccgccttg attcacgaag atcaaaaagc agcagataaa atggtcgccc
     3781 ggctcggcga ttttctgcgc ttgacgttgg agagttccgg cgaccatgaa gtttcgctcg
     3841 aacaggagat ggactttgtc gggcactatc tggaaatcga aagcgtgcgg tttcaagacc
     3901 ggctcgtcgt caggatgaac atcgatccgg aaacgctcgc ggcgcgagtt cctaatttga
     3961 ttctccagcc gattgtcgag aacgccatta aacacggcat ttctca
//
LOCUS       DASWNE010000047         6946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_143263, whole genome shotgun sequence.
ACCESSION   DASWNE010000047 DASWNE010000000
VERSION     DASWNE010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6946
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143263"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VGB68_01000"
     CDS             complement(<1..380)
                     /locus_tag="VGB68_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047832.1"
                     /translation="MNSAKLIIFFLLLFSFSSVYAQRDKPGTQAELDAITARGRALYE
                     YDQAAWHSTDAVFALAPAEGSFTMYVGKKTDKGWTVAYGKLSDKKDKYLIVYEAAQGA
                     TAKEFKVTKFDKPKEDTGFYLSGA"
     gene            complement(408..2306)
                     /locus_tag="VGB68_01005"
     CDS             complement(408..2306)
                     /locus_tag="VGB68_01005"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEY0047833.1"
                     /translation="MRNFRELLDGSGIHVFDGAMGTRLYDKGVYINRSYDELNLTAPD
                     LVREVHEEYVRAGAEIIETNTYGASRHKLQQYSLENQLKEMNLSGARLAKAAAGDRAF
                     VAGAIGPLGLRIEPYGPTSFDEAKEMFAEQISALLEGGVDLFVMETFSDLSEIQQAIR
                     AVKELCDLPVVAQMTIQTDGKTVFGADAEIITQRLEEYGADVIGLNCGVGPTHILSAL
                     EKMREVTTRKLSAQPNAGLPRDVQGRQFYMCSPEYMSKFAKRFIKAGATFIGGCCGTT
                     PKHIKLISDAVRAVSPRSQKFFAKAPEHPAHVKDFTPPDVQVVPAEQRSKWARKIARG
                     EFVTSVEVLPPKGCDAAKTLESIKLLVKAGVDAVNIPDGPRAQTRMSAQATAVLVERE
                     VGIEAVLHYCCRDRNLLGMMSDLLGAAALGLHNLLIITGDPPKMGPYPDATAVFDIDS
                     IGLTNMVSKLNHGLDLGNNPIGCPTAFSIGVGVNPGAINLEEEIRRFEWKVEAGAEYA
                     ITQPVYDTEQLRSFLKKIEHVRIPIVAGIFPLVSIRNAEFMHNEVPGVNVTPEILGRM
                     RKASDISKEAAREEGIKIARESLAEVRDLIQGVQVSAPFGNVKYALQVFDVLDEFKNK
                     RIEAAIAT"
     gene            complement(2616..3989)
                     /locus_tag="VGB68_01010"
     CDS             complement(2616..3989)
                     /locus_tag="VGB68_01010"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY0047834.1"
                     /translation="MKKNLLLSFCSLLLASAFLLSGAEKISAQEPDSKKVSGETRKHR
                     EQTISILREMKGVLKKHYYDANYRGINLEERFAAAEARIKTLEYNWQMYRVLVQVLMD
                     FNDSHTVFHLPPRSDYFDYGFSMQMFGTDCFITAVKKDSDAEKQGLKVGDQLLNIGKF
                     KPTREDLWKIVYVLYRLDPKETVDLTIAKLDGSQQKITVKAKTMTQKEKREELRKRKT
                     KEKFEPFKCQEINAATIACKLTTFMVGKEEIDKMMKRVGNHSKMILDLRGNGGGLVDT
                     EVYLLGYFFDRDVKVADLITREKTETRFARSRGDRAYKGDLVVLTDSRSASASEMTAR
                     VLQLEKRAKIVGDVSMGAVVTSYTVPLFGSYSALSDGVITSVSMGVTIADVKMKDGSR
                     LENIGVVPDYPVIPTGEALSKKMDAVLAYAASLLGAELSPEKAGEFYFITSREIDEDL
                     SAEEGDK"
     gene            complement(4102..4788)
                     /locus_tag="VGB68_01015"
     CDS             complement(4102..4788)
                     /locus_tag="VGB68_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047835.1"
                     /translation="MFLKKLSLTFLALACLIFFSNDAFACACCSETGDYRIWTGKPDQ
                     YSVDLLEEMKFDQAAFLYMNEAGFDALKGLDSLAKAYESDSWVSSPGYFSLTNAFAAR
                     TWKFNFKTKGGKNGALTLPMPAQMVQFKADIHDGKTSGGGGPLLYKEWRYKGVVQTGS
                     GFFQAGIVKPTSYFLVLQGRGNSCDTAADFTAWRLEIEGRKASYAFFGKLSSGQANQS
                     DDSEQSTEKN"
     gene            complement(4854..6056)
                     /locus_tag="VGB68_01020"
     CDS             complement(4854..6056)
                     /locus_tag="VGB68_01020"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY0047836.1"
                     /translation="MAFGILNVTKDNIKDNYYDTNFRGINIDELFAQSKEKLKNASTR
                     DEAMTIIAQTVLMFDDSHTAFYPPSRSADIDYGWLISMIGKDCYVVGVKPGSDAEAKG
                     LKVGDKVLAVDGWRPTQENLWKMYYRYYSIMPTSRVRFVVQSPKDESPRTIDVATKIS
                     KTSKVVTFEDIFNRIIRKGWDVGADRTFESGKDLFVWKMSTFNTSEAHIDAVMDKARG
                     FNSLIIDLRNNGGGSVAVLKRLAGYFSDKDITLCDEKTRKSTKPIIAKTRGNGIYKGK
                     LIVLVDSNSASASEIFARTIQLQGRGKVVGDKTAGAVMESRFFTSKSGVGSVAYFGVS
                     VTIADLIMPDGKSLEKVGVIPDEVVLTKDEDLAEQKDKALSYAAKELGVELSPEKAGA
                     LFPIKWNF"
     gene            complement(6313..>6946)
                     /locus_tag="VGB68_01025"
     CDS             complement(6313..>6946)
                     /locus_tag="VGB68_01025"
                     /inference="COORDINATES: protein motif:HMM:NF018533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEY0047837.1"
                     /translation="GNTLITHLRMTGRFMILPLEREPPKYTHAVLYFADENRLIFQDQ
                     RHFGLMKIVETAFLHETKELKCLAPEPFSDAFTPQYLRAALKTSKRSLKEFLLDQTKV
                     CGLGNIYASEAMFLARANPQTPANEVSARKAGVLFEKIREILAESIAHGSTLNIDPEN
                     IDGSYYDGGYSKGWRVYDREKAPCPNCETPISRLKQGGRSSYFCPKCQRK"
BASE COUNT         1564 a         1841 c         1752 g         1789 t
ORIGIN      
        1 ttcgcgccgc ttagataaaa gccggtgtct tctttcggct tgtcgaattt cgttacctta
       61 aattctttcg cggtcgcgcc ctgcgcagct tcgtaaacaa tcaggtattt gtcctttttg
      121 tcgctcaatt taccgtaagc gaccgtccag cctttatcgg tttttttgcc gacatacatc
      181 gtaaaactgc cttcggcggg cgcaagcgcg aaaaccgcgt cggtcgaatg ccaagccgcc
      241 tggtcgtatt cgtaaagcgc tctgccgcgc gccgtgatgg cgtccagttc ggcttgcgtg
      301 cccggcttat cccgctgcgc gtaaacgctc gagaaggaaa aaagcagcag gaaaaagatt
      361 atcagttttg cggagttcat aattttagta gatgaagcgc agttaaatta agttgcgatt
      421 gccgcttcaa tccgtttatt tttaaattca tccagcacgt cgaaaacctg aagcgcgtat
      481 ttgacgttgc cgaacggcgc ggaaacctgc acgccctgaa tcaaatcgcg gacttcggcg
      541 agagattcgc gggcgatttt gatgccttcc tcgcgcgccg cttccttcga gatgtcggac
      601 gctttgcgca ttctgcccag aatctccggc gtgacgttga cgcccggaac ctcgttgtgc
      661 ataaactcgg catttctgat agagacgagc ggaaaaattc ccgcgacaat cgggatgcgc
      721 acgtgctcga tttttttcag aaacgagcgt aattgttccg tgtcgtaaac cggctgcgtg
      781 atggcgtatt ccgcgcccgc ttcgaccttc cattcaaaac ggcggatttc ttcttccaga
      841 tttatcgcgc ccggattgac gccgacgccg atggaaaaag cggtcgggca gccgatcggg
      901 ttgttgccca gatcaagccc gtgattgagc ttgctgacca tattcgtcaa gccgatggaa
      961 tcgatgtcga aaacggcggt ggcgtccgga taaggtccca ttttcggcgg atcgcccgtg
     1021 atgattagga gattatgaag cccgagcgcc gccgcgccga gcaggtccga catcattccg
     1081 agcagattgc ggtcgcggca gcagtaatgc aggacggctt cgatgccgac ctcgcgctcg
     1141 actaaaacgg cggtcgcctg cgcgctcatc cgggtctgcg cgcgcggtcc gtccggaatg
     1201 ttgacggcgt cgactccggc tttgaccagc agttttatag attccagagt tttcgccgcg
     1261 tcgcagcctt tcggcggcaa aacttcgacc gatgtgacga attcgccgcg cgcgattttc
     1321 cgagcccatt tcgagcgctg ctcggcgggc acgacctgaa cgtcgggcgg cgtaaaatct
     1381 ttgacgtgcg ccggatgctc cggggctttg gcaaaaaatt tctgcgagcg cgggctgacg
     1441 gcgcggacag cgtcggatat taatttaatg tgcttcggcg tcgtgccgca gcagccgccg
     1501 ataaacgtcg cgcccgcttt gataaagcgc ttggcgaatt tcgacatata ttccggcgag
     1561 cacatataaa actggcgtcc ctgaacgtcg cgcggaagtc cggcgttcgg ctgcgccgag
     1621 agctttcgcg tcgtcacctc gcgcattttt tcgagcgccg aaagaatatg cgtcggaccg
     1681 acgccgcagt ttaaaccgat aacgtccgcg ccgtattctt cgagccgctg cgtgatgatt
     1741 tcggcgtccg cgccgaaaac ggttttgccg tcggtctgaa tcgtcatctg cgcgacgacc
     1801 ggcaaatcgc aaagctcttt gaccgcgcgg attgcctgct ggatttccga taaatccgag
     1861 aaggtctcca tcacgaaaag atcgacgccg ccttcgagca gcgccgagat ttgttcggcg
     1921 aacatttctt tggcttcgtc aaaagatgtc ggaccgtacg gctcgatgcg caagccgagc
     1981 ggaccgattg cgccggcgac aaaagcgcgg tctccggcgg cggctttagc caggcgcgcg
     2041 cccgagaggt tcatttcctt taattgattt tccaagctgt attgctgcaa tttatggcgc
     2101 gacgcgccgt aagtgttcgt ctcgataatc tcggcgccgg cgcgcacgta ttcttcgtgc
     2161 acttcgcgga cgaggtcggg cgcggtcagg ttcagctcgt catagctgcg attgatgtaa
     2221 acgcctttgt cgtaaagccg cgtccccatc gcgccgtcga aaacgtgaat gccgctgccg
     2281 tctaaaagtt ctctgaaatt tctcataaat tcaagattcg ggattcggga ttcaggattc
     2341 aggattgaaa gctgacagtc ctgaatcctg aatcctgaat cctgaattaa aaaaaccctg
     2401 cgctaaacaa cgcaaggctt ttttctttaa aagctgttag ctgtttagcg gtttatttta
     2461 cgtggtcgca agttgctcta actcaccacg ccgaaacggt aaaacaaata atgcgatttt
     2521 cagccgcaag tgtcaagttt cgaaaaattc aaaacggtaa acggtcgtaa gttcgaccct
     2581 ttcatttacg accgaccgtt taccgcttgt ttacattatt tatcgccttc ttcagcgctg
     2641 aggtcttcgt caatctccct cgaggtaata aaataaaatt cacccgcttt ttccggcgaa
     2701 agctcggcgc cgagcagcga cgcggcatag gcgagcaccg cgtccatctt tttggacaaa
     2761 gcctcgccgg tcggaataac cggataatca ggcacgacgc cgatattttc cagacggctg
     2821 ccgtctttca ttttaacatc ggcgatggtg acgcccatcg agaccgaggt gatgacgccg
     2881 tccgacaaag cactatagga accgaacagc ggaacggtgt agctggtgac gaccgcgccc
     2941 atactgacgt cgccgacgat tttcgcgcgt ttttccagtt gcagaacgcg cgccgtcatt
     3001 tccgaagcgg aagccgagcg gctgtccgtc aaaacgacca aatcgccttt gtaagccctg
     3061 tcgccgcggc ttctggcgaa gcgcgtttcg gttttctcgc gcgttatcaa atcggcgacc
     3121 ttcacatcgc ggtcgaaaaa gtagccaagt aaataaactt ccgtatcgac cagaccgccg
     3181 ccgttgccgc gcaagtcgag aatcatcttg gaatgattgc cgacccgctt catcatttta
     3241 tcaatctcct ctttgccgac cataaaagtc gtgagcttgc aggcgatcgt cgcggcgttg
     3301 atttcctgac acttgaaagg ctcaaatttt tccttggtct tccttttcct gagctcttcg
     3361 cgcttttcct tttgcgtcat cgtcttggct ttgacggtta ttttctgctg cgagccgtcg
     3421 agcttggcga tggtcaaatc gaccgtctcc ttcgggtcga gccggtataa aacatagacg
     3481 attttccaga ggtcttcgcg cgtcggctta aatttgccga tgttcaaaag ctggtcgccg
     3541 accttcagcc cttgtttttc ggcgtcgctg tcttttttga ccgccgtgat aaaacaatcg
     3601 gttccgaaca tctgcatcga aaacccgtag tcgaaataat ccgagcgcgg cggcaggtga
     3661 aagaccgtgt gcgaatcgtt aaagtccatc aaaacctgca ccagcacgcg atacatctgc
     3721 cagttgtatt ccagcgtttt gattctcgcc tcggcggcgg caaatcgctc ttcgagatta
     3781 atgccgcgat aattcgcgtc gtaatagtgc ttctttaaaa cgcctttcat ttcgcgcagg
     3841 atgctgatcg tctgttcgcg gtgtttcctg gtctcgccgc tgaccttttt cgaatccggc
     3901 tcctgtgcgg agattttttc cgcgccggac aaaaggaaag cgctcgctaa aagcagggag
     3961 caaaaagata aaagtagatt ttttttcata taaatttatt tgccggacag aaggtcgggc
     4021 agattgaggg aacccgaaac gattgaaatt cgccgttttc ggattcatca aaaaatagta
     4081 aaaatagttt ttattgatgt ttcagttttt ttccgtggac tgttcggaat cgtcgctttg
     4141 attcgcttgc cccgaactta gtttgccgaa aaaagcataa ctggcttttc tgccttcgat
     4201 ttccagccgc cacgcggtaa aatccgcggc ggtgtcgcag ctgtttccgc gtccctgcaa
     4261 aacgagaaaa taacttgtcg gcttgacgat gccggcttgg aaaaatccgc ttcccgtttg
     4321 cacgacgcct ttgtagcgcc attctttata aagcagcgga ccgccgccgc cgctcgtttt
     4381 cccgtcgtga atatcggctt taaactgcac catctgcgcc ggcatcggga gcgtcagcgc
     4441 gccgtttttt ccgcccttgg ttttaaagtt gaatttccac gttcgggcgg cgaaagcatt
     4501 ggtcaagctg aaatatccgg gcgaggagac ccacgaatcc gattcgtaag ctttggcgag
     4561 gctgtcgagc cctttcaagg cgtcgaagcc ggcttcgttc atatacaaaa aagccgcctg
     4621 gtcgaatttc atttcttcga gcaaatcgac gctgtattgg tcgggcttgc ccgtccagat
     4681 gcgataatcg cccgtctcgg aacagcaggc gcaggcaaaa gcgtcgttcg agaaaaatat
     4741 cagacaggcg agtgccaaaa acgtcagcga aagttttttc agaaacattt gattttctcc
     4801 gggtttaatt aaaatgcgtt tttaacgccg aaccgtcagg ctttgacgac cgatcagaaa
     4861 ttccatttga tggggaaaag cgctcccgct ttttccggcg aaagctcgac tcccagttct
     4921 ttcgccgcat aagacaaagc cttgtctttc tgctccgcca aatcctcgtc tttcgtcaat
     4981 actacttcgt cgggaatgac gccgactttc tccagacttt tcccgtcggg cattattaaa
     5041 tcggctatcg tcacgctgac gccgaaataa gcgacgcttc cgacgccgga cttggaagta
     5101 aaaaatctcg attccatcac cgcgcccgcg gttttatcgc cgacgacttt tccgcgtccc
     5161 tgcaattgaa tcgtgcgcgc gaaaatttcg gaagccgaag ccgagttgga atcgacgagg
     5221 acaatcaatt tccctttata aatgccgttt ccgcgagttt tggcaattat cggcttggtg
     5281 ctttttcggg ttttctcgtc gcacagcgta atgtctttat cggaaaaata gcccgccaga
     5341 cgcttgagca cggcgacgct tccgccgccg ttgtttcgca aatcgattat cagcgaatta
     5401 aagcctctgg ctttatccat cacggcgtcg atatgcgctt cggaagtgtt gaaagtcgac
     5461 attttccaga caaacaaatc ttttcccgat tcaaaagtcc ggtcggcgcc cacatcccag
     5521 ccttttctga ttattcgatt gaaaatatcc tcgaaagtga cgactttcga agttttcgag
     5581 attttagtcg ccacgtcaat cgtccgcgga ctttcatctt tcgggctttg gacgacaaag
     5641 cgaacgcggc tggtcggcat tatcgaataa taccggtaat acattttcca gagattttcc
     5701 tgcgtcggtc tccagccgtc gacggctaaa actttatcgc cgacttttaa tccctttgct
     5761 tcggcgtcgc tcccgggttt aaccccgacg acgtaacagt cctttccaat catcgaaatc
     5821 aaccagccgt aatcaatatc cgccgagcgc gacggaggat aaaaagccgt gtgcgaatcg
     5881 tcaaacatca gaacggtttg ggcgataatc gtcatcgctt cgtcgcgggt agaagcattt
     5941 ttcagttttt ctttcgactg agcgaaaagc tcgtcgatat taattccgcg aaaattggtg
     6001 tcgtaataat tatctttaat attatctttt gtgacgttca gtatgccgaa agccatatcg
     6061 cgctcgttgc cggcgagttg cgcccgtgta tgaaccgagg cgagggaaat aaacaaaaag
     6121 aatgtcgaca gcgaaataaa aaaagaggtt ttgttttttt tcatataatt ttaatgaaga
     6181 gcttccggaa tcgtttgagt tcggataaaa agtatcggaa ttttttatta aagtaaagaa
     6241 ccgtatcaat cggcgcagta ttttttaaca aaaattccga catctgaaag cagcgccatt
     6301 tgtcctgaag agctatttgc gctggcattt cgggcaaaaa tagcttgagc gcccgccttg
     6361 ttttaaacgc gaaatcggcg tttcgcaatt cggacaaggc gctttttccc tgtcgtaaac
     6421 gcgccagcct ttcgaatagc cgccgtcgta ataactgccg tcgatgtttt ccgggtcaat
     6481 attcaaggtc gagccgtgcg caatcgattc cgccagaatc tcacggattt tttcaaataa
     6541 aacgccggct ttgcgcgccg aaacttcgtt tgccggagtt tgcggattgg cgcgcgccag
     6601 aaacatcgct tccgaagcgt agatgtttcc caagccgcag actttcgtct ggtcgagcag
     6661 aaattccttt aagcttcgct tcgacgtttt caaagccgcg cgaagatatt gcggcgtaaa
     6721 agcgtcggaa aaaggctcgg gcgcgagaca tttcaattct ttcgtttcgt gcaagaaagc
     6781 cgtctcgacg attttcatca agccgaaatg ccgctggtct tgaaaaatca ggcgattttc
     6841 gtcggcgaaa tacaaaactg cgtgcgtgta tttcggcggc tcgcgctcga gcggcaaaat
     6901 cataaagcgc ccggtcattc gcaaatgcgt gataagcgtg ttgccg
//
LOCUS       DASWNE010000048         2286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_355133, whole genome shotgun sequence.
ACCESSION   DASWNE010000048 DASWNE010000000
VERSION     DASWNE010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355133"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1593)
                     /locus_tag="VGB68_01030"
     CDS             complement(<1..1593)
                     /locus_tag="VGB68_01030"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEY0047838.1"
                     /translation="MICSYTAAAKTHVILLKDAPPYTDPTNLEINVGDTVIWKNEGPS
                     LCHVVSDEEARMASEDIMVGKQWQHTFQKAGVYSYLCLRHFFMRGVVTVRNTDGTASS
                     PLEFPYQSAFKEFVVPTEKSVPRMIIPSKVDNTIWFTEGGGDFYGFEGIAPQNKLGKI
                     DESGRIIEFATPTKDGDGSKVAVDSLVMDKQGNVWFTERLAGKVGKLSPDGKIVEFPL
                     SSPTSGPLGIDIDKNGNIWVAQRFANKIAMMTPDGKLTEIELPEPDSEPRTVFVDSRD
                     RVWYTARAANEIGYYDIKTKKLVRLEIPTKTARPAGMWETSDGTIYFVEMVGNKLAKV
                     VGDQIVEFTIPTKFSGAFKIVADAEDNLWFTQVISNSIAKFNTKTHEFIEFKIPTEDS
                     RPGGIAVDRKGRIWFTEQKGNKIAMLDPVKAEALYREQLKKVVPNGEKPAGEKSSVEQ
                     TTPQPANQPKILIKEIKVEDFPLPTLGGAPGNNLLEDDKGWLWFTELHGNKVGALHID
                     SKKFREYPLPTPVSMPTGLARDR"
     gene            complement(1794..2060)
                     /locus_tag="VGB68_01035"
     CDS             complement(1794..2060)
                     /locus_tag="VGB68_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY0047839.1"
                     /translation="MQQANVEEKVIKAIRDLGCDADEILPVHELHLDLGIDSTELVEL
                     TELVRTEFGLQAQRLNLKGIKTVREMVEQMEQVMFETSPSTVNA"
     gene            complement(2093..>2286)
                     /locus_tag="VGB68_01040"
     CDS             complement(2093..>2286)
                     /locus_tag="VGB68_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047840.1"
                     /translation="TLFGSQMPELLFPTRIVGDSLAASGPISIAIGYELLKSRARNYQ
                     SVLALTLGHQGGHIIRMHR"
BASE COUNT          470 a          587 c          580 g          649 t
ORIGIN      
        1 ccggtcccgg gcgagtcccg tcggcatgct aaccggcgtc ggcagcggat attctctgaa
       61 ttttttcgaa tcgatgtgca gagcgccgac tttattgccg tgcagctcgg taaaccagag
      121 ccagccttta tcatcttcca gaaggttatt tcccggcgcg ccgccgagcg tcggcagcgg
      181 aaaatcctcg acctttattt ctttaatcag gattttcggt tgattagccg gttgcggcgt
      241 cgtttgttcg acggatgatt tttcgcccgc aggtttttcg ccgttcggaa cgactttttt
      301 aagctgctcg cggtaaaggg cttcggcttt aaccgggtcg agcatggcga ttttgttgcc
      361 tttttgttcg gtaaaccaga ttcgtccttt gcggtcgacg gcgattccgc ccggtctgga
      421 gtcttcggtc ggaattttaa attcgataaa ctcgtgggtt ttcgtgttga atttggcgat
      481 tgaattggaa ataacctgcg tgaaccacaa attatcttcg gcgtcggcga cgattttgaa
      541 agcgccggaa aatttagtcg gaatcgtaaa ttcgactatc tggtcgccga cgactttcgc
      601 cagtttgttg ccgaccattt cgacaaagta aatcgttccg tcggaggttt cccacattcc
      661 cgccgggcgc gcggttttgg tcggaatttc gaggcgcacg agctttttcg tctttatatc
      721 gtaataaccg atttcattgg cggcgcgcgc cgtataccag acgcggtcgc gggaatcgac
      781 gaaaaccgtg cgcggctcgg aatcgggttc gggcagctcg atttcggtta atttgccgtc
      841 cggcgtcatc atggcaatct tgttggcaaa tctctgggcg acccagatgt ttccgttttt
      901 gtctatatcg attccgagcg gtccgctggt cggagaagaa agcggaaact cgacgatttt
      961 tccgtcgggg ctgagcttgc cgacttttcc ggcaaggcgt tcggtaaacc agacgttgcc
     1021 ctgtttgtcc atcaccagcg aatcgacggc gactttgctg ccgtcgccgt ctttagtcgg
     1081 cgtggcaaac tcgataatgc gtcccgattc gtcgattttt ccgagcttgt tttgcggcgc
     1141 tatgccctcg aacccgtaaa aatctccgcc gccttcggta aaccaaatcg tattgtccac
     1201 cttgctggga attatcattc gcggcacgct tttttcggtc ggcacgacga attctttaaa
     1261 agcgctctgg tatggaaatt cgagcggcga cgaagccgtg ccgtccgtgt tgcggacggt
     1321 tacgacgccg cgcataaaga aatgccggag acacaaataa gagtaaacgc cggctttctg
     1381 aaaggtgtgc tgccattgtt ttccgaccat aatatcttcg ctcgccattc gcgcttcttc
     1441 gtcggaaacg acgtgacaca atgaaggtcc ttcgtttttc cagatgactg tatcgccgac
     1501 gtttatttcc agatttgtcg ggtcggtata aggcggcgca tctttcagca ggattacgtg
     1561 cgtcttggcg gcggcggtat agctgcatat caacaggcaa agccccagca ataccgtccg
     1621 tttaatgttt aaaatcgtca tctaaatcta tcagggcgta aattcgctcc tgcctccttt
     1681 taagctttta aaggcaaatt gtttgtttga cctttcagaa atttgaatga tttgtctgat
     1741 gagcctcgaa taaagactct tcggttgact aaattgagcg cggcgggctc gctctaggca
     1801 tttacagttg aagggcttgt ctcaaacata acctgctcca tctgctccac catttcccga
     1861 accgttttta tccctttcag gttcaggcgc tgcgcctgta atccgaattc ggttctgacc
     1921 agctcggtaa gctcgaccag ttccgtggaa tcgattccca aatcaagatg cagctcgtga
     1981 accggcagaa tctcatcggc gtcacaaccc aaatctctga tcgctttaat cactttttct
     2041 tcgacattag cttgttgcat ataatctcct tttttaaatt ttcttttttg agtcagcggt
     2101 gcatccggat gatgtgtccg ccctgatgac cgagcgtaag cgccaacaca gattgataat
     2161 tccgcgcccg gctctttaac aattcgtaac cgatggcgat tgaaatcggt ccgctggcgg
     2221 ctaagctgtc gccgacgatt cgcgttggaa ataacagttc cggcatttgg ctgccgaaca
     2281 acgttt
//
LOCUS       DASWNE010000049         2908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_37033, whole genome shotgun sequence.
ACCESSION   DASWNE010000049 DASWNE010000000
VERSION     DASWNE010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37033"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..763
                     /locus_tag="VGB68_01045"
     CDS             107..763
                     /locus_tag="VGB68_01045"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEY0047841.1"
                     /translation="MKIYDITVPISENVPIYEGDPKVKIDLTAAIAGGDAANVTQLCF
                     GAHTGTHVDAPNHFIEGTRKIDQLDLEKLIGVARVVEIEENVTAIEPRHVENLETVER
                     ILFKTRNSSFWNEPEAGFRKDFTYITPEAARVLAAKNVKLVGIDYLSVEKFGSDDFAT
                     HITLLEKEIVIIEGLDLRDVPAGEYELICLPLKIISPTGDGAPARTVLRETAKGAHKI
                     "
     gene            783..1328
                     /locus_tag="VGB68_01050"
     CDS             783..1328
                     /locus_tag="VGB68_01050"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF002014.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003950 - NAD+ ADP-ribosyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HEY0047842.1"
                     /translation="MNEKHLVKVSKYLSRHLRHQPERLGLSLETGGWVKVEDLLKACA
                     ENNFPLTLAELKEVVEKDDKRRFSFDASGGKIRANQGHSVEVDLQLKKQTPPARLFHG
                     TAERRAALISERGLSKMARHHVHLSADTETARKVGSRYGKPLIFEIDTMAMLEKGFEF
                     FVSENGVWLVESVPPEFLKVL"
     gene            1325..1606
                     /locus_tag="VGB68_01055"
     CDS             1325..1606
                     /locus_tag="VGB68_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047843.1"
                     /translation="MSESNNVFTVGRKVRIRGECGFAGGVVGMIAYPPKVSVLAEDFG
                     DNYFRKVQTLKGEKAFVWVVFDEPQFDADEDGPYGEAEIDADNLEIIEV"
     gene            1608..1748
                     /locus_tag="VGB68_01060"
     CDS             1608..1748
                     /locus_tag="VGB68_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047844.1"
                     /translation="MNRKEFAERRREMARNSIDELIDLLSSEDLRTRFFAEMCLRDAT
                     ST"
     gene            2066..2779
                     /locus_tag="VGB68_01065"
     CDS             2066..2779
                     /locus_tag="VGB68_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047845.1"
                     /translation="MSEKNWGDTVVGWFIVRDEDETQKAQLETDMTADELIAKYAGSS
                     PLTDETGAETSPAAAEAQKINAVPASYGAPPATVDGQVDFEAVFEAGGVDAEERERIA
                     KAKSLLESLPDATDINTKKQIVEASLKAFGVPIEKIIEAGVAEIEALEFYIRSGAGDT
                     EKVLQDGTTRIQQYELEIQNLRKIMEERVREQNSVVAACNAKKLDVQKVLEFFGRDVV
                     AQVVKDSPKLHEPAETEKS"
BASE COUNT          778 a          672 c          807 g          651 t
ORIGIN      
        1 ccgttttaac tcttcatctc tgttaattct ttttaaaatc cctgcgcttt gcgttcggga
       61 aatatctttt ctccgcgccc gtgcggtaaa ataatcaccg gcaagtatga aaatctacga
      121 catcaccgtt ccgattagcg aaaatgtgcc gatttacgaa ggcgacccga aagttaaaat
      181 cgacctgacg gcggcgattg ccggaggcga tgcggcaaac gtgacgcagc tctgtttcgg
      241 cgcgcacacc ggaacgcacg tcgacgcgcc gaaccatttt atcgagggaa ccagaaagat
      301 tgaccagctt gatttggaaa agcttatcgg cgtcgcccgc gtcgtcgaaa tcgaagaaaa
      361 cgtgacggcg attgaaccgc gacacgtcga aaatctggaa acggtcgaaa gaattttatt
      421 caaaactcgt aactcgtcgt tctggaacga gccggaagcg ggttttcgca aggattttac
      481 atacatcacg cccgaagcgg cgcgcgtttt ggcggcgaaa aacgtcaagc tcgtcggcat
      541 cgactatcta tcggtcgaaa aattcggctc cgacgatttc gcgacgcata ttacgcttct
      601 ggaaaaggaa atcgtgataa tcgaaggctt ggatttgcgc gacgtcccgg cgggcgaata
      661 cgaattgatt tgtctgcccc tgaaaatcat cagcccgacc ggcgacggcg cgcccgcgcg
      721 gactgttttg cgggagacgg cgaaaggcgc gcataaaatc tgaggcgcac attcttcgct
      781 aaatgaacga gaaacacctt gtaaaagtca gcaaatacct gagcaggcac ctgcgtcacc
      841 agcccgaaag actcggtttg agtctggaaa ccggcggttg ggtaaaagtt gaagatttgc
      901 tgaaagcctg cgccgaaaat aattttccgt tgacgctcgc cgagctgaaa gaggtcgtcg
      961 aaaaagacga caaacggcga ttttctttcg acgcgagcgg cgggaaaata cgcgccaatc
     1021 aaggtcattc ggtcgaagtc gatttgcagt tgaaaaaaca aactccgccc gcgcgccttt
     1081 ttcacggaac ggctgagcgc cgcgcggcgc tgattagcga acgcggtttg tcgaagatgg
     1141 cgcggcatca cgttcatctt tcggcggata cggaaacggc gcgaaaagtc gggagccgtt
     1201 acggcaagcc tttgattttt gaaattgata caatggcgat gctggaaaaa ggtttcgagt
     1261 ttttcgtttc cgaaaacggc gtctggctgg tcgaaagcgt tccgccggag tttttaaaag
     1321 ttttatgagc gaatcgaata atgtttttac cgtaggacgg aaagtaagaa ttcgcggcga
     1381 atgcggcttt gccgggggcg ttgtcggaat gattgcttat cctccgaaag tttcggtctt
     1441 agccgaagat ttcggagata actatttcag gaaagttcaa actctgaaag gcgagaaagc
     1501 ttttgtctgg gtggtttttg acgagccgca atttgatgcc gacgaagacg gtccttacgg
     1561 cgaggctgag atagacgcgg ataatcttga gattattgaa gtttgagatg aaccgaaagg
     1621 aatttgccga gcgccgccgc gaaatggcgc gaaattcgat tgacgaattg attgatttgc
     1681 tttcgagcga ggatttacgg acgcgttttt tcgccgaaat gtgcctgcgc gacgcgactt
     1741 cgacctgact gaatttcaag ccggcgagtt cgccggagat gcgccggaaa gccgtcgaag
     1801 aatggcgcgc gtggcgaaaa agcttagccg ggaaatttaa gaaaagattt ggaaatgaga
     1861 aggtgaaaga atgaacgcgg gtgaaaaggt gaaaaagttt tatccgttcc ttttacgctc
     1921 cggcgtcggc aaaaagaaaa cggctttgac cggaaaattt tatttttttt tcttttcggc
     1981 gctttcactt tttcgatttc ccactttaaa acttgccaat aattgcttta ttgcctatat
     2041 ttctaaggcg taaggagtcg aaataatgtc agaaaaaaat tggggcgata cggttgtcgg
     2101 ctggtttatt gtccgcgatg aagacgaaac gcaaaaagcc cagctggaga ccgatatgac
     2161 cgccgacgaa ttaatcgcca aatacgccgg ttcgtcgccg ttgaccgacg aaaccggcgc
     2221 ggaaacttcg cctgccgccg ccgaagctca gaaaatcaat gccgttccgg caagctacgg
     2281 cgcgccgccg gcgaccgtcg acgggcaagt cgatttcgaa gccgtttttg aagcgggcgg
     2341 cgtcgatgcg gaagaacgcg agcgcatcgc caaagccaaa tcgctgctcg aaagcctgcc
     2401 ggacgcgacc gatataaata cgaaaaaaca aatcgtcgag gcttcgctga aagctttcgg
     2461 cgtgccgatt gagaaaatta tcgaagcggg cgtggcggaa atcgaagcgc tcgaatttta
     2521 tatccgcagc ggagcaggcg acacggaaaa agttttgcag gacggcacga cgcgcattca
     2581 acaatacgaa ctcgaaatcc aaaatcttcg taagataatg gaagagcgcg tgcgtgaaca
     2641 gaattcggtg gtcgccgcct gcaacgccaa gaaactggac gtgcagaagg tgctggaatt
     2701 tttcgggcgc gacgtcgtgg cgcaggtcgt caaggattcg ccgaaattac acgagccggc
     2761 ggaaactgaa aaaagttgaa aaaatgtcag ttatcaaaga cgcggagata gggcgacacg
     2821 gagatacgga gagaatttca ccgcgtctcc gtgtctccgg ttctctctgt cacattttaa
     2881 ggagatttaa aaatgtcgat ttttacca
//
LOCUS       DASWNE010000050         6529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_602764, whole genome shotgun sequence.
ACCESSION   DASWNE010000050 DASWNE010000000
VERSION     DASWNE010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6529
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602764"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VGB68_01070"
     CDS             <1..296
                     /locus_tag="VGB68_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047846.1"
                     /translation="GAKARFSARKSIGCFLFLFIFPRAFAGFAALPQLSKIAISTKIP
                     VLSLEIFRLKAKIKNGLEQCENVLNAKSVTAMKCSFVRETNRRRGILCRERSF"
     gene            233..1816
                     /locus_tag="VGB68_01075"
     CDS             233..1816
                     /locus_tag="VGB68_01075"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0047847.1"
                     /translation="MQFCPRDQSQTRHTLPGTQLLAGRYFIERRLGRGAMGQVYLAKD
                     ENLGTRRVAVKTVRQDLLNSDDLQEGEAIVRFEREARSAASISHPHVVDVTDFGKTTD
                     GVFYLVMEYVEGETLHRLLRREGTLTVKRAVNLLKQIAEGVEAAHEAGILHRDLKPAN
                     IFIMQKKSGRSEDGFIKVGDFGLAKIVNQTVTDANSDATPSSRGIIGTPEFMSPEQMQ
                     PELGVDVRADVYALGTIAYLMLGGRTPFAGDLMQLVMQKIMHTPPPLSSLRSDIPADV
                     EQAVMMALEREPAKRPPNVADWISALEKSAEDVSERKSTGKSRVVIMAPVGAEVYVDD
                     ERHGTIGSTGRLILTGIPAGQHVLRVSKAGEKDDDRVIEIREDNAEQVIQAQLKTLQQ
                     GSQPSPSQGSGSVGEQSSILPGIVACSRCSSRYAEGVKFCGRCGNASFTMVSPPEAAQ
                     SYPCPRCSAKLPPFSKFCGRCGLKMGQNQGAQTSAQPISFVSSFPSGIPKPVEKICQR
                     CGNTFPPNIKFCGRCGSNL"
     gene            2139..2690
                     /locus_tag="VGB68_01080"
     CDS             2139..2690
                     /locus_tag="VGB68_01080"
                     /inference="COORDINATES: protein motif:HMM:NF025198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HEY0047848.1"
                     /translation="MKVCPNCNQTYTDESLNFCLNDGATLTNLQTDEPPPTIQMNAAR
                     TTSPNYSDQPTNFTNQPNFGNQPNFGNAPLSNWESQSASPNQPQYMSPPAMATRGQDQ
                     VLPTISLVLGILSIVLFCCYGGIPLGLGALITGYLGMNNANKNPEQYGGRSLAIAGLV
                     LGGLTFVGSILWVLFLIFANALN"
     gene            2756..4060
                     /locus_tag="VGB68_01085"
     CDS             2756..4060
                     /locus_tag="VGB68_01085"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing serine/threonine-protein
                     kinase"
                     /protein_id="HEY0047849.1"
                     /translation="MRVTLRVVSGPHTGRVFTFDQHDTFLIGRTETAHFCLPEDRYFS
                     RHHCLLEMAPPQCFLRDLGSTNGTYVNGMRLETAHLKIGDRIQGGETIIEVEVSSDIV
                     PFSSDPNTYNSTVPSTVTIQCLNCGLLAKTEASRPDAKLSYVCDDCRPKLKNNPQPIP
                     GYQMIRILGQGGMGSVMLATKADDGRAVAIKTLLPEVAVSEQSLKRFMREIEVAASLQ
                     HPHIVSYLEHGTHNGIVYLVTEFVGGMDASKLAKQRGGRLDFREVVKIVEQTLAALDF
                     AHSLGFVHRDIKEQNILVDGNYPNYISKLTDFGLAKSFKQTGMSGVTMVGDVAGTIAY
                     MPPEQIRDFKDVQPPSDIYAMGMTAYTLLTGTHALDLSPRAGIAETVKAIFEKPFVPL
                     VIRNAQLPPRLAEVIEKAISKEPQQRWRTAGEMREAILRAFS"
     gene            complement(4161..5447)
                     /locus_tag="VGB68_01090"
     CDS             complement(4161..5447)
                     /locus_tag="VGB68_01090"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY0047850.1"
                     /translation="MLLRFAKISFLLLVFSLAFMQPYVYIAGFRAVPADIVFLFAFGF
                     WILSLLTKQTRFRWHPFYWLLIFYFAAMLISAVFSVNPRTSSVKLLGEIYLLSLPVLA
                     FNLIENEKDLKRSLKVWLAATGFVVAVGLLTVFLFYFHRENRLLDYTLFSFGTLPPGN
                     YPRVHLTFLNANMLCNYLSVSLMFVLIFQKLGWINKKILYSLLAGILFCAAFTISPSL
                     GGIALSLGVWWWLCFKKSKPFLAKINLAGGIFAGVAFIFAVMIAPSLHPTAPFFIDVP
                     LIEKRVAPSGRVMTWMDSWRTFSENPFVGRGVGQDACDVRYFDLTERLQTLTDAHNVF
                     LNVAAQMGIFGLLAITLIIFYITRKTFPLIFDETNRSVFRIGFGLAFISAFFYQGIGG
                     SYEDARHLWVLIGFFLIQDSGFRIQDSELQILNLES"
     gene            complement(5642..>6529)
                     /locus_tag="VGB68_01095"
     CDS             complement(5642..>6529)
                     /locus_tag="VGB68_01095"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY0047851.1"
                     /translation="SSDNAALFGRYLLEVTTGIPVSLSAPAVHTLYNAKLNLKRAVVI
                     GVSQSGEGTDINTVLERAKNSGAFTVGITNEAESSMAKLADETLLIHAGREKSVAATK
                     TYTGQMLHFYMLASALAREKIAFEKIPDFAAQALELRPEVEKLVERYVFMENCVVVGR
                     GMNYGNAYELALKLMETCYVVAERFSSADFFHGPLAIVERRFPVILFAPQGVAQRGNV
                     DLLNRLQELHADCFTITNDETVARLSSRSLKMPAEIDEFLSPIPFIIPAQLFAGLLAA
                     AKGIDADAPRSLSKVTRTI"
BASE COUNT         1665 a         1691 c         1732 g         1441 t
ORIGIN      
        1 gaggagcaaa agcgaggttt tccgcgagga aatcgatcgg ctgcttttta tttttattta
       61 tctttcctcg cgcctttgca ggctttgcgg ctttgcctca attatcaaaa attgcaattt
      121 caacgaaaat acccgttctt agtttggaaa ttttccggct taaagcgaaa ataaaaaacg
      181 ggttggaaca atgcgagaat gtcctaaatg cgaaatctgt tacggcgatg aagtgcagtt
      241 ttgtccgcga gaccaatcgc agacgcggca tactctgccg ggaacgcagc ttttagccgg
      301 acgatatttt atcgagcggc gattggggcg cggcgcaatg ggacaggttt atctcgccaa
      361 agacgaaaat ttaggaacgc gccgcgtcgc cgtcaaaacc gtccgccagg atttattgaa
      421 ttccgacgat ttgcaggaag gcgaggcgat cgtgcgtttc gagcgcgagg cgcgctcggc
      481 ggcttcgatt agtcacccgc acgtcgtcga cgtgacggat ttcggcaaaa cgacggacgg
      541 cgttttttat ctggtgatgg aatacgtcga gggcgagacg ctgcaccggc tcctgcgccg
      601 cgagggaact ttaaccgtca agcgcgccgt caatctgctc aaacaaattg ccgaaggcgt
      661 cgaagccgcg cacgaagcgg gcattctgca ccgcgattta aaacccgcca acatctttat
      721 aatgcagaaa aaatccggca ggtccgaaga cggttttatc aaggtcggcg atttcggact
      781 ggcgaaaatc gtcaatcaaa ccgtcacgga cgccaactcc gacgcgacgc cttcttcgcg
      841 cggaattatc ggcacgccgg aatttatgtc gcccgagcag atgcagccgg aactcggcgt
      901 cgacgtgcgc gccgacgttt acgcgctcgg cacgattgcc tatttaatgc tcggcggcag
      961 aacgccgttt gcgggcgatt tgatgcagct ggtgatgcag aaaattatgc acacgccgcc
     1021 gccgctttct tctctgcgct cggatattcc ggcggatgtc gagcaggcgg taatgatggc
     1081 attggaacgc gagccggcga agcgtccgcc gaatgttgcc gattggattt cagcgctgga
     1141 aaaatcggcg gaagatgttt ccgaaaggaa aagcaccgga aaatcgagag ttgttattat
     1201 ggctccggtc ggcgcggagg tttatgtcga cgacgagcgg cacggcacaa tcggcagcac
     1261 aggcagattg attctgaccg gaattccggc gggacaacac gttttgcgcg tatcgaaagc
     1321 cggcgaaaag gacgacgacc gcgtgattga aatccgcgaa gacaacgccg agcaggtgat
     1381 tcaggcgcag ctgaaaacat tgcagcaggg aagccagccg tcgccgtcgc agggcagcgg
     1441 cagcgtcggc gagcagtcga gcattttgcc cggcatcgtc gcctgcagca gatgcagctc
     1501 gcgctatgcc gaaggcgtaa aattctgcgg tcgctgcggc aatgcgagtt ttacaatggt
     1561 cagtccgccc gaagcggcgc agtcgtaccc gtgcccgcgc tgttcggcaa agcttccgcc
     1621 gttttcaaaa ttctgcggtc gctgcggttt aaaaatggga caaaatcagg gcgctcagac
     1681 ttcagcccag ccgattagct ttgtgtcgag ctttccgtcc ggcattccaa agccggtcga
     1741 gaaaatctgc cagcgctgcg gcaacacttt cccgccgaat ataaaatttt gcggtcgctg
     1801 cggctcgaat ctttaacggc gacaatgaac gccggacgcg aaatgacgaa ctgcgggctc
     1861 tcgtattcca aattcatcgt ttcgcgtccg gcgttcgtcg tttctccgct cctgctggta
     1921 acttcaatca aacttagcgt ataatgccac agtcggataa aagcccccga ggaaatttcg
     1981 ttgatgaaaa tccgtccgca tcgtaatgaa acctacgccg acgattcaat gaatttctgt
     2041 ttgtctgacg gttcgatttt aacgccgccg gcgatgccgt gccggctgct ttttgcggct
     2101 tggaaattag aaaataagag aaaagcttaa ttcaaattat gaaagtttgc cctaattgca
     2161 atcagactta cacggacgaa tcgctgaatt tttgtttaaa tgacggcgcg accttaacga
     2221 atctgcaaac ggacgagccg ccgccgacca tccagatgaa tgcggcgcgc acgaccagtc
     2281 cgaattattc cgaccagccg acgaatttta ccaaccagcc taacttcggc aatcagccta
     2341 atttcggcaa cgcgccgctt tccaactggg aaagccaatc ggcgtcgccg aatcagccgc
     2401 agtatatgtc gccgccggcg atggcgacgc gcgggcaaga ccaggtgctg ccgaccattt
     2461 cgctggttct gggcattctc agtatcgtat tattttgttg ttacggcggc atcccgctcg
     2521 ggctcggcgc gctgattacg ggttatctgg ggatgaacaa tgcgaataaa aatcccgagc
     2581 aatacggcgg acgcagtctg gcgattgccg gattagtatt gggcggactg acctttgtcg
     2641 gctcgattct gtgggttttg tttcttatct tcgccaatgc gcttaattaa taagagaagt
     2701 ttttgaagct tccgttttgt atatttgaag cttttattcg gcaaaagatt ttacaatgcg
     2761 agtaactttg cgagtggttt cgggtccgca tacgggacgc gttttcacgt ttgaccaaca
     2821 cgacaccttt ttaatcggac gcacggaaac ggcgcatttc tgtctgcccg aagaccgtta
     2881 tttttcccgt catcactgtc tgttggaaat ggctccgccg caatgctttc tgcgcgattt
     2941 gggctcgacc aacggaactt acgtcaacgg gatgcgtctg gaaaccgctc acttaaaaat
     3001 cggcgaccgc atacagggcg gcgaaacgat tatcgaagtc gaggtatcgt cggacatcgt
     3061 ccctttttct tccgacccga acacatataa ttctactgtt ccgtcgaccg tcacgattca
     3121 atgtttaaac tgcggtctgc tggcgaaaac cgaagcctcg cgccccgatg ccaagctttc
     3181 ctacgtctgc gacgactgcc gcccgaaact gaaaaataat ccgcagccga ttcccggcta
     3241 ccagatgatt cgcatcctcg gtcagggcgg catgggcagc gtgatgctgg cgacaaaggc
     3301 ggacgacggg cgcgccgtcg ccatcaagac gcttttgccg gaagtcgccg tcagcgaaca
     3361 gtcgctgaaa cgctttatgc gcgagattga ggtcgccgcc agccttcagc acccgcatat
     3421 cgtcagctat ctcgaacacg gaacgcacaa cggtatagtt tatctcgtca ccgaattcgt
     3481 cggcggaatg gacgcttcaa aactggcgaa acagcgcggc ggaaggctcg attttcgaga
     3541 ggtcgtgaaa attgtcgaac agactctggc ggcgctggat ttcgcgcatt cgctcggctt
     3601 cgttcatcga gatattaaag agcagaacat tctggttgac ggaaattacc cgaattacat
     3661 atcgaagctg acggatttcg gcttggcgaa aagcttcaaa caaaccggaa tgagcggcgt
     3721 cacgatggtc ggcgacgtag cgggaaccat cgcctatatg ccgcccgaac aaatccgtga
     3781 ttttaaggac gtccagccgc cgtcggatat ttatgcgatg ggaatgacgg cttacacttt
     3841 gctgaccggc acgcacgcgc tcgatttaag cccgcgcgcc ggaatcgccg aaacggtcaa
     3901 agcgattttt gaaaaaccgt tcgtgccgct ggtaatcaga aacgcgcagc ttccgccgcg
     3961 tctcgccgaa gtcatagaaa aagcgatttc caaagagccg cagcagcgct ggcgcacggc
     4021 gggcgagatg cgcgaagcta ttctgcgagc gttcagctaa aggcaggatt caagatttag
     4081 gattcaggat tcaggattta ggattcaaga tttaagattc aggattcaga atttaggatt
     4141 caggattcag gattcaggat ttaagattca agatttaaga tttgtaattc tgaatcctga
     4201 atcctgaatc ctgaatcctg aatcagaaaa aatccaatca aaacccacag gtggcgcgcg
     4261 tcttcatacg agccgccgat gccctgataa aaaaacgcgc tgataaaagc cagcccgaag
     4321 ccgattcgaa aaacgctccg gtttgtctcg tcaaaaatca atggaaacgt ttttcgggtg
     4381 atataaaaaa ttatcaaagt tatcgccagc agcccgaaaa ttcccatctg cgcggcgacg
     4441 ttcagaaaaa cgttgtgcgc gtcggtcaat gtctgcagtc tttccgttaa atcaaaatac
     4501 ctgacgtcgc aggcgtcctg cccgacgccg cgcccgacga aagggttttc cgaaaaagtt
     4561 ctccacgaat ccatccaggt cattacccgc ccggacggcg cgacgcgttt ttcaatcaat
     4621 ggaacgtcga taaaaaacgg cgcggtcggg tgcaggctcg gcgcaatcat caccgcgaaa
     4681 ataaacgcga cgcccgcaaa aatgccgccc gccagattta ttttggcaag gaaaggcttc
     4741 gattttttga aacaaagcca ccaccaaacg cccaggctca aagcaatccc gccgaggctc
     4801 ggcgaaatcg taaaagccgc gcaaaataaa ataccagcca ggagcgagta aagaattttt
     4861 ttatttatcc agccgagctt ttgaaaaatc aaaacgaaca tcaggctgac gctcaaataa
     4921 ttacagagca tattggcgtt taaaaacgtc aagtgaacgc gcggataatt gccgggcgga
     4981 agcgtcccga aactgaaaag cgtgtaatcg agaagccggt tttcgcggtg aaaataaaaa
     5041 agaaaaaccg tcagcagtcc gacggcgacg acaaagcccg tcgccgccag ccacactttc
     5101 agcgagcgct tcaaatcctt ctcgttttca atcagattga aagccagaac cggcagactg
     5161 agcagataaa tctcgcccag aagctttaca gagctcgttc gcggattaac cgaaaaaacg
     5221 gctgaaatca gcatcgccgc gaaataaaaa atcagcagcc agtaaaacgg atgccagcga
     5281 aaacgcgtct gttttgttaa taacgataaa atccagaagc cgaaagcgaa aagaaaaaca
     5341 atgtccgccg gaacggcgcg aaatcccgcg atataaacgt aaggctgcat aaaggcgagc
     5401 gagaaaacga gcagcagaaa cgagattttg gcgaaccgca aaagcatatt ctctaatgca
     5461 aaaaaccagg taagattttt accacggaga cacggaggca aagagaaaaa ttaacaggga
     5521 tggaggagat agacagggat ttttaaaatt tatttcttat cccctttatc tcctccatcc
     5581 ctgttaattt ttctttaaaa aacctctgtg cctccgtgtc tccgtggtaa atcatttttt
     5641 cttaaatcgt gcgcgtcact ttggaaagcg agcgcggcgc gtccgcgtct atgcctttgg
     5701 cggcggcgag cagcccggcg aaaagctgcg cgggaatgat aaacggaatc ggcgacagaa
     5761 attcgtcgat ttccgcgggc attttcagcg agcgcgagct gagccgggcg accgtttcgt
     5821 cgttggtaat cgtaaaacaa tcggcgtgga gctcttgcag gcggttcagc aaatccacgt
     5881 ttccgcgttg cgcgacgccc tgcggcgcga acagaatcac cggaaagcgg cgctcgacga
     5941 tggcgagcgg accgtgaaaa aaatcggcgg acgagaatct ttcggcgacg acgtagcagg
     6001 tttccatcaa ttttaaggca agctcgtaag cgtttccgta attcattccg cgcccgacga
     6061 cgacgcagtt ttccataaaa acgtagcgct cgacaagttt ttcgacttcc gggcgcagct
     6121 ctaaagcctg cgccgcaaaa tccggaattt tttcaaaagc gattttttcc cgcgccagag
     6181 ccgaagcgag catataaaaa tgcagcatct gcccggtgta agtcttcgtg gcggcgacgc
     6241 tcttttcgcg tccggcgtga attaaaagcg tttcgtcggc gagctttgcc atcgaagatt
     6301 cggcttcgtt ggtgattccg accgtgaacg cgccggaatt tttggctcgt tccaggacgg
     6361 tgttgatgtc cgtgccttcg cccgattgcg aaacgccgat gacgacggcg cgttttaaat
     6421 ttaatttcgc gttatagagc gtgtgaacag ccggagccga cagcgagacg ggaattccgg
     6481 tcgtcacttc cagcagatat cgaccgaaaa gcgcggcgtt atcggagct
//
LOCUS       DASWNE010000051         4118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_319136, whole genome shotgun sequence.
ACCESSION   DASWNE010000051 DASWNE010000000
VERSION     DASWNE010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319136"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VGB68_01100"
     CDS             complement(<1..195)
                     /locus_tag="VGB68_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047852.1"
                     /translation="MRKSFTLFCNLLLIISITATSFAQTATSSRAITGATKEKVVAPS
                     KSNPVTVEKIEQDMAEALTVI"
     gene            339..698
                     /locus_tag="VGB68_01105"
     CDS             339..698
                     /locus_tag="VGB68_01105"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY0047853.1"
                     /translation="MFRHISILFLLCLFGFCVAVKAQTETYKPLKILSKPAANYTNAA
                     RANNIQGRVRVKVTFMANGKIGKVSDEPQNHEDVKKSGLVKAAIEAAKKIRFEPAIKN
                     GKPVTAVKIIEYTFTLY"
     gene            755..1780
                     /locus_tag="VGB68_01110"
     CDS             755..1780
                     /locus_tag="VGB68_01110"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0047854.1"
                     /translation="MSHLLEVQNLQTHFPTRAGLVKAVNDVSFYIDEGELLGLVGESG
                     CGKSITALSVMRLIAPPGKIVGGTIKFKGEELTTASVERLRQIRGNDIAMIFQDPMTS
                     LNPVYTVGEQIAEALRLHRNLNKKQAWEAAIASMKEVSIPDPARRVNDYPHQLSGGMR
                     QRVMIAMALACDPELLIADEPTTALDVTIQAQILELLDNLRKTRKLAVLLITHDLGVV
                     AEVADRVAVMYTGKIVEESGVEEIFRQPKHPYTQGLLRSVPKLREFGDEKITRLSTIQ
                     GTVPSPANLPEGCHFAPRCEYRMDICTKGEIPLYRINEEVKARCVLYDENVKTAQREK
                     SVSAVSE"
     gene            1758..2267
                     /locus_tag="VGB68_01115"
     CDS             1758..2267
                     /locus_tag="VGB68_01115"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY0047855.1"
                     /translation="MSLRFPNDDDFAEFTALNKSSRKFHRNLVTPPTDEKSFADFLKR
                     NESETDRCFLICRIEDGAIVGAAALSQIFRKAFQNAYLGYYLAEKYTGCGYMTEAVRL
                     VLSFAFKELKLHRIEASVQPENKASIAVLQRCGFTREGYSPKYLKIGGRWRDHERFAI
                     IVEDWRKGK"
     gene            2264..2929
                     /locus_tag="VGB68_01120"
     CDS             2264..2929
                     /locus_tag="VGB68_01120"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEY0047856.1"
                     /translation="MSQVLKEKKLMTVEQYLRFEERSKYKHEYMDGEIFRLHDNNESP
                     QLAGNRARHVFIVSNISFYLRLALREKNKNCQVGTTEMRVLLRENHYAYPDVFAVCDE
                     LSLVPDIFDTLENPIVIFEVLSKSTEIRDRGDKAVDYRKLKTLTDYILVSQDKMRVEQ
                     QTRQTDGTWKLLVFESADDKIYIASLKCEIVIADIYENISFPVKPSLKLVNSKKKNGN
                     KRS"
     gene            2937..3926
                     /locus_tag="VGB68_01125"
     CDS             2937..3926
                     /locus_tag="VGB68_01125"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEY0047857.1"
                     /translation="MEAKELIRVRNLVKHFAVENSDDVVRAVDDVSFDIFAGETLGLV
                     GESGCGKSTVGRCLLRLIEPTGGEVWFENRNVLNLGKQDLRELRREMQIIFQDPYASL
                     NPRLTILSIIGEPLKIHGIGSKSEQKERVADLLKKVGLDPNYMHRYPHEFSGGQRQRL
                     GIARALALNPKLIIADEPVSALDVSVQAQVVNLLQDLQQELGLTYLFISHGLAIVEHI
                     SQRVAVMYLGKIVEIADSRELYENPVHPYTKALLSAIPIPDPTRKRERIVLQGDVPTP
                     INPPSGCRFRTRCPWAIDDCAQVVPELREIKVGHFAACIRVEGYANAPSLEKS"
BASE COUNT         1106 a          971 c         1061 g          980 t
ORIGIN      
        1 aatgacggtc aaggcttcag ccatatcctg ttcgattttt tcgaccgtta ccggatttga
       61 tttgcttggc gcgacgactt tttccttcgt cgcgcccgta atcgctctgg agctggtcgc
      121 ggtttgcgca aaagatgtcg cggttatcga aataattaat aaaagattgc agaaaagggt
      181 aaaacttttt ctcacggaca aaatctcctt aataaaggct tgctgttaag cacaagaaat
      241 gcgaaaaaat gataacattt ctttgaagta aaagcgatga aaagagttgc acgcgataga
      301 caggcggcgg aaacttttct gcgaaaggaa aaataaagat gttcaggcat atttcgattc
      361 tgtttctact ttgtttattc ggcttttgcg tggcggtcaa agcgcagacg gaaacctaca
      421 aaccgttgaa aattctgtca aaaccggcag ccaattacac gaacgccgcc agagcgaata
      481 atattcaggg cagggttcgg gtcaaagtca cgtttatggc aaacggaaaa atcggcaagg
      541 tgagcgatga gccgcagaat cacgaagatg taaaaaaatc cggtctggtt aaagccgcta
      601 tagaagcggc gaaaaaaatc aggtttgaac cggcgataaa gaatggcaag ccggtgacgg
      661 cggtaaaaat tatagaatat acctttacgc tttattaaat ttattaaatt ttctgcggcg
      721 aaattgtttg aatccgtttt tacgttatat tgcaatgtct catttattag aagtccaaaa
      781 tttacaaact catttcccga cgcgcgccgg tctggtcaaa gccgtcaacg acgtcagttt
      841 ctacatagac gaaggcgagt tgctcgggct ggtcggcgag tccggctgcg gaaaatcaat
      901 cacggcgctg tcggtaatgc gcctgattgc gccgccggga aaaatcgtcg gcggcacgat
      961 aaaatttaaa ggcgaagagc tgacgaccgc ttcggtcgaa cgcctgcgcc agattcgcgg
     1021 caacgacatc gcgatgattt ttcaagaccc gatgacctcg ctcaatcccg tctacacggt
     1081 cggcgagcag attgccgaag ctttgcggct gcatcgcaat ttgaacaaaa aacaggcttg
     1141 ggaagcggca atcgcgtcga tgaaagaagt ctcgattcca gacccggcgc ggcgcgtcaa
     1201 cgattatccg caccagcttt ccggcggaat gcgccagcgc gtgatgattg cgatggctct
     1261 ggcttgcgac cccgaattat taatcgccga cgagccgacg accgcgctcg acgtcaccat
     1321 ccaagcccag attctcgagc ttctggataa tctcagaaaa accagaaagc tcgccgtttt
     1381 gcttatcact cacgatttgg gcgtcgtcgc cgaagttgcc gatagggtcg ccgtaatgta
     1441 cacgggaaaa atcgtcgagg aatcgggcgt cgaggaaatc tttcgccagc cgaaacatcc
     1501 ttacacgcag ggacttttgc gctcggttcc gaaattgcgg gaattcggcg acgaaaaaat
     1561 cacgcggctt tcgacgattc agggcacggt gccgagcccg gcgaatctgc ccgaaggctg
     1621 tcattttgcg ccgcgctgcg aataccggat ggacatctgc acgaaaggcg agattccgct
     1681 ttatcgaatt aacgaagaag taaaagcccg atgcgtctta tacgatgaaa acgtcaaaac
     1741 tgcccaaagg gaaaaaagtg tctctgcggt ttccgaatga cgatgatttc gctgaattta
     1801 cggcgctgaa taaatcgagc cgtaaatttc accgcaatct ggtcacgccg ccgaccgacg
     1861 aaaaaagctt tgccgatttt ttaaagcgca atgaatcgga aaccgacagg tgttttctca
     1921 tctgtcgcat cgaagacggc gcgattgtcg gcgcagcggc gctttcgcag atttttcgca
     1981 aagcttttca aaacgcttat ctcggttatt atttagccga aaaatatacg ggctgcggct
     2041 atatgaccga agccgtccga ttggttttga gtttcgcatt taaagaatta aagctgcacc
     2101 gcatcgaagc cagcgtccag ccggaaaaca aggcttcaat cgccgttttg caaagatgcg
     2161 gctttaccag ggaaggttac tcaccgaaat atctgaaaat cggcgggcgc tggcgcgacc
     2221 acgagcgctt tgcgattatt gtcgaagatt ggagaaaagg aaaatgagtc aggtcttaaa
     2281 agagaaaaaa ttaatgaccg tcgagcagta tctgcgtttt gaagaacgct cgaaatataa
     2341 gcacgaatat atggacggcg aaatttttcg cctgcacgat aataatgaat cgccgcaatt
     2401 agcgggaaat cgcgcgcggc acgtttttat cgtcagcaat attagtttct atttgcggct
     2461 cgctttacgt gaaaaaaaca aaaactgtca ggtcggaacg acggagatgc gggttttgct
     2521 ccgcgaaaat cattacgcgt atcctgatgt ctttgcggtt tgtgacgaat tatcgcttgt
     2581 gccggatatt tttgacactt tggaaaatcc aatcgtcatt tttgaagttt tatcaaaatc
     2641 caccgaaatt cgcgaccgcg gcgacaaggc tgtggattat cgaaaattaa aaaccttaac
     2701 cgattatatt ctggtatctc aagacaaaat gcgcgtcgag cagcaaacac gccaaaccga
     2761 cggaacctgg aaacttctgg tttttgaaag tgccgacgat aaaatttaca tcgcttcgct
     2821 caaatgtgaa attgtcatcg cagatattta tgaaaacatt agttttcctg ttaaaccaag
     2881 cctgaaactc gtcaactcga aaaagaaaaa tggaaacaag cgcagttaaa ttaaaattgg
     2941 aagcaaaaga attaatccgg gtcagaaatc tggtcaaaca tttcgccgtc gaaaacagcg
     3001 acgacgtggt gcgcgcggtc gacgacgttt cattcgatat tttcgcgggc gagacgctcg
     3061 ggctggtcgg cgaatccggc tgcggaaaat cgaccgtcgg gcgctgtctt ttgcggctca
     3121 tcgagccgac cggcggcgaa gtctggtttg aaaatagaaa cgttctgaat ctcggcaaac
     3181 aggatttgcg cgagctgcgg cgcgagatgc agattatttt tcaagaccct tatgcttccc
     3241 tgaatccgcg cctgaccatt ctttccatca tcggcgagcc gctgaaaatt cacggcatcg
     3301 gcagcaaatc cgagcagaaa gagcgcgtcg cggatttgct gaaaaaagtc gggctagacc
     3361 cgaattatat gcatcgttac ccgcacgaat tttccggcgg tcagcgccag cggctgggaa
     3421 ttgcgcgcgc gctggctttg aatccgaagc tgattatcgc cgacgagccg gtttcggcgc
     3481 tggacgtttc ggtgcaggcg caggtcgtca atcttttgca ggatttgcag caggaattgg
     3541 gtttgactta tctttttatc tcgcacgggc tggctatcgt cgagcatatc tcgcagcgag
     3601 tggcggtgat gtatctcgga aaaatcgtgg aaatcgccga ttcgcgcgag ctttacgaaa
     3661 acccggtgca tccttacacc aaagcgctgc tctcggcgat tccgattccc gacccgacgc
     3721 gcaagcgcga gcgcatcgtt ttgcagggcg acgtgccgac gccgataaat ccgccttcgg
     3781 gctgtcgttt cagaacgcgc tgcccgtggg cgattgacga ttgcgcgcaa gtcgtgccgg
     3841 agctgcgcga gattaaggtc ggtcatttcg ccgcctgtat ccgcgtcgaa ggctacgcaa
     3901 atgcgccgag cctggagaaa tcataattga aacgttccgg ttaaatttta cgtaaaagca
     3961 ggagaaccgt taacccgaca gatgcaaaga atcgaaaacg cagagtaagt atgaacccga
     4021 aacgaaattt aacgttttgc ttgccgccgg ggtcgtcgcc cgcgcgtctt tcaggttgaa
     4081 ttttttcgca gattaatttg aatctttttt gatgaagg
//
LOCUS       DASWNE010000052         5302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_249327, whole genome shotgun sequence.
ACCESSION   DASWNE010000052 DASWNE010000000
VERSION     DASWNE010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5302
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249327"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="VGB68_01130"
     CDS             <1..916
                     /locus_tag="VGB68_01130"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY0047858.1"
                     /translation="FRKVTPYMTNLKNPNSAAFYTDADGQDWFYLAETDKITRRKFTR
                     GEIKPTAKAETVATFPDYGLSYKYGGWHLTRSLAFAPSGKLYVSVGSSCNACDEKPTE
                     KERATVQEMNPDGSEKRTFAKGLRNAVGLKWIGNSLWATNQGSDHLGLGKPDETFYSL
                     KDGSDYGWASCYQSNGKIFFDTTPPNRLKKPTARLKSCADVPVSYAYFPARSSPMGFD
                     YFDAPGTDEIIKNAFLVSLHGSTNKKIGHGYKIVIMRKGEKLQDFINGFLQGQTVYGR
                     PCDIMRLDDDSFLFTDDFSGVVYYVRKK"
     gene            926..1546
                     /locus_tag="VGB68_01135"
     CDS             926..1546
                     /locus_tag="VGB68_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047859.1"
                     /translation="MKNKNNNQNSLIGLPEGDEVREAFYRLSGRVEELGVNIDENFSA
                     PDHARFIGIFEAHTIILYPRLAPPFALWFTVAHLFGHMTQLVNETPRVERANELVLST
                     GKSLTPEDVQLIYDHEREAAEIGRALIADVDAALSPELDTAYTRFFHADFRYLINFIE
                     TGAAGVQVFERYWRHEPNPRELIAADARKLLDMKDFPASDERIVVV"
     gene            2285..2464
                     /locus_tag="VGB68_01140"
     CDS             2285..2464
                     /locus_tag="VGB68_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047860.1"
                     /translation="MDDKYQISKSKEPETKQEIIQHKIEELVKKRDKETSPSAKKALN
                     AEIMKLFAQYERLKL"
     gene            2950..3186
                     /locus_tag="VGB68_01145"
     CDS             2950..3186
                     /locus_tag="VGB68_01145"
                     /inference="COORDINATES: protein motif:HMM:NF020006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HEY0047861.1"
                     /translation="MIIAGVIKTMIYELLEADTAKDLELKINQKLAAGWQLYGDLVTA
                     IKPDTSEVRGGIGFAVESKVILFQAIIKNERAKE"
     gene            3423..4565
                     /gene="bshA"
                     /locus_tag="VGB68_01150"
     CDS             3423..4565
                     /gene="bshA"
                     /locus_tag="VGB68_01150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HEY0047862.1"
                     /translation="MNIGITVYPTYGGSGIVGSELGRELALRGHNVHFISSMLPTRLT
                     ELNERVHFHEVEMMSYPLFEHQPYDLALATKMATVARSEKLDLLHVHYAIPHSISAIL
                     ARESIKQKRYVPVITTLHGTDITLVGADRSYLPITRYGLQQSDGVTAVSKFLQKATIE
                     TFDFDEIEVIPNFICGCHYKHLPESPLRDELAPNGEKLLVHVSNFRAVKRPVDCIEIL
                     AKVKEKGGKARLIMVGDGPERSACYYRAEQLGVKDDTVFVGKQANIPDYLGIADVFLL
                     PSELESFGLAALEAEACEVPVIATRIGGIPEVVSDGETGFLSDIGDTEKMSDDVMKFL
                     NDEEMRHSFGEKARESAVSRYGSQLIIPQYINFYERILGKTKSAAV"
     gene            4694..>5302
                     /locus_tag="VGB68_01155"
     CDS             4694..>5302
                     /locus_tag="VGB68_01155"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047863.1"
                     /translation="MKYLDWIIVIAYLVYVIWDGIRLTKHSNTSDGYFLASRSLPWWA
                     VGLSVMATQLSAITLVGTTGQAYSDGMRFIQFYYGLPLAMIILCVTVVPFFHRAKVYT
                     AYEYLEKRFDVKTRALTSFFFLISRGLGVGTIISAPSIVLSIVFGWNLIFTIFAIGMS
                     TTVYTVFGGVQAVTWTDVKQMFVIFFGLAVCLIVILSSFPTGV"
BASE COUNT         1414 a         1235 c         1357 g         1296 t
ORIGIN      
        1 gtttcgcaaa gtcacgccgt atatgaccaa cctgaaaaat ccgaacagcg ccgcctttta
       61 taccgacgcg gacgggcagg actggtttta tctggcggaa accgacaaga ttacgcgccg
      121 gaaattcacg cgcggcgaaa tcaagccgac cgcaaaagcg gaaaccgtgg cgacttttcc
      181 cgattacggc ttgagctaca aatacggcgg ctggcacctg acgcgcagcc tcgcttttgc
      241 gccgagcggc aagctttacg tatcggtcgg cagcagctgc aacgcctgcg acgaaaagcc
      301 cacggaaaaa gagcgcgcga ccgttcagga aatgaacccg gacggcagcg aaaagcgaac
      361 ttttgcgaaa ggcttgcgca acgccgtcgg tttgaaatgg atcgggaatt cgctctgggc
      421 gaccaatcaa ggctcggacc atctgggatt gggcaaaccg gacgaaactt tttacagttt
      481 gaaagacggc tccgattacg gctgggcttc gtgctaccag tcaaacggca aaatcttttt
      541 tgatacgacc ccgccgaatc gccttaaaaa gccgaccgcg cgtctgaaat cgtgcgccga
      601 tgtgccagtc tcttacgctt attttcccgc ccgttcgtcg ccgatgggct tcgattattt
      661 cgacgcgccc gggacggacg aaatcatcaa aaacgcattt ttagtttcgc tgcacggctc
      721 gaccaacaaa aaaatcgggc acggctataa aatcgtcatt atgcggaaag gcgaaaagct
      781 tcaggatttt ataaacggtt ttctgcaggg acaaaccgtc tacggtcgcc cgtgcgacat
      841 tatgcggctc gacgacgatt cgtttctttt tacggacgat ttcagcggcg tcgtttatta
      901 tgtgagaaag aaatgaaaaa caaaaatgaa aaacaaaaat aacaatcaaa acagtctgat
      961 aggtctgccg gaaggcgacg aagtaagaga ggcgttttac cggcttagcg ggcgcgtcga
     1021 agagctcggc gttaatatcg acgaaaattt ttccgcgccc gaccacgcca gatttatcgg
     1081 aatcttcgaa gctcacacga ttattctgta tcctcgcctc gcgccgccgt tcgctctctg
     1141 gtttacggtc gctcacctgt tcgggcatat gacgcagctg gtgaacgaaa ctccgcgcgt
     1201 cgagcgcgcc aacgagcttg tcctgagcac gggaaagagc ctgacgcccg aggatgtgca
     1261 gttgatttac gaccacgagc gcgaggcggc ggagatcggc agggcgctga tagcggacgt
     1321 agacgcggcg ctttcgcccg aactggacac ggcttacacg cgtttctttc acgcggattt
     1381 tcgttatttg attaacttta tcgaaaccgg cgcggcgggc gttcaggtgt ttgagcgtta
     1441 ttggagacac gagccgaatc cgagggagct gatagcggcg gacgcgcgga agcttctgga
     1501 tatgaaggat tttccggctt cggacgagcg aatcgtggtc gtttgaaaat tttcgtgtaa
     1561 ttttcagcgc cgccgctgac cgcaggaacg gcaaaaatat tgcgaggttg taataaaaac
     1621 cagacacagc gcgctggtgg caaataaaat aatcgccatc ggtgtaaagg attcgagctc
     1681 gagcaagcct attaatccag cgccggagat ggttagagaa atgattacag ccgtcggggt
     1741 aaatctttta tttacccggt agcagcctgt gttagagccg caaaaccgac atttctttct
     1801 tttcatacca atatagacag taaatgggtc ggattgattt atacaatatc tgcgtatagt
     1861 aaacaagcaa atttgaaaat ctcgcgggtt ttatttacta aaacaaggct ttcccttttc
     1921 tttttcgctg attgttcttt cgatacaacc cgaaacctaa gacggctaag aaaagtcccg
     1981 ccgttaaaaa gacgccggat gataaagcgt cttcatcgat tctgaagatg aaagcagcaa
     2041 tcaggaaaaa gaaggataaa ataagggata ggtaagcggt cgcgcgaaga ttgctcatat
     2101 ctgtttctcc attattacta tttcagtaaa gaaccaatct tcagtaaaga actaatcgag
     2161 cgatatttta ccgttttttt cagaggtctg atgatagccg ggcgcgcgcg gattcggatg
     2221 cttcaaagct ccgggtccgg caaataaccg ggtgggcgga catatcttgt agtaaaatcg
     2281 atttatggac gataaatatc aaataagcaa aagcaaagag ccggaaacaa aacaggaaat
     2341 tatccagcac aaaatcgaag aactggttaa aaagcgggac aaggaaacca gcccttccgc
     2401 taaaaaggct ctcaatgcgg aaattatgaa gctgtttgcg caatacgaaa gactgaaatt
     2461 ataagatgaa tttttatcgc gacagccttt ttaattggac ttttaccggt tacgacttct
     2521 tcgagataaa gtagattccg atgattgaat atacgattta ttgcgacgtt tgcggagaaa
     2581 tgattgacat tcaatcggga agcgctcgcc aagccagaaa aaaagcaaaa gccaaaggat
     2641 tactcgttcg cattcttcgc aaagactatt gcccgccttg cgccgaaaaa cttcaaaatg
     2701 aaggagaaat cgataattcc gtaaccggga attaaacgcg gaaaagcggc ataagggaat
     2761 aactaaccgg tttcgagacg ccttttaaaa ttttatgaac gctctgcgcc ggcataagaa
     2821 taatgtcagg ctgataactg cgtttgcgca ggattttttt tgtgataaat tgacgagtga
     2881 agaaagatta tccaacaacg aaagaaacgg cgctgcgtcc gggcggtttc agccgcgccg
     2941 tttgggtaaa tgattattgc aggcgtgata aaaactatga tttatgaact tttagaagcg
     3001 gatacagcca aggatttgga acttaaaata aatcaaaaac tggctgccgg ctggcagctc
     3061 tacggagatt tggtaacggc aatcaagccc gacacttccg aagtgcgtgg cggcatcggc
     3121 tttgccgtgg aaagtaaagt aatactgttt caggcaatta taaaaaatga acgggctaaa
     3181 gaataatttc gagaaaaaat aaagccttcg gttcgtaatc agccgacagg cttcgatatt
     3241 tgataattga ataagctctg acggttaagg caaaagcgct ctatcaaaat taatttatgt
     3301 tttcaaaaga ctgtatctaa gaatgtatct gttatatgct gttatgaagt ggatttgagt
     3361 aaattgaaac ttatgaaata catttcataa tattaggtaa ctatcggtaa tataaagcac
     3421 ttatgaatat cggaatcacg gtttatccaa cttacggcgg aagcggaatt gtcggttcgg
     3481 aactcggtcg cgaattggcg ctgcgcggtc ataacgtcca ttttatctcg tcgatgctgc
     3541 cgacgcgcct gaccgagctt aacgagcgcg tgcattttca cgaggtcgag atgatgagct
     3601 acccgctttt cgagcatcag ccttatgatt tggcgctggc gacgaaaatg gcgaccgtcg
     3661 cgcgctcgga aaaactggat ttgctgcacg tgcattacgc gattccgcat tccatcagcg
     3721 cgattctggc gcgcgaatca atcaagcaaa agcgttacgt tccggttatc acgacgctgc
     3781 acggaaccga tattacgctg gtcggcgccg accgcagcta tctgccgatt acgcgttacg
     3841 gattgcagca atcggacggc gtgaccgccg tgtccaaatt tctgcaaaaa gcgaccatcg
     3901 aaactttcga tttcgacgaa atcgaagtca ttccgaattt tatctgcggc tgtcattaca
     3961 agcatctgcc ggaatcgcct ctgcgcgacg agctggcgcc gaacggcgaa aagcttctcg
     4021 ttcacgtttc caattttcgc gccgtcaagc gcccggtcga ttgcatcgag attctcgcga
     4081 aagtaaagga aaaaggcggg aaagcaagat tgattatggt cggcgacgga cccgaaaggt
     4141 cggcttgtta ttatcgcgcc gagcagttag gcgttaaaga cgacacggtt tttgtcggca
     4201 aacaggcgaa tattcccgat tatctcggca tcgccgacgt ttttctgctg ccctccgagc
     4261 tggaatcttt cggcttggcg gcgctcgaag ccgaagcctg cgaagttccg gtcatcgcga
     4321 cgcgcatcgg cggcattccc gaagtcgtca gcgacggcga aaccggattc ttaagcgaca
     4381 tcggcgacac ggaaaaaatg agcgacgacg tgatgaagtt tttaaacgac gaggaaatgc
     4441 ggcacagttt cggcgaaaaa gcgcgcgaat ccgccgtctc gcgctacggc tcgcagctta
     4501 tcatcccgca atatataaat ttctatgaaa gaattctcgg caaaaccaaa tcagccgccg
     4561 tttaaagacg cggaaaaccc ttcagccgaa aagcccgatg ctttctggta tcgcatttat
     4621 gcagcggtta tcgttacgac aattctggta atcgctgctc tttgggcgtt ttcgcgttat
     4681 ttttcaaaat aaaatgaaat atctcgattg gataatcgtc attgcctatc tggtttacgt
     4741 gatttgggac ggaatccgcc tgaccaagca cagcaacacc agcgatggtt attttctcgc
     4801 cagccgcagc ctgccgtggt gggcggtcgg gctttcggtg atggcgacgc agctttcggc
     4861 gataacgctg gtcggcacga ccgggcaagc ctactcggac ggaatgcgtt ttatccagtt
     4921 ttattacggc ttgccgctgg cgatgataat tttgtgcgtg acggtcgtgc cgttttttca
     4981 ccgcgcgaaa gtctatacgg cttacgaata tctggaaaaa cgctttgacg tgaaaacgcg
     5041 cgctttgacg agctttttct ttttgatttc gcgcgggctc ggcgtcggca caatcatttc
     5101 cgcgccttcg attgtgcttt ccatcgtctt cgggtggaat ttgattttca cgattttcgc
     5161 catcggaatg tcgaccaccg tttacacggt tttcggcggc gtgcaagccg tcacctggac
     5221 tgatgtcaag cagatgttcg tgattttttt cggcttggcg gtttgtttga ttgtgatttt
     5281 gtcgagtttt cccacaggag tt
//
LOCUS       DASWNE010000053         6898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_319166, whole genome shotgun sequence.
ACCESSION   DASWNE010000053 DASWNE010000000
VERSION     DASWNE010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6898
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319166"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..780
                     /locus_tag="VGB68_01160"
     CDS             7..780
                     /locus_tag="VGB68_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047864.1"
                     /translation="MAKNNQQFLLNFANPSPGVIIAVGGLIVFVLFLIGLTIALAARY
                     VEPSPQFGTLVLLAIVPLFGLILATAIILRNVRRLSRNPKEDFGELMLPEAQRRKLNA
                     EVRELAALIGIEKEQMGDLRAAYILAEDLALRQIEQEKKLPLKRHFRIGKAEFDAVFV
                     SRDVSTLVAVTFLVTSNISQKKIDEILSKVEEVKRKFARIKSKTKVRLMLILVTQMSA
                     EDEAKLRAAVATQFRSMAIDFAEIRLFDFEGLQRIYTED"
     gene            897..1658
                     /locus_tag="VGB68_01165"
     CDS             897..1658
                     /locus_tag="VGB68_01165"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HEY0047865.1"
                     /translation="MLENKVGMIFGVANQRSIAWACAEACAAQGAKLAFTYQGERLKE
                     KVEKLSGQTDDALVVPCDVTNQAEVDAAFEAVGKKYGKLDFLVHSIAFAPREALEGEF
                     LTTTRDAFLTALEISAFSLTQLALAALPLMKDGGSIVTMSYYGAEKVVMNYNVMGVAK
                     AALEASTRYLAADLGKHNIRVNAISAGPIQTLSARGVKGMGSLFSYVGEKSPLKRNVE
                     AREVGNTALFLVSDLSSAITGEVIYVDCGYNIMGI"
     gene            1739..2536
                     /locus_tag="VGB68_01170"
     CDS             1739..2536
                     /locus_tag="VGB68_01170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEY0047866.1"
                     /translation="MADTKANKPRIIKDITKPTEPETVAEAKELEADSYWQVTEDEVL
                     LRSPDPQDEFKSSDSWRVFRILGEFVGGFDSLATVTHGVTVFGSARTREDDPNYKAAY
                     ETGKLLAEAGFEVITGGGPGIMEAANRGAFEARGKSIGCNIELPFEQAANPYQTKSLT
                     FKYFFVRKTMFIKYSNAYIIFPGGYGTMDELFEALTLIQTRKIRNFPVVLFNSQYWRG
                     MLAWLTSMMLNEKNISPEDLSLMHLTDSPKDAVDFIIKCCGFDKKGE"
     gene            2594..2950
                     /locus_tag="VGB68_01175"
     CDS             2594..2950
                     /locus_tag="VGB68_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047867.1"
                     /translation="MAEQETFPYLSRYMNCISKKIPEIESEREKLKNFALAGLFLTYR
                     AFNHSGDTMTTEIENTLSESIHLKRLKAFKEYFGIKIMNTEHYFSMLNLSKFDLILMM
                     FRLSNLSRKLYNFVVK"
     gene            complement(3017..3331)
                     /gene="cutA"
                     /locus_tag="VGB68_01180"
     CDS             complement(3017..3331)
                     /gene="cutA"
                     /locus_tag="VGB68_01180"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEY0047868.1"
                     /translation="MLVVLTTTPNTEEAESLARKIVKARLAACVQVLPPTKSFYFWEN
                     EVQADSEHLLLIKTLPEKFDALEDFIQANHSYSVPEIVALPAEKVSESYLGWMKDYLM
                     EQ"
     gene            complement(3457..4440)
                     /locus_tag="VGB68_01185"
     CDS             complement(3457..4440)
                     /locus_tag="VGB68_01185"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY0047869.1"
                     /translation="MAKKNTENQGEAIEPSNNIRCRYCGQKNQVREGYQNNQAVCGRC
                     RLPLSDEPHKKFADLSKHDYVHPADSKALAALRAIPGIDTALKKLLAVTGESAIRVSF
                     MASAVRVTPKQCPDLHAKLQIACTTLGVDMPELYVQQNPIVNAFTGGVEKPIIVLHSA
                     LIERLTDEETLAVIAHEVGHIHAEHVLYLTAARLLEAIANLSVARLIPGSEIIKFLIS
                     AGISGALLAWARRAELSCDRAAMLVCQDEHVIGRTMMKLCGGTFASKIDYDLFLEQAK
                     EFQKNYDEKALDRFWADLINAGLSHPFPIWRVAEILKWVEEGEYDEVMEKN"
     gene            complement(4666..5859)
                     /locus_tag="VGB68_01190"
     CDS             complement(4666..5859)
                     /locus_tag="VGB68_01190"
                     /inference="COORDINATES: protein motif:HMM:NF022623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HEY0047870.1"
                     /translation="MSHNFKLAHRAFVFLLFVFAAQSSLAQELYEMPAGVESRWASGE
                     NPTGEKGKGGQTNGGRKGAPTVQIKAGESRVLAEAGNASGAVRRIWMTFPDRSPQMLR
                     SLRIDMYWDGATKPAVSAPLGDFFGVGLGQTATFQSALFSNPEGRSFNSMIPMPFKTG
                     MKIVMTNESKTNLDELFYDVNYTLGDRHAANMLYFHAYYRRENPTTLQKDYEILPQVS
                     GRGRYLGANIGVIVNTKEYFNTWWGEGEIKMYLDGDRAFPTIAGTGAEDYIGTAWGQG
                     QYANFYQGSPIADEKTMRWSFYRYHIVDPIYFYRDARITMQQIGYLAEHSAAPFIEKK
                     ITLYRAGPGLVPLDITRDRKTVNPADWKFERTDDWSSCVYFYLDKTTNNLPPIDPVEK
                     RIAGL"
     gene            complement(5995..6798)
                     /locus_tag="VGB68_01195"
     CDS             complement(5995..6798)
                     /locus_tag="VGB68_01195"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY0047871.1"
                     /translation="MDEGRYNRTEIEEFTREAMRDGFCVLPEHFSRQKLTGWREKFAP
                     LLEEHIRREGELQNRGAGRYYVTLPFDAPFADEEIFADADVLAIVENLVGRDFTMVQL
                     ATDTPLLGSDYQETHRDAPPLFPEIRQETPSFQLAVNFPLVDVTPENGPFEVARGTHL
                     VTKEDGLKMIETGERPLEPICLKLGDVMIRDVRGLHRGTPNKTREPRPMCVIGYSRRW
                     LFRPEVSIRIPRAVWENLSPHSQQMLRFNRLVESLADAPETEIYQAFAY"
BASE COUNT         1704 a         1760 c         1730 g         1704 t
ORIGIN      
        1 tgagagatgg caaaaaacaa tcaacaattt ttgctgaatt tcgccaatcc atcgcccggc
       61 gtgataatcg cggtcggcgg attaatcgtt tttgtccttt ttctaatcgg actgaccatc
      121 gcgctggcgg cgcgatacgt cgagccgtcg ccgcagttcg gcacgctcgt tttgctggct
      181 atcgtcccgc ttttcggttt aattttagcg acggcgatta ttctgcgaaa cgtccgccgc
      241 ctatcgcgca atccgaaaga agatttcggc gaattaatgc tgcccgaagc gcagcggcgc
      301 aagctcaacg ccgaagttcg cgagcttgcc gcccttatcg gaatcgagaa agaacagatg
      361 ggcgacctgc gcgcggcgta tattctggcg gaagatttgg ctttgcgaca aatcgagcag
      421 gaaaaaaaat tgccgctcaa gcgtcatttc agaatcggga aagccgagtt tgacgccgtt
      481 tttgtcagcc gcgacgtctc gacgctggtc gccgtcacgt ttctggtaac atcgaacatc
      541 tcgcagaaaa aaatcgacga gattttaagc aaagtcgaag aagtgaagcg aaaatttgcc
      601 cgcatcaaat caaaaaccaa agtcaggctg atgctgattt tagtcacgca aatgagtgcc
      661 gaagatgaag ccaaactgcg cgctgcggtg gcgacgcagt tccgctcgat ggcgatagat
      721 ttcgccgaga ttcgtttgtt tgattttgaa ggattacaga gaatttatac cgaggactga
      781 aaaaactgaa aatgtgaaaa tgtgagagcc tgaaaaagtt ttcgcccgca gccgcgcttg
      841 tgatttacag ctaagcctgt ttttacgctt ttacactttt tcacttttga atatttatgt
      901 tagaaaacaa agttggaatg attttcggcg ttgccaatca gcgttcaatc gcgtgggcgt
      961 gcgccgaagc ctgcgccgcg cagggcgcta agctcgcttt tacgtaccag ggcgagcgtc
     1021 tgaaggaaaa agtcgaaaag ctgtccgggc aaacggacga cgcgctggtc gtgccgtgcg
     1081 acgtgacgaa tcaggcggaa gtcgacgccg cgtttgaagc ggtcggcaaa aaatacggca
     1141 agctcgattt tctcgttcat tcgattgcat ttgcgccgcg cgaggcgctc gaaggcgagt
     1201 ttttgacgac gacgcgcgac gcctttttaa cggcgctgga aatcagcgcg ttttcgctga
     1261 cgcagttagc gctggcggcg ctgcctctga tgaaagacgg cggaagcatc gtgacgatga
     1321 gctattacgg cgcggaaaaa gtcgtaatga attacaatgt gatgggcgtc gcgaaagccg
     1381 cgctcgaagc ttcgacgcgt tatctcgccg ccgatttggg caaacacaac attcgcgtca
     1441 acgccatcag cgccggaccg attcagacgc tttcggcgcg cggcgtcaaa ggtatgggct
     1501 cgctcttcag ctatgtcggc gaaaaatcgc cgctcaaacg caacgtcgaa gcccgcgaag
     1561 tcggcaacac cgcgctgttt ctggtcagcg acctttcatc ggcaatcact ggagaagtaa
     1621 tctacgtcga ttgcggctac aatattatgg gaatctagga aatgcggaat gatgaatgcg
     1681 gaacgcggaa tgaaaaccgc tgttccgcgt tccgcgttcc gcgttccgca tttaaaaaat
     1741 ggcagataca aaagcaaata aaccgagaat aataaaagac attaccaaac cgaccgaacc
     1801 ggaaacggtc gccgaagcca aagagctgga agcggattct tactggcaag tcaccgaaga
     1861 cgaagtgctg cttcgctcgc ccgacccgca ggacgaattt aaaagctctg attcgtggcg
     1921 cgtttttcgt attttgggcg aattcgtcgg cggattcgat tcgctggcga ccgtgacgca
     1981 cggcgtgacg gttttcggct cggcgcgcac cagggaagac gacccgaatt ataaagccgc
     2041 ttacgaaacc ggaaaactgc tggcggaagc cggtttcgaa gtcatcaccg gcggcggtcc
     2101 cggcattatg gaagcggcaa atcgcggcgc gttcgaagcg cgcggaaagt caatcggctg
     2161 caacatcgag cttccgttcg aacaggctgc caacccgtac cagacaaaat cgctgacctt
     2221 taaatatttc ttcgtccgca agacgatgtt tatcaaatac agcaatgcct atatcatttt
     2281 ccccggcggc tacggcacga tggacgagct ttttgaagcc ctaacgctga ttcagacgcg
     2341 aaaaatccgc aattttcccg tcgtcctgtt caattcgcaa tactggcgcg gaatgctggc
     2401 gtggctgaca tcgatgatgc tcaacgaaaa aaatatcagc cccgaagatt tgagcctgat
     2461 gcatctgacc gattcgccga aagacgccgt cgattttatt attaaatgct gcgggtttga
     2521 taaaaaagga gagtaagcag tattcgcaaa gatgcattca tccagggaaa ttatcgaaat
     2581 attttcatat agtttggcgg agcaggaaac ctttccctac ctttctcgct atatgaattg
     2641 tattagcaaa aaaattccgg aaattgaaag cgaacgcgag aaactcaaga actttgctct
     2701 ggcaggtttg tttttgacgt atagagcctt taatcacagc ggcgacacaa tgacgaccga
     2761 gattgaaaat actttatctg aaagcattca tttaaagaga ttaaaagcat ttaaggagta
     2821 tttcgggata aagataatga ataccgagca ttatttttct atgctcaatc tttctaaatt
     2881 cgatttgata ttgatgatgt ttcggctgag caacctttcg agaaaacttt ataattttgt
     2941 tgttaaataa tatcaattat aaatttcgcg cccttaatta aggctctcgg aaatcggcga
     3001 tttccgagag ccttaattac tgttccatca aataatcttt catccagccg agatagcttt
     3061 ccgagacctt ttccgccgga agcgcgacga tttccggcac gctgtaactg tgattggctt
     3121 gaatgaaatc ttcaagcgcg tcaaattttt cgggcagcgt ttttattaaa agcagatgct
     3181 cggagtcagc ctgaacctcg ttttcccaaa agtaaaacga cttcgtcggc ggcaaaacct
     3241 gcacgcaggc ggcgagtctg gctttgacga tttttcgcgc cagcgattcg gcttcttcag
     3301 tattcggtgt tgtggttaaa acgaccagca taacaaaagt tgacaggaag ttgcggatag
     3361 ttgcaggaag ttgcggatag ttaaaaacag tttcggagtt tcaatcttcg acaagcatct
     3421 taactatctg caacttcctg caactatctg ttaactctaa ttcttttcca tcacttcgtc
     3481 gtattcgcct tcttcgaccc attttaaaat ttcggcgacg cgccaaatcg gaaacgggtg
     3541 cgaaagtccg gcattgatga ggtcagccca gaaacggtcg agagccttct cgtcgtagtt
     3601 tttctgaaac tctttggctt gctccaaaaa caaatcgtag tcaatcttgg aggcgaaagt
     3661 tccgccgcaa agtttcatca tcgttctgcc gataacgtgc tcgtcctgac agaccagcat
     3721 cgccgcgcgg tcgcacgaaa gctcggcgcg ccgcgcccag gcgagcaaag cgccgctgat
     3781 tccggcggaa attaaaaatt tgataatttc cgaaccggga atcaaacgcg cgaccgaaag
     3841 attggcgatg gcttcgagca agcgcgccgc cgttagatac aagacgtgct cggcgtgaat
     3901 atgaccgact tcgtgcgcga tgacggcgag agtttcctcg tcggtcaggc gttcaatcaa
     3961 agccgagtgc aaaacgataa tcggcttttc gacgccgccc gtaaaagcgt tgacaatcgg
     4021 attttgctga acgtaaagct ccggcatatc gacgccgagc gtcgtgcagg cgatttgcag
     4081 tttggcgtgc aaatccgggc attgtttcgg cgtgactctg accgcgctcg ccataaacga
     4141 aacgcgaatc gccgattcgc ccgtcaccgc cagaagcttt ttcaaagccg tgtcgatgcc
     4201 cggaatggct cgaagcgcgg cgagcgcctt gctgtccgcc gggtgaacgt agtcgtgttt
     4261 gctcaaatcg gcaaactttt tgtgcggctc gtccgacaga ggcagccggc agcgaccgca
     4321 aaccgcctga ttgttttgat agccttcacg aacctggttt ttctgcccgc agtagcggca
     4381 gcgaatgtta ttgctcggct caatcgcctc gccctgattt tcggtattct ttttcgccat
     4441 atttcaaaag tttaatttag atgcagccga taattcaaac ccgtaaacct gaacgaggcg
     4501 cgccggtaaa aagtttagtt tttgtaattc aaagatattg cccgcgaaac gcgccgaacg
     4561 gacgaaaaaa aggaagctgc aaatattttg tttaaaaaat tttccttctt ttcatccgtt
     4621 cggcgcgttt cgcgggcaaa ttcccttatc agatggattt tatatttata atcccgcaat
     4681 tcttttttcg accgggtcga tcggcggcag attattcgtt gtcttgtcca gataaaaata
     4741 aacgcagctc gaccaatcgt ccgtgcgctc gaacttccag tcagccggat tgaccgtttt
     4801 tctgtctctg gttatgtcca gcggcacaag cccgggaccg gcgcggtaaa gcgtgatttt
     4861 cttttcgata aacggcgcgg cgctatgttc ggcgagataa ccgatttgct gcatcgtgat
     4921 gcgcgcgtcc ctgtaaaaat aaatcgggtc gacgatgtga taacgataaa agctccagcg
     4981 catcgttttt tcgtccgcaa tcggcgagcc ttgataaaaa ttcgcgtatt gtccctgtcc
     5041 ccaagccgtg ccgatataat cctcggctcc ggttccggca atggtcggaa acgcgcggtc
     5101 gccgtccaga tacattttta tttcgccctc gccccaccac gtgttgaaat attctttcgt
     5161 gttcacaatc acaccgatat ttgcgcccag ataacgacct ctgcccgaaa cctgcggcaa
     5221 aatttcgtaa tctttttgca gcgtcgtcgg attttcgcgg cgataatacg cgtgaaaata
     5281 aagcatattc gcggcgtggc ggtcgcccaa agtgtaattt acgtcgtaaa aaagctcgtc
     5341 cagattcgtt ttgctttcgt tggtcatcac gattttcata ccggttttaa aaggcatcgg
     5401 aatcatcgag ttaaagcttc tgccttccgg attggaaaaa agcgcggatt gaaaagtcgc
     5461 cgtctgaccg agtccgacgc cgaaaaaatc gccgagcggc gcgctgaccg ccggtttcgt
     5521 cgcgccgtcc caatacatat caatccgcag gctgcgcagc atctgcgggc tgcggtccgg
     5581 aaaagtcatc cagattcgcc gcaccgcgcc gctcgcgttt ccggcttcgg ctaaaactct
     5641 ggattcgccc gctttaatct gtacggtcgg cgcgcccttt cgcccgccgt ttgtctgccc
     5701 gccttttccc ttttcgccgg tcggattttc gccgctcgcc cagcgcgatt cgacgcccgc
     5761 cggcatctcg taaagctcct gcgccagaga agattgagcg gcgaaaacaa acagcaaaaa
     5821 cacgaatgcg cgatgagcta atttgaaatt atgcgacata aagttttctc caaacgtaaa
     5881 aatgagctga taactgatag ttgattactg ataactgata gttgattact gataactgat
     5941 aatagctgtc agccatcagt ttttaattct cagttatcaa ctatcagtta tcagttaata
     6001 agcgaatgcc tgataaattt ccgtttcggg cgcgtccgcg agcgattcga cgaggcgatt
     6061 aaaccggagc atctgctgcg agtgcggcga gagattttcc cagaccgcgc gcggaatacg
     6121 tatcgagact tccggtcgga aaagccagcg tcggctgtaa ccgataacgc acatcgggcg
     6181 cggctcgcgc gttttattcg gcgttccgcg atgcagcccg cgcacgtcgc gaatcatcac
     6241 gtcgcccagt ttcagacaaa tcggctcaag cggtctttcg cccgtttcaa tcattttcaa
     6301 gccgtcttct tttgtcacca gatgcgttcc ccgcgcgact tcaaaaggtc cgttttccgg
     6361 cgttacgtcg acgagcggaa aatttacggc gagctgaaaa gacggcgttt cctgccggat
     6421 ttcgggaaac agcggcggcg cgtcgcggtg agtttcctgg taatccgaac ctaaaagcgg
     6481 cgtatcggtg gcaagctgaa ccatcgtgaa atccctgccg accagatttt cgacaatcgc
     6541 cagaacatcg gcgtcggcga aaatttcttc gtcggcaaac ggcgcgtcga acggcaaagt
     6601 cacgtaataa cgcccggcgc cgcgattctg caattcgcct tcgcgcctga tatgttcttc
     6661 gagcagcggc gcgaattttt cacgccatcc agtcaatttt tgtcttgaaa aatgttccgg
     6721 caatacgcag aagccgtcgc gcatcgcttc ccgggtaaat tcttcgattt cagtccggtt
     6781 ataacgcccc tcgtccataa ttattcttcc tttaataaat gaaaaatcac ttttccttta
     6841 ctaaatgata gcggtcggcg gacggatttt ccagcacctg aattttgccg ctcgtcca
//
LOCUS       DASWNE010000054         7097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_638507, whole genome shotgun sequence.
ACCESSION   DASWNE010000054 DASWNE010000000
VERSION     DASWNE010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7097
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638507"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="VGB68_01200"
     CDS             <1..942
                     /locus_tag="VGB68_01200"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEY0047872.1"
                     /translation="LTRIPGSGMSGRVTKKDILSFIESGAALKPQDLLRKDAPVAPQI
                     TPAAQPQTKTGTFPEVRGIEPAQRTSYTAPQVAASVGDRIEPMSVMRRKIAEHMTFSK
                     QTSAHVTSVYEIDMTSVARFRERNKAEFQSRYGTKLTFMPFIFQATTAALRKFPVVNA
                     QVSGEQVIYKGDINLGMAVALDWGLIVPVIKRADSLSLSGLATTANDLADRARTKKLM
                     PDEVAGGTFTITNPGVFGGLFGTPIINQPQVAILCVGTIEKRPKVITTPEGDDVIAIR
                     SMAYFALTYDHRIVDGADAEKFLSFMKQYLETTQFSL"
     gene            1483..2463
                     /locus_tag="VGB68_01205"
     CDS             1483..2463
                     /locus_tag="VGB68_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY0047873.1"
                     /translation="MLKKFSVLVLALLLGTASAFAQTDAQLRTVASGQKMKVQGVVVS
                     KDADRIIVRDATGVDTPVTLTNATSIKTKGGFFGGGDRVAVNQIVRGLNLEVEGRGDG
                     ANLVASKVRFGKDDFRVAQSIDTRVSPAEERITQAEQNAQRISGQIDELMAISNAARG
                     GAKAAQETADAAIQGVNATNSRITALDDYVVQSTASVNFKVGSAILSPEAKTQLDEVA
                     TAALTLKGYTIEITGFASAEGGEKMNKALSQRRAQAVIDYLVETHNVPLRRIGTSYGF
                     GELQAVADNTTREGRAQNRRVEVKLLVSRGLNQNVEVRKDTTETQPNDQQ"
     gene            2552..3670
                     /locus_tag="VGB68_01210"
     CDS             2552..3670
                     /locus_tag="VGB68_01210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0047874.1"
                     /translation="MKQGQLKVSFGILGLILILAFSVFAQTPQATPPPPVEDDDVIRV
                     ESRLVIVPVSITDKLGQPVLNLKKEEFFVSEENKPQEIAQVSDAEKVPLEIALLFDIS
                     ASTDAMFKFEQETAAKFLQDVMRQDDRATIFTIGGRPMMIQARDTAEKSAYSIKMIMP
                     SKQYTAFYDTVAAAADYLRAAPQGRRKVIVVISDGEDTNSVRIAEAIQDGYKSLGKKI
                     DSLDTKSLYQFTVRRRNEATIREQTRVLQSLQNADAVFYSINPAGSSYQLNKISQFGQ
                     SNLQRFADETGGAAFLPKFQPIDLKDGYQNTANIRLNQETLTKIFRQLANELQAQYLV
                     QYYSESDFPNNKYVKLNVGLKNPQNFRVRARAGYFVKK"
     gene            complement(3890..4450)
                     /locus_tag="VGB68_01215"
     CDS             complement(3890..4450)
                     /locus_tag="VGB68_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047875.1"
                     /translation="MAKFTSPLNNLKVASPCSQDWEAMIGSERKRFCGECKLNVYNLS
                     GMTRTEAENLLINSEGRLCVRFYKRADGTVLTKDCPVGWQALKKRLSRTAAAFVSLLF
                     GLLSGLGLMSLRSQPREERLMGAVAYQPTPVPKQTPEYAEMGKIAVMGNFTVPTPKPK
                     ATPKDRRATVGRLAEDFQRERSGERQ"
     gene            complement(4634..6319)
                     /locus_tag="VGB68_01220"
     CDS             complement(4634..6319)
                     /locus_tag="VGB68_01220"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEY0047876.1"
                     /translation="MKVTIGQINTTNGDIEGNVAKIIEAIEKAKADKSDIIVFPEVVT
                     HGYTSFDWFQDRDIIERALEPLEKIIPATTGITAVVGTIRPNLEIDGKRLYNCAAVIH
                     DGKLLGFADKTLLPEYDVFDDPRYFEPSDVRKIFEIEGQKIGVVVCEDFWNDKTFWKE
                     RLYDVDPADELIMQGADLIVAINASPFNKGKIKLRCDMVAHRAKLQKKPIVFVNLVGG
                     NDGIVFDGASLIADEEGDIILQAAAFEEFVETVELDVHRPDARGITGDECETIRQALV
                     LGIRDYARKNRFTKAVLGLSGGIDSAIVAALAAEAIGGENLLCVMMPSPYSSEGSIKD
                     SEQLVKNLGCQSRIEPISGAFDVLLNQLHLHKPTKSGESLAGENMQSRLRGVILMAIS
                     NAEGRLLLSTGNKSELAVGYCTLYGDTNGGLAVLGDVLKTEVWQVARHINESAGREII
                     PNAIIDKKPSAELAPNQFDQDSLPPYELMDPILRLYFENKLSPEEIILAENDPKLVYE
                     VLNKVESPANEFKRQQLPPTIIISRNAIGVGRRRPITHKYRRVQSQGKAQIQA"
     gene            complement(6316..>7097)
                     /locus_tag="VGB68_01225"
     CDS             complement(6316..>7097)
                     /locus_tag="VGB68_01225"
                     /inference="COORDINATES: protein motif:HMM:NF013003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEY0047877.1"
                     /translation="TDRFTKAVAQLGDDKLEIRLGGMYALERIAKDSPKDHWTVMEIL
                     SAYIRENAKKKEEETSENKNSKKTANDKVDKPEGNTKVTTDIQAALTIIARRKIEQDS
                     KTDKIDLSGANLSKSNLSGAFLAGANLSGAHLGGADLSGVFLGGANLSGAFLVGADLS
                     KSILPVVDLSDTKLSFADLSGADLSGANLSGADLSGANLSGADLSVELENFTFEQLSN
                     AIINENTALPSDLESRKADLLEISKKNLERREKFLNESNKK"
BASE COUNT         1755 a         1793 c         1704 g         1845 t
ORIGIN      
        1 ttaacgagaa ttccgggaag cggtatgagc gggcgcgtga cgaaaaaaga tattttgtct
       61 tttatcgaat cgggcgcggc tctgaaaccg caggatttgc tgcgcaaaga cgcgccggtc
      121 gcgccacaga ttacgcccgc cgcgcagccg caaaccaaaa ctggaacatt tcctgaagtc
      181 agaggcattg agcctgcaca gcgcacttca tacaccgcgc cgcaggtcgc cgcgtcggtc
      241 ggcgaccgga tagagccgat gtcggtcatg cgccggaaaa ttgccgagca tatgactttc
      301 tcgaaacaga cttcggcgca cgtgacgagc gtttacgaaa tcgatatgac aagcgtcgct
      361 cgctttcgcg agcgtaacaa ggcggagttt caatcgcgtt acggcacgaa attgacgttt
      421 atgccgttta tatttcaggc gacgaccgcg gcgctcagaa aattcccggt tgtcaacgcg
      481 caggtctccg gcgagcaggt tatttacaag ggcgacatca atctcggaat ggcggtggcg
      541 ctggactggg gcttgattgt tccggttatc aaacgcgccg attcgctttc gctttccggc
      601 ttggcgacga cggcgaatga tttggctgac cgcgcgcgaa cgaaaaaatt aatgccggac
      661 gaagtggcgg gcggaacgtt tacgattacc aatccgggcg ttttcggcgg gcttttcggg
      721 acgccgatta tcaaccagcc gcaggtggca atcctgtgcg tcggcacaat cgaaaaacgc
      781 ccgaaagtca tcaccacgcc ggaaggcgac gatgtcatcg cgattcgctc gatggcttat
      841 ttcgctttga cttacgacca tcgaatagtt gacggcgcgg acgcggaaaa attcctttcc
      901 tttatgaagc aatatctgga aacgacgcag tttagtcttt aaataagcgg taaatgaaaa
      961 ggtggtgagt ggtaagggct ggtttctttc actcaccact ttccatttcc gagcaaaatt
     1021 cagaacttac cgcttactgc ttgccacttt taccactttc aaaatttttt tatttcgtaa
     1081 attaaatttt attgtagaat agagaagtcc gtcttcggac gtctgcattt tttttcaaaa
     1141 acaggaaaca ttttcaaaaa actgccgata acgctatgta tggctatgtt tgaaaataga
     1201 atccgacaac tcgaattttg aaaaagtctg cgcttcccgg aattatctat tcagttgtcc
     1261 ggtcggattt tagtgattca agcaaggtca cgtatagcgt aaatgctttg cgatttcaaa
     1321 cgtaaacgct ttaaataaaa gtgtttacag ttatcaaaaa atttctcttt gtgcgcccgc
     1381 gacaaaattt gaattttttg acaactttgc taaaaaaata aacatcgcac aagctgattc
     1441 gcaatcaaga aatcaaaaaa gataaaggaa gggtttattt ttatgttgaa aaaattttca
     1501 gtattagttt tagctttgct tttgggaacg gcgagtgctt tcgcacaaac ggacgcgcag
     1561 ctgcgcaccg tcgccagcgg gcagaaaatg aaggttcagg gcgtggtcgt cagcaaggat
     1621 gccgaccgca ttatcgtccg tgacgcgacc ggcgtcgata cgcccgtgac gctgaccaac
     1681 gcgaccagca ttaaaaccaa aggcggattc ttcggcggcg gcgaccgcgt tgccgttaat
     1741 caaatcgttc gcggtttgaa tctggaagtc gaaggtcgcg gcgacggcgc caatctggtt
     1801 gccagcaagg ttcgtttcgg caaagacgat ttccgcgtgg cgcaatcgat tgacacgcgc
     1861 gtttcgccgg cggaagagcg catcacgcaa gccgagcaga atgcacagcg catttcgggt
     1921 cagattgacg aactgatggc gatttccaat gccgcccgcg gcggcgcgaa agccgctcag
     1981 gaaacggctg atgccgctat tcagggcgtc aacgcaacca attcgagaat cacggcattg
     2041 gacgattatg tcgttcaatc gacggcgagc gtcaacttta aagtcggcag cgcgattctt
     2101 tcgcccgaag ccaaaacgca gctcgacgaa gtggcgacgg cggctttgac tctgaaaggt
     2161 tacaccatcg aaatcaccgg ttttgcttcg gcggaaggcg gcgaaaagat gaacaaagct
     2221 ctcagccagc gtcgcgcgca agccgtgatt gattaccttg tggaaacgca caacgttccg
     2281 cttcgccgta tcggaacttc atacggtttc ggcgagcttc aggcggttgc cgacaatacg
     2341 acccgcgaag gacgcgcgca gaatcgtcgc gtcgaggtca aattgctcgt gagccgcggc
     2401 ttgaatcaaa acgtcgaagt cagaaaagat acgaccgaga ctcaaccgaa cgaccagcag
     2461 taattgctga aacaatcgac cgcagtagaa cgcgagagat ttttatcttt cgcgttctat
     2521 cgcggtattt ttttcaaggg ggcaaactgt catgaaacaa ggacaattaa aagttagctt
     2581 tggaatttta ggattgattt tgattctggc gttttctgtt tttgcgcaga cgccgcaggc
     2641 gactccgccg ccgccggtcg aagacgatga cgttattcgc gtcgaatcgc gcctggtcat
     2701 agttccggtt tcaatcaccg acaagctcgg tcagccggtt ttgaatctca aaaaagaaga
     2761 gttctttgtc agcgaggaaa ataagccgca ggaaatcgcg caggtatcgg atgcggaaaa
     2821 agttccgctc gaaatcgctc tgcttttcga tatttcggca agcacggacg cgatgttcaa
     2881 attcgagcag gaaactgccg ccaagttttt gcaggacgtg atgcgtcagg acgaccgggc
     2941 gacgattttt acaatcggcg ggcgtcccat gatgattcag gcgcgtgaca cggcggaaaa
     3001 atcggcttat tccatcaaaa tgattatgcc gtcgaaacaa tacacggctt tttacgacac
     3061 ggtcgccgcc gccgccgatt acctgcgcgc cgccccgcag ggcagacgta aggtcatcgt
     3121 cgtgatttcc gacggcgagg atacaaacag cgtgcgcatc gccgaagcga ttcaggacgg
     3181 ttataaaagt ctgggcaaga aaattgattc gctcgataca aaaagtcttt atcaattcac
     3241 cgtccgcagg cgcaatgaag cgaccatccg cgagcagacg cgcgttttgc aatcgctgca
     3301 aaacgccgac gcggtttttt attcgataaa tccggcgggc agctcttatc aattaaataa
     3361 aatcagccag ttcgggcaat cgaatctgca aagatttgcc gacgaaaccg gcggcgcggc
     3421 ttttttgccg aagtttcagc cgatcgattt aaaggacggc tatcaaaata cggcgaatat
     3481 ccgtcttaat caggaaactt tgaccaaaat ctttcgccag cttgccaacg aattgcaggc
     3541 gcagtatctg gttcaatatt attcggaatc tgattttccg aataacaaat acgtgaaatt
     3601 aaacgtcggc ttgaaaaatc cgcaaaattt ccgcgtccgc gcgcgggcgg gttattttgt
     3661 caaaaagtag aaacacaaaa gcggaaacgc gaaggtttaa aaccatgttt tcaaccttcg
     3721 cgtttccggc tcggtcgtat caaaccgaac tttgctcagg atttcccaat ccaaagccgc
     3781 attaaaattc atattataaa acggcggaac tcatctgccg tttttctttc ggctcgacga
     3841 gttccgcgcg tcgaaaacgg cgaatgacgt tcggagtctg gaaaagggat tattgtcgtt
     3901 ccccgcttct ttctctctga aaatcttcgg caagtcttcc gaccgtcgcc cgcctgtctt
     3961 tcggcgtcgc tttcggcttc ggagtcggaa cggtaaagtt tcccataacg gcaattttgc
     4021 ccatctcggc atattcagga gtttgtttcg gaacgggcgt cggctgatac gcgaccgcgc
     4081 ccatcaaacg ctcttctctc ggctggctcc tcagcgacat caagcccaat ccgctcaaca
     4141 aaccgaaaag cagcgaaaca aaagccgccg ccgttctgga aaggcgtttt ttgagcgcct
     4201 gccagccgac cggacaatct ttcgttaaaa ccgtgccgtc cgcgcgcttg taaaatctga
     4261 cgcagaggcg accttcggaa ttaatcaaaa gattttccgc ttcggttctg gtcatacccg
     4321 aaagattata aacattcagc ttgcactcgc cgcagaaacg cttgcgctcg ctgccaatca
     4381 tcgcttccca atcctgcgag cagggcgacg caacttttaa attattcagc ggactcgtaa
     4441 atttagccat aaaaactccg ttcaaaatta actcaggcgt ttgcctatgt ccttttgaac
     4501 ggagcgtgaa aatttttctt gcagaaaatt tttgaaaatt ttttattctt tcatttcctc
     4561 gggtgcgagc tgattttcag accttgcccg aagcgggttg aaaaaagcaa aaacaaaagc
     4621 aagccggcgg atttcacgct tgtatctgcg cttttccctg actttgaact ctcctgtatt
     4681 tatgcgtaat cggtcgccga cgcccgacgc cgatggcgtt tctggaaata ataatcgtcg
     4741 gcggaagctg ttggcgtttg aattcattcg ccggagattc gaccttgttc aaaacttcgt
     4801 aaaccagttt cgggtcgttt tcggcgagga tgatttcttc gggcgacaat ttattttcaa
     4861 aataaagccg caaaatcggg tccattagtt cataaggcgg caggctgtcc tggtcgaact
     4921 gattcggcgc aagctcggcg gaaggctttt tgtcgataat cgcgttcgga ataatttcgc
     4981 gcccggcgct ttcgttgatg tgacgcgcga cctgccagac ttccgttttc agaacatcgc
     5041 cgagaaccgc cagaccgccg ttcgtgtcgc cgtagagcgt gcagtaaccg accgccagct
     5101 ccgatttatt tccggtcgaa agcagcagac gaccttccgc attggaaatc gccatcaaaa
     5161 tcacgccgcg cagtcgtgac tgcatatttt cgcccgccag actttcgccg cttttcgtcg
     5221 gcttgtgcaa atgaagctgg ttcagcaaaa cgtcgaacgc gccggaaatt ggctcgatgc
     5281 gcgactggca gccgagattt ttgaccagct gctcggaatc tttaatgctg ccttcggacg
     5341 aatacggcga cggcatcatt acgcacaaaa gattttcgcc gccgatggct tccgccgcca
     5401 gagcggcgac aatcgccgaa tctattccgc ccgaaagtcc gagaaccgct ttcgtaaaac
     5461 gatttttccg cgcgtaatcc ctgatgccca aaaccagcgc ctgccgaatc gtttcgcatt
     5521 cgtcgccggt aattccgcgc gcgtccggac gatgcacgtc caattcgacc gtttcgacaa
     5581 attcctcgaa agccgccgcc tgcaaaataa tatcgccttc ttcgtcggca atcaggctcg
     5641 cgccgtcaaa cacaatgccg tcgtttccgc cgacgagatt gacgaagaca atcggctttt
     5701 tctgcaattt ggcgcggtgc gcgaccatat cgcagcgcag tttgattttg cctttgttga
     5761 acggcgaagc gttaatggcg acaatcaaat ccgcgccctg cataatcaat tcgtccgccg
     5821 ggtcgacatc atacaatctc tctttccaga aggttttatc gttccagaaa tcctcgcaga
     5881 cgacgacgcc gattttttgc ccttcaatct cgaaaatctt tcgcacgtcc gacggctcga
     5941 aataacgcgg gtcgtcgaaa acgtcgtatt cgggcagcag agttttgtcg gcgaaaccca
     6001 gcagttttcc gtcgtgaatg acggcggcgc aattgtagag gcgtttgccg tcgatttcca
     6061 ggttcgggcg aatcgtgccg acaaccgcag taattccagt agttgcggga attatttttt
     6121 ccagtggctc gagcgcgcgc tcgataatgt cgcggtcttg aaaccagtcg aacgaagtgt
     6181 agccgtgcgt gacgacttcg ggaaaaacga tgatgtccga tttgtcggct ttcgctttct
     6241 cgattgcctc gatgattttc gcgacgttgc cttcgatgtc gccgttggtc gtgttgattt
     6301 gtccgattgt tactttcatt ttttgttact ttcgttcaag aatttttccc ttctctcaag
     6361 attcttttta gagatttcaa gtaggtccgc ttttctgctt tctaaatcac tgggtagcgc
     6421 agtgttttca ttgataatcg cattgcttaa ttgttcaaat gtgaaatttt ccaattcaac
     6481 acttaaatct gcaccactta aatttgcacc acttaaatct gctccactta aatttgcacc
     6541 acttaaatct gctccactta aatctgcaaa acttaatttt gtatcactta aatccacaac
     6601 aggtaaaatt gatttactta agtctgcccc aactaaaaat gctccactta aatttgctcc
     6661 acctaaaaat accccgctta agtctgctcc acctaaatgt gctccgctta aatttgctcc
     6721 agctaaaaat gctccactta aatttgattt acttaaattt gcgccactta aatctatttt
     6781 gtctgttttt gaatcttgtt caatttttct tcgtgcaata atggttaaag cagcttgaat
     6841 atctgttgta acttttgtat ttccttccgg tttatcaact ttatcatttg cagttttttt
     6901 actgttttta ttctcggaag tttcttcttc ttttttcttt gcgttttcac gaatatacgc
     6961 cgaaagaatt tccatgactg tccaatggtc tttaggggaa tctttggcaa ttctttccaa
     7021 agcatacatt ccgcctaagc gaatctctaa tttgtcatcg ccaagttggg caaccgcttt
     7081 ggtgaagcgg tctgtta
//
LOCUS       DASWNE010000055         3170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_461639, whole genome shotgun sequence.
ACCESSION   DASWNE010000055 DASWNE010000000
VERSION     DASWNE010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461639"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1727
                     /locus_tag="VGB68_01230"
     CDS             <1..1727
                     /locus_tag="VGB68_01230"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0047878.1"
                     /translation="RPSFGVDIDTTGAFTKSERVFYRALFTLENMNYFTANTLDRNRY
                     ANASVSFKLDEAGRYVLTPSVQYTRYNRPYGGGIVISPSSSLSALAPSSLPASLNDPV
                     NAPINEDDLSPLDVNLFGGRRIEKTGWAGLDFRGVVSEKIRVNATYRYVSFDTDINSF
                     TPQVNQNSTAQINQLRNQNTVSRIQAKSLTERKYNNFNADAGYEWLNDGWWKNTTQIG
                     FYERVLDSRATAAQGAIPAAQSPINIYTGQTLSPLVDNFPPIAFGAWGRDIVWNGFVQ
                     NRTSLAGGRWNFTLGLNYGQNKPAPTAANPNPATRKSDLMPNASVVFNAAPEFTLYAS
                     YSTSFNPVDPDLEDAQGNRGTFAPTLGINYEIGAKYDLFNRRVSMTLALFQNQIDNAL
                     VQSDVNVLNPRGNRYYTPAGTRRARGAEMTGEFQVRQDLRISAGVSYLDAIYKGFPAT
                     AAVSSSPIPNSRAEKSPRWSYNIYSRYDRPEGFLKGFGAGLGLFWQGKRLGSNGAQTF
                     AAPDPLVLPSYTRVDSALFYRLNKHVNFAFNVENMFNELIYVNASVGSSIEIAAPRTM
                     TFRTTFNF"
     gene            1803..2969
                     /locus_tag="VGB68_01235"
     CDS             1803..2969
                     /locus_tag="VGB68_01235"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HEY0047879.1"
                     /translation="MKSFRKVLFWLHLTAGVATGIIIFIMCVTGVILTFEAQIKDYAE
                     SDVRYVAAPETGAQKLTPTELFTKLREAKPDAKPTGLTLKADRTASAAFALGREGALY
                     MNPYSGEILGGDAKAVNNFFRSVTDWHRWLGASGENRAVGKAITGFSNAAFLVLALTG
                     IYIWFPRRRAWRNFKAVMFFRRGLQGKARNFNWHNTIGFWSSLVLIILTVTGMVISYQ
                     WAGNLVYTLTGSEAPQQQAPPPTQNQPNRETNPVIPANLDELWNRAAQQTEGWEFITL
                     RVPTSAEAPVVFSIREGKELNPFAGSTATFNAQNGEITKWEPYSGNNAGRRLRSWMRF
                     AHTGESFGIVGQTVAGLASLGGAFLVWTGLSLAWRRFRNWRARKENNPEQLSSL"
BASE COUNT          690 a          881 c          880 g          719 t
ORIGIN      
        1 agcgcccgtc gttcggcgtc gatatcgaca cgaccggagc gtttaccaaa agcgagcgcg
       61 ttttttatcg cgctttgttt acgctggaaa atatgaatta ttttacggcg aacacgctcg
      121 accgcaaccg ctatgcaaac gcttcagttt cctttaaatt agacgaggca ggtcgctatg
      181 ttttaacgcc gagcgttcaa tacacgcgct acaatcgacc ttacggcggc ggcatcgtca
      241 tttcgccgtc ctcgtccctt tcggctttag ctccgtcgtc gttaccggcg tcattgaacg
      301 acccggttaa cgctccgatt aacgaagacg atttatcgcc gctcgacgta aatttattcg
      361 gcggcaggcg catcgaaaaa acgggctggg ctgggctgga ctttcgcggc gtcgtgagcg
      421 aaaaaattcg cgtcaacgcg acttatcgct acgtttcttt tgatacggac atcaattcct
      481 ttacgccgca ggtcaatcag aattcgacgg cgcaaatcaa ccagctccgc aatcaaaaca
      541 ccgtttcgcg gattcaggcg aaaagcctga ccgagcgcaa atacaataat tttaacgccg
      601 atgccggcta cgaatggctg aacgacggct ggtggaaaaa cacgacgcag ataggttttt
      661 acgaaagagt tttggattcg cgcgcgacgg cggcgcaggg cgcgattcca gccgcgcaat
      721 cgccgataaa catttacacc ggacaaactt tgtcgccgct cgtcgataat tttccgccga
      781 ttgccttcgg cgcgtggggc agagatattg tctggaacgg ttttgtgcaa aaccgcacgt
      841 cgctggcggg cgggcgctgg aatttcacgc tcggcttgaa ttacgggcag aacaaacccg
      901 cgccgacggc ggctaatcct aatccggcga cgcggaaaag cgatttgatg ccgaacgctt
      961 ccgttgtttt caacgcggct cccgaattta ccctttacgc cagttattcg acgagcttta
     1021 atccggttga cccggatttg gaagacgcgc agggaaatcg cggaactttt gcgccgacgc
     1081 tcggcataaa ctacgaaatc ggagcgaaat acgatttatt caatcgccgc gtttcgatga
     1141 cgctcgcttt atttcaaaat caaatcgaca acgcgctcgt ccagtcggac gtcaatgttt
     1201 tgaatccgcg cggcaatcgt tattacacgc ccgcaggaac gcgacgggcg cgcggcgcgg
     1261 aaatgacggg cgagtttcaa gtgcggcagg acttgcgaat ttcagccggc gtcagctatc
     1321 tggacgcgat ttacaaagga tttccggcga ccgccgccgt ttcgtcgtcg ccgattccga
     1381 attcgcgcgc cgaaaaatcg ccgcgctggt cttacaacat ttattcgcgc tacgaccgcc
     1441 cggaaggctt tctgaaaggc ttcggcgcgg gactcggctt gttctggcag ggaaaacgcc
     1501 ttggaagcaa cggcgcgcag actttcgccg cgcccgaccc gctggttttg ccgtcttaca
     1561 cgcgcgtcga ttcggctttg ttttatcgcc tgaacaaaca cgttaatttc gcgttcaacg
     1621 tcgaaaatat gtttaacgaa ctgatttacg tcaatgcttc ggtcggttcg agcatcgaaa
     1681 tcgccgcgcc gcgcacgatg acttttcgca cgacttttaa tttttaggcg gataagctgc
     1741 gggcggctaa ttgtcggaag cccggattgc gcccggtcgc ttttatttgt tctcatatat
     1801 atatgaaatc ttttcgcaaa gttttattct ggcttcattt aacggcgggc gtcgcgacgg
     1861 gaataattat ttttataatg tgcgtgacgg gcgtgatttt aacgtttgaa gcgcaaatca
     1921 aggattacgc cgaaagcgac gtgcgttatg tcgccgcgcc ggaaacgggc gcgcaaaagc
     1981 tgacgccgac cgaattattt acaaaattgc gcgaagccaa gccggacgcg aaaccgaccg
     2041 gactgacgct caaagccgac cggacggctt ctgccgcttt cgctctcgga cgcgaaggcg
     2101 cgctatatat gaacccgtat tcgggcgaaa tcctcggcgg cgacgccaaa gcggtcaaca
     2161 atttttttcg ttccgtcacc gactggcatc gatggctcgg cgcgagcggc gaaaaccgcg
     2221 ccgtcgggaa agcgataacg ggtttttcca acgcggcttt tctggttctg gcgctgacgg
     2281 gcatttacat atggtttccg cgccggcgcg cgtggcggaa ttttaaagcc gtgatgtttt
     2341 tccggcgcgg gcttcaggga aaggcgcgca atttcaactg gcacaacaca atcggcttct
     2401 ggtcgtcgct cgttctgata attctgaccg tcaccggaat ggtcatttcc tatcaatggg
     2461 cgggcaatct ggtttatacg ctgacgggca gcgaagcgcc gcaacagcag gcaccgccgc
     2521 cgacgcagaa tcagccgaac agggaaacga atcccgtcat tccggcgaat ctcgatgaat
     2581 tgtggaatcg cgccgcgcag caaaccgaag gctgggaatt tatcaccctg cgcgtgccga
     2641 ccagcgccga agcgcccgtt gttttttcga ttcgcgaagg caaggagctg aatcctttcg
     2701 ccggctcgac ggcgactttt aacgctcaaa acggtgaaat caccaaatgg gagccgtatt
     2761 cgggcaataa cgccggacgc cgcctgcgct cctggatgcg tttcgcacac accggcgaat
     2821 cgttcggcat cgtcgggcaa accgtcgccg ggctggcgtc gctcggcggc gcgtttctgg
     2881 tctggacggg actttcgctg gcgtggcggc gtttccgcaa ctggcgcgcg agaaaagaaa
     2941 ataatccgga gcagctttcg agtctttagg acaaatgcag gggcgcgcac ggcggcgtgc
     3001 tcctgcattc ctgtaattac tggtcgtaga gatataatcc cagaccaaac ttcgccgccg
     3061 cccgccgcag cgccatcgat tcggcgttgg agctggaatc gccgtaggaa gtcgtttcct
     3121 cttcttcctg cccggttgcc tcgcggtaaa taacgccttc ctggcaggga
//
LOCUS       DASWNE010000056         1918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_249425, whole genome shotgun sequence.
ACCESSION   DASWNE010000056 DASWNE010000000
VERSION     DASWNE010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249425"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VGB68_01240"
     CDS             complement(<1..257)
                     /locus_tag="VGB68_01240"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047880.1"
                     /translation="MVEVIIAMIILLVALLGVFLTFTYAVNYNAGNNSRAQALSILQQ
                     EVELLRSAKFTPGVTDDVLTGGTKTPKIITSADGSRFRVEIT"
     gene            complement(407..1042)
                     /locus_tag="VGB68_01245"
     CDS             complement(407..1042)
                     /locus_tag="VGB68_01245"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY0047881.1"
                     /translation="MKEIESNYQKGFSLLELLIVVVIIGIVSVLAMTQFGRTETRLQR
                     QNIARELKVYLERARFDSVKRRAVNPTDMARVIITSATAFNVTTDMNLNGTIDSADTR
                     FMSLNGRTDARITGNNLIFPIVIRFDHRGQATAIDNNGANVDPTFTICDANCTLATAN
                     ANNSTVITVTPSGTVAMMNGGSSLSNINAPNVTTIAGNSNISNTVYVNGNN"
     gene            complement(1238..>1918)
                     /locus_tag="VGB68_01250"
     CDS             complement(1238..>1918)
                     /locus_tag="VGB68_01250"
                     /inference="COORDINATES: protein motif:HMM:NF016032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HEY0047882.1"
                     /translation="YFLPHVLAMTTSSPFWLGFNTGLKSYRSEVFKKFPRTDIPDHFE
                     SFQQFQSYVDLLIKMNCIDNAKKIYWDVRPHPFFPTLEFRICDIPTRVDDTVAIAALF
                     QAIVAKLDTLIDKNLGFRLYHRRLIQENKWRAVRYGLDGKLLDLGKQKEVPVKDLIRE
                     LLDFVDDVVDDLNSRKEIEHVHTILERGTSADEQLRVYAEAGNNFAPVVDMLIKNTME
                     SVPEQCFD"
BASE COUNT          463 a          468 c          472 g          515 t
ORIGIN      
        1 gtaatctcga cgcgaaaacg gctcccgtcc gccgaagtta taattttcgg cgttttcgtt
       61 ccgcccgtta acacgtcgtc ggtcacgccg ggcgtgaatt tggcgctgcg cagcagttcg
      121 acttcctgct gcagaatcga aagagcctgc gcgcgcgaat tgtttcccgc gttgtaattg
      181 acggcgtagg taaaagtaag aaaaactccg agcagagcga ccagcaaaat aatcatcgcg
      241 atgatgactt cgaccaacga aaagccctct tcgctttttg ttttgttttt ttgaaccggc
      301 tcgtttgcgc tgtcagaagc acttttcttt ttacttttca tatattgggc tgataaagct
      361 tcctgaccgg acacttcggt cagttattca ttactaattg ccgttactaa ttgttgccgt
      421 ttacataaac cgtgttgctg atgttgctgt tgccggcaat cgtcgtcaca ttgggagcat
      481 tgatattcga gaggctggag ccgccgttca tcatcgcgac cgtgccgctc ggcgtaaccg
      541 taatgaccgt cgaattgttg gcgttcgccg tagcgagagt gcagttggcg tcgcaaatcg
      601 taaaggtcgg gtctacgttt gcgccgttat tgtcgatggc ggtcgcctgc ccgcgatggt
      661 cgaagcggat aacaatcggg aaaatcagat tgttgcccgt gatgcgggcg tcggtgcgac
      721 cgttcagact cataaaacga gtgtcggcgc tatcaatcgt cccgttcaga ttcatatcgg
      781 tcgtcacgtt aaacgccgtc gcgctggtta ttatcacgcg cgccatatca gtaggattaa
      841 ccgcgcgtcg cttgaccgaa tcaaaacggg cgcgctcaag ataaactttc agttcccgcg
      901 cgatattctg ccgctgcaat ctggtctccg ttcttccaaa ctgcgtcatc gccagcaccg
      961 atacgatgcc gataatgacg accactatca gcagttccag caatgaaaaa cctttttgat
     1021 aattcgattc aatttctttc atttaaatct cgcgttctgg aagaagttcg ggatgggtag
     1081 tacacagcac tactacaatt aataatttta cactattcga ggtaaactat gtcaacactt
     1141 gtttgcaaat gtttaacata ttattcatag aaaaagtcag gttgacgtta attgattcaa
     1201 cctgacttaa ctttttcaat ttgaccgagc cgtttagtta atcaaaacat tgttcgggca
     1261 cgctttccat cgtatttttg ataagcatat cgacgacggg cgcgaaatta tttcccgctt
     1321 cggcgtaaac gcgaagctgc tcgtcggctg acgtgccgcg ctccagaatc gtgtgaacgt
     1381 gttcgatttc cttgcgcgag tttaaatcgt cgaccacatc gtcgacaaaa tcgagcagct
     1441 cgcgaatcaa atccttgacc ggaacttctt tttgttttcc taaatcgagc agctttccgt
     1501 ccagcccgta acgcaccgcg cgccatttat tttcctgaat caagcggcgg tgataaagac
     1561 gaaaaccgag atttttatct attagcgtgt cgagcttggc gacgattgcc tgaaacagcg
     1621 cggcgatggc gaccgtatcg tcgacgcgcg tcggaatatc gcagatgcga aactccagcg
     1681 tcgggaaaaa cgggtgcggg cgcacgtccc agtaaatctt tttcgcattg tcgatgcagt
     1741 tcatcttgat aagcaaatcg acgtaagatt gaaactgctg aaaagattcg aaatggtcgg
     1801 gaatgtcggt gcgcggaaat tttttgaaaa cttccgagcg gtaggatttc aagcccgtgt
     1861 tgaatccgag ccagaacggc gaggaggtag tcatcgccag aacgtgcggc agaaaata
//
LOCUS       DASWNE010000057         3679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_213782, whole genome shotgun sequence.
ACCESSION   DASWNE010000057 DASWNE010000000
VERSION     DASWNE010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213782"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(644..1003)
                     /locus_tag="VGB68_01255"
     CDS             complement(644..1003)
                     /locus_tag="VGB68_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047883.1"
                     /translation="MKRKAIKQANNHSKYECLPPDVELETIVSATQVESAAGNKIVKE
                     TVKQRLDKINAGCRAGKLADGKGREIRFYRLQGCWGNPPQDYQEILNNQQKELEELKK
                     KYTVIEITCNPSGGMPF"
     gene            1653..>3679
                     /locus_tag="VGB68_01260"
     CDS             1653..>3679
                     /locus_tag="VGB68_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047884.1"
                     /translation="MSKEEYRHRRSEQIDQYRGMVDGDRGEKLRIRNREISKMEQQID
                     ELRAKPDSAQRDSLLAAWVPIGPDPIPNGQTQIVTTPVSGRVTAIDVHPTNPLIAYVG
                     TAQGGLYRTLDGGDTWTQLMQDAQSLAIGAVRIAPSQPETVYVGTGEPNFSTDSYFGV
                     GIYRIDNASTTAVKTGPIGNGNFNARSIAEILVHPTDPATIFVASASACAAINCGAAP
                     PAVIPAQNRGVWRSSNATSAAPTFAQLPFPFANQNLSVRDIVIDPQNPNLLVAWVVAN
                     GGGVLVSNNAMNADPATVTMVQRVTASGTATSDVNGELAITRPAGAPQPTIYVATGVA
                     GGTILRSSDGGVTFPLQIDNNFCTPQCFYDIAVEADPVDPLRVYIGGAPNIVFAFSTT
                     GGTAFTESETGLHVDSHVIRVAPSNPNIIYFGSDGGIYRSDTQGLAWIPKNNTGFLAT
                     QFMGLDNHPTDPNILIGGTQDNGTNRFTTAGVWTRTDFGDGGYSVIDQSSTDNVIYNQ
                     YHTYFNASNLTGYAFTNNGAAFENWTFRGCNSPPTVRNNITCTAVINFYAPLERGPGT
                     PNTIYYGSDRLYRSADTGVTHTTESQTFTSPLSAIGISPQNDLVRIIGLNNGGIFGTT
                     TGANPLVDMDPTNAVPAAYVTRTVISPTDVNTAYVTLALFNAPQVYRTNNL"
BASE COUNT          922 a          977 c          874 g          906 t
ORIGIN      
        1 cgatgacgtc agcaaaaaag caattaagcc cgcggctaag atttgttttg gcaacgagaa
       61 aagctcgtgg atatatttta atccattcga cgctgttact ttacttttgg gtaaactcat
      121 actaactcct tcaatagaac agggaaattc aattcttata tttagttgtg gggcgcaaca
      181 ttaaataaaa aaaacagttt tttatggata cgtaccttga aacctacgct ttggatattg
      241 aaagcgaccc ttgcagatta tttatggaaa taaaaggcgg aagataacaa cactaccgac
      301 aattttttgg tgggcaagaa tcggttaata cggaatagcg tttcccggcg aaatttttca
      361 aagcttcgtt gggcagacgt ttacttaaag atattaactg cctgaaagat aaactcatta
      421 gactgttttg tcaaataata tttgatggaa ataattataa gagccgacct ttacgcttac
      481 ttttcggagt tgaatcagcc gacaatttaa ccgtcgaaat agatttcagc aatcaaatta
      541 gaattcttta tctaatatta ttcatttcaa taaaaaaagg gcggctgacg aagctgccct
      601 tttttatttg cgcgtctaag ccagccggcg aatttgtctt ttattaaaaa ggcattccgc
      661 cggaaggatt acaggttatt tcgatgaccg tgtatttctt ttttaattcc tctaattctt
      721 tctgctggtt attcagaatc tcctgataat cctgcggcgg atttccccag cagccctgca
      781 accggtaaaa acgaatttcc ctgcctttgc cgtcggctaa tttaccggcg cggcatccgg
      841 catttatttt atccaaacgc tgtttgacgg tttccttcac gattttgttt cccgcagccg
      901 attctacctg cgttgccgag acaatcgttt cgagctcgac gtccggcggc aaacactcgt
      961 atttcgaatg attattcgcc tgtttaatcg ctttccgttt cattttggat ttactctgcc
     1021 cgtgaagcga ttgagcgctt ccggcaatta atataaatat aagtaaatga aaacaatact
     1081 ttatcatacg cgtcaaattg cctcctaaaa tctccgccga aaaatttgat gccgaaaata
     1141 gccgtgcggg attgaaattt tcgagcattt gctttagcat taatcagtta ccgataagcc
     1201 ttgacaatat ctcctacgag ataaaactct acaaaacatt cgccgcgaaa caaagttttt
     1261 tttatccggc gcggtgctca caattttcaa gagcagggcg aattaacgac tcaaagttaa
     1321 aaacagttga taaattaagg cgatattcaa aaaaagattg attttgcttt gattttgctt
     1381 tagcattttt taactacaca tttttcccaa tatagttttt ttgagcatac agtttataaa
     1441 agtcttgtgc catattcgag cgtgcagcgc tgaaattgtg tgcctagttt taggagaata
     1501 cgatgaaaaa tttttttacc ggaaggttga ttttgatcgc cttgggcttc attgtcgcag
     1561 cggtttggtt taatcccttc aatactactg ttgccaatta ctttaacgat gaccccgata
     1621 ttccagactt cgtgaagaaa gccaaatctc agatgagcaa agaggaatac aggcatcgcc
     1681 gctcggagca gattgaccag tatcgcggta tggttgacgg agaccgcgga gaaaaactca
     1741 gaatccgcaa ccgggaaatt tcgaaaatgg agcagcagat tgacgaactg cgcgccaaac
     1801 ccgattcggc gcagagagac tcgctgctcg cagcctgggt tccgattggt cccgacccga
     1861 ttccgaacgg tcagacgcaa atcgtcacca ctccggtttc aggtcgcgtg acggcaatcg
     1921 acgttcaccc gaccaatccg ctcattgctt atgtcggcac ggctcaaggc ggtctgtatc
     1981 gcacgctgga cggcggcgat acgtggactc agctgatgca ggacgcgcag agtttagcga
     2041 ttggcgctgt cagaatcgcg ccttcgcagc ccgaaacggt ttacgtcggc acgggcgaac
     2101 ctaatttttc gaccgacagc tatttcggcg tcggcatcta tcgcatcgac aatgcgagca
     2161 cgacggcggt caaaacagga cctatcggca acggaaattt caacgctcgc tccatcgccg
     2221 aaattctggt tcacccgacc gacccggcga cgatttttgt cgcatcggct tcggcttgtg
     2281 ccgccatcaa ctgcggcgcg gctccgccgg cggttatacc cgcgcagaat cgcggcgtct
     2341 ggcgctcaag caacgcgaca tcggcagcgc cgacattcgc gcagcttccc ttcccgttcg
     2401 ccaaccagaa cttaagcgtc cgcgacatcg ttatcgaccc gcagaatcct aatcttctgg
     2461 tagcctgggt cgtcgccaac ggcggcggcg tgcttgtttc caacaacgcg atgaacgccg
     2521 acccggcgac cgtgacaatg gttcagcgcg tgacggctag cggcacggcg accagcgacg
     2581 tcaacggcga attggcaatc acgcgcccgg caggcgctcc gcagccgacg atttacgtgg
     2641 cgacaggagt tgcaggcggc acaatactcc gttcgagcga cggcggcgtc actttcccgc
     2701 tgcagattga caacaatttc tgcacgccgc agtgtttcta cgacatcgcc gttgaagccg
     2761 acccggttga cccgctgcgc gtttatatcg gcggcgcgcc gaacatcgtt ttcgcctttt
     2821 caaccactgg cggaacggct ttcaccgaaa gcgaaaccgg cttgcacgtc gattcgcacg
     2881 tgattagagt cgcgccttca aacccgaaca tcatttactt cggcagcgac ggcggcattt
     2941 accgttcgga cacgcagggc ttagcctgga ttccgaaaaa caacaccggt ttcctcgcga
     3001 cacagtttat gggcttggac aatcacccga ccgacccgaa cattctgatc ggcggcacac
     3061 aggacaacgg cacgaaccgc tttacgaccg cgggcgtttg gacgcgcacg gacttcggcg
     3121 acggcggtta ttcggtgatt gaccaaagct cgacggacaa tgtcatctac aaccagtatc
     3181 acacgtattt caatgccagc aacctgacgg gctacgcctt cacgaacaac ggagcggcat
     3241 ttgaaaactg gacgttccgc ggctgtaaca gcccgccgac cgttcgcaac aacatcacct
     3301 gcacggctgt catcaacttc tacgcgccgc tcgaacgcgg tccgggaacg ccgaacacga
     3361 tttactacgg ctccgaccgt ttgtatcgct cggctgacac gggcgtgacg cacacgaccg
     3421 aaagccagac gttcacgtcg cctttgagcg ccatcggcat ctcgccgcag aacgacctgg
     3481 ttcgcatcat cggcttgaac aacggcggaa tcttcggaac gacgaccggc gcgaatccgc
     3541 tggttgatat ggacccgacg aacgccgtgc ccgccgctta cgtgacgcgc acggttatca
     3601 gcccgacgga cgtgaacacg gcttacgtca cgctggcgct tttcaacgca ccgcaggttt
     3661 accgtaccaa caatctgag
//
LOCUS       DASWNE010000058         2203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_531675, whole genome shotgun sequence.
ACCESSION   DASWNE010000058 DASWNE010000000
VERSION     DASWNE010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531675"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="VGB68_01265"
     CDS             complement(<1..772)
                     /locus_tag="VGB68_01265"
                     /inference="COORDINATES: protein motif:HMM:NF018699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
                     /protein_id="HEY0047885.1"
                     /translation="MNEAKALLDAGNLGGAVEAAISAVKSKPTDLQARTFLFELSLFS
                     GDWERANRQLDAIGHQSVEAMIGSKIYQQCVEAERKRAKFFSESQKPEFLSTPPDYVY
                     GLLTANNRLREGKTAEAREILDTVEEQRPAFACRIDGEEKQDFRDYNDLTSSILEVII
                     KDSYVWVPFEEIVKIEFTKPHSLRDLFWLQANLETLSGTNGEVFIPTLYVDSWKHASD
                     QVRLGRMTDWRDAGSELYVGEGQKLFWIDGEDRPILELS"
     gene            complement(842..>2203)
                     /locus_tag="VGB68_01270"
     CDS             complement(842..>2203)
                     /locus_tag="VGB68_01270"
                     /inference="COORDINATES: protein motif:HMM:NF045283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion IcmF C-terminal
                     domain-containing protein"
                     /protein_id="HEY0047886.1"
                     /translation="DEWRKFVKSVNVRSYANQAEADNALEKFASANSPMTALMREIMR
                     QTNLSGKPKPAGWLDWLTSFFQSKKKIETGGNSPVERDFRPLFAFVGTDEKPESSQIN
                     KYGLQIQSVYSNFHALGQDEFSQVTKDIAQQKDLKLEPKLRDAEKAISSMTGSFNDTP
                     AGQELANILKKPLTNLRNLLGVGVTNQLDKAWAEQIFPKAKEIEKGYPFEDAGEADIT
                     KLTAYLNPADGTFSKFFDERLAKYFEESNGQWKPKATSEVKFSDEFVVYLNNAFRLRE
                     ALFGKSKSPSFNYEFRLQKAGDALIEVTIDGQKIDSNGTGSTNFKFPAPSGDTGAFMK
                     FASTADASTTSGTTPAGNTSANSTPANVSNSGNSNTTSRFLQSPNTNSSGASSIERPG
                     TWGLFKFFEAGKPEKQSSGEYKLTYTLGGKTVTATVKPTGGDLFDRNMFKSLRAPQNL
                     LKQ"
BASE COUNT          441 a          626 c          559 g          577 t
ORIGIN      
        1 tgctcaattc cagaatcggt cgatcttcgc cgtcaatcca gaaaagcttc tgaccttcgc
       61 cgacgtaaag ctcgcttccg gcatcgcgcc agtccgtcat tctgccgagc cgcacctggt
      121 cgctcgcgtg cttccacgaa tcgacataca aagtcggaat aaaaacttcg ccgttcgtgc
      181 cgctcaaagt ttccagattc gcctgcagcc agaaaaggtc gcgcaagctg tgcggcttgg
      241 taaactcgat tttcacgatt tcctcaaacg gaacccagac gtaggaatct ttgataatca
      301 cttccagaat cgacgatgtt aaatcgttgt aatcgcgaaa atcctgtttt tcctcgccgt
      361 cgatgcggca ggcaaacgcc gggcgctgtt cttcgaccgt gtccagaatc tcgcgcgcct
      421 ccgccgtttt tccttcgcgc aggcgattat tcgccgtcag caagccgtaa acgtagtcgg
      481 gcggcgtcga aagaaattcc ggtttctggc tttccgagaa aaatttcgcg cgcttgcgct
      541 cggcttcgac gcactgctgg taaatcttcg agccgatcat cgcttccacg ctctgatgac
      601 cgatggcgtc gagctgccgg ttggcgcgtt cccaatcgcc cgaaaaaagc gaaagctcaa
      661 ataaaaatgt gcgcgcctgc aggtcggtcg gcttcgattt gaccgcgctt atcgcggctt
      721 cgaccgcgcc gcctaaattt cccgcgtcga gtaacgcttt agcttcgttc ataattttag
      781 ctctcagcaa tcagcaatca gctatcagcc aaacaactgc ttacggctca ccgctgactg
      841 ctcactgttt caaaagattc tgcggcgcgc gcagcgattt gaacatattt ctgtcgaaca
      901 aatcgccgcc cgtcggcttg acggtcgccg tcacggtttt gccgccgagc gtgtatgtta
      961 atttatattc gccgctcgat tgtttttccg gctttccggc ttcaaaaaac ttgaataagc
     1021 cccaggttcc cggtctttca atcgacgacg cgccggacga attcgtgttc gggctttgca
     1081 aaaatcggct cgtcgtattc gaattgccag agttagagac gtttgcgggc gtagaatttg
     1141 ccgaagtatt tccagccggc gtcgtgccgg aagtcgtgct cgcatcggcg gtcgaagcga
     1201 atttcataaa cgcgcccgtg tcgcccgaag gcgccgggaa tttgaaattg gtcgagccgg
     1261 ttccgttcga gtcgattttc tgcccgtcaa tcgtcacttc aatcagcgcg tcacccgctt
     1321 tctgcaagcg aaactcgtaa ttgaacgacg gcgatttgct cttgccgaaa agggcttcgc
     1381 gcaggcgaaa agcgttgttc agataaacga caaactcgtc cgagaatttg acctcgctcg
     1441 tcgctttcgg tttccactgc ccgttcgatt cttcaaaata tttcgccagc cgctcgtcga
     1501 aaaatttcga gaaagtgccg tccgccggat ttagataagc ggtcaatttg gtaatgtcgg
     1561 cttcgcccgc gtcttcaaac gggtagcctt tttcgatttc tttggctttc gggaaaatct
     1621 gttccgccca cgccttatcg agttggttgg tcacgccgac gccgagcaga ttgcgcagat
     1681 tcgtcagcgg ttttttcaaa atattggcta attcctgacc ggcgggcgtg tcgttaaagc
     1741 tgcccgtcat actggaaatc gctttttccg cgtcgcgcag cttcggctcc agtttcaagt
     1801 ctttctgctg cgcgatgtct ttcgtgacct ggctgaactc gtcctgcccg agcgcgtgga
     1861 aattcgagta aaccgactgg atttgcaagc cgtatttgtt gatttgcgac gattccggtt
     1921 tttcgtccgt gccgacaaag gcgaacagcg ggcgaaaatc tctttcgacg ggcgaattgc
     1981 cgccggtttc aatttttttc ttgctctgga aaaagctcgt cagccaatcc agccagcccg
     2041 ccggtttcgg cttgcccgac agattcgtct gccgcattat ttcccgcatc agcgcggtca
     2101 tcggcgaatt ggcggaggcg aatttttcca gcgcgttgtc ggcttccgct tgattcgcgt
     2161 agctgcggac gttgacgctt ttcacgaatt tgcgccattc gtc
//
LOCUS       DASWNE010000059         3572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_249496, whole genome shotgun sequence.
ACCESSION   DASWNE010000059 DASWNE010000000
VERSION     DASWNE010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249496"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..1231)
                     /locus_tag="VGB68_01275"
     CDS             complement(152..1231)
                     /locus_tag="VGB68_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEY0047887.1"
                     /translation="MNNLGVREYQSKLFTEWNTVKRNERSANDWLNKVEKTFREKGTF
                     ANEDLTFAAFVEMKKKTHLKEAIITDGKRVSGLKGFDSFVVHYLNPLVDSFGKAKIRL
                     IKAHDIDVYRNERLQGKTYKGTTRTIATVNRELSLLRKIFNLAVRDGVINFAPKFQIS
                     MADEGARERILDYAEQARLIEAFEYRNKQGRQTVKHLKPLVIFLLDTAVRFGEAQKLQ
                     WSDVNFDTGIITLRASNTKTAKERLIPMLPRVKDELTRLHEARTNESVFNVRDVKNGW
                     KTLCKVAEIEGCRIHDLRHSAITNFVRSGMRTEIAMAISGHSELRTFRRYLNFKANDF
                     IKAFEDVEAYKIRRETPELVSEASN"
     gene            1880..2509
                     /locus_tag="VGB68_01280"
     CDS             1880..2509
                     /locus_tag="VGB68_01280"
                     /inference="COORDINATES: protein motif:HMM:NF019958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0047888.1"
                     /translation="MSRSATRTNGSTTAKAVVTDKREAILRAAIKVFARNGYFNSKVS
                     DIAREANIADGTVYLYFKSKDEILHSVFDRAMEEFISEGKREISEIEKADEKLRRIAG
                     LHLERLSADRDLAVVFQVELRGSTKFMEEFSAAGFAEYLDIIRQTIVEGQKQGVFRAD
                     LNPTVCAKILYGALDEMVTNWILSKKSYPLAPMADTVLEIFFGGMVNGK"
     gene            2512..>3572
                     /locus_tag="VGB68_01285"
     CDS             2512..>3572
                     /locus_tag="VGB68_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0047889.1"
                     /translation="MISQIQDIRRKVQRIALFADEPQTLAELFLLAGEKRPRENALNY
                     KSGGEWKAISSGEMISRIENIALGLYSLGIRRGDRAALLAANSPEWTLTDAGCQFSGV
                     IDVPVYTTLAANQVCYIVNDSGAKVFFLQDREAYARLGGIFSECRSIEKLIFFNAEGV
                     AAENAMSLAELENLGARLKAEQPELLKELTAQTRSEDVATLIYTSGTTGEPKGVMLTH
                     ANIISNVIDAGENFTFSDKDKPLSVLPLSHIFERSGMYLYIFNGMAVHYAESIEKVPE
                     NLREVQPTIFVGVPRIFEKVYAKAKGKSASESRLKEKIFDWAIEVGQEYARRAEHKQP
                     IPRLLAVKHAIADKLVFRK"
BASE COUNT          906 a          846 c          808 g         1012 t
ORIGIN      
        1 taattattta ataaataaaa tacttgcgcc gtcgcctact tttgtatatt tgggtcggcg
       61 gaaattctgg caaaagttga tacttgcttt tgatacaggg catctatttt gctgataaat
      121 gccctgtaag ctgttttccc gaatgaaaca attagttaga cgcttcggaa acaagttcgg
      181 gtgtttcccg cctgattttg tatgcttcga catcttcaaa ggctttgata aaatcatttg
      241 ctttgaaatt caaatagcgg cgaaaagtcc gaagttcgct atgtcctgaa attgccattg
      301 caatttcggt tcgcattcca gaccgaacaa aatttgtgat tgccgaatgt ctaaggtcgt
      361 gaatccggca accttcgatt tcagccactt tgcaaagtgt tttccaaccg tttttaacgt
      421 ctctgacatt aaagaccgat tcgttcgttc tcgcttcgtg taatcgtgtc aattcgtctt
      481 tcactcgtgg caacatcggt ataaggcgtt ccttcgctgt cttcgtgttc gatgccctga
      541 gcgtgatgat tccggtgtcg aaattcacgt ctgaccattg taatttttgc gcctcgccga
      601 atctcacagc cgtatcgagt agaaaaataa caagtggctt gagatgcttt actgtctgtc
      661 tgccttgttt gtttcgatac tcgaatgctt ctatcaatct ggcttgttca gcataatcta
      721 aaattcgctc ccgtgcgcct tcgtctgcca tcgaaatttg aaacttcggc gcaaaattga
      781 taacgccatc cctgacagcc aagttaaaga ttttccgcaa aagcgataat tcacgattta
      841 cggttgcaat cgttcgggtc gtgcctttat aggttttgcc ctgcaaccgc tcatttcgat
      901 aaacgtctat gtcgtgcgcc ttaataagcc gaattttcgc ttttccgaaa gaatctacca
      961 aagggttaag ataatgaacg acaaacgagt caaagccctt taagcctgaa actcgcttgc
     1021 catccgtaat aatggcttct ttcagatgcg tctttttttt catttcgaca aacgccgcaa
     1081 aggttaaatc ttcgttggcg aacgtgcctt tttccctgaa tgttttttcg actttattta
     1141 accagtcgtt tgcagaccgt tcgtttcgct ttaccgtgtt ccactcggta aagagtttgc
     1201 tttgatattc ccgaacgcct aaattattca cttcggaaaa ttccgcccgt atgcctttgt
     1261 ctccgtattt tctcaatttt ctgctcatta tttttgctcc gtttttaatt tattcaggtt
     1321 tttgtgtccc cgtctgttaa ttaaaaacac gcaaaagcct tgtatttcct gtatttcacg
     1381 aaaaaacctt gtccccgaaa ttgtccccga aattaaatag ttgacacttt tccggtaact
     1441 tcaatagaca ttggaaaacg tctgtaagtt attgtattga caagtattat ttaatctgta
     1501 ataaggtttg aagcaggtat gtaaattagg tcaaaaatgg ctattcttag acttgaaaac
     1561 taccgcccgt aagggcttgc aggttcgagt cctgttctct ccgccacttt aattttctga
     1621 tttaggattg gtcgcaagcc agtccttttt cttttcctga caaaaaaata cattgaattt
     1681 gcagattttt gttgatttgt tttccggttt tgttatacct ttagcaaaac tgaatgatga
     1741 ttcattcatc catctgattt tagatttcgg atttgcgatt ttagattgaa agcgggattc
     1801 ggctcgtgcc gaatgccgta aaattaaaaa atccataacc agcccgcaaa tccgaaatcc
     1861 agaatctaaa ataaaaatta tgtcacgttc cgccacccga acaaacggtt cgaccacggc
     1921 gaaagccgtc gtcacagaca agcgcgaagc catcctgcgc gcggcgataa aagttttcgc
     1981 ccgcaacggt tatttcaatt ccaaggtctc cgacatcgcg cgcgaagcca atatcgccga
     2041 cggcacggtt tatctttatt tcaaaagcaa ggacgagatt ctgcattcgg ttttcgaccg
     2101 cgcgatggag gaatttatct ccgagggcaa aagggaaatc tccgaaatcg aaaaagccga
     2161 cgaaaaactt cgccgcattg ccgggcttca tctggagcgt ctgagcgccg accgtgattt
     2221 ggcggttgtt ttccaggtcg agctgcgcgg ctcgaccaag tttatggaag aattttccgc
     2281 cgccggtttt gccgaatatc tggacatcat ccggcagacg attgtcgaag gacaaaaaca
     2341 aggcgttttt cgcgctgatt taaacccgac cgtttgcgcc aagattcttt acggcgctct
     2401 ggacgagatg gtgaccaact ggattctgtc gaaaaaatcc tatccgctcg cgccgatggc
     2461 ggacacggtt ctggaaatat ttttcggcgg aatggtaaac ggtaaatgac gatgattagc
     2521 caaatacaag acatcagacg caaggttcag cgcatcgcgc ttttcgccga cgagccgcag
     2581 actctggcgg aactatttct gctggctggc gaaaaacgcc cgcgcgaaaa cgctctgaat
     2641 tataaaagcg gcggcgaatg gaaagcgatt tcgtccggtg aaatgatttc gcgcatcgaa
     2701 aacatcgcgc tcggacttta ttcgctcggc atccggcgcg gcgaccgcgc ggcgctgctc
     2761 gcggcgaact cgcccgaatg gactctgacg gatgcgggct gtcaattttc cggcgtgatt
     2821 gacgtgcccg tctacacgac gctggcggca aaccaggttt gttacatcgt taacgattcg
     2881 ggcgcgaaag ttttcttttt gcaggacagg gaagcttacg cgcgcctcgg cggaattttt
     2941 tccgaatgcc gctcaatcga aaaattaatt ttttttaacg cggaaggcgt cgcggcggaa
     3001 aatgcgatgt cgctggcgga gctggaaaat ctcggcgccc ggctcaaagc cgaacaaccc
     3061 gaattattaa aagaattaac cgcgcaaacc aggtctgaag acgtcgccac gctgatttac
     3121 acttccggca cgacgggcga gccgaaaggc gtgatgctga cgcacgccaa tattatctcc
     3181 aacgtcatcg acgcgggcga aaattttact ttttccgaca aagacaagcc gctctccgtt
     3241 ttgccgctgt cgcatatttt cgagcgttcg ggaatgtatc tctacatttt caacggaatg
     3301 gcggtgcatt acgccgaatc aatcgaaaaa gtgccggaaa acttacgcga agtccaaccg
     3361 acgatttttg tcggcgtgcc gcgcattttt gaaaaggttt acgccaaagc gaaaggaaaa
     3421 tcggcgagcg aaagccgtct gaaagaaaag attttcgatt gggcgattga agtcgggcag
     3481 gaatacgcgc ggcgcgccga gcataaacag ccgattccgc gtcttttagc cgtcaaacac
     3541 gcgattgccg ataaattggt tttcagaaaa tt
//
LOCUS       DASWNE010000060         2656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_638678, whole genome shotgun sequence.
ACCESSION   DASWNE010000060 DASWNE010000000
VERSION     DASWNE010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638678"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="VGB68_01290"
     CDS             complement(<1..745)
                     /locus_tag="VGB68_01290"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0047890.1"
                     /translation="MNLSNLVGQTIDEKYKIERELGKGGMGAVYLATHIGTERPVAVK
                     VIVPQFMQKAEFVERFKREARAAGRLRHPNVVDVTDFGFDDTPQGRVAYLVMEYLDGC
                     TLGEILEEEHQLPLEWSLDILEQVSSAVQTAHEQGIIHRDLKPDNIWLEPNQRGGYTV
                     KVLDFGIAKLEENMRATAAGALPNPSPHQTHANPRNSTVRDLDQAGTIAENQAGTIAE
                     NGNATILSEGATAALTNAVGDEAGTLIQPL"
     gene            997..2238
                     /locus_tag="VGB68_01295"
     CDS             997..2238
                     /locus_tag="VGB68_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047891.1"
                     /translation="MKLKNTRLHILHQARDLSQDAKTGVSLHCHTEYSKEMLDFVPHY
                     AEKLPVISYFWKKERDRYLEREGKGIDFSSAYWSPPMTEQDVYGIEKTQMNNAGLEAI
                     VSISDHDGIDANLKLSRHADNAVAPISLEWTVPFEYGFFHVGVHNLPKDRAIEITKTL
                     LDYTFGENPTNERLHEMFSMLNELPEVLVILNHPIWDIEMVGKERHEALLKNFLKEHG
                     RWIHAFEINGFRSWSENKAVIEMAEALGIPLVTGGDRHGCKPNTVINLTNSKTFGEFA
                     EEVRVDKHTEIVLMPEYKQPLHSRQLQSFSEILKLYPEFPEGRQKWFDRVHFDTGDGH
                     GVRKLSVHWKRGGPSWLRWAIWTLGILGHERLRPAFRLAMKKHDRVPKDLSKAKFEIP
                     ELEEIAASLAPEKPRETGFAS"
     gene            2306..>2656
                     /locus_tag="VGB68_01300"
     CDS             2306..>2656
                     /locus_tag="VGB68_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047892.1"
                     /translation="MSFTIVKETPRVAFFPDSFLEVNGVAMTSKRLIGYARRNGYPFL
                     CIHAGEKTKTYRDASVHYLSLKRSLIAFPMDEGLKYDPLFQRHSNRVLRELIQFKPDV
                     LHITGLNDVSIIGAY"
BASE COUNT          617 a          703 c          679 g          657 t
ORIGIN      
        1 ccagaggctg aatcaaagtt cccgcttcgt cgccgaccgc gtttgtcagc gcggcggtcg
       61 ccccttcgga aagaatcgtc gcgtttccgt tttcggcaat cgttcccgct tgattctcgg
      121 caatcgttcc ggcttgatcc agatcgcgaa cggtgctgtt tcgcgggttc gcgtgcgttt
      181 gatgcggcga cggattgggc aaagcgcccg ccgccgtggc gcgcatattt tcttcgagct
      241 tggcgatgcc gaaatccagc actttgaccg tgtagccgcc gcgctggttc ggttccagcc
      301 agatattgtc gggctttaaa tcgcggtgaa taattccctg ctcgtgcgcc gtttgaacgg
      361 cggaggaaac ctgttccaga atatcgagcg accattcgag cggcagttga tgttcttctt
      421 cgagaatctc gccgagcgtg cagccgtcca gatattccat caccagatag gcgacgcgtc
      481 cctgcggcgt gtcgtcgaaa ccgaaatcgg tgacgtccac cacgttcgga tgcctgagtc
      541 gtccggcggc gcgcgcctcg cgcttgaaac gctcgacgaa ttcggctttc tgcataaact
      601 gcggcacgat gaccttgacc gcgacgggac gctccgtgcc gatatgcgtg gcgagataaa
      661 ccgcgcccat tccgcctttg ccgagctcgc gttcgatttt gtatttctcg tcaatcgttt
      721 gtccgaccag atttgacaaa ttcataagac ttttttggaa actctactcg catttcatta
      781 cgtttggcaa gcttatatag attcatttat cttaggcgcg cgcggctgat ttttttccga
      841 ttgtgtgcta gactttttat cgacgctttg gctctcccgc acaatgacta attaagacaa
      901 caattaaggg gaattaaaca tctgacgaat ttacatcaac gggtgaattt gttaatttta
      961 tacatttaac caggtgggct ttatacggag aaataaatga agttaaaaaa cacgcgctta
     1021 catatcttgc atcaggcacg agacctgagc caggacgcga aaaccggtgt ttctctccat
     1081 tgtcataccg aatattccaa ggaaatgctg gatttcgtcc cgcattatgc cgaaaagctt
     1141 cccgtcattt cttatttctg gaaaaaggaa cgcgacagat atcttgaacg cgagggcaaa
     1201 ggcatcgatt tttcgagcgc ctactggtcg ccgccgatga ccgagcagga tgtttacggc
     1261 atcgaaaaaa cgcagatgaa caacgccggt ctggaagcta tcgtttcgat ttccgaccac
     1321 gacggcatcg atgccaattt gaaattgagc cggcacgccg acaacgccgt cgcgccgatt
     1381 tcgctggaat ggactgtgcc gtttgaatac ggatttttcc acgtcggcgt gcataatctg
     1441 ccgaaagacc gcgctattga aatcactaaa acgctgctcg attacacttt cggcgaaaat
     1501 ccgaccaacg agcgcctgca cgaaatgttc tcgatgctca acgagctgcc ggaagttctg
     1561 gtcattctga atcacccgat ttgggacatc gaaatggtcg gcaaagagcg tcacgaagcg
     1621 ctgttgaaaa actttttgaa agagcacggg cgctggattc acgcttttga aatcaacggt
     1681 ttccgcagct ggtcggaaaa caaagccgtc atcgaaatgg cggaagccct gggaatcccg
     1741 ctcgtcacgg gcggcgaccg tcacggctgc aagccgaaca cggttatcaa tctgaccaac
     1801 tcgaagactt tcggcgagtt tgccgaggaa gttcgcgtcg ataaacacac ggaaatcgtt
     1861 ttgatgccgg aatataaaca gccgcttcat tcgcgccagc tgcaatcgtt ttccgaaatt
     1921 ttaaagcttt atccggaatt tcccgaaggt cgccagaaat ggtttgaccg cgtgcatttc
     1981 gacaccggcg acggtcacgg agttcgcaag ctttccgttc actggaaacg cggcggtcct
     2041 tcgtggctgc gctgggcaat ctggacgctc ggcattctcg gtcacgaaag actgcgtccg
     2101 gcgtttcgtc tggcgatgaa aaagcacgac cgcgtgccga aggatttgtc gaaagccaaa
     2161 ttcgaaattc ccgagcttga ggaaatcgcc gcgagtctgg cgccggaaaa accgcgcgag
     2221 accggttttg cttcttaaag attttgttat caaatctaat ttgattttgt aatatcgatc
     2281 gccgccgcaa ggcggcattg catttttgag ttttactatc gtgaaagaaa ctccgcgcgt
     2341 cgcttttttt ccggattcgt ttctggaagt caacggcgtg gcgatgacca gcaagagact
     2401 tatcggatac gcaagacgaa acggctatcc ttttttgtgc atccacgcgg gcgaaaaaac
     2461 caaaacctac cgtgacgcaa gcgtccatta tctctcgctc aaacgctcgc tcatcgcttt
     2521 tccgatggac gaaggcttga aatacgaccc gctttttcag cgccattcaa accgcgtcct
     2581 gcgcgagctg attcaattca agcccgacgt tctgcacatc accgggttaa acgatgtgag
     2641 cattatcggc gcgtat
//
LOCUS       DASWNE010000061         7301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_567718, whole genome shotgun sequence.
ACCESSION   DASWNE010000061 DASWNE010000000
VERSION     DASWNE010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7301
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567718"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VGB68_01305"
     CDS             complement(<1..185)
                     /locus_tag="VGB68_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047893.1"
                     /translation="MDNLVFSNMMHRPARTLVSVMGIAIGVLLIVFTIGLANGTMRER
                     ATREANVGAEIMFRASGT"
     gene            complement(280..1914)
                     /locus_tag="VGB68_01310"
     CDS             complement(280..1914)
                     /locus_tag="VGB68_01310"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY0047894.1"
                     /translation="MASGIIIHVTVGKEKRTEFFSEERIRLGSDESSDLQIHTGKTAG
                     SIWLELERDQGVYRIIKFDESIGLLHNGKPMRRFLVIDDGDTIDVGSTGVSFSFFSLA
                     SKSALITTNRETHVAQFIEEAALDAAASPKRDDAKAFLREFVRELSREITWTTKIIVL
                     LLAISFIGVVLYLGNAVNQELRVSREQSQQQGEIIRKLEERLGQTSQQLGELDKTNKD
                     IIKTVSLAPNLRVEYGNGVCLIVGVYDLVDRKSGKTLRYLDPAAFQPDPYEPMEGEET
                     QQQQTAPPQTGLTTEGNGTPIEYDFIGTGFHVGGGYIVTNRHVVQPWAENDLVKQMMQ
                     QSNGKARIKRLVVYFPNMPQPIALKMQQFGSKEDLAVGTIDPNSVSPDIPVLPIDTAT
                     EAMAIGKTVVTMGYPSGPDRLLAMVDDDEARAINSRFGNSRQALLNYLAQSRKIVPLT
                     TQGAITDLDTRRIVHDAKTAEGGSGAPLFGQSGKVIGVNFGVFTENTAANMAVPIKFA
                     VDLLKKAGWKSPEEIQAQQNQTTQTASANTNSSATQ"
     gene            2148..2546
                     /locus_tag="VGB68_01315"
     CDS             2148..2546
                     /locus_tag="VGB68_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEY0047895.1"
                     /translation="MSERKFIDDREKLEKLAPVAAAPIKHHVFVCNGKSCAAVGGAEV
                     KAAFERELESRNLRYGKPSKGRNPNGSVVLTDCGSVGFCSIGTAVLVYPEGVWYAQVR
                     AEDVAEIVEKHLIGGKIVERLALVEMDKKV"
     gene            complement(2692..4203)
                     /locus_tag="VGB68_01320"
     CDS             complement(2692..4203)
                     /locus_tag="VGB68_01320"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEY0047896.1"
                     /translation="MARIFISYRRRDSAYVAGSLSEKLQQHFGEDSVFYDIDNIPLGV
                     DFREHIGNAVGQCDALLVIIGEEWVTAVDKNGGRRLDNPGDYVRIEIESALKRNIPII
                     PVLVEEAQMPAAADLPEAIQSIVYRNAAEIRAGSDFRQHIERLIRGLESLFAANEKTK
                     PEERKKEPVRKPRATAGKTEKPRRKESKSSVLPQPKAIKPQAEIASKEFVAKFVIDGV
                     LDDFTHEYLFRHGAIPENKSQNAIQAYAQDVRHEDILLLYDNTFFRSAKDGLLITADA
                     VYWHNYMEDVGTARFSDITNVTYEDGVFSSEIYIDGRKIKLQTGDRTHSTRILASLIL
                     SLATAIRDYELGKGWKKAQMQLIVKSDDGACQLSFPLGWSKQPDLNSPAATLKVGNPD
                     EELYVIIIPELKSDFDAGTTLDAYADLVRGNLRKLLAEPDFPEPVSTIVGGFPARQFE
                     AGGIYSGIKVKYLYAVVGTLNNYYQIMSWTPAENYRKNKAELLGVIKTFKAIS"
     gene            4260..4481
                     /locus_tag="VGB68_01325"
     CDS             4260..4481
                     /locus_tag="VGB68_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047897.1"
                     /translation="MVCAQKPERFSATQFGQNGDKPTPAAPDGDGKSGIDVRRGRRWM
                     IKRSSDETANPGAIFGLATGASVQPAFVR"
     gene            complement(4478..4792)
                     /locus_tag="VGB68_01330"
     CDS             complement(4478..4792)
                     /locus_tag="VGB68_01330"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEY0047898.1"
                     /translation="MNCPVCNVELKMSDRQGVEIDYCPRCRGVWLDRGELDKIIERSE
                     IGIESTERDRYDEHNRQRDDSNRRSEEHNRYSEHGSNYPQQPYKKHKKRESFLGNLFD
                     FD"
     gene            complement(5077..6927)
                     /locus_tag="VGB68_01335"
     CDS             complement(5077..6927)
                     /locus_tag="VGB68_01335"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY0047899.1"
                     /translation="MRFCKRFSLVVLLALLVAVQVSADTPIWSYKGAKWYAMMDTGNL
                     IVANDTSLAMVDGANGSVIWKRDDLKGIQETEYNEIAGTPVLLVADNFGSLAKKTRIY
                     ALDQLTGKTLWETEKVYGYTVQVSPVRNRDMVVVLTTKSNAVTKDKPDITALKITTGE
                     VLWQTEYPEKVDLYVKEKFRRSGSGAAFAKAMFGNPFAGFSDQPRYDLSGENPPIFDG
                     DSMYMTYAGLHRYDLKTGNLIWKTVYDVTDGSLKRTNGQAIIGGDTIYTSAKNIIRAI
                     DRNSGAIRWQTKDFGEGGIAEMQLHGDVLFGRMGGQFFSGKKREYQKKTPIGVVALSK
                     ASGNVNWIYTGAKNSITNMVVLPDENVMLVGDEKNIIGLDLASAGKVKESYKIPLKFK
                     FKLGAGAVAGKIAKIGFGGIGGAFSKGPDTTDEPVALIRQENGTVVARGRQHMLAFDP
                     KSRQIVWSTKYAAPGVEGWQQIVMTAITIASAAVSQGFESSYARRGDYNSAFRENDNF
                     INLMTSYQNFMETRFMNTKRVGNVVYVLTDVKSGKDKGAGLVGVNLMTGQGASQIMFK
                     DKTPDYEVDEASGRLFNLNKGELVAFSINEKAEMEQNKDEDDDKEKNKDK"
BASE COUNT         1647 a         1940 c         1865 g         1849 t
ORIGIN      
        1 gtccccgacg cgcgaaacat aatttccgcg ccgacgtttg cctcgcgcgt cgcccgctcg
       61 cgcatcgtgc cgtttgccag cccgatagtg aaaactatga gcaaaacgcc gatggcaatg
      121 cccatgacgc tgaccagagt gcgcgccgga cgatgcatca tatttgaaaa aacgagatta
      181 tccataaatt tcaattacga attacgaatt acgaattaca aacgtttgaa ttaagtcaac
      241 cattcgtaat tcgtaattcg taattcgtaa ctcacgattc tactgcgttg ctgagctgtt
      301 tgtatttgcg cttgccgtct gcgtcgtctg gttttgctgc gcttgaattt cttcgggcga
      361 tttccagccg gcttttttca gcaaatcgac ggcgaatttt atcggcacag ccatatttgc
      421 cgccgtattc tcggtgaaaa cgccgaaatt gacgccgatg accttgccgc tctgaccgaa
      481 cagcggcgcg ccggagccgc cttccgccgt tttggcgtcg tggacgatgc ggcgcgtgtc
      541 taaatccgta atcgcgccct gcgtcgtcag cggcacgatt ttccgcgatt gcgccagata
      601 attcaaaagc gcctgtctgg aatttccgaa acgcgaattt atcgcgcgcg cttcatcgtc
      661 gtcgaccatc gccagcagcc ggtcgggacc gctcggataa cccatcgtga cgacggtttt
      721 gccaatcgcc atcgcttcgg tcgccgtatc aatcggcaga acgggaatgt cgggcgagac
      781 cgagttcggg tcaatcgtgc cgacggctaa atcttccttc gagccgaact gctgcatttt
      841 gagcgcaatc ggctgcggca tattcggaaa ataaacgact aatcgcttga tgcgcgcctt
      901 tccgtttgac tgctgcatca tctgcttgac caaatcgttt tccgcccacg gctgcacgac
      961 gtgtcgattg gtgacgatat aaccgccgcc gacgtgaaaa cccgtgccga taaaatcata
     1021 ttcgatgggc gtcccgttgc cttcggtcgt caatccggtc tgcggcggcg cggtttgctg
     1081 ctgctgggtt tcctcgcctt ccatcggttc gtacgggtcg ggttgaaaag ccgccgggtc
     1141 gagatagcga agcgttttgc cgcttttgcg gtcgacgagg tcgtaaacgc cgacaatcag
     1201 acagacgccg ttgccgtatt cgacgcgcag gtttggcgcg agcgaaacgg ttttgataat
     1261 gtccttattg gttttgtcca gctcgccgag ctgctggctg gtctgtccga gtctttcttc
     1321 gagcttgcgg atgatttcgc cctgctgctg tgactgctcg cggctgacgc gaagctcctg
     1381 gttgacggcg ttgccgagat acaaaactac gccgataaag ctgatcgcca gcagcagaac
     1441 gataattttg gtcgtccatg tgatttcgcg cgaaagctcg cggacgaatt cgcgcaggaa
     1501 agctttggcg tcgtcgcgct tgggcgaagc cgccgcgtcc agcgccgctt cttcgataaa
     1561 ctgcgcgacg tgcgtttcgc ggttggtcgt aatcagcgcc gattttgagg cgagcgaaaa
     1621 gaaggaaaac gaaacgccgg tcgagccgac gtcaatcgta tcgccgtcgt ctatgacgag
     1681 aaaacggcgc atcggcttgc cgttatgcag taatccgatt gattcgtcaa acttgatgat
     1741 gcggtagacg ccttggtcgc gctccagttc gagccagatg ctgccggcgg ttttgccggt
     1801 gtgaatctgc aaatcgctcg attcgtccga gccaagacga attcgttcct cggaaaaaaa
     1861 ttccgtgcgc ttttccttac cgaccgtgac gtgaatgatg ataccgcttg ccattcagta
     1921 gattgagtaa cactcctaaa cgcttatagt aacacgagcc aaagaattta gatgcgggaa
     1981 atgaggcatt ttttaaaaat ctttatcaaa gtggtaagcg gtaaacggga agtgaaagaa
     2041 tttatcattt gccgtttatc acccggcgct gaaaacacaa aatgcaaaac ccggaattag
     2101 cagcttaccg gttatcgttt agcctttttt cgcgttaaaa ttgaaaaatg agcgaaagaa
     2161 aatttatcga cgaccgcgaa aagctggaaa agctcgcgcc ggtcgccgcc gcgccaatca
     2221 aacatcacgt ttttgtctgc aacggaaaat cctgcgcggc ggtcggcggc gctgaagtca
     2281 aagcggcttt tgaaagagag ctggaatcga gaaatctgcg ctacggcaag ccgtcgaaag
     2341 gcagaaaccc gaacgggtcg gtagtgctga ccgattgcgg gtcggtcggt ttctgttcaa
     2401 tcggcacggc ggttctggtt tatcccgaag gcgtctggta cgcgcaggtg cgcgccgaag
     2461 acgttgcgga aatcgtcgag aaacacttaa tcggcggaaa aatcgtcgag agactggcat
     2521 tagttgaaat ggataagaag gtttagcgct caggtcaggc gcgcgccgga gaaacggcac
     2581 ggaagccgca agttaccgcg cagcgggaag cgctaaagca atcgacggtc ttcacaacga
     2641 tttttcacgt tctgatttct ttcggcgcgg tgcggcgatt gcgtcatcgg ctcagcttat
     2701 tgccttaaat gtcttgatga cgccaagcag ttccgcttta tttttcctgt aattctcggc
     2761 gggcgtccag ctcataatct ggtaatagtt gttcagagtt cccacgaccg cgtaaagata
     2821 ttttaccttt atgcccgaat agattccgcc cgcctcgaat tgtcttgccg gaaagccgcc
     2881 gacgattgtc gaaaccggct cgggaaaatc cggctccgcc agcaatttac gcaagttgcc
     2941 gcgcaccaaa tcggcataag cgtcgagagt cgtcccggcg tcgaaatcgg atttgagctc
     3001 ggggataata attacataaa gctcttcatc cgggtttccg acctttagcg tcgccgccgg
     3061 cgaattcaaa tcgggctgct tgctccaacc gagcggaaag gaaagctggc aagcgccgtc
     3121 gtcgctcttc acgatgagct gcatctgagc ctttttccag ccttttccca gctcgtaatc
     3181 acgaatggcg gtcgccaggc tgagtattaa gctggcgagt attctcgtcg aatgggtgcg
     3241 gtcgcccgtt tgcagcttga ttttgcgccc gtcgatatat atttcgctgg aaaaaactcc
     3301 gtcttcgtaa gttacgtttg taatgtccga aaaacgcgcc gttccgacgt cttccatgta
     3361 attgtgccag tagacggcgt ccgcggttat aagcagtccg tccttcgcgc tcctgaagaa
     3421 agtgttgtcg taaagcagca aaatatcttc gtgccggaca tcctgagcgt aagcctgaat
     3481 cgcgttctgc gacttatttt ccggaatggc tccgtgccga aataaatatt cgtgggtgaa
     3541 atcgtctaaa acgccgtcaa ttacaaattt tgcgacgaat tccttgctgg ctatctccgc
     3601 ctgcggtttt atcgctttcg gctgcggcaa aacggaagat ttcgactctt tacgccgagg
     3661 tttttccgtc ttcccggcgg tcgccctcgg ttttcggacg ggttcctttt tcctctcttc
     3721 cggttttgtt ttttcattgg cggcgaaaag actttcgagc ccgcgaatca gccgttcgat
     3781 atgctgccgg aaatccgagc cggcgcgaat ctcggcggca tttcgataga cgatgctttg
     3841 aatggcttcg ggcaaatcag ccgccgccgg catctgcgct tcctcgacca gcacgggaat
     3901 gatgggaata ttgcgcttga gcgcggattc aatctcaatc cgcacataat cgcccggatt
     3961 gtccagccgt ctgccgccgt ttttatcgac ggcagtgacc cattcttcgc cgatgattac
     4021 gagcaaagcg tcgcattgcc cgacggcgtt tccgatatgt tcgcggaaat cgacgccgag
     4081 cggaatgttg tcgatatcgt aaaaaaccga atcttctccg aagtgctgct gcagcttttc
     4141 gctcagagaa cccgcaacat atgcgctatc gcggcgacgg tatgatataa agattcgtgc
     4201 cataaccctg agttaaaatt tataaagcaa ttcgctcgat tatacaagac tgacggcatt
     4261 tggtatgtgc tcagaagccg gaacggtttt ccgcgactca attcggacaa aacggcgata
     4321 aaccaacgcc cgccgcgccg gacggcgacg gcaaaagcgg catcgacgtc cggcgcggca
     4381 ggcgctggat gataaaaaga agctcggacg aaacggcaaa tcccggagca atttttgggc
     4441 tggcaaccgg cgcgtccgtt caaccggcgt ttgttcgtta atcgaaatca aacaaattgc
     4501 ccagaaaaga ctcgcgcttt ttgtgctttt tatacggctg ctgcgggtaa ttagagccgt
     4561 gttccgaata acgattatgc tcttcagagc ggcggttgct gtcgtcgcgc tggcgattat
     4621 gctcgtcata cctgtcgcgt tcggttgact cgattccgat ttcggagcgc tcgataattt
     4681 tatcgagctc gccgcggtcg agccagacgc cgcggcagcg cgggcaataa tctatctcga
     4741 ccccctggcg gtcggacatt ttcaattcga cattacaaac cggacagttc atagaataat
     4801 tcacctctta tagaaagata ttaatagaga gaaatttgaa aggcaacaaa aggcagaggc
     4861 gggcggcaag cccgcctttc ggcgatattg aaaaaacgtt ttccgaaaaa gcccggcgca
     4921 gccggatttt cgataggcga agtattattc cggtcggagc gaaattgtcg atataaattt
     4981 ccggtgaaat caatgtgaag aaaaaagccc gctcgtttca agcaggcttt tagtctttga
     5041 ttatcgtctt tcgcgcgttt atttgtcttc gcgcgtttat ttgtctttgt tcttttcctt
     5101 atcgtcgtcc tcgtccttgt tctgttccat ctcggctttt tcattgatgg aaaacgcgac
     5161 cagctcgcct ttgttcaaat tgaaaagcct gcccgaagct tcgtcgactt cgtaatccgg
     5221 cgttttgtcc ttgaacataa tctggctggc gccctgtccg gtcatcaggt tgacgccgac
     5281 cagtccggcg cccttgtcct tgccgctttt cacgtcggtc aaaacgtaga cgacgtttcc
     5341 gacgcgcttg gtattcataa aacgcgtttc cataaagttt tgatagctcg tcatcagatt
     5401 gatgaaatta tcgttttcgc gaaaggcgga attgtaatcg ccgcgccggg cgtaagacga
     5461 ttcaaaaccc tgcgagaccg ccgccgaagc aatcgtaatg gcggtcatca cgatttgctg
     5521 ccagccttcg acgcccggcg cggcgtattt cgtcgaccag acgatttgtc tggatttcgg
     5581 gtcgaaagcg agcatatgct ggcgaccgcg cgccaccacg gttccgtttt cctgacgtat
     5641 cagcgcgacg ggctcgtccg tcgtgtcggg accttttgaa aacgcgccgc cgattccgcc
     5701 gaagccgatt ttggcgattt ttccggcaac cgcgccggcg ccgagcttga acttaaattt
     5761 cagtgggatt ttataagatt ccttaacctt gcccgcgctc gccaaatcca gaccgataat
     5821 atttttctcg tcgccgacga gcatcacgtt ttcatcgggc aagacgacca tattcgtgat
     5881 ggaatttttc gcgcccgtgt aaatccagtt gacgtttccg ctggctttac tgagcgcgac
     5941 gacgccgatc ggcgtttttt tctggtattc gcgttttttg cccgaaaaaa attgcccgcc
     6001 cattctgccg aaaaggacgt cgccgtgaag ctgcatttcg gcgatgccgc cttcgccgaa
     6061 atcttttgtc tgccaccgaa tcgcgccgga attccggtca atcgcgcgaa taatgttttt
     6121 cgccgaagtg taaattgtgt cgccgccgat aatcgcctgc ccgttcgtcc gtttcagaga
     6181 gccgtccgtc acgtcataaa ccgttttcca gattaaattt ccggttttca aatcgtagcg
     6241 atgcaatccg gcataagtca tatacatcga atcgccgtcg aaaatcggcg gattttcgcc
     6301 gctcaaatca tatcgcggct ggtcagaaaa acccgcaaaa ggattgccga acatcgcttt
     6361 cgcaaacgcc gcgcccgaac ccgagcggcg aaatttttcc ttgacgtata aatcgacttt
     6421 ttcgggatat tccgtctgcc agagcacttc gcccgtcgtg attttcagcg ccgtaatgtc
     6481 cggtttgtct ttcgtcacgg cgttgctttt cgtcgtcaga acgacgacca tatcgcgatt
     6541 tctcacgggc gaaacctgca ccgtgtagcc gtaaaccttc tcggtttccc aaagagtttt
     6601 gccggttaac tggtcgagcg cgtaaatcct ggtttttttg gcgagcgagc cgaaattatc
     6661 ggcaaccagc aaaaccggcg ttcccgcaat ctcgttatat tcggtttcct ggatgccttt
     6721 caaatcgtcg cgcttccaga taacgcttcc gtttgcgccg tcaaccatcg ccagactcgt
     6781 gtcattggcg acaatcaaat ttcccgtatc catcatcgca taccacttcg cgcctttgta
     6841 cgaccaaatc ggcgtgtcgg cggaaacctg cacggcgaca agcagcgcca gcaaaacaac
     6901 cagcgaaaac cttttacaga atctcatttt cttaaactcc ttcgattatt atattaaaaa
     6961 ataaaacgcg aatgtttccg tcagtaatcg cagtaaaagg cgggaaaaaa ggtcagcatc
     7021 aatcgctgct tataaataat tgaattattt cgtttatttg agctttctta ctacagatac
     7081 gagctcgtaa tcagggcggg cgaattcgtg ttccggcttc tccggctcga atacgggctg
     7141 acggctcccg agccggaatt aacgagatat ttgttcgttt attgctttaa tatttgaaaa
     7201 gagctaatat ctgcaaaacc gacatacctg ccgccccaca ggatatacaa actcaggaga
     7261 aaaaccaatg tattcattat ccgaaacagc gccgagagcc a
//
LOCUS       DASWNE010000062         2461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_532007, whole genome shotgun sequence.
ACCESSION   DASWNE010000062 DASWNE010000000
VERSION     DASWNE010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532007"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="VGB68_01340"
     CDS             <1..833
                     /locus_tag="VGB68_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0047900.1"
                     /translation="VVFSSIFLLLLLIGAYAYFIEPRRLVINENAISVPHWSANLNGF
                     RVVAISDIHGGSNYVTEERLRELVTKANAQNPDLIVLLGDYVSERFRDHKNLRMPVET
                     IAENLKGFRARLGVYAVIGNHDWWYDEQKVRAEFEKAGIKFLENEAVSFDVNGEKIWL
                     LGIEDFWKERRVEVSDAMRQIEPKKNIIAITHNPDSLLHSPAEISLMLAGHSHGGQVR
                     FPFYGAAAFVNDARFMKGEATVDGKHVFVTTGVGCSGPPVRFGVTPEIAVLQIDSKSE
                     "
     gene            830..1732
                     /locus_tag="VGB68_01345"
     CDS             830..1732
                     /locus_tag="VGB68_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0047901.1"
                     /translation="MKIGKNKIISGLILIIIAAAACLLYAYFIEPNRLVVNEYDIKIK
                     NWNPALNNLKIVAVSDIHGGSNYIDEAKIRRVVELANAQNADLIVILGDFVSQRHDDK
                     PINQRDLKMPMETIGENLKGLRARLGVFAVLGNHDEWYNDRIVEIELEKAGITVLQNE
                     AVALEKDGHKFRVLGLLDHMRIVDWAIFAKNAKENLAKIEPAGDVIVLEHSPDILPVI
                     TGDSPLSPDVRLILAGHTHGGQVCLPLVGCPVIPSSYGQKYAYGHVRENNVDMFITTG
                     VGTSILPLRFNVAPEIAVLKIYAQ"
     gene            complement(1951..>2461)
                     /locus_tag="VGB68_01350"
     CDS             complement(1951..>2461)
                     /locus_tag="VGB68_01350"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0047902.1"
                     /translation="GLPSDPIFLVLLFLETQIVAFIGALFWERVSRKIGAKNTILVSL
                     AWWAGIVIFAYGFLNDKSQAWYLGAGIGFVIGGTQSLSRSLYSQLIPKGREASFFSFY
                     EISEKGTSWMGLLIFSVVVASTGSYRHAILALIFFFVVGGLLLAFNPINKAIREAGQH
                     TPEEAAAEQ"
BASE COUNT          635 a          632 c          647 g          547 t
ORIGIN      
        1 cggtcgtatt ctcgtcgatt tttttgctgc tgcttttaat cggcgcttat gcttatttta
       61 tcgagccgcg acgcctggtt atcaacgaaa atgcgatttc cgtcccgcac tggagcgcga
      121 atctgaacgg ctttcgagtc gtcgccatct ccgacattca cggcggctcg aattacgtca
      181 ccgaagaaag actgcgcgag ctggtgacga aagccaacgc gcaaaatccc gatttgattg
      241 tgcttttagg cgattacgtc tcggaaagat tccgcgacca taaaaatctg cggatgccgg
      301 tcgaaacgat tgccgaaaat ttaaaaggct ttcgggcgcg gctgggcgtc tatgccgtta
      361 tcggcaatca cgactggtgg tacgacgagc agaaagtccg cgccgaattt gagaaagccg
      421 ggataaagtt tctggaaaac gaagcggttt cgtttgacgt caacggcgaa aaaatctggc
      481 ttctcggcat cgaagatttc tggaaggagc gccgcgtcga agtttccgac gcgatgcggc
      541 aaatcgagcc gaaaaaaaat attatcgcca tcacgcacaa tcccgattcg cttttgcatt
      601 cgcccgcgga aatcagcctg atgctcgccg gacattcgca cggcgggcag gtgcgctttc
      661 ctttttacgg cgcggcggcg tttgtcaacg acgcgcgatt tatgaaaggc gaagcgacgg
      721 ttgacggaaa acacgtcttt gtcacgacgg gcgtcggctg ttcgggaccg ccggttcgtt
      781 tcggcgtgac gccggaaatc gccgttttgc aaatcgattc aaaaagcgaa tgaaaatcgg
      841 aaaaaacaaa atcatttcgg gcttgatttt aattattatc gcggcggcag cctgtctgct
      901 ttacgcttat ttcatcgagc cgaatcgcct cgtcgtcaac gaatacgaca taaaaatcaa
      961 aaactggaat ccggctttga acaatttgaa aatcgtcgcc gtttcggata ttcacggcgg
     1021 ctcgaattat atcgacgaag cgaaaatccg ccgcgtcgtc gaattagcca atgcgcaaaa
     1081 cgccgattta atcgtgattt tgggcgattt cgtttcgcaa aggcacgatg ataaaccgat
     1141 taaccagcga gatttgaaaa tgcccatgga aacaatcggc gaaaatctca aaggcttgcg
     1201 cgcgaggctc ggcgttttcg ccgtgctggg caatcacgac gaatggtata acgaccggat
     1261 cgtcgaaatc gagctggaaa aagcgggcat cacggttttg cagaacgaag ccgtcgcgct
     1321 ggaaaaagac ggtcacaaat ttcgcgttct cggtttgctc gaccatatgc gcatcgtcga
     1381 ttgggcgata tttgccaaaa acgcgaagga gaatttagcg aaaatcgagc ctgccggcga
     1441 cgtgatagtg ctggaacaca gccccgacat tctgccggtc atcacgggcg attcgccgct
     1501 ctcgcccgat gtccggctga ttctcgccgg ccacacgcac ggcggtcagg tctgcctgcc
     1561 gctggtcggc tgtccggtga ttccctccag ctatggtcag aaatacgctt acggtcacgt
     1621 gcgcgaaaat aatgtcgata tgtttataac gacgggcgtc gggacaagca ttctgcctct
     1681 gcgctttaac gtcgcgccgg aaattgcggt tttaaagatt tacgcgcaat gagaatcagc
     1741 cgcgaataag cgcgaaaaaa aaacgaaata ataatcaaga aatttagccg ccgccgaaac
     1801 acggaaaaag aagaaattaa ccgggaataa gcaggcttcg cggctcgata gacaaaggca
     1861 tttaaaataa aaatccggct tattctgctt gtccggcttt gccttgtgaa tttttctttc
     1921 gcgttttttc gcgttctgcg gcggctgatt tcattgttcc gccgccgctt cttcgggcgt
     1981 gtgctgtccg gcttcgcgaa tcgctttatt tatcgggttg aaggcgagca gcaatccgcc
     2041 gacgacgaaa aagaaaatca acgccagaat cgcgtgccgg tacgagccgg tcgaggcgac
     2101 cacgaccgaa aaaatcagca agcccatcca cgacgtgcct ttttcggaaa tctcgtaaaa
     2161 gctgaaaaag gaagcttcgc gccctttcgg aatcaactgc gaataaagcg accgcgaaag
     2221 cgattgcgtt ccgccgatga caaaaccgat gcccgcgccg agataccacg cctggctttt
     2281 atcgttcaga aaaccgtagg cgaaaatgac gattcccgcc caccacgcca gcgagaccaa
     2341 aatagtgttt ttcgcgccga ttttgcgcga gacgcgttcc cagaaaagcg cgccgataaa
     2401 agcgacaatc tgcgtttcca gaaataacag gaccagaaaa atcgggtcgg acggcaagcc
     2461 c
//
LOCUS       DASWNE010000063         4500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_638733, whole genome shotgun sequence.
ACCESSION   DASWNE010000063 DASWNE010000000
VERSION     DASWNE010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638733"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VGB68_01355"
     CDS             <1..207
                     /locus_tag="VGB68_01355"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEY0047903.1"
                     /translation="NPNICNGKPTIRNKRITVQTILEFLSNGDSVEEILAQYPTLTEE
                     DVYACLKFATSLMEHNYIVEPVNA"
     gene            204..545
                     /locus_tag="VGB68_01360"
     CDS             204..545
                     /locus_tag="VGB68_01360"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HEY0047904.1"
                     /translation="MNKYLIDANLPRKIKVWQTEDFQFVSEINDECTDSEIWDYAKQN
                     NLTIVTKDADFSHRIIVIQPPPKIIHIKIGNMKLRDFESAIEKVWKTSEELSGTHKLV
                     NVFIDRIEAVN"
     gene            complement(546..2408)
                     /locus_tag="VGB68_01365"
     CDS             complement(546..2408)
                     /locus_tag="VGB68_01365"
                     /inference="COORDINATES: protein motif:HMM:NF019758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047905.1"
                     /translation="MRFKLPEIPPPQLIASLRSYNLLPAIIFMPTRRRCDESAMEVAL
                     KKQNSSPERSEARRRIFQEYAEQNPEIGRHKHKNLVIRAGVASHHAGHIPAWKLLIEK
                     MMSAGLLDAIFATTTVAAGVDFPARTVVISNADTRGNDGWRSLQASELQQMTGRAGRR
                     GKDNVGFVILAPSNFQNPPRIATLLKSPPDALASQFRATYSSLLNLLDAYGSFPQVRE
                     IAEKSFAFRETAHQVLRLERKREKRQANIAEKIKASGFDLSIDAVRGFERLTATRNRL
                     QEKLPMRRAEIRHKWLEENVASGRVVSQGRSGKRFFLVLNVYGEKVTAMKDDGGGTTF
                     ALSRVNRVYAKKYPLNDKSVETAFFEIYEGKNPPVDEPRIVLTQNENDEAVEIINQLI
                     ERLLPGDLSDDRKRLAHQFLWESWTDAEFIEKTGRDIDVLRDEIWLPFEHRARVLHHF
                     GYLDFPAQKVTASGKWLADLRVDRPLLVGEALRQGLFEKLETKHVAGLMAALAGDSDR
                     NYGELHLSDKILDTLTEFEDIVFAVSGVEWKFGVEPAPEMNFSAAATAEHWADGASWE
                     NLVRETKAEEGDLVRLLSRTGEALLQIANLKKSNPEAAEIARETAEIILREPIR"
     gene            complement(2601..>4500)
                     /locus_tag="VGB68_01370"
     CDS             complement(2601..>4500)
                     /locus_tag="VGB68_01370"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEY0047906.1"
                     /translation="GGGSITKVEFYRRQGAQTVLLATDTTAPYEHQLTGVAAGNHGFF
                     ARAFDSGGAVKDSQYVEVTVNAPVTTVRLQGDIYNPDGFPMQGIRVNLTGTTATGTPF
                     NQTSISLSTGAYGFGNVPAGGNYTITPQVVGTLTFLPASFSVTNATQDNLDIDFLSSA
                     PNQSPSVTINSPANGAVFTMPAAIPVNVTATDPDGTIVHLTVSAVGNSQSTTIGQSNN
                     GTFNAPWQPNRPGEYRLWAQARDNGGFITSVFIDITVNPPAPVGISGRIVDRNSIGIE
                     GVTLELRNYPEDEENPQPPVATVTTNAGGNYTISNVTTFGNYILRASKMDYTFSPRQR
                     IYLNPATSQTGDFTGTIQVQPSEFDGDGRSDLAVWRPETGDWHVMRSSDNTYTGTRFG
                     GGSFGDIAVPGNYDGDRKTDLAVYRPSDGTWYIAQSSNGQVRAVQFGIATDKPAAGDF
                     DGDGKTDFAVFRPSNGYWYILRSTDNQFIAIQWGTDGDTPLAGDFDGDGIADITVFRP
                     SDGTWYVRRSTTGNLQGFRFGQNGDVPLVGDFDGDKQADFTVFRPSDGVWYVWKSSDN
                     SLKAFKWGTSGDKPVPGDFDRDGKTDFAVFRPTDGNWYIFRSTTNSYTVDKFGQNGDV
                     PIPAAYVQ"
BASE COUNT          997 a         1226 c         1220 g         1057 t
ORIGIN      
        1 aatccgaata tctgcaacgg caaaccgact attcggaata aaagaatcac ggtgcagaca
       61 attcttgaat tcctcagcaa cggcgattcg gtcgaagaaa ttctcgctca atatccgact
      121 ttaaccgaag aagatgttta cgcctgcctg aaatttgcga caagtttaat ggaacataat
      181 tacatcgttg agcctgttaa tgcatgaata aatatctcat tgatgccaat ctgccccgaa
      241 aaattaaggt ttggcagaca gaagacttcc aatttgtcag cgagattaac gatgaatgca
      301 cagacagcga aatttgggat tacgcaaaac agaataatct aactattgtc actaaagatg
      361 ctgatttctc gcatcgaatt atagttatcc agccaccgcc taaaatcata cacattaaaa
      421 ttggaaatat gaaattgcga gattttgagt cggcaattga gaaagtgtgg aaaacgtcgg
      481 aagaattatc cggaactcat aagctggtta atgtatttat agaccgcatt gaagccgtaa
      541 attaactaac gaatcggctc gcgcaggata atctccgccg tctcgcgcgc gatttccgcc
      601 gcttccgggt tggatttttt caggttcgcg atttgtaata atgcttcgcc cgtgcgcgac
      661 aaaaggcgca ccaaatcgcc ttcttcggct tttgtctcgc gcaccagatt ctcccagctc
      721 gcgccgtccg cccagtgttc ggcggtcgcc gccgccgaaa aattcatttc cggcgcaggc
      781 tcgacgccga atttccattc cacgccggaa accgcgaaaa cgatgtcctc aaattcggtc
      841 agcgtgtcca gaattttatc cgacaaatga agctcgccgt aatttctgtc cgaatcgccg
      901 gcgagcgccg ccatcagccc ggcgacgtgt ttcgtttcga gcttttcgaa aagcccttgc
      961 cgcagcgctt cgcccactaa aagcggtctg tcgacgcgca aatcagccag ccattttccg
     1021 cttgccgtga ctttttgcgc cggaaaatcc agatagccga aatgatgcag aacgcgcgcg
     1081 cgatgctcga acggaagcca gatttcgtcg cgtaagacgt cgatgtcgcg tccggttttt
     1141 tcaataaatt cggcgtccgt ccagctttcc cacaaaaatt gatgcgccag gcgtttgcgg
     1201 tcgtcgctca aatcgccggg caaaagccgc tcaatcaatt gattgataat ttccaccgcc
     1261 tcgtcgtttt cgttttgcgt cagcacgatg cgcggttcgt cgaccggcgg atttttgcct
     1321 tcgtaaatct cgaaaaacgc ggtttcgacc gatttatcgt taagcggata ttttttggcg
     1381 taaacgcggt tgacgcgcga cagcgcaaac gtcgttccgc cgccgtcgtc tttcatcgcc
     1441 gtcacttttt cgccgtaaac gttcagcacc agaaaaaaac gcttgccgct tcgaccctgc
     1501 gaaacgacgc gccccgaagc gacgttttct tccagccatt tatgacggat ttccgcgcgg
     1561 cgcatcggaa gtttttcctg caggcgattg cgcgttgcgg tcaggcgctc gaaaccgcgc
     1621 acggcatcga ttgataaatc gaaaccgctc gctttgattt tttccgcgat attcgcctgc
     1681 cgtttttcgc gctttctttc cagacgcaga acctgatgcg cggtttcgcg aaaggcgaag
     1741 cttttttcgg caatctcgcg cacctgcggg aagctgccgt aagcgtccaa taaattaagc
     1801 aagctggaat aagtcgcgcg aaactggctg gcgagcgcgt cgggcggcga ttttaaaagc
     1861 gtggcgatgc gcggcggatt ctgaaaattg ctcggcgcta aaatgacaaa accgacatta
     1921 tctttgccgc gcctgccggc gcgtccggtc atttgctgca attcgctggc ttgcagcgaa
     1981 cgccagccgt cattgccgcg cgtgtcggca ttgctgataa cgacggttcg cgcagggaaa
     2041 tcgactccgg cggcgacggt cgtcgtggcg aaaatcgcat ccagaagacc ggcggacatc
     2101 attttttcga tgagcagctt ccaggcggga atgtgtccgg cgtgatgcga ggcgacaccg
     2161 gcgcgaatga ccagattttt gtgtttgtgg cgaccgattt ccgggttctg ctcggcgtat
     2221 tcctgaaaga ttcggcggcg cgcctcgctg cgttcgggac tggaattttg tttcttgagc
     2281 gcgacttcca tcgccgattc gtcgcagcgc cggcgcgtcg gcataaagat aatcgcgggc
     2341 agaagattgt atgaacgcag cgaggcaatc agctgcggcg gcggaatttc gggcaatttg
     2401 aatctcatca aagattatta aagcacgaaa cgagcggaag ttttaaccgc cgagacgttg
     2461 agacgcggag attttaaaga agaatttaca gggataacag gatgaacagg gataagaaat
     2521 gattttattt atccctgttc atcctgttat ccctgtattt tttctcctct ttgtctcggc
     2581 gtctcggcgg ttaaatcagc ttattgaacg taagccgccg gaatcggaac gtcgccgttc
     2641 tgaccgaatt tatctaccgt gtaggaattc gtcgtgcttc ggaagatgta ccaatttccg
     2701 tccgtcgggc ggaagacggc gaaatccgtc ttgccgtcgc ggtcgaaatc gcccggcacg
     2761 ggcttatcgc cgcttgtgcc ccacttgaaa gctttcagcg aattgtccga gcttttccag
     2821 acataccaca cgccgtccga cgggcgaaag accgtaaaat cagcctgctt gtcgccgtca
     2881 aaatcgccga cgagcggaac gtcgccgttc tgaccgaatc taaatccctg tagattgccg
     2941 gtcgtgctcc ggcgaacata ccaggttccg tcggacgggc gaaagacggt tatatcagct
     3001 atgccgtcac cgtcgaaatc gcccgccagc ggagtatcgc cgtcagtccc ccactgaatg
     3061 gcgataaatt gattgtccgt gctgcgcaat atataccagt agccgttcga cgggcggaag
     3121 acggcgaaat ccgtcttgcc gtcgccgtcg aaatcgccgg cggcgggttt gtccgtcgcg
     3181 atgccgaact gcacggcgcg cacctgcccg ttcgagctct gagcgatata ccacgttccg
     3241 tccgaaggac gataaacggc taaatcggtt ttgcggtcgc cgtcgtagtt tccgggaacg
     3301 gcgatgtcgc cgaagcttcc gccgccgaaa cgcgtgcccg tataggtgtt gtcgctggag
     3361 cgcatcacgt gccagtcgcc ggtttccggt cgccagacgg caaggtcgct tctgccgtcg
     3421 ccgtcgaatt ccgaaggctg aacctgaatc gtgcctgtga aatcacccgt ctggctcgtc
     3481 gccggattga gataaattcg ctggcgcggc gagaatgtgt aatccatctt cgacgcccgc
     3541 agaatataat tgccaaaggt cgttacattc gaaatcgtgt aatttccgcc ggcgttggtc
     3601 gtcacggtcg cgaccggagg ctgaggattt tcttcatctt ccggataatt tctcaattcg
     3661 agcgtgacgc cttcgatgcc gatgctgttg cggtcgacga ttctgcccga aatcccgacc
     3721 ggcgcgggcg gattgaccgt gatgtcgatg aaaacgctgg ttatgaatcc gccattgtcg
     3781 cgcgcctgtg cccagagcct gtattcgccg ggacgattcg gctgccaggg cgcgttgaac
     3841 gtgccgttgt tcgattgtcc gatcgtcgtg gactgactgt tgccgaccgc cgaaacggtc
     3901 aggtgtacta tagttccgtc cgggtcggtc gcggtaacat tgaccggaat tgccgccggc
     3961 atcgtaaaga ccgcgccatt cgccggactg ttaatcgtca cgctcggcga ctgattcggc
     4021 gcggaagaga ggaaatcgat atcgagattg tcctgcgtcg cattggtcac gctgaacgaa
     4081 gccggcagga aggtcaatgt gccgacgact tgcggcgtaa tcgtataatt tccgcccgcc
     4141 ggcacattgc caaagccgta agcgccggtc gaaagggaaa tcgaagtctg attaaacgga
     4201 gtgccggttg cggtcgttcc ggtcagattg actctgatgc cctgcatcgg aaaaccgtca
     4261 ggattgtaga tgtcgccttg cagacgaacg gtcgtcacgg gcgcattgac cgtcacttcg
     4321 acgtattgcg aatccttgac cgcgccgccc gaatcgaagg cgcgcgcgaa aaagccgtga
     4381 ttgccagccg cgacgccggt taattgatgt tcgtaaggcg cggtcgtatc ggtcgcaagc
     4441 agaacggtct gcgcgccctg ccgtcgataa aactcgactt tcgtaatcga gccgccgccg
//
LOCUS       DASWNE010000064         5483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_37720, whole genome shotgun sequence.
ACCESSION   DASWNE010000064 DASWNE010000000
VERSION     DASWNE010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5483
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37720"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1428)
                     /locus_tag="VGB68_01375"
     CDS             complement(<1..1428)
                     /locus_tag="VGB68_01375"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEY0047907.1"
                     /translation="MEEIFGKSGLIAKYHKEYEFRPGQIKMAAAVLRAFEEKKHLIVE
                     AGTGTGKTLAYLVPAIAAALGQNKRIVISTGTKNLQEQLMEKDIPFLQKILPKKFTAA
                     YMKGRSNYACLYRIQKAETQPILESLDEFDSFQKVARWSRESATGDKAELIDLPENLS
                     YWRDINAKGEACIGQKCAEFENCFITRMRARAEEADIVIVNHHLFFADLNIRGNEFGR
                     VLPDYAAVIFDEAHLIEDIAADYFGFQVSSFQIDELVRDVERLPITDVALNRDLTKLS
                     AKIIGLADQFWARFAQGRSQDGRFPLVQGSFAVRTKSGEIEATALGEAYFNLDTTLER
                     LETTLDNLKDDKSEIESVVRRTRQTRFDLDFICTQAEKNYVYWLERRGKGVFLRASPI
                     DVSALLQDKLFDKTETVVMTSATLSSNGKFDFIKNRLGLDDENSDSLLAPSSFDYQKQ
                     AIVYLPKAMPDPRTPEFLQHAANEIV"
     gene            1759..>5483
                     /locus_tag="VGB68_01380"
     CDS             1759..>5483
                     /locus_tag="VGB68_01380"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HEY0047908.1"
                     /translation="MQKSSTAESRHGLLLGLFILGLVAALVILPYQFRSEAGGKKNSR
                     QELVEKTTSHEEGLENYDIRADKAAFEKIARFRQSQNRGAAQVADIRADFVKGEDALK
                     QRVPTLKVDYNLDIRTPEVIGPDVKQGRNFLTRASTVKHSEILRNFAKENNSLLGATD
                     EQLNSLKVTADYTNPDGNLSFAHLEQFINDVPVFRGEIKAGFTKQGEMIRVINNLAPG
                     LDYSSLSTDFRNPVDAVKKAAGYINHELKTSDTARNDAESNDLKVVFGTGDSATTAEK
                     MYFPTEPGVAVPAWRILIWQPVSAYYIIVDAETGTMLWRKNLTEDQTQAATYNVYANP
                     NAMINVADSPFALTPGPTAPNGTQGAAIPRTNVTRIGNEAPYTFNNNGWINDGGDRTD
                     GNAIQSGLDRDGVDGVDLNSEAVNAARNFTYTYTPFNPNTNTGDAPVPVPQTYPGSQF
                     QQGGITQMFYIANWYHDELYRLGFTEQARNYQHDNFGRGGLGNDRIRGEGQDSSGTNN
                     ANFSSGADGTRGRMQMYLWTAPNPDIDGNLDADVVIHEITHGTSNRLHGNSGGLTLNM
                     SRGMGEGWSDFYAHCLLSEPTDPINGIYTTGGYDTYQLGGVGANNFYYGIRRFPKAVM
                     AFTGGPNNRPHNPLTFADIDGSTININDGAFSPPFVGAADQVHNAGEIWSSALWEVRA
                     KMVTRLGWAVGNRKVLQLVTDGMKLAPTGPTFLTERDAILAAAAASSLAPEAAADVAD
                     VWAGFAIRGIGYSASIQNVGSGTGNARVTEAFDLPNLIQTPTFSISDAAGNNNGFAEP
                     GETLTITVPLSNNTGSTATGVTLQLVGGGSANYGTIANGATVSQQVTFTVPANTPCGS
                     VLTLTFNVNSSLGATSFTRPLIIGIPNVTFTENFDGATPPAFPAGWTSTSLQGGIPFV
                     TTTTNPDTAPNSAYAVNPTTVGGGTDLTSPSIPITASAATVSFRNRFDTEAGWDGGAL
                     EISIGVGAFQDILVAGGSFLEGGYNDLLGAGANNPLANRRAWGGNSGGYINTKVLLPA
                     AAAGQTIRLRFRFGADDNTAGTGPNPGWNVDTIQVVGAYNCPPIVANVKSRADFDGDG
                     KTDLSVFRPSDTVWYLQRSQAGFTAAQFGVASDVLTPGDFDGDGKTDLAVWRPSTGTW
                     FRINSSNGQFVGAQFGQNGDIPQPGDFDGDGRDDQAVYRNGTWYLNRSTAGFAGVSLL
                     YGVRWEINPAPTTADEKALVTLLAPPDLNRADLSDLKT"
BASE COUNT         1247 a         1507 c         1477 g         1252 t
ORIGIN      
        1 gacgatttcg tttgccgcgt gctgcagaaa ttccggcgtg cgcggatcgg gcatcgcttt
       61 cggcagataa acaatcgcct gtttctggta atcgaacgaa gacggagcga gcagcgaatc
      121 ggaattttcg tcgtccaagc cgagccggtt tttgatgaaa tcgaatttcc cgttgctcga
      181 aagcgtcgcc gaagtcatta ccacggtttc ggttttgtcg aaaagcttgt cctgcaaaag
      241 cgcggaaacg tcaatcggcg aggcgcgcag gaatacgcct ttgccgcgcc tttcaagcca
      301 gtagacgtaa tttttttcgg cttgcgtgca gataaaatcg aggtcgaaac gcgtctgccg
      361 ggtgcggcgg acgaccgact cgatttcgct tttatcgtct ttcagattgt ccagagtcgt
      421 ttccagtctt tcgagcgtcg tgtcgagatt aaaatacgct tcgccgagcg cggtcgcttc
      481 gatttcgccg gatttggttc tgacggcgaa gcttccctgg acgagcggaa agcgcccgtc
      541 ctggctgcgc ccctgcgcga aacgcgccca gaactggtcg gcgagcccga tgattttggc
      601 tgaaagcttc gtcaaatcgc gattcagggc aacatcggta atcggcagac gctcgacatc
      661 tctgaccagc tcgtcaatct gaaaactgga aacctgaaag ccgaaataat cggcggcgat
      721 gtcttcgatt aagtgcgctt cgtcgaaaat cacggcggca taatccggca gaacgcgacc
      781 gaattcgttg ccgcgaatgt tcaaatcggc gaaaaaaaga tgatgattga cgatgacgat
      841 gtcggcttct tcggcgcggg cgcgcattcg tgtaataaag cagttttcaa actcggcgca
      901 tttctgcccg atgcaggctt cgcccttggc gttgatgtcg cgccagtacg agaggttttc
      961 gggcaaatct atcagctcgg ctttatcgcc cgttgccgat tcgcgcgacc agcgcgcaac
     1021 tttctgaaat gaatcgaatt cgtccagact ctctaaaatc ggctgcgttt cggctttctg
     1081 gatgcgatac agacaggcgt aattcgagcg ccctttcata tacgcggcgg taaatttttt
     1141 cggcagaatc ttttgcagaa acggaatatc tttttccatt aattgctcct gcaaattttt
     1201 cgttccggta gagataacga tgcgcttgtt ttgtccgagc gccgccgcga ttgccggaac
     1261 cagatacgcg agcgttttgc ccgtgcccgt tccggcttcg acgattaagt gctttttctc
     1321 ttcgaacgcc cgcaagacag ccgccgccat cttgatttga ccgggacgaa attcgtattc
     1381 tttgtgatat tttgcgatga gtccgctttt tccgaaaatt tcttccattt agattatttc
     1441 tttcccaaaa atttttcgat tttatcctat ttcgatttga ctaaatcctg tctttttagt
     1501 atagttaaag tttgcaaatc accattgctc atattgctca tataatagct acgtaaggtt
     1561 actctgccgc gcttcctttt tcgggaagaa tacttagctg ccaatcatag caaattgtaa
     1621 acgtaaactt gtcctgtgtc gaagcagttt ttcgatgcgc tttatttata caaattctgt
     1681 ccttattttt aaggtaataa atcataacta cataaagtag taaaaactta tcccgttatt
     1741 agtcatagga gggcttaaat gcagaaaagt tctacagcgg aaagccgaca cggtcttttg
     1801 cttggtctgt ttattttagg attggtcgca gcattggtaa tactgccgta tcaattccgt
     1861 tcggaagctg gtggcaagaa aaattcccgg caggaattag tcgaaaaaac cacgagccac
     1921 gaagaaggtt tggaaaacta cgacattcgc gcggacaaag cggcgtttga aaaaatcgcc
     1981 cggtttcgcc agtcacaaaa cagaggcgcg gcacaggttg ccgacatcag agccgatttt
     2041 gtgaaaggcg aagacgcttt aaaacagcgc gttccgactt tgaaagtcga ttataatctg
     2101 gacatccgca cgccggaagt catcggtccc gacgtcaaac agggacgcaa ttttctgacc
     2161 cgcgcttcga cggtcaagca ttccgagatt cttcgcaatt ttgccaaaga aaataattcg
     2221 ttgctcggcg cgaccgacga acagctcaat tcgctgaaag tcacggctga ttacaccaat
     2281 ccggacggca atctttcgtt tgcgcatctg gaacagttta tcaacgacgt tccggtgttt
     2341 cgcggcgaaa tcaaagccgg tttcacgaaa cagggcgaga tgattcgcgt catcaacaac
     2401 ctcgcgccgg gcttggatta cagcagtctt tcaaccgatt tccgcaatcc ggttgacgcg
     2461 gtcaaaaaag ccgccggcta catcaatcac gaattaaaaa catccgatac ggcgcgcaat
     2521 gatgctgaat caaacgattt aaaagtcgtt ttcggcacag gcgattcggc gactacggcg
     2581 gaaaaaatgt attttccgac cgagccgggc gtggcggttc cggcgtggag aattttaatc
     2641 tggcagccgg tcagcgctta ttacatcatc gtcgatgccg aaaccggcac gatgctctgg
     2701 cgtaaaaacc tgaccgaaga ccagacgcag gcggcgacct acaacgttta cgccaacccg
     2761 aacgcgatga ttaacgtcgc cgacagcccg tttgcattaa cgccgggacc gacggcgccg
     2821 aacggcacgc agggcgcggc gattccgcgc acgaacgtga cccgaatcgg aaacgaagcc
     2881 ccttatacgt tcaacaataa cggatggatt aacgacggcg gcgacagaac cgacggtaac
     2941 gcgattcagt ccggtcttga ccgcgacggc gtcgacggcg tcgatttaaa cagcgaagcc
     3001 gtcaacgcgg cgcgcaactt tacatacact tacactccgt ttaatcccaa cacgaatacg
     3061 ggcgacgcgc cggtgcccgt gccgcaaact tatccgggaa gccaattcca gcagggcggc
     3121 atcacgcaga tgttctacat cgccaactgg tatcacgacg aactttatcg tttaggcttt
     3181 accgaacagg cgcgcaacta ccagcacgac aacttcggtc gcggcggctt gggcaacgac
     3241 cgcatcagag gcgaaggtca ggactcgtcg ggcacgaaca acgccaactt ctcgtccggc
     3301 gctgacggta ctcgcggacg tatgcagatg tatctctgga ctgctccgaa cccggacatt
     3361 gacggcaatc tcgacgccga cgtggtcatt cacgaaatca cgcacggtac gtcgaaccgc
     3421 ctgcacggaa acagcggcgg tctgacgctg aatatgtcgc gcggaatggg cgaaggctgg
     3481 tcggatttct acgcgcactg tttgctctcc gagccgaccg acccgattaa cggtatttat
     3541 acgaccggcg gctacgacac ttatcagctg ggcggcgtcg gcgcaaacaa tttttattac
     3601 ggtattcgcc gtttcccgaa agccgttatg gctttcacgg gcggtccgaa caatcgtccg
     3661 cacaatcctt tgacctttgc ggatattgac ggctcgacca ttaatattaa cgacggcgct
     3721 ttttcgcctc cgttcgtcgg cgctgccgac caggttcata acgcgggcga aatctggagc
     3781 agcgctctgt gggaagttcg cgcgaaaatg gtgacgaggc tcggctgggc tgtcggcaat
     3841 cgtaaagttt tacaattggt taccgatggt atgaagctcg ctccgaccgg tccgacattc
     3901 ctgaccgaac gcgacgctat cttagcggcg gcggcggcaa gctcgctggc accggaagca
     3961 gcggctgatg ttgccgatgt gtgggctggc ttcgccatcc gcggtatcgg ctattcggca
     4021 agcatccaga acgtcggttc gggaaccggc aacgcgagag tgacggaagc tttcgacctg
     4081 cccaacctga ttcagacgcc gactttcagc atttctgacg cggctggaaa caataacgga
     4141 tttgccgaac cgggcgaaac cttaacgatt acagttccgc tttcgaacaa cacgggcagc
     4201 accgcgacgg gcgtgacctt gcagttggtc ggcggcggct ctgcaaacta cggcacgatt
     4261 gccaacggcg caaccgtcag ccagcaggtt acattcaccg ttccggcgaa tacgccttgc
     4321 ggcagcgttc tgacattaac ctttaacgtc aacagcagcc tcggcgcaac gagtttcact
     4381 cgtccgctga ttatcggcat cccgaacgtg acctttacgg aaaacttcga cggcgccact
     4441 cctcccgcgt ttcctgcggg ctggacttcg acatcgctgc agggcggaat cccgtttgtc
     4501 accacgacga ccaatcccga taccgctccg aattcggcat acgcggtcaa cccgacgacg
     4561 gtcggcggcg gcacggattt gacttcgcct tcaatcccga ttacggcttc ggcggcgacc
     4621 gtttccttcc gcaaccgttt cgatacggaa gcgggctggg acggcggcgc gctggaaatc
     4681 agcatcggcg tcggcgcgtt ccaggatatt ctggtcgcgg gcggctcgtt cctcgaaggc
     4741 ggatataacg accttctcgg tgcgggcgcg aataacccgc tggcgaatcg tcgcgcgtgg
     4801 ggcggcaatt ccggcggtta catcaatacg aaagttctgc ttcccgcagc ggcagccgga
     4861 caaaccatcc gacttcgctt ccgtttcgga gcggacgaca atacagccgg aaccggtccg
     4921 aatccgggct ggaacgtcga tacgattcag gtcgtcggcg cttataactg tccgccgatt
     4981 gtggcgaacg tcaaatcgcg cgccgacttt gacggcgacg gcaaaaccga tttgtcggtc
     5041 ttccgtccgt ccgatacggt ctggtatctg cagcgctcgc aagccggctt taccgccgct
     5101 cagttcggcg tggcttcgga tgtcctgacg ccgggcgatt ttgacggcga cggcaaaacc
     5161 gatctggcgg tctggcgtcc ttcgaccgga acgtggttcc gcatcaattc gtcaaacgga
     5221 caatttgtcg gagcgcagtt cggacagaac ggcgacatac cgcagccggg cgatttcgac
     5281 ggcgacgggc gtgacgacca ggcggtttac agaaacggca cctggtatct gaatcgttcg
     5341 acggctggat ttgccggagt atctcttctc tacggcgtgc gctgggaaat taatcccgcg
     5401 ccgacgaccg cagacgagaa agcgctcgtg actttactgg cgccgccgga tttgaacagg
     5461 gcggatttgt ctgatttaaa aac
//
LOCUS       DASWNE010000065         2460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_355669, whole genome shotgun sequence.
ACCESSION   DASWNE010000065 DASWNE010000000
VERSION     DASWNE010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355669"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VGB68_01385"
     CDS             complement(<1..365)
                     /locus_tag="VGB68_01385"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEY0047909.1"
                     /translation="MTNKLEGLKVAILAADGFEQSELFEPKKALEEAGAGVAVVSLKS
                     GEIKGWNEKNWGDSIAVDLTVDEANAEDFDALQLPGGVMNPDKLRMSEKAVGFIKSFF
                     DAGKPVAAICHAPWTLVEA"
     gene            563..>2460
                     /locus_tag="VGB68_01390"
     CDS             563..>2460
                     /locus_tag="VGB68_01390"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY0047910.1"
                     /translation="MKRDEKKSTWKNLQDDLADRSGLAVVLVEGEDSSEICISNDNSI
                     CSILYNSEEFAPRCAEYCGRAFQTARQAGKPVLVKCHADLNFLTVPVETKDKKFAAIV
                     GRVFLKSEDYRKASERASSGDWQQFPSEEFFENVLISGSVPELEKAAKQFEKLTVAES
                     ERLNAEFGTPRSQPSAAAAGDLTPKENLPPTRRESVKNEAENSEPEETRPQIITDENG
                     LENKDRAGSESEISNSEIQNGEQILPPKPKTGDVFSGVIKDPEEAASWRSFFGSLLDL
                     EYKNACLSLLDFLTRRYGVSNLAWLERRQGHLEAIVANGKFQGQSIQIKMSAEDERLL
                     EVLRKETALELRERKTGANGEAQGVWLFPVAVGGEIRGGLVVGEGIAAEQLKRSITRF
                     IRQIAPQIEILRLRGELKRQSLLSGAVQKFNESLQAVETEDFWTHLAQFCAELMRAER
                     GSLLFFDEETQKFTVKAATGNRAGFIRSETETLGRKVAHNVFQSGKPMVVPDIKETPI
                     TPAPLEWKYKTDSFISYPITVSGQKIGVLNVTDKTSGEIYDDFDLELLNSIAPQLAVA
                     LDRVALKRKAGEYEQLSVTDALTTLYNRRYLEARLVEEINRSQRHGFPMSFMMIDVDE
                     FKPYNDRFG"
BASE COUNT          626 a          613 c          663 g          558 t
ORIGIN      
        1 tcggcttcga ccaaagtcca cggcgcgtga caaatcgcgg cgacgggctt gcccgcgtca
       61 aaaaatgatt tgataaagcc gaccgctttt tcgctcatcc gcaatttgtc gggattcatc
      121 acgccgccgg gcagttgcag cgcgtcgaaa tcttcggcat tcgcttcgtc cacggttaaa
      181 tcgaccgcga tcgaatcgcc ccagtttttt tcgttccagc ctttgatttc gccgcttttg
      241 agcgaaacga cggcgacgcc ggcgcccgcc tcttccaatg cttttttcgg ctcgaacagc
      301 tcgctctgtt cgaatccgtc ggcggcgaga atcgccactt ttaatccttc aagtttattt
      361 gtcataaaac ctccagaaat attttgcgaa ccggacgcgt ccggtttcga ttcgtgtcgt
      421 ggggaagatg gtttttcaaa caacttatat gccataatct tcttaataaa aaaacatcga
      481 agatttgtgt gaaaaaaaac ggcgattagc tgtttttacc ttttgcaagc ggcgttagaa
      541 aacctttaac tcaagcagta aaatgaaaag ggacgaaaaa aaatccactt ggaaaaactt
      601 gcaggacgat ttagccgacc ggagcggtct ggcggtcgtg ctggtcgaag gcgaagattc
      661 gtccgaaatc tgtatctcga acgacaattc gatttgcagt atcttgtata attccgagga
      721 atttgcgccg cgctgcgccg aatattgcgg cagggctttt cagacagctc gccaggcggg
      781 aaaacccgtt ctcgtaaaat gccacgccga tttgaatttt ctgaccgttc ccgtcgaaac
      841 gaaagataaa aaatttgccg caatcgtcgg cagagttttt ctgaaatccg aagattatcg
      901 aaaggcgtcg gaaagagcct cgtccggcga ctggcagcag tttccttcgg aagaattttt
      961 tgaaaacgtc ctgatttccg gctcggttcc cgaattggaa aaagccgcga aacaatttga
     1021 aaaattgacc gtcgccgaaa gcgagcggtt aaacgcggaa ttcggaacgc cccgttcgca
     1081 accgtctgcg gcggcggcgg gcgatttaac gccgaaagaa aatttgccgc cgacccgccg
     1141 tgaaagcgtg aaaaacgagg cggaaaattc tgagcccgaa gaaactcgac cgcagataat
     1201 cacggatgaa aacggattgg aaaacaaaga ccgagccggg tcggaatctg aaatctcaaa
     1261 ttccgaaatt caaaacggcg aacagatttt gccgccgaag ccgaagacgg gcgacgtttt
     1321 ttccggcgta atcaaagacc cggaagaagc cgcttcgtgg cgctcgtttt tcggctcttt
     1381 gctggatttg gaatataaaa acgcctgtct ttcgctgctg gattttttga cgcggcgcta
     1441 cggcgtttcc aatctggcgt ggctcgaacg ccgtcagggt catctggaag cgattgtggc
     1501 aaacggtaaa tttcagggac agtcaatcca aatcaaaatg tccgccgagg acgagcggct
     1561 gctggaagtt ttgcggaaag aaacagcgct cgaattgcgc gagcgaaaaa ccggggcgaa
     1621 cggcgaagcg cagggcgtat ggctttttcc ggttgccgtc ggcggcgaga ttcgcggcgg
     1681 tctggtcgtc ggcgaaggga ttgcagcgga acagcttaag cgaagtatta cacgatttat
     1741 ccggcaaatc gctccgcaaa tcgaaatttt gcgactgcgc ggagagctca aaagacagtc
     1801 tttgctttcc ggcgcggtgc agaaatttaa cgagagcctg caggcggtgg aaaccgaaga
     1861 tttctggacg catctggcgc agttttgcgc cgagctgatg cgggcggagc gcggctcgct
     1921 gctttttttc gacgaggaaa cgcagaaatt taccgtcaaa gccgcgaccg gaaatcgcgc
     1981 cggttttatc aggagcgaga ccgaaacgct cggcagaaag gtggcgcata atgtttttca
     2041 gagcggaaaa ccgatggttg tgcccgatat taaagaaacg ccgattacgc ccgcgccgct
     2101 cgaatggaaa tacaaaacag actcttttat cagctacccg attacggtca gcgggcagaa
     2161 aatcggcgtt ttaaacgtga ccgacaaaac gagcggcgaa atttacgacg atttcgattt
     2221 ggagcttttg aattcgattg cgccgcagct cgccgtcgcg ctcgaccgcg tcgcgctcaa
     2281 gcgaaaagcc ggtgaatacg aacagctttc cgtcaccgac gctttgacca cgctttataa
     2341 tcgccgttat ctggaagcca ggctcgtcga agaaatcaac cgctcgcagc gtcacggctt
     2401 tccgatgagc tttatgatga ttgacgtgga cgagtttaag ccgtataacg accgtttcgg
//
LOCUS       DASWNE010000066         2497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_2653, whole genome shotgun sequence.
ACCESSION   DASWNE010000066 DASWNE010000000
VERSION     DASWNE010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2653"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="VGB68_01395"
     CDS             complement(<1..931)
                     /locus_tag="VGB68_01395"
                     /inference="COORDINATES: protein motif:HMM:NF017039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047911.1"
                     /translation="MNSNNATPMLRQYLEIKKQYPGTILFFRLGDFYELFNEDAVTGA
                     RELEITLTARHKDSPRPVPMCGVPHHAAANYISRLVKKGYRVAICEQTEVAGQGKKLV
                     RREVVRIITPGTAIDPQLVESRENVYLASVCGAGEVFAASFLELSTGEFFTTQIFGAD
                     SWTKICSDVESFAPREILFPESLRKLIESCFGNSVQTRTPEKSNVVFITPPDSNKFSA
                     ALTGLDDWLFQTEDCENILKNQFGVRELTGFGLAGKSEAIRSAGACLRYAKDTQRAAA
                     EHIAEINYFEANDFMVLDAVTLKNLELVEARGEN"
     gene            1154..2194
                     /locus_tag="VGB68_01400"
     CDS             1154..2194
                     /locus_tag="VGB68_01400"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEY0047912.1"
                     /translation="MRAINLFAVFCLTVVIGAASVCGVFGQEPKILSDNLNPPKEAVP
                     NNITNPSLPAETKKSEDRYRVGFQDTLEITVYKHPDFGGTFNISPQGTIRLPRLNEPI
                     SVVCKTEQELQDEIAEKHKTILKNPFVNVRVVDQRSQSFAVIGAVEKPGNFYMNRRVR
                     LLELLSFAGGPDSDKAGTKLIVARTGSSSVCRQKGEDLAEVDNANLILYQYRIRDVLE
                     GKENLWMQPGDIVSVLDFDLVYVYGNVNKQGSVKLKQPLTLRQAIVEAEGFKPASKKD
                     RIRILRQKADGSDWEEFTFDLKQIDSGKVKDPFLMPNDIVAVSEDGTKTILNGLVKSF
                     TNGIPSLFYRVP"
     gene            2213..>2497
                     /locus_tag="VGB68_01405"
     CDS             2213..>2497
                     /locus_tag="VGB68_01405"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEY0047913.1"
                     /translation="MKDSREIITTKTTETTHELERPFEPPYAASGNGNYPMYRGATAQ
                     EGFQLLDYWRAIRKRLWLVLGIAVLITTLTAIYVARKPNVYEASSRVQVDL"
BASE COUNT          646 a          601 c          647 g          603 t
ORIGIN      
        1 tgttttcgcc gcgcgcttcg accagttcga ggtttttcag cgtcacggcg tccagcacca
       61 taaaatcgtt ggcttcaaaa taatttattt cggcgatgtg ttcggcggcg gcgcgctgcg
      121 tgtctttggc atagcgcagg cacgcgcccg cgctgcgaat ggcttccgat tttccggcaa
      181 gcccgaagcc ggtcaattcg cgcacgccga attgattttt caggatgttt tcgcaatcct
      241 cggtttgaaa aagccaatcg tccaaaccgg tcaaagcggc tgaaaattta ttcgaatcgg
      301 gcggcgtgat gaaaacgacg ttcgattttt ccggcgttct cgtctgcacg gaatttccga
      361 aacagctttc gattaatttc cgcaaagatt cgggaaaaag aatctcgcgc ggcgcgaaag
      421 attcgacgtc cgagcagatt tttgtccacg agtcagcgcc gaaaatctgc gtggtaaaaa
      481 attcgccggt cgaaagctca agaaaagacg cggcgaagac ttcgcccgcg ccgcagacgg
      541 aagcgagata aacgttttcg cgcgattcga ccagctgcgg gtcgatcgcc gtgccgggcg
      601 tgataatgcg gacgacttcg cggcggacga gttttttgcc ctgtccggcg acttccgtct
      661 gctcgcaaat cgccacgcgg taaccttttt tgaccagccg cgaaatataa tttgccgcgg
      721 cgtggtgcgg gacgccgcac atcggaacgg ggcgcggcga atccttgtgt cgcgcggtta
      781 aggtaatttc cagctcgcgc gcgcccgtta cggcgtcttc attaaagagt tcgtaaaaat
      841 cgccgaggcg gaaaaacaga attgtgcccg gatattgttt tttaatttcc agatactgcc
      901 gcagcatcgg cgtggcgtta ttcgagttca taaaatctga agtattcgcg tctttaatcg
      961 tttcggatag tttattccga aaaataaaca aaggacaaat gagaatgaca aagagcctgt
     1021 cgatttgcta aactatattt ttttaagtta ttttgtatca tacgtctgcc gaaaaaagga
     1081 aaaacttttc ggtcagaaag agaatcaaaa ggcttgaaat cccttcccga agtaaataat
     1141 ggagttaaag ataatgagag caattaattt gttcgcagtt ttttgtttga ccgtcgtgat
     1201 tggcgcggca tcggtttgcg gggttttcgg acaagagccg aaaattctga gcgataattt
     1261 aaatccgccg aaagaagccg ttcccaataa tataacaaat ccttccttgc cggcggagac
     1321 gaaaaaaagc gaagaccgtt accgcgtcgg gtttcaggac acgctcgaaa ttacggttta
     1381 caagcatccc gatttcgggg gaactttcaa catttcgccg cagggcacga ttcggcttcc
     1441 gcgtctcaac gaaccgattt ccgtcgtctg caaaaccgag caggaacttc aggatgaaat
     1501 cgccgaaaag cataaaacca ttctgaaaaa tccgttcgtc aacgtgcgcg tcgtcgacca
     1561 gcgctcgcaa tcgtttgccg tcatcggcgc ggtcgaaaaa ccgggcaatt tttatatgaa
     1621 tcgccgcgtt cgcctgctgg agctgctttc gtttgccggc ggtcccgatt cggacaaagc
     1681 cgggacgaaa ttaatcgtcg cccgtacggg aagctcttcc gtttgcaggc aaaagggcga
     1741 agaccttgcc gaggtcgata acgcgaattt aatactttat caatatcgaa tcagagacgt
     1801 tctggaggga aaagaaaatc tttggatgca gcccggcgat attgtttcgg tgctcgattt
     1861 cgaccttgtt tacgtttacg gaaacgttaa taaacaaggt tccgtcaaac tcaaacaacc
     1921 gctgaccctg cgacaggcga ttgtcgaagc cgagggtttt aagcccgctt ccaaaaaaga
     1981 tagaatcaga attctgcgtc aaaaagccga tggcagcgat tgggaagaat ttacgttcga
     2041 tttgaagcaa atagattcgg gaaaagtaaa agaccctttt ctgatgccga acgacatcgt
     2101 cgcggtttcg gaagatggaa cgaaaactat tttaaacggt ctggtaaagt cttttaccaa
     2161 cggaatcccc agcctttttt atcgcgttcc gtaaaatttt tgagaaaagt gtatgaaaga
     2221 tagtcgtgaa ataataacta ccaagacaac ggaaacgacc cacgagctgg agcgcccgtt
     2281 cgaaccgccc tatgccgcga gcggcaacgg aaattatccg atgtatcgcg gcgcgaccgc
     2341 gcaggaaggt tttcagctgc tcgattactg gcgggcaatc cgcaagcgtc tgtggctggt
     2401 cttgggaatc gccgtgctga ttacgactct gacggctata tatgtggcgc gcaaaccgaa
     2461 cgtttatgaa gccagctcgc gcgtgcaggt cgatctc
//
LOCUS       DASWNE010000067         8161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_143760, whole genome shotgun sequence.
ACCESSION   DASWNE010000067 DASWNE010000000
VERSION     DASWNE010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8161
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143760"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1195)
                     /gene="lptC"
                     /locus_tag="VGB68_01410"
     CDS             complement(<1..1195)
                     /gene="lptC"
                     /locus_tag="VGB68_01410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEY0047914.1"
                     /translation="MPPIQRKKVNNLKLRAKTPAYFRALALAALALTVLAIGIGFYRA
                     SGTKDFMMKGMPTQLSKDVVAVVSGFERREVEGDVVKYYIKADKATTFSDNHQELENV
                     YLEVYNPENENPDKISANRAVYIPAENKNFNAYFMGAVNIETRDGLRVKSEQIGYDKA
                     TEIAESEELVEFSRESISGKSTGAKVFVKDKRLELLSQVEINAFADENAQNNELAGAN
                     LQSARITSGRAVLQQLAGKIELEQNVNINLIPKTGAGEMSQPTDVRAEKVTAFFTDKE
                     INKLELNENVEVFIKPTEANQKYTKTRAARAVATFEGELKRVELFENVEIETTNNNSK
                     PTRIRSQYAAYEKAIDKFDLRNNVEIVTVEDEKPTTIRSQEAIYEQANGKIFLNGGAE
                     ITQAET"
     gene            complement(1249..1725)
                     /locus_tag="VGB68_01415"
     CDS             complement(1249..1725)
                     /locus_tag="VGB68_01415"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEY0047915.1"
                     /translation="MDLAVKWQELMENPFFRDIPYKVELNKFGQILMSPASNRHGILQ
                     FKVGNEIVKRKKSGVIITECSILTSEGVRVADVAWASDEFYAEFDEKTPYPKAPEICV
                     AVKSPANSRAEMDEKIRLYLEKGASEVWIVDEYAKVDFYTHTGKIKKSKIAGDFKL"
     gene            complement(1768..3477)
                     /gene="recJ"
                     /locus_tag="VGB68_01420"
     CDS             complement(1768..3477)
                     /gene="recJ"
                     /locus_tag="VGB68_01420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEY0047916.1"
                     /translation="MNKRRNVNKHDRGQVLKLSQALKISPLAAALLISRGHDAEEKAF
                     KFLNPQISDLHEPNLLRDMQKAVARIFKAVEAGEKILIWGDYDVDGTTGTVVLRKALE
                     LLGAKTGFHVPHRFTEGYGINIEKLSEAKDKGYTLVISVDTGSRAFEAADWARANGVD
                     LIVTDHHLSDETKGNPNAFAVINPNQAGCDYPDKNLAGVGVAFKLTHALLREKGKEKL
                     VKGFLKVVALGTVADMMNLTGENRAIVALGLLDLPKARNFGLRALMEVADCNSTMTSY
                     DIGFRIAPRINAAGRMDAARSVVELFEADSFEKARELAAHLDSRNRERQTIQRQIFEF
                     ALQSYLDGGGSTSQPHVAVIAGDGWHRGVIGLAASRITEKLFRPSLVISVEDGVGHGS
                     ARSIRNYHLLNALDSCVDLFEKHGGHAAACGFTIKRENIDLLREKLNAHARANLTEED
                     LTPEIRIDALVSSQTLNLDVVQELKCLEPFGIGNPKPVFATKDLYLVDEPYVMKEKHL
                     KMRLTDKTRKQFEAVWWDGVEKSKGQTLKPQTGIEIAYTPEANTWNGNTRLQLVIEDL
                     KTN"
     gene            complement(3646..4542)
                     /locus_tag="VGB68_01425"
     CDS             complement(3646..4542)
                     /locus_tag="VGB68_01425"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEY0047917.1"
                     /translation="MSFLQAIILGIVQGLTEFIPVSSTAHLVFASRWLRLYDGNAERT
                     TATIAVIQLGTLLAVIVYFLPDILSITAAFVRDHWAVLTRGRGLRFSGTNGMRPIWLS
                     EEAWLGWLVIIGSIPIGTIGLLLKEQIEGEATKNLWFIATMLIAIAVGLVIAEMVGSQ
                     RKEIRDLGISDAIIVGLSQVLALMPGSSRSGSTIMGGLFAGQKRETAARFSFLLSIPA
                     ITASGLLELKEAWHILPKEDFAPLGVGILVSAIVGYLSIWLLLAFLRKNSTAVFIVYR
                     ILLGAVILGLLYFGLLTPQANV"
     gene            complement(4736..5506)
                     /locus_tag="VGB68_01430"
     CDS             complement(4736..5506)
                     /locus_tag="VGB68_01430"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0047918.1"
                     /translation="MFWKDKIVLLTGASSGIGEALALELARRGAILGLLARRESLLQD
                     LVARCERHGKAARYFAADVTDEIAVADAADGFRREFGKIDILIANAGIGGGAKHAKDL
                     TPADFRRVIDINLNGAMNAVSAVLPQMLESGSGHLVAISSLAGFRGLPKSAAYCASKA
                     AMTAYFESVRLDVQKKGINVTIIQPGFIKTPLTSGRSNKMPFLMELEESIPHFLRAIE
                     KKKKFAAFPWQMATVVRAGKMFPAWLYDRIAGGAKYRE"
     gene            complement(5700..6149)
                     /locus_tag="VGB68_01435"
     CDS             complement(5700..6149)
                     /locus_tag="VGB68_01435"
                     /inference="COORDINATES: protein motif:HMM:NF025210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEY0047919.1"
                     /translation="MIENRQSARELLQKTRVEVAPETFFLVSLRHADWRKLLENPELS
                     PRMSAPFMIFSDKLEVTLLLDEIDFATIRHAIREAKSEGNFRLLTFDIELDFTVVGFL
                     SEILRILAEAGISIVALSAFSRDHLLIKQEDLAKALKLLGEYVEELC"
     gene            complement(6279..7940)
                     /locus_tag="VGB68_01440"
     CDS             complement(6279..7940)
                     /locus_tag="VGB68_01440"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEY0047920.1"
                     /translation="MGKVEIIPLGGIGEFGMNCMGIRYGDEMIIVDAGMGFPEETPYG
                     VDISIPNFDFLEEYRDDMTALILTHGHEDHIGALPYFLKKFNLPVYASRFTFALAERK
                     LDEHQMFDEVLLHRVKPNDVVEIGNFKIEFIHASHSLVDCFSLAITTPIGTIIHTGDY
                     KIDDTPIIGAPYDLKTLKKYGDAGVLALLSDSTNATVPGRTPSERDVVPAFEEIFEQT
                     AGRLLVSTFSSSIHRLQVIFDLAHAFDRKVCVIGRSMQRNVEIADELGLLDIPHNTQV
                     SINEARQLDDDEIVYLVTGSQGETRAALWQLATTTYKGMQIEKGDTIVLSARIIPGND
                     KAISRLIGHIYKRGGNIIEEKRRMVHVSGHASQEDIRIMTETARPKFVVPIHGEYRML
                     FRHKEFVKNHIAGYTDENVILIENGDVLELEENSARLVAHHQLERTFIDEESLGEIEY
                     ETIRERKKLAYGGAISLVVTIDRESREVVESPQITFQGVAGIDFTNGMVEEARQSIMD
                     SIKAMSKNDLHDRGSFKENLRILLKRFIQKETGTKPVIIPTIVEV"
     gene            7950..>8161
                     /locus_tag="VGB68_01445"
     CDS             7950..>8161
                     /locus_tag="VGB68_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047921.1"
                     /translation="MLTLCLNFTVINFASKFNTKIFFLLEFEFFDCRSRLCYSVKDNL
                     RGVDSPKGFVEAVSTIAQVESGSVQR"
BASE COUNT         1862 a         2159 c         2008 g         2132 t
ORIGIN      
        1 aagtttccgc ctgcgttatt tccgcgccgc cgttgagaaa aatcttgccg ttcgcctgtt
       61 cgtaaatcgc ttcctgcgag cgaatcgtcg tcggtttttc atcttcgacc gtgacgattt
      121 ccacgttgtt tcttaaatcg aatttatcga ttgctttttc gtaagcggca tattgcgagc
      181 ggattcgggt cggcttcgaa ttattgttcg tcgtttcgat ttcgacgttt tcaaacagct
      241 cgacgcgctt taattcgcct tcaaacgtcg ccacggcgcg cgcggcgcgc gttttcgtgt
      301 atttctgatt agcctcggtc ggcttgataa aaacttcgac gttttcgtta agttccagtt
      361 tattgatttc cttatcggta aaaaaggcgg tcactttttc ggcgcgcacg tcggtcggct
      421 gcgacatttc gcccgcgccg gttttcggaa tcaaattgat gttgacgttt tgttcgagct
      481 cgatttttcc ggcgagctgc tgcagaacgg cgcgtcccga agtaattcgc gcgctttgca
      541 ggttggcgcc ggcaagctcg ttattctgcg cgttttcgtc ggcgaaagcg ttgatttcaa
      601 cctggctcaa aagctccagg cgcttgtctt taacgaaaac tttcgcgccc gtcgatttgc
      661 ccgaaatgct ctcgcgcgaa aactcgacca attcttcaga ttcggcgatt tccgtcgctt
      721 tgtcgtaacc gatttgttcg gatttcaccc ggagaccgtc acgcgtttcg atgttgaccg
      781 cgcccataaa ataagcgttg aaatttttgt tttcggcggg aatgtaaacg gcgcggttcg
      841 ccgaaatttt gtcgggattt tcgttttccg ggttgtaaac ttccagatag acgttttcca
      901 gttcctggtg attgtcggaa aaagtcgtgg ctttgtcggc tttgatgtaa tatttcacca
      961 cgtcgccttc gacttcgcgg cgctcgaaac cgctgacgac cgccaccacg tctttggaaa
     1021 gctgcgtcgg cattcccttc atcataaagt ctttggttcc gctggcgcga taaaatccga
     1081 tgccgattgc cagcaccgtc aatgccagcg ccgccagcgc cagcgcgcga aaatacgcgg
     1141 gcgttttagc gcgcagcttg aggttattta cttttttgcg ttgaatcggc ggcatttcta
     1201 ttatttattg tcgtgacggg tttccggctt ctcgacggcg tttgaagctt agagtttaaa
     1261 atctccggca attttcgatt ttttaatttt tcccgtgtgc gtgtaaaaat cgactttggc
     1321 gtattcgtcg acaatccaaa cctccgacgc gcctttttcc agataaagcc ggattttttc
     1381 gtccatctcg gcgcggctgt tggcgggcga ttttaccgcg acgcagattt ccggcgcttt
     1441 cggatacggc gttttctcgt cgaactcggc gtaaaattca tcggaagccc aagcgacatc
     1501 ggcaactctc acgccttcgc tggttaaaat cgaacattcg gtaataatga cgccgctttt
     1561 ctttctcttg acaatttcat ttccgacttt aaattgcaga atgccgtgcc gattcgatgc
     1621 gggactcatc aaaatctgcc cgaacttatt aagctcgact ttatacggaa tatcccgaaa
     1681 aaaaggattt tccattaact cctgccattt caccgccaaa tccatcgtat tgcctcgtcc
     1741 agattgtagc attttcgact tcgattttta atttgtcttt aaatcctcaa tcaccaattg
     1801 caatcgcgta ttcccgttcc aggtgttcgc ttcgggcgtg taagctattt cgatgccggt
     1861 ttgcggtttt aaagtttgcc cttttgattt ttcgacgccg tcccaccaga cggcttcgaa
     1921 ttgtttgcgg gttttgtcgg tcaggcgcat tttcagatgt ttttccttca tcacgtaagg
     1981 ctcgtcgacc agatacaaat ctttcgtcgc gaaaaccggc ttcggatttc cgatgccgaa
     2041 cggttccaga cattttaatt cctgcacgac atccagattt aaagtctgcg aggagacgag
     2101 cgcgtcgatt cggatttcgg gcgttaaatc ttcttcggtc agattcgccc gcgcgtgcgc
     2161 gtttaatttt tctcttaaca aatcgatatt ttcgcgcttt atcgtaaagc cgcacgccgc
     2221 cgcgtgcccg ccgtgctttt caaaaagatc gacgcacgaa tccagcgcgt ttaataaatg
     2281 ataattccgg atgctgcgcg ccgagccgtg cccgacgccg tcttcgacgg aaatgaccag
     2341 actcgggcga aaaagttttt ccgtgatgcg cgaagccgcc aagccgataa cgccgcgatg
     2401 ccagccgtcg cccgcaatca ccgcgacgtg cggctggctc gtgcttccgc cgccgtccag
     2461 atagctttgc agcgcaaact cgaaaatctg tcgttgtatt gtctgccgct cgcggttgcg
     2521 gctgtcgaga tgcgcggcaa gctcgcgcgc tttttcaaac gaatcggctt caaaaagctc
     2581 gaccacgctg cgcgccgcgt ccattctgcc cgccgcgttg atgcgcggcg cgatgcgaaa
     2641 tccgatgtcg tagctggtca tcgtcgaatt gcagtcggcg acttccatca gcgcccgcag
     2701 cccgaaattt ctggctttcg gcaaatcgag caagcctaaa gcgacaatcg cgcggttttc
     2761 gcccgtcaaa ttcatcatat cggcgaccgt gccgagagcg acgactttca gaaagccctt
     2821 gacgagtttt tccttgccct tttcccgcaa taatgcgtga gtcaatttaa aggcgacgcc
     2881 gacgcccgcc agatttttat cgggataatc gcagcccgcc tgattcggat tgatgacggc
     2941 gaaagcgttc gggtttccct tcgtttcgtc cgacagatga tggtcggtga cgattaaatc
     3001 gacgccgttg gcgcgcgccc aatccgcggc ttcaaaggcg cggctgcccg tatcgacgct
     3061 gatgacgagc gtgtagcctt tgtctttcgc ctcgcttagc ttttcgatat tgatgccgta
     3121 gccttcggta aagcggtgcg gaacgtgaaa gccggttttc gcgcctaaaa gttccagagc
     3181 ctttcgcaga acaaccgttc cggtcgtccc gtcgacgtcg taatcgcccc aaatcagaat
     3241 cttttcgccc gcttcgacgg ctttaaaaat ccgcgccacc gccttctgca tatctcgcag
     3301 aagattcggc tcgtgcaaat cggaaatttg cggattaagg aatttaaagg ctttttcttc
     3361 cgcgtcgtga ccgcgcgaaa tgagcagagc ggcggcgagc ggcgaaattt tcaacgcttg
     3421 cgataatttt aaaacctgcc cgcggtcgtg tttgttgacg ttccggcgct tattcatttt
     3481 agattgacga ttttagattt tggattttag attggattcg gattttgctt cgcaaaactt
     3541 ttattccgga aattcaagcg agcgaataat tttttttcag agcgacaatt caaaaaaaat
     3601 aaattttctt tatccgaccg aaaattcaaa atccaaaatc gaaaattaca cgtttgcctg
     3661 cggcgttaac aagccgaaat acaataatcc taaaatcaca gctcccagta agatgcggta
     3721 aacgatgaag acggctgtcg agttttttcg caaaaacgcg agcagcagcc agatggaaag
     3781 atagccgacg atagccgaaa cgagaatgcc gacgccgagc ggcgcgaaat cttctttcgg
     3841 cagaatgtgc cacgcttctt tcagttccaa aagcccgctc gccgtaatcg ccggaatcga
     3901 gagcagaaat gaaaagcgag cggcggtttc gcgtttttgt ccggcgaaaa gcccgcccat
     3961 aatcgtcgaa ccggagcgcg acgagccggg catcagcgcc agaacctgcg aaagaccgac
     4021 gataatcgcg tcgctgatgc ccaaatcgcg gatttccttg cgctggctgc cgaccatctc
     4081 ggcgatcacc agcccgacgg caatcgcaat cagcatcgtg gcgataaacc agagattttt
     4141 tgtcgcttcg ccctcgattt gttcttttaa aagcaagccg atcgttccga tcggaatcga
     4201 gccgataatc accagccagc cgagccaggc ttcttccgaa agccaaatcg ggcgcatccc
     4261 gttcgtgccg gaaaaccgaa gccctcgccc tctggttaaa accgcccagt ggtcgcggac
     4321 aaaagccgcc gtgatgctca aaatgtcggg cagaaaataa acgataacgg caagcagagt
     4381 tccgagctga atgaccgcaa tcgtcgccgt cgtccgctcg gcatttccgt cgtaaagccg
     4441 cagccagcgg ctggcgaaca caagatgcgc ggtcgaactc accggaataa actccgtcaa
     4501 tccctggacg atgccgagga ttatcgcctg taaaaaactc ataaattagg tcggaagcat
     4561 ttcggaaaat aaaactgatt tattttgtat tgtttggcgg gattcgtcaa agagaactga
     4621 tagttgataa ctgataactg gcaactgata gttgataatt cagaatttaa cgctgagagc
     4681 ggctagctga ctgctgaccg ctatcaccta tccgcgatca gttatcagtt atcagttatt
     4741 cacgatattt agcgccgccg gcgatgcggt cgtagagcca cgccggaaac attttgcccg
     4801 cgcgcacgac ggttgccatc tgccacggga aagcggcaaa ttttttcttc ttttcgatgg
     4861 cgcgcagaaa atgcggaatc gattcttcga gctccatcag aaacggcatt ttgttgcttc
     4921 tgccggatgt cagcggcgtt ttgataaagc cgggctgaat tatcgtgacg ttgattcctt
     4981 ttttctgaac gtcgagccgg acgctttcaa aataggcggt catcgccgct tttgaagcgc
     5041 agtaagcggc ggatttcggc aaaccgcgaa aaccggcgag gctggaaatg gcgaccagat
     5101 gcccgctccc gctttcgagc atttgcggca aaacggcgga aaccgcattc atcgcgccgt
     5161 ttaaattaat gtcgattacg cgccggaaat ccgccggcgt taaatctttg gcgtgtttcg
     5221 cgccgccgcc gattccggcg ttggcaatca aaatatcgat tttgccgaat tcgcgtcgaa
     5281 aaccgtcagc cgcgtcggcg acggcaatct cgtccgtcac gtcagccgca aaataacgcg
     5341 ccgcttttcc gtgccgctcg cacctggcga ccaaatcctg caaaagactt tcgcgccgcg
     5401 ccagcagtcc gagaatcgcg ccgcgcctgg cgagttccag cgccagcgct tcgccgatgc
     5461 cgctcgacgc gccggtcaat aagacgattt tatctttcca aaacataatc tttcagactt
     5521 gacggaaggt ttgcggatag ttgacagata gtggcggaaa gttgcaggaa gttgcggaaa
     5581 gttgcaggaa gttgcggata gttaaagaaa gctatcgagg tttggaattc cgaaactgtt
     5641 tttaacttac cgcaactatc tgtcaactat ctgtcaacta tctgtcaact atttaccaac
     5701 taacaaagct cttcgacata ttcgcccaaa agcttcagcg ctttcgccaa atcttcctgt
     5761 tttatcaaga gatggtcgcg cgaaaatgcc gacaaagcga cgattgagat tccggcttcg
     5821 gctaaaatcc gcaaaatttc cgacagaaag ccgacgaccg taaaatccag ctcgatatca
     5881 aaggtcaaaa gacgaaaatt accttccgat ttcgcttccc gaatcgcgtg gcgaattgtc
     5941 gcgaagtcaa tttcgtcgag taaaagcgtc acttcgagct tgtccgagaa aatcataaac
     6001 ggcgcgctca tacgcgggct gagttcggga ttttccagca gcttgcgcca atccgcgtga
     6061 cgcagcgaaa cgagaaaaaa cgtttcgggc gcgacctcga cgcgagtttt ttgcaaaagc
     6121 tctctggcgc tttgtctgtt ttcaatcatc tatttccgtg aaatttaaat cgcatctcgc
     6181 cgcgccgggt ttaaaatctt cgcgtaaaaa gcgaaaaata aatccattcc gcacataaag
     6241 tcgcccggtt acgattttcg gcagaagcat tttaccgtct agacctcgac gattgtcgga
     6301 ataatcacgg gcttggttcc cgtttccttc tggataaatc gttttaataa aatgcgcaga
     6361 ttttccttga acgagcctct gtcgtgcaaa tcgtttttcg acatcgcctt gattgagtcc
     6421 ataatcgatt ggcgcgcttc ttcgaccatc ccgttggtaa aatcgatgcc cgccacgccc
     6481 tgaaacgtga tttgcggcga ttcgacgact tcgcggcttt cgcggtcaat cgtcacgacc
     6541 agcgaaatcg cgccgccgta ggcgagtttt ttgcgctcgc gaatcgtttc gtattcgatt
     6601 tcacccaaag attcctcgtc gataaacgtt cgctctaatt gatgatgcgc gaccagacgc
     6661 gcagaatttt cttcgagttc gagaacgtcg ccgttttcaa tcagaatcac gttttcgtcc
     6721 gtgtagccgg cgatgtgatt tttgacgaat tccttgtggc ggaaaagcat ccggtattcg
     6781 ccgtgaatcg gcacgacgaa tttcgggcgc gccgtttcgg tcataatccg tatgtcttcc
     6841 tgcgaagcgt gaccggagac gtgaaccatc cggcgttttt cctcgatgat attgccgccg
     6901 cgcttgtaaa tatgaccgat aagacgcgaa atcgctttat cgttgccggg aataatgcgg
     6961 gcggaaagca caatcgtgtc gcctttttca atctgcattc ccttgtaggt cgtcgtcgcc
     7021 agctgccaca gagcggcgcg cgtctcgccc tgcgagccgg tcaccagata gacgatttcg
     7081 tcgtcgtcga gctgtcgcgc ctcgttaatg gaaacctgcg tgttgtgcgg aatatccaaa
     7141 agcccgagct cgtccgcgat ttccacgttt cgctgcatcg agcgtccgat gacgcagact
     7201 ttgcggtcga aagcgtgcgc gaggtcgaaa atcacctgga ggcgatgaat cgaagaggaa
     7261 aacgtcgaaa ccagaagcct gcctgccgtc tgttcgaaaa tttcctcgaa cgccggaacg
     7321 acgtcgcgct ccgagggcgt tctgcccgga acggtcgcgt tggtcgaatc ggacaataaa
     7381 gcgaggacgc ccgcgtcgcc gtattttttc aaggtcttca aatcatacgg cgcgccgata
     7441 atcggcgtat cgtcgatttt ataatcgccc gtgtgaatga ttgtgccgat gggcgtcgta
     7501 atcgccagcg aaaaacaatc gaccagcgaa tgcgaagcgt gtatgaattc gattttgaaa
     7561 ttgccgattt ccaccacgtc gttcggcttg acgcgatgca gcaaaacctc gtcgaacatc
     7621 tgatgctcgt ccagctttct ttcggctaaa gcaaacgtaa agcgcgaagc gtaaacgggc
     7681 agattaaatt ttttcaggaa atacggcagc gcgccgatat ggtcttcgtg cccgtgcgtc
     7741 aggatcaaag ccgtcatatc gtcgcgatat tcttccagaa aatcgaaatt cggaatcgaa
     7801 atatcgacgc cgtaaggagt ttcttcggga aaacccattc ccgcgtccac gataatcatt
     7861 tcatcgccgt aacggatgcc catacagttc attccgaatt cgccgatgcc gccgagcggt
     7921 attatttcaa cttttcccac ctttttctta tgctcactct ctgtttaaat tttactgtta
     7981 ttaattttgc ttctaagttt aacacaaaga tatttttcct gctcgaattt gaattttttg
     8041 actgtcgaag cagactttgc tactctgtaa aagacaattt aaggggagta gatagcccga
     8101 aagggtttgt tgaagccgtc agtacaatcg ctcaagtcga aagcggttcg gttcaacgaa
     8161 g
//
LOCUS       DASWNE010000068         4076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_603016, whole genome shotgun sequence.
ACCESSION   DASWNE010000068 DASWNE010000000
VERSION     DASWNE010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603016"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VGB68_01450"
     CDS             complement(<1..228)
                     /locus_tag="VGB68_01450"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEY0047922.1"
                     /translation="MQRKFFLTLAIILSAMTFSITAAAAPQFSVYLNGRQEVPANNSP
                     GSGTCFVTLNATETQVSVECRYRNLTSNVVGA"
     gene            1065..3338
                     /locus_tag="VGB68_01455"
     CDS             1065..3338
                     /locus_tag="VGB68_01455"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY0047923.1"
                     /translation="MSNKTLPLFKFDDFYLDIEERCLTFQDQPVSLTPKAFQTLVVLL
                     RNHGKVVEKEYFLNEVWADTFVEESTLAQNILTLRKTLSRFQKDKEFIATVPRRGYQF
                     VGDVQEIPGGDEEIIVVEKRTLTHLIAEQSEIHDSTDTNSDKISVNLRQTLLKNGFSP
                     RGAIFVGLLAFLAFTIGYFASFGLKGYQSFAESQFKKFRVDTIVADADIRNSTISPNG
                     KYLALVQVKNGVQSLHLRQIENGNSVEIVPRINGRFIGAVFSPRDEQIYYSVNENLEL
                     NKPAVSTLYKVSVLGGASQKILHDIDSPVAISPDESRLAFVRRNLERKETALILTDIE
                     GKNERSLAIRQTENGFTNGGVSWSPDGKLLSATVFQRENNRVSAQVAVVDAESGEQRT
                     ISRENWISAGQTVWLKDGSGILAVAYGAKSPSLNDELWVVSYPDGKSRFVTNGINGNY
                     GISLNHATNSIVAVESNKFACFLTAPVDNLFKNTHVLTTISDESPLPFGADWTNDGRI
                     VYSATDGGNADIFTISEDGSERKQLTSDPSAEISPKLSADGRFLIFMSNRTGQMDVWR
                     ADSNGTNTIQLTTNGNVKDSIISPDGETVFYLVQDPESMVETLWRVSINGENPVKLTD
                     RTTRSPRISPDGKTIACYISSSETRAMKLALVSAETGEVLKHPATPQSDDIPFLDWSK
                     DGENLFVVLQRGKPFSFWKLPLNGSQPEQLREWENDAIFRLAISRNGERVFYEVGNQL
                     NDVVQFQSLDSDSKPGF"
     gene            complement(3481..3999)
                     /locus_tag="VGB68_01460"
     CDS             complement(3481..3999)
                     /locus_tag="VGB68_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047924.1"
                     /translation="MQKIPKEELLKPEKKWTGWLLLCLFVSILGACVFTAGFFQDSAS
                     NPESDFSKRNFLFIFGGSWMLVWGVIFLLLFGVYYAAKKRRETLLLHGQYGSAVILKI
                     DETGDSEGSPLPVKMKLEIYLEGFAPYQVEETMEVPILKISQPQVGSTVEVLAAPAVP
                     GVRPGAVKLLYK"
BASE COUNT         1065 a          991 c          956 g         1064 t
ORIGIN      
        1 cgctccgacg acattcgaag ttaaatttcg atatctgcac tcgaccgaga cctgcgtttc
       61 ggtcgcgttg agcgttacga aacaagtgcc gctgccgggc gaattgttcg ccggaacttc
      121 ctgcctgccg tttaagtaga cggaaaattg gggcgccgcc gccgccgtta tcgaaaacgt
      181 cattgccgac aaaatgattg ccagcgttaa gaaaaatttt ctctgcattt tacttgatac
      241 tccttaagtc ttgaactgtt gctaatgtta ggttttagaa cgaaattaaa acgctcacct
      301 tcttacggaa attttcctgt tatttaaaga aagaaattat tcttgctcac aggctataat
      361 tcgaattcga taaaccgagg ttgatgtttt gacccaagag gttatttctt taagtgattc
      421 acgttcgtga agcagccgtt cggtcaatgt ttttaatgat tcaactcgat tttcaaacgg
      481 tttgagattt ttcagatttt ctaaaataga ttccgcagct ctcaaactct ttttgctctg
      541 agacaactat ttattgattc atctcatctt ttttaatgct aaaataagca taaagatctt
      601 agacaaattc gcggcgcttc gagattttgg taacgaaagt gaatgcgccg attttccatc
      661 atcgaacgaa tcgcttcgac gctgtgtccg gtcagcagcg ccgcttgctg aagcggcagc
      721 atactgactt ctcgtccgca atcttcgcac caagcccgaa cgaacgtccg atttcgctta
      781 atgattaagg tttcgctggt ttcaataaaa acttccgtcc gggtttttgc cattgatttc
      841 cttttttaat ccggctgcaa ggatttgtct aaaatgttta aatattttga atgaggaaaa
      901 ccaaagtgct gttgttgctg ttgttgtcac ctaacttcta aaacagcaaa ttgttatcgt
      961 ctccctcatc cgtactcgac gataagaaac tttcaacttg aaagtcttga aagttacttg
     1021 aaagttactt tgaaattttt atgaaagact tttgaaatac gcttatgagc aacaaaacat
     1081 tacctttatt caaattcgac gatttttatc tggacatcga agagcgatgt ctgacgtttc
     1141 aagaccagcc cgtttccctg actccgaagg cttttcaaac gctcgtcgta ttgctgcgta
     1201 atcacggaaa agtggtcgag aaagaatatt tcctcaacga ggtctgggcg gatacctttg
     1261 tcgaggaatc cacattggcg caaaatatcc tgaccctcag aaaaactctc agccgctttc
     1321 agaaggacaa ggaatttatc gcgacggttc cgcgccgcgg ttaccagttt gtcggcgacg
     1381 tgcaggaaat cccgggcggc gatgaagaaa taatcgtggt tgaaaaacgc acgctgacgc
     1441 atctcatcgc cgaacaatcg gaaatccacg attcgacgga tacgaattcg gacaaaattt
     1501 ccgtcaacct gcgtcaaacg cttttgaaaa acggcttttc gccgcgcggc gcgattttcg
     1561 tcggcttgct ggcgtttctg gcttttacaa tcggatattt cgcgtctttc ggcttgaagg
     1621 gataccaaag ctttgcggaa tcgcagttta aaaaatttcg cgtcgatacg attgtcgccg
     1681 acgccgacat ccgaaactcg acgatttccc cgaacggaaa gtatctcgcg ctggtgcagg
     1741 ttaaaaacgg cgttcaatct ctgcatctgc ggcaaatcga aaacggcaat agcgtcgaga
     1801 ttgttccgcg aatcaacgga agatttatcg gcgcggtttt ttcgccgcgc gacgagcaaa
     1861 tttattattc cgtcaatgaa aatctcgaat tgaacaaacc cgccgtctcg acgctctaca
     1921 aagtttcggt gctcggcggc gcttcgcaaa aaatcctgca cgacatcgac agcccggtgg
     1981 cgatttcgcc cgacgaaagc cgtctcgcct tcgttcgccg taacctcgag cgcaaggaaa
     2041 cggcgctgat acttaccgat atcgaaggca aaaacgaacg cagcctggcg attcgccaga
     2101 cggaaaacgg atttacaaac ggcggcgtgt cctggtcgcc ggatgggaaa ttactttccg
     2161 cgaccgtttt tcagcgtgaa aacaaccggg tatcggctca ggttgcggtc gtcgacgccg
     2221 aatccggcga acaaagaaca atttcacgcg aaaactggat ttcggcgggt caaaccgtct
     2281 ggctcaaaga cggcagcgga attctcgccg tcgcttacgg ggcgaaatcg cccagcttaa
     2341 acgacgaact ctgggttgtt tcttacccgg acggcaaatc ccgattcgta accaacggca
     2401 tcaacggaaa ttacgggatc agcttgaacc acgcgacaaa ttcgattgtc gccgtagaat
     2461 cgaataaatt tgcctgtttc ctgaccgctc cggtcgataa tctatttaaa aatactcacg
     2521 ttttaacgac aatttcggat gaaagcccgc tgccgttcgg cgcggattgg acaaacgacg
     2581 gaaggattgt atattccgca acggacggcg gaaatgcgga cattttcact atcagcgagg
     2641 acggcagcga gcgaaaacaa ctgacttccg acccgagcgc cgaaatctcg ccgaaacttt
     2701 ccgccgatgg acgctttttg atatttatgt cgaatcgcac cggtcagatg gacgtttggc
     2761 gggctgactc gaatggaacg aacacaatcc agctgacgac aaacgggaat gttaaagact
     2821 cgataatttc gcctgacggc gaaacggttt tttatcttgt tcaggaccct gaaagtatgg
     2881 tcgagacact atggcgagtt tcgattaacg gcgaaaatcc tgtgaaacta accgaccgga
     2941 ctactcgctc gccgcgcatc tcgcctgacg gaaaaacgat tgcctgttat atttcaagtt
     3001 ccgaaacgcg cgcgatgaag cttgcgcttg tttcagccga aacgggcgaa gtgttgaagc
     3061 atccggcgac tccgcaatcg gacgacattc cttttctcga ttggtcaaaa gacggcgaaa
     3121 atttgttcgt cgtgctgcag cggggaaagc ctttcagttt ttggaagctt cctttaaacg
     3181 gcagccagcc cgaacaactg cgcgaatggg aaaacgatgc gattttcagg ctcgcgattt
     3241 ccagaaacgg tgaaagggtt ttttatgaag ttggaaacca gttaaatgac gtggtgcagt
     3301 ttcaaagcct cgattcagat agtaaacccg gcttttaatg gcggctgttc ttttaaagac
     3361 ttcgtcgcgc gttcggcggc gtaatgcgct taatcgtgcc ggctcgccga tttcatcatc
     3421 aaagagagct taaaggctcg cggcagggat tttctttcgg cgggaaatcc ggctatattt
     3481 ttatttgtaa aggagcttaa ccgcacccgg gcgaacgccg ggaacggctg gcgcggctaa
     3541 aacttcgacg gtcgagccga cttgcggctg cgaaatctta agaatcggaa cttccatcgt
     3601 ttcttcgact tgatacggcg caaatccttc gagataaatt tccagcttca ttttgaccgg
     3661 aagcgggctg ccttcgctgt cgcctgtttc gtcgatttta agaatgacgg cgctcccgta
     3721 ttgcccgtgc agcagcagag tctcgcgacg tttttttgcc gcataataaa caccgaaaag
     3781 cagaagaaaa atcacgcccc aaaccagcat ccagctgccg ccgaaaataa aaaggaaatt
     3841 tcgctttgag aaatcacttt caggattcga cgccgaatcc tgaaaaaatc ccgccgtaaa
     3901 tacgcacgcg cccagtatcg agacaaataa acagagcaaa agccagccag tccacttttt
     3961 ttcgggtttt agtaattctt ctttaggtat tttttgcatt aattaatttc tcccgtttat
     4021 ggtatttccg gctcgcacgg tttcggcgac ggattatctt cgtaaaaatt gctcac
//
LOCUS       DASWNE010000069         2187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_285103, whole genome shotgun sequence.
ACCESSION   DASWNE010000069 DASWNE010000000
VERSION     DASWNE010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285103"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..>2187)
                     /locus_tag="VGB68_01465"
     CDS             complement(207..>2187)
                     /locus_tag="VGB68_01465"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY0047925.1"
                     /translation="GLLIFQDLAIVVMVMLVPMLGGAGGGTLDIVWALSKALLIIAAV
                     LLVARRIMPKVLEMVAKTCSPELFLLSVIAICFGTAYLTSLAGVSLSLGAFLAGLLVS
                     ESRFSQHALGETLPLQILFSATFFVSVGMLLDVHFLIANLPLILAAIAVVLIIKILTT
                     GISLAALGYKLPPAAACALMLAQIGEFSFVLERAGREVGLTPMNWGAAGSQTFIAATV
                     VLMVATPFLMKIGDALSEKIEKKTNKASLPAESEIAEQVSSHAVDLEDHVIVAGYGEA
                     ARYLVRVLSGSSIPFIITTLSPEGANEAESESMSVVRGDYSKQFLLDAVGIAKAKMMV
                     VADDNISMAHRTTMVARQLNPTMRIVVRTRYISDAEHLTEAGADVVIAEELESIVQLF
                     GEVLRDYRISSAQIENYEELARQNGYSALLNETASDDQSVFACQPGEDCFDTRTIVVR
                     PSMTFADKPLFSLNVLENDGLHIQSVSRNGQTIEDLNQDFIIEPGDEIILSGLTEAFI
                     RNAEIFRAANENKPESAAARPFSRGENVLTAEPVKTKKFAEAKTNPYKYVDTEKAVLF
                     KPKINERACGHLKQARPVFPSADGCEDCLRTGDAWVHLRICLSCGHTGCCDSSKNKHA
                     TKHFHSSAHPLIKSLEPSEEWGYCYPDEKIYQTAG"
BASE COUNT          520 a          589 c          559 g          519 t
ORIGIN      
        1 ttttatcact atttattgtc ccgcaaacga gtgtttttga cactggttat cgcgacgtag
       61 ttctgatcgg cggagccgga taaaagccat tttcgctccc gaattatatt ttataaaatt
      121 gccgctttga ttgctctgac ggcgcgaaaa aagcaatcga actgttttgc gtgactgtcg
      181 gcagggaaaa gacctgctta aaaacgtcaa ccggcggttt gataaatctt ctcgtccggg
      241 tagcagtagc cccattcctc gctcggctct aaagatttga tgagcgggtg cgcgctcgaa
      301 tgaaaatgtt tcgtcgcgtg tttgtttttc gacgaatcgc aacagcccgt atgaccgcac
      361 gacaggcaga ttctcaaatg cacccaggcg tcgcccgttc gcaggcaatc ttcgcagccg
      421 tccgcgctcg gaaatacggg tcgcgcctgc tttaaatgac cgcaagccct ttcgttaatc
      481 tttggtttga ataaaacggc tttttcggtg tcaacgtatt tgtatgggtt tgtttttgct
      541 tctgcgaatt ttttagtttt gacgggttcg gcggtcaaaa cattttcacc gcgcgaaaac
      601 gggcgtgctg cggcgctttc aggtttgttt tcattcgccg cacgaaagat ttcggcgttg
      661 cgaataaacg cttcggttaa accggataaa ataatttcgt cgcccggctc gataataaaa
      721 tcctgattta aatcttcgat tgtctgaccg ttccggctga cggattgaat atgcagcccg
      781 tcgttctcca gaacgttcaa tgagaaaagc ggtttgtcgg caaaagtcat cgacgggcga
      841 acgacaatcg tgcgcgtatc gaagcaatcc tcgcccggct ggcaggcaaa aaccgattgg
      901 tcgtcactcg ccgtttcgtt gagcaatgcg gaatatccgt tttgtctggc tagctcttcg
      961 taattctcga tttgagccga tgaaattcgg taatcgcgca aaacttcgcc gaatagctga
     1021 acgattgatt ctaattcttc ggcaatcacc acatcggctc cggcttcggt taaatgctcg
     1081 gcgtccgaga tgtagcgcgt tctgaccacg atgcgcatcg tcggattcag ctgccgcgcg
     1141 accattgtcg tgcggtgcgc catcgatata ttatcgtcgg cgacgaccat cattttcgct
     1201 ttcgcgatgc cgaccgcgtc gagcagaaac tgcttgctgt aatcgccgcg cacgaccgac
     1261 atgctttccg attcggcttc atttgcgcct tcagggctta aagtcgtaat gatgaacgga
     1321 atgctcgaac cgcttagaac ccggacgaga tagcgcgccg cctcgccgta accggcgacg
     1381 atgacgtggt cttccaaatc gacggcgtga gaggaaacct gttccgcaat ttcggattcc
     1441 gcgggaagac ttgccttgtt ggttttcttt tcaatttttt cggaaagcgc gtccccgatt
     1501 ttcatcaaaa acggcgtggc gaccatcagc accacggtcg ccgcgataaa cgtctgcgag
     1561 ccggctgcgc cccagttcat cggcgtcaaa ccgacttcgc gccccgcgcg ttctaaaaca
     1621 aaggaaaatt cgccgatttg cgcgagcatc aaagcgcacg ccgccgccgg cggcagctta
     1681 tagccgagcg ccgccagact gattccggtc gttagaatct tgattattaa aacgaccgca
     1741 atcgcggcta aaatcagcgg taaattggca atcaaaaaat gaacgtcgag cagcattccg
     1801 accgaaacga aaaacgtcgc gctgaacaaa atctgcagcg gcagcgtctc gcctaaagcg
     1861 tgctggctga aacgactttc cgaaaccagc aatccggcga gaaaagcgcc gagtgaaaga
     1921 ctgactccgg cgaggctggt cagataagcc gtgccgaaac agatggcgat caccgaaagc
     1981 aggaaaagct ccggcgagca ggttttcgcc accatttcca aaactttggg cattatccgc
     2041 cgggcaacga gcaaaacggc ggcgataatc aataacgctt tggagagcgc ccagacgatg
     2101 tccaatgttc cgccgcccgc gccgccgagc atcggcacga gcatcaccat cacgactatc
     2161 gccaaatcct gaaagataag cagtccg
//
LOCUS       DASWNE010000070         3449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_2820, whole genome shotgun sequence.
ACCESSION   DASWNE010000070 DASWNE010000000
VERSION     DASWNE010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2820"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..1812)
                     /gene="purH"
                     /locus_tag="VGB68_01470"
     CDS             complement(169..1812)
                     /gene="purH"
                     /locus_tag="VGB68_01470"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEY0047926.1"
                     /translation="MSDLRKIRRALISVSDKTGIVEFAKELASFGAEIISTGGTAKNL
                     REAGLNVTEVSEITAFPEMMDGRVKTLHPKIHGAFLALRDNVEHVAAMQKHGIEPVDL
                     VVVNLYPFEETVAKDDVPLEEAVENIDIGGPAMIRSASKNWRDVAVVTDARLYESVLR
                     EMKETGGSLGAQTRRRFAALAYTRTASYDLAISSYLAKQLNDADLQFLEPFNPLADLV
                     FIEADAGEDESETETFSQFQSIELAKVADLRYGENPHQKAALYETNEAGGIAKAEQLH
                     GKEMSFNNYVDAEAAWNLVCDFEELACAIIKHTNPSGVGTGAANEEAYRRALATDPVS
                     AFGGIVAFNRKVDGAVAKSVNEVFTEVVVAPDFDGEALEIFRTKKNLRVLRAEKSQAQ
                     NKLEYKQISGGMLVQDKDLYRVTENDFKIVSDRQPSAEEIRALLLAWKVCKHVKSNAI
                     VFANEFQTVGVGAGQMNRVDSVRIAAMRAERFALPLKNAVLASDAFFPFRDNVDEAAK
                     FGVSAIVQPGGSMRDEESIRAANEHGLTMVFTGFRHFKH"
     gene            2210..>3449
                     /locus_tag="VGB68_01475"
     CDS             2210..>3449
                     /locus_tag="VGB68_01475"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047927.1"
                     /translation="MCFINLRLRQTIFLFSLFFLFAAAGSAREIELTVKFLSFSPDAK
                     VRVEGTMPDGVDAGKSWSFLQNYADVTNLGERIENLKFLDARGRPIQFKKLAPGEFEA
                     EQVPASFQYDVKTAISQQLTAAAHVSWISDTHGLLMLGDLLPQNLQNFSAKITFELPE
                     NWRVATSETKRSEKAFSVRNVEKAIFLIGKNWREQTKQLEKNELSLATFGEWQFTDAE
                     ALEIAGTIIADYRKIFGEIPFPKTQVLLLPFPQAANFERWRAETRGSTVTIISAPTTF
                     KSAAVNRLHEQLRHEIFHFWIPNSLNLSGNYDWFYEGFTLYKALKTGVRLNQIRFDDF
                     LSTLGRAFDLTEALSRRQSFSLIEASQKRWINSNELIYAKGIVVAFLADATLLRESRG
                     KRDLDAIFQEVFQKHRAPNQK"
BASE COUNT          832 a          906 c          843 g          868 t
ORIGIN      
        1 ggaaaatgcg tccgagcgtt ccttctaaaa acttaatgct tattttttac tgtattttta
       61 aacgaatgtc aaacattatt taaagtaatc ctttaacttt ttacaatttc cttacgcttg
      121 agcgaccgaa gcgatagctc gcgcgcgaac aggagctttt gccgcagact aatgtttgaa
      181 atgtcgaaag ccggtaaaga ccatcgtcaa gccgtgttcg tttgccgccc ggatgctttc
      241 ctcgtcgcgc atcgagccgc ccggctgaac gattgccgac acgccgaatt tcgcggcttc
      301 gtcgacatta tcgcgaaacg ggaaaaacgc gtcggaagcc agaaccgcat tttttaaagg
      361 cagggcgaat ctttcggcgc gcatcgccgc aattctgacc gaatcgacgc ggttcatctg
      421 tcccgcgccg acgccgacgg tctgaaattc gttggcgaaa acaatcgcgt tcgatttcac
      481 gtgcttgcag actttccacg caagcagcag cgcgcggatt tcttcagcgc tcggctggcg
      541 gtcggaaaca attttaaaat cgttttccgt cacgcgatac aaatccttgt cctgcacgag
      601 cattccgccg gaaatctgtt tgtattcgag cttgttttgc gcctggcttt tctcggcgcg
      661 caaaacgcgc agattttttt tcgtccggaa aatttccagc gcttcgccgt cgaaatccgg
      721 cgcgacgacg acttcggtaa aaacctcatt gaccgatttg gcgaccgctc catcgacttt
      781 ccggttaaaa gcgacgatgc cgccgaaagc cgaaaccggg tcggtcgcca gcgcgcgccg
      841 gtacgcttcc tcgttcgccg cgccggttcc gacgccggac ggatttgtat gcttgataat
      901 cgcgcaggca agctcttcaa aatcgcaaac cagattccaa gccgcttccg cgtcgacata
      961 gttgttaaaa gacatttcct tgccgtgcag ctgctcggct ttggcgatgc cgcccgcttc
     1021 attcgtctcg taaagcgcgg ctttctggtg cggattttcg ccgtagcgaa ggtctgcgac
     1081 tttggcgagc tcgattgatt ggaactgcga aaaagtttcc gtttcgcttt cgtcctcgcc
     1141 cgcatcggct tcgatgaaaa ccaaatcggc gagcgggtta aacggctcta aaaactgtaa
     1201 gtccgcgtcg ttcaactgtt tagccagata gctcgaaatc gccaaatcgt agctcgccgt
     1261 ccgcgtataa gccagtgcgg caaagcgccg gcgcgtttgt gctccgagcg agccgccggt
     1321 ttccttcatt tcccgcaaaa cgctttcgta aagccgcgca tcggtgacga cggcgacgtc
     1381 gcgccagttt ttcgacgccg agcgaatcat cgccggaccg ccgatgtcga tattttcgac
     1441 ggcttcttcc aatggaacat cgtctttcgc cacggtttct tcaaacgggt aaagattgac
     1501 gacgaccaaa tcgacgggct cgatgccgtg cttctgcatc gcggcgacgt gctcgacgtt
     1561 atcgcgcaaa gccagaaacg cgccgtgaat tttcgggtgc agagttttga cgcgcccgtc
     1621 catcatttcg ggaaaagcgg tgatttccga aacttccgtg acgtttaatc cggcttcgcg
     1681 caaatttttc gccgttccgc cggtcgagat gatttccgcg ccgaaactcg ctaattcctt
     1741 agcaaattcg acgattccgg ttttatccga aacgctgatc agcgcgcgtc tgatttttct
     1801 taaatcgctc ataatatttc gtttaaaaat aaaaatagcg gtctcgctcg accgccggtt
     1861 attcaattca atttaaagac aattaaaacc ttcgaacctg aaaatccagg ttccgccgaa
     1921 aattcagctt taccgaaccc gctttgccga gtcatccgcc tagaagaaaa aacgtccgaa
     1981 aagcagcagg ctcaacagaa aagcaagcgt ataaatgacg actttaatga tgctcataat
     2041 aattttcccg tctgaaacca atgagaatta taaaaacaga taacgaacaa agcgtcattt
     2101 tagtttaaaa attctttcct ttacaagtca gcgaataaat ttttccggca aagcccgcaa
     2161 aattcccgtt tccgtctgca aacggcttgt gctaaatttt atctgcaaga tgtgttttat
     2221 aaatctcagg ctgcggcaga caattttttt attttccctg tttttccttt tcgccgccgc
     2281 cggcagcgcg cgggaaatcg aattgacggt gaaattttta tcgttttcgc cggacgccaa
     2341 agtgcgcgtc gaaggaacga tgccggacgg cgtcgacgcc ggaaaaagct ggtcgtttct
     2401 ccaaaattac gccgatgtca ctaatttagg cgaaagaatc gagaatttaa aatttctcga
     2461 cgcgcgcggt cgtccgattc aatttaaaaa actcgcgccg ggcgaattcg aagccgagca
     2521 agttccggct tcatttcaat acgacgtgaa aacggcaatt tcgcagcaat taacggcggc
     2581 ggcgcacgtt tcgtggattt ccgacacgca cgggcttttg atgttgggcg atttgctgcc
     2641 gcagaatcta cagaatttca gcgcgaaaat aacctttgaa ctgccggaaa actggcgcgt
     2701 cgcgacaagc gaaacgaagc ggagcgaaaa agctttcagc gtcagaaacg ttgaaaaagc
     2761 aatttttcta atcggcaaaa actggcgcga gcagacaaaa cagcttgaaa aaaatgaatt
     2821 gagcctggcg accttcggcg aatggcagtt tacggacgcc gaggctctgg agattgccgg
     2881 gacgattatt gccgactacc ggaaaatttt cggcgagatt ccttttccga aaactcaggt
     2941 tttgcttttg ccttttccgc aggcggcaaa tttcgagcgt tggcgagcgg aaacgcgcgg
     3001 ctcgaccgta acgataattt ccgcgccgac gacctttaaa tccgcggctg tcaatcgttt
     3061 gcacgagcag ctgcgacacg agatttttca tttctggatt ccgaattcgc tgaatttaag
     3121 cggaaattac gattggtttt acgaaggctt tacgctttat aaagccttga aaaccggcgt
     3181 ccggcttaac cagatacgtt ttgacgattt tctctcgact ttggggcgcg cctttgattt
     3241 gaccgaggcg ctttcgcgcc gccagagttt ttcgctgatt gaagcctcgc aaaaacgctg
     3301 gattaattcc aacgagctta tctacgccaa aggcatcgtc gtcgctttcc tggcggacgc
     3361 gacgcttttg cgcgagagcc gcggcaagcg cgatttggac gcgatttttc aggaagtttt
     3421 tcaaaaacat cgcgcgccga atcaaaagg
//
LOCUS       DASWNE010000071         1607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_638956, whole genome shotgun sequence.
ACCESSION   DASWNE010000071 DASWNE010000000
VERSION     DASWNE010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638956"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(382..458)
                     /locus_tag="VGB68_01480"
     tRNA            complement(382..458)
                     /locus_tag="VGB68_01480"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(422..424),aa:Arg,seq:tcg)
     gene            complement(554..1027)
                     /locus_tag="VGB68_01485"
     CDS             complement(554..1027)
                     /locus_tag="VGB68_01485"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY0047928.1"
                     /translation="MNFLKIGLLLSALTLFIVACTQTSPVNNATTGTANNRNVVANTA
                     PAANQSPSNPAKQTESLPAGQATDMTSASETYQTICAKCHKENGEGGEVSIDGKKLKV
                     PNFKSERMKNDEDEDFIDAIANGIPEDGMPAFKDRLNEAQIKNLVQYIRKDIQGK"
     gene            1248..>1607
                     /locus_tag="VGB68_01490"
     CDS             1248..>1607
                     /locus_tag="VGB68_01490"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0047929.1"
                     /translation="MNTKKILIGGEWREAKEDFEVKNPFTSESIAGVFSADASENEEA
                     VAIAESAAKEMRNPARYEIAEGLRKIAAQIEKRKEEFARTIALEAAKPIMTARGEVER
                     GIATFSWAAGEAERFAGE"
BASE COUNT          454 a          355 c          359 g          439 t
ORIGIN      
        1 tttcaatact gtatctttta ttcttcccta aaactttgac gtaatcattt tcattattgc
       61 aggaatccca atcaattatt ccgaagatat atctatttcc gaaacctgcg agtttatcaa
      121 ccacctcttg cacttggttg caatttcctg aattaccaat tcccgaagat ataaaattaa
      181 gagatatttc agatatggac acgcttcaat cgtcttaacg cttgatacgt atagccacgt
      241 cttaccgaca atgcagaaaa gcgcgaccga taagctcaaa aaaatgatga ttgaaagata
      301 gttttttttt aagttggcac acatctggca cacaaaagaa aaagggcaat caatttgatt
      361 gcccttaact attgaaaata ttggtacacc cggcaagatt cgaacttgcg accctctgat
      421 tcgaagtcag atactctatc cagctgagct acgggtgcgt cctgaatttt aaattttaga
      481 tgctcattcc ggcatttgca aatttgcggt tcaaaaactt gcagcagccg acgggcgctt
      541 tgtcatttct cgattatttt ccctgaatgt ctttgcggat atattgaacc agatttttaa
      601 tctgcgcttc gtttaagcgg tctttaaaag cgggcattcc gtcttcggga atcccgttgg
      661 ctatggcgtc gataaagtct tcgtcctcgt cgtttttcat ccgctcgctt ttaaagttcg
      721 ggacttttaa ttttttgccg tcgatgctga cttcgccgcc ttcgccgttt tccttgtgac
      781 atttcgcgca gatggtttga tatgtttcgc tcgccgaagt catatcggtc gcctgacctg
      841 ccggcaatga ttcggtttgc tttgcgggat tggagggcga ttgattggcg gcgggcgcgg
      901 tgtttgcgac gacatttcga ttattcgccg tgccggtcgt cgcattattg accggactcg
      961 tctgcgtgca ggcgacgata aacagcgtca gagccgataa aagcagaccg atttttaaaa
     1021 aattcataga ttctccctga taaaaatttg ataacaacga ttggaaagtt taacgtaaaa
     1081 taaaaagcaa acgcaattat tttttataaa accctttgcg caaaacacgc aaaccggtaa
     1141 cagccggtta ataagattga cagggattag tcaggataaa ttcaaataaa atactaacca
     1201 atcttattaa cctttaaaaa tcagcgtccg attttcagag aacctttatg aacacaaaaa
     1261 aaatcttaat cggcggcgaa tggcgcgagg caaaagagga ttttgaagtc aaaaatccgt
     1321 ttacgagcga atctatcgcc ggagtttttt cggcggacgc ttcggaaaac gaagaagccg
     1381 tcgccattgc cgaaagcgcg gcgaaagaga tgcgaaatcc ggcgcgctac gaaatcgccg
     1441 aaggattgcg aaaaatcgcc gctcaaatag aaaaacgcaa agaagaattt gcgcgaacca
     1501 tcgccctcga agcggcgaaa ccgattatga cggcgcgcgg cgaagtcgag cgcggcatcg
     1561 cgacattcag ttgggcggcg ggcgaagccg aaagatttgc gggcgag
//
LOCUS       DASWNE010000072         4306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_249865, whole genome shotgun sequence.
ACCESSION   DASWNE010000072 DASWNE010000000
VERSION     DASWNE010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249865"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..1746)
                     /locus_tag="VGB68_01495"
     CDS             complement(187..1746)
                     /locus_tag="VGB68_01495"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HEY0047930.1"
                     /translation="MHLFRSNLLFLVLFLLISLSVPAQQNSDCLVSVTFLHVNDVYQF
                     APVDGGTRGGLARLLTLKKQLKEQNPNTIFTMGGDTISPSVESRTYKGAQMIDAWNAV
                     GIDYAVFGNHEFDVKTDVLLQRMKESKFQWLGANVFDKRAQKIFADTPPFVIREFEGV
                     KVGIIGFLLPETKQTSSMEEHLEVLDFCQTAKNFVPKMREAGAKTIVGLTHLSMEQDK
                     QLAKCADFDLILGGHEHTLLQSSSNGTPIFKMTADARELGKFNLNINKKTGEVETMDW
                     EIIPVTSKIADAPEFNEAVAKYNNLLQELAKPVGETSETLDALSFSNRNKETNIGNFI
                     ADAYRKAVGADVALVNGGSVRADLTYNPGALTRRDVLSILPFNNPILKVEVSGKLLRE
                     ILEHGVARSAEDNEPGRFPQISGMSFIFDVSRRSGNRVTEILVGGKPLDEKKNYTLAT
                     SDFLVTRGGDGYVMFQNAKVLTKAEQAPKDSDALEKAITNSPNKTIAPKLENRIVKIG
                     QSGVKGKLSCP"
     gene            1861..2916
                     /locus_tag="VGB68_01500"
     CDS             1861..2916
                     /locus_tag="VGB68_01500"
                     /inference="COORDINATES: protein motif:HMM:NF014675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HEY0047931.1"
                     /translation="MSVSPARAETLPLPQREFRAVWMTSVDNIDFPTRKNLSVEEQKK
                     ELLRNLELARELKLNAVVFQVRPMCDALYESKIEPWSEFLTGKMGKPQAFDPLRFLIA
                     EAHRRGILVHAWFNPFRAYHPAAKTVSENHISKRRPDLVRKYGKYLWLDPTDKEVQDY
                     SLSVVLDVVRRYDVDGVHFDDYFYPYAEKDASGNKIEFPDDRNWQKHLRAYERLKPRN
                     AARYPALNRDDWRRANVNRFIEAVGREIKKIKPEILYGVSPFGIWQPVPEKGIEGFNA
                     YAELYADSRKWLRDGTVDYLAPQLYWETARQGVSFPVLHDWWKAQNVKNRHTGRESRL
                     IASAQAQILPPPKSPIK"
     gene            2811..3302
                     /locus_tag="VGB68_01505"
     CDS             2811..3302
                     /locus_tag="VGB68_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047932.1"
                     /translation="MESPEREKPAHWAGIAPYRIGSGANFTAAEIANQIKLTRSSPES
                     PGAIHFSFKSLRNDLGGIQKTLSDGVYAKDALIPPTVWIKARKPLAPKVKITRDEKFV
                     RAAWTEQGARKAFWFVVYAKDRNGWSYSILPASRKSVSLSADRKIEKIIVTSVDRLGN
                     ESR"
     gene            complement(3384..4178)
                     /locus_tag="VGB68_01510"
     CDS             complement(3384..4178)
                     /locus_tag="VGB68_01510"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HEY0047933.1"
                     /translation="MSEYGKSAPLSYNKYLKVPELIQLQETLSDPQSHDEMLFIVIHQ
                     TYELWFKQILHEIDATIKWLDENRPFRANHRLRAVDGIEKVLVSQIHLLETMAQIGFL
                     EFRDKLNPASGFQSTQFRELEFVSGAKDENILKHFSDDEFAFRRLTGRFDAPSLADVF
                     WNILRKNGFAAETNDERVASIVEILTHPEKYADFFIMQDLLIEHDENIALWRYHHVLM
                     VERMLGMKRGTGGSEGAGYLRKTLDKKFMPELWEARTHLKSASSEP"
BASE COUNT         1034 a         1095 c         1099 g         1078 t
ORIGIN      
        1 gcttcgctga aataaacgct cagtcgaatg ataaatagaa aaggaacggc atttattttg
       61 ccgttccttt ttttatgatg cggttcattc cgaaccaaaa cgcgcgcttt ccggtcacga
      121 ttgagtttta accataaaat agttgccggg gcaatcgggg cgcgtcccgg cattttttta
      181 agtgtctcac ggacagctca atttcccttt gacgcccgac tgaccgattt taacgatgcg
      241 gttttccagt ttcggcgcaa tcgttttgtt cggcgaattc gtaatcgctt tttccagcgc
      301 gtccgaatcc ttcggcgctt gttcggcttt cgttaaaact ttcgcatttt ggaacatcac
      361 atagccgtcg ccgccgcgcg tgacgagaaa atcagaagtc gccagcgtgt aatttttctt
      421 ttcgtccaga ggttttccgc cgaccagaat ttcagtcacg cgatttcccg accgcctgga
      481 aacgtcgaat ataaagctca tacccgaaat ctgcgggaaa cgtcccggct cgttgtcttc
      541 cgcactgcgc gcgacgccgt gttccaaaat ttcgcgcagg agcttgccgg aaacttcgac
      601 tttcaaaatc ggattgttaa acggcagaat cgaaagcacg tcgcgcctgg tcaaagcgcc
      661 cggattatat gtcaaatccg cgcgaacaga gccgccgttg accagcgcca cgtcggctcc
      721 gacggctttg cgataagcgt cggcgataaa atttccgata ttcgtctcct tgttgcggtt
      781 ggaaaaggaa agcgcgtcca gcgtttcgct cgtttcgccg acgggtttcg ccagctcctg
      841 caaaagattg ttgtatttgg cgacggcttc gttaaattcg ggcgcgtcgg cgattttgct
      901 ggtgacggga ataatctccc aatccatagt ttcgacttcg cccgtttttt tgttgatatt
      961 taaattaaat ttgccgagtt cgcgcgcgtc ggcggtcatt ttaaaaatcg gcgtgccgtt
     1021 tgaagaagac tgcaaaagcg tgtgttcgtg accgcccaga atcaaatcga aatcggcgca
     1081 ttttgccagc tgcttgtcct gttccatcga aagatgcgtc agcccgacaa tcgttttcgc
     1141 gcccgcctcg cgcattttcg gcacgaaatt tttggcggtc tggcaaaaat cgagcacttc
     1201 cagatgttct tccatcgagg aagtctgctt ggtttcgggc agcagaaaac cgatgatgcc
     1261 gactttcacg ccttcaaatt cacgaatgac gaacggcggc gtgtcggcga aaattttctg
     1321 cgcgcgtttg tcaaagacgt tggcgccgag ccactggaat ttcgattctt tcattcgctg
     1381 gagcaaaacg tcggttttca cgtcgaattc gtgattgccg aagacggcgt agtcgattcc
     1441 gaccgcgttc caggcgtcga tcatctgcgc gcctttataa gtgcgggatt cgaccgaagg
     1501 cgaaatcgtg tcgccgccca tcgtgaaaat cgtgttcgga ttttgttctt tcaattgctt
     1561 ttttaaagtc agaagccgcg ccagcccgcc gcgcgttccg ccgtcgacgg gcgcgaattg
     1621 ataaacgtcg ttgacgtgca gaaacgtcac gctgaccagg caatcggaat tttgctgagc
     1681 cggaaccgaa agcgaaatca gcagaaaaag aactaaaaac aaaagatttg agcgaaaaag
     1741 gtgcattgcg aatctccctt aaaaatttca aattgaatta tacttacttt tgccggattt
     1801 gagcatcagc cggacgggaa aatattaatg cgctttttta taatttttct tttctttttt
     1861 atgtcggttt ccccggctcg cgccgaaacg ctgccgctgc cgcagcgcga gtttcgcgcc
     1921 gtctggatga cgagcgtcga caacatagac tttccgacaa ggaaaaatct ttcggtcgag
     1981 gaacaaaaaa aagagctttt gcgaaacctg gaactggcgc gcgaattgaa attaaacgcc
     2041 gtcgtttttc aggtgcgccc gatgtgcgac gcgctctacg aatcgaaaat cgagccgtgg
     2101 tcggaatttc tgacgggcaa aatgggaaag ccgcaagcct tcgacccttt gcggtttttg
     2161 attgcggaag cgcaccggcg cggaattctg gttcacgcgt ggttcaatcc ctttcgcgcc
     2221 tatcatccgg cggcgaaaac cgtctcggaa aatcatattt ccaaacgccg ccccgatttg
     2281 gtgcgaaaat acggcaaata tttgtggctc gacccgaccg ataaagaagt tcaggattac
     2341 agcctgagcg tcgttttaga cgtcgtccgg cgttacgacg tggacggcgt gcactttgac
     2401 gattattttt acccgtatgc ggaaaaggac gcgagcggca acaaaatcga atttcccgac
     2461 gaccgaaact ggcagaaaca tctgcgcgca tacgagcggc tcaaaccgcg caatgccgcg
     2521 cgttatccgg ctttaaaccg cgacgattgg cggcgggcaa acgtcaaccg gtttatcgaa
     2581 gcggtcgggc gcgaaatcaa aaaaataaag ccggaaattc tctacggcgt ttcgcctttc
     2641 ggaatctggc agccggttcc cgaaaaagga atcgaaggct ttaatgctta cgccgagctt
     2701 tacgccgatt cgcgcaaatg gctgcgcgac ggaaccgtcg attatctcgc gccgcagctc
     2761 tactgggaaa ccgcgcggca aggcgtatcg tttccggttc tgcacgattg gtggaaagcc
     2821 cagaacgtga aaaaccggca cactgggcgg gaatcgcgcc ttatcgcatc ggctcaggcg
     2881 caaattttac cgccgccgaa atcgccaatc aaataaaatt aacgcgaagc tcgcccgaat
     2941 cgcctggcgc gattcatttc agtttcaaat ctctgcgcaa cgatttgggc ggaattcaaa
     3001 aaacgctgag cgacggcgtt tatgccaaag acgcgctgat tccgccgacc gtctggataa
     3061 aggcgcgaaa accgctcgcg ccgaaagtaa aaatcacgcg cgacgaaaag ttcgtccgcg
     3121 ccgcctggac ggagcagggc gcgcgaaaag ctttctggtt tgtcgtctac gcgaaagacc
     3181 gaaacggctg gagttattcg attctccccg cttcgcgaaa aagcgtttcg ctttccgccg
     3241 accgaaaaat cgaaaaaatc atcgtaacga gcgtcgatag attgggaaac gaaagtcgat
     3301 gacagcgagc agcgagcggt aagcggcgag cagtttggat tcaaggattc aatgttgaac
     3361 ttcaatacta tactgctcgc cgcttacggc tcactgctcg ccgacttcaa atgcgtgcgc
     3421 gcttcccaga gttcgggcat aaattttttg tctaaagttt ttcgcaggta gcccgcgcct
     3481 tccgagccgc ccgtgccgcg cttcataccg agcattcttt cgaccatcaa aacgtgatga
     3541 taacgccaga gcgcgatgtt ttcgtcgtgt tcgatgagca aatcctgcat gatgaaaaag
     3601 tctgcgtatt tttccggatg cgtcaggatt tcgacgattg acgcgactct ttcgtcattt
     3661 gtttcggcgg caaacccgtt ttttcgtaaa atattccaga acacgtcggc gaggctcggc
     3721 gcgtcgaatc ttccggtcaa acgccggaaa gcgaattcgt cgtcggagaa atgtttcagg
     3781 atattttcgt ctttcgcgcc ggagacgaat tccagctcgc gaaactgcgt cgattgaaaa
     3841 ccgctcgccg ggttcagttt gtcgcggaat tccagaaaac cgatttgcgc catcgtctcc
     3901 agcaggtgaa tctgcgagac cagaactttt tcgatgccgt cgacggcgcg taatctgtga
     3961 ttggcgcgaa acgggcgatt ttcgtcgagc catttaatgg tcgcgtcaat ctcgtgcagg
     4021 atttgcttaa accataattc ataggtttga tgaatgacga taaaaagcat ctcgtcgtgg
     4081 ctttgcgggt cggacaaagt ttcctgcaat tgaatcaatt ccgggacttt caagtatttg
     4141 ttgtatgaaa gcggcgcgct ttttccgtat tcgctcatag atttaaaaac taaataaaaa
     4201 cttaataaga actgtcttat cttcgttttt tatggattcg gtgtcaattt tgccgccgga
     4261 ttggctggat tttacaaaaa aagtaaaaaa gctcttgaca gaggct
//
LOCUS       DASWNE010000073         7272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_73537, whole genome shotgun sequence.
ACCESSION   DASWNE010000073 DASWNE010000000
VERSION     DASWNE010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7272
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73537"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VGB68_01515"
     CDS             <1..572
                     /locus_tag="VGB68_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY0047934.1"
                     /translation="ETTANAMIFTWYLLSQNPEAEAKFHAELDALFPNGDAPKPQDYP
                     RLKYTEAVLAEAMRLFPPAWATGRLAIEEHEINGFTIPKGSLVIASMFVTQRDERFWE
                     AAEKFQPERWLKENAVKEAGQKFVYFPFGGGVRRCIGEQFAWMEGVLLLATLGRKWKL
                     RLQPEQKIVLQPLMTLRSKYGMKMRIEKR"
     gene            569..721
                     /locus_tag="VGB68_01520"
     CDS             569..721
                     /locus_tag="VGB68_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047935.1"
                     /translation="MIRYNVFFDNIQPDNFRPMIVSEHEISADAARQVSAEAGLSAGK
                     LLSIRA"
     gene            complement(804..2234)
                     /locus_tag="VGB68_01525"
     CDS             complement(804..2234)
                     /locus_tag="VGB68_01525"
                     /inference="COORDINATES: protein motif:HMM:NF024677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAFs-binding domain-containing protein"
                     /protein_id="HEY0047936.1"
                     /translation="MSKEESGDVKGTCFVVMGFGKKTDFESGRTLDLDKSYRSIIKPA
                     VEAAGLKCIRADEIVHSGLIDVPMYEQLLNADVVIADLSTSNKNAFYELGIRHALRPY
                     TTVIIAEDGIKTFPFDISHVVVRQYRHLGDAIDYDEALRFQKQLTEAVVEVFNKDPRQ
                     KDSPVYTFLNNLNPPALAAAVQNAIQTAVTEAGDSNKTENSDNPAGDKQSKAASEQEK
                     NDSAQNHSILMQQIEDAQTEGNFIKAKNLLEAVRDMMKTQCPMKAEDPDIIQRLALAT
                     YKSEFPTEKDALTQARDLLTILQPATSNDTETLGLWGAVHKRLWNLTRQAAYLDQAVR
                     AYERGFHLRNDYYNGINFAYLLNERAANAQEKAEAIADFVQARRIRREVLSICENWLR
                     DNPVPDAQKADARTIKRCRKSRYWVLATMAEAWLGLGDEAKSNELAQAANEQMPNEWM
                     KDSAREQIEKLKALLRYSPLVNIKTD"
     gene            complement(2613..3290)
                     /locus_tag="VGB68_01530"
                     /pseudo
     CDS             complement(2613..3290)
                     /locus_tag="VGB68_01530"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
     gene            complement(3309..5957)
                     /locus_tag="VGB68_01535"
     CDS             complement(3309..5957)
                     /locus_tag="VGB68_01535"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEY0047937.1"
                     /translation="MKIRQKAKGERQKFARIFSFFSFVGGISALARPCVLILLLSVGV
                     FAQNNFENRTISDIQISFTGADRDISAAEQFRLIAQSILIGDYSTVKIRETLQALYNT
                     EKIVSAQVEATSVGENQVSLRFIIKRKTQAEKVNIRIADSVGTEVTEDQLLLRTNILN
                     QGGNVTEQTLRTNAESIQTYLRDRGYYNAEVTYTQTPLENANRVAVTFQVNPKTQARV
                     GNFAIAIEGFADAKLRDDLNLKTGEFFSRRRLEEDVAKIRQAILKQGYLAPRLGEPRV
                     TFDPEKNSINIELTGEIGPKVNVKVEAGQEEVGEGTQTRLLPIRREGTLDYSAIIEGG
                     RRLRNHFQERGYFFVEVNAVCSVTPPFPENQADPSENNTEALCGSLASEDLDARVVDV
                     NYQVDLNRRLRLEDIRIEGTDKITIEDIATVLKTQRASLLGLIPRLGYGRGYTSNEIL
                     EDDRKQVEAIMRELGYRRAKATVRQGVSPTGDDLIITFVINEGPVTRISDIDIESNKA
                     FDDDRLKQELPPVIGREYSRARARNGVQKLTEFYANQGYFDARVTFSIVELQQAGTLN
                     AAGEEEVKIVYNIENEGKKVFVNRILINGNDITRRAAILKAITLREGEILKAVDIAQS
                     EQFLYATDAFVRVEIKAEEAGETRGGDKQRDIIINVEEQKPRILNYGGGFSTDGGPFG
                     SFDIRHTNLFGKLQQGGALARMSRRRQLVQLNYLDPRFIGDGRNRWAPLNITAQYQRD
                     STVTRFFRSVFDQGTMGIVQRVDEDGNPIDIFGNETGEPTINRLTVTAETQRTMNLKT
                     RSILFVRYRFEDVRLFNTESLLIGELLAPDARIRTSGFGATFVRDTRQNCNRGNSLLE
                     LISKGDLGDPCRYSPTDPTKGSYLTA"
     gene            complement(6010..>7272)
                     /locus_tag="VGB68_01540"
     CDS             complement(6010..>7272)
                     /locus_tag="VGB68_01540"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEY0047938.1"
                     /translation="AGIANLSVRVAGSHADARLTGTAQVENASVSTFVGSERLNFNRI
                     NGRILFTSNQAQIEQVVGNLGGGQVVASGGALLEGLQLQRFRLDLRGTNVTAPLPKDF
                     VTTGDAEITISGERRGDAYESLIAGRVRMRRAVYSRDIDLADVIGNRREASISEGTGA
                     PFLGIPRLALTIQGENALVVRNNLANLTASADLVVTGDADFPVITGRITANSGTIFFR
                     NDRYEVQRGTLEFPAQADRAPFINLQAETEIQGYQIFVNLLGELSDFESLNATVRSNP
                     ALPQADVVSLITSGNLANTGDGIPTLASTGINTAAEILADALINNPARKATDKLFGLN
                     RFEIDPILSGRRLNPTARLTVGRQINRNLLITYSTNLSEDQNQVLALEYRVSNRLSFI
                     AQYEQGSTTNVTNSRDNFSFEIRLRKRF"
BASE COUNT         1577 a         1985 c         1917 g         1793 t
ORIGIN      
        1 acgaaacgac cgccaacgcg atgattttca cctggtatct gctctcgcaa aacccggaag
       61 cggaagcgaa atttcacgcc gagttagacg cgctttttcc gaacggcgat gcgccgaaac
      121 cgcaagatta cccgcgtctg aaatacacgg aagcggtttt agccgaagcg atgcggcttt
      181 ttccgcccgc ctgggcgacg ggcaggctgg cgattgagga gcacgaaatc aatgggttta
      241 cgattccgaa aggctctttg gttatcgcca gtatgtttgt cacgcagcgc gacgaaagat
      301 tctgggaagc ggcggaaaaa tttcagcccg aacgctggct gaaagaaaac gccgtcaagg
      361 aagccggaca aaaattcgtt tattttcctt tcggcggcgg cgtccgccgc tgtatcggcg
      421 agcagtttgc ctggatggaa ggcgttttgc ttctggcaac tctcggcaga aagtggaagc
      481 tgcggctgca gcccgaacaa aaaatcgttt tgcagccgct gatgacgctt cgctcgaaat
      541 acggaatgaa gatgcggatt gagaaaagat gattcgatac aacgtttttt ttgataatat
      601 tcagcctgat aattttcgac cgatgatcgt ttcggagcac gaaatttcgg ctgacgcggc
      661 gcgtcaagtt tcggctgaag ccgggcttag cgccggaaaa ttattgtcga ttcgcgccta
      721 agccgtcaat tttcatttcc gccggttatt actaaacaag tcgataaaac ctatcaatcc
      781 tgacctttgc attagctgtc gggctaatcg gtcttaatat ttaccaaagg agagtatcga
      841 agcagcgctt tcagtttctc aatttgctcg cgcgccgaat ctttcatcca ttcgttcggc
      901 atttgctcat ttgcggcttg cgccagctcg ttcgattttg cttcgtcccc gagccccagc
      961 caggcttcag ccatcgtcgc caatacccaa tacctgcttt tccggcagcg cttaatagtt
     1021 cgcgcgtcag ctttttgggc gtcgggaaca ggattatctc tcagccaatt ttcacaaatt
     1081 gaaagcacct cccggcgaat tcggcgcgcc tgtacaaagt cggcgatagc ttccgccttc
     1141 tcctgcgcgt tggcggctcg ctcattaaga agataggcga aattaattcc gttataataa
     1201 tcatttcgca aatgaaatcc tctttcgtag gcgcggacag cctggtcgag atacgccgct
     1261 tgcctggtta aattccagag acgcttatgt accgctcccc agagcccgag cgtttcggtg
     1321 tcgttggatg tcgccggctg caggatagta agcaggtccc gcgcctgcgt caaagcatct
     1381 ttttcagtcg gaaattcact tttataagta gccaaagcca gacgctgaat tatatcgggg
     1441 tcttccgctt tcataggaca ctgcgttttc atcatgtcgc gaacggcttc gagcagattt
     1501 tttgccttga tgaaattacc ttccgtctgc gcatcttcta tttgctgcat caggatgctg
     1561 tggttctgcg cgctgtcgtt tttctcctgc tcgctcgctg ctttgctttg tttatcgcct
     1621 gccggattat ccgaattctc tgttttattg gaatcgccgg cttctgtaac ggcagtttgt
     1681 atggcattct gcacagcggc ggcaagcgcc ggaggattga gattatttag aaatgtatag
     1741 accggactat ctttttgtct ggggtcttta ttgaatacct cgaccacggc ttccgtcagc
     1801 tgtttttgaa atctcaacgc ttcatcgtaa tcaatagcat cgcccaaatg ccgatactgc
     1861 cggacgacaa cgtggcttat atcaaaggga aaagttttta ttccgtcttc ggcaatgata
     1921 accgtcgtgt acggtcgcag cgcgtggcgt atgcccagtt cgtagaaagc atttttattg
     1981 gaggttgaaa ggtcggcgat aaccacatcg gcgtttaaga gctgctcgta catcggcacg
     2041 tcgataagac cggaatgcac tatctcgtca gcccggatac attttaaacc tgccgcttcg
     2101 actgccggtt tgattatgct tctgtaagat ttatcgaggt cgagcgtgcg ccccgattcg
     2161 aaatcggtct ttttgccgaa tcccattacg acgaagcatg tccctttgac atctcctgac
     2221 tcttctttag acataaacaa atctcctgtt ttaaagtttc gtgaaattag ctttatcaaa
     2281 gccgacgctc ctggaaaatc cgcgtaaacg ggctgttaac gagctgtttc ggaaagtatg
     2341 acgaatcggc tcgccggtcg ccttccggtt aggtatcggc attgtccacc ttttgccgat
     2401 gtttttcaag gttaagcggg aatcccgaac gccgctgcgc ccggcgcaaa cgccgccgga
     2461 ttcctgatga tttttcccga acctcatatt cagagattca atcagacaaa tgaaaaaggc
     2521 gcgctcgctc ccggcgcgcc tttttcaaag cggatttaac ttgttcaata caggtctgat
     2581 ttcccgccgg cgaaaaccgc ggaaaggaac gattaaaatg tctgagtaaa gcggaagtga
     2641 agctgcgtct gtcttaattg ataaatcgcg ttcgtgccgt ttatctgcgg aatcaaaaat
     2701 cgcggcggat ttgtcagata gccgaaatca atcgccaccg cgccgccgat cggcgttttg
     2761 attcttaaac ccagcccgac cgtatgcgtc cagacggcgc gcagattact gcgcagaaca
     2821 tcattcgggt cgacgtccgg cggattaaaa atatcgccga cgcgccggaa aacgttgccg
     2881 ccgtcgtaaa acggcacagc ttggatgccg tccgtgaccg gcaggcgcgc ttcgagattg
     2941 acaatcgcca gcgcgttgcc gccgaacggc acggtaaacg ggcgcagaac aacctgctcg
     3001 ccctcgctgt tgcgaaacgt tccctgcgga atgatgacga ttcgcggtcc agccgattcg
     3061 gcatcgaagc ctctgaggct ggtcgagccg cccgagaaaa atctttcgct gatcggcagg
     3121 ctgccgttca aagcctcgaa tccggcggga aatcgctgct cgtcggcaaa gacgctcgcc
     3181 aagcctaaaa tcgcgcgtcc cgccagagtc gcgtttttca atctcggaaa tgtgtaataa
     3241 gtttgaaagc tgccctgaaa cttgtgaaaa ccgatattcg cgcccaagac cgggacggaa
     3301 acgttgtatt acgccgtcag atagcttcct ttggtcgggt cggtcgggct gtagcggcac
     3361 gggtcgccga ggtcgccttt ggaaatcagc tccagaagcg agttgccgcg attgcagttt
     3421 tgccgggtgt cgcggacgaa tgtcgcgccg aagccggacg tgcgaatccg cgcgtcgggc
     3481 gcaagcaatt ccccaatcaa aaggctttcc gtattaaaca gccggacgtc ttcgaatcga
     3541 taacggacaa acaaaatgct gcgcgttttc aggttcatgg tgcgctgcgt ctccgccgtc
     3601 acggtcagac gattaatcgt cggctcgccg gtttcgttgc cgaaaatatc aatcggattg
     3661 ccgtcttcgt ccactcgctg gacgatgccc atcgttccct ggtcgaaaac cgagcggaaa
     3721 aatctcgtca cggtcgaatc gcgctggtac tgcgccgtaa tatttaaagg cgcccagcga
     3781 tttctgccgt cgcctataaa acgcgggtcg agataattta attgaacgag ctgccgccgc
     3841 cgactcatac gcgccagcgc gccgccctgc tgaagcttgc cgaaaagatt ggtgtgacga
     3901 atatcaaacg agccgaacgg tccgccgtcg gtcgaaaaac cgccgccgta atttaaaatt
     3961 cgcggctttt gttcctcgac gttgatgata atgtcgcgct gcttgtcgcc gccgcgcgtc
     4021 tcgcccgctt cctcggcttt gatttccacg cgcacgaaag cgtccgtcgc gtaaagaaac
     4081 tgctcgctct gcgcgatgtc gacggctttt aaaatttcgc cttccctcag cgtgatggct
     4141 tttaaaatcg ccgcgcgcct ggtgatgtcg ttgccgttaa tcaggatgcg gttgacgaaa
     4201 acttttttgc cttcgttttc gatgttatag acgattttga cttcttcttc gccggcggcg
     4261 ttgagcgttc ccgcctgctg caattcgaca atcgaaaacg tgacgcgcgc gtcgaaatag
     4321 ccttgattgg cgtaaaattc ggtgagcttc tgaacgccgt ttctcgcccg cgcccgcgaa
     4381 tactccctgc cgatgaccgg cggcaattcc tgtttcagac ggtcgtcgtc aaacgctttg
     4441 ttgctttcga tatcaatgtc gctgattctg gttaccggac cttcattgat gacgaaagtg
     4501 ataatcaaat catcgcccgt cggcgagacg ccctggcgaa ccgtcgcctt tgcacggcga
     4561 taaccgagtt cgcgcataat ggcttcgacc tgcttgcggt cgtcttccag aatctcgttg
     4621 ctcgtgtagc cgcgcccgta accgagccgc ggaatcagac cgagaagcga ggcgcgctgc
     4681 gttttcagaa cggtggcgat gtcttcgatt gtgattttgt ccgtgccttc gatgcggatg
     4741 tcttcgagcc gcaggcggcg attcagatcg acctgataat ttacgtccac gacgcgcgcg
     4801 tccaaatctt cgctcgccag gcttccgcac aaagcttccg tattgttttc cgacggatct
     4861 gcctggtttt ccggaaacgg cggcgtcacc gagcaaaccg cgttaacttc gacaaagaaa
     4921 taacctcttt cctgaaaatg atttctgagc cgccgcccgc cttcgataat cgccgagtaa
     4981 tccagcgttc cctcgcggcg aatcggcaaa agccgcgtct gcgttccctc gccgacttct
     5041 tcctgaccgg cttcgacctt gacgtttacc ttcggaccga tttcgcccgt cagctcgata
     5101 ttaattgaat ttttttcggg gtcgaacgtg acacgcggct cgccgagtcg cggcgcgaga
     5161 tagccttgtt tgagaatcgc ctggcggatt ttcgccacgt cttcttccag acggcggcgc
     5221 gagaaaaatt ctccggtctt cagattcaaa tcgtcgcgga gcttcgcgtc ggcaaagcct
     5281 tcgatggcaa tcgcaaaatt tccgacgcgc gcctgcgtct tcggattgac ctgaaacgtg
     5341 acggcgacgc ggttcgcgtt ttcgagcggc gtctgcgtat aggtcacctc ggcgttgtaa
     5401 taaccgcggt cgcgcagata ggtttgaatc gactcggcgt tggtgcgaag cgtctgctcg
     5461 gtcacgttgc cgccctgatt taaaatgttg gtgcgcagca aaagctggtc ttcggtcact
     5521 tccgtgccga cggaatccgc gatgcgaata ttgacttttt cggcttgcgt tttgcgcttg
     5581 ataataaagc gcaggctgac ctgattttcg ccgaccgacg ttgcttcgac ctgcgccgaa
     5641 acgatttttt cggtattgta gagcgcctgc agagtttcgc ggattttgac cgtcgaataa
     5701 tctccaatca ggatgctttg agcgattaag cgaaattgct cggcggcgga aatgtcgcgg
     5761 tcagcgcccg taaagctgat ttgaatatcg gaaatggtgc gattctcgaa attattctga
     5821 gcgaaaacgc caaccgacag aagcagaatc aaaacgcacg ggcgggcgag cgcagagatg
     5881 cctccgacaa aagaaaaaaa ggaaaaaatc cgcgcaaatt tttgcctttc gccttttgcc
     5941 ttttgcctta tcttcattcg gttcatccct gcttgaattt ttttcagttc tccgtggttc
     6001 aatttttttc taaaatctct ttctcagacg aatctcaaaa ctaaaattat cgcggctgtt
     6061 cgtgacgttg gtcgtcgagc cctgttcgta ttgcgcgatg aacgaaaggc gattggaaac
     6121 gcgatattcc aaagccagaa cctgattctg gtcttccgat aaattggtcg aatacgttat
     6181 caataaattc cggttaatct gcctgccgac cgtcaggcgc gccgtcggat taaggcgtct
     6241 gcccgacaga atcgggtcaa tctcgaaacg gttcaagccg aaaagcttgt cggtcgcctt
     6301 gcgcgccgga ttgtttatca gcgcgtcagc cagaatttcc gccgccgtgt tgattccggt
     6361 cgaggcgagc gtcggaattc cgtcgcccgt gttggcgaga tttcccgacg tgatgagcga
     6421 gaccacatct gcctgcggca gcgccggatt agaccgcacg gtcgcgttca aagattcgaa
     6481 atcggaaagc tcgccgagca gattgacaaa aatttgataa ccctgaatct cggtttccgc
     6541 ctgcaaattg ataaacggcg cgcggtcggc ttgcgcggga aattccaaag ttccgcgctg
     6601 gacttcgtaa cggtcgttgc ggaagaaaat cgtgccgctg tttgccgtga ttctgccggt
     6661 aatcaccgga aaatccgcgt cgcccgtgac gaccaaatca gccgaagccg tcagattcgc
     6721 cagattgttg cgcacgacca gcgcgttttc gccctgaatc gtcagagcca ggcgcggaat
     6781 gccgagaaac ggcgcgcccg tgccttcgga aatcgatgct tcgcggcgat tgccgataac
     6841 gtcggcaagg tctatatctc tggaatacac ggcgcggcgc attcgaactc tgcccgcaat
     6901 cagagattca taagcgtcgc cgcgacgctc gccgctgata gtaatttccg cgtcgcccgt
     6961 cgtgacgaaa tctttcggca gcggcgcggt cacgttagtg ccgcgcaggt cgagccggaa
     7021 acgctgcagc tgcaagcctt cgagcagcgc gccgcccgaa gcgacgacct gcccgccgcc
     7081 caagttaccg acgacctgtt caatctgcgc ctgatttgaa gtgaacaaaa tccgaccgtt
     7141 gatgcggtta aagtttagac gctcgctgcc gacaaaagtc gaaaccgaag cgttttcgac
     7201 ctgcgcggtt ccggtcaggc gcgcatcagc gtgcgagccg gcgacgcgca cggaaagatt
     7261 ggcgattccg gc
//
LOCUS       DASWNE010000074         2992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_37973, whole genome shotgun sequence.
ACCESSION   DASWNE010000074 DASWNE010000000
VERSION     DASWNE010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37973"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VGB68_01545"
     CDS             complement(<1..381)
                     /locus_tag="VGB68_01545"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY0047939.1"
                     /translation="MSRPEKNEYAEYYETYVSLVEETDIVSALQNQLAELENLFAALA
                     EEKGAHAYAEGKWSIKELIGHLIDGERVFAYRAFRFSRADQTALAGFEQDDYITYGNF
                     NNRPLADLIEEFSLLRKANLHLFKN"
     gene            446..859
                     /locus_tag="VGB68_01550"
     CDS             446..859
                     /locus_tag="VGB68_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047940.1"
                     /translation="MKSKTIIAIAVLLFCCANIFAQNGGKAEPNRIKFAAGKSSAIVS
                     GTVKGDEQAEYVFSAKEGQMIYLKITSAPKGKAASFRILNPDAEAKFYTKYSVNYEYA
                     FPAPYTGDYVIWVSLQPETKAKSAKYNLALKITKP"
     gene            complement(849..2027)
                     /locus_tag="VGB68_01555"
     CDS             complement(849..2027)
                     /locus_tag="VGB68_01555"
                     /inference="COORDINATES: protein motif:HMM:NF025059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY0047941.1"
                     /translation="MAIENVEKFIGELARSLQEETFVKLTLGNYKGADAHLQKILVRL
                     VETKRGTRLFFLFRYDTRDTAKNFDFNEGVAKVRASLGEDFFAGHLFTTENDFQIDIG
                     KKEKSRLNTAKPTFKTKPSVAHDKEKQILVDPDSFYLRALGITTDKGEIRDKQHDKWK
                     QINKFVETLSKFFANSLLKDSRALKIVDMGSGKGYLTFATYDYFKNNLNLDVQVTGVD
                     TKKELVSLCNDIAKSCDFNDLQFVNGWIGDYDLENADILIALHACNTATDDAIFKGIR
                     ASADLIVCSPCCHQEIRPQIKPPALLRDILKHGVMLEREAEIITDGLRAMLLEKSGYA
                     TRVFEFIATEHTPKNNMIVGTKQNARVDAEKYEREIADIKNFYGITEQRLESLLKTMA
                     "
     gene            2083..2520
                     /locus_tag="VGB68_01560"
     CDS             2083..2520
                     /locus_tag="VGB68_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047942.1"
                     /translation="MKSVLYLLRETFEGSPEGEGSAFLDRGVSFFNTLEKLSAEQVSR
                     EFGATTIAAQTEHAKFYLDRLCEFMTGRTERVNWEQSWLIEEVNEAEWDALRASVRKS
                     YESTLRCFAGIEDWSEDNVGMAIGIVAHTAYHLGAIRQLAKLA"
     gene            complement(2517..2900)
                     /locus_tag="VGB68_01565"
     CDS             complement(2517..2900)
                     /locus_tag="VGB68_01565"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0047943.1"
                     /translation="MDKSFTSYELDRIQPQYNQALFLRDTGRVKDAIKILKNLNKQYN
                     DKPALIGTLGSLYFNAKDWKNCSEYISQGLKLQPQSEYLSLMLFHSLWHMKKFDDALE
                     EVKRFIALNGYSKEYDLILKEIEGD"
BASE COUNT          735 a          692 c          769 g          796 t
ORIGIN      
        1 atttttaaac agatgcaagt tcgcctttcg caaaagcgaa aattcctcga ttaaatcggc
       61 gagcgggcga ttattaaaat ttccgtaagt gatataatcg tcctgttcga aaccggcgag
      121 cgccgtttgg tcggcgcggc tgaagcgaaa ggctcggtat gcgaaaaccc gctcgccgtc
      181 aatcaaatga ccgattaatt ccttgatgct ccatttgccc tcggcgtatg cgtgagcgcc
      241 tttttcttcc gcgagcgcgg cgaaaagatt ttcgagttcg gcaagctgat tttgcaatgc
      301 ggaaacaatg tcggtctctt cgaccaggga cacgtatgtt tcgtaatatt cggcgtactc
      361 gtttttttcg ggtcttgaca taatttcgat tatattagca aaaacggcgt gaaattgcgt
      421 tcataccggc ggtaacgaaa atattatgaa atcaaaaaca attattgcaa ttgcggtttt
      481 acttttctgc tgcgctaata ttttcgcgca gaacggcgga aaagccgaac cgaatcgcat
      541 aaaattcgcc gccggaaaat cttcggcgat tgtcagcggc acggtgaaag gcgacgagca
      601 agccgaatac gttttttcgg cgaaagaagg acagatgatt tatctgaaaa tcacgtccgc
      661 tccgaaggga aaagccgcgt cgttcagaat tttaaacccg gacgcggaag cgaagtttta
      721 cacaaaatac agcgttaatt atgaatacgc ttttcccgcg ccctatacgg gcgattatgt
      781 tatctgggtc agcctgcagc cggaaacaaa agcgaaatcg gcgaaatata atctggcttt
      841 gaagataact aagccatagt tttaagcagc gattcgagcc tttgctccgt gatgccgtaa
      901 aagtttttga tgtcggcgat ttcgcgctcg tatttttcgg cgtcgacgcg cgcgttctgc
      961 tttgtgccga caatcatatt gtttttcggc gtgtgctcgg tggcgataaa ctcgaaaacc
     1021 cgggtcgcgt agccgctttt ttccagcagc atcgcgcgca gaccgtcggt gatgatttcg
     1081 gcttcgcgtt cgagcatcac gccgtgtttc agaatatctc tcaaaagcgc gggcggcttt
     1141 atctgcgggc gaatttcctg gtgacagcac ggcgagcaga cgattaaatc ggcgctcgcc
     1201 ctgatgcctt tgaaaatcgc gtcgtcggtc gccgtattgc aggcgtgaag cgcgatgaga
     1261 atgtccgcgt tttccaaatc gtaatcgcca atccagccgt tgacgaactg caaatcatta
     1321 aaatcacagg acttggcgat atcgttgcag agcgagacga gctctttttt ggtatcgacg
     1381 cccgtgacct gcacgtcgag attcagattg tttttgaaat aatcgtaagt cgcaaaagtc
     1441 agatatcctt tgcccgaacc catatcgacg attttaagag cgcggctgtc tttcagaagc
     1501 gaattggcga aaaacttcga taaagtttcg acgaatttat ttatctgttt ccatttatcg
     1561 tgctgcttgt cgcggatttc gcctttgtcg gtcgtgatgc cgagcgcgcg caggtaaaag
     1621 ctgtccgggt caaccagaat ttgtttttct ttatcgtggg cgaccgacgg ctttgtctta
     1681 aaggtcggtt ttgccgtgtt caggcgcgat ttttcttttt tgccgatgtc gatttgaaaa
     1741 tcgttctcgg tcgtgaaaag atgaccggcg aaaaaatctt cgcccaaact cgcgcgcact
     1801 ttggcgacgc cttcgttgaa atcgaaattt tttgccgtgt cgcgcgtatc gtagcggaaa
     1861 aggaaaaaaa gccgcgtgcc ccgcttggtt tcgaccagac ggacgagaat cttttgcaga
     1921 tgcgcgtccg cgcctttata attacctaaa gtcagcttga cgaaagtttc ttcctggagc
     1981 gagcgagcca gctcgccgat aaatttttcg acgttttcga ttgccataaa tcggagacca
     2041 cagattacac aaatgaacac cgatattgca aaagacgctt ttttgaaatc agttttatat
     2101 ctgctgcgcg agacgttcga aggctcgccc gaaggcgagg gcagcgcttt cttagaccgc
     2161 ggcgtgagtt ttttcaacac gctcgaaaaa ctttccgccg agcaggtttc gcgcgagttc
     2221 ggcgcgacga cgattgcggc gcagaccgag cacgcgaaat tttatctcga ccgtttgtgc
     2281 gagtttatga cgggcagaac cgagcgcgtc aactgggaac aaagctggtt gattgaagag
     2341 gtcaacgagg cggaatggga cgccctgcgc gcatccgtcc gcaaatctta cgaaagcacg
     2401 ctgcgctgtt ttgccggaat cgaggattgg agcgaggata atgtcggaat ggcgattgga
     2461 atagtcgcgc acacggcgta tcatctcggc gcgatccggc agcttgccaa gctcgcttaa
     2521 tcgccttcga tttctttaag aatcaaatcg tattctttgg aatagccgtt gagcgcgata
     2581 aaacgcttta cttcttcgag cgcgtcgtcg aattttttca tatgccagag cgaatgaaac
     2641 agcatcagcg aaagatattc cgactgcggt tgtaacttta gtccctgcga gatgtattcc
     2701 gagcagtttt tccaatcttt ggcattgaaa tacaagctgc ccagagttcc gatgagcgcc
     2761 ggtttatcgt tatactgctt gttcagattt ttcaggattt tgatggcgtc cttgactctg
     2821 ccggtatcgc gcaaaaaaag cgcctggtta tactgaggct gaatgcggtc tagttcatag
     2881 ctggtaaatg atttgtccat catccgagta tagcagagtt ttgaaaatat agagccttga
     2941 taaagcgaaa atgcagttta cgaaaaggcg aaaggcgcgt tggagaaaat cg
//
LOCUS       DASWNE010000075         3566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_73633, whole genome shotgun sequence.
ACCESSION   DASWNE010000075 DASWNE010000000
VERSION     DASWNE010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73633"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(283..1224)
                     /locus_tag="VGB68_01570"
     CDS             complement(283..1224)
                     /locus_tag="VGB68_01570"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0047944.1"
                     /translation="MLRYMLLRIGGLIGVLLIVSALTFGLMHTVPGGPFDARALEKQQ
                     TIPENIKRRLNEKYGLDEPVWKQYALFMRNAATLDFGYSMTYPSRTVVGIFKDQWIYS
                     LELGFLTFVFSIVVGVGLGILSAIKPNSFFDYFGTGVSIFCLVMPSFVFAILLQFVFS
                     AWLGWLPTGGWSGARHWIMPVLANSLAPILVLQRYTRASMSDAIKANYVRTARAKGLS
                     RRRIMFVHVFKNALTPLLTVGGPLAAGLIVGSFFVESIFRIPGIGFYFVSAIGSRDYP
                     MIMATTLVWTLVISLAYLITDLLYALADPRVTLLKEE"
     gene            complement(1318..1827)
                     /locus_tag="VGB68_01575"
     CDS             complement(1318..1827)
                     /locus_tag="VGB68_01575"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0047945.1"
                     /translation="MKIIETERLLLRQATEDDAEFIFELVNEPGWIQNIGDRGVRTID
                     DARRYTANNIVAGYERFGFGFYLVELKESGASAGICGLVKRDSLEDVDIGFAFLERFW
                     SRGFAFESASAVVDYAQNSLGLKRILAIVSPGNRGSIKVLEKIGLRFERMIKMPADDE
                     EIKLFARDF"
     gene            complement(1835..2407)
                     /locus_tag="VGB68_01580"
     CDS             complement(1835..2407)
                     /locus_tag="VGB68_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0047946.1"
                     /translation="MTFKIVEETSDILSEYEKVPISFSVESYFRVELRENGLGGIKLI
                     EEPVVPYLKDYDADENYRPSGWLRRFDISNWGILSAFDGKRRIGGAGIAWNTPEVDML
                     EGWRDLACLWDLRVHPDYRGRGVGHRLFAYTVKWSRARKCRRLMVETQNINVPACRFY
                     ARQGCELGAVNRFAYDENLNETQLLWYRNV"
     gene            complement(2567..3532)
                     /locus_tag="VGB68_01585"
     CDS             complement(2567..3532)
                     /locus_tag="VGB68_01585"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0047947.1"
                     /translation="MSETLLQTTELSKSFAARRGVFGKKFENIAVDRVNLSLLEGETL
                     GLVGESGCGKSTLALTIMRLYEANGGRILFDGKDITHADERALKPIRKQMQMIFQNPL
                     ASLDPRLCVEQIIGEPLEIHGIGTKNEITAEVERLLERVGLPAEAMHRFPSEFSGGQQ
                     QRIGIARALALRPKILICDEAVSALDVSVQAQILNLLRDLQDAFCLAYLFISHNIAVT
                     AFMSRRIGVMYLGRLVEIGAAQQVTRAPRHPYTQTLISAIPEPDPSHRAVEANVKGEI
                     DAVAERPTGCVFRLRCPLAQAICTTEEPQLRVIAENQEVACHFAL"
BASE COUNT          890 a          958 c          896 g          822 t
ORIGIN      
        1 cgcggcgacg gcgacgatta tctgaaaaat aataaaacaa aaggcgacga cggcgatttt
       61 gttgcgggca aaacgccgcc agaaacgcag aagaccgctt gcgcggcgat tgattgagat
      121 tttgtttatt gctgctgcgc ctgtcgccat tgtaaaaaat caggaaattt tgaaaccaca
      181 gatttgcacg gataaacgcg gatagcttga cagggtagag cgggataaaa ttttttctct
      241 tatctgagct aatccgtgtc aatccgcggt ttcaaaattc ctctattctt ccttcagcaa
      301 agtcacgcgc gggtcagcca gcgcgtagag caaatcggtt attaaatacg ccagactgat
      361 aacgagcgtc cagacgagcg tggttgccat aatcatcggg taatcgcggc tgccgattgc
      421 cgaaacgaaa taaaaaccga tgcccggaat acgaaagatg ctttcgacga aaaacgagcc
      481 gacgattaag ccggcggcga gcggtccgcc gacggtcagc agcggcgtca gcgcgttttt
      541 gaaaacgtgg acgaacataa tccgccggcg gctcaagcct ttggcgcgtg cggtgcggac
      601 gtaattcgcc tttatcgcgt ccgacatact ggcgcgcgtg taacgctgca aaaccagaat
      661 cggagccagc gaattggcaa gcaccggcat aatccaatgg cgcgcgcccg accagccgcc
      721 ggtcggcagc catccgagcc acgccgaaaa aacgaactga agaagaatcg caaaaacaaa
      781 gctcggcatc accagacaga aaatcgatac gcccgtgccg aaataatcaa aaaatgagtt
      841 cggtttaatt gccgacagga tgcccagccc gacgccgacg acaattgaaa aaacaaaagt
      901 taaaaatccg agctccagcg agtaaatcca ttggtctttg aaaattccga cgaccgtccg
      961 gctcgggtaa gtcatcgaat aaccgaaatc cagcgtcgcg gcgttgcgca taaacagcgc
     1021 gtattgcttc cagacgggtt cgtccaagcc gtatttttcg ttcagccggc gtttgatgtt
     1081 ttcgggaatc gtctgctgtt tttcgagcgc gcgggcgtcg aaaggtccgc ccggaaccgt
     1141 gtgcatcagc ccgaaggtta aagcggaaac aatcagcaaa acgccgataa gcccgccgat
     1201 tctcaacaac atataacgca gcatatattt attaaggttt tgaattttga gtcttgaatt
     1261 ttgagttttc ggcgcgggcg aaccgtttaa aactcaaaat tcaagactcg aaacttttca
     1321 gaaatcacgc gcaaacaatt tgatttcttc atcgtcggcg ggcattttaa tcattctctc
     1381 gaatcgcaag ccaatctttt cgagcacttt tatcgagcct cgattgcccg gcgaaactat
     1441 cgccagaatg cgctttagtc cgagcgagtt ttgcgcgtaa tccaccacgg cggacgccga
     1501 ttcaaaggcg aagcctcttg accagaatct ctctaaaaag gcaaagccga tatcgacgtc
     1561 ttccagcgaa tcgcgcttga ccagcccgca aattccggcg gacgcgcccg attctttcaa
     1621 ttcgaccaga tagaagccga aaccgaatcg ctcgtaaccg gcgactatat tattggcggt
     1681 atatcggcgc gcgtcgtcaa tcgttctgac gccgcggtcg ccgatatttt gtatccagcc
     1741 cggctcgttg acgagctcga aaataaactc cgcgtcgtct tcggttgcct gccgcagtaa
     1801 taatcgttcc gtttcaatga ttttcatttt cgagttagac atttcgatac cagagcagtt
     1861 gagtctcgtt caaattttcg tcgtaagcaa acctgtttac cgctccgagc tcgcagccct
     1921 gccgcgcgta aaaacggcac gccggaacgt taatgttctg cgtctcgacc atcaggcgtc
     1981 ggcattttcg cgcccgcgac catttcacgg tataggcgaa caaccggtgt ccgacgcctc
     2041 gcccgcgata atccggatga acgcgcaaat cccacaggca ggctaaatct ctccagcctt
     2101 cgagcatatc gacttccggc gtattccacg ctatccctgc gccgccgatg cgacgctttc
     2161 cgtcaaaagc cgataaaatt ccccaattgg aaatatcaaa acgccgcagc cagccggaag
     2221 gtctgtagtt ttcatccgca tcgtaatctt taaggtatgg aacgaccggt tcttcgataa
     2281 gctttattcc gcccagaccg ttttcgcgca attcgacgcg aaaatacgac tcgacgctga
     2341 atgaaatcgg cactttttcg tattcggaca aaatgtcgga cgtttcttcg acgattttaa
     2401 aggtcataac tcgttagccc tttcaaaaga tttttacctt tgccgtgtgt ttttaataac
     2461 gcgcctgacg aaagaattgt tcgatggcgg aattatacga aattaatcct gtttttacgc
     2521 tttgagaaat aatttttacg tcctcaaaaa tctctctctt tgatttctaa agcgcgaaat
     2581 gacaggcgac ttcctgattt tcggcaatca cgcgcagttg cggttcttca gtcgtgcaaa
     2641 tcgcctgcgc gagcgggcag cgcagacgga aaacgcagcc cgtcgggcgc tccgcgacgg
     2701 cgtcaatctc gcccttcacg tttgcttcga ccgcgcggtg cgacgggtcg ggttcgggaa
     2761 tcgcggaaat caaagtttgc gtatacggat gacgcggcgc gcgcgtcact tgctgcgccg
     2821 cgccgatttc cactaatctt cccagataca ttacgccgat gcgacggctc ataaaagccg
     2881 tcaccgcgat attgtgcgag ataaaaagat aagccaaaca gaatgcgtcc tgcaaatcgc
     2941 gcaggagatt caggatttgc gcctgcacgg aaacgtccag cgccgagacc gcttcgtcgc
     3001 aaattaaaat cttcgggcgc agcgcgagcg cgcgggcaat cccgatgcgc tgctgctgcc
     3061 cgccggaaaa ctcggacgga aaacggtgca tagcttcggc gggcaagccg acgcgctcga
     3121 gcaggcgctc gacttcggcg gttatttcgt ttttcgttcc gatgccgtga atttccaggg
     3181 gctcgccgat gatttgttcg acgcagagac gcgggtcgag cgaggcaagc gggttctgaa
     3241 aaatcatctg catttgtttg cgaattggct ttaacgctcg ctcgtcggcg tgagttatat
     3301 cttttccgtc aaacagaata cgcccgccgt ttgcctcgta aagacgcata atcgttaagg
     3361 cgagagtcga ttttccgcag ccggattcgc cgaccagccc gagcgtctcg ccttcgagca
     3421 gagacaaatt tacgcggtcg accgcgatat tctcaaactt tttgccgaag acgccgcgcc
     3481 gcgccgcgaa gcttttgctt aattcagttg tttgcagcaa tgtttcggac atttttaata
     3541 agtaagcagc gagagctgag aattga
//
LOCUS       DASWNE010000076         2430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_249997, whole genome shotgun sequence.
ACCESSION   DASWNE010000076 DASWNE010000000
VERSION     DASWNE010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249997"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VGB68_01590"
     CDS             <1..528
                     /locus_tag="VGB68_01590"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0047948.1"
                     /translation="FSMNRFEDETFDAVLADASFVRPDDLQKFLEEAARVTETGGKVA
                     VFTVTAGSFGEIFSFLWEIFFNNGLGDHGVAAEKLITEIPTVWRVEEMAQAAGLKNVV
                     SETENQIFEYENGAEFVNSTLVGDFLLPIWLKFLNGKEKAQVSKKLARLVDEEDHDLS
                     FRFSVKATLVTGEKA"
     gene            complement(611..913)
                     /locus_tag="VGB68_01595"
     CDS             complement(611..913)
                     /locus_tag="VGB68_01595"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEY0047949.1"
                     /translation="MLGLGTYEILIIVAILFLLFGATRLPQLAKSLGQTRKAFKDGMR
                     EAEEEERLEQEQKRLQDSRNVATLNSPSVASPTVTELDDETLFEEARRRATQAKQQ"
     gene            complement(1024..1980)
                     /gene="pip"
                     /locus_tag="VGB68_01600"
     CDS             complement(1024..1980)
                     /gene="pip"
                     /locus_tag="VGB68_01600"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEY0047950.1"
                     /translation="MKSLYPEIEPFDSGMLKVSEIHEIYYERVGNPKGIPVVFLHGGP
                     GGGLIPLYRQFFDPQAFHVVLFDQRGSGQSTPHAELNENTTWDLIRDIESLREKFGVE
                     KWYVFGGSWGSTLSLAYAETHPDRCLGLILRGIFLTRRKELLWFYQYGASEIFPDFWQ
                     RYRDEIPFEERGDFMTAYYKRLTSDDEATRLSAARAWSVWEGSTSKLYPDKDLMEHWE
                     GAHEALSLARIECHYFMNNSFFPSENYLIENVDKIRHIPTVIVQGRYDVVCPMTSAWE
                     LHRAFPEAEFIVVPDAGHSVSEKGITSALVGAMDRFKEKGQS"
BASE COUNT          591 a          596 c          596 g          647 t
ORIGIN      
        1 ttttcgatga atcgttttga agacgaaact ttcgacgcgg ttttagccga cgccagtttc
       61 gtgcgtcccg acgatttgca aaaatttctc gaagaagccg cgcgcgtcac cgaaaccggc
      121 ggcaaagtcg ccgtgtttac cgtcacggcg ggcagtttcg gcgagatttt ttcctttctc
      181 tgggaaatat tttttaataa tggtttaggc gaccacggcg tcgcggcgga aaaactcatt
      241 accgagatac cgaccgtctg gcgggtcgaa gaaatggcgc aggcggcggg cttgaaaaac
      301 gtcgtgtcgg agacggaaaa ccagattttc gaatacgaaa acggcgcgga atttgtcaat
      361 tccacgctgg tcggcgattt tcttctgcca atctggttaa agtttttgaa tggaaaggaa
      421 aaagcgcagg tttcaaaaaa actcgcgcga ttagtcgatg aagaagacca cgatttaagt
      481 ttccgctttt ccgtgaaagc gacgctcgtc acgggcgaaa aagcttaaag gtgaaaaaaa
      541 gaaaaggcgg aaaagtgaaa aggtaagtcg agtctattca acctgctttt tcacttttcg
      601 ccttttcttt ttactgctgc tttgcttgcg tggcgcggcg acgggcttct tcaaaaagcg
      661 tttcgtcgtc cagttccgtg acggtcggtg aagcgacgct cggtgaattt aaagtcgcca
      721 cgttgcgcga atcctgaagg cgtttttgtt cctgttccag gcgttcttcc tcttcggctt
      781 cgcgcattcc gtctttaaac gctttgcgcg tttgtccgag cgatttcgcc agctgcggca
      841 ggcgcgtcgc accgaatagt aaaaacaaaa tcgctacaat tataagtatt tcgtaagttc
      901 ctaatccaag cataaatttc taccaatcct ttggacgcgc aaatggtcgc agaagtcggc
      961 gcgccttttt ttcatcatag cgctatttgc ttaatgttgc cattacaaaa gcgtttctgc
     1021 cttttaactt tgaccttttt ccttaaatct gtccatcgcc ccgaccagcg ccgacgtgat
     1081 tcctttttcc gaaaccgagt gtccggcgtc cggcacgacg ataaactcgg cttcgggaaa
     1141 agcccggtgc agctcccacg ccgaagtcat cgggcacacc acgtcataac gtccctgcac
     1201 aatgacggtc ggaatgtggc ggattttgtc tacgttctcg atcagataat tttccgaagg
     1261 gaaaaacgaa ttgttcataa agtaatgaca ttcgattctc gccaggctga gcgcttcgtg
     1321 cgcgccttcc caatgctcca tcaaatcttt atcgggatag agcttcgagg tcgagccttc
     1381 ccagacgctc cacgcgcggg cggcggaaag gcgcgtcgct tcgtcgtcgc tggtcaggcg
     1441 cttgtaatac gccgtcataa aatcgccgcg ctcctcaaac ggaatttcgt cgcgatagcg
     1501 ttgccagaaa tcgggaaaaa tctcgctcgc gccgtattga taaaaccaca ataattcctt
     1561 gcgccgggtc agaaaaattc cgcgcaggat taagcccaga cagcggtcgg gatgcgtttc
     1621 ggcgtaagcc agactgagcg tgctgcccca cgagccgccg aacacatacc atttttcgac
     1681 gccgaatttt tcgcgcagcg attcgatgtc gcgaatcaaa tcccaggtcg tgttttcatt
     1741 aagctcggcg tgcggcgtcg attgccccga gccgcgctgg tcgaacaaaa cgacgtgaaa
     1801 cgcctgcggg tcaaaaaact gtcgatagag cggaatcaat ccgccgcccg gtccgccgtg
     1861 cagaaagacc acgggaatcc ctttgggatt gccgacgcgc tcgtaataaa tttcgtggat
     1921 ttcggaaact ttcagcattc ccgaatcaaa cggttcgatt tccggataga gacttttcat
     1981 aattctctga ataatacaaa atcccgccga aaaaaactat tgccgcgcat cgacgctttt
     2041 atggaatgcg aaaagtttta ggcttttctt tatagaaata gatttactcc cgaaagcgaa
     2101 aaccagagat tccgtgttcg gtggaagcac ggaataaaga gggagcatta gctttttgaa
     2161 gaaaagtcgg ctaatcgctc ccctttttgc gtccggctcg tattttgtcg gcaataaatc
     2221 tcccgaaatc cgtctatgta aaaccggcgg ctttttcgtt tgccgttgcg ttttaaattt
     2281 tgacggctcg ctgccctgat aagaatttac tttaactgtt tgtatttctg gcattgtatt
     2341 tgcctaatca ggaaagcgga attatttatt gatagtttat gttttgattt taaattttat
     2401 tcgcagctaa taggggtttt atggctaaga
//
LOCUS       DASWNE010000077         3087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_496788, whole genome shotgun sequence.
ACCESSION   DASWNE010000077 DASWNE010000000
VERSION     DASWNE010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496788"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2811
                     /locus_tag="VGB68_01605"
     CDS             <1..2811
                     /locus_tag="VGB68_01605"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEY0047951.1"
                     /translation="SSLAQAQDAPPPSPSPTPITAQNFHQWGAVTLFNGLPSDNARAI
                     AQTPDGVLWFGTDNGLTRFDGRRVQTVAPENIESSKIFALEIDGDGRLWVGTERGAYF
                     LQNDIFKKIEETADFAITSILPRENIYFSTADGVVFRLTANNENSFSVEKIPAEPLVG
                     SDGKLLKITGLIEKDGTLVAATRSRGILVLEDTEFFETFSRPRPFFVNALAQDESGGA
                     AWLGADAKSGESGFFSLRDIARPEKIGSDLGNVLAIEADAGGVWVGTQAKGLFYFRGA
                     QQMKNFTFENTAGGLRSNTIYALFVDREGVLWIGTNRGVSRFDASSPFTRILSAENSN
                     GNFVRALYQTKDRRIFAGTNRGLFEFDGANWLEIPNFAARAVYTISENSLNQTLLAAP
                     TGIFSLDGKQILAGDARSTVEFQGKTYAAVFGRGILQIDGANQTQIFANDAPTALYAD
                     GEKLWIGTAKGEVFTFDGKQMRQEKALEALRETALRKIAKDAEGSLWFGGANGLFRFK
                     NGELQNVVAGREVRDFLTDGAEIWAATLNGGLVHVKADENFGWIAADINVEQGLPSEQ
                     IFALLRQENQLLIGTNRGGVAYAPSAIPPQIIAARILSQRLHNAEEAAQTIKLEYPQN
                     SILVEVAGLSSRTFPEQFQYAFFLKNSKGETLEKKLSSDSQFAPANLDAGEYRIEARA
                     FNKDLLASEPLIIRFSIDREPFPWTTTALGVLLAIALIALIWAIVERKRISNRNRELA
                     AARFDLANEAERERKRIARDLHDQTLADLRNLMLKTDKLPADNSDFRAEIESVSTEIR
                     RICEDLSPSVLENVGLTAALEFLLSHTIENYKFSAAENLEESLNFSSNAQMQIYRIAQ
                     EILSNIKRHSDADAVEMKIEISAENEFILSISDNGTPFAPAADTARGRGISNVKSRAA
                     LIKAEVFWRTNENGNTLFELRK"
BASE COUNT          756 a          852 c          807 g          672 t
ORIGIN      
        1 tcatcattag cgcaggcgca ggacgcgccg ccgccgtcac cttcgccgac gccgataacc
       61 gcgcagaatt ttcatcaatg gggcgcggtc acgcttttta acggcttgcc gtcggacaac
      121 gcgcgcgcca tcgcgcaaac gcccgacggc gttttgtggt tcggcacgga caacggcttg
      181 accagatttg acggtcgccg cgtgcagacg gtcgcgcccg aaaatatcga atcgagcaaa
      241 atcttcgcgc tcgaaatcga cggcgacgga aggctctggg tcggaaccga gcgcggcgcg
      301 tattttctgc aaaacgatat ttttaagaaa atcgaagaaa cagcggattt tgcgataacc
      361 tcgattctgc cgcgcgaaaa catttatttc tcgaccgccg acggcgtcgt tttcagattg
      421 accgccaata acgaaaattc attttccgtc gaaaaaatac ccgctgagcc gctcgtcggc
      481 agcgacggca agcttctgaa aatcaccggt ttgattgaaa aagacggaac gctcgtcgcc
      541 gccacgcgca gccgcggcat tttagttctg gaagacacgg aattttttga aaccttcagc
      601 cgcccgcgcc cgttttttgt caacgctctg gcgcaggacg aaagcggcgg cgcggcgtgg
      661 ctcggcgcag acgcgaaaag cggcgagagc ggattttttt cgcttcgaga catcgcgcgt
      721 cccgaaaaaa tcggctctga cctcggcaat gttctggcaa tcgaagcgga cgcgggcggc
      781 gtctgggtcg gaacgcaggc gaaaggcttg ttttattttc gcggcgcgca gcagatgaaa
      841 aatttcacgt ttgaaaatac ggctggaggc ttgcgctcga acacgattta cgcgcttttt
      901 gtcgaccgcg aaggcgttct ctggattggg acaaatcgcg gcgtcagccg tttcgacgcg
      961 tcgagtcctt tcacgcggat actttccgcc gaaaactcga acggaaattt cgtgcgcgcg
     1021 ctttaccaga caaaagacag gcgaatcttt gccggaacaa atcgcggatt attcgaattt
     1081 gacggcgcaa actggcttga aattccgaat tttgccgctc gcgccgttta tacgatttcc
     1141 gaaaattcgc tgaatcagac gcttttagcc gcgccgaccg gcattttttc gctggacgga
     1201 aaacaaattc tcgccgggga tgcgcgctcg accgtcgagt ttcagggaaa aacctacgcc
     1261 gccgttttcg gacgcggaat ccttcaaatc gacggcgcga atcaaacgca gattttcgcc
     1321 aacgacgcgc cgaccgcgct ttacgccgac ggcgaaaaac tctggatcgg cacggcgaaa
     1381 ggcgaagttt tcaccttcga cggcaaacag atgagacagg aaaaagcgct cgaagctctg
     1441 cgcgagacgg ctctgcgaaa aattgccaaa gacgccgaag gcagtttatg gtttggcggc
     1501 gcaaacgggc ttttccggtt taaaaacggc gagctgcaaa acgttgttgc gggtcgcgaa
     1561 gtgcgcgatt ttttgacgga cggcgcggaa atctgggcgg cgactttaaa cggcggactc
     1621 gtccacgtca aagcggacga aaatttcggc tggattgcgg ctgatataaa tgtcgaacag
     1681 ggcttgcctt cggaacaaat tttcgccttg ctgcgacagg aaaatcaact gcttatcggc
     1741 accaatcgcg gcggcgtcgc ctacgcgccg agcgcgattc cgccgcagat aatcgccgcc
     1801 cgcatcttga gccagcgtct tcacaacgcg gaagaagcgg cgcagacgat taagctcgaa
     1861 tacccgcaaa actctatttt agttgaagtt gcgggcttga gcagccgcac ctttcccgaa
     1921 cagtttcaat acgcgttttt tctgaaaaac tcgaagggcg aaacgctgga gaaaaaattg
     1981 tccagcgact cacagtttgc gcccgccaat ctcgacgccg gagaataccg aatcgaagcc
     2041 cgcgctttta ataaggattt gctcgcctcc gagcctctga ttatcagatt ttcgattgac
     2101 cgcgaacctt ttccctggac gacgaccgcg ctcggcgtgc tgctggctat cgctttaatc
     2161 gctttgattt gggcaattgt cgagcgaaaa agaatcagca accgcaaccg cgagcttgcc
     2221 gccgcgcgtt ttgatttggc gaacgaagcc gagcgcgaaa gaaaaagaat cgcccgcgac
     2281 cttcacgacc agacgctcgc cgatttgcga aacctgatgc tgaaaaccga caagcttccg
     2341 gcggacaatt ccgattttcg cgccgaaatc gaatcggtct cgaccgagat tcggcgcatc
     2401 tgcgaagatt taagcccgtc cgtgctggaa aacgtcgggc tgaccgccgc gctcgaattt
     2461 cttttgagcc acacgattga aaactacaag ttttccgccg ccgaaaatct ggaagaaagt
     2521 ctgaattttt cgtccaacgc gcagatgcag atttatcgca tcgcgcagga aattttgagc
     2581 aacatcaaac gccactccga cgccgacgcc gtcgagatga aaatcgaaat ctccgccgaa
     2641 aacgaattta ttctctccat ctccgataac ggaacgccgt ttgcgccagc cgccgacacc
     2701 gcgcgcgggc gcggcatttc caacgtcaaa tcccgcgccg cgctgattaa agccgaagtc
     2761 ttctggcgca caaacgaaaa cggcaacacg cttttcgagc tgcgcaagta aaagcggtga
     2821 gcggtaagca gtgagcagta ttagattcaa ttcctcaatg gtaaatttca aaaaactact
     2881 gcttaccgct cactgctcac tacatcgaac tgtggaaatc cacattcggc aaacagcgta
     2941 agccttccta attcccgttg attagcccgt tcaaaccgaa ctttcaataa tcgataatcg
     3001 ctctcaagtt atgcgattac cgtttaaaaa aatcttttat ctggcgcttt tggcgctggt
     3061 tttaagcttt gtcatttttc atatttg
//
LOCUS       DASWNE010000078         1815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_179118, whole genome shotgun sequence.
ACCESSION   DASWNE010000078 DASWNE010000000
VERSION     DASWNE010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179118"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VGB68_01610"
     CDS             complement(<1..516)
                     /locus_tag="VGB68_01610"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047952.1"
                     /translation="MTQIEDIKIFLKDLPDADSAERFFRQLSEQKPSEANKILKNKGL
                     LSDVLTLVSFSPLLATTLLQNPQYISWLARRRSEAKMRDKEELLESLARFSMTNSQIE
                     PPVLLARFRRRELLRIYLRDIRRLGTIAEITEEISHLADAVLEYALRLARQEMDNRYG
                     IPLESDEKGKAR"
     gene            complement(683..>1815)
                     /locus_tag="VGB68_01615"
     CDS             complement(683..>1815)
                     /locus_tag="VGB68_01615"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY0047953.1"
                     /translation="LGVEVVYDPENDFESLFNFLAVIAAMISQALKSQTEIATEKEKL
                     FEETSHLQQELREKYDFNNLIGNSGPMRQIYAQVSQVARSNATVLLRGESGTGKEMIA
                     NAIHYNSLRAKKPIIKINCAALPETLIEAELFGYEKGAFTGANQRKKGRFELADGGTL
                     FLDEIGDLPLQTQVKLLRVLQEREFERLGGTETLKVNVRLITATNKNLEAEIAGGNFR
                     EDLFYRLNVFTIFLPPLRERKSDILLLSEHFLEKYEKIHGKVVKRISTPAIDMLTAYH
                     YPGNVRELENAIERAVVVCDGNVIHGHHLPPTLQTAEVSDTVTKATLESAVNAFERDL
                     IQDTLKTTNGNCAKAANMLNTTERIINYKIKKYGIKPKRFRA"
BASE COUNT          420 a          462 c          458 g          475 t
ORIGIN      
        1 ccgcgccttt cctttttcgt cggattccag cgggatgccg tagcgattgt ccatttcctg
       61 tcgcgccagg cgcagcgcgt attccagaac ggcgtccgcc agatgcgaga tttcctccgt
      121 gatttcggca atagtaccga gacggcgaat atcgcgcaga taaattcgca gcagctcgcg
      181 gcggcgaaaa cgcgccagca aaaccggcgg ctcaatctgc gaattggtca tcgaaaaacg
      241 cgccagcgat tcgagcaatt cttctttatc gcgcattttc gcttcggagc gccgccgcgc
      301 cagccacgaa atatattgcg gattctgcaa aagcgtcgtc gccagcagcg gcgagaagga
      361 cactaaagtc aaaacatcgg acaacaaacc tttatttttt aaaattttgt tcgcttcgga
      421 aggcttctgt tcggaaagct ggcgaaaaaa acgctcggcg ctatcggcgt cgggcaaatc
      481 ctttagaaaa attttgatgt cttcgatttg agtcatcggc ggaaaaaacg cgtttcgctt
      541 atgatagcaa aattcaggat ttaggattca ggattcagga tttaagattc aggatttaag
      601 attcaggatt taagattcag gattcagaat tggagattgc ataacggtaa atcctgaatc
      661 ctgaatcctg aatcctgaat ctttacgcgc ggaaacgctt cggcttgatg ccgtattttt
      721 tgattttata attgataatg cgctcggtcg tgttcagcat atttgctgct ttggcgcagt
      781 ttccgttggt ggttttcagc gtgtcctgaa tcaaatcgcg ctcgaacgcg ttgacggcgg
      841 attccagagt cgctttcgtc accgtgtcgg aaacttcggc ggtctgtaaa gtcggcggca
      901 gatgatgacc gtgaatcacg tttccgtcgc agacgacgac ggcgcgctcg atggcgtttt
      961 ccagctcgcg gacgttgccc ggataatgat atgccgtcag catatcaatc gccggcgtcg
     1021 agattctttt cacgactttg ccgtgaattt tttcgtattt ttccagaaaa tgctcggaaa
     1081 gcagcaggat gtcggacttt cgctcgcgca gcggcggcag aaaaatcgtg aaaacgttca
     1141 aacgataaaa caaatcttcg cgaaaattac cgcccgcgat ttcggcttcg agatttttat
     1201 tggtcgccgt aatcagccgc acgttaacct ttaaagtttc cgtcccgccg agccgctcga
     1261 attcgcgctc ctgcaaaacc ctgagcagct tgacctgcgt ttgaagcggc aaatcgccga
     1321 tttcgtcgag aaaaagcgtg ccgccgtccg ccagctcgaa acgtcctttt tttcgctggt
     1381 tcgcgccggt aaacgcgcct ttttcatagc cgaaaagctc ggcttcaatc agggtttcgg
     1441 gcagcgccgc gcagttgatt ttgataatcg gttttttggc gcgcaggctg ttgtaatgaa
     1501 tcgcgttggc aatcatttct ttgcccgttc cgctttcgcc gcgcagcaga acggtcgcgt
     1561 tcgagcgcgc cacctgcgag acctgcgcgt aaatctgccg catcggaccg gaattgccaa
     1621 tcagattatt gaaatcgtat ttctcgcgca attcctgctg caaatgcgac gtttcctcaa
     1681 atagcttttc cttttccgtc gcgatttcgg tctgcgattt taaagcctgc gaaatcattg
     1741 cggcgatgac ggcgagaaaa ttaaataaag attcaaaatc attctcgggg tcgtaaacca
     1801 cctcgacgcc gagaa
//
LOCUS       DASWNE010000079         1749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_214255, whole genome shotgun sequence.
ACCESSION   DASWNE010000079 DASWNE010000000
VERSION     DASWNE010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214255"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VGB68_01620"
     CDS             complement(<1..436)
                     /locus_tag="VGB68_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047954.1"
                     /translation="MSAKILDATRLESLLESAQLLNSSLDLDSLLKHLLRTVMGRTLV
                     GRGFIAVSNNGEMRIAQLRGLRNLKIGDSFDERALRESGITSFYPIGDPDKPTGFLGL
                     GKPPAGEISAEETESLHALLGLAASSIANAQAHDQTRKFNFQL"
     gene            complement(502..942)
                     /locus_tag="VGB68_01625"
     CDS             complement(502..942)
                     /locus_tag="VGB68_01625"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0047955.1"
                     /translation="MKTLERKFTLQVPSSTENLALIREFISSAATQAGFDEKEVAKLE
                     LAVDEACANVIEHAYGHDASKEVIVRARLDDEEFSIDIEDTGKGFDPAEVDQEQLEQL
                     ISKRKTGGLGMRLMKTLMDEVRYEIEPGKKNALHMSKRLKKDKG"
     gene            complement(942..1316)
                     /locus_tag="VGB68_01630"
     CDS             complement(942..1316)
                     /locus_tag="VGB68_01630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0047956.1"
                     /translation="MPNPFSVATDSEDDFAVIRLDGAVDAHTAPQFEEAVQQVIDGGQ
                     NNIVVDCSRLTYISSAGLGVFMGFVEEVREAGGDIKICGLSEKVKQPFEILGFETLFH
                     FCPDVPAAKEKFSEPTDWEAAY"
     gene            complement(1404..>1749)
                     /locus_tag="VGB68_01635"
     CDS             complement(1404..>1749)
                     /locus_tag="VGB68_01635"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEY0047957.1"
                     /translation="AGMPPVLIYRSETKRVEEIGIKALPLGAIAKLAYQKQELQLFEG
                     DVVLLLSDGFPEMFNESGEMLGFEKAAEVLPEIAANEPDEIIRRLVIIGQQWAGSRPP
                     DDDVTFVVLKTV"
BASE COUNT          378 a          468 c          441 g          462 t
ORIGIN      
        1 tgagctgaaa gttgaatttc cgcgtttggt cgtgcgcctg agcgttggcg atagagcttg
       61 ccgccagccc cagaagcgcg tggagcgatt cggtttcttc cgccgagatt tcgcccgccg
      121 gcggctttcc gagccccaag aagccggtcg gtttatccgg gtcgccgatc gggtaaaacg
      181 aagtaattcc gctttcgcgc agcgccctct catcgaacga atcgccgatt ttcaaatttc
      241 ttaagccgcg aagctgcgcg atgcgcattt ccccgttatt ggaaaccgcg ataaatccgc
      301 gcccgacgag cgttctgccc atcacggttc gcagaagatg cttgagcaaa ctgtcgagat
      361 cgagcgacga attgagaagc tgcgccgatt cgagcaatga ttcgagtcgt gtcgcgtcga
      421 gtattttggc tgacataatc ttattgtaag gataaaggat aaaggatgaa ggttaatcgg
      481 aattttcttt tattttaccc tttatccttt atcctttttc aagcgcttgc tcatatgaag
      541 cgcgttcttt ttgcccggtt cgatttcgta acgcacttcg tccatcaggg ttttcatcag
      601 gcgcattccg agaccgccgg ttttgcgctt gctgatcaat tgttcgagct gttcctggtc
      661 gacctccgcc gggtcgaaac ctttgccggt gtcttcaatg tcaatcgaaa attcctcgtc
      721 gtcgagccgc gcgcggacga tgacttcttt gctcgcgtcg tgaccgtagg cgtgttcgat
      781 aacgttggcg caggcttcgt cgacggcgag ctccagcttg gcgacttctt tttcgtcgaa
      841 gccggcttgc gtcgccgcgc tcgagatgaa ttcgcgaatc aacgcgagat tttctgtcga
      901 agacggcacc tgcagcgtaa attttctctc taaagttttc attaatatgc cgcctcccaa
      961 tcggtcggct cgctgaattt ttctttggca gccgggacgt ccgggcaaaa atgaaaaagc
     1021 gtttcgaagc ctaagatttc aaaaggctgt tttacttttt ccgacaatcc gcagattttt
     1081 atatcgccgc ccgcttcgcg cacttcttcg acaaaaccca taaaaacgcc caagccggcg
     1141 gacgaaatat aagtcagccg cgagcagtcg acgacgatgt tgttctgccc gccgtcgatg
     1201 acctgctgga cggcttcttc aaattgcggc gcggtatgcg catcgaccgc gccatcgagc
     1261 cggataacgg cgaaatcgtc ttcggaatcg gtcgcgaccg aaaatggatt tggcataaaa
     1321 aatttttatt ctcctcgaaa aagttaaaat taagtttatt ttgcgaataa agcctctgaa
     1381 ttacaaatcc gcaagccgaa ttttcaaact gttttcaaga cgacgaaagt gacgtcatcg
     1441 tccggcggtc ttgagccagc ccattgctga ccgatgatga ccagccgcct gatgatttcg
     1501 tccggctcgt tggcggcgat ttccggcaaa acttcggcgg ctttttcaaa accgagcatt
     1561 tcgccactct cgttgaacat ttcgggaaaa ccgtcgctca acagcagcac gacatcgcct
     1621 tcgaagagct gtaactcctg tttttgataa gcgagtttcg ctatcgcgcc gagcggcaga
     1681 gccttgatgc cgatttcttc gactcgtttc gtttcgcttc gataaatcaa gacgggcggc
     1741 attccggcg
//
LOCUS       DASWNE010000080        10278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_38357, whole genome shotgun sequence.
ACCESSION   DASWNE010000080 DASWNE010000000
VERSION     DASWNE010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10278
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38357"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VGB68_01640"
     CDS             complement(<1..421)
                     /locus_tag="VGB68_01640"
                     /inference="COORDINATES: protein motif:HMM:NF019931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueG-associated DUF1730 domain-containing
                     protein"
                     /protein_id="HEY0047958.1"
                     /translation="MASFTEKIKQKAFAVGFHKIGVARAEPLAPEAEYLKQWLEKDFH
                     GEMRWMEREPEKRTDPKLIFAEAKSVVVVALNYYTPHRHEQNEPTGKVSRYAWGDDYH
                     DVVKDKLRELLAWIKSENAAVEGKICVDTAPVMDKAWA"
     gene            complement(518..4288)
                     /locus_tag="VGB68_01645"
     CDS             complement(518..4288)
                     /locus_tag="VGB68_01645"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0047959.1"
                     /translation="MRAFSLRFTTVVSRLLALALVTLVFAGAAFAQSQASSADLNGTV
                     TDPSGAVVPGATVTARDTATGITRNTTSDESGEYKFIGLPPSTYEITAEATTFKKTII
                     PSVRLTVGQNAELTIRLEVGAEGATVTVTGGDVELVETTRTTVSSTIDQQRIENLPIN
                     ERSATGFALTISTVGRDNGRPIGPAPTSGLNIGGQRGRSTLVQVDGSDFTDNSINAAR
                     TTVSQEAVQEFQVATNSYAPEFGRATGGIINVVTKRGTNEFTGNVFGFIRDKSIQARN
                     PFAPFKTAFTRTQYGATLGGPLLKDRSFFFFSFERRQRNESGFFTSDVTRDLTSSVTF
                     GGQTFNRLTAEQAQKAQQLIATGNPSAIQAAVGYLYLASGGGTTALTGVNPLVAPPCP
                     TPPATPTNLCNPNPGTPFLIGSRFFLTGAPIPLNSVNNFGEPIAFRPLLGLQPVFPIT
                     DRTNYFSLRGDHYINQNHQLTMRFGYNPSNLSGIQVESQNQSLGQNDFSRTGITKIKD
                     TAFTFGLNSTLGANTANEFRFHFGKRKTSFRSQNNEAVAFNISGTAFIGRELFSPVDR
                     TENRFQLADNLTQVYGDHTFKFGADFNWVEIPSALFELNFAGLFNFGPFAAANLNPSF
                     APLNLPDLTPVQSYGLGLPSLYIQGFGNPISKIKNKPIAFYAQDTWKATRRLTLNYGI
                     RYDVEFTETIAPVAFRDPLTGINLSESDILAAQDALGVQQGIPRDTNNWAPRLGVAYD
                     VFGNGRTVLRGAIGLFYDHPLLAVAFNSDIADAAQQQQAVLTAGSPNPAALLNAAQVF
                     QGTVCGGAAANPLCAPGVITPGLASTAQYQFGRQRFNDQTFTGFGPVLPFTLPIAKDF
                     KYASAVQANLAIEHQLTTNMSLSVGYIFVGARNLPHPTDLNTPNTALQIQNFQRFFGG
                     ALPTTTQQAIAFSIATTPAGTPTGVGCTVLVPGIIAQCPNGRVVAPAVANFFRPNAPN
                     YFLAQSLSSGALTKPVLDSLLAGSLRTPGTLSPFGSVNAQISNGNSEYNALNVELKRR
                     FANNLTFQASYTWAHSIDDSSDLQTLLIAQDVRRFDLEKADSLFDQRHRFVFSGVLAS
                     PAGWRGGNGWQRFLADFTIAPIIEISSGRPFNIITNVDTNNDQSTQTDRPSVNPDGTL
                     CQPGTLGCITPLITNGEFSIGNLGRNMGLTHGYASFDLRVARAIRFGERIRLDLIAEG
                     FNLFNRFNEASASPFLNDVNAVGERSKNGRYFSRSTAAYDPRQFQFGVKLNF"
     gene            complement(4496..5803)
                     /locus_tag="VGB68_01650"
     CDS             complement(4496..5803)
                     /locus_tag="VGB68_01650"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0047960.1"
                     /translation="MIKDKVKIFAGWRQPPNMKKNEREKIVIVGAGPAGTSAAIRLAQ
                     NNFQVTLVEREKFPRPKLCGEFISPECLAHFRELGVIDEMLSAGGERIRKTVFYEPDG
                     KSVAVPSEWFFQGGAGALSLSRAEMDFRLLERAAAVGVEVLEETQALGLLTENEIVGG
                     VKARDRNGEIKEISARLTIDATGRAGILRKLAERGISRRGGVKAQKRKGIWSLKSEAS
                     NYQRPKTKDRSPKLIGFKTHLKNAKIESGVCEIYFFRGGYGGLSRVENGFANHCFLIE
                     SEIVKKAGGDAEKIVREVVFQNKRAEQMMKSAEAGFDWLAVSVDGFGAKDLNPAPRLL
                     AIGDAGAFIDPFTGSGMLMALESGELLARSVAENNFSPEKIAEIYKKRHAEKFRKRLF
                     VCSLMRRAAFAPSLAKSLISVLSRSEKARAILARATRAAALGS"
     gene            6110..6340
                     /locus_tag="VGB68_01655"
     CDS             6110..6340
                     /locus_tag="VGB68_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047961.1"
                     /translation="MKNILKLSRKFIVLGAMLFALGFMTFTNFGTAPADASLFCCSEC
                     EPIYQSCIDNGGTPTQCNQQANYCWRHCDFGC"
     gene            6411..7370
                     /locus_tag="VGB68_01660"
     CDS             6411..7370
                     /locus_tag="VGB68_01660"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0047962.1"
                     /translation="MKIIPLAIPTPFYIGDVNVYLIREEPLTLIDVGPKTKEAAAALR
                     EGLQRNGVRFSDIERIVLTHAHEDHCGLARAVRDEAKNASILVHGWETGHLFGRLARA
                     EHENLLYRAGVPTEIFHKMHDFYEDVRLLTDSLSDGEFDLLHDEMELEFSSGVLKVLH
                     TPGHTPGSCSFIRESNRTLICGDCVLKRITPNPVLSPDPVDARKRFRSLGEYLVSLAR
                     LRSFSPTLTYGGHGEPVTDFEEIFNRYVRAIDERQRNVIDLVTPGGVTAFELALKLFP
                     EAVAENAQRFLAVSEAVAHLDYAESQGKIAVELKEGVEFYRRF"
     gene            complement(7480..8910)
                     /gene="selA"
                     /locus_tag="VGB68_01665"
     CDS             complement(7480..8910)
                     /gene="selA"
                     /locus_tag="VGB68_01665"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEY0047963.1"
                     /translation="MENRETKNHTEALRLLPSIDALLRTQAAQKLISQTGARHLATLA
                     RAVTDLLRRNLQEKIAGGEMLRYSREDLLNEAEKRLTELWQSEQNVGLQRVVNATGVI
                     IHTNLGRAPLSENARKAIFEKAARYCNLEYNLETGKRGRRGARAEHLLAALAGAESAL
                     IVNNCAAAAFLVLTALASGGEVIISRGELVEIGGDFRVPEVLAQSGAVLKEVGATNRT
                     RLSDYENAINEKTRMLLRVHPSNYRIVGFTATPDLKEIAEVAHKNNLILYEDAGSGAL
                     LDLSEYGLGEEPVISRSLVAGADVVTFSGDKLLGGVQAGLIVGRRETVEKLRSHPLYR
                     ALRVDKLIYAALEATFESFRRDTVLQEIPVLKMLSTTSEELKTRTLEFDRKLRERLGE
                     NRRLKFEVAEGKSVVGGGSAPMVQPATTLLALRHEKMTVSRLEEALRLSKPPVIARIL
                     EDKVLIDLRTVSEAEEAELLNVLSKV"
     gene            complement(9030..9176)
                     /locus_tag="VGB68_01670"
     CDS             complement(9030..9176)
                     /locus_tag="VGB68_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047964.1"
                     /translation="MAQNRLKRQPDTPTGATTTMATAWFATATAKVLMRRVRSVPASK
                     ASLQ"
     gene            9306..9626
                     /locus_tag="VGB68_01675"
     CDS             9306..9626
                     /locus_tag="VGB68_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047965.1"
                     /translation="MATTVNKLSRQEQAKSNETTALDQQIARLEEDVRKLKIDFDIFF
                     NGGTKRAPHEARGRVESAIKRIADDRNLAYAQRFHFNTVVARFTSYRELWRRMLKQRN
                     EPGF"
     gene            complement(9772..9978)
                     /locus_tag="VGB68_01680"
     CDS             complement(9772..9978)
                     /locus_tag="VGB68_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEY0047966.1"
                     /translation="MSKALGKVKWFNNAKGYGFIEQPGEQDIFVHYSAISGDGYKTLI
                     QGQEVEFEVINGPKGPQAENVNRV"
BASE COUNT         2376 a         2792 c         2672 g         2438 t
ORIGIN      
        1 ccgcccaagc cttgtccata acgggcgcgg tatcgacgca gattttgcct tcgaccgccg
       61 cgttttcaga ttttatccaa gctaacagct ctcttaattt gtccttgacg acatcgtgat
      121 aatcgtcgcc ccaggcgtag cgcgagactt ttccggtcgg ttcgttttgt tcgtgccggt
      181 gcggcgtgta gtaatttaaa gcgacgacga cgacggattt cgcttccgcg aaaatcagct
      241 tcgggtcggt gcgtttttcc ggctcgcgct ccatccagcg catctcgccg tgaaaatctt
      301 tttcgagcca ttgtttgaga tattcggctt ccggcgcgag cggttcggcg cgggcaacgc
      361 cgattttgtg aaaaccgacc gcaaaggctt tctgtttgat tttttcggtg aaggaagcca
      421 tacgaaaaga ttaacataaa gattttcaga gcaaaaaaag actgactgac cgggaaggtc
      481 aatcagtctt tttataaaaa aattcgaaac gaaagcttta gaaattaagc tttactccga
      541 attggaactg ccgcgggtcg tacgccgccg tcgagcggct gaaatagcgc ccgttcttgg
      601 agcgttcgcc gacagcgttg acgtcgttca gaaacggcga cgcgctggct tcgttgaaac
      661 gattgaaaag gttgaagcct tcggcaatca aatcaagccg gatacgctcg ccgaaacgaa
      721 tcgcgcgcgc aacgcgcaaa tcgaacgagg cgtaaccgtg cgtcaagccc atattgcgtc
      781 cgagattgcc gattgagaat tcgccgttgg taatcagcgg cgtgatgcag ccgagcgttc
      841 cgggctggca caaagtgccg tccgggttaa cgctcggacg gtcggtctgc gttgactggt
      901 cgttgttcgt atcgacgttg gtgatgatat tgaacgggcg accagacgag atttcgataa
      961 tcggcgcgat tgtaaagtcc gccagaaatc tttgccagcc gtttccgccg cgccagcccg
     1021 cgggcgaagc caaaacgccg ctaaagacga agcggtgacg ctggtcaaag agcgaatcgg
     1081 ctttttccaa atcaaagcgc cggacgtcct gcgcgataag caaagtctgc aaatcgctgg
     1141 aatcgtcaat cgaatgcgcc cacgtgtagc ttgcctgaaa cgtcaggtta ttggcaaagc
     1201 gtcgcttcag ctcgacgttc agcgcgttgt attccgaatt accgttggag atttgcgcgt
     1261 tgaccgagcc gaacggcgaa agcgtgcccg gcgttctcaa agaaccggca agcagcgaat
     1321 cgagcaccgg tttggtcaga gcgccgctcg acagcgattg cgccagaaaa taattcggcg
     1381 cgttcggacg gaagaaattg gcaaccgccg gagcaaccac gcgcccgttc ggacactggg
     1441 cgatgatgcc gggaactaaa accgtgcacc cgacgccggt cggcgttccg gctggggtcg
     1501 tcgcaatgga aaaagcaatg gcttgctgcg tagtcgtcgg cagagcgccg ccgaaaaagc
     1561 gttgaaaatt ctgaatttgc agcgccgtat tcggcgtgtt caaatccgtc gggtgcggca
     1621 gattgcgcgc gccgacgaag atatagccga ccgacaaaga catatttgtc gtcaactggt
     1681 gttcaatcgc cagatttgcc tgcacggcgg aagcgtattt gaaatctttc gctatcggca
     1741 gcgtaaacgg cagaaccgga ccgaatccgg taaacgtctg gtcgttgaaa cgctggcgac
     1801 cgaactggta ttgcgccgta gacgccagac cgggcgttat cacgccgggc gcgcaaagcg
     1861 gattcgccgc cgcgccgccg caaaccgttc cctgaaaaac ctgcgccgca ttcagcagcg
     1921 ccgccgggtt cgggctgccc gccgttaaca cagcctgctg ctgctgcgcg gcgtcggcga
     1981 tatccgagtt aaacgcgacg gcaagcagcg gatggtcgta gaaaagaccg atggcgccgc
     2041 gcagcaccgt tctgccattg ccgaaaacgt cgtaagcgac gcccagacgc ggcgcccagt
     2101 tgttcgtatc gcgcggaatt ccctgctgca cgccgagcgc gtcctgcgcc gccagaatat
     2161 cggattccga aagattaatg cccgtcagcg ggtcgcggaa agcgacgggc gcaatcgttt
     2221 ccgtaaactc gacgtcgtag cgaatgccgt aattcagggt taaacgccgg gttgctttcc
     2281 aggtgtcctg cgcgtaaaag gcaatcggtt tgtttttgat tttactaatc ggattgccga
     2341 agccctgaat ataaagcgag ggcaagccga gcccgtagga ttgaaccggc gttaaatccg
     2401 gcaaattcaa cggagcaaaa gacggattga gattcgccgc cgcaaacggt ccgaaattga
     2461 aaagtccggc aaagttcaat tcaaaaagcg ccgacggaat ttccacccag ttaaagtccg
     2521 cgccgaattt gaaagtgtgg tcgccgtaaa cctgagtcag gttgtcggct agctgaaaac
     2581 ggttttccgt gcggtcaacc ggcgagaaaa gctcgcgccc gataaacgcc gtgcccgaaa
     2641 tattgaaagc caccgcttcg ttgttttgcg agcggaagct ggtttttcgt tttccgaaat
     2701 gaaagcgaaa ctcgtttgcc gtatttgcgc cgagcgtcga attaagcccg aaagtaaaag
     2761 ccgtatcttt gattttcgtg atgccggtgc gcgaaaaatc gttttgtccg agcgactggt
     2821 tttgcgattc gacctgaatg ccgctcagat tgctcggatt gtaaccgaaa cgcatcgtta
     2881 actgatggtt ttgattaatg taatggtcgc cgcgcaatga aaaataattg gttctgtccg
     2941 taatcggaaa aaccggctgc aagcccagca gcgggcgaaa cgcaatcggc tcaccgaaat
     3001 tattaaccga attgagcgga atcggcgcgc ccgtcagaaa aaatcgcgag ccaatcaaaa
     3061 aaggcgttcc cggattagga ttgcagagat tggtcggcgt cgccggcggc gtcgggcacg
     3121 gcggcgcgac cagcgggtta actccggtta aagcggtcgt tccgccgccc gaagccagat
     3181 acaggtaacc gactgccgcc tgaatcgcag acggatttcc ggtggctatc aattgctgcg
     3241 ctttttgcgc ctgttccgcg gtcagacgat taaacgtctg accgccgaag gtcaccgaac
     3301 tggtcaggtc tcgcgtcaca tcgctcgtga aaaaccccga ttcgttgcgc tggcggcgct
     3361 caaacgaaaa gaaaaagaac gagcggtctt tcaagagcgg tccgccgagc gtcgcgccgt
     3421 attgcgtgcg cgtaaatgca gttttaaaag gcgcaaacgg attgcgcgcc tggatgcttt
     3481 tatcgcggat aaagccgaaa acgttgccgg taaattcgtt tgttccgcgc ttggtgacga
     3541 cgttgataat gccgcccgtg gcgcgtccga attccggcgc atatgagttg gtcgccacct
     3601 gaaattcctg caccgcttcc tgcgagacgg tcgtgcgcgc ggcgttaatc gagttatccg
     3661 taaaatccga gccgtccacc tgcacgagcg tcgagcgccc gcgctgtccg ccgatgttca
     3721 aacccgatgt aggagcgggt ccaatcgggc gaccgttgtc gcgcccgacg gtcgaaatcg
     3781 tcagcgcaaa tccggtcgcg ctgcgctcat ttataggcag attctcgatt ctctgctggt
     3841 caatcgtgct ggaaacggtt gtgcgcgtcg tttccactaa ttcgacatct ccgcccgtca
     3901 ccgtgacggt ggcgccttcc gcgccgactt cgagccggat ggttaattcc gcgttctgcc
     3961 cgacggttaa tctgacggac gggatgattg tctttttaaa ggttgtggct tccgccgtaa
     4021 tttcgtaagt gctcggcggc aagccgataa atttgtattc gcccgattcg tcgcttgtcg
     4081 tattgcgcgt gattccggtc gccgtatcgc gcgccgtgac ggtcgcgccg ggcaccaccg
     4141 cgccgctcgg gtcggtcacg gtgccgttta aatccgccga gctggcttgc gactgcgcga
     4201 atgccgcgcc ggcgaaaacg agcgtgacca atgccagcgc taaaagacgg ctgacaacag
     4261 tcgtaaatcg caaagaaaac gctctcatta ctttctttct ccttgactaa atttttataa
     4321 ttgccgtcag cccgatgcaa aaccggattg gggatacgtg tcgtctttca ggctgaccag
     4381 taatttctta gaagtgatga ctttaaggtt tgtaacgcaa atttcatcgt aaagcaagca
     4441 aaaaattaca ttttcataac attaaacttc ggctctgaaa aaatatttgt gttttttatg
     4501 aaccgagcgc tgccgcccgc gtcgcgcgcg ccaggattgc ccgcgctttt tcgctccggc
     4561 tcaaaacgga aatcaacgat ttggcaaggc tcggcgcaaa agcggcgcgc cgcatcagcg
     4621 agcagacgaa aagtcttttt cgaaattttt cggcgtgccg ctttttataa atttccgcga
     4681 ttttttccgg cgaaaaattg ttttccgcga cgcttcgcgc cagaagctcg ccgctttcca
     4741 gagccatcag cattccgctg ccggtaaacg ggtcgataaa cgcgccggcg tcgccgattg
     4801 ccagaagccg cggcgcggga tttaaatctt tcgcgccgaa cccgtccacc gaaaccgcga
     4861 gccaatcaaa accggcttcg gcgcttttca tcatctgttc ggcgcgtttg ttttgaaaaa
     4921 caacttcgcg gacgattttt tccgcatcgc cgcccgcctt tttcacgatt tccgattcaa
     4981 tcaaaaaaca atgattcgcg aaaccgtttt cgacgcggct caaaccgccg tagccgccgc
     5041 gaaaaaagta aatttcgcaa acgccgcttt cgattttcgc gtttttcaaa tgcgttttaa
     5101 agccgattaa tttcgggctc cggtctttgg ttttcggtct ttgataattt gaagcttctg
     5161 atttcaaact ccaaattcct ttgcgctttt gcgcctttac gccgccgcgg cgggaaattc
     5221 ccctttcagc cagttttctc aaaatgcctg ctctgcccgt cgcgtcaatc gtcaggcggg
     5281 cggagatttc tttaatctcg ccgtttcggt cgcgggcttt tacgccgccg acgatttcat
     5341 tttcagttaa taagccgagc gcctgagttt cttccaagac ctcgacgccg accgccgcgg
     5401 ctctttcgag cagccgaaaa tccatttccg cgcgacttaa gctcagagcg cccgcgccgc
     5461 cttgaaaaaa ccattcgctc ggcacggcga ccgattttcc gtccggctcg taaaaaacgg
     5521 ttttccgaat ccgctcgccg cccgccgaaa gcatctcgtc gatgacgccc agctcgcgaa
     5581 aatgcgccag acattccggc gagataaatt cgccgcagag cttcgggcgc ggaaattttt
     5641 cgcgctcgac gagcgtcact tgaaaattgt tttgcgccag ccgaatcgcc gcgctcgttc
     5701 cggcaggtcc cgcgccgacg attactattt tttcgcgctc gttttttttc atattcggcg
     5761 gttgccgcca gccggcaaaa atcttgactt tgtctttaat catatactat cgtgataggt
     5821 gagctaacga aatccagccc aatgcgtgaa gtttatatga gagcaggctt cactaaggag
     5881 attgagttac tcgtcggcgc tgttcaaatc aatcaattct ttccgagcaa cgccgcacag
     5941 cttccgaaac agcagcctcc tattcgcttc taattttggg tttcatttca ttgaccgaca
     6001 gcacataaaa ttgtaaaagc cggtaggcaa gccctctttt acaaaaaccg ctgacagttg
     6061 ccccttctta ggctttaatt tcaagaaatt gaaaacggag aaaacaacaa tgaaaaatat
     6121 tctcaagctt agtcgcaagt ttatcgtgtt gggcgcaatg ctttttgctt tgggctttat
     6181 gacttttact aatttcggaa ccgcgcccgc cgacgcttcg ctgttctgct gcagcgaatg
     6241 cgagccgatt tatcaaagct gcatcgacaa cggcggaacg ccgacccaat gcaaccagca
     6301 ggcaaattac tgctggcggc actgcgattt tggttgttaa aatcaatttc gacttgattg
     6361 ttaaaatgtg acgagaggtt gctattatat aatggcagcc tttttatttt atgaaaatca
     6421 ttccgcttgc catcccgacg cctttctata tcggcgacgt caatgtttat ctcatccgcg
     6481 aagagcctct gacgctgatt gacgtcggac cgaaaaccaa agaagccgcc gccgccctgc
     6541 gcgaagggct gcagcgaaac ggcgtcaggt tttccgacat cgaaagaatc gttttgacgc
     6601 acgcgcacga agaccattgc ggtttagcgc gggcagtacg cgacgaagcg aaaaacgcct
     6661 cgattctcgt gcacggttgg gaaaccggac atcttttcgg gcggctggcg cgcgccgaac
     6721 acgaaaatct tttgtatcgc gccggcgttc cgaccgagat ttttcataaa atgcacgatt
     6781 tttacgaaga cgtgcggctt ttgaccgatt cgctttcgga cggcgaattc gatttgctgc
     6841 acgacgagat ggagctggaa ttttcgagcg gcgttttaaa agttctgcac acgccgggac
     6901 acacgcccgg ctcgtgctcg tttatacgcg aatcgaaccg gactttaatc tgcggcgact
     6961 gcgttttaaa acgcattacg ccgaatccgg ttctctcgcc cgacccggtt gacgcccgca
     7021 agcgcttccg ctcgctgggc gaatatctgg tgagcctcgc ccgcctgcgc tcgttttcgc
     7081 cgacgctgac ttacggcggg cacggcgagc cggtcacgga ttttgaggaa atttttaatc
     7141 gctatgtgcg cgctattgac gagcgccaga gaaacgttat tgatttggtg acgccgggcg
     7201 gcgtgacggc gttcgagctt gccttaaagc tttttcccga agccgtcgcg gaaaatgcgc
     7261 agcgttttct cgccgtttcc gaagccgtag cgcacctgga ctacgccgaa tcgcaaggca
     7321 aaatcgcggt cgaattaaag gaaggcgtcg agttttatcg aagattttaa ttgcaaaaaa
     7381 tggattaaaa acctcgaaaa ttcatttttt gcccgctcta aaattgaaat tattccattt
     7441 attcatcttc gcaaatttac cgctgcccgc cggcaatagc taaactttac ttaatacatt
     7501 caaaagctcg gcttcctcag cttcggagac cgttcgtaaa tctatcaaaa ctttgtcttc
     7561 caaaatccgc gcgatgacgg gcggttttga aaggcgcaaa gcttcttcca aacggctgac
     7621 cgtcattttt tcgtgtctga gcgccaaaag cgtcgtcgcg ggctgaacca tcggcgcgga
     7681 gccgccgccg acgaccgatt tgccttccgc gacttcgaat tttaaccggc ggttttcgcc
     7741 taatctttcg cgcagttttc ggtcgaattc gagagttctc gttttgagct cttcgctcgt
     7801 cgtcgaaagc attttcagaa cgggaatttc ctgcaaaacc gtgtcgcggc gaaaggattc
     7861 aaaggtcgct tcgagcgcgg cgtaaatcaa tttatcgacg cgcagggcgc gataaagcgg
     7921 atgagagcgc agtttttcga ccgtctcgcg ccgcccgaca atcaagcccg cctgaacgcc
     7981 gcccaaaagc ttatcgccgc taaacgtcac cacgtcagcg cccgcgacga gcgaccggga
     8041 aataaccggc tcttcgccca aaccgtattc ggacaaatcg agcagcgcgc ccgaaccggc
     8101 gtcttcgtaa agaatcaaat tatttttgtg cgcgacttcg gcaatttcct ttaaatccgg
     8161 cgttgccgta aaaccgacga tgcgataatt cgaagggtga acgcgcagaa gcatccgcgt
     8221 cttttcgttt atcgcgtttt cgtaatcgga gaggcgcgtc cggttggtcg cgccgacttc
     8281 ttttaaaacc gcgccggatt gcgccagcac ttcgggaacg cgaaagtcgc cgccgatctc
     8341 gaccagctcg ccgcgcgaga ttatcacctc gccgccgctc gccagcgccg tcagaaccag
     8401 aaacgccgcc gccgcgcaat tgttgacgat cagagcgctc tccgcgcccg ccagcgccgc
     8461 cagcagatgt tcggcgcgcg cgccgcgccg cccgcgcttt ccggtttcga gattgtattc
     8521 cagattgcag tagcgcgcgg ctttctcaaa gattgctttt cgggcatttt ccgaaagcgg
     8581 cgcgcgtccc agatttgtat gaataatcac gccggtcgcg ttgacgactc tctgcaaacc
     8641 gacgttttgc tcgctttgcc agagctcggt caggcgtttt tcggcttcgt ttaataaatc
     8701 ttcgcgcgag tatcggagca tttcgccgcc ggcgattttt tcctgcaaat tccgcctgag
     8761 caaatccgtc accgcccgcg ccagcgtcgc cagatgacgc gcgccggttt gcgaaatcaa
     8821 tttctgcgcc gcctgcgtcc gcaaaagcgc gtcaatcgaa ggcagaagcc gcagtgcttc
     8881 ggtgtggttt ttcgtttccc gattttccat aagcgcattt taacttaata accggaatta
     8941 gcgacagaga gcacgcgaag aacaaagata tttaagatga cagagagctg atagattccg
     9001 tgtcaaaggc aattcgcaaa cgtttacttt tattgaagcg aggctttcga cgccggcacg
     9061 ctccgaacgc ggcgcattaa aaccttcgcg gtcgccgttg caaaccacgc ggtcgccatg
     9121 gtagttgtcg cgcccgtcgg cgtgtcgggc tggcgtttga gccggttttg cgccaaaaag
     9181 acgctttttg attcttttag attcttttag cggaataacg ggcagtttga agattttata
     9241 tatctggaga gaattttctc caatcatctc gaagtttctc caatcatccc aggaaggttt
     9301 tgaaaatggc aactactgta aacaaattat cacggcagga acaagctaaa agcaacgaaa
     9361 cgaccgcgct cgatcagcaa atagcccgac tcgaagaaga tgtgcgaaag ctcaaaatag
     9421 atttcgacat ctttttcaac ggcggcacga aacgcgcgcc gcacgaagct cgcggtcggg
     9481 tcgaatccgc catcaaacgc atcgccgacg accggaatct ggcttacgcg cagcgctttc
     9541 atttcaacac ggtcgtcgcg cgatttacgt cctaccgcga gctctggcgt cgaatgctga
     9601 agcagcgcaa cgagccggga ttctgaaagg cgaaaggcta aaggatgaag gatgaagctt
     9661 ccgagcaaat agttttgcct cttttcttca gcctttcgcc tttagccttt cgccttttga
     9721 aatacttcgg ctcaaaagaa accgaaagcc atcaaatgag tttaattttt gctagacacg
     9781 attgacgttt tccgcctgcg gacctttcgg tccgttgatg acttcaaact cgacttcctg
     9841 tccctgaatc agggttttat aaccgtcgcc ggaaatcgcg ctgtaatgca cgaaaatatc
     9901 ctgttcgccg ggttgttcga taaaaccgta acccttcgca ttgttaaacc atttgacctt
     9961 accgagtgct tttgacattt ttttgtaact cctcctacaa acttcagtga atttaaaata
    10021 gtaaacactt gcaaaaagcg agtgttttca gaaaaaaagt tttgagccgg aagcatttcc
    10081 taaacgaaac acgggcaaaa aacaaaatct acgccgagca ggacgcgctt cgtccaactc
    10141 atcgacgcag attaaacgaa aatctaatac aactcatttc ctaacgaaaa gaaaatctta
    10201 aactgcaaga ttattgggat tttgatgtgt tgtcttttga tagaacgacg gaaaatttat
    10261 ctgatttaca agcctctg
//
LOCUS       DASWNE010000081         1990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_639348, whole genome shotgun sequence.
ACCESSION   DASWNE010000081 DASWNE010000000
VERSION     DASWNE010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639348"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VGB68_01685"
     CDS             <1..200
                     /locus_tag="VGB68_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047967.1"
                     /translation="KSSAWLLGSIGTDKYRARKRFLRAVGKLGLTYWHIQRATDAQGQ
                     TASFQQNPILREEVLKWRDKV"
     gene            676..1821
                     /locus_tag="VGB68_01690"
     CDS             676..1821
                     /locus_tag="VGB68_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0047968.1"
                     /translation="MNLELSEEQQMVRASVREFAQNEIAARAREYDEAEKFPKEQIDG
                     LAELGLLGMIIPEEYGGAGFDTVSYALALEEIAKADASVAVIVSVTNSVCCYPILSYG
                     TEQQKQKYLLPLAKGETLGAFCLSEPQAGSDAANQKTRAVETGDYYILNGTKSWVTNG
                     GIAGVYLVMAVTGEKEGRKEITAFLIDGDAEGLKVSSVEHKLGQRASQTTEMSLSEVK
                     VPKANVLGGVGNGLKVAFGSLDNGRIGIAALSVGIAQAALEESAGYAKDRTAFGQPIA
                     EFQAIRHKLADMHTETEAARLLTLRAAALKDAGARNVGVFSSMAKLFASENANRVCAE
                     AIQIHGGNGYSRHYNVEKYYRDARITTIYEGTSEIQRIVISRGILKE"
BASE COUNT          552 a          474 c          507 g          457 t
ORIGIN      
        1 ttaaaagctc ggcgtggctt ctgggcagta tcggtacgga taaatatcgg gcgcggaaaa
       61 gatttctgcg cgccgtcggc aaactcggat tgacctactg gcatattcaa cgcgccaccg
      121 acgcgcaggg acaaaccgcc agctttcagc aaaatccgat tctccgcgaa gaagtcttaa
      181 aatggcggga taaagtttaa aagcgaaggc gggaaaaagt ttaaaagata aagtttaaaa
      241 gtgaaaatcc aaaagcgaaa aagtgaaaga gtgaaaaagt ttgttgactt ttatcaaccc
      301 gctttttcac tctttcactt tttcactctt aaactttaaa cttttaacat ttaccgagca
      361 gccagaaaac gaccggaaat ataataacca tcgaaaaaat gccgccgccg aatttccacg
      421 ccgccgcgcc tgctatcaat ccttttaacg gtccgttcat atttttaacc ttcccctgtt
      481 cttaaattta aaatcgtctg cataaaaata atacagaaaa ttattgataa tcaggctccg
      541 gcaaaagacg tttcatgaaa gcgaaagacg gataatttaa aacttaatat ttcgggtcgt
      601 ccgtttttcg ctttccttta ttcatttttc gttatccatt tcttattttg cctgtctcgc
      661 tttaaacttg attctatgaa tctggaattg agcgaagagc agcagatggt gcgcgccagc
      721 gtgcgcgaat ttgcgcagaa cgagattgcc gcgcgcgcgc gcgaatatga cgaggcggag
      781 aagtttccaa aagaacagat agacggttta gccgagctcg gacttttggg aatgattatt
      841 cccgaagaat acggcggcgc aggattcgac acggtttcct acgcgctggc gctggaagaa
      901 atcgccaaag ccgacgcttc ggtcgccgtc attgtttcgg tgacgaactc ggtttgctgt
      961 tacccgattt tgagctacgg gacggagcag cagaaacaga aatatctgct gccgctggcg
     1021 aaaggcgaaa cgctcggcgc gttttgtctg agcgagccgc aagccggctc ggacgcggcg
     1081 aatcaaaaaa cgcgcgctgt cgaaacgggc gattattaca ttctgaacgg cacgaaatcg
     1141 tgggtcacga acggcggaat cgcgggcgtt tatctggtga tggcggtcac gggcgaaaag
     1201 gaaggcagaa aggaaatcac cgcgttttta atcgacggcg acgcggaagg cttaaaagtt
     1261 tcgtccgtcg aacataaact cggtcagcgc gcttcgcaaa cgaccgagat gagtttgagc
     1321 gaagtgaaag ttccgaaagc aaacgtcctc ggcggcgtcg gcaacgggct gaaagtcgct
     1381 ttcggctcgc tcgacaacgg tcgaatcggc atcgccgctt tatcggtcgg catcgcgcaa
     1441 gccgctctgg aagaatccgc cggatacgcc aaagaccgca ccgctttcgg tcagccgatc
     1501 gccgaatttc aggcaatccg gcacaagctc gccgatatgc acaccgaaac cgaagccgcg
     1561 cgcttgctga ccctgcgcgc cgccgctttg aaagatgccg gcgcgcgaaa cgtcggcgtt
     1621 ttcagctcga tggcaaagct tttcgcttcg gaaaacgcca accgggtctg cgccgaagcc
     1681 atccagattc acggcggcaa cggttattcg cgccactaca acgtcgaaaa atattaccgc
     1741 gacgcgcgca tcacgaccat ttacgaaggc acgagcgaga ttcagcgcat cgtgatttca
     1801 cgcgggattt tgaaggaata gagtataata aaacttgata tttaaatgag gtcgatcagt
     1861 agaaatgaat aatattaata gccgcgaaga tgaaaatttt ttagatattg atacggaaac
     1921 taaagattgg acattagaga taattttagg cgctgttgag gcaggagttg aagtccctat
     1981 taccttgaat
//
LOCUS       DASWNE010000082         4266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_462379, whole genome shotgun sequence.
ACCESSION   DASWNE010000082 DASWNE010000000
VERSION     DASWNE010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462379"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VGB68_01695"
     CDS             <1..305
                     /locus_tag="VGB68_01695"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY0047969.1"
                     /translation="GNPLAFHWTWNAAICVLYLAIMGSIVAFWLYYWLLTKIDVTRAM
                     MIAFVTPLVAVIIGGFFGEKLGLQTLFGGLLILLSVFLIVVRPILNHRNSVANQRL"
     gene            310..1482
                     /gene="aroC"
                     /locus_tag="VGB68_01700"
     CDS             310..1482
                     /gene="aroC"
                     /locus_tag="VGB68_01700"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEY0047970.1"
                     /translation="MFFRFTTAGESHGKALVAIVEGLPAGLEIDIEKINQELWRRQQG
                     YGRGGRQKIESDKIEILSGVRHGKTLGSPVALVIENRDFVHWTEVMSAEKPDSEPKNP
                     RLVKRPRPGHADLAGGQKFQTRDLRDILERASARETAARVACGAIAKQLLGQFEIEIR
                     SHVIKLGSVPEKPLQKNWEEICAVEKDSPLNCADSEVEKAMVELIDKAKADGDTLGGV
                     FEVVAKRVVAGLGSHTSWSEKLDGRIAQAFMSIQAVKAVEIGEGVPNAGRFGSMVHDE
                     IGYDESSKTFTRPTNRAGGLEGGITNGEELRVRGYLKPISTLRKALKSVDIESKQEEL
                     AAFERSDITAVPAAGVIGESMLAIVLANAMREKFGGDSMEEMRRNFENYKKALQNY"
     gene            1604..2137
                     /locus_tag="VGB68_01705"
     CDS             1604..2137
                     /locus_tag="VGB68_01705"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEY0047971.1"
                     /translation="MNKSLKAKLCFFILSIAVLTGATGADISAQSRVYTASVNARVSA
                     NADADEREIFELINQERGKKGLGELNWDSNLSQLARAYSQKMARENFFSHYDGDGGSV
                     ETRARDMRIKNWSKIGENLFFCEGYGNPNVMAVRGWMKSPSHRENILNPDYTDSGIGI
                     AVAGGGRIYVTQVFIER"
     gene            2310..3314
                     /locus_tag="VGB68_01710"
     CDS             2310..3314
                     /locus_tag="VGB68_01710"
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="HEY0047972.1"
                     /translation="MDFLFDNPLANMPGIIFLIFYSLLAVFSLGIFGIWKMQADKTAS
                     LPVPPIPPNIDPYEIAYLRGGMNEVARAAVFALRQKDFLIIQTDGKNSRIHRNQHQTD
                     KRSLNQLEQTVLNWFGVSREPKEVFQGYGLIQVLEPYYGVYESRLEQQQFLTGDETRS
                     RVKRARNIVLLLILGFGAYKFVAALINGYLNVFGIIIVAVIATLITFAVARLPRLTNL
                     GKTYLERLQLAFDRLKQQTQQAYQLSGTPKVLPSAQFAGADPLLLSVGVFGGAVLAGT
                     IYDDYNQAFERAQNQAAASGSSGCGSACGSGCSSSSGGDGGGSSCGGGCGGCGGGGGD
                     "
     gene            3326..4153
                     /locus_tag="VGB68_01715"
     CDS             3326..4153
                     /locus_tag="VGB68_01715"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HEY0047973.1"
                     /translation="MGGSFQNLPKHGVGIGFREQFRADLFLHQAEIDFLEITADHYLD
                     ATAQKLEELELLRQHFPLIPHGLNLSLGSAEGVDEKYLEKLAALIEKLQPAWFSEHIC
                     FTKSGGIDIGHLAPLAFTRESLDVLKRNVGRVRQFIKTPLILENITYMIRFPFSEMDE
                     AEFITELIEETDCGLLLDVTNLYINSINHGYDWRGFLEKLPSERIVQLHFVGSHRHGN
                     LLIDGHADKTEDEIWNVFREVCVKSNVKGAILERDEKFPPFAEILQELETARNLLKN"
BASE COUNT         1070 a         1042 c         1140 g         1014 t
ORIGIN      
        1 aaggtaatcc gctggcgttt cattggactt ggaatgcggc gatttgcgtt ttgtatctgg
       61 cgattatggg ttcaatcgtc gctttctggc tttattactg gctcctgact aaaatcgatg
      121 tcacgcgggc aatgatgatt gcctttgtaa cgccgctggt agcggtgatt atcggcggat
      181 ttttcggcga gaaattaggg ctgcaaactt tgttcggcgg tcttctgatt ttgttgagcg
      241 tttttctgat tgtcgtccgc ccgattttga atcacagaaa ctcggtcgct aatcaacgtt
      301 tataaattta tgttttttcg tttcacgaca gccggagaat cacacggcaa agcactggtc
      361 gcgattgtcg aaggtttgcc cgcgggtttg gaaatcgaca tcgaaaaaat caatcaggag
      421 ctatggcgtc gccagcaggg ctacgggcgc ggcggcaggc agaaaatcga aagcgacaaa
      481 atcgagattc tttccggcgt tcgtcacgga aaaacgctcg gctcgcccgt cgcgcttgtc
      541 atcgaaaatc gcgatttcgt tcattggacg gaagttatgt cagcggaaaa gccggacagc
      601 gagccgaaaa atccgcggct cgtcaaacgc cctcgtcccg gtcacgccga cctcgcaggc
      661 gggcaaaaat ttcaaaccag agatttgcgc gatattctgg agcgcgcttc ggcgcgcgag
      721 accgccgcgc gcgtcgcctg cggcgcaatc gccaagcaac tgctcgggca attcgaaatc
      781 gaaatccgct cgcacgtcat caaactgggc agcgttcccg aaaagcctct gcaaaaaaat
      841 tgggaagaaa tctgcgccgt tgaaaaagat tcgcctttga actgcgccga ttccgaagtt
      901 gaaaaagcga tggtcgagtt gattgataaa gcgaaggctg acggcgacac tctgggcggc
      961 gtttttgagg tcgtggcgaa acgggtcgtc gccggtctgg gctcgcacac ttcgtggtcg
     1021 gaaaagctcg acgggcgaat cgcgcaggcg tttatgtcga ttcaagccgt gaaagcggtc
     1081 gaaatcggcg aaggcgtccc gaacgcgggt cgtttcggtt caatggtgca cgacgaaatc
     1141 ggatacgacg aaagctcaaa aacctttacg cgcccgacca atcgcgcggg cgggctcgaa
     1201 ggcggcatca cgaacggcga agagctgcgc gttcgcggtt atctgaaacc gatttcgact
     1261 ttgcgaaagg ctctgaaatc ggtcgatata gaatccaaac aggaagagct cgcggcgttt
     1321 gagcgctcgg acattacggc ggttccggcg gcgggcgtta tcggcgagtc aatgctggcg
     1381 attgttttgg caaacgcgat gcgcgaaaaa ttcggcggcg attcgatgga agaaatgcgc
     1441 cgcaatttcg aaaattataa aaaagcttta caaaattatt aaattcgata aaaatagaaa
     1501 gccggagctt tagtctttgc atcaaagcgc gaaagctcca atccggttct cccgaacccg
     1561 aaactctctc cgaaccaatt ttcaagaagg aaaaatttaa tcgatgaata agtctttaaa
     1621 ggcaaaattg tgctttttta ttttgtccat cgccgtttta acgggcgcga cgggcgcgga
     1681 catttccgcc cagagcagag tttacacggc ttcggtcaac gcgcgggtct ccgccaatgc
     1741 ggacgccgac gagcgggaaa tcttcgagct tatcaaccag gaaagaggca aaaaaggctt
     1801 gggagaatta aactgggact caaatctttc gcagctggcg cgcgcctatt cgcaaaaaat
     1861 ggcgcgggaa aattttttca gccattacga cggcgacggc gggtcggtcg aaacgcgagc
     1921 cagagatatg cgcatcaaaa actggagcaa aatcggcgaa aatcttttct tctgtgaagg
     1981 ttacggtaat ccgaacgtaa tggcggtgcg cggctggatg aaatcgccct cgcatcgtga
     2041 gaacatcctc aaccccgatt acaccgattc gggcatcgga attgccgtcg ccggcggcgg
     2101 cagaatttac gtcacgcagg tttttatcga acgatagtta taaaattcaa aaaatcgcaa
     2161 tgcaaagctc aaagggaaat tacgtctttc atcctgagct ttgcatttcg atttttgcgt
     2221 tgttaacttt ttgttacatt tcgaacccga atctctggta attcgtaata gaaagtggct
     2281 agaataagcg cgaggaggaa atttaccgaa tggacttcct gtttgataac ccgctggcaa
     2341 atatgccggg catcatcttt ttaatctttt acagcctgtt ggcggttttc tcgctgggca
     2401 tattcggcat ctggaaaatg caggctgaca aaaccgcgag cctgcccgtg ccgccgattc
     2461 cgccgaatat cgacccgtat gaaatcgctt acctgcgcgg cggaatgaac gaagttgccc
     2521 gcgccgccgt ctttgcgctg cgccaaaaag attttctgat aatccagacg gacggcaaaa
     2581 attcccggat tcatcgcaac cagcatcaaa ccgataagcg aagcctgaac cagttggagc
     2641 aaaccgttct gaattggttc ggcgtttcgc gcgagccgaa ggaagttttt cagggctacg
     2701 ggttgataca ggttttagaa ccgtactacg gcgtttacga atcgaggctg gaacagcagc
     2761 agtttttaac cggcgacgaa acgcgaagcc gcgtcaagcg cgccagaaat atcgttctgc
     2821 tcctgatttt aggtttcggc gcatacaaat tcgtcgccgc cttaatcaac ggctacttga
     2881 acgttttcgg gattattatt gtcgccgtca tagcgacttt aataaccttt gccgtcgcca
     2941 gattgccgcg tctgacaaat cttggaaaaa cttatctcga aagactccag ttggctttcg
     3001 acagattaaa acagcagacg cagcaggctt atcaactgtc cggcacgccg aaagttttgc
     3061 cgagcgcgca gtttgccgga gccgacccgc tgcttttaag cgtcggcgtt ttcggcggcg
     3121 cggttctggc gggaacaatc tacgacgatt acaatcaggc gtttgaacgg gcgcagaatc
     3181 aagcggcggc ttccggttcg agcggctgcg gctcggcttg cggttccggt tgttcgtcct
     3241 caagcggcgg tgacggcggc ggcagttcgt gcggcggcgg ttgcggcggt tgcggcggag
     3301 gcggcggcga ctaaattcgg attctatggg cggctctttt caaaatcttc ccaaacacgg
     3361 cgtcggcatc ggctttcgcg agcaattccg cgccgatttg tttttgcatc aggcggaaat
     3421 tgattttctc gaaatcacgg ctgaccatta tctcgacgcg accgcgcaaa agctcgaaga
     3481 attagaactg ctcagacagc attttccgct gattccgcac ggcttgaatc tctcgctcgg
     3541 cagcgccgaa ggcgtggacg aaaaatatct ggaaaagctc gccgcgttaa tcgaaaagct
     3601 acagcccgcc tggtttagcg agcatatctg tttcaccaaa tccggcggca tcgacatcgg
     3661 gcatctcgcg ccgctcgcct tcacgcgcga aagcctggac gttttaaaac gcaacgtcgg
     3721 gcgcgtcagg caatttatca aaacgcctct gattctcgaa aatataactt atatgattcg
     3781 ctttccgttt tcggaaatgg acgaagccga atttatcacg gaattaatcg aggaaaccga
     3841 ttgcggttta ttgctcgacg tgacgaatct ttatatcaat tcaatcaatc acggttacga
     3901 ctggcgcgga tttctggaaa aactgccctc ggagagaatc gtccagcttc attttgtcgg
     3961 ctcgcaccgg cacggaaatt tgttgattga cggtcacgcg gacaaaaccg aggacgaaat
     4021 ctggaacgtt tttcgcgaag tttgcgtaaa atctaatgtc aaaggcgcga ttctggaacg
     4081 cgacgaaaaa tttccgcctt ttgcagagat tttgcaggaa ctggaaacgg cgcgaaatct
     4141 gttgaagaat tagaatttcg ttagaaattt taacgtataa attttaataa aatgacgaat
     4201 ccgaaatccg acattttttg tccgaattgc agcgcaaaaa ataagattga acaaaacttt
     4261 tgccgt
//
LOCUS       DASWNE010000083         2330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_109232, whole genome shotgun sequence.
ACCESSION   DASWNE010000083 DASWNE010000000
VERSION     DASWNE010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109232"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..301
                     /locus_tag="VGB68_01720"
     CDS             92..301
                     /locus_tag="VGB68_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047974.1"
                     /translation="MKKIAFTLFLAFVFVFSLSINAFAEGNIPNGGRNCGYEGVPPCL
                     VAPIEPTEDTTYYKTVIDYLAQLFG"
     gene            611..1453
                     /locus_tag="VGB68_01725"
     CDS             611..1453
                     /locus_tag="VGB68_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047975.1"
                     /translation="MSIKKFYKVLLLVSLFCAAGCTKAQTTGEITGAPTPKNAEIPKI
                     QNPGKVIHVLVALCDNENQGIVPVPKFLGNGEDPARNLYWGAAFGVKAFFSKSASWTK
                     ITEIKNPSANVLERIVFKHKTQNVYLVADAYRGSRMRETIDDFFAATAGAKRENVEAQ
                     SQTLQVFATADLIAFVGHNGLMDFALENQPAKRDEAARDAVILACASRSYFGEPLRRT
                     SAQPLLWTTNLMAPEAYILHDALEGWTRGESREQIRARAAQAYSKYQKISLKAAQNLL
                     VTGW"
     gene            1536..>2330
                     /locus_tag="VGB68_01730"
     CDS             1536..>2330
                     /locus_tag="VGB68_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047976.1"
                     /translation="MTFILSLAAPAQETEKWTKTASPKGDFIAQLPPDFLVDNEDDAY
                     RVYAFHNEATMRILIDEASDAKSRLKMLRQVSGAREARVTQFDIGGFSGKMYTYEREK
                     SISVSIYLASSKAFITVSAWAKEESNAELKNFLYSIRANNQALFKNPPLINQDAEEPI
                     SVAALRTSPIVLSMLKIKDAEKTPVKYALEDPKKKKDDEEEGDDDNADKYSRPLITLR
                     KPRASYTDTARGNNVQGIVRLKIVFRSDGRIGEIIVLQKLDGSLTAE"
BASE COUNT          649 a          533 c          575 g          573 t
ORIGIN      
        1 tatctttgca agatttcttg ctaatatgat aaagcggtat tttatcaaca cttaccgacc
       61 aaaaaacatc taatttctag gagaaaataa aatgaaaaaa atcgctttca cgctgttctt
      121 agcgtttgtc ttcgtgtttt cactttcaat caacgcattc gccgagggca atattccgaa
      181 cggcggcaga aattgtggat atgaaggcgt tccgccctgt ctggtcgcac cgattgagcc
      241 aaccgaagac acgacttatt acaaaactgt gattgattat ttagcacagt tgttcggtta
      301 atagcaaccg attttttaag ttttttttag cctcttattg ccgtttattc gggaggtttt
      361 tgattagtgt gtccgttttt ctctttttcg atgttggaag ttagccgtcc gtcctcaatc
      421 ttgataaatt cggctgtggg gaaatttgtg gggagtctgt gcttgtatgg agttgtaaac
      481 tgttttgcga agtgtaagaa aaacaaggct ttgcggtttg gtgcaatttt atgcaaagca
      541 ttggaaaaga atatgctttt ctttcattgc tataatgcgc ccaaatgaag tttgaaagat
      601 gaaaacgccc gtgtcaatta aaaaattcta caaagtcctg ctcttagttt cgcttttctg
      661 cgccgccggt tgcacgaaag cgcagacgac cggcgaaatt accggcgcgc cgacgccgaa
      721 aaacgcggaa attccgaaga ttcaaaatcc gggaaaagta attcacgtgc tggtcgcgct
      781 gtgcgacaac gaaaatcagg gcattgtgcc cgttccgaaa tttctgggaa acggcgaaga
      841 cccggcgcga aatctttact ggggcgcggc tttcggcgtg aaagcttttt tctcgaaatc
      901 cgcaagctgg acgaaaatca ccgaaataaa aaatccgtcg gcgaatgttc tggaaagaat
      961 cgtttttaaa cataaaacgc aaaacgttta tctcgtcgcg gacgcttatc gcggaagccg
     1021 aatgcgcgaa acgattgatg atttttttgc ggcgacggcg ggcgcaaaac gcgaaaatgt
     1081 cgaagcgcaa agccaaacgc tgcaagtttt tgcgacggct gacctgattg cctttgtcgg
     1141 gcataacggc ttgatggatt tcgcgctcga aaatcagccc gcgaaacgcg acgaggcggc
     1201 gcgcgacgcc gtgattctgg cttgcgccag ccgcagttat ttcggcgagc cgctgcgcag
     1261 gacttcggcg cagcctttac tctggacgac gaacttaatg gctcccgaag catacattct
     1321 gcacgacgcg ctcgaaggct ggacgcgcgg cgagagccgc gagcagatcc gcgcccgcgc
     1381 cgcgcaagcc tacagcaaat atcaaaaaat cagcttaaaa gcggctcaaa atcttctggt
     1441 gaccggctgg taagcagata taaaaataaa atccccggcg gcaaaatcat ctctgaaagt
     1501 aaataacaca ttatgaaaat aataatactc tttttttgac ctttatttta tctctcgccg
     1561 cgccggctca ggaaacggaa aaatggacga aaacagccag tccgaaaggc gattttatcg
     1621 cgcagcttcc gccggatttt ctggttgaca acgaagacga cgcctatcgc gtatatgctt
     1681 ttcataacga agcgacgatg agaatcctga ttgatgaagc ctctgacgca aaatcgcggc
     1741 tgaagatgct gcggcaagtt tcgggcgcgc gggaagcccg cgttacgcag tttgatatag
     1801 gcggtttcag cggcaaaatg tatacctacg aaagagaaaa aagcatttcg gtgtcaatct
     1861 acctggcttc gtcaaaagct tttattaccg tctcggcttg ggctaaagag gaaagcaacg
     1921 cggagctgaa aaattttttg tattcaatca gggcgaataa tcaggcgctt tttaaaaatc
     1981 cgcctttaat caatcaggac gccgaagagc cgatttcggt cgccgctctg agaaccagcc
     2041 cgatcgttct cagtatgtta aaaattaaag acgccgaaaa aacgccggtc aaatacgctc
     2101 ttgaagaccc gaaaaaaaag aaagacgatg aagaggaagg agacgacgac aacgccgaca
     2161 agtattcgcg cccgttgatt acgcttcgca agccgcgcgc gtcatacacc gacaccgcca
     2221 gaggaaacaa tgttcaggga attgttcggc tgaaaatcgt tttccgttcg gacggtcgga
     2281 taggcgagat tatcgttttg caaaaactcg acggcagttt gaccgcagaa
//
LOCUS       DASWNE010000084         3864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_38390, whole genome shotgun sequence.
ACCESSION   DASWNE010000084 DASWNE010000000
VERSION     DASWNE010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38390"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="VGB68_01735"
     CDS             complement(<1..682)
                     /locus_tag="VGB68_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047977.1"
                     /translation="MRKIKAFILLSLFLLTTSSVAVFAQSKKPGDVLIRNATVLTAAR
                     GTLPETDILIQNGRIARIGKNLSAPSNAQVIDGSGKFVSPGIIDCHSHSMLDAINEFS
                     FSVTSMTRMQDVLNPTDITIYRALAGGVTTANLLHGSANAIGGQNTVVKFKYGRPVED
                     FIFTGAPAGIKFALGENPKRTNFNPGAGQQARYPRTRMGTMEVIRDAFLRARDYKQSW
                     DDFRAKKTK"
     gene            complement(710..2368)
                     /locus_tag="VGB68_01740"
     CDS             complement(710..2368)
                     /locus_tag="VGB68_01740"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0047978.1"
                     /translation="MKKLILRFTLAIFAFISLGALPLAAQVSGSFAITNARIVTVSGA
                     AIERGTIVIRDGLIESVGENARVPADARVFDGVGLTVYPGFFDANTNLGLPPPPARPP
                     GQTSPQQQAQTPQPSNSNYPNGLQPETSALDALKAGDAQFETQRNNGITTVLTVSRDG
                     IFNGQSAVINLAGEAVSTMVIKSPFAQHITFRTLPGGTYPTALMGTFSAIRQMLLDAQ
                     RLQEWQKNYALNPRGMKRPDFDKSLEALLPVLNRQMPVVFNANTEREIVRALNLAKEF
                     NLRAIISGGNEAWKVAGRLKEQNVPVLLSLNFPKRTTVASPDADPEDLEVLRLRAETP
                     GGAARLAQAGVKFAFQSGGMPNITDFWTNAGKTIENGLNKDAAIRAMTLATAEIFGVE
                     NRLGSIEQGKIANLTISRGDIFSKDKAITHVFVDGKLFEQKVPERKPTATTGAITTTT
                     AKIDVSGAWNMTVELPGQSVPVTLSLKQEGEKLSGSVQSGFFGTAQIRNGSVTGDSFS
                     FDVTIAVGGQNMDVAFTGKVSGNQISGAATSQQGATPFSGTKVP"
     gene            2505..2906
                     /locus_tag="VGB68_01745"
     CDS             2505..2906
                     /locus_tag="VGB68_01745"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HEY0047979.1"
                     /translation="MSAVILFDGVCNFCNGAVNFIIDRDKAGYFKFAPLQSEIGEKLL
                     AENGIDKIETDSVVLIEDGKVYTHSTAALRVARKLDGAWKFTYGFVVVPRFVRNFFYR
                     LFAKYRYKMFGKQDACMLPTPEIRQRFLAMS"
     gene            3423..3704
                     /locus_tag="VGB68_01750"
     CDS             3423..3704
                     /locus_tag="VGB68_01750"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEY0047980.1"
                     /translation="MKAIWNGAVLAESDETIVVEGNHYFPESSINKEYFQPSQTHTVC
                     GWKGTASYYDVVANGATNKDAAWFYPDAKTEAKEIENYVAFWKGVEVKA"
BASE COUNT          841 a         1009 c         1035 g          979 t
ORIGIN      
        1 tttttgtttt cttcgcgcga aaatcgtccc acgactgttt gtaatcgcgc gcgcgcagaa
       61 aggcgtcgcg gataacttcc atcgtgccca ttctggttcg cgggtaacgc gcctgctgtc
      121 ctgcgccggg attaaaattc gtccgtttcg ggttttcgcc gagcgcgaat ttgatgccgg
      181 cgggcgcgcc cgtaaaaata aaatcctcga ccgggcgacc gtatttgaat ttgacgaccg
      241 tattctgtcc gccgatggca tttgccgagc cgtgcagaag attcgccgtc gtcacgccgc
      301 ccgccagcgc gcggtaaatc gtgatgtcgg tcggattcag aacgtcctgc atccgcgtca
      361 tcgaggtcac gctgaacgaa aactcgttta tcgcgtcgag catcgagtgc gagtgacagt
      421 cgataattcc gggcgagacg aattttccgc ttccgtcgat gacctgcgcg tttgaaggcg
      481 cgctcaaatt ttttccgatg cgcgctatgc gcccgttttg aatcaaaatg tccgtctccg
      541 gaagcgttcc gcgcgcggcg gtcaaaacgg tcgcgttgcg aatcaaaacg tcgcccggct
      601 ttttcgactg cgcgaaaacg gcgacgctcg acgtcgtcag caaaaataaa ctcagaagaa
      661 taaaagcctt tatttttctc attctcggtt cttccctttt tctaaactct tatggaactt
      721 tcgttccgct aaacggcgtc gcgccctgct gtgaagttgc cgcgccgctg atttgattgc
      781 cgctgacctt tccggtaaaa gccacgtcca tattctgacc gccgacggcg atcgtgacgt
      841 cgaagctgaa gctgtcgccg gtcaccgagc cgttgcgaat ttgcgccgtt ccgaaaaatc
      901 cggactgcac gcttcccgaa agcttttcgc cttcctgttt cagactaagc gtcaccggca
      961 cgctctgacc gggaagctcg accgtcatgt tccacgcgcc gctgacatct attttcgccg
     1021 tagtcgtcgt gattgcgccg gtcgtcgcgg tcggcttgcg ctcgggcact ttctgctcga
     1081 acagctttcc gtccacgaaa acgtgcgtaa tcgccttgtc ttttgaaaaa atatcgccgc
     1141 gcgaaatggt caggttggcg atttttccct gctcgattga cccgagccga ttctcgacgc
     1201 cgaagatttc cgccgtcgca agcgtcatcg cccggatagc cgcgtcttta tttaaaccgt
     1261 tttcaatcgt ctttccggcg tttgtccaga aatccgtgat gttcggcatt ccgccggatt
     1321 gaaacgcgaa tttaacgccc gcctgcgcca gccgcgccgc gcctccgggc gtttccgcgc
     1381 gaagccgcag aacttccaaa tcttccgggt cggcgtcggg cgaagcgacg gtcgtgcgtt
     1441 tcggaaaatt cagcgaaagc agaaccggaa cgttttgttc ctttaatctg cccgccactt
     1501 tccaggcttc gttgccgccg gaaataatcg ctcttaaatt aaactccttc gccaggttca
     1561 gagcgcgaac gatttcgcgc tccgtgttcg cgttaaaaac gaccggcatc tggcgattca
     1621 aaaccggaag cagcgcttcg agcgatttgt cgaagtcggg acgcttcatc ccgcgcggat
     1681 tcaaggcgta atttttttgc cattcctgaa gccgctgcgc gtcgagcagc atctggcgaa
     1741 tcgccgaaaa agttcccatc agagcggtcg gatacgtgcc gcccggaagc gtccggaaag
     1801 tgatgtgctg ggcgaaaggc gatttgatga ccatcgtcga gaccgcttcg ccagccagat
     1861 tgatgaccgc cgattgaccg ttgaaaatgc cgtcgcgcga gacggtcaaa acggtcgtga
     1921 tgccgttgtt gcgctgcgtt tcaaactgcg cgtcgcccgc tttaagggcg tccagcgccg
     1981 aggtttcggg ctgtaagccg ttcgggtaat ttgaattcga cggctgcggc gtctgcgcct
     2041 gttgctgcgg cgacgtttgc ccgggcggtc gcgcgggcgg cggcggcaag ccgagattcg
     2101 tattggcgtc gaaaaagccc ggataaaccg tcagaccgac gccgtcgaaa acgcgcgcgt
     2161 cagccggaac gcgcgcgttt tcgccgaccg attcgatcaa gccgtcgcgg atgacgatcg
     2221 tgccgcgctc aatcgccgcg cccgagaccg tcacgatgcg cgcgttggta atcgcaaacg
     2281 agccggagac ttgcgccgcg agcggaagcg cgccaagcga gataaacgca aaaatagcga
     2341 gcgtaaaacg caggataagt tttttcataa ctttatttgg aagctgaaaa cggatttgca
     2401 aactcgtgtt acgcattttc tcgtaaaagg gtttcagttg caaatgaaat tttgttttcc
     2461 gcgcggctat ttacgattca cgtcgaaagt tttaccattg aaatatgagc gcagtaattt
     2521 tattcgacgg agtttgtaat ttctgcaacg gcgcggtgaa ttttattatc gaccgcgaca
     2581 aggctggcta tttcaaattc gcgcccctgc agtcggaaat cggcgaaaag ctgctggcgg
     2641 aaaacggcat cgacaaaatc gaaaccgatt cggtcgtgct gattgaagac ggcaaagttt
     2701 acacgcattc gaccgccgcg ctgcgcgtcg cgcgcaaatt ggacggcgcg tggaaattca
     2761 cttacggctt tgtcgtcgtg ccgcgcttcg tgcgcaattt tttctataga ttgtttgcca
     2821 aatatcgcta taaaatgttc ggcaaacagg acgcctgtat gctgccgacg ccggaaatcc
     2881 gccaaaggtt tctggcgatg agctaaagcg gctggatatt tgccaaaacc cgatttttgc
     2941 ttgtgaatct tcacctgtca cgtgtgaatc ttcatctgtc ggctgtgaat gttcgccgtt
     3001 cacttgtgaa tatgcatctg tcggttgtga atgtgcatct gtcacttgtg aatgttcaag
     3061 tgtcacgtgt gaatcttcat ctgtcacgtg tgaatcttca tctgtcactt gtgaatgttc
     3121 atctgtcagt tgtgaatgtg catctgtcag ttgtgaatgt tcatcgttca cgtgtgaatc
     3181 tgtgccggtc agttgtgaat gttcgtcggt cagttgtgaa tctgcgccgg tcacgtgtga
     3241 atgttcatcg ttcacgtgtg aatgttcgtc ggtcacttgt gaatcttcat cgttcacttg
     3301 tgaatgagcg ttcgtcagtt gtgaatctgc ggtcagcggt tgtgaatgct cgatttcaca
     3361 tcgccggaag acgttttttg gttcgagcga taagattttg gcgtaaaaag gaatttttta
     3421 taatgaaagc aatctggaac ggcgcggttt tagccgaaag cgacgagaca atcgtcgtcg
     3481 aaggaaacca ttattttccc gaaagttcga taaacaagga atattttcag ccttcgcaaa
     3541 cgcacacggt ttgcggctgg aaaggaacgg cgagctatta cgacgtcgtc gccaatggcg
     3601 cgacgaacaa ggacgccgcg tggttttatc ccgacgcgaa gacggaagcg aaagaaattg
     3661 aaaattacgt cgcgttctgg aaaggtgttg aagttaaggc ttaaaaatac tccaaactac
     3721 ataagtagat atgctcaaga aaatattaaa cttgttggag ataaatatga atcaaccgga
     3781 aatttccttt tccggttcga cgctgaccgt cgaaaatcgc tcctggcaag tcgaatatcc
     3841 tattcatcaa gcttttgcct tgaa
//
LOCUS       DASWNE010000085         2022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_144097, whole genome shotgun sequence.
ACCESSION   DASWNE010000085 DASWNE010000000
VERSION     DASWNE010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144097"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..668
                     /locus_tag="VGB68_01755"
     CDS             138..668
                     /locus_tag="VGB68_01755"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HEY0047981.1"
                     /translation="MKKIALMFFVLALAAVVVPAQKERTMTIKVYFGNEKSNPNMQDC
                     GKVEATTRVIPKTKTTARAALEELFKGVTPEEEAKGYTSLSAAETKGILKSVRIKNGA
                     AYVNFNPVVYRQTGTATTSCGGSYFFSSVEKTLRQFPTIKKVFYAIDGSPKDFYDWVQ
                     VGECPKELKNCSNKDF"
     gene            756..1073
                     /locus_tag="VGB68_01760"
     CDS             756..1073
                     /locus_tag="VGB68_01760"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0047982.1"
                     /translation="MSNNNFPKLSAKEMLILEMLIGKGEMFGLEMVEASSGNLKRGTI
                     YVTLQRMGDKGYIESREEPRSMPEIGIPRRKYLATGLGERVFQANVKALEFFNSDFAL
                     GGL"
     gene            1075..1587
                     /locus_tag="VGB68_01765"
     CDS             1075..1587
                     /locus_tag="VGB68_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047983.1"
                     /translation="MQSNLKGNIEIWSVRFGILSLLGCFLFYFSLKLGDFSTSVSRGF
                     LFAVMLAAIPLFISVFALFGMFIGSVIVDLIKFIERNLPAVKKTVSSLFSSKLLKITG
                     FLYSPKTQREIFEPIAADWQEEYFEALSKKEVWKARWINVRYTYAFLGAMWQKSPFGD
                     LIEFISKIAK"
     gene            1601..1813
                     /locus_tag="VGB68_01770"
     CDS             1601..1813
                     /locus_tag="VGB68_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047984.1"
                     /translation="MSVSTSKMDKTIVRKGKLKEQGNDFEFWQTQSYEARLATVEQIR
                     QEYNSWKYGSEQGFQRVYRVIKRAEK"
BASE COUNT          612 a          409 c          477 g          524 t
ORIGIN      
        1 ggaaattcga ttcgatggca gacgaagagc tttaagctcg atttcaggcg taaaagccgc
       61 gccgagcggg cggcgaaaat tcagccgcag ccgacgcaag tcgagccgaa attcctgctg
      121 ataaattaag gatgaaaatg aaaaaaattg ctttgatgtt ttttgttctg gcgcttgccg
      181 ccgtcgtcgt tccggcgcaa aaagagcgga cgatgacgat aaaagtttat ttcggcaacg
      241 aaaaatccaa tccgaatatg caggactgcg gcaaagtcga ggcgacgacg cgcgtgattc
      301 cgaaaacaaa gacgacagcc agagccgcgc tcgaagagct ttttaaaggc gtcacgcccg
      361 aagaagaagc caaaggctac acttcgctgt cggcggcgga aacaaaggga attttaaaaa
      421 gcgtcaggat aaaaaacgga gccgcgtacg tcaattttaa cccggtcgtc taccggcaaa
      481 ccggcacggc gacaaccagc tgcggcggca gttacttttt ctccagcgtc gaaaaaacct
      541 tgcggcaatt tccgacgatt aaaaaagttt tctacgccat cgacggcagc ccgaaagatt
      601 tttacgattg ggtgcaggtc ggcgaatgcc cgaaggaact gaaaaattgc agcaacaagg
      661 atttttaaaa caggcgatta aaaaaatgtt gacaaagagt tgattcttgc ttacagtaag
      721 aatcaactct ttctttatgt aagacaggat aaaatatgag taataataat tttccgaaac
      781 tttcggcgaa agaaatgctg attctggaaa tgctgatcgg caaaggcgaa atgttcgggc
      841 tggaaatggt cgaagcctca agcggcaacc tgaaacgcgg aacgatttac gtcacgcttc
      901 aacgaatggg cgacaaaggc tatatcgaat cgcgcgaaga accgcgctca atgccggaaa
      961 tcggaatccc gcgccgcaaa tatttggcga caggtctcgg cgaaagagtt tttcaagcga
     1021 atgtcaaagc gcttgagttt ttcaattccg attttgcttt gggaggttta tgatatgcaa
     1081 tctaatctca aaggaaatat agagatttgg agcgtgcgat ttggaatcct ttctttgctg
     1141 gggtgttttc tattttattt ctcacttaag ttaggcgatt tctcgacatc cgtaagcaga
     1201 ggatttttat tcgcagtcat gcttgccgct ataccacttt ttatatcagt ttttgcttta
     1261 ttcggaatgt tcattggtag tgtcatagta gatttaatta agtttatcga aagaaattta
     1321 cctgccgtaa aaaagacagt ttcttctctt ttttcctcaa agctgcttaa gattacgggt
     1381 tttctatatt cacctaaaac acaaagagaa atttttgagc cgattgccgc cgattggcaa
     1441 gaggaatatt ttgaagcatt aagcaaaaaa gaagtttgga aggcgcggtg gataaatgtg
     1501 cgttacactt atgcttttct cggagcaatg tggcagaaga gtccgtttgg cgatttgatt
     1561 gaatttataa gcaaaattgc gaaataataa aataagattt atgagcgttt cgacatcgaa
     1621 aatggataaa acaattgttc gcaaaggcaa gttgaaagaa cagggaaacg atttcgagtt
     1681 ctggcaaact cagtcttatg aagctcgttt ggcgacagtc gaacaaatca gacaggaata
     1741 caacagttgg aaatatggtt ctgaacaagg atttcagaga gtttatcgcg tcattaaacg
     1801 cgctgaaaaa taaacgtcag ccgcaaaaaa aagcaaacgt ttccaatctt aagcccaaca
     1861 aggattttta attctgcggc agataatcct tcaaatatct gcccgtgtgc gaatctgcgc
     1921 ttttcacaat ttcttcaggc gttcccgtgg cgacgatttc gccgccgccg atgccgcctt
     1981 cgggtcctaa atctatgacg taatcagccg tttttatcac gt
//
LOCUS       DASWNE010000086         5161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_214456, whole genome shotgun sequence.
ACCESSION   DASWNE010000086 DASWNE010000000
VERSION     DASWNE010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5161
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214456"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2085)
                     /locus_tag="VGB68_01775"
     CDS             complement(<1..2085)
                     /locus_tag="VGB68_01775"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEY0047985.1"
                     /translation="MHRLKLFVSLLFILLAFTFFGQHRFKTSSAVRNPAGGAALSAPS
                     GVSASDGDYADKVGVMWNTVRGATLYRIFRSAGSEPSSAAEVGTTAANYFFDTTAQAN
                     QNYFYWVRAENTGSSSSLSSSEQGSRAAGSFQSEIFSPLSPPPAPNGNPVTAAKASLG
                     KALFWDEQLSSTLTVSCGTCHRPDKGGSDARTVVNNARSRNPGFDNIFNTPDDVFGSP
                     GVPQNNLDGTYSPSSSFGFNEQVTPRKAPSYLNAGLAFNGSFWDGRASNTFRDPLSNQ
                     VLLTEWASLESQILSPPVSDSEMGHANRNWTQVANQITAAKPLALAVDVPPSLAEWID
                     GRTYTELFEEAFGTPEVTPSRIAMAIATHERTLFTDRTPLDRYASAIEPLTAQEDRGR
                     EIYVTAQCNFCHGGPLLSDQNFHNVGVRPQVEDLGRAIVTGNVNDRARFKTPTLRNVE
                     LRAPYMHNGRFGTLEEVVEFYNRGGDFNAPNKDPRVRPLNLSGAQRADLVAFLKRPMT
                     DARVRGELPPFDRPQLYTESNRVPVITGTGRAGSGDITPQAIAIEPPLVGNPSFTVAV
                     SRALGGAQAVLVIDSNDPGVGEAIPAAGSFARVAVNISGSGAGNGFGSVSLPIPDDPA
                     LTGRTFYGRWYVADASAASGFSVTQAFRFTVFGDSSPTTNKTDFDFDGDGRADLSVFR
                     PSNGTWYISNSSN"
     gene            complement(2422..3963)
                     /locus_tag="VGB68_01780"
     CDS             complement(2422..3963)
                     /locus_tag="VGB68_01780"
                     /inference="COORDINATES: protein motif:HMM:NF024972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047986.1"
                     /translation="MNTKKTRAFAAGENPTPVRFASTRRMIVIAAFCLVSSAFSAGIA
                     SAQTTDFYQTGQIRAVVSERETEIPSWKGTSVDAGFKFSGVARSKVDFYFTYSAFEDG
                     VNTEKWVALPGDRNWSELSNQPVRVSGRLKGNKLSNAEATPLARLPEDSRTVVPATVG
                     RYKVVAVPLTIQQPTAQGKPLQSSAALSVTPEAIRNTLFNAPNSVNKFYLEASYGMFG
                     WTGVHHPQADVVPVTIQATISGNCQEQIMNQFTATVRQRLLEQNIDTTNGSVDLGIII
                     FNDMPGCPNYPFATRGALGARGVPQWLWMPESWFATGPSITAHEIGHALGGNHPLALR
                     CADFDNSQTCVVVEAADRDLMTYGGEFYLMPNNYERRRWGWHPAGVFDNPPIGSVRLF
                     DLQSPVLPFVKDSARRGRFYFRNLTTGPFVEHDIYPEARRNWGQFERYHGINESFRAG
                     ITLRIGHVNYGAPEVVSVLIDTNGTSRLEDAPLRENQQLSIGGIVIKCIAEHNASRGT
                     RMRAE"
     gene            4822..>5161
                     /locus_tag="VGB68_01785"
     CDS             4822..>5161
                     /locus_tag="VGB68_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047987.1"
                     /translation="MQVNLHEHTFELSAHTNDTLNEAKNLHPVLSMVSDETAKSYTHF
                     ADIPDEHQKGDALRWVQVVRLQQPGSHLNEVVLMNLVIPPDILRAFYKSPMWKQRHTR
                     RYGRHKSPAIP"
BASE COUNT         1209 a         1363 c         1338 g         1251 t
ORIGIN      
        1 gtttgagctg ttgctgatat accaggtccc gtttgaaggg cgaaaaacgg aaaggtcggc
       61 gcgcccgtcg ccgtcgaaat caaaatctgt tttattggtt gtcggcgacg aatcgccgaa
      121 gacggtaaaa cgaaacgcct gtgtgaccga aaatccgctt gccgcgctcg cgtcggcgac
      181 ataccagcgc ccgtaaaaag ttcgacctgt caaagccgga tcgtccggaa tcggcaggct
      241 gaccgaaccg aaaccgtttc ccgccccgct gccggagata ttgaccgcga cgcgcgcgaa
      301 cgagccggcg gcgggaatcg cttcgccgac gcccggatca tttgaatcaa tcaccagaac
      361 ggcttgcgcc ccgcccaggg cgcgcgatac ggcaacggta aagctcggat ttccgacgag
      421 cggcggttcg atggcgatgg cttgcggcgt gatgtcgccc gagccggcgc gcccggttcc
      481 ggttatgacc ggaacgcgat ttgattcggt ataaagctgc gggcggtcaa acggcggcag
      541 ctcgccgcgc acgcgcgcat ccgtcatcgg tcgctttaaa aaggcgacga ggtcggctct
      601 ttgcgcgccc gacagattaa gcgggcgaac gcgcgggtct ttgttcggcg cgttgaaatc
      661 gccgccgcga ttataaaact cgacgacctc ttcgagcgtt ccaaacctgc cgttatgcat
      721 atagggcgcg cgcagctcga cgtttctcaa ggtcggagtt ttaaatcggg cgcggtcatt
      781 gacgtttccg gtcacaattg cccgtccgag gtcttcgacc tgcggtcgga cgccgacgtt
      841 atgaaaattt tgatccgaga gcagcggacc gccgtgacag aaattgcatt gggcggtgac
      901 gtaaatctcg cgtcccctgt cttcctgagc cgtgagcggc tcgatcgccg aagcataccg
      961 gtcgagcgga gtgcggtcgg taaataaagt ccgttcgtgc gtggcgattg ccatcgcaat
     1021 ccgcgacggc gtaacttccg gcgttccgaa agcttcctca aacaattccg tataagtccg
     1081 cccgtcaatc cattcggcga gcgacggcgg aacgtcgacg gcgagcgcca gcggtttcgc
     1141 cgcggtgatt tgatttgcca cctgcgtcca gtttcgattg gcgtgtccca tttcggaatc
     1201 ggaaaccgga ggacttaaaa tctggctttc gagcgatgcc cattccgtca gcagaacctg
     1261 attcgagagc gggtctcgaa aagtattcga cgcgcgtccg tcccagaatg agccgttaaa
     1321 cgccagaccg gcatttaaat acgaaggcgc ttttcgcggc gttacctgtt cattaaatcc
     1381 gaaactgctt gacggcgaat aagttccgtc caaattgttt tgcgggacgc ccggcgagcc
     1441 gaaaacgtcg tcgggcgtgt tgaagatatt gtcaaagccc ggatttctcg agcgcgcgtt
     1501 attgacgaca gttcgcgcat cggaaccgcc tttgtccggt cggtgacacg tcccgcagga
     1561 aaccgtcaaa gtcgaagaaa gctgctcgtc ccagaagaga gctttgccga gagatgcttt
     1621 ggctgccgtg accgggtttc cgttgggcgc gggcggcgga gataacggag aaaaaatttc
     1681 tgattgaaag cttccggcgg cgcgcgagcc ctgctcggag ctgctcaaac tgctgcttga
     1741 gccggtgttt tccgcgcgca cccagtaaaa atagttttga ttggcttgag ccgtcgtatc
     1801 gaaaaaataa ttggcggcgg tcgttccgac ttccgccgcg gacgacggct cgctgccggc
     1861 gctgcgaaaa atccggtaaa gcgtcgcgcc gcgcacggtg ttccacatca cgccgacttt
     1921 atcggcataa tctccgtccg atgccgaaac tccgctcggg gcggaaagcg ccgcgccccc
     1981 ggcaggattt ctgacggctg aagacgtctt aaatcgatgc tgaccgaaaa aggtaaacgc
     2041 cagcaaaata aataataaag atacgaacag ttttaagcga tgcattcgat taatgttccc
     2101 tgaccgacta tttccctgtg aattaatttc tgaaaacgaa tttgtaaatg cctgccggcg
     2161 tccgcgaaat ttattcacgg gtcggcgttt tattcaatca acaagcctgc gaaaatccgt
     2221 cggcgcagag cttaaattaa ggtgtgtgcg gagtaagaaa tgagtgaaaa caattcttct
     2281 gatacaaaca ggtatcggct aaaaaaattt tattgtaagg attttgctct cgctcataaa
     2341 cattttaact ttattgtctt caattgacga gccggcaaca ggaaaagctt caatcgaatt
     2401 ttacaaaaaa gccttttgcc gtcattccgc tctcatccgc gttccgcggc tcgcgttatg
     2461 ctccgcgatg catttgatga cgatcccgcc aatcgaaagc tgctggtttt cccggagcgg
     2521 cgcgtcctcc aaccgggaag tgccgttagt atcaatcaat accgatacca cctccggcgc
     2581 gccgtagttt acgtgaccaa tcctgagcgt aatccccgcg cgaaaagatt catttattcc
     2641 atggtatctc tcgaattgcc cccagtttcg gcgcgcttcc ggatagatgt cgtgttcgac
     2701 gaaaggtccg gtcgtcaaat ttcggaaata aaatcttccc cgccgcgcgc tgtctttaac
     2761 gaatggaagg acgggcgact gcaaatcaaa caaccgaaca gacccaatcg gaggattatc
     2821 gaatacgccc gccggatgcc agccccagcg gcgtctctcg taattattcg gcatcaggta
     2881 aaattcgccg ccgtatgtca ttaaatctct gtcggcagcc tcgacgacca cgcaggtttg
     2941 cgaattatca aaatcggcgc aacgcaatgc gagcggatgg ttgccgccga gagcgtgccc
     3001 tatttcgtgc gccgttattg aaggaccggt tgcaaaccag ctttccggca tccataacca
     3061 ctgcggcacg ccgcgcgcgc cgagcgcgcc gcgcgtcgcg aaaggatagt tgggacagcc
     3121 cggcatatcg ttgaaaatta taattccgag gtcgacgctg ccgttcgtcg tatcgatgtt
     3181 ttgctcgagc agccgctgcc ggacggttgc tgtaaattga ttcataattt gctcctggca
     3241 gttgcctgaa atcgtcgctt gtatggttac cggcacgaca tctgcctgcg gatgatggac
     3301 gccagtccac ccgaacatcc cgtaggaggc ttccagataa aacttattga ccgagttcgg
     3361 agcattaaac agtgtgttgc gaatcgcttc cggcgtcacg cttaaagcgg cagagctttg
     3421 aagcggcttc ccctgcgccg tcggctgctg gatagtcaaa ggcacggcaa cgactttgta
     3481 acgaccgacg gtcgccggaa cgacagtccg ggaatcttcg ggcaatcgag ccagcggcgt
     3541 cgcctctgcg tttgataatt tattcccctt cagcctgccg ctcactcgaa ccggttgatt
     3601 cgaaagctcg ctccaatttc tatcgccggg aagggcgacc catttttcag tatttacacc
     3661 gtcttcgaaa gctgaatagg taaaataaaa atccactttc gacctggcga cgcccgaaaa
     3721 tttaaacccg gcgtcaacgg aagtgccttt ccaggacggt atttccgtct ctctctccga
     3781 gacgaccgct ctgatttgtc ccgtttgata aaaatcggtg gtttgcgctg aagcgattcc
     3841 ggcgctgaag gcagacgaaa ccaaacaaaa tgccgcaatt acaatcatcc ggcgcgtcga
     3901 cgcaaatctg accggcgtcg gattttcccc ggcggcaaac gcgcgagttt tttttgtatt
     3961 catttcgttt tatccttcgc ctttattttg ttttatcctc tgcctttccg gatgcgtatg
     4021 accgctctga ggaattttcc ctgtcttgtg taattagata tataccatca acgctcgtaa
     4081 tttacgagct aaatcccgcc cgtgccggaa caattataaa ccggcaggct ttgaatttgg
     4141 ctgtgtgagc tcgatatccg atacgcgaaa aagctgttgc tccaaacatc cgttaaggag
     4201 atgcggcgta caatgcgcaa agccgttgag cgtttttgaa attaattttg aaattaattt
     4261 cgacatatca gtaatattca gcttcggaag tcggaaggct gaatattact aaactaatag
     4321 acgttatcag acccataaag aagccgaccg cgttttatta tcagtgctcg cggaacaatg
     4381 tttgactttt tactttcccg atgtcaaaac cgggatgtcg ggcgctggaa taccgggagc
     4441 gccggagcgt cgagcgcgcg agccgcgaaa aaagcaaaag cggaataaat aaaattccga
     4501 aattaacagg ctttgatgat tttatttatt attgcgtttt gtcgataaat ggaaaaatga
     4561 taatgtaaag accttgtttt tttctctgga aaaaaccagc tttttcgtgc tacttccaac
     4621 tatgggcttt tttttcgcct aaagtctgtt atctcgacaa cattttctta atcaaaattt
     4681 tgcctggcgg cagaagtgtc tgaaatcaac actgctaaaa cgttttacgt tcttttttag
     4741 cctaactttt attttctgaa ggagatctca atttattccc gttttcattt cgcattaggt
     4801 cacttagatg attccgattc attgcaggta aatctgcacg aacatacttt tgaactctcg
     4861 gcgcacacga atgatacttt gaacgaagcg aaaaatcttc atcctgtttt gtcgatggta
     4921 tccgacgaaa ccgctaaaag ctacacacat ttcgcagata ttccggacga gcatcagaag
     4981 ggcgacgcgc tgcgctgggt tcaggtcgtc agactgcagc agccgggctc tcatttgaat
     5041 gaggtcgtgt tgatgaatct ggttatcccg ccggatattc tgcgcgcttt ttacaaatct
     5101 ccgatgtgga agcagcgtca tacgcgccgt tacggtcggc ataaatctcc ggcaattccc
     5161 g
//
LOCUS       DASWNE010000087         3862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_250434, whole genome shotgun sequence.
ACCESSION   DASWNE010000087 DASWNE010000000
VERSION     DASWNE010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250434"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="VGB68_01790"
     CDS             complement(<1..981)
                     /locus_tag="VGB68_01790"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEY0047988.1"
                     /translation="MKPQDLIRKKRDGGRFLPEEINAFITGVCDESWADYQISAFVMA
                     CFINGLRQTEQNALVEAMLKSGETLDFSDIDAPLADKHSTGGVGDKTSLLIAPLVASC
                     GVAVPMISGRGLGHTGGTLDKLESIAGYNVNLSTAEFKEIIKKCGFAMAGQTADIVPA
                     DRKLYALRDATATVESIPLIVASIMSKKLAEGLDALVLDVKTGSGAFMQEETEARKLA
                     KALVKTGNAFGVRTEAILTDMNQPLGKYVGNALEVYECIKLLQNEGEEQSRQTLELSI
                     ELATRMLVLTKTEKTLKAAKEKIQNALASGAALEKFRENITLQGGDAKICD"
     gene            1084..1602
                     /locus_tag="VGB68_01795"
     CDS             1084..1602
                     /locus_tag="VGB68_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047989.1"
                     /translation="MRSKALLSIIAFLAAFGISVAVTPRAPKSTVSPYVKRGCSQSES
                     ARKITNLLSQDIENGRIRDRRIDGFSDYGLSREAYFNGFATTTKEYADASASIDDSDL
                     PRDFKTAWREHMRAWEKHSDFLEAVGDSSKAPGSSFSRAFSEQNKEISETWFEVLRIA
                     RKYDAYIPAGAY"
     gene            complement(1599..2081)
                     /gene="ispF"
                     /locus_tag="VGB68_01800"
     CDS             complement(1599..2081)
                     /gene="ispF"
                     /locus_tag="VGB68_01800"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEY0047990.1"
                     /translation="MFRIGLGNDIHRLTEGKPLILGGVEIESDKGAEGHSDADALLHA
                     VTDAILGALALGDIGSHFSDRDERWRNADSTVFLRRAFGLMKEKGFRIVNLDSTISLE
                     KPKLRPHIDAMRGNLARILEVDVDSVSVKAKTGEKVDAVGENRAIKAEVIILLAENRV
                     "
     gene            complement(2084..2767)
                     /gene="ispD"
                     /locus_tag="VGB68_01805"
     CDS             complement(2084..2767)
                     /gene="ispD"
                     /locus_tag="VGB68_01805"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEY0047991.1"
                     /translation="MNTAIIVAAGSGKRFGGKTPKQFLEVLGKPLIIHTIQRFEACRE
                     IDEIILVLASTETAKFLQIAGKYNLKKISKVVAGGSARAESVRKGLSAIRAATAEIVA
                     VHDGARPLVSIEEIAQTVKKARETGAACLVSAVTDTIKEIKDGKIIRTIDRSTLRRAL
                     TPQCFRYEILHRAFENADLSAAATDECFLVEKLGQEIAVVEGSARNIKITHAEDLTLA
                     ESLLKQTTL"
     gene            complement(2841..>3862)
                     /locus_tag="VGB68_01810"
     CDS             complement(2841..>3862)
                     /locus_tag="VGB68_01810"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HEY0047992.1"
                     /translation="VIVAAFVIAFEMRARKASLKTLIGAAIGSILGIIGAYLIGMLIS
                     SQDINTVPGEMKVFLTIALAFFMGYMGLMVGAAKGEYIDLSVLGGLFSDKNVRRDYKI
                     LDTSVIIDGRIADVAETGFLGGTLVIPQFVLRELQQVADSPDSSKRQRGRRGLDMLQR
                     LRNNSKLDIQIVETDFPAVKEVDLKLIELGKQLEAVIITNDFNLNKVSQLRGVSVLNV
                     NELANALKPVVLPGEAMRVFVLKEGKEYNQGVAYLDDGTMVVIDNARKLIGKNADIAV
                     TSVLQTTAGKMIFGRLWEEKDEVGGGEVSNQNIHDSRTAGFRKQYRDMRHTSAMPIRM
                     EEEIE"
BASE COUNT          839 a         1065 c          974 g          984 t
ORIGIN      
        1 gtcgcagatt ttcgcgtcgc cgccttgcag tgtaatgttt tcgcggaatt tttccaatgc
       61 cgcgccgctc gcgagcgcgt tttgaatttt ttcctttgcc gctttcagag ttttttccgt
      121 tttggtcaaa accagcattc gcgtcgccag ctcgattgac agttcgagcg tctgccggct
      181 ttgctcttcg ccttcgtttt gcagcagttt gatgcattcg taaacttcca gcgcgttacc
      241 gacatatttt ccgagcggct gattcatatc ggtcaaaatc gcttcggtgc ggacgccgaa
      301 agcgtttccc gttttgacca gcgctttcgc cagtttgcgc gcttcggttt cttcctgcat
      361 aaacgcgccg ctcccggttt tgacgtccag aacgagcgcg tccaagcctt ccgccaattt
      421 tttcgacata atcgaagcga cgattaaagg aatcgattcg accgtcgccg tcgcgtcgcg
      481 cagggcgtaa agcttccggt ctgccggaac gatgtcggcg gtttgtcccg ccatcgcaaa
      541 accgcatttt ttgatgattt ccttaaattc ggcagtcgaa agattgacgt tgtagcccgc
      601 gattgattcg agcttgtcga gcgttccgcc cgtgtgcccg agtccgcgac cggaaatcat
      661 cggcacggcg acgccgcaag acgcgaccag aggcgcgatt aacaaggaag ttttgtcgcc
      721 gacgccgccc gtcgaatgct tgtcggcgag cggcgcgtcg atgtccgaaa aatcgagcgt
      781 ttcgccggat ttgagcatcg cttcgaccag cgcgttttgc tcggtctggc gcaagccatt
      841 gataaaacaa gccatcacga aggcggaaat ttgataatcc gcccagcttt cgtcgcagac
      901 gccggtgata aaagcgttga tttcttcggg cagaaatctt ccgccgtcac gttttttgcg
      961 aattaaatcc tgtggtttca tttacgggaa tactttagca gaattaccgg cgcgaataaa
     1021 tccgacaaat ttttcagaaa gatatttacc ttttgccgcg caatgagcga taataaaatt
     1081 gttatgagaa gtaaagcatt attatcaatc atcgcatttc tcgcggcatt cggaatttcg
     1141 gtcgccgtca ctccccgcgc accgaaatca accgtctcgc cgtacgtcaa aagaggctgc
     1201 tcccaatcag agagcgcgcg caaaataacg aatctgcttt cgcaggacat tgaaaacgga
     1261 cgcatcagag accgccgaat agacggtttc agcgattacg gattgtcgcg cgaagcctat
     1321 tttaacggtt tcgcaacgac taccaaagaa tacgcggacg cttcggcgag catcgacgat
     1381 tcggatttgc cgcgcgattt caaaaccgcc tggcgcgagc atatgagagc ttgggaaaaa
     1441 cattcagact ttctcgaagc cgtcggcgat tcttcaaaag ctcccggcag cagtttttcc
     1501 agagcatttt cggagcagaa taaagaaatt tcggaaacct ggttcgaagt tctgcgcatc
     1561 gctcgaaaat acgacgctta cattccggcg ggcgcgtatt aaactcgatt ttccgcgagt
     1621 aaaattatta cttccgcttt gatggcgcgg ttttctccga ccgcgtcaac tttttctccg
     1681 gttttcgctt tcacgctcac cgaatcgaca tcgacttcca gaatccgcgc taaattccct
     1741 cgcatcgcgt cgatgtgcgg acgcagcttc ggcttttcca ggctaatcgt cgaatccagg
     1801 ttgacgatgc gaaatccttt ttccttcatc aagccgaacg cgcggcgcaa aaaaaccgtg
     1861 ctgtcggcgt ttcgccagcg ctcgtcgcgg tcggaaaaat gcgagccgat gtcgcccaga
     1921 gccagcgcgc ccagaatcgc gtccgtcacg gcgtgcaaaa gcgcgtcggc gtcggaatgt
     1981 ccttcggcgc ctttatcgga ttcaatctcc acgccgccca aaatcaaagg cttgccttct
     2041 gtcagtcgat gaatatcatt tcccagcccg atacgaaaca tttttataat gttgtttgtt
     2101 ttagcagact ttccgccaaa gttaaatctt ccgcgtgcgt gattttgata tttcgcgcgc
     2161 tgccttcgac gacggcaatt tcctgcccga gtttttcgac cagaaaacat tcgtccgtcg
     2221 ccgccgcact caaatcggcg ttttcaaacg cgcggtgcag aatttcataa cgaaaacatt
     2281 gcggcgtcaa agcgcgccgg agcgtcgagc ggtcaatcgt ccggatgatt ttgccgtctt
     2341 tgatttcctt aatcgtgtcc gtgacggcgg aaaccagaca agccgcgccg gtttcccggg
     2401 cttttttgac ggtctgcgcg atttcttcaa tcgaaaccag cgggcgcgcg ccgtcgtgaa
     2461 cggcgacgat ttcagccgtc gccgcgcgaa tcgcgctcaa gccttttcgg acggattcgg
     2521 cgcgcgcgct tccgcccgcg accactttcg atattttttt taaattgtat tttccggcga
     2581 tttgcagaaa ttttgcggtt tcggtcgaag cgagaacgag aatgatttcg tctatttcgc
     2641 ggcaggcttc aaaacgctga atggtatgaa taataagcgg ttttccgaga acttccagaa
     2701 attgtttcgg ggttttcccg ccgaaacgct ttccgcttcc ggcggcgacg atgattgctg
     2761 tattcattcg agtgctgagt gctcagcgct gagtgctgag cattgatttc gactcagcac
     2821 tcagcactta gcactcagca ctattctatt tcttcttcca tccgaatcgg catcgcgctg
     2881 gtgtggcgca tatcgcggta ttgtttgcgg aaaccggcgg tgcgcgaatc gtgaatgttc
     2941 tgattgctga cctcgccgcc gccgacttca tctttttctt cccacaggcg accgaaaatc
     3001 atttttccgg cggtcgtctg caaaacgctg gtgacggcga tgtccgcgtt ttttccgatc
     3061 agcttgcgcg cgttgtcgat gacgaccatc gtcccgtcgt ccagataagc gacgccctga
     3121 ttgtattcct tgccctcttt caaaacaaaa acgcgcatcg cctcgcccgg cagaacgacc
     3181 ggctttaagg cgttggcgag ctcgttgacg ttcagaacgc tgacgccgcg cagctgcgat
     3241 actttgttta aattaaaatc gttcgtgata atgacggctt cgagctgttt gccgagttcg
     3301 ataagcttta aatcgacttc tttaacggcg ggaaaatcgg tttcgacaat ttgaatgtcg
     3361 agcttcgaat tattgcgaag tcgctgcagc atatcgagcc cgcgccgccc gcgctggcgt
     3421 tttgacgaat cgggcgaatc ggcgacctgc tgcaattcgc gcagaacgaa ttgcgggatg
     3481 accagcgttc cgcctaagaa tccggtttcg gcgacgtccg cgatacgccc gtcgataatg
     3541 acgctggtgt ccagaatttt gtaatcgcgg cgcacgtttt tatcgctgaa aagcccgccc
     3601 aaaaccgata aatcaatgta ttcgcctttc gccgcgccga ccatcaaacc catatagccc
     3661 ataaaaaagg cgagcgcgat ggtcagaaaa accttcatct cgccgggcac ggtgtttata
     3721 tcctgcgagc tgataagcat tccgattaaa taagcgccga tgatgcctaa aatcgagccg
     3781 attgccgcgc caatcaaggt tttgagcgag gcttttcgcg cccgcatctc gaaagcgatg
     3841 acgaaagccg cgacgatgac gc
//
LOCUS       DASWNE010000088         6517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_462472, whole genome shotgun sequence.
ACCESSION   DASWNE010000088 DASWNE010000000
VERSION     DASWNE010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6517
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462472"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..4062
                     /gene="rpoC"
                     /locus_tag="VGB68_01815"
                     /pseudo
     CDS             10..4062
                     /gene="rpoC"
                     /locus_tag="VGB68_01815"
                     /inference="COORDINATES: protein motif:HMM:NF016858.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
     gene            4261..5229
                     /gene="bla"
                     /locus_tag="VGB68_01820"
     CDS             4261..5229
                     /gene="bla"
                     /locus_tag="VGB68_01820"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF012099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase, subclass A2"
                     /protein_id="HEY0047993.1"
                     /translation="MKKMLFRNLYLFLFTIIYLVFINGCNEIKKENPVSAPTKAAAPD
                     VKNEPKSALHKRIEEIAGNAQGKVGVAALVLETGESFGLNENQHFPMQSVYKFPIAMA
                     VLKRVEQGNLKLEQKIRIEKSDLVPKRLRSPIRDRYPQGAELGLAEVLKYSVSDSDGT
                     ACDVLLKAVGGADAVTKYLREIGVGDMVVATTEKEMAQDESVQYQNWSSPKAANDLLR
                     LLHEGRTLSENSRALLLQWMTETPTGAKRIKGLLPAGTVVAHKTGTSGTFDGLTRATN
                     DVGLIALPDGRHLSISIFVSDSKADEKTREEVIAKITRAVWDEFVK"
     gene            complement(5293..>6517)
                     /locus_tag="VGB68_01825"
     CDS             complement(5293..>6517)
                     /locus_tag="VGB68_01825"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEY0047994.1"
                     /translation="MTARGVPAVTEINLGQPVFFKEVNLMLREVSLEDWKTYLRWMTI
                     NAAAPTLSKAFADENFNFYSRYLSGTKEQQPRWKTCVNAVDNHLGEALGQEYVKKAFT
                     PAARARMNEMIDNLLAAMKDRVNKLDWMSAETKKQAQAKLATFKRKIGSPDKLRGYNG
                     LSVKRDSYSANVLRSGQFQVRRNLLDINQPVDRSRWGFTPPTVNASYSGVNNDITFPA
                     GILQPPFFNPAADDAINYGAIGGVIGHEISHGFDDSGSRFDAEGNLKMWWTKEDRAKF
                     EERAACVVKQFGEYEVQPGLFINGKLTLGENIGDLAGLTVAYDAYKKSLEGKPRPSEI
                     DGFTPEQRFFLGWAQVWAGKYTPEAEILQVKTNTHSLPRWRVNGPLSNMPQFAQAFGC
                     KSGDKMVRTDACLIW"
BASE COUNT         1613 a         1668 c         1863 g         1373 t
ORIGIN      
        1 ctcggtcata tcgagcttgc cagcccgtgc tcgcacgtat ggtttttcaa aggcttgccg
       61 tcgcgtatcg gtcatctgct ggatataacc ttgcgcgacc tcgaaaaaat tctttatttc
      121 gagctgtaca tcacggttga cccgggcgaa gttcccggat taaaagaaaa agatttgctg
      181 accgacgagc gttaccgcga attgacgcgc gattatccca accagtttgt ggcgaaaatg
      241 ggagccgaag ccatcaaaga gcttttgatg aaggtcgacg tcgccgagct ggttacggat
      301 ttgcgcggaa aaatgcgcga agaaacctcg cagcagaaaa aactgaaata ttccaagcgt
      361 ctgaaagtgg cgaactcgtt tatgcgctcg cacaacaatc cggaatggat gattttggac
      421 gtgattccgg tgattccgcc ggagcttcgc ccgctggtgc cgctggacgg cggcaggttt
      481 gcgacttcgg atttaaacga ttagtatcgc cgcgtcatca atcgcaacaa ccgtttgaaa
      541 aagcttatcg aactgcgcgc gccggaagtt atcgtccgca acgaaaaacg tatgctgcag
      601 gaagcggtcg acgctctgtt cgacaacggt cgtcgcggtc gggtcttgcg cggcgcgaac
      661 aatcgtcctt tgaaatccct gtcgggaacg ctcaaaggaa aacagggacg cttccgccag
      721 aatctgctcg gcaaacgcgt cgattattcg ggacgctcgg tgatcgtcgt cggaccggaa
      781 ctgaagctgc accagtgcgg tttgccgaaa aaaatggctc tggaactgtt caagccgttt
      841 atttataaca agctggaaaa agaagaatac gcggcgacga ttaaacaggc gcgtgagatg
      901 gtcgagcgcc aggagccggt cgtttgggac attctcgaag aagtcatcaa acagcacccg
      961 gttttgctga accgcgcgcc gacgcttcac cgactcggaa ttcaggcttt cgagccggtt
     1021 ctggtcgaag gtaaagcgat taaaattcac ccgctcgtct gcacggcgtt taacgccgac
     1081 tttgacggcg accagatggc ggttcacatt ccgctctcgc ccgaagcgca gattgaagcc
     1141 agcgttttga tgctcgcttc aaacaatctg ctttcgcccg cgtccggaca gccgattacg
     1201 gttccgtcgc aggacatcgt cttgggttgt tactacctga cgaaagaccg tgagggaatg
     1261 aaaggtcaat cttacgaagc gcaggtgacg aaaggtaaaa ccacgacgat tctgcgcacg
     1321 ttcggctcga ccgaagacgt tctgctcgct ctggacgcga aagccgtcga aacgcaaacg
     1381 aaaatcaagc ttcgcattac cggcgatttg attgatttgg agcgcgagca caacccgcag
     1441 gacattatgc gcgcggcggt gcagacaaat gtgacgagaa tgattgatac gacggtcggg
     1501 cgcgttattt tcaacgaccg cctgcagcgt gacaacctgc cgttcgtcaa cggaacgctc
     1561 aagaaaaaag gcttgcagtc gctcgttact ttcgcgcatt tgcggctcgg acacgagcag
     1621 accgtcgcgc ttttggacga tttgaaaacg ctcggatttc tctacgcgac gaaatcggga
     1681 atttccatcg gcatcgacga tatggtcacg ccgccgaaca aaaaggatat tatcaaagcg
     1741 gcgaacgagg acgtcaacaa actgcaattg caatacgaag acgcgacgat gaccaatatg
     1801 gagcgcgtca ataaagtgac ggctatctgg tcggaagtca cagaccacgt ggcgaaggaa
     1861 atgttcaagg cgatgaacga ccgcgaaaaa gccagaaacg agatgaatcc gattctcgtg
     1921 atggctgatt cgggagcgcg cggctcggaa gcgcagattc gtcagcttgc cggtatgcgc
     1981 gggctgatgg cgaaaccgaa cggcgagatt atcgaaacgc cgattgtcgc caacttccgc
     2041 gaggggctga acgttttgca atactttatc tcgacgcacg gcgcgcgcaa aggcttggct
     2101 gatacggctc tgaaaacggc tgattcgggc tacctgacgc gacgtctggt tgacgtggct
     2161 caggacgtga ttatctccga gccggactgc ggaactctga aaggcatccg cgccgaagcg
     2221 attgtccgca acggcgaaga agtcgaaagc ctgcgtgacc gcatcgtcgg tcgcacatct
     2281 ctggacgata tgattgaccc ggttgacgga aatataatcg ttcaggcaaa cgaagaaatc
     2341 aacgaagatt tggcgtcgaa agttcaaatg tcgggtttgc tgagcgtcaa gattcgctcg
     2401 gtgctgacct gcgaatcccg tcgcggcgtt tgtatgaaat gttacgggcg caatctggcg
     2461 accggacgca gcgtcgaaat cggcgaagcg gtcggcgtca tcgcggcgca aagtatcggc
     2521 gagccgggaa cgcagctgac gatgagaact ttccacgtcg gcggcacggc acggctcgaa
     2581 caggaaacca agcacgtcgc ggcgatggac ggaacggtta aatattccga agaactgaaa
     2641 gtcatcacca accgcgaagg cgagcgcatc tcgattcgcc gccagacgga gttgatgctg
     2701 gtcgacgacc gcgggcgcga agtggcgcgt tatcaaatcg tctacggcgc gcacgttcag
     2761 gtcgcggacg gtcagaaggt caaggaagac gaagtgctgg cgacgtggga cccgtttact
     2821 ttcgccattt tgacggaagt gccgggaact gtgaaattcc aggacctgaa agaaggcaaa
     2881 acggtcgaag aagacgtcga taaagtttcg ggacagaaac gcctcgtggt caaagactcg
     2941 gatgagaaac atcagccgcg tctggaaatc cgcagcgccg ccaacaaggt tctgaaaact
     3001 taccagatgc cgattcgcgc caaccttcac gtcgaagacg gcgcggaagt tcaggcgggc
     3061 gacattatcg ctaaaatccc gcgcgagacg acgaaaacaa aagacatcgt cggcggtctg
     3121 ccgcgcgtcg tcgaattgtt cgaagcgcgt cgtccgggcg agacagccgt gatgtcggaa
     3181 atcgacggaa cggtgaaatt cggcgccatc tccaaaggta agcgcaaaat cgttatcacc
     3241 ggcgacgaca acgccgagcg cgaatacgac attccgcgcg gaacgcacat caacgtgcag
     3301 gaaggcgacc gcgtcaaagc gggcgagccg ctgatggacg gacctttgaa cccgcacgat
     3361 attttgcgcg tgctcggaac cgaagcgctg caggaatatc tggtcaacga gattcaggaa
     3421 gtttaccgtc tgcagggcgt tttgattaac gataaacaca tcgaggttat cgttcgtcag
     3481 atgcttcgct gggtacgcat caaggatgtc ggcgacacgg atttcctttt ggaagaacag
     3541 gtcgaccgtt tccgttacga agacgaaaac cgtcgcgtga ttgacgagga cggacagcct
     3601 tcgtcggctg agccgatgct gctcggcatc acgaaagcgt cgctttcgac cgattcgttt
     3661 atctcggcgg cgtcattcca ggaaacgacg cgcgttttga ccgaagcggc gatttcagga
     3721 cgcatcgatt acctgcgcgg gctgaaggaa aacgtcatta tgggacgcct gattccggcg
     3781 ggaacgggta tgaagtcgta tcgcaacatc gtcatcgacc acgacccgac gatgaaccag
     3841 aaaaagaccg acgaatacga cgaatacgcc gatattcgcg gcggtctgga tttgcccgaa
     3901 tcgatggacg tgccgtttgt cgaagttgac ggcgacctcg acggattttc gctcgaagat
     3961 tcggacggcg acccgattct ggacgaggcg gaaacggaag aagtctttga agtcgaaggc
     4021 tcgctcggcg atttcgtcga tgacgaggac gacgattttt aagacgaaaa cacagaggca
     4081 taaacaataa ggtaaaaagg ctgagagaga aatctttcag ccttttttag taattggcgc
     4141 gcgattcggg cgtcctttta ctcagcagaa accggacgcc gatttataat attttcaaaa
     4201 tgatttaata aatgtatgct ctgtaaatgt gttcgcactg aaatcgacgg aagacggaaa
     4261 atgaaaaaaa tgttgtttcg aaatttatac ttattcctgt ttactattat ttatctggtc
     4321 tttatcaacg gttgtaatga aataaaaaaa gaaaatccgg taagcgcgcc gacgaaggct
     4381 gccgcgcctg acgttaaaaa tgaaccgaaa agcgcgctgc acaaacgaat tgaagaaata
     4441 gccggcaacg cgcagggaaa agtcggagtt gcggcgctcg ttctggaaac cggagaatcg
     4501 ttcggcttaa acgaaaacca gcatttcccg atgcagagcg tttacaagtt tcccatcgcg
     4561 atggctgtgc tcaaacgggt cgagcaggga aatctgaaat tggagcagaa gatacgcatc
     4621 gaaaaaagcg atttggttcc gaaacggctt cgaagcccga ttcgcgacag gtatccgcag
     4681 ggcgcggaac tcggtctggc tgaggtgttg aagtattcgg tgtccgacag cgacggaacg
     4741 gcttgcgacg tattgttaaa agcggtcggc ggagcggatg cggtgacaaa atatctgcgc
     4801 gaaatcggcg tcggcgatat ggtcgtcgcc accaccgaga aagagatggc gcaggacgaa
     4861 tcggttcagt atcaaaactg gtcttcgccg aaagccgcca acgatttatt gcgcttactg
     4921 cacgaaggac gcactctctc ggaaaacagc cgcgccctgc tgctgcaatg gatgacggaa
     4981 acgccgacgg gcgcaaagcg catcaaaggc ttattgcccg ccggaactgt cgtggcgcat
     5041 aaaaccggca cttccggcac ttttgacgga ttaacgcgcg cgaccaatga tgtcggactc
     5101 atcgctctgc cggacggcag acatctgtct atctcgattt tcgtctccga ttcgaaggcg
     5161 gatgaaaaga cccgcgaaga agttatagcg aaaataacgc gggcagtctg ggatgaattt
     5221 gttaaatagt ggtaagttgc aatttcaaag taacaactta ccacttaccg ttaaagactt
     5281 actaattcgt tattaccaaa tcagacacgc gtccgttctg accatcttgt cgcccgactt
     5341 gcagccgaag gcttgggcga attgcggcat attcgagagc ggaccgttga cgcgccagcg
     5401 cggcagcgag tgcgtgttgg tcttgacctg caaaatttcg gcttccggcg tgtacttgcc
     5461 cgcccagacc tgcgcccagc cgaggaaaaa tctctgttcg ggcgtaaatc cgtcaatctc
     5521 cgacggtcgc ggcttgcctt ccagagattt tttgtaagcg tcgtaggcga cggtcaagcc
     5581 ggcgaggtcg ccgatgtttt cgccgagcgt cagcttgccg ttgataaaca gaccgggctg
     5641 aacttcgtat tcgccgaact gtttgacgac gcaggcggcg cgctcctcaa atttggcgcg
     5701 gtcttctttc gtccaccaca ttttcagatt tccttcggcg tcgaagcggc ttcccgaatc
     5761 atcgaagccg tgcgagattt cgtgcccgat aacgccgccg atagcgccgt aatttatcgc
     5821 gtcgtctgcc gccgggttaa agaaaggcgg ctgcaaaatt ccggcgggaa acgtgatgtc
     5881 gttgttcacg cccgaatacg aagcgttaac ggtcggcggc gtgaaacccc agcgcgagcg
     5941 gtcgactggc tggttaatat ccagcagatt gcggcgcacc tgaaattgac cggagcgcaa
     6001 gacgtttgcc gaataagaat cgcgcttgac ggacaagccg ttgtatccgc gcagtttatc
     6061 gggcgagccg attttgcgct tgaaggtcgc gagcttcgcc tgcgcctgtt ttttcgtttc
     6121 ggcgctcatc cagtccagct tgttgacgcg gtctttcatc gccgccagca gattgtctat
     6181 catctcgttc atccgcgcgc gggcggcggg cgtaaaggct tttttgacgt attcctgacc
     6241 gagcgcttcg ccgagatgat tgtccacggc gttgacgcag gttttccagc gcggctgctg
     6301 ttctttcgtg ccgctcaaat atctggagta aaaattgaaa ttctcgtcgg caaaagcttt
     6361 agagagcgtc ggcgcggcgg cgtttatcgt catccagcgt aaataagtct tccagtcttc
     6421 cagcgaaact tccctgagca tcaggtttac ttccttgaaa aaaaccggct gaccgagatt
     6481 gatttccgtg accgccggga cgccgcgcgc ggtcata
//
LOCUS       DASWNE010000089         5314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_532999, whole genome shotgun sequence.
ACCESSION   DASWNE010000089 DASWNE010000000
VERSION     DASWNE010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5314
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532999"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1638)
                     /locus_tag="VGB68_01830"
     CDS             complement(<1..1638)
                     /locus_tag="VGB68_01830"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY0047995.1"
                     /translation="MAMTVSNYLITRLKQAGLEHMFAVPGDYASPFLQALDATKGIER
                     IPNINELGSGYAADGYARYKGIGAACVQYGVGTFSLLNCTAGSFVERVPVAVISASPS
                     TKDRAQEQSEDVLFHHSTGNLRADQIVFKNVTVASVIIDNPETAPAQIDRAMTSMLTH
                     RRPIYIEVLNDVWSMKCAAPKGKLAAVVTPSDADSLAAALDATFERIEAAKLPVLWAG
                     VEIQRFGLQATLQQIVDASGWYFTTTSLGKTVLDESRKQFIGTYAGPASPAKTWDAMC
                     AADCVLALGTIITDDYLNIMNSSFGKMIEVTDEETRVGYQYYHDVTLKDFLEGILTRL
                     KANKRYPRKYALPKTEPEEEYKAKPGDKLTYNVFYHELSNFLKKEKLLNDVALILGES
                     TSLYVFGNLFGLPPNSFVAQAAWGSLGHETGCALGVALGSGKRPLVVAGDGGFRMVCQ
                     ELSSLAYQKCNAVVFVMSNEVYAIEQAFVDIKAFTPKGEFAPFDVLPSWDYLALAQSF
                     GAKGFRAKTVEELRAVLSQLNDIKDAPALVEIMIPQKD"
     gene            complement(1643..3361)
                     /locus_tag="VGB68_01835"
     CDS             complement(1643..3361)
                     /locus_tag="VGB68_01835"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0047996.1"
                     /translation="MDLDIAVVGGGVAGAYCAWRLQQEKGAKAKIALFEYSDRIGGRL
                     YTVTMPGLPNVKAELGGMRYIPSQHVMVTNLVEHLGLPTKEFPMGAPAPVGSNCNIFY
                     LRGKHLRLHELNDPKKVPYDLAWSERGLGPTNLQVQVMNNIYPNFANLSLCDQMKVKV
                     FGKPIWQYGFWDLMYRVLSNEGYQFMKDAGGYDANVANANAMTQLPATEYNDETVFLT
                     LRDGYDQLPITLAERFKEKPGGIAKDDRVQMNHRLAEILIGTGEYRYTLIFERTITKD
                     GQTTIKHGSTIKVRAKKLILAMPRRSLELIKSKFFEDSWLKANIPSVLIQSAFKLLLA
                     YERPWWRALGLVAGRSVTDLPIRQVYYFGTEAEQPGGLPFMNSLLMASYNDISTVPFW
                     KGLEFGEPFKGYTPSCLEPGVTDIVPKSEFPATEEMVYVANRQVAQVHALPEIPQPYS
                     ARYHTWNEDPYGGGWHEWKANYRLDEIMCRMRKPVANEDIHIVGEAYSIGQGWVEGAL
                     DTAESALQDFFGLKPPKWLPKGTQLMPNPCPGCGDLDGCVEFSDPGGKILDLITPNCL
                     QSVEEG"
     gene            complement(3969..5138)
                     /locus_tag="VGB68_01840"
     CDS             complement(3969..5138)
                     /locus_tag="VGB68_01840"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HEY0047997.1"
                     /translation="MKAVCWYGKHDVRVENVPDPQILNSRDAIIKITTTAICGSDLHL
                     YNGYIPTMQEGDILGHEFMGEIVELGKDVTNLQKGDRVVIPFTIACGNCFFCERDLWS
                     CCDNSNPNAGIAETLYGFSGSGLFGYSHMLGGFAGGQAEYVRVPFADTGALKVPDSLT
                     DEQVLFLSDIFPTGYMAAENCDIKEGDTVAVWGAGPVGQFAVRSAFLLGAEQVVAIDH
                     YADRLQMAAEGGARTINFDDEDVFEKLKDLTGGRGPDSCIDAVGLEAHGVAPDALLDY
                     VKAKLFLATDRPHALRQAIHCCRKGGTVSVPGVYGGFLDKFNFGAAFGKGLTFKMGQT
                     HMHKYMPKLLSLVEEGKIDPTFVISHRLKLDDAPTAYSEFNKEKNQWRKVVLKPQ"
BASE COUNT         1199 a         1472 c         1360 g         1283 t
ORIGIN      
        1 atccttctgc ggaatcatta tctcgaccag cgcgggcgcg tctttaatgt catttaattg
       61 agacagcacg gcgcgcagct cttcgacggt tttcgcgcga aagccttttg ccccgaagct
      121 ttgagccagc gccagataat cccacgacgg aagcacgtcg aacggggcaa actcgccttt
      181 cggagtgaac gctttaatat ccacaaacgc ctgttcaatc gcataaactt cattgctcat
      241 cacgaaaacg acggcgttgc atttttgata tgcaaggctc gacaattcct ggcaaaccat
      301 gcggaagccg ccgtcgccag ccacgaccag cgggcgcttt ccgctgccca gcgccacgcc
      361 cagcgcgcag cccgtctcgt gtccgagcga gccccaagcc gcctgcgcga caaaactgtt
      421 cggcggcagc ccgaaaaggt ttccgaaaac ataaagcgaa gtgctttcgc cgagtattaa
      481 ggcgacgtcg ttcagcagct tttccttttt cagaaaatta ctcagctcgt gataaaaaac
      541 gttataagtc agcttgtcgc ccggtttcgc cttgtattct tcttccggtt cggttttcgg
      601 caaagcgtat ttgcgcggat agcgtttgtt tgccttgagc cgcgtcaaaa ttccttccag
      661 aaaatctttc agcgtgacgt cgtgataata ctggtagccg acgcgcgttt cctcgtcggt
      721 cacttcaatc atctttccga aactggaatt cataatgttc agataatcgt cggtgatgat
      781 tgtgccgagc gcgagaacgc agtccgccgc gcacatcgcg tcccacgttt tagccggcga
      841 agccggaccg gcgtaagtgc cgataaattg tttccggctt tcgtccagaa cggttttgcc
      901 gaggctggtc gtcgtaaaat accaaccgct cgcatcgaca atctgctgca aagtcgcctg
      961 cagtccgaaa cgctgaattt ccacgcccgc ccacaaaacc ggcagctttg ccgcttcgat
     1021 acgctcgaat gtcgcgtcga gcgcggcagc cagcgaatcc gcatccgacg gcgtcacgac
     1081 ggctgcaagc tttcctttcg gcgcggcgca tttcatcgac cagacgtcgt tcagcacctc
     1141 gatgtaaatc ggtctccggt gcgtcagcat cgacgtcatc gcgcggtcaa tctgcgccgg
     1201 agcggtttcc ggattgtcga taatcacgga cgcgaccgtc acgtttttaa aaacaatctg
     1261 gtcggcgcga agatttcccg tgctgtggtg aaacagaacg tcttcggatt gttcctgcgc
     1321 gcggtctttt gtgctcgggc tggcggaaat caccgccacc ggcacgcgct cgacgaaaga
     1381 accggcggtg cagttcagaa ggctgaaagt tccgacgccg tattggacgc aggcggctcc
     1441 gatgcctttg tagcgagcat aaccgtcggc ggcgtaaccg cttccgagct catttatatt
     1501 cggaatgcgc tcgatgcctt tggtcgcgtc gagcgcttgc agaaacggcg aagcgtaatc
     1561 gcctggaacg gcgaacatat gttccagtcc agcttgtttg agccgcgtaa tcaggtaatt
     1621 tgaaacggtc attgccatct ttttaaccct cctccacaga ttgcagacaa ttcggcgtaa
     1681 tcaaatccag aatcttgccg cccgggtcgc tgaattccac gcacccgtcc aaatcgccgc
     1741 agcccggaca cgggttaggc attaattgcg tgcccttcgg cagccatttc ggcggcttca
     1801 gaccgaagaa atcctgaagc gcggattccg ccgtgtcgag cgcgccttcg acccatccct
     1861 gcccgatgga ataagcctcg ccgacgatgt gaatgtcctc gttagcgacc ggcttgcgca
     1921 tccggcacat aatttcgtcg agccgataat tggctttcca ctcgtgccag ccgccgccgt
     1981 aagggtcttc gttccaggtg tgataacgcg ccgaataagg ctgcggaatt tcgggcagcg
     2041 cgtgaacctg cgcgacctgc ctgttagcga cataaaccat ttcttcggtc gccggaaact
     2101 cgcttttcgg aacaatatcg gtcacgcccg gctcaagaca cgacggcgta taacctttaa
     2161 acggctcgcc gaattccaag cctttccaga acggcacggt gctgatgtcg ttgtagctcg
     2221 ccatcagaag ggaattcata aacggcaagc cgcccggctg ctcggcttcc gtgccgaaat
     2281 aataaacttg ccggattggc aaatccgtga cggaacgacc ggcgaccagc ccgagcgcgc
     2341 gccaccacgg tctttcgtaa gccagaagca atttgaaagc gctctggatg agaaccgaag
     2401 gaatgttggc tttcagccac gaatcttcaa aaaatttgct cttgattaat tccagcgagc
     2461 ggcgcggcat cgcgagaatc aattttttcg cgcgtacttt aatcgtcgag ccgtgcttga
     2521 tggttgtttg tccgtctttc gtaatcgtcc gctcgaaaat cagcgtgtag cgatattcgc
     2581 ccgtcccgat aagaatttcc gccagacgat gattcatctg cacgcgatcg tctttggcga
     2641 tgccgcccgg cttttcctta aatcgctccg ccagcgtaat cggaagctgg tcgtagccgt
     2701 cgcgcagggt cagaaaaacg gtctcgtcgt tatattccgt cgccggaagc tgcgtcatag
     2761 cgttggcatt agcgacgttt gcgtcgtagc cgcccgcgtc cttcataaac tggtagccct
     2821 cgttgctcag cacgcggtac atcaaatccc agaagccgta ttgccaaatc ggtttgccga
     2881 aaaccttgac tttcatctgg tcgcacaggc tcagattggc gaaattcggg taaatattat
     2941 tcatcacctg aacctgcaaa ttagtcggac ccaatccgcg ctcagaccac gccaggtcgt
     3001 aaggcacttt tttcgggtcg ttcagctcgt gcaggcgcag gtgctttccg cgcagataga
     3061 aaatattgca gtttgagccg accggcgcgg gcgcgcccat cggaaattct ttggtcggca
     3121 agccgagatg ctcgaccaga ttcgtgacca tcacgtgctg cgacggaata taacgcattc
     3181 cgcccagctc cgctttcacg ttcggcaagc ccggcatcgt caccgtgtaa agccgcccgc
     3241 cgatgcggtc gctgtattca aagagggcga ttttcgcctt cgcgcctttt tcctgttgaa
     3301 gccgccacgc gcaataagca ccggcgacgc ctccgccgac gacggcaata tccaagtcca
     3361 tattcagctc ctttagtaag taaactcaag ttgccggcga taaaacttta gaaagagttt
     3421 gtccgcgaaa gacgcgaaac cgcgccaaaa tttgattggt tttttttcgt ctgttccgtg
     3481 tgtttcgtgg gcatattttc ttaaattcga ttaaccggca gcctcggtcg agtaatgcga
     3541 ttcgttgttt tgaaatcgtt tcggtttatt gctttccagt gctttttaag ctccttgata
     3601 aacgggttta tgtatagcat agcttcaatg aaaaccgctg ctgcggcagg aaacgcggcg
     3661 agaaaaagca cctgctttca ggaaagcccg caggatgaaa aaaatagtag cttcgaagaa
     3721 gacgccttgt caataatttt atttaaaaac ttatcgaaaa agtgattcgc cgctcgcaat
     3781 attttgtaat tcagggattc aggattcagg attcaggatt caggattcaa gatttgggat
     3841 ttaagattca ggattcagga ttcaggattc aggattcagg attcaggatt tgggatttaa
     3901 gattcaggat tcaggattgg aaatcgaatt aatcctgaat cctgaatcct gaatcctgaa
     3961 tttttaactt attgcggttt cagcacgact ttacgccatt gatttttttc cttgttaaat
     4021 tcgctgtaag ctgtcggcgc gtcgtcgagc ttcaggcgat gcgagatgac gaaggtcggg
     4081 tcgattttgc cttcttcgac cagcgacaaa agcttcggca tatatttgtg catatgcgtc
     4141 tgtcccattt tgaacgtcaa gcctttgccg aacgccgcgc cgaaattgaa tttatccaga
     4201 aatccgccgt agacgccggg cacggaaacc gttccgcctt tccggcagca gtgaatcgcc
     4261 tgccgcagcg cgtgcgggcg gtcggtggcg agaaataatt tggctttcac gtaatcgagc
     4321 agagcgtcgg gcgcgacgcc gtgcgcttcc agtccgacgg cgtcgatgca cgaatcggga
     4381 ccgcgcccgc cggtcaaatc ctttaatttc tcgaaaacgt cttcgtcgtc gaaattaatc
     4441 gtccgcgcgc cgccttccgc cgccatctgc aggcggtctg cgtaatggtc aatcgcgacg
     4501 acctgttccg cgccgagcag aaacgcgctt ctgacggcga attgcccgac cggacccgcg
     4561 ccccagacgg cgaccgtgtc gccctcttta atatcgcagt tttccgccgc catatagccg
     4621 gtcgggaaaa tgtccgaaag gaaaagaacc tgttcgtccg tcagcgaatc gggaactttc
     4681 agcgcgccgg tgtcggcaaa cgggacgcgc acgtattcgg cttgcccgcc cgcgaagccg
     4741 ccgagcatat gcgagtaacc gaacagtccg gaaccggaaa aaccgtaaag agtttcggcg
     4801 attccggcgt tcgggttcga attgtcgcag cacgaccaca aatcgcgctc gcagaaaaaa
     4861 caattgccgc aggcaatcgt aaacggaatc acgacgcggt cgcctttttg cagattcgtc
     4921 acgtctttgc cgagctcgac gatttcgccc ataaattcgt gtccgagaat atcgccttcc
     4981 tgcatcgtcg ggatataacc gttatagaga tgcaaatccg agccgcaaat ggcggtcgtc
     5041 gtaattttga taatcgcgtc gcgcgaattc aaaatctgcg ggtcgggcac attctcgacg
     5101 cgcacatcgt gttttccata ccaacaaact gctttcattt ttatttctcc cttgttcgta
     5161 aaattttagc cacagagagc acagggattt ttaagaggga agttaacagt gatgaagaga
     5221 tgaagaagat aagaacaaaa agcattaatc ttttatctcc gttctccgct tcatccctgt
     5281 taattcgctt gtgtttatct gtgtaaatct atgg
//
LOCUS       DASWNE010000090         3033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_250464, whole genome shotgun sequence.
ACCESSION   DASWNE010000090 DASWNE010000000
VERSION     DASWNE010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250464"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VGB68_01845"
     CDS             <1..507
                     /locus_tag="VGB68_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047998.1"
                     /translation="YFPETWKEFTYETDGVRFRFPSTPSVTVSTETMSDQKVTTRKYT
                     RHTFMAMELWVVEYSADYNLEEFNPIKETLQNLRGVALDSIKDSNPKVIKESDITVDG
                     HAGKFMHVETNKGEILRFKFFLVKNRMYYAFAAVKKGEKHGSNYENDFEKAAMGFLDS
                     IKLTPAKK"
     gene            complement(575..736)
                     /locus_tag="VGB68_01850"
     CDS             complement(575..736)
                     /locus_tag="VGB68_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0047999.1"
                     /translation="MIAPTPKDTKTNAARQTERAKDTSLKSDRQNGGGNYFSIHNQFL
                     IYSGMPQPI"
     gene            complement(862..1998)
                     /locus_tag="VGB68_01855"
     CDS             complement(862..1998)
                     /locus_tag="VGB68_01855"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEY0048000.1"
                     /translation="MPPARISREKVLAEAHVEKEPIMKYSRLILLIIFTLIFSVAAVG
                     QRKFSGTVVDVVDGRTVVLEMPAGGKLTFELQYIEVPESEQQLHQTVREHLQQMVYGK
                     QVEFLPRGFTETKTLGQLFVGGVDVSQQMLRDGAAWHISPEISGQDEAGRKNYIETEK
                     QAKEEKRGVWGIEGLKPAWEFREEKQKTLNAKISKEISEENKETKQPRKTQSNIGLNQ
                     FDLTQSKGEKELSVYKIPNNEKDAIKKSFEAVEMMRLFLKDLATVGIRLSEYTGRLAE
                     LRFEVEGASQGLPIGKLKILMNENVDILDDLGVVFKEMRENDFLYYRNFQSLSKKYSI
                     EPVRNNAGDFVLWRKDIISAMADKAFNNFSELRRTLKEQEKYTK"
     gene            complement(2035..>3033)
                     /locus_tag="VGB68_01860"
     CDS             complement(2035..>3033)
                     /locus_tag="VGB68_01860"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0048001.1"
                     /translation="RAQLERRNTEMPEIVAESPQMKEILRLVQRVARSETSVLITGES
                     GTGKELIAQALHRLSPRSALTFVDLNCAALQDNLLESELFGHEAGAFSGARARKMGLF
                     EIADGGTLFLDEVMEMPMALQSKLLRALETKSFFRVGGVRKVEVNVRLLAATNRRPDQ
                     ALSEGVFRSDLLYRINSFEICIPPLRERREDIEPLARHLLHKIAGAHAPELAPQAVEA
                     LCSYHWLGNVRQLRNCLERAVLLADNGRITTKELPPEVVYKTPSTVSVSYNVPESTAT
                     NSFQNASPTALRDVEKQQIISALEKTGWHRGKTAELLGISPSTLYRRLREYDLDVR"
BASE COUNT          754 a          748 c          691 g          840 t
ORIGIN      
        1 tattttcccg aaacctggaa ggaatttact tacgaaacgg acggcgtaag atttcgtttt
       61 ccgtcaacac cgtctgtaac ggtttccacc gaaactatgt ccgaccagaa agtaaccaca
      121 cgaaaatata cgcgtcacac atttatggcg atggaattgt gggtggtcga atactctgcc
      181 gattacaatc tcgaagaatt caatccgatt aaagaaaccc tgcaaaattt gcgcggcgtc
      241 gcgctcgaca gcattaaaga ctcgaatccc aaagttatta aggaatccga cattacggtt
      301 gacggtcacg ccggaaagtt tatgcacgtc gaaaccaata aaggcgaaat cctgcgcttc
      361 aaattctttc tcgtcaaaaa ccgtatgtat tacgctttcg ccgccgtcaa aaaaggcgaa
      421 aaacacggct ctaattacga gaacgatttt gaaaaagcgg cgatgggctt tctggattcg
      481 ataaaactga ctccggctaa gaaataattg ccgggaattg ttaagcccga cttttcggcg
      541 gcgaaaaata ctttaacttt acgagacgaa tttattaaat cggctgaggc atacctgaat
      601 aaatcaaaaa ctgattatga atagaaaaat aattgccgcc gccgttttgc cggtcggatt
      661 ttaatgacgt atctttcgcc cgttcggttt gtcgcgccgc attagtcttt gtatctttcg
      721 gggtgggcgc aatcatctat tttctcgaat attttctcga aaaaaataaa acggcagccg
      781 aaaacagccg ccgtcaaatt tgcctttgta atcgctgaaa ttaatttaaa gactttgatg
      841 agttatcatt taattgataa ctcatttagt atatttttcc tgttctttta acgtgcgccg
      901 gagttctgaa aagttattaa atgctttatc agccattgcg gatataatgt cttttcgcca
      961 taaaacgaaa tctccggcat tatttcttac tggctcgatt gagtattttt tagaaagtga
     1021 ttgaaaatta cgataataca gaaaatcatt ctcacgcatt tctttaaaaa caacacctaa
     1081 atcatctagt atatctacat tttcattcat caaaatcttc aatttaccga ttggaagtcc
     1141 ctgagatgct ccttctactt caaaccttaa ttcagccaat cttcctgtat attcactaag
     1201 tctaattcca actgtcgcta aatccttcag aaaaagtctc atcatctcaa ccgcctcaaa
     1261 tgactttttg atagcgtctt tttcattatt tggaatttta tagacagata attctttctc
     1321 tcctttgctt tgagttaaat caaattgatt tagccctata ttgctttgag tttttctcgg
     1381 ttgttttgtt tccttatttt cttcgctaat ttctttgcta atttttgcgt taagagtttt
     1441 ttgtttttcc tctctaaatt cccaagcggg ctttaaacct tcaattcccc aaaccccgcg
     1501 tttttcctct ttagcttgct tttccgtttc aatatagttt tttcttccag cttcgtcctg
     1561 accgcttatt tccggcgaga tgtgccacgc cgcgccgtcg cggagcatct gctggctgac
     1621 atcgacgccg cccacaaaaa gctgtcccag ggttttggtt tcggtaaagc cgcgcggcag
     1681 aaattccacc tgcttgccgt aaaccatctg ctgcaaatgt tctctgaccg tctgatgaag
     1741 ctgctgttcg gattcgggaa cctcgatgta ttgcagttcg aaagttaact taccgcccgc
     1801 cggcatttcc agcacaaccg tcctgccgtc gacaacgtca actaccgttc cgctgaattt
     1861 gcgctgaccg acggcggcga cgctaaaaat cagcgtgaaa ataattaaga gaattaatct
     1921 ggaatatttc ataatcggtt ccttttcaac gtgagcttca gctaacacct tttcacggga
     1981 gatgcgcgca ggcggcacaa agcttttaag aaaatcagcg gttaatcggt ttctttatct
     2041 gacatccaaa tcgtattcgc gcaggcggcg ataaagcgtc gaaggcgaaa tgccgagcag
     2101 ctccgccgtt ttgccgcgat gccaaccggt tttttccagc gcgctgatga tttgctgttt
     2161 ttccacgtcg cgcagggcag tcgggctggc gttctggaaa gaatttgtcg ccgtcgattc
     2221 cggcacgttg tagctgacgg agacggtcga aggcgtttta tagacgactt cgggcggcag
     2281 ctctttggtc gtgattctgc cgttgtcggc aagcaaaacg gcgcgttcca gacaatttcg
     2341 cagttgacgg acattgccga gccagtgata cgagcaaagc gcttcgactg cctgcggcgc
     2401 aagctccggc gcgtgcgcgc ccgcgatttt gtgcaaaagg tgacgcgcca gcggctcgat
     2461 gtcctcgcgg cgttcacgaa gcggcggaat gcaaatctcg aagctgttga tgcggtaaag
     2521 caaatccgag cggaaaacgc cttccgacag agcctggtcg gggcggcgat tcgtcgcggc
     2581 gagcagtcgg acattgacct cgactttgcg gacgccgccg acgcggaaaa acgatttcgt
     2641 ctccagcgcg cgaagcagtt tcgattgcag agccatcggc atttccatca cctcgtcgag
     2701 aaacagagtt ccgccgtcgg caatttcgaa aagtcccatt ttacgggcgc gcgcgccgga
     2761 aaacgcgccg gcttcgtgcc cgaaaagctc ggattcgagc agattatcct gtagagcggc
     2821 gcagttaaga tcgacgaaag tcaaagccga gcgcgggctg aggcgatgca gagcctgcgc
     2881 gataagctct tttcccgtcc cggattcgcc cgtaatcaaa acggaagttt cagagcgcgc
     2941 gacgcgctgg acgagccgca gaatttcttt catctgcggc gattcggcga cgatttcggg
     3001 catttccgtg ttgcggcgtt cgagctgcgc gcg
//
LOCUS       DASWNE010000091         3979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_285734, whole genome shotgun sequence.
ACCESSION   DASWNE010000091 DASWNE010000000
VERSION     DASWNE010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285734"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VGB68_01865"
     CDS             <1..205
                     /locus_tag="VGB68_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048002.1"
                     /translation="GDTLIDGSRFSLLGNILDQKQQSFTRYGTRPAVLSGGNKKSFAR
                     RGKFRFYWKNLFLFERGAVGFHN"
     gene            complement(202..573)
                     /locus_tag="VGB68_01870"
     CDS             complement(202..573)
                     /locus_tag="VGB68_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048003.1"
                     /translation="MPSTAIACGILLILIGVIGYVYGMTTNENASPTALIPAAFGLLL
                     AILGAAARAKENLRMHIMHAAVLVGLIGFMVPAARVLSNLGGITLSAAVISQISMALV
                     CLIFVILCVKSFIYARRSRVA"
     gene            complement(853..2838)
                     /locus_tag="VGB68_01875"
     CDS             complement(853..2838)
                     /locus_tag="VGB68_01875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEY0048004.1"
                     /translation="MKFNFRSNLLLLFILIACAFGSINAQTPSPSPTPAPNPQRDYIA
                     ANYTKREILIPMRDGVKLFTSVYEPKDKSVKYPILMVRTPYTVAPYGETIKNVLGPNQ
                     LYAKEGYIFVYQDVRGRWMSEGQFEDIRPDIENRTPKDIDESTDTYDTIDWLVKNVGN
                     NNGRVGIYGISYPGFYASAGSIDSHPALKACSPQAPVSDWFQGDDMHHNGALFLAQNF
                     AFFSSFGQPRPAPTSTFDYLKPWKGRQTPDAYNFFLETGGLKEVADAYEKGLGIRIRF
                     WDEMMAHPNYDDYWKERNILPKLKNIKCSTMTVGGWYDNEDLFGALRTYQAIEKQNPG
                     IFNVLVVGPWDHGGWAGRDGDWLGTAYFGQKTGQYYRENMELQFFNHFLKDKGDISRI
                     KEVNLFDTGSHEWRAFDTYNPSEATETALYLQAGGGLSFEKPANGANGFDEYVSDPWN
                     PVPYTQKITLNYPRDFMTEDQRFAATRPDVLVYQTEPLAEDITVAGDIKPQLFVSSSG
                     TDSDFVVKLIDVFPDDYRYPETGNKLANGQPERIKPPEGFASTVFQTGGYQMLLRGEP
                     MPARFRNSFEKPEPLKPNEAARLAFVMPGVAHTFKKGHRIMVQIQSTWFPLVARNPQK
                     FMANYKMGTNADFQKATQRVYRGGGGASSIVLPIVKR"
     gene            3275..3946
                     /gene="nfi"
                     /locus_tag="VGB68_01880"
     CDS             3275..3946
                     /gene="nfi"
                     /locus_tag="VGB68_01880"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: protein motif:HMM:NF008629.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HEY0048005.1"
                     /translation="MQIKPLHDWNLSPREAIELQKQLAYEVIREDEFAAPIKTVAGID
                     LGYDAKNDTSRAVVVVLKFPELELSEKSEAILPIQFPYVPGLLSFRETPVAIKALEKL
                     QNAPDLILCDGQGLAHPRRFGIACHIGLLADVPTIGVAKSLLIGKFENLAETRGSLAP
                     LIHKNEQIGVALRTKDKVQPLYVSVGHRISLETAVRYVLQCAPKYRLPETTRLADKLA
                     SYRKR"
BASE COUNT          966 a         1046 c         1011 g          956 t
ORIGIN      
        1 tggagacaca ctcattgacg gctcgcgctt cagcctttta ggaaatatcc tcgaccaaaa
       61 acagcaaagt tttacccgtt acggaacgcg cccggcggtt ctttccggcg gaaataaaaa
      121 gtccttcgcc cgtcgcggaa agtttcggtt ttattggaaa aatctatttt tattcgagcg
      181 cggcgcagtc ggttttcata attaagcgac gcggctgcgg cgggcgtaga tgaaagattt
      241 gacgcacaga attacgaaaa tcagacaaac cagagccatc gaaatctgcg agatgactgc
      301 cgccgaaagc gttatgccgc cgagatttga aagcacgcgc gccgccggaa ccataaatcc
      361 gataagcccg accagaaccg ccgcgtgcat tatgtgcatc cgcagatttt ctttggcgcg
      421 ggcagcggct cctaaaatgg ctaaaagcaa accgaaagcg gctggaatca gcgccgtcgg
      481 gctggcgttt tcgttcgtcg tcattccgta aacgtagccg atgactccaa tcaaaatcag
      541 caaaattccg caggcaatcg ccgttgatgg cataattttt ctccttgaaa aaatgttctc
      601 agctaaaagc taccaaatct ttgctgaaaa gacgaaacgc ggaaaaatta aaaaaccgtg
      661 caaagcttaa aacgggtttt aatgttttcg ggcgaattat ttttcgtcaa tcgcccggag
      721 caggtcgcgg gctaaatttc cgttaagcgt cctgagcttt tcgggactga cgggataatt
      781 gcttcttttc atcctgctcg ctttagtccg tcccgtttga attttctttg tgtttgacgt
      841 gccgtttgaa aattatcttt tcacaatcgg cagaacgatg ctcgaagcgc caccgccgcc
      901 gcgataaacg cgctgggtcg ctttctgaaa atcggcgttc gtgcccatct tgtaattcgc
      961 cataaatttc tgcggattgc gcgccacgag cggaaaccag gtgctttgaa tctgcaccat
     1021 tatccggtga ccttttttga aagtgtgcgc cacgcccggc atcacgaatg ccagccgcgc
     1081 cgcttcattc ggctttaaag gttcgggctt ttcgaagctg ttgcggaaac gcgccggcat
     1141 cggctcgccg cgcagaagca tttgatagcc gccggtctgg aaaacggtcg aggcgaagcc
     1201 ttccggcggt tttattcttt ccggctgccc gtttgccagc ttgtttccgg tttcgggata
     1261 acggtaatcg tccggaaaaa cgtcgattaa tttcacgacg aaatccgaat ccgtgccgct
     1321 cgaagagacg aacaactgcg gcttgatgtc gcccgccacc gtgatgtctt ccgccaaagg
     1381 ctcggtttga taaaccagca cgtcgggtcg cgtcgcggcg aaacgctgat cttcggtcat
     1441 aaaatcgcgc gggtaattca aagtgatttt ctgcgtgtac ggaaccggat tccacgggtc
     1501 ggagacgtat tcgtcaaaac cattcgcgcc gtttgccggt ttttcaaacg aaagcccgcc
     1561 gcctgcctgc aaataaagcg cggtttcggt cgcctcgctc gggttataag tatcaaacgc
     1621 gcgccattcg tgcgagccgg tatcaaacag gttgacttcc ttgattcgag aaatgtcgcc
     1681 cttgtccttc aaaaaatgat tgaaaaactg aagctccata ttctcgcggt aatactgtcc
     1741 ggttttctgt ccgaaataag ccgtgccgag ccagtcgccg tcgcgtccag cccagccgcc
     1801 gtggtcccag ggaccgacga ccagcacgtt gaaaattccc ggattttgtt tttcgatggc
     1861 ttgatacgtg cgcagcgcgc cgaataaatc ctcgttgtcg taccagccgc cgacggtcat
     1921 cgtcgagcat ttgatatttt tcagcttcgg cagaatattg cgttctttcc agtagtcgtc
     1981 gtaattcggg tgcgccatca tctcgtccca gaacctgatg cggatgccca gacctttctc
     2041 gtaagcgtcg gcgacttcct tcaagccgcc cgtttcgagg aaaaagttgt aggcgtcggg
     2101 cgtctgcctg cctttccacg gtttcagata atcgaaagtc gaagtcggcg ccgggcgcgg
     2161 ctgaccgaaa ctgctgaaaa aggcgaagtt ctgcgccaga aaaagcgcgc cgttgtgatg
     2221 catatcgtcg ccctgaaacc aatcggagac gggcgcttgc ggcgagcagg ctttcagcgc
     2281 cgggtgcgaa tcgatcgagc cggcggaagc gtaaaaaccc ggatacgaga tgccgtaaat
     2341 gccgacgcgc ccgttgttgt ttccgacgtt tttgaccagc cagtcgatcg tgtcgtaagt
     2401 gtcggtcgat tcgtcgatgt ctttcggcgt gcggttttcg atgtccggtc ggatatcttc
     2461 aaattgtcct tccgacatcc agcgtccgcg cacgtcctga taaacgaaaa tatagccttc
     2521 cttcgcatac aactggttcg gaccgagcac gtttttaatc gtttcgccgt agggcgcgac
     2581 cgtgtagggc gtgcgaacca tcagaatcgg atacttgacg gatttatcct tcggctcgta
     2641 aacggacgtg aaaagcttca cgccgtcgcg catcggaatc aggatttcgc gcttcgtgta
     2701 attcgccgcg atgtaatcgc gctgcggatt cggcgcgggc gtcggcgacg gcgacggcgt
     2761 ctgcgcgttg attgaaccga acgcgcaggc aatcagaata aacagcagca ataaattaga
     2821 acggaaatta aatttcattt tgacctcgat tgattcttag aagaagccgg ctattgtcat
     2881 cagatagaaa cattgatttt tcctgagtag aatcttgtat gttgctataa acgcattttt
     2941 ctctgtttta gtttctatcc atttcagcgc cgttccgata tagacggtat ctgctctgca
     3001 atactcggcg ccgaccggaa cgcgtttcat ccataagtcg agccggcgaa aacgtttccc
     3061 gcaaaagcga aatccgaaac ggcgaaaaca aaaacgggga aagattgcag gcggaaaatc
     3121 ttcaaaatgc tctcccagac ataaaaatct gatgaattca gcctaatttt cgctcaatta
     3181 ataatcaaag acaagtgaat tttgaagccg cttatttttc agcccgcatc ttctaaaaaa
     3241 tccgcgcttt gctgtgttaa tctgtaatta tattatgcaa atcaaaccgc tccacgattg
     3301 gaacctgtcg ccgcgcgagg cgattgagct gcaaaaacag cttgcctatg aagtaatcag
     3361 ggaagacgaa ttcgccgcgc caatcaaaac ggtcgccgga atagatttgg gctacgacgc
     3421 caaaaacgat acgagccgcg ccgtcgtcgt ggtcttgaaa tttcccgaac tcgaactcag
     3481 cgaaaagagc gaagccatcc tgccgattca gtttccgtat gtgcccggtc tgctttcctt
     3541 tcgcgagacg cccgtagcga taaaagctct ggaaaaactg caaaacgcgc cggatttaat
     3601 tttatgcgac gggcagggac tcgcgcaccc gcgccgcttc ggcatcgcct gtcatatcgg
     3661 attgctcgcg gacgtgccga cgatcggcgt ggcgaaatct ctgctaatcg gaaaatttga
     3721 aaaccttgcc gaaacgcgcg gaagcctcgc gccgctgatt cataaaaacg agcaaatcgg
     3781 cgtcgccctg cggacgaaag ataaagtcca gcctttgtac gtttccgtcg gacatcggat
     3841 aagtctggaa accgccgtcc gatacgttct gcaatgcgcg ccgaaatatc gcctgccgga
     3901 aacgacgcga ctcgccgata aactggcttc gtatcgaaaa cgatagggca aaattaagat
     3961 aagcaattta taaacatca
//
LOCUS       DASWNE010000092         6060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_74238, whole genome shotgun sequence.
ACCESSION   DASWNE010000092 DASWNE010000000
VERSION     DASWNE010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6060
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74238"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..801)
                     /locus_tag="VGB68_01885"
     CDS             complement(178..801)
                     /locus_tag="VGB68_01885"
                     /inference="COORDINATES: protein motif:HMM:NF024176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY0048006.1"
                     /translation="MLDKSLWNEKTSHGEILKKVWKHLDLGVLEREHPFHTPVFGTSA
                     GGAVQLRVVVLRRFWRRNPRGLVFHTHAGSPKIAEIENNPNVSWLFYHPQERFQVCVK
                     GTATIRRDGEQLHEEQWLATEFFSRRCYVGEAPSQFSEKPTSGLPEDLTRRAPTEEES
                     EAGRENFAVIFSTIDRIDCLELNVKGHRRSLFVWTEAGELETKWLTP"
     gene            complement(1002..2009)
                     /locus_tag="VGB68_01890"
     CDS             complement(1002..2009)
                     /locus_tag="VGB68_01890"
                     /EC_number="1.1.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005962 - mitochondrial isocitrate
                     dehydrogenase complex (NAD+) [Evidence IEA];
                     GO_component: GO:0045243 - cytosolic isocitrate
                     dehydrogenase complex (NAD+) [Evidence IEA];
                     GO_function: GO:0004449 - isocitrate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NAD(+))"
                     /protein_id="HEY0048007.1"
                     /translation="MTKHTITLIPGDGIGPEIIAATVRIIEASGVDIEWETHILGAQA
                     LEKYGVTLPEPALESIKRNKVALKAPLTTPIGKGFTSVNVGLRKLLDLYANVRPIKAL
                     PNVESRYPELDLVVVRENTEDLYAGLEHVVIPGVVESIKIITEKASTRIAKYAFEFAR
                     DNNRKKVTAVHKANIMKLSDGLFLECFYNAAKDFPEIEADDKIIDNCCMQLVMRPEQF
                     DVLVMENLYGDIVSDLCAGLIGGLGLAPGANIGEIGAVFEAVHGSAPDIAGQGIANPT
                     ALLMSAIMMLRHLGEIDAASRVEKAMFEVFVEGKVRTKDLGGAAKTDDFANAIIDKMK
                     Q"
     gene            2711..3394
                     /locus_tag="VGB68_01895"
     CDS             2711..3394
                     /locus_tag="VGB68_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048008.1"
                     /translation="MFDEIDKHLESWARDVFEGGDLEISFAAPLAETEKPSVYLYLLD
                     VLPTPPGRGVRLPPLRITLRYLVVPQAKTPGESHRLLGKMLVSAMENAEFEVEKEPLS
                     LDLWRAFGIAPRPAFILRAAFKHDRKEQIAPPVREPVRLRQALLESLQGQISLNRIPL
                     VSAKVEIPLLKVFTQTDADGKFRFASLPSEPQDKSLLIRAKGREFSVSTGDAIRRGNL
                     FLIDLKLED"
     gene            3396..4616
                     /locus_tag="VGB68_01900"
     CDS             3396..4616
                     /locus_tag="VGB68_01900"
                     /inference="COORDINATES: protein motif:HMM:NF016845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HEY0048009.1"
                     /translation="MPTYQTPGIYIEEISTGTRPIEAVGTSTAGFVGTSPNADARVNE
                     AFAVNSWSQFIKEYVAEDSKSTPLSQAVFGFFQNGGSRCYVVNIGANGSLLGDGKRRQ
                     GLGLLEEIDEVAIVAAPGFTDTASYDALLEHCERMQDRFAILDAPATVSSIDALKKVG
                     AAGKPKKPEGETGAGGDTGGDTGGARPRTSDRGYGAFYFPWLIVNDPFSPGEPVIVPP
                     SGHLAGIYARTDATRGVHKAPANESVRGALDLAYRITQSEQGELNTLGVNCLRFFPTQ
                     GIRVWGARTLADSASEWRYVPVRRLFNMIEESIARGTRWVVFEPNDVTLWKSIARDIR
                     AFLTLQWRQGALMGATAEEAFFVQCDAETNPPEEIDAGRVIAVIGIAPVKPAEFVIFR
                     IGQSAAGTEIEGGI"
     gene            4619..5092
                     /locus_tag="VGB68_01905"
     CDS             4619..5092
                     /locus_tag="VGB68_01905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEY0048010.1"
                     /translation="MPPKADEAKPQAAPANAYVDPFRAYNFKLEIQGITEAHFTEVSN
                     MEVKVTPIAYREAGNSQVVHYVPGQTEYGAITLRYGLTRSRELWDWFMTGVKGKVQRK
                     NCSIIVLDADGATEVLRWNLINAWAAQWRGAMLNALDQEVAIESLTLVCETFERA"
     gene            5167..>6060
                     /locus_tag="VGB68_01910"
     CDS             5167..>6060
                     /locus_tag="VGB68_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048011.1"
                     /translation="MRLVDYKIKFFDFVAGNLRRLAELLTVEAAPEQEDSAPEKTLSP
                     MEDWLEKTRNLPAENWVDFSGANQPDAFRENESEPEAQPPNSSFAAETGAADFVPEKP
                     KKQTPVETFFKKKHEAAENRQTFGNLEASNKAASKTEVSRAEKPKKTKPESRFFSIKF
                     SDQKRKQENAEPLEPLAESFAQSTAKDQDRLEKAKRNFRLFPAEAASESSAETAAPLV
                     INFIENQPPKAEAPAFERKKSAAVKFEKIEPAKPKNTIEKTESIVKKSSARREISNQF
                     PPAAFSRKTGGESAADINFEFP"
BASE COUNT         1480 a         1638 c         1588 g         1354 t
ORIGIN      
        1 tcaattcggc tttagcgttg cacgtagaag attcgggatt caggatttaa gatttaagat
       61 tcggaattca ggatttagga ttggtcgttt gctaacggcg catcttaaat cctgaatttt
      121 aaatttaaaa ttctgaatcc cgaatcctga atcctgaatc ctgaatcccg aattgattta
      181 aggcgtcagc catttggttt cgagctcgcc cgcctccgtc cagacaaaca gcgagcggcg
      241 atgacctttg acgtttaatt ccagacaatc gatacggtca atcgtcgaaa aaatgacggc
      301 gaagttttcc cgcccggctt cggattcttc ttcggtcggc gcgcgcctcg ttaaatcttc
      361 gggcaagccg gaagtcggtt tttcgctgaa ttgcgaaggc gcttcgccga cgtagcagcg
      421 gcgcgagaaa aactccgtcg ccagccattg ttcctcgtgc agctgttcgc cgtcgcggcg
      481 aatcgtcgcg gtgcctttga cgcagacctg aaatctttcc tgcgggtgat agaaaagcca
      541 cgaaacgttc ggattgtttt cgatttcggc gattttcggg cttccggcgt gcgtgtgaaa
      601 gacgagcccg cgcggatttc tgcgccagaa ccgacgcaga acgacgacgc gcagctgcac
      661 cgcgccgccc gccgacgtgc cgaaaaccgg cgtgtgaaac ggatgctcgc gctccagcac
      721 tcctaaatcg agatgtttcc agactttttt gagaatctcg ccgtgactgg ttttctcgtt
      781 ccaaagactc ttatccaaca taaggaaaat tagccgcaga tttccgcagg caggaaaaag
      841 aaattaaccg acgataaagc agatgaagaa gtaagaaaag taaaaagcaa aaggtaaaag
      901 gcaaaattaa gagccgattt tttacttttt acttttgcct ttaaaccgct cgttcatcga
      961 cttcatcttc ggttaattca cccgcgttcg gctgcggtct cttactgttt cattttatca
     1021 ataatcgcgt tggcaaagtc atcggttttc gccgcgccgc ccaaatcttt cgtgcgcact
     1081 ttgccctcga caaagacttc gaacatcgct ttttcgacgc ggctggcggc gtcgatttcg
     1141 cccaaatgcc tgagcatcat aatcgccgac atcaaaagcg ccgtcggatt ggcgatgccc
     1201 tgcccggcga tgtccggcgc cgagccgtgg acggcttcaa acaccgcgcc gatttcgccg
     1261 atgttcgcgc ccggcgccag ccccaagccg ccgattaagc cggcgcacaa atcggaaacg
     1321 atgtcgccgt aaagattttc catcaccaga acgtcgaatt gttcggggcg cataacgagc
     1381 tgcatacagc agttgtcgat aattttatcg tccgcctcaa tctccggaaa atctttcgcc
     1441 gcgttgtaga agcattccag aaaaagcccg tccgacaatt tcataatgtt cgccttgtga
     1501 accgccgtga cttttttgcg gttgttgtcg cgcgcaaatt cgaaagcgta ttttgcaatt
     1561 ctggtcgaag ccttttcggt gatgattttg atgctttcga ccacgccggg aatgacgacg
     1621 tgttcgagcc cggcatacaa atcttccgtg ttttcgcgca cgacgaccaa atccagctcc
     1681 gggtagcgcg attcgacatt cggcagagct ttaatcgggc ggacattggc gtaaaggtcg
     1741 agcagctttc gcaagccgac gttgacgctc gtaaaacctt tgccgatggg cgtcgtcaaa
     1801 ggagctttca gcgcgacctt gtttctttta atcgactcca gcgccggttc gggcaaagtc
     1861 acgccgtatt tttcgagcgc ctgcgcgccc aggatatgcg tttcccattc gatgtcgacg
     1921 cccgatgctt cgataatgcg cacggtcgcg gcaataattt ccggtccgat gccgtcgccc
     1981 ggaatcagcg taatagtgtg tttcgtcatt ctaattactc accttgttat atttagtata
     2041 tttccaatct tacctgacta atcgccgaca ctccaaatat attttctatt acctattgta
     2101 aaagtaaaaa aacccgccga tataccggaa aagtccggta aatccgcctt acccggacag
     2161 cgccgctaaa gaaattttcc ggcgcaaagg atttttttca aattattttt cgattccgga
     2221 ttggacgggc gaaattttac cgaagcttat atatattttt taaaacaaag acgataaaca
     2281 aatttttact tgaaaaaatt atttatcggg tatacaatcc agccacttct tttcatccgg
     2341 aaattttatg cagtttgtaa aaaaatcgtt ttaccgaccg cgaagccgtc cgcatcgcta
     2401 tcgcgcgtaa tcagaaatcg tcggtctccg acgctcgcgt gcagctgcat agtcgaatct
     2461 ccgggagcgg gaaaatttac cgttacacgt tcggcgatgt cggaaaaatt caaagcgaaa
     2521 tatgaattcc ggagcgcgag ccgtcgtttt attaataaaa ttaacgacgc cggcggcgcg
     2581 tttcggcaaa accgccgccg cgactcgtct atgtattgca gatacgattc aatgattttt
     2641 taaccgaacc tttcagaatg ccgaaaagca attttggcgg gtgatttcgt tagttacaga
     2701 ataaagccca atgttcgacg aaattgataa acatctggaa agctgggcga gagatgtttt
     2761 tgaaggcgga gacctcgaaa tatctttcgc cgcaccgctt gcggaaacgg aaaaaccgag
     2821 cgtttattta tacttgctcg acgttcttcc cacgccgccc ggtcgcggag ttcgtctgcc
     2881 gccgctccgg ataacgcttc gctatctggt cgtgccgcag gcgaaaacgc cgggcgaatc
     2941 gcaccgattg ctcggcaaaa tgcttgtttc ggcgatggaa aacgccgaat tcgaagttga
     3001 aaaagagcct ctctcgctcg acctgtggcg ggctttcgga atcgcgccgc gtcccgcgtt
     3061 tatcctgcgc gcggcattta aacacgatag aaaagaacaa atcgcaccgc ccgtgcgcga
     3121 acccgtccgg ctccggcagg cattgctgga gagcttgcag ggacaaattt cgcttaaccg
     3181 gattccgctc gtttccgcaa aggtcgaaat tcctttgctc aaagttttta cccagaccga
     3241 tgccgacggc aaattccgat tcgcctcgct gccgtccgag ccgcaggaca aaagcctgct
     3301 gattcgggca aaaggacgcg aattcagcgt ttcgaccggc gacgcgatac gccgcggcaa
     3361 cctgttttta attgacctaa aattggagga ttaaaatgcc gacttatcaa acaccgggaa
     3421 tttacattga agaaatttcc accggaaccc gtcctatcga agccgtcgga accagcacgg
     3481 caggctttgt cgggacgtcg cccaacgcgg acgcgcgcgt caacgaagcc tttgccgtca
     3541 acagctggtc gcagttcatt aaggaatatg ttgccgaaga cagcaaaagc acgccgcttt
     3601 cgcaggcggt tttcggtttt ttccagaatg gcgggagccg ctgttacgtc gtcaatatcg
     3661 gagcaaacgg ctcgctgctc ggcgacggga aacggcgaca gggactcggg cttttagaag
     3721 aaatcgacga ggtggcaatc gtcgccgcgc cgggctttac cgatacggct tcctacgacg
     3781 cgctgctcga acattgcgag cggatgcagg atcgcttcgc cattctcgac gcgcccgcta
     3841 cggtcagcag catcgacgcg ctgaaaaagg tcggcgctgc cggaaaaccc aaaaaaccgg
     3901 aaggcgaaac gggcgcgggc ggcgataccg gcggcgatac gggcggcgcg cgtccccgca
     3961 ccagcgaccg cggctacggc gctttttatt ttccgtggct tatcgtcaac gacccgtttt
     4021 cgccgggaga acccgtcatc gtgccgccgt ccgggcatct tgccggtatc tacgcccgca
     4081 ccgacgccac gcgcggcgtg cataaagcgc cggcaaacga aagcgtgcgc ggcgcgctcg
     4141 atttggctta tcgcatcacg caatccgaac agggcgagct gaacactctg ggcgtcaact
     4201 gcctgcggtt tttcccgacg caggggattc gcgtctgggg cgcgcgcacc ttagccgaca
     4261 gcgccagcga atggcgttat gtgcccgtcc gccgactgtt caatatgatt gaagaatcga
     4321 ttgcgcgcgg cacgcgctgg gtcgttttcg agccgaacga cgtgacgctc tggaaatcaa
     4381 tcgcgcgcga catcagagct tttctgacgc tgcaatggcg acagggcgcg ctgatgggcg
     4441 cgacggcgga agaagcgttt ttcgtccagt gcgacgcgga aacgaatccg cccgaagaga
     4501 ttgacgccgg acgggtcata gcggttatcg gcatcgcgcc ggtcaagccc gccgagtttg
     4561 ttattttccg tatcggacag agcgccgccg gaaccgaaat cgaaggagga atctagatat
     4621 gccgccgaaa gcagatgaag ccaaaccgca agccgcaccg gctaatgctt acgttgaccc
     4681 gtttcgcgcc tacaatttca agctggaaat tcagggcatc accgaagcgc attttacgga
     4741 agtcagcaat atggaagtca aggtcacgcc gattgcctac cgcgaggcgg gcaacagtca
     4801 ggtcgtgcat tacgttcccg gtcagaccga atacggagcc atcacgctgc gctacggctt
     4861 gacgcgctcg cgcgagctct gggactggtt tatgaccggc gtaaaaggaa aagtgcagcg
     4921 caaaaactgc tcgattatcg tgctcgacgc cgacggcgcg accgaagttc tgcgctggaa
     4981 cctgataaac gcctgggcgg cgcagtggcg cggcgcgatg ctgaacgctc tcgatcagga
     5041 agtcgccatc gaatcgctga cgctggtttg cgagactttc gaaagagctt aagagaaaca
     5101 aaacgatttt tgttttgcgt ttggcgctcg aatttttgtt ttgcgcccga ccattagtga
     5161 aaagctatgc gtttagtcga ttacaaaatt aaattcttcg atttcgtcgc gggtaatctc
     5221 cggcggctgg ctgagcttct gaccgtcgaa gccgcgccgg aacaggaaga ttccgcgccg
     5281 gagaaaacgc tctcgccgat ggaagactgg ctcgaaaaaa cgcgaaacct cccggctgaa
     5341 aactgggtcg atttttccgg cgcaaatcag cccgatgcgt ttcgggaaaa cgaatcggag
     5401 ccggaagccc agccgccgaa ttcatctttt gccgccgaga cgggcgcagc ggatttcgtt
     5461 ccggaaaaac cgaaaaaaca aacgccggtc gagacttttt tcaagaaaaa gcacgaagcc
     5521 gcagagaatc gtcaaacttt cggtaatctc gaagcttcaa ataaagcggc gagcaagacg
     5581 gaagtttccc gggcggaaaa gccgaaaaag acgaaacccg aatcgcgctt tttcagtatc
     5641 aaattttcgg accaaaagag aaaacaggaa aatgccgaac cgctcgagcc tttagccgaa
     5701 agcttcgctc aatcgacggc gaaagaccaa gaccggctgg aaaaggcaaa gcgaaatttc
     5761 cggctttttc cggctgaagc cgcttccgaa agcagcgcgg agaccgccgc gccgctcgtt
     5821 ataaatttta tcgaaaatca gccgccgaaa gctgaagctc ccgctttcga gcgaaaaaaa
     5881 tccgcggcgg taaaatttga aaaaatcgag ccggcaaagc cgaaaaacac aatcgaaaag
     5941 acggagtcaa tcgtcaaaaa atcgtctgcg cggagagaaa tttccaatca atttccgccc
     6001 gccgcgtttt cccgaaaaac gggcggcgaa tcagccgccg atataaattt tgagtttccg
//
LOCUS       DASWNE010000093         4865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_533067, whole genome shotgun sequence.
ACCESSION   DASWNE010000093 DASWNE010000000
VERSION     DASWNE010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533067"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VGB68_01915"
     CDS             complement(<1..270)
                     /locus_tag="VGB68_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0048012.1"
                     /translation="MGGILKITEVGGKSWELILLPNTVYSIGRAKDNDIVLNDQRVSR
                     RHAQIRSGENEWTLVDGYYVDGELKRSVNRVFVNGLPQFEKILESS"
     gene            354..1502
                     /locus_tag="VGB68_01920"
     CDS             354..1502
                     /locus_tag="VGB68_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY0048013.1"
                     /translation="MIYFDNNATTSPAPEVFEAMKPFLDEFYGNPSSGYTFGRAARAA
                     VEIAREQVAALTGANSVNEIVFTSGGTESDNWAILGALEAAPERRHIVTTRVEHEAVR
                     KLCEKLEDKNYAVTWLEVDENGFINLDELKNSLRRRDTAIVSAMLANNETGILFPVEE
                     IARVVKENSDALFHVDGVNAVGKIPFDLKSSEIDLFSLSGHKFHAPKGVGALYIKDGV
                     RLPSLLTGGGQENARRAGTEAVHQIAGLGKAAELAKDFSAMKKVCALRDKLEDKILQT
                     SPNAKLNGTSDRNFRLPNTSNISFPNLNGEAILAKLNDSGVCVSTGSACNAENHTASA
                     VLQAMNVPYSDAMGAIRFSLGRYNTETEVDFVLENLPKIIDELSSMAL"
     gene            complement(1489..2076)
                     /locus_tag="VGB68_01925"
     CDS             complement(1489..2076)
                     /locus_tag="VGB68_01925"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEY0048014.1"
                     /translation="MGLAKLKSKISVEDYLEGEKISPVKHEYVYGEIYAMAGTSDNHN
                     RIVGNLVNSLLNHLRDSSCEPFFGDIKVRVSPNVYYYPDVLVSCEENPENSYFRNQPI
                     LIVEVISPSTREIDRREKLLFYQQMPSVQEYVIVEQEKMHVEIHRRQPDGRWITYFYN
                     DNDLDEQLEFQSVGLKINLEEIYRRVKFDKKITEP"
     gene            2218..4677
                     /locus_tag="VGB68_01930"
     CDS             2218..4677
                     /locus_tag="VGB68_01930"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY0048015.1"
                     /translation="MINKIYLRFLLSLTLVVSIFAVSLPPVGYAQTSAQTQSSNDLAR
                     QLQTIEEKVEARRRELGIPGMSLAIVSDDRIIYAKGLGYKDFEKQVPVTADTQFAIGS
                     ATKAFTALSVLMTQDEGKLSLDDSPKKYLPYFKMKDAETDARITVRDLLSHSSGLNRT
                     DLAMITGKLNRAELIQVAGEAKPTARLREKFQYQNIMFAAAGEIVAQTQGKAWEKFIP
                     ERIFKPLGMTNSTMSMVEMQKVKDYSFGYDYNFDTKETRRLPFREIDQVAPAGSINSS
                     AKDMAQWLRFVLNGGSAGGKRLVSEKGFEEWLKPQMKISPNGKFNYSLGWFLQEWNGL
                     KVVQHGGNIDGFNALVAMIPEKKLGFVMLTNVSGSPLGNELMPIVWENILGKPNATTS
                     AAGTVAPEKEVGKYKFEAAGLDLEVKMENGKLVAVVPGQPTYVLENVGGRKYKLTNAP
                     EGFFITFREKDAYLEQPQGNYTLPRAVAEIINAAPNPADNPAKELIGKYETPSGKSAI
                     EVREADGKVSLVVPGQPPYELRVKDKDVFSSPLLPDAYSVKVTRDAGGKLSGIVLVQP
                     EGEFSFKYAGAGEKPNAPKMSLDEVMTKAVNALGGEANWRKISSRETKYDIDFEHQGV
                     KGTVTSYQKAPNLFASDTTFTALGKPIATAYEYFDGTRGGDVTSFSPADTLTGQRLED
                     VKVENDFYGLLNWKSGLKSAEVARIDKLGDEEVYVVVIRPEKASEYTYFISTKTFLPL
                     KKSGVIVSSTSSVKLPMSQTFSDYRAVDGVMIPYKTVTSTPTMGDVVSYVREVKHNVS
                     IDDAKFKPKAEIDGKVKAKNK"
BASE COUNT         1265 a         1209 c         1254 g         1137 t
ORIGIN      
        1 gctgctttcc agaatttttt caaactgcgg caaaccgttg acaaagacgc ggttaacgct
       61 gcgcttcaat tcgccgtcca cgtaatagcc gtcgaccagc gtccattcat tctcgcccga
      121 tcttatctgc gcgtggcggc gcgagacgcg ctggtcgttc aaaacgatgt cgttgtcttt
      181 cgccctgccg attgaataga cggtattcgg aagcagaatg agctcccaac tttttccgcc
      241 gacttccgta attttaagaa ttccgcccac aaattttgtt attggtaaat ggtcaatgat
      301 aaattgaagt ataaataatt taccatttcc cgcgcatcat ttaccactgt cttatgattt
      361 atttcgataa taatgcgact accagcccgg cccccgaagt tttcgaggcg atgaagccat
      421 ttctcgatga attctacggc aatccttcgt ccggctacac attcggacgg gctgcgcgcg
      481 cagccgtcga aatcgcgcgc gagcaggttg ccgcgcttac tggcgcaaat tcagtcaatg
      541 aaatcgtttt cacgtccggc ggaaccgaat ccgacaactg ggctatcctg ggcgcgctcg
      601 aagccgctcc cgaacggcga cacatcgtca ccacgcgcgt cgaacacgaa gcggtgcgca
      661 aattatgcga aaaactcgaa gataaaaatt atgcggtgac gtggctcgaa gtcgacgaaa
      721 acggctttat aaatctggac gaattaaaaa attccctgcg ccggcgggac acggcaatcg
      781 tttccgcgat gctggcgaac aacgaaacgg gaattttgtt tcccgtcgaa gaaatcgcgc
      841 gcgtcgtcaa ggaaaattcc gacgcgcttt ttcacgtgga cggcgtgaac gcggtcggaa
      901 aaattccgtt tgatttgaaa agctcggaaa tagatttgtt ctcgctttcc ggtcacaaat
      961 ttcacgcgcc gaaaggcgtc ggcgcgcttt atataaaaga cggcgtccgt ctgccttctt
     1021 tgttgaccgg cggcggtcag gaaaacgcgc gccgcgccgg aaccgaagcc gtccatcaaa
     1081 tcgccggttt gggcaaagcg gcggagcttg ccaaggactt ctccgcgatg aaaaaagttt
     1141 gcgcgctgcg cgacaagctc gaagacaaaa ttttgcagac gagtccaaac gccaagctca
     1201 acggaacttc cgaccggaat tttcgcctgc cgaacacttc caacatctcg tttccgaatc
     1261 tcaacggcga agcgattctg gcaaagctga acgattcggg cgtctgcgtc tcgaccggct
     1321 cggcttgcaa cgccgaaaat cacacggcgt cagccgtttt gcaggcgatg aacgtcccgt
     1381 attcggacgc gatgggcgcg attcgctttt ctctgggaag atacaatacg gaaacggaag
     1441 tcgatttcgt tttggaaaat ttaccgaaaa ttattgatga attaagttct atggctctgt
     1501 gattttttta tcgaacttca cgcggcgata gatttcttcc agatttatct tcaagccgac
     1561 cgattgaaac tcaagctgct cgtccagatc attatcatta taaaaataag ttatccagcg
     1621 tccgtccggc tggcggcgat ggatttcgac gtgcattttt tcctgctcga cgatgacgta
     1681 ttcctgaacg ctcggcattt gctggtaaaa cagcagcttc tcgcggcggt cgatttctct
     1741 ggtcgaaggc gagataacct cgacaatcaa aatcggctgg ttgcgaaagt aagaattttc
     1801 gggattttcc tcacacgaaa cgagaacgtc cgggtaataa taaacattcg ggctgacgcg
     1861 aactttaata tcgccgaaaa aaggctcgca cgaagaatcc cgaagatgat tcaataaaga
     1921 attaacgaga tttcccacaa tccggttgtg attgtcgctg gtgccagcca tcgcgtaaat
     1981 ttcgccgtaa acgtattcgt gtttgaccgg actgattttt tcgccttcca gataatcttc
     2041 gacactgatt tttgatttta gtttcgctaa tcccataact gcgttaattg taaatcgaaa
     2101 ccggagaatc gcaaacatgg atttttcgcg ttaaattttt cttcggcggc aaaactttat
     2161 agcggcgctc agcgtaatta agcttgaagt tttttccaac tctccaggag gacagtcatg
     2221 ataaacaaaa tttatttgcg atttttactc tcgctgacgc tggtcgtttc gatttttgcg
     2281 gtttctttgc cgccggtcgg ctacgcccag acttccgcgc aaactcagag ctctaatgat
     2341 ttagcccgac agcttcagac gattgaagaa aaagtcgaag cgcggcgcag ggaactcggc
     2401 attcccggaa tgtcgctggc aatcgtcagc gacgaccgga ttatttacgc caaaggtctc
     2461 ggctataaag actttgaaaa acaggttccg gtgaccgccg acacgcagtt tgccatcggc
     2521 tcggcgacaa aagctttcac ggctttgtcg gttttgatga cgcaggacga agggaaactc
     2581 agcctcgacg acagcccgaa aaaatatctg ccgtatttca aaatgaagga cgcggagacg
     2641 gacgcgcgca ttaccgtgcg cgatttgctt tcgcattctt cgggattgaa tcgcacggat
     2701 ttggcgatga taaccggaaa gctcaatcgc gccgagttga ttcaggtcgc gggcgaagcg
     2761 aaaccgacgg cacgcctgcg cgaaaaattc cagtatcaga acataatgtt tgcggcggcg
     2821 ggcgagattg tcgctcagac gcaaggtaag gcttgggaaa aattcattcc cgaacgcatt
     2881 ttcaaaccgc tcggaatgac gaacagcacg atgtcgatgg tcgagatgca gaaagtcaaa
     2941 gattattctt tcggctacga ttacaatttc gatacgaaag aaacgcggcg gctcccgttt
     3001 cgcgaaatcg accaggtcgc gcctgccggc tcgataaatt cgagcgcgaa agatatggcg
     3061 caatggcttc gttttgtttt aaacggcggc agcgcgggcg gcaagcgtct ggtttccgaa
     3121 aagggttttg aagaatggct caagccgcag atgaaaattt cgccgaacgg caaattcaat
     3181 tacagcctgg gctggttttt gcaggaatgg aacggtttga aagtcgtcca gcacggcgga
     3241 aacatcgacg gtttcaacgc gctggtggcg atgattccgg aaaagaaact cggctttgtg
     3301 atgctgacga atgtttccgg ctcgcctctg ggcaacgagc tgatgccgat tgtctgggaa
     3361 aatattttgg gcaaaccgaa cgcgacgacg agcgcagcgg gaacggtcgc gccggaaaaa
     3421 gaagtcggaa aatataaatt tgaagccgcc ggtcttgacc tcgaagtaaa gatggaaaac
     3481 ggcaagctcg tcgccgtcgt tccgggtcag ccgacctacg ttctggaaaa cgtcggcgga
     3541 cgcaaatata aattgaccaa cgccccggaa ggatttttca ttactttccg cgaaaaagac
     3601 gcgtatctcg aacagccgca gggcaattac actctgccga gagcggtcgc cgaaattata
     3661 aacgccgctc cgaatcccgc cgataatccg gcgaaggaat taatcggcaa atacgaaacg
     3721 ccgagcggca agagcgcgat tgaagtcagg gaagcggacg gcaaagtgtc gctggtcgtg
     3781 cccggtcagc cgccttatga gctgagagta aaggacaaag acgttttcag ctcgccgctg
     3841 ctgcccgacg cttattcggt aaaggtcacg cgcgacgcgg gcggcaagct gtcgggcatc
     3901 gttctggttc agcccgaagg cgaattttcg tttaaatacg cgggcgcggg cgaaaaaccg
     3961 aacgcgccga aaatgtcgct cgacgaagtg atgacgaaag ccgtcaacgc gctcggcggc
     4021 gaggcgaact ggcgaaaaat ttcttcgcgc gagactaaat acgacatcga ctttgaacat
     4081 cagggcgtca aaggcacggt cacgagctat caaaaagcgc cgaatctttt cgccagcgat
     4141 acgacgttta ccgcgctcgg caagccgatt gcgacggctt acgaatattt cgacggcacg
     4201 agaggcggcg atgtcaccag cttttcgcca gccgacactc ttaccggaca gcgcctcgaa
     4261 gacgtcaaag tcgaaaacga tttttacggc ttattaaact ggaaaagcgg tttgaaatcg
     4321 gcggaagtcg ccaggataga taaactcggc gacgaagaag tttacgtcgt cgtgattcgt
     4381 cccgaaaaag ccagcgaata cacttatttc atctcgacca aaacgtttct gcctttaaaa
     4441 aaatccggcg tcatcgtttc cagcacttcg agcgtaaaac tgccgatgtc gcagactttc
     4501 agcgattacc gcgccgttga cggagtgatg attccctaca aaaccgtaac ctcgacgcct
     4561 acgatgggcg atgtcgtgtc gtatgtcagg gaagttaaac acaatgtttc gattgacgat
     4621 gcgaaattta agccgaaagc cgagattgac ggcaaagtga aagcgaagaa caagtaactg
     4681 ctcgccgaaa aatcacgtca aaagaaggtg aagtttatgg cttcaccttt tttgtttgac
     4741 ggaaaattgc ttaaaattat tttgaaagct ttaacaggcg atatttatga aattgttatt
     4801 ttgtcccctg attttaatat tcgctttttc gggcgcggtt tttgcccaga ccggcaaagg
     4861 catcg
//
LOCUS       DASWNE010000094         1776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_38546, whole genome shotgun sequence.
ACCESSION   DASWNE010000094 DASWNE010000000
VERSION     DASWNE010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38546"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1427)
                     /gene="radA"
                     /locus_tag="VGB68_01935"
     CDS             complement(45..1427)
                     /gene="radA"
                     /locus_tag="VGB68_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEY0048016.1"
                     /translation="MAKNPATIYVCQNCGHQARKWLGRCPDCNEYNTFVEERFRPSAQ
                     AAGKGSIVSGLRLVRETKPISYGEIESQDDARTSSGIDEFDRVLGGGIVPGSLVLIGG
                     SPGIGKSTLVAQVADKLSANNLKVLYISGEESERQIKMRGERLGLNAENLFLLPETNL
                     EAILAETDKMKPDIIIVDSIQTVFSPNIESAPGSVSQVREVAGQFMIFAKQTATPVFL
                     IGHVTKEGSIAGPKALEHIVDTVLYFEGDRHHNHRIIRATKNRFGAANEIGVFEMTGE
                     GLIAVGNPSEVFLQERPEGASGSVVTVCMEGTRPMLVEIQALVSGSKYGTGRRMAQGF
                     DQNRTALLIAVLEKRLGFQLMGDDVFINIAGGLEVDEPAADLGIIAAIASSYRNLPIP
                     ADVAVFGEVGLTGEVRGALQAQVRAREAQTLGFKKLILPATNKTGLEKLLGTRVVGVR
                     NLDEALDELF"
     gene            complement(1495..>1776)
                     /locus_tag="VGB68_01940"
     CDS             complement(1495..>1776)
                     /locus_tag="VGB68_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048017.1"
                     /translation="SFSEFFLMYDRTIPSVLILFIGLFISFFLISSYKKKISPEKTAL
                     SEVLQLLRETSNIIAKQENWSVLSRAEFNIRLSRFNLEEKSKSAFTFFS"
BASE COUNT          452 a          458 c          426 g          440 t
ORIGIN      
        1 cctttcttta tttttgtgca aagccattgt cgcgagcttt gagtttaaaa caattcgtcc
       61 agcgcctcgt ccagattgcg gacgccgacg acgcgcgtgc cgagcagttt ttccaagccg
      121 gttttattcg tcgcgggcaa aatcaatttt ttaaagccga gagtttgcgc ttctctggct
      181 ctgacctgcg cttgcagagc gccgcgcacc tcgccggtca agccgacttc gccgaacacg
      241 gcaacgtcag ccggaatcgg cagattccgg tagctcgaag caatcgccgc aatgatgccc
      301 aaatccgccg ccggttcgtc gacttccaaa ccgcccgcga tgtttataaa aacgtcgtcg
      361 cccatcaatt gaaagccgag acgtttttcc agaacggcaa tcaaaagcgc cgtgcgattc
      421 tggtcaaagc cctgcgccat ccgtctgccg gttccgtatt tcgagccgga aacgagcgcc
      481 tgaatttcga ccagcatcgg gcgcgtgcct tccatacaaa ccgtgacgac cgacccgctc
      541 gcgccttccg gtcgctcctg caaaaaaact tccgacgggt tgccgaccgc gattaagcct
      601 tcgcccgtca tttcaaaaac gccgatttcg tttgccgcgc cgaaacgatt tttcgtcgcg
      661 cgtataatgc ggtgattgtg atggcggtcg ccttcaaaat acaaaaccgt atcgacgatg
      721 tgttcgagcg ctttcggtcc ggcaatcgag ccttctttcg tcacgtgacc gataagaaaa
      781 acgggcgtcg cggtctgctt ggcgaaaatc ataaactgcc cggcgacttc gcgcacctgc
      841 gatacggaac cgggcgcgga ctcgatattc ggcgaaaaaa ccgtctgaat cgaatcgacg
      901 atgataatat ccggcttcat tttatcggtc tccgccagaa tcgcttcgag attcgtttcc
      961 ggcagaagaa aaagattttc ggcgtttaag ccaaggcgct cgccgcgcat cttgatttga
     1021 cgctcggatt cctcgccgga aatatacaac actttcaaat tattggcgct caatttatcg
     1081 gcgacctgcg cgaccaatgt cgattttccg attccgggcg agccgccgat taaaaccagc
     1141 gagccgggaa cgattccgcc gcctaaaacc cggtcgaatt cgtcgatgcc ggagctggtt
     1201 cgggcgtcgt cctgcgattc aatctcgccg taggaaatcg gttttgtctc gcgcaccagt
     1261 cgcagaccgg aaacaatcga gcctttgccg gcagcctgcg ccgacggacg aaagcgttct
     1321 tcgacaaaag tattatattc attgcagtcg ggacatctgc cgagccattt gcgagcctga
     1381 tgaccgcagt tttgacagac gtaaattgtt gccggatttt ttgccatcaa ttgattttaa
     1441 ctgaaaaaca atcgcggaca aatacagctt ttagaagatt tttacaatcg aaaattatga
     1501 gaagaaagta aaagcggatt ttgatttttc ttctaaatta aagcgcgaaa gtctgatatt
     1561 aaattctgca cgagaaagaa ctgaccaatt ttcttgttta gcgataatat ttgaggtttc
     1621 acgtagaagt tgtaaaactt ccgaaagagc tgttttttca ggagaaatct tttttttgta
     1681 gcttgaaata aggaaaaaag agataaacag tcctataaaa agtattaata ctgagggaat
     1741 agttcggtca tacattagga aaaattcaga aaaaga
//
LOCUS       DASWNE010000095         3606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_74292, whole genome shotgun sequence.
ACCESSION   DASWNE010000095 DASWNE010000000
VERSION     DASWNE010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74292"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VGB68_01945"
     CDS             complement(<1..196)
                     /locus_tag="VGB68_01945"
                     /inference="COORDINATES: protein motif:HMM:NF015546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048018.1"
                     /translation="MSLVIDFPNPRFSEDIEGVICVSETEYLNPENLLYAYRQGIFPW
                     MVEDYPYVPWFCPPARAVLEF"
     gene            266..601
                     /locus_tag="VGB68_01950"
     CDS             266..601
                     /locus_tag="VGB68_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048019.1"
                     /translation="MEKLVNFAKSLLTNRLGIVLATLNLCYFVSGNFIGAHFSPGNFD
                     KLMLSQNAPAIALCFVPNRLIHFLFPQTDRLWQITMVFLLSFVALQWLFIGWAAKMIA
                     RKLMKLMAR"
     gene            complement(662..2026)
                     /locus_tag="VGB68_01955"
     CDS             complement(662..2026)
                     /locus_tag="VGB68_01955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEY0048020.1"
                     /translation="MREALFGSQRDFTTGSIGQAIFILSVPMIMEMFGESLFALVDIF
                     FVGSLGASAVAVVGLTESMMALIFAVAIGLSMGATATVARRIGEKDAAGAAKSAAHVI
                     YLGVIVSLVMSAIGIVFAPTFLRLLGGTPQVVEQGTTFTRIMLGGNAVVVFLFLLNAI
                     FRGAGDASIAMRVLWLANLLNMILAPSFIYGFAFFPELGVTGAAVGTTIGRGCGVLFA
                     AWYLFRPGGRIHVDRESWRLDVGLLWKIVKLSSTAVLQFLIATASWSALIIIIAGFGD
                     EAIAGYVIALRVVMFALLPSVGLSNAAATLVGQSLGAGKPERGEKAVWKAAYINAAFL
                     GVIGLIFLIFASPIVRIFTPDPQVLAYGTQALQIIAYGFVFYGFGMVLETAFNGAGDT
                     WTPTYLNLFIFWIFEIPLAYVLAYYFGLGAAGAFWAITIAFSLLAVVSAILFNRGKWK
                     LKTV"
     gene            complement(2353..2472)
                     /locus_tag="VGB68_01960"
     CDS             complement(2353..2472)
                     /locus_tag="VGB68_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY0048021.1"
                     /translation="MKTDVLIVGVRPTGPAPAGQLVRYGVDFVIFDKKSRPAK"
     gene            complement(2620..3294)
                     /locus_tag="VGB68_01965"
     CDS             complement(2620..3294)
                     /locus_tag="VGB68_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048022.1"
                     /translation="MAAPPPLLPADDLPPLAAAPDEDVEPFDAPPLPLLPELFAALPP
                     PDEAFDFVVLPALPPAPFVPELPDPAFDEPPLSLPEAPPLDFAVAPALPVPFVPASPL
                     VFFAPPVDDEPALLAFDDEALLLLLLPEPALLDFEESPLFAFVDFPALPVPFVPASPP
                     SITPLTASATTPKTPIAAPCAAPLRISPAASLALSKISVVTLFLADLLPPLFDFAEVD
                     FEPVDF"
BASE COUNT         1020 a          923 c          914 g          749 t
ORIGIN      
        1 caaactccaa aacggcacgc gcgggcgggc aaaaccacgg cacatacggg taatcttcga
       61 ccatccacgg aaaaattccc tgccggtaag cgtaaagcag attttcggga ttaagatatt
      121 ccgtttcgct gacgcaaatg acgccttcga tgtcttcgct aaaccgcgga ttgggaaaat
      181 cgattacaag tgacattttt ttctgcaaca aatttcatca gccgatgtgt aaaaaataac
      241 aaatctgtca ggcgaggtaa agaaaatgga aaaacttgtg aattttgcca aaagtttatt
      301 gacgaaccgt ttgggaatcg ttttagcgac gctcaatctt tgttatttcg tgtcgggcaa
      361 ttttatcggc gcgcattttt cgccgggtaa tttcgacaaa ctaatgctca gccaaaacgc
      421 tccggcgata gctctttgtt ttgtgcctaa ccggcttata cactttcttt tcccgcagac
      481 ggaccggctc tggcagataa cgatggtttt ccttttatct ttcgtcgcgc tgcaatggct
      541 tttcatcggc tgggcggcga aaatgattgc ccgaaaattg atgaaactaa tggcgcggta
      601 aaagcaataa ggcggaagac ggtagaaatt tatcttttga cttccgcctt ctgccttttt
      661 atcacaccgt tttcagtttc cattttccgc gattgaaaag aatggcggaa acgaccgcca
      721 ggagcgaaaa cgcaatcgta atcgcccaga aagcgcccgc cgcgcccaag ccgaaataat
      781 acgccagaac gtaggcgagc ggaatctcga atatccagaa gataaaaaga ttcagataag
      841 tgggcgtcca ggtgtcgccc gcgccgttaa acgccgtttc cagaaccatt ccgaaaccgt
      901 agaagacaaa gccgtaagcg ataatctgta aagcctgcgt gccgtaagcc agaacttgcg
      961 ggtcaggcgt gaaaattcgg acaatcggac ttgcgaatat caggaaaatc aaaccgatga
     1021 cgccgagaaa cgccgcgttg atataagccg ctttccagac cgctttttcg ccgcgttccg
     1081 gcttgcccgc gcctaagctt tgaccgacca gcgtcgccgc cgcattgctc aagccgaccg
     1141 acggcagaag cgcgaacatc acgacgcgca gcgcgatgac gtatccggca atcgcttcgt
     1201 cgccgaaacc ggcgatgata atgatgagcg cgctccagct tgccgtcgca atcagaaatt
     1261 gcagcacggc ggtcgaagaa agcttgacga ttttccacaa aagcccgacg tcgagccgcc
     1321 agctttcgcg gtcgacgtga atgcgcccgc ccgggcgaaa cagataccaa gccgcgaaca
     1381 aaacgccgca gccgcgcccg atggtcgtac ctaccgccgc gcccgtcacg cccaattcgg
     1441 ggaaaaacgc gaagccgtaa ataaagctcg gcgcgagaat catattcagc agatttgcca
     1501 gccacaatac gcgcatcgca atcgaagcgt cgcccgcgcc gcggaaaatc gcgttgagca
     1561 gaaacagaaa aacgacgacc gcgtttccgc cgagcataat gcgcgtaaac gtcgtgccct
     1621 gctcgacgac ctgcggcgtt ccccctaaaa gccgcagaaa agtcggcgcg aaaacgatgc
     1681 cgatggcgct cattaccagc gagacgataa cgccgagata gataacgtgc gccgccgatt
     1741 tcgccgcgcc tgccgcgtct ttttcgccga tgcggcgggc gacggtcgcc gtcgcgccca
     1801 tacttaaacc gattgcgacg gcgaaaatca aagccatcat tgattcggtc agaccgacca
     1861 ccgccaccgc cgacgctccc aaactgccga caaagaaaat atcgaccagc gcaaacaggc
     1921 tctcgccgaa catttccata atcatcggaa cggacaaaat gaaaatcgcc tgcccgattg
     1981 aaccggtcgt aaaatcgcgt tgcgagccga aaagagcttc acgcaaaata gaccaaaaag
     2041 aattattctc cgtctgcaag acagagtctt ccgaagtttg cgacatataa aaaaccttta
     2101 ttgtatttat gaaacgaacc cgaagcgaga tttgttcgtc cggaatccaa gcgaacgaaa
     2161 caaacaggaa atttgcggag tgtttacagg acaagtattt tcataatttt gagaagatac
     2221 cataaggtcg gataaaattt caatcgccga aaagttattt ttatttaaat tctttcgata
     2281 tgattttata attttttttt cgtgtttgcc ctgttcgacc ggcaaaacga acggatactt
     2341 gctcaggcgc gcctatttcg ccggacgcga ttttttatca aaaataacga aatcgacgcc
     2401 gtatctgaca agttgtccag ccggcgccgg ccccgtcggt cggacgccga cgataaggac
     2461 gtctgttttc atatattttc cctcaaaacc ataagtataa ccacgatcaa aaaaattgtt
     2521 tatttttccg caacacggtg ttaaagttat agctgaaata caagtttttt caatcattta
     2581 agggaggaaa aattatttta tgccagctaa aaaagacagt cagaaatcga ccggctcaaa
     2641 atcgacttcg gcaaaatcaa aaagcggcgg aagcaaatcc gctaaaaaga gtgtgacgac
     2701 cgagattttg gacaaagcca aagacgccgc gggcgagatt ctgagcggtg cggcgcaggg
     2761 cgctgcaatt ggagttttgg gcgttgtcgc cgacgctgtg agcggtgtaa tagaaggcgg
     2821 tgacgccgga acgaaaggca ccggcagcgc cggaaaatcg acgaaagcga aaagcggcga
     2881 ttcttcaaaa tccaaaagcg caggctcggg cagcagcagc agcagtaaag cttcgtcgtc
     2941 aaaagccaaa agcgcaggct cgtcgtcaac aggcggcgca aagaaaacga gcggtgacgc
     3001 cggaacgaaa ggcaccggca gcgccggcgc aacggcaaaa tctaaaggcg gagcttccgg
     3061 cagcgataaa ggcggttcgt cgaaagccgg atcaggcagc tccggaacga aaggcgcggg
     3121 cggcagcgca ggcaaaacga cgaaatcgaa agcctcgtca ggcggcggca gcgcggcgaa
     3181 aagctccggc agtagcggca gcggcggcgc gtcgaaaggc tcgacgtctt cgtccggcgc
     3241 ggcggctaaa ggcggtaaat cgtcggcggg caaaagcggc ggcggcgcag ccaaaagcgc
     3301 aaagacaaaa agtaaataat ttcgctttcg gttaaattga tttaacgaaa atttgctcgc
     3361 gaaagcggcg aaaaagtgaa accgaacgga tttgagattt caaatctgaa atctcaaatc
     3421 gcaggcttcg attgccggtc tgcggcttca ttttctattt cgcacgcttt cgcgagcagg
     3481 ttttcgtatt ttttataact tgccgcctgc cggacaattc cttcttgact tgcggctttg
     3541 cacaaaaata aagaaaggga aaattgtaaa ttgtaaattg taaattctaa attgatgttc
     3601 ggtatc
//
LOCUS       DASWNE010000096         3984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_38645, whole genome shotgun sequence.
ACCESSION   DASWNE010000096 DASWNE010000000
VERSION     DASWNE010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38645"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VGB68_01970"
     CDS             <1..334
                     /locus_tag="VGB68_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048023.1"
                     /translation="RGSEAIYAVGLKGEFVVTVKIGGDGKVISADSGNIRPLLLKQVL
                     EESAGKWLFSADKDSKVREAKIIFEYRTKFENSKKNSDNPPTITSKFKKPMRFIITGE
                     FHRRTSVY"
     gene            324..1736
                     /locus_tag="VGB68_01975"
     CDS             324..1736
                     /locus_tag="VGB68_01975"
                     /inference="COORDINATES: protein motif:HMM:NF005560.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0048024.1"
                     /translation="MSINQTKSIAILGSGFLGLTLALRLAESGNQVTVFESAAEIGGL
                     ASAWQIGDVVWDKHYHVTLLSDTYTRKIVAELGLENEFKWVETKTGFYTDGELVSMSN
                     AVEFLKFPPLDLIGKFRLGATIFYASRVKDWKNLEKIPVEQWLTKLSGRRTFEKMWKP
                     LLKAKLGEAYRETSAAFIWATIQRLYAARNSGLKKEMFGYVSGGYARVLQRFGEVLRE
                     KGVRVRLNTKVEKIEKTEGDKIKVFAAESTRGADAFREKIKINSFQNLSELSVLSVSP
                     AANSAASEFDSVILTCPASVAAKICPQFSDTEKAKLENVKYQGIVCASVLMKTSLSDF
                     YVTNITDETPFTGIIEMSALVDKAEFGGNALVYLPKYVAPTDELFEKSDAEIEETFLD
                     ALEKMYARFNRKDVLAFKVSRVRQVFPIPTLDYSKNLPDSKTSLENVFVINSAHIVNG
                     TLNVNETVQLAEKFFRQLKT"
     gene            1744..2061
                     /locus_tag="VGB68_01980"
     CDS             1744..2061
                     /locus_tag="VGB68_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048025.1"
                     /translation="MTAYYHKTQNEVLVGDRVEFKNWLFFWRGWQAGRVYYVPGISPK
                     NEELERDGLSWISIENKTGEQIGVLVEPETFQIQKSVRFVGRAEDGFTETPANYYFGD
                     NDD"
     gene            2079..3089
                     /locus_tag="VGB68_01985"
     CDS             2079..3089
                     /locus_tag="VGB68_01985"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY0048026.1"
                     /translation="MKKQIASLSLDLDNKWSYMKTHGDAGWERLPSYLDACVPRVLRF
                     LKERNLNITFFIVGQDAALDKNAEAIAQISKENHEIGNHSFRHEPWLHLYTKQELIDE
                     FQKTEEALEKVTGQMPKGFRGPGYSLSPTVLEVLSERDYEYDCSTLPTYIAPLARAYY
                     FFKSPEMSDEEREKRKKLFGKFADGFQSLKPYKWRIGEKSLIEIPVTTLPVFKTPIHA
                     SYVIYLSTFSKPLARTYWKTAIEMCRRTGTQLSLLLHPLDFLSGEDAPELKFFPAMNL
                     PAEEKLEFLSEILEALTGKFEVVDMRRHAREAAKQNLRVIEAKPETQKRASVKEISRE
                     TI"
     gene            3076..>3984
                     /locus_tag="VGB68_01990"
     CDS             3076..>3984
                     /locus_tag="VGB68_01990"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HEY0048027.1"
                     /translation="MKQFEMIQPAGEAEKAAGVSFSRTIRALVRSLRPREWVKNLLVF
                     SGLIFSQSLLDAHNVLFSFGGFIAFCLASSGVYLFNDLCDLEKDRLHPVKKNRPLASG
                     ALNVNLARLAMILLFAGAVVLAFQLSSAFALAIGFYLALNLAYSLRLKHAVILDVIII
                     SSGFVLRAVAGALVLQVEVSAWLVLCTSMVALLVGFGKRRHELVLLQENAGSHRASLN
                     DYSVEFLDAMMTICGGAAVLTYALYTMAEDTVSRFNTHNLLITLPFVVYGVFRYLFLV
                     HKRKEGGDPVQLLFRDRASVINIVLWI"
BASE COUNT         1039 a          934 c         1032 g          979 t
ORIGIN      
        1 gagaggctcg gaagcaattt atgctgtcgg tttaaaaggc gaatttgtcg taactgtaaa
       61 aattggtgga gacggaaaag taatttcagc agacagtgga aatattcgtc cgcttctttt
      121 gaaacaagtg ttggaagagt cagctggtaa atggcttttt tcggctgata aagattctaa
      181 agtaagagaa gcaaagataa tttttgaata cagaacaaaa ttcgagaatt ctaaaaagaa
      241 tagcgacaat ccaccgacaa taaccagtaa atttaaaaag ccaatgcgtt ttattataac
      301 gggagaattt catcgaagaa caagtgtcta ttaatcaaac aaaatctatc gccatcctcg
      361 gaagcggttt tctcggttta acgctggcat taagacttgc cgaaagcgga aatcaagtta
      421 ccgttttcga atccgccgcg gaaatcggcg gtttggcgag cgcgtggcaa atcggcgacg
      481 tcgtctggga caaacattat cacgtcacgc ttttgtcgga cacttacacg cgaaaaatcg
      541 tcgccgagct gggtttggaa aatgaattta aatgggttga aacaaaaacg ggattttata
      601 ccgacggcga gctcgtttcg atgtctaacg ccgtggaatt tctcaaattt ccgccgctcg
      661 atttaatcgg caaattccgt ctgggcgcga cgatttttta cgcctcgcga gttaaagact
      721 ggaaaaatct ggaaaaaatt ccggtcgaac aatggctgac gaagctttcc ggcaggcgaa
      781 cgtttgaaaa aatgtggaag ccgcttttga aagcgaagct aggcgaagct tatcgcgaaa
      841 cttccgccgc gtttatctgg gcgacgattc agagattata cgcggcgcga aattccggct
      901 tgaaaaagga aatgttcggc tacgtgagcg gcggctacgc gcgcgttttg caaagattcg
      961 gcgaagtttt gcgcgaaaaa ggcgtccgcg ttcgcctgaa tacgaaagtc gaaaagattg
     1021 aaaaaacgga aggcgataaa ataaaggttt ttgccgcaga aagcacacgc ggcgcggacg
     1081 cgttcagaga gaaaataaag ataaattctt tccaaaatct ttccgaactc tcggttctct
     1141 ccgtatcgcc cgcggcaaat tccgccgctt ctgaatttga ttccgtaatt ttaacctgcc
     1201 cggcaagcgt cgcggcgaaa atctgcccgc aattttccga tacggaaaaa gcgaagctcg
     1261 aaaacgtaaa atatcagggc atcgtctgcg cctcggtgct gatgaaaacg tcgctttccg
     1321 atttctacgt cacaaacatc acggacgaaa cgccttttac cggcattatc gagatgtcgg
     1381 cgctcgtcga taaagcggaa ttcggcggaa acgcgctggt ttatttaccg aaatacgtcg
     1441 cgccgacgga cgaattattc gaaaaatccg acgccgaaat cgaagaaacg tttttagacg
     1501 cgctcgaaaa aatgtatgct cgctttaacc ggaaagacgt tctggctttc aaagtttcgc
     1561 gcgtccggca ggttttcccg attccgacgc tcgattattc gaaaaatctg ccggattcga
     1621 aaacctctct ggaaaacgtt tttgtgatta actcggcgca catcgtcaac ggaactttga
     1681 acgtcaacga aaccgtccag ctggcggaaa agttttttcg gcaattaaaa acgtaaaaag
     1741 cagatgacgg cttactatca caaaacacaa aatgaagttt tggtcggcga ccgcgtcgaa
     1801 tttaaaaact ggctgttttt ctggcgcggc tggcaagccg gaagagttta ttatgttccg
     1861 ggaatatcgc cgaaaaatga agaattggaa cgcgacggat tgagctggat ttcgattgaa
     1921 aataaaacgg gcgagcaaat cggagttttg gtagagcctg aaacttttca aattcaaaaa
     1981 agcgttcgtt tcgtcggtcg cgccgaagac ggatttaccg aaacgcccgc taattactat
     2041 ttcggcgata atgacgattg accggataat tttgcgctat gaaaaaacaa atcgcgagtc
     2101 tttcgctcga cctcgacaat aaatggtctt atatgaaaac gcacggcgac gcgggctggg
     2161 agcggcttcc gtcttacctg gatgcgtgcg tgccgcgcgt tttgcgtttt ttgaaggaaa
     2221 gaaatctgaa tattacgttt tttatcgtcg ggcaggacgc cgcgctggat aaaaacgcgg
     2281 aagcaatcgc gcaaatctca aaggaaaatc acgaaatcgg caatcacagt tttcgccacg
     2341 agccgtggct gcatctttac acgaaacagg aattgattga cgaatttcaa aaaaccgagg
     2401 aagcgctgga aaaagtcacc ggacagatgc cgaaaggctt tcgcggtccc ggctacagcc
     2461 tttcgccgac ggttctggaa gttttgagcg agcgcgatta cgaatacgac tgctcgaccc
     2521 tgccgactta tatcgcgccg ctggcgcgcg cttattattt ttttaaatcg ccggaaatgt
     2581 cggacgaaga acgcgaaaaa cgcaaaaagc tgtttggcaa gttcgcggac ggctttcagt
     2641 ctctgaagcc gtataaatgg cgaatcggcg aaaaatcgct aatcgagatt ccggtcacga
     2701 ccttgcccgt tttcaaaacg ccgattcacg ccagctacgt gatttatctt tcgacgtttt
     2761 ccaaaccgct cgccagaact tactggaaaa cggcaatcga aatgtgccgc cggacgggaa
     2821 cgcagctgtc tttattgctt cacccgctcg attttctgag cggcgaagac gcgcccgaac
     2881 ttaaattttt cccggcgatg aatttaccgg cggaagaaaa gctggagttt ctgagcgaga
     2941 ttttagaggc tttgacaggc aaattcgaag tcgtcgatat gcgtcgtcac gcgcgggaag
     3001 cggcgaaaca aaacctgcgc gtaatcgaag ccaagccgga aacgcagaaa cgcgcgagcg
     3061 taaaggaaat cagccgtgaa acaatttgag atgatacagc ccgccggcga agcggaaaaa
     3121 gccgcgggcg tttcgttcag ccggacgata cgcgccctgg ttcgctctct gcgcccgcgc
     3181 gaatgggtaa aaaatctgct cgttttcagc ggtctgatat tttcccaatc cttgctcgac
     3241 gcgcacaacg ttttattcag cttcggcggt tttatagcgt tttgtctggc aagcagcggc
     3301 gtttacctgt tcaacgattt atgcgatttg gaaaaagacc gcctgcatcc ggtgaagaaa
     3361 aatcgcccgc tcgcttccgg cgctttgaac gttaatctgg cgcggctggc gatgattctg
     3421 ctttttgccg gcgcggtcgt tctggctttt caattaagct cggcgtttgc gctggctatc
     3481 ggcttttatc tggcgctgaa tctggcttat tcgctccggc tcaaacacgc cgtcattctc
     3541 gacgtaatta ttatttccag cggttttgtt ctgcgcgccg tggcgggcgc tctggttctg
     3601 caagtcgagg tttcggcgtg gctggtgctc tgcacttcga tggtcgcgct gctggtcggt
     3661 ttcggcaagc ggcgtcacga gcttgttctt ttgcaggaaa atgcgggctc gcatcgcgcg
     3721 agtctgaacg attattccgt cgaatttctc gacgcgatga tgacgatttg cggcggcgcg
     3781 gcggtgctga cttacgcgct ctacacgatg gcggaagata cggtcagccg cttcaatacg
     3841 cataatctgc tcatcacgct gccgtttgtc gtctacggcg tttttcgcta tctgtttctg
     3901 gtgcacaagc gcaaggaagg cggcgacccg gttcaattgc tgtttcgcga ccgcgcctcg
     3961 gttattaata tcgtgctctg gatt
//
LOCUS       DASWNE010000097         6042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_285838, whole genome shotgun sequence.
ACCESSION   DASWNE010000097 DASWNE010000000
VERSION     DASWNE010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6042
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285838"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..1511)
                     /gene="sufD"
                     /locus_tag="VGB68_01995"
     CDS             complement(189..1511)
                     /gene="sufD"
                     /locus_tag="VGB68_01995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEY0048028.1"
                     /translation="MTSTAEETIYSNYFRESLLQNQPEWLTELREKAFRFFTENGFPT
                     PLDEEWKYTNVAPLAQENFVLPDASAAAVALNSRDALAPFVFDESKESVLVFVNGKFD
                     KSLSNLSALGGAAALSFPEAAGSERFSEIFKTKLANQVDFDKNGFTALNTAFIDEGAF
                     LFVPKAAKVETPIQFLFLTEDGKISLPRVLIVAENFSEATIIETYVRSEEASYLTNAV
                     VEISLADEAKIKHYRVQRESHAAFHIGTSAAEIRRGSIYDATNITLGARLSRHDVSVK
                     FLTTGGEAFVDGLYMVGGEQHADTHSVIDHQLPNCVSHQTYKGIVDGKARAVFNGKVF
                     VRENASGTDAQQSNKNLLLSNDARVDTKPQLEIFNDDVKCSHGATVGQLEEEELFYLL
                     SRGLNESLARNLLTYGFAEEIVNKIGIETIKRQLDEAVLNRLNANLEA"
     gene            complement(1532..2134)
                     /locus_tag="VGB68_02000"
     CDS             complement(1532..2134)
                     /locus_tag="VGB68_02000"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEY0048029.1"
                     /translation="MDDALRIILPLYLVAFFGVAFVWRSVAVWKQTGVNPYVLGKTDN
                     AHDFIGVVFRLTFALIVAVIIVFAFFSPLYQYAAPIAWLEHRPVKSIGLVLLVASLVW
                     TAIAQIQMGASWRVGIDRKNPTGLVENGLFGVSRNPIFFGMRVALLGFFLTLPNAVTL
                     LALVLGDVLMQIQVRLEEEFLRSSHGAKYEQYCRGVRRWI"
     gene            complement(2140..2892)
                     /gene="sufC"
                     /locus_tag="VGB68_02005"
     CDS             complement(2140..2892)
                     /gene="sufC"
                     /locus_tag="VGB68_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEY0048030.1"
                     /translation="MLLEIKNLHAGIEGKEILKGLNLQINKGEVHAIMGPNGSGKSTL
                     AKVLAGHPSYEVMQGEVLYEGKNLLELEPDERAKEGIFLAFQYPVEVPGVSNSQFLRL
                     AYNQKMKHLGLEELDPLEFNDYLKEKAKVVEMSSDFFKRSVNEGFSGGEKKRNEILQM
                     AVLEPKLAVLDETDSGLDIDALRIVAGGVNKLRDAEKAIILVTHYQRLLNYITPDFVH
                     VLANGKIVKQGGKELALELEEKGYDWVKAAGN"
     gene            complement(2897..3667)
                     /locus_tag="VGB68_02010"
     CDS             complement(2897..3667)
                     /locus_tag="VGB68_02010"
                     /inference="COORDINATES: protein motif:HMM:NF021631.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY0048031.1"
                     /translation="MTDEIRKELLRLERERDIKILYAVESGSRAWGFASRDSDWDVRY
                     IYVHRLEWYLRIEDEKDSQEEILPNDVDLAGWELRKALRLFRKSNPPLMEWLNSPIVY
                     LQNFSTADRLRELTGKFFNPKSGLHHYLHMAEGNYREYLQRDLVRVKKYFYVLRPVLA
                     CDWIRQTGAMIPMEFRKLVETQVTDEKIADEIGKLLSRKIAGDELKQEPKIEILNEFL
                     ERKIKFYNDYVSRIERPEKPETALLDELFRETIFEVWK"
     gene            complement(3664..4281)
                     /locus_tag="VGB68_02015"
     CDS             complement(3664..4281)
                     /locus_tag="VGB68_02015"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEY0048032.1"
                     /translation="MKLAIVIFDKFTDLDLFLMWDLLNRVRLPDWSVKILGEAQAHVS
                     ATGISVATQGRIEEANDADAVLFVSGQGTRAKMIDKDWLSRFRLNPERQFIGSICSGA
                     LILAALRLLDGKTATTYPTSKAALEAFGVEVLEKPFIANGRVATAGGCLAQQYLIGWV
                     VENLADSDWRDLILKSIQPVGEGLYFADAENLTRLYAPQAKAISK"
     gene            complement(4283..5071)
                     /locus_tag="VGB68_02020"
     CDS             complement(4283..5071)
                     /locus_tag="VGB68_02020"
                     /inference="COORDINATES: protein motif:HMM:NF014609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEY0048033.1"
                     /translation="MNLQIFQVDAFTKKPLSGNPAAVCPLQEWLPDETMLKIAAENNL
                     SETAFFVKRDEHYEIRWFTPTIEIDLCGHATLATAFVIFNCLKLEEREIKFYSSRSGH
                     LSVEKQDERLILNFPKYGLNEIETLLDLTAAIGKTPEKMWETQGNMAMLLFETEEDIK
                     SLQPDMSALVKLAFDEIIVTAKGTDADFVSRLFAPRIGIPEDPVTGATHCSLIPYWAE
                     RLGKEKLYARQLSSRGGELFCELNGERVRIGGNAALYLKGEIYI"
     gene            complement(5088..5399)
                     /locus_tag="VGB68_02025"
     CDS             complement(5088..5399)
                     /locus_tag="VGB68_02025"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0048034.1"
                     /translation="MKIFRSREFTGKRAWESIAVADMNGITTRIHWTDEPYKWHTNDG
                     EEVFAVLDGSVEMHYRENGALKTVELKTGDVFFAEIGCEHVAHPLGEARILVIEKKGS
                     V"
     gene            complement(5396..5638)
                     /locus_tag="VGB68_02030"
     CDS             complement(5396..5638)
                     /locus_tag="VGB68_02030"
                     /inference="COORDINATES: protein motif:HMM:NF016837.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034257 - nicotinamide riboside
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0034258 - nicotinamide riboside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEY0048035.1"
                     /translation="MQYYGIDWLATVCGLTGVYMLGNKNKYGFVFFMLASLSWFLFGI
                     LTASVAMSIGSFIFFTMHLRGWLNWNRESRKLENLQ"
     gene            complement(5645..>6042)
                     /locus_tag="VGB68_02035"
     CDS             complement(5645..>6042)
                     /locus_tag="VGB68_02035"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY0048036.1"
                     /translation="LLEKDMEKIQIEGAPAPAGHYSHGIVHNGFVFVSGQLPRDAVTG
                     EVETGAIEAQTELALRNVEAVLRAAGTDLNHVVQMTIYVSEMELWDAVNATYARILGE
                     HRPARAIVPVKDLHFDTKIEIQAIAALKE"
BASE COUNT         1418 a         1682 c         1375 g         1567 t
ORIGIN      
        1 ggcgttttga ttaataattt cgatgtcgcg caggttcaat tccttgcgat tttctctcgc
       61 tacataatca cgcaaagccg attcgacaat ctccgaacgc ttatgttttt ttcctgccaa
      121 agcatcaact tttagcagca attctttcgg caatgtgact gatgttctaa ctctcatatt
      181 tttaaattct atgcttccag attcgcgttc aagcgattca aaaccgcttc gtccaattgt
      241 cttttgatgg tttcaatccc gattttgttc acaatttctt cggcgaaacc ataagtcagc
      301 agatttcgcg ccagcgattc gttaagcccg cgcgaaagaa gataaaacaa ttcttcttct
      361 tccagctgcc cgacggttgc gccgtgcgag catttcacgt cgtcgttaaa gatttcgagc
      421 tgcggttttg tatcgacgcg cgcgtcgttc gacaaaagca gatttttatt cgattgctgc
      481 gcgtccgtcc cgctcgcgtt ttcgcggacg aagactttgc cgttaaaaac cgcgcgcgct
      541 ttgccgtcga cgattccctt gtaggtttga tgcgaaacgc agttcggcaa ttggtggtcg
      601 atgaccgaat gcgtgtcggc gtgctgctcg ccgccgacca tataaagccc gtcgacaaaa
      661 gcttcgccgc cggtcgtcag aaattttacc gaaacgtcgt gacgcgaaag ccgcgcgccg
      721 agcgtgatat tggtcgcgtc gtaaatcgag cctcggcgaa tttccgcggc gctcgtgccg
      781 atgtgaaaag ccgcgtggct ttcgcgctga acgcgataat gcttgatttt cgcttcgtcg
      841 gcgagcgaga tttcaacgac cgcattcgtc agatagctcg cctcttcgct tcgcacgtaa
      901 gtttcgataa tcgtcgcttc gctgaaattt tcggcgacga ttaaaactct cggcagcgaa
      961 atttttccgt cttcggtcaa aaataaaaac tggattggag tttcgacttt cgccgctttc
     1021 ggaacgaaca aaaacgcgcc ttcgtcgata aaagccgtgt ttagagcggt aaatccgttt
     1081 ttgtcaaaat cgacctgatt cgccagtttc gttttgaaaa tttcggaaaa tctttcgctg
     1141 ccggcggctt ccggaaagct caaggctgcc gcgccgccga gcgccgaaag attcgataag
     1201 cttttatcaa actttccgtt gacgaaaacc aaaacgcttt ctttcgattc gtcgaaaaca
     1261 aacggcgcga gcgcgtcgcg cgaatttaaa gcgacggctg ccgcggacgc gtccggcaag
     1321 acaaaatttt cctgcgcgag cggcgcgacg ttcgtgtatt tccactcctc gtcgagcggc
     1381 gtcggaaagc cgttttccgt aaaaaaccgg aaagcctttt cgcgcagctc cgtcagccat
     1441 tcgggctgat tttgcaataa gctttctcta aaataattgc tgtaaattgt ttcttctgct
     1501 gttgaagtca ttttataaat ctctgttatt tttaaatcca gcggcgcacg ccccggcaat
     1561 attgctcgta tttcgcgccg tgcgagcttc ttaaaaactc ttcttccagg cgcacctgaa
     1621 tctgcattaa aacgtcgccc aaaaccagcg ccagtaaagt aaccgcgttc ggcaaggtca
     1681 ggaaaaatcc cagcagagcg acgcgcattc cgaaaaaaat cgggttgcgc gaaacgccga
     1741 aaagcccgtt ttcgaccagc ccggtcggat ttttccggtc gatgccgacg cgccacgacg
     1801 cgcccatctg aatctgcgcg attgccgtcc aaacgagtga cgcgacgagc aggaccagcc
     1861 cgattgattt caccggtcga tgttcgagcc aggctatcgg cgcggcgtat tgataaagcg
     1921 gcgaaaagaa cgcgaagacg attatcaccg cgacaatcaa agcgaaagtc agccgaaaaa
     1981 cgacgccgat aaaatcgtgc gcgttgtcgg ttttgcccag aacatacgga tttacgccgg
     2041 tctgtttcca gaccgcgacc gagcgccaga cgaaagcgac gccgaaaaac gcgaccagat
     2101 acaaaggcaa aattattcgc agagcgtcgt ccattcgatt tagtttcccg ccgctttcac
     2161 ccaatcgtag cccttttctt caagctccag cgccagttcc ttgccgccct gcttgacgat
     2221 ttttccgttt gccagaacgt ggacgaaatc cggcgtgatg taattcaaaa ggcgttgata
     2281 atgcgtcacc agaattatcg ctttttccgc gtctctcaac ttattgacgc cgcccgcgac
     2341 gattctgagc gcgtcgatgt caagccccga atcggtttcg tccagcacgg cgagcttcgg
     2401 ctccaggacc gccatctgca aaatctcgtt gcgttttttt tcgccgccgg aaaaaccttc
     2461 gttgaccgag cgcttgaaaa aatccgaact catttcgacg actttcgctt tctctttcag
     2521 ataatcgtta aattccagcg ggtcgagttc ttcaagcccg agatgtttca tcttctggtt
     2581 gtaggcgagc cgcaaaaact gcgaattcga cacgcccgga acttcgaccg ggtattgaaa
     2641 cgcgagaaaa atgccttctt tcgcgcgttc gtcaggctct aattcaagca gatttttgcc
     2701 ttcgtaaagc acttcgccct gcatcacttc gtagctcgga tgacctgcca gaacttttgc
     2761 cagagtcgat ttgcccgagc cgttcggtcc cataatggcg tgaacttcgc ctttattaat
     2821 ctgtaaattc aagcccttta aaatctcttt gccttcgatt ccggcgtgta aatttttaat
     2881 ttccagtaac atatttttat ttccaaactt caaaaatcgt ttctctaaat aactcgtcga
     2941 gcaaagcggt ttccggtttt tccgggcgtt cgattcggct gacgtagtcg ttgtaaaact
     3001 ttatctttcg ctccaaaaac tcgttcaaaa tttcaatttt cggctcctgc tttaattcgt
     3061 cgcccgcgat ttttctcgac agcaatttcc cgatttcatc cgcgattttt tcgtccgtca
     3121 cctgagtctc gaccagtttc cgaaattcca tcggtatcat cgcgcccgtt tgtctaatcc
     3181 aatcgcaagc caaaaccggg cgcagaacgt agaaatattt ttttacccgg accaaatctc
     3241 tctgcaaata ttcgcggtaa ttgccttccg ccatatgcag gtaatgatgc aaacccgatt
     3301 tcgggttaaa aaatttcccg gtcagctcgc gcaatctgtc ggcggtcgaa aaattttgga
     3361 gataaacaat cgggctgttc agccactcca tcaacggcgg attcgacttt ctgaaaaggc
     3421 gcagagcctt tctcaattcc cagcccgcca ggtcgacgtc gttcggcaaa atctcttcct
     3481 gagagtcttt ttcgtcttcg attctcaaat accattcgag ccggtggacg taaatgtacc
     3541 tgacgtccca atcgctgtcg cgcgaggcaa atccccaggc gcggctgccg ctttcgacgg
     3601 cgtataaaat ctttatgtcg cgctcgcgct cgagccgcaa aagctctttc ctgatttcat
     3661 ccgtcattta gaaatcgctt tcgcctgcgg cgcgtaaagc cgcgttaagt tttccgcgtc
     3721 ggcgaaatat aagccttcgc cgaccggttg aatcgacttc aaaatcaaat cgcgccaatc
     3781 gctgtcagcc agattttcaa ccacccagcc aatcagatat tgctgcgcca gacaaccgcc
     3841 ggcggtggcg acgcgtccgt ttgcaataaa cggtttttcc aaaacttcga cgccgaacgc
     3901 ttcgagcgcg gctttcgagg tcggataagt cgtcgcggtt tttccgtcga gcaaacgaag
     3961 cgcggctaaa atcaaagcgc ccgaacaaat cgagccgata aactgccttt cgggattcag
     4021 acgaaaccgc gaaagccaat ctttatcaat cattttcgcg cgcgtgccct gcccgctgac
     4081 gaacaagacg gcgtcggcgt cgttggcttc ttcgattcgc ccttgcgtcg ccaccgaaat
     4141 tcccgtcgcc gaaacgtgcg cttgcgcttc gcctaaaatt ttcaccgacc aatccggaag
     4201 gcgaacgcgg ttgagcaaat cccacatcag aaacaagtcg aggtcggtaa atttatcgaa
     4261 aataacgatt gccagtttca tatcagatat aaatttcgcc ttttaagtaa agcgccgcat
     4321 tgccgcctat acggacgcgc tcgccgttta attcgcaaaa caattcgccg ccccggctgg
     4381 aaagctggcg cgcgtagagc ttttcctttc cgagcctttc agcccaatac ggaatcagcg
     4441 agcaatgcgt cgcgcccgtc acagggtctt cgggaatgcc gatgcgcggc gcgaaaagac
     4501 gcgaaacgaa atcggcgtcc gtcccttttg ccgtgacgat gatttcgtca aaagccagtt
     4561 tgacgagagc cgacatatcg ggctgcaaag atttaatgtc ttcttccgtt tcaaacagaa
     4621 gcatcgccat attgccctgc gtttcccaca ttttctcggg cgtcttgccg atggcggcgg
     4681 ttaaatcgag caaagtttcg atttcattca gaccgtattt cggaaaattt aaaatcaagc
     4741 gctcgtcctg tttttcgacg ctcaaatgcc cgctgcgcga cgaataaaat ttgatttctc
     4801 tttcttccag cttcagacag ttaaaaatca cgaaagccgt cgccagcgtc gcgtgaccgc
     4861 aaaggtcaat ctcgatggtc ggcgtaaacc accgaatctc gtaatgctcg tctcttttca
     4921 caaaaaaagc cgtctcggaa agattatttt ccgccgcgat tttcagcata gtttcgtcag
     4981 gcagccattc ctgcaaagga caaaccgccg ccggatttcc gcttaagggc tttttcgtaa
     5041 aagcatcgac ctgaaaaatt tgaagattca taaatttcga ttctctatta aacgctgcct
     5101 tttttctcga ttactaaaat tctcgcttcg ccgagcggat gcgcgacgtg ttcgcagccg
     5161 atttccgcaa aaaaaacatc gcccgttttt aattcgaccg ttttgagcgc gccgttttcg
     5221 cgataatgca tttctacgct gccgtccaaa acggcgaaga cttcttcgcc gtcgttcgtg
     5281 tgccacttgt agggctcgtc agtccagtga attcgcgtcg tgatgccgtt catatcggcg
     5341 acggcaatcg attcccaggc gcgctttccc gtaaattccc tgctgcgaaa aatcttcatt
     5401 gcaaattttc cagtttgcgg ctttcccggt tccaattcag ccagccgcgc aaatgcatcg
     5461 taaagaaaat gaagctgccg atagacattg cgacgctggc ggtcaaaatc ccgaacagaa
     5521 accagctcaa actggcgagc ataaagaaaa cgaaaccgta tttattttta tttccgagca
     5581 tataaacgcc cgtcaaaccg cagacggtcg ccagccagtc tatcccgtaa tattgcattt
     5641 ttctttactc tttaagcgcc gctatcgcct gaatctcaat tttcgtgtca aagtgcaaat
     5701 ccttgaccgg cacgattgcc cgcgccggac gatgctcgcc cagaatgcgc gcgtaagtcg
     5761 cgttgaccgc gtcccagagc tccatctccg aaacgtaaat cgtcatctgg acgacgtgat
     5821 tcaaatccgt tccggcggcg cgcaaaaccg cttcgacgtt tcttaaagcg agctcggtct
     5881 gcgcttcaat cgcgcccgtt tccacttcgc ccgtcaccgc gtcgcgcggc aattgcccgg
     5941 acacaaaaac gaaaccgttg tgaacgattc cgtgcgaata atgtccggcg ggcgccggcg
     6001 cgccttcaat ttgtattttt tccatatctt tttctaacaa tc
//
LOCUS       DASWNE010000098         4339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_38769, whole genome shotgun sequence.
ACCESSION   DASWNE010000098 DASWNE010000000
VERSION     DASWNE010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38769"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..473)
                     /locus_tag="VGB68_02040"
     CDS             complement(168..473)
                     /locus_tag="VGB68_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEY0048037.1"
                     /translation="MAATTAKKRLTQTEIVNQLAEKSGLKKAQAKEFFNALAALAASE
                     VKKNGEFTVPGFGKLVKATRKAREGRNPATGDVIKIPAKTTVKFRIGKALKDTIGGK"
     gene            complement(594..670)
                     /locus_tag="VGB68_02045"
     tRNA            complement(594..670)
                     /locus_tag="VGB68_02045"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(634..636),aa:Pro,seq:tgg)
     gene            complement(735..1340)
                     /gene="rdgB"
                     /locus_tag="VGB68_02050"
     CDS             complement(735..1340)
                     /gene="rdgB"
                     /locus_tag="VGB68_02050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEY0048038.1"
                     /translation="MEIVIATKNAGKVKELAGLLADLPVNLRSLNDFENIVEPEETGV
                     DFIANAVLKARDYARQTGLWVVADDSGLEVEALDNAPGVFSARYAGENATGEERINKL
                     LSELNKTGNENRRARFVCAMAVADDRGEIRYVAEGDCGGSIVDTPKGTNGFGYDPVFV
                     PEGFSETFGELSDEIKREISHRAHASKKIIAYLRHFIAVST"
     gene            1456..2844
                     /locus_tag="VGB68_02055"
     CDS             1456..2844
                     /locus_tag="VGB68_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048039.1"
                     /translation="MGKLILDVSTTSTTTTFNPASLSTTSAADENVFSAPAVSARELG
                     FELGLWLSGLESFLQIRNHSFAGDSGNRAKTSNRDWTKEFRLVHSTLLLCSKLTLQLS
                     KTIRDKNFAASDERIDLIPDLNTIRFEFSDAEGLSQLASALVDSILLNEGLLRAAPLR
                     FSEWTAWSNFLSAKLKAAEVSGKLIAAAEKEGENFLPKKLKDLLENKPIPFALEADLR
                     LVLPRFAKILKWLDAIGQMHDRDEPLKPAMLLFARINEQTQEMMRFINNRLLRFKNEE
                     DSLFAALDCAVYAASIELRKVYNFELIGFAEARQSPLIFARVETAHGLLNDCFQQTLV
                     NFAQLIEPDVKATELFPNLQTKLQQSLTLREDLWRVLQIVRKAEKNSESVSLQKLHTK
                     LTDFLNSTIKYLFYKDLETVERFIEEVLNTQQKSELVSILHRFGAYLETLLGQVNMRV
                     VLSNYPFNPPQE"
     gene            complement(2912..>4339)
                     /locus_tag="VGB68_02060"
     CDS             complement(2912..>4339)
                     /locus_tag="VGB68_02060"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY0048040.1"
                     /translation="LLFSLLPLLQIRNIKPNLLLRDENNEKLRRLDWTRLIFAFVCLA
                     GLLGLAIWQAGSIKVGAAFLGGLAVTSLILYLAAAALTSALKKTKKFSSFAVGQAVNS
                     LYRPGNQTRVVLLAVGLGAFVVLSVQSLQANLVREFDFTRNQKLPSLFLVDIQRSQVE
                     KLKQIAEEATGEAQHPIPTVRGRIALVNGEPFDYEQREVRQQQGQIGREFAVTYRPNL
                     EENESLSAGEWWENASSPDAEVSVEERMSNTLKLSVGDVITFDILGRRINAAVRNIRK
                     IDVRNSRTAFVFVFRPGVLEKAPQTFVLPITKQLAAAERARFQRSVLDNFPNIQILDT
                     ADIIKTITRLIENFVLAISFVGSFVMLTGILILIGSVALTKSQRIYENAILKTLGAKR
                     LTLTTILFTEYAVLGVLAGIIGALFSTALSFAVTKYILKINWEFDFGLTLTGIFATAF
                     IVMLIGVLASFGVIFKKPLAVLRAQ"
BASE COUNT         1057 a         1111 c         1076 g         1095 t
ORIGIN      
        1 cttttgtttc ctgcatgaaa atagaatcgt aacaaaattt tttccttttg tcaccatttt
       61 ttttagaagt ttgtaacatt tttctcaagc ggcaaaaacg gaaaaaagac cggcaagccc
      121 aactgctgct tgccggtcaa aaaagtatta aatttatact taaaatatta ttttccgccg
      181 atagtatctt tcagggcttt gccgatgcgg aatttgacag tggttttggc tgggattttg
      241 atgacgtcgc cggtcgccgg gttacgtcct tcgcgcgctt tacgcgtggc tttcaccaat
      301 tttccaaagc cgggcacggt aaattctccg ttctttttga cttcgcttgc cgcgagagcc
      361 gccagagcgt tgaagaactc tttcgcctga gcttttttca agccggattt ttccgccaat
      421 tgattgacga tttcagtttg tgtcaaacgc ttctttgcag tagtagctgc cataattaag
      481 ttttacctcc aaaagttttt agtgatattg caataaccta agttaaaatt tttgctcgta
      541 gtttgcaaac aatgaaagga gtatacgcag tcggcttcca acaaaaatac aattggtcgg
      601 ggcgagatga ttcgaacatc cgacctctcg gtcccaaacc gagcgcacta ccaggctgtg
      661 ctacgccccg caacgtgcgt ttcattgaag aaacatcaac aagtaacgga ttttacgggc
      721 gaaaaatgcg aaggtcaagt tgaaacagcg ataaaatggc gcaaatacgc aattattttt
      781 tttgatgcgt gcgcgcgatg acttatttcg cgctttattt cgtcggagag ttcgccgaag
      841 gtttccgaaa agccttcggg cacgaaaacc gggtcgtaac cgaagccgtt cgtgcctttc
      901 ggcgtgtcga cgatgcttcc gccgcagtcg ccttcggcga cgtatctgat ttcgccgcgg
      961 tcgtcggcga cagccatcgc gcacacgaat ctcgcccgcc ggttttcgtt tccggttttg
     1021 ttcaattcgc tcaaaagctt gttgattctt tcttcgccgg tcgcgttttc gcccgcgtat
     1081 ctggcggaaa aaacgcccgg cgcattatcg agcgcctcga cctccagacc ggaatcgtcc
     1141 gccacgaccc aaagtccggt ttgccgcgca taatcgcgcg ctttcaaaac cgcgttggcg
     1201 ataaaatcga cgccggtttc ttccggttcg acgatgtttt caaaatcatt caagcttcgc
     1261 agattgacgg gcaaatccgc cagaagcccg gcgagttctt ttacttttcc ggcatttttc
     1321 gtggcgatta ctatttccat aaacccgata gttttgttaa aacgcttttt tttcttgcca
     1381 aactgcttat cgaaaaggta taaaattaat agcggcgttt ttcactcacg ccttttgacc
     1441 ttcgaattct tcgatttggg aaaactaatc ttggacgttt caaccacttc tacaacaact
     1501 acattcaatc cggcaagttt gtcaacgacg agcgcggcgg acgaaaatgt gttttccgcg
     1561 cctgcggtct ccgcccgcga gctcggtttt gagctcggat tatggctttc cgggctggaa
     1621 agctttctgc aaattagaaa tcactcgttt gcgggcgatt ccggaaaccg cgcgaaaacg
     1681 tcaaaccgcg actggacaaa ggaatttcgt ctggttcatt cgacgcttct tttgtgttcg
     1741 aagctcacct tgcagctttc caaaacaatc cgggacaaaa attttgcggc gagcgacgag
     1801 cggattgatt tgatacccga tctgaacacg atacgatttg aattttccga tgccgaagga
     1861 ctatctcagc tcgcctcggc gctggtcgat tcgattctgc tcaacgaagg cttgctgcgc
     1921 gccgcgccgc tcagattcag cgagtggacg gcgtggagca attttctttc ggcaaagctg
     1981 aaagcggcgg aagtttccgg caaattgatt gcggcggcgg aaaaagaagg cgaaaatttt
     2041 ctgccgaaaa aattaaagga tttgctggaa aacaagccga ttccgtttgc gctggaagcg
     2101 gatttgcgcc tggttctgcc gcgtttcgcc aagattttaa aatggctcga cgccatcggg
     2161 cagatgcacg accgcgacga gcctctgaag ccggcgatgc tgctttttgc gcgcatcaac
     2221 gagcagacgc aggaaatgat gcgctttatc aacaatcgcc tgctgcgctt taaaaacgag
     2281 gaagattcgc ttttcgccgc gctcgactgc gctgtctacg ccgcttcgat tgagcttcgc
     2341 aaagtttata atttcgagct catcggcttt gccgaagcgc gtcaatcgcc gcttattttc
     2401 gcgcgcgtcg aaaccgcgca cgggctttta aacgactgtt ttcagcagac tttagtgaat
     2461 ttcgcgcagc tgatcgagcc ggacgtcaaa gcgaccgagc ttttcccgaa tctgcaaacc
     2521 aaattgcagc agtctttaac tttgcgcgaa gatttgtggc gcgttttgca gatcgtgcgc
     2581 aaagccgaga aaaattccga aagcgtgtcg ctgcaaaaac tgcacacaaa gctgaccgat
     2641 tttctgaaca gcacgataaa atatttgttt tacaaggatt tggaaacggt cgagcgcttt
     2701 atcgaagaag ttttaaacac gcagcaaaaa tccgagctgg tctcgattct gcaccgcttc
     2761 ggcgcttatc tggaaacgct tctcggacaa gtcaatatgc gcgtcgtatt gtcaaactac
     2821 ccgtttaacc cgccgcagga atagaagaaa cgcggaatgc ggaaagcgga acgattaatt
     2881 gcaattaatc gttccgcttt ccgcattttg cttattgcgc gcgcaaaacc gccaaaggtt
     2941 ttttgaaaat tacgccgaag cttgccagaa cgccaatcag catcacgata aaggcggtcg
     3001 cgaagatgcc ggtaagcgtc aagccgaaat cgaattccca attgattttc agaatatatt
     3061 tcgtcacggc gaaagacaga gcggtcgaga acagcgcgcc gataattccc gccagaacgc
     3121 cgagcacggc gtattccgta aataaaatcg tcgtcaaggt aagacgcttc gcgccgagcg
     3181 tttttaaaat cgcgttttcg tagattctct gcgatttggt cagcgcgacc gagccgatta
     3241 gaatcaaaat tccggtcagc atcacgaagc tgccgacgaa ggaaatcgcc agcacgaaat
     3301 tttcaatcag cctggtgatg gttttgatga tgtccgccgt gtcgagaatt tggatgttcg
     3361 gaaaattgtc cagcacgctg cgctgaaacc gcgcgcgctc ggcggcggcg agctgtttgg
     3421 taatcggcag aacaaaagtc tgcggagctt tttccagaac gcccggacga aaaacgaaaa
     3481 caaaagccgt ccgcgaattg cggacgtcga ttttgcggat gtttctgacc gccgcgttga
     3541 tgcggcgtcc gagaatgtcg aaagttatca cgtcgccgac cgaaagcttc agagtattgc
     3601 tcattctttc ttcgacggaa acctcggcgt ccggcgaaga cgcattttcc caccattcgc
     3661 ccgccgacag gctttcgttt tcttccagat tcgggcgata cgtgacggcg aattcgcgcc
     3721 cgatttgacc ttgctgctgc cgaacttcgc gttgttcgta atcgaaaggc tcgccgttta
     3781 ccagcgcgat tcgcccgcgc acggtcggaa tcggatgctg cgcttcgccg gtcgcttctt
     3841 cggcgatttg ctttaatttt tcgacctggc tgcgctgaat atcgaccaga aacaggctcg
     3901 gcagtttttg attgcgcgta aaatcgaatt cgcgcaccag atttgcctgt aaagactgaa
     3961 cggagagcac gacaaatgcg ccgagcccga cggcaagcag cacgacgcgc gtttgattgc
     4021 cggggcgata cagcgaattg acggcttgcc cgacggcgaa cgacgaaaat ttctttgtct
     4081 ttttcaaggc ggaagtcagc gccgccgccg ccagatacaa aatcagagaa gtcacggcta
     4141 aaccgcctaa aaacgccgcg ccgactttta tcgagccggc ttgccagatt gccagcccga
     4201 gcagtccagc cagacaaaca aaggcgaaaa tcaaacgcgt ccaatcgagc cgccgcaatt
     4261 tttcgttgtt ttcgtcgcgc agaagcagat tcggcttaat attgcggatt tgcaaaagcg
     4321 gcagaagcga aaacagaag
//
LOCUS       DASWNE010000099         3362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_250703, whole genome shotgun sequence.
ACCESSION   DASWNE010000099 DASWNE010000000
VERSION     DASWNE010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250703"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1416
                     /locus_tag="VGB68_02065"
     CDS             <1..1416
                     /locus_tag="VGB68_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HEY0048041.1"
                     /translation="LADLHTPVSAFVRVCGESERAFLFKSIEGGERLARYSFIAANPY
                     LTVRARGKQTIIEQTGEAEKTENTPVFDYLRRHFNHNKLSRRANLPPLCGGAVGFLTY
                     DAVRFFEPVLDDGKIFEKDDAVWMFFRNVLAFDRLKQRIEIVSVVFTDEAAGDAESLR
                     ELYEIAVAETERIEKLLNQTAVFPQNAAPLEEKKNQVRSNWAKKDFLEAVETIQEKIL
                     AGDAYQIVLSQKFQRQISAEPVNIYRALRTMNPSPYMFFIKTGREILIGASPEMLVRC
                     RGRKLEYRPIAGTRKRGETETEDWILGEDLRSDEKEVAEHVMLVDLGRNDLGRVAEYG
                     SVEVDELMKIERYSHVQHLVTSLKARLREGFDRFDALASCFPAGTVSGAPKVSAMRII
                     DRLEPTARGIYAGTVGYIDYAENLDTCIAIRTIEVENGWANVQAGAGVVADSVPEKEY
                     EETINKARALMKAIEMAENGL"
     gene            1519..2340
                     /locus_tag="VGB68_02070"
     CDS             1519..2340
                     /locus_tag="VGB68_02070"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEY0048042.1"
                     /translation="MKLKIYFVLVCLVFVAACGGSSGDTAGSPEMTKNMLKLRGFEFN
                     EDGFFKAIRLNDTVAIKGFFDAGMEPNTKNARGETALTFAVGNAESKTVKAVAEKADI
                     NMQDNLGQSPLHLALSKQKDELFDYLLEKNADVNVGGAKGNLRNQTVLYLAVTRGRED
                     LVQKLLDKGADPNIADSDGAIPLSEACIGSRLSENIVKMLLDKGAKVNNQEKNGATPL
                     IYIASNKQASAENRRAVAQMLLGAGADKKLRDKDGKTALDWANKVGNKDLVDLLK"
     gene            2436..3002
                     /locus_tag="VGB68_02075"
     CDS             2436..3002
                     /locus_tag="VGB68_02075"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEY0048043.1"
                     /translation="MLLVIDNYDSFTYNLVQYLGELGAQMQVRRNDEVTIEEIENDLK
                     PERILISPGPGNPDEAGVSLKVLESFSEKLPILGVCLGHQAIGQFFGGKVVRAPVPVH
                     GKYVEVCHDGAGVFASLPYRFKAARYHSLIVERAGLPPELEVSATSPDGLIMGLRHRK
                     LKVEGVQFHPESILTSEGKKLLQNFLNL"
     gene            3113..>3362
                     /locus_tag="VGB68_02080"
     CDS             3113..>3362
                     /locus_tag="VGB68_02080"
                     /inference="COORDINATES: protein motif:HMM:NF014884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048044.1"
                     /translation="MPNTEWLVAAPMNATGSKADTPLHPFLARLMRGENLSFKEAAKF
                     FEALTDEDANPAQIAGALVALAAKGETFEELAGMAKAMR"
BASE COUNT          845 a          856 c          931 g          730 t
ORIGIN      
        1 ctggcggatt tgcacacgcc ggtcagcgct ttcgtgcgcg tctgcggcga atcggaacgg
       61 gcttttttat tcaaatccat cgaaggcggc gagcgcctgg cgcgctattc ttttatcgcc
      121 gcgaatcctt atctgaccgt ccgcgcgcgg ggaaaacaga cgattatcga acagaccggc
      181 gaagccgaaa agacggaaaa cacgcctgtt ttcgattatc tgcgccggca tttcaatcac
      241 aacaagctgt cgcggcgagc aaatcttccg cctttgtgcg gcggcgccgt cggatttctg
      301 acttatgacg cggtgcgttt tttcgagccg gttctggacg acggcaaaat ttttgaaaaa
      361 gacgacgccg tctggatgtt ttttcgcaac gttctggcgt ttgaccgcct gaaacagcgc
      421 atcgaaatcg tctccgtcgt ttttacggac gaagccgcgg gcgacgcgga aagcttgcgc
      481 gagctgtatg aaatcgcggt cgccgaaacc gaaagaatcg aaaagcttct gaatcaaacg
      541 gcggttttcc cgcaaaatgc cgcgccgctc gaagaaaaga aaaatcaggt gcgctcgaac
      601 tgggcgaaaa aagattttct cgaagcggtc gaaacgattc aggaaaaaat tctcgccggc
      661 gacgcttatc aaatcgtttt gtcgcagaag tttcaacggc agatttcagc cgagccggta
      721 aatatttatc gcgctctgcg cacgatgaat ccttcgccgt atatgttttt tataaaaacc
      781 ggcagagaaa ttttaatcgg cgcttcgccg gagatgctcg tgcgctgccg cggcagaaaa
      841 ctcgaatatc gaccgattgc cggaaccaga aaacgcggcg aaaccgagac ggaagactgg
      901 attctgggcg aagatttgcg ctcggacgaa aaggaagttg ccgaacacgt gatgctcgtc
      961 gatttggggc gcaacgattt ggggcgcgtc gccgaatacg gctcggtcga ggttgacgaa
     1021 ttgatgaaaa tcgagcgtta ttcgcacgtc cagcacctgg tgaccagcct gaaggcgaga
     1081 ttgcgcgagg gctttgaccg attcgacgcg ctcgcctcgt gttttcccgc cggaacggtt
     1141 tccggcgcgc cgaaagtcag cgcgatgcgg attatcgacc ggctcgaacc gacggcgcgc
     1201 ggcatctacg ccggaacggt cggctatatc gactacgccg aaaatctcga cacctgtatc
     1261 gcgattcgca ccatcgaagt cgaaaacgga tgggcaaacg tgcaggcggg cgcgggcgtc
     1321 gtcgccgatt ccgttcccga aaaggaatac gaggaaacga ttaataaagc gcgcgcgttg
     1381 atgaaagcga ttgaaatggc ggaaaatggt ctttgaaagt gaaaaaggtt aaaagtgaaa
     1441 aggcgaaaaa gtaatcgttg cttttcgtct gcaaactttt tcactttttc accttttcac
     1501 tttttcactt taaaaattat gaaattaaaa atttacttcg ttcttgtttg tttggttttc
     1561 gtcgcggctt gcggcggctc gtcgggcgat acggcgggct cgccggaaat gacgaaaaat
     1621 atgttgaagc tgcgcggctt cgagtttaac gaagacggtt ttttcaaagc tatccggctc
     1681 aacgataccg tagcaatcaa gggatttttc gacgctggaa tggagccgaa cacgaaaaac
     1741 gcgcgcggcg aaacggctct gacttttgcc gtcggcaatg ccgaatcgaa aaccgtaaaa
     1801 gccgtcgccg aaaaagccga catcaatatg caggacaatc tgggacaatc gccgcttcat
     1861 cttgcccttt cgaaacaaaa ggacgaactc tttgattatc tgctggaaaa aaacgccgac
     1921 gtcaatgtcg gcggcgcgaa aggaaatctg agaaatcaaa ccgtgcttta tctagccgtc
     1981 acgcgcgggc gcgaagacct cgtgcagaaa ctgctcgaca aaggcgccga cccgaatatc
     2041 gccgacagcg acggcgcgat tccgctctcg gaagcctgca tcggctcgcg gctcagtgaa
     2101 aacatcgtga aaatgctgct cgacaaaggc gcgaaggtca ataatcagga gaaaaacggc
     2161 gcgactccgc tgatttacat tgcctcgaac aaacaggctt ccgccgaaaa ccgccgcgct
     2221 gtggcgcaga tgctgctcgg cgcgggcgcg gataaaaagc tcagggacaa ggacggcaaa
     2281 accgcgctcg attgggcgaa caaggtcgga aataaagatt tggtcgattt gctgaaataa
     2341 aagttgaccg ggttgaccga gtttggcgag ttgactgagt tgtaaaacgc tcgattaact
     2401 cagtcaactc agtcaactca taactctgaa aactcatgtt attagttatc gataattacg
     2461 attcgtttac ctacaacctc gtgcaatacc tgggcgaact cggcgcgcag atgcaggtgc
     2521 gccgcaacga cgaagtgacg attgaagaaa tcgaaaacga tttaaagccc gaacgcattt
     2581 taatttctcc gggtccggga aacccggacg aagcgggcgt ttctttgaaa gttctggaaa
     2641 gtttttcgga aaagcttcct attctcggcg tctgtctggg gcatcaggca atcgggcagt
     2701 ttttcggcgg aaaggtcgtg cgcgcgcccg tgcccgttca cggaaaatac gtcgaggttt
     2761 gtcacgacgg cgcgggcgtt ttcgcctcgc ttccctatcg ctttaaagcc gcgcgttatc
     2821 attcgctgat tgtcgagcgc gcaggcttgc cgcccgagct ggaagtttcc gccacctcgc
     2881 ccgacggctt gattatgggc ttgcgtcaca gaaaactgaa agtcgaaggc gtgcagtttc
     2941 accccgaatc aatcctgacc agcgaaggca aaaaactttt gcaaaatttt ctgaatcttt
     3001 agctggctga tttgaccgaa gttgaccgaa gttaaacgaa ttgccggaaa catttgactg
     3061 acccggcaaa ctcagccaac ttcagtcagc ttttagtgct ataatcttaa atatgccgaa
     3121 caccgaatgg ctggtcgccg cgccgatgaa cgcgaccggc agcaaagccg atacgccgct
     3181 ccacccgttt ctcgcgcgcc tgatgcgcgg cgagaatttg tcgtttaagg aagccgcgaa
     3241 attcttcgag gctttgacgg acgaagacgc caatcccgcg cagattgcgg gcgcgctggt
     3301 ggcgcttgcc gccaagggcg agacgtttga agaactggcg ggaatggcga aagcgatgcg
     3361 cg
//
LOCUS       DASWNE010000100         5869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_639863, whole genome shotgun sequence.
ACCESSION   DASWNE010000100 DASWNE010000000
VERSION     DASWNE010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5869
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639863"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..826
                     /locus_tag="VGB68_02085"
     CDS             131..826
                     /locus_tag="VGB68_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0048045.1"
                     /translation="MEGKRILVVDDEPQITRVLRRSLAAHRYEVRVAADGESALDTFH
                     DFHPDLIITDLSMPEMSGIELCREIRKTSKIPIIVLSVKGEEKTKVAALDAGADDYVT
                     KPFGMDELLARVRATFRRLSPNEGKESSVLTSGDFSIDLGARKVSVSGKEVHLTPKEF
                     DLLLYLFQNRGRVITHHNLLGAVWGGNFTEQTEYLRVFVGQLRKKIEPQTNQPKYILT
                     VPWVGYRFDTGEE"
     gene            985..1323
                     /locus_tag="VGB68_02090"
     CDS             985..1323
                     /locus_tag="VGB68_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048046.1"
                     /translation="MTNHFVNTANNTLPQNFINRKEFAESYWTIRHIGAGIGLLGGTF
                     ILFGAAFLTIFQFLYSENPHGSWLFALVLPLWIIGAHCFDKVEESDKARRIEYCRQHG
                     MIDEECGKQD"
     gene            1328..2053
                     /locus_tag="VGB68_02095"
     CDS             1328..2053
                     /locus_tag="VGB68_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048047.1"
                     /translation="MRFNKGNIVKFVLIFFISVFCAFVLTADAQQTVFNVPTADVLDK
                     GKVYVEIDAAFKTNDDEALRKFSSFVPRVVAGAGGNVEIGLNLTGNIQPGADTTTLVP
                     TVKWRFYQNKKKDLALFAGNNFYIPVRNRSYKFGAYTYAAVAKTVNKTRLTAGTFLFS
                     KNVVAPNAARGGGQFAIEQTINSKVMLAADWFTGKHANGYFTPGVIYKPHPKVTTYFS
                     YSIGNAYVGRGNHFFLFELGYNF"
     gene            2268..3983
                     /gene="kdpA"
                     /locus_tag="VGB68_02100"
     CDS             2268..3983
                     /gene="kdpA"
                     /locus_tag="VGB68_02100"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEY0048048.1"
                     /translation="MTSSGIFQIALYLVVLTLLAKPTGIYLVRVYNGEKTFLDFALKP
                     IERLVCAATGVDAAREMNWKQYGAATLIFSLVSTLALYVVQRFQGVLPLNPQNFTAAS
                     PELSFNTAISFVSNTNWQSYAGETTLSYFTQMAGLAVQNFASAAVGMALAIAFIRGIA
                     RFETDKLGNFWVDLTRGVLYVLLPISFLAAIFFVSQGVVQNFKPYDAAQTVEGATQII
                     AQGPAASQLAIKMLGTNGGGFFNANSAHPFENPTPFSNFIQMLLILLIPAGFTYTLGR
                     MVKSQGHGWAVYAAMMFLLLAGIFTTYWAESRGNPLFPANIDQTVVGGNFEGKETRFG
                     VANSALFATVTTGASCGAVNSMHDSFTPIGGLVPLVNIMLGEVIFGGVGAGMYGILVF
                     VILTVFIAGLMVGRTPEYLGKKIEAYDVQMAMLYALISPLVILALSAISAVSPNFGLT
                     SLNNQGAHGLSEILYAFTSATGNNGSAFAGLSANTLWYNTTLGMAMLVGRFLMIVPML
                     AIAGNLARKKAVPPSLGTFPVNTTLFSVLLISVIIILGALTFFPALSLSPILEHFQMI
                     NGQTF"
     gene            3991..>5869
                     /gene="kdpB"
                     /locus_tag="VGB68_02105"
     CDS             3991..>5869
                     /gene="kdpB"
                     /locus_tag="VGB68_02105"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HEY0048049.1"
                     /translation="MKKTISIWDKNIIAQALIDSIKKLNPGRMMKNPVMFVVEGGAAF
                     LTLKLLVGIFAPSAESNFGFELQITLWLWFTVLFANFAEAMAEGRGKAQADTLRKAKT
                     ETSAKLLKTDGTTEIVGATELRKDNVVLVEAGEFIPGDGEIIEGVASVDESAITGESA
                     PVIRESGGDRSAVTGGTRVLSDWIKVRISSNPGETFIDRMIALVEGASRQKTPNEIAL
                     NILLAGLTIIFLLAVVTLQPFAIYSNAAQTVFVLISLLVCLIPTTIGGLLSAIGIAGM
                     DRLVQHNVLAMSGRAVEAAGDVNTLLLDKTGTITLGNRQATKFIPLQGVDEESLADAA
                     QLSSLADETPEGRSIVILAKEKYGLRGREIHELAENAEFIPFTAQTRMSGVNLDGREI
                     RKGAVDAIEKYVSGNGFQSAELRVVVEWVAKQGGTPLVVADDRKPLGVIYLKDIVKGG
                     MSERFNQLRRMGIKTVMITGDNPLTAASIAEEAGVDDFLAEATPEDKMALIKREQSEG
                     KLVAMTGDGTNDAPALAQADVGVAMNTGTQAAKEAGNMVDLDSDPTKLIEVVEIGKQL
                     LMTRGALTTFSIANDVAKYFAIIPAMFAATFPVLNALNIMNLKTPESAILSAVIFNAL
                     VI"
BASE COUNT         1374 a         1453 c         1603 g         1439 t
ORIGIN      
        1 tcgcagggcg gaagtatccg gattgaagac gggcgcgacg aatacgcgac gcgcgcggtt
       61 tttaccgtgc cgattggcga tattgaaaaa taattactaa gcttaaccaa agcgcgcata
      121 aaataaagca atggaaggaa aaagaatttt agttgtcgac gacgaaccgc agatcacgcg
      181 cgttctgcgg cgcagcttag ccgcgcaccg gtacgaagtc cgggttgccg ccgacggcga
      241 atcggctctc gacacattcc acgattttca tccagattta attatcaccg acctttcgat
      301 gcctgagatg agcggcatcg agctttgccg cgaaatccgc aaaacatcaa aaatcccgat
      361 tatcgtgctt tccgtcaaag gcgaggaaaa aacaaaggtc gccgcgctgg acgcgggcgc
      421 ggacgattac gtgaccaaac ccttcgggat ggacgagctg ctcgctcgcg tgcgcgcgac
      481 ctttcgccgc ttgtcgccaa acgagggaaa ggaatcttcc gttttaacct ccggcgactt
      541 ttctatcgac ctcggcgcgc gcaaggtttc cgtttcggga aaagaagttc acctgacgcc
      601 gaaggaattc gatttgctgc tttatctttt tcaaaatcgc ggtcgcgtca ttacgcatca
      661 caatcttctg ggagcagtct ggggcggaaa ttttaccgag caaaccgaat atctaagggt
      721 tttcgtcggg cagcttcgca aaaaaatcga gccgcagacc aatcagccga aatatatttt
      781 aaccgttccg tgggtcggct accgctttga tacgggcgaa gaataaggat tacaacaaaa
      841 ctgctgattt cctaaattcc ggctctcaag cctacagcct gttcttcctt ctgcgtctaa
      901 aaaatttatg aacattttat aaatccttta ctccatcctt atacctctag tcatattctg
      961 tttccggtga gcgagaaaaa gaatatgaca aaccatttcg taaacacggc aaacaatact
     1021 ttgccgcaaa acttcattaa ccgaaaagaa tttgccgaaa gctattggac gatccggcac
     1081 atcggcgcgg ggatcggtct gctcggcgga acctttattc ttttcggcgc ggcttttctg
     1141 acaatctttc aatttctata cagtgaaaac ccgcacggca gctggctgtt cgcgctcgtt
     1201 ctgccgttat ggattatcgg agctcattgt ttcgacaaag ttgaggaatc cgataaagcc
     1261 cggagaatcg agtattgcag acaacacggg atgattgacg aggaatgcgg caaacaggat
     1321 tagtcggatg agatttaata aaggaaatat cgtgaaattc gttcttatat tttttatctc
     1381 tgttttctgt gcgtttgttt tgaccgccga tgcgcagcag acggtcttta acgttccgac
     1441 ggcggacgtg ctggataaag gcaaagttta cgtcgaaatc gacgctgcgt ttaagacgaa
     1501 cgacgatgag gctttgcgaa agttttcgtc attcgtgccg cgcgttgtcg caggagcagg
     1561 cggaaacgtc gaaatcggtc tgaatttgac gggaaacatt cagccgggcg cggatacgac
     1621 aacgctcgtg ccgacggtga aatggcgttt ttatcaaaac aaaaagaaag atttagcgct
     1681 tttcgcggga aataattttt atattccggt gcgaaatcga agctataagt tcggagctta
     1741 tacttacgcg gcagtcgcca aaacggtcaa caaaacccgc ttgaccgccg gaacattttt
     1801 attttcaaaa aatgtcgtcg cgccgaatgc ggctcgcggc ggcggtcaat ttgccatcga
     1861 gcaaactatt aattcaaaag taatgctcgc cgccgactgg ttcaccggca aacacgccaa
     1921 cggctatttt acgcccggcg tgatttacaa gccgcacccg aaagtgacga cttatttcag
     1981 ctattcaatc ggaaatgcat acgtcggcag gggaaatcat tttttcctgt tcgagctcgg
     2041 ctacaacttt taattcggag attccaggaa atgtcagacg taatttttat cgccgtcatc
     2101 gccggatttt ttctactggc tttggcttat ctgaaaggct gcgaagctct gcggaaaggg
     2161 gcgttgaaca aatgagctgg gatattgttt tggcgacgat ctgcgcggct ttattgctga
     2221 tttatttaac gtatgcgctt ttgcgcccgg aaaaatttta agtttatatg acatccagcg
     2281 gaattttcca aatcgcactt tatctcgtcg tgctgacgct tctggcaaaa ccgacgggca
     2341 tttatttggt cagagtttat aacggcgaga agacgttttt ggattttgct ttaaagccga
     2401 tcgagcgatt ggtttgcgcg gcgaccggag ttgatgcggc gcgcgagatg aactggaagc
     2461 aatacggcgc ggcaacgctc attttcagtc ttgtttcgac gctcgcgctt tatgtcgtcc
     2521 agcgttttca aggcgttctg ccgctcaatc cgcagaattt taccgcagct tcgccggagc
     2581 tttcgttcaa tacggcaatt tcatttgtct cgaacacgaa ctggcagtct tacgccggcg
     2641 agacgacgct gagctatttt acgcagatgg ctggtttggc ggtccaaaat tttgcttcgg
     2701 cagcggtcgg aatggcgctg gcaatcgctt ttatccgcgg aatcgcgaga tttgaaaccg
     2761 ataagctcgg caatttctgg gtcgatttaa cgcgcggagt gctttacgtt ctgctgccga
     2821 tttcgtttct agccgccatc tttttcgtct cgcagggcgt ggtgcagaac ttcaaaccgt
     2881 atgacgcggc gcagaccgtc gaaggcgcga cgcagataat cgcgcagggt ccggcggctt
     2941 cgcagctggc gataaaaatg ctcgggacaa acggcggcgg attttttaac gccaactcgg
     3001 cgcatccgtt cgaaaatccg acgccgtttt caaactttat ccagatgctc cttattctgc
     3061 tgattccggc gggttttacc tacacgctcg gtcggatggt gaaatcgcag ggacacggct
     3121 gggcggttta tgcggcgatg atgtttttgc tgctggcggg aatcttcacg acctattggg
     3181 cagaatcccg cggcaatccg ctttttccgg caaacatcga tcaaacggtg gtcggcggaa
     3241 attttgaagg caaggaaacg cggttcggcg tggcgaattc cgcactgttt gcaactgtca
     3301 cgacgggcgc atcgtgcggc gcggtcaatt cgatgcacga ttcgtttacg ccgattggcg
     3361 ggcttgtgcc gcttgtcaat attatgctcg gcgaagtcat cttcggcggc gtcggagcgg
     3421 gaatgtacgg cattttggtg tttgtcatcc tgacggtttt tatcgccggt ttgatggtcg
     3481 gcagaacgcc cgaatatctc ggcaaaaaga tcgaagccta cgacgtgcag atggcgatgc
     3541 tttacgcgct gatttccccg ctcgtgattc tcgctttgag cgcgatttcg gcggtctctc
     3601 cgaatttcgg tctgacttcg ctcaataatc aaggcgcgca cgggctttcg gaaattcttt
     3661 acgcctttac gtccgcgacg ggcaataacg gctcggcttt tgccggctta agcgccaata
     3721 cgctctggta taacacgacg ctcgggatgg cgatgctcgt cggcagattt ctgatgatcg
     3781 tgccgatgct ggcgattgcg ggaaatctgg cgcgaaagaa agccgtgccg ccgagtctcg
     3841 gcactttccc ggtcaacacg acgcttttca gcgttttgct catcagcgtg atcattatcc
     3901 tgggcgcgct gacgtttttc ccggcgctga gcttgtcgcc gattctggag catttccaga
     3961 tgattaacgg gcagactttt taatacgaaa atgaaaaaga cgatttcaat ttgggataaa
     4021 aacattatcg cgcaggcttt gatcgattcg atcaaaaagc tcaatccggg caggatgatg
     4081 aaaaacccgg tgatgttcgt ggtcgagggc ggcgcggcgt ttttaacctt aaaactttta
     4141 gtcggtattt tcgcgccgtc ggctgagtcg aattttggtt ttgaattgca aatcacgctc
     4201 tggctttggt tcaccgtttt attcgccaat ttcgccgagg cgatggcgga agggcgcggc
     4261 aaggcgcaag ccgatacatt gcgaaaagca aaaaccgaaa cgagcgcaaa gcttttgaaa
     4321 acggacggga cgacggaaat tgtcggcgcg accgagctta gaaaagataa cgtcgtgctg
     4381 gtcgaagccg gcgaatttat tcccggcgac ggcgaaataa tcgaaggcgt cgcgtcggtc
     4441 gacgaatcgg cgattacggg cgagagcgct ccggtcatcc gcgagtcggg cggcgaccgc
     4501 tcggcggtga cgggcggaac gcgcgttttg tcggattgga taaaagtgcg gatttcatca
     4561 aatccgggcg agaccttcat tgaccggatg atcgcgctcg tcgagggcgc gtcacgccag
     4621 aaaactccga acgaaattgc tttgaatatc ctgctcgccg gtttaacgat tatttttctg
     4681 ctcgcggtcg tgacgttgca gccctttgcg atttattcaa acgccgcgca aaccgttttc
     4741 gtgctgattt cgctgctcgt ctgcctgatt ccgacgacta tcggcggact gctttcagcc
     4801 atcggcatcg ccgggatgga caggctcgtc cagcacaatg ttctggcgat gagcgggcgc
     4861 gccgtcgaag cggcgggcga cgtcaatacg cttcttctgg ataaaaccgg aacgattaca
     4921 ctaggcaacc gtcaggcaac aaaatttatt ccgcttcaag gcgtcgacga ggagagttta
     4981 gccgatgcgg cacagctttc ttctttagcg gacgaaacgc ccgaaggtcg ttcgatcgtc
     5041 attctggcga aagaaaaata cggtttgcgc gggcgcgaga ttcacgaatt ggcagagaac
     5101 gctgaattta ttccgtttac ggcgcaaacg agaatgtcgg gcgtcaatct cgacgggcgc
     5161 gaaatccgta aaggcgcggt cgatgcgatt gagaaatatg tttccggcaa cggatttcaa
     5221 tcagccgagc tgcgcgtcgt agttgaatgg gtcgccaaac aaggcggcac gccgcttgtc
     5281 gtcgccgacg atcgcaaacc gctgggcgtc atctatctga aagacatcgt caagggcggt
     5341 atgagcgagc gttttaacca gctgcgccgg atgggaatca aaaccgtgat gattacgggc
     5401 gataatcctc tgaccgcggc ttcgatcgcc gaggaagccg gcgttgacga ttttctcgcc
     5461 gaagccacgc ccgaagacaa aatggcttta atcaagcgcg aacagtcgga aggcaaatta
     5521 gtggcgatga ccggcgacgg cacgaacgac gcgcccgcgc tcgcgcaggc ggacgtcggc
     5581 gtggcgatga acacgggaac gcaagccgct aaagaagccg gaaatatggt cgatttggac
     5641 agcgacccga caaagctcat cgaggtggtc gaaatcggca aacagctttt gatgacgcgc
     5701 ggcgctctga cgacattctc aatcgccaac gacgtggcaa agtatttcgc cattattccg
     5761 gcgatgttcg cggcgacgtt tccggttttg aacgctttga atattatgaa tttgaaaacg
     5821 cccgaatcgg cgattctttc cgccgtaatt tttaacgcgc tggtcatcg
//
LOCUS       DASWNE010000101         5123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_463025, whole genome shotgun sequence.
ACCESSION   DASWNE010000101 DASWNE010000000
VERSION     DASWNE010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463025"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VGB68_02110"
     CDS             <1..423
                     /locus_tag="VGB68_02110"
                     /inference="COORDINATES: protein motif:HMM:NF021915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048050.1"
                     /translation="HAESLEKLEPEDLIRYGLIPEFVGRLAVLGTLHELDEKALIQIL
                     TEPKNALIKQYQRKFEFDNIRLKFTDDAIKAIAKQAYERKVGARGLMMIMEEILLESM
                     YHLPSQKKVKELVITSDMVERKAPVFEVIGEVDTEAAA"
     gene            complement(511..1095)
                     /locus_tag="VGB68_02115"
     CDS             complement(511..1095)
                     /locus_tag="VGB68_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048051.1"
                     /translation="MSKINRKFFFEHVRNSLFGKSLTQKQVDGMTAILDEWEAKHTKK
                     DDRWLAYMMATVYHETDQKFQGIEEYGPDSYFKKYDGRKDLGNTEPGDGLRFKGRGFV
                     QITGRLNYTKFSKILGIDLVDNPKLALDLENCVKILFYGMTNGTFTGRKLSDYFSATA
                     EEWVNARRIINRLDKANLIADEAKKFYAAISYTA"
     gene            1383..3266
                     /locus_tag="VGB68_02120"
     CDS             1383..3266
                     /locus_tag="VGB68_02120"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY0048052.1"
                     /translation="MEPILIFSTGAAETQTFPLKGIESVTLGRAETNEIVLSDGDVSR
                     CHCSVKEANGSYYLMDLDSRNGTFLNNAPVREAVLRHGDCIRIGKTLIWFQTDKELNA
                     IMPFNDNFQFDNGDLITRSEIRFAVEPNLHKMPQDLNALARLGRALNESRNTAHLQSR
                     FLEIILELIPAAERGVILLVEDNLNEPKSVSTLTRNLLNTEKITVSRTVTERVLNEKI
                     ALLSNDLRDSGLENAESLIAKRVAALLCVPLQLNEDRGLIYLDSCQPDAKFTQNHLQQ
                     LTAISVLISAALEQRLSIQALERENARLQTELEIETNLIGVSQEINKVFQLIKKVSPT
                     DSNVLITGESGTGKELVAQTLHRNSRRTDKPFVAINCAVLNEHLLESDLFGHEKGSFT
                     GAIAQKKGKLEIADGGTVFLDEVGELASHLQAKLLRVLQEREFQRVGGNITLKTNVRI
                     IAATNQNLQENVRKGTFREDLFFRLNVVQINVPPLRERKTDIPLLAQHFVNKYSERCN
                     RKVVGLSKKAREILSNYEWTGNVRELENVIERAIVLGSTETIMPEDLPDEIIENAVFA
                     EESSENGFHMQLKEAKRRIIVAALFEAKGHYTEAANSLGIHPNNLHRLVKSLGIKEEV
                     KKA"
     gene            complement(3270..>5123)
                     /locus_tag="VGB68_02125"
     CDS             complement(3270..>5123)
                     /locus_tag="VGB68_02125"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HEY0048053.1"
                     /translation="FNGIGSQFFSPQTPPPDKNKSVAIKNYSELISRKPRKGFLILFA
                     AIGFLIVGIIGLFYLFSTVSPEPPTQIKITRLSTSGNTTNAAISPDGRVIAYVENENG
                     QYSLWVRQTEETAGNQIIAPGSSSFSNLTFSRDGNWIYYTKFEVSSTGTLYRTPYMRG
                     SQQEIAKDVDSAISFSPDGSEFAFIRSTPDEGTDSIIISKLDGTNERVLTQKKRPEFF
                     IKAVRESLAWSPDGKMIACPFGKTSNEGDLMSIVEIDVASGEEKRLTAKIWSRVGRVV
                     WTNDLNKLLITAADFGSDLYQIVQLTRPDGKTKNITSEISDYFSISLNKDSNRLLATI
                     YDKNSRISTSFLTQINQLKRLAGGGYDGIGGVNWTTDNRIVFVSIESGNPDIWIMNAN
                     GTNRRQLTFEKSSDEYPVVSKDGQNIVFVSSRTGTPHIWRMNFDGGDLRQLTYKGGES
                     FPQITPDGKFVIFSSQNDRRRTLWKVPIEGGEPMQLTEKLSHWSAVSPDGKMIACLTR
                     GDNHTDPMMLAVISAETGKFLKIFDFSGDASPSLTPTIRWTPDGKALAYVDTVDGISN
                     LYLQQLSGGAPKKLTDFSADRIFSFDFSSDGKSVVLARGVVRNNLVLIENF"
BASE COUNT         1312 a         1268 c         1258 g         1285 t
ORIGIN      
        1 cacgccgaat ctctggaaaa gctcgaaccc gaagatttga ttcgctacgg cttgattccg
       61 gaattcgtcg ggcgtctcgc cgttctggga acgctgcacg aattggacga aaaagccctg
      121 attcaaattt tgaccgagcc gaaaaacgcg cttatcaagc agtatcagcg caagttcgag
      181 tttgacaaca ttcgtctgaa atttaccgac gacgccatca aagccatcgc caagcaggct
      241 tatgaacgca aagtcggcgc gcgcggtctg atgatgatta tggaagaaat tctgctcgaa
      301 tcgatgtatc atctgccgtc gcagaaaaag gtcaaggaac tcgtcatcac cagcgatatg
      361 gtcgagcgca aagcgccggt ttttgaagtt atcggcgaag tcgacacgga agccgccgcc
      421 tgaaaaaggg taaaggataa aggcgaaaga tagaggataa aggcgaaaga tagaggaatt
      481 atattccttt tcctttcgcc tttacccttt tcaagccgtg tagctaatcg cggcgtagaa
      541 ttttttcgct tcgtcggcaa tcagattcgc tttatccagg cggttaataa tccggcgcgc
      601 gttgacccat tcttccgccg tcgccgagaa ataatcggat aacttcctgc ccgtaaacgt
      661 gccgtttgtc ataccgtaga acaagatttt cacgcagttt tccaaatcca gcgcgagctt
      721 tggattatcg accaaatcga tgccgagaat tttgctgaat ttcgtataat tgaggcgtcc
      781 cgtaatctgc acgaaacctc tgcctttgaa tctcagcccg tcgcccggct cggtattgcc
      841 taaatccttg cgaccgtcgt attttttgaa atacgaatcg ggtccgtatt cctcgatgcc
      901 ctgaaacttc tggtcggtct cgtgataaac ggtcgccatc atatatgcca gccagcggtc
      961 gtctttcttt gtatgtttgg cttcccattc atcgagaatc gccgtcatac cgtcaacctg
     1021 cttttgcgtc agacttttgc cgaacaatga atttctgacg tgttcaaaaa agaatttgcg
     1081 atttatttta ctcatagatt ttatccttca atcttatttt tcaaattaat ttaaaactac
     1141 cgcaatttat cattatcaaa accgaataac caaatccgct tttaatcaaa acggcaatgc
     1201 agaattcatt ccgcaaaaat ttattgcctg acgaatcttt atttataagc ttttttttta
     1261 ccgtaaataa gctctttgtt tcgagttata tcaattctga tagtttggct atcagaattg
     1321 atataggtta tgatgcttga cgagttccga ttctaattca ttctcctgaa ggaaattaaa
     1381 aaatggagcc catactgatt ttttctaccg gcgcagcaga aacccaaaca ttcccgctga
     1441 agggaatcga atcggtcacg ctcgggcgcg ctgaaaccaa cgaaattgtc ctttccgacg
     1501 gcgacgtctc gcgctgtcac tgctccgtaa aggaagccaa cggcagttat tatctgatgg
     1561 atttggacag ccgcaacggg acgtttctga acaacgcgcc cgtcagggaa gctgtcttaa
     1621 ggcacggtga ttgcatccgc atcggcaaaa ctcttatctg gtttcagacc gataaagagc
     1681 tgaatgcaat aatgcctttc aatgataatt tccaatttga taacggcgac ttgattacgc
     1741 gttccgagat tcgttttgcg gtcgagccta atcttcataa aatgccgcag gatttgaacg
     1801 cgctggcgcg gctcgggcgg gctttaaatg aaagccggaa cacggcgcac ctgcaaagcc
     1861 gttttctgga gattattctc gaattgattc cggcggcgga acgcggcgta attctgctgg
     1921 tcgaagacaa tcttaacgag ccgaaatccg tctccacgct gaccagaaac ctgctcaaca
     1981 cggaaaaaat aaccgtcagc cgcacggtga ccgagcgcgt tttaaacgaa aaaatcgctc
     2041 tgctcagcaa tgacttaaga gatagcgggc tggaaaacgc cgaaagctta atcgccaagc
     2101 gcgtcgcggc tctgctctgc gtcccgctcc agcttaatga agatcggggc ttgatttatc
     2161 tggattcctg ccagccggac gcgaaattta cgcaaaacca tttgcagcag ctaacggcga
     2221 tttccgtttt aatttcggcg gctttggagc agagactttc catacaggcg ctggaaagag
     2281 aaaacgcccg gctccaaacc gagctggaaa tcgaaaccaa tctgatcggc gtcagccagg
     2341 aaataaacaa ggtttttcaa ctgattaaaa aagtttcgcc gaccgattcc aacgttttga
     2401 taacgggcga atcgggaacg ggaaaagagc tcgtggcgca gacgctgcat cgcaacagcc
     2461 gccgcacgga taagcctttt gtggcgataa actgcgccgt tttaaatgag catctgctgg
     2521 aaagcgacct tttcggacac gagaaaggct cttttaccgg agccatcgcg cagaaaaaag
     2581 gaaagctcga aatagcggac ggcggaacgg tttttctgga cgaagtcggc gagcttgcct
     2641 cgcatctgca ggcaaagctt ctgcgcgttt tgcaggagcg cgagtttcag cgcgtcggcg
     2701 gaaacattac gctgaaaacg aatgtccgga taatcgccgc gaccaatcag aatttacagg
     2761 aaaacgtccg taagggaact tttcgcgaag accttttctt tcgcttaaac gtcgtgcaga
     2821 taaacgtgcc gcccctgcgc gagcgcaaaa cggatattcc gctgctggcg cagcatttcg
     2881 tcaacaaata cagcgagcga tgcaaccgaa aagtcgtcgg tctgtcgaaa aaagcacgcg
     2941 aaattttgag caattatgaa tggacgggca acgttcgtga gctggaaaac gtgattgagc
     3001 gcgccattgt tctcggctcg accgaaacca ttatgcccga agacctgccg gatgaaatta
     3061 tcgaaaacgc ggtttttgcc gaggaatctt cggagaacgg ctttcatatg cagcttaaag
     3121 aagccaagcg aagaatcatc gtggcggctc tgttcgaagc caaagggcat tatacggaag
     3181 cggcaaactc gctcggcatt catccgaaca atctgcatcg tttagtaaaa agcctcggaa
     3241 tcaaggaaga agtcaaaaaa gcttaagttt taaaaatttt caatcagcac taaattattt
     3301 cgcactacgc cgcgcgccag aacgactgat tttccgtcgc tcgaaaaatc gaacgaaaaa
     3361 atccggtcag ccgaaaaatc cgtcagcttt ttcggcgcgc cgcccgatag ttgttgcagg
     3421 taaaggtttg agatgccgtc gacggtatcg acgtaggcga gcgcttttcc gtccggcgtc
     3481 cagcgaatcg tcggcgtcag gctcggcgaa gcatcgccgg aaaaatcgaa aattttcaaa
     3541 aattttcccg tttccgccga aatgacggct aacatcatcg ggtcggtgtg attatcgccg
     3601 cgcgtcaggc aggctatcat tttgccgtcg ggcgagaccg ccgaccagtg agacagtttt
     3661 tcggttagct gcatgggctc gccgccttcg ataggaactt tccagagcgt ccggcgccgg
     3721 tcgttttgcg atgaaaagat aacaaatttg ccgtcgggcg taatttgcgg aaaagactcg
     3781 ccgcctttgt aggtcagttg tctgaggtcg ccgccgtcga aattcattcg ccagatgtgc
     3841 ggcgttcccg tccgcgaaga aacaaaaacg atgttttgcc cgtctttgga aaccaccgga
     3901 tattcgtcgc tggatttttc aaacgtcaat tgccgccggt tcgtgccgtt ggcgttcatt
     3961 atccagatgt cgggatttcc gctttcaatc gaaacaaaga cgatgcggtt gtccgtcgtc
     4021 cagttgacgc cgccgatgcc gtcatagccg ccgccggcga gtcgtttgag ctggttgatt
     4081 tgggttaaaa aagatgtcga aattctcgaa tttttatcgt agatagtcgc cagcaggcga
     4141 ttcgaatctt tattcagact gatgctgaaa taatcgctga tttcgctggt gatatttttt
     4201 gtttttccgt ccggacgcgt cagctgcacg atttgataca agtccgaacc gaaatcggct
     4261 gccgtaatca aaagcttgtt caaatcattc gtccagacaa ctctgccgac gcgcgaccag
     4321 attttagcgg tcagccgttt ttcctctccg ctggcaacat cgatttcgac gatgctcatc
     4381 aaatcgcctt cattcgaggt ctttccgaaa ggacaggcga tcatttttcc atccggcgac
     4441 cacgccaggc tttcacggac ggcttttata aaaaattccg gtcttttctt ttgtgtcagg
     4501 acgcgttcgt tagtgccgtc gagtttggaa atgattatgc tgtcggtgcc ttcgtccggc
     4561 gtcgagcgga taaaagcgaa ctcgctgccg tcgggcgaaa aagaaatcgc cgaatcgaca
     4621 tctttcgcaa tttcctgctg tgagccgcgc atatagggcg tccggtaaag cgtcccggtt
     4681 gagctgacct caaatttcgt gtagtaaatc caatttccgt cgcgcgaaaa cgtcaaatta
     4741 ctaaatgagg aagagccggg cgcgataatc tggtttccgg cggtctcttc ggtttgtctg
     4801 acccagaggc tatactgccc gttctcattt tcaacgtaag caatcacccg cccgtccggt
     4861 gaaattgccg cgttggtcgt gttgccgctg gtcgataggc gtgtgatttt tatctgcgtc
     4921 ggcggctccg gcgaaacggt cgagaaaaga taaaacaacc cgatgatgcc gacaatcaaa
     4981 aaaccgattg cggcaaacag aatcagaaag cctttccggg gcttgcggga aatcaactcc
     5041 gaataattct taatcgctac cgatttgttt ttatccggcg gcggcgtttg gggcgaaaaa
     5101 aactggctgc ctattccgtt aaa
//
LOCUS       DASWNE010000102         4636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_427401, whole genome shotgun sequence.
ACCESSION   DASWNE010000102 DASWNE010000000
VERSION     DASWNE010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427401"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1650)
                     /locus_tag="VGB68_02130"
     CDS             complement(<1..1650)
                     /locus_tag="VGB68_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048054.1"
                     /translation="MNQQKSAVSFYYPRLFILGLAGLLAVLSTALWLRSAPVSAQAED
                     ELSPAIVVFSESFDNVTAPALPAGWTTASSGQIDSFKTVTNFPDTLPNAAFVHDPNTQ
                     GIAELVSPAIALGNIQHKLIFRHFYQTDYEFDGGVLEISVNGGAFQDIVTAGGTFVSG
                     GYDTPLVGSSLAGRRAWTGQQSGYVTTEVTLPSGTANQSIRLRWRIATDPMEAGTGWW
                     IDSIQITNAISGVNSGAISIPSVGAASPYPSEINVTNHEGFVTGVQVTLTNFSHNSPD
                     DVDLLLVSPSGRKVVLMSDVGGNNPAINLNLFFSDAATASLPDNSALVSGTYKPTNFE
                     PNDAFSAPAPGGAPTGTRLSALNGSTANGAWQLFLVDDSGNNAGSVSGGWNIFVQSSP
                     DAISIPDVGTAQPYASEKQISGNQGTITKATVTLSNFSHTAPDDADLLLVAPNGRKVV
                     LMSDVGGNTEVGGLNLIFDDAAAGNLPDNAPLVSGTFKPTDFEPGDAFPAPAPSGATG
                     TTLDAFYGSAPNGTWKLFVVDDAGENSGSIGGDWSVNLQTST"
     gene            1993..4227
                     /locus_tag="VGB68_02135"
     CDS             1993..4227
                     /locus_tag="VGB68_02135"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HEY0048055.1"
                     /translation="MFIISDACRRRRLSALSVFCCLLLFGSVFGQTNTLPEDAAPVLI
                     SEPNSTRALITVANGKRGASLPTGIVQPGRQTIVTFFVTNLDLLEGEGANAFRAEFED
                     SSHYRYPLEIVRFERTAERKQVYALSVRLGAEIGSVGDVLARVTWRGMSSNRVRLSIG
                     YEGGKIADDAGAVPTPMPGTQAAARKLSANRAALPWTGDRVRFMEQTSFGVNAALELR
                     LRRLGYSTWLEEQMDEASYSAYTYPNYALQPTTPSATCDGNTQPADVPVTCFRDNYTM
                     YLLQNWFYKEALYNENQQLRRRVSWALSQVLVTSGRNLVQPSRMMPYVQTLDRNAFGN
                     FRTLLEEITLNPAMGQYLDMAISTRTSPNENYAREILQLFSIGVDLLNQDGTPVLDNQ
                     GNRIPSYNQEIINNFTKVFTGWSYCQNPANCPNWQPGLVNYRDPMFAVAANHDTGAKT
                     LLNYAGATPNIPAGQTPEEDMDAALNNIFYHPNVAPFISKLLIQQLVTSNPTPAYVGR
                     ISAVFNDNGTGTRGDLKAVIRAILLDPEARGNFKTDPDYGKLREPVLYITSVLRPFNP
                     TANNNLAVPASCNGQSDGVINGITQPLEQDVFNAPNVFNYYSMDYIIPGTPLAGPEFG
                     IFSTGTALKRPNFVNQMAPPGSASTTAGILAVTGSAASPTSIPCGTRIDLSRLQALAA
                     SDPTGAALVDTLNRELMHGSMSAAVRSDILTAVQAVAAANTLKRARTAFYLVTTLTQY
                     QVQR"
     gene            4312..>4636
                     /locus_tag="VGB68_02140"
     CDS             4312..>4636
                     /locus_tag="VGB68_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048056.1"
                     /translation="MKENRRDFLKKSCRVLSMTAIATQMRHFGLVNVMAQERADTQKS
                     AGGDDYKALVCVFLDGGNDGNNSVIPSYDEGYNQYAAARGGTFGLAIPRNSLLPITPP
                     NMGGQV"
BASE COUNT         1044 a         1313 c         1296 g          983 t
ORIGIN      
        1 ggtcgaagtt tgcagattga cgctccagtc gccgccgatg cttcccgaat tttcgcccgc
       61 gtcgtcgacg acaaacaatt tccaggttcc gttcggcgca ctgccgtaaa aagcatcgag
      121 cgtcgtgccc gtcgcgcctg acggcgcggg agcgggaaaa gcgtcgcccg gttcaaaatc
      181 ggtcggcttg aaagttcccg acacgagcgg cgcgttgtcc ggcagatttc cggcggcggc
      241 atcgtcgaaa attaaattaa gcccgccgac ttccgtattt ccgccgacgt ccgacatcaa
      301 gacgactttc cgcccgttcg gcgcgaccaa tagcaaatcg gcatcgtccg gcgcggtgtg
      361 gctgaaattc gagagcgtga cggtcgcttt ggtaatcgtt ccctggttgc cggaaatttg
      421 tttttccgac gcgtaaggct gcgccgtgcc gacgtcggga atggaaatcg cgtcgggcga
      481 gctttgcacg aaaatgttcc agccgccgga gacgctgccc gcgttgttgc cgctgtcatc
      541 gaccagaaac aactgccacg cgccgttcgc cgtcgagccg ttgagcgccg aaagtctcgt
      601 gccggtcggc gcgccgccgg gtgcgggcgc ggaaaatgcg tcgttcggct cgaaattcgt
      661 cggtttgtag gtgccgctga cgagagccga attgtcggga agcgaagcgg tcgcggcatc
      721 gctgaaaaac aaattcagat taatcgccgg attgtttccg ccgacgtccg acatcaagac
      781 gactttccgt ccgctcggcg agaccagcag caaatcgacg tcgtccggcg aattgtgact
      841 gaaattcgtc aaagtaacct gcacgcccgt cacaaatccc tcgtgattcg tgacgtttat
      901 ttccgaagga tacggcgaag ccgcgccgac gctcggaatc gaaatcgcgc cggaattgac
      961 gccggagatg gcgttcgtta tttgaatgct gtcaatccac cagccggttc cggcttccat
     1021 cgggtcggtc gctatccgcc agcgcagccg tatgctttga ttggcggtgc cggacggaag
     1081 agttacttcc gtcgtgacat agccggattg ctgtccggtc caggcgcgcc tgcccgctaa
     1141 agagctgccg acgagcggcg tatcgtaacc gccgctgacg aaagtgccgc cagccgtcac
     1201 aatatcctga aatgctccgc cgttgacgct gatttccaga acgccgccgt caaattcata
     1261 atcggtttga taaaaatgcc ggaaaattaa tttatgttga atattgccga gtgcaatcgc
     1321 gggcgagacg agctcggcga tgccctgcgt gttcgggtcg tggacgaaag ccgcgttcgg
     1381 caacgtatcg ggaaaattcg tcaccgtttt aaacgagtcg atttgcccgg aactggcggt
     1441 cgtccacccg gcgggcagcg ccggcgcggt cacgttgtcg aagctttcgc tgaagacgac
     1501 tatcgccggg ctcagttcat cttccgcctg cgcgctgacc ggcgccgagc gaagccagag
     1561 cgccgtcgaa agaaccgcca gcaaaccggc aagacctaaa ataaataatc gcggataata
     1621 aaaggagacg gcagattttt gttgattcat taaacttttc tcaccgaaat aaaatcaggc
     1681 gcgggggcag gtgccgactt tacgaaatac tcaagcgtag actcaaaacc ttatcaattc
     1741 aggctgcgta ttaatgaccc gacagaccaa aagattgcag gcgttggcga agaattatca
     1801 acttttttga gagaattagc aagaacattt caatggtaaa gtttttttta atcgcctcgc
     1861 gtctcaaaaa aattgccttt tctccgcgaa agagttataa atcaatgtta tctttttatc
     1921 gcgttctatg atttcctgcc caactgaatc ggatttcttg tttcgggtga aaagctcggg
     1981 agaaaaaaaa taatgtttat aatatctgat gcctgtcgcc gccgacggct ttcggcgctc
     2041 agcgtttttt gttgtctttt attgttcggc agcgtcttcg gtcaaacaaa caccttgccc
     2101 gaagacgccg cgccggtttt gatcagcgag ccgaattcga ccagagcttt gattaccgtg
     2161 gctaacggga aacgcggcgc gagcctgccg accggaatcg tccagcccgg caggcagaca
     2221 atcgtcacat ttttcgtcac caatctggat ttgctcgaag gggaaggcgc aaatgctttt
     2281 cgcgccgagt ttgaagattc aagccattat cgctacccgc tggaaatcgt tcgctttgag
     2341 cggacggcgg agcgcaaaca ggtttacgct ctgagcgttc gcctcggcgc ggaaatcggc
     2401 agcgtcggcg acgttctggc gcgcgtcacc tggcgcggaa tgtcgagcaa tcgcgtgcgg
     2461 ctttcaatcg gctacgaagg cgggaaaatt gcggacgacg caggcgcggt tccgacgccg
     2521 atgccgggaa cgcaggcggc agcccgcaaa ttgtccgcca atcgcgccgc gctgccctgg
     2581 acgggcgacc gcgtgcgctt tatggaacag acgagtttcg gcgtcaacgc ggcgctcgaa
     2641 ctgcgcctgc gccgcctcgg ctacagcacg tggctggaag agcagatgga cgaagcgagc
     2701 tattcggcgt atacgtatcc gaattatgcg cttcaaccga cgacgccctc ggcgacctgc
     2761 gacggcaaca cacagccggc ggatgttccg gtaacctgtt tccgtgacaa ttacacgatg
     2821 tatctgctcc agaactggtt ttataaagaa gcgctttaca atgaaaatca gcagttgcgg
     2881 cggcgcgtct cctgggcttt aagtcaggtt ctggtcactt cgggacgcaa cctggtgcag
     2941 ccgagccgga tgatgcctta tgtccagacg ctcgaccgca acgccttcgg caatttccgc
     3001 acgctgctcg aagaaatcac tctgaatccg gcgatggggc aatatctgga tatggcaatc
     3061 agcacgcgca ccagcccgaa cgaaaattac gcgcgcgaga ttttgcagct tttcagcatc
     3121 ggcgtcgatt tgctgaatca ggacggaacg cccgtgctcg ataatcaagg caaccgcatt
     3181 ccgagctaca atcaggaaat catcaacaat ttcaccaaag tttttacggg ctggtcgtat
     3241 tgtcaaaatc cagccaattg cccgaactgg cagccgggac tggtcaatta ccgcgacccg
     3301 atgtttgccg tcgccgccaa tcacgacacc ggcgcgaaaa ctctgctgaa ttatgcgggc
     3361 gcgacgccga acattcccgc cgggcaaacg ccggaagagg acatggacgc ggcgcttaac
     3421 aatattttct atcacccgaa cgtcgcgccg tttatcagca agctgcttat ccagcagctt
     3481 gtcaccagca acccgacgcc ggcttatgtc ggtcgcatct cggctgtttt caacgacaac
     3541 ggcacaggca cgcgcggcga tttgaaagcc gttatccggg cgattctgct cgacccggaa
     3601 gcgcgcggca actttaaaac cgaccccgat tacgggaaac tgcgcgagcc ggttttgtat
     3661 atcacaagcg ttctgcgccc gtttaatccg acggcgaaca ataatctcgc cgttccggca
     3721 agctgcaatg ggcagagcga cggcgttatc aacggcatca cgcagccgct cgaacaggac
     3781 gttttcaatg cgccgaacgt ttttaattat tactcgatgg attatattat tccgggcacg
     3841 cctttagccg gaccggaatt cggaattttc tcgaccggaa cggctttgaa acgcccgaac
     3901 ttcgtcaatc agatggcgcc gcccggctcc gcctcgacga ccgccggaat tttagccgtg
     3961 accggaagcg ccgccagtcc gacgagtatt ccctgcggaa cgcggattga tttaagccgc
     4021 ctgcaggcgc tcgccgcttc tgacccgacg ggcgcggcgc tggtcgatac gctcaaccgc
     4081 gaattaatgc acggctcgat gagcgccgcc gtccgcagcg acattttaac cgccgtccaa
     4141 gccgtcgccg ccgccaacac gctgaaacgc gcgcgcaccg cgttttatct ggtgacgacg
     4201 ctgacgcagt atcaggtgca aagatagttg accgagctga ctgaaagttt agtgagttga
     4261 ctgagttatc ggaaacactc aactagctca gtcaactcat ttagaaaaat tatgaaagag
     4321 aacagaagag actttttgaa aaaatcctgc cgcgtcctga gtatgacggc aatcgccacg
     4381 cagatgcggc atttcggatt ggtcaacgta atggcgcagg aaagagcgga cacgcaaaag
     4441 tcggcgggcg gcgatgacta caaggctttg gtctgcgttt ttctcgacgg cggcaatgac
     4501 ggcaacaact ccgtcattcc cagttacgac gaaggctata atcaatacgc ggcggcgcgc
     4561 ggcggcacgt tcggtctggc aatcccgcgt aattcgctgc ttccgataac gccgccgaat
     4621 atgggcgggc aggttt
//
LOCUS       DASWNE010000103         1947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_179872, whole genome shotgun sequence.
ACCESSION   DASWNE010000103 DASWNE010000000
VERSION     DASWNE010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179872"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VGB68_02145"
     CDS             complement(<1..425)
                     /locus_tag="VGB68_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048057.1"
                     /translation="MCCKNFWKKLAPFVLTFLLGLLAANLLTKENPARKETPANISPK
                     NVKCPDKNIDFGGVGSGTGSSDKTESGKIPTSRGGTNQVSVLSTPRANYTDMARQNDT
                     QGVVRVRVAFLASGEIGEVAPVSELPCGLTEQAIAAAKQ"
     gene            complement(535..870)
                     /locus_tag="VGB68_02150"
     CDS             complement(535..870)
                     /locus_tag="VGB68_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048058.1"
                     /translation="MQLKGFLKRALPFFLTFSVGLLVASLFVTIAAPNFNFKKRNHRC
                     REYRENYRLRMENQSLRQNNESLKRQLEDSELKTIPAFDLDVPPPPPPPRVKPNVKLV
                     DPTSPGIIR"
     gene            complement(908..1609)
                     /locus_tag="VGB68_02155"
     CDS             complement(908..1609)
                     /locus_tag="VGB68_02155"
                     /inference="COORDINATES: protein motif:HMM:NF003992.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEY0048059.1"
                     /translation="MKVEKISELTTNRLSVYLRCLTQLAADGIKTISSDGLAKRFHLN
                     SAQIRKDLAYFGEFGVRGVGYYVESLREHLTKILGLDKEHCVAIIGAGRLGTALADYY
                     GFTQTNFSVVALFDADKSKIGQRVGNVRVYDIEDFEKVVEKEKIDVAVIAVPAAFAQG
                     VLEKVSNAGIKAVLNFAPTPLKADDATKLKTVDLTISLESLSYFLAKPAATGTNGGAE
                     KIVKPAHARGRKQKQ"
     gene            1739..>1947
                     /locus_tag="VGB68_02160"
     CDS             1739..>1947
                     /locus_tag="VGB68_02160"
                     /inference="COORDINATES: protein motif:HMM:NF013495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEY0048060.1"
                     /translation="MIAYLSGKLLEKQATSVIIDVGGIGYEVTIPLSTFYELGEEGSD
                     VSLRIYTHVREDALQLFGFKSEREK"
BASE COUNT          476 a          493 c          449 g          529 t
ORIGIN      
        1 atttgcttcg ccgctgcaat tgcctgttcc gtcaagccgc acggcaattc gctgaccggc
       61 gcgacctcgc cgatttcgcc cgaagccaga aaagccacgc gcacgcgaac cacgccttgc
      121 gtgtcgtttt gcctcgccat atccgtataa ttggcgcgcg gtgttgagag aacggacacc
      181 tgattcgtgc cgccgcgaga ggttgggatt ttgccggatt ccgttttatc agacgagcct
      241 gtgccggagc cgactccgcc gaagtcgata tttttgtcag gacattttac gtttttcggg
      301 ctgatattgg cgggagtttc ttttcgggca ggattttctt tggttaaaag atttgccgcc
      361 agcaaaccga gcaaaaaagt taaaacgaac ggagcaagtt ttttccagaa atttttacag
      421 cacatgtttg atttccccgc gcaaaaatta aaccacagat taacacattg aagcaaaatc
      481 ttcatttggt taatctgcgg tctgaagctt aaaagaaatt tttctttatt ttctttatct
      541 gattatgccc ggcgaggtcg ggtcaaccaa tttaacatta ggtttcaccc tcggcggcgg
      601 aggcggcggc ggaacgtcca aatcgaaagc cggaatcgtt ttcaattcgc tgtcttccaa
      661 ctggcgtttg agcgattcgt tgttctggcg caagctttga ttttccatcc gcaggcgata
      721 gttttcgcgg tattcgcggc aacggtgatt gcgtttttta aaattaaaat tcggcgcggc
      781 gatggtcaca aacaagctcg ccaccagcaa accgaccgaa aaggttagaa aaaacggaag
      841 tgctcttttt aaaaatcctt tcaactgcat atttttacct cttgattccc tagttcttaa
      901 ttcaatatca ctgcttttgc ttacgcccgc gcgcgtgagc gggtttcaca attttttccg
      961 cgccgccatt cgtcccggtc gccgcaggtt tcgctaagaa atatgataaa ctctccagcg
     1021 aaatcgtcaa atcgacggtc tttaatttcg tcgcgtcgtc ggctttgagc ggcgtcggcg
     1081 caaaattcaa aaccgcttta ataccggcat tcgaaacctt ttccagaacg ccctgggcaa
     1141 acgcggcggg cacggcaatc accgccacgt caatcttttc cttttcgaca actttttcaa
     1201 aatcttcgat gtcgtaaacc cgcacgttgc cgacgcgctg accgattttc gatttatcgg
     1261 cgtcaaaaag cgcgacgacc gaaaaattcg tctgcgtaaa accgtaataa tcggcgagcg
     1321 ccgtccccaa tcgtcccgcg ccgataatcg ccacacaatg ctctttgtcg agccctaaaa
     1381 tcttcgtcag atgctcgcgc agactttcga cgtaatagcc gacgccgcgc acgccgaatt
     1441 cgccgaaata cgccaaatcc ttgcgaatct gcgccgaatt aagatgaaac cgtttcgcca
     1501 gcccgtccga tgagatggtt tttatcccgt ctgccgcaag ctgagtcagg cagcgcagat
     1561 aaacgctcag gcgattggtg gttagctcgg atattttttc aaccttcatt tctcttgtgt
     1621 gattataacg ccatttctaa aagtgaaaaa gttctcaaat gtgatgatga gcaaaaactt
     1681 tattaacgtt gccctaaagt taggaatcaa ttacaatttg tgtttcaagt gtgaaacaat
     1741 gattgcatat ttatcaggaa aacttttgga aaaacaggcg acgtccgtaa ttatcgacgt
     1801 cggcggaatc ggctatgaag tgacgattcc gctttctact ttttatgagc tgggcgaaga
     1861 aggctcggac gtgtccctgc gcatttacac gcacgttcgc gaggatgctc ttcagctttt
     1921 cggttttaaa tccgagcgcg aaaagca
//
LOCUS       DASWNE010000104         2327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_3536, whole genome shotgun sequence.
ACCESSION   DASWNE010000104 DASWNE010000000
VERSION     DASWNE010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3536"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1753
                     /locus_tag="VGB68_02165"
     CDS             <1..1753
                     /locus_tag="VGB68_02165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0048061.1"
                     /translation="MQTNDKYFQLFTQPTQRLQTLIIGRLMATFLLLVASWFWNSGNV
                     KLSFEDFPRGLFFVFLISVGLTIVYFFVLRLNKNYRWQIRIQFFLDALLITWLVWRTG
                     DLTSPFITLYIVLIGVASFFLPSLETLLTAVLCLLLFVLLSMMVAVAPDYSTGTVPEM
                     TKAIQIISFHTVAFLVVGLLAARLSSRHASGEQLKEATKSLASLRALHERIVQSIRSG
                     LITTDLEGNIYTFNTAAEEITGYKAKQMQGRTIFSLFGSIEQSIVPAVDATREGEPSP
                     RHETDIMTPEGFAVHVGYGISPLFSEAGEMSGLIITFQDLTEVRSMEENVRRKDRLAA
                     VGRVAAGLAHEIRNPLGAMRGAIQVLQSQTPPETAQASLMEIILRESDRLNKIITNFL
                     TYARPRVNNFSEIDVREAIQDTFTLLKHSPDVQPGHGFEFRAPENPVLISADPTQLKQ
                     IFWNLARNSINAMRDGGNLSVELVNLPHKRIQIIFADTGCGMSPEQVERLFEPFSDST
                     TGGTGLGLSIVYQIVRDHGGTINVRSRENDGTTITVELPSDFRSTNGLIESENGDAAT
                     QPSRLESFLTVKSNERN"
     gene            2021..>2327
                     /locus_tag="VGB68_02170"
     CDS             2021..>2327
                     /locus_tag="VGB68_02170"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0048062.1"
                     /translation="MSNLLIVDDEQSYRQLLSLVFEGEGYQVRTATNGFEALEKLQTE
                     PAEVIISDVKMPDMDGIEMLRSVREVLPDVGVVMMTAFASVETAREAFKLGADDFIQK
                     "
BASE COUNT          543 a          600 c          597 g          587 t
ORIGIN      
        1 aatgcagaca aacgataaat actttcagtt atttacgcag cccacgcaaa gattgcaaac
       61 gctgataatc ggacgcctga tggcgacgtt tctgctgctc gtggcgagct ggttctggaa
      121 cagcggcaat gtaaaactgt cgttcgagga ttttccgcgc ggtttatttt ttgttttcct
      181 gatttcggtc gggctgacga ttgtttattt tttcgtcctg cggctcaaca aaaattatcg
      241 ctggcagatt cggattcaat tttttctcga cgctctgctg ataacgtggc tcgtctggcg
      301 aacgggcgat ttgacctcgc cgtttatcac gctttatatc gttttaatcg gcgtggcaag
      361 cttttttctg ccttcgctgg aaacgcttct gacagccgtt ttgtgtcttt tgctgtttgt
      421 tttgctctcg atgatggtcg ccgtcgcgcc ggattattcg accggcacgg ttcccgaaat
      481 gacgaaggcg attcaaatca tcagttttca cacggtcgct tttcttgtcg tcgggctttt
      541 ggcggctcga ttatcgtcgc gccacgcttc gggcgagcag ctgaaagaag cgaccaaatc
      601 gctggcaagc ctgcgcgcgc tgcacgaaag aatcgtccaa tcgattcgct ccggtttaat
      661 aacgaccgat ttggaaggaa atatttacac tttcaacacc gccgccgaag aaatcaccgg
      721 ctacaaagcg aaacagatgc agggcagaac gattttttcg ctcttcggca gcatcgagca
      781 atccatcgtg ccggcggtcg acgcgacgcg ggaaggcgag ccttcgccgc gccacgaaac
      841 ggacattatg acgcccgaag gtttcgccgt tcacgtcggc tacggaattt cgccgctctt
      901 tagcgaggcg ggcgagatga gcggcttgat tatcacgttt caggatttga ccgaggtgcg
      961 ctcgatggaa gaaaacgtcc gccgcaaaga ccgcctggcg gctgtcggtc gcgtcgccgc
     1021 cggcttggcg cacgagattc gcaacccgct cggcgcgatg cgcggcgcga ttcaggttct
     1081 gcaatcgcag acgccgcccg aaacagcgca agccagttta atggaaatca ttctgcgcga
     1141 atccgaccgc ctgaacaaaa tcatcaccaa ttttctgact tacgcccgtc cgcgcgtcaa
     1201 caacttttcg gaaatcgacg tgcgcgaagc cattcaggat acgtttacgc ttttaaaaca
     1261 cagtcctgac gtgcagccgg gtcacggctt cgaatttaga gcgccggaaa atccggtttt
     1321 gatttcggct gacccgacgc agctcaaaca aattttctgg aatctggcgc gaaactcgat
     1381 taacgcgatg cgcgacggcg gaaatttgtc tgtcgagctg gtcaatctgc cgcacaagcg
     1441 catccagatt atctttgccg acaccggctg cgggatgtcg cccgaacagg tcgagagact
     1501 tttcgagcct ttttccgatt cgacgaccgg cggaaccggt ctcggacttt caatcgttta
     1561 tcaaatcgtc cgcgaccacg gcggcacgat aaatgtccgc agccgcgaaa acgacggcac
     1621 gaccatcacg gtcgagctgc cgagcgattt tcgcagcaca aacggcttga ttgaatcgga
     1681 aaacggcgac gcggcgaccc agccgtcgcg gctcgaaagt tttctgaccg tcaaatcaaa
     1741 tgagagaaat taaggagaaa ggcaaaagat aaaaggcgaa agtgcgaacc attgcccgga
     1801 aagttataat cgatgcccgg aatgatgaaa aataaaatgc gattttatct gttgccgctt
     1861 tattctcgtt tcttattttc gcctttttcc ttttgctctt tttctttaat ttaaattctt
     1921 cttgaaaatt aacacggttt tctgtgaaaa tagatacggc taaactttcg gctatccatc
     1981 attttcaatt cccgctcgct ttttaaataa taaatttcca atgtcaaatt tactgattgt
     2041 tgatgacgaa caaagttaca ggcagctttt aagcctcgtt ttcgaaggcg aaggttatca
     2101 ggtgcgcacg gcgaccaacg gctttgaggc attagaaaaa cttcaaaccg aaccggctga
     2161 ggtgattatc tcagacgtga aaatgcctga tatggacggc atcgagatgc tgcgctccgt
     2221 gcgcgaggtt ttgccggacg tcggcgttgt gatgatgacg gctttcgcct cggtcgaaac
     2281 cgcgcgcgag gcgttcaagc tcggagccga cgatttcatt caaaagc
//
LOCUS       DASWNE010000105         4980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_463135, whole genome shotgun sequence.
ACCESSION   DASWNE010000105 DASWNE010000000
VERSION     DASWNE010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463135"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VGB68_02175"
     CDS             complement(<1..221)
                     /locus_tag="VGB68_02175"
                     /inference="COORDINATES: protein motif:HMM:NF013492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY0048063.1"
                     /translation="MAIRKITEYPEEVLAKTGAPVTQFDEELEKLCADMFETMYDAEG
                     VGLAAPQIGLNLRLFVMDCEGIKLVAANP"
     gene            complement(319..534)
                     /locus_tag="VGB68_02180"
     CDS             complement(319..534)
                     /locus_tag="VGB68_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048064.1"
                     /translation="MTEINDIRNNAQAKEPVRCTKCDTIIDHYNTFLSPTNETNDVCT
                     ECLLREDKGFNTKSDWKRASRRGVIPR"
     gene            complement(531..2156)
                     /locus_tag="VGB68_02185"
     CDS             complement(531..2156)
                     /locus_tag="VGB68_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048065.1"
                     /translation="MNHTDAETQRRDEEKTSFTASPRLRFPAFLILLHLAVTLPLAYW
                     LNIWMDEASTLYTTQNGFFQTFQNVFADEKQAPLYFLLLSLWRTLDGSIFFARLLSIV
                     FSCLAIRFFCGLARSFFDESPARFISLLFALHPFLIWSSLEIRGYSLVILLSVLLLKF
                     FEENFYRTEKEKQLKQDEQRRRDKIFSSSALTGENPRAFFVLTAIAALYTNYYLGFLL
                     AGCFAALPVLKKFKQARAYFLQMLIVGLAILPLLWIIKEQLDVNANYFQADKSFVEGA
                     KIIWNHGLSLTFPTEFSPQSESSAVSFIRIWLVRFGIFLTCFLLARNGFRQINKDISL
                     FGIISIVIVAFLLFAYFLLGGVYVALRHTAVLLVPLFIFVPLLLSAVLPRRSWIFFAV
                     VFAFLYPYSVYKNYPQMAKRGDWIRVARFIEANEAPNQPIIVFQNYDALSLPFHYKGV
                     NRILPDENFFAWSFEENPSSPAALQKQIEFVISEIPPGAGEIWLATEEICQDAKTRIA
                     CQPLENFVQANYTIEKQKDFYLERVRLLRKKPR"
     gene            complement(2153..2989)
                     /locus_tag="VGB68_02190"
     CDS             complement(2153..2989)
                     /locus_tag="VGB68_02190"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0048066.1"
                     /translation="MNCPLCSSNSKEIFAAKGFPVRDCIECRHRFAEVSTDGNHVAEI
                     YDDTYFNGGGAGYSDYLAEGEMLRRRGRDYAKILSKYAKAGKILDVGAAAGFILQGFC
                     DAGWTGTGIEPNASMARAGSEKFDLKIFQGDFENFQSAEKFDAVSMIQVAAHFHNPRN
                     AFENAFELLKPGGVLLVETWNCRSLTARLFGRAWHEYSPPSVLHWFSPKTLSGFLREI
                     GFEKLAQGRPSKKISGEHAKSLLRYRLGNKFDSFLKLIPGKINFPYPAEDLFWALYQK
                     QK"
     gene            complement(3077..4600)
                     /locus_tag="VGB68_02195"
     CDS             complement(3077..4600)
                     /locus_tag="VGB68_02195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY0048067.1"
                     /translation="MKKLPTVFLLIFALGALIRFIDVWRPVERASWRECDLASISRNY
                     AREGMNPFYPRIDWRGTSAGYAEMEFPVYPYLTALFYRLFGFNEYIPRLINFLFSLVA
                     LWFFFRLARSFLDDWAAIFAFAFFAFHPLVVEISTSIQPEGLMLLCYTASVYFFGGWL
                     KSDSNRDFVFASIFTALAILAKAPAAHIGLLFGVLLLQKFGLGVFRQSKVWLFGIFSL
                     LPAALWYLHAKSLWKNYGNSLGVSNETHLVGADFFTNSYFIKGILSAEIFNVWLGFGL
                     VLGIFAVWRGRREKVVFLCLLWLASVFAFYIIAARTTADDWAVYYHIFSVAPAALLFG
                     FGARKFWEFFRNYYNFSSQLDAFAKLKQISLVLLLAISVAATFLLEAKGVRANFLQHR
                     VPDESFACAKEFKPYLKTEGLILASGGNCFDADGLPVAYNASFMFYWLDRKGFNVCTE
                     QQSLAKVREFASQGAKYFVAQKNLLAEKPNLENELRENFAVVAECEAMILFELKVSY"
     gene            complement(4774..>4980)
                     /locus_tag="VGB68_02200"
     CDS             complement(4774..>4980)
                     /locus_tag="VGB68_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048068.1"
                     /translation="NLLITLPFVVYGVFRYLFLVHKRKEGGDPVQLLFRDRASVINIV
                     LWILSVCAVIYLPAFWNKSLSFLR"
BASE COUNT         1421 a         1264 c         1189 g         1106 t
ORIGIN      
        1 tcgggattgg cggcgacgag cttgatgccc tcgcaatcca tcacgaaaag ccgcagattc
       61 agcccgattt gcggcgcagc cagcccgacg ccttccgcgt catacatcgt ctcgaacata
      121 tcggcgcaca atttttccag ctcttcgtca aactgcgtga cgggcgcgcc cgtttttgcc
      181 agaacttctt cgggatattc ggtgattttt ctgattgcca tagataaaat tacgaattac
      241 gaattacgaa ttacgaatta cgaataaaga aaagattctt caattcgtaa ttcgtaattt
      301 gtaattcgta attgattatc atcgcggaat gacgccgcgg cgtgaagccc ttttccagtc
      361 gcttttcgta ttaaaccctt tatcttcacg caaaagacat tccgtgcaaa cgtcgttcgt
      421 ttcgttagtc ggacttaaaa aagtattgta atggtcgata atggtatcgc atttagtgca
      481 cctgaccggc tctttggctt gcgcgttgtt tctgatgtcg ttgatttcag tcatctcggt
      541 tttttcctca agagtcgaac tctttccaaa taaaaatcct tctgcttttc aatagtgtaa
      601 ttggcttgga cgaaattttc caaaggctgg cacgcaatgc gcgttttcgc gtcctggcag
      661 atttcttcgg tcgccagcca gatttccccg gctccgggcg gaatttccga gatgacaaac
      721 tcgatttgtt tttgcagagc cgccgggctc gaaggatttt cttcaaagct ccaggcgaaa
      781 aaattttcgt cgggcaaaat ccggttcacg cctttgtaat gaaacggcag cgaaagcgcg
      841 tcgtaatttt gaaaaacgat aatcggctgg ttcggcgctt cattcgcttc gataaatcgc
      901 gcgacgcgaa tccaatcgcc gcgctttgcc atctgcggat agtttttata aacggaatac
      961 ggatacagga aagcaaaaac gaccgcgaaa aaaatccagc tccggcgcgg caaaacggcg
     1021 cttaaaagca gcgggacgaa tataaaaagc ggcactaata aaaccgccgt atgccgcagc
     1081 gcgacataaa ctccgccgag cagaaaataa gcgaaaagca aaaaagcgac gatgacaatc
     1141 gagataatgc cgaacagtga aatatcttta ttaatttgcc ggaaaccgtt tcgggcaagc
     1201 aaaaaacacg tcaggaaaat gccgaaacgc accagccaga ttctgataaa ggaaacggct
     1261 gaagattccg attgcggcga aaactcggtc ggaaaagtca ggctcaaacc gtgattccag
     1321 attatcttcg cgccttcgac aaaggattta tcagcctgaa aatagtttgc attaacgtca
     1381 agctgctcct ttattatcca gagcaaaggc agaatcgcca gcccgacaat cagcatttgc
     1441 aaaaaataag cccgcgcctg cttaaacttt ttcaagaccg gcagcgcggc gaaacagccg
     1501 gcgagcaaaa aaccgagata ataattcgtg taaagcgcgg caatcgccgt caaaacaaaa
     1561 aacgcccgcg gattttcgcc ggtcaacgca gatgaagaaa agattttatc ccgccgccgc
     1621 tgttcatcct gtttcaattg cttttctttt tccgtgcggt aaaaattttc ttcaaaaaat
     1681 ttcaaaagca gaaccgaaag cagaatcacc agcgaatagc cgcgaatttc caggctcgac
     1741 caaatcaaaa acgggtgcag cgcaaacaaa agggaaatga agcgcgccgg gctttcgtca
     1801 aaaaaactgc gcgccaatcc gcaaaaaaac ctgattgcca gacagctgaa aacgattgat
     1861 aaaagccgcg cgaaaaaaat cgagccgtcg agcgtgcgcc ataaactgag cagcagaaaa
     1921 taaagcggcg cttgtttttc gtcggcgaaa acgttctgaa aagtttgaaa aaatccgttt
     1981 tgcgtcgtgt agagcgtcga cgcttcgtcc atccagatgt tcagccaata agccagaggc
     2041 agcgtcacgg cgagatgcag cagaatcaga aacgcgggga aacgcagacg cggcgacgcg
     2101 gtgaacgagg ttttttcttc atctcttcgc tgtgtctcgg cgtctgtgtg gttcattttt
     2161 gcttttgata taaagcccag aataaatctt ccgccggata cggaaaattt attttaccgg
     2221 gaattaattt caaaaacgaa tcgaatttat ttcccagccg ataacgcaaa agcgatttgg
     2281 cgtgctcgcc cgaaatcttt tttgaagggc gaccctgagc gagtttttca aaaccgattt
     2341 cccgcaaaaa gccggataaa gtctttggcg aaaaccagtg cagaacgctc ggcggcgaat
     2401 attcgtgcca ggcgcgcccg aaaaggcgtg ccgtcaggct tcggcaattc cacgtttcga
     2461 cgagcagcac gccgcccggt tttaataact caaaagcgtt ttcaaacgcg ttgcgcggat
     2521 tatgaaaatg cgccgcgacc tgaatcatcg aaaccgcgtc gaatttttcc gcgctttgaa
     2581 aattctcgaa atcgccctga aagattttta aatcgaattt ttcgcttccg gctctcgcca
     2641 tcgaagcgtt cggctcgatg cccgtgcccg tccagcccgc gtcgcaaaag ccctgcaaaa
     2701 taaaacccgc cgccgcgccg acgtcgagaa tttttcccgc ctttgcgtat ttcgacaaaa
     2761 ttttcgcgta atctcgcccg cgccgccgca gcatctcgcc ttccgccaga taatcggaat
     2821 aacccgcgcc gccgccgttg aaatacgtat cgtcgtagat ttccgcaacg tgattgccgt
     2881 cggtcgaaac ctcggcaaaa cgatgccggc actcgatgca atcgcgcacg ggaaaacctt
     2941 tcgccgcaaa gatttctttg gaatttgacg agcaaagcgg acagttcata aattcgtcat
     3001 tcgtcgttcg tcctttgttc tttgccgttt ttaaaccgcg ttcggaaatt tcattcaaac
     3061 ggcaaagaac aataaactaa taactaacct ttaattcaaa taaaatcatc gcctcgcatt
     3121 cggcgacgac cgcgaaattt tctcgcagtt cattttccag attcggcttt tccgccagaa
     3181 gatttttctg cgcgacgaaa tattttgcgc cttgcgaggc aaactcgcgg actttggcga
     3241 gcgattgctg ctcggtgcag acgttaaagc ccttgcggtc gagccagtaa aacataaacg
     3301 acgcgttgta ggcgacgggc aagccgtcgg cgtcgaaaca gtttccgccg ctggcgagaa
     3361 tcaagccttc cgttttcaga tacggcttaa actccttggc gcaggcaaat gattcatcgg
     3421 gaacgcgatg ctgcaaaaaa ttggcgcgca cgcctttggc ttcgagcaga aacgtcgcgg
     3481 caacggaaat cgccagcaga agaaccagcg aaatttgttt taatttggcg aaagcgtcga
     3541 gctgagagga aaaattgtaa taattgcgga aaaattccca aaactttctc gcgccgaagc
     3601 cgaaaagaag cgcggcgggc gcgaccgaga aaatatgata ataaaccgcc caatcgtcgg
     3661 cggtcgtgcg cgccgcgatt atataaaacg cgaaaaccga agccagccac aaaagacata
     3721 gaaacacgac tttttcgcgc cgcccgcgcc agacggcgaa aattcccaaa accaagccga
     3781 agccgagcca gacgttgaaa atctccgccg acagaatgcc ttttataaaa taggaattcg
     3841 tgaaaaaatc cgcgccgacc agatgcgttt cgttggagac gccgagcgaa ttgccgtaat
     3901 ttttccagag acttttcgcg tgcagatacc aaagggcggc gggcagcagc gaaaaaatcc
     3961 cgaacagcca gactttgctt tgtctgaaaa cgccgagccc gaatttttgc agcagcaaaa
     4021 cgccgaaaag caatccgatg tgcgccgccg gagctttggc taaaattgcc agggcggtga
     4081 aaatcgaggc gaaaacgaaa tccctgtttg aatcgctttt cagccagccg ccgaaaaaat
     4141 agacgctcgc cgtataacac aaaagcatca agccttccgg ctgaatcgaa gtcgagattt
     4201 cgacgaccag cggatgaaaa gcgaaaaaag cgaacgcgaa aatcgccgcc caatcgtcca
     4261 gaaagctgcg cgccagacga aagaaaaacc agagcgcgac gagcgaaaaa agaaaattta
     4321 tcaggcgcgg aatgtattcg ttaaaaccga aaagccggta aaagagcgcc gtcagataag
     4381 ggtaaaccgg aaattccatt tcggcgtagc ccgcgctcgt tccgcgccaa tcgatacgcg
     4441 ggtaaaacgg gttcattcct tcgcgagcgt aattgcggga aattgaagcg aggtcgcact
     4501 cgcgccacga ggcgcgctcg accggtcgcc aaacgtcgat gaagcggatc agagcgccga
     4561 gcgcgaaaat taaaagaaaa actgtcggga gctttttcat tggctgattt ttcaacacaa
     4621 agacacgagg aacacaaagg aaaattcaaa gcggactttg cggcttgatt gacaaaatga
     4681 aatcattttc taaatccgcg tttatctgtg tcaatctgtg gtttcatttt tctttctttg
     4741 cgctgctcgg cgtctttcgc gtcgaaaatt tctttagcgc agaaagctca gagatttatt
     4801 ccagaacgcc ggcagataaa tcaccgcgca gaccgacaga atccagagca cgatattaat
     4861 aaccgaggcg cggtcgcgaa acagcaattg aaccgggtcg ccgccttcct tgcgcttgtg
     4921 caccagaaac agatagcgaa aaacgccgta gacgacaaac ggcagcgtga tgagcagatt
//
LOCUS       DASWNE010000106         3287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_640090, whole genome shotgun sequence.
ACCESSION   DASWNE010000106 DASWNE010000000
VERSION     DASWNE010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640090"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(433..1050)
                     /locus_tag="VGB68_02205"
     CDS             complement(433..1050)
                     /locus_tag="VGB68_02205"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0048069.1"
                     /translation="MINARELFGDIDIYLFDQILKNQFSSQMSILDAGCGAGRNLIYF
                     LRSGCKIFGVDRNPEAIRYVRELAQSLAPELSAENFQISKVEEMPFPDESFDAVISSA
                     VLHFARSEQHFNGMLAEMWRVLKPEGLLFVRLASSIGIEDKIKLIEGRRYNLPDGSER
                     FLVDEELLLSATSRLGGILSEPLKTTNVQNLRCMTTWVLRKEAAR"
     gene            1259..2485
                     /locus_tag="VGB68_02210"
     CDS             1259..2485
                     /locus_tag="VGB68_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048070.1"
                     /translation="MNVFFRSGFSGLILLFVFLLIAENVLADAPGNSCGGVGGTIRSQ
                     DRTIPQRGGGTLNAKIFAPDAALQTAPCPLITMLPGGGAEITSVEWAARRLAAAGYVV
                     IITKPQSGGGLNDYNTAAISGIDFVVSDANPYLSGTDVNAVGAAGWSLGARVLTRTQE
                     EDTRISAVVEWDNLAAGETGDEGSPQCTNQPATLRVPRVPALGQASDTCIDGRGANAK
                     KTAFNRWRQFGQPVMQVVFRGANHFWWSASASNNNQYDIADYYTKNWFDRWLKGDLTA
                     TGRLLSRTVVNNTALELLLSQNFNSGVFFDGYNCEDLRAACPPSAATATLGGRVTNSS
                     GRGIACARGALNGDYLSAPRYALTNSSGYYRFVNVPVYFRYQATVFAKNRTFQQTSVT
                     LTVTGDASSLNFTSNQ"
     gene            2879..>3287
                     /locus_tag="VGB68_02215"
     CDS             2879..>3287
                     /locus_tag="VGB68_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048071.1"
                     /translation="MTDKCMQPEDIEVIFSKPGVSWSNDDRYHVRIWLSEPPQRRKIL
                     ARGARKLGNNSTPEDAEDAWQDFNIHYSAKRQCKWESSLEGVMRKYDPNSKKGRPFCV
                     YLLFSFGRFCIRQKQSRSRPNSEFQIDNIMDNSD"
BASE COUNT          796 a          805 c          851 g          835 t
ORIGIN      
        1 ttccagatgt ttcaaagctt tagcgcgagc cgttgaattg ttcgtttact tttacaatga
       61 gcgacagctc agaaaaagac gcgctccaaa gtatccggca ttcttagcag aaaacttgcc
      121 aacgctcatt tagtcactcc ctgtttatcg ggagcggcta aataagcgtc ggcggatttt
      181 ccgttaagaa tgccggatgc tccggcaagc gtcttttcgt gagtcgtcgg tcattataaa
      241 aataaacaat aaaaataaac aaacaattcg acggctctcg ccgaagcttt gaaacatatc
      301 gagaaacaac gcgcggcgcg gtgcaatctc gccggataat gccgaaggta taggttctgc
      361 cgcaagtccg tctgtctaat cgagccgccg gataaatctc ccgccgtttc tctgcgaaat
      421 tcgattgctt cgttaccggg cggcttcctt gcgcagaacc caggtcgtca tacagcgcag
      481 attctgcacg ttcgtagttt tgagcggttc agataaaatt ccgcctagcc tgctcgtcgc
      541 cgaaagcagc aattcttcat cgaccagaaa acgctccgag ccgtccggca gattgtagcg
      601 gcgtccttca atcaacttga ttttatcttc gatgccgatt gaagaggcaa gacggacgaa
      661 caacaaaccc tccggtttga ggacgcgcca catttccgca agcattccgt taaaatgctg
      721 ctcgctgcga gcgaaatgaa gaacggcgct gctgataacc gcgtcgaagc tttcatccgg
      781 aaacggcatt tcttctactt ttgaaatttg aaaattttcg gcggaaagct caggagccag
      841 agattgcgcc agctcgcgaa cataccgaat cgcttccggg ttccggtcaa cgccgaaaat
      901 tttacatccg ctgcggagaa agtatataag attgcgtccc gcgccgcatc cggcatcgag
      961 aatgctcatt tgactactga attgattctt taatatctgg tcgaacaggt aaatatctat
     1021 atcgccgaat aattcccgcg catttatcat ttgctaagtt tagtttaaat ttccgctcga
     1081 cgggtgattt cccggcattt taaagtgccg gcaataagcg cggctcgcga tagatttcgc
     1141 gcgaagggta acgattaatg caagtttttc aaacttttac ttttttacgg tactctaaat
     1201 atcgtatttt ccggttactg aatgaaaccg gctgtaaacg ctcttcggga aagaatttat
     1261 gaacgttttt tttcgctcag gtttttcggg tcttattctg ctgtttgttt tcctgttaat
     1321 cgccgagaat gttttagccg acgcgcccgg aaattcgtgc ggcggcgtgg gcgggacgat
     1381 tcgctctcag gataggacga tcccgcagcg cggcggcgga acgctgaacg ccaaaatctt
     1441 tgccccggac gccgctttgc aaaccgcgcc ctgtcccctg atcacgatgc tgcccggcgg
     1501 cggcgcggaa attacgagcg tcgagtgggc ggcgcgacgt cttgccgccg ccggatacgt
     1561 cgtcattatc accaagccgc aatccggcgg cgggctcaac gattacaaca cggcggcgat
     1621 cagcggcatc gattttgtag tgtcggacgc caatccgtat ttgagcggca ccgacgtgaa
     1681 tgccgtcgga gctgccggct ggtctttggg cgcgcgggtt ttgacgcgca cgcaggaaga
     1741 agacacgcga atctcggcgg tggtcgaatg ggataattta gccgccggcg aaacaggcga
     1801 cgagggttcg ccgcaatgca ccaaccagcc ggcgacgttg cgagtgccgc gcgttccggc
     1861 gctcggtcag gcttccgaca cgtgtatcga cgggcgcggc gcgaacgcca agaaaaccgc
     1921 ctttaatcgc tggcggcagt tcggacagcc cgttatgcag gtggtttttc gcggcgcgaa
     1981 tcatttttgg tggagcgcga gcgcgagcaa taataatcaa tacgacatcg ccgattacta
     2041 tacgaaaaac tggttcgatc gctggctgaa aggcgatttg acggcgaccg gcagactgct
     2101 gtcgcgcacg gtcgtcaaca acacggcgct cgaactcctg ctcagtcaaa actttaattc
     2161 gggcgtgttt ttcgacggct ataactgtga ggatttgcgt gccgcctgcc cgccgtcagc
     2221 cgcgacggca acactcggag ggcgcgtgac gaactccagc gggcgcggca tcgcctgcgc
     2281 ccgcggcgct ttaaacggcg attatctttc agctccgcgg tacgctttga ccaactcatc
     2341 gggttattac aggtttgtta atgtgccggt ttattttcgc tatcaagcga cggttttcgc
     2401 taaaaaccgc acatttcagc aaacgtccgt gacattgacc gtcaccggag acgcgtcgag
     2461 cttaaacttt acgagtaatc aataaaaggg cttgccggaa attaccggat ttgatttcga
     2521 tatttaaagt tctcctttaa cgactgcctc gccgccgcgg cgatgccttc gtctccaaca
     2581 ctatttcaat tctcgacgat gtcggagata agttttcttt gtttgttaca ttacggctgt
     2641 cggcgcgttt tttgaacggc tcaagtccgt ttttgaaacc aatgtttttg tttaaaaaag
     2701 agttttcagg tgcttgcgaa ataagtcggc tgatttagat ttgacgaaaa tgttatttaa
     2761 aaggatgttt gcctgtagct ttttggtttc tattgcctag tagtatataa atcaataccg
     2821 cacgttgaac ggcgaagtta aaggaaaagg aagttaaagg aaaaggagga gtgctgtgat
     2881 gactgataaa tgtatgcagc ctgaggatat agaagtaata tttagtaagc ctggggttag
     2941 ttggtcaaat gatgatcgat atcacgtccg gatatggtta agcgagcctc cgcagcggag
     3001 aaaaatactg gcgcgcggcg cgcgcaagct tggcaataat tcaactcctg aagacgctga
     3061 agatgcctgg caggatttta atattcatta ttctgccaaa cgtcaatgca aatgggagtc
     3121 ttcacttgaa ggtgtgatgc gcaaatatga cccgaattcg aaaaaaggac gccctttttg
     3181 tgtttatcta ctgtttagtt tcgggaggtt ttgcatcaga caaaaacagt cccggtcccg
     3241 accaaacagt gagttccaga tagataatat tatggataat tccgata
//
LOCUS       DASWNE010000107         1645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_463169, whole genome shotgun sequence.
ACCESSION   DASWNE010000107 DASWNE010000000
VERSION     DASWNE010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463169"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VGB68_02220"
     CDS             <1..720
                     /locus_tag="VGB68_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048072.1"
                     /translation="AGLPLVLLSMLALFPPPTDELGTFSEMSMVYAAIYGGLILRTLI
                     FFGCAWVFMNLFRGEVVDRSLHYYFLAPVRREVLVVGKYLSGLIATSVLFSITTIGSM
                     FIYYFWLFPSESARFFFDGAGAGQMLAYLGVTILACIGYGAVFLIVGLFFRNPIIPGL
                     MLYGWEWLNFLLPPLLKKLSVIHYLHSLVPVPMSEGPFAVLVEPTPAWISVPSLLLFT
                     GVVLFLASLHIRRMEISYAGD"
     gene            707..1279
                     /locus_tag="VGB68_02225"
     CDS             707..1279
                     /locus_tag="VGB68_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048073.1"
                     /translation="MRATKAKDMRADEFLPLRASSIRQVWNVRRAAFLLSSFLGLTLL
                     LSCSFGCAPSSSQVDGKQSPPGSASSDLPEITDETLRQEINDAYLREVPEENGAGEPI
                     SWRFDEDEPKEFTVVDKQIEGERATIIIDIKTGSAPGARAPKSLAGQIRTKWELQKGW
                     ALRTWEIVESENISMKYKNLTKPPALNSDR"
BASE COUNT          369 a          425 c          460 g          391 t
ORIGIN      
        1 gcgggattgc cgctcgtcct gctgtcgatg ctcgcgcttt ttccgccgcc gacagacgag
       61 ctgggaacct ttagcgaaat gagcatggtc tatgccgcga tttatggcgg gcttatcctt
      121 cgcacgctca tatttttcgg ctgcgcctgg gtttttatga atttatttcg cggcgaggtg
      181 gtggacagaa gcctgcacta ctatttcctc gcgcccgtcc ggcgcgaagt ccttgtcgtc
      241 ggcaaatacc tgtcgggact catcgcgacc agcgtgttat tcagcattac gaccatcggg
      301 tcgatgttca tatattactt ctggctcttt ccgtcagagt ccgcgcgatt ctttttcgac
      361 ggcgcgggcg ccggtcagat gctggcttac cttggagtca caatccttgc gtgcattgga
      421 tacggcgcgg tctttctgat cgtcgggctg tttttccgca acccgattat tcccggtctg
      481 atgctctacg gctgggagtg gctcaacttt ctgctgccgc cgctgctgaa gaagttaagc
      541 gtgattcatt acctgcattc gctcgtgccc gtgccgatgt ctgaaggtcc tttcgcagtg
      601 ctcgtcgaac cgacgcccgc ttggatttcc gtgccgagtc ttcttctgtt taccggcgtc
      661 gtgctctttc tcgccagcct gcacatacgc cgtatggaga ttagctatgc gggcgactaa
      721 agcgaaagat atgcgcgcgg atgaattttt gccgctgcgg gcgagttcga ttcggcaggt
      781 ctggaatgtg cggcgcgcgg cattcctgct ttcatcattt ttaggattaa cgctgctgct
      841 aagctgctcg ttcggctgcg cgccgtcttc ttcgcaggtc gacggcaaac agagtccgcc
      901 cggctccgcg tccagcgact tgcccgaaat tactgacgaa acgctccggc aggagattaa
      961 cgacgcttat ctccgggaag tgccggaaga gaacggagcg ggcgaaccga tcagctggag
     1021 atttgacgaa gatgagccga aggagtttac cgtcgtagat aagcagattg aaggtgagcg
     1081 ggcgacaata atcattgaca tcaaaaccgg ctcggcgccg ggcgcgcgcg cgccgaaatc
     1141 cctggcgggg cagattcgca cgaagtggga actgcagaag ggctgggcgc tcagaacctg
     1201 ggagattgtg gaaagtgaga atatttcgat gaagtataaa aatctcacga agccgcccgc
     1261 gctgaattcc gaccgctgat tcctttatga aaaactcgac ggagtttatt ttgcgattga
     1321 cgggagaaaa cagcaagaca tcagccgcga aataacacgc cgcgaaataa cgttaagaaa
     1381 gatacgaaac cggaaagcct caacgctctg gaaaaaaagg cgctcgacct taaagacccg
     1441 tcaaatctgg cgagcagaat cagatggttc aaaaaaactt cgagcgttga agaaagcgtc
     1501 gatagaggaa atatgattgt cgcaatccgc agagtacatc tgatttttga cgatttttgc
     1561 ccgaagacgc gagctttccg gcgcaggcga aatctttatc tatcatctgg aacaaaataa
     1621 atgccgaaat cagacccgaa ggatt
//
LOCUS       DASWNE010000108         2017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_251094, whole genome shotgun sequence.
ACCESSION   DASWNE010000108 DASWNE010000000
VERSION     DASWNE010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251094"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VGB68_02230"
     CDS             <1..514
                     /locus_tag="VGB68_02230"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEY0048074.1"
                     /translation="IFNFVGSGATTGRGITDERAHAGGFLYIDNTTVEKNVQSNLFVG
                     PTGGGVNVFVNNSRFLGSTANSGAAIRGGSKASITKSSFSGNLNFGIVVEADTAGTFA
                     AIDSCVITNNGTGVVTTTNSPTAQIANNLISGNGTGVQNFSGTLRSTNNRIDGNTTNV
                     SGILSGYNVQ"
     gene            complement(669..941)
                     /locus_tag="VGB68_02235"
     CDS             complement(669..941)
                     /locus_tag="VGB68_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048075.1"
                     /translation="MTEIIGWASSIILLATLIKQVYKQWKEGTGEGVSKWLFVGQVFA
                     SIGFTVYSYMVGNWVFTVTNAILTVNNFIGLGLSFYFRRRDRDKSD"
     gene            complement(1177..>2017)
                     /locus_tag="VGB68_02240"
     CDS             complement(1177..>2017)
                     /locus_tag="VGB68_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0048076.1"
                     /translation="PYWVTFPEIVKFCRAESVFIETEETDFVLNAEQVKAAITDKTRL
                     LIVNSPNNPTGRVIAAAEMRKIVEACAEKNVYVLTDECYLFFAYPPSEVFTSAALPDE
                     LRRFVCVAGSFSKTYAMTGWRIGYTIADAEWTRAMVKLQSHSATHPTSFVQYACARAM
                     QQREKSMATVDAMLAEYGKRRDWLIPALREINGFRCAMPEGAFYAFVDVRDLLGERFE
                     NSCAVAEHLLREAQTVVTDGAGFGAEGFLRLSYATSLENLQTAIERMKNLFNSKSDAT
                     ANV"
BASE COUNT          507 a          545 c          500 g          465 t
ORIGIN      
        1 gattttcaat tttgtgggtt cgggcgcgac caccggacgc ggaatcaccg acgagcgcgc
       61 gcacgcgggc ggcttcctgt atatcgacaa cacgaccgtc gagaaaaacg tccagtcgaa
      121 tcttttcgtc ggtccgacgg gcggcggcgt gaatgttttt gttaataatt cgcgcttttt
      181 gggcagcacc gccaactccg gcgcggcgat tcgcggcggc agcaaagcgt ccatcacgaa
      241 atcgagcttc tccggcaacc tgaacttcgg catcgtcgtc gaagccgaca ccgccggcac
      301 gtttgcggct atcgacagct gcgtcattac caacaacgga accggcgtgg tgaccacgac
      361 caactcgccg accgcccaga ttgccaacaa cctgatctcg ggcaacggca cgggcgtgca
      421 gaacttcagc ggcacattgc gctcgaccaa taacagaatc gacggcaaca ccacgaacgt
      481 tagcggaatt ctgagcggtt ataacgtcca gtaacaaatc ggtcggacaa tgaaatgcaa
      541 agattcagac aaaatgtttc tttgcttttc atcgttaaaa gcagtgaacg aaagacgcag
      601 aaaagaagaa aagcatttaa acactcttct tttctgcgtc tttcgcgcat ttcgcgggct
      661 gattctgttt aatcggattt atcgcggtca cggcggcgaa aataaaaaga aagtccgaga
      721 ccgataaaat tattcaccgt caaaatcgcg ttcgtcacgg taaaaaccca gttgccgacc
      781 atatagctgt aaaccgtaaa accgattgaa gcgaaaacct gacctacgaa aagccatttc
      841 gagacgcctt cgcccgtgcc ttccttccat tgtttataaa cctgtttgat gagtgtcgcg
      901 agcaagatta tcgagcttgc ccagccaatt atttcagtca tagttaagga tattaattaa
      961 aaataaagat atttcttacc cgctcccggc agccagccgc cgccgcagtt acagcttatc
     1021 gccttcgata ataatttatg gtcgtacaaa ttctttatga attccagtgc cgtcacaact
     1081 ttatatgttt catcaaagga aattaaaaag gctcgcggtc ggcagcaaat tcattaatcg
     1141 tctttcgagc tttttccggt aattcccgta aagttttcat acatttgccg tcgcgtccga
     1201 tttcgaatta aataaatttt tcattcgctc gattgccgtt tgcaggtttt ccagcgacgt
     1261 cgcgtaggaa agccgtaaaa agccttccgc gccgaaaccc gcgccgtcgg tgacgaccgt
     1321 ctgcgcttca cgcaaaagat gttcggcgac ggcgcaggaa ttttcgaacc gctcgccgag
     1381 caaatcgcgc acatcgacga aagcgtaaaa cgcgccttcg ggcatcgcgc acctgaaacc
     1441 gttaatttcg cgcagcgccg gaatcagcca gtcgcggcgc ttgccgtatt ccgcgagcat
     1501 cgcatcgacg gtcgccatcg acttttcgcg ctgctgcatc gcgcgcgcgc aggcgtattg
     1561 gacgaaactg gtcgggtgcg tcgccgagtg gctttgcagc ttgaccatcg cgcgcgtcca
     1621 ttccgcatcg gcaatcgtat agccgatgcg ccagcccgtc atcgcgtagg ttttcgaaaa
     1681 cgagccggca acacaaacaa aacgccgcaa ttcgtcgggc aaagcggcgg acgtgaaaac
     1741 ttccgacggc ggataagcga aaaacaggta acattcgtcc gtcaaaacat agacgttttt
     1801 ttcggcgcag gcttcgacga tttttcgcat ctcggcggcg gcaattacgc gaccggtcgg
     1861 attgttcggc gaattgacga ttaaaagcct ggttttgtcg gtaatggcgg ctttgacctg
     1921 ttcggcgttt aaaacaaaat cggtttcttc ggtttcgata aaaacgctct cggcgcggca
     1981 aaatttaacg atttcgggaa acgtcaccca gtagggc
//
LOCUS       DASWNE010000109         2072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_427634, whole genome shotgun sequence.
ACCESSION   DASWNE010000109 DASWNE010000000
VERSION     DASWNE010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427634"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1926)
                     /locus_tag="VGB68_02245"
     CDS             complement(<1..1926)
                     /locus_tag="VGB68_02245"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0048077.1"
                     /translation="MHILSTFKARRFAVAFLAFFVFAVCSVYGQAPTPTPFKDEIISV
                     RTDLIQTNVTVFDKNNRFVEGLKPEQFQLTIDGKPAKIEFFEAVAPLKTVENRENKST
                     GAVASRNLSLRGRKIVFFVDDLHLSLDSLGRTRSTINHFIENDMQPTDSVAIISASGQ
                     VGFLQQFTDNKAVLRAALARLRVLSDTFKDTDSPPMPPYTAVRIANGDRDAAGVYIQK
                     IVEGMKASGTGGSTSVNRNAVYETVKQRANNIVRGMTAVSESSLGSLENLLLTLNQAP
                     GRKIVYFISDGFYMQTERGQFIQNNLMQRAVNLATRSGSVIYTIDARGLFSAGMADAT
                     GERPVDFQSDSKSRLAEDTAAQEGLFVLANETGGRFLKNQNYFEKWVDRMLDENASYY
                     VLAWQPEKEESANKNFKRIEVGVVGRADLTVRLQRGYLANWEKSGEKIAGNKSGGKQT
                     ENSSFNISGDSSPKRFLPTNLSLNYLDVPNVGGVLTSSVQIDTDSLNYGDGGGSRQSA
                     AIDVVGLIYNEQGKQVADFKTGLSITPQTAATNAQSVIYNHRTPLAAGLYMVKVAARE
                     AKSGQVGIATQWIEIPDLSKRQLTLGSILLGAKNVKSNDATQQQVQFSVDHRFPRPFN
                     LNFMSFIYNAALASGGEP"
BASE COUNT          450 a          572 c          537 g          513 t
ORIGIN      
        1 cggttcgccg cccgaggcga gcgccgcatt gtatataaac gacataaaat tcagattaaa
       61 cgggcgcgga aagcgatggt cgacgctaaa ctgaacctgc tgctgcgtcg cgtcattgct
      121 ttttacgttt tttgcgccga gcagaatgct gccgagcgtc aattgccgtt tggataagtc
      181 aggaatctca atccactgcg tcgcaatgcc gacctgtccg cttttagctt cacgcgcggc
      241 gactttaacc atataaagtc cggcggcgag cggcgttcga tgattgtaga taacgctctg
      301 cgcgttggtc gcggcggttt gcggcgtaat gctcaagccg gttttaaaat cggcaacctg
      361 tttgccctgc tcgttgtaga taagcccgac gacgtcgatc gccgccgatt gcctgctgcc
      421 gccgccgtcg ccgtagttga gcgaatcggt gtcgatttgc acggaactgg tcagcacgcc
      481 gccgacgttc ggcacgtcca gataattcag agacagattg gtcggcaaaa accttttcgg
      541 gcttgaatcg ccggaaatgt tgaacgaaga attttccgtc tgtttaccgc cgcttttatt
      601 gcccgcgatt ttctcgccgc ttttttccca gttcgccaga taaccgcgct ggaggcggac
      661 ggtcaaatcg gcgcgcccga cgacgccgac ttcgattcgc ttaaaatttt tattcgccga
      721 ttcttccttt tccggctgcc acgccagcac gtaataactg gcgttttcgt cgagcatccg
      781 gtcaacccat ttctcgaaat aattctgatt tttcaggaaa cgaccgcccg tctcgttggc
      841 gagcacgaaa agcccttcct gcgcggcggt gtcttccgcc agtctcgatt tcgagtcgct
      901 ctgaaagtcg accgggcgtt cgcccgtcgc atccgccata ccggcggaaa acaatccgcg
      961 cgcgtcaatc gtgtaaataa cgctgcccga gcgcgtcgcc agattaaccg cccgctgcat
     1021 cagattgttc tggataaatt gcccgcgctc ggtctgcata taaaagccgt cggaaataaa
     1081 atagacgatt ttgcgtcccg gcgcctgatt cagcgtcaat aaaagatttt ccaatgaacc
     1141 gagcgaagat tccgaaacgg cggtcattcc acggacgata ttgttggcgc gctgcttgac
     1201 cgtctcgtat accgcgttgc gattaaccga agtgctgccg ccggtgccgc tcgccttcat
     1261 tccctctacg attttctgga tgtaaacccc ggcggcgtcg cggtcgccgt tggcgatgcg
     1321 gacggctgta taaggcggca tcggcggcga atcggtgtct ttaaacgtgt cggagagaac
     1381 tctcagacgc gccagcgccg cgcgcaaaac ggctttgttg tcggtaaatt gctggagaaa
     1441 gccgacttga ccgctcgcgg aaattatcgc cacgctgtcg gtcggctgca tatcgttttc
     1501 gatgaaatgg ttaatggtcg agcgcgtgcg tccgaggctg tccagcgaca gatgcaggtc
     1561 gtcaacgaaa aaaacgattt tgcgcccgcg caacgaaagg tttctcgagg caactgcgcc
     1621 ggttgattta ttttccctgt tctcaaccgt tttaagcggg gcgacggctt cgaaaaactc
     1681 gatcttcgcc ggcttgccgt ctatcgtcag ctgaaattgt tcgggcttca gaccctcgac
     1741 aaagcgattg tttttatcaa aaaccgtaac gtttgtctga atcaaatccg tgcggacgct
     1801 gatgatttca tctttaaaag gcgtcggcgt cggagcttgc ccgtaaacgg aacaaacggc
     1861 aaaaacaaaa aaagcgagaa aggcgacggc aaaccttctc gctttaaaag tacttaaaat
     1921 gtgcattatt actacctcga tgaaacttcg atgcgtgaat attaccttta tttaatcttc
     1981 agcgcaataa aattcgggta gtttccggct tcattccgcg cctaaaacct gtgcgaccgt
     2041 ttcttttttc aagcctttgc cgccgcccgt cc
//
LOCUS       DASWNE010000110         2441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_463209, whole genome shotgun sequence.
ACCESSION   DASWNE010000110 DASWNE010000000
VERSION     DASWNE010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463209"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /locus_tag="VGB68_02250"
     CDS             <1..1201
                     /locus_tag="VGB68_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048078.1"
                     /translation="DAQKRLEVENDLKSAHTIPCHHDAADAEFILKPVEKAVSPKVKP
                     EAKKTIPALHNEIPAERSKKSVKKQAEIKAEAKIEAPKPNKANQNSVENKVEADSKKI
                     SLMRKKRTVEKKPETVKSKNTAEPDKKVENAKIKTKAQKVEIQKVEIQKAEVSKANRA
                     PANTGETKIEKKPSGRKIEIPKPKKVNQTKTKIEKKPSEPKPVRALKKASVNQIKPKV
                     ESKAESSKLKSGAKLKSGAQKIAEAKAGKAKVSDAKTIEPPKKTAKKSEDAKTPKTVN
                     ALKTKIDNKAKIKIDNKAQTKIKISAESKTGQKTAKTKAGADSRTAKPAKIKAPETSK
                     ITKPAKVNQAGDTRAKAKGKTQAPKSEKPEKPKVKANTKAKISIKPTVKGKENRLKTS
                     SRKRLAF"
     gene            1272..1475
                     /gene="rpmE"
                     /locus_tag="VGB68_02255"
     CDS             1272..1475
                     /gene="rpmE"
                     /locus_tag="VGB68_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEY0048079.1"
                     /translation="MKAEIHPKYEEITVACACGNQFKTRSTMKDDLHIEICSECHPFF
                     TGKQKLVDTAGRVDRFNKRYARK"
     gene            1690..>2441
                     /locus_tag="VGB68_02260"
     CDS             1690..>2441
                     /locus_tag="VGB68_02260"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEY0048080.1"
                     /translation="MKKYLSLFLSIERDLIVGGQAVIEGVMMRTPSAYAIACRKSDGT
                     IVHTAEALPKWSDQYKFLNFPVIRGGATLIQSMALGVKALNFSANVFEEDAQAKEEKV
                     KAAKLKTEPVLAEGTTDENFLKAPATVTMPEKSKAKPKQAAGAVGSIIFALLFNILLF
                     IVAPLVLTNFLFIWLGWGEAPLIAAEASWWEIAKAYVWEIKPASWIAFNLIDGLVRML
                     FFVIMIFSMSFLKDIKRVFQYHGAEHKTVFNW"
BASE COUNT          844 a          547 c          579 g          471 t
ORIGIN      
        1 cgacgcgcag aaacgcctgg aagtggaaaa cgatttgaaa tccgcacaca cgattccgtg
       61 tcatcacgac gccgccgacg ccgaatttat attaaagccg gtcgaaaaag ccgtttctcc
      121 gaaagtaaaa ccggaagcga agaaaaccat tccggcgctg cacaatgaaa ttccggctga
      181 gcgttcgaaa aagtctgtaa aaaaacaagc cgaaataaaa gccgaagcaa aaatcgaagc
      241 tccaaaaccg aataaagcga atcagaattc cgtcgaaaat aaagtcgaag cagattccaa
      301 aaaaatcagc ttgatgcgga aaaagcggac ggtcgagaaa aaaccggaaa cggttaaatc
      361 gaaaaacacc gctgaaccgg ataaaaaggt tgagaatgcg aaaattaaaa cgaaggctca
      421 aaaagtagaa attcaaaaag tagaaattca aaaggcagag gtttcaaaag caaatcgggc
      481 tccggcaaat accggagaaa caaagattga gaaaaaaccg tccggtcgga aaattgaaat
      541 cccgaagccg aaaaaggtca atcaaacaaa gacaaagatt gagaaaaaac cgtcggagcc
      601 gaaacccgtc cgagctttga aaaaagcgag cgtcaatcaa attaagccga aagtcgaaag
      661 caaagccgaa tcttcaaaat taaagagcgg cgcaaaatta aagagcggcg cgcaaaaaat
      721 cgccgaggca aaagccggca aagcaaaagt cagcgatgca aaaacaatcg agccgccgaa
      781 aaaaaccgcg aaaaagtctg aagacgcaaa aacgccgaag acggtcaatg ccttaaaaac
      841 aaagattgat aataaagcta aaattaaaat cgataataaa gctcaaacta aaataaagat
      901 ttcagccgaa tcgaagaccg gacaaaaaac cgccaaaacc aaagcgggcg cggattctag
      961 aacggcgaaa ccggcaaaaa tcaaagcgcc cgaaacatcg aaaatcacca agcccgccaa
     1021 agtcaatcaa gccggagata cacgggcaaa ggcgaaagga aaaacgcagg ctccgaaatc
     1081 ggagaagccg gagaagccga aagtcaaagc gaacacaaaa gcgaaaatca gcatcaaacc
     1141 aaccgttaaa ggcaaggaaa atcgtttgaa gacctcatcg cgcaaacggc ttgcctttta
     1201 acgtattcaa aagtattatt aaggtttgcg ctgcggcgcg gattttgaat atagacaggg
     1261 taagaaataa aatgaaagct gaaattcatc cgaaatacga agaaattaca gtcgcttgcg
     1321 cgtgcggcaa tcaatttaaa acacgctcga cgatgaagga cgatttacac atcgaaatct
     1381 gctcggaatg ccatccgttt ttcacgggca aacaaaaatt agtcgatact gccggtcgcg
     1441 ttgaccgctt taacaaacgc tacgctcgca aataattatt tgatgcgcgt atttgcgata
     1501 cattactagc cactttaaaa tccgacgccg acgctccttt cacgcgccgt tgactcctgg
     1561 tgtaaaatgc cgacaggaaa acggaaagct gctgaaaaaa agcgcggcgt ccttttattt
     1621 ttattcggat ttgcgattcg gggttgatta gacatcttta gcagaaagtt tcaaatcaaa
     1681 ggaatgacta tgaaaaagta tttatcgtta tttttatcaa tcgagcgcga tttaatcgtc
     1741 ggcgggcaag ccgtcatcga aggcgtgatg atgcgcacgc cgagcgcgta cgccatcgcc
     1801 tgtcgcaaat ccgacggcac catcgtccac acggcggagg cgctgccgaa atggagcgac
     1861 caatacaaat ttttgaattt ccccgtaatt cgcggcggag cgacgctgat tcaatcgatg
     1921 gcgctcggcg tcaaggcgct gaatttttcg gcgaatgtgt ttgaagaaga cgctcaggcg
     1981 aaggaagaaa aagttaaagc cgccaagctg aaaaccgaac ccgttttagc cgaaggcacg
     2041 acggacgaaa attttctgaa agctccggcg acggtgacga tgcccgaaaa atccaaagca
     2101 aagccgaaac aggcggcggg agccgtcggc tcgataattt tcgcgctgct ttttaatatt
     2161 ctgcttttta tcgtcgcgcc gctggttttg acaaactttt tgtttatctg gctcggctgg
     2221 ggcgaagccc ctttgattgc cgccgaagcg agctggtggg aaatcgccaa ggcttacgtc
     2281 tgggaaatca agcccgcttc gtggattgcg tttaacctga ttgacgggct cgtccggatg
     2341 ctttttttcg tgataatgat tttttcgatg tcgtttttaa aagacatcaa acgcgttttt
     2401 caatatcacg gcgcggagca caaaacggtt ttcaactggg a
//
LOCUS       DASWNE010000111         1883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_251101, whole genome shotgun sequence.
ACCESSION   DASWNE010000111 DASWNE010000000
VERSION     DASWNE010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251101"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VGB68_02265"
     CDS             <1..384
                     /locus_tag="VGB68_02265"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0048081.1"
                     /translation="ISTNPTNQPNSVKAVNFGLSSDAAIVEDYDNDGKTDVAVYRAGA
                     LSYWYILRSSDNQFQEIQFGASGDKPQPGDYNGDGKTDVAVFRPSTGSWYIAKPTGVP
                     AQNFYSIQWGAATDIPVSSMTTISQ"
     gene            573..1256
                     /locus_tag="VGB68_02270"
     CDS             573..1256
                     /locus_tag="VGB68_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0048082.1"
                     /translation="MLQNTVNNFIETKRMQIPEIIKVAIIEDRREIREGLAMLINATD
                     GYQCPATYRSMEEAIGKIKYNLPDVVLSDIGLPGMDGIEGIKILKEQFPDLTILMLTV
                     YDDNERIFDALCAGACGYLLKKTPPAKLIESLREAVGGGSPMSPEVARRVITLFRNFR
                     PPEKADYDLTPHEARLLKLLVEGHSYKSAALELKVSVNTIAFHLKNIYENCRFIRNRK
                     PSPKLCRIA"
     gene            complement(1355..>1883)
                     /locus_tag="VGB68_02275"
     CDS             complement(1355..>1883)
                     /locus_tag="VGB68_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048083.1"
                     /translation="FVLGVSTLFYNYARFHSFTDFGYARIPGVLDEPWYNHGIFSIWY
                     IPRQAYEMLFKLWESKAAFPYLVPNGFSSSILWSSPFILLALRFGARDKILKYAAWTA
                     IAVLTILLWMHGNSGGWQFGYRYWMVALPWLFVVLLENSPEEITPLEWITYIISFILN
                     AYATYLFFWTDYVKP"
BASE COUNT          522 a          465 c          478 g          418 t
ORIGIN      
        1 atttcgacta atccgaccaa ccagccgaac agcgtcaaag cagtcaattt cggcttaagt
       61 tccgacgcgg caatcgtcga agattacgac aacgacggta aaacggacgt cgcggtttat
      121 cgcgccggag ctttgagcta ctggtacatc ctgcgcagca gcgacaatca atttcaggaa
      181 attcaattcg gcgcgagcgg cgacaagccg cagccgggcg attacaacgg cgacggcaaa
      241 acggacgtcg cggtgtttcg cccgtcaacc ggaagctggt atatcgccaa gccgaccggc
      301 gttccggcac agaatttcta ctcgattcaa tggggcgcgg caaccgacat cccggtttcg
      361 tcgatgacga cgatttcgca gtaaccgttt tcgaagcgcg gcttcgatgg atagcggaag
      421 attaaaaatc cgctatcctt tttttatttt tacgggtaga ttattgaaac ggcggctcgg
      481 attacggcga aaacgttact actgaaatat gtggcgcgct tatctctgaa aaaccgctaa
      541 gcttctacct gttcgagagg agaggtttta taatgctgca aaataccgtt aataatttta
      601 tcgaaactaa acgaatgcaa attcccgaaa ttatcaaagt cgccattatc gaagaccgcc
      661 gcgaaatccg cgaaggcttg gcgatgctta tcaacgccac cgacggctat caatgtccgg
      721 cgacgtatcg ctcgatggaa gaagccatag gcaaaataaa atacaatctg cccgacgtgg
      781 ttttgtccga catcggcttg cccggaatgg acggcatcga aggcatcaaa atcttaaaag
      841 agcagtttcc ggatttgacg attctgatgc tgacggttta tgacgacaac gagcgaattt
      901 tcgacgcttt gtgcgcgggc gcgtgcggct atcttttgaa aaaaacgccg cccgccaaat
      961 tgattgaatc cctgcgcgaa gccgtcggcg gcggctcgcc gatgtcgccc gaagtggcgc
     1021 ggcgcgtgat aaccttgttt cgaaattttc gcccgccgga aaaggcggat tatgatttga
     1081 cgccgcacga agctcggctg ttgaagcttc tggtcgaagg tcacagctac aaatcggcgg
     1141 cgctggaatt aaaggtgagc gtcaatacaa tcgcctttca cctgaaaaat atctacgaaa
     1201 attgcaggtt cattcgaaat cggaagccgt cgccaaagct ttgcagaatc gcctgattta
     1261 attaaacgat tctctacaat tacgaattac gaattacgaa tgaagaaaag attcttttaa
     1321 ttcgtaattc gtaattcgta attgatttac gcctttacgg cttgacgtaa tccgtccaga
     1381 agaaaagata ggtcgcgtaa gcattgagga taaaggagat gatataagta atccattcga
     1441 gcggcgtgat ttcttcgggg ctgttttcga gcagaaccac gaacagccac ggcagagcca
     1501 ccatccagta gcgataaccg aattgccagc cgccggaatt tccgtgcatc cagagcaaaa
     1561 tcgtcagcac ggcgattgcc gtccaggcgg catatttcag gattttatcg cgcgcgccga
     1621 accggagcgc gagcagtata aacgggctgc tccaaagaat cgaactgctg aaaccgttcg
     1681 gcaccagata aggaaacgcc gctttgcttt cccagagctt gaaaagcatc tcgtaagcct
     1741 gacgcggaat ataccaaatc gaaaaaattc cgtgattgta ccagggctcg tccaaaacgc
     1801 ccggaattcg cgcgtagccg aaatcggtga acgagtgaaa acgcgcgtaa ttgtaaaaca
     1861 gagtggagac gcccagaacg aac
//
LOCUS       DASWNE010000112         9412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_3733, whole genome shotgun sequence.
ACCESSION   DASWNE010000112 DASWNE010000000
VERSION     DASWNE010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9412
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3733"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(378..857)
                     /locus_tag="VGB68_02280"
     CDS             complement(378..857)
                     /locus_tag="VGB68_02280"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY0048084.1"
                     /translation="MTAKKSASFASRFLWKAGFPICARKPSRKADVSRLLFGASYFHY
                     REGVYTGKLLVLIDRRTASSAEAFAAMLGDSGAAAIVGQPSIGAGCGYTNGGIEIVLK
                     NSGGRVKMPDCVRFRADGSNEAAGITPDISIPWRQNDTAFQKAKQTLEMLEKISTSK"
     gene            848..970
                     /locus_tag="VGB68_02285"
     CDS             848..970
                     /locus_tag="VGB68_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048085.1"
                     /translation="MPSLRFMTPAGRYFELSFSSARKFFKAEKATPGLAFGFGR"
     gene            1244..1672
                     /locus_tag="VGB68_02290"
     CDS             1244..1672
                     /locus_tag="VGB68_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048086.1"
                     /translation="MLHLSIIRQHFDAGFSSVLSLIEELEQQIESLTPAHQSSSHIQH
                     LEQTIAHQKSEISRLSKTVENKSKEIFKLHQTHHHLQDKLQIRLSKANQLNRAGFANA
                     QIAAENIRLFSLDGGRSRVLSGALVFIVGAKARTSFAYCS"
     gene            1834..2586
                     /locus_tag="VGB68_02295"
     CDS             1834..2586
                     /locus_tag="VGB68_02295"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0048087.1"
                     /translation="MSYIYLEHSPSPRLDSVVASYWSLQVSEGEEPGNHRVLPDGCVD
                     LLFDNISRPSGVIIGSMTRAVSVTLQPNAKYFGVRFRPGGASSFLDISLKEIADTQIK
                     LKDVWRNAHSEVSHVMGNKQVKNQIETVENFLEKQFSDHAAGSPKVQAAIALLNSHDG
                     NYPVNMLSKTLDISRQHLNRIFTEAVGLNIKMFSRIVRLKAALKRARRAPVKDWALFA
                     LESGYYDQAHFISDFKELTGTTPTNFFGRKIR"
     gene            2731..3075
                     /locus_tag="VGB68_02300"
     CDS             2731..3075
                     /locus_tag="VGB68_02300"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEY0048088.1"
                     /translation="MENQLVAAAESFALELEFKPMFGGVCAYVKSRIFASLSDVGLAL
                     KLSPTAQTELLKLEEAKRLQYETDSTPSKQYIVVPPEVWTDERDLAVWLKQSVDFVMA
                     QPAPKSKRKKTP"
     gene            3429..3824
                     /locus_tag="VGB68_02305"
     CDS             3429..3824
                     /locus_tag="VGB68_02305"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0048089.1"
                     /translation="METSKANTHFHGLRTIVYYAPDLDKAKSWYSSVLGIEPYFDQPF
                     YVGFNVGGYELALDPDASSTPGGNAGAVTYWGVADAQTAFDRLISLGAIERSGVQDVG
                     DGIRVATVFDPFGNIFGIIENPYFNLPNE"
     gene            complement(4189..4701)
                     /locus_tag="VGB68_02310"
     CDS             complement(4189..4701)
                     /locus_tag="VGB68_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048090.1"
                     /translation="MKYEIFERANKHFTCFNSLDDSRFGAAAGGADFQRDAMVRRFCP
                     AKAGNVSRLRQSRMGNRRTRAGFETAFGRNRIELAKSARPGGSAAGNRNISARNYSGS
                     FRESSHLKKCENRNKYSINFILYIRCKSVFYFNNFLKPDQSIPQAKQGEFWDFGYDRT
                     FDTSRKVHKP"
     gene            4876..6036
                     /locus_tag="VGB68_02315"
     CDS             4876..6036
                     /locus_tag="VGB68_02315"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY0048091.1"
                     /translation="MNPIQMVDLKGQYEKIKAEIDEAVLACVASTAYINGKEVQSFQK
                     NLENYLGVRHVIPCANGTDALQIAMMALGLKEGDEVIVPSFTYVATAEVIALLKLQPV
                     MVEVDADTFNITAEIIEKAITPKTKAVVPVHLFGQACDMEAIMRVAEKHNLFVIEDNA
                     QAIGADYTFADGSRKKAGTIGHIGTTSFFPSKNLGCFGDGGAIFTNDDNLAELMRMIA
                     NHGQTRRYYHSYIGVNSRLDTLQAAILDVKLKRLDAYCDARRKAADFYDAAFAEIAEL
                     QTPVRQRNSTHVFHQYTLQIKDGRRDALMAHLQEKGVPSMTYYPLPLYRQIAFAPFVP
                     PDFVLPITEKLCDSVLSLPIHTEMSEELLDYIAARVKDFFANRQEYGKMQSV"
     gene            6100..6231
                     /locus_tag="VGB68_02320"
     CDS             6100..6231
                     /locus_tag="VGB68_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048092.1"
                     /translation="MVKGGDTTKAYCSKCMRVTAWTYVAGTLSSYWKCLACGMNRPA"
     gene            6402..7316
                     /locus_tag="VGB68_02325"
     CDS             6402..7316
                     /locus_tag="VGB68_02325"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0048093.1"
                     /translation="MISTSTENEPVNNSNPVSGNSLGVIARRHELPEKPLVVIEPSRN
                     LVALNLRDLWHYRDLFYILTMRDIKVRYKQTFLGVAWAIIQPLFTMIIFTLLFGRLAN
                     MPSDGVPYPLFAFAGLLPWTFFSNAVTNSGNSLVGSSNLITKVYFPRMVIPFAAVAAG
                     LLDLIVAFGLMIILMFYYGVGFSVNMLMLPVLTVVLTLLAVGIGMFLSALNVKYRDIR
                     YALPFLIQLGMFASPIIYPLSIIPEKWRWLMMLNPLSGLIEGFRAALFGKSFDWTSLG
                     VSLAMTLAILCFSAYVFRKMERSFADVI"
     gene            7319..8572
                     /locus_tag="VGB68_02330"
     CDS             7319..8572
                     /locus_tag="VGB68_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0048094.1"
                     /translation="MKPIIKVENLSKRYTLGGAQTPYSTLRESLVQTAKAPLNWVRRA
                     GRASEGETLWALKDVNFEVMPGEVVGVIGRNGAGKSTLLKVLSRITEPTQGKIELYGR
                     IGSLLEVGTGFHPELTGRENIFLNGAILGMRQTEIAKKFDEIVDFAEIEKFIDTPVKF
                     YSSGMYTRLAFAVAANLEPEVLIVDEVLAVGDAEFQKKCLGKMKSVSDKEGRTVLFVS
                     HNMIAVKNLCQRALYMQKGELTFDSDANSVINHYLQNGVEMASQRAWADAEMPGNEKI
                     KVRRVAVYAENKLPEEPILTSDNVVIETEFYNNLDGAKIDVTWDLTDNQGIHVAHIGT
                     VCMNGEEMKKGFYRTTGVFPGNVLNSKKYILSIMFGQNQRVPLLRMEETLVFDVEDNL
                     RTRDSNFSQFPGVIHPICDWRTAAL"
     gene            8616..9353
                     /locus_tag="VGB68_02335"
     CDS             8616..9353
                     /locus_tag="VGB68_02335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEY0048095.1"
                     /translation="MSGQYKRDEEGRDLVIVGVGETAELAYEYFKFDSPHDVVGFAVE
                     AAYKDRDEFFGLPVVAFEEIEKHFSPAEHLAFAAISYTQLNRVRARIFKTLKAKGYTC
                     ASMISSAAFVWHNAEIGENCFIFENNVIQYKVKIGDNVVLWSGNHIGHQTVIQDNVFI
                     SSHVVVSGYCEVGANSFLGVNSSVANDVRIAPDNIIAMGAVIHKHTEPRKIYKGNPGK
                     PDETKDSFAVMKVKEETETATGENANR"
BASE COUNT         2434 a         2221 c         2337 g         2420 t
ORIGIN      
        1 ggcgagattt atcacggcaa caaacctgaa ggcgcgataa gcgaacatta attttctgta
       61 ataaaggcgg aatcgctacc gcctttatta tcaatcgaaa ctattaattt caaacaaatc
      121 aaagtagttt tcaatcacga agacattagg actaatcggc ggcatcggtt ggttttccgc
      181 ttcgatttat tacaaaacga ttaaccggct tgtcaattaa cggcgcggcg cggcgcattc
      241 ggctaaactt ctgttgaaac tttacggctc tctctgtgtg tgtgccgagc tactttttcg
      301 cctgcaattt gcgccgggac taaagatata gagctgccgg tcgaacgggc aaactaaata
      361 ttgcgcgggt tgcctgatta ttttgacgtc gatatttttt caagcatttc aagcgtctgt
      421 tttgctttct gaaaggcggt gtcattctgc cgccacggaa ttgaaatatc cggcgtgatt
      481 ccggcggctt cgtttgagcc gtcggcgcga aaacgcacgc aatccggcat tttaacgcgc
      541 ccgccgctgt ttttaagaac gatttcgatt ccgccgttcg tatatccgca gcccgcgcct
      601 atcgaaggct gcccgacgat tgccgccgcg ccgctgtcgc cgagcatcgc cgcaaacgct
      661 tccgccgacg aagccgtccg ccggtcaatc agcaccagca gctttcccgt gtagacgcct
      721 tcgcggtagt ggaaatagct cgcgccgaaa agcaggcggc tgacatccgc tttccgcgac
      781 ggcttgcgcg cacaaatcgg gaaacccgct ttccataaaa agcgcgatgc gaaggacgcc
      841 gatttttttg ccgtcattga gatttatgac gcccgcgggc agatatttcg agctttcatt
      901 ttcgagcgcg cgaaagtttt tcaaagccga aaaagccacg cccggcttgg cgttcggatt
      961 cgggcgatag ccgagccgct cgcaaagacg gctcggcgcg gcttcggaat taagcggcga
     1021 ttcgtctgat ttgccgttcg tcgtccattc gattctcaga tgcgcgtcgg cgaaccggcg
     1081 cagaagtaac tgttcaggtg cttcataagc aaactatttt gagtagtttg cgcatactga
     1141 ttttttatca ttcaactcta cacaaaaata atttcttgct tttgtttcca ggttttcgta
     1201 tatttatttc atcctgtaat cgccatcgtt gaggtcatag tcattgctcc atctttcaat
     1261 cattcgtcag cattttgatg cgggttttag ttccgtcttg agcctgatcg aagaactcga
     1321 acagcagatt gaatctttga ctccggctca tcaatcttca tcccacattc agcatttgga
     1381 gcagaccatt gctcatcaaa aatcggaaat cagtcgtttg agcaaaaccg ttgaaaacaa
     1441 atcaaaagaa attttcaaac ttcaccaaac tcatcaccac ttacaggaca aattacaaat
     1501 tcgattgtcg aaagccaatc aattgaaccg agcgggattt gcgaatgctc aaattgcagc
     1561 agaaaatatc cgtctgtttt cgctcgacgg cgggcgcagc cgtgttctgt cgggtgcgct
     1621 cgtatttatc gtcggcgcga aagcaaggac aagctttgct tactgctctt gaatccgctt
     1681 taaaaggtaa gccaatcgga ttgattatct agtaataatt gaaattgctg atttattatg
     1741 gtgtttattg acaaaaacca ccgtttcctc acttttccga accggcgaat ttaaccggct
     1801 ggcgaatgga tttttggtat gaaaacgcaa aaaatgtctt atatttacct cgaacacagt
     1861 ccgagtcccc ggctagattc agtcgtggct agttattggt cattgcaggt ttctgaaggc
     1921 gaagagccgg gaaaccatcg agttttaccg gacggctgcg tcgatctgtt atttgataat
     1981 atctcgcgac cttcaggcgt gattatcggc agtatgacca gagcggtgtc ggtaactttg
     2041 caaccgaacg ccaaatattt cggcgttcgg tttcgtcccg gcggcgcgtc ctctttctta
     2101 gacatctctt tgaaagaaat agccgatact caaataaaat taaaagatgt ctggcgaaat
     2161 gctcattcgg aagtttcaca cgtaatgggt aataaacaag tgaaaaatca aatcgaaacg
     2221 gtcgagaatt ttttagaaaa gcaattttcc gatcacgctg ccggaagccc gaaagtccaa
     2281 gccgccatcg ccctgcttaa ctcgcacgac ggaaattacc cggtaaatat gcttagtaaa
     2341 acgctcgaca tcagccgcca acatcttaac cgaattttta ccgaagcggt cggacttaat
     2401 attaaaatgt tttcccgtat cgttcgcctt aaggctgccc ttaagcgcgc ccgccgcgcg
     2461 ccggtcaagg attgggcgtt atttgctctg gaaagcggct attacgacca agctcatttt
     2521 atttccgact ttaaagaatt gacgggaacg acgccaacca attttttcgg acgtaaaatt
     2581 cgatgaagtt tcctatcctc cagcgttcca tttttccaat acgaacccgc ctgataatta
     2641 gtagaatcga aaactatttg aagattacga ctaattatgt tgaataagag aatgagtaat
     2701 aagcaatcgg aaaagaagca attagcggag atggaaaatc agcttgtcgc ggcggctgaa
     2761 agtttcgcat tggaactgga atttaagccg atgttcggcg gtgtttgcgc ttatgttaaa
     2821 agccgcatat ttgcatcgtt gtcagatgtc ggactggcac ttaaactttc gccgaccgcg
     2881 caaacggaat tattaaagct ggaagaagcg aaacggctgc aatatgaaac cgactcgact
     2941 ccgagcaagc aatatatcgt tgtgccgccc gaagtctgga cggacgagag ggatttggca
     3001 gtttggctca aacaaagtgt agattttgtg atggcgcaac ccgcgcccaa atcaaagcgc
     3061 aaaaaaactc cgtaaaacat caattttata cgggttgaat ttataaagga tttagaagtt
     3121 gagagaatca ttatgacatc aatcaaaaat caggtccgat gatattgccg ccgtcgtcgc
     3181 tttgtctgaa gcgagcggat tgttcaattt cggacggaat cgagttaata agagaaaggc
     3241 tgtccgatta tgagggcggt aatgacgaca tttggtttgc cgccgtaaat gaaaaaatac
     3301 agagagcgat atattgcatc cgcgaaccaa tgacggacgg aatacggaat atcttaatgc
     3361 tgattgaaat tcagacgatg aggtaattct gtcattagaa ttggaataga acggttaaaa
     3421 taagttatat ggaaacatca aaagcaaaca cacattttca cggtcttcgc accatcgttt
     3481 attacgcgcc agacctcgac aaagccaagt cttggtattc gtcagttctc ggtattgagc
     3541 cgtactttga ccaacccttt tacgtcggct tcaatgtcgg cggttacgaa ttggctctcg
     3601 accctgacgc ttccagcaca ccaggcggta atgccggtgc tgttacatat tggggcgttg
     3661 cggatgcaca aacggcgttt gaccgtctta tttcactcgg ggctattgag cgttcaggag
     3721 tgcaagacgt cggcgacggt attcgagttg ctactgtttt cgacccgttc ggcaacatct
     3781 tcggcatcat tgaaaatccg tatttcaatt tgccgaatga ataaaagcaa tgttttgtat
     3841 tgggcggtca gccgaacaat tcaatggata tgagggcgaa acagtgactt tgttatttgg
     3901 cttgtccgtt aaactttagc gagcttaaca gcagaaaatg ccgaatgcgc tgcgcagacg
     3961 atagccggat tgacgaaacg gtagtcactc acgacaaaac cgggatgttt tacttcggct
     4021 tgcgcgtcgc ggaaatgaaa ctgacgatta gagttgagcg ataatgctgt cgaacaaatc
     4081 agtggacgcg agggcgcagg cagcgctttc tctcaatttc aaaaacgtcc caaaacggtg
     4141 tcataagtag cattttttga gacagtttaa ttactgaatt gcttcatttt aaggcttatg
     4201 aaccttccgc gatgtgtcaa aagtccggtc ataaccaaaa tcccaaaact ccccctgttt
     4261 agcttgtggg atagattgat ccggctttaa aaaattattg aaataaaaaa ccgatttaca
     4321 tcgaatataa agaataaaat tgatgctgta tttatttctg ttttcgcatt ttttgaggtg
     4381 ggaactttca cgaaagctac ctgaatagtt acgcgcagaa atatttcgat tgcctgccgc
     4441 gcttccgccc ggtctcgcgc ttttcgcgag ctcgattcgg tttcgtccga aagctgtttc
     4501 aaatccagcc cgcgttcttc gattgcccat tcgagattga cgtaaccggc tgacatttcc
     4561 cgctttagcc gggcaaaatc ttctaaccat tgcgtcgcgc tgaaaatctg ctccgccggc
     4621 ggctgcgccg aaacgggaat cgtccagaga attaaaacaa gtaaaatgct tattggcgcg
     4681 ctcgaaaatt tcatatttca aaatccttaa aatcgtatga atacacgccg tcagactaat
     4741 atttgttctc tttcggcggc ggcggcgaaa actctcgttt tggcaaattt cgctgaaacc
     4801 gttatgattt taatttgcat ttccccggtc ggctgtaacg gttatttatt gttttatatc
     4861 aaaagtcggt aatttatgaa tcctattcag atggttgacc tgaaaggtca atacgaaaaa
     4921 atcaaagccg aaatcgacga agccgttctg gcttgcgtcg cttcgaccgc gtatattaac
     4981 gggaaagagg ttcagtcttt tcagaaaaat ctggaaaact atctcggcgt ccgtcacgtc
     5041 attccctgcg ccaacggcac ggacgctttg cagattgcga tgatggcgct cggtttgaag
     5101 gaaggcgacg aggttatcgt gccgagcttt acatacgtcg ccaccgccga agtcatcgct
     5161 ttgctgaaac tgcagcccgt gatggtcgaa gtcgacgccg atacgtttaa catcacggcg
     5221 gaaatcatcg aaaaagcgat tacgccgaaa acgaaagccg tcgttccggt tcatcttttc
     5281 gggcaagcct gcgatatgga agcgattatg cgggtggcgg aaaaacataa cctgttcgtt
     5341 atcgaagaca acgcgcaggc gatcggcgcc gattatactt tcgccgacgg aagccgaaaa
     5401 aaagccggaa ccatcggtca catcggcacg acatcgtttt tcccttcgaa aaacttaggc
     5461 tgtttcggcg acggcggcgc gatttttacg aacgacgaca atctggcgga attgatgcgg
     5521 atgattgcca atcacggtca gacgcggcgc tattatcatt catatatcgg cgtcaattcg
     5581 cggctcgaca cgcttcaggc ggcgattctc gacgttaaat taaaacgtct ggacgcgtac
     5641 tgcgacgctc gcagaaaagc cgccgatttt tacgacgcgg cttttgccga aatcgcggag
     5701 ctgcaaacgc cggttcgcca gcgcaattcg acgcacgttt ttcatcaata cactttgcaa
     5761 atcaaagacg gtcggcgcga cgcgctgatg gcgcatttac aggaaaaagg cgtgccgtcg
     5821 atgacttatt atcctctgcc gctttatcgc caaatcgctt ttgcgccctt tgtgccgccg
     5881 gatttcgttt tgccgatcac ggaaaagctt tgcgattcgg tgctctcgct cccgattcat
     5941 accgagatga gcgaggaact gctcgattat attgccgcgc gggtgaagga tttttttgca
     6001 aaccggcagg aatatggcaa aatgcagagt gtttgagaat ttttcacttg tgttaattat
     6061 gccggcaagt caggacaaag gcggcaggag ccaggaacta tggtaaaggg cggagataca
     6121 actaaagcct attgttcgaa atgtatgcgg gtgactgcct ggacatatgt cgccggaacg
     6181 ctttcctctt attggaaatg tctggcgtgc gggatgaatc gtccggcgta atatcagata
     6241 agccgccgcc attattacga gtgaaaataa aagctaccgg ctaaagacag acagccgttg
     6301 ccgagaaaat ttaaagcccc cgtttgattt atactaattc aataactaca cttgagtttt
     6361 gcaattttag ctttactcac cgaatgagtt aataacttac gatgatttct accagcactg
     6421 aaaacgagcc ggttaacaat agcaatccgg tttcgggaaa ttctttagga gttattgctc
     6481 gacgccacga actgcccgaa aagcctctcg tcgtcatcga accgagccgc aatctggtcg
     6541 cgctgaattt gcgcgatttg tggcattacc gcgatttgtt ctacatcctg acgatgcgcg
     6601 acatcaaggt tcgctacaag caaacctttt taggcgtcgc gtgggcgatt atccagccgc
     6661 tttttacgat gataattttc acgctgcttt tcggaaggct ggcaaatatg ccttcggacg
     6721 gcgtgccgta cccgcttttc gcctttgccg gattgctgcc gtggacgttt ttctcgaacg
     6781 ccgtgaccaa cagcggaaac tctctggtcg gcagctcgaa tctgattacg aaagtttatt
     6841 ttccgcggat ggtgattccg tttgccgccg tcgccgccgg gcttctggat ttaatcgtcg
     6901 ctttcggctt gatgattata ttaatgtttt attacggcgt cggcttttcg gtaaatatgc
     6961 tgatgctgcc ggttttgacc gtcgtgctga cgctgctcgc cgtcggcatc gggatgtttc
     7021 tgtccgcgct caacgtcaaa tatcgcgata ttcgctacgc tctgccgttt ctgattcagc
     7081 tcggaatgtt tgcctcgccg attatttacc cgctcagcat tattccggaa aaatggcgct
     7141 ggctgatgat gctcaatccg ctttccggct tgattgaagg ctttcgcgcg gcgcttttcg
     7201 gaaagtcttt cgattggacg agcctcggcg tctcgctcgc tatgacgctg gcgatattgt
     7261 gtttttccgc ttatgttttc cgaaaaatgg agcgcagctt tgccgacgtg atttaaaaat
     7321 gaagccgatt atcaaagtcg aaaatttaag caaacgctac acgctcggcg gcgcgcagac
     7381 gccgtattcc acgctcagag aatcgctcgt gcagacggcg aaagcgccgc tcaactgggt
     7441 caggcgcgcc ggcagagcga gcgagggcga aacgctttgg gcgctgaaag acgttaattt
     7501 tgaagtaatg ccgggcgaag tcgtcggcgt catcgggcgc aacggcgcgg gcaaatccac
     7561 gcttttaaaa gtcctttcgc gcattaccga accgacgcag ggaaaaattg aattatacgg
     7621 tcgcatcggt tcgctgctgg aagtcgggac gggttttcat cccgaactga cgggcagaga
     7681 aaatatcttt ttaaacggcg ctatcttggg aatgcggcaa accgaaatcg ctaaaaagtt
     7741 tgacgaaatc gtcgattttg ccgagattga aaaatttatc gacacgcccg ttaaatttta
     7801 ttccagcggt atgtacacgc ggctggcgtt tgccgtcgcc gccaatctgg agccggaagt
     7861 tttgattgtc gatgaagttt tagccgtcgg cgacgccgag tttcagaaaa aatgtctggg
     7921 caaaatgaag tccgtctccg acaaggaagg cagaacggtt ttgttcgtct cgcataatat
     7981 gattgcggtc aaaaacttgt gccagcgcgc cttgtatatg cagaagggcg agctgacttt
     8041 tgattccgac gccaattccg tcatcaatca ttatctgcag aacggcgtcg agatggcttc
     8101 gcagcgcgcg tgggcggatg cggaaatgcc cggcaacgag aagataaagg tgcgccgcgt
     8161 cgccgtctac gcggaaaaca aattgccgga agagccgatt ttaacgagcg ataacgtcgt
     8221 tatcgagacc gagttttaca ataatctgga cggcgcgaaa attgacgtga cgtgggattt
     8281 gaccgacaat caaggcattc acgtcgcgca tatcggcacg gtttgtatga acggcgaaga
     8341 gatgaaaaag ggtttttaca gaacgaccgg cgtttttccg ggaaacgttc tgaactcgaa
     8401 aaaatatatt ctgagcataa tgttcggtca aaaccagcgc gtgccgcttt tgcggatgga
     8461 agagacttta gtgttcgacg tggaagacaa tctgcgcacg cgcgacagca atttcagcca
     8521 gtttccgggc gtcattcacc cgatttgcga ctggcggacg gcggcgctct aaataaaaaa
     8581 gtaataatta ttcattgatg taacgaggtg acattttgtc aggacagtat aaaagagacg
     8641 aggaaggaag ggatttagtc atcgtcggcg tcggcgagac cgccgagctc gcctacgagt
     8701 atttcaagtt tgattcgccg cacgacgtag tcggcttcgc cgtcgaagcg gcttacaaag
     8761 accgcgacga gtttttcggc ttgccggtcg tcgcctttga ggaaatcgag aaacatttca
     8821 gccccgcgga acatctggcg tttgccgcga tttcctatac gcagctgaat cgcgtgcggg
     8881 cgagaatatt taaaacgctg aaagccaagg gctacacctg cgcgtctatg atcagcagcg
     8941 ccgctttcgt ctggcacaac gcggaaatcg gcgaaaactg ttttattttt gaaaacaacg
     9001 tcattcaata caaggtaaaa atcggcgaca acgtcgtttt gtggagcggc aatcacatcg
     9061 ggcatcagac cgtcattcag gacaatgtct ttatctcgtc gcacgtcgtc gtctccggct
     9121 attgcgaagt cggcgcaaac tcgtttctgg gcgtcaacag ctcggtggca aacgacgttc
     9181 gcattgcgcc cgacaatatt atcgcgatgg gcgcggtgat acacaaacac accgagccgc
     9241 gcaaaatcta caaaggaaat cccgggaagc cggacgaaac gaaagacagc tttgcggtga
     9301 tgaaagtcaa agaagaaacc gaaacggcaa cgggagaaaa cgcgaaccga tgaagtggaa
     9361 taaacaaggc ttgatttacg cgccggacgg aagtttggat tgggcaaaaa aa
//
LOCUS       DASWNE010000113         2469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_39204, whole genome shotgun sequence.
ACCESSION   DASWNE010000113 DASWNE010000000
VERSION     DASWNE010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39204"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(209..679)
                     /locus_tag="VGB68_02340"
     CDS             complement(209..679)
                     /locus_tag="VGB68_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048096.1"
                     /translation="MKKIILFSLLVFLLQSSFVFGQTAPNRYEAELLMNPNPGRKDTR
                     EINAVIVFEKDSVKIYLRRKNVVFKEFDYKDIKFAEHSFSKNPLASGQTQTLLLMMLT
                     GVPMLYGGKEKHWLTIVGEGDFAVLKIETDNYRLLKMEFIIRNLDVRNINEDRQ"
     gene            complement(856..1524)
                     /locus_tag="VGB68_02345"
     CDS             complement(856..1524)
                     /locus_tag="VGB68_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048097.1"
                     /translation="MKSISTILLLMIFASTVFAQTETLTNTEIVEMTRAGLGRQIILQ
                     KIASADGNFDISTKALIELKKAGVDDEVIQGLIEKNRATPVNVAGGQTPSFSESQPAT
                     PAENKAFIENKAVTPAEALRSAKTIALVKSSLQPSRQALEKELMKRADWKKLNLTLVR
                     YRESADLYVEIGYVSLSWITHRYVFRVYDRRSGAVIAAGETTSWGSLPANLAREISKK
                     LNSI"
     gene            complement(1531..2019)
                     /locus_tag="VGB68_02350"
     CDS             complement(1531..2019)
                     /locus_tag="VGB68_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048098.1"
                     /translation="MKKITLFLIVVFLSNFLTSVKAQSRAADVSFNEIKLLVQDGNNA
                     GEKSVRLRLDRESLTIASKNGAITKTFLYSDIRSAEYSYSKNPRWKTGLGLGAAGVLF
                     PPLWFVALPVAFTKHRRHWLTIRTGDDYAVLKLSKSSRKMLIPAFETKSGVSIRGVGE
                     EK"
     gene            complement(2116..>2469)
                     /locus_tag="VGB68_02355"
     CDS             complement(2116..>2469)
                     /locus_tag="VGB68_02355"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048099.1"
                     /translation="AGGMLDVYFAMRFLQLRDNVPDDAENRSTRAMLRKLRENGSLDE
                     ADFQAFDDGYDFLSCLDHSLRLTVGRTNRLPMANPSAMRTIVERMNLASTNDLLEKLT
                     FHRLEIRGAFENALK"
BASE COUNT          625 a          600 c          581 g          663 t
ORIGIN      
        1 gatagtagcc gatatgaaaa cacacggctt ggatgttaaa gaagttcagg aagaagacaa
       61 aaaaggtaaa taagcctttg aaaactcgag cctgagctga tttatttacg ccggctttta
      121 taagacaaag ccgcatcgaa atattttgcg cagcgcctct gaaatttaaa agcgaatttg
      181 gtaagccgcg agccgaccaa attcgctttt attgcctgtc ctcgtttata ttccggacgt
      241 ccagatttct gatgataaat tccattttaa gcaaacggta attgtcggtc tctattttga
      301 gcacggcgaa atcgccttcg ccgacaatcg tcagccaatg tttttccttg ccgccgtaga
      361 gcatcggcac gcccgtcagc atcatcaaaa gcagcgtctg cgtctgtccg gaagccagcg
      421 gattttttga gaaagaatgc tcggcaaact tgatgtcttt ataatcaaac tctttaaaaa
      481 ccacgttttt ccggcgcaaa tatattttta ccgaatcttt ctcgaaaacg attacggcgt
      541 tgatttcgcg cgtatctttt ctgcccggat taggattcat caacaattcc gcctcgtatc
      601 gattcggcgc agtttgaccg aacacgaaag acgattgcag cagaaaaacg agcagcgaaa
      661 acaaaattat ctttttcata attcgtattt aacccggtga caatcggaaa acggaaaagc
      721 accgtcaaag cgacggcttt aactctaaat tttaaattct ttcggaaaaa aataatgcaa
      781 atcagtttaa attttaaaat ttagaaatcc gccgcgcgtt ttccgggcgt tttgaatttc
      841 cttaatttat tcaatctaaa tcgagttgag ctttttggaa atttcccgcg ccagatttgc
      901 cggcaagctt ccccacgaag tcgtctcgcc cgcggcgata accgcgccgc tgcgccggtc
      961 gtaaacgcga aaaacatatc tatgcgttat ccacgacagc gaaacgtaac cgatttcgac
     1021 gtataaatcg gcggattccc tgtagcgcac gagcgtcaaa ttcagctttt tccaatccgc
     1081 gcgcttcatc aattcctttt ccaaagcctg ccgcgacggt tgcagagaag atttaaccag
     1141 cgcaatcgtt ttcgccgagc gaagcgcttc ggcgggcgtt acggctttat tttcaatgaa
     1201 agctttattt tccgcgggcg tcgcgggctg actttcggaa aaagacggcg tttgcccgcc
     1261 cgcgacgttg acgggcgtgg cgcgattttt ctcaatcaag ccttgaatca cttcgtcatc
     1321 gacgccggct ttttttaatt cgatgagcgc tttcgtcgaa atatcgaagt ttccgtcggc
     1381 gctggctatt ttttgcaaaa taatctgtct gcccaatccg gcgcgcgtca tttcgacgat
     1441 ttccgtgttg gtcagagtct cggtctgcgc gaaaaccgtc gaggcgaaaa tcattaaaag
     1501 caatattgtc gagatacttt tcataaaatt ttatttttct tcgccgacgc cccggatgct
     1561 cacgcccgat ttggtttcaa aagccggaat cagcattttt cgcgagcttt tgctcaattt
     1621 taaaaccgcg taatcgtcgc ccgtgcggat ggtcagccag tgacgcctgt gtttggtaaa
     1681 ggcgacggga agcgcgacga accagagcgg cggaaacaga acgccagccg cgcccaagcc
     1741 gagcccggtc ttccagcgcg gattttttga ataagaatat tcggcgcttc tgatgtcgga
     1801 ataaagaaag gttttcgtga ttgcgccgtt tttcgaagcg atggtcagcg actcgcggtc
     1861 gagccggagc cgcacggatt tttcgccggc gttattgccg tcctgcacga gaagcttgat
     1921 ttcgttaaaa gagacgtcgg cggcacggct ctgcgctttg acgctcgtca aaaaattaga
     1981 taaaaacacg acgattagaa acaaagttat ttttttcata aatttccgat tctttaaatg
     2041 ttttctcgat tgtcagttta ttcggaaaaa cgtcgcgggt tcgggcggtt gtgacacttt
     2101 ctgaagcgtc acattttatt tgagcgcgtt ttcaaaagcg ccgcggattt cgagtcggtg
     2161 aaaagttaat ttttccagca aatcatttgt cgaggcgaga ttcattcttt cgacgatcgt
     2221 ccgcatcgcc gacggattag ccatcggcag gcgattcgtg cgcccgaccg tcaaacgcaa
     2281 actgtggtcg agacagctta aaaaatcata accgtcgtcg aaggcttgaa aatcggcttc
     2341 gtcgagcgag ccgttttcgc ggagctttcg gagcattgcg cgcgtcgagc gattttcggc
     2401 atcgtccgga acattgtcgc gcaattgcag aaagcgcatc gcaaaataaa cgtcgagcat
     2461 tccgcccgc
//
LOCUS       DASWNE010000114        14439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_251268, whole genome shotgun sequence.
ACCESSION   DASWNE010000114 DASWNE010000000
VERSION     DASWNE010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 14439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14439
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251268"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VGB68_02360"
     CDS             <1..323
                     /locus_tag="VGB68_02360"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEY0048100.1"
                     /translation="VIACGEPKFLDDEFDTLLNPVVLIEVLSPTTADYERGKKFWHYQ
                     SIESLREYLLIAQDEIRVEHLIKQADGRWQVAEYTSIEKEIELPSIEIVLKLSEIYER
                     ILKS"
     gene            326..982
                     /locus_tag="VGB68_02365"
     CDS             326..982
                     /locus_tag="VGB68_02365"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY0048101.1"
                     /translation="MEVTRLYLIRHGQSAGNAEGRFGGHSATPLSGLGKQQAELTAKT
                     LAKERISAIYSSDLLRAVQTAEPLARALNLEINAAETFRERHVGVLEGLTFDEAKKEF
                     PEDYYALVNRELAHVITRGESYRQLLDRTTGVLHEILEKHKGGRVAIFAHTGTICFLT
                     LYIIGAIHPETITTPWLITSNCGINRFEFRGRNNVRIVALNDTRHLMQITGNDAFAAR
                     "
     gene            complement(1097..2005)
                     /locus_tag="VGB68_02370"
     CDS             complement(1097..2005)
                     /locus_tag="VGB68_02370"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0048102.1"
                     /translation="MNYDVIIIGAGAGGISAACWCAELGLKALLLESRAEPGGQLLWT
                     HNPIKNHLGVEAENGREMRDIFVRQSANYNFSLRTGAEVSDADLENKIVFLQNGEAFS
                     ARALIIATGIRRRKLDVAGEQELKNKGILESGKRDAEKAKEKNVCVVGGGDAALENAL
                     ILAETARKVTLVHRRKDFRARAEFLEKARQNPSVEIFTEAFVTKIIGREKVEAVEINS
                     RNEVFILPVEAVLIRAGVEPNTALFRGKLKLDENGYIEIDSRCETSADKVFAVGDAAN
                     PLAPTISSAVGMGAIAAKVILDKLSV"
     gene            2374..4923
                     /locus_tag="VGB68_02375"
     CDS             2374..4923
                     /locus_tag="VGB68_02375"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0048103.1"
                     /translation="MKLELESTATTMQSPFAANDLSINGFDAEKINEMLKAGINAAKE
                     GNRAEARMLLLRVAESQTDNETAWLWLASISEYPEELLVFLNNVLNINPNNERALEWS
                     KATQSLLAKTFVQRGITASRENQKDFASQCFRQALAHDDRNEMAWLWLASVADSPEEK
                     TAHLEKVLSLNPENETAQASLKAVRQQASQALLKKANVAAISGEHEAARLMLDELMNL
                     SPEMEEAWILKAYLSEAHEEKIACFEKVLALNPENETAQAGIDSLNAIRQRELERAAQ
                     RAELERLQTAAAQQAQPSVSEEVPVLFFDFQSAAQKHPANEEYPDFNYMEEAEDDIHK
                     TSTANQSESGFLQDGPTQELEEEFVQNLEVQSAFMQQPESQTDGDENHQPVQEFFAEE
                     DAENNFSEPQLSEPSAETGVEESAAETATEDAQPETIFESRSDENYAYESKGFEIMTA
                     EDSYLQETASLYEEDSQSKDVEISQPASFAEDFFAPVEEATVTAQYFAANNGYQSESD
                     ALENNFSEFDPEAGQDSELLPQTGFFVRENETAAGSDFETEDEIEEICLDENETLDNY
                     FRVEDESDETRFDESDRAEPYVHEEEENPAAAQKPAAQSAAFDCPFCASVNEPQSFAC
                     PSCQAVLTLSDLEMLLSNSQADRAVLTEAIERMESEMAERQFTAEELTALGIAHLNAK
                     NLRQGFTYLQQASQMNQNNVVLGGQVNALAIRLDEIEQHESKTQEAKSCTILVVDDSP
                     TVRKLISGKLEKSGHTVVLAVDGMDALAKISEVVPDLVLLDITMPRMDGYQVCKLIRN
                     NNSTKDIPVVMISGKDGFFDKVRGRMAGSTGYITKPFGPDTLMRTIDAYLN"
     gene            5005..5565
                     /locus_tag="VGB68_02380"
     CDS             5005..5565
                     /locus_tag="VGB68_02380"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY0048104.1"
                     /translation="MQDFTPENLADIDFSSLLDLPEANSIFLAEKPVQAYGEKYVVFA
                     LDEEFYAVHSNLVAEVLSPLPVTPLPGVPEWLSGVANLRGEIISIVDLRKFWKKKSAA
                     HASAQKNKLIVLRSAKDETRVAFVIDRLSEIVTLAPQDIKFSAADFENSFPTLFGKAA
                     HKSNTLLLFDAENLLSSLSLSELQSY"
     gene            5579..7156
                     /locus_tag="VGB68_02385"
     CDS             5579..7156
                     /locus_tag="VGB68_02385"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY0048105.1"
                     /translation="MAKTSANSLLNSLKGKIWLASIGLAFFICIFGLISYLIISFLTN
                     DTLYAVFIPFVFLAFSVVIFGWWLAGEVVGPVEKVSLLAKSLERGVSAGLPKTSGSTE
                     TDELLETLQRISQQVQKLVTSMDEAANGKLEAALASNPTSDRVSQTFQKLLAKVSESI
                     HAKQDLEKLELAVSRLSGEVAGVKSGNLDAEINSDFKETKELSGAIKFLIENLSEIAA
                     LVKNNSSGAHCAVVELRKVIQELIQQDETRVQEMNQASVSLKKVPQIVQKISEELSGS
                     AQTANHSIEKARHGTQTAQQNLDAVSGLRKQIQEAATRVQRLNERSQEINKIAKTIED
                     LAQRTSLVALNASIQATELGEKGHGFAVVAEEVERLAERAGNTNKHISTLNQAIQTEI
                     GEVARSLESTVGETANLSKYAIETGNALGEMERYIATVLNLQNKVMSYTLEQTDETEK
                     AFEVFIGGISETENAAASLKESAGKLTKLADAMEELQQAVAPLKLPGSEVEETPIAPN
                     SFTGSKFPAAYEEEVSA"
     gene            7178..8710
                     /locus_tag="VGB68_02390"
     CDS             7178..8710
                     /locus_tag="VGB68_02390"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY0048106.1"
                     /translation="MTQKSIVQNLLIRISSILSVVFLLLGLVFMIADYFGVESKTFVS
                     LLVMSLAVGLSIFSIAATNFSMFKLNGEIKESAFIASQLAQGEISFDLDEDEDASEMK
                     TALKQIADNLAEKTVIADRMATGDLSANIVPHSEADVFGNAFQNMIDKLRVLVQTEDE
                     RNRLHKSITKLLNEVSEVAAGDLTVQAEVSPEMTGAIAKAFNSMTKELRSLIKQVKDV
                     TFQVSNSANAINDTTEQLARGSEAQGSQISRTTSAISSMALQIQEVSDNALLSAQVAG
                     DSLDNARYGTKAVQDNINAMNSIRNQVQETAKRIKKLGERSQEISQIVNLLDDLSDRT
                     SLLALNASLQAAAAGESGRGFAVVAEEVERLAERSNSLTKQIANLAQTIQTETKDVVA
                     SMEETIHEVVVGSTLADKAGHALVEIEQVSNRLAGLIQSISESAKQQANSSEDISKAM
                     ANISKVTELVQIGSKRAADSVKMLVVLSDELRGSVAPFKLPDEKILRRKSATETNGIY
                     VN"
     gene            8821..11685
                     /locus_tag="VGB68_02395"
     CDS             8821..11685
                     /locus_tag="VGB68_02395"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0048107.1"
                     /translation="MDAELLQGFIEEAQSYLPTIRQGIELYAADSARTEDLKTAYRQV
                     HTIKGAALMVGLAEIGAFAKTFQDELDEIVSSKAFLSKAQAADFFNKTALLEEMFANI
                     ELNAVPEEPENFELGKSENFFFEENLFEVPPPQFEPAFSEEFEIDKEMLEIFALEAEE
                     HLRTITANLNLLEQNPNNREALLEIRRSSHTLKGSAGIVGFQKLSALAHVQEDLLDYL
                     AENETGNAVDNKAVVKILMASTDCLEALSAGKDSPELEQKIAEIDAGFRQVLAQLKAS
                     EPAPLAQAKMPAPAAPNEFKTPETPNKIQMPVVQTPAAPPVGEPKIEAVESPEIHQTQ
                     SAAQNRAVIRVSLERFDNLVELVSEMVVSRSAFEQRLANLKRQVEELHHSTRRLQRLS
                     GKIETDFAAGNAHLPPPSSYSFAKEPSYPFDSPGEEFDLLEFDRYTEFNQTTRELVET
                     TSDTSAINSELDNLVANLSLLFETQRSLFDEMQDKLIRLRMVPLSTLESRLQRTVRVA
                     ADEEGKSVDLTMECDNLEIDSEILHVIVEPLLHLLRNAVAHGVESAEARELLGKPEKG
                     LITLRAYSEGTHIILNVTDDGRGIVAAALKKKAAGLGFISPAEAEAMSEEDAFSLIFL
                     PGLSTSTSVNQVSGRGVGMNVVKSSIMRQQGSISVKSVPQKGTSFTLRVPTTLAITRV
                     LLVKASGQTFAFPLKIVRRVTEISQKDFELAKRESSLNINGKIYGLAHLNELLSLPVQ
                     PLPENARVLLLLIETSENPYALAIEQILKPEEIVIKPLGNPLKSVPEFLGATILGDGS
                     VVPVLDLVPLLQKSRARIRKQSPPLENRKPKTEFSVLIVDDSPSVRQINSKIVKNAGW
                     QVLVAKDGLEALETLQAAAELPDVILTDVEMPRMDGYELLATLQRQERLRRIPVVMIT
                     SRAGEKHRRKAFDLGVSEYLTKPYEDNILLDKIKTLANFK"
     gene            complement(11687..12715)
                     /locus_tag="VGB68_02400"
     CDS             complement(11687..12715)
                     /locus_tag="VGB68_02400"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0048108.1"
                     /translation="MRKTFILLIVCLLISAAKAQTVPNDAAKADLQKTNAEIVRLFQQ
                     GKYNEALPLARKAVALTEQLFGKSDLETALALRNLGFVQNAGNDARAAEDAFEAALDI
                     YKKIPDLDKQNGATFAEMLENLAFIKFQKRMDSAESYYEMALAWREKTAGADSIKTAK
                     ALSALASISYWRKDYKKAARLFQRVLEIPGKNTENHSDETTLVYYRTECAFRKAGMED
                     EFAPLKQKYSERITLKTKDDTTKAALAPDAQIVKKGVVNGIALSLPRPAYPSEAKRVG
                     AEGRVEVQVIIDEQGTVIYACAVKYDHLSLTESSEIAAYQSKFAPTLLSGKPIRVFGT
                     VVYSFQRR"
     gene            complement(12725..13441)
                     /locus_tag="VGB68_02405"
     CDS             complement(12725..13441)
                     /locus_tag="VGB68_02405"
                     /inference="COORDINATES: protein motif:HMM:NF005594.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0048109.1"
                     /translation="MNLDGKIAVVTGGTKGIGRAVAESLLKAGASVFVCARDKSVLKH
                     ALEQLSALGKIEGEVCDVRSEAQVKMMFDEAVRVFGGVDILVNNAGVGVFGKTIDETE
                     PDEFRWVVETNLFGVFYACHHAIPLMRERGGGYIINISSLAGQNAHPKMAAYNASKFG
                     LNGFTEAMMQEVRHDNIRVSLICPGSVNTEFGGGEVSPENAWQLQPADIAQAVLDLLN
                     HNARSLPSKIELRPSQPPRK"
     gene            complement(13539..>14439)
                     /locus_tag="VGB68_02410"
     CDS             complement(13539..>14439)
                     /locus_tag="VGB68_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048110.1"
                     /translation="IFRIVLFLSFLSVVAPVAAQLPIDLQIRMEIEERKRRDRDFERL
                     RNIGGVRGRKVSDALRPTVKPTGKDLEAVAVSAEDVSRFAAFLKQPRTGIFRFHDISK
                     CEESQKVYNVEEPCPAHIPEKGSAYSFLEEDYEFKLLSDISLEKSIFRVRKLGTLSFL
                     TDLGSVSIENLTLNADGIREMAEFVPSVDKKVVVAQYKIASKGFQVGKHIYKTSHPLK
                     ENSTYALRSIAYRMQSPNMSIKTKRLDIIVIFRVVRTHGDGSVSILWKELQRKDAPKL
                     IGNKLVADGKSDIKNVPQKFAGN"
BASE COUNT         3545 a         3817 c         3714 g         3363 t
ORIGIN      
        1 tcgtcatcgc ttgcggcgaa ccgaaatttc tggacgacga attcgatacg cttttaaatc
       61 cggttgttct gatcgaagtt ctctcgccga cgaccgccga ttacgagcgc ggcaaaaaat
      121 tctggcatta tcaaagcatc gaatccctgc gcgaatatct tttgatcgcg caggacgaaa
      181 tacgcgtcga gcatttgatt aaacaggcgg acgggcgctg gcaggtggcc gaatatactt
      241 cgattgaaaa ggaaatcgag ctgccgtcaa ttgaaatcgt tttaaaatta agcgaaattt
      301 acgaacgaat tttgaaaagt tagaaatgga agtcacgcgg ctttatctca taagacacgg
      361 gcaatccgcc ggaaatgccg aagggcgttt cggcggtcat tccgccacgc cgctttccgg
      421 gctgggcaaa cagcaagccg agctgaccgc caaaactctc gccaaagaac gcatttcagc
      481 aatttattcg agcgatttac tgcgcgccgt ccaaaccgcc gagccgctgg cgcgagcttt
      541 aaatctggag attaacgccg ccgaaacctt tcgcgagcgg cacgtcggcg tgctcgaagg
      601 cttgactttc gacgaggcga aaaaagagtt tcccgaagat tattacgcgc tggtcaaccg
      661 cgagctcgca cacgtcatca cgcgcggcga aagctatcgt cagctgctcg accggacgac
      721 cggcgttctg cacgaaattc tcgaaaaaca taaaggcggg cgcgttgcga ttttcgccca
      781 caccggcacg atttgttttt taactcttta cataatcggc gcgattcacc cggaaacgat
      841 tacgacgccg tggctgatta cttccaactg cgggatcaat cgtttcgagt ttcgcgggcg
      901 gaataacgtt cgcatcgtcg cgctcaacga cacgcgccat ctgatgcaaa tcaccggcaa
      961 tgacgctttt gccgcgcgct aaagctttta tcaattacga attacaaatt acgaattacg
     1021 aattggaaga atctttcctt ttacgaattg gaagaatatt tccttcattc gtcattcgta
     1081 attcgtaatt cgtaattcaa acagacaatt tatcgagaat cactttcgcg gcaatcgcgc
     1141 ccattccgac ggcgctgctg atggtcggcg ccagcggatt tgccgcgtcg ccgacggcaa
     1201 aaactttgtc cgcgctcgtt tcgcagcggc tgtcgatttc gatgtagccg ttttcgtcga
     1261 gcttcaattt tccgcgaaaa agcgcggtgt tcggctcgac tccggcgcga atcaaaaccg
     1321 cctcgaccgg caaaataaaa acttcatttc tgctgtttat ctcgacggct tcgacttttt
     1381 cccgaccgat aatttttgtg acaaacgctt cggtgaaaat ttctacggac ggattttgac
     1441 gggctttttc gagaaattcc gcgcgggcgc gaaaatcttt tcgacgatga acgagagtta
     1501 cttttcgcgc cgtttccgcc aaaatcaaag cattttccag cgccgcgtcg ccgccgccga
     1561 cgacgcagac atttttttct tttgcttttt ccgcatcgcg cttgcccgat tccagaattc
     1621 ctttgttttt cagttcctgc tcgcccgcaa catcgagttt tctgcgcctg atgccggtgg
     1681 cgataattaa agcccgcgcc gaaaaagctt cgccgttttg caaaaagacg attttgtttt
     1741 ctaaatccgc gtccgaaact tccgcgccgg tgcgcagcga aaaattataa ttcgccgact
     1801 gcctgacaaa aatatcgcgc atctcgcgcc cgttttcagc ttcgacgccg agatggtttt
     1861 ttatcgggtt atgcgtccag agaagctgac cgccgggttc cgcccgcgat tccaaaagca
     1921 aagctttcaa accgagctcg gcgcaccagc acgccgccga gatgccgccc gcgcccgcgc
     1981 cgatgataat gacgtcataa ttcataaaaa caatccgaat ctaaaatcta aaattccttt
     2041 ggtctgattt ttgctctatt ttaaacgcgc tcaccagatt catatctttt atctcaaatt
     2101 ctcgcgaatc cagccagaaa aataccattt tttgtcaatt tctccttccg ctttttgtca
     2161 ctttgggaat tctgcatctt tcaatttagc ctcagactca tctgtaaaac cagtcaaagc
     2221 ctgatttttc ggaaaaaact ccatcttcgg cacgcgattt ggagtttttt ccgttatggc
     2281 atttgtcttg ctgtcgtaag gtcgatttgg tcgttttccc gacttctcaa atcaaagtct
     2341 caaatcattc acaaagccgg atttctgaaa actatgaaat tagaacttga atcaaccgct
     2401 acgactatgc aaagtccatt tgccgccaat gatttatcaa tcaacggttt tgacgccgag
     2461 aagataaacg aaatgttgaa agccggcatc aatgccgcca aagaaggcaa tcgcgccgaa
     2521 gcgagaatgc tgctcttgcg cgtcgccgaa agtcagaccg ataacgaaac cgcctggctg
     2581 tggctcgcct caatcagcga atacccggaa gaactgctcg tttttctaaa caacgttctg
     2641 aacatcaacc cgaacaacga gcgcgccctt gaatggtcga aagcgaccca atcgctgctc
     2701 gccaaaactt ttgttcaacg cggcattacc gcctcgcgcg aaaaccagaa ggatttcgcc
     2761 agccagtgtt ttcggcaggc tctcgcgcac gacgaccgaa acgagatggc gtggctgtgg
     2821 ctggcttcgg tcgccgattc gccggaagaa aaaacggcgc atctcgaaaa agttttatcg
     2881 ctgaatcccg aaaacgaaac ggcgcaagcc tcgctcaaag ccgtcaggca gcaggcttcg
     2941 caggcgcttc tgaaaaaggc gaacgtggcg gcaatctccg gcgagcacga agcggcgcga
     3001 ttgatgctgg acgaattgat gaatttatcg cccgaaatgg aagaagcgtg gattttaaaa
     3061 gcttatcttt ccgaagcgca cgaagaaaaa atcgcttgtt tcgaaaaggt tctggcgctc
     3121 aacccggaaa atgaaaccgc gcaggcgggc atcgactcgc tgaacgccat ccggcagaga
     3181 gaactcgagc gggcagcgca gagagcggaa ctcgaaagac tccagacggc ggcggctcag
     3241 caggcgcagc cgtctgtttc cgaagaagtt ccggtgttgt tttttgactt ccagtcagcg
     3301 gcgcagaaac atccggcaaa cgaagaatat cctgacttca attatatgga agaggcggaa
     3361 gacgacattc ataaaacttc aactgccaat caatcggaat ccggcttttt gcaggacggt
     3421 ccgacgcagg aactggaaga ggaatttgtg cagaatctgg aggttcagtc cgcttttatg
     3481 cagcagcccg aatctcagac cgacggcgac gaaaaccatc agccggttca ggaatttttc
     3541 gcagaagaag acgcggaaaa taatttctcc gaaccgcagc tttccgagcc gtcagccgaa
     3601 accggagtcg aagaatcggc ggcggaaacg gcgaccgaag acgctcagcc ggaaacgatt
     3661 ttcgaatctc ggagcgatga aaattacgct tacgaatcga agggttttga aataatgacc
     3721 gcagaagatt cttaccttca ggaaaccgct tctttatacg aagaagattc gcagtcgaaa
     3781 gacgtagaaa tttctcaacc ggcaagcttc gccgaagatt tttttgcgcc cgtcgaagag
     3841 gcgaccgtaa cggcgcaata tttcgcggcg aacaacggat atcagtctga aagcgacgcc
     3901 cttgaaaata atttttccga attcgacccg gaagcgggac aagattccga actccttccg
     3961 cagaccggat ttttcgtcag ggaaaacgaa actgcggctg gcagcgattt tgaaacggaa
     4021 gacgagattg aggaaatctg tcttgacgaa aacgaaacgc tcgacaatta ttttcgggtc
     4081 gaagacgaaa gcgacgaaac ccgttttgac gaaagcgacc gcgccgagcc gtatgtccat
     4141 gaagaagaag aaaatccggc tgcggcgcaa aaacccgcgg cgcagtccgc cgccttcgac
     4201 tgcccgttct gcgcgtccgt taacgagccg caatcgtttg cgtgcccgtc gtgccaggcg
     4261 gttttgacgc tttcggattt ggagatgctg ctctcgaaca gccaagccga ccgcgccgta
     4321 ttgacggaag cgattgaaag aatggaaagc gaaatggcgg agcgccagtt tactgccgaa
     4381 gaattgaccg cgctcggtat cgctcattta aacgcgaaaa atctgcgtca gggcttcaca
     4441 tatttgcagc aagcctcgca gatgaatcaa aacaacgtcg ttctgggcgg tcaggtcaac
     4501 gcgctggcga ttcgactgga cgaaatcgag cagcacgaaa gcaaaacgca ggaagcgaaa
     4561 agctgcacga ttctggtcgt ggacgacagc ccgaccgtta gaaaactgat ttccggcaag
     4621 ctcgaaaaaa gcggtcacac cgtcgttctg gcggttgacg gaatggacgc gctggcgaaa
     4681 atcagcgagg tcgttcccga tttggttttg ctcgacatta cgatgccgcg gatggacggt
     4741 tatcaggttt gcaagcttat ccgcaacaac aactcgacga aagacattcc ggtcgtaatg
     4801 atttccggca aagacggctt tttcgacaaa gttcgcgggc ggatggctgg ctcgaccggc
     4861 tacattacga agcccttcgg acccgacacg ctgatgcgga cgattgacgc ttatttgaat
     4921 taaaataaaa aggcaaaagt aaaaaggcaa aattttgatt tcttactttt gccttttgcc
     4981 ttttgccttt tatcttctta ttttatgcag gatttcacgc ccgaaaactt ggctgacatt
     5041 gatttttcct cactgctgga tttgccggaa gccaactcga tttttctcgc cgaaaaaccc
     5101 gtgcaggctt acggcgaaaa atacgtcgtt ttcgcgctcg acgaagaatt ttacgccgtt
     5161 cactcaaacc tcgtcgccga agttttaagt cctctgccgg tgacgccgct gcccggcgtt
     5221 cccgaatggc tttcgggcgt cgccaatctg cgcggcgaga taatctcgat tgtcgatttg
     5281 cgaaaattct ggaaaaagaa atcagccgct cacgcttccg cgcagaaaaa caagctcatc
     5341 gtgctccgct ccgctaaaga cgagacaagg gtcgcgtttg tcatcgaccg cctgagcgaa
     5401 atcgtcaccc tcgcgccgca ggacattaaa ttttccgccg ccgattttga aaattctttt
     5461 ccgaccctgt tcgggaaagc ggctcataaa tcgaacactc tgcttttatt cgacgcggaa
     5521 aaccttctct cgtcgctcag cctcagcgag cttcaatcct attaaccgga aagaaaatat
     5581 ggcaaaaact tctgcaaatt ccctgctcaa ttccttgaaa gggaaaatct ggctcgccag
     5641 tatcgggctg gcttttttta tttgcatctt cggcttgatt tcctatctga taatttcgtt
     5701 tttgactaac gacacgcttt acgccgtttt tattccgttt gttttcctcg ctttttcggt
     5761 ggtcatcttc ggctggtggc tggcgggcga ggtggtcggt ccggtcgaaa aagtttcgct
     5821 gctggcaaaa agtctggaac gcggagtctc cgcgggcttg ccgaaaactt ccggctcgac
     5881 cgaaaccgac gagcttctgg aaacattgca gcgcatcagc cagcaggtgc agaaactcgt
     5941 cacctcgatg gacgaagcgg caaacggcaa gctcgaagcc gcccttgctt cgaatccgac
     6001 gtccgaccgc gtcagccaga ctttccaaaa acttctcgcc aaagtctccg agtcgattca
     6061 cgccaaacag gatttggaaa aactggagct ggctgtcagc cggctttccg gcgaggtcgc
     6121 gggcgtcaaa tccggcaatc tcgacgccga aataaactcg gattttaaag aaaccaaaga
     6181 gctttcaggg gcgattaaat ttcttatcga gaatttaagc gaaatcgccg cgctcgttaa
     6241 aaacaattct tccggcgcgc attgcgcggt cgtcgaattg cgaaaagtga ttcaggaatt
     6301 gattcagcag gacgaaacca gagtgcagga aatgaatcag gcttcggttt cgctgaaaaa
     6361 agttccgcag attgtccaga aaatttccga agagctttcc ggctcggcgc aaaccgccaa
     6421 tcattcaatc gagaaggctc gccacggaac gcagacggcg cagcaaaatc tggatgccgt
     6481 cagcggcttg cgcaaacaaa ttcaggaagc cgcgacccgc gtccagcgct tgaacgagcg
     6541 ctcgcaggaa atcaacaaaa tcgctaaaac gattgaagat ttggcgcagc gaaccagtct
     6601 ggtcgcgctt aacgcttcga ttcaggcgac ggagctcggc gaaaaaggtc acggttttgc
     6661 cgtcgtcgcc gaagaagtcg agcggctggc ggaacgcgcc ggaaatacca acaagcatat
     6721 ctcgacgctc aaccaggcga ttcagacgga aatcggcgag gtcgcccgct cgctggagtc
     6781 gactgtcggc gaaacggcga atctttccaa atacgcgatt gaaaccggca acgcgctcgg
     6841 cgagatggag cgatacatcg ccacggtttt gaatttgcag aacaaagtga tgtcttacac
     6901 gctcgagcag acggacgaaa cggaaaaggc tttcgaagtg tttatcggcg gcatttcgga
     6961 gacggaaaac gcggcggcaa gcctgaaaga atccgccgga aagctgacaa agctggctga
     7021 cgcgatggag gaattacagc aggcggtcgc gccgctcaag ctgccgggca gcgaggtcga
     7081 agaaacgcca atcgcgccga attcgtttac cggttcaaaa tttcccgccg cttacgaaga
     7141 agaagtttcc gcataaaagt ttaccggagc aaatcaaatg acgcaaaaat ccatcgtgca
     7201 aaacctttta atcagaattt cgtcgattct ttccgtcgtt ttcctgctgc tcggcttggt
     7261 ttttatgatt gcggattatt tcggcgtcga atcgaagact ttcgtcagcc tgctggttat
     7321 gtctctggcg gtcgggctgt cgattttctc gattgcggcg acgaattttt cgatgtttaa
     7381 gctgaacggc gagattaaag aatcggcttt tatcgccagc cagctcgcgc agggcgaaat
     7441 ttccttcgat ttggacgagg acgaagacgc gagcgagatg aaaacggcgc tcaaacaaat
     7501 cgccgacaat ctggcggaaa aaaccgtgat tgccgaccgt atggcgaccg gcgatttatc
     7561 ggcgaatatc gttccgcatt ccgaggctga cgttttcggc aacgctttcc agaatatgat
     7621 tgataaactg cgcgtgctgg ttcagaccga agacgagcgg aaccgtctgc acaaatcaat
     7681 caccaaatta ttaaacgaag tttccgaagt cgccgccggc gatttgaccg tgcaggcgga
     7741 agtctcgccc gagatgaccg gcgcaattgc caaagccttt aattcgatga ccaaggaatt
     7801 gcgctcgctc atcaagcagg tcaaggacgt gacttttcag gtcagcaatt cggcaaacgc
     7861 catcaacgac acgaccgagc aactggcgcg cggctctgag gcgcagggct cgcagatttc
     7921 gcgcacgact tcggcgattt ccagtatggc tttgcagatt caggaagttt ccgacaacgc
     7981 tctgctttcg gcgcaggtcg cgggcgattc cttagacaac gcgcgctacg gcacgaaagc
     8041 cgtgcaggac aacatcaacg cgatgaactc gattcgcaat caggttcagg aaacggcgaa
     8101 acgcatcaaa aagctcggcg agcgctcgca ggaaatcagc cagattgtca atctgctcga
     8161 cgacctttcc gaccgcacga gccttttggc tttaaacgcg agcctgcagg cggcggcggc
     8221 tggcgaatca ggtcgcggtt ttgccgtcgt cgccgaagaa gtcgagcggc tggcggaacg
     8281 ctcgaacagt ttaaccaaac aaatcgccaa tctcgcgcag acgattcaga cggagacgaa
     8341 agacgtcgtc gcctcgatgg aagagacgat tcacgaggtc gtcgtcggct cgacgctcgc
     8401 cgacaaagcc ggacacgcgc tggtcgaaat cgaacaggtc tccaaccggc tcgccggatt
     8461 gattcagtcg atttccgaat ccgccaaaca gcaggcgaac agctcggaag acatctcgaa
     8521 agcgatggct aatatctcga aggtgacgga actcgtacaa atcggctcga aacgcgccgc
     8581 cgattcggta aaaatgctcg tcgttctatc ggacgagctg cgcggctcgg tcgcgccctt
     8641 caaactgccg gacgagaaaa ttctgcggcg aaaatccgca acggagacaa acgggattta
     8701 cgttaattga tataactgga aagagaatga aaccacggat ttacacggat aaactcagat
     8761 gttttcatct cttttttatc ttgtgtccgt ctgtgtaaat ctgtggtttc aaatttattt
     8821 atggacgctg aacttttaca gggatttatc gaagaagcgc agagctatct gccgacgatt
     8881 cgtcagggca tcgagcttta tgcggcggac agcgcgcgga cggaagattt aaaaaccgct
     8941 tatcgacagg tgcacacgat aaaaggcgcg gctttgatgg tcgggctggc ggaaatcggc
     9001 gcgtttgcca agacgtttca ggacgagctg gacgagattg tttcctcgaa agcttttttg
     9061 tcaaaagcgc aggcagctga tttttttaat aaaaccgcgc tgctcgaaga aatgttcgcc
     9121 aacatcgaat taaacgccgt gccggaagag ccggaaaatt tcgagttggg aaagtctgaa
     9181 aattttttct ttgaggaaaa tttatttgaa gttccgccgc cgcagtttga gcctgctttt
     9241 tccgaggagt ttgaaatcga caaggaaatg ctcgaaattt tcgcgctcga agccgaagag
     9301 catctgcgca ccatcaccgc caatctcaat cttttggaac aaaatcccaa caaccgcgag
     9361 gcgctgctgg aaattcgccg cagctcgcac actctgaaag gttcagccgg aatcgtcggt
     9421 tttcagaagc tctccgcgct ggctcacgtg caggaagatt tgctcgatta tctggctgaa
     9481 aatgaaaccg gaaacgccgt cgataacaaa gccgtggtca agattctgat ggcttcgacc
     9541 gattgtctgg aagctttaag cgcgggcaag gattcgcccg agctcgaaca aaaaatcgcc
     9601 gaaatcgacg ccggcttccg gcaggtttta gcgcagctga aagcctctga gccagcgccg
     9661 cttgcgcaag cgaaaatgcc cgcgccggct gctccaaacg aatttaaaac gccggaaact
     9721 ccaaataaaa ttcaaatgcc ggttgttcaa actccggctg ctccgccggt cggcgagccg
     9781 aaaatcgaag cggtcgaatc gccggaaatt caccagacgc aaagcgccgc tcaaaatcgc
     9841 gcggtcattc gcgtctcgct cgaaagattt gataatttgg tcgagctggt cagcgagatg
     9901 gtggtcagcc gctcggcttt cgagcagcgt ctggcgaatc ttaaacgaca ggtcgaagag
     9961 cttcatcaca gcacgcgccg gctgcagcgg ctgagcggaa aaatcgaaac cgatttcgcc
    10021 gccggaaacg cgcatctgcc gccgccgtcg tcctacagct ttgcgaaaga gccgtcttac
    10081 ccgtttgact cgcccggcga agaattcgat ttgctcgaat tcgaccgtta cacggaattt
    10141 aatcaaacga cgcgcgagct ggtcgaaacg acttccgaca catccgccat taacagcgag
    10201 ctggataatc tggtcgctaa tctgagcctg cttttcgaga ctcagcgcag cttgtttgac
    10261 gagatgcagg acaagcttat aagactcagg atggttccgc tgtcgacgct ggaatcgcgt
    10321 ctgcagcgca cggttcgcgt ggcggctgac gaggaaggaa aatcagtcga tttgacgatg
    10381 gaatgcgaca atctggagat tgattcggaa attctgcacg tgattgtcga gccgcttctg
    10441 catcttttgc gcaacgccgt cgcgcacggc gtcgaatccg ccgaagccag agagctgctg
    10501 ggaaaaccgg aaaaaggtct tatcacgctg cgcgcctaca gcgaaggcac gcacattatt
    10561 ttaaacgtta cggacgacgg gcgcggcatt gtcgccgccg cgctcaagaa aaaagccgcc
    10621 ggactcggct ttatctcgcc cgccgaagcc gaggcgatgt ccgaagaaga cgctttttcg
    10681 ctgatttttc tgcccgggct ttccacctcg acttccgtca atcaggtttc ggggcgcggc
    10741 gtcgggatga acgtcgtgaa atcgagcatt atgcgccagc aaggctcgat ttccgtcaaa
    10801 agcgttccgc agaaaggcac cagctttacg cttcgagtcc cgacgacgct cgccatcacg
    10861 cgcgttttac tggtcaaagc ctccggtcag acattcgctt ttccgctcaa aatcgttcgc
    10921 cgcgtgacgg agatttcgca gaaagatttt gaactggcga aacgcgaaag ctcgctgaat
    10981 atcaacggca aaatttacgg gctggctcat ctgaacgagc ttttgagctt gccggtgcag
    11041 ccgctgccgg aaaacgcgcg cgttttgctt ttgctgatcg agacttcgga gaatccttac
    11101 gcgctggcaa tcgagcagat tctgaaaccc gaagaaatcg tcatcaaacc gctcggcaat
    11161 cccttaaaaa gcgttcccga atttctgggc gcaactattt taggcgacgg ctcggtcgtg
    11221 ccggtgctcg atttggttcc gcttttgcag aaaagtcgcg cgcggattcg aaaacagagc
    11281 ccgccgctcg aaaaccggaa accaaaaacc gagttttccg tcttgattgt cgacgacagc
    11341 ccgagcgtca ggcaaatcaa ctcgaagatt gtcaaaaatg ccggctggca ggtgctggtc
    11401 gccaaggacg ggctggaagc gctggaaact ctgcaagccg ccgccgagct gccggacgtg
    11461 attttaaccg atgtggaaat gccgcggatg gacggctacg agctgctcgc cacgcttcag
    11521 cgtcaggaac gcctgcgccg gattccggtc gtgatgatta cctcgcgcgc gggcgaaaaa
    11581 catcgccgga aggctttcga tctgggcgtg tcggagtatc tgaccaaacc ttacgaagat
    11641 aatattctgc tggataaaat aaaaacttta gctaatttta aataaactat cggcgctgaa
    11701 agctatagac gaccgttccg aataccctga tcggttttcc gcttaataaa gtgggcgcaa
    11761 attttgattg ataggcggca atctccgagc tttccgtcaa actaaggtgg tcgtatttga
    11821 cggcgcaggc gtaaattacc gtgccctgct cgtcgattat cacctgaact tcgactctcc
    11881 cttccgcgcc gacccgtttt gcctcactcg ggtaagccgg tctcggcaag ctgagagcaa
    11941 tcccgttaac cactcctttt tttactatct gcgcgtccgg cgcgagggcg gctttcgtcg
    12001 tatcgtcttt tgttttaagg gttatccgct cggaatattt ttgtttgagc ggcgcaaact
    12061 catcttccat cccggctttt ctgaacgcgc actcggtgcg gtaataaacg agcgtggttt
    12121 cgtcgctgtg attttccgta tttttgcccg gaatctccag cacgcgctga aaaaggcgcg
    12181 cggctttttt ataatctttt ctccaatagc tgatgctggc taaagcggac aaagccttgg
    12241 cggttttaat cgagtcggcg ccggctgttt tctcgcgcca ggctaaagcc atttcgtaat
    12301 agctttcggc tgaatccatt cgcttttgaa attttataaa agcgagattt tccagcattt
    12361 cggcaaatgt cgcgccgttt tgtttatcca aatcgggaat ttttttgtaa atgtcgagcg
    12421 ccgcttcaaa agcgtcttcc gccgcgcgcg cgtcatttcc ggcattttga acaaagccta
    12481 aatttcttaa ggcgagcgcc gtctcgaggt cgcttttacc gaataattgt tcggtcagcg
    12541 caaccgcttt ccgcgccagc ggcagggctt cgttgtattt tccctgttgg aaaagcctga
    12601 cgatttcggc gtttgtcttc tgcaaatcgg ctttggcggc atcgtttggc acagtctgag
    12661 ctttcgccgc cgaaatcaat aagcagacga ttagtaaaat aaaagttttt cgcataaatt
    12721 atctttattt tcgcggcggc tggctcggac gaagctcgat tttgctcggc aggctgcgcg
    12781 cgttgtgatt gagcaaatcc agcaccgcct gagcgatgtc ggcgggctgc aattgccagg
    12841 cgttttccgg cgaaacttct ccgccgccga actcggtgtt gaccgagccc ggacaaatca
    12901 ggctgaccct gatgttgtcg tgccgcactt cctgcatcat agcttcggta aagccgttta
    12961 agccgaattt tgaagcgttg taagccgcca ttttcggatg cgcattttgt ccggcgagcg
    13021 aggaaatatt tatgatgtag ccgccgccgc gctcgcgcat cagcggaatc gcgtgatgac
    13081 aggcgtagaa aacgccgaac agatttgttt ccacaaccca tcgaaactcg tccggctcgg
    13141 tttcgtcgat tgttttgccg aacacgccga cgcccgcgtt attgaccaga atatcgacgc
    13201 cgccgaagac gcgcacggct tcgtcaaaca tcattttcac ctgcgcttcc gagcgcacgt
    13261 cgcaaacttc gccttcaatt ttgccgagag cggaaagctg ttcgagcgcg tgtttgagaa
    13321 cggatttatc gcgggcgcaa acgaaaacgc tcgcgcccgc ttttaataaa ctttcggcga
    13381 cggcgcgccc gatgcctttc gtgccgccgg tgacgacggc aattttacca tccaaattca
    13441 tctgatttta aaattacctg taaaggttat tgcagccgaa tccgcgcggc gcagcgcgcg
    13501 tcgccaagcc ggaataatct tttttgccaa tcgccgattt aatttccggc gaacttttgc
    13561 gggacgtttt tgatgtccga cttgccgtcg gcgaccaatt tattgcctat cagtttcggc
    13621 gcgtcctttc tttgcagctc tttccacaaa atgctgacgc tgccgtcgcc gtgcgtcctg
    13681 acgacgcgaa agataacgat aatgtccagc cgcttggttt tgatggacat attcgggctt
    13741 tgcattcgat aggcgatgga gcgcagcgcg taggtggaat tttccttgag cgggtgcgac
    13801 gttttgtaga tgtgttttcc gacctgaaat cctttcgagg cgattttgta ttgagcgacc
    13861 accacttttt tatcgaccga aggaacaaac tccgccattt cccgaatccc gtcggcattg
    13921 agagtcagat tttctatcga cacgctgccc aaatcggtca gaaagcttaa tgtgccgagc
    13981 ttccgaacgc gaaaaatgga cttttcgagc gagatatccg acagcagctt aaactcgtaa
    14041 tcttcttcca gaaaagaata ggcgctccct ttttcgggaa tatgtgccgg acacggttct
    14101 tcgacgttgt aaactttctg gctctcttcg catttcgaga tgtcgtgaaa ccggaaaatc
    14161 ccggtgcgcg gttgttttag aaaggcggcg aatctgctca cgtcttcggc gctgacggcg
    14221 acggcttcga ggtctttccc ggtcggcttg acggtcggtc ttaaggcatc gcttactttt
    14281 ctgcctctta cgccgccaat atttctcaaa cgctcgaaat cccggtcgcg ccgcttgcgc
    14341 tcttctatct ccatccgtat ctgtaaatcg atcggaagct gtgccgcaac aggggcgaca
    14401 acgctcagga aactcaaaaa caacacgatt cgaaagatt
//
LOCUS       DASWNE010000115         5298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_3914, whole genome shotgun sequence.
ACCESSION   DASWNE010000115 DASWNE010000000
VERSION     DASWNE010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3914"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VGB68_02415"
     CDS             complement(<1..426)
                     /locus_tag="VGB68_02415"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048111.1"
                     /translation="MFKKILILSASVGNGHTRAAQALEKTFRLKNAAAEIRHEDTLDF
                     TNPLFRQIYGKSYIDLVNNMPEVLGWMYDQMDEPWKNEKRRLFLEKMNTQPLVKMLRE
                     YQPDWIVCTHFLPAEIISDLKTKGKLNCPQAIVVTDFDLH"
     gene            542..985
                     /locus_tag="VGB68_02420"
     CDS             542..985
                     /locus_tag="VGB68_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0048112.1"
                     /translation="MSITFRQIKIKDFEFLWQLHNAALKKYITATWGWNEDWQRRDFE
                     RNFNPDEGEIVVIDGKDAGFLRISEKETEIALISVRLLPEFQNRGVGTKLIKEVLVEA
                     RAKNKPARLQVLKINPAQNLYKKLGFKVFGETATHFLMRVAPEEI"
     gene            1151..2794
                     /locus_tag="VGB68_02425"
     CDS             1151..2794
                     /locus_tag="VGB68_02425"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEY0048113.1"
                     /translation="MRRIFDLFGLAAVFGILCLSSVAGAEISFTATLSGAQEVPPTAS
                     SAKGFGIVTLNDAETQIRFSLTFSGLGSSQTASHIHGAAPLGANAPVLFNIGSTGSTG
                     GTFTALTQAVTPAQVQQLKSGLWYFNVHSNALPTGEIRGQILPAPPFIANLSGLQEVP
                     RNNSAGTGIGKVFLNATQDQIVMSLSFSGLGSEQTAAHIHGASLPGVNAPVLFNIGSQ
                     GMTSGSFPDLFFAVTPQQAANLKAGLFYFNVHTTALPGGEIRGQIKPPNKPLDFDADS
                     RSELAHFNSGHWILISSINRVGTSTRWGLPTDVRAPGDFDLDGKIDLNVWRNGTFYTL
                     RSSDNTFFAFQFGASGDDARICADYDGDGKADYAVYRPGANAAASSFYYIQGSLRGFM
                     AAQFGLGGDASMLADWDGDRISDITVYRQSTGTFYGVRSSDQVFYGQQWGDFSRDRVM
                     TGDIDGDGKSDPLVYRTTGADAGYWYALASSTQAFIAVKFGVGNDTPVPADFDGDGKT
                     DFAVYRSVQTAWYIAGSTGAFMEYFSPGGGVPVHSYLVR"
     gene            2902..3879
                     /locus_tag="VGB68_02430"
     CDS             2902..3879
                     /locus_tag="VGB68_02430"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEY0048114.1"
                     /translation="MRDVYTYLYDARGNDKEITFTGVESIQLRKSHLLWVNVLRRDED
                     LIKQVTARLGLENVPIKAIMNLNERPTLDNFDNYYRFSISSVELSDSNRLKRVPICYL
                     IGENFILTIHDGDINYFLEFRDREKGETQIGDLDAESFLATLLDLHLVTYFRALEKIE
                     DAVDKLDVDILRKDLRDEDFLKETVRLKSDVAKLRRWLLPHRDVFYALTRSDFQPVAE
                     SNSTKDFQRLTQHFENAVDAVETSRDTVLSLFDLYATRSAHKMNGFIKRLTFVTLIVG
                     TLGAVAGIWGMNFEVGYFKAAEQGFWMTIGGMGLFILISFVLGKIFQWI"
     gene            3941..>5298
                     /gene="argH"
                     /locus_tag="VGB68_02435"
     CDS             3941..>5298
                     /gene="argH"
                     /locus_tag="VGB68_02435"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEY0048115.1"
                     /translation="MTEAKNLWGGRFTEKPDETFAEFNNSFRFDRRLFAADIRASVAH
                     AEALFHAGVLTRLEAEKIKNGLWTILKRADYDRNYFNDSDAEDVHSFIESRLVQLIGD
                     TGRKLHTGRSRNDQVATAFRLWLREEIIEISRIARELQRALIEAAERQKEAVLPGYTH
                     LQRAQPVLWAHWCLAYFEMLDRDRERLDEVWRRVNVLPLGSAALAGTSYEIDREAVAR
                     ELGFEGVTANSLDAVSDRDFCVEFVGACSLLMVHLSRLAEDLILYASSEFRFVELSDA
                     VSTGSSLMPQKKNPDALELIRGKAGRIFGHQTALLTTLKGLPLAYNKDLQEDKEAVFD
                     TIDNVKMCLKVACIVLKNLRLDEERTRLAATQGYLNATELADYMVQKNVPFRTAHDLT
                     GKIVLYAISVGRELNDLTLAEMQKFTAFIEKDVFRALSLEQTLSSKSQIGGTAPERVF
                     EAL"
BASE COUNT         1254 a         1323 c         1437 g         1284 t
ORIGIN      
        1 gtgtaaatcg aaatccgtca cgacgatcgc ctgcgggcag tttaattttc ctttcgtttt
       61 caaatcggag atgatttcgg cgggcagaaa atgcgtgcag acaatccagt cgggctggta
      121 ttcgcgcagc attttcacca gcggctgcgt gttcatcttt tccagaaaca agcgccgttt
      181 ttcgtttttc cagggctcgt ccatctggtc gtacatccag ccgagaactt ccggcatatt
      241 gttgaccaaa tcgatgtagg attttccgta aatctgcctg aaaagcgggt tggtgaaatc
      301 cagtgtgtct tcgtgacgaa tctccgccgc cgcgtttttc agccgaaaag ttttttcgag
      361 cgcctgcgcc gcgcgcgtgt gcccgttgcc gaccgaagcc gaaagtatca agattttttt
      421 gaacatttaa tttatttaat ttagtggtaa gcggtaaacg gtaagcggca ggttcgttat
      481 tttgaaactg caactttatt tattgcaact taccacttac cgtttaccgt ttaccgttta
      541 tatgtcgatt acttttcgcc agataaagat aaaggatttt gagtttttat ggcaactgca
      601 taatgccgct ctgaaaaaat atattaccgc gacctggggc tggaacgaag actggcaaag
      661 gcgcgatttc gaaaggaatt ttaatccgga cgaaggcgaa atcgtcgtga ttgacggaaa
      721 agacgcggga tttttacgga ttagcgaaaa agaaacggaa atcgcgctga tttcggttcg
      781 ccttttgccg gagtttcaga atcgcggcgt cggcacaaaa ctgataaaag aagttctcgt
      841 cgaagcgcgg gcgaaaaaca agccggcgcg cttacaggtt ttgaaaataa atcccgcgca
      901 aaatttatat aaaaaactcg gctttaaagt tttcggcgaa accgcgacgc attttttgat
      961 gagagtcgcg ccggaggaaa tttaagcgcc gatgtttgcc gaaaaagggg cgcggcattt
     1021 ttcggtcggg tcgccgcttt tcccgaaaaa tcttgctttt atttcaataa agaattataa
     1081 gacataagtc gtaccattag tttaatcccc cgctttccga taagtttttc tttttgaaag
     1141 gagttttgcc atgagaagaa tatttgattt attcggtttg gcagccgtct tcggcatttt
     1201 atgcctgtcg agcgtcgccg gagcggagat aagttttacc gctacattaa gcggggcgca
     1261 ggaagttccg ccgactgctt cgtccgccaa aggcttcggc atcgtgacgc tcaacgacgc
     1321 cgaaacgcag attcgttttt cgctcacctt ttccggtctg ggcagcagcc agacggcttc
     1381 gcacatacac ggcgcagcgc cgctcggcgc aaacgctccg gtgcttttta atatcggctc
     1441 gacgggctcg accggcggaa cgtttaccgc tctgacgcaa gccgtcacac cggcgcaggt
     1501 gcagcagctg aaaagcgggc tctggtattt caacgttcat tcgaacgctt tgccgaccgg
     1561 cgagatacgc ggtcagattc tgcccgcgcc gccgtttatc gccaatctga gcggtttaca
     1621 ggaagttccg cgcaacaatt cggcgggaac cggaatcggc aaggtttttc tgaacgcgac
     1681 gcaagaccag attgtaatgt ctttgagctt tagcgggctc ggcagcgagc agacggcggc
     1741 gcatattcac ggcgcgtcgc tgccgggcgt aaacgcgccg gttctgttca atatcggctc
     1801 gcagggcatg acttccggca gctttcccga tttatttttc gcggtcacgc cgcagcaggc
     1861 ggctaattta aaagccggac tgttttactt caatgttcac acgacggctc tgccgggcgg
     1921 cgaaatccgc gggcaaatca agccgccgaa caaaccgctg gattttgatg ccgactctcg
     1981 ttccgagctc gcacatttta attccggaca ctggattctg ataagcagca ttaaccgggt
     2041 agggacgagc acccgctggg gattgccgac cgacgtgcgc gcgccgggcg attttgattt
     2101 ggacggaaaa attgatttaa acgtctggcg aaacggcacg ttctacactc tgcgcagctc
     2161 ggataatacg ttttttgcgt ttcaattcgg cgcttcgggc gacgatgcgc gcatctgcgc
     2221 cgattacgac ggcgacggca aagccgatta cgcggtttat cgtccgggtg cgaacgccgc
     2281 cgcgtcgagc ttttactata ttcagggcag cctgcgcgga tttatggcgg ctcaattcgg
     2341 gctcggcggc gatgcttcga tgctcgccga ttgggacggc gaccgtatca gcgacatcac
     2401 ggtttatcgt caaagcacgg gaacgtttta cggcgtcaga agctccgacc aggtgtttta
     2461 cgggcagcag tggggcgatt tttccagaga cagggtaatg accggcgaca ttgacggcga
     2521 cggaaaatcc gacccgctcg tttatcgcac gacgggcgcc gacgccggat actggtatgc
     2581 tctcgccagc agcacgcagg cgtttatcgc cgtaaaattc ggcgtcggaa acgatacgcc
     2641 cgtcccggcg gatttcgacg gcgacgggaa aacggatttc gccgtttatc gttccgtgca
     2701 gaccgcctgg tatatcgccg gttcgacggg cgcgtttatg gagtattttt cgcccggcgg
     2761 cggcgtgccg gtgcattctt atctggttcg ttaaatgccg gaaaagcgcg taaaaacggc
     2821 gagtcgcccg gctcgccgtt ttttttcaga aattttttta tattttcggc tataatcgct
     2881 aaaaagcgga gaatcgaatt aatgcgcgat gtttacacat acctttacga cgccagagga
     2941 aacgacaagg aaataacatt taccggcgtc gaatccattc aattaagaaa aagtcatctt
     3001 ctctgggtca atgttttgcg gcgcgacgaa gaccttatca agcaggtgac ggcgcggctc
     3061 ggactggaaa acgttcccat taaagcgatt atgaatttaa acgagcgacc gacgctcgat
     3121 aatttcgaca actattatcg cttttccatc agctcggtcg aactaagcga cagcaatcgg
     3181 ctcaaaagag ttccgatttg ttatctaatc ggagaaaatt ttatcctgac gattcacgac
     3241 ggcgatatta attattttct ggagtttcgc gaccgcgaaa aaggcgaaac gcaaatcggc
     3301 gacctcgacg ccgagagctt tctggcgacg ctgctcgatt tgcacctcgt cacgtatttt
     3361 cgcgcgctgg aaaaaatcga ggacgcggtc gataaactcg acgtcgatat tttgcgaaag
     3421 gatttaagag acgaagattt tctgaaagaa accgttcgcc tgaaaagcga cgtcgccaag
     3481 ctgcgccgct ggcttttgcc gcaccgcgac gttttctacg cgctgactcg ctctgatttt
     3541 cagcctgtcg ccgaatcgaa ttcgacaaag gattttcaga ggctgacgca gcattttgaa
     3601 aacgcggtcg acgccgtcga aacatcgcgc gatacggttt taagtctttt cgatttatac
     3661 gcgacgcgct cggcgcacaa aatgaacggt tttattaaaa gactcacgtt cgtcaccttg
     3721 atagtcggaa cgctgggcgc tgttgccggt atttggggta tgaatttcga ggtcggatat
     3781 ttcaaagcgg cggaacaggg attttggatg accatcggcg gaatgggatt atttatttta
     3841 ataagcttcg tcctgggcaa aattttccaa tggatttaaa aaatcggaat tgccggattt
     3901 ttagaatttc gcctacaata aacgtcacac ggagaaaaaa atgaccgaag cgaaaaatct
     3961 ctggggcggt cgatttaccg aaaagcccga tgaaactttt gccgagttta ataattcctt
     4021 ccggtttgac cggcgtcttt tcgccgccga catacgcgcc agcgtcgccc acgccgaagc
     4081 gctttttcac gccggagttc tgacgcggct cgaagccgaa aaaatcaaaa acgggctgtg
     4141 gacgattctc aagcgcgccg attacgaccg caattatttt aatgactcgg acgccgaaga
     4201 cgtgcattcg tttatcgaat cgcggctggt tcaattaatc ggcgacacgg gcagaaagct
     4261 gcacaccggc agaagtcgca acgaccaggt ggcgacggct tttcgtctct ggctgcgcga
     4321 ggaaatcatc gaaatttccc gcattgcgcg cgagctgcaa agggctttga ttgaggcggc
     4381 tgaaagacaa aaagaagcgg ttttgcccgg ctacacgcat ttgcagcgcg cgcagccggt
     4441 tctctgggcg cattggtgtc tggcgtattt tgaaatgctc gaccgcgacc gcgaaagatt
     4501 ggacgaagtc tggcggcgcg tcaacgtttt gccgctcggc tcggcggcgc tggctgggac
     4561 aagttacgaa atcgaccgcg aggcggtagc cagagagctc ggttttgaag gcgtcacggc
     4621 aaacagtctg gacgccgttt ccgaccgcga tttctgcgtc gaatttgtcg gcgcctgctc
     4681 gctcctgatg gttcatctgt cgcgcctggc ggaagatttg attctttacg cgtcgagcga
     4741 gtttcggttc gtcgaattga gcgacgccgt ctcgaccggc tccagcctga tgccgcaaaa
     4801 gaaaaacccg gacgcgctgg aattgattcg cggcaaagcc ggacgcattt tcgggcatca
     4861 aaccgcgctt ttgacgacac tgaaaggctt gccgctggct tacaacaagg atttacagga
     4921 agacaaggaa gcggttttcg atacgattga taacgtcaaa atgtgtctga aagtcgcctg
     4981 catcgttttg aaaaatctgc gcctcgacga ggaaagaacg cggctggcgg cgacgcaggg
     5041 ctatctgaac gcgaccgagc tggctgatta tatggtgcag aaaaacgtgc cattccgcac
     5101 ggcgcacgat ttgacgggaa aaatcgtgct ttacgccatc agcgtcggca gggaattaaa
     5161 cgatttgacg cttgccgaga tgcagaaatt taccgctttt atcgaaaaag acgttttccg
     5221 cgccctgagc ctcgaacaaa ccctgagcag caaatcgcaa atcggcggaa ccgcgcccga
     5281 aagagttttc gaggcgct
//
LOCUS       DASWNE010000116         3795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_604434, whole genome shotgun sequence.
ACCESSION   DASWNE010000116 DASWNE010000000
VERSION     DASWNE010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604434"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..1512)
                     /locus_tag="VGB68_02440"
     CDS             complement(148..1512)
                     /locus_tag="VGB68_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048116.1"
                     /translation="MAKNKKTNKPAQNEKPIRVRMYRVGFGDCFLMSFPVGQDEYRHF
                     VVDCGVHGKGNIGTIERAVDNIMEETGGKIAAIIATHSHQDHISGFSEKFCQFEIGEV
                     WMPWCENPKDELAVKWGKKQAALTSQLEQHFAAQAKTGAAEASTPSREKALAALINLS
                     SSEDSMNLLVNHFKGGDANLLGAFARLTSNAKALHLLKSGFNVGAEVRYLEAGNNLKD
                     AAGVAGLEVRVLSPPRDEKFLAKMDPPKDQGYLRMDDTGAVVEENKLVPFTGKWTIDY
                     QKTSAVAPLTAEEESLLQSQLSDTALNGLAFALDSAKNNTSLVTLFIYRGQYLLFPGD
                     AQYGNWKYWLDKEADEILPRINFLKVAHHGSHNATPKEALEKMATGKFAAMVSTQSTP
                     WDSIPRVPLMTRLSEQTAQKVVRSDWIKLENAPDPSDDAMPPMPVEDPQGFKKGDFWY
                     DYEI"
     gene            complement(1514..3346)
                     /locus_tag="VGB68_02445"
     CDS             complement(1514..3346)
                     /locus_tag="VGB68_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048117.1"
                     /translation="MSNGQFYDEPSSRPLKVYAFDPSRGRTLGNYMTVNVPYEKLENG
                     LSGEYLQVIDYDASNKCYYQPVDLNNPSVLMQGGLEPSESDPRFHQQMVYAVASKTIK
                     IFEFALGRKIKWGFNRNQKIKLPLRIYPHGMQEANAFYSRDLKALVFGYFPASEDDPV
                     SNLPGQTIFTCLSHDIIAHETTHALIDGQRMHFMEATSPDTLAFHEGFADIVALFQHF
                     SFKEVLLDVIKKTGGLIYRPNIASGIETAEGEEAIAGELSTSNPLVQLAKQFGEAMGQ
                     RAALREALGTKPNSKDIEILVEPHARGAILVAAVFDAFFTIYINRIRDLLRIAGVDGK
                     ISTGNELHPDLAARLAAEASKTAEHFLNISIRALDYCPPVDIRFGEFLRAMITADYDL
                     VSDDPRGYRAALIDAFRLRGIRPSEVISYAEESLRWCPPEDLSGKSLICEGLSFNPFK
                     PTSEQKSTNAVCLHAFADKYRKELGLDAERPIRVHSFHTINRVGPDGRLKLEAVAELI
                     QEKEVLVDPNDETLGKMTFYGGVTLIIDLSPEREGEIRYAVRKNLNLDDAQDERLQLQ
                     RSFFKDSAVRYSLNVYAKEISTNLPTKSSEDRKGLSFRMVHLGY"
BASE COUNT          858 a          984 c          972 g          981 t
ORIGIN      
        1 cggtttcaat aataaataaa aaggcttcgt cacattttag agcgaagcct ttttatttat
       61 agttctttta atgcgttttc catgcccggt attactatag cccgtgatta aagggctgga
      121 aatcaggtga ggcaggcgca gaacggatta aatctcatag tcataccaga aatcgccctt
      181 tttaaatcct tgcgggtctt cgaccggcat cggcggcatt gcgtcgtcgg atgggtcggg
      241 cgcgttttcg agcttaatcc agtcgctgcg aacgactttt tgggcggttt gttcgctcag
      301 gcgagtcatc agcgggacgc gcggaatcga gtcccacggc gtgctctgcg tcgagaccat
      361 cgcggcaaat ttgccggtcg ccattttttc aagagcttct ttcggcgtcg cgttatggct
      421 gccgtgatgc gcgactttga gaaaattgat gcgcggcagg atttcgtcgg cttccttgtc
      481 gagccagtat ttccagtttc cgtattgcgc atcgccggga aacagcaggt attgcccgcg
      541 gtagatgaat aatgtcacca ggctcgtgtt atttttggct gaatccagag caaaagccaa
      601 accgttcaga gcggtgtcgg aaagctggct ttgcagcagg ctttcttcct cggcggtcag
      661 cggcgcgacg gcggaagtct tctggtaatc aatcgtccat tttccggtga agggaacgag
      721 tttattctct tcgacgaccg cgcccgtgtc gtccattcgc aaatatccct ggtctttggg
      781 cggatccatt ttagccagaa atttttcgtc gcgcggcggg cttaggacgc gaacctccaa
      841 tcccgccacg ccggcggcat ctttcaaatt atttccggct tccagatagc gaacctcggc
      901 tccgacgtta aagccgcttt tcagaaggtg cagcgccttg gcgtttgacg tcagccgggc
      961 aaaagcgccg agcagattag cgtcgccgcc tttaaaatga ttgaccagca aattcatcga
     1021 gtcttcgctc gagctcagat taatcaatgc ggcgagagct ttctcgcggc tcggcgtcga
     1081 agcttcggca gcgcctgttt ttgcctgcgc cgcaaaatgc tgttccaatt ggctggtcag
     1141 cgcggcttgt tttttgcccc atttgacggc tagctcgtct ttcggatttt cgcaccacgg
     1201 catccagact tcgccgattt caaattggca gaatttctcg ctgaagccgg agatgtggtc
     1261 ttgatgcgaa tgcgtggcga taatcgcggc aattttcccg ccggtttctt ccataatatt
     1321 atcgaccgcg cgctcgatag tgccgatgtt gcccttgccg tgcacgccgc aatcgacgac
     1381 aaaatgccga tattcatcct gaccgaccgg aaacgacatt aaaaagcaat cgccgaatcc
     1441 gacgcggtac atcctgaccc taatcggttt ttcattttgc gccggtttgt ttgttttttt
     1501 attttttgcc attttaataa cccaggtgaa ccattctgaa acttaaacct tttctatctt
     1561 cgctcgattt agtcggcaga ttggtgctaa tctctttcgc gtaaacattt aagctataac
     1621 gaaccgccga atctttaaaa aagctgcgct gcagctgtaa tctttcatcc tgcgcgtcgt
     1681 ccagattcag atttttgcgg acggcgtaac gaatttcgcc ttcgcgttcg ggagaaaggt
     1741 cgataattaa agttacgccg ccgtagaaag tcattttgcc cagcgtttcg tcgttcgggt
     1801 caaccagaac ttccttttcc tgaatcaatt cggcgacggc ttccaatttc agccgcccgt
     1861 cgggtccgac gcggttaatc gtatgaaagg aatgaacgcg aataggtctt tcggcgtcga
     1921 gccccaattc ttttctgtat ttatcggcaa aagcgtgcag gcagacggcg ttggtcgatt
     1981 tttgctcgct ggtcggcttg aacgggttga aactcaatcc ctcgcaaatc agggatttac
     2041 cgcttaaatc ttcgggcggg caccagcgca gcgattcttc cgcatatgaa atcacctccg
     2101 aaggacgaat gccgcgcagg cgaaaagcgt caatcagagc ggctcgatag ccgcgcgggt
     2161 cgtcgctgac caaatcgtaa tcggcggtta tcatcgcgcg cagaaactcg ccgaaacgaa
     2221 tatcgaccgg cgggcagtaa tccagcgcgc ggatggaaat attcagaaaa tgctccgccg
     2281 ttttgctggc ttccgccgcc agtcgcgcag ccaaatcggg atgcagctcg ttgccggtcg
     2341 agatttttcc gtcaactccg gcgatgcgca gcaaatcgcg gatgcggttg atatagatgg
     2401 taaaaaaagc atcgaacacc gccgccacca gtatcgcgcc gcgcgcgtgc ggctcgacca
     2461 gaatctcgat gtctttagaa ttcggcttgg ttccgagcgc ctcgcgcagg gcggcgcgct
     2521 gtcccatcgc ctcgccgaac tgtttggcta gctgcaccag cggattcgag gtcgaaagct
     2581 ctccggcaat cgcttcctcg ccctcggcgg tctcgatgcc ggaagcgata ttcgggcgat
     2641 aaatcaagcc gccggttttt ttaatgacgt cgagcagcac ttccttaaag ctgaaatgct
     2701 gaaacagggc gacgatgtcg gcaaaacctt cgtgaaaagc gagcgtgtcg ggactggtcg
     2761 cttccataaa atgcattctt tgcccgtcta tcagcgcgtg cgtcgtctcg tgagcgataa
     2821 tgtcgtgcga caggcacgta aaaatcgtct gaccgggcag attggagacg ggatcgtctt
     2881 cggaagccgg aaaataaccg aagaccagag cctttaaatc gcggctgtag aaggcgttgg
     2941 cttcctgcat cccgtgcggg taaattcgca ggggcagctt gattttctgg ttgcggttaa
     3001 agccccattt tatttttctg ccgagcgcaa actcgaaaat ttttatcgtt ttgctggcga
     3061 ccgcataaac catctgctgg tgaaatcgcg ggtcggattc gctcggctcc aagccgccct
     3121 gcatcaaaac cgacggattg tttaaatcga ccggctggta atagcattta ttgctcgcgt
     3181 cgtaatcgat tacttgcaga tactcgcccg aaagaccgtt ttccagcttt tcataaggca
     3241 cattgacggt catataattg ccgagcgtgc gaccgcgcga agggtcaaag gcgtaaacct
     3301 tcagcgggcg gctgctcggc tcgtcataaa attgtccatt actcatagag ctattcctta
     3361 atcgttttag aaattacgcg accagaatga ttttcctcaa tccccgattt aaaaagccga
     3421 aaatcaagcc tgcaatgaaa atttccatgt tgacggcggc atcggcgagc ggaaaacatc
     3481 ggcgtcggcg agccgttaaa aagccgtaga tttaataatc aaatccgaat gtgggagaat
     3541 taaaatctat ttaaaaagtt ttgtcaacaa aatttttaga aatctttccg gcaataaaat
     3601 tccgtccgcg ccgcgccgcc cggcatatgt tttagagata aaaaacaagc tctgaaatat
     3661 aatgaaaacc ggcgcggttt gccgacggaa aatttattta gtcggctgcc gctttgtaaa
     3721 ttcgctgccg cttcatttgc aaataatcgc atcgctgctt ttcctcacgc gccgatttct
     3781 gtagtatatt ggcat
//
LOCUS       DASWNE010000117         2540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_604468, whole genome shotgun sequence.
ACCESSION   DASWNE010000117 DASWNE010000000
VERSION     DASWNE010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604468"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="VGB68_02450"
     CDS             complement(<1..776)
                     /locus_tag="VGB68_02450"
                     /inference="COORDINATES: protein motif:HMM:NF019371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HEY0048118.1"
                     /translation="MRYAKPPLNLDGQIALLRSRGLLVSDVDKAKNYLSNISYYRLSA
                     YMLPLKEPGSDNFLPDTDFQDVLNLYLFDREFRILVFDAIERLEIAFRTQIIYHPSLV
                     GGAFWFEDGTYFDDPYRQADHLQKIDDEVYRATEVFKDHFFSKYDEHARMPAWMTFEV
                     VSLGLLSKIYRNLKMSSAKKDISKHFGLPQPYILESWMQSITYVRNVCAHHSRLWNRV
                     LTLRPQRLKKPNNLWISDSPPNDKMYYFLCCILYLLRSINP"
     gene            complement(1535..1804)
                     /locus_tag="VGB68_02455"
     CDS             complement(1535..1804)
                     /locus_tag="VGB68_02455"
                     /inference="COORDINATES: protein motif:HMM:NF021556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2280 domain-containing protein"
                     /protein_id="HEY0048119.1"
                     /translation="MAKKRLERDVKEFIVRSLAEYEKLPDVVTAVKEKFGVIIRPQTA
                     QLYKPDTVAGKDLSAPLKTLFYETRRKYNEKLEAEFREWRKNRSS"
     gene            complement(1878..2246)
                     /locus_tag="VGB68_02460"
     CDS             complement(1878..2246)
                     /locus_tag="VGB68_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048120.1"
                     /translation="MKADEFNQDFELDKKARRGLRAVPKKPVGFPEVLTADGEPFDAN
                     QTYFYFDEQARTVRQTDGLRRVDAAWLGSLGFRVVLIEKLRSDRNAALDDGQAFIRNQ
                     IADFQKIIESFELEKTLEEI"
     gene            complement(2243..>2540)
                     /locus_tag="VGB68_02465"
     CDS             complement(2243..>2540)
                     /locus_tag="VGB68_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048121.1"
                     /translation="GASFEVGANDFQITGVHLLTASDSEFLRINKDAILYVLQQAFLN
                     ETLFKHSAQLLADFQTEVFELQARIDDGEESSEVSFEAAREIIKAWFVELLRQI"
BASE COUNT          705 a          566 c          533 g          736 t
ORIGIN      
        1 ggatttattg agcggagcag atacagaata caacaaagaa aataatacat tttgtcattc
       61 ggaggagaat cggaaatcca cagattatta ggttttttga gtctttgcgg tcttagagtc
      121 agaacccgat tccaaagcct gctgtgatgg gcgcaaacat ttctcacata ggtaatactt
      181 tgcatccagc tttcgagaat gtaaggctgc ggcaatccga aatgttttga aatatctttc
      241 ttggctgacg acattttcag attcctgtaa attttggaca aaagacccag agaaacaact
      301 tcaaaagtca tccaagccgg cattcttgcg tgttcgtcgt atttgctgaa aaaatggtct
      361 ttaaaaactt ccgtcgctcg atatacttca tcatcaatct tttgaaggtg gtcagcttga
      421 cgataagggt cgtcgaaata tgttccgtct tcaaaccaaa aagcgccacc gacaagggaa
      481 ggatgataaa taatctgagt gcgaaaagcg atttcaaggc gctcaatcgc atcaaagacc
      541 aatattctaa actccctgtc aaaaagataa agatttaaaa cgtcctgaaa atcagtgtcc
      601 ggaagaaaat tatcacttcc gggttctttc aagggcagca tataggcgct tagtctgtag
      661 tagctgatgt tagagaggta gtttttggct ttgtcaacat cagagacaag aagcccccgg
      721 cttcttagaa gcgcgatttg tccatccaag ttaagcggag gcttagcata cctcataaag
      781 cttcggaaaa gagaaaaccc gcccgtttga gcttacctta cggcataggc ttggcaggta
      841 ttattaaatt aattttacgg cactttgtgc cattttgcaa tcaatttgat accaaacttg
      901 tgaaaaaaga tgcaaactaa tttggttttc gacgataaaa ttgtttgata tgccatacaa
      961 tttgttctta aactcatcat tttgagaggt tcagcagtaa gctatgtgtt attaattatt
     1021 ttcgatttct ggaaacaatt tacgaataat aatttcttat tggggaagtt gacaaaaggc
     1081 gagagcgaac gcggacagtc taggaaatta aacgggtaag ctctgttgtc aacaaatata
     1141 ggcgagggcg agcgcgggcg gcttgagaaa ttgaacgcgg gctgccctca aactcttcta
     1201 aatgccatac cctttgttac attcctatgg tgttacattt ttcaattcta aaatgccagg
     1261 tttcgtgagg tttttagctg ttaaatttta cttatacggg cttttgctga aaatcatatt
     1321 aagctgcttt ttatcttaga cttaacaaat ttgcggtttt gattccgtat aagtcccgta
     1381 taagtaaatc ggtaaaatct tcggctaaaa tcttccttta ccggctcgcc gccgaaaacc
     1441 ttcaaaatca ccgtaaacgg ctgaaaactc ttaatttgtt cgcgcgcctg tgatttcaca
     1501 tttcttgatt tactaaagga aaataagcga aagtctagga agaacggttt ttgcgccact
     1561 cccgaaattc tgcttctaac ttttcgttgt attttcgccg cgtctcgtaa aaaagcgttt
     1621 ttaacggcgc gctcaaatcc tttccggcaa ccgtatccgg cttgtagagc tgcgcggttt
     1681 gtgggcggat aatgactccg aatttctcct ttaccgcggt gaccacatcg ggcagctttt
     1741 catactccgc taaacttcta acgataaatt ctttaacgtc tctttcaagt ctctttttcg
     1801 ccatacaaaa acctcatttt cgtaaaggac ggttaaggac agtctaggaa aaccgccgcc
     1861 agccgaccgc cgccaattta tatttcttcc agcgtttttt cgagttcaaa actttcaata
     1921 attttctgaa aatcggcaat ctgatttcta atgaaagcct gtccatcgtc gagcgccgcg
     1981 tttctatccg agcgaagttt ttcaatcaag acgacgcgga aaccgagcga gccgagccaa
     2041 gccgcatcaa cgcgccgcaa gccgtccgtc tggcgaactg tgcgcgcctg ttcgtcaaag
     2101 tagaaatatg tttgattcgc gtcgaatggc tcgccgtcgg cggtcaagac ttctgggaag
     2161 ccaacaggct ttttcggaac ggctcgaagc ccgcgccgcg cttttttgtc cagttcaaaa
     2221 tcttgattaa attcgtctgc tttcatattt gccttaataa ctccacaaac cacgccttta
     2281 tgatttcccg cgccgcttca aaacttactt cgctcgattc ttcgccgtcg tcgattctgg
     2341 cttgaagctc gaaaacttcg gtctgaaaat cggctaacaa ttgagccgaa tgtttgaaaa
     2401 gcgtctcgtt taaaaacgcc tgctgcaaaa cgtagagaat cgcgtctttg ttgattctca
     2461 gaaactccga atccgaggcg gtcaacagat gcacgccggt gatttgaaaa tcattcgcgc
     2521 cgacttcaaa gctcgcgccg
//
LOCUS       DASWNE010000118         2586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_356361, whole genome shotgun sequence.
ACCESSION   DASWNE010000118 DASWNE010000000
VERSION     DASWNE010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356361"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VGB68_02470"
     CDS             <1..209
                     /locus_tag="VGB68_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048122.1"
                     /translation="GLMAKLLGIIKVGEIDGKTISLQQWIFNNIFLSIAAPINASLMY
                     AVSFILVWLLLMWILYRKGIFLKI"
     gene            complement(309..2531)
                     /locus_tag="VGB68_02475"
     CDS             complement(309..2531)
                     /locus_tag="VGB68_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048123.1"
                     /translation="MKTKLLLPFVLVCLISASSFGQMRNVRTVKPKAIAPSPQFNNQN
                     QLPIRRVILYSNGVAYIERRGLVSGNAEINLSFKQSQVDDVLKSMLVLDLQGKIGAVS
                     YNSSMPTGARMSEIPFSVNPLTADGGGIAGVLAQLQGAKVSVTTTKGTFAGAILTVER
                     KEVRGEKSTTVSPFLVIASDAGEISTFDLNEIRSVKLLDEGTRRDLSEFANASASARR
                     RDAKTISVTSEGTGQREMIVSYTIAAPIWKTTYRVVLDEAGKPFFQGWAIVDNISDED
                     WQDVQLSLVSGTPISFIQPIQQPFYRYRPVIPIPQDLQVQPQVYEPQSMITDGTETKI
                     QTSINSRQAENLPKGTNYSSLQKISPSTRTESSSNNYQIDGSSGTENSVVVTSESAQT
                     ISISDALTSQKSGVEVNATGGEIGDLFEYRIDRPVSVLRDRSALIPIVQTKMDGERVA
                     IYNEAVRQDRPYSGVLLKNLTSLTLENGSLTVIDGNAYAGEALMERLKPKEQRLISFA
                     LDLGTHVRVRPVQNREAVRLIKIVNGVFQIHYFTSNEKLYQITNQTDRPKVLYIEYAI
                     RDGWQLSENTPKPEYSTQRYHRFRVELKPLEEKGLTIAERQPLMETYSLTSLTRAQLD
                     LFLSQRYIDAATKAKLEKLIDLRGQIAQIDARLEAFESEEESISTDQKRLRENIEALS
                     KTPEAKQLITRYIQKADEQESRLEQINKERKSLQQERARIESELATEIRSFDLERKPN
                     "
BASE COUNT          544 a          687 c          670 g          685 t
ORIGIN      
        1 cgggcttgat ggcgaagctt ctgggcatta tcaaggtcgg cgaaattgac ggcaaaacga
       61 tttcattgca gcagtggatt ttcaacaata tttttctttc aatcgccgcg ccgataaacg
      121 cctcgctgat gtatgccgtc tcgtttattc tggtctggct gctgctgatg tggattttgt
      181 accgaaaggg aattttcctc aaaatttgat aaaagaacga tgaatgatga acgcggaatg
      241 atgaattgca atttctgcgt ttcattattc ttaattcatc gtttatcgtt ccgggttcat
      301 cgtttctttt agttcggctt gcgctcgagg tcgaagctgc gaatctccgt cgccagctcg
      361 ctttcgatgc gcgctctttc ctgttgcaat gattttcttt ccttgtttat ctgttcgagc
      421 ctcgattcct gctcgtcggc tttttgaata taacgcgtga ttaactgttt ggcttcgggc
      481 gttttcgaca gcgcctcgat gttttcgcga agccgtttct ggtcggtcga gatgctttcc
      541 tcttcgcttt caaaggcttc gagccgggcg tctatctgcg cgatttgacc gcgcaaatca
      601 atcagctttt cgagcttcgc tttcgtcgcg gcgtcgatat aacgctgcga gagaaacaag
      661 tccagctgcg ctctggtcag cgaggtcagg ctgtaagttt ccatcagcgg ctggcgctcg
      721 gcgatagtga gtcccttttc ttccagcggt tttaattcga cgcggaagcg atgataacgc
      781 tgcgtcgaat attccggctt cggcgtgttt tccgaaagct gccagccgtc gcgaatcgcg
      841 tattcgatgt ataaaacctt cggtcggtcg gtctggttgg taatctggta gagcttttcg
      901 ttgctcgtaa aataatgaat ctggaaaacg ccgttgacga tttttatcaa ccggacggct
      961 tcgcggtttt ggacgggtcg gacgcggacg tgcgttccca aatccagcgc gaaggaaatc
     1021 aggcgctgct ctttcggctt gagcctttcc atcaaagctt cgcccgcgta agcgttgccg
     1081 tcaatcacgg tcaggctgcc gttttcgagc gtcaggctgg tcagattttt caataaaacg
     1141 ccgctgtagg gtcggtcttg cctgacggct tcgttgtaaa tcgcgacgcg ctcgccgtcc
     1201 attttcgtct ggacgatcgg aatcagtgcc gagcggtcgc gcaaaaccga cacggggcgg
     1261 tcgatccggt attcgaacaa atcgccgatt tcgccgcccg tcgcgtttac ctcgacgccc
     1321 gatttttgac tggtcaaagc atcgctgatg gaaatcgtct gagcgctctc gctggtgacc
     1381 acaacactgt tttccgtgcc gctcgaaccg tcgatttgat agttattcga actggattcg
     1441 gttcgggtcg agggcgagat tttctgcagg ctgctgtaat tcgttccttt cggcagattc
     1501 tccgcttgtc gggaattaat actcgtttga attttagttt cggttccgtc cgttatcatg
     1561 ctctgcggct cgtaaacctg cggctgtacc tgcaaatcct gcggaatcgg aatgaccgga
     1621 cgatagcgat aaaacggctg ctgaatcggc tggataaacg aaatcggcgt gccggaaacg
     1681 agcgaaagct gaacgtcctg ccagtcttcg tcgctgatgt tgtcgacaat cgcccagccc
     1741 tgaaaaaacg gttttcccgc ttcgtccagc acgactcggt aagtcgtttt ccaaatcggc
     1801 gcggcgatgg tgtagctgac gatcatctcg cgctgccccg tgccttcgct cgtcacggaa
     1861 atcgtcttgg cgtcgcgccg ccgcgccgaa gccgaagcgt tggcgaactc cgacaaatcg
     1921 cgccgcgttc cttcgtcgag cagcttgacg gagcgaatct cgtttaaatc aaaagtcgaa
     1981 atctcgcccg cgtccgaagc gataacgaga aacggcgaaa cggtcgtcga tttttcgccg
     2041 cgcacctctt ttctttcgac cgtcagaatc gcgcccgcaa aagttccttt cgtcgtcgtg
     2101 acggaaactt tagcgccctg caattgcgcc agaacgcccg cgattccgcc gccgtccgcc
     2161 gtcagaggat tgaccgaaaa gggaatttcc gacatccgcg cgccggtcgg catcgacgaa
     2221 ttataagaaa ccgcgccgat ttttccctgc aaatccagca ccagcatcga ctttaaaacg
     2281 tcgtccacct gcgattgctt aaacgaaaga tttatctccg cattgccgga aacaagcccg
     2341 cgccgctcga tataagctac gccgttggaa taaagaatca cgcgccgaat cggcagctgg
     2401 ttctgattat taaactgcgg cgacggcgca atcgctttcg gcttaacggt tctgacgttg
     2461 cgcatctgcc cgaaactgct cgcggaaatc aggcacacca atacaaaggg aagcaaaagt
     2521 tttgttttca taaacggaaa tccttaaatg gaaattttaa aaaattgaca gcaacatttc
     2581 gcgaga
//
LOCUS       DASWNE010000119         1965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_674564, whole genome shotgun sequence.
ACCESSION   DASWNE010000119 DASWNE010000000
VERSION     DASWNE010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674564"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..867
                     /locus_tag="VGB68_02480"
     CDS             49..867
                     /locus_tag="VGB68_02480"
                     /inference="COORDINATES: protein motif:HMM:NF020442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HEY0048124.1"
                     /translation="MPYENITIESWRQFQEIVAEPKFRSWAFRGNASADWQLFSTLSR
                     YLKTYGIHKDAWTRQEYRILRIFRRKTHLLLEHIPDEDDSFQWLALMQHHGAPTRLLD
                     FTWSPYVAAFFALERATTDAAVWALFPPKLSGAKIRTIRASQKVEAGEPGPWVKGNYE
                     KYFLPNEQKFVIIGEPHRMNQRLIAQSGTFVMPGVLDIPIEELVPKGAIVKLTLVTEK
                     LRKQAMAELYKMNISNATLFPGLDGLARSLAYELEFHWAFDPTTMERYEGYFIE"
     gene            complement(934..>1965)
                     /locus_tag="VGB68_02485"
     CDS             complement(934..>1965)
                     /locus_tag="VGB68_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEY0048125.1"
                     /translation="FKGIIYDTASVSTLHAIAVAREKLNVRIREDGMSGRDDLPLLRV
                     YCSEHVHSSIDKAVILLGLGQKSLRKIPTGECFEMLPEKLAEAVEEDRQNGFLPFCVV
                     ATVGTTSSSSIDPVEKIADVCEKHNLWLHVDTAYAGSAAIVPEFQAHFKGINRADSIV
                     TNPHKWLFTPFDLSVLYVRDLDLLKKTFSLVAEYLKVTETVINQMDYGIQLGRRFRSL
                     KLWFVMRYFGQQGLIERLREHCRLAALFASRIEESRDWELLAPVPFALVCFRAAPEGV
                     AEAELDALNEKIMNDINASGAAYLSHTKLNGKFTLRLSVGSIRVEERHIEKIWNLLNV
                     NLQEIFNAN"
BASE COUNT          506 a          479 c          505 g          475 t
ORIGIN      
        1 ataatgcgag tctgaattat cagattgcag cgctcataaa cagctgatat gccatatgaa
       61 aatattacaa tcgaatcctg gcggcaattt caagagatag ttgcggaacc gaaattcagg
      121 agctgggcgt ttcgcggaaa cgcgtctgcc gattggcagc ttttcagcac attgtcgcgc
      181 tatttaaaaa cttacggtat tcataaagat gcctggactc ggcaggagta tcgcattctc
      241 agaatatttc gccgcaaaac tcatcttctc ctggaacata ttcccgacga ggacgactcg
      301 tttcagtggc tggctctgat gcagcatcac ggcgcgccga caagattact tgattttacc
      361 tggtcacctt atgtggcggc tttttttgct ctcgagcggg cgacgacaga cgcggcggtc
      421 tgggctttgt ttccgcccaa gctcagcggg gcaaaaatca gaacgattcg cgccagtcag
      481 aaagtggagg ctggcgagcc cggtccgtgg gtaaaaggca attatgagaa atatttcctg
      541 ccgaatgaac aaaaatttgt aattatcggc gagcctcatc ggatgaacca gcgcctgata
      601 gctcaatcgg gcacttttgt aatgccgggc gttctcgata ttccgattga agaacttgtt
      661 ccgaaaggcg caattgttaa gcttacgctg gtcacggaaa aactcaggaa gcaggcaatg
      721 gcggaactgt ataagatgaa tatcagcaat gcgacattgt ttccggggct cgacggatta
      781 gcccgctcgc ttgcatatga actggagttt cattgggcat ttgatccgac gacgatggaa
      841 aggtacgaag gatactttat agaatgatta agacttataa tatctgaaaa acaattcaat
      901 gcttgaaaaa aaagtccgat aaaataaatt cctctagttt gcgttaaaaa tctcctgcaa
      961 atttacattc agcaaattcc agattttttc aatgtgtctt tcttcgacgc gaatgcttcc
     1021 gaccgaaagc cgcagcgtaa atttgccgtt cagcttcgta tgcgaaagat aagccgcgcc
     1081 ggaagcgttg atgtcgttca ttattttttc attcagcgcg tcgagctccg cttcggcgac
     1141 gccttcgggc gcggcgcgaa agcagaccag cgcgaacgga acgggcgcga gcaattccca
     1201 atcgcggctt tcctcgatcc gcgaggcgaa aagcgcggcg agccggcaat gctctcttaa
     1261 tctttcaatc aagccctgct gaccgaaata gcgcatcacg aaccacaatt tcagcgagcg
     1321 gaagcggcga ccgagctgga tgccgtagtc catttgatta atcacggttt ccgtcacttt
     1381 caaatattcg gcgacgagcg agaacgtttt tttgagcaag tccaaatccc tgacatacaa
     1441 aaccgacaaa tcaaacggcg tgaaaagcca tttgtgcggg ttcgtgacga tcgaatcggc
     1501 gcgattgata cctttgaaat gcgcctgaaa ttcgggcaca atcgccgccg agccggcgta
     1561 agccgtatcg acgtgcagcc agagattatg tttttcgcaa acgtcggcga ttttttcgac
     1621 cgggtcgatg ctggagctgg aagtcgtgcc gacggtcgcg acgacgcaga acggcagaaa
     1681 accgttttgc cggtcttctt cgaccgcttc cgccagtttt tcgggaagca tctcaaagca
     1741 ttcgccggtc gggatttttc gcagcgattt ttgtccgagc ccgagcagaa tgacggcttt
     1801 gtcaatcgaa gaatgaacgt gctcggagca gtaaacgcgc aaaagcggca aatcatcgcg
     1861 accgctcatt ccgtcttcgc ggatgcgcac gttcagtttt tcgcgcgcga cggcgatggc
     1921 gtgcaaggtg gaaaccgaag ccgtatcgta aataattcct ttaaa
//
LOCUS       DASWNE010000120         1848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_251627, whole genome shotgun sequence.
ACCESSION   DASWNE010000120 DASWNE010000000
VERSION     DASWNE010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251627"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..723)
                     /locus_tag="VGB68_02490"
     CDS             complement(202..723)
                     /locus_tag="VGB68_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048126.1"
                     /translation="MKLLCIFFLTLFFVLSCAAQETCGLEAKEAPALLGLKLGIAPAQ
                     AQGVFGKDLKIKNKRKGEYTFFQNFIEKPPPNSLAGVRALYLRFFDGSLYQIEVFYEE
                     KSQLQTLEAFTAMLSSKWNFPVSLWITAQGKARIDCGELTLVADNVLNPRIEITNESD
                     RARVEARRKRKND"
     gene            1112..1789
                     /locus_tag="VGB68_02495"
     CDS             1112..1789
                     /locus_tag="VGB68_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048127.1"
                     /translation="MKIKILGERNLVIACENLARTDSDLAFIFQTYGTPPLWQREPNF
                     ATLVHIILEQQVSLASALSAFNKLKEKLGEITPEAVLNLSDEEMKAAYFSRQKTVYAR
                     ELARAILEGNLDLKSLANLPDEKAKHELKKIKGIGDWTSDIYLLMAMLRADVMPKGDL
                     ALHIAWKKLKNLEQAPNSDEFQQIAEKWKPLRAVAARLLWHFYLSQRKASAAKGREKA
                     GKKNLQQ"
BASE COUNT          512 a          434 c          426 g          476 t
ORIGIN      
        1 gattttatat gatgtttatt attgacggca acggcgtccc gtccgtcgcc aaaatggtta
       61 agctgtcgta gacgaattca agtgtaattt ccctgtttaa aaggcaagta aatcttgcct
      121 tttacttttt tgccgcgcta agcttcagtt tagccaatcg gcgcgacacc gagatttaca
      181 taaaaatttg acggttatga tttaatcgtt ttttcgtttt ctccgggctt ccacccgggc
      241 gcggtctgat tcgtttgtta tctcgattcg cggattcaaa acattgtcgg caacgagtgt
      301 gagctcgccg cagtcgatac gggctttgcc ctgcgcggta atccataaag agacggggaa
      361 attccatttc gatgaaagca tagcggtaaa agcttcgagc gtttgcaatt gacttttttc
      421 ctcgtaaaaa acctcgatct ggtaaagcga accgtcaaaa aatcttaaat aaagcgcgcg
      481 cacgcccgct aaggaattcg gcggcggctt ttcgataaag ttctggaaaa acgtgtattc
      541 gccctttctt ttatttttga ttttcaaatc ttttccgaaa acgccctgcg cctgcgcggg
      601 cgcgatgccg agttttaaac cgagcaaagc gggagcctct ttcgcctcta atccacaggt
      661 ttcctgcgcc gcgcaggata aaacaaaaaa caacgtcaga aaaaatatgc agagcagttt
      721 catagattaa tataatctta tctcgccggt attttattgc ataattaaat cgaaccgcat
      781 taaaattgcg agttcgatta ttataaaaac aaactgcccg attgtgcctg tatcgagttt
      841 taaaagcgag aagtgattta tgtttaaacg cgtcgcccgg tttgttttcc ggatttcgct
      901 tttcttttcg gctgcctccg gctcaaacgg atgtaacccg caaaggtcaa tctgcggagg
      961 ctaatccggc gactgattta tccgcaggcg tagaaagcct cgcgtttgat aaaaattcag
     1021 cccgggcata tcgcccgcgc aatcaggact tttatacaaa agacgaatta attgtaaaag
     1081 tcggaaccgc tcccaaaaac aaagacgaaa tatgaaaatt aaaatcctcg gcgagcgaaa
     1141 tctcgtcatc gcctgcgaga atctcgcccg aaccgattcc gatttagcct ttattttcca
     1201 gacctacggc acgccgccgc tctggcagcg cgagccgaat tttgccacgc tcgttcatat
     1261 cattctggaa cagcaggttt cgctcgcctc ggctttatcg gcgtttaata aattgaaaga
     1321 aaagctcgga gaaattacgc cggaagccgt tttgaatttg tcggacgaag aaatgaaagc
     1381 ggcttacttc agccgccaga aaacggttta cgcgcgcgag ctggcgcgag cgattctgga
     1441 aggaaatctg gatttgaaat cactcgcaaa cctgccggac gaaaaagcaa aacacgagct
     1501 taaaaagata aaaggcatcg gcgattggac ttcggacatt tatcttttga tggcgatgct
     1561 gcgcgcggac gtaatgccga aaggcgattt ggcgctgcac atcgcgtgga agaaattaaa
     1621 aaatctggaa caggcgccga attccgacga gtttcagcaa atcgccgaaa aatggaaacc
     1681 gcttcgcgcc gttgccgcgc gcctgctctg gcatttttat ctcagccaga gaaaagcttc
     1741 agccgcgaaa ggacgcgaaa aagccgggaa gaagaattta cagcaataga ctggagatga
     1801 tttaaataaa aagcatctgc ctcattcctg acaatctctg ctattcct
//
LOCUS       DASWNE010000121         1921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_39415, whole genome shotgun sequence.
ACCESSION   DASWNE010000121 DASWNE010000000
VERSION     DASWNE010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39415"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VGB68_02500"
     CDS             complement(<1..257)
                     /locus_tag="VGB68_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048128.1"
                     /translation="MKLFKPAIGFFLIFFSFFSNTMNAQTDRFSENYKKFKAFYEKEL
                     KDASIIGSSFVFLKDNKIIAQEFYGAANLEKNQKTDANTIY"
     gene            complement(278..1036)
                     /locus_tag="VGB68_02505"
     CDS             complement(278..1036)
                     /locus_tag="VGB68_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048129.1"
                     /translation="MIPVFLIGGGWRAETFPQTYGRFLKTATRDTERQIAIVVAGETG
                     ADSGEQFLRFRRAFEAVGLNPAEAVHLIVSADNPLTAEKLAAAPPTGIFVCGGLTPAY
                     HEALCFDKSWLDYLTENKIPYGGFSAGASIAAGSAIIGGWRRKINNKIVEIVNENAGE
                     DLDFLDVRRGLGLAPFAVDVHASQWGTLSRLVQAVDAGLVARGVAIDENTMLEISENR
                     ISVSGAGNIYRVRRIGNHSAIDIFQAGNHISFNE"
     gene            1194..>1921
                     /locus_tag="VGB68_02510"
     CDS             1194..>1921
                     /locus_tag="VGB68_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY0048130.1"
                     /translation="MKDAMPKDAAAVILLRENSSEVFLAQRNPKISFLGGFHAFPGGK
                     VDKEDAAIKVRNCADEELAKFIACAVRETFEEVGVLLVRGGDKITKGQRASVLDDLIS
                     GRFSFAEILRMWGLWIDAADFRYAGFWTTPKFSPVRFKTRFFLVVCPRKQEPRVFGEF
                     VEGKFIAPPDALKLWKNSEILISPPVLLTLQALAESGQQSATGSQPETENQSAKTVIE
                     KLLLQSQKYDGKIRCVRVHPSIAC"
BASE COUNT          473 a          488 c          455 g          505 t
ORIGIN      
        1 tgataaatcg tattcgcgtc cgtcttttga tttttttcga gattcgccgc gccgtaaaat
       61 tcctgcgcga taattttgtt gtcttttaag aaaacgaaac tgctcccgat gatgctcgca
      121 tcctttaatt ccttttcgta aaaggcttta aattttttat agttttcgga aaacctgtcg
      181 gtctgcgcat tcatcgtgtt tgaaaaaaaa ctgaaaaaga ttaaaaagaa accaatcgcc
      241 ggtttgaaaa gcttcattta tttaccttta ttttaattta ttcattaaac gaaatatgat
      301 ttcccgcctg aaaaatgtca atcgccgaat gatttccgat tcgccggacg cgatagatat
      361 ttcccgcgcc ggaaacggaa atgcggtttt cggaaatttc cagcatcgta ttttcatcaa
      421 tcgccacgcc ccgggcgaca agaccggcat caaccgcctg aacgagccgc gaaagcgttc
      481 cccactggct ggcgtggaca tcgacggcaa acggagctaa accgagaccg cgccgcacgt
      541 cgagaaaatc caaatcttca ccggcatttt cattcacaat ttcgacgatt ttgttattaa
      601 tctttcgccg ccagccgccg attatcgcgc tcccggcggc aatcgaagcg cccgccgaaa
      661 aaccgccgta cggaatcttg ttttcggtca aataatcgag ccacgattta tcgaagcaaa
      721 gcgcttcgtg ataagcgggc gttaagccgc cgcaaacgaa aattccggtc ggcggcgcgg
      781 cggctaattt ttcggcggtc agcggattat cggcggaaac tattaaatgc accgcttcag
      841 ccggatttaa accgaccgct tcaaacgcgc gccgaaatct cagaaattgc tcgccggagt
      901 ctgcgccggt ttctcccgcg accacgattg caatttgcct ttcggtatct cgcgtcgccg
      961 ttttcagaaa tcttccgtat gtttgcggaa aagtttcggc gcgccagccg ccgccgataa
     1021 gaaaaacggg aatcattttt ttaaacctcg atagctatct ttccacattc ttctcatttt
     1081 cgctcgatcg aattttgacg tttaatttat gttcggatta aagcggcagc ttgtcaaaga
     1141 aatttcgctc ctcgcgctct tgcttctgtg cgcctttgcg ttaaattaaa tatgtgaaag
     1201 atgcaatgcc gaaagacgcc gccgccgtca ttttactgcg cgaaaactcg tcggaagttt
     1261 ttctcgcgca gcgcaatccg aaaatttcgt ttctcggcgg tttccacgct tttcccggcg
     1321 gaaaagtcga taaggaagac gccgctataa aagtcagaaa ttgcgccgac gaagaactcg
     1381 caaaatttat cgcctgcgcc gtgcgcgaga cgtttgaaga agtcggcgtt ttgctggtgc
     1441 gcggcggcga taaaatcaca aaaggtcaac gcgcttccgt gctcgacgat ttaatcagcg
     1501 gcaggttttc tttcgcggaa attctccgga tgtggggttt gtggattgac gcggcagatt
     1561 tccgatacgc gggattttgg acgactccga aattttcgcc cgtgcgattt aagacgagat
     1621 tttttctcgt cgtctgcccg cgcaaacaag agccgcgggt tttcggcgaa ttcgtcgagg
     1681 gcaaatttat cgcgccgccg gacgcgctga agctctggaa aaattcggag attttaattt
     1741 cgccgcccgt gctgctgacg cttcaagctc tggcggaaag cggtcagcaa tcagcaaccg
     1801 gcagtcagcc ggagacggaa aaccaaagcg cgaaaaccgt tattgaaaaa ttgcttttgc
     1861 agtcgcaaaa atacgacggc aaaattcgct gcgtcagagt tcatccgtct attgcttgtt
     1921 t
//
LOCUS       DASWNE010000122         3601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_428067, whole genome shotgun sequence.
ACCESSION   DASWNE010000122 DASWNE010000000
VERSION     DASWNE010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428067"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..794)
                     /locus_tag="VGB68_02515"
     CDS             complement(75..794)
                     /locus_tag="VGB68_02515"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEY0048131.1"
                     /translation="MEALILSLALTTIAAAAFLASKPLEKRILVACALAAAVYIGVDD
                     FVTGLPSLVKGLNLAGAGWNWTGKFLSLALSATVIAALKLSPAAVGLTTGQRHTKTGL
                     VALLLFIVWGSCLGMLFKPGVPDMETLLFQATMPSLSEELVYRGIAPAILLGLFRGQR
                     FDYGMPWVVILSSAVVFGVWHGLKYSNGLFGFDAMSALFPFIGSIPGGWLRYKTGSLL
                     FPILAHSFANVAFHVAGGFAA"
     gene            complement(1022..1097)
                     /locus_tag="VGB68_02520"
     tRNA            complement(1022..1097)
                     /locus_tag="VGB68_02520"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1062..1064),aa:Ala,seq:ggc)
     gene            1208..2239
                     /gene="queA"
                     /locus_tag="VGB68_02525"
     CDS             1208..2239
                     /gene="queA"
                     /locus_tag="VGB68_02525"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEY0048132.1"
                     /translation="MLITDFDYELPEELIAQQPLARRENSRMLVVNRGENSFADDNFA
                     SFPDFLKKDDVLVLNNTKVFPARLLGEKDTKAKIELFLVRELANQIWETLARPARRLK
                     TGTKIFFGEKLAAEVLEKTEAGHCVVRFESVGDFNEVLEQVGQTPLPPYIKREDGNFD
                     KDRERYQTVFAKQRGAVAAPTAGLHFTPEALEKIKDSGVAIVEITLHVGYGTFEPVRV
                     SDLAQHKVMAEQYEISAAAAQILNAAKASGRRIIAIGTTTTRALESSADADGKIQSGK
                     RLADLTITPGYKFKVIDGLLTNFHLPQSSLLVLVATFAGYQLIMKAYEHAVASRYRFY
                     SYGDCMLIL"
     gene            2360..2899
                     /gene="rimI"
                     /locus_tag="VGB68_02530"
     CDS             2360..2899
                     /gene="rimI"
                     /locus_tag="VGB68_02530"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEY0048133.1"
                     /translation="MAVLERIRKLFVPFEAQEEDFEIIVPAPPTDYEIHTLTANRLDE
                     ICRLNRRCFKKGENYNKQTLTYLLSETNTLSYCMTAANNEMVAFVFATGNIDGTGHIT
                     TIGVAPEHRRRGLAQKLLVYAEAAMQRRKISVIRLEVRVSNVAAQSLYRRFGYATVQR
                     LENYYNNGEDGFLMVKSLF"
     gene            2904..3524
                     /gene="thiE"
                     /locus_tag="VGB68_02535"
     CDS             2904..3524
                     /gene="thiE"
                     /locus_tag="VGB68_02535"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEY0048134.1"
                     /translation="MPFTLPKIYPITDAFISRLSHAEQFGRLAEGGARVIQLREKSAS
                     PLAFYESAREVLALAEKINIKVIINDRADIAFALKAGGVHLGQDDLPPQAARRLLGKN
                     AVIGFSTHGVEQAIEAVRLPVDYIAVGPVFATKTKQNPEKTIGLKGVERVRKAVGNFP
                     LVAIGGITLKNAGDVFGAGADSVAVIGDLLGEPDRIIEKMKRFLLL"
BASE COUNT          951 a          911 c          933 g          806 t
ORIGIN      
        1 tatgggacaa attgctcgct ctgcctttag cattctggtt aagccgcgcg cgcggcatcg
       61 gctttagcga gttgtcaggc agcaaatccg cccgcgacgt gaaatgcgac gttagcaaag
      121 ctatgagcga gaatgggaaa gagcaggctg ccggtcttgt accgcagcca gccgccggga
      181 atgctgccga taaacgggaa caaagccgac atagcatcga agccgaacaa cccgtttgag
      241 tatttcagcc cgtgccagac gccgaaaacg acggcgcttg acaagattac gacccacggc
      301 attccgtaat cgaagcgctg cccgcggaac agacccaata gaatcgcggg cgcaataccg
      361 cgatagacga gttcttcaga caaagaaggc atagtcgcct ggaagaggag agtttccata
      421 tcgggaacgc cgggcttgaa aagcattccc agacaactgc cccaaacaat gaataataaa
      481 agcgcgacca gcccggtctt agtgtgacgc tgcccggtgg tgagtcccac tgcggcgggc
      541 gagagtttca gcgccgcgat aacagttgcc gaaagtgcca gactgagaaa cttgccggtc
      601 cagttccagc ctgcaccggc gagattaagt cctttaacta atgacggcag tccggtgacg
      661 aaatcatcta cgccgatata gacggcagca gctaaagcgc aggcgaccag tattcgcttt
      721 tccagcggtt tcgacgccag gaacgccgcg gcagcaatcg tcgtcagagc gagtgataaa
      781 atcaaggctt ccatgcggca cctctgtaat ttttaagata actcaatact tcatctcaaa
      841 agatgaaaat aagaagcgga ctgttaacaa agcggcaggc tcatttcaaa aaaggtcgtt
      901 tttgaggcaa taataaacca gaattaattt ttggcgcgca atggacacac acaacaaaaa
      961 agacaagctg gaatgcctgc cttttaatct ttctaagcta ttagttttat tgaggttaag
     1021 ttggtggaga cgagggggat cgaacccctg acctctgcaa tgccattgca gcgctctccc
     1081 agctgagcta cgtccccgta ttttcaaaac gccgagcctt ccggcgaaaa attaatatac
     1141 cagccgagcc gccaatacgc aaccgttgag catttggttt tatcttgtta gtctgtaatt
     1201 ttcctttatg ttaattaccg atttcgatta cgagttgccg gaggaactca ttgcccaaca
     1261 gccgcttgcg cggcgcgaga attcgcggat gctggtcgtc aatcgcggcg aaaattcttt
     1321 cgccgatgat aatttcgcca gttttccgga ctttctcaaa aaagacgacg tgcttgtttt
     1381 gaataacacg aaggtttttc ccgcgcgcct tctgggcgaa aaagatacga aagcgaaaat
     1441 cgagcttttt ctggttcgcg agctggcaaa tcaaatttgg gaaacgctgg cgcgtccggc
     1501 gcggcgctta aaaaccggca cgaagatttt tttcggagag aagctggcgg ctgaagttct
     1561 ggaaaaaacc gaagcgggtc actgcgtcgt cagatttgaa tccgtcggcg attttaacga
     1621 ggttctggaa caagtcgggc agacgccgct gccgccttat atcaaacgcg aagacggaaa
     1681 tttcgataaa gaccgcgagc gttatcaaac cgtttttgcc aaacagcgcg gagccgtcgc
     1741 cgcgccgacc gccgggcttc atttcacgcc cgaagcgctc gaaaaaatca aagattcggg
     1801 cgtcgcgata gtcgaaatta ccctgcacgt cggttacgga actttcgagc cggttcgcgt
     1861 cagcgatttg gcgcagcaca aggttatggc tgaacaatat gaaatctcgg cggcggcggc
     1921 gcaaatttta aatgcagcga aagcgagcgg gcgaagaatc atcgccatcg gaacgacgac
     1981 gacgcgcgcg ctcgaatctt ccgcagacgc ggacggaaaa attcaaagcg gaaagcgcct
     2041 ggctgatttg acgattacgc cgggttataa atttaaagtg attgacgggc ttttgacgaa
     2101 ttttcacctg ccgcaatctt ccttgctggt tttagttgca actttcgccg gttatcaact
     2161 tataatgaaa gcatacgagc acgccgtcgc gtcgcgttat cgcttttaca gctacggcga
     2221 ttgtatgctg attctttgag tcgcggaaag tcgcaggaag ttgacaggaa gttgcggata
     2281 gttaaagcag tttcaaattt caatcttcga taactttctg caacttcctg caactctgaa
     2341 actctgttaa cttactttta tggcagtttt agaaagaatt cgtaagcttt tcgtgccgtt
     2401 tgaggcgcag gaggaagatt ttgagatcat cgttcccgcg ccgccgaccg attatgaaat
     2461 tcacacgctg actgccaacc ggctcgacga aatctgccgg ctcaaccggc gttgttttaa
     2521 aaaaggcgaa aattacaaca aacaaacgct gacctatctg ctttccgaaa caaatactct
     2581 gagttactgt atgacggcgg cgaataacga aatggtcgcg ttcgtttttg cgaccggtaa
     2641 tatcgacggc acgggacaca tcacgaccat cggagtcgcg cccgaacatc ggcggcgggg
     2701 attggcgcag aagcttctgg tttacgccga agccgcgatg cagcgacgca aaatcagcgt
     2761 catccgcctc gaagtccgcg tcagcaacgt cgccgcgcag agcctctacc ggcgattcgg
     2821 ttacgcgact gtacaaagac tggaaaatta ctataacaac ggcgaagacg gatttttaat
     2881 ggtgaaatca ctgttttaaa tttatgccgt tcacacttcc gaaaatctac ccgattaccg
     2941 acgctttcat cagccgactt tctcacgccg aacagttcgg acgacttgcc gaaggcggcg
     3001 cgcgcgtgat tcaattgcgc gaaaaaagcg cctcgccgct cgcgttttac gaatcggcac
     3061 gggaagttct ggctcttgcc gaaaagataa acataaaagt tattattaac gaccgcgccg
     3121 acatcgcttt tgctttgaaa gccggcggcg ttcatctcgg acaggacgat ttgccgccgc
     3181 aggcagcgcg tcggctgctg gggaaaaatg ccgttatcgg attttccacg cacggcgtcg
     3241 agcaggcaat cgaagccgtc aggcttccgg tcgattacat cgccgtcgga cctgtttttg
     3301 cgacgaaaac aaaacaaaat ccggaaaaga caatcgggct gaaaggcgtc gagcgcgtgc
     3361 ggaaagccgt cggaaatttt ccgctcgtcg ctatcggcgg cattacattg aaaaacgccg
     3421 gagatgtttt cggcgcgggc gcggattccg tcgccgttat cggcgacctg ctcggcgagc
     3481 cggacagaat catcgaaaaa atgaaacgct ttcttctgct gtaaaaaatt gtttatcaaa
     3541 gttaaaaata acttgctttt cgcccgacta ataagtaaaa atagaaatag cccgacggtt
     3601 a
//
LOCUS       DASWNE010000123         2438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_604579, whole genome shotgun sequence.
ACCESSION   DASWNE010000123 DASWNE010000000
VERSION     DASWNE010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604579"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..1917
                     /locus_tag="VGB68_02540"
     CDS             109..1917
                     /locus_tag="VGB68_02540"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048135.1"
                     /translation="MLELTQNPFFIGLIAFVAAVILTFAVRLLARRYGFVAKPKADRW
                     HQRPTAMMGGVGIYLTTVLMYLVFVEPHTRESWIILGGSSFLFLIGLIDDLLHVKPYQ
                     KLFGQLIGTAIVIGNGLVLAWTDSEIINIALTAFWLIGITNAINLLDNMDGLAAGIAA
                     IAALTLAVGLGATGQPYEFLLLSAFAGALIGFLIFNFNPASIFMGDCGSMFIGFFLAS
                     SVLLNQVGGRSRNIVSVLAVPALILLVPIFDTTFVTVLRKIWGKKASQGGRDHTSHRL
                     VALGLSERAAVLMLYAFAILAGATALLVRQLQPTQSIALIIFFVLILTIVGVYLAKVK
                     VYEEQDEELALKNNAAFGFLLNLSHKRRIFEIFLDVFLISLSYYASYTLIFGSIEETD
                     NWNLFLKTLPLLIVLKLFSFLAVGVYRGIWRYTSVQDFFTFAKGVIFGTVLSLMAILI
                     LYRFQNFSRTVFILDALILFAALAGSRMAFRFFREILLSPMKTSGRRVLIYGAGDGGE
                     LVLRELYNNPDWNYAPVGFVDDDPLKKDKVIRGLKVYGGNGSLAKICYDNAIEEILIT
                     SVKITPERLREIREICAETEVSLKRAQLKIEPVDFE"
     gene            2034..>2438
                     /locus_tag="VGB68_02545"
     CDS             2034..>2438
                     /locus_tag="VGB68_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0048136.1"
                     /translation="MTVKSVQLNSFVRHVILPAAALFCLLLVFFALKWYLANTISARA
                     EQKELAQLAIDLAPQDPQSYYALAVLNEKSFLPEDFSKSLAEFEQAAALSPNDYRLWF
                     ALGRARDRAGDSDGAELAMRKASELAPHYAQVQ"
BASE COUNT          497 a          616 c          645 g          680 t
ORIGIN      
        1 aggtttatta tgatttaatg cgggaaaaag gttagaatac cttttgcaga attttagaaa
       61 tacaaaccgg ttattcttcc cccatacgac cgagtcataa ttgttattat gcttgaactc
      121 actcaaaatc cctttttcat agggctaatt gcgtttgtcg cggcggtgat tttaaccttt
      181 gcggtgcgcc tgctggcgcg tcgttacggt ttcgtcgcca aaccgaaagc cgaccgctgg
      241 catcagcgcc cgacggcgat gatgggcggc gtgggaattt atctgacgac cgttttgatg
      301 tatctcgtgt ttgtcgagcc gcacacgcga gaatcgtgga ttatcctcgg cggcagctcg
      361 tttttgtttc tcatcggttt gatagacgac cttttgcacg tcaagccgta tcagaaactt
      421 ttcgggcagc ttatcggcac ggcaatcgtc atcggcaacg ggctggtgct ggcttggacg
      481 gattcggaaa ttatcaatat cgcgctgacc gctttctggc ttatcggcat caccaacgcg
      541 ataaatctgc tcgacaatat ggacgggctt gccgccggca tcgcggcaat cgccgctttg
      601 acgctcgccg tcgggctcgg cgcgaccgga cagccctacg agtttttgct gctttcggcg
      661 tttgcgggcg ctctgatagg ttttttaatt tttaatttca atccggcgtc gatttttatg
      721 ggcgactgcg ggtcgatgtt tatcggattt tttctcgcca gctcggttct tctcaatcag
      781 gtcggcgggc gctcgcgaaa catcgtatcg gttctggcgg ttccggcgct gattctgctg
      841 gtcccgattt tcgatacgac atttgtcacg gttctcagaa aaatctgggg caaaaaggct
      901 tcgcagggcg ggcgcgacca cacttcgcac cggctcgtcg cgctcggact ttcggaacgc
      961 gccgccgttt tgatgcttta cgctttcgcg attctggcgg gcgcgaccgc cctgctcgtg
     1021 cgccaattgc agccgacgca gagcatcgcc ctgattattt ttttcgtgct gattctgacg
     1081 atagtcggcg tctatctcgc caaagtcaaa gtttacgagg aacaggacga agagctcgcg
     1141 ctgaaaaaca acgccgcttt cggatttctg ctgaacctgt cgcacaagcg gcgcattttt
     1201 gaaatatttc tggacgtttt tctcatttcc ctttcttatt acgcgtctta cactttgatt
     1261 ttcggttcga ttgaagagac cgacaactgg aatcttttcc tgaaaacgct gccgcttttg
     1321 attgtgctca agcttttttc gtttctcgcc gtcggcgttt atcgcggcat ctggcgctac
     1381 acgagcgtgc aggatttttt cacttttgcg aaaggcgtca tcttcgggac ggttttaagc
     1441 ctgatggcga ttttgattct ctatcgtttt cagaattttt cgcggacggt ttttattctc
     1501 gacgcgctca ttttatttgc cgcgctggct ggcagccgga tggcgtttcg atttttccgg
     1561 gaaattttac tctcgccgat gaaaaccagc gggcggcgcg ttttgattta cggcgctggc
     1621 gacggcggcg agctggtttt gcgcgagctt tacaacaatc ccgactggaa ttacgctccg
     1681 gtcggatttg tggacgacga cccgctcaaa aaagataaag tcattcgcgg gctgaaagtt
     1741 tacggcggca acggctcgct ggcaaaaatc tgttatgata acgccatcga ggagattttg
     1801 attacttcgg taaaaattac gcccgaacga ttgcgcgaga ttcgtgagat ttgcgccgag
     1861 accgaagttt cgctgaaacg cgcgcagctt aaaatcgagc cggtagattt cgagtagcga
     1921 aacggaaagg aagatagaaa atcagggaaa ttaaggaagg aagaaccagg caaagagaat
     1981 atttttctcg tttcctgatt tcctgtcttc cctaatttcc tcttaaattt ttgatgaccg
     2041 ttaaatccgt tcaactaaac tctttcgtcc gtcacgtcat tctgccggcg gcggctttgt
     2101 tctgcctttt gctcgttttc ttcgcgctca agtggtatct ggctaatacg atttcagccc
     2161 gcgccgaaca aaaagagctc gcgcaattag ccatcgattt ggcgccgcaa gacccgcaat
     2221 cttattacgc gctggcggtg ctgaacgaaa aatctttttt gcctgaagat ttttcgaaat
     2281 cgctcgcgga atttgaacag gcggcggcgc tgtcgccaaa cgattatcgt ctctggttcg
     2341 cgctcgggcg cgcacgcgac cgcgccggcg atagcgacgg cgcggagctg gcgatgcgca
     2401 aagcatcgga gctggcgccg cattacgcgc aggtgcaa
//
LOCUS       DASWNE010000124        11592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_391055, whole genome shotgun sequence.
ACCESSION   DASWNE010000124 DASWNE010000000
VERSION     DASWNE010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11592
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391055"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VGB68_02550"
     CDS             <1..452
                     /locus_tag="VGB68_02550"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0048137.1"
                     /translation="RVEQFGANGDKPAPGDYDGDGKTDLAVFRPSDGSWYLLRSQQGF
                     TGVQFGIATDKPVQADYDGDGKTDLGVYRDGVWYLLQSQQGFAGIQFGIAADKPAPGD
                     YDGDGRADLAVYRQGIWYLLQSQAGFTGVQFGAANDRTIPSAYIPQN"
     gene            808..3960
                     /locus_tag="VGB68_02555"
     CDS             808..3960
                     /locus_tag="VGB68_02555"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY0048138.1"
                     /translation="MTKNLFSSSIRFFMLALFCLALGIGQSFAQSTVTGAIRGTVTDA
                     QGAVVPNATVTITNTGTNQTQDITTDEDGGYRFSNLQPGVYSLKTTASGFAQFTQERV
                     VVEVGQVTPIEVQLGLTGQTANVEVTAEAPVINTNSQDFATNINQTSINELPINGRRA
                     SNFVLLTPATVPDGTFGLISFRGISGLLNNSTVDGGDNNQAFQSEERGRTRIGYVISQ
                     SAIREFQVNTSNYSAEYGRSAGGVVNTVTKSGTNEFHGDLFEYYRNNRFGARNPLAFR
                     TILNNGVQTVVGLKPVDIRHQFGGTIGGPIVKDKLFFFFSYDQQKRNFPGLAIFSNLN
                     YLNTVNRPLLQSRGLTTAQIDNTLNFINSLTGETPRRQDQKLFLPKIDWQINNNHLFS
                     VSYNRLRADSPAGLQTQATNTFGRRSFGDDFVDVDSLNARLQSTITPNILNEARFQYS
                     RDNEYAFSQEPLPGEPAIVQTAAGLRSPSINLTGGLTFGTTANFERVKFPDEKRIQIA
                     DSVTWTRGRHTIKFGGDFNHIVDDIENLRSQTGSYIYSTINDFIIDYVNYVNPNTLAA
                     TTACATGTNRVGRCYNGTFLQGVGIPGLKLSTNEYNAFIQDDFRITPRLTVNLGLRYE
                     YQQLPTPILANTSTAIIPNDLRTVNEATSTLPDDRNNFGPRFGFAYDLTGDGKTSIRG
                     GVGIYYGRIATAQIYNALLNTGNPGGAGQITVANTAANAPIFPNILPTNSPLNLAAIQ
                     FYQANFQAPQITQYDLILERQVMRNTVVSIAYLGSRGAHLPTFVDQNLRPTGATTTFN
                     VVGGPAAGQTFTLPQYTRANTAFQQLTQIQSSVKSEYNALVLQANRRFTNGLQFQTSY
                     TLARATDTNQNSSVFPVNNSPFDPFDRSYDAGYSNNDVRHKFVISAVFAPTFYKGDAG
                     SVGNYLLNGWSIAPIYTFYSGRPFSGTTSGATLNGTNGDTMFPLLPRNSFRLPSVSNL
                     DLRLSKRFRFTERYNLELLAEGFNVFNKTFTFIQTNLLYQRATGSNTLTYNPNFGGTT
                     ESSSSNYRERQIQFAARFQF"
     gene            4063..5160
                     /locus_tag="VGB68_02560"
     CDS             4063..5160
                     /locus_tag="VGB68_02560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0048139.1"
                     /translation="MRILQISSARNFGGGEKHLLDLCRGLNGRGHEVFIALRPTNKWQ
                     EKFDFLPPENFLHVSIRNSFGIFSAQRIADFAREKNIEIIHAHVARDYIPASLACRIA
                     KTTKLVLTRHVLFPMKPFHKFALRNVSRVIAVSGAVESNLEQIFPREKITGVLNGIEI
                     ETWSNANRENLRRDFRSGHNISSDSLLIGAVGELKLLKGQRDFVLAAKIIAEKFPEAH
                     FVVVGKDNSVNQSFRNELKRLVRVFNLENRFLWLDWVEETAPLLHALDIFVSASHSES
                     FGLAILEAMASGTPIAATATEGAKEILRHDETGLLVPVQEPVALAEAIGRLLADENLR
                     VRLGKQAREFAAGNFSLERMIEETEKVYKQI"
     gene            complement(5188..5859)
                     /locus_tag="VGB68_02565"
     CDS             complement(5188..5859)
                     /locus_tag="VGB68_02565"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEY0048140.1"
                     /translation="MKRAIIIMAKAPLAGTVKTRLQPFLSAEQCARLAACFLRDTINK
                     AKTLKIQLIIAYSPAGEIDYFRRSADEKTSFVEQKGDNLGEKMFSAFRFAFARKTEAL
                     VMIGTDSPTFPADFIKQAFELLETDSDAVLGKTEDGGFYLLGLRKLDEKIFEAVEWSS
                     PKAFEQVRQNIMNLSWRLSEVPRWYDIDEAKDLEQLKNEFLNNKNAPRRAPQTFEWIT
                     KNINK"
     gene            5904..6395
                     /locus_tag="VGB68_02570"
     CDS             5904..6395
                     /locus_tag="VGB68_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048141.1"
                     /translation="MPENFRTGAVGAMMDEYERAAREFQSLVRETGEDEFTRIADSET
                     GDESCRSIQTITTHVVGAGCGYANYIRKNFSMSSEPPSREPISHGEASGKIDKMLAYT
                     IETLDGKWEMSDDEIDKIVIHSGWGVTYNLEQLLEHAIVHVLRHRRQVEKFLAKFEIE
                     KSS"
     gene            6428..6757
                     /locus_tag="VGB68_02575"
     CDS             6428..6757
                     /locus_tag="VGB68_02575"
                     /inference="COORDINATES: protein motif:HMM:TIGR04169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis-associated
                     peroxidase-like protein"
                     /protein_id="HEY0048142.1"
                     /translation="MHYYNEEDLARFGEIGKTKPELFEKFMAWYQACQEDGALSKREK
                     ALIGLAVAVTVQCPYCIDAYTQSSLENGSNMEQMTEAIHLASAIRGGASLIHGIQSRN
                     AHDKISM"
     gene            6772..7155
                     /locus_tag="VGB68_02580"
     CDS             6772..7155
                     /locus_tag="VGB68_02580"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0048143.1"
                     /translation="MSDEARKKLVKTYVQAYNRFDVEAMLSVLHDEIVFENISDGELT
                     LEIKGIEAFRNQAEQAAKFFAERTQEIEDFVFSDQGCEVNIDYRGIIAADLPNGLKTG
                     DEIKLKGKSIFRFADDKISKIQDIS"
     gene            7159..8163
                     /gene="arsS"
                     /locus_tag="VGB68_02585"
     CDS             7159..8163
                     /gene="arsS"
                     /locus_tag="VGB68_02585"
                     /inference="COORDINATES: protein motif:HMM:TIGR04167.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HEY0048144.1"
                     /translation="MQAVQITRRGKPALPQSSVGFDDKLGAHGLKLRSTGVEILQVNV
                     GKLCNQACKHCHVDAGPKRSEIMAAETVEACLNLLRSFKIPTLDVTGGAPELNPSFRY
                     LVAEARKTGARVMVRHNLTVMFEPQQTDLPGFFSENEVEVVSSLPYFLQQQTDAQRGT
                     GVFEKSIEAIKRLNDFGYGIEGSGLLLNLVYNPTGAFLPPAQEAIEADFKRELKNRYN
                     LHFNNLYTITNMPIARFLDWLRRSKNEESYMRKLINAFNPATVEGLMCRNTLSVDWRG
                     FLYDCDFNQMLEIGVDERLPQTIFDFTPETFTSREIRTAVHCFGCTAGAGSSCGGTVA
                     "
     gene            complement(8244..10040)
                     /locus_tag="VGB68_02590"
     CDS             complement(8244..10040)
                     /locus_tag="VGB68_02590"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY0048145.1"
                     /translation="MWKKFLAFQLLILLAMPFAVFAQTKQKISLLLVNGKIFTADEQF
                     SVAEAVAVDGEKIVAVGKTSELKARYQAAREIDLSGKLVTPGFNDAHVHFLRGALALL
                     NVNLLDAKSLEEAKARIAAKAKEVKPGEWIFGRGWDHTIWKQNFPSRKDLDQVAPNNP
                     VYLVRVDGHVAWANSAALRLAGIDRNTPNPEGGEIEKDAAGEPTGILKETAQGLVNRL
                     IPQPDQPTLFKGLEMALDMARRYGITSVQDNSGYETTKLYRAFLQQDKLTVRVSEWQD
                     FEDSIEKLKAQRAEFASFKDNPSRLKLGALKGYVDGTLGSKTAAMLAPFSDDEGNSGI
                     PRRSAEDLTRMIVERDKEGFQIALHAIGDRANRMALDGFRAARSTGTAVILPSRDNQK
                     LKIAPVLSSRTNPCLNQEVAFKSGAVKMSNILECRHRIEHAQVVSPSDFKRFAELGII
                     ASMQPSHAISDKRWAESRLGEYRVLGAYAWHFMQSYGVHVPFGTDFPVEPINPYNTLY
                     SAVSRQDAEGDPLGGWQPQERLTMAEAIRCHTYESAYAEFSEKEKGEIKEGMLADLVV
                     HSKDLLAIEPKEILTAEAVLTVFNGRVIYEKK"
     gene            10166..10852
                     /locus_tag="VGB68_02595"
     CDS             10166..10852
                     /locus_tag="VGB68_02595"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY0048146.1"
                     /translation="MKNETLQFNTANGASTAYVATPDEANGKAVILIHEWWGLNDHIK
                     DITNRYAEEGFTAIAPDLYRGKVTKDKEEAGRLMQNLELEDGLNTIQMAIGKAQETYG
                     IEKFGITGFCMGGTFALRAACELEHIGAAAPFYGDIPDEFTLKDLKCPVIFVSGTKDQ
                     WINPEKVGELERIAADNFLPVKSLKYDADHAFFNDTRPEVYDETAAKDAWSKVIAFFN
                     ENLEIVTVSL"
     gene            complement(11020..>11592)
                     /locus_tag="VGB68_02600"
     CDS             complement(11020..>11592)
                     /locus_tag="VGB68_02600"
                     /inference="COORDINATES: protein motif:HMM:NF014298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEY0048147.1"
                     /translation="RKGNFAHYGVVENAHARHILDVSFAPALISEKTYGEAVKIARAV
                     AVALDYVGTLCVEFFLAPGEKLLINELAPRPHNSGHLTFDACITSQFEQQLRAVCGLP
                     LGSTAFYKPAAMANLLGDIWRTGEPNWARSLEFPNLKLHLYGKAEARAGRKMGHLTAL
                     AATAREAVETVRKAREELAAKKDEKARNTN"
BASE COUNT         2919 a         3011 c         2879 g         2783 t
ORIGIN      
        1 tgcgcgtcga acaattcggt gcaaacggcg ataaacccgc gccgggtgat tatgatggcg
       61 acggcaaaac ggatttggcg gtttttcgcc cgtccgatgg aagctggtat ttgctgagaa
      121 gccagcaggg atttaccgga gtccaattcg gaatcgcaac cgacaagccg gttcaggcgg
      181 attatgacgg cgacggaaag accgatttag gcgtctatcg cgatggcgtt tggtatttat
      241 tgcagagcca gcagggtttt gcgggaattc aattcggcat tgcggctgac aaacccgcgc
      301 ccggcgatta cgacggcgac gggcgggctg atttagccgt ttaccgccag gggatttggt
      361 atctgctgca aagccaggct ggatttaccg gcgtgcaatt cggcgcggca aacgacagaa
      421 cgattccgtc ggcttacatt ccgcaaaatt aatttcgcgc cgcgctgtaa caccgattta
      481 attttcgaac tgcataatta catttatgct tttcgacaaa aatttaagga actcaatttg
      541 cctaaaagtt tattgactat cttatgcaaa cccacaattt gtcgaaaaat attttcataa
      601 cgagcgttat gaaagcgcta atcgtgtgag tttacaataa gttggaaagt ttactgaaat
      661 ccgtttagga attttaacct tgaattaatc gaaaattctt cgttataaaa tccaaaaaat
      721 aaaattcaaa atccgaaaat caaaatgttt tcgaatttct acacgcaatt ttttaaaatt
      781 caaattcaag atatgaggtc agaaagaatg actaaaaatt tattttccag ttcgatacgc
      841 ttttttatgc tggctctctt ttgtctcgcc ttaggcatcg ggcagagctt tgcacaatca
      901 accgtaacgg gcgctattcg cggcacggtg accgacgcgc agggcgcggt cgtacctaat
      961 gccaccgtta ctattaccaa caccggaacg aatcaaacac aagatataac tactgacgag
     1021 gacggcggtt accgcttcag caatcttcaa cccggagttt attcgctcaa aacgacggct
     1081 tcgggctttg ctcaatttac gcaggaaaga gtcgtcgtcg aagtcggtca ggtcacgccg
     1141 attgaagttc agctcggctt gaccggacaa accgccaacg tcgaagtgac ggcggaagca
     1201 ccggttatta ataccaactc gcaggatttt gcgaccaata tcaaccaaac ctcgattaac
     1261 gaactgccga ttaacggtcg tcgcgcctca aactttgtgc tgctgacgcc cgccaccgtc
     1321 ccggacggaa ccttcggctt gattagtttt cgcggaattt ccggactgct gaacaacagc
     1381 accgtcgacg gcggcgacaa caaccaggcg tttcaatccg aagaacgcgg tcgcacgcgc
     1441 atcggctatg ttatcagcca atcggcgatt cgcgagtttc aggtcaacac ttcaaattac
     1501 tcggctgaat acgggcgctc ggctggcggc gtggtcaaca ccgtaaccaa aagcggaacc
     1561 aacgaatttc acggcgacct ttttgagtat taccgcaata atcgcttcgg cgcgcgcaat
     1621 ccgctggctt tccgaacaat tttaaataac ggcgttcaaa cggttgtcgg tttaaaaccg
     1681 gtcgacattc gccatcaatt cggcggaaca atcggcggtc cgattgttaa agataaattg
     1741 tttttctttt tcagctacga ccagcagaaa cgaaactttc cgggtctggc tatattcagc
     1801 aacttaaact acctgaatac ggttaaccgc ccgcttctgc agagcagagg attaacgacc
     1861 gcccaaatcg acaacacgct gaattttata aactctttga ccggcgaaac tccgcgccgc
     1921 caggaccaga agttattttt gccgaaaatt gactggcaaa tcaataacaa tcatctgttt
     1981 tcggtaagct acaatcgcct gcgcgccgac tcgcccgccg gtttgcagac acaagctacc
     2041 aataccttcg gacgcagaag tttcggcgac gactttgtag atgtcgattc tttaaacgcg
     2101 cgtctgcaat cgacgattac tcctaatata ttgaacgaag cgcgtttcca gtattcgcgc
     2161 gataacgaat acgccttcag tcaggaaccg cttcccggcg agccggctat tgtccagacg
     2221 gctgccggac tgcgctcgcc gagtataaat ttaaccggcg ggttgacctt cggcacgacc
     2281 gcaaactttg agcgcgtcaa gtttcccgac gaaaaacgca tccagattgc cgactcggta
     2341 acctggacca gaggcagaca cacgataaag ttcggcggcg attttaatca tatcgtcgac
     2401 gatattgaaa atctgcgttc gcaaaccggc tcttacattt attccacaat caacgacttt
     2461 attattgatt atgtaaacta tgttaatccg aatacgctgg cggcgacgac ggcgtgcgcg
     2521 acgggaacca accgggtcgg aagatgctat aacggcactt ttctgcaagg cgtcggcatc
     2581 ccgggcttaa agctcagcac gaacgaatac aatgcgttca ttcaggatga ttttcgaatc
     2641 acgccgcgct taaccgttaa tttgggactg cgttatgaat atcagcagct tccgacgccg
     2701 atattggcaa acaccagcac ggcgattatc ccgaacgacc tgagaaccgt aaacgaagcg
     2761 acttcgactc tgcccgacga cagaaacaac ttcggtccgc gtttcggttt cgcttacgac
     2821 ttaaccggag acggcaaaac gtcaatccgc ggcggcgtcg gcatctacta cggtcgcatc
     2881 gccaccgcgc agatttacaa cgcgctgctg aacaccggca atccgggcgg cgcgggacag
     2941 ataaccgtcg ccaatacggc ggcaaacgct ccgattttcc ccaatatttt accgaccaac
     3001 agcccgctta atctggcggc gattcagttt taccaggcta attttcaagc tccgcaaatt
     3061 acccagtatg acttgattct ggaacgtcag gtgatgcgaa acacggtcgt ttcaatcgct
     3121 tatctgggaa gcagaggcgc gcatctgccg acgtttgtcg accagaacct gcgaccgacc
     3181 ggagccacga caacatttaa cgtggtcggc ggaccggctg ccggtcagac ctttacgttg
     3241 ccgcaataca cccgcgccaa cacggcgttt caacagctga ctcaaattca aagctcggtt
     3301 aaatccgagt ataatgcttt ggttctgcaa gccaaccgtc gctttaccaa cggtttgcag
     3361 ttccagacca gctacactct ggcgagagcg accgatacga accagaactc gtcggtcttc
     3421 cccgtcaaca actcgccgtt cgaccctttc gaccgcagct atgacgccgg ctacagcaac
     3481 aacgacgttc gccataaatt tgttatcagc gccgtttttg cgccgacttt ttataaaggc
     3541 gacgccggtt cggtcggcaa ctacctgttg aacggctggt cgattgctcc gatttacact
     3601 ttctattcgg gtcgcccgtt tagcggcacc acttccggcg cgaccttgaa cggcactaac
     3661 ggagatacta tgtttccgct tctgccgcgc aactctttcc ggctgccgag tgtttccaac
     3721 cttgatttga gactttcgaa acgtttcaga ttcacggaaa gatacaatct cgaattgctg
     3781 gctgaaggat tcaacgtctt taacaagact ttcacgttta ttcaaacgaa cctgctttat
     3841 cagcgcgcga ccggcagcaa tacgctgacc tacaatccga acttcggcgg cacgaccgaa
     3901 tcgagcagct caaattatcg cgagcgtcag attcagtttg cggcgcgttt ccaattttag
     3961 tttttcctca ccgggaaaaa cctttcggcg tgtttccttt ttgggagcac gccattttta
     4021 ttttcgattg ccgatttgcg atttccgttc tacgatttac gaatgagaat tctgcaaatc
     4081 tcatcggcaa gaaatttcgg cggcggcgaa aagcatctgc tcgatttgtg tcgcggcttg
     4141 aacgggcgcg ggcacgaagt ttttattgcc ttgcgcccga ccaataagtg gcaggaaaaa
     4201 ttcgattttc tgccgccgga gaattttctg cacgtttcca tacgcaattc cttcggaatc
     4261 ttcagcgcgc agcgaattgc cgatttcgcg cgcgaaaaaa acatcgaaat cattcacgcc
     4321 cacgtcgcgc gcgattacat tccggcgagt ctcgcctgcc gcattgcgaa aacgacaaag
     4381 ctggttttaa cgcgccacgt cctttttccg atgaagcctt ttcataaatt tgctttgaga
     4441 aacgtctcgc gcgtcatcgc cgtctcgggc gcggtcgaaa gcaatctcga acaaattttt
     4501 ccgagagaaa aaattaccgg cgttttgaac ggaatcgaaa tcgaaacgtg gtcaaacgcg
     4561 aatcgtgaaa atttgcggcg ggactttcgc tccggacata atatttcttc cgactcgctt
     4621 ttaatcggcg cggtcggcga gctgaagctt ttaaaaggtc agcgcgattt cgttctggcg
     4681 gcgaaaatca tcgccgaaaa atttcccgaa gcgcattttg tcgtcgtcgg caaggataat
     4741 tccgttaatc agagcttcag aaacgagctg aaacggctgg tcagagtttt caatctcgaa
     4801 aatcgctttt tgtggctcga ttgggtcgaa gaaaccgcgc cgctgcttca cgcgctggac
     4861 attttcgttt cggcttcgca ctcggaaagc ttcgggctgg ctattttgga agcgatggca
     4921 agcggaacgc cgattgcggc aacggcgacc gagggcgcga aagaaatttt gcgccacgac
     4981 gagacgggtt tgctcgtccc cgttcaggag cccgtcgctc tagccgaagc aatcggcagg
     5041 cttttagccg acgaaaatct gcgcgttcgg ctcggcaaac aggcgcggga atttgccgcc
     5101 ggaaattttt cgctcgaacg aatgattgag gaaaccgaaa aagtttacaa acaaatatag
     5161 cggcgtgcgg ctgcatttcc tgcggcgtta tttatttata tttttcgtaa tccattcaaa
     5221 agtctgcggc gcgcggcgcg gcgcgttttt gttattcaga aattcgtttt tcaattgctc
     5281 caaatccttt gcttcgtcaa tatcatacca gcgcggaact tcgctcagtc gccagctcag
     5341 attcataata ttttggcgca cctgctcaaa agctttcggc gagctccatt cgaccgcttc
     5401 aaaaattttc tcgtccaatt ttcgtaatcc gagcaggtaa aatccgccgt cctcggtttt
     5461 tcccagaacg gcgtcggaat cggtttccaa aagctcgaac gcctgcttga taaaatcggc
     5521 gggaaacgtc gggctgtccg tgccgatcat caccagcgcc tccgttttac gggcgaaagc
     5581 gaaccggaaa gcggaaaaca ttttttcgcc cagattgtcg cctttttgtt cgacgaaaga
     5641 cgttttttcg tctgccgatc gccgaaaata atctatttct ccggcgggcg aatatgctat
     5701 tatcaattga atttttaatg ttttcgcttt gtttatcgta tcgcgcaaaa aacacgcggc
     5761 aagtcgcgcg cattgttcgg cggaaagaaa gggctgaagc cgcgttttga cggttccggc
     5821 aagcggcgct ttcgccataa tgataattgc gcgtttcatt ttcgaagaaa ttttataact
     5881 tttccggaat ttaagaaaaa ttgatgcccg aaaattttcg cacaggcgcg gtcggcgcga
     5941 tgatggacga atacgagcgc gcggcgcgcg aatttcaatc tctggttcgg gaaaccggcg
     6001 aagacgagtt tacgcggatt gccgacagcg aaaccggaga cgaatcctgc cgctcgattc
     6061 aaacaatcac gacgcacgtc gtcggcgcgg gctgcggtta cgcaaattat attcgcaaaa
     6121 atttttcgat gtcttccgag ccgccttcgc gcgagccgat ttctcacggc gaagcgagcg
     6181 gcaaaattga taaaatgctg gcttatacaa tcgagacctt agacggaaaa tgggaaatgt
     6241 ccgacgacga aatcgacaaa atcgttattc attccggctg gggcgtgacc tacaacctcg
     6301 aacaattgct cgaacacgcg attgttcacg ttctccggca cagaagacag gtagaaaaat
     6361 ttctggcgaa atttgaaatt gaaaaatcgt cttgaaatct taatcgcaaa gtttaaaact
     6421 aataaatatg cactattaca atgaagaaga tttagcgcgc tttggcgaaa tcgggaaaac
     6481 gaagcccgag cttttcgaaa aatttatggc ttggtatcag gcttgccagg aagacggcgc
     6541 gctttcaaaa cgcgaaaagg ctctgattgg actggcggtc gccgtgactg tgcaatgccc
     6601 gtactgcatc gatgcctaca cgcagtcgtc gctggaaaac ggctcgaata tggagcagat
     6661 gaccgaagcg attcatctgg cttcggctat acgcggcggc gcaagcctga ttcacggcat
     6721 ccaatcccgc aacgcgcacg ataaaatttc gatgtaattt cggcaaaatt tatgtctgac
     6781 gaagcaagga agaaactggt gaaaacttac gttcaagctt acaaccgctt cgatgtcgaa
     6841 gcaatgttgt cggttctgca cgatgaaata gtttttgaaa acatctcgga cggcgaactg
     6901 acgcttgaga ttaaaggaat cgaggctttt agaaatcagg cggaacaggc agcaaaattt
     6961 ttcgccgaaa gaacgcagga aatcgaggat tttgttttca gcgaccaggg ttgcgaggtc
     7021 aatatcgatt atcggggtat aattgccgcc gatttgccga atggattaaa gacaggcgac
     7081 gaaatcaaac taaaaggcaa atcaattttt cgcttcgccg acgacaaaat ctctaagatt
     7141 caagacatta gctaaaatat gcaagctgtc caaatcactc gtcggggcaa gcccgcgctg
     7201 ccgcaatcgt ccgtcggttt tgacgataaa ctcggcgcgc acggcttaaa actgcgctcg
     7261 accggcgtcg aaattcttca ggtcaacgtc ggaaagctct gcaatcaagc ctgtaaacat
     7321 tgtcacgtcg acgccggtcc gaagcgctcg gaaattatgg cggcggaaac cgtcgaagcg
     7381 tgtctgaacc tcttgcgcag ctttaaaatt ccgacgctcg acgtgacggg cggcgcgccg
     7441 gagttgaatc cttcgtttcg ctatctggtc gccgaagcgc gaaaaaccgg cgcgcgcgtg
     7501 atggtcaggc acaatctgac cgtgatgttc gagccgcaac agacggattt gcccggattt
     7561 ttttcggaaa atgaagtcga agtagtctcc agcctgccct attttttgca gcagcagacg
     7621 gacgcgcagc ggggcacggg cgtttttgaa aaaagcatcg aagcgataaa acgtctgaac
     7681 gatttcggct acggcatcga aggcagcggc ttgctgctga atctggttta caatccgacg
     7741 ggagcatttt tgccgccggc gcaggaagcc atcgaagccg atttcaaacg cgagctgaaa
     7801 aatcgataca atctgcattt caacaatctc tacacgatta ccaatatgcc gattgcgcgc
     7861 tttctggatt ggctcaggcg ctcgaaaaac gaagaaagct atatgaggaa gctgatcaac
     7921 gctttcaatc cggcgaccgt cgagggctta atgtgccgca acacgctcag cgtcgattgg
     7981 cgcggttttc tgtacgactg cgattttaat caaatgctgg aaatcggcgt cgacgagcgc
     8041 ctgccgcaga cgattttcga ttttacgccg gaaacattta ccagccgcga aatcagaacg
     8101 gcggtgcatt gcttcggctg tacggcgggc gccggctcaa gctgcggcgg cacggtagct
     8161 taaaaagcgg aaagcggaga aacggaaaat ttaaaaacac atattttccg tttttccgct
     8221 ttccgctctt ttgcggctga ttcttatttc ttctcgtaaa tcactcgccc gttaaaaacg
     8281 gtcaggacgg cttcggcagt taaaatctcc ttcggctcaa tcgccagtaa atctttggag
     8341 tgaacgacca aatcagccag cattccctct ttgatttcgc ctttttcctt ttcggaaaat
     8401 tcggcgtaag ccgattcgta agtgtggcag cgaatcgctt ccgccattgt taatctctct
     8461 tgcggctgcc agccgccgag cgggtcgccc tcggcatcct gccgcgaaac ggcggagtag
     8521 agcgtgttat acggatttat cggctcgacc ggaaaatccg tcccgaacgg aacgtgaacg
     8581 ccgtaagact gcataaaatg ccacgcatac gcgcccagaa cgcgatattc gcccaaacgc
     8641 gattccgccc agcgcttgtc ggaaatcgcg tgcgaaggct gcatcgaagc gataataccg
     8701 agctcggcaa aacgcttgaa atcggagggc gaaacgactt gcgcgtgttc gatgcgatgg
     8761 cgacattcca gtatattaga catttttacg gcgcctgatt taaaggcaac ttcctgattc
     8821 aaacaaggat ttgttctgga gctcaaaacc ggcgcgattt tcagtttctg attatcccgg
     8881 cttggtaaaa taacggctgt tccggtcgaa cgagcagcgc gaaatccatc caaagccatt
     8941 cggtttgccc ggtcgccaat cgcgtgaagc gcaatttgaa aaccttcctt atcgcgctcg
     9001 acaatcattc gcgtcaaatc ttccgccgag cgccggggaa tgccggaatt accttcgtcg
     9061 tcggaaaaag gcgcgagcat cgcggcggtt ttcgagccta aagttccgtc gacgtagcct
     9121 ttcaaagcgc ccaatttcaa acgcgacgga ttgtctttga aagaagcgaa ctcggcgcgc
     9181 tgggctttca atttttcaat cgagtcttca aaatcctgcc attccgaaac gcgcacggtc
     9241 agtttgtcct gttgcaaaaa cgcgcggtaa agctttgtcg tctcgtaacc ggaattgtcc
     9301 tgcaccgaag taatgccgta acgccgcgcc atatcgagcg ccatctccag acctttgaaa
     9361 agcgtcggct ggtcgggctg cggaatcagg cgattgacca agccctgcgc ggtttccttc
     9421 aaaattccgg tcggctctcc ggcagcgtct ttttcaatct cgccgccttc cggattcggc
     9481 gtgttccggt ctattcccgc cagccgcaaa gccgccgaat tcgcccacgc gacgtgaccg
     9541 tcaacgcgaa cgagataaac cggattatta ggcgcgacct ggtcgaggtc ttttcgggac
     9601 ggaaaatttt gtttccagat ggtgtggtcc cagccgcgcc cgaaaatcca ttcgccgggc
     9661 tttacttcct tcgctttcgc cgcgatccgc gctttcgctt cttcgagcga tttcgcatcg
     9721 agcagattca cgttcaaaag cgccagcgcg ccgcgcagaa aatgaacgtg cgcgtcattg
     9781 aatccgggcg tcactagttt tccgctcaaa tcaatttccc gcgccgcctg gtatctggct
     9841 tttaactcgc tcgttttccc gaccgcgacg attttttcgc cgtcgacggc aaccgcttcg
     9901 gcgacggaaa actgctcgtc agccgtgaaa attttgccgt tcacaagcag cagagaaatt
     9961 ttctgttttg tttgcgcgaa aaccgcaaaa ggcatcgcca gtagtattaa taattgaaaa
    10021 gctaaaaatt ttttccacat agattttaaa gtccggcaga atttttataa cacttaatcg
    10081 agcatcggaa aagttttcgc agacgaaatt tcgcgtcttc gctcgggcat gtttataatt
    10141 agaacaaaat tttcaaggag gaattatgaa aaacgaaact ttacaattta ataccgccaa
    10201 cggcgcttcg accgcttacg tcgccacgcc cgacgaggcg aacgggaaag ccgtgatttt
    10261 aattcacgaa tggtggggct taaacgacca catcaaggac attactaacc gctacgccga
    10321 agaaggcttt acggcaatcg cgcccgacct gtatcgcgga aaggtcacaa aagacaagga
    10381 agaagccgga agattaatgc agaatctcga actcgaagac ggtttgaaca cgattcagat
    10441 ggcgatcggc aaggcgcagg agacctacgg catcgaaaaa ttcggcatta cgggcttttg
    10501 tatgggcgga actttcgcgc tgcgcgccgc ctgcgagctg gaacacatcg gcgcggcggc
    10561 tccgttttac ggcgacattc ccgacgaatt tactttaaaa gatttaaaat gtccggtcat
    10621 attcgtgtcg ggaaccaaag accaatggat taacccggaa aaagtcggcg agttggaaag
    10681 aatcgctgcc gataactttc tgccggttaa gtcgctgaaa tacgacgccg accacgcttt
    10741 ttttaacgac acgcgaccgg aagtttacga cgaaacggcg gcaaaagacg cctggtcgaa
    10801 agtcatcgca tttttcaatg aaaatttgga aatagtcacg gtaagtttgt aaaattaaaa
    10861 gaccgctcac gaaataaaag tcagccgccg ggagcacaaa gatttttaag aaaaattaac
    10921 cgccgacgag cgcgaataaa agaggatttt ttcctgctcg tctctgtccg taaggttaat
    10981 ttccgtctgt cttgtcagcg ctctcggcgg ctgactttct taattcgtat ttcgtgcttt
    11041 ttcgtctttt ttcgcggcta attcctctct cgcttttctc acggtttcga ccgcttcgcg
    11101 cgcggtcgcc gccagcgccg tcaggtgtcc cattttgcga ccggcgcgcg cttcggcttt
    11161 gccgtaaaga tgaagcttga gattcggaaa ctccaggctg cgcgcccagt tcggctcgcc
    11221 cgtccgccag atgtcgccga gcagattcgc catcgccgcc ggtttataaa atgcggtcga
    11281 acccaaaggc aagccgcaga cggcgcgtaa ttgctgttca aactgcgagg tgatgcaggc
    11341 gtcgaaagtc aggtgaccgg aattgtgcgg gcgcggcgca agctcgttaa tcagcagctt
    11401 ttcgcccggc gcgagaaaaa attcgacgca taaagtcccg acgtaatcga gagcgacggc
    11461 gacggcgcgc gcgattttga cggcttcgcc ataagttttt tcggaaatga gcgcgggcgc
    11521 gaaagaaacg tctaaaatat gtcgggcgtg cgcgttttcg acgacgccgt aatgagcgaa
    11581 attgcctttc cg
//
LOCUS       DASWNE010000125         5063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_463563, whole genome shotgun sequence.
ACCESSION   DASWNE010000125 DASWNE010000000
VERSION     DASWNE010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463563"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VGB68_02605"
     CDS             <1..226
                     /locus_tag="VGB68_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048148.1"
                     /translation="FTRKMFGASIKQVDFVLRQVVQLPEIIKPGGNRVKKTRGSEISR
                     RRAPMRILPPSPSLAINHSTKNQPAARICQ"
     gene            520..1128
                     /locus_tag="VGB68_02610"
     CDS             520..1128
                     /locus_tag="VGB68_02610"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY0048149.1"
                     /translation="MRIIDALVRWLAYGGVHPNVLTVIGVTINAACGVLFGFGEFFWA
                     GIVLIVANLFDMLDGNVARLSGNVTRFGAYLDSSLDRLSDMVCFLGIIVFYASNTPQH
                     SLLNVFLAGMGMVGSVMVSYTTARSESMGVKANVGFLQRPERIVLFIIGALSTHWGST
                     NFFANRMPQVLWVLAIGSFWTFIHRMYYTWIELNKTEIAQEN"
     gene            1135..1560
                     /locus_tag="VGB68_02615"
     CDS             1135..1560
                     /locus_tag="VGB68_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048150.1"
                     /translation="MDWLSGLWDSLTLDRILLFGGLFLASLAFSFIVLGVVMVKIPED
                     YFSSHYQQKFMPNSSFLVRWGAVILKNLLGVFLIILGIILSLPGVPGQGLLTILLGLI
                     MLDIPGKRPLEARIIKRPTILAAINNLRGRFNKPPLVLD"
     gene            1563..1850
                     /locus_tag="VGB68_02620"
     CDS             1563..1850
                     /locus_tag="VGB68_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048151.1"
                     /translation="MGLFSIFKQKKNVDLEAERRERLRQKGRICDGTIIDSETNAQGE
                     IVFYVYSVHGVDFESSELLSEEQLIDPLKYAPGAKVGVRFDPKNHGNSMLV"
     gene            1923..3437
                     /gene="accC"
                     /locus_tag="VGB68_02625"
     CDS             1923..3437
                     /gene="accC"
                     /locus_tag="VGB68_02625"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEY0048152.1"
                     /translation="MFQKILIANRGEIACRVIRACRDMKIKTVAVYSEADKNALHVRM
                     ADEAYFIGAPPSNESYLRWEKIIDVAKKSGAQAVHPGYGFLSENAEFVRQVNAEGIIF
                     IGPSPEAMEAMGGKISARKIAIEAGVPVVPGTVEPLQSAGEAAEVAKKFGFPVMLKAS
                     AGGGGKGMRFVENESDLKSALETAQSEAMAAFGDASVYVEKAIVRPRHIEIQIFSDTH
                     GNHVHLGERECSIQRRHQKVIEECPSPINDANLRQRMGECAVKVARAVDYVGAGTVEF
                     LVSDETREFYFLEMNTRLQVEHPVTELVTGIDLVREQIRVAWGEPLSFKQEDVTWNGH
                     AIECRVYAEDPENNFLPSPGRINRLRVPHGNGVRDDGGVYEGAEVSIYYDPMISKFAA
                     YGKNREEAIDRLRRALEEYEVGGIKTTLPFFREIVTDTEFIEGNLDTGFISRWQERRA
                     KREATEIEKDMALIAAALAYTDKQKAISKQADGNSTGASRWVMAGRTAMLTNRL"
     gene            3464..3673
                     /locus_tag="VGB68_02630"
     CDS             3464..3673
                     /locus_tag="VGB68_02630"
                     /inference="COORDINATES: protein motif:HMM:NF041655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MM0924 family protein"
                     /protein_id="HEY0048153.1"
                     /translation="MEEFLTQLMGKRIDISCGSTAVVRGDVMAVKDGVLYLRDEDERV
                     AYVAIEKVAIVWEVKEHQTRPGFVG"
     gene            complement(3896..4579)
                     /locus_tag="VGB68_02635"
     CDS             complement(3896..4579)
                     /locus_tag="VGB68_02635"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEY0048154.1"
                     /translation="MIWKKPSCVFLFFAALFMFSNAADAQVYSRPRIVPTIPDASTTT
                     PKPSQTTQSNVTQRPTLMNKIVVASQTPSQPQQQPLVRKTVDSRPVNPAVANLAGSLV
                     YSPSFSQKMQQSIQAKLGIRYVYGTEGPNTYDCSGFVWSVFRDAGFSFERTSARSLWA
                     GSEPVAEDERFKFGTLVFFNGLGHMGIVADANGFYHASSSKGITYSPFAGYWGKRIVG
                     FRRLPVKTN"
BASE COUNT         1276 a         1150 c         1369 g         1268 t
ORIGIN      
        1 attcacgaga aaaatgtttg gagcaagcat aaaacaagtc gattttgtgt tgcgacaagt
       61 cgttcaatta ccggaaatta taaagccggg cgggaaccgg gtgaagaaaa cccgcggcag
      121 cgaaatcagc cgacggcgtg cgccgatgcg aatattgccg cccagcccga gcctggcaat
      181 aaatcattca acaaaaaacc agccggcggc gaggatttgt caataacatt gcctcgccgc
      241 ccgctgcgcg gcaacggtat agcgcgatta atcgctgccc gtccgaaacg caattcttta
      301 tcgcccgaat aacattttat aacttcatcg acccaatcgc gtttgtgatg tggtattttg
      361 aaagtcgtgt atctgaaaat gtatccgtca cctgttttta tagagcgtta ctgaatgtta
      421 caatacttta agttttgcag tcggcaacac gctgtaatac tgctaaatct taggtaaaca
      481 aaagcttata tgtttggagc aagcatcgga cgcggagcaa tgcgaatcat cgacgccctt
      541 gtgcgctggc tcgcttacgg cggcgttcat ccgaacgttt tgaccgttat cggtgtgacg
      601 ataaacgcgg cgtgcggcgt tttgttcggc ttcggcgaat ttttctgggc gggaatcgtg
      661 ctcattgtcg ccaatctttt cgatatgctg gacggcaacg tcgcgcggct gtcgggaaac
      721 gtgacgcgtt tcggcgcata tctcgattcg tcgctcgacc gcctgtcgga tatggtttgc
      781 tttctcggca tcatcgtttt ttacgccagc aacacgcccc agcattcgct tttaaacgtt
      841 tttctggcgg gaatgggaat ggtcggctcg gtgatggtca gctatacgac ggcgcgctcg
      901 gaaagtatgg gcgtgaaggc aaatgtcgga tttttgcagc gaccggaaag aatcgttcta
      961 tttataatcg gcgcgctttc gactcattgg ggatcgacga attttttcgc caaccggatg
     1021 ccgcaggttt tgtgggttct ggcaatcggc tcgttctgga cctttattca tcgaatgtat
     1081 tacacgtgga ttgagctgaa taaaaccgaa atcgctcagg aaaattaaac acgaatggat
     1141 tggctttcag gactttggga ttcgctgacg ctcgaccgga ttttgctttt cggcgggctt
     1201 tttctggcgt cgctcgcttt cagctttatc gtgctcggcg tggtgatggt caaaattccc
     1261 gaagattatt tcagctcgca ttatcagcag aaatttatgc cgaacagctc gtttctggtg
     1321 cgctggggcg cggttatctt aaaaaatctg ctcggcgttt ttctgattat tctcggcatt
     1381 attctttcgc ttccgggcgt tccggggcaa gggctgctga cgattctgct ggggttgata
     1441 atgctcgata taccgggcaa gcgaccgctc gaagcgcgga ttatcaaacg cccgacgatt
     1501 ctggcggcaa tcaataatct gcgcggcaga tttaacaaac ctccgctggt tctcgattaa
     1561 atatggggct tttcagtatc ttcaaacaaa agaaaaacgt cgatttggaa gccgagcggc
     1621 gcgagcgtct gcggcaaaaa ggcagaatct gcgacggcac gattatcgac agcgaaacga
     1681 acgcgcaggg cgaaatcgtt ttttacgtct acagcgttca cggagtcgat ttcgaatctt
     1741 ccgagctttt gagcgaagaa caattaatcg accctttaaa atacgcgccg ggcgcaaagg
     1801 tcggcgttcg cttcgacccg aaaaatcacg ggaattcgat gttagtttag aactgataac
     1861 tgagaactga tagttttatt ttcactatcg gttatcggtt atcggttatc agttaaaatt
     1921 ttatgtttca gaaaatttta atcgccaatc gcggagaaat cgcctgtcgc gtcattcgcg
     1981 cgtgccgcga tatgaaaatt aaaaccgtcg ccgtctattc ggaagccgat aaaaacgcgc
     2041 tgcacgtgcg aatggcggac gaagcttatt ttatcggcgc gccgccgtcg aacgaaagct
     2101 acctgcgctg ggaaaaaatt atcgacgtgg cgaaaaagag cggcgcgcaa gccgtccatc
     2161 cgggctacgg ctttctttcg gaaaacgccg agtttgtccg tcaggtcaac gccgagggaa
     2221 taatatttat cggaccttcg ccggaggcga tggaagcgat gggcggcaaa atctcggcgc
     2281 gaaaaatcgc catcgaagcg ggcgtgccgg ttgttcccgg cacggtcgag cctctgcaat
     2341 cggcgggcga agccgcggaa gtcgcaaaaa aattcggttt tcccgtgatg ctcaaagctt
     2401 cggcgggcgg cggcggcaag ggaatgcggt ttgtcgaaaa cgaaagcgat ttgaaatccg
     2461 cgctggagac cgcgcagagc gaggcgatgg cggctttcgg cgacgcgagc gtgtatgtcg
     2521 aaaaagccat cgtccgcccg cgtcacatcg aaatccagat tttttccgac acgcacggca
     2581 atcacgttca cctgggcgag cgcgaatgct cgattcagcg ccggcatcag aaagttatcg
     2641 aagaatgtcc ttcgccgatt aacgatgcga acttgcgcca aagaatgggc gaatgcgccg
     2701 tgaaagtggc gcgagccgta gattacgtcg gcgcgggaac ggtcgaattt ttagtttcgg
     2761 acgaaacgcg cgagttttat tttctggaaa tgaacacgcg cctgcaagtc gagcatccgg
     2821 tcacggaact ggtgacggga atcgacctcg tgcgcgagca gatacgcgtc gcgtggggcg
     2881 agccgctttc gtttaaacag gaagacgtca cctggaacgg gcacgccatc gaatgccgcg
     2941 tttacgccga agacccggaa aataattttc tgccttcgcc gggcagaatc aatcgcctgc
     3001 gcgtgccgca cggaaacggc gtgcgcgacg acggcggcgt ttacgaaggc gcggaagttt
     3061 cgatttatta cgacccgatg atttcgaaat tcgccgctta cggcaaaaac cgcgaggaag
     3121 cgattgaccg tctgcgccgc gctctggaag aatatgaagt cggcggaatc aaaacgactc
     3181 tgccgttttt tcgcgagatt gtcacggaca cggaatttat cgagggaaat ctcgatacgg
     3241 gttttatttc gcgctggcag gaaaggcgcg cgaaacgcga agcgacggaa attgaaaaag
     3301 atatggctct gattgccgcc gcgctcgctt acacggacaa gcagaaagcg atttcaaagc
     3361 aggcggacgg aaattcgacg ggcgcgagcc gatgggtgat ggctggcaga acggcgatgc
     3421 tgaccaatcg tttataataa ttttttaaga ggaataataa aagatggaag aatttttgac
     3481 acagctgatg ggaaagagaa tcgacatttc gtgcggttcg accgccgtcg tgcgcggcga
     3541 tgtaatggcg gtcaaagacg gagttcttta tttaagagac gaggacgaaa gagtggctta
     3601 tgtcgcgatt gaaaaagttg cgattgtctg ggaagtgaag gaacatcaaa cccgaccggg
     3661 atttgtgggc tgaattattt gcaaaaaacg ggtcgctgat tcaaccggat tatttagcat
     3721 tggttgggat tttatttaaa ttaatcctga ttcatctgaa acaatcctaa aaatccgagc
     3781 cctgtttatc gtcaaataat tgcgcgcttg ccgcaggtgc gagcttttga attaaaaaat
     3841 aaaagagcga gtcctttaaa cgactcgctc ttttattttt tgaatgtaga agtttttagt
     3901 tggttttaac cggcaggcgg cggaagccga cgattcgttt tccccagtaa ccggcgaaag
     3961 gcgaataggt gatgcctttg ctcgaagacg cgtgataaaa gccgttggcg tcggcgacga
     4021 tgcccatatg tccgagcccg ttaaaaaata cgagcgttcc gaatttaaat ctttcgtctt
     4081 cggcgaccgg ctcggaaccc gcccaaaggc tgcgcgcgct cgtccgttca aacgaaaaac
     4141 cagcatctct gaaaacgctc cagacaaatc cggaacaatc ataagtgttc ggtccttccg
     4201 ttccgtagac gtaacgaatg ccgagcttgg cttgaatcga ctgctgcatt ttctgactga
     4261 aagaagggct gtaaacgagg ctgccggcta agtttgcgac cgccggattg accggtctgg
     4321 aatcgacggt ttttctaacg agcggctgct gctgcggttg cgaaggagtc tggctggcga
     4381 cgactatttt attcattaaa gtcggtcgct gcgtaacatt tgattgagtc gtttgcgaag
     4441 gcttcggtgt ggttgtgctc gcgtccggaa tggtgggaac aatgcgcggt ctcgaataaa
     4501 cctgcgcgtc ggcggcgttt gaaaacataa aaagagcggc aaaaaataga aatacacacg
     4561 aaggtttctt ccaaatcatc aataataact ccaggttttt aattttgttt agactcaagg
     4621 agagtttttg cgaacaaggg gaagttcgct gtgtcacaga ctaagaccgt ttggttttgt
     4681 aaaatcgctg aatttggtat ttaaaattcg gttgcgaaac cctgcttatt tttggaattg
     4741 cttgaatggt tttagtaaat gacttaacaa gtttcgccca aaccggagcg aaatgcaaat
     4801 gctaaaatca ttttacctca aaaccggggt cgtttggcaa gagttttctt tgaagcggag
     4861 cgtccgggta atcgccggac atattgtgaa ttgtcaatca taattgtttg accaaagata
     4921 cggaacatca atttagaatt tacaattaat aatttcgctt tctttatttt tgcgcaaagc
     4981 cgcttttgtc agataaaagg gaagcgttaa attacaaaaa agacctgaaa ataattcttc
     5041 aggtctttct cgttttcggc gtt
//
LOCUS       DASWNE010000126         3555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_39480, whole genome shotgun sequence.
ACCESSION   DASWNE010000126 DASWNE010000000
VERSION     DASWNE010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39480"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(255..2330)
                     /locus_tag="VGB68_02640"
     CDS             complement(255..2330)
                     /locus_tag="VGB68_02640"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HEY0048155.1"
                     /translation="MKKISALLLLFSFLISIPLAARADEGMWTFDNPPLRQWKERYNF
                     EPTAEWLEKVRLASVRLNDGGSGGFVSPDGLLVTNQHVADGQLQKLSTKERNLTRDGF
                     YARTRAEELKAPDLEVNVLVSFENVTERVQSSVKTGASDKEAGAQRNAVVSQIEKEST
                     EKTGLKSDVVSLYSGGEYWLYRHKKYTDIRLVFAPEEQIAFFGGDYDNFTFPRHDLDF
                     TFFRAYENGQPAKTPNYFKWSETGPNEGEFVIASGSPGSTARLLTVSQLAYQRDIGNP
                     LQQKVWTLRRNILENYGKRGAEQLRQAGAGIRGFNNSLKRLVGQQEGLQNPRMFGKKE
                     AEEKALKDALAKNAELQKIYAPAWQQIATAYGALPAMSNRLAFSTLSPSRLGSIATQI
                     VRYGEEIKKPNDQRYGEFRDSNLESLKIGLLSPAPIYPEMEEYALTAWLEEGLKTLGA
                     NDPFIKAALGGSTPAEAAKRAVQGTQLKDVAARKALLEGGADAIAKSSDPMIVMARNV
                     EPVIRELRAWNEEKIANVEASAGEKIAKARFAVYGKTIAPDANFNLRIAYGRVAGYEE
                     DTTLVPFKTTFFGLYDRAESFGEKPPYNLPARYKEGRAKLDLSTPLNFVYSADTIGGM
                     SGSPVINRNAELVGLNFDSNLQKLSNRYWYIEEEEGSRAVGVHSAAVLEVLRKLYDAQ
                     DLARELTGK"
     gene            complement(2894..3286)
                     /locus_tag="VGB68_02645"
     CDS             complement(2894..3286)
                     /locus_tag="VGB68_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048156.1"
                     /translation="MKIVALLLVLVFCLALTACSGPVKEANEFMNAKDEVILEMGKKI
                     EASPTEAGVDEARKVFESKKEDLKAKSTVVRDKLKGSHGDLLTKLLDSSISNDKAFNE
                     IRKKVVGNNAADKKFTALQDDFKEAVKY"
BASE COUNT          795 a          893 c          909 g          958 t
ORIGIN      
        1 cgtcgcgccg gacgtcgaag tagacctcga cccgaaaatg tggcgcgccg gacgcgacgt
       61 tcagcttgaa aaagccgtcg aagtaacgct cgcggaaatc aaaagaaacc cgccgcgcaa
      121 accggcgaaa ggcgcttttc cgaattacgc caaaccgtaa ttattaaacg gtaaacggta
      181 aacggtaaac ggtaagttga agttataaaa taacgaactt accgtttacc gtttaccgtt
      241 taaggcgttt cgccttactt gcccgttaat tccctcgcca aatcctgcgc gtcgtagagc
      301 tttcgcaaaa cttcgagaac ggcggcgctg tggacgccga cggcgcggct gccttcttcc
      361 tcctcgatat accagtaacg attcgagagc ttttggagat tgctgtcgaa attcaagccg
      421 accagttccg cgttgcggtt gatgacgggc gagccggaca ttccgccgat cgtgtcagcc
      481 gaatagacga aattcagcgg cgtcgaaagg tctaatttgg cgcgaccttc cttgtagcgc
      541 gccggcagat tgtaaggcgg tttctcgccg aagctttcgg cgcggtcgta taagccgaaa
      601 aatgtcgttt taaaagggac gagcgtcgtg tcttcttcgt agcccgcgac tctgccgtat
      661 gcgatgcgaa gattgaaatt ggcgtcgggc gcgatggttt tgccgtaaac cgcaaaacgc
      721 gctttcgcga ttttttcgcc cgcgctcgct tcgacgttcg cgattttttc ctcgttccag
      781 gcgcgaagct cgcggataac cggctcgaca ttgcgcgcca tcacaatcat cgggtcgctg
      841 gattttgcaa tcgcgtcggc gccgccttca agcaaagctt tgcgcgcggc gacgtctttg
      901 agctgcgttc cctgaacggc tcgtttcgcg gcttcggcgg gcgtcgagcc gccgagcgcc
      961 gctttgataa acgggtcgtt cgcgcccagt gttttcaagc cttcttcgag ccaggcggtc
     1021 agcgcgtatt cttccatttc gggataaatc ggcgcgggcg agagcaatcc gattttgagc
     1081 gattcgagat ttgaatcgcg gaattcgccg taacgctggt cgttcggctt tttgatttct
     1141 tcgccgtagc ggacaatctg cgtggcgatt gaaccaaggc gcgaaggcga aagcgtggaa
     1201 aatgccagtc gattcgacat cgccggaagc gcaccgtaag cggtcgcaat ctgttgccac
     1261 gcgggcgcat aaattttttg cagctccgcg tttttcgcca gagcgtcttt cagagctttt
     1321 tcttcggctt cttttttgcc gaacattcgc gggttctgca agccttcctg ctgaccgacc
     1381 aggcgcttga gcgaattgtt gaatccgcga attccggcgc ccgcctgccg caattgttca
     1441 gcgccgcgct tgccgtaatt ttcgagaatg ttgcggcgga gcgtccagac tttttgctgc
     1501 agaggattgc cgatgtcgcg ctgataggcg agctgcgaga cggtcaaaag gcgcgccgtc
     1561 gagccgggac tgcccgaagc gataacgaat tcgccttcgt tcggtccggt ttccgaccat
     1621 ttgaaataat tcggcgtttt ggcgggctga ccgttttcgt aggcgcgaaa gaatgtgaaa
     1681 tccaaatcgt ggcgcggaaa agtaaagttg tcgtaatcgc cgccgaaaaa ggcgatttgt
     1741 tcttcgggcg cgaaaacgag gcgaatgtcc gtgtattttt tgtgtcgata aagccagtat
     1801 tcgccgccgc tgtaaagcga tacgacgtcg cttttcaacc cggttttttc cgtcgattcc
     1861 ttttcaatct gcgagacgac ggcattgcgc tgcgcgcccg cttccttgtc ggacgcgccg
     1921 gttttaactg acgattgaac gcgctcggtg acgttttcga aagagacgag cacgttgact
     1981 tcgaggtcgg gcgcttttaa ttcttcggcg cgggttcgcg cgtaaaaacc gtcgcgcgtt
     2041 aaatttcttt cctttgtgga aagcttttgc agctgcccgt cagcgacgtg ctggttggtg
     2101 acgagcaagc cgtcgggcga tacgaaaccg ccggagccgc cgtcgttcag gcggacggaa
     2161 gcgaggcgga ctttttccag ccattcggcg gtcggctcaa aattgtaacg ctctttccac
     2221 tgccgcagcg gcggattgtc gaaagtccac attccctcgt cggcgcgcgc cgccagagga
     2281 atgctgatta aaaagctgaa aagcaataaa agggctgata tttttttcat atggtctgct
     2341 ctctccgtaa agaaaacggc gactgcgcga gccttccggc tcattcgcaa ttcccgcgtt
     2401 tgtttttttt agattttaca atttctccga tgtaaatcaa tattacattt ttcgggcgct
     2461 gaggcgaaca gataagatgc aaaaaagcgg tcagaatttg cgggaaaact gataagtgat
     2521 aactgatagt tgatagtctt ttcctggtag tgtcctaatc ttgaggcggc ggaaaattaa
     2581 atattttcaa caactcgata acgtcagtcg aaaagtgtct cataagtcga aaatgttgat
     2641 gagtttcggg attttggtaa tgggacggtt tatgagacga aattgaaaaa tgaggtaatc
     2701 cggttgtcag ccttaaactt tagttactcc aacaattaga gtgtctcaaa aacgtatgac
     2761 ttctgagact ttttttgagc tttttacgaa tctcgaaaac cggcgttttc gagactgttt
     2821 gcagggcgct gccagcgccc tcacgtccgt tgatttgttc ggctggacat tattttttac
     2881 acatactgca cagctaatat ttgactgctt ctttgaaatc gtcctgtaat gccgtaaatt
     2941 tcttgtctgc tgcgttgtta cccactactt ttttgcggat ttcattaaac gctttgtcat
     3001 tacttatgga actatccaac agctttgtca gaaggtcgcc gtgagagcct ttaagcttat
     3061 cgcggacaac agtgctttta gccttcaggt cttctttttt cgactcgaat actttccgcg
     3121 cttcgtcaac cccggcttcc gtcggactgg cttcgatttt tttgcccatt tccagaatta
     3181 cttcatcttt tgcattcata aattcattag cctctttaac cggtccgctg caagccgtca
     3241 gcgccaaaca aaaaactaaa actaaaagca atgcgacaat tttcatatct taattttctc
     3301 ctgtttattt tttcgggttt cccggaacat caatcgaaag agccgaacaa gttattagac
     3361 cgcaaagttg cggtctaaat ccgaaatagg cacaaattac tgctaatatg tgtcaaaaag
     3421 ggtcgctttt gagacaaatc ttagtatagc acacaaaatt aggacactac ctttttcttt
     3481 cactaccagt tatcacttat ctcgtttttg cgcggcggtc attttccacg cgagcagggc
     3541 gatgcaaatc accag
//
LOCUS       DASWNE010000127         2061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_75280, whole genome shotgun sequence.
ACCESSION   DASWNE010000127 DASWNE010000000
VERSION     DASWNE010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75280"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="VGB68_02650"
     CDS             <1..958
                     /locus_tag="VGB68_02650"
                     /inference="COORDINATES: protein motif:HMM:NF025198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HEY0048157.1"
                     /translation="PEDPFKTIVGQPMAEDPFKTVVGGSAAQSKDNDILQLPDESADP
                     MKTMVVAPDDWKKADSSTSAGEMPVPEPPKFNEPNLNPPVFGDLSPQSSGSGGDILGS
                     GGSYSNTPPGEPKISDSPFGQPPSNSPFQSPFEQQKSSFEPPSPFDAPSAPPFDAPKP
                     PPFKEPESPFGNQQTPFDQSPFSQPQSPPGQMGNDPFASQQPQAEWNPPPVPMQNWQD
                     QNLGANTPFQPPPANAGQNNTLAVVSLVLGVLSFLCLGFLGSIPAIITGYMQRNNIKN
                     NPSEYGGGGMAMAGMILGALNLVLNVIFIAIWLMLVLSGGRF"
     gene            1069..1590
                     /locus_tag="VGB68_02655"
     CDS             1069..1590
                     /locus_tag="VGB68_02655"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HEY0048158.1"
                     /translation="MSIISDVINSAKAVIKGMSITLSQIPKEKWTVQYPDEPITVQPR
                     YRGMHLLHVDENGKEKCVACYLCAAACPSDCIYIEAQDDPRPYEERVGRDERYAKVYN
                     IDYGRCIFCGFCVEACPKDAITHGYNFELSVYNRADLLKTKDDLLITKQKQSKNYRVA
                     LSSEDVDSEYKEV"
     gene            1699..>2061
                     /locus_tag="VGB68_02660"
     CDS             1699..>2061
                     /locus_tag="VGB68_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048159.1"
                     /translation="MKCKTFKIRLQADARNHDEAELNKFLESIAVRQSFASIVGDEYW
                     SVLVFYEDAFSGTQSPAQDNFTVQTPAAPAVRQSAAKSVETEKPAPEPIILTPEQENK
                     FNLLKQWRNERANQDGVPP"
BASE COUNT          506 a          566 c          548 g          441 t
ORIGIN      
        1 gccggaagac ccttttaaaa cgatagtcgg gcagccgatg gcggaagacc cgtttaaaac
       61 cgtagtcggc ggcagcgcag cgcagtcgaa agacaacgac attctccaat tgccggacga
      121 gtccgccgac ccgatgaaaa cgatggtcgt cgcaccggat gattggaaaa aagctgattc
      181 gtccacctcg gcaggcgaga tgccggtgcc cgagccgccg aaattcaacg agccgaattt
      241 gaatccgccc gtatttggag atttgtcgcc gcagtcgtcc ggctcaggcg gcgatatttt
      301 aggcagcggc ggctcatatt cgaacacgcc gccgggcgag ccgaaaatca gcgattcgcc
      361 ttttggtcag ccgccgtcca attcgccgtt tcaatcgcct ttcgaacaac agaaatcctc
      421 gttcgagccg ccgtcgccgt ttgacgcgcc gtcagcgccg ccgttcgacg cgccgaaacc
      481 gccgcccttt aaagagccgg aatcgccgtt cggcaatcag cagacgcctt ttgaccagtc
      541 gccgttttcg cagccgcaaa gccctccggg acaaatgggc aacgacccgt ttgccagcca
      601 gcagccgcaa gccgaatgga acccgccgcc cgttccgatg cagaactggc aagaccagaa
      661 tctcggcgcg aatacgccgt ttcagcctcc gcccgccaat gccggacaga ataatactct
      721 ggcggtcgtc tcgctggtgc tgggagtttt gagctttctg tgtctcggtt ttttaggctc
      781 gattccggcg attattaccg gctatatgca gagaaacaat atcaaaaata acccgtcgga
      841 atacggcggc ggcgggatgg cgatggcggg aatgattctc ggcgcgctca atctggtgct
      901 gaacgttatc tttatcgcta tctggctgat gctggttttg tccggcgggc gcttctagta
      961 agagccggct cgtaaaattt tcgccgctaa aatgtttaaa gcctgcttcg attggaatga
     1021 ggcaggcttt tcaagttata atattgtgaa aacttttaca aaaaaaatat gtcaataata
     1081 agcgatgtca tcaactcggc aaaagccgtg ataaagggaa tgagtattac gctctcacaa
     1141 attccgaaag aaaaatggac ggtgcagtat ccggacgagc ccatcaccgt tcagccgcgt
     1201 tatcgcggaa tgcatctgct tcacgtcgat gaaaacggca aggaaaaatg cgtggcgtgt
     1261 tacctgtgcg cggcggcttg cccgtccgat tgtatttata tcgaagcgca ggacgacccg
     1321 cgcccgtatg aagagcgcgt cgggcgcgac gagcgctacg cgaaggttta caacatcgac
     1381 tacgggcgct gcattttctg cggcttctgc gtcgaagcgt gtccgaaaga tgccatcacg
     1441 cacggctaca actttgagct ttcggtttat aaccgcgcgg atttgctgaa aaccaaagac
     1501 gatttgctta tcaccaagca aaagcagtcg aagaattacc gcgtcgcttt atcgagcgaa
     1561 gacgtcgatt cggaatacaa ggaagtttaa aatagtgagc agtaagcggt gagcggtgag
     1621 cagccgggcg tttcggaatt cgacgttaag ttttaaaatc ctgccgctca ccgcttactg
     1681 cttacggctc actgctttat gaaatgcaaa actttcaaaa tccgccttca ggcagatgcg
     1741 cgaaatcacg acgaggcaga gctcaataaa tttctggaat caatcgccgt ccgccagagc
     1801 ttcgcttcaa tcgtcggcga cgaatactgg tctgttctag tcttttacga agacgctttt
     1861 tccggcactc aatctccggc tcaggacaat tttacggttc agacgcctgc cgcgccggcg
     1921 gtcaggcaat ccgccgccaa aagcgtcgaa accgaaaagc ccgcgcccga accgattatc
     1981 ttaacgcccg aacaggaaaa taaatttaac ctgttgaaac agtggcgcaa cgaacgcgcc
     2041 aaccaggacg gcgtgccgcc g
//
LOCUS       DASWNE010000128         6286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_604668, whole genome shotgun sequence.
ACCESSION   DASWNE010000128 DASWNE010000000
VERSION     DASWNE010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6286
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604668"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VGB68_02665"
     CDS             <1..424
                     /locus_tag="VGB68_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048160.1"
                     /translation="DNVIYESNSLGYVNATHQKGIDHGQTVFTYYFPMCSTDGRTKLF
                     AADWRELADICLTDLSLAHKNIRELTRRIDIMRWGHAMISPRTNFLWSGARDEAQKPF
                     RNIHFAHSDLSGIALFEEAFYHGIRAAGEILEKNIYKD"
     gene            447..866
                     /locus_tag="VGB68_02670"
     CDS             447..866
                     /locus_tag="VGB68_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048161.1"
                     /translation="MEYYVPVEDRYLSIKGKVSQSSFDLIKSGLPADKPNNAEILFVG
                     YAFADVPVGKRFSVIFPKNNPGEGIYYESKIVAVTQQFGKPTEVIPRGWKTICLVEFP
                     QGIPEIIKNLPVVDTWFETNDYVCISDEEAWEAIKKL"
     gene            873..1925
                     /locus_tag="VGB68_02675"
     CDS             873..1925
                     /locus_tag="VGB68_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048162.1"
                     /translation="MSEALPQQKWLFSAPVDLTVFLGSAILSLLLLAVGWQLGVLHDE
                     SPDWTWITAVLLIDVAHVWSTSFRVYFDTEEFKKRIWLYTLVPLFGYLVGVALYSESA
                     LTFWRVLAMVAVFHFVRQQYGWVNLYRAKLKEKSRITRRIDAAAIYLATVYPLAFWMT
                     SLPRNFEWFVKDDFFALPVLVEKILFPVYASALVVYFGKSVYLYFKDGFLNIGKDIVV
                     ATTAVCWYVGIVALDSDYAFTVTNVIIHGVPYFALVYFYARSRRENAPRFYQALSGNW
                     IVFLATLWALAYVEEIFWHRGVWHERNWMFGANFDTENWKTYLVPLLAVPQLTHYVLD
                     GFVWRRKYNSNFRLIN"
     gene            1946..2584
                     /locus_tag="VGB68_02680"
     CDS             1946..2584
                     /locus_tag="VGB68_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048163.1"
                     /translation="MKRLIYAEDQREIKKGFSYCLFCLSLLIFLFSACQKNNNSDSSG
                     IDVFGLTDQTAEAGAVIQDANEDLKKIRKMYKENQSRIDELKTAMNDKKIDEVKKIAD
                     ELVYIINDGFVLGESAISKIEKAEAMNTNDKYKEYLQLKKGSLKKQLDAFEFRRQAAQ
                     LLRDAFGNKDKLEIEKAKSEFREKEENFQKYMEIAREMSGEANQLAKETLQQ"
     gene            complement(2699..3754)
                     /locus_tag="VGB68_02685"
     CDS             complement(2699..3754)
                     /locus_tag="VGB68_02685"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY0048164.1"
                     /translation="MLRKLSAFILCATSSCAVIYSQKTPEAPRQPEKQQRAQAMVFSE
                     FFGGSYLGVQTQEITRENFSKFGLNEVRGVGVEKVLENSPAAQAGLQSGDVIVRFEGE
                     EITSTRKLSRLIAEVAPDHQARLTILRGGSERELTVTVGKRPTPKFEDGTFEMRVPPV
                     AGEMPLRIPTPRIPPAGEMPQQLKTIPPVGELNRDFLVFRGSGRQIGAGLMPLTKQLA
                     EYFGINEGRGLLINDVRPDSPAAKAGLKAGDVIIEIEGKEVKGMGDFIRALSEKKEGD
                     VTLTFVRDRNRQTVRVTPEKMKGGGEFFFEFDDKDGLLRTPLPTVPVMPEAPGVKGLL
                     SPTTLPAVPVFAPTRVL"
     gene            3993..4649
                     /locus_tag="VGB68_02690"
     CDS             3993..4649
                     /locus_tag="VGB68_02690"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF009668.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY0048165.1"
                     /translation="MAIRLGDEAPNFTAETTEGTINFHEYLGDGWGILFSHPKDYTPV
                     CTTELGMAARLKPEFDRRNVKIIGLSVDALDAHEGWSKDIEETQGAKVNFPMIADADK
                     KVADLYDMIHPNASDTATVRSVFVIGPDKKVKLNLTYPASTGRNFDEILRVIDSLQLT
                     AKHSVATPANWKDGDDCIIVPTLSNEEAKEKFPAGWKEVKPYLRITPQPNKEAKSEGV
                     "
     gene            4703..5497
                     /locus_tag="VGB68_02695"
     CDS             4703..5497
                     /locus_tag="VGB68_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048166.1"
                     /translation="MISKLLILLLIFNFGLISLSCKALSGLTRKSGTVFTVEIEPDEP
                     HRREIIEQAMRITESKLNSIKADGEVARVPGKDNQISVKIYDSNNTAALKKFLFTSYR
                     LELKKVVSPPYPAPVQTYPTEQAAVPVAAGDREVLSYPDSEDSAFSQFVTVENKSVIN
                     GEDVSDAEAFSRSGSKNDYAIYFTLNPKGAAKLSDWTSKNINNYIAVILNKEVKSIAF
                     IKSQISDNGEIAGRFTEEEAENIALSLKSGYLPATMKIIEEKTFQN"
     gene            complement(5494..>6286)
                     /locus_tag="VGB68_02700"
     CDS             complement(5494..>6286)
                     /locus_tag="VGB68_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0048167.1"
                     /translation="HVPLYQTKDGWHFEKDELRNAFNEKTKAIIICNPNNPTGKVFSR
                     EELEFIAELCREYDAICFTDEIYEHILYKGKREAGQMTTDNPKHISMAQLDGMRERTV
                     VVNSLSKTYSVTGWRVGYCIAPPDISGAIRKVHDFLTVGAANPLQHAGAYALSLPESY
                     YARLQKEYQRKRDFIVPVLQEAGFKCDLPQGAYYVMTDISDFGFESDIEFTKHLIREI
                     GVTVVPGSSFYHDTSLGSQKVRFCFCKKDETLEAAAERLRKLKKG"
BASE COUNT         1625 a         1531 c         1568 g         1562 t
ORIGIN      
        1 ggacaacgtg atttacgaaa gcaactctct cggctacgtc aacgcgacgc atcagaaagg
       61 aatcgaccac ggtcagacgg ttttcacata ttatttcccg atgtgctcga ccgacggcag
      121 aacgaaatta tttgccgccg attggcgcga gctcgccgat atctgcttga cggatttgtc
      181 gctggcgcac aaaaatattc gtgagctgac ccggagaatc gacattatgc gctggggaca
      241 cgcgatgata agcccgcgca caaatttttt atggagcggc gcgcgcgacg aggcgcaaaa
      301 gccttttaga aacattcatt tcgcgcattc ggatttgagc ggcatcgcgc ttttcgagga
      361 agccttttat cacggaatcc gcgcggcagg tgagatttta gaaaagaata tttataagga
      421 ttgaaactgg tttcaagata atctttatgg aatattacgt gccggtcgaa gaccggtatc
      481 tttcgataaa aggcaaagtc tcgcaaagca gtttcgattt gattaagtcc ggtttgccgg
      541 ctgacaagcc gaataatgcc gaaattttat ttgtcggcta tgctttcgcc gatgttccgg
      601 tcggaaaaag attcagcgta atctttccga aaaataatcc gggcgaagga atttattatg
      661 aaagcaaaat tgtcgccgta actcagcaat tcgggaaacc gacggaagtt attccgcgcg
      721 gctggaaaac gatttgtctg gttgaatttc cgcagggaat tcccgaaatt ataaaaaatc
      781 ttccggttgt cgatacctgg tttgaaacaa acgattatgt ttgtatcagc gacgaggaag
      841 cctgggaagc aatcaaaaaa ctataatatt tgatgagcga agcattgccg caacaaaaat
      901 ggttatttag tgcgccggtc gatttgaccg tttttctcgg cagcgcgatt ttgtcgcttt
      961 tgcttcttgc ggtcggctgg cagttgggcg ttttgcacga cgaatcgccg gattggacct
     1021 ggataacggc tgttcttttg attgacgtgg cgcacgtctg gtcgacgagt tttcgcgttt
     1081 atttcgacac ggaggaattc aaaaaaagaa tctggcttta cacgctcgtg ccgcttttcg
     1141 gttatctggt cggcgtggcg ctttattcgg aatcggctct gactttctgg cgcgtgctgg
     1201 cgatggtcgc ggtttttcat tttgtccggc agcaatacgg ctgggttaat ctttaccgcg
     1261 cgaaattgaa ggagaaatct cgaataacgc ggcggattga cgcggcggcg atttatctgg
     1321 cgaccgtcta cccgctggcg ttttggatga cgagcctgcc gcgcaatttc gaatggtttg
     1381 tcaaggacga ttttttcgct ttgcccgttt tagtcgaaaa gattctcttt ccggtttacg
     1441 cgtcagcgct ggttgtttac ttcggaaaat ccgtctatct ttatttcaaa gacggctttt
     1501 tgaacatcgg caaagatatc gtcgtggcga cgaccgccgt ttgctggtat gtgggaatcg
     1561 tcgcgctcga ttccgactac gcttttacgg tgacgaacgt gataattcac ggcgttccgt
     1621 attttgcgct cgtttatttt tacgcgcgct cgcgccggga aaacgcgccg cgtttttatc
     1681 aagcgctttc cggcaactgg attgtctttc tggcgacgct ctgggctctg gcttacgtcg
     1741 aggaaatttt ctggcatcgc ggcgtctggc acgagcgaaa ctggatgttc ggcgcgaatt
     1801 tcgatacgga aaactggaaa acgtatctcg tgccgctgct cgccgttccg caattgacgc
     1861 attatgtgct ggacggcttc gtctggcgtc gcaagtataa ttcaaatttt cgcctgatta
     1921 attagatttt taacccgaaa gaaaaatgaa acggctaatt tacgcagaag atcaacgcga
     1981 gattaaaaaa ggattttcat actgcttatt ctgcctgtcc ctgttaattt ttcttttttc
     2041 cgcgtgccag aaaaataata attcggattc gagcggcatc gacgttttcg gtttgaccga
     2101 ccagacggcg gaagcgggcg ccgtcatcca ggacgcgaac gaagacctca aaaaaatccg
     2161 caaaatgtat aaggaaaatc agagccgcat tgacgagctg aaaacggcga tgaacgataa
     2221 aaaaatcgat gaagtgaaaa aaatcgccga cgaactggtt tatatcatca acgacggttt
     2281 cgtgctgggc gaaagcgcga tttccaaaat cgaaaaagcc gaggcgatga acaccaacga
     2341 taaatacaag gaatatttac agttaaaaaa gggaagcttg aaaaaacaac tcgacgcttt
     2401 cgagtttcgc cgtcaagccg cgcagctttt acgcgatgct ttcggcaaca aagacaaact
     2461 ggaaatcgaa aaagcgaaat cggaattcag ggaaaaagaa gagaattttc agaaatatat
     2521 ggaaatcgcc cgcgagatga gcggcgaggc gaaccagctt gccaaggaaa ctttgcagca
     2581 gtaaaaaaac ggaaaaacga ttaacttcgc ttttccgttt ttttatagac cgatttttta
     2641 ctgcacgtat gccgccgcag agtttccgca ccggctaagg cgcgggcttc agcctttttt
     2701 acagcacacg tgtcggagcg aaaaccggca cagccggtaa agtcgtcggc gacaacagtc
     2761 cttttacgcc cggcgcttcc ggcataaccg gaaccgtcgg cagcggagtt ctcaacaacc
     2821 cgtccttgtc gtcaaactca aagaaaaact cgccgccgcc tttcattttt tccggcgtga
     2881 cgcgcacggt ttgacgattg cggtcgcgga caaaggtcaa agtcacgtcg ccttctttct
     2941 tttcgctgag cgcgcgaatg aaatcgccca ttcctttgac ttctttgcct tcgatttcga
     3001 ttatcacgtc gccggctttc aaaccggctt tggcggctgg cgaatccggg cgaacgtcgt
     3061 taatcaaaag tcctctgcct tcgttgatgc cgaaatattc agccagttgt ttggtcaaag
     3121 gcatcagccc cgcgccgatt tgtctgccgc tgccgcgaaa aaccagaaag tctctgttca
     3181 gctcgcccac cggcggaatc gttttgagtt gctgcggcat ttcgccggcg ggcggaattc
     3241 gcggcgtcgg aatgcgcagc ggcatttcgc cggctacggg cggaacgcgc atctcgaaag
     3301 tgccgtcttc aaatttcggc gtcggacgct ttccgaccgt gacggtaagc tcgcgctcgc
     3361 tgccgccgcg cagaatcgtc agccgtgcct gatggtcggg cgcgacttcc gcaatcaggc
     3421 gcgataattt acgcgtgctg gtaatttctt cgccctcgaa acggacgata acgtcgccgc
     3481 tctgcaatcc ggcttgcgcc gccggcgaat tttccagcac cttttcgacg ccgacgccgc
     3541 gaacttcatt caatccgaac ttcgagaaat tctcgcgcgt tatttcctgc gtctgcacgc
     3601 cgagataact gccgccgaaa aactcggaaa acaccatcgc ctgcgcccgt tgctgttttt
     3661 cgggctggcg cggcgcttcc ggcgtttttt gcgaataaat aacggcgcag ctcgaagtcg
     3721 cgcacagaat aaaagcggat aactttcgta acataaaacc tccagatttt agaatttttt
     3781 ctaatggaat tttcgggagc tttccctcgt ttaaatacct tacgaattac ttatgattta
     3841 ggttgcacga gatattcaaa aagttgtacc ggcgcccggt gcgggacttg aaaaacctct
     3901 gccgaacgca taccattatt aaacttccaa attattattt aactattaaa gctcgtaaat
     3961 tacggcttta aaaatcgaag aggagcaaaa aaatggcgat acgcttagga gacgaagcac
     4021 cgaattttac ggcggaaacg accgaaggaa caatcaattt tcacgaatat ctgggcgacg
     4081 gctggggcat tttgttctcg catccgaagg attacacgcc cgtctgcacg accgaactgg
     4141 gaatggcggc gcggctgaaa ccggaattcg accggcgcaa cgtaaagatt atcggcttga
     4201 gcgtcgacgc gctcgacgcg cacgaaggct ggtcgaaaga catcgaggag acacagggcg
     4261 cgaaggtaaa tttcccgatg attgccgacg ccgataaaaa agtcgccgac ctttacgata
     4321 tgattcaccc gaacgcgagc gataccgcta ccgtgcgctc ggttttcgtc atcggacccg
     4381 acaaaaaagt gaaactgaat ttaacttatc cggcgagcac cggacgaaac ttcgacgaga
     4441 tactgcgcgt catcgactcg cttcagctga cggcgaaaca cagcgtcgcc acgccagcca
     4501 actggaagga cggcgacgac tgcatcatcg ttcccacgct gtcgaacgag gaagcgaaag
     4561 aaaagtttcc ggcaggctgg aaagaagtga agccttacct gagaattacg ccgcagccga
     4621 ataaggaagc gaaaagcgaa ggcgtataaa ctaaaaggct ttgcaagtta atttattgca
     4681 aagccttttt ttctaaaatt ctatgatttc aaaactcctg attttgttat tgatttttaa
     4741 tttcggttta atcagcttaa gttgtaaggc tttatcgggt ttaactcgga aaagcggaac
     4801 ggtttttacg gttgagatag aacccgacga gccgcatcgc cgcgaaatta tcgaacaggc
     4861 gatgagaatt accgagtcga aactcaattc aataaaggcg gacggtgagg tcgcccgcgt
     4921 tcccggcaaa gataatcaga tttcggttaa aatttacgat tcgaataata cggcagcgct
     4981 gaaaaagttt ttatttacga gctaccggct ggaattgaaa aaagtggttt cgccgccgta
     5041 tcccgcgccc gtccaaactt atccgaccga gcaagccgcc gtaccggtcg ctgccggaga
     5101 ccgggaagtt ttatcctatc cagacagtga agattctgcc ttttcgcaat ttgtaacagt
     5161 cgagaacaag tctgtcataa acggcgaaga tgtcagcgac gcggaagctt tttcaagaag
     5221 cgggtcaaaa aacgactatg cgatttattt cacgctcaat ccgaaaggcg cggcaaagct
     5281 cagcgactgg acgagcaaaa atataaataa ttatattgcc gttatactga acaaagaagt
     5341 taaaagcatc gctttcataa aatcgcaaat ttccgacaac ggtgaaattg ccggcagatt
     5401 taccgaagag gaagccgaaa acattgcgct gagcctgaaa agcggctatc tgccggcgac
     5461 gatgaaaata atcgaagaaa agacctttca gaattaacct tttttcaatt tccgcaatct
     5521 ttccgccgcc gcttcgagcg tttcgtcttt tttgcagaaa caaaaccgaa ctttttgcga
     5581 gcctaagctc gtgtcgtgat aaaacgacga acccggaacg accgtcacgc cgatttcacg
     5641 aatcagatgt tttgtaaatt caatgtccga ttcaaaaccg aaatccgaaa tatcggtcat
     5701 cacgtaataa gcgccctgcg gcaaatcgca tttaaaaccg gcttcctgca aaaccggcac
     5761 gataaaatcg cgctttcgct gatattcctt ttgcagcctg gcgtaatagc tctccggcag
     5821 gctcaaagca tacgcgcccg cgtgctgcaa aggattcgcc gcgccgaccg ttaaaaaatc
     5881 gtgcactttg cgaatcgcgc ccgaaatatc cggcggcgcg atgcagtagc cgacgcgcca
     5941 gcccgtgacc gaataagttt tcgacagcga attgacgacg acggttcttt cgcgcattcc
     6001 gtcgagctgc gccatcgaga tatgtttcgg attatccgtc gtcatttgtc cggcttcccg
     6061 cttgccttta taaagaatgt gctcgtaaat ctcgtcggta aaacaaatcg cgtcgtattc
     6121 cctgcagagc tcggcgataa attccaattc ttcgcggctg aagacttttc cggtcggatt
     6181 gttcgggttg cagatgatga tcgctttcgt cttttcgtta aaggcgtttc tcaattcgtc
     6241 tttttcaaaa tgccagccgt ctttcgtttg ataaagcggg acgtgg
//
LOCUS       DASWNE010000129         3588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_674695, whole genome shotgun sequence.
ACCESSION   DASWNE010000129 DASWNE010000000
VERSION     DASWNE010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674695"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..582)
                     /locus_tag="VGB68_02705"
     CDS             complement(133..582)
                     /locus_tag="VGB68_02705"
                     /inference="COORDINATES: protein motif:HMM:NF013583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HEY0048168.1"
                     /translation="MTEIQENAKEFLVEFLKDFRFDLNVSAEKIDEGYYFDLTGDDVP
                     FLLNESGELLDALEVLLFQIFGKDIDRTERFICDADGFRQTRKAELQAMARFAAQNVR
                     KSGNPFTFGKLNSTERRVIHLTLQTETDLTTESVGEGRDRRLKVQLK"
     gene            complement(585..2474)
                     /gene="yidC"
                     /locus_tag="VGB68_02710"
     CDS             complement(585..2474)
                     /gene="yidC"
                     /locus_tag="VGB68_02710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEY0048169.1"
                     /translation="MSDNKQSVQQRFLVAAVLSLLVLTVWSYFFAPPKPKDNANTASN
                     VAATNTPAPETVQTQPTPAIVNAPDNIPNRIITIKSPLYEVKLDAKGAVATSWILIKN
                     VHDHHEKALFSNDSTEANPKPLELISPEALNRNPREIPFRLSTGDQNLDAYLNDRNYQ
                     VSQTEDTIQLKDSESKQIDFVLKDESGLEVTKSFVFRADSYLTDLAIKAARNGQPVPN
                     TKLLIGASIGDQGIIHHNYYHIEPEAVSFVNGSIQRYPGASFTYVNNEAVKPFEGAVD
                     WAGVGDTYFAMAAVPAQQLPGLEYRALKYEVQTEPFYDGIISWITRSQSTKLTKHLVS
                     TYVPVTTDGSVTRIYAGSKDYFTLAEYNKNLTAAGVGRTVEIEELINFGWLEPITRPI
                     AIPLLRALNFIYGFVGNYGVAIIIFTLIFYSLFFPLRWYQSKSFKKAQASAPRMKEIQ
                     DRLKDLQKKGVPMDDPRMREAQMEQLKMMKGAVPIGGCLPLLLQMPLFIALYVAVTIS
                     LDFRQASFLWLPDLSGGDPWHLLEFLFAASMAGSMILTPTTAAITPEQQMQQKMMTYM
                     MPLMMLWVMWGSPAGLLLYWFFGNIVSFVQQFIINRLNKTNEPPKEEVVEVVSKKAKP
                     KFSTP"
     gene            complement(2574..2783)
                     /gene="yidD"
                     /locus_tag="VGB68_02715"
     CDS             complement(2574..2783)
                     /gene="yidD"
                     /locus_tag="VGB68_02715"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEY0048170.1"
                     /translation="MKFLFLNFLKLYKALLSPFLPPACRFTPTCSAYAMEAVEKYGAF
                     RGTWMSIKRILRCQPFCKGGYDPVK"
     gene            complement(2780..3211)
                     /gene="rnpA"
                     /locus_tag="VGB68_02720"
     CDS             complement(2780..3211)
                     /gene="rnpA"
                     /locus_tag="VGB68_02720"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEY0048171.1"
                     /translation="MNAASLLNFGLPKDSRLRKSAEFRRVYAEGKRFDGRFMTVFVLP
                     SKTESHKLGITASKKGIGKAFERNRAKRLLREAFRLSRVELSDLKGRYDWVLNARRRL
                     LQVKLEKPLEDFRQIIEAIKNSESELNQGERNAAVGEVQKR"
     gene            complement(3190..3336)
                     /gene="rpmH"
                     /locus_tag="VGB68_02725"
     CDS             complement(3190..3336)
                     /gene="rpmH"
                     /locus_tag="VGB68_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEY0048172.1"
                     /translation="MPKRTYQPNNRRRAKKHGFRARMATKNGRAVLARRRAKGRKRLT
                     PHHY"
BASE COUNT          938 a          872 c          813 g          965 t
ORIGIN      
        1 taaacgaata acggcaagac cgcttcgacc gagaggtgta gctaatgcga caatcgtatc
       61 cataaatcat aaagaaaaag gcaaagggaa aagatttcct tcgcctttac cacaaaaatc
      121 gttttccgta atttatttta gctgaacttt caggcgtctg tccctgccct cgccgactga
      181 ttcggtggtt aaatccgtct cggtttgtaa agttaaatga attacgcgcc tttcagtcga
      241 gtttaacttg ccgaatgtaa acgggttacc ggatttcctg acattctgag ccgcaaatcg
      301 cgccattgcc tgcaattcgg ctttcctggt ttgccggaaa ccgtcggcat cacaaataaa
      361 tcgctcggtt ctatcaatat ccttaccgaa aatctgaaac agcaaaactt ccagcgcgtc
      421 cagcaattcg ccgctttcgt tcaacaaaaa agggacgtca tcgccggtca ggtcaaagta
      481 ataaccttca tcaatcttct ccgccgagac gttcaggtca aagcgaaaat cttttaaaaa
      541 ttcaaccaaa aattctttgg cgttttcctg tatttcagtc atttttacgg tgtcgaaaat
      601 ttcggtttag ctttttttga aactacttca acaacttctt ctttcggcgg ctcgttagtt
      661 ttattcaggc ggttaataat aaattgctga acaaaactca caatattacc gaaaaaccag
      721 taaagcagaa gtccagccgg cgagccccac atgacccaga gcatcattag aggcatcata
      781 taggtcatca ttttctgctg catctgctgt tcgggcgtta ttgccgcggt cgtcggcgtt
      841 aaaatcatcg agcccgccat cgaagcggca aaaaggaatt cgagcaaatg ccacgggtcg
      901 cctcccgaaa ggtccggaag ccacaaaaat gatgcctggc gaaaatcgag cgaaatcgtt
      961 acggcgacgt aaagcgcaat aaacagaggc atttgcagga gcaaaggcaa acagccgccg
     1021 atgggaaccg cgcccttcat cattttcagc tgttccatct gcgcttcgcg catccgcggg
     1081 tcgtccatcg gaacgccttt tttctgcaaa tctttcaggc ggtcttgtat ctctttcatt
     1141 ctcggcgcac tagcctgcgc ttttttgaag gatttcgatt gataccagcg aagcgggaaa
     1201 aacagcgaat agaaaatcaa ggtaaaaatg attatcgcca cgccgtaatt gccgacaaaa
     1261 ccgtaaatga agttcaacgc ccgcaaaagg ggaatcgcta tcggacgggt aatcggttca
     1321 agccatccga aattgattag ctcttcaatc tcgaccgttc tgccgacgcc ggcggcggtc
     1381 agatttttat tgtattcggc gagcgtaaag taatctttcg aaccggcata aattcttgta
     1441 accgagccgt ccgtcgtaac cggaacataa gtcgaaacca gatgtttagt cagtttcgtc
     1501 gattggctgc gcgtaatcca cgagataatg ccgtcataga aaggttcggt ctgaacttca
     1561 tattttaagg ctcgatactc gagtccggga agctgctgcg ccggaactgc cgccatcgca
     1621 aaatacgtat cgccgactcc agcccaatct accgctcctt caaaaggctt taccgcctca
     1681 ttattgacgt aagtaaaaga cgctcccgga tagcgctgaa tcgagccatt tacaaagctg
     1741 accgcctccg gttcgatatg ataataatta tggtgaataa tgccctggtc gccgatgctg
     1801 gcgccgatta aaagcttcgt gtttggaacg ggttgaccat tgcgcgcggc tttaattgct
     1861 aaatcggtca gataactgtc ggcgcggaaa acgaagcttt ttgtcacttc cagaccgctt
     1921 tcgtctttta aaacaaaatc gatttgtttc gattccgaat ctttaagctg gatagtgtct
     1981 tcagtttgag aaacctgata gtttctatca ttcaaataag catccagatt ctggtcgccg
     2041 gtcgaaagcc ggaaaggaat ttcgcgcgga ttgcgattta acgcttccgg cgaaatcaac
     2101 tccagcggct tcggattagc ttcggtcgaa tcattggaaa aaagagcttt ttcgtggtgg
     2161 tcgtgaacgt ttttaatcaa aatccagctg gtcgcgaccg caccttttgc atcaagtttt
     2221 acttcgtaaa gcggtgattt aattgttata atccggttcg gaatgttatc aggagcatta
     2281 acaattgcgg gcgtcggttg agtttgaacg gtttcaggcg ccggcgtatt cgtcgccgct
     2341 acattcgacg ccgtattggc attatctttc ggcttcggcg gagcgaaaaa atagctccaa
     2401 actgtcagca caagcagcga aagcaccgct gcgactagaa atctttgttg aactgattgt
     2461 ttattatctg acatttttaa attttcgatt ttcgattttc gattttcgat ttggaaagaa
     2521 ttcaagactt aaatttctca ttcaatcgca aatcggcaac cgcaaatcga aaatcacttc
     2581 accggatcgt acccgccttt acaaaacggc tgacaacgta agatacgttt tatgctcatc
     2641 caggttccgc gaaatgcgcc gtatttttcg acggcttcca tcgcgtatgc cgagcaggtc
     2701 ggcgtaaaac ggcacgcagg cggcaaaaaa ggcgacagca acgccttgta aagcttcaaa
     2761 aaattcaaaa acagaaactt catcgttttt gaacctcacc gacggcagcg tttctttcgc
     2821 cctgatttaa ttccgattca ctgtttttaa tcgcttcgat aatctgccgg aaatcttcca
     2881 aaggcttttc gagcttgacc tgcagcaatc ggcgccgcgc gttcaaaacc caatcgtatc
     2941 tgccttttag gtcgcttaat tcgacgcggc tcaggcgaaa cgcttcgcgc aaaagacgct
     3001 tcgcgcgatt tcgctcgaaa gccttgccga ttcctttttt cgatgccgta attccgagtt
     3061 tatgcgattc ggttttcgaa ggcaaaacaa agacagtcat aaaacgaccg tcaaaacgtt
     3121 ttccctccgc ataaacgcgg cgaaattcgg cagatttccg caaacggctg tctttcggca
     3181 gcccgaagtt taataatgat gcggcgttaa tctcttgcgc ccttttgccc ggcggcgcgc
     3241 taaaaccgct ctgccgttct tggtcgccat tctggcgcgg aagccgtgct ttttcgcccg
     3301 gcgacgattg tttggttgat aagttctctt tggcatatct gttcatcctt tcacgtcgaa
     3361 gtgaaaaggg ttagtttaat agtttcgggc gattatgtca aaaaccggag cgaggcaatt
     3421 acaatttttc gaaaatcgta aagtgaaatc gcacttgctt ccgggcggtt ggtgaaggga
     3481 aattgaggct tcgccgatta caatcgtccg ggtaagggta attgccgcgt ttatattccg
     3541 gtttctttaa aagggttgag aaacgaaagg gaaatctccg tacccggc
//
LOCUS       DASWNE010000130         5401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_251845, whole genome shotgun sequence.
ACCESSION   DASWNE010000130 DASWNE010000000
VERSION     DASWNE010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5401
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251845"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..1213)
                     /locus_tag="VGB68_02730"
     CDS             complement(71..1213)
                     /locus_tag="VGB68_02730"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY0048173.1"
                     /translation="MAKTKSFLYPMLLFFSLGIGCSIKTPAKNSISGEIKSLPAGTLQ
                     LILEEDVNRKKSKIIAEIPIDGDGRFKFEKELPPHIYSLKINDKKSVMLAVEKGQRII
                     ITGDAADSGRLQVTGSEDTAKLEAYEKFRKESLNRLVISIRNQIKELKEKGAPENDSK
                     LLELAGLEIENYDRHKDELIEFIKKEMGTSIAVYPTSIRWDGEKNIPFLIELAGQFES
                     NHPNTEIAARINEKVKAITANRLGGRVAEIKMPDKNGEIVSLSSINAKYILIDFWGSW
                     CLPCRRESRLLGELYQKFKPEGFDIYGVGLESEKENWLKAIEQDKRIWTNVSTFQEFE
                     TPVTFDYAVTSLPANVLVDNSGKVIAKNLHGNALKATIEKLFSTEK"
     gene            complement(1242..2720)
                     /locus_tag="VGB68_02735"
     CDS             complement(1242..2720)
                     /locus_tag="VGB68_02735"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY0048174.1"
                     /translation="MNRNSTLTLVFVLLVLAAGGAARGQDFKTDVWSEDFAQLKREMS
                     ASYANLEWAVENRGLDLKQLSGQTESRLLGARSEPEAKKIIESFLDAFGDGHLSVQWA
                     QNKVTNSSPADNAQPNSQMPLCRRLGYQSQNQTPRIAFTRLDNFREINTEDSKYFSIG
                     VLRAAAQSDVGVIRIAIFSEYAFPDLCEQAAAQMSLTKDSPCDAECEDKVERRAANLL
                     TAALARQIEALKRNRINALLADITGNGGGTNWTEPAARTLSDKPLRSPRQQFIRHEHW
                     TKQLKRRLEIIEADARQSASPNRELLLQGENILRKAISESQQPCDRGGVWENRKPDCS
                     LVVTAPPLYPQSVLSFAEPGSLPDKPSSRFLFYPSRYAYREGVYAGRLMVLVDQGTWS
                     SAEYFAAMLQDNNAATIIGEPTGGAGCGYTNGGIQTYLKNSRAGVKIPDCVRLRADGT
                     NEVAGITPDVLIAWRSNDSRYQRVKRVSDALSEHMNGKTKNR"
     gene            complement(2848..3963)
                     /locus_tag="VGB68_02740"
     CDS             complement(2848..3963)
                     /locus_tag="VGB68_02740"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HEY0048175.1"
                     /translation="MKNYLFLLAIVLFAVPLYAQVPYNSAVPNGYTKKAAVSEQVGLT
                     QVTITYHRPAVKGREGKIWGGIVHKGFVDQGFGNGKPAPWRAGANENTVIEFDNDVKI
                     EGQTLPKGKYGLFIAYDPAESTVIFSKRSDGWGSFFYDEKEDALRVKIKPQPIEKSVE
                     FLKYEFSDSTPNSAVITLAWEKLSMPFKVEGDYLKQQFDAFVAESQNPRSFTSQSLNI
                     AASWTLQNNYQLEKGLEWATAATGSKFPGDPTSFPALSTKAMILDKLGRTDEAAAVIK
                     SALPFGGVVQLQQFGRQLLNAKKPQAALEVFQFNYDKNPNQFITLTGMARGLSANGEY
                     AKALEFASKALPLAPNDAGKQAVQAMIELLKAGKDIN"
     gene            complement(4262..>5401)
                     /locus_tag="VGB68_02745"
     CDS             complement(4262..>5401)
                     /locus_tag="VGB68_02745"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEY0048176.1"
                     /translation="PVGVSGPIRFNFNYTGGTTGTIGPLTFTTTPAQVAELRSNKWYV
                     NIHTANFPNGEIRGQVKRANIPADYDGDGRSDLVVFRQSNFTFYTLNSLENNLTTSIE
                     VGRGLDDLPGIVNNLGDFDGDGRADRVAWNLDDNNVIRWFILQSETNSIRVLDWGQFA
                     PTGSDAFVPADYDGDGKLDVAVMRRATGVWYIIESSTGNPRYEYWGTTNDAPAVGDYD
                     GDGKADLCVTRVENGLRYWYIRNSSNGQLQVFQWGLPNEGPFFFFQIDVDGDGKQDAM
                     VQRNVGGKRTFFIRRSSDGQMYYISWGTASNLFFPLFGDYDGDGKTDFVSRETLDAGT
                     GKFRWHIFQSATQTSRHVDFGQSGDALHAGEPQDELPVYSEQFFK"
BASE COUNT         1251 a         1386 c         1281 g         1483 t
ORIGIN      
        1 agtcttcatt aggaaaggag aagcgcctgc ttcatccttt tttcaaacgc acaaatcaag
       61 tccgaaatac ttacttctcc gttgaaaata gtttttcgat tgttgctttg agcgcgtttc
      121 cgtgcagatt tttggcgatg acttttcccg aattatcaac caaaacattt gccggcaagg
      181 atgtgaccgc gtaatcgaaa gtaaccggcg tttcaaattc ctgaaaggtt gaaacattcg
      241 tccaaattct tttatcctgc tcgatggctt ttagccagtt ttctttttca gactccagac
      301 cgacgccgta aatatcgaaa ccttccggct taaatttttg ataaagttcg ccgagcagac
      361 ggctttcccg acggcacggc aggcaccagc ttccccagaa atcaatcaga atgtattttg
      421 cattgattga agacagcgac acgatttcgc cgtttttatc gggcattttg atttccgcga
      481 ctcgtccgcc gagccggttg gcggttatcg cttttacctt ttcattgatt ctggcggcaa
      541 tctccgtatt tgggtgattg ctttcaaatt gtccggctaa ttcaatcaaa aaaggaatgt
      601 ttttttctcc gtcccagcga atcgaagtcg gataaactgc tatggaagtt cccatttctt
      661 ttttaataaa ttcaatcaat tcgtctttgt gccggtcgta attttcgatt tcgagcccgg
      721 caagctcgag caatttcgaa tcgttttcgg gcgcgccttt ttccttcaat tccttgattt
      781 gattgcggat agaaatgacc agccggttca gcgactcttt tctgaatttt tcgtaagcct
      841 caagttttgc ggtatcttcc gagcctgtta cctgcaaccg acccgaatcg gcggcatcgc
      901 ccgtgataat gatacgctga cctttttcta cggcgagcat aaccgacttt ttatcgttta
      961 tttttaaact gtaaatgtgc ggcggcaatt ctttttcaaa cttaaaacgc ccgtctccgt
     1021 caatcggaat ttcagcgata attttgcttt ttttgcgatt gacgtcttct tccaaaatca
     1081 gttgcagcgt tcccgcaggc agacttttta tttcgcccga tatgctgttt tttgccggtg
     1141 tttttataga acaaccgata ccgagcgaaa aaaagagcaa catcgggtaa aggaaggatt
     1201 tagtttttgc cattattttt tttggtaata atatagttca atcatctatt tttcgttttt
     1261 ccgttcatat gttcggaaag cgcgtcggag actcgtttta ctctctgata gcggctatcg
     1321 tttgagcgcc acgcaattaa aacgtccggc gtaattcccg ccacttcgtt tgtgccgtcg
     1381 gctcgaagcc gcacgcaatc cggtattttt actccggcgc ggctgttttt cagatatgtt
     1441 tgtatcccgc cgtttgtata accgcagccc gcgccgccgg tcggctcgcc gataatggtt
     1501 gccgcattat tgtcttgcag catcgccgcg aaatattccg ccgacgacca cgttccctga
     1561 tcgaccagaa ccatcaatcg tcccgcatat acgccctcgc gatacgcgta acggctcgga
     1621 tagaaaagga aacggctcga cggtttatcc ggcagcgaac ccggctcggc aaaagaaaga
     1681 acgctctgcg ggtatagcgg cggcgcagtc acgacaagtg aacagtccgg ttttcgattt
     1741 tcccagacgc cgccgcggtc acacggctgt tgagattccg atatcgcctt tcgtagaata
     1801 ttttcgccct gtagtaaaag ctctcgattc ggcgacgccg attggcgggc atcggcttca
     1861 attatttcca gacgcctttt cagttgtttc gtccaatgct cgtgccggat aaactgctgg
     1921 cgcggcgagc gaagcggttt atccgaaagc gttcgagcag ccggctccgt ccaattcgtc
     1981 ccgccgccgt ttccggtgat gtctgccagc aaggcattta tcctattgcg cttaagggct
     2041 tcaatctgcc gggcaagcgc cgccgtcagc agatttgccg cccttcgttc cactttatct
     2101 tcacattcgg catcgcacgg cgaatctttc gttaaactca tttgggcggc tgcctgctcg
     2161 cataaatcgg gaaacgcata ttcgctaaag atagcaattc tgatgacgcc gacatcgctt
     2221 tgggcggctg cgcgcaaaac tccgatggag aaatatttcg agtcttcagt attgatttcc
     2281 cgaaaattgt ccagcctggt aaaagcgatt ctgggcgtct ggttctgact ctggtagccg
     2341 agccgccggc ataaaggcat ttggctgttt ggctgcgcat tgtcggcggg cgaagaatta
     2401 gtaaccttat tctgcgccca ttgaacgctt aaatgcccgt ctccgaaagc atccagaaac
     2461 gattcgatta tttttttcgc ttccggttca ctgcgcgcgc cgagcaggcg agattcggtt
     2521 tgcccggaca actgcttgag atcaagtccg cgattctcga cagcccattc aagattggcg
     2581 tacgaggcgg acatctcccg cttcagttga gcgaagtctt ccgaccagac atccgtctta
     2641 aaatcctgac cacgggcagc gccgcccgcc gctaacacca gcaatacgaa aacaagggtc
     2701 aatgtcgaat tccgattcat aattcaaaaa tcatcgtagc gtttcgtctt aaaagggact
     2761 tgtttaactg ttccggtaaa acggagctcg gctgaaatcc ttcaaacgaa ttattaattg
     2821 cgggttcgtt aataaacagc tcccgaatta attgatgtcc ttgcctgctt tcaacagttc
     2881 aatcatcgcc tgaacagctt gtttgccggc atcattcggc gctaaaggca gagccttgct
     2941 ggcgaattcc agggctttcg cgtattcgcc attggcggac aatcctctcg ccatgcccgt
     3001 caacgttata aactgattcg gatttttgtc gtaattaaac tgaaaaacct ccagtgccgc
     3061 ctgcggcttt ttcgcattta aaagctgtct gccgaattgc tgaagctgga cgacgccgcc
     3121 gaaaggcaaa gcgcttttga tgaccgccgc cgcctcgtcg gttctgccga gcttatcgag
     3181 aatcatcgct ttagtcgaaa gcgcgggaaa cgaagtcggg tcgcccggaa atttggagcc
     3241 cgtcgccgcc gttgcccatt cgagtccttt ttccagctgg taattattct gcaaagtcca
     3301 gctcgcggcg atgtttaacg actgcgacgt aaagctgcgc ggattttgcg attcggcgac
     3361 gaaagcatcg aattgctgtt tcaaataatc gccttcgacc ttgaaaggca tcgacagttt
     3421 ttcccacgct aaggtgatga ccgccgagtt cggcgtcgaa tccgaaaact cgtatttcag
     3481 aaactcgacg cttttctcga tcggctgagg tttgattttg acgcgcagag cgtcttcttt
     3541 ttcgtcgtaa aaaaatgaac cccagccgtc cgaccttttg gaaaaaataa cggtgctctc
     3601 cgccgggtca tacgcgataa ataaaccgta ttttcctttc ggcagagttt gaccttcgat
     3661 ttttacgtcg ttatcgaatt cgatgacggt attttcgttc gcgccggcgc gccacggcgc
     3721 gggcttgccg tttccgaatc cctgatcgac aaagcctttg tgaacgattc cgccccaaat
     3781 tttcccttcg cgtcccttga ctgcgggacg atgataagta atggtgacct gcgtcaaacc
     3841 gacttgctcg gagaccgccg cttttttcgt gtagccgttc ggaaccgccg agttatacgg
     3901 cacttgcgcg tacagcggaa cggcaaacaa aacgattgcc agtaaaaata aataattttt
     3961 catatttttg aattgagagc gtaaacgaac cgctaaataa ttgaattttt ttatgcctga
     4021 tgtaaaccga atcaaaaatt atttgaagct ccaactatat caaagattat tcgtatcccg
     4081 attgaatttc agatgctctt tttaataaaa gaaggtaatg aaccctgctc gctacgaatt
     4141 cattaccttc tgtcaaggat tcggctttag aaaacaaatc taaagccgaa tctctgatta
     4201 ggaagcgccc ggaagcccgt ttgttgaaat taaacgactc cggattttgg aattcagcga
     4261 tttatttaaa aaactgctcg ctgtaaacag gcagctcgtc ttgcggttct cctgcgtgca
     4321 acgcgtcgcc ggattgcccg aaatcaacgt ggcgtgacgt ttgcgtcgcg ctttggaaga
     4381 tgtgccagcg aaactttccg gttcctgcgt cgagtgtttc gcgggagaca aaatcggttt
     4441 tgccgtcgcc gtcataatcg ccgaacagag gaaagaagag gttcgaggca gttccccacg
     4501 atatataata catctgtccg tcggaacttc ggcggataaa aaacgtgcgt tttccgccca
     4561 cgttgcgttg taccatcgcg tcttgtttac cgtcgccgtc aacgtcaatt tggaagaaaa
     4621 agaatggacc ctcgttaggt aagccccact gaaacacctg caattgtcca ttagagctgt
     4681 tgcgtatata ccagtaacgc aatccgttct caacgcgagt aacgcataaa tcagctttgc
     4741 cgtcgccgtc gtaatcaccg actgccggtg catcattggt cgttccccaa tattcgtagc
     4801 ggggattgcc ggtcgagctt tcgatgatat accagacgcc ggtggcgcgg cgcataacgg
     4861 caacgtccag cttgccgtcg ccgtcataat cggcggggac gaatgcatct gagccggtag
     4921 gggcaaactg tccccaatct aagacgcgta ttgaatttgt ttcgctttgc aggataaacc
     4981 acctgatgac gttgttatca tcgagattcc acgctacgcg gtcggctctg ccgtcgccgt
     5041 cgaaatcgcc cagattattg acaatccccg gcaagtcgtc gagtccccta ccgacctcaa
     5101 tagaagtagt gagattgttt tctaaactgt ttaaagtata gaacgtgaag tttgattggc
     5161 gaaaaacgac gaggtccgag cgcccgtcgc cgtcataatc ggcgggaata ttggctcgtt
     5221 tgacctgccc acgaatctcg ccgttgggga aattcgcggt gtgaatgttg acgtaccatt
     5281 tatttgagcg cagctcggcg acttgcgcgg gcgtcgtggt aaaggtcagc ggaccgatgg
     5341 ttccggtcgt gccgcctgtg taattaaaat taaaacgaat cggaccgctg acgccgaccg
     5401 g
//
LOCUS       DASWNE010000131         3822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_251989, whole genome shotgun sequence.
ACCESSION   DASWNE010000131 DASWNE010000000
VERSION     DASWNE010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251989"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1257)
                     /locus_tag="VGB68_02750"
     CDS             complement(<1..1257)
                     /locus_tag="VGB68_02750"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEY0048177.1"
                     /translation="MKRIQGAALQSDYAYRQTAYLSNNIGARLTGSPQAERAVQYVAE
                     EMRRIGLDVRLQKLTVPHWVRGEERAELVEFQGMAQGTTQKLVVTALGGTIATAPEGL
                     VGEIVVVDSFDELNQLGRERVQGKIVLFNYRFDRELQASGFGGAAYGQATQYRGGAAI
                     AAGRLGAIGVLVRSAGGSQNRLVHTGGMRYDPNVTKIPAAATSFEDAEMIAYLARMGK
                     VRVKMVLTPQTLPDTTSYNVIADLKGSEKPDEVVIVSGHLDSWDLGTGALDDATGVAV
                     SMQVPFLLKQLKIRPKRTIRVIAYMNEENGLVGGTTYAREEAANAAKHFAAIESDLGA
                     SRPLGFYFTGKPEIAAYLAPISNVLREQGAGLSQLQPGGVGADIGPLTQKGVPSFAPW
                     FDTRTYFNYHHTAADTFDKVNPKELAE"
     gene            1662..3701
                     /locus_tag="VGB68_02755"
     CDS             1662..3701
                     /locus_tag="VGB68_02755"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY0048178.1"
                     /translation="MKRLLLVFFVFAFVSINAQAQTAFTFNEMIRMRRVGDAQLSPDG
                     RTVAFTIGDVNAAENRTLTQIYTMPVGGGNFRQITKGDRSNSSPRWSPDGKKIAFTTG
                     GQIWIMDADGDDRRQITKISTGAGAPVWSPDGRWIAFVSEVYPDCSSDECNQRRDAEA
                     ESSKVQAKVTDRLLYRHWTEWRNTKRSHVFVVSADGGVARDLTPGDFDAPPYGASTGV
                     DYAFSPDSGEIAFLKNPDPVEAISTNSDVFIVPIRGGTARNITVNNKGYDVAPLYTPD
                     GRYILFRSQKTEAFEADRWRIMRYDRKTGATVELTAGFDLQADEMILAPDGKTIYFTA
                     NSRGMQPIYRVPLEPDFRQKIATHVRLISGAGFFSSLNITPDGKTIVAISNSMNHPNE
                     VYTINADTNAVTNLSRVNAEMTALKKPEEIEWKGALNQNIHGFIVKPANFDANKKYPL
                     MVLIHGGPQGAWNNNWSWRWNPQLFANAGYIVFLPNPRGSTGYGQKFVNEISGDWGGK
                     AYTDIVNGVAEVLKRNPYIDKNRIGAAGASYGGYMVNWILGHNNDPRFRFKALLSHAG
                     VYNLESMAGATEELWFVNYEFKGMPWENPQMYARWSPSRFVKNFNTPTLVTHGELDYR
                     VPIGEGLQLYTALQRRGVESKLVYFPDEGHWILKPQNSQFWYKQVFDWFDKHLNP"
BASE COUNT          894 a         1064 c         1005 g          859 t
ORIGIN      
        1 ttcggctaat tccttcggat taactttgtc gaaagtatcc gccgcggtgt gatgataatt
       61 aaaataagtg cgcgtatcga accacggcgc aaacgacggc acgcctttct gcgtcaaagg
      121 tccgatgtcc gcgccgacgc cgcccggctg aagttgcgaa agtcccgcgc cctgctcgcg
      181 cagaacgttt gaaatcggcg ctaaataagc ggcgatttcc ggttttccgg taaagtaaaa
      241 tccgagcggg cgcgacgcgc ccaaatccga ttcgatggcg gcgaaatgtt tcgccgcatt
      301 tgcggcttct tcccgggcgt aagtcgtgcc gccgaccaag ccgttttcct cgttcatata
      361 cgcgataacg cgaatcgttc gtttcgggcg gattttaagc tgtttcaaca aaaacggcac
      421 ttgcatcgaa accgccacgc cggtcgcgtc gtcgagcgcg cccgttccca aatcccacga
      481 atccaggtga ccggagacga tgacgacttc gtcgggcttt tcgcttccct tcaaatcggc
      541 gatgacgttg tagctcgtcg tgtcgggcag agtttgcggc gtcagaacca ttttcacccg
      601 gactttcccc attctggcga gataggcaat catttccgcg tcttcaaacg aggttgccgc
      661 cgccggaatt ttcgtcacat tcgggtcgta gcgcattccg cccgtgtgaa cgagccggtt
      721 ttgcgagccg cccgccgagc gaaccagaac gccgatcgcg cccagacgtc ccgccgcaat
      781 cgccgcgccg ccgcgatact gcgtcgcctg cccgtaagcc gcgccgccga aaccggacgc
      841 ctgcaattcg cggtcgaatc tgtaattgaa cagaacgatt tttccctgca cgcgctcgcg
      901 tccgagttga ttcaattcgt caaagctgtc gaccacgacg atttccccga ctaagccttc
      961 gggcgcggtg gcgatggttc cgccgagcgc cgtgacgacg agtttttgag tcgtgccctg
     1021 cgccattccc tgaaattcga cgagctcggc gcgttcttcg ccgcgaaccc aatgcggcac
     1081 ggttaatttc tgcaaacgga cgtccagacc gatgcggcgc atttcttcgg cgacgtattg
     1141 cacggcgcgt tcggcttgcg gcgagccggt caggcgagcg ccgatattat tggaaagata
     1201 cgccgtttgc cggtaagcgt aatcgctctg caaagccgcg ccctgtattc ttttcaaatc
     1261 ttttaaggtt tgttccgaat acaatcgcgg cgtcggcgtc gggctgggct gcgagaaggc
     1321 tgaaaccgaa aaaattaata ttgataagag aagatataga aaattccgtt tcataaattt
     1381 atttgtttta atgataaaca actcgaacgg caaagtctaa atacgacgta aataccaaag
     1441 gcaaaatgaa aaggagagct tcatttattc gccgacatta attgaatgcg aaattatcgt
     1501 ttttagactt gtaaatgccg tttgcaagct ggcaaacagc cttcgggagc tttcaaatat
     1561 ccatttccat tttgcgcttt gcattaatag acgttttgaa tttgggaaat ttctgccata
     1621 atattggcgt ttttacatct tcaaattttt cggatgaaac tatgaaacgc ttattgcttg
     1681 ttttctttgt tttcgcgttt gtttcgataa acgctcaggc gcagaccgct tttaccttta
     1741 acgagatgat tcggatgcgg cgcgtcggcg atgcgcagct ttcgcccgac ggtcggacgg
     1801 tcgcctttac catcggcgac gtcaacgcgg cggaaaatcg cactttgacg cagatttaca
     1861 cgatgccggt cggcggcgga aatttccgcc agattaccaa aggcgaccgc tcgaacagct
     1921 cgccgcgctg gtcgcccgac ggcaaaaaaa tcgcgtttac gaccggcggg caaatctgga
     1981 ttatggatgc cgacggcgac gaccgcagac aaatcaccaa aatttcaacc ggagcaggcg
     2041 cgccggtctg gtcgcccgac gggcgctgga ttgcttttgt ctccgaagtt tatcccgatt
     2101 gttcgagcga cgaatgcaac cagcgccgcg acgccgaagc cgaaagcagc aaagtgcagg
     2161 cgaaagtcac cgaccgcctg ctttacaggc actggacgga atggcgcaac acgaaacgct
     2221 cgcacgtttt tgtcgtttcc gccgacggcg gcgtcgcccg cgatttgacg cccggcgatt
     2281 tcgatgctcc gccttacggc gcttcgaccg gcgtcgatta cgctttttcg cccgattccg
     2341 gcgaaattgc ttttttgaaa aatcccgacc cggtggaagc gatttcgacc aacagcgacg
     2401 tttttatcgt tccgatacgc ggcggcaccg cgcgcaatat taccgtcaac aacaagggct
     2461 acgacgtcgc gccgctttac actcccgacg gcagatatat tctgtttcgc tcgcaaaaga
     2521 cggaagcctt tgaagccgac cgctggcgca ttatgcgtta cgaccggaaa acgggcgcga
     2581 ccgtcgaatt gaccgccggc ttcgatttac aggcagacga gatgattctc gcgcccgacg
     2641 gcaaaacgat ttatttcacg gcgaactcac gcggaatgca gccgatttac cgggttccgc
     2701 tcgaaccgga ctttcggcaa aaaatcgcca cgcacgtccg gctcatctcc ggcgcgggct
     2761 ttttcagcag cctgaacatt acgcccgacg gcaaaacaat cgtggcgatt tccaattcga
     2821 tgaatcatcc gaacgaagtt tacacaatca acgcggacac gaacgcggtc acgaatctga
     2881 gccgcgtcaa cgccgagatg acggctttga aaaagcccga agaaatcgaa tggaaaggcg
     2941 ctctgaatca aaacattcac ggttttatcg tcaaaccggc gaatttcgac gccaataaaa
     3001 aatatccgtt gatggttctg atacacggcg gaccgcaggg cgcgtggaac aataactgga
     3061 gctggcgctg gaacccgcag cttttcgcca acgccggtta catcgttttt ctgccgaacc
     3121 cgcgcggctc gaccggttac gggcagaaat tcgtcaacga aatttccggc gattggggcg
     3181 gcaaagccta cacggacatt gtgaacggcg tcgcggaagt tttaaaacgc aatccgtata
     3241 tcgacaaaaa ccgcatcggc gcggcgggcg ccagctacgg cggttatatg gtcaactgga
     3301 ttctcggaca caacaacgac ccgcgctttc gcttcaaggc gctgctctcg cacgccggag
     3361 tttacaatct ggaaagtatg gcgggcgcga ccgaagagct ctggttcgtc aattacgaat
     3421 ttaaaggaat gccctgggaa aacccgcaga tgtatgcgcg ctggtcgccg agtcgattcg
     3481 tcaaaaattt caacacgccg acgctcgtca cgcacgggga gctcgattac cgcgtgccga
     3541 tcggcgaagg attgcagctt tacacggctt tgcagcggcg cggcgtcgaa tcgaagctcg
     3601 tctattttcc cgacgaagga cactggattc tgaaaccgca aaattctcag ttctggtaca
     3661 aacaggtttt cgactggttc gacaaacatc tgaatccgta aggaaattag cggtaaacgg
     3721 taagtggtaa gtggtaagtg aaaagccgaa acttaccact taccacttac cgtttaccac
     3781 ttaccgctta ccacttttaa agggtttatg aaatttcgaa aa
//
LOCUS       DASWNE010000132         1522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_4461, whole genome shotgun sequence.
ACCESSION   DASWNE010000132 DASWNE010000000
VERSION     DASWNE010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4461"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1522)
                     /gene="acs"
                     /locus_tag="VGB68_02760"
     CDS             complement(<1..>1522)
                     /gene="acs"
                     /locus_tag="VGB68_02760"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEY0048179.1"
                     /translation="IKSFEEYEQIYNKAAEDPEAFWASVAENLHWFKKWDKVLEWDEP
                     HAKWFVGGRINASYNCLDRHLDGWRKNKAAVIWEGEPGEIRTLTYQQMHREVSRFANV
                     LKKTGIKTGDRVALYMPLVPELAIAMLACARIGAPHTVIFGGFSADAIRDRVNDGECK
                     LIVTADGGYRRGSEIRLKETVDAAVAQTPTVENVIVFQRTGSKISMKHGRDHWWHELM
                     ETVSDDCPAEELDSEHPLYILYTSGTTGKPKGILHTTGGYLTGTYITSKWIFDLKDED
                     TYWCTADIGWVTGHSYVVYGPLANGATVFMYEGAPNYPEPDRFWRIIERHKINIFYTA
                     PTAIRAFIKWGEQHPLKHDLSSLRLLGTVGEPINPEAWMWYHTIIGKTKCPIVDTWWQ
                     TETGAIVISPLPGATPTVPGTATRPFPGILIDIQTRDGKSVGTNEGGYLVITHPFPSM
                     LRTIWGDDERFKKQYWSEIPGVYFAGDGARRDRRGYYWIMGRVDDVINVSGHRLGTA"
BASE COUNT          308 a          436 c          411 g          367 t
ORIGIN      
        1 cgccgtgccg aggcgatgac cggaaacgtt tatcacgtcg tcgacgcgtc ccataatcca
       61 gtaatagccg cgccggtcgc gccgcgcgcc gtcgcccgcg aaataaacgc ccggaatttc
      121 cgaccagtat tgttttttaa atctttcgtc gtcgccccaa atcgtgcgca gcattgaggg
      181 aaacgggtgt gttatgacga gataaccgcc ttcgttcgtg cctaccgatt ttccgtctct
      241 cgtctgaata tcaatcaaaa tgcccggaaa aggacgcgtc gccgtgccgg gaacggtcgg
      301 cgtcgcgccc gggagcggcg aaatcacaat cgcgccggtt tccgtctgcc accacgtatc
      361 gacaatcggg catttcgttt tgccgataat cgtgtgatac cacatccacg cttcgggatt
      421 tatcggctcg ccgacggttc cgagcagccg caaggaagat aaatcgtgtt tcaaaggatg
      481 ctgctcgccc catttgataa aggcgcgaat cgcggtcggc gcggtgtaga aaatattgat
      541 tttgtggcgt tcgatgatgc gccagaagcg gtcgggttcg ggataattcg gcgcgccttc
      601 atacataaag accgtcgcgc cgtttgccag cggtccgtag acgacgtaag aatgtccggt
      661 cacccagccg atgtccgccg tgcaccagta agtatcttcg tcttttaaat cgaaaatcca
      721 tttgctcgtg atataggttc ccgtcaaata accgccggtc gtgtgcagaa ttcctttcgg
      781 ctttccggtc gtgccggaag tgtaaagaat ataaagcggg tgctcgctgt cgagttcttc
      841 ggcgggacaa tcatcgctga cggtttccat cagctcgtgc caccagtggt cgcgtccgtg
      901 cttcatcgaa attttcgagc cggttctttg aaaaacgata acgttttcga ccgtaggcgt
      961 ctgcgcgacc gccgcatcga ccgtttcctt caacctgatt tcgctgccgc gccggtagcc
     1021 tccgtcagcc gtgacgatta atttacattc gccgtcgttg acgcggtcgc gaatcgcgtc
     1081 cgccgaaaat ccgccgaaaa ttaccgtgtg cggcgcgccg atgcgggcgc aggcgagcat
     1141 cgcaatcgcc agctccggca cgagcggcat ataaagcgcc acgcggtcgc cggttttgat
     1201 gccggttttt ttcaggacat tggcgaatct ggaaacctct cgatgcatct gctggtaagt
     1261 tagagtgcgg atttcgcccg gctcgccttc ccagataacg gcggctttat ttttgcgcca
     1321 gccgtccaga tggcggtcga ggcaattgta ggaagcgtta atgcgcccgc cgacaaacca
     1381 tttcgcgtgc ggctcgtccc actccagaac cttgtcccat tttttaaacc agtgcaggtt
     1441 ttccgccacc gaagcccaga aagcttccgg gtcttcggca gctttgttat aaatctgctc
     1501 gtattcttca aagcttttga ta
//
LOCUS       DASWNE010000133         4097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_215570, whole genome shotgun sequence.
ACCESSION   DASWNE010000133 DASWNE010000000
VERSION     DASWNE010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215570"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VGB68_02765"
     CDS             complement(<1..311)
                     /locus_tag="VGB68_02765"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEY0048180.1"
                     /translation="MLTKELEETLGIAVDEAVKRRHEYVTLEHLLYALLEDKTARDVL
                     VNCGVDLEKLAASLDEYFSNALEILPDKVQLMPELTSTFQGIIQYAMMQAEGSGQRVV
                     DG"
     gene            complement(316..789)
                     /locus_tag="VGB68_02770"
     CDS             complement(316..789)
                     /locus_tag="VGB68_02770"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEY0048181.1"
                     /translation="MKIVEIKNLDGEVIFSYDEEDEKWIWGDYDFSGVNLEKANLEGI
                     VWQQISLRRANLKEADCYRAILCGSDLSESDCESAEFTGANLKQVNFTKASLKNAVFT
                     KDNLGGSTDVSGANFNGAVLDGAEFKETFYNARTIFPENFNPEKNGLVRIKTPIE"
     gene            complement(795..1106)
                     /locus_tag="VGB68_02775"
     CDS             complement(795..1106)
                     /locus_tag="VGB68_02775"
                     /inference="COORDINATES: protein motif:HMM:NF014655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HEY0048182.1"
                     /translation="MPNVPDFDADVLTESETKLEKPPLYKVVLHNDDFTTMDFVVYVL
                     QTVFHRSDMEAFAIMFKVHTEGVGVAGVYTYEIATMKAEKATNLARAREYPLLCTVEE
                     E"
     gene            complement(1250..2362)
                     /locus_tag="VGB68_02780"
     CDS             complement(1250..2362)
                     /locus_tag="VGB68_02780"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY0048183.1"
                     /translation="MKAIAVTPKQKDSARLVEMDAPNIADVPNGRGVLVEVLRVGACG
                     TDREINNAEYGVAPEGFDFLVLGHENFGRVVETGENVKELSKGDYVVATVRRPGKSFY
                     DRIGEQDFTTDDEYFERGISRLHGFMAEFYAESADFLIKIPPAIREAGVLLEPLSIIE
                     KGLKQASDIQERLKIWRPKTAAVLGTGSVGLLTVMALRLRGYEVHGFGKQTRAGYLNA
                     ELCQSIGATYDSTDEISVVDSVKKYGEYDLIFECTGFSPIVFDAMQSLNQNGVLILSS
                     VTGGDRVAEVPSDKINQSFVLGNRAMFGTVNANREHFELGVKDLALAEAMYAGWLGRM
                     LTHPVKGLENYAQVFDILNDAGAHKAIKTYFEVGEI"
     gene            complement(2386..4071)
                     /locus_tag="VGB68_02785"
     CDS             complement(2386..4071)
                     /locus_tag="VGB68_02785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0048184.1"
                     /translation="MKRSIFGFAVFVLILVFSAQARAQGRCFTEEEAKKIVDSINAPQ
                     KTPENKKLREELLKMQATRGELNLKAVENGEKNPKLIAEANQSGENQLLRLCQIIKEN
                     GWLSNEMVGEDAVAILLSIIRNNKAFDLQKEFFPVLSAAAEKGLVSKANLAWLIDSVR
                     LGGGQPQVFGTQSHVKNEIIYLYPILNEEKLDKWRALYDLPPLAEFIKDLQVRYQTVV
                     IKAPRLPAPPVLKQPASKNSEPSAPGLEEEENEVVKVETQLVNMNVRVLNEDLSSAGN
                     LNLKKEDFAVFEEGQEQEIAFFSTTDKPFDLILLLDLSVSLKGKQDLIIESAQRFVQA
                     ARPTDRIAVVTFTHEAKTIADLTSDKKVLLEKIKGINAAKGGSKVWDALKFTYDGIVK
                     TESAGRRSAIVFMTDGVDNSLYQDIRRWERRNSPDPVTGSIIGLNPLNAPSETTFTEL
                     LEIVRQNEATIFPIYLDSEALNTDWYKKAVRLGRRSLEMLAEETGGQNYYAKKINDLN
                     GIYEKIIDDLSRVYSLGYESGNETRGGAWRSLSVKIKNQPKLIVRTKRGYYAK"
BASE COUNT          914 a         1126 c          938 g         1119 t
ORIGIN      
        1 ccgtcgacga cgcgctgccc gctgccttcg gcttgcatca tcgcgtattg aatgatgccc
       61 tgaaaggtcg aggtcagctc cggcatcagc tgcaccttat ccggcaaaat ttccagagcg
      121 ttgctgaaat attcgtccag actcgccgcc agcttttcca aatccacgcc gcaattgacc
      181 agcacgtcgc gcgccgtttt atcttccagc agcgcgtaaa gcaaatgctc cagcgtcacg
      241 tattcgtggc gtcttttcac ggcttcgtca accgcgattc ccagggtttc ttctaattct
      301 ttcgttaaca taaatctatt ctatcggtgt tttgatgcga accaagccgt ttttttccgg
      361 gttaaaattt tccggaaaaa tcgtccgcgc attgtaaaac gtctctttaa attctgcgcc
      421 gtccaaaacc gcgccgttaa aatttgcgcc gctcacatcg gtcgagccgc caagattgtc
      481 ttttgtgaag acggcatttt tcagactcgc tttggtgaaa ttcacttgct ttaaattagc
      541 gcctgtaaat tccgcgcttt cacaatccga ttcgcttaaa tccgagccgc acagtatcgc
      601 ccgatagcag tcagcttctt ttaaatttgc gcgccggaga gaaatttgct gccaaacgat
      661 tccttccaga ttcgcttttt ccagattgac gccggaaaaa tcgtaatcgc cccaaatcca
      721 tttttcatct tcttcgtcgt aactgaaaat cacttctccg tcgagatttt taatttctac
      781 gattttcata aagcttattc ttcctcgacc gtgcacagca gcggatattc gcgcgcgcgc
      841 gccagattcg tcgctttttc ggctttcatc gtggcgattt cgtatgtgta gacgcccgcc
      901 acgccgacgc cttccgtatg aaccttgaac ataatggcga aggcttccat atccgagcga
      961 tgaaaaaccg tctgtaagac ataaacgaca aaatccatcg tcgtaaaatc gtcgttgtgc
     1021 agaacgacct tgtaaagcgg aggcttttcc agcttcgtct cgctctcggt taaaacatcg
     1081 gcgtcaaaat cgggaacatt cggcataaga taattttgcc acagactcga cggagaaaac
     1141 aggggaaaat ttaacaggat agacaggata cgcaggataa tttgaagcta tgaacattaa
     1201 aagttctttt ttatcctgta aatcctgttc atcctgtttg aatctgccgt taaatttctc
     1261 cgacctcaaa ataagtctta atcgctttat gcgcgcccgc gtcatttaag atgtcgaaaa
     1321 cctgcgcgta gttttccaag cctttgaccg gatgcgtgag cattcgcccc agccagcccg
     1381 catacatcgc ttccgccaga gccaaatcct tgacgcccag ctcgaaatgc tcgcgattgg
     1441 cattgaccgt gccgaacatc gcgcgatttc ccagcacaaa gctctgattg attttgtcgg
     1501 acggcacttc cgccacgcgg tcgccgcccg tgaccgaaga caaaatcaaa acgccgtttt
     1561 gattcaggct ctgcatcgcg tcaaagacga tgggcgaaaa tccggtgcat tcaaaaatca
     1621 aatcgtattc gccgtatttt ttcacggaat cgacgacgga aatttcgtcg gtcgaatcat
     1681 aagtcgcgcc tatgctctgg caaagctcgg cgttcagata accggcgcgc gtttgtttcc
     1741 cgaagccgtg aacctcataa ccgcgcaggc gcaaagccat cacggtcaaa agcccgacgc
     1801 tgcccgtgcc cagaacggcg gcggttttcg gtcgccagat tttcaggcgt tcctgaatgt
     1861 cggaagcctg tttcaagccc ttttcgataa tcgaaagcgg ctctaaaaga acgcccgctt
     1921 cgcgaatggc gggcggaatt ttgatgagaa aatccgcgct ttcggcgtaa aattccgcca
     1981 taaacccgtg cagccgcgag atgccgcgct cgaaatattc gtcgtcggtc gtaaaatcct
     2041 gctcgccgat ccggtcgtaa aagctttttc ccggacgcct aacggtggcg acgacgtaat
     2101 cgcctttgct caattctttg acgttttcgc ccgtttcgac gacgcgcccg aaattttcgt
     2161 gaccgagcac gagaaaatcg aagccttcgg gcgcgacgcc gtattcggca ttattgattt
     2221 cgcggtcggt tccgcacgcg ccgacgcgca aaacttcgac cagaacgcct cgcccgtttg
     2281 gaacgtcggc gatattcggc gcgtccattt cgaccagacg cgcgctgtcc ttttgtttcg
     2341 gcgtcacggc tattgctttc atagcttata gtttaccttt tatctctatt ttgcgtaata
     2401 accgcgtttg gtgcggacga ttaatttcgg ctggttttta attttcacgc tcaggctgcg
     2461 ccacgcgccg ccgcgcgtct cgtttcccga ttcgtatccg aggctgtaga cgcggctcaa
     2521 atcgtcgatt attttttcgt aaatcccgtt taaatcattg atttttttgg cgtaataatt
     2581 ttgcccgccg gtttcttcgg cgagcatttc caggctccgg cgtcccagcc tgaccgcttt
     2641 tttataccaa tcggtgttca gagcttcgct gtccagataa atcggaaaaa tggtcgcttc
     2701 attttgcctg acgatttcca aaagctcggt aaaagtcgtc tccgaaggcg cgttcagcgg
     2761 atttagaccg ataatgctgc ccgtcaccgg gtcggggctg tttctccgct cccacctgcg
     2821 tatgtcctga taaagcgaat tatcgacgcc gtcggtcata aagacgatgg cgctgcggcg
     2881 accggcgctc tcggttttca ctatgccgtc gtaagtaaat ttcagcgcgt cccagacctt
     2941 cgagccgcct ttggcggcgt taattccttt aatcttttcc agaagaactt ttttatccga
     3001 cgtcaaatcc gcgattgttt tcgcctcgtg cgtaaaagtg acgaccgcga tgcggtctgt
     3061 cgggcgcgcc gcctgcacga aacgctgcgc cgattcgatt atcaaatcct gttttccttt
     3121 gagcgagacg gaaaggtcga gcagtaaaat caaatcgaaa ggcttgtcgg tggtcgaaaa
     3181 aaaagcgatt tcctgttcct gtccctcttc aaaaacggca aaatcctctt tctttaaatt
     3241 cagatttccg gcgctcgaca aatcttcgtt caaaacgcga acgttcatat ttaccaattg
     3301 cgtttcgacc ttcacgacct cgttttcttc ctcttccaaa ccgggcgcgg aaggctcgct
     3361 gttttttgac gccggttgtt tcaaaacggg cggcgcgggc aggcgcggcg ctttgatgac
     3421 gaccgtctga tagcgcacct gcaaatcttt gataaattcc gccagcggcg gcaaatcata
     3481 aagcgcgcgc catttgtcga gcttttcttc gtttaaaatc gggtaaagat aaattatctc
     3541 gtttttaacg tggctctgcg ttccgaatac ctgcggctga ccgccgccga ggcgaacgct
     3601 gtcaatcagc cacgctaaat tggctttaga gacgagacct ttttccgccg ccgccgaaag
     3661 aaccggaaaa aattcctttt gcaggtcaaa cgccttgttg tttctgatta tcgataaaag
     3721 aatcgcgacg gcgtcttcgc cgaccatttc attgctcagc cagccgtttt cctttatgat
     3781 ttgacacaat cgaagcagtt gattttcgcc cgattgattg gcttcggcga ttagtttcgg
     3841 atttttctcg ccgttctcga cggctttcag attcagctcg ccgcgcgtcg cctgcatttt
     3901 cagcagctcc tcgcgcagct ttttattttc cggcgttttt tgcggcgcgt tgatggaatc
     3961 gacgattttt ttcgcttctt cttcggtaaa acatcttccc tgcgcacgcg cctgcgccga
     4021 aaaaaccaga atcaggacga atactgcaaa tccgaaaatt gaacgcttca taaaaattta
     4081 tctctcagga atattct
//
LOCUS       DASWNE010000134         5667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_145334, whole genome shotgun sequence.
ACCESSION   DASWNE010000134 DASWNE010000000
VERSION     DASWNE010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5667
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145334"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VGB68_02790"
     CDS             complement(<1..484)
                     /locus_tag="VGB68_02790"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048185.1"
                     /translation="MAQHRALFLTKTEVQRPKTKCEAFMKLIDHSQNLPIRVSTIQII
                     AFILFALLGARLYYLQLVKGEYYAEKAENQRVRYIPIPAPRGAILDRNGTLLVDSRPT
                     YNVVLSHEPIKSINVLERLDDYSRGLKVDRQYLLERLNLIKKQPEFEAMVIKEAATME
                     D"
     gene            complement(527..1081)
                     /gene="mreD"
                     /locus_tag="VGB68_02795"
     CDS             complement(527..1081)
                     /gene="mreD"
                     /locus_tag="VGB68_02795"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEY0048186.1"
                     /translation="MEQVKLTVALIIAILLQWSLRNVAEPFAFVDFPLIVVVYAALQR
                     NSIRALLFGTIAGIAVDALSGGLLGANGFCKTLIAYAVSEIARRVYMDNLLLRIPVIA
                     GACLVNGLLYYGMHRILGQEPTNPVVNTIAYTLIGTTIAGTIVFLILDGLSMEKNRTA
                     RKRDIFTPRRQTRRRNPIRLGRRT"
     gene            complement(1100..2014)
                     /gene="mreC"
                     /locus_tag="VGB68_02800"
     CDS             complement(1100..2014)
                     /gene="mreC"
                     /locus_tag="VGB68_02800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEY0048187.1"
                     /translation="MVERSQKEVWRLTPWLMVALLLGNFVLMAWDAKTDSQQRVLRVW
                     TQAIADFVQSPVTSISSGVSNYFQSFANLRGAASENEQLKQRVQELQVELQGKESFVA
                     ENERLKSLLNLKEESKYPILTAKVIGRDPSGWFNTSIINRGTVDGVKLNMPVVTNGGL
                     VGRVMAVGPLTSQVMLLTDDKSGIAGIVGELGSSNALGVIKGTNEKELVEMRYVPGSI
                     PVQEGETVFTTGQDGIYPAGLKIGEVVRVESGSATTTHTILVKPGGSLNSMQEVAVLL
                     YEAPPPPKFEQTLPNAAKTPGTTNRRQR"
     gene            complement(2108..3157)
                     /locus_tag="VGB68_02805"
     CDS             complement(2108..3157)
                     /locus_tag="VGB68_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY0048188.1"
                     /translation="MALPKSLFSLFSSDLAIDLGTANTLVYAKGRGIVVSEPSIVAIN
                     KVTNQVEAVGRDAKEMLGRTPGNIVAIRPMKDGVIANFEVTEKMLQHFIRKAHNGKSW
                     VRPRVVIGIPSEITQVERRAVEDSAYRAKASEVYLVEEAMAAAIGAGLPITEPHGNMV
                     VDIGGGTTDIAVISLSGIVYSRAVRVAGNEMDESITQFIKRKYNLLIGERTAEAIKIA
                     LGSGFPLEEPLSMEVRGRNLIEGIPKTITITDEEIREALSDAVATIVNAVRVALERTP
                     PELSADIVERGIVLTGGGALLKNLDKRLMIETGLPVVIADDPLSSVVLGTGKMLSDFE
                     LLKRVKWDNSLMTGN"
     gene            complement(3473..>5667)
                     /gene="lon"
                     /locus_tag="VGB68_02810"
     CDS             complement(3473..>5667)
                     /gene="lon"
                     /locus_tag="VGB68_02810"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEY0048189.1"
                     /translation="KRMMRADDTMQLIVQGVERVRVVYWAQEQPFLQAMVEVLPELTI
                     EDAAAVEALKRNVQSLIQQALAMLPNIPPEIRMAVMTANEPVQLAYFLGSVLDLGVDQ
                     EQKMLEANTADELLTLAHASLAREVDIMQIRSKISAEAQTEMDKAQRDYILRQQLKAI
                     QKELGEDETGEKAEAGQLRERLETADLPEDVRKEAERELRRMEQLPQAAPDYHVIRTY
                     LEYILDLPWKKASAEKLDLSEARAILDEDHYGLEDIKERILESLAVVKLRPDSKSPII
                     LFVGPPGVGKTSLGRSIARALGREFDRISLGGVRDEAELRGHRRTYVGAMPGRIIQTF
                     RRVGVNNPVLMLDEIDKMGSDFRGDPASAMLEILDPAQNNSFRDHYLDLPFDLSKTFF
                     IATANQLSPIPIPLRDRMEIIQLAGYSDVEKLNIAKQYLIPRQVKENGLSPEQIEITD
                     DAVNLLTSRYTREAGVRQLERAVGNLARKVALRVAQGSGGDKVTITGQDVKGYLGAPR
                     FYPEEARNELPAGVATGMAWTEMGGEVLFIEATALPGGSGLMLTGQLGDVMKESAQAA
                     RSYLWSHASELGIDAEAIKQNGVHVHVPAGAIPKDGPSAGVTMASALASLYTGRKVRS
                     DTAMTGEITLSGLVFPVGGIKEKVLAAHRAGIRRIVLPERNEADVEDIPEDVRKELEI
                     IPAKRINDVLNAALEKESSYDSAHMPLITSHSNAGNQHDAEHLSMKEE"
BASE COUNT         1258 a         1574 c         1464 g         1371 t
ORIGIN      
        1 tgtcttccat cgtcgccgct tccttgatga ccatcgcctc aaattccggc tgttttttta
       61 tcaggttcag gcgttcgagc aggtattgac ggtcgacttt aagcccgcgc gaataatcgt
      121 cgagccgttc gagcacgttg atggatttta tcggctcgtg cgacagaacg acgttgtatg
      181 tcgggcgcga atcgaccagc aaagtcccgt tacggtcgag aatcgcgccg cgcggcgccg
      241 gaatcggaat gtatcgaact ctctgattct cagccttttc cgcgtaatac tcgcccttga
      301 caagctgcaa ataataaaga cgcgcgccca aaagcgcaaa cagaataaaa gcgattatct
      361 gaatggtgct gacgcggata ggaaggtttt gcgaatggtc gattagtttc ataaatgctt
      421 cgcatttggt tttcggtctt tggacttcgg tcttcgttaa aaataatgct cgatgttgag
      481 ccatagaaaa atcgaagacc gaagaccaaa gaccgaaaac cgatttttaa gttcttcttc
      541 caaggcgaat cggattacgg cgtctcgtct ggcggcgcgg cgtaaagatg tcgcgttttc
      601 tggcggttcg gtttttttcc atcgaaagac cgtctaaaat caggaaaaca atcgttccgg
      661 cgatagtcgt gccgatcagc gtgtaagcaa tcgtattaac gacgggattg gtcggttcct
      721 gccccaaaat gcgatgcatc ccgtagtaaa gcaaaccatt caccagacaa gcacctgcta
      781 tgacgggaat gcggagcaaa aggttgtcca tatagacccg gcgcgcaatc tccgaaactg
      841 cgtatgcaat caacgtttta cagaaaccgt tcgcgccgag cagaccgccg ctcaaagcgt
      901 cgaccgcgat gcccgcaatc gttccgaaaa gcaaagcccg aatcgagttt ctctgcaaag
      961 cggcatagac gacgacgata agaggaaaat ctacaaacgc gaacggctcc gccacgttgc
     1021 gaagcgacca ttgaagcaaa attgcgatga ttagagcaac tgtcagcttt acttgttcca
     1081 tcagttccta aggcttcttt tatctttgtc gtctatttgt tgtaccgggc gttttcgccg
     1141 cgttcggtaa agtttgttcg aatttcggcg gcggcggcgc ttcgtaaagc aaaaccgcaa
     1201 cttcctgcat cgaattcagc gagccgcccg gtttgaccag aatcgtgtgc gttgtcgtcg
     1261 ccgaaccgga ttcgaccctg acgacttcgc cgattttcaa tccagccgga taaattccgt
     1321 cctgtccggt ggtgaaaacc gtttcgcctt cctgcaccgg aatgcttccc ggcacatatc
     1381 gcatctcgac cagttctttt tcgttggtgc ctttaatgac gccgagcgcg tttgaagagc
     1441 cgagctcgcc gacgatgccc gcgatgccgg atttatcgtc cgtcaaaagc atcacctgcg
     1501 aggtcagcgg tccgaccgcc ataactctgc cgaccaagcc gccgttagta acgaccggca
     1561 tattcagctt gacgccgtcc acggttccgc gattgataat tgaagtgttg aaccagccgg
     1621 aagggtcgcg accgatgact ttcgccgtca gaatcggata tttggattct tccttgagat
     1681 ttaaaagcga tttgagacgt tcgttttcgg cgacaaagct ttctttgccc tgaagctcga
     1741 cctgaagttc ctggacgcgc tgtttcagtt gctcgttttc gctggcagcg ccgcgcagat
     1801 tggcaaagga ttgaaaataa ttggaaacgc ccgagctaat gctcgtcacg ggcgattgaa
     1861 cgaaatcggc aatggcttgc gtccagacgc gaagcacgcg ctgctgcgaa tcggtcttcg
     1921 catcccaagc catcagaaca aaattgccca aaagcagggc aaccatcagc cacggcgtga
     1981 gtctccaaac ttctttctga ctgcgctcaa ccatttctta agtttaccga gttgactgag
     2041 ttgaccgaag ttgactgagt ttatgattta actcggcaaa ctcagtcaac tttcagtcaa
     2101 ctcacaacta atttcccgtc atcaatgaat tatcccattt gacgcgcttc aggagttcga
     2161 aatccgaaag cattttgccc gtgccgagaa caaccgagga gagcgggtcg tcggcgatga
     2221 ccaccggcaa gccggtttcg atcatcaggc gtttatcgag attttttaat aaggcgccgc
     2281 cgccggtcaa aacgatgccg cgttcgacaa tgtcggcgga aagctcgggc ggcgttcttt
     2341 ccagagcgac acgaacggcg ttgacaatcg tcgcgaccgc atcggaaagc gcctcgcgga
     2401 tttcctcatc ggtaatcgtg atggttttcg ggatgccttc gatgagattg cgcccgcgaa
     2461 cttccatcga aagcggttct tcgagcggaa agccggagcc gagcgcgatt ttgatggctt
     2521 ccgccgtgcg ctcgccgatt aaaaggttgt atttgcgctt aataaactgc gtgatggatt
     2581 cgtccatctc gtttccggcg acgcgcaccg cgcgcgaata gacgatgccc gagagcgata
     2641 taacggcgat gtcggtcgtg ccgccgccga tgtccaccac catattgccg tgcggctcgg
     2701 taatcggaag ccccgcgccg attgccgccg ccatcgcttc ttcaaccaaa taaacttccg
     2761 aagctttggc gcgatacgcc gagtcttcga cggcgcggcg ctcgacctgc gtgatttccg
     2821 aaggaatccc gatgacgacg cgcgggcgca cccaggattt tccgttgtgc gctttgcgga
     2881 taaagtgctg gagcattttt tccgtgacct cgaaattggc aatcacgccg tctttcatcg
     2941 gacgaattgc gacgatgttg cccggcgtgc gtccgagcat ttctttggcg tcgcgcccga
     3001 cggcttcgac ctgattggtc actttgttga tggcgacgat ggacggctcg ctgacgacga
     3061 taccgcgccc tttggcatag acgagcgtat tagccgtgcc gaggtcaatc gccaaatcgc
     3121 tggaaaataa gctgaataaa gattttggta atgccattta atattcgtgt gttaaattca
     3181 gaattttaca aaacgcggcg agttgggata aatgctccca aaggtttgct catcaaggaa
     3241 atttgagttt tcggctcagg gaaggcaact tctcaacacg cagcactaaa ctatttagaa
     3301 tataccgaat ttccagcgaa ttgctaatag aaaaatgtaa tttttgagat tttttttttg
     3361 aaaagcaaaa ttgcgggaaa atgcggttga aaattaacga atttgtaaag acagtcggcg
     3421 gctgaatatt acctttcaac cgccgactgt cttttgcttt caatcgacga atttattctt
     3481 ccttcatact cagatgttcg gcatcgtgct gatttccggc gttagaatgc gacgttatca
     3541 aaggcatatg cgcgctgtcg taagacgatt ccttttccag cgcggcattg agcacgtcgt
     3601 taatccgttt cgcgggaata atttccagct ctttgcgcac gtcttcggga atatcttcga
     3661 cgtccgcttc gtttctttcc ggcagaacaa ttcgacggat tcccgcgcga tgcgccgcca
     3721 gaactttttc cttgattccg ccgaccggaa aaaccaagcc ggaaagcgta atctcgccgg
     3781 tcatcgccgt atcggaccga acttttctgc ccgtgtaaag cgaagccaga gccgaagcca
     3841 tcgtcacgcc cgcgctcggt ccgtctttcg gaatcgcgcc cgccggaacg tgcacgtgaa
     3901 cgccgttttg tttgattgcc tcggcgtcga tgccgagctc cgaagcgtgc gaccacagat
     3961 aactgcgcgc cgcctgcgcc gattccttca tcacgtcgcc gagctgcccg gtcagcatca
     4021 agccggaacc gccgggcaaa gcggtcgcct cgataaacaa aacctcgccg cccatttccg
     4081 tccaagccat tccggtcgcg acgccggcgg gcaattcgtt gcgcgcttct tcgggataaa
     4141 atcgcggcgc gccgagataa cctttcacgt cctgccccgt aattgttact ttatcgccgc
     4201 cgctgccttg cgcgacgcgc aaagcgactt ttcgagccag attgccgacg gctctttcca
     4261 gctggcgaac tccggcttcg cgcgtgtacc gcgacgtcaa aagatttaca gcgtcgtccg
     4321 tgatttcgat ttgctccggc gacaaaccgt tttctttaac ctgtcgcgga atcagatatt
     4381 gtttggcgat attcaatttc tcgacgtcgg aatagcctgc cagctgaata atctccatcc
     4441 ggtcgcgcaa aggtatcgga atcggcgaaa gctggtttgc cgtggcgatg aagaaagttt
     4501 tcgacaaatc gaacggtaaa tccaggtaat ggtcgcggaa agaattgttc tgcgccgggt
     4561 cgagaatttc cagcatcgcg gacgccgggt cgccgcgaaa atcgctgccc attttgtcga
     4621 tttcgtccag catcaaaacc ggattgttga cgccgacgcg ccgaaaggtt tgaataattc
     4681 tgcccggcat cgcgccgaca tacgtccgcc gatgaccgcg cagctcggct tcgtcgcgga
     4741 cgccgcccag agaaatccgg tcgaattcgc gaccgagcgc acgagcaatc gagcgtccca
     4801 gagaagtttt tccgactccc ggcggtccga cgaaaagaat aatcgggctt tttgaatcgg
     4861 gacgcagctt gacgacggcg agcgattcca gaattctttc cttgatgtcc tccaagccgt
     4921 aatggtcttc gtccagaatc gcgcgcgcct cgctcaaatc cagtttttcg gcgctggctt
     4981 ttttccaggg caaatccaga atgtattcca gataagtgcg gataacgtga taatcgggcg
     5041 cagcttgcgg caattgctcc atccgacgca attcgcgctc ggcttccttg cgcacgtctt
     5101 ccggcaaatc cgccgtctcc aatctttccc gaagttgtcc ggcttcggct ttctcgccgg
     5161 tttcgtcttc gccgagctct ttctgaatcg ctttcagctg ctggcgcaga atgtaatcgc
     5221 gctgcgcttt gtccatttcc gtctgcgctt cggcggaaat cttcgagcga atctgcatta
     5281 tatcgacttc gcgggcgagc gaagcgtgcg ccagcgtcag aagctcgtca gccgtgttcg
     5341 cttcgagcat tttctgttcc tgatcgacgc ccaaatccag caccgagccg agaaaatacg
     5401 cgagctgcac gggctcgttc gccgtcatca cagccatccg tatttccggc ggaatattcg
     5461 gaagcatcgc cagcgcctgt tgaatcaaac tctgcacatt gcgttttaag gcttcgaccg
     5521 cggcggcgtc ttcaatcgtc agctcgggca gaacttcgac catcgcctgc agaaaaggct
     5581 gttcctgcgc ccagtagacg acgcggacgc gctcgacgcc ctgcacgata agctgcatcg
     5641 tatcgtcggc gcgcatcatt cgcttga
//
LOCUS       DASWNE010000135         4163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_215594, whole genome shotgun sequence.
ACCESSION   DASWNE010000135 DASWNE010000000
VERSION     DASWNE010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215594"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VGB68_02815"
     CDS             <1..773
                     /locus_tag="VGB68_02815"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEY0048190.1"
                     /translation="HNREKIIEDYKSGLSYKEIQEKYGVKYKWFHRVLRNNAIELRGH
                     KRKFTQADENSLVEKYQNGSGINALVREYKTSAVTIKNILNKRGIDRKKIGGQPRKWS
                     KEDIEKVIKLYDSGKGLSQQKIAQIMESDQRRISVILRQNGVSTKRISERHPNWKGGR
                     IIDESGYVRVLAKYAGKFAYEMSLSDGYVFEHRAVMAQYLGRPLRKNETVHHIDGNRS
                     NNNIENLQLRIGNHGKGAAFQCLDCSSHNIKAVKIIEC"
     gene            complement(1062..1463)
                     /locus_tag="VGB68_02820"
     CDS             complement(1062..1463)
                     /locus_tag="VGB68_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048191.1"
                     /translation="MLNISNYLRNKLLDKAVSGTNFTPGANLFIRLFTTPLDRDAVGT
                     ELDNLGYTPLQVANNLTTFPAASSGAKANAVRFDFEPAEEDWNPILSIGIFDASTAGN
                     LYFYQNLDDALQIDDEQNLYFDIGDIQFEIL"
     gene            complement(1468..1674)
                     /locus_tag="VGB68_02825"
     CDS             complement(1468..1674)
                     /locus_tag="VGB68_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048192.1"
                     /translation="MFKRFINYFKALLPFLFASQLSPYYAPARSAGQFPNGRYMFYGR
                     TNGASRRRKSNRLRISKMTRNKHR"
     gene            complement(1703..2113)
                     /locus_tag="VGB68_02830"
     CDS             complement(1703..2113)
                     /locus_tag="VGB68_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048193.1"
                     /translation="MSRFYKIQLDSIYLTSDGTETGTPCKLEVPNAAALMLPFTGTTV
                     PAADGTPVTQILESVKGIAFEIKINVLTADVWEDVKTLIITALENSDTIEITATGDAG
                     DFTVEAEPVLPQPFLFESFINDRIKGITLRFMTA"
     gene            complement(2129..>4163)
                     /locus_tag="VGB68_02835"
     CDS             complement(2129..>4163)
                     /locus_tag="VGB68_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048194.1"
                     /translation="FLNLPKMNDDSLSVELLITDDKAEVVLSKNKQRLQDFARDAERI
                     QPQIKFKIPNENFIDLDKLEKKAAETRKQFQQVTSARLDAGNIGSLTREIINASERSR
                     QLTTDIANIKKELSNPNRKSSIAFLTDELRAAEKEAEQLDRKLNTLSSANLPAGKSTV
                     AGRSGAGGGKRGRVSDTAATFLELSDDFAPEGLNRAYNAATRSALAFNAVSMTSLGIF
                     GAIALAGYGIVKVTENIRTEAERRLQVEFQIQGAINNQILSQKEGLANSQKIREEAQK
                     AWEFNRFLNDSSVEELTRRKTNLERLQKMLPVTLPVVENGKVIAKPNEDYLKYDQQLL
                     ALDEQIRATRQRNAQTSNEAFDNRWKSYVKSQDDAREAEQKRLKQQNESIEKAKKQVL
                     DLEKTYNSVFESLYQKQGINNPFFQIFSESGKAANDLKVKLAGLNPELQAAALQMQKT
                     LNAKALLSARLDNRVDAFDLREQASNFRNPFDPEKLKTEEEEFIKRFLYNNPNYLYLK
                     NKKEVDADTRKDILSFGLFDTPQSRLNKSLDEKFDLIYGRGKLSPEQQATADRKFLSI
                     TQGVSPLELSNGNREQAALVREREAARRERYEQDAMALYREQRDLQKKIEANTARQNK
                     IAEEKGEQGIKMDITTDNGVKDVRQIKTPTAADTQSFYNPELNFTGGGGLTNR"
BASE COUNT         1066 a          886 c          995 g         1216 t
ORIGIN      
        1 aacacaatcg cgaaaaaata attgaagatt ataaaagtgg tttgtcttac aaagaaatac
       61 aagaaaaata cggcgtgaaa tacaaatggt ttcatcgcgt cttacggaac aacgccatag
      121 agttaagagg acataaaaga aaatttacac aagccgatga aaattcttta gtagaaaaat
      181 atcaaaacgg ctctggaatt aatgctctag tcagagaata taaaacttcc gccgtaacaa
      241 taaaaaatat tttaaataaa agagggattg accgaaaaaa aatcggcggt caacctcgta
      301 aatggtcaaa agaagatatt gaaaaagtta taaaattata tgattccggc aaaggtttat
      361 cacaacagaa aatcgctcaa ataatggaat ccgaccaaag aagaatcagt gttatcttac
      421 gtcagaacgg cgtatctaca aaaagaatct cggaacgaca tcctaattgg aaaggcggga
      481 gaattataga cgaaagcgga tatgtgcgag ttttagctaa atacgctgga aaattcgctt
      541 atgaaatgtc tttgtcagac ggatatgttt ttgagcatcg cgcagtaatg gctcaatatt
      601 taggcagacc gcttaggaaa aatgaaacgg ttcaccacat tgacggaaat agaagcaaca
      661 acaatattga aaatttgcag ttgagaatag gtaatcacgg caagggggcg gcgtttcaat
      721 gccttgattg cagttcgcat aatattaaag ccgtaaaaat tatagagtgt tagcttttac
      781 ttcgcgccaa ttttgcggaa ctaacagaaa aaatccggca aaataaaaaa actttggtgt
      841 aaaaacttaa aagataattt tccgacaacc tacctttact aacccgtttt tatactcgaa
      901 tcattgttca atttcaaaga aatttttgtt ttcaattaat tgcaactcac gcgccgcgag
      961 gcaataaacc aacccactga aaagcaaagg cttattgcgt tgtatgcact ttctctctcg
     1021 ccgcgaaaaa cacgatttac ttaaacaaac ttaaacaggc atcataggat ttcgaactgg
     1081 atgtcgccga tgtcgaaata aagattctgc tcgtcgtcga tttgcaaagc atcgtccaaa
     1141 ttttgataaa aataaagatt tcccgcggtc gaggcgtcga atatgccgat tgagagaatc
     1201 gggttccagt cttcctccgc cggttcgaaa tcgaagcgca cggcgtttgc tttcgcgccc
     1261 gaactcgctg ccggaaaggt cgtcagatta ttcgcaacct gaaggggcgt gtaacccaga
     1321 ttgtccagtt ccgtgccgac ggcgtcgcgg tcgagcggcg tcgtaaaaag tcgaatgaac
     1381 aggttcgccc caggcgtaaa attcgtcccg gaaacggctt tgtcgagcag cttgtttctg
     1441 aggtagttgc tgatgtttaa cataaggtta gcggtgctta tttctcgtca ttttcgagat
     1501 tctgagacgg ttcgatttgc ggcggcgcga agctccgttc gtgcgcccgt aaaacatata
     1561 gcgaccattc ggaaattgac cggctgaacg ggcgggtgcg taatacggcg aaagctgcga
     1621 ggcgaaaaga aacggcaaaa gagctttgaa ataattaatg aagcgtttga acattttcgt
     1681 gaaaattcct ttttttagat ttttaagcgg tcataaaacg cagcgtaatg cccttgattc
     1741 ggtcgttgat aaacgattca aagagaaacg gctgcggcag aacgggctct gcctcgactg
     1801 taaagtctcc ggcgtcaccg gttgctgtta tttcaattgt gtcggaattt tccaaagcgg
     1861 tgatgatgag cgttttgacg tcttcccaga cgtccgccgt caggacgttg attttgattt
     1921 caaaagctat tcctttgacg gattcaagta tttgcgtgac gggcgtgccg tcggctgccg
     1981 ggacggtcgt gccggtgaat gggagcatta aagcggcggc gtttggcact tccagcttgc
     2041 agggcgtgcc ggtttcagtg ccgtccgagg tgagataaat agaatcgagc tgaattttgt
     2101 aaaaacgtga catggtgagt tttattgatt accgattagt taagccgccg ccgcccgtaa
     2161 agtttaattc cggattgtag aaggattgag tgtccgcggc ggtcggggtt ttgatttgtc
     2221 tgacatcttt tacgccgttg tcggtcgtga tgtccatctt gatgccttgt tcgccctttt
     2281 cttcggcgat tttgttctgc cgcgcggtgt tggcttcgat tttcttttgc aaatcgcgct
     2341 gctcgcggta aagcgccatc gcgtcctgtt cgtagcgctc gcgccgcgcg gcttcgcgtt
     2401 cccggacgag cgccgcctgc tctcgattgc cgttcgacaa ttccagcgga ctgacgccct
     2461 gagtgatgct gagaaacttc cggtcggcgg ttgcctgctg ttcgggcgat agcttgcccc
     2521 tgccgtaaat caaatcgaac ttttcatcga gcgatttatt aagccgcgat tgaggcgtat
     2581 cgaacaaacc gaaggaaaga atgtcttttc gagtatcggc gtcgacttcc tttttatttt
     2641 ttagataaag ataattcgga ttgttgtaga gaaatcgttt gataaattcc tcttcttcgg
     2701 tttttaattt ttccgggtcg aaaggattgc ggaaattcga cgcctgctcg cgcaaatcga
     2761 aagcatcgac ccggttatcg agccgagcgg ataagagagc ctttgcgttt aaggttttct
     2821 gcatctgcaa agcggcggct tgcagttccg ggtttaagcc tgcgagtttg actttcaaat
     2881 cgttcgccgc ttttccgctt tccgagaata tttgaaaaaa cgggttgttt attccttgtt
     2941 tttgataaag actctcgaaa acggaattgt aagttttttc taaatccaga acttgctttt
     3001 tggctttttc gattgattca ttctgttgct ttaaccgttt ctgctctgct tcccttgcgt
     3061 cgtcttgcga tttgacgtag cttttccagc gattatcaaa ggcttcgttt gacgtttgcg
     3121 cgtttcgctg tcgagtagcg cgtatctgct catcgagagc gagaagctgt tggtcgtatt
     3181 ttagataatc ttcgttcggc ttggctatga ctttaccgtt ttctacgacc ggcagggtga
     3241 cgggtaacat tttttgaagc cgttcgaggt tggtttttct gcgcgtcagt tcttccacgc
     3301 tcgaatcgtt taaaaaacgg ttaaactccc acgccttctg cgcttcttca cggattttct
     3361 gcgaatttgc taatccttcc ttttgcgaca agatttgatt attgattgcg ccttgaattt
     3421 gaaattcaac ctgtaaacgc ctttctgctt ccgtgcgaat attttccgtg actttgacga
     3481 ttccgtatcc ggcgagggcg attgcgccga aaatacccag cgaggtcatc gaaacggcat
     3541 taaaagcaag tgcgcttcta gttgcggcgt tataagctcg gtttaagcct tcgggcgcga
     3601 aatcgtcact tagttctaaa aaagtcgcag ccgtgtccga aacccgtccg cgcttaccgc
     3661 cgcccgcgcc gcttcttccg gcgaccgtgc tttttccggc gggtaaattt gccgaagaca
     3721 aagtgtttaa ttttctatca agttgttcgg cttctttttc ggctgcgcgg agttcgtcgg
     3781 ttaaaaaagc gattgacgat tttcgattcg ggtttgataa ttccttttta atgttggcaa
     3841 tgtcagtagt aagctgtcgg ctgcgctcgc tcgcatttat aatttctctg gtcaaactgc
     3901 cgatattgcc agcgtcaaga cgcgcagaag tgacttgctg gaattgtttg cgcgtttctg
     3961 cggctttttt ttccagcttg tccaaatcaa taaaattctc gttcggaatt ttgaatttta
     4021 tttgcggctg gatacgttca gcatcgcggg cgaagtcctg caagcgttgt ttatttttac
     4081 ttaaaacaac ttctgcttta tcgtcggtaa ttagtagttc gacacttaga gaatcgtcat
     4141 tcattttagg caggttaagg aaa
//
LOCUS       DASWNE010000136         6271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_428283, whole genome shotgun sequence.
ACCESSION   DASWNE010000136 DASWNE010000000
VERSION     DASWNE010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6271
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428283"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1097
                     /locus_tag="VGB68_02840"
     CDS             <1..1097
                     /locus_tag="VGB68_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048195.1"
                     /translation="ISLVNRAGGARQTLIGGLPSGINRAEGQPAPSGPSGLKLNGLKL
                     YVTIAQGDAVLPGGRGGLQPNPNPATPLNDSILELTLPADYEALASGFALSFADQISL
                     VGGGTVTLLNAEDKALSVRMVANLPDWKREPVPNLPQYNVRASNVFGIESANGSLYAV
                     DASFNQLYRINPATGVYETFVVFPPKPNPLPFGPPVSEAVPNNVRLYGNRLLVSHLVG
                     FPFAAGVSEVREVNLQDGSHRSFAGGLTSAMDILPVAIPGDYDFFFVLEFSANMLGNP
                     TPPGRLKLYRFLKSDDSNPEGVDPVVLVSNLISPTSAARDGETGDLFVTEIFTGRIIR
                     VSATVNANTQMASNNQDVCAPCSFSLIKQN"
     gene            1135..1635
                     /locus_tag="VGB68_02845"
     CDS             1135..1635
                     /locus_tag="VGB68_02845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HEY0048196.1"
                     /translation="MYSKITNVFLILMFTAVSFYAQEKAKTSEASRDDAAIRANVEQM
                     AKGWNAKSGAEFAKPFAEDADYVIVNGMQIKGRAIIDKSHQDIFDTIYKNSTVSVSVE
                     QIRFLRPDVALVHVRSSLSVTRENSTRTTSGKITLVMTKNKDNWEVAAFQNTQIQAPG
                     NQAEKN"
     gene            1796..3301
                     /locus_tag="VGB68_02850"
     CDS             1796..3301
                     /locus_tag="VGB68_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048197.1"
                     /translation="MSINLTRDSLDGKIKSNNAATILRGGHYWIFKDADNPWRCLVKP
                     GEPLPFKMEYNAAPACQTNSFRVQELVWQSEPWTMLFEHMKMMNCNFLRIWLTGGTVV
                     AGTGAEPKPLDLTPFVRVKSGTKWKWQVYNAVVNNVWNEEYFRRLSSFAEKAENAGVV
                     LQISLFNYLDLSRRLDDGDFRGWCRSPWNPELSDHPPTLPNWAKNHLVNAGGTFYCTD
                     PVNNPAESERQAFFLEPTNKLRTIQQALVRKTVQTLAARTNIIYEIMNEPRGTHHQSA
                     AFGSVVTSWILNAAGSRRPLVSVNASNLTAAGIFDVDYWRDNQATIPNYDKLDAISYH
                     GLTGFDARKLAYCGANIDVPLVNPGSIKARFNKHRGGHKTKSLIYCTDAARTATHEFP
                     GVNGGVYELQTRDGQIWTNYPNVNSDTPQEQRKKSDLQNWAYWCLSLAVPNAGMVHFQ
                     NHSLDQVSYRRIGNALNEAQISPMSIEADESLETAAAAAAMAETAEAAPVA"
     gene            3500..>6271
                     /locus_tag="VGB68_02855"
     CDS             3500..>6271
                     /locus_tag="VGB68_02855"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0048198.1"
                     /translation="MNKCPQCSNIPSPAERRCRVCGAAVAPELAETLPLNFNEQPMNN
                     ERWQKIKSLFDAAQELDAKKREKFLENACAGDAELRREVEKLLASFENAESFLENPAA
                     AEVASMFEDKRTLVAHHLTGSRPPDEKFVAGTLLANRYRIIGLLGRGGMGEVFKAEDI
                     KLNQTVALKFLPDKLEKDKAALERFHAEVRNARQVSHGNVCRVFDIGEIEGSHFLSME
                     FIDGDDLSSLLRRIGRLPSDKAIEIARQLCFGLAAIHEAGILHRDLKPANVIIDSKGK
                     ARITDFGIAGLEAELKDGEIRVGTPAYMSPEQITGREVSAKSDIYSLGLLLYEIFTGK
                     QTFQADSIPDLLQKQQTTAPTNPSEVLKEINPLVERVILQCLEKNPEQRPKNALQVAM
                     MLPGGNPLEAAIAAGETPSPEMVAAAPKKGALRPVVAVSLLVCVFVSIGLLMLMSKET
                     SLHRLLRLEKSPEVLRERSRELAEKLGYRALDSHHGFIRELDYLDYLKEHDQSATRWR
                     KLANGQPAVAKFWYRQSPEPLVPLSSGTISVKDPPNAVPEMAQMYLDAKGRLIFFDGV
                     PPRVDESGEPTKKIDWAGVFREAGFNLEDFQETASQWSPPRAFDERRAWTGKYPEQSD
                     ISIRVEAAAYRGQLVQFEIVEPWTSPHGQAPYQGGVGTDISAVILLAIFFTVLFVSAW
                     LAIKNVRGGRSDVRGAFRVAVFLFVVRMLGWAFQTHHVASPDEVWLFLSGLQSGLFWS
                     CFVGLMYLSFEPYLRKYAPERVISWNRLLSGDWRNPLVGRDILIGGVAGLAVMVLVSL
                     RYFISIWFFGEPPLVPHSISNPGGAALLGIRGFPVLFLNQITASLVFAFMVSFLILFF
                     TLLLRHRWFGYAAFWLLLSSFAVSNDIGAGRPLSGMFWSVLFPTFLVLTAARFGVLAM
                     ASGFVFYHLV"
BASE COUNT         1415 a         1669 c         1802 g         1385 t
ORIGIN      
        1 gcatctcgct ggtcaatcgc gcaggcggag cgcgccagac tcttatcggc ggcttgcctt
       61 cgggcatcaa ccgcgctgaa gggcagcccg cgccgagcgg accgtcgggc ttaaagttaa
      121 acggattaaa gctttacgtg accatcgcgc agggcgacgc ggtcttgccg ggcgggcgcg
      181 gcggcttgca gccgaacccg aatccggcga cgccgcttaa cgattcgatt ttagagctga
      241 ctttgccggc ggattacgaa gctctggcga gcggcttcgc gctttctttc gccgaccaga
      301 tttcgctggt cggcggcggc acggtcacgc ttttaaacgc ggaagacaaa gcgctttcgg
      361 ttcgaatggt agcgaacctg cccgactgga agcgcgagcc cgtgccgaat ctgccgcaat
      421 acaacgtgcg cgcgtcgaac gttttcggca tcgagtcggc gaacggcagt ttgtatgcgg
      481 tcgatgccag cttcaaccag ctttacagga taaatccggc aacgggcgtc tatgaaacgt
      541 tcgtcgtttt tccgccgaag ccgaatcctc tgccgttcgg tccgcccgtt tccgaagccg
      601 tgccgaataa cgtccgcctt tacggaaatc gcctgctcgt ctcgcatctg gtcggattcc
      661 cgtttgcggc gggcgtgtcc gaagtgcgcg aagttaattt acaggacggc tcgcaccgga
      721 gtttcgccgg cggtctgacg tcggcgatgg acattctgcc ggtggcgatt ccgggcgatt
      781 acgatttttt ctttgtgctg gaattcagcg ccaatatgct cggcaacccg acgccgccgg
      841 gacgcctgaa gctttacaga tttctgaaaa gcgatgattc aaatcccgaa ggcgtcgacc
      901 cggttgtttt ggtcagcaac ctgatttcgc cgaccagcgc ggcgcgcgac ggcgaaaccg
      961 gcgatttatt cgtgaccgag atttttacgg gcagaatcat acgcgtttcg gcgaccgtca
     1021 atgcgaatac tcagatggca agcaacaatc aggatgtttg cgctccgtgc agcttttcgc
     1081 tcataaaaca aaattaatta cagaaaccaa aattaatcga aggagaaaat aaatatgtat
     1141 tcaaaaatta cgaacgtctt tttaattctg atgtttacgg ctgtcagctt ctatgcgcag
     1201 gaaaaagcga aaacttccga ggcgagcagg gacgacgcgg cgattcgcgc caatgtcgag
     1261 cagatggcga aaggctggaa cgcgaaaagc ggagccgaat ttgccaagcc ttttgccgaa
     1321 gatgccgatt acgtcattgt caacggaatg caaatcaaag ggcgcgcgat aatcgataag
     1381 agtcatcaag acattttcga caccatttac aaaaattcta ctgtcagcgt gagcgtcgag
     1441 cagattcgtt ttctgcgtcc ggatgtcgcc cttgttcacg tccgctcgtc gctgagcgtg
     1501 acgcgggaaa attcgacgcg cacgaccagc gggaaaatta cgctggtgat gacgaaaaat
     1561 aaagacaact gggaagtcgc cgcgtttcaa aacacgcaga ttcaagcgcc gggaaaccag
     1621 gctgagaaaa attgaccgac accggcaatc aatcgccgga tttataagcc gtctcaagaa
     1681 caatttgagc cttgggcagc gacggctcaa accgtaaatt taccggctta tcgcttccgc
     1741 acctaaaacg gacttcgttt aaaaagaagc cgaaataaat taaaaggaga tagaaatgag
     1801 catcaattta accagagatt cgctggacgg aaaaattaag agcaacaacg ccgcgaccat
     1861 tctcagaggc ggtcattact ggatttttaa ggacgcggat aatccgtggc gctgtctggt
     1921 caaaccgggc gagccgcttc ctttcaagat ggagtacaac gcggctccgg cgtgtcagac
     1981 aaacagtttt cgggttcagg aattagtgtg gcagtcagag ccctggacga tgttgtttga
     2041 gcatatgaag atgatgaact gcaattttct gcgtatctgg ctgacgggcg gaacggtcgt
     2101 cgccggtacg ggagccgagc cgaaaccgct tgatttgacg ccttttgtgc gcgtcaaatc
     2161 gggcacgaaa tggaaatggc aggtttataa cgcggtcgtc aataacgtct ggaacgagga
     2221 gtattttcgt cgcttgagca gctttgccga aaaggctgaa aacgccggcg tcgtcctgca
     2281 aatctcgctg ttcaactatc tggatttatc gagacgtctg gacgacggcg attttcgcgg
     2341 gtggtgcaga agtccctgga atccggaatt gagcgaccat ccgccgacgc tgccgaactg
     2401 ggcgaaaaat catctcgtca acgcgggcgg aaccttttac tgcaccgacc cggtaaacaa
     2461 tccggcggaa agcgagcggc aggctttttt cctcgagccg accaataagc tcagaaccat
     2521 ccagcaggcg cttgtcagaa agaccgtgca aacgctcgcc gcgcgaacca acattatcta
     2581 cgagattatg aacgagccgc gcggcacgca tcaccaatcc gccgccttcg gctcggtcgt
     2641 cacgagctgg atactgaacg cggcgggcag ccgccgaccg ctcgtcagcg tcaacgcttc
     2701 gaatcttacc gccgccggca ttttcgatgt ggattactgg cgcgataatc aggcgaccat
     2761 tcccaattac gacaaactgg acgcgataag ctatcacggt ctgaccggct ttgatgcgag
     2821 gaagctggct tactgcggcg cgaatatcga cgttccgctg gttaacccgg gaagcattaa
     2881 ggcgcgcttc aataaacacc gtgggggaca caaaaccaaa tcgcttatct attgcacgga
     2941 cgcggcgcgg acggcgacgc acgagtttcc cggcgttaac ggcggcgtct atgagctgca
     3001 aacgcgcgac ggtcagattt ggacgaatta tccgaacgta aattccgaca cgccgcaaga
     3061 gcagcggaaa aaatccgatt tgcaaaactg ggcttactgg tgtctgagcc tcgccgttcc
     3121 gaatgccggt atggttcatt tccagaatca cagtctggat caagtcagct atcgccgaat
     3181 cggcaacgcg ctgaacgaag cgcagatttc gccgatgagc atagaggcgg acgagtcgct
     3241 ggagacagcg gcggcggcgg cggcgatggc tgaaactgcg gaagcggcgc cggttgcctg
     3301 acgaaggcgt ccttgattga gcaatgtccc gattctgagt cggcgtccgt cgaaaaagcg
     3361 aacgagcgcg agcgcatagt ttgcgaaggc aaacatcgcc gacccggaat cgggacattg
     3421 cccgtcgacg aaaaaactta caaaaaaatg aaaaaagtat tatcattgct gacaattttc
     3481 cgttcgaaat ctttttccga tgaacaaatg cccgcaatgc tcaaacattc cgtcgccagc
     3541 cgagcgccga tgccgcgttt gcggcgcggc ggttgcgccg gaattggcgg agacgctgcc
     3601 gctgaatttt aacgaacaac cgatgaacaa cgaacgctgg caaaaaatca aaagcctgtt
     3661 tgacgccgcg caggagctgg acgcgaaaaa gcgcgaaaaa tttctcgaaa acgcctgcgc
     3721 gggcgacgcc gagctgcgcc gcgaagtcga aaagctgctc gcttcttttg aaaacgccga
     3781 aagctttctc gaaaatccgg cagccgccga agtggcgagt atgtttgaag ataaaagaac
     3841 gctcgtcgcg catcacctga cgggcagccg cccgccggac gaaaaattcg tcgccggaac
     3901 gcttctggca aatcgttacc ggataatcgg cttgctcggg cgcggcggaa tgggcgaagt
     3961 tttcaaagcc gaagatatta aattaaatca gacggtcgcg ctcaaatttc tgcccgataa
     4021 attagaaaaa gataaagccg cgctcgaaag atttcacgcc gaagtgcgca acgctcgtca
     4081 ggtctctcac ggaaatgtct gccgcgtctt cgacatcggc gaaatcgagg gttctcattt
     4141 tctgtcgatg gaatttatcg acggcgatga tttgtcctcg cttttaagaa gaatcggacg
     4201 cctgccttcg gataaagcca tcgagattgc gcgccagctc tgtttcggtc tggcggcgat
     4261 tcacgaggcg ggaattctgc accgcgattt aaagcccgcc aatgtcatta tcgacagcaa
     4321 aggcaaggcg cgaatcacgg atttcggcat cgccggtctg gaagcggagc tgaaggacgg
     4381 cgagattcgt gtcggaactc ccgcctatat gtcgcccgaa cagattacgg gcagagaggt
     4441 ttccgcgaaa agcgatattt attcgctggg gcttctgctc tatgaaattt ttacgggaaa
     4501 acaaaccttt caagccgatt cgattcccga ccttttgcag aaacagcaga cgaccgcgcc
     4561 gaccaatccg tcggaagtgc tgaaggaaat caatcctctg gtcgagcgtg tgattctgca
     4621 atgtctggaa aaaaatccgg aacagcgccc gaaaaacgct ttgcaggtgg cgatgatgct
     4681 gccgggcggc aatcctctgg aggcggcaat cgcggcgggc gagacgccca gcccggaaat
     4741 ggtcgccgcc gctccgaaaa aaggcgcgct gcgtcccgtc gtcgccgttt ctctgctcgt
     4801 ttgcgtgttt gtttccatcg ggcttttgat gctgatgtcg aaagaaactt cgctgcatcg
     4861 ccttttgcgg cttgaaaaat cgcccgaggt gctgcgcgag cgcagccgcg agctggcgga
     4921 aaagctcggc taccgggcgc tggattcgca tcacggtttt atccgcgaac tggattatct
     4981 ggattatctg aaagagcacg accagagcgc gacgcggtgg cggaaactgg cgaacgggca
     5041 gccggcagtc gctaaattct ggtaccggca gagccccgag ccgctcgtcc cgctttcgag
     5101 cggcacgatt tcggtcaaag acccgccgaa cgccgtcccg gaaatggcgc agatgtatct
     5161 cgatgccaaa gggcgtttga ttttttttga cggcgtgccg ccgcgcgtcg acgaatcggg
     5221 cgagccgaca aaaaaaatcg attgggcggg cgtttttaga gaagccggtt tcaatctcga
     5281 agattttcag gaaaccgcgt cccaatggtc gccgccgcga gcctttgacg agcgacgcgc
     5341 gtggacgggc aaatacccgg aacaatccga catatccatc cgcgtcgaag ccgccgctta
     5401 tcgcgggcaa ttggtgcagt ttgaaatcgt cgagccgtgg acgagcccgc acgggcaggc
     5461 tccgtatcag ggcggcgtcg gcacggatat ttccgccgtc attctgctgg cgattttttt
     5521 cacggtttta tttgtcagcg cgtggctggc gattaaaaac gtgcgcggcg ggcgcagcga
     5581 cgtgcgcggc gcgttccggg tcgcggtttt tttatttgtg gtgcgaatgc ttggctgggc
     5641 gtttcagacg catcacgtcg cttcgccgga cgaggtgtgg ctttttcttt caggcttgca
     5701 gagcgggctt ttctggtcgt gttttgtcgg gctgatgtat ttatcattcg agccttacct
     5761 gagaaaatac gcgcccgagc gcgttatctc ctggaatcgg ctgctgtccg gcgactggcg
     5821 caacccgctg gtcgggcgcg atattttaat cggcggcgtt gccggtttag ccgtgatggt
     5881 tttggtcagc ctgcgctatt tcatttccat ctggtttttc ggcgagccgc cgctcgttcc
     5941 gcacagcatc tcgaatcccg gcggcgcggc gctgctgggc attcgcggct ttccggtttt
     6001 gtttttgaat caaatcaccg cgtcgctcgt tttcgccttt atggtttcgt ttctgatttt
     6061 gtttttcacg ctgcttttgc ggcacagatg gttcggctac gctgctttct ggctgttgct
     6121 ttcaagcttt gcggtctcaa acgacatcgg cgcgggcaga cctctgagcg gtatgttctg
     6181 gtcggtgctg ttcccgacat ttctggtttt gacggcggcg cgtttcggcg ttctggcaat
     6241 ggcttccggt ttcgtttttt atcatctcgt t
//
LOCUS       DASWNE010000137         6446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_534448, whole genome shotgun sequence.
ACCESSION   DASWNE010000137 DASWNE010000000
VERSION     DASWNE010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6446
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534448"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="VGB68_02860"
     CDS             complement(<1..905)
                     /locus_tag="VGB68_02860"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY0048199.1"
                     /translation="MLSVGAGLAQEFPCITQNRQIKIVKASDKSAAEECRNRRRNAKT
                     RQNRGIDNITIAGHVTHQNGVRMVGVTVTLENLTSGVIRTIVTGDEGTYFFNNLQFAD
                     DYEITPSRQGYEFYPPSVVVKGLVYDEVFNFIAAGPPPNPPTPPANQPTLAWTSYYDN
                     PLPPGSQSEPNADYNAMMARDAQGNVYVAGTSYTENYEKTGNTDISLYKTDANGNRVW
                     ARAFDGAGHYKDGAVDLGVDAAGNVYVGGYSWQSQNSDYDYVVVKYDANGNLLWSKYY
                     NNGGEDFPRSLKVDMAGNTYITGYS"
     gene            complement(1133..1708)
                     /locus_tag="VGB68_02865"
     CDS             complement(1133..1708)
                     /locus_tag="VGB68_02865"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY0048200.1"
                     /translation="MSSIITIPNLLTFLRMALIPVFASLLFYEYVALALLAFIVAGVS
                     DGVDGFIARRFKQESELGTILDPIADKLLMTTAFIILTLPNVLESNHRVPFWVTAAVI
                     GRDVLIIAVAGAINIMTGFHGFKPSWLGKLSTLVQVGAVVLILLAAVSGYGFFLPVTY
                     SIVVLLAFISGFHYIFHVARLMKEAEKTSKV"
     gene            complement(1837..2697)
                     /locus_tag="VGB68_02870"
     CDS             complement(1837..2697)
                     /locus_tag="VGB68_02870"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0048201.1"
                     /translation="MRISGTERVKLSQKLSAAVASDKPLRTLASGFSQFAREALAEAN
                     SISVENIKIKLKRLPKELDGFRLVHLSDIHHSPFTSLEHISRVVEVSNRLKPDMFVLT
                     GDYVSHESEYIAPVAEVLAQLKAEYGIYACLGNHDHWTDAELVTHLFRGEGINLLINE
                     GFRFEARNTKFWLAGVDDLMVGKTDVRAALDGSNEDEFKILLAHNPQIVRRAARYDVD
                     LMLSGHTHGGQVKLRDEEKRILPRRRLSSGLHRRQNTQVYITRGIGTVVLPVRYQCPP
                     EVSVIELCCN"
     gene            complement(2851..4674)
                     /locus_tag="VGB68_02875"
     CDS             complement(2851..4674)
                     /locus_tag="VGB68_02875"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEY0048202.1"
                     /translation="MDPITITLLLLLVALVLFASEKLPVDVVGIILVIGLIMTGILTV
                     QEGLQGFGDNIIITIAGLFILTGGLIKTGLVDLIGRRLYRLAGHNEFLLTTLIMLVAA
                     ACASVLKNTTTTAMFLPVVIGLSERAKIPPSKLLMPLAFGAILGGSCTLIGTSTNLAV
                     SSALSRYQQSPDFPEHIKPLLEPFSMFELTSVGAAAAFAGLLYMLVIGLRLLPSRGGE
                     ESFTEQYRIREYISEVLVLPGSHLIGKTLGEADINRELDLNVLGIIRGKEERIAPTAK
                     ERIELNDLLIVEGKIADILRIKSEAGLEIKPDFKLNDRVLEGGEIELFEVMVMRDSVL
                     VGQSLKSVGFRNKYNLTVLAATRHNETIFEKLSTMKLRFGDVLLVQGRRTNIEPMVTS
                     GDLLFLEEVSTGNMRTEKRKWAIAAFGLFLALSLSKVVTGFDVPLAVAVLLGVLLLLA
                     TQTIRHAELYTLIDFRLLVLIACMISFGVAMENTGADIYLAGLIQTHFGAYGATAVLA
                     GFFILTVALTQPMSNQAAALVVLPVAVKTAVVLGLNPRTFAVAVTYAASCSFLTPLEP
                     ACVLVYTPGRYRFFDFVKIGSLLTVIVFIVVILLVPLVWRF"
     gene            complement(4877..>6446)
                     /locus_tag="VGB68_02880"
     CDS             complement(4877..>6446)
                     /locus_tag="VGB68_02880"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0048203.1"
                     /translation="GVIETVPKRGYRFVASVRLMQDEENEPLVIHEKIRARILIDDAS
                     ERGGEWETRGRGDEIPNLNLENQIAASPPLPLSASQFPAPETHYAQSGDLNIAYQVVG
                     SGDLDLVFVMGWVSHLEYFWKEPHFAAFLKRLANFSRLILFDKRGTGLSDRVPLSELP
                     TLEQRMDDVRAVMDAVGSERAVLIGVSEGGPMCSLFAATYPEKTTALVMIGTYAKRIR
                     DEDYPWGVSLEDREKFFDLMRNDWGKPVGIEERAPTLANDEAFRDWWATYLRMGASPG
                     AAVALTKMNAEIDVRSVLPTIRVPSLVIHRAGDMCLKVEEGRFVAARIPGCKYVELGG
                     IDHLPFVGAQDEILDEIEIFLTGVRHDEQHDRVLATVMSVKIVDPHEQAVEVTNWEDL
                     MERSGVYVRRQIELFKGREVSFDENGLLATFDGPARAIRCAMAITDSARRLNIRVKTG
                     VHTGECDVRGGRFGGVAVELAEKIAEEAALGEVLASRTVKDLVAGSGLKFADYGAKSF
                     ADIQGEWRLFSVER"
BASE COUNT         1567 a         1788 c         1670 g         1421 t
ORIGIN      
        1 cacgaatagc ctgtaatata agtgttgccc gccatatcga ctttcagcga gcgcgggaaa
       61 tcttcgccgc cgttgttgta gtatttcgac cagagaagat ttccgttcgc gtcgtatttc
      121 acgacgacgt aatcgtaatc gctgttttga ctctgccacg aataaccgcc gacatagacg
      181 tttccggcgg catcgacgcc caaatcgacc gcgccgtcct tgtaatgccc cgcgccgtca
      241 aaagctcgcg cccagacgcg gttgccgttg gcgtccgttt tgtataaaga gatgtccgta
      301 ttgccggtct tttcgtaatt ttcagtgtag gaagttccgg cgacatagac gttgccctgc
      361 gcgtcgcgcg ccatcatcgc gttataatcg gcgttaggct cggactgcga gccgggcggc
      421 agcggattat cgtaatagct cgtccaggcg agtgtcggtt gatttgccgg cggagtcgga
      481 ggattcggcg gcggtcccgc cgcgataaaa ttaaaaactt cgtcgtagac gagtccttta
      541 acgacgaccg aaggcggata aaattcgtag ccctgccggg aaggcgttat ttcataatcg
      601 tcggcaaatt gcagattatt gaaaaaatat gtgccttcat cgcccgtaac gattgtgcga
      661 atcactcccg aagtgagatt ttccagcgtc accgtgacgc cgaccattcg gacgccgttc
      721 tggtgcgtga cgtgaccggc aatcgtaata ttgtcgatgc ctctattttg ccttgtcttc
      781 gcgtttcgac ggcgattccg gcattcttca gccgccgatt tatccgaagc tttaacgatt
      841 tttatctgtc tgttttgcgt aatgcacgga aattcctgtg ccaagcctgc cccgacggaa
      901 agcattaaaa agcctccgac taaaattgcg cgaacgaaac tgggaaacat ttgccactcc
      961 ttaaaaatta gttatttaaa gctctattaa ataaccgccg gtttaaccgc gaaatagaga
     1021 cgacccgcca aatacgcgtc acgagccgtg cccgcaataa tcagtttaaa aaaaaatctt
     1081 ttgatttttt tatacagtct cggagtggaa ttaaaaaaga cagtttcgga gattaaactt
     1141 tagatgtttt ttcggcttcc ttcatcaatc gcgcgacgtg aaagatatag tgaaaacccg
     1201 aaataaaagc cagaagcacg acgatggaat aagtcacggg cagaaaaaat ccgtagccgg
     1261 aaacggcggc gagaagaatt aaaacgacgg cgccgacctg cacgagcgtg ctcagcttgc
     1321 cgagccacga cggtttgaaa ccgtgaaatc cggtcataat attgattgcg cccgcgacgg
     1381 cgattatcaa aacgtcgcgc ccgatgacgg cggcggtcac ccagaacggc actcgatgat
     1441 tcgactccaa aacgttcggc agcgtgagaa tgataaacgc ggtcgtcatc aaaagcttgt
     1501 cggcaatcgg gtcgaggatt gttccgagct cggattcctg tttaaagcgg cgcgcgataa
     1561 agccgtcgac gccgtcggaa acgcccgcga cgataaaggc gagtagagcc agagccacat
     1621 attcgtaaaa aagcaggctg gcgaaaacgg gaataagagc cattcgcaga aaggtgagta
     1681 aatttggaat ggttatgatg cttgacatcg acggaatttg cgatttcaga tttcagattt
     1741 gcgattgccg ctattaaaaa ataattctag cccgaatctt tccaaaacca aaacaggcaa
     1801 acatttctca gccgctctaa aatctaaaat tacaaattaa ttacaacaaa gctcgattac
     1861 cgaaacttcg ggcgggcatt gatagcgaac gggcagaacg accgttccga tgccgcgcgt
     1921 gatataaacc tgcgtgttct ggcggcgatg caagccgctc gaaaggcgtc ggcgcggcag
     1981 aattcttttt tcctcgtcgc gcagcttgac ctgcccgccg tgcgtgtgtc cgctgagcat
     2041 caaatcgacg tcgtatctcg ccgcccggcg cacgatttgc ggattatgcg cgagcagaat
     2101 cttaaattcg tcttcattcg agccgtcgag agcggcgcgc acgtccgttt ttccgaccat
     2161 caaatcgtcg acgccggcga gccagaattt cgtgttgcgc gcttcgaagc gaaacccttc
     2221 gttgattaaa agattgattc cctcgccgcg aaacaggtgc gtgaccagct cggcgtcggt
     2281 ccaatggtcg tggttgccta aacaggcgta aattccgtat tcggctttca gctgcgccag
     2341 aacttcggcc accggcgcga tgtattcgct ttcgtgcgaa acgtaatcgc cggtcagcac
     2401 gaacatatcc ggctttaaac gattggaaac ctcgacgacg cgcgagatgt gttcgaggct
     2461 cgtaaaaggg ctgtgatgaa tatcggaaag atgaaccaga cgaaagccgt ccagctcttt
     2521 cggcaggcgt ttcagcttga ttttgatgtt ttcgacggaa atcgaattgg cttccgccag
     2581 agcctcgcgc gcaaactgcg aaaacccgct cgccagcgtg cgcagaggtt tatcggaagc
     2641 gaccgccgcc gacagttttt gagacagttt tactctttcc gttcctgaaa ttctcattag
     2701 tgaattttag atgctgaaaa acttaggtat gactgctgta aaaattatag ctaataaagt
     2761 ggaaaagtgg aaaagtggaa aggtgaaaaa gcaatcgtcg atttcccgcc gaccgacttt
     2821 ttcgcttttt caccctttta ccctttcact ctaaaatctc cagaccagcg gcactaataa
     2881 aatgacgacg ataaaaacaa tcacggtcag cagcgagccg attttgacga aatcgaaaaa
     2941 gcggtagcgt ccgggcgtgt aaaccagaac gcacgccggt tcgagcggcg ttaaaaacga
     3001 gcaggacgcg gcgtaagtca ccgcgacggc aaaagttcgg ggatttaagc cgagcaccac
     3061 cgccgttttc accgcgaccg gcagaacgac gagcgcggcg gcttgattcg acatcggctg
     3121 cgtcagcgcc accgtcagaa taaaaaatcc ggcgagcacc gccgtcgcgc cgtaggcgcc
     3181 gaaatgcgtc tgaatcaagc ccgccagata aatgtcggcg cccgtgtttt ccatcgccac
     3241 gccgaagcta atcatacagg cgattaaaac cagcaggcga aaatcaatca gagtgtaaag
     3301 ctcggcgtgg cgaatcgtct gtgtagccag cagcagcaga acgccgagca gaaccgcgac
     3361 cgccagcgga acatcgaagc cggtgacgac cttcgacagc gacagcgcca gaaacaagcc
     3421 gaacgcggca atcgcccatt tgcgtttttc ggttcgcata tttccggtcg aaacttcttc
     3481 cagaaacaat aaatcaccgc ttgtgaccat cggctcgata ttcgttcgcc tgccctgcac
     3541 gagcaaaacg tcgccgaaac ggagcttcat cgtgctgagc ttttcaaaaa tcgtctcgtt
     3601 gtgacgcgtc gccgccaaga cggtcagatt gtatttgttg cgaaagccga cgcttttcag
     3661 gctctgcccg accagcaccg aatcgcgcat taccatcact tcgaaaagct cgatttcgcc
     3721 gccttccagc acgcggtcgt ttaatttaaa atcgggtttt atctccagtc cggcttcgga
     3781 tttaatgcgc agaatgtcgg cgatttttcc ttcgacaatt aacaagtcgt tcagctcgat
     3841 gcgctctttg gcggtcggcg cgattctttc ttccttgccg cgaatgatgc cgagcacgtt
     3901 taaatccagc tcgcggttga tgtcggcttc gcccagcgtt ttgccgatca gatgcgagcc
     3961 gggcaaaacg agaacctcgg aaatatattc gcggattcga tactgctcgg taaaagattc
     4021 ctcgccgccg cggctcggca aaagccgcaa gccgatgacg agcatataga gcagcccggc
     4081 aaaagccgcc gcggcgccga cgctcgttaa ttcgaacatc gaaaaaggct ctaataaagg
     4141 cttgatatgc tcggggaaat cggggctttg ctggtaacgc gaaagcgcgc tgctgaccgc
     4201 cagattggtc gaagttccga taagcgtgca gcttccgccc agaatcgcgc cgaaagcgag
     4261 cggcatcagc agtttcgacg gcggaatttt ggcgcgttcc gaaagcccga tgacgacggg
     4321 cagaaacatc gcggtcgtcg tcgtattttt cagaaccgac gcgcaagccg ccgcgaccag
     4381 cattatcagt gtcgtcagca aaaactcgtt gtgaccggcg aggcggtaaa gcctgcgccc
     4441 gattaaatcg accaagcccg tttttatcaa cccgcccgtc agaataaaaa gcccggcgat
     4501 ggtgatgata atattatcgc caaagccctg taagccttcc tgcacggtca ggatgccggt
     4561 cattatcaaa ccgataacca gaataatacc gacgacatcg acggggagtt tctcgctggc
     4621 gaaaagcaca agggcaacca gcaggagaag aagtgtaatc gttattggat ccatttcgga
     4681 aaggataccg aaattcggcg cgatattcca gacgtttcca tacaaactaa attattcgtc
     4741 ctttaatttc atacggaatg acgaatggaa agaaaattac ggatgacgag cgacgaatga
     4801 cttcgagcgc ctgttcggtc tgagttgcag gcgatatatt ctttatctca gcacttaaaa
     4861 ctcagcgctt ttccttctat ctttccacgc tgaaaagccg ccattcgccc tgaatatcgg
     4921 caaaagattt cgcgccgtaa tcggcgaatt tcaagccgga accggcgacc aaatccttca
     4981 ccgtccgcga agccagcact tcgccgagcg ctgcctcttc cgcaattttt tccgccagtt
     5041 cgaccgcgac gccgccgaat ctgccgccgc gcacgtcgca ttcgccggtg tgcactccgg
     5101 ttttgacgcg aatattcaag cgccgcgccg aatcggtaat cgccatcgcg cagcgtatcg
     5161 cgcgcgccgg tccgtcgaag gtcgccagca agccgttttc gtcaaaggaa acctcgcgcc
     5221 ctttgaaaag ctcgatttgg cggcggacgt aaacgcccga gcgctccatt aaatcttccc
     5281 agttcgtcac ttcgacggct tgctcgtgcg ggtcgacgat tttcacgctc atcaccgtcg
     5341 ccagcactcg gtcgtgctgc tcgtcgtggc gcacgccggt cagaaatatt tcgatttcgt
     5401 cgagaatctc gtcctgcgcg ccgacaaacg gcagatggtc gatgccgccc agctcgacgt
     5461 atttgcagcc cggaatccgc gcggcgacaa agcgtccttc ttcgactttc agacacatat
     5521 cgcccgcgcg gtgaatgacc agcgacggca cgcgaatcgt cggcagcacg ctgcgaacgt
     5581 cgatttcggc gttcattttc gtcaaagcga ccgccgcgcc cggacttgcg cccatccgca
     5641 ggtaagtcgc ccaccaatcg cgaaaagcct cgtcatttgc cagcgtcggc gcgcgttctt
     5701 cgatgccgac gggcttgccc caatcgttgc gcatcaaatc gaaaaacttt tcgcggtctt
     5761 ccaggctgac gccccaggga taatcttcgt cccggatgcg cttggcgtat gttccaatca
     5821 tcaccagcgc cgtcgttttt tcgggatagg tcgcggcaaa aagcgagcac atcggaccgc
     5881 cctcggaaac gcctatcagg acggctctct ccgaaccgac cgcgtccatc acggcgcgca
     5941 cgtcgtccat tctttgttcg agcgtcggca gctccgacaa aggcactcgg tctgacaaac
     6001 ccgtcccgcg tttatcaaat aaaatcaggc gcgagaaatt ggcgagccgc ttcaaaaacg
     6061 cggcgaaatg cggctctttc cagaaatatt cgagatgcga aacccagccc atcacaaaaa
     6121 ctaggtccaa atccccgctg ccgacaacct gataagcgat gttaaggtcg ccgctctgcg
     6181 cgtagtgcgt ctccggcgcg gggaattgag acgcggagag cgggagaggc ggcgacgcgg
     6241 cgatttgatt ttcgagattc aagttcggaa tttcatctcc gcgtccgcgc gtctcccact
     6301 ctccgccgcg ctcggaagca tcgtcaatca aaatgcgggc gcggattttt tcgtggatga
     6361 cgagcggctc gttctcttcg tcctgcatta ggcgaaccga cgcgacgaag cgatagccgc
     6421 gtttcggaac cgtttcgata acgcct
//
LOCUS       DASWNE010000138         2635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_605005, whole genome shotgun sequence.
ACCESSION   DASWNE010000138 DASWNE010000000
VERSION     DASWNE010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605005"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1800)
                     /locus_tag="VGB68_02885"
     CDS             complement(<1..1800)
                     /locus_tag="VGB68_02885"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0048204.1"
                     /translation="MGISLSSSNTTHLITALGILAFSQFLSSTSFATMYYYLKKDMNL
                     WQAWKKEGFSSSLTQIAGAAMAGVIFKLINYANPIATAIALFAVGVAYLNYRRMIFEI
                     NESIEQVEKAEREKAEVERTRAEQAEKYIEELNDLLHGQERISDQLRESKDLFEHAAL
                     HDMLTRLANRAFLIEKLEFLLALNKNNSSARFYVLFLDLTRFKNINDSLGHTIGDKVL
                     MLVAKRLLRVVNPEDTVARLGGDEFAIILSDPLSVSHAEKIAHDIYQKLTQPFSLRGN
                     RIFTKLHIGLAAYDPDYKKPEDILRDADIAMHHAKEKGLEVAVFDKDLRARFLESVGL
                     EQDLRYAIDRNELSMFYQPLISLKNGEIVGFEALLRWQHSKRGFVPPVKFIPIAEDSG
                     LIIPITEWILKDTARQIAVWQKISPAYRKLIVSVNISGKHLAVEGLVEQVSKTLEITK
                     IRPSTLKLEITESTAMENAERSMQILNRLKELGVQLSIDDFGTGFSSLSQLHRLPFDT
                     LKIDRSFVNAVGENGENSEILQTIISLAKNLKMKVIAEGIETENQLSLLRNLGCDYAQ
                     GYLLAKPMPCAEMEKLLYQKHFWFPESFTADDSH"
     gene            1841..2239
                     /locus_tag="VGB68_02890"
     CDS             1841..2239
                     /locus_tag="VGB68_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048205.1"
                     /translation="MARAVIPKFNTITVSVNLLRSFSFKNKQLAITSRGARTIAASPP
                     KSRNVRNITGSEKLSEKFERGIVKLILGAIISVKITKRTKLQLKTFESKLKIAKVNDK
                     PPSRMTANLKNVRTFTRFIKREPDRQLGSL"
BASE COUNT          743 a          581 c          631 g          680 t
ORIGIN      
        1 gtgcgaatcg tccgcggtaa aggattcggg aaaccagaaa tgtttctggt agagaagttt
       61 ttccatctcg gcgcacggca tcggtttggc gagcaggtag ccctgagcgt aatcgcagcc
      121 gagattacgc agcagcgaca attgattttc ggtttcgatg ccttcggcga tgactttcat
      181 cttcaggttt ttggcgagcg agataatcgt ctgaagaatc tcggagtttt cgccgttttc
      241 gccgacggcg ttgacaaacg agcggtcgat tttcagagtg tcgaacggca gccggtggag
      301 ctggctgagg ctcgaaaatc cggttccgaa atcgtcaatc gaaagctgga cgccgagttc
      361 cttgaggcga ttgagaatct gcatcgagcg ctcggcgttt tccatcgccg tgctttccgt
      421 tatttccagc tttaaggtag aaggtctgat tttagtgatt tccagagtct tggaaacctg
      481 ttcgaccaag ccttcgaccg ccagatgctt gccggagatg ttgacgctca caatcagttt
      541 tctataagcc ggcgaaattt tctgccagac ggcaatctgg cgggcggtgt ctttcagaat
      601 ccattcggta atcgggataa tcaagccgga atcttcggct atcgggataa atttgacggg
      661 cggaacaaag ccgcgcttgg aatgctgcca gcgcaaaagc gcttcaaaac cgacaatctc
      721 gccgtttttc agagagatta aaggctgata gaacatcgaa agctcgttgc ggtcgatggc
      781 gtagcgcaag tcctgttcga gcccgactga ttccagaaag cgagcgcgca aatctttatc
      841 gaaaaccgcc acctccaagc ctttctcttt ggcgtgatgc atggcgatgt cggcgtcgcg
      901 cagaatgtct tccggttttt tgtaatccgg gtcataagct gccagtccga tatgcagctt
      961 ggtaaaaatg cgattgccgc gcagcgaaaa aggctgcgtt agtttctggt aaatatcgtg
     1021 cgctatcttt tccgcgtggc tgacggaaag cgggtcgctc aggataatcg cgaattcgtc
     1081 gccgccgagc cgcgccaccg tatcttccgg gttgaccacg cgcaaaagcc gcttggcgac
     1141 cagcatcaga accttgtcgc cgatggtatg accgaggctg tcgttgatgt ttttaaagcg
     1201 cgttaaatcc agaaacagaa cataaaaacg ggctgatgaa ttatttttgt tgagagcgag
     1261 caaaaattcg agcttttcga ttaaaaaagc gcgattggca agcctggtga gcatatcgtg
     1321 cagcgctgcg tgctcaaata aatccttgct ttcgcgaagc tggtcgctga ttctttcctg
     1381 accgtgaagc aggtcgttta attcttcgat atatttttcg gcttgctcgg cgcgcgttct
     1441 ttcgacttcg gctttttctc gctcggcttt ttcgacttgc tcgatggatt cgttgatttc
     1501 gaaaatcatc cgccggtaat tcaggtaagc tactccgacc gcaaacaaag cgatggcggt
     1561 cgcaatcggg ttggcgtaat ttatcagctt gaagataacg ccagccatcg ccgcaccggc
     1621 aatctgcgtc agggagctgc tgaaaccttc ttttttccaa gcctgccaaa gattcatatc
     1681 ttttttcaga tagtaataca tcgtagcaaa gctggtgctg ctcagaaatt gtgaaaaggc
     1741 aagaataccc agcgccgtta ttagatgggt cgtgtttgat gaagacagag aaattcccaa
     1801 aaaatttgga atagttagcc aaacgaagta actaatagtt gtggcaagag ccgttattcc
     1861 aaaatttaac acgataacag tgagcgtaaa cctgttacgc tctttcagct ttaagaataa
     1921 gcaactggca attacttcaa gaggagccag gacgattgcc gcttcgccgc cgaaaagcag
     1981 aaatgtcaga aatataaccg ggtcggaaaa actcagcgag aaattcgagc gaggcatcgt
     2041 caaactcatt ctcggagcta taatcagcgt aaaaataact aaaagaacaa aactccagct
     2101 aaaaaccttt gagtctaaat taaaaattgc aaaagtaaat gacaaaccgc caagtaggat
     2161 gacagccaac ttaaaaaatg tcagaacttt tactcgattc ataaaaagag agcccgacag
     2221 gcaattagga agtctttaaa gttttgcagg gaatgctagg aaaacagctt agtgaagcac
     2281 tcggatgggt ttaagaaatc tgacccggtg aaagaataac atcttaaatt aataaaagcc
     2341 agagattttt tatcattttt taacattgca caaaaaaaac taacaaataa ttaaagttgt
     2401 atcttaaaag gaagaagcaa aagtaattat acagccgtta gatgacaaac taacttgagt
     2461 ttttagacaa aaccaaaact cataacaaaa accaccgcgc tcgtttgagt tcggcggttt
     2521 agtttttgtg cagaatcgta acttgtacaa aaacaaagac ttttgtacaa aaactctatt
     2581 ttctgcacaa aaacatctta ttttaacctg gtttttgtgc aaagccagtt gcgat
//
LOCUS       DASWNE010000139         3074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_286882, whole genome shotgun sequence.
ACCESSION   DASWNE010000139 DASWNE010000000
VERSION     DASWNE010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286882"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1323)
                     /locus_tag="VGB68_02895"
     CDS             complement(<1..1323)
                     /locus_tag="VGB68_02895"
                     /inference="COORDINATES: protein motif:HMM:NF014675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HEY0048206.1"
                     /translation="MKKQRLVEIKFYAAFAVLLMLFAMAWTPTAVKAQTRAEYRAFWV
                     DTFNTSLNNHNDVVTVVNNAKAAKANALFVQVRRRGDSWYLNSLEPMGDRTPIQPGFD
                     PLQDIINEAHANDIEVHAFVIMSAVWGRAPNLFPPENPNHVFNRHGGFNPATNTIAPG
                     PNNWLTRTLIPDGTAAITYQGHRFGSDFWVDFGHPDAAAYTVNVINHLVQNYDIDGLH
                     LDRIRYPEIGIAGQTPSTGANVGYNSTSVERFQRYYNIPPGTPPPAPNNPQWSQWRRD
                     QVTNIVRRVYLNALAVKPNLKISAALIAFGGIGSTEASWNSAEAYWRVYQDWRAWTQE
                     GIIDIAIPMVYKAEHTASVRPQYDQWDAWLRGHLYNRAGMMGQGAANNAVEGTLRQTR
                     RTLLPPAGVPNNLSGIIYFSMATSNIAVTNNPFAVPSPITTPARPFAEF"
     gene            1649..2257
                     /locus_tag="VGB68_02900"
     CDS             1649..2257
                     /locus_tag="VGB68_02900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0048207.1"
                     /translation="MSETVLKKTGTEFSAKGLAKTDAELLQACRKGDEAAWNDLVDRY
                     QRLISTIPRRAGLSEEQTADVFQEVFLTLFEKLDEIEQPDKLRSWLVTTAKFKTWRVV
                     RGEKGFYSPETEEEMAEEMASLPDSSPLADAVLIRMEEQHQIRTALKGLEERCRKILS
                     MIYLRDTAASYAEVADAIGVGETSISPMRSRCLQKLAKILTK"
     gene            2437..2931
                     /locus_tag="VGB68_02905"
     CDS             2437..2931
                     /locus_tag="VGB68_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048208.1"
                     /translation="MILAMSNDSNDELINKIIHLMQTDDSADAPADSVKWAKNIFRTR
                     ASEPKKSLVQKILAVLQMDLSGAQAAFGERSATLSQTRQMLFQAGENAVDLRISEDEN
                     GFEVQGQILGEGFSNCIVRLGDFQAKANELSEFKFTVVPGGKYDLVLQSADREIEIAG
                     LELN"
BASE COUNT          744 a          767 c          853 g          710 t
ORIGIN      
        1 aaattcggcg aacgggcgcg ccggcgtggt aatcggcgac ggaacggcaa acggattatt
       61 ggtcacggca atgttgctcg tcgccatcga aaagtaaata attccgctca gattgttcgg
      121 aacgccggcg ggcggcaaaa gcgtccgccg cgtttgccgg agcgtgcctt cgacggcgtt
      181 gttcgccgcg ccctgaccca tcattccggc gcgattataa agatgtcctc tgagccacgc
      241 gtcccactgg tcgtattgcg gtcggacgga agccgtgtgt tccgccttgt aaaccatcgg
      301 aatcgcgatg tcgataatgc cttcctgcgt ccacgctcgc caatcctgat aaacgcgcca
      361 gtaggcttcc gccgaattcc acgaagcttc cgtcgagccg atgccgccga aggcgataag
      421 cgcggcggaa attttcaaat tcggtttgac ggcaagcgcg ttcagataaa cgcggcgcac
      481 gatattggtc acctggtcgc gccgccactg gctccattgc ggattattcg gcgcgggcgg
      541 cggagtgccg ggcgggatat tgtagtaacg ctgaaaacgc tctacgctcg tcgagttata
      601 gccgacattg gctccggtcg agggcgtctg accggcgatg ccgatttcag gatagcggat
      661 gcggtcgaga tgcagcccgt cgatgtcata gttttgaacc agatgattga tgacgttaac
      721 cgtatacgcg gcggcgtcgg gatgtccgaa atcgacccag aaatcgctgc cgaaacggtg
      781 tccctgataa gtgatagccg ccgttccgtc cggaatcagc gtgcgcgtca gccagttgtt
      841 cggtcccggc gcaatcgtgt tcgtcgccgg attgaagccg ccgtgccgat tgaaaacgtg
      901 gttcggattt tcgggcggaa aaagattcgg cgcgcgtccc cagacggcgc tcataatgac
      961 gaaagcgtga acttcgatgt cgttggcgtg cgcctcgttg ataatatcct gcaacgggtc
     1021 gaagccgggc tgaatcggtg tccggtcgcc catcggttcg agcgaattca gataccacga
     1081 gtcgcctcgc cgccgcacct gtacgaacag ggcgttggct ttcgccgctt tcgcgttgtt
     1141 gacgaccgtg acgacgtcgt tatgattatt gagcgaagtg ttgaaagtat cgacccagaa
     1201 ggcgcgatac tcggctctgg tctgggcttt gactgccgtc ggcgtccaag ccattgcgaa
     1261 cagcattaaa agaacggcga acgccgcgta aaatttaatt tcgacgagtc tttgtttttt
     1321 cataatttct tccttcctgc gaacagtcct ttatcggctc tctcgccgcg cgccaaagaa
     1381 taaacgggaa cgggtcgata cgacatttta ggaaatcgga gagcctgaat cagcgtgctc
     1441 ttaaataaac agtcaattgt ttgtaaagca gatacgcatt attgtcactt atatataatt
     1501 tgattgtcaa taaaatttct tatatgagtt ttaataaaat tcttcagatt ccggccgccg
     1561 ccgagccttc tctgaaaaca gcggcaggag acaaatttac cgtcacctgt tgattatttt
     1621 tttatcctat ttcaaaatca gaacttaagt gagcgagacg gttctaaaaa aaacggggac
     1681 ggaattttcg gcgaaaggtc tggcaaaaac ggatgccgag cttttgcagg cgtgccggaa
     1741 aggcgacgaa gccgcctgga acgatttggt tgaccgctac cagcgcttga tttcgacgat
     1801 tccgcgccgc gccgggcttt ccgaagaaca gacggctgac gtttttcagg aagtttttct
     1861 gacgctcttt gaaaagctcg acgaaatcga gcagcccgac aaactccgct cgtggctggt
     1921 gacgaccgcc aaatttaaaa cgtggcgcgt cgtgcgcggc gaaaagggtt tttattcgcc
     1981 ggaaacggaa gaggaaatgg ctgaagaaat ggcgagcctg cccgattcgt cgccgcttgc
     2041 cgacgcggtt ttgattcgga tggaagaaca gcaccagatt cgcacggcgc tcaaaggact
     2101 cgaagaacgc tgccggaaaa ttctttcgat gatttacctg cgcgacacgg cggcgagcta
     2161 cgccgaagtt gccgacgcca tcggcgtcgg cgaaaccagc atcagcccga tgcgttccag
     2221 atgcctgcag aaactggcga aaattcttac aaagtagttg cagaaagctg cagaaagttg
     2281 caggcagttg cagatagttt taagctcaag cgtttacaaa gtaatttaac tttctgcaac
     2341 tttctgcaac tgcctgaaaa ctacctgaaa acttttttca acggcgtgtc tttttacttc
     2401 ccgacgtcgc actgttaagt tgtgaggttg agaaaaatga ttttagcaat gagcaacgat
     2461 tcaaacgacg aactaatcaa caagattatt cacctgatgc agacggacga ttcggctgac
     2521 gcacccgccg attcggtcaa atgggcgaaa aacatcttcc gcacacgcgc ttcggagccg
     2581 aaaaaatctc tggtgcagaa aattctcgcg gttttgcaga tggatttatc aggcgcgcag
     2641 gcagccttcg gcgagcgctc cgccactctc tcacaaacgc gccagatgct ttttcaggcg
     2701 ggcgagaacg ccgtcgacct gagaatctcg gaagacgaaa acggctttga ggtgcaggga
     2761 cagattttgg gcgaaggctt ctcgaactgt attgtcaggc tcggcgattt tcaggcgaaa
     2821 gccaatgagc tgagcgaatt caaatttact gtcgttccgg gcggaaaata cgatttggtt
     2881 ttgcagtcgg cggacagaga aatcgaaatc gccggtttgg aattaaatta agaccggctt
     2941 ttgttgaatt aagacgagct tcaagaggag aatagggaaa ccggaaggcg aaaagttgca
     3001 ggacttttcg ccatttttat ttaaacgccg cgagttcggt ttaaggaaaa ttcgctgccg
     3061 gcggcgagaa aacg
//
LOCUS       DASWNE010000140         2542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_39812, whole genome shotgun sequence.
ACCESSION   DASWNE010000140 DASWNE010000000
VERSION     DASWNE010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39812"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(346..>2542)
                     /locus_tag="VGB68_02910"
     CDS             complement(346..>2542)
                     /locus_tag="VGB68_02910"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEY0048209.1"
                     /translation="DELGFEVISGVNQNKAQMKKLIREFGDKLMIQKGVGLFFFAGHG
                     VRSGNSNYLVPVDAEILREDEIEDQAVDINLVMGKMDSAQTNLNIIMLDACRNNPFAK
                     EWSKYRAVNEQDGLAKIDAPRGTVMFYAAEPGKVAWDGLGRNGTFTEALLEQIRKPDV
                     ELDAMIKLVKRAVLEKSKNKQAAYVEATNFNDFYFAKTADRNKPPAPEKVSKSAGADD
                     SNIKEKDAATLEQEAWDLTKNSTDEDIFRLFLKEFPNGANAAKAKIKLEQMVWDSIKT
                     TSDPDAVERFLQEFPDGANKSAARIKLQRLKTREKITADEKITADEKTPTEPKTSSES
                     KTSGETEKLSQPKTPVESKTSSEPDASNEPRTSSEPKKVNEPVGKQTESAPNVDGKSR
                     FQREAEAWDAVRSSTNGRELRAFLEIFPDGVNAEKAKIRLEQVLWDARRVTKDKALIE
                     EQLKEFPKGANAPLARIMLRQLEKPETGKPADGDAKGWSNALGMELVFIPAGSFMMGA
                     SAANISESLRVARKDYADIDQTQLNNEKPTRRVTFAKGFWMGKFEVTQAQWAAVMGDN
                     PSNFKGCDNCPIERVSWEMAKIFVQRLNELDDGLVYRLPSEAEWEYAARAGTTGVFSG
                     RIEAMSWHSGNSEDKTHPVGEKLPNPFGLYDIYGNVSEWCEDIYSPTYEGLPVDGSAN
                     TSVGNNRMRVIRGGSWNNFPTKSRSTFREPYPANAFGMAIGFRVAAVRK"
BASE COUNT          507 a          718 c          565 g          752 t
ORIGIN      
        1 acttagcact gattaaattc tattttcgat agtttgtcaa catttttata atcattcgtt
       61 gagcaacaca aatttacaac actacctgaa ttcttttaaa tatccttcaa aatccgttga
      121 acattttggg aatcttcaat ctttcggaaa atgaagagaa ttcatcgctt tacgcgtcgg
      181 catcaaacac gaaaatctac gcgccgaatt gatcaaggga aaactcggag agtaagtaaa
      241 aaacgcaaag tgcaaaatat caggatttag aattcaggat taaattcttt ttccgttcag
      301 cactttgcac tttgcgtttt ttacatattt tgcatttgtt aagcgttatt tccgaaccgc
      361 cgcgacgcgg aagccgattg ccataccaaa ggcattggcg ggatacggct cccggaaggt
      421 cgagcgcgat ttggtcggaa aattgttcca cgacccgccg cgtataaccc gcatcctgtt
      481 gtttccgacg ctcgtgttcg ccgagccgtc aacgggcaag ccttcatacg tcgggctgta
      541 aatatcctcg caccattccg agacgttgcc gtaaatgtcg tagagcccga aaggattcgg
      601 cagcttttcg ccgaccggat gcgtcttgtc ttccgagttt ccgctgtgcc aggacatcgc
      661 ttcgatgcgt cccgaaaaaa ctccggtggt gcccgcccgc gccgcgtatt cccattcggc
      721 ttcgctcggc aggcgataca ccagaccgtc gtccagctca tttagtctct gcacaaagat
      781 tttcgccatt tcccagctga cgcgctcaat cggacagtta tcgcagcctt tgaaattgct
      841 cgggttatcg cccatcaccg ccgcccattg cgcctgcgtc acttcaaatt tccccatcca
      901 gaaccctttg gcaaacgtca cgcggcgcgt cggcttttca ttattgagct gagtttggtc
      961 aatatcggcg taatctttac gagcgacgcg cagggattcg gaaatattcg ccgcgctggc
     1021 gcccatcata aaactgcccg ccggaataaa gaccagctcc atccccagcg cattactcca
     1081 gccttttgcg tcgccgtcag ccggcttgcc cgtttccggt ttttccaact gccgcagcat
     1141 tattcgcgcc agcggagcgt tcgctccttt cggaaattct tttagctgtt cttcaatcag
     1201 agccttatct ttcgtgactc ttctcgcgtc ccagagaacc tgttccagcc ttattttagc
     1261 tttctccgcg tttacgccgt cgggaaaaat ttcgagaaaa gcgcgtaatt cccgcccgtt
     1321 tgtgctgctt ctgaccgcgt cccaggcttc cgcctcgcgt tggaatcggg attttccatc
     1381 gacgttcggc gcgctttcgg tctgcttgcc gacaggctcg ttaacctttt taggctcgct
     1441 cgacgttctc ggctcgtttg aagcatccgg ttcgctcgac gtttttgatt cgaccggcgt
     1501 tttcggctga cttaactttt ccgtttcgcc tgatgttttt gattcgctcg acgttttcgg
     1561 ctcagtcggc gttttttcgt ccgctgtaat tttttcgtcc gctgtaattt tttctctcgt
     1621 tttgagtcgt tgaagcttta tgcgcgccgc gcttttattc gcgccgtccg gaaattcctg
     1681 caaaaatcgc tcgaccgcgt ccgggtcact cgtcgttttt atcgaatccc agaccatttg
     1741 ctcaagcttt attttggctt tcgccgcatt cgcgccgttc ggaaactctt tcagaaaaag
     1801 gcgaaaaata tcctcgtccg tactgttttt cgttaagtcc caggcttcct gttcaagcgt
     1861 cgcggcatct ttttccttaa tatttgaatc gtccgctccg gcggatttgg agaccttttc
     1921 cggcgcgggc ggcttattcc tgtccgccgt tttcgcaaaa taaaaatcgt tgaaattcgt
     1981 cgcctcgacg taggcggctt gcttgttttt cgatttttcc agaacagcgc gttttaccag
     2041 tttaatcata gcgtcgagtt cgacgtcggg tttccttatc tgttccagca gcgcttcggt
     2101 aaaagttccg tttctgccga gcccgtccca ggcgactttg cccggttccg ctgcgtaaaa
     2161 catcaccgtc cctctcggag cgtcgatttt cgccaagccg tcctgctcat tcaccgcgcg
     2221 atatttgctc cattccttag cgaacggatt gttgcggcac gcatccagca taatgatatt
     2281 cagatttgtt tgcgccgagt ccatttttcc catcaccaaa ttaatgtcta cggcttggtc
     2341 ttcgatttca tcttcgcgaa gaatttccgc gtcgaccgga acgagataat tactgttgcc
     2401 tgagcgcacg ccgtgtccgg caaaaaagaa aagccccacg cctttttgta tcatcagctt
     2461 gtcgccgaat tcccggatga gttttttcat ctgcgccttg ttttgattga cgcccgatat
     2521 tacctcaaaa cccagctcat cg
//
LOCUS       DASWNE010000141         5511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_39841, whole genome shotgun sequence.
ACCESSION   DASWNE010000141 DASWNE010000000
VERSION     DASWNE010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5511
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39841"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(234..989)
                     /gene="fabG"
                     /locus_tag="VGB68_02915"
     CDS             complement(234..989)
                     /gene="fabG"
                     /locus_tag="VGB68_02915"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEY0048210.1"
                     /translation="MSEKQFSGKSAIVTGATRGIGKAIALELARRGANVAFNYARSAD
                     EAEKMKAEIEGLGVKAFAAQCDVANTEASAGFVKQVQTEFGTIDFLVNNAGITRDTLI
                     LRMKEEDWDSVIDTNLKGAWNFSKAVLRPMMRNDAGGSILNISSVSGVVGMQGQSNYS
                     ASKAGMIGLTKALAKEVASRKITVNALALGLIETDMSGEMNEEHRTKILEMIPLGRLG
                     NVREVAEIACFMLSDAARYITGQVIQPDGGLAI"
     gene            complement(1005..1709)
                     /locus_tag="VGB68_02920"
     CDS             complement(1005..1709)
                     /locus_tag="VGB68_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048211.1"
                     /translation="MGLDLVEMVLRVEDTFGVEIPDEAAATLETPRLVIDFLMTRLDT
                     RAPRTCISQEAFYFLRRKVLAQTPFPRERFHPRTTLEEVFPKKNRRELWRNLSSEMGS
                     KSALPGLVRPDWLRLSIAFICLGIFILIAAGVNYSASHITATFVIGLPAIFGAAALLI
                     YLTQSFQNQFALDYQKIGDLVEYSISHHVCAFKTEGWTREQIAGIVWNIIEEETGVDV
                     SKFNEDSYFIRDMNLD"
     gene            complement(1716..2021)
                     /locus_tag="VGB68_02925"
     CDS             complement(1716..2021)
                     /locus_tag="VGB68_02925"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HEY0048212.1"
                     /translation="MALGGRSWDEWIAEYSKGHQNKINQITHKIGIPMIALSILLIIP
                     SFFIAGFWMIPLALFVLGWILQFVGHAFEGKPPEFFKDPRFLMVGLRWWLAKLRGKA"
     gene            complement(2125..3000)
                     /gene="galU"
                     /locus_tag="VGB68_02930"
     CDS             complement(2125..3000)
                     /gene="galU"
                     /locus_tag="VGB68_02930"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEY0048213.1"
                     /translation="MTQKVRKAVFPAAGLGTRFLPATKASPKEMLPLVDKPLIQYSVE
                     EAVNCGIESILIITGRDKTAIEDHFDISFELEQVLKERGKDEMFDLVRAVSELARISY
                     MRQKQALGLGHAIYQAKDFVGNEPFAVLLADDVVDAEKPALQQLIDVYEKYNAPVIAT
                     MQVEGEAISRFGVIDAKEEVEPGVFKVREVIEKPKFEDAPSDMAVIGRYIFTPELFDA
                     LEHTKPGAGGEIQVADAMRELLNDGFPFYAVRLEGTRHDAGDKLGFLIATVEFALKHE
                     DLAADFRKYLKTLNL"
     gene            3174..4223
                     /locus_tag="VGB68_02935"
     CDS             3174..4223
                     /locus_tag="VGB68_02935"
                     /inference="COORDINATES: protein motif:HMM:NF018880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048214.1"
                     /translation="MNKIFFLTILTILSVNAFVIQAQIAPCAQTKAVTLPLAGEYGSF
                     GQFAVTRQSIPNPDPNAPAPISVFVPANATAQTRVPVVFFAHGFGGTSFQFYETLLRQ
                     LSSYGYIVVFSPYTANLFAAHATRYNQLWNGFAAAVQQYGDIMDTTRAGFAGHSYGAG
                     ATPEMARRGAAQGWGANGLFLFIMAAWYNWGTNPEQIPSSAKMIVQVYWDDATNQHLI
                     SQNDIWNKLPQITERKWQVIRASRTFCALDAGHSVPVTDGLGQTEATTDAYDGWGVWR
                     RLHALSDYTFSGNQTAKTIAFGIDTNMGRWRGIFGVRRISPLEATDAPVVNSQSNPTF
                     LWSQKCSYALGSPCP"
     gene            4332..>5511
                     /gene="serS"
                     /locus_tag="VGB68_02940"
     CDS             4332..>5511
                     /gene="serS"
                     /locus_tag="VGB68_02940"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEY0048215.1"
                     /translation="MLDLNFVRENLETVREVLNNRHFPSKSLEKFVELDAERRFVIGE
                     SDRLNAQRNSASKEIGALMQAGNLAEAEAKKAEVAGLKEKQSELERRRDESENAMRDL
                     LAALPNIPADDVPVGADESANVEVRRWGAPREFDFEPKDHVDLGESLDILDLERATKI
                     AGSRFAILNGAGARLERALVNFMLDVHTTEHGYTETLPPFIVNRQALFGTNQLPKFEA
                     DLFHLTDERGFALIPTAEVPVTNYHAEEILEAKDLPKYYTAYTPCFRSEAGSYGRDTR
                     GLIRQHQFEKVELVKLSLPENSVEEHEKLTANAERILQLLGLPYRTVVLSTGDMGFGA
                     MKTYDIEVWLPSQKTYREISSCSNCGDFQARRMNLRFRRAGGAKPEFVHTLNGSGLAV
                     G"
BASE COUNT         1357 a         1485 c         1325 g         1344 t
ORIGIN      
        1 agttttatgt aatgtaaaac tattaaaccc agcaataagg cttttaaaat ccaattttcg
       61 attctctgta aatttccgtt ttccatatcc tacctctcta ggatataaag tagatagaac
      121 ttaaaataaa cccctttaat acttgccgta gtaattgcta acacttttga ttttcgcgct
      181 agactttata ggcaaaattt agagattata ggcaaagcca cttaaaaccg aaattatatc
      241 gccagcccgc cgtccggctg gatgacctgt ccggtaatat aacgcgccgc gtcggacagc
      301 ataaagcagg cgatttccgc cacttcccgc acgtttccga gccgcccgag cggaatcatt
      361 tccagaattt tggttcgatg ttcttcgttc atttcgcccg acatatcggt ttcgattaaa
      421 ccgagcgcta gcgcgttaac cgtaattttc cggctggcga cttcctttgc cagcgctttc
      481 gtcagaccga tcattcccgc tttcgaggcg gaataattcg attgtccctg catcccgacg
      541 acgccgctga cggaagagat attcagaatc gagccgcccg catcattgcg catcatcggg
      601 cgcagaaccg ctttcgaaaa attccaggcg cctttcagat tcgtgtcgat aacgctgtcc
      661 cagtcttctt ccttcatccg cagaatcagc gtgtcgcgcg tgattccggc attatttacc
      721 aaaaaatcaa tcgtcccgaa ctcggtttgc acctgtttca caaaaccggc tgacgcttcc
      781 gtgttcgcca cgtcgcactg cgcggcaaaa gccttcacgc ccaaaccttc gatttccgct
      841 ttcatctttt cggcttcgtc cgcgcttctc gcataattaa aagcgacatt cgcgccgcgc
      901 cgcgccagtt ccagcgcaat cgccttgccg atgccgcgcg tcgcgcccgt gacgattgcc
      961 gattttccgc taaattgttt ttcgctcaat ttataactcc tgaattagtc gagattcata
     1021 tctctaataa aatatgaatc ttcattaaat tttgaaacgt cgacaccggt ttcttcttca
     1081 ataatattcc agacaatccc ggcaatttgt tcccgcgtcc aaccttccgt cttgaaagcg
     1141 caaacatgat gcgaaattga atattctacc aagtcgccga ttttttgata atcgagcgcg
     1201 aattgatttt gaaaagactg tgtcaaataa attaaaagcg ccgccgcgcc aaaaatcgcc
     1261 ggcaacccga taacgaatgt cgcagttatg tgcgaagcgg aataatttac gcccgccgcg
     1321 attagaataa agatacccaa acagataaaa gcgattgata ggcgaagcca atcgggacga
     1381 accaaaccgg gcaatgcgct tttgctgccc atctcgctac ttaaatttcg ccaaagctct
     1441 cgtcgatttt tcttcggaaa cacttcttct aaagttgtcc gcggatgaaa ccgctcgcgc
     1501 ggaaacggcg tctgcgccaa aaccttcctt cttaaaaaat aaaatgcctc ttgtgaaata
     1561 caagttcggg gcgcgcgcgt atcaagccgg gtcatcaaaa aatcaattac caggcgcggg
     1621 gtttccagag tggctgcagc ttcgtcagga atttctactc cgaaagtatc ttcaaccctc
     1681 agaaccattt caaccaaatc taatcccata aacttttatg ctttgccgcg cagtttcgcc
     1741 aaccaccagc gcagcccgac catcagaaag cgcgggtctt tgaaaaattc gggcggcttg
     1801 ccttcgaaag cgtgaccgac aaactgcaaa atccagccga gaacaaacag cgcgagcgga
     1861 atcatccaaa agcccgcgat gaaaaaactc ggtataatca gcaaaatcga aagcgcaatc
     1921 atcggaatgc cgattttatg cgttatttga ttgattttat tctgatgacc tttcgaatac
     1981 tccgctatcc attcgtccca acttcttccg ccaagcgcca taaattttca cctcgcaaag
     2041 aatttttgct gcaaaggcac gacagagact cgaagacttt tgcatttgtc gcgccatttc
     2101 tatgcctttg cggtaaaaag tttactacaa attcaaagtt ttcagatatt ttctaaaatc
     2161 tgccgcaagg tcttcgtgtt tgagcgcgaa ttcgaccgtc gcaatcaaaa atccgagctt
     2221 gtcgcccgcg tcgtgccgcg tgccttccag cctgacggcg taaaaaggaa aaccgtcgtt
     2281 gagcaattcg cgcatcgcgt cggcgacctg aatctcgccg cccgcgcccg gtttcgtgtg
     2341 ttcgagcgcg tcgaaaagct caggcgtaaa aatataacga ccgatgaccg ccatatccga
     2401 cggcgcgtct tcaaacttcg gtttttctat aacctctcgg actttgaaaa cgcccggctc
     2461 gacttcttct ttcgcgtcaa tcacgccgaa acgcgagata gcttcgcctt cgacctgcat
     2521 cgtcgcaatc acgggcgcat tgtatttttc gtaaacgtcg atcaattgct gcaaagccgg
     2581 tttttcggca tcgaccacat cgtccgccag cagaacggca aacggctcgt tgccgacgaa
     2641 atccttcgcc tgataaatcg cgtgtcccaa gccgagagcc tgtttttgcc gcatatagga
     2701 aattctcgcg agctcggaaa ccgcgcggac gaggtcgaac atttcgtctt ttcctctttc
     2761 ttttaaaacc tgttcgagct cgaatgaaat atcgaaatgg tcttcaatcg cggttttgtc
     2821 gcgacccgtt ataatcagaa tggattcgat gccgcagttg accgcttctt cgaccgaata
     2881 ttgaatcaga ggtttatcga cgagcggcag catctctttc ggcgatgctt tcgtggcggg
     2941 cagaaaacga gttcccagac cggcggcggg aaagacggct ttacgtactt tttgtgtcat
     3001 aatatgtccc ttgttcttaa attgtgtaaa acattgattc tgacatatcc ggcgaggtgc
     3061 gaacaaatgt gcgaaaaaca aacttttgaa gttttaccgg aaaaagacga aatattcgga
     3121 cgccgcgcgc atcacgattc cgctcgacgc ggaaaaaatt ttcggagttt tcaatgaata
     3181 aaatattttt cctgacaatt ttaacgattc tttccgtcaa cgctttcgtc attcaggcgc
     3241 agattgcgcc gtgcgcgcag accaaagcgg tgacattgcc gctcgccggc gaatacggca
     3301 gcttcggtca gtttgccgtc acgcggcagt cgattccgaa tcccgacccg aacgcgcccg
     3361 cgccgatttc ggtttttgtg cccgctaatg cgacggcgca aacgcgggtt ccggtcgttt
     3421 tcttcgcgca cggcttcggc ggcacgagct tccaatttta cgaaacgctg ctgcgccagc
     3481 tttccagcta cggctacatc gtcgttttct cgccttatac cgccaattta ttcgcggcgc
     3541 acgcgacgcg ctacaatcag ctctggaacg gttttgccgc cgccgtccag caatacggag
     3601 atattatgga cacgacgcgc gccgggttcg ccggtcattc ctacggcgcg ggcgcgacgc
     3661 ccgaaatggc gcggcgcggc gcggctcagg gctggggcgc aaacggttta tttttgttta
     3721 ttatggcagc ctggtataac tggggaacaa atcccgaaca aattccgtcg agcgcgaaaa
     3781 tgatcgtgca ggtttactgg gacgacgcga ccaaccagca tctgatttcg caaaacgaca
     3841 tatggaataa actgccgcag attaccgaaa gaaaatggca ggtcatccgc gcttccagaa
     3901 cattttgcgc tctggacgcc gggcattcgg ttcccgtgac cgacggcttg ggacaaaccg
     3961 aagcgacgac cgacgcctat gacggctggg gcgtctggcg gcggcttcac gcgctttcgg
     4021 attacacttt cagcggaaat cagacggcga aaacaatcgc cttcggcatt gatacgaata
     4081 tgggcagatg gcgcggaatt ttcggcgttc gccggatttc gccgctcgaa gcgaccgacg
     4141 cgcccgtcgt caattcccaa agcaacccga catttctctg gagccagaaa tgcagctacg
     4201 cgctcggctc gccctgcccc tgagcagagt gaaagattgc cttcgaaatg cggcggtctt
     4261 tgaccgtcgt tttagtttaa gttgataata ttatttcatt tgacgaatgt gaataaagga
     4321 attttataaa aatgctcgat ttgaattttg ttcgtgaaaa tctggaaaca gtccgggaag
     4381 ttctgaataa tcgccatttt ccgtctaaga gtctcgaaaa atttgttgaa ttagacgccg
     4441 aacgccgctt tgttatcggc gaatcagacc ggcttaacgc gcagagaaat tcagccagca
     4501 aggaaatcgg cgcgctgatg caagccggaa atcttgcgga agccgaagcg aaaaaagcgg
     4561 aagtcgcggg tctgaaagaa aaacaatccg agctcgaacg ccggcgcgac gaatcggaaa
     4621 acgcgatgcg cgacctgctc gccgctttgc cgaatattcc cgccgacgac gttcccgtcg
     4681 gcgcggacga atcggcaaac gtcgaagtgc gccgctgggg cgcgccgcgc gaattcgatt
     4741 ttgagccgaa agaccacgtc gatttgggcg aaagcttaga cattctggat ttggagcgcg
     4801 cgacgaaaat cgccggttcg cgctttgcga ttttaaacgg cgcgggcgct cgactggagc
     4861 gcgctctggt aaattttatg ctcgacgttc acacgaccga gcacggttat acggaaactc
     4921 tgccgccgtt tatcgtcaat cgtcaggcgc ttttcgggac gaatcaactg ccgaaattcg
     4981 aggcggattt gtttcattta acggacgaaa gaggttttgc cctgattccg acggcggaag
     5041 ttccggtgac gaattatcac gccgaagaaa ttctggaagc gaaggatttg ccgaaatatt
     5101 atacggctta cacgccctgt tttcgctccg aagccggttc ttacgggcgc gatacgcgcg
     5161 ggctgattcg ccagcatcaa tttgaaaaag tcgagctggt gaaattgtct ctgccggaaa
     5221 attccgtcga agaacacgaa aagctgacgg caaacgccga acgcattttg cagcttctcg
     5281 gcttgccgta ccggacggtc gttttatcga ccggcgatat gggcttcggc gcgatgaaaa
     5341 cctacgacat cgaagtctgg ctgccttcgc aaaaaactta tcgcgaaatt tccagctgct
     5401 cgaactgcgg cgattttcag gcgcggcgaa tgaacctcag attccggcgc gcgggcggcg
     5461 caaaaccgga attcgttcac actttgaacg gctcgggact ggcggtcggc a
//
LOCUS       DASWNE010000142         4377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_356952, whole genome shotgun sequence.
ACCESSION   DASWNE010000142 DASWNE010000000
VERSION     DASWNE010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356952"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1751
                     /gene="lnt"
                     /locus_tag="VGB68_02945"
     CDS             165..1751
                     /gene="lnt"
                     /locus_tag="VGB68_02945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEY0048216.1"
                     /translation="MKFFTAAKAKFPSLQNSLLAVLSAILLVLAFPGFELWLLAWFAF
                     VPLFWAIEREKHSMVKSFLLGWLWGSVFFFGSCWWLAYAPTHYGGMPALVSYFLVLCA
                     CAAIGIFPAIFALVFSVLLRRLGVYAVFSAPFLWTAIEFLRFWTTGNNWNAVGYSQAF
                     RLSILQFASVGGIYLVGFMVVSTSSYVAWKLLFLLSEKKTKYFYLTILLYVSLLAASF
                     ELAGFPKRSEALPGEMQTYVVAIQPNVPMSGLKYEDWRRLRGRHVELTENALRNIRRA
                     SAEQPVVVIFPESPMNFQYQRDREFQEFLSVLTRRNNVSILFNSAEPDAARDSYFNSA
                     VMVNEKGEKIAQYDKIFLLPFGEYAPVPAPLQSLVPTMVGNFSFGKEYDLLAFGAARG
                     GVMICFESHFPNLAREYARAGADVLIEMTNDGYLGNTPVLRQHLASAVFRAVETRRPV
                     LRVTNVGVTAYINERGEISDAAEPYTEASRVWTIAKSDGKQTFYVKYGDWFAWLCALF
                     GIVLILSGLRRKLAKDENKA"
     gene            1962..3078
                     /gene="prfB"
                     /locus_tag="VGB68_02950"
     CDS             join(1962..2024,2026..3078)
                     /gene="prfB"
                     /locus_tag="VGB68_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_319006089.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEY0048217.1"
                     /translation="MIELTDLQTKYEEIKSQVEQLGGFFDAPKKRAELEKLEAEISAP
                     DFWNDSEKAQKTVQRRSRLERAIARQEAFETGVSDAEVLFEFAETDESSLQELNDLIE
                     RLEREAGDAEIEVLLSGQTDANNAICSIQSGAGGTDAQDWAQMLLRMYLRWAEKRGFK
                     VDILDEQAGGEAGIKSATFRVEGEYAYGLLSCEAGVHRLVRISPFNSGGSRETSFASL
                     FVSPEIDENIEVDIQDKDLRVDTYRSTGAGGQHVNTTDSAVRITHLPTGTVVTCQNQR
                     SQIQNRAVAMQVLRSKLYELELAKRREGVAELEASKQDIAFGSQIRNYTLQPYRLVKD
                     ARTKFERNDVDTVLDGDIDDFIKAYLLFRKSSQQAIN"
     gene            3078..>4377
                     /locus_tag="VGB68_02955"
     CDS             3078..>4377
                     /locus_tag="VGB68_02955"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEY0048218.1"
                     /translation="MATKVQKKQAAKSYSMTSEIIASLLLIIAVLVFLSLISFDVHDR
                     YCCNSSTTQVTQNWIGVIGANIAGILINETVGLTAYVIPALLALIAWRFFRSENVLPR
                     PSRIIGFIIFVASLSGLFELFGGEGALVGETVRRVFVYLLGAVGATIVLGGLFFTSLL
                     LITNLSYFSFLSGFKTIFDFLGTSEWSARFSAWREEQNRIAQERLERRREMRVERENP
                     TISAGETPIAKNGKTAVTAETVSPLGAKIGDIFKRTSEKPKEVEPKIEPKIEPKIEPK
                     VEERVEAVEETFEEEEIRPTISTSEENNFTGVEVSPDDETDDLVLPKHLDEEIAQIPI
                     SPMKATGELENVLQEEDVEIDEIDEKPFVNEKLPPQNFDNYVLPEPQFLTAAAPHVEQ
                     KEESLLAVAQELTEKTKEFNVTGRVMHICQGPVVTTYEFKP"
BASE COUNT         1041 a         1068 c         1194 g         1074 t
ORIGIN      
        1 gtgttgaata atcagcgcgg gcttttcttt cggttgtgta gaaaaaagcg cgaatcttat
       61 gatttcaatc aaagaatcgg tatcaaatat cagttcgtca ccggctttcc gcccgacatt
      121 ttctccgtat tctctgtaac ggaatttttt gctaaactgt cggcatgaag tttttcacgg
      181 cggcgaaagc gaaatttccc tcactgcaaa attcgctttt agccgtttta tcggcgattc
      241 tgctggttct ggcttttccc ggtttcgagc tctggctgct ggcttggttt gccttcgtgc
      301 ctttgttctg ggcaatcgag cgcgaaaaac attcaatggt caaatcattt ctgctcggct
      361 ggctttgggg aagcgttttc tttttcggca gctgctggtg gctcgcctac gcgccgacgc
      421 attacggcgg aatgcccgcc cttgtgtctt actttctggt tttatgcgcc tgcgcggcga
      481 tcggaatttt tccggcgatt ttcgcgctcg tgttttcggt tctgctcagg cggctcggcg
      541 tctacgcggt tttttcggcg ccgtttttgt ggacggcgat tgagtttttg cggttctgga
      601 cgaccggcaa taattggaac gccgtcggct attcgcaggc gtttagatta tcgattctcc
      661 agtttgcttc ggtcggcggg atttatctgg tcgggtttat ggtcgtctcg acgagttcct
      721 acgtcgcctg gaaattatta tttcttttgt cggaaaaaaa aacgaagtat ttttacctga
      781 cgattttgct ttacgtttcg cttctggcag cttctttcga gctggctgga tttccaaaac
      841 gctcggaagc tttgcccggc gaaatgcaaa cttacgtcgt cgccatccag ccgaacgtgc
      901 cgatgagcgg cttgaaatac gaagactggc gaaggttaag aggaaggcac gtcgagctga
      961 ccgaaaacgc cctccggaat attcggcgag cgagcgccga acaaccggta gtcgtaatct
     1021 ttcccgaatc gccgatgaat tttcaatacc agcgcgaccg tgaatttcag gaatttttaa
     1081 gcgtcctgac caggcgcaat aacgttagca ttctattcaa ttccgccgag ccggacgccg
     1141 cgcgcgacag ctatttcaac tccgccgtga tggtcaatga aaagggcgaa aaaatcgccc
     1201 agtatgataa gatttttctg ctcccgttcg gcgaatacgc gcccgttccc gcgcccttgc
     1261 aatcgctggt gccgacgatg gtcggaaact tttctttcgg caaagaatat gatttgctgg
     1321 cgttcggcgc ggcgcgcggc ggcgtgatga tttgtttcga atcgcatttt ccgaatctcg
     1381 cgcgcgaata cgcgcgggct ggcgctgacg ttttgattga aatgacgaac gacggttatc
     1441 tcggaaatac gccggttttg cgccagcatc tggcaagcgc ggtttttcgc gcggtcgaga
     1501 cgcgccgtcc ggttttgcgc gtcaccaacg tgggcgtcac ggcttacatc aacgagcgcg
     1561 gcgaaatctc ggacgcggcg gagccttata cggaagcgag tcgcgtctgg acaatcgcca
     1621 aatcggacgg aaaacagact ttttacgtaa aatacggcga ttggttcgcg tggctctgcg
     1681 cgctgttcgg tattgtttta attttgtcgg gcttgcgccg taaattagcc aaagatgaaa
     1741 ataaagcata aaattttcgt attagttttc gcgctctttt cgttttatac ttaaagctat
     1801 atcggctggc gcgaaacgcg cgcggaaatt tgggaaaaag acgaaaagcc ttacgttatt
     1861 tttccggctg acaagatttt tctatattat tttttccgtc ctctgagcta tatcgacagc
     1921 aagctcaccg gcatcggatt tcatatcgga caacaccaat aatgattgaa ttaactgatt
     1981 tacaaaccaa atacgaagaa ataaaatcgc aggtcgagca gctcgggagg tttctttgac
     2041 gcgccgaaaa aacgcgccga gcttgaaaaa ttagaagccg aaatctccgc tcccgatttc
     2101 tggaacgact cggaaaaagc gcagaaaacg gttcagcggc gaagccgtct ggaacgcgcc
     2161 atcgcgcgtc aggaagcttt tgaaacgggc gtttccgacg cggaagttct ttttgaattt
     2221 gccgaaacgg acgaaagctc tctgcaagag ctgaacgatt taatcgagcg gctcgaacgc
     2281 gaagccggcg acgcggaaat cgaggttctt ttgtcgggac aaaccgacgc gaacaacgcg
     2341 atttgctcga ttcaatcggg cgcgggcgga accgacgcgc aggattgggc gcagatgctg
     2401 ctgcggatgt atctgcgctg ggcggaaaag cgcggcttta aagtcgatat tctggacgag
     2461 caggcgggcg gcgaagcagg cataaaatcc gccacgtttc gggtcgaggg cgaatacgct
     2521 tacggcttgc tttcgtgcga ggcgggcgtt catcgcctgg tgcggatttc gccgtttaat
     2581 tcgggcggca gccgcgagac cagtttcgct tcgcttttcg tttcgccgga aattgacgaa
     2641 aatatcgagg tcgacatcca ggataaggat ttgcgcgtcg atacgtatcg ctcgaccggc
     2701 gcgggcgggc agcacgtcaa cacgaccgac agcgcggtgc gcatcacgca tcttccgacc
     2761 ggaaccgtcg tcacctgcca gaatcagcgc tcgcagattc agaatcgcgc ggtggcgatg
     2821 caggttctgc gctcgaagct ttacgagctg gagctcgcca aaaggcgtga aggcgtcgcc
     2881 gagctcgaag cgagcaagca ggacattgcc ttcggctcgc agattcggaa ttacactctg
     2941 cagccgtatc gactggtgaa ggacgcgcgc acgaagtttg agcgaaacga cgtcgatacg
     3001 gttttagacg gcgacatcga cgattttatc aaagcgtatt tgttgtttag aaaaagcagt
     3061 cagcaagcaa taaattaatg gcaactaaag tccagaaaaa acaagccgct aaaagctatt
     3121 cgatgacgag cgagattatc gccagccttc ttttgataat tgccgttctc gtgtttttgt
     3181 ctttaatctc gttcgacgtg cacgacaggt attgctgcaa cagcagcacg acgcaggtta
     3241 cacagaattg gattggcgtt atcggcgcga atatcgccgg aattttaatc aacgagacgg
     3301 tcggtctgac ggcttacgta atacccgctt tactggcttt aatcgcgtgg agattttttc
     3361 gcagcgaaaa tgttctgccg cgcccgagcc ggataatcgg atttataatt tttgttgctt
     3421 cattgtccgg cttgtttgag ttatttggcg gcgaaggcgc gctggtcgga gaaaccgtgc
     3481 ggcgagtttt cgtttacctg ctcggcgcgg tcggcgcgac gattgtcctt ggaggtttgt
     3541 tttttacttc cttgctgctt atcacgaatc tttcgtattt ttcattttta agcggattta
     3601 aaacgatttt cgattttctc ggaaccagcg aatggtcggc tcgctttagc gcctggcgcg
     3661 aggagcaaaa tagaatcgcg caggagagat tggagcgccg gcgcgaaatg cgcgtcgaaa
     3721 gagaaaaccc gacgatttcg gctggcgaga cgccaatcgc gaaaaacggt aaaactgccg
     3781 tgaccgcaga gaccgtatct ccgctcggcg caaaaatcgg cgacattttt aaacgcacaa
     3841 gcgaaaagcc gaaagaagtc gagccaaaaa tagaaccgaa aatagaaccg aaaatagaac
     3901 cgaaagtcga agaaagagtt gaagcggtcg aagaaacgtt tgaagaagaa gaaatccgcc
     3961 cgacgatttc gaccagcgag gaaaataatt ttacgggcgt ggaagtctcg cccgacgacg
     4021 agaccgacga tttggtcttg ccgaaacatt tagacgaaga aatcgcgcaa atcccgattt
     4081 cgccgatgaa ggcgaccggc gagctggaaa acgttttaca ggaagaagac gtcgaaatcg
     4141 atgaaattga cgaaaagcct tttgtcaacg agaagctgcc gccgcagaat ttcgacaact
     4201 acgttctgcc cgaaccgcag tttttaaccg ccgccgcgcc gcacgtcgag caaaaagaag
     4261 aatccctgct cgccgtcgcg caggaattga ccgaaaaaac caaagaattt aacgtaacgg
     4321 ggcgcgtgat gcacatctgc cagggaccgg tcgtgacgac ttacgaattc aagcccg
//
LOCUS       DASWNE010000143         4524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_605133, whole genome shotgun sequence.
ACCESSION   DASWNE010000143 DASWNE010000000
VERSION     DASWNE010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605133"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..3906
                     /locus_tag="VGB68_02960"
     CDS             103..3906
                     /locus_tag="VGB68_02960"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY0048219.1"
                     /translation="MTKKLISVLLAGCFCLFVFAGATFAQGTTSRVTGTVTDSSGAAV
                     SGATVTVTNEATGVALTTETSENGTYIFDLIQVGSYTLTVEKAGFKKLVSTKNTVNIN
                     QPATINAALEIGDVSATVNVESTVEQVQTSTSGNIGSTIEQRTLESLPIVGTRGRNPL
                     DLLNFQPGVVFGGNTGGAVNVHGSRDRAFNFTLDGIDINDSSAGGSNFTPLRPNPDSV
                     QEFQIITSNFTAELGRSSGAQVTLVTRSGTNRFSGNLFEYYQTPDFNAKSYPITIAGL
                     AKEQFVQHIFGGSLGGPLFNPGFGEGTKAGWLRDKAFFFVNLQMLRAYDTALVTRTVY
                     TQQARQGIFRYVQGRETINGQVVTYQNAPAGASNASVDANGNPIRPVCGGSITVNCIN
                     TYNIATTAPISIDPTLAGFINAMPLPNNFTTGDGLNTAGFNFASPQRERQYDFVTKLD
                     FKVSDTNMFYIRYAQGEQSSFGDSGNGGRPIFPDSPNLVDTIRTPKNLAVNWRFSPTA
                     RFTNEFVFGISKFAFSFDTPEPDPSIPFTFLTIATPNTNFSYNARRLRTLQFVDNLTF
                     DFSPHVIKAGVNFRFGRHIDDRSTVSGTNVEPVVTFSGAAGYNGFNLPTVSSATNPTG
                     INTNDLTLLQNTVNNLLGRIGSISQAFVSDPSNPGAFAPAGTRWINKAFYPELDFYVQ
                     DNWRFRQNLVFDIGLRWEAKLNPSVDGRPILVPNQPVKLGAAPTNSLRWVEGDLFKND
                     FGKFLPSLGFAWDPFKTGKTSIRANYRISSDRIASFLFGSSIFQSTPGNNVGATNSAF
                     GQGGGLYRNLGTVLSTLAPTSSPDILRQPPAFGTGSINVIDPDLKYPMVHSWSLSFQR
                     ELWKNNVLEVNYIGKKGTHLLGGYNANQVNIFAAAAGENFLEAFNKIREDIRINGTTN
                     ITYNSPLINALMTGSPTDNDGTARFRALVTTNTITQGNVASAALTVSQRTCQAPDVTA
                     GVCTNAQLGRRLVELGGFTYLLQPFPQFTGGLNVFDSSDYSFYNALEIIFKRRIAGGL
                     GFQLAYTLSKSMDNRSWDPSLSTVTTGSVQSASSTPFDLRDRRLNYSWSDFDRRHVFQ
                     GTYVYELPFGKDRWLSSGNKAVDYVFGGWQLSGTIIWMSGRPFTVYSGVNTVSNVVQS
                     TADCSGCTRNSGQLILENGRNFWFDAATRAMFSAPAPGSVGNTGRNFFLAPRYFQTDA
                     SLSKKFRFTERMSFDVRVDARNLTNNPSFDNPTALITSNIFGRINDSVTNNARRIQLS
                     GKFNF"
     gene            4322..>4524
                     /locus_tag="VGB68_02965"
     CDS             4322..>4524
                     /locus_tag="VGB68_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048220.1"
                     /translation="MILKKELTQSRSNFVFDNINQTARRAFGLFVVLLAGMIIFSSVV
                     ETAEAATFTVTKTGMTNDGVCNA"
BASE COUNT         1091 a         1166 c         1166 g         1101 t
ORIGIN      
        1 attaattttg acgtttattt ctattcggcg cgaacaaaaa aatattcata aaagcggttc
       61 ggcttaataa gtagtaatca attagatttt ggaggatagg aaatgaccaa gaaattaatt
      121 tcggttctgc tagcaggttg tttttgctta tttgtatttg ccggcgcgac cttcgctcag
      181 ggcacgactt cgcgcgtgac cggaaccgtc acggacagct ccggcgcagc cgtttccggc
      241 gcgaccgtga ccgttacgaa tgaagcgacg ggagtagcct taaccacgga gacgagcgaa
      301 aacggaactt atattttcga cttgatccag gtcggaagct acacgttaac ggttgaaaag
      361 gctggtttta aaaaactggt ttcaaccaaa aacacggtca acatcaatca accggcgacg
      421 atcaacgcgg cattggaaat aggcgatgtt tcggcgaccg tcaatgtcga aagcaccgtc
      481 gagcaggtgc agaccagcac ttccggcaat atcggcagca cgatcgagca aaggactctg
      541 gaaagtttgc cgattgtcgg aacgcgcggg cgtaatcctc tcgatttatt aaactttcag
      601 ccgggcgtcg ttttcggcgg aaacacgggc ggcgcagtca acgttcacgg ctcgcgcgac
      661 cgcgcgttta attttacgct ggacggaatc gatataaacg attcgagcgc gggcggctca
      721 aactttactc cgctgcgacc aaatccggat tcggttcagg aatttcaaat tatcaccagt
      781 aactttacgg cggaactcgg gcgcagcagc ggcgcgcagg tgacgctggt cacgcgctcg
      841 ggcacgaatc gtttcagcgg aaatttgttt gaatattacc aaacgcccga tttcaatgcg
      901 aaaagttacc cgataaccat cgccggactt gccaaagaac agttcgttca gcacattttc
      961 ggcggcagcc tcggcggtcc gctgtttaat ccgggcttcg gcgaaggcac gaaagccgga
     1021 tggctcagag acaaagcgtt tttcttcgtc aacctgcaaa tgctgcgcgc atacgacacg
     1081 gcgctggtga cccgcaccgt ttatacgcag caggcgcggc agggaatatt cagatacgtt
     1141 cagggcaggg aaacgataaa cggtcaggtc gtcacttacc agaatgctcc ggcgggcgct
     1201 tccaatgctt cggtcgacgc aaacggaaat ccgatcagac cggtttgcgg cggctctatt
     1261 acggtcaatt gtataaacac ttacaatatc gcgaccaccg cgccgatcag catcgacccg
     1321 acgctcgccg gatttatcaa cgcgatgccg ctgccgaaca attttacgac gggcgacggc
     1381 ttgaacaccg ccggatttaa tttcgcctcg ccgcagcgcg agcggcaata cgattttgtc
     1441 accaaacttg attttaaggt ttctgacacg aatatgttct acatcaggta tgcgcagggc
     1501 gagcaaagct cgttcggcga ttcgggaaac ggcgggcgtc cgatttttcc cgattctccg
     1561 aatctggtcg ataccatccg cactccgaaa aatctggcgg tcaactggcg attttcgccg
     1621 acggcgcgct ttaccaacga atttgttttc ggaatcagta aattcgcctt tagcttcgat
     1681 acgcccgaac ccgacccgag catacctttt acctttttga ccatcgctac gccgaacacg
     1741 aatttttcgt ataacgcgcg gcgtctgcgc accttgcagt ttgtcgataa cctgacgttc
     1801 gatttttcgc cgcacgtcat taaagcgggt gtgaactttc gtttcggtcg ccacatcgac
     1861 gatcgttcga cggtttcggg aacgaacgtc gagccggtcg tcacattcag cggcgcggcg
     1921 ggctataacg gatttaattt gccgaccgtc agctccgcca ccaatccgac cggaattaat
     1981 accaacgacc tgacgctgct gcaaaatacg gtcaacaatc tgctcggaag aatcggctcg
     2041 atttcgcagg cttttgtttc cgacccgagc aatccgggcg catttgctcc ggctggaacg
     2101 cgctggatta acaaagcctt ttacccggaa ctggattttt acgtgcagga caactggcga
     2161 ttccggcaaa atctggtttt cgatatcgga ttgcgctggg aagccaaatt gaatccgagc
     2221 gtggacggac gcccgatcct ggtgccgaat cagccggtga aattaggcgc ggcgccgacc
     2281 aactcgctgc gctgggtgga aggcgatttg ttcaaaaacg atttcggtaa atttctgcct
     2341 tcgctcggct ttgcgtggga cccttttaaa accggcaaaa cttctattcg cgccaactat
     2401 cgcatttcgt cggacagaat agccagcttt ttattcggtt cttccatttt tcaaagcacg
     2461 ccgggaaaca acgtcggcgc gacgaactcc gctttcggtc agggcggcgg gctttacaga
     2521 aatctcggaa cggtgctgtc gacgctcgca ccgacttcgt cgccggacat tttacggcag
     2581 ccgcccgctt tcggaaccgg ctcgattaac gtcatcgacc cggatttgaa atatccgatg
     2641 gttcattcct ggtcgctcag ttttcaaaga gagctctgga aaaacaatgt tctcgaagta
     2701 aattatatcg ggaaaaaagg cacgcatctg ctcggcggat acaacgccaa tcaggtgaac
     2761 atcttcgcgg cggcggcggg cgagaatttc ctcgaagctt ttaataaaat cagggaagat
     2821 attcgtatca acggcacgac caatattact tataacagcc cgctgattaa cgctttgatg
     2881 acgggaagcc cgacggacaa cgacggcacg gcgagatttc gcgcgctggt gacgactaat
     2941 acgattacgc agggcaacgt cgcttcggcg gcgctgaccg tctcgcagcg aacctgtcag
     3001 gcgcctgacg tgacggcggg agtttgcacc aacgcgcagc tcggcagaag attggtcgag
     3061 ctgggcggat tcacttatct gcttcagcct tttccgcagt ttaccggcgg tctgaacgtt
     3121 ttcgacagca gcgattactc gttctacaac gctctggaaa ttatcttcaa gcgtcgtatc
     3181 gctggcggct tgggttttca gctggcttat actctgtcca aatcgatgga taatcgctcg
     3241 tgggaccctt cgctcagcac ggttacgacc ggctcggttc aatccgcgtc gagcacgccg
     3301 tttgatttgc gcgaccgaag gctgaattac agctggtcgg attttgaccg ccgccacgtt
     3361 tttcagggaa cgtatgtcta tgaactgccg ttcggcaaag acagatggct ttcgagcggc
     3421 aataaagctg tcgactacgt tttcggcggc tggcagttgt cgggaacgat tatctggatg
     3481 tccggcagac cgtttacggt ttattccggc gtcaatactg tcagcaacgt cgtgcagtcg
     3541 acggctgatt gcagcggctg caccagaaac agcgggcagc tgatattgga aaacggcaga
     3601 aatttctggt ttgacgccgc gacgcgcgcg atgttttcgg cgcccgcgcc cggctcggtc
     3661 ggaaatacgg gacgcaattt ctttctcgcg ccgcgttatt tccagacgga cgcttcgctg
     3721 tcgaaaaaat tccgctttac cgagcggatg agtttcgatg tgcgcgtcga tgcgcgaaat
     3781 ctgaccaata atccttcgtt cgataatccg acggcgctca ttacttcgaa cattttcggg
     3841 cgaattaacg acagcgtgac gaataacgcg cgaagaattc agctatccgg caaatttaat
     3901 ttctaaagtg aaattaaacg agcaaaataa aaactctgtg accgttaaaa gtcacagagt
     3961 ttttttcgtc cgcgcggcaa ccttaaataa attcatcgca tcaatattaa cgggctaaga
     4021 gtcggaatca taagaactga aaatgtgagc gcggctttaa gtctttctaa ttcctcctgt
     4081 tatagctgcc tgccggagca tgtgacccgg caggcttttt ttgttcgcaa acgtaccggg
     4141 gattttgccg ccgcaaaaga tttatatttt atttttttct ttgccgccgc aaaattttct
     4201 tgccttttta taatcgctaa attacaattc aaaaaaaatt acactgcccg cgccaagccg
     4261 tattgccgtc ttatattcag tttccgccga tgtatatttt gaaggataaa ggaaaaataa
     4321 aatgatttta aaaaaagagc tgacccaaag tcggtcaaat tttgtatttg ataatattaa
     4381 tcaaacagct cgccgggcat tcgggctttt cgttgttttg ctcgccggga tgataatttt
     4441 cagttccgta gtcgaaacgg cggaagctgc gactttcacg gtcacgaaaa cggggatgac
     4501 gaacgacggc gtctgcaatg ccga
//
LOCUS       DASWNE010000144         2569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_391524, whole genome shotgun sequence.
ACCESSION   DASWNE010000144 DASWNE010000000
VERSION     DASWNE010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391524"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="VGB68_02970"
     CDS             complement(<1..978)
                     /locus_tag="VGB68_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0048221.1"
                     /translation="MLELKNITKTFGDVVANNDVSINIEKGTIHAIVGENGAGKSTIM
                     RIAYGFYTADSGEILVDGKTVNIRNPHDAIALGIGMVHQHFMLVDTMTVAENIILGAE
                     TGGAANLDLDKANRDILALSNELRLGVNPKASIEDLSVGAQQRVELLKALYRNAQLLI
                     LDEPTAVLTPQETEDFFNILRRMREAGKTIVIITHKLEEVLAISENVTVMRDGRVVGN
                     VKTAETSAKDLARMIVGRDVLLRVEKTYAAPRDNILNVRNLSVVGKHGEALDNISFGV
                     RGGEIVGIAGIEGNGQTELIEAVAGLIPISAGSIELFGKNITNLSARRRK"
     gene            complement(984..2036)
                     /locus_tag="VGB68_02975"
     CDS             complement(984..2036)
                     /locus_tag="VGB68_02975"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY0048222.1"
                     /translation="MKIKILSVLLIFVLSVLLSSCGQTEAESNGKIKVGIVFDIGGKN
                     DRSFNAAAWEGVKRAEKDLDIVLRDVEPGNPTSIEPAMRAFAEENFDLIIGVGFAQGP
                     IMQKVANDYPNIKFAIVDGVIFEADGKTPKQNVASLVFREHEGSYLVGMIAAAKSKTG
                     KLGFVGGMDIPLIHKFETGYEEGARSVNPNIQVFDNYVGVTDSAWNNPGKGKELALNQ
                     IEKGADIIFTAAGNSGLGAFDAVEQYGKDANGQANKFVIGVDSNQNGVKPGFVLTSMV
                     KRVDNAVYDVVKEVKNGNFRSGFHAFGLDKDGVAYAMDSHNAGLISEDILKRVEEAKG
                     RIVAGEIKVTDAMAAK"
     gene            complement(2201..>2569)
                     /locus_tag="VGB68_02980"
     CDS             complement(2201..>2569)
                     /locus_tag="VGB68_02980"
                     /inference="COORDINATES: protein motif:HMM:NF019448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048223.1"
                     /translation="KLQGGNPKICDEPGILLEQSIIEVPIKCPSGGFIDEIDTTEIGK
                     AISSIGGGRLKVEDKIDAAVGYACRKKLGEKVAEHEVLGTLFCRSEVQAAKVLAKLQM
                     AYKIGDEKPVRKFELIKEVI"
BASE COUNT          543 a          719 c          618 g          689 t
ORIGIN      
        1 cttgcgtcgc cgcgccgaaa gattcgtaat atttttgccg aaaagctcga ttgagcccgc
       61 cgaaatcgga atcaaaccgg cgaccgcctc gatcagctcg gtctgcccgt tgccttcgat
      121 tccggcgatg ccgacgattt cgccgccgcg aacgccgaac gaaatgttat cgagcgcttc
      181 gccgtgtttg ccgacgaccg ataaattccg cacatttaaa atattgtcgc gcggagcggc
      241 atacgttttt tcgacgcgca gtaaaacgtc gcgcccgacg atcatccgcg ccaaatcctt
      301 cgcgctggtt tccgccgttt tcacattgcc gacgactcgc ccgtcgcgca tcacggtcac
      361 gttttcggaa atcgccagaa cttcttccag cttgtgcgtg atgatgacga tggttttgcc
      421 ggcttcgcgc atccggcgca ggatattgaa aaaatcttcc gtttcctgcg gcgtcaaaac
      481 ggcggtcggc tcgtccagaa tcaagagctg cgcgttgcgg taaagcgctt tcagaagctc
      541 gacccgctgc tgcgcgccga ccgacaaatc ttcgatgctc gccttcggat tgacgcccaa
      601 gcgtaattca ttggacaaag ccagaatgtc ccggttcgcc ttgtccaaat ccagattcgc
      661 cgcgccgccc gtctccgcgc ctaagattat attttcggcg acggtcatcg tatcgacgag
      721 cataaaatgc tggtgaacca tcccgattcc gagcgcaatg gcgtcgtgcg gatttctgat
      781 atttacggtt ttcccgtcaa ccagtatctc gcccgaatcc gccgtgtaaa aaccgtaggc
      841 gatgcgcata atcgtcgatt tgcccgcgcc gttttcgccg acaatggcgt gaatcgtgcc
      901 tttttcgata ttgatggaaa cgtcgttgtt ggcgacgaca tcgccgaaag ttttagtgat
      961 atttttaagt tccagcattt taattatttc gccgccatcg cgtccgtcac cttgatttcg
     1021 cccgccacga ttctgccttt ggcttcttcg acccgcttta aaatatcttc ggaaatcaag
     1081 ccggcgttgt gactgtccat cgcgtacgcc acgccgtcct tgtccagtcc gaaagcgtga
     1141 aagccgcttc ggaaatttcc gtttttgact tccttgacca cgtcgtagac cgcattatcg
     1201 acgcgtttga ccatcgaggt taaaacgaat ccgggtttga cgccgttctg gtttgaatcg
     1261 acgccgatga cgaatttgtt tgcctgaccg tttgcgtctt tgccgtattg ctcgaccgcg
     1321 tcgaacgcgc cgagaccgga gtttccagcc gccgtgaaga ttatgtccgc gcctttttcg
     1381 atttgattca gagccagttc cttgcctttg cccggattgt tccacgcgct gtcggtgacg
     1441 ccgacgtaat tgtcaaagac ctgaatattc gggttgacgc tccgcgcgcc ttcttcgtag
     1501 ccggtttcaa atttatgaat caggggaata tccattccgc cgacaaagcc gagctttccg
     1561 gttttcgatt ttgccgccgc aatcatcccg acgagatatg agccttcgtg ctcgcggaaa
     1621 accagggatg cgacattctg tttcggcgtt ttgccgtcgg cttcgaaaat cacgccgtcg
     1681 acgatggcga atttgatgtt cggataatcg ttggcgacct tttgcataat cggaccctgc
     1741 gcgaagccga cgccgattat caaatcaaaa ttttcttcgg caaaggcgcg catcgccggc
     1801 tcaatcgaag tcgggttgcc cggctccacg tcgcgcagaa cgatgtccaa atccttctcg
     1861 gcgcgcttga cgccttccca agccgcggcg ttaaacgaac ggtcgttttt gccgccgatg
     1921 tcgaaaacga ttccgacctt gattttgccg ttcgattcgg cttccgtttg cccgcacgaa
     1981 gaaaggagca cggacaaaac aaaaatgagc agaaccgata aaattttgat tttcatatct
     2041 ttttaataac cggaaattca aatcacagcg gaaattcaaa caggatagac aggatgaaca
     2101 ggataaaatg cgcgtcgatt cggtttttaa aattcaatta agctgtgagc tattaaaata
     2161 atcctgttca tcctgtatat cctgtttgaa tattccctaa tcaaataacc tctttgatta
     2221 attcaaattt tctgaccggc ttttcgtctc caattttata cgccatttgc aatttggcaa
     2281 gcacttttgc cgcctgcact tcgctgcggc agaaaagcgt gcctaaaact tcgtgttcgg
     2341 cgaccttttc gcccagtttt tttcggcagg cgtagccgac cgccgcgtcg attttgtctt
     2401 cgaccttcaa tcgcccgccg ccgattgacg aaatcgcttt gccgatttcc gtcgtgtcga
     2461 tttcgtcgat aaacccgccg gacgggcatt ttatcggaac ttcgatgatg ctttgctcca
     2521 aaaggattcc gggttcgtcg cagattttcg ggttgccgcc ttgcagttt
//
LOCUS       DASWNE010000145         1955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_4858, whole genome shotgun sequence.
ACCESSION   DASWNE010000145 DASWNE010000000
VERSION     DASWNE010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4858"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..1177
                     /gene="miaA"
                     /locus_tag="VGB68_02985"
     CDS             254..1177
                     /gene="miaA"
                     /locus_tag="VGB68_02985"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEY0048224.1"
                     /translation="MKNPIYSIVGPTASGKTEIGVELALRVGGEIINCDSVQIYKEIQ
                     IATAKPSEEEKRGVPHHLIDYVAPEINYTAADWAADATAKIYEIEARAKIPILVGGTG
                     FYLRTLRQPFFESPKTDERLREKLRKIKEKKGAEHLHKILRKIDKASAEKLFPRDYVR
                     VMRALEVYFQTGNRLSEIQPNRAAPPEFAGRIKIFALSPPRDLLYERINERAEKHFEK
                     GLVAEVRNLREKGLRDDTNALGAHGYRRVCEFLRGERTLESAIEQTKQDVRNYAKRQL
                     TWFRREAGVVWLEGFGDQRETRENLWKKLEI"
     gene            1266..1544
                     /gene="hfq"
                     /locus_tag="VGB68_02990"
     CDS             1266..1544
                     /gene="hfq"
                     /locus_tag="VGB68_02990"
                     /inference="COORDINATES: protein motif:HMM:NF028518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEY0048225.1"
                     /translation="MEKAPSQNIQDAFLNTARRERMTVLIHLLHGATLSGRIKSFDKF
                     SVLLDVGGQDFLIFKHAISTISQEQRKRTDFAPAAASTTTTTSATTAE"
     gene            1550..>1955
                     /gene="tmk"
                     /locus_tag="VGB68_02995"
     CDS             1550..>1955
                     /gene="tmk"
                     /locus_tag="VGB68_02995"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEY0048226.1"
                     /translation="MRGKFITFEGIDGSGKSTQARLLAGELRLRGYDVLATMEPGGTP
                     LGRRLREAFLETEEEVAPLAELLLFAADRAQHVEFLVKPALQDGKIVVSDRFADATAA
                     YQGAGRGFPEKTINQIVNLATRGLQPDLTLFFD"
BASE COUNT          527 a          496 c          501 g          431 t
ORIGIN      
        1 ggaaaagata taaccgaaat gccgaaggca gaacaaaatt gagattgcgc cctatattaa
       61 tgcatcaaaa gcctttgatg tcaataactt tacgggtttc aagacgcttc tccggcttga
      121 taggctgttg ccctttgctt ctgaattttc aattcaaact tttgattctc ataaattaac
      181 gcgccgcctt gggtctgaaa accatcaatg aaagtgtaaa atttttacga gagagttagt
      241 ccgattagtt tttatgaaaa acccgattta ctcaatcgtc ggaccgaccg cttcgggcaa
      301 aaccgaaatc ggcgtcgagc ttgcgctgcg cgtcggcggc gagataatta attgcgattc
      361 cgtccagatt tataaggaaa tccaaatcgc cacggcgaaa ccgagcgaag aagaaaaacg
      421 cggcgtcccg catcatttga ttgattacgt cgcgccggaa atcaattaca cggcggcgga
      481 ctgggcggcg gacgcgaccg cgaaaatcta cgaaatcgaa gcgcgcgcca aaattccgat
      541 tctcgtcggc ggaaccggtt tttatctgag gactctgcgc cagccttttt ttgaaagccc
      601 gaaaaccgac gagcgattac gggaaaaatt aagaaaaatc aaagagaaaa aaggcgcgga
      661 gcatttgcat aaaattcttc gaaaaatcga taaagcgtct gccgaaaaac ttttcccgcg
      721 cgattacgtc cgcgttatgc gcgctctgga agtttatttt caaaccggaa atcgtctttc
      781 cgaaatccag ccgaatcgcg ccgcgccgcc cgaatttgcc ggaagaataa agattttcgc
      841 tttaagcccg ccgcgcgatt tgctttacga gagaatcaac gagcgcgccg aaaaacattt
      901 tgaaaaaggc ttggtcgcag aagtccgcaa tttgcgcgaa aaaggtttgc gcgacgatac
      961 caacgcgctc ggcgcgcacg gctacaggcg cgtctgcgaa tttttgcgcg gcgagcgaac
     1021 gctggaaagc gcgattgagc agaccaaaca ggacgtgcga aattacgcca agcggcaatt
     1081 gacctggttt cggcgcgagg ctggcgtcgt ctggctcgaa ggcttcggcg accagcgcga
     1141 aacgcgggaa aatttatgga aaaagctgga aatctaaggc ttgtgaaatt tttcttgtca
     1201 gcccgcctaa atttgctata atcaatttgc agtcataaat ccaagtcaat aaaagagaaa
     1261 tagtcatgga aaaagctccg tcgcagaaca tccaagatgc ttttttgaac acggctcgcc
     1321 gcgaaagaat gacagtgctg attcatcttc tgcacggcgc aacgctttcc ggcaggatta
     1381 aaagcttcga taaattttcg gttttactgg acgtcggcgg gcaggatttt ctcattttca
     1441 aacacgccat ttcgaccatt tcacaggaac aacgaaaaag aaccgatttc gcgcccgcag
     1501 cggcatcaac cacgaccacc acatcggcaa cgacggcaga gtaaaaatct tgcgcgggaa
     1561 atttatcact ttcgaaggca ttgacggcag cggcaaatca acgcaagcgc gcctgcttgc
     1621 cggtgaattg cgcctgcgag gctacgacgt tctggcgacg atggagcccg gcggaacgcc
     1681 gctcggacgc cgcctgcgcg aagcctttct ggaaaccgaa gaagaggtcg cgccgctcgc
     1741 cgagcttctg cttttcgccg ccgaccgcgc gcagcacgtc gagtttctgg ttaaacccgc
     1801 tctgcaggac ggaaaaatcg tcgtttccga ccgttttgcc gacgcgaccg cggcgtatca
     1861 gggcgcgggg cgcggctttc ccgaaaaaac gataaatcag attgtgaatt tggcgacccg
     1921 cgggctccaa ccggatttga ctctgttttt tgatt
//
LOCUS       DASWNE010000146         3666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_675189, whole genome shotgun sequence.
ACCESSION   DASWNE010000146 DASWNE010000000
VERSION     DASWNE010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675189"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="VGB68_03000"
     CDS             <1..990
                     /locus_tag="VGB68_03000"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY0048227.1"
                     /translation="IKETITNLGLEYRLLTQFTSFVAVEERIVTDGGQPRRIEVPVEL
                     PDGVSREGVFGDLKSDRLETRAVRLNKLQYLRPTPRKANDARNIAPTLPARKVKREAS
                     KAYEDWSDKNVAYIIGGKEKQAVGEKNASEIIKAASLPKPEYPSAAKAVKATGAVNVQ
                     TTIDENGNVVFAKASGGHPLLQQAAETSAKNAKFIVTKGALEIVKITGVLVYDFTDEL
                     NITVSPELQNVQVEVKPNKLHSAVRALVERLKNNQSPPAAGEAKFVKDGKAEITIRLK
                     ELKPETIAALKALGFEALAEMPSAKAVVGRVAPEKLAALAEIEAVTFISPQNR"
     gene            1251..2798
                     /locus_tag="VGB68_03005"
     CDS             1251..2798
                     /locus_tag="VGB68_03005"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEY0048228.1"
                     /translation="MLKRVERREKSWDIIIIGGGASGVGCAVDAASRGYDVLLLEQSD
                     FGKGTSSRSTKLIHGGVRYLEQGNISLVMEALKERGILRQNAPHLVQDLAFVVPNYDW
                     WEMPFYGVGLKIYDLLAAKYRFGKSKILSKKEILKRLPTIKTEGLRGGVIYYDGQFDD
                     ARLLINLAATAAQQNATVLNYARVFGLTKDATGFIDGLNFVDEESGQVFQAKAKVVIN
                     AAGAFCDAIRRMSDDNAKPLISPSQGIHLVFRREFLPTENAIMIPHTTDGRVLFAIPW
                     HGHTLVGTTDTPVAKAELEPSALEEEIEFVLETAGKYLHKPPQRKDVLSVFAGIRPLV
                     KNGGGKNTATLSRDHTIQIDDAGLITLTGGKWTTYRHMAEDCINQAAVLAKLPEKDCA
                     TKDLKIHGFIENAERFGDLAIYGADAAKIQNLIETDKSLSEKLHADLPYTVAEVVWSI
                     RKESARTIEDVLARRTRALFLNARAAIEIAPKIGKIMARELSKDESWIEKQIDEFKET
                     AEKYLIR"
     gene            complement(2859..3113)
                     /locus_tag="VGB68_03010"
     CDS             complement(2859..3113)
                     /locus_tag="VGB68_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048229.1"
                     /translation="MLPILAMLKQRDFPAGLPAGSFFAGAAVLMAIQTAKSYRNGFMY
                     CGVTEKVYRNQNPLKFKIWLAIQSTFVLLLAAFSLCSFLG"
     gene            complement(3152..>3666)
                     /gene="rph"
                     /locus_tag="VGB68_03015"
     CDS             complement(3152..>3666)
                     /gene="rph"
                     /locus_tag="VGB68_03015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEY0048230.1"
                     /translation="PRATQSRTQRETQRPSGRTQEIQRLIGRSLRAVVDMKKLGERQI
                     FVDCDVILADGGTRCASITGAYVALALACKKIVSQGIVKTSPLLSEVAAVSVGIIGGT
                     PILDLCYAEDSNAEVDMNIVCTGAGKFIEIQGTAEREPFTGEQMTEMLALAEIGINQL
                     FTIQRAILNV"
BASE COUNT          986 a          897 c          976 g          807 t
ORIGIN      
        1 attaaagaaa cgattacgaa tctggggctt gaatatcgtc tgctgacgca gtttacgtcg
       61 tttgtcgcgg tcgaggaaag aatcgtcacc gacggcgggc agccgcgccg catcgaagtt
      121 ccggtcgaat tgccggacgg cgtcagccgt gaaggtgttt tcggcgattt aaagtctgac
      181 aggctggaga ctcgggcggt tcgtctgaat aaattacaat atttacgacc tactccaaga
      241 aaggcgaatg acgcgagaaa catcgcgccc acgcttcccg ccagaaaggt aaagagagaa
      301 gcttccaaag cttacgaaga ctggtcggat aaaaatgtcg catacataat cggcggcaag
      361 gaaaaacagg cggtcggcga aaaaaacgct tcggaaatta tcaaagcggc gagcctgccg
      421 aaacccgaat acccgagcgc cgccaaagcc gtaaaagcga cgggcgcggt gaatgttcag
      481 acaacgattg acgaaaacgg caatgtcgtt ttcgcgaaag cctccggcgg gcatccattg
      541 cttcagcaag cggcggaaac ctcggcgaaa aacgctaaat ttatcgttac aaaaggcgcg
      601 cttgaaatag taaaaattac gggcgttctg gtctacgatt ttacggatga gctgaatata
      661 accgtttcgc cggagctgca aaatgttcag gtcgaagtca aaccgaacaa gcttcattcg
      721 gcggtcaggg cgctggtcga gcgcttgaaa aacaatcaat cgccgccggc ggcgggcgaa
      781 gcgaaattcg tcaaagacgg gaaagccgag atcaccattc gtttgaaaga gctaaagccg
      841 gaaacaatcg cggcgctcaa agcgctcggt tttgaagccc tggcggagat gccttcggca
      901 aaagccgtcg tcgggcgagt agcgccggaa aaactggcgg ctctggcgga gattgaagcc
      961 gtcacgttta tttcgccgca gaatcgctag cttttaaaag cgtttcacca cagagacacg
     1021 gagacacaga gatttttagg aaagaattaa cagggatgaa ggggattaag cagataaaga
     1081 caaaatcatt aatccctttc ttttttatcc ctgtctatcc tcttcatccc tgttaattct
     1141 ttctctatgt ctccgcgtct gtctggtgaa aaattttatg agcggtcaat gttgcgaaac
     1201 ttgaccgctt ttttcttttc ctttcaaatg tttatatgaa tcgtgcggaa atgctcaaac
     1261 gcgtcgaaag gcgcgaaaaa agttgggaca ttattattat cggcggcggc gcgagcggcg
     1321 tcggctgcgc ggttgacgcc gcttcgcgcg gttacgacgt tttgcttctg gagcagagcg
     1381 atttcggcaa gggaacttcg tcgcgctcga caaaactgat tcacggcggc gtccgttatc
     1441 tcgaacaggg aaatatctcg ctcgttatgg aagcgctgaa agaacgcgga atcttgcggc
     1501 agaacgcgcc gcatctggtg caggatttgg ctttcgtcgt gcctaattac gattggtggg
     1561 aaatgccttt ttacggcgtc gggctgaaaa tttacgattt attagccgcc aaataccgtt
     1621 tcggaaaatc gaagattctg tcgaaaaaag aaattctgaa acgcctgccg acgataaaaa
     1681 ccgaaggctt gcgcggcggc gtgatttatt acgacggtca atttgacgac gcgcgccttt
     1741 tgataaatct ggcggcaacg gcggcgcaac aaaacgcgac cgttttaaac tacgcgcgcg
     1801 ttttcggttt gacgaaagac gcgacgggct ttatcgacgg cttgaatttt gtcgatgaag
     1861 aatcgggaca ggtttttcag gcgaaagcaa aagtcgttat caacgcggca ggcgcgtttt
     1921 gcgacgcgat tcgccggatg tcggacgata acgcaaagcc tctaatctcg ccgagccagg
     1981 gaattcatct cgttttccgc cgcgaatttt tgccgaccga aaatgcgatt atgattccgc
     2041 acacgaccga cggcagagtt ctgttcgcca tcccgtggca cggtcatacg ctggtcggca
     2101 cgaccgacac gccggttgca aaagccgaat tggagccgtc cgctctggaa gaagaaatcg
     2161 aattcgttct ggaaaccgcc ggaaaatatt tacataaacc gccgcaaaga aaagacgttt
     2221 tgagcgtttt tgccggcatt cgcccgctgg taaaaaacgg cggcggcaaa aatacggcga
     2281 ctctttcgcg cgaccacacg attcagattg acgacgccgg cttgataacc ttgacgggcg
     2341 gaaaatggac gacctaccgg catatggcgg aagattgcat caatcaagcc gccgttttag
     2401 caaagctgcc ggaaaaagac tgcgcgacga aagatttaaa aattcacggg tttatcgaga
     2461 acgcggaacg attcggcgat ttggcgattt acggcgcgga cgccgcaaaa attcaaaatt
     2521 tgattgaaac cgataaaagc ctgagtgaaa aattacacgc agatttgcct tatacggttg
     2581 cggaagtcgt ctggtcaatc agaaaagaat cggcgcggac aatcgaagac gttctggctc
     2641 gacgaacgcg agcgctgttt ttaaacgccc gcgcggcaat cgaaatcgcg ccgaaaatcg
     2701 gcaaaattat ggcgcgtgaa ttaagcaaag atgaaagctg gattgaaaag caaatcgacg
     2761 aatttaagga aaccgccgaa aaatatttaa tacgatgact gaaaaaaatg atgaaatttc
     2821 gtcattggaa atggattttc aaggctgtat ctagaacttt aaccgagaaa cgaacaaaga
     2881 gaaaacgctg caagaagaag aacaaacgtc gattgtattg ccagccaaat cttaaacttt
     2941 aatggattct gatttcgata aaccttctca gtcaccccgc agtacataaa tccatttcga
     3001 taggatttgg cggtttgaat agccattaac acggctgcgc ccgcaaagaa agagccagcc
     3061 ggcagtccgg cgggaaagtc tcgctgtttg agcatcgcaa gtatcggcag cataactttc
     3121 cgttatattt aacgccaaaa cagaattctg tctagacgtt cagaatggcg cgctgaatcg
     3181 taaaaagctg gttgatgccg atttccgcca gcgccagcat ctcggtcatc tgctcgccgg
     3241 taaacggctc gcgctccgcc gtgccctgaa tctcgataaa ttttcccgcg cccgtgcaga
     3301 cgatgttcat atcgacttcg gcgttcgaat cttccgcgta gcacaaatcc aaaatcggcg
     3361 tgcctccgat gatgccgacg ctgaccgccg cgacctcgct caagagcggc gaggttttga
     3421 cgatgccctg cgaaacgatt tttttgcagg cgagcgccag cgcgacataa gcgccggtaa
     3481 tcgaagcgca gcgcgttccg ccgtcggcta aaatcacgtc gcagtcgaca aaaatctgcc
     3541 gctcgccgag ctttttcata tcgacgacgg cgcgcaggct tctgccgatc agccgttgaa
     3601 tttcctgcgt tctgcctgac ggtcgctgag tctcgcgctg cgtgcggctt tgcgtcgcgc
     3661 gcggca
//
LOCUS       DASWNE010000147         2466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_110660, whole genome shotgun sequence.
ACCESSION   DASWNE010000147 DASWNE010000000
VERSION     DASWNE010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110660"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VGB68_03020"
     CDS             <1..546
                     /locus_tag="VGB68_03020"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEY0048231.1"
                     /translation="AENFSPSPEFVLMFLPSEAIYSAALEHDADLIEYGALKNVIIAT
                     PSTLIALLKAAAYGWQQEAVTKEVKQVGELGKQLYKRLVSMTEHIIKVGESLDKSVKS
                     YNSMIGSIERSVLPSARRFKDLQVIGGASEEIAALETIERVSREFQAGEFKSAAAGIN
                     QNVSESNQAGNSDDENDLLNL"
     gene            722..>2466
                     /locus_tag="VGB68_03025"
     CDS             722..>2466
                     /locus_tag="VGB68_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048232.1"
                     /translation="MLRQLRQQRRNFRDASEHIKTSVKNVEAGKGLSLGDYEEINKYF
                     DAEATGGLNGAWREFKSHFLLRKLDSGENLYWSTRNAEEAFSERKLVTEKLSRDYYQS
                     LPGIITGIGLMTTFFAILVALLDVRLVDNRVQGLELLIQGLSGKFITSVVALLCATVF
                     VFFEKNRFHALEKSQLKLASLIDLTVPQLSPVHILVDLQTNFGQLHKDTNLGFAGLRD
                     EIKQGIESIVHADSANIGQLQSEIKQGIDSIRQSTILLQEDVANQTVCIQDFNTSLAP
                     MLQQTFDESMTPAMNRMIKSIEDMNEFLRSVEANKSDSITDSIKELLTNLQTSLTGSI
                     SEMGGKFNESLSGNAQQQFASVADSLLNTTAILDGMNQQFSSSQNALQEMIIQARSTT
                     QEQININHNQLNSLAETIGSLMIDLSERVNNLGEKMSASVQANSENAVGAADRVIKRA
                     NEWSTENAAQLTRLLEVHQSQFERVVQTRELLDGTIVKFNGSLENLMKISLNSRTSVQ
                     QINAAVTSITSAADKTATTHQSLQKISDSTAEQLEELSSAHHRQREMWTAIHNTMEQY
                     KQTFSTVENSAGALL"
BASE COUNT          728 a          535 c          587 g          616 t
ORIGIN      
        1 gccgaaaatt tcagtccttc accggaattt gtcttgatgt tcctgccaag cgaagcgatt
       61 tacagcgccg ctctcgaaca cgacgcggat ttaatcgaat acggcgcgct caaaaacgtc
      121 attatcgcca cgccttcgac gctcatcgca ctgctcaaag cggcggctta cggctggcag
      181 caggaagcag tgacaaagga agtcaagcaa gtcggcgaac tcggaaaaca attatataaa
      241 aggttggttt caatgacgga acacatcatc aaagtcggcg aatcactaga taaatcggtt
      301 aaaagttaca actcaatgat tggatcaatc gagcgcagcg tattgccgag cgcgcgccgc
      361 tttaaggatt tgcaggtcat cggcggagcg tccgaagaga ttgccgcgct tgaaacaatc
      421 gaaagggtta gccgcgaatt tcaagccggt gaatttaaat ctgcggcagc gggaatcaat
      481 caaaatgtta gcgagtctaa tcaggcggga aattcggatg acgaaaatga tttattgaat
      541 ttgtagtatt acccggttct atgaactcgc cgaaatatat ttcctgttct cagtatttct
      601 taaaacggtt atgttggaat atctcaaatt taaatttaat cttttcggaa ttgacgcacc
      661 cgcttttgcc tgggtcgtcg cggcttttct gattttattt acggctattc atttagaaag
      721 gatgttgcga cagctacgtc agcaacggcg aaatttccgc gatgcgtccg aacatataaa
      781 aacgtcagta aaaaacgtcg aagccggaaa aggtttgtct ctaggtgatt atgaagaaat
      841 caataaatat ttcgacgccg aagcgacggg cggattaaac ggcgcgtggc gcgaattcaa
      901 atcgcatttt ctgctgagaa aacttgacag cggcgaaaat ctttactggt caacgagaaa
      961 cgcagaagaa gccttttccg aaagaaagct ggtcaccgaa aaactgagcc gcgattatta
     1021 tcaatcgctt ccgggaatta tcaccggaat cggattgatg acgacgtttt ttgcgattct
     1081 agtcgctttg ctcgatgtgc gtctggttga taatcgcgta cagggtttgg aacttttaat
     1141 tcaaggttta tcgggtaaat tcatcacttc tgtcgtagca ttgctttgtg cgaccgtttt
     1201 cgtgtttttc gagaaaaatc ggtttcacgc gctggaaaag agtcagttaa aacttgcttc
     1261 gttgattgac ctaacggttc cgcagctctc gcccgtccat attctagttg atttacagac
     1321 gaatttcggt cagttgcaca aagatacgaa tctgggcttt gccggtttgc gtgatgaaat
     1381 aaaacaaggc atcgagtcga ttgttcacgc cgattcggcg aatataggac aattgcaaag
     1441 tgaaataaaa cagggcattg attcgatcag gcaaagcacc attcttttgc aggaggatgt
     1501 cgccaatcaa accgtctgca ttcaggattt taatacgagt ttagcaccaa tgcttcagca
     1561 gacgtttgac gaaagtatga ctccggcaat gaatcggatg atcaaatcaa tcgaagatat
     1621 gaacgaattt ttgcgttccg tcgaagcaaa caaaagcgat tcgattacag attcgattaa
     1681 agaactctta actaatctgc aaacttctct gaccggctcg attagcgaaa tgggcggaaa
     1741 gtttaacgaa tctttatcgg gcaacgcgca gcagcaattt gcgtcggtcg ccgattcgct
     1801 tttgaacacg acggcgattc tcgacggaat gaatcaacag ttttcttcat cgcaaaacgc
     1861 tttgcaggaa atgattatcc aagctcgctc aacgacgcaa gagcaaatca atatcaatca
     1921 taatcaacta aacagtcttg ccgagacaat cggcagtttg atgattgatt tatcggaaag
     1981 agtgaataat ttaggtgaaa aaatgagcgc gtccgtgcag gcgaattcag aaaatgcggt
     2041 tggtgccgcc gacagggtaa tcaaacgcgc taatgagtgg tcgactgaaa acgcggcgca
     2101 gctgacacgg ctcctggaag ttcaccaatc gcaatttgaa agagtcgtcc aaacgcgcga
     2161 actgctcgac gggacaattg ttaaatttaa cggttcgctc gaaaatctga tgaaaatctc
     2221 attaaattcc cgaacttccg ttcaacagat aaatgcggcg gttacttcga taacatcggc
     2281 ggcggataaa acggcgacaa ctcaccagag cttgcagaaa atttccgatt caaccgccga
     2341 gcagcttgaa gaactgagca gcgcgcacca ccggcaaagg gaaatgtgga cggcgataca
     2401 taatactatg gaacaatata aacaaacctt cagcacggtt gaaaattccg cgggtgcgct
     2461 gctcga
//
LOCUS       DASWNE010000148         3250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_605355, whole genome shotgun sequence.
ACCESSION   DASWNE010000148 DASWNE010000000
VERSION     DASWNE010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605355"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..2105)
                     /locus_tag="VGB68_03030"
     CDS             complement(99..2105)
                     /locus_tag="VGB68_03030"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY0048233.1"
                     /translation="MRQFKLFVSLAIMLWATVGVISGQNTPPPLTIRMQPFLTGLSLP
                     VHITNARDGSGRLFVVQQRGIIKVVQPGSNATTDFLNVSSVVSSSGNERGLLGLAFHP
                     QYAANRRFFIYYTRQSDGAIEIAEYETSATNPNQANPAAVRIIITIPHPTFSNHNGGT
                     ILFGPDGYLYAGTGDGGSGNDPNNNAQNINALLGKMIRLDINTPVGQVPAYNIPPTNP
                     YAGATPGADEIYAIGMRNPYRFSFDRGGTNQLWVGDVGQDAIEEVDIIINGGNYGWRV
                     YEGLQCTGLDPSRCATGTNPLMQIPPVFQYSSTGTGNPRCSVTGGYVYRGTLNALPVG
                     SYVYADYCSGEILLWENNQQRLLLDTSRNISSFGEDENGELYVVGLGGTVEKIVRAKA
                     NADFDGDFKTDISVFRPSGGNWYINQSLNNTFRAVQFGANGDVPAPEDYDGDNTTDIA
                     VWRPSNGTFYALRSSNSTFTAQQFGANGDTPVAADYDGDARADFAVFRPAGGNWYRIN
                     SANGAFVATQFGANGDIAVPGDYDGDGKYDQAVFRNSNGFWYLLNSSNGTFSAVQFGA
                     NADTTAQGDFDGDGKTDISVFRSNDGTWYRLNSSNGAFVATQFGTLGDRPVVGDYDGD
                     GRDDIAVFRPQGGFWYRINSSNSQFVAAQFGQNGDLHVPAYDQP"
     gene            complement(2166..2591)
                     /locus_tag="VGB68_03035"
     CDS             complement(2166..2591)
                     /locus_tag="VGB68_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048234.1"
                     /translation="MRRICFAVICCLILLFSNAPAETKIGAAENIRCVTPSVKAALRN
                     SGAVFAGEVLSESKNGSERTFEFRVKRYWKGAGKKKIKVSVHESARYQAWFEVGKSYL
                     VFAGADEDGRLYDSRCSGSKLLSEASEDLKALGRAKIPR"
     gene            complement(2817..3077)
                     /locus_tag="VGB68_03040"
     CDS             complement(2817..3077)
                     /locus_tag="VGB68_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048235.1"
                     /translation="MKNIIQFAVVIYGFMSSSACVYYEIPKPKDWSSTIKQVKEPTTE
                     TIRVDDTESDTVLFWYQSYPSAQPVKVEKVSETRWVVVFEKK"
BASE COUNT          787 a          862 c          779 g          822 t
ORIGIN      
        1 aaccgtcgcg cgagcctttt aaaggataaa ggctaaagga taaaggaaat cattttcctt
       61 agtttttcgc ctttatcctt tagcctttat cctttcgatt acggctggtc gtaagccgga
      121 acatgcaaat cgccgttttg cccgaattgc gccgcgacga actggctatt tgagctattg
      181 atgcgatacc agaagccgcc ctgcggacgg aacacggcga tgtcgtctct gccgtcgccg
      241 tcatagtcgc cgaccaccgg acggtcgccg agcgtgccga actgtgttgc gacgaacgcg
      301 ccgttcgagc tgttcaggcg ataccacgtg ccgtcgttcg aacgaaaaac ggaaatgtcg
      361 gttttgccgt cgccgtcaaa atcgccctgt gccgtcgtgt ctgcattcgc cccgaactgc
      421 actgcgctga aagttccgtt cgacgaattc agcagatacc aaaaaccgtt cgagttgcgg
      481 aaaactgcct ggtcgtattt gccgtcgccg tcatagtcgc ccgggacggc aatgtcgccg
      541 ttcgccccga actgcgtggc gacgaatgcg ccgttcgcac tgttgattct ataccagttg
      601 ccgccggcgg gacgaaacac ggcgaaatcg gctcttgcat cgccgtcgta atccgccgcg
      661 acgggcgtat cgccgttcgc gccgaactgc tgcgcggtaa aagtgctgtt gctgctgcgc
      721 agagcgtaaa acgtgccgtt tgaaggtcgc cagacggcga tatcggtcgt gttgtcgccg
      781 tcgtaatctt cgggcgcggg cacgtcgccg tttgcgccga attggacggc acgaaacgta
      841 ttattcaaac tctggttgat ataccagttg ccgccgctgg gacgaaagac ggaaatgtcg
      901 gttttgaaat cgccgtcgaa atcggcattg gctttagcgc gaacaatttt ctcaaccgtt
      961 ccgcccaatc cgaccacgta aagctcgccg ttttcgtctt cgccgaatga agagatattg
     1021 cgggaagtat cgagaagaag cctctgctgg ttattttccc agagcaaaat ctcgcccgaa
     1081 caataatccg cgtagacata tgagccgacc ggcagagcat tcagcgttcc gcgatagacg
     1141 tagccgcccg tgaccgaaca gcgcggattg ccggtgccgg tgctgctgta ttgaaaaacc
     1201 ggcggaatct gcatcaaagg atttgttccg gtcgcgcatc tggacgggtc gagcccggtg
     1261 cattgcagcc cttcgtaaac gcgccagccg tagttgccgc cgttgatgat aatatcgact
     1321 tcctcaattg cgtcctgacc gacatcgccg acccaaagct ggttggtgcc gccgcggtca
     1381 aacgaaaaac gatacggatt acgcattccg atagcataaa tttcatcggc gccgggcgtc
     1441 gctccggcat agggattggt cggcggaata ttatacgccg gaacctgacc gaccggcgtg
     1501 ttgatgtcga ggcgaatcat tttgccgagc agggcgttaa tgttctgcgc gttgttgttc
     1561 gggtcgttgc ccgaaccgcc gtcgcccgtt ccggcgtaga gatagccgtc cggtccgaac
     1621 aaaatcgttc cgccgttgtg attgctgaaa gtcggatgcg gaatcgtgat gattattctg
     1681 accgccgccg gattggcttg attcggattc gtcgccgaag tttcgtattc ggcgatttcg
     1741 atagcgccgt ccgactgccg cgtgtaataa ataaaaaacc gtcggttcgc cgcatactgc
     1801 gggtgaaagg cgagtccgag aagcccgcgc tcattgcccg acgaactgac gaccgacgag
     1861 acatttaaaa aatccgtcgt cgcgttcgaa cccggctgaa cgaccttgat aatccctcgc
     1921 tgctgcacga cgaaaagccg tcctgacccg tctctcgcat tagtaatatg caccggcaga
     1981 cttagacccg tcaaaaacgg ctgcatccga atcgttaaag gcggcggcgt attctgcccc
     2041 gaaatcacgc ccaccgtcgc ccaaagcata attgccagac ttacaaataa cttaaattgt
     2101 cgcatattcc ccctagaaca tttccaatgt aatcttaatt taaaatcgaa aacttttatt
     2161 ttcgattaac gcggaatttt cgcccgcccc agtgccttta aatcttcgga cgcttcggaa
     2221 agcagtttag aacctgagca cctgctgtcg taaagccgcc cgtcttcgtc tgccccggca
     2281 aaaaccagat agcttttccc gacctcaaac caggcttgat aacgcgcgct ctcgtggacg
     2341 ctgactttaa tttttttctt cccggcgcct ttccaatacc ttttaacccg aaattcaaaa
     2401 gttcgctcgc tcccgttttt actctcgctc aggacttcgc ccgcaaaaac agcgccggaa
     2461 tttcgcaaag ccgcctttac gctcggagtc acgcatctga tattttccgc cgcaccgatt
     2521 ttcgtttcgg caggcgcatt actaaaaagc aaaatcaaac aacagattac ggcaaaacaa
     2581 atacgtcgca taatattttc tcttatagga aactcgggaa ggtaaattga ttttaagcca
     2641 tcacaacgga taaaaacaag aagaatttag caaccgccgc tttgcgccgc aaaataatta
     2701 catttaataa attcaataaa taccggctga ataactcaaa tctctcaaac gacggcatta
     2761 aaccaagata attagctgca ctgtaaaata ggttgttttc agtttttaat gttgaatcat
     2821 tttttttcaa aaacaaccac ccagcgagtc tcgcttactt tttctacttt aactggttga
     2881 gctgaagggt aagattgata ccaaaataga accgtatctg attctgtatc atctactctt
     2941 atagtctctg ttgtaggctc tttaacttgt ttgattgtgc tgctccaatc ttttggtttt
     3001 gggatttcgt aataaacaca ggcagatgag ctcataaatc cgtaaattac gactgcgaat
     3061 tgaattatat ttttcatatt ctattgcggc gtgtttcacg atgattaaaa agatttgttt
     3121 caaaaatgac tgttaaagga aacactgcgc cgtccgactt attcgacatg cgtcgcgatt
     3181 cggagctttc ctatatttta aattgcttgt atgactcgat tcgatcgtta agaatataaa
     3241 tcggacgccg
//
LOCUS       DASWNE010000149         2826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_252555, whole genome shotgun sequence.
ACCESSION   DASWNE010000149 DASWNE010000000
VERSION     DASWNE010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252555"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(240..1190)
                     /locus_tag="VGB68_03045"
     CDS             complement(240..1190)
                     /locus_tag="VGB68_03045"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEY0048236.1"
                     /translation="MISIPLITKTEDFERHFSAGFWRDFAGIICRRHNLSFQNPQRAG
                     GTEHIVFFVDNAFVIKIYTPFRGGFGREKAGLEFARNKTSLITPEILFEGEIEGFKYL
                     ILNRLEGVSMTRESWLGLETRKQARIVSQLSAGLKELHSHDARAIDFDWQKFIAHQAA
                     SVVERQKAGGASPEWLERLPDYLEESLPLLPKDCEDVFLHGDVHFGNLCLVEKRGKWS
                     ISGLFDFADSLKGFHEYDFLAVGVLMIQGQGALQREFFRAYGYAENEIDAGLRRRLML
                     LTILYECSNLRKYALRLKPEAVDLTLDELERAIWNFCDNR"
     gene            1313..2221
                     /locus_tag="VGB68_03050"
     CDS             1313..2221
                     /locus_tag="VGB68_03050"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY0048237.1"
                     /translation="MDSVSAASGGKTSFLSEGVRQMFYSALAFSLANVFVKQLSHLPA
                     MEIVFFRCLLGSIFCFYGLRRAGAGWKGSNRKQLALRGLFGTTALYFFFVTVQNIPLA
                     SAMTIQYLSPIFTTIIAVFLLGESVKRMQWLFYAIAFSGVLFIERFDTRISFFFIIIG
                     VISAFCSGVAYNLVRGLREREHPLTVVLHFQLIGVIAGFLFTIFNWRTPRGWDWLYLF
                     LVGLSSQFGQIFLTNALQKEKAASVAIVNYSGLIYGLLFGWFVFGEAQTIESVIGMSL
                     VVCGVILSIFYGRRQREIEKIESTAG"
     gene            2316..>2826
                     /locus_tag="VGB68_03055"
     CDS             2316..>2826
                     /locus_tag="VGB68_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY0048238.1"
                     /translation="MLPRKKILALILAGGAGGRLGLLTEKRAKPVMPFAGFYRLIDFA
                     LSNCLHSGVSDVWVIEQYELHTLNEHLSNGRPWDLDRTYGGLQVLPPFERNGKKSGDK
                     GGFADGNADAIFRHLDLIEEFNPDILIVLSADHVYKFDFQDAIQTHLEKKAALTMVTT
                     NLPKGENASR"
BASE COUNT          664 a          680 c          696 g          786 t
ORIGIN      
        1 tcgacatctg cgatttttgc gactgatatt ttctgagaac gagcgcgtat tcgtcggcga
       61 gcctcaaaac gggtttggtc tgcgaacaaa cgggcgaagc gccgaatgcg tgaaatgcaa
      121 tgaataaggc ggcggcgagc gcggttttca gatacataca aacctccggc gcgattatac
      181 tttaataact ggtaactgat gcccgataac tattttattt ttaagttttc acttttcagt
      241 tatcggttat cgcaaaaatt ccaaatcgcc cgttccaatt cgtcgagcgt caaatcgacc
      301 gcttccggct tcagacgcaa agcatatttt cgtaaattgc tgcattcgta caaaatcgtc
      361 agaagcatca gacggcggcg caatccggcg tcaatctcgt tctcggcgta accgtaggcg
      421 cgaaaaaatt ctctctgcaa tgcgccctgt ccctgaatca tcaaaacgcc gacggcgaga
      481 aaatcgtatt cgtgaaagcc cttcaaagaa tcggcaaaat cgaacaaacc ggaaatactc
      541 cattttccgc gcttttcaac caggcacaaa ttgccgaaat gaacgtcgcc gtgcagaaag
      601 acatcttcgc agtctttcgg caaaagcggc aggctttcct ctaaataatc cggcagccgc
      661 tcgagccatt cggggctcgc cccgcccgct ttttgtcttt cgacgacgct cgcggcttga
      721 tgtgcgataa atttttgcca gtcgaaatct atcgcccgcg catcgtgcga atgcagttct
      781 tttaatccgg cggaaagctg cgaaacgatt cgcgcctgtt ttcgcgtttc caatccgagc
      841 cagctttctc tggtcatcga aacgccttcc agccgattta aaataagata tttaaagcct
      901 tcaatctcgc cctcgaataa aatttcaggc gtaatcaggc tcgttttatt tcgcgcgaat
      961 tcgagtccgg ctttttcgcg cccgaaaccg ccgcgaaaag gcgtgtaaat tttgataacg
     1021 aaggcattat cgacgaaaaa aacgatgtgc tcggtgccgc ccgcgcgttg cgggttttga
     1081 aaagagagat tgtgcctgcg gcagattatc ccggcaaaat cgcgccagaa cccggcgctg
     1141 aaatgccgct cgaaatcttc cgttttggta attaaaggta tgctaatcac aataaatttt
     1201 cgtgaaaaaa tttcttattt cttttgacgg cgcgtttttt cttttacccg accgataaat
     1261 tacccgaccg ataaatgtta aactaaattt gattagaggc ataatttttt taatggattc
     1321 ggtttcggca gcttcgggcg ggaaaacttc atttttgagc gaaggcgtgc ggcagatgtt
     1381 ttattcggcg ctcgcttttt cgctggcaaa cgttttcgtc aaacagctct cgcatctgcc
     1441 ggcgatggaa atcgtctttt ttcgctgtct gctcggttcg attttttgtt tttacggctt
     1501 gcgccgggcg ggcgcgggct ggaaaggctc gaatcgaaaa caactggctt tgcgcgggct
     1561 tttcggcacg acggcgctgt attttttctt tgtcacggtg cagaatattc cgctcgcttc
     1621 ggcgatgacg attcaatatc tgtcgccgat ttttacgacg attatcgccg tttttctgct
     1681 cggcgaaagc gtcaagcgga tgcaatggct tttttacgcg attgcttttt ccggcgtttt
     1741 gtttatcgaa agattcgaca cgcggatttc gtttttcttt atcattatcg gcgtcatttc
     1801 ggcgttttgc tcgggcgtgg cgtataatct cgtgcgcggt ttgcgcgagc gcgagcatcc
     1861 tttgacggtc gttctgcatt ttcagttaat cggcgtcata gccggatttc tttttacgat
     1921 tttcaactgg cgcacgccgc gcggatggga ttggctttat ttatttttag tcggtttgtc
     1981 ttcgcaattc gggcagattt ttctgaccaa cgcgctgcaa aaggaaaaag cggcgagcgt
     2041 ggcaatcgtc aattattccg gcttgattta cggattactg ttcggctggt ttgttttcgg
     2101 cgaagcgcag acgatagaat cggttatcgg aatgagctta gtcgtctgcg gcgtgattct
     2161 gagtattttc tacggcagaa gacagcgcga aatcgagaaa atcgaatcga cggcgggttg
     2221 ataaaggata aaggcaaagg gataaaggat aaagaataag gaataaattc cctgcttacc
     2281 tttcgccctt cgcctttatc ctttatcctt tagaaatgct tccacgtaaa aaaattctgg
     2341 ctttgattct ggcgggcggc gcgggcgggc ggctcggttt gctgacggaa aaacgcgcca
     2401 agcccgtgat gcctttcgcc ggattttacc ggctgattga tttcgctttg agcaactgcc
     2461 tgcacagcgg cgtttcggac gtttgggtta tcgagcaata cgagcttcac acgctcaacg
     2521 agcatctgtc gaacggcaga ccgtgggatt tagaccgcac ttacggcggc ttgcaggttc
     2581 ttcctccatt cgagcgaaac ggaaaaaaat ccggcgataa aggcggattc gctgacggaa
     2641 acgccgacgc gatttttcga catctcgatt tgattgaaga atttaatccc gatattttaa
     2701 tcgttctgag cgccgaccac gtttacaaat tcgattttca ggacgcgatt cagacgcatc
     2761 tcgaaaaaaa agcggcgctc acaatggtta cgacaaacct tccgaaaggc gaaaatgcct
     2821 cgcgtt
//
LOCUS       DASWNE010000150         2930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_5068, whole genome shotgun sequence.
ACCESSION   DASWNE010000150 DASWNE010000000
VERSION     DASWNE010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5068"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..327
                     /locus_tag="VGB68_03060"
     CDS             40..327
                     /locus_tag="VGB68_03060"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEY0048239.1"
                     /translation="MIEPLQIIEESDSELTIAWSDDSQKTYNAPDLRRACPCASCIDE
                     WTGAKRLKDESVAEDLTIKNIAVVGRYALNFRFSDGHETGIYSFQYLKDLK"
     gene            439..711
                     /locus_tag="VGB68_03065"
     CDS             439..711
                     /locus_tag="VGB68_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048240.1"
                     /translation="MHERDIFDEKPETKRANYVCPHCREANDYEVRWLKRVKKKNPPR
                     QMNEHDRARFNKSRDYMVRVDDMLMCKNMRCRRRFEIPSFQTVVFI"
     gene            836..1225
                     /locus_tag="VGB68_03070"
     CDS             836..1225
                     /locus_tag="VGB68_03070"
                     /inference="COORDINATES: protein motif:HMM:NF039411.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6335 family protein"
                     /protein_id="HEY0048241.1"
                     /translation="MPTKPSENPEYYEYAPVASEDVQDLMKDEISSAPRAPQELRDRL
                     RENNASSPADSGGDIDADWEDVEDSGSESVGGHNPTPDQSDVEANAHAIGIDFQDNEP
                     LDLLDKIEKRDRNRFELDENSKEGDMI"
     gene            complement(1316..>2930)
                     /locus_tag="VGB68_03075"
     CDS             complement(1316..>2930)
                     /locus_tag="VGB68_03075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0048242.1"
                     /translation="HELNIRIKELRTIFDIAPVGLAVAADPSCDVISANSALAAILGI
                     APGDNISVNPANAGNVPYKHLKDGRELVPHELPMQRAVNEKRTIHDDETDIQRADGKL
                     VTIYSYAAPVFDEKGEVVSCIAAHIDVTERKKDERERERQLDLEQSLRLQAEEANRLK
                     DEFLATVSHELRTPLNSIIGWITMLRDGNLAGDNRERALEAIERGAKSQSQLIEDLLD
                     VSRIISGKLGLDAQPVNIVPVINAAVETVRPAAEAKGIELHAITSAKIAMISGDADRL
                     QQIIWNLLSNAIKFTPGGGAVEILLPESDSQVEIIVRDNGKGISRDFLPFVFDRFRQA
                     DGSITRHFTGLGLGLAIVRHLVELHGGTVGVESEGEGKGASFTVRLPSMALPEAGERE
                     HAAEDEFHGDFPVLNDLRILIVDDEDNTRELLKLVFDNCAATVEVAASAAEALEKVKK
                     LPPDVIVSDIGMPDEDGYTLMQKIRLWEKETGREPQIPAIALTAYARAEDRRQALDSG
                     YQMHISKPVEPFELTRLIENLFKSQTERE"
BASE COUNT          682 a          806 c          754 g          688 t
ORIGIN      
        1 tttttttcgt tcatcgttcc gcgttcataa ttcatcatta tgatagagcc gctccaaatc
       61 atcgaagaat ccgattcgga attgaccatt gcctggtcgg acgattcgca gaaaacttat
      121 aacgcgccgg acttgcggcg cgcctgccct tgcgcgagct gtatcgacga atggacgggc
      181 gcgaaaaggt taaaagacga aagcgtcgcc gaagatttga cgattaaaaa catcgccgtc
      241 gtcggtcgct acgcgctgaa ttttcgtttc tcggacggtc acgaaaccgg catttacagc
      301 tttcaatact tgaaggactt gaaataaaac gatgcacgcc gaaacggcga atgatgaatt
      361 tcggatgctg aatctgcaat tccgccttca acgtttcgcc ttcatcgttt ctcccgttct
      421 ttgtggtaaa atatctttat gcacgaacgc gacatctttg atgaaaagcc ggaaaccaaa
      481 cgcgccaatt acgtctgccc gcattgccgc gaagcgaatg attacgaagt ccgctggctc
      541 aagcgtgtaa aaaagaaaaa tccgccgcgc cagatgaacg agcacgaccg cgctcgcttt
      601 aacaaatcgc gcgattatat ggtgcgcgtg gacgatatgc tgatgtgcaa aaatatgcgc
      661 tgtcgccgcc gctttgagat tccgagcttt caaaccgtgg tctttattta attgataatc
      721 gataactgat aattgaaagt gaaaataaaa cagctatcag ctatcagtta tcaactatca
      781 attttcctgt gctattcttt tcttacagca atttatcaag gaggaattat ttagaatgcc
      841 aaccaaaccg agcgagaatc ccgaatatta cgaatacgcg cccgtcgcca gcgaagacgt
      901 gcaggatttg atgaaagacg aaatcagcag cgcgccgcgc gcgccgcagg aattgcgcga
      961 ccggctcaga gaaaataacg cgtcgtcgcc ggcggattcg ggcggcgata tcgacgccga
     1021 ctgggaagac gtcgaagatt ccggctcgga atcggtcggc ggtcataatc cgacgcccga
     1081 ccagtccgat gtggaagcca acgcccacgc catcggcatc gactttcagg ataacgagcc
     1141 gctcgacctt ctggacaaaa tcgaaaagcg cgaccgcaac cgcttcgaat tagacgaaaa
     1201 ttcaaaagaa ggcgatatga tttaaccggc aaaagaaaac agccgcgaaa cggcaaaaaa
     1261 gacgcgaaat aaatcacaaa aatatttcgc gtcttttttt tgtgttcttt cgcggctatt
     1321 ctctttccgt ttgcgatttg aaaagatttt ctatcagcct tgtcagctcg aaaggctcga
     1381 ccggctttga tatatgcatt tgataacctg aatcgagcgc ctgacgccgg tcttcggcgc
     1441 gggcgtaagc ggtcaaagcg attgccggaa tctgcggctc tcgtccggtt tctttttccc
     1501 acagcctgat tttttgcatt aatgtgtagc cgtcttcatc cggcattcca atatccgaaa
     1561 caatcacgtc cggcggcaat tttttaacct tttccaaagc ttcagccgcc gacgcggcga
     1621 cctctaccgt ggcggcgcaa ttgtcgaaaa ccagtttaag aagctctcgc gtgttgtctt
     1681 cgtcgtcgac tatcagaatt cgcaggtcgt ttaagacggg aaaatcgccg tgaaattcgt
     1741 cttcggcggc gtgttctctt tcgcccgctt cgggcagagc catcgacggc agccgcaccg
     1801 taaagctcgc gcctttgcct tcgccctcgc tttcgacgcc gaccgtcccg ccgtgcagct
     1861 cgaccagatg acggacaatc gccagcccca gtccgagccc cgtaaaatgg cgcgtaatcg
     1921 agccgtcggc ttgacgaaaa cggtcgaaga caaacggcag aaaatcacgg ctgatgcctt
     1981 ttccgttgtc gcggacgata atctcgacct gcgaatcgct ttccggcaaa aggatttcga
     2041 ccgcgccgcc tccgggcgta aacttgatgg cattcgacaa aagattccag ataatctgct
     2101 gcagacggtc ggcgtcgccc gaaatcatag cgattttcgc gcttgtaatc gcgtgcagct
     2161 cgatgccttt cgcttccgct gccgggcgca cggtttcgac ggcggcgtta atcaccggaa
     2221 cgatattgac cggctgagcg tccagaccga gcttaccgct gatgatgcgc gaaacgtcca
     2281 gcaaatcctc gattaactgc gactgcgatt tcgcgccgcg ctcgatcgct tcgagcgcgc
     2341 gctcgcggtt gtcgcccgcc agattgccgt cgcggagcat cgttatccaa ccgataatcg
     2401 aattgagcgg cgtgcgcagc tcgtgcgata cggtggcgag aaattcgtct ttcagacgat
     2461 tggcttcttc cgcctgcaag cgcagcgatt gctccaaatc cagctgccgc tcgcgctcgc
     2521 gctcgtcctt tttgcgctcg gtcacgtcga tatgcgcggc gatacagctg acgacttcgc
     2581 ctttttcgtc aaagacgggc gcggcgtagc tgtagattgt gacgagcttt ccgtccgctc
     2641 tttgaatatc cgtttcgtcg tcgtgaatcg ttcttttttc gttgacggcg cgctgcatcg
     2701 gaagctcgtg cggcaccagc tcgcgcccgt ccttcagatg tttatacggc acgtttccgg
     2761 cattggcggg attgacggaa atattatcgc ccggagcgat gccgagaatc gcggcgagcg
     2821 cggaattagc gctgatgaca tcgcagctcg ggtcggcggc gacggcgagt ccgaccggcg
     2881 caatgtcaaa aatcgtccgc aattccttga tgcggatatt cagctcgtga
//
LOCUS       DASWNE010000151         3130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_216129, whole genome shotgun sequence.
ACCESSION   DASWNE010000151 DASWNE010000000
VERSION     DASWNE010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216129"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1508
                     /locus_tag="VGB68_03080"
     CDS             <1..1508
                     /locus_tag="VGB68_03080"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0048243.1"
                     /translation="LTVVRDLRTGQVRDFHTDRSGRVWIATSSGGVLRIDEPDAEKPA
                     LINLTTAEGMASNQAGCLTEDDFGRIYAGTGRGVNRIEPETGRIKLFTKSDGLPENYI
                     RLCERDSSGALWFASLKGIARYVPQQDEQTPAPPIFLSDLRANGVSLKKLSELGESSV
                     GLPELASDQRQIQIEFFALGFGTGETLRYQYKLEGIDADWSQPSVQRTVNLGLSPGAY
                     NFLVRAVNSDGAASASPARASFTIARPIWQRWWFLIFAGLAASGVVYAIYGYRLRQLL
                     ELEKVRTRIATDLHDDIGASLSKIAILSEVVRQRVSPLAPKDAGISEPLEHIAGTSRE
                     MVDSMSDIVWAINPHKDRLSDLIQRMRNLAGEMTELCDIRLQVRLLGFEDRDLSLGAD
                     VRREIYLIFKETLNNLVKHSACSLAEIEFRLEENTLLITVRDDGKGFDSNGNGSGRGG
                     NGLPNMKRRAASLGGSYEIASQTGAGTTAVLIIPLFGGLRKINFGKLWSKK"
     gene            1607..2269
                     /locus_tag="VGB68_03085"
     CDS             1607..2269
                     /locus_tag="VGB68_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0048244.1"
                     /translation="MENLTAAPTVTKVVIIEDLRDIREGLATLLDFTEGFKCVGKFGS
                     MEEALRNIHRDVPDVVLSDIGLPGMDGIEGIGIIKEKYPEMTILMLSVYTDNERIFDA
                     LCNGATGYLLKKTPPARLIESLKEAVAGGAPMSPEIARQVITLFREVRPPERVEYDLT
                     PHETRLLKMLVDGHTKKTAAEEIGVSVNTVSFHLKRVYEKLQVHSKSEAVAKALRNRL
                     FK"
     gene            2479..>3130
                     /locus_tag="VGB68_03090"
     CDS             2479..>3130
                     /locus_tag="VGB68_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048245.1"
                     /translation="MKRAFAINFIVLILLSVFVSSIDAQTTAFVYQGKLNDANLPANG
                     TYQFEFKLFSAASGGSQIAQTIVDLPATVTNGIFAVDLDFGAGAFDGAERYLEISVRQ
                     SGSSQPYTTLTPRQPITSTPYAVKSLKSEEAKTALTANDSLKLAGIPASEYVQTTDTR
                     MSDARAPLPDSPYYIRNSQSQQTSSNFNISGEGKANVFNATGQFNLNGSRILSGNAN"
BASE COUNT          736 a          836 c          892 g          666 t
ORIGIN      
        1 atttgacggt tgtccgcgat ttgcgcaccg ggcaggtgcg ggattttcac accgaccgca
       61 gcgggcgcgt ctggattgcg accagctcgg gcggcgtcct cagaatcgac gagccggacg
      121 ccgaaaaacc cgcgttgatt aatctgacta ccgccgaggg aatggcgagc aatcaggcgg
      181 gctgcctgac cgaagatgat ttcgggcgca tctatgccgg gacggggcgc ggcgtcaatc
      241 gcatcgagcc ggagacggga cgcatcaagc tttttaccaa atccgacggt ttgccggaaa
      301 attatatcag gctttgtgag cgcgactcga gcggcgcgct gtggtttgcc tcgctcaaag
      361 gcatcgcgcg ttatgtcccg cagcaggacg aacagacgcc cgcgccgccg atttttctgt
      421 cggatttacg cgccaacggc gtttcgctta aaaagctttc cgagcttggc gaatcgagcg
      481 tcggcttgcc ggagctcgcc tccgaccaga ggcaaattca gatagaattt ttcgcgctcg
      541 gtttcgggac gggcgaaact ttgcgttatc aatataaact ggaaggcatc gacgccgact
      601 ggagccagcc gagcgttcag cgcacggtca atctcggtct gtcgccgggc gcatacaatt
      661 ttctggttcg cgccgttaac tcggacgggg cggcgagcgc gagtccggcg cgggcatcgt
      721 ttacgattgc ccgcccgatt tggcagcgct ggtggtttct gattttcgcc gggctggcgg
      781 cgtccggcgt cgtttacgcg atttacggct atcgcctgcg acagcttctg gaactggaaa
      841 aagtccgcac gcgcatcgct accgatttgc acgacgacat cggcgcttcg ctttccaaaa
      901 tcgctatcct gagcgaagtc gtccgccagc gcgtttcgcc gctcgcgccc aaagatgccg
      961 gaatcagcga gccgctcgaa cacatcgccg gaacttcgcg cgagatggtc gattcgatgt
     1021 cggacatcgt ctgggcgatt aacccgcata aagaccgcct cagcgatttg attcagcgaa
     1081 tgcgcaatct ggcgggcgaa atgaccgagc tttgcgatat tcgcctgcaa gtccgattgc
     1141 tcggcttcga agacagggat ttgtcgctcg gcgcggatgt gcggcgcgag atttatctga
     1201 tttttaagga aacgctcaac aatctggtca agcattcggc ttgcagcctg gcggaaatcg
     1261 aattccggct cgaagaaaat actctcctca ttacggtcag agacgacgga aaaggtttcg
     1321 acagcaatgg aaacggcagc ggtcgcggcg gcaacggctt gccgaatatg aaaagacgcg
     1381 ccgcgagcct cggcggaagt tatgaaatcg cttctcaaac cggcgcgggc acgacggctg
     1441 ttttgataat tccgcttttc ggcggactgc gaaaaataaa tttcgggaaa ttatggtcga
     1501 aaaaataaac cgctacccga aggtgtagtg acgagccgtc attgaaaaca taaactgtag
     1561 ttgaaggaaa aagtttatgt tacaggaaat acaaagccga gacgttatgg aaaatttaac
     1621 cgccgcgccg accgtcacca aagtcgtcat catcgaagat ttgcgcgaca tccgcgaagg
     1681 actggcgacg cttctggatt ttaccgaagg ctttaaatgc gtcggcaaat tcggttcgat
     1741 ggaagaagcc ctgcgaaata ttcaccgcga cgtgccggac gtggttttgt ccgacatcgg
     1801 gctgccgggt atggacggca tcgaaggcat cggcattatc aaggaaaaat atccggaaat
     1861 gacgattctg atgctctcgg tttacaccga caacgagcgg attttcgacg ccttgtgcaa
     1921 cggcgcgacc ggctatctgt taaagaaaac gccgcccgcg cgcttgattg aaagcttaaa
     1981 ggaagccgtc gcgggcggcg cgccgatgtc gccggagatt gcgcgccagg tcatcacgct
     2041 gtttcgcgaa gtgcgaccgc cggagcgcgt cgaatacgat ttgacgccgc acgaaacccg
     2101 gcttttaaaa atgctggtcg acggtcacac caagaaaacc gccgccgaag aaatcggcgt
     2161 ctccgtcaac acggtttcgt ttcacctgaa gcgcgtttac gagaaattgc aggttcattc
     2221 aaaatcggaa gcggtggcga aggctttaag aaaccgtctg tttaaataaa aatcgaaaaa
     2281 gcgcgaacct cgcagagcct gtagaattgt tagcggcaaa atcgagtcat cgaacagctt
     2341 gccgcttttt tattttttcg caaaagacga aaacttccct acacgttcgt gtggtgaccg
     2401 ggagattcgt cttcgattat tcttcgttcg tcgattaaag cgccgttttt gcgcagtcgg
     2461 gaaatgtgga gaataattat gaaaagagca ttcgcaatca attttatcgt cttaatttta
     2521 ctatcggttt ttgttagctc gattgatgcc cagacgacgg ctttcgtcta tcagggcaaa
     2581 ctgaacgacg caaatctgcc ggcgaacggg acttaccagt tcgagttcaa attattcagc
     2641 gcggcgagcg gcggcagtca aatcgcccag accatcgtcg atttgccggc gacggtgacc
     2701 aacgggattt tcgccgtcga tttggacttc ggcgcaggcg cgttcgacgg cgcggagcgt
     2761 tatctggaaa tcagcgtgcg ccagagcggg agcagccaac cctacacgac gctcacgccg
     2821 cgccagccga ttacctcgac gccttacgcc gtaaaaagtt taaaatccga agaagcgaag
     2881 acggcgctga cggcgaacga ttccctgaag ctggcaggca ttccggcgtc ggaatacgtt
     2941 cagacgaccg ataccagaat gtcggacgcg cgcgcgccgc tgccggacag cccttattac
     3001 atccggaatt cgcagagcca acagacgtct tccaatttca acatttccgg cgaaggcaag
     3061 gcaaacgtct ttaatgcgac cgggcagttc aatcttaacg gcagccggat tttgagcggc
     3121 aacgccaacg
//
LOCUS       DASWNE010000152         9176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_357247, whole genome shotgun sequence.
ACCESSION   DASWNE010000152 DASWNE010000000
VERSION     DASWNE010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9176
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357247"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VGB68_03095"
     CDS             complement(<1..323)
                     /locus_tag="VGB68_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HEY0048246.1"
                     /translation="MNKIIVLIGAPGAGKGTQARLLEERRGIPQISTGDMFRALKNAD
                     TPLAREVQLIMKEGKLISDHITFEIVKDRTAREGGSYILDGYPRTAVQARDLENLAEA
                     QGRKI"
     gene            complement(439..1857)
                     /gene="secY"
                     /locus_tag="VGB68_03100"
     CDS             complement(439..1857)
                     /gene="secY"
                     /locus_tag="VGB68_03100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEY0048247.1"
                     /translation="MDKFFAALQNMFKVPELRSRILFTLGMLVVYRFGAHIQAPGINK
                     AQLERVWSDVSGTLLGVLDLFSGGNFRTISVFALGVTPYITASIIIQLMTVLYPALKK
                     VQEEGEVGRQKLNQWTRYLTVILCAVQTFFVATWLSRNQIIGETWWATFMIVVTLTTG
                     TVFVMWLGEQITERGIGNGISLLIFAGIVIGLPSGLMQISERVRGGNPMQTFGVIFLG
                     VVLIALIALIVYVESARRNIAISYASRRVGNQTFKGQETVLPLKINMGGVIPVIFASS
                     VLAMPQTLLSAFPPDPNAPESTWARVFQFFQAFHGGDPYYELVFLSLIVLFTFFYITI
                     VFNTDEVADNLRKHGGFIPGIRPGAQTAEYLNYILTRLTTVGALYLAFVAFAPQVLLS
                     GFKVGRLPFIGPSVDQFFAATPGLTWIPNGLGYQFFFGGTSLLILVGVAMDTISQIEA
                     QLVMRNYEGFLGAGSRLRGRRG"
     gene            complement(1933..2412)
                     /gene="rplO"
                     /locus_tag="VGB68_03105"
     CDS             complement(1933..2412)
                     /gene="rplO"
                     /locus_tag="VGB68_03105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEY0048248.1"
                     /translation="MALTLNNLHPAPGSTHKKKRVGRGPGSGLGKTSGRGHKGQKSRS
                     GYSSKSGFEGGQMPLQRRLPKRGFTNIFKKQWLEISLAKLEENFNAGDEITPEILHQR
                     GLIKKAKHDLVILGTGDISKALKISAHRFTKNAKDKIEKAGGSITEIVKEKAADAQA"
     gene            complement(2518..2724)
                     /gene="rpmD"
                     /locus_tag="VGB68_03110"
     CDS             complement(2518..2724)
                     /gene="rpmD"
                     /locus_tag="VGB68_03110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEY0048249.1"
                     /translation="MANKTEQENSGGSIKIQYYRSSIGYSGKQKAIVKSLGITKLNQT
                     VERPDTPSMRGIVAKVPHLLRIVE"
     gene            complement(2811..3320)
                     /gene="rpsE"
                     /locus_tag="VGB68_03115"
     CDS             complement(2811..3320)
                     /gene="rpsE"
                     /locus_tag="VGB68_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016196837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEY0048250.1"
                     /translation="MKTNNQRISPQGLILKDQLISINRVTKVVKGGKNMSFAALVVVG
                     DEDGHVGFGTGKAKEVPNAIKKAVEAAKKNLIKVTLINGTLPHPIIGKYGAGQVLLKP
                     AEDGTGVIAGGAVRAVLQSLGVHNVRTKILGSNNAHNVIRATFDGLTRMKDPMEVARL
                     RGKQVEELL"
     gene            complement(3307..3720)
                     /gene="rplR"
                     /locus_tag="VGB68_03120"
     CDS             complement(3307..3720)
                     /gene="rplR"
                     /locus_tag="VGB68_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEY0048251.1"
                     /translation="MANKDRAGIRRAVHSRIRKKVRGTPERPRLAVFRSVNHIYAQVI
                     DDINGKTLAAASSVEKDLKGSTGGNVEAAQRIGRAVAERALAAGVSNVVFDRGGYVYH
                     GRVKALLDATREAGLNKNETEAQETGVTEEANENK"
     gene            complement(3779..4339)
                     /gene="rplF"
                     /locus_tag="VGB68_03125"
     CDS             complement(3779..4339)
                     /gene="rplF"
                     /locus_tag="VGB68_03125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEY0048252.1"
                     /translation="MSRVGRKPITIPSGVTVTIQDSELEVKGPKGTLKTPIPNGINFK
                     IEDNTLFAERQNDDQAAFHGLGRALANNAIVGVTEGFKREMDVVGVGYKADVQGKKIV
                     FALGYSHPIEYPLPDGIEAKAERVPTKTTINQYQLTLTLTGIDKQKLGQVAAELNRLR
                     KPDAYKGKGVRYADRTYKLKPGKTGK"
     gene            complement(4449..4841)
                     /gene="rpsH"
                     /locus_tag="VGB68_03130"
     CDS             complement(4449..4841)
                     /gene="rpsH"
                     /locus_tag="VGB68_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEY0048253.1"
                     /translation="MTDPIADMLTRIRNAIQAKHSRVDIPGSKLKMEIARILKEEGYI
                     NNYVTKGEGVKFAIRIFLRYDAKGASSITHLNRVSRPSRRVYVGATEIPRVLGGYGVN
                     IISTSKGLMSGKRARRENMGGEVLAEIY"
     gene            complement(4920..5225)
                     /gene="rpsN"
                     /locus_tag="VGB68_03135"
     CDS             complement(4920..5225)
                     /gene="rpsN"
                     /locus_tag="VGB68_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEY0048254.1"
                     /translation="MAKISKVVKNEKRKKLVTQYAERRAELKKIINNPTSTPEQVDEA
                     VTRLQKLPRDASPIRVRNRCSMTGRPRGYLRKFGASRVALRELALRGQIPGVVKSSW"
     gene            complement(5305..5844)
                     /gene="rplE"
                     /locus_tag="VGB68_03140"
     CDS             complement(5305..5844)
                     /gene="rplE"
                     /locus_tag="VGB68_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEY0048255.1"
                     /translation="MARLKEKYRNEIAPALAKEFGIENPMAIPRIEKIVVNMGLGEAI
                     ANAKILDTAVEELRSITGQKPVVTKAKKSIASFKLRQGMNIGTMVTLRGNRMYEFLDR
                     LISVGLPRVRDFRGVSGKAFDGRGNYTLGIREQLIFPEIDFNKVDKTRGMNISIVTTA
                     ENDEQARALLKAMGMPFRQ"
     gene            complement(5902..6339)
                     /gene="rplX"
                     /locus_tag="VGB68_03145"
     CDS             complement(5902..6339)
                     /gene="rplX"
                     /locus_tag="VGB68_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEY0048256.1"
                     /translation="MKEVKKGTIRSKIKRGDTVVFISGREFNRYEKSTKGGQEEVKRV
                     PLRGEVIEVDPRKGKVKVGGAQIMKRHRKAVPQMNVEGGIIESEGWVDISNVAVVDPK
                     TGKPTRVKYEMRDGKKVRVAKSGELIPDPPRRTNTKKKDNDNE"
     gene            complement(6343..6711)
                     /gene="rplN"
                     /locus_tag="VGB68_03150"
     CDS             complement(6343..6711)
                     /gene="rplN"
                     /locus_tag="VGB68_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEY0048257.1"
                     /translation="MIQMQTSLVVADNSGAKRVEMIMAIGGSTGSTATIGDKIKVTVK
                     EAAPDGTAKKGKVYNAVVVRTRKEIRRKDGTYIRFDENAAVLIKDDGSPIGTRVFGPV
                     ARELRDKNFMKIVSLAPEVI"
     gene            complement(6818..7039)
                     /gene="rpsQ"
                     /locus_tag="VGB68_03155"
     CDS             complement(6818..7039)
                     /gene="rpsQ"
                     /locus_tag="VGB68_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEY0048258.1"
                     /translation="MVTSDKMTKTVVVRVDRLVKHPKYRKYVRRRKQFMAHDELGATI
                     GDKVRIIETRPLSARKRWRVVEIVQKAAK"
     gene            complement(7449..7661)
                     /gene="rpmC"
                     /locus_tag="VGB68_03160"
     CDS             complement(7449..7661)
                     /gene="rpmC"
                     /locus_tag="VGB68_03160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEY0048259.1"
                     /translation="MKRKEELEQLHGMSVEELKEQADSLKESLFRLKFRKTLGVGESV
                     SAIKREKKTLARVNTLIRRMETKSNS"
     gene            complement(7661..8089)
                     /gene="rplP"
                     /locus_tag="VGB68_03165"
     CDS             complement(7661..8089)
                     /gene="rplP"
                     /locus_tag="VGB68_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEY0048260.1"
                     /translation="MLMPKKVKHRRVMKGRNRGVASRGDKLNFGDFALKALEAGWITD
                     RQIEAARISMTRHIKRGGKVWIRMFPDKPITKKPAETRMGSGKGAPDHWVAVVKPGRV
                     LYEIQGVTEELAREAMNLAAQKLPIKVKFVTRAELEGGIV"
     gene            complement(8173..8919)
                     /gene="rpsC"
                     /locus_tag="VGB68_03170"
     CDS             complement(8173..8919)
                     /gene="rpsC"
                     /locus_tag="VGB68_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016654883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEY0048261.1"
                     /translation="MGQKVHPYGFRVGYNKDWHSHWFAKKEYAEFLAEDMKLKRELKK
                     RFMGAGVSHIDVERAANRLKIIIYTSRPGIIIGRKGTEIEKLKEDLSKKTGREVIISI
                     QEVQKPELNAQLQAEKIAQQLEKRIAFRRAMRKILEESQRFGAEGVKVMCAGRLNGAE
                     IARTEWSLEGRLPLHTLRADIDYGFAEALTTFGIIGIKVWIYRGEILDPNASMARGTT
                     TDPMKEVKVKDQGRGRGRGDRPPRRDRNDR"
     gene            complement(8932..>9176)
                     /locus_tag="VGB68_03175"
     CDS             complement(8932..>9176)
                     /locus_tag="VGB68_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048262.1"
                     /translation="ETTTATTTEQTAGETTTAAATGETSPKATESSKAVTEEETEGDA
                     AASDAKDTATQFPAELNETKRADDKQLDVNEQLERQ"
BASE COUNT         1850 a         2534 c         2215 g         2577 t
ORIGIN      
        1 tgaatctttc tgccctgcgc ctcagccaga ttttcgaggt cgcgcgcctg caccgccgtg
       61 cgcgggtaac cgtccagaat gtagctgccg ccctcgcgcg cggttctgtc cttaacgatt
      121 tcaaaagtaa tgtggtcgga aattaatttt ccttccttca ttatcaactg cacttcgcgg
      181 gcgagcggcg tgtcggcgtt tttcagcgcg cgaaacatat cgccggtcga gatttgcgga
      241 atgcctcggc gctcttccag aagcctcgcc tgcgttccct tgcccgcgcc gggcgcgccg
      301 attaaaacga taattttgtt cattcaaata tatttttcac cgcgcaggca ccgcagacgc
      361 agagttttcg gaagtaaaaa gaaaaaattt cttttccggc tttctgcgtt tgccgcgtct
      421 agtgcggtga aaaatttcct aacctctacg accgcgcaga cgcgaacccg cgcctaaaaa
      481 gccttcgtag tttctcatca cgagctgcgc ttcgatttgc gagatggtat ccatagccac
      541 gccgaccaga atcagaagcg aagtgccgcc gaaaaagaac tggtagccca agccgttcgg
      601 aatccaggtc aagccgggcg ttgccgcgaa aaactggtcg acgctcggac cgataaacgg
      661 cagacgtccg actttaaacc cgctcagcag cacctgcgga gcaaaagcga caaacgccag
      721 gtaaagcgct ccgaccgtcg tcaaacgcgt cagaatataa ttcagatatt cagccgtctg
      781 cgcgcccgga cgaatgcccg gaataaatcc gccgtgtttg cgaagattgt ccgccacctc
      841 gtccgtatta aacacaatcg ttatatagaa aaacgtgaac aggacaatca gcgaaagaaa
      901 gacgagttca taatacgggt cgccgccgtg aaacgcctga aagaattgaa aaactctcgc
      961 ccaggtcgat tccggcgcgt tcgggtcggg cggaaaagcc gacaaaagcg tctgcggcat
     1021 cgccagcacg gacgaagcga aaatcacggg aatgacgccg cccatattga ttttcagcgg
     1081 caggacggtt tcctgtccct taaacgtctg atttccgacc cggcggctgg catagctgat
     1141 ggctatgttt cgacgcgccg attcgacgta aacaatcagc gcgatgagcg caatcaaaac
     1201 cacgccgagg aaaatcacgc cgaacgtctg catcgggttg ccgcctctga cgcgctcgga
     1261 aatctgcatc agtccgctcg gcaaaccgat aacgatgccc gcgaaaataa gcagcgagat
     1321 tccgtttccg atgccgcgct cggtgatttg ttcgccgagc cacatcacga aaaccgtgcc
     1381 ggtcgtcaag gtcacgacaa tcataaacgt cgcccaccag gtctcgccga taatctggtt
     1441 gcggctgagc caggtggcga caaaaaaggt ctgcacggcg cacaaaataa cggtcagata
     1501 acgcgtccac tggttgagtt tctgacgccc gacctcgcct tcttcctgca cttttttcag
     1561 cgccggataa agaaccgtca tcagctgtat gataatcgac gccgtaatat acggcgtgac
     1621 gccgagcgcg aaaacggaaa tcgtgcggaa attgccgccc gaaaaaaggt cgagcacgcc
     1681 gagcagagtt ccgctgacgt cgctccagac tctttcaagc tgagctttat taattcccgg
     1741 agcctgaata tgcgcgccga aacgataaac aaccagcatt ccaagcgtga aaagtatccg
     1801 gcttctcagt tccggcactt taaacatatt ttggagcgcg gcaaaaaact tatccattat
     1861 attcctctag aacaaaaatt tcttccgagt cagtttagcg tgttttcgac tgacaaaaaa
     1921 gccgcgcgag gcttaagcct gcgcgtcggc ggctttttcc ttaacgattt ccgttatgct
     1981 gccgccggct ttttcgattt tgtctttcgc gtttttcgta aagcggtgcg ccgaaatttt
     2041 caaggctttg ctgatgtcgc cggttcccag gataaccaaa tcgtgcttgg cttttttaat
     2101 cagcccgcgc tggtgcagta tttccggcgt gatttcgtcg ccggcgttaa agttttcttc
     2161 gagcttggcg aggctgattt cgagccattg ctttttgaaa atattggtga aaccgcgttt
     2221 cggcaaacgg cgctgcaaag gcatctgtcc gccttcaaaa ccggatttgc tcgaatagcc
     2281 cgaacgcgat ttttgtcctt tatgaccgcg tccggaagtt tttcccaaac ccgaacccgg
     2341 accgcgcccg actcttttct tcttgtgcgt gcttcccggc gcaggatgca agttgtttaa
     2401 tgttaatgcc attttctttt cctcttaaaa aggatttcac cataaaggca caaagaacac
     2461 aaaggctttg acgatgcttt tccttgtgtc ctttgcgcgt ttgtggttaa tcccaatcta
     2521 ttcgacaatt ctcaaaagat gcggaacttt ggcgacaatt ccgcgcatcg aaggcgtgtc
     2581 ggggcgctca accgtctgat tgagtttcgt gatgcccaga cttttgacaa tcgctttctg
     2641 tttgccggaa tagccgattg agcttcgata atactgaatc ttgatgcttc cgccgctatt
     2701 ttcttgttct gttttgtttg ccattacttt atcgtttgag caaccagcta tcagctatca
     2761 gctatcagct tttttctttg ctgaccgctg acggctgatg gctgatggct ttataaaagt
     2821 tcttccacct gtttgccgcg caggcgcgcg acttccatcg ggtctttcat tcgggtcaag
     2881 ccgtcgaaag tggcgcgaat cacgttgtgc gcgttgttcg agcctaaaat cttcgttctc
     2941 acgttatgga cgccgagcga ttgcaaaacc gcgcgcaccg cgccgcccgc gatgacgccc
     3001 gttccgtctt ccgccggttt gagcaaaacc tgtcccgcgc cgtattttcc gataatcggg
     3061 tgcggcaagg ttccgttaat caacgtcact ttaatcagat ttttctttgc cgcctcgacc
     3121 gcttttttaa tcgcgttcgg aacttctttc gcttttccgg tgccgaaacc gacgtgaccg
     3181 tcttcgtcgc cgacgacgac cagcgcggca aatgacatat ttttaccgcc tttgacgacc
     3241 tttgtaacgc gattaatcga aatcaactgg tcttttaaaa tcagcccctg cggcgagatg
     3301 cgttggttat ttgttttcat ttgcttcttc cgttactcca gtttcctggg cttccgtttc
     3361 gtttttgttc aagcccgctt cgcgcgtcgc gtcgagcaga gctttgacgc gaccgtgata
     3421 gacataaccg ccgcggtcaa aaaccacgtt cgatactccg gcggcaagcg cgcgctcggc
     3481 aaccgctctg ccgattctct gcgcggcttc gacgtttccg cccgtcgagc ctttcaaatc
     3541 tttttcgacg ctcgaagcgg cggctaaagt ttttccgttt atatcgtcaa tcacctgcgc
     3601 gtaaatatga tttacgctgc ggaaaaccgc cagccgcggg cgttccggcg ttccgcgcac
     3661 ttttttacga atgcggctgt gaacggcgcg acgaattcca gctctatctt tgtttgccat
     3721 atcttaacct ttattttgat ggactgctcg cccgttttgc ggcggcaggt tacatctatt
     3781 atttgccggt tttgccgggt ttcagtttat aagttctatc ggcgtagcga acgcctttgc
     3841 ctttgtaagc gtccggtttg cgcaggcgat tcaattcggc ggcgacctga ccgagctttt
     3901 gtttatcgat gccggtcagc gtcagcgtca gctgatattg attaatcgtc gtttttgtcg
     3961 gaactctctc ggctttcgct tcgatgccgt ccggcaaagg atactcgatc gggtgcgagt
     4021 agccgagcgc gaaaacgatt ttcttgccct gcacgtccgc cttgtagccg acgccgacga
     4081 cgtccatttc ccttttaaag ccttcggtga cgccgacaat cgcgttatta gccagcgccc
     4141 gacccaagcc gtgaaatgca gcctggtcgt cattctggcg ttcggcgaat aatgtattgt
     4201 cttcgatttt aaaattgatg ccgttcggaa tcggagtttt caaagtccct ttcggacctt
     4261 tcacttccag ttcgctgtcc tgaatcgtca cggtcacgcc cgacggtatc gtaataggtt
     4321 ttcttccaac acgagacata atatcctctt ttgattttag attggcgacg ccggataagt
     4381 gttttttaat ttatcgcatc cggctcaaag ccgaattccg ctttcaatct aaaatctaaa
     4441 atctaaaatt aatagatttc cgctaaaact tcgccgccca tattttcgcg gcgcgctctt
     4501 tttccactca tcaagccttt cgaggtggaa ataatattta ctccgtaacc gccgagcacg
     4561 cgcgggattt cggtcgcgcc gacgtaaacg cggcggctcg ggcgtgaaac gcgattcaga
     4621 tgcgtgattg aagaagcgcc tttcgcgtcg tagcgcagga aaatgcggat ggcgaatttg
     4681 acgccttcgc ctttcgtgac gtagttgttg atgtagcctt cttctttcag gatgcgggcg
     4741 atttccattt tcagtttcga accgggaata tcgacgcgcg agtgttttgc ctgtatcgca
     4801 ttacggattc gcgtaagcat atcggcgatt gggtctgtca ttttaaatct actccgtata
     4861 aaaattacga tggttcgcaa atgcctgatt gcgcctgcgc ctgtcgtagt ctagaaaaat
     4921 taccagcttg atttcaccac gcccggaatc tgcccgcgca gggctaattc acgaagcgcg
     4981 acgcgcgagg cgccgaattt gcgcaggtat ccgcgcgggc gtccggtcat cgagcagcga
     5041 ttgcgaaccc gtatcgggct ggcatcgcgc ggcagttttt gcagccgcgt caccgcttcg
     5101 tcgacctgtt ccggcgtcga agtcggattg ttgatgattt ttttcagttc cgcgcgacgc
     5161 tcggcgtatt gtgtcaccag ctttttgcgt ttttcgtttt taacgacttt gctaatcttt
     5221 gccatatata aaaatttgag attcaggatt caggattcaa gattcaagat taccaatcct
     5281 gaatcttgaa tcctgaatct ttaattattg tctaaacggc attcccatcg ctttcagaag
     5341 cgcgcgcgct tgttcatcgt tttctgccgt cgtcacgatt gaaatattca ttccgcgtgt
     5401 tttatcgact ttgttaaagt cgatttccgg aaaaatcaac tgctcgcgaa tgccgagcgt
     5461 atagtttccg cgcccgtcaa acgccttgcc ggaaacgccg cgaaaatcgc ggacgcgcgg
     5521 cagaccgacc gaaatcaggc ggtcgagaaa ttcatacatt ctgtttccgc gcaaggtgac
     5581 catcgtgccg atgttcattc cctggcgcag cttaaacgaa gcgattgatt ttttcgcttt
     5641 cgtcacgacc ggtttttgtc cggtaatcga gcgcagctct tcgaccgccg tgtccagaat
     5701 cttggcgttg gcgatagctt cgccgagtcc catattgacg acgatctttt cgatgcgcgg
     5761 aatcgccatc ggattttcga taccgaactc tttggcgagc gccggagcga tttcgttacg
     5821 atatttttct tttaatcttg ccattgtttt attttagcgt ttgggtctcg cgcgtttcgg
     5881 gcgtttccaa actttcaaaa attattcgtt atcgttatct ttctttttcg tattggtccg
     5941 gcgcggcgga tcgggaatca attctccgct ttttgccacg cggacttttt tgccgtcgcg
     6001 catttcgtat ttgacgcgcg tcggttttcc ggttttcggg tcgaccacgg cgacgtttga
     6061 aatatccacc cagccttccg attcgatgat tccgccttcg acgttcatct gcggcacggc
     6121 tttacggtgg cgcttcataa tctgcgcgcc tccgaccttg actttgccct tgcgcgggtc
     6181 aacttcgatg acttcgccgc gaagcggaac gcgcttgact tcttcctgac cgcctttcgt
     6241 cgacttttcg tagcggttaa attctctgcc cgagataaaa acaaccgtgt cgccgcgttt
     6301 gatttttgag cgaattgttc cttttttaac ttccttcatt gtttaaatca cctccggcgc
     6361 caaggatacg attttcataa aattcttgtc gcgcaattct ctggcaaccg gaccgaaaac
     6421 gcgcgtcccg atcggcgagc cgtcgtcttt gataagcacg gcggcgtttt cgtcaaagcg
     6481 gatgtatgtc ccgtccttgc ggcggatttc cttacgcgtt ctgacgacga cggcgttgta
     6541 aactttacct tttttcgccg ttccgtccgg cgcggcttct ttgacggtga ctttgatttt
     6601 atcgccgatc gtcgcggtcg agccggtcga gccgccaatc gccataatca tttccacccg
     6661 tttcgcacct gaattgtctg cgaccactaa cgaggtctgc atctgaatca taactgtttc
     6721 actccgttca aagttgactg agttgactga gttgactgag ttgactgaag ttaaagaaac
     6781 tcagtcaact tcggcaaact cgctaaactc ggtgaactta ttttgctgct ttctgcacaa
     6841 tttccacgac gcgccagcgt ttacgcgcgg aaagcggtct ggtttcgatg attcgaacct
     6901 tgtcgccgat ggtcgcgccg agctcgtcgt gcgccataaa ctgttttcgg cggcgcacgt
     6961 atttgcgata tttcggatgt ttgaccaggc ggtcaacgcg aacgacgacc gttttcgtca
     7021 tcttgtccga ggtaaccata ccgattctct cggagcgttt gccgtgacca cccgtttccg
     7081 ttttattatc gtccgcgccg gtcacaaaat tagtaaccgt gctcgcgacg gtttccaccg
     7141 ccgaagcgag cgtcccgact acagcgtcca gaattgaact tccctgcgtt tcgccttcgg
     7201 atgcagcttc gttggatttc ttcggctcct cttcggttgc ctcggtgttt tccgtctggg
     7261 ctgcttcggc gtcgccggct gcctgcgttt cgaccgctcc ggaattttct tccgccgccg
     7321 ctgctgctgt ctcttcggtc gcttccgcgc cgttttcaac tgtttcttcg tctttctttc
     7381 ttggcatttc tttttccagt taatcgtgaa ccgtgaatcg caaatagaaa agctttacta
     7441 ttaacgattt acgaattact tttcgtttcc atccggcgaa tcaacgtatt gaccctcgcc
     7501 agagttttct tttcacgttt gatagcgctg accgattcgc cgacgcccag tgtttttctg
     7561 aacttcaggc gaaagagcga ttcttttaag ctgtcggctt gttcttttaa ttcttcgacc
     7621 gacattccgt gaagctgttc taattcttct ttgcgtttca ttagactatg cctccttcca
     7681 gttcggcgcg cgtgacaaac ttcactttaa tcggaagctt ctgcgccgcc agattcattg
     7741 cctcgcgcgc caattcttcc gtgacgccct gaatttcata taaaacgcgt cccggcttga
     7801 cgaccgcgac ccagtggtcg ggcgcgcctt tgccgcttcc catacgcgtt tcagccggtt
     7861 ttttcgtaat cggcttgtcg ggaaacattc gaatccagac ttttccgccg cgcttgatat
     7921 gacgcgtcat cgagatacga gccgcttcaa tctggcggtc ggtaatccag cccgcttcca
     7981 gcgctttcaa tgcaaagtca ccgaagttta atttgtcacc gcgcgaagcg acgccgcgat
     8041 tgcgaccttt cataaccctg cggtgcttga cctttttcgg catcaacata atgagttacc
     8101 tctaaatatt cgcgatttcg gacgaggaaa tcagagttcc attcaaaatc caaaatctaa
     8161 aatctaaaat tcttatctgt cgttacgatc acgacgcggc ggacggtcac cgcgaccgcg
     8221 tccgcgaccc tggtctttta ctttcacttc tttcatcggg tcggtcgtcg ttccgcgcgc
     8281 catcgaagcg ttcgggtcga gaatctcgcc gcgataaatc cagaccttga ttccgataat
     8341 tccgaaagtc gtcagggctt cggcaaaccc gtaatcaatg tcggcgcgaa gcgtatgaag
     8401 cggcaatctg ccttccagac tccattcggt gcgggcgatt tccgcgccgt ttaagcgtcc
     8461 ggcgcacata actttcacgc cttcggctcc gaagcgctgc gattcttcca gaatctttct
     8521 catcgcgcgg cgaaacgcga tacgtttttc gagctgctgc gcgatttttt ccgcctgcag
     8581 ctgcgcgttg agctcaggtt tctggacttc ctgaatcgag ataatcactt cgcgtccggt
     8641 ttttttcgac aaatcttcct tgagcttttc gatttccgta cctttgcgtc cgatgataat
     8701 gccggggcgc gaggtgtaaa tgataatttt caaacgattg gcggcgcgct cgacgtcaat
     8761 atgcgagacg cctgctccca taaagcgctt tttcagctcg cgcttcaatt tcatatcttc
     8821 cgccaggaat tcggcgtatt cttttttggc aaaccagtgt gaatgccaat ctttgttgta
     8881 tccgacccgg aagccgtatg gatgaacttt ttgtcccata atataaatct cttactgtct
     8941 ttccagttgt tcgttgacgt ccagttgctt atcgtcagcc cgtttggttt cgtttaattc
     9001 tgcgggaaac tgcgtcgccg tatctttcgc atccgacgcc gcggcatcgc cttcggtttc
     9061 ttcttcggtg acggctttcg agctttccgt cgctttcggc gaagtttctc cggttgccgc
     9121 cgccgtcgtt gtttctccgg cggtttgttc ggtcgtcgtc gccgtcgtcg tttcga
//
LOCUS       DASWNE010000153         2355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_145896, whole genome shotgun sequence.
ACCESSION   DASWNE010000153 DASWNE010000000
VERSION     DASWNE010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145896"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1597
                     /locus_tag="VGB68_03180"
     CDS             <1..1597
                     /locus_tag="VGB68_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048263.1"
                     /translation="ILPLRLCVSAVLIFLIFTACQSAKPTDMRSLVPAETLVYMESRD
                     LAKTLSALTENKAWQELAKSKPDFSYLENTQLAVAVTGFETSEKQLTDEQAVLNFKPR
                     FVAVADTHRWESTNLSLTENQIGKFARATYGDDAKLEKAEKSGAKWFVWTAADNRKLF
                     AAVSQSVIYLGNDEAAIEKCLAVKRGETENLTKNESLTRTTERANDENQIALGYISPE
                     SMPQIANLASVFAALRVSEDADARSFTAQILPQILQKSVREILWTARKTERGIEDKYL
                     IKTSAEISSVFKETLLPASAREFQKAEFLPPALFSVTRYNLQNPQIAWRSVLLATANQ
                     TDAGSAKIILQFSDALFEPYGIERAETFLSAAGAEIITAKFDEEGEKSVVIADIKDAE
                     SLKKALTGEMDFKTPPEKQGNAEIWKSKDKLSAAAFVENKLILGETESVLSCLKARES
                     GQNFKQTEAFQKISTSPAAAVSLTKDAETAGKIVEALGNPKEENKTSASFYTTETRFD
                     ATGFERKTVSDFGLLGMILESLE"
     gene            complement(1731..>2355)
                     /locus_tag="VGB68_03185"
     CDS             complement(1731..>2355)
                     /locus_tag="VGB68_03185"
                     /inference="COORDINATES: protein motif:HMM:NF021759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HEY0048264.1"
                     /translation="GKVFVRIAQIFEAAPLEVQKSLASILVSKILRRRAPRADSENYQ
                     AFVRTESIQAQAFENRRRRGRKIVTTSKGEVYDLEEIFDRLNRIYFQNRLPKPVLTWS
                     AQKTFHRLGHHDSTHETLVISKSLDDKKVPAFVTEYVVYHEMLHIKHPTILRNGRRYN
                     HTPAFRRDEEKFAYFAEADTWIERNARALKRKVKTQRRRKRPSLSGR"
BASE COUNT          608 a          583 c          634 g          530 t
ORIGIN      
        1 aattcttcct ttgcgccttt gcgtctccgc ggttttaatc ttccttattt ttacggcttg
       61 tcaatccgcg aagccgaccg atatgcgcag cctcgtgccc gccgaaaccc tggtttatat
      121 ggaatcgagg gatttggcga aaactctttc ggctttgacc gaaaacaaag cgtggcagga
      181 actggcgaaa tcgaaacccg atttttcata tctggaaaac acgcagctcg ccgtcgccgt
      241 gacgggtttt gaaacttccg aaaaacaatt gaccgacgag caggcggttt tgaatttcaa
      301 gccgcgtttt gtcgccgtcg ccgatacgca ccgttgggaa tcgacgaatt tatcgctgac
      361 cgaaaaccaa atcggaaaat tcgcgcgcgc gacttacggc gacgacgcaa agctggaaaa
      421 agcggaaaag tcgggcgcca aatggtttgt ctggacggcg gcggacaatc gaaagctttt
      481 cgccgccgtt agtcagagcg taatttatct cggcaacgac gaagcggcga ttgaaaaatg
      541 cctggcggtc aaacgcggcg agacggaaaa tttaacgaaa aatgaatcgt tgacgcggac
      601 gacggaacgc gcaaacgatg aaaatcaaat cgcgctcggt tatatttcgc ccgaatcgat
      661 gccgcagatt gcgaatctgg cgagcgtttt cgcggctttg cgcgtgagcg aagacgcgga
      721 cgcgcgaagc tttacggcgc agattctgcc gcagattttg cagaaatccg tcagggaaat
      781 cctctggacg gcacggaaaa ccgaacgggg aatcgaagac aaatatttaa tcaaaaccag
      841 cgcggaaatt tcttccgttt tcaaggaaac gcttttgccc gcttccgcca gagaatttca
      901 aaaagccgaa tttctgccgc ccgcgctttt ttccgtgacg cgctacaatc tgcaaaatcc
      961 gcaaatcgcg tggcgcagcg ttttgctggc gacggcaaat cagacggacg cgggaagcgc
     1021 gaaaattatc ctgcagtttt ccgacgcgct gttcgagcct tacggaatcg agcgcgccga
     1081 aacgttttta agcgctgccg gcgcggaaat catcacggcg aagtttgacg aagaaggcga
     1141 aaaatccgtc gtgattgccg acatcaaaga cgccgaaagc ttgaaaaaag cgctgaccgg
     1201 cgagatggat ttcaaaacgc cgccggaaaa acagggaaac gccgagattt ggaaatccaa
     1261 agacaaactt tcggcggcgg ctttcgtcga aaataaattg attctgggcg agaccgaaag
     1321 cgttttaagt tgtctgaaag caagggaaag cgggcaaaat tttaagcaaa cggaagcttt
     1381 tcaaaaaatc tcgacgagtc cggcagccgc cgtcagcctg acgaaagacg cggaaactgc
     1441 cggaaaaatc gtcgaggctc tgggcaatcc gaaagaagaa aacaaaacgt ccgcgagctt
     1501 ttacacgacc gaaactcgct ttgacgcaac cggattcgag cgcaaaacag tttcggattt
     1561 cggtttgctc ggaatgattc tggaaagcct ggaatagtcc ggaaacgccc gtcgacggcg
     1621 ggcgggtttt ataattttag attttagatt tcgggtctgc cgttatcgaa cgaccgattt
     1681 tcaatctaaa atctaaaatc taaaatctaa aatctaaaat ctaaaatcga ttatctgccc
     1741 gacagcgacg ggcgtttccg gcggcgctgg gttttgactt ttcttttcag ggcgcgggcg
     1801 tttcgctcga tccacgtgtc ggcttcggca aaatacgcga atttttcctc gtcgcggcga
     1861 aaggcgggcg tgtggttgta gcgcctgccg ttgcgcagaa tcgtcgggtg cttgatatgc
     1921 agcatctcgt gatagacgac gtattcggtg acgaaagcgg gaactttttt atcgtccagc
     1981 gatttgctga tgaccagagt ctcgtgcgtc gaatcgtgat gaccgagccg gtgaaaagtt
     2041 ttttgcgccg accacgtcaa aacgggtttc ggcagccggt tttgaaaata aattcggttc
     2101 aggcggtcga aaatttcttc caaatcgtaa acttcgcctt tcgaagtggt gacgattttt
     2161 cgcccgcgac gtcgccgatt ttcaaaagcc tgcgcctgaa tgctctcggt tctgacaaag
     2221 gcttgataat tttccgaatc cgcccgcggg gcgcgccgcc gcaaaatttt gctgaccaga
     2281 atcgaagcca gcgatttctg gacttccagc ggcgcggctt cgaaaatctg cgcgatgcgg
     2341 acgaagactt tgccc
//
LOCUS       DASWNE010000154         1516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_428740, whole genome shotgun sequence.
ACCESSION   DASWNE010000154 DASWNE010000000
VERSION     DASWNE010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428740"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1304)
                     /locus_tag="VGB68_03190"
     CDS             complement(<1..1304)
                     /locus_tag="VGB68_03190"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEY0048265.1"
                     /translation="MKFTRREILTAFLGLPFALSACGSNSENQAFPDGEIVGANAGLG
                     HILRENRSFEVPAQNWESVKVAVIGGGAAGLAAAWKFQKENFTDFVLLELESAIGGTA
                     RSGKNNFIGYPWGAHYLPVPFKENAELIALLDEMHLTEGRNERGEIVVAEQFLCREPE
                     ERVFYKGRWYEGLYLHAGESAEDKRQFAEFQKQIDFWVNWRDARGKRAFVLPIAECSN
                     DAEATALDKISFAEWLAQKGFDSPRLVWYCDYACRDDYGLKLEQTSAWAGLFYFCSRV
                     RAAGEESQAFITFPEGNGRFVNFLHDKVKEKSRLNTVAVEIIPNEKGVDVVYLDAEKQ
                     IRGLHCRKVVYAAPVFTAKYLIRDFKQNAPDYIGEFEHNAWFVANLFLKDRPKSPFQR
                     DFPLAWDNVIYESNSLGYVNAAHQKGIDHGQTVFTYYFPMCST"
BASE COUNT          380 a          386 c          362 g          388 t
ORIGIN      
        1 gtcgagcaca tcgggaaata atatgtgaaa accgtctgac cgtggtcgat tcctttctga
       61 tgcgccgcgt tgacgtagcc gagagagttg ctttcgtaaa tcacgttgtc ccaggcaagc
      121 ggaaaatctc tctgaaacgg cgatttcggg cggtctttca gaaatagatt cgccacaaac
      181 caggcgttgt gttcaaattc cccgatataa tccggcgcgt tttgtttgaa atcccgaatc
      241 agatattttg ccgtaaaaac gggagcggca tagacgactt tccggcaatg aagcccgcga
      301 atttgttttt ccgcgtcgag ataaacgaca tcgacgcctt tttcattcgg aattatctcg
      361 acggcaaccg tgttcaagcg tgatttttct ttgactttat cgtgcagaaa attgacgaaa
      421 cgcccgttgc cttcgggaaa agtgataaac gcctgcgatt cttcgcccgc cgcgcgcacg
      481 cgcgagcaga aataaaaaag tccagcccag gcggaagttt gttccaattt caaaccgtaa
      541 tcgtcgcggc aggcgtaatc gcaataccag acgagacgcg gtgaatcgaa gcctttttgt
      601 gcgagccatt cggcaaagga aattttatcg agcgcggtcg cttccgcgtc gttcgaacat
      661 tcggcgattg gcaaaacgaa agcgcgtttt ccgcgcgcgt cgcgccagtt gacccagaaa
      721 tcgatttgtt tttgaaattc ggcaaactgg cgtttgtctt ccgcgctttc gccggcgtgc
      781 aggtataagc cttcgtacca gcgaccttta taaaaaactc tctcttccgg ctcgcgacac
      841 agaaattgtt cggcgacgac gatttcgccg cgctcgtttc tgccttcggt cagatgcatc
      901 tcgtcgagca gcgctatcaa ttcggcgttt tccttaaacg gaacgggcag ataatgcgcg
      961 ccccacggat agccgataaa attatttttg ccgctccgcg ccgtgccgcc gatggcgctt
     1021 tccagttcga gcaaaacaaa atccgtaaaa ttttctttct ggaatttcca agccgccgcc
     1081 agtcccgccg cgccgccgcc gataacggcg actttcacgc tttcccaatt ctgcgccgga
     1141 acttcaaacg agcgattttc gcgcaaaata tgacccaagc ctgcgtttgc gccgacgatt
     1201 tcgccgtctg gaaaagcctg attttcagaa ttcgagccgc acgccgagag cgcgaaagga
     1261 agtccgagaa aagccgttaa tatttcgcgt cgcgtgaatt tcattggaaa aaatttacag
     1321 ggataacagg ataaacacgg atgatgattt accgggtaaa gaaatccttc aggctatcag
     1381 ttgtttcaac aaatttattg ttaaaacgtc gatattcaag ttttgcattg ccgaaattga
     1441 ttaacaaacc aactttgatg ccggtcgatt tcaaataatt aattgtttgt gcgtaaaatt
     1501 ctttggtcaa gccatc
//
LOCUS       DASWNE010000155         1938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_569970, whole genome shotgun sequence.
ACCESSION   DASWNE010000155 DASWNE010000000
VERSION     DASWNE010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569970"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..651
                     /locus_tag="VGB68_03195"
     CDS             109..651
                     /locus_tag="VGB68_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016102133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEY0048266.1"
                     /translation="MFFSIGTVALSKASYKPKSVLDFTMKDIDGKDVRLKQYKGNVLL
                     VVNTASKCGYTPQYESLQAVYDKYKAEGFYVLGFPANNFGGQEPGTAAEIKEFCTSKY
                     KVQFPLFAKISVKGEDQHPLYQFLTGKETNPNFAGDISWNFNKFLVDRKGKVVARFDS
                     KAKPDGEAVTQAIEKYLKEK"
     gene            740..1756
                     /gene="lpxD"
                     /locus_tag="VGB68_03200"
     CDS             740..1756
                     /gene="lpxD"
                     /locus_tag="VGB68_03200"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEY0048267.1"
                     /translation="MTVQEIADFIKGNLSGASDVEIKRVAAFESARENEIAFLEKAEP
                     GAATNAACVIVAEDFNGSLACAAIRVKNPKLAFAKIAAVLHAPKKREPEIHQSAIIAE
                     NAEIGNSVFIGAFVCIGENSEIGEGTQIRAGAKIGDNVRIGAGCTIHPNVFIEDNSII
                     GDNVVLHAGAIIGADGFGYVRDGADGYVKFPQIGAVVIEDDVEIGANTCIDRGALGET
                     RIGAGTKIDNLVQIAHNVQIGRRVVIASQTGISGSVTIEDDCIIGGQVGFGDHIHVRK
                     GAVIGSKAGILPGKIVREGVWWGIPIQPLGEYKRQNAMVKGLPRMREEIKELKKQIEE
                     LKSK"
BASE COUNT          551 a          489 c          484 g          414 t
ORIGIN      
        1 gaactgataa ttaaaacccg attcttaact ctcaattatc ggttctcgat tctcagtcat
       61 tttagaggag taaatattat gttcgttaaa attttatgcg gtctgctgat gtttttttca
      121 atcggaacgg ttgctttgag caaagcttcc tataaaccga aatccgttct cgatttcacg
      181 atgaaagaca tcgacggcaa agacgtcagg ctcaaacaat acaaaggcaa cgttctgctg
      241 gtcgtcaaca ccgccagcaa atgcggttac acgccgcaat acgaaagcct gcaggcggtt
      301 tacgataaat ataaagccga aggtttttat gttctcggat tcccggcaaa caatttcggc
      361 gggcaggaac cgggcacggc agccgaaatc aaggaattct gtacgtcgaa atacaaagtc
      421 caatttcctt tgttcgccaa aatctcggtc aagggcgaag accagcatcc gctttatcaa
      481 tttttgaccg gcaaggaaac gaatccgaat ttcgcgggcg atatttcgtg gaacttcaat
      541 aagtttctgg tcgatagaaa aggcaaagtc gtcgcgcgct ttgattccaa agcaaagccc
      601 gacggcgaag ccgtaacgca ggcaatcgag aaatatttga aagaaaaata atttgccatc
      661 gacggaaaac acgaagaaac gctaaatttt tttaatggga atttcttcgt gtttcttcgt
      721 gtttttttcg cagctaaaaa tgacagtcca ggaaattgcc gattttataa aaggaaacct
      781 gagcggcgcg agcgacgttg aaatcaaacg cgtcgccgct tttgaatcgg cgcgggaaaa
      841 cgaaatcgct tttctcgaaa aagccgaacc gggcgcggcg acaaatgccg cgtgcgttat
      901 cgttgcggaa gattttaacg gctcgcttgc ctgcgcggcg attcgagtca aaaatccgaa
      961 gctcgctttc gccaaaatcg ccgccgttct gcacgcgccg aaaaaacgcg agccggaaat
     1021 tcatcaaagc gcaatcatcg ccgaaaacgc cgaaatcgga aacagcgttt ttatcggcgc
     1081 gtttgtctgc atcggtgaaa actcggaaat cggcgaagga acgcaaattc gcgcgggcgc
     1141 aaaaatcggc gataacgtcc gcatcggcgc gggctgcacg attcatccga acgttttcat
     1201 cgaagacaat tcgattatcg gcgataacgt ggttcttcac gcgggcgcga taatcggcgc
     1261 ggacggtttc ggctacgtgc gcgacggcgc ggacggctac gttaaatttc cgcaaatcgg
     1321 cgcggtcgtc atcgaagacg atgtagaaat cggcgcgaat acctgtatcg accgcggcgc
     1381 gctcggcgag acgcgcatcg gcgcgggcac gaagattgat aatctcgtcc aaatcgcgca
     1441 caacgtccaa atcggcagac gcgtcgtgat tgcctcgcaa accggaatct ccggcagcgt
     1501 caccatcgaa gacgactgca tcatcggcgg tcaggtcggt ttcggcgacc acatacacgt
     1561 ccggaaaggc gccgttatcg gctcgaaagc cggaatcctg ccgggcaaaa tcgtccgcga
     1621 aggcgtctgg tggggcatcc cgattcagcc gctcggcgaa tacaaacgcc agaacgcgat
     1681 ggtcaaaggc ttgccccgaa tgcgcgagga aataaaggaa ttgaagaagc aaatcgaaga
     1741 gctcaaatca aaataaaacg gcatgtaaac gccgataaaa acgggtgatt aaaaccaatc
     1801 ccgacgagcc gcccgataaa acaatccagc cgggacattt attttaattc gcccgccgca
     1861 attaaatccg agcattttac cctgaccgaa aatgcgccga acgtctttac aatcgaaatc
     1921 acaaaccgct aaatttcc
//
LOCUS       DASWNE010000156         1702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_110763, whole genome shotgun sequence.
ACCESSION   DASWNE010000156 DASWNE010000000
VERSION     DASWNE010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110763"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="VGB68_03205"
     CDS             complement(<1..460)
                     /locus_tag="VGB68_03205"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEY0048268.1"
                     /translation="MDVLWSPWRYDYIKADDRRLTEIVNGCVFCTILNDSASDEEKFI
                     LHRAEFNFVILNIYPYTSGHLMVVPFAHLADLDRADKKTTDELMDLTKRCQTAIREIY
                     QPNGINLGMNLGKAAGAGVAEHFHMHILPRWFGDVNFMTAIGETRTIPEDL"
     gene            complement(538..813)
                     /locus_tag="VGB68_03210"
     CDS             complement(538..813)
                     /locus_tag="VGB68_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEY0048269.1"
                     /translation="MTKADIVEQVSREAEMTKKDAEQLVEIVFDSIIEALNKGEKVEL
                     RGFGSFRTRQRGARKGRNPKSGAAVDIPAKRVAYFKPGKELKEVIND"
     gene            complement(861..>1702)
                     /locus_tag="VGB68_03215"
     CDS             complement(861..>1702)
                     /locus_tag="VGB68_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEY0048270.1"
                     /translation="VATHRPGDVVRGKISRFAGFGVFVELGDELEGLCHISELSDERV
                     NRAEDVVQLGQELDFKILRIEADTQKIGLSHRAVGKEDEPQVDTKVYTTEAKGGMASL
                     GELANLKFGREPEQPKPEEAKPEPKKEEPKAEEASAETTEASSETETSREGSEENTAE
                     VSSEDKADEASSETSAEATGATAETASEESAVTEASGSESAETPAGESSDAAVGATAE
                     ESGAAAAAGESSETAGETAGTANAETTAATGEETAAQAGETAAAAPEGGDEKNEAEAE
                     KTA"
BASE COUNT          314 a          471 c          444 g          473 t
ORIGIN      
        1 gtaaatcttc gggaatcgtg cgtgtttcgc cgattgccgt cataaaattc acgtcgccga
       61 accagcgcgg caggatgtgc atatggaaat gctcggcgac gcccgcgccg gcggctttgc
      121 cgaggttcat tccgagattg attccgtttg gctggtaaat ttcccggatt gccgtctggc
      181 agcgtttggt taaatccatt aattcgtcgg ttgttttttt atcggcgcgg tctaaatcgg
      241 cgagatgcgc gaaaggaaca accatcagat gaccgctggt gtatggataa atatttaaaa
      301 tgacaaaatt aaactcggcg cggtgaagga taaatttttc ctcatcgctt gccgagtcgt
      361 ttaaaatcgt gcagaaaacg cagccgttga caatttcggt taagcgccgg tcgtcggctt
      421 taatataatc gtagcgccag ggactccaca atacatccac ttttttgagt cgcgggtgga
      481 gagtcgaaag aaaacagaga aatcattctc gactctctaa tcctgactct cgactgatta
      541 atcgttgata acttccttca actctttgcc gggtttaaaa taggcgacgc gttttgccgg
      601 aatatcgacg gcggcgccgg atttcgggtt gcgacctttg cgagcgccgc gctggcgggt
      661 acgaaaactg ccgaagccgc gcagttcgac cttttcgcct ttgtttaatg cctcgataat
      721 gctgtcgaaa acgatttcga ccagctgttc ggcatctttt ttcgtcattt ccgcttcacg
      781 ggaaacttgt tcaacaatat ctgcctttgt catgcaatta acctcacggg gaatctaaac
      841 tttaattaca agaactcaag ttacgccgtt ttctcggctt cggcttcgtt tttctcatct
      901 ccgccttccg gtgcggctgc ggcagtttcc ccagcctgag cggcggtttc ttcgcctgtc
      961 gcggcggttg tttccgcatt cgccgttccc gcggtttcgc cggcggtttc cgaagattcg
     1021 cctgcggcag ccgctgcgcc ggattcttcc gccgtcgcgc cgacagcggc gtcagacgat
     1081 tctcccgccg gagtttcggc gctttcgctg cctgaggctt ccgtcacggc agattcttcg
     1141 cttgccgttt cagcggttgc gccggttgct tcagccgaag tttccgaaga agcttcatcg
     1201 gctttgtctt ccgatgaaac ttccgcagtg ttttcttccg acccttcgcg ggacgtttcc
     1261 gtttcagatg acgcttcagt cgtttcggca gacgcttctt cggctttcgg ctcttctttt
     1321 ttcggttcgg gtttggcttc ttcaggtttc ggctgttcgg gttccctgcc gaatttcaga
     1381 tttgccagct cgcccagcga tgccattccg cctttggctt cggtcgtgta gactttcgta
     1441 tcgacctgcg gctcgtcttc cttgccgacg gcgcgatgcg agagaccgat tttctgcgta
     1501 tcggcttcga tgcgaaggat tttgaaatcc agctcctgac cgagctgcac gacgtcttcg
     1561 gcgcgattga ctctttcatc ggacaattcc gagatgtgac acaagccttc gagctcgtcg
     1621 ccgagttcga caaaaacgcc gaatccggca aagcgcgaaa tcttgccgcg cacgacatcg
     1681 cccgggcgat gcgtcgccac ga
//
LOCUS       DASWNE010000157         1798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_675372, whole genome shotgun sequence.
ACCESSION   DASWNE010000157 DASWNE010000000
VERSION     DASWNE010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675372"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VGB68_03220"
     CDS             complement(<1..186)
                     /locus_tag="VGB68_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048271.1"
                     /translation="MKEQLVQIIVERLTRDEEKLRADFSADKGVRTKFAAIDNLFPED
                     IARKIYEAYPPVEEMRLL"
     gene            368..1633
                     /locus_tag="VGB68_03225"
     CDS             368..1633
                     /locus_tag="VGB68_03225"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEY0048272.1"
                     /translation="MDRRYFLKSSGIALAGFGLMSAAPNFLHQFAEAQTLVNAKGRRK
                     ILITIFQRGAVDGLNMVVPYGDDNYYELRRSIAIQSPNQADGAINLDGYFGLHPSLKP
                     LEAFWKNKQLAVIHSVGSPNNTRSHFDAQDYMESGTPGVKSTRDGWLNRVLQTNKSDK
                     DSPFRAVSMTTQIPRSLIGRAPTVAMTNLADFSIKAGAYTQSVAGGFEGIWQENSKDG
                     LGETGKETFEAVNFLKQANPAQFKPDNGAIYPNTPFGRSLRQIAQLIKADVGLEIAFT
                     DTPGLNWDTHANQGGARGQLANLLRDFGAGLAALAADLGQRMDDVVILTMSEFGRTAR
                     QNGTNGTDHGHGNAMLVLGNSVKGGKVYGDWKGLKPDQLNEGRDLAVTTDFRDVFAEA
                     SMKHLGNKNLDKIFPGYQANAKKFRGFLG"
BASE COUNT          459 a          476 c          465 g          398 t
ORIGIN      
        1 taaaagccgc atttcctcca cgggcggata cgcttcgtaa attttccgcg cgatgtcttc
       61 gggaaaaaga ttgtcaatcg ccgcgaactt ggttctgacg cctttgtcgg cgctgaaatc
      121 cgcacgcagt ttttcctcgt cacgcgttaa tcgctcgaca ataatttgga caagttgttc
      181 tttcataaat agtcggttgt cagtagttcg ttgtcagttg cttgtttgat aataatttac
      241 aacggacttg cgacaactga caacggacga aaaataaaag tgaccaaaca aaattttagc
      301 gcgtctaaat aaacagcgaa acggatttct ttccgaaaat cccggacact ttttaagagc
      361 tgaaattatg gatagaagat attttttaaa atcgagcgga atcgctctcg ccggcttcgg
      421 cttgatgtcg gcggcgccga attttctgca ccagtttgcc gaggcgcaaa cgcttgtcaa
      481 tgcaaagggc agacggaaaa ttctgattac gattttccag cgcggcgcgg tcgacgggct
      541 gaatatggtc gtgccttacg gcgacgataa ttattacgag ctgcgccgca gcatcgcgat
      601 tcaaagcccg aatcaggcgg acggcgcgat aaatctggac ggctatttcg gtcttcaccc
      661 gtcgctcaaa ccgctcgaag ctttctggaa aaacaaacag ctcgccgtca tacattcggt
      721 cggctcgccc aacaacacgc gctcgcattt cgacgcgcag gattatatgg aatccggcac
      781 gcccggcgtc aaatccacgc gcgacggctg gctcaaccgc gttttgcaga cgaacaaaag
      841 cgacaaggat tcgccgtttc gcgccgtttc gatgacgacg cagattccgc gctcgctgat
      901 aggtcgcgcg ccgaccgtcg cgatgactaa tctggcggat ttttccatca aagccggcgc
      961 ttacacgcaa tcggtcgcgg gcggttttga aggcatctgg caggaaaact ccaaagacgg
     1021 tctgggcgaa accggaaagg aaacttttga agccgttaat ttcctgaaac aggcaaatcc
     1081 ggcgcagttc aaacccgata acggggcaat ttatccgaac acgccgttcg gtcgctcgct
     1141 ccgccaaatc gcgcaattga ttaaagccga tgtcggcttg gaaatcgcct ttaccgatac
     1201 gcccggactt aactgggaca cgcacgccaa ccagggcggc gcgcgcggtc agctcgccaa
     1261 cctgctccgc gatttcggcg cggggctggc agcgctcgcc gccgatttgg gtcagcgaat
     1321 ggacgacgtc gtgattttaa cgatgtccga attcggcaga actgcccgcc aaaacggcac
     1381 caacggcacc gaccacgggc acggcaacgc gatgctcgtt ctcggaaact ccgtcaaggg
     1441 cggcaaagtt tacggcgact ggaaaggctt aaagcccgac cagctcaacg aaggacgcga
     1501 cctcgccgtg acgacggatt tccgcgacgt tttcgccgaa gcttccatga agcatctcgg
     1561 caacaaaaat ctggataaaa tctttccggg ctatcaggca aacgcgaaaa aattcagagg
     1621 ttttctcggc taatctccaa aaggtaaacg agaaattaaa aagccgtcgc ttgaaatttc
     1681 aagcgacggc tttttcggtt agttaatatc tttcttaatt ccaaaaaaaa tgcgcggcat
     1741 ttgcgccgcg ccggtaggta tagcttcagg aaggaaaaaa ggtcggggtt agtttaat
//
LOCUS       DASWNE010000158        11544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_464242, whole genome shotgun sequence.
ACCESSION   DASWNE010000158 DASWNE010000000
VERSION     DASWNE010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11544
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464242"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2009
                     /locus_tag="VGB68_03230"
     CDS             <1..2009
                     /locus_tag="VGB68_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048273.1"
                     /translation="WANSFVEEANLTHHIFRLRKVLGASEEKKFIETISKRGYRFVAD
                     VREAQAADFSLPIPGSPNRAAEPAAQNKNIRIFSAIVLIVLFATAAFVWYKSAASVKE
                     NQPPNAKLKTDIAMTISRLTNSGKVAAATISPDGKFAAYAQNHTSGEGMLYIRQIETN
                     TERKLLEPAGRNFGSIAFSPDGTFIYYISYEPGDPQGALYRIPVIGGQAVKVLSDVRF
                     MFSLSPDGKRAAFYRFDEERKQKSIVHAALDGSGDEKTIFTYQAEQTAVSSVPAFSPD
                     NRLLSFSTAEIAPGVDYNAPQFSLFAVDLQSGETKRLTEEKWSEIGKTIWMPDGGGLV
                     FTGRRPRIGNQIYFLSYPSGEVRRITSELNSYGNYGMGVSGDGTTLVADLWESAAQLW
                     AIDSSGTTARAEQLTNGSSDGARGLTSFSDGQIVYTTRTGDDNDLWILHSKDGRREGS
                     PLTTDAFFEGEVCAAPDDSFLIFASDRAGGSHLFRIDRDGSNLKQITFGESFDDAPDC
                     SPDGKFVVFSSNSSIRKVSAEGGEPVRLTDFECVAPSISPDGKFFACIQPTGIQIKNS
                     TLAVVPIEGGAPVKTFEVIPFGFYYRAPRWTPDGSALVFKKTDKQTGNLWRQNLSGGE
                     PKQISDFKSDVIFNHAFTRDGKRLLVSRGKFAANTVMLKNFRPD"
     gene            complement(2006..3043)
                     /locus_tag="VGB68_03235"
     CDS             complement(2006..3043)
                     /locus_tag="VGB68_03235"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY0048274.1"
                     /translation="MQGQAIITKTNFSSRRSIILLAAAFAAVYIFWGSTYLAIKYAIE
                     TMPPFLMAGARFVFAGSILYVWARFSKDYEKPGAAHWRTSFIVGTLLLLGGNGGVVFA
                     EHYISSSLAALLVATEPFWIVLLGWLWLKGARPNLKVALGLVVGFLGVWLLISGQNTS
                     GAGAAAAGGSMQIISTIVIIAAAFSWAVGSIYGLRSPVPKSSLLTAGMQMLSGGAVLL
                     LVSLISGEWSKFSVAEVSANSWFGLVYLIIFGSLVGFTAYSWLLKNAPPAMVATYAYV
                     NPVIAVFLGWLIAGESFTGQMLVGASIIVGSVVLITSHNKEKEEEALPENEIHRSQIS
                     SENSRALSSSA"
     gene            complement(3073..3570)
                     /locus_tag="VGB68_03240"
     CDS             complement(3073..3570)
                     /locus_tag="VGB68_03240"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY0048275.1"
                     /translation="MINDIDKQILNIVQQDARISNAEIARQVGLAPSAVLERIRKLEE
                     RGVIHGYETKIDGAQIGFGLTAFVFVRTSVCGELDETLAQIPEVLEVHDVAGEDCYLL
                     KVRAENTEELGNLLREKLKPIPEIISTRTTVVLKTIKETTALPLSKLNEDSAVEPKRR
                     GKKDK"
     gene            3742..4401
                     /locus_tag="VGB68_03245"
     CDS             3742..4401
                     /locus_tag="VGB68_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048276.1"
                     /translation="MRTIIFSLLFFLFLNCVSAQTIAAQKISLRGNKTGGIVLTKGKA
                     RATINLRADVAGCPYLADGEYKRDLDARECTAPPAGFKLIDATVKNNQIYLVVLSEAM
                     GNCNVCGQCGASEAFALVWLRLDANLRLIKKKSVPLSLCRENISMVSPQIINEAADNE
                     TLNLSFKAGVLRIELEKEIYGENNTTGYEFSRLEYNRKTPEKGFVIKTEKRAESAQRD
                     Q"
     gene            4410..5990
                     /locus_tag="VGB68_03250"
     CDS             4410..5990
                     /locus_tag="VGB68_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0048277.1"
                     /translation="MSAVLSFPTKQEQIVSCNPATGEEIGRVPAGSAEDVKIAVEKSR
                     AAFLKWRETSFKERARVVMKAREVILAEMEEIARLISDESGKPVAEALSMEIAPVLDL
                     MQYFARHAPKILKPEKINIGLYAALGRYSKIVYQPLGVVGIIPAWNYPFSIPLGETAM
                     ALLAGNTVILKPSELTPFTGLKIGEIFQKAGLPAGIVQVLTGDGRTGAALVDAGVNKI
                     MFTGSVGTGRKIAESAAKHLTSVVLELGGKDPMIVFKDANLNLAAEAAIWGAFCNSGQ
                     SCSSVERLYVEESIREEFTNLVVEKTKRLRQNYGSDESTDIAAMSSERQLKLVEDHVR
                     SFTEEGAKILTGGKRNAKFEGSFFEPTVIGNADNSMRAMREETFGPTLPIAVFKTEAE
                     AIRLANDSEFGLTASVWTKDLTKGKRVAEKIEAGTVCVNEVLYTHGIGQTPWGGFKNS
                     GYGRTHGRQGLLELVQPQHIHVNRFAILPNAWWLPYSPEAVKTFRGFARYFATGSLFQ
                     TMKLAPQLLRRIRELRKK"
     gene            6305..6577
                     /locus_tag="VGB68_03255"
     CDS             6305..6577
                     /locus_tag="VGB68_03255"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEY0048278.1"
                     /translation="MPKYVIERELPGAGKLSPQELQAISQKSCGVLNNLGTSIQWLQS
                     YVTDDKIYCVYIAPNEEIVREHAQQGGFPANSIAEVRMIIDPTTAE"
     gene            complement(6660..7604)
                     /locus_tag="VGB68_03260"
     CDS             complement(6660..7604)
                     /locus_tag="VGB68_03260"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0048279.1"
                     /translation="MRFKFYIFVLVVLAFASSDFALAQPNKPAPAATARSITVVTEPN
                     AIVWIDDIRRGTTDASGKLFIKPVPSGIRKLRVRAEGFKEAAQPLTAAQKGEVKVALV
                     KTADEAELAFQQAETAASADREKSIELYRRAVKLRPRFAEAQLGLARVLLDNNDNEEA
                     LKAVREARKTKPIYPEASAVEGRIYRSEGDDEKAVVAFKKAIREGKGFQPEAHTGLGL
                     LYKERAEGFGSEGDLEQEKVNYDLAAAAFKTALQQLGGAPDAEIIYQMLGVTYEKQQK
                     YAEAIRIYEEFLRVFPDSTEASAVRSFIVQAQKQMKEQ"
     gene            8062..9102
                     /locus_tag="VGB68_03265"
     CDS             8062..9102
                     /locus_tag="VGB68_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1508 domain-containing protein"
                     /protein_id="HEY0048280.1"
                     /translation="MLVREVMSDNPACCTPDSSLQEVAQMMIENDCGCIPVVDNQSNR
                     KPVGTITDRDITIRTVAAGQNPVTMKASDIMSIDIATVKPDSSLEECFDAMEARNIRR
                     ILVVDEKGGCCGIVAQADVAQYSKNPARTTEFLRDISESSQSHNMTMSKDLESNKSSV
                     NTRSFLPLLFGVGSGAALAYFLGGRKARHQETGNSNTKQYLDAESEVNKRQHNLQNRF
                     EELKKETRAPITGSVESNPGRFEIKTSANGKFHFNLKTGNGETVLSSELYNSRSAVEK
                     GIESVKQNADDANRFERKLNKNDEPYFVLKAGNGEVIGTSEVFSSETALENAISAVMK
                     SAPTASISNTAS"
     gene            complement(9191..9370)
                     /locus_tag="VGB68_03270"
     CDS             complement(9191..9370)
                     /locus_tag="VGB68_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048281.1"
                     /translation="MDWKEKINDLALKGLISYGTENELQDFISTEIIEKLIADIPDGD
                     NSLESIKQQLRDKWL"
     gene            9443..9772
                     /locus_tag="VGB68_03275"
     CDS             9443..9772
                     /locus_tag="VGB68_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048282.1"
                     /translation="MNFGENKSSAIILRKSALHKNKENQEKFRWLIVNSQLLILNYFT
                     KDTAHQFRIYNFPFLYFCAKPRKSFETLSLKLYSTDEVELSFVTHQAFKCVTGAFGYF
                     FYQGVKG"
     gene            9769..10272
                     /locus_tag="VGB68_03280"
     CDS             9769..10272
                     /locus_tag="VGB68_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048283.1"
                     /translation="MKRLLALTLSIVSIGFIASSAEAKTDSSLAGTATANAVRQITIR
                     IGSGNRRYNRRYNRGYNRAYNRGYYRTYSRAYYPSYNRRYTRSYSRSYYPTYSRSYSR
                     TYTRVYYPTYNTGYYRTYNRGYSRYNGGYNSSLRRAYRRGFVRGYTRGANRSYQRRYA
                     TRRYVIY"
     gene            10529..10605
                     /locus_tag="VGB68_03285"
     tRNA            10529..10605
                     /locus_tag="VGB68_03285"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10563..10565,aa:Met,seq:cat)
     gene            10752..>11544
                     /locus_tag="VGB68_03290"
     CDS             10752..>11544
                     /locus_tag="VGB68_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048284.1"
                     /translation="MKICPKCHSSYSDETLNFCLTDGVPLVAEEAYGGRLSSQHSWQE
                     AETLHDSNFVFPSTGEHTTSPNSGSPTFASSASHNTSINVIPPKKSSNRVLLSIVGAL
                     LALGTAGGAFWYFAGRDTNSSNSSGSIPTATVEAKKTSGALNAEQENQIKKEITAMME
                     TWRSTIEKHDIDTHIKSYVATLDNYYKESSVHREHVRADRLRAFERYPTINLQIDNIK
                     ITPHSNVSATVVFDKTWTFKRPDRTSTGSVQQEMELVKQDGRWLIN"
BASE COUNT         3003 a         2939 c         2895 g         2707 t
ORIGIN      
        1 tctgggcaaa cagctttgtc gaggaagcga acctgacgca tcatattttt cgcctcagaa
       61 aagttctggg cgcaagcgaa gaaaagaaat ttatcgaaac catttcaaaa cgcggctatc
      121 gttttgtcgc cgacgtgcgg gaagcgcaag cggcagattt cagtcttccg attccgggct
      181 cgccgaaccg cgctgccgag cctgcggcgc aaaacaaaaa tatacggatt ttctccgcca
      241 ttgttttaat cgttcttttc gcgacggctg cttttgtctg gtataaatcg gcggcaagcg
      301 taaaggaaaa ccagccgccg aatgcaaagc ttaaaaccga cattgcgatg acgatttcgc
      361 gcctgacaaa cagcggcaaa gtcgccgccg caactatctc gccggacgga aaattcgcgg
      421 catacgcgca gaatcacacg agcggcgagg gtatgctcta tatccggcag atcgaaacga
      481 acacggaaag aaagctgctt gagcccgccg gcagaaattt cggcagcatc gctttttcgc
      541 ccgacggcac atttatttat tacatctctt atgagccggg cgacccgcag ggcgctctgt
      601 atcgtattcc ggttatcggc ggtcaggcgg tcaaagttct gtctgacgtc agatttatgt
      661 tttcgctttc gccggacggc aaacgcgccg cgttttaccg cttcgacgaa gagcgaaaac
      721 aaaaaagcat cgttcacgcc gcgctggacg gaagcggcga cgaaaaaaca atcttcacgt
      781 atcaagccga gcagacggct gtttcgagcg ttccggcatt ttcgcccgac aatcgcctgc
      841 tttcgttcag cacggctgag attgcgcccg gcgtggatta caacgcgccg cagttttcgc
      901 ttttcgcagt cgatttgcag agcggcgaga caaagcgact gaccgaagaa aaatggtcgg
      961 aaatcggcaa aacaatctgg atgccggacg gcggcgggct ggttttcacc ggcagacgtc
     1021 cgcgcatcgg caatcaaatt tattttcttt cttatccttc gggcgaagtc cggcgcatca
     1081 ccagcgaatt aaactcctac ggcaattacg gaatgggcgt cagcggtgac ggaacgacgc
     1141 tggttgccga tttatgggaa agcgcggcgc agctctgggc gattgattcg agcggcacga
     1201 cggcgcgagc cgagcaatta accaacggaa gcagcgacgg cgcgcggggc ttaacgagct
     1261 tttccgacgg tcaaatcgtc tacacgacgc gcaccggcga cgataacgat ttatggattc
     1321 tgcacagtaa agacggaagg cgtgaaggca gtccgctgac gacggacgct ttttttgaag
     1381 gcgaagtttg tgctgcgccg gacgacagtt ttttgatttt cgcttccgac cgcgccggcg
     1441 gctcgcatct ttttcgcata gaccgcgacg gctcgaatct taaacaaatc actttcggcg
     1501 aaagcttcga tgacgcgccc gattgctcgc ctgacggcaa atttgtcgtt ttcagctcta
     1561 attcgtcaat ccggaaagtc tccgcggaag gcggcgagcc ggttcgattg accgattttg
     1621 aatgtgtcgc gccgagcatt tcgcccgacg ggaaattttt cgcctgcatt cagccgaccg
     1681 gaattcaaat caagaattcg acgctcgcag tcgttcctat agaaggcggc gcgccggtaa
     1741 aaaccttcga ggttatccca ttcggttttt attatcgcgc gccgcgctgg actccggacg
     1801 gcagcgccct cgtttttaag aaaaccgata aacaaaccgg caatctctgg cggcagaatc
     1861 tttcgggcgg cgaaccgaaa caaatttcag actttaaatc cgacgtgatt ttcaatcatg
     1921 ctttcacgcg ggacggcaag cgattgctcg tctcgcgcgg aaagtttgcc gccaacaccg
     1981 tgatgttgaa gaattttcga cccgattaag ccgaactcga taaagcccgg ctattttccg
     2041 acgaaatctg cgaccggtga atttcatttt ccggaagcgc ttcttcttct ttctctttat
     2101 tgtgcgaagt tatcagcacg accgagccga caatgatgct cgcgccgacg agcatctgcc
     2161 cggtaaaaga ttcgccggcg atgagccagc cgagaaaaac ggcgatgacc ggattgacat
     2221 aggcgtaggt ggcaaccatc gccggcggcg cgttttttaa aagccagcta tacgcggtaa
     2281 agccgacgag cgagccgaaa atgattaaat agaccagtcc gaaccacgaa tttgccgaaa
     2341 cttcggcgac gctgaatttc gaccattcgc cggaaatcaa actcaccaga agcaaaaccg
     2401 cgccgccgga aagcatctgc attccagccg tcagcagcga cgatttcgga accggcgagc
     2461 gcagaccgta aatcgagccg accgcccacg aaaaagccgc cgcgataatg acaatcgtgc
     2521 tgataatctg catcgagccg cctgccgcag ccgctcccgc gccgctcgta ttttgaccgc
     2581 tgattaaaag ccagacgccg agaaagccga ccaccagacc gagcgcgact ttaaggttcg
     2641 gacgcgcgcc tttcagccag agccacccga gcagcacaat ccagaaaggc tcggtcgcca
     2701 ccagcagagc ggcaaggctc gaagaaatat aatgttcggc gaacacgacg ccgccgttgc
     2761 cgcccaaaag cagcagcgtt ccgacgatga aactcgttcg ccaatgagcc gcgcccggct
     2821 tttcgtaatc tttggaaaag cgcgcccaga cgtaaagaat cgaacccgca aaaacgaaac
     2881 gcgcgcccgc catcagaaac ggcggcatgg tttcaatcgc gtatttgatt gccagataag
     2941 tcgagcccca gaaaatataa accgccgcaa acgccgccgc caataaaatt atgctgcggc
     3001 ggctcgaaaa atttgttttt gttataattg cctgtccctg catgtctgaa aaacccctta
     3061 acctttaaga tttcatttgt cttttttgcc gcgtcttttc ggctcgacgg ctgaatcttc
     3121 atttaattta ctgagcggca aagcagtcgt ttccttaatg gttttaagca ccacggtcgt
     3181 ccgcgtcgat atgatttcgg gtatcggctt cagcttctcg cgcaacagat ttccgagctc
     3241 ttccgtgttt tcggcgcgaa ctttcagcag ataacaatcc tcgcccgcga cgtcgtgaac
     3301 ctctaaaact tcgggaatct gcgccagcgt ctcgtcaagc tcgccgcaga cgctcgtccg
     3361 cacgaaaaca aaagccgtca agccgaaacc gatttgcgcg ccgtcgattt tcgtctcgta
     3421 gccgtgaatc acgccgcgtt cttccagctt gcgaatccgc tcgagcacgg cggacggcgc
     3481 aagcccgacc tgccgcgcga tttccgcatt ggaaatccgc gcatcttgct gaacgatgtt
     3541 cagtatttgt ttgtcgatat cattaatcat tcttaatttt caaccatttt cagaataaca
     3601 ttctgaaaat tgactgtcaa tatttcattt aaccgggcaa atattttttg gcggaaaccg
     3661 tttattgccc gcaatcggat agagtattcg tgccctgaca ttcggaaatg tgttatcttt
     3721 ccaaataatt tttaaggatt tatgcgaacg ataattttca gcttgttatt tttcctgttt
     3781 ctaaattgcg tttcggcgca aacgattgcg gcgcaaaaaa tcagcctgcg cggcaacaaa
     3841 acgggcggga ttgtcttgac gaaaggaaaa gcgcgggcga cgataaacct gcgcgcggac
     3901 gtggcgggct gcccgtatct cgccgacgga gaatacaagc gcgatctgga cgcgcgcgaa
     3961 tgcaccgcgc cgcccgccgg ttttaaattg attgacgcga ccgtcaaaaa caatcagatt
     4021 tatctggtcg ttttgtccga agcgatgggc aactgcaacg tctgcgggca atgcggcgcg
     4081 agcgaggcgt ttgcgctcgt ctggctacgg ctcgacgcaa atctgcgttt gattaaaaag
     4141 aaatccgtgc cgctctcgct ttgccgggaa aacatttcaa tggtcagccc gcaaataata
     4201 aacgaagccg ccgataacga aactctgaat ttatcgttta aagcgggcgt tttgaggata
     4261 gaactcgaaa aggaaattta cggcgaaaac aatacgacgg gctatgaatt ttcgcgtctg
     4321 gaatataacc ggaaaacacc ggaaaagggt tttgtcatca aaaccgaaaa acgcgccgaa
     4381 tcggcacaac gcgaccaata ggagaattta tgagcgcagt tctttcattt ccgaccaaac
     4441 aggagcaaat cgtttcctgc aatccggcga ccggcgaaga aatcggcaga gttccggctg
     4501 gctcagccga agacgtcaaa attgcggtcg aaaaatcgcg cgcggctttt ctgaaatggc
     4561 gcgaaacttc gtttaaggaa cgcgcccgtg tagtgatgaa agcccgcgaa gtgattctgg
     4621 cggaaatgga agaaatcgcg cgcctgattt ccgacgaatc gggcaaaccg gtcgccgaag
     4681 cgctctcgat ggaaatcgcg cccgtactgg atttaatgca gtattttgcg cgccacgcgc
     4741 cgaaaattct caagccggaa aaaattaata tcggtcttta cgccgcgctc gggcgctatt
     4801 cgaaaatcgt ctatcagccg ctcggcgtgg tcggaatcat tccggcgtgg aattacccgt
     4861 tttcaattcc gctgggcgaa acggcgatgg ctctgctggc gggaaatacc gtaattttga
     4921 aaccgagcga gctgacgcct tttaccggtt taaaaatcgg cgaaattttt cagaaagccg
     4981 gtctgcccgc cggtatcgtg caggttctaa cgggcgacgg cagaaccggc gcggctttag
     5041 tcgacgcggg cgtcaataaa attatgttta ccggaagcgt cgggacgggc aggaaaatcg
     5101 ccgaatcggc ggcgaaacat ttgacctcgg tcgtgctcga actcggcggc aaagacccga
     5161 tgattgtctt caaagacgcc aatttgaatc tcgcggcgga agcggcaatc tggggcgcgt
     5221 tctgcaattc cggtcaatcc tgctcgtcgg tcgaaaggct ttacgtcgag gaatcaatca
     5281 gggaagaatt tacgaatctg gtcgtcgaaa agacaaagag gctgcggcag aattacggca
     5341 gcgacgaatc gaccgacatc gccgcgatgt cgagcgaaag acaattgaaa ctggtcgaag
     5401 accacgtccg aagctttacg gaagaaggcg cgaaaatcct caccggcgga aagcgcaacg
     5461 caaaatttga aggctcattt ttcgagccga ccgtcatcgg aaacgccgac aattcgatgc
     5521 gcgcgatgcg cgaggaaact ttcggtccga cgcttccgat tgccgttttc aaaaccgaag
     5581 cggaagcgat tagactggcg aacgattcgg aattcggctt gacggcgagc gtctggacaa
     5641 aggatttgac gaaaggaaag cgagttgccg aaaaaatcga agccggaacg gtttgcgtca
     5701 acgaagtttt atacacgcac ggcatcgggc aaactccgtg gggcggcttt aaaaattccg
     5761 gttacggcag aacgcacggg cgtcagggct tgctggaact cgtccagccg cagcatattc
     5821 acgtcaaccg cttcgccatt ttgccaaacg cctggtggct tccctattcg cccgaagccg
     5881 tcaaaacttt tcgcggtttc gccagatatt tcgcgaccgg ctctctgttt cagacgatga
     5941 agctcgcgcc gcagctttta agacgcatca gggaattaag aaaaaagtaa aaagtaaaaa
     6001 gtaaaaagta aacttccggc ggacgatttc gcgttggaaa cataaaattt tcgatttcgc
     6061 gttttccatt gtctggcgca cgaaattcac gctttttggc tcggaaggaa acttttgaga
     6121 aatcgaaaac tttttagcgc cttatttgag tttctattaa tcacattttg ttttcttgcc
     6181 gccggcgcgc aggcaaggac aatcaaagcg aataaaatcg aaccgcaatg gattttctga
     6241 aacggacagg ataaaatccc tgctgtaatt tccagataaa acactaaaat cgaaaggaaa
     6301 aattatgccg aaatatgtta tcgaacgcga attgccgggc gccggcaagc tttcgccgca
     6361 ggagcttcag gctatttcgc agaagtcctg cggcgttcta aacaacctgg ggacgtcaat
     6421 tcaatggctg caaagctacg tcaccgacga taagatttac tgcgtttaca tcgcgccgaa
     6481 tgaagagata gtgcgcgaac acgctcagca aggcgggttt ccagccaaca gcatcgccga
     6541 agtgcgaatg ataatcgacc cgacgaccgc cgaataaagt cttacgaaac cgagaggcgg
     6601 gaaccggtaa tgatgaaaag attttcggct ctcgcttccc ggttttgcct ttttactttt
     6661 tactgttcct tcatctgttt ctgcgcctgc acgataaacg agcggacggc gctggcttcc
     6721 gtgctgtcgg gaaaaacgcg cagaaattct tcgtaaatcc tgatggcttc ggcgtatttc
     6781 tgctgttttt cgtatgtgac gccgagcatc tggtaaataa tctcggcgtc gggcgcgccg
     6841 ccgagctgct gcagagcggt tttaaaggcg gcggcggcga ggtcgtagtt gactttttcc
     6901 tgttccaaat cgccttccga accgaagcct tcggctcttt ccttgtaaag caagcctaat
     6961 cccgtgtgcg cttccggttg aaagcctttg ccttcgcgga tagctttttt gaaggcgacg
     7021 acggcttttt cgtcatcgcc ctcggagcga taaattctgc cttcgacggc tgaagcttcg
     7081 gggtaaatcg gcttggtttt tctggcttcc cgaactgcct ttaacgcttc ttcattatcg
     7141 ttgttatcca aaagcacgcg cgccaagccg agctgcgcct cggcaaatcg cgggcggagc
     7201 ttaacggcac ggcggtaaag ctcgatggat ttttcgcggt cggcgctcgc cgcggtttcg
     7261 gcttgctgaa aggcaagctc ggcttcgtcg gcggttttga cgagcgcgac ttttacttcg
     7321 cctttctgcg cggcggtcaa aggctgcgcg gcttctttaa agccttcggc gcgaacgcga
     7381 agttttctga tgccgctcgg aacgggtttg ataaaaagct tcccgctcgc gtcggtcgtg
     7441 ccgcgccgga tgtcgtcaat ccagacaatc gcgttcggct cggtgacgac ggtaatcgaa
     7501 cgcgccgtcg ctgcgggtgc gggcttgttt ggctgcgcca gagcaaaatc gctcgaagcg
     7561 aacgctaaaa ctactaaaac aaaaatgtaa aatttgaaac gcataagctt aaccctcttc
     7621 gtgtttatga ttacaaaact cgaaacgagc gttgcaaaga caattacgaa attacggagc
     7681 ggacgctcaa aaactttcgc cgcaagccgg tcgaaaacaa tcgtcgaaaa caaatcgaag
     7741 ataactccga ctgcggcgcg ggcggcaaag atgagcgaaa tctgcatcgt aaccttcgtt
     7801 tttacggctt aaatatcttt caataataca ttttccggct ggatttccca aaaaccgatt
     7861 ttttatgaga aagcgaaata ctccgccgaa agaaacaatt tttcgggatt atcgagagat
     7921 agccgtcacc gtttggaaag tgtcgagcga caatttccgg tttctttata tcatttgggg
     7981 aataataatt gcctctgttt ataaattcta aacaagcgta ttaattcgct tcacatttta
     8041 gatagttcaa ggaggaagat aatgctggta agagaagtta tgtcggacaa tcccgcttgc
     8101 tgcacgccgg attcgagttt gcaggaagtc gcgcagatga tgattgaaaa cgattgcgga
     8161 tgtattccgg tggtcgataa tcaatcgaac agaaaaccgg tcggcacgat taccgaccgc
     8221 gatattacta tccggacggt cgccgccggt caaaatccgg tgacgatgaa ggcatcggat
     8281 attatgtcca tcgatattgc taccgttaag cccgattcga gtctcgaaga atgtttcgac
     8341 gcgatggaag caagaaacat cagacgtatc ctggtggtcg atgaaaaggg cggctgctgc
     8401 ggcatcgtcg cgcaggctga cgtcgcgcaa tacagcaaga acccggcgcg caccacggaa
     8461 tttttaagag acatctcgga atcgtcgcaa tcgcataata tgacgatgtc gaaagatttg
     8521 gaaagcaata aatcttcggt caacacccgt tcgtttctgc cgcttttgtt cggcgtcggt
     8581 tccggcgcgg ctttagctta ttttttaggc gggcgcaaag cgcgacacca ggaaacgggc
     8641 aattcaaaca ccaaacaata tctggacgcc gagagcgaag taaataaaag gcagcataat
     8701 ttacaaaatc gcttcgagga actgaaaaag gaaacgcgcg cgccgataac cggaagcgtc
     8761 gaatcaaatc cgggaagatt tgaaataaaa acgtccgcca acggaaagtt tcatttcaat
     8821 ctcaaaaccg gaaacggaga aaccgtttta tccagcgagc tgtataattc cagaagcgcg
     8881 gtcgagaaag gcatcgaatc ggtcaagcaa aatgcggacg acgccaatcg cttcgagcgg
     8941 aaactgaaca aaaacgacga gccgtatttt gtcctgaaag caggcaacgg agaagtaatc
     9001 ggcacgagcg aagttttttc atccgaaacg gcgctggaaa acgccatttc agccgtaatg
     9061 aaaagcgcgc cgacggcgtc catctcaaat acggcgagct aaaacacagc agccagcccg
     9121 cagaaataaa gcccgttgtc attgcagcgg gctttatttc tttatccgag cggcttacag
     9181 ccgaggctgg tcatagccat ttatctctca actgctgttt aatcgactcc aggctgttgt
     9241 ccccgtccgg tatgtccgct atcagctttt cgatgatttc agtagagatg aagtcctgaa
     9301 gctcattttc cgtgccgtag ctgattaatc cttttaaagc gagatcgttt attttctctt
     9361 tccaatccat atatttataa tgacatatta gataaattta agaataaaac cgtctcgacc
     9421 gctcgcaatc aattttgcgg agatgaattt cggcgaaaac aaatcatctg cgattatctt
     9481 acgcaaatct gctttgcaca aaaataaaga aaatcaagaa aaattccgat ggctaattgt
     9541 aaattctcaa ttattaattc tcaattactt cacaaaagat accgcacatc aatttagaat
     9601 ttacaatttt ccctttcttt atttttgtgc aaagccacgc aaatcatttg aaactctttc
     9661 cttaaaattg tattctactg atgaagtcga actctccttt gtgacacatc aagcttttaa
     9721 atgtgtgaca ggcgcgttcg gctatttttt ttatcaagga gttaagggat gaaaagacta
     9781 ttggcactta ctttgtcaat cgtatcaatc ggttttatcg cttcctcagc cgaagctaaa
     9841 acggatagtt ctctggcagg cacggcaacg gcaaatgccg tcagacaaat tacaatcaga
     9901 ataggctcgg gcaacaggcg ctataaccgg cgttataatc gcggctataa ccgagcttat
     9961 aatcgcggat attaccgcac ttacagtcgc gcgtattatc cttcttataa tcgccgctat
    10021 acgcgaagct acagtcggtc atattacccg acttatagtc gcagctattc gcgaacttac
    10081 acccgcgttt actacccgac ctacaacaca ggttattacc gaacctataa tcgcggttac
    10141 agccgctata atggcggcta taactcgtct ctgcggcggg cttacaggcg cggttttgtc
    10201 cgcggctaca cgagaggagc aaatcgcagt taccaaaggc gatatgcgac gagaagatac
    10261 gtgatttatt aatccgtaat tgaaaattaa gccgataagg caaaagccgg gcagaagagc
    10321 aacatcttta atctgacccg gctttttagt ttgtcaggtt ggcgattaac gcggattgtt
    10381 ttttgaaaag cgcagttttt ataaatgttc gaagaattga aataaaagtc gtcggcaata
    10441 agatttccgt atgaaatgct caaaaacttt tcatttggag cggcgaaact tgaccttttc
    10501 gcccgtcgaa ttataattga gtattcgtgg ctgggtagct cagctggtta gagcgcggga
    10561 ttcataaccc cgaggtcggg agttcaagtc tcccccccgc caccacattt tccagataaa
    10621 aagtttaagg gagcaagcct gctcctttta tttttttaac cgggcatttc agcaggtaat
    10681 tttacaaaat ttccctgtga gctttatata cgggcaaggc ttacaaagct tttcgtgtta
    10741 agatattcgc catgaagatt tgtcccaaat gtcactcttc atattccgat gaaactctga
    10801 atttttgctt gacggacggc gttccgctgg ttgccgaaga ggcttacggc gggcggcttt
    10861 ccagccagca ttcgtggcag gaggctgaaa ctctgcacga cagcaatttc gttttcccgt
    10921 cgacgggcga gcacacgact tcgccgaatt ccggttcgcc gacctttgct tcgtccgctt
    10981 cgcacaacac ttccatcaat gttattccgc cgaaaaaatc ttcgaatcgc gttttgctct
    11041 cgattgtcgg agcgctttta gcgctcggaa cggcaggcgg cgctttctgg tattttgccg
    11101 gacgagatac aaattcctca aattcgtcag gctcgattcc aacggcgacc gtcgaagcta
    11161 aaaagacttc cggcgcactg aacgccgagc aggaaaatca aatcaaaaaa gaaattaccg
    11221 cgatgatgga gacgtggcgc tcgaccatcg aaaagcacga tatcgacacg cacatcaaat
    11281 cctatgtcgc cacgctggac aattattaca aggaaagcag cgtccaccga gagcacgtcc
    11341 gcgccgaccg cctgcgcgcc ttcgagcgct acccgactat taatctccag attgataata
    11401 taaaaatcac gcctcattcg aatgtttcgg caaccgtcgt tttcgataaa acctggacgt
    11461 ttaaaagacc ggacagaacc tcgaccggct ccgttcaaca ggagatggag ctcgtcaaac
    11521 aggacggcag atggctgata aacg
//
LOCUS       DASWNE010000159         7616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_146003, whole genome shotgun sequence.
ACCESSION   DASWNE010000159 DASWNE010000000
VERSION     DASWNE010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7616
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146003"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..1472)
                     /locus_tag="VGB68_03295"
     CDS             complement(39..1472)
                     /locus_tag="VGB68_03295"
                     /inference="COORDINATES: protein motif:HMM:NF041895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor V domain-containing protein"
                     /protein_id="HEY0048285.1"
                     /translation="MQTRKLILLKLSVIGLVAIIAAIGFFGSFNNFNKAKASASGPTP
                     GHTGAPGEANCTACHSDAPVNSGTGSITIGGIPANYRPNQQIPITVTTSQETGVTFGF
                     QLTAVDGQGRSVGTFTLPQQNPPQMQIVEGIINGNIRRYVQHTSDGIVPTQFGFKTWN
                     FTWTAPGTRVGRVGFHAAGNGANSDGGPNGDYIYTTSRSAFAGTLISNFDGDTKSDIT
                     VFRPSAGSWYSLNSSNGQFVATQFGAAGDKIVPGDYDGDGKADLVVWRPSDGTWYLLR
                     SQAGFTAVQFGSNGDLPVSGDFDGDLKTDLTVFRPSTGAWYRINSSNNQFVATQFGAN
                     GDKPAHGDYDADGKTDVAVFRPSNGYWYILRSTDSQFLAIQFGASGDKPAPGDYDGDG
                     KTDQVVFRPSDGAWYLLRSTAGFTAFQFGISSDIPAAADYDGDGKTDVAVYRSGAWYI
                     LPSTGGFTAVNFGIAGDIPAPAGYIAE"
     gene            1796..2917
                     /locus_tag="VGB68_03300"
     CDS             1796..2917
                     /locus_tag="VGB68_03300"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY0048286.1"
                     /translation="MGQGAGWLFAVKIILYLLGFSALVLTVLPFLRLSAWWIRIGEFP
                     RLQIAVVAVLVNIVFNIVGYPLRLVEVVFLTALSACAVYQLFCILPYTPFYRKQVQKS
                     RVPLPKKVIRILFSNVFIENRQYEKVLRMVEKTNADIVLLAETDEDWLKAVEPLKKLY
                     PTMIAHPLDNGYGMVLFSRLKLINPELKFIIEDVVPSIHTEVELRSGDIVRLYCMHPR
                     PPIPMETGRSTERDAELLLVGREIAEHDKQDKPTIIFGDLNDVAWSRTTRLFQKISRL
                     LDPRVGRGMYSTFPVKYPLLHFPLDHVFHTDHFRLVELRRLKSVGSDHFPVYVALSLE
                     TTARFVQEAPEADADDHAEAHETIQEALELLEQEKRADN"
     gene            3208..4326
                     /locus_tag="VGB68_03305"
     CDS             3208..4326
                     /locus_tag="VGB68_03305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEY0048287.1"
                     /translation="MRKYLKFILLLLFAVFIFWFFGRNLNWDEVGQSLRQADATYLTV
                     AVLIICLGYLLRAKRWQVLLAPITDTSLKELFATTTVGFAAVFFIGRMGEIVRPMWLP
                     MRDRRVRPSAALVTLGVERICDLTALICFFAANLVWFSAPAGREAEFASVKIVGILML
                     IGVAFGIALLALYARFSEPIIEWFKRATDRKFIPSRIRRIFLSILKQLAAALRVFKDW
                     REVVSVVFWTLALWFAIAVPTWLVLLAFNLPLDFSASLFIMGFAAVSSVVPTPGGAAG
                     AFHTATAASLIFLNIDRNVAGATSIAMHLVYFAPAVIFGLYYFFHGDISIAKLRNLLS
                     SEHAVEEVEHENDARVESLDSRIQSPNSKVNLNFESQV"
     gene            4371..4880
                     /gene="nrdR"
                     /locus_tag="VGB68_03310"
     CDS             4371..4880
                     /gene="nrdR"
                     /locus_tag="VGB68_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEY0048288.1"
                     /translation="MRCPFCGFLEDKVVDSRESKDGDSIRRRRECLECSRRFTSYERI
                     DEIPYMVVKKDGRREPFARHKVMSGLLRACEKRPISAAQLESIVDAVEKTVQDSPDRE
                     LSTSEIGKVIMRRLKELDKVAYVRFASVYLEFEDVSEFMSELKDLVRSRDKAQTSRHP
                     KTRKAARKK"
     gene            5045..7474
                     /gene="lon"
                     /locus_tag="VGB68_03315"
     CDS             5045..7474
                     /gene="lon"
                     /locus_tag="VGB68_03315"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:NF008053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEY0048289.1"
                     /translation="MDEIEDLTMQVGEAEGGEPQLQIPAELPVLPLRDIVIYPFMIVP
                     LFVSRDRSIKAVDEALSQNRMILLVSQKDVDKEEPTQEDLYKVGTVAVIMRMLKLPDG
                     RIRILIQGLSRALVDSVTVGSDYIKAQVTPINEPMITSNTLEVEALVRNVRASMERAA
                     SLGKNISPEVLAIIANLEDAGRLGDLSASNLELKVEDAQSVLDISDPIPRLRRVNELL
                     SKEIDVLTVQQEINTQARADIDRSQREYFLRQQLKAIQGELGEGNELYEEIEQYRDKI
                     LKAEMPQPAEEEALRQLKKLERMHPDTAETATLRNWLDIMTDLPWSRASKDNLDLKKA
                     QKILDEDHYGLERVKERIIEALAVRKLKEKPKGSILCLVGPPGVGKTSLGRSIARALN
                     RKFVRLSLGGLHDEAEIRGHRRTYVGAMPGRIIQGIQQAGTNNPLVILDEIDKVGADF
                     RGDPSSALLEVLDPEQNSSFRDNYLGVTFDLSNVMFMTTANVLDTIQPPLRDRMEIIS
                     LSGYTEEEKLEIARRHLLPKQLDENGLQKTDVSFSKTAIAGIIGQYTQEAGLRQLERE
                     IGKVCRKVARKKAELEDAFEIVKITRENLHEFLRAPKIFGETALKKDEIGIVTGLAWT
                     AVGGDILFIEAIRMKGKGNLVLTGQLGEVMQESARAAFSYAKARASELDIDEDDFEKY
                     DIHVHIPEGAIPKDGPSAGITMATALVSVLSRRPVRKEFAMTGEITLRGNVLPVGGVK
                     EKILAARRARVKKVILPEPNKRDLEDLPQEVLRDLTFIFVDNVRQVFREVLLEKELPV
                     EKSKRASAKQA"
BASE COUNT         1815 a         1930 c         2056 g         1815 t
ORIGIN      
        1 ttattgaaga acaaacgctc aggcgcgctt tatcgatttt attccgcgat atatccggcg
       61 ggcgctggaa tatcgcccgc gattccgaaa ttgacagccg taaaaccgcc ggtgctcggc
      121 agaatatacc aagcgccgct gcgataaacg gcgacgtcgg tttttccgtc gccgtcgtaa
      181 tcggcggccg cgggaatatc ggaactaatg ccgaattgga aagcggtaaa tccggcggtg
      241 cttcgcagca gataccatgc gccgtctgac gggcggaaaa cgacctgatc ggttttgccg
      301 tcgccgtcat aatcgcccgg cgcgggtttg tcgccgctcg cgccgaactg aatggcgaga
      361 aactggctgt cggtgcttct caatatatac cagtaaccgt ttgacgggcg aaacaccgcg
      421 acgtccgttt tgccgtccgc gtcgtaatcg ccgtgcgcgg gcttgtcgcc atttgcgccg
      481 aactgcgtcg ctacgaattg gttgttggag ctgtttatgc gataccacgc gccggtcgaa
      541 ggacgaaaca cggtcaaatc ggttttcaaa tcgccgtcga aatcgccgct gacgggcaaa
      601 tcgccgttgc tgccgaactg cacggcggta aatcccgcct gacttctgag cagataccag
      661 gtgccgtccg aaggtcgcca gacgaccaaa tcggctttgc cgtcgccgtc gtaatcgccc
      721 ggcacgattt tatcgcccgc cgcgccgaac tgcgtggcga cgaattgacc gttcgagctg
      781 ttcaggctat accacgaacc ggcggaagga cgaaaaaccg tgatgtcgga tttggtgtcg
      841 ccgtcgaaat tggaaatgag cgttccggca aaagcgctgc gcgaagtcgt gtagatataa
      901 tcgccgttcg gtccgccgtc gctgttggcg ccgttgccgg cagcgtgaaa gccgacgcga
      961 ccgacgcgcg tgcccggcgc tgtccacgta aagttccacg tcttaaagcc gaactgcgtc
     1021 ggcacgattc cgtcactcgt atgctggacg tagcggcgta tattgccgtt gatgatgcct
     1081 tcgactatct gcatctgcgg cggattttgc tgcggcagcg tgaaagtgcc gacgcttctg
     1141 ccctgaccgt cgaccgcagt taattgaaaa ccgaaagtga cgccggtttc ctgactggtc
     1201 gtgaccgtaa tcggaatttg ctgattcggg cggtaattgg cgggaatgcc gccgatggta
     1261 atgctgcccg ttcccgaatt gaccggagcg tcgctgtggc aagccgtgca gttggcttcg
     1321 cccggcgcgc ccgtgtgtcc cggcgtcgga ccggacgccg aagcctttgc tttattaaaa
     1381 ttgttaaagg aaccgaaaaa accgatagcg gcgattatag cgaccaagcc gatgacggaa
     1441 agtttcagca aaatcagctt tcgagtttgc atgctcttct aaactcctta attgcttcaa
     1501 aaaattatga cgggatagaa cgactgtttg tttgacgccc ttcatcaaac gaaagtttaa
     1561 gaatttcgta ttcattttac gatagatttt acgcttcgga aagtcaatga attttattta
     1621 atgataagat attaaatgta gttgatagtt gataactgat aactgatagt tgataactga
     1681 taactgaaaa ttaaaagctg aaagctgacg gctgacagct tttcaactat cagttatcaa
     1741 ctatcaacta tcaactatta cggtttgatt tgaagagagc gggaagatgg attgaatggg
     1801 acaaggcgca gggtggttgt ttgcagtcaa gataattctt tatctgttag gattttcggc
     1861 actcgtattg acggttttgc cgtttctgcg gctcagcgcg tggtggattc gcatcggcga
     1921 atttccgcgc ctgcaaatcg ccgtcgtcgc cgttctggtc aatatcgttt tcaatattgt
     1981 cggttatccg ctccggctcg tcgaagtcgt ttttctgacc gcgctttccg cctgcgccgt
     2041 ttatcaactt ttctgcattc tgccttacac gccgttttac cgcaaacagg tacagaaaag
     2101 ccgcgttccg ctgccgaaaa aagtcatccg cattttgttt tccaacgtct ttatcgaaaa
     2161 ccgtcaatac gagaaggtct tgcgaatggt cgaaaagacg aacgccgaca tcgttttgct
     2221 ggcggaaacc gacgaagact ggctcaaagc cgtcgagccg ctgaaaaaac tttacccaac
     2281 gatgattgct cacccgctcg acaacggcta cggaatggtg cttttctcgc gcctgaagct
     2341 tatcaatccg gagctgaaat ttattatcga agatgtcgtg ccgtcgattc acaccgaggt
     2401 cgagctgcgt tcgggcgaca tcgtcaggct ttattgtatg cacccgcgcc cgccgattcc
     2461 gatggagacg ggacgctcga ccgagcgcga cgccgagctt ttactggtcg ggcgcgaaat
     2521 cgccgagcac gacaaacagg ataaaccgac catcattttc ggcgatttaa acgacgtcgc
     2581 ctggtcgcgg acgactcgtc tgttccagaa aatcagccgc ctgctcgacc cgcgcgtcgg
     2641 gcgcggaatg tattcgacgt ttcccgtcaa atatcctctg cttcattttc cgctcgacca
     2701 tgtttttcac acagaccatt ttcgactggt cgagcttcgc cgtctgaaaa gcgtcggctc
     2761 agaccatttt ccggtttacg tcgccttaag tctggaaacg accgccagat tcgtgcagga
     2821 agcgcccgaa gccgacgccg acgaccacgc cgaagcgcac gaaacgattc aggaagcgct
     2881 ggagctgctc gaacaggaga aaagagcgga taactgataa ctgataactg ataactgata
     2941 actgatagtt gataatctct ttaattcttc actatcagtt atccactatc aactatcagt
     3001 tatcgagcta tcaactatcg gttatcgagc tgtctttttc gccgcctgcg tcagactgta
     3061 taaacgaaat tacgataagt ttcggtaaac ttcattcaaa aaatgtgaaa cttattacaa
     3121 ttgacttaaa ttttatggga aaatacggta tcttgtgttt ttatcgtatt gacacacaat
     3181 aaaatcatta tctagtagga aaatattttg cggaagtatc taaaattcat attgctgttg
     3241 ttgttcgccg tttttatatt ctggtttttc ggtcgaaatc tgaattggga cgaagtcggt
     3301 caaagcctgc ggcaggcgga cgctacgtat ctgacggtcg cggttctgat tatctgtctc
     3361 ggctatcttt tgcgggcgaa acgctggcag gtgctgctcg cgccgattac cgacacttcg
     3421 ttaaaagagc ttttcgcgac gacgacggtc ggtttcgcgg cggtattttt tatcggcaga
     3481 atgggcgaaa tcgtgcgtcc gatgtggctg ccgatgcgcg accggcgcgt gcgtccttcg
     3541 gcggcgctgg taactttggg cgtcgagcga atctgcgatt tgacggcgct gatttgtttt
     3601 ttcgccgcga atttagtgtg gttcagcgcg ccggcgggca gggaagcgga attcgcttcg
     3661 gtcaaaatcg tcggcatttt gatgctaatc ggcgtcgcgt tcggcattgc gctgctggct
     3721 ttatacgcca gattttccga gccgattatc gaatggttta aacgcgcgac ggacagaaaa
     3781 tttattccga gccgcattcg tcgaattttt ctgagcattc tgaaacagct tgccgccgct
     3841 ttgcgcgttt ttaaggactg gcgcgaggtc gtttcggtcg ttttctggac gctggcttta
     3901 tggtttgcca tcgccgttcc gacgtggctg gttttgctcg ctttcaatct gccgctcgat
     3961 ttcagcgcgt cgctgtttat aatgggtttt gcggcggtca gctcggttgt gccgacgccg
     4021 ggcggcgcgg cgggcgcgtt tcacacggcg acggcggcga gcttgatttt tctgaacatc
     4081 gaccggaacg tcgccggagc gacttcgatt gcgatgcatc tggtttattt tgcgccagcc
     4141 gtgattttcg gtctgtacta ctttttccac ggcgatattt ccatcgccaa gctgcgtaat
     4201 ctgctctcaa gcgaacacgc ggtcgaagaa gtcgaacacg aaaacgacgc gcgggtcgaa
     4261 agcctggact ccaggattca aagcccgaat tccaaagtca atttgaattt tgaaagtcag
     4321 gtttagacag cggtttcaat cgaaaatcgc aaatctaaaa tcgaaaattt atgagatgtc
     4381 ccttttgcgg ttttctggaa gacaaagtcg tcgattcgcg cgaatcgaag gacggcgatt
     4441 cgattcgccg ccgccgcgaa tgtctcgaat gcagcaggcg ttttacgtca tacgagcgga
     4501 ttgacgagat tccgtatatg gtcgtcaaaa aagacggtcg ccgcgagcct tttgcccgcc
     4561 ataaagttat gagcgggcta ttacgcgcct gcgagaaacg tccgatttcc gccgctcagc
     4621 tcgaatcaat cgtcgacgcg gtggaaaaaa ccgtgcagga ttcgcccgac cgcgagctct
     4681 cgaccagcga aatcggcaaa gtgattatgc gccgcctgaa agaactcgac aaagtcgcct
     4741 acgttcgctt tgcttccgtg tatctcgaat ttgaagacgt ttccgaattt atgagcgagc
     4801 ttaaagattt ggttcgctcg cgcgataaag cgcagaccag ccgccatccc aaaacgcgga
     4861 aagccgcccg aaaaaaataa atcttacatc agtcgtaaac ggtaagcggt aagtggtcag
     4921 ttcgtcgctt tgaatttcga gttttgaata aaaaattcga tattcggcga ttgcaactga
     4981 ccgtttaccg tttaccgttt accactattt cgcgttttcc attcaaatcg gctaacataa
     5041 aattatggat gaaattgaag atttgacgat gcaggtcgga gaagccgaag gcggcgagcc
     5101 gcaactgcaa attcccgctg aactgccggt tttgccgctg cgcgacatcg tgatttaccc
     5161 gtttatgatt gtgcctcttt tcgtttcgcg cgaccgttca atcaaggcgg tggacgaggc
     5221 tctgagccag aaccggatga ttctgctcgt ctcgcaaaag gacgtggaca aggaagaacc
     5281 gacgcaggaa gacctttaca aagtcggcac ggtcgccgtg attatgcgaa tgctgaagct
     5341 gcccgacggg cgcattcgca tcctgattca aggactttcg cgggcgctgg tcgattcggt
     5401 cacggtcggc agcgattaca tcaaagcgca ggtgacgccg attaacgagc cgatgattac
     5461 atcgaacacg ctggaggtcg aggctctggt cagaaacgtg cgcgcttcga tggagcgcgc
     5521 cgcgagcctc ggcaaaaaca tttcgcccga agtgctggcg attatcgcca atctggaaga
     5581 cgccggacgc ctcggcgatt tatccgcttc caatctggag ctgaaagtcg aagacgcgca
     5641 gagcgttctg gatatttccg acccgattcc gcgcctgcgt cgcgtcaacg agcttttgag
     5701 caaggaaatc gacgttttga ccgtccagca ggaaatcaac acgcaggctc gcgccgacat
     5761 cgaccgctcg cagcgcgaat attttctgcg ccagcaactt aaagccattc agggcgagct
     5821 gggcgaagga aacgagcttt acgaggaaat cgagcaatat cgcgacaaga ttctgaaagc
     5881 cgagatgccg cagccggcgg aagaagaagc cctgcgccag ctgaaaaagc tcgaaagaat
     5941 gcatcccgac accgccgaaa ccgcgacctt gagaaactgg ctggacatta tgaccgattt
     6001 gccctggtcg cgcgcgtcga aggacaatct ggatttgaaa aaagcgcaga aaattctcga
     6061 cgaagaccac tacggcttgg aacgcgtgaa agagagaatt atcgaggctt tggcggttcg
     6121 caaattaaag gaaaagccga aaggctcgat tctgtgttta gtcggaccgc cgggagtcgg
     6181 caaaacgtct ctgggacgct cgattgcgcg cgctttaaat cgaaaattcg tgcggctcag
     6241 cttgggcggc ttgcacgacg aagcggaaat tcgcggtcat cgccgcacct acgtcggcgc
     6301 gatgccgggc agaattattc agggcatcca acaggcggga acgaacaatc cgctcgtcat
     6361 tctggacgag attgacaaag tcggcgccga ttttcgcggc gacccttcgt cggcgcttct
     6421 ggaagtgctc gacccggaac aaaacagcag tttccgcgac aattatctgg gcgtgacgtt
     6481 tgatttgtcg aatgtgatgt ttatgacgac cgccaacgtt ttagacacca ttcagccgcc
     6541 tttgcgcgac cgtatggaaa tcatatcgct ttccggctat accgaggaag aaaaactgga
     6601 aatcgcccgc cgccatcttt tgccgaaaca gctggatgaa aacgggctgc aaaagaccga
     6661 cgtctcattc tcgaaaaccg cgattgccgg catcatcggt caatacacgc aggaagccgg
     6721 tctgcggcaa ttggaacgcg aaatcggcaa agtctgccgt aaagtcgcgc gcaaaaaagc
     6781 cgagctcgaa gacgctttcg aaatcgtcaa gataacgcgg gaaaatctgc acgaatttct
     6841 gcgcgcgccg aagattttcg gcgaaaccgc tctgaaaaaa gacgaaatcg gcatcgtcac
     6901 cggcttggct tggacggcgg tcggcggcga tattctgttt atcgaagcca tcagaatgaa
     6961 aggcaaggga aatctcgttt tgaccggaca actcggcgaa gtaatgcagg aatcggcgcg
     7021 cgccgcgttt tcttacgcaa aggcgcgggc atcggaactg gacattgacg aggacgattt
     7081 cgagaaatac gacatccacg ttcacatacc cgaaggcgcg attccgaaag acggtccgag
     7141 cgccggtatt acaatggcga cggcgctggt ctcggtgctc tcccgcagac ccgtccggaa
     7201 ggagtttgcg atgaccggcg agataacatt aaggggcaac gttctgcccg tcggaggcgt
     7261 caaagaaaag attctggcag cccgccgcgc gagagtgaaa aaagttattc tcccggaacc
     7321 gaacaaacgt gatttagagg atttacctca ggaagttttg cgtgatttga catttatatt
     7381 cgtagataac gtgcggcagg ttttccgcga ggtattgctg gaaaaagaat tgccggttga
     7441 aaagtcgaaa cgcgcatccg ctaaacaggc ttaagaacag gtggaaaagc cggtttttaa
     7501 gccggtgatt ttcataatag ccagatttta tcggcattct gaacgttgac acggtctggc
     7561 ttgtatgtta cggttaaaat tccggtttct ataggattca taaacaatcc acaaaa
//
LOCUS       DASWNE010000160         4419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_146057, whole genome shotgun sequence.
ACCESSION   DASWNE010000160 DASWNE010000000
VERSION     DASWNE010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146057"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VGB68_03320"
     CDS             complement(<1..276)
                     /locus_tag="VGB68_03320"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEY0048290.1"
                     /translation="MAENIENTQNTNTGETTRTPGKRFEIPVLPLQNTTLFPDTVVPL
                     NVGRKRSVQAVEAALATEEKLIACISAKSEEVTGNDARPVDLYEVGTL"
     gene            complement(310..705)
                     /locus_tag="VGB68_03325"
     CDS             complement(310..705)
                     /locus_tag="VGB68_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY0048291.1"
                     /translation="MAESIERYFHFLAAPKGSRPSGRLWYPAADVYNTPDGWIVKIEL
                     AGVSADDLEIEIEGSTLYIAGIRRDKTCRGGDVSYHQMEITYSRFEKTLQFPDMIEGA
                     KLEHDYTDGLLFIYLKKGGTEAKTPDKQS"
     gene            complement(828..1295)
                     /locus_tag="VGB68_03330"
     CDS             complement(828..1295)
                     /locus_tag="VGB68_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY0048292.1"
                     /translation="MAENQISANERRRCREIMTHNVTTATREMSLQAVAALMRDGDMG
                     SLPVVENGKLVGIVTDRDIVVRAVAEGREAQAAIGDVMTTEIFALRAEDFVFEAIRLM
                     GDRQVRRVPVINENGELVGIIAMADVALETEDEREIAETLEEISSGAGFWKKS"
     gene            1530..1877
                     /locus_tag="VGB68_03335"
     CDS             1530..1877
                     /locus_tag="VGB68_03335"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY0048293.1"
                     /translation="MGKVYDNRRKPRREGKKITVGKAEAVPAGRGATVQLKGGGELAL
                     FNVGGKFYATENFCPHKGAPLADSRLYGNVVECDLHGWRFDVASGECFTKKSCSIETY
                     EVVVEDGWIKILV"
     gene            complement(1910..2143)
                     /locus_tag="VGB68_03340"
     CDS             complement(1910..2143)
                     /locus_tag="VGB68_03340"
                     /inference="COORDINATES: protein motif:HMM:NF024763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEY0048294.1"
                     /translation="MAEIQDKLPENVRGKFYVDNQCIDCDVCRDTSPKNFTRNDENGY
                     SFVYRQPVTAEELELCKEARDACPVEAIGDDGN"
     gene            complement(2181..3098)
                     /gene="rnz"
                     /locus_tag="VGB68_03345"
     CDS             complement(2181..3098)
                     /gene="rnz"
                     /locus_tag="VGB68_03345"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEY0048295.1"
                     /translation="MSSRVFIALGTASQVPTRERNHNGYFIRWDEEGFLLDPGEGTQR
                     QMIFAGVTASQITKIFITHFHGDHCLGLAGVLQRLSLDRISHEVEIYYPAYGRKFVEN
                     LRNSSVYHNVANISEKPFAGAGVIFENKNLKIETRRLDHSVESWGYRFQEKDDVTMLP
                     EKLKEFGVFRKDIGELKAAGKIEINGKIVLLEQVSVFKKGLSAAFVMDTRVCESAREL
                     AKDVDFLICESTYLAGETADAVKNGHLTATQAAEIAQNANAAKLVLTHFSQRYNSIED
                     FVREAKTVHENTIAVSDGEQVGILRREKA"
     gene            complement(3199..4323)
                     /gene="nadA"
                     /locus_tag="VGB68_03350"
     CDS             complement(3199..4323)
                     /gene="nadA"
                     /locus_tag="VGB68_03350"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006883.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEY0048296.1"
                     /translation="MSAVLEQVRNLEDYLVMPDEEIDERIQSAKQRLGSRVVVLGHHY
                     QRDDVIRHADLQGDSYQLSVMASETDAEYIVFCGVHFMAESADILGKPAQRVILPDMG
                     AGCSMADMATVEQVEDAWEQLREIGVLQQRVAPITYMNSSAAIKAFCGRNGGVVCTSS
                     NAVPLFDVYLKEFDKMFFFPDQHLGRNTGAKFGIPLEKMVLWNPHEELGGNTEEEIRE
                     AKLILWRGHCSVHGRFKPWHVEQIRKQIPGIKVLVHPECMREVVEISDLNGSTSFIIN
                     TVEKAAPGTKWAIGTEVNLVNRLQQRFPDKDIRLLAPDLCMCATMYRIAPQNLAWALE
                     NLVDGNVVNEIIVEDETKHYALVALQRMIDLTEQNAAKKN"
BASE COUNT         1033 a         1174 c         1044 g         1168 t
ORIGIN      
        1 gagcgttccg acctcgtaaa gatcgaccgg gcgagcgtcg ttgcccgtca cttcttcgga
       61 tttggcgctg atgcaggcga taagtttttc ttcggttgcc agagccgctt cgaccgcctg
      121 taccgagcgt ttgcgaccga cgttcagagg caccaccgta tcgggaaaaa gcgtcgtgtt
      181 ctgcaggggc aggactggaa tttcaaaacg ttttcccggc gttcgagtcg tttcgcccgt
      241 attcgtattt tgcgtatttt cgatattttc agccataaca ataaaactca cgcaggataa
      301 atgcaatttt tacgattgtt tatccggcgt ttttgcctcg gttccaccct ttttcaaata
      361 aataaataaa agcccgtccg tgtaatcgtg ttcgagtttt gcgccctcga tcatatcggg
      421 aaactgcaaa gttttctcaa accggctgta ggtaatttcc atctggtgat aggaaacgtc
      481 gccgccgcga caggttttat cgcgccggat tccggcgata tacagcgtgc tgccttcgat
      541 ttcgatttcg aggtcgtcgg cggacacgcc cgccagctct attttgacaa tccagccgtc
      601 cggcgtgttg taaacgtccg ccgccggata ccagagtctg cccgacgggc gcgagccttt
      661 cggcgcagcc agaaagtgaa aataacgttc gattgattca gccataaatc tacaaagatt
      721 ttccgttaaa aataaaaacc tgtttgcgac cggacaaaaa ccgccgcccc gagcagcaaa
      781 aacagtttta gatttccggc taaatatttc aagaattaat atgtcgatta agattttttc
      841 caaaatcccg cgccgctcga aatttcttcc agcgtttcgg caatctcgcg ctcgtcttcg
      901 gtctccagcg cgacgtctgc catcgcgata atgccgacta gctcgccgtt ttcgtttata
      961 acgggcacgc gccgcacttg tctgtcgccc atcagacgaa tcgcttcaaa aacaaaatct
     1021 tcggcgcgga gggcaaatat ttccgtcgtc atcacatcgc cgattgccgc ttgcgcctct
     1081 cgtccttcgg cgacggcgcg cacgacgata tcgcggtcgg tgacgatgcc gaccagcttt
     1141 ccgttttcca cgaccggcag cgagcccata tcgccgtcgc gcatcagcgc ggcgaccgcc
     1201 tgcagagaca tctcgcgcgt cgccgtcgtc acgttgtgcg tcataatttc ccggcagcgc
     1261 cgccgctcat ttgccgaaat ttgattttcc gccatatatc cagcttcttc taaattttct
     1321 cttaaagata ttttgtttta ccgggcgcga gaaagcaagg tgaaaattaa tgccaaacgc
     1381 aaaagattca ggattcggga ttcaggattc gggattcagg attcagaatt acaaatcttg
     1441 aatcttgaat cttgaatctt gaatcttgaa ttaaaatacg tttcgagcaa cactaatttg
     1501 taatttgcaa tctgaaatta aaatttttta tgggaaaagt ttacgataac cgtcgcaagc
     1561 cgcgccgcga aggaaaaaaa atcacggtcg gaaaagccga agccgttccc gccggacgcg
     1621 gcgcgaccgt ccagctgaaa ggcggcggcg agctggcttt gtttaacgtc ggcggaaagt
     1681 tctacgcgac cgaaaatttc tgtccgcaca agggcgcgcc gctcgccgat tcgcgtcttt
     1741 acggaaacgt cgtcgaatgc gaccttcacg gatggcgttt cgacgtcgct tcgggcgaat
     1801 gttttacaaa aaaaagctgc tcgattgaaa cgtatgaagt cgtggtcgaa gacggctgga
     1861 ttaaaatttt agtttaaaat taaaaggaaa gccggcgaca tttatcgggc taatttccat
     1921 cgtcaccgat agcttcgacc gggcaggcgt cgcgcgcttc cttgcaaagc tctaattctt
     1981 cagccgttac gggctgtctg taaacaaacg aataaccgtt ttcgtcgttg cgcgtgaagt
     2041 ttttcggcga agtgtcgcgg caaacgtcgc agtcgataca ttgattatcg acgtaaaatt
     2101 ttccgcgcac gttttccggt aatttgtctt gaatttctgc cactaattaa aatttgcgct
     2161 ctcgtttata gatgcgcgcc tcaagctttt tctcttctta aaatcccgac ctgctcgccg
     2221 tcgctgacgg caatcgtgtt ttcgtgaacc gttttggctt cgcgaacaaa atcttcaatc
     2281 gaattataac gctgcgaaaa atgcgtcaaa accagtttcg cggcgttcgc gttttgcgcg
     2341 atttcggcgg cttgcgtcgc cgttaaatga ccgtttttga cggcgtccgc ggtttcgccc
     2401 gccagataag tcgattcgca aatgagaaaa tcgacgtctt tcgccagctc acgagccgac
     2461 tcgcaaacgc gcgtgtccat tacaaaagcg gcgctcaagc cttttttaaa gacgctgacc
     2521 tgttcgagca aaacaatttt gccgttgatt tcgatttttc cggcggcttt caattcgccg
     2581 atgtctttcc ggaaaacgcc gaactctttt aatttttccg gcagcatcgt gacgtcgtct
     2641 ttttcctgaa aacgatagcc ccacgattcg accgagtggt cgagccgccg ggtttcgatt
     2701 ttcagatttt tattctcaaa aatcacgccc gcgcccgcaa aaggcttttc cgatatgttt
     2761 gcgacgttgt gataaaccga agagttgcgc agattttcca cgaattttcg accgtatgcc
     2821 ggataataaa tctcgacttc gtgcgaaatt ctatcgagcg aaagccgctg cagaactccg
     2881 gctaaaccca gacaatggtc gccgtgaaaa tgcgtgataa aaatctttgt aatctgcgaa
     2941 gccgtaactc cggcgaaaat catctgtcgc tgcgtgccct cgcccgggtc gagcagaaag
     3001 ccttcctcgt cccaacgaat aaaataaccg ttgtgattgc gctcgcgcgt cggaacctgg
     3061 ctcgccgttc ccaaagcgat gaatactctc gaagacattt ttatgtcgtg aatcgtaaat
     3121 cgtaaatgcc gccggtttgc tgttagctaa gctcgaattt aattcaaatt ttaataacta
     3181 tttacgaatt acgattttct agttcttctt cgccgcgttt tgttcggtca aatcaatcat
     3241 tcgctgaagc gcgacgagcg cgtaatgctt tgtttcgtct tcgacgatga tttcgtttac
     3301 cacatttccg tcaacgagat tttccaatgc ccacgccagg ttctgcggcg cgatgcgata
     3361 catcgtcgcg cacatacaca aatccggcgc gagcaggcga atgtctttgt ccggaaagcg
     3421 ctgctgaagg cgattgacca gatttacttc cgtgccgatt gcccatttcg ttccgggcgc
     3481 ggctttttcg accgtgttga tgataaacga ggtcgagccg tttaaatccg aaatctcgac
     3541 gacttcgcgc atacattcgg gatgcacgag gacttttatg ccgggaattt gcttgcggat
     3601 ttgctcgacg tgccacggtt taaaacgccc gtgaaccgag caatgcccgc gccacaaaat
     3661 caatttcgcc tcgcggattt cttcttccgt gttgccgccg agctcttcgt gcgggttcca
     3721 caaaaccatt ttttcgagcg gaatgccgaa tttcgcgccg gtgtttctgc ccagatgctg
     3781 gtcgggaaag aaaaacatct tgtcgaattc tttcagataa acgtcaaaca gcggaacggc
     3841 gtttgacgaa gtgcagacca cgccgccgtt tcgcccgcaa aaggctttta tcgccgcgct
     3901 cgaattcata taagttatcg gcgcgaccct ttgctgtaaa acgccgattt cccgaagctg
     3961 ttcccaggcg tcctcgacct gttcgacggt cgccatatca gccatcgagc agcccgcgcc
     4021 catatccggc aaaatcactc tctgggcggg cttgccgaga atatcagccg attccgccat
     4081 aaaatggacg ccgcagaaaa cgatatattc cgcgtccgtc tcagacgcca tcacggataa
     4141 ctggtaagaa tcgccctgta aatcggcgtg ccgaataacg tcgtcgcgct ggtaatgatg
     4201 accgagaacg acgacgcgcg agccgagccg ctgctttgcc gactgaatcc gctcgtcgat
     4261 ttcttcgtcc ggcataacga gataatcttc gagatttctg acttgttcca aaactgctga
     4321 catttcctta aatcctttca gtctttagat gcgcgtataa tgtcttgcgt aaaccagaat
     4381 cattacactt tgagaaattt ttcgcaaatt actttgcct
//
LOCUS       DASWNE010000161         4403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_5420, whole genome shotgun sequence.
ACCESSION   DASWNE010000161 DASWNE010000000
VERSION     DASWNE010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5420"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..697)
                     /locus_tag="VGB68_03355"
     CDS             complement(152..697)
                     /locus_tag="VGB68_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048297.1"
                     /translation="MKLRNALLIWMAVFGIAVSLGVPLGTWASHRWGCWKYADATINW
                     YNGGTGDYFNIYNEEARTDSNSWHNYTDLNLVPSAAGTTDHVNAYNGFYGSTGWLGLA
                     EIRGYSGCTVRNGRARLNQSYLDNGSYSRTNKKHVACQEIGHLFGLEHNRGSSTTCMN
                     DTILTAPQPNTHDRDLINSIY"
     gene            1747..2769
                     /locus_tag="VGB68_03360"
     CDS             1747..2769
                     /locus_tag="VGB68_03360"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0048298.1"
                     /translation="MTSSANIKTKSNVELFAKHSDMVHAATTAKSLTTEAAACCLCET
                     DDAEIIGAGQDFEYGTSADVFLARCCRSCGLVYLNPRPAAEEFARIYPPDYHAFEFSE
                     KQFGFVFKVRRRLEARRLLSWCKNLPENARLIDVGCGDGFHLELLRDFGQKNWTLEGI
                     DADSRAVSVAEKKGLKVHYGLLENLDLPSKTYDLAFLLQTIEHVASPVELLREVRSIL
                     RPGGRVVIVTDNTDSLDFKLFKKRYWGGYHFPRHWNLFNRRTIRRLAEKTEMQVESLT
                     TQVSPVNWVYSIRNRLTDKQMPSWLVEQFSLKTPLSLGVFTVFDLVNNLAGRGALLNA
                     ILRRPL"
     gene            2779..4239
                     /locus_tag="VGB68_03365"
     CDS             2779..4239
                     /locus_tag="VGB68_03365"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0048299.1"
                     /translation="MNSKTNKPVVILGAGLAGLTAANRLRKENVPVTLYEAGNKIAGL
                     AQSFNDSDGFTYDFGAHFVTNRLAKAVGVMDKCRTVRHYAETVWLGGKSYSHPFGLMR
                     IPRMTTSGISAKIKSLSSGENPESAADWFRAQYGAALADEVAIPLTEAWSGAKAADLS
                     PAVGDGISSGIARTLWLKAASSVTRRAVACGYSRELPEKASVYHVYPEGGIGTLCSKL
                     AENLDDVIRLESPVEAIIVEDNKVVGVRVNGKIQEASAVISTAPVHVLGKIVEGTDRL
                     SHLARFRYRPMIFVNLRFQGRGLLPDVVTWTPEDEFPFFRLTETPLSMPWLAPEGKTI
                     ITVDIGCEKGDEFWAMDDETLGELCVENLKRIVPDAHSRYLGCRVMKTPIAYPVFLRE
                     YEAERRAFAEGTGVENLHSVGRNGEFMHIFMEDVHHRTERKMREMLSLSDQKSVITAT
                     PSPLSRERQIAATLTVSATRQNEPRVLNEKALPVQD"
BASE COUNT         1063 a         1102 c         1189 g         1049 t
ORIGIN      
        1 aaatttcact gctgctgctg ctgtttacgg cttgctcaac ggtttgatgt tgatgccagt
       61 taatcgtggc ggaaaccgac aaaccgaaaa ccgccgctaa aaacgttcct aacacaaaat
      121 gcttgatttt cattttcgct gtttcctcct tctaatagat tgaattgatt aagtcgcggt
      181 cgtgcgtgtt cggctgcggc gcggtcagga ttgtatcgtt catacaggtc gtgctcgacc
      241 cgcgattgtg ctccagaccg aaaagatgcc cgatttcctg acaggcgacg tgctttttgt
      301 tggtgcgcga gtaactgccg ttatcgagat aactctggtt caggcgggcg cgaccgtttc
      361 tgaccgtgca gccgctgtag ccgcgaattt cagccagccc gagccagccc gtcgagccgt
      421 aaaagccatt gtaagcgttg acgtggtcgg ttgtgccagc cgcagacgga accaggttaa
      481 ggtccgtgta gttatgccag gaattcgagt cggttctcgc ttcctcgttg taaatgttga
      541 aataatcgcc cgttccgccg ttataccagt taatcgtggc gtcggcgtat ttccagcaac
      601 cccagcgatg tgatgcccac gttccgagtg ggactccgag agagacagcg ataccgaaaa
      661 ccgccatcca gatgagtaat gcgtttctta gtttcattct tttccctcct ctttcagaaa
      721 agtgttagct ttggttgaat gtgtcgtttt ttggacacgg tgaatttcaa tggacgctac
      781 tgccgttgac tcttgactaa agatgaccgg acacaattat ccggctaggc aagcagtcgt
      841 gaatttaaag ctagaagata accaaacgcg gcgtttggtc taataggatg ataagtaaaa
      901 tataccttac aagggtatcg tgtcaagaaa aatttttaag ggcgatttgg cattcaattc
      961 agcgtcgcac ctaatgagcg ccttgagcta tcgaaggcaa tatacagttt agccgctaac
     1021 ggtcattaat tttcttgtca agctgaataa tctgttataa atttctcttt aagcctttat
     1081 gcggaaggcg ttccgcaaat gatgcattca agctgatttt tagcagaacc ggttaatagt
     1141 ccggctcggc tgaagttggt aatttatttt tagcgcagtt gtcatccgct aagtgacggc
     1201 ggcggtcaaa ttttattttt cagggaggat atatgaagat ttcaaaactt gtaacgggat
     1261 ttgcggcggc attagttatc gctttcggtt ctattgttta cgtaaattcg ggttttgcga
     1321 gtaacaacgc ttgcgaaaca ggtcaggatt tagcggcgtc ggcgtgcggc aaatgcggcg
     1381 acggctattg caatccccgg tgcggcgaaa cggcaacgag ttgtccgcga gattgcgccg
     1441 gagtgccatc agccagataa tccgctgaat ttagacggtc acaccagtaa aaagccggaa
     1501 gctgagcttc cggcttttta ctgtaattag ggacaaatta gagacaatga cgggcagaaa
     1561 tctccgcgta attgttaaag gcgagaagct tttaataggg ctatcgtaaa aataatcgca
     1621 gattaacgga aaccagggca aattaaaagt tttataaagg cggagcatcg accgtaacta
     1681 cgtgacgata ctaaattttt cacaaaatac cgacccggaa gatatcaagt caatccgcaa
     1741 agtcaaatga catcatcggc aaacatcaaa accaaatcaa acgtcgagct tttcgccaaa
     1801 cattctgata tggttcatgc cgccacaacc gcaaaaagct taacaacgga agcggcggcg
     1861 tgttgtttat gtgagacaga tgacgccgaa ataatcggcg cgggacaaga tttcgagtac
     1921 ggaacgagcg cggacgtttt tttggcgaga tgctgccgct cgtgcggttt ggtatatctc
     1981 aatccgcgtc ccgctgccga agaattcgcg cgaatttatc cgcccgatta tcacgccttc
     2041 gagttttccg aaaagcagtt cggttttgtc ttcaaagtcc gccggcggct cgaagccagg
     2101 cggttattga gctggtgcaa aaatctcccg gaaaatgcac gcctcattga tgtcggctgc
     2161 ggcgacggat ttcaccttga gttgctgcgt gatttcggac agaaaaactg gactctcgaa
     2221 ggtatcgatg cggactcccg cgccgtttcc gtagcggaaa agaaaggctt aaaagttcat
     2281 tacggcttgc tcgaaaatct ggatttgccg agcaagacat acgatttagc ttttctgctt
     2341 caaaccattg agcacgtcgc cagcccggtc gagcttctgc gcgaggtgcg ttcgattttg
     2401 cgtccgggcg ggcgcgtcgt gattgttacc gacaacaccg attccctgga tttcaagctg
     2461 tttaagaagc gatattgggg cggttatcat tttccgcgcc actggaattt attcaaccgc
     2521 cggacgatac gccgcctggc ggaaaaaacc gaaatgcagg tcgaaagcct gacgacgcag
     2581 gtcagcccgg tcaattgggt ttattcgatt cgcaaccgtt tgaccgacaa acaaatgccg
     2641 tcctggctgg tcgaacagtt cagtcttaaa actccgctct cgctgggcgt ttttaccgtc
     2701 tttgatctcg tgaacaatct cgccgggcgc ggcgcattgc tcaatgcgat tctgagacgc
     2761 ccgctttaac taaaaaatat gaattcaaaa acaaacaaac ccgttgtcat tctcggagcc
     2821 ggacttgccg gtctgactgc cgccaatcgt ttgcgaaaag aaaatgttcc ggtcacgctt
     2881 tacgaagccg gaaacaaaat cgccggactc gcccagagct tcaacgactc cgatggattt
     2941 acctacgatt tcggcgcgca tttcgtcacc aatcgcctgg cgaaagccgt cggcgtgatg
     3001 gacaaatgcc gcacggtcag gcattacgcc gagaccgtct ggctcggcgg caaaagctac
     3061 agccatccgt tcggcttgat gcgcatcccg cgcatgacga cgagcggaat cagcgcgaaa
     3121 ataaaatcct tatccagcgg cgaaaatccg gaatcggcgg cagactggtt tcgcgctcaa
     3181 tacggcgcgg ctctggcgga cgaagtggcg attcctttga ccgaagcgtg gtcgggagcc
     3241 aaggcggcgg atttatcgcc ggcggtcggc gacggcattt cgagcggcat cgcccggact
     3301 ctctggctga aagcggcatc cagcgtgacg cgccgcgccg tcgcctgcgg ctacagccgc
     3361 gagctgcctg aaaaggcgag cgtctatcac gtttacccgg aagggggcat cggaactttg
     3421 tgcagcaagc tggcggaaaa tctggatgat gtcatccggc tcgaatcgcc ggtcgaagcg
     3481 attatagtcg aagacaacaa ggtggtcggc gtgcgcgtta acgggaaaat acaggaagcc
     3541 tccgcggtca tcagcaccgc gccagttcac gttttgggaa aaatcgtcga gggcacggac
     3601 cggctctcgc atctcgcgcg ctttcgttat cgaccgatga ttttcgtcaa cctgcgtttt
     3661 caggggcgcg gtctgcttcc ggacgtcgtc acctggacgc cggaagacga gtttccgttt
     3721 ttccgcctga ccgaaacgcc gctttcgatg ccgtggctcg ctcccgaagg caaaaccatt
     3781 atcaccgttg acatcggctg cgaaaaaggc gacgagttct gggcaatgga cgatgaaact
     3841 ttaggcgagc tgtgcgtgga aaacctgaaa cggattgtgc cggatgcgca ttcgcgctat
     3901 ctgggctgcc gcgtcatgaa aacgccgatt gcgtatccgg ttttcctgcg cgaatacgaa
     3961 gccgagcgcc gcgcatttgc cgaaggcacg ggcgtcgaaa atcttcacag cgtcgggcgc
     4021 aacggcgaat ttatgcacat ctttatggaa gacgttcatc accggaccga gcgaaagatg
     4081 cgcgagatgc tcagtctttc cgaccagaaa tcggtcatta cggcgacgcc ttctcctttg
     4141 agcagagagc ggcaaattgc ggcgactctc accgtctcgg caacccggca gaacgaacct
     4201 cgcgttttaa acgaaaaggc gctgcccgtt caggactaaa ggagattttg aaaacaatga
     4261 gtaaccacag gcaagcgaat actaacaggg aagcggcgat gttgatgcaa ggcggtctgg
     4321 tgtggctggg actggctggc ggaggattta tcgtttggct ggcttgtgcg gcaatcgttt
     4381 acctggctct gcccgaaaat atc
//
LOCUS       DASWNE010000162         3741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_392188, whole genome shotgun sequence.
ACCESSION   DASWNE010000162 DASWNE010000000
VERSION     DASWNE010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392188"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1618
                     /locus_tag="VGB68_03370"
     CDS             <1..1618
                     /locus_tag="VGB68_03370"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding/OmpA
                     family protein"
                     /protein_id="HEY0048300.1"
                     /translation="LTPLAKGFITLVILGVLGFVTWYWLGGSIKNWATSGGIGGGGQI
                     ENKDFDPLKNKINDPNYNQGSTGVTAAQIGTGKLNRPLVVAINTWAGHAPGIVYNNGL
                     DVNPSSFYKRNFGMDVKFVVIEDPAAKLAAFLKGDVDIMWDTVDNWAREASTLAERNQ
                     TAKSIIMQDWSRGGDGIVSLSSIKSIEDLKGKKIACTQFTPSHFLLLYLLSQSGLSPE
                     DRSAVEKNIIFTQDAALAAAAFRSKNVDAAVTWEPDLSSAVEARGGEAHVLVSTAAAT
                     NVIADTLVARQDLIDKSPETVRDFINGWFDGIEMMRSNPNPANEVVARALKLDAETVS
                     GMLSGLKLTPYADNAQFYGLTGAKPHYETLFDTAFVIWRKKGLVNKAVAAKDWADSRF
                     VSNLATKYPGQKVEEKQVVAKAPSKEDVPILQQQIQIQFTPGSDEIMPGSYFLLDKLG
                     ELMTSFGGTVLRVEGNTDSTGAASANATLSEKRAISVRNYITKNFPNIQPSRFQTIGR
                     GSTNPVAPNETEAGRQMNRRTDIKVILSNE"
     gene            1709..2491
                     /locus_tag="VGB68_03375"
     CDS             1709..2491
                     /locus_tag="VGB68_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0048301.1"
                     /translation="MFELRQPLPRSRALLISFVAPILLIVVWCALTYGNLVSPDTLPT
                     PTEVIRGTLQLFIEHDLASSIWISAKRIGLAFLLASLVAMPLGVLMGAFETVNRFFEP
                     IVAPLRYMPISAFIPLLIWYFGIYESQKIAFLFLGVFVYLLPVVVFAIRAVPEELVQT
                     ALTLGASKFQVIYSVLVPAALPDIFDSFRVMNAILWTYVILAEAVNPEHGLGYMVELA
                     RTHQKVSWSFAGLIVIGGIGLLTDFLIRAISNLLFRWREKTF"
     gene            2584..3423
                     /locus_tag="VGB68_03380"
     CDS             2584..3423
                     /locus_tag="VGB68_03380"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0048302.1"
                     /translation="MEEQTATASAPAETPSAKAETSPLKLSVKNLFVTYIGRDGEKTE
                     AVRDVSFDIADKPDYGEIIVFLGPSGCGKSTILKSIAGLIAPTKGEILVDGKPVTGVG
                     RDRGMVFQAYTSFAWLTVRKNVEYGLKLQGVDKEKRRELSHKILAEVGLADFAERYPK
                     DLSGGMKQRVAIARTLINNPRIVLMDEPFGALDPQTRWGMQSLLLQISKAENNTIIFV
                     THDVSEAVYLADTCYVLSKRPAQILHRVDVPQFPTRDITLKSSAEFRAVENQLLQMLY
                     NKS"
     gene            3435..>3741
                     /locus_tag="VGB68_03385"
     CDS             3435..>3741
                     /locus_tag="VGB68_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048303.1"
                     /translation="MQKERFQNQRQSKRNFADSLESTFTSEIAGEATATEEKFPEKPS
                     YLKYTFANPYNLTLLGGALVASVLTLNPFIAIGALGLEGLWLLHGSQSRFMQRLLWDP
                     "
BASE COUNT          853 a          958 c         1026 g          904 t
ORIGIN      
        1 attaacaccc ttagcaaaag gttttattac acttgtaatt ctcggcgtgc tgggttttgt
       61 cacgtggtat tggctcggcg gcagcatcaa aaactgggcg accagcggcg gcatcggcgg
      121 cggcggacaa atcgaaaata aagacttcga cccgctgaaa aataagatta acgacccgaa
      181 ttataatcag ggctcgaccg gcgtgacggc tgcgcaaatc gggacgggca agcttaatcg
      241 cccgctcgtc gtcgccatta acacgtgggc gggacacgcg cccggaatcg tttacaacaa
      301 cggtctggac gtgaatccga gctcgtttta caagcgaaat ttcggaatgg acgtgaaatt
      361 cgtcgtcatc gaagacccgg cggcaaagct ggcggcgttt ctcaaaggcg acgtcgatat
      421 aatgtgggac acggtcgaca actgggcgcg cgaagcctcg actctggctg aacggaatca
      481 gacggcgaaa tccattatta tgcaggattg gtcgcgcggc ggcgacggca tcgtctcgct
      541 gtcttcgatt aaatcaatcg aggatttgaa gggcaaaaaa atcgcctgca cgcagtttac
      601 gccgtcgcat tttctgcttt tgtatctgct ttcgcaatcg ggcttgtcgc cggaagaccg
      661 cagcgcggtc gagaaaaaca tcatctttac gcaggacgcg gcgctggcgg cggcggcttt
      721 tcgctcgaaa aacgtcgatg cggcggtgac gtgggagccc gacctttcga gcgcggtcga
      781 agcgcgcgga ggcgaagcgc acgttctcgt ttcgaccgcg gcggcgacca acgtgatcgc
      841 cgatacgctc gtcgcccggc aggatttgat tgataaatcg ccggaaaccg tccgcgattt
      901 catcaacggc tggtttgacg gcatcgagat gatgcgcagc aatccgaatc cggcaaacga
      961 ggttgtcgcc cgtgctttaa agctcgacgc ggagacggtt tcgggaatgc tttccggctt
     1021 gaagctgacg ccgtatgccg acaacgcgca gttttacggc ttgaccggtg ccaagccgca
     1081 ttacgaaacg cttttcgaca cggctttcgt catctggcgc aaaaagggct tggtcaacaa
     1141 agcggtcgcg gcgaaagatt gggctgattc gcgtttcgtt tccaatctgg cgacgaaata
     1201 tccgggacaa aaagtcgagg aaaaacaggt cgtggcgaaa gcgccgtcca aagaagacgt
     1261 gccgattttg cagcagcaga ttcagattca gtttactccc ggaagcgacg agattatgcc
     1321 gggctcatac tttttgctcg ataaactggg cgaattgatg acttcgttcg gcggcacggt
     1381 tctgcgcgtc gaaggcaaca cggacagcac cggcgcagcc tcggcgaatg ccacgctctc
     1441 ggaaaaacgc gcaatttcgg tgagaaatta catcacgaaa aatttcccga atatccagcc
     1501 gtcgcgtttc cagaccatcg gacgcggctc gaccaatccg gtcgcgccga acgaaaccga
     1561 agccggacga cagatgaatc gccggacgga tatcaaggtg attttgtcga atgagtaaat
     1621 acattagaag tcagaagacg aaggtcagta gacggcataa aaacggcttt ctactggctg
     1681 ctgacttctg actaccgact acttttttat gtttgaacta cgtcaaccgc ttccgcgctc
     1741 ccgtgctttg ttaatcagct tcgtcgcccc gattttgctg attgtcgtct ggtgcgcttt
     1801 aacttacggc aatctggtca gtcccgacac gctgccgacg ccgaccgagg tcattcgcgg
     1861 aacattgcag ctttttatcg agcacgattt ggcaagctcg atttggattt cggcgaaaag
     1921 aatcggcttg gcgtttttgc tggcgtcgct ggtggcgatg ccgctcggcg ttttgatggg
     1981 cgcgttcgag acggtcaatc gctttttcga gccgatagtc gcgccgcttc gatatatgcc
     2041 gatttccgct ttcatcccgc ttttgatttg gtatttcggc atctacgaat cgcagaaaat
     2101 cgcgtttctt tttctcggcg tgttcgtcta tcttttgccg gtcgtcgtct ttgcgattcg
     2161 cgccgtgccg gaagagttgg tgcagacggc tctgacgctc ggcgcgagca agtttcaggt
     2221 tatttacagc gttttagtgc ccgccgcgct gcccgatatt ttcgattcgt tccgcgtgat
     2281 gaacgcgatt ttgtggactt acgtaatttt ggcggaagcc gtcaatcccg aacacgggct
     2341 cggttatatg gtcgagctgg cgcggacgca tcagaaagtt tcgtggtcgt ttgccggatt
     2401 gattgtcatc ggcggcatcg gcttgctgac cgattttctg attcgcgcga tttccaatct
     2461 gctgtttcgc tggcgcgaaa aaacgttttg ataagttcaa ggttcaagct ttaaagttca
     2521 aagttatttt tttttctctg actttaaact ttaaaccttg gactttgaat tttgagcttt
     2581 tatatggaag aacaaactgc aacggcttca gctccggctg aaacgccttc ggctaaagct
     2641 gaaacgtcgc ctttaaaact gagcgtcaaa aaccttttcg tcacttacat cgggcgcgac
     2701 ggcgaaaaaa ccgaagccgt ccgcgacgtc tccttcgaca ttgccgataa gcccgattac
     2761 ggcgaaataa tcgtttttct cggaccttcg ggctgcggaa aatcgacgat tttaaaatcg
     2821 atcgccggtt tgattgcgcc gacaaaaggc gaaattctgg ttgacggaaa acccgtcacc
     2881 ggcgtcgggc gcgaccgcgg aatggttttt caggcttaca catctttcgc gtggctgacg
     2941 gtgagaaaaa atgtcgaata cggtttgaag ctgcaaggcg tcgataaaga aaaacgccgc
     3001 gagctttcgc acaaaattct cgccgaagtc ggcttggcgg atttcgccga gcgctacccg
     3061 aaagaccttt ccggcggaat gaaacagcgc gtggcgattg cgcgaacctt gataaacaac
     3121 ccgcgcatcg ttttgatgga cgagcctttc ggcgcgctcg acccgcagac gcgctggggt
     3181 atgcagagcc tgctgcttca gatttcaaag gcggaaaaca acacgattat tttcgtcacg
     3241 cacgacgttt ccgaagccgt ctatctggct gatacgtgtt atgttttgtc gaagcgtccg
     3301 gcgcagattc tgcatcgcgt cgatgtgccg cagtttccga ccagggatat aacgctgaaa
     3361 tcttcggcag aatttcgcgc ggttgaaaat caattgttgc aaatgcttta caacaagtct
     3421 taaagtcgga gtagatgcaa aaagaaaggt ttcaaaatca aagacagagc aagcgaaatt
     3481 ttgccgattc gctggaaagc actttcacgt ccgaaatcgc cggggaggcg acggcgacgg
     3541 aagaaaaatt tcccgaaaaa ccttcgtatc tcaaatacac tttcgccaat ccttataatt
     3601 tgacgctttt gggcggcgcg ctggtcgctt ccgttttgac cttaaatcct tttatcgcca
     3661 tcggcgcgct cggtctggaa ggcttgtggc tgctgcacgg ctcgcagtcg cgttttatgc
     3721 agcgcctgct ctgggacccg c
//
LOCUS       DASWNE010000163         6318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_499028, whole genome shotgun sequence.
ACCESSION   DASWNE010000163 DASWNE010000000
VERSION     DASWNE010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6318
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499028"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1187
                     /locus_tag="VGB68_03390"
     CDS             <1..1187
                     /locus_tag="VGB68_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048304.1"
                     /translation="QQQEQWRQQQQQQRNEQARQQQQQRNEQSRQQQQEQWRQQQNQR
                     RAEQQNQRQQQQEQWRQQQNQRRVEQQRQQQTQQPQQDQRWEQQRRQRQIQQGQQRDE
                     SQRQQQQQQVWQQRQQQRQIQDQQQRQYEDQRRVQQNQQTQRNGQWQRQQQNQRGDYN
                     RGSRNQSSQQWRQWQQQNNSRWQQRQTLDKQRYRILQQQRRLAQLRYEQRYWERLRRD
                     QMRLQQWQYNNYVNNYRYNRGGSYYYTSQYGAQMLRRAINDGYSEGFQAGRADREDGW
                     GFDYNNAFGYQDASYGYDGYYVGMGEYQHYFREGFRRGYEDGYYSRYRYGRYSNGGYS
                     ILGNILSGILQVVISNNNNDDYDDRYNDDRRYNDNDERYDDDNDRYDNNDDRYDEDED
                     GN"
     gene            complement(1299..1820)
                     /locus_tag="VGB68_03395"
     CDS             complement(1299..1820)
                     /locus_tag="VGB68_03395"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY0048305.1"
                     /translation="MNFQIEQAVEILSQTPSVLTSLLGNLSGEWTMNKEDAESWSAFD
                     VVGHFIHAEETDWIPRAEIILAQGENPIFEPFDRFAQFERSKGKTLSELLETFAALRG
                     RSIAALRGMNITPDQLKLKGIHPELGEVNLEQLLATWVVHDLTHIRQIVTVMAQKYSE
                     NVGAWKEYLSILK"
     gene            1934..2290
                     /locus_tag="VGB68_03400"
     CDS             1934..2290
                     /locus_tag="VGB68_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048306.1"
                     /translation="MKTDSKIQIFLLGFIILIVSASYLSFISKNSNLTIATSAEILEI
                     QPKYSRESDGLSVWWKKQNASVIYRYELNGEPARGETIVSFAAARNLKIGEKVKLCYN
                     PQNHKEIEINCVKVNE"
     gene            2283..3653
                     /gene="der"
                     /locus_tag="VGB68_03405"
     CDS             2283..3653
                     /gene="der"
                     /locus_tag="VGB68_03405"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEY0048307.1"
                     /translation="MSKNITNPLVAIIGRPNVGKSTLFNRLTGSRKSIVGDEPGITRD
                     RIYGEVEWKAKKFELVDTGGIVPDDEAIIPANIFKQASFAINQAQAIIWVLDARAGAI
                     PLDEEIGIMLRNIGKPVFIAANKSESRKVEEESAEFYKFGFGLVPVSAEHGNGIGDLL
                     DEVFAVLNFGEEVEEDEQKEIKLAIIGRPNVGKSSLLNKILGEERVIVSPIAGTTRDA
                     IDTELTVDNQKFLLIDTAGIRRKGKTTEMAEKLSVIMARKSLERADVAIIIIDAVEGV
                     TNLDANIAGYAVEEGCSVIIAVNKWDAVEEKETNTIYEFERSLREAMKFLDFAPIVTI
                     SALTGQRVQKILPLVAQANEARNLRIQTSKLNQFFEDNITQPKSGGAPAPVKGGFSRL
                     HVQFLTQAGIRPPLFILFTSGGGKGGLHFSYLRYIENRLREEFDFFATPIKLVERHKS
                     KGKKEK"
     gene            3800..5269
                     /locus_tag="VGB68_03410"
     CDS             3800..5269
                     /locus_tag="VGB68_03410"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEY0048308.1"
                     /translation="MEVILLLIRLFLFGIFALAGIGKLLDLEGSEKAVKAFGTPAEFA
                     KTFAVALPLAEIVFAVCLLFVGTSWLGAIGALILLLSFIGGMIWQLAQGNAPDCHCFG
                     AIKSEPVSRKSLIRNIIFAILALFLVAQGRENQGLSITGSGDGFSEGNVMSVILGLAT
                     VGLLVAVVFYLKRISEQQTQIMRRIEILELTAHEGGRELVREELIHPEDGFPIGAPAP
                     DFALPDLNGKSVSLENLLAANKPILLFFVSPTCHPCKALVPEIEAWQKELKDKVNFVF
                     VSSGKASENAEKFGGAGLKQILLQKGREVADLYFATWTPTAFLVNADGNVGSEPAFGD
                     VKIRELVEKIKVENEVRFITNGKGQNIGESFPEFSLNDLTGKTLNSSAIRGKKTLVAY
                     WSTTCGYCAQMLEDLREWDKTKAADEPNLLLLSRGEIEKNLELGFQSTVVLDDEREVV
                     QKLGMEGTPSAVLVDENGKIVSQVAVGEKRIWALIGKRK"
     gene            5304..5534
                     /locus_tag="VGB68_03415"
     CDS             5304..5534
                     /locus_tag="VGB68_03415"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEY0048309.1"
                     /translation="MKENVLSEKSFAFAVRVVKAYKYLVEEKKEFVLSKQFLRSGTSI
                     GANSEEAVGGQSKADFISKLAIACKEAGETRY"
     gene            5670..>6318
                     /locus_tag="VGB68_03420"
     CDS             5670..>6318
                     /locus_tag="VGB68_03420"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEY0048310.1"
                     /translation="MKPIDWKVTAQEGKARAGILRTRRSVIETPVFMPVGTQGTVKGV
                     RYEWLENELDARIILGNTYHLFLRPGTEVIRACGGLHKFSTWNRSLLTDSGGFQVFSL
                     TDLRKLTEEGVEFRSHIDGSKRFLSPEISMEIQAALGSEIVMVFDECPPGQADFEATR
                     KSLEMTARWARRSKDKFEELQNAGMDTGFLSEQPAAASGSELSGRQSLFGIIQGAG"
BASE COUNT         1723 a         1495 c         1670 g         1430 t
ORIGIN      
        1 agcagcagca ggagcaatgg cgtcaacaac agcagcaaca gcgcaatgaa caggctcgac
       61 agcagcaaca acagcgcaac gagcaatcgc gccagcaaca gcaggaacag tggcgtcagc
      121 aacaaaatca aagacgcgcc gaacagcaaa atcagcgtca gcagcaacag gagcaatggc
      181 gtcaacagca aaatcaacgt cgtgtcgaac aacaacgtca acagcagacg caacagccgc
      241 agcaagacca gcgctgggaa caacagcgcc gtcaacgcca gattcaacag ggacagcagc
      301 gcgatgaatc acagcggcag caacagcagc agcaggtctg gcagcagcgc caacagcagc
      361 gtcagattca agaccaacag cagagacaat acgaagacca gagacgcgtg cagcaaaatc
      421 agcaaactca gcgcaatggt caatggcagc gtcagcagca gaaccagcgc ggcgattata
      481 accgcggcag ccgcaatcaa tcgtcgcagc agtggcgtca atggcagcag caaaacaatt
      541 cgcgctggca gcagcgtcag acgctcgaca aacagcgtta tcgcattctc cagcagcagc
      601 gccggcttgc ccaactgcgt tacgaacagc gttactggga acgcctgcgc cgcgaccaga
      661 tgcgcttgca gcaatggcaa tacaacaact acgtaaacaa ttatcggtat aatcgcggcg
      721 gcagttatta ctacaccagc caatacggag cgcagatgct caggcgcgcc atcaacgacg
      781 gttattcgga aggcttccaa gccggacgcg cagaccgcga agacggttgg ggttttgatt
      841 ataataatgc cttcggctat caggacgcgt catacggata cgatggttat tatgtcggta
      901 tgggcgaata tcagcactat ttccgcgaag gcttccgtcg cggttacgaa gacggttatt
      961 acagccgcta tcgctacggc agatattcga acggcggata cagcattctc ggaaatatcc
     1021 tttcgggaat tcttcaggtc gtgatttcca acaataacaa cgacgattac gacgaccgct
     1081 acaacgacga ccgccgctac aacgataacg acgagcgtta cgatgacgat aatgaccgtt
     1141 acgacaataa cgacgaccgt tatgatgaag atgaagacgg taactagaaa taatttcctg
     1201 tctctcaaaa aacacccgga tttccaaaga agtccgggtg ttttattttt aaagagaagc
     1261 gaaaatccgg cggcgataaa acgtgttttt taacggaatt atttcaggat tgataaatat
     1321 tccttccagg ctccgacatt ttccgagtat ttttgtgcca ttacggtgac gatttgcctg
     1381 atgtgcgtta agtcgtgaac gacccacgtc gccagcagct gttcgagatt gacctcgccc
     1441 aattcgggat ggattccttt taattttaac tggtcgggcg tgatattcat cccgcgcagc
     1501 gctgcaatgc ttctgccgcg taaagcggcg aaagtttcca gcagctcgct caaagttttt
     1561 cctttcgaac gctcgaattg agcaaagcgg tcgaacggct caaaaatcgg gttttcgccc
     1621 tgcgcgagaa tgatttcggc gcgcggaatc caatcggtct cttcggcgtg aataaaatgc
     1681 ccgaccacgt caaacgcgct ccagctttcg gcgtcctctt tattcatagt ccattcgccg
     1741 gaaagatttc cgagcagcga agttaaaacc gacggcgttt gcgaaagaat ctcgacggct
     1801 tgttcgattt gaaaattcat tttggaaagc tcctttcaag tttgctcaaa gaaatatctt
     1861 acatcttgtg cgattaaatc aaacgtgtaa tcattttcag agattgcgtg cggcgaaaaa
     1921 aacgcgggtt aaaatgaaga ccgactctaa aattcaaatc tttcttctgg gttttattat
     1981 cttaattgtt tcggcaagct atctttcctt catttcaaaa aactcgaatc tcaccatcgc
     2041 gacgagcgcc gaaattctgg aaattcaacc aaaatattcg cgcgaaagcg acgggctgtc
     2101 ggtctggtgg aaaaagcaga atgcttcggt aatctaccgc tatgaattaa acggcgagcc
     2161 ggctcgcggc gaaacgattg tcagtttcgc tgcggcgcgc aatctgaaaa tcggagaaaa
     2221 ggtcaaactt tgttataatc cacaaaatca taaagaaatc gaaataaatt gcgtaaaggt
     2281 gaatgagtaa aaatataaca aatcctctgg ttgccatcat cggacgcccg aacgtcggca
     2341 aatcgacgct ttttaatcgc ctgacgggca gccgtaaatc aatcgtcggc gacgagccgg
     2401 gcattacgcg cgaccgcatc tacggcgagg tcgaatggaa ggcgaaaaaa ttcgagctgg
     2461 tcgatacggg cggcatcgtg ccggacgacg aagcgataat tccggcgaat attttcaaac
     2521 aggcgtcttt cgccattaat caggcgcagg cgattatctg ggttttggat gcccgcgcgg
     2581 gcgcgattcc gctcgacgaa gaaatcggga ttatgctcag aaacatcggc aagccggttt
     2641 ttatcgccgc caataaatcc gagtcgagaa aggtagaaga agaatccgcc gagttttaca
     2701 aattcggatt cggactcgtg ccggtttccg ccgaacacgg caacggcatc ggcgatttgc
     2761 tggacgaggt tttcgccgtg ctgaatttcg gcgaagaagt cgaagaagac gagcagaagg
     2821 aaatcaagct cgccatcatc ggacgcccga acgtcggcaa atcttcgctt ttaaataaaa
     2881 ttctcggcga ggaacgcgtt atcgtctcgc cgattgccgg gacgacgcgc gacgctatcg
     2941 acacggaatt aacggtcgat aatcaaaaat ttctgttgat cgacacggct ggaatccggc
     3001 gcaaaggcaa aacgacggag atggcggaaa agctttccgt cataatggcg cgcaaatctc
     3061 tggaacgcgc cgacgtcgct attataatta tcgacgcggt cgagggcgtg acgaatctcg
     3121 acgccaacat cgccggttac gccgtcgaag aaggctgcag cgtgataatc gccgtcaata
     3181 aatgggacgc cgtcgaagaa aaggaaacga acacgattta cgaatttgag cgttccttgc
     3241 gcgaggcgat gaagtttctc gattttgctc cgattgtgac gatttccgct ttgaccggac
     3301 agcgagtgca gaaaattctg ccgctcgtcg cgcaggctaa cgaggcgcga aacttaagga
     3361 ttcagacgtc gaagctcaac cagttttttg aagacaacat cacgcagccg aaaagcggag
     3421 gcgcgcccgc accggtcaaa ggcggatttt cgcggctcca cgtccaattt ctcacgcagg
     3481 cgggaattcg cccgccgctg tttattttgt ttacttcggg cggcggcaaa ggcggtttgc
     3541 atttttcgta tctgcgctat atcgaaaatc gcctgcgcga ggaattcgac tttttcgcca
     3601 cgccgattaa attagttgaa agacataagt ccaaaggaaa gaaagaaaaa taaacgcgaa
     3661 ggaaagaagg aattaacagg gataaaggag ataaatacaa agtctttaat ttttatccct
     3721 ctttatcccc ttcatccctg ttaattcctt ctttcacgcc ttcgcgccct tcgcgttttt
     3781 gcgctaaaat ttcttacgaa tggaagtgat tctattatta atcaggcttt ttcttttcgg
     3841 cattttcgcg cttgccggaa tcgggaagct gctcgatttg gaaggctcgg aaaaagccgt
     3901 taaagctttc gggacgcccg ccgagtttgc gaaaactttt gccgtcgctt tgcctctcgc
     3961 cgagatcgtt tttgccgttt gtcttttatt cgttggaact tcctggctgg gcgcaatcgg
     4021 cgctttgatt ttgcttttga gctttatcgg cggaatgatt tggcagctgg cgcagggcaa
     4081 cgcgccggat tgccattgtt tcggagcgat taagagcgag ccggtcagcc gcaagagttt
     4141 gattcgcaat attatttttg cgatactggc gcttttcctg gtcgcgcaag gcagggaaaa
     4201 tcaaggtttg agtattacgg gttccggaga cggtttttcg gaaggaaatg ttatgtcggt
     4261 tattttaggt ttggcgacgg tcggtttgct ggtcgccgtc gttttttatt taaaaaggat
     4321 ttccgaacag caaactcaga ttatgcggcg catcgagatt ctggaattga ccgcgcacga
     4381 aggcggcagg gaattggtgc gcgaagaatt gattcatccg gaagacggct tcccgatcgg
     4441 cgcgcccgcg cccgatttcg cgctgccgga tttaaacgga aaatcggttt ctctggaaaa
     4501 tcttctggcg gcaaacaaac cgattttgct ttttttcgtc agcccgacct gccatccctg
     4561 caaagcgctt gtgccggaaa tcgaagcgtg gcagaaggaa cttaaagaca aagtaaattt
     4621 cgttttcgtc agcagcggaa aggcgtcgga aaacgccgaa aaatttggcg gcgcggggct
     4681 taaacagatt cttttgcaga aggggcgcga ggttgccgac ttgtattttg cgacgtggac
     4741 gccgaccgct tttctggtca acgccgacgg aaacgtcggc agcgaaccgg ctttcggcga
     4801 cgtgaaaatc cgcgagctgg tcgaaaaaat caaagtcgaa aacgaggtgc ggtttatcac
     4861 caacggcaaa ggtcaaaaca tcggcgaatc gtttcccgaa ttttcgctga atgatttgac
     4921 gggcaaaact ttaaattcga gcgcgattcg cggcaagaaa acgctcgtcg cttattggag
     4981 cacgacctgc ggatattgcg ctcagatgct cgaagatttg cgcgaatggg acaaaaccaa
     5041 agccgccgac gaaccgaatc tcttgctttt gtcgcgcggc gaaatcgaga aaaatctgga
     5101 attgggcttt caatcgaccg tcgtgctgga cgacgagcgc gaagtcgttc agaagcttgg
     5161 aatggaaggc acgccgtccg ccgttctggt cgacgaaaac ggcaaaatcg tctcgcaggt
     5221 cgccgtcggc gaaaaacgca tctgggcttt gattgggaaa agaaaatgaa tttcaaatta
     5281 cgaattacga atgataaagc cttatgaaag aaaatgtttt gtcggaaaaa agtttcgctt
     5341 ttgccgttag agtagttaaa gcatacaaat atttggtcga agaaaagaag gaatttgttc
     5401 tctccaaaca atttttaaga agcggaacat ctatcggagc aaattcggaa gaagccgtag
     5461 gcggacaatc aaaagcggat tttatctcaa aattagctat tgcctgtaaa gaagccggag
     5521 aaacaagata ttgaattaga cttcttaaag cgactgagta ttttgataat acgcagacta
     5581 aaagtctgct cgatgatgta gaagaattac tcaaaattac aggtagaatt ccaatcacga
     5641 gtaaaagtaa ttcgtaattc gtaattgaca tgaaaccgat tgactggaaa gtgacggcgc
     5701 aggaaggaaa ggcgcgggcg ggaattttgc gaacgcggcg ttcggtaatt gaaacgcccg
     5761 tttttatgcc ggtcggaacg caggggacgg tgaaaggcgt gcgttacgag tggctcgaaa
     5821 acgagctgga cgcgcggata attctcggca acacgtatca tcttttcctg cgtccgggca
     5881 cggaagtgat tcgagcgtgc ggcggtctgc ataaattttc gacgtggaat cggtcgctgc
     5941 tcaccgattc gggcggcttt caggtttttt ctttgaccga tttacgaaag ctgaccgaag
     6001 aaggcgtcga atttcgctcg catatcgacg gaagcaaaag atttttgtcg ccggaaatct
     6061 cgatggagat tcaagccgct ttaggctcgg aaatcgtgat ggttttcgac gagtgcccgc
     6121 cgggacaagc cgatttcgag gcgacgcgca aaagtctgga aatgacggcg cgctgggcgc
     6181 gaaggtcgaa agataaattt gaagagctgc aaaatgcggg aatggacacg ggctttctgt
     6241 cggaacagcc cgctgccgcg agcggctccg agctgagcgg acgacaatcg ttattcggca
     6301 ttatacaggg cgcggggc
//
LOCUS       DASWNE010000164         1635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_40553, whole genome shotgun sequence.
ACCESSION   DASWNE010000164 DASWNE010000000
VERSION     DASWNE010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40553"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="VGB68_03425"
     CDS             complement(<1..724)
                     /locus_tag="VGB68_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY0048311.1"
                     /translation="MNKETSKFPPVVLVLFAVLLWSTGGLFIKMTTLDAFAVNAGRSL
                     FAAITVAIFTYRKGLKLDRFTLLTSLLYAGTLSCFVYANKTTTAANAIFLQYTAPIYI
                     LILSPFILKEKFRFPDLITVVFCLGGMSLFFLETPGAENNLASNIFTGNIAALLSGIF
                     FGLYFVFLRHPRSLANKNPALSVFYGNLLIVLFMLPLLAQNPPQPTGNDFLAILFLGV
                     FQIGIAYVLFTNGIAGGVRSLDA"
     gene            complement(887..>1635)
                     /locus_tag="VGB68_03430"
     CDS             complement(887..>1635)
                     /locus_tag="VGB68_03430"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY0048312.1"
                     /translation="AESFHTENADLKTEVRAVAKMVAKSAKTTECLAVGIVLVSLMTL
                     VQVGFDALKAAEGKVFIDPKQAKKSAKEILAERARDDSQGIFGFLKTVDKKFSISYDE
                     NVDKARFKIIYDEELATAAAKDQSQNWKAMDERRWEGVIPVECRSAACGTCWVGVLGG
                     QEKLSEVSRLERKQVKVFGYNQPEDEKPFLRLACQAKAYGNATIVIPPWNGVFGKKVY
                     GNVEESVLEPATTSAAKLRETISAATNDSK"
BASE COUNT          400 a          418 c          437 g          380 t
ORIGIN      
        1 tggcatcgag cgagcggacg ccgcccgcga tgccgttggt gaataaaacg taagcgatgc
       61 cgatctggaa gacgccgaga aataaaatag cgagaaaatc gtttccggtc ggctgcggcg
      121 gattttgcgc cagcagcgga agcataaaca aaacaatcag caggttgccg tagaaaaccg
      181 acagcgccgg atttttattt gccagcgagc gcgggtggcg cagaaatacg aaatacaatc
      241 cgaaaaaaat accggaaagc agcgcggcga tattgccggt aaaaatattc gatgcgagat
      301 tgttttccgc gcccggcgtc tccagaaaaa acaagctcat tccgcccaga caaaacacga
      361 ccgttatcaa atcgggaaag cgaaattttt ccttcaggat aaagggcgag agaatcagga
      421 tgtaaatcgg cgcggtgtat tgcagaaaaa tcgcgttggc ggcggtcgtc gttttgttgg
      481 cgtaaacgaa acaggagagc gtgccggcgt aaagaagcga ggtcagaagc gtgaagcggt
      541 cgagctttaa accttttcga taagtaaaaa tcgccaccgt aatcgccgcg aaaagcgagc
      601 gtccggcgtt gacggcaaaa gcgtcgagcg tcgtcatctt gataaacagc ccgcccgtgc
      661 tccacagcag aacggcgaac agaacgagga cgacgggcgg aaattttgaa gtttctttat
      721 tcacggaaaa atgaattgaa ccgcagagac gccgaggaaa attcaaagta ggctcggcga
      781 cttgatgaac aggactggcg ggataaatgc tttttccgct cgtgttcttt cgagtatatt
      841 cgcggctaaa ttttcttaag aatctcggcg tctctggtct tgaaaattat tttgaatcat
      901 tcgtcgccgc cgaaatcgtt tcgcgcagct tggcggcgga agtcgtcgcc ggttcgagca
      961 cggattcctc tacgttgccg taaacttttt tgccgaatac gccgttccac ggcggaatga
     1021 caatcgtcgc gttgccgtag gctttcgcct gacacgccag ccgcagaaac ggtttctcgt
     1081 cttccggctg attgtagccg aaaactttaa cctgctttct ttccagacgc gaaacctccg
     1141 aaagcttttc ctgaccgccg agcacgccga cccagcaggt tccgcaagcc gccgaacgac
     1201 attcgaccgg aatcacgcct tcccagcggc gctcgtccat cgctttccag ttttgcgatt
     1261 ggtctttggc ggcggcggtg gcgagctctt cgtcgtaaat aattttgaat ctggctttgt
     1321 ctacgttctc gtcgtaactg atggagaatt ttttatcgac cgttttcaga aagccgaaaa
     1381 tgccctgcga atcgtctctg gcgcgttccg ccaaaatttc cttggcggat tttttcgcct
     1441 gtttcgggtc gataaaaact tttccttccg ccgctttgag cgcgtcgaaa ccgacctgga
     1501 cgagcgtcat taagctgacc agaacaatgc cgaccgccag acactcggtc gttttcgccg
     1561 atttcgcgac cattttggcg acggcgcgga cttccgtttt caaatcggcg ttttcggtgt
     1621 gaaaactttc ggcgt
//
LOCUS       DASWNE010000165         2096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_252874, whole genome shotgun sequence.
ACCESSION   DASWNE010000165 DASWNE010000000
VERSION     DASWNE010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252874"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..438)
                     /gene="rpsM"
                     /locus_tag="VGB68_03435"
     CDS             complement(55..438)
                     /gene="rpsM"
                     /locus_tag="VGB68_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEY0048313.1"
                     /translation="MARVSGVDLPPQKRAQIGLTYIYGIGKSRATAVLEEAQINIDTK
                     IRDLSEDELGRIRAILDAQGEVEGDLRKRVQMDIKRLMDIGCYRGLRHRRSLPVRGQR
                     TSTNARTRKGPRKAAVAKKKAPGKK"
     gene            complement(529..642)
                     /gene="rpmJ"
                     /locus_tag="VGB68_03440"
     CDS             complement(529..642)
                     /gene="rpmJ"
                     /locus_tag="VGB68_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEY0048314.1"
                     /translation="MKVRPSVKKMCDKCKVIHRKGVVRVICDNPKHKQRQG"
     gene            complement(658..879)
                     /gene="infA"
                     /locus_tag="VGB68_03445"
     CDS             complement(658..879)
                     /gene="infA"
                     /locus_tag="VGB68_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEY0048315.1"
                     /translation="MSKEEAIEVTATVLETLPNAMFKVELEGNQHQVLAHISGKMRKN
                     FIRILPGDRVLVELSPYDLGRGRITYRYK"
     gene            complement(949..1809)
                     /gene="map"
                     /locus_tag="VGB68_03450"
     CDS             complement(949..1809)
                     /gene="map"
                     /locus_tag="VGB68_03450"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEY0048316.1"
                     /translation="MLDSRVGAVFSKLSHVLLGKRMIIAKSKKDLDKMREVGELVAEV
                     RENLRALVAIGVTSLELNAAADKMIRERGAIPTFIGYHGFPYAICASVNEQVVHGFSN
                     DTPLKEGDIISIDVAATYNDFVGDTAITVPVGKISDEVAQLIKVTEECLHLAIEKCYP
                     NNRIGDLGYAVQSHAEKFGYGVVRDYVGHGIGRKMHEAPQIPNYGRAGTREKIRAGYC
                     FAIEPMINLGSAETKVLGDKWTVITADGKPSAHFEHTIAVTEEGPEILTLSKEQKAQR
                     NQGNAGSAAA"
BASE COUNT          455 a          563 c          476 g          602 t
ORIGIN      
        1 cattttaaga gcagctttct gctcgacgcc cgaccgcttg ccgctcactt ttatttattt
       61 cttgccgggc gctttcttct tcgctacggc ggctttgcgc ggacctttac gggtgcgcgc
      121 gttggtgctg gtgcgctgtc cgcgaaccgg cagcgagcgg cgatgacgca agccgcgata
      181 gcagccgatg tccatcagac gtttgatgtc catctgcacg cgtttgcgca gatcgccttc
      241 gacctcgccc tgcgcgtcga gaatcgcacg gatgcgaccg agctcgtctt cgcttaaatc
      301 cctgattttc gtatcgatat taatttgcgc ttcttcgaga accgccgtcg cgcgcgactt
      361 tccgataccg tagatataag ttaagccgat ttgcgctctt ttttgtggtg gtaaatctac
      421 acctgataca cgagccatat ttctcctaat ttcggattca ggatttagaa ttcaggattg
      481 aagaaatttg attttaatcc tgaattccga atcttgaatt taaaatctct atccctgacg
      541 ctgcttatgc ttcggattat cacaaatcac gcgcacaacg cctttacgat gaataacttt
      601 gcacttgtcg cacattttct ttacagaagg acgcactttc attttaaatc cctcgcttta
      661 tttatagcga tacgtaattc gcccgcgacc taaatcgtat ggcgaaagct cgactaagac
      721 tctgtcgccc ggcagaatac ggataaaatt tttccgcatt ttaccggaaa tatgcgccag
      781 aacctgatgc tggttgccct ctaattcaac cttgaacatc gcgttcggaa gcgtttccag
      841 aacagtagcc gtaacttcta tcgcttcttc tttcgacata tctgacagtg tcaattaaga
      901 gttattgaca aactatttat gttatctctt tttacacgtc ttttcaagtt acgctgcggc
      961 acttccggca tttccctgat ttcgctgcgc tttttgctct ttcgacaaag tcaaaatttc
     1021 cggtccttct tccgtaacgg caatcgtgtg ctcgaaatgc gcgctcggtt ttccgtccgc
     1081 cgtgatgacc gtccatttat cgcccaaaac tttggtttcg gcgctgccga gatttatcat
     1141 cggctcgatg gcgaaacaat aaccggcgcg gattttttcg cgcgttccgg cgcgcccgta
     1201 attcggaatc tgcggcgctt cgtgcatttt gcgaccgatg ccgtgaccga cgtaatcgcg
     1261 gaccactcca taaccgaatt tttcggcgtg cgactgcacg gcgtaaccga ggtcgccgat
     1321 gcgattattc gggtaacatt tttcaatcgc cagatgcaga cattcttccg tgactttaat
     1381 caactgcgcg acttcatcgc tgatttttcc gaccggaacg gtaatcgccg tgtcgccgac
     1441 aaaatcgtta taagtcgccg ccacgtcaat cgagataata tcgccttctt tgagcggcgt
     1501 atcgttggaa aaaccgtgga cgacctgttc gttgaccgaa gcgcaaatcg cgtaaggaaa
     1561 gccgtgataa ccgataaagg tcggaatcgc gccgcgctcg cgaatcatct tgtcggcggc
     1621 ggcgttcagc tccagcgaag tgacgccaat cgcgaccagc gcgcgcagat tttcgcggac
     1681 ttcggcgacc agctcgccga cctcgcgcat tttgtctaaa tcttttttcg atttggcaat
     1741 aatcattcgc tttcctaata aaacgtggct cagtttactg aaaaccgcgc ccactcttga
     1801 atctaacatt tagtcgctcg tccttagtcg attgtgcttt gtcctttagt tagttgaata
     1861 aaaatcgaaa actcgacttt tattcgaccg gcaaagaacc cgcggcgaac gacaaaggac
     1921 taaattaatt tttccagctc ggcgtaaatt tcctcgacgc cgcgctcgcc gttaattttt
     1981 tgcagccttc cggtttgctc gtaataatcg agaagcggtt tcgtgttcgc ttcataagtc
     2041 gaaagccggg tgcgcacgct ttcctcgttg tcgtcggagc gatgatttaa ttgcgc
//
LOCUS       DASWNE010000166         6215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_605746, whole genome shotgun sequence.
ACCESSION   DASWNE010000166 DASWNE010000000
VERSION     DASWNE010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605746"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VGB68_03455"
     CDS             complement(<1..537)
                     /locus_tag="VGB68_03455"
                     /inference="COORDINATES: protein motif:HMM:NF017539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NACHT domain-containing protein"
                     /protein_id="HEY0048317.1"
                     /translation="MKGNKAESLISVEAKFEEYLNRSYEKHSYIPILIFQNQQKKLED
                     IYIPLTVRKQREKENFLLDTYPKKFLPKYKKILIRDTAGMGKSTLMKKLFLSSLEMQQ
                     GIPIFIELRKLKGDDSITSFIHRELNPINDEFDQEFILNLIKDGSFIFFLDGYDEIAQ
                     ADREKVTQNLHDFISKTGK"
     gene            complement(753..1547)
                     /locus_tag="VGB68_03460"
     CDS             complement(753..1547)
                     /locus_tag="VGB68_03460"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY0048318.1"
                     /translation="MAIVSSSTVTTKFQDILNPFGPEESWKDRTGRGVAVAVIDSGID
                     ANHPQLQGKVVESVEAVSEHKRVLFVPSDSGDSAGHGTACAGIIASIAPDAELYSIKV
                     LGAGGAGDGQAFLAGLEYAIKRKIRLINLSLGTTKPQFFAPLHDLLDRAYQAGCIVVA
                     AANNLPQPSFPSVFSSSLISVSKSEDTNPFNFGFKYGEVIELTAPGVNVHTTWLNGGF
                     RSLTGNSFACPNIVGIIALLLETYPSLTPFQVKSLLYSIARKNQES"
     gene            complement(1551..2318)
                     /locus_tag="VGB68_03465"
     CDS             complement(1551..2318)
                     /locus_tag="VGB68_03465"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY0048319.1"
                     /translation="MSNPNLEEKLQTIIETVDVANALTEPLTQSIRNLLDLSAGSMKS
                     EEASVIIRDGDDGDLCFLVAIGKVADQLQGMKIPSGKGVAGFVYSSGQPVVIADVGQE
                     ASFYAEVDKQTGYNTQTMLATPLRYNGEIIGVLEYVNRLGEPPYESFTPFEMDKAALF
                     AEAIASLVNAYESARLFRDLGAKMLADNKETEFREVRDWLKNLRSTSEHREMIDLAIM
                     VREIASRGEAERRLCKEILEAVLKFSDNSLETSFLNY"
     gene            2718..4454
                     /locus_tag="VGB68_03470"
     CDS             2718..4454
                     /locus_tag="VGB68_03470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY0048320.1"
                     /translation="MKRRVFAAVVLAIAGFVCSGVSAQQPTRQPITQLPKQEVEPFKI
                     GRGTSFSASVPRAPENKSLPKNTNPVGTDQIKRDLAEALEVIGANYVDGKRINYNALA
                     KSSIDSMLRSLDPHSNFYDAAQYREMLEEQRSEYTGIGASIVNYTIDGATDTFITSTF
                     PDSPASRAGLQFGDKIVAVGGVNMKGKSSFYVREQIRGEKGKIVRLTIERAATLKTET
                     VEIRRSVVPQPSIPDAYLLRPGIGYVDLSNGFNYTTEEELETALEDLREQGMTSLILD
                     LRDNPGGILEQAVKVAQKFLPAGNTIVTQRARFAIDHRTWKSTNRNAESFPLVVLVNG
                     GSASASDIVAGALQDYDRALIVGENTFGKGLVQSIINLPLGTGLTLTTAKYYTPSGRS
                     IQRDYSSGNLYDYFRHKASFEERKKSAGKTITGRTIYGGNGIEPDEVVKTSTPVEAQT
                     VLLDPMFFFAREAVAGRFRGLESYKSARPVQYGQRVRPSDFPVSEELFDAFKKFVAQS
                     KSWRISAEQLEVNRKFILTRLRFNLATAAFGNVAANQVLIEDDPQVAKAVELLPRAHS
                     LAQAARKTLEKR"
     gene            complement(4590..>6215)
                     /locus_tag="VGB68_03475"
     CDS             complement(4590..>6215)
                     /locus_tag="VGB68_03475"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEY0048321.1"
                     /translation="TINNCILTGNSAARGGALALHGGQTTINNSTISGNTAQLAGGAI
                     SMTQTFAPPTTLVINNSTISGNKVTQAGSNSGGGGIANLQGSSVTVTNSTISGNSVNQ
                     ASGAPGMGGGILMSSGTGTLTNVTVAENFATTCGGVCATTLKARSSIFANNLANEFPD
                     YGGTFDSQGYNLVEVMPATIIGDQTGNIYGQDPQMAPLAGNGGATLTHALNAGSPAID
                     AADPTNFPSIDQRGFSRPADGNGDGARRADIGAFERNAVPRRNFDFDGDGKADVSVYR
                     PADGTWYLLQSTAGFTGQQFGISIDKIVPADYDGDGRTDIAVYRPSDGVWYLLQSSAG
                     FRALQFGAGDDIPVPGDYDGDGRANPAVFRPSTGAWYIARSAGTPSQNFDTVPFGQNG
                     DKPIAGADFDGDAKADVAVFRPSNGYWYRLNSSSNQFVATQFGVAEDKPVASDYDGDG
                     KADIAVWRPSDGVWYILQSINGAFAPVQFGQSGDIPVPADYDGDGVANLAVFRPSTNS
                     WYIGRAFGSPSQNFDTVNFGVGTDKPVPSAFIP"
BASE COUNT         1431 a         1602 c         1706 g         1476 t
ORIGIN      
        1 tttccccgtc ttggagataa aatcgtgtag attctgcgtg actttttcgc ggtcggcttg
       61 cgcgatttcg tcgtaaccgt cgaggaaaaa gataaagctt ccgtctttaa tcaggttgag
      121 aataaattcc tggtcgaatt cgtcgttaat cgggttaagc tcgcggtgaa taaagctggt
      181 gatggaatcg tcgcccttta atttacgcag ctcgatgaaa atcggtatgc cctgctgcat
      241 ttccagagac gacaggaaaa gctttttcat caaagtcgat ttgcccattc cagccgtgtc
      301 gcgtatcaga atctttttat atttcgggag aaattttttc gggtaagtat cgagcagaaa
      361 attttccttt tcccgctgtt ttctaaccgt gagcggaatg taaatgtctt cgagtttttt
      421 ctgctggttt tgaaaaatca gaatcggaat gtatgaatgt ttttcgtaag agcgattcag
      481 atattcttca aacttcgctt caaccgaaat caacgattca gctttattcc ctttcaaaaa
      541 tttagaaagt ctttgaatct tcggcttgac gaaaatttca acaataggcg caatgaaagg
      601 agcggcgaaa ggtgcaacag ttttggttat ttcgagggcg tccgacattc caaaatctcc
      661 gacgatttat tttttatgaa gtaactgcac aaaaaataac agaaatatat tgcgattggc
      721 aagatttttt ttatttaatg cgggcttttg ctctagcttt cctgattttt gcgggcgatt
      781 gaatagagca gcgatttgac ctgaaacggg gtcaggctcg ggtaggtttc cagcaaaagg
      841 gcgatgatgc cgacgatgtt ggggcaggcg aagctgttgc cggttaagct gcggaagccg
      901 ccgttgagcc acgtggtatg gacgttgacg ccgggcgcgg tcagctcgat gacttcgccg
      961 tatttgaagc cgaagttgaa ggggttggtg tcctcgcttt tgctgacgga aatcagcgac
     1021 gaggaaaaga cggacgggaa gctcggctgc ggcaggttgt tggcggcggc gacgacgatg
     1081 cagcccgcct ggtaagcgcg gtcgagcagg tcgtggagcg gcgcgaaaaa ctgcggcttg
     1141 gttgttccga ggctcaggtt tatcaggcgg attttgcgtt tgatggcgta ttccagaccg
     1201 gcgagaaacg cctgtccgtc gcccgcgccg cccgcgccga gaaccttgat gctgtacaat
     1261 tcggcgtcgg gcgcaatcga agcgatgatg ccggcgcaag ccgtcccgtg tcctgccgaa
     1321 tcgcccgaat cggaaggcac gaacaaaacg cgcttgtgct cggagacggc ttcgaccgat
     1381 tcgacgactt tgccctgcaa ttgcgggtga ttggcgtcga tgccgctgtc gatgacggcg
     1441 acggcgacgc ctctgcccgt ccgatctttc cagctttctt cgggtccgaa cgggtttaaa
     1501 atgtcttgaa atttagtggt cacagttgaa gacgaaacaa tcgccataaa ttaataattt
     1561 aaaaaactgg tctccagact gttgtccgaa aattttaaga ccgcttccag aatttccttg
     1621 cagagacggc gctcggcttc gccgcgactg gcgatttcgc gcaccattat cgccagatca
     1681 atcatctcgc gatgctcgga ggtcgagcgc aggtttttca gccagtcgcg cacttccctg
     1741 aactcggttt ccttattgtc cgccagcatt ttcgcgccca aatcgcggaa cagacgcgcc
     1801 gattcgtaag cgttcaccag agaggcaatc gcttcggcga acagcgcggc tttgtccatc
     1861 tcaaacggcg tgaacgattc atagggcggc tcgcccagac ggttgacgta ttcgagaacg
     1921 ccgataatct cgccgttgta gcgcagcggc gtcgccagca tagtctgcgt gttgtagccg
     1981 gtttgtttat cgacctcggc gtaaaacgat gcttcctgcc cgacgtcggc aatcacgacg
     2041 ggctgtccgg aagagtaaac gaatccggcg acgcccttgc ccgaaggaat tttcattccc
     2101 tgcaactggt cggcgacttt gccgatggcg accagaaaac acaaatcgcc gtcgtcgccg
     2161 tcgcggatga tgacggacgc ttcctcggat ttcatcgaac cggcggaaag gtcgagcagg
     2221 tttctgatgg attgcgtcag cggctcggtc agcgcgttgg cgacgtcgac cgtttcgata
     2281 atcgtttgca gtttttcttc cagattcgga ttcgacatat gcgggttgcc ttatagagaa
     2341 ttcaagattc aggattcaaa attcaagatt caggattcaa gattcaaagt ttaagattca
     2401 agattcaaag tttaagattc aagactcagg attcgggatt ttacattatc cgacaaccaa
     2461 tcctgaatcc tgaattttga atcctgaatc ctaaatcctg aatcctgaat cctaaatccc
     2521 gaatccttat ctacactaaa gcaaacgccg aaaaaaaaca aaatttaact tggaaatttt
     2581 gccggattag acgtttcaca aggcatcaat aaattttatt ttcagtcggc agtcaaattc
     2641 gacttgcctt cggcggtcgt gtttgcgtaa gatgcgctta taacgtttcc gcgctgaaat
     2701 tttgagcttt caaacctatg aaaagaagag tctttgccgc tgtggttctg gcaatcgccg
     2761 gttttgtgtg ttccggcgtt tcggcgcagc agccgacgcg ccagccgatt acgcaattgc
     2821 ctaaacagga agtcgagccg tttaaaatcg ggcgcggcac atcgttttcg gcttcggttc
     2881 cgcgcgcgcc ggaaaataaa tctctcccca aaaatacaaa tccggtcgga accgaccaaa
     2941 tcaagcggga tttagccgag gcgctcgaag ttatcggcgc taattacgtt gacggaaaaa
     3001 ggattaatta caacgcgctg gcgaaatcct cgattgattc gatgctgcgc tcgctcgacc
     3061 cgcattcgaa tttttacgac gcggcacaat accgcgagat gctggaagag cagcgctcgg
     3121 aatacacggg catcggcgcg tcgattgtca attacacgat tgacggcgcg accgatacgt
     3181 ttatcacttc gacgtttccc gattcgcccg cctcgcgcgc cggattgcag ttcggcgata
     3241 aaatcgtcgc cgtcggcggc gttaatatga aaggcaaatc ttccttctac gtgcgcgagc
     3301 agattcgcgg cgagaaagga aaaatcgtcc gtctaacgat tgaaagagcg gcgacgctga
     3361 aaaccgaaac ggtggaaatc cgacgcagcg tcgtgccgca gccttcgatt cccgacgcct
     3421 atttattgcg acccggcatc ggctacgtgg atttatcgaa cggttttaat tacacgaccg
     3481 aagaagaact cgaaacggcg ctggaagatt tgcgcgagca gggtatgacg tcgctgattc
     3541 tggatttgcg cgacaatccg ggcggaattc tcgaacaagc cgtcaaggtc gcgcagaaat
     3601 ttctgcctgc gggaaatacg attgtcacgc agcgcgcgcg atttgccatc gaccaccgga
     3661 cgtggaaatc gacaaaccgc aatgcggaaa gctttccgct cgtcgttctg gtcaacggcg
     3721 gctcggcttc ggcttcggac atcgtcgccg gagcgctgca ggattacgac cgcgcattga
     3781 ttgtcggcga aaacactttc ggcaagggct tggtgcagag cattatcaat ctgccgctcg
     3841 gaaccggctt gaccctgacg acggcgaaat attacacgcc gtccgggcgc tcgattcagc
     3901 gcgattattc cagcggaaat ttatatgatt attttcgtca taaagcgagc tttgaagaac
     3961 gaaaaaaatc ggcgggcaaa acgattacgg gtcggacgat ttacggcgga aacggcatcg
     4021 agccggatga agtcgtgaag acttcgacgc cggtcgaagc gcagactgtt cttctcgacc
     4081 cgatgttctt tttcgcgcgc gaagccgtcg ccggcagatt tcgcgggctg gaaagctata
     4141 aatcagcccg tccggtgcaa tacggtcagc gcgtccgtcc gagcgatttt ccggtttccg
     4201 aggagctttt cgacgctttc aaaaaatttg tcgcgcagag caaatcgtgg cgcatttccg
     4261 ccgagcagct tgaagtcaac cggaaattta tcctgacgcg tttaagattt aatctggcga
     4321 cggcggcttt cggaaacgtt gcggcaaatc aggttttgat tgaagacgac ccgcaggtcg
     4381 ccaaagccgt cgagctgctg ccgcgagcac acagcctggc gcaggcggcg cgaaaaactt
     4441 tggagaaaag ataagccgca aagcgtttcc gctttgaaag tgataaacag ttactttgaa
     4501 cctggtaagc ggaaaacggt aagcggtaag ttgcagttat aaaataacta acttaccgct
     4561 taccgttttc cgcttaccag ttttttcgtt tacgggatga acgctgacgg aacgggttta
     4621 tccgtgccga cgccgaagtt gaccgtgtcg aaattctgcg acggcgagcc gaaagccctg
     4681 ccgatatacc acgaatttgt cgaaggacga aagacggcta aattggctac gccgtcgccg
     4741 tcataatccg ccggaacggg aatatcgccc gactgcccga attgaaccgg cgcaaatgct
     4801 ccgttgattg actgcaaaat ataccagacg ccgtccgacg gacgccagac ggcaatgtcg
     4861 gctttaccgt cgccgtcgta atccgacgct acgggtttgt cttcggcgac gccgaattga
     4921 gtcgccacaa attgattgct ggagctgttc agacgatacc agtatccgtt ggacggacgg
     4981 aacaccgcca cgtcagcttt tgcgtcgccg tcaaaatccg ctccggcaat cggcttatcg
     5041 ccgttctgac cgaaaggaac tgtgtcgaaa ttctgcgaag gcgttccggc tgagcgggcg
     5101 atataccacg ctcccgtaga aggtcgaaag acagccggat tagctctgcc gtcgccgtcg
     5161 taatcgcccg gcacgggaat gtcatcgccc gcgccgaact gtaaagcgcg gaaaccggca
     5221 ctcgattgca gcagatacca gacgccgtct gacgggcgat aaactgcaat atccgtgcgc
     5281 ccgtcgccgt cgtaatcggc tggaactatt ttgtcgattg agatgccgaa ctgctgtccc
     5341 gtaaatccgg cggtcgattg cagcagatac cacgttccgt cagccggtcg gtaaacggag
     5401 acgtccgctt tgccgtcgcc gtcaaaatca aagtttcggc gcggaaccgc gttgcgctcg
     5461 aatgcgccga tgtccgcgcg gcgcgcgccg tcgccgttgc cgtctgccgg acgcgaaaaa
     5521 ccgcgctggt caatcgacgg aaaattcgtc gggtcggcgg cgtcaatcgc cggactgccg
     5581 gcgtttaagg cgtgcgtcaa agtcgcgccg ccgttgccgg cgagcggagc catctgcggg
     5641 tcctgcccgt aaatattgcc ggtctggtcg ccgataatcg tcgccggcat aacttcgacc
     5701 agattgtagc cttgcgaatc gaatgtgcct ccgtagtccg gaaactcgtt ggcgagattg
     5761 tttgcaaata tcgagctgcg cgccttaagc gtagtggcgc aaacgccgcc gcaggtcgtc
     5821 gcaaaatttt cggcgaccgt tacatttgta agagtcccgg ttccggatga cataaggatt
     5881 ccgccgccca tcccgggcgc gccggatgcc tggttcaccg aattgccgct gatggtcgag
     5941 ttggtcaccg ttacactgct gccttgtaag ttagcaatgc cgccgccgcc ggaattactg
     6001 cccgcttgcg tgactttgtt gccgctgatg gtcgaattat tgatgacgag tgtggtcgga
     6061 ggagcaaaag tttgcgtcat acttatcgcg ccgcccgcca attgtgccgt gttgccgctg
     6121 atagtcgagt tattgattgt agtttgtccg ccgtgaagcg caagggcgcc gccgcgtgca
     6181 gccgagtttc ccgtcaagat gcagttattg atggt
//
LOCUS       DASWNE010000167        13713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_111113, whole genome shotgun sequence.
ACCESSION   DASWNE010000167 DASWNE010000000
VERSION     DASWNE010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13713
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111113"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VGB68_03480"
     CDS             <1..285
                     /locus_tag="VGB68_03480"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY0048322.1"
                     /translation="QCGYCTPGQICSAVALMQEAKNGEASYVTANLRTKAANLKLSDE
                     EIRERMSGNICRCGAYPNIVTAIREVHTGKPISAEAWNFSGAKEKEDETV"
     gene            272..1264
                     /locus_tag="VGB68_03485"
     CDS             272..1264
                     /locus_tag="VGB68_03485"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEY0048323.1"
                     /translation="MKPFKFSKASDAGNAVQAVSANEEAKFIAGGTNILDLMKEGVEN
                     PSELVDVSRLALTQIRANGNGVSIGALAKNTDTANHPLIRQNYPLLTQAILAGASQQI
                     RNMATNGGNLMQRTRCAYFYDVSMPCNKREPGTGCGALEGINRMHAIFGWSDKCVAVY
                     PSDMSIALAALDAVVKVRGTGGRERSIPFIDFHRLPGENPEKDNNLQHGELITSIELP
                     KSNFATKSYYLKVRDRASYAFALVSVAAALELNGNKIKQARVALGGVAHKPWRATEAE
                     KFLADKTATEENFKLAAEAEIRRAKLLEHNKFKVEMGRRAIVRALKRAVSGDRV"
     gene            1312..3441
                     /locus_tag="VGB68_03490"
     CDS             1312..3441
                     /locus_tag="VGB68_03490"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY0048324.1"
                     /translation="MSKRKNVVGESISRIDGILKVTGAAQYATDYAIKNPAHAFIVKS
                     TIAAGKILEIDAAEAEKSAGVLAVITHKNAPRLNPQSPIRGGGMLQSANVEFHGQHIG
                     VIVAETFEQARFAARLIKVRYEKADAKVDFAKHEKEAVKPKDKNLDDAARGDAEKAFQ
                     NAGHKIDVVYETPIEHHQPMEPHATIAVWESADKLTLYNGSQIVNGAQNAAAQTLNLK
                     PENVRIVTPHIGGGFGSKGGMWGNLILAAIAAKAVNRPVKLALTRQQMFNSVGLRQRN
                     RQHLRLAATKDGKLIALAHKTTTHSAINAEFLEPCGDCSKLMYDVPNSLISYRVVPMN
                     IILPTYTRGPGKSTGSFALESAIDELAHALEIDPIEFRIKNEPAKDLSNGKPWSSRKT
                     IECLKRGAEIFGWSKRKMQPRSVREGDDLIGYGVACGTYPARQRETSAIVKLTRSGSD
                     VSVGLELAASDLGTGTHTILAQTAADALGLPLEKIGVRIGDSTLPPAAGSVGSVGAAS
                     FANAVNDACVQAVTELQAKTNRQWFAAPTVAQLMEAANLSEFQTRVDAKPPAEAGNYS
                     AHSFNANFCEVRVNAGTGMVRIPRFVAVTGAGRILNLKTSRSQIIGGVIWGIGMALHE
                     ESVLDPRWGNFVTRSFADYHVPSNLDIGEIETIFVEEEDRIVNKLGVKGIGEVGIVGV
                     AAAVANAIFNATGKRVRELPITPDKLL"
     gene            3466..4983
                     /locus_tag="VGB68_03495"
     CDS             3466..4983
                     /locus_tag="VGB68_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY0048325.1"
                     /translation="MRKLANTIIFAAFIAFFVSVQAARACSCMANDTVDKDFVKVPNI
                     AVFKVQSVEKYSESEKGYGYEGIKQSKLTVEKVFKGNLKVGQVLTFAQGGGGDCIWTF
                     SEESIGQEYLFYLGSKPLDNKSSGRMVSSTTFENASPPQSVWAAFTCSRSGPVKYRTG
                     DVKYLENVSKVRGKTRLSGILSQSIRVAVEDEEAKNNLLSGSKVTVRGNGKNIELKTD
                     KDGFYEIYDLPPGKYKITPEKIYGYKALWGTTEDSVDIEIKDKSHTEQNFEFSIDNRI
                     RGRFFDAGGKPLKDVCLRLLPARGNPPQGFYEADCTDENGRFEIDEIPAGIYVIVVND
                     DNEISADEPFGKFYYPGALKREDAALIAVGAGDYRDDLIITAPQTAEVITVSGVLLYE
                     DDNPAADESVEFYAESEANQNDEEKSEDSRATTGRDGRFNIRILKGRKGKLLGSMLTY
                     EGEYEKCPKLDRLIKAQGGGVPDIKTPAISIEAGSDLTGVVLKFPFPSCKKAKTE"
     gene            5048..5566
                     /locus_tag="VGB68_03500"
     CDS             5048..5566
                     /locus_tag="VGB68_03500"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0048326.1"
                     /translation="MIGRTQLSVVEHDEELQAQTAQEKNADEAEIRRVVDEIDNACDA
                     KDWEKCRSFFADEVDVDFTSLAGGEPSRVKAGDLIGAWDANLFAEKKSFHMRSNHRVE
                     IKGSRAEVYSKGYAFNLLESGEVSGLWEVWGEYVHTLERGAGGWKVSGMTLNVAHKRG
                     DDRVREFQKEQK"
     gene            5613..7742
                     /locus_tag="VGB68_03505"
     CDS             5613..7742
                     /locus_tag="VGB68_03505"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY0048327.1"
                     /translation="MTKYIGKDINRVDGVAKVTGKAKYAAEFQLKNTAHGFLVLSTIA
                     KGNIKSINTSEAEKQAGVVRVFTHENMPKLAFKDSKDKDGVAPDGKPFRALQSDEIHF
                     NAQPIALVVAETFEQARYAASLVKAAYNAEKSETKLENVFDKAFYPDEKRKPKPRGNA
                     EDALKKSEFKIEAEYTIPVEHHNPIEPHATIAFWENDKLRVFDKTQWVYNVRNHLASV
                     FDIPETDVNVISPFVGGAFGGALRPAANVMLAAMAARELKRPVKIVYTRRQMFTAHGY
                     RPYTWQKISLGAEKSGKLTAIIHEAIGNTSPFESYEEGTNGATRTMYDCPNVHTDYKI
                     ARTNMATPLYMRAPGTVSGMFALESALDELAFKLKIDPLELRLINYAEKDPDNGKPFS
                     SKALRECYAEGAGVFGWKNRKLEPRSMREGDWLVGWGVATGTWGAFQAPASVRVTLKA
                     DGTALIESAASDMGPGTYTTITMIAAETLGVSMEKVKFELGNSDLPQAPVHGGSLTTA
                     SVGTAVQECCQKIMKKVAELGGNPEAVYFGEILRKSNTDKIVETHNSAPNFGERGKYS
                     IASHGAQFVEVKVDEDLGIIKVTRVVEATAAGKVINPKGAHSQELGGAVWGIGMALHE
                     ETEIDHRYGRIMNPTLAGYHVPVNADVHQVDTMFVEEDDPVVNALGVKGMGELGMVGI
                     PAAIANAIFHATGKRIRNLPITPDKLL"
     gene            7885..8994
                     /locus_tag="VGB68_03510"
     CDS             7885..8994
                     /locus_tag="VGB68_03510"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HEY0048328.1"
                     /translation="MKELQEILKKISALTPGEKAVLATVVDVKGSSYRRAGARMLIGA
                     GGETIGTVSGGCLESDVLERAKKVLRTGEPEVFIYDTTEAEDSVFSLNMGCRGVIRIL
                     LELFENENILARALRFASRHRQRQFIATMISSSRALAGGRIFYSNPEQFDFENLPADI
                     ENFEELKKDCLEFFVQNKSSEARAYRIGEDDFELFFENINPPLNLLLFGAGYDAIPLA
                     GFAGDLGWRVTLIDHRAAWASAERFPSADEIIVSRAENLDENLFKDEASVGVLMTHNY
                     EADREILYRLLNSSCRYIGALGPKKRTENLIAELRRAGKTFDESKLNRLYAPIGLDIG
                     AESPEEIALAIIAEIRSVLSNREAGFLRRRDGGIH"
     gene            9019..9675
                     /locus_tag="VGB68_03515"
     CDS             9019..9675
                     /locus_tag="VGB68_03515"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY0048329.1"
                     /translation="MNKKQENSKFTTRNPEPGTRNSKIGLILLAAGASSRLGNFPKQL
                     LEFQGETLIRQATKNALAAECGKICVVLGAEAEKIKREIGDLPVEIAVNEDWADGMSS
                     SLKCGLGKLLEIEPNLSAAVVMLGDQPLIDSTIINRLIEAFLKTQPSIVAGKYAETVG
                     VPAIFARSMFDELLHLADDGGAKQVIKKYAASVEKISVPEAAFDVDTQQDYENLLKLK
                     "
     gene            complement(9816..11102)
                     /gene="hemL"
                     /locus_tag="VGB68_03520"
     CDS             complement(9816..11102)
                     /gene="hemL"
                     /locus_tag="VGB68_03520"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEY0048330.1"
                     /translation="MNTSTSSKLFEEAQKHMPGGVNSPVRAFKSVGREPLYIKKAKGS
                     KMFDVDGNEFIDYIGSWGPMILGHAAKNVIDAIREAANDGTSFGASSEKEIELAVLIK
                     KFVPSVEIVRMVNSGTEATMSAMRLARGFTGREKIIKFEGCYHGHGDSFLSKAGSGVA
                     TLGLPDSPGVTKSTAKDTLNARYNDIKSVEDLVEQNKNEIAAVFIEPVAGNMGCVAAE
                     KEFLNSLRELCTAENILLVFDEVMSGFRLAKGGAQEIYAVKPDLTTFGKIIGGGLPVG
                     AYGGRGEIMQLIAPSGPVYQAGTLSGNPLAMTAGLETLKAIEADARFYPNLAASCEYL
                     YEGITNNLRELKLDYTLNRVGSMFTLFFTGEKVVDFDSAKTSDTKRFTAYFNRMLESG
                     VYLPPSQFEACFVSAAHSQEDFDATIKASYLALKNI"
     gene            complement(11250..12239)
                     /gene="hemB"
                     /locus_tag="VGB68_03525"
     CDS             complement(11250..12239)
                     /gene="hemB"
                     /locus_tag="VGB68_03525"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEY0048331.1"
                     /translation="MNRNQIYPNFPVSRLRRLRKTASLRDMFRETSLSKKDFIYPLFV
                     VEGENVKKEIGSMPENFQMSVDNILRECDELANLGIDSVLLFGIPDAKDEIGSGAYAA
                     NGIIQKAVRAVKKEFPEMLVITDVCLCEYTSHGHCGVIENEYVENDRTLELLAKEALS
                     HAEAGADIIAPSDMMDGRVAAIRNVLDQNGFAETPIMAYSAKFSSAFYGPFREAAQSA
                     PQFGDRKSYQMDSGNSDEALREIALDIQEGADIVMVKPALSYLDLIRRAKDNFNIPIA
                     AYNVSGEYSMIKAAAERGWLDGEKVMLEVLTSIKRAGADVIITYFAKEAAKLI"
     gene            complement(12251..13615)
                     /gene="hemG"
                     /locus_tag="VGB68_03530"
     CDS             complement(12251..13615)
                     /gene="hemG"
                     /locus_tag="VGB68_03530"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEY0048332.1"
                     /translation="MNKRVCIIGGGISGLCAAYRLKKQGVEVLLLEKNSFVGGNIQTE
                     KFDGFLIEYGPNSTLVSEHLFALADELGLSPEIAYADSTAKKRYILKNGELQALPLKI
                     VGLIGNKSFSAKAKLRLLKEPFIKSKAADDESISEFFARRLGREIVDYAVDPFISGIF
                     AGNPDNLSLKSAFPSLFEMEKNYGSLLKGAIFSPKEKKSKVPKTASRTVSFKNGMQTL
                     TDKLFAVLGESVRLNTEAFEIKKADNGKYSLQTNDAEINAEIFDSIVISTPSFVAARL
                     IKNFDAELSERLAGVYHPPIAVVFTAFKKENIKSDLDGFGFLIPKIERRKILGSLWNS
                     AIFAGRAPENFYLLTTFIGGARNAELAEKSEDVLMEIALEELNSILGLSGAPVFAKVK
                     KWEKAIPQYNIGYEKIIESIEDFKRQNAGIFYCSNFYRGISVSDCIKNSRLAAEEVLD
                     FLNN"
BASE COUNT         3511 a         3500 c         3670 g         3032 t
ORIGIN      
        1 cagtgcggct actgcacgcc cggacaaatc tgctcggcgg tcgctttgat gcaggaagcg
       61 aaaaacggcg aagccagcta cgtgaccgcc aacctgcgaa caaaagccgc gaatctgaaa
      121 ctgagcgacg aggaaatccg cgagcgaatg tcgggaaata tctgtcgctg cggcgcgtat
      181 ccgaacatcg tcacggcgat cagggaagtt cacactggta agccaatcag cgccgaagcc
      241 tggaatttca gcggcgcaaa ggagaaagaa gatgaaaccg tttaaatttt caaaagcgtc
      301 ggacgcagga aatgccgtgc aggcggtttc ggcaaacgaa gaggcgaaat ttatcgccgg
      361 cggcacgaat attctggatt tgatgaagga aggcgtggaa aacccttcgg aactggtcga
      421 cgtctcgcgc cttgcgctga cgcagattcg cgccaatgga aacggcgttt caatcggcgc
      481 gctggcgaaa aataccgaca cggcaaatca tccgctgatt cggcagaatt acccgctttt
      541 gacgcaggct atattagcgg gcgcatcgca gcagattcgc aatatggcga ccaacggcgg
      601 aaatctgatg cagcggacgc gctgcgcgta tttttacgat gtctcgatgc cgtgtaacaa
      661 gcgcgagccg ggcacgggct gcggcgcgct cgaaggcatc aaccggatgc acgcgatttt
      721 cggctggtcg gataaatgcg tcgccgtcta cccgtcggat atgtcaatcg cgctcgccgc
      781 gctggacgcc gtcgtcaaag tgcgcggaac cggcggcagg gaacgctcga ttccgttcat
      841 tgattttcat cgcctgcccg gcgaaaatcc cgaaaaagac aacaatttgc agcacggcga
      901 attgattacg tcaatcgagc tgccgaaatc gaattttgcg actaaatctt attatctgaa
      961 agtccgcgac cgcgcttctt atgcctttgc cctggtttcg gttgccgcgg cgctggaatt
     1021 gaacggcaat aaaatcaagc aggcgcgcgt agcgctcggc ggcgtggcgc ataaaccgtg
     1081 gcgcgcgacc gaagcggaaa agtttttagc cgacaaaacc gcgaccgaag aaaatttcaa
     1141 gctcgccgcc gaagccgaga tacgccgtgc caaactgctc gaacacaaca aattcaaagt
     1201 cgagatggga aggcgcgcga ttgtccgcgc gctgaaacgg gcggtgagcg gcgacagagt
     1261 ttaaagatta ttattttatt tcgcgggatt tgatttagga caaggattga aatgagcaag
     1321 cgaaaaaatg ttgtaggcga atcaatcagc cgcatcgacg gtattttaaa agtgaccggc
     1381 gcggcacaat acgcgaccga ttacgcgata aaaaatccgg cgcacgcttt tatcgtcaaa
     1441 agcacgattg ccgccggaaa aattctggaa atcgacgcgg cagaagcgga aaaatccgcg
     1501 ggcgttctcg ccgtcatcac gcacaaaaac gcgccgcggc tcaatccgca gagtccaatc
     1561 cgcggcggcg gaatgctgca aagcgcgaac gtcgagtttc acggtcagca catcggcgtt
     1621 atcgtcgccg aaactttcga gcaggcgcgt ttcgccgcgc gtttaatcaa agtcagatac
     1681 gaaaaagcgg acgcgaaagt cgatttcgca aagcacgaaa aagaagccgt caagccgaaa
     1741 gataaaaatc tggacgacgc ggcgcgcggc gacgcggaaa aagcttttca aaacgccggg
     1801 cataaaatcg acgtcgttta cgaaacgccg attgagcatc accagccgat ggagccgcac
     1861 gcgacgattg ccgtctggga aagcgcggat aaactgacgc tttataacgg ctcgcaaatc
     1921 gtcaacggcg cgcagaacgc ggcggcgcag accttgaatc tcaagccgga aaacgttcgc
     1981 atcgtcacgc cgcatatcgg cggcggtttc ggctcgaaag gcggaatgtg gggcaatctg
     2041 attctcgccg ccatcgccgc caaagccgtc aatcgccccg tgaagctcgc gctgacgcgc
     2101 cagcagatgt tcaattccgt cggcttgcgg cagagaaatc gccagcacct gcggctcgcg
     2161 gcgacgaaag acgggaagct gattgcgctc gcgcataaaa cgacgacgca ctcggcgata
     2221 aacgccgagt ttttagaacc gtgcggcgac tgctcgaagc taatgtatga cgtgccgaat
     2281 tcgctgattt cctatcgcgt cgtgccgatg aacattattt tgccgaccta cacgcgcgga
     2341 ccgggaaaat cgacgggcag cttcgcgctc gaatcggcga ttgacgagtt ggcgcacgcg
     2401 cttgaaatcg acccgatcga gtttcgcatc aaaaacgaac cggcgaaaga cctttccaac
     2461 ggaaagccct ggtcgtcgcg caaaacaatc gaatgtttga agcgcggcgc ggaaattttc
     2521 ggttggtcga agcgcaaaat gcagccgcgc tcggtgcgcg aaggcgatga tttaatcggt
     2581 tacggcgtcg cctgcggcac gtatccggcg cgccagcgcg agacttccgc gattgtaaag
     2641 ctgacgcgca gcggcagcga tgtgagcgtc ggtctcgaac tcgccgccag cgatttggga
     2701 accggaacgc acacgattct cgctcagacc gcggcggacg cgctcggtct gccgctcgaa
     2761 aaaatcggcg tccgtatagg cgattcgact ttgccgcccg ccgccggttc ggtcggctcg
     2821 gtcggcgcgg cgagctttgc caacgccgtc aacgacgcct gcgttcaggc ggtcactgaa
     2881 ttgcaggcaa aaacgaatcg tcaatggttt gccgcgccga cggtcgcgca attaatggaa
     2941 gccgccaatc tgagcgaatt tcaaacgcga gtcgacgcca agccgcccgc ggaagccggt
     3001 aattattcgg cgcacagctt taacgcgaat ttctgcgaag tccgtgtcaa cgccgggacg
     3061 ggaatggtca gaattccgcg tttcgtcgcc gtgacgggcg cgggacgaat tctgaatctg
     3121 aaaacgtcgc gctcgcaaat catcggcggc gttatctggg gaatcgggat ggcgctgcac
     3181 gaggaatcgg ttctcgaccc gcgctgggga aatttcgtga cgcgctcgtt tgccgattat
     3241 cacgttccgt cgaatctgga catcggcgaa atcgaaacga tttttgtcga ggaagaagat
     3301 cgtatagtca ataagctcgg cgtcaaaggc atcggcgaag tcggcatcgt cggcgtcgcc
     3361 gcggcggtcg ccaacgccat tttcaacgcg accggaaaac gcgtccgcga attgccgatt
     3421 acgcccgaca aactgcttta acgaaatatg agaaagctta taaatatgag aaagcttgca
     3481 aatacgataa ttttcgccgc gtttatcgct tttttcgtgt cggtgcaagc ggcgcgggct
     3541 tgctcgtgca tggcgaatga cacggtcgac aaagatttcg tcaaagtgcc gaacattgcc
     3601 gttttcaaag ttcaatcggt tgaaaaatat tcggaaagcg agaaaggtta cggttatgag
     3661 ggaatcaagc aatcgaagct gaccgtcgaa aaggttttca aaggaaatct gaaagtcgga
     3721 caggtattga ctttcgcgca gggcggcggc ggcgattgca tctggacttt tagcgaagaa
     3781 agcatcggtc aggaatatct tttttatctg ggcagcaaac cgcttgataa taaatcgtcc
     3841 gggcgaatgg tttcgtccac gactttcgag aacgcttcgc cgccgcagag cgtctgggcg
     3901 gctttcacct gttcgcgttc gggacccgtc aaataccgca ccggagatgt gaaatatctc
     3961 gaaaacgttt caaaagtgcg cggtaaaacg cggctttcgg gaattctgtc gcagagtatt
     4021 cgggtcgctg tcgaagacga agaagcaaaa aataatttac tttccggctc caaggttacc
     4081 gttagaggaa acggcaaaaa tatcgaattg aaaaccgata aagacgggtt ttacgaaatt
     4141 tacgatttgc cgcccggaaa atataaaatc acgcccgaga aaatctacgg ttataaagct
     4201 ttatggggca caacggaaga ttcggtcgat atcgaaatca aagacaaaag ccacaccgag
     4261 cagaacttcg agttttcgat tgacaaccgc attcgcggca ggttttttga cgcgggcgga
     4321 aaaccgctca aggatgtttg tctgcgcctg ctcccggcgc ggggaaatcc gccgcagggt
     4381 ttttatgaag ccgactgcac ggacgaaaac ggcagatttg aaatcgacga gattcccgcc
     4441 ggaatctacg tcatcgtcgt caatgacgac aatgaaattt cggctgacga accgttcggt
     4501 aaattttatt atccgggcgc tttaaaacgc gaagacgccg ccctcatcgc cgtcggcgcg
     4561 ggcgattacc gcgacgattt aatcattacc gcgccgcaaa ccgccgaagt catcacggtc
     4621 agcggagttt tgctgtatga agacgacaat ccggcggcgg acgaatcagt cgagttttac
     4681 gccgagagcg aggcgaacca aaacgacgaa gaaaaatccg aggattcgcg cgcgacgacc
     4741 ggcagggacg ggcgttttaa tatcaggatt ttgaaagggc gaaaaggaaa gcttctcggc
     4801 tcaatgctta cctatgaggg cgaatatgag aaatgtccga aattggacag gctgattaaa
     4861 gcgcagggcg gcggagttcc ggacattaaa accccggcaa tttccatcga agccggcagc
     4921 gatttaaccg gcgtcgtatt gaaatttcct tttccgagtt gtaaaaaggc gaaaactgaa
     4981 tagaattcag gaaaaaaagc aagatacatc gaaaaaaaat cagccgctcg ggcggcgctg
     5041 aaaaattatg attggacgaa cacagctctc ggtcgtcgag cacgacgaag aattgcaagc
     5101 gcagacggcg caggaaaaaa acgccgacga agcggaaatc cgccgcgtcg tcgacgaaat
     5161 cgacaacgcc tgtgacgcga aagactggga aaaatgccgc tcgtttttcg ccgacgaagt
     5221 cgacgtcgat ttcaccagcc tggcgggcgg cgagccgtcg cgcgtgaaag ccggcgattt
     5281 aatcggcgcg tgggacgcga accttttcgc cgaaaagaaa agctttcata tgcgctcgaa
     5341 tcaccgcgtc gagattaagg gcagccgcgc cgaagtttat tccaaaggct acgctttcaa
     5401 cctgctcgaa agcggcgaag tttcgggctt gtgggaagtc tggggcgaat acgttcacac
     5461 tctggagcgc ggcgcgggcg gctggaaagt ttcgggaatg actttaaacg tcgcgcacaa
     5521 gcgcggcgac gaccgcgtgc gcgaatttca aaaagagcag aaatgatttg gataaacggg
     5581 cggtttcggg aaaactattt aagagaaaag cgatgacgaa atacatcggg aaagacataa
     5641 accgcgtgga cggcgtggcg aaagtgaccg gcaaggcgaa atacgccgcc gagtttcaac
     5701 tgaaaaacac ggcgcacggc tttctcgttt taagcacgat tgccaaaggc aatatcaaat
     5761 caatcaatac gagcgaagcc gaaaaacagg cgggcgtcgt tcgcgttttc acgcacgaaa
     5821 atatgccgaa gctcgctttt aaagattcga aagacaagga cggcgtcgcg cctgacggaa
     5881 aaccttttcg cgctctgcaa tcggacgaaa ttcatttcaa cgcgcagccg atagctctcg
     5941 tcgtcgccga aactttcgag caggcgcgtt atgccgccag cctcgtcaaa gccgcttata
     6001 acgccgaaaa aagcgaaacg aaactggaaa acgtttttga taaagccttt tacccggacg
     6061 aaaaacgcaa gccgaaaccg cgcggcaacg ccgaagacgc tttaaaaaaa tccgaattta
     6121 aaatcgaagc cgaatacacg attcccgtcg agcatcacaa cccgatagag ccgcacgcga
     6181 cgattgcctt ctgggaaaac gacaagctcc gcgtttttga taaaacgcag tgggtttaca
     6241 acgtccgcaa tcatctggcg tccgttttcg acattcccga aacagatgtg aatgtgattt
     6301 cgccgtttgt cggcggcgct ttcggcggcg ctctgcgtcc ggcggcgaat gtgatgctgg
     6361 cggcgatggc ggcgcgcgaa ttgaagcgtc cggtcaaaat cgtctacacg cggcgtcaga
     6421 tgtttacggc gcacggctat cgcccttata cctggcaaaa aatatcgctc ggcgcggaaa
     6481 aaagcgggaa gctgacggcg attattcacg aagcgatagg aaacacatcg ccgtttgaaa
     6541 gttacgagga aggcacaaac ggcgcgacgc gcacgatgta tgattgcccg aacgtgcaca
     6601 ccgattacaa aatcgcgcgc acgaatatgg cgacgccgct ttatatgcgc gcgcccggca
     6661 cggtttcggg aatgttcgcg ctcgaatcgg cgctcgacga actcgccttt aaactgaaaa
     6721 tcgacccgct cgagctgcga cttatcaact acgccgaaaa agacccggac aacggtaagc
     6781 ctttttccag caaagccctg cgcgaatgct acgcggaagg agcgggcgtt ttcggctgga
     6841 aaaatcgcaa gctcgaaccg cgttcgatgc gcgaaggcga ctggctcgtc ggctggggcg
     6901 tggcgaccgg aacgtggggc gcgtttcagg ctccggcatc cgtccgcgtc actttgaaag
     6961 cggacggaac ggctttaatc gaaagcgcgg cttccgatat gggacccgga acttacacga
     7021 ccattacgat gattgccgcc gaaactttag gcgtgtcgat ggaaaaagta aagtttgagc
     7081 taggcaactc ggatttgccg caagcgccgg ttcacggcgg aagcttaacg accgcgagcg
     7141 tcggaacggc ggtgcaggaa tgctgtcaga agattatgaa aaaagtcgcc gagctcggcg
     7201 gcaatccgga ggctgtctat ttcggcgaaa ttctgcgcaa aagcaatacc gataaaatcg
     7261 tcgaaacgca caattccgcc ccgaatttcg gcgagcgcgg caaatattcg attgcttcgc
     7321 acggcgcgca gtttgtcgaa gtcaaggttg acgaagattt gggaattatc aaagtgacgc
     7381 gcgtggtcga ggcgaccgcc gccggaaaag ttatcaatcc gaaaggcgcg cacagccagg
     7441 aactgggcgg cgcggtctgg ggaatcggga tggctttgca cgaggaaacc gagattgacc
     7501 atcgctacgg gcgcattatg aacccgacgc tcgccggtta tcacgttccc gtcaacgccg
     7561 acgttcatca ggtcgatacg atgtttgtcg aggaagacga cccggtagtc aacgcgctcg
     7621 gcgtcaaggg aatgggcgaa ctgggaatgg tcggcattcc ggcggcgatt gccaacgcga
     7681 tttttcacgc gaccggaaaa cgcatcagaa atctgccgat tacgccggat aagctactgt
     7741 aaattttcgc cgtagagcgg agatttcaga aagaaaatta acagggacga aggagataaa
     7801 ccggggataa aagaaaaatt aattatccct tttgtcgcct tcgcccctgt taattctttt
     7861 tctgaaatct ctgcgataaa taatatgaaa gagcttcagg aaattctcaa aaaaatctcc
     7921 gctttgacgc ccggagaaaa agccgttttg gctactgtcg tcgatgtcaa aggctcaagc
     7981 taccggcgcg cgggcgcgag aatgctgatt ggcgcgggcg gcgaaaccat cggcacggtt
     8041 tcgggcggct gtctggaatc ggacgttctg gaacgagcga aaaaagtttt gcgaacgggc
     8101 gagccggaag tttttattta tgacacgacc gaggcggaag attcggtttt tagtttaaat
     8161 atgggctgtc gcggcgtaat tcgcattttg ctcgaattgt ttgaaaacga aaatattctc
     8221 gcgcgcgcgc ttcgattcgc ctcccggcac aggcagcggc agtttatcgc cacaatgatt
     8281 tcctcaagcc gggcactcgc cggcggaagg attttttaca gtaatccgga gcagttcgat
     8341 tttgaaaacc tgcccgctga catagaaaat tttgaagaac tgaaaaaaga ctgcctcgaa
     8401 ttcttcgtac aaaacaaatc ttccgaagcc cgcgcgtatc gaatcggaga agatgatttt
     8461 gaattgtttt ttgaaaacat taatccgccg ctgaatttac tgcttttcgg cgcgggttac
     8521 gacgcaattc ctttagcggg ttttgccggg gatttaggct ggcgcgtgac gctgattgac
     8581 caccgcgcgg cgtgggcgag tgcggaaaga tttccgtccg ccgacgaaat tatcgtttcg
     8641 cgcgccgaaa atttagacga aaatcttttt aaagacgaag cgagcgtcgg cgtcctgatg
     8701 actcacaact acgaagccga ccgcgagatt ttatatcgcc tgcttaattc ctcgtgccgc
     8761 tatatcggcg ctttgggtcc gaaaaagcgg acggaaaatt taatcgcgga gctgcgccgc
     8821 gccggaaaaa cttttgacga atcaaagctg aaccggcttt acgcgccgat cgggctggac
     8881 atcggcgccg aatcgcccga agaaatcgcg ctggcaatca tcgccgaaat ccgcagcgtt
     8941 ctgtcgaatc gcgaagccgg ttttctgcgc cggcgcgacg gcggaattca ttaaaggcga
     9001 aaggctaacg ggtaaagaat gaataaaaag caagaaaatt caaaatttac gacccggaac
     9061 ccggaacccg gaactcgaaa ctcaaaaatc ggtttgattt tgctggcggc aggcgcttcg
     9121 agccgtctgg gaaattttcc gaaacaactg ctcgaatttc aaggggaaac attgattcgt
     9181 caggcgacga aaaacgcgct ggctgcggaa tgcgggaaaa tttgcgtcgt cctgggcgcc
     9241 gaggcggaga aaatcaaaag ggaaatcggg gatttgcccg tcgaaattgc cgtcaacgaa
     9301 gattgggcgg acggaatgag ctcatcttta aagtgcgggc ttggaaaact gcttgaaatt
     9361 gagccgaatc tttccgccgc ggtcgttatg ctcggcgacc agccgttaat cgattcgacg
     9421 attatcaatc gtttaatcga agctttttta aaaacgcaac catcgattgt cgcgggcaaa
     9481 tacgcggaaa ccgtcggcgt gccggcaatt tttgcgcgtt cgatgtttga cgagctgctg
     9541 catctggcgg acgacggcgg cgcgaaacag gtaatcaaaa aatacgccgc ttccgtcgaa
     9601 aaaatctccg tgccggaagc ggcttttgac gtcgatacgc agcaggatta tgaaaatctg
     9661 ctgaagctta aataagataa gtcagattaa gaatttagcc gaaagaaaga gaagtaaaaa
     9721 gacaaaaggt aagaggcaaa acatagggaa ttgatttatt ttcgcttttt atttttgcct
     9781 tctgcccggc ttttctctct ttgcgggtaa acttcttaaa tattctttaa agccagataa
     9841 ctagccttga ttgtcgcgtc aaaatcctcc tgactatgcg cggcggagac gaaacacgct
     9901 tcgaactgcg atggcggaag ataaacgccg ctttcgagca tccggttgaa atacgcggta
     9961 aatcgtttag tatctgacgt tttcgccgaa tcgaaatcga cgactttttc ccctgtaaaa
    10021 aataaagtga acatcgagcc gacgcggtta agcgtgtaat cgagctttaa ttcccgtaga
    10081 ttattcgtga ttccttcgta taaatattcg cacgacgcgg caagattcgg ataaaagcgc
    10141 gcgtccgctt caatcgcctt taaagtttcc agaccggcgg tcatcgccag cggattgccc
    10201 gacaaagttc cggcttgata gacgggtccc gaaggcgcga tgagctgcat aatctcgcct
    10261 ctgccgccgt atgcgccgac cggcagaccg ccgccgatga tttttccgaa cgtcgtcaaa
    10321 tccggcttga ctgcgtaaat ttcctgcgcg ccgcctttgg cgaggcgaaa gccgctcatc
    10381 acctcgtcga aaacgagcag aatattttcc gccgtgcaaa gctcgcgcaa agaatttaaa
    10441 aattcttttt ccgccgcgac gcagcccata tttcccgcca ccggctcgat aaaaacggcg
    10501 gcgatttcgt ttttattctg ctcgaccaaa tcctcgaccg attttatgtc gttgtatctg
    10561 gcgttgagcg tgtctttcgc cgtcgatttc gtgacgccgg ggctgtccgg aagcccgagc
    10621 gtcgcgaccc cggaaccggc tttgcttaaa aacgaatcgc cgtgaccgtg ataacagcct
    10681 tcgaatttga tgattttttc gcgtcccgta aagccgcgcg ccagccgcat cgcgctcatc
    10741 gtcgcttccg tgccggaatt gaccattcgc acgatttcca ccgacggcac aaattttttg
    10801 atgagcacgg caagctcgat ttccttttcc gagctggcgc cgaagctggt gccgtcgttt
    10861 gccgcttccc ggatggcgtc gataacgttt ttcgccgcgt gtccgagaat catcggtccc
    10921 cacgagccga tgtagtcgat aaactcgttg ccgtccacgt cgaacatttt cgagcccttc
    10981 gcctttttga tataaagcgg ttcgcgcccg accgatttaa acgcgcggac gggcgaattg
    11041 acgccgcccg gcatatgttt ctgcgcttcc tcgaataatt tactgctcgt tgatgtgttc
    11101 attttttaat tcaatttcaa tcacggattg acacggataa ggacgggata aaaacaaata
    11161 cggataataa aaagatttat ctatatatct tatttatcct gttcttatct atcttgtgtt
    11221 tatctgtgcc aatccgtggt taataattcc taaatcagtt ttgcggcttc cttcgcaaaa
    11281 tatgtaatta tcacgtctgc tccggcgcgt tttatcgaag tcagaacttc gagcatcact
    11341 ttttcgccgt ccagccagcc gcgctcagcc gccgctttta tcatcgaata ttcgccgctg
    11401 acgttatatg cggcgattgg aatattaaaa ttatctttcg cgcggcgaat caaatccaga
    11461 taagaaagcg ccggtttgac catcacgatg tccgcgccct cctggatgtc gagcgcgatt
    11521 tcgcgcagcg cttcgtcgga gtttcccgaa tccatctggt aagacttgcg gtcgccgaac
    11581 tgcggcgcgg actgagccgc ctcgcgaaaa ggtccgtaaa aagcggacga aaattttgcc
    11641 gaataagcca taatcggcgt ttccgcgaaa ccgttctggt cgagaacgtt tcggattgcc
    11701 gcgacgcgac cgtccatcat atctgacggc gcgataatgt cggcgcccgc ttcggcgtgc
    11761 gaaagcgctt ctttcgccag cagttcgagc gtccggtcgt tctcgacgta ttcgttttca
    11821 atcacgccgc aatgcccgtg cgaagtgtat tcgcacagac aaacatcagt gataacaagc
    11881 atttcgggaa attctttttt gacggcgcgg acggcttttt gaatgattcc gttcgccgca
    11941 tacgcgcccg aaccgatttc gtctttcgcg tcgggaatgc cgaaaagcag aaccgaatcg
    12001 atgccgagat tcgccagttc gtcgcattcg cgcaaaatat tatcgaccga catctgaaaa
    12061 ttttccggca tcgagccgat ttcctttttt acattttcgc cttcgacgac gaacaaagga
    12121 tatataaaat cttttttaga gagagaagtt tcccgaaaca tatcgcgcag gctcgcggtt
    12181 tttcgcaagc gccgcaagcg cgagaccgga aaattcgggt aaatttgatt tctgttcatt
    12241 attgtaaaat ttaattgttc aaaaaatcta aaacttcttc ggcggctaga cggctgtttt
    12301 taatacaatc gctcaccgaa attccgcgat aaaaattgct gcaatagaaa attccggcgt
    12361 tttgtctttt gaaatcttcg attgattcga taattttctc ataaccgata ttgtattgcg
    12421 gaatcgcctt ttcccatttt ttcacctttg cgaaaacggg cgcgccgctc aagcccaaaa
    12481 tcgaatttaa ttcttcgagc gcgatttcca tcaaaacatc ttcactcttt tcagccagtt
    12541 cggcgttgcg cgcgccgccg ataaaggtcg tcaataaata aaaattttcc ggcgcgcgcc
    12601 ctgcaaaaat cgccgagttc caaagacttc cgaggatttt ccggcgctcg attttcggaa
    12661 tcaaaaagcc gaaaccgtcc aaatcagact ttatattttc ctttttaaaa gccgtgaaaa
    12721 cgacggcaat cggcgggtga taaacgcctg cgagccgctc ggacaattca gcgtcgaaat
    12781 tctttatcaa gcgcgcggcg acaaatgaag gcgtcgagat gacaatcgaa tcaaaaattt
    12841 cggcgtttat ttcggcgtcg tttgtttgca ggctgtattt tccgttatcc gcttttttta
    12901 tctcaaaagc ttcggtgttt aatctgacgc tttcgccgag aacggcaaaa agcttatccg
    12961 ttaaagtctg cattccgttt ttaaacgaaa cggttcgcga agcggttttc ggcactttcg
    13021 attttttttc cttcggcgaa aaaatcgcgc ctttcaaaag gctgccgtaa tttttttcca
    13081 tctcgaacag gctcggaaaa gcggacttga gactcagatt atcgggattt cccgcgaaga
    13141 ttcctgaaat aaacgggtcg acggcgtaat cgacaatctc gcgccccaat cgacgcgcga
    13201 aaaattcgga aatcgattcg tcgtccgccg ctttcgattt gataaaaggc tctttcaaaa
    13261 gccgcagttt tgctttggcg gaaaaacttt tgttgccgat taaaccgaca attttcaacg
    13321 gcagcgcctg cagctcgccg ttttttaaaa tatatctttt tttcgcggtc gaatcggcgt
    13381 aagcgatttc cgggcttaaa ccgagctcgt cggcaagcgc gaacaaatgc tcggaaacca
    13441 gcgtgctgtt cggtccgtac tcaatcagaa acccgtcgaa tttctcggtt tggatatttc
    13501 cgccgacgaa actgtttttt tcgagcagca gaacctcgac gccctgtttt tttaaacgat
    13561 aagccgcgca aagcccggaa attccgccgc cgatgataca aactcgtttg ttcatttaac
    13621 cgttatttcc ggctgaggcg gagttttccg tcacagaaat ttccaattta tcaaatacca
    13681 gctgcgccag cgcgtcgata aatttcggcg aat
//
LOCUS       DASWNE010000168         2557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_216426, whole genome shotgun sequence.
ACCESSION   DASWNE010000168 DASWNE010000000
VERSION     DASWNE010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216426"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..665)
                     /locus_tag="VGB68_03535"
     CDS             complement(57..665)
                     /locus_tag="VGB68_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048333.1"
                     /translation="MGQHVMCTARYLERSSYGKASLESDKLLFSGDFRLSISLREIKR
                     AVAENGELKITYNNETAAFHLEELAEKWAKKITSPKKLIDKLGVKAGASVAVLNISDQ
                     LFWEQLKERTENISPEALTSGIEFIFLQVENIGELEQIKNLRNFLAPNGALWVAIPKG
                     RKDIRVEDVIAAAKASDLVDVKLVGFSGTHSMYKLVIPLSSR"
     gene            complement(682..1344)
                     /locus_tag="VGB68_03540"
     CDS             complement(682..1344)
                     /locus_tag="VGB68_03540"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEY0048334.1"
                     /translation="MNVIGYGTFVRLAPVAGLLSRESFLNGEFFAARERQLWTRCLTT
                     KRRVEYIGGRLAGKIVANIYRLNRRQVPCGWSEIDIFPQADNRPVCRHFDGLEHAISI
                     SHSNGWAAALIVSESDSVAIDLENTAARKLPVSAMFSEAEIKQLESPQDAWRRWTVKE
                     AYCKLTGKGILGWEKELITYKMQNQLWLAIPFQMNFEGNRFLASGNCSSIMISIGFNQ
                     SR"
     gene            complement(1341..2399)
                     /locus_tag="VGB68_03545"
     CDS             complement(1341..2399)
                     /locus_tag="VGB68_03545"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEY0048335.1"
                     /translation="MNKSSPKISGAVKESYFGQQASPVVEGKKTVWQYELVISETEHK
                     LQNGAMYKVWAYNGQVPAPTIVAREGDWVRIRLINETSVNHTIHSHGLYVPQRMDGVP
                     HDHGLGQQNHNNPASSKDEFMPRPVAPGESFTYEYIARPAGTHWYHCHINTNEHLNRG
                     MAGALIVLPRQPEPKVDHDLIMLLQEWNSRYAQNGTPGSPKELNDYDFFTINGKSFPE
                     TKTVRVDLGETVRIRFINAGALPHSMHLHGHSFLVTHKDGFPLAEPAEMDTVLIGPGE
                     RVDIIILANNPGDWALHCHAAAHQTNNGIYPGGMMTHLKVGKISSPKEGEGPIGKDLK
                     SIRSVWRLSAHKYLGIGL"
BASE COUNT          574 a          651 c          619 g          713 t
ORIGIN      
        1 aactgctgat tttaaaagcc tcattgatta agcgcggcgc acttaatcgc tcttttttat
       61 ctggacgaca aaggtataac taatttatac atactgtgcg tcccggaaaa accgactaat
      121 tttacgtcta cgaggtccga agccttcgcc gcggcgatta cgtcttcgac gcgtatgtct
      181 tttcttccct tcgggatggc aacccagagc gcgccgttcg gagcgaggaa attccttaga
      241 tttttaattt gttcgagttc gccgatattt tcgacctgca aaaagataaa ctcgatgccg
      301 ctcgttaaag cttccggcga tatattttcg gttctctcct tcaactgctc ccagaacagc
      361 tggtcggaaa tattaagaac cgcgacgctc gcgcccgctt taactccgag cttatcgata
      421 agttttttgg gcgaagtaat tttttttgcc catttctccg ccagctcctc taaatgaaaa
      481 gcggcggttt cattgttata agttatttta agctcgccgt tttcggcgac ggctcgcttg
      541 atttccctga gcgaaatgct gagccggaaa tcgccgctga acagcagctt gtccgattcg
      601 agcgaagcct ttccgtaaga ggagcgctcc agataccgcg cggtacacat gacgtgttgt
      661 cccatatttt aaatcgtttt tctagcggct ttgattaaag ccgattgaaa tcattattga
      721 cgaacagttg ccggaagcca gaaacctgtt tccttcaaaa ttcatttgaa atggaattgc
      781 cagccataac tggttttgca ttttatatgt aatcagttct ttttcccagc cgagaatccc
      841 ttttccggtc agcttgcaat aagcttcttt aacagtccag cgccgccagg cgtcctgcgg
      901 gctttccagt tgtttgattt cggcttcgga aaacatagcc gatacgggaa gttttcgggc
      961 agccgtattt tcgaggtcga tggcgaccga atcggattcg gaaacgatta gcgccgccgc
     1021 ccagccgttc gagtggctaa tcgaaatcgc gtgttcgagc ccgtcaaaat gcctgcatac
     1081 ggggcgatta tcggcttgcg gaaaaatatc tatttcgctc cagccgcaag gcacctgccg
     1141 ccggtttaaa cgataaatat tggcgacgat tttaccggct agccgcccgc cgatatattc
     1201 gacacggcgt ttcgtcgtca aacatctcgt ccacaactgc cgctcgcggg cggcaaaaaa
     1261 ttctccgttt aaaaagcttt ctctggaaag cagtccggct acgggagcga gcctgacaaa
     1321 agttccgtaa ccgataacat tcatagcccg attcctaaat atttatgcgc cgagagtctc
     1381 cagaccgagc gtatagattt taaatctttt cctatcggac cttcgccttc tttcggcgag
     1441 ctgattttgc ccactttcag atgagtcatc attccgcccg gatatatacc gttattggtt
     1501 tgatgggcgg cggcgtgaca gtgcagcgcc caatctccgg gattgttagc cagaataatt
     1561 atgtctactc tttcgccggg accgattaat accgtgtcca tttcagccgg ttccgccaga
     1621 gggaaaccgt ccttgtgagt tactaaaaag ctgtgcccgt gcaggtgcat cgaatgcggc
     1681 agcgcgccgg cgttaataaa acgaatccga actgtttctc cgagatcgac ccggacggtt
     1741 ttcgtttccg gaaacgattt gccgttgatt gtgaaaaaat cgtaatcgtt taattctttc
     1801 gggctgcccg gcgttccgtt ctgcgcgtag cggctgttcc attcctgcaa gagcattatc
     1861 aggtcgtggt ctaccttcgg ttcgggctgg cgcggcagaa caatcagcgc acccgccatt
     1921 ccgcgattca gatgttcgtt tgtattgatg tggcaatgat accagtgagt tccggcggga
     1981 cgggcaatat attcataagt aaagctttcg ccgggcgcga ccgggcgcgg cataaactcg
     2041 tcttttgacg aagccggatt gttatgattt tgctgtccta aaccgtggtc gtgcggcacg
     2101 ccgtccatcc tttgcgggac gtataatccg tgggaatgaa tcgtgtgatt gacggacgtt
     2161 tcgttaatca ggcgaatgcg cacccaatct ccttcgcggg cgacaatggt cggagccgga
     2221 acctgaccgt tatacgccca gactttatac atcgcgccgt tttgcagttt gtgttcggtc
     2281 tcggaaatga ccagctcata ctgccagacg gttttttttc cttcgactac cggtgaagcc
     2341 tgctgcccga aatagctttc ctttactgcg cccgagattt tagggctgct cttattcaaa
     2401 ttacttttcg tcaatgtcga atccgtagaa ttttttacgg cgtcgtgagt gtgctgcccg
     2461 cgagcgactg tcgagcttag gcagagcgat aacccgatta atacggacaa acgcaatatt
     2521 cgatatttaa tgagcggaag tttcataatt ttatcca
//
LOCUS       DASWNE010000169         3857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_570331, whole genome shotgun sequence.
ACCESSION   DASWNE010000169 DASWNE010000000
VERSION     DASWNE010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570331"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VGB68_03550"
     CDS             <1..454
                     /locus_tag="VGB68_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048336.1"
                     /translation="GSVPFIILALGRPGGDFTGFLVLMGVGCALMYFYYSTVYSTIQD
                     VVEPGLRGTAMSLYFMAMYLLGASLGPYAIGLISDYFTQQAAQNAGVTIFTQQSLEPF
                     RAAGLHSAMYIIPVLSILLTLVMFAASRTVTKEIEKLQSWMRESFTAK"
     gene            complement(489..611)
                     /locus_tag="VGB68_03555"
     CDS             complement(489..611)
                     /locus_tag="VGB68_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048337.1"
                     /translation="MKLSDVINRFARLCGEKIPPSLSHLTGIIVFARARDFILL"
     gene            1529..2860
                     /locus_tag="VGB68_03560"
     CDS             1529..2860
                     /locus_tag="VGB68_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048338.1"
                     /translation="MTKNKRAKTSRKFIEIAAAVFLLLNLNSAAAAQTERRYPWQYFW
                     QQSLAAYKNRDYAAFLENSRKAVESGPPNHPTLFYNVARAYSLNGNKTEALRWLEKTL
                     RLGFGAEAVDGSDFLFLREPGEYSEIRKLLEKVKAPKVKSRTAATIFEPDLIPESVAF
                     DSSDKSFYVGSFYKRKIVRIDARGRTSDFIAEARDGLGSVVGIKIDAARKVLWVLNNI
                     APEMKNFDKTRAGFGMLHRYELKTGKLIKKYELSNQPRPHLLNDLVIARSGDVFITDS
                     LSSVVHVLRRDRDALENFVSLEPYSYPNGIALSKDENRLYVATLNGVSVIDPKTGKSA
                     PLAHAETIALAGIDGLYFYENSLLAIQNFESPNRVVRFHLNETGDRALRADVLESNHP
                     LYKLPTTGALDRRNFYYIANSQLRSFDESGKLPPPEKLQNVTILKLDLPKP"
     gene            2875..>3857
                     /locus_tag="VGB68_03565"
     CDS             2875..>3857
                     /locus_tag="VGB68_03565"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0048339.1"
                     /translation="MNKLTYILIIVFLFALAFRQDASAQEDKQSAPRKSMAEIVNAPV
                     VYKIAGMERAAIKKDLNYKTVETSPPLKMDVYSPPDLSKNERRPAVIFIHGGAGAQYN
                     PKNWGVYVSWGKLAAASGFVGITFTHRLAFPKTMVTEAAADLTDAINYVRANSDALGV
                     DKDRVCLASYSAGGPMLSLAMREKPEYVRCLVAFYSFLDIRQSAAHRENETPETLGKF
                     SPITYLDENAAKLPPLFIARAGRDEIPTMNDSIDRFVREALSKNAGVEVMNHPAGVHG
                     FDNQTDDERSREIIRRALEFMRENLEPPAKAEKSVDEAQELLTIEHKRSAA"
BASE COUNT          977 a          984 c          977 g          919 t
ORIGIN      
        1 aggctcggtg ccttttatca tactcgctct gggcagaccc ggcggcgatt ttacgggctt
       61 tttagttttg atgggcgttg gctgcgcgct gatgtatttc tattattcga cagtttattc
      121 gactattcag gatgtcgtcg agccgggttt gcgaggcacg gcgatgtcgc tctattttat
      181 ggcgatgtat ctgctgggcg cgtcgctcgg tccttacgcc atcggtttaa tcagcgatta
      241 tttcacgcag caggcggcgc aaaacgccgg cgtgacaatt ttcacacagc agtctctgga
      301 gccgtttcgc gccgccggac tgcattcggc gatgtatata attccggttt tgagcatttt
      361 gctgacgctc gtgatgtttg ccgcttcgcg cacggtcacg aaagaaatcg aaaaactgca
      421 aagctggatg cgcgaatcgt ttacggctaa atagaacgca ttttcgtact tcgataatga
      481 acgataattc aaagcaaaat aaaatcccgc gctcgcgcga aaacgattat tcccgtcaaa
      541 tggctgaggc tcggcggaat tttctcgccg cacagacggg caaagcgatt aatcacgtcg
      601 gacaatttca ttcgcccgat tttattcgaa ggcgtcgcgg catttgcgca aaacgcaagc
      661 cgatttttaa ccgcacgcgc agtgcctctg tgtaaaaaac agatgccgtt gatttgcgta
      721 attaaatttt ggcttgattt tctgaaacgg aatgacgtaa gatgcggtgt tggataggtc
      781 acatcttttg atacattttg tttcaaacag gtgaaccgag ttgcctgagc gataacgcca
      841 attatcgctc aggcttttct tttcgattca tccaaaatca attttctgcc aaagaatcaa
      901 aaagccgctg aacccattcg acaggctcaa cggctttttc taataaattg ttttccgaaa
      961 atgttattcc ccgtccgacc ggaaaagaat aacaaagaca acgcgccgcg aaagcgtggg
     1021 tcgttaatct actcttcaaa ctacaggaat tacccgtcat atcttattaa tcaaatcctg
     1081 gattttcggc ttttcgatta aacccggttt ttagtttacc ggcagacgaa aatctacttt
     1141 tatttgccgg aaatatctac tttaaatttt tatgtgctgg tttataagat agtgattgct
     1201 aaactagcat aggtcaaaat taagtgtcaa tatttttttc ggatttctta aaaataattt
     1261 tgaaaaggct taaatcgctc gacgcttccg ccgggcgggc tttacgcggc aaagcccgat
     1321 ttacggaaaa acactgattg gcgcggcgct cgtattcgaa attcgcgcag ttttctttgc
     1381 ggcagccggc gattacggat tcatgacgcc gaaaataaat cggcttgaat tcagaccccg
     1441 atattgtggt ttcgtcacga aatatcgccg ctcgaataaa agcgcttcct ttcccggtta
     1501 aataatctaa taattgcaac cgtgcattat gacgaaaaat aaacgggcga aaacgagccg
     1561 aaaatttatc gaaatcgcgg cagccgtatt tttactgctt aacctgaatt ccgccgcggc
     1621 ggcgcagacc gagcgccgtt acccgtggca gtatttctgg cagcagtcgc tggcggcgta
     1681 taaaaacagg gattacgccg cctttctcga aaattcgcgg aaagccgtcg agagcggtcc
     1741 gccgaatcac ccgacgcttt tttacaacgt cgcccgcgcc tacagcctga acggcaacaa
     1801 aacggaggcg ctccgatggc tggagaaaac cctgcggctc ggtttcggag ccgaagccgt
     1861 cgacggcagc gattttttgt ttctgcgcga gccgggcgaa tactcggaaa tcagaaagct
     1921 gctcgaaaaa gtcaaagcgc cgaaagttaa aagccggacg gcggcaacga ttttcgagcc
     1981 ggatttgata ccggaaagcg tcgctttcga ttcgagcgac aaaagttttt acgtcggcag
     2041 cttttacaag cgaaaaatcg tcagaataga cgcgcgcggc agaacgagcg atttcatcgc
     2101 cgaagctcgc gacgggctcg gctcggtcgt cggaatcaaa atcgatgccg cgcgaaaagt
     2161 tttgtgggtt ttaaataaca tcgcgccgga aatgaaaaac ttcgataaaa cgcgcgcggg
     2221 cttcggaatg ctgcaccgct acgagctgaa aaccggaaaa ttaatcaaaa aatatgagct
     2281 ttccaaccag ccgcgaccgc atctgctgaa tgatttggtc attgcccgca gcggcgacgt
     2341 ttttatcacc gacagcctgt cgagcgtcgt tcacgttctg cgccgcgacc gtgacgcgct
     2401 cgaaaatttc gtcagcctcg agccttacag ctacccgaac ggaatcgctc tgtcgaaaga
     2461 cgaaaacagg ctttacgtcg ccactctgaa cggcgtttcg gtcatcgacc cgaaaaccgg
     2521 aaaatccgcg ccgctcgcgc acgccgaaac aatcgccctg gcgggcatcg acggtttgta
     2581 tttttacgaa aacagccttt tggcgataca aaatttcgag tcgccgaacc gcgtcgtgcg
     2641 tttccatctg aacgaaacgg gcgaccgcgc gttgcgcgcc gacgttctcg aatcaaatca
     2701 tccgctttac aagcttccga cgaccggcgc gctcgaccga agaaattttt attatatcgc
     2761 caacagccag cttcgcagct ttgacgaaag cggaaagctt ccgccgccgg aaaagcttca
     2821 aaacgtgacg attctgaaat tggatttgcc gaaaccttaa acgcaggatt tattatgaac
     2881 aaactgactt acattttaat tatagttttt ttatttgctt tagccttccg gcaggacgcg
     2941 agcgcgcagg aggacaaaca gtccgcgccg cgcaaaagca tggcggaaat cgtcaacgcg
     3001 cccgtcgttt ataaaatcgc cggaatggag cgcgcggcaa tcaaaaagga tttgaattac
     3061 aaaacggtcg aaacgagccc gccgctgaaa atggacgttt attctccgcc ggatttgtca
     3121 aaaaacgagc ggcgtccggc ggttattttt attcacggcg gcgcgggcgc ccagtataat
     3181 ccgaaaaatt ggggcgtata tgtttcgtgg ggaaaactgg cggcggcttc cggtttcgtc
     3241 ggcataactt ttacgcatcg cctggcattt ccgaaaacga tggtcacgga agccgccgcc
     3301 gacctgaccg acgcgataaa ttacgtccgc gccaacagcg acgcgctcgg cgtcgataaa
     3361 gaccgcgttt gtctggcttc gtattcggcg ggcggtccga tgctgagcct cgcgatgcgc
     3421 gaaaagccgg aatacgttcg ctgtctggtc gctttttact cttttctgga cattcggcaa
     3481 tcggctgctc accgggaaaa tgaaacgccg gaaacgctcg gaaaattttc gccgattacc
     3541 tatctggacg aaaacgccgc gaagcttccg ccgctgttta tcgcccgcgc cggacgcgat
     3601 gaaatcccga cgatgaacga ttcaatcgac cgcttcgtcc gcgaggcttt gtcaaaaaac
     3661 gccggcgtcg aggtgatgaa tcatccggcg ggcgttcacg ggttcgacaa ccagacggat
     3721 gacgagcgct cgcgcgagat tatcaggcgc gcgctggaat ttatgcgcga gaacctggag
     3781 ccgcccgcca aagcggaaaa aagcgtcgat gaagcgcagg aattattaac tatcgagcac
     3841 aaacgcagcg cggcgat
//
LOCUS       DASWNE010000170         4338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_253008, whole genome shotgun sequence.
ACCESSION   DASWNE010000170 DASWNE010000000
VERSION     DASWNE010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253008"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VGB68_03570"
     CDS             <1..277
                     /locus_tag="VGB68_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048340.1"
                     /translation="ILNFENLSPQENRLQKNFLKRDKKNVSGSNSFTLATANASIGDS
                     MNSDIPGSPSSSKNVTSGMGSVLRQTIRRRFSQTITTRGWKNASSFN"
     gene            446..3409
                     /locus_tag="VGB68_03575"
     CDS             446..3409
                     /locus_tag="VGB68_03575"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY0048341.1"
                     /translation="MRFRKILSFFLLYIFTVNSVVAGLSVVKSSGISLTGADGIRYVG
                     TSGISLTGADGFLNYKSNGISLTGADGISLTGADGISLTGADGATYTGANGISLTGAD
                     GISLTGADGISLTGADGAISLTGADGTTYSADSIIIRGADGISLTGADGAISLTGADG
                     ISLTGADGISLTGADGISLTGADGISLTGADTITGFNSNGVVFTLVSPTGISLTGADG
                     AISLTGADGISLTGADGISLTGADHENPPVTSGLQSVDPELALWLNQATDDSNVNAVI
                     VFHQYPTPTDLAKLRQLGIVGGTIYRVLPMVMASATRQQLIAVSRLPQVRSIYGNRTL
                     SSNIDPFFNKTHVQSINADRDLQAHNSGVSVSGRGVTVAVLDTGVNSLHNDLSGKVVQ
                     NVRLQDLQSAAAGFVNPLAVENVPNTDLVSGHGTFVSGVIAGSGSMSGGKYSGVAPGA
                     RILGLGAGDLNLTHVLAGFDYILEKGAGYNVRVLNCSFSANTVYDFNDPVNIATKTLT
                     GRGVTVVFSAGNSGAGNGTLNPYAAAPWVVSVGATDDKGNLAGFSSRGVFGDAAHTPS
                     LVAPGVSVVSLRSTVSQTGVLGVPTDLQRLSATEIPFYTTASGTSFSAPLVAGTIAMM
                     LEANPNLNPAEIKDILQRSATPLPLKYRHEAGAGMLNAYAAVLEAAFPARRTGIFRAS
                     LETNSVKFITSVTQTFNQTVVPNLSTVTDLSIPANAIQSNISIAWGGQSVNDLSMKVY
                     DSNGVLRGESNNLNTTELTGRREKVNLINPPNQTFQAVIRHTTSNFASVQNVFGLVET
                     SRVEYAPFGDVAGLPLDAQSDIYNSLRTFLMLPQGKDFRPTTVVSRAQLAEAIVRGGR
                     VPQYVAKTPMYSDIGDLTTRSVVESVQSNPSGKLIFDVASGNAFLPDASATKLVAAVA
                     FVKAANLENLTATTPFPWAVADANLIPSQWRGYVAVALQKGFIKLDGNNFNPNRPINR
                     LELAQAINKIVRTGN"
     gene            3777..>4338
                     /locus_tag="VGB68_03580"
     CDS             3777..>4338
                     /locus_tag="VGB68_03580"
                     /inference="COORDINATES: protein motif:HMM:TIGR04214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSLREA domain-containing protein"
                     /protein_id="HEY0048342.1"
                     /translation="MSPAATFTVTTPIDGNDGTCDSNCSLREAISAANASPGDDVIVF
                     SSSFNDPRTIPLNGTELRIVNNGTLTINGRGAEFLTINANFSSRIIRNDGATVIINGV
                     TIAGGTGTGTVSGGLGGAVLNRLGGNLTFNGSKLWGNTATQGGAIYNDAGSLTLNNTT
                     FYQNMATTGGGAVYSTGALTLNNSNLY"
BASE COUNT         1023 a         1174 c         1169 g          972 t
ORIGIN      
        1 aattttaaat tttgaaaatc tctctcccca ggagaatcgc ctgcaaaaga attttttgaa
       61 gcgtgataaa aaaaatgttt ccggaagcaa ttctttcacc ctggcaaccg ccaatgcgag
      121 cattggcgat tcgatgaact cggacattcc ggggagtccg agttcatcga aaaacgttac
      181 gagcggtatg ggcagcgtgc ttcggcagac gattcggcga cggttttcac aaacgataac
      241 aactcgtggc tggaaaaatg catcaagttt caattaaggc tcggcgaacc ggtgcagaac
      301 tgaggcacgc atattgctga aactgaaaaa cgactggcat cgagctccca cctgatgcgg
      361 ttttcctaaa aaagcggttg aatttaatac tttttgaaat tacaattgta attctttaat
      421 acacaatctg gaatttacat agcctatgcg ctttagaaaa attctttctt tttttctgtt
      481 atacattttt accgtcaact ccgtcgtcgc cggacttagt gtcgtcaaat ccagcggcat
      541 cagcttaacc ggcgctgacg gaatacgcta tgtcggaact tccggcatct cgctgaccgg
      601 cgccgacgga tttctcaact ataaatcaaa cggaatttcg ctgaccggcg ctgacggcat
      661 tagtttaacc ggcgctgacg gcatcagctt gaccggcgct gacggcgcga cctacacggg
      721 cgcgaatgga atcagcttga ccggcgcgga cggcatttca ttgactggcg ccgacggcat
      781 cagtttgacc ggcgctgacg gcgcgatttc attgaccggc gctgacggca caacttacag
      841 cgccgattca attatcatca gaggcgccga cggaatttca ttgaccggcg cggacggcgc
      901 aatatcttta accggagcgg acggaatcag cttaacgggc gcagatggaa tcagcttgac
      961 cggcgcggac ggcatttcac tgaccggagc ggacggaatc agcttgaccg gtgctgatac
     1021 aattacgggc tttaattcca acggagttgt cttcaccctc gtttctccca caggaatttc
     1081 gttgaccggc gcggacggcg cgatttcatt gaccggagcg gacggcatca gcttaacggg
     1141 cgcggacggc atttcattga ccggcgccga ccacgaaaat ccgccggtta ccagcggctt
     1201 gcagagcgtc gacccggaac tggcgctatg gctgaatcag gcgacggacg acagcaacgt
     1261 caacgccgtt atcgtttttc atcaataccc gacgccgacg gatttggcga aattgcggca
     1321 gctcggcatc gtcggcggaa cgatttatcg cgtgctgccg atggttatgg cgtcggcgac
     1381 gcgccagcag cttattgccg tttcgcgtct gccgcaggtt cgctcgattt acggcaaccg
     1441 gactttatcg tcgaatatcg acccgttttt taataaaacg cacgtgcaga gcattaacgc
     1501 cgaccgcgat ttgcaggcgc ataattcggg agtatcggtt tccggacgcg gcgttacggt
     1561 cgccgttctg gatacgggcg tcaattcact gcacaatgat ttgtcgggca aggtcgtgca
     1621 gaacgtccgc cttcaggatt tacaaagcgc cgccgccggt ttcgtcaatc cgctggcggt
     1681 tgaaaatgtt ccgaatacgg acctggtcag cgggcacgga acttttgttt cgggcgtgat
     1741 tgccggaagc ggctcgatgt ccggcggaaa atacagcggc gttgcgccgg gcgcgagaat
     1801 tctcggtctg ggcgcgggcg atttaaatct gacgcacgtg ctggcgggct tcgattacat
     1861 tctggaaaaa ggcgccggtt ataacgtgcg cgtcctcaat tgcagctttt cggcaaatac
     1921 cgtctacgat tttaacgacc cggttaatat cgctacgaaa acgctgaccg ggcgcggcgt
     1981 gacggtcgtc ttttcggcgg gaaattccgg cgcgggcaac ggcactctga atccgtatgc
     2041 cgccgcgccg tgggtcgtca gcgtcggcgc gaccgacgat aaaggcaatc tggcgggatt
     2101 ttcttcgcgc ggcgttttcg gcgacgccgc gcatacgcct tcgctggtcg cgcccggcgt
     2161 gagcgtcgtc agcttgcgca gcacggtttc gcagaccgga gttttaggcg ttccgaccga
     2221 tttgcaaaga ttgtcggcga ccgaaatacc gttttacacg accgccagcg gcacttcgtt
     2281 ttccgcgccg ctcgtcgcgg gaacgattgc gatgatgctg gaagcgaatc cgaatctgaa
     2341 tccggcggaa atcaaagaca ttctccagcg ctcggcgacg ccgctgccgc tcaagtaccg
     2401 gcacgaagcc ggcgcgggaa tgctcaatgc ttacgcggcg gttctggaag cggcttttcc
     2461 cgcaagaaga acgggaattt ttcgcgcgag tctggaaacg aactcggtga aatttatcac
     2521 ttccgtgact cagactttca atcaaaccgt cgttcccaac ttaagcacgg tgactgacct
     2581 ttcgattccc gccaacgcca ttcaatcgaa tatcagcatc gcctggggcg ggcagagcgt
     2641 caacgatttg tcgatgaagg tttacgattc gaacggcgtt ctgcgcggcg aatcgaataa
     2701 tctgaacacg accgagctga ccggcagacg cgaaaaagta aatttaatta atccgccgaa
     2761 ccagaccttt caggcggtca ttcgtcatac gacgagcaat ttcgcttccg tgcagaacgt
     2821 tttcggtctg gtcgaaactt ccagagtcga atacgcgccg ttcggcgacg tcgccggctt
     2881 gccgctcgac gcgcaaagcg acatttacaa cagtctgcgc acatttttaa tgcttccgca
     2941 aggcaaagat ttccgcccga cgaccgtcgt ttcccgcgct cagctggcgg aggcgattgt
     3001 gcgcggcgga cgcgttccgc aatacgtggc aaaaacgcct atgtattccg acatcggcga
     3061 tttgacgacg cgcagcgtgg tcgaaagcgt ccaatcaaac ccgtcgggca aattaatttt
     3121 cgacgtcgcg agcggaaacg cgtttctgcc cgacgcttcg gcgacgaagc tggtcgcggc
     3181 ggtcgctttc gtcaaagcgg caaatctcga aaacttaacg gcgacgacgc ctttcccgtg
     3241 ggctgtcgcc gacgccaacc tgattccgag ccagtggcgc ggctacgtcg ccgtcgcttt
     3301 gcagaaaggt tttatcaaac tggacggcaa caacttcaac ccgaatcgac cgataaaccg
     3361 gctcgaactg gcgcaggcga taaacaaaat cgtccggacg ggaaattaat ttcggagata
     3421 ctaaaataac taaataagtt aaatcgctcg cccggtttgt aatcataacg ccattttcac
     3481 ggcgcttgcc ggaaactaaa tccctgttta taatgctcgc agttaatcaa actgcgagca
     3541 aatccataat tgccgccgaa aattaacgaa ggtttttgcc ggctttgtgc cgcttgcctt
     3601 tatcttactg atttcacaat cgtaagcagc cccactacaa gaacgctcta aaatcattcg
     3661 tgaaggaaaa tttcatgaaa aaattattaa ttaccgcagt tattattctg tctttcttta
     3721 ccttttcaga tttcttcggg accggctctc aggcacaaac ttcagagact gtcgagatga
     3781 gtccggcagc gacatttacc gtcacgacac caatcgacgg aaacgacggg acgtgcgatt
     3841 cgaattgcag tctgcgcgag gcgatttcgg cggcgaatgc ctcgcccggc gacgacgtta
     3901 ttgtgtttag ttccagcttt aacgacccgc gaacaatacc tctgaacgga accgaacttc
     3961 gcatcgtcaa caacggcact ttgaccatta acggcagagg cgcggagttt ctgaccatta
     4021 acgcaaattt ttcgagccgg attatccgga acgacggcgc gacggtaata atcaacggcg
     4081 tcacgattgc gggcggaacc ggaacgggaa ccgtcagcgg cggactgggc ggagcggtgc
     4141 tgaacagatt gggcggaaat ctgactttca acggctcaaa gttgtgggga aacaccgcga
     4201 cgcaaggcgg cgcaatctac aatgacgccg gaagcctgac cctgaacaac accacttttt
     4261 atcaaaacat ggcgacgacc ggcggcggcg cagtttacag tacgggagct ttaacgctta
     4321 ataactcgaa cctttaca
//
LOCUS       DASWNE010000171        10244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_181707, whole genome shotgun sequence.
ACCESSION   DASWNE010000171 DASWNE010000000
VERSION     DASWNE010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10244
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181707"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..3668
                     /locus_tag="VGB68_03585"
     CDS             144..3668
                     /locus_tag="VGB68_03585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEY0048343.1"
                     /translation="MPTKSLFFLCFLFGALFVSSIRAERLPVRAYTTADGLANDGVNK
                     IVRDSRGFLWFCTNEGLSRFDGFGFKNYTQNEGLPHRNVNDFLETKSGDFLLATTGGL
                     AVFNPNGKAYRWDFLKSNLEQNSVEPPPLFRTFLPPNPKNEKKSQTVLSLAESVDGRI
                     YTGNRTGLFRVEKTNDEWTFHKIESAEWNDETVFAALKFDAAGYLWLVTSTGIFRMSP
                     DGAVKNFHGDGGSSILADSGGRIWIGSSGTNAGLRLFTVSSDGRDALLTKIYRKADGL
                     PEDRPMSDIVQTSNGRIFILVSTYLCEFLPQARENEPKFRVVQNGAFQSVAEDAGGGL
                     WLGTVQEGAWKLALSGFVKYDERDGVPAVNISSLSTDAAGNVFFTDGKQSISRLAEGK
                     FETLQPFGLNGRSWGNSQLDFQDSNGEWWIPSLEGLLRYASLKKFGDLAKTPPKKIYT
                     MADGLLGDGIFNLFEDSRGDIWITSTSKSVTLQLWERKSGIVRRFSIADGLPTANGVV
                     AFGEDDGGNIWFGFFFGGVARYRNGKFEFFTAEDGMAESSVNSILTDKAGRLWIATAS
                     RGVFRADNPAADKPIFTNLSTAEGLSSNQANCIAEDNFGHIYIGTGRGINRVDPVNGN
                     LKSFTQADGLPGSFVQYCRRDAGGAMWFVSRNSLVKFIPQSETALKPPPVFIGGINVG
                     GIAQPISELGAETVENLELNADQHQIRIDFFALGFNAGDRLRYQYKLNDEDWREPEDS
                     KSLNFNLAAGDYKFFVRAVNADNAASEVAATVSFKIAFPFWRRWWFLLSAAFFIGGTV
                     LAVERYRAARLRELENAFGKLSVSENRFRQMNEQSPLGIIVFAPDGSIRSVNRAYEDF
                     WGISFEQIKDWDFLSDEQNIKTGVAGKLRPVFLGETMTLPPISYVHRRNGAGVKIDEK
                     ADARWFESFAYPVKNGAGELLEVILVMEDVTDTKRAGEMEQKAKTDRLRELEQVRRRI
                     AADLHDDIGSSLTQISIFSEVLQQRVDKTNERVLEPLEFIASSSRELVDAMSDIVWAI
                     NPQKDFLSELNGKMHRFAADVFTAREIEFTYTASPTENIALGANLRREVFLIFKESIN
                     NIVKHAACRTVEIKLDIKNSEIYLSLRDDGAGFETDQINSGHGLVSMKRRAAGLNGKL
                     DIASSKTIGTTITLVVPFGAAGASDEKI"
     gene            3759..4430
                     /locus_tag="VGB68_03590"
     CDS             3759..4430
                     /locus_tag="VGB68_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0048344.1"
                     /translation="MNDTETAIAVAKIINVGIIEDRREIRDGLAMLINGTDGFACTGK
                     FGSMEEGLRRLGAELPDVVLCDIGLPGMNGIEGIKILKEKFSDLLVIMLTIYDDDERI
                     FDALCAGACGYLLKKTPPLKILESLKDAVTGGSPMSPEVARRVITIFRDIRPPERADY
                     NLTPHETRLLQMLVEGHNYKTAAVEIGVTVHAVSFHMRNIYEKLQVHSKSEAVSVALR
                     DRLVK"
     gene            4420..4791
                     /locus_tag="VGB68_03595"
     CDS             4420..4791
                     /locus_tag="VGB68_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048345.1"
                     /translation="MLNNSLYQTYSEYSLFDSQERVVHRPTRYLERLRRSKRPVESQR
                     QIANVIKLHGEWLEKSPFFQGLRIDEALSLVAGDDILEWINDQREAVISESTIHNREV
                     LLREMYKWLTTEEASVRRQTP"
     gene            complement(5006..6118)
                     /locus_tag="VGB68_03600"
     CDS             complement(5006..6118)
                     /locus_tag="VGB68_03600"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY0048346.1"
                     /translation="MVEKYDVVILGGGPAALTTAISIRLRSNLSVLVTDSGLPVCERV
                     GESAPSDLLHPLRHLKLHEVFLMDGHAACPGSAIVWGREHIGYNDNILNPMGPSWRLN
                     RQSFDNMLIRGAEERGVTIWWQTRYTGISKRGVPTADHHLVFSDINSKRSFEVQASWV
                     VDATGPAARFARDQGVKKQIDDRLFALACFSNINAGDMTMQTFLEAEAEGWWYAARLP
                     TDRVVCMYVTEAATLRQVKANNFEGWSAALNKTRLVGPILARLDLGNSVYHSFPIYSS
                     QLEQVAGHNWIAVGDAASCYDPVAAQGLYKALTDGVKAGHTIADALNGQPGTLTAYAE
                     GVKARYFQYYTNRNYLYGQEQRWKDSTFWSERCKSR"
     gene            complement(6111..8147)
                     /locus_tag="VGB68_03605"
     CDS             complement(6111..8147)
                     /locus_tag="VGB68_03605"
                     /inference="COORDINATES: protein motif:HMM:NF036537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LodA/GoxA family CTQ-dependent oxidase"
                     /protein_id="HEY0048347.1"
                     /translation="MSNNISKKTIFRVHPSINFARVGTSNEYYLSPETSAGMPLQGTE
                     TTGGLPIKPGTESDFITSADLRDKEGLLKRQAARFRLFETVLEGDDVYPSKNTVNEIE
                     IGSHLGDGRVVKDIIWTAHLANKKAAAYVVVNSQGVNAYNDKVPQLRNQDVYGDVNSA
                     NRLLKLVIDPGPRAISAAEKTCAKFDKTGSAAFGSLSGEIIPLPDYPINFPGGKLFEP
                     NGKLDSLGDILTDEKGRLLVLPGFGRAAAQYDEYGDPIPLTGDLNNAGWFDDSSDGPV
                     SATLLFTDGTAEAAFGAWVVCGDPAFAPQIRNVVSVWDDVFDVWIRDKELALQPEIYD
                     SETGNFNSAYQPAFETMIRPVFRAANLQRWTTLLPKIALRAHDAVDAIKSEDVPNETI
                     MAGLNFIRNPNVAPELNIGVPLMPLSVGDAGTSFLTVTKTQYFFLEQWSKNNFIRGDG
                     AALNQTELLDMASLSNCLGGRYVPGIEVSYVVRCTEIYMHDWRSSGAGPFRIMHKNLP
                     YAEASKDKPFLTGGWIPMHNMTDGLEPGDISKFMSVPWQTDYNSCSIHQPSINTLGVN
                     HTNGNETTLYWSWPAQRPDSVYVADEVVNNVLPNQKWAIRGPGAYALDPRSASTFQKA
                     LQSVTDWDRLGIVIQGTAISEGYSPEYYLEVQNKFDDAANADNPVLEWPFNTHG"
     gene            complement(8174..9145)
                     /locus_tag="VGB68_03610"
     CDS             complement(8174..9145)
                     /locus_tag="VGB68_03610"
                     /inference="COORDINATES: protein motif:HMM:NF024306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY0048348.1"
                     /translation="METIKNGLQIQNPEWTLDLLQKHIQYAVDLEFWTIPFYMSAMYS
                     VKDRSSDAYQFIQTAVNQEMLHLQSASNIANAYGFSPTFNVPVYVGKNVPHLNFDLDK
                     PDPRPEYTPFSAEIGPLDLERINAMCLIEYPDWDSSDKPDLNPDVTDYGNIGEFYLAL
                     RDGADQLKDKLHGGVKQVDLFSNFYRNMPAMTITESGRRGFYQVDLLIKLITDQGEGI
                     SKAEEDVQEVLQNTADDKRQELSHFEKFIRIKTAAPQATYPVKAISEYTDEDRRLLEI
                     LQNHFSGLCKNLEALFNGENPEDFFRMMLLVGADIQNCWKHEITPVF"
     gene            9971..10244
                     /locus_tag="VGB68_03615"
                     /pseudo
     CDS             9971..10244
                     /locus_tag="VGB68_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011071025.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2603 a         2478 c         2509 g         2654 t
ORIGIN      
        1 ccgctcgcgg tattcgccgg caaatcgtca cgctgatgga agaacgcgat agcattagtt
       61 ttacggctga tgaatcgaag ttatctttta accgaatcag tgatatgttt agggcgtaat
      121 tcataatcgc tgccgtttta ccgatgccaa caaaaagttt attttttctc tgctttttat
      181 tcggcgcgct gtttgtttcg agcatccgcg ccgagcgtct gccggttcgc gcctacacga
      241 ccgccgacgg tttggcaaac gacggcgtga acaaaatcgt gcgcgattcg cgcgggtttc
      301 tctggttctg cacgaacgaa ggattgtccc gtttcgacgg cttcggattt aagaattaca
      361 cgcaaaatga aggtttgccg caccgcaacg tcaacgattt tctggaaact aaaagcggcg
      421 attttctgct ggcgacaacc ggcggattag cggttttcaa cccgaacggc aaggcttatc
      481 gctgggattt cctgaaatct aatctggagc aaaactctgt tgagccgccg cctttatttc
      541 gcactttcct gccgccgaat ccgaagaacg aaaagaaatc gcagaccgtt ttaagtttag
      601 ccgaatccgt tgacggaaga atttacaccg gaaaccgcac cggactgttc cgcgttgaaa
      661 aaacgaatga tgaatggact tttcataaaa tcgaatccgc cgagtggaac gacgaaaccg
      721 tatttgcggc tttgaaattc gatgccgccg gttatttatg gctggttacc agtacgggaa
      781 ttttccgaat gtcgccggac ggcgcggtta aaaattttca cggcgacggc gggtcttcga
      841 ttttagccga cagcggcgga cgaatttgga ttggaagcag cggcacgaat gcgggtttgc
      901 gcctttttac cgtttcgtca gacggacgcg acgcgcttct gacgaaaatt taccgaaagg
      961 cggacggttt acctgaagac cgtccgatgt ccgacatcgt gcaaacctct aacgggcgaa
     1021 ttttcattct tgtctccaca tatttatgcg aatttttgcc gcaggcgcgt gaaaatgagc
     1081 cgaaatttcg cgtcgtccag aacggcgcgt ttcaatctgt agcggaagat gcaggcggcg
     1141 gtttgtggct cggtacggtt caggaaggag cgtggaaact tgccttgagc ggtttcgtca
     1201 aatacgacga gcgcgacggc gtgccggctg taaacatcag ctctttatcg accgatgcgg
     1261 cgggaaacgt cttttttacc gacgggaaac aaagtatttc acggcttgcg gaaggcaagt
     1321 ttgaaactct ccagccgttc ggcttgaatg gtcgcagttg gggaaacagc cagctcgact
     1381 ttcaagactc gaacggcgaa tggtggattc cgtcgctcga agggttgctg cgttacgcga
     1441 gcttaaaaaa attcggcgac ctggcaaaaa cgccgccgaa aaaaatctat accatggctg
     1501 acggattgct cggcgacgga attttcaatc tcttcgaaga ttcgcgcggg gatatatgga
     1561 taaccagcac cagtaaatca gttacgctgc agttgtggga gagaaaatcc ggcatcgttc
     1621 gccgtttctc gattgctgac ggtttgccga cagcgaacgg cgtggtggca ttcggcgagg
     1681 acgacggcgg caatatttgg ttcggctttt tcttcggcgg tgtggcgcgt tatcgtaacg
     1741 gcaaattcga gtttttcacc gccgaagacg gtatggcgga aagctccgtc aactcgatat
     1801 taaccgacaa agcgggtcgt ttatggatcg cgacggcgag ccgcggagtt tttcgcgctg
     1861 ataatccggc ggcggataag ccgattttta cgaatctttc aaccgccgaa ggtttgtcca
     1921 gcaatcaagc taactgcatc gcggaagaca atttcggaca tatttatatc ggcacgggac
     1981 gcggcatcaa tcgtgttgac ccggtaaacg gaaatctcaa atcgttcaca caggcggatg
     2041 gtttgcccgg cagcttcgtg cagtattgtc gccgggatgc aggcggcgcg atgtggttcg
     2101 tttcgcgcaa cagcctggta aaatttattc cgcagtctga aaccgcgctt aaaccgccgc
     2161 cggtttttat cggcggaatt aacgtcggcg gcatcgcaca accgatttcc gaactcggcg
     2221 cggaaaccgt cgaaaatctc gaattgaacg ccgaccagca tcagattcgg attgattttt
     2281 tcgctctcgg ctttaacgcc ggcgaccgtc tgcgttatca atacaaactc aacgacgaag
     2341 attggcgcga gccggaagac tcgaaatctt tgaattttaa tttagcggcg ggcgattaca
     2401 aatttttcgt gcgagccgtc aacgccgaca atgccgcaag cgaggtggcg gcaactgttt
     2461 cgtttaaaat tgcctttccg ttctggcggc gatggtggtt tttgctgtct gccgcgtttt
     2521 ttatcggcgg cacggttctg gcagtcgaac gctaccgcgc ggcgcgcctg cgcgaactcg
     2581 aaaacgcttt cggcaagctc tcggtttcgg aaaatcgctt tcgccagatg aacgaacaat
     2641 cgccgctcgg gattatcgtt ttcgccccgg acggctcgat tcgttcggtc aatcgcgctt
     2701 atgaggattt ctggggaatt agtttcgagc aaatcaaaga ttgggatttt ttgtcggacg
     2761 agcagaatat taaaacgggc gtcgccggga aactgcgccc tgtttttctc ggcgaaacaa
     2821 tgactctgcc gccgatttcc tatgttcacc gaaggaacgg cgcgggcgtc aagattgacg
     2881 aaaaagccga cgcgcgctgg tttgagtcgt tcgcctatcc ggtcaaaaac ggcgcgggcg
     2941 agctgctcga agtcattctg gtcatggaag acgtcaccga tacgaaacgc gccggcgaaa
     3001 tggaacagaa agctaaaacc gaccgcctgc gcgaactcga acaggtgcgc cgccgcatcg
     3061 ccgccgacct tcacgacgac atcggctcgt cactcacgca aatctcgatt ttcagcgaag
     3121 ttttgcagca gcgcgtcgat aaaacaaacg agcgggtttt ggagccgctc gaatttatcg
     3181 ccagctcgtc gcgcgaactg gtggacgcga tgagcgacat tgtttgggca atcaatccgc
     3241 agaaagattt tttgagcgag ctgaacggga aaatgcaccg cttcgccgcc gacgttttca
     3301 cggcgcgcga gattgagttt acctacacgg catcgccgac cgaaaacatc gcgctcggcg
     3361 cgaacttaag gcgcgaagtg tttttgattt tcaaggaatc aatcaacaat atagttaaac
     3421 acgccgcttg ccgaacggtc gaaatcaaac tggatattaa aaactctgaa atttatttga
     3481 gtttgcgcga cgacggcgcg ggctttgaaa cagaccaaat caacagcgga cacggtctgg
     3541 tcagtatgaa gcggcgcgcc gccggtttga acggcaaact cgatattgcg tcgagcaaaa
     3601 ctataggaac gaccatcacg ctcgtcgttc cgttcggcgc agccggcgcg agcgatgaaa
     3661 aaatctgaaa aatcgtttct gctttcagct acccgtataa gtagtgcatt tgcccgacta
     3721 ttaatttatc ttctctattt agaagcgaat aaaaaaatat gaacgatact gaaacggcga
     3781 ttgccgtcgc caaaataatc aacgtcggca ttatcgaaga ccggcgcgaa atccgcgacg
     3841 gtttagcgat gctgattaac ggcacggacg gtttcgcctg cacgggaaaa ttcggctcga
     3901 tggaagaagg cttgcggcgt ctgggcgcgg aattgcccga cgtcgtatta tgcgacatcg
     3961 ggcttcccgg aatgaacggc atcgaaggca ttaaaatcct gaaggaaaaa ttttccgacc
     4021 ttctcgtgat tatgctgacg atttacgacg acgacgagcg gattttcgac gcgctctgcg
     4081 ccggcgcgtg cggttatctc ttaaaaaaga cgccgcccct caaaatcctc gaaagtctaa
     4141 aagacgccgt gaccggcggg tcgccgatgt cgccggaagt cgcgcgccgc gtcatcacga
     4201 ttttccgcga cattcgcccg cccgaacgcg cggactacaa tttgacgccg cacgaaacac
     4261 gccttttgca aatgctcgtc gaaggtcata actataaaac cgctgccgtc gagataggcg
     4321 tgacggttca cgccgtttcg tttcatatgc ggaacattta cgaaaaactg caggttcact
     4381 ccaaatccga agccgtctcg gttgctctgc gcgaccggct tgttaaataa ttcactttac
     4441 caaacttatt cagaatattc tttgttcgat tctcaagaaa gagtggttca ccgtccgacg
     4501 cgctatttag agcggctgcg acgcagtaaa cgtcccgttg aatcacaaag acaaattgcc
     4561 aatgttatca agctgcatgg cgaatggctt gaaaaatctc ccttttttca gggactgcgt
     4621 atagatgagg ctttatcgct agtcgccggc gacgatattc tcgaatggat taatgatcag
     4681 cgagaggcgg taatatcaga aagtaccatt cacaacagag aggtgctgct cagggagatg
     4741 tacaagtggc tgactaccga agaagcctct gtccgccggc agactcctta gttcaatcat
     4801 attatagtgt ctcgaaaagg ggcgtttttg aatcattaac aaagtcgagg ttgtaagaat
     4861 ttttgtgtaa tcttcttatt ttattggagt ttaaaaataa tctggacaat gaaaataaga
     4921 ggagtagagt gagtttttta tcctgaaaaa cttgaccata tcacctttga aattcagttt
     4981 tgcaattttc atttcacgca cttttttaac gcgatttgca tcgctcactc caaaacgtag
     5041 aatctttcca gcgttgctct tgcccatata ggtaatttcg gttggtataa tattgaaaat
     5101 agcgagcttt tacgccttcg gcataagcgg taagtgtgcc gggttgaccg ttcaatgcgt
     5161 cggcaatggt gtggccggcc ttgacaccgt ctgttaatgc tttgtacagg ccttgggcgg
     5221 caacagggtc ataacaggaa gcggcatcgc cgacggcgat ccaattatga cccgctacct
     5281 gctctaactg tgatgaataa attggaaaac tatgataaac ggaattgccc agatcaaggc
     5341 gggcaaggat aggtccaacc agtcgagttt tgttcaatgc ggcgctccag ccctcaaaat
     5401 tatttgcttt aacttgccgc agcgtggcgg cttccgtaac atacatacaa accactcgat
     5461 cggtgggtag acgggcggca taccaccagc cttccgcttc cgcttccaga aaagtctgca
     5521 ttgtcatatc accggcattg atattactga aacatgccag ggcgaaaagc cgatcatcaa
     5581 tttgcttttt taccccctga tctcttgcaa aacgcgccgc cggtccggtc gcatccacca
     5641 cccatgacgc ttgcacctca aaactgcgtt tactgtttat atcggaaaaa acaaggtgat
     5701 ggtcagccgt cggaacgccg cgcttgctga tgcccgtata gcgtgtttgc caccaaatag
     5761 taacgccccg ctcttcggcg cccctgataa gcatattatc gaacgactgc cggttcagcc
     5821 gccatgaagg tcccattggg ttaaggatat tatcattgta gcctatgtgc tcacgtcccc
     5881 aaactatcgc actgcccgga caagcggcat gcccatccat tagaaaaact tcatgtaact
     5941 tcagatgcct gagcggatgc agcaaatcgg acggagcgct ttccccaacc cgttcacaaa
     6001 ccggaagtcc ggaatctgtc accaaaacgg aaagattgct acgtaatcga atcgagatcg
     6061 cagtggtcag ggcggcaggt cccccgccca gtattacgac atcgtatttt tcaaccatgc
     6121 gtattaaacg gccattccaa taccggattg tcggcattgg cggcatcgtc gaatttattt
     6181 tggacttcca ggtaatattc cggtgaatat ccttcactga tcgctgtccc ctgaattaca
     6241 ataccgaggc gatcccaatc ggtaaccgat tgcagagctt tttgaaaagt gctcgccgat
     6301 cggggatcca aagcatacgc tccgggtccg cgaatggccc atttctgatt cggcagcaca
     6361 ttattgacta cttcgtcggc gacatagacc gaatccggac gctgcgcagg ccatgaccaa
     6421 tataaagttg tctcgttacc gttggtatgg ttgacaccca atgtattgat actgggctga
     6481 tgaatagaac aggagttgta gtcggtttgc cagggaacag acatgaattt cgaaatgtca
     6541 ccgggttcta gaccatcggt catattgtgc atcggaatcc aaccgcccgt caggaatggt
     6601 ttgtctttgg aagcttcggc atatggcaaa tttttgtgca ttatccgaaa tggcccggcg
     6661 cccgaacttc gccagtcgtg catatatatt tcggtacagc gtacgacata acttacttca
     6721 atacccggga catatcggcc gccaaggcaa ttgctcagtg atgccatatc aagcagttct
     6781 gtctgattca acgcggctcc gtcgcctctg ataaaattat ttttactcca ttgctccaaa
     6841 aaaaagtatt gtgtttttgt cacggtaaga aaagatgtcc ccgcatcgcc cacggataat
     6901 ggcatcagtg gtacgccgat atttaattcc ggcgcgacat tcgggttgcg aataaagttc
     6961 aaaccagcca tgatggtttc attcggaaca tcttcactct taatggcatc taccgcgtcg
     7021 tgtgctctga gcgctatttt cgggagcagc gtggtccagc gctggaggtt tgccgcgcga
     7081 aagacaggtc ggatcattgt ttcaaaagcc ggttgatacg cgctgttgaa attacctgtt
     7141 tcgctgtcat aaatttcggg ttgcaaagca agctctttgt cccttatcca gacatcaaat
     7201 acatcatccc agaccgaaac aacatttcga atttgcggcg cgaaagccgg atcgccgcag
     7261 acgacccagg ctccaaaagc ggcttcggct gtgccgtccg taaaaagcag cgtcgccgat
     7321 accggcccgt cgcttgaatc atcaaaccag cccgcgttgt tcagatcgcc ggttaatggt
     7381 atcgggtcgc cgtattcatc atattgcgct gccgcgcgac caaaccccgg taataccaag
     7441 agtcgtcctt tttcgtcggt aaggatatcg cccaaactgt ccaattttcc attcggctca
     7501 aacaatttac cgccggggaa gttgatcgga tagtcaggca ggggaatgat ttccccgctt
     7561 aggctgccaa aagcggcgct gccggtttta tcaaatttcg cgcacgtttt ttcggcggcg
     7621 gaaattgctc gcgggccggg atcgataaca agttttaata aacgattggc cgagttcaca
     7681 tctccgtaaa cgtcctgatt acgcaattgc ggtactttgt cattgtaggc attaactcct
     7741 tgagaattga caacaacata ggcggcggcc tttttattgg caaggtgcgc cgtccagata
     7801 atatctttaa caactctgcc gtcacccaga tgactcccga tctcgatctc gttcacggtg
     7861 tttttggaag gataaacatc gtcaccttcc aaaacagtct caaacagacg aaagcgggcc
     7921 gcctgacgct ttaataatcc ctctttatca cgcagatcgg cgctagtgat aaaatcactt
     7981 tcggtgcccg gtttgatagg taatccgccg gtggtttccg tcccttgcag cggcatcccc
     8041 gcactggttt ccggcgataa ataatattcg ttgctggtcc cgaccctggc aaaattgatc
     8101 gagggatgaa ccctgaatat tgtttttttt gatatattat ttgacatatt atttagtgtt
     8161 taaagttaat caatcaaaag accggcgtta tctcatgctt ccaacagttt tgaatatccg
     8221 cgcccactaa aagcatcatc cggaaaaaat cctcagggtt ttcgccgtta aacagcgctt
     8281 ctaagttttt acacaaaccg ctgaaatgat tttgcaggat ttccagtagc cgccggtctt
     8341 catccgtata ttccgatatg gcttttaccg ggtaagtggc ttgaggagca gcggttttga
     8401 tccggataaa tttttcaaaa tgagataatt cctgtcgttt atcatccgcc gtattttgta
     8461 aaacttcctg tacatcttct tcagctttac tgatcccttc tccctgatcc gtaattaatt
     8521 taattaacag atcgacctgg taaaatccgc ggcggccgct ttccgtaatc gtcatcgccg
     8581 gcatatttcg ataaaaatta gaaaaaagat ccacttgttt cacaccgccg tgtaatttat
     8641 ctttcagttg atcggctcca tcgcgaagcg ccagataaaa ttcaccgata ttgccgtaat
     8701 cagtgacatc cggatttagg tcgggtttgt cacttgaatc ccaatcaggg tattcgatca
     8761 aacacatcgc attgatccgt tcgaggtcaa gcgggcctat ttcggcactg aaaggagtat
     8821 attccggcct tggatcgggc ttgtccagat caaagtttaa gtgagggaca tttttcccaa
     8881 catagactgg aacattaaaa gtcggcgaaa atccataggc gttggcaata ttggatgccg
     8941 attgcaggtg aagcatttcc tgattaaccg ccgtttgaat aaactgatat gcgtcggaac
     9001 tgcggtcttt aactgaatac atcgcactca tataaaaggg gatcgtccaa aattccagat
     9061 caaccgcata ctgaatatgc ttttgtaata aatccagtgt ccattccggg ttttggatct
     9121 gtaaaccgtt cttaatagtt tccatgatta aagtgttgat tataccggac aaggctgcaa
     9181 catcggaata tttgaagttc taaggctgtg cgctcccagc gcgagataat ttcacgcagc
     9241 cggacgcaaa gtccgcaaag aaaaccaaaa tttttgcctt gttgagtata gttaaaataa
     9301 aatgcggagc ccgttctttc aaaagaataa gattttatgg tagtgggtca ttttcaatgt
     9361 tgaaccaata aagataagaa ccttgtcttt gtattattaa ggagagaatc gctaattctc
     9421 gccatatatc gaatttttaa agtctattgg cgagttaatc actctaaatc gtgtttgtct
     9481 gtgcgataac acgcatagct aaaatcttaa ttcgtgttcg acatggaaaa tgacccgcta
     9541 ccgattttat ttttaaagct attgattttg ggtattgaag ttttcttttg ccataataaa
     9601 taattactaa aatactcatc gcagtattta aagaattagt tatcggcttt gaaaaatcat
     9661 tgaaaggtta cttatttttt gatttctttt ctaacaggtt gctgccacat tacagcaaat
     9721 tcgcaaaata catacctttt tgtgtttgag gggactataa aagattaatt tccaaaagtc
     9781 aaacaacgtc taagctcgat tttgtcgagg gttaataaaa aattgtgtaa acggcattaa
     9841 tcaacattta tttttagtgg aagattattt atgccgatta ccaatgaagt tttagaccaa
     9901 atactgggag attatgagaa gcccgaagat ttgttaagtg aaaacgcctt gttgcaacag
     9961 ttgaccaaag tacccgatcg tttatctgga tgcgctgaaa gtcaaggtca aaagcggcgg
    10021 gcgcgtcgtc aacaaatcga tttatctggc gttcggcgtg attttgagcg gctacaagga
    10081 agtcctcggc atccgggcgg ctgataccga gggtgccaag ttctggctcc aagtcgtcac
    10141 cgaattgaag aatcgcggcg tgcaggatat ttttatcgcc tgcgtcgacg gactcaaggg
    10201 cttgcccgaa gcgattgaag ccgttattcc cgcagactca agtg
//
LOCUS       DASWNE010000172         6281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_429344, whole genome shotgun sequence.
ACCESSION   DASWNE010000172 DASWNE010000000
VERSION     DASWNE010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6281
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429344"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..787)
                     /locus_tag="VGB68_03620"
     CDS             complement(41..787)
                     /locus_tag="VGB68_03620"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEY0048349.1"
                     /translation="MNFTILRFDAIDSTNTEALNQARLGAAEGLCIVARRQTAGRGRH
                     GRAWISEADAGLYFSIVLRPKIETRFLPLMTLMTAVAVHDTLEEAFNLECDIKWANDI
                     HVRDKKICGILAETADAARGLAVVVGIGINLKSSNFPPEIAEIATSIEAESAQKINSD
                     ELLEALTRFLDYHYEILHKARGAEIIRQEWKRRSSYAVGKKVRVFLENETIYGTTGGI
                     EENGALRVLTDTGETRIIQAGDVEKLRREK"
     gene            complement(936..1904)
                     /gene="egtD"
                     /locus_tag="VGB68_03625"
     CDS             complement(936..1904)
                     /gene="egtD"
                     /locus_tag="VGB68_03625"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEY0048350.1"
                     /translation="MQSSIQTQINVFAEDVGRGLSASPKFLSSKYFYDDEGSRLFQEI
                     MKLPEYYLTRAEFEIFSTRTREIFEAFAARNASFDLIELGAGDGTKTAVLVDYFLRQN
                     ADFTYSPIDISQEALDLLSAKFKAEFPPLSMNAQNGDYFSILETLKTAAARPKIILFL
                     GSNIGNFSKAEAEDFFCRLRAATNENDLLFIGFDLQKDPRVIVRAYDDARGVTAEFNL
                     NLLRRINRELGANFDLEKFSHYALYRPNEGAARSFLISRAQQAVFVEALNQTFDFKAW
                     EPIFMEISQKYSLEMIEELANACGFQVAQNFFDSQKYFVDSLWKII"
     gene            complement(2003..3196)
                     /gene="egtB"
                     /locus_tag="VGB68_03630"
     CDS             complement(2003..3196)
                     /gene="egtB"
                     /locus_tag="VGB68_03630"
                     /inference="COORDINATES: protein motif:HMM:TIGR03440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HEY0048351.1"
                     /translation="MQGTNLKKSEAQNLVEFYEGVRQYTEKLCAPLEIEDYIPQPIAD
                     ISPPKWNIAHTTWFFEEMILKKFVPEYRVFDKDFAHFFNSYYNSVGTRTLRHKRGDLS
                     RPTVKEVFAYRKYVDERMRDFLQTDLGREARDLVVLGLNHEQQHQELFLADLKYIFAC
                     NPLFPVYDAENVREEKGEITASESAEFIEINNGIYEIGYNENGFCFDNELRRHKVYLH
                     DFSISKNLVTNGDFLEFIADGGYENFNLWHSEGWDWVRQNEIKSPLYWHANGGEWFQF
                     TLSGLRRVLPENPVCHVSFYEAAAFAEWKGSRLPTEFEWEAASAKFEWGWRWEWTNSA
                     YLPYPNFTKAAGAIGEYNGKFMINQMVLRGASVVTPLNHSRPTYRNFFHPHLRWQFTG
                     IRLAK"
     gene            complement(3293..4261)
                     /locus_tag="VGB68_03635"
     CDS             complement(3293..4261)
                     /locus_tag="VGB68_03635"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY0048352.1"
                     /translation="MKFNRRFAQINADDSNEKSASVRARLRFCFFLCAVCVLAGNSLA
                     QEITNITAEEYEWIAVEAYLQRTIKSGNTEQKRDALFRIRNLKTDEAARLAVPALSDK
                     SEIVRASAAQAVVFLPPGEAAQILLPLLRDKSILVRKEAAYALGETRNSQAVSPLVDV
                     IGRDKIQEVKDAATVALGEIGDVSAIGALTRILQRSPRDEEEFFRRAAARSIGQIAQI
                     IQINENKTITPENFLPERLKETIKPVYKNLSETFPQFRAALPVLIQALQNPREPDDAR
                     REAAFALGALGDESAIPVLRAYLNGKDYYLAEISREALLKLSRQAK"
     gene            complement(4268..4513)
                     /locus_tag="VGB68_03640"
     CDS             complement(4268..4513)
                     /locus_tag="VGB68_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048353.1"
                     /translation="MVRQTNSKIVREIDKLNENETLAVLDYISQLLSSRQLPIPSETQ
                     FNDDLIASLSEKRENRCAQQVLEWEKTRRRGHYQRAA"
     gene            4732..5016
                     /locus_tag="VGB68_03645"
     CDS             4732..5016
                     /locus_tag="VGB68_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048354.1"
                     /translation="MFIAELTEKQWLYRKFQVLAPHGSFEVLYNGWGLGYEEILVNGE
                     IACRLSSLWYVPEFKFKIGNADAKINVSVWAWMQIRSFDFEIEGESVYSE"
     gene            5308..>6281
                     /locus_tag="VGB68_03650"
     CDS             5308..>6281
                     /locus_tag="VGB68_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY0048355.1"
                     /translation="MEEKYFAQEVEEKWQKKWRDAGAFETEIDNARPKFYQLEMLPYP
                     SGNLHMGHVRNYAIGDALAWYKRLKGFNVLHPIGWDSFGQPAEDAAIKRGVNPRDWTE
                     ENIRHMTGQLQRMGFSYDWRRQTAAHRPDYYKFNQWFFLKMYEMGLAYKKMSQVNWCP
                     NEKTTLSNEQASGGICWRCGSTVEKKDLAQWFLKTTQYADQLLDDMAEIEAGWAEKVL
                     KRQRDWIGRSEGAFVDFEVQDTNEKIRVFTTRIDTIYGANAIVVAAEHPIIEANPANL
                     SVETRAVIEKIKADNLKVVDHDVEPEKEGVDTGLRAINPFSREELPIWV"
BASE COUNT         1562 a         1536 c         1587 g         1596 t
ORIGIN      
        1 tgtcgaagcc ggcttcaaaa gccgaaatgc ggctgccgtt tcacttttcc cgcctcagct
       61 tttcgacatc gcccgcctgt atgattctcg tttcgcccgt gtcggtcagc acgcgcagcg
      121 cgccgttttc ctcgatgccg ccggtcgtgc cgtaaatcgt ttcgttttcc agaaaaaccc
      181 gcaccttttt gccgacggcg taagacgagc gccgcttcca ttcctgccgg attatctcgg
      241 cgccgcgcgc tttgtgaaga atttcgtaat gataatcgag aaaacgcgtc agcgcttcga
      301 gcagctcgtc ggaatttatt ttttgcgcgc tttcggcttc gatagaagtg gcgatttcgg
      361 caatttccgg cggaaaattc gacgatttta aattgattcc gatgccgacg acgaccgcca
      421 aacctctcgc cgcgtcggcg gtttccgcca gaattccgca gattttttta tcgcggacgt
      481 gtatgtcgtt cgcccatttt atatcgcatt cgagattgaa agcttcttcg agcgtgtcgt
      541 gaacggcgac ggcggtcatc agcgtcatca gcggcaaaaa gcgcgtctca atcttcgggc
      601 gcagaacgat gctgaaataa agcccggcgt ccgcttcgga aatccacgcg cgcccgtgtc
      661 gcccgcgccc ggcggtttgc cgccgcgcga cgatacacaa accttccgca gcgccgagtc
      721 gcgcctgatt tagggcttcg gtattcgtgg aatcgattgc gtcgaaacgt aaaatcgtga
      781 aattcattta acggcgtttt tagcgcgaac aacgtaaacg gcgcaatgag gtaaagaaag
      841 aattaaccgg cgataacggg ttttacagcg atggttaaga taaattcttt cttattccgg
      901 ttaattcttt ctttgcgcgt tttacgtctt tcgcgttaaa taatcttcca gagcgaatcg
      961 acaaaatatt tctggctgtc gaagaagttt tgggcgactt gaaaaccgca ggcgttcgct
     1021 aattcttcaa tcatctccag actgtatttc tgcgagattt ccataaaaat cggctcccac
     1081 gctttgaaat caaacgtttg attcagcgct tcgacgaaaa ccgcctgctg cgcgcggctg
     1141 atgagaaacg agcgcgccgc gccttcgttc gggcgataaa gggcgtaatg cgaaaatttt
     1201 tccaaatcga aattcgcgcc cagctcacgg ttgatgcggc gcagtagatt taaattaaat
     1261 tcggcggtca cgccgcgcgc gtcgtcgtag gcgcggacga tgacgcgcgg gtctttctgc
     1321 aaatcgaagc cgataaaaag caaatcgttt tcattcgtcg cggcgcgcaa ccggcagaaa
     1381 aaatcttcgg cttccgcttt gctgaaattg ccgatattcg agcccagaaa cagaatgatt
     1441 ttcgggcgcg ccgcagcggt ttttaaagtc tccagaatgg aaaaataatc gccgttttgc
     1501 gcgttcatcg aaagaggcgg aaactcggct ttaaattttg ccgaaagcaa atccagcgct
     1561 tcctgcgaga tatcaatcgg cgagtatgtg aaatccgcgt tctgccgcaa aaaataatcg
     1621 accagcacgg cggttttcgt gccgtcgccc gcgcccagtt cgattaaatc aaacgaagcg
     1681 tttcgcgccg caaacgcttc gaaaatctcc cgcgtccgcg tcgaaaaaat ctcgaattcc
     1741 gcgcgcgtca ggtaatattc gggcagcttc attatttcct gaaacagtct tgagccttcg
     1801 tcgtcgtaaa aatatttaga cgataaaaat ttcggcgagg cggacaatcc gcgcccgacg
     1861 tcttcggcaa aaacgttgat ttgagtttgt attgaacttt gcatcgtttt aaattcagga
     1921 ttcaggattc aagattcaag attcaagatt caagattcaa gattgaggat tcagaattga
     1981 attttaaatc ctgaatcctg aatcatttcg ccaacctgat gcccgtaaac tgccagcgca
     2041 ggtgcgggtg gaaaaaattt cgataagtcg ggcggctgtg attcaaaggc gtcacgacgc
     2101 tcgcgccgcg cagaaccatc tggtttatca taaacttacc gttgtattcg ccgatggcgc
     2161 ccgccgcttt cgtaaaattc gggtacggca gatacgccga attcgtccat tcccagcgcc
     2221 agccccattc gaatttcgcg ctcgccgctt cccattcgaa ctcggtcggc aggcgcgagc
     2281 ctttccattc ggcaaacgcc gccgcctcgt aaaacgaaac gtgacagacc ggattttccg
     2341 gcaatacgcg ccgcaagccg ctcaaggtaa actgaaacca ttcgccgccg ttcgcgtgcc
     2401 agtaaagcgg gcttttgatt tcgttctgcc gaacccagtc ccagccttcc gagtgccata
     2461 aattaaaatt ctcgtagccg ccgtccgcga taaattccag aaaatcgccg ttcgttacaa
     2521 ggtttttact aatcgaaaaa tcgtgcaggt aaactttgtg gcgtcggagt tcgttgtcga
     2581 agcagaaacc gttttcgttg tagccgattt cgtaaattcc gttatttatt tcgataaatt
     2641 cagcgctttc ggatgcggta atttcgcctt tttcttccct gacgttttcc gcatcgtaaa
     2701 ccgggaagag cgggttgcag gcgaagatgt atttcaaatc cgcgagaaaa agctcctggt
     2761 gctgctgctc gtgattcaag cctaaaacaa ccaaatcgcg cgcctcccgt cccaaatcgg
     2821 tttgcagaaa atcgcgcatt cgctcgtcga cgtattttcg ataggcgaaa acttccttga
     2881 cggtcgggcg cgacaaatcg ccgcgtttat gacgcaaggt gcgcgtgccg acggaattgt
     2941 aataactgtt aaaaaagtgc gcgaagtctt tgtcgaaaac gcggtattcg gggacgaatt
     3001 tttttaaaat catttcctcg aaaaaccacg tcgtgtgggc gatgttccat ttcggcggcg
     3061 agatgtcggc aatcggctgc ggaatgtaat cttcgatttc gagcggcgcg cagagctttt
     3121 ccgtgtattg acggacgccc tcataaaact cgactaaatt ttgagcttcg gattttttga
     3181 gattcgtgcc ttgcatagac tgattataaa tcagaaagcc gccgaagaaa cgttaaatct
     3241 tttagaattt aaaaaaatat ttcctgattg agtatcagga acgaaccgca ttttatttcg
     3301 cctgtcttga aagtttcagc aatgcttcgc gggaaatttc ggcgagataa taatctttgc
     3361 cgttcaaata agcgcgcaaa accggaattg ccgattcgtc gccgagcgcg ccgagcgcga
     3421 aagccgcttc gcgccgggcg tcgtccggtt cgcgcggatt ttgcagagcc tgaatcaaaa
     3481 ccggaagcgc ggcgcgaaat tgcggaaacg tttcggaaag atttttgtaa accggcttga
     3541 ttgtttcttt taaccgttcg ggcagaaagt tttcgggcgt gattgttttg ttttcattta
     3601 tctggataat ctgcgcgatt tgtccgattg agcgcgccgc cgcgcgccgg aaaaattctt
     3661 cctcgtctcg cggacttcgc tgcaaaatcc gcgtcaaagc gccgattgcc gaaacgtcgc
     3721 cgatttcgcc cagcgcgacc gtcgcggcgt ctttcacttc ctgaatctta tcgcgcccga
     3781 tgacatcgac cagcgggctg acggcttggg aattgcgcgt ttcgccgagc gcgtaagccg
     3841 cttccttgcg aaccagaatc gatttatcgc gcaaaagcgg cagcagaatt tgcgcggctt
     3901 cgccgggcgg aagaaaaact acggcttgcg cggcggaagc gcgaacgatt tcggatttat
     3961 cggataaagc gggaacggca aggcgcgcgg cttcgtcggt tttcagattg cggattcgga
     4021 aaagcgcgtc gcgtttctgc tctgtatttc ctgatttaat tgttctttgc agatatgctt
     4081 cgactgcaat ccactcgtat tcttcagcgg ttatattagt aatttcctgc gcgagggaat
     4141 ttcccgccag gacgcaaacg gcgcaaagaa aaaaacaaaa ccgcagacga gcgcggacag
     4201 acgcggattt ctcgtttgaa tcatctgcgt ttatctgcgc gaatctgcgg ttaaatttca
     4261 taaattttta agccgctctc tgataatgcc cgcgccgccg cgtcttttcc cattcgagaa
     4321 cctgctgcgc gcagcgattc tcgcgctttt cggacaaaga cgcaatcaag tcatcattaa
     4381 attgtgtttc gctcggaatc ggaagctggc ggctggaaag aagctgggaa atgtaatcaa
     4441 gaacggctaa agtttcgttt tcgtttaatt tatcaatttc gcggacgatt ttgctgttag
     4501 tttgtctgac catctcttgc actccttttt ttcggagcag acagtcagat gcaaacttta
     4561 aaatttcaag ttttgactgt ctgcaaaatt gtttttctca tcattaaatc tttcgtggaa
     4621 gtaaaactcc taaattttag gcttttttca aaatagcata aaattattat tcgaggcaag
     4681 attttgataa aattccgacg gaaaatttaa gttttataat tataatccga tatgtttata
     4741 gcagaattaa cggaaaaaca atggctttac cgaaaatttc aggtgctcgc gccgcacggc
     4801 agcttcgagg ttctttataa cggctgggga ttgggttacg aagaaattct ggtcaatggc
     4861 gaaatcgcct gccgcctgag cagcttgtgg tatgtgccgg aatttaaatt caaaatcggg
     4921 aatgccgacg cgaaaataaa cgtcagcgtc tgggcttgga tgcagattcg ctcgttcgat
     4981 tttgagattg aaggcgaatc ggtctactcc gagtaaatcg agaaactgga caaaccgtca
     5041 atattgattt aaaattcgcg tatgatttcg tttcgcaatc taaccaaact cctccttagc
     5101 tagccacccg ccggtggttt gttttccggc gcgcgtggcg cagcatatcg aattaaatca
     5161 atttaaaatc aggaattaac gcgtttagtg gatagatttt tatttattcg ctagacttac
     5221 ttttttgagt cgggtatcga gtgtgaagag tcggggaaaa tccctttaat tcttcactgt
     5281 caggtttccg gctccggact aaagtatatg gaagagaaat attttgcaca ggaagtagaa
     5341 gaaaaatggc agaaaaaatg gcgcgatgcg ggcgcattcg agaccgagat tgataacgcg
     5401 agaccgaagt tttatcaact ggaaatgctg ccgtacccgt cgggcaatct gcatatggga
     5461 cacgtccgca attacgctat cggcgatgcg ctggcgtggt ataaacgcct gaaaggcttt
     5521 aacgttctgc acccgatcgg ctgggattcg ttcggtcagc ccgccgaaga cgcggcgata
     5581 aaacgcggcg ttaatccgcg cgactggacg gaagaaaata tcaggcatat gaccgggcag
     5641 ctccagcgta tgggtttcag ctacgactgg cggcggcaga cggcggcgca tcgaccggat
     5701 tattacaaat tcaaccagtg gttttttctg aaaatgtatg aaatgggctt ggcgtataaa
     5761 aaaatgtcgc aggtcaactg gtgcccgaac gaaaaaacga cgctctcgaa cgagcaggcg
     5821 agcggcggaa tctgctggcg ctgcggcagc acggtcgaga aaaaagattt ggcgcagtgg
     5881 tttttgaaaa ccacgcagta tgccgaccag ctgctggacg atatggcgga aatagaagcg
     5941 ggctgggcgg aaaaagtctt gaagcgccag cgcgactgga tcggacgctc ggaaggcgcg
     6001 ttcgtcgatt tcgaagttca ggacacgaac gaaaaaattc gcgtctttac cacgcgcatc
     6061 gacacgattt acggcgctaa cgcgatcgtc gtcgcggctg aacatccgat tatcgaagcg
     6121 aatccggcaa atctttccgt cgaaaccaga gccgttatcg aaaaaatcaa agccgacaat
     6181 ctgaaagtcg tcgaccacga cgtcgaaccg gaaaaagaag gcgtcgatac gggcttaaga
     6241 gcgataaatc cgttcagccg cgaagaattg ccaatctggg t
//
LOCUS       DASWNE010000173         4438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_40982, whole genome shotgun sequence.
ACCESSION   DASWNE010000173 DASWNE010000000
VERSION     DASWNE010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40982"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="VGB68_03655"
     CDS             complement(<1..884)
                     /locus_tag="VGB68_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY0048356.1"
                     /translation="MENLSNEQPNLDVLILAAGLGTRMRSNQAKVLHRLDGRPLINHV
                     CRAATALAPRKVYVVIGHQGEDVKRAVLEEMDANHVEFVWQNEQLGTGHAVNSARRFL
                     ENEDSILMVLSGDVPMIRAGTLAGIVRQHQTHRGRGAAGTLLTVKMDDPTGYGRIVRD
                     DAGLFHKIVEQKDATGDEKKIREINAGIYCFNTKKLFSALSRVKNENAQGEYYLTDVP
                     ELLRADGEDVAIYAHSDAHEIEGINNRVQLADMERVLRHRIVRRLMLDYGVSFINPQA
                     AYISEQASIGRDTIIYPNV"
     gene            complement(1034..2059)
                     /gene="yvcK"
                     /locus_tag="VGB68_03660"
     CDS             complement(1034..2059)
                     /gene="yvcK"
                     /locus_tag="VGB68_03660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HEY0048357.1"
                     /translation="MKNHLFSHDSNGLNLVAIGGGNGLSTLLCGLKRFVDAGESEPIW
                     LEKLSAIVAVSDDGGSSGRLRDELQMLPPGDIRNCMVALSEDSSLLSKLFQHRFRGDG
                     ELGGHSFGNLFLAALSEITGDFAEAVKLSSEILASKGHIYPATVADVRLVAELTDGRI
                     VKGETNIAKFGTAIRRLRLEPEKCNPLPEALAAIYEADVITIGPGSLYTSLLPPILVE
                     GVAEAIAEAAAVRIFVCNLMTQPGETDGYSARRHLEIVRQYAPQINFDYVVVNNHLIN
                     EQQSARYASEGAEQIGVHGSVSPETIEGAEIVCGNLLDEGEMVRHHPEKLAQVALLCA
                     AQPKVLV"
     gene            2451..2933
                     /locus_tag="VGB68_03665"
     CDS             2451..2933
                     /locus_tag="VGB68_03665"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEY0048358.1"
                     /translation="MAETPFIIQEQQFQKIKTVLARLCVESAARVVFLVDRDGQPIAF
                     HGDIGDMDTTSFSSLAAGNVAATSSMAKLIGEDVFPAVVHEGEHESIYICVIGRSLLV
                     VVFNERSTLGLVKLRAKRASHEVAALIDEMVRHSEQNKAQSNAFFAEITDEDIDSLFS
                     "
     gene            3010..3594
                     /locus_tag="VGB68_03670"
     CDS             3010..3594
                     /locus_tag="VGB68_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HEY0048359.1"
                     /translation="MTFINYASREINCKIVYYGPGLCGKTTNLQYIYDSTAPTAKGKL
                     ISLATETDRTLFFDFMPLELGTVRGFKTRFHLYTVPGQVYYDASRKLILKGVDGVVFV
                     ADSQEERMDANIESLYNLEENLQSQGYNLMDLPYVLQLNKRDLPNVIPTDELAAELQR
                     KGEPVFEAVAAKGDGVFDTLKAVAKQVLTELRKG"
     gene            3687..3893
                     /locus_tag="VGB68_03675"
     CDS             3687..3893
                     /locus_tag="VGB68_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048360.1"
                     /translation="MNGKAFSTGWKILNALLGLFFLYSIIDAWIQILYFKNPEKHYYY
                     IGTTFFIPLFTCLVVVCGWSLEKP"
BASE COUNT         1108 a         1131 c         1114 g         1085 t
ORIGIN      
        1 acgttcgggt aaattatcgt gtcgcgcccg atgctcgcct gctcgctgat atacgccgcc
       61 tgcggattga taaagctgac gccgtaatcg agcatcagcc ggcggacgat gcgatgacgc
      121 aaaacgcgct ccatatcggc aagctggacg cggttgttga tgccttcgat ttcgtgcgcg
      181 tcggagtgcg cgtaaatggc gacgtcttcg ccgtcggctc tcaaaagctc cggcacgtcc
      241 gttaaataat attcgccctg cgcgttttcg ttttttacgc gcgaaagcgc gctgaaaagc
      301 tttttcgtat tgaagcagta aatcccggcg ttgatttccc tgattttctt ttcgtcgccg
      361 gtcgcgtctt tctgctcgac gattttgtgg aaaagcccgg catcgtcgcg gacgattctg
      421 ccgtagccgg tcgggtcgtc cattttaacg gtcagcagcg tgccggcggc tccgcgcccg
      481 cgatgcgtct ggtgctggcg gacgattccg gcgagcgtcc cggcgcgaat catcggcacg
      541 tcgccggaaa gaaccatcaa aatcgaatct tcgttttcca gaaaccggcg cgcggaattg
      601 acggcgtgac cggtgccgag ctgctcgttt tgccagacga actcgacgtg attggcgtcc
      661 atctcttcga gaaccgctcg tttgacgtct tcgccctgat gcccgatgac gacataaact
      721 ttgcgcggcg cgagcgcggt cgccgcgcgg cagacgtggt taatcaaagg acgtccgtcg
      781 agccggtgca gaaccttcgc ctggttcgag cgcattcgcg tgcctaatcc ggcggcgaga
      841 atcaggacat cgagattcgg ttgttcgttg gacagatttt ccatactcac aaaaatttta
      901 acgcaaagac gcaaaggtgc aaggtcgcaa aggcaattta agtaaaaagg caaaagtaaa
      961 aaggcaaaaa aaataattcc gaacttttta cttttacctg tttacttttg ccctgcaatc
     1021 tcgcgagtta aaactaaacc aaaaccttcg gctgcgcggc gcagagcagt gcgacctgcg
     1081 ccagtttttc gggatgatga cgaaccatct cgccttcgtc caaaagattt ccgcaaacga
     1141 tttccgcgcc ttcaatcgtc tcgggcgaga ccgagccgtg aacgccgatt tgctcggcgc
     1201 cttccgaggc ataacgcgcc gattgctgct cgttaatcaa atgattgttg acgacgacgt
     1261 aatcgaaatt tatctgcggc gcgtattggc gaacgatttc cagatgccgc cgcgccgaat
     1321 aaccgtcggt ttccccgggc tgcgtcatca ggttgcagac aaaaattctc accgccgcgg
     1381 cttccgcaat cgcttccgcc acgccttcga ccagaatcgg cggcagcaga ctcgtataaa
     1441 gcgagccggg accaatcgtg atgacgtccg cttcgtaaat cgccgccaaa gcttcgggca
     1501 gcggattgca tttttccggt tcgagccgca ggcggcgaat cgcggtgccg aatttggcaa
     1561 tgttcgtctc gcctttgacg attcttccgt ccgtcagctc ggcgacgagc ctgacatcgg
     1621 cgacggtcgc cgggtaaatg tgacctttgc tcgccagaat ttccgacgaa agcttgacgg
     1681 cttcggcaaa atcgccggtg atttcgctga gcgccgcgag aaaaagattg ccgaagctgt
     1741 gtccgccgag ctcgccgtcg ccgcgaaaac gatgctggaa aagcttcgat aaaagcgacg
     1801 aatcttccga cagcgcgacc atacagttgc gaatgtcgcc gggcggcagc atctgcaatt
     1861 cgtcgcgcag cctgccgctg cttccgccgt cgtcggagac ggcgacgatt gccgaaagct
     1921 tttccagcca gatcggctcg ctctcgcccg cgtcgacaaa tctttttaag ccgcagagca
     1981 gcgtcgaaag cccgttgccg ccgccgatgg cgactaaatt caagccgttc gaatcgtgtg
     2041 aaaaaaggtg gtttttcatt ttttttcgcg gtgcgggctg gtttttaaat agtttaaaaa
     2101 aggcgggtca gagcagtttc caacgcggca tttatctttc ggcgtatttt atatccttat
     2161 atttcgggtt ttcagcgagc acgccggttt ccacgagaga aagtttagca cttttgcgat
     2221 tcggtgagca atagcttttt ctgataaccg ccacagcgaa aagttatcgg acaaaaacaa
     2281 aaaaaatact tgaagccaaa ccgcggttat gctagattaa aaatgcggtt tatcgcaaga
     2341 cccgccctgg taaatcgtct aaaccaattt tcaaaaataa aattagagtg aatttgacaa
     2401 aattcctccc tcgttttacc aataataaac agaagaagag ctaaaaactc atggcagaaa
     2461 cgccttttat catacaagaa cagcagtttc aaaaaattaa aaccgtgctt gcgcggctct
     2521 gtgtcgaatc ggcggcgcgc gtcgtttttc tggttgaccg cgacggtcag ccgatagctt
     2581 ttcacggcga catcggcgat atggacacga cgagtttttc gagcctcgcc gccggcaacg
     2641 tcgccgcgac gtccagcatg gcgaagctta tcggcgagga cgtttttccg gcggtcgttc
     2701 acgagggcga acacgaaagc atctacatct gcgttatcgg cagaagcctt ctggtcgtcg
     2761 tctttaacga acgctcgacg ctcggcttgg taaaattgcg cgccaaacgc gccagccacg
     2821 aagtcgccgc gctgatagac gaaatggttc ggcattccga gcagaacaaa gcccaaagca
     2881 acgcgttttt tgcggaaatt accgacgagg atattgacag cctgtttagt taaaagtgcc
     2941 gagtgccgag tgctgagtcg tgatttaaaa ctcagccctc ggcactcggc actcagcact
     3001 ttaaaagata tgacattcat caactacgct tcacgcgaaa tcaactgcaa aattgtttat
     3061 tacggtcccg gtttgtgcgg caaaacgacg aacctgcaat acatctacga ttcgaccgcg
     3121 ccgaccgcga aaggcaagct tatcagtctg gcgacggaga ccgaccgcac tctgtttttc
     3181 gattttatgc cgcttgagct cggcacggtg cgcggtttta aaacgcggtt tcatctttac
     3241 accgttcccg gtcaggttta ttacgacgca tcgcgcaagc tgattttaaa aggcgtggac
     3301 ggcgtcgtct ttgtcgccga ctcgcaggaa gagcgaatgg acgccaacat cgaatcgctc
     3361 tacaacctgg aagaaaatct ccagtcgcag ggttacaacc tgatggattt gccgtacgtt
     3421 ctgcaattaa acaagcgcga tttgccgaat gtcatcccga ccgacgagct agccgccgag
     3481 ctgcagcgca agggcgagcc cgtcttcgaa gcggtcgccg ccaaaggcga cggcgttttc
     3541 gatacgctga aagccgtcgc gaaacaggtt ctgaccgagc tcagaaaagg ttaatttttc
     3601 aatcgaccaa aagttcagcg aagagtttcg gctcttcgct tttttttgtt ttgttcgtaa
     3661 aatatctttt tagacgcgga aagattatga acggaaaagc attctctacc ggctggaaaa
     3721 tcctcaacgc ccttttaggc ttgttctttt tgtattcgat tatcgacgcg tggatacaga
     3781 ttctctattt caaaaacccg gaaaaacatt actactacat cggcacgaca tttttcattc
     3841 cgctttttac gtgtttagta gtcgtctgcg gctggagttt ggagaaaccg tgaaaatcgt
     3901 cggcgaagat aaaagccggt cgaatttatt tgttgtttat tacttttata gaggattaat
     3961 ccaactcttc acaagcttta tttaagcgtg ccgggttatt taatgaagag gaaaaaaaga
     4021 gatttcttaa cgccgggcat ttatctttgt cagatgcatt cctcgagccg ggattattta
     4081 atgaaaatga atcggaaaag caggtggttt tgaaaggctg cggggcgaat caagacctct
     4141 tttagaaaag caaacaaaaa taaaattccc gaccgatttt atagtctttc caaaacttgt
     4201 ttttttattt taaagtggta atatcaatca cccgtaacag aaaaaacgct cagattttat
     4261 aaagaattat ggatacctta gaacttacca gagaagaatt gattgccgaa ccggaacagc
     4321 gcaacctggc gacgggcaaa agcattttgt cttttaacgt gaaattatcg gacaaaatca
     4381 taaatttgaa tacaggcaat aaattccatt accagaacga atcatacgag ataatgaa
//
LOCUS       DASWNE010000174         3871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_76543, whole genome shotgun sequence.
ACCESSION   DASWNE010000174 DASWNE010000000
VERSION     DASWNE010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76543"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VGB68_03680"
     CDS             complement(<1..396)
                     /locus_tag="VGB68_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048361.1"
                     /translation="MEASVLKNEFNFNVYNALWNVEEADRQVNRNDRKDKADFLKKSS
                     YLLAKYNLRDKLGVRLLHKHSDIKAGELMIESEGIVNDEKSLITEPIKNPSGGKAIPS
                     VWKIVDGDFYPLEYTTDALACRLYNSLELP"
     gene            862..>3871
                     /locus_tag="VGB68_03685"
     CDS             862..>3871
                     /locus_tag="VGB68_03685"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEY0048362.1"
                     /translation="MSIPEKDLQDVRNLHEQSLHLQAFNLAQSICPLPRWEGTEAVLL
                     ASHLAYNLGALKTSVKWTTRAWRNDKKHPRAMFYYASEILNSKGSLPGLIFLRKQAAD
                     FQAEGKILSWWYCLHAGAFSFLRDFTTADEWHKKAIEAAPEEPWVWVSQAHSLEMQDR
                     YEEALAMSREAFEIKPWQRSSVFALAHILTLLERYDEALEILNEAATRLENAWVVKQL
                     ADLQSELGLYKEAYASLEKIVELTPMREDELEQWLYANLSDAAYLNGDIKKSIRFADI
                     SSEKFHLKIKENLESLKGTEKRVLLKLGFIRQHHVTCAPATISNIARFWQKPAEHLKV
                     ADEMCYDGTPAYKERLWAEENGWSTAEFQVNWENAVELINRGVPFTLTTVHPGNGHLQ
                     AIVGYDERRRTFLVRDPYFQRLEEFLAEDLLEEQKASGPRGMALVPKEKSTELLGDLL
                     FKESRQYDFHYAVDRELEKHDRKKAGEIYAEMEKAFPQHRLTWSANWAIARYDANTLQ
                     LLEAVEGLRKQFPDDINLKLSYLSVSNEQTARPERLKTLEEFCKQEKTDPLLWQMFGY
                     ELSLDARHHTRALHWLYKTLRKLPATGLTYRAIADILWSKRRFEEATELYRLSASLND
                     KDEQFAYSYFLAARHLKQTEKALKFLRDRFERFGHRSNLPVQSLFHALRELGETVEAF
                     EALEGALEKRPADGELKLFAADAKARYGRRQEAESLLRQAEKNAPRGAWLRKAALLAE
                     MDGNFQASLANWREIAGLEPLACDAHESIAYLLAATESRKSAQDYLHELTDRFPFNRD
                     LHKLRLSWLREETGEAIAILKHLLELNPHDAWSYREFSRRLHAERKYTEALSLARAAL
                     ELDPQDSLNYWAIGLVLTELGKYDEAAEAYKNSIKLSVEADYSLANWIKICRTKEEKL
                     AALDFVRSELDKQTSFGSAIIGYREQARRLLEPETLLKELQKYHAENKNAWFSASPVI
                     WQQVDMYRYDEALELAEQATRRFPLVYQVWY"
BASE COUNT          968 a          949 c         1045 g          909 t
ORIGIN      
        1 cggcaattcc agactattat ataaccggca ggctagagca tcggtggtgt attcaagcgg
       61 gtaaaagtcg ccgtcgacaa ttttccaaac gctcggaatc gctttgccgc ccgaaggatt
      121 tttgattggt tcggtgatta aagatttttc atcatttaca attccttcgg attcgatcat
      181 aagctcgccc gctttaatat cgctatgctt atgcagtaat ctgactccga gcttatctct
      241 gagattatat ttcgctaaaa gataagaact ttttttcagg aaatcggctt tatctttccg
      301 gtcgttccgg ttaacctgcc tgtccgcttc ttcaacgttc cagagcgcgt tatacacgtt
      361 gaaattaaac tcgtttttaa gaactgatgc ttccatcttt atcctccgtt tagtgatttg
      421 tgatttaatt gttgcttttt gcaagggaag tgtaatttat ttaaacagtt atgtaaataa
      481 ttttttgcgc gcgcggttta caggttttta gccgcattaa gccgccggct gatttttgtg
      541 taattgctaa gcccaatcca agtccgaaac agcgattttc gaagagtttt tcatattaaa
      601 cattgcggtc agccggacaa gtgaccggcg atttcggagg ctcgctcttt ggggttttca
      661 atgtttaaaa acaagctctg aaagagtcgc ttttgagaca agcattgcaa tacgagattg
      721 agccgccgcc ggaaaacaaa taatcttttc aaaatattaa agtcgtgtta aattaatcgg
      781 gcgcgaaagc ccggtatttt gcgcgcgccg ggtttaagtc aaaatcattt ccatctcatt
      841 caaggaaact tctaaaaaaa aatgtcaatt cccgaaaagg atttgcagga cgtgcgcaat
      901 ctgcacgagc aaagcctgca tttgcaggca tttaatctgg ctcagtcgat ttgccctttg
      961 ccgagatggg aagggacgga agccgttttg ctggcttcgc acctggcgta taatctgggc
     1021 gcgctgaaaa ccagcgtcaa atggacgacc agagcgtggc gcaacgataa aaaacacccg
     1081 cgcgcgatgt tttattacgc cagcgagatt ctcaactcga aaggctctct gcccggactt
     1141 atctttctga gaaaacaggc tgctgatttt caagccgaag gaaagattct ttcgtggtgg
     1201 tattgcctgc acgccggggc tttttccttt ctgcgcgatt ttacgacggc tgacgaatgg
     1261 cataaaaaag cgattgaagc cgcgccggaa gagccctggg tctgggtttc gcaggcgcac
     1321 agcctggaga tgcaggaccg ctacgaagaa gcgctggcaa tgagccgcga ggcttttgag
     1381 ataaagccgt ggcagcgaag ctcggttttt gcgctggcgc atattctgac gcttttggag
     1441 cgttacgacg aggcgctgga aattttgaac gaagccgcga cgcgtctgga aaatgcctgg
     1501 gtcgtcaaac aattagccga tttgcagagc gagctcgggc tttataagga agcctacgcc
     1561 tcgctggaaa aaatcgtcga gctgacgccg atgcgcgagg atgaattgga acagtggctc
     1621 tacgcgaatt tatccgacgc cgcttattta aacggcgata ttaaaaaatc gattcgcttt
     1681 gccgatattt cctcggaaaa attccatttg aaaatcaagg aaaatctgga aagcctgaag
     1741 ggaacggaaa aacgcgtttt gctcaaactc ggctttattc gccagcatca cgtgacgtgc
     1801 gcgccggcga cgatttccaa catcgcgcgt ttctggcaaa agcccgccga gcatttaaaa
     1861 gtcgccgacg aaatgtgtta cgacggaacg cccgcctata aggagagact ctgggcggaa
     1921 gaaaacggct ggtcgaccgc cgagtttcag gtgaactggg aaaacgcggt cgagcttatc
     1981 aatcgcggcg tgccgtttac tttgacgacc gtgcatcccg gcaacgggca tctgcaggca
     2041 atcgtcggct acgacgagcg tcgccggacg tttcttgtgc gcgacccgta ttttcagcgg
     2101 ctcgaagaat ttttagccga agatttgctg gaagaacaga aggcgagcgg tccgcgcgga
     2161 atggctctgg ttccgaaaga aaaatctacg gagctgctcg gcgacctgct tttcaaggaa
     2221 agccgccagt acgattttca ttacgcggtc gaccgcgagc tggaaaaaca cgaccgcaaa
     2281 aaagccggcg aaatttacgc cgagatggaa aaggcttttc cgcagcaccg tttgacgtgg
     2341 tcggcaaact gggcaatcgc gcgctatgac gcgaacactc tgcaattgct cgaagcggtc
     2401 gaaggcttgc gcaaacagtt tcccgacgac ataaatctga aattaagtta tctgagcgtt
     2461 tcaaacgagc agacggcgcg accggaacgt ttaaagacgc tcgaagaatt ttgcaaacag
     2521 gaaaaaaccg acccgctttt atggcagatg ttcggctacg agctgagtct ggacgcgcgt
     2581 catcacactc gcgctttgca ttggctgtat aagacgctgc gaaaactgcc ggcgacgggc
     2641 ttgacttatc gcgccatcgc cgacatttta tggtcgaagc gacgttttga agaagcgacc
     2701 gagctgtatc gcctgtcggc gtctctaaac gacaaggacg agcagttcgc ctattcgtat
     2761 tttctcgccg cgcggcatct gaaacaaacc gaaaaggctt tgaaattttt gcgcgaccgt
     2821 ttcgagcgtt tcggtcatcg ctcgaatctc cccgttcaga gcttgtttca cgctctgcgc
     2881 gagctcggag aaacggtcga agcgtttgaa gcgctggagg gcgcgctcga aaaacgcccg
     2941 gctgacggcg agctgaagct tttcgccgcc gacgcaaagg cgcgttacgg cagacgtcag
     3001 gaagccgaaa gccttctgcg gcaggctgag aaaaacgctc cgcgcggagc gtggctgaga
     3061 aaggcggcgc tgctcgccga aatggacggc aatttccagg catcgctggc gaattggcgg
     3121 gaaatcgccg ggcttgagcc gctcgcctgc gacgcgcacg aaagcatcgc ttatttgctg
     3181 gcggcgaccg aaagcagaaa gtcagcgcag gattatctgc acgagctgac ggacaggttt
     3241 ccgtttaacc gggatttgca caagctgcgc ctgagctggc tgcgcgagga aacgggcgaa
     3301 gcgattgcta ttttaaaaca cctgctcgag ctgaacccgc acgacgcgtg gagctatcgc
     3361 gaattttcgc gccgcctgca cgccgaaaga aaatataccg aagctttgag cctcgcccgc
     3421 gccgcgctgg agctcgaccc gcaggattct ttgaattact gggcaatcgg cttggttttg
     3481 accgaactgg gaaaatacga cgaagccgcc gaagcttata aaaattcgat aaagctgtcg
     3541 gtcgaagccg attattcgct ggcgaactgg ataaaaatct gccgcacgaa ggaagaaaag
     3601 ctcgccgctc tcgattttgt tcgcagtgaa ttggacaaac aaaccagttt cggcagcgcc
     3661 attatcggct atcgggagca agccaggcga ttgctggaac cggagacttt gctcaaagaa
     3721 ctgcaaaaat atcacgccga aaacaaaaac gcgtggtttt cggcttcgcc cgtcatctgg
     3781 caacaggtcg atatgtatcg ctatgacgaa gcgctggagc ttgccgagca ggcgacgcgg
     3841 cggtttccgc tggtttatca ggtctggtat g
//
LOCUS       DASWNE010000175         3037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_216853, whole genome shotgun sequence.
ACCESSION   DASWNE010000175 DASWNE010000000
VERSION     DASWNE010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216853"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(467..1120)
                     /locus_tag="VGB68_03690"
     CDS             complement(467..1120)
                     /locus_tag="VGB68_03690"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0048363.1"
                     /translation="MEKLRIILAEDHETVREGLKLIVNSQADMEVVGEAGNGSAAIKL
                     ARELAPDIVVMDISMPELNGLKATKKLKQLCPQVKILTLTRHTDDAYLQQLIQAGSSG
                     YVLKQSAPTELIRAIHAISEGKSYLDPALVNKVMGGYASRAASMRGDKKNDLTDRETE
                     VLRMIAWGYSNKEIAARMEISVKTVEAHKANAMQKMDMRSRIDIVRYAILQGWLQDN"
     gene            complement(1295..1657)
                     /locus_tag="VGB68_03695"
     CDS             complement(1295..1657)
                     /locus_tag="VGB68_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0048364.1"
                     /translation="MQKKILIAEDYADIRLMMKMLVRGYGYEVIEASDGYDAIEKVRQ
                     HHPDLILMDLSMPIMNGLTATEIIRTFAGMEKVPIIAVTAYGKSFYGKAIRAGCNDVL
                     SKPLDFDNLEPLLNRYLS"
     gene            1939..2367
                     /locus_tag="VGB68_03700"
     CDS             1939..2367
                     /locus_tag="VGB68_03700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0048365.1"
                     /translation="MLHSSVPRILYIENNQDGGELTAMLKRAENRPYCVLTATTPGDA
                     LELIADEPFDLLILEHQLPEMSGVELCRRIRQTDKRTPILFCSGRTRLAECDVSLAAG
                     ATEYLVRPIAAEHFIETVRRLLNDFHRLKSSNQSSCGLTK"
     gene            2601..2930
                     /locus_tag="VGB68_03705"
     CDS             2601..2930
                     /locus_tag="VGB68_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048366.1"
                     /translation="MSSNLQSNKIKKLDKIQDGLSYSIGEPDVSIRELMNRRFILENT
                     DFDTWEKLLDAAGVSSENDLNKPDFNEFIKSHTRFDDWESMLIQSANQYSLRYEGEEE
                     TTFDIKH"
BASE COUNT          789 a          703 c          738 g          807 t
ORIGIN      
        1 tgctgaacgg aattcgcggg ttgaggcgcg cggcgaggct caaaatggtt ttgcccgtcg
       61 ttggagaatt gtgaattttg cgtttgggag aacatatctt aaaaagcccg tattttttaa
      121 tctaaccaac aaatacaaaa atatttaatc tgaaaagagg gaaataaaag gttttgagct
      181 tttcctgaaa gtcagacttc ttttatctcc gcccaagcaa cttttaagtc cagtttcggc
      241 gctaacaacg aaacgttcgt taatgccgaa ctaattcctc aaattatgaa cttgattatt
      301 caactaatat ttatctcaca atgaaaggcg aatttatatc gggaatataa gaaaaataaa
      361 tagggatttt ccctaataag gtggggagaa agtgctgagt gctgagtatt gattttcaat
      421 cgaaatttaa aattcaaaat ttaaaactca gcactcagca ctcattttaa ttatcctgaa
      481 gccagccttg caaaatggcg tagcggacga tgtcgatgcg gctgcgcata tccattttct
      541 gcatcgcgtt ggctttgtgc gcttcgacgg ttttgaccga gatttccatt cgggcggcga
      601 tttccttgtt gctgtagccc caggcaatca ttcgcagaac ttccgtctcg cggtcggtca
      661 aatcgttctt tttgtcgccg cgcatggaag cggcgcggct ggcatagccg cccattacct
      721 tgttgaccag cgccgggtcg agatagcttt tgccttcgct gatggcgtgg atggcgcgaa
      781 tcagctcggt cggcgcgctt tgtttcagca cgtagccgga agagcctgcc tggataagct
      841 gttgcagata agcgtcgtcg gtgtggcgcg tgagggtcag aattttgacc tgcgggcaaa
      901 gctgtttgag ttttttggtg gctttcagcc cgttcagctc gggcatcgag atgtccatca
      961 cgacgatgtc gggcgcgagc tcgcgcgcta gcttgatggc ggcgctcccg ttgccggctt
     1021 cgccgacgac ttccatatca gcctgcgagt tgacaatcag cttcagccct tcgcgcacgg
     1081 tttcgtggtc ttcggcaagg attatccgca atttttccat aaaataatgg gcgtcggaaa
     1141 taaataactg gataattatc tctgttaaat tgtcttttag taaagcgcga gaattataaa
     1201 actatcttta acctgttagc aaggcttttt gtcgatagaa ttctaattgt tagaagtcta
     1261 aaaaacattc aaaaactatc cagcctcgct cgccttacga taaataccgg ttcaaaagcg
     1321 gctctaaatt atcgaagtcg agcggtttgc tgagcacgtc gttgcagccc gcccgaatcg
     1381 ctttgccgta gaagctcttg ccgtaagcgg tgacggcgat aatcggaacc ttttccatac
     1441 cggcaaacgt cctgatgatt tcggtagcgg tcagaccgtt cattatcggc atcgataaat
     1501 ccattaaaat aaggtcggga tgatgctgtc tgactttttc tatcgcgtcg tagccgtccg
     1561 aagcctcgat gacctcgtag ccgtagccgc gcaccagcat cttcatcatc aatcgaatgt
     1621 ccgcgtaatc ttccgcaatg agaatctttt tctgcataaa gcctacccct aacgcctcgc
     1681 aatcgttttt cgatattgac gggcgataaa tgttcgttac cttacattgt ttatctgtct
     1741 tcaccttact cttatgaatc ccgaaagtaa agggaatctt tataagaaac actgataaaa
     1801 aaggcgaaat aaaaatcagg ttttataaaa aagtcgtaaa taattcaagt ctctgtagta
     1861 aacgtgacag aaatactttg cccgcatccg gtataaattg ttttatagaa ttacgttttg
     1921 ttgaggcagg ataaaaatat gcttcattct tccgttccgc gcattcttta tatcgaaaac
     1981 aatcaggacg gcggcgagtt gaccgcgatg ctgaagcgcg cggaaaatcg tccttactgc
     2041 gttttgacgg caaccacgcc gggcgatgcg ctggagttaa tcgccgatga gccgttcgac
     2101 cttttgattc tcgaacatca actgccggag atgtcgggcg tcgaattgtg ccgccgtatc
     2161 cggcagacgg acaagcggac gccgattttg ttctgctccg gcagaacgcg cctcgccgaa
     2221 tgcgacgtct cgctcgccgc cggcgcgacc gaatatctgg tcagaccgat tgccgccgag
     2281 cattttatcg aaaccgtcag acgactttta aacgactttc accgcttgaa atcgagcaat
     2341 caatcgtcgt gcggtctgac aaaatagccc gccggcggcg cttcgggcgg acgactgtcg
     2401 gcagagagcg gcggcgcagg aggattcgtc ataccggcga gttgcttccc gccatcgccc
     2461 ttccgcgttt tagatggcga ttaaattatt ttcggaatcg gtcaggtagt cgacagaccg
     2521 gcggcgggcg agagaataaa gtagaccgac aggcaaaatc ggtattttaa aaaggacagc
     2581 gataaacaaa ggcggcgaat atgagcagca atctgcaatc gaataaaatt aaaaaactgg
     2641 ataaaattca ggacgggctt tcttattcca tcggcgaacc ggacgtttcc atcagggagc
     2701 ttatgaatcg ccggttcata cttgaaaaca cggatttcga tacgtgggaa aagctgctcg
     2761 acgcggcggg cgtcagcagc gaaaacgatt tgaacaagcc ggattttaac gaatttatca
     2821 aatctcacac tcgtttcgac gactgggaat cgatgcttat ccagtcggca aaccaatatt
     2881 ctctgcgtta tgaaggcgaa gaagaaacaa cattcgacat taagcattga gcatttatca
     2941 tttatcgaaa gcctcgcgct ccggtaaatg ataaatgttc gatgttcgat gataaatgtt
     3001 atttcttctt tatcgggacg cgggcgaaaa cggtcgt
//
LOCUS       DASWNE010000176         8523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_146423, whole genome shotgun sequence.
ACCESSION   DASWNE010000176 DASWNE010000000
VERSION     DASWNE010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8523
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146423"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1420)
                     /locus_tag="VGB68_03710"
     CDS             complement(<1..1420)
                     /locus_tag="VGB68_03710"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0048367.1"
                     /translation="MIEIGTLLQERYLIEKQIGVGGMGAVYLAVDQRFESYVAIKQTF
                     YNNDELGEAFEREARLLNSLLHPVLPHVSDYFTEAGGHFLVMQYIEGEDLFAILKREG
                     SFPVKDVLRWTDSLLDALDYLHSQEEPIIHRDIKPHNLKITPRGDVILLDFGLAKLKS
                     DNTTGVKSVFGYSRKYSPLEQIQGTGTDARSDLFALGATAYHLLTGNPPIDVLARASE
                     IIVGNPDPLPLVSELNKDVPPSVAHILNTALALNAAGRFVSAKAMRQALENAVHEASN
                     EETAVLPEQIISPVSADGGALISHETEDFPALEAFAASAAGVSQETAENNELIVDKVS
                     FPQAEAALPVEPTLRQPTITGEVATRVVVPPRRQSRLPLAVLAALIICVGLAAGYFIN
                     TANSSNQSPPTETTQEPGLVNEPADVVIDSPTPEAIAPPLLENIEQAKTKPVAIEKTV
                     ARKENNDEPAPKTEPAEKIENPE"
     gene            1601..2779
                     /locus_tag="VGB68_03715"
     CDS             1601..2779
                     /locus_tag="VGB68_03715"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEY0048368.1"
                     /translation="MPYNKLNVSGAKADILSWVNHEMSTDEHNMLRNVSRLPCLYKHV
                     ALMPDAHLGIGSMVGSVVATKDAVIPATVGVDIGCGMMAVKTPFQSSILDGRLKDLRH
                     EIERAIPVGFNEHKESDWDASRWEGWEEFDRLHKGVQSRKTKAMRQLGSLGGGNHFIE
                     ICLDTEDNVWLMLHSGSRNIGNEVAKLHIETAKHLHKLSELPDPNLAYFVQGTEEFKN
                     YWHDLEWAQRYALKNREIMMKRLLKSFNRMFNDQKEFRPEISVNCHHNYVSPEVHFGE
                     KVYVTRKGAIRADEGMYGIIPGSMGAKSFIIKGLGNPQSFNSCSHGAGRKMSRTAAKK
                     AFSREDLERQTAGVECRKDAGVIDEIPSAYKNIDEVMRNQSDLVEVVAQLKQVVCVKG
                     "
     gene            2925..3002
                     /locus_tag="VGB68_03720"
     tRNA            2925..3002
                     /locus_tag="VGB68_03720"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2961..2963,aa:Gly,seq:gcc)
     gene            3181..3372
                     /locus_tag="VGB68_03725"
     CDS             3181..3372
                     /locus_tag="VGB68_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048369.1"
                     /translation="MTEDSHALSEHSQLSNEDSQMTNEDSQLLNEHSQLLNEHSQVTN
                     EDSHVMNKDSHVFDRNSPG"
     gene            3600..4127
                     /locus_tag="VGB68_03730"
     CDS             3600..4127
                     /locus_tag="VGB68_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY0048370.1"
                     /translation="MNFSYEPLGTVGIARSMCLWFRGSVTVEEYDGDNVLHSPRETFR
                     VPSVVRLRHYVNVHRRRRETTMKRARIYIRDRYRCQYCGDHKHAADLTLDHILPRAQG
                     GESTPQNLVTACVKCNGRKGNRTPEQARMPLLTSQKLLNLGLDRVLLCHYAESKPEWK
                     KYLFMEEFEEQTRAA"
     gene            4306..4380
                     /locus_tag="VGB68_03735"
     tRNA            4306..4380
                     /locus_tag="VGB68_03735"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4341..4343,aa:Arg,seq:tct)
     gene            4549..5097
                     /locus_tag="VGB68_03740"
     CDS             4549..5097
                     /locus_tag="VGB68_03740"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEY0048371.1"
                     /translation="MKLVFIYGSPAVGKLTVANEIARKTDFKVFHNHLSIDAIEPVFE
                     FGSPSFGKLIDFIRVETVKEAARVGLNLIYTFCYAKGSDDKHVEKIARAVEEHGGEVC
                     FVLLKADRAELNKRVVEESRRAMGKAKTVEMMDFFHETYDLLSPVPDRESLIIDNTHL
                     SSENAAERIIEHFNLQQQQKII"
     gene            5223..5663
                     /locus_tag="VGB68_03745"
     CDS             5223..5663
                     /locus_tag="VGB68_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048372.1"
                     /translation="MSKKYETEDEILALVESFENGAIAREAWNHAGHLTVALYYCAHH
                     DLAAATEKMRRNLLHHLKAVGVDLTKEMPYHETLTAFWMRTVNDFRTSKNGAPLLVTA
                     NELIAGFDKDYPLRFYSRELLFSDEARARFVEADLPENNLSNQN"
     gene            complement(5730..6266)
                     /locus_tag="VGB68_03750"
     CDS             complement(5730..6266)
                     /locus_tag="VGB68_03750"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY0048373.1"
                     /translation="MAIFPLPIIPQADYHYSGIAFGAQTKNRGTRRHAACDLIAPPGT
                     PVYAVERGVVLEVPKKPFYQSVYTVVIRHNNFIIRYTELDENRPVAVGEEVYEGQQIG
                     TVGKNNKDRGMLHLEMYKGTASGPLSQEHNMNYLYVEPLNYQRRKDLIDPTPYLDEWR
                     LWTDFSVYQTEEPANSSE"
     gene            6424..6990
                     /locus_tag="VGB68_03755"
     CDS             6424..6990
                     /locus_tag="VGB68_03755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0048374.1"
                     /translation="MSVPNTQEVTELLQKWSAGDAGALDELTPVIYSELHRIARRYMS
                     RERENHTLQTTALVNEAYMRLIDWKTAKWENRAHFFGVSAQLMRRILVDFARSRPKVK
                     DEVVHHVSLEEAFVVSAEKDADLVALDEALNELAKFDERKAKIVELKFFGGLSVEETA
                     EVLRIAPITVMREWQKAKAWLYRELSKK"
     gene            7124..>8523
                     /locus_tag="VGB68_03760"
     CDS             7124..>8523
                     /locus_tag="VGB68_03760"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0048375.1"
                     /translation="MNTTRWKQIDELFDAVLDLPETEREKFLSERCSGDEELRRELLS
                     LLDAQKESEKNFLQKSAMNIAAKNLAEDQAIHFDAHFLNKTIGTYKIESLLGAGGMGE
                     VYLADDAKLRRKVALKILPSEYNTSDERVKRFELEARAVSALNHPNIVTIYDVGSFEG
                     INYIATEFVEGKTLREVIGSHLKLKEILSIIIQACDALAAAHSAGVIHRDIKPENIMV
                     RPDGYVKILDFGLAKLSEIDLHTLRDFAQTAQGVIIGTPAYMSPEQVSDENLDHRTDL
                     WSIGVVLYELLTGVNPFKKENRQATFQAILSSDPPPASSLNAEISAELDRILTKALEK
                     DAELSYQTASDFRADLKRVKREIDSSPSSWSVRSAEAETLRRRKDAAETRKNYLVYVL
                     GALVLTAIGIGVWLFVKNGRAPQNREASEWANARHAQITDSPWVEGYPSLSPDGKNII
                     FASEANNDRNIYLQRVG"
BASE COUNT         2210 a         2120 c         2141 g         2052 t
ORIGIN      
        1 tttccgggtt ttctattttt tcggcgggct ctgttttagg agccggctcg tcattatttt
       61 ctttcctggc gacagttttt tcgattgcca caggtttggt cttagcctgc tctatatttt
      121 ccagcaaagg cggagcaatc gcttcgggcg tcggcgaatc tataacgacg tcggcgggct
      181 cgttaaccaa gcccggttcc tgagtcgtct cggtcggcgg agattggtta gatgaattcg
      241 ccgtgttgat aaaatatcca gccgccagcc ccacacaaat tatcaatgcc gccagcaccg
      301 ccagcggaag ccgcgattgt cttcgaggcg ggacaacaac cctcgtggca acctcgcccg
      361 tgatggtcgg ttgccgcaag gttggttcca cgggcaaagc cgcttcagcc tgcggaaacg
      421 aaactttatc gactattaat tcattatttt cagccgtttc ctgagaaacg ccggcggcgc
      481 tggcggcaaa ggcttccagc gccggaaaat cttcggtttc atgtgaaatt aaagccccgc
      541 catcagcgct gacaggcgat ataatttgtt ccggcaaaac cgctgtttct tcgttcgaag
      601 cttcgtgaac ggcattttcc agagcctgcc gcatcgcttt cgccgatacg aaacgtccgg
      661 cggcgttaag cgccagcgcc gtgttcagaa tatgcgccac gctcggcgga acatctttat
      721 taagttcgct tacgagcgga agcgggtctg gattcccgac tatgatttcc gatgcgcggg
      781 cgagaacatc aatcggcgga ttgccggtca aaagatgata cgcggttgct cccagcgcaa
      841 ataaatccga gcgcgcgtcg gttccggttc cctgaatttg ttcgagcgga gaatatttac
      901 gcgaataacc gaaaacgctt ttcacgccgg tcgtgttatc ggatttcagc tttgccagtc
      961 cgaaatcgag cagaatgaca tcgccgcgcg gcgttatttt caaattatga ggttttatat
     1021 cgcgatgaat aatcggctct tcctgcgaat gcagataatc gagcgcgtcg agcaggctgt
     1081 cggtccagcg caaaacgtct ttaacgggaa atgagccttc gcgctttaaa atcgcaaaca
     1141 aatcttcgcc ttcgatatac tgcattacga ggaaatgacc gcccgcctcg gtgaaataat
     1201 ccgaaacgtg tggcagaacc ggatgcagca ggctgttcag aagccgcgct tcgcgctcga
     1261 aagcctcgcc cagctcgtca ttattgtaga aggtttgttt aatcgccacg taactttcaa
     1321 aacgctggtc gacggcgaga taaaccgcgc ccattccgcc gacaccgatt tgtttttcta
     1381 ttaagtagcg ttcctgtaaa agtgttccga tttcaatcat ttatttaccc atccccgtga
     1441 cagcaatttt gatgcctttc ctgacccaag cgttgataca aaaaataaat gcggaaattt
     1501 ttcggtttcg cagcttttaa attgcggcag gataattgcc tgaattttcg tacaggcgaa
     1561 acttttttta ttgacttgtt ataattttat tggaggaaat atgccataca acaaactaaa
     1621 cgtcagcggc gcgaaagccg atattctttc ctgggtcaac cacgaaatgt ccaccgacga
     1681 acacaatatg ctgcgcaacg tctcgcgcct gccgtgtctt tataaacacg tcgcgctgat
     1741 gccggacgcc catctcggca tcggctcgat ggtcggctcg gtcgtggcga ccaaagacgc
     1801 cgttattccg gcaaccgtcg gcgtcgatat cggctgcggg atgatggcgg ttaaaacgcc
     1861 ctttcagagc tcgattctcg acggcagact gaaagatttg cgtcacgaaa tcgaacgcgc
     1921 gattccggtc ggctttaatg aacacaagga aagcgattgg gatgcttcgc gctgggaagg
     1981 ctgggaggaa ttcgaccggc ttcacaaagg cgttcagtcg cgtaaaacaa aagcgatgcg
     2041 acagctcggc tcgctcggcg gcggaaatca ttttattgaa atctgcctcg atacggaaga
     2101 caacgtctgg ctgatgctgc attccggttc gcgcaatatc ggtaacgaag tcgccaaact
     2161 gcacatcgag acggcgaagc acctgcataa attgtcggaa ttgcccgatc cgaatctcgc
     2221 ctattttgtc cagggaaccg aagagtttaa aaactactgg cacgatttgg aatgggcgca
     2281 gcgttatgct ttaaaaaacc gcgagattat gatgaagcgt cttttaaagt cgttcaaccg
     2341 tatgtttaac gaccagaagg agtttcgtcc tgaaatctcg gtcaactgtc accacaatta
     2401 cgtcagcccg gaagtgcatt tcggcgaaaa ggtttacgtt acgcgcaagg gcgcgattcg
     2461 tgccgacgaa ggaatgtacg gcattattcc cggctcgatg ggcgcgaaat cgttcatcat
     2521 caaaggcttg ggaaatccgc agagctttaa ttcctgctcg cacggcgcgg gacgaaaaat
     2581 gtcgcgcacg gcggcgaaaa aagcgttttc gcgcgaggat ttggagcgcc agacagcggg
     2641 cgtcgaatgc cgcaaggacg cgggcgtgat tgacgaaatc ccttcggcgt ataaaaacat
     2701 cgacgaagtg atgcgcaacc aatcggattt ggtcgaagtg gtcgcgcaat taaagcaggt
     2761 cgtttgcgtg aaagggtaaa aaagtttgcg ggaagttgcg ggaagttgca gagagttgca
     2821 ggtagttttc agaatgtttt aaaagttagt gaaaatcgaa actaactatc tgcaacttcc
     2881 cgcaacttcc tgcaactctc tgcaactctc tgcaaacttt ttgagcatcg ttggtctagt
     2941 ctggtctaag atttcggctt gccatgtcga aggcatcggt tcaaatccga tacgatgctc
     3001 caaaaaaatt cccgcgggcg gaaacaattg accattccgg ttcttggctg gtctcgaagc
     3061 cgcgccgccc gccggaagga gcggatttga tgcaaataat ccgctcttaa ttctttatca
     3121 agcgttaaat caccgctgcc gaacgcttat tcacaactgt tgactgaaga ttcacaactg
     3181 ttgactgaag attcacacgc gttgagcgaa cattcacaac tgtcgaatga agattcacag
     3241 atgacgaatg aagattcaca actgttgaat gaacattcac aactgttgaa tgaacattca
     3301 caggtgacga atgaagattc acacgtgatg aataaagatt cacacgtgtt tgaccgaaat
     3361 tcgcccgggt aaacggctta cgggctcgac gagtttatca gaaccggaaa aattttccgg
     3421 tttaacaaaa aattcatata caaatcggat aaaaatcatc taaaatagcg ccagaagatt
     3481 tctgtcatac aaaatgttgt ggtgatgatt cgatttgttc cgacaatccg agttttaaaa
     3541 cactataata taaaataaaa aggaggcgat ttcatttgct gacaggaaga gttttactat
     3601 tgaatttctc atacgagccg ctcggaacgg tcggcattgc gcggtcaatg tgtctgtggt
     3661 ttcgcggctc cgtaacggtt gaagaatacg acggcgacaa tgttctgcat tcgccgcgcg
     3721 agacgtttcg cgtcccgtcg gtcgtgcgcc tgcgtcatta cgtcaacgtg catcgccgtc
     3781 gccgcgaaac gacgatgaaa cgggcgcgga tttatattcg cgaccgctat cgatgccagt
     3841 attgcggcga ccacaaacac gcggcggatt tgacgctcga ccacattctg ccgcgcgcgc
     3901 agggcggcga atcgacgccg cagaatctgg tcacggcttg cgtcaagtgc aacggtcgca
     3961 aaggcaaccg cacgcccgaa caagccagaa tgccgctgct tacttcacaa aagcttctca
     4021 atctgggatt agaccgcgtt ttgctttgcc actacgccga gtcaaaaccg gaatggaaga
     4081 aatatctctt tatggaagag tttgaagaac aaaccagagc agcttaagaa ttttagattt
     4141 tagattttag attgaaaacc aatcggcaat cgacaatcta aaattctttt cttatcggcg
     4201 cagcccgcgc catttttccc aaaactattt cagccgggaa ataacacgaa gcagcgaaaa
     4261 aatattttcg cgcggttttc gtgtgttttc aaggctgatt tttacagccg attagctcag
     4321 cggaacgaga gcggcggtct tctaaaccgc aggtcgtcgg ttcgagtccg acatcggcta
     4381 ttcttaaagg gtaaaggcta agggcgaaag gcgaaagatt aagtaagcaa acaattgccg
     4441 aattcattat tttttattca ttccttatcc tttatccttt cgccctcagc ctttaccctt
     4501 tagaaagttg cttcaattgg tcagagttcc caaaaggagc atttttttat gaaactcgtt
     4561 tttatttacg gctcgccggc ggtcggcaaa ctgacggtcg ccaatgaaat cgctcgaaaa
     4621 accgatttca aggtgtttca caatcacctg agcatcgatg caatcgagcc ggtttttgaa
     4681 ttcggctcgc cgtcgttcgg aaaattaatc gatttcatcc gcgtcgaaac cgtcaaagag
     4741 gcggcgcgcg tcgggctgaa cctgatttac acgttctgtt acgcgaaagg ctctgacgat
     4801 aaacacgtcg aaaaaatcgc gcgcgccgtc gaagaacacg gcggcgaagt ttgtttcgtc
     4861 cttttgaaag ccgaccgcgc agagctgaac aaaagagtcg tcgaagaatc gcgccgcgcg
     4921 atgggcaaag caaaaaccgt cgagatgatg gactttttcc acgaaaccta tgacttgctc
     4981 tcgccggttc ccgaccgcga aagcttaatc atcgacaata ctcatctttc atccgaaaat
     5041 gcggcggaac gcattatcga gcattttaat ttgcagcagc agcaaaaaat tatttgattt
     5101 gcttgcctga aaatacgaaa tcgagttaaa ataatttttc tgaaaataga cggaaatgat
     5161 tcaggcaagc gcctttttca tttccgtcta ttttttattt tgaaacatct accggagaat
     5221 ttatgagtaa aaaatacgaa accgaagacg aaattttagc cctggtcgaa tcattcgaaa
     5281 acggcgcgat tgcgcgcgaa gcctggaatc acgccggaca tttgacggtc gcgctttatt
     5341 actgcgcaca tcacgatttg gcagcggcga ctgaaaaaat gcgtcgcaat ctgttgcatc
     5401 atctgaaagc cgtcggcgtc gatttgacga aagaaatgcc gtatcacgaa accttgaccg
     5461 cgttttggat gcggacggtg aatgatttcc gaacatcgaa aaacggcgcg ccgcttctcg
     5521 tgacggcaaa cgagctaatc gccgggttcg acaaagatta tccgctccga ttttacagcc
     5581 gcgagctttt attcagcgac gaagcgcgcg cacgttttgt cgaagcggat ttgcccgaaa
     5641 ataatctctc gaatcaaaac tgattttaaa ccttttcggt taaatgcccg gcgaaaatcg
     5701 cccgttttaa attagtcgtc cgaaaagcct tattcgctcg aattcgccgg ttcttccgtt
     5761 tgataaacgc tgaaatcagt ccagagacgc cactcgtcca gatacggcgt cgggtcgatt
     5821 aaatcttttc gtcgctggta attgagcggt tcgacataca ggtaattcat attatgctcc
     5881 tgcgaaagcg gaccggaagc cgttccctta tacatttcta aatgcagcat tcctctgtcc
     5941 ttattatttt ttccgaccgt gccgatttgc tgtccttcgt atacttcttc gccgacggcg
     6001 acgggacgat tctcgtcgag ctcggtgtag cggataatga aattattgtg ccggatgacg
     6061 accgtgtaaa cgctttgata gaaaggcttc ttgggcactt ccagaactac gccgcgctcg
     6121 accgcgtaga ccggcgttcc cggcggagca atcaaatcgc aagccgcgtg gcggcgtgtg
     6181 cctcgatttt tcgtttgcgc gccgaaggcg atgccggagt aatgataatc agcctgggga
     6241 ataataggta atggaaatat agccatattt ttttctcctg aaattttatc ggcggcttgc
     6301 cgccttgcat atataaatgc gtccgaaatc tcaaaaaact atcacgcccg caaaaaaatt
     6361 ttttgttttt ttcgaggtgt gttaaattta aagtcgataa agtctgttat tgcgtaattt
     6421 aaaatgtcag ttccgaacac acaagaagtc accgaactgc tccagaaatg gagcgcgggc
     6481 gacgccggcg cgctcgacga gctgacgccc gttatttact cggagctgca ccgcatcgcc
     6541 cggcgctata tgagccgcga acgcgagaat cacacgctgc aaacgacggc gctggtcaac
     6601 gaggcttata tgcgcctgat tgattggaaa acggcgaaat gggaaaatcg agcgcatttt
     6661 ttcggcgttt ccgctcagct gatgcggcga attctggtgg atttcgcgcg cagccgcccg
     6721 aaagtcaagg acgaagtcgt gcatcacgtc tcgctcgaag aagcgtttgt cgtttccgcc
     6781 gaaaaggatg cggatttggt cgcgctggac gaagcgttga acgagctggc aaaattcgac
     6841 gagcgcaaag cgaagattgt cgagctgaaa tttttcggcg ggctgagcgt cgaagaaacg
     6901 gcggaagttt tgcggattgc gccgataacc gtaatgcgcg agtggcagaa ggcgaaagcg
     6961 tggctttacc gcgagctaag caaaaaataa aaattaatgc gaaggcgcga aaaaccgcga
     7021 attgcacgaa gatttttgtt tcgatacgac tcttttcact ttttcctgat ttcccattgc
     7081 ctttctccct gcacctttag catctttgcg ttaaaataaa attgtgaata cgacccgctg
     7141 gaaacaaatc gacgagcttt tcgacgccgt tctggacttg cccgaaacgg agcgcgaaaa
     7201 atttctgtcc gaaagatgca gcggcgacga agaattgcgg cgcgagcttc tgagcctgct
     7261 cgacgcccag aaagaaagcg aaaaaaactt tctgcaaaaa tcggcgatga acatcgccgc
     7321 caaaaatctc gccgaagacc aggcgattca tttcgacgcg cattttctca ataaaacaat
     7381 cggaacttat aaaatcgaaa gcctgctcgg cgccggcggc atgggcgaag tttatctggc
     7441 ggacgacgcc aagctgcgcc gcaaagtcgc gctgaaaatc ctgccttccg aatataatac
     7501 gagcgacgag cgggtaaagc gtttcgagct cgaagcccgc gccgtctcag ccctgaatca
     7561 tccgaacatc gtcaccatct acgacgtcgg aagctttgaa ggcatcaatt acatcgcgac
     7621 ggaatttgtc gaaggcaaaa cgctgcgcga ggttatcggc agccatctaa aattaaaaga
     7681 aattttatca ataatcattc aagcctgcga cgcgctcgcc gccgcgcaca gcgccggagt
     7741 cattcaccgc gacatcaagc cggaaaatat aatggtgcga cccgacggct atgtgaaaat
     7801 tctcgatttc ggtctggcaa aattgagcga gattgatttg cacacgctgc gcgattttgc
     7861 acagacggcg cagggcgtga ttatcggcac gccagcctat atgtcgcccg aacaggtcag
     7921 cgacgaaaat ctcgaccacc gcaccgattt gtggagcatc ggcgtcgttt tgtatgaatt
     7981 gttaacgggc gtcaatccct ttaaaaaaga aaatcgccag gcgacgtttc aggcgatttt
     8041 gtcgagcgac ccgccgcccg ccagttctct gaacgcggaa atctccgccg agctggaccg
     8101 gattttgacg aaagctctcg aaaaagacgc cgaattgagc taccagaccg cttcggattt
     8161 tcgcgccgat ttaaaacgcg tcaagcgcga aatcgattcg tcgccctcgt cgtggagcgt
     8221 cagaagcgcc gaagccgaaa cgctgcgccg ccggaaagac gccgccgaga ctcgtaaaaa
     8281 ttatctggtt tatgttctcg gcgcgcttgt tttgacggca atcggcatcg gcgtctggct
     8341 tttcgtcaaa aatgggcgcg cgccgcaaaa ccgcgaggcg agcgaatggg caaatgccag
     8401 acacgcgcag attaccgatt cgccctgggt cgaaggctat ccgagccttt cgccggacgg
     8461 gaaaaacatc atcttcgcca gcgaagccaa caacgacaga aatatttacc tgcaaagagt
     8521 cgg
//
LOCUS       DASWNE010000177         2176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_429562, whole genome shotgun sequence.
ACCESSION   DASWNE010000177 DASWNE010000000
VERSION     DASWNE010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429562"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..>2176)
                     /locus_tag="VGB68_03765"
     CDS             complement(101..>2176)
                     /locus_tag="VGB68_03765"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0048376.1"
                     /translation="LANNVNYIFRNHNFKFGGDFKRHRYDTNLPEEQATEFEKFDSFT
                     QLLTGNATEADTQYGVTDKSFRFMDFGFYLTDDWKISRKLTLNVGARYEIFMRPTEKN
                     GRIGNFDLSSFEPCFTQTGGTAEICNNPTRGFIIPSNAQLTGNPVVDGAISSTNVVGN
                     KHTLNGQDLNNLAPRIGLAYAVNDKLVFRGGYGLFYDRPSAAFINTIFSNYPFLREVE
                     ITVPSGNVPIATAFSNTPTNIPLNQWLPFRITRTAGAGGNYQIRDNTPITVDSRNTPF
                     GGTTFGNIAETFEFRAIDRDIRTPYVHQWNVGMQYEIFKDLMIEARYVGTAGRNLLMA
                     TALNQGFDLNDPQTPDHIYERFNQAYLAAGSPNGALNAGGSARERGLGRAFGFLNPVT
                     GRVDLNLANAAGAVINFEARVPILGFNVPEALMLRSNGYSDYHGAQFSLTKRLSHGLQ
                     FNVAYTFSKSIDIMSSDPGSTAGGGKPDVPNTGFIAQGDARNVNNNRGLSDFDRTHRF
                     STNFVYEIPSFGSANRFLTGWSISGFFQAQSGTPFTIYAAEPEIQNASQYADLTRGSG
                     GLYRLGFGRPNFVCSSASDAVLGYSGNDTLAIRRDCFGSPNGGFGNLGRNTFRGPAQK
                     RFDLSFVKNTKLTERFSLEVGFDLFNVFNTLNFANPNNDLQDTVDFGSITGTVGGPRV
                     GQFRAKFRF"
BASE COUNT          482 a          579 c          629 g          486 t
ORIGIN      
        1 ttacgaatta aaagaaattt tccttcattc gtaattcgta atttgtaatt cgtaattgaa
       61 aaagggcgag cttgcacggc ggcaatctcg ccctttttcg ttagaagcgg aattttgcgc
      121 ggaattgtcc gacgcgcggt ccgccgaccg tgccggtaat gctgccgaaa tcgaccgtat
      181 cctgcaaatc attgttcggg ttggcaaagt tgagcgtgtt gaagacgttg aaaaggtcga
      241 agccgacttc gagactgaac ctttccgtca gtttcgtgtt cttcacaaag ctgaggtcga
      301 aacgcttttg cgcaggtccg cgaaatgtgt ttctgcccag attgccgaaa ccgccgttcg
      361 gcgagccgaa gcaatcgcgc cggatagcga gcgtatcatt gccgctgtaa ccgagaacgg
      421 catcgctcgc cgacgagcag acgaagttcg ggcgaccgaa gccgagccga tacaaaccgc
      481 ccgaaccgcg cgttaaatcc gcgtattgcg aggcgttttg aatttccggc tcggcggcgt
      541 aaatcgtaaa cggcgtgccc gactgcgcct ggaaaaagcc ggaaatcgac cagccggtca
      601 gaaagcgatt ggcagagccg aaagacggaa tttcataaac gaaattcgtg ctgaagcgat
      661 gcgtgcggtc aaagtccgaa agcccgcgat tgttattgac attgcgcgcg tcgccctgcg
      721 cgataaagcc ggtgttcggc acgtccggct tgccgccgcc agccgtgctg cccgggtctg
      781 acgacataat gtcgattgat ttcgagaaag tgtaggcgac attgaactgc aagccgtgcg
      841 acaggcgctt cgtcaggctg aactgcgcgc cgtgataatc cgagtaaccg ttcgagcgca
      901 gcatcaaggc ttccggcacg ttgaatccca agataggaac gcgcgcttca aagttgatga
      961 ccgctccggc ggcgtttgcc agattcaaat cgactctgcc cgtcaccgga tttaaaaagc
     1021 cgaaggctct gcccagaccg cgctcgcgcg ccgaaccgcc cgcgttcagc gcgccgttcg
     1081 gcgaaccggc ggcgagataa gcctggttaa agcgctcgta aatgtggtcg ggcgtctgcg
     1141 ggtcgttcaa atcgaagccc tgattgagcg ccgtcgccat cagaagattg cgtccagccg
     1201 tgccgacata acgcgcctcg atcatcaaat ccttgaatat ctcatactgc ataccgacat
     1261 tccattgatg gacatacggc gtccggatat cgcggtcaat cgcgcgaaac tcgaaggttt
     1321 cggcgatgtt tccgaaagtc gtgccgccga aaggcgtgtt gcgcgagtcg acggttatcg
     1381 gcgtgttgtc gcggatttga taatttccgc ccgcgcccgc cgtgcgcgta atgcggaacg
     1441 gcagccattg attgagcggt atattggtcg gcgtgttgga aaaagccgtc gcaatcggca
     1501 cgttgcccga cggcacggtg atttccacct cgcgcaaaaa gggatagttg ctgaaaatcg
     1561 tgttgataaa cgccgcgctc gggcggtcgt aaaaaagccc gtagccgccg cggaaaacga
     1621 gcttgtcgtt gacggcgtaa gccaagccga tgcgcggcgc gagattgttc aaatcctgac
     1681 cgttgagcgt gtgcttgttg ccgacgacgt ttgtgctgct gattgcgccg tcaaccaccg
     1741 ggttgccggt caattgcgcg ttgctcggaa taatgaatcc gcgcgtcggg ttattacaaa
     1801 tctccgccgt gccgccggtt tgcgtaaagc acggctcgaa cgaggacaaa tcgaaatttc
     1861 cgatgcgccc gtttttctcg gtcggtcgca taaaaatctc gtagcgcgcg ccgacgttta
     1921 aagtcaattt cctggaaatt ttccaatcgt cggtcaggta aaaaccgaaa tccataaagc
     1981 ggaagctctt gtcggtgacg ccgtattgcg tgtcggcttc ggtcgcgttg ccggtcagaa
     2041 gctgcgtgaa gctgtcgaat ttttcaaact cggtcgcctg ttcttccggc agattcgtgt
     2101 cgtatcgatg gcgcttgaaa tcgccgccga atttgaaatt gtgattgcgg aaaatgtagt
     2161 tgacgttatt ggcaag
//
LOCUS       DASWNE010000178         1525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_357869, whole genome shotgun sequence.
ACCESSION   DASWNE010000178 DASWNE010000000
VERSION     DASWNE010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357869"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            423..683
                     /locus_tag="VGB68_03770"
     CDS             423..683
                     /locus_tag="VGB68_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048377.1"
                     /translation="MTNLEKRPIPDGRMRIIRLLSEAQKAALRALKPDGLLILNFYEI
                     EITQPETWIEYWFDENTLTEDDLRALINKTLIAAAENNYPAP"
     gene            complement(844..>1525)
                     /locus_tag="VGB68_03775"
     CDS             complement(844..>1525)
                     /locus_tag="VGB68_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY0048378.1"
                     /translation="YPSGGAMSIGRRRQLLEWAASVDGLIVEDDYDSEFRYEGRPLAS
                     LQGLDRSGRVLYIGTFSKTIFPALRLGCLVVPKDLIEIFTAVRALNGSHSALIDQATL
                     AEFINEGHFVRHIRRMRRLYEQRQEILISEVEKQLKESVEIRKSVAGMHLIGWLNDGV
                     KDYSVAGKAAEFGVKVGAVSSHSPTVAARGGLILGYTAIDEKQIKKGVRQLAKAMENV
                     EKTGSEPV"
BASE COUNT          379 a          389 c          336 g          421 t
ORIGIN      
        1 aattatgaat tctcaattac ttcacaaaag ataccgaaca tcaatttaga atttacaatt
       61 tagaatttac aattttccct ttctttattt ttgtgcaaag ccatggttcg gcgacgatgt
      121 aattttgcgc cgccggtaaa aataaacttt ctcgcccagc cttttgatta ttaaaattat
      181 ccggcttttt cttttgcagt tcgttcgctg ccgaactaaa atcaaaacaa attcgcgatt
      241 aataaaaccc gtgtgctttg ttatgacttt ataactcaaa agtgcgagcg cggcgcggcg
      301 gttaattttg aaaaccgacg cctttttaat tgcggcgcaa aaaaaacgtc catatccgta
      361 tccggtattt cggtcattag tcagaccgaa agaatatcga gttattgtta tcatccgagc
      421 ttatgactaa tttggagaaa cgcccgattc ccgacggtcg aatgagaatt atacggcttt
      481 tgtccgaagc gcagaaagcg gctttaagag cgctcaagcc ggacggatta ctgattctca
      541 atttttacga aatcgaaatc acgcagcccg aaacctggat tgaatactgg tttgacgaaa
      601 atacgctgac cgaagacgat ttacgcgccc tgataaataa aactctcatc gccgccgcgg
      661 aaaataatta tccggctccg taaaatttat ggcaatctgt ccggttattt ttgcattttc
      721 gacatattta tcgaacccgg tcagcggctc atacaaaagg cgtccgattg atttttcagc
      781 ggtataattg cgcgcgtcaa gccgctgcgt aacgcggcgc gcggcaattc cgcgtcataa
      841 ttttcaaacc ggctcactgc ctgttttttc tacgttttcc attgctttgg ctaactgtct
      901 gacgcctttt ttaatttgct tttcatcgat tgccgtatag ccgaggatta aaccgccgcg
      961 cgcggcgacg gtcggcgagt gcgacgagac tgcgccgact tttactccga actcggcggc
     1021 ttttccggcg accgaataat ccttgacgcc gtcgttcagc cagccgatta aatgcatccc
     1081 ggcgaccgat tttctgattt cgacgctctc ttttaattgt ttctcaactt cggaaatcag
     1141 tatttcctgt ctttgctcat ataatctacg catccggcgg atgtggcgca cgaaatgtcc
     1201 ttcgttgata aattcggcaa gcgtggcttg gtcaatcaac gcggaatgcg agccgttcag
     1261 cgcgcgaaca gccgtaaaaa tctcgattaa atctttcggc acgacgaggc atccgagccg
     1321 cagcgccgga aaaatcgttt tactgaaagt gccgatatac agaacgcgcc cgctccggtc
     1381 gagtccctga agcgaggcga gcggtctgcc ttcgtagcgg aattcgctgt cgtaatcgtc
     1441 ctccacgatc aaaccgtcga cgcttgccgc ccattccagc aactgccgtc gccgaccgat
     1501 cgacatggct ccgcctgacg gatat
//
LOCUS       DASWNE010000179         6161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_216967, whole genome shotgun sequence.
ACCESSION   DASWNE010000179 DASWNE010000000
VERSION     DASWNE010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6161
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216967"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(302..598)
                     /locus_tag="VGB68_03780"
     CDS             complement(302..598)
                     /locus_tag="VGB68_03780"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEY0048379.1"
                     /translation="MTYIVVEPCVECKYTDCAAVCPVEAFHELSDRLLINPDTCIDCD
                     ACLPECPVEAIYSDMSIPPEYMDWLEKNAEAENYPIISTKQPALFGPGCKGNPE"
     gene            722..1762
                     /gene="glpX"
                     /locus_tag="VGB68_03785"
     CDS             722..1762
                     /gene="glpX"
                     /locus_tag="VGB68_03785"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEY0048380.1"
                     /translation="MKLKAERELSLDFLRVCEAAAIESAKTMGQGDRKYSDHVAVEAM
                     RQVMDTVPMRGRIVIGEGERDEAPMLYIGEELGGGNYAEEERANFPEVDIAVDPLEGT
                     NLCALGANNAIAVLAAAERGGLLNAPDIYMDKIVVGPSCKGAVDIDAPVKDNLKNIAR
                     RLGRDIDDLTVICLDRSRHKQLINDVRAAGARIRLISDGDLSAGISAAVAGTNIHALM
                     GIGGAPEGVITAAAMKCLNGEILAKLVFDPERLGVDKSKVPPAAEVMRRLKKMGITDP
                     DKIYDTNDLAPGKKIIFAATGVTDGALLRGVRFFGAGKRTHSVVMTSDSKSIRFVDTV
                     HVEGGPDAVIRF"
     gene            1850..2401
                     /locus_tag="VGB68_03790"
     CDS             1850..2401
                     /locus_tag="VGB68_03790"
                     /inference="COORDINATES: protein motif:HMM:NF021384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HEY0048381.1"
                     /translation="MPETIENYIPQALVKQQKRQAFFAWGLVFLIALVWIGLIVSAPL
                     ARANDFTAFSAAVYNFFGYLCHQIPERSFHASGFPFAVCSRCFGFYFGFLLGLGVYPF
                     FRALDVVEPFPRFWLFLAIVPMGIDWSLGFFGIWANTHVSRSLTGVILGFACGFFIVP
                     ALAEISRFFVDRKVKRLSRRQPF"
     gene            2782..3018
                     /locus_tag="VGB68_03795"
     CDS             2782..3018
                     /locus_tag="VGB68_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048382.1"
                     /translation="MAQRLGVAESEIQLASASDEDFPNSSLGAPVDDEMSAQMISSGW
                     KINLQADGKNYEYRADKYQLRLRGFKGTNYIIES"
     gene            3073..4512
                     /locus_tag="VGB68_03800"
     CDS             3073..4512
                     /locus_tag="VGB68_03800"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY0048383.1"
                     /translation="MNRQNIWIHITIAVIIITMAAVLGQIRRWQSALGMFNDKASVMK
                     PAKPTTVNRTTKDCQPEVLVKFRPEVGLAEIKKIAAKNNDKVEDEIEAVKNLISIDDL
                     DGLEAEKVAEQYRRMSDLVLYAEPIVQIKLEPTETFREITTKNSESPNLPGELNLPND
                     PMFGDQWALKNGGQNGGKENADIAAPKAWEKTKGSRKVVVAVLDTGVDYLHLDLAANM
                     WIRPDGVPQYKDDELGVFNDLNGFNASENLSDPMDENGHGTHCAGVVGAEGDNDLGIA
                     GINWQVEIMPLKFLGRGGFGSTKDAIEAINYAIDRKQKGVNVRVISASWGSTAYSRAL
                     EDAIRAAGEQGILFVAAAGNDSTNNDKSPHYPSNYKLPNVISVAALDRSDNLASFSNY
                     GATSVHIAAPGKDILSTWLKEGYREASGTSMATPQVSGVAALILANEPNLTVEKLRER
                     LLKSVDKIDSLNGKVENGGRLNAAKALGN"
     gene            4836..5384
                     /locus_tag="VGB68_03805"
     CDS             4836..5384
                     /locus_tag="VGB68_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048384.1"
                     /translation="MNRFKNLLAAFAFSLLILGLPALASAQWRDDDYNRNDRNGDYRN
                     NRNYNRGNLRSAINRLKNRAEVFERRLDRELDNSRYDNRNREDRLNRLADDFTNAAER
                     LEDAFDGGRNMENSYDEARRVLQLAQQIDNAMSRSRLGGYARNEWNAMRQDLNTIANA
                     YGNNRNRNRRGNNGDWRDYLPF"
     gene            5522..>6161
                     /gene="rsmI"
                     /locus_tag="VGB68_03810"
     CDS             5522..>6161
                     /gene="rsmI"
                     /locus_tag="VGB68_03810"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEY0048385.1"
                     /translation="MFGTLYLVATPIGNLQDITFRAVEILKTVELIACEDTRHTRKLL
                     THYGISNRLVSYHEHNEHERAEELAARLQDGKSIAVVADAGTPAICDPAFRIVQKALI
                     IGAPVVSIPGAAAFLSALIISGLPTDSMFFGGFLPSKKSERQKRLAEVREIPATLVFY
                     ETPHRLAKSLADCAEVLGDRKAVVVRELTKLHEETIRGNLIGLAKTFSEKTVK"
BASE COUNT         1578 a         1457 c         1630 g         1496 t
ORIGIN      
        1 gacattttat aaggattttt ccaagcgaaa tttgaaaaat gactttaact tcgtgaaaac
       61 cggtgggaat cgcatatttc agcgaacaat tcaatattat aacggataat tacaaatttt
      121 tgtgaaaaat cttgcagcgg ggcgattaat cggaaattat cgcgaaaatt cgcatatttt
      181 ggcggcgaat cgcatatttt gattaatagt tcggatattt tcaatcagtt atgacaaaaa
      241 gaaaaagtga acgacaaata aaagattcgt cgttcaccgg cgtttgaaaa atttgtaaaa
      301 cttattcggg gttgccttta cagcccggac cgaacagcgc gggttgtttg gtgctgataa
      361 tcggataatt ttccgcttcc gcgttttttt ccagccaatc catatattcg ggcggaatcg
      421 acatatccga ataaatcgcc tcgaccgggc attccggcag gcaggcgtcg caatcgatgc
      481 aggtgtcggg attaattaaa agccgatcgg aaagttcgtg aaaggcttcg accgggcaaa
      541 ccgccgcgca atcggtgtat ttacattcca cgcacggctc aactacgata tacgtcattt
      601 taaaaaaatc tcctcagcag aataataagt taaaagaaag attatacttg tcattaagcg
      661 atagacaagt gttaaaatgc cttcaatatt tattaccgag aaattttaag gaaaaccgag
      721 aatgaaatta aaagcagaac gcgaattatc attggatttt ctgcgcgtgt gtgaagccgc
      781 cgcgattgaa tccgccaaga cgatgggaca aggcgaccgc aaatattccg accacgtggc
      841 ggtcgaggcg atgcgacagg tgatggacac cgtgccgatg cgcgggcgca tcgtcatcgg
      901 cgagggcgag cgcgacgaag cgccgatgct ttatatcggc gaagagctcg gcggcggaaa
      961 ctacgccgaa gaagaacgcg cgaattttcc cgaagtcgac atcgccgtcg acccgctcga
     1021 aggcacgaat ttatgcgcgc tcggcgcaaa caacgcgatt gccgtcttag cggcggcgga
     1081 acgcggcgga cttttaaacg cgccggatat ttatatggac aaaatcgtcg tcggtccgtc
     1141 gtgcaaaggc gcggtcgata tcgacgcgcc ggtcaaagac aatctgaaaa atatcgcgcg
     1201 gcggctggga cgcgacatcg acgacttgac ggtgatttgt cttgaccgct cgcgccataa
     1261 acaattaatc aacgacgtcc gcgccgccgg cgcgcgaatt cgccttatca gcgacggcga
     1321 tttgtcggcg ggaatttcgg cggcggtggc gggcacaaat attcacgcgc tgatgggcat
     1381 cggcggcgcg ccggaaggcg tcatcacggc agcggcgatg aaatgtctga acggcgagat
     1441 tctggcaaag ctcgttttcg accccgaaag attgggcgtc gataaatcga aagttccgcc
     1501 cgccgccgaa gttatgcggc gtttgaaaaa aatgggaatc accgaccccg acaagattta
     1561 tgacacgaac gacctcgcgc cgggcaaaaa gattattttt gcggcgaccg gcgtcaccga
     1621 cggcgcgctg ctcagaggcg ttcgcttttt cggcgcgggc aagcgcacgc attcggtcgt
     1681 gatgacctca gactcaaaaa gcatccgttt cgtcgatacg gttcacgtcg aaggcggacc
     1741 tgacgcggtt attagatttt agaatttttc gccgcgaaac ggcacgaaaa gacgcgaaaa
     1801 gcttttatgt tttattttgc gttctggcgt gacctttcgc ggctaaatta tgcccgagac
     1861 gattgagaat tacattccgc aggcgcttgt caaacagcag aaacggcagg cttttttcgc
     1921 ctggggattg gtttttttga ttgcgctcgt ctggattggt ttaatcgttt ccgcgccgct
     1981 tgcccgggca aacgatttta cggctttttc cgccgccgtt tacaattttt tcggctatct
     2041 ctgtcaccag attccggagc gttcgtttca cgcgtccggt tttcctttcg ccgtctgctc
     2101 gcgctgtttc ggtttttatt tcggatttct gctcggtttg ggcgtttatc ctttttttcg
     2161 cgcgctggac gtggtcgagc cgtttccgcg tttctggctt tttctggcga tagttccgat
     2221 gggcatcgac tggtcgctcg ggtttttcgg gatttgggcg aacacgcacg tttcgcgctc
     2281 gctgacgggc gtgattctgg gttttgcctg cgggtttttc atcgttccgg cgctggcgga
     2341 aatcagccgg ttttttgtcg accgaaaagt aaaaaggctg tctcgccgac agccttttta
     2401 gcatctaaat ttatcttgga taagtagttt ttaaactgtg ttggcaacgc cgacgccggc
     2461 gttccgtgat aagcaatact tattatattc gttttccggt caaattgaca agttttgttt
     2521 ttagaaaact cgcgggcgga ataagatatt tttaccgcaa aggcacagac ccgcgcggaa
     2581 aaacaaggca aatgtagaaa ttgaaaggca aaaataaaag actgtttcgc atttttgcca
     2641 tttgcctttc agcttgcgtc ttattttccg attgactatt ttgccgaata gtcgcaaaat
     2701 tacctttgct ttcatttaaa taaggagtgc taacaggtga gttttagccg tgatagtgca
     2761 gttcaaagag cgaaacagga cttggcacaa cgtttaggag ttgcggaatc cgagattcag
     2821 cttgcttctg caagcgacga ggattttccg aattcctctc ttggcgcgcc ggttgacgac
     2881 gaaatgtctg cgcagatgat ttcgagcggc tggaagataa atttgcaagc cgacggaaaa
     2941 aattatgaat atcgtgccga taaatatcag ctcagattgc gcggatttaa gggaacaaat
     3001 tacattatag aatcgtgaaa taagcggaaa ggctcgcggg tctttccgct taaagtattt
     3061 tgggagtaaa caatgaatcg acagaatatt tggattcata tcaccatcgc cgtaattatc
     3121 attacaatgg cggcggttct cggacaaatc aggcgttggc agtcagcgct cggaatgttt
     3181 aacgacaagg cgtccgtgat gaagccggcg aaaccgacga ccgttaatcg cacgacgaag
     3241 gattgccagc cggaagttct ggtgaagttt cgtcctgaag tcggtttggc ggaaattaaa
     3301 aagattgccg ccaaaaataa cgacaaggtc gaagacgaaa tcgaagcggt caaaaacctg
     3361 atttccatcg acgatctgga cgggctggag gcggaaaaag tcgccgagca gtatcgacgg
     3421 atgtcggatt tggttttgta tgccgagccg attgtgcaaa tcaagcttga gccgaccgaa
     3481 acttttcgag aaattactac gaaaaattcc gaatcgccga atttgccggg cgagctgaat
     3541 ttgccgaacg acccgatgtt cggcgaccag tgggctttga aaaacggcgg tcagaacggc
     3601 ggcaaggaaa acgccgacat cgccgcgccg aaagcgtggg agaaaaccaa aggcagccga
     3661 aaagtcgtcg tcgccgtgct ggacacgggc gtcgattatc tgcatctgga tttagcggcg
     3721 aatatgtgga ttcgtcccga cggcgttccg caatataaag acgatgagct tggcgtcttc
     3781 aacgatttga acggttttaa cgcgtcggaa aatctgtccg acccgatgga cgaaaacggg
     3841 cacggaacgc attgcgcggg cgtcgtcggc gcggaaggcg ataacgattt gggaatcgcc
     3901 ggaatcaact ggcaggtcga gattatgccg ctcaaatttt tgggacgcgg cggtttcggc
     3961 tcgaccaaag atgcgattga agccatcaat tacgcgattg accgcaagca gaaaggcgtc
     4021 aacgttcgcg tcatcagcgc cagctggggc tcgaccgctt attccagagc tctggaagac
     4081 gcgattcgcg cggcgggcga gcagggaatt ttatttgttg cggcggcggg aaatgattcg
     4141 accaataacg ataagagccc gcattatcct tcaaactaca agctgccgaa cgttatcagc
     4201 gtagcggcgc tcgaccgctc ggacaatctc gcttcgtttt caaactacgg cgcgacatcg
     4261 gttcatatcg ccgcgcccgg caaagacatt ctttcgacct ggctgaaaga aggttatcgc
     4321 gaggcttcgg gaacttcgat ggcgacgccg caggtttccg gcgtggcggc gctgattctg
     4381 gcaaacgagc cgaatctgac ggttgaaaag ctccgcgaaa gactcctgaa atcggtggat
     4441 aagattgatt ccttaaacgg aaaagtcgaa aacggcggca ggctcaacgc ggcgaaagcg
     4501 ctcggaaatt aaacttaatc aatcaggaaa aactttgctg cggtgagaaa tttctcgccg
     4561 catttttatt ttaaccaatt cgtcagatgc tcaaccattt ggaacatagc ggcggacgcc
     4621 ggagttttaa aaaaaccttc aaataaattc ctttatttct tcaaataact gaaaacacag
     4681 tatttgctta atctaatcgc ctttcgaaag ataatggcac acactttgct tttaccttcg
     4741 cggcaacgca gcgcatcacg aaaagcgact ggttgcggtt tgttgaaagg aaactttttt
     4801 tgttggtaaa gaaacttttg aggagaaaaa tcataatgaa tcgttttaaa aatttacttg
     4861 cagcttttgc gttctctctg ttgattctgg gcttgccggc gctggcttcg gcgcaatggc
     4921 gcgacgacga ttataatcgc aacgaccgaa acggcgatta tcgcaacaat cgaaattaca
     4981 atcgcggcaa tctgcgctcg gcgattaatc gcctgaaaaa tcgagctgaa gtttttgagc
     5041 gacggctcga ccgcgagctt gataacagcc gttacgataa ccgcaatcgc gaagaccgtc
     5101 ttaatcgttt agccgatgat tttaccaatg ccgccgagcg gctcgaagac gcattcgacg
     5161 gcggacgtaa tatggaaaac agctacgacg aagcgcgccg cgttctgcag ctcgcgcagc
     5221 aaatcgacaa tgcgatgtcc cggtcgcgtc tgggcggcta cgcgcgcaac gaatggaacg
     5281 cgatgcgcca ggatttgaac acgattgcca atgcctacgg caacaatcgc aatcgtaatc
     5341 gacgcggcaa caacggagat tggagagatt atctgccttt ctaaaattta taaaaggttt
     5401 aacgcggctt cgctttccag accttccgaa tccagaatgg agtcgtgtta atcgaaaagc
     5461 ccgagtttaa agactcgggc ttttttgcgt ttagtgtatc gttcgttgta tttttctttc
     5521 tatgttcgga acactttatc tggtcgccac gccgattggc aatttgcagg acatcacgtt
     5581 tcgcgccgtc gaaatcttaa aaaccgtcga gcttatcgcc tgcgaagaca cgcgtcacac
     5641 gcgcaagctt ttgacgcact acggcatttc aaaccggctc gtcagctatc acgaacacaa
     5701 cgagcacgag cgcgccgaag agttggcagc ccgtctgcag gacggaaaat caatcgccgt
     5761 cgtagcggac gccggaacgc ccgcaatctg cgacccggcg tttcggattg tgcaaaaggc
     5821 tttgataatc ggcgcgccgg tcgtttcgat tccgggcgcg gcggcttttc tgagcgctct
     5881 gattatttcc ggtttgccga ccgattcgat gtttttcggc ggatttctgc cttccaaaaa
     5941 aagcgagaga caaaagcgtc tggcggaagt cagggaaatt cccgcaaccc tggtttttta
     6001 cgaaacgccg caccgcctgg caaaatcgct ggcggactgc gccgaagttt taggcgaccg
     6061 aaaagccgtc gtcgtccgcg aattaaccaa attacacgaa gaaacaattc gtggaaattt
     6121 aatcggactg gcgaagactt tttcggaaaa aacggtcaaa g
//
LOCUS       DASWNE010000180         1995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_641623, whole genome shotgun sequence.
ACCESSION   DASWNE010000180 DASWNE010000000
VERSION     DASWNE010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641623"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..1921)
                     /locus_tag="VGB68_03815"
     CDS             complement(131..1921)
                     /locus_tag="VGB68_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048386.1"
                     /translation="MKLPASLAPWARQLKIFPDEISLALGALAQKISPFVTPLRSQEE
                     ISDREPNGYDGVARRGIYERLLLSELALADEISEEFVRRAVMGEHLFLNLARVAPSAK
                     RMSIALFSAGPEQLGTPRIAHLAALIVLARRAESVKAAFEWGVLQDEELKTFSEVTES
                     NVLSLINARTAAALKPGHIAKWREKLQELETVADVWLLGDNNLKDFKEAKGFSLLTVE
                     DVLEPGARRLELSVISSSGTQKQTTLELPDEAICTRLLRNPFEVYKAPEKVNLTTKLS
                     SFFFDENGAKIFGRGDDKTVFYLPVANSPNAVAGSPRRYRLYHQNDLLLAAGRLGKSV
                     ATIAFTEAGHLRLDYPKSGNFRLPQGNYLLGKAQLILPDDNKTLLPVYNLRFPDSPFF
                     QAAFTDAKNNLFILNRADNIGEELVGRALLNATGVLAATRTDRRFVFVGCEPENKILR
                     IISYGEQTERRDIPLAPVTQAFFGRGIGVSHPTFGLLALGDEKNHFVIIKNGEEKYID
                     ATPTGEVVGVFYDAGFSPEVGLFELEEDRRTLNFNVGRKHNKQVFISGEEIEKIVFSH
                     RSPVCAYQTISGKIIIFSLTHRVPIGSFGS"
BASE COUNT          513 a          503 c          479 g          500 t
ORIGIN      
        1 ctgccagaat gccaggatac gccggcgcat ttccgcttcg ctcagaattt tcgccgaaag
       61 caaaaaagct tccgccgaaa cggttccgcg atgcgtcaga ttttgaggag taataatttt
      121 catttcctta ttaactgccg aaacttccga tgggcacgcg gtgcgtcagc gaaaaaatta
      181 tgatctttcc tgaaatcgtt tgataagcgc agacggggct gcgatgactg aaaacgattt
      241 tctctatttc ctcgccggat ataaaaacct gtttgttgtg ttttctgccg acgttaaaat
      301 ttaaagttcg gcggtcttct tcaagttcaa acaacccgac ctcaggtgaa aaaccggcgt
      361 cgtaaaaaac tcctactact tcgccggtcg gcgtcgcgtc gatatatttt tcttcgccat
      421 tttttataat gacaaaatga tttttctcat cgcccaaagc aagtaatccg aaggtcggat
      481 gcgacacacc gatgcctcta ccgaaaaatg cctgcgtaac aggagccaaa gggatgtctc
      541 ttcgctcggt ctgctctcca taagagatta tccgcaaaat cttattttcc ggttcgcagc
      601 cgacaaatac aaaacgccgg tcggtccgag ttgccgccag cacacccgtc gcgttcaaaa
      661 gcgcgcgtcc taccagttcc tcgccgatat tatctgctct attaagaata aaaagattat
      721 ttttcgcatc tgtgaaagcg gcttggaaaa acggagaatc cggaaagcgc agattgtaaa
      781 ccggcagcaa agttttatta tcgtccggca aaatcaattg cgcttttcca agcagataat
      841 tgccttgagg caggcgaaaa ttgccggatt taggataatc gagccgcaaa tgtcccgcct
      901 cggtgaaagc aatagtggcg acggatttcc cgaggcgtcc ggcagccagc agcaaatcat
      961 tctggtgata cagtcgatac cggcgcgggc ttccggcgac ggcattcgga gagttggcaa
     1021 caggcaaata aaacaccgtt ttgtcgtcgc cgcgcccaaa gattttcgct ccgttttcat
     1081 caaaaaagaa actactgagc tttgtagtaa gatttacttt ttccggcgct ttgtaaactt
     1141 caaaaggatt tcgcagcaga cgcgtgcaaa tcgcttcgtc gggaagctcg agcgttgttt
     1201 gtttttgggt gccggaagaa gatataacgg ataattccaa tcgcctcgcg cccggctcca
     1261 aaacatcttc gaccgtgagc agcgaaaatc cttttgcttc tttgaaatct tttaaattat
     1321 tgtcgcctaa gagccaaaca tcggcgacag tttccaattc ctgaagtttt tcgcgccatt
     1381 ttgcgatatg cccgggttta agagccgcag ccgtgcgcgc gttaatcaaa cttaaaacat
     1441 tcgattcggt aacttccgaa aacgttttta gctcttcgtc ctgcaaaacg ccccattcga
     1501 aagcggcttt gactgattcc gcacggcgcg ccaggacaat caaagcggcg agatgagcga
     1561 ttcgaggcgt tccgagctgt tccggtcccg cactgaaaag tgcgattgac attcgttttg
     1621 ccgaaggagc cacgcgagcg agatttaaaa atagatgttc gcccataacg gcgcggcgga
     1681 cgaattcctc tgatatttcg tcggctaaag ctaactcgga aagtaaaagc cgctcgtaga
     1741 ttccgcgccg cgcaacaccg tcataaccgt taggttcgcg gtcggaaatt tcctcctgag
     1801 aacgcagcgg agtgacaaac ggcgaaattt tttgcgccag agcgccgagc gcgaggctga
     1861 tttcatcggg aaagatttta agttgccgcg cccagggcgc gagactagcc ggtaacttca
     1921 tcgattttca gaaagccagt tttccactgc ggctcgcgat aagggtttag ccgcgccgaa
     1981 agggataatt ttttc
//
LOCUS       DASWNE010000181         2888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_288139, whole genome shotgun sequence.
ACCESSION   DASWNE010000181 DASWNE010000000
VERSION     DASWNE010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288139"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..2045)
                     /locus_tag="VGB68_03820"
     CDS             complement(126..2045)
                     /locus_tag="VGB68_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048387.1"
                     /translation="MKQKKTLQTGKRDRTGERGAALVMVLMIAFLLLVASAALLLESS
                     MNTANVTDAVAEQQAYYAAESGIQSALDVLRGNTTVIEPIASLNEPPPPNANGEDWAW
                     INPLQWIETRASAAPSPTPTLDATLNQIDFRKAVNIATSNKSNDNSTTPRLSRWLNYN
                     YTPTGQAIADRVTMGTGTYNPDSGMAYSVTVTDPDNIGNTVSYSTIGRIVKPDGTVTS
                     SYTIMSGSNSATISYIPVIPSTKTVTINSGSIDTNLGYFRISYGGDSNGVTLSESLRF
                     EIAVNMVSPYQSTRLIRGLILPSGVIKSESPGVTFLYDSQIFNLMGSWITLQNVNFVN
                     LPNNTPPKIGYEVIPNAPNNSFGFTAVNASMTPPEPTRVVVRAVGYGPRGARKQLETV
                     VQKNFFNGLSAPATLTLVGPKTKTGYPNFVFEPGNSQNVTYSGDDVVSNMLIPSIGTT
                     NNDSLDTVEGVVLCGKHCKSNIAGFPANVTDEMPPWLQSAKNLNDTLEALKIVAKASG
                     RYYAAGQTPPNFGDNVNARGITFVDGDVSLSGAGGGILVCTGKLTLNGGFDFNGLIIA
                     TGTQGVRRSGTGNGEIHGNMVVAPYDKNNITAGFVPPQFDTRGGGNSEMRYNSSSVAN
                     GMLAVSNFVQGVAEK"
     gene            complement(2106..2750)
                     /locus_tag="VGB68_03825"
     CDS             complement(2106..2750)
                     /locus_tag="VGB68_03825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY0048388.1"
                     /translation="MKIAHRQKQKGFSLLELIIAMTITLILMAIAATLFSGALGTRAR
                     ESRRTDALTSAQAAITVMSREIANSGYGLTNNGIVYVDSGAQRLHFRSNVTNSNTVTC
                     DPGEDVTYFYDAASSSIVRYDRFPSTPTNCGTATGETSVVVNRISNVTFQYFDYTGSN
                     STPTVSATPTINTSRVRITVTVQLDQVQGQPNNQTVTFTSDVTLRNSDYMLNQY"
BASE COUNT          641 a          748 c          781 g          718 t
ORIGIN      
        1 aactacaagc attaataaac acagtctgaa attcatatta ttttaatctc gaatattgaa
       61 gaaaaaaaca aaggaaaaag gtgcgggagt gaggcttttt ccttttgccg gagtatattt
      121 tacgtttatt tttcagccac gccctggacg aaattgctga cggcgagcat cccgtttgcg
      181 acgctgctgg agttgtagcg catttcggaa ttaccgccgc cgcgcgtatc gaattgcggc
      241 ggcacaaagc cggcggtgat gttgtttttg tcatacggcg caacgaccat atttccgtga
      301 atttcgccgt ttccggtgcc gctgcgccgc acgccctgcg tgccggtcgc gattatcaac
      361 ccgttaaagt cgaaaccgcc gttcagcgtc agcttgccgg tgcaaaccag aattccgccg
      421 cccgcgccgc tcaaactgac gtcgccgtct acgaaagtga ttcctctggc gttgacgtta
      481 tcgccgaagt tgggcggagt ttgtcccgcc gcgtaatagc gacccgacgc tttggcgacg
      541 attttcaggg cttcgagcgt gtcgttcaga tttttggcgc tttgcagcca gggcggcatt
      601 tcatcggtca cattggcggg aaaaccggcg atgttcgatt tgcagtgctt gccgcacaaa
      661 accacgcctt cgacagtatc caaactgtca ttgttggtgg tgccgataga tggaatcagc
      721 atatttgaaa cgacgtcgtc gcccgaataa gtaacgtttt gcgagttgcc cggttcgaat
      781 acgaaattcg ggtagccggt tttcgttttc ggtccgacga gcgtcaaagt cgcgggcgcg
      841 gataaaccgt taaagaaatt cttctgaacg accgtttcca gttgtttgcg cgctccgcgc
      901 ggaccgtagc cgacggcgcg aacgacgacc cgggtcggct cgggcggcgt catcgaagca
      961 ttgacggcgg taaaaccgaa cgaattgttc ggcgcattcg gtataacctc ataaccgatt
     1021 ttcggcggcg tattattcgg caaattaacg aaattgacgt tttggagcgt aatccagctt
     1081 cccatcaaat taaatatctg cgaatcgtat aaaaaagtta cgcccggaga ctcgctttta
     1141 attactccgc tcggcaatat caagccgcga atgagtcggg ttgactgata gggcgagacc
     1201 atattgacgg caatttcaaa acggaggctt tccgaaagag tgacgccatt ggaatcgccg
     1261 ccgtaagata ttcgaaaata accgagattc gtatcaatcg agccggaatt aatcgttacg
     1321 gttttcgtcg aaggaatgac gggaatgtag gaaatagttg ccgaattaga ccccgacatt
     1381 attgtgtatg agctggtaac cgtgccgtcc ggcttcacga tgcgaccgat tgtgctatac
     1441 gaaaccgtat tgcctatgtt gtcggggtcg gtcacggtta cgctgtatgc cattccgctg
     1501 tcgggattgt aagtgcccgt gcccatcgtc acgcggtcgg cgatggcttg tccggtcggc
     1561 gtgtaattgt aattgagcca gcgcgacagg cgcggcgtcg tgctgttatc gttcgattta
     1621 tttgaagtgg caatattgac cgccttgcga aaatcgattt ggttgagggt cgcatccagc
     1681 gtcggcgtcg gcgacggcgc ggcgctcgcc ctggtttcaa tccactgcag aggatttatc
     1741 caagcccaat cttcgccgtt cgcgtttggc ggcggcggct cgttaagcga agcaatcggc
     1801 tcgattaccg tcgtattacc gcgcagaacg tcgagcgccg attgaatgcc gctctccgcc
     1861 gcgtaataag cctgctgctc ggcgacggcg tcggtgacgt tcgccgtatt catagacgat
     1921 tccagcagaa gcgccgcgct cgccacgagc agcaaaaacg caatcattaa aaccatgacc
     1981 aatgccgcgc cgcgctcgcc cgtgcggtcg cgttttccgg tctgcaatgt ttttttctgt
     2041 ttcataacgg ctctcctttg cttcaaataa agactttata tcaaaaattt tccgacccga
     2101 aaaaatcaat attgattcaa catataatcg gaatttcgca gcgttacgtc cgacgtaaac
     2161 gtaacggtct ggttattcgg ctgaccttga acctggtcca gctggacggt gaccgtaatc
     2221 ctgacgcgac tcgtattaat cgtcggagtc gccgagaccg tcggcgtcga attacttccc
     2281 gtgtaatcaa aatattgaaa tgtcacgttg ctgattcgat taacgaccac ggaagtttcg
     2341 cccgtcgccg tgccgcaatt ggtcggcgtg gacggaaagc ggtcgtatct gacaatcgag
     2401 ctggaagcgg cgtcgtaaaa atacgtcacg tcttcgcccg ggtcgcaggt gacggtgttc
     2461 gaattcgtga catttgagcg gaaatgcaag cgctgcgcgc ccgaatcgac atagacgatt
     2521 ccgttgttgg tcaatccgta gccggaattg gcaatttcgc gcgacatcac ggttatggcg
     2581 gcttgcgccg aggtgagcgc gtcggtgcgc cggctttctc tggcacgagt tccgagcgcg
     2641 ccgctgaaca aagtagcggc aatcgccatc agtatcagag ttatagtcat ggcgattatc
     2701 aattccagca gcgaaaagcc tttctgtttt tgccggtggg caattttcat ttttaacact
     2761 ttcctttagt ttgacctgac gcgccgcaaa aaaaccgtcg cgggaacagc cgtctgccag
     2821 cccggagtcg ggctctcgct cgtcaccctg acggttattt cctttaaagt tctggcggca
     2881 tcgacgtc
//
LOCUS       DASWNE010000182         2456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_288179, whole genome shotgun sequence.
ACCESSION   DASWNE010000182 DASWNE010000000
VERSION     DASWNE010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288179"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..>2456)
                     /gene="mfd"
                     /locus_tag="VGB68_03830"
     CDS             complement(27..>2456)
                     /gene="mfd"
                     /locus_tag="VGB68_03830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEY0048389.1"
                     /translation="KFEFETIGNLRLKTEDKKPLFLFSTAEKPVEFEIQSRSTRKFHG
                     NLKDFANEIKSPAKASENQIIVLQSQGLAERLTEILRDYDAHLPGSALLVGDLSNGFE
                     IPSLDLIVQTETDIFGETTQQEFQRPKIKDQRPKTKLGAFISDFRDLKPGDYVVHVDH
                     GIGRFEGLQIIESQGIQREFMLLIYADNTKLFVPVERLDLVSRYSSGEATQPSLDRLG
                     GIGWQKTKAKAKRAMRDMADELLRLYAERKLVQGFAFSADSPWQEEFEDAFPFELTPD
                     QSTTIEAVKSDMETLTPMDRLIVGDVGYGKTEVAMRAAFKAVMDSKQVAILAPTTVLA
                     YQHFESFKKRFAAFPVSMELLSRFRSPKEQKAVVEKAEKGELDILIGTHRILSNDVKL
                     PKLGLVVVDEEQRFGVGHKEKLKQLKKKVDVLTLSATPIPRTLNMSLLGMRDMSVIET
                     PPRDRLAINTQVVQFSESVIKSAIELEMARSGQVFFVHNRVETIDAIAAHIKKIVPNA
                     RIIVAHGQMGEKEMEQAVLDFVAFKYDVLVATTIIENGIDIPRANTIIINRADNYGLS
                     QLYQLRGRVGRSNRRAYAYLLIPSELELTPIARRRLSAIREFSDLGAGFRIAALDLEL
                     RGAGNILGGHQSGHLDTLGFDLYVKMLERTIREIRGEEIEDETSVSINLGVDVSIPQD
                     YINETGQRLRTYKRISSAGSESDLRQIYSEINDRYGKMPESVECLFDYAGLRRLAEEL
                     RLVSIDKTRDGFAVKLNENSKIAPEKLMDFLSKNEGSTFSPNGILRVITNETNVVEAA
                     RKVLATISSQS"
BASE COUNT          547 a          665 c          620 g          624 t
ORIGIN      
        1 aaaagagttt attgaataaa attattttag ctctggctgc tgatagttgc cagaactttc
       61 cgcgccgctt cgacgacatt ggtttcattc gtaatcacgc gcaggattcc gttcggcgaa
      121 aaggtcgagc cttcgttttt cgataaaaag tccatcaatt tttccggcgc gatttttgag
      181 ttctcgttta atttaacggc aaagccgtcg cgcgttttat caatcgaaac gagccgcagc
      241 tcttccgcca aacgccgcaa tccggcgtaa tcgaacaggc attcgaccga ttcgggcatt
      301 ttgccgtagc ggtcgttgat ttccgaataa atctggcgca gatcgctttc cgaaccggct
      361 gacgagatgc gtttgtaggt gcgcaggcgc tgcccggtct cgttgatgta atcctgcgga
      421 atcgaaacgt ccacgccgag gtttatcgaa acgctcgttt cgtcctcgat ttcctcgccg
      481 cgaatttcgc gaatagtgcg ttcgagcatt ttcacgtaca aatcgaagcc gagcgtatcg
      541 agatgcccgg attgatgacc gcctaaaata tttcccgcgc cgcgcagctc caaatccagc
      601 gccgcgatgc gaaagcccgc gcccaaatcg gaaaattcgc gaatcgccga cagacgccgg
      661 cgcgcaatcg gcgtcagttc caattccgac ggaatcaaaa gataagcgta agcgcgacga
      721 ttcgaacgcc cgacgcgccc gcgcagctgg taaagctgcg aaagcccgta attgtcggcg
      781 cgattgatga taatcgtgtt ggcgcgcggg atgtcgatgc cgttttcgat aatcgtcgtg
      841 gcgaccagaa cgtcgtattt gaaagcgacg aaatccagaa ccgcctgctc catttccttt
      901 tcgcccatct gcccgtgcgc gacgatgatg cgggcgttcg gcacgatttt tttgatgtgc
      961 gcggcgatgg cgtcaatcgt ctcgacgcgg ttgtgaacaa agaaaacctg cccgctgcga
     1021 gccatttcca gttcaatcgc ggatttgatg acgctttcgg aaaactgcac gacctgcgtg
     1081 ttaatcgcca atctgtcgcg cggcggcgtt tcgatgaccg acatatcgcg cattccgagc
     1141 agcgacatat tcaaagtgcg cggaatcggc gtcgcgctca aagtcaaaac gtcgactttc
     1201 tttttcagct gttttaactt ttctttatgc ccgacgccga aacgctgttc ttcgtcgacg
     1261 acgaccagac cgagcttcgg cagcttcacg tcgtttgaaa ggattcggtg agttccgatt
     1321 aaaatatcga gctcgccttt ttcggctttt tcgacgaccg ctttctgttc tttcggcgag
     1381 cggaaacgcg ataaaagctc catcgagacg ggaaaagcgg caaagcgttt tttgaagctc
     1441 tcgaaatgct ggtaagccag aacggtcgtc ggcgcgagaa tggctacttg tttggaatcc
     1501 atcacggctt taaaagcggc gcgcatcgcc acctcggttt tcccgtagcc gacgtcgccg
     1561 acgattaatc tatccatcgg cgtcaaagtt tccatatcgc ttttgacggc ttcaatcgtc
     1621 gtcgattggt cgggcgtcag ctcaaacggg aaagcgtctt caaattcttc ctgccacggg
     1681 ctgtcggcgg aaaacgcaaa gccctgcacg agctttcttt cggcgtaaag ccgcaaaagc
     1741 tcgtccgcca tatcgcgcat cgcgcgcttg gctttcgcct ttgttttctg ccagccgatt
     1801 ccgcccagac ggtctaagct cggctgcgtc gcctcgcccg aagaataacg cgaaaccaaa
     1861 tccagacgct cgaccggcac gaataatttt gtgttgtcgg cgtaaatcaa aagcataaac
     1921 tcacgctgaa tcccttgcga ttcaatgatt tgcaagcctt cgaagcgccc gatgccgtgg
     1981 tcgacgtgaa cgacgtaatc gcccggcttc aaatcgcgga aatccgagat aaacgcgccg
     2041 agcttggttt tcggtctctg gtctttaatt ttcggtctct gaaattcctg ctgcgtggtc
     2101 tcgccgaaaa tgtcggtttc ggtctgcacg attaaatcga gcgatggaat ttcaaaaccg
     2161 ttcgacaaat cgccgacgag cagcgcgctg ccgggcaaat gagcgtcgta atcgcgcaaa
     2221 atctcggtca atctttccgc taatccctgc gattgcaaaa cgatgatttg attttcgctc
     2281 gcctttgccg gcgattttat ttcattcgca aaatccttta aattcccgtg aaactttctg
     2341 gtcgagcgcg attgaatctc gaattcgacc ggcttttcgg cggttgaaaa caggaaaagc
     2401 ggttttttgt cttcggtttt caatctgaga tttccgattg tctcaaattc gaattt
//
LOCUS       DASWNE010000183         1608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_606247, whole genome shotgun sequence.
ACCESSION   DASWNE010000183 DASWNE010000000
VERSION     DASWNE010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606247"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /locus_tag="VGB68_03835"
     CDS             <1..1188
                     /locus_tag="VGB68_03835"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0048390.1"
                     /translation="IPRGVVELLAEKFNMKQQIIQALREGEGLIVIGYAGNDKSVMDL
                     LRTIPSSHYLFWCVLCGEDENPEDKINDNVKKLLREKDGFLVRTRGFDDVMEQIRQGV
                     GITDDDILSQFRERQEYLTKKLEEAKKSVTVPKASSGKTEASENKTKDEQATEFSAKI
                     GKYSETLSLMFQADEAYKSGNVETAEDFYRRAIELDPNYFSAYNNLAYLLIDNAEKQT
                     EAEKLFRRAIELNPENPGPYINLGLLMERKPERQDEAENFYREAIRVSPNSGNAYTGL
                     ARLYLRRGKLKESSELYEKALLLESEPVYPFLGLANIYKKLNDNAKAKEYIEMARSIT
                     PKDDWFNLASIEAVAGNPDSAIENLKKAIEKNQHVKGFAKISVSLSWINDDPRFKELV
                     GED"
     gene            1366..>1608
                     /locus_tag="VGB68_03840"
     CDS             1366..>1608
                     /locus_tag="VGB68_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0048391.1"
                     /translation="MEFIPLGNLSMLETVNLYKTYTSDGADYEVLKNVNLRIDDGDCV
                     AIIGKSGSGKSTLMHLLACLDAPTSGSILLDGADVSQ"
BASE COUNT          492 a          370 c          375 g          371 t
ORIGIN      
        1 attcccagag gcgtcgtcga gctgctcgcc gaaaaattca atatgaaaca gcaaatcatc
       61 caggctctgc gcgaaggcga aggcttaatc gtcatcggct acgccggaaa cgataaatcc
      121 gtcatggatt tgctgcgtac aatcccgagc agtcactatc ttttctggtg cgttctgtgc
      181 ggcgaagacg aaaaccccga agataagata aacgataacg tcaaaaaact gcttcgcgaa
      241 aaagacggct ttctggttag aacgcgcggc ttcgacgatg ttatggaaca gattcggcaa
      301 ggcgtcggaa tcaccgacga cgacattctc agtcaatttc gcgaacgcca ggaatatttg
      361 acaaaaaagc tcgaagaggc aaaaaagagc gtcaccgtcc cgaaagcttc ttcaggtaaa
      421 accgaagctt ccgaaaataa aacgaaagac gagcaggcaa ccgagttttc agctaaaatc
      481 ggcaaatatt ccgaaacatt atctcttatg tttcaggcgg acgaggctta taagtcgggc
      541 aatgtagaaa cggctgaaga tttttatcgc cgcgcgatcg agcttgaccc gaattatttt
      601 agcgcttaca acaatcttgc ttatctatta atagataacg cggaaaaaca aacggaagcc
      661 gaaaaattgt ttcgccgcgc gattgagctt aacccggaaa accccggtcc ctacattaat
      721 ctcggactcc tgatggaaag aaagccggag cgacaggatg aagctgaaaa tttttaccgg
      781 gaagccattc gggttagtcc gaattccggc aatgcctaca ctggtctcgc cagactttat
      841 ttacgtcgcg gcaagcttaa agaatcttcg gaattatatg aaaaagcttt gctgctggaa
      901 tcagaacccg tatatccgtt cttaggactg gcgaatattt ataaaaaact taacgataat
      961 gccaaagcca aagaatacat cgagatggcg agaagcatta cgcccaaaga tgattggttt
     1021 aatctggcat ccatcgaagc cgtcgccggc aatcccgatt cggcgatcga aaacttaaaa
     1081 aaagcaatag aaaagaatca acacgtcaaa ggctttgcaa aaattagtgt aagtctaagc
     1141 tggataaacg acgacccgcg ttttaaggaa ctggtcggag aagattgatg ctcggataaa
     1201 tcgagcgata aaaatcatca acaagatacc aaaaaagtaa aatccggcgt ggaacagacg
     1261 aatcgctttt tcctttccgc attttacttt ttccgtcccg gctttcggct ttctcttcaa
     1321 aatctttttc acagaacaaa caatccgcgt ttcgcgtagt aaaccgtgga atttattccg
     1381 ctcggaaact tgagtatgct ggaaacggtc aatctttata aaacatatac gagcgacggc
     1441 gcggattacg aggttttgaa aaacgtcaat ctgcgcatcg acgacggcga ctgcgtggcg
     1501 attatcggga aatcgggcag cggcaaatcg actttgatgc atcttctggc gtgtctggac
     1561 gcgccgactt ccggctcgat tctgctggac ggcgcagacg tttcgcag
//
LOCUS       DASWNE010000184         2669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_358030, whole genome shotgun sequence.
ACCESSION   DASWNE010000184 DASWNE010000000
VERSION     DASWNE010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358030"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1533
                     /locus_tag="VGB68_03845"
     CDS             <1..1533
                     /locus_tag="VGB68_03845"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEY0048392.1"
                     /translation="HYEMSFLLPHFLKEGRGKLAVYFTRVFNPVWTYPDGFSWIEALS
                     DEKKIGMHIALTPTWNETAYFADYVLPMGHASERHDLTSYETHSGMWISFRQPVLREA
                     ARKMGKDVEFTYETNPGEVWEEDEFWFELSWRIDPDGALGIRKHFISPYRPGEKITID
                     EYYRYIFENTKGLPEAAAAENLTPLDYMRKYGAFEVERAVYNRHLEPLAESELEGAQI
                     DKDTGSVRKNDKTIGVVIDGIPRVGFNSPSRKQEFYSATTKDWGWSEYAVPTYVKSHI
                     HPENLGENEIVLVPTFRLPTLIHTRSGNAKWLAEISHRNPLWMHTIDGERWGIKTGDL
                     VRISTDIGYFVNRAWVTEGIKPGIVACSHHIGRWRRAQDATNNRWSTNQVSIDEVEKG
                     KWKMTVVEGIGAYKSSDPDSSRIFWTDGGVHQNITFPVHPDPVSGMHCWHQRVRVEKA
                     HDGDKYGDVYVDTEKSFAIYKEWLAMTRPAPGPNGLRRPLWLARPLRPSDEMFYVGNA
                     KT"
     gene            1581..2033
                     /locus_tag="VGB68_03850"
     CDS             1581..2033
                     /locus_tag="VGB68_03850"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY0048393.1"
                     /translation="MRILISTDGSDFSRAAVEKCFQIIVSPENTAIKIVSVFEVIEPM
                     DISISPEFSQELESSARKKAQEFAEQAVARVKERFPNIDLTTQVSKGAPDQMLIEEAK
                     KWSADLIVIGSHGRGFWGRMLLGSITDSLVHHAPCSVLVVRGTNDQNA"
     gene            complement(2075..>2669)
                     /locus_tag="VGB68_03855"
     CDS             complement(2075..>2669)
                     /locus_tag="VGB68_03855"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0048394.1"
                     /translation="IATNLLEQARARAESEGLTIRFDEGDAENLPYADGAFDEIVSMF
                     GAMFAPRPELVAAELVRVCRRGGRIAMANWTPEGFIGQMFKITGKYVPPPDMPSPLKW
                     GDEETVRERLQREGIADLQLTRRRCPFRYPFAPAGVVESFRLYYGPTQRAFAALDAAG
                     QAALRNDLEKLWAEHNQAKDDATYVEGEYLEVIATRN"
BASE COUNT          640 a          710 c          738 g          581 t
ORIGIN      
        1 cattatgaaa tgagttttct gctgccgcat tttttgaaag aaggacgcgg aaaactggcg
       61 gtttatttta cgcgcgtttt taatccggtc tggacctatc cggacggttt tagttggatt
      121 gaagcgctga gcgacgagaa aaaaatcgga atgcatatcg ctttgacgcc gacgtggaac
      181 gaaacggctt atttcgccga ttacgttttg ccgatggggc acgcctcgga aagacacgat
      241 ttgacgagct acgagacgca ctcgggaatg tggatttcat ttcgccagcc ggttctgcgc
      301 gaagcggcgc ggaagatggg caaagacgtg gaatttacat acgagacgaa tccgggcgag
      361 gtttgggaag aagatgaatt ctggtttgaa ttgtcgtggc ggattgaccc ggacggcgcg
      421 cttggaatca gaaaacattt catctcgccg tatcgcccgg gcgaaaaaat cacgattgac
      481 gaatattacc ggtatatttt tgaaaacacc aaaggcttgc ccgaagccgc agccgcggaa
      541 aatttaacgc cgctcgatta tatgcgaaaa tacggcgcgt tcgaggtcga acgagcggtt
      601 tacaacaggc atctggaacc gctcgccgaa agcgagctgg aaggcgcgca aatcgacaag
      661 gacaccggca gcgtgcggaa aaacgacaag acaatcggcg tcgtgattga cggcattccg
      721 cgcgtcggct ttaattcgcc gtccagaaaa caggaatttt attccgccac gaccaaagat
      781 tggggctggt ctgaatacgc cgttccgact tatgttaaaa gccatattca tcccgaaaat
      841 ctcggtgaaa acgagattgt cctggttccg acatttcgcc ttccgacttt gattcacacg
      901 cgctccggca acgccaaatg gctggcggag atttcgcatc gcaacccgct ctggatgcac
      961 acgattgacg gcgagcgctg gggaataaaa accggcgacc tcgtgcgcat cagcaccgac
     1021 atcggctatt tcgtcaatcg cgcgtgggtg acggaaggaa tcaagccggg aatcgtcgcc
     1081 tgctcgcatc acatcgggcg ctggcggcgc gcgcaggacg cgaccaacaa tcgctggtcg
     1141 acgaatcagg tttcgattga cgaagtcgaa aaaggaaaat ggaaaatgac cgtcgtcgag
     1201 ggcatcggcg cgtataaaag ctccgacccg gacagctcgc gaattttctg gacggacggc
     1261 ggcgttcatc aaaacatcac ttttcccgtt catcccgacc cggtttcggg aatgcattgc
     1321 tggcatcagc gtgtcagagt cgaaaaagcg cacgacgggg ataaatacgg cgacgtttac
     1381 gtcgatacgg aaaaatcttt cgccatttac aaggaatggc tggcgatgac gcgtcccgcg
     1441 ccgggaccga acgggctgcg ccgaccgctc tggctggctc gaccgctcag accgtcggat
     1501 gagatgtttt atgtcggaaa tgccaagacg taaggattag gctcggcggt ttatttttta
     1561 tcgctgaagg tgacaagcga atgagaatct taatttcgac cgacggctcg gatttcagcc
     1621 gcgccgccgt cgaaaaatgc tttcagataa ttgtctcgcc ggaaaatacg gcgataaaaa
     1681 tcgtttccgt ttttgaagtt atagagccga tggatatcag catatcgccg gagttttctc
     1741 aggaactcga aagctcggcg cgcaaaaaag cgcaggaatt tgccgaacaa gccgtcgcgc
     1801 gcgttaagga gcgttttccg aatatcgatt taacgacgca ggtttcaaag ggcgcgcccg
     1861 accaaatgct catcgaagaa gcaaaaaaat ggtcggcgga tttgattgtc atcggctcgc
     1921 acgggcgcgg cttttgggga cgaatgctgc tcggctcgat taccgattcg ctggttcacc
     1981 acgcgccgtg ctcggttctc gtcgtcagag gaacaaacga ccaaaacgcc tgaggtcttt
     2041 aaatttcttt ccccgaagga aatgttttcg acctttagtt tctcgtggca ataacttcga
     2101 gatattcacc ttcgacataa gtcgcgtcgt ccttcgcttg attatgctcc gcccacagct
     2161 tctccaggtc gtttctcagc gcagcttgtc cggcagcgtc aagcgcggca aacgcccgtt
     2221 gcgtcggacc gtaatacagg cggaacgatt cgaccacccc ggcgggcgca aacgggtagc
     2281 ggaacggaca cctccgccgc gttaattgta aatccgcaat tccttcgcgc tgtagtcgct
     2341 cgcgcacggt ttcctcgtcg ccccatttga gaggcgaagg catatcgggc ggcggcacgt
     2401 atttgcccgt aattttaaac atctgaccga taaagccttc cggcgtccaa tttgccatcg
     2461 caatccgtcc gccgcgtcgg cagacgcgca ccaactcagc cgcgaccagc tcgggacgcg
     2521 gcgcaaacat cgctccgaac atactcacga tttcgtcaaa ggctccgtcc gcgtatggca
     2581 gattctcggc gtcgccttcg tcgaatcgaa tcgtcaagcc ttcggattcg gcacgcgccc
     2641 gcgcctgttc aagcaaattc gtggcgatg
//
LOCUS       DASWNE010000185         3355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_217191, whole genome shotgun sequence.
ACCESSION   DASWNE010000185 DASWNE010000000
VERSION     DASWNE010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217191"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VGB68_03860"
     CDS             <1..210
                     /locus_tag="VGB68_03860"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0048395.1"
                     /translation="SLRMKVIAEGVETQAQYNQLKDLNCEGAQGYFFGKAMNFEEAAI
                     YFREQNRQTIPLPPLEEISVVSTLQ"
     gene            complement(314..2224)
                     /locus_tag="VGB68_03865"
     CDS             complement(314..2224)
                     /locus_tag="VGB68_03865"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY0048396.1"
                     /translation="MKYEKYVSLIKKLEVYAEKNPKAYQYRVAALAALGYFYILGLIF
                     LCILLPLLLVVAFIFYPDVMLRAFFQLLKLWWILLPALAVFFSFLSGAIRSLMAKVPE
                     PAGNEIERADAPQLFEFVEKTCRELNAQMPEKILVNDDFNASVVTTPRFGIFGRKVFL
                     NLGLPLMYALSPEQFRAVVTHEIGHISGKHGKFSKWTYQLHETWNRFIESQEMQEHKF
                     SVLYEKFVKWFFPYFTAYSFVLMRRHEREADAYTVQLAGAKPLGEALISLETKSAMLS
                     QDFWQKVFEENTKNDNPPTELFSRMALAFRKQDAEKETKNLEKFLDIKTDYSDTHPSL
                     AERLHAIGYWTEENLPKLPEPNRENAAEIFFSPNRLENFVRQYDREWQERVGKNWKTN
                     YEYLQNSQKRLDELSQKETLTPEELFEKALLLVENTTMRVDAIAVFRELLEIQPEHAE
                     ANYILGGILLEQSDESGLEYLNQAMRLDSRFKFAASETAFNYLRLKGRHDEAKHYVEN
                     IENEREIMQKAENERNSVLATDSFEPHKLTDEVLEKVTKKLSYHEEIEAAYLVCKTVQ
                     YYPEIPFHVLFLKMNAHILKSSGVSAQDVVDTIGNQLGEFGILCIYTFGSREAQYESK
                     IQAVEKARIYGG"
     gene            complement(2321..3124)
                     /gene="sdhB"
                     /locus_tag="VGB68_03870"
     CDS             complement(2321..3124)
                     /gene="sdhB"
                     /locus_tag="VGB68_03870"
                     /inference="COORDINATES: protein motif:HMM:NF006391.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEY0048397.1"
                     /translation="MNEANVHEDKKRLENPPKTVEIRIARREKTGAQQYTEIFEIPYR
                     RNLNVISALMEIRKNPVTKDGKQTTPPVWDMNCLEQVCGICTMVINGRVRQSCSALID
                     DLLISSGGNTITLEPMSKFPNVRDLVVDRTQMFEHLKRTKVWIELDGTHDLGPAPRMS
                     QETVEERYAYSRCMTCGCCLEACPQYHEDNYIGPQAIAQVRYQNMHPTGKMNRDERLE
                     ALMQEDGITNCGNAQNCVQVCPMSIPLTRAIYETNREVTIHGLFGWLRK"
     gene            complement(3121..>3355)
                     /locus_tag="VGB68_03875"
     CDS             complement(3121..>3355)
                     /locus_tag="VGB68_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048398.1"
                     /translation="FDRAEKFLSYQTLPSLEEYVLISQEKPLVEQHIKREDGNWIYQA
                     TIGTESSVTFPSVEVTLSLDEIYDLVELEEENL"
BASE COUNT          796 a          849 c          803 g          907 t
ORIGIN      
        1 agcctgcgaa tgaaagtcat cgccgaaggc gtggaaacgc aggctcaata caatcaactg
       61 aaagatttaa actgcgaagg cgcgcagggt tatttcttcg gcaaagcgat gaattttgaa
      121 gaagccgcaa tttatttcag agagcaaaac cggcagacaa tcccgcttcc gccgctggaa
      181 gaaatttcgg tcgtctcgac gctgcaatag tttttggtta attaaaagaa tgaccgccaa
      241 gattttagcg gcgtttaaaa tttaccgggc gcgaataaaa caaaggttat ttccttgagc
      301 cgcttttttt gctttaaccg ccgtatattc gagctttctc gaccgcctgt attttgctct
      361 catactgcgc ctcccggctg ccgaaagtgt agatacataa aatcccgaat tcgcccaact
      421 ggtttccaat cgtatccacg acgtcctgag cgctgacgcc ggagcttttc aaaatatggg
      481 cattcatctt tagaaacaaa acgtgaaacg gaatttccgg gtaatattga accgttttac
      541 aaaccaggta agccgcttcg atttcctcgt gatacgaaag ttttttcgtc accttctcca
      601 agacttcgtc cgttaattta tgaggctcga agctgtcggt cgccaaaacg ctgtttcttt
      661 cgttttcggc tttttgcata atttcccgtt cgttctcgat attttcgacg taatgtttcg
      721 cttcgtcgtg acgacctttc aggcgcaggt agttaaaagc cgtttcgctt gccgcaaatt
      781 tgaaacgcga gtcgaggcgc atcgcttggt tcagatattc aagcccgctt tcgtccgatt
      841 gttcgagcag gattccgccc agaatgtaat tggcttcggc gtgttccggc tggatttcca
      901 gaagctctcg aaaaacagca atcgcgtcaa cccgcatagt ggtgttttcg accagcaaca
      961 gagctttttc gaataattct tcgggcgtta aggtttcttt ctgcgaaagc tcgtcgagtc
     1021 gtttctgcga attctgcaaa tattcgtagt tggttttcca gtttttcccg actctttcct
     1081 gccactcgcg gtcatactgc cggacaaaat tttcgagccg gttcggactg aaaaaaattt
     1141 ccgccgcatt ttccctgttc ggttcgggca gcttcggcaa attttcttcc gtccagtaac
     1201 cgatggcgtg caggcgttcg gctaaagaag gatgcgtgtc gctgtagtcg gttttgatgt
     1261 caagaaactt ttccagattt ttcgtttcct tctcggcgtc ctgtttgcga aaagcgagcg
     1321 ccatccggct gaacaattcg gtcggcggat tgtcgttttt tgtgttttcc tcaaaaactt
     1381 tctgccagaa gtcctgcgag agcatcgccg attttgtttc cagacttatc agcgcttcgc
     1441 cgagcggctt cgctccggcg agctgcaccg tgtaggcgtc cgcctcgcgc tcgtgacgcc
     1501 gcatcagcac aaaagagtaa gccgtgaaat aaggaaaaaa ccatttgacg aatttttcgt
     1561 agagcaccga aaatttgtgt tcctgcatct cctgcgactc gataaatctg ttccaggtct
     1621 cgtgcagctg ataagtccat ttggaaaatt tgccgtgttt gcccgaaata tgcccgattt
     1681 cgtgagtcac gacggcgcga aactgctccg gcgaaagcgc gtacattaaa ggcagaccga
     1741 gattcaaaaa gaccttccgc ccgaaaatac cgaaacgcgg tgtggtaacg accgaagcgt
     1801 taaaatcgtc gttgaccaga attttttccg gcatttgcgc gttcagctcg cggcaggtct
     1861 tttcgacaaa ttcgaacagt tgcggcgcgt cagcccgctc gatttcgttg cccgccggct
     1921 cgggaacttt cgccatcagc gagcgaatcg cgcccgacag aaagctgaaa aagacagcca
     1981 gcgcgggcag caaaatccac cacagcttga gaagctgaaa aaaagcgcga agcatcacgt
     2041 ccgggtagaa gataaaggca acgaccagga gaagcggcag cagaatacac aaaaaaatca
     2101 gcccgagaat gtagaaatag ccgagcgccg ccagagcggc gacgcggtat tgatacgctt
     2161 tcggattctt ttcggcgtaa acttcgagtt tttttataag tgagacgtat ttttcgtatt
     2221 tcatacggtt caggaaatca aatcacaaat catagcaaaa ataaaaaaaa ggtaaaagcg
     2281 gaccgggctt ttaccttttt cgttttattt atacacgatt ttattttctg agccaaccga
     2341 aaagcccgtg aatcgtcact tccctgttcg tttcgtaaat cgcgcgggtc agcggaatcg
     2401 acatcgggca aacctgaacg caattttgcg cgttgccgca gttcgtaatt ccgtcttcct
     2461 gcattaaagc ttcgagccgc tcgtcgcggt tcatttttcc ggtcgggtgc atattctgat
     2521 agcgaacctg cgcgatcgcc tgcggaccga tgtaattatc ttcgtgatac tgcggacagg
     2581 cttccagaca acagccgcac gtcatacagc gcgagtaagc gtagcgttcc tcgaccgttt
     2641 cctgcgacat tcgcggcgcg ggtccgaggt cgtgcgtgcc gtccagctca atccagacct
     2701 tggttcgctt gagatgctcg aacatctgcg tgcggtcaac gaccaaatcg cgcacgttcg
     2761 gaaactttga catcggctcc agggtaatcg tgtttccgcc cgaagagatt aataaatcgt
     2821 caatcaaagc cgagcaggat tggcgcacgc gaccgttgat gaccatcgtg cagatgccgc
     2881 agacctgttc cagacagttc atatcccaga cgggcggcgt ggtttgttta ccgtctttcg
     2941 tcaccggatt tttacggatt tccattaacg cggaaatcac gttcagattt cgccggtacg
     3001 ggatttcaaa aatttccgta tattgctgcg cgccggtttt ctcgcgccgc gcgatgcgaa
     3061 tctcgactgt tttcggcgga ttttccagcc gctttttgtc ctcgtgcacg tttgcttcgt
     3121 tcataaattt tcttcttcaa gttcgactaa atcgtaaatc tcatctaaac tcagcgtcac
     3181 ttcgactgat ggaaaagtca ccgagctttc tgttccgatg gttgcttgat atatccaatt
     3241 tccgtcttcg cgtttaatgt gttgctcgac caaaggcttt tcctgcgaaa tcaaaacgta
     3301 ttcttccagc gacggcaggg tttgataaga caaaaatttt tcggcgcggt caaag
//
LOCUS       DASWNE010000186         1799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_111596, whole genome shotgun sequence.
ACCESSION   DASWNE010000186 DASWNE010000000
VERSION     DASWNE010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111596"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..>1799
                     /locus_tag="VGB68_03880"
     CDS             195..>1799
                     /locus_tag="VGB68_03880"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0048399.1"
                     /translation="MNVNLKSLFGRLNDASRGALEGAAALCLSRTNYDVEIEHILAKL
                     LEANDTDFEKILRHYECNPDRVTKDVSAALDRLKTGNSRTPGLSDRLPSWFQDAWLLA
                     SVEYGAARVRSGHLILALLANDSLARISREISREFNHISVESLKTKLNDITAGSAEDR
                     EATSLSETGAASSDGAPVAGGAGGKTKALDQYTEDLTAKARAGKIDPILGRDFEVRQL
                     VDILTRRRQNNPILTGEAGVGKTAVVEGFANRIAAGDVPEPLKNVIVRTLDLGLLQAG
                     ASVKGEFENRLKSVIDEVKSSPQPIILFIDEAHTMIGAGGQAGQNDAANLLKPALARG
                     ELRTIAATTWAEYKKYFEKDAALARRFQVVKVEEPDEEKAIGMMRGLVDKMEEHHNVR
                     ILDEAIVDCVKLSHRYITGRQLPDKSVSVLDTACAKVAIGQGATPGAIEDSTKFINRL
                     DTEIASLERELVTGAQHDERLGELKELKAREEEKLAVYQEQWTKEKELIEKIRNIRLK
                     LEMSRLDAKPVTSQNGNGNGNEQSAAA"
BASE COUNT          460 a          477 c          513 g          349 t
ORIGIN      
        1 gtcgaacaac ttctgaaaat tcacgaagtt cagcttttgt cttatcttcg gttatataaa
       61 aagtctctcg gcttaattat caactttcac gttccggtat tgaaaagcgg gcttagacga
      121 atagtaaata attttcaaga aaactccgcg tctccgcgtc tctgcgttga aaatctttta
      181 aggagcaatg aatcatgaac gtaaatttaa aatctttatt tggcagatta aacgacgctt
      241 ctcgcggcgc gctcgaaggc gcggcggcgc tttgcctttc gcggacgaat tacgatgtgg
      301 aaatcgaaca cattctggcg aagcttctgg aagcaaacga cacggatttc gagaaaatcc
      361 tgcgtcatta cgaatgcaat cccgaccgcg tgacgaaaga cgtctccgcc gcgctcgacc
      421 gtctgaaaac gggaaattcg cgcacgcccg gcttgagcga ccgcctgccg agctggtttc
      481 aggacgcctg gctgctggct tcggtcgaat acggcgcggc gcgcgtccgc tccggtcatc
      541 tgattctggc gctgcttgcc aacgacagcc tggcgcgcat ctcccgcgag atttcgcgcg
      601 aatttaatca catctcggtc gaatcgctga agacgaaact aaacgacatc acggcgggct
      661 cggcggaaga ccgcgaagcg accagccttt ccgaaacggg cgcggcttct tctgacggcg
      721 cgccggtcgc cggcggcgct ggcggcaaaa cgaaagccct cgaccaatac accgaagatt
      781 tgacggcgaa agcgcgcgcc ggaaaaatcg acccgattct cggacgcgat tttgaagtgc
      841 ggcagcttgt cgacatcctg acgcgccgac gccagaacaa tccgattctg acgggcgaag
      901 cgggcgtcgg caaaacggcg gtcgtcgaag gcttcgccaa tcgcatcgcg gcgggcgacg
      961 ttcccgaacc gcttaaaaac gtgattgttc gaacgctgga tttaggactt ctgcaagcgg
     1021 gcgcgagcgt caaaggcgaa tttgaaaatc gtctgaaatc ggtaattgac gaggtaaaat
     1081 cttcgccgca accgattatt ttatttatcg acgaagcgca cacgatgatc ggcgcgggcg
     1141 ggcaagccgg gcaaaacgac gccgcaaatc ttttaaaacc cgcacttgcg cgcggcgagc
     1201 tgcgcacgat tgccgcgacg acgtgggcgg aatacaaaaa atattttgaa aaggacgcgg
     1261 ctctggcgcg gcgcttccag gtcgtcaagg tcgaagaacc ggacgaagaa aaagccatcg
     1321 gaatgatgcg cgggctggtc gataaaatgg aagagcatca caacgtccga atcttagacg
     1381 aagcgattgt cgattgcgtc aagctttcgc atcgttatat tacgggtcgc caattgcctg
     1441 acaaatcggt ttcggttctc gacacggctt gcgcgaaagt cgctatcgga cagggcgcga
     1501 cgccgggcgc gattgaagat tcgaccaagt ttatcaatcg cctggacacg gaaatcgcct
     1561 cgctcgagcg cgagctggtg acgggcgcgc agcacgacga gcggctcggc gagctgaaag
     1621 aattaaaagc gcgcgaggaa gaaaagctcg ccgtatatca ggagcaatgg acgaaagaaa
     1681 aggagctgat tgaaaaaatc cgcaacattc gcctgaagct cgaaatgtcg cggctcgatg
     1741 cgaaacccgt gacgagccaa aacggaaacg ggaatggtaa tgagcaatcc gccgccgcg
//
LOCUS       DASWNE010000187         4294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_288380, whole genome shotgun sequence.
ACCESSION   DASWNE010000187 DASWNE010000000
VERSION     DASWNE010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288380"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..513
                     /locus_tag="VGB68_03885"
     CDS             37..513
                     /locus_tag="VGB68_03885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2169 family four-helix-bundle protein"
                     /protein_id="HEY0048400.1"
                     /translation="MANDSVVTTLNNLIETCKDGQEGFQQAAEGVQDSSLKSLFYEYS
                     QQRAKFVGELQGLVRDLGGEAETSSSIAGALHRGWINIKSAVTGRDDAAILNECERGE
                     DIAKSAYKDALAADLPSNVTSVIQEQATQVQQAHDRIRGLRDAANNEASSTATTGH"
     gene            complement(710..1753)
                     /locus_tag="VGB68_03890"
     CDS             complement(710..1753)
                     /locus_tag="VGB68_03890"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEY0048401.1"
                     /translation="MNENAENLISIQNLKVHYKTGGGLFKETKHVKAVDGVSLDIKKG
                     ETLGLVGESGCGKSTLGKALLRLTEPTGGKVIYNGKDLAHLKQNQMRDQRKHLQMIFQ
                     DPYASLNPRMTVGQIIGEPIRTFGLSKGGSVEAKVQDLMETVGLSRRFIKRYPHEFSG
                     GQRQRIGIARALAVDPEFIVADEPISALDVSIQAQIMNLMARLQKEKDLTYLFISHDL
                     RAIRHVADRVAVMYLGKIVELGDAKEVYREPLMPYTIALISAVPVPDPKIEAKRERII
                     LTGDVPSPINPPRGCHFHTRCPYAIEECKKIEPLLAEIKPKHFAACIRISPEKPHIEK
                     NAGAGVIGAGKPD"
     gene            complement(1765..2823)
                     /locus_tag="VGB68_03895"
     CDS             complement(1765..2823)
                     /locus_tag="VGB68_03895"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0048402.1"
                     /translation="MNENNGSILKVNNLKTYFQTEDGVVKAVDGITFELKKGETLGIV
                     GESGSGKSVTNLSVMRLIPEPPGKIVDGTIIFDGIDVRKLSLDDVRNIRGKRIAMIFQ
                     DPMTSLNPYLRISTQLMEVTRLHLGHTKEQAYQHAIKMLKTVGIPDAENRIDGYPHEF
                     SGGMRQRVMIAMALSCDPELLIADEPTTALDVTIQAQILELIKDLKARMGTSVILITH
                     DLGVVAGMTEKIIVMYAGKVFEQAPTIELFATPANPYTKGLLKSVPDPAHEDGTELYQ
                     IPGLPPDVAHLPPGCPFSPRCDYAQEICHREYPPFVQINENHHSLCHFAREVYAESVR
                     ENENKASAEIDLQMKVNE"
     gene            complement(2831..3295)
                     /locus_tag="VGB68_03900"
     CDS             complement(2831..3295)
                     /locus_tag="VGB68_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048403.1"
                     /translation="MTAAAVNAQNKKLSTPKLNVAKEGVGIEGIVVGKSTMDDVIKRF
                     GRDYNWTTNKKYSYQMTYPKLGLSFYICQADKRKEIFVIEIKSPYRAKTSRGVVLGQS
                     TVEDIEKTYGKLKSGLEYRGISFYYNRVGRKRIVSSIDVTENSGIRQCKENK"
     gene            complement(3367..>4294)
                     /locus_tag="VGB68_03905"
     CDS             complement(3367..>4294)
                     /locus_tag="VGB68_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0048404.1"
                     /translation="ASLWRDAWKRLLKNKLAVFGLVVIVLIAIAVTVGPFFLQKLAGI
                     TPSGMPASGDLFKSSPPSWRNWMGTDDAGRDIFARVLQGGQISLMVGIISTIVSLLVG
                     VSYGAIAGYMGGRIDNLMMRFVDILYSIPYILIVIVLLSVFGGPNTPQWIQSMANNFG
                     GGQGLSQIFLLFFALGLVSWLTMARVVRGQILSLKNQEFVMAARATGVSTPAIIFRHL
                     VPNALGPVIVYATLTIPSVMLTEAFLSFLGIGVQAPYASWGSLANEGIKNISVFPWQL
                     IFPGVTMALTLFSLNFLGDGLRDALDPQTRKF"
BASE COUNT         1025 a         1136 c         1095 g         1038 t
ORIGIN      
        1 gactaatttt ctctaatcgc aaggaggtta attttaatgg caaacgatag cgtggttaca
       61 acgctcaata atctgattga aacctgtaag gacggtcagg aaggttttca gcaggcggct
      121 gaaggcgttc aggattcgag cctgaaatct ttattttacg aatacagcca gcagcgcgcg
      181 aaatttgtgg gtgaattgca gggcttggtt cgcgatttag gcggcgaggc ggaaacgtct
      241 tcgagcattg ccggcgcgct tcatcgcggc tggattaata ttaaatccgc cgtcaccgga
      301 cgcgacgacg cggcgattct aaacgaatgc gagcgcggcg aagacatcgc gaaaagcgct
      361 tataaagatg ctctggcggc agatttaccg tcaaacgtca cttcggtcat tcaagaacag
      421 gcaacacaag ttcagcaggc gcacgataga attcgcgggc tgcgcgatgc ggcaaacaac
      481 gaagcatcgt cgaccgcgac aaccggtcat taaccgtttt aaactttggc gaacattact
      541 cctttggaaa ttaaagaaag aggctgaccg cgcggttagc ctctttcttt attcgggata
      601 gaaaattcaa cacgaaagca cgaagcacgc aaagaaagca caaagcacac aaagcactta
      661 tccacggtca gtttctttct ctcaaaattt ctatgattct atttcttaat taatccggct
      721 ttcccgcgcc gatgacgccc gcgccggcgt ttttctcgat atgaggtttt tccggcgaaa
      781 tgcgtatgca agccgcgaaa tgtttcggtt taatctccgc cagcagcggc tcgatttttt
      841 tacattcttc aatcgcatac gggcagcgcg tgtgaaaatg acagccgcgc ggcggattta
      901 tcggcgaagg aacatcgccc gtcagaataa tgcgctcgcg tttggcttcg attttcgggt
      961 cgggcacggg aacggcggaa atcagcgcta tcgtgtaggg catcagaggc tcgcgataaa
     1021 cttctttggc gtcgccgagc tcgacgattt ttccgagata catcacggcg actctgtcgg
     1081 caacgtggcg aatcgcccgc aaatcgtgcg agatgaaaag ataagttaaa tctttttcct
     1141 tttgcaggcg cgccatcaga ttcataatct gcgcctgaat cgaaacgtcg agcgcggaaa
     1201 tcggctcgtc cgccacgata aattccggat cgaccgccag cgcgcgggcg atgccgattc
     1261 tctggcgctg accgccggaa aattcgtgcg ggtagcgttt gataaaacga cggctcaagc
     1321 cgaccgtttc catcaaatcc tgcactttcg cctcgaccga accgcctttg ctcaagccga
     1381 atgtgcgaat cggctcgccg ataatctgcc cgaccgtcat acgcggattc aagctggcat
     1441 acgggtcttg aaaaatcatc tgcagatgct tgcgctggtc gcgcatctgg ttttgtttta
     1501 aatgcgccaa atccttgccg ttgtaaatca cttttccgcc ggtcggctcg gtcagacgca
     1561 aaagcgcttt gccgagggtc gatttgccgc agcccgattc gccgaccagc ccgagcgttt
     1621 cgcctttttt gatgtccaga gacacgccgt cgacggcttt gacgtgcttt gtttccttga
     1681 aaagcccgcc gcccgttttg taatggactt tcaggttttg aatcgaaatt aaattttcgg
     1741 cgttttcatt cataaataat gttgttattc attaaccttc atctgcaaat cgatttcggc
     1801 agaagcttta ttttcatttt ccctgaccga ttcggcgtaa acttcgcgag cgaaatgaca
     1861 cagcgaatgg tgattttcgt ttatctgcac aaacggcgga tattcgcgat gacagatttc
     1921 ctgcgcgtaa tcgcagcgcg gcgaaaacgg gcagcccggc ggcaggtgcg cgacatcggg
     1981 cggaagaccg ggaatctggt agagttccgt cccgtcttcg tgcgccgggt cgggaacgga
     2041 tttcagcaag cctttcgtat aaggattggc gggcgtggcg aaaagctcga tggtcggcgc
     2101 ctgctcgaaa accttgcccg catacatcac gataattttt tcggtcattc cggcgacgac
     2161 gcccaaatcg tgcgtaatca gaataacgct cgtccccatt ctcgctttca ggtctttgat
     2221 aagctccaga atctgcgcct gaatcgtcac gtcgagcgcg gtcgtcggct cgtcggcaat
     2281 caacagttcc gggtcgcacg acaaagccat cgcaatcatc acgcgctggc gcattccgcc
     2341 ggaaaattcg tgcgggtagc cgtcgatgcg attttccgcg tcgggaatgc cgacggtttt
     2401 cagcattttt atggcgtgtt gatacgcctg ctctttcgtg tgtccgagat gcaggcgcgt
     2461 gacttccatc aattgcgtgg aaatccgcaa atacgggttg agcgaggtca tcgggtcctg
     2521 gaaaatcatc gcaatgcgct taccgcgaat gttgcgcacg tcgtcgagcg aaagcttgcg
     2581 cacgtcgatg ccgtcgaaaa taatcgtgcc gtccacgatt tttcccggcg gttccggaat
     2641 cagacgcatc accgaaagat tcgtgacgga ctttccggag ccggattcgc cgacgatgcc
     2701 gagcgtttcg ccctttttca gctcgaacgt aattccgtca actgctttga cgacgccgtc
     2761 ttcggtctga aaataagttt tcagattgtt gacttttaaa atgcttccgt tgttttcgtt
     2821 catcgtatgg ttatttgttt tccttgcatt gccggatgcc cgaattttcc gtgacgtcaa
     2881 tcgacgatac gattcttttc ctgccgacgc gattgtaata aaaactgatg cctcgatatt
     2941 cgagcccgct tttcagtttt ccataagttt tttcgatgtc ctcgaccgtg ctttgtccca
     3001 aaacgacgcc gcggctggtt ttggcgcgat aaggcgattt gatttcgatg acgaagattt
     3061 ccttacgctt gtcagcctga cagatgtaaa acgacaaacc cagtttcggg taagtcatct
     3121 ggtaggaata ttttttattg gtcgtccagt tataatccct gccgaatctt ttgataacat
     3181 cgtccatcgt cgatttgccg acgacaatgc cttcgatgcc gactccttct ttggcgacgt
     3241 tcagttttgg cgtcgaaagc tttttgttct gcgcatttac cgctgcggct gtcaaagatg
     3301 aaaaacatat caaagccgaa aatatcgaaa gaaaaaattt tatcctcatc aattagttga
     3361 acgctcctag aattttctcg tctgcgggtc gagcgcgtcg cgtaagccgt cgcccagaaa
     3421 gttcagcgag aaaagtgtca gagccatcgt cacgcccgga aaaataagct gccacggaaa
     3481 aaccgagata ttttttattc cttcgttggc gaggctgccc cacgaggcgt aaggcgcctg
     3541 cacgccgatg cccagaaacg aaagaaacgc ttcggtcagc atcacggaag gaatcgtcag
     3601 cgtcgcgtag acaatcaccg gaccgagcgc gttcggaacc agatgacgaa agataatggc
     3661 gggcgtcgaa acgccggtcg ctcgcgccgc cataacgaat tcctgatttt tgaggcttaa
     3721 aatctgcccg cgcacgacgc gcgccatcgt cagccacgaa accagtccga gcgcgaaaaa
     3781 caaaagaaaa atctggctca agccctgtcc gccgccgaaa ttatttgcca tcgattgaat
     3841 ccattgcggc gtattcggtc cgccgaaaac cgacagcaaa acgatgacga tgagaatata
     3901 gggaatcgaa tacagaatat cgacgaatct catcatcaga ttgtctatgc gcccgcccat
     3961 ataaccggca atcgcgccgt aactgacgcc gaccagaagc gaaacgatcg tcgagataat
     4021 gccgaccatc agcgaaatct gcccgccctg caaaacgcgc gcgaaaatat cgcgtccggc
     4081 gtcgtccgtc cccatccagt ttctccacga aggcggcgac gatttaaaaa ggtcgccgct
     4141 tgccggcatt ccggacggcg taattccggc gagcttttgt agaaagaaag gtccgaccgt
     4201 gacggcgatc gcaatcagga cgataaccac caagccgaaa acggcgagct tgtttttcag
     4261 cagacgcttc cacgcatcgc gccacaagga cgcg
//
LOCUS       DASWNE010000188         2856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_288408, whole genome shotgun sequence.
ACCESSION   DASWNE010000188 DASWNE010000000
VERSION     DASWNE010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288408"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1863
                     /locus_tag="VGB68_03910"
     CDS             <1..1863
                     /locus_tag="VGB68_03910"
                     /inference="COORDINATES: protein motif:HMM:NF017000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase LanC family protein"
                     /protein_id="HEY0048405.1"
                     /translation="LLEKEHRILLKLMHTNLVPKVIDFFQDWEHSFLVMERVEGGQLS
                     TFATRHNVTLKTAPTIEDTRTVYNYFKTIFLQLAKILKTMHENGIVFTDFSPNNVLVN
                     FDTLEVKIIDFEGAYEKGVDAPVPLFTPGFTAPHQLAGHHSSFESDYFALGATMHYFL
                     SPVNQIFAIHPKARYSFIKSVINDVGFPESLYTLISELVDKEPEKRPKPEKVIEFLEK
                     EEEIKAPNFRFDAAEVEATYHKLVKTVPRYILAKADYSRPDRLFPADYAIFATNPLNI
                     AYGACGVAYAINKMEGSVPETVTDWILARNKNPELYPPGLYVGLAGIAWSMLELGLIE
                     PAEEIIKSTFNHRLLYDSMDLYYGIAGWGMANLKFFAELQNEIYLQKAIEAGDFLIKN
                     LSKDEKGCYFETESGIPLGLHHGASGVSLFLLYLYLASKKEIFLEVGRQALDFDINNA
                     SPNIEGGISWKRYVDQGRIVYPYLGQGSAGVGVAVARYYRLLSGDDEKYGEMLEKIYH
                     DTNRKYAVFPGVFSGLSGIGEFLLDLHGVTGRQSHLEAAYRIASGVSLFIIEKEEGVA
                     FPGDELKRISCDFGTGSAGIGLFLHRLVHRGESHFKLDELFAEEKTSLAAGSII"
     gene            1879..>2856
                     /locus_tag="VGB68_03915"
     CDS             1879..>2856
                     /locus_tag="VGB68_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0048406.1"
                     /translation="MFDKVKDSTQTETTSWRGLLSYVIEGNKGKFAFAVLIGLLANGF
                     ISIANPLALKYLFDEGVIRGDFGLFVILGISFVVIFSFWRIWMYFYRIYVQKLKVSVN
                     KRICLKMLHKYYELPYNEVLKKEQGYFLSRIYDEVISTSQPTIDSFLSLSNTLVALVG
                     GSIIVLSMSWRASLTITFAIPLIYLVSRKFGGKIKEQSKIEKEEEAKLRGLLGRTINS
                     YKPLRVFNLKTKVFEKIEDVFDKYSKAYTFRFRTGARYETISGTLMSYAETLAIIGAG
                     YEMLVGRMSFGGYMAFMTAYWTVIGSVKSLFNLVPEMSRLSGSVERLKEF"
BASE COUNT          752 a          655 c          719 g          730 t
ORIGIN      
        1 ctgctggaaa aggaacatcg catcttatta aaactaatgc acaccaatct ggttccgaaa
       61 gtcatagact tttttcagga ttgggaacac tcgtttctgg ttatggaacg agtcgagggc
      121 ggacagcttt cgaccttcgc caccagacac aatgttacgc ttaaaaccgc gccgacgatt
      181 gaagataccc gcacggttta caattatttc aaaaccattt tcctgcagct ggcgaagatt
      241 ctgaaaacga tgcacgaaaa cgggattgtc ttcaccgatt tctcgcctaa taacgtcctc
      301 gtaaatttcg acacgctgga ggtcaagata atcgactttg aaggcgcgta cgagaaaggc
      361 gtcgacgctc cggttccatt attcacgccc ggatttaccg ctccgcacca gctggcgggg
      421 catcattcct cgtttgaaag cgattatttt gctctgggcg cgacgatgca ctattttctg
      481 tcgccggtaa atcaaatctt tgccattcat ccgaaagcca gatacagctt tattaaatcc
      541 gtcataaatg acgtcggttt tcccgaaagt ctttacacgt taataagcga gctggtagac
      601 aaagagccgg aaaaaagacc gaagccggag aaagtaatcg aatttttaga aaaagaagaa
      661 gaaatcaaag cgccgaattt tcgtttcgat gcggctgagg tcgaagcgac ttatcataaa
      721 ctggttaaaa ccgttccccg atacattctg gcgaaagccg actatagtcg acccgaccgg
      781 cttttccccg ccgactacgc tatttttgcc acaaatcctt taaacatcgc gtacggcgcg
      841 tgcggcgtcg cctacgccat caacaaaatg gaaggcagcg tgcccgaaac ggtgaccgac
      901 tggatactgg ctcgaaataa aaatccggaa ttatatccgc cgggcttata tgtcggtctg
      961 gcgggaatcg cctggtctat gctggaactg ggtttaatcg aaccggcgga agagattata
     1021 aaatcaacct tcaaccaccg cctgctttac gactcgatgg atctttacta cggcatcgcc
     1081 ggatggggaa tggcaaatct gaaattcttc gccgagcttc aaaacgagat ttatttacag
     1141 aaggcaatcg aagccggaga ttttttgata aagaatttgt cgaaagatga aaagggctgt
     1201 tatttcgaga ccgagagcgg cattccgctc ggacttcatc acggggcgag cggcgtcagc
     1261 ctgtttttgc tttatttgta tctggcgagt aaaaaggaaa tttttctgga ggtcgggcgg
     1321 caggctctgg attttgatat taacaacgcg agcccgaata tcgaaggcgg aatatcctgg
     1381 aaaagatacg tcgaccaggg aagaatcgtg tacccgtatc tggggcaggg aagcgccggc
     1441 gtcggcgtgg cggttgccag atattaccgt ctgctgtcgg gcgacgacga aaaatacggc
     1501 gagatgctcg aaaaaatcta tcacgataca aacagaaaat acgcggtctt tcccggcgtc
     1561 tttagcggct taagcggtat cggcgagttt cttctggatt tgcacggcgt caccggtcgg
     1621 cagagtcatc tggaggcggc ttaccggatt gcctccggcg tctcgctttt tatcatcgag
     1681 aaagaggaag gcgtcgcttt tccgggcgat gaattaaaga gaatatcctg tgatttcgga
     1741 acgggcagcg ccggaatcgg tcttttcctg catagattag ttcaccgcgg cgaaagccat
     1801 ttcaagcttg acgagctgtt cgcagaggaa aaaaccagtc tggctgccgg cagcattatt
     1861 taaaaactta ggcgagttat gttcgataaa gtgaaagatt cgacacagac tgaaacgacg
     1921 agctggcgag gcttgctctc ttatgttatc gaaggaaaca aaggaaaatt cgcctttgcc
     1981 gtcctgatcg ggctactggc taacggcttt atcagcatag ccaatccgct ggctctcaaa
     2041 tatctgtttg acgaaggcgt tattcgcggc gatttcggtc tgttcgtaat tctgggaatc
     2101 tcttttgtcg tcatcttttc tttctggcga atctggatgt atttttatcg catatacgtg
     2161 cagaaattaa aggtcagcgt taataaaagg atttgcctga aaatgctcca taagtattat
     2221 gaattgcctt acaacgaagt tctcaaaaaa gagcagggat attttttatc cagaatttac
     2281 gacgaagtaa tttccacttc gcagccgacg atagactcgt ttttatcgct ctcgaacacc
     2341 ctggtggcgc tggtcggcgg ttcgattatc gtgctttcga tgtcgtggcg cgcgtccctg
     2401 accatcacct tcgccatccc gctgatttat ttagtctcgc gcaaattcgg cggtaaaata
     2461 aaggaacagt caaaaatcga gaaagaagaa gaagccaaat tgcgcgggct tctggggcgg
     2521 acgattaatt cttataagcc gctgcgggtt tttaatttga aaacgaaagt ctttgagaaa
     2581 atcgaagacg ttttcgataa gtattccaaa gcctacactt tccggtttcg aaccggcgct
     2641 cgatacgaaa ccatcagcgg tacgctgatg tcgtacgccg agacgctggc gatcatcggc
     2701 gccggttatg aaatgctcgt cggcagaatg tcgttcggcg gttatatggc ttttatgacc
     2761 gcttactgga cggttatcgg cagcgtcaaa agcctgttca atttagtgcc cgaaatgtcc
     2821 agattgagcg gttcggtcga gagattaaag gaattt
//
LOCUS       DASWNE010000189         5075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_253426, whole genome shotgun sequence.
ACCESSION   DASWNE010000189 DASWNE010000000
VERSION     DASWNE010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253426"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VGB68_03920"
     CDS             complement(<1..475)
                     /locus_tag="VGB68_03920"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048407.1"
                     /translation="MTATTIEQTIETYGIENWGAGYFSVNRKGNLVVHPAENDTKSAA
                     DVKEIIDNLKKRGINAPVLLRFPQLIVGQIRKLQKAFKNSIKEYEYNSRHMCVYPMKV
                     NQNRAVVEEYLKEGSRYDFGLEAGSKAELYAALAMEQSDDSLLVLNGFKDEEFIRL"
     gene            829..1251
                     /locus_tag="VGB68_03925"
     CDS             829..1251
                     /locus_tag="VGB68_03925"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY0048408.1"
                     /translation="MNNKKQKGFSLIELLIVMVILGILMILTFPYVRKAKDAAEDGNA
                     FASMKTMLAAQYNFYSQNARYAALTELNDSQYGSLGINAGTSLRRGQYTFVMSPVTPT
                     PTELREGFTIIATRPGYGTEPPVTLTLTERGHADFIFD"
     gene            1333..3042
                     /locus_tag="VGB68_03930"
     CDS             1333..3042
                     /locus_tag="VGB68_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048409.1"
                     /translation="MNVFEDLVEELKEENLLEETVIGRNAAQTHAVEYVADASQFQAQ
                     TGETPAEFESTPSFSADFAFEPDEPEAEELFPEEFLSEEKSSDFLFAGHERSENKISE
                     SSFETAAPEPENFAAPKAQVDEAEFYRRRAIDEVTSLQMVEHVFSGVEREQMRILPKP
                     FNDITVKKALHDFLQVSKNSPGNENAQAEFKLMQETESWCSALSHRDKHLSVSALRRY
                     CETTKPSLSDQAIIALARFYRNLPHTESVRSKFDMILTRLFTREVEGDRRELQFDRDD
                     MVRQISELYADWASVPLYSVEDETELVLITVKFDDFLAEAQDAENFDELVKKDFYNRL
                     RLFKESISDKFYAPLVLASAVESNVLIGNRYIELLEKERRHTSAEAIESQYSFLLDQA
                     ISDATSKTLQLVELLKEKTEVVVVEPKTLDEPKETLAVPEIKVERKKTRQSRVGFFGI
                     SQWLATALVSVILLSAALYFYVEYFSTRRAAPSVQKVNIENSAFKDYLQTARINQETF
                     IGVVNPAWEVINEDKKQEILDKLMNVGREKGFTKVHLLNNQGETVGYATEAEAKVINP
                     GSI"
     gene            3233..4654
                     /gene="glnA"
                     /locus_tag="VGB68_03935"
     CDS             3233..4654
                     /gene="glnA"
                     /locus_tag="VGB68_03935"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEY0048410.1"
                     /translation="MNAKNVLNYAADQGAKFVSVRFTDLPGAWHHLTYPIGQLTEDSF
                     EDGFGFDASSLRGWAAINESDMLLVPDPSRCWVDPFAEETTVCLIANVVDPITKEGYG
                     LDPRSVALRAENYLKFTGIADTVYFGPEAEFFVFDEVFYHNNQNSAGFSVNSEEGHWN
                     SGRSGNDMMPNMGYHIRPKEGYVPVAPLDTLIDLRNAIALVLENVGIHVECHHHEVAT
                     AGQCEIDFKYSNLLHTADNLMMFKYVVKNTAYQYGKTATFMPKPLYGDNGSGMHCHQS
                     LWKDGNPLFSGDQYAGLSEMAKFYIGGLLKHAPAIVAFAAPTTNSYKRLVPGFEAPVN
                     LAYSARNRSAAVRIPMFSQSPKAKRLEFRPPDPACNPYITFAALLMAGLDGVQNRIDP
                     GEPLDKDIYDLSPEELKDVPSLPGSLDEALKALEDDHEFLLKGDVFSTSMIERWITYK
                     REKEIQPLRLRPHPLEFSMYYDI"
     gene            4804..>5075
                     /locus_tag="VGB68_03940"
     CDS             4804..>5075
                     /locus_tag="VGB68_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048411.1"
                     /translation="MSNEAKNEGDGNFFIQGISGSEITINPKHSHSDKKPAPLPKHIT
                     IQSLTPPNYFTGREKVLSDIAETLNARGKAALCGISGLGKSSVVLK"
BASE COUNT         1345 a         1289 c         1227 g         1214 t
ORIGIN      
        1 cgagccggat aaattcctcg tccttaaaac cgtttagaac cagcaggctg tcgtcggatt
       61 gctccatcgc cagcgccgcg taaagctcgg ctttcgagcc ggcttccaag ccgaaatcat
      121 agcgcgagcc ttccttcaga tattcctcga cgacggcgcg gttctggttg accttcatcg
      181 ggtagacgca catatgacgg ctgttatatt cgtattcttt gatggaattt ttaaaggctt
      241 tctgaagctt gcggatttgc ccgacgatca gctgcgggaa acgcagcaga accggcgcgt
      301 tgatgccgcg ttttttcaaa ttgtcgatta tttccttcac gtcggcggct gatttggtat
      361 cattttccgc cggatggacg accagatttc cttttcgatt tacgctgaag taacctgccc
      421 cccaattctc gatgccgtag gtctcgattg tctgttcgat tgttgttgcc gtcaaatcac
      481 cgctctcctg tttgaaatcc gctatgaaat gcaaaactct ataattacaa aaatttaaca
      541 aaaaacaaaa cttcaaacaa ctactatttt tttagcggtc gggcgagccg ccgccaaaat
      601 aaattataaa taaaatctct taaaattctt ccgctctggc ataagcattg ctaaatcatc
      661 tttcgatgaa atgagttttt catcgcaact ttagtaaagc ttcgataaac attgataaat
      721 cgtgaaaagg cgttttccct gattgtcggg aaatgccgta acttgtcaca gaaaatgaca
      781 aaaattgtca gccgcacggg ttttacgtta cttacacaag aggtatctat gaacaataaa
      841 aagcagaagg gtttttcttt aatcgaatta ttaatagtga tggtcattct cggaatcctg
      901 atgattctga catttccata cgttcgcaaa gccaaagacg ccgccgaaga cggaaacgcg
      961 tttgcgtcga tgaagacgat gctcgccgca cagtataatt tttattctca aaacgccaga
     1021 tacgccgctt tgacggaatt aaacgattcg cagtacggct cgctcggaat aaacgccggc
     1081 acctctctga gacgcggaca atacacgttt gttatgagcc ccgtcacccc gaccccgacc
     1141 gagcttcgtg aaggatttac gattatcgcg accagacccg gctacggcac cgagccgccg
     1201 gtcacgctga ccctgactga aagaggacac gccgatttta tttttgatta gattagagcg
     1261 gctcgtctgc cgccggcgct cgccaaatct attcagatgt tttactcaaa aaaaatccaa
     1321 gggaaagaat acatgaacgt ttttgaagac cttgtcgaag aactaaaaga agaaaatttg
     1381 cttgaagaaa ccgtcatcgg aagaaacgcg gcgcaaactc acgccgtgga atatgtcgcc
     1441 gacgcttcac aatttcaggc gcaaaccggc gaaacgccgg ctgaattcga atcgacgccg
     1501 agcttttcgg cggatttcgc atttgagccg gatgagcccg aagcggaaga attatttccg
     1561 gaagagtttt taagcgaaga aaaatcatcg gattttttat ttgccgggca cgaacgaagc
     1621 gaaaataaga taagcgaatc gagctttgaa acagccgcgc ccgagccgga aaattttgct
     1681 gcgccgaaag cgcaggtcga cgaggcggag ttttatcgtc gccgggcgat tgacgaagtg
     1741 acttcgctgc aaatggtcga gcacgttttt tcgggcgttg agcgcgagca gatgcgcatt
     1801 ctgcccaaac ctttcaacga cattacggtc aaaaaagccc tgcacgattt tttgcaggtt
     1861 tccaaaaatt cgcccggcaa cgaaaacgct caagccgaat tcaaattaat gcaggagacc
     1921 gaaagctggt gctcggcgct gtcgcatcgc gataagcatc tttcggtcag cgctctgcgc
     1981 cgctactgcg aaacgacgaa accttcctta agcgaccagg caatcatcgc gctggcgcgt
     2041 ttttaccgga atctgccgca caccgaatcg gtcagaagca aattcgatat gattctgacg
     2101 cgtctgttta caagagaggt cgaaggcgat agacgcgagc tgcagtttga ccgcgacgac
     2161 atggtcaggc agattagcga gctttacgcc gattgggcga gcgtgccgct ttattcggtc
     2221 gaagacgaaa ccgaattggt gctcatcacc gtcaaattcg acgattttct cgccgaggcg
     2281 caggacgcgg aaaatttcga cgagctggtc aaaaaggatt tttacaatcg tctgcggctt
     2341 tttaaagaga gcatcagcga taaattctac gcgccgctgg ttctggcttc ggcagtcgaa
     2401 agcaatgttt taatcggcaa tcgctacatc gagctgctcg agaaagagcg ccggcacacg
     2461 agcgccgaag cgattgaaag ccagtatagc tttctgctcg accaggctat ttccgacgcg
     2521 accagcaaaa cgctgcaatt ggtcgaactg ctcaaggaaa aaaccgaagt cgtcgtcgtc
     2581 gagccgaaga cgctcgacga gccgaaagag actctcgccg ttcccgaaat aaaagtcgag
     2641 cgtaaaaaaa ccaggcaatc gcgcgtcggc tttttcggta tcagtcaatg gctggcgacg
     2701 gctctggtca gcgtgattct gctcagcgcc gcgctttatt tttacgtcga atatttcagc
     2761 acgcgccgcg ccgcgccgtc cgtgcagaaa gtaaatatag aaaactccgc ctttaaagat
     2821 tacctgcaga cggcgcggat taatcaggaa acctttatcg gcgtcgtcaa tccggcttgg
     2881 gaagtgataa acgaagataa aaagcaggaa attctcgaca aactgatgaa cgtcggcagg
     2941 gaaaaaggct ttaccaaagt tcatctgctc aacaatcagg gcgaaacggt cggctacgcg
     3001 accgaagccg aagcaaaggt tattaatccc ggctcgattt agactccata gagttaagtc
     3061 tcgaacgaaa gcgaattcag ccgcgcgaga acaggaattc tttcgcggct gaattttgtt
     3121 cggcggtttt gcgtttcggc aggtttgttg taaactctct gagtattttg cagcgaactt
     3181 gccgaaaatt tccttcaaga ctaattctat aaagtcaatt taaaaggaaa ttatgaacgc
     3241 aaaaaatgtc ttgaactacg ccgccgacca gggcgctaaa tttgtttccg tgcgttttac
     3301 ggatttgccc ggcgcgtggc atcacttaac ttacccgatt gggcagctga ccgaagattc
     3361 atttgaagac ggtttcggtt tcgacgcctc gtctctgaga ggctgggcgg cgataaacga
     3421 atccgatatg ctgctcgtgc ccgacccttc gcgctgctgg gttgacccgt tcgccgagga
     3481 aacgaccgtc tgtttaatcg ccaacgtggt tgacccgata acgaaggaag gttacgggct
     3541 cgacccgcgc tcggtcgccc tgcgcgccga aaattatctg aaatttacgg gcatcgccga
     3601 cacggtttat ttcggaccgg aagccgagtt tttcgttttc gacgaagttt tttatcacaa
     3661 caaccagaat tcagccggct tttccgttaa ttcggaagaa ggacactgga attcggggcg
     3721 cagcggcaac gatatgatgc cgaatatggg ctatcacatt cgaccgaagg aaggctacgt
     3781 gccggtcgcg ccgctcgaca ccctgattga tttgcgcaac gcgattgcgc tcgttttgga
     3841 aaacgtcggc atccacgtcg aatgtcatca ccacgaagtc gccaccgccg gacaatgcga
     3901 aatcgatttt aaatactcga acctgctgca caccgccgat aatttgatga tgttcaaata
     3961 cgtcgtcaaa aatacggcgt atcaatacgg caaaaccgcg acctttatgc cgaagccgct
     4021 ctacggcgac aacggttcgg gaatgcactg tcatcaatct ttgtggaaag acggcaaccc
     4081 gcttttcagc ggcgaccaat acgccggact ttcggaaatg gcgaaattct acatcggcgg
     4141 cttgctcaaa cacgcgcccg caatcgtcgc cttcgccgcg ccgaccacca acagctacaa
     4201 acgcctcgtt ccgggcttcg aagcgccggt caatctggca tattcggcgc gcaatcgctc
     4261 ggctgccgtc cgcatcccga tgttttcgca atcgcccaaa gccaagcgcc tcgaatttcg
     4321 cccgcccgac ccggcgtgca atccgtatat caccttcgcc gcattgctga tggctggttt
     4381 ggacggcgtc cagaaccgca tcgacccggg cgagccgctc gataaagaca tctacgattt
     4441 gtcgcccgaa gaattaaaag acgtcccatc cttgccggga agtctggacg aagcgctcaa
     4501 ggctctggaa gacgaccacg agtttctgct caaaggcgac gttttctcga cctcgatgat
     4561 tgagcgctgg attacataca agcgcgaaaa agaaatccag ccgcttcgcc tgcgcccgca
     4621 cccgctggag ttttcgatgt attacgacat ttaagcctga acaaaatacg cttaaacccg
     4681 agaggaacaa tttttattgt tcctcttttt attttcttgt atatttagtg tcacgaaaaa
     4741 cttacaaatt aataataaag ctttgcaaaa cgttaatgac ggcgctgtca cgatcaatcg
     4801 gatatgagta atgaagcaaa aaatgaagga gacgggaatt tttttattca aggtatttcc
     4861 ggctccgaga taactatcaa tcccaaacat tctcatagcg ataaaaagcc tgcacctctt
     4921 ccaaaacaca tcacaatcca atctctaact ccgccgaatt attttacggg tcgcgaaaaa
     4981 gttctaagcg atattgccga aactttgaat gctcgtggaa aagctgcttt gtgcggaatt
     5041 tcggggcttg gaaaatcgtc ggtggtttta aaata
//
LOCUS       DASWNE010000190         2223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_288507, whole genome shotgun sequence.
ACCESSION   DASWNE010000190 DASWNE010000000
VERSION     DASWNE010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288507"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VGB68_03945"
     CDS             <1..371
                     /locus_tag="VGB68_03945"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048412.1"
                     /translation="LIQKARVAARNSDWNSRISSNEESNNPKIYLGAIAAGEKVIAST
                     EAEIFKFLKQNYNDTLAIEMEGRGFLTALHANESVQSIVLRGISDLLDNKSDSSDSNR
                     QEIASKNVSAFAFEMLSNIS"
     gene            complement(406..1560)
                     /locus_tag="VGB68_03950"
     CDS             complement(406..1560)
                     /locus_tag="VGB68_03950"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0048413.1"
                     /translation="MAWNKDTAKTEKEMSGAETFFVLSPRRRLIVSIIIAVSIVLVTA
                     LHFLTPVDQVLWHEIYQRLYYIPIISAALLFGLRGGLTASLFTTVVYSPHIYLHWQHG
                     HFDYSINQYAEIVIFNVVGGVMGALGDRLKQARIRAERNAEERQKAYDELQKTFEQLL
                     QAEKLGALGELSAGIVHEVRNPLASIKGAVEILEDELAPDSPRREFADLAKKEIERLD
                     KLVGEFLRFARPGALSVEKNDLNKIVESVASLIENQAAAQRISVEKNLQKRLPEVSVD
                     AEQIKQVLLNLAINSLQAITKDSRDGGGKLVFRTFEKDGFCIAEISDTGGGIDEKILP
                     KIFDPFFTTKEKGVGLGLSIAHKIAVQHNGQLSAANRNGHTVFSLQLPKF"
     gene            complement(1547..>2223)
                     /locus_tag="VGB68_03955"
     CDS             complement(1547..>2223)
                     /locus_tag="VGB68_03955"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY0048414.1"
                     /translation="KFEEAQHGTLFLDEISSMPVALQSRLLRVLQEQEITRLGENTPR
                     KIDVRIIAATNENLTELIKQNEFREDLYYRLAVVPVKLPSLRERREDIPLLVEHFLKK
                     SADKYGIKQPKPEREVFNAFFNYPWMGNVRELENVIERMIVLSADGETLTLEDVPENI
                     KNPPEKLENLWFALPAEPINLENIECEIIREALKRYDGNQSQTAKYLGITRSALIYRM
                     QKYGLE"
BASE COUNT          546 a          549 c          539 g          589 t
ORIGIN      
        1 cactaattca aaaagcaaga gttgcagcta ggaattcaga ttggaattca agaatttctt
       61 ccaacgagga aagtaataat ccgaaaattt atctcggtgc gattgcagcc ggcgaaaagg
      121 tgattgcttc aacagaagca gaaattttta aatttttgaa gcaaaattat aatgacactt
      181 tagctataga aatggagggt cgaggatttt tgactgctct tcatgccaat gagtcagttc
      241 agtcaattgt cttgcgcggc atatctgact tattagacaa taaaagcgac agttctgatt
      301 caaatcgcca ggaaatagct tcaaaaaacg taagtgcttt tgcgtttgaa atgctgagca
      361 atatttcctg acaaccggta tacgcgctta aatttataca actaactaaa atttaggcaa
      421 ttgtaatgaa aaaaccgtat gaccgttccg gttcgccgcc gacaactgac cgttgtgctg
      481 cacggcaatt ttgtgagcga tggaaagtcc taatcccacg cctttttcct tggtggtaaa
      541 aaacgggtcg aaaatcttcg gcaggatttt ttcatcaatt ccaccgccgg tgtctgaaat
      601 ttcggcaatg caaaagccgt ccttttcaaa cgtccgaaaa acgagttttc ccccgccgtc
      661 ccgactgtct ttagttattg cctgcaagga attaatcgct aagttgagta gcacctgttt
      721 gatttgctcg gcatcgaccg agacttccgg caaacgcttt tgcagattct tttcgacgga
      781 aattctttgc gccgcggctt gattttcgat gagagaagcg accgattcga caattttatt
      841 caaatcattt ttttcgaccg aaagcgcgcc gggacgcgca aagcgcagaa actcgccgac
      901 cagcttatcc aatctttcga tttccttttt cgccaggtcg gcaaactccc ggcgcgggct
      961 gtccggcgca agctcatcct ccagaatttc gaccgcgcct ttgatagaag ctaagggatt
     1021 gcggacctcg tgaacgattc cggcggaaag ctcgcccaaa gcaccgagct tttcggcttg
     1081 caggagctgc tcgaaagttt tttgcagctc gtcgtatgcc ttttgacgct cttccgcatt
     1141 tctttcggca cgaatcctag cctgtttgag ccggtcgccg agcgcgccca tcacgccgcc
     1201 gacgacgttg aaaatcacga tttccgcgta ttgatttatc gagtagtcga aatgcccgtg
     1261 ctgccagtgc aggtagatat gcggcgagta aacgaccgtt gtaaaaagcg atgccgtaag
     1321 cccaccgcgc aaaccgaaaa gaagcgccgc cgagattatc gggatgtaat aaagacgctg
     1381 gtatatctcg tgccagagaa cctggtcaac cggcgtcaga aaatgcagag ccgtcacgag
     1441 gacgattgag acggcgatga tgatggaaac gataagccgt cggcgcggcg acaggacaaa
     1501 aaaggtttcc gcgcccgaca tctctttttc cgtctttgcc gtatctttat tccaagccat
     1561 atttctgcat ccggtagatc agcgcgctcc gggtaatgcc caggtatttt gccgtctgcg
     1621 attggtttcc gtcgtagcgc tttaaagcct cgcggataat ttcgcattct atattttcca
     1681 gatttatcgg ttcggcggga agcgcaaacc agagattttc cagtttttcc ggcggattct
     1741 tgatattttc cggcacgtct tcaagcgtca atgtttcgcc gtcagcgctg agaacgatca
     1801 ttctttcgat gacgttttcc agctcgcgca cgtttcccat ccacgggtaa ttgaaaaatg
     1861 cgttaaagac ctcgcgctcg ggcttcggtt gtttgatgcc gtatttatcg gcggactttt
     1921 tgagaaaatg ctcgaccaag agcggaatgt cctcgcgccg ttcgcgcaag gaaggcagtt
     1981 tcacggggac gaccgccagg cgataataca aatcttcccg gaactcgttt tgtttgatga
     2041 gctccgtcag attttcattg gtcgcggcaa tgatgcgaac gtctattttt cgcggcgtgt
     2101 tttcgccgag acgcgtaatt tcctgctcct gcaaaacgcg cagcagccgc gactgcaagg
     2161 cgacgggcat tgagctgatt tcgtccagaa acagcgtgcc gtgctgggct tcttcaaatt
     2221 tac
//
LOCUS       DASWNE010000191         2130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_288526, whole genome shotgun sequence.
ACCESSION   DASWNE010000191 DASWNE010000000
VERSION     DASWNE010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288526"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..1715
                     /gene="dacB"
                     /locus_tag="VGB68_03960"
     CDS             27..1715
                     /gene="dacB"
                     /locus_tag="VGB68_03960"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEY0048415.1"
                     /translation="MKYFSIAGIHLLAFFLLAFTFSGCYSNKPAEIPSQSDSNSSVVL
                     PDAGESAFEIPAPDINLANPLVVSQKPEDVAMCEKINRRIDESQAGNARWGVFAVSLK
                     DGRVVCSRDGRKLFNPASIQKTLTAIVALDKLGADFRWKTSVYSENQIEADGTLNGSL
                     TLYGEGAPDFDAAAMENLINQLQAKGLKKVKGNISGDESYFRGDTIGDGWTWNELQWY
                     YGAEASALSFNDNQAFINVENGAARTSTDYIQVTIGKPAAAESNTNAEASGIKRGLSD
                     NHFYVWGNAKNFGARVAVHNPAGLAAKNLKEALEKKGIAVEGEVRSRDWKADDKFDTA
                     KAQELASVESRTLGEIVRKMNKHSVNLYAELILRTIGKRFAADVPGDIQRPQNVRGDD
                     TAGAAIIKQWLRQRSVATGEIEVADGSGLSRLNFVTPESFGRAFIYAAQSPFAQAFTD
                     SLPIAATDGTLGGRLAKVKGKVLAKTGTVTFVNSLAGYADNAGGEVFAFAVVTNNDTR
                     KNGTIGVIDAVTTILVTGADEKETPKETPAANNNQNNKGNANANQLNFVIRNNK"
     gene            1726..>2130
                     /locus_tag="VGB68_03965"
     CDS             1726..>2130
                     /locus_tag="VGB68_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048416.1"
                     /translation="MTRSFFPLNYHLSDKDSEKKEKPEQNHHKQRDNRDEQTAEICPR
                     LLIPGVSFGAMRAFALACQKGTLAPVADFDFHALRISKITAKFNGFRTSKTDFFFPAR
                     ISRFFLAPGKNRLRKAYFLCANNIFRAQAVFSP"
BASE COUNT          547 a          542 c          592 g          449 t
ORIGIN      
        1 taaataaata tcaagctagt taatcaatga agtatttttc aattgccgga attcaccttt
       61 tggcattttt tctgctcgct ttcacttttt cgggctgtta ctcaaacaag ccggcggaaa
      121 ttccgtcgca gtccgattcc aattcgtccg tcgttttgcc tgacgccggt gaatccgctt
      181 ttgaaattcc cgcgccggac atcaatctcg ccaatccgct ggtcgtttcg caaaagccgg
      241 aagacgtggc gatgtgcgaa aaaatcaatc ggcggattga cgaaagccag gctggcaacg
      301 cgcgttgggg cgtttttgcc gtcagcttaa aagacgggcg cgtcgtctgc tcgcgcgacg
      361 ggcgcaagct ttttaatccg gcttcgattc agaaaacctt aacggcaatc gtcgcgctcg
      421 acaagctcgg cgcggatttt cgctggaaaa cttccgtcta ctccgaaaat cagattgagg
      481 cggacggcac gctgaacggc agtttgacgc tttacggcga aggcgcgccg gattttgacg
      541 ccgcggcgat ggaaaatctt atcaatcaat tgcaggcgaa aggtttgaaa aaagtaaaag
      601 gcaatatcag cggcgacgaa agttattttc gcggcgacac aatcggcgac ggctggacgt
      661 ggaacgagct gcaatggtat tacggcgcgg aagcctcggc gctgtcgttt aacgacaatc
      721 aggcttttat caatgtcgaa aacggcgcgg cgcgcacttc gaccgactat attcaggtga
      781 ccatcggcaa acccgccgct gccgaaagca atacgaacgc ggaagcttcc ggcatcaagc
      841 gcgggctttc cgacaatcat ttctacgtct ggggaaacgc gaaaaatttc ggcgcgcgcg
      901 tcgcagtgca taatccggct ggtctggcgg cgaaaaacct gaaagaagcg ctggagaaaa
      961 aaggcatcgc ggtcgagggc gaagtccgct cgcgcgattg gaaggcggac gataaattcg
     1021 atacggcgaa agctcaggaa ctggcgtcgg tcgaaagccg gacgctcggc gaaatcgtgc
     1081 ggaagatgaa caagcattcg gtaaatcttt acgccgagct gattttgaga accatcggga
     1141 aacggtttgc cgccgacgtg ccgggcgaca ttcagcgacc gcaaaacgtg cgcggcgacg
     1201 acacggcggg cgcggcaatc atcaaacaat ggcttcggca gcgcagcgtg gcgaccgggg
     1261 aaatagaggt cgcggacggt tcgggtttat cgcgcctcaa tttcgtcacg cccgaatctt
     1321 tcggcagagc ctttatttat gcggcgcaaa gcccgtttgc ccaggcgttc accgattcgc
     1381 tcccgattgc cgcgaccgac gggacgctgg gcgggcggct ggcaaaagtg aagggaaaag
     1441 tgctggcgaa aaccggtacg gtaacttttg tcaattcgct cgccggttac gccgacaacg
     1501 cgggcggcga agttttcgct ttcgccgtcg tcaccaacaa cgacacgcgc aaaaacggga
     1561 caatcggcgt catcgatgcc gtgacgacga ttctggtgac gggagcggac gaaaaggaaa
     1621 cgccgaaaga aacgccggcg gcgaataata atcaaaacaa taaagggaac gccaacgcga
     1681 accagctgaa ttttgtgatt agaaacaata agtgataagt gataagtgac gagaagtttt
     1741 tttcctctca actatcactt atctgataaa gattccgaga aaaaagaaaa gccagaacag
     1801 aatcatcaca aacagcggga taatcgtgat gagcagacag ccgagatttg cccacgactc
     1861 ctgattccag gtgtaagctt cggcgcaatg cgcgcattcg cccttgcttg ccagaaaggg
     1921 acgcttgcac cggtggcaga ttttgatttc cacgcgctta gaatatcaaa aattacagcg
     1981 aagtttaacg ggtttcggac ttcaaaaacg gattttttct ttccggcacg gataagccgg
     2041 tttttccttg cgcccggcaa aaaccgtctg cgcaaggcat attttctctg cgcaaacaat
     2101 atttttcggg cgcaagccgt attttctccg
//
LOCUS       DASWNE010000192         2555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_253511, whole genome shotgun sequence.
ACCESSION   DASWNE010000192 DASWNE010000000
VERSION     DASWNE010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253511"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VGB68_03970"
     CDS             complement(<1..313)
                     /locus_tag="VGB68_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048417.1"
                     /translation="MSELKKTQKTSTLVFVLVFAFSLSLSARAQVLDSPYIYSNPALQ
                     QDAFVRNMNAIAIEGLMQRHMIEQSVGGKRAQSPAGANRSSTLFRGSKQFVIADLLVK
                     QS"
     gene            complement(433..660)
                     /locus_tag="VGB68_03975"
     CDS             complement(433..660)
                     /locus_tag="VGB68_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048418.1"
                     /translation="MATNPIGNFNPNELTPDQRKTYEMQQELMRQQANGSMMTLMFQH
                     QQQQQKERFDSLSNTLKSGSDTRGNMINNSK"
     gene            complement(680..1156)
                     /locus_tag="VGB68_03980"
     CDS             complement(680..1156)
                     /locus_tag="VGB68_03980"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0048419.1"
                     /translation="MLKAQETIREKLKISPEEFSEMGRVGAMFYQQGNLDKARTIFEG
                     LVELDPHSADAHSALGALLTLQQEDEPAVEHLRKAVELNPRQIAPYVNLGEVYIRQQK
                     LEEAVASLKKAIELDPDEKDSGANRARAMVLGIYQVIKAGNNPITDDTILDSETVN"
     gene            complement(1174..1620)
                     /locus_tag="VGB68_03985"
     CDS             complement(1174..1620)
                     /locus_tag="VGB68_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048420.1"
                     /translation="MFGIGKLLGSVAGGLLDKIGLGKIAPFVQLGINALTGNWLGVAK
                     DVFGLVSNFKGNFLNNASTRPPLGGFEQNNFAPDQNNRLSRNRISDLFRGLSNLFRGF
                     KSLGNGDFAGGFGRIFSAFNVLRDAFDNNQMFNNRSSASIFANIRL"
     gene            1869..2348
                     /locus_tag="VGB68_03990"
     CDS             1869..2348
                     /locus_tag="VGB68_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048421.1"
                     /translation="MEKRLSKMQFEHEWINGNISLGAAAGWTADEIRLVSELGYALAQ
                     QGRNYEAITIFEGLIALAPATTYFEATLGALWLRENNPARALPHLNVALNADPNDIPT
                     RVNRGEVFLRLENYEAAREDLHFALSRKIVPEKQSLLAQCLTRARALLYTVERFSRT"
BASE COUNT          613 a          640 c          609 g          693 t
ORIGIN      
        1 tcgattgttt gaccagcaaa tcggcgatga caaattgctt tgagcctctg aataaagtcg
       61 aagaacggtt agcgcccgcg ggcgattgag cgcgctttcc gccgacgctc tgttcaatca
      121 tatgtcgctg catcaagcct tcaatcgcaa tggcgttcat atttcgcacg aaagcgtcct
      181 gctgcaaagc cggattgctg taaatatacg gcgaatccaa aacctgcgcc cgcgccgaga
      241 gcgaaaggga aaatgcgaaa acgagaacga aaacgagcgt cgatgttttt tgggtttttt
      301 tgagttcaga catatttttc aatctccaaa ccgattataa aacaaagaaa gtcgaaaccg
      361 ccgcacaaat gtgagcgtat tcgactttct cgaaagaaac ttttgccgga ttaatactct
      421 gcctaaccga gattatttag agttgttaat catattgccg cgcgtatcgc tgccggattt
      481 aagcgtgttg ctcagcgagt cgaagcgttc tttctgctgc tgctgttgat gttgaaacat
      541 caaggtcatc atgcttccgt tcgcctgctg ccgcatcaat tcctgctgca tttcatatgt
      601 tttgcgctgg tcgggtgtta attcgttagg gttaaagttg ccgataggat tcgttgccat
      661 tgtttttttt ctccttcttt taatttactg tttctgaatc taaaattgtg tcatccgtta
      721 ttggattatt gcctgcctta ataacctgat agattccgag caccatcgcg cgggcgcggt
      781 tcgcgccgga atccttttcg tccgggtcga gctcgatggc ttttttcaag ctcgccaccg
      841 cctcttccag tttctgctgc cggatgtaaa cttcgccgag gtttacatac ggagcgattt
      901 gtcgagggtt gagttcaacc gcctttctca gatgctcgac cgccggctca tcttcctgtt
      961 gcaatgtgag cagcgcgccg agagccgaat gcgcgtcagc gctatgcggg tctaactcaa
     1021 ccaaaccttc aaatatcgtt cgtgccttat ccagattgcc ctgctggtag aacatcgcgc
     1081 cgacgcgtcc catctcgctg aactcttcgg gcgagatttt caacttctcg cggattgttt
     1141 cctgtgcttt cagcataatt caccgtttaa aaactacagt cgaatattcg cgaatatcga
     1201 agccgacgag cggttattaa acatctgatt gttgtcaaaa gcgtcacgca gcacgttgaa
     1261 cgccgaaaat attctgccga agccgcccgc gaaatcgccg ttgccgagcg atttgaaacc
     1321 tctgaacaag ttgctcaagc ctctgaacaa atccgaaatc cgattgcggc tgagccgatt
     1381 gttctggtcg ggagcgaaat tgttttgctc aaagcctccg agcggcgggc gcgtcgaagc
     1441 gttgttcaga aaattgcctt taaaattgga aaccaatccg aaaacgtctt ttgcgacgcc
     1501 gagccagttt ccggtcaggg cgttaatgcc gagctggaca aacggcgcga tttttccaag
     1561 cccgattttg tctagcagac cgcccgcgac gctgcctaat aatttaccga ttccgaacat
     1621 ctttccattc ctcctgctat tgctttatgc caatgcgctg aactaacctt gatttgcgtt
     1681 tttcaattgt ctgtgtaagt tatcggcgcg gctgcggaaa agtttcctgt taatcgtaaa
     1741 ttttttttga gaatttttgc gagtttcaaa tagggattat tttccgccgt catcagatta
     1801 gaacgccgat tgatgccgca attaaatttc tccgattgaa aatgacttta aaaagcattt
     1861 aagctaaatt ggaaaagcgg ttatcgaaaa tgcagtttga acacgaatgg ataaacggaa
     1921 acatctcgct cggcgcggcg gcgggctgga cggcggacga gattcgtctg gtttccgagc
     1981 tcggctacgc gctggcgcag cagggtcgca actacgaggc gattacgatt tttgaaggct
     2041 taatcgcgct cgccccggcg accacttatt ttgaagccac gctcggcgcg ctctggctcc
     2101 gggaaaacaa tccggcgcgc gctttgccgc atttgaacgt cgcgctcaac gccgacccga
     2161 acgacattcc gacccgcgtc aatcgcggcg aagtctttct gcgtctggaa aattacgaag
     2221 cggcgcgcga agatttgcac ttcgcgctct cgcgcaaaat cgtccccgaa aaacaaagtc
     2281 tgctcgccca gtgcctgacg cgcgcccgcg ctctgcttta cacggtcgaa agattttcaa
     2341 ggacgtaaaa agtggtaagc ggtaagctcg ttgttttgag cggcgagctt taaataaaaa
     2401 aatcgatact ttaaaactgc aactgaccgc ttggcgttga ccgcttaccg ctaaaaaatt
     2461 gccgctccaa aattttttca tatattaagg aaaaaacagg aaactttttg cggaatctgc
     2521 cgataacttt ggataagcca taattctatt ttgga
//
LOCUS       DASWNE010000193         1585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_358260, whole genome shotgun sequence.
ACCESSION   DASWNE010000193 DASWNE010000000
VERSION     DASWNE010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358260"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..1008
                     /locus_tag="VGB68_03995"
     CDS             157..1008
                     /locus_tag="VGB68_03995"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0048422.1"
                     /translation="MKKLAILFLIIFSCSGAFAQSDLQKLYDTEKAFERAAAEKGVNQ
                     AFVEFSAPDGVCFNPEALNCREFWKSRPASPAGLFWNPNFVDVSSNGALGYTTGNSIY
                     RPKGKDDPDAYYGQYVTMWQRQPNGTYLAALDLGISHEKPATVETAWKSPADSGREPN
                     EKKISAADASTAFFETATNQGLGKAYKAFLAEDARLLRDGKQPILGKQNALAEFKNDK
                     TRIFFAKRSVFVGAADMAYISNSYTIGDKSGKQIEKGNFLQIWKLRGGKWQIVLDAFL
                     PIPEPKN"
     gene            1189..>1585
                     /locus_tag="VGB68_04000"
     CDS             1189..>1585
                     /locus_tag="VGB68_04000"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY0048423.1"
                     /translation="MSKTFSQVVELLKLEAAAIECAAKNLSEEAVEQAVRLLLECESK
                     AVVIGVGKSGIIANKIADTMTSTGTVAVFLHPSDALHGGLGVITRGDCVIALSNSGET
                     DEIIALLPALKQRGVSIIAIVGNLNSALAR"
BASE COUNT          416 a          390 c          412 g          367 t
ORIGIN      
        1 cggacacgtt atagctttca tatgtaaaat ccaagactta atttcagaca gatgacggaa
       61 tccgatagac attttgcgag aaattaataa ttattggagt tcgggcaatc ggagcgtcga
      121 aaaaaatgaa attcagtcaa aggaatcgtt ttgtttatga aaaaactcgc cattttgttt
      181 ttgattattt ttagctgctc aggcgctttt gcgcagagcg atttgcaaaa gctttacgac
      241 acggaaaaag ccttcgagcg cgccgccgcc gaaaaaggcg tcaatcaggc gtttgtcgaa
      301 tttagcgcgc cggacggcgt ttgttttaat ccggaggcat taaactgccg cgaattctgg
      361 aaatcgcgtc cggcttcgcc cgccggattg ttctggaatc cgaattttgt cgacgtttcg
      421 tcaaacggcg cgctcggcta cacgaccgga aactcgattt accgaccgaa aggcaaagac
      481 gaccccgacg cttattacgg tcaatacgtg acgatgtggc agcgccagcc gaacggcaca
      541 tatttagccg cgctggattt aggcatctcg cacgaaaaac ccgcgacggt cgaaaccgcc
      601 tggaaatcgc cggcggattc gggcagagaa ccgaacgaaa agaaaatttc cgccgccgac
      661 gcctcgaccg cgtttttcga gacggcgacg aatcaaggtc tgggcaaagc ctacaaagcg
      721 tttctggctg aagacgcgcg gcttttgcgc gacgggaaac agccgatttt aggcaagcaa
      781 aacgcgctcg ccgaattcaa aaacgacaaa acgcgaatct ttttcgccaa gcggtcggtt
      841 ttcgtcggcg cggctgatat ggcttatatc agcaattcct acacaatcgg agacaaaagc
      901 ggcaaacaga ttgaaaaggg aaattttctg caaatctgga agctgcgcgg cggcaaatgg
      961 caaatcgtgc tggacgcttt tctgccgatt cccgaaccga aaaattaacg gcaattcaaa
     1021 caggatggac aggataaaca ggattaatta agaatatgag cgtagagttt cgttctttaa
     1081 tcctgactat cctgtgcatc ctgtttgaat ctgcgtttat ctgcgaaaat ctgtggtttc
     1141 attcgtcttt ctttgtgttc ttcgtgtctt tgtggtgaaa ataaaaaagt gagcaaaacc
     1201 ttttcacaag tcgtcgagct tttaaagctc gaagccgccg ccatcgaatg cgcggcgaaa
     1261 aatctgagtg aagaagccgt cgagcaagcc gtccggctgc tgctcgaatg cgaaagcaaa
     1321 gcggtcgtca tcggcgtcgg caaatccggc attatcgcca acaaaatcgc cgacacgatg
     1381 acctcgaccg gcacggtcgc cgtttttctg catccgtcgg acgcgctgca cggcgggctc
     1441 ggcgtgatta cgcgcggcga ttgcgtgatc gccttgagta attcgggcga gaccgacgaa
     1501 atcatcgcgc tgcttccagc cctgaaacag cgcggagtct cgataatcgc gattgtcgga
     1561 aatttgaatt ccgctctggc gcgta
//
LOCUS       DASWNE010000194         2759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_393071, whole genome shotgun sequence.
ACCESSION   DASWNE010000194 DASWNE010000000
VERSION     DASWNE010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393071"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VGB68_04005"
     CDS             <1..396
                     /locus_tag="VGB68_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048424.1"
                     /translation="QVMLFYILRRVPENNKKVNPQSPDAMVEEMLKAVAKTPGFEGLP
                     KTVAEFEASVKKHLPGVADWRKIDQKIFDPNPDLGLFTNDIADASVNFRGKFMVRLLT
                     AEVEKGERVFAVVGASHVVKQERALRKLF"
     gene            complement(430..>2759)
                     /locus_tag="VGB68_04010"
     CDS             complement(430..>2759)
                     /locus_tag="VGB68_04010"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0048425.1"
                     /translation="YDGGTTEDGSPYLIMEYVEGETLLEYCDENRLTVRERLELFEQI
                     CAAVEYAHRQSVLHRDIKPSNILVKPGGTAKLLDFGIAKLDGDELSSQTKEITATGRW
                     MMTPEYASPEQVRGEGLTAAGDVYSLGVVLYKLVTGQPPYKFPSRAPHEIARVICEQE
                     VESPKPGAQSPNGDKLGSHLESIILKALRKRADERFQAVAALAAEIKNYLAGDAVSAE
                     IVPQSYDELSIDKPSQNSGSTLKSLAVLPLRQIHAGQPDAESEFLGVGLADALTTRLS
                     NVRQIIVRPTSSVVQFAQKSDDSLALGKQLAVDYVLEGRVTRNDSQIRVIVQLLKIND
                     NSVLWASQFDESEVDIFALQDSISEKVVASLAPHLTEAEAARLNLRGTQNAAAYEAYL
                     RGRFHLLTYEAGGITGSIRYFQEAARLDPNFALAHAGIAEYYNWMEVYSFLRPNECWA
                     AAKTAARRAVELDPLLSEAYAALAFTVWGHDWDFAESERLYRRAVELNPNNPRAHEWF
                     SFLLQTVGRNDEAIAEMKIAQRLDPRSASHCAAHHYVLHNARRYREAEEMLRRGLAID
                     PENILCNHGFGWSSPFLGQTEKGIEAARRAVESTNRVSIYLTTLGYDLAIGDRREEAR
                     KILREMDEREKRGEYFPQIYFAVIHAGLGETDAAFARLDKSAAERDHWMTTLLFDPRL
                     DSLRADSRFADYVKKIRPLHQPENKLPLPAQTKKTQSIETSEDKKNAAETQNSPIASR
                     SSEGVTLSWRLIAVVAISIAAILAIVLIYIIADAM"
BASE COUNT          573 a          781 c          792 g          613 t
ORIGIN      
        1 caggtgatgc ttttctatat cctgcgccgc gtgccggaaa ataataaaaa agtgaatccg
       61 cagtcgcccg acgcaatggt cgaagaaatg ttgaaagcgg tggcgaaaac gcccggcttc
      121 gaaggcttgc cgaaaaccgt cgcggagttt gaagcgagcg tcaaaaagca tctgccgggc
      181 gttgccgact ggcgaaagat tgaccagaaa attttcgacc cgaatcccga tttgggatta
      241 tttaccaacg acatcgccga tgcttccgtt aattttcgcg gaaagtttat ggtcaggctt
      301 ttaacagctg aagtcgaaaa aggcgagaga gttttcgccg tcgtcggcgc gagccacgtc
      361 gtcaaacagg agagagcgct ccggaaacta ttctgaggaa ttgcctgcgg tcaagcggtc
      421 ggttccggct cacatcgcgt cggcaattat ataaataaga acgattgcca gaattgcggc
      481 gatactgatg gcgacaacgg caatcaatcg ccaggacaaa gttacgcctt ccgagctgcg
      541 gctggcaatc ggcgaatttt gagtctccgc tgcgtttttt ttatcctcgc tcgtttcgat
      601 ggactgagtt tttttcgtct gcgccggcag cggcagttta ttttccggtt ggtgaagcgg
      661 gcggattttt ttgacgtaat cggcgaaacg cgaatcggcg cgcaagctgt cgaggcgcgg
      721 gtcgaacaaa agcgtcgtca tccagtggtc gcgttccgcc gcgcttttat cgagcctcgc
      781 aaacgccgcg tccgtttcgc cgagcccggc gtgaatgacg gcaaaataaa tctgcggaaa
      841 atattcgccg cgcttttcgc gctcgtccat ctcgcgcagg atttttcgcg cttcttcgcg
      901 ccggtcgccg attgccaaat cgtagccgag cgtcgtcagg taaatcgata cgcggttggt
      961 gctttcgacc gcgcgccgag ccgcttcgat gcctttttcc gtctgaccga gaaacggcga
     1021 agaccagccg aagccgtgat tgcacagaat attttccggg tcgattgcca gcccgcgccg
     1081 cagcatttct tcggcttcgc ggtaacgccg cgcgttgtgc agaacgtaat gatgcgccgc
     1141 gcagtgcgac gccgagcgcg ggtcgaggcg ctgggcgatt ttcatttccg cgatggcttc
     1201 gtcgttgcgc ccgacggttt gcagcagaaa cgaaaaccat tcgtgcgcgc gcggattgtt
     1261 cggattcagc tcgacggcgc gccggtaaag ccgctcgctt tcggcaaaat cccaatcgtg
     1321 tccccagact gtaaacgcca gcgccgcata ggcttccgac aagagcgggt cgagctcgac
     1381 ggcgcggcgc gccgccgttt ttgccgccgc ccagcattcg ttcgggcgca ggaatgaata
     1441 aacttccatc cagttgtaat attcggcgat tccggcgtga gcgagcgcga aattcgggtc
     1501 aagccgcgcc gcttcctgaa aatagcgaat cgacccggtg attccgcccg cttcataggt
     1561 gagcagatga aaacgtccgc gcagataggc ttcgtaagcc gccgcgtttt gcgtgccgcg
     1621 caggttaagg cgcgcggctt cggcttcggt cagatgcggc gcgagcgagg cgacgacctt
     1681 ttcggaaatc gaatcctgca gggcgaaaat atcgacttcc gattcgtcaa actgcgaagc
     1741 ccacaaaacc gagttatcat tgatttttaa aagctgtacg ataacccgaa tctgcgaatc
     1801 attgcgtgtc acgcgtcctt ccagaacgta atcaaccgcc agctgtttgc cgagcgcgag
     1861 cgaatcatcg cttttctgcg caaactgcac gacggagctg gtcgggcgca cgatgatttg
     1921 ccgcacgttt gaaagccgcg tcgtcagcgc gtcagccagc ccgacgccta aaaactccga
     1981 ttccgcatcc ggctgcccgg cgtgaatctg ccggagcggc aaaacggcga gagatttcag
     2041 ggtcgagccg gaattttgcg acggcttatc aatcgaaagc tcgtcgtaac tctgcggaac
     2101 gatttcagcc gaaaccgcgt cgcccgccag ataatttttg atttccgccg ccagcgcggc
     2161 gaccgcctga aaacgctcgt cagcgcgttt tcgcaaggct ttcaaaataa tcgattccaa
     2221 atgcgagccg agtttatcgc cgttcggact ttgcgctccg ggcttcgggc tttcgacttc
     2281 ctgctcgcag attacgcgcg cgatttcgtg cggcgcgcgg ctcggaaatt tatacggcgg
     2341 ctgtccggtg acgagcttat acagaacgac gccgagcgaa taaacgtcgc cggcggcggt
     2401 caagccctcg ccgcgcacct gttcgggcga ggcgtattcc ggcgtcatca tccatctacc
     2461 ggtcgcggta atttctttcg tttgagaaga tagctcgtcg ccgtcgagct tggcgatgcc
     2521 gaaatcgagc agtttcgccg tcccgcccgg tttaaccaga atgtttgaag gcttgatgtc
     2581 gcgatgcaga acgctctgac gatgcgcgta ttcgaccgcc gcgcagattt gttcgaacag
     2641 ctcaaggcgc tctctgacgg tcaggcggtt ttcgtcgcag tattcgagca aagtttcgcc
     2701 ttcgacgtat tccattatca aatacggcga gccgtcttcg gtcgtgccgc cgtcgtaaa
//
LOCUS       DASWNE010000195         3209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_146907, whole genome shotgun sequence.
ACCESSION   DASWNE010000195 DASWNE010000000
VERSION     DASWNE010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146907"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="VGB68_04015"
     CDS             <1..887
                     /locus_tag="VGB68_04015"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY0048426.1"
                     /translation="LSIIYLIVLLIQLFFLSNKLKHLLVERVLGRTKLDLGARQAIGT
                     ITRYLILFIGFLIILQTVGINLTTLNVLAGAVGIGVGFGLQNIASNFISGLIILFERP
                     VQVGDRIEIDDVNGKVISIGARSTIVRTNDNITIIVPNSKFISENVINWSFEGKVRFR
                     VPVGVGYNADINLAKKLLLRVAEENGDVLKEPKPSVRLIKFGESSIDLELWVWSKEKL
                     QRRAAFISDLNFAIWGKFRANNVEIPFPQTDLHIRSGKLKVENGEIAFSGGDETEENS
                     ENETSKESENETSKENEK"
     gene            877..1752
                     /locus_tag="VGB68_04020"
     CDS             877..1752
                     /locus_tag="VGB68_04020"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0048427.1"
                     /translation="MKNNAKQLLIIAVFTLLFAANAFAQNSAPQEKTATVFGAKIQYT
                     EAGDATKPAVVLLHGLGSSSVAWQFNIAPLAAKYRVIVPDQIGFGKSDKPMLKYRVGT
                     YVDFLDKFLSEIKVEKATLVGNSLGGWVAAWTAIKYPNRVEKIVLADAAGLKPNEVDF
                     NLIYSLNYSTRDEVRQLVKLVFYNQAIFGSEMFIDQSMSVRVQANDGYTINSLIESIK
                     RDEDFLDGQLAKIKKPTLIIWGKQDGLLKLADGERFNREIAGSRLVVFDQCGHVPQVE
                     KAADFNAEVLRFLEK"
     gene            1859..2260
                     /locus_tag="VGB68_04025"
     CDS             1859..2260
                     /locus_tag="VGB68_04025"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY0048428.1"
                     /translation="MQLKIGDTFSSSKQITDAVVRAFAELSGDYNPIHLDDEFAAKTR
                     FGKRIAHGMISGALISAVLGYELKDRKIVYLSQTMKFTAPVFIDDTVTATATVTNIRE
                     DKNIVTVETICTNQNGETVVKGEAAIMVLPD"
     gene            2265..>3209
                     /locus_tag="VGB68_04030"
     CDS             2265..>3209
                     /locus_tag="VGB68_04030"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0048429.1"
                     /translation="MQENFPSEAPPKATSYAWYALALLTLVYTLNFLDRQIIYILFQP
                     IKQEMSLSDTQLALLGATSFVIFYTLLGIPFGWLADKGSRKMLIGVGLVVWSVFSGLT
                     GFAADFWTLFFCRLMVGVGEATLGPAAISLLSDYFPPTKRATVTSIYSMGIAVGAGLA
                     AFLGGWIGLNYGWRWAFYLLGFPGIVIALLVFLLREPARSQDAKQTGAEYSRADWKNL
                     LTGKPFILLCLGYAFLGLATNNLSIWGAVFYTRVFGLNIATIGFYTGIFVIIAGIPAT
                     LYGGVLADWFRRKSRGGRMLYGAILCVISVPFWLVLLFA"
BASE COUNT          748 a          773 c          871 g          817 t
ORIGIN      
        1 ctctgagcat catttatcta atcgttctgc tgattcagct tttctttttg tcgaataaat
       61 taaagcatct tttggtcgag cgcgtgctcg ggcggacaaa gctggatttg ggagcgcggc
      121 aggctatcgg cacgataacg cgttatctta ttttgtttat cggttttttg ataattctgc
      181 aaacggtcgg catcaatttg acgacgctca acgttctggc gggcgcggtc ggcatcggcg
      241 tcgggttcgg cttgcagaat atcgccagca attttatcag cggcttgata attttattcg
      301 agcgcccggt gcaggtcggc gaccgcatcg agattgacga cgttaacgga aaagtgattt
      361 ccatcggcgc gcgcagcacg attgtgcgga caaacgacaa catcacgatt atcgtgccga
      421 acagcaaatt tatttccgaa aacgtcatca actggagctt tgaaggcaag gtgcgttttc
      481 gcgtgccggt cggcgtcggc tacaacgccg atattaatct ggcgaaaaag ctgcttttgc
      541 gagtggctga ggaaaacggc gacgttttga aagagccgaa gccgtccgtt cggctgatta
      601 aattcggcga aagctcgatt gatttggagc tctgggtttg gagcaaggaa aaattgcagc
      661 gcagagccgc tttcatcagc gatttgaatt ttgccatctg gggaaaattc cgcgccaaca
      721 atgtcgagat tccgtttccg caaaccgatt tgcacattcg cagcgggaaa ctgaaagtcg
      781 aaaacggcga gattgctttt agcggaggcg acgagaccga ggaaaattct gaaaatgaaa
      841 cgagtaaaga atctgaaaat gaaacgagta aagaaaatga aaaataatgc gaaacaatta
      901 ttgataattg ccgtttttac gctgctgttt gcggcaaacg cttttgcgca aaactctgcg
      961 ccgcaggaaa aaacggcgac ggttttcggc gcgaagattc aatacacgga agccggagat
     1021 gcgacgaaac cggcggtcgt tttgctgcac gggctcggct ccagctccgt cgcctggcag
     1081 tttaacatcg cgccgctggc ggcgaaatat cgcgtcatcg tgccggacca aatcggtttc
     1141 ggcaaatccg acaaaccgat gctcaaatac cgcgtcggca cgtatgtcga ttttctcgac
     1201 aagtttttat cggagataaa agtcgaaaaa gcgacactcg tcggcaattc gctcggcggc
     1261 tgggtcgccg cgtggacggc gattaaatat ccgaatcgcg tcgagaaaat cgttttggcg
     1321 gacgccgccg gattaaagcc gaacgaagtc gatttcaatt tgatttattc gctgaattac
     1381 tcgacgcgcg acgaagtccg gcagctcgtc aagctggttt tctacaatca ggcgattttc
     1441 ggcagcgaga tgtttatcga ccagtcaatg tccgtgcgcg tgcaggcaaa cgacggctat
     1501 acgataaaca gcctgattga atcaatcaaa cgcgacgagg attttctcga cggtcagctc
     1561 gcaaaaatca aaaagccgac tttgataatc tggggcaaac aggacggttt attaaaactc
     1621 gccgacggcg agcgtttcaa cagggaaatc gctggctcgc ggctcgtcgt cttcgaccag
     1681 tgcggtcacg tcccgcaggt cgaaaaagcg gctgatttta acgctgaggt cttgaggttt
     1741 ttagaaaaat aaattcggtc ttcggctttt ggtcttcggt cttcgatttt ttactgttca
     1801 acattgagca tattctttaa cgaagacaag cccgaagccc gaagaccgat aaaaaattat
     1861 gcaattaaaa atcggagata cattctccag cagtaaacaa attaccgacg cggtcgtgcg
     1921 ggcttttgcc gagctttcgg gcgattacaa cccgattcac ctggacgacg aattcgccgc
     1981 caaaacgcgt ttcggaaaac gaatcgcgca cgggatgatt tccggcgcgt tgatttcagc
     2041 cgttctcggc tacgaattga aagacagaaa aatcgtctat ctttcgcaga ctatgaaatt
     2101 caccgcgccg gtttttatcg acgacacggt gacggcgacg gcgaccgtca caaacattcg
     2161 cgaagataaa aatattgtca cggtcgagac gatttgcacg aatcaaaacg gcgaaacggt
     2221 cgtcaaaggc gaagcggcga taatggtttt gcccgactag aattatgcag gaaaattttc
     2281 catccgaagc gccgccgaaa gcgacgtcgt atgcgtggta tgcgctcgct ttattgacgc
     2341 tcgtctacac gctgaatttt ctcgaccggc agataatcta cattcttttt cagccgataa
     2401 agcaggaaat gtctttgagc gacacccagc ttgcgcttct gggcgcgact tcattcgtta
     2461 ttttttacac tctgctcggt atcccgttcg gctggctggc ggacaaaggc tctcgcaaga
     2521 tgctgattgg cgtcggttta gtcgtctgga gcgtgttttc cggcttgacc ggatttgccg
     2581 ccgatttctg gacgcttttt ttctgtcgcc tgatggtcgg cgtcggcgaa gcgactttgg
     2641 gtccggcggc gatttcgctt ttgagcgatt attttccgcc gacaaaacgc gcgaccgtga
     2701 cgagcatcta ttcgatggga atcgccgtcg gcgcgggtct ggcggcattt ttaggcggct
     2761 ggataggctt gaattacggc tggcgctggg ctttttatct gctcggtttt cccggaatcg
     2821 tcatcgcctt gctggttttt ttgttgcgcg aaccggcgcg cagccaggac gcgaaacaaa
     2881 cgggagcgga atactcgcgc gccgattgga aaaatctttt gaccggcaag ccttttattt
     2941 tgctttgtct cggctacgcg ttcctcgggc tggcgaccaa caatctttcg atttggggcg
     3001 cggtttttta cacgcgcgtt ttcgggctca acattgcgac catcggtttt tacacgggaa
     3061 ttttcgtaat tatcgcggga attccggcga cgctctacgg cggcgttctg gctgactggt
     3121 tccggcgcaa atcgcgcggc gggcggatgc tttacggcgc gattttgtgc gtgatttccg
     3181 ttcctttctg gctcgttctg ctgttcgcg
//
LOCUS       DASWNE010000196         3332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_606505, whole genome shotgun sequence.
ACCESSION   DASWNE010000196 DASWNE010000000
VERSION     DASWNE010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606505"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VGB68_04035"
     CDS             <1..550
                     /locus_tag="VGB68_04035"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEY0048430.1"
                     /translation="EIGDGCTIRSGVRITNSRIGHGVEILENSVIVDSEISNSCTVGP
                     MAHLRGHAKMEESAKVGNFVELKKTILGRGSKASHLTYLGDATIGEKTNIGAGTVTCN
                     YDGKNKHATTIGNNVKIGSDTMLVAPVKVGDGAVTAAGSVVTKDVPPDTLVAGVPAVV
                     KKNLKESEKDSISAQTDQPSEK"
     gene            586..726
                     /locus_tag="VGB68_04040"
     CDS             586..726
                     /locus_tag="VGB68_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048431.1"
                     /translation="MKKDKSKVKGSESTPEQVSPAEALKRMKSFSERKEKFVAAIRQS
                     QN"
     gene            698..1030
                     /locus_tag="VGB68_04045"
     CDS             698..1030
                     /locus_tag="VGB68_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048432.1"
                     /translation="MPLSARARIEVYLPERKTPAYRRLQKAFEREFLQTFGGCTVIEN
                     IKGLYLSSNEKIDSDVINLVYADTPFDFEKNFKALSKYTDELREAALEASEEESILIV
                     VHKIYHSV"
     gene            1042..2904
                     /gene="glmS"
                     /locus_tag="VGB68_04050"
     CDS             1042..2904
                     /gene="glmS"
                     /locus_tag="VGB68_04050"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEY0048433.1"
                     /translation="MCGIVGYVGNKQVVPLIIDGLRKLEYRGYDSAGIAVVDENNDLE
                     IRRAEGKLRNLEECIRLSPLDGNYGIGHTRWATHGRPTEENAHPHRDCTGRIVVVHNG
                     IIENYLSLKEKLKEQGHVFKTETDTEIVAHLIEEFMKSENLGFEQAVQKTVRHLRGIF
                     ALSIISADEPDMIIAVREGPPVVIGLGDGEFFVASDIPAILQHTRDVFYLGEKEIAVL
                     TKDSVRVTDFDGNIVEPKQQRITWDPIMAEKGGFKHFMLKEIYEQPRAVRDTVRGRIS
                     LDTGNIYLDAMNISEEEFRAVTSIKIAACGTSWHAGLAGKYMIEQLARLSVEVDYASE
                     FRYRQPVLDENTLLIVISQSGETADTIAAMREARSAGCKVMAVCNVQGSMIHREADGT
                     ILTHAGPEIGVASTKAFTSQMIALYLFALYLGQLRGKLDEAQSKYHAQQLAELPLKIE
                     HLLNDADSIEELSREFFRSQDFLYLGRGINFPVSLEGALKLKEISYIHAEGYPAGEMK
                     HGPNALIDEKLPVVIVNTREAGNAASEIRYEKTHSNIVEVKSRDGIVISILTEGDEMS
                     SHVSDHVIEIPETSDLLAPILSIVPLQLLAYHIAVRRGCDVDQPRNLAKSVTVE"
BASE COUNT          904 a          827 c          872 g          729 t
ORIGIN      
        1 cgaaatcggc gacggctgca cgattcgctc cggcgtgcgg attaccaatt cgcgcatcgg
       61 tcacggcgtc gaaattctgg aaaactcggt gattgtcgat tcggaaatat cgaacagctg
      121 cacggtcgga ccgatggcgc atttgcgcgg tcacgccaag atggaagaat ccgccaaagt
      181 cggaaatttt gtcgaattga agaaaacaat tctcggtcgc ggctcgaaag ccagtcatct
      241 gacttacctg ggcgacgcga ccatcggcga aaaaaccaac atcggcgcgg gcaccgttac
      301 ctgcaattac gacggcaaaa acaagcacgc gacgaccatc ggcaacaacg tgaaaatcgg
      361 ctcggatacg atgctcgtcg cgcccgtcaa ggtcggcgac ggcgcggtca cggcggcagg
      421 ctcggtcgtc acgaaagacg ttccgcccga tactctggtc gcgggcgttc cggctgtggt
      481 gaagaaaaat ttaaaggaat ccgagaaaga ttcgatttcg gctcaaaccg accagccgtc
      541 cgaaaaataa tctcaggcaa gcctcggaga atttaaagtt tttgtatgaa gaaagataaa
      601 tcaaaggtta aagggagcga atcgacgccc gaacaggtct cgcccgccga agccttgaaa
      661 agaatgaaaa gtttttcgga aagaaaggag aaattcgttg ccgctatccg ccagagccag
      721 aattgaagtt tacctgcccg aaagaaaaac gcccgcttac cggcgcttgc agaaagcgtt
      781 cgagcgggaa tttttgcaga ccttcggcgg ctgtacggtt attgaaaaca tcaaaggatt
      841 gtatctttct tcaaatgaga agattgattc ggatgtaatc aatctggttt acgccgacac
      901 gccgtttgat ttcgagaaaa atttcaaggc gctttcaaaa tacacggacg aattgcgcga
      961 agcggcgctc gaagccagcg aagaagaatc gattttaatc gtcgttcata aaatttatca
     1021 ttcggtttaa aataaaaaat tatgtgtgga attgttggat acgttggaaa taaacaggtc
     1081 gtgccgttaa tcatcgacgg tttgcgaaaa ctcgaatatc gcggatatga ttccgccgga
     1141 atcgccgtcg tcgacgaaaa taacgacctc gaaatccgcc gcgccgaagg gaaattgcgc
     1201 aatctcgaag aatgcatccg gctcagcccg ctggacggaa attacggcat cgggcacacg
     1261 cgctgggcga cgcacgggcg accgaccgag gaaaacgcgc acccgcaccg cgattgcacg
     1321 ggcagaatcg tcgtcgtcca caacggcatt atcgaaaatt atctgtctct gaaagaaaaa
     1381 cttaaagagc agggacacgt tttcaaaacc gaaaccgata cggaaatcgt cgcgcactta
     1441 atcgaagaat ttatgaaatc cgagaatctc ggtttcgagc aagccgtcca gaaaaccgtg
     1501 cgccatttgc gcggaatctt cgcgctttcg attatctcgg cggacgagcc cgatatgatt
     1561 atcgccgtcc gcgaaggtcc gccggtcgtc atcggcttgg gcgacggcga atttttcgtc
     1621 gcctcggaca ttcccgccat tttgcagcac acgcgcgacg ttttttatct cggcgaaaag
     1681 gaaatcgcgg ttttgacgaa agactcggtg cgcgtcaccg attttgacgg aaacatcgtc
     1741 gagccgaaac agcagcgcat cacgtgggac ccgattatgg cggaaaaggg cggcttcaag
     1801 cattttatgc tgaaggaaat ctacgagcag ccgcgcgccg tccgcgacac cgtgcgcggc
     1861 agaatctcgc tcgatacggg caatatttat ctggacgcga tgaatatctc cgaagaagaa
     1921 tttcgcgccg taacgagcat caaaatcgcc gcctgcggaa cgtcctggca cgccgggctc
     1981 gccggaaaat atatgatcga gcagctcgcg cggctttcgg tcgaggtcga ctacgcgtcg
     2041 gagtttcgtt accgccagcc ggttctggac gaaaacactt tattaatcgt catttcgcaa
     2101 tcgggcgaga ccgccgacac gattgccgcg atgcgcgaag cgcggtcggc gggctgcaaa
     2161 gttatggcgg tctgcaacgt gcagggctcg atgattcatc gcgaagccga cggcacgatt
     2221 ctgacgcacg ccggaccgga aatcggcgtc gcttcgacaa aagcctttac atcgcagatg
     2281 attgcgcttt atcttttcgc gctttatctc ggtcagctgc gcggaaagct ggacgaagcg
     2341 caatcgaaat atcacgctca gcaattggcg gagctgcctc tgaaaatcga gcatctttta
     2401 aacgacgccg attccatcga agaactttcc agagaatttt tccgctcgca ggattttctt
     2461 tatctcggtc gcggcataaa ttttccggtt tcgctggaag gcgctttgaa gctcaaggaa
     2521 atttcctata ttcacgccga aggctatccg gcgggcgaga tgaaacacgg acccaacgcc
     2581 ctgattgacg aaaagcttcc cgtcgttatc gtcaatacgc gcgaagcggg aaatgcggcg
     2641 agcgagattc gttatgaaaa aacccattcg aacatcgtcg aagttaaatc gcgcgacggc
     2701 atcgtgattt cgattttaac cgagggcgac gaaatgagct cacacgtctc cgaccacgtt
     2761 atcgagattc ccgagacgtc cgaccttctc gcgccgattc tgtccatcgt gcctttacag
     2821 cttctggcgt atcacatcgc ggtcagacgc ggctgcgacg tcgaccagcc gcgcaacctg
     2881 gcaaaatcgg tgacggtcga gtagcttccg gaaagaatat cgacaaactt gtgcgataat
     2941 aaagtcgtat aattttaata aagttgtata atttcttccg cttgaggata gctttcaagc
     3001 ggaagatttt ttatgcccgt aaaaaatacg aatagaccga agccgaattt aaaaagtact
     3061 taaaagaagg taggacaagg aaccgggaaa gattataagc catagttgac gacctataat
     3121 gttccgtcgc gcggaagacg cgccggcgat atgggacgga aaagcggaag gacttcgcgg
     3181 atggaagcat cagcttgccg aaagttccga cttaaagtat ttaaggaaaa acttgaaaat
     3241 ctacaattat ttttttaata ttgtttaggg aaagttaaac tctctttttt ttcaaatccg
     3301 gttattgcaa atctttccag gaaatacaga tt
//
LOCUS       DASWNE010000197         4565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_77066, whole genome shotgun sequence.
ACCESSION   DASWNE010000197 DASWNE010000000
VERSION     DASWNE010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77066"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VGB68_04055"
     CDS             complement(<1..761)
                     /locus_tag="VGB68_04055"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEY0048434.1"
                     /translation="MNKKLSSLVSIAIFVLILSATAQAEIFFAYLNGAQEVPPVATSA
                     TGYARVFLNESAGTISYTVVFNNLSSNQTASHIHTGAIGVSGPVTINFGAVGGTSGTI
                     TGTAPITPAQIALLRSHQFYVNVHSANFTPGEIRGQLGVKRPVDFDGDGRNDFSVLRF
                     PNVAPPGQAPITFYNLNSTTGFQSVPFGNANTDFPAPGDYDGDGRDDITLYRRGAAAG
                     QQSFFHLIRSSDGTLQSTQFGLFGDLTVARDYDGDG"
     gene            1114..1725
                     /locus_tag="VGB68_04060"
     CDS             1114..1725
                     /locus_tag="VGB68_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048435.1"
                     /translation="MFTLFGTVAVKAAAVSSAEECLAEMAQETADYMRSRGLQITNSS
                     YSFANSNGEKVYSLTSTDSVKAGARLNRTQSVIQHKNSEGQSDFYVINLDVQSEQMTY
                     TIYRNSALHDKVELALYVPGGGSDPCAGALTQSQVDTILQNLLAQANADCKTRYTCLF
                     QQMGPSCAYVMYMIKPTSWRCRIIHVDVQNAIDRAISRTSVTP"
     gene            complement(1895..2071)
                     /locus_tag="VGB68_04065"
     CDS             complement(1895..2071)
                     /locus_tag="VGB68_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048436.1"
                     /translation="MREKSGNAIEESGAAFALAIVLLNILTVVAVGGLGWLLQMIITL
                     DSPFGTALREAAWR"
     gene            2333..3409
                     /locus_tag="VGB68_04070"
     CDS             2333..3409
                     /locus_tag="VGB68_04070"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0048437.1"
                     /translation="MLDSGNSFNETKAEQFADKLVSMLNGAATALMISIGHRTGLFDA
                     MADLPSSNVGRIAAAAGQNERYVREWLGAMATGKIVDYDAQTQTYRLPKEHAAFLTRL
                     SAPNNLAAVAQYIPLLGAVEDKIVECFKNGGGVPYSAFPRFHEVMAEDSGQTVLPALI
                     DSILPLVEGIREKLEQGIEVLDIGCGSGRAINLLAKTYPNSFFYGYDFSGQAIAAARR
                     EADKLGLKNIRFEVRDAATLDEPNSFDLITAFDAIHDQAKPAAVLKAVNKALRPHATF
                     LMQDIAGSSHVHKNMEHPIAPLLYTVSCLHCMTVSLAENGDGLGAMWGEEKALEMLAA
                     AGFGKVAVHRLPHDFQNQYYVASK"
     gene            3768..>4565
                     /locus_tag="VGB68_04075"
     CDS             3768..>4565
                     /locus_tag="VGB68_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048438.1"
                     /translation="MEEDLPTPNEYRTASGAPGSRYWQQRADYVIDVELDDVNQRIIG
                     RETVTYKNLSPDALNYIWLQIDQNIFAKDSLQQKTSTAPEFRNMPLSRVEQLAAREYE
                     GRVNLTAVRDARGNPLKITLNETMLRIDLPTPLAPNANFVFSVEWNYNINSGALYGGR
                     TSYEYFPEDNNYLYTIAQWFPRLAAYNDSNGWQHKQYLGAGEFTLEFGDYLVRITAPD
                     DHVVSATGVLQNPNQVLKPVWRDRLKQAETAKEPIYIVTKEEAAANEK"
BASE COUNT         1125 a         1177 c         1176 g         1087 t
ORIGIN      
        1 ccgtcgccgt cgtaatcgcg cgcgaccgtt aaatcgccga aaagtccgaa ctgcgtcgat
       61 tggagcgttc cgtccgagct gcggattaaa tgaaagaagc tttgctgtcc ggctgccgcg
      121 ccgcgtcgat acaatgtaat atcgtcgcgc ccgtcgccgt cataatcgcc cggcgcggga
      181 aaatccgtat tcgcgttgcc gaacggcacg gactgaaaac cggtggtgct gttcagattg
      241 taaaaggtta tcggagcctg tccgggcggc gcgacgttcg ggaaacgcag gacgctgaaa
      301 tcgtttctgc cgtcgccgtc gaaatcgacg ggacgtttga cgccgagctg accgcgaatc
      361 tcgccgggcg tgaaattagc cgaatgcacg ttgacgtaaa attgatgcga gcgcagcagc
      421 gcgatttgcg ccggcgtgat tggcgccgtg ccggtaatcg tgccggaagt gccgccgacc
      481 gcgccgaaat taatggtgac cggtcccgat acgccgatgg cgccggtatg aatatgcgac
      541 gcggtctggt tgctggacag attgttgaat acgaccgtat acgaaatcgt tccggctgat
      601 tcgttcagaa atacgcgcgc gtatccggtg gcggaggtcg cgaccggcgg aacttcctgc
      661 gcgccgttta aatacgcgaa aaagatttcc gcctgagccg tcgccgacag aataagcaca
      721 aatatcgcaa tgctaaccaa gctacttaac tttttgttca ttttcccctc gaatgttaat
      781 ggatttaatt tgtctccgat ttaagtgaat gtagtttatt taccggcggg tgtcaatatt
      841 tttcggttaa aacaaaaaga ttagccagta tttaccggct atttcgagaa gaaaggattt
      901 ttgacgcggg ctggcgcggg cgggtgtttt attgacttta aaataattgt tctgataacc
      961 tgctgatgtt tttcccaaaa aaaataaaat aaaggaagaa gaatgtttcc cacaaaactt
     1021 ttctcgtcgt ttgcgggaag cgtccacaaa catatgaaaa aaacagttaa atgcctacga
     1081 aatatcgcag ctttactttg tacggctgct tttttgttta ctcttttcgg aacggtcgcc
     1141 gtcaaagccg ccgccgtcag cagcgcggaa gaatgtttag ccgaaatggc tcaggaaacc
     1201 gccgactata tgcgttcgag aggacttcaa ataaccaatt cttcttattc tttcgctaac
     1261 agtaacggag aaaaggttta ttcgctgact tcgacggatt ccgtcaaagc cggcgcgcgc
     1321 ttaaacagaa cccagtcggt catccagcac aaaaacagcg agggacaaag cgatttttac
     1381 gtcatcaatc tggacgtgca gtccgaacag atgacctaca cgatttacag aaattctgcg
     1441 ctgcacgata aggtggaatt ggctttatac gttccgggcg gaggttccga cccctgcgcc
     1501 ggagctttga cgcagtcgca ggtcgatact atattgcaga atcttctggc gcaggcaaac
     1561 gcagattgca agacgcgtta tacctgcctg tttcagcaaa tgggtccgtc ctgcgcatat
     1621 gttatgtata tgataaaacc gacttcgtgg agatgcagaa ttatccatgt cgacgttcag
     1681 aacgccatcg acagggcgat ttcaagaact tcggtcactc cgtaacccgg taatcgataa
     1741 ttcataaaaa gagcaatctc cgggttgctc tttttattta cgctttacct cgccgaaaag
     1801 tcggagttga gaattgataa ttgagcggca tttgtcgatt gccgctcaac ctttcaatag
     1861 ttttcgattc gacgctccgc aagcgccgcc gtctttatcg ccacgcggct tcgcgcagag
     1921 ccgtgccgaa cgggctgtcg agcgtgatta tcatttgcag cagccaacca agcccgccga
     1981 cggcgacgac ggtcaaaatg tttaacagca cgatggcgag cgcaaacgcc gcgccgcttt
     2041 cttcaatcgc atttcctgat ttttctcgca tctctgattt ctccttttta cgctttttta
     2101 aaactcgact tttagctttc ggggcttttt cggattcatt ccgcttcgat aaatcgcttc
     2161 tatcaattaa cgcgacgacc ttgaccgaat gcgctcattt ttctcagaaa aatttcagaa
     2221 aaatttcaga acgcgctaaa gattctttga cggcgattgg gaataatggc tagcgtccgg
     2281 cagtttccgt gaacgaccgg acggaattta tattttctgg aggtctttat atatgttgga
     2341 tagtggaaat tcttttaatg aaacgaaagc cgaacaattt gccgataaac tggtgtcgat
     2401 gctcaacggc gcggcgacgg cgttgatgat cagcatcggg caccgaaccg gcttgttcga
     2461 cgcgatggcg gatttgccgt cgtcaaacgt cgggcggatt gccgccgccg ccggtcaaaa
     2521 cgagcgatac gtgcgcgaat ggctcggcgc gatggcgacc ggaaaaatcg tggattacga
     2581 cgcgcagacg caaacttatc gcctgccgaa agaacacgcg gcgtttctga cgcgcttgtc
     2641 cgcgccgaat aatctggcgg cggtcgcgca atatattccg cttctcggtg cggtcgaaga
     2701 caaaatcgtc gagtgtttta aaaacggcgg cggcgttcca tactccgctt ttccgcgttt
     2761 tcacgaggta atggcggaag acagcgggca aaccgttctg cccgcgctga ttgattcgat
     2821 tctgccgctg gtcgaaggga ttagagaaaa gctggaacag ggaatcgagg tcctggatat
     2881 cggctgcggc agcgggcgcg cgataaacct gctggcgaaa acttacccga acagcttttt
     2941 ctacggctac gatttttccg ggcaggcaat cgccgccgcc cgccgcgaag ccgataaact
     3001 gggcttgaaa aacatccgtt tcgaagtgag agacgcggcg acgctggacg agccgaacag
     3061 cttcgatttg attacggctt tcgacgcgat tcacgaccag gcgaaaccgg cggcggtttt
     3121 aaaggcggtc aacaaagccc tgcgcccgca cgcgactttt ctgatgcagg acatcgccgg
     3181 ttcctcgcac gttcataaaa atatggagca cccgattgcg cctctgcttt acaccgtctc
     3241 gtgcctgcat tgtatgacgg tttccttagc cgaaaacggc gacgggctgg gcgcgatgtg
     3301 gggcgaggaa aaagcgctgg aaatgctcgc cgccgccggt ttcggcaaag tcgccgtaca
     3361 tcgcctgccg cacgactttc aaaatcagta ttacgtcgcg agcaagtgaa aaaacggtaa
     3421 gcggtaaacg gtaaacggta agttgcagct gccgattatt gagtttcaaa taagaactcg
     3481 atattcaaaa ctacgaactt accgcttacc gtttaccgct taccgtttta aaatgcttgc
     3541 atttcgagtc attatttccg aaactaaaaa taaacgtctg cgtaaaagtt accaagctat
     3601 taatttgtcc ggctttcccc cagcgtttta gtttattagt gaatctattt ttgaggagaa
     3661 aagcaagcgt gttattacgt aaagttttcg cttttattat tgtcttgtcg tgcgccggtt
     3721 ttgttttcgc acagtcgaaa tcaaagcctt ccgacaaatt taaacagttg gaagaagacc
     3781 tcccgacgcc gaacgaatac cggacggctt cgggcgcgcc cggcagtcgt tactggcagc
     3841 agcgcgccga ttacgtcatc gacgtcgaac tggacgacgt caaccagcgc attatcggca
     3901 gggaaaccgt cacctataaa aatctttcgc ccgacgcatt gaattatatc tggctgcaaa
     3961 tcgaccagaa tattttcgcc aaagattctt tgcagcagaa aacatcgacc gcgccggagt
     4021 ttcgcaatat gccgctttcg cgcgtcgaac aactcgccgc gcgcgaatac gaagggcgcg
     4081 tcaacctgac cgccgtgcgc gacgcgcgcg gaaatccgct caaaatcact ctgaacgaga
     4141 cgatgctgcg cattgatttg ccgacgccgc tcgcgccgaa cgccaacttc gttttctcgg
     4201 tcgaatggaa ttacaacatc aatagcggcg cgctctacgg cgggcgcact tcttacgaat
     4261 attttcccga agacaataat tatctataca ccatcgcgca gtggtttccg cgcctggcgg
     4321 cgtataacga ctcgaacggc tggcagcaca agcagtatct cggcgcgggc gaatttacat
     4381 tggaattcgg cgattattta gtcagaatca ccgcgcccga cgaccacgtc gtcagcgcga
     4441 ccggcgtttt gcagaatccg aatcaggttc tcaaacccgt ctggcgcgac cgtctgaaac
     4501 aagccgaaac cgctaaagag ccgatatata tcgtcaccaa ggaagaagcc gccgccaacg
     4561 aaaaa
//
LOCUS       DASWNE010000198         1629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_676290, whole genome shotgun sequence.
ACCESSION   DASWNE010000198 DASWNE010000000
VERSION     DASWNE010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676290"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..836)
                     /locus_tag="VGB68_04080"
     CDS             complement(162..836)
                     /locus_tag="VGB68_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048439.1"
                     /translation="MYLDAVNQTNETNIIETTNGLGVAKAPAEVFEKWQDWHPSQISH
                     HGQMCCEIAREWITATDFSELGGGSLYAGPRWLRQKFNWGASSFPIYWCEAVRRHTLD
                     CGALAALAHEVFTVRGVESYRVQLVQKFSQVATTQWSCSWSDGDATELRWIDKDLIYH
                     EGCAIAVGKGEIKVWDASAGWWVDPKTGDGYGSLVALRISANNARAPFVWGDRRIIAN
                     QWQKIN"
     gene            complement(817..>1629)
                     /locus_tag="VGB68_04085"
     CDS             complement(817..>1629)
                     /locus_tag="VGB68_04085"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048440.1"
                     /translation="REALSDASFFTVFCDSIEELNRVIAGIETGELKTQYLGIRLRTP
                     NIRSRFGITIDTPETFQNLVETVRRIPKQCRFGVHFHMASSNVGVQQWWHLFESMLRW
                     CSSIEALSGRLIEIMDVGGGWFPEDLQKNSGEKFRKAIEMIPEFLPNVKQIFSEPGKA
                     LAQPSMAVAMQILEIRNAFGDAREVVVDGSIAELPMYFFYPHRILCQDGKNGGWKSLN
                     RGKTQLLGRLCMEHDIVASNVELPENAQVGDILVFCDAGAYDRSMSYVFGRG"
BASE COUNT          388 a          460 c          385 g          396 t
ORIGIN      
        1 ctgtttttta ttttgatgaa acggcttcaa tttttattta agacgtttta gaaaaaagaa
       61 ccagagagta ttggaaccat gaacgataaa tgatgaattc gcaattcatc atttatcgtt
      121 tcccacattt ttgcgctaaa aaatcttatc gcaaattgac gttaatttat cttttgccac
      181 tggttcgcga taatgcggcg gtcgccccaa acaaacggcg cccgggcgtt attcgccgaa
      241 atgcgcaacg cgacgagcga gccgtaaccg tcgccggttt tcgggtcgac ccaccagccc
      301 gcgctggcgt cccagacttt gatttcgccc ttgccgacgg caatcgcgca gccttcgtga
      361 taaatcaaat ctttatcaat ccagcgcagt tccgtcgcgt cgccgtcgct ccagctgcaa
      421 gaccattgag tcgtggcgac ctgcgaaaat ttctgcacca attggacgcg atagctttcg
      481 acgccgcgca cggtaaaaac ttcgtgtgcc agcgcggcga gcgcgccgca gtcgagcgta
      541 tgacggcgaa cggcttcgca ccagtaaatc ggaaacgaag acgcgcccca gttaaatttt
      601 tgtctgagcc agcgcggtcc cgcgtagagg ctgccgccgc caagctcgga aaaatccgtc
      661 gccgttatcc attcgcgggc aatttcgcag cacatctgcc cgtgatgcga gatttgcgac
      721 ggatgccaat cctgccattt ttcaaaaacc tcggcgggag ccttcgctac gccgagcccg
      781 tttgtcgttt caataatatt cgtttcgttt gtttgattaa ccgcgtccaa atacataact
      841 catactccta tcgtaagctc cggcatcgca gaagacgaga atgtcgccga cctgcgcgtt
      901 ttcgggtaat tcaacgtttg aggcaacaat gtcgtgttcc atgcaaagtc tgcctaaaag
      961 ctgtgttttt cctctgttta atgatttcca gccgccgttt tttccatcct ggcacaaaat
     1021 ccggtgcggg tagaaaaaat acatcggaag ctcggcaatc gaaccgtcaa cgacgacctc
     1081 gcgggcgtcg ccgaaagcat ttctgatttc cagaatctgc atcgccaccg ccatcgaagg
     1141 ttgagccagc gccttgcccg gctcggaaaa aatctgtttc acgtttggca gaaattccgg
     1201 aatcatctca atcgctttac ggaatttctc gcccgagttt ttctgcaaat cttccggaaa
     1261 ccagccgccg ccgacgtcca taatttcaat cagccgaccc gagagcgctt caatcgagct
     1321 gcaccagcgc agcatcgatt caaataaatg ccaccactgc tgaacgccga cattgctgct
     1381 cgccatatga aaatgcacgc cgaaccggca ttgtttcgga attcgccgga cggtttcgac
     1441 cagattttga aaagtttccg gcgtgtcgat cgtaattccg aaacgcgagc gaatattcgg
     1501 cgttctgagc ctgatgccga gatactgcgt tttcagctcg cccgtctcga tgcccgcgat
     1561 aacgcggttc agttcttcaa tcgaatcgca aaagaccgtg aaaaacgacg cgtccgacaa
     1621 agcttcgcg
//
LOCUS       DASWNE010000199         2187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_253682, whole genome shotgun sequence.
ACCESSION   DASWNE010000199 DASWNE010000000
VERSION     DASWNE010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253682"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VGB68_04090"
     CDS             <1..185
                     /locus_tag="VGB68_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048441.1"
                     /translation="ENNSRENLLEALERRRTLAETRERAYTYAEQAKKNLEILPVSRY
                     RSALDEIPSYMIERAK"
     gene            270..920
                     /locus_tag="VGB68_04095"
     CDS             270..920
                     /locus_tag="VGB68_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048442.1"
                     /translation="MLDENLIATLEQSLRQTRNAQNRLLVRLREVESESERLRQEIEA
                     LENSAAQTEAAIYSLLATMRSGQHLWNTDTTKIQRNEEDYEPPTEMKRINRPLPQNNN
                     QNNTSNNYRNNSNTVSYINNNSRNVPSVSPHIEPISHRFTDRTITQACTLLLRESGKP
                     LHVNELYNLLCVGGMEFKGNNPTISIAVSLNRNRRFRKVAPGTFDLVMRDASQAAS"
     gene            1037..>2187
                     /locus_tag="VGB68_04100"
     CDS             1037..>2187
                     /locus_tag="VGB68_04100"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048443.1"
                     /translation="MSIRGIKKSKISFVFIGLFVLLAGLSANVAAQKVRLRAQITPNC
                     GSNSNLKFSDIYADGNIAVQGSYNCRGAFIYNVSNPDAPTLSAWYNPGANQQFLEAIV
                     VGNRGYFGSGNGGGVHIVDLTNPTAPVLLGVVNAANGNGFASIHEMVVFSQNGATYLI
                     ENFNGFSSKILKVINVSNPAAPVFVRDINPTEVSWVHAMHLRGNRMFTSGWGTNTTRG
                     RTEIYDITNINTQAPALLGFIEDPTSTTAGNSMHSSWTSEDGNFLYSCRETSNGNGDV
                     RVYNVSTPAQPLLVKRIGMNDLALNAVTPHNPVVMGNKLYVAWYQAGLQVFDIGTNPA
                     DPKRIGQYDTYQAAFAPTEEETTSLLNAEPWDMVCAGDNLQNALPTTYDG"
BASE COUNT          555 a          588 c          554 g          490 t
ORIGIN      
        1 acgaaaacaa ttcgcgcgaa aatcttctgg aagcgctgga acgccgccga acgcttgccg
       61 aaacccgcga acgagcatac acttacgctg aacaagccaa gaaaaacctt gaaattttgc
      121 ccgtttcgcg gtatcgttct gctttggacg aaattccgtc ttatatgatt gaaagagcga
      181 aatagccatc agccatcagc ggtcagccgt cagcctttag ctgattggtt agctgaatgc
      241 tggtagctga tagctgaatg ccgattgcta tgcttgacga aaatcttatc gccactctgg
      301 aacagagcct gcggcagacg cgcaacgcgc agaaccgtct tttagtgcgt ctgcgcgaag
      361 tcgaatccga atccgaacgg ctgcgtcagg aaatcgaagc gctggaaaac agcgcggcgc
      421 agacggaagc ggcgatttac agtctgctgg cgacgatgcg ctccggtcag catctgtgga
      481 ataccgacac caccaagatt cagcgcaatg aagaagatta cgagccgccg acggaaatga
      541 agcggattaa tcgccctttg ccgcaaaata acaatcaaaa taacacgtcg aacaattatc
      601 ggaacaactc caacacggtt tcttacatca ataataattc ccgcaacgtc ccttcggtca
      661 gcccgcatat cgagccgatt agccaccggt tcaccgaccg cacgattacg caggcatgca
      721 cgctgctgct gcgcgaatcc ggaaagcctc tgcacgtcaa cgagctgtat aatctgctct
      781 gcgtcggcgg aatggaattt aagggaaata atccgacgat ttcgattgcc gtttctttaa
      841 accgcaaccg gcgttttcgg aaagtcgcgc ccggcacctt cgatctggtg atgcgcgacg
      901 cttcgcaggc ggcttcgtaa actgtattaa aatttatatt tagaggacta tttcaggttg
      961 cgttttttcg taaaacgcac caaaatagaa gtttagacag agccttcatt ttcgggcaga
     1021 ttcataggag ataaaaatga gtattcgggg cattaagaaa tcaaaaattt ctttcgtctt
     1081 tattggtttg tttgtcttgc tggcgggctt gagcgccaat gtggcggcgc agaaagtgcg
     1141 cctcagagcg cagattacgc cgaactgcgg ttcaaattcc aatttaaaat tttctgatat
     1201 ttacgccgac ggcaatatcg ccgttcaggg cagctacaat tgtcgcggag cttttattta
     1261 caacgtttcc aacccggatg ctccgactct ctccgcctgg tataacccgg gagcgaacca
     1321 gcagtttctc gaagccatcg tcgtcggcaa tcgcggctat ttcggcagcg gcaacggcgg
     1381 cggcgtgcac atcgtcgatt tgaccaaccc gaccgctccg gttctgctgg gcgtcgtcaa
     1441 cgcggcgaac ggcaacggtt tcgcttcgat tcacgaaatg gtcgttttca gccagaacgg
     1501 agcgacttat ctgattgaaa atttcaacgg tttttccagc aagattctga aagtcatcaa
     1561 cgtcagcaat cctgccgcgc ccgtttttgt ccgcgacatc aatccgaccg aagtcagctg
     1621 ggttcacgcg atgcacctgc gcggcaaccg aatgtttacg tccggctggg gcactaatac
     1681 cacgcgcggc agaaccgaga tttacgacat taccaacatc aatacgcaag cgccggcgct
     1741 tttaggcttt atcgaagacc cgacctcgac caccgccgga aacagtatgc acagcagctg
     1801 gacgagcgag gacggaaact ttttgtatag ctgccgcgaa acctccaacg gcaacggcga
     1861 cgtgcgcgtt tacaacgttt ctactccggc tcagccgctt ttagtaaagc gaatcggaat
     1921 gaacgatttg gcgcttaacg ccgtcacgcc gcacaatccg gtcgtgatgg gcaataaatt
     1981 gtatgtcgcc tggtatcagg cgggcttgca ggttttcgac atcggcacta atccggcaga
     2041 cccgaaacgc atcggtcaat acgacacgta tcaagcggct ttcgcgccga ccgaagaaga
     2101 aacgacgagt cttttaaacg ccgagccgtg ggatatggtc tgcgccggcg acaatctgca
     2161 aaacgctctg ccgacgactt atgacgg
//
LOCUS       DASWNE010000200         2356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_571312, whole genome shotgun sequence.
ACCESSION   DASWNE010000200 DASWNE010000000
VERSION     DASWNE010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571312"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2323)
                     /gene="lon"
                     /locus_tag="VGB68_04105"
     CDS             complement(<1..2323)
                     /gene="lon"
                     /locus_tag="VGB68_04105"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:NF008053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEY0048444.1"
                     /translation="MVPIRDVVIFPFTKVAFKIGRPSSVRALEQAMTGERNIFLATQH
                     DATVDEPSADQIYTVGTLGRILQAQRQDNGQIKVVVEGRERGQSVRVEQDEDGMFFAH
                     VRKVQSIDESGYRTDGLLQKIHGFVEQLLRVSPDAYTDALHASLRGISAAQIADAMSS
                     HLRIPVEEKQQLLEIFSVQERLQKLIEILETEIEKKQLDRSVHSRVKRQMDKTQREYY
                     LNEQIKAIHKELGRKDEKAEFDELKKRIEEAGMSEEAREKAMQEMARLEAMPPMSAES
                     TVSRTYIDWLINVPWQNRSEEINDLNEAERVLNEDHYGLEKIKDRILEYLAVRQLVKN
                     PKGTILCFVGAPGVGKTSLGKSIANATGRKFVRLSLGGVHDEAEIRGHRRTYIGSMPG
                     QIIQLLKRAGTVNPVILLDEVDKLGRDFRGDPSAALLEVLDPEQNNTFRDHYLDVDYD
                     LSQVFFIATANVLHTIPAPLQDRLEILRLSGYTEREKVEIAKRHLMPKQAETNGLDAE
                     KVKFTDDGVLEVIRHYTREAGVRNLEREISSCLRKVAKNFVVHENKDDFRLVIDAEKV
                     RELLGTIRFRKQDVARVSEVGLVNGLAWTEVGGDVLQVEATLVRGKGEVTLTGQLGDV
                     MKESARAALTCVRTRAEKLGISHDDFREKDLHIHVPEGAIPKDGPSAGITLATAMVSA
                     LTGKAARHDVAMTGEITLRGKVLPIGGVKEKLLAAHRFGLKTIIMSKDNEKDLADVPE
                     EVREDLTIHLVEMIDEVLKLALVEEKSEEILEVE"
BASE COUNT          461 a          673 c          603 g          619 t
ORIGIN      
        1 gttcgacttc cagaatctct tcggattttt cttccaccag agccagcttc aacacttcgt
       61 caatcatttc gaccaggtga atcgtcaaat cctcgcgaac ttcttccgga acgtcagcca
      121 aatccttttc gttgtctttc gacataataa tcgttttcaa gccgaaacga tgcgcggcga
      181 gcagtttttc cttgacgccg ccgatgggca ggactttgcc gcgcagtgtg atttcgccgg
      241 tcatcgccac gtcgtgacgc gcggctttgc cggtcagcgc ggaaaccatc gccgtcgcca
      301 gcgtaatacc ggcgctcggt ccgtctttcg gaatcgcgcc ttcgggaacg tggatgtgca
      361 aatctttttc gcggaaatcg tcgtgactga taccgagttt ttcggctcgc gtgcggacgc
      421 aggtcaacgc cgcgcgcgcc gattccttca tcacgtcgcc gagctgcccg gtcagagtga
      481 cttcgccttt gccccggacg agcgtggctt cgacctgcaa aacgtcgccg ccgacttccg
      541 tccaagccaa gccgttgacc agcccgactt cgctgacgcg ggcgacgtcc tgtttgcgga
      601 agcgaatcgt tcccaaaagc tcgcgaactt tttccgcgtc aatcaccaga cgaaaatcgt
      661 ctttgttttc gtgaacgaca aaatttttgg cgaccttgcg cagacacgag ctgatttcgc
      721 gttccagatt tctgacgccc gcctcgcgcg tgtaatgacg gatgacttcc agaacgccgt
      781 cgtccgtaaa cttcactttt tccgcgtcga gcccgttggt ttccgcctgt ttcggcatca
      841 aatgacgctt ggcgatttcg accttttcgc gctcggtgta gcccgaaagg cgcaggattt
      901 ccaggcggtc ttgaagcggc gcgggaatcg tgtgcagaac gtttgccgtc gcgatgaaaa
      961 agacctgcga caaatcgtaa tcgacgtcca gataatggtc gcggaaagta ttgttttgtt
     1021 ccgggtctaa aacttccagc agcgccgccg acgggtcgcc gcgaaaatcg cgtccgagct
     1081 tatcgacttc gtccagcaga ataaccggat tgaccgttcc ggcgcgtttc aaaagctgta
     1141 taatctgtcc gggcatcgag ccgatatagg ttctccggtg accgcgaatc tcggcttcgt
     1201 cgtgaacgcc gcccaagctg agccgcacga attttctgcc cgtcgcgttg gcgattgatt
     1261 ttccgagaga ggttttgccg acgccgggcg cgccgacgaa gcaaagaatc gtgcctttcg
     1321 gatttttcac cagctggcgc acggctagat attccagaat tctgtccttg attttttcca
     1381 agccgtaatg gtcttcgttt aaaactcttt cggcttcgtt caaatcgttg atttcttccg
     1441 agcgattttg ccacggaacg tttatcagcc aatcaatgta tgtgcgggaa accgtgcttt
     1501 cggcggacat cggcggcatc gcttcgagcc gcgccatttc ctgcatcgct ttttcgcgcg
     1561 cttcctcgct cattccggct tcttcgatac gtttctttaa ctcgtcaaat tcggcttttt
     1621 cgtccttgcg cccgagttct ttgtgaatcg ctttgatttg ctcgttcaga taatattcgc
     1681 gctgcgtttt gtccatctgc cgcttgacgc ggctgtgaac cgagcggtcg agctgttttt
     1741 tctcgatttc cgtttcgaga atctcgatta atttttgcag gcgttcctgc acggaaaaaa
     1801 tttccagcag ctgttgtttt tcttcgaccg gaattctcag gtgcgagctc atcgcgtcgg
     1861 caatctgcgc ggcggaaatt cctcttaaag aagcgtgcag cgcgtcggtg taggcatcgg
     1921 gcgaaactct caaaagctgt tcgacgaagc cgtgaatttt ctgcaaaagc ccgtcggtgc
     1981 gataacctga ttcgtcaatt gactgaactt tccgaacatg cgcgaaaaac attccgtctt
     2041 cgtcctgttc gactctgacc gactgaccgc gctcgcgtcc ttcgacgacg actttgattt
     2101 gtccgttatc ctgccgctgc gcctgcaaaa ttctgcccaa agttccaacc gtataaatct
     2161 gatcggcgct cggctcatca acggtcgcgt cgtgctgcgt cgccagaaat atattacgct
     2221 cgccggtcat cgcctgttcg agagcgcgaa cggaactcgg acgaccgatt ttaaaggcga
     2281 ctttcgtgaa cggaaaaatg acgacgtcgc gaatgggaac catcggataa cgcttaatgt
     2341 ccgaaggcat ttggtc
//
LOCUS       DASWNE010000201         6485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_500057, whole genome shotgun sequence.
ACCESSION   DASWNE010000201 DASWNE010000000
VERSION     DASWNE010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6485
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500057"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1433
                     /locus_tag="VGB68_04110"
     CDS             <1..1433
                     /locus_tag="VGB68_04110"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY0048445.1"
                     /translation="HTEEYPELRIELQPQRFYPLGTTLAHVLGYVGEISPKQLEKPEY
                     AHFKPGDIIGKAGLEQYYDQHLRGVPGYRKVLVDSRGRVQSELEVVQPQAGQDLISTI
                     DIDLQLAAEDSLANSITKRGAIIAMDPRNGEILAMASLPSYDPNVFVKGSTTPEGRKQ
                     IAQYYTNEERPLLNRAIQGRYHPGSTWKIPESIAALQQGVITVKNSNVACGGGIQIGN
                     KHTRCMGSHGSPPVDYAITKSCDGYYYRLALKMGVDGLIKMVEEFEYDKPTGIDLPNE
                     KVTRTPKYWKPYVEKREGKWSDIRTVFASIGQDTVEATPISMLRAVSVVGVEGKMFVP
                     HLLKEFKEIGAVGEFPSRPAFGFAHPEPKIIEMTPEENNIILKGMWGVVNGGGTAAAL
                     KTEGFEIAGKTGTAQNSALNSGGAKDHAWFVSFAPAYKPEIAVLGLIENSGFGGTHAG
                     PAVKNVYNAFLQKNPSMIGGQQLAMR"
     gene            1511..2635
                     /locus_tag="VGB68_04115"
     CDS             1511..2635
                     /locus_tag="VGB68_04115"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEY0048446.1"
                     /translation="MVAIIEKRSLRDFDWLLAILAIAIVCFGIWQIHNAVPTENYWQK
                     QIIGLGIATVVMLVVSFTDYRRLVDLAPMFYVGGLILLILVLTPLGVKVNGQQAWLKL
                     PLIGQFQPSEFVKIPVVLMLAKYFGEKRRVGGVLRIKEFLIGVGILALPVSLIMLEPD
                     AGQSLTYFPLLAVVLFLSSIKIRYIVLAMLAGLMFVPLAFYIGVQTGKIKQYQIERVN
                     VILDPENADPRGYGYHTVQSMITVGQGGLTGIKGDSEKSQSALKFLPEPHTDFIFAVT
                     AENTGFLGCISLLLAYALLISRLIAGARHSPDRAGMLVIMSIVGGITFQIFINMGMAL
                     GFLPVIGVPLPLMSAGLSAVLSTFTAIGFVLSVRMRRFVN"
     gene            2799..5405
                     /locus_tag="VGB68_04120"
     CDS             2799..5405
                     /locus_tag="VGB68_04120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEY0048447.1"
                     /translation="MAKELDKLTREVETVPNGRRRNFWRYIWFPTALMLALAAGGLTG
                     VLGSYYLNNSSVTTEVSALATYRPPEVTKIYADDGETILAEFALEKRIPIKYADVPKT
                     VENALLAIEDYRFYQHNGIDPYRIAGAAVRNLTSNKTEGASTITQQLAKNLFLSREQT
                     YTRKITEWMMALQIERFYTKQQILEMYMNYIFLGAGSYGFEAGSRTYFGKPLKDVNLE
                     EAALLAAIPKSPEYSPTRNMEKAKERRDIVLDQMAKYQYISQAEAETAKAKPIKLADS
                     AYYQALPKSTAWDYPIEEIRKYLEEKYTTRIAQGGLQVYTTINVEAQKLATKSVREGL
                     RRYDRGRGWRSDYQNILLDENNENAPITDPKQIQRRLDSFKHADWYEDTYEKGEFIKG
                     LVVGIDAAKDEATVRFGKYKAILTAKDMGRSGKKPKDELKAGYLAEFEIKEVDDENQT
                     LKVELTQVPEVQASIVSINAKTGEVVAMVGGYDYATSKFNNATQGLRQTGSAFKPFIY
                     TAAVEWGMTADGVVSGAPIKRGGWMPHNYDGSLSHPNVPMKIALAKSYNLAAVHLLDQ
                     VGVQTGAQMVRRFGITNPMAPYLPSALGASEVSLFEMVSAYSAFPNKGVRMEPHLIRK
                     VLDRDGKVLEEWEKTSSKVTSEYVALTMVEMMQGVVKGGGTATNASASGHPLAGKTGT
                     VNDQTDVWFIGYTPTYVTGVWMGNPTKKESLGSGMTGGKGAVPFFNEFMIPFMKDKER
                     ETFPKAPPIPSDIKALVEQRKREELEKLEKAEEAGRKLGVSFNPGPRYNKTGDAPSSN
                     TTPTEPGLVVTPDDTTKPADNPPPNNPRSDDPKPNRPAATENPVKKPDPPVEKPKPEG
                     EKKKGKKGDDDN"
     gene            5571..>6485
                     /locus_tag="VGB68_04125"
     CDS             5571..>6485
                     /locus_tag="VGB68_04125"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEY0048448.1"
                     /translation="MLKNFTQPRRVAITGFGCVTPIGIGRAAFWQSLKTGKSGVSRIE
                     SFDVSDAKVKIAAEIKDFDWEAELNAKDRRHVPRTVPLALASAREALKDAQIQPENLS
                     LEEKRNFGVVLGTGGGGLSFTERQYVYWLTGEPQKASVYTIPASTHGGLSSELSMRFG
                     LHGLSHIVSTGCTSSTDAIAYAAQHIALGKQDMMLTGGVDTPLALGILKGFDLMTVLT
                     DKWNDAPEKASRPFDKKRSGIVLGEGAWIFVLENLENALLRNARIYAEISGYASTCDA
                     YHRVRLEENGVEPARAMTLALRDAGFAPE"
BASE COUNT         1664 a         1622 c         1806 g         1393 t
ORIGIN      
        1 cgcacaccga agaatatccc gaactccgca ttgagcttca gccgcagaga ttttatccgc
       61 ttggcacaac gctcgcgcac gttctcggct acgtcggcga aatcagcccg aaacaactgg
      121 aaaaacccga atacgcgcac tttaagccgg gcgacatcat cggcaaagcc ggactcgaac
      181 aatattacga ccagcatttg cgcggcgtgc ccggctaccg gaaagttctg gtcgattcgc
      241 gcgggcgagt ccagagcgag ctggaagtcg tccagccgca agccggacag gatttgattt
      301 cgacgattga catcgatttg cagctggcgg cggaagacag cctcgccaat tcgattacga
      361 agcgcggcgc gattatcgcg atggacccgc gcaacggcga gattctggcg atggcatcac
      421 ttccgagcta cgacccgaac gttttcgtca aaggcagcac gacgcccgaa ggacgcaagc
      481 aaatcgcgca gtattacacc aatgaagaac gtccgctttt aaatcgcgcc attcagggtc
      541 gttatcatcc gggctcgact tggaaaatcc ccgaatccat cgccgcgctt caacaaggcg
      601 tgataacggt caaaaactca aacgtcgctt gcggcggcgg cattcaaatc ggcaataaac
      661 acacgcgctg tatgggcagc cacggcagcc cgccggtcga ttacgccatt accaaatcct
      721 gcgacggtta ttattatcgt ctggcgctga aaatgggcgt tgacggttta atcaagatgg
      781 tcgaagaatt tgaatacgat aaaccgaccg gaatcgattt gccgaacgag aaagtcacgc
      841 ggacaccgaa atactggaag ccgtatgtcg aaaaacgcga gggaaaatgg agcgacatcc
      901 gcacggtttt cgcctcgatc ggtcaggaca cggtcgaagc gacgccgatt tcgatgcttc
      961 gtgccgtttc cgtcgtcggt gtcgaaggaa aaatgttcgt tccgcacctt ttaaaagaat
     1021 ttaaggaaat cggagcggtc ggcgagtttc cgtcgcgtcc cgccttcggc ttcgcgcatc
     1081 ccgagccgaa aattatcgag atgacgcccg aagaaaacaa tatcattctg aaaggaatgt
     1141 ggggcgtggt caacggcggc ggaacggcgg cggctttaaa aaccgaaggc ttcgagattg
     1201 ccggtaaaac cggaacggcg caaaactcgg cgctcaattc gggcggcgcg aaagaccacg
     1261 cctggtttgt cagtttcgct ccggcttaca aaccggaaat cgccgtgctc ggcttaatcg
     1321 aaaactccgg tttcggcgga acgcacgccg gtccggcggt caaaaacgtc tataacgcat
     1381 ttttgcaaaa gaatccgtcg atgatcggcg gtcaacaatt ggcgatgagg taatacattt
     1441 atcatcggaa atttaccatt tatcagttcg atttttggta agtgttcgat gataaagata
     1501 agtggtaaaa atggttgcaa ttatcgagaa acgcagtttg agagattttg attggctatt
     1561 ggcgattctg gcgatagcca ttgtctgttt cggcatctgg cagattcaca acgccgtccc
     1621 gaccgaaaat tactggcaga aacaaattat cggtttggga atcgcaaccg tcgttatgct
     1681 ggttgtttcc tttacggatt atcgtcgtct ggtcgatctc gcgccgatgt tttacgtcgg
     1741 cggcttgatt ttgctgattc tcgtgctgac gcctctgggc gtcaaggtca acgggcagca
     1801 ggcgtggctg aaactgccgc ttatcgggca attccaaccg tcggaattcg tcaaaattcc
     1861 ggtcgttttg atgctggcaa aatatttcgg cgaaaaacgc cgggtcggcg gcgttttaag
     1921 aataaaggaa tttcttatcg gcgtcggaat tctggctctg cccgtcagcc tgattatgct
     1981 cgaaccggac gcgggacaat ctctgactta ttttccgctt ctggcggtcg tgctgttttt
     2041 gtcgtcaatc aagattcgct acatcgtttt ggcgatgtta gccggtttga tgttcgtgcc
     2101 gctggcgttt tatatcggcg tccagaccgg caaaataaaa caatatcaga tagagcgcgt
     2161 caacgttatt ttagacccgg aaaacgccga cccgcgcggg tacggttatc acacggtgca
     2221 gtcgatgata acggtcgggc agggcggttt gaccggaatt aaaggcgatt cggaaaaatc
     2281 gcagagcgct ctgaaatttt tgcccgagcc gcatacggat tttatatttg cggtgacggc
     2341 ggaaaacacc ggatttttag gctgcatttc gctgctgctg gcttatgctc tgctgatttc
     2401 gagattgatt gcgggcgcgc ggcattcgcc cgaccgcgcc ggaatgctcg tgattatgtc
     2461 gattgtcggc ggaatcacgt ttcagatttt tatcaatatg ggaatggcgc tcggattttt
     2521 gccggtcatc ggcgttccgc tgccattgat gagcgccggt ctttcggcgg tgctctcgac
     2581 gtttacggca atcggcttcg tgctgagcgt gcgaatgcgg cgattcgtca attaacgatt
     2641 ttgattgcta agaaagactc gacagggatg aaagagatga acagggataa aacaaaagat
     2701 ttaaaaattt gtatttatct tcttcatctc cttcatccct gttaattttc tttctgtttc
     2761 ctgtgataaa atcgttttca tataaagagg ttttaaaaat ggcgaaagag ctagataaat
     2821 taaccagaga agtagaaaca gttccgaacg gcagaagaag aaatttctgg cgttacattt
     2881 ggtttccgac cgctttgatg ctggcgctgg cggcgggcgg tctgaccggc gtgctcggtt
     2941 cttattatct gaataattcg agcgttacga cggaagtttc cgctctggcg acgtatcgcc
     3001 cgccggaagt gacaaagatt tacgccgacg acggcgaaac gattctggcg gaatttgcgc
     3061 tggaaaaacg catcccgatt aaatatgcgg atgtgccgaa aacagtcgaa aacgcccttt
     3121 tggcaatcga ggattatcgc ttttaccagc ataatgggat tgacccgtat cgaatcgcag
     3181 gcgcggcggt cagaaatctg accagcaata aaaccgaagg cgcttcgacc atcacgcagc
     3241 agctcgccaa aaaccttttt ctctcgcgcg agcagaccta cacgcgcaaa atcaccgaat
     3301 ggatgatggc tctgcagatc gagcgttttt ataccaaaca gcaaattctt gagatgtata
     3361 tgaactacat ctttctcggc gcgggctcgt acgggtttga agccggttcg agaacgtatt
     3421 tcggaaaacc gctcaaggac gtgaatctgg aagaagccgc gcttctggca gcaattccga
     3481 aatcgcccga atattcgccg acgcgcaata tggaaaaggc gaaagagcgc cgcgacatcg
     3541 tcttagacca gatggcgaaa taccagtata tctcgcaggc ggaagccgag acggcgaaag
     3601 ccaaaccgat taagctcgcc gattcggctt attaccaggc gctaccgaaa tcgaccgcct
     3661 gggattatcc gattgaagaa atccgcaaat atctggaaga aaaatacacg acgcgcatcg
     3721 cgcagggcgg tttgcaggtt tacacgacga ttaacgtcga agcgcagaaa ctggcgacga
     3781 aatccgtccg cgaaggcttg cgccgttacg acagaggtcg cggctggcgc tccgattacc
     3841 aaaacattct gctcgacgaa aataacgaga acgcgccgat taccgacccg aaacagattc
     3901 agcgcaggct ggattcgttc aagcacgccg actggtacga agatacatac gaaaagggcg
     3961 agtttatcaa aggcttggtg gtcggcatcg acgcggcaaa agacgaagca accgtccgct
     4021 tcggcaaata taaagcaatt ttaactgcca aagatatggg cagaagcggc aaaaagccga
     4081 aggacgagct aaaggcgggc tatctggcgg agtttgaaat caaggaagtc gacgacgaaa
     4141 atcagacttt aaaggtcgag ctgacgcaag tgccggaagt tcaggcgtca atcgtttcga
     4201 taaacgccaa gacgggcgaa gtcgtcgcga tggtcggcgg ctacgattac gcgaccagca
     4261 aattcaacaa cgcgacgcag ggcttgcggc aaaccggctc ggcatttaaa ccttttattt
     4321 acacggctgc ggtcgaatgg ggaatgacgg cggacggggt ggtcagcggc gcgccgataa
     4381 aacgcggcgg ctggatgccg cacaattatg acggaagcct gagccacccg aacgtgccga
     4441 tgaaaatcgc gctggcaaaa tcctataatc tcgcggcggt tcacctgctc gaccaggtcg
     4501 gcgtgcagac gggcgcgcag atggttcgac gcttcggcat caccaatccg atggctccgt
     4561 atctgccgtc ggcgctcggc gcatccgaag tttcgttgtt tgagatggtt tcggcttatt
     4621 cggcttttcc gaataaaggc gtccggatgg agccgcacct gattcgaaag gtcttggacc
     4681 gcgacggcaa agtgctggaa gaatgggaaa aaacttcgtc gaaagtgacg agcgaatacg
     4741 tcgcgctgac gatggtcgag atgatgcagg gcgtcgtcaa aggcggcgga acggcgacca
     4801 acgcgagcgc gagcgggcat ccgctggcag gaaaaaccgg cacggtcaac gaccagaccg
     4861 acgtctggtt tatcggttac acgccgactt atgtgacggg cgtctggatg ggaaatccaa
     4921 ccaaaaaaga atcgctggga agcggaatga cgggcggaaa aggcgcagtt ccatttttca
     4981 atgagtttat gattccgttt atgaaggaca aggagcgcga aacttttccg aaagcgccgc
     5041 cgattccgtc cgatatcaag gctctggtcg aacagcgtaa gcgcgaggaa ctggaaaaac
     5101 ttgaaaaagc cgaagaagcc ggacgaaagc tcggcgtaag cttcaatccg ggaccgagat
     5161 acaacaagac cggcgacgcg ccttcgagca atacgactcc gacggaaccg gggctggtcg
     5221 tcacgccgga cgatacgacg aaaccggctg ataatccgcc gccgaacaat ccgcgcagcg
     5281 acgacccgaa accgaatcgt ccggcagcga cggaaaaccc ggtgaagaaa cccgacccgc
     5341 cggtcgaaaa accgaagcca gaaggcgaaa agaaaaaagg gaaaaagggc gacgacgaca
     5401 attaacagtc tacgagtttt gaattttgag cctggtcgat tttatttaaa actcgaaatt
     5461 caaaactcaa aatttaaaac tcaaagcgga acggttttca aactgttccg tcttttttgt
     5521 tttgccgttg tcgaaaatgt tatgctcagt tgtaagcagt tcaatatttt atgctcaaaa
     5581 atttcactca gccgcggcgt gtcgcaatta cgggtttcgg atgcgtgacg cccatcggaa
     5641 tcggacgcgc ggcgttctgg caatctctaa aaacaggcaa aagcggcgtc agtcgaatcg
     5701 agagttttga cgtttccgac gcaaaggtca aaatcgccgc cgagataaaa gattttgatt
     5761 gggaagcgga attaaacgcc aaagaccggc gacacgttcc gcgcacggtt ccgctggctc
     5821 tggcttcggc gcgcgaagct ttaaaagacg cgcaaatcca gccggaaaat ttatcgctcg
     5881 aagaaaaaag aaatttcggc gtcgtgctgg gaaccggcgg cggcggtctt tcattcaccg
     5941 aacggcagta tgtttactgg ctgacgggcg agccgcaaaa agccagcgtc tacacgattc
     6001 cggcttcgac gcacggcggt ttatcgagcg agctttcgat gcggttcggc ttgcacgggc
     6061 tttcgcacat cgtttcgacc ggctgcacca gctcgaccga cgcgattgcc tacgcggcgc
     6121 aacacatcgc gctcggcaaa caggatatga tgcttaccgg cggagttgac acgccgctcg
     6181 cgctcggcat tttaaagggc tttgatttga tgaccgttct gaccgacaaa tggaacgacg
     6241 cgccggaaaa agcctcgcgc ccgtttgata aaaaacgttc ggggatagtt ctcggcgaag
     6301 gcgcgtggat tttcgtgctg gaaaatctgg aaaacgcgct cttgagaaat gccagaattt
     6361 acgcggaaat ttccggttac gcttcgacgt gcgacgctta tcatcgcgtc cgcctggaag
     6421 aaaacggagt cgaaccggcg cgcgcgatga cgctggcgct gcgggacgcg ggctttgcgc
     6481 ccgaa
//
LOCUS       DASWNE010000202         2111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_253835, whole genome shotgun sequence.
ACCESSION   DASWNE010000202 DASWNE010000000
VERSION     DASWNE010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253835"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /locus_tag="VGB68_04130"
     CDS             complement(<1..1048)
                     /locus_tag="VGB68_04130"
                     /inference="COORDINATES: protein motif:HMM:NF014336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HEY0048449.1"
                     /translation="MLKNFATVLFLICLILPFSAFGWDDVGHKTTAYIAWRQLTPQAR
                     ERVSKILQNAPEDSNLGAFFMGDARSLGIRQMEHFMIASTWADIVRDRDFKVRYGKYH
                     KGNWHYSDTFWRGESGQATILPNPSEEGGRAVEKLIEFDRVLRSDSAPDAEKAIALAW
                     ILHLIGDLHQPLHTSARVTDLEPKGDQGGNLFLLTPKDTPRNRQENLHWFWDSIIVRN
                     TPRGDSCDTDFIPKIAASITKKHPATKMSSRLNLGKYDDWQQEGVRLAQTQVFSSALV
                     RYQTPTANYKKNAFQISQQQIALAGYRMGALLNQIFGNQTASQPDKDNYRKDAEASGS
                     KIGQGADDSRLWFKT"
     gene            1285..1554
                     /locus_tag="VGB68_04135"
     CDS             1285..1554
                     /locus_tag="VGB68_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048450.1"
                     /translation="MQAILEQNYIADALINRTSDAFGSKIGIVAKIFGCWHKELSRPF
                     ADKNKSYRACLHCGARKRFDAETLKTFGPFYYPPAISPVKSGRLN"
     gene            1701..2045
                     /locus_tag="VGB68_04140"
     CDS             1701..2045
                     /locus_tag="VGB68_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048451.1"
                     /translation="MGWLLFIICIAPLYLLYNTGQPIIWVITLVNAIANLWSYGVMHN
                     YAVQSSAKKIKQLQENLALEGRLNNQKQSEIDRQKLDIILQAVPNWLANVNILTFLIG
                     IGFLGYGIWLLI"
BASE COUNT          550 a          526 c          485 g          550 t
ORIGIN      
        1 gggttttaaa ccagagccgc gaatcatcag cgccctgacc gattttgctg ccgctcgctt
       61 cggcatcttt ccggtaattg tctttatctg gctgcgatgc cgtctggttt ccgaaaatct
      121 ggttgagcag cgcgcccatc cggtagcccg ccagcgctat ctgctgctgc gaaatctgaa
      181 aagcgttttt cttataattt gccgtcggcg tttgatagcg aaccagcgcg gacgaaaaga
      241 cctgcgtctg cgccagacgc acgccttcct gctgccagtc gtcgtatttg ccgagattca
      301 aacggctcga cattttcgtc gcaggatgtt ttttcgtaat cgacgcggcg attttcggaa
      361 taaaatccgt atcgcacgaa tcgccgcgcg gcgtgttgcg gacgattatc gaatcccaga
      421 accagtgcag attttcctgc cggttgcgcg gcgtgtcttt cggcgtcaga agaaataaat
      481 ttccgccctg gtcgcccttc ggctcaaggt cggtgacgcg cgccgacgta tgcagaggct
      541 gatgaagatc gccgattaaa tgcagaatcc acgccagcgc aatcgctttt tcggcgtccg
      601 gcgcggaatc gctgcgcaaa accctgtcga attcgatcaa tttttcgacc gccctgccgc
      661 cttcctcgct cggattcggc aaaatcgtcg cctgcccgct ttcgccgcgc cagaaagtgt
      721 cggaataatg ccagtttccc ttgtgatact tgccgtagcg aactttaaaa tcgcggtcgc
      781 gcacgatgtc cgcccaggtc gaggcaatca taaaatgttc catctgcctg atgcccaacg
      841 agcgcgcgtc gcccataaaa aacgcgccta aattcgaatc ttcgggcgca ttctgcaaaa
      901 ttttcgagac gcgctcgcgc gcctgcggcg tcaattgccg ccacgcgata taagcggtcg
      961 ttttatgccc gacatcgtcc caaccgaaag cggaaaacgg caaaatcaaa caaatcaaaa
     1021 acaaaacggt agcgaaattt ttcagcattt taggttttct cctgaatctt ctgggaagat
     1081 tttagcggag ttatggaatt agaaaaagtc ggagcggaag ttttcttttt ccgcccggcg
     1141 ctttgcaggg atttgtcaat tgcccgccgc aggcaaattt tatttgaaat aattcatcaa
     1201 ataaacagca aaaccccttg cgcgaagcgt ctaatagaca cgccgcacgg gaatgcgagc
     1261 gaggtaacaa ggagtcaaat taaaatgcag gcaattttag aacaaaatta tatcgccgac
     1321 gcgctgatta atcgcaccag cgacgctttc ggcagcaaga ttggaatcgt agcgaagatt
     1381 ttcggctgct ggcacaagga attgagccgc ccgtttgcgg ataaaaacaa atcgtatcgc
     1441 gcctgtcttc attgcggcgc gcgcaaaaga ttcgacgccg aaactttaaa aactttcgga
     1501 ccgttttact atcccccagc aatatcgccg gtcaaaagcg ggcgattaaa ctaatcccga
     1561 ccttttttcc ttcctgaagc tatacctacc ggcgcggcgc aaatgccgcg catttttttg
     1621 cgaaaaattc tattgccgaa tcaaatgctt tgctttataa atatttctaa aagaatttga
     1681 gagccaaaaa aggagaaaca atgggctggt tattattcat tatctgcatc gctccgcttt
     1741 atcttctcta taatacggga cagccaatta tttgggtaat aactctcgta aatgcaattg
     1801 ctaatttatg gtcatatggc gtaatgcaca attacgctgt tcaatcatct gctaaaaaaa
     1861 tcaaacagtt gcaagaaaac ttagctcttg aagggagact taataatcaa aagcagagtg
     1921 aaattgatag acaaaaactg gacattatct tgcaagccgt accgaattgg ctcgctaacg
     1981 taaatatact aacttttttg ataggtatcg gttttcttgg ttacggaatt tggttgttaa
     2041 tataaataca ccaaaagcct tcgcttgaaa tttcgagcga aggcttttta atttctcgtt
     2101 taccttttgg a
//
LOCUS       DASWNE010000203         3225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_571462, whole genome shotgun sequence.
ACCESSION   DASWNE010000203 DASWNE010000000
VERSION     DASWNE010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571462"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..396
                     /locus_tag="VGB68_04145"
     CDS             157..396
                     /locus_tag="VGB68_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048452.1"
                     /translation="MSKKLGQRFIVLFGLIFLLACSVLVLPDSQRAAKLDDCALCNAS
                     CNDDYNECISMGEKPWRCSQWRMACFNYCQTEVCN"
     gene            complement(465..956)
                     /locus_tag="VGB68_04150"
     CDS             complement(465..956)
                     /locus_tag="VGB68_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048453.1"
                     /translation="MSPKILFLLAFLILPAAVAAQKKEFIGYRHKGVVYGETLSNGAR
                     DLGGGLLSDENYGVSRFSKGRKQMLWLEKITSRDKKGVPGWEVLDVLVIEKPKKNQVF
                     LFSYGSSCTQNGAENLDLIVLAELQPAKKIYKVLEAWEADLVREKFDKISVEGIKCEY
                     VEP"
     gene            complement(953..1585)
                     /locus_tag="VGB68_04155"
     CDS             complement(953..1585)
                     /locus_tag="VGB68_04155"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY0048454.1"
                     /translation="MHCQLNENFHKLCLEAQNCRKCPDLTGRIAVLSELNGNLAPKVF
                     FLAEAPGRQGGNRTRTPFSGDKSGANFQILLDSINLRREEIFITNSALCNPHTATGAN
                     RKPSKTEIKNCSGFLRKTIEIINPKIIVTLGGVALEALKMIEYHQFTLKLNAAEILDW
                     NGRILLPLYHPSPQVAASHRRMHEQLADFQALAGAIEKISSLEKEQREVL"
     gene            1650..2735
                     /locus_tag="VGB68_04160"
     CDS             1650..2735
                     /locus_tag="VGB68_04160"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEY0048455.1"
                     /translation="MSQLTEQVVLDALKQIIDPDLRKDIVTLGFIRDLAITGGAVSFR
                     IVLTTPACPVKEEMETAAHEIVSALEGVSTVNVTMDAEVPKGRGIANNIAVPGVKNII
                     AVSSGKGGVGKSTVAVNLAVSLALDGAKVGLMDADVYGPNVPLMLGVADAQPQVAMNQ
                     LLPIKAHGVKMISMAVLAPPDKPMILRGPMLHGIVRQFLSDVNWGELDYLIVDMPPGT
                     GDVQLSLAQLVPVQGAVLVTTPQNVSIADVLRALKMFEQVAVPVLGVIENMSYFIPPD
                     MPDKRYNIFGEGGGRKLADEHGVPFLGEVPLGMEVRESGDKGVPVVISKPDSLQAIAF
                     RKVAEEVARQVSIEAMKPELVILSRAK"
     gene            2804..3160
                     /locus_tag="VGB68_04165"
     CDS             2804..3160
                     /locus_tag="VGB68_04165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEY0048456.1"
                     /translation="MSAVAAPTVEINLTANAAVEIKKFLAGEEDLPETAGLRVRVVPG
                     GCSGFQYSLNIEEESRNGDFIVDQHGVKLFVDMFSAQYLNGITVDYTSNMMGSGFTFE
                     NPNATGGCGCGTSFSA"
BASE COUNT          789 a          784 c          825 g          827 t
ORIGIN      
        1 aatgacgagc ggaaaaatct tgacagttcg gcaaaataga ataaataatc attttacctg
       61 cgccgccttt ttctatccgt aaattggata atttacggct caattcacaa ttaaccgctg
      121 atcctgagga ttagctcaca actaaaggag agaaatatgt cgaagaaatt gggtcaacgt
      181 tttatcgttc ttttcgggct gatatttttg ctggcgtgtt cggttctggt tttgccggat
      241 tctcaacgag ccgccaaatt agatgattgc gcgttgtgta atgcgagctg caacgacgat
      301 tataatgagt gcatcagcat gggcgaaaaa ccctggcgat gcagtcaatg gcgaatggct
      361 tgctttaatt actgccagac ggaggtttgc aattaaaggt tattaacgag tttttaaaag
      421 ccgccgcatt tgattatgcg gcggctttta tattttcaga gcgtctacgg ctcgacgtat
      481 tcgcatttga ttccttcgac cgaaatctta tcgaattttt ctctcaccaa atcggcttcc
      541 caggcttcca gaaccttata aattttcttt gcgggctgaa gttccgccag gacaatcaaa
      601 tccagatttt ccgcgccgtt ctgcgtgcag ctcgaaccgt aagaaaacaa aaacacctga
      661 ttttttttcg gcttttcgat gaccagaacg tctaaaacct cccagccggg aacgcctttt
      721 ttatcgcgcg aagtgatttt ttcgagccag agcatctgtt ttctgccttt cgaaaaacgc
      781 gaaacgccgt aattttcgtc cgacagcaaa ccgccgccca aatctctcgc gccgttcgat
      841 aaggtttcgc cgtagacgac gcctttgtgc cggtaaccga taaattcttt tttctgtgcc
      901 gcaaccgccg ccggcaaaat caaaaatgcg agtaaaaaca atatcttcgg actcataaaa
      961 cttccctttg ctctttttcc aacgatgaga ttttttcaat cgctccggct aaagcctgaa
     1021 aatctgcgag ctgttcgtgc atccggcggt gcgaagcggc aacctgcggg ctcggatgat
     1081 aaagcggcag caaaattcgc ccgttccaat ctaagatttc cgccgcgttc agcttgagcg
     1141 taaattgatg atactcaatc attttcaaag cctcaagcgc tacgccgcca agcgtcacaa
     1201 ttattttcgg attgatgatt tcaatcgttt tccgcaaaaa accggagcag tttttgattt
     1261 cggttttcga cggtttgcgg tttgcgcccg tcgccgtgtg cgggttgcac aaagcggaat
     1321 tggtgataaa aatttcttcg cgccggagat tgattgaatc gagcagaatc tgaaaattcg
     1381 cgcccgattt atcgcccgaa aaaggcgtgc gcgtgcggtt tccgccctgt ctgcccggcg
     1441 cttcggcgag aaagaaaact ttcggagcga gattgccgtt taattcgctc aaaacggcga
     1501 tgcgcccggt caggtcgggg catttccggc aattttgcgc ctcaaggcaa agtttgtgaa
     1561 aattttcgtt tagctgacaa tgcattttgc atctaattat gtaaaatgaa aggttatcct
     1621 atcaattaat tttttaatcg caaacttata tgagccaatt aacagaacag gtagttttag
     1681 acgcattaaa acaaattatc gacccggatt tacgcaaaga tatcgtgacg ctcggcttta
     1741 tccgtgattt ggcgataacg ggcggcgcgg tttcttttcg catcgttctg acgactccgg
     1801 cgtgtccggt caaagaggag atggaaacgg cggcgcacga aatcgtctct gctctggaag
     1861 gcgtctcgac ggttaatgtc acgatggacg ccgaggtgcc gaaaggtcgc ggcatcgcca
     1921 acaacatcgc cgtgccgggc gtaaaaaata ttatcgccgt ctccagcggc aaaggcggcg
     1981 tcggcaaatc gacggttgcc gtcaacctgg cggtctcgct ggcgctggac ggcgcgaaag
     2041 tcggtctgat ggatgcggac gtctacggtc cgaacgtgcc tctgatgctc ggcgtcgccg
     2101 acgcgcagcc gcaggtggcg atgaaccagc tgctgccgat caaggcgcac ggcgtaaaaa
     2161 tgatttcgat ggcggttctg gcgccgcccg ataaaccgat gattctgcgc ggtccgatgc
     2221 tgcacggcat cgttcgacag tttctttcgg acgtcaactg gggcgagctc gattatctaa
     2281 tcgtcgatat gccgccggga acgggcgacg tgcagttgtc gctcgcgcag ctcgttccgg
     2341 tgcagggcgc ggttctggtc acgacgccgc agaacgtttc gattgcggac gttctgcgag
     2401 ccttaaaaat gttcgagcag gtcgccgttc ccgttctcgg cgtcatcgaa aatatgtcgt
     2461 atttcattcc gcccgatatg cccgacaaac gctacaacat tttcggcgaa ggcggcggca
     2521 gaaaactggc ggacgaacac ggcgttccgt ttttgggcga agttccgctc ggaatggaag
     2581 tccgcgaaag cggcgacaaa ggcgttccgg tcgtcatttc caagcccgat tcattgcagg
     2641 caatcgcttt cagaaaagtc gccgaagaag tcgcgcggca ggtttccatc gaagcgatga
     2701 agccggaact tgttatttta tccagagcaa agtaaaattg aaattaatgt aaagattcat
     2761 ttaatatatg aatccaacgc tttgataaag gagaaataaa aagatgtcag cagttgctgc
     2821 accgacagtt gaaattaatt taaccgccaa cgcggcggtg gaaatcaaaa agtttttggc
     2881 gggcgaggaa gatttgccgg agacggctgg tctgcgcgtg cgcgtcgttc cgggcggctg
     2941 ctccggcttt caatacagcc tgaatatcga agaagaatcg cgcaacggcg attttatcgt
     3001 cgaccagcac ggcgtaaaac ttttcgtcga tatgttttcc gcgcagtatc tgaacggaat
     3061 tacggtcgat tacacttcga atatgatggg ctcgggcttt acgtttgaaa atccgaacgc
     3121 gaccggcggc tgcggctgcg gaacttcatt ttcggcttaa aatttaaacg gcttttataa
     3181 aaattcgaga tgcgccgcct gcaaaatcac gggcggcgca atttt
//
LOCUS       DASWNE010000204         3377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_465544, whole genome shotgun sequence.
ACCESSION   DASWNE010000204 DASWNE010000000
VERSION     DASWNE010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465544"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VGB68_04170"
     CDS             complement(<1..525)
                     /locus_tag="VGB68_04170"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0048457.1"
                     /translation="MSKKVVYVGKDLEAMSFAVNYHQWILDEFRHLLGKHVVEVGAGT
                     GSFSEMVLETKPETLALVEPSDLYNDLTANVSQNGGGTKIDFYNSIFASAAAEITAKQ
                     KPDSIIYVNVLEHIEDDNHELKLIYDSLSDGGRCFLFVPALMALYGEFDRKLGHFRRY
                     TKKELEQKCQAAGFK"
     gene            complement(565..2190)
                     /locus_tag="VGB68_04175"
     CDS             complement(565..2190)
                     /locus_tag="VGB68_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048458.1"
                     /translation="MNFFESSNARLFIFFLLLSVFTCLPIWAVDYFINQDGSGHVYSA
                     ALMTGLLKGNPAVSEIYAFNSISFPNSSGHWILIFLLLFFSPFTVTKIIVTFTYLGLV
                     AAAGWLRWLTAGRQGVATSLLIGAAIGFNWLWLMGFYNFTIGVIGFTFTLGLFYRWRE
                     NMNLTRALLLAALLVLVYFSHIIAFGILAGSICLLSVFVPPSHWKKTLGWTALAFLPI
                     VPLLIIYKLLSEAGGEFSPVWRNLSDPYSISSWITQLRSADLFVIISRRTFPFTNANS
                     NVFAVFTPGLWLLGGIFFLTAATWFSFRMQTDFFKKYSAFAVLFGVCIFIAMFAPDDF
                     QLTHGGVLRERFLLCGLIAFVPLFRTDNSVWLKRCAQFCLAVVIVFQTLALWEYSLSS
                     DRLAKDLVAAQSAVGDSTRLVSVSILEDGLRYHSLPTGQMDNYLGIGKNVQVWDNYEI
                     GHYLFPIVAKEISHRQFVLGYTGSNVFALNDKSVNIDEKLLKLEALLEANNNKIDTVL
                     VWGRDARVEAVLGKWFETEPYFQSGEVRLFRLR"
     gene            complement(2210..3094)
                     /locus_tag="VGB68_04180"
     CDS             complement(2210..3094)
                     /locus_tag="VGB68_04180"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEY0048459.1"
                     /translation="MIAQKIEITWLNLIRLYTFYFPVNKGKWRASAFARKLCRHLPEK
                     ILATTKDGRRLYVTLSDWVGESIFFLGKHEQYSAEIFARHIKKGFVCLDVGANIGWHT
                     TLMSRLSGAEGEVHAFEPVPPTFEDLKKNVSLNGEGAEVFLNNFGLGNKESTAEIHLF
                     KDLPSGHATLAGNDKKASTVFPIKIKTLDSYLEEKNIKRVDFIKVDIEGAEMMFLKGA
                     EKIFEQEIAPVIYMEMTLETSAEFGYLPNDLIVFLKSKASYRFFRIDEAGEKLVEIEG
                     FAPDDIGANVLCLPQNFD"
     gene            complement(3123..>3377)
                     /locus_tag="VGB68_04185"
     CDS             complement(3123..>3377)
                     /locus_tag="VGB68_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048460.1"
                     /translation="ALYGLRGKGGKNEPVHPDHVTYFSYSTLSLMLKREDLRVKEFCF
                     YDLGPEHRQHNPWYYNFINDVGVKISPQLADGVIAVCQLS"
BASE COUNT          982 a          849 c          721 g          825 t
ORIGIN      
        1 cttaaagcct gccgcctgac atttttgctc aagttccttt ttggtatacc ggcggaaatg
       61 cccgagcttc cggtcaaact cgccgtagag agccatcagc gccggaacaa acaaaaaaca
      121 gcgcccgccg tcgcttaacg agtcgtaaat taatttcaat tcgtgattat cgtcctcgat
      181 atgctctaaa acattaacat agattatcga atcgggcttt tgtttcgccg taatttccgc
      241 cgccgccgat gcaaatatcg aattgtaaaa atctattttc gtgccgccgc cgttttgtga
      301 aacattcgcc gtcaaatcgt tatacaaatc cgaaggctcg accagcgcca gcgtctccgg
      361 ctttgtttcc agaaccatct ccgaaaacga gccggttccg gcgccgactt cgacgacgtg
      421 tttccccaaa agatgtctga actcgtctaa aatccattgg tgataattaa cggcaaacga
      481 catcgcttcc aaatctttgc cgacataaac aactttttta ctcatctgaa attaattggt
      541 gacgtttgat tttaacagtt ttctttatct gagacgaaac agtcgaacct ctccgctttg
      601 aaaataaggc tcggtttcga accatttgcc caaaaccgct tcgacgcgcg cgtctcttcc
      661 ccagacgagc acggtatcga ttttattatt atttgcttcg agcaaagctt ccagcttcaa
      721 aagcttttca tcgatgttga cgcttttatc gtttaaggca aaaacattac tgcccgtgta
      781 gccgagcaca aactgccgat gcgaaatctc ttttgccaca atcggaaaca ggtaatgacc
      841 gatttcgtaa ttgtcccaga cctgcacgtt tttgccgatg ccgagataat tatccatctg
      901 tccggtcggc agcgaatgat aacgcagacc gtcttcgaga atactgacgg aaacgagcct
      961 cgtgctatcg ccgaccgcag attgagcggc aaccaaatct ttcgccagtc tatcggaaga
     1021 cagggaatat tcccacaacg cgagcgtctg aaaaacaatc acgacggcta aacaaaactg
     1081 cgcgcaacgc tttagccaaa cggagttatc tgttctaaat aaaggaacaa acgctatcaa
     1141 gccgcacaat aaaaatcttt cgcgcaaaac gcctccatgc gttaattgaa agtcgtccgg
     1201 cgcaaacatc gctatgaaaa tgcaaacgcc gaacaaaacc gcaaaggcgg aatatttttt
     1261 aaaaaagtcg gtttgcattc gaaaagaaaa ccaagtcgcc gccgttaaaa aaaatattcc
     1321 gccgagcagc cataaacccg gcgtaaagac ggcgaaaaca ttcgagttgg catttgtaaa
     1381 cggaaaagtc ctgcggctga tgatgacaaa taaatccgcg cttcttaact gtgtaatcca
     1441 gcttgaaatc gaatacgggt cgctcaaatt tcgccagacc ggcgaaaatt cgccgcccgc
     1501 ctcgctcagc aatttataaa tgattaaaag cggcacaatc ggcagaaaag ccagtgccgt
     1561 ccagccgaga gtttttttcc aatgcgacgg cggcacaaaa acgctcaaca gacagatgct
     1621 tccggctaaa attccgaaag cgataatatg actgaaatag accagaacca gaagcgccgc
     1681 caacagcagc gcgcgcgtca aattcatatt ttcgcgccag cgataaaaaa gtccgagcgt
     1741 gaacgtgaaa cctataacgc cgatggtgaa attataaaag cccatcagcc agagccagtt
     1801 aaaaccgatt gccgcgccga tcaaaagaga tgtagcgacg ccctgccgac cggcagtcag
     1861 ccagcgcagc cagccggctg ccgcgaccaa gcctaaatag gtaaatgtta caataatttt
     1921 agttacggta aacggagaaa agaaaagcag aagaaaaatc aaaatccagt gtccgctcga
     1981 attcggaaag gaaatcgagt tgaaagcata aatttccgaa accgccggat tgcctttcaa
     2041 aagtccggtc atcaaagccg cgctgtaaac gtgtcccgaa ccgtcctgat tgataaaata
     2101 atcgaccgcc caaatcggaa gacaggtaaa aaccgacagg aggagaaaga aaataaacag
     2161 acgggcattt gacgattcga aaaaattcat acttcggata atattcagat taatcgaaat
     2221 tttgcggcag acataaaaca tttgccccga tgtcgtccgg cgcaaatcct tctatttcaa
     2281 cgagtttttc tccggcttca tcaatccgaa aaaaccggta gctcgctttg cttttcagaa
     2341 aaactatcaa atcgttcggc agataaccga attcggcgga cgtctcgagc gtcatttcca
     2401 tataaatcac gggcgcgatt tcctgctcga aaattttttc cgcgcctttt agaaacatca
     2461 tttcagcgcc ttcgatatcg actttgataa aatcgacgcg tttgatattt ttttcttcca
     2521 gatacgaatc gagcgttttt attttaatcg gaaaaacagt cgacgccttt ttgtcattac
     2581 cggcgagcgt ggcgtgaccg ctcggtaagt ctttgaaaag atggatttcc gccgtcgatt
     2641 ctttattgcc gagcccgaaa ttgttaagga agacttcggc gccttcgccg tttagggaaa
     2701 cgtttttttt taaatcttcg aatgtcggcg gaaccggctc gaaagcgtgg acttcgcctt
     2761 ccgcgccgct cagacggctc atcaaagtcg tgtgccagcc gatatttgcg ccgacgtcca
     2821 gacagacgaa gccttttttt atgtgccggg caaaaatttc ggcgctgtac tgctcgtgtt
     2881 tcccaagaaa aaagatagat tcgccgaccc aatccgacaa tgtgacatac aaacgtctgc
     2941 cgtctttcgt cgtcgccaga attttttccg gcagatgacg acagagcttt ctggcaaaag
     3001 ccgaagctcg ccacttgccc ttgttgacgg gaaaataaaa tgtataaagc ctgattagat
     3061 taagccatgt gatttctatt ttttgagcaa tcacgcgttt tcttcctgaa aataaaaata
     3121 atttaactga gctggcaaac cgctatcacg ccgtcggcta attgcggcga aattttcacg
     3181 ccgacatcgt ttataaaatt ataataccag ggattatgct gcctgtgttc cggtcccaaa
     3241 tcgtaaaagc aaaactcttt aacccgtaaa tcctcgcgtt tgagcatcag gctcagcgtc
     3301 gaatacgaaa aataagtgac gtggtcggga tgcacaggct cgttttttcc gccttttccg
     3361 cgcaacccgt agagggc
//
LOCUS       DASWNE010000205         2068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_111945, whole genome shotgun sequence.
ACCESSION   DASWNE010000205 DASWNE010000000
VERSION     DASWNE010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111945"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..>2068
                     /locus_tag="VGB68_04190"
     CDS             85..>2068
                     /locus_tag="VGB68_04190"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HEY0048461.1"
                     /translation="MSTVFEPVATGTFVAKNGKTNGKGASAKTEYKPIGLNGQKVVSK
                     RYSLKDANGNPLEAWEDIVRRVVGHVSGAEQDFMKRELFYNAMLEVMLKREFVPNTPC
                     LVNAGKPQGQLAACFVLNVPDSINAIMEHAQAAAIIHQTGGGTGMTYEFLRPAGAMVN
                     STRGVASGPVSFMNIVNTMTEVVKQGGVRRGANMGMMRVTHPDVLRFIHAKNDQHSLT
                     NFNISVNVTDKFMEAVDRKQWFQTEFAGEKWTQAVYDPVTGKDYVVYRRPNGESATFA
                     DKQAFEAADLSDCTIEAPPQEGMIYAPDIWNRIIASAHKYAEPGIAFIDEVNRHNHMM
                     NSMGPIYASNPCGEQFLHFSNSCNLGSIDLSKFYKKQGDGTNPDECVEWDRLANVTHL
                     CTQFLDNVIDTGHFPLAEIDDVVKRTRPVGLGIMGFADLCLKHKITYGEAASIALMEK
                     VMGFVRRESWRASLKLGAEKGAFPEFEANREAYTRFLRDEIGIPEDVAVTPRNYEVTT
                     IAPTGTISLVAETSSGIEPNFSWAYVRQDTIGTRTYVHTLAAEALGIAVDQTDEESIK
                     KAAQFVVENQDKLPSYFISALDITSKQHVQVLAAAQKHVDNSVSKTCNGAKDDTIESV
                     DELYRLAKDLGCKCVSYYRDGSREGQVLTTMKTEDKK"
BASE COUNT          529 a          547 c          578 g          414 t
ORIGIN      
        1 ccaagtaatg caagaacaat tataaaagag taaaattaaa gattgagccg cgcctcaaag
       61 tgcggcattt ggagaggtga gtgaatgagt acagtttttg aacctgtcgc aacgggcact
      121 tttgtcgcca aaaatggaaa aaccaacggg aaaggcgcgt cagccaaaac ggaatacaag
      181 ccgatcggct taaacggtca aaaggtcgtc agcaaacgct acagcttgaa agacgccaac
      241 ggcaatccgc tcgaagcctg ggaagacatt gtgcgccgcg tcgtcggtca cgtctccggc
      301 gccgagcagg atttcatgaa acgcgagttg ttctacaacg cgatgttgga ggtgatgctc
      361 aagcgcgaat tcgtgccgaa cacgccgtgt ctggtcaatg cgggcaagcc gcagggacag
      421 ctcgccgcct gtttcgtgct gaacgtgccg gattccatca acgcgattat ggaacacgcc
      481 caggcggcgg caatcattca ccagacgggc ggcggaaccg ggatgactta cgaatttctg
      541 cgtccggcgg gcgcgatggt caattcgacg cgcggcgtcg cctccggtcc ggtttctttt
      601 atgaacatcg tcaacacgat gaccgaagtc gtcaagcagg gcggcgtccg gcgcggcgca
      661 aatatgggaa tgatgcgcgt cacgcacccg gacgttctgc gttttatcca cgcgaaaaac
      721 gaccagcact cgctgaccaa tttcaacatc tcggtcaacg tcaccgacaa gtttatggaa
      781 gcggttgacc gcaaacagtg gtttcagacc gaatttgcgg gcgaaaagtg gacgcaagcc
      841 gtttacgacc cggtaaccgg aaaagactac gtcgtttatc gtcgtccgaa cggcgaaagc
      901 gcgacttttg ccgacaagca ggcttttgaa gccgccgacc tttccgactg cacgattgaa
      961 gcgccgccgc aggaaggaat gatttacgcg ccggacatct ggaatcgcat catcgcttcg
     1021 gcgcataaat acgccgagcc gggaatcgct tttatcgacg aagtcaatcg tcataaccat
     1081 atgatgaact cgatgggacc gatttacgcg agcaatccgt gcggcgagca gtttctgcat
     1141 ttttcgaatt cctgcaatct cggctcgatt gatttatcga agttttataa aaaacagggc
     1201 gacggcacga acccggacga atgcgtcgaa tgggacagat tggcaaacgt cacgcatctg
     1261 tgcacgcagt ttctggataa cgtcatcgat accggacact tcccgctcgc cgaaatcgac
     1321 gacgtggtga aacgcacgcg ccccgtcggc ttgggaatta tgggatttgc cgatttgtgt
     1381 ctgaaacaca aaatcactta cggcgaagcc gcgtcgattg ccttgatgga gaaagtgatg
     1441 ggctttgtcc gccgcgaatc gtggagagcc agcttaaaac tcggcgcgga aaaaggcgcg
     1501 tttccggaat ttgaggcaaa ccgcgaagct tatacgcgat ttctgcgcga cgaaatcgga
     1561 attcccgaag acgttgccgt tacgccgcgc aactatgaag tgacgacgat tgcgccgacc
     1621 ggaacaatct cgctcgtcgc cgaaacttcc agcggtatcg agccgaattt ttcgtgggct
     1681 tacgttcgcc aggacacaat cggaacgcgc acttacgttc acacgctggc ggcggaagcg
     1741 ctgggcatcg ccgtcgacca gaccgacgag gaatcaatca aaaaagccgc gcagttcgtc
     1801 gtcgaaaatc aggataagct gccgtcgtat tttatctcgg cgctcgacat tacttccaaa
     1861 cagcacgtgc aggtgctcgc cgccgcgcag aaacacgtgg acaattccgt ttcaaaaacc
     1921 tgcaacggcg cgaaagacga cacaatcgaa tcggttgacg agctttatcg tctggcgaag
     1981 gatttgggct gcaaatgcgt ctcgtattac cgcgacggct cgcgcgaagg tcaggttttg
     2041 actacaatga agacggagga caagaaag
//
LOCUS       DASWNE010000206         2311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_430184, whole genome shotgun sequence.
ACCESSION   DASWNE010000206 DASWNE010000000
VERSION     DASWNE010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430184"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VGB68_04195"
     CDS             <1..311
                     /locus_tag="VGB68_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048462.1"
                     /translation="FFQTRLMANLREKQAKINPFAAIGRLWKASASLVVMMMTMVVAL
                     IALTIFAPKLNTASGAQTSSFDNYSTDMVIMNERLPGREPTNEQVFQIIYASDSNSRK
                     "
     gene            318..698
                     /locus_tag="VGB68_04200"
     CDS             318..698
                     /locus_tag="VGB68_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048463.1"
                     /translation="MNSSNKYNWQVRLAALSVFLLGFVAGALALNAYHVWFAASQPTK
                     QQRYEQIFDQLSLSDGQKTEIQKIVGETREEIQALRKESEPRVKEIRGRADERFQKVF
                     TPEQWQKFQNLRDTLRENEKNSSR"
     gene            complement(912..1775)
                     /locus_tag="VGB68_04205"
     CDS             complement(912..1775)
                     /locus_tag="VGB68_04205"
                     /inference="COORDINATES: protein motif:HMM:NF019003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
                     /protein_id="HEY0048464.1"
                     /translation="MFNTIKENIERRLGLAARETTVRQDEPIENINQWSGRYSRWRNN
                     ENLTNYEFVKNIYAPFTPMRRALPMLNLALISSAGAYIDGTDAFDVTAKDGDANFREI
                     PIEVGAEDMRYAARGYDPTAVQQDRNVQIPIERLQEYEANAVIGHLNQVWWSFNGFIP
                     NARLVAEQLAPPIIERLHRYEVQGALLVPASRLCHQSIAILARAIEQTGIPTLMLAVD
                     KGLTESVRPPRGAYYSGELGSVVGEPNWKEYQHRLLDEAIRWLETFDQPGVRKLSVAL
                     GTEVQNERGER"
     gene            complement(1781..2269)
                     /locus_tag="VGB68_04210"
     CDS             complement(1781..2269)
                     /locus_tag="VGB68_04210"
                     /inference="COORDINATES: protein motif:HMM:NF025046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEY0048465.1"
                     /translation="MALIADLRTFLIALWLGAAVFFTAAVAQSAFAVLPSRELAGAVV
                     NRTLAILNYSGIIIGLILLASSIVNTEKINRVKLRVEQGLLLFLTAACAFGQFVIGAR
                     LHNLRRQIGRPVDEIAADDPLRIAFGALHGYSVAVLAFAMLAALVVYFLMARRARNNF
                     IG"
BASE COUNT          600 a          617 c          561 g          533 t
ORIGIN      
        1 cgtttttcca gacgcgcctt atggctaacc tgcgcgaaaa acaggcgaaa atcaatcctt
       61 tcgcggcaat cggaagattg tggaaagctt cggcgtcgct ggtcgtgatg atgatgacga
      121 tggtcgtcgc gctgattgcc ctgacgattt tcgcgccgaa actcaatacg gcttccggcg
      181 cgcaaacgtc ctcattcgac aattattcga ccgatatggt gattatgaac gaacgcctgc
      241 cgggcagaga accgaccaac gagcaggttt ttcagataat ttacgcttcg gacagcaact
      301 ccagaaaata acgaattatg aacagcagta ataaatataa ctggcaggtc agactagcgg
      361 cgttgagcgt ttttctgctc ggttttgtcg ccggcgcgct tgccttaaac gcttatcacg
      421 tttggtttgc ggcttcccag ccgaccaagc agcagcgcta cgagcagatt ttcgaccagc
      481 tttcgctgtc cgacgggcag aaaacggaaa tccagaaaat cgtcggcgaa acgcgcgagg
      541 aaattcaagc cttaagaaaa gaatccgaac cgcgcgtcaa ggaaattcgc gggcgcgccg
      601 acgaaagatt tcagaaagtc ttcacgccgg agcaatggca gaaatttcag aatctaagag
      661 acacgcttcg tgaaaacgaa aagaattcga gcaggtaaaa taacgccagt ttaagaaagc
      721 ccgcgcgccc gtttaaactc taaattataa attttaaact gatttgtatg ttgttttcca
      781 aaataatcca gagtttataa tttataattt acagttggat ttcttctttt tccaggttaa
      841 tttaaaaaag gcaaaatgaa aattttaagt gttttcattt tgccttttgc ctttttactt
      901 ttgccccgga cttacctttc tccgcgctcg ttctgaactt ccgttcccaa agcgaccgac
      961 agttttctga cgcccggctg gtcgaaggtc tcaagccagc gaatcgcttc gtccagcagg
     1021 cgatgctggt attccttcca gttcggctcg ccgacgacgc ttcccagttc gcccgaataa
     1081 taagcgccgc gcggcggtcg aaccgattcg gtcaaacctt tatcgaccgc cagcatcagg
     1141 gtcggaattc cggtctgttc aatggcgcgg gcgagaatgg cgatgctttg atgacataaa
     1201 cgcgatgccg gaacgagcag cgcgccctgc acttcgtaac gatgcagcct ttcgataatc
     1261 ggcggcgcga gctgttcggc gacgagccgg gcgttcggaa taaaaccgtt aaagctccac
     1321 cagacctgat tcaagtgacc gataacggcg tttgcctcgt attcctgcaa gcgctcaatc
     1381 ggaatctgca cgttgcggtc ttgctgcacg gcggtcgggt cgtagccgcg cgcggcgtag
     1441 cgcatatctt ccgcgccgac ctcaatcgga atctctcgaa aattcgcgtc gccgtctttc
     1501 gccgtcacgt cgaaagcgtc cgtgccgtcg atatacgcgc ccgccgacga aatcaaagcc
     1561 agattcagca tcggcaaagc ccggcgcatc ggcgtaaaag gcgcgtaaat gtttttgaca
     1621 aactcgtaat tcgttaaatt ctcgttattt cgccagcgcg aatagcgacc cgaccattga
     1681 ttgatgtttt caatcggctc gtcttgccgg acggtcgtct cgcgggcggc aagtcccaat
     1741 ctgcgttcga tgttttcttt aatcgtattg aacatttgtt ttacccgata aaattatttc
     1801 ttgctctgcg tgccatcaga aaatatacaa ctaaagcggc aagcatcgca aaagccagaa
     1861 ccgcgaccga atagccgtgc aaagcgccga aagctattcg cagcgggtcg tcagccgcaa
     1921 tctcgtcaac cgggcgaccg atttgccgtc gtaaattatg cagccgcgcg ccgataacga
     1981 attgcccgaa agcgcaggca gcggtcaaaa ataaaagcaa gccctgctcg acccggagct
     2041 ttacgcgatt aatcttttcc gtattgacaa tcgagcttgc cagcaaaatc aagccgataa
     2101 taatgccgct gtaatttaaa atcgccagcg ttctattaac gaccgcgccc gccagctcgc
     2161 gcgaaggcaa aaccgcaaac gcgctctgcg cgaccgccgc cgtgaaaaaa acggctgcgc
     2221 cgagccataa agcaatcaga aaagtccgca aatctgcaat taaagccata cattcgagcc
     2281 gaaaacttat tatgagcggc aaaaatgcaa a
//
LOCUS       DASWNE010000207         7829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_606691, whole genome shotgun sequence.
ACCESSION   DASWNE010000207 DASWNE010000000
VERSION     DASWNE010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7829
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606691"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /gene="ruvA"
                     /locus_tag="VGB68_04215"
     CDS             <1..527
                     /gene="ruvA"
                     /locus_tag="VGB68_04215"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEY0048466.1"
                     /translation="TFYELGEEGSDVSLRIYTHVREDALQLFGFKSEREKQLYLKLIS
                     VQGIGAKSGISMLSGMSAEEIVTAIRTDNLARLTSIPGVGRKTAERLVIELRDKLNEF
                     TAQSALERAASGTAAQAEAPADAVFDDALSALVNLGYQRNAAEKALKQAIKDGTEISV
                     QKLLRKSLQLLAKG"
     gene            527..1312
                     /locus_tag="VGB68_04220"
     CDS             527..1312
                     /locus_tag="VGB68_04220"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0048467.1"
                     /translation="MKLEVISREPGERRFETPLLFVHGTGHAAWCWDENFLPYFARNG
                     FSAHAVSLRGHGASAGGENLKWTSVSDYVADVFRAASALPKMPVVVGHSLGGLVVGKY
                     LEKHSAPAGILFAPSPSEGMFRSGFGLQLRNPWLMTKVNFKQDYALMFSTPKLAKKFL
                     FSANADDRKIAGYAARFGKESYRAALEMIYNLPKPRKIKTPLLVIGAENDALIAPKKI
                     EKTARAFNADCRIFPAMAHDLMLESGWRAVADFMIDWLEKKIR"
     gene            1329..2090
                     /locus_tag="VGB68_04225"
     CDS             1329..2090
                     /locus_tag="VGB68_04225"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEY0048468.1"
                     /translation="MREIYKAIEEDFSVLDRRALAALVYTAVALTCIYYLGNAQKLVD
                     LFNQTRFAGGVEALFSNDKNNLPSLAYWIFVVVLFYSVVPALGIKYVLKRDLKDYGLN
                     LKIEKGFGKLYLQCSAVMLPLVYMVSLTAGFANKYPFLKVYDGEPYFGYALLIWELLY
                     FLQFFGLEFFFRGFLVHSLKPALGFYSIFVMTVPYCMIHFGKPMTESFAAIFAGIFLG
                     WLSYKNNSIWLGLLLHCTVALAMDVLALHHKGLLF"
     gene            2121..3161
                     /gene="ruvB"
                     /locus_tag="VGB68_04230"
     CDS             2121..3161
                     /gene="ruvB"
                     /locus_tag="VGB68_04230"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF000868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEY0048469.1"
                     /translation="MGNLDTIETRNGESLDEDKQFELSLRPTRLAEYIGQPKAKENLR
                     VFMKAALSRREALDHVLLFGPPGVGKTTMSLIIANEMGAELKSTAGPVIEKSGDLAAI
                     LTNLNEGDVLFIDEIHRLNPAIEEILYPAMEDYNLDIMIGQGTAARSIKLELPKFTLV
                     GATTRPGLITAPLRGRFGIVFHLDFYNIEDLQTIIRRSAEILNVEVEENGAREIARRS
                     RGTPRIANRLLRRVRDYAEVDYDGRITEEVAMDALNRMEVDTFGLDEVDRKLLLTIIE
                     KFDGGPVGLGTISASIHEEKDSIEEIIEPYLIQIGFLNRTPRGRMVTRLAYEHFGLDS
                     LLKISSAPSLFD"
     gene            3222..3995
                     /locus_tag="VGB68_04235"
     CDS             3222..3995
                     /locus_tag="VGB68_04235"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0048470.1"
                     /translation="MRSILLIPFLLYLGLALYAYFFSERLIFQPQFSSYRDDAGIIKL
                     TGSDGVKISARLYENPSASHTILFSHGNAEDIGRSDFIVREFQKNGFAVLAYDYHGYG
                     TSGGTATEENVYRDIDAAYDYLIGALKIPPEKIIVYGRSLGGAVSIDLASRRKAGGLV
                     VESTFVTAFRVLTKVPIFPFDKFRNIKKIKNVKCPVLFVHGRKDTLIPVWHGEKLFAE
                     ANEPKFSFWIDEADHNDVSSVGAESYLRKIRDFADNLPK"
     gene            4027..5718
                     /locus_tag="VGB68_04240"
     CDS             4027..5718
                     /locus_tag="VGB68_04240"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HEY0048471.1"
                     /translation="MKKFILSLAFVLFVLSAQAFAQNRFEGYNIFLDAPETHRQSICA
                     TRYVPPTTDIIISDLNPATPMNVKPCGGNTGARVSQSSSSTAVANASQTDYKWCFEGE
                     DKRYRISFKGDQFSGNKTYVYDWIATPEAKNLGNYNVRDFGAAGDGRTDDTIAFQSAL
                     AFMTVHNGGILNVPEGDYVVSAPLTLASGITIQGVNGLPTSYPTNNVNVKNPTRIRLS
                     GTRRALFRIGECTYKVALKNLELYADSNDGTYGVEAVGGYTASQDISFEQVSFSLLNR
                     GIYVHGLEHVNFAWQFDYIKVRNCLFIYNRDAGIYVKARNSGWTIEGTFFMNPKRQPG
                     QNADSIYMAHASGLLVQNTYSGGFAGSIGGTFLNIVDCGNITVIQSQAEAMTNAFVYN
                     EPEFPYHGDYSYPIVFINNSFGDRVDFRGRRTFISTGNQYLGNTFKAGPELRVYSTGD
                     RFCYDGTTLGCGNTAKVSGNIDRSTVFDRATVVFMTGQPDDSKGVKGHPTFFGTEVQF
                     GAPVQMPSLLQNQLPQGKSNGSMVYCSNCRRSTTPCQAGGAGAPAMMVGGQWSCL"
     gene            complement(5880..6386)
                     /locus_tag="VGB68_04245"
     CDS             complement(5880..6386)
                     /locus_tag="VGB68_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048472.1"
                     /translation="MKKQTIITLFVLITLGIFSISAMAQQATHSVWTNDNGLVIRDAE
                     TGCDYTSLRAFDMATSWSEPAPPTYPAIAHFRGVSKAYGTSRGCDDHSYDFRLIKTYA
                     TTGDDITGIWNVHRDNTIVCNLCTGTAYGLSQPAGAGNYYKVYIDDPIYGPQTWLYSG
                     YIDARKDF"
     gene            complement(6907..7410)
                     /locus_tag="VGB68_04250"
     CDS             complement(6907..7410)
                     /locus_tag="VGB68_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048473.1"
                     /translation="MKKQTIATFLVLMMLGIFSISAAAQSTHSVWTNNNGLYVRDATN
                     GCLYSSHSGFNMATSWTEPAPPLYPAVAHFRGVTSAIGFSVTCPTRSYEFRLVKTAST
                     TVDNITGAWNVYRNGVLMCNACNGVAYGLSQPAGVNNYYKVYVDDPFYGAQTWLYSGY
                     IDARKDF"
BASE COUNT         1882 a         1962 c         2060 g         1925 t
ORIGIN      
        1 ctacttttta tgagctgggc gaagaaggct cggacgtctc tctgcgcatt tacacgcacg
       61 ttcgcgaaga tgctctgcag cttttcggtt ttaaatccga gcgcgaaaag cagctttatc
      121 tgaagctgat ttccgtgcag ggcatcggcg ctaaatccgg catctcgatg ctttcgggaa
      181 tgagcgccga ggaaatcgtg acggctatca gaacggacaa tctggcgcgg ctgacctcga
      241 ttcccggcgt cggcagaaaa accgccgagc gtctggtcat cgagctgcgc gataagctca
      301 atgaatttac tgcgcagagt gcgctcgaac gagccgcttc cggcacagcc gcgcaagcgg
      361 aagcgcccgc cgacgcggtt ttcgacgacg cgctctccgc gctggtcaat ctcggctacc
      421 agcgcaacgc ggcggagaaa gccttgaaac aggcgataaa agacggcacg gaaatcagcg
      481 tccagaagct tttgagaaag agcctgcagc ttctggcgaa aggctgatga aacttgaagt
      541 aatcagcaga gagccgggcg agcgccggtt tgagacgccg ctgctgttcg ttcacggcac
      601 ggggcacgcc gcctggtgct gggacgaaaa ctttctgccg tactttgccc gaaacggttt
      661 cagcgcgcac gccgtcagcc tgcgcggaca cggcgcgagc gcgggcggcg agaatttaaa
      721 atggacttcc gtttccgatt atgtcgccga cgttttccgc gcggcttcgg cgctgccgaa
      781 aatgcccgtc gtcgtcggac attcgctcgg cggtctggtc gtcgggaaat atctggaaaa
      841 acactctgcg cccgccggaa ttttatttgc cccgtcgcct tccgagggaa tgtttcgttc
      901 gggtttcggc ttacaactgc gaaacccgtg gctgatgacg aaagtaaatt ttaagcagga
      961 ttacgccctg atgttttcga cgccgaagct ggctaaaaaa tttttgttct cggcaaatgc
     1021 cgatgaccga aagattgccg gatatgcggc gcgtttcgga aaggaatcgt atcgcgcggc
     1081 gctggagatg atttacaact tgccgaaacc gcgaaaaatt aaaacgcctc tgctggttat
     1141 cggcgcggaa aacgacgctt tgattgcgcc gaaaaaaatc gagaaaacgg cgcgcgcctt
     1201 taacgccgat tgccggattt tccccgcgat ggcgcacgat ttgatgctcg aaagcggctg
     1261 gcgggcggtc gccgatttta tgattgactg gctggaaaag aaaattcgat aaagccgatt
     1321 ccgaataaat gcgcgaaata tacaaagcca tcgaagaaga tttttccgtg ctggacaggc
     1381 gcgcgctggc ggcgctcgtc tacacggcgg tggcgctgac gtgtatttat tatctcggaa
     1441 acgcgcagaa actcgtcgat ttgtttaatc aaacgcgatt tgccggcggc gtcgaagctt
     1501 tgttttcaaa cgataaaaac aatctgccgt cgcttgccta ctggattttt gtcgtcgttc
     1561 tgttttattc ggtcgttccg gcgctcggca tcaaatacgt tttaaagcgg gatttaaaag
     1621 attacggctt aaatttgaaa atcgaaaaag gtttcgggaa attatatctg caatgctccg
     1681 ccgtgatgct gccgctggtt tatatggttt cgctgacggc gggttttgcc aataaatatc
     1741 cttttttgaa agtttacgac ggcgagcctt atttcggtta cgcgcttttg atctgggagc
     1801 ttttatactt tctgcaattt ttcggattgg aattcttttt tcgcggcttt ctcgtgcaca
     1861 gcttgaaacc ggctttggga ttttattcga tatttgtgat gaccgtgccg tattgtatga
     1921 ttcatttcgg aaagccgatg acggaaagct ttgccgccat tttcgccgga attttcttag
     1981 gctggctgag ctataaaaac aacagcatct ggctcgggct tttgctgcac tgcacggtcg
     2041 cgctggcgat ggacgtgctg gcgctgcatc ataaaggatt attgttttag aatcagaatt
     2101 aacgatttta taaaaaacca atgggaaatc tcgatacaat cgaaacacgc aacggagaat
     2161 cgctggacga ggacaagcaa tttgagcttt ccctgcgccc gacgcgcctt gccgaatata
     2221 tcggtcagcc gaaggcgaaa gaaaatctgc gagtgtttat gaaagccgcg ctctcgcgcc
     2281 gcgaagcgct cgaccacgtt ctgcttttcg gaccgccggg cgtcggcaaa acgacgatga
     2341 gcctgattat cgccaacgag atgggcgcgg aactgaaatc gaccgccggt cccgtcatcg
     2401 aaaaatcagg cgatttggcg gcgattctga ccaatctgaa cgaaggcgac gtgcttttta
     2461 tcgacgagat tcatcgtctg aacccggcga ttgaagaaat tctctatccg gcgatggaag
     2521 attacaatct cgacattatg atcggacagg gcacggcggc gcgctcgata aagctcgaat
     2581 tgccgaaatt tacgctggtc ggcgcgacga cccgtcccgg tttgattaca gcgcctttgc
     2641 gcgggcgttt cggcatcgtt tttcatctgg atttttacaa catcgaagat ttgcagacga
     2701 ttatcaggcg ctcggcggag attttaaacg tcgaagtcga agaaaacggc gcgcgggaaa
     2761 tcgccagacg ctcgcgcgga actccgcgca tcgccaatcg cctgctgcgc cgcgtccgcg
     2821 attacgccga agtcgattat gacgggcgca ttacggaaga agtggcgatg gacgcgctga
     2881 accgtatgga agtcgatact ttcggtctgg acgaggtcga tagaaagctg cttctgacga
     2941 ttatcgaaaa atttgacggc ggtccggtcg gtctgggcac gatttccgct tcgattcacg
     3001 aagaaaaaga ttctatcgag gaaatcatcg agccttattt aattcaaatc ggttttctga
     3061 atcgcacgcc gcgcggacga atggtgacgc gtctggcgta tgagcatttc ggcttggatt
     3121 cgcttttaaa aatcagcagc gcgccgagct tgttcgatta atcgcggatg aataaaaaat
     3181 ttaaaaagcg gcaaaccggc aagccgagcc tgaagacatt aatgcggtcg atattattaa
     3241 ttcctttttt actttatctg ggattggcgc tctacgctta ttttttctcc gagcgtctga
     3301 tttttcagcc gcaattttcc tcttaccgcg acgacgcggg cattataaag ctgaccggct
     3361 cggacggcgt aaaaatttcc gcccggctgt atgaaaaccc gagcgcttcg cacacgattc
     3421 tgtttagtca tggaaacgcc gaagacatcg gcaggtcgga tttcattgtg cgggaatttc
     3481 aaaaaaacgg cttcgctgtt ttggcttacg attatcacgg ctatggaacc agcggcggca
     3541 cggcgaccga agaaaacgtt tatcgcgaca ttgacgcggc ttacgattat ctgattggcg
     3601 cgttgaaaat cccgcccgaa aaaatcatcg tttacgggcg ctcgctgggc ggcgcggttt
     3661 cgattgattt agcttcgcgc cgaaaagcgg gcggtctcgt cgtggaaagc acattcgtca
     3721 cggcttttcg cgttctgacc aaagttccaa tctttccctt cgacaaattc cgtaacatta
     3781 agaaaatcaa gaatgtaaaa tgtccggttc ttttcgttca cgggcgaaaa gacacgctta
     3841 ttcccgtctg gcacggcgag aaattgtttg ccgaggcgaa cgagccgaaa ttttcctttt
     3901 ggattgacga agccgaccac aacgacgttt cgagcgtcgg cgcggaaagc tacctgcgca
     3961 aaattcgtga tttcgccgat aatctgccaa aataaattca cataataaat tctccccgaa
     4021 aaaaatatga aaaaatttat tttaagtctg gcatttgttc tgttcgtttt gtccgctcag
     4081 gctttcgccc agaacaggtt cgagggctat aacatttttc tggacgcgcc ggaaacgcac
     4141 cgccagtcga tatgcgcgac gcgctatgtt ccgccgacga ccgacatcat tatctccgac
     4201 ctcaacccgg cgacgccgat gaacgtcaag ccgtgcggcg gcaatacggg cgcgcgcgta
     4261 tcgcagagca gcagctcgac ggctgttgcg aacgcgagcc aaaccgacta caaatggtgt
     4321 ttcgagggcg aggacaagcg ctaccggatt tcttttaagg gcgaccagtt cagcggcaac
     4381 aagacgtacg tttacgattg gattgcgacg cccgaagcga aaaatcttgg caattacaac
     4441 gtcagggatt tcggcgccgc cggcgacggg cggacggacg acaccatcgc cttccagagc
     4501 gcgctcgcat ttatgaccgt gcataacggc ggaattctga acgttcccga aggcgattac
     4561 gtcgtcagcg cgccgcttac cctcgcgtcc ggcatcacca tccagggcgt caacgggctg
     4621 ccgaccagtt acccgaccaa taacgtcaac gtcaaaaacc cgacccgcat tcgtctgagc
     4681 ggcacgcggc gcgctctttt tcgcatcggc gaatgcactt acaaagtcgc gctcaaaaat
     4741 ctcgaactgt atgccgacag taatgacggc acttacggcg tcgaagcggt cggcggttat
     4801 accgcttcgc aggacatatc gtttgagcag gtgagcttca gcctgctgaa tcgaggcatt
     4861 tacgttcacg gcttggagca tgtcaatttt gcctggcagt tcgattacat caaggtcaga
     4921 aattgtcttt ttatctataa ccgcgacgcg ggcatctacg tcaaagcgcg caattccggc
     4981 tggacaatcg agggcacttt ttttatgaac ccgaaacgcc agcccggaca gaacgccgac
     5041 tcgatttata tggcgcacgc ttcggggctt ctggtgcaaa acacatacag cggcggtttt
     5101 gccggctcaa taggcggaac ttttctcaat atagtggact gcgggaacat caccgtcatc
     5161 caatcgcaag ccgaagcgat gaccaacgcg ttcgtttata acgaaccgga atttccgtat
     5221 cacggcgatt actcataccc gatagttttt atcaataatt ctttcggcga ccgcgtggat
     5281 tttcgcggca ggcgcacctt tatctcgacg ggcaatcaat atctcggcaa cacctttaaa
     5341 gccggaccgg aattgcgcgt ttactcgacc ggcgaccgct tttgctacga cgggacgacg
     5401 ctcggctgcg gcaatacggc gaaagtcagc ggaaacatcg acagatcgac ggttttcgac
     5461 cgcgcgaccg tcgtctttat gaccggacag cccgacgact cgaagggcgt gaaaggacat
     5521 ccgacttttt tcggaaccga agtgcagttc ggcgcgcccg tccagatgcc ttcgctgctg
     5581 caaaatcagc ttccgcaggg aaaatcgaac ggctcgatgg tttactgctc gaactgccgc
     5641 cgctcgacga cgccctgtca ggcgggcggc gcgggcgctc cggcaatgat ggtcggcggt
     5701 caatggagct gcctgtaaag atgtcgaaag cgtttattat cgaagaatgg gaacagacga
     5761 atccgcgctt tcacgcgtaa caaagcgaaa caaagcgaaa aataaaaaaa gagctcaagc
     5821 ttaagccttg agcccttttc tcttgaaaac agttgaacgc cgtatttgtt caaaaaaatt
     5881 tagaaatcct tgcgagcgtc gatgtagccg ctgtaaagcc acgtctgcgg tccgtaaatc
     5941 gggtcgtcga tgtagacttt atagtaattt cccgcgccag ccggctgact cagcccgtaa
     6001 gccgttcctg tgcataaatt acacacaatc gtattatcgc gatgaacgtt ccagatgccg
     6061 gtaatgtcat cgccggtcgt ggcgtaagtt tttattagac ggaagtcgta cgaatggtcg
     6121 tcacagccgc gactcgtacc gtaagccttc gagacgccgc gaaaatgcgc tattgccgga
     6181 taggtcggcg gcgccggttc cgaccacgaa gtcgccatat cgaaagcgcg aagagaggtg
     6241 tagtcgcagc cggtttcggc gtcgcgaatc accaagccgt tgtcgtttgt ccagacgctg
     6301 tgcgtcgcct gttgagccat cgccgaaatc gaaaagattc cgagcgtgat taatacaaaa
     6361 agggttataa tcgtttgttt tttcatttaa agttcctcct aaggtcgtta tgggtcacca
     6421 aagagtctgg aagatttgca ggctttcacc ctccgtttct tctgtttcct aggtggttgt
     6481 atgtttttta aaatcgcgct ttatctcaaa gagttgtcaa agcgcagttg aaatccaggg
     6541 tagtgacggc tgctcaaatc cacaacggca acaggacggc gtgggcttaa gcaatcaaac
     6601 aaattcgcgt tcaactgaaa ttctcgttcg cgcttgcaat cacgcttggc tgagagtcga
     6661 aatttcgcct tcgataattg acgcaaaatt ttgttttgtt ttgcttaaaa ttgttaattt
     6721 tttctgattt aatttctcaa aagcgtgaga aaagatttaa ggtcggagct aaagtgttga
     6781 ggtcaatggt tttcgatgtt tgcgcccgca attaatttat ttctatcgga gaaaatggag
     6841 aattcgggat ttaaataccg aactctccaa attataatcg gtcgccgtct tcggattcgg
     6901 cgaaagctag aaatccttgc gagcgtcgat gtagccgctg taaagccacg tctgcgcgcc
     6961 gtagaacggg tcgtcaacgt agactttgta ataattgttg acgcccgccg gctggctcag
     7021 cccgtaagcc acgccgttac aggcgttaca catcagcacg ccgttacgat agacgttcca
     7081 cgcgccggta atattatcga cggtcgtcga cgccgtttta accagccgga attcatacga
     7141 gcgggtagga caggtcacgg aaaatccgat agcactggtc acgccgcgaa aatgggcgac
     7201 cgccggatag agcggcggcg cgggctcggt ccacgaagtc gccatattaa agcccgaatg
     7261 cgaagaatat aaacagccgt tggtcgcgtc gcgaacgtac aagccgttgt tatttgtcca
     7321 gacgctgtgc gttgactgcg ccgccgccga aatcgaaaag attccgagca tcattaacac
     7381 caaaaaagtt gcgattgttt gttttttcac ttgaatttcc tcctaaggtt gtggcacccc
     7441 aaaaagattg gaagattgta ggcgtttccc tccgctttct tctgtttctt aggtggtttg
     7501 ctgtcgtttg tttttaattt ggaattgtcc tcgcgcaaag acgcggctgt gggctgttta
     7561 ttcccttttg tcagccattg cagagttctc cttcaatcgg cgtgccgcag atgccggaaa
     7621 ccttaaaagc gatatttttc agtgattaaa agtttttgcg cttgagccgc gcgataaata
     7681 ttaatatcaa ttttgatatg ccgactatca attctgatat aaattaaatt gaaaatttcc
     7741 gaaggcggcg caaaaaagca aaatggagaa gaaaacctcg atttccttct ccattttgct
     7801 tttttgatgg ttgtttttat tcttcgccg
//
LOCUS       DASWNE010000208         1775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_358635, whole genome shotgun sequence.
ACCESSION   DASWNE010000208 DASWNE010000000
VERSION     DASWNE010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358635"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VGB68_04255"
     CDS             complement(<1..385)
                     /locus_tag="VGB68_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY0048474.1"
                     /translation="MKSFKSFPVSENVARMQTSSTLKAAQTAANLREQGFDVIDLTVG
                     EPDFQTPEFIRDLAIEGLQKGLTKYTPSAGMKALQESIAEFYAAQFGASFSPPEVAAA
                     CGGKQALFNAACTVLNPGDDVLIPKP"
     gene            complement(476..844)
                     /locus_tag="VGB68_04260"
     CDS             complement(476..844)
                     /locus_tag="VGB68_04260"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0048475.1"
                     /translation="MPSILRVQHAQITIPKNAEDEARRFYCGVLGLKEVPKPESLKRR
                     GGFWLEIGAFQVHVGTEDGFDRTKTKAHIAYEVEDLDGWRERLAGNGVEILEGVAIPN
                     YRRFEFRDPFGNRVEFLEAA"
     gene            complement(898..1101)
                     /locus_tag="VGB68_04265"
     CDS             complement(898..1101)
                     /locus_tag="VGB68_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048476.1"
                     /translation="MEKLKLENFLLRALIILWIISFAAILTLRLIVYRRTGVFEYGSA
                     VYALVIPGFLVLMVYYLQTRGKK"
     gene            complement(1136..1627)
                     /gene="coaD"
                     /locus_tag="VGB68_04270"
     CDS             complement(1136..1627)
                     /gene="coaD"
                     /locus_tag="VGB68_04270"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEY0048477.1"
                     /translation="MRRAIFPGSFDPLTNGHLDIIKRSLPLFDEIVIAVLNNPDKNPM
                     FSVEERCRMIEEILPEIKSGDCCLIVDSFSGLTADFARRKEATAIVRGIRAVSDYEYE
                     LRMALMNRRLEPEIETVFLMAAEEYSYVSSNLMKQVFMLGGRVEGLVPELVEKKMREK
                     LGK"
BASE COUNT          469 a          444 c          409 g          453 t
ORIGIN      
        1 agggcttcgg aatcaacacg tcgtcgcccg ggtttaatac agtgcaggcg gcgttgaaaa
       61 gcgcctgttt gccgccgcac gccgccgcga cttccggcgg cgaaaaggaa gcgccgaatt
      121 gagcggcgta aaattcggcg attgattcct gcaaagcctt cattcccgcg ctcggcgtgt
      181 atttcgtcaa gcctttctgc aagccttcga tagccaaatc ccggataaat tcgggcgttt
      241 gaaaatccgg ctcgccgacc gtcaaatcaa tcacgtcaaa accctgctcg cgcagattgg
      301 cggcggtttg cgcggctttc aaggtggaag acgtttgcat ccgggcgacg ttttcggaaa
      361 cgggaaaaga tttgaaggat ttcataattt ttagtttaaa aaacgtatta cagaattatt
      421 accgaataag aaattaaata caaaaaatta taaaatacag ggaatttatc gtcttttacg
      481 cggcttccag aaactccacg cgattgccga acgggtcgcg aaactcgaag cgccggtaat
      541 tcggaatcgc aacgccttcg agaatttcaa cgccgtttcc ggcgagcctt tcgcgccagc
      601 cgtccaaatc ttcgacttca taagcgatgt gcgctttcgt tttcgtccgg tcaaagccgt
      661 cttccgtccc gacgtgaacc tgaaacgcgc cgatttcgag ccagaatccg ccgcgccgct
      721 taagagattc cggtttcggc acttccttta agccgagaac gccgcaataa aaccggcgcg
      781 cttcatcttc cgcgtttttc ggaatcgtga tttgcgcgtg ctgaactctg agaattgacg
      841 gcatttttaa catctgaata tcatctgcga aaaatcaaat tcggcattat gtcgatttta
      901 ctttttgcct ctggtttgaa ggtaataaac catcaaaact aaaaatccgg gaatgaccag
      961 agcgtaaacc gcgctcccgt actcgaatac gccggtgcgc cggtacacta tcaggcgcag
     1021 agtgagaatc gccgcaaaag aaataatcca gaggataatt aatgctcgca acaaaaaatt
     1081 ttccagtttc aatttttcca taatctttct ccggcgtctg atttttattt ccgcattatt
     1141 tgccgagttt ttccctcatt ttcttttcca ccaattccgg cacgagtcct tcgacgcgac
     1201 cgccgagcat aaaaacctgt ttcatcagat ttgacgaaac ataagaatat tcttccgccg
     1261 ccatcagaaa aaccgtctcg atttccggct ctaaacgccg gttcatcaaa gccattcgca
     1321 gctcgtattc gtaatcggaa acggcgcgaa tgccgcgcac aatggcggtc gcttctttgc
     1381 ggcgcgcaaa atcggcggtc aagccggaaa agctgtcgac gattaagcag caatcgcccg
     1441 atttgatttc gggcagaatt tcctcaatca tccggcatcg ttcttcgacg gaaaacatcg
     1501 gatttttgtc gggattgttt aaaacggcga taacgatttc gtcaaaaagc ggcaggctcc
     1561 gcttgataat gtcgagatga ccgttggtca gagggtcgaa cgaaccgggg aaaatagcgc
     1621 ggcgcatagg tttttatagt ccgaagtccg aagtcagaag tctaaagtct aaagtcggta
     1681 aatccgtatt cagtctctac gattataaaa cgaaagtcga gctatcaata caaaacgact
     1741 ttggaattgg acattgtact tttaaaattt tgttt
//
LOCUS       DASWNE010000209         2612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_536988, whole genome shotgun sequence.
ACCESSION   DASWNE010000209 DASWNE010000000
VERSION     DASWNE010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536988"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VGB68_04275"
     CDS             complement(<1..601)
                     /locus_tag="VGB68_04275"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY0048478.1"
                     /translation="MKPLLVWLILCVIWGTTWIFIKRGLDDLPPVSFAAIRFTVACLL
                     ILPALWWQKIPFPKGRAAWIIIFITGFLQFFFNYGLLFWGEQHISSGLAAVLQATIPA
                     FGLILARIYVGESITGLKIVSILLGLLGISVIFREQLTLNGQMAFLGSLAVVVGAFGA
                     SYASVLTKAKGQKLHPASMVFGQMLVGHIPLWFVGLSVEG"
     gene            complement(673..1224)
                     /gene="rsmD"
                     /locus_tag="VGB68_04280"
     CDS             complement(673..1224)
                     /gene="rsmD"
                     /locus_tag="VGB68_04280"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEY0048479.1"
                     /translation="MRIIAGKYRRRVLKSPPSLQTRPTSDRLREALFNVLAAQIDDET
                     RFLDLCSGTGAIGIEALSRGALHVTFVDKSRKSCGLIEENLDLLEIPEAETEVVQSDA
                     EEFLRKTKQTFDIVYYDPPYQTEYRDVLYLFGEPEAKLLAENGILIAEHHNKNNLPDL
                     IGELRRWRILRQGDSSLSFYERS"
     gene            complement(1381..2364)
                     /gene="rsmD"
                     /locus_tag="VGB68_04285"
     CDS             complement(1381..2364)
                     /gene="rsmD"
                     /locus_tag="VGB68_04285"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEY0048480.1"
                     /translation="MENRRHTGTAPGTSFNQNRPYRKQEDRNYGERKFQPRNDRFQSG
                     DNNTRDNNARDNNSRFQPRDERFRGKDDRFPPRENRFRSGSGTGTGATNDRDRSNERF
                     PRRDDRFQGRNDRFPPRKNFIPRGKPTEPWKQFDKPKVYDEMQVTDGKHRGKFLTSTA
                     SPKVRPTARRLREVMFRLIFRRVRAGRFLDLCAGSGAVGIEAISRGALISTFVERSGK
                     MCSFIKKNLEACGIKEGHGEICELEVVPFLKKMQKRRRFWDVVYFDPPYDTNYDEVLD
                     YLKRGVAIKPGGLLLIEHHAEMFFPEKIGVLKRWRVVTQGDTALSFYERKG"
BASE COUNT          642 a          674 c          627 g          669 t
ORIGIN      
        1 tgccttcgac gctcaagccc acaaaccaga gcggaatgtg accgaccagc atctgcccga
       61 aaaccatgct cgccggatgc agcttttgtc ctttcgcttt cgtcaaaacg ctggcatatg
      121 acgcgccgaa agcgccgacc acgaccgcca gactgcccag aaaagccatc tgcccgttca
      181 gcgtcaattg ctctctgaaa atcactgaaa tgcccaaaag accgagcaaa atcgagacta
      241 ttttcaaacc cgtaatggat tcgccgacgt agattcgcgc cagaatcaac ccgaaagccg
      301 gaatcgtcgc ctgcaaaacc gccgccagac cggaagagat gtgctgctcg ccccagaaaa
      361 gcagcccgta attaaagaaa aactgcaaaa aacccgttat aaaaattata atccacgcgg
      421 cacgaccttt cggaaacgga attttctgcc accagagcgc cggcagaatc agcaggcagg
      481 cgaccgtaaa tcttatcgcc gcaaagctta ccggcggcaa atcgtcgagt ccgcgcttga
      541 taaaaatcca ggtcgttccc cagatgacgc acaggataag ccagaccagc agaggtttca
      601 taagcgaaaa ataaaataac agattacacg aattttctgc tgattgaaaa aatagaaaag
      661 ttttttgccg ttttaactgc gctcgtaaaa gcttaagctc gaatcgccct gccttaagat
      721 tcgccagcgg cgcagctcgc caatcaaatc gggaagatta tttttgttgt gatgctcggc
      781 aatcaatatc ccgttttcgg caagcagttt agcttccggc tcgccgaaaa gatacaaaac
      841 atctcgatac tcggtttgat aaggcgggtc gtaataaacg atgtcgaacg tctgctttgt
      901 tttccgcaga aactcttcgg cgtcgctttg cacgacttcc gtctccgctt cggggatttc
      961 cagcaggtcg agattttctt cgataagccc gcaggatttt cgggatttat cgacgaaggt
     1021 gacgtgcagc gcgccgcgcg agagcgcttc gatgccgatt gcgcccgtgc ccgagcacaa
     1081 atccagaaaa cgtgtttcgt cgtcgatctg cgcggcgaga acgttaaaaa gagcttcgcg
     1141 cagacggtct gaggtcggtc gtgtctgcaa agaaggcggg ctttttaaaa cccgccttcg
     1201 atattttccg gctatgattc gcataagaga tttgcgattt tagattttag ctcggcgatt
     1261 ggattcggaa attcattttt ttgatttcca acattaattt tttaattttg cggtttcatt
     1321 ttcaaaaaat tttgcgaagc aaaatccata tccaatcgcc aatcgcaaat cgaaaattac
     1381 ctagcctttt ctttcgtaaa aactcaaagc cgtgtcgccc tgcgtgacga cgcgccagcg
     1441 tttgaggacg ccgatttttt ccgggaaaaa catctcggcg tgatgttcga ttaataaaag
     1501 accgccgggc ttgattgcca cgccgcgttt cagataatcc agaacttcgt cgtaatttgt
     1561 gtcatacggc gggtcgaaat aaaccacgtc ccagaagcgg cggcgtttct gcattttttt
     1621 cagaaaaggc acgacttcga gctcgcaaat ctcgccgtgt ccttccttga tgccgcaggc
     1681 ttcgagattt ttcttgataa agctgcacat tttgcccgag cgctcgacaa acgtgctgat
     1741 cagcgcgccg cgcgaaatcg cctcgatgcc gaccgcgccc gaaccggcgc agaggtcgag
     1801 aaaacgtccg gcgcgaacgc gtcggaaaat caatctgaac ataacttcgc ggaggcggcg
     1861 cgccgtcggg cgcactttcg gcgaagccgt gctggtcaag aattttcctc tgtgttttcc
     1921 gtctgttacc tgcatctcgt cataaacttt gggcttgtca aactgcttcc agggctcggt
     1981 cggcttgccg cgcggaataa aatttttgcg cggcggaaag cggtcgtttc tgccctgaaa
     2041 tctatcgtcg cggcgcggaa atcgctcgtt ggagcggtca cggtcattgg tggcgccggt
     2101 tcccgtcccg ctgccgctgc gaaacctgtt ttctcgcgga gggaaacggt cgtctttgcc
     2161 gcgaaaacgc tcgtctcgcg gctgaaagcg gctgttatta tcgcgcgcat tattatcgcg
     2221 cgtattatta tcgcctgact gaaacctgtc gttgcgcggt tgaaatttcc gctcgccgta
     2281 gtttctgtct tcctgttttc tgtagggtcg gttttgatta aaagatgttc ccggtgctgt
     2341 tccggtgtgt cgtcggtttt ccattttttt tcctctttca atagattggc gcgtgttcgg
     2401 ctcagagagg aacagccaaa atttcgcacg ctaatttctg ctactatcca atcgcaaatt
     2461 gtccgaacta aaattgggaa tttttgagaa aattcggtcg agcattttgc aatcaattaa
     2521 gattctctca gtttataaat gatttagaga aactgttaca gacgaatata aacgatttag
     2581 gcgaaaaaat tcaatgcttt tcatcactct tt
//
LOCUS       DASWNE010000210         1574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_321943, whole genome shotgun sequence.
ACCESSION   DASWNE010000210 DASWNE010000000
VERSION     DASWNE010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321943"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VGB68_04290"
     CDS             <1..745
                     /locus_tag="VGB68_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048481.1"
                     /translation="GGDLLTTVDDDIQVAANWFVEIEKIFRSRWTEVDFVGGKMLPDW
                     ENQQIPAWIEPLKDGVIGWRDYGEKEWRYAVDTPMLTGGHAIFKREIFDAIGLFAEGV
                     GATGKNLMSCEDDILYDKLLSAGKRGVYCPQLVVHHFVPQYRVSKSYYRQWCFGAGMS
                     WNVLDSHYKAFEGARIFGVPRYLYRQVFGDLIGKIKTVVARDESESLARENKILVFAG
                     FFYARNLKDGWLDKPLQSIVKRTVKAVQR"
     gene            891..>1574
                     /locus_tag="VGB68_04295"
     CDS             891..>1574
                     /locus_tag="VGB68_04295"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEY0048482.1"
                     /translation="MNETEQKKPSLKSQSAWLLFAKIAGFGFAFLLPLLVVRFLTQEK
                     VGVYRQVFLVIANAVSILPLGFSMSAFYFLNRETERRGAVVFNILLFNLVVGGLASLA
                     LFLFPHLLGNLFNNAEITRLAPAIGVVIWLWIFSAFLETIAVANQESRLATAFIILAQ
                     LTKTALMALAVIIFSTVESFLYAAMAQAGLQTLVLLVYLNNRFPRFWTSFDFKFFREQ
                     MFYALPFGLA"
BASE COUNT          357 a          369 c          433 g          415 t
ORIGIN      
        1 cggcggcgat ttgctgacga cggtcgacga cgatattcag gtcgccgcga actggttcgt
       61 cgaaatcgaa aaaattttcc gaagccgttg gacggaagtc gatttcgtcg gcggaaaaat
      121 gctgcccgat tgggaaaacc agcagattcc ggcgtggatt gagcctttaa aagacggcgt
      181 tatcggctgg cgcgattacg gcgaaaagga atggcgatac gccgtagata cgccgatgct
      241 gacgggcggg cacgcgattt tcaagcgcga aatttttgac gcaatcgggc tttttgccga
      301 aggcgtcggc gcgaccggga aaaatctgat gagctgcgaa gacgatattc tttacgataa
      361 attgctcagc gccggaaagc gcggcgttta ttgcccgcag ctcgtcgtcc atcatttcgt
      421 gccgcagtac cgggtcagca aatcgtatta tcgccagtgg tgtttcggcg cgggaatgtc
      481 ctggaacgtc ttggacagtc attataaagc ttttgagggc gcgcgaattt tcggcgttcc
      541 gcgctatctc tatcgacagg ttttcggcga tttaatagga aaaatcaaaa ccgtagtcgc
      601 gcgcgacgaa agcgaatcgc tggcgcgcga aaacaagatt ctggtgttcg ccgggttttt
      661 ctacgcgcgc aatctgaaag acggctggct ggataaacct ctgcaatcaa tcgtcaaacg
      721 aaccgtgaaa gcggtgcaaa gataagaaag cgaaaaaggg taaaagtgaa aaagggtaaa
      781 agtgaaaaag tgaaaaaatg aaaaagatta aaagtgaaaa agcctcgtcg tttctcgacg
      841 gcagactttt tcatttttac gccttttccg ctttttcact ttaaaaacag atgaacgaga
      901 ccgaacaaaa aaagccttcg ctaaaatcgc agagcgcgtg gctgctgttt gcgaaaatcg
      961 ccggtttcgg atttgcgttt ctgttgccgc tgctggtcgt ccgttttctc acgcaggaaa
     1021 aggtcggcgt ttacaggcag gtttttctgg ttatagccaa cgcggtttcg attttgccgc
     1081 tcggtttttc gatgagcgcg ttttattttc tgaatcgcga aaccgagcgg cgcggcgcgg
     1141 tcgtttttaa tattttattg tttaatctgg tcgtcggcgg gctggcttcg ctcgcgcttt
     1201 ttctttttcc gcaccttttg ggaaatcttt ttaataacgc ggaaattacg cgtctggctc
     1261 cggcaatcgg cgtcgtcatc tggctctgga ttttttccgc ttttctggag acaatcgccg
     1321 tcgccaacca ggaatcgcgg ctcgccacgg cttttatcat tctcgcccag ctgacgaaaa
     1381 ccgcgctgat ggcgctcgcc gttataattt tttcgacggt cgaatcgttt ctgtacgcgg
     1441 cgatggcgca ggcgggcttg cagacgctgg ttttactcgt gtatctgaac aatcgttttc
     1501 cgcgcttctg gacgagcttc gattttaaat tcttccgcga gcagatgttt tacgcgctgc
     1561 cgttcggtct ggcg
//
LOCUS       DASWNE010000211         4223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_430284, whole genome shotgun sequence.
ACCESSION   DASWNE010000211 DASWNE010000000
VERSION     DASWNE010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430284"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1657
                     /locus_tag="VGB68_04300"
     CDS             <1..1657
                     /locus_tag="VGB68_04300"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0048483.1"
                     /translation="SFQQAFNNDSKVENTYYGVFAQDDWRARSNLTVSYGLRYEREKV
                     VGDNNNFGPRASIAWNPFKGEKTVLRAGAGIFYNRVLLRTIDDFTAGAQEYYFDSRNL
                     TTAQQNAARPFLSNLFPNPRPLTLDTQIPIDAAGTTRSVRELAISGQAFRSLEPNLKI
                     PESYQFNIGFEREIFKGIVFETNLTYNKTAHLWREYNQNAPVLPAGTPDRDANGQITF
                     TDYLLGVTTGPNRFVLGSPTDTVGTMVNGGTAACTATTPLCIVNLNTRNVTDCGGSTN
                     SATCRALTTLDPLRPFFSQGITSQLERVASIGNSQYRGATFEIRNRYRKFGYGFAGSM
                     RFVYTYSKLEDDGIVNTSEATLPGDFQREWSRSLLDRRHRFAFSGTFDMPNWLGGLRV
                     SPIFRFGSSSPFNLGNGGEDRNLDDISNDRPNFSGNLDNIVWREFGSAFPAELANQFS
                     LAPIGSPGNLPRNVGKGPKLYIFDLNVSRQFKFTERFRLRPSIEFNNILNMTVFSFGS
                     NFIDFNLLNSTNPTTVANAQAGFLAPTRTVRPRQIRLGVRFDF"
     gene            1808..2572
                     /locus_tag="VGB68_04305"
     CDS             1808..2572
                     /locus_tag="VGB68_04305"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEY0048484.1"
                     /translation="MSSILEKDVPFSEISIESVLLDVDLFVKYRSAEKAFSILREAIE
                     RSPRSIPLREKMRDICVAHKNLTEAARHCLALVSLYITREDFDLAYDRLQEAKLLDPR
                     ISVAPGLEAIRRARRPEFAVHREKSSYEVRSDTAFAGDLALVSIFDAVTVVENSRLTG
                     LLIIKSDRLLGSVSFNEGKIVDAEANGHNGTTAFRDIIEVASGVFEFSTSENEFPVVI
                     QVSSNTNFLLDILTQLDTEKAEKQGMRTMSADDFPE"
     gene            complement(2642..>4223)
                     /locus_tag="VGB68_04310"
     CDS             complement(2642..>4223)
                     /locus_tag="VGB68_04310"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEY0048485.1"
                     /translation="KVMSDLPCVFISFSRADQKEVCLLISALDLQNIKCWDYSDLGQD
                     IAAGRDIKSFLAAKIDSCEYFIAVISPHSLNEEMSAYPRFEVQYALESGRHSEEKLMP
                     ILLNKPQNWQEIYPELNGIKWIEAASLNEDKLDDITCRICNSLNVKYIPPFLKDPKVF
                     FAERFLREAEHLNLPIADFNKLTKYMNKCGQMVLEEKWEKAGKIVSSLLIEPALSTPG
                     VKFYYPFILEGVFEIFSSQSKEGKLRAKGLETAERIFSEASEWQNIYDKSLLGLAFAG
                     LGQVYFLTGRYNESVDAYRRALDYMPNDEYILANLINSSCCSDANAAAALFEQIDSSD
                     LSPENKLNLIKIKGLFHYRQGEYETAVKIFQFLTLEEFDEAGALYYFLSLEKCGKDSE
                     ALSFLNSITERLKTTKLYHYLADKYSRLGQPANAICIYENFLCQPERRTRRLLIDYAR
                     VLKTVGNQNKMREVCGTILDSKNFGYSPLTSEDLFYRGYANYLLGKDNPTKYVLAEYD
                     YGSSSNFLKTGYGELEGD"
BASE COUNT         1092 a         1065 c          965 g         1101 t
ORIGIN      
        1 cagcttccag caagccttca ataacgattc gaaagtcgaa aacacttatt acggagtttt
       61 cgctcaggac gactggcgcg cccgttctaa cttaaccgtc agctacggtt tgcgctacga
      121 aagagaaaag gtcgtcggcg ataacaacaa tttcggaccg cgcgcctcta ttgcctggaa
      181 tcctttcaaa ggcgaaaaaa ccgtgcttcg tgccggagcc gggattttct acaaccgcgt
      241 tttgctgcgc acgattgacg attttacggc tggcgcacag gaatattatt tcgattcgag
      301 aaatctgacg acggcacagc aaaacgccgc gcgtcctttt ctgagcaatc ttttcccgaa
      361 tccgcgcccg ctcaccctcg acacgcagat tccgattgac gccgccggaa cgaccagaag
      421 cgtcagagaa ctggcaatct ccggtcaggc tttccgcagc ctggagccga atctgaaaat
      481 tccggaaagc tatcagttca acatcggatt cgagcgtgaa atctttaagg gaatcgtttt
      541 cgagacgaac ctgacgtata acaaaaccgc gcatctgtgg cgcgaataca accagaacgc
      601 gccggttctg ccggcgggca cgcccgaccg cgacgccaac ggtcagatta cgtttacgga
      661 ttatctgctc ggcgtgacga ccggaccgaa cagattcgtg ctcggttcgc cgaccgatac
      721 tgtcggcacg atggtcaacg gcggaacggc ggcttgcacg gcgacgaccc cgctctgcat
      781 cgtcaatctg aacacgagaa acgtcaccga ttgcggcggc tcgacgaatt cggcgacttg
      841 ccgcgctttg accacgctcg acccgctgcg cccgtttttc tcgcagggaa ttaccagcca
      901 gcttgagcgc gtcgcttcaa tcggcaacag ccaatacaga ggcgcgacgt ttgaaatcag
      961 aaaccgctat cgcaaattcg gttacggttt cgccggttcg atgcgttttg tctacacgta
     1021 ttcgaaactg gaagacgacg gtatcgtcaa cacttcggaa gcgactttgc cgggcgattt
     1081 tcagcgtgaa tggtcgcgca gcctgctcga ccgccgtcac cgttttgctt tttccggcac
     1141 gttcgatatg ccgaactggt taggcggttt gcgcgtgtcg ccgattttcc gtttcggttc
     1201 gagctcgccg tttaatctcg gcaacggcgg cgaagaccgc aacctggacg acatcagcaa
     1261 cgaccgcccg aatttcagcg gcaatctgga caatatcgtc tggcgcgaat tcggctcggc
     1321 gtttcccgcc gaattggcaa atcaattttc gctcgcgccc atcggaagtc cgggcaatct
     1381 gccgcgcaac gtcggaaaag gtccgaaact ttatatcttc gatttaaacg tcagccggca
     1441 gtttaaattt accgaaagat tccgtctgcg cccgtcgatc gaatttaata atattctaaa
     1501 tatgacggtc ttcagcttcg gttctaactt tattgatttc aaccttttaa atagcactaa
     1561 cccgacgacg gtcgccaacg cgcaggctgg atttttagcc cctacgcgaa cagttcgccc
     1621 gcgccagatt cggctcggcg tgagattcga tttctagacg gatttttaat cgaaatttta
     1681 cagcgagatt gttttgcgaa aaacagtctc gcttttttgt ttcaaaagcg aaaaaacttg
     1741 gtgacgagat ttgacgattt atgttagttt ataaatgttg aacgattttg ttcacaggcg
     1801 aaaaaatatg agcagtattt tagaaaaaga cgttcctttc tcagaaattt ccatcgaatc
     1861 ggttttgctc gacgttgatt tattcgttaa atatcgctcg gcggaaaaag ctttcagtat
     1921 cctgcgcgaa gcgattgagc gcagcccgcg ctcgattccc ctgcgcgaaa aaatgcgtga
     1981 tatctgcgtc gcgcacaaaa atctgaccga agccgcgcgg cattgtctgg cgctcgtctc
     2041 gctctacatc acgcgcgagg atttcgattt ggcatacgac cgcctgcagg aagcgaagct
     2101 gctcgacccg cgcatctcgg tcgcgcccgg actcgaagcg attcgccgcg cgcgccgtcc
     2161 cgaattcgcc gtccatcgcg aaaaatcttc ttacgaagtt cgctcggaca ccgcgtttgc
     2221 gggcgatttg gcgctggtca gtattttcga cgccgtgacg gtcgtggaaa attcccgtct
     2281 gaccggattg ctgattataa aatccgacag gcttttgggc agcgtttcct tcaacgaggg
     2341 aaaaatcgtg gacgcggaag ccaacggtca caacggcacg accgcgtttc gagatattat
     2401 cgaagtcgct agcggcgtat ttgaattttc cacttccgaa aacgagtttc ccgtcgtcat
     2461 acaagtttcc agcaacacga atttcctgct cgatattctc acgcagctcg acacggaaaa
     2521 agccgaaaag cagggaatga gaacgatgag cgccgacgat tttccggaat agctgccaaa
     2581 agtcagaaaa attagtcgcg aaatttcacg aaaaaatgag tgagttcttt aaatcaggat
     2641 tctaatctcc ctcaagttca ccgtaacctg ttttgagaaa atttgaagag gagccgtaat
     2701 catattctgc caggacatat tttgttgggt tatcttttcc taaaagataa ttggcatatc
     2761 ctcgataaaa caaatcttct gaagtcagcg gcgaatatcc aaaatttttc gagtctagaa
     2821 ttgtcccgca aacttcgcgc attttatttt gattgccgac agtttttaaa actcgcgcat
     2881 aatcaatcaa tagtcgccgc gttcttcttt cgggctgaca cagaaaattc tcatatatac
     2941 atatggcatt tgccggttgt ccgagccttg agtatttatc ggcaagatag tgataaagct
     3001 tcgttgtttt taatctttcg gtaatggaat ttaagaagct caaagcctcg gaatcttttc
     3061 cgcatttttc cagcgaaaga aaataataaa gcgcgccagc ctcgtcaaat tcctctaaag
     3121 tcagaaattg aaaaatttta acagcagttt catattctcc ttgccggtaa tggaaaagac
     3181 cctttatttt tattaagttt aatttattct caggggataa atccgacgag tcgatttgtt
     3241 caaataatgc ggctgcggcg ttagcgtcgc tgcaacaaga tgagtttatc agattagcta
     3301 agatgtattc atcattcggc atataatcca gtgcccgtct atacgcatcg actgattcgt
     3361 tataacgccc ggttaaaaaa tagacttgcc caagaccggc aaatgccaat cctaaaagcg
     3421 atttgtcata tatattttgc cactctgagg cttctgaaaa aatccgttcg gcggtttcca
     3481 gtcctttagc cctcagcttg ccctctttgg attgacttga aaatatctca aaaacgcctt
     3541 ccaaaatgaa aggataataa aacttaacgc ccggcgtgga gagagcgggt tctatcagaa
     3601 gcgaggaaac tatttttccg gctttctccc atttttcttc caaaaccatt tgtccgcact
     3661 tgttcatata tttagtgagt ttgttgaaat cggcaatagg caagttgaga tgttcggctt
     3721 cgcgaagaaa acgttcggcg aaaaaaacct tcgggtcttt tagaaaaggc ggaatgtatt
     3781 taacatttaa actattacat atccgacagg taatatcatc gagcttatct tcatttagcg
     3841 aggcagcctc aatccatttt atcccattca gttccgggta aatttcctgc caattttgcg
     3901 gcttgtttag caaaatcggc attaattttt cttccgaatg cctgccgctc tcaagcgcat
     3961 actgcacctc aaagcgtgga tatgcactca tctcctcatt cagactatga ggactgataa
     4021 ctgcgataaa atattcgcac gaatcaattt tcgcggcgag aaaagactta atgtctcgtc
     4081 ccgccgcgat gtcctgtcct aagtccgaat aatcccagca tttaatattc tgcagatcca
     4141 aagccgaaat caggaggcac acctctttct ggtctgcacg ggagaaactg ataaatacgc
     4201 aaggcaaatc ggacataacc tta
//
LOCUS       DASWNE010000212         2452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_322037, whole genome shotgun sequence.
ACCESSION   DASWNE010000212 DASWNE010000000
VERSION     DASWNE010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322037"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2338
                     /gene="carB"
                     /locus_tag="VGB68_04315"
     CDS             <1..2338
                     /gene="carB"
                     /locus_tag="VGB68_04315"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEY0048486.1"
                     /translation="EMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDEIPNDITKK
                     TPASFEPTIDYVVTKIPKWAFEKFPGAEDVLGTQMKSVGEVMAIGRTFKESLFKGLRS
                     LEAVKPLRLEGIPDHVLQRKLARPNSQRFSYITYALRNGYSIAEIHRVTKIDPWFLEQ
                     LQEVMQLQARIDGRSLNDVPADVLRTAKEFALSDRRISYLTSSTEAGVRERRKNLNIE
                     PVYKRVDTCGAEFESHTPYLYSTYEEESEAEPSERRKIMILGSGPNRIGQGIEFDYCC
                     CHASFALRDAGFETIMVNCNPETVSTDYDTSDRLYFEPLTFEDVMNIVETENPEGVIV
                     QFGGQTPLNLADRLHNAGVPIIGTSPDSIDLAEDRKRFGALLDELKIPSPENGTAISP
                     EEAKIIAGKIGYPIVVRPSYVLGGRAMAIVYDEQTLDEYMRTAVDASPEKPILIDKFL
                     ERAVEIDVDALADETTCIIAGIQEHIEEAGIHSGDSSSVLPAQKIAAEHLETIKHYTK
                     LLAKSLNVKGLMNIQFAVKDDRVYVLEVNPRASRTVPFVAKATGVPLAKIASLVMGGD
                     KTLEDFNLPEVLSVPKIFVKSPVFPFKKFAGVDPVLGPEMHSTGEVMGVGETFGEAYG
                     KAMEGAGLELPLEGKAFLSVNDNDKGQAVILAQRLNKLGFDLVATYGTTKRLQEVGLK
                     CETVFKVNEGRPNIADYIKQGEISLIINTPLGRTSHYDEQAIRKAALQFNVPCVTTMT
                     GAEALIEAIATKKSQEKVSVRSLQEIHSEKILKVKLFI"
BASE COUNT          629 a          660 c          635 g          528 t
ORIGIN      
        1 cgagatgaat ccgcgcgtct cgcgctcttc agcactagct tcgaaagcta cgggttttcc
       61 aatcgccaag atcgccgcga agcttgccgt cggatacaca ctcgacgaaa tccccaacga
      121 catcacgaaa aagacacccg catcctttga accgacgatc gattacgttg taacgaagat
      181 cccgaaatgg gcattcgaga agttccccgg agccgaagac gttctcggta cgcagatgaa
      241 atcggtcggc gaggtgatgg caatcggcag aacatttaaa gaatcgcttt tcaaaggtct
      301 tcgctctttg gaagcggtca agcctctgcg gctcgaaggc attcccgacc acgttttgca
      361 gcgcaagctg gctcgcccga actcgcagag attttcttac atcacatacg ccctgcgaaa
      421 cggttattcg attgcggaaa ttcaccgggt gacgaaaatc gacccgtggt ttctggagca
      481 gttgcaggaa gttatgcagc ttcaggcgcg aatcgacggg cgcagcctga acgacgttcc
      541 cgccgatgtt ttgcggacgg cgaaagaatt cgccttatcc gaccggcgca tttcctatct
      601 gaccagttcg accgaggcgg gcgtccgcga acgccgcaaa aatctgaaca tcgagcccgt
      661 ctataagcgc gtcgatacgt gcggcgcgga gtttgaatcg cacacgccgt atctctactc
      721 gacttacgag gaagaatcgg aagccgagcc gagcgagcgt cgaaaaatta tgattctcgg
      781 ttccggtccg aaccgcatcg ggcaaggaat cgagtttgat tactgctgct gtcacgccag
      841 tttcgcattg cgcgacgccg gtttcgagac gattatggtc aactgcaatc ccgaaaccgt
      901 atcgaccgat tacgacactt ccgaccgcct gtatttcgag cctctgacct ttgaagacgt
      961 gatgaacatc gtcgagaccg aaaatcccga aggcgtaatc gtccagttcg gcggtcagac
     1021 gccgcttaat ttagccgata gactgcacaa cgcgggcgtg ccgataatcg gcacttcgcc
     1081 cgattcgatt gatttggcgg aagaccggaa aagattcggc gctttgctcg acgaattgaa
     1141 aattccgtcg cccgaaaacg gcacggcgat ctcgccggaa gaagcgaaaa ttatcgccgg
     1201 taaaatcggt tatccgattg tcgtgcgccc gagctacgtg ctcggcggtc gcgcgatggc
     1261 gattgtctac gacgagcaga cgctggacga atatatgcgc acggcggtcg atgcttctcc
     1321 ggaaaagccg attttgattg acaaatttct cgagcgcgcc gtcgaaatcg acgtcgatgc
     1381 tttggcggat gaaacgacct gcatcatcgc cggaattcag gagcacatcg aggaagccgg
     1441 aattcattcg ggcgattcct cgtcggttct gcccgcgcag aaaatcgcgg cggaacacct
     1501 ggaaaccatc aaacattaca cgaagctgct ggcgaaatcc ttaaatgtaa aaggcttgat
     1561 gaacatccaa ttcgccgtca aagacgaccg cgtttacgtt ctcgaagtca acccgcgcgc
     1621 ttcccgaacc gttccgttcg tggcgaaagc gaccggcgtt ccgttggcga aaatcgcttc
     1681 gctggtgatg ggtggcgata aaactctgga agatttcaac ctgccggaag tcttgagcgt
     1741 cccgaaaatc ttcgtcaaat cgcccgtttt cccgttcaaa aaattcgccg gggtcgaccc
     1801 ggttctcgga cccgaaatgc actcgacggg cgaggtgatg ggcgtcggcg aaaccttcgg
     1861 cgaggcttac ggcaaagcga tggaaggcgc gggcttggaa ttgccgcttg aaggaaaagc
     1921 gtttctttcg gtcaacgata atgataaagg gcaagccgta attttagccc aaagattaaa
     1981 caaactcggc ttcgatttag ttgccactta tggcacgact aagcgccttc aagaagtcgg
     2041 cttaaaatgc gaaaccgtct tcaaagtcaa cgaaggtcgc ccgaacatcg ccgactacat
     2101 caaacaaggc gaaatttccc taatcatcaa cacgcctctc ggcagaacct cgcactacga
     2161 cgagcaggca atccgcaaag ccgctttgca attcaacgtt ccctgcgtca cgacaatgac
     2221 cggcgcggaa gctctcatcg aagccatcgc gacaaaaaaa tcgcaggaaa aagtgagcgt
     2281 ccgaagcttg caggaaattc actctgagaa aatattaaag gtgaaacttt ttatatgaaa
     2341 tgatatttgt gtgagaattt ccgtagttct atgtttgcag caaaatacga ccagtcaaac
     2401 tgtcgcctta atcccgtatt ttccaaaaca tctttacatg gcgtgtatat tc
//
LOCUS       DASWNE010000213         2287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_112208, whole genome shotgun sequence.
ACCESSION   DASWNE010000213 DASWNE010000000
VERSION     DASWNE010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112208"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VGB68_04320"
     CDS             <1..373
                     /locus_tag="VGB68_04320"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEY0048487.1"
                     /translation="PLKDYGLNLEPIGIEEIAAATVDDYCGEKEIEQIDLLKIDTEGA
                     EFQVMKGARKMLKSGRVKCLTFEFGQTTFDMGNRPEEIEDFLGEMNYGVRNIVKGNPV
                     FPGRKSVETAKFSMHVATPRA"
     gene            422..1732
                     /locus_tag="VGB68_04325"
     CDS             422..1732
                     /locus_tag="VGB68_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048488.1"
                     /translation="MISLKPTPETLHECPRCGLKLAPQGWLITGMRNLAELRCLRCGD
                     EFYGDLPSGQALYTPILLNKSSGAVYDDDNAGWFAGWLRDSYARRTIEPLRFEIKRFS
                     KIEKKVVLLNCLDTLYGHSLLKLLNAQYYVNRSDVSLIVLLPPFLEWMLPSGAAQAWI
                     ADLPLRRGTEWNEWLAREIEKLLEPFQEVFLSVGFSHPHADDFEIERFTRIKPFPLEN
                     WAESVGKPRVTFIWRDDRLWETKAPDESQNRFGKIKRRFGKPAARTERQLQKVIRFAE
                     CLRRQAPALDFAVAGLGEASGLPDWISDLRLTNLDAAAERAWCERYARSHIVVGVHGS
                     NMLLPSAHAGAVIELIGEDRRGNFLQDILFRPSDFREMFFRYRFVPHSTAPEELARLA
                     ASVLRYEDFRRLMSPEFCRHRENYDFRQFQSPRGGGQIDGERRL"
     gene            1732..>2287
                     /locus_tag="VGB68_04330"
     CDS             1732..>2287
                     /locus_tag="VGB68_04330"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048489.1"
                     /translation="MCGISGIVTREKLSAGLSAAVSRMSAAQIHRGPDGAGEFSAAHV
                     NLAMRRLSIIDLEGGAQPLYNEDASLALIINGEIYNYRELRDRLKKSGHTFRTETDGE
                     VILHLYEENDTDFVTHLRGMFAFALWDCKRRRLVLARDRMGEKPLYLFERDGALVFAS
                     ELKGLLHSGLVPFELNPRAVNLYFH"
BASE COUNT          507 a          573 c          671 g          536 t
ORIGIN      
        1 accgctaaag gattacgggc ttaatcttga gccgatcgga attgaggaaa ttgcggcggc
       61 gaccgtcgac gattattgcg gcgagaaaga aatcgagcaa atcgatttgc tcaagattga
      121 tacggaaggc gccgaatttc aggtgatgaa aggcgcgcgc aagatgctga agtccgggcg
      181 cgtaaaatgt ctgactttcg agttcgggca gaccactttc gatatgggaa atcgcccgga
      241 agagatagag gattttctcg gggaaatgaa ttacggcgtt cgcaatatcg ttaaaggaaa
      301 tccggttttt cccggtcgga aaagcgtcga aacggctaaa ttttcgatgc acgtggcgac
      361 gccgcgagct taaaaagccg cgttaaaaat tgttcggcta ttctctgcat ttgcattata
      421 aatgattagt ttaaaaccta ctcccgaaac cttgcacgaa tgcccgcgct gcggtttaaa
      481 gctcgcgccg caaggctggc tgataaccgg aatgcgtaat ctcgccgagc tgcgatgttt
      541 gcggtgcggc gacgaatttt acggcgattt gccttcggga caggcattgt acacgccgat
      601 tttgttaaat aaatcgagcg gcgcggtcta tgacgacgac aacgccggct ggtttgccgg
      661 atggctgcgg gattcttacg cccgacgaac aattgagccg cttcgctttg agataaaaag
      721 gttttcaaaa atcgaaaaaa aagtggtctt gctcaattgt ctggacacgc tttacggtca
      781 tagtttattg aaactgctca acgcgcagta ttacgtaaat cgctcggacg tcagcttaat
      841 cgtcctgctg ccgccttttc tcgaatggat gctgccgagc ggcgcggcgc aggcgtggat
      901 tgcggattta ccgctgcggc gcggaaccga gtggaacgaa tggctggcgc gcgaaatcga
      961 aaaacttctc gaaccgtttc aggaggtttt tttgagcgtc ggtttttcgc acccgcacgc
     1021 cgacgatttc gagattgaac ggtttacgag aataaaacct tttccgctgg aaaattgggc
     1081 tgagtcggtc ggcaaaccgc gcgtaacttt tatctggaga gacgaccgtt tgtgggaaac
     1141 gaaagcgccg gatgaatctc aaaatcgttt cgggaaaata aaacggcgat tcggcaaacc
     1201 cgcggcgcga accgaacgcc agctgcaaaa agtcatacga tttgcggaat gcctgcgcag
     1261 gcaggctccg gcgctggatt tcgccgtcgc cggactgggc gaagcgagcg gattgcccga
     1321 ttggatttcc gatttgagat tgactaattt agacgccgcc gcggaacgcg cctggtgcga
     1381 gcgttatgcc cgaagccaca tcgtcgtcgg cgttcacggc tcgaatatgc ttctgccgtc
     1441 agcccacgcg ggcgcggtta tcgagcttat cggcgaagac cgccggggaa atttcctgca
     1501 ggatattttg tttcgcccgt cggattttcg cgagatgttt tttcgttacc gcttcgtgcc
     1561 gcattcgacc gcgccggaag aattagcccg tctggcggct tcggttctgc gatacgaaga
     1621 cttccggcgg ctgatgagtc cggagttttg ccgtcaccgg gaaaattatg atttcagaca
     1681 atttcagtcg ccgcgcggcg gcgggcaaat tgacggggag aggcggcttt aatgtgcggc
     1741 attagcggaa tcgtaacgcg ggaaaaactg tccgccggtt tgtcggcggc ggtttcgcgg
     1801 atgagcgcgg cgcagattca tcgcggtccg gacggcgcgg gcgagttttc cgccgcgcac
     1861 gtcaatctgg cgatgcgccg cctttccatc atcgacctcg aaggcggcgc tcagccgctc
     1921 tataacgaag acgcgtcgct cgctctgatt atcaacggcg aaatttataa ttaccgcgag
     1981 ctgcgcgacc gtctaaaaaa atcgggacat acgtttcgca ccgagacgga cggcgaagtg
     2041 attctgcatc tctacgagga gaatgatacg gatttcgtca cgcacctgcg cggaatgttc
     2101 gctttcgctc tgtgggactg caagcggcgg cgtcttgtcc tcgcccgcga ccggatgggc
     2161 gaaaaacctc tgtatctttt cgagcgcgac ggcgcgcttg ttttcgcctc ggaattaaag
     2221 ggattgctgc attcggggct ggttccgttc gagctgaatc cgcgtgccgt caatctttat
     2281 tttcatt
//
LOCUS       DASWNE010000214         3602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_676563, whole genome shotgun sequence.
ACCESSION   DASWNE010000214 DASWNE010000000
VERSION     DASWNE010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676563"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..962
                     /locus_tag="VGB68_04335"
     CDS             60..962
                     /locus_tag="VGB68_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048490.1"
                     /translation="MSEHIKESVRALLDGIVDYAGLFPPSGLSMPEAVINYATYKNSN
                     YNWMLGRFIVPVGRLAEFTGSAKDFFTRSGQDAWKLSVLASEDIYETVRLVEDFNAEH
                     APFAQCDTLEIKAASASFIESCAAAVPPQFTNYFEIPIGEQLAELVVTLAIGGQRAKL
                     RTGGTTPEAFPRPDEITRFIRTCSAANVPFKATAGLHHPVRCQKSLTYEKDSPVGMMY
                     GFLNVFLAAAFARQGFKPSLLNELLEDEWTESFVFDANGVWWRQEHFLSSAYLKLLRE
                     RGAISFGSCSFEEPIADLQDLEIL"
     gene            1154..2494
                     /gene="fahA"
                     /locus_tag="VGB68_04340"
     CDS             1154..2494
                     /gene="fahA"
                     /locus_tag="VGB68_04340"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HEY0048491.1"
                     /translation="MTFEINETHDLNLKSWVESANAPDTDFPIQNLPYCFFYERDNLK
                     HERLGFAIGDKVLDFDSCWEQGLFGNKESEEYKSLEDFTYDFSLNRGVENLHPKTISF
                     IRRRLIEALSENASEHDRKIVEKNLLSIREVEFLVPFQIGDYTDFYCSIFHAANVGSM
                     FRPDNPLLPNYKWLPIGYHGRASSIVISGTEIKRPKGQNRADAEQPPVFIPCKNLDYE
                     MEVGFFVGQGNDLGEPVSIQDAEKHIFGLCLVNDWSARDIQAWEYQPLGPFLAKNFAT
                     SVSPFVVTMEALAPFRTKAFERAEGDPPPLDYLYSEANETGGGFDVELEVYIQTRKMR
                     DESTEPFLLSRSNMKDLYWTIRQMLTHHASNGCNLQTGDLMATGTVSGKSKKERGCQL
                     ELTWRGKEPVELPNGEIRRFLEDGDEIIMKGFCRREGFRRIGFGECRGIILPAD"
     gene            2628..>3602
                     /gene="purF"
                     /locus_tag="VGB68_04345"
     CDS             2628..>3602
                     /gene="purF"
                     /locus_tag="VGB68_04345"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEY0048492.1"
                     /translation="MDFSLDKFHEECGIFGIFGHDEASTLTQLGLFALQHRGQEACGI
                     VSSDGEELHQFRSQGLVADVLTEEVLKKLEGTSAIGHTRYSTAGKNTIKEVQPFAVTC
                     QHGQLAVCHNGNLPFANRRRAELERAGAIFSSTSDTETILHSIARTSAKDVIEAVSTV
                     LRETEGAFSLLFLTPTALVAVRDPRGFRPLVLGKLKDAWCVASETCAFDLIDAQYVRE
                     IKAGEMLVINENGLHSYFPFEEKPLSVCAFEHVYFSRPDSTVYSRSVNESRHKMGKQL
                     AIEHPADADLVVPVPDSGVAAAIGYARQSGINYRQAIIRNHYVGRTFIE"
BASE COUNT          836 a          909 c          969 g          888 t
ORIGIN      
        1 gtgacgttgt ttttcccgcc gatgttttac cgtttcaaat gggagaaact gtcgaataaa
       61 tgagcgaaca catcaaagaa tccgtccgcg ctttgctcga cggaattgtc gattacgccg
      121 gactttttcc gccttccggg ctttctatgc cggaagcggt catcaattac gcgacctata
      181 aaaacagcaa ttacaactgg atgctcggac gttttatcgt gccggtcggt cggctcgcgg
      241 aatttaccgg aagcgcgaaa gattttttta cgcgcagcgg gcaggacgcg tggaaactga
      301 gcgttctggc aagcgaagat atttacgaaa ccgttcgcct ggtcgaggat tttaacgccg
      361 agcacgcgcc ctttgcgcaa tgcgatacgc ttgagataaa agccgcttcc gcttcgttta
      421 tcgaaagctg cgccgcagcc gtgccgccgc agtttaccaa ttattttgaa attccgattg
      481 gcgaacagct tgccgaattg gtcgtgacgc tggcaatcgg cggacagcgc gccaagcttc
      541 gcacgggcgg cacgacgccg gaagcctttc cgcgaccgga tgaaataacg cgttttatac
      601 gcacctgctc ggcggcaaac gtcccgttta aagccaccgc cggactgcat catccggtgc
      661 gctgtcaaaa atctctgacc tacgaaaagg attcgccggt cggaatgatg tatggctttc
      721 tgaacgtctt tctggcggcg gcttttgcgc gtcagggctt taaaccgagc ttgctcaacg
      781 agttgctgga agacgaatgg acggaaagct tcgtttttga cgccaacggc gtctggtggc
      841 ggcaggagca ttttctgagc agcgcgtatc tgaaactgct gcgcgagcgc ggcgcgattt
      901 ccttcggctc ctgctcgttc gaagagccga ttgccgattt gcaggatttg gagattttgt
      961 aaagaaatta acgaaacttc gaaacaaatg cgggcgcgcg ccgccgcgcc tgttcaatcg
     1021 agcgcggcga ataaacgttt taaatatttc agtattgaat ttaatcaaaa ttgattagcg
     1081 ttccgccgag cgctccgggc agtcgccgcc gcggcgtgcc cgtactaaat tcggcggcga
     1141 ttgtaatttt cttatgacat ttgagataaa cgaaacacac gacctgaatt taaaaagctg
     1201 ggttgaatca gccaacgcgc ccgatacgga ttttccgatt caaaatctgc cttactgctt
     1261 tttttatgaa agagacaatt taaaacacga gcgactgggc tttgccatcg gcgataaggt
     1321 gctggatttc gattcgtgct gggaacaggg cttgttcgga aataaggaat ccgaagaata
     1381 taaatcgctc gaagatttta cctatgattt ttctttaaat cgcggcgttg aaaatctgca
     1441 tccgaagacg atttccttta ttcgtcgtcg tttgattgaa gctctctcgg aaaacgcgtc
     1501 tgagcacgac cgcaaaatcg tcgagaaaaa cttgctttca atacgcgaag tcgaatttct
     1561 cgtccctttc caaatcggag attacaccga tttttattgc tcgatttttc acgctgccaa
     1621 cgtcggctcg atgtttcgtc ccgataatcc gcttctgccg aattacaaat ggcttcccat
     1681 cggttatcac gggcgcgctt cttcgattgt aatttcggga acggaaatca aacgtccgaa
     1741 aggtcaaaac cgtgcggatg ccgaacagcc gccggttttt attccctgca aaaatctcga
     1801 ttacgagatg gaagtcggat tttttgtcgg gcagggaaac gatttgggcg agccggtttc
     1861 gattcaggac gcggaaaaac atattttcgg tttgtgtctg gtcaacgatt ggtcggcgcg
     1921 cgacatacag gcttgggaat atcagccttt gggtcctttt ctcgccaaaa attttgcgac
     1981 cagcgtctcg cctttcgtcg tcacgatgga agcgctcgcg ccgtttcgca cgaaagcctt
     2041 tgaacgggcg gaaggcgacc cgccgccgct cgattattta tattccgaag cgaacgagac
     2101 gggcggcggc tttgacgtcg agctcgaagt ttatattcaa acgcggaaaa tgcgcgacga
     2161 atcgacggag ccgtttctgc tttcgcgctc gaatatgaaa gatttatatt ggacgatcag
     2221 gcagatgctg acgcaccacg cctcaaacgg ctgcaacctg caaaccggcg atttgatggc
     2281 aacggggacg gtttccggca aatcaaaaaa ggagcgcggc tgccagctgg aactcacctg
     2341 gcgcggcaaa gaacctgtcg aactgccgaa cggcgaaatt cgtcgctttc tcgaagacgg
     2401 cgacgagata attatgaaag gcttctgccg gcgcgaaggc tttcgccgca tcggcttcgg
     2461 cgaatgtcgc ggcataattt taccagccga ttaatcaaaa aagattttaa acaggatgag
     2521 caggataaac cggataaact cgatttgaat gatttctttt tatcctgttc attgagttgc
     2581 gaagcctgct ctatcctgtt taattttcct gtggcaaaat ataagtaatg gatttttccc
     2641 tcgataagtt tcacgaagaa tgcggcattt tcggcatttt cgggcacgac gaagcctcga
     2701 ctttgacgca gctcggactt ttcgctttgc agcatcgcgg acaggaagcc tgcggtatcg
     2761 tatcgagcga cggcgaggag ctgcaccaat ttcgctcgca ggggctggtc gccgatgttt
     2821 tgaccgaaga ggttttgaaa aagctcgaag gcacgagcgc catcgggcac acgcgttatt
     2881 cgaccgccgg aaaaaacacg attaaggaag tgcagccgtt tgccgtcacc tgccagcacg
     2941 ggcagctcgc cgtctgtcat aacggaaatc tgcctttcgc caatcgaagg cgcgccgagc
     3001 tggaacgagc gggcgcgatt ttttcgtcca cctccgacac ggaaacgatt ctgcattcga
     3061 ttgcgcgcac ctcggcaaaa gacgttatcg aagcggtttc gacggttttg cgcgaaaccg
     3121 aaggcgcgtt ttcgcttttg tttttgacgc cgacggcttt ggttgccgtg cgcgacccgc
     3181 gcggatttcg cccgctcgtt ttgggaaaat tgaaagacgc ctggtgcgtc gcgtcggaaa
     3241 cctgcgcgtt tgatttgatt gacgcccaat acgtccgcga aatcaaagcg ggcgagatgc
     3301 ttgtaattaa tgagaacgga ctgcattcgt atttcccgtt tgaggaaaag ccgctttccg
     3361 tctgcgcgtt tgaacacgtc tatttttcgc gtcccgattc gaccgtttac agtcgttcgg
     3421 tcaacgaatc gcgtcataaa atgggcaagc aactggcaat cgagcatccg gcggacgcgg
     3481 atttggtcgt gccggttccc gattcgggcg tggcggcggc aatcggctac gcgcggcagt
     3541 cgggcatcaa ttaccggcag gcgattatcc gcaatcacta cgtcgggcgc acgtttatcg
     3601 ag
//
LOCUS       DASWNE010000215         4315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_77393, whole genome shotgun sequence.
ACCESSION   DASWNE010000215 DASWNE010000000
VERSION     DASWNE010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77393"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="VGB68_04350"
     CDS             complement(<1..970)
                     /locus_tag="VGB68_04350"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0048493.1"
                     /translation="MSFILNLTFREIRSSWRRLLFFFLCIAIGVGSVVALRSLIQNMS
                     RAVAGDARALLTADFEISSTNPFTPSELSAIESVIAKSSVIEARSETVTTAAMTRTAE
                     NFQFVELKGIEPPFPLVGDFVLAGDKAFDFSLLENKGAVVQATLLDKLNLKIGDKIRI
                     GESDFEIRAAFDEEPGGTGGFRLGPRIFIERKLFDEAGLTQSRIRRRILYRTSDDPTP
                     LVSELRQALKGTVLTVNSYKEAQENLGEQFERTENYLALTGLLILVLGGIGVWNVARV
                     FVEQKRKTVAVLKCLGAGGWRIITVYLLQILTLGALGSVFGVVLAQA"
     gene            complement(1046..1759)
                     /locus_tag="VGB68_04355"
     CDS             complement(1046..1759)
                     /locus_tag="VGB68_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0048494.1"
                     /translation="MIQLKNITKIVRSGAEDLTILDDVSLEIPDGEFIAVTGASGSGK
                     STLLGLIAGLDAPSSGEILIDDELINAMSEDALARLRSEKIGFIFQSFHLIPSLTAFE
                     NVLVPMEISGFKNAKETARKLLEDVELTNRGHHYPSELSGGEQQRIAIARAFANQPKI
                     LLADEPTGNLDSRSGSHIFDLMTRLHDNNQTTLVLVTHDRELANKAHRQIRLRDGKVI
                     SDEINRAEAEKKIFRGEQA"
     gene            1930..2628
                     /locus_tag="VGB68_04360"
     CDS             1930..2628
                     /locus_tag="VGB68_04360"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEY0048495.1"
                     /translation="MFSVRKNTYALIGFLVLSLFVAACGTEASRLGNQKPKDAAKLPP
                     TSSNKPKIVAFGDSLTAGFGLSEKESYPFLLQQRLNAEGYNYEVVNAGVSGDTSQGGL
                     ERIDWSLEQENVQILVLELGANDLLRGLSPAKMKKNLGEIIKKAQAKKVQVLLCGMLA
                     PPTMGARYQQEFIKAFPDLADEYKVEFLPFILEGIALDKKLNQADGIHPNAEGAKILA
                     ENVYKGLKPLLRKN"
     gene            complement(2709..3575)
                     /locus_tag="VGB68_04365"
     CDS             complement(2709..3575)
                     /locus_tag="VGB68_04365"
                     /inference="COORDINATES: protein motif:HMM:NF017672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006672 - ceramide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ceramidase domain-containing protein"
                     /protein_id="HEY0048496.1"
                     /translation="MKKYKGFFVWILGTFFILTAFLIFRDIGWGGAPDECIFEKPDGC
                     YCEAFKKDDVVKDKPGVRQPVNTWSNLYAIITSFIVAWGVYSDRRKYSNDYSDADAAP
                     NIIGSSSWKADLYIFAVLFLGLGSMWFHASLKEWGGLLDGVSMYVFASYLVFYSARRF
                     FDSDRVFWLGYLCSILFFTLMSNLGVDSVINIGILVGAYATLEIYIWWRTKVMQGKTK
                     TVLLWIFAFVSIITATVFRALSETGKDLCYPDSWFQPHGLIWHPLAGVMAVLLYFYWR
                     DADDRNELGTKI"
     gene            complement(3930..4163)
                     /locus_tag="VGB68_04370"
     CDS             complement(3930..4163)
                     /locus_tag="VGB68_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048497.1"
                     /translation="MDGKQTLEVQKQAEADAARDGAAGSEWDFHDMILGAKSQVKAQF
                     GEDSDEYASLGMKKKSEYKRGRRKATPTEAATS"
BASE COUNT         1133 a         1120 c         1056 g         1006 t
ORIGIN      
        1 ccgcctgcgc cagaacgacg ccgaagacgc tgcccagcgc gccgagcgtg agaatctgca
       61 aaagataaac cgtgatgatt cgccagccgc ccgcgccgag gcattttaaa acggcgacgg
      121 ttttgcgttt ctgctcgacg aaaacgcgcg cgacgttcca cacgccgatg ccgcctaaaa
      181 ccagaatcag caagccggtc agcgccagat aattttccgt gcgctcgaat tgctcgccca
      241 gattttcctg cgcttccttg taggaattga ccgtcagaac cgtgcctttc agcgcctgcc
      301 gcaattcgga aaccagcggc gtcgggtcgt ccgacgtgcg atacaaaatc cgccgccgga
      361 tgcgcgattg agtcaaaccc gcttcgtcaa ataattttct ctcgataaaa atgcgcggtc
      421 ccagacgaaa accgcccgtt ccgcccggtt cttcgtcaaa cgcggcgcga atctcgaaat
      481 cgctttcgcc gatgcggatt ttatcgccga ttttcaaatt cagcttgtct aaaagcgtcg
      541 cctgcacgac cgcgcctttg ttttccagaa gcgaaaaatc gaaagcctta tcgcccgcca
      601 gcacgaaatc gccgaccagc ggaaaaggcg gctcgatgcc tttcagctcg acaaactgaa
      661 aattttcggc ggtgcgcgtc atcgcggcgg tcgtgaccgt ttcgctgcgc gcctcgataa
      721 cgcttgattt agcgataacg ctttcaatcg ccgaaagctc gctcggcgta aaaggattag
      781 tcgagctgat ttcaaaatcc gccgtcagaa gcgcgcgcgc atcgcccgcc acggcgcggc
      841 tcatattttg aatcagcgag cgcagcgcga cgaccgagcc gacgccgatg gcgatgcaca
      901 aaaagaagaa caaaagcctg cgccacgacg agcggatttc gcgaaaggtt aaatttaaaa
      961 tgaaagacat atttttcaac accgaataca aagaggaaat tttcagggac gaaggagata
     1021 aaaaagaaag ctgtttgctt gtcttttatg cctgttcgcc cctgaaaatt tttttctccg
     1081 cctctgcgcg gttaatttca tcgctgataa ccttcccgtc gcgcagacga atttgccggt
     1141 gcgctttatt tgccagttcc cggtcgtgcg taactaaaac cagagtcgtc tgattattgt
     1201 cgtgcaatct cgtcatcaaa tcgaaaatgt gcgagccgct gcgcgagtcg agattgccgg
     1261 tcggctcgtc cgccagaaga attttcggct ggttggcgaa agcgcgggcg atggcgatgc
     1321 gctgctgttc gccgcccgat aattccgaag ggtaatgatg cccgcggttc gtcagttcga
     1381 cgtcttcgag cagtttgcgg gcggtttctt tagcattttt aaaaccggaa atctccatcg
     1441 gcacgagcac gttttcgaat gccgtcaacg acggaatcag gtgaaacgat tgaaagataa
     1501 aaccgatttt ttcgcttctg agccgcgcca gagcatcttc gctcatcgcg ttaatcagct
     1561 cgtcgtcaat cagaatctcg cccgaactcg gcgcgtccag cccggcaatc agaccgagca
     1621 gagtcgattt gccgctgccc gacgcgccgg tgacggcaat aaattcgccg tccggaattt
     1681 ccagcgaaac gtcgtccaga atggttaaat cttccgcgcc cgagcggacg attttggtaa
     1741 tatttttaag ttgaatcatt gcgttattaa gattcaggat tcacgattcg gaatttaaga
     1801 ttcaggattt aagatttaag attagaatcc tgaatcctga atcctgaatt ttaaatcctg
     1861 aatcctaaca tttagactaa acaaaatagg cgaaactcgc atctaaaatt tggttttgaa
     1921 gtaaatatta tgttctcagt cagaaaaaat acatacgcat taatcgggtt tctcgtttta
     1981 tctttattcg tggcggcttg cggaacggag gcgagcagat taggaaatca aaagccgaaa
     2041 gacgcggcga agctgccgcc gaccagctcg aacaaaccga aaatcgtcgc gttcggcgac
     2101 agtttgaccg ccggattcgg cttgagcgaa aaggaatcgt acccgtttct gctccagcag
     2161 aggttaaacg ccgaaggcta taattacgaa gtcgtcaacg cgggcgtctc cggcgacacg
     2221 agccagggcg ggctggaaag aatcgactgg tcgctggaac aggagaacgt gcagattctg
     2281 gttctggagc tcggcgccaa cgaccttctg cgcggtcttt cgccggcgaa aatgaaaaag
     2341 aatctcggcg agattatcaa aaaagcgcag gcgaaaaaag ttcaggttct tttatgcgga
     2401 atgctcgcgc cgccgacgat gggcgcgaga tatcagcagg aattcatcaa ggcttttccc
     2461 gatttggcgg acgaatacaa ggtcgagttt ctgccgttta ttctggaagg catcgcgctg
     2521 gataaaaagc tcaatcaggc tgacggcatt catccgaacg ccgagggcgc aaagattctg
     2581 gcggaaaacg tttacaaagg cttaaagccg ctgctgcgga aaaactgatg taaactcggc
     2641 gggaaaagaa aaaactgata actgagaact tgttttttat tagccggttc ccggttatca
     2701 gttgatttct aaatcttcgt gccgagctcg tttcggtcgt cggcgtcgcg ccagtagaaa
     2761 tagagcagca cagccattac gcccgccagc ggatgccaga tcagcccgtg cggctgaaac
     2821 cagctgtccg ggtagcacaa atctttgccg gtctcggaca aggcgcgaaa aacggtcgcc
     2881 gtgataatcg agacgaaagc gaatatccag agcagaacgg ttttggtttt gccctgcatt
     2941 acttttgttc gccaccagat atatatttca agcgtcgcgt aagcgccgac cagaatcccg
     3001 atgttgataa cggaatcgac tccgagattg ctcatcaaag taaaaaacag gatgctgcaa
     3061 aggtatccga gccagaaaac gcggtcggaa tcgaaaaacc ggcgcgccga ataaaagacc
     3121 agatacgacg cgaagacata catcgacacg ccgtcgagca aaccgcccca ttccttcagc
     3181 gaagcgtgaa accacatact gccgagtccg agaaacaaga ccgcaaagat atataaatcc
     3241 gctttccacg agcttgagcc gataatgttc ggcgcggcgt ctgcgtccga ataatcgtta
     3301 ctgtatttcc tgcggtctga ataaacgccc caggcgacaa taaacgacgt aataatggcg
     3361 taaagattag accacgtgtt cacgggctgc cgcacgcccg gcttatcctt gacgacgtcg
     3421 tcctttttaa acgcttcgca gtaacagccg tcgggctttt caaaaatgca ttcgtccggc
     3481 gcgcctcccc agccgatgtc gcgaaaaatt aaaaaagccg tgagaataaa aaacgtgccc
     3541 agaatccaga caaagaatcc tttgtatttt ttcattatca atcctcctgt ttgtcggcga
     3601 acagcttata taccgaaatt gcagtgtaaa acccgaatgg cttgctttag attacgcgtg
     3661 cgaggcgcgc cagccccgca agctttgcgt aaaaggcgaa agacaaggaa tttgcctttt
     3721 gaatgccaat caatatagga aaaaataaaa ataatgtaaa gaaaaactgc cgcgcctgta
     3781 agttttaaat tgattaatat cgaagttttt tgaaacgccg agccgtgccg cctaatcaca
     3841 tttaataatt caaagagcaa tcaggacaca aaaaagccgc ctgcccgaac aaagcagacg
     3901 gctttttctt ttcaaccgga aatctctttc taagaggtcg ccgcttcggt cggcgtcgct
     3961 ttgcgccgtc cccttttata ctcgctcttt ttcttcatac cgagcgatgc gtattcgtcc
     4021 gagtcttcgc cgaactgcgc tttgacctgg cttttcgcgc cgagaatcat atcgtgaaaa
     4081 tcccattccg aaccggctgc gccgtcgcgc gccgcgtccg cctcggcttg cttctgcact
     4141 tcgagcgtct gtttgccgtc catcgatgtt ttccagatag taataaatga gcatgaagaa
     4201 aaataaacgt tttactaaat cgatattctt aacctaatgt ttatacatat tttcgatacg
     4261 taatagtttt agtcatcttc cagcttttgg attattatcg ccgcaaaaac ctgtg
//
LOCUS       DASWNE010000216         1994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_218096, whole genome shotgun sequence.
ACCESSION   DASWNE010000216 DASWNE010000000
VERSION     DASWNE010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218096"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VGB68_04375"
     CDS             <1..249
                     /locus_tag="VGB68_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048498.1"
                     /translation="LEKIDFGKHALVGIEINSGYCRIPLGLRYQAYKDADKKQYLLGI
                     SYIEPRGAVCRALSQYDLWLLVPKLPAGYEVKFDVKAK"
     gene            388..1182
                     /locus_tag="VGB68_04380"
     CDS             388..1182
                     /locus_tag="VGB68_04380"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEY0048499.1"
                     /translation="MPFTSDPFANAFIATAIAGFGATTLGAIPAFFLRRLNEKLNNNL
                     LSFAAGVMLAATIFSLLLPSIANSKAQGATTTSAVLQTIFFLFVGGFVLWFINALVPH
                     EHFGKGHDGVIDSGKIRKIWLFVIAIAIHNFPEGLSVGVANGTSEIGVGLGATLGIGL
                     QNIPEGLSVAVALHSQGYSKKYAFGVSALTGLVEVFGGISGASILLISSHVLPWALAF
                     AGGAMLFIVSDEIIPETHRHGFENGATFSLFVGFGLMMFLDAVFAQ"
     gene            1415..1768
                     /locus_tag="VGB68_04385"
     CDS             1415..1768
                     /locus_tag="VGB68_04385"
                     /inference="COORDINATES: protein motif:HMM:NF025183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HEY0048500.1"
                     /translation="MKDVQEILVNLQASEFRRSVRLHRKEKEYLNRKGLETILEHAAD
                     FVEKRLAPAEPANDGKQTPWRNHPVFIAQHATATCCRGCLEKWHDIPKGQPLGDAEKQ
                     HVVEIIKRWLCRNIE"
BASE COUNT          494 a          485 c          458 g          557 t
ORIGIN      
        1 ctcgaaaaaa ttgatttcgg caaacacgct ctggtcggca tcgagataaa ttccggctat
       61 tgccgcattc cgctcggtct tcgatatcaa gcctacaaag acgccgacaa aaaacaatat
      121 cttctcggca tcagctacat cgaaccgcgc ggtgcggtct gccgcgcctt aagccaatac
      181 gatttgtggc tgctcgtgcc gaagcttccc gccggttacg aagttaaatt cgacgttaaa
      241 gccaagtgat taatgcaaaa tacgaagcgc cgggcgcgaa attaaaagaa cagattcaat
      301 cctgaaacct aaattccaat tccggaattc taaattccga atccttgcat tttgcatttt
      361 gcgttttgca ttttgcatta tatagagatg ccttttacgt cagacccatt cgccaatgcc
      421 ttcatcgcca ccgctattgc cggtttcggc gcgacgaccc tcggcgcgat tccggcgttt
      481 ttcctgaggc ggctcaatga aaaattaaac aacaacctgc tcagctttgc ggcgggcgtg
      541 atgctggcgg cgacaatttt ttcgcttttg ctgccgtcaa tcgccaactc gaaagcgcag
      601 ggcgcgacga ccacaagcgc ggttttgcag accatctttt tcctttttgt cggcggcttc
      661 gtgctttggt ttatcaacgc gctcgtcccg cacgaacatt tcggcaaagg tcacgacggc
      721 gtgattgatt cgggcaaaat cagaaaaatc tggcttttcg tcatcgccat cgcgattcat
      781 aattttcccg aaggtctttc ggtcggcgta gcaaatggaa cgagcgagat tggcgtcggt
      841 ttgggcgcaa ctctgggtat cggtctgcaa aacattccgg aaggcttatc ggtcgccgtc
      901 gcgcttcatt cgcagggcta ttccaaaaaa tacgctttcg gcgtttcggc gctgaccggc
      961 ttggtcgagg ttttcggcgg catttccggc gcgtcgattc tgctgatttc gtcgcacgtt
     1021 ctgccgtggg cgctggcatt tgcgggcggc gcgatgctct ttatcgtcag cgacgagatt
     1081 attcccgaaa cgcatcgtca cggctttgaa aacggcgcga ccttttcgct gtttgtcggc
     1141 ttcggtctga tgatgtttct cgacgcggtt ttcgcgcagt aaaattgcgg cgaaatgtga
     1201 aaattctctt tcgggttaca tcaatcaggc tttaaaattc ttttatgctt tgcacaaaaa
     1261 taaaggaaat cgagaaaaat tccgacggct aattgtaaat tctcaattat taattctcaa
     1321 ttacttcaca aaagataccg aacatcaatt tagaatttac aatttagaat ttacaatttt
     1381 ccctttcttt atttttgtgc aaagccattc ttttatgaaa gatgtgcagg aaattctggt
     1441 taatctgcag gcttcagagt ttcgccgcag cgtccggctt caccggaagg agaaggaata
     1501 tctcaaccgg aaaggtctgg aaacaattct cgaacacgcc gccgactttg tcgaaaagcg
     1561 gcttgcgccc gccgaaccag ccaatgacgg aaaacaaacg ccctggcgca atcatccggt
     1621 ttttatcgcc cagcacgcga cggcaacctg ttgtcgcggc tgcctcgaaa aatggcacga
     1681 cattccgaaa ggacagccgc tcggcgacgc cgaaaagcaa cacgtcgtcg aaattatcaa
     1741 aagatggctt tgtcggaata tcgagtaaaa tccggcgtta ttttcgtata attgcggagc
     1801 acgctcgttt ccggtcttta aatttgcgaa agataatcag gtttaaattt cggatattat
     1861 ccgcttttga gactttgtat ttaagtgtat tataaatctg catcagctca ataattttta
     1921 ttgattcgga ttcgattaaa acaatctgca aattggagtt ttccttaaat gaaattttta
     1981 actattttct ttgc
//
LOCUS       DASWNE010000217         4599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_289172, whole genome shotgun sequence.
ACCESSION   DASWNE010000217 DASWNE010000000
VERSION     DASWNE010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4599
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289172"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(241..495)
                     /gene="rpsP"
                     /locus_tag="VGB68_04390"
     CDS             complement(241..495)
                     /gene="rpsP"
                     /locus_tag="VGB68_04390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEY0048501.1"
                     /translation="MLAISLMRMGAKGKPFYRLVVKEKRSKRDGKYIENVGTYNPMLN
                     PAEVNLKHDRIQYWISVGAQPTETVASLIKNNPAAAATEE"
     gene            complement(569..2071)
                     /gene="ffh"
                     /locus_tag="VGB68_04395"
     CDS             complement(569..2071)
                     /gene="ffh"
                     /locus_tag="VGB68_04395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEY0048502.1"
                     /translation="MFESLSDKLKKTLKNLRGEGKLTPEHVDVALREIRMALLEADVN
                     YKVAKDFVESVRSKAEGQEVWQDLKPHEQVVKIVYDELVELMGGTSSRLVFTRAVPNV
                     VMTVGLQGSGKTTSTGKIANWLAKNQERKPLLVSVDVYRPAAREQLKVVAGAVGQAIY
                     EDRTTDDPLTLVRGAVRYAQEKGYDTLMIDTAGRLHIDDELMVELEQIKAETKPVEVL
                     FVADAMTGQDAVRSAEEFHRRVGITGVILTKMDGDARGGAALSIKQVIGQPVKFVGVG
                     EKYDAIEPFYPDRIAQRILGMGDVLSLIEEVQGKIDQTEAEEQLLKMQKNQFTLEDFR
                     NQLRQFKKLGSMSKLMKMLPEQLMGGLQLSDEQSEQVEVQMKRTEAIIDSMTKAERAN
                     YKILDASRKTRIANGSGSTISEVNGLIRQYEQMRKMMQQMNRGGLFGGLGKKMMGGLG
                     GGLGGMLGGGLGGLLGGGNGGAEDFDNEYEETKDSLAKRLKKKKRHKKKR"
     gene            2330..2641
                     /locus_tag="VGB68_04400"
     CDS             2330..2641
                     /locus_tag="VGB68_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048503.1"
                     /translation="MRVKLYSLGLGMILASLAFSACGGTESNGVVNSTNATRTNSNNT
                     AVVTNSNTNAVITTTNSNTNMGNSNMGANNTMRNSNMNTNSNMGTNSGNTNMRNSNTN
                     R"
     gene            2853..3431
                     /locus_tag="VGB68_04405"
     CDS             2853..3431
                     /locus_tag="VGB68_04405"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY0048504.1"
                     /translation="MKVKLLSLGLGTIIAAVAFSGCGGTESNGIVNSTNANRSNNPNT
                     ASVVNANNTTVVSTTNNTAANRWANANGVTREQYDKERADYEREKGASNIGQGANDSW
                     IWFKTRAALLGTSDLRESTVNVDVANDVVTLKGTVETAAQKTKAEQVAKGIDGVKSVK
                     NELKVAPDDSMANVSAGNSANTKGSNANTNKK"
     gene            complement(3513..>4599)
                     /locus_tag="VGB68_04410"
     CDS             complement(3513..>4599)
                     /locus_tag="VGB68_04410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEY0048505.1"
                     /translation="VVRLRSIGDTVLATPSLDALRQFLPAARIDILLEDWVAPVLEGF
                     GAVDNVLSFKKDDFRSRLQAARQIRRAKYDVAFNLHGGTTATFFVRASGARHRVGYAE
                     NQYNFLYNHLLSSSSDFWQKEKTHSAEQQLALLGFVGVPVADKPKSRLGVTEKALSSL
                     EKKINAKMQSGGGEAKIFFPAGDFDESAIGNPRSAFALLHPVAAFETKQWATENFARI
                     ADFLHRKALPVVAVATRKESEVLEKLKRLSSFPILTFDDLTLPEITALASKAKLFVGN
                     DSGIAHIAAAVGTPSVVIFGSSNIHHWRPWTNAPNEIVYEPFACQPCAGYFCKEFGEP
                     QCISSVKVENVIQATEKVFERAFCKFG"
BASE COUNT         1102 a         1238 c         1101 g         1158 t
ORIGIN      
        1 aggcgaccca tttcgcccgc gccgaccttc acggaaataa cggtcgtgtt ggcgctgtcg
       61 cccggttttt ccgacacttc gaccgcatcg gcgttgccga ccagagcttt gacgagtttt
      121 tcgacggctt ctttcataag tttaccgaga gtttaccgag ttgactgaaa gttgactgag
      181 ttgactgagt taaaaacttt ctttaactca gtcaactcag tcaactcgct aaactcacaa
      241 ctattcttcc gttgccgctg ctgccggatt gtttttaatc aggctcgcca cggtttcggt
      301 cggctgcgcg ccgacgctaa tccagtattg aatgcggtcg tgtttcaaat tcacttccgc
      361 cggattcagc atcggattat acgtgccgac gttttcgatg tatttcccgt cgcgcttcga
      421 gcgcttttct ttaaccacga ggcgataaaa aggttttccc ttcgcgccca ttctcattaa
      481 actgattgct aacatatttt ctccgtaaat cgtcaatcgt caatcgtaaa tcgccgtgtc
      541 tctacaattc acgatttacg attttctatt atcgtttctt tttgtgtctc ttctttttct
      601 ttaatctctt tgccaaagaa tctttcgttt cttcgtattc gttatcaaaa tcttccgcgc
      661 cgccgtttcc gccgcctaaa agtccgccca agccgccgcc cagcattccg ccgagcccgc
      721 cgcccaaacc gcccatcatt tttttgccga gtccgccgaa aagtccgccg cgattcatct
      781 gctgcatcat tttgcgcatt tgttcatact ggcgaatcag cccgttgact tcggaaatcg
      841 tcgagcccga accgttggcg atgcgcgttt tgcggctcgc gtcgagaatt ttgtaattgg
      901 cgcgctcggc tttggtcatc gagtcgataa tcgcttcggt gcgcttcatc tggacttcga
      961 cctgctcgga ttgctcgtcg ctcaattgca gaccgcccat caattgttcg ggcagcatct
     1021 tcatcaattt cgacatcgag ccgagctttt taaactggcg gagctgattg cgaaaatctt
     1081 ccagcgtaaa ctggtttttc tgcattttca gcaattgctc ttcggcttcg gtctggtcga
     1141 tttttccctg aacttcttcg atgagcgata aaacgtcgcc cattccgagg attctctgcg
     1201 cgattctatc cggataaaaa ggctcaatcg cgtcgtattt ttcgccgacg ccgacaaatt
     1261 ttaccggctg accgataacc tgtttgattg ataaagccgc gccgccgcgc gcgtcgccgt
     1321 ccattttcgt caaaatcacg cccgtgattc cgactctccg gtgaaattct tcggctgaac
     1381 gaaccgcatc ctgcccggtc atcgcatcgg cgacaaacaa aacttcgacc ggtttggttt
     1441 ccgcctttat ttgttccagc tcgaccatca gctcgtcgtc gatgtgcagc cgaccggcgg
     1501 tgtcgatcat cagcgtgtcg tagcctttct cctgcgcgta acgcaccgcg ccgcgcacca
     1561 gagtcaacgg gtcgtcggtc gttctgtctt cgtaaatcgc ctgaccgacc gcgcccgcga
     1621 cgacttttaa ctgctcgcgc gccgccggac gataaacgtc gaccgaaacc agaagcggct
     1681 ttctttcctg atttttcgcc agccagttgg cgatttttcc cgtcgaggtc gttttgcccg
     1741 agccttgcag accgaccgtc atcacgacat tcggcacggc gcgcgtaaaa accagccgcg
     1801 aagaagtccc gcccattaat tcgaccagct cgtcgtagac gattttgacg acctgctcgt
     1861 gcggtttcag gtcttgccag acttcctgac cttcggcttt ggaacggacg gattcgacaa
     1921 aatccttcgc caccttataa ttaacgtcgg cttcgagcag cgccatacgg atttcgcgca
     1981 gggcgacatc gacgtgttcg ggcgtgagtt tgccttcacc gcgcaggttt ttgagagttt
     2041 tcttaagttt gtcggacagc gactcgaaca tatgtatttt tttgtttcgt cagtgtcttg
     2101 ttttaattca caaattaaac tttccaagtg ctacacagag acgagcggcg gtaatagccg
     2161 aaattttaga tgatagatgc cgcgcctctc gatgtcaaga aacgagcctt aaatatggct
     2221 gaaaccgccg ttatttcgtg atttttcttt taacggaaca aaacttgccg taattgcggc
     2281 gtttgacaaa aacgcccgca tttttgtcaa atgaatttga ggaggaatta tgagagtcaa
     2341 attatatagt ttaggattag gaatgatttt agcgtcgctc gctttttcgg cgtgcggcgg
     2401 aaccgagtca aacggcgtgg tcaattcgac caatgccacg cgaaccaaca gcaataacac
     2461 tgcggttgtt acgaacagca acacgaacgc cgtcattacg acgacgaaca gcaacacgaa
     2521 tatgggcaac tcgaatatgg gcgccaacaa cactatgaga aattcgaata tgaatacgaa
     2581 ttcgaatatg ggcacaaatt cgggcaacac gaatatgaga aattcgaata cgaaccggta
     2641 acggtttaat gaatttcgca tatatattcc agcgggttta aaagcccgcc tttttgtttt
     2701 gccggacaac tttttcgatt aacggaaagg ctttttttgc tttttcgttt ttgttaattt
     2761 ctccaatgga acgaaatttg ccataattca cttgttttta aaaaatttgt atttttttaa
     2821 gacaaacaac aggagagtct ttaggaggac aaatgaaagt caaattactt agtttaggct
     2881 taggcacaat tatagccgcg gtcgcttttt caggatgcgg cggaacggaa tcgaacggta
     2941 tagtcaacag cacgaacgcc aatcggtcga ataatccgaa cacggcgagc gtcgtcaacg
     3001 ccaacaacac gacagtggtc agcacgacca acaacaccgc tgccaaccgc tgggcgaacg
     3061 ccaacggcgt gacgcgcgaa caatacgata aggaacgcgc cgattacgaa agagagaaag
     3121 gcgccagcaa catcggtcag ggcgcaaacg acagctggat ttggtttaaa acgcgcgccg
     3181 cattgctggg cacgagcgat ttgcgcgaat caaccgtcaa tgtcgatgtc gccaacgacg
     3241 tcgtgacctt gaaaggaacg gtcgaaaccg ccgcgcagaa aacaaaagcc gaacaggttg
     3301 ccaaaggcat cgacggcgtg aaaagcgtga aaaacgaatt gaaagtcgcg cccgacgatt
     3361 cgatggcgaa cgtcagcgcc ggaaattccg ccaacacgaa aggctccaac gccaatacga
     3421 acaaaaaata agttcagaat ttaatttgaa cgctgaaggc gagtttcaat cgagactcgc
     3481 ctttttattg tcccgattca ttttcaaaaa aatcaaccga acttacaaaa cgccctttcg
     3541 aaaacctttt ccgtcgcctg aatcacattt tcgactttaa cgctcgaaat acactgcggc
     3601 tcgccgaatt ctttgcagaa ataaccggcg cacggctggc aggcgaaagg ctcgtaaacg
     3661 atttcgttcg gggcgtttgt ccacggtcgc cagtgatgaa tgttcgacga gccgaaaatg
     3721 acgaccgaag gcgtcccgac agccgccgcg atgtgcgcga tgcccgaatc gtttccgaca
     3781 aacaatttgg ctttcgaggc taaagccgtg atttccggca gggttaaatc gtcaaaagtg
     3841 agaatcggaa atgatgaaag ccgtttcagc ttttccagga cttcgctttc ttttctggtg
     3901 gcgacggcga cgaccggcaa agcttttcgg tgcagaaaat ccgcaatccg cgcaaaattt
     3961 tccgtcgccc attgcttcgt ctcaaacgcg gcgaccggat gaagcagggc gaacgcggag
     4021 cgcggattgc cgattgcgga ttcgtcgaaa tcgcccgccg gaaaaaaaat ctttgcctcg
     4081 ccgccgccgc tctgcatctt tgcgttaatt tttttttcca gagagcttaa agctttttcc
     4141 gtgacgccta ggcggctttt cggcttgtcc gcgaccggca cgccgacgaa tcccaaaagc
     4201 gcgagctgct gctcggcgga atgagttttt tccttctgcc agaaatccga cgacgaggaa
     4261 agcaggtgat tataaagaaa attatattgg ttttccgcgt agccgacgcg gtggcgcgcg
     4321 ccgctcgccc gcacaaaaaa cgtcgccgtc gttccgccgt gaagattgaa agcgacatca
     4381 tatttcgcgc gccgaatctg ccgcgccgct tgcagacgcg agcgaaaatc gtcttttttg
     4441 aagctcaaaa cattgtcgac cgcgccgaag ccttccagaa cgggcgcaac ccaatcttcg
     4501 agcagaatat caatccgcgc cgcaggcaga aattgccgca gagcgtccag cgaaggcgtc
     4561 gcgagaaccg tatcgccgat tgagcgcagc cgcacgacg
//
LOCUS       DASWNE010000218         2514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_183146, whole genome shotgun sequence.
ACCESSION   DASWNE010000218 DASWNE010000000
VERSION     DASWNE010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183146"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="VGB68_04415"
     CDS             <1..918
                     /locus_tag="VGB68_04415"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0048506.1"
                     /translation="ERSSHTVPMPRGGGLVIVLVCLLFYSIYTIFTAGNFSFGYFAGA
                     VLIAVISWFDDLKSVSSALRFLVHAIGAALAIFFLGYWQKIYLPLTGEIDFGLFGAIF
                     TFLWIIWLTNAYNFMDGIDGIAGTQGLTAGTGWLLVGLFFNLEITSFLGGVVALSNLG
                     FLMHNWQPAKIFMGDVGSAFLGFTFAVIPLLAANEMQSGGQPRLAAAAVWLVWLFVFD
                     TLYTFFRRLLHGEKFWQAHRSHLYQRLVISGHSHGFVTILYGILSVVILSAVLFWLKT
                     GNQLDALPSLLVLAIGGGIIILCYGKENS"
     gene            966..1850
                     /locus_tag="VGB68_04420"
     CDS             966..1850
                     /locus_tag="VGB68_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048507.1"
                     /translation="MGRAKDFLVNSDKKTNLPIRDWQKDAEFDVYIESYGVKIRISAH
                     EREAFQTIEKILQAILPDCFRIIEKTYVEHHFTLVWDADGKDSLYLNEKEVPSDHTRK
                     DVLRGFETQLRLTVAEYAPQRVFVHAGVVAWKDLAIVIPARSFQGKTSLVAALVKRGA
                     IYYSDEYAIFDADGYVYPFPKTLSIRGEIDEFTQKEYPVEAFGGKAGEKKIPVGMILI
                     TGFKPGAEWKPQRLSAGQGILEILEHVVPIRYNPKFSIKVLNQVANRAIIAKTLRGDV
                     SKSTDLILDFFESECIKN"
     gene            1902..2393
                     /locus_tag="VGB68_04425"
     CDS             1902..2393
                     /locus_tag="VGB68_04425"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEY0048508.1"
                     /translation="MSNSQNPIARQEGLVIQEMPNEVLVYDTNTNKAHCLNQTAAFIW
                     KSCDGKHSVKDISSLVGSEFGGAVNEDFVWLAIDQLNKDNLLQEKVDSKFEGVSRRDV
                     IRKIGLASVIALPVVASLAVPNTVLAVASCANVPCNLTGAPPVTCNSPCVCSQPTGPG
                     ICQ"
BASE COUNT          628 a          543 c          649 g          694 t
ORIGIN      
        1 gaaagaagct cgcacacggt gccgatgccg cgcggcggcg gactcgttat cgttctggtt
       61 tgtcttttgt tttattcgat ttacacgatt tttactgccg gcaatttttc tttcggatat
      121 tttgccggag cagttttgat agcggtcatc agctggtttg acgatttaaa atcggtttct
      181 tcggctttgc gttttctggt tcacgcgatt ggcgcggcgc tggcgatttt ttttctggga
      241 tactggcaaa aaatttatct tccgttgaca ggagaaatcg atttcggatt gttcggcgcc
      301 atcttcacct ttttatggat tatatggctg accaacgcct acaactttat ggacgggatt
      361 gacggcatcg ccggaacgca gggcttgacc gccggcaccg gttggctttt ggtcggtctt
      421 tttttcaatc tggaaattac aagctttctg ggcggagtcg tggctttgtc gaatctcgga
      481 tttctaatgc ataactggca gcccgcgaaa atatttatgg gcgatgtcgg gagcgccttt
      541 ctgggcttta cgtttgccgt tattccgctg ttggcggcga atgaaatgca gagcggcggt
      601 cagccgcgtc tggcggcggc ggcggtctgg ctggtctggc tgtttgtttt cgacacttta
      661 tatacgtttt tcagaaggct tctgcacggc gagaaattct ggcaggcgca ccgctcgcat
      721 ctgtatcaga ggctggtcat ctcgggacat tctcacgggt ttgtcacgat tctctacgga
      781 attttatccg ttgtaatttt atcggcggtt ttattctggc tgaaaaccgg aaaccagctc
      841 gacgctttgc cgtctttact cgttttggca atcggcggcg gcataataat tttatgttac
      901 ggcaaggaaa atagttgacg taaatcgaat aaaatgttaa tttaacgaat aattagaaac
      961 agctaatggg aagagcaaaa gattttcttg tcaattcgga taaaaagacg aaccttccaa
     1021 tccgggattg gcaaaaagat gctgaattcg atgtttatat cgagtcttac ggcgtaaaaa
     1081 ttcgcatcag cgcccacgag cgggaagcct ttcagacgat tgaaaaaatt ctacaggcaa
     1141 ttttgcccga ttgtttccgg ataattgaaa aaacgtatgt cgagcatcat tttacgctgg
     1201 tttgggacgc ggacggaaaa gattcgctct acctgaacga aaaagaggtt ccgagcgacc
     1261 atacccggaa agatgttctc agaggcttcg agacgcagct gcggctgacc gtggcggaat
     1321 atgcgcccca gagagttttc gttcacgccg gagtcgtcgc ctggaaggat ttggcgattg
     1381 tcataccggc gcgcagcttt cagggaaaaa cctcgctggt cgccgcgctg gtcaaacgcg
     1441 gagcgattta ttattcggac gagtacgcca ttttcgatgc ggacggctat gtttatcctt
     1501 ttccgaaaac tctttcaatc aggggcgaaa ttgacgaatt tacgcagaaa gagtatccgg
     1561 tcgaggcatt cggcggcaag gctggcgaaa aaaagatacc cgtcgggatg attctgataa
     1621 ccggatttaa gccgggcgcg gagtggaaac cgcagcgact gagcgccggg cagggaatac
     1681 tggaaatatt ggaacacgtc gttccaatca ggtataatcc aaaattttcc attaaagtct
     1741 tgaatcaagt ggcgaatcgt gccataatcg caaagacttt acgtggtgat gtctcaaagt
     1801 caacggattt aatcttggat ttttttgaat ccgagtgtat aaaaaattag tatttgcaat
     1861 tttggtctaa aaaacttgtc ttatttgccg gaggagaaat tatgagtaac tcgcagaatc
     1921 caatcgctcg tcaggaagga ttggtgatac aggaaatgcc caacgaagtt ttagtttacg
     1981 acacgaacac caataaagcg cattgtttaa atcaaacagc ggcttttatt tggaaatcct
     2041 gcgacggaaa acattccgtc aaagatattt ccagcctcgt cggaagcgaa ttcggcggtg
     2101 cggtcaatga agattttgtc tggctggcga tcgaccagtt aaacaaagat aaccttttac
     2161 aggaaaaagt cgattccaag tttgaaggcg tttccagacg cgacgtcatt agaaagattg
     2221 gtctggcgtc cgttatagca ttgccggtcg tcgcttctct agccgtgccg aatacggttt
     2281 tagcggttgc ttcttgcgcc aacgtgcctt gtaacttgac cggcgcgccg cctgtcacgt
     2341 gtaattcgcc gtgcgtctgc agccagccga ccggtcccgg aatttgccag taagagcgcg
     2401 tccaataaaa aaacagcgaa gagcggtgag aaattaactc tcaccgctct ttcattttga
     2461 aatatttatc catagaatgg ataaaaaaga tggtttcttc agaactcagc aaag
//
LOCUS       DASWNE010000219         4150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_218326, whole genome shotgun sequence.
ACCESSION   DASWNE010000219 DASWNE010000000
VERSION     DASWNE010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218326"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..2135
                     /locus_tag="VGB68_04430"
     CDS             105..2135
                     /locus_tag="VGB68_04430"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HEY0048509.1"
                     /translation="MPKNKDTRDFNFNRVTASNLNPQKSAVDVSTALPGEHSVRIQRV
                     NSFTGSPADVQSINARSAVSLDSAGETPNDAALINEALNHVDSISPALGFMSGQKAEF
                     VPDSFVKKTSAGTRIVNLQQQYKGIPVFSMERAVIFGNDGEVKNTTGTNVDLPPALET
                     YPVISVQTAARAAAEYMSSPSDEIDPWTKEPLPEIEIDVSHYEPEVLGQINLPSQPAV
                     LAKGVFGESIPAHLVLFYQGAATRLGWHFVISMPDMTEQYVIIVEADSQTANREKPEI
                     LYAQKTSSHMAAKGKVWIHNPGVSPQRALVDFPLDVSVYPLDASSLNLPDGFPQAWVD
                     DGGSAAIGNYTIAVMGNTADSISGSSVDGVLTFDPGDTEARGDVQKVINIFYFCNYMH
                     DFFYMLGFDERHGNFQKINFTGIGAGNDPVLARAHPGPVRGTANMLTRADGVRAEMNM
                     GLVGGIDRHTAFDSDVVFHEFAHGVTNRLVGGRFDGRALQQPQSRGMGEGWSDYFALT
                     IQNCIALIQNPDADEKTVTGDWVVARAEGIRLAPYDEFYPGTYGSLGISPYNEEHAVG
                     EIWCAALMKMNRDFGAALGDRVRGHRIGWQIVVDGLKLTPSNPSFLDARDALLRALED
                     LGRTGSLTEDEFFTLNRAAWAAFARFGMGPNANSIGASLDGIFEDTFPPEGI"
     gene            2312..>4150
                     /locus_tag="VGB68_04435"
     CDS             2312..>4150
                     /locus_tag="VGB68_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEY0048510.1"
                     /translation="MEVPRDAESQAFEEAKRNIRDFYASARVPQISGSGTRALEAWFL
                     GPKAENADILERLIVEAIRDQAFWRRNYHPEDPFHITEKIRQSDDYLAAVDSLNDGYR
                     QLLAFLKKSVPFFSMRYQGHMNWELTIPGILGYFAAMLYNPNNVAFEGSTATTLLEIL
                     VGDDLCRMFEFMDDSGIEPKIRPWGHITCDGTVANIEAIWSARNLKMFPLALKAALED
                     DALKAAKDIEINLAGGERKPLRSLDTWQLLNLKGDDVLEMPARISAEFPAIKRDAVTK
                     ALEKYSIQQLGFQNFSRRFLSEIEHAPIFTVPGTKHYSFPKAAALLGIGAANMIDIPV
                     DIDARLDVDKLREKLNECLERKIPVMTVVAVIGTTEESGVDPIKKVLELREEFRRKGL
                     DFTVHADAAWGGYHASVVREDFDMAPLPKGFVADLPLAAPLSGYVTEQFKSLRYVDSI
                     TVDPHKSGYIPYPAGALCYRNSAMRDLVTFSAPVVFHGEAEPTVGIYGVEGSKPGAAA
                     AAVYLSHRVIRPSRSGYGKIISQALYSCKRLYVRLVCMPGAEDGFVIVPVPNLERVEG
                     VPGDNLQEKIAFISEKINKKTNAEIAADTDVSSLLPELGPDQNILAY"
BASE COUNT         1008 a         1068 c         1152 g          922 t
ORIGIN      
        1 tccgccgacc gccagataga ttacgaatat gccgccgaca ttgcgcgcat cattgccggc
       61 gccgcactga tagccgccga catttagaaa taaaaggaga tattatgcct aaaaataaag
      121 acacccgcga ttttaatttt aaccgtgtaa ctgcttcgaa tcttaatccg caaaaatctg
      181 ccgttgacgt ttcgaccgcc ttgccgggcg agcacagcgt cagaatccag cgcgtcaata
      241 gttttacggg cagccccgcc gacgttcaat cgattaacgc ccgcagcgcc gtctcgctcg
      301 attcggcggg agaaactccg aatgatgccg ccctgataaa tgaggcgttg aatcacgtcg
      361 attcgatatc tccggcattg ggttttatgt ccggacagaa agcggaattc gttcccgact
      421 cattcgttaa aaaaacgagc gccggaacgc gcatcgtcaa tttacaacag caatataaag
      481 gcattccggt attttccatg gagcgggcgg ttattttcgg caatgacggc gaagtcaaaa
      541 acacgaccgg aaccaatgtc gacctgcctc cggctttaga gacgtatccg gtcataagcg
      601 tgcagacggc agcgcgggcg gcggcggaat atatgagcag tccgagcgat gagatagacc
      661 cctggacgaa agagcctcta ccggaaatag agattgacgt cagccattac gagccggaag
      721 ttttaggaca aattaatttg ccgtcgcagc cggcggtgct cgccaaaggc gtgttcggcg
      781 aaagcattcc ggcgcacctg gttttatttt atcagggcgc ggcgacgcgg ctcgggtggc
      841 atttcgtcat cagcatgccg gatatgacgg agcaatacgt gattatcgtc gaagccgatt
      901 cgcaaacggc aaaccgcgaa aaacccgaaa ttttatacgc gcagaaaact tcgagtcata
      961 tggcggcgaa aggaaaagtc tggattcata atccgggagt cagcccgcaa agagccctgg
     1021 tggattttcc tttggacgtc tcggtttatc cgctggacgc ttcatctttg aatttgccgg
     1081 acggatttcc gcaggcgtgg gtcgatgacg gcggctcggc ggctatcgga aactacacaa
     1141 tcgcggtgat gggaaacacc gcggattcaa tcagcggttc gagcgtcgat ggagttttaa
     1201 ctttcgaccc cggcgacacg gaagctcgcg gagacgtgca gaaagtcatt aacatttttt
     1261 atttctgcaa ttatatgcac gatttctttt atatgctcgg atttgacgag cgccacggca
     1321 atttccagaa aattaatttt accggaatcg gagccggcaa cgaccctgtt ttagcccgcg
     1381 ctcatccggg tccggtgcgc ggaacggcaa atatgctgac gcgcgccgac ggcgttcggg
     1441 ctgaaatgaa tatgggtttg gtcggcggca tcgaccgcca cacggctttt gattcggacg
     1501 ttgtttttca cgaattcgca cacggcgtca ccaaccggct ggtcggcggc agattcgacg
     1561 gtcgcgctct gcagcagccg cagagccgcg gaatgggcga aggctggagc gattatttcg
     1621 ctctgaccat tcaaaactgt atcgcgctga ttcaaaaccc ggatgccgac gaaaaaaccg
     1681 tgaccggcga ctgggtcgtc gcccgcgccg aaggaatcag attggctccg tatgatgaat
     1741 tttatccggg cacctacggc agcctcggca tctcgcctta taacgaagaa cacgcggtcg
     1801 gcgaaatctg gtgcgcggct ctgatgaaaa tgaatcggga tttcggcgct gccctcggcg
     1861 acagagtcag aggacatcga atcggctggc agattgtagt agacggtctg aaactgacgc
     1921 cttcgaatcc cagttttctg gacgccagag acgccctttt acgcgctctg gaagacctcg
     1981 gaagaaccgg ctcgctgacc gaagatgaat ttttcacgct aaaccgggcg gcgtgggcgg
     2041 cgtttgcccg tttcggtatg ggaccgaatg cgaactctat cggggcgtcg ctggacggaa
     2101 ttttcgaaga cacgtttccg ccggaaggta tttaaagcgc cggtgccgga aattaaataa
     2161 gagaaaaata tctttggaat agattgcaga taacagatga taacggatag attttatctt
     2221 tcatatttga gccgtcatct atcccgaaga aacaggtttt tatcataatt cgggaaaaac
     2281 tgccggattt attaaaagga gctgaaaaga aatggaagtg cctcgtgacg ccgaaagtca
     2341 agcgtttgaa gaagcgaaaa gaaatatacg ggatttttac gccagcgcgc gggtgccgca
     2401 gataagcggc agcgggacgc gcgcgctgga agcgtggttt ctcggtccaa aggcggaaaa
     2461 cgccgatatt ctcgaacgat taatcgtcga agccattcgc gaccaggcgt tctggcggcg
     2521 aaattatcac ccggaagacc cgtttcacat caccgaaaaa atacgccaat cggacgatta
     2581 tctcgccgcc gtcgattctt taaatgacgg gtatcggcag cttctggcgt ttctcaaaaa
     2641 atccgttcct tttttcagta tgcgctatca ggggcatatg aactgggaac tgacgatacc
     2701 gggaatcctg ggttattttg cggcgatgct ctataacccg aacaacgtgg cttttgaagg
     2761 ctcgaccgcg acgaccttac tggaaattct cgtcggcgat gatttatgcc ggatgtttga
     2821 atttatggac gattccggca tcgagccgaa aattcgcccg tggggtcata tcacctgcga
     2881 cggaacggtc gccaatatcg aggcaatctg gtcggcgaga aatctgaaga tgtttccgct
     2941 cgccctgaaa gccgcgctgg aagacgacgc gctaaaagcg gcgaaagaca tcgaaatcaa
     3001 tctcgccggc ggagagcgca agccgctccg ctcgctcgac acgtggcaat tgcttaatct
     3061 gaaaggcgac gacgttttag agatgccggc gcggataagc gcggaatttc ctgccataaa
     3121 aagagatgcg gtcaccaaag cgctggagaa atattcgatt cagcagctcg gatttcagaa
     3181 tttttcccgc cgttttcttt ccgaaatcga gcacgcgccg atttttaccg tgcccggcac
     3241 caagcattat tcgtttccga aagcggcggc gctgctcggc atcggcgctg caaatatgat
     3301 tgacattccg gtcgatatcg acgcgcgcct ggacgtcgat aagctcaggg aaaaattaaa
     3361 cgaatgtctg gagcggaaaa ttccggtaat gaccgtcgtc gccgtcatcg gaacgaccga
     3421 ggaaagcggc gtcgacccga ttaaaaaagt tctggaatta agagaagagt ttcggcgcaa
     3481 aggattggat tttaccgtcc acgccgacgc cgcgtggggc ggctatcacg cttcggtcgt
     3541 ccgcgaagat ttcgatatgg cgccgctgcc gaaaggattt gtcgccgacc tgccgctcgc
     3601 cgcgcctttg agcggatatg ttaccgagca gtttaaatct ctcagatacg tcgattcgat
     3661 tacggtcgac ccgcataaat ccggctacat tccatatccc gccggagcgc tttgctatcg
     3721 caattccgcg atgcgcgatt tggttacttt cagcgcgccg gtcgtttttc acggcgaggc
     3781 ggagccgacc gtcggaattt acggcgtcga gggctcgaag ccgggcgcgg cggcggctgc
     3841 cgtctatctg agtcaccgcg ttattcgtcc ttccaggagc ggctacggga aaatcatcag
     3901 ccaggcgctt tacagctgta aaagattgta tgtccggctc gtttgtatgc cgggggcgga
     3961 agacggtttt gtcatcgttc cggttccgaa tcttgaacgc gtcgagggcg ttccgggcga
     4021 taatctgcaa gaaaaaatcg catttatcag cgagaaaatc aataaaaaga ccaacgccga
     4081 gattgccgcc gataccgacg tctcgtcgct gcttcccgag ctcggacccg accagaatat
     4141 tctggcttac
//
LOCUS       DASWNE010000220         2792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_642443, whole genome shotgun sequence.
ACCESSION   DASWNE010000220 DASWNE010000000
VERSION     DASWNE010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642443"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..627)
                     /locus_tag="VGB68_04440"
     CDS             complement(55..627)
                     /locus_tag="VGB68_04440"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HEY0048511.1"
                     /translation="MLRNNWKRKNFGDEDEAPREIKDIEKARRRTFDRAIRLLTFKWR
                     SIAELRERLLEKNWTNEEIVEHVIARLKEYGYLNDEQFAKDYAASKIRQKPQGKRILQ
                     QKLLLKKVPKETADAALEKVFEETPEADLVEQAIRKRIRLKGKPETREDTKKFYDFLL
                     RQGFSYDLVSGKMREIGKEELEQDGQDGQD"
     gene            712..1494
                     /locus_tag="VGB68_04445"
     CDS             712..1494
                     /locus_tag="VGB68_04445"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY0048512.1"
                     /translation="MLNEQIKLHENFGSAHLDVPRNVLVYLPPEYGLNPERRYPVLYM
                     HDGQNLMNPQDAFGGVAWGVDETAQKLILTGEIEPLIIVGIYNAGEKRIDEYTPVKSD
                     VGRMKGHGGKADFYGRMIIEELKPFIDRAYLTRPEREFTGIGGSSLGGLVSFHLGLKR
                     PDVFSRVAVMSPSVWWANNQIIREAAKIGERLPLRIWLDIGKREGSRIKHQVRALKEI
                     LLANGWKNGLDLAYLEIPEARHEEAAWAARFDHVLRFLFPVI"
     gene            1573..2511
                     /gene="truB"
                     /locus_tag="VGB68_04450"
     CDS             1573..2511
                     /gene="truB"
                     /locus_tag="VGB68_04450"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEY0048513.1"
                     /translation="MDGILIIDKPAGITSHDVVARCRRILKTKRIGHTGTLDPFATGA
                     VVILVGKATRLAQFLDKDAKEYEAVVRLGFETETGDRTGEIRNTKYELRNVSAAEIEE
                     VLKDFRGETEQTPPMYSAKKVEGKKLYELARKGVEIERKPVKITIYELELIESRQRSA
                     VSGQPEAEDGSPKTEDRNIRVACTAGTYIRTLAEDIGRKLETGAHLAELRRTRAGKFD
                     LSQAVTLERLEEIAAENRLEEVLISMSEALSHLPEIKLPDEEIRKTQNGMKLRFDASE
                     MLDNQPLRLTDKSENLIAVGFYSEAEKTIQPKVVLV"
BASE COUNT          698 a          686 c          739 g          669 t
ORIGIN      
        1 tctctaaact tctctagacc caaaattaaa acggtcaacc tggatgtttg ttttctaatc
       61 ctgtccatcc tgtccatcct gttctaattc ttctttgcca atctcgcgca ttttgccgct
      121 caccaaatcg taagaaaatc cttgccgcaa aagaaaatcg taaaattttt tcgtatcttc
      181 gcgcgtttcc ggtttgcctt ttaaacggat gcgttttcgg attgcctgct cgaccagatc
      241 ggcttcgggc gtttcttcaa aaactttttc gagcgcggcg tcggcggtct ctttcggaac
      301 ttttttcagc aggagctttt gctgcaaaat tcttttgccc tgcggtttct ggcggatttt
      361 cgaagcggcg taatcctttg caaactgctc gtcgttcaga taaccgtatt ctttgagcct
      421 ggcaatcacg tgctcgacaa tttcttcatt cgtccagttt ttttccagca ggcgctcgcg
      481 cagctcggca atcgagcgcc atttgaaggt cagcaggcga atggcgcggt caaacgtccg
      541 gcggcgcgcc ttctcgatgt ccttaatctc gcgcggcgct tcgtcctcgt cgccgaaatt
      601 ttttcgcttc cagttgtttc gcagcatgtc ttttattaat tataccgcta aatgcgcgaa
      661 aaagccggat ttggaaaacg cgcgccgaaa cgtgtaaggt aaaacttaaa tatgttaaac
      721 gaacaaatca agctgcacga aaatttcggt tcggcgcacc tcgacgtgcc gcgcaacgtt
      781 ctggtttatc tgccgcccga atacggtttg aacccggagc gccgctatcc ggttttgtat
      841 atgcacgacg ggcagaatct gatgaacccg caggacgctt tcggcggcgt cgcgtggggc
      901 gtggacgaaa cggcacaaaa actcatcctg acgggcgaaa tcgagccgct gattatcgtc
      961 ggcatttaca acgcgggcga aaaacgaatc gacgaataca cgcccgtcaa aagcgatgtc
     1021 ggcaggatga aaggtcacgg cggcaaagcc gatttttacg ggcgaatgat tatcgaggaa
     1081 ttaaagccgt ttatcgaccg cgcgtatctg acgcgccccg aacgcgaatt taccggcatc
     1141 ggcggctcaa gcctcggcgg tctggtctcg tttcatctcg gcttgaagcg tccggacgtt
     1201 ttcagccgcg tcgccgtgat gtcgccttcg gtctggtggg cgaacaatca gattatccgc
     1261 gaagcggcga aaatcggcga gcgtctgcca ttgcgaatct ggctcgacat cggcaagcgc
     1321 gaaggctcgc gcatcaagca ccaagtgcgc gcgctgaagg aaatcctgct ggcgaacggc
     1381 tggaaaaacg gcttggacct cgcttatctg gaaattccag aagcgcgtca cgaagaagcg
     1441 gcttgggcgg cgcgcttcga ccacgttttg cggtttttgt ttccggttat ttaatttctt
     1501 cgagtgaaaa ggtgaaaaag ttttgccgat ttgctcaatt actttttcac ttttaccctt
     1561 tttcactttt ttatggatgg aattttaatc atcgacaaac ccgccgggat tacttcgcac
     1621 gatgtcgtcg cgcgctgccg ccgaatttta aagacaaagc gcatcgggca taccggaacg
     1681 ctcgaccctt ttgcgacggg cgcggtcgtg attctggtcg gcaaggcgac cagactcgct
     1741 caatttctgg acaaggacgc gaaggaatac gaagccgtcg tgcgtctggg atttgaaacc
     1801 gagacgggcg accggacggg cgaaatacga aatacgaaat acgaattacg aaatgtttcc
     1861 gccgccgaaa tcgaagaggt tttaaaagat tttcgcggcg aaaccgagca aacgccgccg
     1921 atgtattccg ccaaaaaagt cgaaggcaaa aagctttacg agctggcgcg aaaaggcgtc
     1981 gagatcgagc gaaaacccgt aaaaattacg atttacgaat tggaattaat cgaaagccgt
     2041 cagcggtcag cggtcagcgg tcagccggag gcggaagacg gaagcccaaa gaccgaagac
     2101 cgaaatatac gcgtcgcctg caccgccgga acttacattc gcactttggc tgaagacatc
     2161 ggcagaaagc tcgaaaccgg agcgcatctc gccgaattga gaagaacgcg ggcgggaaaa
     2221 tttgatttgt cgcaagccgt gacgctggag agactggaag aaatcgccgc cgaaaacagg
     2281 ctcgaagaag ttttgatttc gatgagcgaa gccctttcgc acctgccgga aatcaagctc
     2341 ccggacgaag aaatcagaaa aacgcaaaac ggtatgaaac tgcgcttcga cgcttcggaa
     2401 atgctcgata atcaaccgct ccggctaacg gacaaaagcg aaaatctgat tgccgtcggt
     2461 ttttacagcg aagcggaaaa aactattcaa ccgaaagtgg ttttggtgta gaattaccgg
     2521 aagtttgact tttatttcgg atgcgtcgat tgtgatacga gtgttgaacg ctgaacgtta
     2581 aggttgttat taaacgggtt attcaaaatt cgagactcaa aaaattcaaa actttttata
     2641 gtgattatga aaaaaagata tttagcggcg ggcgccggct tgggagccat cggcggtttg
     2701 attgcgtgga aaatgctgac gcgggcgggc gaggttaatt gggacgacgt ttcggagctg
     2761 gttccgcacg cggaaaattc gcattttacg ga
//
LOCUS       DASWNE010000221         3176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_112596, whole genome shotgun sequence.
ACCESSION   DASWNE010000221 DASWNE010000000
VERSION     DASWNE010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112596"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            451..3102
                     /locus_tag="VGB68_04455"
     CDS             451..3102
                     /locus_tag="VGB68_04455"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HEY0048514.1"
                     /translation="MKNLLPGSVGNSIALLFFIFLSTNFVSAQINSTPPKASETTQPS
                     PSPALVSPETGTQNSILKTGSEPKTALSPYIPKTEPARIPRFEAPPVIDGQLNDAVWQ
                     TAAVFGDFVQTNPGDNVKPTHPTEFMMGYDSKNLYMAFRVRQDRDKVRATVARRDNIF
                     NDDYVLVHLDTFNDQRQAYLLFFNPLGIQADGTFTEGRGEDYSLDIIMESKGVLTEDG
                     FTVEIAIPFKSLRYEAGKNKLWGLHINRRVKYNNNEYNSWMPTNRSISGWLNQSGKIT
                     GLEGIETTRQLEINPSFTLSQTGRRTGFTFDGNPGGRFVNDGLKGDFGMTAKFSLTPT
                     ITLDFAYNPDFAQVEADAPVSTANVRFPIFFQEKRPFFLERNDIFQSGLNIVNTRAIV
                     DPDIAAKLTGRRGKNTFGLLYASDNAPGNYSKDEREELLICRREQLSDPTRICSIERF
                     VNKNAEIGVLRVKRDIGTQHNLGFFATTYHFPDLHNNTAGFDGRFRLDKKTVTDFQIV
                     ATNSRSCVFDADFEPALNDAQAKHNEKVCGSKFDEYRTGNGVGYRVYLERSDRNLYMN
                     YLAMGRTREYRADVGFTNRVDTNYLGSFIQYQTDRNAKKTIVFKRLSNESNVSYDWDG
                     RSQNIRSNTQGMLGFHRQTFLGLGAEYGYERVFEHEFGQKRTATQRGAFFGFDSERSA
                     HRKEIYGFIETTPFKQLFFFTVLSHSVGNLDYDFGAGPKFPRVSAPFLVWQARCAGIP
                     NCTEPRPGFDPGPGDQLFIESSIRYQPTTAWQMQLNYTRTRLTRHDTGRVAFDDNVYS
                     LRSTYQFTRDIFARMRLDYSNISTRFRPQLIFGWTPSPGTALYAGYNDDFNYNGFNPF
                     TRLPESGFRSNGRSFFIKASYLFRKNF"
BASE COUNT          833 a          826 c          747 g          770 t
ORIGIN      
        1 ttcagattat ttatcataat caaagactcc ttcaggtgaa accccgaaaa atataatttc
       61 cttgaatact aaattgattt cattagattg aattttcagg cttgcctgtt gtccgtataa
      121 aacgcttgaa atgagactgc cggagaaaag ttttcgaaaa ttttagagcg aaatatttat
      181 tcggaaaaaa caatttcgga gacgaccgcc tttgccgcat atgctgtatt ccgagcattc
      241 tatccccgcg gatgagcctt taatcacaaa agaatttatc ggcgtcagga attcagccta
      301 tatttaaaag gcttcaaatt tttaaacggg ttcaaattca cgaaaaaaaa tgtcataaaa
      361 caccagcccg cggggtttta ctgttgaaat cagttttata ccagcgaaaa gcccggataa
      421 aaattcaatt catcgagtat ttttaaattt atgaaaaact tacttccagg gtctgtcgga
      481 aattcgattg ctttattatt ctttatattc ctgagcacga attttgtttc cgcgcagatt
      541 aactcaacgc cgcccaaagc gagcgaaact actcagccgt cgccttctcc ggcgcttgtt
      601 tctcccgaga ccggaacgca aaattccatt cttaaaaccg gctctgagcc gaaaaccgcg
      661 ctctcgccgt atataccgaa aaccgagccc gcgcgaattc cgcgtttcga agcgccgccg
      721 gtgattgacg ggcagctaaa cgatgcggtc tggcaaacgg cagccgtttt cggcgatttc
      781 gtgcagacca atcccggcga taacgttaag ccgacgcatc cgaccgaatt tatgatgggc
      841 tatgattcaa agaatctgta tatggctttt cgcgtcaggc aggacagaga caaagtgcgc
      901 gctacggtcg cgcgccgcga caatatcttt aacgacgatt acgtactggt tcatctggat
      961 acttttaacg accagcgtca ggcttattta ttgtttttca atccgctcgg aatccaggcg
     1021 gacggaactt ttaccgaggg acgcggcgag gattacagcc tcgacatcat aatggaatca
     1081 aagggcgttt tgaccgaaga cggctttacc gtcgaaatcg ccatcccgtt caaatccctg
     1141 cgttacgagg caggcaaaaa taaactctgg ggtctgcata tcaatcgccg cgtcaaatac
     1201 aacaacaacg aatataactc gtggatgccg accaatcgca gcatctccgg ctggctcaac
     1261 caatccggaa aaatcacggg tctggaaggc atcgaaacga cgcggcagct ggaaattaat
     1321 ccgagcttta ctttaagcca gaccggcagg cgcacgggct ttaccttcga cggcaatccc
     1381 ggcgggcgat tcgtcaacga cggcttaaaa ggcgatttcg gaatgaccgc gaaatttagt
     1441 ctgacgccga ccattacgct cgattttgct tataatcccg attttgcgca ggtcgaagcc
     1501 gacgcgcccg tctcgaccgc caacgtgcgt ttcccgattt tctttcagga aaaacgccct
     1561 ttctttttag aaagaaacga tatttttcaa agcgggctga atatcgttaa cacgcgcgcc
     1621 atcgtcgacc cggacatcgc cgcgaaatta accggcaggc gcggaaaaaa tactttcggc
     1681 ttgctgtacg cttccgataa tgcgcccgga aattactcga aagacgaacg cgaagagctg
     1741 ctgatttgcc ggcgcgagca gctttcagac ccgacccgga tttgcagcat cgagcgcttc
     1801 gtcaacaaaa acgcggaaat cggcgtgctg cgggttaaac gcgatatcgg aacgcagcat
     1861 aatctgggat ttttcgccac gacttaccac tttcccgatt tgcataataa caccgccggt
     1921 tttgacgggc gatttcgttt agacaagaaa accgtgaccg actttcaaat cgtcgcgacc
     1981 aattcgcgca gttgtgtttt cgacgcggat tttgagccgg cgcttaacga cgcgcaggca
     2041 aaacataacg aaaaggtgtg cggcagcaag ttcgacgaat atcgcaccgg caacggcgtc
     2101 ggctacaggg tttatctcga acgctcggac agaaatttat atatgaatta tctggcaatg
     2161 ggcagaacca gagagtatcg cgccgacgtc ggctttacga atcgcgtcga tacaaactat
     2221 ctcggttcgt tcattcaata tcaaaccgac cgtaacgcga agaaaaccat tgtcttcaaa
     2281 cgtctttcca acgaaagcaa cgtcagctac gactgggacg ggcgcagtca aaacatccgc
     2341 tcgaatacgc agggaatgct cggctttcat cgacaaactt ttctcggctt gggtgcggaa
     2401 tacggttacg agcgcgtatt cgaacacgag ttcggacaga agcgcacggc aacgcagcgc
     2461 ggagcctttt tcggctttga ttcggaacgc tccgctcacc gcaaggaaat ttacggcttt
     2521 atcgagacca cgccgtttaa acaactgttt ttctttacgg ttttaagcca cagcgtcggc
     2581 aatctcgatt atgatttcgg cgcgggaccg aaatttccgc gcgtcagcgc gccgtttctc
     2641 gtctggcagg ctcgctgcgc gggcatcccg aattgcacgg aaccgcgtcc cggctttgac
     2701 cccggtccgg gcgaccagct tttcatcgaa tcttcaattc gttaccagcc gacgaccgcc
     2761 tggcagatgc agctgaatta cacgcgcacg cgtctgacgc gccacgacac cggcagggtc
     2821 gcttttgacg acaacgttta cagcctgcgc tcgacctatc aatttacgcg cgacattttt
     2881 gcccgtatgc gcctcgatta ttcaaacatt tcgacgcgct ttcgcccgca attgattttc
     2941 ggctggacgc cgagtcccgg cacggcgtta tacgcgggtt acaacgacga ctttaattat
     3001 aacggcttta atcctttcac gagattgccc gaatccggct ttcgcagcaa cggcaggtcg
     3061 ttttttatca aggcttccta cctgttcaga aagaattttt agatgccggg ataatctgac
     3121 tttatccgaa aggctattga taaattctga aatgaaaaag ggcgtgaaaa taaaaa
//
LOCUS       DASWNE010000222         4768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_430743, whole genome shotgun sequence.
ACCESSION   DASWNE010000222 DASWNE010000000
VERSION     DASWNE010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4768
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430743"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="VGB68_04460"
     CDS             complement(<1..1010)
                     /locus_tag="VGB68_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HEY0048515.1"
                     /translation="MREKSFFTKAVHAGENRTENYGAVSVPIYTSSVFAFTDADEGAA
                     IHNYQKEGYFYGRLGNPTQTALEKACAELENGEDAMAFASGMAAVSASVLSLVKTGEH
                     IVAPESMYSTTTSFLAHLAENFGIETTFIDATRAENYAAAARPNTKLFWIETPSNPLL
                     KITEFSTVVEIAEEKGIATVADNTFATPFNQRPLDFGVDAVIHSATKYFGGHSDLTAG
                     LMVGKREIVDRARHTIGKLYGGNIAPQVAWLVLRGIKTLALRMERHNHNAYAIANMLA
                     NHPKVKEVFYPALISHPQYEIARRQMKGFGGMISFDVGGVEEGKRLVNNVEVCTLATS
                     LGG"
     gene            complement(1024..1572)
                     /locus_tag="VGB68_04465"
     CDS             complement(1024..1572)
                     /locus_tag="VGB68_04465"
                     /inference="COORDINATES: protein motif:HMM:NF041414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HEY0048516.1"
                     /translation="MSNNTEIKTLKAHLALNVRNVEQSIEFYKKMLGIEPSKVRSGYA
                     KFDIYNPPLNLTLNQHPFNERGALSHLGIQVAATEDVLAMRERWIENGLVTQDEMQTN
                     CCYALQDKTWVYDPDGNEWEVFVVLEDNLAETSMCCAADETTAPIKLSAEEENPVDSA
                     ASCCAPAAVPVSINRVGEKSCC"
     gene            1688..2131
                     /locus_tag="VGB68_04470"
     CDS             1688..2131
                     /locus_tag="VGB68_04470"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY0048517.1"
                     /translation="MSEDFLFDLETFFMALADKTRLRLLNLMRQDEVCVCFFTEVLSE
                     SQPKISRHLAYLRNAGIVEARREGKWMHYRIIEPENDYAAQVLRDTLEWLKSHEKMQR
                     DYENLAIACCSPSAPVTITRAPKPNTFVETNIKNAEREQMETFLL"
     gene            complement(2123..2305)
                     /locus_tag="VGB68_04475"
     CDS             complement(2123..2305)
                     /locus_tag="VGB68_04475"
                     /inference="COORDINATES: protein motif:HMM:NF037605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5522 domain-containing protein"
                     /protein_id="HEY0048518.1"
                     /translation="MSLTKTKQTTKQEKPVEGVDYYFEDGLMVLTARFLLKRGCCCNN
                     GCRHCPYPKEDKNNLK"
     gene            complement(2274..3032)
                     /locus_tag="VGB68_04480"
     CDS             complement(2274..3032)
                     /locus_tag="VGB68_04480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEY0048519.1"
                     /translation="MKKERIDKLLVEQGFADSRTKAQALVMSGVVLVDECRVEKSSQE
                     FAAGSKIRIKGKTAESKYVGRGGLKLEKALQDFHITVNEYICLDIGASTGGFTDCLLQ
                     NGAKKVVTVDVGTNQMVWQLRNDARVEVRENTNARYLKPEDFDGKFDLIVMDVSFISV
                     TKIFPALPPLLKNSGKIITLIKPQFEVGRGEVGKGGIVREPEKHERVINEVNNRATQN
                     GLKVCGVVDSPILGADGNKEFLALYELDENKTNN"
     gene            3209..4165
                     /locus_tag="VGB68_04485"
     CDS             3209..4165
                     /locus_tag="VGB68_04485"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEY0048520.1"
                     /translation="MKRETKLSSFAKYAWFVLAYNVVVILWGVFLRASKSGDGCGQHW
                     LTCHGEVLPSAPQLKTVIEFSHRLTSALDGIFLIILVVWAFLIWRSNKSENGHLLKAA
                     VGSLVFVIIEGLIGAGLVLTGNTAETLTLARPFWMAGHLINTLILLTFLTLTAWFAVG
                     GKPFNFKAQPKVLLFLGIAVLGIFLVGMSGSVAALSSMLFPSETLTEGMTKDFSAASH
                     ILLRLRISHPILSIMVAVFLVFLAGWLKSRAMGNYRINRWANVLTVLIVIQIAFGALT
                     LLTLAPIVMQIGHLFLADAVWIVFVLLSASFLAEQNELPEVS"
     gene            complement(4271..4711)
                     /locus_tag="VGB68_04490"
     CDS             complement(4271..4711)
                     /locus_tag="VGB68_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048521.1"
                     /translation="MKICPNCKKQYADNAAVCPDDGTFLDLETPAIDTQTYESAADTV
                     PHTADSVDRIGTAAAETFDRDAGAFGAGMEPLGDRAATTTAFSETGDLAGDDYSENPL
                     FGWLVPLIIVAVLLVLGFMFCSKPVVPTAKADEIWMNCAKDKLI"
BASE COUNT         1127 a         1173 c         1110 g         1358 t
ORIGIN      
        1 ccgccgagcg atgttgccag cgtacagact tccacattat tgaccaagcg ttttccttct
       61 tcgacgccgc cgacatcaaa agaaatcatt ccgccaaagc ctttcatctg tcgccttgcg
      121 atttcgtatt gcggatgaga aatcaaagcc ggataaaaaa cttctttgac tttcgggtga
      181 tttgccaaca tattcgctat agcatatgcg ttatgattgt gacgctccat tctcaaagcc
      241 agagttttga ttccgcgcaa aacgagccac gcaacctgcg gcgcgatatt gccgccgtaa
      301 agcttgccga ttgtatgacg cgcacggtcg acgatttcac gttttccgac catcagaccg
      361 gcagtcaaat cgctgtgtcc gccaaaatat ttggttgcgc tgtgaatgac ggcatcgact
      421 ccgaaatcga gcgggcgctg gttgaaaggc gtcgcaaaag tattgtcggc gacggtggcg
      481 attccttttt cctctgcgat ttccacaact gtggaaaact ctgtgatttt caaaagcgga
      541 ttggaaggcg tttcaatcca gaacaatttt gtattcggtc gcgcggcggc ggcataattt
      601 tccgctctgg tcgcatcgat aaatgttgtt tcgattccga aattttcggc tagatgcgcc
      661 agaaaactgg tcgtcgtcga atacatcgat tcgggcgcga cgatatgctc gccggtttta
      721 accaaactta aaaccgaagc ggaaaccgcc gccattcccg acgcgaaagc catcgcgtct
      781 tcgccgtttt cgagctcggc gcaagccttt tccagcgctg tttgcgtcgg atttccgagc
      841 cgcccgtaaa aataaccttc tttttgataa ttgtgaatcg ccgcgccttc gtccgcgtcg
      901 gtgaaggcga aaaccgacga agtataaatc ggaacgctga ccgcgccgta attctccgtt
      961 cgattttcgc ccgcgtgaac cgctttagta aaaaatgatt tctcccgcat ccgtctctcc
     1021 aaattagcag caagattttt cgccgacgcg attgatgcta accggaactg ccgccggagc
     1081 gcaacacgac gcagcggaat cgaccggatt ttcttcttcc gccgataatt taatcggcgc
     1141 ggtcgtttca tccgccgcgc agcacatcga agtttccgcc agattgtctt ccagaacgac
     1201 gaaaacttcc cattcattgc cgtccgggtc atagacccac gttttatcct gaagcgcata
     1261 acagcaattc gtctgcattt cgtcctgcgt caccaagccg ttttcaatcc accgctcgcg
     1321 catcgccaga acgtcttcgg tggcggcaac ctgtatgccg agatgcgata acgcgccgcg
     1381 ttcattgaat ggatgttgat tcagcgttaa atttaagggc ggattataaa tgtcgaattt
     1441 ggcgtagccg ctgcgaactt tcgacggttc gatgccgagc atttttttgt aaaactcgat
     1501 gctttgctcg acattcctga cgttgagcgc caggtgcgct tttaatgttt taatttccgt
     1561 gttatttgac ataatttctt tctcctttga tattcatata cgttttaacg tatatgtcct
     1621 cttctcttgt aacattcgaa aaattaatag tcaagacatt tttgcgaaaa tgtgataaat
     1681 tttttttatg agcgaagatt ttcttttcga tttggaaact ttttttatgg ctctggcgga
     1741 caaaacgcgg ctgcgtcttt tgaatctgat gcggcaggac gaggtctgcg tttgtttttt
     1801 cactgaagtg ttaagtgaaa gccagccaaa gatttcgcgg catctggcat atctgcgaaa
     1861 cgccggaatc gtcgaggcgc gacgcgaagg gaaatggatg cattatcgca ttatagaacc
     1921 ggaaaacgat tacgccgcgc aggttttgcg cgacacgctg gaatggctga aatcgcacga
     1981 aaaaatgcag agagattatg aaaacctggc aatcgcctgt tgctcgccga gcgcgcccgt
     2041 caccatcacg cgcgccccga aacctaatac attcgtagaa acgaatataa aaaatgcaga
     2101 aagagaacag atggaaacat ttttacttta gattgttttt gtcctctttc ggataagggc
     2161 agtgacgaca gccgttatta caacagcagc cgcgtttaag caaaaagcgg gcggttaaaa
     2221 ccattaagcc gtcttcgaaa taataatcga cgccttcgac cggcttttcc tgtttagttg
     2281 tttgttttgt tttcgtcaag ctcatacaaa gccaaaaatt ctttatttcc gtctgcgccc
     2341 agaatcggcg aatcaaccac gccgcaaact ttcaagccgt tttgcgtcgc ccgattgttt
     2401 acttcattaa ttactctttc gtgtttttcc ggctcccgca cgatgccgcc tttgccgacc
     2461 tcgccgcgac cgacttcaaa ctgcggtttt attaaagtga ttattttccc gctgtttttt
     2521 agaagcggcg gcaaagccgg aaaaatcttc gtgacggaga taaacgaaac atccatcacg
     2581 attaagtcaa attttccgtc gaaatcttca ggtttcagat accgcgcgtt cgtgttctcg
     2641 cggacttcga cgcgcgcgtc atttctaagt tgccaaacca tctgatttgt tccgacatcg
     2701 accgtgacca cctttttcgc gccgttctgc aaaaggcaat cggtaaagcc gccggttgag
     2761 gctccgatgt cgagacaaat atattcgttt acggtaatat gaaagtcctg taaggctttt
     2821 tccagtttca agccgccgcg accgacgtat tttgattcgg ctgttttgcc ttttattctt
     2881 attttcgagc cggcggcaaa ttcctgcgaa gatttttcga cgcggcattc gtcgaccaga
     2941 acgacgcccg acatcaccag cgcctgcgct ttcgtccgcg aatcggcaaa gccttgttcg
     3001 accagcaatt tatcaattct ttctttcttc atccggaaat ttttgcatac gactcgcgcg
     3061 cttttatctt tacttcccaa cgcttcccgc tacaatctgc tttaactgaa acaagtaaaa
     3121 aatgttcgaa ttccattaag cgaatctaat tttgcaggtc atttatcttt caaattcaaa
     3181 gtgttatgct aattttgaat ttaccagaat gaaaagggaa acaaaactgt cgagctttgc
     3241 caaatacgcc tggtttgtcc tggcatacaa tgtcgtcgtc atactttggg gcgtttttct
     3301 gcgcgcttcg aaatcgggcg acggctgcgg gcagcattgg ctgacgtgtc acggcgaagt
     3361 ccttccgtcg gctcctcagt taaaaaccgt aattgaattt tcgcaccggc tgacgagcgc
     3421 gctggacgga atttttctca ttattttagt cgtctgggcg tttttgattt ggcgttctaa
     3481 taaatcggaa aacgggcatt tgttaaaagc cgcggtcggt tcgctggtgt ttgtaattat
     3541 cgaaggtctt atcggcgcgg gacttgtcct gacgggaaat acggcggaaa ctttaaccct
     3601 cgcgcgcccg ttctggatgg cggggcattt aataaataca cttattttat taacttttct
     3661 gactttaacg gcttggtttg ccgtcggcgg aaaacccttt aattttaagg cgcagccgaa
     3721 agtcctgttg tttttgggaa tcgccgttct gggaattttc ctggtcggaa tgagcggctc
     3781 ggtcgcggct ctgagcagta tgctgtttcc gtccgaaact ttgaccgaag gaatgacgaa
     3841 agatttttct gccgcgtcgc acattctgct gcggctgcgc atcagccatc cgattttgtc
     3901 gattatggtc gcggtttttc tggtgtttct ggcgggctgg ctcaaatcga gagcgatggg
     3961 aaattaccgt ataaatcgct gggcaaatgt tctgacggtt ctgatcgtca ttcaaattgc
     4021 gtttggcgca ttaacgcttt tgacgctcgc gccgattgtt atgcagattg gacatctgtt
     4081 tttagcggat gcggtttgga ttgttttcgt tctgctgtca gccagctttc tggcggagca
     4141 gaacgaactg ccggaagttt cgtaacggca aaacctcagg aagcagtttc gtaaaaacat
     4201 ttgcgccgca aacgcaaaga ataaactgta atttaaagaa aagagaagat aagattttat
     4261 tttctctttt ttaaattaat ttatcctttg cgcagttcat ccatatttcg tctgcctttg
     4321 cggtcggaac gacgggttta ctgcaaaaca taaaacccag aacgagcagt acggctacta
     4381 tgatgagcgg cacgagccag ccaaacaggg gattttccga ataatcgtcg ccagccaaat
     4441 cgccggtttc ggaaaaagct gtcgtcgtcg ccgctctgtc gccgagcggt tccataccgg
     4501 ctccgaaagc gcccgcgtcc cggtcgaaag tttcggctgc cgccgtgccg atacggtcaa
     4561 ccgaatcggc tgtgtgcgga accgtatccg cagccgattc ataagtttgc gtatcaattg
     4621 ccggcgtttc caaatctaaa aaagttccgt catccggaca gacggcagca ttatctgcat
     4681 attgtttctt acagttcgga catattttca tgcgattttc ctcaatcaga attctttatt
     4741 tttaaatctt taatcaggtt aatgcaac
//
LOCUS       DASWNE010000223         7450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_289334, whole genome shotgun sequence.
ACCESSION   DASWNE010000223 DASWNE010000000
VERSION     DASWNE010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7450
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289334"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VGB68_04495"
     CDS             complement(<1..192)
                     /locus_tag="VGB68_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY0048522.1"
                     /translation="MSKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITKVMSKHNPKMI
                     VRAFDSIDNAPEEKARGITI"
     gene            complement(241..315)
                     /locus_tag="VGB68_04500"
     tRNA            complement(241..315)
                     /locus_tag="VGB68_04500"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(281..283),aa:Thr,seq:ggt)
     gene            complement(349..421)
                     /locus_tag="VGB68_04505"
     tRNA            complement(349..421)
                     /locus_tag="VGB68_04505"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(386..388),aa:Gly,seq:tcc)
     gene            complement(499..586)
                     /locus_tag="VGB68_04510"
     tRNA            complement(499..586)
                     /locus_tag="VGB68_04510"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(550..552),aa:Tyr,seq:gta)
     gene            complement(699..1655)
                     /gene="xerD"
                     /locus_tag="VGB68_04515"
     CDS             complement(699..1655)
                     /gene="xerD"
                     /locus_tag="VGB68_04515"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEY0048523.1"
                     /translation="MPKTPESFRHKTFTMTFEASKKRDLVREYLSYLQVEKGLAKNSL
                     ASYARDLAKLKTWSEKNNFDVIDLSRQDLREFLRDLSRENLSPNSVGRIISTLRGFYK
                     FLLFDRHIAKNPAEDLDAPQKGFYLPKFLNRTEIEELLLVPDVSGEIGLRDRAILETM
                     YAAGLRVSEVCSLRLSDIELDAGILTCKGKGGKTRKVPIGKSAVEWLKSYLVLRRRKE
                     NIEIQNLFVSSLGNPVSRQDVFKMVKECGEKIGREDISPHTLRHSFATHLIQNRADVR
                     SVQQMLGHADISTTQIYTHITDSHLRKTYERFHPRAKNKDKT"
     gene            complement(1661..3346)
                     /locus_tag="VGB68_04520"
     CDS             complement(1661..3346)
                     /locus_tag="VGB68_04520"
                     /inference="COORDINATES: protein motif:HMM:NF025067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HEY0048524.1"
                     /translation="MNERTKTGLEIIEAAVLLGILGDAFLRATPWGLNVLLFTGALAA
                     TMTALVLRRKKEFWNLQTASLHGALIFFAAMFVWRDSVELKFLNILAMLTILVVLTLP
                     ALKIKTQVAGVIHYIYAACLSAISAGFAPFYLLMEDIQWKTIPRAGWTKHLFAVFRGL
                     AIAAPILFVFGGLFMAADAVFQGIVEKTLRINPDILFTHLLIVGFVSWCSAGYLRSSL
                     FSLSSVEDIREETQNEIKPQALSVTAEKTEEAKESAPKDEAPKDEAQKPKEDWDWREM
                     NNSALPKSFTLGAIETGIILGLTNLLFLSFVIVQIPYLFGGMELVQNTPDFKLAEYAR
                     RGFGELVTVAALVLPILLLSHWLLRKDKAVNEKLYRVLAAIQIVLLFVIMISAAQRML
                     LLTGNLGYGLTSVRLYPMAFMIWLALVFIWFSLTVLRAAREQFAWGALWLALFMVGIL
                     HVFNPDEFIVRTNVRLMQQGRSFDSLYVTNLSDDAIPALLDAMPALKFEQQCPVKYKL
                     AQRFREARDENDFRTWNFSRWLARQEMAQYSERLETVGCPVSAQSFSDENSGD"
     gene            complement(3343..4566)
                     /locus_tag="VGB68_04525"
     CDS             complement(3343..4566)
                     /locus_tag="VGB68_04525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0048525.1"
                     /translation="MKSFDVIIVGSGLAGLQCAKLLSQRGAKILLVDRKTDLTKGIHT
                     TGIFVRKTLEDFEFPPETLGKPVRDVTLYSPRLKALNLSSRYDEFRVGKMGLLYESYL
                     RECVKNGVEFLSSTRYVSAERISPERGNQPILKNRIQPIETIVNLEKNGEAFAVKTKV
                     LVGADGANSRVAKDLDLDENREFIVGVEEVWEDVSLTGEPVLHCFLDSRLAPGYLAWV
                     TNDGEEVHIGVGGYHAGFNPREALEEFKAKVIGKIVDLEGAKLAEKRGGKIPVGGVLK
                     RIACECGLLIGDASGAVSPLTAGGLDPCLRLSKMAAQVIWERLETGNPKVLLKFSGEL
                     FRARFVSRLWMRRILGMFSNRFLLEAGCFFLRLPLLKKLAAHVFFGRGSFPDVDLSKS
                     ENNFGRKHKLKGVYQ"
     gene            complement(4563..4685)
                     /locus_tag="VGB68_04530"
     CDS             complement(4563..4685)
                     /locus_tag="VGB68_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048526.1"
                     /translation="MRELGLGETISQKGFRRRQFQQESTESQRNFDVWALTKIL"
     gene            complement(4692..5078)
                     /locus_tag="VGB68_04535"
     CDS             complement(4692..5078)
                     /locus_tag="VGB68_04535"
                     /inference="COORDINATES: protein motif:HMM:NF024988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEY0048527.1"
                     /translation="MRTKGNLALAKENQPEEAPKPNVIHKAAENVSTGLDKLIHERLR
                     LGIISALAVNEALSFTDLKNLLNTTDGNISVHARKLEEAGYVTCEKSFKGRVPLTEYR
                     ITSEGREALTRYLNHMEALINAMKRS"
     gene            complement(5142..5765)
                     /locus_tag="VGB68_04540"
     CDS             complement(5142..5765)
                     /locus_tag="VGB68_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048528.1"
                     /translation="MAKIQALRKEETEASQPVNIGDRAFDNLRFIRETMERSTHFTAV
                     PGYGGIFMGLTAIAASVIAFNQSSVRDWLLVWLTEAVLAFAIGLFAMWQKSKIAKLPL
                     TSAPARKFALGFLPSLLCGVILTLGLWRFEHFEVMIPVWLLLYGAAVVTGGAFSVKVV
                     PVMGWCFIALGTIAFFLPAGYGNWSMLAGFGVLHVIFGIIIARRFGG"
     gene            complement(5990..6820)
                     /locus_tag="VGB68_04545"
     CDS             complement(5990..6820)
                     /locus_tag="VGB68_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048529.1"
                     /translation="MKIFSAALKALILLFGISFVFSACGNSLENQANASDVSRVASVK
                     AAEQTPEQANSSRENSNSAAPQLPDTQAEILDDRDKTTNSEIGNFDFRNYIYPLPRGW
                     QDDDGKDAVLEDGARRMAEKRIGLNYVTARYGDVTGDGADEAFVILKIVTAGSAIPQI
                     VYVFTWKNEKPALIWHFRTGDRADGGLKNIYAENGRLAVEIYGQDRYILGEVETMKIT
                     GDEEQLCCPTHWTRARYEWNGSEFRMQGKRLTFSAADKNAPPVENKNEIFEKQKKGKS
                     "
     gene            complement(7015..>7450)
                     /locus_tag="VGB68_04550"
     CDS             complement(7015..>7450)
                     /locus_tag="VGB68_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0048530.1"
                     /translation="DIVQLGKREMNSRAYVSRFNFSGSDQQKRVGMLSGGERNRVHLA
                     KMLKSGANVILLDEPTNDLDVNTMRALEEALENFAGCAVVISHDRWFLDRIATHILAF
                     EGDSKVVYFDGNYSEYEADKKRRLGHDADQPHRIKYRSLTRA"
BASE COUNT         1779 a         2067 c         1743 g         1861 t
ORIGIN      
        1 gattgtaatg ccgcgcgctt tttcttccgg cgcattgtcg atcgaatcga acgcccgcac
       61 aatcatcttc ggattatgct tgctcatcac tttcgtgatc gccgccgtca atgtcgtttt
      121 cccgtggtcg acgtgcccga tggtgccgat gtttacgtgc ggcttgcttc tgtcaaattt
      181 ctctttgctc atcttttact tatcctcttg aacgattaat aaactgataa aataactgtc
      241 tggagcccaa gacgaggatt gaactcgtga cctcatcctt accaaggatg cgctctacca
      301 ctgagctact tgggctgaat tcttttaatt cagactaaaa tattttgaag cgggagacga
      361 ggctcgaact cgctaccctc agcttggaag gctgatgctc taccaaatga gctactcccg
      421 cccactttaa aggggaaagg ctgaaggata aaggctaaat tttcgccttt atcccttatc
      481 ctttatcctt tagaaaactg gtgcaagggg taggatttga acctacgtag gatattactc
      541 cgccgggttt acagcccggt gccattaacc gctcgaccac ccttgcaatt atttaatgtt
      601 tgtcgagtct tgtttgccag acatttgagt caatagcaaa atcaacatat taacgaagat
      661 ttcccgaagt tgcaagtatg agatgcgtct tttctttttt aagttttatc cttgtttttc
      721 gcacgcggat gaaatcgttc gtaagttttt cgcaggtggc tgtcggtaat gtgcgtgtaa
      781 atctgcgtcg tcgaaatatc ggcgtgtccg agcatttgct gaaccgagcg gacatcggcg
      841 cgattttgaa tcaggtgcgt ggcaaagctg tgacgaagcg tgtgcggcga aatgtcttcg
      901 cgaccgattt tttcgccgca ttccttgacc atcttaaaaa cgtcctgccg cgaaaccgga
      961 tttcccagac tcgaaacgaa caaattctga atctcgatgt tttcctttct gcgccgcaaa
     1021 accagatagc ttttcagcca ttcgaccgcg cttttgccaa tcgggacttt gcgcgttttg
     1081 ccgccctttc ccttgcaggt caaaataccg gcgtccagct cgatgtcgct caggcgcagc
     1141 gagcagactt ccgacacgcg caagcccgcc gcatacatcg tttccagaat cgcgcggtcg
     1201 cgcaagccga tttcgccgga gacgtccggc acgagaagca gttcttcgat ttccgtccgg
     1261 tttagaaatt tcggcagata aaagcctttc tgcggcgcgt ccaaatcttc cgccggattt
     1321 ttggcgatat gccggtcgaa caataaaaat ttataaaatc cgcgcagcgt gctgatgatt
     1381 cgaccgacgg aattcggcga cagattttcg cgcgacaagt ccctcaaaaa ttcgcgcaaa
     1441 tcctggcggc tcaaatcgat aacgtcgaaa ttgttttttt ccgaccacgt tttcagcttc
     1501 gccaaatctc tggcgtagct cgcgagcgaa tttttcgcca agcctttttc gacctgcaaa
     1561 tatgaaagat attcacgaac gaggtcgcgt tttttcgacg cttcaaaagt cattgtaaaa
     1621 gttttgtgac gaaacgattc cggcgttttc ggcaatcaat ctaatcaccg gaattttcat
     1681 cggagaagct ttgcgcggaa accgggcagc cgacggtttc caacctttcg ctgtattgtg
     1741 ccatttcctg ccgcgcaagc caacgcgaaa agttccacgt ccgaaaatcg ttttcgtccc
     1801 gcgcctctct aaatcgctgc gccagtttat atttgaccgg acattgctgt tcgaatttca
     1861 aggcgggcat cgcgtccaga agcgccggga ttgcgtcgtc gctcagattc gtcacgtata
     1921 gcgaatcgaa gctgcgcccc tgctgcatca gccgcacgtt cgtgcggacg ataaattcgt
     1981 ccgggttaaa aacgtgtaga attccgacca taaacagcgc cagccacagc gcgccccacg
     2041 caaactgctc gcgcgcggcg cgcaaaaccg tcaggctgaa ccagatgaaa accagcgcca
     2101 gccaaatcat aaacgccatc gggtaaagcc gcaccgacgt caaaccgtaa ccgagattgc
     2161 ccgtcagcag aagcatcctt tgcgccgcgg aaatcattat gacaaacagc aaaacaatct
     2221 gaatcgccgc cagaacgcgg taaagttttt cattaacggc tttgtctttt cgcaaaagcc
     2281 agtgcgacaa aagcagaatc ggcaaaacca gcgccgccac cgtcaccagc tcgccgaaac
     2341 cgcgccgcgc atattccgcc agcttgaaat cgggcgtgtt ctgcaccagc tccattccgc
     2401 cgaaaagata cggaatctgc acgatgacaa agctcaaaaa caaaagattc gtcaagccga
     2461 gaatgattcc cgtctcgatt gcgccgagcg taaacgattt cggcaaagcc gaattattca
     2521 tctctcgcca atcccaatct tctttcggtt tctgcgcttc gtctttcggc gcttcgtctt
     2581 tcggcgcgga ttcttttgcc tcttccgttt tttccgccgt gacgctcaga gcctgcggct
     2641 ttatttcgtt ttgcgtttct tccctgatgt cttcgacgga agataaactg aacaacgaac
     2701 tgcgcagata gcccgcgctg caccacgaaa cgaagccgac tatcagaaga tgcgtaaaca
     2761 aaatgtccgg gttaatccgc aaagtttttt ccacaatgcc ctgaaaaacc gcgtccgccg
     2821 ccataaaaag cccgccgaaa acgaacaaaa tcggcgcggc gatcgccagc ccgcgaaaaa
     2881 cggcaaacag gtgtttcgtc cagccggcgc gcggaatcgt tttccattgg atgtcttcca
     2941 tcaaaagata aaacggcgcg aatccggcgc ttatcgccga caagcacgcg gcgtaaatgt
     3001 agtgaattac tccggcaacc tgcgttttga ttttcagcgc gggcaacgtc aaaacgacca
     3061 gaatcgtcag cattgccagt atgtttaaaa atttcagctc gaccgaatca cgccagacga
     3121 acatcgccgc aaagaaaatc agcgcgccgt gcaaagatgc ggtttggaga ttccagaatt
     3181 cttttttgcg ccgcagaacc agcgccgtca tcgtcgccgc cagcgcgcct gtaaaaagca
     3241 aaacattcaa gccccacggc gtcgctctta aaaaagcgtc gcccagaatt ccgagtaaaa
     3301 ccgccgcttc gataatctcc aaacccgttt ttgtccgctc gttcattgat aaactccctt
     3361 taatttgtgc tttcgaccaa agttattttc gctttttgat aaatcgacgt ccgggaaaga
     3421 gccgcgcccg aaaaagacgt gcgccgccag ttttttcagc agcggcaggc gcaggaaaaa
     3481 gcagcccgct tccagcagaa accggtttga gaacattcct aaaatccggc gcatccaaag
     3541 ccttgacacg aagcgcgcgc gaaaaagttc cccggaaaat tttaataaaa cctttggatt
     3601 tcccgtttcg agcctttccc aaatcacctg cgccgccatt ttcgataaac gcaggcacgg
     3661 gtcgagcccg cccgccgtga gcggcgaaac cgcgcccgaa gcgtcgccga taagcagccc
     3721 gcactcacac gcgatgcgct ttagaacgcc gccgaccgga atttttccgc cgcgtttttc
     3781 cgcgagtttc gccccttcca aatcgacgat ttttccgata actttcgcct taaactcttc
     3841 taacgcttcg cgcgggttaa agccagcgtg ataaccaccg acgccgatgt ggacttcctc
     3901 accgtcgttc gtcacccagg cgagatagcc gggcgcaagg cgcgagtcga gaaaacaatg
     3961 cagaaccggc tcgcccgtta aggaaacgtc ttcccagacc tcttcgacgc cgacgatgaa
     4021 ttcgcgattt tcgtccaaat ccaaatcttt cgccacgcgc gaattcgcgc cgtccgcgcc
     4081 gactaaaact tttgttttga cggcaaacgc ttcgccgttt ttttccagat tgacgattgt
     4141 ttcgattggt tgaattcggt tctttaaaat cggctgatta ccgcgttccg gactgattcg
     4201 ttcagccgaa acgtaccggg tcgagctcaa aaactcgacg ccgtttttca cgcattcgcg
     4261 cagatagctt tcgtaaagca agcccatttt tccgacgcga aactcgtcgt atctgctcga
     4321 caaattcagt gccttcaaac gcggcgaata cagcgtcaca tcgcgcacgg gtttgcccag
     4381 cgtctcgggc ggaaactcga aatcttccag cgttttacga acaaaaattc ccgtcgtgtg
     4441 aatgcctttc gtcaaatccg tcttgcggtc gacgagcaaa atttttgcgc ctcgctgcga
     4501 aagcagcttg gcgcattgca gaccggctaa acctgagccg acgattatca cgtcaaaact
     4561 tttcataaaa ttttagtcaa agcccaaaca tcgaaatttc tttgcgattc ggtcgattcc
     4621 tgctgaaact gccgccgccg gaatcctttt tggctgatgg tttctcctaa tccgagttcc
     4681 cgcataaaac gctaactcct tttcatcgca tttatcaatg cttccatatg attcagataa
     4741 cgggtcagcg cctcgcgtcc ttcgctcgta atccggtatt cggtcaacgg cacgcgacct
     4801 ttaaaagatt tttcgcacgt gacgtaaccg gcttcttcca gttttcgcgc gtggacgctg
     4861 atgttgccgt cggtcgtatt caataaattc ttcaaatccg taaaagacaa agcttcattt
     4921 accgccagcg cgctgataat accgagccgc agacgctcgt gaatcagttt gtcgagcccg
     4981 gtcgaaacgt tttccgccgc tttgtgaatc acattcggtt tcggcgcttc ttccggctga
     5041 ttttcctttg ctaaagctaa atttcctttt gttcgcataa ttttataagg cgtattttaa
     5101 ttttaacttc cttgcccgcg ctcgagcgcg ggcttctaca gttagccgcc aaatcttctg
     5161 gcgataataa ttccgaaaat cacgtgcaga acgccgaaac cggcgagcat cgaccagttg
     5221 ccgtatccgg cgggcagaaa aaacgcgatt gtcccgagcg cgataaaaca ccagcccatc
     5281 accggaacga ctttcaccga aaaagcgccg cccgtgacga ccgccgcgcc gtaaagcaga
     5341 agccacaccg gaatcatcac ttcaaaatgt tcgaatcgcc aaagcccgag cgtcaaaata
     5401 acgccgcaca gcaaagacgg cagaaatccg agcgcaaatt ttcgggcggg cgccgacgtc
     5461 agcggcagct tcgcaatttt cgatttctgc cacatcgcaa aaagcccgat ggcgaaagcc
     5521 agaaccgctt ccgtcagcca gactaaaagc caatctctga ctgaagattg attaaaggcg
     5581 atgacgctcg ccgcaatcgc cgtcaagccc ataaaaattc cgccgtaacc gggaaccgcc
     5641 gtgaaatgcg tcgagcgctc catcgtttcg cggataaacc gcagattgtc gaatgcccgg
     5701 tcgccgatat ttaccggctg gctcgcttcg gtttcttctt tgcgcagcgc ctgaatcttc
     5761 gccatatgtt ttgaaattta tattaagttg ttttatttgt caagaacttt atattacaaa
     5821 gcaacaaaaa aaactttgaa agcggtaaac ggtaaacggt aagtggtaag ttgcagtctc
     5881 aaaatgtcga aatttttatt taaactcgat attcagaaca acgaacttac cacttaccgt
     5941 ttaccgttta ccactgattt accgtttacc gcttaccgct gataagcttt cagcttttcc
     6001 cttttttttg tttttcgaaa atctcgtttt tgttttccac cggcggcgcg tttttatccg
     6061 ctgcggaaaa agtcagacgc ttgccctgca tccgaaactc gcttccgttc cattcgtagc
     6121 gagctcgcgt ccaatgcgtc gggcagcaaa gctgttcttc gtcgccggtg attttcatcg
     6181 tttcgacctc gcccagaatg tatcggtctt gcccgtaaat ctcgacggcg agccgtccgt
     6241 tttcggcgta aatatttttc aagccgccgt cggcgcggtc gcccgtgcga aaatgccaga
     6301 taagcgcggg cttttcgttt ttccacgtaa aaacatagac gatttgcgga atcgccgagc
     6361 ctgccgtgac gattttcaga atcacgaaag cctcgtcagc gccgtcgccc gtcacgtcgc
     6421 cgtatctggc ggtcacataa tttaaaccga tgcgcttttc cgccatgcgc cgcgcgccgt
     6481 cttccagaac ggcgtccttg ccgtcgtcgt cctgccagcc gcgcggcaga gggtaaatgt
     6541 aatttctaaa atcgaaattg ccgatttcag aattcgtggt tttgtcgcgg tcgtcgagaa
     6601 tttcggcttg cgtgtcgggc agctgcggag cggcggaatt tgaattttcc cggcttgaat
     6661 tcgcctgctc cggcgtttgt tcagccgcct ttacggaagc aacgcgcgaa acgtcagacg
     6721 catttgcctg attttcgagg ctgtttccgc aagcggaaaa aacaaatgaa atgccgaaaa
     6781 gcagaattaa tgctttcaaa gcggcggaaa aaattttcat ttagattcgg ggaaagccca
     6841 agacggatag aaacttgcgg caatagatta gcacgaatcg cgtttttgta aaaatattcg
     6901 tgcgaatcgt tttttaaagc cggttcggga caagaaagaa tcataaggct acaggcgctc
     6961 gcttgtgatt ccgtttctct tatcccgaac cggctttgtt tctgtgtttt gcggttaagc
     7021 ccgcgtcaga ctgcggtatt tgatgcggtg cggctggtcg gcgtcgtgtc cgagacgacg
     7081 ttttttgtcg gcttcgtatt ccgagtaatt gccgtcgaaa tagacgactt tcgaatcgcc
     7141 ttcaaacgcc agaatgtgcg tcgcgatgcg gtcgagaaac caacggtcgt ggctgatgac
     7201 gacggcgcag ccggcaaaat tttccagcgc ttcttcgagc gcgcgcatcg tgttgacgtc
     7261 caaatcgttg gtcggctcgt ccagtaaaat gacgtttgcg cccgacttga gcattttcgc
     7321 cagatgcacg cggtttctct cgccgccgga aagcatcccg acgcgcttct gctggtcgga
     7381 acccgaaaaa ttgaagcgcg agacgtaagc gcgcgaattc atctcgcgct tgccgagctg
     7441 aacgatgtcc
//
LOCUS       DASWNE010000224         7072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_393778, whole genome shotgun sequence.
ACCESSION   DASWNE010000224 DASWNE010000000
VERSION     DASWNE010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7072
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393778"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1884
                     /locus_tag="VGB68_04555"
     CDS             <1..1884
                     /locus_tag="VGB68_04555"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0048531.1"
                     /translation="QGVTRLDILNYISHGISKNDNLFFNDLETGIEDEDAEFAEERAG
                     GKRPKPLESFTVELVALAKTGGIDPIIGRLTEIERTIQVLCRRKKNNPLYVGEPGVGK
                     TALAEGLALKISEGDVPDVLKDAKVFALDMGAVLAGTRYRGDFEQRFKAIINDLKKHE
                     NSILFIDEIHTIVGAGAVSGGAMDASNILKPALASGELRCIGSTTYSEYKAAFERDRA
                     LARRFQKIEIGEPTIDETYQILKGLKRFYEEHHNVQYTDAALKTASELAGKYINDRFL
                     PDKAIDVIDEVGAATKLLPEDKRPKRISVSMVENTIARMAKIPPKTVVADEKDRLKTL
                     REDLKNVIFGQDEAINKVVDAIQISRAGLGHESKPVGSFLFSGPTGVGKTELSKQLAE
                     QLGIEFLRYDMSEYAEPHTVSRLIGAPPGYVGFDQGGLLTEAIMRTPHAVLVLDEIEK
                     AHPNLFNLLLQVMDSATLTDNNGKKADFRNVILIMTTNAGARELSSSGVGFRNQTETK
                     GQAKGAIERTFTPEFRNRLDAWVPFRALDLDIIKLIVDKFIKELNGQLMEKRVLVQLD
                     EAAREWLAKNGFDARYGARPMSRLIHEKIKQPLANEILFGKLTNGGSVSISETGGELI
                     LNF"
     gene            2068..4941
                     /locus_tag="VGB68_04560"
     CDS             2068..4941
                     /locus_tag="VGB68_04560"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0048532.1"
                     /translation="MRRLRILLFALFLSAMNVFGQQTNGTIRGRVVDVNDAVIVGANV
                     TVTGADGVERKAQTNQSGEFNITLAPGKYTVRVASPGFAAYENTEVSAAAGRVVALDV
                     KLGVTIEETQVTVGEEAAINTDPETNASAIVLKASDIEVLPDNEAELEAALRALAGPS
                     TGPSGGEIFIDGFSGGRLPPRDTIREVRINQNPFSSEYDRLGFGRIEILTKPGTEKLR
                     GELEFEFEDESLNSRNPFAPNRAPFQVRTVEGNLGGPIIKNRASFFVDFGREETDDNA
                     LVNTLILDPNLNITPFQLAVRAPSKDIEFSPRLDIQLNRNNTLVARYFFERTDSENAG
                     LGGFDLLSRGFCESDSEHTLRLTETAVISGSIINEARFQYIRRRTSQESADNSPTIRV
                     NDAFTGGGAGFGFAFADADRFELQNYTSFVRGRHSLKIGGRLRHFRLVNSSPVNFAGT
                     FTFTTLEQYRDTILNLPGASPSQFSIAGGNPQAGVAQTDLGLFFQDDWRVRPDLTLSF
                     GLRYENQNNISSNLNFAPRFGFAYAPGASGQNKPKTVFRGGFGIFYDRFGESLTLQAI
                     RFNGVNQQQFIVTDPAILDAVIFTRNGVSNVPTVSRLAAFAQPQTTRVVAPDLQAPYT
                     IQSAAGVERQLPFKTSFSATFVSAQTRRLLRSRNINAPIDGVRPNSAGGNIFQYESSG
                     RFNQSQLIFNLRSNFIDGISIFANYAFGKAKSDSDGAATFPANSYDLRGEYGDALLDI
                     RHRFVIGGNFEAPFGINLSPFITFRSGAPFNITTGTDANGDALFNDRPAFAADLNRQC
                     NFGTATNPSIRDCVVQTEFGSFDLQPIAGQTIIPRNYGRGSEFFVVNLRATKEFGFGG
                     EKKGASNRGQGGGGNRGGINNPLGVGGGQRGGGDNESKYKLELSMQIRNLFNRTNGGT
                     PVGNLRSEFFGQPVSLAGGFGFGGGGSQAAGNRRIEFEVEFSF"
     gene            5038..5457
                     /locus_tag="VGB68_04565"
     CDS             5038..5457
                     /locus_tag="VGB68_04565"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY0048533.1"
                     /translation="MKEFGEKVKGVEYLQRAGSYGVIIKDGRAGVLKASGYDTYFLVG
                     GGIEENESETEALRREAAEEIGFHIEVGEKIAEAIEYFYSAGEEKYVAKECRFYRASL
                     MDKTVEKGKHQLVWITTNELEEMHHQSYRWILEKELK"
     gene            complement(5566..6918)
                     /locus_tag="VGB68_04570"
     CDS             complement(5566..6918)
                     /locus_tag="VGB68_04570"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY0048534.1"
                     /translation="MLFAICVRADEVDRYVRAQLAERHIPGAAIAVVKNGRVVKTEGY
                     GLASVEFSAPVTKETVFEIGSVSKQITAAAIMLLVEKGKINLDEKISKYLPATPDAWK
                     NVSVRNLLTHTSGIKSYTGLSGFELTKRLSRDEFIKALAAQPLEFETGSRYLYSNSGY
                     NLLGFIVETVSGESYWDFTRERIFKPLGMNSTADRDPKYIIPNRASGYEWQNNALVGR
                     DYDLTDVFSAGAIVSTVEDLVKWEAALRGDALLKKESKTQMWTPVTLNDGKPYPYGFG
                     FRLSQIRGHKIVGHSGQTAGFGANISRYTDDDLTVIVLTNLGEIGMGTLLANGIAKIY
                     IPSISLKALKAQTEPDAKISKTISTALRERLENKLNPDYLTEELIKSLSTERAKSNIQ
                     RINSFGAIKNPVFVGDETSGSGAKIYRYKAETPRRIFLWRFAFNDAGKISEMTLEEEE
                     "
BASE COUNT         1723 a         1827 c         1917 g         1605 t
ORIGIN      
        1 cagggcgtga cgcggctcga tattttaaat tacatctcgc acggcatttc caaaaacgac
       61 aatttatttt tcaacgattt ggaaaccggc atcgaggacg aagacgccga atttgccgaa
      121 gaacgcgccg gcggaaagcg tccgaaaccg ctcgaatctt tcacggtcga gctggtcgcg
      181 ctggcaaaga cgggcggaat cgacccgata atcgggcgct tgacggaaat cgagcgcacg
      241 attcaggttc tctgccgccg caagaaaaac aacccgcttt acgtcggcga gccgggcgtc
      301 ggcaaaacgg cgctggcgga aggcttagcc ttaaaaatca gcgaaggaga cgtgccggac
      361 gttttaaagg acgcgaaagt ctttgcgctc gatatgggcg cggtgctcgc cggaacgcgt
      421 taccggggcg atttcgaaca acgtttcaaa gccattatca acgaccttaa aaaacacgaa
      481 aactcgattc tttttatcga cgagattcac acgattgtcg gcgcgggcgc cgtctccggc
      541 ggcgcgatgg acgcttccaa tatcttgaaa ccggcgctgg cgtcgggcga actgcgctgt
      601 atcggctcga cgacctacag cgaatacaaa gccgcgtttg aacgcgacag agctttagcc
      661 agacgttttc aaaaaatcga aatcggcgag ccgacgattg acgaaactta tcagatttta
      721 aaaggcttaa agcgatttta cgaagagcat cacaatgttc aatacacaga cgcggcgctg
      781 aaaaccgctt cggagctcgc cggaaaatac atcaacgacc gttttttgcc cgataaggcg
      841 attgacgtca tcgacgaggt cggcgcggcg actaagcttc ttcccgaaga caaacgcccg
      901 aaacggattt ccgtctcgat ggtcgaaaac acgattgcgc gaatggcgaa aattccgccg
      961 aaaacggttg tcgccgacga aaaagaccgc ttgaaaaccc tgcgcgagga tttgaaaaac
     1021 gtcattttcg ggcaggacga agcgattaac aaagtcgtcg acgcgataca gatttcgcgc
     1081 gccgggctcg gtcacgaatc gaaaccggtc ggctcgttcc tgttttcggg tccgaccggc
     1141 gtcggcaaaa ccgaactttc aaaacagctt gccgaacagc tcggcatcga atttctgcgc
     1201 tacgatatga gcgaatacgc cgagccgcac acggtttcgc gtctgatcgg cgcgccgccg
     1261 ggatatgtcg gctttgacca gggcgggctt ctgaccgaag cgattatgcg cacgccgcac
     1321 gccgttctcg tgttggacga aatcgaaaaa gcgcacccga atcttttcaa cctgctcctt
     1381 caggtaatgg attcggcgac cttaacggac aacaacggca aaaaagccga ttttcgcaac
     1441 gtgattttga ttatgacgac caacgcgggc gcgcgcgaat tgtcttcgag cggcgtcggc
     1501 ttccgcaatc aaaccgaaac gaaaggacag gcaaaaggcg ctatcgagcg cactttcacg
     1561 ccggaattcc gcaatcgtct cgacgcgtgg gttccgttca gggcgctgga cctggacatc
     1621 atcaagctga ttgtggacaa attcatcaag gagctgaacg gtcagttgat ggaaaaacgc
     1681 gttctcgtcc aactggacga agcggcgcgc gaatggctgg caaaaaacgg cttcgacgcg
     1741 cgttacggcg ctcgcccgat gtcgcgcctg attcatgaaa aaatcaagca gccgctcgcc
     1801 aatgagattc tattcggtaa attaacgaac ggcggcagcg tttcgatttc ggagacgggc
     1861 ggcgagttga ttttgaattt ctgaaaggat gacgtttaaa atccatatat atttatgact
     1921 taaaaataag tgaggtgttt acatctcgct tatttttttc ctttccacta accggacggc
     1981 tgtctcaaac gagcaatttt tctggaaggc tggctgacgt ttatgctagt tttctaaata
     2041 aggcacttat tttagcaagg ggtttttatg agacgattga ggattttgct tttcgcgctc
     2101 tttctgtcgg caatgaatgt tttcggacag caaacaaacg gaacgattcg cgggcgcgtc
     2161 gtcgatgtca atgacgccgt tattgtcggc gcaaacgtta ctgtcaccgg cgcggacggc
     2221 gtcgagagaa aagcgcaaac caatcaatcg ggtgaattta atattactct cgcgcccgga
     2281 aaatacacgg ttcgcgtcgc tagtccgggc tttgccgctt acgaaaacac ggaggtttcg
     2341 gctgccgccg ggcgcgtcgt ggcgcttgac gtcaagctcg gcgtgacgat tgaggaaact
     2401 caggtaacgg tcggtgagga agcggcgatt aataccgacc cggaaacaaa cgccagcgcg
     2461 attgttttaa aagcatcgga catcgaagtt ctgccggaca acgaagccga actggaagcc
     2521 gctttgcgcg ctctcgccgg accttcgacc ggaccgagcg gcggcgagat ttttatcgac
     2581 ggattttcgg gcgggcgact tccgccgcgc gataccatca gagaggttcg catcaatcag
     2641 aacccgtttt cttccgagta tgaccggctg ggcttcgggc gcatcgagat tctgaccaag
     2701 cccggcacgg agaaactgcg cggcgagctt gaatttgaat tcgaagacga atccttaaac
     2761 tcgcgcaatc ctttcgcgcc gaatcgcgcg ccgtttcaag tgcgaaccgt cgagggcaat
     2821 ttaggcggac cgattatcaa aaaccgcgct tcttttttcg tcgatttcgg acgcgaggag
     2881 actgacgata acgcgctcgt caatacgctg attctcgacc cgaacttaaa catcacgccg
     2941 tttcagctcg ccgtgcgcgc gccttcgaaa gacatcgaat tcagcccgcg cctcgacatt
     3001 cagcttaacc ggaacaatac gctcgtcgcg cgttactttt tcgaacgcac ggactcggaa
     3061 aacgcgggct tgggcggttt cgatttgctt tcacgcggct tttgcgaaag cgattcggaa
     3121 cacactctgc gattgaccga gacggcggtg atttccggct caatcatcaa cgaagcgcgt
     3181 tttcaataca ttcgccgccg cacatcgcag gaaagcgccg ataattcgcc gacgattcgc
     3241 gtcaacgacg cttttacggg cggcggcgcc ggtttcggat ttgcgtttgc cgacgccgac
     3301 cgtttcgagc tgcaaaatta cacttctttc gttcgcggca gacactcgct caaaatcggc
     3361 gggcggctgc ggcactttcg ccttgttaat tcttcgccgg ttaatttcgc cgggactttt
     3421 acatttacaa ctctcgaaca atatcgagac acgattctta atttgccggg cgcaagccct
     3481 tcgcagtttt ccattgcggg cggaaatccg caagccggcg tcgcgcagac ggatttgggt
     3541 ttgttttttc aggacgattg gcgcgtgcga cccgatttga cgctttcgtt cgggctgcgt
     3601 tatgaaaacc agaacaacat ctccagcaat ctcaactttg cgccccgttt cggcttcgcc
     3661 tacgcgcccg gcgcgagcgg gcagaataag ccgaaaacgg tttttcgcgg cggcttcggg
     3721 attttttacg accgtttcgg cgaaagcctg acgcttcagg caatccgctt taacggcgtc
     3781 aatcaacagc agtttatcgt caccgacccg gcgattctgg acgcggtgat ttttactcgg
     3841 aacggagttt caaacgtgcc gaccgtcagc cggctcgcgg ctttcgcgca gccgcagaca
     3901 acgcgtgtcg tcgcgccgga tttgcaagcg ccttatacga ttcaaagcgc cgccggcgtc
     3961 gagcgtcaat tgccttttaa aacaagcttc agcgcgactt tcgtcagcgc gcaaacgcgc
     4021 cgcctgttgc gctcgcgcaa catcaacgcg ccgattgacg gcgtcagacc gaattctgcg
     4081 gggggcaata tttttcaata cgaatcgagc ggacgcttta atcaaagtca actgattttt
     4141 aacctgcgct cgaattttat cgacggcatt tcgattttcg ccaactacgc tttcggcaaa
     4201 gcgaaaagcg attcggacgg cgcggcaact tttccggcga attcttacga tttgcgcggc
     4261 gaatacggcg acgctctgct cgacatccgg catcgctttg tcatcggcgg caattttgaa
     4321 gcgccgttcg gaataaatct gagtccgttc atcacgtttc gctccggcgc gccgttcaat
     4381 atcacgaccg gcacggacgc caacggcgac gcgcttttca acgaccgtcc ggcgttcgcc
     4441 gccgatttaa accggcaatg caatttcggg acggcgacaa atccgtcgat acgcgactgc
     4501 gtcgtgcaaa ccgaattcgg cagcttcgat ttgcagccca ttgccggtca gacaattatt
     4561 ccgcgcaatt acggcagagg ctccgaattt ttcgtcgtca acctgcgcgc gaccaaagag
     4621 ttcggctttg gcggcgagaa aaagggtgcg tcaaatcgag gacagggcgg cggcggaaat
     4681 cgcggcggaa ttaacaaccc gctcggcgtc ggcggcggac aacgcggcgg cggcgacaat
     4741 gaaagcaaat ataagctgga gctttcgatg cagattcgca atctttttaa tcgcacgaac
     4801 ggcgggacgc ccgtcggaaa tttgcgctcg gaatttttcg ggcagccggt ttcgctcgcc
     4861 ggaggtttcg gcttcggcgg cggcggcagc caggcagcag gcaatcgccg catcgaattt
     4921 gaagtggaat ttagttttta aaaaaaaagt cggcgcgctt attaaaagcg aaaatttaat
     4981 cgaaagcggc gttaaaacca aacgcggctt tcgattccgg aaaggggaat aaaagcaatg
     5041 aaagaattcg gcgaaaaagt aaaaggcgtt gaatacctgc aaagagcggg aagctacggc
     5101 gtgataataa aagacggtcg agcgggcgtt ttaaaggcga gcggctacga cacgtatttt
     5161 ctggtcggcg gcggaatcga agagaacgaa agcgaaacgg aagcgctgcg gcgcgaagcg
     5221 gcggaagaaa tcggttttca catcgaagtc ggagaaaaaa tagctgaggc gattgagtat
     5281 ttttattccg ccggagaaga gaaatacgtc gccaaagaat gccgctttta tcgcgcctcg
     5341 ttaatggata aaacggtcga gaaaggcaag catcaattag tttggattac aacgaatgag
     5401 cttgaggaaa tgcatcatca aagctatcga tggattcttg aaaaggaatt aaaatgaata
     5461 ttataaattg aagaaaacaa gtcggctgca aatcgtaatt aacgccgaac gcagagcgcc
     5521 gaatgaagga aatatttaat ttagcgcttt gcgttcggca tttgattact cttcttcttc
     5581 aagcgtcatt tccgagattt tgcccgcgtc gttaaacgcg aaacgccaga ggaaaatgcg
     5641 gcgcggcgtt tcggctttgt agcgatagat tttcgcgccg ctgccggaag tttcatcgcc
     5701 gacgaaaacc ggatttttga ttgcgccgaa agaattgatg cgttgaatgt tggatttcgc
     5761 gcgttcggtg gaaagcgatt ttatcagctc ttcggtcaaa taatcgggat ttaatttatt
     5821 ttccaatcgc tcgcgcagcg ccgtggaaat cgttttggaa attttcgcgt ccggttcggt
     5881 ttgcgccttt aaggctttca gcgaaatcga cggaatataa attttggcga tgccgttcgc
     5941 gagcagcgtg cccattccga tttcgccgag attcgtcaag acaatcacgg tcaaatcgtc
     6001 gtccgtatag cgcgagatgt tcgcgccgaa accggcggtc tgcccgctgt gcccgacgat
     6061 tttatgccct cgaatctgcg aaagccggaa gccgaaaccg tatggataag gctttccgtc
     6121 gtttaatgtc acgggtgtcc acatctgcgt tttgctttcc tttttcagca gcgcgtcgcc
     6181 ccgcagagcg gcttcccatt ttaccaaatc ttcgacggtc gaaacgattg cgcccgcgct
     6241 gaaaacgtcg gtcaaatcgt aatcgcgccc gaccagcgcg ttgttttgcc attcgtatcc
     6301 gctcgcgcga ttcgggatta tatatttcgg gtcgcggtcg gcggtcgagt tcattccgag
     6361 cggtttgaaa attctttccc ttgtaaaatc ccagtaagat tcgcccgaaa ccgtctcgac
     6421 gataaaaccg agaagattgt agccgctgtt gctgtagaga tagcgcgagc cggtttcaaa
     6481 ttcgagcggc tgcgcggcga gcgccttgat aaattcgtcg cggctcaatc ttttcgtaag
     6541 ctcaaagccg gacaaacccg tgtagctttt aatgccggaa gtatgcgtca aaagattacg
     6601 aacgctgacg tttttccacg catcgggcgt ggcgggcaga tatttcgaga ttttttcgtc
     6661 cagatttatt ttgccctttt cgaccagaag cataatcgcc gcggcggtaa tctgcttgga
     6721 aaccgagccg atttcaaaga cggtttcttt ggtcacgggc gcgctgaact cgacgctcgc
     6781 cagcccgtag ccttcggttt tgacgacgcg accgtttttg acgacggcaa tcgccgcgcc
     6841 gggaatatgt cgctcggcga gctgcgcgcg gacgtatcta tcgacctcgt cggcgcgaac
     6901 gcaaattgca aaaagcaaaa aaacaaaaat gaaaaataaa gaaaagcccg ggcgcttgaa
     6961 tctggaattc atagatttta tagaacaata gaaattgtaa gggattttta cgcggaaact
     7021 ttcggcaagt caagaacgaa ttgtccggca ggcggcaagt tataaaaaat ac
//
LOCUS       DASWNE010000225         8423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_6943, whole genome shotgun sequence.
ACCESSION   DASWNE010000225 DASWNE010000000
VERSION     DASWNE010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8423
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6943"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            456..1232
                     /locus_tag="VGB68_04575"
     CDS             456..1232
                     /locus_tag="VGB68_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048535.1"
                     /translation="MNNVPRQMLCRILAKHGKEICTDARRCEGLLNDLCGSYRREINV
                     LVNALEERIPLDLMAASSSMPLELLLTRLEKRLEDQLGLTAEAARWAVESWALALNVV
                     TEIEIQQRLRKRVDSDERLSETEKIQPKDSNSGKTSNLNRSAPPAAPLPPPNAQRTIP
                     EPQAKPTPARNQPGKMPVPAPPVLIPANNQPANQPQIQTPNPALPRKRSFSIIRGCLI
                     MFFLLIISTVLLFFGAPYAIKVMRETQQERDSESPRFPSR"
     gene            1409..2227
                     /locus_tag="VGB68_04580"
     CDS             1409..2227
                     /locus_tag="VGB68_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0048536.1"
                     /translation="MIKRPGGELASRPLHFIWIADSSGSMEQDGKIQSLNTAIREAIP
                     HMKKVAEDNPNAQVLVRAVKFSNGAQWHISQPTPVADFAWTDLVAEGETDMGKALELV
                     SEQLKMPPMSDRALPPVLVLVSDGQPTDDFDAGLKALMEQPWGKKAVRIAISIGKDAD
                     DEVLQKFIGNSELKPLAANSPEALVKHIKWASTAVLKSASSPAAGAEVPAGTDTANVP
                     IPTAVPIGATEATGTTGTTGATGTTNATAATAPTGTPAGSPVDPNDQNAVDDVW"
     gene            2237..3370
                     /locus_tag="VGB68_04585"
     CDS             2237..3370
                     /locus_tag="VGB68_04585"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HEY0048537.1"
                     /translation="MTENKNAIEWRVIGETVPGASHLRAGVPNQDSLLYVRESSRALP
                     VVLSVSDGHGSPKCFRSDRGSQFAVKKAAYLVSEFLDERRGKSDASEIESQADYLSKE
                     FVRRWREAVEDDLKKEPFTEKEFEALEKKSDAKARRLVEENPLLAYGATSLTVAVEED
                     FILYMQLGDGDILNVSATGEVIKPLAEDPRLLANETTSLCLPKAEKDFRCLVQKISAD
                     ESPAMILLSTDGYLNSFSSETGFFQAGTDILQMLGADGGFDAVNDNLKAWLEEATRMG
                     SGDDCTVAVIYRPDFLKKSEKIEKSKEIETTTIEAPPTPETSQAIAALTDSETVAPEN
                     MEDVTVTITIKKDKNKKAASPAQPDSEQKAHPDEHKNSVKKVS"
     gene            3367..4908
                     /locus_tag="VGB68_04590"
     CDS             3367..4908
                     /locus_tag="VGB68_04590"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HEY0048538.1"
                     /translation="MNELPRRKLIEIVARKGRSIIENPGRVKGLLRDYCGAYRREISV
                     LAMALEEHAVPDLLSAPASLPRKVLLARLARRLCDNLALSEEAAIWSIESWALALSVI
                     SAAELETNETAPAAALHVVKPREEISSTAAAASQNRPANSVQSKPRANSFIVAADGSG
                     DFKTIGEALQKVAPNARLLVREGFYDESIILDKNIEILGDGAFDKIIVRSVNSSCILM
                     QTDKASVRGLSLRGSGARGGKAFFAVDIPKGELILESCDISSDSLSCIAVHGVNSKPA
                     IKNCRIHDGADSGLYFYDNARGRVENCDVYRNANVGVAITQGANPALANCRVFQGNNG
                     GVVAWGNGAAGVIENCHIFGHRLANIGISEYANPIFRRCEIYNGQDTGVYVHANGFGT
                     LEECNIYKNSKAEVGVSQGGNSIFRRCAIHDGENSGVILQNRGRTTLESCNIYDNADA
                     GVAIYGESTVIIRRCNIHRNGKVAVRVKEQSAARVENCDLRGNRLAAWETEYGITLEE
                     SGNRD"
     gene            4937..6259
                     /locus_tag="VGB68_04595"
     CDS             4937..6259
                     /locus_tag="VGB68_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048539.1"
                     /translation="MDQILKPGQVIQCQPSGMPCTIETLLGAGGQGEVYKAELGGEAV
                     AVKWFFPHMATPEQLASMQLLVRKGPPSEHFLYPMEITTAAGVQGFGYIMPLRPKRFK
                     NIVDLMTRRIEPTFRALATAGLNLSHNYLLLHSQGLCYRDISFGNAFFDPDNGDILIA
                     DNDNVSVDGAGVLGVLGTPRFMAPEIVRGEALPSTQTDLFSLSVLIFYMLMVAHPLEG
                     KKEAAIKALDLPAMTKLYGKEPIFIFDPNDDSNAPVPGIHDNALAFWRIYPQFLRDIF
                     IRAFTIGIRDPKNGRVRESEWRGTMVELRDVIFYCPHCSLENFYDSQALKASGGNPGL
                     CWACAAQLRLPPRIRIGKSVVMLNHDTKLFPHHTDDQKLYDFSAPVAAVAVHPTNPNI
                     WGLKNLSGDKWFITAADGSVKDVEPQRSVTLAVGTKINFGKSEGEIRL"
     gene            6711..7382
                     /locus_tag="VGB68_04600"
     CDS             6711..7382
                     /locus_tag="VGB68_04600"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEY0048540.1"
                     /translation="MRILFYLLPLLVIGWTVWGIFAYKELKTALKKGERGALLREYKI
                     TILGEIIGGLTAFGAIGYEVFKSSDTFGISFGEGGNFLIAAAAVGSLLGLIIVPLLAK
                     FGKNVMVGDIEALLPRTKNEKLWFILVALSAGVCEELIFRGFGLRLFESFGFTGIALL
                     IVTAVVFGLVHIYQGFLGVITSAIMGALFTLIYIQTGSLWWAIAAHIFIDLRVLLLVA
                     FAKKH"
     gene            7766..8266
                     /locus_tag="VGB68_04605"
     CDS             7766..8266
                     /locus_tag="VGB68_04605"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEY0048541.1"
                     /translation="MSRGFVKEGDQEEIPILTPRAYLPDGVTNYVTQVGMNELLDEKQ
                     MLMGEIENLDMTNENERRIASNHLNAKLKLLNERIHTAEIIDLEKQSPDKVWFGATVT
                     LKTDNETKLQKYQIVGVDEADVSQGKISFISPIAKILMGKEAGEKAILKLANGNRTYE
                     IIEIVY"
BASE COUNT         2262 a         1964 c         2177 g         2020 t
ORIGIN      
        1 gattggattg tttgcgaaaa agcgcaataa tttgcggcta aagtaaaaat tgcggcgaaa
       61 actacgcttt ttagcgccgc cgtcataatt ttcccgattc tgtaaatttt attgctcata
      121 atgtgtcgat taattccgag aaaaattcat tgaaaattta ttatcaaacg ccggtcaaag
      181 gcgactgccg gataagtaat acgcaaatga agagaattag ttatatcggt ctcatagaac
      241 gaaataaatc aaagtgattc gctcgaaaaa gcgcggcgaa caatatcgga ttttagcctt
      301 gcaatctcga aaaaagacgt ttccgagacc gccgaacgat gttgattcac ctttgcctgt
      361 ttctctgaat tcggtctgct cactttaaca atgaaatctt attcgtattt ttcgatttgc
      421 attgaagatt gtgtagttat tgaataaaat tttgtatgaa taatgtaccg agacaaatgc
      481 tttgccggat tttggctaaa cacggcaagg aaatttgcac tgatgcccgc cgctgcgaag
      541 gtcttttaaa tgatttatgc ggctcttacc ggcgtgaaat aaatgttttg gttaatgcgc
      601 tcgaagagcg tattccattg gatttaatgg cggcgagcag ctcgatgccg ctggaattgc
      661 ttttaacgcg gctcgaaaaa aggctcgaag accagttggg tctgacggct gaagcggcgc
      721 gctgggcagt tgaatcctgg gcgctggcgc ttaatgtagt gaccgagatt gaaattcagc
      781 aaagactgcg aaaacgcgtt gactccgacg agcggctttc agaaacggaa aaaattcagc
      841 cgaaagactc taatagcggc aaaacttcaa atctcaatcg ctccgctcct cctgccgcgc
      901 cgctgccgcc gcctaacgcc cagagaacaa ttcccgaacc gcaggcgaag ccgacgcccg
      961 cgcgaaatca gccgggcaaa atgccggttc ccgcgcctcc cgttttaata ccggcaaata
     1021 atcagcccgc caatcaaccg caaattcaga cgccaaatcc ggctctgccg cgaaaaagaa
     1081 gcttcagtat aattcgcgga tgcctgatta tgttttttct gcttattatt tctaccgtcc
     1141 tgcttttttt cggcgctcct tatgcgatta aggttatgcg cgaaacgcag caggaaagag
     1201 acagcgagtc gccgcgcttt ccgagccggt agacttgtat tgattctgaa tggattttgc
     1261 gtataaaact cgtacacaaa ttaaatcaaa attttaataa ctcgcaaaaa cgcgtcttta
     1321 ttttgtggca gagcttttgc ataaagcact gtttcaaatc aaggctgcct gctttacagc
     1381 ctatgacaaa tcaaaaggag aaatcattat gattaaacga ccgggcggcg aacttgcttc
     1441 aagaccgctg cattttatct ggattgccga cagttccggc tcgatggagc aggacggaaa
     1501 gattcaatcg ctgaacacgg cgattcgcga agcgattccg catatgaaaa aagtcgccga
     1561 agataatccg aacgcgcagg ttctggtgcg cgccgtcaaa ttttccaacg gcgcgcagtg
     1621 gcatatttcc caaccgacgc cggttgccga tttcgcgtgg acggatttag tcgccgaagg
     1681 cgaaaccgat atgggcaaag ccctggaact ggtctccgaa caattgaaaa tgccgccgat
     1741 gagcgaccgc gccctgccgc cggttctggt tcttgtgtcc gacgggcagc cgaccgacga
     1801 tttcgatgcc ggattaaaag ccctgatgga acagccctgg ggcaaaaaag ccgtccgcat
     1861 cgccatttca atcggaaagg acgcggacga cgaagttttg cagaaattta tcggcaactc
     1921 cgagctgaaa ccgctcgccg ccaacagccc tgaagcattg gtcaaacata tcaaatgggc
     1981 ttcgaccgcg gttctgaaat ccgcttcttc gcccgccgcc ggagccgaag ttccggctgg
     2041 gacggacacg gcaaacgtgc cgattccgac cgctgtgccg atcggagcaa ccgaagcgac
     2101 cggaacgacc ggaacgaccg gagcaaccgg gacgacaaat gcaactgccg ctactgcgcc
     2161 aaccggaacg cctgccggtt cgccggtcga cccgaatgac cagaatgcgg tcgacgacgt
     2221 ttggtaagga gcgcgtatga ccgaaaataa aaatgcgatt gagtggcgcg tcatcggcga
     2281 aaccgttccc ggcgcgtcgc atcttcgcgc cggagttcca aatcaggatt cgcttcttta
     2341 cgtccgcgaa tcgagccgcg cgctgccggt cgtcctttcc gtttccgacg gtcacggcag
     2401 cccgaaatgt tttcgcagcg accgcggctc gcagttcgcc gtgaaaaagg cggcgtatct
     2461 ggtgagcgaa tttttggacg agcggcgcgg caaatcggac gcgtcggaaa tcgaaagcca
     2521 agcggattat ttgtcaaaag agtttgtccg gcgctggcgc gaagcggtcg aagacgattt
     2581 gaaaaaagag ccgtttaccg aaaaggagtt tgaagcgctg gaaaaaaaat ccgatgcgaa
     2641 ggcgcgcaga ctcgtcgaag aaaacccgct tctggcttat ggcgcaacgt ctttaaccgt
     2701 agctgtagaa gaagatttta ttctttatat gcagctcggc gacggcgata ttctcaatgt
     2761 ttcggcgacg ggcgaagtta taaaaccgct tgcggaagac ccgcgattgc tcgccaacga
     2821 aacgacttcg ctgtgtctgc cgaaagcgga aaaggatttt cgctgtttag tccaaaagat
     2881 ttccgccgac gagtctccgg cgatgatttt gctttcgacc gacggttatc tgaattcctt
     2941 ttcaagcgaa accggatttt ttcaggcggg aacggatatt ttacagatgc tcggggcgga
     3001 cggcggcttc gatgccgtca acgataattt aaaagcctgg ctcgaagaag cgacccggat
     3061 gggcagcggc gacgattgca cggtcgccgt catttatcga cccgactttt tgaaaaaatc
     3121 tgaaaaaatc gaaaaatcga aggaaattga aacgacgaca atcgaggcgc cgcccacgcc
     3181 ggaaacttca caggcgattg ccgcgctgac cgattctgaa acggttgcgc ccgaaaatat
     3241 ggaagacgtg accgtcacga ttacgattaa aaaagataaa aataaaaaag ccgcatcgcc
     3301 cgctcaaccg gattcggagc aaaaagcaca tccggacgag cataaaaaca gcgtcaaaaa
     3361 agtttcatga acgagctgcc gcgccgaaaa cttatagaaa tcgtcgcccg caaagggcgc
     3421 tcaataatcg agaatccggg acgcgtcaag ggacttctgc gagattactg cggcgcgtac
     3481 cggcgggaaa tctcggtact ggcgatggct ctggaggaac acgccgtgcc ggatttattg
     3541 tccgcgccgg cgagcctgcc gcgcaaagtt ttactggctc ggctcgcgcg gcgattatgc
     3601 gacaatctgg ctttgtccga agaagcggca atctggtcga ttgaaagctg ggcgctggcg
     3661 ttaagcgtga tttcagccgc cgaactcgaa acaaatgaaa ccgcgccggc ggcggcattg
     3721 cacgtcgtta aaccgcgcga agaaatttct tcgaccgccg ccgcggcgtc acaaaaccga
     3781 ccggcaaaca gtgttcaatc aaaaccgcgg gcgaattcat ttattgtcgc cgcagacggc
     3841 agcggcgatt tcaaaactat cggcgaggct ttgcaaaagg tcgcgccgaa cgcgcgcctg
     3901 ctggttcgcg aaggcttcta cgatgaaagc attattctcg ataaaaacat cgaaattctg
     3961 ggtgacggcg cgttcgataa aataattgtt cgaagcgtga attcgagctg tattctgatg
     4021 cagaccgata aagcgtcggt gcgcggtttg agcttgcgcg gaagcggcgc gcgcggcggc
     4081 aaagcctttt tcgccgtcga tattccgaaa ggtgaattga ttctggaaag ctgcgacatt
     4141 tcttcggatt cgctttcgtg cattgccgtt cacggcgtga attcaaaacc cgcgataaaa
     4201 aactgccgga ttcacgacgg cgccgattcg ggtttatatt tttatgataa cgctcgcggg
     4261 cgcgtggaaa attgcgacgt ttatcgaaac gccaatgtcg gcgtcgcgat aactcagggc
     4321 gcaaatcctg cgctcgcaaa ttgccgcgtc tttcagggaa ataacggcgg cgtcgtggct
     4381 tggggaaacg gcgcggcggg cgtgattgaa aattgtcata ttttcgggca tcggctggcg
     4441 aatatcggaa tcagcgaata cgccaatccg atttttcgac gctgtgaaat ttacaacggt
     4501 caggacacgg gcgtttacgt tcacgccaat ggattcggca cgctcgaaga atgtaatatt
     4561 tataaaaact cgaaagccga agtcggcgtt tctcagggcg gaaattccat tttccggcgc
     4621 tgcgcgattc acgacggcga aaattcgggc gtcattctgc aaaatcgagg tcggacgacg
     4681 ctcgaaagct gcaatattta tgacaacgcg gacgccggcg tggcgattta cggggaaagc
     4741 acggtcatta tccggcgctg caatatccat cgcaacggca aagttgccgt cagagttaaa
     4801 gaacaaagcg cggcgcgagt ggaaaattgt gatttgaggg gaaatcgtct tgccgcctgg
     4861 gaaaccgaat acggcattac gctggaagaa agcggtaacc gcgactaaca ggcaaatgta
     4921 attggagaaa ttaaaaatgg atcagatttt aaaaccgggt caggtgattc aatgccagcc
     4981 atcgggaatg ccctgcacga ttgaaacgct gctcggcgcg ggcggacagg gcgaagttta
     5041 taaagccgaa ctcggcggcg aagcggtcgc cgtcaaatgg ttttttccgc atatggcgac
     5101 gcccgaacaa ctggcttcga tgcaattgct cgtgcgcaaa ggaccgccga gcgagcattt
     5161 tctttatccg atggagatta cgacggcggc gggcgttcag ggtttcggct acataatgcc
     5221 gctgcgaccg aagcgtttta aaaacatcgt cgatttgatg acgcggcgca tcgagccgac
     5281 ttttcgcgcg ctcgccaccg ccggtctgaa tctctcgcac aactatctgc tgcttcactc
     5341 gcagggctta tgctaccgcg acatttcttt cggcaacgca tttttcgacc cggacaacgg
     5401 cgatatttta atcgcggaca acgataatgt ttcggtcgac ggcgcgggag ttttaggcgt
     5461 tttgggaacg ccgcgcttta tggctccgga aatcgtccgc ggcgaagctc ttccgagcac
     5521 gcaaaccgat ttgttttcgc tttcggtttt gattttttat atgctgatgg tcgcgcatcc
     5581 cctggagggc aaaaaagaag ccgccatcaa agctctggat ttgccggcga tgacgaaact
     5641 ctacggcaag gaaccgattt tcatcttcga cccgaacgac gattccaacg cgcccgttcc
     5701 gggcattcac gacaacgcgc tcgctttctg gcgaatttat ccgcaatttt tacgcgacat
     5761 ttttatccgg gctttcacaa tcggcattcg cgacccgaaa aacgggcgcg ttcgtgaaag
     5821 cgagtggcgc gggacgatgg tcgaactgcg cgatgtgatt ttctattgcc cgcattgttc
     5881 gctggaaaat ttttatgatt cgcaggcttt aaaagccagc ggcggaaatc cgggtctctg
     5941 ctgggcttgc gccgcccaat tacggcttcc gccgcggata cgcatcggga aatcggtcgt
     6001 catgctcaat cacgatacaa aacttttccc gcatcacacg gacgaccaga aactgtacga
     6061 tttttccgcg ccggttgccg cggttgccgt tcatccgaca aacccgaaca tctggggact
     6121 gaaaaatctt tcgggcgaca aatggtttat taccgccgcc gacggaagcg tcaaagatgt
     6181 cgagccgcag cgaagcgtta ctctagcggt cgggactaaa attaattttg gaaaatcgga
     6241 aggcgaaatc agattgtaaa aagaaatccg tttccggatt atttaaactt caaaagaagt
     6301 tgctgtcgaa tccctcgacg acgccatctc ttttttcacg gagacaggac tgaaacttga
     6361 agggacgcgc cacggtcgaa ggtgaatggg cgggtcgcgt gacaggactc ggttctcaac
     6421 gttttgagtg aaagatattg agtaaacata tttaatacat aaccccagac atcgaagagt
     6481 aaagttttgt ttctcatacg cgtatttcaa aagtctttaa taaaaatttc aaactaactt
     6541 tcacgtaact ttcaagactt tcagggcgaa agtttcttac ctttatctac ggataaggga
     6601 gataatcaca atttgcggtt tgctggcgtt tgctcagggc agccgcagca gcgctttggg
     6661 ttttcttata atactaaatt ttatgagccg atcagaggaa cgataaccgg atgaggattt
     6721 tattttatct gctgccttta cttgtcatag gttggacggt gtggggaata tttgcttata
     6781 aagagctaaa aaccgctctt aaaaagggcg agcgcggagc actcctcaga gagtataaaa
     6841 taacgattct gggagagatt atcggaggac ttacagcgtt tggagctatc ggatatgaag
     6901 ttttcaaatc ctcggatacg tttggtatca gttttgggga aggaggaaat tttctaattg
     6961 cggcggcggc tgtcggaagt cttttaggct taattatcgt accgttgctt gctaagttcg
     7021 gtaagaatgt aatggtcggc gacatcgagg ctttgctgcc gcgcacaaaa aacgaaaagc
     7081 tgtggtttat tcttgtagct ctcagcgcgg gcgtatgcga ggagctgata ttccggggat
     7141 ttggtcttag attattcgag tcttttggat ttaccgggat tgctttgctt attgtaactg
     7201 ccgtcgtatt tgggttagtg catatttatc agggatttct cggagtaata acatcagcaa
     7261 taatgggcgc tttatttaca ctcatatata tccagaccgg ttctctgtgg tgggcaatcg
     7321 cggcacatat ttttatagac ttacgtgtcc tgctgctagt cgctttcgct aaaaaacatt
     7381 gagtttaacg tggattttga cgctcaatca acagaagcag gtatattacc ggataaattt
     7441 ggatttgcat ttaatgccgt tgcagattta gaaggtgctt gatttaagtc cttatattct
     7501 ctcacccata tttatgtgac agttacaaaa acgcctcttt tcggagtctg aaaatacaga
     7561 agagttacga gagagcttaa agcgcggctt ccggcggtaa aattcgccga agaaatgtaa
     7621 taatgttatt ggaaattaca accttaattg ttgcgagccg gcttgcagaa gtggaaaaga
     7681 tgcttttgat gggagcaccc cgtaattcga gaaactcgaa ccggacataa agaatgaagt
     7741 tcataacgat aaaaataagt aaagtatgag cagaggattt gttaaagaag gcgatcagga
     7801 agaaattcct attttaacgc ccagagctta cttgcctgat ggcgtgacca actacgttac
     7861 tcaagttggt atgaatgaac ttttggacga aaaacagatg ctcatgggcg agatagagaa
     7921 tctggacatg acaaatgaaa atgaaagacg aatcgcatcg aatcatttaa acgcaaaact
     7981 gaagttgttg aatgaaagaa ttcatactgc ggaaataata gacctggaga aacaatcacc
     8041 ggacaaagta tggtttggcg caaccgtaac gctaaagacg gataacgaaa caaagcttca
     8101 aaaatatcaa attgtcggtg tcgatgaagc cgatgtttct caaggaaaaa tttcatttat
     8161 ttctccaatt gccaaaatat tgatgggtaa ggaagccggt gaaaaagcaa tattaaaact
     8221 tgcaaatgga aacaggacat atgaaattat agaaatcgtc tattaaagtc cggttctgaa
     8281 ctcctgtcga aaacgccgag tttcgagaca acaaaatagt gataaaatcc gtctcggaaa
     8341 cgccccttgt cgttttcatc aaactctgcg aataaaggcg gctgagactt gcttttttat
     8401 ttcaaacttt gcgaataaat ata
//
LOCUS       DASWNE010000226         2070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_466192, whole genome shotgun sequence.
ACCESSION   DASWNE010000226 DASWNE010000000
VERSION     DASWNE010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466192"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..1097
                     /locus_tag="VGB68_04610"
     CDS             84..1097
                     /locus_tag="VGB68_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048542.1"
                     /translation="MSNIFTITTAQQTLKANENGQATAVFTVTNAASRPVRYFVKIKP
                     LGNTEAQWLMIEGETERDFPAGGTHQFTVNFNKPKSSTAPAPPSAPQPAEAFPFRLDA
                     ISAINPDEDFTEGPVVTVEIPEQKVEPKKSFPWWILIVVGVLLVVGIIVAVLMYRGGG
                     GDGAPTPTPTPTPAVTRVIYDFIENAPAAVWKNDLDRILPINGPSESTGFAQIIRNAP
                     MESDFAEAVALHTHPRWEVNGAVTGTYNLPEPINAKDRFRARIGFLKNGRAGNLKVRL
                     LLNGTVIAELPKAYDGALREFEVDLGRYQGQTGKIALQALAVPTSAQGWICWINPRIE
                     RVP"
     gene            1154..1969
                     /locus_tag="VGB68_04615"
     CDS             1154..1969
                     /locus_tag="VGB68_04615"
                     /inference="COORDINATES: protein motif:HMM:NF025653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAN domain-containing protein"
                     /protein_id="HEY0048543.1"
                     /translation="MANIFTITTPTQTLKADASGAATTVFTVTNTTSRPVRGIAKVKP
                     LGNTEAQWLMIDGEAERDFPAGGTHQFTVNFNKPKPATPPAPPSAPQPAEFFPFRLDA
                     ISTTNPDEDFAEGQVVTVEIAERKAEPKKSFPWWILIIVGVLVIGTIAAVFLLRDKGT
                     DDNENTATPTATGTPAATPTRTTSIPTPIMPTPSKTRPKIVRMAPKEYGKDRPGSDFQ
                     YFVAPSLQDCENACRENAACKAFTFGNGSNCWLKNAKPAPVNNPNFISGIKIE"
BASE COUNT          538 a          540 c          556 g          436 t
ORIGIN      
        1 tttgacgaaa aaggtgtttc gcatctttca ttagctgaat tcagtttatg attactagca
       61 ccaatggtta attaaagaat attatgtcga atattttcac aatcacgact gcccagcaaa
      121 cactgaaggc aaatgagaac ggacaggcga cagccgtttt cactgtcact aacgcggctt
      181 cgcgcccggt tcgctacttc gttaaaatca aaccgctcgg caacaccgag gctcaatggt
      241 tgatgattga aggcgaaacc gaacgcgatt ttcctgccgg cggcacgcat cagtttaccg
      301 ttaatttcaa taaaccaaaa tcgtcgacag cgcccgcgcc gccaagcgcg ccgcaaccgg
      361 cagaagcttt cccgtttcgt ctggacgcga tttccgccat taacccggac gaagatttca
      421 cggaaggacc ggtcgtgacg gtcgagattc cggagcagaa ggtcgagccg aaaaaatctt
      481 tcccgtggtg gatactaatt gtcgtcggag ttttgctggt ggtcggaata attgtcgcgg
      541 ttcttatgta cagaggcggt ggaggcgatg gcgctccgac gccgacgccg acgccgactc
      601 cggcagtcac gcgcgtaatt tatgatttta tagagaatgc gccggccgcc gtctggaaaa
      661 atgatttaga tagaattctg ccgataaacg gtccgtcgga atcgaccgga ttcgctcaga
      721 ttatcagaaa cgcgccgatg gagagcgact tcgcggaagc tgtcgcgctt cacacgcatc
      781 cgcgctggga ggtcaacgga gccgtcaccg gaacctacaa tttgccggag ccgattaacg
      841 ccaaagaccg ttttcgcgcc agaatcggtt ttttgaaaaa cggcagagcc ggaaacctga
      901 aagtcagact gcttttaaac ggaaccgtca tcgccgaatt gcccaaagct tacgacggcg
      961 ctttacgcga atttgaggtt gacctcgggc gctaccaggg acaaacagga aagatagctt
     1021 tgcaggcatt agccgtgccg accagcgcgc agggctggat ttgctggata aatccgcgca
     1081 tcgagcgcgt gccgtagcgg attttataag taaaagaatt ggcagcggcg aaataaattt
     1141 cgggaagaaa actatggcaa atatttttac aatcacgacg ccgacgcaaa ctttgaaggc
     1201 ggacgcaagc ggcgcggcaa cgactgtttt caccgtcaca aacacgactt cgcgtccggt
     1261 tcgcggcatc gccaaagtca agccgctcgg caataccgag gctcaatggc tgatgatcga
     1321 cggcgaagcc gagcgtgatt ttccggctgg cggcacgcat caatttaccg tcaacttcaa
     1381 taagcccaaa ccggcgacac cgcccgcgcc gccgagcgcg ccgcagccgg cggaattttt
     1441 cccgtttcgg ctcgacgcga tttcaacgac taacccggac gaagattttg cggaaggtca
     1501 ggtcgtgacg gtcgaaatag ccgaacggaa agccgagccg aaaaaatctt ttccgtggtg
     1561 gattttgatt attgtcggag ttctggtaat cggcacaatc gccgccgttt ttttgcttag
     1621 ggataaagga accgacgaca atgaaaatac ggcgacgccg acggcaaccg ggacgccagc
     1681 cgcaacgccg acccggacga catcgatacc gacaccgata atgccgacgc cgtcgaagac
     1741 gcgaccgaaa atcgtcagaa tggctccgaa agaatacggc aaagaccgtc cgggctcaga
     1801 ctttcaatat tttgtcgcgc ccagtcttca ggattgcgag aacgcctgcc gggaaaacgc
     1861 ggcgtgtaaa gctttcactt tcggaaacgg ctctaattgc tggcttaaaa acgcaaagcc
     1921 cgcgccggta aacaacccga attttatttc cgggataaaa atcgaatagc tatgcgggcg
     1981 cggcgagtca tcgactgcgg gcgaaaaacg ccgtagtttt ccggaattga ttggtagata
     2041 tgaataaaat cgtcattatc gttttggttt
//
LOCUS       DASWNE010000227         3120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_537527, whole genome shotgun sequence.
ACCESSION   DASWNE010000227 DASWNE010000000
VERSION     DASWNE010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537527"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..1240
                     /locus_tag="VGB68_04620"
     CDS             185..1240
                     /locus_tag="VGB68_04620"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY0048544.1"
                     /translation="MSNETEEESPPPITPEEFLAERKRSMRRRSWWAIGIGAVLVALH
                     LFLFALAIIFKEQLQAQYPEEFGGEISWKILFQRLSFLLGLFALTAGIWGLYEAKRIT
                     LEDLIPSPEAIEFLQQARDTVPYFSYILVGCLVVVTLFQLKIEDVSSYINFGEQSTKI
                     AGLVKPLVWEGEWWRLLTYAALHGIFPLHLYFNAQALYGFGSLIEFLSNRAHLGIVFL
                     LSIIGGGLLSLFFMPEVQSIGASGGIMGLIGYMAIYGYRRKRQLPPEFLRSMLVNIGF
                     IAAFGIIAYQIVDNFGHFGGLLTGAIYGFLQIPRDLHKNPRQAGALTEAFGLISMGVF
                     VFVSILTVLLLLQYIKF"
     gene            1314..1937
                     /gene="trmB"
                     /locus_tag="VGB68_04625"
     CDS             1314..1937
                     /gene="trmB"
                     /locus_tag="VGB68_04625"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEY0048545.1"
                     /translation="MPRVRVHQHVNPLAPYFRFISKPLDLTKIFVNPDLPLHLDIGAA
                     RGRFLLKMAEVKPEWNFLGLEIREPLVEEANRLAAEKNLQNLHYEFTNATISLGDLLK
                     NFPEDVLRAVTIQFPDPWHKKKHAKRRMVNREMADAVARHLAAAGTIFIQTDVEFLAE
                     EMFEIFRGFEELREIETTENPFPLKTEREKAVEEKNLPVYRAMFEKI"
     gene            complement(2091..>3120)
                     /locus_tag="VGB68_04630"
     CDS             complement(2091..>3120)
                     /locus_tag="VGB68_04630"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY0048546.1"
                     /translation="EGEMAGVMAHEISHVALRHATAQATKQSNPLNQILGGAAILGGA
                     ILGGQAGAVLGQSIYKGVFVNPYSREYETQADVLGSQIMARAGYDPRDLANMFQTIAQ
                     QGGSRSPEWLSSHPDPGNRFNNINREAALLRVSPEPIKITQGFLSTKRRFQGMPRAQT
                     MEEIARNPQNNRGQGQAVNGRYETRVQTPSSRYRTYSSGSQISLSVPENWGTVSETET
                     AVWFAPEGAYGNNGITHGAMIGIAQSSGNLSQATQNYVQGLLQNNDYLQQQNNYSRAT
                     LAGRSGLATVLSGRSPITGRTEIVTVYTTSLQNGYLFYAITVVPQNEAATYNYAFRNM
                     LSSLRLNA"
BASE COUNT          709 a          770 c          799 g          842 t
ORIGIN      
        1 atagtcgaaa tcaaatcaat tattgatatt ctcaataaat aaaaaaggct gtccaaaacg
       61 gacagtcttt taaacgttct ctcgcggact ttacaggaaa agccgtttta agttttagat
      121 tttcgactgc cgcgcggaaa attcttgcaa tctggagttt agcctctaaa atctaaaatt
      181 gaatatgtcc aacgaaacgg aagaagaatc gccgccgccc atcacgcccg aagaatttct
      241 ggcggagcgc aaacgctcga tgcgcaggcg gtcgtggtgg gcaatcggca tcggggcggt
      301 gctggtcgcg ctgcatctgt ttctgtttgc cctcgccatt attttcaaag agcagcttca
      361 ggcgcaatac ccggaggagt tcggcggcga aatcagctgg aaaattcttt tccagcgctt
      421 atccttcctg ctcggacttt tcgcgctgac tgccggcatc tggggacttt acgaagcgaa
      481 aagaatcacg ctcgaagatt tgattccgtc gcccgaagcc atcgaattcc tgcagcaggc
      541 gcgcgacacg gttccttatt tttcatatat tctggtcggc tgcctggtcg tcgtgacgct
      601 ctttcagttg aagatagaag atgtcagctc ttatataaac ttcggcgagc aatcgacgaa
      661 aattgccggt ttggtcaagc ctttggtctg ggaaggcgaa tggtggcgtt tgctgactta
      721 cgccgctctg cacggaattt ttccgcttca tctttatttt aacgcgcagg cgctttacgg
      781 tttcggcagc ttgattgaat ttctctcaaa ccgcgcgcat ctgggaatag tctttctttt
      841 gtcaatcatc ggcggcggtc ttttaagctt gttttttatg cctgaagtcc aatcaatcgg
      901 cgcttcgggc ggcattatgg gtttaatcgg atatatggcg atttacggtt atcggcgcaa
      961 acgccaattg ccgccggaat ttttgcgaag tatgctggtc aatatcggtt ttatcgcggc
     1021 tttcggcatt atcgcttacc agatagtcga taatttcgga catttcggcg ggcttttaac
     1081 gggcgcgatt tacggctttt tgcagattcc gcgcgattta cataaaaatc cgcgccaagc
     1141 cggcgcgttg accgaagcat tcggtttaat ttcgatggga gtttttgtct tcgtctcaat
     1201 tttaaccgtt ctgctgcttt tgcaatatat aaaattttaa aaattcaaga ttcaggattc
     1261 aagattgcca ttttgaatct tgaatcctga atctaaaatc taaaattcag attatgccga
     1321 gagttcgcgt tcaccagcac gtcaatccgc ttgcgcctta tttccggttc atttccaagc
     1381 cgctggattt aacaaagatt tttgttaatc cggatttgcc gctgcatctc gacatcggcg
     1441 cggcgcgcgg gcgttttctg ctcaaaatgg cggaggttaa acccgaatgg aactttctcg
     1501 gtctggaaat ccgcgagcct ctggtcgaag aagcaaatcg tctggcggcg gaaaagaatt
     1561 tgcaaaatct gcattatgaa ttcaccaacg cgacaatctc gctcggagat ttattgaaaa
     1621 actttcccga agacgttttg cgggcggtga cgattcagtt tcccgacccg tggcataaaa
     1681 agaagcacgc gaaacgccgg atggtcaacc gcgaaatggc ggacgccgtc gccaggcatt
     1741 tagccgcggc gggaacgatt tttattcaga ccgatgtcga gtttctggcg gaagagatgt
     1801 ttgaaatttt tcgcggattt gaagaattaa gggaaatcga aacgaccgaa aatccttttc
     1861 cgctgaagac cgagcgcgag aaagcggtcg aggaaaaaaa tctgccggtt taccgggcga
     1921 tgttcgaaaa gatttagcca cgcaggaaca tgaaaaagaa ttaacaggga tgacaggata
     1981 aacagggatt gaaaacatta tctttaatcc ctgtttatcc tgtcatccct gtatttttct
     2041 ttcttatttc gttttctttt cgtgttcttt cgcggctaaa aatctttcgc ttaagcgttc
     2101 aggcgcagcg agctgagcat attgcgaaaa gcgtaattgt aagtcgcggc ttcattctgc
     2161 ggcacgaccg taatcgcgta gaacaaataa ccgttttgca aagaagttgt gtaaacggtg
     2221 acgatttcgg ttctcccggt tatcggcgag cgtcccgaca gaacggtcgc caaaccgctt
     2281 ctgccggcaa gcgtcgcgcg cgaataatta ttttgctgct gcagataatc gttgttttgc
     2341 agaagtccct ggacgtagtt ctgcgttgcc tgcgacaaat ttccgctgga ttgggcaatg
     2401 ccaatcatcg cgccgtgcgt gatgccgttg ttgccgtaag cgccttcggg cgcaaaccag
     2461 actgccgttt cggtttcgga aaccgtgccc cagttttcag gcacgctcaa actgatttgg
     2521 cttccgctcg aataagttcg atagcgtgac gaaggcgttt gcacgcgcgt ttcgtaacga
     2581 ccgttgacgg cttgtccctg accgcgatta ttctgcgggt tgcgggcgat ttcttccatc
     2641 gtctgcgcgc gcggcattcc ctgaaaacgc cgtttcgtgc tcaaaaatcc ctgcgttatc
     2701 ttaatcggtt cgggcgaaac gcgcagcaga gcggcttcgc gattaatatt attaaagcga
     2761 ttgcccgggt ccgggtggct gctcagccat tcggggctgc ggctgccgcc ctgctgcgca
     2821 atcgtctgaa acatattcgc caaatcgcgc gggtcgtaac cggcgcgcgc cataatttgc
     2881 gagccgagaa cgtcggcttg cgtttcgtat tcgcggctgt aagggttgac aaaaacccct
     2941 ttataaatac tctgcccgag caccgcgccg gcttgaccgc cgagaatcgc gccgcctaaa
     3001 atcgccgcgc cgccgagaat ctggttcagc ggattgctct gtttggtcgc ctgcgccgtc
     3061 gcgtggcgaa gcgcgacgtg gctgatttcg tgagccatca cgcccgccat ctcgccttcg
//
LOCUS       DASWNE010000228         8401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_7141, whole genome shotgun sequence.
ACCESSION   DASWNE010000228 DASWNE010000000
VERSION     DASWNE010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7141"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1356
                     /locus_tag="VGB68_04635"
     CDS             <1..1356
                     /locus_tag="VGB68_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY0048547.1"
                     /translation="KSLGITAIWTTPVYDNNDRPDTKEVYEGVPETTGYHGYGAIDMY
                     AVDEHLGDTAKLRELVDKAHALGIKVIQDQVANHTGPYHVWANDPPTPTWWNGTLENH
                     LSNNWQKWTAMNPRATYQTQKRNIDGWFIDILPDFNQDDPEVEKYLIQNSIWWLEMNG
                     FDAIRMDTLPHVPRSFWEKWGGAIKKEYPGVNILGELFDSDPALISYFQTGRRGHDAV
                     DTQIDTLYDFGLFYPIRNAFAKGKPIREVSQMFSKDWLYPNAEVLTTFLGVHDMPRFM
                     HEEGATTEGLKLAQTLIMTSRGTPLLYYGDELAMRGGGDPDNRRDFPGGFPGDARNAF
                     TNAGRTAEEGDVWNHLALLGKLRGELEPLRKGKSVDLLDEEQQMAYARVTDRQAVLMI
                     FNNDAKAMEVSFEVSMIKQFPANSTLSDRLGKVSDVKISNGNVKVTIPARTAGIFTVR
                     Q"
     gene            1375..2094
                     /locus_tag="VGB68_04640"
     CDS             1375..2094
                     /locus_tag="VGB68_04640"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0048548.1"
                     /translation="MNTKDLSEQTALITGGATGIGRAFAEALLENGISAVIIASRRRS
                     VLDEAAAELNSRFGAGKVIAYAFDVRHRAETENLVRFALEKCGAVDVLINNSGLAVPE
                     TVTEITGAGWDAVMETNLRGAMWLTQMLLPKMLEQDFGDIINVSSQAGKNGYADVPLY
                     CASKFGLLGFAESLRDDVRKRGANVRVFNFCPGLVDVETPKKQRARSGFIHVGNMAKT
                     LIYALSLDRNVILEDINIYSR"
     gene            2230..3555
                     /locus_tag="VGB68_04645"
     CDS             2230..3555
                     /locus_tag="VGB68_04645"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0048549.1"
                     /translation="MSFGFLGIQIGFGLQQANMSPIYKYLGAEESSLPYLWLAGPVTG
                     LLIQPVIGAMSDRTWNRLGRRRPYFLIGAVMSSICLLLMPFSSAVWMAAGLLWILDAS
                     INTSMEPFRAFVGDKLPEEQRTTGFVTQSCFIGVGQTVANALPVSLASLGIAGALASG
                     IPYATYYAFIIGAVVFLAAVLWTVFSTREYPPEDMEEFRRKQAEGNIVGGIVTEIWDA
                     LKNMPTTMKQLAVVQFFTWFALPCMWQFYGLAVARHVYLAPDEKSPLFAAGTEWGGLS
                     FAVYNVVCFLVAFLLPPLAGATSRKTVHFICLTLGGLGLLSTYFAHDKYFLWLGMAGV
                     GVAWASILSMPYVLLAGAIKPSRMGVYMGVFNLFIVIPQIVMSLVVPQLYKNVLGADP
                     LNVVMLGGLVMLLAAVSVLLVRDVGAANIETSVPTASGIAGAEREDIKG"
     gene            3611..4978
                     /locus_tag="VGB68_04650"
     CDS             3611..4978
                     /locus_tag="VGB68_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY0048550.1"
                     /translation="MLILKKVLGTLALTIIFSNFIFTQQAQRDFSKENARPSPDWVKD
                     AVIYEIFPRAFSQKGDFNSITADLDRLKNTGVTVLWLMPVHPIGREKAKGTIGSPYAV
                     KDYYAVNPDYGTSADLKRLVSEAHRRGLKVIIDIVANHTAWDSVMMKNPAFYTKNERG
                     EIIPPVPDWADVADLNYDNPQLRKYIIEMLKMWVRDYDLDGFRCDVAGFVPTDFWETA
                     RAEVDKIKPDTIWLAEWESPDLLVKAFDLDYSWANHSALTEVLQGNKPASEIRRVWEE
                     QKAKFPKNALRMRFSDNHDERRAIARFGEKGALAAQALVFTLDGVPMFYNGMEAGDTT
                     ESGAPALFEKLPVFWRIQERRPDFPKFYKAMTELRRNSVALRRGDLVWLKNSDETRVL
                     TFLRRQGDEEILVAINMTNAPFFGSVEAGGNFEDVTPNLEKKNVSLPAVSLDAFGYRI
                     FRKKI"
     gene            5107..>8401
                     /locus_tag="VGB68_04655"
     CDS             5107..>8401
                     /locus_tag="VGB68_04655"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEY0048551.1"
                     /translation="MFALIFFSFTAAAQTLAPGAPGKDAQWASAGKQGVGTSATLESK
                     VWFTLQGGALTEVYYPDVTVANVHLLQFVVVNPKTRKVETERDDANHQIKVLRPDSLT
                     FRQINTGKSGEWKITKTYTTDVERDSVLIDVKFESKNKNLNLYVYYDPSLGNSGMQDF
                     ASPALGTGGGADSKLVAQNEKPPVIASALSFSGEISEQTSGFYQISDGLEQLRQYGKI
                     VKRYDKAGTGNVVQMARILKPFHFTTVLSFDPNSSVKASENSIYSLNKGFQRCLAEYE
                     KGWSDYVKTLRRVEPKYQAQFNMAAMILKAHEDKANRGANIASLTVPWGGGENANENN
                     VGGYHLVWSRDLYQVATAFMALGDKAAAERALDFLFKVQQKPDGSFPQNSWLDGKPFW
                     GSLQLDEVAFPLILAYQLGRFDKATYTNHVKKSADFLVKTGPRTPQERWEEEAGFSPS
                     TIAAEIAGLVCAAEIASRNGDEASATVYLATADDWARNVERWTATTTGLYGDGVYYLR
                     ITQNGAPDKGEKIELNNGAGFFDERAIVDAGFLELVRLGIKSADDPLIRKSVKVIDEV
                     IRVKTPNGDAFYRYNHDGYGEMDDGRRWNWDGKYTGKGRLWALLSGERGQYELALCGL
                     QKFIVDGTGCSDLVKYERLEAMQKFANEGLMIPEQIWDKKEAPKADSQFSPNLKFGEG
                     TGSATPLAWSMAQFIRLAVNLREGRNPDTPDVVYNRYARSGIPAQTSNFGGTDEEIVL
                     PVNAGQKFSFKRQAMAGAKVAMSFRGETRLLPINEKGEISFEIIAPDAEEIAIVGIIT
                     PDGASAFERVKLRSADAKITGQGNYVFSPPRENEPSPGIFGLKGAEKTDVIFVYRGAG
                     KSVEIAGDFTGWRPHGRKFQNLGSCQVLILPFDNGAKFEYKLVVDGKWMTDPLNPHKI
                     DNGVGGENSVFTMPGYKPTVWDKEQSFKMEGVNEKFIIEGRHEAFDLQTKNFGSRRIK
                     VYLPIRYNASKDRYPVLYFQDGSDYLQRAKAAVQQENLVGAGKIKPFIMVFIDPRERT
                     KEYWANDAWADFVANEVVPEIDKRYRTIQNRDGRALLGASLGGITSVWIGLRHPDKFA
                     RIGGQSSSFW"
BASE COUNT         2037 a         2072 c         2412 g         1880 t
ORIGIN      
        1 aaaagtttgg gaatcacggc gatctggacg acgcccgttt acgacaacaa cgaccgaccc
       61 gacacgaagg aagtttacga aggcgtgccc gagacgaccg gctatcacgg ctacggcgcg
      121 atcgatatgt acgcggtgga cgagcatctg ggcgatacgg cgaagctgcg cgagctggtc
      181 gataaagcgc acgctctggg aatcaaagtc attcaggatc aggtggcgaa tcacaccgga
      241 ccgtatcacg tctgggcaaa cgacccgccg acgccgacct ggtggaacgg cacgctcgaa
      301 aatcacctgt cgaacaactg gcagaaatgg acggcgatga acccgcgcgc cacgtatcaa
      361 acccaaaagc gaaacatcga cggctggttt atagatattc tgccggactt taatcaagac
      421 gacccggaag tcgaaaaata cctgattcaa aattcgattt ggtggctgga aatgaacggc
      481 tttgacgcca ttcggatgga cactctgccg cacgttccgc gctcgttctg ggaaaaatgg
      541 ggcggcgcga taaaaaaaga atatccgggc gtgaacattc tgggcgagct tttcgacagc
      601 gacccggcgc tgatttctta ttttcaaacc gggcggcgcg ggcacgatgc cgtcgacacg
      661 cagattgaca cgctttacga tttcgggctg ttttacccga ttcgcaacgc tttcgcgaag
      721 ggaaaaccga ttcgcgaagt ctcgcagatg ttttccaaag actggcttta tccgaatgcg
      781 gaagttttga caacgttctt aggcgttcac gatatgccgc gttttatgca cgaggaaggc
      841 gcgaccactg aaggcttgaa gctggcgcag accttgataa tgacctcgcg cggaacgccc
      901 ttgctttatt acggcgacga gctggcgatg cgcggcggcg gcgacccgga caaccggcgg
      961 gattttccgg gcggctttcc cggcgacgcc cgaaacgctt ttacgaacgc gggcagaacg
     1021 gcggaagaag gagacgtctg gaatcatctc gctcttttgg gaaaattgcg cggcgagctt
     1081 gagcctttgc gaaaaggaaa atccgtagat ttgctcgacg aagaacaaca gatggcttac
     1141 gcgcgcgtga ccgaccggca agccgttttg atgattttca acaacgacgc gaaagcgatg
     1201 gaagtttctt tcgaagtttc gatgataaag cagtttccgg ctaattcgac gttgagcgac
     1261 cggctcggaa aagtttcgga tgtaaaaatc agcaacggca atgtgaaagt cacaattccg
     1321 gcgcgaacgg cgggaatttt tacggtcagg caataaattt tttactataa gctaatgaat
     1381 acgaaagact tatccgagca aaccgcgctc atcaccggcg gcgcgaccgg aatcgggcgc
     1441 gcgtttgccg aagctctgct ggaaaacggg atcagcgcgg tcattatcgc ttcgcggagg
     1501 cggagcgttc tggacgaagc ggcagccgaa ttgaattcga gattcggcgc gggcaaagtc
     1561 atcgcttacg ctttcgacgt tcgccatcgc gcggagacgg aaaatctcgt ccgcttcgcg
     1621 ctcgaaaaat gcggcgcggt cgatgttttg ataaataatt ccggtttagc cgtgccggaa
     1681 accgtgaccg aaattaccgg cgcgggctgg gacgcggtta tggaaacaaa cctgcgcggc
     1741 gcgatgtggc tgacgcagat gcttttgccg aaaatgctgg agcaggattt cggcgacatc
     1801 ataaacgttt cgtcgcaggc gggaaaaaac ggctacgccg acgtgccgtt gtattgcgcg
     1861 tcgaaattcg gtctgctcgg tttcgccgaa tccttgcgcg acgacgtgcg caagcgcggc
     1921 gcaaacgtcc gcgtttttaa tttctgtccg ggtctggtcg atgtcgaaac gccgaaaaag
     1981 cagcgggcgc gcagcggatt tattcacgtc ggcaatatgg caaagacctt gatatacgcg
     2041 ctttcattag accgcaacgt aattttagaa gatataaata tttattctcg ttgaaacgaa
     2101 aaacaggagg ctgaaggaca gaagccaaaa gtcgtgagcc gaaaaaacta ccggctgccg
     2161 actgccggct actggctacc agaacttatg caaaacaaac cgcgacttag tttttggcaa
     2221 atctggaata tgagtttcgg atttctcggc attcaaatcg gcttcggctt gcagcaggcg
     2281 aatatgtcgc cgatttacaa atatctcggc gcggaagaaa gcagcctgcc gtatttgtgg
     2341 ctcgccggac cggtgacggg tttgctgatt cagccggtta tcggcgcgat gagcgaccgg
     2401 acgtggaatc gtctcggcag acgccgcccg tattttctta tcggcgcggt tatgtcgagc
     2461 atttgtcttc ttctgatgcc gttttcgtcg gcggtttgga tggcggcggg acttttgtgg
     2521 attctggacg ccagcattaa caccagtatg gagccgtttc gcgcttttgt cggcgacaag
     2581 ctgcccgaag aacagcgcac cacgggtttt gtcacgcagt cgtgttttat cggcgtcggt
     2641 cagaccgtcg ccaacgcttt gccggttagt ctggcgagtc tgggcattgc cggtgcgctg
     2701 gcgagcggta ttccctatgc gacgtattac gcgtttatca tcggcgcggt cgtttttctc
     2761 gccgcggttt tgtggacggt tttcagcacc agggaatacc cgcccgaaga tatggaagaa
     2821 ttccggcgca aacaagccga aggcaacatt gtcggcggca tcgtcacgga aatctgggat
     2881 gccttgaaaa atatgccgac gacgatgaag cagttggcgg tcgtgcagtt ttttacgtgg
     2941 ttcgcgctgc cgtgtatgtg gcagttttac ggtctggcgg tcgcgcggca cgtctatctc
     3001 gcgccggacg aaaaatcgcc gcttttcgcc gccggaaccg aatggggcgg actttctttt
     3061 gccgtttaca acgtggtttg ttttctcgtg gcgtttctgc ttccgccgct tgccggcgcg
     3121 acaagccgca aaaccgtgca ttttatctgc ctgacgctcg gcgggctcgg attgctttcg
     3181 acttatttcg cgcacgacaa atactttttg tggctcggga tggcgggcgt cggcgtcgcc
     3241 tgggcttcta ttctctcgat gccgtacgtt cttctggcgg gcgcgattaa accttcgaga
     3301 atgggcgttt atatgggcgt tttcaatctg tttatcgtca ttccgcaaat cgtgatgtcg
     3361 ctcgtcgtgc cgcagcttta taaaaacgtt ctcggcgcag acccgcttaa cgtcgtgatg
     3421 ctcggcggtc tggtgatgct tctggcggcg gtcagcgttt tgctcgtcag ggatgtcggc
     3481 gcggcgaata tcgaaacttc cgttccgacg gcaagcggca ttgccggcgc ggagcgtgag
     3541 gatattaagg gctgaaaggc gagcaaatcg gctttccgaa aattaattta atcgaaacgg
     3601 gaatgttttt atgttgattt tgaaaaaggt gctcggcacg ctggctttaa cgataatttt
     3661 ctccaatttt atattcacgc agcaggcgca gcgcgatttc tcgaaagaaa acgcccgccc
     3721 gtcgcccgat tgggtgaaag acgccgtgat ttacgaaatt tttccgcgcg cattttctca
     3781 gaaaggcgat tttaattcga ttaccgctga tttggacaga ttaaaaaaca cgggcgtcac
     3841 cgttttgtgg ctgatgcccg ttcatccaat cggcagagaa aaagcgaaag gcacaatcgg
     3901 ctcgccctat gccgtaaagg attattacgc cgtcaatccc gattacggca cgagcgccga
     3961 tttgaaacgc ctcgtctcgg aagcgcaccg gcgcgggctg aaagtcatta tcgacatcgt
     4021 cgccaaccac accgcctggg acagcgtgat gatgaaaaat cccgcgtttt atacgaaaaa
     4081 cgagcgcggc gaaattatcc cgcccgtgcc cgattgggcg gacgtcgccg atttgaatta
     4141 cgacaacccg cagctccgca aatatattat cgagatgctg aagatgtggg ttcgcgatta
     4201 cgatttggac ggttttcgat gcgatgtcgc cggatttgtg ccgaccgatt tctgggaaac
     4261 ggcgcgcgcg gaagtcgata aaatcaagcc cgacacgatt tggctggcgg aatgggaatc
     4321 gccggatttg ctcgtcaaag ccttcgattt ggattattcg tgggcgaatc actcggcttt
     4381 gaccgaagtt ttgcagggca ataaaccggc ttcggaaatc cggcgcgtct gggaagagca
     4441 gaaagcgaaa tttccgaaaa acgctttgcg gatgcgcttt tccgataatc acgacgagcg
     4501 ccgcgccatc gcgcgcttcg gcgaaaaagg cgcgctggcg gcgcaggcgc tggtttttac
     4561 attggacggc gtgccgatgt tttataacgg aatggaagcg ggcgacacga ccgaatcggg
     4621 cgcgcccgca ttatttgaaa aactgccggt tttctggcgg atacaggagc ggcgaccgga
     4681 ttttccgaaa ttttacaaag cgatgaccga gcttcggcga aactcggtcg ctctaaggcg
     4741 cggcgattta gtctggctga aaaactcgga cgagacgaga gtcttaacgt ttttgcggcg
     4801 gcagggcgac gaagaaattc tggtggcgat caatatgaca aacgcgccgt ttttcggctc
     4861 ggtcgaagca ggcggaaatt ttgaagacgt gacgccgaat ctcgaaaaga aaaacgtcag
     4921 cctgcccgcc gtttcgctcg acgcgttcgg ttacaggatt tttagaaaga aaatttaaag
     4981 aaaacttttt aacgcgaaaa cgcaaaaaac gcgaccaaca aggacgttat gaaaaatcga
     5041 acgcggaaat ttattttatc caaaatctta agaaatcgtt ttaactttgc gcctttgccc
     5101 gtttgcgtgt tcgcgttaat ttttttctct tttacggctg cggctcaaac tctcgcgccg
     5161 ggcgcgcccg gcaaagacgc gcaatgggcg agcgccggta aacagggcgt cggaacttcg
     5221 gcgacactgg aaagtaaagt ctggtttaca ttgcagggcg gcgcgctgac ggaagtttat
     5281 tacccggacg taacggtggc gaacgttcat cttttgcagt ttgtcgtcgt caatccgaaa
     5341 acgcgtaaag tcgaaaccga gcgcgacgac gcgaatcatc aaattaaagt tttgcgtccc
     5401 gattcattaa ctttccggca aatcaacacg ggaaaatcgg gcgagtggaa aatcacgaaa
     5461 acctacacga ccgacgtcga gcgcgattcg gttttgattg acgtaaaatt cgagtcgaaa
     5521 aataaaaatt tgaatttata cgtgtattac gacccgtcgc tcggcaattc cggaatgcag
     5581 gatttcgcca gcccggcgct tggaacgggc ggcggcgcgg actcgaaact ggtcgcgcag
     5641 aacgaaaagc cgcccgtcat cgcttcggct ttatccttct ccggcgagat aagcgagcaa
     5701 acaagcggct tttatcaaat cagcgacggt ttagagcagc tcaggcaata cggaaaaatt
     5761 gttaagcggt atgacaaagc cggaacgggg aacgtcgttc aaatggcgag aattcttaag
     5821 ccttttcatt ttacgaccgt tctctcgttt gacccgaaca gctcggttaa agcatccgaa
     5881 aatagtatat attcgctcaa taagggtttc caaagatgcc tcgccgaata tgaaaaaggc
     5941 tggtctgatt acgtcaagac tctgcgccgg gtcgagccga aatatcaagc ccaattcaat
     6001 atggcggcga tgattttaaa ggcgcacgaa gataaagcga atcgcggcgc aaacatcgct
     6061 tctttgaccg ttccgtgggg cggcggcgaa aacgccaacg aaaacaacgt cggcggttat
     6121 catctggtct ggtcgcgcga tttgtatcag gtggcgacgg cttttatggc gctcggcgat
     6181 aaagcggcgg cggagcgcgc gctggatttt ctgttcaagg tccagcaaaa accggacggc
     6241 agctttccgc aaaattcctg gctcgacggc aagcctttct ggggttcgct gcagctggac
     6301 gaagtcgctt ttcctttaat tctcgcttat cagctcggca gattcgataa agcgacttat
     6361 acgaatcacg tcaaaaaatc cgccgacttt ttagtgaaaa ccggtccgcg aacgccgcag
     6421 gaacgctggg aagaagaagc cggattttcg ccgtcgacca tcgcggcgga aatcgccgga
     6481 ctggtctgcg cggcggaaat cgccagccga aacggcgacg aagcgagcgc gaccgtttat
     6541 ctggcgacgg ctgacgattg ggcgcgcaac gtcgagcgct ggacggcaac gacgaccggt
     6601 ttatacggcg acggcgttta ttatctgcga attacgcaga acggcgcgcc cgataaaggc
     6661 gagaaaatcg agctcaataa cggcgcggga ttttttgacg agcgcgcaat cgtcgacgcc
     6721 ggatttctcg aactggtgcg cctgggaatc aaatcggctg acgacccttt aattcgaaaa
     6781 tcggtgaagg tcattgacga ggttatcagg gtcaagacgc cgaacggcga cgcgttttat
     6841 cgttacaatc acgacggtta cggcgagatg gacgacgggc ggcgctggaa ctgggacggg
     6901 aaatataccg gcaaaggcag actctgggct ttgctttcgg gcgagcgcgg gcaatatgaa
     6961 ttggctttat gcggtcttca gaaatttata gttgacggaa cgggttgttc ggatttagtg
     7021 aagtatgagc gactcgaagc catgcaaaaa ttcgctaatg aaggcttgat gattcccgaa
     7081 caaatctggg acaaaaagga agcgccgaaa gccgattcgc agttttcgcc gaatctgaaa
     7141 ttcggcgagg gcacaggctc ggcgacgccg ctcgcctggt cgatggcgca gtttattcgc
     7201 cttgccgtca atttgcggga aggcagaaat cccgacacgc ccgacgttgt ttacaatcgc
     7261 tacgcccgca gcggaattcc ggcgcagacg agtaacttcg gcggcacgga cgaggaaatc
     7321 gttttgcccg taaacgccgg tcagaaattc agttttaagc gtcaagctat ggcgggcgcg
     7381 aaggttgcga tgtctttcag aggcgaaacg cggctgcttc caatcaacga aaaaggcgaa
     7441 atctcgtttg aaatcatcgc gcccgacgcg gaagaaatcg caattgtcgg catcatcacg
     7501 cctgacggcg cgtcggcgtt tgaaagagtt aagctgcgct cggcggatgc gaaaataaca
     7561 ggacagggaa actatgtttt ttcgccgccg cgcgagaatg agccgagtcc gggcattttc
     7621 ggtttaaaag gcgcggagaa aactgacgta atattcgttt acagaggcgc gggaaaaagc
     7681 gtcgagattg ccggagattt caccggctgg cgtccgcacg ggcggaaatt tcaaaatctc
     7741 ggaagctgtc aggttttgat tttgccgttt gataacgggg cgaagtttga atataagctc
     7801 gtcgttgacg gcaaatggat gaccgacccg ctgaatccgc ataaaatcga caacggcgtc
     7861 ggcggcgaaa attcggtttt cacgatgccc ggttataagc cgaccgtttg ggacaaagaa
     7921 caatctttca aaatggaagg cgttaatgaa aaattcataa tcgaaggcag gcacgaagca
     7981 tttgatttgc agacaaaaaa tttcggttcg cgccgtatca aagtttatct gccgataaga
     8041 tacaacgctt caaaagaccg ttatccggtt ttatattttc aggacggaag cgattacctg
     8101 caaagagcga aagccgccgt gcagcaggaa aacctggtcg gagcgggaaa aatcaagcct
     8161 tttataatgg tttttatcga cccgcgcgag cggacgaaag aatattgggc gaatgacgcc
     8221 tgggcggatt tcgtcgccaa tgaagtcgtt cccgaaatcg acaaaagata ccggacgatt
     8281 caaaatcgcg acgggcgcgc actgctcggc gcatcgctgg gcgggattac gagcgtctgg
     8341 atcggcttgc gtcatcccga caaatttgcg cgcatcggcg gtcaaagctc ttcgttctgg
     8401 g
//
LOCUS       DASWNE010000229         3070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_112835, whole genome shotgun sequence.
ACCESSION   DASWNE010000229 DASWNE010000000
VERSION     DASWNE010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112835"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..346
                     /locus_tag="VGB68_04660"
     CDS             185..346
                     /locus_tag="VGB68_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048552.1"
                     /translation="MEEILNLSPEKYAERFRRSAMKRTKISGLQRNARALKNNLAAEF
                     TVVEKTEEN"
     gene            457..2001
                     /locus_tag="VGB68_04665"
     CDS             457..2001
                     /locus_tag="VGB68_04665"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEY0048553.1"
                     /translation="MLRALQNQNIGIVIAFFIFFALCFADSAESAQTKNSAKTKATPA
                     KKNAKPTPTPSKKAQAKATPTPKNSKDKKGKTNSSASKSQPQTASKNKTADKSKQSSS
                     KSSAKTDQKTPGKQTKDSKTKDSKQNSNSKQNSNSRQNAKNTKSDSRQNAKNTKSDNS
                     KSDGSKNRADSNKSSSSRTSSGKTREKTFDRRNEPTRDTTKSNASTNRTPPKTSFEIS
                     TELPQIIVTDVSARVRSQAQANAPELSRLRLGTVLRVTEKNPAWYKVQYTSGGKTANG
                     WISANSVNDLNSGARLELYRQIVDRNYKTEMDFASASEMVDFLTQVSSELPSSNAAAE
                     LELKRLLALQTALKKIPSGQSERSPYREFLKAHEKSIIYSDPAAEWYVVSNLFWDLHK
                     KYAATAFADQIAWEGAENNLPGECEGYVNCYLFSERMTNGEYLRLHPNGKKAPQALAN
                     LTRFLSMVVEDLEKKTSYTGPTDVTDRADFYNLLAELRTIVSRLPLIEKEKTLQQLKQ
                     IAEAYR"
     gene            2132..>3070
                     /gene="moaA"
                     /locus_tag="VGB68_04670"
     CDS             2132..>3070
                     /gene="moaA"
                     /locus_tag="VGB68_04670"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEY0048554.1"
                     /translation="MLKDSYDRAIRDLRISLTDRCNFRCFYCLPDGEPPLARKDTILT
                     FEEIARLAEIFVALGIEKIRLTGGEPLLRRDVEALVEKIALLKSSDSENQNAGAQFEI
                     ANPKLVDLALTTNGHTLAKRAGNLKRAGLDRVTISLDSLKRENFQRITGVDKLEEVLT
                     AITAAQAAGLNPVKINAVIVRGYNDDEVVELAKFARSQSISMRFIEYMPLDSAHEWNR
                     GLVVSGREIREKINRVFPLRLKEASRGAETAWKYTFADGAAGEIGIIAPVTEMFCGAC
                     SRIRLTADGQIRTCLFSTAEYNLRDCLRSGAARAEIV"
BASE COUNT          887 a          786 c          751 g          646 t
ORIGIN      
        1 acgctgtttg tcttacgcta ctatcgaact tcgttcccct gaactccccg ctgaaattga
       61 aaaaaatcta aacggatggc tttacggctg cgatatttgt caggacgtct gcccgtggaa
      121 tcgttttgaa aagccgaccg aggaaacaag attcgagccg cgcgcgggaa atgtttccgc
      181 cgatttggaa gagattttaa atctttcacc ggaaaaatac gccgagcgtt tccgccgcag
      241 cgcaatgaag cggacaaaaa tttcggggct gcaacgcaat gctcgcgcat tgaaaaacaa
      301 tttagccgca gagttcacag tagtagagaa gacagaggaa aattaacaag ccagcgaaaa
      361 gtgaatagcg aaaaacgaaa aactgataac ttttcgtttt tcacttctcg ttttttattt
      421 tacctatcct gtttaaatct ttgtttggag agaaaaatgc ttagagcttt acaaaatcaa
      481 aatatcggaa tagtcatcgc tttttttatc tttttcgcct tgtgttttgc cgattcagcc
      541 gaaagcgcgc agaccaaaaa ctcggcgaaa acaaaagcga cgccggcaaa aaagaacgcc
      601 aagccgacgc cgacgccgag caaaaaggcg caggcgaaag caacgccgac gccgaaaaac
      661 tctaaggata aaaaaggcaa aacgaattct tctgcttcaa aatctcagcc gcaaaccgcg
      721 tcgaaaaata aaaccgcgga caaatccaaa caatcttcgt cgaagtcttc cgcgaaaacg
      781 gatcagaaaa ccccgggaaa acaaacgaaa gactcaaaaa ccaaggattc gaaacaaaac
      841 tcgaattcga aacaaaactc gaattccaga caaaacgcga agaatacgaa atccgattcc
      901 agacaaaacg caaaaaatac gaaatccgac aactcaaaat ccgacggctc aaaaaataga
      961 gcggattcaa acaaatcttc gtcttccaga acgagctcgg gaaaaacgcg cgaaaaaacc
     1021 tttgatagaa gaaatgagcc gacccgggac acgaccaaat caaacgcttc gacaaacaga
     1081 acgccgccga aaacaagctt tgaaatctcg accgagctgc cgcaaatcat cgtgacggac
     1141 gtttcggcgc gcgtccgcag ccaggcgcag gcaaatgcgc cggaactgag ccgcctccgc
     1201 ctcggcacgg ttctgcgcgt cacggaaaaa aatccggcgt ggtataaagt gcaatacacc
     1261 tcgggcggaa aaaccgccaa cggctggatt tccgccaact ccgtcaatga tttaaactcc
     1321 ggcgctcgcc ttgagcttta tcgccaaatc gttgaccgca attataaaac cgagatggat
     1381 tttgcctcgg cttcggaaat ggtcgatttt ttaacgcagg tcagcagcga gcttccaagc
     1441 tcgaacgcgg cggctgaact ggaattgaaa cgtctgctcg cgctccaaac cgctttgaaa
     1501 aaaattccgt ccggtcagag cgagcgctcg ccgtatcggg aatttctgaa agcgcacgaa
     1561 aaatcaatta tttacagcga cccggcggct gaatggtatg tcgtttcaaa tctgttctgg
     1621 gatttgcata aaaaatacgc ggcgacggcg tttgccgacc agatagcgtg ggaaggcgcg
     1681 gagaacaatt taccgggcga atgcgaaggc tacgtcaact gctatctttt ttccgagcgg
     1741 atgaccaacg gcgaatatct gcgcctgcac ccgaacggta aaaaagcgcc ccaggcgctc
     1801 gccaatctga cgagattttt atcgatggtc gtcgaggatt tggagaaaaa aacatcttac
     1861 acgggtccga ccgatgtgac cgaccgcgcc gacttttaca atctgctcgc cgagctgcgc
     1921 acaatcgttt cgcgcttgcc gcttatcgaa aaggaaaaaa cgcttcagca attgaaacaa
     1981 atcgccgaag cttaccgcta ggcgtcatta atgccaaacg caaaatagta agattcagga
     2041 ttcaagattc aggattaaat caattcttcc attttgcgtt ttgccttttc tgcgctttcc
     2101 aatccgcatt caaaaatcta aaatctaaaa tgtgttaaaa gattcttacg accgagctat
     2161 ccgcgacctt cgcatctcgc tcaccgaccg ctgtaatttc cgctgttttt actgtctgcc
     2221 ggacggcgag ccgccgctgg cgcgcaagga tacgattctg acttttgaag aaatcgctcg
     2281 tctggcggaa atctttgtcg cgctcggcat cgaaaaaatt cgcttgaccg gcggcgagcc
     2341 gcttttgcgc cgcgacgtcg aagcattggt tgaaaaaatt gcgctgctga aaagttccga
     2401 cagcgaaaac caaaacgcag gcgcgcaatt cgaaattgca aatccgaaat tggtcgattt
     2461 ggctttgacg accaacgggc atacgttggc gaaacgcgcc ggaaatctga aacgcgccgg
     2521 actcgaccgc gtgacgattt cgctggacag cctgaagcgc gaaaattttc agagaatcac
     2581 gggcgtcgat aaattggaag aagttttaac ggcgataacc gccgcgcagg ctgccggttt
     2641 aaatccggtc aaaatcaacg cggtcatcgt gcgcggctat aacgacgacg aagtggtcga
     2701 gttggcaaag tttgcgcgct cgcagtcaat ctcgatgcgc tttatcgaat atatgccgct
     2761 cgattcggcg cacgaatgga accgcggtct ggttgtttcc ggcagggaaa tccgcgaaaa
     2821 aatcaaccgg gtttttccgc ttcgtctgaa ggaagcttcg cgcggcgcgg aaacggcgtg
     2881 gaaatacacg tttgccgacg gcgcggcagg cgaaatcggc attatcgcgc cggtgacgga
     2941 aatgttctgc ggcgcctgct cgcggattcg gctgacggct gacgggcaaa tccgcacttg
     3001 tcttttttca accgccgaat acaatttgcg cgattgcttg cgaagcggcg cggcgcgcgc
     3061 ggaaatcgtc
//
LOCUS       DASWNE010000230         4488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_7164, whole genome shotgun sequence.
ACCESSION   DASWNE010000230 DASWNE010000000
VERSION     DASWNE010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7164"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1883
                     /locus_tag="VGB68_04675"
     CDS             <1..1883
                     /locus_tag="VGB68_04675"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEY0048555.1"
                     /translation="YSAHDILRGVSFQINQSEKVGLVGRNGAGKTTVFRLITGAETGD
                     AGEVVKINNLKLGLLEQHVDFTESETVHTAALSAFQKLHDIEAEMRQLEIQMAENPSD
                     EILEQYAELQNEFEREDGFTYTARAESILLGLGFAKETWATATKNLSGGQKNRLGLAR
                     LLLSQPDVLLLDEPTNHLDVNAVEWLENFLQSYEKTFVLISHDRYFLDRVCNRIIEIE
                     SGKAVSYKGNYTDFLVEREERRAAQQRAFENQQAFIAKNEDFVRRNIAGQKTKMAKSR
                     RNMLERLERVDAVSSDRASGNFQLKKVERTGNQVLTVESLSIGYGEKILAKNIDFSLL
                     RGEALGVIGGNGTGKTTFLKTILGNQREISGKILWGAKTDTGYYSQNLEDLEERNEII
                     QELRRVAPLAENGELRGFLARFLFTGEDIFKQVKDLSGGEKGRLALAKLIYSNKNVLV
                     LDEPTNHLDIPSREALEAALDAFQGTIITVSHDRYFLDRIATQILSFETDKTVEIFKG
                     NYSEFHDWKSSQPPAGSRQSEKSNSPATDENNEEQKSKTKDQLSKNQRQRVENRIREI
                     EKQIPEAEERLAQLSLQMSQPEIASNHARLQEVSAQFQQSERKIQSLYEEWDELLGSL
                     SD"
     gene            complement(1869..2420)
                     /locus_tag="VGB68_04680"
     CDS             complement(1869..2420)
                     /locus_tag="VGB68_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048556.1"
                     /translation="MLRIAFAFSCIFLFAFPALGQESENLPLKLTVSLKKDSFCLNQA
                     IPVKAVLENITTDKIAIDKNSLWYTHSSQYTVSEVILPKNRRRRGSGSGVASTSISTV
                     IGHHGPDYVGDYLILSAGQKFEDTSSFAGSVYFNKTGIYTFELTYGQFLRDSFEEKEV
                     WRGAVRSNKVTFEIVACKASVRK"
     gene            complement(2476..3945)
                     /gene="guaB"
                     /locus_tag="VGB68_04685"
     CDS             complement(2476..3945)
                     /gene="guaB"
                     /locus_tag="VGB68_04685"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEY0048557.1"
                     /translation="MLINEIKEGLTFDDVLLVPAYSEILPNEVDTSTQFSRNIRLNIP
                     LCSSAMDTVTEASLAIALAQQGGIGVVHKNLSIERQAEEVDKVKRSESGMIVDPVTIG
                     DNALVSEALNLMERFRISGIPVIDENGFLVGIITNRDLRFETRFDIPVSEVMTKQPLV
                     TVPVGTTLDGAKGKLQQHRIEKLLVVDDNGLLKGLITVKDIQKAIRYPHAAKDNLGRL
                     RVAAAIGATGDFFERAEALVDSRADAIIIDTAHGHSSRVIEAVKKIRNRFGDIELIAG
                     NVATAEATKALIEAGVDAVKVGIGPGSICTTRVVTGAGVPQITAITECVSAAKGTGVP
                     VIADGGIKFSGDVAKAIAAGADSVMIGSLFAGTEEAPGETILFQGRTFKTYRGMGSIG
                     AMKKGSSDRYSQEGTVADSKYVPEGIEGRVAYKGTLADMVTQLVGGLRSGMGYTGCKS
                     IAELQENAGFVRITSAGLRESHVHDVIITKEAPNYSLES"
     gene            complement(4152..>4488)
                     /locus_tag="VGB68_04690"
     CDS             complement(4152..>4488)
                     /locus_tag="VGB68_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048558.1"
                     /translation="NVNTNTSPVPSTNEINVEFKTSETVAVTYGTDGKTANRNVTSGA
                     PLVIKAQQNVRLRYYRGFADKVQLTINGQQVKPPAPPAKGAGIEFEITKDNIAQILQS
                     GAVTLAAPQ"
BASE COUNT         1102 a         1170 c         1171 g         1045 t
ORIGIN      
        1 cctacagcgc gcacgacatc ttgcgcggcg tttcgtttca gataaaccag agcgaaaaag
       61 tcgggctggt cgggcgcaac ggcgcgggca aaacgaccgt ttttcgattg attacgggcg
      121 cggaaaccgg cgacgcgggc gaagtcgtta aaatcaataa tttaaagctc gggctgctcg
      181 aacaacacgt cgattttacc gaaagcgaaa cggttcacac ggcggcttta tccgcttttc
      241 agaaactgca cgacatcgaa gccgaaatgc gccaactgga aattcagatg gcggaaaatc
      301 cgtcggacga aattctggag caatacgccg agctgcaaaa cgaattcgag cgcgaagacg
      361 gttttaccta tacggcgcga gccgaatcga tcctgctcgg tctgggcttt gccaaagaaa
      421 cgtgggcgac cgccacgaaa aatctttccg gcgggcagaa aaatcgactc ggtttggcgc
      481 ggcttctgct ttctcagccg gatgttttac tgctcgacga gccgaccaat catctggacg
      541 tgaacgccgt cgaatggctc gaaaattttc tgcaatctta cgaaaaaact tttgttctca
      601 tcagccacga ccgttatttt ctcgatcgcg tctgcaaccg catcatcgaa atcgaaagcg
      661 ggaaagccgt ttcctacaag ggcaattaca cggatttcct cgtcgagcgc gaagaacgcc
      721 gcgcggcgca gcagcgcgct tttgaaaacc agcaggcttt cattgccaaa aacgaagatt
      781 tcgtccggcg caacatcgcc ggacagaaaa cgaaaatggc gaaatcgcgc cgcaatatgc
      841 tggaaaggct ggaaagagtc gatgcggttt cgtccgaccg ggcgagcgga aattttcaat
      901 tgaaaaaagt cgagcgcacg ggaaatcagg ttttgacggt cgaaagctta tcaatcggct
      961 acggcgaaaa gattctggcg aagaacatcg atttttcgct tctgcgcggc gaggcgctcg
     1021 gcgttatcgg cggcaacgga accggaaaaa ccacgtttct gaaaacgatt ctcggaaacc
     1081 agcgcgagat ttcgggaaaa attctctggg gcgcaaaaac cgatacgggt tattattcgc
     1141 agaatctcga agatttggaa gaacgcaacg agattataca ggaattgcgc cgcgtcgcgc
     1201 ctctagccga aaacggcgaa ttacgcggat ttctggcgag atttcttttt acgggcgaag
     1261 acatttttaa acaggtcaaa gatttgtccg gcggcgaaaa gggaagactg gcgctggcga
     1321 aactgattta ttcaaacaaa aacgtcctgg tgctggacga accgaccaat catctcgaca
     1381 ttccctcgcg cgaagcgctc gaagcggcgc tcgacgcttt tcagggaacg attatcaccg
     1441 tcagccacga ccgttatttt ctggatagaa tcgccacgca gattttgtcg tttgaaacgg
     1501 ataaaacggt tgagattttc aaaggcaatt acagcgagtt tcacgattgg aaaagcagcc
     1561 agccgccagc cggcagccgc cagtcggaaa agagcaattc gccggcgacg gacgaaaata
     1621 acgaagagca aaaatcgaaa accaaagacc aattatcaaa aaaccagcgc cagagagtcg
     1681 aaaatcgcat cagggaaatc gaaaaacaga ttcccgaagc cgaagagcgc ctcgcgcagc
     1741 tttctcttca gatgagccag ccggaaattg cttcaaatca cgcccgcttg caggaagttt
     1801 cggcgcagtt tcagcagagc gaacgaaaaa tccaatcctt gtatgaagaa tgggacgagc
     1861 tgctcggctc actttcggac tgaagctttg caggcgacga tttcaaaagt tactttattc
     1921 gacctgacag cgccgcgcca gacttctttt tcttcaaagc tgtcgcgcaa aaactgaccg
     1981 taagttaatt caaaagtgta aattccggtt ttgttaaaat agaccgaacc ggcgaaagag
     2041 ctggtatctt caaatttttg accggcggat aaaattaaat aatccccgac ataatcggga
     2101 ccgtggtgac cgatgacggt cgaaatagaa gtcgaagcaa cgcccgaacc gctgccgcgc
     2161 ctccgacggt ttttcggcaa aatcacttcc gaaacggtat attgcgagct gtgcgtatac
     2221 cagagcgaat ttttgtcgat ggctatttta tccgtcgtga tgttttccag tacggctttt
     2281 accggaatcg cctgattcag gcagaatgaa tcttttttaa gactgacggt cagtttcagc
     2341 ggcagatttt cgctttcctg accaagcgcc gggaaagcaa aaagaaaaat acaggaaaaa
     2401 gcaaaagcaa ttctcaacat acttttaaaa aagatgtccg gcgaattgct tttgttgcag
     2461 tatcggaaaa tatatctaac tttccaggct gtaattcggc gcttctttcg tgataatcac
     2521 gtcgtggacg tgcgattcgc gcaagcccgc cgaggtgatg cgaacgaatc cggcgttttc
     2581 ctgtagctcc gcgattgatt tgcagcccgt gtagcccatt cccgaacgca gaccgccgac
     2641 gagctgcgtg accatatccg cgagcgttcc tttataagcg acgcgacctt cgatgccttc
     2701 gggcacgtat tttgaatcgg cgaccgtgcc ttcctgcgag tatctgtcgg aagagccttt
     2761 tttcatcgcg ccgatggagc ccattccgcg ataagtttta aacgtgcgtc cctgaaaaag
     2821 aatcgtctcg cccggcgctt cttccgttcc ggcaaacagc gagccgatca tcacgctgtc
     2881 cgcgccggct gcaatcgctt tggcgacgtc gccggaaaat ttaatgccgc cgtcggcgat
     2941 aaccggaacg ccggttcctt tcgccgcgct gacgcattcg gtaatcgccg taatctgcgg
     3001 aacgcccgcg ccggtaacga cgcgcgtggt gcaaatcgag ccgggaccga taccgacctt
     3061 gacggcgtcg acgccggctt caatcaatgc tttcgtcgct tcagccgtcg ccacgttgcc
     3121 ggcaatcaat tcgatgtcgc cgaaccgatt gcggattttt ttgacggctt cgataacgcg
     3181 cgaagaatgt ccgtgcgccg tatcgatgat gatggcgtcg gcgcgcgaat cgactagcgc
     3241 ttcggcgcgc tcgaaaaaat ctccggtcgc cccgattgcc gccgccacgc gcaggcgtcc
     3301 gagattatct tttgcggcgt gcgggtagcg aatcgctttt tgaatgtcct tgaccgtgat
     3361 taagcctttt aaaagcccgt tgtcgtcgac gaccagcaat ttttcgatgc gatgctgctg
     3421 gagtttccct ttcgcgccgt caagggtcgt gccgaccgga acggtgacga gcggctgttt
     3481 ggtcatcact tcggaaacgg gaatgtcaaa gcgcgtttcg aaacgcaaat cgcgattggt
     3541 gatgatgccg accagaaaac cgttttcgtc gatgacggga atgccgctga tgcggaagcg
     3601 ttccataagg ttcagcgctt ccgagacgag tgcgttgtcg ccgatcgtca ccgggtcgac
     3661 aatcatcccc gattccgagc gcttcacttt atcgacttct tccgcctgcc gctcgattga
     3721 taaattttta tgaacgacgc cgattccgcc ctgctgcgcg agcgcgattg ccaggctcgc
     3781 ttccgtcacc gtatccatcg ccgacgagca gagcgggatg ttcagtcgaa tgtttctgga
     3841 aaactgcgtc gaggtatcga cttcgttcgg cagaatttcc gaatacgccg gaaccagcag
     3901 aacgtcgtca aatgtcaagc cttctttgat ttcatttatt aacataaatt cgttagttgt
     3961 cagttgtcat ttgccggttg tcagtcgccg ccgggaataa accgatttct cagccgtttt
     4021 cttaatctgc ggctgaccgc cgaaaagtga ctatctataa aataaaaccg cccgcacttt
     4081 tcaggcgggc gctggctcaa gctaaccgac atttaaaaaa atgagttttc gtttgtaaaa
     4141 aagtcgcata actattgcgg cgcggcgagc gtcacggcgc cgctttgcag aatctgcgcg
     4201 atattatcct ttgtaatctc aaattcgatt ccggctccct tggcgggcgg cgcgggcggc
     4261 ttaacctgct gaccgttgat tgtcaactga actttgtcgg cgaagcccct gtaatatctc
     4321 agtcttacat tctgctgagc cttgatgacg agcggcgcgc cgctcgtcac gtttctgttt
     4381 gccgttttgc cgtcggttcc gtaagttacg gcaaccgttt cgctggtttt gaattcgacg
     4441 ttgatttcgt tggtcgacgg aaccggcgac gtgtttgtat taacgttt
//
LOCUS       DASWNE010000231         1975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_147749, whole genome shotgun sequence.
ACCESSION   DASWNE010000231 DASWNE010000000
VERSION     DASWNE010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147749"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..403
                     /locus_tag="VGB68_04695"
     CDS             191..403
                     /locus_tag="VGB68_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048559.1"
                     /translation="MRFPVRDKCCAIESVSCITFEPPTRMCTRASFSRLREKNLVQSS
                     LTLTAAPTAWRASTNNSSLPSRLIAA"
     gene            complement(389..565)
                     /locus_tag="VGB68_04700"
     CDS             complement(389..565)
                     /locus_tag="VGB68_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048560.1"
                     /translation="MSLPEIKREILPGGDVRLSSGDCTFVYRRPRAGALLVTIWLRRR
                     AIRDDDARRNSRGD"
     gene            complement(562..1854)
                     /gene="eno"
                     /locus_tag="VGB68_04705"
     CDS             complement(562..1854)
                     /gene="eno"
                     /locus_tag="VGB68_04705"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016387099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEY0048561.1"
                     /translation="MSLIEQVWAREILDSRGNPTLEAEVVLESGETGRAAVPSGASTG
                     ENEAVELRDGDAARFLGKGVLNAVENVNEKIAYELEGLDALDQTLIDEALLALDGTDN
                     KSNLGANALLAVSMATARAAAAFLEVPLYRYLGGVNARTLPVPMMNIINGGAHADNNV
                     DFQEFMIMPVGAETFSEGLRAGAEIFHNLKKVLSSRGYLTTVGDEGGFAPNLKSNEEA
                     IETILEAIEKAGYAAGENILLALDPAASEFYDGTNYVFKKSDKRVLSSDEMVAYWADW
                     CAKYPIISIEDGMGESDWDGWKNITGQLGKKIQLVGDDLFVTNTKFLQKGIDTGAANS
                     ILIKVNQIGTLTETLDAIELAKTNNMTAVISHRSGETEDSFIADLAVATNAGQIKTGS
                     LSRSDRIAKYNQLLRIEEDLGSAARYLGRKAFYQIKAG"
BASE COUNT          421 a          571 c          500 g          483 t
ORIGIN      
        1 gcgaaatttt ccggcgaatg cctgccgcga ttaaaaagcg tgaccgcgtg acctctgtcc
       61 cgcgccgccg cgatcaggtg gcgaccttaa aattttgttc cgccgatgat tagaattttc
      121 atttcaaatc cggtaagttt aattattgtt taatttgccg tcgaacagct ccgcgtcgga
      181 ataaatctga atgaggtttc cggtgcgcga caaatgctgc gcgatagaaa gcgtcagctg
      241 cataactttc gagccgccga ccagaatgtg cacgcgcgcc agcttttcgc ggctcagaga
      301 gaaaaatctc gtccagtcgt cgctgacgct gaccgccgcg ccgaccgcct ggcgggcatc
      361 gacaaataat tcgagcctgc cttcgcgatt aatcgccgcg tgaatttcgt cgagcgtcgt
      421 cgtcccgaat tgcccggcgt cgtagccaga tagtaacgag cagcgcgccg gcgcgcggtc
      481 ttcgatagac gaacgtgcag tcgccggacg acaggcgaac gtcgccgccc ggcaaaattt
      541 ctcttttgat ttccggcaag ctcatcccgc ttttatctga taaaacgctt tacgtcctaa
      601 ataccgcgcc gcgctgccca aatcttcttc gatgcgcaaa agctggttgt atttcgcgat
      661 gcggtcgctg cgcgaaagcg agccggtttt gatttgtccg gcgttggtcg ccaccgctaa
      721 atccgcgata aacgaatctt ccgtctcgcc cgaacgatgc gagataacgg cggtcatatt
      781 attggtttta gccagctcga tggcgtccag agtttcggtc agagtgccga tttgattgac
      841 ttttatcaaa atcgaatttg ccgcgcccgt gtcgattccc ttttgcagaa atttcgtgtt
      901 tgtgacgaac aaatcatcgc cgacgagctg aatttttttg ccgagctgcc cggtgatatt
      961 tttccagccg tcccagtccg attcgcccat tccgtcttcg atggagataa tcggatattt
     1021 cgcgcaccaa tccgcccagt aggcgaccat ttcatcgctc gacaaaacgc gtttgtccga
     1081 ctttttaaaa acgtaattcg tgccgtcgta aaattcgctc gccgccgggt cgagcgccag
     1141 cagaatgttt tcgcccgccg cgtaaccggc tttttcgatt gcctccagaa tcgtttcaat
     1201 cgcttcctcg ttcgatttca gattcggcgc aaagccgcct tcgtcgccga ccgtggtcag
     1261 ataaccgcgc gagcttaaaa cttttttgag attgtgaaaa atctcggcgc ccgcgcgcaa
     1321 gccttcagaa aacgtttcgg cgccgacggg cataatcata aattcctgaa aatccacgtt
     1381 gttgtcggcg tgcgcgccgc cgttgatgat gttcatcatc ggcacgggca gcgttcgggc
     1441 gttgacgccg ccgagatagc ggtaaagcgg cacttccaga aacgccgccg ccgcgcgcgc
     1501 cgtcgccatc gaaaccgcca gcagcgcgtt tgcgccgaga ttcgatttgt tgtccgtgcc
     1561 gtcgagcgcc agcagcgctt cgtcaatcaa agtttggtcg agcgcgtcca agccttccag
     1621 ctcgtacgcg attttttcgt tgacgttttc gaccgcgttt agaacgcctt tgccaaggaa
     1681 tcgcgccgcg tcgccgtcgc gcagctcgac cgcttcgttt tcgccggtcg aagcgcccga
     1741 aggcaccgcc gcgcgccccg tctcgcccga ttccaaaaca acttccgctt ccagcgtcgg
     1801 attgccgcgc gaatccagaa tttcccgcgc ccagacttgt tcgattagac tcattttgat
     1861 aaaagactcc gttaaaaaaa ttagataaag ctagtttgat aaatttaacg tgaatcggaa
     1921 ggaaaaacaa agcagtgatt attaatccat tttctcgtct acttagacgg caagc
//
LOCUS       DASWNE010000232        13917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_466398, whole genome shotgun sequence.
ACCESSION   DASWNE010000232 DASWNE010000000
VERSION     DASWNE010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13917
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466398"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VGB68_04710"
     CDS             complement(<1..269)
                     /locus_tag="VGB68_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048562.1"
                     /translation="MKQPEKTETQVRVESSTSTVPAFETSPRPTPSPAVETTAPKVQE
                     TTQAKVEPSPSSLRVVVTSTLPLPEATPAPTVEATPPTPTPTPKP"
     gene            complement(339..2258)
                     /locus_tag="VGB68_04715"
     CDS             complement(339..2258)
                     /locus_tag="VGB68_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048563.1"
                     /translation="MKSFYLLLSIILLLSPAAFAQKPKARPTTVKKPPAVKSVNDAEE
                     FEKAKAVAAAPQRIEALQKFLRDFPQSEQKNRALELIVGARAEIAEQKLTLSETEEGV
                     RLFILAVAEAPKPMSEQLFATVMMQLPTNLFYRGQPKAAVEVAKLIEEKIEGNSKQLL
                     ALGAFYLGIESADDAKRLAEKVLAVDAATLTVESQISAYQMLGMANRLNFDLEASANA
                     YSKALELNPGSIVSKRSLAEMKRAIGKTDEAAALYREILEKDNADNTAQTGLVLSLFD
                     SGKQPEAETELAKSLEANPKNLFLLVGAAYWYAAHNQAGKAVDYAQRAVDVEPRYTWG
                     QIALARALMLQKKPLEAEKALLTAQQYGNFPTLDYELASVRLAAGFYEEAAAGIRKRF
                     IVDEGVIYTRLGNRVLKESKNFIDLLAPERRASIFESAGSDSPETAEKLKSLLYLYQK
                     LNDKEPSETQITEAADEFVKGEDNAKTYRQIYVASRLLQKKIALPKVLELTQGAVRGV
                     DTSLDVDSPSSAVLADELFDTRNLAMQRGQLVIVPNLPRQTLSNILRGRIEEIAGWTL
                     YQQEKPQEAGIRLKRAVGILPEKSAWWRSSQWRLGASLEAGGKPTEALEAYIKSYVNG
                     EPDASKRLVIEALYQ"
     gene            2399..2920
                     /locus_tag="VGB68_04720"
     CDS             2399..2920
                     /locus_tag="VGB68_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048564.1"
                     /translation="MMNELNEFWTNSINEAIDKARAEGRGDVADYLTLKATNDEIRAA
                     SCRWLFESLLEIAHETMRGGLNLKIENENPYRFEMNKATMVGSLMEFKFGIRRLSAEA
                     GWTRTPQDGFMRGGALAFARLAHFGQAKHNAELILIRSADSAPQWFAVDRAGRQTPFD
                     SNNLREHFRIFLG"
     gene            3026..3760
                     /locus_tag="VGB68_04725"
     CDS             3026..3760
                     /locus_tag="VGB68_04725"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HEY0048565.1"
                     /translation="MKSELEKLIELQKTDTNIRKLKKAIETSDERRAAIEREFEQHAS
                     SIREIQTSRDGARSERERLEAQIAENKTYLERADRNLKHAQNQKEYETAMRETDALQK
                     QIAALEAQVMEKMTTLEEVEKVLGERSEEISSLESNRQGELETFETELAANRAELDGE
                     MKKRHEVFVTLPANLASVYNRMAQRSRDGIAVAEVKNGSCSACFMKLRPQMQVEIKKG
                     DRIITCESCARILYITPEAKTATQES"
     gene            complement(4022..5797)
                     /locus_tag="VGB68_04730"
     CDS             complement(4022..5797)
                     /locus_tag="VGB68_04730"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEY0048566.1"
                     /translation="MEIYFQVITEAEPLREACEKLSAADYLGFDTETTELDPYQGIVR
                     LVQLSDGKDTSVIDLKAFADRGDVKTTPELEPLRRLLSATKPIKVAHNAKFDAKWIKH
                     YLGVDINGIFDTLLASQLIAAGDQDRRHNLAEVSSYFLGVELDKSEQVSDWNAPELSP
                     AQIEYAARDAATMVPLREKIVERLKTDDLIKVAKLEFDCVMPVAAMELNGFYLDEARW
                     REQLEKVKKEQAVVALELQQMLSAGVAQASLFGAAEINLDSQAQVTDALKNLGVPVPA
                     TTRGWQLQPLAADYPVVAKLLEYRSVAKSLTSFGENILEFIKPQTGRIHADFRQIGAP
                     TGRFSCNNPNLQQIPHAEEYRRCFRAPEGKKLVIADYSQVELRILADFSNDENFIKAF
                     QSGADFHTITGAQVFNVKPEDVTPDQRSFAKRLNFGVVYGIGASRFAMMTGLPQTEAE
                     NIMRRYFATYRGLDAWLRDAARKVTAERLARTATGRMMRFRFDENDHAQIAAAQRNGK
                     NMPIQGTSSDILKRALRLLHDKFNGTSARLVNIVHDEVIVECDAPEAETTQKNLEDAM
                     CAAGEEYIKKVPVKVDSKIADEWAK"
     gene            5980..6195
                     /locus_tag="VGB68_04735"
     CDS             5980..6195
                     /locus_tag="VGB68_04735"
                     /inference="COORDINATES: protein motif:HMM:NF044773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048567.1"
                     /translation="MPTASCPECSEKVYVDADSEQGDLVSCDECGSDLEVVGLDPIEL
                     DLYEDKGEKDDYDEVDFDSLDYQNERF"
     gene            complement(6307..7299)
                     /locus_tag="VGB68_04740"
     CDS             complement(6307..7299)
                     /locus_tag="VGB68_04740"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0048568.1"
                     /translation="MLFRIQNLRFGRFSLSRFFRVGILLLLAAGGWLPPVRAQDDVIR
                     VETDVVAFEVSVTDKDGKPVRGLEAGDFKVFENGVERKVDFFEPLRKSDESRPLSIVF
                     ALDVSGSMTRDELIRLRDAMQKFIERLADYQSYFAVITFGMEVKTLQSFTNKREKLEK
                     TFEKILKDQDGLSTHAYDATDDAIRLLRKKAPDARNNKLMKRAVILITDGFPVGDTVS
                     PKTVIERANNAETSVYSVILPSFSRLQGTKKPLLTPLEASGLIEKTGGKSFYATQTDF
                     EPLFKALAEEITASYVLAFYPSEEDKTNGKFREIRIESTKGYQVKQNRAGYRLK"
     gene            7645..10227
                     /locus_tag="VGB68_04745"
     CDS             7645..10227
                     /locus_tag="VGB68_04745"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY0048569.1"
                     /translation="MHKNLDLEVKIVKQFDISEYSSPTKILGGVLLALSAAVLVYTIY
                     GCFSFTFLQFVILGVSMTVAALANQYQFKIPKTRLTISPRKVFIFWGTIWLGSAGGVL
                     LSLISSIVRYRTTKNKIKWVYGIFIHTVATFAAAEVFYLFLHQFAGFDGWNVAANSIN
                     LGWLLLAAALMTLTHYVLTTLLSSLLLAFDSEDGFRSIWKKRYTRTIANYLVAFAAML
                     VLHLSFRHFGVDFGLVVLPTAIFGHLAYKIHLQRLAQKTREITEASRIHLATVEALAT
                     AIDARDQVGIGHVRRTQIYAVGIGEILGLSEKEINALRTGALLHDIGKLAVPDHILNK
                     PGRLTPAEMEKMKIHASVGASILEDVKFDCPVVPTVKYHHEAWDGSGYPEGLKKENIP
                     LTARILSIADAYDSLRGARPFRGAISRDEARKSLLNAAGAKFDPKLVDIFLRNLQHFE
                     AEIESQGLSYHFDGAEASIFGIEAGEANENYVDQIKRANREVFTLYEMAKVFSSSLNL
                     PDTISRFTAKIGELVPFDTCVVYLLDEAQDAATAVYAEGKNSAALKGKRVKSGEGATG
                     YALKKRQPVYNINPALDFSFEMHDFILEYTAMASLPLMAGERLIGAVSLYSCELENYE
                     DEHMRLLETISRIAADAISKSLHLAETESRALTDPMTGLPNARSLQAQFENEIARARR
                     NGSEFQVLMLDLDGFKLVNDTFGHKVGDVLLREISKVMRGQLRDYDFLARYAGDEFVI
                     IAPEMNNEGVSELCQRMEKAVADFVLPIGDGRFAQVGVSLGAAAYPRAGDTLDQIIVA
                     ADQAMYAVKAARKRKQNQPPVQTESGAQPLLPEAHEFPHMLIAEEALIVELDESHIVS
                     AAIN"
     gene            complement(10340..11416)
                     /locus_tag="VGB68_04750"
     CDS             complement(10340..11416)
                     /locus_tag="VGB68_04750"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF006707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEY0048570.1"
                     /translation="MAVDYSKIQELLGADADTLLNHTSKTIPKETLNLPGGDFVDRVW
                     TLSDRTPATLRALQTLFDNGRLRGTGYLSILPVDQGIEHSAGASFAPNPQYFDPENIV
                     KLAIEGGCNAVASTFGVLGSVARKYAHKIPFVVKINHNELLTYPNKFDQVMFGAIDQC
                     RDMGAVAVGATVYFGSDESTRQLGEVAEAFAYAHELGMATILWCYLRNNKFKTKEGDL
                     HTAADLTGQANHLGVTIQADIIKQKLPERNGGYNVLGLESSYGKTNPKIYTDLTSDNP
                     IDLVRYQVANCYMGRCGLINSGGESKGAGDLADAVRTAVVNKRGGGTGLISGRKAFQR
                     PMAEGVELLNAIQDVYLSEEITVA"
     gene            11676..13706
                     /locus_tag="VGB68_04755"
     CDS             11676..13706
                     /locus_tag="VGB68_04755"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 and transglutaminase domain-containing
                     protein"
                     /protein_id="HEY0048571.1"
                     /translation="MLSFVQRSALLLLLMYLVCLSAESAEAQTAEWRPVSPAELEMKT
                     PQVEPDADAEAIFWEVRLDDKKSNKLSYNHYVRVKIFTERGREKFSKFDIPFYKGRKV
                     EDVAARVIKPDGTTIDVRPADIFEREIIKAGKIKIRAKSFAVAGIEPGVIVEYRYVEV
                     VKNDSLNNERLIFQRDIPMQRATYYVRPYQGTILSFDFRNMQAVRFVESSDGFFVGTA
                     ANVPALKEEAYMPPEDEVRRWARLYYNGYNSFSWNTLSYQVTQTFNKIIKPTKELNQK
                     AAELTAGATTDEEKLRRIYDFTQKRIKNVNFDSSYTEEQLESIKIKDADDVLKRGVAN
                     SVFVNWLFGALAKAAGFEVNVVLAADRSEHFFNPNDNISPGYLHPAGIAVRMKDQEKP
                     QQIKPVTGGAHASSGRSTAGQTWKFFDPGTLYLPFGRMVWNEEDVYALLVGESSSQWR
                     KIPLSGVGLSPARRAGKFKLAEDGTLEGSVRLEYEGHQAISRRRAEFKDSPAKREENL
                     KEEIKKRISAAEISDVTIENFDNPALPLTYNFKIRVPNYAQKTGKRLFFQPGFFEYGT
                     TPVFSSATRTHSVYFQYPWSEQDDVEFELPKDFSLDNAEAPADVADSQQIGSLKINIG
                     IDKVNGKIIYKRSFYFGNSGLLLFPVSKYEPLKSLFDSFHKADTHTITLKQN"
BASE COUNT         3225 a         3574 c         3732 g         3386 t
ORIGIN      
        1 tccggtttcg gagtcggcgt cggagtcggc ggagtagctt ctacggtcgg cgcgggcgtg
       61 gcttcaggca gcggcagagt tgacgtgacg acgacgcgca gcgacgacgg gctcggttct
      121 actttcgcct gtgttgtttc ctgaactttc ggagctgttg tttcaactgc cggcgaaggc
      181 gtcgggcgcg gcgaagtttc aaatgccgga accgtcgaag tcgaagattc gaccctgact
      241 tgcgtctcgg tcttttccgg ctgtttcatt aaaaaactga cggaaggctt cggtttggcg
      301 ccgattttct cgtccagtcc atcggtcgtg ccgttgattt attgataaag cgcttcgata
      361 acgaggcgtt ttgacgcgtc cggctcgccg ttgacgtaac tttttatata agcttcgagc
      421 gcttcggtcg gcttgccgcc ggcttcgagc gatgcgccca gtcgccactg gctcgaacgc
      481 caccaggcgc ttttttccgg cagaatgccg acggcgcgtt tcaggcggat tccggcttcc
      541 tgcggttttt cctgttgata aagcgtccag ccggcgattt cctcgatgcg cccgcgcagg
      601 atattggaaa gcgtctggcg cggcagattc ggcacgataa ccagttgacc gcgctgcatc
      661 gcgagatttc gggtgtcgaa aagctcgtcc gccagaaccg ccgacgaagg cgaatcgacg
      721 tcgagcgaag tatcgacgcc gcgcaccgcg ccctgcgtca gctccagaac tttcggcagc
      781 gcgatttttt tctgcaaaag acggctcgcg acgtaaatct gccggtaggt tttcgcattg
      841 tcttcgcctt ttacgaattc gtcggcggct tccgtaattt gcgtttcgct cggttccttg
      901 tcgttgagct tctggtaaag atacaaaagg cttttcagct tttcggcggt ttcgggactg
      961 tcgctgccgg cggattcaaa aatactggcg cggcgttcgg gcgcgagcaa atcgataaaa
     1021 tttttcgatt cttttaaaac ccggttgccc agccgcgtat aaattacgcc ttcgtcgacg
     1081 ataaatcttt ttcgaattcc ggcggcggct tcctcgtaaa atccagccgc gagccggacg
     1141 gacgccagtt cgtaatcgag cgtcgggaaa tttccgtatt gctgggcggt gagcagcgct
     1201 ttttccgctt cgagcggttt cttttgcagc atcagggcgc gggcgagcgc gatttgtccc
     1261 cacgtgtagc gcggctcgac gtcgacggct ctctgcgcat aatcgacggc tttgcccgcc
     1321 tgattgtgcg ccgcgtacca gtaagccgcg ccgaccagca gaaacagatt tttcggattc
     1381 gcttcgagcg atttggcgag ctccgtttca gcttcgggct gttttccgga gtcgaaaagc
     1441 gacaaaacca agccggtttg cgccgtgtta tcggcgttgt ctttttctaa aatttcgcgg
     1501 tagagcgcgg cggcttcgtc ggttttgccg atggcgcgtt tcatctccgc cagcgagcgt
     1561 ttcgagacaa tcgagcccgg attcagttcc agcgctttgg agtaagcgtt tgccgaagct
     1621 tccaaatcga aattcaggcg attcgccatc cccagcattt gataggcgga gatttgtgat
     1681 tcgaccgtca gggtcgcggc atcgaccgcc agaacctttt ccgctaatcg tttggcgtcg
     1741 tcggcgcttt cgatacccag ataaaatgct ccgagagcca gcaattgttt ggagtttccc
     1801 tcgatttttt cctcgataag ctttgcgact tcgaccgcgg cttttggctg accccgataa
     1861 aaaagattgg tcggaagctg catcatcacc gtcgcaaaaa gctgttccga catcggttta
     1921 ggcgcttccg cgacggcgag gatgaaaagc ctgacgcctt cttcggtttc gctcaaagtc
     1981 aatttttgct cggcgatttc ggcgcgcgcg ccgacgatta attccagcgc gcggtttttc
     2041 tgttcggatt gcggaaaatc cctgagaaac ttttgcaaag cctcgattct ttgcggcgcg
     2101 gcggcgacgg ctttcgcttt ttcaaattct tccgcatcgt tgactgattt tacagcgggc
     2161 ggttttttga cggtcgtcgg tctcgctttc ggtttctggg caaaagccgc gggcgataat
     2221 agaagaatga ttgataacag cagataaaaa cttttcatgc taaacctttg tttgtattgt
     2281 tcgcgtccat tttgcggttt tggataattt aaccacgaaa cgaggagaaa agacacgcgc
     2341 aatttaatct atacagttta tcatttaaca aactcgggcg ataaatgtta aatgttaaat
     2401 gatgaacgaa ctcaacgaat tctggacaaa ctcaatcaac gaagccatcg ataaagcccg
     2461 cgccgagggg cgcggcgacg tcgccgatta tctcacgctg aaggcgacga acgacgaaat
     2521 tcgcgccgct tcgtgccgct ggcttttcga gtccctgctt gaaatcgcgc acgagacgat
     2581 gcgcggcggg ttgaatctga aaatcgaaaa cgaaaacccg tatcgttttg aaatgaacaa
     2641 ggcgacgatg gtcggctcgc tgatggaatt caaattcggc attcgccgtc tgagcgccga
     2701 agccggctgg acgcggacgc cgcaggacgg ttttatgcgc ggcggcgcgc tcgcctttgc
     2761 ccgcctcgcg catttcgggc aggcgaaaca caacgccgag ctgattctga ttcgctccgc
     2821 cgattccgct ccgcagtggt tcgcggtcga ccgcgcgggc aggcaaacgc cttttgattc
     2881 aaacaactta cgcgagcatt tcaggatttt tctgggctga atttcagacg cggctaatta
     2941 tcaaggcgtt gctttcgcgt tgcaagcaaa taaataatat gtgaaaattt tctattgaac
     3001 tcgttttatc aggagatttt taatagtgaa gtcagaactg gaaaagttaa ttgagctgca
     3061 aaaaaccgat accaatatca gaaaactaaa aaaagccatc gaaacatcag acgaaaggcg
     3121 tgccgcgatt gaacgagagt ttgaacagca cgcctcttct attcgcgaaa tacagacgag
     3181 ccgcgacggc gctcgctccg agcgcgagcg gctggaagcg caaatcgccg aaaataaaac
     3241 ttatctcgaa cgcgccgacc gcaatctgaa acacgcgcaa aaccagaagg aatacgaaac
     3301 cgcgatgcgc gagaccgacg ccctgcaaaa acagattgcc gcgctcgaag cgcaggttat
     3361 ggaaaaaatg acgacgctcg aagaagtgga aaaagtttta ggcgagcgct cggaagaaat
     3421 ttccagcctc gaatccaatc gccagggcga gctggaaact tttgaaaccg agcttgccgc
     3481 caaccgcgcc gagctcgacg gcgaaatgaa aaaacgccac gaagttttcg tcacgctgcc
     3541 ggcaaatctg gcaagcgttt acaaccgtat ggcgcagcgc agccgcgacg gaatcgccgt
     3601 cgccgaagta aaaaacggct cgtgcagcgc gtgttttatg aagctgcgcc cgcagatgca
     3661 ggtcgaaatc aaaaaaggcg accggattat cacctgcgaa agctgcgcgc gaattttgta
     3721 tatcacgccc gaagcgaaaa cggcgacgca ggaaagttaa tttgaatttt cgaaaacgaa
     3781 tttacgaagc gggcgcacaa tcaaatgcgc ccgctttcgt tttcggctaa attgaaagta
     3841 taaaaaatct tatgaaattt aacctcagag ttacacggat aaacgcagat tttaacagga
     3901 tgaacagcat aggcaggata aatacgaaag ttcgacatct tagttttaaa ttatcctgat
     3961 tatcctgttc atcctgttaa attatccgcg tttatctgcg gaaatctgtg gtttcgcttt
     4021 cttacttcgc ccattcgtcc gcgatttttg aatcgacttt gacgggaact tttttgatgt
     4081 attcctcgcc cgccgcgcac atcgcgtctt ccagattttt ctgcgtcgtt tcggcttccg
     4141 gcgcgtcgca ttcgacgatt acctcgtcgt ggacgatatt caccagccgc gccgaggttc
     4201 cgttgaattt atcgtgcaga aggcgcaggg cgcgtttgag aatatcgctc gacgtgccct
     4261 gaatcggcat atttttgccg ttgcgctgcg cggcggcgat ttgggcgtgg tcgttttcgt
     4321 caaagcggaa gcgcatcatt ctgccggtcg ccgttcgggc gaggcgctcg gctgtaactt
     4381 ttcgcgccgc atctctgagc cacgcgtcga gcccgcgata agtagcgaaa tagcggcgca
     4441 ttatgttttc ggcttcggtt tgcggcaagc cggtcatcat agcgaaacgc gacgcgccga
     4501 tgccgtagac gacgccgaaa ttcaggcgtt tggcaaacga gcgctggtcg ggcgtgacgt
     4561 cttcgggttt gacattgaaa acctgcgcgc cggttatagt gtgaaaatca gcgccggatt
     4621 gaaaagcctt gataaaattt tcgtcgttcg agaaatcagc taagattcgc agctcgacct
     4681 gcgaatagtc ggcgatgacg agtttcttgc cttcgggcgc gcggaagcaa cggcgatatt
     4741 cttcggcgtg cgggatttgc tgcaagttcg ggttgttgca ggaaaatctg ccggtcggcg
     4801 cgccgatttg gcgaaaatcg gcgtggattc tgcccgtctg cggcttgata aattccagaa
     4861 tgttttcgcc gaagctggtc agagatttcg ccacgcttcg atattccagc aatttcgcca
     4921 cgaccggata atcggcggcg agcggctgca gttgccagcc gcgcgtcgtc gccggaaccg
     4981 gaacgcccag gtttttcagc gcgtccgtga cctgcgcctg cgaatcgaga ttgatttccg
     5041 ccgcgccgaa cagggaagcc tgcgcgacgc ccgccgaaag catctgctgc aattcgagcg
     5101 cgacgaccgc ctgttccttt tttacttttt cgagctgctc gcgccagcgc gcctcgtcca
     5161 gataaaatcc gttcaattcc attgccgcga ccggcatcac gcagtcgaat tcgagcttgg
     5221 cgacttttat caaatcgtcc gtctttagtc tttcgacgat tttttcccga agcggaacca
     5281 ttgtcgcggc gtcgcgggcg gcgtattcga tttgcgccgg cgataattcg ggcgcgttcc
     5341 aatcgctgac ctgttcgctt ttgtccagct cgacgccgag aaaatacgac gagacttccg
     5401 ccagattgtg acgccggtcc tggtcgcccg cggcgataag ctggctggcg agcagggtat
     5461 cgaaaattcc gttgatgtcg acgccgagat agtgttttat ccatttggcg tcgaatttcg
     5521 cgttgtgcgc gacctttatc ggcttggtcg cggacagaag cctgcgcagc ggctcaagct
     5581 cgggcgtcgt tttcacgtcg ccgcggtcgg caaacgcttt gaggtcgatg acgctcgtgt
     5641 ccttgccgtc gctcaattga acgaggcgca cgatgccctg atacgggtcg agctcggtcg
     5701 tttcggtgtc gaagccgaga taatcggcgg cggacaattt ttcgcacgct tcgcgcagcg
     5761 gctcggcttc ggtgatgact tgaaaataaa tttccatact ggttaatcgt aaaacgtaaa
     5821 tcctaaatag tgaagccgct tcaaggtttt tcagaaggga aaaagatttc cggcaaattc
     5881 gtcaaattat tcttgccaaa atggattttt aatcaatatc atatctctac cggcagtttt
     5941 gaaaagcaat tttttataaa atttttggag gtgaaagctg tgcctacggc aagttgtccc
     6001 gaatgtagcg aaaaagttta tgtcgacgcc gattccgagc agggcgacct cgtcagttgc
     6061 gacgaatgcg gttcggattt ggaagtggtc gggctcgacc cgatcgagct ggacctttac
     6121 gaagataaag gcgagaaaga cgattacgac gaggtggatt ttgacagcct cgattaccag
     6181 aacgagagat tttagtgaaa aatgaaaaac gaaaagtgcg aaatgcaaaa tatcaggatt
     6241 tagaattcag aattcaaatt gattcttctt cctgttattt tgcatttcgc gttttgcatt
     6301 ttgcatttat ttcaaccggt atcccgcgcg attttgcttg acctgatagc ctttggtgga
     6361 ttcgattctg atttcgcgaa attttccgtt tgtcttatct tcttccgacg ggtaaaaagc
     6421 cagaacgtat gaagccgtga tttcctcggc gagagctttg aaaagcggct cgaaatcggt
     6481 ctgcgtcgcg tagaagcttt tgccgcccgt tttttcaatc agaccgctgg cttcgagcgg
     6541 cgtcagcaga ggttttttcg ttccctgcag gcgcgaaaac gacggcagaa ttaccgagta
     6601 aacgctggtt tccgcattat tggcgcgctc gatgacggtt ttcggcgaaa ccgtgtcgcc
     6661 gaccggaaag ccgtcggtta tcaaaatcac ggcgcgtttc attaatttgt tattgcgcgc
     6721 gtcgggcgct tttttccgca gcaggcgaat ggcgtcgtcg gtcgcgtcat aggcgtgcgt
     6781 cgagagcccg tcctggtctt ttaaaatctt ctcgaaggtt ttttccagtt tttcgcgctt
     6841 gttggtaaat gattgcagcg tttttacttc cattccgaaa gtgatgaccg cgaaatacga
     6901 ttgataatcg gcgaggcgct cgataaattt ttgcatcgcg tcgcgcaggc gaatcagctc
     6961 gtcgcgcgtc atactgccgg agacgtcgag cgcaaaaacg attgaaagcg gtcggctttc
     7021 gtccgattta cggagcggtt caaaaaaatc gacttttctt tccacgccgt tttcaaaaac
     7081 cttgaaatct ccggcttcga gcccgcgcac gggcttgccg tccttgtccg tcacgctcac
     7141 ctcaaaggcg accacgtcgg tttcgacgcg gattacatcg tcctgcgccc gaacgggcgg
     7201 aagccagccg ccagccgcca gcagcaggag aattccgaca cgaaaaaaac gcgagagaga
     7261 aaaccgtccg aatctcaaat tttgaattct gaataacatt atccgcattt tataagtctt
     7321 tttccggtga agcaaaaggt tttgctttgt ttgagagatt aggcgggcgc ttctcgttgt
     7381 ccgcaacttt cgccggcggc tcggaatcat tcattttgaa gtgattatgt caatatgaac
     7441 ggtcagtttg aacgaaaatt atttatccgc aatttatcgc gcttttttaa ccaacaatta
     7501 tctaaaatat tttatacgcg ccgcttcggc acgaaatttg ttatatattc gggcggctat
     7561 gtgccgtaga ttgagaattt ttgagaagaa gtatttgaga agcccgcaga tttttccgca
     7621 acaatctatt cagtttctga aacaatgcat aaaaatttag atttggaagt aaaaatcgtg
     7681 aaacaatttg acatctcaga atacagtagt ccgacgaaaa ttctgggcgg cgtcctgctg
     7741 gcgctcagcg cggcggtgct cgtctacaca atctacggct gtttttcttt cacgtttctg
     7801 caatttgtca ttttaggcgt ttcgatgacg gtcgcggcgc tcgccaatca ataccagttt
     7861 aagataccga aaacgcgtct gaccatctcg ccgcgcaaag tttttatttt ctggggaacg
     7921 atttggctcg ggtcggcggg cggcgttttg ctcagcctga tttcgtcaat cgttcgttat
     7981 cgcacgacga aaaacaaaat caaatgggtt tacggaatct ttattcacac ggttgcgacc
     8041 tttgcggcgg ctgaggtttt ttatcttttt ctgcaccagt ttgcaggctt tgacggctgg
     8101 aatgttgccg ccaactcgat taatctcgga tggcttctgc tcgccgccgc gctgatgacc
     8161 ctgacgcatt atgtgctgac gacgctgctc agctcgctgc tgctggcatt tgacagcgaa
     8221 gacggtttta gaagcatctg gaaaaaaaga tatacgcgca cgatagccaa ttatctggtg
     8281 gcttttgccg cgatgctggt tttgcatctt tcttttcgtc atttcggcgt cgatttcgga
     8341 ctggtcgtgc tgccgacggc gattttcggg catctcgcgt ataaaattca ccttcagagg
     8401 ctggcgcaga aaaccagaga aatcacggag gcgagccgca ttcatctggc gacggtcgag
     8461 gcgctggcga cggcgattga cgcgcgcgac caggtcggca tcgggcacgt gcgccggacg
     8521 cagatttacg ccgtcggcat cggcgagatt ttaggcttgt cggaaaagga aataaacgcg
     8581 ctgcgcaccg gcgcgctctt gcacgacatc ggaaagctcg ccgtgcccga ccatattttg
     8641 aacaagccgg gacgcctgac gccagccgag atggaaaaaa tgaagattca cgcttcggtc
     8701 ggcgcgtcga ttctcgaaga cgtcaaattt gactgcccag tcgtcccgac cgtcaaatat
     8761 catcacgaag cgtgggacgg aagcggatat ccggaaggct tgaaaaagga aaatattccg
     8821 ctgacggcgc gaattctgtc catcgccgac gcttacgact cgctgcgcgg cgcgcgtcct
     8881 ttccgcggcg cgatttcgcg cgacgaagcg cgcaaatctc ttttgaacgc ggcgggagcg
     8941 aaattcgacc cgaaactggt cgatattttt ctcagaaacc tgcaacattt tgaagcggaa
     9001 atcgaatcgc aggggctttc ctatcatttc gacggcgcgg aagccagtat tttcggcatc
     9061 gaggcgggcg aggcaaacga aaactacgtc gaccaaatca agcgcgccaa tcgcgaggtc
     9121 tttacgcttt acgaaatggc gaaagtcttt agctcgtcgc tgaatctgcc ggacacgatt
     9181 tcgcgtttta cggcaaaaat cggcgagctc gtgccgttcg acacgtgcgt cgtttacctg
     9241 ctcgacgaag cgcaggacgc ggcgaccgcc gtctatgccg aaggaaaaaa cagcgcggcg
     9301 ctcaaaggca agcgcgtcaa aagcggcgag ggcgcgaccg gctacgcttt aaagaaacgc
     9361 cagcccgttt acaatatcaa tccggcgctc gatttttcgt tcgagatgca cgattttatt
     9421 ctcgaataca cggcgatggc gtccttgccg ctgatggcgg gcgaacgtct tatcggcgcg
     9481 gtttcgctgt attcctgcga gctggaaaac tacgaagacg agcatatgcg tcttttggag
     9541 acgatttcgc gcatcgcggc ggacgcgatt tccaaatcgc ttcatcttgc cgaaaccgaa
     9601 tcgcgcgcgc tgaccgaccc gatgaccggg ctgccgaacg cgcgcagcct gcaggcgcag
     9661 tttgaaaacg aaatcgcgcg cgcccgccgc aacggcagcg agtttcaggt tttaatgctc
     9721 gacctcgacg gctttaaatt ggtcaacgac actttcgggc acaaagtcgg cgacgtgctt
     9781 ttgcgcgaaa tttccaaagt gatgcgcgga cagcttcgcg attacgattt tctggcgcgt
     9841 tacgccggcg acgagttcgt cattatcgcg cccgaaatga ataacgaagg cgtaagcgag
     9901 ctttgccagc gaatggaaaa agccgtcgcc gattttgttt tgccgatcgg cgacggcaga
     9961 tttgcgcagg tcggcgtcag tctgggcgcg gcggcgtacc cgcgcgcggg cgacacgctc
    10021 gaccagatta tcgtcgccgc cgaccaggcg atgtatgccg tcaaagcggc gcgcaagcga
    10081 aagcagaatc aaccgccggt tcagaccgaa tcgggcgctc agccgctgct gccggaggcg
    10141 cacgaatttc cacatatgtt gattgccgaa gaagccttaa ttgtcgaact ggacgaaagc
    10201 cacatcgttt cggcggcaat caactaaaaa taagtaaaaa gataaaaggt aaaagtaaaa
    10261 gggcaaaaag cgaaagtaag aaacgcgttc ctgtttttgc cttttgcctt caaattctct
    10321 gtgatgaatt ctttctaaat tatgccacgg tgatttcctc gctcaggtag acgtcctgaa
    10381 tcgcgtttaa aagctcgacg ccttccgcca tcgggcgctg gaaggctttg cgaccggaaa
    10441 ttaagccggt gccgccgccg cgtttgttga cgaccgccgt gcgcacggcg tccgccaaat
    10501 cgcccgcgcc tttcgattcg ccgccggaat tgattaaccc gcagcgtccc atataacagt
    10561 tcgctacctg ataccgcacg aggtcaatcg gattgtcgct ggtcaaatcc gtgtagattt
    10621 tcggattcgt tttgccgtag ctgctttcca agccgagaac gttgtagccg ccgttgcgct
    10681 cgggcagttt ctgcttgatg atgtcggctt gaatcgtcac gccgagatga tttgcctgcc
    10741 cggtcaaatc ggcggcggtg tgcaaatcgc cttccttggt tttgaatttg ttgttgcgca
    10801 gatagcacca caaaatcgtc gccataccta attcgtgcgc gtaggcaaac gcttcggcga
    10861 cttcgccgag ctggcgcgta gattcgtccg agccgaaata aacggtcgcg ccgacggcga
    10921 ccgcgcccat atcgcggcac tggtcgatgg cgccgaacat cacctggtcg aatttgttcg
    10981 ggtaagtcaa aagctcgttg tgattgattt tgacgacgaa cggaatttta tgcgcgtatt
    11041 tgcgggcgac cgagccgagc acgccgaagg tcgaagcgac ggcgttgcag ccgccttcga
    11101 ttgccagttt cacgatgttt tccgggtcga aatactgcgg attcggcgcg aaagaagctc
    11161 cggcggaatg ctcgatgccc tgatcgaccg gcagaatcga gagataaccc gttccgcgca
    11221 gcctgccgtt gtcgaaaagc gtctgcaagg cgcgcagggt cgccggcgtc ctgtccgaca
    11281 gagtccagac gcggtcgaca aaatcgccgc ccggcagatt caaggtttct ttgggaatcg
    11341 tcttcgaagt atgatttaaa agcgtatcgg cgtccgcgcc taaaagctcc tgtattttac
    11401 tgtaatcaac agccatcaaa tgttctcctg ttaataaaaa gatttaattt tgatatgaaa
    11461 tttcaatggt aatcgagcaa ttatagcata aataattctt gcgtttttga aaaacgacgc
    11521 gcgcgcattt gaaaaattcc gggatattag gcatcataat acgtttgctt ctattttccg
    11581 gcgctgctcc cgctacggca gccggcttcc gaagagtttc cggtccgcgc tcgttaaacg
    11641 cggcgagatt ttactcataa agtcgagata attttatgct cagcttcgtt caaaggtccg
    11701 cgcttttgct tttattaatg tatcttgttt gtctgtctgc cgaatccgcc gaggcgcaga
    11761 ccgccgagtg gcgaccggtt tctccggcgg aactcgaaat gaaaacgccg caggtcgagc
    11821 cggacgccga cgccgaagcg attttctggg aagtgcggct ggacgacaaa aaaagcaata
    11881 agctctcata caatcactat gtgcgcgtca aaatcttcac cgagcgcggg cgcgaaaaat
    11941 tcagcaagtt cgacattcct ttttataagg gcagaaaagt cgaagacgta gcggcgcgcg
    12001 ttatcaagcc ggacggcacg acgattgacg tccgacccgc agacattttc gagcgcgaga
    12061 ttatcaaagc cggaaaaatc aagattcgcg caaaatcgtt tgccgtcgcc ggtatcgagc
    12121 cgggcgtgat tgtcgaatat cgctacgtgg aagtcgttaa aaacgattcg ctgaacaacg
    12181 agcgcctgat ttttcagcgc gacataccga tgcagagagc gacttattac gtccgcccgt
    12241 atcagggcac gattctcagc ttcgattttc gcaatatgca ggcggttcgc tttgtcgaaa
    12301 gctcggacgg cttttttgtc ggaacggcgg cgaatgtgcc ggcgctgaaa gaagaagcct
    12361 atatgccgcc ggaggacgaa gtccgccgct gggcgcgtct ttactataac ggctacaact
    12421 cgttttcctg gaacacgtta agctatcagg tcacacagac gttcaataaa ataatcaaac
    12481 cgaccaaaga gctcaatcag aaagcggctg agctgacggc gggcgcgacg acggatgaag
    12541 aaaagctccg gcgcatttac gactttacgc agaagcgcat aaaaaacgtc aatttcgatt
    12601 cttcatacac cgaagaacaa ttggaatcaa tcaaaatcaa ggatgccgac gacgttctga
    12661 aacgcggcgt cgccaattcg gtttttgtca actggctttt cggcgcgttg gcaaaagccg
    12721 ccggttttga agtaaacgtc gttctggcgg ctgaccgcag cgaacatttc tttaatccga
    12781 acgataatat aagcccgggc tatctgcatc cggccggcat cgccgtgcga atgaaagacc
    12841 aggaaaagcc tcagcagata aaacccgtaa ccggcggagc acacgcctcg tcggggcgtt
    12901 cgacggcagg gcagacgtgg aagtttttcg acccgggaac gctttacctg cccttcggca
    12961 gaatggtctg gaacgaagaa gacgtctacg cgctgctggt cggcgaaagc agttcccagt
    13021 ggagaaaaat tccgctctcc ggagtcgggc tgtcgcccgc ccgccgcgcc ggtaaattca
    13081 aactggcgga agacggaacg ctggaaggct ccgtcaggct cgaatatgaa ggtcatcagg
    13141 cgatttcgcg gcgccgcgcc gaatttaagg attcgcccgc caaacgcgag gagaatctta
    13201 aggaagaaat caaaaaacgc atcagcgcgg cggagatttc cgacgtgacg attgaaaatt
    13261 ttgataatcc ggctttgcct ctgacttata attttaagat tcgcgtgccg aattacgcgc
    13321 agaaaaccgg caagagactc ttttttcaac ccggattttt cgaatacggg acaactcccg
    13381 tattttcgtc ggcaacccga acgcatagtg tatacttcca atatccctgg tcggaacagg
    13441 atgatgtcga attcgagctg ccgaaagatt tttcgctcga taacgccgaa gcgcccgccg
    13501 atgtcgccga ctcacagcaa atcggcagcc tgaaaatcaa tatcggtatc gataaggtca
    13561 acggtaaaat catttacaag cgaagctttt atttcggcaa cagcgggctt ttattatttc
    13621 cggtttcgaa atacgagcct ttgaagagct tgtttgactc gtttcacaaa gccgataccc
    13681 acacgattac gctgaagcag aattaaaatt gttaacaagc cgatttttct tgcgtataag
    13741 aaaatttttg atatacaatg ccgcaattaa aggcaaataa aactattcag cccacagctc
    13801 tgaagctgta gaaatacggc gaatctatat aaaaatgaat ctattaaaga cgaggttagt
    13861 aatgatgttt tactctaatc ggaagcgttt cgctctgctt cccgtttttt gcctgct
//
LOCUS       DASWNE010000233         5736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_607382, whole genome shotgun sequence.
ACCESSION   DASWNE010000233 DASWNE010000000
VERSION     DASWNE010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5736
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607382"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1973
                     /locus_tag="VGB68_04760"
     CDS             <1..1973
                     /locus_tag="VGB68_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEY0048572.1"
                     /translation="MFKDAYMQEAIVDKVRAVEFDMESSANVEVGSLLDSVSDMSNGF
                     ERIADEDEAVSEQSPEETKSGKSKRGRGGRKSRKDQTSPETAVEAATTAAGESGNEEA
                     ATEDSQSGRGGKSKKENRKSKEQKTEGREKSSRGRKSSKNKDEGEALADGQASLTEPA
                     PDIAEMAVRRGGRRRGGGGGGGGNRGNRPPQQARSGPTEDRYEGSKMDETISSAETEF
                     AGAEMVENSGGNGGSGYQQGGRRESRRERERNQPTITDLLREGQEILVQIAKEPIARK
                     GARITSHIALPGRYLVYMPTIEHVGVSRKIESGGERNRLRTLIQKIRNEEDVPSGGFI
                     VRTAGIGVPEENLREDARYLVRTWKDVKKTAEKSKSPAVVHQDLDIIQRILRDQLSDD
                     FTAIRVDSEEEYLRIVEFVNRVQPKMVNRVKLYVRDEPILEAYNVQEEIDKALKPRVW
                     LRSGGYLVINQTEALVAIDVNTGKFVGKGNARLEDTITKTNMEAVEEISRQIRLRDLG
                     GIIVLDLIDMEDRRNRQKVLQALQEALSYDKSPTKVLSFNDFGLIIMTRKRVKQSLER
                     TLCAPCAHCQGAGWVKSPQTVCYNILEEARRLAKHADDVRLVTLRVHPDVVKALRTDD
                     KFVMEEIEAYLGPIDVTSDPTVHEEQFDFAFI"
     gene            2197..3135
                     /locus_tag="VGB68_04765"
     CDS             2197..3135
                     /locus_tag="VGB68_04765"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEY0048573.1"
                     /translation="MLRRKIVFFLLIFACLTPFVSPPIALALGLIPALGFGNPFPEQT
                     RKTTKILLQISVILLGFGMNLEAVVKAGKDGILFTIATIFGTLALGFLLGRFLKIEDK
                     TSALISSGTAICGGSAIAAVAPAIEADSDEISVSLGTVFILNSVGLFVFPVVGRALNL
                     TENQFGVWAAIAIHDTSSVVGAASRYGAEALQIATTVKLARALWIAPVALLFAFIYRK
                     KTAQKKKKIAIPWFIFFFLLATVARTYAPPFILPSLFDAPVMLAKTGLTVTLFLIGAS
                     LSRATLRKVGVKPLVQGVLLWIVISLVSLWAVIQIL"
     gene            3315..3572
                     /locus_tag="VGB68_04770"
     CDS             3315..3572
                     /locus_tag="VGB68_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048574.1"
                     /translation="MEKDKFEQSQKIPSVPQETKGVINPRLLRNVAFGVITFSLVICT
                     VLCILAIWEFTKSDAVWRAIATFVVVSVAIGILTFVNEKLG"
     gene            3624..4709
                     /locus_tag="VGB68_04775"
     CDS             3624..4709
                     /locus_tag="VGB68_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048575.1"
                     /translation="MNPAKCALLFVVCSMAFFGAAACGRSSRQAPTDPKTLNPGIDPQ
                     LIMFGGWRDADKGIFLAKSEIGTQERVYFALAKPNGEPVLDDSAPKIEINIGSNFPNI
                     AGANVGGKSAQFYTKTDDSGKITKAGIYIAYSSKSNKESQHFDLEPVTHTLHGWSSND
                     VYAVFSAEGKKVFMVDDNSSRVWDIKLQNITSFNQVALAGDKRGFYIVGDTTLPGFGL
                     EYVDFNKYTDYFNRKTQSFTILNVPKPKDAQATFRALISPDQKTLALLTNTHPVGAGG
                     DFHPDAENKLSLYDLQTRQLTREYELPRGAADLRNMLFVSGPWWRADNRALGFDLRPT
                     REQKTFYTIDLSTGEITNWFTSGNSPK"
     gene            4841..>5736
                     /locus_tag="VGB68_04780"
     CDS             4841..>5736
                     /locus_tag="VGB68_04780"
                     /inference="COORDINATES: protein motif:HMM:NF036484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048576.1"
                     /translation="MSINKITVRGARQHNLKNINVEIPRNKLTVVTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLDQLEKPDVDSVEGLSPAISIEQKTVSRSPRSTVGT
                     VTEIYDYLRLLFSSIGQPHCHLCGAPITRQSVEQITSGILNLPEGERVMILAPLVRGR
                     KGEFKKELEKLNKDGFLRARIDGEMRQLDEEIKLDKRKNHTIEVVVDRLLIKEGVRER
                     LTESVKTALKLTGGAVLISIIDGEEKLYSEKMACVNCGVNIPTLEPRSFSFNSNYGAC
                     RKCQGLGTVMQIDPSKIVPDANP"
BASE COUNT         1424 a         1477 c         1554 g         1281 t
ORIGIN      
        1 agatgttcaa ggacgcttat atgcaggaag cgattgtcga taaagtgcgc gccgtcgaat
       61 tcgatatgga aagcagcgcg aacgtggaag tcggctcgct gctcgactcg gtctccgata
      121 tgtcgaacgg tttcgagcgc atcgccgacg aagacgaagc cgtctccgag caatcgccgg
      181 aagaaaccaa gtccggcaaa tcaaaacgcg ggcgcggcgg cagaaaatcg agaaaagacc
      241 aaacctcgcc agagaccgcc gtcgaagcgg cgacaaccgc agccggagaa tccggaaatg
      301 aagaagccgc gaccgaagat tctcaaagtg gacgcggcgg aaaatcgaaa aaagaaaatc
      361 gaaaatcgaa agagcaaaag accgaaggca gagaaaaatc gagcagaggc agaaaatctt
      421 cgaagaataa agacgaaggt gaagcgctcg ccgacggtca ggcgagcctg accgagccgg
      481 cgccggatat tgcggaaatg gcggttcgtc gcggcggtcg tcgtcgaggc ggcggcggtg
      541 gcggcggcgg taatcgcggt aatcgtccgc cgcagcaggc gcgcagcgga ccgaccgaag
      601 accgttacga aggctcgaaa atggacgaaa ctatttcgtc agccgaaacc gaatttgccg
      661 gagcggaaat ggttgaaaac agcggcggca acggcggcag cggctatcag cagggcggca
      721 ggcgagaatc gcgccgcgag cgcgagcgca accagccgac aatcaccgac ctgctccgcg
      781 aaggtcagga aattctcgtc cagattgcca aagagccgat tgcccgcaaa ggcgcgcgca
      841 tcacttcgca catcgcgctg cccgggcgtt atctggttta tatgccgacg attgaacacg
      901 tcggcgtttc acgcaaaatc gaatcgggcg gcgagcgcaa tcggctgcgc actctgattc
      961 agaaaattcg caacgaggaa gacgttccgt cgggcggctt tatcgttcgc accgcgggaa
     1021 tcggcgtgcc ggaagaaaat ctgcgcgaag acgcgcggta tctcgtccgc acctggaagg
     1081 acgtaaagaa aacggcggag aaatcaaaat cgcccgccgt cgttcatcag gatttggata
     1141 ttatccaacg aattttgcgc gaccagcttt ccgacgattt cacggcgatt cgcgtcgatt
     1201 ccgaagagga atatctgcgg attgtcgagt tcgttaatcg cgtccagccg aaaatggtca
     1261 atcgcgtcaa gctttacgtg cgcgacgagc cgattctgga agcctacaac gtgcaggaag
     1321 aaatcgacaa ggctctgaag ccgcgcgttt ggctgcgttc cggcggctat ctcgtcatca
     1381 atcaaaccga agctctggtg gcgattgacg tcaacaccgg aaaatttgtc ggcaaaggca
     1441 acgcgcggct cgaagatacg attacgaaaa ccaatatgga agcggtcgag gaaatctcgc
     1501 ggcagattcg cctgcgcgac ctcggcggaa tcatcgtgct ggatttgatt gatatggaag
     1561 accggcgcaa tcgccagaag gttttgcagg ctttgcagga agcgctttcg tatgacaaat
     1621 cgccgactaa agttttgtcg tttaacgatt tcggcttgat tattatgacg cgcaaacgcg
     1681 tcaaacaatc gctcgagcgc acgctctgcg ccccgtgcgc gcattgtcag ggcgcgggct
     1741 gggtcaaatc gccgcaaacc gtctgctaca acattctgga agaagcgcgc cggttggcga
     1801 aacacgccga cgatgtgcgc ctcgtgactt taagagttca cccggatgtc gtcaaggctc
     1861 tgcgcaccga cgataagttc gtgatggaag aaatcgaagc ttatctcgga cctatcgacg
     1921 tgaccagcga cccgaccgtt cacgaggaac aattcgattt cgcgtttatt taaaatggaa
     1981 atttaacgct ttccgtaagg gcaggacttc gagcttgccc ttatttttgt ttaagcggaa
     2041 acgatgagaa tgcaaaaccc ggaaggcgaa atgaaagaaa tcggaagccg cgagccggta
     2101 gagtttatct tccgatttct cgcttttaac ttccgatttc ggtcttctat cttcttcatt
     2161 ttgcgctctg ccttttgaat tttgcgttag ctatttatgc tccggcgaaa aatcgtcttt
     2221 tttcttttaa tctttgcctg cctgacgccg tttgtttcgc cgccgatagc tctggcgtta
     2281 ggcttgattc cggcgctcgg cttcggcaat ccgtttcccg aacagaccag aaaaaccaca
     2341 aaaattcttt tgcagatttc cgtcattctg ctcggtttcg gaatgaatct ggaagcggtc
     2401 gtcaaagccg gaaaagacgg gattttgttt acgattgcga cgattttcgg aacgctcgcg
     2461 ctcggctttc tgctcggcag atttttaaaa atcgaagaca aaacgtcggc tctgatttcg
     2521 tcgggcacgg cgatttgcgg cggcagcgcc attgcggcgg tcgcgcccgc cattgaagcc
     2581 gattcggacg aaatttccgt atctttagga acggttttta tcttaaattc ggtggggctt
     2641 ttcgtttttc cagtcgtcgg tcgcgcttta aatttaaccg aaaatcaatt cggcgtctgg
     2701 gcggcgattg cgattcacga taccagttcc gtggtcggcg cggcgtccag atacggcgcg
     2761 gaagctttgc agattgccac gaccgtcaag cttgcgcggg ctttgtggat tgcgccggtc
     2821 gcgcttttat tcgcctttat ctaccgcaaa aaaaccgcgc agaagaaaaa gaaaatcgcg
     2881 attccgtggt ttatcttttt ctttctgctg gcgaccgtcg cgcgaactta cgcgccgcct
     2941 ttcattttgc cgagcctttt cgacgcgccg gtgatgctgg caaaaaccgg cttgaccgta
     3001 acgctttttc tcatcggcgc gtcgctttcg cgcgccacgc ttcgaaaagt cggcgtcaaa
     3061 ccgctcgtgc agggcgtttt gctctggatt gtaatttcgc tggtttcgct gtgggcggtc
     3121 atacaaattt tgtaaaatta ttgaacttaa acaaattatc gctcataata ggcatcgaaa
     3181 tatttaagat taaacgtaag aagcgagttt tgccggcaat ttttgaagct gaattgcgga
     3241 aaacccggag cggctgaaaa gcctctttga gataacgcgt cccggaaaat caaaagtagt
     3301 tttcacgaag caaaatggaa aaagacaaat tcgaacaatc gcagaaaatt ccgtccgttc
     3361 cgcaggaaac gaaaggcgtt atcaatccgc gtttattacg caacgtcgct ttcggcgtga
     3421 ttacgttttc gctcgtcatc tgcacggttt tgtgtattct ggcaatctgg gaatttacca
     3481 agtcagacgc cgtctggcgg gcgattgcga cgtttgtcgt cgtcagcgtc gccatcggaa
     3541 ttctcacttt cgtcaacgaa aaactcggct aaaatttttc aatcaagccg agccggtttc
     3601 tataatattt attcgataaa attatgaatc cagcaaaatg cgctctgttg tttgtcgtct
     3661 gttcgatggc gtttttcggc gcggcagcct gcggcaggtc gtcgcgacag gcgccgaccg
     3721 acccgaaaac cctgaatccg ggaatcgatc cgcagctcat aatgttcggc ggctggcgcg
     3781 atgccgacaa aggtatattt ttggcgaaaa gcgagattgg tacgcaggaa cgcgtatact
     3841 tcgcgctcgc caaaccgaac ggcgaaccgg tcctcgacga tagcgcgccg aaaatcgaga
     3901 taaatatcgg ctctaatttt cctaatattg ccggagcgaa tgtcggaggg aagagtgcgc
     3961 aattttacac aaaaaccgat gattccggca aaatcacaaa agccggaata tatatagcgt
     4021 attcgagcaa atcaaataaa gagtcgcagc atttcgacct cgaaccggtg acgcacacac
     4081 tgcacggctg gtcgtcgaac gacgtttatg ccgttttctc cgccgaagga aaaaaggtgt
     4141 ttatggtcga cgacaactcc agccgggttt gggacatcaa gctgcaaaat atcacttctt
     4201 ttaatcaggt cgcgctcgcc ggcgacaaaa ggggttttta catcgtcggc gacacgacgc
     4261 tgcccggctt cgggctcgaa tacgtcgatt tcaacaaata caccgattac ttcaatcgaa
     4321 aaactcaaag cttcaccatc ctgaacgtgc cgaaaccgaa agacgcgcag gcgacgttcc
     4381 gggcgcttat atcgcccgac cagaaaactc tggcgctgct gacaaatact cacccggtcg
     4441 gcgcgggcgg cgattttcat cccgacgccg aaaacaaatt gtcgctgtac gatttgcaga
     4501 ccagacaatt aacgagagaa tacgaactgc cgcgcggcgc ggcggattta aggaatatgc
     4561 tttttgtgtc gggtccgtgg tggcgcgccg ataaccgggc tctcggcttc gacctgcgcc
     4621 cgacgcgcga gcagaaaacc ttctacacga ttgacctctc gaccggcgaa attacaaact
     4681 ggtttacctc cggcaattcg ccaaaataat tgtctcttcg aatcaatcga ataacgcgcc
     4741 cgaacgctct ttgcttgtaa tgccgaagat ttgctaaaat aaaggtttct gctgtaggcg
     4801 atattttcat ccgtgaaagg cagaaacttt tatttaaccg atgagcatta ataaaattac
     4861 ggttcgcggc gcgcgccagc acaatctgaa aaacatcaac gtcgagattc cgcgcaacaa
     4921 attgaccgtt gtgacgggct tgagcggctc gggaaaatct tctctcgcct tcgatacgat
     4981 ttacgccgaa ggtcagcgca ggtatgtcga atcgctgtcg gcttacgcgc gccagttttt
     5041 agaccagctc gaaaaaccgg acgtcgattc ggtcgagggt ctttcgcccg ccatctcgat
     5101 cgagcagaaa accgtttcgc gctctccgcg ctcgaccgtc ggcacggtga cggaaatcta
     5161 cgattatctg cgccttttgt tttcgtcaat cgggcagccg cattgtcatc tgtgcggcgc
     5221 gccgattacg cgccagtcgg tcgagcaaat cacgagcgga attttgaatc tgccggaagg
     5281 cgagcgcgtg atgattctcg cgcccctggt gcgcgggcgc aagggcgagt ttaaaaagga
     5341 actcgaaaag ctcaataaag acggttttct gcgcgcccgc atcgacggcg agatgcgcca
     5401 gctcgacgag gaaatcaagc tcgacaaacg caaaaatcac acgattgaag tcgtcgtcga
     5461 tagattgttg ataaaggaag gcgtgcgcga gcgcctgacc gaatcggtca aaacggctct
     5521 gaagctgacg ggcggcgcgg ttttgatttc gattatcgac ggcgaggaaa agctctattc
     5581 ggagaaaatg gcgtgcgtca attgcggcgt caatattccg actttagagc cgcgctcgtt
     5641 ttcgtttaac tccaattacg gcgcgtgccg caaatgtcag ggcttgggca cggtgatgca
     5701 gattgacccc agcaaaatcg tgcccgacgc caatcc
//
LOCUS       DASWNE010000234         1597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_42433, whole genome shotgun sequence.
ACCESSION   DASWNE010000234 DASWNE010000000
VERSION     DASWNE010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42433"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..714
                     /gene="folK"
                     /locus_tag="VGB68_04785"
     CDS             199..714
                     /gene="folK"
                     /locus_tag="VGB68_04785"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEY0048577.1"
                     /translation="MKNESVTAYISLGSNLGDRAGNLLLGVRAMMEANFEVCRLSAIY
                     ETEPVGVENHGLFLNMVAETCVTGVTPEQMMARMIRIEYLLGRMQKNEKAPRTVDLDL
                     LFYGKLQYQSPFLTLPHPRLHQRRFVLVPLAELVPHLVHPVVNRTIQELLEHTDDTSM
                     VRRWKPNLSRE"
     gene            786..>1597
                     /gene="panB"
                     /locus_tag="VGB68_04790"
     CDS             786..>1597
                     /gene="panB"
                     /locus_tag="VGB68_04790"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF001452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEY0048578.1"
                     /translation="MAYLQPDKPEKVYLPAIRAAKTKGEKLVCLTAYDYPTARIVDEA
                     GVDMILVGDSIGNVIHGYGNTIPVSLEEIVSATIAVKRATERAMVIADMPYGSYHVNE
                     DESVRNALRLMKYGGAEAVKLEGGRNRVDLVKRLVDEEIPVVAHIGLTPQSVYKLGGY
                     RVQGKTPDAAKRLIEDAKALEDAGAFAIVLELIPREIAEIMTRELKISTIGIGAGREC
                     DIQVLVLHDLVGFTFGRQPRFVRQYANIREVMNSAITEWMSDVKNGTYPSEK"
BASE COUNT          386 a          419 c          430 g          362 t
ORIGIN      
        1 ttacgaatta atgagttttg aactttgact tttgtcattt gccgatcgag cggcagcgaa
       61 attcaaaaat taaagttcaa agctcttttc tttcgattct tgatttttaa ttcttaattc
      121 ttaattcgta attgaccttt cgcccgtttt ctcgcaaact tgtttcgttt tatttattcc
      181 aacgccaaaa attaaaccgt gaagaacgaa tccgtaacag cctacatttc gctcggctca
      241 aatctcggcg accgcgccgg aaatctcctg ctcggcgtgc gcgcgatgat ggaagcgaat
      301 ttcgaggtct gccgcctgtc ggcgatttac gagacggagc cggtcggcgt cgaaaatcac
      361 gggttgtttt taaatatggt ggcggagacc tgcgtcaccg gcgtcacgcc cgaacagatg
      421 atggcgcgaa tgatacgtat cgaatatctg ctgggcagaa tgcagaaaaa cgaaaaggcg
      481 ccgcgcacgg tcgatttgga tttgcttttt tacggcaagc tgcaatatca atcgccgttt
      541 ctgacgcttc cgcacccgcg actgcaccag cgcagattcg tgctcgttcc gctcgccgag
      601 ctcgttccgc atctggttca tccggtcgtc aaccggacga ttcaggagct tttggagcac
      661 acggacgata cttcgatggt caggcgctgg aaaccgaatt tgagccggga gtaaagagtc
      721 aagagtcggg aataagaatc tgctctcgac tctcgactcc cgactcgcta gactgttaag
      781 aatttatggc atatttgcaa cccgacaaac ccgaaaaagt ttatctgccg gcgatacgcg
      841 ccgccaaaac aaaaggcgaa aaactggttt gcctgaccgc atacgattac ccgacggcgc
      901 gcatcgtcga cgaagcgggc gtcgatatga ttctggtcgg cgacagtatc ggcaacgtca
      961 ttcacggtta cggaaacacg attccggtaa gcctggaaga aatcgtttcg gcgaccatcg
     1021 ccgtcaaacg cgcgaccgaa cgcgctatgg tcatcgccga tatgccttac ggcagctatc
     1081 acgtcaacga ggacgaaagc gtccgcaacg cgcttcgatt gatgaaatac ggcggcgcgg
     1141 aagccgtcaa gctggaaggc gggcgcaacc gcgtcgattt ggtcaaacgt ctggttgacg
     1201 aggaaattcc ggtcgtcgcg cacatcggct tgacgccgca atcggtttac aagctcggcg
     1261 gctaccgggt tcagggaaaa actccggacg cggcgaaacg cttgattgaa gacgccaaag
     1321 cgctggaaga tgccggcgcg tttgcgattg tgctggaact gattccgcgc gaaatcgccg
     1381 agattatgac gcgcgagctg aaaatctcga ccatcggcat cggcgcgggt cgcgaatgcg
     1441 acatacaggt tctcgtcctg cacgaccttg tcggatttac cttcggtcgc cagccgcgct
     1501 ttgtgcgcca atacgcgaac atccgcgaag tgatgaattc cgcgattacc gaatggatgt
     1561 cggacgttaa aaacggcacg tatccgagcg agaaaga
//
LOCUS       DASWNE010000235         3236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_78428, whole genome shotgun sequence.
ACCESSION   DASWNE010000235 DASWNE010000000
VERSION     DASWNE010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78428"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..>3236
                     /locus_tag="VGB68_04795"
     CDS             190..>3236
                     /locus_tag="VGB68_04795"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0048579.1"
                     /translation="MNEAPKEQEALRKYLLGDLADKEEMRRIEEKSMLDDDFDEQLSV
                     AEDELVDEYLDGSLSAPERERFLKFFLISTERKEKLRFIRDLRKYAGNSPNVVLDAEQ
                     LSQEKKAFFNWRALFAMPALRFAALALIVFGLGFAVWRVAVYQTNQSGVEKANQSDVD
                     KGLAQLRTAFRGQRPTESRTTANFDYAPLSDTRSANQTAVTDERALRRAEGYLRDAVE
                     NESDAEAYHAQGLLYLAEKKFDEALDEFNRALERAPRNARLHNDTGAAYLEKVLLARA
                     DEKQSEVSRNINSSLQHFNRALELDENLLEALFNKALVLQKIPAPAQAREMWAKYLEK
                     DSDSQWADEARRRLQELESKKSHNLSAGELEQAFLKAFREKDEAGASRLISQNRELIT
                     GKYLPQKLVASFVEASGAERDEYLRALIYAGELEEKNIGDSSAKTLASFYAQVSDADL
                     ESLKQAQALMKNSYKLCLAGRLKESLKESERARELFTGAGDVYQAALSRFVIVYCLIK
                     IDGRIADSIPIAQDLTDFCRRSNYKWLLSHTLYWLAAAQRVTGDRAKAKDSYKNCLAL
                     AQETRDPQILQKILISFANQNKFVGQNEASLNYLQQAFDALADAQGLSLREKWRSYSD
                     GIEILSSAGFYRLAEAISLENFQLAKDLGDSSFIAFSQLDNGIVNAQAEDFAEARTWL
                     NEVKQNAETNPENDERTELLAKSLLTLGYLERKLKNYSQAEKFYDEALSIVESSETNF
                     FLYEIEKARLLTNISLNKNAEVEAQIPRIINLAETYRKQIPDEHTRRSFFSSQQDIYD
                     IAVANSFNHARYEQAYGYLENSNSRSLLDWLKKGVSIKTEQKKIEVTFKENAQPLGLD
                     EIRAQMPDRVQILQYAVLENKVLVWLISKDSFTVEASEIEAEKLNEKISEFTGSVSAQ
                     SDKKQEKAKQLASELYELLISPVASRLDPEREICVIPHKILFPLPFAALTSPEGKPFL
                     AQFNFFYAPSANVFLLFTENARRKSAAANESLLSVGNPHFNRN"
BASE COUNT          919 a          789 c          806 g          722 t
ORIGIN      
        1 cgccaaaatc gaaacgcgcc gcaagctggc ggaaaaggac ggaatcagcg tcaatacgct
       61 gcaggtcaaa atgtttcgaa tccgcgcccg tttaaaggaa tgtctgcaaa aatgcatcgc
      121 cgaaaaaaac tcggcgtgaa aggtttttca gattttcgac ataacattat ggaacaagcg
      181 gaaaagaaaa tgaacgaagc cccaaaagaa caggaagctc tcaggaaata tctgctgggc
      241 gatctggctg ataaagaaga aatgcgccga atagaggaaa aatcaatgct cgacgacgat
      301 tttgacgagc aattatccgt cgccgaagac gaattggtcg acgaatacct cgacggctct
      361 ctttccgcac cggagcgcga acgctttctt aaattttttc tcatttcaac ggaaagaaag
      421 gaaaagctgc ggtttatccg cgatttgcga aaatacgccg gtaattcccc gaacgtcgtt
      481 ctggacgccg aacaactttc ccaggaaaaa aaagcctttt ttaactggcg cgccttattc
      541 gccatgcccg ctctgcgttt cgccgctctc gcgctgattg ttttcggtct cggcttcgcc
      601 gtctggcgcg tcgccgttta tcaaactaat caatccggtg tcgagaaagc caatcaatcg
      661 gatgtcgata aagggctggc tcaattgcgc accgcttttc gcgggcagcg accgaccgaa
      721 tcgcggacga ccgcgaattt cgattacgcg cctctttccg atacacgcag cgcaaatcag
      781 actgccgtca ccgatgagcg cgcgcttcgc cgcgccgagg gctatctgcg cgacgcagtc
      841 gaaaatgagt cggacgccga ggcttatcac gcacagggac ttttatatct ggctgaaaaa
      901 aaattcgacg aggctttaga tgaatttaac cgcgcgctgg aacgcgcgcc ccgaaacgcc
      961 cggctgcata atgacaccgg cgcagcttat ctggaaaaag tcctgctcgc cagagcggac
     1021 gaaaagcagt ccgaagtttc acgcaatatt aactccagcc ttcagcattt taatcgggcg
     1081 ctggagctgg acgaaaatct gcttgaggcg ctttttaaca aagctctcgt tttgcaaaaa
     1141 ataccggctc cggctcaggc gagagagatg tgggcaaaat atctggaaaa agattctgat
     1201 tcgcaatggg cggacgaagc gcgaaggcgg cttcaggagc tggaatcgaa aaaatcgcac
     1261 aatctttccg ccggcgaact tgaacaagct tttttaaaag cctttcgcga aaaagatgaa
     1321 gccggagcct cacggctgat aagtcaaaac cgggaattaa taacgggaaa atatctgccg
     1381 caaaagctgg tcgcgtcttt tgtcgaagcc tcaggcgctg aaagggacga atatttgcgg
     1441 gctttaatct atgccggaga actggaagaa aaaaatatcg gcgattcgtc agccaaaact
     1501 ctggcatcgt tttacgcgca ggtttccgac gccgatttgg aatcgctgaa acaagctcag
     1561 gctttgatga aaaacagcta taaactgtgt ctggcgggca ggctgaaaga atctttgaaa
     1621 gaatcggagc gcgcccgcga acttttcacc ggagccggcg atgtttacca agccgcactc
     1681 agccggttcg ttattgtcta ttgtctgatt aagattgacg gcaggattgc ggacagtatt
     1741 ccgatagcac aggatttgac ggatttctgc cgccggagca attataaatg gctgctttcg
     1801 catactcttt actggctcgc cgcagcgcag agagtgaccg gagacagggc aaaagcaaaa
     1861 gatagttata agaattgtct ggcgctggcg caagagacgc gcgacccgca gattcttcaa
     1921 aaaatcctca tttcatttgc caatcaaaat aaattcgtcg gtcaaaatga agcttcgctc
     1981 aattacctgc aacaggcatt cgacgcgctc gccgacgcgc agggcttgag cctgcgcgag
     2041 aaatggagaa gttacagcga cggcatcgaa attttatcgt ccgccggatt ttacaggctg
     2101 gcggaagcca tttcactgga aaactttcaa ttagcgaaag atttgggaga ttcctcgttt
     2161 attgcctttt cgcagcttga taacggcatt gtcaacgcac aggctgaaga ttttgcggaa
     2221 gctcgaacct ggctcaacga agtaaaacaa aatgcggaaa caaatcctga aaacgacgag
     2281 cgaaccgagc ttttagctaa atcgctcctg acattgggct atctggagag aaaacttaag
     2341 aattactcgc aggcggaaaa gttttacgac gaagcgttga gcattgtgga aagctccgaa
     2401 accaattttt ttctttacga aatcgaaaaa gcacgtctgc tgacgaatat ttctctgaac
     2461 aagaatgcgg aagtggaagc gcaaatcccc agaattatca atttggcgga aacttatcgc
     2521 aagcagattc ccgacgagca tacgcgccga agcttcttca gcagccagca ggacatttac
     2581 gacatagccg tcgccaattc atttaatcac gccagatacg agcaggcata cggttatctg
     2641 gaaaattcga attcgcgctc cttgctcgat tggttgaaaa agggcgtcag tattaaaacc
     2701 gagcaaaaga aaattgaagt tactttcaaa gaaaacgcgc agccgctcgg gctcgacgaa
     2761 attcgcgctc aaatgcccga ccgcgtgcag attctgcaat acgccgtttt agaaaataaa
     2821 gttctcgtct ggctgatttc aaaagacagc ttcacggtcg aagcctcgga aatcgaagcg
     2881 gaaaaattaa acgaaaaaat ctcggaattc accggttcgg tttccgcaca gagcgataaa
     2941 aagcaggaaa aagctaaaca actggcgagc gagctttacg agttgttgat tagcccggtt
     3001 gccagccgcc tcgacccgga gcgtgaaatt tgtgtgatac cgcataaaat tctttttcct
     3061 ttacccttcg ccgcgctgac ctcgccggag ggaaaaccgt ttctggcgca atttaatttt
     3121 ttctacgcgc cgagcgccaa tgtttttctg cttttcacgg aaaatgcgcg gcgaaaatcg
     3181 gctgcggcaa acgaatcttt attgagcgtc ggcaatccgc atttcaaccg gaacga
//
LOCUS       DASWNE010000236         6523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_642790, whole genome shotgun sequence.
ACCESSION   DASWNE010000236 DASWNE010000000
VERSION     DASWNE010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6523
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642790"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..439)
                     /locus_tag="VGB68_04800"
     CDS             complement(86..439)
                     /locus_tag="VGB68_04800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEY0048580.1"
                     /translation="MKQNNVIQEKTFAFAVRAVKVYKYLSEDKKEFIISKQFLRSATS
                     TGANIEEAIGGQSKADFIARLSISYKEAGETKYWIRLLQETDFFTDKEANSLLNDIEE
                     ILRIIAKIKISMSKT"
     gene            complement(477..2369)
                     /gene="tssF"
                     /locus_tag="VGB68_04805"
     CDS             complement(477..2369)
                     /gene="tssF"
                     /locus_tag="VGB68_04805"
                     /inference="COORDINATES: protein motif:HMM:NF017733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HEY0048581.1"
                     /translation="MRDELLGHYERELIFLRRMGAEFARKYPKIAARLLLDEEKAEDP
                     HVERMIEAFAFLTGRINLKLEDELPEITESFLNVLYPHYLAPIPSMAIAQFVYGTPND
                     KLTAVQKINRGAKLYSRPVDGTPCRFQTAYDVQLLPLEIISAALESPAPPDTRGRMAD
                     AYIRLSMRCYGDANLSEMKIGETGEPPEFLRFYLNDTPQLVFPLYELIFNHASAVEFR
                     AKEAPLGNKTMMTLTNVQLQIPDPVILPANEAVRQVGFGEAEGILPHTKRSFAGYRLL
                     TEYFAFPQKFLFFDIYGIDKAVGKKFGSHFDILIHLKDIQPPPGSITAETFSLGATPI
                     INLFQQTTDPVYLSQQKYEYHIIADVHHQATTEIYSIDEVFATDPRTNTTRTFSPFYS
                     VKHTYGERMEKAFWYANRRQSQRDDDNGTEMFVSFVDMSFNPRVPAEEVITVKATCTN
                     RDLPARLPFGGKENDFEVEGAALLSRVRCLTKPTETLRPPRRRAAQWRLISHLNLNYL
                     SMLETDAGVPEALQEILQLYNYNDSSVTRKQILGITGIESRRVVRQIGERIGAGFVRG
                     METTLEFDETEFVGSGMYLFACVLEKFFGLYVSLNSFNQMVLKTQQREGVIKRFPPRT
                     GEQVLL"
     gene            complement(2421..2906)
                     /gene="tssE"
                     /locus_tag="VGB68_04810"
     CDS             complement(2421..2906)
                     /gene="tssE"
                     /locus_tag="VGB68_04810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HEY0048582.1"
                     /translation="MARTDSEIRVTPSVLDRLLDFEPKSPQEAPKSRSRNLRELKMSV
                     RRDLEWLLNTRCFSEDIDENLEEVFKSVIVYGLPDITGISAKNHLEQKRLTRVMETAI
                     KTFESRLIDLKVTLEPVSSSDKALKFRIEARLDVDPTPEPIAFDTILQLGSGDFEVSE
                     K"
     gene            complement(3029..3520)
                     /locus_tag="VGB68_04815"
     CDS             complement(3029..3520)
                     /locus_tag="VGB68_04815"
                     /inference="COORDINATES: protein motif:HMM:NF017454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HEY0048583.1"
                     /translation="MANSDFYLKIDGIEGESEAVGFEKQIQVESWSMGANNAGSAGLG
                     TGLGTGKVSMQDFHFVMQNGKASAQIMLAVCKGNHIKEAVLSCRKTGGDGSPFTYYKY
                     TFNDLVLSSFQSGASAALPMEQVSFNYTKITLEYFQQKADGTVALTNTIAYDTKKVEG
                     TGA"
     gene            complement(3681..5171)
                     /gene="tssC"
                     /locus_tag="VGB68_04820"
     CDS             complement(3681..5171)
                     /gene="tssC"
                     /locus_tag="VGB68_04820"
                     /inference="COORDINATES: protein motif:HMM:TIGR03355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HEY0048584.1"
                     /translation="MAENAKQETAAVGTVDAPQETNLLDQILTEGRMARDEFQKEAAK
                     DMIAEFVNQVMSGELTMSKNMDVAINARIAEIDRLISAQMNEIMHHEEFQKLEGSWRG
                     LHYLLKNSLTSTQLKIRVMSVTKKELLKDFERALEFDQSSLFKKIYEDEYGTFGGAPF
                     GALIGDYEFGNHPQDMALLEKLSQVAATAHAPFLSAASPEMFGWDTFSEMSDVRDVSK
                     IFDRTEYMKWRSFRESEDSRYVGLTLPHVLMREPYGAATKPTESFRFEEDVDGKDHKK
                     YLWGNAAYALGTRLTEAFSMHGWCVAIRGVEGGGLVQDLPTHTFETDEGEIAMKCPTE
                     VAVTDRREKEFSDNGFIPLVHCKNTDYAAFFATQSANKSKKYDTDAANANARLSSQLQ
                     YIFAVSRFAHYLKSMMRDKIGSFMSRKECEIFLNRWISKYVLENDVAPTEQKAKYPLR
                     EARVDVAEIPGKPGCYRAVAFLRPHFQLDELSVSLRLVAELPPPAK"
     gene            complement(5176..5688)
                     /gene="tssB"
                     /locus_tag="VGB68_04825"
     CDS             complement(5176..5688)
                     /gene="tssB"
                     /locus_tag="VGB68_04825"
                     /inference="COORDINATES: protein motif:HMM:TIGR03358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HEY0048585.1"
                     /translation="MPSKESLQHKLERVRPPRVNITYDVEIGGAIELKELPFVVGVMG
                     DFVGKPEEPLPSLKQRKFVELDPDNFNQVMAGMKPRLAYSVDNKLQNDGSKLGVELKF
                     NGIEDFEPDSIVQQVEPLRKLVEARQKLADLRSKMDGNEKLESILEDVIGNADKQKQL
                     SDALGGAKEE"
     gene            complement(5750..>6523)
                     /locus_tag="VGB68_04830"
     CDS             complement(5750..>6523)
                     /locus_tag="VGB68_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048586.1"
                     /translation="EIDEGDMEGRANAIEWMEAQTAITVKTLPITGGEGFTYLNWEES
                     RSFDIPENIDALDYHEQEKAKLLKAQAAEENRKTGDMWRMAKAQTNRAFCEELNFTLE
                     ECWTECSALDRTIEEKFDRNQMPGLRNLKKSLEDIRTVLKPLLEEKRQQEPDAADEET
                     VETVEGENGTIQVRTVGAATGAIQSRQDALKRLADVSEYFKKNEPHSPVSYLVQRAVK
                     WGNMPLENWLQDVIKDETVIFQIRQTLGFNTKDPDSTES"
BASE COUNT         1415 a         1739 c         1622 g         1747 t
ORIGIN      
        1 attcaaaccg ttccggctcg tcgaaaagtt tttgatttaa aggcttccgc atcacaatta
       61 aaaattaaaa attaagaatt aaaaattaag ttttgctcat agatattttg atttttgcaa
      121 ttatgcgtaa aatttcttct atatcgttga gcaaactatt tgcttctttg tccgtaaaaa
      181 aatccgtttc ctgaagcagt ctaatccagt atttggtttc tccggcttct ttataggaaa
      241 tacttagtct ggcaatgaaa tctgcttttg attgtccccc gattgcttct tctatatttg
      301 cgccggttga agtcgcactt ctcaaaaatt gttttgaaat aataaattct ttcttatcct
      361 cggaaagata tttataaact tttactgctc tgacggcgaa cgcgaatgtt ttttcttgaa
      421 ttacattgtt ttgtttcatc tccaaaaaat cttaattctt aattcttaat ttttaattaa
      481 agtaaaacct gctcgcccgt ccgcggcgga aagcgtttta tcacgccttc gcgctgctgc
      541 gtctttaaaa ccatctgatt aaaggaatta agcgaaacgt aaagcccgaa aaatttttcc
      601 agaacgcagg cgaaaagata cattccgctg ccgacaaact cggtttcgtc aaattccagc
      661 gtcgtttcca tcccgcgcac aaagcccgcg ccgatacgct cgccgatttg gcggacgacg
      721 cggcggcttt cgatgccggt aattcccaga atctgcttcc gggtgacgga agaatcgttg
      781 taattgtaaa gctgcaaaat ttcctgcagc gcttccggca cgccggcgtc cgtttcgagc
      841 atcgaaagat aattcagatt cagatgcgaa atcagacgcc actgcgcggc gcggcggcgc
      901 ggcgggcgca gagtctcggt gggtttcgtc aggcatcgaa cgcgcgacag cagcgccgcg
      961 ccttcgacct cgaaatcgtt ttcctttccg ccgaacggca gacgcgccgg taaatcgcgg
     1021 ttcgtgcagg tggctttgac cgtaatgact tcttcagccg gcacgcgcgg gttaaagctc
     1081 atatcgacaa acgagacgaa catttccgtt ccgttgtcgt catcgcgctg cgactggcgg
     1141 cgattagcat accagaaagc tttttccatc cgttcgccgt aagtgtgctt gaccgaataa
     1201 aacggcgaaa acgtgcgcgt cgtattcgtg cgcgggtcgg tcgcgaaaac ttcgtcaatc
     1261 gaataaattt cggtcgtcgc ctgatgatgc acgtcggcta tgatgtgata ctcgtatttt
     1321 tgctgagaca ggtaaaccgg gtcggtcgtc tgctgaaaaa gattgatgat gggcgtcgcg
     1381 cccagactga aagtttccgc cgtgatgctt ccgggcggag gctgaatgtc tttcaaatga
     1441 atcaaaatat cgaaatgact gccgaatttt ttcccgacgg ctttgtcgat accgtaaatg
     1501 tcgaagaata aaaacttttg cggaaacgcg aaatactcgg tcagcaggcg ataaccggca
     1561 aacgaacgct tcgtgtgcgg cagtattcct tcggcttcgc cgaagccgac ctgcctgacg
     1621 gcttcattgg cgggcaaaat taccgggtcg ggaatctgta gctgaacgtt ggtcagcgtc
     1681 atcatcgttt tattgccgag cggcgcttct ttggcgcgaa actcgaccgc cgaggcgtga
     1741 ttgaaaatca gctcgtaaag cggaaaaacc agctgcggcg tgtcgttcag ataaaagcgc
     1801 agaaactccg gcggctcgcc cgtctcgccg attttcattt ccgacaggtt ggcgtcgccg
     1861 tagcagcgca tgctcaggcg aatataagcg tcagccatcc gcccgcgcgt gtcgggcggc
     1921 gcgggcgatt ccagcgcggc gctgattatt tccagcggca gaagctgaac gtcgtaagcc
     1981 gtctgaaaac ggcacggcgt tccgtccacc gggcgcgaat aaagctttgc gccgcgattg
     2041 attttctgca ccgccgtcag tttatcgttc ggcgtcccgt agacgaattg cgcgattgcc
     2101 atcgacggaa tcggcgctaa gtagtgcgga taaaggacgt taagaaagga ttcggtgatt
     2161 tccggaagct cgtcttcgag cttgagattg atgcgcccgg tcagaaaagc gaatgcttca
     2221 atcattcgct cgacgtgcgg gtcttcggct ttttcctcgt cgagcagcag gcgcgccgcg
     2281 attttcgggt atttgcgcgc aaattccgcg cccattcgcc gcagaaaaat cagctcgcgt
     2341 tcgtaatgtc ctaaaagttc gtcgcgcatc gcttaaatat caatatccca aagatttttt
     2401 atccgcttct gatgccttca tcatttctcg ctgacctcga aatcgccgct gccgagctgc
     2461 agaatcgtgt cgaaggcaat cggttcgggc gtcgggtcga cgtcgagcct tgcttcgatg
     2521 cgaaatttca gcgctttgtc ggagctgctg accggctcca gcgtgacctt taaatcaatc
     2581 aatcgagatt cgaaagtctt gattgccgtt tccatcacgc gcgtcaggcg cttctgctcc
     2641 agatggtttt tggcgctgat gccggtaata tccggcagcc cgtagacgat gaccgatttg
     2701 aaaacctctt ccagattttc gtcgatgtct tcggaaaaac agcgcgtgtt caaaagccat
     2761 tccaaatcgc ggcgcaccga cattttcaat tcgcgcaagt tgcgcgaacg cgatttcggc
     2821 gcttcctgcg gcgatttcgg ttcgaaatcc agcaggcggt cgagcaccga cggcgtcacg
     2881 cgaatttccg aatcagttcg tgccattcca agaaaaaaaa cacaccgttt gcccggcaga
     2941 cggcgttctg attaaaaaat atattttgaa tggtcgtctg ccgcttatgg cagcggcaga
     3001 caactttttt aactaatcgg gaagaagctt aggctccggt tccttcgact ttcttcgtat
     3061 cgtaagcgat ggtgttggtc aaagcaaccg taccgtcggc tttctgctgg aagtactcaa
     3121 gcgtgatttt cgtgtagttg aacgaaacct gctccatcgg cagcgccgcg ctcgcgcccg
     3181 attggaacga cgagagaaca aggtcgttaa acgtatattt gtaataggta aacgggctgc
     3241 cgtcgccgcc ggttttgcgg cagcttaaaa ccgcttcttt aatgtgattg cctttacaaa
     3301 ccgccagcat aatctgcgcg ctggctttac cgttctgcat cacgaaatga aaatcctgca
     3361 tgctgacctt gccggttccc aaacccgttc caagacctgc cgaaccggcg ttgttcgcgc
     3421 ccatcgacca tgattcgacc tggatttgtt tttcaaagcc gactgcttca gattcaccct
     3481 cgataccgtc aatttttaaa taaaaatcag agtttgccat ttttgttttg ctccttttaa
     3541 taagctggct ataattccag actttcgctc cttaaatata tatacgagtt tttctcgtta
     3601 ccttttatag acggcgcgaa agcctggttt tgccgacatt tcaaatcttt cccaaagaat
     3661 ttttgcgaat ttataccgtc ttacttcgcc ggcggcggca attccgcgac cagacgcaag
     3721 gagacggaaa gctcgtcgag ctgaaagtgc gggcgcagga aggcgaccgc gcgatagcag
     3781 cccggtttgc ccggaatttc cgccacgtcg acgcgcgctt cgcgcaaagg atatttggct
     3841 ttctgctcgg tcggcgcgac gtcgttttcc agaacgtatt tgctgatcca gcgattcaga
     3901 aagatttcgc attccttgcg cgacataaag ctgccgattt tgtcgcgcat catcgatttc
     3961 agataatgag cgaaacgcga aacggcgaaa atatactgca attgcgacga gagacgcgcg
     4021 ttggcgtttg ccgcgtcggt gtcgtatttt ttcgacttgt tcgccgattg ggtagcgaaa
     4081 aacgccgcgt aatcggtgtt tttgcaatgc acgagcggga taaagccgtt gtcggaaaat
     4141 tccttttcgc ggcgatccgt gacggcgacc tcggtcgggc atttcatcgc gatttcgcct
     4201 tcgtcggttt caaacgtgtg cgtcggcaaa tcctgcacca gaccgccgcc ttcgacgccg
     4261 cgaatcgcga cgcaccagcc gtgcatcgag aaagcttcgg tcaggcgcgt gccgagcgcg
     4321 taagcggcgt tgccccacag atattttttg tggtctttgc cgtcgacgtc ttcttcaaaa
     4381 cggaaagatt cggtcggctt ggtcgccgcg ccgtaaggct cgcgcatcaa aacgtgcggc
     4441 agggtcaagc cgacgtagcg cgaatcctcg gattcgcgga agctgcgcca tttcatatat
     4501 tccgtgcggt cgaaaatctt cgaaacgtcg cggacgtccg acatttccga aaaggtgtcc
     4561 cagccgaaca tttccggcga agccgcgctc aggaaaggcg cgtgcgccgt ggcggcgacc
     4621 tgcgagagtt tttcgagcag agccatatcc tgcgggtgat tgccgaattc ataatcgcca
     4681 atcaaagcgc cgaacggagc gccgccgaac gtgccgtatt catcttcgta gattttcttg
     4741 aaaaggctcg actggtcaaa ttccagcgcg cgctcgaaat ctttcaaaag ctcttttttc
     4801 gtgaccgaca taacgcggat tttcagctgc gtactggtca gggaattttt gagcagataa
     4861 tgcagaccgc gccacgagcc ttccagcttt tgaaattctt cgtggtgcat aatttcattc
     4921 atctgcgccg aaatcaggcg gtcaatctcg gcgatgcggg cgttaatcgc gacgtccata
     4981 ttcttggaca tcgtcagctc gccgctcatt acctgattga cgaattcggc aatcatatct
     5041 tttgccgctt ctttctgaaa ttcgtcgcgc gccattctgc cttcggtcaa aatctggtcg
     5101 agcagattcg tctcttgagg cgcatcgacg gttccgacgg cggcggtttc ctgttttgca
     5161 ttctcagcca taatcttatt cctccttcgc tccaccgagc gcatcgctca attgcttttg
     5221 cttgtcggca ttgccgataa cgtcttccag gatgctttcc aatttttcgt tgccgtccat
     5281 cttggaacgt aaatcggcaa gcttctgacg cgcttcgacc agcttgcgaa gcggttcgac
     5341 ctgctggacg atgctgtccg gctcgaaatc ttcgatgccg ttgaatttca gctcaacgcc
     5401 caatttgctg ccgtcgtttt gcagtttatt gtcaaccgaa taggcgaggc gcggcttcat
     5461 tcccgccatc acctgattaa agttgtccgg gtcgagctcg acaaatttgc gttgtttcaa
     5521 agacggcagc ggctcctccg gtttcccgac aaaatcaccc atcacgccga cgacaaacgg
     5581 cagttccttt aattcaatcg ccccgccgat ttccacgtcg taggtaatat tgacacgagg
     5641 cggacgaacc cgctcgagtt tgtgttgtag actttcttta ctaggcatcg cttttgctcc
     5701 tttcgttttt cactgattct tttattaaat taaaatttat actcatccat caggattccg
     5761 tcgaatccgg gtctttcgtg ttaaatccaa gcgtttgacg aatctggaag ataaccgtct
     5821 cgtccttaat aacatcctgc agccagtttt ccagcggcat attgccccat ttgacggctc
     5881 gctgcaccag atacgagacc ggcgagtgcg gctcgttttt cttaaaatat tcggaaacgt
     5941 ccgccaggcg cttcagcgcg tcctgccggc tctgaatcgc gccggtcgca gcgccgacgg
     6001 ttcggacttg aatcgtgccg ttttcgcctt cgacggtttc gaccgtctct tcatcagccg
     6061 cgtccggttc ctgctgccgt ttttcctcca gcagcggttt caggacggtg cgaatgtctt
     6121 cgagcgattt tttcaaattt cgcaagccgg gcatctgatt gcggtcaaat ttttcctcaa
     6181 tcgtgcggtc gagcgcgctg cattccgtcc agcattcttc cagcgtaaaa ttcaattctt
     6241 cgcaaaaagc gcgattcgtc tgcgcctttg ccatccgcca catatcgccg gttttgcggt
     6301 tttcttcggc ggcttgcgcc tttaagagct tcgccttttc ctgctcgtgg taatcgagcg
     6361 cgtcgatatt ttcaggaata tcgaagcttc tggattcttc ccagttcaga tacgtaaagc
     6421 cttcgccgcc ggtaatcggg agcgttttga cggtaatcgc ggtttgcgct tccatccatt
     6481 cgatggcgtt ggcgcgacct tccatatcgc cttcgtcgat ttc
//
LOCUS       DASWNE010000237         2586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_431233, whole genome shotgun sequence.
ACCESSION   DASWNE010000237 DASWNE010000000
VERSION     DASWNE010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431233"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            510..791
                     /locus_tag="VGB68_04835"
     CDS             510..791
                     /locus_tag="VGB68_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048587.1"
                     /translation="MNGLAGMEKFSHLEDKIYLTIEFAKKMREEKEKLERETNSLRHE
                     VSSLLEEKTRLEEKIERLMSERDAIQLKIEAMLDAITVIEPEVSEVLQR"
     gene            801..1151
                     /locus_tag="VGB68_04840"
     CDS             801..1151
                     /locus_tag="VGB68_04840"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEY0048588.1"
                     /translation="MIGNKSKSEQAAQSIRVEIYNQTYNIRSDGDNEYIMRLAEYVDG
                     KMREISSGTLTVDSLKVAILAALHIADEFHQLKRGQEQTDAQLASRSAECAEMLDRVL
                     KQRDVAPQEFEMEQ"
     gene            1604..2386
                     /locus_tag="VGB68_04845"
     CDS             1604..2386
                     /locus_tag="VGB68_04845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEY0048589.1"
                     /translation="MNVLILADVVARPGRRAVTERIQDLREQYKIDIALMNAENVAGG
                     FSITPAIAEELFAAGIDVMTSGNHIFDKHEIIPYIRKNPRLLRPANYPASTPGKGLFI
                     GEVRGHRIAVLNLLGRVFMPPVDEPFRTADECVKAIPEDVKIRIVDMHCEATSEKYAM
                     GWFLDGRVSLVYGSHTHVQTADERILNNGTAYITDVGMTGSYAGCIGMNKEHVIQRFM
                     NIPARRAEHSTDDVWICALVVEIDEETGRANKIERLRLTHVS"
     gene            complement(2405..>2586)
                     /locus_tag="VGB68_04850"
     CDS             complement(2405..>2586)
                     /locus_tag="VGB68_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048590.1"
                     /translation="WLIVNSQLLILNYLTKDTEHQFRIYNLEFTIFPFFIFVQSPKKT
                     KGEEIFTFAFLTFHS"
BASE COUNT          724 a          590 c          646 g          626 t
ORIGIN      
        1 gctcaatcac gattcggctc gaataccgga gcgacgaaag aactctgacc gaacccgacg
       61 ccgaagccgt ccacacgcag attttgcaag ctctcgaacg cgatttaggc gcgaggcaga
      121 gattttaacg agtgcgagcg cgaaatgcgg aagttcgcgt ttcgcgttca tcgtttcaaa
      181 agcgtttccg cctttttcgg ataacgccgc gcgtgaaagc ggcatcttgc ggctgaaagt
      241 ttcatcttgc gggcaaaagt ttaatcttgc gggcaaaagt acacgattgc gtgtaaaagt
      301 gtcatcttgc gcgtgaaagt tcaatcttgc gcgtgaaagt gtcatcttgc cggtaaaatg
      361 tcgtttaacg aaacgggcga aatatattcg cgccgtgccg cgtcttgagg cttgaagttg
      421 aattttttat aaaaacttga gaaaacactt gaagtttttg cgctttctga acataatcag
      481 tttcacaaaa cctttttgtg gagaaaagaa tgaacggtct agccggaatg gaaaaatttt
      541 cgcatcttga agacaaaatt tatctcacca tcgaatttgc taaaaagatg cgtgaagaaa
      601 aggaaaaact cgaacgcgag accaacagcc tgcgtcacga agtaagcagc cttttggagg
      661 aaaaaacgcg gcttgaggaa aaaatcgagc ggctgatgag cgagcgcgac gcgattcagc
      721 tgaaaatcga ggcgatgctc gatgccatca cggtcatcga gccggaagtc tccgaagttt
      781 tgcagcgtta gaaattggat atgattggaa ataaaagtaa aagcgaacag gcggcgcagt
      841 caatcagggt cgagatttat aaccagactt acaacattcg ctcggacggc gacaacgaat
      901 acattatgcg gctggcggaa tacgtcgacg gcaaaatgcg cgagatttct tcgggcactc
      961 tgacggtcga cagcctgaaa gtcgccatcc tcgccgcgct tcacatcgcc gacgaatttc
     1021 accagctcaa acgcgggcag gaacaaaccg acgcccagct tgcctcgcgc agcgccgaat
     1081 gcgccgaaat gctcgaccgc gtgctgaaac aacgcgacgt cgcgccgcag gaattcgaga
     1141 tggaacaatg aagaaaatga aaaatgcaaa gtgcagaatg caaaatggaa caattaattt
     1201 aatttcggaa tcttgaatct taaatcctaa tgtgttgcat tttgcatttt tcgttttgca
     1261 ttttcttcgt ctgtgctaca atcttttcag gcggaagaaa ctactgcaaa gcgcgtgaaa
     1321 aaacgcgatg ataattgagc caacatttga atccttggga gaccgatgcc gttttcagtg
     1381 caatctttca acgttaaaga cttgccggag aactcggctt agatgctttc tcgacggaga
     1441 agcatcggag gtcgcccacc tgttgagaaa caggttcaat tcaagcgttt acacggcttc
     1501 tgcggttctc tttcgctaac attttcagtc gagagtcgag agtcgagagt cgagagaaaa
     1561 taattatctc gacttttaac tttcgactct cgatttattt tttatgaacg ttttgattct
     1621 tgcagacgtt gtcgcgcgtc ccggtcgccg cgccgttacg gagcgcattc aggatttgcg
     1681 cgagcaatat aaaatcgaca tcgctttgat gaacgccgaa aacgtcgcgg gcggtttttc
     1741 catcacgccg gcgattgccg aagagctttt cgccgccgga atcgacgtga tgacttccgg
     1801 caatcatatt ttcgataaac acgagataat tccttatatc agaaaaaacc cgcgcctgct
     1861 gcgtccggcg aattatcccg cttcgacgcc cggcaaaggt ctttttatcg gcgaggtgcg
     1921 cgggcaccga atcgccgttt taaatctttt gggcagagtt tttatgccgc cggtcgacga
     1981 gcctttccgc acggcggacg aatgcgtcaa ggcgattccc gaagacgtga aaatacgaat
     2041 cgtcgatatg cactgcgaag cgacttcgga aaaatacgcg atgggatggt ttctggacgg
     2101 acgcgtctcg ctcgtttacg gctcgcacac gcacgtgcag acagccgacg aaagaattct
     2161 caataacggc acggcataca ttacagatgt cgggatgacc ggcagctacg cgggctgtat
     2221 cgggatgaat aaagagcacg tcatccagcg ttttatgaat attccggctc gccgcgccga
     2281 gcattcgacc gacgatgtct ggatttgcgc gctggtcgtc gagattgacg aggaaaccgg
     2341 cagggcaaat aaaatcgagc gcctgcgcct gacccacgtt tcgtaaaatc acgaaaactc
     2401 aaaatcagga atgaaaagtt aaaaaggcaa aagtgaaaat ttcttcacct tttgtctttt
     2461 tagggctttg cacgaaaata aagaaaggga aaattgtaaa ttctaaattg taaattctaa
     2521 attgatgttc ggtatctttt gttaagtaat ttagaattaa taattgagaa tttacaatta
     2581 gccatc
//
LOCUS       DASWNE010000238         2656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_607510, whole genome shotgun sequence.
ACCESSION   DASWNE010000238 DASWNE010000000
VERSION     DASWNE010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607510"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="VGB68_04855"
     CDS             complement(<1..911)
                     /locus_tag="VGB68_04855"
                     /inference="COORDINATES: protein motif:HMM:NF013216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEY0048591.1"
                     /translation="MKISPARLASFEILCKIEKEKAFSSVLLPLYEEKLEAKDRALCH
                     QLTLGVLRKKIYLDRVVQSFSKTKIAKLDLEVLNALRLGLYQMRFLDKIPDFSAINES
                     VNLVGLAKKTSAKGFVNAVLRRSTREKPELKFADEVERVSVETSHPRWLVENWINQFG
                     HTETEKLAVSNNETPPLVFRLTAKSDEKTAEILQKLGVDLEKSEIAASAWRVKKTGEM
                     LFAYAGEGKIYFQEESSQMVAETINLQPDETFLDVCAAPGSKFTQIQNLKFRIQDSGF
                     RIQNLSVAGDLYEHRLRFLKQSAANQG"
     gene            complement(912..1661)
                     /locus_tag="VGB68_04860"
     CDS             complement(912..1661)
                     /locus_tag="VGB68_04860"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY0048592.1"
                     /translation="MKYQIITFYEFKDLNRIGALEEIKESLKLAMRANSIFGTIILAA
                     EGFNSTVCGESENIKTFINQAENILETNLKYKASRHARIPFRRVHVKIKPEIVTLKRQ
                     VDVEKGIGTHVSAQKWNEIISDSETILLDARNDYEYKVGTFKKAINPQTRKFSELPEF
                     VEKNLDPRRHKKIAMFCTGGIRCEKFAPFVKDLGFEEVYQLEGGILKYLEEIPEAESL
                     WQGECFVFDDRVSVDENLQKGIAPDLSTAGK"
     gene            complement(1661..2593)
                     /gene="fmt"
                     /locus_tag="VGB68_04865"
     CDS             complement(1661..2593)
                     /gene="fmt"
                     /locus_tag="VGB68_04865"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEY0048593.1"
                     /translation="MKIVFMGTPQAAVASLERILRDGHEIVAVYTQPDRPAGRGNKLN
                     ASPVKEFAAQNNLQIYQPTKIKTPEALETFKSHAAEVAVVVAYGRILPESFLRAFPKG
                     AINVHFSLLPKYRGAAPVNWAIVNGEKTTGVTTMQMDAGLDTGGILLQRRIEIGETET
                     AVELMRKLSVIGADLLSETLAMFDELTPQAQAEAQASFAPIMKKEDGLISWMLSAPEI
                     ADRARGFQPFPTAFTFYKEKKLTIWKCQPSNASHQLKTVGEILEAKGDRLLVFCGGDT
                     VLQIEELQIEGKRRMTTRDFLNGVKLQAGEILGR"
BASE COUNT          599 a          689 c          620 g          748 t
ORIGIN      
        1 atgccttgat tcgccgccga ttgtttcaaa aatcgcaggc gatgctcata taaatcaccc
       61 gcgacagaca aattctgaat cctgaatcct gaatcctgaa tcctgaattt taaattctga
      121 atttgcgtaa atttactgcc cggcgcggcg caaacgtcca gaaacgtttc gtccggctga
      181 agatttatcg tctcggcgac catctgcgaa gattcttcct gaaaataaat tttgccttct
      241 ccggcgtaag caaaaagcat ttcgccggtt tttttcacgc gccaggcgct ggcggcgatt
      301 tccgattttt ccaaatcgac gccgagcttt tgcagaattt cggcggtttt ctcgtctgat
      361 ttcgccgtca gacgaaaaac cagcggcggc gtttcattat tcgaaaccgc cagcttttcc
      421 gtctccgtgt gaccgaattg attaatccag ttttcgacca gccagcgcgg atgcgacgtt
      481 tcgacggaaa ctctttcgac ttcgtcggca aattttaatt ccggtttctc gcgcgtggag
      541 cggcgcaaaa cggcgttgac aaaacctttc gccgaagttt ttttcgccag cccgacaagg
      601 ttgaccgatt cgtttatggc ggaaaaatcg ggaattttat cgagaaagcg catttggtaa
      661 agtccgagcc ttaaggcgtt taaaacttcc aaatcgagtt ttgcgatttt cgttttgctg
      721 aacgattgga cgacgcggtc gagatagatt ttttttcgca aaacgccgag cgtcagttgg
      781 tgacacaaag cgcggtcttt cgcttccagc ttttcttcgt aaagcggcag cagaacggac
      841 gaaaaagctt tctctttttc gattttgcag agaatctcga agctcgctaa tctggctggc
      901 gaaattttca tttattttcc cgctgtagac aaatccggcg caattccttt ctgcagattt
      961 tcgtcgaccg aaacccggtc gtcgaaaaca aaacattcgc cctgccagag gctttccgct
     1021 tcgggaattt cttccagata tttcaaaatt ccgccttcca gttgataaac ttcctcgaaa
     1081 cccaaatcct tgacgaaagg cgcgaatttt tcgcagcgaa tgccgccggt gcagaacata
     1141 gcgatttttt tatgccgccg cgggtcgaga tttttttcga cgaattcggg caattcgctg
     1201 aattttcgcg tctgcgggtt tatcgctttt ttaaaagttc cgaccttgta ttcgtaatcg
     1261 ttgcgcgcgt ccaaaagaat ggtttccgaa tcgctgatga tttcgttcca tttttgggcg
     1321 gaaacgtgcg tgccgattcc cttttcaacg tcgacctgtc ttttgagcgt gacgatttcc
     1381 ggtttgattt tgacgtgaac gcgccggaaa ggaatgcgcg cgtgacgcga ggctttatat
     1441 ttcagattcg tttcgagaat attttccgcc tgattgataa atgtttttat gttttcggat
     1501 tcgccgcaaa ccgtcgagtt aaagccctcg gcggcgagaa taatcgtgcc gaagatggaa
     1561 ttcgcccgca tcgccagctt taaggactct ttaatttcct caagcgcgcc gattcggttt
     1621 aaatccttaa actcgtaaaa cgtgatgatt tgatacttca ttatcttccc agaatttcgc
     1681 cggcttgaag tttaacgccg ttcagaaaat cgcgcgtcgt catccggcgc ttgccttcaa
     1741 tctgcaattc ctcgatttgc aaaaccgtgt cgccgccgca aaaaaccagt aaccggtcgc
     1801 ctttcgcttc taaaatctcg ccgaccgttt ttaattggtg actggcgttt gatggctggc
     1861 atttccaaat cgtcagcttt ttttctttgt aaaaagtaaa agccgtcggg aaaggctgaa
     1921 agccgcgcgc gcggtcggca atctccggcg cactcagcat ccagctaatc aagccgtcct
     1981 cttttttcat tatcggcgcg aagctggctt gcgcctcagc ctgcgcctgc ggcgtcagct
     2041 cgtcaaacat cgccagcgtt tccgataata aatccgcgcc gatgacggat aatttccgca
     2101 tcaattcgac tgctgtttcc gtttcgccga tttcaatccg cctctgcaat aaaattccgc
     2161 ccgtgtcgag tccggcatcc atctgcatcg tcgttacgcc ggtcgttttt tcgccgttga
     2221 cgattgccca gttgacgggc gcggctccgc gatatttcgg cagaagcgaa aaatgcacgt
     2281 tgattgcgcc tttcgggaaa gcccgcaaaa aactttcggg caaaattctg ccgtaagcga
     2341 cgacgacggc gacctccgcc gcgtgcgatt taaaagtctc cagcgcttcg ggcgttttta
     2401 tcttcgtcgg ctggtagatt tgtagattat tctgcgcggc aaattctttg acgggcgacg
     2461 cgtttaattt attcccgcgt cccgcgggtc ggtcgggctg cgtataaacg gcgacgattt
     2521 cgtgaccgtc ccgcaaaatt ctttccagcg aagcgaccgc cgcctgcggc gttcccataa
     2581 aaacgatttt catagtcaaa taaaacatct atcataaaga aaaaaggcaa aggcgtaaaa
     2641 tccgtgcctt tgcctt
//
LOCUS       DASWNE010000239         8969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_642893, whole genome shotgun sequence.
ACCESSION   DASWNE010000239 DASWNE010000000
VERSION     DASWNE010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8969
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642893"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /gene="bla"
                     /locus_tag="VGB68_04870"
     CDS             complement(<1..702)
                     /gene="bla"
                     /locus_tag="VGB68_04870"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF033105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B3 metallo-beta-lactamase"
                     /protein_id="HEY0048594.1"
                     /translation="MKNYLCKFGFVLFLSVCFSAFAAAQKLAGDREMNQPVEPFRIIG
                     NVYYVGASDVTSYLITTPRGHILIDAGFEETVPQIKANVAKLGFKIEDVKILLVNHAH
                     YDHCGGLAEIKKLTGARLYASPPDAPVLEDGGMSDFRFGGDKPLFAPVKVDEILKNNQ
                     EIRLGDTVLKSFFTFGHTKGATSWTTEVSENNRRYKVVFSSSVTTLDYSLVDNTKYPR
                     VAEDFAKTFATLKNIK"
     gene            complement(751..2115)
                     /locus_tag="VGB68_04875"
     CDS             complement(751..2115)
                     /locus_tag="VGB68_04875"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEY0048595.1"
                     /translation="MTRQIFACIAFVIAFSTLICAQKTNKSSARPKPEIQKMLREISA
                     KRIEADIRRLAGFGTRNTLSEQNNPARGIGAARDWIYDELNKISAGCGNCLAVEKQTF
                     LQPKANRVPEPTNLTNVVATLRGTVDPERIYVVSGHYDSMCTSPTDAKCDAPGANDDA
                     SGTAAVIEMARVMSKRKFDATIVFMTVAGEEQGLLGAAYFAQQAVEKKLDIEAMFTND
                     IIGGVTTFKTAANRQTVRVFSEGVPSNETEQQANTRRGTGGENDSASRQLARFVKETA
                     DLYSPKFRVLMVYRRDRYLRGGDHIPFLERGFSAVRITETNEDYDHQHQNVRTENGKF
                     YGDTPEYVDFEYVADVARVNAASLARLALAPARPKNVGMVTARLGNDTELKWDAGTEN
                     DLAGYEIVWRDTTSPVWTNSQTIGNVTNFVLKGMSKDNYFFGVRAVDKDGNKSPVSFP
                     RPVR"
     gene            2317..4785
                     /locus_tag="VGB68_04880"
     CDS             2317..4785
                     /locus_tag="VGB68_04880"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0048596.1"
                     /translation="MKFLIILFLLVFPAFVLAQHGGHNQQQIETKTATPDDGLGSIDH
                     PVSTSNQEAQSFFNQGLAYLYGFNHEESIKSFRRAAELDANLAMAYWGIALALGSNYN
                     LTADAAQLKEAYANLQKAIALAPKASEIERAYIDALTGRYSDDPQADQKKLARDYKTA
                     MGELAGKYPDDLDAATLYAESMMNLRPWQLWSPDGKPAEGTLEIVNVLESVLRRNPNH
                     TGANHYYIHAVEASPNPERALASAMRLGLLAPNAGHLVHMPSHIYIRTGDYNEAAKSN
                     ADAIQADRRFMRKTGAQGVYTMMYYNHNIHFLSAASAMNGQYAEALKQARELESNVRP
                     HLKMMPMLEMFSPYPLVTLVRFQKWDEIMKYRQPESDLRITGGFWNFARGLAFAENGS
                     PDEAERELTALREKIKIISPETGFGNSTAHGVLKVAEELLAGEIALSRGDKKTAIELL
                     RKAAASEDAVNYNEPPDWDLPVREWLGRALLINGEYAEAEKVYRADLAKHPRNGRALF
                     GLAEALNKQGKTASARMVRREFEKAWEKADTKLSVENLYQSSNTRAQTWKFGEVKLKT
                     GIRMHYAEQGEAGGEPIIFIHGFTDSWFSYRRILPLLDKKYRVFALDQRGHGDSERPL
                     KGYAMKDFAADVIAFMDAKNIRKAILVGHSMGSFAALQTVLDAPQRIEKLVLIGTATT
                     ARNDVTLDLQKTVNNLKDPIDEKFVRDFQVSTSSQTLPDEFINGVVKQSMKLPARIWQ
                     AALAGVLAENYKPKLGKIKTPTLILWGDRENIFSRGEQDLLKTKIAGSTLKVYAETGH
                     SPHWERPEAFARDLDEFISRPKSE"
     gene            complement(4777..5889)
                     /locus_tag="VGB68_04885"
     CDS             complement(4777..5889)
                     /locus_tag="VGB68_04885"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEY0048597.1"
                     /translation="MKLPVIIFICLLLSFSAAAQKSSKNANDEIARQMTDAAEKGRLD
                     EVKSILGRGADINIAPKERPGWTPLMAAATFGQTELVKYLIARGADVKVKLEDGRTTL
                     YQAAQNKSGEEIIEALLKAGVEIDARTEEKRTALIRAAWMERNAAVKLLLDAGADTKI
                     KDENGWTAFRFACVYGKGETVRHFLDHGANPNEKDEKGETPLMWAGESDRAEALRVLI
                     SAGADVNAKSNAGKTPLMNAAARFYYDEVKILLNAGADARARDKNGSNALMLTAFSVF
                     LRDEFGAHGNGEIRWAGAARIINDLIDAGTDVNARNEDGETALILAVKKGNAHFAEAL
                     LAKGADRDIKNKKGKAAKDYIAALPKYERGEMKKLF"
     gene            complement(5914..7926)
                     /locus_tag="VGB68_04890"
     CDS             complement(5914..7926)
                     /locus_tag="VGB68_04890"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY0048598.1"
                     /translation="MKASVLLLCLLCFTAAISANAQPVWQANLDGKVRFYQTTDFGIM
                     LAGSENSLYAVDGQTGERLWRRQHRGLDETSITPVPATDLILLSLDEGDKSRVEAVDL
                     LSGATLWRSDKVKGDVMQLAVEPEADLLAVVLVKKAKGKPGEELKRSPQIHVLRLSDG
                     EELWKKQLESDVELMPSRFGENPGEIPFTLDNYRPPLMLDGRLYLFYEGATSYDARTG
                     QTGERERFKINEGGLALTESDPVFDEKYIYTSGRGKVRAINRQSGKVEWEAKDLGVTP
                     EMAVVGNVLYVRTGGQFTRIKDGETEEKGSFGVSAIDTKNGKTLWRYKGADKGLTNFV
                     FADAGTILVADKDDLITIDARTGKRISDFEHDVERAQFVLINERGEAVVGGKDEIAAF
                     SIREAETRRRADAEIKLSDSQAFFVKEKSYQMSFFAPPLRVSDSTRQEIWRVKHKAPS
                     RGVFRIVAGIALRAAALYFRYGGLATSALNVVRGANLARTVLSLRWSGLKSRFSSFDL
                     TTLASNSVKNYVTNRISVFGIASRTPNLLNRLNGLQVNIPTASSVRGKIIGGAINRVA
                     PSRGEVQESLLERLDPVRQVEKLSDYFLRRKRLADLRGNYMYFYTDLPEPFDKKGLVG
                     VNVHTGKDARFILVSEPDARFTTDETAGLLYSADGNRLQAFDVLDR"
     gene            complement(8045..>8969)
                     /locus_tag="VGB68_04895"
     CDS             complement(8045..>8969)
                     /locus_tag="VGB68_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048599.1"
                     /translation="DAEPESETIHAEQSNIIPVAPAIPWYRRLFAFPQITFAMGALAL
                     VFAGIIAVTVLQTANDSQNVSVARREEIRETSRGTSGASDDGAAQTVETYSGNTSNST
                     SVNPGITSNSNTSATTTVSNTTSANTTLADKESQPALPRQLPSPEAAKNEPQKPLTLS
                     EAQPVNPPELNKTTETATVAPAPKDAQQRQTGEESKELSLRKREQERAALNVSPKSSD
                     NAPSTLGAQPKKRVSGDKNKADEARSVGGKTFRQIGGVWFDSAYTAQPQIMIRRGSDD
                     YKRLDSGLRSIAESLSGTVIVLWKSKAYRIN"
BASE COUNT         1990 a         2451 c         2372 g         2156 t
ORIGIN      
        1 tttaatattt ttgagcgtcg cgaacgtttt ggcgaaatct tcggcgacgc gcgggtattt
       61 cgtattatcg accagagagt aatcgagcgt cgtcacgctg ctggaaaaaa cgactttgta
      121 tctgcgatta ttttccgaaa cctccgtcgt ccagctggtc gcgcctttag tgtgtccgaa
      181 ggtgaaaaag ctcttcaaaa ccgtatcgcc gagccggatt tcctgattgt tctttaggat
      241 ttcgtcaact ttgaccggcg cgaacaaagg tttgtcgccg ccgaaacgaa aatcgctcat
      301 tccgccgtct tccagaaccg gcgcgtccgg cggactcgcg taaaggcgcg cgccggtcaa
      361 ttttttaatt tccgccagac cgccgcaatg gtcgtaatgc gcgtgattaa cgagcagaat
      421 tttcacgtct tcgattttaa aaccgagttt ggcgacgttc gccttgattt gcgggacggt
      481 ttcttcaaat cccgcgtcga ttaaaatatg cccgcgcggc gtggtaatca gataagaagt
      541 cacgtccgaa gcgccgacgt aatagacgtt tccgataatt ctgaacggtt cgacgggctg
      601 gttcatctcg cggtcgcccg caagcttctg cgcggcggcg aaagcggaaa aacagacgga
      661 cagaaacaat acgaaaccga atttgcaaag gtagtttttc ataatctcca aaatattaag
      721 atgaattccg gtcagtaaac aaaatagatt ttatcttacg gggcgcggaa agctgaccgg
      781 acttttgttt ccgtctttat cgacggcgcg cacaccgaaa aaataattgt ctttcgacat
      841 tcctttcaac acgaaattcg tcacgtttcc gattgtttgc gaatttgtcc agacgggcga
      901 cgtcgtgtcg cgccagacga tttcatagcc cgccaaatcg ttttccgttc ccgcgtccca
      961 tttcagttcc gtgtcattgc ccagtcgcgc cgtgaccatt ccgacatttt tcggacgcgc
     1021 cggagccagg gcgagccgcg ccagactggc ggcattgacg cgcgcgacat cggcgacgta
     1081 ttcaaaatcg acgtattcgg gcgtgtcgcc gtaaaatttc ccgttttccg tgcggacgtt
     1141 ttggtgctgg tggtcgtaat cttcgtttgt ttccgtgatg cgaacggcac taaagccgcg
     1201 ttcgagaaac gggatgtggt cgccgccgcg caaataacgg tcgcggcggt aaaccatcaa
     1261 cacgcggaat ttcggcgaat ataaatcggc tgtttccttg acgaatcggg cgagctggcg
     1321 cgaagccgaa tcgttttcgc cgccggttcc gcgtcgcgta ttcgcctgct gctcggtttc
     1381 gttcgacgga acgccttccg aaaatacgcg gacggtctga cggtttgccg ccgttttaaa
     1441 agtcgtcacg ccgccgatga tgtcgttggt aaacatcgct tcgatgtcca gttttttctc
     1501 gaccgcttgc tgcgcgaaat acgccgcgcc caaaagtccc tgttcctcgc ctgccactgt
     1561 cataaaaaca atcgtcgcgt cgaattttcg tttcgacatc acgcgcgcca tttcgatgac
     1621 ggcggcggtg ccggaagcgt cgtcgttggc tcccggcgcg tcgcatttcg cgtcggtcgg
     1681 cgaagtgcac atcgaatcgt aatgaccgga aacgacgtaa attctttcgg ggtcgaccgt
     1741 gcctctgagc gtggcgacga cgttcgtcag atttgtcggt tcgggaacgc gattggcttt
     1801 cggctgtaag aaagtttgtt tttcgaccgc cagacaatta ccgcaaccgg cgctgatttt
     1861 gttcaattca tcgtaaatcc aatcgcgcgc cgcgccgatt ccgcgcgccg ggttgttctg
     1921 ctcggaaagc gtgtttcgcg tgccgaaacc ggcgagcctg cggatgtcgg cttcgatgcg
     1981 ttttgccgaa atctctcgca gcattttttg aatttccggt tttggtctcg cgctcgactt
     2041 atttgttttc tgagcgcaga ttagcgtcga aaaagcgatg acgaaagcga tgcaggcgaa
     2101 tatttgtctg gtcatataaa ttatggtttc cggtcggttt gttttataaa tgtaatgcta
     2161 aagcgcgcgc cgcgcaatgg aaaaccggca atcaaaaagt cttcgacgcg ccgtcgccgg
     2221 aaaatttcaa aaaaaatgtc cgctcccgga cagttttttc gtcttttaat gcaggtgaag
     2281 tttaccggct tttaatcttt ggaaaaggag atttatatga aatttttaat cattttattt
     2341 ttgcttgtgt ttccggcttt cgttttggct caacacggcg gacacaatca gcagcagatc
     2401 gaaacaaaaa cggcgacgcc ggacgacggg ctgggcagca tcgaccaccc ggtctcgacc
     2461 agtaatcagg aggcgcaaag cttttttaat cagggcttgg cgtatcttta cggctttaat
     2521 cacgaggaat cgataaagtc gttccggcgc gccgccgagc tcgacgcaaa tctggcgatg
     2581 gcgtattggg gaatcgcgct cgcgctcggc tcaaactaca atctgaccgc cgacgccgcg
     2641 cagctcaaag aagcctacgc gaatttgcag aaagcgattg cgctcgcgcc gaaagcgtcg
     2701 gaaatcgagc gcgcttacat cgatgcctta accggacgct attcggacga tccgcaagcc
     2761 gaccagaaaa agctggcgcg cgattacaaa acggcgatgg gcgaactcgc cggaaaatac
     2821 ccggacgatt tggatgcggc gacgctttac gccgaaagta tgatgaatct gcgcccgtgg
     2881 cagctctggt cgccggacgg caaacccgcc gaaggaactc tggaaatcgt caatgttctg
     2941 gaaagcgttt tgcggcgtaa tccgaatcat accggcgcca atcattatta cattcacgcc
     3001 gtcgaagcca gcccgaatcc cgaacgcgcg ctggcgagcg cgatgcgtct cggcttgctc
     3061 gcgccgaatg ccggtcatct cgtccatatg ccttcgcaca tttacatccg caccggcgat
     3121 tacaacgagg cggcgaaatc gaacgccgac gccattcaag ccgaccgccg gtttatgcgg
     3181 aaaaccggcg cgcagggcgt ttacacgatg atgtattaca accacaacat ccattttctc
     3241 tccgccgcca gcgcaatgaa cgggcaatac gccgaagccc tcaaacaggc gcgcgagctt
     3301 gaatcaaacg tccgcccgca cctgaaaatg atgccgatgc tcgaaatgtt ttcgccgtat
     3361 ccgctggtca cgctcgtccg ttttcagaaa tgggacgaga ttatgaaata ccggcagccg
     3421 gaaagcgatt tgcgcattac gggcggattc tggaatttcg cgcgcggtct ggcgtttgcc
     3481 gaaaacggga gcccggacga agccgaaagg gaactaacgg cgctgcgcga aaaaataaaa
     3541 atcatttctc cggaaaccgg attcggcaac agcacggcgc acggcgtttt aaaagtcgcg
     3601 gaagaattgc tcgcgggcga aatcgccctg tcgcgcggcg ataagaagac ggcgattgag
     3661 cttttacgaa aagccgccgc gtccgaagac gcggttaatt ataacgagcc gcccgattgg
     3721 gatttgccgg tgcgcgaatg gctgggcagg gctttattga taaacggcga atacgccgaa
     3781 gccgaaaaag tttatcgcgc cgatctcgcc aaacacccgc gcaacgggcg cgctttgttc
     3841 ggcttagccg aagccttaaa caagcagggc aaaaccgctt cggcgcggat ggtgcggcgc
     3901 gagtttgaaa aagcctggga aaaagccgat acgaaactga gcgtcgaaaa tttgtatcaa
     3961 tcttcaaaca cgcgtgcgca gacgtggaaa ttcggcgagg taaagctgaa aaccggaatc
     4021 agaatgcatt acgccgagca gggcgaggcg ggcggcgagc cgattatttt tattcacggc
     4081 tttacggatt cgtggttttc atacagacgc atcctgcctc tgctcgataa aaaatatcgc
     4141 gtcttcgcgc tcgaccagcg cgggcacggc gattccgaac gacccttaaa aggctacgcg
     4201 atgaaagatt tcgccgccga tgtcatcgcg tttatggacg cgaaaaacat caggaaagcg
     4261 attctcgtcg ggcattcgat gggaagtttc gcggcgctgc agaccgttct ggacgcgccg
     4321 cagcgaatcg aaaaactcgt tcttatcggg acggcgacga ccgcgcgcaa cgacgtcacg
     4381 ctggatttac aaaagacggt taataatctg aaagacccga ttgacgaaaa attcgtccgc
     4441 gattttcagg tcagcacgag ctcgcaaacc ttgcccgacg aatttattaa cggtgtggtt
     4501 aaacaaagta tgaaactgcc cgcgcgcatc tggcaggcgg cgctggcggg cgtgctcgcc
     4561 gagaattaca aaccgaagct cggcaaaatc aaaacgccga cgctgatttt gtggggcgac
     4621 agggaaaata ttttctcgcg cggcgagcag gatttgctca aaacgaaaat cgccggttcg
     4681 actttgaaag tttacgccga aacgggacac tcgccgcatt gggaacgacc ggaagccttt
     4741 gcgcgcgatt tggacgagtt tatcagccgc ccgaaatcag aataattttt tcatttctcc
     4801 gcgttcatat ttgggcagag cggcaatgta atcttttgcc gccttgcctt ttttattttt
     4861 tatatcgcgg tcggcgcctt tcgctaaaag cgcttcggca aaatgcgcgt ttcccttttt
     4921 gaccgccagt atcaaggcgg tttcgccgtc ttcgttccga gcgttgacgt ccgttccggc
     4981 gtcgattaaa tcgttgataa ttcgggcggc tcccgcccag cgtatctcgc cgttgccgtg
     5041 cgctccgaat tcatcgcgca gaaagacgct gaaagccgtc agcatcaaag cgttggagcc
     5101 gtttttatcc cgcgcccgag cgtcggctcc ggcatttaag aggattttta cctcgtcata
     5161 ataaaaacgc gcggcggcat tcattaaagg agttttgccg gcgttgcttt tcgcgttgac
     5221 gtcggctccg gcgctgatta aaacgcgtaa agcttccgcc cggtcagact ctccagccca
     5281 cattaaaggc gtctcgcctt tttcatcttt ttcgttcggg tttgcgccgt ggtcgagaaa
     5341 atgcctgacg gtttcgccct tgccgtaaac gcaggcgaag cgaaacgcgg tccagccgtt
     5401 ttcgtctttt attttcgtat ctgctccggc gtctaaaagc agtttcaccg ccgcattgcg
     5461 ctccatccac gccgctctga ttaaagccgt gcgcttttcc tcggtccggg catctatttc
     5521 cacgcccgct ttcaataaag cttcgataat ttcttcgccg cttttgttct gggcggcttg
     5581 ataaagcgtc gttctgccgt cttccagttt tactttgacg tcggcgccgc gcgcgattaa
     5641 atattttacg agttcagttt gcccgaaggt tgccgccgcc attaacggcg tccagcccgg
     5701 acgctctttc ggagcgatgt ttatgtccgc gccgcgcccg agtattgatt ttacttcgtc
     5761 cagcctgcct ttttcagccg cgtccgtcat ttgacgcgcg atttcgtcgt tcgcattttt
     5821 cgacgatttt tgagccgcag ccgaaaaaga cagcagcagg caaatgaaaa taattaccgg
     5881 aagcttcata aaaaaaactt taattaaaag attctatcta tccaaaacgt caaacgcctg
     5941 caggcgattg ccgtccgccg aataaagcaa gccggcggtc tcgtcggtcg taaaacgcgc
     6001 gtcgggctcg gagacgagaa taaaacgcgc gtcttttccg gtgtgaacgt taacgccgac
     6061 caagcctttt ttatcaaacg gctcgggcaa atcggtgtag aaatacatat aatttccgcg
     6121 caggtcggcg agccgcttgc ggcgcaggaa ataatcggaa agcttttcga cctggcgcac
     6181 cgggtcgagg cgctcgagca ggctttcctg cacctcgccg cgcgaaggcg cgacgcgatt
     6241 gattgcgccg ccgatgattt ttccgcgcac gctcgaagcg gtcgggatat tgacctgcaa
     6301 accgttcaat cgatttaaaa gattcggcgt ccgcgaagcg attccgaaaa cgctgatgcg
     6361 gtttgtcacg taatttttta cggaattcga ggcgagcgtc gtcaaatcaa acgaggaaaa
     6421 acgtgatttc aagcccgacc agcgaagcga caaaaccgtg cgcgccagat ttgctccgcg
     6481 cacgacgttt aaagccgaag tcgccagccc gccgtaacga aaataaagcg cggcggcgcg
     6541 cagcgcgatg cccgcgacaa tgcggaaaac gcctcgcgaa ggcgctttgt gtttgactcg
     6601 ccagatttcc tgacgcgtcg aatcggagac gcggagcggc ggagcgaaaa aactcatttg
     6661 atatgatttt tccttaacaa aaaaagcttg agaatcggaa agcttaatct ccgcgtctgc
     6721 gcgtctgcgc gtctccgcct ctcgaatcga aaacgccgca atctcatctt ttccgccgac
     6781 gaccgcttcg ccgcgctcgt ttatcaaaac gaactgcgcc cgctcgacgt cgtgttcaaa
     6841 atccgaaatt cgcttgcccg tgcgcgcgtc aatcgtgatt aaatcgtctt tgtcggcgac
     6901 cagaatcgtg ccggcgtcgg cgaaaacaaa attcgtcaag cctttgtccg cgcctttgta
     6961 acgccacagg gttttgccgt ttttcgtgtc gatcgccgaa acgccgaaac tgcctttttc
     7021 ttccgtttcg ccgtctttga tgcgcgtgaa ctgcccgcct gttctgacgt ataaaacgtt
     7081 tccgacgacc gccatttccg gcgtcacgcc caaatccttc gcttcccatt cgacttttcc
     7141 gctttgccgg tttatcgcgc ggactttgcc tctgccggaa gtataaatgt atttttcatc
     7201 aaaaaccggg tcggattccg tcaaagccaa gccgccttcg ttgattttaa atcgctcgcg
     7261 ctcgcctgtc tgtccggtgc gcgcatcgta gctggtcgcg ccttcgtaaa ataaataaag
     7321 cctgccgtcg agcatcaaag gcggtcgata attatccaga gtgaaaggaa tctcgcccgg
     7381 attttcgccg aagcgcgaag gcatcagctc gacgtcggat tcaagctgtt ttttccaaag
     7441 ctcttcgccg tccgagaggc gtaaaacgtg tatttgcggc gagcgtttta attcttcgcc
     7501 cggctttcct ttcgcttttt tgaccagcac gacggcgagc aaatccgctt cgggctcgac
     7561 cgccaactgc atcacgtcgc ctttgacttt atcgctgcgc cagagcgtcg cgcccgaaag
     7621 caaatcgacg gcttcgacgc gcgatttgtc gccttcgtcc agcgaaagca aaatcaaatc
     7681 ggtcgccgga accggcgtaa tcgaagtttc gtccaagcct ctgtgctgcc tgcgccagag
     7741 cctttcgccg gtttgcccgt cgacggcgta aagcgaattt tcgctgcccg ccagcataat
     7801 gccgaaatcg gtggtttgat aaaatcgaac tttgccgtcg aggtttgcct gccagaccgg
     7861 ctgcgcattt gcggaaatcg ccgccgtaaa acacaaaaga cacaaaagta aaacagaagc
     7921 tttcatacga ttattatcca caaaaaaaca caaaataaca ctaaatttca gtgttatctt
     7981 gtgtgatgcg cgtcggtcac aaaaagtttc tacgcagaat gaccgcaaaa aaatttcaca
     8041 aacctcaatt aattcgatag gctttgcttt tccacaaaac gatgaccgtc ccgcttaagc
     8101 tttcggctat cgagcgcaag ccggaatcga ggcgtttgta atcgtccgag ccgcgccgaa
     8161 tcataatctg cggctgcgcc gtgtaagccg aatcgaacca gacgccgccg atttgacgga
     8221 acgttttgcc gccgaccgag cgcgcttcat cggctttatt tttgtctccg gaaacgcgtt
     8281 ttttcggctg cgcgcccagc gtcgacggcg cgttgtcaga tgatttcggc gagacgttaa
     8341 gagcggcgcg ctcttgctcg cgctttctca acgaaagttc ttttgactct tctccggttt
     8401 gccgctgttg ggcatctttc ggggcgggcg cgacggtcgc cgtttccgtt gttttattca
     8461 attccggcgg gtttacgggc tgcgcttcgc tgagcgtcaa aggcttttgc ggttcgtttt
     8521 tcgcggcttc gggcgacggc agctggcgcg gcagagcggg ctgagattct ttgtcggcta
     8581 aagtcgtatt cgcgctggtc gtatttgaaa cggtggtcgt cgccgacgta ttcgaattgc
     8641 tcgttatgcc cggattgacg ctcgtcgagt ttgacgtatt gcccgaataa gtttcgaccg
     8701 tctgcgccgc gccgtcgtcg ctggctccgc tcgtgcctct cgatgtttcg cgtatttctt
     8761 ctcttctggc gaccgataca ttttgagaat cgtttgccgt ttgcagcacc gtcacggcaa
     8821 taattccggc gaagaccagc gccagcgcgc ccatcgcaaa agtgatttgc ggaaacgcga
     8881 aaagccgccg ataccaggga atcgcgggcg cgaccggaat aatattgctt tgttcggcgt
     8941 gaattgtttc actttcaggc tcggcatcg
//
LOCUS       DASWNE010000240         3572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_255026, whole genome shotgun sequence.
ACCESSION   DASWNE010000240 DASWNE010000000
VERSION     DASWNE010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255026"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2662)
                     /locus_tag="VGB68_04900"
     CDS             complement(<1..2662)
                     /locus_tag="VGB68_04900"
                     /inference="COORDINATES: protein motif:HMM:NF015177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048600.1"
                     /translation="MTTENDLNAERKRLEQARLREKDWKRWGCYVSERAWGTVREDYS
                     ADGAAWDYFPFDAARKRAFRWSEDGIAGVCDRKQGICFAVAFWNERDPILKERLYGVG
                     GNEGNHGEDVKEYYFYLDSTPTHSYMKFLYKYPHAEFPYERLKNENLRRDKSQPEFEL
                     ADTGIFDEDKYSDVFIEYAKADVEDICIKITAVNRSGEHAPLHVLPIVWFRNVWSWSA
                     DAEKPHLEKAETAAENLSVVKLEHNELGARRLYCAGAPDLLFTENETNNKKLFGAPNV
                     SEYVKDGIEDFVVNRKTEAVNPNQTGTKAAAHYVFNLAPASSETIYLRFTNQELSGGE
                     TFVDDCEAIFRQRIIEADEFYEEIIPGNLSDDAKNVMRQSFAGMLWSKQFYHYVVKQW
                     LDGDAKQPAPPEKRENGRNSKWTHLYNSDVVSMPDKWEYPWYAAWDLAFHCIPLAIID
                     ADFAKRQLILMLREWYMHPNGQIPAYEWKFEDVNPPVHAWAALRVYKIEARRRGTPDR
                     AFLEKIFHKLLLNFTWWVNRKDFEGNNVFEGGFLGLDNIGIFDRSAPLPFSAQVEQSD
                     GTSWMAMFSLNMLAIALELAKEDKVYEDVASKFFEHFVYIADAMNNIGEEQTELWNER
                     DGFYYDVLHLDGKQIPLRLRSMVGLIPLFAVETLEEAWLDELPDFKRRMDWFLEHRPD
                     LTGSIECGQLPGRKNRRILALVNRERLKRVLRVMLSENEFLSDYGIRSVSRWHKSNPY
                     ILRLGDEQYRLDYEPAESRSGTFGGNSNWRGPVWFPVNYLLIEALQKFDFYYGDDFKV
                     EFPTGSGKMLTLWQVSQELEKRLSQIFLKDAEGRRAVYGKIEKFQSNENWRNYILFYE
                     YFHGDNGSGLGASHQTGWTGLIGKILQQIGE"
     gene            2874..>3572
                     /locus_tag="VGB68_04905"
     CDS             2874..>3572
                     /locus_tag="VGB68_04905"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0048601.1"
                     /translation="MRKDLFSANERPQSENASIFGAGLFDRSPKGLKLMTNEKLTEVF
                     SEIQLKLDNGQSSAAEKIIDETLGSFSLKLDEKIRLYSQLSFVYETQGRYADALAALR
                     RFEDESVLSRLEAAIRVSLITQIAVAYNNAGDYPKAVALLKSTLEEAEAGDMRHLFGG
                     IYTALARVYRKLNEFPIARDNAEKGLKYFREIGDWRGMAESYHLIAQSLQQGGNSEKS
                     LEFYNQAIKIIGSRS"
BASE COUNT          850 a          971 c          877 g          874 t
ORIGIN      
        1 attctcctat ctgctgcaaa attttgccaa tcaagcccgt ccagcccgtc tgatgactcg
       61 cgcccagacc cgaaccgtta tcgccgtgaa aatattcata gaaaaggatg taattgcgcc
      121 agttttcgtt cgattgaaat ttttcgattt ttccgtagac ggcgcgcctg ccttccgcgt
      181 ctttcaaaaa tatctgcgac aggcgttttt cgagttcctg cgaaacctgc cagagcgtca
      241 gcatttttcc cgaaccggtc ggaaattcga ctttaaaatc gtcgccgtaa taaaaatcga
      301 atttttgcag agcttcaatc aaaagataat tgaccggaaa ccagacggga ccgcgccagt
      361 tcgaattgcc gccgaaagtt ccgcttctgg attccgccgg ctcgtaatcc agacgatatt
      421 gctcgtcgcc gagccgcagg atatacgggt tcgatttatg ccagcgcgag acggagcgaa
      481 tgccgtaatc cgacaaaaac tcgttttccg aaagcattac gcgcaaaacc cgcttcaagc
      541 gctcgcgatt gaccagcgcc agaatgcgtc gattttttct gcccggcagc tgcccgcatt
      601 caatcgagcc ggtcaaatcg gggcgatgct ccagaaacca gtccatccgc cgcttgaaat
      661 ccggcagttc gtcgagccac gcttcctcga gcgtttcgac ggcgaaaagc ggaatcagcc
      721 cgaccatcga gcgcagtctt agaggaattt gctttccgtc cagatgcagc acgtcgtaat
      781 aaaaaccgtc gcgctcgttc cagagctcgg tctgctcttc gccgatgttg ttcatcgcgt
      841 cggcgatata aacgaaatgc tcgaaaaatt tgctcgccac gtcttcgtaa accttgtctt
      901 ctttagccag ttcgagcgcg atggcgagca tattcaggct gaacatcgcc atccacgaag
      961 ttccgtccga ttgctcgacc tgcgcgctga acggcagcgg cgcgctgcgg tcgaaaatgc
     1021 cgatgttgtc gagcccgaga aagccgcctt cgaaaacgtt gttgccttca aaatccttgc
     1081 gattgaccca ccaggtgaaa ttcaaaagca gtttatgaaa gattttttcg agaaacgcgc
     1141 ggtcgggcgt tccgcgacgg cgcgcctcga ttttataaac gcgcagcgcc gcccacgcgt
     1201 gaaccggcgg attgacgtct tcaaatttcc actcgtaagc gggaatctgc ccgttcgggt
     1261 gcatatacca ttcgcgcagc atcaatataa gctggcgctt ggcaaaatcg gcgtcgataa
     1321 tcgcgagcgg aatgcagtga aacgccaaat cccaagccgc ataccaggga tattcccatt
     1381 tgtccggcat cgagacgacg tccgagttat acaaatgcgt ccatttcgaa tttctgccgt
     1441 tctctctttt ttccggcggc gcgggctgct ttgcgtcgcc gtcgagccac tgtttgacga
     1501 cgtaatgata aaactgtttt gaccaaagca ttccggcaaa cgattgacgc atcacgtttt
     1561 tcgcgtcgtc ggacaaatta ccgggaatga tttcctcgta aaactcgtcg gcttcaataa
     1621 tgcgctgccg gaaaatcgct tcgcagtcgt cgacgaaagt ttcgccgccg cttaattcct
     1681 gatttgtaaa tcgcaggtaa attgtttcgc tcgatgcggg cgctaaattg aaaacgtaat
     1741 gcgccgccgc ttttgttccc gtctgattcg ggttgacggc ttccgttttt cggttgacga
     1801 cgaaatcctc gatgccgtcc ttgacgtatt cggaaacgtt gggcgcgccg aaaagttttt
     1861 tattgttcgt ttcgttttcg gtaaaaagca ggtcgggcgc gcccgcgcag taaagccggc
     1921 gcgcgcctaa ttcgttgtgt tcgagcttga cgacggaaag attttccgcc gcggtttccg
     1981 ctttttccag atgcggcttt tccgcgtcag ccgaccacga ccagacgttg cgaaaccaga
     2041 caatcggcaa aacgtggagc ggcgcgtgtt cgccggagcg attaacggcg gtgattttta
     2101 tgcaaatatc ttcgacgtcg gcttttgcgt attcgatgaa aacgtccgaa tatttgtctt
     2161 cgtcaaaaat tccggtgtcc gccaattcaa attccggctg cgatttgtcg cgccgtaaat
     2221 tttcgttttt gagccgttca tacggaaact cggcgtgcgg gtatttatac aaaaatttca
     2281 tataggaatg cgtcggcgtc gagtccagat aaaaataata ttctttgacg tcttcgccgt
     2341 gatttccctc gttgccgccg acgccgtaga gcctttcttt taaaatcggg tcgcgctcgt
     2401 tccaaaaagc gacggcgaaa cagattccct gcttgcggtc gcaaacgccg gcgatgccgt
     2461 cctcgctcca gcgaaaagct cgctttcgcg ccgcgtcaaa cggaaaataa tcccacgccg
     2521 cgccgtccgc cgaataatct tcgcggacgg ttccccacgc ccgctcgctc acgtaacagc
     2581 cccagcgctt ccagtctttt tcgcgtaagc gcgcctgttc cagacgttta cgctcggcat
     2641 tcaaatcatt ttcggtcgtc ataaatttct taaaagaatg tcatatttaa cgaatttaag
     2701 tccagcattt atatttcgcg ttaaaaatta atggacgaag cgcgaaaact ctggtaaatt
     2761 cgcagaaaat acggttttct tttttccgtc gcccgaggct cgcggtttga aattttttat
     2821 aatgcattac tgaaaaacag ccctaattaa aagtatgtag attttaccgc gaaatgagaa
     2881 aagatttgtt ttcagccaac gagcgcccgc agtcggaaaa tgcttctata ttcggagccg
     2941 ggctattcga ccgttcgccg aaaggtttaa aactgatgac caacgaaaag ctcaccgaag
     3001 ttttcagcga aatccagctg aagctggaca acggacagtc cagcgcggcg gaaaaaatta
     3061 tcgacgaaac cctgggctcc ttttcgctca agctcgacga aaaaattcgg ctttattcgc
     3121 aattgtcctt tgtttatgaa acgcagggac gctacgccga cgcgctcgcc gcgcttcgcc
     3181 ggtttgaaga cgaaagcgtg ctgagccgcc tcgaagcggc aatccgagtc tcgctgatta
     3241 cgcaaatcgc cgtcgcctac aacaacgcgg gcgattaccc gaaagccgtc gcgcttttaa
     3301 aatcgacgct cgaagaagcc gaagcgggcg atatgcgaca tcttttcggc ggcatttaca
     3361 cggcgctggc gcgcgtctac cgcaagctga acgaatttcc catcgcgcgc gacaacgccg
     3421 aaaagggctt gaaatatttt cgcgagatcg gcgactggcg cgggatggcg gaaagctatc
     3481 atttaatcgc ccaatcactg caacagggcg gcaacagcga aaaatcgctg gaattttata
     3541 atcaggcaat caaaatcatc ggctcgcgct cg
//
LOCUS       DASWNE010000241         6393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_7691, whole genome shotgun sequence.
ACCESSION   DASWNE010000241 DASWNE010000000
VERSION     DASWNE010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6393
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7691"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(263..769)
                     /locus_tag="VGB68_04910"
     CDS             complement(263..769)
                     /locus_tag="VGB68_04910"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0048602.1"
                     /translation="MSQTEFTIRPVSLEDLPFLREMLFEAAAVSDEIRALGMEKALVL
                     PFIAHILEHFGRRGDFGFVAGIDNKILIGAIWARLFPEQAKGYGFVSAEIPELAVAVA
                     PEFRGRGAGTRLLEELIKEARNLNFSALSLSVDRRNPALKLYERLGFTDAGVSKKTDS
                     SLTMILYL"
     gene            889..1533
                     /locus_tag="VGB68_04915"
     CDS             889..1533
                     /locus_tag="VGB68_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048603.1"
                     /translation="MQFYILKKAFGIYLSMIFMLLVSACAEGQDFSRYDLSTDEGVSA
                     AREAKLGRKLPDYSKGCVKRPKELPEIILVGTFAHDRGCMLEGAFVKKYFLSRQENLS
                     GQALENLGWRKADPTGREEIALRWTRFGLLAFGGAPIIKANEDFDGREFHSPESTTGE
                     QEGVVNVVLWTRRPAGMRCETGYERLSFVFAADGSLRERKSIDNFTIPCKRNQN"
     gene            complement(1641..3452)
                     /gene="typA"
                     /locus_tag="VGB68_04920"
     CDS             complement(1641..3452)
                     /gene="typA"
                     /locus_tag="VGB68_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEY0048604.1"
                     /translation="MITKEKIRNIAIIAHVDHGKTTLVDAMLQQSGTYRDNETVVDRV
                     MDSMDLERERGITIMAKNTSVRYGDYKINIVDTPGHADFGGEVERVLKMVDGIVLLVD
                     AAEGCLPQTRFVLRKALELKLPAIALVNKIDRHDARPEEVVDEIYDLFIDLGADDRQI
                     EFPILYSISRDGVAKKALADESKSLKPLFEQIIETVPAPHELRTDTLQLLVANIDYNP
                     FVGRLAIGRIYSGEIAKNQEVLVAKRDGSTQKTRVKELYVFESLDRKTVESAGVGEIV
                     ALAGFDDINIGETITLPDNPKPLPLINVDEPTIAMIFGVNTSPFSGTEGKFVTSRQIK
                     ERLEKELLGNVALRVAETDSPDSFRVSGRGELQLSILIEMMRREGFELQVSKPEVITR
                     RHEKTGELQEPIEQVVIDVPEEFIGVVTEALGRRKGQMSKMINNGTGRVRLEYEVPSR
                     GLIGFRGEFLTETKGTGLLNTLFLRFDKWQGEMKGRSTGSLVSDRMGEATTYALFNLQ
                     ERGSLFIKPQIKVYEGMIVGENARAVDLDVNAIKEKKLTNMRASSADEAMRLVPVKDL
                     SLEQALEFIADDELVEVTPKSIRLRKRVLKANERPKK"
     gene            complement(3667..5853)
                     /locus_tag="VGB68_04925"
     CDS             complement(3667..5853)
                     /locus_tag="VGB68_04925"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEY0048605.1"
                     /translation="MKEKKLFSRRWLSVFTTLAVLAWISSPLISFITASANNPPNPPQ
                     LKLQASLSGTPINNLSPFGIAEYKLESNNLRKFELEIFSVNLPAGTILDVYINNNLVG
                     QITVNSSRTGELELRSDHQQNTPVIQSGDVITVRQGATNILSGTFTGAGSPTPTVSPT
                     GSPTACADGPLFATLTGAPVNGQTPRGFAQFRDSNNNQLKVFVRFLNLPDNTTLDVFV
                     GGVDVGDIQLRDNGNGQVFIDTQMNITPGTVITIRNGSTTVLSGTFFCANPSPSPSPS
                     VSPTVSPTVSPTVSPTVSPTVSPTASPTVSPTVSPTASPTVSPTPSPSPNQSRLFRAK
                     LRGNQVVPQVNTQARGFAFALLNEAGTQLQVSAGFFGLSSNQTTASINCPALPGQNAP
                     AVFNLGTVGGRSGFLPVQTFAVTAEQAAQLRTGLCYIVIGSQNNPNGEIRGQLRAVHI
                     GGDFEGDGLFDLSVFRPSDGNWYSLNSSDGFFRAQRFGLAGDKALSGDYDGDGIGDLS
                     VFRASGGSGYWYIQRSSDGGFSAEQWGSSGDIPVAADFDGDDRSDLVVFRPSDGTWYI
                     RYSSNGTYSAIRWGLNGDIPVVGDYDGDGQNDIAVFRPSNGTWYINRSSDGALGAFRW
                     GAGGDIPQSGDFDGDGVNDIAVFRPSDGNWYILRSIDGDYRIIRFGLSGDIPVAGRFD
                     SDRKTDIAVFRPTDGTWYVLRSENNDFVAVKFGQNGDQPIGMNIAP"
     gene            complement(6119..>6393)
                     /locus_tag="VGB68_04930"
     CDS             complement(6119..>6393)
                     /locus_tag="VGB68_04930"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY0048606.1"
                     /translation="VINNIEVLPPSGFDNSIRRQILRSFSDKGLYRYLWEPNPSVRII
                     VQGGRVELEGYVRNRGDYDLMNILANGVSNVFQVRNNLIVEKEDVR"
BASE COUNT         1531 a         1662 c         1638 g         1562 t
ORIGIN      
        1 cgaaaacgat aaaacgataa aacgataaaa cgaaaaacga aaaactttaa ttttcaaact
       61 gttcgttttc cgctttccgt ttcctttctg ctttgcacaa aaataaagaa aatcgagaaa
      121 aatttcgatg gctaattgta aattctaaat tattaattct aaattacttc acaaaagata
      181 ccgaacatca atttagaatt tacaatttac aatttacaat tttccctttc tttatttttc
      241 tgcaaagcct ttcctttctg ctttacaaat acagaatcat cgtcagcgaa gaatccgttt
      301 tctttgaaac gccggcgtcc gtgaaaccta atctctcata aagcttgaga gccggattgc
      361 ggcggtcgac cgaaagactg agcgccgaaa aattcagatt tcgcgcttct ttaatcaatt
      421 cttccagcaa gcgcgttccc gcgccgcgcc cgcgaaattc aggcgcgacg gcgacggcga
      481 gttccggaat ttccgccgaa acaaagccgt agcctttcgc ctgttcggga aacaggcgcg
      541 cccagattgc gccgattaaa attttattgt caatcccggc gacaaatccg aaatcgcctc
      601 gccgcccgaa gtgttccaga atgtgcgcga taaaaggcag gacgagcgct ttttccatcc
      661 ccagcgcgcg gatttcatcg ctgaccgccg ccgcttcaaa aagcatctcg cggagaaaag
      721 gcaaatcttc gagcgacacc gggcgaattg taaattcggt ttgactcatc cttggtttgc
      781 gggcttcccg tatcttccgc aaattttgcc cgcattgcaa gtgtcgccgc aaatttcgtt
      841 caagttaagg atttatttga ttgatattga cagtcgaggt aaaagtaaat gcaattttat
      901 attttgaaaa aggcttttgg aatttattta tcgatgattt ttatgctgct ggtttccgcc
      961 tgtgcggaag gtcaggattt ttcgcgctac gacctttcga ccgacgaagg cgtcagcgcc
     1021 gcgcgcgagg cgaagctcgg cagaaaactg cccgattaca gcaaaggctg tgtcaaaaga
     1081 ccgaaggagc tgccggaaat tattctggtc ggaacctttg cgcacgaccg cggatgtatg
     1141 ctcgaaggcg cgttcgtcaa aaaatatttt ctttcgcggc aggaaaatct ttccggacaa
     1201 gcgctggaaa atctcggctg gcgcaaagcc gacccgaccg ggcgcgaaga aatcgcgctc
     1261 agatggacgc gattcgggct tcttgcgttc ggcggcgctc caatcatcaa agcgaacgaa
     1321 gatttcgacg gcagggaatt tcactcgccg gaatcaacga cgggcgagca ggaaggcgtc
     1381 gtcaacgtcg tcctctggac gcggcgaccg gcgggaatga gatgcgaaac gggttacgaa
     1441 cgcctgagct tcgtttttgc cgccgacgga agcttgcggg aaagaaagtc gatagacaat
     1501 ttcaccattc cgtgtaaaag aaatcagaac tgaaaacgta tctaccgttt attgacgccg
     1561 attgcccgcc ggaaaacgtt ttccggcggg caatccgttc aaattccatt cgcgatttac
     1621 tatttgcgat tgacgaataa ttattttttc ggtctttcgt tcgcttttaa aactcgtttt
     1681 cgcaaacgaa tcgatttcgg agtgacttcg acaagctcgt cgtcggcgat aaattcaagc
     1741 gcctgttcga gcgataaatc cttgaccgga acgagccgca tcgcttcgtc ggcggatgag
     1801 gcgcgcatat tcgtcaattt cttttcttta atcgcgttga cgtccaaatc gacggcgcgg
     1861 gcattttcgc cgacaatcat tccctcgtaa accttgattt gcggcttgat gaaaagcgag
     1921 ccgcgctcct gtaaattaaa cagcgcgtag gtcgtcgctt cgcccatccg gtcggagacc
     1981 agggaaccgg tcgagcgacc tttcatttcg ccctgccatt tgtcgaaacg cagaaacaga
     2041 gtatttaaaa gccccgtgcc tttcgtctcc gtcagaaatt cgccgcgaaa accgattaac
     2101 ccgcgcgacg gaacttcgta ttccagccgg acgcgccccg tgccgttgtt aatcatcttg
     2161 gacatctgac cttttcttct gcctaaagct tcggtcacga cgccgataaa ttcttccggc
     2221 acgtcaatca cgacctgctc gatcggttcc tgcaattcgc cggttttttc atgtctgcgc
     2281 gtgatgactt ccggcttcga tacctgcaat tcgaagcctt cgcggcgcat catttcgatg
     2341 agaatcgaaa gctgcaattc gccgcgtccc gatactctga agctgtcggg cgaatccgtt
     2401 tcggcgactc gcaaagccac gttgccgagc aattcctttt cgagccgctc tttgatttgg
     2461 cgcgaagtga cgaattttcc ttccgtgccc gaaaacggcg aagtattgac gccgaaaatc
     2521 atcgcaatcg tcggttcgtc gacattaatc agcggaagcg gtttcggatt gtcgggcagc
     2581 gtaatcgttt cgccgatgtt gatgtcgtca aatccggcga gcgcgacgat ttcgccgacg
     2641 cccgccgatt cgaccgtttt gcggtcgagg ctttcaaaaa cgtatagctc cttgacgcgc
     2701 gttttctgcg tcgagccgtc gcgcttggcg accagcactt cctgattttt ggcaatctcg
     2761 cccgaataaa ttctgccgat ggcgagtctg ccgacaaacg ggttgtagtc gatgttggcg
     2821 accagcagtt gcagcgtgtc ggtgcgcaat tcgtggggcg cgggaaccgt ttcgataatt
     2881 tgttcgaaaa gcggtttgag acttttcgat tcgtccgcca gagctttttt cgccacgccg
     2941 tcgcgcgaga tggaatacaa aatcggaaat tcaatctgcc ggtcgtccgc gccgaggtcg
     3001 ataaaaaggt cgtaaatttc atcgacgact tcttcggggc gcgcgtcgtg acggtcgatt
     3061 ttattgacta aagcgatggc tggaagtttt agttcgagag cttttctgag gacgaaacgc
     3121 gtctgcggca gacagccttc cgccgcgtcg acgagcagga cgatgccgtc gaccattttc
     3181 aaaacgcgct cgacttcgcc gccgaaatcg gcgtgaccgg gcgtatcgac gatgttgatt
     3241 ttgtaatcgc cgtagcgaac cgacgtgttt ttcgccataa tcgtgatgcc gcgctcgcgc
     3301 tccaaatcca tcgaatccat cacgcggtca acgaccgttt cgttgtcgcg gtaagtgccg
     3361 gattgctgga gcatcgcatc gaccagagtg gttttgccgt ggtcgacgtg cgcgatgatg
     3421 gcgatgttgc gtattttttc tttagtaatc atagacgtaa atttgccgtt gcgggcaaac
     3481 gaaaataatg actgattaga ggcgaaaagg caaacgccgg taaatgcaaa acgcaaaatg
     3541 cagagtgcga aatgaaaact cgatttaaat tccgcactct taattctgga cggttttcct
     3601 gattaaaaaa gaccggacga aatcgatttc atccggtctc agccttcatt ctaaacgggt
     3661 tagaatttaa ggagcaatgt tcataccgat gggctggtcg ccgttctgac cgaacttaac
     3721 ggcgacaaaa tcattgtttt cgctgcgcag gacataccag gtgccgtccg tcggacgaaa
     3781 tacggcgata tcggtttttc tgtccgaatc gaatcttccg gcaaccggaa tgtcgccgct
     3841 cagcccgaat cgaataatcc tgtaatctcc gtcgatgctg cgcaggatat accagtttcc
     3901 gtccgagggt cggaacacgg caatatcgtt gacgccgtcg ccgtcaaaat cgccgctctg
     3961 cggaatgtcg ccgccggcgc cccaacggaa agctcccagc gcgccgtccg agctgcgatt
     4021 gatataccag gttccgtttg acggtcggaa aacagcgatg tcattttgac cgtcgccgtc
     4081 atagtcgccg acgaccggaa tatcaccgtt taatccccag cgaattgcgc tgtacgtacc
     4141 gtttgaactg tatctgatat accaggtgcc gtccgacggg cggaaaacga ccaaatcgct
     4201 gcggtcgtcg ccgtcgaaat cagccgccac cggaatgtcg ccgctagagc cccattgctc
     4261 ggcagaaaaa ccgccgtcgg aactgcgctg gatataccag tagccggaac cgccgctcgc
     4321 gcgaaatacc gacaagtcgc cgatgccgtc gccgtcgtaa tctcccgaaa gcgctttatc
     4381 gccggcgagc ccgaaacgct gcgcgcggaa gaaaccgtcc gagctgttga gagaatacca
     4441 gttgccgtcc gacgggcgga aaaccgataa atcaaaaagt ccgtcgcctt caaaatcgcc
     4501 gccgatgtga acggcgcgca gctgtccgcg aatttctccg tttggattgt tctggctgcc
     4561 gatgacgata taacacaagc cggtgcgaag ctgcgccgcc tgctcggctg taacggcaaa
     4621 agtctgaacc ggtaaaaagc cgcttcttcc gccgaccgtt ccgagattga aaactgccgg
     4681 agcgttttgt cccggcagcg ccggacaatt tatgctcgcc gtcgtctgat tgctcgacaa
     4741 gccgaaaaat ccggcggaaa cctgaagctg tgttcccgct tcatttagaa gcgcgaaggc
     4801 gaaaccgcga gcttgcgtgt taacctgcgg aacgacctga ttgcctcgca atttagctct
     4861 gaataaacgc gactgattcg gcgacggaga cggtgtcggc gaaacggtcg gacttgccgt
     4921 cggcgaaacg gtgggcgaaa ccgtcggcga tgctgtaggc gaaacagtcg gcgaaacagt
     4981 cggcgaaaca gtcggtgaaa ccgtcgggct gaccgtcgga ctaaccgacg gcgacgggct
     5041 cggcgacgga tttgcgcaga aaaacgtgcc ggaaagaacc gtcgtgctgc cgtttctgat
     5101 tgtgataacc gtgcccggag tgatgttcat ttgcgtatcg ataaacacct gcccgtttcc
     5161 gttatctcgc agctgtatgt cgccgacgtc cactccgccg acgaaaacat ccagcgtcgt
     5221 gttgtccggc agatttagaa agcgaacaaa aactttcagc tggttattgt tcgaatctct
     5281 gaattgggcg aaaccgcggg gcgtttgccc gttgacgggc gcgccggtca gcgtcgcaaa
     5341 caatggtccg tcggcgcacg cggtcggaga gccggtcggc gaaaccgtcg gcgtcggcga
     5401 acccgctccc gtaaacgtgc cggaaagtat attggtcgcg ccttgtctga ccgttatcac
     5461 gtcgccgctt tgaataaccg gcgtgttctg ctgatggtcg cttctcagct ccaattcgcc
     5521 cgtgcgcgac gaattgacgg ttatctgacc gaccagattg ttgtttatgt aaacgtctaa
     5581 aattgtgcct gcgggcaaat taaccgaaaa aatttccagt tcgaatttgc gcagattgtt
     5641 tgattcgagt ttgtactcgg cgatgccgaa gggagagagg ttgttaatcg gcgtgccgct
     5701 cagcgatgcc tgtagtttta gttgcggcgg attcggcgga ttgtttgccg aagccgtaat
     5761 gaagctgatt aacggactac taatccaagc caatacagcc agtgtggtga acacagacag
     5821 ccagcgtctg ctgaagagtt ttttttcttt catttcctac cctctttttc gtttgaaatg
     5881 ggaacccaaa atacaaaccg attttcagac acaaactgcg gttctaaaaa tcggcggtgc
     5941 aattaactta tgtaacaagc ctacttttta aaaattttaa tgcaaggttt ttttgtgtta
     6001 aacgaaaaga aatcttaagg aatttaaaat tcgcggattt tatagatttt tataataaaa
     6061 aagaccggac gaaattgctt ccgtccggtc tttacgcttc ggtaaaagtt tattttaatt
     6121 agcgaacgtc ttctttttcg acaatcagat tgtttctcac ctgaaagacg ttcgagacgc
     6181 cgtttgccag aatgttcatc aggtcgtaat cgccgcgatt gcgaacgtag ccttcgagct
     6241 cgacgcgccc gccctgaacg ataattctca ccgacgggtt cggctcccac aggtatcgat
     6301 acaaaccttt atcggaaaag gagcgcagga tttgacgacg aatcgagtta tcaaatccgg
     6361 aaggcggcaa aacttcgata ttgttgatga ccc
//
LOCUS       DASWNE010000242         6759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_572923, whole genome shotgun sequence.
ACCESSION   DASWNE010000242 DASWNE010000000
VERSION     DASWNE010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6759
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572923"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VGB68_04935"
     CDS             complement(<1..215)
                     /locus_tag="VGB68_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048607.1"
                     /translation="MEEASRVLPLAVATLVCFLAAVTLGYGSWYFFGSHRWEKNTQDL
                     GYTVTNIEPVNTAPKEDIAETQPSPAS"
     gene            complement(404..916)
                     /gene="fliJ"
                     /locus_tag="VGB68_04940"
     CDS             complement(404..916)
                     /gene="fliJ"
                     /locus_tag="VGB68_04940"
                     /inference="COORDINATES: protein motif:HMM:NF028099.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HEY0048608.1"
                     /translation="MAFELTGMKSKKYRLETVLEIRGRVKDEAARQVAVRLEQLARAE
                     AELKRRLKELENCRERRRKAQNAMFTELSAGTQAHNITAHKDFLGQLKETENELEAAV
                     EQQKQTVAAAEREVAQAREKLIEAAKDLKAIEVHKSNWKITERTAETRREQKISDEIG
                     AILHGRRGQS"
     gene            1197..1934
                     /locus_tag="VGB68_04945"
     CDS             1197..1934
                     /locus_tag="VGB68_04945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0048609.1"
                     /translation="MAERIPEQQQFSAAGQSLVDRDALVERYRPFVFSVVKQVLNTLP
                     VKVDFEELVSYGMVGLLEAADRYDPRRKASFITFSHYRIKGAIFDGLRQMGILTRTPN
                     DAWVRRESVVNDLLQTVADDESENPSANSVDEEIQGVSRWIDSLIPAYLLSLSDENAP
                     DVVDSHELPDKAVEFQNTIAVLRNAVGELPPKEKNLIEALYFKQITTTELAKQMGVNK
                     SWVSRLHAKAVERLRQRLINIGFLPSS"
     gene            2316..3353
                     /locus_tag="VGB68_04950"
     CDS             2316..3353
                     /locus_tag="VGB68_04950"
                     /inference="COORDINATES: protein motif:HMM:NF019123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1521 domain-containing protein"
                     /protein_id="HEY0048610.1"
                     /translation="MGDVNFNIFNFNAGGGEPNFFQNLNPVAFQDINGILSDIFEDFA
                     KLFDAFGGNQFNSGDQQGIWAGGGGCVPPSPDDSTHPSGSLKTDAGVITTPGGYKIEA
                     TGQFEWTITGPDGKSTRVWGDPHVAEGDGGKWDFKRNSTFVLGDGTRINVSTAPWGNG
                     GMTVTSGLEIISGNERVTVSGIDKGKGVVGEVTQDGYAHSNTFGGNDVFVMGSETDDW
                     SYQGKEIIGSENGGESFKLGGDLAAGNRRPFTGYDSVDWMQLVRDLGGDMSNNWNDFW
                     RPNEMGSNPYYDGVSNRNDVRAGERYDRGRHRNQMAQAFRLLGQMFNVLSRLANLSDR
                     MYAQRNQSIYA"
     gene            3429..3935
                     /locus_tag="VGB68_04955"
     CDS             3429..3935
                     /locus_tag="VGB68_04955"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0048611.1"
                     /translation="MKENRDNKKSVTAHRSDQAREKLATGQVTLAEYVGLSREQMYKI
                     ARQAYQLMNRGKLEEARQIYSGLVAADPYDSVFHCHLGAVLFRLGEHEKAFEEYDAAL
                     RFNIANVDALAGRGELYIMRGEVKKGVEDLRSALEKDPQGERPTTQRARALLLSLREA
                     ARKQTTIN"
     gene            3953..6145
                     /locus_tag="VGB68_04960"
     CDS             3953..6145
                     /locus_tag="VGB68_04960"
                     /inference="COORDINATES: protein motif:HMM:NF037816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase A-like domain-containing protein"
                     /protein_id="HEY0048612.1"
                     /translation="MAIPPVKTSANLDLSASSGADKSVKAHAGINAPVGSQNQNSEAF
                     QNVNQQLGINNSQGNQNNQAAHGNSEGNNGNGNSAHASMQGSVQVSQRGNGNHNGHHQ
                     RGSSDAPRSNGNQGNQGNNGNHGVHHANHANHGSQAAPQSNQNEVGDGGRGNGNGNGY
                     AYGRAEGRGNDNGNGNGGNNKGGNPLNININFSANAQTSGNNPTGNNNQTSGNVQINV
                     NNQTSGNNRTGSNNQTTAQGQINNNSTQGNGNGRGNAYGHVNRSSQGHANHSTQGQIN
                     YSTTQNARQQQSTSLDLNLSLNLNRNGDNGNHNGIVRSVLNQVLRQNDIYLNQNVIRH
                     FVNENIGKPANGNGNHYGSRQNVSIPPEITQIVRTIRTQITSVLNNPSIKFNGNVRIN
                     IQHSPELSNLIHTSKNVLLQNFGAKPFINLNLNARMHIAAELILRYLSKSQANVLLNH
                     SAKQILNGLLLARGFAITADKFYPRHDIFSAKKFPLPYNVPPVVIRNLGNAVKSLIVE
                     ARLAQTPKDLEVAVQKFLRHPSGKNNPDALLSAVKLAGQFGVRGNAEGEAALPEKIAE
                     LVKFLILTGDKALQSVADAFDAAEAELSRGQNTDNSAGAESTELERNYETIRSGDNSA
                     EAEGALRRQLEFNPFFQNDRAASAFENPSEASRAQEEFSNRFHNEIEQWLQSGNHRFV
                     KDIELEKPIGIVVERGTSGFFTADKARIVLVRDGSVQGWHFLKSFLVK"
     gene            6151..6504
                     /locus_tag="VGB68_04965"
     CDS             6151..6504
                     /locus_tag="VGB68_04965"
                     /inference="COORDINATES: protein motif:HMM:NF036906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contact-dependent growth inhibition system
                     immunity protein"
                     /protein_id="HEY0048613.1"
                     /translation="MDNYSFRFPDLYQFFGGYFYQGWTTGYKWEGTSANFAAVVRHFK
                     AVNPPQTVARVQTQLQELLALELSEENLNDALTALGNNFYPPGENLNNREWLESIAEI
                     LRETSTKPRVLREIQ"
     gene            complement(6512..>6759)
                     /locus_tag="VGB68_04970"
     CDS             complement(6512..>6759)
                     /locus_tag="VGB68_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048614.1"
                     /translation="RQRARFASQPHGTVFTGRRRFDFSPVIKQARAQHLWRVSLYNRF
                     LPDKFLEKSPGDLCVEAFRSLRPILLLNAKEREEVFL"
BASE COUNT         1750 a         1733 c         1751 g         1525 t
ORIGIN      
        1 tggctcgccg gcgacggctg cgtttcggca atatcctctt tcggcgcggt gtttacaggc
       61 tcgatattcg tgacggtata tcctaaatcc tgcgtgtttt tctcccatcg atgcgaaccg
      121 aagaaatacc aggagccgta gccgagcgtg acggcggcga gaaaacacac aagcgtggcg
      181 acggcaagcg gcaaaacccg cgatgcttct tccaccgttt ttgcggaggc ggtcaagtgt
      241 atggatggct gcgacttctc cggcataaag ctttactttc agactttttt acaaatttta
      301 ccagcgccaa gtcacgattc gattaatcga cggttaaaaa tttttgttag cttcgattat
      361 aagctttccg ttcgcgcggc ggcaaccttg tttttcgtac ggctcaggat tgcccgcgcc
      421 gcccgtgcag aatcgcgccg atttcgtcgc tgattttctg ctcgcggcgc gtttccgccg
      481 tgcgctcggt aattttccag ttcgatttat gcacctcgat agcttttaaa tccttcgcgg
      541 cttcgattaa tttttcccgc gcctgcgcga cttccctctc ggcggcggcg acggtttgtt
      601 tctgctgctc gaccgccgct tccaattcgt tttcggtttc tttcaattgt cctaaaaaat
      661 ccttgtgcgc cgtaatgttg tgtgcctgcg ttccggcgct gagctcggta aacatcgcgt
      721 tttgcgcttt gcgccttcgc tcgcggcagt tttcgagctc tttcaggcgt cgtttcagct
      781 cggcttcggc gcgggctaat tgttcgagcc gcacggcgac ctgccgcgcc gcttcgtctt
      841 tcacccgccc gcgaatttcc agaaccgttt ccagacgata ttttttcgat ttcatcccgg
      901 ttaattcaaa tgccaatata taaagacgcc taaagcgcgc ggaatttctt taattttata
      961 aagcaaaccg caaattgccc gccgagacgc gtttggtaaa cgcgtttgat tataaataat
     1021 ccggctggga tgcaatattt ttgctcatag ttattaattg ttaattacca gcctcgcccg
     1081 cgtcggctga atttattatt ttattcagct ttccgcgtga caaaaattag ctttgctttc
     1141 tacgttcaaa taataataac attggtgttg gaaatttagt cgagtaaaat attcaagtgg
     1201 ctgaacgaat cccggaacag caacaatttt ccgccgccgg acaatctctg gttgaccgcg
     1261 acgcgctggt cgagcgctat cgcccgtttg tcttttcggt cgtcaagcag gttctgaaca
     1321 cgctgcccgt caaggtcgat ttcgaagagc tggtcagcta cggaatggtc ggcttgctcg
     1381 aagccgccga tcgttacgac ccgcgccgca aagcttcgtt tataaccttt tcgcattatc
     1441 gcatcaaagg cgcgattttc gacggcttgc gccagatggg aattttgacg cgcacgccga
     1501 acgacgcctg ggtgcgccgc gaatcggtcg tcaacgattt gctgcaaacc gtcgccgacg
     1561 acgaatcgga aaatccttcg gcaaactcgg tcgacgaaga aattcagggc gtcagccgct
     1621 ggattgattc gctgattcca gcttatctgc tttccctgag cgacgaaaac gcgccggatg
     1681 tcgtcgattc gcacgagctg cccgataaag ccgtcgagtt tcaaaacacc atcgccgttt
     1741 tgcgaaacgc cgtcggcgag ctgccgccga aagaaaaaaa tctcatcgaa gcgctttatt
     1801 tcaaacaaat cacgacgacc gagctcgcca aacaaatggg cgtcaataaa tcgtgggtct
     1861 cgcgcctgca cgccaaggcg gtcgagcgcc tgcgccagcg gcttatcaac atcggctttc
     1921 tgccgtcttc ataaaagaat aaaagaaaat taaccacaga tatacacaaa taaacgcgga
     1981 ttattaacag gatgagcagg atagccagga taaattaaaa ctaataacgt ctaagtttct
     2041 ttttatcctg taaatcctgt ctatcctgtt tgaatccctg tttatccagt gttcatctgt
     2101 ggtaaatttt ctttctcttt tctctctctg tgttccgtgc ggctaaaaaa attgtccgaa
     2161 aatctcctca cgcataggaa acatttccga aaagctgccg ataattaaca cagaagcaaa
     2221 tcagtttggt aaatccggag ataaaatcag ctagctgaat gcgaccaaac tcaaacaagc
     2281 aaaacgattt ttgattggag aggagatttc aggcaatggg agacgtaaat ttcaatattt
     2341 ttaattttaa tgcgggcggc ggcgagccaa acttttttca gaatttaaat ccggtcgcct
     2401 ttcaggatat taacggaatt ttatcggata tttttgaaga tttcgccaaa cttttcgacg
     2461 cattcggcgg caatcagttt aattccggcg accagcaggg catctgggcg ggcggcggcg
     2521 gatgcgtacc gccgtcgccg gacgacagca cgcatccgtc gggcagcttg aaaaccgacg
     2581 cgggcgtgat aaccacgccg ggcggctaca aaatcgaagc gaccggacaa tttgaatgga
     2641 cgattaccgg accggacggc aaatcgacgc gcgtctgggg cgacccgcac gttgccgaag
     2701 gcgacggcgg caaatgggat ttcaaacgca actcgacctt tgttctcggc gacggaacgc
     2761 gcattaacgt ttcgaccgcg ccctggggaa acggcggaat gaccgtcact tccggtctgg
     2821 aaattatcag cggcaacgaa cgcgtgaccg tttccggcat cgacaaaggc aaaggcgtcg
     2881 tcggtgaagt gacgcaggac ggctacgccc acagtaacac tttcggcggc aacgacgttt
     2941 tcgtgatggg cagcgaaacc gatgattggt cgtatcaggg aaaagaaatt atcggcagcg
     3001 aaaacggcgg cgagtctttc aagctcggag gcgatttggc ggctggcaat agacgcccgt
     3061 ttaccggtta cgattcggtc gactggatgc agctggtgcg cgatttgggc ggcgatatgt
     3121 ctaacaattg gaacgatttc tggcgaccga atgaaatggg gtcgaacccg tactacgacg
     3181 gcgtgtcaaa tcgaaacgat gttcgcgcgg gcgaacgcta cgaccgcggg cgtcaccgca
     3241 atcaaatggc gcaggcgttt cgtcttttgg gtcagatgtt caacgtgctt tcgcggctgg
     3301 cgaatttgag cgaccggatg tatgctcagc gaaatcagtc aatttacgcg taaggcgttt
     3361 aaaattcaaa gaccggtgcg aggcgtgttt atcggaaaat cacgcttcgc acgatttatc
     3421 ttttagagat gaaagaaaac cgcgacaata aaaaatctgt gacggcgcat cgctccgacc
     3481 aggcgcggga aaaactggcg accgggcaag tgacgctcgc cgaatacgtc ggcttgagcc
     3541 gcgagcagat gtataaaatc gcgcggcaag cctaccaatt gatgaatcgc ggcaagctcg
     3601 aagaagcgcg gcagatttat tccggcttgg tcgcggctga cccgtatgac agcgtttttc
     3661 attgtcatct gggcgcggtt ttgtttcggc tgggcgagca cgaaaaagcg tttgaagaat
     3721 acgacgcggc tttgcgcttc aacatcgcca acgtcgatgc gcttgccggt cgcggcgagc
     3781 tttacataat gcgcggcgag gttaaaaaag gcgtcgaaga tttgcgcagc gcgctggaaa
     3841 aagacccgca gggcgagcgc ccgacgacgc aacgcgcccg cgcgcttttg ctttcgctgc
     3901 gcgaagcggc gcgcaaacaa acgacgataa attaaaatct ttctgaaaaa ctatggctat
     3961 accgccggtc aaaacttcag ctaatctcga cctttccgct tcgagcggag cggataagtc
     4021 ggtcaaagct cacgccggca tcaacgcgcc ggtcggctcg cagaaccaaa attcggaagc
     4081 gtttcaaaac gtcaatcaac agctcggcat aaataattcg cagggcaatc agaacaatca
     4141 ggcggcgcac ggaaattcgg aaggaaataa cggaaacggc aattcggcgc acgcttcgat
     4201 gcagggcagc gtgcaggttt cgcaacgcgg aaacggcaat cataacgggc atcatcaaag
     4261 aggttcttca gacgcgccgc gcagtaacgg aaatcaagga aatcagggca ataacggaaa
     4321 tcacggcgtg catcacgcca atcacgccaa tcacggcagc caagccgcgc cgcaatccaa
     4381 tcaaaatgaa gtcggcgacg gcggcagagg aaacggcaac ggaaacggtt acgcctacgg
     4441 tcgcgccgaa ggtcgcggaa acgataacgg caacggaaat ggcggaaata acaaaggcgg
     4501 aaatccgtta aatattaata ttaatttttc ggcaaacgcg cagacgagcg gaaacaaccc
     4561 gacaggcaac aacaatcaga caagcggaaa cgttcagatt aacgtcaata atcaaacgag
     4621 cggaaacaac cgtaccggca gcaataatca aacgacagcg caaggtcaga taaataataa
     4681 ttcgacgcaa ggcaatggca acggtcgcgg caacgcgtac ggacacgtaa accgttcatc
     4741 gcaaggtcac gcaaatcatt cgacgcaagg tcagataaat tactccacga cgcagaatgc
     4801 gcggcagcaa caatcgacga gtctggattt aaatttgtcg ctgaatttaa acagaaacgg
     4861 cgataacggc aatcacaacg gaattgtccg cagcgtattg aatcaggtct tgcgacagaa
     4921 cgatatttat ctgaaccaga acgtgattcg gcattttgtg aacgagaata tcggcaagcc
     4981 cgcaaacggt aacggaaatc attacggaag ccggcaaaac gtttcgattc cgccggaaat
     5041 aacgcaaatc gttcgcacca tccgcacgca gattacaagc gttttgaata atccgtcgat
     5101 aaagtttaac ggcaacgtcc gcatcaatat tcaacactcg cccgagcttt cgaatttgat
     5161 tcatacaagc aaaaacgtac tgctgcaaaa tttcggcgcg aagcctttta taaacttaaa
     5221 tctgaatgcc cggatgcata ttgccgccga gctgattctg cgctaccttt cgaaatcgca
     5281 ggcgaacgtt ttgctcaatc attcggcgaa acagatttta aacgggcttc tgctggcgcg
     5341 cggatttgcg attacggctg acaagttcta tccgcgccac gacatttttt cggcgaagaa
     5401 attcccgctt ccatacaacg ttccgcccgt cgtcatccga aatctgggaa acgccgtcaa
     5461 atccctgatt gtcgaagcgc ggctcgctca aacgccgaag gatttggaag tcgccgtgca
     5521 gaaatttctg cgccatccga gcggaaaaaa caatccggac gctttattga gcgcggttaa
     5581 attagccggg caattcggcg tcagaggaaa cgccgaaggc gaagccgctc tgccggaaaa
     5641 aatcgccgag ctggtgaaat tcctgatttt aaccggagac aaagctttac aatcggtcgc
     5701 cgatgctttc gacgcggcgg aagccgagct gtcgagaggg cagaacaccg ataattccgc
     5761 cggcgccgaa agcactgagc ttgaacggaa ttacgaaaca atccggagcg gcgacaatag
     5821 cgcagaagcg gaaggcgctc tgcggcggca gttggaattt aatccgtttt tccagaacga
     5881 ccgcgccgct tccgcgtttg aaaatccgag cgaagccagc cgcgcacagg aagaattttc
     5941 caaccgtttt cacaacgaaa tcgagcaatg gctgcaaagc ggcaatcatc gcttcgtcaa
     6001 agacatcgag ctggaaaaac caatcggcat cgtcgtcgag cgcggaacga gcggattttt
     6061 tacggctgac aaggcgcgaa ttgtcctcgt gcgcgacggc tctgtgcagg gctggcactt
     6121 tctgaaatca tttctggtaa aataaccggc gtggacaatt attcgttcag gtttcccgat
     6181 ttgtatcagt ttttcggcgg atatttttat cagggctgga cgacgggcta taagtgggaa
     6241 ggcaccagcg cgaatttcgc ggcggtcgtc cgtcatttca aagccgtcaa cccgccgcag
     6301 acggtcgctc gcgttcaaac ccagttgcag gaacttctcg ccttagagct ttccgaagaa
     6361 aatttaaatg acgctttaac cgcgctcggc aataatttct atccgcccgg cgaaaatcta
     6421 aataatcgcg aatggctgga aagcatcgcg gaaatcctgc gggaaacttc gaccaaaccg
     6481 cgcgttttgc gcgaaattca atagaaataa tttataaaaa tacttcttcg cgctctttcg
     6541 cgtttaaaag caggattgga cgcaatgagc ggaaagcctc tacgcaaagg tcgccgggtg
     6601 atttttctaa aaatttatcg gggagaaatc gattgtacaa ggacacgcgc cataaatgct
     6661 gcgctctcgc ctgcttgatt accggggaga aatcaaatcg gcggcgtcca gtaaagactg
     6721 tgccgtgtgg ctgtgaggcg aatcgggcgc gctgccgat
//
LOCUS       DASWNE010000243         5754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_219099, whole genome shotgun sequence.
ACCESSION   DASWNE010000243 DASWNE010000000
VERSION     DASWNE010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5754
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219099"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="VGB68_04975"
     CDS             complement(<1..786)
                     /locus_tag="VGB68_04975"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEY0048615.1"
                     /translation="MTILVLGAGRMGYGAVYDLIHNSPEVESVTVADFDLGKAVEVAE
                     KVGGKVSATQIDASNYDAVAELMKPHDSAISCVNYWYNVELSRAAIETGTNFCDLGGN
                     NYIVDEQLALDTAAKNAGISIIPDCGLAPGMVSVLAMHGANRLDETEEIHIRVGGLPQ
                     NPQPPLDYQLVFSVEGLINEYIEKARVIRDGEIIEIESMTELESLAFENFPALEAFQT
                     SGGTSTLPETFAGKLKELDYKTIRYAGHCERFKTLIDLGLCSSQ"
     gene            complement(870..1238)
                     /locus_tag="VGB68_04980"
     CDS             complement(870..1238)
                     /locus_tag="VGB68_04980"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY0048616.1"
                     /translation="MEERRRGERYLISFPIRVEWKTAEGNEMIEEGLTENVGESGALV
                     HLPRKLPAVGSEVNLIVTENPSDQVAVTAQVLRLERNAAHPQAALKLTGETEDWEKKV
                     YQYAAKIIAAQKPDELDDWN"
     gene            1580..2104
                     /locus_tag="VGB68_04985"
     CDS             1580..2104
                     /locus_tag="VGB68_04985"
                     /inference="COORDINATES: protein motif:HMM:NF018437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HEY0048617.1"
                     /translation="MKNLARGSVFLFSLVALIVSFFYVQPQVEAVTTSISADLSNSQQ
                     VFLQQEQNKKSVTKATVTETENQTTNNTIEDKTANSASKNAGGFNATAYCLRGRTASG
                     GSVRRGIVAADPRVLPLGTRIYLNAGAYSGSYVVADTGGGVKGRKLDIWVPNCSEAMK
                     FGRRSVSVNILGRG"
     gene            complement(2190..2765)
                     /locus_tag="VGB68_04990"
     CDS             complement(2190..2765)
                     /locus_tag="VGB68_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048618.1"
                     /translation="MGIIYDLTREAIVLDAEVTIEGEGIKRTYPAGINLYSEYEFYVP
                     AGNYNITTKQNWLYAVKRASFYVSAGKTKIINLYPSQRFSAIVLSLDGDEVTYLTPPK
                     YAFLKIDNSASDAGIKFYDQKNGSTTIKYTSAQFTFDALTVLADEIIYNKKTKTFKFT
                     GDVLIDDNGRRTKAKKAEIRFKKGKTNLSFN"
     gene            complement(2936..3406)
                     /gene="msrA"
                     /locus_tag="VGB68_04995"
     CDS             complement(2936..3406)
                     /gene="msrA"
                     /locus_tag="VGB68_04995"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY0048619.1"
                     /translation="MKGVEDVVSGYSGGHTENPTYREVCSETTGHAEVTQIKFDPEVI
                     SFKEILQVFFSVHDPTTLNRQGNDIGSSYRSAIFYHDENQKRIAEEVIREVTEEGVYD
                     DPIVTEVTAFDKFYPAEDYHQEYFANNPNQPYCAAVVAPKVAKFRKKYVDRLKK"
     gene            complement(3532..4254)
                     /gene="mtnC"
                     /locus_tag="VGB68_05000"
     CDS             complement(3532..4254)
                     /gene="mtnC"
                     /locus_tag="VGB68_05000"
                     /EC_number="3.1.3.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR01691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acireductone synthase"
                     /protein_id="HEY0048620.1"
                     /translation="MTEAILLDIEGTTTPIDFVHRTLFPFALERIGEYLERSFDSIQI
                     ELAELRAEHARDFQSGLDAPPLDEATREKAIKSLTGYLRFLIGIDRKSTPLKSLQGKI
                     WQQGYESGELVSEVFDDVPAAFERWKNRGKTIAIYSSGSVLAQKLLFKYTNHGDLTAF
                     ISGYFDTRAGHKREAGSYRKIASTPSFPPVENFLFISDIEAELDAALEAGMQTLLVVR
                     AGNAPLAENPKHQTIHSFDEAP"
     gene            complement(4251..4481)
                     /locus_tag="VGB68_05005"
     CDS             complement(4251..4481)
                     /locus_tag="VGB68_05005"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0048621.1"
                     /translation="MIYALLPEQTGKGFAAEAASEIIEYAFSDLSFDYLDASCDLPNR
                     ASQKVAARLGMKIYKQEEINGRASVFFRLENK"
     gene            complement(4509..4766)
                     /locus_tag="VGB68_05010"
     CDS             complement(4509..4766)
                     /locus_tag="VGB68_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048622.1"
                     /translation="MSDVELELKTERLMLKPVARGDSEILERIITDRFVCRYLFDDRT
                     LDRNEIAELINLSRETFSDKKYGLWLIVLDKTGEIIGFTGL"
     gene            complement(4763..5284)
                     /locus_tag="VGB68_05015"
     CDS             complement(4763..5284)
                     /locus_tag="VGB68_05015"
                     /inference="COORDINATES: protein motif:HMM:NF015065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0048623.1"
                     /translation="MAVLNVPGKNLTFSEAQEIAGYLAKIGIDYERWETDSRISPDAA
                     PEEILAAYAGEIEKLKAAGGYVTADVIDIKPETPNLESMLERFRPEHWHDEDEVRFIV
                     KGRGCFHIAPPGADVVSITMEAGDLIRVPRGTKHWFDLCEDRTVRAIRLFQDISGWTP
                     HYTKSGLEKNYRV"
     gene            complement(5307..>5754)
                     /gene="mtnB"
                     /locus_tag="VGB68_05020"
     CDS             complement(5307..>5754)
                     /gene="mtnB"
                     /locus_tag="VGB68_05020"
                     /EC_number="4.2.1.109"
                     /inference="COORDINATES: protein motif:HMM:TIGR03328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylthioribulose 1-phosphate dehydratase"
                     /protein_id="HEY0048624.1"
                     /translation="ETQFLELDDNSDVLQGFGKPSAETLLHLTIYRMRNPGAILHTHS
                     VWGTILSDVFFENGAIEIEGYEMLKGLNGVRTHEHTEVLPIIENSQDYVALSHVAENV
                     LRENPDCHGIYLRRHGLYTWGKDVTEAKRHVEIFEFLFEVLGRQIK"
BASE COUNT         1426 a         1492 c         1287 g         1549 t
ORIGIN      
        1 ctggctcgaa cacaagccca aatcaatcag tgttttaaat cgttcgcaat gcccggcata
       61 acggattgtt ttataatcga gttccttgag tttgcctgca aaagtttccg gcaaagtcga
      121 agtgccgccg gaagtttgaa aggcttccag agcagggaaa ttttcaaaag cgaggctttc
      181 gagttcggtc attgattcaa tttctataat ttcgccgtcg cgaataacgc gagctttttc
      241 gatatattca tttatcaagc cttcgaccga aaaaacgagc tgataatcga gcggcggctg
      301 cggattctgc ggcaaaccgc cgacgcggat gtgaatttct tccgtctcgt ccagacgatt
      361 tgcgccgtgc atcgccagga cggaaaccat tccgggcgcc agcccgcagt cgggaataat
      421 gctgattccg gcgtttttcg ctgccgtgtc cagagccagc tgctcgtcaa cgatgtaatt
      481 gttgccgccc aaatcgcaaa aattcgtgcc ggtttcaatc gccgccctgg aaagctcgac
      541 gttataccag taattaacgc acgaaatcgc cgaatcgtgc ggcttcatca attcggcgac
      601 cgcatcgtaa ttcgaagcgt caatctgcgt cgccgaaact ttgccgccga ccttttccgc
      661 gacttcgacg gcttttccca aatcgaaatc ggcaaccgtg accgattcga cctccggcga
      721 attgtgaatc aaatcataaa ccgcgccgta tcccatccgc cccgcgccga gaaccagaat
      781 tgtcatattg tcagaaagat tattacttcg aaaggcgcaa tgatacagag aaatccgccc
      841 ggctgccaag cgccttctgc catccggttt cagttccagt cgtctaactc gtcgggtttt
      901 tgcgccgcga ttatcttcgc cgcatactgg taaacctttt tttcccaatc ctcggtctcg
      961 cccgtcaatt tcagcgccgc ctgcgggtgc gcggcgttgc gctcaagccg taagacctgc
     1021 gccgtcacgg cgacctggtc ggacggattt tccgtgacaa tcagattgac ctcgcttccg
     1081 accgccggca gcttgcgcgg cagatgcacc agcgcgccac tttcgccgac gttttccgtc
     1141 agcccttctt caatcatttc attgccctca gccgttttcc attccacgcg aatcgggaag
     1201 gaaatcaggt agcgttctcc gcggcggcgt tcttccataa ctggtaaatt ctccgttttt
     1261 caaactaaaa atggcttttg aaatatacga aattcattcg cttaagtcta ttaaaaagag
     1321 tagttttgct ccgatttttt cgccccgatt tcgtaagtga tagatgcgat tgcttttatg
     1381 ggttggacaa aattcattta tctgtgctac atttataaat gtcctaagca gacacttttc
     1441 gcggcaaccg ccgcaacaac ccttcagcct aagttttatg tatctcaagt cggctgttac
     1501 agatgtgttt tctcacgaaa acgctcttag cttttttggg atttgggctg aggaaaaata
     1561 gaaaaggagc aattgataaa tgaaaaatct agctagagga agcgtgttcc tcttcagctt
     1621 ggtcgcgttg attgtaagtt ttttttacgt tcaaccgcaa gtcgaagccg ttacaacttc
     1681 catctcagcg gatctgagta attcgcaaca agttttttta caacaagaac aaaacaaaaa
     1741 aagcgttacc aaagccacgg taaccgaaac cgaaaaccaa actacaaaca atacaatcga
     1801 agataaaacc gccaattccg cttccaaaaa tgccggcgga tttaatgcga ccgcatattg
     1861 cctgcgcggc agaaccgcca gcggcggaag cgtccgtcgg ggaatcgtag ccgccgaccc
     1921 gcgcgtttta ccgctcggaa cccgcattta tctaaatgcc ggcgcgtaca gcggaagcta
     1981 cgtcgtcgcc gatacgggcg gcggcgtcaa aggacgcaaa ttggacatct gggttccaaa
     2041 ctgctcggaa gcgatgaaat tcggtcgccg cagcgtctcg gtaaacattc tcggcagagg
     2101 ctgagcgaaa tttttacaat acttttaatt taataatgtg gaaatcgttt cgatacggga
     2161 aacggtttct tttttttctt aaaagccggt taattgaaag ataaattcgt ttttcctttt
     2221 ttaaatcgaa tttctgcctt tttcgctttt gtgcgccgac cgttgtcgtc gattaagaca
     2281 tcgcctgtaa acttaaatgt ttttgtcttt ttgttataga ttatctcatc agccagaacg
     2341 gttaaggcgt cgaaggtaaa ttgagcgctc gtatatttaa tggttgtaga accgtttttt
     2401 tggtcataaa atttaatacc cgcatccgaa gccgaattat ctatttttaa aaaagcatat
     2461 ttaggcggcg taaggtaagt aacctcgtcg ccgtccagcg acaaaacgat tgctgagaat
     2521 cgctgcgacg ggtataaatt aatgattttc gttttgcccg ccgagacata aaaactcgcg
     2581 cgctttaccg catacagcca attttgtttc gtcgttatat tataattacc ggctggcaca
     2641 taaaactcgt attccgaata taaatttatt cccgccggat acgttcgctt tattccttcg
     2701 ccttcgatgg taacttcggc gtccagaact atcgcttctc tggttaaatc gtaaatgatt
     2761 cccatgacct taccgttctc gaccggcttt acctgcgcgg tgacaatgat acagtcaaaa
     2821 acaatgacgg caagaaaaag gctaaacgtt tttatattca taagcgagaa tgaaaaatga
     2881 gaagcgaaaa tttttcgaca attttcgctt ccctttcacc tgttatttaa attatttact
     2941 ttttcagtct atcgacatac tttttgcgaa atttcgccac tttcggcgcg acgaccgccg
     3001 cgcagtacgg ctggttcgga ttgttcgcaa aatattcctg gtgataatct tccgccggat
     3061 aaaatttatc gaacgccgtt acttccgtca caatcgggtc gtcgtaaacg ccttcttcgg
     3121 tcacttcccg aatcacttct tcggcaattc gcttttgatt ttcgtcgtga taaaaaatcg
     3181 ccgagcgata ggaactcccg atgtcgttgc cctgccggtt taaagtcgtc gggtcgtgaa
     3241 ccgagaaaaa gacctgcaaa atttccttaa aggaaatcac ttccgggtcg aatttaatct
     3301 gcgtgacctc ggcgtgtccg gtcgtttccg agcaaacttc gcgataagtc ggattttccg
     3361 tatgcccgcc cgaataaccg gaaactacgt cttcgacgcc cttcaaatct tcttaaaccg
     3421 cttcgacgca ccagaagcag cctgctccga gcgtcgccgt ctgtaaatta ttattttcca
     3481 taaccctctg ataatgcctg aaataatttg tttattccat tctatcgaaa tttacggcgc
     3541 ttcatcaaaa ctgtgaatcg tttgatgttt cggattttcc gccaaaggtg cattacccgc
     3601 gcgaacaacc agcagagttt gcattccggc ttcaagcgcc gcgtccagct ccgcctcgat
     3661 gtccgaaata aacagaaagt tttccacagg tggaaaactc ggcgtggaag cgattttccg
     3721 ataactcccg gcttcgcgct tatgcccggc gcgagtatcg aaataaccgg aaataaaagc
     3781 ggttaaatca ccgtgattgg tgtatttgaa aagaagtttt tgagccagaa cgctgcccga
     3841 agaataaatc gcaatcgttt ttccacgatt tttccaacgc tcgaacgccg ccggaacatc
     3901 gtcgaaaact tcggaaacaa gctcgcccga ttcatagccc tgctgccaga ttttgccctg
     3961 taaagacttt aaaggcgtcg atttgcggtc aatcccgatt aaaaatcgca gatagccggt
     4021 caggctttta atcgcctttt cgcgcgtcgc ttcgtccagc ggaggcgcat ccaaaccgct
     4081 ttgaaaatcg cgcgcgtgtt ccgcccgaag ctcggcgagc tcgatttgaa tcgaatcgaa
     4141 gctccgttcc agatattcgc cgattctctc gagagcgaaa ggaaaaagcg tccggtgaac
     4201 aaaatcaatc ggcgtcgtcg tgccttcgat gtcgagtaga atcgcctcgg tcatttgttt
     4261 tccaatctaa agaaaacgct cgcgcgcccg ttaatttcct cttgtttgta aattttcata
     4321 ccgagccttg cggcgacttt ttgcgaagcc ctgttcggca aatcgcagct cgcgtccaga
     4381 taatcgaaag acaaatcgga aaaagcgtat tcgatgattt cgctcgcggc ttcggcggca
     4441 aagcctttcc ctgtctgttc cggcagcaaa gcgtaaatca attgcggctg gagttcgtca
     4501 aaaaaatgtc ataagcctgt aaagccgatt atttctccgg ttttatcaag aacaattaac
     4561 cagagtccgt attttttgtc gctgaaagtt tcgcggctga gattgataag ctccgcaatt
     4621 tcgtttcggt cgagagttcg gtcgtcaaat aaatatcggc acacaaatct gtcggtaatg
     4681 attctttcca gaatttccga atcgccgcgc gcgaccggtt tcagcatcaa tctttcggtc
     4741 tttaattcta attccacgtc gctcaaaccc gataattttt ctccagcccg cttttcgtgt
     4801 aatgcggcgt ccagcccgaa atgtcctgaa aaagacgaat cgccctgacg gttcggtctt
     4861 cgcacaaatc gaaccagtgt tttgtgccgc gcggcacgcg aatcaaatcg cccgcttcca
     4921 tcgtgattga aacgacgtcc gcgccgggcg gcgcgatatg aaaacagccg cgacctttga
     4981 cgataaaacg cacctcgtcc tcgtcgtgcc agtgttcggg gcgaaaacgt tcgagcatcg
     5041 attccagatt cggcgtctcc ggcttgatgt caatcacgtc cgccgtcaca taaccgccgg
     5101 cggcttttaa tttttcgatt tcgcctgcat acgccgcgag aatttcttcc ggcgcggcgt
     5161 cgggcgaaat tctcgaatcg gtttcccaac gctcgtaatc gatgccgatt ttcgctaaat
     5221 aaccggcaat ttcctgcgct tcgctaaaag ttaaattttt gccgggaaca tttaaaactg
     5281 ccataaaaat ttatttaaaa cccaaattat ttaatttgcc ttcccagaac ttcaaacaga
     5341 aactcgaaaa tctcgacgtg ccttttcgct tccgtcacgt cttttcccca ggtgtaaagc
     5401 ccatgacggc gcaggtagat gccgtgacaa tcagggtttt cgcgaagcac gttttcggcg
     5461 acgtgcgaga gcgcgacgta atcctgcgag ttttcgataa tcggcaaaac ttccgtgtgc
     5521 tcgtgcgtcc ggacgccgtt caaacctttc agcatctcgt agccttcgat ttcaatcgcg
     5581 ccgttttcaa aaaaaacgtc cgacagaatc gtgccccaga ccgaatgcgt gtgcagaatc
     5641 gcgcccggat tgcgcattcg ataaatcgtc agatgcagaa gcgtttcggc ggaaggcttg
     5701 ccgaagccct gaaggacgtc gctgttgtcg tcgagttcga gaaactgcgt ttcg
//
LOCUS       DASWNE010000244         4032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_7840, whole genome shotgun sequence.
ACCESSION   DASWNE010000244 DASWNE010000000
VERSION     DASWNE010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7840"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="VGB68_05025"
     CDS             complement(<1..610)
                     /locus_tag="VGB68_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048625.1"
                     /translation="MAKKKFDTNPLDPEFPERLKEAETTALPKNNFKTTEFPPHSVTE
                     EETRRFNEAEFNAFSSPYDGAQVPANFQTAEINAQTEDISRKVAKVGLPENILTALPY
                     IPFYIGLISGLLILLFVPKGETKVRFHAAQGLAAHIGIFIVSAILGLFGNVTGMANIG
                     SWIFQIVTTVMLIIFAVKAWQGQPVHIESVDDLTEWLEEKIKP"
     gene            complement(1062..1853)
                     /locus_tag="VGB68_05030"
     CDS             complement(1062..1853)
                     /locus_tag="VGB68_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048626.1"
                     /translation="MEKESSKENQFENDWKLYQEFLKAWADGSLDESNPVCQMVTESS
                     LTIHKRYKYKEEVDSMRQICFLVLTNGQYKGKTSLKTYIWGIYIWAIKHYELRRRQKK
                     GKESLEIKDWIIERVTEEVIQRMSSEQHMKTLLASVPKFDKKLIETIAKNPVTTAKQI
                     THNTHSKQFVSPEQADLLHKINNIFPTEKRQRYNELYARFKSEDLGENEYEELLELSN
                     EFEILNANRLKYIGKLAKLRGQSLEQVIDSFEIPNSDNEKEIHFG"
     gene            complement(1920..3728)
                     /gene="aspS"
                     /locus_tag="VGB68_05035"
     CDS             complement(1920..3728)
                     /gene="aspS"
                     /locus_tag="VGB68_05035"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEY0048627.1"
                     /translation="MLDTLGNLERTHTCGELRESNVGEQVVLMGWVAKKRDFGIFTFI
                     DLRDRTGVTQAVVSEETAEKAHAKAKNLRGEFVVAVKGEVVSRTEGTHNAKLATGDIE
                     IKVGELLILNDAKTLPFELEKAGSQNLASEDLRLKYRYLDLRRPKLQQNIVMRAKTIA
                     RMREYMEGQGFIEIETPILLKSTPEGARDFVVPSRIHKGKFFALPQSPQILKQITMIA
                     AFDKYYQIARCFRDEDLRADRQPEFSQLDVEMSFTNQEIIYRLLEEMFGHVFKLIDVE
                     LPAEFPRMTYAEALRRYGSDKPDLRFEMELQDLSENLQGTDFAPFASVLENKGEIKAI
                     VVKGKADYSRKALDELQEFAKRYGAGALAWIKLGDETTSSLLKVLGEAKIAELASAAK
                     AEKGDLVLIVAGRKSIVAASLGALRLEAAKRENLIPKDVFKPLLVTEFPMFEHDEATD
                     TYAAAHHPFTSPMDEDLEMFKTAIEDESQHDLLGRVRAKAYDAVINGIECAGGSIRIH
                     QRDVQALNFKALGLSPEKARTRFGFFLEALEYGTPPHGGFAAGIERTLMALLQTDNIR
                     DLIAFPKTASAQDLMMDSPGEVDASQLNELGIKIVE"
     gene            complement(3757..>4032)
                     /locus_tag="VGB68_05040"
     CDS             complement(3757..>4032)
                     /locus_tag="VGB68_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048628.1"
                     /translation="RYETIDSILNNWANKRNLHLYKNYKDEEVRSFEIVNTQGDRYQI
                     WVDAPDEKGQIGIYVWDYKKRRKDFIAQEVELFEKLESAFSFTQLEI"
BASE COUNT          959 a          991 c          876 g         1206 t
ORIGIN      
        1 ttggtttgat tttttcttcc agccattcgg ttaaatcgtc gaccgattcg atatgcacgg
       61 gctgtccctg ccaggctttg acggcgaaaa ttatcagcat cacggtcgtc acgatttgaa
      121 aaatccagct tccgatattc gccatacccg tgacgtttcc gaacaagccg agaatcgcgc
      181 tgacgataaa aatcccgatg tgtgccgcca gcccctgcgc cgcgtgaaag cgcactttcg
      241 tttcgccttt cggaacgaaa agcagaatca gcaagccgga aatcaagccg atgtaaaaag
      301 gaatatacgg caaagcggtc aaaatatttt ccggcaagcc gaccttggcg accttgcgcg
      361 aaatgtcttc cgtttgcgca ttaatttccg ccgtctggaa atttgccgga acctgcgcgc
      421 cgtcgtatgg cgagctgaaa gcgttaaatt ccgcttcatt aaatcttcgg gtttcttcct
      481 cggtcaccga atgcggcgga aattcggttg tcttgaaatt gtttttcggc aaagcagtag
      541 tctcggcttc cttcaatctt tcgggaaatt ccgggtcgag cgggttggtg tcgaattttt
      601 tctttgccat gatgattaaa ttttacgaca tctctaccag tttagttcgg aaaattcatc
      661 gtttattcgg gcggatgatt accatattcg cgtaatatcc ttctttgatt gattaatcct
      721 tgccggttca aattcaaggc tttaatggtt acgcgcccga ttgaagtcaa tccgacgatt
      781 tcgccaaaat cgctcgtcca tgtaaaatgc tcgtgccaaa tatcaatacg cgggttaaaa
      841 agtctgacgg ttttatcgct actgcatcga aaaattccgt tttgttcgat ttgaataaat
      901 tgcagccgtg acaagctagt gcgagattac tcaaatcgga ttttccgcct ccggattcag
      961 gcagaatatg gtctatatca aacggactgt cggaaaatct ggagtttgaa cgacaatatt
     1021 cacaaaatcc tttggcttgt aatatcacct ctcgacggat tttagccgaa atggatttct
     1081 ttttcattat ccgaattagg tatctcaaac gaatctatta cttgttcgag agattgcccg
     1141 cgaagcttgg cgagcttacc gatatacttt aagcggttcg catttagaat ttcaaattca
     1201 ttgctcaatt ccaaaagttc ttcgtattca ttctctccta aatcttcgct tttgaatctt
     1261 gcgtaaagct cgttatatct ctgtcgcttt tcggtcggaa agatattatt tattttatga
     1321 agtaaatcag cctgctcagg cgaaacgaat tgttttgaat gagtattatg tgttatttgt
     1381 ttcgctgtcg ttacagggtt tttggcaata gtttcaatga gtttcttatc gaatttaggc
     1441 acactagcca gcaatgtttt catatgctgc tcggaagaca ttctctgaat tacttcctca
     1501 gtaactcgct caataatcca atccttgatt tcgagtgact ccttaccttt cttctgacgc
     1561 cgccgcaatt cataatgctt tattgcccaa atatagattc cccaaatata ggttttgagt
     1621 gaagtttttc ctttgtattg tccgtttgtt aacacaagaa aacaaatttg acgcatacta
     1681 tcgacctctt ctttatactt ataccttttg tgtatagtca gagaactttc tgtcaccatt
     1741 tgacagactg gattggattc atccaaactt ccatctgccc aagctttcaa aaattcctga
     1801 tagagtttcc agtcattctc aaattgattc tctttgcttg attctttttc catgctttac
     1861 ttctttcggc tgtcgccttt cgcacatata agtttctgtt agaatcaatt atcgcacatt
     1921 tattcgacga ttttaattcc cagttcgttc agctgcgaag cgtcgacctc gcccggcgaa
     1981 tccatcatca aatcctgcgc cgaagcggtt ttcggaaagg caatcaaatc tcgaatgttg
     2041 tcggtttgca gcagagccat caaggtgcgc tcgattccgg cggcaaaacc gccgtgcggc
     2101 ggcgttccgt attcgagggc ttcgaggaaa aaaccgaaac gtgtccgggc tttttccggc
     2161 gaaagtccga gagctttaaa gttcaaagcc tgcacgtcgc gctggtgaat acgaatcgag
     2221 ccgcccgcgc attcgatgcc gttgatgacg gcgtcatacg ctttggcgcg aactcgtccg
     2281 agcaaatcgt gctgcgattc gtcttcaatc gccgttttga acatctccaa atcttcgtcc
     2341 atcggcgatg tgaaaggatg atgcgccgcc gcgtaagtat cggtcgcttc gtcgtgttcg
     2401 aacatcggaa attccgtgac cagaagcggc ttgaaaacat ctttcggaat taagttttcg
     2461 cgcttcgccg cttccagacg caaagcgccg aggctggcgg caacgattga ttttctgccc
     2521 gccacgatta aaaccaaatc gcctttttcg gcttttgccg ccgaagccaa ttcggcgatt
     2581 ttcgcttcgc ccaggacttt cagcaaagat gaagtcgttt cgtcgccgag ctttatccag
     2641 gcgagcgcgc ccgcgccgta gcgcttggca aattcctgca attcgtccag agctttgcgc
     2701 gaataatcgg cttttccttt aacgacgatt gccttgattt cgcctttgtt ttctagcacg
     2761 gaggcaaagg gcgcgaaatc cgtgccctgc agattttcgg ataaatcctg caattccatt
     2821 tcaaaacgca aatccggttt gtcgctgccg taccggcgca gcgcttcggc gtaagtcatt
     2881 cgcggaaatt cggcgggcag ttcgacgtcg attaatttga aaacgtgacc gaacatttct
     2941 tcgagcaggc gataaattat ttcctgattg gtaaacgaca tttccacgtc cagttgcgaa
     3001 aactcgggct ggcggtcggc gcgcaaatct tcgtcgcgga aacagcgcgc gatttgataa
     3061 tacttgtcaa aagcggcaat catcgtgatt tgctttaaaa tctgcggcga ctgcggcagc
     3121 gcgaaaaatt ttcccttgtg gatgcgtgaa ggaacgacga aatcgcgcgc gccttcgggc
     3181 gtggatttca gcaggatagg cgtttcgatt tcgataaatc cctgcccttc catatattcg
     3241 cgcattctcg caatcgtttt ggcgcgcatc acgatatttt gctgcaattt cgggcggcgc
     3301 aaatccagat agcgatattt cagacgcaaa tcttccgacg ccagattttg cgagccggct
     3361 ttttccagct cgaacggcag agttttcgcg tcgttcaaaa tcaaaagctc gccgaccttg
     3421 atttcgatat cgccggtcgc cagcttcgcg ttgtgcgttc cttcggtgcg cgagacgact
     3481 tcgcctttga cggcgacgac gaattcgccg cgcagatttt tcgctttggc gtgcgctttt
     3541 tcggcggtct cttcgctgac gaccgcttgc gtcacgcccg tgcggtcgcg caaatcgata
     3601 aacgtgaaaa tgccgaagtc acgttttttc gccacccagc ccatcaaaac gacctgctcg
     3661 ccgacgttcg attcgcgcaa ttcgccgcag gtatgagttc tttccaaatt tcctaatgtg
     3721 tctaacataa atcgtaaata tagattaaaa aatcatttaa atttctaatt gtgtaaagga
     3781 aaacgcgctt tcaagctttt caaataattc tacttcctga gcaataaaat cttttcttct
     3841 ttttttataa tcccagacat agattccaat ctgtcccttt tcgtccggcg catctaccca
     3901 aatctgatat cggtcgcctt gcgtattaac tatttcaaag gaacgcactt cttcatcttt
     3961 ataattttta taaagatgaa gattgcgctt attcgcccag ttattcaaaa tactgtcaat
     4021 tgtttcgtat ct
//
LOCUS       DASWNE010000245        11283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_148352, whole genome shotgun sequence.
ACCESSION   DASWNE010000245 DASWNE010000000
VERSION     DASWNE010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11283
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148352"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1296)
                     /locus_tag="VGB68_05045"
     CDS             complement(<1..1296)
                     /locus_tag="VGB68_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048629.1"
                     /translation="MATPPSRRTQTLTIIAQDPSVKVKRKKRGTDEEEEVILTARVDL
                     PAEELAPGPRGYRVQVVDYDSTAGVLYPPLEYRVLESGLYNDPFIDEPDSTLLDDPLF
                     HAQNVYAIVMRTLARFEYALGRRVSWSFDGHQLQIAPHAFTNANAFYSKRDRALLFGY
                     FPKSNGNDGDPLTTVFTCLSHDIVVHETTHALVDGLRERYTDPSSPEQAGFHEGFADV
                     VALLSIFSLPKVVASIIDKRFQNNGHSATNDPEAIDIKTLTKESLRESLLLGLAEEFG
                     EEISGTRGSALRRSVKLEPSVEHIKSPEFEEPHKRGELLAAAVMNAFIEVWVKRLEGL
                     GNRPPQNRVTRTQQTPGGQPAAASAEILLQEITTFDRLNRREQMKKGEEELDYTYSYL
                     DRRRVVEEGASIADYLLTMVIRALDYSPPSDIEFCDFLSA"
     gene            1778..2464
                     /gene="cmk"
                     /locus_tag="VGB68_05050"
     CDS             1778..2464
                     /gene="cmk"
                     /locus_tag="VGB68_05050"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEY0048630.1"
                     /translation="MIIAIDGPSGAGKSTLGKMLAKKLNLLYLDTGAMYRAVGLVVLE
                     SKTAFENTARVAEIVANAKIELVGEPENLQVKLDGRDVSREIRTGAVGQAASIVSTIS
                     EVRKILVEHQRRLGETAPHGCVLDGRDIGTVVFPNADVKFFLTAKPEARARRRYEEDL
                     ARGLAASYEKTFEEISLRDKRDTSRADSPLTIAEDAIVIDTSELNLTEVFERMSEIVR
                     EIERTNYGSA"
     gene            complement(2619..2972)
                     /locus_tag="VGB68_05055"
     CDS             complement(2619..2972)
                     /locus_tag="VGB68_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048631.1"
                     /translation="MATAADYSIITDGWVLQKNQDTINFNVPSNIDTGSRAILGFMLH
                     VENLESLTMTLRLNGTSVWNWNYADGKRFQYFQEVITAGVVKAGNNVFKFESSTDDFH
                     LVEVSDVVVWWRAKI"
     gene            complement(3028..3192)
                     /locus_tag="VGB68_05060"
     CDS             complement(3028..3192)
                     /locus_tag="VGB68_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048632.1"
                     /translation="MKESGKVLRRAGAFTAFYRTSVSRVFQVFADEISKVIYSGGASL
                     TRQILTRYTS"
     gene            3421..3864
                     /locus_tag="VGB68_05065"
     CDS             3421..3864
                     /locus_tag="VGB68_05065"
                     /inference="COORDINATES: protein motif:HMM:NF013154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HEY0048633.1"
                     /translation="MTVQDVDLAWLAGIIDGEGCFCIFTNSRKDAMKPSISANLTITN
                     SNCLLLNRCKEILDALEIKYVYQDPKNGHQRGRRVMRIKIKNYSSLQLIIELTLPFFV
                     GKAEQAKLILEFVSLAGERGKLRYDERAKLMSKIKELNRHGHVIA"
     gene            4107..5132
                     /locus_tag="VGB68_05070"
     CDS             4107..5132
                     /locus_tag="VGB68_05070"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HEY0048634.1"
                     /translation="MDLISLSERGLYVEAGDFYIDPWKPVKQAVLTHAHADHTYRGSK
                     NYLVSKEGERLFRLRLWNEGNIETLAYGETRTIGGVKVSFHSAGHVLGSSQVRVEYKG
                     EIWVASGDYKLTADPTCAPFEHVKCHTFITEATFGLPIYRWTPPDILFADVNDWWRTN
                     AEKGKASVIFAYSLGKAQRIMKSVDASIGKIYTHGAVERLTGEYRESGVALPPTQYVG
                     AVENKKDFVGSLVVAPPSAQDTPWLRKFGNASTAFASGWMRIRGARRRRAVDRGFILS
                     DHADWDELLKAISATEAEKILVTHGSTEPLVRYLQNQGVDARPLKTGYAGESPEMGLA
                     GTEIDED"
     gene            5350..6960
                     /locus_tag="VGB68_05075"
     CDS             5350..6960
                     /locus_tag="VGB68_05075"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016453714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEY0048635.1"
                     /translation="MKNFTDLYLALDQTNKTNEKVEAMVKYFSEATAEDAAWAIYFLS
                     GRKPRQIVPTAKLRAWAAEIAGVEDWLFELSYDAVGDLGETIALLLPDNSATGSDLSL
                     SYWIEKRILPLRDLAEAEQHEAVAQAWNELDADQRFVFNKFITGSFRVGVSQLLVVRA
                     LAQVAGIEKEIIAHRLMGTWSPNADFYLNLIEAAAADADLSRPYPFHLAHQIDFPLED
                     LGAISDWQAEWKWDGIRAQLIRRAGESFLWSRGEDLVTERFPEITEASFNLPDGTVLD
                     GEILPWKTGAVLPFAQLQRRIGRKTIGKKLLEEVPVVLLCYDILEFQGEDIRPKPMRE
                     RRAILTEILGNLPGESKKSLLLEPVVEAKDWKKLAEIRAESRNRKVEGFMLKRLDSMY
                     RVGRPRGDWWKWKIDPLTVDAVLIYAQRGTGKRASLYTDYTFAVWRDGELVPFAKAYS
                     GLTDAEIRKVDRFVRNNTIESFGPVRSVTPQLVFELGFEGIQRSTRHKSGIAVRFPRI
                     LRWRDDKKIEDADTLETIHAMLDAEEGN"
     gene            7118..8686
                     /locus_tag="VGB68_05080"
     CDS             7118..8686
                     /locus_tag="VGB68_05080"
                     /inference="COORDINATES: protein motif:HMM:NF020048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048636.1"
                     /translation="MEKAQYPFLVLTTGERFPRLLVTRRPDLVGEKHTLYGAFLPETM
                     VRRLIDFIARVFRLHPCELDIDGSFDAPCPEYFLRRCLAPCVAEICDDEKYKSAVEAV
                     QLLLSRRADELLFKIDKEIGHLAENLEFERAAEARDIRRALSEIFTDSKWQIRAEDFT
                     DVLTATVENGEASVHLNTLRRGKAVGGKHSVWKLESENGDEGAALAEFIRDFYRFYLP
                     RRIYVSHDFPARKTLEKRLSEHFGKKAEIIAALPEKLPPTVFTAQKRAEILLANPVLQ
                     SGRDAEKIAAELKEIFNLENAPRRVECFDVAHLAGKEIVAARVVGRGGKLATDEDIVW
                     QFENLSETESLAESVRERLKLLPSRADLPDLILLDGGKPQMNAVKRVLREFDLENLPL
                     AAAVKPPRQHDKVSHFLTNPGEAKIDFNSRSPAMNFLQKLRDEAHALANRTHREVHSL
                     VQIFDRRIGSGAPKVQLLLVPTRYAARGGRAEDLSPIRSLNQSGEILFKAGKNQSSAG
                     EKHKRRSRFSRKAE"
     gene            8776..8851
                     /locus_tag="VGB68_05085"
     tRNA            8776..8851
                     /locus_tag="VGB68_05085"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8809..8811,aa:His,seq:gtg)
     gene            8989..>11283
                     /locus_tag="VGB68_05090"
     CDS             8989..>11283
                     /locus_tag="VGB68_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY0048637.1"
                     /translation="MKTVKFVFLFVLAFPLFSQIAFAQYNSRVYADGELLVKFKDGTV
                     SASAVSANSQFGARVVEEFPDLGWQRVKLPAGLPVETAVARYANLPDVEYVQPNYLYH
                     LQATPNDPQFSSAGMYGLTKISAPAAWDLTIGSANVVVANIDTGINYNHEDLAANMWT
                     NPGEINGNNLDDDGNGFIDDYYGYDFFFSDSNPQDENGHGTHTAGTIGAVGNNNVGVV
                     GVNWNVRLMAIKIYNSSGFGTTSAMLINAYNYVRLMKNRGVNIRVTNNSYGGCDEACG
                     YDQATKDALDALGNAGILNVFAAGNNGHNVETEAFYPASYTSPSILAVANSTSTDTRA
                     GSSNYGAISVDLAAPGTGILSTHTNVSGYANLTGTSMASPHAAGAAALLSARNPNLSP
                     ASLKATLMNTVDTLAAWNGVVKTGGRLNVDRALRNQTVCNFSLSAYEFRTSLDGGNGS
                     VNVAALTNCDYSIKSNASWIVINSGNTGSGNATINFTVQPFNVSDGMRTGTVTIADQT
                     FFVIQGQPFLPRNLLDFEGDGRTDYVAIQNDNGSMIWHINRSSSGYVTRNFGFFADDI
                     AVPNDFDADGVTDVAVWRNSNGTFYVLQSANNTFKGQQFGASGDNPNVTQDFDGDARA
                     DFAVTRKENGKLVWYMLNSITGFRAIQFGNENDRPIRGDYDGDNRADLAVYRPAADSP
                     ANTFIVLGSINNGLKTLTFGNSATDKVVPGDYDGDAKTDFAVWRTTDGTWYLIKSSTG
                     AFSGFQFGASGDLPAPGDYDGDGRT"
BASE COUNT         2749 a         2851 c         3043 g         2640 t
ORIGIN      
        1 cgcgctgaga aaatcgcaaa attcaatatc cgacggcggg ctgtaatcga gcgcccgaat
       61 taccatcgtc agcaggtaat cggcaatgct cgctccttct tcgacgacgc ggcggcggtc
      121 gagatagctg taggtatagt ccagttcttc ttcgcccttc ttcatctgct cccgtctgtt
      181 cagacggtcg aaagtcgtta tctcctgcag cagaatctcg gcggaagccg ccgccggctg
      241 accgcccgga gtttgctgcg tcctggtaac tcgattttgc ggcggtctgt ttccgagtcc
      301 ttccagacgc ttgacccata cttcaataaa agcgttcatc acggcggcgg caagcagttc
      361 tccacgcttg tgcggctctt caaactcagg gcttttgata tgttccacac ttggctccag
      421 cttaaccgaa cggcgcagag cgctgccccg cgtgcccgat atttcctctc cgaactcttc
      481 cgccaagccc agcagcagcg attcgcgcag ggattcttta gtgagggttt taatatcgat
      541 agcctccggg tcattggtcg ccgaatgccc gttattttgg aatcttttat caataatcga
      601 cgccacgact ttcggcagcg aaaaaatgga aagcagcgcg acgacatcgg caaagccctc
      661 gtgaaaaccg gcttgctccg gcgaagacgg gtctgtgtag cgctcgcgca aaccgtcgac
      721 cagagcgtgc gtggtttcgt gaaccactat gtcgtgcgaa agacacgtga aaacggtcgt
      781 cagcgggtcg ccgtcattgc cgtttgattt cggaaaataa ccgaacagca atgcccggtc
      841 tctttttgaa taaaaagcgt tggcattggt aaaagcgtgc ggagcgattt ggagctgatg
      901 accgtcgaag ctccagctga cgcgcctgcc cagagcgtat tcgaatcggg cgagcgtgcg
      961 catcacgatg gcgtagacgt tttgcgcgtg aaaaagcgga tcgtctaaca gcgtcgagtc
     1021 gggctcgtca ataaaagggt cgttgtagag cccgctttcg agcaccctgt attcgagcgg
     1081 cggataaaga acgccggcgg tcgaatcgta atcgacgacc tggacccgat aaccgcgcgg
     1141 accgggcgcc agctcttccg ccggcaaatc gacgcgggcg gtgagaatta cttcttcttc
     1201 ctcgtccgtt ccgcgttttt ttcgtttgac ctttacgctc gggtcctgtg cgataatagt
     1261 cagcgtctga gtgcgtctgg atggcggcgt agccataatt tttatctcct gaaataagta
     1321 ttccgggtaa ttcggaatta tttataaatc atcgggctgg tatctcagcc gttttttcct
     1381 gaagactgaa agcctgtaat tttttgatta aagaatattt ataagttccg gctcgattgc
     1441 gttcgcctcg ggttcaaatt tttaatgtca aacaaagaaa aaagccggac ttcaattcat
     1501 ctctgataaa aatcaatctg cccgcgcggg ctcgagctcg aaattaagga gatttccgga
     1561 cataattcca attaagtttt atttttaacc gcgccggaaa cccgccgaaa attgttttct
     1621 gcctggtatg attcggcggc gtgaaacgtt acaatttttt tgtgattttt ttcgatttat
     1681 ctcgaccggt gatttttcgg aacgcgcgaa gcggcaagct cccggagctt ttgattgatt
     1741 tagacagaat cgcggaaatc aaataaacta aaattcgatg ataatcgcca ttgacggacc
     1801 ttcgggagcg ggcaaatcca cgctcggcaa gatgttagcc aaaaaactga atctgcttta
     1861 tctcgacacg ggcgcgatgt accgcgccgt tggcttggtt gttttggaaa gcaaaacggc
     1921 ttttgaaaat acggcgcgcg tcgccgaaat cgtcgcaaat gccaaaatcg agcttgtcgg
     1981 cgagccggaa aatctacagg taaaactcga cgggcgcgac gtctccagag aaattcgcac
     2041 cggcgcggtc gggcaggcgg cttcgattgt ctcgacgatt tccgaagttc gcaaaattct
     2101 ggtcgagcat cagagaaggc tcggcgaaac cgcgccgcac ggctgcgttc tggacgggcg
     2161 cgacatcgga accgtcgttt tcccgaacgc ggacgtcaaa ttttttctga cggcaaaacc
     2221 ggaagccaga gccagaagac gctacgaaga agatttggcg cgcgggcttg cggcgagcta
     2281 cgaaaaaact tttgaagaaa tcagtttgcg cgacaaacgc gacacttccc gcgccgattc
     2341 gccgctgacg attgccgagg acgcgattgt aatcgacacc agcgagctga atctgacgga
     2401 agttttcgag cgaatgtcgg aaattgtgcg cgaaatcgaa aggacgaatt acggctcggc
     2461 ttagattggc ggcgggcaat ctggcttgaa ataaaaacaa accgcgccgc cggtgagcga
     2521 atgacggcaa agccgtttaa cggtcgccgc agccggacgt ttccggcatt ccgaaatccg
     2581 aagcgacagc ctcgccgcca ttcgtccctg acgccgattt agattttcgc ccgccaccag
     2641 acgaccacgt cggaaacttc tacgaggtga aaatcgtcgg tcgagctttc aaatttgaac
     2701 acgttattgc cggcttttac tacgccggcg gtaatcactt cctgaaaata ctgaaagcgc
     2761 ttgccgtcgg cgtaattcca gttccacacg ctcgtcccgt tcagccgaag agtcatagtc
     2821 aggctttcca gattttccac gtggagcata aagcccagaa ttgcccgcga gcccgtgtcg
     2881 atgttcgacg gcacattgaa attgatggtg tcctgatttt tctgtaaaac ccaaccgtcc
     2941 gtgataattg aatagtctgc tgccgtagcc atatttggtt tctccttttt tcgtttattt
     3001 acgcgctaat ttaagtacaa ctggaaatta agacgtatag cgcgttaaga tttgccgtgt
     3061 taaagacgcg ccgcccgaat aaatcacttt agagatttcg tctgcgaaaa cctgaaagac
     3121 acgcgaaacg ctcgttcgat aaaacgccgt aaacgcgccc gcgcgccgaa gaactttccc
     3181 ggactctttc aaactaatcc gccgccctta aacccgctta atttgacttg agattccaca
     3241 atcgctaaca tctaaacttg ctttttgata tgctcccgta gctcagttgg atagagcaac
     3301 agccttcaaa ctaataggag cctgtgcagg gaataagaaa cttgcacagt ggagagtgct
     3361 gtatcggtgg aactctaagg gaattttata aattcctatg acaataccga gcaaagtaat
     3421 atgacggtgc aagatgtcga tttagcatgg ttggctggaa taatagatgg cgaagggtgt
     3481 ttttgtattt ttaccaacag tcgtaaagat gcaatgaagc cttcaatatc ggctaacctt
     3541 acgattacta attcaaattg cttgttgctg aatcgctgta aagaaatact tgatgctctt
     3601 gaaatcaagt atgtttatca agacccgaaa aatggtcatc agcgaggacg cagagtaatg
     3661 agaattaaaa tcaagaatta ttcatctttg caactgatta tcgaattgac gcttcccttt
     3721 ttcgtcggaa aagccgaaca agccaaactt attttggaat ttgtcagttt agccggagaa
     3781 agaggaaaac ttcgatacga tgaacgggca aagttaatga gtaaaataaa ggaactaaat
     3841 cggcacggtc atgttattgc gtagagacta tacgcactca gactgaaatg tctaagatat
     3901 agtccagact ttcaacttgt tttctcacgg gaaaacaggg cttgggaaac caagtgaggt
     3961 aagctaagct gtgggtcgca cgttcaagtc gtgccggggg tactaaattt taaaaaggcg
     4021 gaaggttttc tgaaaggaag attttccgct tttttcgtct tcaaataaat tccctttcgc
     4081 ctttcccgat ttgttaaaat tgaaaaatgg atttaatcag tctcagcgag cgcgggctgt
     4141 atgtcgaggc gggcgatttt tatatcgacc cgtggaagcc ggtcaaacaa gccgttctga
     4201 cgcacgcaca cgccgaccac acttatcgcg gctcgaaaaa ttatctggtc tcaaaagaag
     4261 gcgaaaggct ttttcgcttg cgtctgtgga acgaaggcaa catcgaaacg cttgcctacg
     4321 gcgaaacgcg cacgattggc ggcgtcaaag tttcttttca ttcggcggga cacgttttgg
     4381 gctcgtcgca ggtgcgcgtc gaatacaaag gcgaaatatg ggtcgcttcg ggcgattaca
     4441 aattgaccgc agacccgacc tgcgcgccgt tcgagcacgt caagtgtcat acttttatca
     4501 ccgaagcgac tttcgggctg ccgatttatc gctggacgcc gcccgatatt ttgtttgcgg
     4561 acgtcaacga ctggtggcgc acgaacgccg aaaaaggcaa agcgtcggtg attttcgcgt
     4621 attcattggg caaagcgcag cgcattatga aaagcgtcga tgcttccatc ggaaaaatct
     4681 acacgcacgg cgcggtcgaa agattaaccg gggaataccg cgaatcgggc gtcgcgcttc
     4741 cgccgacgca atatgtcgga gcggtcgaaa ataaaaaaga ttttgtcggc tcgctcgtcg
     4801 tcgcgccgcc ttcggcgcag gacacgccgt ggctgcgcaa attcggaaac gcttcgacgg
     4861 ctttcgcttc cggctggatg cggattcgcg gcgcgcggcg gcggcgcgcc gtcgatagag
     4921 gttttatttt gtccgaccac gccgattggg acgagctttt aaaggcgatt tccgcaacgg
     4981 aagccgaaaa aattctggtc acgcacggct cgaccgaacc gctcgtccgg tatctgcaaa
     5041 atcaaggcgt cgacgcgcgc ccgctgaaaa ccggatacgc gggcgaatcg ccggagatgg
     5101 gcttggcggg aaccgagatt gacgaagatt aagaatttaa accacggaga cacggagaca
     5161 cagagaaatg aaaccacaga tttacacaga taaacacaag ataatttaac aggatggaca
     5221 ggatagaagc aggcttcgcg gcttaataaa atctattttt tatctgtctt cgtttttatc
     5281 cgagttaatc cgtgtaaatc tgtggttaaa ttttctcaaa aatctctgcc tctctgcagt
     5341 aaaaatcaaa tgaaaaactt taccgattta tatttggcgc ttgaccagac gaacaagacc
     5401 aacgaaaagg tcgaggcgat ggtcaaatat ttcagcgagg cgacggcgga agacgcggcg
     5461 tgggcgattt attttctgag cgggcgcaag ccgcgacaga ttgttccgac cgcgaaattg
     5521 cgcgcctggg cggcggaaat agcgggcgtc gaagactggc ttttcgagct ttcctatgat
     5581 gccgtcggcg atttgggcga aacgattgcg ctgcttttgc ccgataattc agcgaccgga
     5641 agcgatttgt ctttgagcta ctggattgag aaacgaattc tgccgctgcg ggatttggcg
     5701 gaagcagagc agcacgaggc ggtcgcgcag gcgtggaacg aattggacgc cgaccagcgt
     5761 tttgttttca acaaattcat cacgggcagt ttccgcgtcg gcgtctcgca gcttctggtc
     5821 gttcgcgcgc tggcgcaggt cgccggaatc gaaaaggaaa tcatcgcgca tcgcctgatg
     5881 ggaacgtggt cgccgaacgc cgatttttat ttgaatttga ttgaagccgc cgcggcggac
     5941 gccgatttgt cgcgcccgta tccgtttcat ctggcgcacc agattgattt tccgctcgaa
     6001 gatttgggcg cgatttcgga ttggcaagcc gagtggaaat gggacggcat ccgcgcgcag
     6061 ctgattcgtc gcgcgggcga aagcttcctg tggtcgcgcg gcgaggattt ggtgacggaa
     6121 cgctttcccg aaattacgga agcgagtttt aatctgcccg acggaacggt tctggacggc
     6181 gagattctgc cgtggaaaac gggcgcggtt ttgcctttcg cgcagctgca aaggcgcatc
     6241 gggcgcaaaa caatcggcaa aaaattgctg gaagaagtgc cggtcgtgct tttgtgttac
     6301 gacattctgg agtttcaagg cgaagatatt cgcccgaaac cgatgcgcga gcggcgagcg
     6361 attctcaccg aaattttagg gaatctgccc ggcgaatcta aaaaatcgct tttgcttgag
     6421 ccggttgtcg aggcaaaaga ctggaagaaa ctggcggaaa ttcgagcgga aagccgcaac
     6481 cggaaagtcg aaggctttat gctcaaacgg ctcgattcaa tgtatcgcgt cgggcgaccg
     6541 cgcggcgatt ggtggaaatg gaaaatcgac ccgctgacgg tcgatgccgt tttgatttac
     6601 gcgcagcgcg gcacgggaaa acgcgccagt ctttacaccg attacacttt cgccgtctgg
     6661 cgcgacggcg agctggtccc gtttgcgaaa gcttattcgg gtttgaccga cgcggaaatc
     6721 cgcaaggttg accgttttgt ccgcaacaac acgattgaga gtttcggtcc ggttcgctcg
     6781 gtgacgccgc agctggtttt cgagctcggc tttgaaggca tccagcgctc gacgcggcat
     6841 aaatcgggca tcgccgtccg ctttccgaga attttgcgct ggcgcgacga taaaaaaatc
     6901 gaagacgccg acacgctgga aacgattcac gcgatgctcg acgcggaaga aggaaattga
     6961 accgccgaga cgccgcagac gcggagcaag aaattaacag ggatgaaggg gataaacagg
     7021 gattaataaa aatcattctt tttatccctg ccaatcccct tcagccctgt taatttctct
     7081 ttgaaaaatc tctgcgtctg cggcggtgaa aaaatttatg gagaaagcgc agtatccatt
     7141 tttagttctc acgaccggcg agcgttttcc gcgcctgctg gttacgcggc gtcctgattt
     7201 ggtcggcgaa aaacacacgc tttacggagc ttttctgccc gagacgatgg tgcgccgcct
     7261 gattgatttt atcgcgcgcg tttttcgcct gcacccgtgc gagctggaca ttgacggcag
     7321 cttcgatgcg ccgtgtcccg aatattttct gcgccgctgc cttgcgccgt gcgtcgccga
     7381 aatctgcgac gacgaaaaat acaaaagcgc ggtcgaagcc gttcaactgc ttttgtcgag
     7441 gcgcgccgac gaacttttat ttaaaattga taaagaaatc gggcatttag ccgaaaatct
     7501 ggaatttgaa cgcgcggctg aggcgcgcga tattcgccgc gcgctttccg aaatttttac
     7561 cgattcgaaa tggcagattc gcgccgagga tttcacggac gttttaaccg caacggtcga
     7621 aaacggcgaa gcctcggttc acctgaacac tctgcggcgc gggaaagcgg tcggcggaaa
     7681 gcattccgtc tggaagctcg aaagcgaaaa cggcgatgaa ggcgcggctc tggctgagtt
     7741 tatcagggat ttctatcgat tttatttacc gagacggatt tacgtctcgc acgattttcc
     7801 cgctcgaaaa acactggaaa aacgtttgtc cgagcatttc gggaaaaagg ctgaaataat
     7861 cgccgcgctg cccgaaaagc tgccgccgac ggttttcacc gcgcagaagc gcgccgaaat
     7921 cctgctcgcc aatccggttt tgcaaagcgg gcgcgacgcg gaaaaaatcg ccgcggaatt
     7981 aaaagaaatt tttaatttgg aaaacgcgcc gcgccgcgtc gaatgtttcg acgtggcgca
     8041 tctggcggga aaggaaatcg tcgccgcgcg cgtcgtcggc agaggcggca aattggcgac
     8101 ggatgaagat attgtctggc agtttgaaaa tctgtcggaa accgagagcc tggcggaaag
     8161 cgtgcgcgaa agattaaagc ttttgccttc gcgcgcggat ttgccggatt tgattttgct
     8221 cgacggcgga aagccgcaga tgaatgccgt aaaaagagtt ttgcgggaat ttgatttgga
     8281 aaacctgccg ctcgccgccg ccgtcaaacc gccgagacaa cacgataaag tgagtcattt
     8341 tttaactaat cccggcgagg cgaaaatcga ttttaattcc cgctcgccgg cgatgaattt
     8401 tctgcaaaag ctccgcgacg aagcgcacgc cttagcgaat cggacgcacc gcgaagtcca
     8461 ttcgctcgtt caaatcttcg accggcggat tggctccggc gcgccgaaag ttcagctttt
     8521 gctcgtcccg acgcgctacg ccgcgcgcgg cgggcgagcc gaagatttat cgccgattcg
     8581 ctctctgaat caatcgggcg aaattttgtt taaagccgga aaaaatcaaa gctcagccgg
     8641 cgaaaaacac aaacgccgca gccgtttcag ccgcaaagcg gaataaagtt tttgccgatt
     8701 gttttcgttt cgagcctaga tacttgaata aagtttcgat tttcagtaat aatatcagtt
     8761 cgtctttgat ttatggcggc tatagttcag gggttagaac gccagattgt ggttctggat
     8821 gtcgtgggtt caattcccac tagccgcccc ataaatttta aaataggtat cggcgtcttc
     8881 attttgcctt tgaccttttt actttttcct taatttccct tgtctcacag ccctgaaaaa
     8941 actaaaatat aatgatattc aatatcattc gcgcgaggtt gtaagctgat gaaaaccgta
     9001 aaatttgtat ttcttttcgt tttggcgttt ccgcttttct ctcaaatcgc ttttgcccag
     9061 tataactccc gagtttatgc cgacggcgaa ttgctcgtca agtttaaaga cgggacggtt
     9121 tccgcttcgg cggtttcggc aaactcgcag ttcggcgcgc gcgtggtcga agaatttccc
     9181 gatttgggct ggcagagagt aaagcttccc gccggtttgc cggttgaaac cgccgtcgcg
     9241 cggtacgcaa atctgccgga cgtcgaatac gtccagccga attatctcta tcacctgcaa
     9301 gccacgccga acgacccgca attcagcagc gccggaatgt acggattgac gaagatttcc
     9361 gcgcccgccg cctgggattt gacgattggc agcgcgaacg tcgtcgtcgc caacatcgac
     9421 accggaatca actataatca cgaagacctg gcggcgaata tgtggacgaa tccgggcgag
     9481 ataaacggaa acaacctcga cgacgacggc aacggtttta tcgacgatta ttacggttat
     9541 gatttctttt tcagcgactc gaacccgcag gacgaaaacg gtcacggaac gcacacggca
     9601 ggcactatcg gcgcggtcgg caataacaac gtcggcgtcg tcggcgttaa ctggaacgtt
     9661 cgcctgatgg cgataaaaat ttataattcg agcggtttcg gcacgacctc ggcgatgctt
     9721 atcaacgctt acaattacgt cagattgatg aaaaatcgcg gcgtaaatat ccgcgtgacg
     9781 aacaacagct acggcggctg cgacgaggct tgcggctacg accaggcgac gaaagacgcg
     9841 ctcgacgcgc tgggaaatgc ggggattttg aatgtctttg cggcgggcaa caacggacac
     9901 aatgtcgaaa ccgaagcgtt ttatccggca agctatacca gcccgtcgat tttagcggtc
     9961 gctaactcga cttcgaccga tactcgcgcc ggctcgtcaa attacggagc gatcagcgtc
    10021 gatctggcag cgccgggaac cggaatttta agcacgcata cgaatgtgag cggctacgcg
    10081 aatctgaccg gaacttcgat ggcgtcgccg cacgcggcag gcgcggcggc tctgctttcg
    10141 gcgcgtaatc cgaatctttc gcccgcttct ttaaaagcta ctttgatgaa tacggttgat
    10201 actctcgccg cctggaacgg cgtcgtcaaa accggcggca gattaaacgt cgaccgcgct
    10261 ctgcgcaatc agacggtctg caatttcagc ttgtccgcct acgaatttcg gacgtcgctc
    10321 gacggcggaa acggcagcgt caatgttgcc gctctgacga attgcgatta ctcgattaaa
    10381 agcaacgcgt cctggattgt gatcaattcc ggcaatacgg gaagcggaaa cgcgacaatc
    10441 aattttaccg ttcagccgtt caacgtctcc gacgggatgc gcacgggaac cgtcacgatt
    10501 gccgaccaga cttttttcgt tattcaggga cagccgtttc tgccgcgcaa cctgttggat
    10561 tttgaaggcg acgggcgcac ggattacgtc gccattcaaa acgacaacgg ctcgatgatt
    10621 tggcatatta accgcagctc ttccggttac gtcacgcgaa atttcggatt ttttgccgac
    10681 gacattgccg tgccgaacga ttttgacgcc gacggcgtaa cggacgttgc cgtctggcgc
    10741 aactcgaacg gaacttttta cgttctgcaa agcgcgaaca atacgtttaa gggacagcaa
    10801 ttcggcgcga gcggtgataa tccgaacgtc acgcaggatt ttgacggcga cgcgcgcgcc
    10861 gatttcgccg tcaccagaaa agaaaacgga aagctcgtct ggtatatgct caactcgatt
    10921 accggatttc gcgccatcca gttcggcaac gaaaacgacc gcccgattcg cggcgattac
    10981 gacggcgata acagagccga tttagcggtt tatcgtccgg cggcggattc tccggcaaat
    11041 acttttatcg tgctcggaag cattaacaac gggctgaaaa ctttaacctt cggcaattcg
    11101 gcaacggata aagtcgtgcc gggcgattat gacggcgacg cgaaaaccga ttttgccgtc
    11161 tggcgcacga ccgacgggac gtggtatctg attaaaagct cgacgggcgc gttcagcggt
    11221 ttccaattcg gcgcgagcgg cgatttgccc gcgccgggcg attacgacgg cgacgggcgc
    11281 acg
//
LOCUS       DASWNE010000246         4557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_148368, whole genome shotgun sequence.
ACCESSION   DASWNE010000246 DASWNE010000000
VERSION     DASWNE010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4557
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148368"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="VGB68_05095"
     CDS             <1..715
                     /locus_tag="VGB68_05095"
                     /inference="COORDINATES: protein motif:HMM:NF025877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Wzt carbohydrate-binding domain-containing
                     protein"
                     /protein_id="HEY0048638.1"
                     /translation="LAVGDIAFQKKCLNKMRDVRQQGRTVLFVSHNLQAVSRLCRRVI
                     WLERGKVRADGEAQQVISQYLHAQMNSNSEKVWENPANMPGNEITRLLRVRAASESGE
                     TTSSFDIRRPVFLELTYEVLRDEKIFTPTFHIYNEEGICVFVSNDVTKEWRNRPREKG
                     VYTSRLEIPPNFMAEGSFSVTVVAFTAAPYEEHFFERDVIDFHVFDSLDGDTARGDFG
                     GIMIGVVRPLLHWETDIRK"
     gene            723..1904
                     /locus_tag="VGB68_05100"
     CDS             723..1904
                     /locus_tag="VGB68_05100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0048639.1"
                     /translation="MKNKTDSKILFITPGASRAGGNIFLFNFLKWFKQNSDIPFLTVY
                     GHGGELEEDFKSLSKTFKFNYEPRPESFYSRTTSAIFRRVKWRERLLARQIATEKINL
                     IYANAVTNHQILAALPDLRAPVISHCHELESVIHRTGLAGFEQTKKRTSHFIAVSEAV
                     RKNLIENHGVPFEKISLAHGFIPIESFSAAELEQKNKKIRRQLGIPGNAFVVGASGTM
                     YWRKAPEIFVQIAAAVHRLKPDLPIYFLWVGGAEKGDFRFFEMEYDVKKLGLEKYVRF
                     LEHKPNPMEYYAALDVFAMVSREDPYPLVCLETASLGKPIICFADAGGMPEFVENDSG
                     FIVSYLDIEAFAGRISELYANPGLREKMGARAALKVRERHDILTSAPGIVRVINKVLN
                     G"
     gene            1906..3627
                     /locus_tag="VGB68_05105"
     CDS             1906..3627
                     /locus_tag="VGB68_05105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0048640.1"
                     /translation="MNLQRKIKNAVRTWRNDGSQGLINLLEIKFRSLKEKRKYQKWVR
                     EFDALTGEDRRKIRRKIESFPRQPLISVILPVYNIDEKWLRLCIESVRRQLYANWELC
                     IADDCSPSPHVRKILAEYAARDERVKVVFREKNGHISAASNSALELATGEFCVLLDHD
                     DELAEHALYFVAEELNLFPETAMIYSDEDLIDSKGRRYEPKFKPDWSRDFICSLNLIT
                     HLSAYRTEILRRVGGFKIGAEGSQDYDLALRVTELISADRIRHIPQILYHWRAIQGSV
                     ALSGDEKPYAAERAREAIRAHLQRTGKKAQVSQTFHNLHRVSYDLPADLPKVSLIIAA
                     KDEKNLPSLAAKTDYENLQFLSQTAAGQSSAAGFNHLAEISGGDILVFLDGDFEPQNS
                     GWLKEIIGFARQAEIGAVGAKIINPNERIRHAGVVLGVDGFIGLAHRGLPKNSDGYLQ
                     RAQLVNNFSAVANVLATRREVFESLAGFDAENFPNGLFDVDFCLRLRAKNLRVVLTPY
                     AEILQTSDSSTEKILNQKDAAEIEKFKTRWKDLIANDPFYNPNLSLENEKFEINFPPR
                     VKKPWQD"
     gene            complement(3624..4079)
                     /locus_tag="VGB68_05110"
     CDS             complement(3624..4079)
                     /locus_tag="VGB68_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY0048641.1"
                     /translation="MTRELILDLPLEKVFGFFADAGNLERITPPQLNFHIITPQPIDL
                     KKGALIDYRLKLRGVSLTWRTVISTWNPPFEFVDEALKSPYKQWIHRHTFSVAGENET
                     LIKDEVRYRLPFEPLGDIAHFFVRKELNYIFDFRQKTVKEILQTEKFSD"
     gene            complement(4212..>4557)
                     /locus_tag="VGB68_05115"
     CDS             complement(4212..>4557)
                     /locus_tag="VGB68_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048642.1"
                     /translation="ARLIPTGKNPNTGVYGARAMTPDDYIKRNEPFFVKEGFYEREMA
                     RRIDLYGNIAHVFSTYESFHKKDDTTPFMRGINSIQLLNDGKRWWIMTIYWQGETPET
                     PLPKEYLKSKKN"
BASE COUNT         1142 a         1096 c         1185 g         1134 t
ORIGIN      
        1 tttagccgtc ggcgacatcg cttttcagaa aaaatgcctc aacaaaatgc gcgacgttcg
       61 ccagcagggc agaacggttt tgttcgtttc gcacaattta caggctgtct cgcggctttg
      121 ccgccgcgtc atctggcttg agcgcggaaa agttcgcgcg gacggcgagg cgcagcaggt
      181 tatcagtcaa tacctgcacg cgcagatgaa ttccaacagc gaaaaggttt gggaaaatcc
      241 ggcgaatatg ccgggaaacg aaattacgag attgcttcgt gttcgcgccg cttccgaatc
      301 cggcgaaacg acttcgtcct ttgatattcg ccgtccggta tttctggagc tgacttatga
      361 agttttgcgg gacgagaaaa tttttacgcc gacctttcat atttacaacg aagaaggcat
      421 ctgcgtcttc gtctccaacg atgttacgaa agaatggcga aatcgcccgc gcgaaaaagg
      481 agtttacacg agccggctcg aaattccgcc caattttatg gcggaaggaa gtttttccgt
      541 gacggtcgtc gcttttacgg cggcgcctta cgaagaacat ttttttgaac gcgacgtgat
      601 tgattttcac gttttcgaca gtctggacgg cgacacggcg cgcggcgatt tcggcgggat
      661 tatgatcggc gtcgtgcgcc cgcttcttca ttgggaaacg gatatccgca aataattgag
      721 cggtgaaaaa caagactgat tcaaaaatac tttttatcac gccgggcgct tcgcgagcgg
      781 gcggaaatat tttcctgttt aattttttaa aatggtttaa gcaaaattcc gacattccgt
      841 ttttgaccgt ttacggtcac ggcggcgaat tggaagaaga ttttaaaagc ttgtcaaaaa
      901 cttttaaatt taattacgag ccgcgcccgg aaagttttta cagccgcacg acgagcgcga
      961 tttttcggcg cgtaaaatgg cgcgagcgtt tactggcgcg gcagatagcg acggaaaaga
     1021 ttaatttgat ttacgccaac gccgtcacga atcatcaaat tctagccgct ctgcccgatt
     1081 tgcgcgcgcc cgtaatctcg cattgtcacg aattggaaag cgtcattcac cgcaccggct
     1141 tagcgggttt cgagcaaaca aaaaaacgca cttcgcattt tatcgccgtc tcggaagccg
     1201 tgcggaaaaa tctaattgag aatcacggcg ttccgtttga aaaaatcagc ctggcgcacg
     1261 gatttattcc gattgaaagc ttttctgccg ccgagctgga gcagaaaaat aaaaaaatcc
     1321 gccggcagct cggaattccc ggaaatgctt ttgtcgtcgg cgcttccggc acgatgtatt
     1381 ggcggaaagc gcccgagatt tttgtacaga ttgccgccgc cgttcatcga ctgaagcccg
     1441 acctcccgat ttatttcctt tgggtcggcg gcgcggaaaa gggcgatttt cgtttttttg
     1501 aaatggaata cgacgtcaaa aaactcgggc tggaaaaata cgtccgcttt ctggaacata
     1561 aaccgaaccc gatggagtat tatgccgcgc tcgacgtttt tgcgatggtc tcgcgcgaag
     1621 acccgtatcc gctggtttgt ctggaaaccg cttcgctcgg aaagccgatt atttgtttcg
     1681 ccgacgcggg cgggatgccg gaatttgtcg aaaacgacag cggctttatt gtttcttatc
     1741 tggacatcga agccttcgcc ggacgaattt cggagcttta cgccaatccg ggtttgagag
     1801 aaaaaatggg cgcgcgcgcg gcgctaaagg tgcgcgaaag acacgacatt ttgacttcag
     1861 cgccgggaat cgtccgagta ataaataaag ttttgaacgg ctaaaatgaa tcttcagcgc
     1921 aaaatcaaaa acgccgtccg gacgtggcgg aacgacggct ctcagggatt gataaattta
     1981 ttggagataa aatttcgttc gctgaaggaa aaacgcaaat atcaaaaatg ggttcgcgaa
     2041 tttgacgctt taaccggcga agaccgccgg aaaatccgcc ggaaaatcga aagttttccc
     2101 cgtcagcctt tgatttccgt tatcctgccc gtttacaaca tcgacgaaaa atggcttcgc
     2161 ctgtgcatcg aatcggtgcg cagacagctt tacgcaaatt gggagctctg catcgccgac
     2221 gattgctcgc cgtcgccgca cgtgcgaaaa attctggcgg aatacgcggc gcgggacgag
     2281 cgagttaaag tagttttccg cgaaaaaaac gggcatattt ccgccgcttc gaattccgct
     2341 ctggaattgg cgaccggcga attttgcgtg cttctcgacc acgacgacga gcttgccgag
     2401 cacgctcttt attttgtcgc cgaggaatta aatctttttc ccgaaacggc gatgatttac
     2461 agcgacgaag atttaattga ttcgaaagga aggcgttacg aaccgaaatt caagcccgat
     2521 tggagccggg attttatctg ttcgctgaat ttaatcacgc atttatcggc gtaccggacg
     2581 gaaattttgc ggcgcgtcgg cggttttaaa atcggcgccg aaggcagtca ggattacgat
     2641 ttggctttgc gcgtgacgga actgatttcc gccgaccgca tccggcatat tccgcaaatt
     2701 ctttatcact ggcgggcgat tcaaggctcg gtcgccctga gcggcgacga aaaaccttac
     2761 gcggcggagc gcgcccgcga agcgattcgc gctcatctgc aaagaaccgg aaagaaagcg
     2821 caggtcagcc aaacttttca taatcttcac cgcgtcagct atgatttgcc cgccgatttg
     2881 ccgaaagtca gcttgataat agcggcgaaa gacgagaaaa atttaccgag tctggcggcg
     2941 aaaaccgatt acgaaaatct ccagtttttg tctcaaaccg ctgccggtca atcgagcgcc
     3001 gcgggtttta atcacctggc ggaaatatcg ggcggcgata ttttggtttt tctggacggc
     3061 gatttcgagc cgcaaaattc cggctggctg aaagaaataa tcggattcgc acgacaggcg
     3121 gaaatcggcg cggtcggcgc gaaaatcatc aatccgaatg aaaggattcg acacgccgga
     3181 gtggttttag gcgtcgacgg ttttatcggc ttggcgcatc gcggtttgcc gaaaaacagc
     3241 gacggctatt tgcaaagagc gcagctcgtc aacaatttct cggcggtcgc caatgtgctg
     3301 gcgacgcggc gcgaagtttt cgaatcgctg gcgggatttg acgcggaaaa ttttccgaac
     3361 ggtcttttcg acgtcgattt ttgcctgcgc ctgcgggcga aaaatctgcg cgtcgttttg
     3421 acgccttacg cggaaattct gcagacttcg gattcgtcga ccgaaaagat tctgaatcaa
     3481 aaagacgcgg cggaaatcga aaaatttaaa actcgctgga aagatttaat cgcaaacgac
     3541 ccgttttaca atccgaactt atcgctcgaa aacgaaaaat ttgagattaa ttttccgccg
     3601 cgtgtgaaaa aaccctggca ggattaatcg gaaaattttt ccgtctgcaa aatttctttc
     3661 acggtttttt gccggaaatc gaaaatgtaa tttaattctt tccgcacgaa aaaatgcgcg
     3721 atgtcgccga gcggctcgaa aggcaggcga taacgcactt cgtcttttat taaagtttcg
     3781 ttttcgcctg cgacgctgaa agtgtggcga tgaatccatt gtttataagg actttttaaa
     3841 gcttcgtcga caaattcaaa cggcggattc caggtcgaaa tcacggttcg ccacgtcaaa
     3901 gaaacaccgc gcagctttag ccggtaatcg attagagcgc cttttttcag gtcaatcggc
     3961 tgcggcgtga tgatgtgaaa attaagctgg ggcggcgtga ttcgttccag atttccggcg
     4021 tcggcaaaaa aaccgaagac tttttcgagc ggtaaatcca gaattaattc tcttgtcaaa
     4081 atatgctctg ccataagcaa agattgacaa agctccgagg gaaaatcaaa caggatgaac
     4141 cgggtaaaca ggttatttcg agcaccactc gataacctgt ttacccggtt catcctgttt
     4201 cgatccggac gctaattctt tttacttttc agatattctt tcggcagcgg cgtttccggc
     4261 gtttcgcctt gccagtaaat cgtcataatc caccagcgct tgccgtcgtt cagaagctgg
     4321 atgctgttga tgccgcgcat aaaaggcgtc gtgtcatctt ttttgtgaaa agattcgtag
     4381 gtcgaaaaaa cgtgcgcgat gttgccgtaa aggtcgattc gccgcgccat ctctctttcg
     4441 tagaaacctt ctttgacgaa aaacggctcg ttgcgtttga tatagtcgtc gggcgtcatc
     4501 gcgcgcgcgc cgtaaacgcc ggtgttcgga tttttgccgg tcggaatcag tcgggcg
//
LOCUS       DASWNE010000247         4100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_394655, whole genome shotgun sequence.
ACCESSION   DASWNE010000247 DASWNE010000000
VERSION     DASWNE010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394655"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            276..1151
                     /locus_tag="VGB68_05120"
     CDS             276..1151
                     /locus_tag="VGB68_05120"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0048643.1"
                     /translation="MSENPKEKEPQPPFPEQQQTPPGLESEMNPAPDFGERSYKGTGR
                     LQDKAAIITGGDSGIGRAVALAFAREGADVLISYLSEEEEDAQKTRRVVEEAGRRCVT
                     MPGDIREEAHCREIIKRAMDEFGKIDVLINNAAFQMSHEGIGEISSEEFDRTFRTNVY
                     AMFYLCKAALPLMKAGSTIINTTSIQAYEPKPHLLAYAATKGAIMNFTKGLNHEALEK
                     GIRVNAVAPGPIWTPLIPATMPPEKTATFGQDTPMGRAGQPAELAPVYVFLASDESSY
                     ISGEIIGVTGGKPLP"
     gene            1380..1823
                     /locus_tag="VGB68_05125"
     CDS             1380..1823
                     /locus_tag="VGB68_05125"
                     /inference="COORDINATES: protein motif:HMM:TIGR04191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HEY0048644.1"
                     /translation="MPYPEIMIHGMRQELAQLGIEETKTPEAVKDAVEKTEGTLMVVV
                     NSVCGCAAGGVRPGIAMALQNAEAKPDKAITVFAGADIDATDVARDYFTGYQPSSPAI
                     GILKDGKLVQMFERRDLEGRHPLQIAQTLIDAFNAHCTTSNAASN"
     gene            2058..2513
                     /locus_tag="VGB68_05130"
     CDS             2058..2513
                     /locus_tag="VGB68_05130"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY0048645.1"
                     /translation="MSIEQRRHIRFSLDIPAIRQDETGERHPIMLHQISVGGCLTSLD
                     ENIYTGDEFRVLLRLPSGNFLPLVCKALYKFAGSGIGAKFVEITEFEQQLLADVISQT
                     LEAEGLPLQVDPFAHPPTQIYQNATREEKLANPRIEKEEIVEQILSIDN"
     gene            complement(2609..3079)
                     /locus_tag="VGB68_05135"
     CDS             complement(2609..3079)
                     /locus_tag="VGB68_05135"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0048646.1"
                     /translation="MKLKVFIFLLLFSSISVFAQSKDEATLKSLVKQMTDAQMSYQPE
                     ALDKIFAADYIEISPVGEFDPREKVLGFYKPEMKPDSSKMTATVEATDFSIRHYDKFA
                     IVITRLNYTRIAGGKTLPPVSMRATIVCRKEKGEWKIVSAQYTGIRPPQPAPKS"
     gene            3253..3903
                     /locus_tag="VGB68_05140"
     CDS             3253..3903
                     /locus_tag="VGB68_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048647.1"
                     /translation="MKLKRIFVWIWLSFSLVYFPVNAQTANNKKSGTPKNTGSQCLVK
                     GLSFDCPDDFVKDDEIGDKTLIFKGIAGSLLTYLFVSTSPGFADKAEIQKRIAGKFNS
                     SKPDKFVWKKVESSLFMNLRTKYKKSESSFFGFNDDILLNLINRQFLFKNQEIYIGYV
                     YEMKDSDPKSQFENPLGGENAGGCNAVVTLLNSITKESKEKKQYCYLQLEIGAVQK"
BASE COUNT         1172 a          943 c          953 g         1032 t
ORIGIN      
        1 taaacaaatc ttactgacga gcaagccgct ttccgggatt ttccataaac tgtaattcgc
       61 tttccggcaa aatcgataac ttaaaaaacg cgcataagac gaaaaaagtt ctgtgccgaa
      121 gccgtagctg tgttcgctaa gctacagaaa tcgctcgccg gatatgttta gattgttcca
      181 tcaatatttg aaataatttg aaataaaata aaggaataaa gcttaaagct tttcaatctg
      241 aaaagagaag ctttaaagca aaaggtattt tttatatgtc ggaaaatccg aaagaaaaag
      301 aaccgcagcc gccgtttccc gagcagcagc agacgccgcc ggggctcgaa tccgagatga
      361 atccggcgcc ggatttcggg gagcggagct ataaaggcac aggcagattg caggacaaag
      421 cggcgattat caccggcggc gattcgggca tcgggcgcgc cgtcgcgctg gcttttgccc
      481 gcgaaggcgc ggacgtattg atttcttatt taagcgaaga ggaagaagac gcgcagaaaa
      541 cccgccgcgt cgtggaagaa gccgggcggc gctgcgtcac tatgccgggc gacatccgcg
      601 aagaagcgca ctgccgcgaa attataaaac gcgcgatgga cgaattcggt aaaatcgacg
      661 tgctgattaa caatgccgcg tttcagatgt cgcacgaagg aatcggggaa atttcgtcgg
      721 aagaattcga ccgcacgttt cgcaccaatg tctacgcgat gttttatttg tgcaaagccg
      781 ccctgccgct gatgaaagca ggcagcacga ttatcaacac gacttctatt caggcttacg
      841 agccgaagcc gcatctgctg gcttatgcgg cgacgaaagg cgcgattatg aatttcacga
      901 aaggactcaa tcacgaagcc ctggaaaagg gaattcgcgt caacgccgtc gcgcccggtc
      961 cgatctggac gcctttaatt ccggcgacga tgccgcccga aaaaaccgcg actttcggtc
     1021 aggatacgcc tatggggcgt gccggacagc cggcggaact ggctccggtt tacgtctttc
     1081 tcgcttcgga cgaatccagc tacatcagcg gcgagataat cggcgttacc ggcggcaaac
     1141 cgcttccata aaattttgaa aaaccggcgt ttaaaatcac agcccataat cggctgtgat
     1201 tttttttgcg gtcgtccgcg ccgtttcgcg cgattacgga agcgcgcctc atttttattt
     1261 tcgcgttgat attttcacct tttgctttaa caaacaaatt ctgctgcttg acgaaagcgt
     1321 gatagatact ggaaaataaa gtaaagatta tattttgata ttttgagagg ttaaattaaa
     1381 tgccatatcc agaaattatg attcacggaa tgcgacagga actggcgcag ctcggaatcg
     1441 aagaaaccaa aacgccggaa gcggtaaaag atgcggtcga aaagacggaa ggaactttga
     1501 tggtcgtcgt caattcggtc tgcgggtgcg cggcgggcgg cgttcgtccc ggcatcgcga
     1561 tggctttgca gaatgcggaa gccaaaccgg acaaagccat tacggtcttt gcgggcgcgg
     1621 acatcgacgc gaccgacgtg gcgcgcgatt attttacggg ttatcagccg tcgtcgccgg
     1681 ctatcggcat tttgaaagac ggaaagctcg tccagatgtt tgagcgccgc gacctcgaag
     1741 gtcgtcatcc gcttcagatt gcgcagactt taatcgacgc tttcaacgcg cattgcacga
     1801 cttcaaacgc cgcttcaaac taaacaaatc aaagtggtaa acggtaaacg gtaaacggta
     1861 agttgcagtt gaaaagtatc ggatttttat ttaaaactcg atattcaaaa caacgaactt
     1921 accgtttacc gtttaccgtt taccgccgct tcccactttt ttgttgcttt tccccaaaag
     1981 aaacctgtaa aatagctttt gacaacggtt ttacgccgtt cggtaacctt ccgaaaaact
     2041 tttcaggttt ctctccgatg tcaatcgaac aaagacgcca tatcagattt tctctcgaca
     2101 ttccagcgat tcgccaggac gaaaccggcg aaaggcaccc gattatgctt catcaaatca
     2161 gcgtcggcgg ctgcctgacc agccttgacg aaaacatcta cacgggcgac gaatttcggg
     2221 ttttgctgcg tttgccgagc ggcaattttc tgccgctcgt ctgcaaggcg ctttataaat
     2281 tcgccggttc gggcatcggc gcaaaatttg tggaaatcac cgaattcgaa cagcaattgc
     2341 tcgccgacgt aatctcgcaa acgctcgaag ccgaaggctt gccgcttcag gtcgacccgt
     2401 tcgctcatcc gccgactcaa atctaccaga acgccacccg ggaagaaaag ctcgccaatc
     2461 cgcgcatcga gaaagaagaa atcgtcgagc agattctttc catcgataat taaagacgtc
     2521 cgataatttt tgaaaacgac ggcgaaaaat aataaaaggc tgtgagaaat tctcacagcc
     2581 ttttatattt ttatgatgat tcgtcttatc aggatttagg cgcgggctgc ggcgggcgga
     2641 ttccggtgta ttgcgccgag acgattttcc attcgccctt ttctttgcgg cagacgattg
     2701 tcgcgcgcat actgaccggc ggaagcgttt tgcctccggc gattcgagtg taattcaggc
     2761 gcgtgatgac gatggcgaat ttatcgtaat ggcgaatcga aaaatccgtc gcttcgaccg
     2821 tcgccgtcat tttcgacgaa tcgggtttca tttcgggttt gtaaaatccc agaacctttt
     2881 cgcgcgggtc aaactcgccg acgggcgaaa tctcgatgta atcggcggcg aaaattttat
     2941 cgagcgcttc gggctgataa ctcatctgcg cgtcggtcat ttgtttgacg agcgatttta
     3001 aagtcgcttc gtctttcgat tgagcgaaaa cggaaatgct cgaaaagagc agaagaaaaa
     3061 taaaaacttt cagtttcata aacgaactcc tcaagaaaat aaaataaaaa ccgtgtgttt
     3121 tacgtctcaa attttatttt agtttcacct tgacctatgt tgcccgcgcc ggctttcagc
     3181 agtccgaagt aaatcagaaa tttgactcga aacaaaaaaa cttccaaaat aacttacatt
     3241 ttggaaaaag atatgaagtt aaaacgaatt ttcgtctgga tttggctgag cttttcatta
     3301 gtttattttc ccgtaaatgc tcagactgca aataataaaa agtcgggaac gccgaaaaat
     3361 accggctctc aatgtctggt aaaaggctta agcttcgatt gtccggacga tttcgtgaaa
     3421 gatgatgaaa taggcgacaa aactctcatt ttcaaaggca tcgcgggcag tttattgacg
     3481 tatctcttcg tttccacttc gcccgggttc gccgataaag cagaaattca aaaaagaatc
     3541 gccggaaaat tcaattcctc caaaccggac aaatttgtct ggaaaaaggt tgaaagctct
     3601 ttatttatga atttgcgaac caaatacaaa aaaagtgaaa gcagcttctt tggttttaac
     3661 gacgacattc tgttaaatct tatcaaccgg caattcttat ttaaaaatca ggaaatatat
     3721 atcggttatg tatatgaaat gaaagactcc gaccccaaaa gccagtttga aaatccgtta
     3781 ggcggcgaga acgccggcgg ttgtaatgcg gtcgttacgc tccttaattc gattaccaaa
     3841 gagagtaaag agaaaaagca atattgttac ttacagctcg aaataggcgc ggttcaaaaa
     3901 taatttaaat tgtcactgct gatttcataa agcgttttta ccattgtcgc gagctttgca
     3961 caaaaataaa gaaaatcgag aaaaattccg atgagtaatt gtaaattctc aattatcaat
     4021 tctaaattac ttcacaaaag ataccgaaca tcaatttaga atttacaatt tacaatttac
     4081 aattttccct ttctttattt
//
LOCUS       DASWNE010000248         3022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_255470, whole genome shotgun sequence.
ACCESSION   DASWNE010000248 DASWNE010000000
VERSION     DASWNE010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255470"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VGB68_05145"
     CDS             complement(<1..473)
                     /locus_tag="VGB68_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048648.1"
                     /translation="MNYTKMNYRQHRYVFFVLALFSCLFLPIAVEAATRTWDGGGVTN
                     NWSEAANWSDDIAPVNGDETIFNAAGTKNATIDVSVSVFNFFIQSGYTGTITQAGGTT
                     VTTTFQFTQSAGTYVGGSGAFNFPGAFLTIQNTAAFQGGTGLISGNRMTQNGGAFT"
     gene            730..1296
                     /locus_tag="VGB68_05150"
     CDS             730..1296
                     /locus_tag="VGB68_05150"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0048649.1"
                     /translation="MLPQHQVTNYLREYRSGNREVLDELFPLVYEELRKLAANRLRGE
                     RDDHTLQPTALVHEAYLRLIEQHTQNWQNRAHFFGLAAEMMRRILINYANNRNAEKRF
                     GNRTRIALDELVTFSEQRDVNLIQLDEVLKELAGFDEKQARIVELKFFGGLTNEEIAE
                     VLGVSDSTVKREWRIARAWLSERLKAEG"
     gene            1300..>3022
                     /locus_tag="VGB68_05155"
     CDS             1300..>3022
                     /locus_tag="VGB68_05155"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0048650.1"
                     /translation="MKAEEWEKVKEIFNRAVELPPVERERFLASFQNGDAYLRREVEK
                     LLAADENSGAQFEDFSFVSAGNLKEKIGRYKILREIGAGGMGTVYLAEREDLAQKVAL
                     KIIRHGANSDVVVRRFRKEQEILAALEHANIARLLDVGLSDEGLPFLAMEYIEGSDLL
                     AYCAENNLSLDAKLKLFRKICDAVAYAHSRLVVHRDLKPSNILVNSKGEPKLLDFGIS
                     KLLSENNREEKGTVTSFGMLTPNYASPEQFRGESVSTAADVYSLSVILYELLTGKLPY
                     DVENRRYDEVARIVCEIHPPKPSDILTQSRKDAETGGAARTTDKGRFFKGHRTKDNGR
                     SLKGDIDNILLKALRKEPERRYSSVEKFSDDLRLHLEGLPVTARPDTFSYRAEKFVRR
                     NRVAVASAGLMILIFLAGVGGIMWQYFRAERQRILAERRFSDVRQLANNVVFKYHDEI
                     QNLPGSTRAREMLVKDALVYLNRLALEEDAETSDVSLKLELAQAFMKIGDVQGQAYDA
                     NLGDTAGAVESYRKAVALLENAAAQNPGDLKIENELATSYQKISSLIGRAGTQRESFG
                     FAEKAVAL"
BASE COUNT          755 a          778 c          828 g          661 t
ORIGIN      
        1 gtgaaagcgc cgccgttctg cgtcatccgg ttgccgctga tcaaacccgt tccgccttga
       61 aaagccgccg tgttttggat tgtcaggaaa gcgcccggaa aattaaacgc gccgctgccg
      121 ccgacgtaag ttccggcgga ttgagtgaat tgaaacgtcg tcgtgaccgt cgtaccgcct
      181 gcctgcgtga ttgtgccggt gtagccgctt tgaataaaga aattaaagac gctgacgctg
      241 acatcaatcg tcgcgttttt cgtgccggcg gcgttaaaaa tagtttcgtc gccgttgacc
      301 ggcgcgatat catccgacca gttggcggct tccgaccaat tgttcgtcac gccgccgccg
      361 tcccacgttc gggttgcggc ttcgacggca atcggaagaa agagacacga gaaaagcgct
      421 aacacgaaaa aaacgtagcg gtgttgtcta taattcattt ttgtataatt catttttttt
      481 tccttcaagc gttatgtttg ataattttcg aattgagcgg aaacgcccgt ctgaatacaa
      541 acgcaggatt gaggagcgga aaggggcagc ttgcggcaaa aaaaagtttc cggctttcat
      601 acaaagcaac tcggagtttt ggcgcgtaaa atgagcctga tttgtcgtcg cctgagcggc
      661 gagcctcgaa aattttgaaa attcttcgat tgtctgtaaa ataaaacggc tcaaatataa
      721 tcgccgaata tgcttcctca acatcaagtc acaaattacc tgcgcgagta tcggagcggc
      781 aatcgcgaag tgctggatga attatttccg cttgtttacg aagagctgcg aaagctggcg
      841 gcaaatcgac tgcgcggcga gcgcgacgac cacactttgc agccgacggc gctggttcac
      901 gaagcttatt tgcgcctgat cgagcagcac acgcaaaact ggcagaatcg cgcgcatttt
      961 ttcggtttgg cggcggagat gatgcgtcgg attctgataa attacgcgaa taatcgcaat
     1021 gccgagaagc gtttcggcaa ccggacgcgc atcgcgctcg acgaactggt cactttttcc
     1081 gaacagcgcg acgtaaactt gattcagctg gacgaagtct taaaggaact agccgggttt
     1141 gacgaaaaac aggcgcgcat tgtcgagctg aaatttttcg gcggtctgac gaacgaggaa
     1201 atcgccgagg ttctgggcgt ttcggattcg accgtcaagc gcgagtggcg gatagcgaga
     1261 gcgtggctct ctgaaaggct gaaggccgaa gggtaaagga tgaaggcgga agaatgggaa
     1321 aaagtaaaag agatttttaa tcgcgcggtc gagttgccgc ccgtcgagcg cgagcggttt
     1381 ctggcgtctt ttcaaaacgg cgatgcttat ttgcgccgcg aagtcgaaaa gcttctggcg
     1441 gcggacgaaa attccggcgc gcagtttgag gatttttcgt ttgtttcggc gggaaattta
     1501 aaagaaaaaa tcggcagata caaaatcctg cgcgaaatcg gcgcgggcgg aatgggcacc
     1561 gtctatcttg ccgagcgtga ggatttggcg caaaaagtcg ctttgaaaat catccgacac
     1621 ggcgcgaatt ccgacgtcgt cgtgcggcgt ttccgcaaag aacaggaaat tttagccgcg
     1681 ctcgaacacg cgaatatcgc gcggctgctc gacgtcggct tgagcgacga aggcttgccg
     1741 tttctggcga tggaatatat cgaaggctcg gatttgctcg cctactgcgc cgaaaacaat
     1801 ctttcgctcg acgccaagtt aaagcttttc cgaaagattt gcgacgccgt cgcctacgcg
     1861 cattcgcggc tggtcgtgca tcgcgattta aagccttcaa atattctggt caattccaaa
     1921 ggcgagccga aactgctcga tttcggcatt tccaagctgc tctccgaaaa caatcgcgaa
     1981 gaaaaaggaa cggtcacttc gttcggaatg ctgacgccga attacgcttc gcccgaacag
     2041 tttcgcggcg aatcggtctc gaccgccgcc gacgtctaca gcctgagcgt tattctctac
     2101 gagcttctga ccggaaaatt gccttacgac gtcgaaaacc ggcgctacga tgaagtggcg
     2161 cgcatcgtct gcgaaatcca cccgccgaaa ccgagcgata ttttaacgca aagccgcaaa
     2221 gacgcggaaa ccggcggcgc ggcgcgcaca accgataaag gacggttttt taaaggacac
     2281 agaacaaagg acaacgggcg cagcctgaaa ggcgacatcg acaatattct tttaaaagcc
     2341 ctgcgcaaag agcctgaaag aagatattcg tcggtcgaaa aattttccga cgatttacgc
     2401 ctccacctgg aaggcttgcc cgtcacggcg cgccccgata ctttttctta ccgcgccgaa
     2461 aaattcgtca ggcgaaatcg cgtcgcggtc gcctccgccg gtctgatgat tttaattttt
     2521 ctcgccggcg tcggcggcat aatgtggcaa tattttcgcg ccgagcgcca gagaatatta
     2581 gccgaacgcc gtttctcgga cgtgcgccag ctcgccaata acgtcgtttt caaataccac
     2641 gatgaaatac aaaatctgcc cggctcgacc agggcgcgtg aaatgctcgt taaagacgcg
     2701 ctcgtttatc ttaatcggct cgcgctcgaa gaagacgccg aaacgagcga cgtttccctg
     2761 aaactcgaat tggcgcaggc ttttatgaaa atcggcgacg ttcaggggca agcctacgac
     2821 gccaatctcg gcgacacagc cggcgccgtc gaaagctacc gcaaagcggt cgcgctgctc
     2881 gaaaacgcgg cggcgcaaaa tcccggtgat ttgaaaatcg aaaacgagct ggcgacgtct
     2941 taccaaaaaa tttcctcttt aatcggtcgc gccggaactc aacgggaaag cttcgggttt
     3001 gccgaaaaag ccgtcgcgct cg
//
LOCUS       DASWNE010000249         4236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_255629, whole genome shotgun sequence.
ACCESSION   DASWNE010000249 DASWNE010000000
VERSION     DASWNE010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255629"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VGB68_05160"
     CDS             <1..649
                     /locus_tag="VGB68_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY0048651.1"
                     /translation="GRVENFEYKPKNPKNDLITTEIFIYEARVLIETLKKLKETKDEI
                     KDYGHELIPFLVEQGNVFEHRLNGYWRDVGTIKSYWQSQIDLLDEKTKFIFDDERWRI
                     LTLAPQVVPAFIYGSADVKNSLVAQGCRLYGKVSRSVLGSGVTVEKGSEVKDSIVLPQ
                     AIIEKGVKIERVIVDAGVKITKEKMKKIEAARGRDKNAIIVVGKYKLQREKEIEE"
     gene            726..2135
                     /locus_tag="VGB68_05165"
     CDS             726..2135
                     /locus_tag="VGB68_05165"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0048652.1"
                     /translation="MKKPIFKLKAICFLIVLIFLSNASAQESALTTEEGKRLLAEIQV
                     SLRAEKHDEVIEKSTRLLAIAPKSADVYILRGTSFLVKGEKEKAINDFSQGLQFGLSP
                     QMEIAARKMRAIMLFQTKKYDEAIPDYSFLIQKESSDFRGYVQRGWCFFYKGNHQAAI
                     ADFDSSLKLSPQEKGIRRYRAISHYRLGNYEKAVEDASEEIKINPGALAEVYKIRADA
                     YRKLGKIELAESDEKKFAEMSAKPSSAGEVPPKSNFQAKFEELIEKSQNHFRKGEWDA
                     AIQAYNQIIELLGESDKALYAIYFSRGRCFYEKGNLDRALADYNEVIKRRPDLAGGFT
                     FRGEVYLRRNELDSALDDFTKAIKLDSNDILAYRRRAEVYNLKKDYDNAVKDCGDVIR
                     LDPKYQSAWYLRADSYFEKGDFKAALKDISEYIRLDDTSAKAYSLRAKTYRKLNDEKA
                     AAADEKRAAELNNGRQSHE"
     gene            2128..2616
                     /locus_tag="VGB68_05170"
     CDS             2128..2616
                     /locus_tag="VGB68_05170"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEY0048653.1"
                     /translation="MNEKISAVVITASDSREEKDDVSGVTLVGLLLAAGAEVVDKIIV
                     SDDAEKIVETLKAQAARDDVNLILTTGGTGLAPRDNTPDATRAVIEKEVPGMAEAMRI
                     GTLQKTPMSMLSRGVCGTRGKCLIINLPGSPNGVRECFDIIKPVLQHAVNLLAGKSEH
                     KN"
     gene            2859..3314
                     /locus_tag="VGB68_05175"
     CDS             2859..3314
                     /locus_tag="VGB68_05175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsnF/AvaK domain-containing protein"
                     /protein_id="HEY0048654.1"
                     /translation="MANPAESVNTEAQQTQAQADNAAIVVPIIQEEVTFDKKIVETGK
                     VRISKRVSQHEQLIDVPFMREQVDVERVPINQVVDAAPLVRQEGDTMIIPVVEEQIFY
                     QKRLVLVEELRVRKQIIEEHKPQQVTLLKEEVEINRSVENENLSREKGI"
     gene            3346..4215
                     /locus_tag="VGB68_05180"
     CDS             3346..4215
                     /locus_tag="VGB68_05180"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsnF/AvaK domain-containing protein"
                     /protein_id="HEY0048655.1"
                     /translation="MIHTVVGLFDNRSNAGAATQELIQKGFLRENIDVSNRQISDAAT
                     STQIAVTETGVSESIGNFFNSLFGGDKKTARNYTTAAGDADAIITVQVDSLERAREAA
                     EILDRHGAIDVDGRSSQHEQNLQQNQQILTAAPNAPQTAQNAADIHNAANIQNETAIP
                     VIEEQLQVGKQIVQREGARVRSRIVEKPVEANLRLREEHIVVNRRSVNRDVTNADLAN
                     FREGELVITEHAEIPVVGKQARVVEEILIGKNVTEHVETVHGTIRRNEVEVEKVNTDV
                     TDADDINTRGATT"
BASE COUNT         1271 a          987 c         1083 g          895 t
ORIGIN      
        1 cggacgcgtc gaaaatttcg aatacaaacc gaaaaatccg aaaaacgatt taatcacgac
       61 cgagattttc atttacgaag cgcgggtttt gattgaaacg ctcaagaaat taaaggaaac
      121 aaaggacgag ataaaagatt acggtcacga gctgattcct tttttagtcg agcagggcaa
      181 tgtcttcgaa catcgtctga acggctactg gcgcgatgtc gggacgatta aaagctactg
      241 gcaatcgcag atcgaccttc tcgacgagaa aacgaaattt atctttgacg acgagcgctg
      301 gcggattttg acgctcgctc cgcaggtcgt tccggctttt atttacggct cggctgacgt
      361 gaaaaacagt ctcgtcgcgc agggctgccg cctttacggg aaagtttcgc gctcggttct
      421 gggctcgggc gtgaccgtcg aaaaaggctc ggaagtaaaa gattcaatcg ttctgccgca
      481 ggcgataatc gaaaaaggcg taaaaataga gcgcgttatt gtcgatgcgg gcgttaaaat
      541 aaccaaagaa aaaatgaaaa aaatcgaagc ggcgcgcggg cgcgataaaa atgcgattat
      601 cgtcgtcggc aaatacaagc tgcagagaga aaaggaaatc gaagaataag cgaagctttt
      661 caaaaagctg ttggcaattt tcgccaaatc tgccaaaatg tcaaaaattt cacttgcgtg
      721 gcgatatgaa aaaaccaatc ttcaaattaa aggcaatctg ctttttaatc gttttaattt
      781 ttctctctaa cgcttcggct caggaatccg cgctgacaac ggaagagggc aaaaggcttt
      841 tggctgaaat ccaggtcagc ctgcgggctg agaaacacga cgaagtgatt gaaaaatcaa
      901 ccagactgct ggcgatagcg ccaaaatccg ccgacgtgta catcctgcgc ggcacgtcgt
      961 ttctggtcaa aggcgaaaag gaaaaagcca tcaatgattt ttctcagggc ttgcagtttg
     1021 gactcagccc gcaaatggaa atagccgctc gcaaaatgcg cgcgattatg ctgttccaga
     1081 caaagaaata cgatgaggca ataccggatt acagcttcct gattcaaaaa gagtcgtccg
     1141 attttcgcgg ttatgtccag cgcggctggt gttttttcta caaaggaaat catcaggcgg
     1201 caatcgccga ttttgacagc tccttaaaat taagcccgca ggaaaaagga atccgccgct
     1261 accgcgcgat ttcgcattac cggctcggca attatgaaaa agccgtcgag gacgcgagcg
     1321 aggaaataaa aatcaatccc ggcgcgctcg ccgaagtgta taaaataaga gcggacgctt
     1381 accgcaagct cggcaaaatc gagctggcgg aaagcgacga gaaaaaattc gccgaaatga
     1441 gcgccaagcc ttcttccgcc ggagaggttc cgccgaagtc gaactttcag gcaaaattcg
     1501 aagaattaat cgaaaaatct cagaaccatt ttcgcaaagg cgagtgggac gcggcgattc
     1561 aggcttataa ccaaattatc gaactgctcg gcgaaagcga caaagctttg tatgccatct
     1621 atttttcgcg cggcagatgt ttttacgaaa aaggaaatct cgaccgcgcg cttgccgact
     1681 ataacgaagt tatcaaacgc cgaccggatt tagcgggcgg ttttactttt cgcggcgagg
     1741 tttatctgcg gagaaatgaa ctggattcgg ctttagatga ttttaccaaa gccataaaac
     1801 tggattcgaa cgatattctg gcatatcgcc gccgcgccga ggtttataac ctgaagaaag
     1861 attacgacaa cgccgtcaaa gactgcggcg acgtcatcag gctcgacccg aaatatcagt
     1921 ctgcctggta tctgagagcg gattcatatt ttgaaaaagg cgactttaaa gccgctttaa
     1981 aagatatatc ggaatatatc agattggacg atacgagcgc caaagcatat tccctgcgcg
     2041 cgaaaaccta ccggaaatta aacgatgaaa aagccgccgc ggcggacgaa aaaagagccg
     2101 ccgagctgaa caacggtcgt caaagtcatg aatgagaaaa tttcagccgt agtcattacc
     2161 gccagcgatt cgcgcgagga aaaagacgat gtatcgggcg tcacgctggt cggtctgctg
     2221 ctcgcggcgg gagcggaagt ggtggacaaa atcatcgttt cggacgacgc ggaaaaaatt
     2281 gtcgaaaccc tgaaagcgca agccgcgcgc gacgacgtta atttgatttt gacgaccggc
     2341 gggacgggac tcgcgccgcg cgacaatacg ccggatgcga cgcgcgccgt gattgaaaag
     2401 gaagtgcccg gaatggcgga ggcgatgcgg attggaactt tgcaaaaaac gccgatgtcg
     2461 atgctttcgc gcggcgtctg cggcacgcgc ggcaaatgcc tgattatcaa tcttcccggc
     2521 tcgccgaacg gcgttcgcga atgtttcgat attatcaaac ccgttcttca gcacgccgtc
     2581 aatctgctgg cgggaaaatc cgaacataaa aattaaatta aaatcggata cctgcaattt
     2641 gaatcaattc aatacaaccg gcttttttct taaccgtatt cgataagcgt tcacaaataa
     2701 aatttaagaa cgctcaatcg cgcctaaata attcttatca aataacttaa aacaatttcg
     2761 attctatttt gacgttcgtt tttctgctat atggaacgtg agttgctgag tgcaaaacat
     2821 cgctcagcat taatgtacat atattaaagg agaagataat ggcaaatccg gcggaaagcg
     2881 taaatacgga agcgcagcaa acccaggcgc aagccgataa cgcggcgata gtcgttccga
     2941 tcatacagga agaagtcacg tttgataaaa agattgtgga aaccggaaag gttcgcatct
     3001 ccaagcgagt cagccagcac gaacagctca tcgacgtgcc gtttatgcgc gaacaggtcg
     3061 atgtcgaacg cgttcccatt aatcaggttg tcgatgcggc gccgctggtg cgccaggaag
     3121 gcgacacgat gattattccg gtcgtcgagg aacagatttt ttatcaaaaa cgtctggtgc
     3181 tggtcgaaga attacgcgtt cgcaaacaaa ttatcgaaga acacaaaccg caacaggtca
     3241 ctttgctgaa ggaggaagtg gaaattaacc ggtctgtcga aaatgaaaac ttgagtcgtg
     3301 aaaaagggat ttaaaaattt aatcgccgaa aagggggaaa tttaaatgat acacacagtg
     3361 gtaggtttat ttgataacag aagcaacgcg ggcgcggcga cgcaggaatt aatccagaaa
     3421 gggtttctgc gcgaaaatat cgacgtttca aaccgccaga taagcgatgc cgcgacttcg
     3481 actcaaatcg ccgtcaccga aaccggcgtc agcgaaagca tcggcaattt tttcaattca
     3541 ttgtttggcg gagataaaaa aaccgcgaga aattatacga ccgccgccgg cgatgccgac
     3601 gcgattataa cggttcaggt cgattcgctg gaaagagcgc gcgaagccgc cgaaattctc
     3661 gaccgccacg gcgcgatcga cgtcgacggt cgctcgtcgc aacacgagca gaacctgcaa
     3721 caaaatcagc aaattttaac cgccgcgccc aatgcgccgc agacagcgca aaacgcagct
     3781 gatattcata acgcggctaa tattcagaac gaaacggcaa ttccggttat cgaagaacag
     3841 cttcaggtcg gcaaacaaat tgtccagcgc gaaggcgcgc gcgtcagaag ccgcattgtc
     3901 gaaaagccgg tcgaagcaaa tttgagattg cgcgaagaac acatcgtcgt caatcgccgt
     3961 tccgttaacc gcgatgtaac caatgcggat ttggcgaatt tccgcgaagg cgagctggtt
     4021 attaccgaac acgccgaaat tccggtggtc ggcaaacagg cgcgcgtggt cgaagaaatc
     4081 ctgatcggca aaaacgtgac cgagcacgtc gagacggttc acggaactat ccgccgtaat
     4141 gaagtagaag tcgaaaaagt taataccgat gttacggacg ccgacgacat caatacgcgc
     4201 ggcgcaacaa cttgaaattt attttagcga acgcgt
//
LOCUS       DASWNE010000250         2679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_8050, whole genome shotgun sequence.
ACCESSION   DASWNE010000250 DASWNE010000000
VERSION     DASWNE010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8050"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            284..628
                     /locus_tag="VGB68_05185"
     CDS             284..628
                     /locus_tag="VGB68_05185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0048656.1"
                     /translation="MAELNISERQAGDVTILDLNGKVTIGEGSVALRTTIRRLLGEDK
                     KKILLNLGGVGYIDSSGIGELVSSFTAVNKEDGSLKLLNLTQKIQDLLAITKLLTVFD
                     VFENEGEALSSF"
     gene            862..2652
                     /locus_tag="VGB68_05190"
     CDS             862..2652
                     /locus_tag="VGB68_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048657.1"
                     /translation="MAFITVFAQGVSGQQPTPTPKPAPVRQTQSESFDLTQYGVKIEP
                     DRRLIVVLASLEAAGLETPLTETGKAFREQLKNDLQATNPDLQQKLKTFLTQYKRRHP
                     NQTPAEISASFVSLAYTLTPVPELQDPPRSADLPAELLEVFDYAPLVREFYRRSGISA
                     RLEDYLKEYQKSGDAMRGSATVMVRDLLDYLHTRPQLAYIDRVKVETKDAKGKSKLEK
                     TEARQRERRFFIVPDLLAPAGTINFRNVGDDYYVIVPPNTNLISSEARRAFLQFTIDP
                     FILQNGREISAFREGIKTLLDERRKINPDISPDIYLAVMRSMVAAVDARQVEFVKLQA
                     ATADARRRIDLAQGDDAKRAVSAELARFKTQLADETALELSEAYERGAVLSFYFAEQL
                     KGTEDSGFDVAGSFRDFVLSLDPSKETNRLAQFADARKRATTAREERRRKASTQQITT
                     AELTPREKVLKQKLEQIELVIKAKDYEKADEQLGELLKENEGDPRILYARGRVASLSA
                     ELAFDEGLRDERLGRAAAHYRNAITHSNSETDLILKQRAHVALARILEFSEQSEAALA
                     EYEAAVKIGDIDAQAYNEAMAAKQRLTKKP"
BASE COUNT          709 a          665 c          701 g          604 t
ORIGIN      
        1 ttgtttatgc ggaactttat ggaagaagtc gaatggtttc aacatccgca gggcggaacg
       61 gtggtcaaaa tgtgtaaatt gcgcggaacg tgaaaaagtg aaagagtgga aatgtgaaaa
      121 agcgaaaaag cgaatcgaat attaatcaat gagacttttt ctatcaatcg acaaaacttt
      181 ttcactcttt cacatttcca ctttttcact ttgaagtttt gtgttttgaa gattttcgac
      241 taaaatctgc tatcaaattc aaatttataa cgaggagata aaaatggctg aacttaatat
      301 ttctgaacgt caggcgggcg acgtgacgat tctcgacctg aacggcaagg taactatcgg
      361 cgaaggcagc gtcgcgctgc gcacgacgat tcgccgcctg ctgggcgaag acaaaaagaa
      421 aattctttta aatctgggcg gcgtcggcta catcgattcg agcggcatcg gcgagctggt
      481 ttcgagcttt accgccgtca ataaagaaga cggcagcctg aagcttttga atctgacgca
      541 aaaaattcag gaccttctgg cgattaccaa gctgctgacc gttttcgatg tttttgaaaa
      601 cgaaggcgaa gcgctgagca gcttttaaac cgaagtttta atcgaaccgg aaatttccaa
      661 gccctcggcg cttacgaggg cttgggcttt tctttttcta aataaggcat ctaaaaaatc
      721 ccgcaaagca tctttattca aagcaaaggc acaaatcaaa cagagaaatt tccagttata
      781 ttcctccttc attctttgct tgatttgcgt cctttgcgtt tcctttaatt tccgaaaagc
      841 acaatgagaa aagttttatt tttggctttc ataacagttt ttgcgcaggg cgtttcgggg
      901 cagcagccga cgccgacgcc caaaccggct cccgtacggc aaactcaatc ggagtctttt
      961 gatttgaccc agtacggcgt gaaaatcgag cccgaccgcc gtttgattgt cgtgctcgcg
     1021 tcgctcgaag ccgccgggct ggaaacgcct ctgaccgaaa cgggaaaagc tttccgcgag
     1081 cagttaaaaa acgatctgca ggcgaccaat ccggacttgc agcaaaagct gaaaactttt
     1141 ctgacgcagt ataaacgccg tcacccgaac cagactccgg cggaaatcag cgcctctttc
     1201 gtctctttgg cttacacttt gacgcccgtt cccgaattgc aagacccgcc gcgcagcgcg
     1261 gatttgcccg ccgaattgct cgaagttttc gattacgcgc cccttgtgcg cgaattttat
     1321 cgacgctcgg gaatcagcgc gaggcttgaa gattatctga aggaatatca aaaatcgggc
     1381 gacgcgatgc gcggttcggc aaccgtcatg gtgcgcgatt tgctcgatta tctgcacacc
     1441 agaccgcagc ttgcttacat cgaccgcgta aaagtcgaaa cgaaagacgc caaaggcaaa
     1501 agcaagctgg aaaagaccga agcgcgccag cgcgagcgac gctttttcat cgtgccggat
     1561 ttgctggctc ccgccggaac tattaatttt cgcaacgtcg gcgacgatta ttacgtaatc
     1621 gttccgccga acacgaatct gatttcttcg gaagcgcggc gcgcctttct gcaatttacg
     1681 atcgaccctt tcattctgca aaacggcagg gaaatttccg cgtttcgcga aggaataaaa
     1741 actctgcttg acgagcgccg caagattaat ccggacattt caccggacat ttatctcgcc
     1801 gttatgcgct cgatggtcgc ggcggtcgat gccagacagg tcgagtttgt gaaattacag
     1861 gcggcgacgg cggacgcccg gcggcggatt gatttagcgc agggcgacga cgccaaacga
     1921 gcggtttcgg cggagctcgc gagatttaaa acacagttag ccgacgaaac ggcgcttgag
     1981 ctttccgaag cctacgagcg cggcgcggtt ctgtcgtttt attttgccga acagctgaaa
     2041 ggcacggaag attccggctt tgacgttgcc ggctcgttcc gcgattttgt tttgtcgctc
     2101 gacccttcga aagaaacaaa tcggctcgcg caatttgccg acgctcgcaa acgcgcgacg
     2161 acggcacgcg aagagcgccg gagaaaggct tcgacgcagc agattactac ggcggaattg
     2221 acgccgcggg aaaaagtttt aaagcagaag ctcgaacaaa tcgagctggt catcaaggcg
     2281 aaagattacg agaaagccga cgagcagctc ggcgaattgt tgaaggaaaa cgagggcgac
     2341 ccgcggattt tatacgcgcg cggtcgcgtg gcgagccttt cggctgagct ggcttttgac
     2401 gaaggcttgc gcgacgagcg tttaggacgc gccgccgctc attatcgcaa cgcgattact
     2461 cattcaaaca gcgaaaccga cttgattttg aagcagcgag cgcacgtcgc gctcgccaga
     2521 attctggaat tcagcgaaca gtccgaagcc gcattggcgg aatacgaagc cgccgtcaaa
     2581 atcggcgata tcgacgccca ggcttataac gaagcaatgg cggcgaaaca aagattgacg
     2641 aaaaaaccgt aaaagaaggc gaaagtaaga aggaaaagg
//
LOCUS       DASWNE010000251         3053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_502041, whole genome shotgun sequence.
ACCESSION   DASWNE010000251 DASWNE010000000
VERSION     DASWNE010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502041"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /gene="secF"
                     /locus_tag="VGB68_05195"
     CDS             <1..722
                     /gene="secF"
                     /locus_tag="VGB68_05195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEY0048658.1"
                     /translation="GRATVKKALDNFGKEAAATATLNDDQSAGYKIVGTESVGPVAGD
                     QLRNQAVAATLLGLLGILLYIAYRYDWTYAAGAVIAVFHDVLVTLAFFSVFQWEINLT
                     VIAALLTLVGFSVNDSIVIFDRIRENLTLHRDKSLYQLTNDSINQTMSRTIVTNGLVF
                     LSVLALVIFGGEVLRGFSLALFVGSITGTYSTIAIASPIAIWWQDKLGAAKTKQPEIR
                     KDANTLATASRRSVARKTATR"
     gene            1217..1966
                     /locus_tag="VGB68_05200"
     CDS             1217..1966
                     /locus_tag="VGB68_05200"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY0048659.1"
                     /translation="MTTLFAGGIVYSLFAKDLGTSDEGLELSTLVAPPLPEEAPPPPP
                     EPEQKPEKQTTTEKIDVTTVKEAYSTIENTKEPPKETTGQKQVIGLNPNEKFRIGENV
                     GRATNPQAGPDRSGEGGGGITPTTAPPTTEEEPPPPPPKPTPTPKPEPTKAPVPKIIS
                     KGVVNGSAISLPKPPYPPAAKAVRAAGTVSVQVTIDENGNVISASAAGGHPLLQQAAV
                     GAARQAKFRPTLLSGTPVKVTGIITYNFVAQ"
     gene            2089..2820
                     /locus_tag="VGB68_05205"
     CDS             2089..2820
                     /locus_tag="VGB68_05205"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY0048660.1"
                     /translation="MTFLASFFLMFAEGGTKISFGLYDIAKSLTIPGWAVVIILLLMS
                     IYIIAIGIERWMTYNAAKQQSRQFAPKVAQALKNNNVDEAINISDKHKNSHLAMVVSS
                     GLKEFSAHQGNSDISGEEMEASKRALQRAIAVKQAELKRGLSGLATVGSTAPFVGLFG
                     TVVGIIGAFVALATEQNAGIGAVGGAIAEALVATAFGIAVAVPAVWLFNYFTNKVSSF
                     TVEMENSASELIDYFIKQRTKQLRG"
BASE COUNT          775 a          727 c          814 g          737 t
ORIGIN      
        1 cgggacgcgc gaccgttaaa aaagccctcg ataacttcgg caaagaagcg gcggcaacgg
       61 caactctgaa cgatgaccaa agcgccggct ataaaatcgt cggaaccgaa tcggtcggtc
      121 cggtcgccgg agatcaattg cgtaatcagg cggtcgcggc gacgctttta ggtcttctgg
      181 gaattttgct gtacattgcc tatcgctatg actggacgta tgcggcgggc gcggttatcg
      241 ccgttttcca cgatgttctg gtgacgctcg cgttcttttc ggtctttcag tgggaaatca
      301 acctgacggt gattgcggcg cttttaacgc tggtcggttt ttcggttaac gactcgattg
      361 taatctttga ccgtattcgc gaaaatctga cgctgcaccg cgacaaatcg ctttatcaat
      421 tgacgaacga ttcgattaac cagacgatgt cgcgcacgat tgtgaccaac ggcttggttt
      481 ttctgtcagt gctggcgctg gttatctttg gcggcgaagt gctgcgcgga ttttcgctgg
      541 cgctttttgt cggctcgatt accggaacgt attcgactat cgctattgcc agcccgattg
      601 ccatctggtg gcaggataag ctcggcgcgg cgaaaaccaa acagcccgaa atacgcaagg
      661 acgctaatac tctggcaacc gcttcgcgac gctcggtcgc cagaaaaacc gcgacccgat
      721 aaaacgtgag agcggctcgg ctaagtcgct cttgctgcaa attttaattt tctataaatt
      781 ttctttgcga tttcggaaaa aagcataagg aaataagaaa ttttccttga ctttctgttt
      841 tcaaaatctt agacttcaaa tcgttgctgg tcaaaaatta tttatacgtt tctgcttttt
      901 tccgattcgt ttatagagga tgaaaacgga gttggcggaa ctttgcatat ccgactggca
      961 aagtccgctt acccgcctca ttaaaagatt taacgcgtca gaaaagcagt tttggttgtt
     1021 gtggaaaacc gatgacaaca aaggttaaat tttattcatc agagcagtta taaagaaaat
     1081 ttcgtagccc gaccccgatt ggcaccggat aagagggagg taaataaaaa ctatgttaga
     1141 tcaattagtc gaatcgaaaa gtcactcagg agaaaatact aagcgaggcg gatttctctt
     1201 aacgacattc gttattgtga ctactttatt tgcgggcggt atcgtttata gtcttttcgc
     1261 aaaagacctc ggcacgagcg atgaagggct ggagctttcg actctggtgg ctccgccgct
     1321 gccggaagaa gcgccgccgc cgccgccgga accggaacag aagccggaaa aacagacgac
     1381 gaccgaaaag attgacgtga cgaccgtcaa agaggcgtac agcactatcg aaaatactaa
     1441 agagccgcct aaagagacga ctggacagaa acaagtcatc gggctgaacc cgaacgaaaa
     1501 gtttcggata ggcgagaatg tcggtcgcgc taccaatccg caagccggac ccgatagaag
     1561 cggtgagggc ggaggcggaa ttacgcctac tacggcgccg ccgaccactg aggaagagcc
     1621 gccgccgccg ccgccgaagc cgacgccgac gcctaagccg gagccgacta aagcgcccgt
     1681 accgaaaatt atttccaagg gcgtcgtcaa cggcagcgcc atcagtctgc cgaaaccgcc
     1741 gtatccgccg gcagccaaag cggtgcgcgc cgccggaaca gtttcggtgc aggtgacgat
     1801 tgacgaaaac gggaacgtta tttcggcaag cgccgcgggc ggtcacccgc tgctgcagca
     1861 agcggctgtc ggcgcggcac gtcaggcgaa attcagaccg acattacttt cgggcacacc
     1921 ggtgaaagtt acgggaataa tcacctataa ctttgtggct cagtagttcc gggaaagttt
     1981 tcgagttccg ctttcaaagg ctttagagcc gaacgatttg gcgtcctgac ggggcgggac
     2041 tcaaaactct gtaataataa tttttttgat attctttgga ggataaccat gacattttta
     2101 gcaagctttt tcttaatgtt tgccgaggga ggcaccaaaa tttcttttgg tctttacgat
     2161 attgcaaaaa gtttaacgat tcccggctgg gcggttgtta tcattctttt gttaatgtcg
     2221 atttacatta tcgccatcgg catcgaacgc tggatgacgt ataatgcggc gaaacagcaa
     2281 tcgcgccaat ttgcgccgaa agtcgctcag gctttgaaaa acaacaatgt cgacgaagcg
     2341 attaacatca gcgataaaca caaaaattcg catctggcga tggtcgtctc gtcggggtta
     2401 aaagaattta gcgcgcacca gggcaacagc gatatatcgg gcgaggaaat ggaagcctcg
     2461 aaacgcgctt tgcagcgtgc cattgccgtt aaacaagccg aattgaagcg cgggctttcc
     2521 ggtctggcga cggtcggttc gacggctccg ttcgtcggat tgttcggaac ggtcgtcggt
     2581 atcatcggcg cgttcgtcgc tttggcaacc gaacagaatg ccggtatcgg cgcggtcggc
     2641 ggcgccatcg cggaagcgct cgtggcgacg gctttcggta tcgccgtggc ggttccggcg
     2701 gtgtggctgt ttaactattt tacgaacaag gtttcgagtt ttactgtcga aatggaaaac
     2761 tccgcttctg aattgatcga ttactttatc aaacagcgca ccaaacaatt gcgcggataa
     2821 tctaatcgaa aaattgaagg tagttatttc tacaacaaaa ctttaagcat tatctaagga
     2881 gaaatgaact atgtcgatgt cagttggcgg agatagtgaa tataactctg agattaacgt
     2941 gactccgatg gtcgatatta tgctggtgct gctgatcatc tttatggtcg tgacgccgct
     3001 gctgcaacag ggagtttccg ttaatcttcc gcgtgatatg aacgcagccg aag
//
LOCUS       DASWNE010000252        15081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_677925, whole genome shotgun sequence.
ACCESSION   DASWNE010000252 DASWNE010000000
VERSION     DASWNE010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 15081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15081
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677925"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..462)
                     /gene="ssrA"
                     /locus_tag="VGB68_05210"
     tmRNA           complement(91..462)
                     /gene="ssrA"
                     /locus_tag="VGB68_05210"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            861..3980
                     /locus_tag="VGB68_05215"
     CDS             861..3980
                     /locus_tag="VGB68_05215"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEY0048661.1"
                     /translation="MNSVLRLPYWAKAALGLLLFATIIFAVSLQFRRAGKPPIHQPKT
                     AGEFEENAEARNEWFMRERTFPTGEIPADARRNAWASRPVDARLSETSAAPPVWQSIG
                     PRATESDIDNWGLTSGRINAIAVSPSNPQLILVGAATGGIWRSIDGGANFSPTTDNQV
                     DLSVGSIAFAPSDNSIVYAGLGDKSSNYFGSGVLKSTDGGQSWTRVSNATLPSPGRVS
                     QIEVDSANPNRVYAAQYTNRQGNGSFASGFYYSIDGGVNWTKTIAGLARDLIKHPTQP
                     NTLYLGMARVDGTTPTTGGVFKSDNGGQSWTRIYTAPFAATSNIKIAVTPAAPQSLYV
                     LSGSTAPAGARLETSANEGGMWTNRGSNFDIGQLSYNFYLFVHPTNPSIIFVGTRDLW
                     RSVNGGTNFTNITNNFSITGAYNPFNSRAHPDQHHFYISSSDPNIIYLANDGGFYKST
                     DGAGSFQSLNASLNLTMFTSLDLHPTDASRTYGGTQDNGNQRRRGGELWREFISGDGG
                     QIVVDPIDPGIVYATYVRHVIDRWSSNTDVYDLPIGNSAVFNNDPVAFYPPFVGNDVN
                     SNLYFGTNRLYISANRGLTWTPPAGALDLTFGGGDVLSAIAVAKSDTNVIYTGSSFGR
                     AMVSTAAGGGFTQITNGLPARFIKSIVVSPSNPSVAYLTVSGFDSGHVFKTINAGANW
                     TDISGNLPNIPTNTLVIDPRDPNTLYVGTDIGVFRSLTDGGTWETFNNGMPPVIVSEL
                     DINAGGLLQAGTYGRGAYQIDTAAAAPRAVADFDGDGKTDVSVFRPADGTWYLLRSQA
                     GFTAAQFGANGDIPAAGDYDGDGKTDIAVYRPNGGFWFIVNSSNNTFRGQQFGISSDK
                     PVANDFDGDGKTDIAVYRGGAWYSIYSSNSNLLATNFGIATDLPAPADFNGDGRAELA
                     VYRDGTWYTLNLANNEFKGVQFGIAGDKPVVGDYDADGKDDYAVYRPTDGTWYVLRST
                     SGFFGQQFGIATDIPAAGDYDGDGKSYLAVYRPADGAWYIINSGNSSLRVANFGVNGD
                     IPIPSRQNP"
     gene            complement(4108..5304)
                     /locus_tag="VGB68_05220"
     CDS             complement(4108..5304)
                     /locus_tag="VGB68_05220"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0048662.1"
                     /translation="MSKSRMSWAEMLDVLTDSDRRRNTILEKPSEIERMALFLLRREG
                     FSAQLAPQTQKNYDLELFKPGERLAVSVRNHRAKAHLGQIEKFLDFLDKENSKTFTGG
                     IFISTSGFTPSVYAYLREEKITRLRLAILRGNHLIWDCEKIDSAEERERTTYIGVFTC
                     KGGVGKTTIAAHLAGAFALNGYDVALLDLDRQQNLKKLLGEGVYLPATKGQIGSVISV
                     LSHEDWDEAAYKDTKIVVCDCNPEFDANPTEFIRNFDYCLIPTTLNPLGINKNADVIK
                     RTFTEIRRVNQKAHLHVLINNLHADEENRNALLNQVLKTQFKQFTERDSRCHYIEPAE
                     CAIRFSKQLLYWGFHIFENTRPQLAFRSIGGYSYPRMDFLKLVDYMEMQTQFQKAKAR
                     FFTAGS"
     gene            complement(5379..5837)
                     /gene="bcp"
                     /locus_tag="VGB68_05225"
     CDS             complement(5379..5837)
                     /gene="bcp"
                     /locus_tag="VGB68_05225"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006960.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEY0048663.1"
                     /translation="MLNEGDTAPDFTTTDQSGKQVKLSDFRGQRVVLYFYPKDDTPGC
                     TKQACSLRDSYDVFTEKGIKVLGVSNDDEASHRKFISKFSLPFDLLADTEKEIVSAYG
                     VYGEKNMYGKKYMGINRTTFLIDEEGKIKKTFKKVNVDEHADEVLKAFNN"
     gene            6025..6558
                     /locus_tag="VGB68_05230"
     CDS             6025..6558
                     /locus_tag="VGB68_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048664.1"
                     /translation="MKTLSEKQALLKLGKLKNDFQSFIKENYERRAKDCLTCPAQGEC
                     CTDAHFVNVHITRLEAAAAREALRKFDEAKQKEIYKRAAEAVEKYDLKDSGDTFAQTF
                     ACPLFEKGVGCLIHKEGKPAPCIQHACYERRADLPPDELQSAAEQKIERLDARAYRQN
                     TNWLPLPLWLSRINPFD"
     gene            complement(6555..6968)
                     /locus_tag="VGB68_05235"
     CDS             complement(6555..6968)
                     /locus_tag="VGB68_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048665.1"
                     /translation="MLRIILGAIIGFIVWSILWVGSDALFSAISPGWYGRHHAELEAA
                     VNNKTAFMSDSTILIIGLVRSVIFSIVAGFIAALIAGENSKSTLACGVLLLLFGIFIQ
                     SIFWNYAPLWYHILFLLLLIPMTILGGKLKKARLN"
     gene            complement(7296..8327)
                     /locus_tag="VGB68_05240"
     CDS             complement(7296..8327)
                     /locus_tag="VGB68_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048666.1"
                     /translation="MTKPKINPLSRIPRQQRLVMAIRGGAGVGKSHFIRSMSEAGLGK
                     LCIFDTERKARLLRGVGVQFDALEIENTDELPEFIDWAINGEGREQNYGCFALDSWAA
                     YFGAKHSDMIEAVREKAGDPLAQPSAEELAADQMILQGVLRKLCIESGKSIVIADQIP
                     AKGKEAKEDNEIGRVLPMTASGLEYFVDVMVEVSLQERDGEIVRVFQVVKSNSPAFEV
                     GYEFVGNVTFKDFLDHMRGEHALELAFEKPAPSKVPEFIEERQPLSLVPNTLTIDELV
                     AKAEANGFKKADIVTGAKIYHNQPNIYHLTTEQIADLDRRMTARIEKTKNQDVAASEK
                     TNAKRVKVA"
     gene            complement(8691..9329)
                     /locus_tag="VGB68_05245"
     CDS             complement(8691..9329)
                     /locus_tag="VGB68_05245"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HEY0048667.1"
                     /translation="MTGKIKIAHVENDFSVSELDSNYWEKSQTVAIEKYWSGGNAPRG
                     RHAKARLLWSETALYVRFEANQAEPLIISETPNLKTKTRGLWDRDVCEIFVAPDKSEP
                     RRYFEFEIAPTGEWIDLAIHQLAERRETDFEYDSEMLAAARIEKDKVLMAMKIEFKAF
                     GKTPKANEVWRGNLFRCVGAGETRGYLAWQPTETPQPAFHVPEAFGEFEFVK"
     gene            complement(9360..9671)
                     /locus_tag="VGB68_05250"
     CDS             complement(9360..9671)
                     /locus_tag="VGB68_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048668.1"
                     /translation="MEPANILKIESLPDDWRDKDEIILHACFQLLKDFVEREKEIFEQ
                     IDWKHDEETEKAKAEIDFLYNWWLKRFHDEEDFDKIKRRYEEDNQMLKRLIDVRKYLW
                     S"
     gene            complement(9679..11436)
                     /locus_tag="VGB68_05255"
     CDS             complement(9679..11436)
                     /locus_tag="VGB68_05255"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEY0048669.1"
                     /translation="MQKDSAMEENKQALMLKENSIEAIPAADGNHAPAGNRALPASKQ
                     ISNEQLIAEEKSLLAQEESVEKNYEGWRGYLRLFHVSRVIGMLSLYLYLDQYDLHHAQ
                     HEKHAEARFETAKRLTWLAVLGEYLYKIRLFFFHRFMLPVRRFFIGGETNRELNQEKQ
                     AVWLKENLINLGPTFIKIGQSLGTRADLLPLPFVKSLGELQDSVPPFPNEIAFARIEK
                     ELGKKINEVYEEFDVEPIAAASLGQVYRAKLFTGEEVAVKVQRPNLAGIIKGDIEILR
                     KVTKFAERFPSLNENADWAGMLREFDETIHEEMDYAAEGRNAERFQESFKDWTNVHVP
                     KIYWRATSEKVLTMEFIHGTKVVALDELKARNVSPEKVNRLLIRTYLKQLLEDGFFHA
                     DPHPGNLLVMPDGRLAFFDFGMVGRITPQLQSKMIDAFFHVVAKDPAGIAQDLIDLDF
                     MKPGSNPDIVRPVVEKMFQFHLNLKLKEVNFKELTYDLADVMYDYPFRLPSNFTYIMR
                     ALMTLEGIGIITDPEFNFFETAKPYAKEFMLRREGKDLRKLLTDKILGRDTEDGKIDW
                     DRSWKLAKMAAKTIWNSRK"
     gene            complement(11645..12598)
                     /locus_tag="VGB68_05260"
     CDS             complement(11645..12598)
                     /locus_tag="VGB68_05260"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004159.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEY0048670.1"
                     /translation="MKIFHGTENANISRPTVLTLGVFDGLHLGHQRIMQTVVERARAA
                     SAVPTAITFDPHPRAVLYPESAPPLLQTLDQRLANFEVLGIEQAIVIRFNREFAAQDA
                     REFLQNVVHERLQAKEVYLGKGFAFGKNRAGNIDLLRQISTGLGFHADEVPEVRLRGA
                     RISSSKIRALLAVGKINLTRRMLGRPYGIEGQIIHGAERGRTIGFPTANLKPRNRVVP
                     KFGVYATATLIAGEWRRSITNVGVRPTFEKEIEPSIETYVFDYDGDLYGNVLRVRFLH
                     RIRDEKKFGGIDELKTQIEKDTKRALLYFRRQGVNNSLDII"
     gene            complement(12618..13070)
                     /locus_tag="VGB68_05265"
     CDS             complement(12618..13070)
                     /locus_tag="VGB68_05265"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0048671.1"
                     /translation="MAADYQSAGENRYDAGVKDFDAYLQRLAMFASGVNLPAERVPSS
                     EFYLLGDGRLIGRAALRRSLNAELEIIGGHIGYDIRPSERGRGFGTLILRLVLEKARE
                     IGLKKVLLTCDADNVASARVIENNGGRLDKQIIYEKTGKLISQYWIEL"
     gene            complement(13141..13602)
                     /locus_tag="VGB68_05270"
     CDS             complement(13141..13602)
                     /locus_tag="VGB68_05270"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0048672.1"
                     /translation="MKIKFRRATREDLPEIVRMLSDDFLGRARERYENPLPESYIKAF
                     EEIEADKNNELIVAETGGEILGTLQITFTPSISFQGGKRATIESVRVDEKYRRRGIGK
                     KLMLWAIKRAREENCFAVQLTTNAERADAHRFYENLGFKGSHLGMKLYLKK"
     gene            complement(13605..14366)
                     /locus_tag="VGB68_05275"
     CDS             complement(13605..14366)
                     /locus_tag="VGB68_05275"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0048673.1"
                     /translation="MKLTFLGTGTSTGVPSIGCECETCLSDNPKDKRLRVSVTIEHAG
                     KKILVDTSIDFRQQALRARINYLDAVLITHCHVDHVFGLDDIRPLNFRYGAMGIYANE
                     IAWKDLRRIFQYIFQPTHFGGGLPQLIPHTVVKNSPFCIGEDLLITPLEVIHGKLPVV
                     AYRFNDFAYATDLKFIPEDAMRGLTDLDVLVLDCVRIKPHSTHLGLSEALEYIEKLKP
                     KRAFLTHLNHDILHERDSRLLPDNVQFAYDGLIVE"
     gene            complement(14461..14841)
                     /locus_tag="VGB68_05280"
     CDS             complement(14461..14841)
                     /locus_tag="VGB68_05280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEY0048674.1"
                     /translation="MSTFKRFEEIQAWQKAREITKKIYSMSNKDNFSKDFSLRDQMKR
                     ASVSIMANIAEGHGRRTTTEFANFLNIARGSAIELQSHLYVALDLNYIEQTEFREAYE
                     MLDEVSRMTLSLAQYLRKKVDRVD"
BASE COUNT         3589 a         3938 c         3656 g         3898 t
ORIGIN      
        1 aaaacggcaa atagccgctc gctgaaaatc acctcttagc cctattttat cggtatttat
       61 taggttttag aaaaaaacag aaaaaaaatt tggtggaggc ggcgggaatc gaacccgcat
      121 ccaaaggttg cgaccagtag actctacacg tttattcaat ttgcttaaag tttcgcctgc
      181 aaaaagtcag caaactgaca aaaccttttt acagactagc ctgactaaca tttcgctcgc
      241 gtccgcaggc ggtcgacgcg cgctagcttg aactaagtta tgcctcaatc ggtcgcttca
      301 agctgacttc cgctgagaca cttgtggtta gtttttaact aagcagcaag agctaattct
      361 tgattagcat ttgtgttttg cttggtgttt taacgagcta acaagcaagc ccgacgtgca
      421 tccaaagatc tatacaagcc cctgtcgaag cctgtcgccc ccaaaagttc gggaaagcta
      481 ttctacaaca tatttgatga aaaactaaac cggcggtttg cccgcaacct gcgcggcgat
      541 aaattcccgc gcggcgaaaa ccggacgccg tcggaaactg acgatttaat ttatcgtttt
      601 cgtcggacgc cgttcgattg caggacgaaa acgtcgccgc gtcattttgt tttcggcgct
      661 gcaaccagtt ttcgaccttg ttcatcaaat gcattgattt cacatcgttt caatcaagat
      721 tggttttata ttcttgtatc tgacttttat ctttttattt aagttagtat aaacagttat
      781 ctttcaagca gggcgtttta cctttcaagc agggcgtttt acattcccac actattttgg
      841 acaattttca ggagatttgt atgaacagcg ttttacgtct gccgtattgg gcaaaagccg
      901 cgctcggtct gcttttattc gcaactatta ttttcgccgt cagcctgcaa ttcaggcgcg
      961 ccggaaagcc tccgattcat cagccgaaaa ctgccggcga atttgaagaa aacgccgaag
     1021 cgcgcaatga atggtttatg cgcgaaagaa cgtttccgac cggcgaaatt cccgccgatg
     1081 cgcggcgcaa tgcctgggca tcgcgccccg tcgacgcgcg tttgagcgaa acgtcggctg
     1141 cgccgcccgt ctggcaatcg attggaccgc gagccaccga atccgacatc gacaactggg
     1201 gattgaccag cgggcgcatc aacgccatcg ccgtttcgcc ttcgaacccg cagcttattt
     1261 tagtcggcgc ggcgacgggc ggaatctggc gctcgattga cggcggcgcg aatttttcgc
     1321 cgacgacgga caatcaggtg gatttatcgg tcggctcgat tgcctttgcg ccgagcgaca
     1381 actcaatcgt ttacgccgga ttgggcgata aatccagcaa ttatttcggc agcggcgttt
     1441 tgaaatcgac cgacggcggg cagagctgga cgcgggtcag caatgcgacg ctgccgtcgc
     1501 cgggcagagt ctcgcaaatc gaggtcgatt ccgccaaccc gaatcgcgtt tatgcggcgc
     1561 agtataccaa ccggcaggga aatggcagct ttgccagcgg attttattat tcgattgacg
     1621 gcggcgtcaa ctggacaaaa accatcgccg gtttagccag agatttgatt aaacacccga
     1681 cgcagccgaa cacgctttat ctgggaatgg cgcgcgtcga cggcacgacc ccgacgaccg
     1741 gcggcgtctt taaatcggat aacggcgggc aaagctggac gcgcatttat accgcgcctt
     1801 tcgccgccac ttcaaacatc aaaatcgcgg tcacgcccgc cgcgccgcaa tcgctttacg
     1861 tgttgtcggg ctcgactgct ccggcgggcg cgcgtttgga aaccagcgcc aacgaaggcg
     1921 gaatgtggac gaatcggggc tcgaatttcg acatcggaca actcagctat aacttttatc
     1981 tgttcgttca tccgactaac ccgagcataa ttttcgtcgg cacgcgcgac ctctggcgca
     2041 gcgtcaacgg cggcacgaat tttacgaaca tcaccaacaa tttttcaatt accggcgctt
     2101 acaacccgtt caattccaga gcgcaccccg accagcatca tttttatatt tcgtcgtccg
     2161 acccgaatat tatttatctg gcaaacgacg gcggatttta taaatcgact gacggcgcgg
     2221 gcagctttca atcgctcaat gcgagcctga atctgacgat gtttaccagc ctggatttgc
     2281 acccgaccga cgcgtcgcgc acttacggcg gcacgcagga taacggcaat caaaggcggc
     2341 gcggcggcga gctgtggaga gaatttatca gcggcgacgg cgggcaaatc gtggtcgacc
     2401 cgattgaccc gggcatcgtc tacgccacct acgtcaggca cgttatcgac cgctggagca
     2461 gcaacaccga cgtttacgat ttgccaatcg gaaacagcgc ggttttcaac aacgacccgg
     2521 tcgcgtttta cccgccgttt gtcggcaacg acgttaattc caacctttat ttcggaacaa
     2581 accgacttta catcagcgcc aatcgcggct tgacctggac gccgcccgcc ggcgctctgg
     2641 atttgacctt cggcggcggc gacgtattgt cggcaatcgc cgttgccaaa tccgatacaa
     2701 acgtgattta caccggctct tcattcggca gagcgatggt cagcaccgcc gctggcggcg
     2761 gctttacgca aatcaccaac ggcttgcccg cccgttttat taaaagcatc gtcgtcagcc
     2821 cgtctaatcc gagcgtcgct tacctgacgg tttcgggttt cgattccggt cacgttttca
     2881 aaacgattaa tgccggagcc aactggacgg acatcagcgg caatctgccc aatattccga
     2941 ccaatacgct cgtcatcgac ccgcgcgacc cgaacacgct ctatgtcgga acggacatcg
     3001 gcgtctttcg ctcgctcacg gatggcggca cctgggaaac cttcaataac ggtatgccgc
     3061 cggtgattgt ctccgagctt gacatcaacg ccggcggctt gctgcaagcc ggaacttacg
     3121 ggcgcggcgc ataccagatt gataccgccg ctgcggctcc gagagcggtg gctgattttg
     3181 acggcgacgg caaaacggac gtttcggttt tccgacctgc ggacggaacg tggtatctgc
     3241 tcagaagcca agccggcttc accgcggcgc aattcggcgc taacggcgac attccggcgg
     3301 cgggcgatta tgacggcgac ggcaaaaccg atattgccgt gtatcgaccg aatggcggct
     3361 tctggtttat tgtgaacagc tcaaacaata ctttccgcgg gcagcagttc ggaatttcca
     3421 gcgataaacc cgtcgccaac gatttcgacg gcgacggcaa aaccgacatc gccgtttatc
     3481 gcggcggcgc gtggtattcg atatacagtt cgaattccaa cctgctggcg accaatttcg
     3541 gaatcgcgac ggaccttccc gcacccgcgg atttcaacgg tgacggtcgc gccgagctgg
     3601 cggtttatcg cgacggcacg tggtacactt tgaatctggc aaacaatgaa ttcaaaggcg
     3661 tgcagttcgg catcgcgggc gataagccgg tcgtcggcga ttacgacgcc gacggcaaag
     3721 acgattacgc cgtgtatcgt ccgacggacg gaacgtggta tgttctgcgc agcaccagcg
     3781 gatttttcgg acagcaattc ggcatcgcaa cagatattcc ggcggcaggc gattacgacg
     3841 gcgacggcaa atcttatttg gcggtttatc gtccggcgga cggcgcgtgg tatatcatca
     3901 acagcggaaa ttcgagcctg cgcgtcgcaa acttcggcgt caacggcgat attccgattc
     3961 cttcaaggca gaatccgtaa gtctgccggg aacaattaaa aggacggcgg gcgattaaga
     4021 taaatcataa atcttatatc gcccgccgtc cttttaattg tcctttcgga ataaaattca
     4081 aaactaacgg cgtaatattt gtgcttttta gcttccggcg gtgaaaaacc tggctttcgc
     4141 tttctgaaac tgcgtctgca tttccatata atcgaccagc ttcagaaaat ccatccgcgg
     4201 gtacgaataa ccgccgatgg agcgaaaagc gagttgcggg cgcgtgtttt caaaaatgtg
     4261 aaagccccag tagagaagct gtttgctgaa acgaatggcg cactccgccg gttcgatata
     4321 atgacagcgc gaatcgcgct cggtaaactg cttgaactgc gtttttaaaa cctgattcag
     4381 gagcgcgttg cgattttcct cgtccgcgtg cagattgttt atcaaaacgt gcagatgcgc
     4441 tttttgattg acgcggcgaa tctccgtaaa agttcgcttg ataacgtcgg cgtttttgtt
     4501 gatgccgagc gggttgagcg tcgtcggtat caaacaataa tcgaaattgc gtataaattc
     4561 ggtcgggttg gcgtcaaatt cgggattgca gtcgcagacg acgattttcg tgtctttata
     4621 agccgcctcg tcccaatcct cgtggctcaa aaccgaaatc accgagccga tttgcccttt
     4681 cgtcgcgggc agatagacgc cttcgccgag cagctttttc aagttttgct ggcggtctaa
     4741 atcgagcagc gcgacatcgt aaccgtttaa ggcaaacgcg cccgccagat gcgccgcaat
     4801 cgtcgtttta ccgacgccgc ccttgcaggt gaaaacgccg atataggtcg ttctctcgcg
     4861 ttcttcggcg ctgtcgattt tctcgcaatc ccaaatcaga tggttgccgc gcagaatcgc
     4921 cagcctcaag cgcgtgattt tttcctcgcg caggtaagcg taaaccgaag gcgtaaaacc
     4981 gctggtcgag ataaaaatcc cgcccgtgaa ggtcttcgag ttttccttgt ccagaaaatc
     5041 gagaaacttt tcgatttgtc cgagatgagc cttcgcgcgg tgattgcgaa ccgaaacggc
     5101 gagcctttcg ccgggcttga aaagctcgag gtcgtagttt ttctgcgtct gcggcgcgag
     5161 ctgcgccgaa aatccttcgc gccgcaataa aaacaaagcc attcgctcaa tctccgaagg
     5221 cttttccaaa atcgtatttc gccggcggtc ggaatccgtg agaacgtcca gcatctccgc
     5281 ccagctcatt ctgcttttcg acataaaata ggtgagaact gataacagag aactgatagt
     5341 tttttgtttt ttcgctatca gttctctgtt atcaactatc agttattaaa ggctttcaaa
     5401 acttcgtcgg cgtgttcgtc gacgttgacc tttttgaaag tctttttgat tttgccctct
     5461 tcgtcaatca gaaacgtcgt gcggttgatg cccatatatt ttttgccgta catatttttt
     5521 tcgccgtaaa cgccgtaagc gctgacgatt tctttttccg tgtcggcgag caaatcaaaa
     5581 ggcaagctga atttcgagat aaatttccgg tgagaggctt cgtcgtcgtt cgaaacgccc
     5641 agaactttta tgcctttttc cgtgaaaaca tcgtaggaat cgcgcaaaga acacgcctgt
     5701 ttcgtgcagc cgggcgtgtc gtctttcggg taaaaataca aaaccacacg ttgcccgcga
     5761 aaatcagaca gcttgacctg tttgccgctc tggtcggtgg ttgtaaaatc gggtgcggtg
     5821 tcgccttcgt tcagcatatt tctccagttt gttgtaaaat tttaaaggcg tcgaaaatgc
     5881 aaaacgtaaa atgcagaacg caaaatgcaa aacgcaaaat gaaagcggag atttaatcct
     5941 gaattcggaa ttttgaattc ggaattcgga atcctgaatt ttacgctttg cattttcgac
     6001 gcttaagttt ttaaattgta gcaaatgaaa acgctttcgg aaaaacaagc gctgctgaaa
     6061 ctcggaaaac taaaaaacga ttttcaaagt ttcataaaag aaaattatga gcgccgcgca
     6121 aaggattgtc tgacctgccc ggcgcagggc gagtgctgca cggacgcgca ttttgtcaac
     6181 gttcacatca cgcgcctgga agcggcagcc gctcgcgaag ctttgcgaaa atttgacgag
     6241 gcgaaacaaa aagaaattta caaacgcgcc gccgaagcag tcgaaaaata cgatttaaaa
     6301 gattcgggcg acactttcgc gcaaactttc gcctgcccgc ttttcgaaaa aggcgtcggt
     6361 tgtctgattc acaaggaagg aaaacccgcg ccctgcatcc agcacgcctg ttatgaacgc
     6421 cgggcggatt tgccgcccga cgaattacag tctgcggcgg aacaaaaaat cgaacggctc
     6481 gacgcgcgag cttaccggca aaacacaaac tggctgccgc tgccgctctg gctcagccgg
     6541 ataaatccgt ttgattaatt caaacgcgct ttctttaatt ttccgccgag aatcgtcatc
     6601 ggaatcagca aaagcaaaaa caaaatgtga taccagagcg gcgcgtaatt ccagaaaatc
     6661 gactggataa aaatcccgaa caataaaagc aaaacgccgc aggcgagcgt cgatttcgag
     6721 ttttctccgg cgattaaagc cgcgataaaa ccggcgacta tcgaaaaaat cacgcttcgg
     6781 acgagcccga ttatcaaaat cgtcgaatcg gacataaatg ccgttttatt attcaccgcc
     6841 gcttccagtt cggcgtgatg tctgccgtac cagccgggcg aaatcgccga aaacagtgcg
     6901 tcgcttccga cccagagaat cgaccagacg ataaatccga taatcgcgcc caaaattatt
     6961 cgcaacataa attttctcct tcaattttct tcaatgtgtg cccgaaattt aaaactgaca
     7021 gccgatttca gccgataaaa atcgtttcgg aatagatgaa aaccgatagt cgaatagata
     7081 aaagctttca gttatcagcc gtttcgagcc cggtttttca tttcaatgaa attcgctcta
     7141 actatttcga tacctaatct aaatcttgcg gaggatattt gagaggaaat ttacgattcg
     7201 tcctgagaaa ggaattttat aaacggaaaa agggaaagaa cttgctcccg aaatttcttt
     7261 ccctttcctt tttcgccttt tgccagtgcg aaactttaag ctactttgac gcgtttggcg
     7321 tttgtttttt cgctcgccgc gacgtcctga tttttcgttt tttcgatgcg cgccgtcatt
     7381 cgtctgtcca gatcggcgat ttgttccgtc gtcaggtgat aaatgttcgg ctggttatga
     7441 taaatcttcg ctcccgtcac gatgtcggct tttttgaaac cgttcgcttc ggctttggcg
     7501 acgagctcgt caatcgtcag agtattcggc acgagcgaga gcggctgccg ttcttcgata
     7561 aattcgggaa ctttcgacgg cgcgggtttt tcaaacgcca gctctaaagc gtgttcgccg
     7621 cgcatatggt cgagaaaatc cttgaacgtc acgtttccga cgaactcgta accgacttca
     7681 aaggcgggcg aattcgattt gacgacctga aaaacgcgga cgatttcgcc gtcgcgttcc
     7741 tgcaggctga cttcgaccat cacatcgaca aaatactcca gaccgctcgc cgtcatcggc
     7801 agaacgcgcc cgatttcgtt gtcttctttg gcttccttgc ctttggcggg aatctggtcg
     7861 gcaatgacaa tcgattttcc cgattcgatg cagagttttc tcagaacgcc ctgcaaaatc
     7921 atctggtcag ccgccagctc ttcggcgctc ggctgcgcca gcgggtcgcc cgctttttcg
     7981 cgcacggctt caatcatatc cgagtgcttg gcgccgaaat acgccgccca cgaatcgagc
     8041 gcgaaacagc cgtagttctg ctcgcgtcct tcaccgttaa tcgcccagtc gataaattcc
     8101 ggaagctcgt ccgtgttttc gatttccagc gcgtcaaact ggacgccgac gccgcgcaga
     8161 agacgcgctt tgcgctcggt gtcaaaaata caaagttttc ccaagcccgc ttcgctcatc
     8221 gagcgaataa aatggctttt gcccacgccc gcgccgccgc gaatcgccat taccaggcgc
     8281 tgctgtcgcg gtatgcggct caacggattg attttcggtt tcgtcataac tttacttctc
     8341 cttacttttc tcttgtgcca gctaattctt tactttagat tccgtccatt aagtgattac
     8401 catcaaaggc aatccgtttc attgttgaaa caaatctacc ataacttgtt tcatgtttca
     8461 agtatgaaac aaagattttt tgaaaaaatt tacgggttga gaaaaattaa agaagttaaa
     8521 tcgaaataat tacgagtaat ccaagctgtt atttgaagaa tttttgcccg cgaataaacg
     8581 cgaaacagcg cgaaacaaac agaaatgatt tggttttcgt ttccatttgc gcgacttttc
     8641 gcgtttattc gcgggcaaaa caaatttttt ctaattattc gcggctgaat ttatttcaca
     8701 aactcaaact cgccgaacgc ttcgggcacg tgaaacgcgg gctgcggcgt ttcggtcggc
     8761 tgccacgcca gatagccgcg cgtttcgccc gcgccgacgc agcgaaacaa atttccgcgc
     8821 cagacttcat tcgctttcgg agttttgccg aacgctttaa attcgatttt catcgccatc
     8881 aaaactttgt ctttttcaat gcgcgccgcc gcgagcattt ccgaatcgta ttcaaaatcg
     8941 gtttcgcgcc tttccgccaa ttggtgaatc gccaaatcaa tccattcgcc cgtcggcgcg
     9001 atttcaaact cgaaatatct tcgcggctcg cttttatccg gcgcgacgaa aatctcgcaa
     9061 acgtctctgt cccagagccc gcgcgttttt gtttttaaat tcggagtttc gctaataatt
     9121 aaaggctcgg cttgattggc ttcaaagcga acatagagcg ccgtttccga ccacaaaagc
     9181 cgcgctttcg cgtgtctgcc gcgcggcgca tttccgcctg accagtattt ttcgatggcg
     9241 accgtttgtg atttttccca ataattacta tcgagttcgc tgacggaaaa gtcgttttcg
     9301 acgtgtgcga tttttatttt gccggtcatt ttggattgcg aaaaacaaag atgaaaattt
     9361 tacgaccaca aatattttct aacatcaatt aaccttttga gcatttgatt gtcttcttca
     9421 tatcgtcgtt ttattttgtc gaaatcttcc tcgtcgtgaa atcttttcag ccaccaatta
     9481 taaagaaaat ctatttcagc cttagctttt tcggtttctt cgtcgtgttt ccaatcaatt
     9541 tgctcaaata tttccttttc ccgctcgaca aaatctttca gcaattgaaa acaggcgtga
     9601 agaattattt catctttatc acgccaatcg tccggcaggc tttcaatttt aagaatattt
     9661 gccggttcca caacctactt attttctcga attccaaatc gttttggctg ccattttcgc
     9721 cagcttccag cttctgtccc aatcgatttt gccgtcttcc gtatcgcgcc ctaaaatctt
     9781 atcggtcaga agtttgcgta aatccttgcc ttcgcgccgg agcataaatt ccttggcgta
     9841 aggcttcgcc gtttcaaaga aattaaattc cgggtcggtg atgatgccga tgccttcgag
     9901 cgtcatcagc gcgcgcataa tatacgtgaa attcgacggc aggcgaaacg ggtagtcata
     9961 catcacgtcc gccaaatcgt aggtcaattc cttgaaattc acttccttga gcttcagatt
    10021 caaatgaaac tgaaacattt tttcgacgac cgggcgcacg atgtccgggt ttgagccggg
    10081 cttcataaaa tccaaatcaa tcaaatcctg cgcgattccc gccgggtctt tggcgacaac
    10141 gtgaaaaaac gcgtcgatca ttttcgactg tagctgcggc gtaatgcgtc cgaccattcc
    10201 gaaatcgaaa aacgccagtc gcccgtcggg catcaccaaa agattgcccg gatgcgggtc
    10261 ggcgtggaaa aatccgtctt ccagaagctg tttcagataa gtgcgaatca aaagtcgatt
    10321 gactttttcg ggcgagacgt ttctggcttt caattcgtcc aaagcgacga ctttcgtgcc
    10381 gtgaataaac tccatcgtca gaactttttc gctcgtcgcg cgccagtaaa tcttcggcac
    10441 gtgaacgttt gtccaatcct tgaagctttc ctgaaaacgc tcggcgttgc gaccttccgc
    10501 cgcgtaatcc atctcttcgt gaatcgtctc gtcaaattcg cggagcattc cagcccaatc
    10561 ggcgttttcg ttcagagacg gaaatctttc ggcaaatttg gtgacttttc ttaagatttc
    10621 gatgtcgcct ttgatgattc cggcgagatt cgggcgctgg actttgacgg cgacttcttc
    10681 gcccgtaaaa agcttggcgc gataaacctg tccgagcgac gcggcggcaa tcggctcgac
    10741 gtcgaattct tcgtaaactt cgtttatctt tttgcccagt tctttttcga tgcgtgcaaa
    10801 agcgatttca ttcggaaaag gcggcacgga atcctgcaat tctcccaaag atttgacgaa
    10861 cggcaagggc agaaggtcgg cgcgcgttcc cagggattgc ccgattttta taaaagtcgg
    10921 acccagattg attaagtttt ctttcagcca gacggcttgt ttttcctgat tcagctcgcg
    10981 gttcgtttct ccgccgatga aaaagcgtcg aaccggaagc ataaaccggt gaaagaaaaa
    11041 aagtctgatt ttgtaaagat attcgcccag aacggctagc cacgtcaggc gttttgccgt
    11101 ctcaaaccgc gcttcggcgt gtttttcgtg ctgcgcgtga tgcaggtcgt attggtcgag
    11161 gtaaagataa agcgagagca tcccgatgac gcgcgagacg tgaaaaagcc gcagataacc
    11221 gcgccagccc tcataatttt tctcgaccga ttcttcctgc gcaagaagag atttttcttc
    11281 ggcaatcagc tgctcgttgg aaatctgctt tgaagccgga agcgctcgat ttcccgccgg
    11341 agcgtgattg ccgtcggcgg cgggaatcgc ttcaatcgag ttttctttca gcattaaagc
    11401 ctgcttgttt tcttccatcg ccgaatcctt ttgcataact tacttagaat cacaaaaaac
    11461 cgttcggttt gcaaaaattt tcacaaagtt tctatgaaac tttttttcgc gccggattaa
    11521 agccggtagc cgtcgtccgt cataaaacgc aaattccggc attttatatt caaattacta
    11581 acgccgtaag aaatacgtga tttccccgcg agttgttccc ggcaggcgtt ccggttcatc
    11641 cgggctaaat tatatccagc gaattattta cgccctgacg cctgaaataa agcaacgcgc
    11701 gtttggtgtc tttttcgatt tgcgttttaa gctcgtcgat gccgccgaat tttttttcgt
    11761 cgcggatgcg atgcagaaag cggacgcgca ggacattgcc gtataggtcg ccatcgtagt
    11821 cgaagacgta ggtttcaatc gacggttcga tttctttttc aaaggtcggg cgcacgccga
    11881 cattcgtgat gctgcgccgc cattcgccgg cgattaaagt agcggtcgcg tagacgccga
    11941 atttcgggac gacgcgattt cgcggtttca ggttcgcggt cggaaagccg atggttcgcc
    12001 cgcgctcggc gccgtgaatg atttgccctt cgatgccgta aggtcgcccg agcatccggc
    12061 gcgtcagatt gattttcccg acggctaaaa gcgcgcggat ttttgaagaa gaaattcgcg
    12121 cgccgcgcag gcgcacttcc ggcacttcgt cggcgtgaaa accgagcccg gtgcttattt
    12181 gccgcagtaa atctatattt ccggctcgat ttttaccgaa agcgaagccc ttgccgagat
    12241 aaacttcttt tgcctgcaag cgttcgtgaa cgacgttctg caaaaactcg cgcgcgtcct
    12301 gcgcggcaaa ttcacgatta aaacggatga cgatcgcctg ctcgatgccc agaacctcga
    12361 aatttgcgag ccgctggtcg agcgtctgaa gcagcggcgg cgcgctttcg ggatacagaa
    12421 cggcgcgcgg gtgcgggtca aacgtaatgg cggtcggcac ggcgctcgcc gcgcgggcgc
    12481 gctcgacgac cgtctgcata atacgctggt gaccgagatg aagcccgtca aaaacgccga
    12541 gcgtcaagac cgtcgggcgc gagatgtttg cgttttccgt tccgtgaaaa attttcattt
    12601 caaattaaaa aattcggcta tagctcaatc caatattgag aaatcagttt tcccgttttt
    12661 tcgtaaatta tttgtttatc aagccgcccg ccgttgtttt cgataactct cgccgacgcg
    12721 acgttatccg catcgcaggt cagcaaaacc tttttaagcc cgatttcgcg cgctttttcc
    12781 agcacgagcc gcaaaatcag cgtaccgaaa cccctgcctc tttcagacgg gcgaatatca
    12841 tatccgatgt gcccgccgat gatttcgagt tccgcgttca aactgcggcg aagcgcggcg
    12901 cgcccgatta acctgccgtc gccgagcaga taaaattcgc tcgacggcac gcgctcggca
    12961 ggaagattga cgcccgaagc gaacattgcc aaccgctgaa gataagcgtc gaaatcttta
    13021 acgcccgcgt cgtaacgatt ttctccggcg gattgataat cagccgccat ctctgaaaag
    13081 tgcggtttta attttgcgct taactcgacc aaaactgcgt tttgctgatt tatagccacg
    13141 ttattttttc aaataaagct tcattcccag atgcgagcct ttaaagccta aattttcgta
    13201 aaaacgatgc gcgtccgcgc gctcggcgtt cgtcgtcagc tgcacggcaa aacagttttc
    13261 ttcgcgggcg cgttttatag cccaaagcat taattttttg ccgatgccgc gacggcgata
    13321 tttttcgtcg actctgaccg attcgatcgt cgcgcgtttt ccgccctgaa agctgattga
    13381 cggcgtgaaa gtgatttgca gcgtgccgag gatttcgccg cccgtttcgg caacgattag
    13441 ctcgttgttt ttgtcggctt cgatttcctc aaaggctttt atataacttt ccggcaaagg
    13501 attttcgtat cgctcgcgcg cccgtccgag aaaatcgtcg gagagcatcc ggacgatttc
    13561 cggtaaatct tcgcgcgtcg cccggcggaa tttaattttc atatttattc gacgattaat
    13621 ccgtcataag caaactgcac gttgtcgggc agcaggcgcg aatcgcgctc gtgcagaata
    13681 tcgtgattca gatgcgttaa gaaggcgcgt ttcggcttta gtttttcgat gtattcgagc
    13741 gcttcgctca agcccagatg cgtcgagtgc ggttttatcc ggacgcagtc cagaacgagc
    13801 acgtccaaat ccgtcaagcc ccgcatcgcg tcttcgggaa taaattttaa atccgtcgcg
    13861 taggcgaaat cgttaaagcg ataagcgacg accggaagct tgccgtgaat gacttcgagc
    13921 ggcgtaatga gcaaatcttc gccgatgcag aaaggcgaat ttttgacgac cgtgtgcgga
    13981 atcagctgcg gcagaccgcc gccgaaatgc gtcggctgaa agatgtattg aaaaatgcgc
    14041 cggaggtctt tccaggcaat ctcgttggcg tagatgccca tcgcgccgta gcgaaaattg
    14101 agcggtcgga tgtcgtccaa gccgaaaacg tggtcgacgt ggcaatgcgt aatcaaaacc
    14161 gcgtccagat aatttatacg cgcgcgaagc gcctgttggc gaaaatctat cgaggtatcg
    14221 accaggattt tttttccggc gtgctcgatg gtcacggaaa cgcgcaggcg tttgtccttc
    14281 gggttgtccg aaaggcaggt ttcgcactcg cagccgattg acggaacgcc cgtcgaagtg
    14341 cctgtgccga gaaaagttaa tttcataagt tgactgggtt gactgggttg accaagttga
    14401 ccgagtgagt tggttaagtt tactaagttc ataataactc agccaacttt cactcaactt
    14461 tcagtcaact cggtcaactt tttttctcaa gtattgagcg agagaaagag tcatcctcga
    14521 aacttcgtcc aacatttcat aagcttctct gaattcagtt tgctctatat aattcaaatc
    14581 aagcgcgacg taaagatgtg attgaagctc aattgccgaa cctctggcga tattaagaaa
    14641 attggcaaac tcggtagttg tgcgtcgtcc gtgtccttcg gcgatatttg ccataatcga
    14701 aacgctcgcc cgtttcatct ggtcgcgcag actaaaatct ttggaaaaat tatctttatt
    14761 cgacattgaa taaatctttt tcgtaatttc tctagccttt tgccacgcct gaatttcttc
    14821 aaatcttttg aaagtcgaca taataaattc tccgagtcag ttattttatt caagtttgga
    14881 ttaacaaact ttcagtcaac ccggtcaact cagtcaactc gccaaactat tttttaccga
    14941 gaatatcgct cgccgaaaat ttctgcgccg cctgctgttt caatttttct tcggcgaggc
    15001 gcatcatctg cacatactgc atgcgcaaat cgcccagaat tccgtcgaaa agctcctgtt
    15061 ctttcgggtg cagattgttt t
//
LOCUS       DASWNE010000253         2358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_43151, whole genome shotgun sequence.
ACCESSION   DASWNE010000253 DASWNE010000000
VERSION     DASWNE010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43151"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(264..1112)
                     /locus_tag="VGB68_05285"
     CDS             complement(264..1112)
                     /locus_tag="VGB68_05285"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEY0048675.1"
                     /translation="MSEQNKEKMLELLSGKAFSDLSEAELAKLAELEKLFPELQADDS
                     FEIAASAVSLTNLDVSEPLPAHLQAKILADADKYFAAPKASVPVQEATKEEYQKTFAF
                     EPKRSPWQWLGWAFAAFACIALAVNVWTTRFQPREIAGNATPTPAITPQKLSPVQERE
                     QLLNSVDTVQTILTNPKNPNEVLGDVAWSDSAQKGFVRLRGVPVNDAAREQYQLWIFA
                     SNQDPKTPVDGGVFDVNESGEVIIPIDAKIKVQKPTLFAVTAEKTGGVVVSKQEKVMA
                     IAKVET"
     gene            complement(1109..1672)
                     /locus_tag="VGB68_05290"
     CDS             complement(1109..1672)
                     /locus_tag="VGB68_05290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY0048676.1"
                     /translation="MARSILQRIAAKDKTAAQECLNAYGGLIWSLARRMCPNINDAED
                     AVQEIFIDIWKNAERFDQAQASETTFVAMIARRRLIDRLRKSQRQPHVDSIEDMTVEP
                     VKAQDKQMQTAVEASQAAEAMNALRPEQRQVLQLSIYQGMSHQEIADATGMPVGTVKS
                     HARRGLMQIREVLGLGGSGNERKGVIA"
     gene            complement(1893..>2358)
                     /locus_tag="VGB68_05295"
     CDS             complement(1893..>2358)
                     /locus_tag="VGB68_05295"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEY0048677.1"
                     /translation="FCRSELKMSSSEKIFNPFKKFAIAAAIVALCGLIDTIYLTIHHY
                     TARPVPCSLLEGCEMVLTSPYAKMAGVPLALYGAAAYLAAFLLAILAAFVNRKFWRLF
                     GIQVILMAAFTIWLLYLQGMVIGAFCQFCLLSALVTFTLFIIALASRFWRSN"
BASE COUNT          553 a          628 c          573 g          604 t
ORIGIN      
        1 gtcgccgctc tgtgctgaaa tcatcgttgt cataaacgaa acaaggacga acaaagatac
       61 gagaaatttt atagctttca tcgcaaactc cttttcaatt tttcgacgca gaggcgcatt
      121 gggaaacaag acgaaaggca aaagtaaaaa gtaaaatcgt ttcgttttac ctttttactt
      181 tttacttttt actttctgat tctctgtgac tctgagtctt tatgatttga ttttactaat
      241 tttatagacg taagggaact gttttacgtt tcaactttcg caatcgccat tactttttcc
      301 tgtttcgaga cgaccacgcc gccggttttt tcggcggtga cggcaaagag cgtcggtttt
      361 tgaactttta ttttcgcgtc aatcgggata atgacttcgc cgctttcatt gacgtcgaat
      421 acgccgccgt caaccggagt tttcgggtct tgattcgagg cgaaaatcca gagctgatac
      481 tgctctctgg cggcgtcgtt gaccggaacg ccgcgcaggc gcacgaagcc tttctgcgcc
      541 gagtcgctcc aggcaacgtc gccgagaact tcgttcgggt ttttcggatt cgttaaaata
      601 gtctgcaccg tgtcgaccga gttcagaagc tgctcgcgct cctgcacggg agataatttt
      661 tgcggcgtaa ttgccggcgt cggcgttgcg tttccggcaa tctctcgcgg ctgaaagcgc
      721 gtcgtccaga cattaaccgc cagcgcgatg caggcaaacg ccgcgaacgc ccagccgagc
      781 cactgccagg gcgagcgctt cggctcgaaa gcaaaagttt tttgatattc ttctttcgtc
      841 gcttcctgaa cgggaacgct cgctttcggc gcggcgaaat atttatcggc gtcggctagt
      901 attttcgcct gcaaatgcgc gggcagcggc tcggaaacgt ccagattcgt caggctcacg
      961 gctgaagccg cgatttcaaa agagtcgtca gcctgtaatt cgggaaaaag tttttccagc
     1021 tccgccaatt tcgccagttc cgcttcgctt aaatcggaaa aggctttgcc ggaaagaagt
     1081 tcaagcattt tttctttgtt ttgttcgctc atgctattac tcctttcctt tcattaccgc
     1141 tgccgcctaa tcccaaaact tctctaatct gcatcagccc gcgccgggcg tgcgatttga
     1201 ccgtgccgac cggcattccc gtcgcatcgg cgatttcctg gtgcgacatt ccctgataaa
     1261 tcgaaagctg caaaacctgt cgctgctcgg gtcgcagcgc gttcatcgct tccgccgcct
     1321 gactggcttc gaccgccgtc tgcatttgct tatcctgcgc tttgaccggc tcgaccgtca
     1381 tatcctcgat tgaatcgacg tgcggctggc gctgcgactt cctgagccgg tcaatcaaac
     1441 gccgccgcgc aatcatcgcc acaaacgtag tttcagatgc ctgcgcctgg tcgaaacgtt
     1501 cggcgttttt ccaaatatcg ataaaaattt cctgcacggc gtcttccgca tcgttaatat
     1561 tcggacacat ccgtcgcgcc agcgaccaga tgagtccgcc gtaagcgttc aaacattcct
     1621 gcgcggcggt cttatctttc gcggcgatgc gttgaagaat ggagcgagcc aaaatcttta
     1681 cccttttgat agatttaaaa aaacatttct aaactacttc atttcatcat cgcttgccaa
     1741 gttaaggcag ctccgaaaac cgaagtttgc agcgccgcta cttaatatat tcgctgcttt
     1801 tgcggggctg gatgcaaagg atgataaccg gtaaccgata actgaaagaa atcttaattt
     1861 tcagttatcg gttatcggtt accggttatc agttaattcg agcgccagaa gcgcgaagcg
     1921 agagcgataa taaaaagcgt aaacgtcacc agcgccgaaa gcaggcagaa ttgacaaaac
     1981 gctccgatga ccattccctg cagatacaaa agccagatgg tgaacgccgc catcaggatt
     2041 acctgaatac cgaaaagccg ccagaatttc cggttaacga aagccgccag aattgccagc
     2101 agaaaagcgg cgagatacgc ggcagcgccg tacaaagcca gcgggacgcc agccatcttc
     2161 gcatacgggc tggtcagaac catctcgcag ccttcgagaa ggctgcacgg cacggggcgc
     2221 gccgtgtaat gatgaatcgt cagataaatc gtgtcgatga gtccgcacag cgcgacgatt
     2281 gccgccgcga ttgcaaattt tttaaacgga ttgaaaatct tttcggagct gctcattttt
     2341 agttcgcttc gacaaaag
//
LOCUS       DASWNE010000254         3513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_394977, whole genome shotgun sequence.
ACCESSION   DASWNE010000254 DASWNE010000000
VERSION     DASWNE010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394977"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..1411
                     /gene="accC"
                     /locus_tag="VGB68_05300"
     CDS             62..1411
                     /gene="accC"
                     /locus_tag="VGB68_05300"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEY0048678.1"
                     /translation="MKTQKFRKILIANRGEIACRIIWTCKEMGIRTVAVHSEADKDAL
                     HVRFADEAICIGPAPSAQSYLNIPAIISAAEIANVDAIHPGYGFLAESDTFAKICGDC
                     NIKFIGPQSHVIKMMGDKVEARRTMTAAGVPVLPGSPDPIESADEAIRLAKEIGFPVI
                     IKAAAGGGGRGMRIVRAEEDLASNLELAQSEALAAFKNGSVYIERYIERPRHIEIQVL
                     ADEYGNVIHLGERECTIQRRHQKLLEEAPSPVITPEQRAKMGAVAVKACQEIGYSSAG
                     TFEFLLDEDGSFYFMEMNTRIQVEHPVTEMVTLADIVRNQIRIAEGEELGVTQEEVLI
                     VGHSIECRINAEDPEKFTPSPGRITAFNLPGGPGVRVDTAAYPGYVVPPYYDSMIAKL
                     IVHARTRELAIARMKRALEAMVIEGIKTTIPLHLKIMNDEKFQRGDFSTKFMEEFGK"
     gene            1539..1808
                     /gene="rpsO"
                     /locus_tag="VGB68_05305"
     CDS             1539..1808
                     /gene="rpsO"
                     /locus_tag="VGB68_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEY0048679.1"
                     /translation="MATATEVKEQIVKDFRQHGSDTGSPQVQVALLTQRITELTEHFR
                     THKKDNHSRRGLLKMVSQRRKLLDYLKRTNIEQYHEIVQRLGLRR"
     gene            2237..>3513
                     /locus_tag="VGB68_05310"
     CDS             2237..>3513
                     /locus_tag="VGB68_05310"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:NF008805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEY0048680.1"
                     /translation="MPQRKYLKESIKLGEQELTIETGKIAKQADGAVVVRYGDTMILV
                     TAVSAHTAREGLDFFPLTVEYRESTYAAGRIPGNYFRREGRPSEKETLTCRLIDRPVR
                     PLFPDNYRFETQIIGTVISADDQNDPDVIAITGASLALYLSDIPFVNPIAGVRVGLIE
                     GKYVINPTYDARRESQLNLIVAGTEEAICMVESEAKEVSEKIMVEALMLAHKEIKRLC
                     LWQKELFKALEITKREFVAPTVDEALIAEIEKNYGERLRASMDTTGQDKITSYAAIDA
                     LKKETVESYPEDQPEQRSMAGKAFSFLKEKIFREDILGKRRRPDGRRFSEIRPISCDV
                     GWLPRVHGSALFTRGETQAIVTATLGTKDDEQFMDDLEKGETKRRFMLHYNFPPYSVG
                     ETGRVGSSSRREIGHGNLARRAIEGILPSEADFP"
BASE COUNT          928 a          877 c          937 g          771 t
ORIGIN      
        1 ctatttttaa tttttcctga ttttcgtgct atttcgtgtt atttcgtggc taaattctta
       61 aatgaaaacg caaaagtttc gcaaaatttt aatcgccaat cgcggcgaaa tcgcctgccg
      121 gattatctgg acttgcaagg agatgggcat tcgcaccgtc gccgtgcatt cggaagccga
      181 caaggacgcg ctgcacgtgc gttttgccga cgaagccatc tgcatcggac ctgcgccgtc
      241 ggcgcagagt tatctgaata ttccggcaat catcagcgcg gcggaaatcg ccaatgtcga
      301 tgcgattcat ccgggctacg gctttttagc cgaatccgac acttttgcca aaatctgcgg
      361 cgactgcaat atcaaattta tcggtccgca gtcgcacgtc attaaaatga tgggcgacaa
      421 ggtcgaagcg aggcggacga tgaccgccgc cggagtcccg gttctgcccg gctcgcccga
      481 cccgattgaa tcggcggacg aagcgataag gctggcgaag gaaatcggct ttccggtcat
      541 catcaaagcg gcggcgggcg gcggcgggcg cggaatgcga attgtccgcg cggaagaaga
      601 tttggcttcc aatctcgagc tcgctcaatc cgaagcgctc gcggcattca aaaacggaag
      661 cgtttatatc gaacgataca tcgagcgccc gcgtcacata gagattcagg ttttagccga
      721 cgaatacggc aacgtgattc atctcggcga gcgcgagtgc acgattcagc gccgccatca
      781 gaagcttctg gaagaagcgc cgtcgccggt catcacgccg gaacagcgcg cgaagatggg
      841 cgcggtcgcc gtaaaggcgt gtcaggaaat aggttattcg agcgccggaa ccttcgagtt
      901 tttgctggac gaggacggtt cattttattt tatggaaatg aacacgcgca ttcaggtcga
      961 gcatccggta accgagatgg tcacgctcgc cgacatcgtc cgcaatcaaa tccgcatcgc
     1021 cgagggcgaa gagctcggcg tcacgcagga agaagttttg attgtcggtc actccatcga
     1081 atgccgcatc aatgccgaag acccggaaaa atttacgcct tcgcccggtc gcattacggc
     1141 gtttaatctg ccgggcggtc cgggcgtgcg cgtcgatacg gcggcttatc cgggttatgt
     1201 cgtgccgcct tattacgatt cgatgattgc caaattgatt gttcacgcgc gaaccagaga
     1261 gctggcgatt gcgcggatga agcgcgcgct cgaagcgatg gttatcgaag gaatcaaaac
     1321 gacgattccg cttcatttga aaattatgaa cgacgaaaag tttcaaagag gagatttttc
     1381 gacgaaattt atggaagaat tcgggaaatg acggcgaatt gagaatgttt gaccggaaag
     1441 attgaccatt cttgatttaa catttaacat ttaccagtta ataaaaataa attcacgcgg
     1501 tttcgactgc gactaaggag aggctatttt aagaagttat ggctacagct acagaagtaa
     1561 aagaacagat agttaaagat tttcggcagc acggctcgga tacggggtcg ccgcaggtgc
     1621 aggttgcgct tttgacgcag cgaatcacgg aactgaccga gcatttcaga acccacaaaa
     1681 aagacaatca ttcgcgccgc ggactgctta aaatggtctc gcaacgccga aaactgctcg
     1741 attatctgaa acgaacgaat attgaacagt atcacgaaat cgtgcagaga ttgggactgc
     1801 gtcgatagtt aataactgat aactgaaaac tgatagttta tttttttcac tatcaactat
     1861 cagttatcaa ctatcagttg aaaatgaccg cttcgagtca ttcactcata aagttctctc
     1921 gaagaatttg ctcctagttg ctgtacgtga cagtgtaaag ctacgagcaa aagagaatgt
     1981 cgtcaaaatt agattaaacc aaagacggca gaagctttca cggcatggct tctgccgtct
     2041 ttgtatttga aaattaaagc gcgggaacgg gaaagtgaaa agacctggaa ttacaccgcc
     2101 ggggaacacc gagaattcat tgataaaaaa gaagtttgat tcgtgcaatt cgtggcgaaa
     2161 cctttccaga cacccggcgg acatttcccg ccgctcgcgc gttaaaaaaa ctttacgaat
     2221 ggagaaaaaa aagtaaatgc cacaaagaaa atatttgaaa gaatcaatca aattgggcga
     2281 gcaggaattg accatcgaaa ccggcaaaat cgccaaacaa gccgacggcg cggtcgttgt
     2341 tcgttacggc gacacgatga ttttagtcac cgccgtcagc gcgcacaccg cgcgggaagg
     2401 actggatttt tttccgctga cggtcgagta tcgtgaatca acttacgccg ccggacgcat
     2461 tccgggaaac tattttcgcc gcgaagggcg tccgagcgaa aaggaaactt taacctgccg
     2521 cctgattgat cgtccggtca gaccgctatt tccggacaat tatcgctttg aaacgcaaat
     2581 tatcggcacc gttatctccg ccgacgacca gaatgacccg gacgtcatcg ccattacggg
     2641 cgcgtcgctg gcgctgtatc tttcggatat tccgtttgtg aatccgattg cgggcgtgcg
     2701 cgtcggcttg attgaaggca aatacgtcat taacccgacc tatgacgcgc ggcgcgaatc
     2761 gcagttaaac ctgattgttg ccggaacgga agaagccatc tgtatggtcg aatccgaagc
     2821 caaagaagtt tccgaaaaaa ttatggtcga ggctctgatg ctcgcgcata aggaaatcaa
     2881 gcggctctgt ctctggcaga aagagctttt caaggcgctg gaaatcacca agcgcgaatt
     2941 cgtcgcgccg accgtcgatg aagcgttgat tgccgaaatc gaaaaaaatt acggcgagcg
     3001 cctccgcgcg tcgatggaca cgaccggaca ggacaaaatt accagctacg cggcgattga
     3061 cgcgctgaaa aaggaaaccg tcgaaagtta tcccgaagac cagccggaac agcgctcgat
     3121 ggctggaaaa gccttctctt tcttgaaaga gaaaatcttc cgcgaagaca ttctcggcaa
     3181 gcgccgtcgc ccggacggtc gacgcttttc ggaaatccgc ccgatttcct gcgatgtcgg
     3241 ctggctgccg cgtgttcacg gctcggcgct tttcacgcgc ggcgaaacgc aggcgattgt
     3301 caccgcgacg ctcggcacga aagacgacga gcagtttatg gacgatttgg aaaaaggcga
     3361 aaccaagcgc cgctttatgc tgcactacaa tttcccgcct tattcggtcg gcgaaaccgg
     3421 cagagtcggc agttcttcgc gccgcgaaat cggtcacgga aatctggcgc gccgcgcgat
     3481 cgaaggcatt ctgccgagcg aagccgattt ccc
//
LOCUS       DASWNE010000255         3618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_113502, whole genome shotgun sequence.
ACCESSION   DASWNE010000255 DASWNE010000000
VERSION     DASWNE010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113502"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="VGB68_05315"
     CDS             <1..977
                     /locus_tag="VGB68_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY0048681.1"
                     /translation="AFITTTSTSENFFGQSEEGKGSGSGSVIDAQGHILTNNHVIEGA
                     ANLKVSFGGDKVYPAKVVGRDPDTDLAVIKIEPPAEGLTVVPLGDSDKLSVGQKVLAI
                     GNPFGLDRTLTTGVISGLQRPIRARNGRPIDAAIQTDASINPGNSGGPLLDKYGRMIG
                     INSQILSPAGGSVGVGFAIPVSTARRIIPQLIQYGEVRRPKLGANLYSVSQLNEQGVN
                     LPVEGGLLVRSAVMGGAAASAGIRGLSQGADGDVVLGDIILAIDGQKMNNLDDLYRYL
                     DKKQIGDTIQAEIFRDGKRSTVPVKLVALPAQTQPTGSGRPAIRPRNE"
     gene            1059..1304
                     /locus_tag="VGB68_05320"
     CDS             1059..1304
                     /locus_tag="VGB68_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048682.1"
                     /translation="MTRIELDDFYNDGFGWICKHCERELNGSETGSHARSRLMREGEA
                     ESKNPRLSNAALAKWADKAQRTLMCPRCGITELADKY"
     gene            1415..1972
                     /locus_tag="VGB68_05325"
     CDS             1415..1972
                     /locus_tag="VGB68_05325"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0048683.1"
                     /translation="MTKILDVGCGSNKYEGAIGLDNNPRTAADVIHDLGQIPYPFADD
                     EFDLIVSRHVIEHVPDVMAFITELYRISKPGGRIRLVTPHYTNPDWATDPTHRNHFNS
                     YTLNTFMPERQVFDFYTEVTLKPVRIYVSLLNLWRSLGIEFLVNLDQKSPRFRFTRKF
                     WEHYLSYIFRGKELHFEFEVVKKQK"
     gene            complement(2036..2884)
                     /gene="ispH"
                     /locus_tag="VGB68_05330"
     CDS             complement(2036..2884)
                     /gene="ispH"
                     /locus_tag="VGB68_05330"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF002187.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY0048684.1"
                     /translation="MQILLADEYGFCFGVERAVEMVEEALSEGDTVRTLGPLIHNDQE
                     MERLAKHGVATINAPVQIQRGETAVIRAHGVTPQVEQELRGKASKVVDATCPFVTKVQ
                     KLASRAAAQDRHVVVVGNPDHPEMIGVKGYAPEHAFVIRDASEVADLPMLKNPLVVSQ
                     TTIKLKSFLDAAEAVRAKAEGETQVVNTICSATRDRQDAARALAGEVDAFYIIGGRHS
                     SNSRKLLAVCQEQCPKSFLIETPDEINAADLRGAEKVGLTAGASTPNWLIQKVAARLE
                     EIGKEV"
     gene            2957..>3618
                     /locus_tag="VGB68_05335"
     CDS             2957..>3618
                     /locus_tag="VGB68_05335"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEY0048685.1"
                     /translation="MQINYRFLSPHDFAALHAALLEAFSDYIVPFQLTESQFKNHIAL
                     NAVDINNSVGAFSGGKMIGFTLNGFGFWNGKSTVYDAGTGVLPGFRGRGIGAEIFEFM
                     TPVLKQNGIAQILLEVITENEKAVRLYRKMGFEETRRLLLFQQQKPSAYGSNTDFTIR
                     EIAVPDWKLLKAFWDGNTSWQNSAEALERSLAGKIILGAFSKEKCVGYGIVYPKSGNI
                     AQ"
BASE COUNT          844 a          974 c          956 g          844 t
ORIGIN      
        1 tagcctttat taccacgacc tcgaccagcg aaaatttctt cgggcagagc gaggaaggaa
       61 aaggctcggg ctcgggctcg gtgattgacg ctcaaggaca cattttaacc aacaatcacg
      121 ttatcgaagg cgcggcgaac ctcaaggtca gcttcggcgg cgataaagtt tatccggcaa
      181 aagtcgtcgg acgcgacccg gacacggatt tagccgtcat taaaatcgag ccgccggcag
      241 aaggcttaac ggtcgttccg ctcggcgatt ccgacaagct ttcggtcggg cagaaggtgc
      301 tggctatcgg caatcctttc ggtctggaca gaactctgac gaccggcgtc atttccggtc
      361 tgcagcgccc gattcgagcg cgcaacggtc gcccgattga tgcggcgatt caaaccgacg
      421 cgtcgattaa tccgggcaat tccggcggac ctctgctgga caaatacggc agaatgatcg
      481 gcatcaattc gcagatactc tcgcccgcgg gcggctcggt cggcgtcggg tttgcgattc
      541 cggtcagcac ggcgcgacgc attattccgc agctgattca gtacggcgaa gttcgtcgtc
      601 cgaagctcgg agcaaacctg tacagcgtct cgcagcttaa cgagcagggc gtcaatcttc
      661 cggtcgaagg cggtctgctg gtgcgcagcg cggtgatggg cggcgcggca gcgagcgccg
      721 gaattcgcgg gctttcgcag ggcgcggatg gcgacgtcgt tctgggcgat attattctgg
      781 cgattgacgg tcagaagatg aacaatctgg acgaccttta tcgctatctc gataaaaaac
      841 aaatcggcga tacgattcag gcggaaattt tccgtgacgg caagcgctcg accgttccgg
      901 tcaagctggt tgctttaccg gcgcaaacgc agccgaccgg cagcggtcgc ccggcgattc
      961 gtcccagaaa cgaataaaag ccgagataat aataaaaaat caaaagcggg tgaaattatt
     1021 cgcccgcttt tgtttatgct aatctgtcac tctaaagtat gactagaatc gagctggatg
     1081 atttttataa cgacggtttc gggtggattt gcaaacactg cgagcgtgaa ttaaacggct
     1141 ccgaaaccgg ctcgcacgct cgatcgcgcc tgatgcgcga aggcgaagcg gaaagcaaaa
     1201 atccgcgtct ttccaacgcg gcgctggcga aatgggcgga caaagcgcag cgaaccctaa
     1261 tgtgtccgcg ctgcggcata acggaactgg cggataaata ttaaaaaaaa cgatgaatgc
     1321 ggaacgatga gcgatgaatt tgttatgtag aaactgcaat tcatcgttca tcgttccgca
     1381 ttcatcgttt cttcgtgtgg taaattattt ttcgatgaca aaaattctcg atgtcgggtg
     1441 cggaagcaat aaatacgaag gcgcgattgg cttggacaac aacccgcgca cggcggctga
     1501 cgttattcac gacctcgggc agattcctta tcctttcgcg gacgacgaat tcgatttaat
     1561 cgtctcgcgc cacgtcatcg aacacgttcc tgacgtaatg gcttttatca ccgagcttta
     1621 ccgcatcagt aagcccggcg ggcggattcg cctcgtcacg ccgcattaca caaatcccga
     1681 ttgggcgacc gacccgacgc atcgcaatca ttttaattcc tacacgctca atacctttat
     1741 gcccgaaagg caggttttcg atttctacac ggaagtgact ttaaaacccg tcaggattta
     1801 cgtttcgctt ttgaatctgt ggcgcagcct gggaatcgaa tttctggtca atctcgacca
     1861 gaaatcgccg cgctttcgct tcacccgaaa gttctgggag cattatctga gttatatctt
     1921 tcgaggaaag gaattgcact tcgaatttga agtggtgaaa aagcaaaaat aaaaaggcac
     1981 aagcgaagaa agttactttc ttacttttgc cttttacctt tttatttttg cttttttaaa
     2041 cttctttccc gatttcttcc agccgcgcgg cgaccttttg aatcagccag ttcggcgtcg
     2101 aagcgcccgc cgtcaagccg actttttccg cgcctcgcag gtctgcggcg ttaatctcgt
     2161 cgggcgtctc tatcagaaag cttttcgggc attgttcctg gcagacggca agcagcttgc
     2221 ggctattcga cgaatgacgc ccgccgataa tgtagaaagc atcgacttcg cccgccagcg
     2281 cgcgcgccgc gtcctgacgg tcgcgcgtcg ccgaacaaat cgtgttgacg acctgtgttt
     2341 cgccttcggc tttggctctg acggcttccg ccgcgtcgag aaagcttttc agttttatag
     2401 tagtttgcga aacgaccaga ggatttttca gcatcggcaa atccgctact tccgaagcgt
     2461 cgcggatgac gaaagcgtgt tccggcgcgt agcctttgac gccgatcatt tcgggatggt
     2521 cgggatttcc gacgacgacg acgtggcggt cctgcgccgc cgcgcgcgag gcgagcttct
     2581 gcactttcgt cacaaaaggg caggtggcat cgacgacctt ggaagccttt ccgcgcagct
     2641 cctgctcgac ctgcggcgtg acgccgtgag cgcgtataac cgccgtttcg ccgcgctgga
     2701 tttggacggg cgcgttgatg gtcgcgacgc cgtgttttgc cagtcgttcc atttcctggt
     2761 cgttgtgaat cagcggaccg agcgtgcgaa ccgtatcgcc ttccgacagc gcttcttcaa
     2821 ccatctcgac ggcgcgctcg acgccgaaac aaaaaccgta ttcatctgcc agtaaaatct
     2881 gcattttctt aaaccttcga gcttgacttt cgtaaagcta gttgtatctt aacaaaagcc
     2941 cgaaggaaac aaaactttgc agataaatta tcgttttcta tcgccgcacg atttcgccgc
     3001 gcttcacgcc gcgctgctcg aagccttttc cgattacatc gttccattcc aactgaccga
     3061 atcccaattc aaaaatcaca tcgcgctcaa cgccgtcgat ataaacaatt cggtcggcgc
     3121 tttttcgggc ggaaaaatga ttggttttac gctcaacggc tttggcttct ggaacggcaa
     3181 atcgaccgtc tacgacgccg gaacgggcgt ccttcccggc tttcgcggca gaggcatcgg
     3241 cgcggagatt ttcgagttta tgacgcccgt cttaaaacaa aacggcatcg cgcagattct
     3301 gctggaagtc atcaccgaaa acgaaaaagc cgttcggctc taccgcaaaa tgggttttga
     3361 agaaacgcgc cggcttttgc tttttcagca gcaaaaaccg tcggcttacg gttcgaacac
     3421 ggattttaca atccgcgaaa tcgccgtgcc ggactggaaa ttattaaaag ctttctggga
     3481 cggaaacacc agctggcaga attccgccga agcgctggaa agaagcctgg caggaaaaat
     3541 aattctcggc gcattttcaa aagaaaaatg cgtcggctat ggcatcgtct atccaaaatc
     3601 cggcaatatc gcgcagat
//
LOCUS       DASWNE010000256         2005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_148819, whole genome shotgun sequence.
ACCESSION   DASWNE010000256 DASWNE010000000
VERSION     DASWNE010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148819"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="VGB68_05340"
     CDS             <1..669
                     /locus_tag="VGB68_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HEY0048686.1"
                     /translation="LGGHFDNWTDKTGLAEYAAQFKLIIVTPEGADGWYTDSASLPND
                     RYETYITDELIPEIDKKFRTAADRNNRAIAGLSMGGYGAFKFGLKYPEKFVLAGSFSG
                     ALGAVSLNEKNAGAWIAKSINGVFGDEASEARITNDIFRIIREMPAEKAKRLPFFYFD
                     CGTEDFLAANNREFMNLLVEKKIRHEYRQLPGGHNWVYWDAQVQEFLRLSERFFKLAK
                     AKAN"
     gene            715..1170
                     /locus_tag="VGB68_05345"
     CDS             715..1170
                     /locus_tag="VGB68_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048687.1"
                     /translation="MFKKIIWTSLLILSVLVVSAFAQEKTVVNDARARALLLGRHRLS
                     LQWISWDYFGAANVAERNGVLYLKGNQRRRGGSDYLTIDGRINSVDAKEFSFSGKIIM
                     QISHINGGKPCVREGDFTFKITGKRRYWRLQEMDSPCDTTTDYVDIFFR"
     gene            1310..>2005
                     /locus_tag="VGB68_05350"
     CDS             1310..>2005
                     /locus_tag="VGB68_05350"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY0048688.1"
                     /translation="MLNFAIQTARDAGQILLEKFGRNIKVSKKGDINLVTEADLASER
                     LIVERVRSYYPRHSILAEESGESLAVIEGENIWKWIIDPLDGTTNYAHGYPCFCVTIA
                     LEHRGEIVLGVTYDPTRDELFAAEKGQGASLNGRKIRVSDTEQLSEALIVTGFPYDVA
                     QRNDFARHLTDFLLQSRGVRRDGSAAIDMAYVACGRFDGFWEEGLNPWDVAAGLLLIH
                     EAGGRVSYYDDTTF"
BASE COUNT          522 a          503 c          532 g          448 t
ORIGIN      
        1 ctgggcgggc attttgacaa ttggacggac aaaaccgggc tcgccgaata cgccgcgcaa
       61 ttcaagctca tcatcgtcac gcccgaaggc gcggacggct ggtataccga cagcgcgagc
      121 ctgccaaacg accgctacga aacatacatc acggacgagc tgattcccga aatcgacaaa
      181 aaatttcgca ccgccgctga tagaaacaat cgcgccattg ccggactttc gatgggcggt
      241 tacggcgcgt ttaaattcgg tttgaaatat cccgaaaaat ttgttctggc gggctcgttc
      301 agcggcgcgc tcggcgcagt ctcgctcaac gaaaaaaacg cgggcgcgtg gattgccaaa
      361 tcaatcaacg gcgttttcgg cgacgaagcg agcgaagcgc gcataacgaa cgatattttt
      421 cgaatcatac gcgaaatgcc cgccgaaaaa gcgaaacgac tgccgttttt ttatttcgat
      481 tgcggcacgg aagattttct cgccgccaat aatcgtgaat ttatgaattt gctggtcgag
      541 aaaaaaatcc ggcacgaata ccggcagctt cccggcgggc ataactgggt ttactgggac
      601 gcgcaggttc aggaatttct gcgcttgagc gagcgatttt ttaaattggc aaaagcaaaa
      661 gcgaattaac cgattcgtct gaaaacagag gaaatttcta tgtttaaaaa ttttatgttt
      721 aaaaagataa tttggacgag ccttttaatt ctttccgttt tggtcgtttc ggcattcgcg
      781 caggaaaaaa ccgtcgttaa cgacgcgcga gcgagagcgc tgctgctcgg caggcacagg
      841 ctttctttgc agtggatcag ctgggattat ttcggcgcgg cgaacgtcgc cgagagaaac
      901 ggcgttttgt atctgaaagg aaatcagcgc cggcgcggcg gcagcgatta tctgaccatc
      961 gacggcagaa tcaattccgt cgacgcgaag gaattttcgt tcagcggaaa aatcattatg
     1021 caaatcagcc acatcaacgg cggcaagccc tgcgtccgcg aaggcgattt tacatttaaa
     1081 atcaccggca aaagaagata ctggcgattg caggaaatgg acagcccctg cgatacgacg
     1141 accgattacg tcgatatttt ctttcgttaa ggacatatct atatagaaaa ctgccttaag
     1201 aaacacgata gttaaagaat acttttccgg acggtcggtt ttctatttca aaaccgcaat
     1261 cgaaaactcg tccttgacag cctggcggtc ggcgcgttga aattattcaa tgctcaattt
     1321 cgcaattcag actgcccgcg acgcgggaca aattctgctc gaaaaattcg gacgaaatat
     1381 taaagtctcg aaaaagggcg acataaatct cgtcacggaa gccgacctcg cttccgaacg
     1441 tttaatcgtc gaaagagttc gcagctatta cccgcgccat tcgattctgg cggaagaatc
     1501 gggcgaatcg ctcgccgtta tcgaaggcga aaacatctgg aaatggatta tcgacccgct
     1561 cgacggcacg acgaattacg cgcacggtta cccgtgtttc tgcgtgacga ttgcgctcga
     1621 acaccggggc gaaatcgtcc tcggcgtgac ttacgacccg acgcgcgacg agcttttcgc
     1681 cgccgaaaaa gggcagggcg caagcctgaa cggcagaaaa atccgggttt ccgataccga
     1741 acaactgagc gaagcgttaa tcgtcaccgg ttttccatac gatgtggcgc agcgtaatga
     1801 tttcgcacgg catttaacgg attttctatt gcaatcgaga ggcgtccgtc gcgacggctc
     1861 ggcggcgatc gatatggcgt atgtcgcctg cggtcgcttc gacggtttct gggaagaagg
     1921 cttaaacccg tgggacgtgg cggcgggact tcttctgatt cacgaagcgg gcgggcgcgt
     1981 ctcctattac gacgacacga ctttc
//
LOCUS       DASWNE010000257         1743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_148887, whole genome shotgun sequence.
ACCESSION   DASWNE010000257 DASWNE010000000
VERSION     DASWNE010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148887"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VGB68_05355"
     CDS             complement(<1..291)
                     /locus_tag="VGB68_05355"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0048689.1"
                     /translation="MKINEIPVSAEQLRVMLETGYILREASRFDEAEAVFRGVMEFLP
                     DSEVPQVGLGTVFLQRGDFASAQKICQQALQTQPSSLYARVHYAEALLFSRQR"
     gene            complement(409..873)
                     /locus_tag="VGB68_05360"
     CDS             complement(409..873)
                     /locus_tag="VGB68_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048690.1"
                     /translation="MAYNDPLVTPTGQQLPPMSTVSPETSIMRPQPKEPNKFLKVLGG
                     IAGGALNLVAPGLGGILGNVIGGGGSSVSSYSALMEQQRQFEEMQRYNQISTALQQKQ
                     QNSMMNQQQQQSMQLLGVQHRVSMQSQEFTTVSNLMKTRHDSEMSAVNNIKS"
     gene            complement(1221..1511)
                     /locus_tag="VGB68_05365"
     CDS             complement(1221..1511)
                     /locus_tag="VGB68_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048691.1"
                     /translation="MQNKQSADNSAADSIGSQFTEEDLAKLYDYPSIGQLFSEPDTRR
                     LDEFCLRLRTTRENLERVVRYGDRSEAERAARAIRAVEVTLDFLQSLQKMRQ"
BASE COUNT          416 a          429 c          427 g          471 t
ORIGIN      
        1 gcgctgccga ctgaaaagga gtgcttcggc gtaatgaacg cgggcataaa gactggaagg
       61 ctgggtttgc agggcttgct ggcagatttt ctgcgccgaa gcgaaatcgc cgcgttgtag
      121 gaagaccgtt cccaatccga cctgtggaac ttcggaatcg ggcagaaatt ccatgacgcc
      181 gcgaaaaacc gcttctgcct cgtcaaatct cgaagcctcg cgcaggatat aaccggtctc
      241 gagcatcacg cgcaactgtt cggcgctgac tggaatttcg ttgattttca taagaactta
      301 ctcgaaggac gaaaatcggc agacgcgcac gctttgaaat gacatcggat tttacttaaa
      361 aaacctgaat ccggcgcgtc tgccgaaaat tataaaccgc cgtaaatctt acgacttaat
      421 gttgttgacc gccgacatct cgctgtcgtg gcgcgttttc atcaaattgg aaacggtcgt
      481 aaactcctgc gactgcatgc tgacgcgatg ctgaacgccg agaagctgca tcgattgctg
      541 ctgctgctgg ttcatcatcg agttttgctg tttctgctgc agagcggtcg aaatttgatt
      601 gtaacgctgc atttcttcaa actggcgctg ctgttccatc aacgcgctgt aggagctgac
      661 cgacgagccg ccgcctccga tgacgttgcc gagaatgccg ccgagtccgg gcgcgacaag
      721 atttaaagcg ccgccggcga tgccgcccag aactttcaga aacttgttcg gttctttcgg
      781 ctgcgggcgc ataatgctgg tttcaggaga aaccgtgctc atcggcggaa gctgttgtcc
      841 ggtcggcgta actaatgggt cgttgtatgc cataatcaat tcacctctcc caaattttca
      901 ggctttcccg ctttaaaata cggcgcggaa agctaaatat ttttattcct tgtcattgtt
      961 atcggcagat tctctgaaat gtttcctgtt tttttgtcaa aatttgtaaa aatatttcta
     1021 acgccgggaa ataaatttat gggcgctccg gtgtctgcgc aaagcgcgtg tcgagaggct
     1081 aatcagtttt aataaaattc aatgttgatt tttattctaa acacttattc gccgtgctcg
     1141 ccggaccggc gctgcggcgt gcccatacaa tttccggcga gattttcaat gatttcccga
     1201 actgcgtttt ttaatttgac ctattgacgc attttttgca gcgattgcag aaaatccagc
     1261 gtcacttcga cggcgcgaat ggcgcgcgcg gcgcgttcgg cttcggagcg gtcgccgtag
     1321 cggacgacgc gctccagatt ttcgcgcgtc gtccgcagac gcaggcaaaa ttcgtccaga
     1381 cgtcgcgtgt ccggctcgct gaataattgt ccgattgaag gataatcgta aagcttcgcc
     1441 aaatcttcct cagtaaactg cgacccgata gaatccgccg ccgaattatc tgccgattgt
     1501 ttattttgca tctcgaatac ttaccccgtt ttataaaaac tcgaaagctt cttttccggc
     1561 tcgaccggat tattcataaa aataatttcg gcgcgctgct cgcattaact ggaaaataag
     1621 cttgtgttac tatttctata ttataggatt ttgtcgcaaa attttataga tttttcatga
     1681 cgaaaagatt actggtcaaa aaacacggga aaattgtttc cgagctcacg cgggaaatca
     1741 gcg
//
LOCUS       DASWNE010000258         1969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_255902, whole genome shotgun sequence.
ACCESSION   DASWNE010000258 DASWNE010000000
VERSION     DASWNE010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255902"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="VGB68_05370"
     CDS             complement(<1..727)
                     /locus_tag="VGB68_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048692.1"
                     /translation="MKLRLFFAIFSIFLLGAAISAQTKGKYANLFSEEKLKSTVKFLS
                     DDAFEGRAPGSRGGELAAKYIANQLQQLGIKPGNNGSYFQPVSLVAVRANPSTMLNVG
                     NDSYKFGSEFVAFTGAQTENVNLNAELVFVGYGIDAPEQKWNDYKGNAEDFRGKILVM
                     MVNDPPATPEEPNLFGGKGLTYYGRWTYKFEEAARRGAAGVLLIHTTESAGYGWNVVQ
                     TSNGSWRFDVARTPEDKTPFLQMR"
     gene            complement(762..1337)
                     /gene="folE"
                     /locus_tag="VGB68_05375"
     CDS             complement(762..1337)
                     /gene="folE"
                     /locus_tag="VGB68_05375"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEY0048693.1"
                     /translation="MAKKKKVDLEKIARGVRLILEGIGEDVDRDGLQKTPERVADFYA
                     ELTEGMWEDAREHIVPLPGDSHDEMVIVKDISIASVCEHHLAPFVGKCHIAYIPKGGR
                     IVGLSKLARLAEIFARRLQVQERLTQQIAQTLFEELNPLGVMVVMEAEHTCMTLRGVK
                     KPGAKTITSSVLGGFRTDARTRSEAMSLIKG"
     gene            complement(1551..>1969)
                     /locus_tag="VGB68_05380"
     CDS             complement(1551..>1969)
                     /locus_tag="VGB68_05380"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048694.1"
                     /translation="DSFVDSYDDLRKALPAVKLVQVVHVGGEESLREAIEISEKVDAI
                     LLDSGNQALEIKELGGTGRTHDWTISRKIVEGVKSPVFLAGGLKAENVAEAVEKVQPF
                     GLDLCSSVRTEGKLDEEKLKRFFAEVDAFNAKSPSH"
BASE COUNT          444 a          560 c          461 g          504 t
ORIGIN      
        1 aacgcatttg cagaaaaggc gttttatctt ccggcgttcg cgccacgtca aaacgccaag
       61 agccgttgga agtctgcacg acgttccagc cgtagcccgc cgattcggtc gtgtgaatca
      121 aaagcacgcc cgccgcgccg cgccgcgccg cttcttcgaa tttatacgtc cagcgcccgt
      181 aataagtcag ccctttgccg ccgaaaagat tcggttcttc gggagtcgcg ggcgggtcgt
      241 tgaccatcat cacgagaatt tttcctctaa aatcttcggc gtttcccttg taatcgttcc
      301 acttctgttc gggcgcgtcg atgccgtagc cgacaaaaac cagctcggcg ttaagattga
      361 cgttctccgt ttgcgcgccg gtaaaggcga cgaattcgct gccgaatttg tatgaatcat
      421 tgccgacatt cagcatcgtg gaaggattgg ctctaacggc gaccagcgaa accggctgga
      481 aatacgagcc gttatttccc ggcttgatgc cgagctgctg caattgattc gcgatgtatt
      541 tcgccgccag ctcgccgccg cgcgagccgg gcgcgcgacc ttcaaaagcg tcgtcggaca
      601 gaaatttaac ggtcgatttc agtttttctt cgctgaaaag attggcgtat ttgcctttgg
      661 tctgtgccga aatcgcggcg ccaagcagga agattgaaaa tatcgcaaag aataaacgca
      721 gtttcataag ttttgccctt taacttttat ttttgcctta actaaccttt tattaacgac
      781 atagcttcgg agcgcgttcg cgcgtcagtg cgaaatccgc cgagaacgct cgaggtaatc
      841 gtcttcgcgc ccggtttttt cacgccgcgc aaggtcatac aagtgtgttc ggcttccatt
      901 acgaccatta cgccgagcgg attcagctcc tcaaaaagcg tctgcgcgat ttgctgcgtc
      961 aggcgttcct gcacctgcag tcgccgcgca aaaatttccg ccagccgcgc cagcttcgag
     1021 agtccgacga tgcgcccgcc tttcggaata tacgcgatgt gacatttgcc gacaaacggc
     1081 gcgagatgat gctcgcagac cgaggcaatc gaaatgtctt tgacaatcac catctcgtcg
     1141 tgcgaatcgc ccggcagcgg gacgatatgc tcgcgcgcgt cttcccacat tccttccgtc
     1201 agctccgcat aaaaatccgc cactctctcc ggcgttttct gcaagccgtc gcggtctacg
     1261 tcctcgccga tgccttccag aatcaggcgc acgccgcgcg cgattttttc caaatcaact
     1321 tttttctttt tagccaaatt ccgctcctta taagaaataa tttaacacaa gtcagaaccg
     1381 cctgccgcaa gcgcgccggt ttcatgcaga aagaaaatta gccacagatt tacacagatt
     1441 aacgcagatt tttaacagga tagacaggat attcaggata aaatagaaaa attcaatgtt
     1501 attagtttta aattatcctg ttcatcctgt ctatcccgtt tgaattttgt ttagtgactc
     1561 ggcgactttg cgttaaaagc gtccacttcc gcaaaaaatc tttttaactt ttcctcgtcg
     1621 agctttccct ccgtgcggac gctcgaacat aaatccaaac cgaacggctg aactttttcg
     1681 actgcttcgg cgacattttc cgcttttaaa ccgcccgcca gaaaaaccgg cgacttcacg
     1741 ccttcgacaa tcttgcggct aatcgtccag tcgtgcgttc tgcccgttcc gccaagctct
     1801 ttgatttcaa gcgcctggtt gccggaatcg agcagaatcg catcgacttt ttcggaaatt
     1861 tcgattgctt cccgcaaaga ttcttcgccg ccgacgtgaa cgacctgtac gagcttgacc
     1921 gcgggcagag cctttcttaa atcgtcgtat gaatcgacga agctgtcgc
//
LOCUS       DASWNE010000259         2761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_79233, whole genome shotgun sequence.
ACCESSION   DASWNE010000259 DASWNE010000000
VERSION     DASWNE010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79233"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VGB68_05385"
     CDS             complement(<1..458)
                     /locus_tag="VGB68_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048695.1"
                     /translation="MKSRLLLFLSVLLITTNAALAQNNQPAAVNEDLNRRSAESVENI
                     AKELAKISKSVTDLNTRLKTFSDTFTSNQGLRLTDKQQKLLAAFEFLNRAEQRLSNLQ
                     TLKITLAERQTSARMRLAQNEIESKPESIDRSVAVRGTITDAEEMRESRRR"
     gene            complement(508..1287)
                     /locus_tag="VGB68_05390"
     CDS             complement(508..1287)
                     /locus_tag="VGB68_05390"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0048696.1"
                     /translation="MYLALSKIDLTDVFAVWQRNSRVYVRLWKTELIAPIIEPIFTFF
                     AFGFGVGSLVASQVEGLSYLSFVGAGVLAFTVLMRAVFEMTYGAYFRMVYQSTYDAIL
                     ATPVNAESLAFGEIIWALTKGLVDCLFILPLVVAFGSATSPFTPLSIIPLLIGCLFLG
                     SFALGLTAHIFEIDHFNIFFAFFFSTIFLCGAWFPVTMLPFWMQIFAYILPTTSVIDL
                     TRACLSGNFAARHIYETVYLLVITFFLLEWALRSIRKRMVV"
     gene            complement(1287..1775)
                     /locus_tag="VGB68_05395"
     CDS             complement(1287..1775)
                     /locus_tag="VGB68_05395"
                     /inference="COORDINATES: protein motif:HMM:NF016085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEY0048697.1"
                     /translation="MTDRNALITINSRKFDDRIHRSWQARLLEETEEFYLLVGEFDSE
                     VKHSKLGVIGRGTVSYEYYWKNEWYNVFRFHEPNGELRNFYCNINRPPQLADNVLDYI
                     DLEIDILVWRDFSLEILDLDEYEESSQKFDFSDDVKYKVEESLNKLLEMIKEKAFPFD
                     YK"
     gene            complement(1772..2551)
                     /locus_tag="VGB68_05400"
     CDS             complement(1772..2551)
                     /locus_tag="VGB68_05400"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0048698.1"
                     /translation="MKTPFQESGGGKPVVLLHAFPLSRKMWQPQIDALGAAGFHVILP
                     DLRGFGENHNFADINSMEDLAKDISDLLETLKIESAVIGGLSMGGYVTFNLYRLFPEK
                     FAGLLLFDTNAAADSEEKRESRFDLIDEIEKSGAQALIDGMLPNLICDWTKENNPDLV
                     EKLKAMFAQVNPRAAIAALRGMAERKDHMNLLKDIDVPTLMIFGEHDKITNLEIAEKM
                     KSAIPGATLAKIKNAGHYSNLEQPEAFNDALVSFVKLVDIK"
     gene            complement(2556..>2761)
                     /locus_tag="VGB68_05405"
     CDS             complement(2556..>2761)
                     /locus_tag="VGB68_05405"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY0048699.1"
                     /translation="DVTITTDVLFGSPESRIVETAELMHAELIVVGSHGYNRWERLLL
                     GSVSDSVVHHAPCSVLVVRLPKT"
BASE COUNT          726 a          699 c          649 g          687 t
ORIGIN      
        1 cggcggcggc tttcgcgcat ctcttcggca tcggttattg tcccgcgcac ggcgacgctg
       61 cggtcgatgc tctccggttt cgattcaatc tcattttgcg ccagtcgcat tcgggctgac
      121 gtctgtcttt ccgccagagt aatttttaag gtttgcaaat tagagagccg ctgttccgca
      181 cgattcagaa actcgaacgc cgccagaagc ttttgctgtt tgtccgtcag ccgcagtccc
      241 tggttactgg taaaagtatc ggagaaggtt ttgagccgcg tgttcaagtc ggtcactgat
      301 tttgaaattt ttgccagttc tttagcgatg ttttcaaccg actccgccga gcggcgattc
      361 aaatcttcat tgacggcggc gggctggttg ttctgggcaa gcgccgcatt tgtcgtaatc
      421 aacaaaacgc ttagaaaaag aagcagtctc gatttcattt tcaattctcc ttttgaccga
      481 aacaatttaa cacgcttttt ctgcaaacta aaccaccatt cttttcctga tgctgcgcaa
      541 agcccattcg agcagaaaaa acgtgatgac caacagataa accgtttcgt aaatgtgccg
      601 cgcagcgaaa ttgccgctca ggcaggcgcg cgttaaatca atcacgctcg tggtcggcaa
      661 aatatacgcg aagatttgca tccagaaggg aagcatcgtc accggaaacc aagcgccgca
      721 taaaaaaatc gtcgagaaaa agaaagcgaa aaagatattg aaatggtcaa tctcgaaaat
      781 atgcgccgtc agccccagcg cgaagctgcc cagaaacagg cagccgatga gcaggggaat
      841 aatggacaaa ggcgtgaacg gcgaagtcgc cgaaccgaac gcgacgacca aaggcaaaat
      901 gaaaagacaa tcgaccaagc ctttggtcag cgcccagatt atttcgccga aggcgagcga
      961 ttcggcgttg acgggcgtgg cgagaatagc gtcgtaagtc gattgataaa ccatccgaaa
     1021 atacgcgccg taagtcatct cgaaaaccgc gcgcatcaaa accgtaaacg ccagaacgcc
     1081 ggctccgaca aacgacaggt agctcaagcc ttcgacctgc gaagcgacca gcgagccgac
     1141 gccgaaaccg aaggcgaaaa aagtaaatat cggctcgata atcggcgcga taagctcggt
     1201 tttccagagc cgcacgtaaa cgcgcgagtt tcgctgccag accgcaaaaa cgtcggtcaa
     1261 atcaattttg gaaagcgcca gatacattat ttataatcaa agggaaacgc tttttctttt
     1321 atcatttcca gtaatttgtt caagctttct tcgactttgt attttacatc atcggaaaaa
     1381 tcaaactttt gcgagctttc ttcatattcg tccaaatcca gaatctcaag gctgaaatct
     1441 cgccagacga gaatgtcgat ttccaaatct atataatcca atacattatc tgcaagctgc
     1501 ggcgggcgat tgatgttgca gtaaaaattt cttaattcgc cgttcggttc gtgaaaccga
     1561 aatacgttgt accattcgtt tttccagtaa tattcatagc tgacggttcc gcgtccgata
     1621 acgcctaatt ttgaatgttt gacctccgaa tcaaactcgc cgaccagcag ataaaattct
     1681 tcggtttctt ccaaaagcct ggcttgccag ctgcggtgga ttctgtcgtc gaattttcga
     1741 gaattaatcg tgattaaagc gtttcggtct gtcatttaat atcaaccagc ttgacgaaag
     1801 aaaccagcgc gtcgttaaac gcttcgggct gttccagatt tgaataatgt ccggcatttt
     1861 tgattttagc cagcgttgcg ccgggaattg cggatttcat tttttcggca atttccagat
     1921 tagtaatctt gtcgtgctcg ccgaaaatca taagcgtcgg aacatcgatg tcttttaaaa
     1981 gattcatatg gtcttttcgt tccgccattc cgcgaagcgc cgcaattgcc gcccgcggat
     2041 ttacctgtgc aaacatcgcc ttgagctttt cgaccaaatc cgggttgttt tcctttgtcc
     2101 aatcgcaaat caaattcggg agcattccgt ctatcagcgc ctgcgcgccg gatttttcga
     2161 tttcgtcaat caaatcaaaa cgcgattcgc gtttttcttc ggaatccgcc gccgcgttgg
     2221 tatcaaacaa aagcagaccg gcgaattttt cgggaaacag gcgatacaga ttaaacgtga
     2281 cgtaaccgcc catcgaaagc ccgccgataa cggcgctttc tatttttaga gtctccagca
     2341 aatcggagat gtctttcgcc aaatcttcca tcgaattgat gtcggcgaaa ttatggtttt
     2401 cgccaaagcc gcgcaaatcc ggcaaaatta cgtgaaaacc ggcagcgccg agcgcgtcga
     2461 tttgcggctg ccacattttt cgggaaagcg gaaaagcgtg cagcaaaacg acgggttttc
     2521 cgccgccgct ttcttgaaag ggagttttca taatcttatg ttttgggcag ccggacaacc
     2581 agaaccgaac acggcgcgtg atgcaccacc gaatcggaaa cggagcctaa aagcagccgc
     2641 tcccatcgat tatatccgtg cgagccgacg acgattaatt cggcgtgcat cagttcggcg
     2701 gtttcgacga tgcggctttc cggcgagccg aataaaacgt ccgtcgtaat cgtcacgtct
     2761 c
//
LOCUS       DASWNE010000260         3946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_395050, whole genome shotgun sequence.
ACCESSION   DASWNE010000260 DASWNE010000000
VERSION     DASWNE010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395050"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VGB68_05410"
     CDS             complement(<1..356)
                     /locus_tag="VGB68_05410"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY0048700.1"
                     /translation="MLLVQAVSAQNSTGFLQGIVTDENRAIIPEAKIVLRDEKDGRRE
                     IVSGIDGSFSFKDLAFGKYILTVEKDGFTAVEREIVLSAPEQNSDLTLAPAAVSESVT
                     IVLDSAEMAVESTLKLP"
     gene            771..2759
                     /locus_tag="VGB68_05415"
     CDS             771..2759
                     /locus_tag="VGB68_05415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEY0048701.1"
                     /translation="MEKLNEYLQTLLSTCSYELHLEPNKQPFVVAANGATDIANAPLL
                     GTQISTMIFPLIPVNVRQELPSRPEVEFTHTHNLGDFSFLVRKSPSGFNVTIRPVLSS
                     TIASALPELEAKPSSPIFNQTIKEPISYPTESNHSVFNQETVSPAPTPAPFSKAEPFS
                     FESSSAEREQISDFVPESSFAPEFVFEEPPVLSENDAAQPQIEIVPVNDPEFITTFSD
                     SSTYEPPGRRDDFYPQTEAYEPPQNNFTQQQTSFSQPTFQAEQTFEQAQPAQAQPQFS
                     APPVQQFYAPPPESPSAAFAAASANPQAKAQMDAMFQKMAERGASDLHLSVSMPPMIR
                     KDGRMQALEPGEPALTPVMMRELLNSIMPAKNQEEFAVRRDSDFAYEIPGLARFRANI
                     FMDRKGMGGVFRIIPTKILTAEQLGLSPAIMHLCELSKGLVVVTGPTGSGKSTTLCAM
                     IDSINKRREDHIITIEDPIEFTHENQKCLVNQREVHNHTDSFKDALRAALREDPDILL
                     VGEMRDLETISIAIETAETGHLVFGTLHTTTAASTIDRIIDQFPADRQQQIRVMLSES
                     LKGVIAQTLLPKKGGGRVAALEILIVTPAISNLIREGKTYQIPSAMQTGKQHGMVMLN
                     DALFGLVQKGLVEPVDAFVKAVDKANFESLLQRNGFKL"
     gene            complement(2968..3648)
                     /locus_tag="VGB68_05420"
     CDS             complement(2968..3648)
                     /locus_tag="VGB68_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEY0048702.1"
                     /translation="MKQLNRYYRCAKISERKIRQIIKYFALDLTANKTAQLSDVTHQS
                     INRIYLKIRQRIAEEAERASPFTACEIEVDESYFGARRVRGKRGRGAGNKTIVFGIYK
                     RNGKVYTEIVPNAAKKTLQNIIRGKVSLDSVIHSDGWRGYNGLVDVGYSKHLRINHSD
                     DVFAIGDVHINGIESFWSYAKRRLQKFNGVPTKTFYLHLKECEYRFNHRNENLQLLLL
                     KLLERFPL"
     gene            complement(3669..>3946)
                     /locus_tag="VGB68_05425"
     CDS             complement(3669..>3946)
                     /locus_tag="VGB68_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048703.1"
                     /translation="EQIKAPCFAKSDHDYSSFSRYYPSFHNATEPSSWSFIYKAFIEN
                     IYPLLKKNSIIARRKRYVPLSNYISIAEAEKNLKVTKKEGSMVANKQ"
BASE COUNT         1048 a         1002 c          893 g         1003 t
ORIGIN      
        1 ggcaatttta aagtcgattc gaccgccatt tccgcgctgt ccaaaacgat tgtcaccgat
       61 tcgctgaccg ccgccggcgc aagcgtcaaa tccgaattct gctccggcgc cgacaggacg
      121 atttctcgct cgacggcggt aaaaccgtct ttctcgaccg tcaaaatata ttttccaaaa
      181 gctaaatctt taaacgagaa actgccgtcg atgccggaaa caatttcacg cctgccgtct
      241 ttttcgtcgc gcagaacgat tttggcttcg ggaataatcg cccggttttc gtcggtgacg
      301 atgccctgca aaaagccggt cgaattctgt gccgaaaccg cctgaacgag caacatcgca
      361 gccaaaagaa aaaacaatcc aaagttaaat aaatgtcgat gtcttctcga tgccgtcata
      421 tcaattagaa ctcctttcac aaactggaaa tcatccgaaa ccgctttccg gcagaactta
      481 tgtcccgctc tcgccctttg aaaaacattt aaaatctccg gcgcgataaa ctaatcggcg
      541 gcgttcattc agaaacaaaa taaattttat gaatgtgaaa ttgaaaatcg ttttcaataa
      601 gaatcgaata taaaataacg agttgagcgt gtcaaacgca aaaagttgat tcgtataagc
      661 acaaagttaa tgacaaacat tgttttgata tgttaatcta aatttcgagt cttttccgac
      721 tcgtcactca gcacaccgga tttcccaaat cgacaaaccg aaatttcaac atggaaaaat
      781 tgaacgaata cctgcaaacg cttctttcta cttgtagtta cgaattgcat ctcgaaccga
      841 ataaacaacc ttttgtcgtc gccgcaaacg gcgcgacgga catcgccaac gcgccgcttc
      901 taggaactca aatcagcacg atgatttttc cgctgattcc ggtaaacgtg cggcaggagc
      961 tgccgagccg acctgaagtc gaatttacgc acacccacaa tctcggcgat ttcagttttc
     1021 tggtcagaaa atctccgtcg ggctttaatg tcacgattcg tccggttctt tcttcgacca
     1081 tcgcttccgc gctgccggaa ctcgaagcta aaccgtcttc gccgatattt aatcaaacga
     1141 taaaagagcc gatttcttat ccgaccgaat cgaatcattc cgtttttaat caggaaacgg
     1201 tttcgcccgc gccgacgccc gcgccttttt ccaaagccga gcccttttca tttgaaagct
     1261 cttccgccga acgcgaacaa atttccgact ttgtgcccga atcgtctttc gcgcccgagt
     1321 ttgtcttcga agaacctcca gtcttatcgg aaaatgacgc tgcgcagcct cagatagaaa
     1381 tcgttccggt taacgacccg gaatttatca cgactttttc cgacagttcg acttacgaac
     1441 cgcccggacg ccgcgacgat ttttacccgc aaaccgaagc ttacgagccg ccgcaaaata
     1501 atttcacgca gcagcagacg tccttttcac agccgacttt tcaagccgaa caaacattcg
     1561 agcaagctca accggctcaa gcgcagccgc agtttagcgc gccgccggtg cagcagtttt
     1621 acgctccgcc gccggaatcg ccgtccgccg cttttgccgc cgcttcagcc aatccgcagg
     1681 caaaagcgca gatggacgct atgtttcaaa aaatggcgga acgcggcgcg tcggatttgc
     1741 atctgtcggt cagtatgccg ccgatgattc gcaaagacgg caggatgcag gctctagagc
     1801 cgggcgagcc agccctgacg cccgttatga tgcgcgagct tttaaactcg attatgcccg
     1861 cgaaaaatca ggaagagttt gccgtccggc gcgattcgga ttttgcttat gagattcccg
     1921 gcttggcacg ttttcgcgcc aatattttta tggacagaaa aggaatgggc ggcgttttcc
     1981 gcatcattcc gacgaaaatt ttaaccgccg aacagctcgg actttcgccc gcgattatgc
     2041 acctgtgcga actgtcgaaa ggcttggtcg tcgtcacggg tccgaccgga agcggaaaat
     2101 ccaccacgct ctgcgcgatg attgacagca tcaacaaacg gcgcgaagac cacattatca
     2161 ccatcgaaga cccgattgaa ttcacgcacg aaaatcaaaa atgtctggtc aaccagcgcg
     2221 aggtgcacaa tcacaccgac agttttaaag acgcgctgcg cgccgcgttg cgcgaagacc
     2281 cggacattct gctcgtcggc gaaatgcgcg atttggaaac aatttcgatt gcgattgaaa
     2341 cggcggaaac cgggcacctc gtcttcggaa cgctgcacac gacgaccgcc gcttcgacga
     2401 ttgaccgaat catcgaccaa tttcccgccg accgccagca gcagattcgc gtgatgcttt
     2461 cggaatcgct caaaggcgtt atcgcgcaga cgctgctgcc gaaaaaaggc ggcgggcgcg
     2521 ttgcggcgct ggaaattttg attgtcactc cggcgatttc gaatctgatt cgcgaaggca
     2581 aaacctacca gattccttcg gcgatgcaga ccggcaaaca gcacggaatg gtaatgctga
     2641 acgacgcttt gttcggattg gtgcagaaag gcttggtcga gccggtcgat gcttttgtca
     2701 aagccgtcga caaggcgaat tttgaatcgc ttttgcagcg aaacggtttc aagctttaaa
     2761 cgagttagag acgaaaagtg aagggcgagg agaaaaaacc ggttttttct cctcgccttt
     2821 tgttttttcg agtcaagaaa aacagtttct ctttttgcag gaatttgcta agacgcgagc
     2881 cgtaaatcaa acaaataacg catcacgata agtaaaaaag tccgtttgct tttcttaaaa
     2941 attaagggtc tatggtagca aaagtgttta gagcggaaac cgctcaagca attttaacag
     3001 caaaagctgt aagttttcat ttcgatgatt aaagcgatat tcacattctt tcaagtgcaa
     3061 atagaaagtt tttgtcggca caccgttgaa cttttgcaga cggcgtttgg catacgacca
     3121 gaaactttca atgccgttaa tgtgaacgtc gccaatggca aaaacatcgt ccgaatgatt
     3181 gattcgcaga tgcttcgagt atccgacatc aaccagtcca ttgtaaccgc gccagccatc
     3241 ggaatgaatc acactgtcaa gactgacttt gccgcgaata atattttgca gcgttttctt
     3301 cgccgcgttc ggcacaatct cggtataaac tttcccgttg cgtttgtaaa ttccgaagac
     3361 gattgtttta ttgcccgctc cgcgtccacg tttgccgcgc actcggcgag caccgaagta
     3421 agattcgtca acttcgattt cgcaggcggt gaaaggcgaa gcgcgttcgg cttcttcggc
     3481 aatgcgttgt ctgattttca aatagattcg attgatactc tgatgagtta catcgctcaa
     3541 ctgagcagtt ttattagctg tcaaatcaag cgcaaaatac ttgatgattt gacgaatttt
     3601 ccgctcagaa attttagcgc accgataata tcggtttagt tgtttcatta cagtagctta
     3661 caagtttatc attgcttgtt tgctaccata gacccttcct ttttagtgac tttcagattt
     3721 ttttccgctt cggcaatcga aatatagttc gacaatggga catatcgttt tctcctggca
     3781 attattgaat tcttcttaag cagaggatag atattttcaa taaaagcttt gtatataaat
     3841 gaccacgatg agggttctgt tgcgttatga aaagagggat aatagcgact gaaagatgag
     3901 taatcgtggt cgcttttggc aaagcagggt gctttaatct gttcct
//
LOCUS       DASWNE010000261         1746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_43467, whole genome shotgun sequence.
ACCESSION   DASWNE010000261 DASWNE010000000
VERSION     DASWNE010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43467"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..496
                     /locus_tag="VGB68_05430"
     CDS             11..496
                     /locus_tag="VGB68_05430"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0048704.1"
                     /translation="MEKFRFRHLAPSDYSVLLEWLSQKHVAEWWDDGDDTIEKVAAHY
                     GAADETRRFLLLDGEKPIGYFQYYFDGEETIGIDQFIGDETYLNRGVGEKTIKMFVRS
                     IAEKHNPAFVILDPSPENARAVRCYEKVGFRHYETRQLDNGETAYMMRLKIRPPGGKG
                     R"
     gene            503..1603
                     /gene="wecB"
                     /locus_tag="VGB68_05435"
     CDS             503..1603
                     /gene="wecB"
                     /locus_tag="VGB68_05435"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEY0048705.1"
                     /translation="MLKILNIVGARPNFMKIAPIVREMRRRENDFLPLIVHTGQHYDA
                     AMSDSFFADLDIPAPDFHLEVGSASHAVQTARVMTAFEPVVVEEKPDWVLVVGDVNST
                     IACALVCSKLGIKVAHVEAGLRSRDRTMPEEINRILTDAISDLLLTTSPDADENLKRE
                     GIAEEKIKFVGNVMIDSLFYNLKKAETSKVREDLNLNAKDYAVLTLHRPSNVVEKDVF
                     AGLLEALVPISEKLPVIFPVHPRTKSRIDEFGFAKAVENSNIKLIEPLGYLDFMRLYS
                     GARLVLTDSGGLQEETTALGIPCLTLRENTERPITIELGTNNLVGTNPEKIKQTAFGI
                     LENRSNSKDKKIPPLWDGRAAERICNQLITDN"
BASE COUNT          449 a          454 c          438 g          405 t
ORIGIN      
        1 tcgtttataa atggaaaagt tcaggttcag gcatctcgcg ccgtccgatt attcggtgct
       61 gctcgaatgg ctttcccaaa aacacgtcgc cgagtggtgg gacgacggcg acgacacgat
      121 cgaaaaagtc gccgcgcatt acggcgcggc tgacgaaacg cggcgatttc tcttgctgga
      181 cggcgaaaag ccgatcggct attttcaata ttatttcgac ggcgaggaga caatcggaat
      241 cgaccagttt atcggcgatg aaacatatct gaatcgcggc gtcggcgaaa aaacgattaa
      301 aatgtttgtc cggtcgatag ccgaaaaaca taatcccgcc tttgtcatcc tcgacccgtc
      361 gccggaaaac gccagagccg tcagatgtta tgaaaaagtc ggtttccgtc attacgaaac
      421 cagacaactc gataacggag agacggcgta tatgatgcgc cttaaaattc gtccgccggg
      481 cgggaaaggg cgataaagat aaatgttaaa aatcctgaac atcgtcggcg cgcgtccgaa
      541 ttttatgaaa atcgcgccga ttgtccgcga gatgcgccgg cgcgaaaacg attttctgcc
      601 gcttatcgtg cataccgggc agcattacga cgcggcgatg tcggattctt ttttcgcgga
      661 tttggatatt cccgcgcctg attttcatct cgaagtcggt tcggcttcgc acgccgttca
      721 aaccgcgcgc gtgatgacgg ctttcgagcc ggtcgtcgtc gaggaaaagc ccgattgggt
      781 tctggtcgtc ggcgacgtta actcgaccat cgcctgcgct ctggtttgct cgaagctcgg
      841 aatcaaagtc gcgcacgtcg aagcgggctt gcgctcgcgc gatcgcacga tgcccgaaga
      901 aatcaaccga atcttaaccg acgcgatttc cgatttgctt ttgacgactt cgccggacgc
      961 cgacgaaaat ctcaaacgcg aaggcatcgc cgaggaaaaa ataaaatttg tcggcaacgt
     1021 gatgattgat tcgctttttt acaatctgaa aaaagccgaa acctcgaaag tcagagaaga
     1081 tttgaactta aacgcgaagg attacgccgt tttgacgctg catcgcccgt cgaatgttgt
     1141 cgagaaagac gttttcgcgg gcttgctcga agcgctcgtc ccgataagcg aaaagcttcc
     1201 ggttattttt cccgttcacc cgcgcaccaa atccagaatc gacgagttcg gctttgcgaa
     1261 agcggtcgaa aactcgaata tcaaacttat cgagccgctc ggctatctcg attttatgcg
     1321 gctttacagc ggcgcgcggc tggttttaac cgattccggc ggcttgcagg aagaaacgac
     1381 ggcgctcgga attccctgtc tgaccctgcg cgaaaatacg gaaagaccaa tcaccatcga
     1441 gctcggaaca aacaatctgg tcggcaccaa tcccgaaaaa atcaaacaaa ccgctttcgg
     1501 gattctggaa aaccgttcaa actcaaaaga caaaaaaatt ccgccgctct gggacgggcg
     1561 agcggcggaa cgaatctgta atcaactgat aactgataac tgataactga tagttcgttc
     1621 tttcactatc agttatcagt tatcagttat cagttctcca ctattattcg ctttttccca
     1681 tcggataacc ggcatctttc caggcttttg tgccgccgac taaagcaaac gcgtttccga
     1741 tacctt
//
LOCUS       DASWNE010000262         1694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_43478, whole genome shotgun sequence.
ACCESSION   DASWNE010000262 DASWNE010000000
VERSION     DASWNE010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43478"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..591)
                     /gene="ruvX"
                     /locus_tag="VGB68_05440"
     CDS             complement(100..591)
                     /gene="ruvX"
                     /locus_tag="VGB68_05440"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEY0048706.1"
                     /translation="MQEKETINPPDFSEISHGRIVALDLGTRWVGVAVCDELQVTVRP
                     VGVVQRRSWKELLRKISAFIEEFDAVALVLGLPYNFDGSESEMSLEARRLHRNFTLSL
                     KIPVYLQDERLTSKAADKYLREQRLSEKKIRQQIDSNAAAFILNDFLALKHPTDQRNI
                     QTG"
     gene            694..>1694
                     /locus_tag="VGB68_05445"
     CDS             694..>1694
                     /locus_tag="VGB68_05445"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY0048707.1"
                     /translation="MSFRNKIFLVLGSAAIAFYAVLGSCAVTGSFLGVNAQQPINDAG
                     AQMRIFESVLQHIQNDYVDEPNLERVRAGALRGLAYGLDPYSSYLTVEQVRDYQAKKA
                     ANQIGIGAEFSQVSGYLYVISVTKGSPAEKAGLKSGDVIEYIENKATRDISLYDARQL
                     LFGEAGSKVNVRVLRAGAKPQTIAVTRGIFKVPEAEARVETGKIGVIKVYSLDEGEAS
                     DIRSQVQNLQKQGVQKIVLDLRGLATGNLNEAVGVANIFIKDGVLAQIIGRENKVTKT
                     FTADAKNFLFDGKVVALIDLGTSGVGEVVASAILDRQRGEVVGERSFGAGTEQQLIPL
                     "
BASE COUNT          415 a          398 c          452 g          429 t
ORIGIN      
        1 cagaatattt tgaaacgatg aatgatgaac gatgaatgat gaacgatgaa tgatgaattc
       61 tttattccgc gttcatcgtt catcgttcat cgtttcgatt tatcctgttt gaatatttct
      121 ctggtcggtc ggatgtttta aggcaagaaa gtcattcaag ataaacgccg ccgcgttgct
      181 gtcgatttgc tgtctgattt ttttttcgct gagtcgctgt tcgcgcaggt atttatccgc
      241 cgccttcgag gtcaggcgct cgtcctgcaa gtagaccgga attttcagcg agagcgtgaa
      301 attacggtgc aggcggcgcg cttccaggct catttcgctt tcgctgccgt cgaaattgta
      361 cggcaaacct aaaaccagtg cgaccgcatc aaactcctcg ataaaagcag agattttccg
      421 caagagttct ttccagctgc gacgttgtac gacaccgacg ggacgcaccg taacctgcaa
      481 ttcatcgcag acggcgacgc cgacccatct ggtgcctaaa tcgagggcga cgatgcgccc
      541 gtgagaaatc tcggaaaagt ccggcggatt aatagtttct ttttcttgca cgtaaataat
      601 tgataattgt taacggataa tggataattg cgtcaacata attggtatta tccttcaggc
      661 gcgtatcaat tatccactaa tagtgaggtt ttaatgtctt ttcgcaataa aatttttctt
      721 gttttgggtt cggcggcaat cgctttttat gcggttttag gcagctgcgc ggtgacggga
      781 agttttctcg gcgttaatgc tcaacagccg ataaacgatg ccggagcgca aatgcgcatt
      841 tttgaaagcg ttcttcagca tattcaaaac gattatgttg acgaaccgaa tctggaaaga
      901 gttcgtgccg gcgcgctgcg cggtctggct tacgggctcg acccgtattc gtcttatctg
      961 acggttgagc aggtcagaga ttatcaagcc aaaaaggcag ccaatcaaat cggcatcggc
     1021 gcggaatttt cgcaggtttc cggttatctc tacgtgattt cggtgacaaa aggatcgccc
     1081 gccgaaaaag ccggattaaa atcgggcgac gtgattgaat acatcgaaaa taaagcgacg
     1141 cgcgacattt cgctttacga cgcgcgccag cttcttttcg gcgaagccgg ctcgaaggtc
     1201 aacgtgcgcg ttttgcgcgc gggcgccaag ccgcagacca ttgccgtgac gcgtggaata
     1261 tttaaagttc ccgaagccga agccagagtc gaaacgggca aaatcggcgt cataaaagtt
     1321 tatagcctcg acgaaggcga agcgtcggac attcgcagtc aggtgcagaa tctgcaaaaa
     1381 cagggcgtgc agaaaatcgt tctcgatttg cgcggcttgg cgacgggcaa tttaaacgaa
     1441 gctgtcgggg tcgctaatat tttcatcaaa gacggcgttc tggcgcaaat catcggacgc
     1501 gaaaacaagg tcacaaaaac atttaccgcc gatgcgaaaa actttttgtt tgacgggaaa
     1561 gtcgtggcgt tgattgattt gggcacttcg ggcgtcggcg aagtcgtcgc ttcggcgatt
     1621 ctggaccgcc agcgcggcga agtcgtcggc gaacgcagct tcggcgcggg aaccgagcag
     1681 cagcttatcc cgct
//
LOCUS       DASWNE010000263         2260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_360424, whole genome shotgun sequence.
ACCESSION   DASWNE010000263 DASWNE010000000
VERSION     DASWNE010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360424"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VGB68_05450"
     CDS             <1..198
                     /locus_tag="VGB68_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048708.1"
                     /translation="HGHRREAINLFFNNLRGAKSAAQIGKMLFRLAVPQKIFQWNRKR
                     KRQKAIEKYGNLNEEMMNAKR"
     gene            253..1077
                     /locus_tag="VGB68_05455"
     CDS             253..1077
                     /locus_tag="VGB68_05455"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0048709.1"
                     /translation="MHETQITKIKPESSRLNLNLKDLWIYRELLYFMTWRDIKVRYKQ
                     TAIGVLWAILQPVLTTAIFTVIFTQFARFESLEIPYPLFALSGLLLWLFVNNSINMSS
                     NSLVGNANLVTKVYFPRLIVPVSATLAGLLDLALGFLILLGLMIYYGVNPTWQILLAP
                     VFVLQTILLTLSFGTLFSALNVRFRDVKFALPFALQIWLFLSPVFYPLGIFSGKRQFL
                     FAFNPMSGILEGFRAALFGTAFDWTIIGISFVLTLVLMFVSIFVFKRMEDDFADLI"
     gene            1209..1448
                     /locus_tag="VGB68_05460"
     CDS             1209..1448
                     /locus_tag="VGB68_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048710.1"
                     /translation="MFIHGAGKDYMELTRQPARYRHAFNTVINPFYVFVCALTLLYVI
                     AGEMHWFFLAAAGFFAFILFVVDGSAWIEKRSKKD"
     gene            1452..>2260
                     /locus_tag="VGB68_05465"
     CDS             1452..>2260
                     /locus_tag="VGB68_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0048711.1"
                     /translation="MEVIRAENLSKLYYLGGGTRHNSLRDTLMSFVRKPSLSREKQEL
                     WALKDLNFTVDDGETLGIIGHNGAGKSTLLKILSRITKPTRGTAEIRGRVASLLEVGT
                     GFHSELSGRENMYLNAAILGMKRGELERKFDEIVAFAEIEKFIDTPVKFYSSGMYMRL
                     AFAVAAHLEPEILIVDEVLAVGDIAFQKKCLNKMRDVRQEGRTVLFVSHNLQAVSRLC
                     RRVIWLERGKVREDGEAQQIVSRYLHAQTNSNSEKIWDNPANMPGNEITRLL"
BASE COUNT          511 a          508 c          583 g          658 t
ORIGIN      
        1 cacgggcacc ggcgcgaagc gataaattta tttttcaata atttgcgcgg agccaaatcc
       61 gccgcgcaaa tcgggaagat gctttttcgc cttgccgttc cgcagaagat ttttcaatgg
      121 aacagaaagc ggaagcggca aaaagcgatt gagaaatacg ggaatttgaa tgaagaaatg
      181 atgaacgcga aacgataaac gatgaattgc gcgttccgcg ttcatcattc atcgttccgc
      241 gttttaatga aaatgcacga gacgcagatt accaaaataa aacccgaaag ctcgcggctc
      301 aatctgaatt taaaagacct ttggatttac cgcgaactgc tttattttat gacgtggcgc
      361 gacataaaag ttcgctacaa gcagacggca atcggtgttc tgtgggcgat tttgcagccg
      421 gttttgacga ccgctatttt taccgtgatt ttcacgcagt ttgcgcgctt cgaatcgctg
      481 gaaattcctt acccgctttt tgctctgagc ggacttctgc tctggctttt cgtcaataac
      541 tcgataaata tgtcgagcaa cagtttggtc ggcaatgcca atctggtcac gaaagtttat
      601 tttccgcgcc tgattgtgcc ggtttccgcg acgcttgccg ggctgctcga tttggcgctg
      661 ggttttttga ttctgctcgg cttgatgatt tattacggcg tcaacccgac gtggcagatt
      721 ttgctcgcgc cggtttttgt tttgcagacg attttgctga ctttgagttt cgggacgctg
      781 ttttcggcgt taaacgtgcg ttttcgcgac gtcaaattcg cgctcccgtt cgctttgcaa
      841 atctggctgt ttctttcgcc ggtcttttat ccactcggca ttttttccgg gaaaagacaa
      901 tttttatttg cctttaatcc gatgtccgga attctggaag gctttcgcgc ggcgctgttc
      961 ggaaccgcgt tcgattggac gataatcggc atttcatttg ttctgacgct cgttttaatg
     1021 ttcgtttcga ttttcgtttt caaacgaatg gaagacgatt ttgcggattt aatttaacac
     1081 ggatgaatta tcgaaagtat ttcagtcagg ctttgctttt tagcggatta attttcggca
     1141 ctggcttttt aaacttttgg ggattcgact tttacgtctg cgtgttgtcg gctctgtgtt
     1201 ttttatttat gtttattcac ggcgcgggca aagattatat ggagttaacg cggcagccgg
     1261 cgcgctatcg tcacgctttt aatacggtta tcaatccgtt ttacgttttt gtctgcgcgc
     1321 tcacgcttct ctatgtgatt gccggcgaaa tgcattggtt ttttttggcg gctgccggtt
     1381 ttttcgcgtt tatccttttt gttgtcgacg gctcggcttg gatagagaaa agaagtaaaa
     1441 aagattagct gatggaagtt atcagagcgg aaaatttatc aaagctttat tacctcggcg
     1501 gcggaacgcg gcataattcg ctgcgcgata cgttgatgag ctttgtccgc aaaccttcgc
     1561 tttccagaga aaagcaggag ctgtgggctt tgaaggattt aaattttacg gtcgacgacg
     1621 gcgaaacttt aggcattatc gggcacaacg gcgcgggcaa atcgaccttg ctgaaaattc
     1681 tttcgcgcat cacgaaaccg acgcgcggga cggcggaaat ccgcgggcgc gtcgcttctc
     1741 tgctggaagt cgggacgggt tttcacagcg agctttcggg tcgcgaaaat atgtatttaa
     1801 acgccgcaat tctcgggatg aagcgcggcg agctggagcg caaatttgac gaaatcgtcg
     1861 ctttcgccga aatcgagaaa tttatcgaca cgcccgtcaa attttattcc agcggtatgt
     1921 atatgcggct cgccttcgcc gtcgccgcgc atctcgaacc ggaaattctg attgtcgacg
     1981 aagttttagc cgtcggcgat atcgcttttc agaaaaagtg tctcaacaaa atgcgcgacg
     2041 tccggcagga aggcagaacg gtcttgttcg tttctcataa tttacaggct gtctcgcggc
     2101 tttgccggcg cgtcatctgg ctggagcgcg gaaaagtccg cgaagacggc gaagcgcagc
     2161 aaattgtcag ccggtatctg cacgctcaga cgaattccaa cagcgaaaaa atctgggata
     2221 atccggcgaa tatgccggga aacgaaatta cgagattgct
//
LOCUS       DASWNE010000264         3679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_256234, whole genome shotgun sequence.
ACCESSION   DASWNE010000264 DASWNE010000000
VERSION     DASWNE010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256234"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /gene="rplC"
                     /locus_tag="VGB68_05470"
     CDS             <1..412
                     /gene="rplC"
                     /locus_tag="VGB68_05470"
                     /inference="COORDINATES: protein motif:HMM:TIGR03625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEY0048712.1"
                     /translation="TPPTRILKEIRLDGDADVNVGDQVKADLFADGDMIDVIGKSKGR
                     GFAGTIKRHKFHRGPESHGSMSVRAPGSIGQSAYPSRVIKGTRSSGHMGDARVTVKGL
                     TVARVDIENNLIMIRGAVPGANGSLVVVRKASKK"
     gene            520..1260
                     /gene="rplD"
                     /locus_tag="VGB68_05475"
     CDS             520..1260
                     /gene="rplD"
                     /locus_tag="VGB68_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEY0048713.1"
                     /translation="MPTAKVRNLSNNEVGDIELSDAVFNAELNESLIHEAVRNYLANR
                     RQGTSATKTRGNVSGSGRKLWKQKGTGRARIASLRSPLWKGGGNVHGPQPRDWSYQLP
                     KKMRRGALRSALSERLREGNLIIIDDFASVGAKTREFIGVLETLGLVDKKKTTKTLIV
                     DSLDNANLIRSSRNVQKTKVTNSYGLNIYDLLYHERLLVSKTAIEELSQMLDPTRERG
                     SANSNGATAVETSATDRAGENTEEAANE"
     gene            1253..1579
                     /gene="rplW"
                     /locus_tag="VGB68_05480"
     CDS             1253..1579
                     /gene="rplW"
                     /locus_tag="VGB68_05480"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEY0048714.1"
                     /translation="MSKVLNMNVWDILKTPVITEKSVLLKEQSSEEGEAQVLSFKVDR
                     KASKNDIRKAVEEIFSVKVSKIRTVNYEGKIKKRGRYEGRRASWKKAYVTLQKGEPIV
                     DYAEAI"
     gene            1661..2491
                     /gene="rplB"
                     /locus_tag="VGB68_05485"
     CDS             1661..2491
                     /gene="rplB"
                     /locus_tag="VGB68_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEY0048715.1"
                     /translation="MGIKKLKPTSPARRYQTYLTRDELTPNAKPEKSLLEPKTRISGR
                     NNDGRITVRRRGGGHKRFYRIVDFKRNKTGIPGRVTQIEYDPNRSAHIALITYLDGEK
                     RYILAPVGITVGSTVMSGAEADILPGNALPIQNIPLGTQIHNIELRPGKGGQMARSAG
                     SFAQLVAKEGDFAQLRMPSGEVRRVPVVCMATVGQVGNTENENVSLGKAGRSRWKGLR
                     PKVRGVAMNPVDHPHGGGEGKTSGGRHPVTPWGQPTRGYKTRNNKRTQKFIVRDRRSK
                     "
     gene            2571..2861
                     /gene="rpsS"
                     /locus_tag="VGB68_05490"
     CDS             2571..2861
                     /gene="rpsS"
                     /locus_tag="VGB68_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEY0048716.1"
                     /translation="MPRSVKKGPFIDLSVQRILRKISESGSKPQAIKTWSRRSTITPE
                     MVGLTFGVHNGKRHVPVYVTENMVGHKLGEFSPTRTFKGHPGTKAEKMAKRK"
     gene            2931..>3679
                     /gene="rplV"
                     /locus_tag="VGB68_05495"
     CDS             2931..>3679
                     /gene="rplV"
                     /locus_tag="VGB68_05495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEY0048717.1"
                     /translation="MEAIAKTKHLKGSPRKARLVIDMIRGVNVSRALAILKFTDKRAA
                     DPIAKTLLSAIANATFKAEKQNIAIDPDELFVKSCFVDMGAHKNRRRMRPAPQGRAYR
                     EQRHYCHITIEVSSDKPEEGKVRNAKITGEEETKKSTSKRAAIKKTDAGERTAKASAQ
                     KADEVTESRAAAHEANVEEVETTATTATTATEQTAGETTTAATEETSPKATESSKAVT
                     EEATEGDAAASDAKDTATQFPAELNETKRAD"
BASE COUNT         1070 a          899 c         1011 g          699 t
ORIGIN      
        1 cacgccgccg acgcgtatct tgaaagaaat tcgtctcgac ggcgacgccg acgtcaatgt
       61 cggcgaccag gtgaaagccg atttatttgc cgacggcgac atgatcgacg ttatcggcaa
      121 atcgaaaggt cgcggtttcg cgggcacgat taagcgtcac aaattccatc gcggtcccga
      181 atcgcacggc tcgatgtcgg tgcgcgcgcc gggttcaatc ggtcaatcgg catatccttc
      241 gcgcgttatt aaaggaacgc gctcatccgg tcatatgggc gatgcgcgcg tgacggtaaa
      301 gggcttgacc gtggcgagag tcgatattga aaacaatctg attatgattc gcggcgctgt
      361 gccgggagcc aacggaagtc tggttgtcgt tagaaaagca agcaaaaagt aattttagat
      421 ttatgatttc agattttaga ttgaaatcgg taattgattt taattccgaa tccgatcgcc
      481 catcgaaaat ctaaaatcga aagtaaacgg agtgagatta tgcctaccgc aaaggttcgc
      541 aatttaagta ataatgaagt cggcgacatt gaattgtccg atgccgtgtt taacgccgag
      601 ctgaatgaat ctctgattca cgaagcggtc agaaactatc tggcaaatcg ccgacaagga
      661 acgtcggcga caaaaactcg cggcaacgtt tccggttcgg gtcgcaagct ctggaaacag
      721 aagggaacgg gtcgcgcgcg tatcgcgtcg cttcgctcgc ccttgtggaa aggcggcggc
      781 aacgttcacg gaccgcagcc gcgcgattgg tcttatcaat taccgaagaa aatgcgtcgc
      841 ggcgctctgc gctcggctct gtcggaaaga ctgcgcgaag gcaatctgat tatcatcgac
      901 gatttcgcgt cggtcggcgc taaaaccaga gagtttatcg gcgtgctgga gacgctcggc
      961 ttggtcgata aaaagaaaac gaccaaaacg ctgattgtcg attcgctcga caacgccaat
     1021 ctgattcgct cgtcgcgcaa cgtgcagaaa accaaagtca ccaacagtta cggcttaaat
     1081 atttatgatt tgctgtatca cgagcggctt ctggtttcca aaacggcaat cgaagagttg
     1141 agccaaatgc tcgacccgac gcgcgaaaga ggcagcgcca attcaaacgg agcgacagcg
     1201 gtagaaacgt ccgcgacgga cagggcgggc gaaaatacag aggaggcggc gaatgagtaa
     1261 agttctgaat atgaatgtct gggacattct aaagacgccc gttatcacgg aaaaatcggt
     1321 tcttttgaaa gagcagagca gcgaagaagg cgaagcgcag gttttgagct tcaaagttga
     1381 ccgcaaagcg tcgaaaaacg acattcgcaa agcggtcgag gaaatcttca gcgtgaaggt
     1441 ttccaagatt cgcaccgtca attacgaagg caaaattaag aaacgcggaa gatacgaagg
     1501 tcgccgcgca tcgtggaaaa aggcttatgt gaccttgcag aaaggcgagc cgattgtgga
     1561 ttacgcggaa gcaatttaat tagctgataa ctgatagtgg ataactgata gttgaaacca
     1621 attattatca gttatcagat atccactatc cactaaagaa atgggcatta agaaattaaa
     1681 accgacatca ccggcgcgtc gctatcaaac atatctgacg cgcgatgaac tgactccgaa
     1741 cgcaaagccc gaaaaatctt tgctggagcc gaaaacaaga atttccggtc gcaacaatga
     1801 cggtcgcatc acggttcgcc gtcgcggcgg cggtcacaag cgtttctatc gcatcgtcga
     1861 tttcaagcgc aacaaaaccg gcattcccgg tcgcgtgacg caaatcgagt acgacccgaa
     1921 tcgttcggcg catatcgcct tgattactta tctggacggc gaaaaacgct acattctcgc
     1981 gccggtcggc ataaccgtcg gttcgaccgt gatgagcggc gcggaagcgg acattctgcc
     2041 gggcaacgct ctgccgattc aaaacattcc gctcggaacg cagattcaca atatcgaact
     2101 gcgtcccggc aaaggcgggc agatggcgcg ctcagccgga agtttcgcgc agctggtcgc
     2161 caaagagggc gatttcgcgc agctgagaat gccttcgggc gaggttcgcc gcgtgcccgt
     2221 ggtctgtatg gcgaccgtcg ggcaggtcgg caatacggaa aacgaaaacg tcagcctcgg
     2281 taaagccgga cgctcgcgct ggaaaggctt gcgaccgaaa gttcgcggcg tcgcgatgaa
     2341 tccggtcgac cacccgcacg gcggcggcga aggcaaaact tcgggcggac gccatccggt
     2401 aacgccctgg ggacagccga cgcgcggtta taaaactcgt aataacaaac gcacgcagaa
     2461 atttatcgtg cgcgacagaa gaagcaagta gtaaaaggat gaattatgaa cgcggaacgc
     2521 tgaagaaaga aattcatcat tcatcgttcc cggttcatca ttaaaaattt atgccacgtt
     2581 cagttaaaaa aggacctttt attgatttaa gcgtccagcg aattttgaga aaaatcagcg
     2641 aaagcggctc gaaaccgcaa gcgattaaaa catggtcgcg ccgctcgaca attacgccgg
     2701 aaatggtcgg tttgaccttc ggcgtgcaca acggcaaacg tcacgttccg gtttatgtta
     2761 cggaaaatat ggtcgggcat aagctcggag aattttcgcc gaccagaact tttaaaggac
     2821 acccgggaac aaaagccgag aagatggcga agagaaaata gtgaaaaacg aaaagtgaaa
     2881 aattaaaaaa agatttcact attcactatt cactattcac taaaaagatt atggaagcga
     2941 tagcaaaaac aaaacatttg aaaggttcgc cgcgcaaggc gcggctcgtg attgatatga
     3001 ttcgcggcgt gaatgtttcg agagccctgg cgattctgaa attcacggac aaacgggcgg
     3061 ctgacccgat tgccaaaacg ctgctgtcgg cgattgccaa cgcgacgttt aaggcggaaa
     3121 aacagaacat cgccatcgac ccggacgaat tgttcgtcaa atcgtgcttt gtcgatatgg
     3181 gagcgcacaa aaatcgccgt cgtatgcgtc ccgcgccgca gggtcgcgct tatcgcgaac
     3241 agcgtcacta ttgtcatatt acgattgagg tttcgagcga caagcccgaa gaaggcaaag
     3301 tcagaaacgc gaaaatcacc ggcgaagagg aaacgaagaa atccacttcg aagcgggcgg
     3361 cgattaagaa aaccgacgcg ggcgaaagaa ccgcaaaagc ttccgcccaa aaagccgacg
     3421 aggtgacgga aagccgagca gcggcgcacg aagctaacgt cgaagaagta gaaacgacgg
     3481 cgacaacagc gacgacggcg accgaacaaa ccgccggaga aacaacgacg gcggcaaccg
     3541 aagaaacttc gccgaaagcg acggaaagct cgaaagcggt taccgaagaa gcaaccgaag
     3601 gcgatgccgc cgcgtcggac gcgaaagata cggcgacgca gtttcccgca gaattaaacg
     3661 aaaccaaacg ggctgacga
//
LOCUS       DASWNE010000265         1749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_291187, whole genome shotgun sequence.
ACCESSION   DASWNE010000265 DASWNE010000000
VERSION     DASWNE010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291187"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VGB68_05500"
     CDS             complement(<1..387)
                     /locus_tag="VGB68_05500"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0048718.1"
                     /translation="MSEKLGLVQRVEFIKQACRGKKVLHLGCTDYPFTEKMIEFNQLL
                     HFELAKIAGELYGFDFDQKGIDILAASGTKNLYRADLEKLEEVDLNEKFDVIIAGEMI
                     EHLSNPGLFLRGIKRFMHRETILLITT"
     gene            complement(416..>1749)
                     /locus_tag="VGB68_05505"
     CDS             complement(416..>1749)
                     /locus_tag="VGB68_05505"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEY0048719.1"
                     /translation="SFVFSIGRAITKYVAEYQATGESGKIKDIIAATFFINLVLGLVG
                     IISVFSLSGWLVSDVMNIAPESHSRTVIALYIASTAIFFTLFTQVFSAIVQGLHRFDI
                     YSRILNFSSISMLLGNLIIAWLGGGLVALFWWNLFITCATCALFYASAKKLLPDFSVT
                     RRFDKESLKLVIGFSAGVVGYQVLGNIWLLFERIWITRHLGTESLTHYVVPMTVSFQI
                     QYFIASLVIVVFPLASELQNDLEKLERLYTKASKIITMMVVFIGATIIVHSHLFLTLW
                     MGAEFADASAHLLVIHTVSFSLLAILTVSWQMTEGLGYPNFYCYLYVLCLLISIPSMV
                     FFSYDYGNTGVAIGRLVGYAISFLSILYVEKWFFKRILAAFWLNVLGRLLFAAAATVS
                     VEILSIDYLSAGWLALFLSSFLGGVTYLSVLWLLGFVTEDEKILVRNVFGT"
BASE COUNT          535 a          436 c          372 g          406 t
ORIGIN      
        1 cgtcgtgatg agcagaatcg tttcccggtg cataaagcgc ttgatgccgc gcaggaaaag
       61 tccgggattt gataaatgct cgatcatctc gcccgcgatt atcacgtcga atttctcatt
      121 taaatcgact tcttcgagct tttccaaatc cgcacgataa agattttttg ttccgctcgc
      181 ggctaaaatg tcgattccct tctggtcgaa atcgaaaccg taaagctcgc ccgcaatttt
      241 cgccagttca aaatgaagaa gctgattgaa ttcaatcatc ttttcggtaa atggataatc
      301 cgtgcagccg agatgcaaaa ctttttttcc gcgacacgct tgcttgataa attcgaccct
      361 ttgcacaagt cctaattttt cgctcatcta acctttatat ttatgataaa tttatttatg
      421 tcccgaaaac gtttctgacc aaaatctttt cgtcttcggt gacaaatccc agcaaccaca
      481 aaaccgacag atacgtaacg ccgccgagaa agctcgataa aaacagcgcc agccagcccg
      541 ccgataaata atcgatgctc aaaatctcga ccgaaacggt cgctgccgcg gcaaaaagca
      601 gacgccccag aacatttagc cagaaagcgg cgagaatccg tttaaaaaac catttttcga
      661 cgtatagaat cgaaagaaat gaaatcgcgt acccgaccag gcgaccgatt gccacacccg
      721 tatttccgta atcgtaacta aaaaaaacca tcgacggaat actaattaat aaacatagca
      781 catagagata acaataaaaa ttcgggtagc cgagaccttc ggtcatctgc cacgaaaccg
      841 tcaaaatcgc cagcaaacta aagctgaccg tgtgtataac cagcaggtgc gcgctcgcat
      901 cggcaaactc cgcgcccatc cacagagtca aaaacagatg gctgtgtacg attatcgtcg
      961 ctccgataaa aacaaccatc atcgtgatta ttttcgacgc tttggtataa agcctttcga
     1021 gtttttcgag gtcgttttgc aattcgctgg cgagcgggaa aacgactatt acgaggctgg
     1081 cgataaaata ttgaatctga aagctgaccg tcatcggaac cacataatgc gtcaggcttt
     1141 ccgttccgag atggcgcgta atccatatcc gctcgaaaag cagccagatg ttacccagca
     1201 cctgatagcc gacgacgccc gcgctaaaac cgatgacaag ttttaacgat tctttatcaa
     1261 aacgacgagt tacggaaaaa tccggcagaa gttttttggc gctcgcataa aacaatgcgc
     1321 aggtcgcgca ggttataaac aaattccacc agaataaagc gacgagcccg ccgccgagcc
     1381 aggcaatgat taaattgccg agcagcatcg aaatgctgga aaagttcagt attctggaat
     1441 atatgtcaaa gcggtgaagc ccctgcacaa ttgcgctgaa aacctgcgtg aacagagtga
     1501 agaaaattgc cgtagatgca atgtacagag caattacggt tctggaatgc gattccggcg
     1561 cgatattcat aacgtcggaa accagccagc cggaaagcga aaaaaccgaa attattccca
     1621 ctaaaccgag caccagattg ataaaaaaag ttgcggcaat aatgtctttt atctttccgc
     1681 tttcgccggt cgcctgatat tcggcgacgt atttcgtgat ggcgcgcccg atgctgaaga
     1741 caaacgaat
//
LOCUS       DASWNE010000266         4658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_573645, whole genome shotgun sequence.
ACCESSION   DASWNE010000266 DASWNE010000000
VERSION     DASWNE010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4658
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573645"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VGB68_05510"
     CDS             <1..338
                     /locus_tag="VGB68_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048720.1"
                     /translation="QVQKMENKAEYCLIVEGNYFDESEAEHALRDPFIEEFVEQSDNF
                     RLHNFDDIEVACGISLGELMVSMIDDEMFKVSCQNRTRPLTEHTAKLLAEALRRQDMF
                     DEIRVEPLH"
     gene            416..964
                     /locus_tag="VGB68_05515"
     CDS             416..964
                     /locus_tag="VGB68_05515"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: protein motif:HMM:NF001664.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEY0048721.1"
                     /translation="MLTEFFSGRVAVKVGDITCEQVDAVVNAANSALLGGGGVDGAIH
                     RAGGARILEECKEIRRTKFPEGLPTGKAVITSGGNLPARFVIHTVGPVYGMNQGADAA
                     LLADCYRNSLLTAVENNLETIAFPAISTGVFAYPRDEAARVSSEAIKNFLEDNQTIKQ
                     VRLVFFSAPDAEKFVKHQVFDE"
     gene            1153..3324
                     /gene="glgP"
                     /locus_tag="VGB68_05520"
     CDS             1153..3324
                     /gene="glgP"
                     /locus_tag="VGB68_05520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEY0048722.1"
                     /translation="MTQAAGQQLEHYFRRDFSINRQLPENLQILDELSKNFYWSWTAE
                     GAEIFRDLDAPLWEKVEQNPRRMLKEIGELRLWQKSCEPSYVERVRAFSGRSDEYLAA
                     PTNSFGRITRKSPVAYFCAEYGVHNSLPIYSGGLGILAGDHLKSASDMNVPLVAVGLF
                     YRFGYFRQKLAHTGWQEERYLDAFENQLPLEPVLDENGERISVTVQMRGREVAAQAWL
                     ARVGRISLYLLDTNVLQNAEVDRLITGHLYGGDTETRVVQEEILGIGGVRLLRKLGIE
                     PAVYHLNEGHSAFLTLELAREFLQENAAQSFTDAVSGVREKCVFTTHTPVSAGNDTFA
                     PALLEKCFDENFLAALKLSKEEFFAFGRTDTGDAHEWFGMTPFAIRMCRSANGVSEKH
                     GEVSRALWQSMFGNAQIEEVPITFVTNGVHAPTWIAPALKAVYEKHIGADWTEILKDE
                     KAWRGAIERIPDEEIWRAHNLLKNQLIAFIRQRTFAADTGSMETIHEHSDVKNFFEPH
                     VLTIGFARRVAAYKRWNLLLSDVPRLQRMIGDAKRPVQFVFAGKAHPQDRTAKALLQN
                     LMNQKQNGTWLQRAAFIEDYDQEVARYLVQGVDVWLNVPRRPLEASGTSGQKAAMNGG
                     LNLSILDGWWIEGAGGENGFSLGVLRDEGDLSDDETDANDAESLYRVLETEVIPAFYE
                     KNADGLPHVWIAKMRSALATLTPQFSSDRMLKDYIEKIYGA"
     gene            3573..4298
                     /locus_tag="VGB68_05525"
     CDS             3573..4298
                     /locus_tag="VGB68_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048723.1"
                     /translation="MQNTEFRTGVIRPVECFKEGWELIKDQYWLFFVIVLVGMLIGSF
                     IPLGIGLGAMFCGIYYCLFQKMNGQKVEFEGLFKGFNYFVPGLVATLVLIVPAVISAL
                     FVYASMIAVLFGSMDSRGNLNETAIWAMMGTVFIEIIALWLILGSLHVFLMFSYPLIV
                     EKNMSGWAAFKLSARAGWANLSGVTGLILIEFVLGLAAYVLTIFVLGFGVYLVMPLMF
                     AGVLVAYRKVFPSAESFTPPNAF"
     gene            4323..4655
                     /gene="ytxJ"
                     /locus_tag="VGB68_05530"
     CDS             4323..4655
                     /gene="ytxJ"
                     /locus_tag="VGB68_05530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HEY0048724.1"
                     /translation="MKANFTKLDSSERLEELFQKSHEAPVLVFKHSTTCPISAAAYRQ
                     VEDVDGDVNIIIVQTARGISNEIANRTGIRHESPQAIVLKDGKPVYHASHYSVTAEEV
                     NKTMNAER"
BASE COUNT         1113 a         1128 c         1302 g         1115 t
ORIGIN      
        1 cgcaagttca gaaaatggag aataaagcag aatactgttt gattgtagaa ggcaattatt
       61 ttgacgagtc ggaagccgaa cacgccttgc gtgacccgtt tatcgaggag tttgtcgaac
      121 agagcgataa ttttcgactg cataattttg acgacatcga agtcgcctgc ggcatcagtc
      181 tgggcgaatt aatggtttcg atgatcgacg acgagatgtt caaggtctcg tgtcagaacc
      241 gcacgcgccc gctgaccgag cacacggcga aactgctggc ggaggcgctc cgccgccagg
      301 atatgtttga tgaaatccgc gtcgagccgc tgcattaaaa caaaataaaa actactgcgc
      361 aatagacagg ataagaaaaa ttttctattc tcgtaatcct gtcttttcta tttttatgtt
      421 aacggagttt ttcagcggtc gcgtcgcggt caaagtcggc gatattacgt gcgagcaggt
      481 cgatgcggtc gtcaacgcgg caaattccgc tttgctcggc ggcggcggcg ttgacggcgc
      541 gattcaccgt gcgggcggcg cgcggatttt ggaagaatgt aaggaaattc gccggacgaa
      601 gtttccggaa ggcttgccga cgggaaaagc cgttatcact tccggcggaa atttgcccgc
      661 gcgtttcgtc attcacacgg tcggtcccgt ttacgggatg aatcagggcg cagacgccgc
      721 gcttttagcc gattgctacc gcaattcgct gctgacggcg gttgaaaaca atctggaaac
      781 gattgccttt ccggcgattt cgaccggcgt ctttgcttac ccgcgcgacg aagcggcgcg
      841 agtttcttcc gaagcgataa agaatttttt agaagataat caaacaatca aacaggtgcg
      901 gctcgttttt ttttcagcgc ccgacgccga aaaatttgta aaacatcaag tgtttgacga
      961 ataattgtag aagaaaaaga atcgaagccg ccgacagttc gggattaatt aggataaaat
     1021 tccgtttaac gcgtgattta tcttaatcaa tgggcgtcct cttttcgcct ttgtttagag
     1081 ggtgaacaaa gattgccgcg gcgggctttt taacgcagat aattatttat gaaggagtct
     1141 tccatcgaag aaatgacaca ggcagcaggt cagcaattag agcattattt ccgtcgggat
     1201 ttttcaatca accgtcagct tccggaaaat ttacaaattt tagacgagct ttccaaaaat
     1261 ttttactggt cgtggacggc ggaaggcgcg gaaattttcc gcgatttgga cgcgccgctc
     1321 tgggaaaaag tcgagcagaa tccgcgccgg atgctgaaag aaatcggcga attacggctc
     1381 tggcagaaat cgtgcgagcc gtcttacgtc gaaagagttc gggctttttc ggggcgctcg
     1441 gacgaatatc tcgccgcgcc gacaaattct ttcggcagaa tcaccagaaa aagtccggtc
     1501 gcttattttt gcgccgaata cggcgtccat aattccctgc cgatttattc gggcgggctt
     1561 ggaattctgg cgggcgacca tctgaaatcg gcgagcgata tgaacgttcc gctggtcgcc
     1621 gtcgggcttt tttatcgttt cggctatttt cgtcaaaagc tggcgcacac gggctggcag
     1681 gaagaaagat acctggacgc gtttgaaaac cagcttccgc tggagcctgt tctggacgaa
     1741 aacggcgagc ggatttcggt caccgtgcag atgcgcgggc gcgaagtagc ggcgcaggcg
     1801 tggctggctc gcgtcggcag aatctcttta tatctgctcg acacaaacgt tttgcaaaac
     1861 gccgaagtcg atcgccttat caccggacat ctttacggcg gcgacacgga aacgcgcgtc
     1921 gtgcaggaag aaattctcgg catcggcggc gtgcggcttt tgcgaaaact cggtatcgag
     1981 cctgcggttt atcatctgaa cgaaggtcat tcggcgtttc tgacgctgga gctcgcccgc
     2041 gaatttttgc aggaaaacgc cgctcaaagc tttaccgacg cggtttccgg cgtgcgcgaa
     2101 aaatgcgttt tcacgacgca tacgcccgtc tccgccggaa acgacacttt cgcgcccgca
     2161 ctgctggaaa agtgtttcga cgaaaacttt ctcgccgcgc ttaagctttc aaaggaagaa
     2221 tttttcgcct tcggtcgcac cgacacgggc gacgcgcacg aatggttcgg aatgacgccg
     2281 ttcgccattc gtatgtgccg ctcggcaaac ggcgtgagcg aaaaacacgg cgaggtttcg
     2341 cgcgcgctgt ggcagagtat gttcggcaac gcgcagattg aagaagttcc gataactttt
     2401 gtgaccaacg gcgttcacgc gccgacctgg attgcgccgg ctttaaaagc cgtctacgaa
     2461 aaacacatcg gcgcggattg gacggagatt ttaaaagacg aaaaggcgtg gcgcggggca
     2521 atcgaacgga ttcccgacga agaaatctgg cgcgcgcaca accttttaaa aaatcagctt
     2581 atcgccttta tcagacaaag aactttcgcc gccgacacgg gctcgatgga aactattcac
     2641 gagcattcgg acgtgaaaaa cttttttgag ccgcacgtgt tgactatcgg ctttgcgcgc
     2701 cgtgtggcgg cgtataaacg ctggaatctg cttttgtcgg acgtgccgcg attgcagaga
     2761 atgattggcg atgcgaagcg tccggtgcaa ttcgtttttg ccggaaaagc gcacccgcaa
     2821 gaccgcaccg cgaaagctct tttgcaaaat ctgatgaacc agaaacagaa cggaacgtgg
     2881 ctgcagcgcg ccgcttttat cgaggattac gatcaggaag tggcgcgtta cctggtgcag
     2941 ggcgtcgatg tctggctgaa cgttccgcgc cgcccgctgg aagcttccgg cacgagcggg
     3001 cagaaggcgg cgatgaacgg cggcttgaat ctttcgattc tggacggctg gtggattgaa
     3061 ggcgcgggcg gcgaaaacgg tttttccctc ggcgtactgc gcgacgaagg cgatttgagc
     3121 gacgacgaaa cggacgccaa cgacgccgaa tcgctttatc gagttctgga aaccgaggtg
     3181 attcccgcat tttacgaaaa aaatgccgac gggctgccgc acgtctggat tgcaaaaatg
     3241 cgcagcgctc tggcgacgct gacgccgcag ttttccagcg atagaatgct gaaagattac
     3301 atcgaaaaaa tttacggcgc gtgaagaata aaccgccggg agcgcttgcg gcgcacgaga
     3361 aggaattaac gaagaggata aaccggggat aataaaaaca tttataaaat ccctgtccat
     3421 ccggcttcat ccccggttaa ttctctttct gaaatcacag tgccgccgcg gctttcggcg
     3481 gcggaaattg ttgttattcc gaaaaggaat gtggataatg aaagcgggaa attttccgtt
     3541 cgcttcatta tccatttttc gtagaggtca aaatgcaaaa tacggagttc aggacgggcg
     3601 tgattcgccc cgtcgaatgt ttcaaagaag gctgggaatt aatcaaagac caatactggc
     3661 tgtttttcgt catcgtgctg gtcgggatgc tgattggcag cttcattccg ctcggcatcg
     3721 gactgggcgc gatgttttgc ggcatttatt attgcctgtt tcaaaaaatg aacgggcaga
     3781 aagtcgagtt tgaaggactt ttcaaaggct ttaattattt tgttcccggg ctggtggcga
     3841 cgctcgtgct gatcgttccc gccgtgattt cggcattatt cgtttacgcc tcgatgattg
     3901 ccgtgctttt cggctcgatg gattcgcgcg gcaaccttaa cgagacggcg atttgggcaa
     3961 tgatgggaac ggtttttatc gaaataatcg ctctctggct cattctgggc tcgctgcacg
     4021 tttttctgat gttctcgtat ccgctgattg tcgaaaaaaa tatgagcggc tgggcggcgt
     4081 tcaaactcag cgcgcgcgcc ggctgggcaa atctttcggg cgtcaccgga ttgattctga
     4141 tagagtttgt gcttgggctg gcggcttacg ttctgactat tttcgtgctc ggattcggcg
     4201 tttatctggt gatgccttta atgtttgcgg gcgttctggt cgcttaccgg aaagtttttc
     4261 cttcggctga aagctttacg ccgccgaatg cattttgata atcgaaaagt tataatcggg
     4321 ttatgaaagc aaattttacg aaactcgatt ccagcgagcg gctcgaagag ctttttcaaa
     4381 aatcgcacga agctccggtt ctggttttca aacactcgac aacctgcccg attagcgccg
     4441 ccgcttatcg tcaagtcgaa gacgtggacg gcgacgtgaa tataattatc gtgcaaacgg
     4501 cgcgcggcat ctccaacgag attgcgaacc gcaccggaat ccgccacgaa tcgccgcagg
     4561 cgatcgttct gaaagacggc aagcccgttt accacgcgtc gcattacagc gtgacggcgg
     4621 aagaagtcaa taaaacgatg aacgcggaac gatgaacg
//
LOCUS       DASWNE010000267         3751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_573667, whole genome shotgun sequence.
ACCESSION   DASWNE010000267 DASWNE010000000
VERSION     DASWNE010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573667"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="VGB68_05535"
     CDS             <1..1098
                     /locus_tag="VGB68_05535"
                     /inference="COORDINATES: protein motif:HMM:NF012427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0048725.1"
                     /translation="PDLNPEDDRELAREIMDAKTGTEKLLKEILLPNVRPMYDDLTRA
                     VEGADLFVTGEVVFAAKSVHEKTGIKWVTTSLAPASFLSAHDPFVPPTAQWLKHFRFL
                     GSGFYGLLFGLIRRLVKSWLGDYRKFRRELGLDENHDPVFEEKFSEQLHLALFSKVLG
                     KPQPDWHSPTLQCGFCFYDGQNDLGKMPEGLQKFLDAGEPPIVFTLGSAAVMDARDFF
                     EQSAEAARILQRRAVLLYGIFNEPPKTTLDDSVVAFDYAPYSMVFPRVAGVVHQGGVG
                     TTAQVLRAGVPHLFMPYSHDQPDNAARCERAGVARVISRDGYKAQNAAGELRKLLSDS
                     SYKTNALEAKRIVDAEHGTGMACDAIEEMLK"
     gene            1299..1958
                     /gene="can"
                     /locus_tag="VGB68_05540"
     CDS             1299..1958
                     /gene="can"
                     /locus_tag="VGB68_05540"
                     /inference="COORDINATES: protein motif:HMM:NF007756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HEY0048726.1"
                     /translation="MSKLKHLLDDNKKWARQITEDNPDFFENLARLQNPQYLWIGCSD
                     SRITPAVSIGLLPGELFVHRNIANLVVHTDMNCLSVMQFAVEALKVKHIIVCGHYNCG
                     GVLAAFENARLGLIDNWLRHIQDIAKDFKDLLDAATEDKKLDLLCELNVVEQVMNVGE
                     TTILQDAWMKGQEVAIHGWIYRLDEGIFQDLNVTIESLADLTELREKSLTKRKKFGVG
                     K"
     gene            1955..>3751
                     /locus_tag="VGB68_05545"
     CDS             1955..>3751
                     /locus_tag="VGB68_05545"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEY0048727.1"
                     /translation="MRKLIFILLLLSLFAPDAFGQMSRKPSPTRAPAGAMSKKPDSIK
                     RALNAIRTQAEFDSIARTYHQGTPFALPHAMFVIDRKNKNKIYYVNSQRFRFHKDFLL
                     ANYLVARGEDVFKPIYIDENRRFIVGTIAWQKPVEKFTWELWEGDLATAEHIKLAGDV
                     INKTFFQEVAYKPNSIRQEDVSAELGIQRVLQSDIAKNQEYLALNTGKAIGRIHVIEK
                     LDDTVEIGDNEILVLKELPLNLPPVRGIIVAKPSTPLSHVNILAKDWNIPNVYIKDAD
                     KLFKEFDTYWVNLEADLTDYKIKRIADYEPGKAPPLPRSPDAQIPPVNLNVKKLAGLR
                     EMRKKDSVIYGSKAANLGEMLNAKAAGFIVPDGFSVPFYWYDKFIQDNGIDKIILELI
                     DNNDFVHNPRIRRQKLEELRAQIQNGRFDEDLKQQVVEKWRAQLGGKSVFVRSSSNSE
                     DLPNFSGAGLYSSVANVKEEEKLVEAVKKVWASLWKFEAYEARVRNYVSQTDVYMSAL
                     IQHSVDMDRGGVMITKDPFDEENENAVYISVVCGHNSKVVDNKGIPEQILFNPKSNSV
                     VLMTFSQQENAFIFDESGGLKETADKCADKSSK"
BASE COUNT          957 a          898 c         1016 g          880 t
ORIGIN      
        1 cctgacctga atcccgagga tgaccgagaa ctcgcgcgcg aaataatgga cgcgaaaacg
       61 ggaacggaaa aattacttaa agaaattctt ttgccgaacg tgcgcccgat gtatgacgat
      121 ttgacgcgcg cggtcgaagg cgcggattta ttcgtcacgg gcgaagtcgt tttcgccgcc
      181 aaatccgtcc acgaaaaaac cggaatcaaa tgggttacga caagcctcgc gccggcttcg
      241 tttctttccg cgcacgaccc gtttgtgccg ccgaccgcgc aatggctgaa acattttcgt
      301 tttctcggaa gcggttttta cggtttgctt ttcgggctga tacgccgcct ggtgaaatcg
      361 tggctcggcg attaccggaa gtttcggcgc gagctcggct tggacgaaaa tcacgacccg
      421 gtatttgaag aaaaattttc ggagcagctt catctggcgt tgttttcgaa agttttggga
      481 aagccgcagc ccgactggca ttcgccgacc ttgcagtgcg gtttttgttt ttacgacggg
      541 cagaacgatt tgggaaaaat gcccgaaggc ttacagaaat ttctcgatgc gggcgagccg
      601 ccgattgttt ttacgctcgg ctcggcggcg gtcatggacg cgcgcgattt ttttgagcag
      661 agcgcggaag cggcgcggat tttgcagcgc cgcgccgttt tgctttacgg cattttcaac
      721 gagccgccga aaacgactct ggacgatagc gtagtcgcct ttgattacgc gccgtattcg
      781 atggtttttc cgcgcgtggc tggcgtcgtc catcagggcg gagtcggcac gacggcgcag
      841 gttctgcgcg cgggcgttcc gcatcttttt atgccttatt cgcacgacca gcccgacaat
      901 gcggcgcgct gcgagcgggc gggcgtcgcg cgcgtgattt cgcgcgacgg gtacaaggcg
      961 caaaatgcgg cgggcgaatt gcgaaaactt ctttccgatt cgagttataa aacaaacgcg
     1021 ctcgaagcga aacgcatcgt agatgccgag cacggaaccg gaatggcgtg tgacgcgatt
     1081 gaggaaatgc taaaataata agagtttgca ttaagaaaat gaaaccacgg atttacacgg
     1141 ataaacgcgg atttttaacg ggatttacaa gataagcagg ataaaatcta tttcttatcc
     1201 tgcttatctt gtaaatccgc ggtttcattt ttctttgtat tcttgatgat gaaaaatcct
     1261 tatttcaaac acgtgttaaa atatttcttt taaaaattgt gagtaaatta aaacatcttt
     1321 tagacgacaa taaaaaatgg gcgcggcaga taacggagga caatcccgat ttttttgaaa
     1381 atctcgcgcg gctgcaaaac ccgcaatatc tgtggattgg ctgttcggac agccgcatca
     1441 cgccggcggt cagcatcgga cttctgccgg gcgagctttt cgtgcatcgc aacatcgcca
     1501 atctggtcgt tcataccgat atgaattgtc tttcggtgat gcagtttgcc gtcgaggcgt
     1561 tgaaagtaaa acatataatc gtctgcggtc attacaactg cggcggcgtg ttggcggcgt
     1621 ttgaaaacgc ccgactgggt ttgattgaca actggctgcg gcacattcag gacatcgcca
     1681 aggatttcaa ggatttgctg gacgcggcga ccgaggataa aaaactcgat ttgctgtgcg
     1741 agctgaacgt cgtcgagcag gtgatgaatg tcggcgaaac gaccattttg caggacgcct
     1801 ggatgaaagg gcaggaagtg gcgattcacg gctggattta ccggctcgac gagggaattt
     1861 ttcaggattt gaacgtgacg attgagagcc ttgcggattt gactgaatta agagaaaaat
     1921 ctttgacgaa acgtaaaaaa tttggagtcg gaaaatgaga aaactgattt ttatcctgct
     1981 tttgctttcg cttttcgcgc cggacgcttt cgggcagatg tcgcgcaagc cttcgccgac
     2041 gcgcgcgcct gccggcgcga tgtcgaaaaa gccggattcc ataaaacgcg ctttgaacgc
     2101 aatcaggact caggcggaat ttgattcgat tgcgcgcacc tatcatcagg gaacgccctt
     2161 tgccctgccg cacgcgatgt ttgtcatcga ccgtaaaaat aaaaacaaaa tttattacgt
     2221 caactcgcag cggtttcgtt ttcacaaaga ttttcttctg gcaaattatc tggtcgcgcg
     2281 cggcgaagac gttttcaagc cgatttacat cgacgaaaac cgtcgtttta tcgtcggcac
     2341 gatcgcctgg caaaagccgg tcgaaaaatt cacctgggaa ttgtgggaag gcgatttggc
     2401 gaccgccgag cacatcaagc tggcgggcga cgtcatcaac aaaactttct ttcaggaagt
     2461 cgcctacaaa ccgaactcga ttcggcagga agacgtctcg gctgaactcg gcatccagcg
     2521 cgttttgcaa agcgacatcg ccaaaaatca ggaatatctg gctctgaaca cgggcaaggc
     2581 aatcgggcgc attcacgtta tcgaaaagct ggacgacacg gtcgaaatcg gcgataacga
     2641 aattctggtt ttaaaggaac tgcccctgaa tcttccgccc gtgcgcggca ttatcgtcgc
     2701 caagccttcg acgccgcttt cgcacgtcaa cattctggcg aaagactgga atattccgaa
     2761 cgtttatatc aaggacgccg acaagctttt caaggaattc gacacatatt gggtcaatct
     2821 ggaagccgat ttaaccgatt ataaaataaa acgaatagcc gactacgagc ccggcaaagc
     2881 gccgccgctt ccgagatcgc cggacgccca aattccgccc gttaatctaa acgtcaaaaa
     2941 gctcgccggt ctgcgcgaga tgcgcaaaaa agacagcgtc atttacggct cgaaagcggc
     3001 gaatctcggc gagatgctga acgcgaaagc ggcgggcttt attgttcccg acggctttag
     3061 cgtgcctttt tactggtatg acaagtttat acaggacaac ggcatcgaca aaattattct
     3121 ggaattgatt gacaataatg attttgttca caacccgcgc attcgccgtc agaaactgga
     3181 agagctgcgc gcgcagattc agaacgggag attcgacgag gatttaaaac agcaggtcgt
     3241 cgaaaaatgg cgcgcgcagc tcggcggaaa atccgttttc gtgcgcagct cttccaactc
     3301 ggaagatttg ccgaattttt ccggcgcggg actttattcg agcgtggcga acgtcaagga
     3361 agaagaaaag ctcgtcgaag cggtgaaaaa agtctgggct tcgctctgga aattcgaggc
     3421 ttacgaggcg cgcgtgcgca attacgtcag ccagacggac gtttatatgt cggcgctgat
     3481 acaacacagc gtggatatgg acaggggcgg cgtgatgatt accaaagacc cgtttgacga
     3541 ggaaaacgaa aacgcggttt atatcagcgt ggtctgcggg cataattcga aagtagtcga
     3601 taacaaaggc attcccgaac agattttgtt taatccgaaa tcgaattcgg tcgtcctgat
     3661 gaccttttcg cagcaggaaa acgccttcat ttttgacgaa agcggcggtt taaaggaaac
     3721 cgccgacaag tgcgcggata aatcgtcgaa a
//
LOCUS       DASWNE010000268         3103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_43683, whole genome shotgun sequence.
ACCESSION   DASWNE010000268 DASWNE010000000
VERSION     DASWNE010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43683"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VGB68_05550"
     CDS             <1..290
                     /locus_tag="VGB68_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048728.1"
                     /translation="FVFEFKYEPLSEAAIPLKLPRYVYDNVLRLEPIENYATPMFVIL
                     FLSLQAINFFIYSIPFYLVFTLNNLYTGKKSSGKTEELKVPPSPPGFEDSK"
     gene            complement(367..2493)
                     /locus_tag="VGB68_05555"
     CDS             complement(367..2493)
                     /locus_tag="VGB68_05555"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY0048729.1"
                     /translation="MKIELEIPQSLSLERKIKMHEAREGIIDRLARDLPTNIDLERFL
                     NVVVSEIGRMMDADRCDLLQLTEGNELRISHEWRKDETVPPSEGTMIPFDAQKLTEKF
                     DLSQPVRINDTTKTKDATVRFFTKALETQSLLVVPITLNEKVIGLLGLHDTHAPREWL
                     DEEVAFLESVGRQLAIGYQYTRLYVAQEQETRRTNALLEIANTLNSHSDFNEVSARVL
                     ERAVELVGANYGALGVLDQTGKKISLASFKSAPNVEPNAVLQMIEEHNQSLDIEPFPV
                     LSDILREGKTLRLVDKDLPFAVRLIFNSQLDGRAALVAPVRVGGQAFGLLGFVWSEER
                     DGGFENHEVALVEGISDQIGTALERDQLSAEVMRLKSALHQKHQSEIIGQAPTIRRAI
                     ELALNVADTQTTVLIQGESGTGKELLANLIHYNSGRENKPYVKLNCGAIPETLLESEL
                     FGHEKGAFTDARNQRKGRFEEADRGTLFLDEIGEMPLAAQVKLLRVLQDGEFTRVGGN
                     ETVKTDVRVIAASNVNLEKAVEEGKFRKDLFYRLSVFPITLPPLRERPEDIHILVFHF
                     LEQYKQKTGRFVSGISKEALRALVNYEWSGNVRELENAIERAVIIASGRQIELDDLPE
                     AISKKAFESFAQARQERAKAASEGRAINLEVEFPATMEEIEKQAIELALDYTSGDKSR
                     ASRLLNIGRKTLYRKLEQYENGKTSD"
     gene            2829..>3103
                     /locus_tag="VGB68_05560"
     CDS             2829..>3103
                     /locus_tag="VGB68_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048730.1"
                     /translation="MKRILTRMAALALLVIATFWFGADTQAKKWGCADSMFAAQSACD
                     DTLSGTIGSYNNVTNFSPNQCDNQAQVQCGNNSTCYNSTYNNCYNSAV"
BASE COUNT          727 a          809 c          747 g          820 t
ORIGIN      
        1 attttgtatt tgaatttaag tatgaacccc tgtcagaagc cgcaattccg ctcaaacttc
       61 ccagatatgt ctacgacaat gttttacggt tagagcctat tgaaaactat gcgacaccaa
      121 tgtttgtaat actttttttg agtctacagg caattaattt cttcatatat tccattcctt
      181 tttatttagt ctttacttta aacaatttat acactggaaa gaaatcaagc gggaaaacgg
      241 aagaactgaa agttcctccg tcaccgcccg gttttgaaga ttcaaaatga aatccgctaa
      301 agtttgaaaa aaaattgatt aatcattttt tttcgagact gcgttctttc acctgcttcg
      361 cggtttttaa tccgaggttt ttccgttctc gtattgttcc agctttcgat aaagcgtttt
      421 tctgccgata tttaataatc tcgacgcgcg cgatttgtcg ccgctcgtgt aatcgagcgc
      481 cagctcgatt gcctgttttt ctatttcttc catcgtcgcc ggaaactcga cttcgagatt
      541 tatcgcgcga ccttcgctcg ccgctttggc gcgttcctgc cgcgcctgcg caaatgattc
      601 gaacgctttt ttgctgatgg cttccggcaa atcgtcaagc tcgatttgcc gaccggaagc
      661 gataatcacg gcgcgctcaa tcgcgttttc cagctcgcgg acgttgcccg accattcgta
      721 attaaccagc gcgcgcaggg cttcttttga gattcccgac acgaagcgac cggttttttg
      781 cttgtattgc tcgagaaaat gaaaaaccag gatgtgtatg tcttccgggc gctcgcgcag
      841 cggcggcagc gtaatcggga aaaccgagag tcgataaaac aaatcttttc tgaattttcc
      901 ttcttcgacg gctttttcca gattgacgtt tgaggcggcg atgacgcgca cgtcggtttt
      961 gaccgtttcg tttccgccga cgcgcgtaaa ttcgccgtcc tgcaaaaccc tcaggagctt
     1021 cacctgcgcc gccagaggca tctcgccgat ttcgtccaga aacaaagtgc cgcggtcggc
     1081 ttcttcgaaa cgtcctttgc gctggtttcg cgcgtcggta aacgcgcctt tttcgtgtcc
     1141 gaaaagctcg ctttcgagca gagtttcggg aatcgcgccg cagttcagtt tcacatacgg
     1201 cttgttttcg cgaccggaat tataatgaat cagattcgcc agaagctctt ttcccgttcc
     1261 ggattcgccc tgaatcaaca cggtcgtctg cgtgtcggcg acgtttaaag ccagctcgat
     1321 ggcgcggcga atcgtcggag cctgcccgat aatctcgctt tgatgtttct ggtgaagcgc
     1381 gcttttcaga cgcatcactt ccgccgaaag ctggtcgcgt tcgagcgccg tgccgatttg
     1441 atcggaaatt ccctcgacca acgcgacttc gtgattttca aaaccgccgt cgcgctcctc
     1501 gctccagaca aaacccagaa gtccgaacgc ctgaccgccg acgcgcaccg gcgcgaccag
     1561 cgcggcgcgc ccgtcgagct gcgaattaaa aatcaggcgg acggcaaacg gcaaatcctt
     1621 atcgaccaga cgcagggttt ttccttcgcg caaaatgtcg ctcaaaaccg gaaacggctc
     1681 gatgtcgagc gattgattat gctcttcgat catctgcaaa accgcgttcg gctcgacatt
     1741 cggcgcggat ttaaacgaag ccaggcttat tttttttccc gtctggtcga gcacgccgag
     1801 cgcgccgtaa tttgcgccga caagctcgac ggcgcgttcc agaacgcgag cggaaacctc
     1861 gttaaaatcc gaatgcgaat tgagcgtgtt ggcgatttcc agcagcgcgt tggtgcggcg
     1921 ggtttcctgt tcctgcgcca cataaagccg cgtgtattga tagccgatgg cgagctggcg
     1981 accgaccgat tcgagaaaag cgacttcctc gtcgagccat tcgcgcggcg cgtgcgtgtc
     2041 gtgcaaaccc aggagcccga tgactttttc gttcaaagta atcggcacga cgagcagcga
     2101 ttgcgtttcc agagctttgg taaaaaagcg aacggtcgcg tctttcgtct tggtcgtgtc
     2161 gttgatgcga accggctgcg ataaatcgaa tttttcggtg agcttctgcg cgtcgaacgg
     2221 aatcatcgtg ccttccgacg gcggaacggt ttcgtccttg cgccattcgt ggctgatgcg
     2281 cagctcgttg ccttccgtca gctgcaaaag gtcgcagcgg tcggcgtcca tcatccgccc
     2341 gatttccgat acgacgacgt tcagaaatct ttcgaggtcg atgttggtcg gcaaatcgcg
     2401 cgccagccgg tcgataatgc cttcgcgcgc ctcgtgcatt ttgatttttc tttccagcga
     2461 aagcgattgc ggaatttcca gttcgatttt catacgcgaa ttttgaacta aacctttaaa
     2521 tttaaaagcg cttaattcga atataagttt atcatttttt agcggaattt atgtcaggct
     2581 gacacaggaa attttcccgg tgtgtttttt agatgcaaat aaaaatcttc tgaattctaa
     2641 aatagtgtga aactttttcg aatttttacc aataataatt ggaaagcgtg aaaatctaca
     2701 tttaccgtag aaaaactcaa tggaaaaacc gcgagatgac ggcgagacct cagaatcgct
     2761 cacgaccacg gataatccac cgaatacgaa tttccaggat aaaataaagt aaaaacgagg
     2821 agtaaaaaat gaaaagaatt cttacgcgta tggcggcgct ggcgctgctg gtaattgcga
     2881 ctttctggtt cggcgccgac acgcaagcca aaaaatgggg ctgcgccgac agtatgtttg
     2941 ccgcccaatc cgcctgcgac gacactttat cgggaactat cggcagttat aacaacgtca
     3001 ctaatttctc gcccaatcaa tgcgacaatc aggcgcaggt tcaatgcggc aacaattcga
     3061 cctgttacaa tagcacttat aacaattgct acaacagcgc cgt
//
LOCUS       DASWNE010000269         4541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_323454, whole genome shotgun sequence.
ACCESSION   DASWNE010000269 DASWNE010000000
VERSION     DASWNE010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4541
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323454"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..727)
                     /locus_tag="VGB68_05565"
     CDS             complement(47..727)
                     /locus_tag="VGB68_05565"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEY0048731.1"
                     /translation="MRGITNLPVFPLPVVLLPNELLPLHIFEPRYRQMLKDVQLTNQV
                     FGVSYFSAEESVIEKPETGTLGCAAEVRNVQMLEDGRANIVTVGLIRYRLEGYVETDE
                     PYLIGEISFFEDDEEDASLLQSLADEVFALFMRVAKAAHDLSGERGKMPEIPQVEPQH
                     LSFLITAAFNLQPEVKYEFLEIRSTAERLERLREMLRQTVGRVEESAHLTKIAKTNGH
                     SNKPIRLD"
     gene            complement(928..2355)
                     /locus_tag="VGB68_05570"
     CDS             complement(928..2355)
                     /locus_tag="VGB68_05570"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HEY0048732.1"
                     /translation="MKFLHISDIHLGCTRYQLEESPKDFYRAWIDVLQKYAVGEKVDF
                     VVMAGDFFHKRNVPPETMNHAFAGLTLLKDAGIPVVAIEGNHDQKHNDSEYSWLRSLE
                     NWRLMHLVEPRNENGKFIYEAWDYDSEHYIRGGYIDIGNARVFGSAWYGASANWAIPM
                     LTKALKENRRAGAFHILLLHTDVEGHQTHPIPALSLANLKELKEVTDYVALGHTHKHY
                     EIDNWAFNPGSLEITSIDEYRETRGAFLVEVGENNQITARHVRDYRQRPFQRLSFDVS
                     GCQEAKAVTDGVLEMVSKQGRMMNDERGTMNQYGETPSAERETPNEEGESINDVREMM
                     RAKREMTNSEAEAAMSSFGVYNSSFSLAPIIEITLRGHLGFPNSLLELKTIREEAQQL
                     TGALHVRIKNHSAPVEYAVAADLEEDASREKLERRVIEDLIIRDNRFKTRVENISEAV
                     IGAKRMALSDDEPEKIVDFISMKIA"
     gene            2845..4407
                     /locus_tag="VGB68_05575"
     CDS             2845..4407
                     /locus_tag="VGB68_05575"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0048733.1"
                     /translation="MKILLYNPDNGVTRNFMPHLWMFLLQALTPKEHEVILIDGNAKA
                     MSRQELVEFCRKENIGLVGIGAMTRMVARAYQVADALREAGIKVVMGGPHVTECPDEA
                     LGKDGGIRHADAVALGEADDTWALIVADAARGELKEIYQPETDEKGNDKKPSLQPYPH
                     IPWETLDLEQFSLVPKFFRPYLSKMGDGWGSFHVVPIETGRGCPYGCEFCTVTGFFGD
                     SIRFRTNESVVEELLRLKERAKREKGQISVFFIDDNLAINKKRVKSLLRDIIAAGAQL
                     PWVAQISANLLGDEELVDLIAESGGKWVFIGMESIDPQNMASVNKNFSKPADYKQVLD
                     RLAKRNIYAITSFIFGMDNDTQGVAARTVEQIESWAPGLPVFGQLTPFPATPLYTRLE
                     KEGRLSRPKHWMEFAPFVMAHTPAKISIEEARAETISAWSRSYSPERNAEVLEALKDK
                     PIGYRISHLVSRFFFRGIYFPQMNKRAWMKLFFDNRKPLYSLVKEGAKTYRNARRNKR
                     VSSESMPTTEQI"
BASE COUNT         1073 a         1162 c         1185 g         1121 t
ORIGIN      
        1 gaaagagcta aaataagccg cgcgcctgct gctgctttac ctctaactaa tctaatctaa
       61 tcggcttgtt gctgtgaccg ttggttttgg cgattttggt cagatgggcg ctttcttcga
      121 ctctgccgac ggtttggcgg agcatttcgc gcaggcgttc gaggcgctcg gcggtcgagc
      181 ggatttccag aaattcatat ttgacttccg gctggagatt aaaggcggcg gtgattaaaa
      241 atgaaagatg ctgcggctcg acctgcggaa tttcgggcat tttgccgcgc tcgccgctga
      301 ggtcgtgcgc cgctttcgcc acgcgcataa acagcgcgaa aacttcgtcc gccagagatt
      361 ggagaaggct cgcgtcttcc tcgtcgtctt caaaaaacga aatttccccg attagataag
      421 gctcgtccgt ttcgacatag ccctccagcc ggtagcgaat caagccgacc gtgacgatat
      481 tggcgcgccc gtcttcgagc atctgcacgt tgcgcacttc cgccgcgcag ccgagcgtgc
      541 cggtttcggg tttttcgata accgattctt cggcgctgaa ataagaaacg ccgaaaacct
      601 gattggtcag ctgcacgtcc ttgagcatct gccggtagcg cggctcgaaa atgtgcagcg
      661 gcaaaagctc gttcggcagc aaaaccacgg gcagcggaaa aaccggcagg tttgtgatac
      721 cgcgcattct gtctggggtt tcggacataa tgtaaatatt aaatcgtgaa tcgtgaatag
      781 taaatcgaga aatcgtgaat agtaaatcga gaaatcgtat ttaccgcgcg cgaagattcc
      841 atcttcgccc ggcgcgatgc ccgcgtgaga gattaaacgc cgggcggtac gattcacgac
      901 cggcgatttg ccgtttacta ttcgcgatta agctattttc atcgagataa aatcgacaat
      961 cttttccggc tcgtcgtcgc tgagcgccat tcgtttcgcg ccgatgacgg cttccgagat
     1021 gttttcgacg cgcgttttga aacggttgtc gcgaatgatt aaatcttcga tgacgcggcg
     1081 ttccagcttt tcgcgcgagg cgtcttcttc caaatcggcg gcgacagcgt attcgaccgg
     1141 cgcggaatga tttttgatgc ggacgtgcag cgcgccggtc aattgctgcg cttcctcgcg
     1201 gattgttttc agctccagaa gggaattcgg aaaaccgaga tgaccgcgca gtgtgatttc
     1261 gataatcggc gctaaagaaa acgaggaatt ataaacgccg aacgatgaca tcgccgcttc
     1321 tgcttccgaa ttcgtcattt cgcgttttgc ccgcatcatt tcgcgcacat cgtttatcga
     1381 ttcgccttct tcgttcggcg tctcacgctc ggcgctcggc gtttcgccgt attggttcat
     1441 cgttccgcgt tcatcattca tcattctgcc ttgcttgctg accatttcca gcacgccgtc
     1501 ggtcaccgct ttcgcttcct gacagccgga gacgtcgaac gaaagacgct ggaacgggcg
     1561 ctgtcgataa tcgcggacgt gtcgcgccgt gatttgattg ttctcgccga cctcgaccag
     1621 aaatgcgccg cgcgtttcgc gatattcgtc gatgctggtg atttcgagcg agccgggatt
     1681 aaacgcccag ttatcaatct cgtaatgctt gtgcgtgtgt ccgagcgcga cgtaatcggt
     1741 gacctctttg agctctttta aattcgccag cgacaatgcc ggaatcgggt gcgtctggtg
     1801 accttcgacg tcggtgtgca gcagcagaat gtgaaacgcg cccgcgcgcc ggttctcttt
     1861 cagcgctttg gtgagcatcg gaatcgccca gtttgccgac gcgccgtacc acgccgagcc
     1921 gaagacgcgc gcgttgccga tatcgatgta gccgccgcga atgtaatgct cgctgtcgta
     1981 atcccacgct tcgtagataa attttccgtt ctcgttgcgc ggctcgacca gatgcataag
     2041 ccgccagttt tcgagcgagc gcagccagct gtattcgctg tcgttgtgtt tctggtcgtg
     2101 attgccttcg atggcgacga ccggaattcc ggcgtctttt aacaaagtca gaccggcaaa
     2161 agcgtgattc atcgtctcgg gcggaacgtt tcttttatga aaaaaatcgc ccgccataac
     2221 gacgaaatcg accttttcgc cgaccgcgta tttttgcaga acgtcaatcc aggcgcggta
     2281 aaaatccttc gggctttctt cgagctggta acgcgtgcag ccgaggtgaa tatcggaaat
     2341 gtgtaggaat ttcatagctt tattttatca cagacgagac atctgacata cgggaattca
     2401 aacaggataa acaggataaa aaacttctta tttacaagtt tgaaataaag cttaaacccg
     2461 gaagccggac ggtttgtttt tcagaatcgt gaaaagcgcg gttttgaaat agctaatcgg
     2521 taaaaaaaat gagaatataa acggcaagtc tttgtaaagc ccggtccgat tcaaaaactt
     2581 tccccgttta tgaacaaacg aatgaaaata tctctttatt attacaatta cgcccgtatg
     2641 gttttactgt atttttcgct caatcgatgt tcatatatgt ggaagcacac ttaagtgtgt
     2701 tgcttaagtg tgctacaatg actttcacaa gaatcgcgtt cctgcatcag gtacgaaaat
     2761 aatataaccg agtcgtaaca aaacctgtag acaagcattt tgctgtttat ttctcgattg
     2821 ttaccaagca tagaaagaga atttatgaaa atcctgcttt ataatcccga caacggcgtg
     2881 acgcgcaatt ttatgccgca tttgtggatg tttcttctgc aagccctcac gccgaaagaa
     2941 cacgaagtga ttttgattga cggcaacgcg aaagcgatga gccgccagga actggtcgag
     3001 ttttgccgaa aagaaaatat cggtctggtc ggcatcggcg cgatgacgcg aatggtcgcg
     3061 cgcgcttatc aggtagccga cgctctgcgc gaagccggaa ttaaagtcgt gatgggcgga
     3121 ccgcacgtga cggaatgccc ggacgaagcg ctcggcaaag acggcggaat tcgccacgcc
     3181 gacgccgtcg ctttgggcga agccgacgat acctgggctt taatcgtcgc cgacgcggcg
     3241 cgcggcgagc tgaaagaaat ttatcagccc gagacggacg aaaaaggcaa tgacaaaaag
     3301 ccttctttgc agccttaccc gcacattccc tgggagactc tggatttgga acaattcagc
     3361 ctcgtgccga aatttttccg cccttacctg tcgaagatgg gcgacggctg gggctcgttt
     3421 cacgtcgtgc cgattgaaac gggacgcggc tgtccttacg gctgcgagtt ctgcaccgtg
     3481 acgggatttt tcggcgattc gattcgtttt cgcacgaacg aaagcgtcgt cgaagaactt
     3541 ttgagattaa aagaacgcgc caaacgcgag aaagggcaaa tctccgtttt ctttatcgac
     3601 gacaatctcg ccattaacaa aaagcgcgtc aagtccttgc tcagagacat tatcgccgcc
     3661 ggcgctcaat tgccgtgggt cgcgcaaatc agcgccaatc tgctgggcga cgaagaactg
     3721 gtcgatttga ttgccgaatc cggcggcaag tgggttttta tcgggatgga atccatcgac
     3781 ccgcagaata tggcttcggt caacaaaaat ttctcgaaac ccgccgatta caaacaggtg
     3841 ctcgaccgtc tggcgaaacg caatatctac gcgattacgt ctttcatttt cggaatggac
     3901 aacgatacgc agggcgtcgc cgcgcgaacc gtcgaacaaa tcgaaagctg ggcgcccgga
     3961 cttcccgttt tcggtcagct gacgcctttt ccggcgacgc cgctttatac gcggcttgaa
     4021 aaagagggaa gattatcgcg tccgaaacac tggatggaat ttgccccgtt tgtaatggcg
     4081 cacacgcccg cgaaaatttc aatcgaagag gcgcgcgccg aaacgatttc cgcctggtcg
     4141 cgctcttaca gtccggaaag aaacgccgaa gtcctcgaag cgctcaaaga caagccgatc
     4201 ggctaccgca tcagccatct ggtttcgcgt tttttctttc gcggaattta tttcccgcag
     4261 atgaacaaac gcgcttggat gaaattgttt ttcgataacc gcaagccgct ttacagtctg
     4321 gtcaaggaag gcgcgaaaac ttatcgaaac gcgcggcgca acaagcgagt gtcatcggaa
     4381 tcgatgccga cgaccgagca gatttaagag caaattcgta taaattcaga aaatgtttcg
     4441 gcggtgtaaa aattttacac cgccgattta tttgtcgggt aattgcccgt ttattattat
     4501 cgagccgact ttttgtaaag ctcgattcct tcgtccacaa t
//
LOCUS       DASWNE010000270         4948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_8591, whole genome shotgun sequence.
ACCESSION   DASWNE010000270 DASWNE010000000
VERSION     DASWNE010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4948
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8591"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VGB68_05580"
     CDS             <1..472
                     /locus_tag="VGB68_05580"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0048734.1"
                     /translation="GQSVPNPRPADYDGDGKADIASYNCFPIPGQNPVRCEWAILRSS
                     VNNVRIEQFGNGDDSPLPPADYDGDGKADIAVFRPSERVWYLLQSTAGFAAAQYGLES
                     DIARPSDFDGDGKADITVWRPSDGTWYLLRSAQGFTGIKFGASGDIPVPNSFVR"
     gene            659..814
                     /locus_tag="VGB68_05585"
     CDS             659..814
                     /locus_tag="VGB68_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048735.1"
                     /translation="MANTEEKCAQEMCRCPRANDSDYCSQQCADAADADITGIMCECG
                     HAGCAVS"
     gene            complement(915..1433)
                     /locus_tag="VGB68_05590"
     CDS             complement(915..1433)
                     /locus_tag="VGB68_05590"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEY0048736.1"
                     /translation="MNDNKLALEEAQRTANYEALKSSVKTDVESEIAARADHSTRAEA
                     GQLNNLAGDFRQKAINEVVETDREVERGRGLARVSQVVDYIFYVIYALLAVRLLLALF
                     AARESAGFVQFVKSVTDPFYAPFKGIVPSIRTDDGFTLALPIIVALFVYALIHLGING
                     LLRIFVHRKTAI"
     gene            complement(1434..1661)
                     /locus_tag="VGB68_05595"
     CDS             complement(1434..1661)
                     /locus_tag="VGB68_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048737.1"
                     /translation="MQGSDTPQTKREEWDAEKIADEASNRMSDEIQREVARGDETKGD
                     PDERDVVGGPASIDTPQGREEAKTRAEGETS"
     gene            complement(2113..3723)
                     /locus_tag="VGB68_05600"
     CDS             complement(2113..3723)
                     /locus_tag="VGB68_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048738.1"
                     /translation="MSRIFLNAFTFVLLTITLSTAYTAQGQLRPYRVNDRQVQVLINR
                     IETGTDTFRRQVDRNLDRSTMNGTRTEDNLTSYVTDFENATDSLRRRFDARTSTTADV
                     QEVLNRASMIENFVRDNRLSTNAQRQWTLVKTDLDTLATYYGVRWDWTARINNNDRYD
                     NRDNRGGIGGGVRGGRGDSRLTGTYRLNTSQSDDVNAILERTFGNNTGTDVNQRQRGR
                     RNMERRLTAPDMLAIEKINQRVTIASSLSPQVSFDADNVSRSEVSANGRTVNIRAAQD
                     ANGLTLSYEGDRMNDYNINFVPVGNNQLRVTRKLYLENRGETITVSSVYDKIDQTARW
                     TDMGGSNNTGNTGTINNNNNYDFVVPNGTRLVAVLSTPLTTRTTQNGDRFTMEVTSPT
                     QYRGAIIEGTVGAAERSGRVSGRAELALNFETIRLRSGQTYRFTGLIDSVKNANGDSV
                     GINNEGAVRDGNQTTKTVTRAGVGAALGALIGAIAGGGQGAAIGAGVGAGAGAGSVIL
                     QGRDDLRLDSGTEFNLTSSAPSNLRGSY"
     gene            complement(3937..>4948)
                     /locus_tag="VGB68_05605"
     CDS             complement(3937..>4948)
                     /locus_tag="VGB68_05605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0048739.1"
                     /translation="TDGYFTARTVTDVAGRKWIVEYKALPAYVAESNSGWTPLVFGSG
                     LVFSLLLFGMTYLESFARRKSERFTYELKESEKEKALLFEKEQKARRFAEDASRAKDE
                     FISVVSHELRTPLNTIAGWTRILQSDNLAEEMKQQALEKIDKNLRIQTKLVEELLDFS
                     QIITGKNDLNSKLVEISGVFEQAVAGIAEQADEKEINIEKKNTLNGQKVLGDSEHLKK
                     AFGNVLRNAVKFTSKNGTIFTEIKEREDTIEMKIRDSGRGINPKFLPHIFEGFRQADS
                     SSTRQFGGLGLGLAISRHIIELHGGSIKAESAGEGKGSVFTIKIPVSKDGEPTSALSS
                     ID"
BASE COUNT         1170 a         1280 c         1188 g         1310 t
ORIGIN      
        1 tggtcagtct gttcccaacc cgcgccccgc cgattacgac ggcgatggaa aagccgacat
       61 cgccagctat aactgtttcc cgattccggg acaaaatccc gtgcgctgcg aatgggcaat
      121 cctgcgaagc tcggttaata atgtccgaat tgagcagttt ggaaacggcg atgattcgcc
      181 gcttccgcca gccgattatg acggtgacgg caaagcggac atcgcggttt ttcgcccgtc
      241 ggaacgcgtc tggtatctgc tgcaaagcac cgccggtttc gccgctgcgc aatacggtct
      301 ggaatcggac attgcgcgac cgagcgattt tgacggcgac ggtaaagccg acatcaccgt
      361 ctggcgaccg tcggacggca cctggtatct gcttcgctcg gcgcagggct ttaccggcat
      421 aaaattcggc gcgagcggcg acattcccgt tccgaattca tttgttcgat aaagttttca
      481 aaaaggcgga aacgtgcctg tgaaagaaca cgcttttcaa aagacagcgc gaatttcttt
      541 agagattaac ttaatctcta aagaaattcg ctaatttatt gccttatccg aaactggggt
      601 ctaaaattaa actgttccaa ggaactataa ttttacgaac tcagggagga ttaatttaat
      661 ggcgaatacg gaagaaaaat gcgcgcagga aatgtgcaga tgcccgcggg caaacgattc
      721 ggattattgc agccagcaat gcgctgatgc ggcagacgcc gatattacgg gaattatgtg
      781 cgagtgcggg cacgcaggct gcgcagtcag ttaactagct cgggcagaca cgcgacatta
      841 cggtcgcgtg agcttgagta aaaaatgaaa agcgaatagc aaaaaaacta ttcgcttttc
      901 attttttgat gattttaaat cgctgttttg cgatgcacga aaattctcaa tagcccgttg
      961 attccgagat gaatcagagc gtaaacgaat aaagcgacga taatcggaag cgccagcgta
     1021 aaaccgtcat cggtgcggat gctcggcacg attcccttga aaggcgcgta aaacgggtcg
     1081 gtcacggatt taacaaactg cacgaagccc gcgctttcgc gcgccgcaaa aagcgccagt
     1141 aaaagacgaa ccgccagcaa cgcgtatata acgtagaaaa tataatcgac gacttgcgaa
     1201 acgcgcgcca aacctcgccc gcgctctact tccctgtccg tttcgaccac ttcgttaatc
     1261 gccttctgcc gaaaatcgcc cgccagattg tttagctgtc cggcttcggc gcgcgtcgaa
     1321 tggtcggcgc gggcggcgat ttcggattcg acgtccgttt tgacgctcga tttcagcgcc
     1381 tcgtaattgg cggttcgttg tgcttcttcc aatgcaagtt tgttgtcgtt catttaagat
     1441 gtttctcctt cagctctggt tttcgcttct tcgcgtccct gcggcgtatc aatcgaggcg
     1501 ggacctccga cgacatcgcg ctcgtcgggg tcgcctttcg tttcgtcgcc gcgcgctacc
     1561 tcgcgctgaa tctcgtcgct catccgattg ctcgcttcat cggcaatctt ttccgcgtcc
     1621 cactcttcgc gcttcgtctg cggcgtgtcg cttccctgca taaattattc ctccctgaat
     1681 aaagaaaaat cggtctttgc tggtttcgtt tttagaatga ataagtattt ttacaaatac
     1741 tcgcattctg atatacaaag cccgtgccag attttgcgta tcaaacacat acaaaaagct
     1801 cgtttttaag taaaaaccga cttacaaatc caatttgtaa gttaacaaac cggatttgta
     1861 agcttacaaa tcacgtttgc aagcttacaa atcacgtttg taagttaaca aatcacgttt
     1921 gtaagtctcc aaacggcatt tgtaagttaa cagacgacga ttgtctgctt actgataact
     1981 tttgtaaaca aacaagtatc atctgcaaat atgtattcag cgcttgccga cgatgaaaac
     2041 ggcgtcttat taatcaaaaa acgggtggat caaatttcca cccgttaatc atttaaaggt
     2101 tcttactgct cgttaataac ttcctctcaa gttggaggga gcagaagaag tcaaattgaa
     2161 ttccgtgccg gaatcgagtc gaaggtcgtc gcgaccctgc aagataaccg agccagcgcc
     2221 agcgccagcg ccgacacctg cgccgattgc cgcgccttgt ccgccgcccg caatcgcgcc
     2281 gattaaagct ccgagcgccg cgccgacacc tgcgcgcgtc acggttttgg tcgtttgatt
     2341 accgtcacga acagcgcctt cgttattgat gccgaccgaa tcgccgttgg cgtttttcac
     2401 gctgtcgatt aagccggtaa agcgataagt ctgcccgctg cgcagtcgaa tcgtttcaaa
     2461 attcaaagcc agctcggctc tgcccgaaac gcgacccgaa cgttcggcag cgccgaccgt
     2521 tccttcgata atcgccccgc gatattgcgt cggcgaggta acttccatcg taaatctgtc
     2581 gccgttctga gttgttctgg tcgtcagcgg cgtgcttaaa accgcgacca gacgcgttcc
     2641 gttcggaacg acgaaatcgt aattattgtt gttgttgatt gtgccggtat ttcccgtgtt
     2701 gtttgaaccg cccatatccg tccatctggc ggtctggtcg attttgtcat acacgctgga
     2761 aaccgtgata gtctcgcctc gattttcaag atacagcttg cgagtgacgc gcaattgatt
     2821 gttgccgacc gggacgaaat tgatgttgta gtcattcatc cggtcgcctt cgtagctgag
     2881 agtcaagccg ttagcgtcct gcgcggcgcg aatattgacg gttcgcccgt tagcggaaac
     2941 ttcgctgcgc gagacgttat cggcgtcaaa actcacctgc ggcgataatg acgaagcaat
     3001 cgtcactctc tgatttattt tttcaatcgc cagcatatcc ggtgcggtta agcgtctttc
     3061 catattgcga cgcccgcgct gacgctgatt gacgtcggtg ccggtgttat tgccgaacgt
     3121 gcgttcgaga atggcgttga cgtcatcgct ttgactggta ttcaagcgat aagttcccgt
     3181 taatctcgaa tcgccgcgac cgcctctaac gccgccgccg atgccgcccc ggttatctct
     3241 attatcgtat ctgtcgttgt tgttgattcg cgccgtccaa tcccagcgga cgccgtaata
     3301 tgtcgccaaa gtatccaaat cggttttgac gagcgtccat tgacgctgcg cgttagtcga
     3361 caaacgattg tcgcgcacga aattttcaat catactcgcc cgattcaaaa cttcctgcac
     3421 gtcggcagtc gtcgatgtgc gggcatcgaa gcggcggcgc aggctgtcgg ttgcgttttc
     3481 aaaatcggtg acgtaagaag tcagattgtc ttcggtcctg gttccgttca tcgtgctccg
     3541 gtcgagattg cggtcaacct gtcggcgaaa tgtgtcggta ccggtttcaa tccggttgat
     3601 aagcacctga acctgcctgt cgtttacccg gtacgggcga agctgcccct gcgcggtgta
     3661 agcggtcgaa agtgtaattg tcaataaaac aaaagtaaaa gcattaagga aaatcctact
     3721 cattattaac ctccttgtat tagaaaaacc attggaagag tacaagacgt gccggtctta
     3781 ttgcttaaac aactggcaat cggcgtgcca ccgttcggga ttcagagtaa ctactgtcgt
     3841 cacttcactt taaaaatggt tcctaaaaat tttatgaatt ttatttaatt cgttttgtaa
     3901 gtattcgata caaaactgag tggttttcag agggttttaa tcaattgaag acaaagcaga
     3961 tgtcggctcg ccgtctttcg atacgggaat ttttatggta aaaacagagc cttttccctc
     4021 gcccgcgctt tcggctttaa tcgagccgcc gtgcagctcg ataatgtgac gcgaaattgc
     4081 caagccgagc cccaagccgc cgaattgtcg tgtgctggaa gaatccgcct ggcggaatcc
     4141 ttcaaaaata tgcggcagaa acttcggatt gatgccgcgc ccgctgtcgc ggattttcat
     4201 ttcaatcgta tcttcccgct cttttatctc tgtaaaaatg gttccgtttt tagaagtaaa
     4261 ttttacggcg tttcgcaaaa cgtttccaaa cgcttttttt aaatgttcgg aatcgcccag
     4321 aactttttgc ccgttcagcg tatttttttt ctcgatatta atttcttttt catcggcttg
     4381 ctcggcaatg ccggcaacgg cttgttcaaa cacgccggaa atttcaacta atttcgagtt
     4441 taaatcgttt tttccggtaa taatctgcga aaaatcgagc agttcttcga ccagctttgt
     4501 ttgtatgcgc aggtttttat caattttctc cagcgcctgc tgtttcattt cctccgctaa
     4561 attatccgat tgaagaattc gcgtccagcc ggcaatcgtg ttcagcggcg tgcgcagttc
     4621 gtgcgagacg accgagataa attcgtcttt cgcgcggctc gcgtcttcgg caaagcggcg
     4681 ggctttttgc tccttttcaa acaacaaagc cttttccttt tccgattctt tcagctcgta
     4741 agtaaacctc tccgatttac gacgcgcgaa agattcaaga taagtcatac cgaaaagcaa
     4801 aagactgaaa accagaccgc tgccgaagac taaaggcgtc cagccggaat tggattccgc
     4861 cacgtaagcc ggaagcgctt tatactcgac aatccacttt cgaccggcaa cgtcggttac
     4921 cgtccgcgct gtaaaatagc cgtccgta
//
LOCUS       DASWNE010000271         2110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_256367, whole genome shotgun sequence.
ACCESSION   DASWNE010000271 DASWNE010000000
VERSION     DASWNE010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256367"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1818)
                     /locus_tag="VGB68_05610"
     CDS             complement(<1..1818)
                     /locus_tag="VGB68_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEY0048740.1"
                     /translation="MKLFSKFILLIAFLATASTTVTAGYRVQSGRIYDNSTNQEIQLR
                     GVNWFGFQETEDYIVHGLWLRNWRSQIKQMKNIGVNAVRLPFCPAVLENKPSTKPGII
                     NYSLNRELRYKKSLELFDLVVNELNSEQMYILLDHHTPDCDNLTELWYTDAYSEQKWL
                     DDLAFVAERYKNLPYVIGIDLKNEPHGEATWGTGLATDWKLAAEKAATRVLSVAPDML
                     IFVEGIEDNNQPYVPPCSTGGHWWGGNFEPMDCYPLNIPADRLVLSPHVYGPDVSNHT
                     YFNHPDFPNNMPYWWDLHFGHFAPQYPIVFGEFGGRYGHSSADRVADPRDIPWQNKMV
                     DYMIEKNMRSSFYWSWNPNSGDTGGILQDDWTNVWDDKVALLHRLWNYTPQAPEHGCS
                     DGRDADGDGLLDYPADPGCENAEDDDEFNAPIGSQGLELTTVTNNDWGTGYCKEIRVR
                     NTNGFDIDWLINQTIEGEITSIWSGTYTRNGNEVGFGGTWNDILDAGKETTFGFCVTR
                     PAPPPPACADGQDNDQDGLVDLNDPGCATAQDTDESNAPPSSEGLTTDINIVNDWGGG
                     YCADVTVANTTANPIDWTVSFTIEGTVSGLWNVAYVQNGN"
BASE COUNT          457 a          565 c          544 g          544 t
ORIGIN      
        1 atttccgttt tggacatagg cgacgttcca gagaccggag acggttcctt caatcgtaaa
       61 actcactgtc cagtcaatcg ggttcgcggt cgtattcgcc accgtgacgt ccgcgcagta
      121 accgccgccc caatcgttga cgatattaat atcggtcgtc aaaccttcgc tcgatggcgg
      181 cgcgtttgac tcgtcggtgt cctgcgcggt tgcgcagccc gggtcattca ggtcgactaa
      241 tccgtcctgg tcgttgtcct gaccgtcggc acaggcaggc ggcggaggcg caggacgcgt
      301 aacacagaag ccgaaagttg tctcttttcc ggcatcgaga atgtcattcc acgttccgcc
      361 gaagccgact tcgtttccgt ttcgcgtgta agttccggac cagatactcg taatttcgcc
      421 ttcaatcgtc tggttgataa gccagtcgat atcgaaaccg ttcgtgttcc tgacgcgaat
      481 ttccttgcag tagcccgtcc cccaatcatt gttggtaacg gtcgtcagct ccaagccctg
      541 actgcctatc ggagcattaa attcatcgtc gtcttcggcg ttttcacagc ccggatcagc
      601 cgggtaatcc agcagcccgt cgccgtcggc gtcgcgcccg tcggagcagc cgtgttccgg
      661 cgcctgcggc gtgtaattcc ataagcggtg cagcagagcg actttatcgt cccagacgtt
      721 cgtccagtcg tcctgcaaaa ttccgcccgt atcgccgctg ttcggattcc aggaccagta
      781 aaaactgctt cgcatatttt tctcaatcat gtaatcgacc attttgtttt gccaggggat
      841 gtcgcgcggg tcggcgacgc gatctgcgct gctgtgaccg tatctgccgc cgaattcgcc
      901 gaaaacgatc ggatattgcg gcgcgaagtg tccgaaatgc aaatcccacc aataaggcat
      961 attgttcgga aaatccgggt ggttgaaata ggtgtggttg ctgacgtccg gaccgtaaac
     1021 gtgcgggctt aaaaccagac ggtcagccgg aatattcagc ggataacagt ccatcggctc
     1081 aaagtttccg ccccaccaat gtcctccggt gctgcacggc gggacatacg gctgattgtt
     1141 gtcttcgatg ccctcgacaa aaatcagcat atccggcgcg acggacaaaa cgcgcgtcgc
     1201 ggctttttcg gcggcgagct tccagtcggt cgctaagccc gttccccagg ttgcctcgcc
     1261 gtgcggctcg tttttcaggt cgatgccgat gacgtagggc agatttttat agcgctcggc
     1321 gacaaacgcc aaatcgtcga gccatttctg ctcgctgtac gcgtccgtat accagagctc
     1381 ggtcagatta tcgcaatccg gcgtgtggtg gtcgagcagg atgtacattt gctcgctgtt
     1441 cagctcgttg acgaccaaat cgaaaagctc aagcgatttt ttgtatctca actcgcggtt
     1501 caaactgtaa ttaataattc cgggtttggt cgacggcttg ttttccagaa ccgccggaca
     1561 gaaaggcagg cgaacggcgt taacgccgat gtttttcatt tgctttatct ggctccgcca
     1621 gttgcgcagc cagagcccgt gaacgatgta gtcctcggtt tcctgaaagc caaaccagtt
     1681 gacgccgcgc agctgaatct cctgatttgt gctgttgtcg tagatgcgtc ctgactgcac
     1741 gcgataacct gccgtgacgg tcgtgcttgc ggtggcgaga aatgcaatta gcaatataaa
     1801 tttagaaaat agtttcatag taattctcct ctcacgtatt tattagcgtg cggtttatgg
     1861 gcagcgctta atatgtaacg cgtagaaaaa aatggaaaaa gtatctctga caagaaattt
     1921 ttttttccgg ctgttcgtcg aaattcgatt tttttgaccg tttaacccgc tttccgaccg
     1981 ctcgcagatt tttactaacc gtttagcccg aatcggcgta tattcttaaa aaccaggatt
     2041 tacgagatga atttcgatag caatccgggt ttatcaacga ttaatttcag gaaataaaat
     2101 caaggagcaa
//
LOCUS       DASWNE010000272         2949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_502726, whole genome shotgun sequence.
ACCESSION   DASWNE010000272 DASWNE010000000
VERSION     DASWNE010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502726"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1136
                     /locus_tag="VGB68_05615"
     CDS             <1..1136
                     /locus_tag="VGB68_05615"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0048741.1"
                     /translation="AAKQTILVEDNTIKAVGADVAIPAGATVVDLSKYTVLPGLFDAH
                     SHMCLNVAPGQNQLFYTLSVPTPYRAIEGVANARTMLESGFTTIRDVGNNGNYADISL
                     MMALEKGIVPGPTMIASGRIIAPFGGQYQIQPERRGLSEPEYFFADTRDELRKAIREN
                     VHYGARVIKIVVDDQRYIYSVDDIKFIIEEAAAAGLKVAAHAWTERGTRNAAEAGVAS
                     IEHGMNMSDETLQIAKRNNVVLVGTEFTAKLFEHLGLPAAEAEKAHLAYVERLKRAYK
                     IGVPMVYGSDAFFNVEGETRGEQSLSIIDSFVKAGVANKDLVRYMTTDAARLLGVDSR
                     RGAIRTGLRADIIATPENPLDNIQTLKKVAFVMKNGKVFKKAG"
     gene            complement(1259..2152)
                     /locus_tag="VGB68_05620"
     CDS             complement(1259..2152)
                     /locus_tag="VGB68_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048742.1"
                     /translation="MIRLDSGVHLPLPGRIVVLSGYMFQALMVNGSLPPKITENDRNF
                     VRNFIHLPFPIREGSLLELLRSAECHGIGLQRIDSGDSKGVPPPIIENGVQTGDMLVD
                     KRNNLWIVSRVEKGLATEIVGAPRFNEDGKAEKGFDPADLIFYWRPSVIPRVWFEGQT
                     ADAAEEFVNIVRLWGLEMTPEEEYSLYRPGSFIKVVLGAFGKRMRDLAGKDDEEVLEY
                     SAARGCLPVRKTGMEARLRDIVRLSGGRWALVLRSNPLDLIVQGGGEGEINEEENAAK
                     ILHAVNHEKITHIRRWAQPSP"
     gene            2126..2590
                     /locus_tag="VGB68_05625"
     CDS             2126..2590
                     /locus_tag="VGB68_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048743.1"
                     /translation="MNSAVQPNHIECVITRIGNSLWKYTRYFDSPQFWSNPGVPGSPN
                     VKDVVLKPVPPQPPELPGETLVAYTKRTKYQPQPEEFEMYNLTDDPLELDNLAGKAAH
                     AVTEAALRNLLGEQCAQKRLTPQSGGVNGQPGCAQAENRLWSSLKNMLNVEQ"
     gene            2647..>2949
                     /locus_tag="VGB68_05630"
     CDS             2647..>2949
                     /locus_tag="VGB68_05630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0048744.1"
                     /translation="MRILIVEDNPKMRRLLRVFLSDVADVCECSDADEVLAAYGDWKP
                     TWVVMDVQMARVGGITATRLLKKAFPESRVVMLSEHDDSETRAAALEAGALDYLIKD"
BASE COUNT          719 a          820 c          798 g          612 t
ORIGIN      
        1 ccgccgcgaa acaaacgatt ctcgtcgaag acaatacgat taaagccgtc ggcgctgacg
       61 tcgcgattcc ggcgggcgcg accgtcgtcg acctttcaaa atatacggtt ctgcccggtc
      121 ttttcgacgc tcattcgcat atgtgcctga acgtcgcgcc cggtcaaaat caactttttt
      181 atacattgtc ggttccgacg ccgtatcgcg ccatcgaagg cgtcgccaat gcgcggacaa
      241 tgctggaatc cggtttcacg acgattcggg atgtcggcaa caacggcaac tacgcggata
      301 tatctttaat gatggcgctg gagaaaggca tcgtgccggg tccgacaatg attgcttcgg
      361 ggcgaattat cgctcctttc ggcgggcaat accagattca gcccgaacgt cgcggacttt
      421 ccgaacccga atattttttc gccgacacgc gcgacgagct cagaaaagcc atccgcgaaa
      481 acgtgcatta cggggcgcga gttatcaaaa tcgttgtcga tgaccaaaga tatatttatt
      541 cggtcgacga catcaaattt atcatcgaag aagccgccgc cgccggatta aaagtcgccg
      601 cgcacgcctg gaccgagcgc gggacgcgca acgccgccga agcgggcgtc gcctcaatcg
      661 aacacgggat gaatatgtcg gacgagacgc tgcaaatcgc taaacgaaac aatgtcgtgc
      721 tcgtcggcac ggagtttacg gcaaagctgt tcgagcatct gggcttgccg gcggcggaag
      781 ccgaaaaggc gcatctggct tacgtcgaac gcttaaaacg cgcctacaaa atcggagtgc
      841 cgatggttta cggctcggat gcgttcttta atgtcgaggg cgaaacgcgc ggcgagcagt
      901 cgctttccat tatcgacagt tttgtcaaag ccggagtcgc caataaagac ctcgtccgct
      961 atatgacgac ggacgcggcg cgtctgctcg gcgtcgatag ccgtcgcgga gccatcagga
     1021 cgggtttgag agccgacatt atcgctacgc cggagaatcc gctcgataac attcaaacgc
     1081 tcaaaaaagt cgcgttcgta atgaagaacg gcaaggtttt caaaaaagcc ggttaaccgg
     1141 cgcgggaaaa ttatcaattg tgagagcctg aaaaaaagcc gaaaaactgt tgcgcaaaat
     1201 cagtcactta cctgcaccgc gggcgacgtt ccggtttttc agcgtcggta tattctattc
     1261 agggagaagg ctgagcccag cgccggatgt gtgtaatctt ctcgtgattg acggcgtgca
     1321 gaattttcgc ggcattttcc tcttcgttaa tctctccttc gcctccgccc tgaacgataa
     1381 ggtcgagcgg atttgagcgc agcacaagcg cccatctccc gcctgaaagc cggactatat
     1441 cgcggagcct cgcttccatt ccggttttcc gcacgggcag acagccgcga gcggcgctgt
     1501 attctaaaac ttcttcatcg tctttgccgg ctaaatctct catacgcttt ccgaatgcgc
     1561 cgagaacgac cttgataaag gagccgggac gatagagcga atattcttct tccggcgtca
     1621 tctccaaacc ccacaggcga acaatattaa caaattcttc ggcggcgtcg gcagtttgtc
     1681 cttcgaacca aactctcgga attacgctcg gtctccaata aaaaattaaa tcggctgggt
     1741 cgaatccctt ttccgccttt ccgtcttcgt tgaatcgagg cgctccgaca atttccgtcg
     1801 ccagcccctt ttcgacgcga gatacaatcc acaagttatt tctcttatcg acgagcatat
     1861 cgcccgtttg cacgccgttt tcgataatgg gcggcgggac gcctttcgaa tcgccggagt
     1921 ctatgcgctg gagcccgatt ccgtgacact cggctgaacg cagcaattcc aaaaggctgc
     1981 cttcgcggat aggaaacggc aaatgaataa agtttcggac aaaattgcgg tcgttttcag
     2041 tgattttcgg cggcaaagag ccgttcacca tcagcgcctg aaacatatac ccggaaagaa
     2101 cgacgatgcg tcccggcagc ggtagatgaa ctccgctgtc cagccgaatc acatcgagtg
     2161 cgtcatcacg cgcatcggca acagcctttg gaaatacacg cgctatttcg acagcccgca
     2221 attctggtca aatccgggcg tgcccggctc gccgaatgtg aaggacgtcg ttctgaaacc
     2281 cgtcccaccg cagccgcctg agctgccggg cgagacgctc gtcgcctaca cgaaacgcac
     2341 gaaataccag ccgcagccgg aagaatttga aatgtataac ctgacggacg acccgctgga
     2401 actggacaat ctggcgggca aagcggcgca cgccgtgacg gaagccgcct tgagaaatct
     2461 gctcggcgag caatgcgcgc agaaacgcct gacgccgcaa agcggcggcg tgaacgggca
     2521 accgggctgc gcgcaggctg aaaaccgtct ttggtcttcg ttaaaaaata tgctcaatgt
     2581 cgagcaatag aaatcgaaga ccaaagacca aagaacgtag ctttgataga gagaaacaaa
     2641 cgcgggatga gaatcttaat cgtcgaagac aatccgaaaa tgcgccggct gctcagagtt
     2701 tttctcagcg acgtagctga cgtctgcgaa tgctcggacg cagatgaagt gctagcggct
     2761 tacggcgatt ggaagccgac gtgggtcgtg atggacgtgc aaatggcgcg cgtcggcgga
     2821 attaccgcca cgcgcctgct gaaaaaagcc tttcccgaat cgcgcgtcgt tatgctttcc
     2881 gaacacgacg attcggaaac gcgcgcggcg gcgctcgaag ccggagcgct ggattatttg
     2941 attaaagac
//
LOCUS       DASWNE010000273         5513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_149329, whole genome shotgun sequence.
ACCESSION   DASWNE010000273 DASWNE010000000
VERSION     DASWNE010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149329"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1436)
                     /locus_tag="VGB68_05635"
     CDS             complement(<1..1436)
                     /locus_tag="VGB68_05635"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HEY0048745.1"
                     /translation="MSRLSFVGCRWRTFFVPLSVFLCLISGLNISAQSLYEDAPILIS
                     QPDSTRALTAQPTRRGLDTSVGVFQPSTKTRITVFVTNLKELMDGEGATAFRAEFEDA
                     RHFRYPLEIVSFEQTVERKWVYALTVRVNGQLGDVGDVLMRVTWRGMSSNRVRVAIGH
                     EGGKIQDDPGAVPTPMPDNPPVEESTESLVGLPWTGDRVRFMEQATFGPKASVESRLR
                     RISYPTWIAEQMEEKRAANGAIRYSTFPYPNLPLLPTLPAGNCGTGTACNRDGYTMYP
                     LQRWFYKEALYGEDQQLRRRVSWALSQIWVVSGRETVQPSRLLRYIEILDRNAFGNYR
                     NLMEEMTLNPAMGNYLDMAISTRQSPNENYAREILQLFSIGVFMLNQDGTVKLDGSGN
                     PIPSYDQSTVNGFTKVFTGWTFCEVSGAQCPNRTLNAVNFIDPMIIVNPSNHDPSSKQ
                     VLNYPGSSSIIPAGQTPELDMKLALDSI"
     gene            1676..>5513
                     /locus_tag="VGB68_05640"
     CDS             1676..>5513
                     /locus_tag="VGB68_05640"
                     /inference="COORDINATES: protein motif:HMM:NF040218.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEY0048746.1"
                     /translation="MFSSVYRHRIVKLSVTGFLFLAALIFFAFRQQTPSRAQNEISPE
                     AIRFAENFDGVISPALPVGWTTSVTGANALFTTTVATPDSPPNAVVTNNPATAGSAEI
                     TSPSITLGNLPTRLSFRHLYNTQLQAGFDGGVLEISIAGGAFQDILAAGGSFLSGGYV
                     QAISGNATGNPLVGRQSWTGNSGGIYTLTLVNLPASAANQSIRLRWRYGTDALDSGVG
                     WRIDNIQITEESSAAVVGFSETFDNVTAPQLPQNWTTAVTGTGASPFVTSTTNPSSPP
                     NCVFTNDPATVSTSEIVSPVIRIGANSPKVIFRHFYNSENGFDGGVLEIKIGNGIFQD
                     IISAGGSFVSGGYNRTLSNCCGNLLGGRMAWSGNSTNYVTTEVNLPPVAFRQQVQFRW
                     RHGSDNTQGGTGWRIDSIQVTNAVTGENPNSIAFSNVGVASPYPSEIQISDLPGKVTK
                     VSVNVTNFTHTAPDDVDLMLVSPSGRSIVLMSDAGGNNGVSNLNLSFEDIAPESLPDN
                     TTLTSGNYKPTDFDAGDSFPAPAPQSAPTGTKLSAFFNDQPNGAWKLFAVDDTGANVG
                     DISGGWSIVIDTSTTAIGIPAVGTSEPYPSEITISGQSGLVSKVTVGLENFSHSSPDD
                     VDLLLVAPNGRKVVLMSDVGGANEVNNVSLTFDDAAVATLPDNTALASGTFKPTDFEA
                     SDAFPAPAPQGTVTGRMLSALNGSEPNGAWQLFLVDDNGNNTGAINAWTLNVQTSAEA
                     ILIPASGSAEPYPSSLAITGQQGSVTKVTVTLNNFSHTSPDDADLLLVAPNGRRIVLM
                     SDVGGNTEVGGLNLIFDDAAAGNLPDTAPLASGTFKPTDFEPGDAFPAPAPSGVTGTT
                     LNAFYGSAANGVWKLYVVDDTGENLGSIAGSWSLNIQSSVSACLFNLSSSVQAFPIGG
                     GSGVFSVLMPAGCPWTASSGSGFISLTAGTSGEGDGAVAFSVGANQGPARTGTIVVTN
                     GAFSRTFQVQQASGCPFAVNQTTMNFSAAGGVGNIQVSAGAECSWQTSADAGWIFVTS
                     APQSGNGTAAFTVQPNPSAARSASVIIGAQTITINQAGAAAKRFDFDGDARADISVFR
                     QGNWYLLQSSAGFTAAQFGLATDLPVPADYDGDRKTDIAVYRQGDWYILQSSNNQFRA
                     QQFGLATDKPVPADYNGDGRSELAVYRQGDWYTLDLTNNQSGSIHFGNETDKPVAADY
                     DGDGKTDQAVYRDGVWYLLRSTEGFTSVQFGIASDKPVVADYDGDGKADQAVYRNGNW
                     YINRSLDGQFSGTQFGL"
BASE COUNT         1198 a         1581 c         1560 g         1174 t
ORIGIN      
        1 aagatgctat cgagcgccag tttcatatcg agctcgggag tttgcccggc agggataatc
       61 gaggacgagc ccggataatt gagaacctgt ttggagctcg ggtcgtgatt cgacggattg
      121 acgattatca tcgggtcgat aaagttcacg gcgtttaaag ttcggttcgg gcattgcgcg
      181 cccgacacct cgcagaatgt ccagccggtg aaaactttcg taaaaccgtt gacggtagac
      241 tggtcgtaac tcggaatcgg gttgccgctt ccgtcgagtt tgaccgtgcc gtcctgattg
      301 agcataaaga cgccgataga aaacagctgc aaaatctcgc gcgcgtaatt ttcattcggg
      361 ctctgccgcg tgctgatcgc catatccaga taattgccca tcgccggatt cagcgtcatc
      421 tcttccatca ggttgcgata gttgccgaaa gcgttgcggt cgagaatttc gatataacgc
      481 agcaggcgcg acggctggac ggtttcgcgc ccggatacaa cccagatttg actcagcgcc
      541 cacgaaacgc ggcgtctgag ctgctggtct tcgccgtaga gagcttcctt gtaaaaccat
      601 ctttgcagcg gatacatcgt gtatccgtcc cggttacaag cggttcccgt tccgcagttg
      661 ccggcgggaa gcgtcggcag cagcggcagg ttcgggtacg gaaacgtcga ataacggata
      721 gcgccgttgg cggctcgttt ttcttccatc tgctcggcaa tccacgtcgg gtagctgatg
      781 cggcgcagac gggattcaac cgaggctttc ggaccgaaag tcgcctgttc cataaagcgc
      841 acacggtcgc ccgtccacgg caagccgacc agcgattcgg tcgattcttc gacgggcgga
      901 ttatccggca tcggcgtcgg cacggctccg gggtcgtcct gaatttttcc gccctcgtgc
      961 ccgatggcga cgcgcacgcg gttgctcgac attccgcgcc acgtgacgcg catcaaaacg
     1021 tcgccgacgt cgccgagctg accgttgacg cggacggtca gcgcataaac ccatttgcgc
     1081 tctaccgtct gttcaaacga aacgatttcc agcggatagc ggaaatgccg cgcgtcttcg
     1141 aattcggcgc gaaaagccgt tgcgccttcg ccgtccatca actctttcag atttgtgacg
     1201 aacacggtaa tccgcgtttt cgtcgatggc tgaaaaacgc cgaccgaagt atccagcccg
     1261 cgcctggtcg gctgcgccgt cagagcacgc gtcgaatccg gctggctgat taaaatcggc
     1321 gcatcttcat acaaagactg cgccgaaata ttcaaaccgg aaattaaaca caaaaaaacg
     1381 ctcagcggca caaaaaacgt gcgccagcgg caaccgacaa aagaaagtcg tgacatcgaa
     1441 ttttcctccg aatctcttca gacgagctga atcagaaact cttgattggg actatacgga
     1501 ataacagttg ggcaacaaga ataaagactt gtatacctct ttttgagtaa aaaagcaagt
     1561 tttccgaaaa aaatgttgac aaattttacc gctggctctt actcttgatt aaatcgcaag
     1621 tccgcatccc ccataagcgc ccaaaattct atgaggtcaa ggtcagggta tgagaatgtt
     1681 ttcttccgtc tatcgtcacc gcatcgttaa actttccgtc accggttttt tatttcttgc
     1741 cgcgctgatt tttttcgctt tccggcagca gacgccgagc cgcgcgcaaa atgaaatcag
     1801 cccggaggcg attcgctttg ccgaaaattt cgacggcgta atttcgccgg cgcttcccgt
     1861 cggctggacg acatccgtca ccggagccaa cgcgcttttt acaacgaccg tcgccactcc
     1921 cgattcgccg ccgaacgcgg ttgtgacaaa taatccggcg actgctggtt cagccgaaat
     1981 cacctcgccg agcatcacgc tcggcaatct tcccacccga ttgtctttcc ggcatcttta
     2041 taatactcag ctgcaggcgg gatttgacgg cggcgtgctg gaaatcagca tcgcgggcgg
     2101 cgcgtttcag gacattctgg cggcgggcgg aagtttttta agcggcggat atgtgcaggc
     2161 gattagcggc aacgcgacgg gcaatccgct tgtcgggaga cagtcttgga cgggaaactc
     2221 gggaggaatt tacacactga cgctcgtcaa tttaccggcg agcgccgcca atcaaagcat
     2281 cagactgcgc tggcgatacg gaaccgacgc gctcgacagc ggcgtcggct ggcgaatcga
     2341 caacattcag ataaccgaag aatcaagcgc ggcggtcgtc ggattttcag aaactttcga
     2401 caatgtgacc gcgccgcaat tgccgcaaaa ttggacgacg gctgtcacgg gaacgggcgc
     2461 ttcgccgttc gtcaccagca cgacgaatcc gagcagcccg ccgaactgcg tttttacaaa
     2521 cgaccccgcc accgtctcca cctccgaaat cgtttcgccg gtcattagaa tcggagccaa
     2581 ctcgccgaaa gtaattttca gacatttcta taactccgaa aacggctttg acggcggcgt
     2641 gctggaaatc aaaatcggaa acggaatttt tcaggacatc atcagcgcgg gcggctcgtt
     2701 cgtcagcggc ggctacaacc gcactctgag caattgctgc ggaaatctgc ttggcggacg
     2761 gatggcttgg tcgggaaaca gcaccaatta cgtgacgacg gaagtcaatc tgccgcccgt
     2821 cgctttccgg cagcaggttc aatttcgctg gcggcacggt tcggacaaca cgcagggcgg
     2881 tacgggctgg cgcatcgaca gcattcaggt gacaaatgcc gtgacgggcg aaaacccaaa
     2941 ctcgattgct ttctcgaacg tcggcgtcgc ttcgccgtat ccgagcgaaa ttcaaatttc
     3001 ggatttgccc ggcaaagtaa ccaaagtcag cgtaaacgta acgaatttta cgcatacagc
     3061 gcccgacgac gtcgatttga tgctcgtctc gccgagcggt cgaagcatcg ttttgatgtc
     3121 tgatgccggc ggcaataacg gcgtcagcaa cctgaattta tctttcgaag acatcgcgcc
     3181 tgaatcgctg cccgataata cgacgctgac gtccggaaat tataaaccga ccgatttcga
     3241 cgcgggcgat tcatttcccg cgcccgcgcc gcagagcgcg ccgacgggaa cgaagctgag
     3301 cgcatttttc aacgaccagc cgaacggcgc ctggaaatta tttgccgttg acgatacggg
     3361 cgcgaatgtg ggcgatattt ccggcggctg gagcatcgtc atcgacactt cgacgaccgc
     3421 catcggcatt cccgccgtcg gaacgtccga gccttatcca tcggaaatta cgatttccgg
     3481 tcaatcgggt ttggtttcca aagttaccgt cggcttggaa aatttcagtc acagctcgcc
     3541 ggacgacgtc gatttactgc tggtcgcgcc gaacgggcgg aaagtcgtct tgatgtcgga
     3601 cgtcggcggc gcaaatgagg tcaataacgt atctctgact tttgacgatg ccgcagtcgc
     3661 tactctgccc gacaacaccg ctctggcgtc gggaactttc aagccgaccg attttgaggc
     3721 gagcgacgct tttcccgcgc ccgcgccgca gggcacagta accggcagaa tgctctccgc
     3781 tctgaacggc agcgagccga acggcgcgtg gcagttgttt ctagtcgatg ataacggcaa
     3841 taacacgggc gcgattaatg cctggactct caacgtccaa acttcggcgg aggcgatttt
     3901 gattccggca agcggctcgg cggagccgta tccttccagt ctggcgatta ccggtcaaca
     3961 gggctcggtc actaaagtga cggtgacttt gaataacttt agccacacct cgccggacga
     4021 tgccgatttg ctattggtcg cgccgaatgg tcggcgaatc gttttaatgt cggacgtcgg
     4081 cggaaatacg gaagtcggcg ggcttaattt aattttcgac gatgccgccg ccggaaatct
     4141 gccggacact gcgccgctgg cgtccggtac tttcaagccg accgattttg aaccgggcga
     4201 cgcttttccc gcgcccgcgc cgtcaggcgt gacgggcacg acgctcaacg cgttttacgg
     4261 cagcgcggcg aacggcgtct ggaaattata tgtggtcgac gatacgggcg agaatttagg
     4321 cagtatcgcg ggcagctgga gcttgaatat ccaaagctct gtttccgcct gtttgtttaa
     4381 tctctcgtcg agcgtgcagg cgtttccgat cggcggcggc agcggcgttt tcagcgtgct
     4441 gatgccggcg ggctgcccct ggacggcttc gagcggctcc ggtttcatct ctctgactgc
     4501 cggcacgagc ggcgaaggag acggtgcggt ggctttctcg gtcggagcga atcaaggacc
     4561 ggcgcgcacc ggaacgattg tggtgacgaa cggggcattt tcgcgtactt ttcaggtgca
     4621 gcaggcatcg ggctgcccat ttgccgtcaa tcaaacgacg atgaatttct ctgccgcggg
     4681 cggcgtcggc aacattcagg tcagcgccgg agccgaatgc agttggcaaa catcggctga
     4741 cgccggctgg attttcgtca ctagcgcgcc gcaaagcggc aacggcacag ccgcttttac
     4801 cgtccagccg aatccgtccg ccgcccgcag cgcttcggtg attatcggcg cgcagaccat
     4861 taccatcaat caggcgggag ccgccgccaa acgctttgat ttcgacggcg acgcgcgcgc
     4921 cgatatttcc gtcttccggc agggcaactg gtatctgctg caatcgagcg ccggatttac
     4981 cgccgcccaa ttcggtctgg caaccgattt gccggttccg gcggattacg acggcgaccg
     5041 caaaaccgat atcgccgttt atcgccaagg cgactggtat atcctgcaaa gctcgaacaa
     5101 ccagtttcgc gcccaacagt tcggtctggc gaccgataag ccggttcccg ccgattacaa
     5161 cggcgacggg cgctccgaat tggcggtcta tcgccaggga gattggtata cgctggattt
     5221 gaccaataat caatcgggct cgattcattt cggcaatgaa acggacaagc ccgtcgctgc
     5281 cgattacgac ggcgacggca aaaccgacca ggcggtctac agagacggcg tctggtatct
     5341 gctgcgctcg accgaaggct tcaccagcgt gcagttcggc atcgcttcgg ataagcccgt
     5401 cgtcgccgat tacgacggcg acggcaaagc cgaccaggcg gtttatcgaa atggaaactg
     5461 gtatatcaat cgcagtctgg acggacagtt ctccggcacg cagttcggct tgg
//
LOCUS       DASWNE010000274         3030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_467510, whole genome shotgun sequence.
ACCESSION   DASWNE010000274 DASWNE010000000
VERSION     DASWNE010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467510"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="VGB68_05645"
     CDS             <1..838
                     /locus_tag="VGB68_05645"
                     /inference="COORDINATES: protein motif:HMM:NF014116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HEY0048747.1"
                     /translation="RRGKTDEAFAEIRKAVESDPAFAGPAISIAWQIFDGDLARISQA
                     VGDSAQAKSLLSAFLAREKRFDEAFQTWNSLSEVEKQKTYGQSSDELLKQLLQAKKFR
                     AALDVQTQMAAPDSEKPVPDKITNAGFEAAVNTGNASGFEWQIAGGAQPAIGVDDKQK
                     TGGNFSLVIAFNSPNGQDFRSVQQTVVVSPNKTYKFEVSARSDLKTSTSVKWEIVDAA
                     DGKILAATQAVPGNSDWSKLTATFTAPSSEAVTLRLARTACSTPVCPISGRVWFDDFK
                     LE"
     gene            858..2276
                     /locus_tag="VGB68_05650"
     CDS             858..2276
                     /locus_tag="VGB68_05650"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY0048748.1"
                     /translation="MIRKSPRNAHLSSTEQLTTALAPKNLAHTSGIIFFLLAATLVFA
                     PVAFGAVDFWALGILTLIAFLIALLWLFDAWKTGEFSFNTNRLQIPLVGFILIGLIQL
                     LPLRAADVAAGALSISAAASLSLDPYTTRFAVVQLGVYFIFFAAALTFIDTAQRLKKI
                     VLLIIIFGALMAFFGILQRLANPEGIYGVRLTPYAVPFGSFVNQHHFAAFMEMTIGVT
                     LGLLYNKSTKKDKRLLLVIAVVIMGIALIFTSSRGGILSLFGVIGFVTLVSFLRRKTD
                     EDDEAEKSSFQSKFALIGGGLALILVLFGSILLLGGGSSLTRGIGFISQSDISNGRSH
                     FWSVTLQIIRDYPIFGAGFNAYGVAFTRYDTWNGSLRIEQAHNDYLQIFADAGIFGFA
                     CAIAFVYFLFRQGLEKINQTTDRFRRGVAVGALAGCFGILLHSFFDFPLRTPSNAFYF
                     LILVTLATVSVSTAKFKLQDGR"
     gene            complement(2375..>3030)
                     /locus_tag="VGB68_05655"
     CDS             complement(2375..>3030)
                     /locus_tag="VGB68_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048749.1"
                     /translation="MKKLIFFTVFICLTVSLTSAQKRQTAVNPAPKKIVQPTRAGEIP
                     EADWLKMADAMYLEKWEKASALAAQNLQKLKTDNAKKQLAQLRYIYLYTLAGKVSEGK
                     MTFAQLESVANTFVGQEFLLVSRQLLADCNTKVNYICAARDNERVLRVTATNRTGTAI
                     HLFEYVVLGERFDLRANSEKLAFVGGNLKKVESSLYKPDMKIMRLIFDKGYVKVATD"
BASE COUNT          647 a          790 c          812 g          781 t
ORIGIN      
        1 gcggcgcggg aaaaccgacg aagcttttgc cgagattcgc aaagcggtcg aaagcgaccc
       61 ggcttttgcc ggtccggcaa tctcgattgc gtggcagatt tttgacggcg acctggcgcg
      121 gatttcgcaa gccgtcggcg attcggcgca ggcaaaatcg ctgctttccg cttttctggc
      181 aagagagaag cgctttgacg aagcctttca gacgtggaat tcgctttcgg aagtcgagaa
      241 gcagaaaact tacgggcaaa gcagcgacga gcttttaaag cagttgctgc aagcaaaaaa
      301 atttcgcgcc gctttagacg ttcagacgca gatggctgcg cccgattcgg aaaagcccgt
      361 gcctgataaa attaccaacg ccggttttga agcggcggtc aacaccggca atgcaagcgg
      421 ttttgaatgg caaatcgccg gcggcgcgca gccggcaatc ggcgttgacg ataaacaaaa
      481 aaccggcggc aatttcagcc tggtcatcgc tttcaattcg ccgaacggtc aggattttcg
      541 ctccgttcag caaacggtcg tcgtatcgcc gaataaaacg tataaattcg aagtctctgc
      601 gcgcagcgat ttgaaaactt cgacctctgt gaaatgggaa atcgtcgatg cggcggacgg
      661 aaaaattctg gcggcgacgc aagccgtgcc gggcaattcg gactggtcga aattgacggc
      721 gacttttacg gctccgtcga gcgaagccgt cacgctccgg ctggcgcgga cggcttgcag
      781 cacgccggtt tgcccgattt cgggaagagt ctggtttgac gatttcaagc tggaataatg
      841 cggcaatcag caaataaatg ataagaaaat cacccagaaa tgctcatctg agctcaaccg
      901 agcagctgac gacggcgctg gctccgaaaa atctggctca cacgagcggt attattttct
      961 ttctgctcgc ggcaacgctc gttttcgcgc ccgtcgcttt cggcgcggtc gatttctggg
     1021 ctttgggaat tctgactctg attgcttttc tgatagctct attgtggctt ttcgacgcgt
     1081 ggaaaacggg cgaattttcc tttaatacaa accgtctgca aattccgctc gtcgggttta
     1141 ttctaatcgg cttaatccaa ctgcttccgc ttcgcgcggc ggatgtcgcc gccggagcgc
     1201 tttcgatttc cgccgccgct tcgctttcgc tcgacccgta tacgacgcgc tttgccgtcg
     1261 tccagctggg cgtctatttt atttttttcg cggcggcttt gacgtttatc gacaccgcgc
     1321 agcggctgaa aaaaattgtt ctgctcataa tcattttcgg cgcgctgatg gcgtttttcg
     1381 gcattctgca gcggctcgcc aatcccgaag gcatttacgg cgtgcgactg acgccgtatg
     1441 ccgttccgtt cgggtcgttt gtcaaccagc atcatttcgc cgcctttatg gaaatgacca
     1501 tcggcgtcac gctcggcttg ctttacaata aatcgacaaa gaaagataag cgtctgctgc
     1561 tcgtgattgc ggtcgtgatt atgggaatcg cgctgatttt tacaagctcg cgcggcggaa
     1621 ttttaagtct tttcggtgtt atcggattcg tcacgctggt cagttttttg cgccggaaaa
     1681 ccgacgaaga cgacgaagcc gagaaatctt cgtttcaaag caaatttgcg ctcatcggcg
     1741 gcggtctggc attaattctg gttttattcg gctcgattct gctgctcggc ggcggttcct
     1801 cgctgacgcg cggaatcggt tttatcagcc agtcggacat cagcaacggg cgcagccatt
     1861 tctggagcgt cacgctgcag attatccgcg attacccgat tttcggcgcg ggatttaacg
     1921 cttacggcgt cgctttcacg cgttacgata cgtggaacgg cagcttgcgc atcgaacagg
     1981 cgcacaacga ttatctacag attttcgccg acgcgggaat tttcggtttc gcctgcgcca
     2041 ttgcttttgt ttattttctg ttcaggcagg gattggaaaa aatcaatcag acgaccgacc
     2101 gtttccggcg cggcgtcgcc gtcggcgcgc tggctggctg tttcggaatt ctgctgcaca
     2161 gctttttcga tttcccgctc cgcacgcctt caaacgcttt ttattttttg attctggtaa
     2221 cgctcgccac cgtctccgtc agcactgcaa aattcaaatt gcaggacgga aggtagaacg
     2281 cgaaaatgga agaagaccga agacggcaga cggcggaaac ttatcttctg aattctaccg
     2341 tccgtcttcc ggattttgca ctttgcatta gaaatcagtc ggttgcgact tttacgtagc
     2401 ctttgtcgaa aatcagacgc attatcttca tatccggctt ataaagactg ctttcgactt
     2461 ttttcagatt tccgccgaca aaggcgagtt tttcgctgtt cgcgcgcagg tcgaatcgtt
     2521 cgccgagcac gacgtattca aacagatgaa tcgccgttcc cgtgcgattc gtcgccgtca
     2581 cgcgcaaaac gcgctcgttg tccctggcgg cgcagatgta gttgaccttt gtgttgcagt
     2641 cggctaaaag ctggcggctg acgagaagaa attcctgccc gacgaaagtg ttggcgacgc
     2701 tctcaagctg ggcgaaagtc attttccctt ccgaaacttt tcccgccagc gtgtaaagat
     2761 aaatataacg aagctgcgcg agctgttttt tcgcgttgtc ggttttgagc ttttgcagat
     2821 tttgcgcggc gagcgcggaa gctttttccc acttttccag atacatcgcg tccgccattt
     2881 tcagccagtc ggcttcggga atttcgccgg cgcgggtcgg ctgaacaatc tttttcggcg
     2941 cgggattaac agccgtctga cgcttttgcg ccgaggttaa ggaaacggtc agacaaatga
     3001 aaacagtaaa gaaaatcagt tttttcataa
//
LOCUS       DASWNE010000275         1795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_432742, whole genome shotgun sequence.
ACCESSION   DASWNE010000275 DASWNE010000000
VERSION     DASWNE010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432742"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(238..897)
                     /locus_tag="VGB68_05660"
     CDS             complement(238..897)
                     /locus_tag="VGB68_05660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEY0048750.1"
                     /translation="MLKAKLLSSPIEEIANTVTHGFGLFLSIIGFIILVWLASTGGGA
                     WHITGSVIYGSSLVILYAASTFYHGATSPKLKQTLQIVDHCCIYLLIAGSYTPITLIV
                     LRGAFGQSLFAFVWAFAVAGILLKVFFGKRFMIASVFSYLLMGWIGVVAIQPLFAALG
                     FMPIALIVAGGIAYSLGVIFFAWKGFRHHHAIWHLFVLAGSICHFLAIAIYVIPYAVK
                     V"
     gene            complement(917..>1795)
                     /locus_tag="VGB68_05665"
     CDS             complement(917..>1795)
                     /locus_tag="VGB68_05665"
                     /inference="COORDINATES: protein motif:HMM:NF015882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HEY0048751.1"
                     /translation="LNTKDPHEIVQTIKNIAPAFGGINLEDISAPRCFEIEDRLKEEL
                     DIPVFHDDQHGTAVVVLAALINALKIVGKKMEDIKLVVNGVGAAGVACTKIVMAAGVG
                     NIVGCDQSGAIYEGRRENMNWVKDWYARNTNREMEKGSIHDVIRGADVFFGLSVPGVI
                     DARDLDNMAKDPIVFAMANPVPEIMPEEAAGHVAVMATGRSDYPNQINNVLCFPGIFR
                     GALNCRASRINEEMKLAAAHAIAEIIGEDELYPDYIIPSVFDKRVGEAVASRVEEAAY
                     QTGVARRERTDSESKF"
BASE COUNT          440 a          469 c          476 g          410 t
ORIGIN      
        1 cgcgcggagc tggacgaaat ctttaagttt atgaatcagg cttttagata aaacgagcag
       61 ggaatatttc accgcagaga ggcagagatt tttaagaaga aattaaccga ggataaaggg
      121 gatttacagg gataataaag aaaggataaa taatgatttc tttatatctc cttcatcctc
      181 ttcatcctcg gttaatttct ttctctgtgt tctctgtggc taattttctt tgcccaatta
      241 aactttgacg gcgtaaggaa tgacgtaaat cgcgattgcc agaaaatgac agatgcttcc
      301 ggcgagcacg aaaagatgcc agatggcgtg atgatgccgg aagcctttcc aggcgaaaaa
      361 aatcacgccc agcgaataag cgattccgcc cgcgacaatc aatgcaatcg gcataaagcc
      421 cagagcggca aacaaaggct gaatggcgac gactccaatc cagcccatca gcagataaga
      481 gaaaaccgac gcaatcataa accttttgcc gaaaaaaacc ttcagcagta tcccggcgac
      541 ggcaaaagcc cagacgaaag caaacaagct ctgtccgaac gcgccgcgca aaactattaa
      601 ggtaatcgga gtgtagcttc cggcaatcag gagatagata cagcaatggt cgacgatttg
      661 gagcgtttgt ttgagtttcg gcgaagtcgc gccgtgatag aaggtcgaag cggcatacaa
      721 aataaccagc gacgagccgt agatgacgct gcccgtaatg tgccaggcgc cgccgcccgt
      781 gctcgccagc cagaccagaa taataaaccc gataatgctt agaaataatc cgaaaccgtg
      841 cgtgacggtg ttggcgattt cttcgatggg cgatgacagg agtttggctt tcagcattat
      901 tgtctttgtt cgatgctcaa aacttcgatt cgctgtccgt tctttcgcgc cgcgcgacgc
      961 ccgtctgata cgccgcttct tcgactctcg acgcgaccgc ttcgccgacg cgtttatcga
     1021 acacggacgg gatgatgtaa tcggggtaaa gctcgtcttc gccgatgatt tcggcaatcg
     1081 cgtgggcggc ggcgagcttc atttcctcgt tgatgcgcga ggcgcggcag ttcagcgcgc
     1141 cgcgaaagat gccgggaaag cagagaacgt tgttgatctg gttcgggtaa tcgcttctgc
     1201 ccgtcgccat cacggcgacg tgaccggcgg cttcttccgg cataatttcg ggaaccggat
     1261 tcgccatcgc gaaaacgatc gggtctttcg ccatattgtc caaatcccgc gcgtcgatca
     1321 cgcccggaac cgacaagccg aaaaaaacgt cggcgccgcg gattacgtcg tgaatcgagc
     1381 ctttctccat ctcgcggttc gtgttgcgcg cgtaccagtc cttgacccag ttcatatttt
     1441 cccggcgacc ttcgtaaatc gcgcccgact ggtcgcagcc gacgatattt ccgacgcccg
     1501 ccgccattac aatcttggtg caggcgacgc ccgccgcgcc gacgccgtta acgactaatt
     1561 tgatgtcttc catttttttg ccgacaattt tcagcgcgtt gataagcgcg gcgagcacga
     1621 cgacggcggt tccgtgctgg tcgtcgtgaa aaacgggaat gtcgagctct tccttcaggc
     1681 ggtcttcgat ttcaaaacag cgcggcgcgg aaatgtcttc gagattaatg ccgccgaaag
     1741 cgggagcgat atttttgatg gtctggacaa tctcgtgcgg gtctttcgtg ttcag
//
LOCUS       DASWNE010000276         2603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_361053, whole genome shotgun sequence.
ACCESSION   DASWNE010000276 DASWNE010000000
VERSION     DASWNE010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361053"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VGB68_05670"
     CDS             <1..773
                     /locus_tag="VGB68_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048752.1"
                     /translation="LIPSDCIVRDNWMNKPLNWREEGWDVKNLFEIKNGWRIRIENNL
                     LTNNWGSAQDGTAIVIKSTTDSGAGATAADIVFINNVVRGAANALSVNGSEEKGGHQL
                     IIRNNIFDDIDGAKWNGQGFFMKSADWNGLIIENNTIIQTGNAANAYGVVRGFVFRNN
                     VLFEGEYGFKGDGTASGRQTIDRYFPGGDVSFNAIIGGDGSLYRGKNMYPSSLRQLGF
                     VNFESRDFTLRSDSSLRGKGFQGKNIGADLDARTVGGK"
     gene            complement(897..2414)
                     /locus_tag="VGB68_05675"
     CDS             complement(897..2414)
                     /locus_tag="VGB68_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048753.1"
                     /translation="MISRTRLFLIILVTAVFLSLAAASSLTNRPQIDEGMFASPALNL
                     ADKGHFGTTVLETENSPLTRIDERTYWVMPLFLINVSASFKAFGAGIFTMRLVSIFWG
                     LVLLAAWYFIVLKLSGNRNIAFICLALLACDYTILDTASSARMDMMSAALGFAAAAIY
                     LHFRERNLLLAVALSQTLVVLNGLTHPNGILAFFALIFLTLYLDFRRLSVKHLAVAAL
                     PYLIGGAGFGYWVLQDPQAFRDQFIDNALMSGRTSGFSSPVQAIWREFTDRYPHAYGL
                     GYTSSGHGEFVRLKSLILIGYVAGLLGVIFTQSLRRKYFALLVMWAIYFVIMAILDGQ
                     KETPYLVHIVPFYVAFLAITLNELWQKKSLPKPLLAAALVLFVALQMGGMTLRIRKNT
                     YANLYQPTIDYLRQNAGERDSIMGGAGLGFGLNFSENFIGDGKYGLTSGKRPKYIVYS
                     DDTHLSWIESKVFYPEFYEYLPRLLDEQYEVVYENEGYKVYARRQPETPTLAASD"
BASE COUNT          700 a          658 c          710 g          535 t
ORIGIN      
        1 ggttgattcc gtccgattgt atagtgcgcg ataattggat gaataaacct ttgaactggc
       61 gcgaagaagg ctgggacgtc aagaatttgt ttgaaataaa aaacggttgg cgcatacgca
      121 tcgaaaacaa tctgctgacg aacaactggg gaagcgcgca ggacggcacg gcaatcgtca
      181 tcaaatcgac caccgacagc ggcgcgggcg cgactgccgc cgatatcgtt tttattaaca
      241 acgtcgtgcg cggcgcggca aacgccttga gcgtcaacgg ctcggaagaa aaaggcgggc
      301 accagctgat tattcgcaat aatattttcg acgacatcga cggcgcgaaa tggaacggtc
      361 agggattttt tatgaaatcc gccgattgga acgggcttat tattgaaaac aatacgatta
      421 tccagaccgg aaacgccgcg aacgcttacg gcgtcgtgcg cggattcgtg tttcgcaaca
      481 acgttttgtt tgaaggcgaa tacggtttta aaggcgacgg cacggcgtcc ggcaggcaga
      541 cgattgacag atattttccc ggcggcgacg tttcgtttaa cgcgattatc ggcggcgacg
      601 gctcgctcta tcgcgggaaa aatatgtatc cgtcctcgct tcgccagctc ggtttcgtga
      661 attttgaatc gcgcgatttt actttgcgct ccgacagctc tttgcgcgga aaaggttttc
      721 agggaaaaaa tatcggcgcg gatttggacg cgcggacggt cggcgggaaa taaaagggta
      781 aaggataaag gataaaggat aaagaaagaa taaacttttc tttctttatc ctttaccgtt
      841 gggaaggcgt ttatcacgct ggcgttatgc ctctaaaagc cgcttctaaa ggtcgattaa
      901 tccgaagccg cgagcgtcgg cgtttcgggc tggcgtctgg cataaacctt gtagccttcg
      961 ttctcgtaaa cgacctcgta ttgctcgtcg agcaggcgcg gcagatactc gtaaaattcc
     1021 ggatagaaca ctttcgattc aatccacgaa agatgcgtat cgtcgctata gacgatatat
     1081 ttcgggcgct tgccgctggt cagcccgtat ttgccgtcgc cgataaaatt ttcggaaaaa
     1141 ttcaagccga aacctaaacc cgcgccgccc ataatcgagt cgcgctcgcc cgcgttctgc
     1201 cgcagataat caatcgtcgg ctgatacaaa ttggcgtaag tatttttcct gatgcgcagc
     1261 gtcattccgc ccatctgcag ggcgacgaac aaaaccagcg ccgcggcgag aagcggtttc
     1321 ggcagggatt ttttctgcca gagctcgttc agcgtgattg ccagaaacgc gacgtaaaac
     1381 gggacgatat gaaccagata cggcgtttct ttctgcccgt cgagaatcgc cataatgacg
     1441 aaataaatcg cccacatcac cagcagcgca aaatatttcc ggcgcaggct ctgcgtgaaa
     1501 ataactccca gcagcccggc gacgtagccg atcagaatca ggcttttcag gcgcacgaac
     1561 tcgccgtgac cggaagacgt atagcccaaa ccgtaggcgt gcgggtagcg gtcggtaaat
     1621 tcgcgccaga tagcctggac gggcgacgaa aaaccgctcg ttctgccgct catcaaagca
     1681 ttatcgataa actggtcgcg aaaggcttgc gggtcttgca gaacccaata gccgaagccg
     1741 gcgccgccga ttaaataggg aagcgcggcg acggcgagat gtttcaccga aagccggcga
     1801 aaatccagat aaagcgtcag gaaaatcaat gcgaaaaagg cgagaatgcc gttcgggtgc
     1861 gtcaagccgt taagcacgac caaagtttga cttaaagcga cggcaagcaa aagattacgc
     1921 tcgcgaaagt gcagataaat cgccgccgcg gcaaaaccca gcgcggcgct catcatatcc
     1981 atccgcgccg acgaagccgt atccagaata gtgtagtcac aggcgagcag ggcgagacag
     2041 ataaacgcaa tgttccggtt gccggaaagc ttcagcacga taaaatacca ggcggcaagc
     2101 agaaccaagc cccagaaaat cgagaccagg cgcatcgtga aaatgccggc gccgaacgct
     2161 ttgaacgaag ccgaaacatt aatcaggaaa agcggcatca cccagtaagt gcgctcgtcg
     2221 atgcgagtca gaggcgagtt ttccgtctcc agcacggtcg tgccgaaatg ccctttgtcg
     2281 gctagattta aagcggggct ggcaaacatt ccctcgtcta tctgcgggcg attcgtcagc
     2341 gaagacgccg ccgccagcga aagaaaaact gcggtgacca gaatgattaa aaaaaggcgt
     2401 gtgcgtgaaa tcatagaaag taattaaatc acgaatcggc gggaaatttc acaaactttt
     2461 cgggcgcgcg gacaaataaa aaagccgctc gaaggcggct ggaaaaattt agcggtcaat
     2521 cttctgcatt aactctttga atcgagcgtc ttcgcgcatc ggcgcgagca tctcgtcggt
     2581 ctcaaaccac gggcgatttt cgt
//
LOCUS       DASWNE010000277         3981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_467722, whole genome shotgun sequence.
ACCESSION   DASWNE010000277 DASWNE010000000
VERSION     DASWNE010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467722"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VGB68_05680"
     CDS             <1..232
                     /locus_tag="VGB68_05680"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY0048754.1"
                     /translation="MLTHAFETWKCIRVELKTDALNEKSRSAILRLGAKEEGIFRRHV
                     ITDSGRLRDSVYFSILDSEWQSVKERLMNFSK"
     gene            complement(289..549)
                     /locus_tag="VGB68_05685"
     CDS             complement(289..549)
                     /locus_tag="VGB68_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048755.1"
                     /translation="MLKKSRILKRLIVLSALVFCLGWFSFVDTGSRQVVLAAPCCQDC
                     PGFENPGEEVQYCSDQCGGTGNACYYDCLNWVHSCYRNCRFC"
     gene            complement(563..1078)
                     /locus_tag="VGB68_05690"
     CDS             complement(563..1078)
                     /locus_tag="VGB68_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048756.1"
                     /translation="MSKLSQKVELTANILIIVVAVLLVGVIAQKYFFGSSNDSNAPTR
                     VQPTVGTKINVPEVDLSAQPKTLVLVLQKGCRYCTESAAFYKRLQENTQNKNIKLVAV
                     LPGKIEESAAYLSELGIANMEVKQSPLNNLQTSGTPTLILTNDKGEVTNFWIGKLPAE
                     KEDEVIKQLNS"
     gene            1266..1898
                     /locus_tag="VGB68_05695"
     CDS             1266..1898
                     /locus_tag="VGB68_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048757.1"
                     /translation="MSGILKIAIIAAILTLLGGCSETSVDKNINASTANGNSSNTANG
                     ESVSNTAMIPYPGRGNTSGAPPADGDVQVVNIDPKQLKPTNPAIPAADNSEVVTALNE
                     KGAVETRTFKSHPVLARVEKTTFGREVQLKVHLKNGKVIPVQSEKIKNFTVDSAEQIL
                     QAAGIVSPKPAQNADTGAVTGTKTEVIKESKPNAAGQTPNAPPIKAPTKP"
     gene            1953..2240
                     /locus_tag="VGB68_05700"
     CDS             1953..2240
                     /locus_tag="VGB68_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048758.1"
                     /translation="MEKFTVICPCCETTLTIDAQTGAILAHEEKAKKLGSFEDLKSEL
                     DKQKETREQLFAQEMSSMKDRERLLEEKFKEAMKRAGDETDKPFRNPLDLD"
     gene            2322..3536
                     /locus_tag="VGB68_05705"
     CDS             2322..3536
                     /locus_tag="VGB68_05705"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0048759.1"
                     /translation="MEKISHYCQKCRAANQPGDVVCHRCGTRLMLVVIPPSVRHEESV
                     VPSYYEDHLLERVSLLELRLGQIAERLSMALDLMLRQAKTTQVDHFLLETLIESLNTL
                     GISEKEALTQKLRARMEGEDIRETPEKRRDQIFKNILLNHDQPNADLFGHLVKEGIRF
                     LDEGEEKQGFRTLERAAQLSARNVPLLIFLAESLFRTDKFEAAKKYLEKGCEVAPQET
                     KILLLLGVIYADEGDSENARRLLSVISTKPETSFCVNYIWGFLAAAEEKWMEALAAFN
                     EALKLRDLPEMHYLAACAYFQLERYKTAHKHLQKAVEQDENFSDAWFMQSVIFALSGE
                     QAKETEAFERAWQTKESGAECLAFFKRKKGEYPPVALPFSRLQTSEKHLLTNGARRLT
                     KLFREEIYKIIG"
     gene            3596..>3981
                     /gene="pyrF"
                     /locus_tag="VGB68_05710"
     CDS             3596..>3981
                     /gene="pyrF"
                     /locus_tag="VGB68_05710"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEY0048760.1"
                     /translation="MKSNKEKIIVALDVETAAAAREIIDELRAEVGAFKIGLQLFTAA
                     GASFVREVVAGDVRIFLDLKFHDIPNTVAKASIEAARLGVWMFNIHALGGSEMLARTV
                     AEVREACERESLRQPKIIGVTVLTSA"
BASE COUNT         1108 a          953 c         1001 g          919 t
ORIGIN      
        1 gatgctgacg cacgcttttg agacgtggaa atgtattcgc gtggaactga aaacggacgc
       61 gctcaacgaa aaatcacgca gcgcaatcct gcggctcggc gccaaggaag aaggaatttt
      121 tcgccgccac gtaattaccg attcgggaag gctgcgagat tcggtctatt tcagcattct
      181 cgattcggaa tggcagagcg tgaaagaaag attgatgaat ttttccaaat aaaaagcccc
      241 gccagagcag ggctttccta aaataaaaat tgccgaagtt gacgcgcttc agcaaaatct
      301 gcaatttcgg taacaactgt gaacccaatt caaacaatcg taataacagg cattgcccgt
      361 accgccgcac tggtcgctgc aatattgaac ttcctcgccc ggattttcaa aacccggaca
      421 atcctgacag cagggtgctg ccaaaaccac ttgccggctt cccgtatcga cgaatgaaaa
      481 ccatcccaga caaaacacaa gcgccgacaa aacgattaaa cgtttcagta ttctcgattt
      541 tttaagcatt ttttccctcc tcttaagagt ttagttgttt gataacttcg tcttcttttt
      601 cagcgggcag ctttccaatc cagaaatttg tcacctcacc tttgtcgttg gttaaaatta
      661 atgtgggcgt tccgctggtt tgaagattat tcagaggcga ctgtttcacc tccatattgg
      721 caatgcccag ttcgctgaga taagccgcgc tttcctcaat ctttccgggt aaaaccgcaa
      781 ccagtttgat gtttttattt tgtgtgtttt cctgtagccg cttgtagaaa gccgcgcttt
      841 ccgtgcagta gcggcaacct ttctgcagaa ccaaaaccaa agttttcggt tgagccgata
      901 aatcgacttc gggtacattt attttcgtcc cgacggtcgg ctggacgcgc gtcggcgcat
      961 tggagtcgtt cgaagagccg aagaaatatt tttgagctat tacgccgacc agcaaaacgg
     1021 cgacgacgat aattagaatg ttggcggtta actcaacttt ttgactaagt ttactcatat
     1081 tggtggacga aaggcttcaa aagttttaaa taaaaattac gcctgtgcgc tctaaaagtc
     1141 aagatttttt gtaaaaattt ttccttatta tttatgatta aaaaatttac ttcgactctg
     1201 ttaaacttaa gccattgttt ttagttaaat ttacaaaaat cagaaggaaa actcaggaga
     1261 aaataatgag cggcatctta aaaatcgcaa ttatcgcggc tattttaact ttactcggcg
     1321 gctgttccga aacttccgtc gacaagaata taaacgcttc gacggcgaac ggcaattcgt
     1381 cgaacacggc taatggcgaa tccgtttcaa atacggctat gattccgtat ccgggtcgcg
     1441 gaaatacgag cggcgcgccg cccgccgacg gcgatgtgca ggtcgtcaat atcgacccga
     1501 aacaattaaa gccgaccaat ccggcgattc cggcggcgga taattcggaa gtcgtaaccg
     1561 ccttgaacga aaaaggcgcg gtcgaaacca gaacttttaa aagtcatccg gttttagcca
     1621 gggtcgagaa aacgaccttc ggcagagaag ttcaattgaa agtccatctg aaaaacggaa
     1681 aagtcattcc ggtgcaatcg gaaaaaatca aaaactttac cgttgattcg gcggagcaaa
     1741 ttctgcaagc cgccggaatc gtatcgccga aaccggcgca aaatgccgat accggagccg
     1801 tgaccggaac gaaaacggaa gttataaagg aaagcaagcc gaacgctgcc ggtcaaactc
     1861 cgaatgcgcc gccgattaaa gcgccgacga aaccctaaaa ccggagaaag cctttcaaac
     1921 aaatgcgtaa actgggttat aatcggtttg ctatggaaaa gtttacggtg atttgcccgt
     1981 gttgcgaaac gactctgacg attgacgcac agaccggcgc gattctggcg cacgaggaaa
     2041 aagcgaaaaa actcggttcg tttgaggatt taaaaagcga gctcgacaag cagaaggaaa
     2101 cgcgcgaaca gctttttgcg caggaaatgt cttcgatgaa agaccgcgag cggctgctcg
     2161 aagaaaaatt caaggaagcg atgaaacgcg ccggagacga aaccgacaaa ccttttcgaa
     2221 atccgcttga tttggactga aagtgctgac tcgtgtctgc cggagcgcac gattttaata
     2281 aattactccg cgcccggcac tcacgagtca gcactcattt tatggaaaaa atctctcatt
     2341 actgccagaa atgccgcgcg gcgaaccagc ccggcgacgt ggtttgccac cggtgcggga
     2401 cgcgcctgat gctggtcgtc attccgccct cggtgcggca cgaggaaagc gtcgttccgt
     2461 cttattacga agaccatctg ctggagcgcg tctctctgct cgagctgcgt ctgggacaaa
     2521 tcgccgagcg gctctcgatg gcgctggact taatgctcag gcaggcaaag acaacgcagg
     2581 tcgaccactt tttgctggag actttgatcg aatcgctcaa cacgctcgga atctcggaaa
     2641 aagaagcgct gacgcaaaag ctgcgcgcgc gtatggaagg cgaagacata agggaaacgc
     2701 cggaaaaacg ccgcgaccag attttcaaaa atattctgct caaccacgac cagccgaacg
     2761 ccgacctttt cgggcatctc gtcaaggaag gaatcagatt tttagacgaa ggcgaagaaa
     2821 aacagggttt tcggacgctg gagcgagccg ctcagctttc cgcgcgaaac gttccgttat
     2881 taatttttct cgccgaaagc ctgtttcgca ccgataaatt cgaggcggcg aaaaaatatc
     2941 tggaaaaagg ctgcgaggtt gcgccgcagg aaacgaaaat tttactgttg ttaggcgtaa
     3001 tttacgccga cgaaggcgat tccgaaaacg cgcggcggct tctgagcgta atatcgacca
     3061 agccggaaac gtctttttgc gtaaattata tatggggatt tctggcggcg gcggaagaaa
     3121 aatggatgga agctttggcg gctttcaacg aagctctaaa gcttcgcgat ttgccggaaa
     3181 tgcattatct ggcggcttgc gcgtattttc agctcgaacg ctacaaaacg gcgcacaaac
     3241 acctgcaaaa agccgtcgag caagacgaaa atttctccga cgcctggttt atgcagagcg
     3301 tgattttcgc tctttcgggc gagcaagcaa aagaaacgga agcgtttgag cgtgcctggc
     3361 agacaaaaga atcgggcgcg gaatgcctcg ccttttttaa acgaaaaaaa ggcgaatatc
     3421 cgccagtggc tttgccgttc tcgcgtttgc aaacgtctga aaaacatttg ctgaccaacg
     3481 gcgcgcggcg tttaacaaaa ctgtttcggg aagaaattta taaaataatc ggttagattt
     3541 tgccgtcggc acaaaatgac aaaactcctt tttcgccttt ccttctattc aactgatgaa
     3601 atcgaataaa gaaaaaataa tcgtcgcgct ggacgtggaa acagccgccg cggcgcgtga
     3661 aattatcgat gagctgcgcg cggaggtcgg cgcgtttaaa atcggattgc aactatttac
     3721 ggcggcgggc gcatcattcg ttcgtgaagt cgtcgcgggc gacgtcagga tttttctcga
     3781 tttaaagttt cacgacattc cgaacacggt cgccaaagca tcgatcgaag cggcgcgtct
     3841 cggcgtctgg atgtttaata ttcacgcgct cggcggaagc gaaatgctgg cgcgcaccgt
     3901 cgcggaggtc agggaagcct gcgaaaggga aagcttgcgg cagccgaaaa tcatcggagt
     3961 tacggttttg accagcgccg a
//
LOCUS       DASWNE010000278         2203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_291534, whole genome shotgun sequence.
ACCESSION   DASWNE010000278 DASWNE010000000
VERSION     DASWNE010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291534"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2203)
                     /locus_tag="VGB68_05715"
     CDS             complement(<1..>2203)
                     /locus_tag="VGB68_05715"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEY0048761.1"
                     /translation="LGLKRVKESNGFAVVQDPDDAAYADMPLSAIATNLVDWILPVRR
                     IPESLINFRDSSERFHMTSEDDGKVVREINADESLREILTLLRVRTGHDFSHYKTPTL
                     IRRIARQLQIHNLEDIPSYLTYLRENTGEVQSLLKNLLINVTNFFRDKDAFSVLEHDI
                     VPTLFAGKKAKDTVRVWSAGCASGEEAFSMAMLLTEYADRLNDPPKIQVFGTDVDEEA
                     ITEAREHRYPESIEADVSPERLKRFFVKEGSFYRIRKELREMILFAPHNVLRDPPFSR
                     LDLIACRNLLIYLNRETQERLLEIFHFALVPSGFLFLGNSETADGIPNYYSVVDKKQR
                     IYRRRHAPLGQLLPPQMPLAGKWEVSPPLRKTAIAGRRRFSFGEMHYKLLETLAPPSV
                     LVNRDFEVQYMSESAGRFLQFKGGEPTKNLLKLVNPELLPDLRAALFSVQRDEKISRF
                     DNIHIVLDGAEALVNVTARTVDIGDEASDYILVTFEQPKSLSVAPVDGDGDGNHGRVF
                     EPDDRMESFVQRLEEDLRRTKTQLRATIEQHEVSIEELKASNEELQAINEELRSATEE
                     LETSKEELQSVNEELTTVNTELKDKIDESTRTYSDLQNLMASTDIATIFLDRQLKIKR
                     YTPPILDIFNVTPLDIGRPLEHFTNILEYDNLTADAEQVLRKLKTIEREIRDKQNRTF
                     LARLLPYRTADDRIEGVVLNFLDITERKRIERALNQSEARLRTMLESAKDYA"
BASE COUNT          447 a          607 c          629 g          520 t
ORIGIN      
        1 gcgtaatcct tcgcgctttc gagcatcgtc cgcaggcggg cttcggattg attgagcgcg
       61 cgctcgatgc gtttgcgctc ggtgatgtcg agaaaattca aaacgacgcc ctcgatgcgg
      121 tcgtccgccg tccggtacgg caaaaggcgc gccagaaacg tgcggttctg cttgtcgcgg
      181 atttcgcgtt caattgtttt cagcttgcgc agaacctgct cggcgtctgc cgtcagattg
      241 tcgtattcga gaatgttcgt aaaatgttcg agcggtcgcc cgatatccag cggcgtaacg
      301 ttgaaaatat cgagaatcgg cggcgtgtag cgtttgattt taagctgccg gtcgagaaaa
      361 atcgtggcga tgtcggtcga agccatcaga ttctgcaaat ccgaataggt gcgcgtcgat
      421 tcgtcgattt tatccttcag ctccgtgttg acggtcgtca gctcctcgtt gaccgattgc
      481 agctcttcct tgctggtttc gagctcttcg gtcgccgagc gcagctcctc gttgattgcc
      541 tgcaattcct cgttcgacgc cttgagttct tcaatcgaga cttcgtgctg ctcaatcgtc
      601 gcgcgcagct gcgttttcgt gcggcgcaaa tcttcttcca gccgctggac gaacgattcc
      661 atccggtcgt ccggttcgaa aacgcgcccg tgatttccgt cgccgtcgcc atcaaccggc
      721 gcgaccgaaa gcgatttggg ttgctcgaag gtgacgagaa tgtaatcgct cgcctcgtcg
      781 ccgatgtcaa ccgtgcgggc ggtcacgttg acgagcgctt ccgcgccgtc cagaacgatg
      841 tgaatgttat cgaaccgcga aattttctcg tcgcgctgga cgctgaaaag cgcggcgcgc
      901 aaatcgggca gcaattcggg attgaccagc ttgagcagat ttttcgtcgg ctcgccgccc
      961 ttgaattgca gaaagcgccc ggcggattcg ctcatatact ggacttcgaa atcgcgattg
     1021 accagaacgg acggcggcgc gagcgtttcg agcaatttgt aatgcatctc gccgaacgaa
     1081 aatcttcgcc tgccggcgat agccgttttg cggagcggcg gcgacacttc ccattttccg
     1141 gcaagcggca tctgcggcgg caacagctga ccgagcggtg cgtggcgtcg gcggtaaatg
     1201 cgctgttttt tatcgacgac cgagtaataa ttcggaatgc cgtcggcggt ttccgagttg
     1261 ccgagaaaaa gaaaaccgct cgggacgagc gcgaaatgaa aaatctccag caggcgttcc
     1321 tgcgtttcgc ggttcagata aatcaaaagg ttgcggcacg cgattaaatc tagccgcgaa
     1381 aacggcgggt cgcgcagaac gttgtgcggc gcgaacaaaa tcatctcgcg cagctctttt
     1441 ctgatgcggt aaaaactgcc ttccttgacg aaaaatcttt ttaagcgttc gggcgaaacg
     1501 tccgcctcga tggattccgg ataacgatgc tcgcgcgctt ccgtaatcgc ttcctcgtcg
     1561 acgtcggttc cgaaaacctg aatcttcggc gggtcgttta aacggtcggc gtattcggtc
     1621 agcagcatcg ccatcgaaaa ggcttcctcg cccgaagcgc agccggcaga ccagacgcgc
     1681 accgtgtcct tcgccttttt tccggcgaaa agcgtcggca caatgtcgtg ctccagcacg
     1741 ctgaaagcgt ctttatcgcg gaaaaaattg gtgacgttga taaggagatt tttcagcagc
     1801 gattgaacct cgcccgtatt ttcgcgcaga taagtcagat acgaaggaat gtcttcgaga
     1861 ttgtgaatct gcaactgccg cgcgatgcgc cgaatcagcg tcggcgtttt gtaatgcgaa
     1921 aaatcgtgtc ccgtgcggac tcttaaaagc gtcagaatct cgcgcagcga ttcgtcggcg
     1981 ttgatttccc tgaccacctt gccgtcgtct tcgctcgtca tatgaaaacg ctctgagctg
     2041 tcgcggaaat ttatcaggct ttccggtatc cgccggacgg gcaaaatcca gtcgacgaga
     2101 ttcgtcgcaa tcgcgctgag cggcatatcg gcgtaggcgg cgtcatccgg gtcttggacg
     2161 acggcgaaac cgttcgattc cttgacgcgc ttgagtccga gcg
//
LOCUS       DASWNE010000279         3299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_644089, whole genome shotgun sequence.
ACCESSION   DASWNE010000279 DASWNE010000000
VERSION     DASWNE010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644089"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..2055
                     /locus_tag="VGB68_05720"
     CDS             226..2055
                     /locus_tag="VGB68_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0048762.1"
                     /translation="MAEEKKKKVNYTSAWREARDLIWKARWRLAFGSSLMLVSRLAGM
                     VLPASTKFIGDEVFVNRRYELLKWIALAIGISSLIQAVTSFALSQILGVAAQRAITDM
                     RKRVQQKIERLPISYFDSTQSGQLISRIMNDAEGIRNLVGTGLGQILGSIVTASIAVC
                     VLFYINWRLTAATIVVLVAFGGVLAYAFKKLRPIFRERNVINAEVTGRLGESLGGIRI
                     VKAYTAEKREELSFARGAHRLFRNIAKSLTGVSAVGAFSSIVIGAISVVMILIGGSAV
                     QSGAMTLGDFLMYISFTFLLAMPIVELTSIGTQITEAFAGLDRIREVMQMTTEDEEDT
                     DKQPLPQVAGTIDFENVYFEYEENVPVLKGVSFHSEAGKTTALVGSSGSGKSTILSLV
                     LNFIQPNKGVIKIDGKDLQAVKLRDYRSHLGVVLQDNFLFDGTILDNIRFSNPEANLR
                     EIKEVCRIANADEFIEKFPEAYNTIVGERGVKLSGGQRQRIAIARALLANPRILILDE
                     ATSSLDSESEALIQEGLRRLREGRTTFVIAHRLSTIRSADQILVVETGEIVERGTHEE
                     LIALGGRYKQLYDKQYRFEQNLFVNPGEDFTTGQAETLTQTKL"
     gene            complement(2082..3146)
                     /locus_tag="VGB68_05725"
     CDS             complement(2082..3146)
                     /locus_tag="VGB68_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0048763.1"
                     /translation="MRITSGFFKITAAIAVLTILVTNAAAFATAKTVDGAGGGAPPAR
                     PCFDFYGSGRTTFATFQRLSGGNIIWRLLNNGGAGADVVPYGLSATDAVTPGYFDGDN
                     KADFNVWRRGTAANPAATYLLSPTTAPNTVVGIQWGRHDGTASASNDSAGFEGDYDGD
                     GRTDPTVVRTIGGTYNWYILRSGSSTFSAVAFGNSATDIRLSGADYTGDGKDEIAVYR
                     TGAGADTFLIGDTNTGALVRAQRWGEFDTDNVVIGDFVGDRRADFVVWRSFAAGANNG
                     FWHILENGGASQFSYKFGIGNSDLAVCGDYTGDGKSDMAVWRPSNQTFYWASVSGTPT
                     QGQQQFGAAGDTPMGALKVY"
BASE COUNT          747 a          883 c          914 g          755 t
ORIGIN      
        1 tttattttaa caagtatttt atcaaatgtt cgccggtcgg ctcgaccttt cttttgataa
       61 tgttcaaagt gttgctcgga accaatcgaa aagcgccccg gtcggagatt cccggcacca
      121 caatcgacag attaaataaa acaatctgaa ttctaatgga aaagcccgca actgcaaaac
      181 gctccaattt gactttttta ccggcggcgc gcaaatttta aataaatggc ggaagaaaag
      241 aaaaagaaag taaactacac gagcgcgtgg cgcgaagcca gagatttgat ttggaaagcg
      301 cgctggcgtc tggctttcgg aagctctctg atgctcgtca gccgtctcgc cggaatggtt
      361 ctgccggctt cgaccaagtt tatcggcgac gaggttttcg tcaatcgccg ttacgagctg
      421 ttgaaatgga ttgcgctcgc catcggcatc tcgtcgctga ttcaggcggt cacgtctttc
      481 gcgctgtcgc agattctcgg cgtggcggcg cagcgcgcga ttaccgatat gcgcaagcgc
      541 gtccagcaga aaatcgagcg gctgccgatt tcgtatttcg attcgacgca gagcgggcaa
      601 ttgatttcgc gcattatgaa cgacgccgaa ggcattcgca atctggtcgg aaccgggctc
      661 ggacaaattc tcggaagcat cgtcacggcg tccatcgccg tctgcgtttt gttttatatc
      721 aactggcgct tgacggcggc gacgattgtc gtgctcgtcg ctttcggcgg cgtgctggct
      781 tacgcgttca aaaaactgcg cccgattttt cgcgagcgaa acgtgataaa cgccgaagtg
      841 acgggacgcc tcggcgaatc gctcggcggc atccgcatcg tcaaggctta cacggcggaa
      901 aaacgcgaag agctttcttt cgcgcgcggc gcgcacaggc tttttagaaa tatcgccaaa
      961 tcgctgaccg gagtttcggc ggtcggcgcg ttttcgtcaa tcgtcatcgg cgccatcagc
     1021 gtcgtgatga ttctgatcgg cggcagcgcc gtgcaatcgg gcgcgatgac gctcggcgat
     1081 tttctgatgt atatctcttt cacttttctg ctggcgatgc cgattgtcga gctgacttcg
     1141 atcgggacgc agataacgga agccttcgcc ggtctcgacc gcatccgcga ggtgatgcag
     1201 atgacgaccg aagacgagga agacaccgat aaacagcctc tgccgcaggt cgccggaacg
     1261 attgattttg aaaacgtcta ttttgaatac gaagaaaacg tgccggtttt aaaaggcgtt
     1321 tcgtttcatt cggaagccgg aaagacgacg gcgctggtcg gctccagcgg ttcgggcaaa
     1381 tcgacgattc tgagcctcgt ccttaacttt atccagccga acaaaggcgt tataaaaatc
     1441 gacggcaagg atttgcaagc cgtcaaactg cgcgattacc gctcgcatct gggcgtcgtt
     1501 ttgcaggata attttttgtt tgacggcacg attctggaca atattcgctt ctcgaacccg
     1561 gaagcgaatc tgcgggaaat caaggaagtc tgccgcatcg ccaacgccga cgagtttatc
     1621 gaaaaatttc ccgaagcgta taacacgatt gtcggcgagc gcggcgtcaa gctttccggc
     1681 ggtcagcgcc agcgcatcgc catcgcccgc gcgctgcttg caaatccgcg aattttgatt
     1741 ctcgacgaag cgacttcttc gctcgattcg gaaagcgaag cgttgattca ggaaggcttg
     1801 cgccgcctgc gcgagggcag gacgactttc gttatcgcgc accggctttc gaccattcgc
     1861 tccgccgacc agattttagt cgtcgaaacg ggcgaaatcg tcgagcgcgg aacgcacgaa
     1921 gaactgattg cgctcggcgg gcgctacaaa cagctttacg ataaacaata ccgtttcgag
     1981 cagaatcttt tcgtcaatcc gggcgaagat tttacgaccg ggcaagccga aactctgacg
     2041 cagacgaaat tataaaatat gcgcgaacca cgaatgaaag tttaataaac tttcagcgcg
     2101 cccatcggcg tgtctccggc tgcgccgaac tgctgttgac cttgcgtcgg cgtgccggag
     2161 acggacgccc agtagaaagt ttgatttgaa ggtcgccaga cagccatatc cgatttgccg
     2221 tcgcccgtgt aatcgccgca gacggcaagg tcggagttgc cgattccgaa tttgtaggaa
     2281 aattgcgacg cgccgccatt ttccagaatg tgccagaaac cgttgttcgc gccggcggcg
     2341 aaactgcgcc agacgacaaa atcggcgcgg cggtcgccga caaaatcgcc gatgacgaca
     2401 ttatcggtat cgaattcgcc ccagcgctgc gccctgacga gcgcgcccgt gttggtgtcg
     2461 ccgatcagga acgtatcggc tcccgcgccg gtgcgataga cggcgatttc gtctttgccg
     2521 tcgcccgtgt aatcggctcc gctcagcctg atgtcggttg ccgaattccc gaaggcgacg
     2581 gcgctgaaag tgctgctgcc gctgcgaaga atataccagt tataagttcc gccgattgtg
     2641 cgaacgaccg tcgggtcggt tctgccgtcg ccgtcgtaat cgccttcaaa accggcgctg
     2701 tcgtttgagg cggaagcggt gccgtcatgt ctgccccact gaatgccgac cacggtattc
     2761 ggggcggtgg tcggactcag taaataagtt gccgccggat ttgccgccgt gccgcgccgc
     2821 caaacgttaa aatccgcttt attgtcgccg tcgaaatagc cgggcgtgac agcatcggtc
     2881 gccgacaaac cgtaaggaac gacgtccgcg cccgcgccgc cattgttgag aagccgccaa
     2941 attatattgc ctcccgataa tctttgaaaa gtggcgaatg tcgtgcgccc cgaaccgtaa
     3001 aagtcaaagc agggtctggc gggcggagcg ccgccgcccg cgccgtcaac tgtttttgcg
     3061 gtcgcaaaag ccgccgcgtt tgttactaag attgtcaaaa cggcaatcgc tgcggtgatt
     3121 ttgaagaagc cggaagttat ccgcatagtt tcctccgtgt taatcgttat ttttctttct
     3181 ggaagtatat gcgtgagaac ttataaaagc cagccatttt ttttaggaga tttatttcga
     3241 cgaaaatcaa atttatgtaa tcatttataa atccgccgaa aaagtatttg tgagcgaga
//
LOCUS       DASWNE010000280         4337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_220572, whole genome shotgun sequence.
ACCESSION   DASWNE010000280 DASWNE010000000
VERSION     DASWNE010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220572"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..83)
                     /gene="ffs"
                     /locus_tag="VGB68_05730"
     ncRNA           complement(<1..83)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VGB68_05730"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(182..269)
                     /locus_tag="VGB68_05735"
     tRNA            complement(182..269)
                     /locus_tag="VGB68_05735"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(233..235),aa:Ser,seq:gga)
     gene            complement(425..661)
                     /locus_tag="VGB68_05740"
     CDS             complement(425..661)
                     /locus_tag="VGB68_05740"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEY0048764.1"
                     /translation="MENVELKNRTKDFAIRIVKLFAALPKITEAQILGKQLLRSGTSV
                     GAQYREAQHAKSDADFTSKIEGSLQELEETAYWL"
     gene            complement(702..791)
                     /locus_tag="VGB68_05745"
     tRNA            complement(702..791)
                     /locus_tag="VGB68_05745"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(755..757),aa:Ser,seq:cga)
     gene            complement(858..1346)
                     /gene="tadA"
                     /locus_tag="VGB68_05750"
     CDS             complement(858..1346)
                     /gene="tadA"
                     /locus_tag="VGB68_05750"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF008113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEY0048765.1"
                     /translation="MSLEIDQFWMREAIRAAQEAGQRDEVPIGACLIDKKGILLASAG
                     NKTRLLCDPTAHAEILVLREAAAKIGNYRLTETTLYTTIEPCAMCAGALVQARVERLV
                     YAALDERFGAVETLFRICDSSSLNHQMEITSGVLATECRKLVQDFFKRRRAKGERRKA
                     KE"
     gene            complement(1532..2332)
                     /locus_tag="VGB68_05755"
     CDS             complement(1532..2332)
                     /locus_tag="VGB68_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048766.1"
                     /translation="MTKRFEILILVFLTLILMTGNAFAQSAKWQQVGKSGDWRNTKLM
                     AGMDGAIWTIESSGSLYRTEKNGVWKQIGEAGAWKNTRLMTVMDGELWTVESNGSLYR
                     TDKNGNWKQVGKGGDWANTRALVALDGELWSVEQNGDLYRTDKNAAYRKVGDAGILSD
                     VRHLVVLDGSLWSIEKDGSLYRIEKNGKWQRVGKAGEWRETRHAVTMNGYIWSIESNG
                     NLYKTDKTGRWVAIDGNGAYKNTVTLIGLDGELWSIESDGTLYKTTVH"
     gene            2591..3517
                     /locus_tag="VGB68_05760"
     CDS             2591..3517
                     /locus_tag="VGB68_05760"
                     /inference="COORDINATES: protein motif:HMM:NF021539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2270 domain-containing protein"
                     /protein_id="HEY0048767.1"
                     /translation="MRDEVRTAGYAADEEEIHISKASPLVRSLAEEVRARSEKPKNNP
                     LAIPQKLSPSEFNTAMVHFYRGEIQRSNVWRNRLDATTNWAVITTGATLSFVFSSPDN
                     PPFVIPINSILVAIFMFMEARRYRYYEVWASRVRVLETGYFAPMLSHRTIAPDQEWAD
                     HISADLISPHFNISEWEAVGRRLRSNYRWIFILLALSWSLKVYIHPYPAPNLEVFTQR
                     AQVGLVPGSLVIAIGFVFNALMFLIAIGTLRLKDASSEVLPLDKFEWHPLKQVSDWAE
                     SSLKRRNTIRRAKKARHRVRSVQTDTSKIHNS"
     gene            3680..3973
                     /locus_tag="VGB68_05765"
     CDS             3680..3973
                     /locus_tag="VGB68_05765"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEY0048768.1"
                     /translation="MSSEVSLEDRAHALENEYFHRKERELIEKMRSKLQSEATEASTL
                     QCPKCDGKLVESNFENIKVDVCNSCHGVWLDAGELAQIAHHEENKGSWFGRWF"
     gene            4090..>4337
                     /locus_tag="VGB68_05770"
     CDS             4090..>4337
                     /locus_tag="VGB68_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048769.1"
                     /translation="MNKRRIFNQSLWLVLALVLPFSVRAQQQQQTAPQTEKTISRPAA
                     QVQTLRLDSRLTAREMPYRVILPNDYEAKEKAVERYPVV"
BASE COUNT         1043 a         1101 c         1021 g         1172 t
ORIGIN      
        1 cgcaacacac gctgaaattg ctgccgttgc tctgttccca gcctagcgga gttcacaact
       61 caaacgttgt gcggagcctg aagccataac ttaaaggata aaggctgagg gcgaaaggat
      121 aaagtaaaaa caattcctta gtttttcgcc tctcgcctgg agcctttatc ctttaaagaa
      181 gcggaaaggg tgggattcga acccacggaa cccttttgag gttcacagca tttccaatgc
      241 tgccaattca accactcttg cacctttccg aaagggtaaa ggcgaaggga taaaggcgaa
      301 agatttatcc cgattttgcc ttaacttttt tagcaatcgt tataaagatt gctattagtt
      361 cctttgtttc ttgttttatt ggctgtagtt tttgagaact gaaaaattcc agttcttcga
      421 ttaattacaa ccaataagcg gtttcttcta attcttgcaa agagccttcg attttactgg
      481 taaaatcggc atcagattta gcgtgctgcg cttcacgata ttgcgcgcct actgacgttc
      541 ccgaacgcaa aagctgtttt cctaaaatct gcgcttctgt aattttaggc aaagcggcaa
      601 aaagtttaac aatcctaatg gcaaaatctt tagtcctgtt ttttaattca acattttcca
      661 aatctttcgc ctttcgccgt tagccttcat cctttataaa atggcggaga gggtgggatt
      721 cgaacccacg gtacccgtta gggtacaaca attttcgaga ctgtccaatt caaccactct
      781 tgcacctctc cgtttttgtt aaagtataaa gggtcaggga taaagggtaa agccgaagga
      841 aataagaata taaaaaatta ttccttagct tttcgccttt cgcctttcgc ccttcgcctt
      901 ttaaagaaat cctgcactaa tttcctgcat tccgtcgcca gaacgcccga cgtaatttcc
      961 atctgatgat tcaaagaact gctgtcgcaa attctgaaaa gcgtttcgac cgccccgaag
     1021 cgttcgtcca aagcggcgta aaccaatctt tcaacgcgcg cctgcacgag cgcgcccgcg
     1081 cacatcgcgc aaggttctat cgtcgtataa agcgtcgttt cagtcaaacg atagttgccg
     1141 attttcgccg ccgcttcgcg caaaaccaga atctcggcgt gcgccgtcgg gtcgcagagc
     1201 aggcgtgttt tgttgcccgc cgaggcgagc agaatgcctt ttttatcaat caaacacgcg
     1261 cctatcggaa cttcgtctct ttgcccggct tcctgcgcgg cgcgtatcgc ttcgcgcatc
     1321 cagaactggt caatctccaa agacataaaa cgattgtagc cgacggcgcg ggcgaatttg
     1381 aaatgataag tttctcaaat tacaaagtca agaagccaat gtgaatgcct tattccattc
     1441 ggattgagcg gctcgcaaaa tcaacttcgg caaacgaagg attaggaaac gattccggct
     1501 ggcatcttta caaaatttcg aaattaaatg cttaatgaac cgttgttttg taaagcgtcc
     1561 cgtcactttc gatgctccat aattcgccgt ccaatccgat caaagttacc gtgtttttgt
     1621 aagcgccgtt gccgtcaatc gcgacccatc tgccggtttt gtcggtttta tacagattcc
     1681 cgttgctttc gatgctccag atatagccgt tcatcgtcac ggcgtggcgg gtttcccgcc
     1741 attcgcccgc cttcccgacg cgctgccatt ttccgttttt ttctatccgg taaagactgc
     1801 cgtccttttc gatgctccat aagcttccgt ctaaaacgac gaggtgacga acatcggaaa
     1861 gaattccggc gtcgccgact tttctgtaag ctgcgttttt gtcggtgcgg tataaatcgc
     1921 cgttttgctc gacgctccac aattctccgt ccagagcgac taatgccctg gtattcgccc
     1981 aatcgccgcc tttgccgacc tgcttccaat tgccgttttt atctgttcga tagaggctgc
     2041 cgttgctttc gacggtccat aattcgccgt ccataacggt catcaggcgc gtgtttttcc
     2101 aggctccggc ttcgccgatt tgtttccaga cgccgttctt ttccgtgcga tacaacgagc
     2161 cggagctttc gattgtccaa atagcgccgt ccattccagc cataagtttc gtgttgcgcc
     2221 aatcgccgga tttgccgacc tgctgccatt tggcggattg cgcgaaagca tttccggtca
     2281 tcaatatcaa agttagaaaa accagtatca gaatctcaaa tctttttgtc attttttctc
     2341 cgaaagttaa ttaatactcg attcagggcg atataaagca tttaatcgac agggtgtttt
     2401 gaaagcggtt cggatgttag caaagccgtt cggaaaagtc aaaaatttca atgctccgga
     2461 aatgatatgt attcgcgtct cgcatttctc tattcgattt gcctttaaaa tttcttgcat
     2521 cgagccggta aagtgttaaa cttaaattga gtcgattttc tgcgctcctc cggctcgcaa
     2581 caaatgcctt atgagagacg aagttcggac ggcaggttat gccgccgacg aagaagaaat
     2641 tcacatcagc aaagccagtc cgcttgtcag gtcactcgcc gaagaagttc gcgcccgcag
     2701 cgaaaagccg aaaaataatc cgctcgccat tccgcagaag ctttcgcctt cggaatttaa
     2761 cacggcgatg gtgcattttt atcgcggcga gattcagcgt tcgaacgtct ggcgcaaccg
     2821 gctcgatgca acgacgaatt gggcggtcat cacgaccggc gcaacgcttt cgttcgtttt
     2881 cagctcgccc gataatccgc cgttcgtgat tcctataaat tcgattctgg tggcgatttt
     2941 tatgtttatg gaagcgcggc gctatcgtta ttacgaggtt tgggcgagcc gcgtgcgcgt
     3001 tctggagacc ggctatttcg cgccgatgct ctcgcaccgg acgattgcgc ccgaccagga
     3061 atgggctgac catatttcgg ctgacttgat ttcgccgcat tttaacatca gcgaatggga
     3121 agccgtcggc agacgcctgc gcagcaatta cagatggatt tttattctgc tcgctttgag
     3181 ctggagtctg aaagtttata ttcacccgta tccggcgccg aacctcgaag tttttacgca
     3241 gcgcgcgcag gtcgggctgg tgcccggaag cctggttatc gcaatcggtt tcgttttcaa
     3301 tgctttgatg tttctgattg cgatcggcac tctgcggctg aaagacgctt cgagcgaggt
     3361 tctgccgctc gataaatttg aatggcatcc tctaaaacaa gtgtccgatt gggcggaatc
     3421 gagcctgaaa cgccgcaaca cgattcgccg cgccaaaaaa gcccgtcacc gcgttcgctc
     3481 ggtgcagacc gatacgtcga aaattcacaa cagctagagc caaaaaagac ggaagacaga
     3541 agtcggaaga cagccgaatt tgaagtcggt tttgaggtca tagtagccga accgctttct
     3601 atcttctgac ttccgtcttc tgtcttcttc taatttgtga tatttttttc ttaaataact
     3661 ttattaagga gaaaaaatta tgtcatcgga agtaagtttg gaagaccgcg cgcacgcgct
     3721 ggaaaacgag tattttcacc gcaaggagcg ggagcttatc gaaaaaatgc gctccaagct
     3781 tcagtcggaa gcgactgaag cctcgacttt gcaatgcccg aagtgtgacg gcaagctggt
     3841 cgagtcgaat tttgagaata tcaaggtcga tgtctgcaac agttgtcacg gcgtctggct
     3901 cgacgcgggc gagttggcgc aaatcgcgca tcacgaagaa aacaaaggca gctggttcgg
     3961 tcgctggttc taaaaaagtg taagagcggg gagaaagcga gccggcgagt aaattgcatt
     4021 tgcccgggct tctccccggc tgctctattc ccaaactctc ctgctttttc gtttaaggat
     4081 taactgcaaa tgaacaaacg tcgaattttc aatcagtctc tgtggctggt gctcgccctt
     4141 gttttgcctt tttccgtccg tgcgcagcag cagcagcaga ccgcgccgca aactgaaaaa
     4201 actatttcgc gccccgctgc gcaggttcag actttgcggc tcgacagcag gttgaccgcg
     4261 cgggaaatgc cttatcgcgt aatcttgcca aacgattatg aagcgaaaga aaaagccgtc
     4321 gagcgttacc cggtcgt
//
LOCUS       DASWNE010000281         2722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_644132, whole genome shotgun sequence.
ACCESSION   DASWNE010000281 DASWNE010000000
VERSION     DASWNE010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644132"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..893)
                     /locus_tag="VGB68_05775"
     CDS             complement(48..893)
                     /locus_tag="VGB68_05775"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HEY0048770.1"
                     /translation="MTKKHGKGSLIAVGGGESSEITEDSIKIIEKFLELSGGLSKAKI
                     IVMTVATDTPEDAEKRYEEVFKQLKFKNFEFLNITDRSEAFADSILQKIESASGLYFT
                     GGNQLHVTALTGGTPLHLLILERFDKGLTIGGTSAGAMMMSSSTLLSGSGDTAPRLGA
                     IEVAPGMELLESSIIDTHFSQRGRHGRLLSSVAHNPQVLGIGIDERTAMVVQDGKFEV
                     IGEGAVTVICAKNSMHTNVPYIRTEETIGIFGVNFHVLPEGYKYDLLNREPITPNIKE
                     MLKES"
     gene            complement(1051..1230)
                     /locus_tag="VGB68_05780"
     CDS             complement(1051..1230)
                     /locus_tag="VGB68_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048771.1"
                     /translation="MTEEKKNSKKRQTKAGMSAKNNKQTATGKDKTGSEKKPENTPKM
                     SKEKAEKRGSDKPLH"
     gene            complement(1321..2118)
                     /locus_tag="VGB68_05785"
     CDS             complement(1321..2118)
                     /locus_tag="VGB68_05785"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HEY0048772.1"
                     /translation="MENEQNLKQYVETIAQWIADSEHIVAFTGAGISTDSGIPDFRGP
                     NGVWTRRDAGLTPPSWKVSPDKIMANASHLALVELQDAGKLQFLITQNTDNLHRQSGI
                     KPEILAELHGNGRLVRCLGCDRQYTRPEVGWDAARFGPGYRTQKPVAGQPVCPQCGGR
                     LISSVVNFGDPMPQKELQSASLHALSCDLMLVLGSSLVVNPAASLVAIAIENGARTVL
                     INEGETPYDEVVSLRLPVKIGEVLPAAAEKVRQLIGGGMETAGKSSS"
BASE COUNT          592 a          691 c          666 g          773 t
ORIGIN      
        1 cggtcttgcc gatgcctcag aacaggcatt tttcagcacg agaatcttta actttccttc
       61 agcatttctt taatattcgg cgtaatcggc tcgcggttca gcaaatcgta tttgtagcct
      121 tcgggcaaaa cgtgaaaatt gacgccgaaa ataccgatgg tttcttcggt tctgatataa
      181 ggaacattgg tgtgcatcga gtttttggcg cagatgaccg tgacggctcc ttcgccgatg
      241 acttcgaatt taccgtcctg cacgaccatc gcggttcgtt cgtctatgcc gatgcctaag
      301 acctgcgggt tatgggcgac cgatgaaagc aggcgtccgt gtcgcccgcg ctgggagaaa
      361 tgcgtgtcga taatcgagct ttcgagcagt tccataccgg gcgcgacctc gatggctccg
      421 agcctgggag ccgtgtcgcc cgaaccggaa agaagcgtcg aagaagacat catcatcgcg
      481 ccggcgctcg tcccgccgat agttaaacct ttgtcgaatc tttcaagaat taaaagatga
      541 agcggagttc cgccggtcag agccgtcacg tgaagctggt tgccgccggt aaagtataag
      601 ccgctggcgc tttcgatttt ttgcaagatg gaatcggcga aagcttcgct gcggtcagtg
      661 atattgagaa attcaaaatt tttgaatttc aattgcttga acacttcctc gtagcgcttt
      721 tcggcgtctt ccggcgtatc ggtggcgacc gtcataacga taattttcgc tttgctcaag
      781 ccgcccgaca attccagaaa tttttcgatg attttaatcg aatcttcagt aatttcgctc
      841 gattctccgc cgccgaccgc gattaagctg cctttgccat gttttttcgt cataagcttt
      901 tcttttattt gataattcta aacagttaac agtaacaaat ctgtctgcaa gacgacaata
      961 atgagatttc ggattttttg atatcggtat ttctcgaaaa gaaacgtttt tgagcatcgc
     1021 agcgctttcc gggtaaaaac cggtatcaaa ttaatgaagc ggtttatcgc tgccgcgctt
     1081 ttccgctttt tctttgctca ttttcggtgt attttcaggt tttttttcag aaccggtctt
     1141 atctttcccg gttgcggttt gcttattgtt ttttgccgac atcccggctt tggtttgtct
     1201 ctttttgctg ttcttttttt cttccgtcat ttcttttccc ctcaattatc tcttttgatt
     1261 tatcgaaaaa atcagcccga aatttcctgc ggctgagtta attttgccgt tatcacggat
     1321 ttacgagctg cttttaccgg cagtttccat tccgccgccg ataagctgtc tgactttttc
     1381 cgccgccgcc ggcagcactt cgccgatttt aaccggaagc cttaaactga cgacctcgtc
     1441 gtaaggcgtt tcgccctcgt taatcagcac ggtgcgcgcg ccgttctcta tcgctatggc
     1501 gaccagcgaa gccgccgggt tgacgacgag cgaagagccc aaaaccagca tcaaatcgca
     1561 gctcagagcg tgcaggctgg ctgactgcaa ttctttttga ggcatcgggt cgccgaaatt
     1621 gaccaccgac gagataagcc gcccgccgca ttgcgggcag acgggttgtc ccgccaccgg
     1681 cttctgcgtc cggtagcccg gaccgaaacg cgcggcgtcc cagccgactt ccggtctggt
     1741 gtattgacgg tcacagccca ggcagcggac gagtcgcccg ttgccgtgca gctccgccag
     1801 aatttccggc ttaatccccg attggcggtg cagattgtcg gtattctgcg taatcagaaa
     1861 ctgtagtttc ccggcgtcct gcagctcgac gagcgccaga tgagaggcat tcgccataat
     1921 cttatcgggc gaaactttcc agctcggcgg cgtcagaccg gcatcgcgcc tcgtccagac
     1981 gccgttcgga ccgcgaaaat ccgggatgcc ggaatcggtg ctgatgcccg cgccggtgaa
     2041 agcgacgata tgttcgctgt cggcaatcca ttgcgcgatt gtttccacgt attgtttgag
     2101 attttgttcg ttttccattt ttgataaatc agctctctga aaaatattcg ggcgtccgcc
     2161 ttatttctcc ggtttttgaa gacgcgtcac gcttcaaact cctgtttcac gactaaaaaa
     2221 taacggtttt acggcgctcg tcgcatcgca gcgcgtgacg gcgtcaaatt gtcctggtag
     2281 ccatctatct cgtttactcg tccgcctctt tcaaaaagcg gaggcggcgg agcgcggcta
     2341 aaaattgaag ttgataaata atcttttttc gcttaccgaa cagtttatta atttgagtat
     2401 gctaaatgga aaagacttaa tctgtattgc ttcatacatt cgaatcctat ttcctgaatt
     2461 taaaatggca ggatgccgca gccgcagccg gcgcgcttta ttttgttcgg taaaggcgct
     2521 cggcggattg gttagccgta aggaagagcg ggtcgccggt tgcgccttca acacaaaaac
     2581 gatttattgt ttccgaattt tctttcctat ttttccggca ggctctaaaa cagtagggag
     2641 tgactaaata ggcgttgata tgatattttg ggcaaatgag tcaagactgt ccaagctgtg
     2701 aagctcaaga acaacaaata aa
//
LOCUS       DASWNE010000282         5223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_256804, whole genome shotgun sequence.
ACCESSION   DASWNE010000282 DASWNE010000000
VERSION     DASWNE010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5223
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256804"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VGB68_05790"
     CDS             complement(<1..300)
                     /locus_tag="VGB68_05790"
                     /inference="COORDINATES: protein motif:HMM:NF039315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HEY0048773.1"
                     /translation="MNQTAFNKESAFPPLPLEQWRATKNTLHLYLQIVGKIRLATFPR
                     LNHWWHVPFYVSTRGLTTRPIPYGFGNFEIEFDFIDHALKILTNRGERREFALEDG"
     gene            complement(369..983)
                     /locus_tag="VGB68_05795"
     CDS             complement(369..983)
                     /locus_tag="VGB68_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048774.1"
                     /translation="MKKPIKILLQTTIPFAENDWHIDRFSLLREHLASLKDENGSPLF
                     EVAARNLELNEEGNDRLLSALDESDFDELCDSIHELLQKPDGGVIEYFPAHPHEGGVG
                     VPENEMSARVVARGKSKISGKDFNLIVAFERSEDRHGNRLGRGIAESSFHHLVDYNWD
                     VRKGCPDFVEEAPGEGYRKNPRALNDVKTYVENLAKWLAPEQQP"
     gene            complement(1091..1363)
                     /locus_tag="VGB68_05800"
     CDS             complement(1091..1363)
                     /locus_tag="VGB68_05800"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEY0048775.1"
                     /translation="MSKCEHLSEIKAVTPSADGCEDCLKTGGRWVHLRLCETCGHVGC
                     CDNSPNRHATKHFHASTHPIIKSFEPGEEWGYCYVDDMFYETLPAV"
     gene            complement(1490..1753)
                     /locus_tag="VGB68_05805"
     CDS             complement(1490..1753)
                     /locus_tag="VGB68_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048776.1"
                     /translation="MQRLRNADCRVLPVVANCYYSDKIPESFLRIKQPKILAFAGSLR
                     EKTYTKRIVKVAAEEMRALFVPLDERFAPPRKSVGNERGEAVR"
     gene            1861..2751
                     /locus_tag="VGB68_05810"
     CDS             1861..2751
                     /locus_tag="VGB68_05810"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HEY0048777.1"
                     /translation="MKCLTVLLICGCFAFSGAAQQTPVDALNRRQESKETAVTEKAGE
                     AKITEKIGKFTEFSPAAKEKFGFPLSPKEKPQPQKFDFPEPENQTEKKAQIDAQNNRN
                     RWVLPRGTIEYGAEIGYANEIATWMSGPKYYDIRGHRHLLGSFRWGRILGNKGIVTVS
                     WAVEFVPINIAIGNEVDNPRFMPGSTTETPTKRENTFGVGINPASFRFIFFPKWRLRP
                     QLGAGIGLTRHFNRVPTIDGTKGNVHIDFQIGGQYMISERRALNFGYKYYHLSNVYLA
                     QRNPGYNVNMFFVGYSIFKR"
     gene            2748..3263
                     /locus_tag="VGB68_05815"
     CDS             2748..3263
                     /locus_tag="VGB68_05815"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEY0048778.1"
                     /translation="MIKPFQNLSPRIHRTAFIAENAVIIGDVEIGENSSVWYGSILRG
                     DVNHIRIGARTNVQDACVLHVSAGTHPTVLEDEVTLGHRVTLHGCYVETGCLIGIGAI
                     VMDGARVGRNSLIGAGSLVTPNTEIPPRSLVLGSPARVKRELSDAEVKDLEKFWHNYV
                     SYSRIYKGEPV"
     gene            3306..4172
                     /locus_tag="VGB68_05820"
     CDS             3306..4172
                     /locus_tag="VGB68_05820"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HEY0048779.1"
                     /translation="MLGILRLAVGSLFLILTVLFFTVSAQQTNDSLKEDLQSETAFAE
                     STRTVIEKESFIKREARFFTPAENRLISPKSVETGADEPQASTERSVYRARRGAREYN
                     FEIGVSPFEPTNFNGRKEFNTAGRKFGTIDFRIGRVLGTSKGITYTYFFGFSPLVVAL
                     KNEVRNPAYISPAATPNVYRTRRETSYAFGFTPANFRFTFFPGSRIKPYVQGGAGVLF
                     FNKPIPIPESRRMQFMGDLGGGFIVHLKNPKQFLSFGYRYFHISNGNIGGKRWNPGYN
                     ANIFYIGFSFFK"
     gene            4274..5017
                     /locus_tag="VGB68_05825"
     CDS             4274..5017
                     /locus_tag="VGB68_05825"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HEY0048780.1"
                     /translation="MRKFFAAFACLSSLFIIIAVCPSAIFTQDLTSLQKKAVTAETGV
                     VSTPQTSGTSDFDEKNELSVWGGFAPDMPRVFGGARPSSYGQLAVRYSRRIATSDSLA
                     LKYTIDFIPLAVINYDRRPIIQTAPNTFTVQSNNTTNYAFGLTPVGLQLHFRRKAKIQ
                     PFISANAGLLIFNKTIPDDRSAVFPNLNGKHLNFTLAGGGGVDFLTDAGRTFTVGFKF
                     HHISNASTGNINPGFDQNLFYVGYTFKKW"
BASE COUNT         1294 a         1308 c         1276 g         1345 t
ORIGIN      
        1 accgtcttcc agcgcaaact cgcgccgctc gcctcgattc gtcaaaattt tcagcgcgtg
       61 gtcgataaaa tcgaattcaa tctcgaaatt gccgaaaccg tacggaatcg ggcgcgtcgt
      121 caagccgcgc gtcgaaacat agaacgggac gtgccaccag tgattgagcc gcggaaaagt
      181 ggcgagcctg attttcccga caatctgcaa ataaagatgc agagtgtttt tcgttgcccg
      241 ccactgttcg agcggcagcg gcgggaaggc gctttcttta ttaaatgcag tttgattcat
      301 aaacttgaaa cctgaaaccg gcaatctgaa acttacgcgt taaatcgccc gcttgcggcg
      361 acaggcggct acggctgctg ctccggcgcg agccatttcg ccagattttc aacgtaggtt
      421 tttacatcat ttaaggctcg cggatttttt cgataacctt cgcccggcgc ttcttcgaca
      481 aaatcgggac agccctttcg gacgtcccag ttgtaatcga ccagatgatg aaagctggat
      541 tcggcgatgc cgcgcccgag ccggttgccg tgccggtctt cgcttctttc aaaagccacg
      601 attaaattaa aatcttttcc cgaaattttg cttttgcctc gcgccacgac gcgcgccgac
      661 atttcatttt ccggaacgcc gacgccgcct tcgtgcgggt gcgcgggaaa atattcgata
      721 acgccgccgt ccggtttttg caaaagctcg tgaatcgaat cgcacaactc gtcaaaatcg
      781 gattcgtcca gagcgcttaa tagacggtcg tttccttctt cgttcagttc cagattgcgc
      841 gccgcgactt cgaaaagcgg gctgccgttt tcatctttta atgaagctaa atgctcgcgc
      901 aaaagcgaaa atctgtcgat gtgccaatcg ttttcggcaa aaggaatcgt cgtttgcaga
      961 aggattttta tcggcttttt cataaatttt atgaaactgc gaatcggcgc ggataagata
     1021 aatctgaaat ctcggatttg aaatttcaaa tgccgggctt tgcctgcccg ctggtggtta
     1081 ataattgctt tcaaacagcc ggcagcgttt cgtaaaacat atcgtccacg tagcaataac
     1141 cccattcttc gcccggttcg aaggatttga taatcggatg agtgctggcg tgaaaatgtt
     1201 tggtcgcgtg tctattcggc gaattgtcgc agcagccgac gtgcccgcac gtttcgcaga
     1261 ggcgcaggtg aacccacctg ccgcccgttt tcaaacaatc ttcacagccg tccgcgctcg
     1321 gcgtcaccgc tttaatttcg ctcagatgct cgcatttgct catagttttt aaagtaaaaa
     1381 agtaaaaaag taaaaaaatg aaaaaggtta aaattgaaaa agtgaaaaag caatcattgc
     1441 tactcgtcaa caaacttttt cacgccttca cacgtccgct tttccacctt catcttaccg
     1501 cttcgccccg ctcatttcca accgatttcc gaggcggcgc gaatctttcg tcgagcggaa
     1561 caaacagggc gcgcatttcc tcggcggcga cctttacgat tcgcttcgta taagtctttt
     1621 cgcgcagact tccggcgaac gctaaaattt tcggttgttt tatcctcaga aagctctccg
     1681 gaattttatc ggaataataa cagtttgcca cgaccggcag aacgcggcaa tcggcgtttc
     1741 tcaaccgctg caaaaaattt tctgcaggaa tcgaaattta tgagagaatg attagtcaat
     1801 tcgtaatttt gaaattgaat tttacttcaa ggggtgacga gcgtgggaaa taaattgtct
     1861 ttgaaatgtt taaccgtttt attaatttgt ggttgttttg cgttttcggg cgcggcgcag
     1921 cagacgccgg ttgacgcttt gaaccggcgg caggaaagta aagaaactgc cgttacggaa
     1981 aaagccggag aagcgaagat aactgaaaaa atcggtaaat tcacggaatt ttctcctgcc
     2041 gcgaaagaaa aattcggctt tcctttatct ccaaaagaaa agccgcagcc gcagaaattt
     2101 gattttcccg aaccggaaaa tcagacggaa aaaaaagctc aaattgatgc tcaaaataat
     2161 cgaaatcggt gggttctgcc gcgcggcacg attgaatacg gcgccgaaat cggttacgcc
     2221 aacgaaatcg ccacctggat gagcggtccg aaatattatg atattcgcgg tcaccggcat
     2281 cttttaggct cgtttcgatg gggcagaatt ctcggcaata aaggaatcgt aacggttagc
     2341 tgggcggtcg aatttgttcc gataaacatc gccatcggaa acgaagtcga taatccgcgc
     2401 tttatgcccg gctcgacgac tgaaacgccg acaaagcgtg aaaacacttt cggcgtcggc
     2461 attaatccgg cgagttttcg ctttatcttt tttccgaaat ggcgactgcg accgcagctt
     2521 ggcgcgggca tcggtttgac gcgtcatttc aaccgcgttc cgaccatcga cggaaccaag
     2581 ggaaacgtcc acatcgattt tcaaatcggc gggcagtata tgatttctga aaggcgcgct
     2641 ttgaatttcg ggtataaata ttatcatctt tcgaatgtgt atctggcgca aagaaatccc
     2701 ggttataatg tgaatatgtt tttcgtcggg tattcgattt tcaaaagatg ataaaaccgt
     2761 ttcaaaatct gtcgccgcgc attcaccgga cggcttttat cgccgaaaac gcggtgatta
     2821 tcggcgatgt cgaaatcggc gaaaactcca gcgtctggta cgggtcgatt ctcagaggcg
     2881 acgtcaatca tatacgcatc ggcgcgcgga caaacgtgca ggacgcctgc gttctgcacg
     2941 tctccgccgg aacgcacccg accgttctgg aagatgaagt gacgctcggt catcgcgtca
     3001 cgctgcacgg ctgttacgtc gaaaccggct gcttaatcgg catcggcgcg attgtcatgg
     3061 acggcgcgcg cgtcggcaga aattcattaa tcggcgcggg cagcctcgtc acgccgaata
     3121 ccgagattcc gccgcgctcg ctggttctgg gcagcccggc gcgcgtcaag cgcgaattgt
     3181 cggatgcgga agtaaaagat ttggaaaaat tctggcataa ttacgtttca tacagccgga
     3241 tttacaaagg cgagccggtc tgagattttt gctcgccgga ttaaaaacaa ggggaaattg
     3301 gcataatgct tggtatcttg agactcgccg tgggcagtct gtttttaatt ttgaccgttt
     3361 tattttttac ggtttcggct cagcagacaa acgattcgct caaagaagat ttgcaaagcg
     3421 aaacagcctt tgccgaatcg actcgaacgg tgattgaaaa agaatctttc ataaaaagag
     3481 aagcaaggtt tttcacgcca gccgaaaatc gtctcatctc cccaaaatcc gtcgaaaccg
     3541 gcgcagacga gccgcaggcg tcaaccgaac gctccgttta ccgggcgcgg cgcggggcgc
     3601 gcgaatacaa ttttgaaatc ggcgtttcgc cgtttgagcc gacgaatttt aacgggcgta
     3661 aggaattcaa caccgccgga agaaaattcg gcacaataga ttttcgcatc ggtcgcgttc
     3721 tcggcacgag caagggaatt acctacacct actttttcgg tttttctccg ctggtcgtcg
     3781 cgctgaaaaa cgaagtgcgg aatccggctt acatctcgcc ggcggcgacg ccgaatgtct
     3841 accggacgcg gcgcgaaact tcgtatgcct tcgggtttac gccggcaaat tttcgcttca
     3901 cgtttttccc gggcagtcgt atcaaaccgt atgtgcaggg cggcgcgggc gttcttttct
     3961 tcaataagcc gataccgatt cccgaaagcc ggcgtatgca gtttatgggc gacctcggcg
     4021 gcggatttat cgttcatttg aaaaatccga agcagtttct cagtttcggc taccggtatt
     4081 ttcatatttc aaacggcaat atcggcggga aaagatggaa tccgggctac aacgcgaaca
     4141 ttttctacat cggattttcg tttttcaaat agtcggcttt cgtcaaaaac gaaaaattga
     4201 tttatgctta taacgcacgt cctagcccgc gtgcgttttt tactttccaa aacattttta
     4261 cggagaaact ttaatgcgaa aattttttgc cgcttttgcc tgcttgagca gtctttttat
     4321 tattattgcg gtttgccctt cggcgatttt tacacaggat ttgacgagcc ttcagaaaaa
     4381 agccgtgacg gcggaaaccg gcgtcgtctc gacgccgcaa acgtccggca cgagtgattt
     4441 tgacgagaaa aacgagcttt ccgtctgggg cggattcgcg cccgatatgc cgcgcgtttt
     4501 cggcggcgcg cgcccgtcca gttacggtca gctggcggtt cgttattcgc ggcgcatcgc
     4561 caccagcgac agcctcgcgc tcaaatacac gattgatttt attccgctcg ccgtcatcaa
     4621 ttacgaccgt cgcccgatta ttcaaaccgc gccgaatact tttacagtcc agagcaacaa
     4681 cacgaccaat tacgctttcg gcttgacgcc cgtcggactt cagctgcatt ttcgccgcaa
     4741 agctaaaatc cagccgttta tttcggcaaa cgccggtctg ctgattttca acaaaacgat
     4801 tcccgacgac cggagcgcgg tttttccgaa cctcaacggc aaacatctga attttacttt
     4861 agcgggcggc ggcggcgtcg attttctgac cgatgcgggc agaaccttta cggtcggctt
     4921 caagtttcac catatttcga acgcctcgac cggcaatatc aatccgggct ttgaccagaa
     4981 tttattttac gtcggctaca ctttcaaaaa gtggtaagcg cctagcagac aaagagcgga
     5041 aaaggcggaa acgctttttt aattacgaat tacgaattac gaatgacgaa tgaaggaaag
     5101 attcttccaa ttcgtaattc ttaatttgta attcgtaatt gtatagaagg ttcgcgtttc
     5161 cgccttttcg ctttataatt gcttagtcga gtaagctttt atattgaatc gctcctctga
     5221 cga
//
LOCUS       DASWNE010000283         2877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_467885, whole genome shotgun sequence.
ACCESSION   DASWNE010000283 DASWNE010000000
VERSION     DASWNE010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467885"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..387
                     /locus_tag="VGB68_05830"
     CDS             25..387
                     /locus_tag="VGB68_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048781.1"
                     /translation="MKKQNLVRAFLMAALLVGTFAVAGNDAYAQDTGNMGSGCCRGTS
                     QSDGGGTIGSGTLQSSGGMLGSGTATGGGGMIGSGTLTEDGGGTIGSGTRTGLIGPSG
                     GKTADSSFFYTLMRYFGF"
     gene            622..1704
                     /locus_tag="VGB68_05835"
     CDS             622..1704
                     /locus_tag="VGB68_05835"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY0048782.1"
                     /translation="MKINFEKRRIGQIILLIFAAHTILFIIELPQVYLYNAQSRSPIS
                     AWIQIARLGWGVYIWALVTPVVLWLGYLFPVTRKHLRRNLFLHLVFSVAAGIVQHFGY
                     NSGVLMFGLSTSETVRANFFNFALMLNFISSSMVRYATIIAIQQAYFYFQESQERAFR
                     LQQAELEVLKMQMHPHFFFNTLNAISALVYRSPKDADRTITQLSDLFRISLKKDKAQE
                     ISLKEELEFLQAYLQIHQTLMGKRLEVLWKIQPETLDALVPNLILQPLVENAVQHGVA
                     PLERGGQIEICSRRLNDRLLVQVCDNGRGLATDKIKNTGVGLSNTRARLKNLYADAHQ
                     FEITALPGGGGTSINLEIPFREQTKI"
     gene            1701..2462
                     /locus_tag="VGB68_05840"
     CDS             1701..2462
                     /locus_tag="VGB68_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY0048783.1"
                     /translation="MSIRTIIVDDVDLARERIKILLDDAEIEIVGEAANGREAIEAIR
                     DLQPDLVFLDVQMPQIDGFDVIETIGVEKMPAVIFVTAYDEFALRAFEINAVDYLLKP
                     FGEERLKKAVERARREIQRAEPAAEIEARLRRLLKEVKTEPKYLKRIPVKSARGATLV
                     STGEIDWINSAGHYLEIHAGRETHLVRERLSQIEQKLDPEIFIRIHRSIIVNLDRIKS
                     LHPLFNGDHLIILQNGQELNLSRTYHEKLMLLLSR"
     gene            complement(2471..>2877)
                     /locus_tag="VGB68_05845"
     CDS             complement(2471..>2877)
                     /locus_tag="VGB68_05845"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0048784.1"
                     /translation="AVIWGGLASGRLSGKVRRNQPAPENSRIAKGGTLGPAVAEEFLF
                     NLVDVLDEIAAENEKTVAQIALNWLLQRPSVASIVVGARDERQLKQNLEAVGWNLTKE
                     QVARLDKASETTPVYPYWHQRGFPQLNPPPTG"
BASE COUNT          700 a          746 c          769 g          662 t
ORIGIN      
        1 taaaattata aggagtaaat aaaaatgaaa aaacaaaatc tggttcgcgc ctttttaatg
       61 gcggcattgc tggtcggcac ttttgccgtt gccggaaacg acgcttacgc gcaggacacc
      121 ggcaatatgg gttccgggtg ctgccgcggc acatcgcagt ctgacggcgg cggaacaatc
      181 ggaagcggca cattgcagtc cagcggcgga atgctcggca gcggcacggc gaccggcggc
      241 ggcggaatga tcggcagcgg aactttgacc gaggacggcg gcggaacgat cggcagcggc
      301 acgcgcaccg gtttaatcgg tcccagcggc ggaaaaacgg cggattcgtc ttttttctac
      361 acgctgatga gatatttcgg cttctagtcc ggcatcgccg gttgcggcaa taataaaaaa
      421 acaaaagcga ctgttcagtt tgcccgactg aacagtcgct ttctaaattg tttttcggct
      481 taaaattgaa aaaggcgaat catttaaaat ccgagtgcaa acatcaaaaa ataaatttaa
      541 aattctccgc ttaagcccga atgattataa aataaaagtt gattcgtttg cccggcgacg
      601 gatgcaaaac cgaaaaattt tgtgaaaata aatttcgaaa agcggcgcat cgggcaaatc
      661 atcctgttga tttttgccgc ccacacaatc ctttttatca tcgaactgcc gcaggtttat
      721 ctttataacg cgcagagccg cagcccgatt agcgcgtgga tacagattgc gcgtctgggc
      781 tggggagttt acatctgggc gctggtgacg ccggtcgttt tatggctcgg ctatcttttt
      841 cccgtcacgc gaaaacacct tcggcgcaac ttatttttgc atctggtttt cagcgtcgcc
      901 gcgggaatcg ttcagcattt cggctacaat tcgggcgtgt tgatgttcgg gctttcgact
      961 tctgaaaccg tccgcgcaaa ctttttcaac tttgctttaa tgctcaattt tatttccagc
     1021 tcgatggtgc gctacgcgac gattatcgcc attcagcagg cgtattttta ttttcaggaa
     1081 tcgcaggagc gcgcctttcg tttgcagcaa gccgagctgg aagtgttgaa aatgcagatg
     1141 cacccgcatt ttttctttaa tactttgaac gccatctcgg cgctggttta ccgctcgccg
     1201 aaagacgccg accggactat cacgcagctc agcgatttgt tcagaatctc tctgaaaaaa
     1261 gacaaggcgc aggagatttc gctcaaagaa gagctggaat ttttgcaggc gtatttgcag
     1321 attcaccaga ctttgatggg caaaaggctg gaggttttgt ggaaaatcca gccggagacg
     1381 ctcgatgcgc tcgtgccgaa tttgattttg cagccgctcg tcgaaaatgc cgttcaacac
     1441 ggcgtcgcgc cgctcgaaag aggcgggcaa atcgagattt gcagccgtcg cctgaacgac
     1501 cggctcctgg tgcaggtgtg cgataacggg cgcgggctgg cgacggataa aataaaaaat
     1561 accggcgtcg ggctttccaa tacgcgggcg cggctcaaaa atctttacgc cgacgcgcat
     1621 caattcgaga ttacagcctt gcccggcggc ggcggaacga gcatcaatct ggaaattccg
     1681 tttcgggagc agacgaaaat atgagcatta ggacgattat cgttgacgac gtggatttgg
     1741 cgcgcgagcg catcaagatt ctgctcgacg acgccgagat tgaaatcgtc ggcgaagcgg
     1801 caaacgggcg cgaagccatc gaagccatcc gcgatttaca gcccgacctt gtttttctcg
     1861 acgtgcagat gccgcaaatc gacggcttcg acgtcatcga aacaatcggc gtcgaaaaaa
     1921 tgccggcggt gattttcgtg accgcttacg acgagttcgc gctgcgcgcc tttgagataa
     1981 atgccgtcga ttatctactt aaacccttcg gcgaagaacg tttaaaaaaa gccgtcgaaa
     2041 gagcaaggcg cgaaattcag cgcgcggaac cggcggctga gattgaagcg cgcctgcgcc
     2101 gtttattaaa agaagtcaaa accgagccga aatatctcaa gcgaattccg gtcaaatccg
     2161 cgcgcggcgc gacgctggtt tcgaccgggg aaatcgactg gattaactcc gccggacatt
     2221 atctggaaat tcacgccggg cgcgagacgc atctggttcg cgagcgcctg tcgcaaatcg
     2281 agcaaaaact cgacccggaa attttcatcc gcattcaccg ctcgattatc gtcaacctcg
     2341 accgcataaa atctcttcac ccgctcttta acggcgacca cctgattatt ctgcaaaacg
     2401 gtcaggagct taatctgagc cgcacctatc acgaaaaatt aatgctgctt ttgtcgagat
     2461 agaaaagaaa tcagccggtc ggcggaggat ttagctgcgg aaatccgcgt tgatgccagt
     2521 atggataaac cggcgtcgtt tcgctggctt tgtcgagccg cgcgacctgc tctttcgtca
     2581 gattccaccc gacggcttcc agattttgtt tcaattgccg ctcgtcgcgc gcgccgacga
     2641 cgatgctggc gacggaaggg cgctgcaaaa gccagtttaa agcgatttgc gcaacggttt
     2701 tttcattttc ggcggcaatt tcgtccaaca cgtccacgag attaaaaaga aattcttcgg
     2761 cgacagccgg acccagcgtg ccgcctttgg caatccggct gttttccggc gcgggctggt
     2821 tgcggcgcac ttttccgctc aagcgtcccg aagccaagcc gccccagata acggcgc
//
LOCUS       DASWNE010000284         2315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_80289, whole genome shotgun sequence.
ACCESSION   DASWNE010000284 DASWNE010000000
VERSION     DASWNE010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80289"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..694
                     /locus_tag="VGB68_05850"
     CDS             50..694
                     /locus_tag="VGB68_05850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0048785.1"
                     /translation="MDYSTAWQDKNFMPDADAESAEIAARAENSDRRLVELVLAGDET
                     AFEQIFERYKRLAAAIASRYFRRPEQIEEIIQISFTKVYFELKNFRGAHDASLASWLA
                     RITTNACLDALRSQNRKSENLLCELSEPEIEILFAAAPTVVENTEKSLVERDLAEKLL
                     SRLAADDRAILQMLDAEEMSVSEVAEITGWSCSKIKIRAFRARNALRKVLRKFL"
     gene            707..1456
                     /locus_tag="VGB68_05855"
     CDS             707..1456
                     /locus_tag="VGB68_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048786.1"
                     /translation="MKRLLERRMKKRDLQNEKLDRIGRKLLEAGRVPDKELNKIVSSP
                     RLFEAIRAGIKAEQRARKSKTFFGDWRNLLVWNRQKTAAALAVLLFLALGLIVFTKQD
                     APTLAEKTAPPQIRLPLAPVDIPSPAATVEDLPEIEKTKNSERQNRASFQKAVFKNVT
                     AALPKRAPRRKNPVKRQTASEPEGEFYALTYVENPGEAGEDLRIIRAELSRSSLFALG
                     VNLPIENESEKIKTDLLVGADGVAKAIRFVK"
     gene            1488..>2315
                     /locus_tag="VGB68_05860"
     CDS             1488..>2315
                     /locus_tag="VGB68_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048787.1"
                     /translation="MKYLFLILLCVTALTVTATAQQVIAPKATAEIIAGSSTTIVKGA
                     PFSAEAISESTQVLADGNKISRTFTVRMYRDGEGRFRREEVPATGGAVGTFGGVRQMI
                     SIFDPVANYRFFLDPSKKTARRMSILPGSAEGAVIVSGQPMSPALRAQIETNAVQRTN
                     IVVLPNVASTGGTGKSESLGTRSFEGLEAEGTRTTTTIPAGAIGNERPIELVYEKWYS
                     KDLQLIVYSKHSDPRFGEQIYRLDNIDRGEPDRSLFAVPSDYKLATEPRFNVVTPRPV
                     "
BASE COUNT          592 a          613 c          634 g          476 t
ORIGIN      
        1 ggcgtctttt ggtgcgaccg gtattcaaat atcaatccga aaactcaaaa tggattattc
       61 gacagcctgg caagacaaaa attttatgcc cgatgccgac gccgaaagcg ccgaaattgc
      121 cgctcgcgcc gaaaattcgg acagacgtct ggtcgagctc gttctggcgg gcgacgaaac
      181 ggcttttgaa cagattttcg agcgctataa acggctcgcc gccgccatcg ccagccgcta
      241 tttccggcgt cccgaacaaa tcgaagaaat tattcaaatc agttttacaa aggtttattt
      301 cgagctgaaa aattttcgcg gcgcgcacga cgcttcgctg gcgagctggc tggcgcgcat
      361 tacgaccaac gcctgtctgg acgcgcttcg gagtcaaaac cggaaatcgg aaaatctgct
      421 ctgcgaatta agcgaaccgg aaatcgaaat tttatttgcc gccgcgccga cggtcgtcga
      481 aaacacggaa aaatctctgg tcgagcgcga tttggcggaa aagcttctgt cgcgcttggc
      541 ggcggacgac cgcgcgattc tgcaaatgct cgacgccgag gaaatgagcg tcagcgaggt
      601 tgcggaaatc accggctggt cgtgctcaaa aatcaagatt cgcgcatttc gcgcgcgcaa
      661 cgcgctgcgg aaagtgctgc ggaaattttt gtaactgaag cgtggaatga aacgtctttt
      721 ggagcgaaga atgaagaaac gggatttaca aaacgaaaag ctcgaccgaa tcggacgcaa
      781 attgctggaa gccgggcgcg tgccggataa ggagctgaac aaaatcgtca gttcgccgcg
      841 gcttttcgaa gcgataagag ccggaatcaa agccgaacag cgcgcgcgca agtcgaaaac
      901 attttttggc gattggcgga atttgctcgt ttggaaccga caaaaaaccg ccgcggcgct
      961 ggctgtttta ttgttcctcg cgctcggctt gattgttttt acaaaacagg acgcgccgac
     1021 actggctgaa aaaaccgctc cgccgcaaat ccgattgccg ctcgcgcccg tcgatattcc
     1081 gtcgccggca gcaaccgtcg aagatttgcc cgaaattgaa aaaacaaaaa attctgagcg
     1141 ccaaaatcgc gcgagcttcc aaaaagcggt ttttaaaaat gtaaccgccg ctttgccgaa
     1201 acgcgcgccg cgcagaaaga atccggtgaa acggcaaacc gcaagcgaac ccgaaggcga
     1261 attttacgcg ctcacatatg tcgaaaatcc gggcgaagcc ggagaagatt tgcggattat
     1321 tcgggctgag ctttcgcgct cttcgctgtt cgcgctcggc gtcaatctgc cgattgaaaa
     1381 cgaatccgaa aaaatcaaaa ccgatttgct ggtcggcgcg gacggcgtcg ccaaagcgat
     1441 tcgatttgta aagtaatttt atagtctata gtttataagg agaaaaaatg aaatatttat
     1501 ttttaatttt actgtgcgtc acggctttga ccgtgacggc gacggcgcag caggtaattg
     1561 cgccgaaggc gacggcggaa atcatcgccg gcagctcgac gacaatcgtc aaaggcgcgc
     1621 cgttttcggc tgaagcaatc agcgagagca cgcaggttct cgccgacggc aataaaatat
     1681 cgcgcacttt tacggttcgt atgtatcgcg acggcgaagg tcgttttcgc cgcgaagaag
     1741 tgcccgcgac gggcggtgcg gtcggcacat tcggcggcgt ccggcagatg atttcaattt
     1801 tcgaccctgt cgccaattac agatttttct tagacccgtc gaagaaaacc gcgcgccgaa
     1861 tgagcattct accgggaagc gccgaaggcg cggtcatcgt cagcggtcag ccgatgagcc
     1921 ccgccctgcg cgcgcagatt gagacgaatg ccgtccagag aacgaatatc gtcgttttgc
     1981 cgaacgtcgc ctcgacaggc ggaaccggca aatcagaatc gctcggcaca cgcagttttg
     2041 aaggcttgga agccgaaggc acgcgcacca cgacgacaat tcccgccgga gccatcggca
     2101 acgagcgtcc gattgagctt gtttacgaga aatggtattc gaaagatttg cagttaatcg
     2161 tttactccaa acacagcgac ccgcgcttcg gcgagcaaat ctaccggctc gataatatcg
     2221 accgcggcga gcctgaccgt tcgcttttcg cagtgccctc ggactacaaa ttagctaccg
     2281 agccgaggtt taacgtcgtc acgccgagac ctgtc
//
LOCUS       DASWNE010000285         5892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_433030, whole genome shotgun sequence.
ACCESSION   DASWNE010000285 DASWNE010000000
VERSION     DASWNE010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5892
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433030"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(315..3575)
                     /locus_tag="VGB68_05865"
     CDS             complement(315..3575)
                     /locus_tag="VGB68_05865"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY0048788.1"
                     /translation="MNKIVLIILILLVASIAGFAQGDRPMLFRQPTMNKTDIVFVYGG
                     DLWRVARAGGNAERLTSGTGNESNPYFSPDGNWIAFTGEYDGNIDAFVIPASGGEPRR
                     ITYHPGADTVVGWTPDGSRVVFLSGRAAGMPLPKMYTMPVTGEGLPTELPFPMAGGVA
                     SFSPDGSRLAYMPLGAAFQQWKMYRGGRTTKIWIGNLSDSSVEEIPHPNSNDFTPQWV
                     GDRIYFLSDRNGRNVSLYSYDTKTKKVAPAVNNTGLDLKTFTAGADGVVYEQFGSVNV
                     YDPASGKTNKVNIALSGDLPQVRPRYEKVAQRVQNVAISPSGMRAVFEARGEILSVPV
                     EKGNARNLTNTSGVAERDPAWSPDGRWIAYFSDEGGEYALHLRDQTGMGEVKKISLGN
                     PSSYFYSPRFSPDSKKILFTDKRLNVLYLDIEKGAIVKVDTNTYENPFPVLDPNWSPD
                     SRWIVYTKQLKNRLCAVHVYSLETAKTAQVTDGLSDARYAAFDKNGKYLYFTASTDNG
                     PTTGWLDMSSFPFQTTRSVYAVVLKSTDPSPLSPESDEEKIEESKPQTPRAPGAKPEP
                     VTVTIDLDKINQRIVALPIAARNYQGITTGRAGTFFLGEAIPAIGTALTPQQGVTVHR
                     FDMDKRRAEKILDGVTAFDVSANGEKMLFGQAGGRFTIASTVQPLKPGEGVLNVGEME
                     VYVDPRAEWQQMYREAWRIQRDFFYDPTFHGVNIRLMEDRYRPFLESVATRSDLNYLF
                     QEMLGNLTVGHHNSGGGDIPQPTRYTGGLLGADYTIENGRYRFAKIYDGENWNPGLIA
                     PLTQPGVNVREGEYLIAVNGREVRAADNIYSFFQQTAGKQIIIRVGANADGRDARQVT
                     IVPVANEGGLRNLAWIEGNRRKVYELSGGKLAYVYLPNTGGAGYTNFNRYYFAQIDRE
                     GAVIDERFNGGGSAADYMISYMSRPLMNYWSTREGEDFATPVASIFGPKTMIINEYSS
                     SGGDLLPFLFRQNKLGPLVGKRTWGGLVGIYTYPVLIDGGSVTAPRVAFRNPQGELDV
                     ENKGVAPDIDIDLDPKMWRAGRDVQLEKAVEVTLAEIKRNPPRKPAKGAFPNYAKP"
     gene            complement(3684..>5892)
                     /locus_tag="VGB68_05870"
     CDS             complement(3684..>5892)
                     /locus_tag="VGB68_05870"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY0048789.1"
                     /translation="DGSPGFFHTPVWSPDSRKIAYNRQDVSLWYVDVEKGANVKVDTN
                     TFADFDNVLEPSWSPDSKWITYVKQLDNRLRAVYLYSVDKNQRNQITDGLGDARHIVF
                     DKSGKYVFFTASTNVGPTISFADLSGIAHQTSRNVYAVVLRNDIPSPLAPESDEEKVQ
                     PERKDEPPVVVPTVTPTPAPTATPTAMPAENPTPAPSPTPAEQSKPAASKQLEPTRID
                     LEGIDQRVISVTQIPARNYNGLYAGKAGILYVTEIPVAPQTNPPFGATLHKFDLEKRK
                     FEEVKTGVSNFRVSANGEKMLYLAGVWNLVSTTVPTRPGEGIVKTADMEVYVDPRVEW
                     RQMFNEIWRGERDFFYDPNTHGLDIEKAKRLYAPYVEAVVHRDDLNYLFREMLNQIGV
                     GHMFIGGGDQPRPAFVPVGLLGADYKIENGRYRFARVYNGENWNPQLRAPLTAPGVNV
                     RLGEYLLAVNGREVKSTDNVYQFFESTANRQIVIRVGASPDGKGAREVTVVPTANEGG
                     LRNLAWIEDNRRAVDRLSGGRLGYVYIPNTGGPGYTSFNRYFFAQTNKQGAVIDERFN
                     GGGLLADYVVEYLNRQQLAKIAFRDGKDWNVPAGAIYGPKAMLINELAGSGGDAMPWF
                     FKKLNVGPLIGKRTWGGLVRASGIPTLMDGGSIRAPNGAVYGLNGEWEVENVGVAPDI
                     EVEYDPALWRQGRDPQLEKAIEYLMAELQKNPPKEYKRPAYPNLHKDSGLGRP"
BASE COUNT         1190 a         1695 c         1635 g         1372 t
ORIGIN      
        1 agacgcggag gaagaattaa cagggataac aggatgaaca ggataagaaa gataatttct
       61 tttttattat ccctgtttat cctattatcc ctgttaattc tctttcaaaa atctccgcgt
      121 ctcggcggtt tatttttctt atccgatact gacgttagaa taatccaatt taccgtttaa
      181 tcaaatgcca gaaaatgcgc ccgcgaaacg agcgaaccca cacgaaaatt atttttagtt
      241 tttcgtatct ttcgcgcgtt tcgcgggcga aaccggcggg gcttttataa acagccagtc
      301 gatttactca cattttacgg tttggcgtaa ttcggaaaag cgcctttcgc cggtttgcgc
      361 ggcggatttc ttttgatttc cgccagcgtt acctcgaccg ctttttccag ctgaacgtcg
      421 cgtccggcgc gccacatttt cgggtcgagg tcgatgtcga tgtccggcgc gacgcctttg
      481 ttttcgacgt cgagctcgcc ctgcggattt ctgaaagcga cgcgcggagc cgtcacgctg
      541 ccgccgtcaa tcaaaaccgg gtaagtgtaa atgccgacca gcccgcccca ggtgcgtttg
      601 ccgaccagcg gtccgagctt gttctggcga aacaggaacg gcagaaggtc gccgcccgag
      661 ctggaatatt cgttgataat catcgtcttc ggaccgaaaa tggaagcgac cggcgtggcg
      721 aaatcctcgc cctcgcgcgt cgaccagtaa ttcatcagcg ggcggctcat atagctgatc
      781 atataatcag ccgcgctgcc gccgccgtta aaacgctcgt caatcaccgc gccttcgcgg
      841 tcaatctgcg cgaaatagta gcggttgaaa ttcgtatagc ccgcgccgcc cgtgttcggc
      901 agataaacgt aagcgagctt gccgccgctc aattcgtaaa ccttgcggcg attgccttca
      961 atccacgcca gatttcgcaa gccgccctca ttcgcgaccg gaacgattgt cacctgccgc
     1021 gcgtctcgcc cgtccgcgtt tgcgccgacc ctgatgatga tttgttttcc ggcggtttgc
     1081 tggaaaaagg aataaatatt gtcggcggcg cggacttcgc gaccgttgac ggcgattaaa
     1141 tattcgcctt cacgaacgtt gacgcccggt tgggtaagcg gcgcgattaa gcccggattc
     1201 cagttttcgc cgtcgtaaat tttggcgaag cgatagcgcc cgttttcaat cgtgtaatcc
     1261 gcgcctagaa gcccgcccgt gtagcgcgtc ggctgcggga tgtcgccgcc gcccgaattg
     1321 tggtgtccga ccgtcagatt gccgagcatt tcctgaaaca ggtaattcaa atcgctgcgc
     1381 gtggcgaccg attccagaaa cgggcggtaa cggtcttcca tcagcctgat gttgacgccg
     1441 tgaaaggtcg ggtcgtagaa aaaatcgcgc tggatgcgcc aggcttcgcg atacatctgc
     1501 tgccactcgg cgcgcgggtc gacgtaaact tccatctcgc cgacattcaa aacgccttcg
     1561 cccggcttta aaggctggac ggtcgaagcg attgtaaagc gtccgcccgc ttgcccgaac
     1621 aacatctttt cgccgtttgc cgaaacgtcg aacgccgtca cgccgtcgag aattttttcg
     1681 gcgcggcgct tgtccatatc gaatcggtga accgtcacgc cctgctgcgg cgtcaacgcc
     1741 gttccgatag cgggaatggc ttcgccgaga aaaaatgttc cggctctgcc cgtcgtgatg
     1801 ccctgataat tgcgcgccgc aatcggcagc gcgacgatgc gctggttgat tttgtccaaa
     1861 tcaatcgtca ccgtgacggg ttcgggtttc gcgcccggcg cgcgcggcgt ctgcggtttg
     1921 gattcttcga ttttttcctc gtcgctctcg ggcgaaagcg gcgacgggtc ggtgcttttc
     1981 aaaacgaccg cgtaaacgct gcgcgtcgtc tgaaacggaa agctcgacat atcgagccag
     2041 ccggtcgtcg gaccgttatc ggtgctggcg gtgaaataaa ggtatttgcc gtttttatca
     2101 aaagcggcgt agcgcgcgtc cgacaagccg tccgtgacct gcgccgtttt ggcggtttcc
     2161 agcgaataaa cgtgaaccgc gcaaagacga tttttcaatt gtttcgtgta aacaatccaa
     2221 cggctgtccg gcgaccagtt cgggtcgagc acgggaaacg gattttcgta ggtgttcgta
     2281 tcgactttga cgattgcgcc cttttcgatg tcgagataaa ggacgttcaa acgcttgtcg
     2341 gtaaacaaaa tcttcttgct gtcgggcgaa aaccgcggcg aataaaaata agaagacgga
     2401 ttgccgaggc tgattttttt cacttcgccc attcccgtct ggtcgcgcag gtgcagagcg
     2461 tattcgccgc cctcgtcgga aaaatacgca atccatctgc cgtcgggcga ccacgccggg
     2521 tcgcgttcgg cgacgcccga cgtgttcgtc agattccgcg cgtttccctt ctcgaccggc
     2581 acgctcagga tttcgccgcg cgcttcgaaa accgcgcgca taccggaagg cgaaatggcg
     2641 acgttctgca cgcgctgcgc gactttttcg taacgcgggc gcacctgcgg caaatcgccg
     2701 ctcagcgcga tattgacttt attcgttttg cccgacgccg gatcgtaaac gtttaccgag
     2761 ccgaactgct cgtaaacgac gccgtccgcg cccgccgtaa acgttttcaa atcaagcccg
     2821 gtattattaa ccgccggcgc tacctttttc gtcttcgtgt cataagaata aagcgacacg
     2881 ttgcgcccgt tgcggtcgga gagaaaatag atacggtcgc cgacccattg cggcgtaaag
     2941 tcattggaat tcggatgcgg gatttcttcg acgctcgaat cgcttaaatt tccaatccag
     3001 attttcgtcg tgcgcccgcc gcgatacatt ttccattgct gaaaagccgc gccgagcggc
     3061 atatatgcga gccgcgagcc gtcgggcgag aacgaggcga cgccgcccgc catcggaaac
     3121 ggcagctcgg tcggcaagcc ttcgcccgtc acgggcatcg tatacatttt cggcagcggc
     3181 attccggcgg cgcgtcccga aagaaaaacc acgcggctgc cgtccggcgt ccagccgacg
     3241 accgtgtccg cgcccggatg atacgtgatg cgccgcggct cgccgccgct cgcgggaatg
     3301 acgaaagcgt cgatgtttcc gtcgtattcg cccgtgaaag caatccagtt tccgtcgggc
     3361 gaaaaatacg ggttcgattc attgcccgtt ccgctcgtca gacgctcggc attgccgccg
     3421 gcgcgcgcga ctctccataa atcgccgccg tagacgaaaa cgatatcggt tttattcatc
     3481 gtcggctggc gaaacaacat cggtctgtcg ccctgcgcaa agcctgcgat tgaagcgacc
     3541 agtaaaatca aaataattaa aacgatttta ttcattatct ctcctgtcca aactcattaa
     3601 aggattcaag attcaggatt caggattcag gattacagtt tgaattccga atcttgaacc
     3661 ttgaatcttg aatcccgcat taattacggt ctgcccaaac ccgaatcttt gtgcaaattg
     3721 ggataagccg ggcgcttgta ttccttcggc gggtttttct gcagttccgc catcaaatat
     3781 tcgattgctt tttcgagctg cgggtcgcgt ccctgccgcc agagcgccgg gtcgtattcg
     3841 acctcgatgt cgggcgcgac gccgacgttt tcgacttccc actcgccgtt taagccgtaa
     3901 accgcgccgt tcggagcgcg gatgctgccg ccgtccatca gcgtcgggat gcccgaagcg
     3961 cgaaccagtc cgccccacgt gcgtttgccg ataagcggac cgacgttcag ttttttgaaa
     4021 aaccacggca tcgcgtcgcc gcccgaaccg gcaagctcgt tgatgagcat cgctttcggt
     4081 ccgtaaatcg cgcccgccgg aacgttccag tctttgccgt cgcggaaagc gattttggca
     4141 agctgctggc ggttcagata ttcgacgacg taatcagcca gcagaccgcc gccgttaaag
     4201 cgctcgtcaa tcaccgcgcc ctgtttattc gtctgcgcga aaaagtagcg gttaaagctc
     4261 gtataaccgg gaccgcccgt gttcggaata taaacataac cgagcctgcc gccgctcaat
     4321 ctgtcgacgg cgcgccggtt gtcttcaatc cacgccaggt ttcgcaaacc gccttcgttc
     4381 gccgtcggaa cgaccgtcac ttcgcgcgcg cctttgccgt cgggactcgc gccgaccctg
     4441 atgacgattt gcctgttggc ggtgctttca aaaaattgat agacgttgtc ggtcgatttg
     4501 acttccctgc cgttgacggc taataaatat tcgcccagac ggacgttgac gcccggcgcg
     4561 gtcaaaggcg cgcgaagctg cggattccag ttttcgccgt tgtaaacgcg ggcgaagcga
     4621 taacgcccgt tctcgatttt ataatccgcg ccgagcaagc cgaccggcac gaaagcgggg
     4681 cgcggctggt cgccgccgcc gataaacata tgcccgacgc cgatttggtt cagcatctcg
     4741 cggaaaagat aattcaaatc gtcgcgatgc acgaccgctt cgacataagg cgcgtataat
     4801 cttttcgcct tttcgatgtc cagcccgtgc gtgttcgggt cataaaagaa atcgcgctcg
     4861 ccgcgccaga tttcgttgaa catctgtcgc cattcgacgc gcgggtcgac gtaaacttcc
     4921 atgtcggcgg tcttgacgat gccttcgccg ggacgcgtcg gcacggtcgt cgaaaccaga
     4981 ttccagacgc ccgccaggta aagcattttc tcgccgttgg cggagactcg aaaattactg
     5041 acgccggttt tcacttcctc gaacttgcgt ttttccaaat cgaatttgtg cagcgtcgcg
     5101 ccaaacggcg gattggtctg cggcgcgacc ggaatttccg tgacatacaa aattcccgct
     5161 ttgcccgcgt agagcccgtt ataattgcgc gccggaatct gcgtgacgct gatgacgcgc
     5221 tggtcgatgc cttccaaatc gatgcgcgtc ggctcaagct gtttcgacgc agcgggtttc
     5281 gactgctcgg ctggcgtcgg gcttggcgcg ggcgtcgggt tttctgcggg catcgcggtc
     5341 ggggtcgccg tcggcgcggg cgtcggcgtg acggtcggaa cgacgacggg cggctcgtct
     5401 ttcctttcgg gctgaacttt ttcctcgtcg ctttccggcg cgagcggcga aggaatatcg
     5461 ttgcgcagaa cgacggcgta aacgttgcgc gatgtttgat gagcgatgcc ggaaaggtcg
     5521 gcgaagctta tcgtcggacc gacgttggtg ctcgccgtaa aaaagacgta ttttccgctt
     5581 ttatcgaaaa cgatgtggcg ggcgtcgccc aaaccgtcgg tgatttgatt tcgctggttt
     5641 ttatcgaccg aatacaggta aacggcgcgc aggcgattgt ccagttgttt gacgtatgta
     5701 atccacttgc tgtcgggcga ccacgaaggc tctaaaacgt tgtcgaaatc ggcgaaagta
     5761 ttcgtatcga ctttaacgtt cgcgcctttt tcgacgtcga cgtaccagag acttacgtct
     5821 tggcgattgt aagcgatttt cctgctgtcg ggcgaccaga cgggcgtgtg aaaaaatccg
     5881 ggcgagccgt cg
//
LOCUS       DASWNE010000286         4170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_256892, whole genome shotgun sequence.
ACCESSION   DASWNE010000286 DASWNE010000000
VERSION     DASWNE010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256892"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1737
                     /gene="mutS"
                     /locus_tag="VGB68_05875"
     CDS             <1..1737
                     /gene="mutS"
                     /locus_tag="VGB68_05875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEY0048790.1"
                     /translation="VTGMGARLLRTWLLRPSIKRSEIQTRLSAVSELTDSILRDKIRF
                     LLKEVSDLERLVGRLNLGTATPRDLLALNRSLSQTPNINQSLSDAQSLLLQVLSENIF
                     ELPEIQNLISRSISDEPPINLSDGGVIRQGFNAELDELREISQSAKQIIAGFEERERS
                     RTGIYNLKIKFNSVFGYFIEVSKGNAPRVPADYERRQTLTNAERFTTPQLKEWEEKVL
                     GAEERIKEIEAEIFYQIRAEVQKETRNLQSTARALATLDALQSLAETAARRDYVAPVL
                     HDGDEIEIKNGRHAIVEAFSRENFIPNDLYMNNSTDRLLIITGPNMGGKSTNLRQVAL
                     IQILAQIGSFVPASSVRLPILDRIWTRVGASDDLASGRSTFMVEMTETAAILHNATPR
                     SLILLDEIGRGTSTFDGLSIAWAVAEYLHDSPEHSAKTLFATHYHELTELAEKLAGAK
                     NYQITATEKDGEVVFLHKLEEGKASKSYGIAVAQLAGLPAKAIARAREVLEKLEKYEL
                     AVFAEQKKAASTDALEKAVNRASKSKLASQISLFAISNENVIDELRETDIDNFSPEEA
                     KKLLTEFKNRII"
     gene            2003..3958
                     /locus_tag="VGB68_05880"
     CDS             2003..3958
                     /locus_tag="VGB68_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048791.1"
                     /translation="MQISKQFRFYLLLCLLILTVVSASAQNRTVNAAQYKQLGGAAEE
                     ISAMRLPNPLSTGIRSKAAMIPIYASDDLREIEIPVDNPKEFKLMLLAPGASGWKIQA
                     ALPDEDFVNLRDSGLLQNVERTQTDFGMDGNQFPAEVFRLGAAREGVLRVKIDLPDSP
                     AASVKPLGYLVASSESMYRLYSYIDTFETLVGREIGINASLFDKKSNPETGAPNSLIG
                     SIRSSKVSIQTPEGEIVNIEMNDTGNGNLRAAFVPQTAGKYFVQITTEGTTPDGREFL
                     RTAEHGFAVAAENLRIKDAAKARLIDDLRWQIDLPVNNMKIGQKVIAHAEIWSNEQPV
                     AWIGGMAEGKGFDKRGVSISLALDSRWLLRVEAGGNYELRNVRLQDADNSIVLAKAEK
                     ISLSTVYVPDTARSFGNEITDEMRHGARPEMQTGNAVGGKLMLVHGYCSSDVWGAAAS
                     SGQFSNYVKFLDLNQNRSHDQFANLIRNFGASLPSFGIVAHSQGGAASLHLYTYYWSG
                     LDYATGGTRLIQSVGTPYQGTALAGNLALLGQIFGAGCGANNDMTYSGAAAWLSGIPS
                     WARAKVYYHTTSFTDVWYRYDYCNLATDPFLSDPDDGVVERAYAQLSGANNLGHKTGW
                     CHSSGMRDPSQTTDSSRNTNMNANAAR"
BASE COUNT         1038 a         1079 c         1096 g          957 t
ORIGIN      
        1 gtcaccggaa tgggcgcgcg gcttctgcgc acctggcttt tgcgaccttc gataaaacga
       61 agtgaaattc agacgcgtct gtcggcagtt tccgagttga ccgattccat tctgcgcgac
      121 aaaattcgtt tccttttaaa agaagtgtca gatttggaaa gattggtcgg gcgtttaaat
      181 ctcggaacgg cgacgccgcg cgaccttttg gctctaaacc gttccctttc gcagacgccg
      241 aacatcaatc agtcgttatc ggacgcgcaa agtcttctgt tgcaggtttt gtcggaaaat
      301 atttttgagc tgccggaaat ccaaaattta atttcgcgtt ccatctccga tgagccgccg
      361 atcaatcttt ccgacggcgg cgtgattcgt cagggcttta acgcggagtt ggacgagctg
      421 cgtgaaatca gccaatcggc gaaacagatt atcgccggtt ttgaagagcg cgagcgcagc
      481 cgcaccggaa tttataatct caaaatcaaa ttcaatagcg tcttcggcta ttttatcgag
      541 gtctcgaaag gcaatgcccc gcgcgttcca gccgattacg agcgccgcca gactttaacg
      601 aatgccgagc gttttacgac gccgcagctg aaagaatggg aagaaaaggt tctcggcgcg
      661 gaagagcgca tcaaggaaat cgaagctgaa attttttatc aaatccgcgc cgaagtccaa
      721 aaggagacgc gcaacctgca atcgaccgcg cgcgcgctgg cgacgctcga cgccctgcaa
      781 tcattagccg aaaccgccgc gcgccgcgat tacgtcgcgc cggttctgca cgacggcgac
      841 gaaatcgaaa tcaaaaacgg acgccacgcg attgtcgaag ccttttcgcg cgaaaatttt
      901 attccgaacg atttgtatat gaacaattcg accgaccgcc tgttgattat caccggtccg
      961 aatatgggcg gaaaatcgac caatctgcgg caggtcgctt tgattcagat tctcgcgcaa
     1021 atcggctcgt tcgttccggc atcgtccgtc aggctgccga ttctcgaccg catctggacg
     1081 cgcgtcggcg catccgacga tttggcttcg ggacgctcga cgtttatggt cgagatgacg
     1141 gagaccgccg cgattctgca caacgcgacg ccgcgcagcc tgattttgct cgacgaaatc
     1201 gggcgcggga cttcgacttt cgacggttta tcaatcgcct gggcggtcgc cgaatatctg
     1261 cacgattcgc ccgaacattc cgcgaaaacc cttttcgcga cgcattatca cgaattaacc
     1321 gagcttgccg aaaaactggc gggcgcgaaa aattaccaaa tcacggcgac ggaaaaggac
     1381 ggcgaagtcg tttttctgca caaactggaa gaaggaaaag cctcgaaatc ctacggcatc
     1441 gccgtcgcgc agctcgccgg actgcccgcg aaagcgattg cccgcgcgcg cgaagtgctg
     1501 gaaaaactgg agaaatatga attagccgtt ttcgccgagc aaaagaaagc ggcttcgacc
     1561 gacgcgctgg aaaaagccgt caaccgcgcg agcaaatcca agctggcttc gcagatttcg
     1621 ctgtttgcca tctccaacga aaacgtcatc gacgagctgc gtgaaaccga catcgacaat
     1681 ttttcgcccg aagaagcgaa aaaattattg accgagttta aaaacagaat catttagaaa
     1741 attatcgacc cagtttaaaa gcagaatcat ttaagcgaaa agcggtaaac ggttaagtgg
     1801 tgagttcgta gttttgaatt tcgagtttca aacaaaaaat cgatactttg aaattgcagc
     1861 ttaccgctta ccgtttaccg ctttccactt ttaccgctta ccgtttaccg atttattgtt
     1921 tataatcatc acgcttttca aaattttccc ttttatcaat cgcgtcatct ttctatttaa
     1981 ataaattttc ggggagattt gtatgcagat ttcaaaacag tttcgcttct acttgcttct
     2041 atgcctttta attttgacgg tcgtttccgc ttcggcgcag aaccgcacgg tcaatgccgc
     2101 tcaatataaa caactcggcg gcgcggctga ggaaatttcc gcgatgcgcc tgccgaatcc
     2161 gctttcgacc ggcattcgct cgaaagcggc gatgattccg atttacgcga gcgacgattt
     2221 acgcgaaatc gagattccgg tcgataatcc gaaagaattc aaattgatgc tgcttgcgcc
     2281 cggcgcttcg ggctggaaaa tccaggcggc tctgccggat gaagatttcg tcaatctgcg
     2341 cgacagcggc ttgctgcaaa acgtcgaacg cacgcagacc gatttcggaa tggacggaaa
     2401 tcagtttccg gctgaagttt ttcgtctcgg cgcggcgcgc gaaggcgttt taagagtcaa
     2461 aatcgatttg ccggattcgc ccgccgcaag cgtaaaacct ctgggttatc tggtcgcgtc
     2521 gagcgaatca atgtaccggc tttattcata tatcgacacg tttgaaaccc ttgtcggacg
     2581 cgaaatcggc atcaacgcat ctttgttcga taaaaaatca aaccccgaaa ccggcgctcc
     2641 gaattcctta atcggcagta tccgcagctc aaaggtttcg attcaaacgc cggaaggcga
     2701 aatcgtaaat atcgaaatga acgacaccgg caacggaaat ctgcgcgccg ctttcgtgcc
     2761 gcagaccgcc ggaaaatatt tcgtacagat tacgaccgag ggcacgacgc cggacgggcg
     2821 cgaatttctg agaaccgccg aacacggctt tgcggtcgcg gcggagaatc tgagaattaa
     2881 agacgcggcg aaagcccggc tgattgacga tttacgctgg cagattgatt tgccggttaa
     2941 taatatgaaa atcggtcaga aagtcatcgc tcacgctgaa atctggtcga atgaacagcc
     3001 cgtggcgtgg atcggcggaa tggcggaagg aaaaggcttc gacaaacgcg gcgtttcgat
     3061 ttctctggcg ctcgattcgc gctggctttt gcgcgtcgag gcaggcggaa attacgaatt
     3121 gcgaaacgtt cggcttcagg acgcagacaa ttcaattgtg ctggcaaaag ccgaaaaaat
     3181 ctctctttcg acagtttacg ttcccgatac ggcgcgaagc ttcggcaacg aaatcaccga
     3241 cgagatgcgg cacggcgcaa gaccggaaat gcaaacgggc aacgcggtcg gcggaaaact
     3301 gatgctcgtt cacggctatt gttcgagcga tgtgtggggc gcggcggctt cgtcgggtca
     3361 attttccaat tacgttaaat ttctcgattt aaaccaaaat cgctcgcacg accagtttgc
     3421 caatttaatc cgaaatttcg gcgcgtcgct tccgtctttc ggcatcgtcg cgcacagtca
     3481 gggcggcgcg gcgtcactgc atctctacac ttattactgg agcggtttgg attacgcgac
     3541 cggcggaacg cggctgattc aatcggtcgg aacgccgtat cagggcacgg cgctcgccgg
     3601 aaacctggcg cttttaggac aaattttcgg cgcgggctgc ggggcgaaca acgatatgac
     3661 gtacagcggc gcggcggctt ggctgtcggg cattccttcg tgggcgcggg cgaaagttta
     3721 ttatcacacg acttcgttta cggacgtctg gtatcgctac gattactgca atctggcgac
     3781 cgacccgttt ttgagcgacc cggacgacgg agtggtcgaa agagcttacg cgcagttgag
     3841 cggcgcgaat aatctcggac acaaaaccgg ctggtgtcat tcgagcggta tgcgcgaccc
     3901 gtcgcaaacg accgattcgt ctagaaatac aaatatgaac gccaacgccg cgcgataaaa
     3961 cagcagaggc gcataggaga ttcaaaccgg ataccaggtt gatttaaaac aaggaacgtt
     4021 cgagtttctt gtttatcccg cctatccggt ttgaatctcc atttgattaa atcacgctaa
     4081 ttaaaactgc aagaccgtcg atttgttatt cggtttgacg cgcacggata aagtgttgtc
     4141 ggcgttcaga gtcatttcga cgaagcggtt
//
LOCUS       DASWNE010000287         3778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_44342, whole genome shotgun sequence.
ACCESSION   DASWNE010000287 DASWNE010000000
VERSION     DASWNE010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44342"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..421)
                     /locus_tag="VGB68_05885"
     CDS             complement(65..421)
                     /locus_tag="VGB68_05885"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY0048792.1"
                     /translation="MITVANRIYVSAEYAEAFERVFRERAKLVDLMPGFVSNQVLRPV
                     NEGDPYVVFTIWNSREDFMNWVRSEEFIKGHAQSGTLPKEAYFKPNVLEMHEVLQDSA
                     RPDLQPEPRGGAFKMH"
     gene            complement(437..2386)
                     /locus_tag="VGB68_05890"
     CDS             complement(437..2386)
                     /locus_tag="VGB68_05890"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY0048793.1"
                     /translation="MTLETDDVKTFKEFGLIDELMKAIDDVGYETPSPIQLKTIPVLL
                     EGRDIVGQAQTGTGKTAAFALPGLQKIDTSTSRVQLLVLTPTRELAIQVAEAFHTYAK
                     HLGRVRVLPVYGGQSITQQIKHLRQGVQVIVGTPGRVMDHMRRETIDLTSLKMVVLDE
                     ADEMLRMGFEEDVEWILSHTPETRQTALFSATMPRQVRRIAEKYLNDPINIETERKTM
                     TVVNTKQFYVNVSEGQKIDALTRILEADSSPGEAVLIFHRTKIGADQLTEKLQARGYA
                     AEAMHGDMNQNQREAVIKRLKNGQVEIVVATDVAARGLDVEQITSVINYDMPGDTEVY
                     VHRIGRTGRAGREGKAILFVTPRQQRMKRDIENYTKQHIEPFRMPTQADVASRRVAIL
                     KERILDTLTKQDLELYLSLVEELAEECGCDIAEIAAAATFLAVGDKPLAVKVQPKAEQ
                     LVKQWNTDMTRLFIDAGRSHGVSAGDIVGAIANEGDVPGKAIGAIDIYDRFTLVDVPS
                     EFTEQVLEQMRNSRIRSQKANVRVADSSDASENTRKSFEKSPKSFEPERDSFEKPRKT
                     FEKSRDDYADKSGGDKPPRRKKTEGRENSFKQYEKPGSRKKTNSRPKIEAKTAKPKKK
                     LIDGKKKPKWTKEQRAERGKFPKSR"
     gene            complement(2601..3119)
                     /locus_tag="VGB68_05895"
     CDS             complement(2601..3119)
                     /locus_tag="VGB68_05895"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEY0048794.1"
                     /translation="MESRAKVLGHAAHPILIVFPLGLLATAVVFDVVYLATGTEQWSV
                     IAFWLIAAGIVGGLIAAVPGWIDWFAIPSGTRAKSVGLAHGVGNVVVLVLFAASWLFR
                     RDAGSGFEMAGAPGALALVCSFAGAGLALLTGWLGGELIERLGIAVHDGAHLDAPNSL
                     SGRPASENSKST"
     gene            3493..>3778
                     /locus_tag="VGB68_05900"
     CDS             3493..>3778
                     /locus_tag="VGB68_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048795.1"
                     /translation="MKIFTAILFFCLTAIVADVAGQAQMPNATLFAPGIVSTGFMETS
                     ATFTPDGKKVYFTRSDVQFSDNTIMQSTFKNGRWSEAQVASFSGVWRDSEP"
BASE COUNT          812 a         1042 c          934 g          990 t
ORIGIN      
        1 ccggagacaa acaagattag aagaataaag agttttcgta tgacgcagcg gtttcccttc
       61 cgccctagtg catcttgaaa gctccgccgc gcggttccgg ctgcaaatcg gggcgggctg
      121 aatcctgcaa tacttcatgc atttcaagca cgttcggctt gaaataggct tctttcggaa
      181 gagtgccgga ttgagcgtga cctttgataa attcttcgga gcgaacccaa ttcataaaat
      241 cctcgcggct gttccagatt gtaaaaacga cataagggtc gccctcattg accgggcgca
      301 gaacctgatt ggaaacgaat cccggcatca ggtcgaccag ttttgcgcgc tcgcgaaata
      361 ctctttcaaa agcctcggcg tattcggcgc tgacatatat gcgattggcg acagttatca
      421 ttttactttt tcctttttat ctcgatttgg gaaatttacc gcgctcggcg cgctgctcct
      481 tggtccactt cggcttcttt ttgccgtcta tcaatttttt tttgggcttg gcggttttcg
      541 cttcgatttt cggacgcgaa ttcgtctttt ttcggctgcc cggcttttca tattgtttga
      601 aggaattttc cctgccctcg gtttttttcc gccggggcgg tttgtcgccg ccggatttat
      661 cggcgtaatc gtcgcgcgac ttttcaaaag tttttcgggg cttttcaaaa ctatcgcgct
      721 ccggctcaaa gcttttgggc gatttttcga aagatttgcg cgtattttcc gaagcgtcgc
      781 tcgaatcggc gacgcgcacg ttggcttttt gactgcggat gcggctgttg cgcatttgtt
      841 cgagcacttg ctcggtaaat tccgaaggca cgtcgaccag cgtaaaacgg tcgtagatgt
      901 cgattgcgcc gatggctttt cccggcacgt cgccttcgtt ggcaatcgcg ccgacgatgt
      961 ctccggcgct gacgccgtga ctgcgtccgg catcgataaa caggcgcgtc atatccgtat
     1021 tccattgctt gacgagctgt tcggctttcg gctgaacctt gacggcgagc ggtttgtcgc
     1081 cgacggcgag aaacgtcgcg gcggcggcga tttcggcgat atcgcagccg cattcttcgg
     1141 ctaattcctc gaccagcgaa agataaagct ccaaatcctg tttcgtcaaa gtatccagaa
     1201 ttctttcctt taaaatcgcc acgcggcgcg acgcgacatc agcctgcgtc ggcattcgga
     1261 aaggctcgat gtgctgtttc gtataatttt cgatgtcgcg cttcatccgc tgctggcgcg
     1321 gcgtgacgaa taaaatcgcc tttccttcgc gcccggctcg acccgtgcga ccgatgcggt
     1381 gaacgtaaac ctccgtatcg cccggcatat cgtaattgat gaccgacgta atctgctcga
     1441 cgtccagccc gcgcgcggcg acatccgtgg cgacgactat ttcgacctga ccgtttttca
     1501 gtcttttaat aaccgcttcg cgctggtttt gattcatatc gccgtgcatt gcttccgccg
     1561 cgtagccgcg cgcctgtagt ttttcggtca actggtcggc gccgattttc gtgcggtgga
     1621 agataaggac ggcttcgccc ggagaagaat cggcttcgag aattctggtc aacgcgtcga
     1681 ttttttgtcc ttcggacacg ttgacgtaaa actgcttggt gttgaccacc gtcatcgttt
     1741 tgcgctcggt ttcgatattt atcgggtcat tgagatattt ttcggcgatg cgccgcacct
     1801 ggcgcggcat cgtcgccgaa aaaagcgccg tctgacgcgt ctcgggcgtg tgcgagagaa
     1861 tccattcgac atcttcctca aagcccatcc gcagcatctc gtcggcttcg tcgagcacga
     1921 ccattttcag gctggtcaaa tctatcgtct cgcgccgcat atggtccatt acgcgaccgg
     1981 gcgtgccgac aattacctgc acgccctggc gcagatgttt aatctgctgc gtgatcgact
     2041 gcccgccgta aaccggcaaa accctgacgc gcccgagatg tttcgcataa gtgtgaaaag
     2101 cttcggcgac ctgtatggca agctcgcgcg tcggcgtcag cacaagcagc tgaacgcggc
     2161 tggtcgaagt gtcgattttc tgcaaacccg gcagcgcgaa cgccgccgtt tttcccgttc
     2221 cggtctgcgc ctgcccgaca atatctctgc cttcgagcag caccggaatg gttttaagtt
     2281 gtatcggcga aggcgtttca taaccgacat cgtcgattgc cttcatcagc tcgtcaatca
     2341 gcccgaactc tttaaaagtt tttacgtcat ccgtctctaa agtcatagac acaattctat
     2401 actaaaagcg aataatggca cattccggcg gtttccatca aatattcccg ccaatgacgt
     2461 tcgaggcttt ccatcattcc ggtcaaatcg cgggcaagct ttcagtaaaa tgaaatgccg
     2521 cgacttcaaa cgccgctcaa gtcggcactc ggctgcttgc ctttcgggcg attcgttttt
     2581 ttgccaggcg tcgtatcgat ttacgtgctt tttgagttct cgcttgcggg gcgtcccgat
     2641 aaagagttcg gcgcatccaa atgcgcgccg tcgtgaaccg ctatgccaag acgctcaatc
     2701 agctcgccgc cgagccagcc ggtcagaagc gcaagtcccg cgcccgcaaa cgagcaaacg
     2761 agcgccagcg cgcccggcgc accagccatc tcgaaaccgc ttccggcgtc gcggcgaaac
     2821 agccagctgg cagcgaacaa gacgaggact acgacattgc ccaccccgtg agcaagtccg
     2881 acggattttg cacgcgtgcc cgacgggatg gcaaaccaat cgatccaccc cggaacggcg
     2941 gctatcagcc cgccgacgat tccggcggcg atcagccaga aggcgatgac cgaccactgc
     3001 tccgtgccgg tcgcgagata aaccacatca aaaacaactg ccgttgccag cagtcctaat
     3061 ggaaacacaa tcaaaatagg atgcgccgca tgtcccaaaa ctttagctct gctttccata
     3121 atctctttct cctgttcaaa tttatgtgcc gtaattcaat aggcttttcc aatcggtaat
     3181 taaagcctct atcctgtttg tttcagtaat cataagtttt tatttttgta aaaacattcg
     3241 ttaaaaaaca caataaacat attttgttta tcttattaat ttcgaatgga ttttcaagtc
     3301 gatttccgac cttttcaagc tgtaaattat tcgtctcgca aatcggatga agcggaatcg
     3361 ctctcgaaac gccttttccg gcgggagttt taaaatcttg ccgaaacctt gccggatttg
     3421 tttcgtaaaa taaattcgaa aacctgcatt ccggagttct ttaatccaag tcaaaaagga
     3481 atttataaac gaatgaaaat atttaccgca attctatttt tctgtcttac agctattgtc
     3541 gccgacgtcg ccggacaagc tcaaatgcct aacgcgacgc tgtttgcgcc cggtattgtt
     3601 tcaaccggat ttatggaaac gagcgcgact tttacgcccg acgggaagaa agtttatttt
     3661 acgcgctcgg acgttcaatt cagcgataac acgattatgc aatcgacctt taaaaacggt
     3721 cgctggagcg aggcgcaagt cgcttcgttt tcaggcgtct ggcgcgattc cgaaccgt
//
LOCUS       DASWNE010000288         3966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_9602, whole genome shotgun sequence.
ACCESSION   DASWNE010000288 DASWNE010000000
VERSION     DASWNE010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9602"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /locus_tag="VGB68_05905"
     CDS             complement(<1..1084)
                     /locus_tag="VGB68_05905"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY0048796.1"
                     /translation="MKVTNIQTSVTRDAVTDEDGFYRVGALDPGTYTVVIQKTGFSRA
                     ENRAVVVTTSQETTFDAELKPGSVTESVDITSDAEAVTLNKTNGTIAATFGERQVEEL
                     PLGAGRNVNNLALLSPNVFSAPGSTGISANGQRARNNNFTIDGSDNNDISVTIPTVGV
                     VPEGISEFQIQTNPYSAESGRNTGASINVLTKSGTNEFHGDLFEYYRGSDLNALTNVQ
                     KRQGVTEPPRFVRNQFGFAVGGPVYFMNVGDDGPSIYDGRNKTFFFYLFQRDPQRTGA
                     LTGATIRIPTPAGFAALNNVPLRAASGTTPAQSLASRQAVLQQLGFLQQIYAQNPTFT
                     NLVNITANGVPIQTGQTSIGIAQPSDT"
     gene            complement(1451..2860)
                     /locus_tag="VGB68_05910"
     CDS             complement(1451..2860)
                     /locus_tag="VGB68_05910"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0048797.1"
                     /translation="MAKKGSILVVDDEEIMRDVLETILSGAGYKVDLAKTGEEALEAY
                     GRKPYDVVLMDVSMPGIGGLTALEELVKIDDEAVVLMITAYATFDTAISAWEKGATGI
                     IRKPFQNEQILAMIGRGVKKRRHEEERQTLRQAMSRSVKREGFIGRSDIMENVFRMVD
                     RVAPARSTVLITGESGTGKELVAKAIHEASPRNNQPFVVVNCSNIPSDLLESELFGHT
                     KGAFTGAVGAKKGLFDVADGGSIFLDEIGDLRFETQVRLLRVIQEREFTPLGDTQPRK
                     VDVRIIAATNVDLKEAVRNGTFREDLYYRLSVVPMELPPLRDRREDILPLVVHFIRKY
                     NEENGRHISEQILPEVLSLLENYYYPGNVRELENIIERAVVIAPTDEITVECLRPEVR
                     NPEMAREMMRDSEGFSSDINISRGVNFYDEVRKFEVDLIRRALEQTGGHQSRAARLLG
                     LNATTLNSKIKTYNIPARA"
     gene            2989..3573
                     /locus_tag="VGB68_05915"
     CDS             2989..3573
                     /locus_tag="VGB68_05915"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0048798.1"
                     /translation="MIYDKLAKNYDKVFAPFEKRFVSEWRRETLSHLPADSRILEIGA
                     GTGLNFKFYPPCLQAVASEISFKMLEFAGQKTKTIKLVQANAERLPFAANTFDAAFAT
                     LVFCSIPKPENAFAELRRIVKPKGKIVLLEHVRPSNPLGAAFDFLNVFTVALIEDHFN
                     RRTAEIAEKAGLRILEVKRKAFGIINLIICEVVK"
     gene            3618..>3966
                     /locus_tag="VGB68_05920"
     CDS             3618..>3966
                     /locus_tag="VGB68_05920"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEY0048799.1"
                     /translation="MNTKKNKPKIQIGCQGWNYADWTTKPAGERIFYPRGTKSNEMLE
                     VYARAFESIEVDSTFYAIPASSAVENWFKKTPENFSFALKLPREITHDRSLNEKSFPA
                     LEEFCERILLLREK"
BASE COUNT          981 a         1054 c          991 g          940 t
ORIGIN      
        1 ccgtgtcaga aggctgcgcg ataccgatgc tggtttgtcc ggtctgaatc ggaacgccgt
       61 tcgccgtaat gttcactaaa tttgtgaacg tcgggttttg cgcataaatc tgttgcaaaa
      121 acccaagctg ctgcaatacc gcttgacgac tggcaagcga ttgcgccggt gtggttcccg
      181 atgcggcgcg cagcggaacg ttgttcagcg ccgcaaaacc agccggagtc gggatgcgaa
      241 tcgtcgcgcc tgtaagcgct ccggtgcgct gcgggtcgcg ctgaaacaga tagaagaaaa
      301 aagtcttgtt gcgtccgtca taaatgctcg gaccgtcatc gccgacattc atgaaataaa
      361 ccggtccgcc gacggcaaag ccgaattggt tgcgaacaaa acgcggaggc tcggtcacgc
      421 cctgcctttt ctgaacgttc gtcagcgcat tcaaatcgct tccgcgataa tattcaaata
      481 aatcgccgtg aaattcattg gttcccgatt tagtcagaac attgattgac gcgccggtat
      541 tgcgcccgct ttcggcgctg tacgggttag tctgaatctg gaattcggaa ataccttcgg
      601 gaacgacgcc gaccgtcggg atagtcacgc tgatatcgtt attgtccgaa ccatcaatcg
      661 taaagttgtt gttgcgggct cgctgtccgt tcgccgaaat gccggtcgag ccgggcgcgc
      721 tgaagacatt gggcgagagc agagctaaat tgttgacgtt gcgtcccgcg cccagaggca
      781 gttcttcgac ctggcgctcg ccgaaagtgg cggcgattgt gccattggtt ttgttcagcg
      841 tgaccgcctc ggcatccgac gtgatatcga ccgattcggt gacgctgccg ggtttcagct
      901 ccgcgtcaaa agtcgtttcc tgcgacgtcg taacgacgac cgcgcggttt tcggcgcgcg
      961 aaaaacccgt tttctgaatc acgacggtat acgtgccggg gtctaacgcg ccgactcgat
     1021 aaaatccatc ttcatcggtg acggcatcgc gcgtcacgct cgtttgaata tttgtaactt
     1081 tcacctcagc cccggcaaca atagcaccgg actggtcacg aacggtgccc gtgatagctc
     1141 cacgggttaa ttgcgcataa gcggaagttg aaagcaacaa acagaaaatg ctcactgtca
     1201 ttaaagcagt gacagtatta aacatccttt tcatactaag gctcctcttt atatttaata
     1261 atatggcgga tgttatgaaa ttaggtcagt attactaaaa acgcgttaag gcaatcttta
     1321 taccaaaaaa attgaaacgg cgagaaaatc atataaagac acttaattaa caatttaccc
     1381 gctaaagaaa cttttagcgg gtaaccaaaa aatacaaatt cttgaatatc aattcagaaa
     1441 ggtgcaaaaa tcaggcgcgc gccggaatgt tataggtctt gattttcgag ttcagcgtcg
     1501 tcgcgttcaa acccagcagg cgcgcggcgc gcgattgatg accgcccgtc tgttcgagcg
     1561 cgcggcggat taaatcaacc tcgaattttc gcacctcgtc ataaaaattt acgccgcgcg
     1621 aaatgttgat gtccgacgaa aatccttcgc tgtcgcgcat catctcgcgc gccatttccg
     1681 gattgcgcac ttccggacgc agacattcga ccgtgatttc gtcggtcggc gcgatgacga
     1741 cggcgcgctc gataatattt tccagctcgc ggacgtttcc cggataataa taattttcta
     1801 aaagcgacaa gacttccggc aaaatttgtt cggaaatgtg acgcccgttt tcctcgttgt
     1861 atttgcggat aaaatgcacg acgagcggca gaatatcttc gcgccggtcg cgcagcggcg
     1921 gcagttccat cggcacgacc gacagacggt aatacaaatc ttcgcggaaa gttccgttgc
     1981 gcaccgcttc ttttaaatcg acattcgtgg cggcgataat tctgacgtcg accttgcgcg
     2041 gctgcgtgtc gccgagcggc gtaaactcgc gctcctgaat cacgcgcagc agccgaacct
     2101 gcgtttcaaa acgtaaatcg ccgatttcgt cgagaaatat cgagccgccg tccgccacgt
     2161 caaacaaacc ttttttcgcg ccgaccgcgc cggtaaatgc gcctttcgtg tgaccgaaaa
     2221 gctcgctttc gagcaggtcc gaaggaatat tcgagcagtt gacgacgaca aacggctgat
     2281 tgttgcgcgg cgaggcttcg tgaatcgctt tggcgaccag ttcttttccg gtgcccgatt
     2341 cgcccgtaat caaaacggtc gagcgcgccg gagcgacgcg gtcaaccatc cggaaaacgt
     2401 tctccattat gtccgagcga ccgataaagc cctcgcgctt gaccgagcgc gacatcgcct
     2461 gccggagcgt ctggcgttct tcttcgtgac ggcgtttttt tacgccgcgc ccgatcatcg
     2521 ccagaatttg ctcgttctgg aaaggtttgc ggatgattcc cgtcgcacct ttttcccagg
     2581 cggaaatcgc cgtatcgaaa gtcgcgtaag ccgtaatcat cagcaccacg gcttcgtcgt
     2641 cgattttgac cagctcttcg agagccgtca aaccgccgat gcccggcatc gaaacgtcca
     2701 tcaaaacgac atcgtaaggc ttgcgcccgt aggcttcgag cgcttcttcg cctgttttcg
     2761 ccaaatcgac tttgtaaccc gcgccggaca aaatcgtctc cagcacatcg cgcataattt
     2821 cctcgtcgtc gacgacgaga atcgaacctt ttttcgccat aattttaaga agacagaaga
     2881 cagaataccg aaagtcggaa gtttttaaaa actttgccgt cgaatcaagt catcgaaccg
     2941 gaaacttttt tccgtcttcc gatttgcggt attctgtctt ctaaataaat gatttacgac
     3001 aaactcgcca aaaattacga taaagtcttc gcgccgtttg aaaaacgatt tgtctccgaa
     3061 tggcgcagag aaactttgtc gcacttgccc gccgactcgc gcattctcga aatcggcgcg
     3121 ggaaccggct tgaattttaa attctacccg ccttgccttc aagccgtcgc cagcgaaatc
     3181 tcattcaaaa tgctagaatt tgccggtcag aaaacaaaga ctataaagct tgttcaagcc
     3241 aacgccgaac gcctgccgtt tgccgcaaac acttttgatg cagcttttgc cacgctcgtt
     3301 ttctgctcga ttccgaaacc cgaaaacgct tttgccgagc tgcggcgaat cgtcaagcca
     3361 aaggggaaaa tcgttctgct ggaacacgtg cgcccgagca atccgctggg cgccgcgttt
     3421 gattttctaa atgtttttac cgtcgcctta atcgaagacc atttcaatcg ccggacggct
     3481 gaaattgccg aaaaggcggg cttaagaatt ctggaagtaa agcgaaaggc gtttggaatt
     3541 atcaatctga ttatttgcga agtggtaaaa tagttgcgga aagttaacca aagataaatg
     3601 cggatttgtt atcttcaatg aacacaaaaa agaataaacc gaaaatccaa atcggctgcc
     3661 agggctggaa ctacgccgac tggacgacca agcccgccgg cgagcggatt ttttacccgc
     3721 gcggaacgaa gtcgaatgag atgctcgaag tttacgcgcg cgccttcgaa tcaatcgaag
     3781 tcgattcgac attttatgcg attccggcaa gctcagccgt cgaaaactgg tttaagaaaa
     3841 cgccggaaaa tttcagtttc gcgttgaagc tgccgcgcga aatcacgcac gaccgcagcc
     3901 tgaacgaaaa atcttttccg gcgctcgaag aattttgcga acgcatttta ttgctcaggg
     3961 aaaagc
//
LOCUS       DASWNE010000289         3081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_257220, whole genome shotgun sequence.
ACCESSION   DASWNE010000289 DASWNE010000000
VERSION     DASWNE010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257220"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VGB68_05925"
     CDS             <1..251
                     /locus_tag="VGB68_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY0048800.1"
                     /translation="VTCSGQQVKLTRKEFALLTNLAKNAGRVATRQNLLDNVWGYSYF
                     GDTRTLDVHIRRLRQKLGDCADCIETVVGVGYRFIGMK"
     gene            379..1464
                     /locus_tag="VGB68_05930"
     CDS             379..1464
                     /locus_tag="VGB68_05930"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0048801.1"
                     /translation="MKNFLGNWFTKASEAQKSPRRILIDILKTMREGVIIVADDTRII
                     ASNQAAYDAFARNNGALETKRLSEIIRDVSVHEAFRRALVEQQSSEINFEILAGEKRS
                     FDVHIAPVEIEGEPSAVGIFYDTTQIERLETIRQEFLSNVSHELRTPLTSILAFVETL
                     EDGAIEDHENNLRFLGVIRRNAERMHRLIDDILELSSIEAGKIDVEAKQVNLSKMIEE
                     VFTNLAGKAREKKIKLKNEVAADVLVFADIVRLEQMLTNLVDNAVKFNREAGSVKISH
                     RRNETHDLISVEDMGEGISNEHLGRIFERFYRTDKARSREIGGTGLGLAIVKHLARLH
                     GGEVSVASTVGKGSVFTIELLRSEKTA"
     gene            1471..1632
                     /locus_tag="VGB68_05935"
     CDS             1471..1632
                     /locus_tag="VGB68_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048802.1"
                     /translation="MPPEAVFKLRNCYSPANPGAKASSAEIPVDVPDSNEAYKKRLDS
                     GSEVEPFFI"
     gene            complement(1654..>3081)
                     /locus_tag="VGB68_05940"
     CDS             complement(1654..>3081)
                     /locus_tag="VGB68_05940"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEY0048803.1"
                     /translation="FNPIINGFEIRAIIAESDGRIVIGGIFSGINGFSRSNLARLGAD
                     GSLDAAFNAGSIGTVYKIVRQADGKYLILSGGVSRRSNDGSVDNTFQPPTILSGDSLP
                     VKDFALQPDGTIIIGGVFNTVSNFTRNNIARLLPDGALDRFYLFGGTNAGVKALLSQP
                     DGKTVVGGSFTIIENTSRSGLARINNAPIVIRVPYFDFNGDGKSDISVFRPSNNFWYI
                     ARPTGAPAQNFDATQFGASGDIIVPADYDGDGRADVAVFRPSDGVWYLMQSSAGFRAA
                     QFGAGGDIPVPGDYDGDGKANLAVFRPSTRSWYIARAGGVPSQNFDSVPFGNSTDKPV
                     SGGDFDGDGKTDVTVFRPSNGYWYRLNSSNGHFIAVQFGIAEDKPVAADYDGDGKTDI
                     AVFRPSDGVWYRIHSGTNSFTATQFGIAEDRPAPGDYDGDGKADFAVFRPSSGIWYLQ
                     QSSAGFAAAQFGAGGDLPAPNAFVR"
BASE COUNT          726 a          860 c          854 g          641 t
ORIGIN      
        1 gcgtcacgtg cagcggtcag caggtaaagc tgacgcgcaa ggaatttgcc cttttgacca
       61 atctggcgaa aaatgccgga cgcgtcgcga cgcgtcagaa tctgctcgat aacgtctggg
      121 gctacagcta cttcggcgat acgcggacat tggacgtcca tattcgccgg ctgcgccaaa
      181 agctcggcga ctgcgccgac tgcatcgaaa cggtcgtcgg cgtcggctat cgctttatcg
      241 ggatgaaata gagctatcag ccatcagcgt tcagccgtca gctgcctact gatgcttact
      301 ggttgaatga agccgggtat cgcaggcaag gcaaatagct gtctgccgac ggctaaacgc
      361 tgacggctat ttactattgt gaaaaatttt ctcggcaatt ggtttacaaa agcttccgaa
      421 gcgcagaaat ctccgcgccg gattttgatt gatattctca aaacgatgcg cgagggcgtg
      481 attatcgtcg ccgacgatac gcgcattatc gcctcgaatc aagccgccta cgacgctttc
      541 gcccgaaata acggcgcgct cgaaaccaaa cgattgtcgg aaattatacg cgacgtttcc
      601 gtccacgaag cgtttcgccg cgcgctcgtc gaacagcagt cttcggaaat aaattttgag
      661 attttagcgg gcgagaagcg ctctttcgac gttcacatcg cgccggtcga gattgaaggc
      721 gagccgagcg ccgtcgggat tttttacgac acgacgcaaa tcgagcgtct ggaaacgatt
      781 cggcaggagt ttctgtcaaa cgtctcgcac gagctgcgca cgcctctgac ttcaatcctc
      841 gcttttgtcg aaacgctgga agacggcgcc atcgaagacc acgaaaacaa cctgcgtttt
      901 ctgggcgtga ttcgcagaaa cgccgagcga atgcaccgtt tgattgacga tatcctggag
      961 ctttcgtcta tcgaagcggg aaaaatcgac gtcgaggcga aacaggtcaa tctctcgaaa
     1021 atgatagagg aagttttcac caatctcgcc ggaaaagcgc gcgaaaaaaa aatcaaattg
     1081 aaaaacgaag tcgccgccga cgtgctggtt ttcgcagaca tcgtccgcct ggaacagatg
     1141 ctgacgaatc tggtcgataa cgccgtcaag tttaaccgcg aagcgggaag cgtgaaaatc
     1201 tcgcaccggc gaaacgaaac tcacgatttg atttcggtcg aagatatggg cgaaggcatc
     1261 tcaaacgagc atctgggacg cattttcgag cgtttttacc ggacggataa agcgcgctcg
     1321 cgcgaaatcg gcgggacggg cttgggcttg gcaatcgtca aacatctggc gcgactgcac
     1381 ggcggcgaag tttccgtcgc ttcgaccgtc ggcaaaggct cggtttttac aattgagctg
     1441 ttgcgctctg aaaaaacggc ttgacccgca atgccgcccg aagccgtctt caaattaaga
     1501 aattgttatt cgccggctaa tccgggcgca aaagcatcgt cggcggaaat accggtcgat
     1561 gttccagact cgaacgaggc ttataaaaaa cggctcgact ctggttccga agtcgagccg
     1621 tttttcattt aagagcttta gcccgataac taatcagcga acaaaagcgt tcggcgcggg
     1681 caaatcgccg ccggcgccga attgcgcggc ggcaaatccc gcgctcgact gctgcagata
     1741 ccagattccg ctcgacgggc gaaacaccgc gaaatcagcc ttcccgtcgc cgtcgtaatc
     1801 gccgggcgcg ggtcgatctt cggcgatgcc gaactgcgtt gccgtgaaag aattagtgcc
     1861 gctgtggatg cggtaccaaa ctccatccga cggacggaag acggcaatat cggttttgcc
     1921 gtcgccgtcg taatcagccg ccacgggctt gtcttcggcg atgccgaatt gaacggcaat
     1981 aaagtgcccg ttcgagctgt tcagacgata ccagtatccg ttcgacggtc tgaaaacggt
     2041 cacatccgtc ttgccgtcgc cgtcgaaatc tccgcctgaa accggcttat ccgtgctgtt
     2101 gccgaaaggc acggaatcga aattctgcga cggaacgccg cccgctctgg cgatatacca
     2161 ggaacgtgtc gatggacgaa agacggctaa attcgctttg ccgtcgccat cgtaatcacc
     2221 cggaacggga atatcgccgc cggctccgaa ctgcgccgct ctgaatccgg cgctcgactg
     2281 catcaggtac caaacgccgt ccgaagggcg aaaaaccgcc acatcggcgc gcccgtcgcc
     2341 gtcgtaatcc gccggaacga ttatgtcgcc gctcgcgccg aactgcgtcg cgtcgaaatt
     2401 ctgtgccgga gcgccggtcg gacgcgcgat ataccagaaa ttgtttgacg ggcgaaaaac
     2461 cgaaatatcc gatttcccgt cgccgttaaa atcgaaatac ggaactctga tgacgatagg
     2521 cgcgttattg atgcgcgcca atccggaacg actggtgttt tcgataatcg taaagcttcc
     2581 gccgacgacg gtttttccgt ccggctggct gagaagcgct ttgactccgg cattcgtgcc
     2641 gccgaacaga taaaatctgt ccaaagcgcc gtccggcaaa agccgcgcga tattattgcg
     2701 agtgaaattg ctgaccgtat tgaacactcc gccgatgata atcgtgccgt ccggctgaag
     2761 cgcgaaatct ttgaccggca gcgagtcgcc gctgagaata gtcggcggct ggaaagtatt
     2821 atcgacgctg ccgtcgttgc tgcgccgcga gactccgccg ctcagaatca gatatttccc
     2881 gtccgcctgc cggacaattt tatagacggt gccgatgctg ccggcgttaa acgccgcgtc
     2941 gagcgagccg tccgcgccga gccgcgccag attcgaccgg gaaaagccgt taatgccgct
     3001 aaagatgccg ccgataacga ttctgccgtc gctttcggcg atgattgcgc gaatctcgaa
     3061 gccgtttata atcgggttaa a
//
LOCUS       DASWNE010000290         3075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_433583, whole genome shotgun sequence.
ACCESSION   DASWNE010000290 DASWNE010000000
VERSION     DASWNE010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433583"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VGB68_05945"
     CDS             complement(<1..255)
                     /locus_tag="VGB68_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048804.1"
                     /translation="MKNLLQRLAQFEPTGFPFLNFYLNAQVNENGQRTFGVYARTQLS
                     ESAKNYAEESSERESFDRDAQKILEYLETVRPTAQGVAIFA"
     gene            complement(320..781)
                     /locus_tag="VGB68_05950"
     CDS             complement(320..781)
                     /locus_tag="VGB68_05950"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY0048805.1"
                     /translation="MNATELLKQDHREVDSLIAELEGASGDTAGSYTATFLKLKTSLT
                     LHAEAEEQIFYPAMEQFEETEEITEEAYSEHDEVKQLLEDMTGLNPGDEEFQELLAQL
                     KDSVQHHVEEEETEMFPKAEELLGDATLETMGDEIEALKGEASGTSRIAKA"
     gene            1109..1498
                     /locus_tag="VGB68_05955"
     CDS             1109..1498
                     /locus_tag="VGB68_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048806.1"
                     /translation="MSNQQNDPQYGADYHGNIKISGNEEMPSGSEEPNSKRDQQFVSA
                     SQAIKSSSGTGEGDEQSQPDIEPSETGSGHSGTENQSGDPGRTPGKAEGDDDSSAESQ
                     NNQQSEDVENYEQTKEKKIAESVSTSS"
     gene            1695..1910
                     /locus_tag="VGB68_05960"
     CDS             1695..1910
                     /locus_tag="VGB68_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048807.1"
                     /translation="MQNIPNPDVNSPDVDSTNQNNSDIEPSRSDKSDEAIPVPPDKQP
                     TVPIEDPPDVQNKPPIGEDQTEPQRIV"
     gene            2096..2362
                     /locus_tag="VGB68_05965"
     CDS             2096..2362
                     /locus_tag="VGB68_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048808.1"
                     /translation="MKLVGRRNPKRTGFRICLRIFIYEKQGKGTRARGSPDGNRFSLN
                     GKENQAADLTGAKRREEKVKMFPIILRLSFFGLDKLLNKSSAIL"
     gene            2522..>3075
                     /locus_tag="VGB68_05970"
     CDS             2522..>3075
                     /locus_tag="VGB68_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0048809.1"
                     /translation="MKLSLKKLNEQVIVITGATSGIGLTTARMAAEKGAKLVLVARNG
                     DALRELADELSANGTRVIYSIADVADETVLRRAAEIAKANFGGIDTWVNNAGGSVYGR
                     IMDVPTEDLRRLFETNVWGVVNGSKIAVEHLRERGGALINIGSEVSDAPVPLQGMYSA
                     SKHAVKGFTNALRMELEADELPISV"
BASE COUNT          845 a          703 c          789 g          738 t
ORIGIN      
        1 ggcaaaaatt gccacgccct gcgccgtcgg tcgaaccgtt tcgagatatt ccagaatttt
       61 ttgcgcgtcg cggtcgaagc tttcgcgctc ggaagattct tccgcgtaat tttttgcgct
      121 ttcgctgagc tgtgttctgg cgtagacgcc aaaagttctc tgcccgtttt cgttgacctg
      181 cgcgttcaga taaaaattta gaaacggaaa accggtcggc tcgaattggg cgagtctttg
      241 cagtaaattt ttcataggtt tttcctcaaa taaaaggcgc gtaaacattt agtttacgcg
      301 cccgtgtttt cgattaaact taagccttag cgatgcggct cgtgccggaa gcttctcctt
      361 tcagcgcttc gatttcgtcg cccatcgtct ccagcgttgc gtcgccgagc agttcttcgg
      421 ctttcggaaa catctcggtt tcctcttcct cgacgtgatg ctggacgctg tctttgagct
      481 gcgccagcaa ttcctgaaat tcttcgtcgc ccggatttaa acccgtcata tcttccagca
      541 attgcttgac ttcgtcgtgt tccgaatagg cttcttcggt tatctcctcg gtttcttcaa
      601 actgttccat cgccggataa aagatttgct cttcggcttc ggcgtgcagc gtcaaagaag
      661 ttttcagttt tagaaaagtt gcggtataag aaccggcggt atcgccgctc gcgccttcaa
      721 gctcggcaat caggctgtcc acctcgcgat ggtcttgttt taaaagctcc gttgcgttca
      781 tcgtgtttct ccttaaaaaa tataaaaaat ttcgtgtgtg aagccttgcc gactgcaaga
      841 gttgtgcctg ttttttttta aagcggcgga agtattaatt tcaataaaaa ggcgaattaa
      901 aaaaataatt ttgttatttg ataaaaagcg aatcggctaa ttgccttaat ttaatacgct
      961 ctttctgtat cctattcttt cactggcggc tgaaaaatcc ggcaaagctt tgtataacgg
     1021 gcagtttcgg caaatctgcg ggcgggaacg atttttgcaa acataaaaat ttctacatat
     1081 aaattataaa aaagtctaag gaggcagaat gagcaatcaa caaaacgacc cgcagtatgg
     1141 agccgattat cacggaaaca taaaaatcag cggcaatgaa gaaatgccga gcggcagcga
     1201 agagccgaat tcgaaaagag accagcagtt tgtctcggcg tcgcaggcga taaaatcgag
     1261 cagcggcacg ggcgaaggcg acgagcagag ccagccggac atcgagccga gcgaaacggg
     1321 cagcgggcat tcaggcacgg aaaatcaatc gggcgatccg ggacgcacgc ccggcaaagc
     1381 cgaaggcgat gacgacagct cggcggaatc gcaaaacaac cagcaaagcg aagacgtgga
     1441 aaattacgag cagaccaagg aaaagaaaat tgcggaatcg gtttccacca gcagctaaaa
     1501 gctgttagaa ggtaaagatt ttcgtctttg cgtccctggc gaaaaatttc gcgcttttcg
     1561 cgactaggaa atttttaacg ccagggacgc aaagtttaag acgagaaccg cgcaggatga
     1621 tttccgagcc agccggcgag aaaatctgac cggcagcatc aggcttaaat ttttgaaagg
     1681 aaatttcgta aattatgcag aatattccga acccggacgt aaattcgccg gacgtcgaca
     1741 gcacaaatca aaacaactcc gatattgagc cgtcgcgctc cgacaaaagc gacgaagcga
     1801 ttcccgttcc gccggacaag caaccgacgg ttccgataga agacccgccg gacgtgcaga
     1861 acaagccgcc aatcggcgaa gaccagaccg agccgcaacg aatcgtttaa aaaaccggct
     1921 aaaagaagcc acagaccgaa gatttttatt tttgtctgtg gtttttttat tgcatcgggc
     1981 gcgcgagatt atttgtagct atcggataca acgcgtagcg gattcgtatt aataagatac
     2041 gaatctttcc tgattagcgt ctggcatcag tcttgcacga ctttgaattc ttactttgaa
     2101 actcgtcgga aggagaaatc caaagcgtac cggttttcgg atatgcctaa gaatatttat
     2161 ttatgaaaag cagggcaaag gtactcgggc acgcggctca ccggatggta atcgtttttc
     2221 tttaaatgga aaagaaaatc aagccgcaga tttaaccggc gcgaaacggc gcgaagaaaa
     2281 agtaaagatg tttccgatta ttttgcggct gagctttttc gggttggata aacttttaaa
     2341 taagtcttcc gcgattttat aaacgggtga gccgaaatat aaaagttttc agcgccggga
     2401 cgcggaggca aaagacaaaa ggtaaaagta aaaataatct tgtcatttta cttttcactt
     2461 taaattcccg gcggtgaaaa acagttagat tataaaaatt tgagcaaagg ggaaatctgt
     2521 aatgaaacta agtctgaaaa aattaaacga acaggtcata gtgataacgg gcgcaaccag
     2581 cggcatcggc ttgacgacgg cgcgaatggc ggctgaaaaa ggcgcgaaat tagttctcgt
     2641 ggcgcgcaac ggcgatgccc tgcgcgagct ggcggacgaa ttgagcgcca acggcacgcg
     2701 cgtaatttat tcgattgccg atgtcgccga cgaaacggtt ctgagacgcg cggctgaaat
     2761 tgcgaaagcg aatttcggag gcatcgacac ctgggtaaac aacgcgggcg gctcggtata
     2821 cgggcggata atggacgtgc cgaccgaaga tttgcgccgc ttgtttgaaa cgaatgtctg
     2881 gggcgtagtc aacggctcga aaatcgccgt cgaacatttg cgcgagcgcg gcggcgcgct
     2941 gattaatatc ggcagcgaag tttccgacgc gcccgtgccg ctgcagggaa tgtattcggc
     3001 gtcgaaacac gcggtcaaag gttttacgaa cgctctgcgg atggagctcg aagccgacga
     3061 attgccgatt tcggt
//
LOCUS       DASWNE010000291         4289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_433629, whole genome shotgun sequence.
ACCESSION   DASWNE010000291 DASWNE010000000
VERSION     DASWNE010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433629"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VGB68_05975"
     CDS             complement(<1..252)
                     /locus_tag="VGB68_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048810.1"
                     /translation="MKQGFLRIFVLSAMGLLLIAADASAQQTERERRAANAAGSIYVI
                     SAKAGGVNFVEGKVAIARKDAKSGYLIKGDELEIGDKVST"
     gene            452..709
                     /locus_tag="VGB68_05980"
     CDS             452..709
                     /locus_tag="VGB68_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048811.1"
                     /translation="MATFKDPIKCFAPLEVVERLLEMRAELGYTEAIYLNSSLELADF
                     LDAFGFDVYAFSTKDEKINYGDYRSSLVPGGDALHLSLTAH"
     gene            complement(906..1316)
                     /locus_tag="VGB68_05985"
     CDS             complement(906..1316)
                     /locus_tag="VGB68_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048812.1"
                     /translation="MDVYHRVLTRLYEVTDGKDSKAVDLKDLVKSLGFLGNYPDIFER
                     LSAAGWIAEDKKANFVRITHWGVSEVKKSPAAANQSNNNLKSDADKAIAEAKELGLLL
                     EIFASDASKDNLTPVERKIDNLKAVIDRIKSNLG"
     gene            complement(1443..2405)
                     /locus_tag="VGB68_05990"
     CDS             complement(1443..2405)
                     /locus_tag="VGB68_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY0048813.1"
                     /translation="MEIVLACDLGGTNLRMAAVDEQGNIHHRLKVETPKTERAGEIVR
                     VTVEAANKCAAAVKKIGEVKALAAAAPATINTEKGLILKAPNVPALDGCRFAAVLSNE
                     LNLPCVLENDANAAVIGEHAFGAAKGFRSAIAVTLGTGVGGGIIIDNKILRGADGTAG
                     EIGHICVEPFGVPCGCGSRGCVEQYSSATAIVRLAQELENQYPKSVLRSRSHLTSADI
                     YKAGKAGDDLALEVFRQMGFYLGIALAGLINVLNPEVIVIGGGAGAGWDLFVPHTIEQ
                     IRQRAYREPAERAKLVKAELGDDAGILGAAQLAFKAVNKTQARV"
     gene            2666..4189
                     /locus_tag="VGB68_05995"
     CDS             2666..4189
                     /locus_tag="VGB68_05995"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0048814.1"
                     /translation="MAAATAEQAGNYQNAISKSKIWKVIAAASIGTMIEWYDFYIFGS
                     LALTISPLFYPPGDNTFAYIAYLATFAVGFVVRPFGALFFGRIGDIVGRKYAFLVTLL
                     IMGGATAIIGFLPTFSQIGYAAPIILLLIRILQGLALGGEYGGAAVYVAEHVPDNKRG
                     FYTSFIQITATLGLFLSLAVILSVQNMMSAEQFAGKTESFAGWRIPFLISIFLVGVSL
                     YIRLRMKESPIFQHVKSAGMTSANPLIEAFTKWENLKRVLISLFGATAGQGVVWYTGQ
                     FYALFYLQSILNVNQTSANYIIATALLLGMPFFVIFGALSDRIGRKWIIMAGLLLAVI
                     TYIPIYQAMQSAAGSNVVTASSKRDQITGAINLIPQSSDANGNLTASPKVLPYTNFGD
                     LISNPAAWKLILLVFIQVIWVTMVYGPIAAYLVEAFPAKIRYTALSLPYHIGNGVFGG
                     LLPVIGLSIIAETGNIYAGLYYPMIVAAITFLIGSFLLKETRGHRIWEEVESETARK"
BASE COUNT          970 a         1095 c         1035 g         1189 t
ORIGIN      
        1 ggtcgaaacc ttgtcgccga tttccagttc gtcgcctttg attagataac cgcttttcgc
       61 gtcttttcgc gcgatcgcga ccttgccctc gacaaaatta actccgcccg ctttggcgga
      121 aataacgtaa atgctgccgg cggcgttggc ggcgcgtcgc tcgcgctcgg tctgctgagc
      181 cgacgcgtcg gcggcaatta acagcaagcc catcgcgctt aatacaaaaa ttcttaaaaa
      241 gccctgtttc attagtttta ctccttgtta tccggacatt tgagagtgtg gttcttataa
      301 tagcgtgttt ttgctaatta ttccaataaa tttgcggcaa aaaagttatt cgcggcgggc
      361 taaaaattgg aacaaccttt gattgtcttc cgtaatcaaa gaaagtgcct tacagaaatt
      421 taaggcaaat tgatagttaa ggaaatcgaa aatggctacg ttcaaagacc cgattaaatg
      481 ttttgcgccg ctcgaagttg tcgagagatt gctggaaatg cgcgcggagc tcggctacac
      541 ggaagccatc tatttaaaca gctcgcttga gctggcggat tttctggacg ctttcggttt
      601 tgacgtttac gccttttcga caaaagacga aaaaattaat tacggcgatt atcgctcgtc
      661 gctcgtgccg ggcggcgatg ctttgcattt atcgctgacg gcgcattaac acaaaatccc
      721 aaatgacaac tgaataggta ggtaagaaga agaggaagga gaagcaaaat tctccttcct
      781 cttttgtttt tgacgaagca aaaatgtatg gacgcccgcc gcgacgcgtc catacattta
      841 tttttccgtt tatttttccg tttatttgtc cgttactcga tttgattttg aaatatccgc
      901 aacggctaac cgagattcga ttttatccgg tcgatgacgg ctttcaggtt atcaattttc
      961 cgctccaccg gcgtcagatt atcttttgaa gcatcactgg caaaaatttc cagcaataaa
     1021 cccagctctt tggcttccgc tatcgccttg tcggcgtcgc ttttcagatt gttgttcgat
     1081 tgatttgccg cagccggaga ttttttaact tcggaaacgc cccagtgcgt gatgcgtacg
     1141 aaattcgctt ttttgtcttc ggcaatccag cccgcggcgc tcaagcgttc aaaaatgtcc
     1201 ggataatttc ccagaaagcc gaggcttttg accaaatcct tcaaatcgac ggctttcgag
     1261 tcttttccgt cggttacttc ataaagcctt gttaaaactc gatgatatac gtccataatt
     1321 ttagtccaaa attaatcggg cgcggcggcg ccggaaaggc aagaagccac agattaacac
     1381 agatttgacg gaattttgtt tttttatttg cgctctgccg aaacgctttc ttcaattacg
     1441 aattacactc gcgcctgcgt cttattcact gctttaaacg ccagctgcgc cgcgcccagt
     1501 atgcccgcgt cgtcgccaag ctcggctttt acaagctttg cgcgttcggc aggctcgcgg
     1561 taagcccgct ggcgaatctg ctcaatcgtg tgcggaacaa acaaatccca gcccgcgccg
     1621 gcgccgccgc cgataactat aacttccgga ttcaaaacat ttatcagacc cgccagagcg
     1681 attccgagat aaaatcccat ctgccggaag acttcgagcg cgaggtcgtc gcctgctttt
     1741 cccgctttat aaatatcggc tgaggtcaga tgcgagcggc ttcgcaaaac ggatttcgga
     1801 tactgatttt ccagctcctg cgccaaacgc acgattgccg tcgccgacga atactgctcg
     1861 acgcagccgc gactgccgca gccgcacggg acgccgaaag gctcgacgca aatatgtccg
     1921 atttctccgg ctgttccgtc ggcgccgcgc aaaattttat tgtcgatgat aatgccgccg
     1981 ccgacgcccg ttccgagcgt cacggcgata gccgacctga aacctttcgc cgcgccgaaa
     2041 gcgtgctcgc cgataacggc ggcgttcgcg tcattttcca gaacgcacgg cagattcagc
     2101 tcattggaaa gcaccgcggc aaaacgacag ccgtcgagcg ccggcacgtt tggagctttt
     2161 aaaatcaagc ctttctcagt gttgatggtt gccggcgctg ccgccgccag agcttttact
     2221 tcgccgattt ttttgaccgc ggcggcacat ttattggcgg cttcgactgt gacgcggacg
     2281 atttcgccgg cgcgttccgt cttcggcgtt tccactttca gacgatgatg aatgtttccc
     2341 tgctcatcga ccgccgccat tcgcaggttg gttccgccca aatcacaggc taagacaatt
     2401 tccataattt cgtgccaatc aaaattccca taagtccggc tgcgcccgtc aataatattt
     2461 tatagcaata aagcttttgt tttatttcgt tttcgctcgt tttgacaagc tcggactttg
     2521 gactttagac tttgggctta aatagtttaa ctatctcgcg cgaatcttcc aaacaggata
     2581 caactcgaat gacttttggt tgtcgtcgat aaagaacaaa tgcgcggctt tttgcagaaa
     2641 attttaatca atcgaggaaa ttaaaatggc ggcagcgaca gcggaacaag ccgggaatta
     2701 ccagaacgct atttcaaaaa gtaaaatctg gaaagtcatt gcggcagcat caatcgggac
     2761 gatgattgaa tggtacgact tttatatttt cggcagtctg gcgctgacta tttcgccgct
     2821 gttttacccg ccgggcgaca atacgtttgc gtatatcgct tatctggcga cgtttgccgt
     2881 cggtttcgtc gtgcgcccgt tcggcgcttt gtttttcggt cgcatcggcg acattgtcgg
     2941 gcgcaaatac gcttttctcg tgacgctttt gattatgggc ggcgctacgg caattatcgg
     3001 ttttctgccg actttttcgc aaatcggcta tgccgcgccg attattttac tgctgattcg
     3061 cattttgcag ggtctggcgc tcggcggcga atacggcggc gcggcggttt acgtcgccga
     3121 gcacgtgccg gacaataaac gcggtttcta cacgtcgttt atacagatta cggcgacgct
     3181 cggtttattt ctctcgctcg ccgtcattct ttccgtccag aatatgatga gcgccgagca
     3241 atttgcggga aaaaccgaaa gctttgccgg ttggcgcata ccgtttttga tttcgatttt
     3301 tctcgtcggc gtctcgcttt acattcgcct gcgaatgaag gaatcgccga tttttcagca
     3361 cgtgaaaagc gccggaatga catcggctaa tcctttaatc gaagctttta caaagtggga
     3421 aaatctgaaa cgagttttaa tctcgctgtt cggcgctaca gccgggcagg gcgtcgtctg
     3481 gtataccgga caattttacg ccctgttcta cctgcaatct atccttaatg ttaatcagac
     3541 ttccgccaat tatattatcg cgacggcgct gcttttaggt atgccgtttt tcgtaatctt
     3601 cggcgctttg agcgaccgta tcgggcggaa atggattatt atggctggct tgctgctggc
     3661 ggttattact tatattccga tttatcaggc gatgcagtcg gctgctggtt caaacgtcgt
     3721 gacggcgtcg tcgaaacgcg accagataac gggcgcaatt aacctgatac cccaatcatc
     3781 agacgcaaac ggcaatctga ccgcttcgcc gaaagttctg ccgtacacga atttcggcga
     3841 ccttatttcc aatcctgccg cctggaaatt gattctactg gtatttatac aggtcatctg
     3901 ggtgacgatg gtttacggtc cgattgccgc ttatctggtc gaggcttttc ccgcaaaaat
     3961 tcgttatacc gccttgtcgc tgccgtatca tatcggcaac ggcgtgttcg gcgggctgct
     4021 gccggttatc ggtttgtcga taatcgccga gacgggcaat atctacgccg gtctttatta
     4081 cccgatgatt gtcgccgcca ttacattttt aatcggctcg tttttgctaa aggaaacgcg
     4141 cgggcatcgc atctgggaag aagttgaatc ggaaacggcg cgcaaataga ttttgacact
     4201 gccgagcgtt ttgttatcaa ataatttcag gtaaaaatta tgtccgaatc aacagccatc
     4261 gaatcgattc tgcacgaaga gcgcgtttt
//
LOCUS       DASWNE010000292         3443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_644873, whole genome shotgun sequence.
ACCESSION   DASWNE010000292 DASWNE010000000
VERSION     DASWNE010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644873"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VGB68_06000"
     CDS             complement(<1..510)
                     /locus_tag="VGB68_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048815.1"
                     /translation="MLIVMKPDAARSDVERVVEIIEKLGFKPHELPGESRTAIGVTGN
                     PGAIDPTHFENLPGVAEAIRVTKQYKLISRDLRPEKSVIKVGNAAVGDGNLAIIAGPC
                     AIETPEQVFKVAESVAKSGARFFRGGAFKPRTSPYAFQGKGEDGLKILAEVREQYGLN
                     IVTEAMDEHG"
     gene            complement(797..2800)
                     /gene="argS"
                     /locus_tag="VGB68_06005"
     CDS             complement(797..2800)
                     /gene="argS"
                     /locus_tag="VGB68_06005"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEY0048816.1"
                     /translation="MNLLQLQEILRGKVVETAGENFQIELEQPPMEVPPKTELGDLAF
                     PVAFELAKRIKQATGEKKNPREIAETLKSVLEKEDFVARVEVAGAGYLNVFYNRAGFL
                     ADASTAEPLSKLKKYEENAPKRMVEHTSVNPNKAAHIGHVRNAVLGDTFLRILQANGE
                     RTETQYYIDNTGVQVADVVVGFVHIKKMNLDDIKQLDEDLREEGKTFDYYCWDLYARV
                     GQEYQNDEELKAKRAETLHLIEEGGNEIAELADYVATRNVQCIVNTMERLSIRYDLLP
                     RESEILHLKFWAKAFDLMKQLGVIRLESEGKNAGCWVMPFEAHTGTDEHEADKILVRS
                     NGTVTYTGKDIAYQLWKLGVLGLDFNYKLFSTYFDGKEVWVTTAEAADHAAAPKFGSG
                     ETVYNVIDSRQSYPQEVVKKGVSLISPDKGERASIHLSYEMVALSPSAAEELGFRLAE
                     EDKQKSFIEMSGRKGLGVKADDLISRLEVNALQEVENRHPDISVAEKRDIAHTIAVGA
                     LRYFLLKFTRNTVIVFDFKEALSFEGETGCFCQYSAVRANSIFRKLEEFGLEDAVKTV
                     SEKRSEAAEILASENEVWSLITLASRLEETVAQAVAATEPAILAKYTFGLAKAFNLFY
                     HNHKIISEENEIKRAVLIVAADITRRALTAALNTLGIEVPERM"
     gene            complement(3158..>3443)
                     /locus_tag="VGB68_06010"
     CDS             complement(3158..>3443)
                     /locus_tag="VGB68_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048817.1"
                     /translation="SETGYADIRVRFARGRTSATMTGRIGTGGRICYVANAKAGQTLT
                     ATVSSRSGQVRIFESGDTSYQTEIEYSGDQSVCVDNLRGATTYSLTVSIR"
BASE COUNT          768 a          907 c          839 g          929 t
ORIGIN      
        1 gccgtgctcg tccatcgctt cggtgacgat attcaagccg tattgctcgc gcacttccgc
       61 taaaatcttc aagccgtcct cgcccttgcc ctggaaagcg taaggcgacg tgcgcggttt
      121 gaaagcgccg ccgcgaaaaa atctggcgcc gcttttcgcc accgattcgg cgaccttgaa
      181 aacctgttcg ggcgtttcaa tggcgcaagg tccggcgata atcgccagat tgccgtcgcc
      241 gacggcggcg ttgccgacct tgatgacgga tttttccggg cgcaaatcac gcgagattaa
      301 cttgtattgt ttggtgacgc ggatggcttc agccacgccc ggcagatttt caaaatgcgt
      361 cgggtcgata gcgcccggat tgccggtgac tccgatagcg gtgcggctct cgcccggcag
      421 ctcgtgcggc ttaaagccga gtttttcgat tatttcgacg acgcgctcga catcgctgcg
      481 agccgcgtcg ggtttcatga cgattaacat aaaattttct caaactgatt ttggcaaatt
      541 gcgagcgctt tcggcaaatt gccacgattt agccgcagat ttacgcggat aaacgcagat
      601 tttttaaata tcaagctccg attactatac agtgcaaatt tattgccgga aaatccggag
      661 caacaggctc taagatttta ccgcccgagc aaagattatg ataagcgaat aacgatactt
      721 aacataaatt tacaatttag aatttacaat tttcgctttc ttatttttga gcgaagcctt
      781 tttcactatt ttcgtcctac attctttccg gcacttcgat tcccagagta ttcaaagcgg
      841 cggtcaaggc tcgtctggta atatcagccg cgacaatcaa aacggcgcgt ttaatttcat
      901 tttcttccga gatgatttta tggttgtgat agaaaagatt aaacgctttt gcgagtccga
      961 acgtgtattt cgccagaatc gccggctcgg ttgcggcgac ggcttgcgcg accgtttctt
     1021 ccagccgcga ggcgagcgta atcagcgacc agacttcgtt ttcgctcgcc agaatctcag
     1081 ccgcttccga gcgtttttcc gagacggttt tcacggcgtc ttccaagccg aattcttcca
     1141 gctttctgaa aatcgaattg gcgcggacgg cggaatattg acaaaagcaa cccgtctcgc
     1201 cttcaaacga aagcgcttcc ttgaaatcga aaacgatgac ggtgttgcgc gtgaatttaa
     1261 gcagaaaata acgcagtgcg ccgacggcaa tcgtgtgcgc gatgtcgcgt ttttcggcga
     1321 cggaaatatc gggatgtcga ttttctactt cctgtaaagc gttgacctcc aggcggctta
     1381 tcaaatcgtc agccttgacg cccaagcctt tgcgcccgga catttcgata aaagattttt
     1441 gtttgtcttc ttcggcaagc cggaagccta attcttccgc cgccgagggc gaaagcgcga
     1501 ccatttcata gcttaaatga atgcttgccc gctcgccttt atccggcgaa atcaacgaaa
     1561 cgcctttttt gacgacttcc tgcgggtaag actgccgcga gtcaatcacg ttatagaccg
     1621 tctcgccgct gccgaatttc ggcgcggcgg cgtggtcggc ggcttcggcg gtcgtcaccc
     1681 agacttcttt gccgtcgaaa taagtcgaaa aaagcttgta attaaaatcc aagccgagaa
     1741 cgccgagctt ccagagctga taggcgatgt ctttgcccgt gtaggtgacc gttccgtttg
     1801 agcggacgag aattttgtcg gcttcgtgct cgtcggttcc ggtgtgcgcc tcaaacggca
     1861 tcacccagca gccggcgttt tttccttccg attcgaggcg aataactccg agctgtttca
     1921 tcaaatcgaa ggctttcgcc cagaatttca aatgcagaat ctctgattcg cgcggcagca
     1981 aatcatatcg aatcgagagc cgctccatcg tgttgacgat acactggacg ttgcgcgtgg
     2041 cgacgtaatc ggcaagctcg gcgatttcgt tgccgccttc ttcaatcaaa tgcagggttt
     2101 cggcgcgttt cgctttcagc tcttcgtcgt tttgatattc ctgcccgacg cgcgcgtata
     2161 aatcccagca gtaataatcg aaagtttttc cttcttcgcg caaatcttcg tcgagctgtt
     2221 tgatgtcgtc gagattcatt ttttttatat gaacgaagcc gacgacgaca tcggcgacct
     2281 gcacgcccgt gttgtcgata taatactgcg tctccgtgcg ctcgccgttc gcctgcaaaa
     2341 tccgcagaaa agtatcgcct aaaacggcat tgcgaacgtg accgatatgc gcggctttgt
     2401 tcggattgac cgaagtgtgc tcgaccattc gcttcggcgc gttttcttcg tattttttca
     2461 gtttcgataa aggctcagcc gtcgaagcgt cggctaaaaa tccggcgcgg ttatagaaga
     2521 cgttcaaata tcccgcgccc gccacttcga cgcgcgccac gaaatcttct ttttccagaa
     2581 cggatttcaa agtttcggca atctcgcgcg gatttttctt ttcgcccgtc gcctgcttga
     2641 ttcttttcgc cagttcgaac gcgaccggaa acgccaaatc gccgagctcg gttttcggcg
     2701 gaacttccat cggcggctgc tccagctcga tctgaaaatt ttctccggcg gtttcgacga
     2761 ctttcccgcg caaaatttct tgtaactgta aaaggttcat ctcaaaacaa aatttatcta
     2821 atagaataaa actgaaatta taaaggtttc tatgaagaga tagcaaaagg ctatgccgac
     2881 caaaaatgac cgacacagcc tttttccgca tttaatgcct ctttagcagg cgtttttagg
     2941 gaagcgtttg tttattattt tatttccgcc gacgcaaagc tctttttcaa gaaacccggc
     3001 aaaaatcgga tgtacggagc atatttttgc gtgtatagcc gatattttct ctgtacggaa
     3061 catattttct ctgcacaact catattttct ttgcacaacg agtattttct ttgtacagag
     3121 catactttct ttgcgcagcg ctgtttcccg cgattcctca acgaattgaa accgtcagcg
     3181 aataagtcgt cgcgccgcgc agattgtcca cgcagacgga ttggtcgccc gaatattcga
     3241 tttcggtttg atagctggtg tcgccgcttt caaaaatacg aacctgaccg ctgcgcgagc
     3301 tgacggttgc cgtcagggtt tgtcccgctt tggcattggc gacgtaacag attctgccgc
     3361 ccgtgccgat tctgcccgtc atcgtggcgg aagttctgcc gcgagcgaag cgcacgcgaa
     3421 tatcggcgta gccggtttcg gaa
//
LOCUS       DASWNE010000293         6706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_257342, whole genome shotgun sequence.
ACCESSION   DASWNE010000293 DASWNE010000000
VERSION     DASWNE010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6706
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257342"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..397
                     /gene="xseB"
                     /locus_tag="VGB68_06015"
                     /pseudo
     CDS             203..397
                     /gene="xseB"
                     /locus_tag="VGB68_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125145.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
     gene            complement(566..844)
                     /locus_tag="VGB68_06020"
     CDS             complement(566..844)
                     /locus_tag="VGB68_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048818.1"
                     /translation="MQNQLQNQYAPCPKCSQSVAQKVSFTWWGGVLGPKLLTHVKCGA
                     CGTAYNGKSGKDNTTNIIIYSVAALVIAFFLLLIFFFGLGILTYVLNR"
     gene            complement(889..2226)
                     /locus_tag="VGB68_06025"
     CDS             complement(889..2226)
                     /locus_tag="VGB68_06025"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0048819.1"
                     /translation="MSSNITPADSASRLPAVFRAFKHRNFQLFFSGQIISLTGTWMQS
                     VAQSWLVYRLTGSVALLGLIGFAGQFPVFLLTSFGGAIADKYDRQRILVATQTCSMIV
                     AAILAALALTGTIAVWHLFVLASMAGVINAFDIPTRQSFVVDMVGKEDLLNAIALNSS
                     MFNGARIIGPAIAGILVSAVGEAWCFLGNAVSYIAVITGLLLMRMMQKQAVASEGSAI
                     EKIKEGFGFAAKTAPVRALLLLLGLVSLMGMPYAVLMPIFAGEILHGGVRGLGILMGA
                     SGLGALVAALLLASRKSIRGLGRWVAISSGSFGVFLILFSFSRSFWLSALLLVPVGFS
                     MMLQMASSNTLVQSMVPDELRGRVMAVYSMMFMGMAPLGALMAGSLAGIIGAPYTVVL
                     GGAICIVGSIIFGLRLPKLRVEAREIIVALQMTGGEPASKASFQQPVLVTSKK"
     gene            complement(2474..2977)
                     /locus_tag="VGB68_06030"
     CDS             complement(2474..2977)
                     /locus_tag="VGB68_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048820.1"
                     /translation="MFENDLTRRIAEFLIEIGLQIAPKQLDENTFLFGIFIENGGIFV
                     DESKLAFPGDLLHEAGHLAVMPKYLRAHLSDEIELPGEFDIDVVEVASIAWSYAAALH
                     LDLNPRVVFHEGGYKGNSESLLMNFSLGVYIGVNTLEDTEMTATGENARRLGVSPYPQ
                     MLKWLRD"
     gene            complement(3044..3754)
                     /locus_tag="VGB68_06035"
     CDS             complement(3044..3754)
                     /locus_tag="VGB68_06035"
                     /inference="COORDINATES: protein motif:HMM:NF013214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HEY0048821.1"
                     /translation="MNTYENTFGNNSAADALPEERARFIRKTYLHLAAAILAFVVLEA
                     FLFASGIAGTIMMTMRIGGNFSWLLVLGAFMGVSWLANSWANSQTSKPMQYLGLMLYV
                     IAEAVIFVPMMFIAASFGGGDVIMKAGIVTLGLFLGISSVVFITRSDFSFLGPILAIG
                     GFVALGFIVASILFGFSLGSIFAFIMVAFAGTAILYETSNVMHRYHTSQHVAASLTLF
                     AAIALLFWYILSIFSSRD"
     gene            complement(3855..4268)
                     /locus_tag="VGB68_06040"
     CDS             complement(3855..4268)
                     /locus_tag="VGB68_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY0048822.1"
                     /translation="MERFLGRYEPHFYALLRIVSGFLFLLHGTQKFFSFPPMPPQMPS
                     APLNGLMIAAAAIEVAAGLLILIGLFTSLAAFIASGMMAVAYFMAHQPQGALPASNGG
                     DAAVLFCFIFLYMSARGSGILSVDSARRGGTGERR"
     gene            4443..4745
                     /locus_tag="VGB68_06045"
     CDS             4443..4745
                     /locus_tag="VGB68_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048823.1"
                     /translation="MNRRKNSGSSDVARAKPPFTALHKNKGNREKFRWLIVNSQLLIL
                     NCLTKDTEHQFRIYNLEFTIFPFFIFVQSRRSRQGAGGIKINAANFSASFFPLIAG"
     gene            5044..5499
                     /locus_tag="VGB68_06050"
     CDS             5044..5499
                     /locus_tag="VGB68_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048824.1"
                     /translation="MRRMTRTLIGLALLMALVAAGLTTGSQAQGTSGQQNTSQQNQNN
                     SNKKNKKNKNKNGNSNRNNNGNMNGNTNSNMNTNSNMNMNSNMNSNTNANTNSDMNMN
                     NANGNQNANSRNRNTNGNTNGNTNSNTNSNANTNMNTNTNANTSPTPRP"
     gene            complement(5569..6090)
                     /locus_tag="VGB68_06055"
     CDS             complement(5569..6090)
                     /locus_tag="VGB68_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048825.1"
                     /translation="MKYLIVAFVLFLSVDLAFAQKHEYTIRARSERLIVKHKGKAHTL
                     RLGSDKIDAAKITSAEILFANRKGGFTYLLVDVAGQSKEKSDDRQCGAGTESNLIWIK
                     LDAAWKIIDARSQRYESCWSGITSDDGYRIKGNLLTIEFDNFRENVTVKLSYDADAPE
                     KGFEKKSSAINNQ"
     gene            complement(6101..6526)
                     /locus_tag="VGB68_06060"
     CDS             complement(6101..6526)
                     /locus_tag="VGB68_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048826.1"
                     /translation="MQQNIINSQPRFCYKCHYPAGNFDKKCQRCGGVVRTKATIRILG
                     GLLVFLGGFISAMMAFVGLFVFGSLAQTPASRFNGSETELLFVVGIISLTFIVGVSFA
                     FAGLWQIIFGRRNTVIVWVSIGLVIVLFVIGRIFVMMTR"
BASE COUNT         1947 a         1727 c         1577 g         1455 t
ORIGIN      
        1 caaaacttgc aggagatgct gaaaaaggtt gaagatattt ttaaataagt taacaggaag
       61 tttgcagata gtttgcaggt agttgcagga agcttaagac aactgccgga gattgaaact
      121 ccgaaactgt ttttaagtat ctgcaactac ctgcaaactt cctgcaaact atctgcaagc
      181 ttcccgttaa ctccaaattt ttatgagtca aaaaaatcag acgtttgaag cgtctttatc
      241 gaatctggaa aaaatcgtgc gccgtctgga agaaggcgat ttgccgctcg aagaaagcct
      301 caagcttttt gaagacggcg tccgcctgtc gcgcgaatgt caggaaagac tgaatcaggc
      361 ggaaagaaga atcgaagttt tgctcaagga cgaggacggc aatccggctc tgaacgcaat
      421 cggacgcgaa gacgccgaag aagcacagcc gaaaatcaaa cgccgcattc gcttcgatga
      481 cgaaagcgac aacgacgagg acgagacgcc cttttaagaa tttcagcgaa tcgcgcaatc
      541 gatttttttc gtttcgtttg aaaaattacc tgtttaatac atacgtcaaa atgccgagtc
      601 cgaagaaaaa aatcagcagc aggaaaaacg cgataaccag ggcggcaacc gagtaaataa
      661 taatattcgt cgtgttgtcc ttgccggatt tgccgttata ggctgtgccg caagcgccgc
      721 atttgacgtg cgttaaaagc ttcggaccta aaactccgcc ccaccacgtg aaagacactt
      781 tctgggcgac tgactggctg catttcggac acggggcata ttgattctgc aattgattct
      841 gcataagtag cgcctattct aaaataaatc gccgccggct ttcaatattt attttttaga
      901 tgtgaccaga accggctgtt gaaacgacgc tttcgatgcc ggctcgccgc ccgtcatttg
      961 cagcgcgacg atgatttcgc gcgcttcgac gcgcagcttc ggcaggcgca agccgaatat
     1021 aatcgagccg acaatgcaaa ttgcgccgcc cagcacgacc gtgtaaggcg cgccgataat
     1081 tccggcgagc gagccagcca tcagcgcccc gagcggcgcc atgcccataa acatcatcga
     1141 ataaaccgcc atcacgcgac cgcgcagctc gtccggaacc atcgattgca ccagcgtgtt
     1201 tgacgaagcc atttgcagca tcatcgaaaa accgaccggc accagcagca gcgccgacag
     1261 ccagaacgag cgcgaaaacg aaaacaaaat cagaaacacg ccgaagctgc ccgacgaaat
     1321 cgcgacccat cgacccaagc ccctgatgct tttccgcgag gctaaaagaa gcgcggcaac
     1381 gagcgcgccc aaaccgctcg cgcccattaa aattccaagc ccgcgcacgc cgccgtgcaa
     1441 aatctccccg gcgaaaatcg gcatcagaac ggcatacggc attcccatta agctgaccag
     1501 cccgagcagc agaagcagcg cgcgaaccgg cgcggttttc gccgcaaagc cgaagccttc
     1561 ctttattttc tcgattgccg agccttcgct ggcgaccgcc tgtttttgca tcattcgcat
     1621 caataaaagt ccggtaatga cggcgatata gctgacggca ttgcccagaa agcaccacgc
     1681 ctcgccgacc gccgaaacca gaattccggc aatcgccgga ccgataatcc gcgcgccgtt
     1741 aaacatcgaa gaattcagcg caatcgcgtt aagcaaatct tctttgccga ccatatcgac
     1801 gacgaaagac tggcgcgtcg gaatatcgaa agcgttgatg acgcccgcca tcgatgccag
     1861 cacgaacaga tgccagacgg cgattgtgcc ggtcagcgcc agcgctgcca gaatcgccgc
     1921 gacaatcatc gaacaggttt gcgtcgcgac gagaattcgc tgccggtcgt atttatccgc
     1981 aatcgcgccg ccgaaagacg tgagcagaaa aaccggaaat tgtccggcga aaccgattaa
     2041 tccgagcagc gcgaccgagc ccgtgaggcg atatacaagc cagctttgcg cgaccgactg
     2101 catccacgtg ccggttaagg aaatgatttg accgctgaaa aacagttgaa aattgcgatg
     2161 cttaaaggcg cgaaaaacgg caggcagcct ggaagccgaa tcggctggcg tgatgtttga
     2221 agacattaaa attcccttgc tcgtaatatt ttatgcgaat cgaaaataga aataaaagaa
     2281 aaaaaacgat tttctttgcc ttcttcgctt ctcaagcgaa acatttttcg caaaattgaa
     2341 acgttatcag gaaaaacaat ttcccgcttc ggaggcaaag agcgcaaagt ctgacgcgca
     2401 gtttttcaac tatcctttta ttcacatatt ttatagttta acgacgagcg gcggataatt
     2461 tcaacgacgg gatttaatcg cgaagccatt tcaacatttg cgggtaaggc gaaacgccga
     2521 gccggcgcgc gttttcgccc gtcgccgtca tttccgtatc ttccagagta ttgacgccga
     2581 tgtaaacgcc gagactaaaa ttcatcaaca aagattcgga gtttcccttg taaccgcctt
     2641 cgtggaaaac gacgcgcggg tttaaatcca gatgcaaagc cgccgcgtaa gaccaggcga
     2701 ttgaagcgac ctcgacgaca tcgatatcga attcgccggg cagctcgatt tcatcgctta
     2761 aatgagcgcg caagtatttc ggcattaccg ccagatgacc ggcttcgtgc agcaaatcgc
     2821 cgggaaacgc gagcttcgat tcatcgacaa aaatcccgcc gttttcgata aaaatgccga
     2881 agagaaacgt attttcgtcc agttgtttcg gagcgatttg cagcccgatt tcgattaaaa
     2941 attccgcgat tcgccgcgtc aggtcatttt cgaacatctt ttcgattata aacgaaaaag
     3001 gcggattcag tgtccgcctt tgcccgtaaa attttccttt cgattaatcc ctggaactga
     3061 aaatgctcag gatataccag aacagcaggg cgatagccgc aaacagcgtc aacgaagccg
     3121 cgacgtgctg cgacgtgtga taacggtgca tcacgtttga ggtttcatac aaaatcgccg
     3181 ttcccgcaaa ggcaaccatt atgaaggcga aaatgctgcc caggctgaag ccgaacaaaa
     3241 tgctcgccac gataaatccg agcgcgacga agccgccgat tgccaggatc ggacctaaaa
     3301 acgagaaatc ggaacgagtg atgaaaacca ccgacgagat gcccagaaat aaaccgagcg
     3361 tgacgatgcc ggctttcata atcacgtcgc cgccgccgaa actcgcggcg atgaacatca
     3421 tcgggacgaa aatcaccgct tcggctatga cgtaaagcat cagtccgaga tactgcatcg
     3481 gtttcgacgt ttgcgaattc gcccagctgt ttgccagcca cgaaacgccc ataaacgctc
     3541 cgagcacgag cagccacgaa aagttgccgc cgatgcgcat cgtcatcata atcgtgccgg
     3601 cgatgccgga ggcaaataaa aacgcttcca gaacgacgaa cgccagaatc gccgccgcca
     3661 gatgaagata agttttgcgg atgaaccttg cgcgttcttc cggcaaggcg tctgccgccg
     3721 agttgtttcc gaatgtattt tcgtatgtgt tcataatttt cctctgtcac tacaggacat
     3781 ttgttattta gacgataaat cgaaaaattt gtttgcccgc ccgctgttaa ttttacgtta
     3841 aaagcgaatt tatatcacct gcgctcgccg gttccgccgc gccgcgccga atcgacgctc
     3901 agaatgccgg agccgcgcgc cgacatatag agaaaaataa agcaaaacag caccgccgcg
     3961 tcgccgccgt tagaagccgg cagcgcgccc tgcggctggt gcgccataaa ataagcgacc
     4021 gccatcatac cgctggcgat aaatgccgcg aggctcgtaa acaacccgat aagaatcaac
     4081 agcccggcgg cgacttcaat cgcggcggcg gcaatcatca gcccgttcag cggcgcggac
     4141 ggcatttgcg gcggcatcgg cggaaagctg aaaaatttct gcgtgccgtg cagcaaaaac
     4201 aaaaatcccg atacaatgcg caaaagcgcg taaaaatgcg gttcatatct tcccagaaat
     4261 ctttccatct gtaatctcct tatttgaaac cggacaaccg cttactatca taactcaggt
     4321 tatcaagctt attaaataaa aaagggcgaa gaaactttct tcgccgccaa tattcactga
     4381 aagagaaatt ttatctgcat cgacaatgtt aatctgcccg gtttcggatt tgccgaaggc
     4441 tggtgaatcg tcgcaaaaat tcaggctcga gcgatgtcgc ccgcgcaaag ccgccgttca
     4501 ctgctttgca caaaaataaa ggaaatcgag aaaaattccg atggctaatt gtaaattctc
     4561 aattattaat tctaaattgc ttaacaaaag ataccgaaca tcaatttaga atttacaatt
     4621 tagaatttac aattttccct ttctttattt ttgtgcaaag ccgccgttca cggcaaggcg
     4681 cgggcggcat aaaaataaac gctgcaaact tttccgcttc gttctttcct ttgattgccg
     4741 gatagggttt ttcgcggact aaactcgcgg agcttcagct tgccttagac aaacagtgcc
     4801 gggcgagcgc gaaaaaagac ggtttcggaa aaatatttct ccgggcggcg tttttccttt
     4861 gtattttgtc cgtttgaaga cataaaagcg cttttcgttc tgctaggatg aaggtaaaca
     4921 gcgcgtcgga aaacaaagcc tcaaacgcgg gttgaaaccg gcgcagaaaa gcggattttg
     4981 acagggaacg ggcgctttga aaaaagctca tcgtcttaat ttggcaaatg gagggaaaat
     5041 aaaatgcgga gaatgacacg aaccttaatc ggactggctt tactgatggc tttggtcgcg
     5101 gcgggtttga ccacgggcag ccaggctcag ggcacgagcg gtcaacaaaa caccagccag
     5161 caaaatcaaa acaacagcaa caaaaaaaat aagaaaaaca aaaacaaaaa cggcaattca
     5221 aaccgcaata acaacggaaa catgaacggc aatacgaatt caaatatgaa tacgaattca
     5281 aatatgaata tgaattcaaa tatgaattcg aacacgaacg cgaatacgaa ttccgacatg
     5341 aatatgaata acgccaacgg taatcagaat gccaacagcc ggaatagaaa cacgaacgga
     5401 aacacaaacg gaaacacaaa ctcgaacaca aactcgaatg cgaatacgaa tatgaatacg
     5461 aacacgaacg ccaacacgag tccgacgccg cgcccgtagt ttgagaaaac gagtttgctg
     5521 ataagcagtc aagcgggaat ctttacggaa agattcccgc ttttccattt attgattatt
     5581 tattgccgac gattttttct cgaatccttt ttccggcgcg tccgcgtcgt aagacaattt
     5641 taccgttaca ttttcacgaa aattatcaaa ttcgatggtt aaaagattgc cttttatccg
     5701 gtagccgtca tcagatgtaa ttccggacca gcaggattcg tagcgctgcg agcgggcatc
     5761 gattattttc cacgccgcgt cgagcttaat ccaaatcaga ttcgattcgg ttcccgcgcc
     5821 gcattgccgg tcgtccgatt tttccttcga ttgaccggcg acgtcaacca gtagatatgt
     5881 aaacccgcct ttgcgattag cgaataaaat ttccgcgctc gtgattttag cggcgtcaat
     5941 cttatcgctg ccgagccgga gcgtgtgcgc tttgccttta tgtttgacga ttaatcgctc
     6001 gcttcgggcg cgaatcgtgt attcgtgctt ttgcgcgaaa gccaaatcga cgcttaaaaa
     6061 taaaacgaaa gcgacaatca aatatttcat aagctccggg ctatctcgtc atcataacga
     6121 aaattctgcc gatgacaaac aaaacaatca ccaagccgat tgaaacccag acgataaccg
     6181 tattgcgcct gccgaaaatg atttgccaca atccggcgaa cgcaaacgaa acgccgacga
     6241 taaaagtcag gctgatgatg ccgacgacga atagcagttc ggtttccgaa ccgttaaagc
     6301 ggctcgccgg agtttgcgcc agcgagccga aaacaaacag cccgacaaac gccatcatcg
     6361 ccgagataaa gccgccgaga aagacaagca agccgcccag aattctgatg gtcgccttcg
     6421 tccgcaccac gccgccgcag cgctggcatt ttttatcgaa attgcccgcc ggataatgac
     6481 atttgtaaca aaaacgcggt tgtgaattaa ttatattttg ctgcatattg aataaataaa
     6541 cgtaaaaata tttactcaat cacagacggc aatcgataga atttttgctt tgcgatttaa
     6601 tggcggtttc tgctcgcccg cattcgtttc ggtccgaacc agagccagaa gccggttatc
     6661 gtgaagctaa gcagggcaaa tccggtgatg gaggtataga cgactt
//
LOCUS       DASWNE010000294         8537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_644897, whole genome shotgun sequence.
ACCESSION   DASWNE010000294 DASWNE010000000
VERSION     DASWNE010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8537
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644897"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="VGB68_06065"
     CDS             <1..503
                     /locus_tag="VGB68_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048827.1"
                     /translation="TNYNFGSIRYESRKVFVTAFYFPSRYECDVSFGLFDDKSQSYNI
                     GDLTGSADDFDFSPNYTFEKLSQAEILERNISWFASLLQRYGSDYLKGDVRAYKKLEE
                     RSNRRSKEYTQDLTNKQLRNKAASAWEQKDFAVVVESYKSMKKFLSAAEKKKLEYSEK
                     MLNKND"
     gene            496..1704
                     /gene="trpB"
                     /locus_tag="VGB68_06070"
     CDS             496..1704
                     /gene="trpB"
                     /locus_tag="VGB68_06070"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEY0048828.1"
                     /translation="MTELKNFQPNEHGYWGEFGGRFVPETLMSPLEELLGAYFAARDD
                     AGFQTEFLRLLKDFSGRPTPLFHARRLSETLGGARIYLKREDLGHTGSHKINNAIGQV
                     LLARKMGKKRIIAETGAGQHGVATATVCALFGLECVVYMGTEDMRRQSLNVFRMQLLG
                     AEVRGVDSGSRTLKDAINEALRDWVTNIDSTYYLLGSALGPHPYPLMVRDFQSVIGRE
                     AREQILEKTGRLPDLLVACIGGGSNAIGLFHPFLQDESVKMIGVEAGGKGNALGEHAA
                     RFNSSGGGKIGVLQGTKSYILQDERGQIALTHSISAGLDYASIGPEHAFLHDIGRVEY
                     VSASDAEAVGAFQTLSQTEGIIPALETSHGIAHALKIAPEMSPQQVMIVNLSGRGDKD
                     VNQVQEMLEK"
     gene            1701..2048
                     /locus_tag="VGB68_06075"
     CDS             1701..2048
                     /locus_tag="VGB68_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048829.1"
                     /translation="MNRPLTEQEKEWMIRGLKTLETGEYFGGGNWIDTKANEIKPLDE
                     PVNSEYFLNQIDNLRVVGKCNCGAPDCHTIQFQHFDKGKSASLVCYHTEDNRMLIILI
                     NEDTGKLAELEII"
     gene            2048..3265
                     /locus_tag="VGB68_06080"
     CDS             2048..3265
                     /locus_tag="VGB68_06080"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0048830.1"
                     /translation="MIFIFYFFAAVLVFLSYKSLRGGIAYLNFFKRELAKPKSDFEPF
                     VSVIVPCRGLDKDLEKNLNALFEQRFPAYEVIFVTGDEKDESVKIIKEISRRGAKSAD
                     SKLVISGKTSDESQKVHNLREAVLQVSGDSEVFVFVDSDARPGKDWLRNLIAPLADEK
                     IGATTGYRWFISKKFGFASEMRSVWNASIASALGANPKSNFCWGGSMAMRRATFEQLR
                     MREKWRGVLSDDFTVTRALKEANLPIYFVPQALTVSVEDCSLGELLEFTTRQMKITRV
                     YAAHLWKQSFAGAFLFNLVFVWGILIIAFNPVNTVSFWFALISLLLISAFSTGKAWLR
                     LKAAKLILRDYEKDLKRQFRTQNTLWIFSPALFLYNSTCALFSRKILWRGIAYELKSP
                     DKTIIIQENTTSF"
     gene            3336..4706
                     /locus_tag="VGB68_06085"
     CDS             3336..4706
                     /locus_tag="VGB68_06085"
                     /inference="COORDINATES: protein motif:HMM:NF014503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048831.1"
                     /translation="MLNFLILRRARFFILLSIVFIFAQLAPAQNTETKATPAKTGKMP
                     VIIIPGLIGSELVNRETGESVWFHLQRAKKDDLRLPISANIARNRDNLVTGDILRNVK
                     LVKFLPETQIYERLIEALKADGYAEAKWDAPAATDVFYVFPYDWRRDNVESARILIQK
                     IQSAKTKLRNPNLKFNIIAHSMGGLISRYAVMYGNTDLPAGKRRIVPNWAGAKHFNKV
                     FLVGTPNEGSLPSLNSLINGFSFLGGGLNLPFVQNLTRFDIFTIPAIYQLLPQAGTLR
                     AFDENLKPLEIDIYNVETWEKYEWAAYQDEDFPKKFSAAEQRNARAYLRAVLERARRF
                     HEALRSTSLTKSPVPVYFLGAECKPTLDGMVIYRDAKKNVWRTLFKPDSFERADGTKV
                     TDKELEPLLYSPGDGTVTKRSFLTAAIRNISNNEQTSFPVCEEHQNLTGNAEIQKTMF
                     AVLDEK"
     gene            4730..5851
                     /locus_tag="VGB68_06090"
     CDS             4730..5851
                     /locus_tag="VGB68_06090"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0048832.1"
                     /translation="MSKIGQFAQLIRDTGRAKKLDTVIFFVTSRCNAACETCFYHEKL
                     NQKGDLTLAEIERVSLSMPEITDLWLSGGEPTLRRDLNRIIDLFVANNRINRLIIPTN
                     ALVKSKVFETVEQALSHEQITDLYLNIALDGYGETHDKIRGVPRNWEKALDCIEHLYP
                     LKEKFGSRFRLNVNTVICAENYTEIEKLSEFLWRNFKLDGQYFNVVRGDAKSGDSIKA
                     VPNEALPKIYKHAANLTKRYGERMFAGDDFSERFVKSVAYVGTIMTHYRHQHANFDKP
                     TAWAFPCTAGATTAVIDYDGDVRACELREKFASLRDFDFDFKRLWADISRTDEIDAID
                     DGRACWCTHVCFIHDSLRHSRKALLVEVPKNYLTRENWV"
     gene            5848..6486
                     /locus_tag="VGB68_06095"
     CDS             5848..6486
                     /locus_tag="VGB68_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEY0048833.1"
                     /translation="MIKSLFEHDDEQPQPKENKPETETEESIRQQLDELNLLAETPGA
                     DDEVETVILPRIEQVYANANEPPGNVFPENRFYEADTETPALETAPMPPEAAPTPQIP
                     TATQYRNVLQDELAPRNESVSQAENVYQSENAAQTEARSLTTAETIRQSGMAWSAAIG
                     LFGSVLFMMILGWFFDLLTGASPLGLVGGIIVGSAIGFYQFFRTTSQIFKKD"
     gene            6767..7204
                     /locus_tag="VGB68_06100"
     CDS             6767..7204
                     /locus_tag="VGB68_06100"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEY0048834.1"
                     /translation="MSEISEPVTTEENPRTLQLSHRRILWIMAIVLIVGVALSLIYSR
                     RSFTLGLIIGGGLACLNYYWLRLSLKNIFDTMAAAGEKPRFLAANYFLRYATLGILVA
                     IVFITETASITGVLLGLSSIAPAVVVEGLIRIFTSSSSKREEL"
     gene            7206..8126
                     /gene="atpB"
                     /locus_tag="VGB68_06105"
     CDS             7206..8126
                     /gene="atpB"
                     /locus_tag="VGB68_06105"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEY0048835.1"
                     /translation="MLLFAEGGHHTPAIVEFINHYVGEPVHHFQMAYTKPFWDNYVFG
                     YFGTTADKVFGAYTPENAVPWYTIMFIIACILTVAVIWIFKGKLSEDDPSNGQLTLEA
                     GFLALKDMVVSVIGEHGFKYFPVVASFAVLVLISNLMGLFPLFMSPTAHVSVTFALGI
                     ASFIYYNYIGIRENGLISYLAHFAGPKLPLIMMLLITPLIFAIELFSNMIRPMTLGIR
                     LFANMYADEQVASIISGLFPPFTQFLVPIILLPLAVFIALVQTFVFTLLSMIYISGVS
                     HAPHDHHEHAGNEHAHTGAHGEVPAATASA"
     gene            8145..8324
                     /locus_tag="VGB68_06110"
     CDS             8145..8324
                     /locus_tag="VGB68_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048836.1"
                     /translation="MNDKSNRVYHSPVHDSLVHDSQFGFDKPDGNKAFYSSFVSTLTG
                     AARLPRQCAAYRKLT"
BASE COUNT         2140 a         2096 c         2194 g         2107 t
ORIGIN      
        1 aaacaaatta caacttcggc tcgatccgct atgaatcaag gaaagttttt gttacagcgt
       61 tttacttccc gtcgcgatat gaatgtgacg tatcttttgg gctttttgac gataaatcac
      121 aatcctataa tataggtgat ttgactggct cggcagatga ttttgatttt tcgccgaatt
      181 atacttttga aaagttaagt caagccgaaa tccttgagcg aaatatttcg tggtttgcct
      241 cacttctaca aagatacggc tctgattatc tgaaaggcga tgtcagggct tataagaaat
      301 tagaagaaag aagcaataga agaagtaaag aatatactca ggatttaact aataagcaat
      361 tgcgaaacaa agccgcatcc gcttgggaac aaaaggattt tgccgttgtc gtcgaaagct
      421 acaaatcaat gaaaaaattt ctctcggcgg cggaaaagaa aaaattagaa tactcggaaa
      481 agatgcttaa caagaatgac tgaattaaaa aatttccaac cgaacgaaca cggttactgg
      541 ggcgagttcg gcgggcgttt tgttcccgaa acgctgatgt cgccgctcga agagcttttg
      601 ggcgcgtatt ttgccgcccg cgacgacgca ggctttcaaa ccgaattcct gcgtttatta
      661 aaggattttt ccggtcgccc gacgccgctt tttcacgctc gccgactttc ggaaacgctg
      721 ggcggcgcgc ggatttatct caaacgcgag gatttgggac acaccggctc gcacaaaatc
      781 aacaacgcca tcgggcaggt tttgctggcg cgaaaaatgg gcaaaaagcg cattatcgcc
      841 gaaaccggag ccggtcagca cggcgtggcg acggcgaccg tctgcgcttt gttcgggctg
      901 gaatgcgtcg tttatatggg cacggaagat atgcgccggc agtcgctgaa cgtttttcgg
      961 atgcagcttc tcggcgccga ggtgcgcggc gtcgattccg gctcgcggac gctgaaagac
     1021 gcgataaacg aagctttgcg cgattgggtg acgaacatcg attcgactta ttacctgctc
     1081 ggctcggcgc tcggtccgca cccgtatccg ctgatggtca gagattttca aagcgtcatc
     1141 ggacgcgaag cgcgcgagca gattctggaa aaaaccggaa ggctgcccga tttactcgtc
     1201 gcctgcatcg gcggcggctc aaatgccatc gggctttttc acccgttttt gcaggacgaa
     1261 agcgtgaaaa tgattggcgt cgaagccggc ggaaagggca atgctttagg cgagcacgcg
     1321 gcgcgtttca attcatcggg cggcggaaaa atcggcgtgc tgcaaggcac gaaaagctat
     1381 attctgcagg acgagcgcgg gcaaatcgct ctcacgcatt cgatttcggc gggcttggat
     1441 tacgcttcga ttggacctga gcacgccttt ctgcacgaca tcgggcgcgt cgaatacgtc
     1501 tcggcttcgg acgcggaagc agtaggagcg tttcagactt tatcgcagac cgaaggcata
     1561 atccctgcgc tggaaacctc gcacggcatc gcgcacgcgc tgaaaatcgc gccggaaatg
     1621 tcgccgcagc aagtgatgat cgtgaatctt tcggggcgcg gcgacaagga cgtcaatcag
     1681 gttcaggaaa tgctggaaaa atgaatcgcc cgctgacgga acaggaaaaa gaatggatga
     1741 ttcgaggact gaaaacgctc gaaacgggcg aatacttcgg cggcggaaat tggattgaca
     1801 caaaagcaaa tgaaataaag cctttggacg aacccgtaaa ttccgaatat tttctaaatc
     1861 aaatcgataa tcttcgcgtc gtcggcaaat gcaattgcgg cgcgccagat tgtcatacaa
     1921 tccaatttca acattttgat aaaggaaaat cagcctcgct cgtctgttat cacacagaag
     1981 ataatagaat gttaattatt cttataaatg aagatacggg aaagctggcg gagcttgaaa
     2041 ttatctgatg atatttattt tctatttctt cgctgccgtt ctggttttcc tgagctacaa
     2101 atccctgcgc ggcggaattg cttatctaaa ttttttcaag cgcgagcttg ccaagccgaa
     2161 atccgatttc gagccttttg tttccgtaat cgttccgtgt cgcgggctcg ataaagattt
     2221 ggagaaaaat ctgaacgcgc ttttcgagca gcgttttccg gcttatgaag taattttcgt
     2281 aactggcgac gaaaaggacg aatcggtaaa aattatcaaa gaaatctccc gccgaggcgc
     2341 aaagagcgcc gactcaaaac ttgtaatttc cggaaaaacc agcgacgaaa gccagaaggt
     2401 tcacaatctg cgtgaagccg tcctgcaggt ttccggcgat tcggaagttt ttgtctttgt
     2461 cgattccgac gcgcgtcccg gcaaagactg gctgagaaat ttaatcgcgc ctttagcgga
     2521 cgaaaaaatc ggcgcgacga ccggctatcg ctggttcatc tcgaaaaaat tcggctttgc
     2581 ttccgagatg cgttcggtct ggaacgcttc gattgcttcg gcgctgggcg caaatccgaa
     2641 atcgaatttc tgctggggcg gctcgatggc gatgcgccgg gcgacatttg aacaattgcg
     2701 gatgcgcgaa aaatggcgcg gcgttttgtc cgacgatttt accgttacgc gcgcgttgaa
     2761 ggaagcgaat ttgccgattt attttgtgcc gcaggcgctt accgtttcgg tcgaagattg
     2821 ttcgctcgga gagcttctgg aatttacgac ccggcagatg aaaatcacgc gcgtttacgc
     2881 cgcgcatctc tggaaacaat cgtttgcggg agcgtttctt ttcaatctgg ttttcgtctg
     2941 gggaattttg attattgctt ttaatccggt aaacacggtt tcattctggt ttgcgctgat
     3001 cagcttgctt ttgatttccg cgttcagcac cggtaaggcg tggcttcgtc tgaaagccgc
     3061 gaaattgatt ttgagagatt atgaaaagga tttgaagcgg caattccgga cgcagaatac
     3121 gctctggatt ttttcgcccg ctttgtttct ctacaactcg acctgcgctc ttttctcgcg
     3181 caaaatcctc tggcgcggca tcgcttacga attaaaatcg cccgataaaa ccattattat
     3241 tcaggaaaat acaacttcct tttgattgct ttcatcagag ggctaaggta aatttttaat
     3301 agtttaaatt tattaccgaa caaggaaaag ggtttatgct taatttttta atactgcgcc
     3361 gggcgcgttt ttttatatta ctgtcgattg tttttatttt cgcgcagctt gcgcccgcgc
     3421 aaaataccga aacgaaagcg acgcccgcga agacaggcaa aatgccggtc atcatcattc
     3481 ccggcttaat cggctcggaa ctggtcaata gagaaacggg cgagagcgtc tggtttcacc
     3541 tgcaaagggc gaaaaaagac gatttgcgcc tgccaatctc ggcgaatatc gcgcgcaacc
     3601 gcgacaacct cgtgacgggc gacattttgc gcaacgtcaa gctcgtcaaa tttttaccgg
     3661 aaacccagat ttacgagaga ttaatcgagg cgctgaaagc cgacggctac gccgaagcca
     3721 aatgggatgc gcccgccgcg accgacgttt tttatgtttt cccttatgac tggcggcgtg
     3781 acaacgtcga atccgcgcgg attttaattc agaaaataca gtcggcgaaa accaaattaa
     3841 gaaacccgaa cctgaaattt aacattatcg ctcattcgat gggcggcttg atttcgcgct
     3901 acgcggtgat gtacggcaac acggatttgc ctgccggaaa gcgtcgcatc gtgccgaact
     3961 gggcgggcgc gaaacatttc aacaaggttt ttctcgtcgg cacgccgaac gaaggctcgc
     4021 tgccgtcgct caactcgctt atcaacggtt tttcgtttct cggcggcggt ctgaatctgc
     4081 cttttgttca gaatttgacg cggtttgata ttttcacgat tccggcgatt taccagcttt
     4141 tgccgcaggc gggaacgctt cgcgcttttg acgaaaatct aaaaccgctg gaaattgaca
     4201 tttataatgt agagacttgg gaaaaatacg agtgggcggc ataccaggac gaggattttc
     4261 caaaaaaatt cagcgccgcc gaacagcgca acgccagggc ttatctgcgc gccgtgctgg
     4321 aacgcgccag acgctttcac gaagcgctcc gttcgacgtc tttaaccaaa agtccggttc
     4381 cggtctactt tttaggcgcg gaatgtaaac cgacgctgga cgggatggtg atttatcgcg
     4441 acgcgaaaaa gaacgtctgg cgcacgcttt tcaagcccga ttcgtttgaa cgcgccgacg
     4501 gcacgaaggt caccgacaag gaattggagc cgctgcttta ttcgccgggc gacggaacgg
     4561 tgacgaaacg ctcgtttctg acggcggcaa tccgaaatat cagcaataac gaacagactt
     4621 cttttccagt ctgcgaagaa caccaaaatc tgaccggcaa cgcggaaatt caaaaaacta
     4681 tgtttgccgt tctggacgaa aaataaaatt tggaagaaaa tcatctgaca tgagtaaaat
     4741 cggtcagttt gcgcaattga ttcgcgacac cgggcgcgcg aaaaagctcg atacggtgat
     4801 tttcttcgtt acctcgcgct gcaacgccgc ctgcgaaacc tgtttttatc acgaaaaact
     4861 caaccaaaag ggcgatttga cgttggcgga aatcgagcga gtctcgcttt cgatgccgga
     4921 aattaccgat ttatggcttt ccggcggcga gccgacgctg cgccgggatt taaacaggat
     4981 tatcgatttg tttgtcgcca acaaccggat taatcgcctt attattccga ccaacgccct
     5041 tgttaaatcg aaagttttcg aaacggtcga gcaggcttta tcacacgaac aaatcaccga
     5101 tttatattta aacatcgcgc tcgacggcta cggcgagacg cacgataaaa ttcgcggcgt
     5161 gccgcgcaac tgggagaaag cgctcgactg catcgagcat ctttatccgc tgaaagaaaa
     5221 attcggctcg cggtttcgcc tgaacgtcaa tacggttatc tgcgccgaaa attacacgga
     5281 aatcgaaaag ctttccgagt ttctgtggcg aaactttaag ctcgacggtc agtactttaa
     5341 tgtcgtccgc ggcgacgcga aatccggcga ttcgataaaa gccgttccga atgaggcgct
     5401 gccgaaaatc tacaaacacg ctgccaatct gaccaaacga tacggcgaac ggatgtttgc
     5461 cggcgatgat ttctccgaac gctttgtcaa atcggtcgct tacgtcggca cgattatgac
     5521 gcattatcgt catcagcacg cgaatttcga taagccgacc gcgtgggctt ttccctgtac
     5581 ggcgggcgcg acgaccgccg tcatcgacta cgacggcgac gtgcgcgcct gcgagctgcg
     5641 cgaaaaattt gcgtcgctca gggatttcga ttttgatttc aaaaggcttt gggcggatat
     5701 ttcaagaacc gacgagattg acgccatcga tgacggtcgc gcctgctggt gcacgcacgt
     5761 ctgctttatt cacgattcgc tccggcattc gcgcaaggcg ctgctcgtgg aagttccgaa
     5821 aaattatctg acgcgggaga attgggtatg ataaaaagtc tgtttgagca cgacgacgaa
     5881 caacctcagc cgaaagaaaa caaaccggaa accgaaaccg aagaatcaat caggcagcag
     5941 ctcgacgaac tgaatctttt ggcggaaaca cccggcgcgg acgacgaggt agaaaccgta
     6001 atcctgccga gaatcgagca ggtctatgca aacgcaaatg agccgcccgg taacgtcttt
     6061 cccgaaaacc gtttctacga agccgatacg gaaacgcccg cgctggaaac cgcgccgatg
     6121 ccgcccgaag ccgcgccgac gcctcagatt ccgacggcga cgcaataccg gaacgttctc
     6181 caggatgaac ttgcgccaag aaatgaaagt gtttctcaag ctgaaaatgt ttaccagagt
     6241 gaaaacgccg ctcaaaccga agccaggtct ttaacgaccg ccgaaacgat tcggcagagc
     6301 ggaatggctt ggagcgcggc aatcggtctg ttcggctcgg ttctgtttat gatgattctc
     6361 ggatggtttt tcgatttgct gaccggcgct tcgcctctcg gactggtcgg cggaatcatc
     6421 gtcggctcag ccatcggctt ttatcaattt ttccgcacca catcgcaaat cttcaaaaag
     6481 gactaggcat aaagcccgtc ctatcaggca atggcgctgc aaagcgtaag aggaattcgc
     6541 gacgctgtat ttaatcaggt gaattaggat tggtcaggat aaattcaaat aaaatcctga
     6601 ccggtcttat tcaatactaa aaaccggcgt cccgatttcg ttaaacaatt ccctgctcgc
     6661 cgcgcgctta agcttcagcc tgatacggta atttgagaaa aattgcacaa atgcccgttc
     6721 gggctttgaa attttcaccg aagccttata aaatagtggt ttcgcaatga gcgaaatttc
     6781 cgaacctgta acaactgaag aaaatccgcg cacgttgcag ctttcgcacc ggcggatttt
     6841 atggattatg gcgattgttt taatcgtcgg cgtcgcgctc agtttgattt actcgcggag
     6901 aagtttcacg ctcggcttga taatcggcgg cggcttggct tgtctgaatt attactggct
     6961 caggctttcg ctcaaaaata tatttgacac aatggctgcg gcaggcgaaa aaccgcgctt
     7021 tttagccgca aattattttt tgcgctacgc gacgctcggc atactcgtcg ccatagtttt
     7081 tattacggaa accgcttcga ttaccggtgt tttgctgggg ctttcaagca tcgcgccggc
     7141 agtcgtcgtc gaaggtttaa tacgaatttt tacatccagc tcttctaaac gagaggaact
     7201 ttaaaatgct tttatttgca gaaggcggac atcatacgcc cgctatcgtc gagtttataa
     7261 atcattacgt gggcgagccg gttcaccatt ttcagatggc ttacaccaag ccgttctggg
     7321 acaattacgt tttcggctat ttcggcacga ccgccgataa agtcttcgga gcttatacgc
     7381 cggaaaacgc ggttccctgg tacacgatta tgtttatcat cgcgtgtatc ctgacggtgg
     7441 cggtcatctg gattttcaaa ggaaagcttt ccgaagacga cccgtcgaac gggcaattga
     7501 cgctcgaagc cggatttctg gctttaaaag atatggtcgt cagcgtcatc ggcgaacacg
     7561 gattcaaata ttttccggtc gtcgcgtcat ttgccgtgct ggttctgatt tccaatctga
     7621 tggggctttt cccgctgttt atgtcgccga cggcgcacgt cagcgtgacg tttgcgctcg
     7681 gcatcgcttc gtttatttat tacaactaca tcggcatcag ggaaaacggc ttgataagct
     7741 atctggcgca ctttgccgga ccgaagctgc cgctcattat gatgctttta atcacgccgc
     7801 tgattttcgc catcgagctt ttcagtaata tgattcgccc gatgacgctc ggcattcgac
     7861 tgtttgcgaa tatgtatgcc gacgagcagg tggcgtcgat tatttcgggc ttgttcccgc
     7921 cgtttacgca atttctcgtg ccgattattc tgctgccgct cgcggtgttt atcgcgctgg
     7981 tgcagacgtt tgtgtttacg ctgctttcga tgatttacat cagcggcgtc tcgcacgcgc
     8041 cgcacgacca ccacgagcac gccggcaatg aacacgccca cacgggcgcg cacggcgaag
     8101 ttccggcggc gacggcgtcg gcttaaaatt tttgtgaacg attgatgaat gataaatcga
     8161 atcgtgttta tcattcgccg gttcacgatt cgctggttca cgattcgcaa ttcggctttg
     8221 acaaaccgga cggaaacaag gcgttttata gcagttttgt cagcacgctt acaggagcgg
     8281 cacgtctccc aagacagtgc gcggcatacc ggaagctgac gtaatcataa cccgcgccag
     8341 tgttttcaga agacgcgtcg aagtcaacaa aaaactaaat tcaggagata aaaatgaaca
     8401 aaattaaata tattttcttt gcggttctgg cgatggcttt gatgtcggtc acggctctgg
     8461 cgcagacggg cggcggcgcg gctgacaacg actcgaccgt aaacgcttac aaagcgattg
     8521 ccgccggtat cggtttc
//
LOCUS       DASWNE010000295         7261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_221391, whole genome shotgun sequence.
ACCESSION   DASWNE010000295 DASWNE010000000
VERSION     DASWNE010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7261
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221391"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VGB68_06115"
     CDS             complement(<1..530)
                     /locus_tag="VGB68_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048837.1"
                     /translation="MNKSIKAKNLSAQLQYQAAGNPPSTLPHSAISNAFPGLEMDFRN
                     IWKRILVGIELHESSNFVVGVDGDAPPEAEVLAGGYRLMRIEDVSFMVPVTGPKYAGG
                     ENVPLVDAFGDPRMPLEWSNALALIVRNFSGKPVNCSFESLEEPVKTVEIKLEVRRFF
                     DEADIDGEKTKLAVIA"
     gene            complement(523..1920)
                     /locus_tag="VGB68_06120"
     CDS             complement(523..1920)
                     /locus_tag="VGB68_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048838.1"
                     /translation="MAKKIAELRILPPFAIARLGSSKEPMDNYELKITDPIGVRQIVA
                     AETFLVDKKTGEITAKTPPFEVNFRDKTKKIRPIAPFLEVWARFDGDDNLVPLTADIL
                     SKAGLSPDDLKWSVRVANIKAFRRTGDINDKIEAQIENFSNHELQALNGYCNNFLEGK
                     SIPLGFVQYIKPTGDFPEIRLRFTPAAGYVYGPPPDPKADEDGENDPNIADAVYDPNK
                     GTWKGYCDPIDAKTDPIGVRKATNPAQIYAGKSTADGNQISYGYLDDECDGIVEVEME
                     IGGEKLSAYARIAAGPPTFAPDSMPVRTVGDELEQAMFGPDLDEPLTEQEISDVREIV
                     RRALETVRLMNTAQMNLPSKQRGVGMARMDYLDVSRALEPIVDLAVADSLAIRSRHER
                     VLLALESGSLAWFARILRNYDEVGDLSDEKRRKMPALMRSADGRHLALTRRQVNKVRA
                     AAEYYVELAKSINDE"
     gene            complement(1924..3357)
                     /locus_tag="VGB68_06125"
     CDS             complement(1924..3357)
                     /locus_tag="VGB68_06125"
                     /inference="COORDINATES: protein motif:HMM:NF024306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY0048839.1"
                     /translation="MATRLTKHLLWTETGSATAPVSRARGFLADEAAALPFTALETSS
                     EHPVEQQASLDIFTAPRDRAIYLMHVGAEVEHALMVQYLYAGYSLGGQHLTDPNHRRL
                     TQEWKAKILEIAREEMGHLVTVENMLTLIGGPLSFEREDYPIPEDLYPFPFELEPLTK
                     KSLGKYVLAEMPDAETIEKLGLTEEIEEIQKYVDGGSEKTKVNRVGILYAAILALFQP
                     PVQEKDPKPAPPSFISSSDIQSSSKRFQVNPDEWGLGYNDILIETAEDRPSAINALQL
                     IAAQGEGSTIGSLGGSHFGKFLEIYREFPDENGWQPSRNVARNPTTDSNAPADRYISN
                     PVANIWAGLLNLRYRMLLMYLIHSFQIEAPLKASGRTPRGLLVSWAFGEMYNIRSITE
                     ILMTLPMYKDGGELLAGPPFEMPYSLALAARNADRWRMHRDLLLASEQYVEKLLKAGG
                     GEYANYLKGLQSANESALAQVETLIGG"
     gene            4150..5292
                     /gene="thiO"
                     /locus_tag="VGB68_06130"
     CDS             4150..5292
                     /gene="thiO"
                     /locus_tag="VGB68_06130"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HEY0048840.1"
                     /translation="MNSEILIIGGGVIGLALARSLKKFGAGKITILERGKIGRESSFA
                     AAGILAPQAETDKPDDFFYFCNESNKLYPQLAAELFDETGIDIELERSGTLYLAFTDE
                     DVREINRRYAWQKGAQLAVEYLTAEEIRRSEPFISPDVREGLFFPNDRQVENRKLILA
                     LQKFAALNAIQIVENTEVKNLIVRNGRATGAETETGFFSAETIILTSGAWTSLVRLND
                     FDLPFKVRPIRGQMLSFQAAKRFFRHVIYSPRGYVVPRLDGRILTGATVEDVGFDKSV
                     TEAGIDFLLEHVLEIAPSLANLEINDKWSGLRPFAADALPVLGALPETENLFAATAHF
                     RNGILLAPMTAETLAGKIVKDVESPYLQIFKPVRSQESRVGSRQVG"
     gene            5320..6747
                     /locus_tag="VGB68_06135"
     CDS             5320..6747
                     /locus_tag="VGB68_06135"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY0048841.1"
                     /translation="MRKFLALSLIFSSLTFQIAAQSGRVTAGQPSDSISESEALTPKA
                     MYEEVNAYSKKKFDEFQQKKIPYSQALYQQTLREQRQLAARYAATLLARPPAPAGDDF
                     YYLGMLHWLAQNFDGADEALRKFFETKEASAENLQTSRSVMTVLAARRKNFDEAEKFL
                     SDYLRNEPVKLRERFNMESELAESYRAAKDYIKAAAHAEESYRAAKALLRDAASRTRA
                     LAEVLEAAMGLQEIYSESGDYKNAEATLGNLRKIAASVDSSAVYYYAIDQLVKFFIET
                     KRKPAALELYKASLNQTRKDFSNKALQDDVLQRLTRREKHYELLGETAPELAGIDHWF
                     PAADAKTLAGLRGKIVLLDFWATWCGPCIAAFPKLTGWHEAYQKDGFVILGLTRYYAV
                     TEGGKANRQIEADLVAKVKKTHRVPYDFVLAKDALNHHNYGADGIPTAVLIDRKGVIR
                     YIDSGTSAGREEELGKMIEKLLAEK"
     gene            6813..>7261
                     /locus_tag="VGB68_06140"
     CDS             6813..>7261
                     /locus_tag="VGB68_06140"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0048842.1"
                     /translation="MTKTQDLYDRIADVQNLAMKINGYRDSVAKYLRSLELDIKPDSL
                     VLDAGSGTGLVTLALYSAGYHPKKTFALDISYNSLTVAAQQFHEDKQVTAEDVEPVQG
                     NLLSLPFRDESFDVVLTCGALEYVPLDNGLQELARVLKKDGTLVLIPV"
BASE COUNT         1693 a         1887 c         1918 g         1763 t
ORIGIN      
        1 ttggctatta ccgccagctt cgttttttcg ccgtcgatgt ctgcttcgtc gaaaaaccgt
       61 cggacttcca gtttaatttc aacggtttta accggttctt ccagcgattc aaacgagcaa
      121 ttaaccggct ttccgctaaa attacggaca atcaaagcca gcgcgttcga ccattcgagc
      181 ggcattcgcg ggtcgccgaa agcatcgacc agaggcacat tttcgccgcc ggcgtatttc
      241 ggtcccgtga ccggcaccat aaacgaaacg tcttcgatgc gcatcagacg atagccgccg
      301 gcaagcacct ccgcttcggg cggcgcgtcc ccgtcgacgc cgacgacgaa attactcgat
      361 tcgtgcagct cgatgccgac cagaatccgt ttccagatat tgcgaaaatc catctccaag
      421 cccggaaaag cgttggaaat agccgaatgc ggaagcgtgc tgggcggatt gcccgccgcc
      481 tgatactgca actgcgccga aagatttttt gctttaattg atttattcat cgttgatact
      541 ctttgctaat tcaacataat attcggcggc ggcgcgaact ttattcacct gccgccgcgt
      601 cagagccaga tgccgaccgt cggcgctgcg catcagcgcc ggcatttttc gtcttttttc
      661 gtcggaaagg tcgccgactt catcataatt ccgcagaata cgcgcaaacc aggcgagcga
      721 gccgctttcc agcgccagca gaacgcgctc gtgacgcgag cgaatcgcca gcgaatcagc
      781 caccgccagg tcgacaatcg gctccagcgc acgactaacg tccagataat ccatccgcgc
      841 cataccgacg ccgcgctgct tcgagggcag attcatctgc gccgtattca tcagccgaac
      901 ggtttccagc gcgcgccgga cgatttcgcg aacgtccgat atttcctgct cggtcagagg
      961 ctcgtccaaa tcaggtccga acatcgcctg ttccagctcg tcgccgacgg ttcggacggg
     1021 catactgtcg ggcgcaaagg tcggcggtcc ggcggcaatc cgcgcgtatg cgctcagttt
     1081 ttcgccgccg atttccatct cgacttcgac gattccatca cactcgtcgt caagatagcc
     1141 gtaactgatt tgattgccgt cggcagtcga tttaccggcg taaatctgag cgggattggt
     1201 cgctttgcgg acgcctatcg ggtcggtttt ggcgtcgatc gggtcgcaat atcctttcca
     1261 cgttcccttg ttcgggtcgt aaaccgcatc cgcgatattc gggtcgtttt caccgtcttc
     1321 gtccgccttc gggtcgggcg gcggaccgta gacataaccg gcggcaggcg taaagcgcag
     1381 tcgaatctcc ggaaaatctc cggtcggttt tatatattga acaaagccga gcgggatgga
     1441 ttttccttcc agaaaattat tgcaatagcc gtttaacgct tgtaattcgt gattgctgaa
     1501 attttcgatt tgcgcttcta tcttgtcgtt gatgtcgccc gtgcggcgaa acgccttgat
     1561 gttggcgacc cggacgctcc atttcaaatc gtccggcgaa agtccggctt tcgataaaat
     1621 gtcggcggtt aagggaacga gattgtcgtc gccgtcaaag cgagcccaga cttcgagaaa
     1681 cggagcgata ggacgaattt ttttagtctt gtcgcgaaaa tttacttcga acggcggcgt
     1741 cttagccgta atctcgccgg ttttcttatc gaccagaaaa gtttcggcgg cgacaatctg
     1801 acggacgcca atcgggtctg tgattttcag ctcgtaatta tccatcggct ctttgctcga
     1861 accgagccgg gcgatggcaa acggcggaag aatgcgaagc tctgcgattt tttttgccat
     1921 attttaacct ccgattaaag tctcgacctg cgccagcgcg ctctcgtttg ccgactgcaa
     1981 acctttcaga taattcgcgt attcgccgcc gcccgccttc agcagttttt caacatactg
     2041 ctccgaagca agcagcaaat cgcgatgcat ccgccagcgg tcggcgttgc gcgcagccag
     2101 cgcgagcgaa tacggcatct cgaacggagg tccggcgaga agctctccgc cgtctttata
     2161 catcggcagc gtcatcagaa tttcggtgat cgagcgaata ttatacatct cgccgaacgc
     2221 ccacgaaacc agcaagccgc gcggcgttcg tcccgacgct tttaacggag cctctatctg
     2281 aaacgaatgt atcagataca tcagcagcat ccggtagcgc agatttaaaa gccccgccca
     2341 gatgttggcg accggattgc tgatatatct gtccgccggc gcgttggaat cggtagtcgg
     2401 gtttcgggcg acattgcgcg acggctgcca gccgttttcg tccggaaact cgcggtagat
     2461 ttccagaaat tttccgaagt gcgagccgcc gagcgagccg atggtcgagc cttcgccctg
     2521 tgcggcgatg agctgaagcg cattaatcgc cgacgggcgg tcttccgccg tttcaatcaa
     2581 tatatcgttg tagccgagtc cccattcgtc cggattgacc tgaaagcgct tcgagctcga
     2641 ttgaatgtcg gacgaagaga taaacgaagg cggcgcgggc ttcgggtctt tttcctgcac
     2701 gggcggctga aagagcgcga gaatcgcggc gtaaagaatc ccgacgcggt tgactttcgt
     2761 tttctcgctg ccgccgtcca catatttttg aatctcttca atttcttcgg tcagaccgag
     2821 tttttcgata gtttccgcgt cgggcatctc ggctaaaacg tatttgccca gagatttttt
     2881 ggtcagaggc tccagctcaa acggaaacgg atacaaatct tcgggaatcg ggtaatcctc
     2941 gcgctcgaac gaaagcggac cgccgattaa agtcagcata ttttcgaccg tcacgagatg
     3001 tcccatttcc tcgcgcgcga tttccagaat tttcgccttc cattcctgcg tcaatcgacg
     3061 gtgattcggg tcggtcagat gctgcccgcc gagcgaataa ccggcataca ggtactgcac
     3121 catcaaagcg tgttcgactt cggcgccgac gtgcatcaga taaatcgcgc ggtcgcgcgg
     3181 cgcggtaaat atatcgagac tcgcttgctg ctcgaccgga tgctcggaag atgtttccag
     3241 cgccgtaaaa ggcagagcag ccgcttcgtc ggctaaaaac ccgcgcgccc gggaaacggg
     3301 cgctgtagcc gaaccggttt ccgtccacaa taaatgtttg gttaatcttg ttgccacaat
     3361 tttcctcgga tattcccgtt tagggttttc ttaaataaaa aagccgaaat ccctttcagg
     3421 ataatcagct ttttttaaca ttctgttttt cgtaatttag atttgtgacg ctaaaatcgg
     3481 gctgcccgtt ccgggttcgg caaaataatc gccgccagcc tccgcaaaag ccggcgaatt
     3541 cccgtatttt actagcattg catgggcggt tcgacataac gatagatact tgaggcactg
     3601 aatttcacaa ttcgagcctt ttatttttct ctttttctta agcttatctt ttaccgaaac
     3661 gctctttaat tttccccttc aaaacatttt cggtaaagct cttatgtcgt cggttgatag
     3721 aaattatcac aggttaattt taactgtcaa tgatttttta attgcgcgcg gcggaaaacg
     3781 atttttccgg ctcagcctcc ggaagaagcg aaaacgaaaa gaaatgaaaa ggctcgggat
     3841 acgtgccgtc ccgagccttt tcatttttcc aaggagtaag agaatatgaa tcaaagaatc
     3901 gtcgccgacc ttcgctgctg tatgaaagac ttattcgcga ttagtttcca gaaactaatg
     3961 aaaacaattt tttgctgaaa tttatttcgg tatcgctcct ggattttatt cgctcgccaa
     4021 accctccggc aagtcgcaga ggctgccgta tgatagtatg ttttattgat ttgtcatacg
     4081 ttttatggtc agtaggattg cttttgcaat tataaattta tacgggtaag ggacagcgaa
     4141 attatcttga tgaattcgga aatcttaatc atcggcggcg gcgttatcgg cttggcgctg
     4201 gcgcgcagct taaaaaaatt cggcgcgggc aaaatcacga ttctcgaacg cggaaaaatc
     4261 gggcgcgaat cttctttcgc cgccgccgga attctcgcgc cgcaggcgga aaccgataaa
     4321 ccggacgatt ttttttattt ttgtaatgaa tcaaacaaac tttacccgca gcttgccgca
     4381 gaattgtttg atgaaaccgg catcgatatt gagcttgagc gaagcgggac gctttatctg
     4441 gcgtttaccg acgaggacgt tcgcgaaatc aaccgccgct acgcctggca gaaaggcgcg
     4501 cagctggcgg tcgaatacct gacggcggag gaaattcgcc gcagcgagcc gtttatttcg
     4561 cccgacgtgc gcgagggctt gttttttccg aacgaccggc aggtcgaaaa tcgcaagctg
     4621 attctggctc tgcaaaaatt tgccgcgctc aacgcgattc aaatcgtcga aaacacggaa
     4681 gttaaaaatt taatcgtccg taacggcaga gcgaccggcg cggaaaccga aaccggattt
     4741 ttttcggcgg agacgattat tttaacgagc ggcgcgtgga cgtcgctcgt caggctgaac
     4801 gattttgatt tgccttttaa ggtgcgaccg attcgcgggc aaatgctgag ctttcaagcc
     4861 gcgaaacgtt tttttcgaca cgtcatttac agcccgcgcg gttacgtcgt cccgcgtctg
     4921 gacgggcgaa ttctgaccgg cgcgaccgtc gaagacgtcg gcttcgacaa aagcgtcacc
     4981 gaagcgggaa tcgatttttt gctcgaacat gttttggaaa tcgcgccgag ccttgctaat
     5041 ttagagatta acgataaatg gtccggcttg cgtcctttcg cggcggacgc gctgcccgtc
     5101 ttgggcgcgc tgcccgaaac ggaaaatctt tttgcggcga cggcgcattt ccgcaacggc
     5161 atactgctgg ctccgatgac ggcggaaacg cttgcaggca aaatagttaa agatgtagaa
     5221 tcaccttatt tgcagatttt caaacccgtt agaagtcagg agtcgagagt cggaagccgg
     5281 caggtcggtt gaatatttcc gcgctcggct gcaaaatcta tgcgtaagtt tttagcttta
     5341 agcttaattt tttcatctct gacttttcag attgccgcgc agtccgggcg cgttacggca
     5401 ggtcagccgt ctgattcgat ttccgaaagc gaagcgttga cgccgaaagc gatgtatgaa
     5461 gaagtaaacg cttattcaaa aaagaaattc gacgagtttc agcaaaagaa aattccttac
     5521 agccaggcgc tttaccagca gacgctgcgc gagcagagac agcttgcagc ccgttacgcc
     5581 gctacgctgc tggcgcgccc tcctgcgccg gcgggcgacg acttttatta tctcgggatg
     5641 ctgcactggc tggcgcagaa tttcgacggc gcggacgaag ctttaagaaa gttttttgaa
     5701 acgaaggaag cgagcgccga aaatctgcaa acttcgcgtt cggtaatgac ggtcctcgcc
     5761 gcgcgccgga aaaattttga cgaagccgaa aaatttctga gcgattatct gaggaatgaa
     5821 ccggtcaagc tgcgcgagcg tttcaatatg gaaagcgagc tggcggaaag ctatcgcgcc
     5881 gcgaaagatt acattaaagc cgcagctcac gccgaagaat cttatcgcgc ggcgaaagct
     5941 ttattgcggg atgccgcttc gcgcactcgc gcgcttgccg aagttctcga agcggcgatg
     6001 gggcttcagg aaatttacag cgagagcggc gattataaaa acgccgaagc gacgctcggc
     6061 aatttgcgga aaatcgccgc ttccgtcgat tcgtccgccg tttattatta cgcgattgac
     6121 cagttggtta aattttttat cgagaccaaa cgcaagccgg cggcgctgga attgtataaa
     6181 gcctcgctga atcaaaccag aaaggatttt tcgaataaag cattacagga cgacgttttg
     6241 cagcgcttga ccagacgcga aaagcattat gaactgctcg gcgaaactgc gccggagctt
     6301 gccggaatcg accactggtt tcccgccgcg gatgcgaaaa cgcttgccgg gctgcgcgga
     6361 aaaatcgttt tactggattt ctgggcgacg tggtgcggac cttgcatcgc cgcgtttccg
     6421 aagctgaccg ggtggcacga agcgtatcag aaagacggtt ttgtcattct cggcttgacg
     6481 cgttattacg ccgtcaccga aggcggcaag gcaaaccggc aaatcgaagc cgatttggtc
     6541 gcgaaggtta aaaagacgca tcgcgtgccg tatgatttcg tgcttgccaa agacgctctg
     6601 aatcatcaca attacggcgc ggacgggatt ccgaccgccg tgctgattga ccgcaagggc
     6661 gtgattcggt acattgacag cggaaccagc gccggcagag aagaagaatt aggaaagatg
     6721 attgaaaaac ttttagcgga aaagtaaagt aacaaagcga ataaaaggat tttcgctttt
     6781 gctttgaaaa ttttaaaatg gctcaagaaa agatgactaa aacgcaagac ctgtatgacc
     6841 gcattgccga cgtgcagaat ctggcgatga agataaacgg ttaccgtgat tcggtggcga
     6901 aatatctgcg ctcgctcgaa ttggacatca agccggattc gctcgttctg gatgccggtt
     6961 cgggaacggg cttggtcact ctcgcgttat acagcgccgg ttatcacccg aaaaaaacgt
     7021 ttgcgctgga tatttcctac aattcgctga cggtcgccgc gcagcagttt cacgaggaca
     7081 agcaggtgac ggcggaagat gtcgagccgg ttcagggaaa tctgctttcg ctgcccttta
     7141 gagatgaatc tttcgatgtg gttttaacct gcggagcgct cgaatacgtg ccgctcgata
     7201 acggcttgca ggaactggcg cgggttttga aaaaagacgg aaccctggtt ttgattcccg
     7261 t
//
LOCUS       DASWNE010000296         1623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_9929, whole genome shotgun sequence.
ACCESSION   DASWNE010000296 DASWNE010000000
VERSION     DASWNE010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9929"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VGB68_06145"
     CDS             <1..327
                     /locus_tag="VGB68_06145"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEY0048843.1"
                     /translation="VFYPALTSHPQYEIAKRQMNGFGGMISFDVGGVEEGKRLVNNVE
                     VCTLATSLGGVETILQHSASMTHATLSGEQRLKAGVTDGLIRLSVGIEDAQDLISDLE
                     SALGKI"
     gene            339..1049
                     /locus_tag="VGB68_06150"
     CDS             339..1049
                     /locus_tag="VGB68_06150"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048844.1"
                     /translation="MPIKPETAGRAELVEKIKEEKPFPYIVQNLERTFGEPALGRKSD
                     PLAMLVNIILSQATSDANSRRTFENLKNRFEDWDAVSAADENEIADAIRLGGLANQKA
                     RVIKNLLRQIKAIRGTLSLKFVEKMTDAEARDFLSQFRGIGPKTVACTLLFACHKEVF
                     PLDTHIFRVLKRMGILPEKISDARAHALLDELVPEGKFYSLHVNLIRLGRKICRPREP
                     LCETCPLVEYCDYGQTKI"
     gene            1062..>1623
                     /locus_tag="VGB68_06155"
     CDS             1062..>1623
                     /locus_tag="VGB68_06155"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEY0048845.1"
                     /translation="MRRFFSEFSATFFLLAIIVGSGIMGERLAGGNAAIALLANTLAT
                     GAGLVFLILTFGEISGAHMNPVVSLVELRRGNLPVKDFFGYAAAQMAGAISGVGAANL
                     MFDLPIFFFSAKVRSGAAQFFSEFLATFGLLAVILATTKFRPQMTPFAVAAYIASAYW
                     FTASTSFANPAVTVARSLSDTFAGIRP"
BASE COUNT          370 a          408 c          452 g          393 t
ORIGIN      
        1 gttttttacc cggctttgac ttctcatccg caatacgaaa tcgcaaagcg acagatgaac
       61 ggcttcggcg gaatgatttc ttttgatgtg ggcggcgtcg aagaaggaaa acgtctggtc
      121 aataatgtcg aagtctgtac gctggcaaca tcgctcggcg gcgtggaaac gattcttcag
      181 cattcggctt cgatgacgca cgcgacgctc tccggcgagc aaagattaaa agccggagtc
      241 accgacggct tgattcggct gtcggtcggc atcgaagacg cgcaggattt gatttccgat
      301 ttggaaagcg cgctcggtaa aatttgattg attaatagat gcccattaag cccgaaaccg
      361 caggacgcgc cgaactcgtc gaaaaaataa aggaagaaaa gcctttcccg tacatcgtcc
      421 aaaatctgga gcgaaccttc ggcgagcccg cgctcgggcg aaagagcgac ccgctggcga
      481 tgctcgtcaa tattattttg tcgcaggcga ccagcgacgc aaacagtcgc aggactttcg
      541 agaacttgaa aaatcgcttt gaagattggg acgcagtttc ggcggctgac gaaaacgaaa
      601 tcgccgacgc catcaggctc ggcggtctgg caaatcaaaa ggcgagagtc atcaaaaatc
      661 ttttgcggca aatcaaggca atacgcggca cgctctcgct taaattcgtc gaaaaaatga
      721 cggacgcgga agcacgggat tttctgtcgc agtttcgcgg aatcggtccg aaaaccgtcg
      781 cctgcacatt gctgttcgcc tgtcacaaag aagtttttcc gctcgacacg catatttttc
      841 gcgttttgaa acggatggga attttgcccg aaaaaatttc cgacgcccgt gctcacgcgc
      901 ttctggacga gcttgtgccg gaagggaaat tttattcgct gcacgtcaat ctgattcggc
      961 tcggcagaaa aatctgccgc ccgcgcgagc ctttgtgcga aacgtgtccg ctcgtcgaat
     1021 attgcgatta cgggcagacg aaaatctaaa atccgattca gatgagaaga ttcttttccg
     1081 aattctcggc gacttttttt ctgctggcga taatcgtcgg ttcggggata atgggcgaaa
     1141 gactggcagg cggaaatgcc gccatagcat tgctggctaa cactctggcg acgggcgcgg
     1201 gacttgtttt tttgattctg acttttggcg agatttccgg cgcgcatatg aatccggttg
     1261 tcagtttagt tgagctgcgg cgcgggaatt tgccggtaaa ggatttcttc ggttacgcgg
     1321 cggcgcagat ggcgggcgcg attagcggag tcggcgcggc aaatctgatg ttcgatttgc
     1381 cgattttctt tttttcggcg aaagtgcgca gcggagcagc gcaatttttc agcgaatttc
     1441 tggcgacgtt cggcttgctc gccgtcattc tggcgactac caaatttcga ccgcaaatga
     1501 cgccgtttgc ggtcgccgcc tatatcgctt cggcttactg gttcacggct tccacctctt
     1561 tcgccaatcc tgccgtcacg gttgcgcgaa gcttgagcga cactttcgcg ggaattcgtc
     1621 cgg
//
LOCUS       DASWNE010000297         2502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_396189, whole genome shotgun sequence.
ACCESSION   DASWNE010000297 DASWNE010000000
VERSION     DASWNE010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396189"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..573)
                     /locus_tag="VGB68_06160"
     CDS             complement(106..573)
                     /locus_tag="VGB68_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048846.1"
                     /translation="MKKIFLRLAVLSILLFALFQIREQSVLAAYCWEGVYAGWNNCDN
                     NYSATINAHWNSPNTCNSQATAACANDPNPSSCYTTQYNNCIQASQNTYDNRGMDYGS
                     CISGVFSSNCVEQLDYCDQARSRRDGCILQYSGTDDADAYYACWSASGIHLCE"
     gene            complement(1647..2042)
                     /locus_tag="VGB68_06165"
     CDS             complement(1647..2042)
                     /locus_tag="VGB68_06165"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0048847.1"
                     /translation="MQPEKTILYIDYSSDNAEVVKFFLNQSGYEVETCRTGEAGLDLA
                     RQNDFALIMTEFRLPDMHGTDFCREFRKFNARIPLMFFSASVQKWEKEEGLASGAQAY
                     LIKPDDLDKITDTVGSLINRNHQFTTARK"
     gene            complement(2255..>2502)
                     /locus_tag="VGB68_06170"
     CDS             complement(2255..>2502)
                     /locus_tag="VGB68_06170"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEY0048848.1"
                     /translation="EILGRKVYRLLADIPGGVDLINVFRRSADIPQHTEDILAAKPKA
                     VWFQSGIRNDSVANELAQNGIKVVQERCIMVEHRVSL"
BASE COUNT          652 a          581 c          595 g          674 t
ORIGIN      
        1 ataatcgtgc ggataacgcg aaaatccagg caaaaggcgc cgcccgaata ttcgggcggc
       61 gccttttgcc tggattttcg cgttatccgc acgattattt acgcgctatt cgcacaaatg
      121 tattccgctc gccgaccagc aggcgtaata agcgtcagca tcgtcagtgc cggaatactg
      181 caatatgcag ccgtcgcgcc gcgagcgcgc ctggtcgcag taatcgagct gctcgacgca
      241 gttcgagctg aaaacgccgg aaatgcacga accgtaatcc attccgcgat tatcgtaggt
      301 gttctgcgac gcctgaatac aattgttgta ttgagtggtg taacacgatg acggattcgg
      361 gtcgtttgcg cacgccgccg tcgcctgcga attacaggtg ttcggcgaat tccaatgcgc
      421 gttgatggtc gcggaataat tgttgtcgca attattccag ccggcataga cgccttccca
      481 gcaataagcc gccagcacgg attgttctct gatttgaaac agcgcaaaca gcaaaattga
      541 taaaacggcg agccgtaaga aaatcttttt catatttttc cttttctgat ggagtgtgta
      601 aaccgcgccg agtcgagcga tttactttcg atacgtgtag gctgcgcaga ctttaactgc
      661 tctaaatcgg cgcgttgaat tcgagtgtcg caggcattaa accgcgtatc cggtttgatt
      721 tttaatcgct cgaattatcc aatgggctat cagatacttt taaaggcgtc gccgctccgg
      781 gcacgctcaa aattcgactg ccgaatttcc ggctttccgc tttgcacgca ttatttccgg
      841 tcgaaaaatc agctcggttt ttcagaacag ggcggacaag atgcgaatcg gatagacggc
      901 gtcttacgaa caaactgttt cggaactgcg ccaaaatggc tcgaaaaatg atgacagtcg
      961 gagttccggc ttttcttttg actcggtctc ggagaaaagc gagtcgcttc aaatcttcgg
     1021 ctttaacaaa aaaagccggt tatcacacga cggacaatcg actttcgata aaatttttct
     1081 gagttttaga ttgttttccg gcataaccgc gcgatgtttc cggagtttcg cgcacgctcc
     1141 gtgatttgtc gaatatctca acgcagaaac aggttaaatc gcaaacttgt cctgacgttt
     1201 gagcagatga tttttgattt gatgactttg ccgcgtcatc cgtttttatg tttacagaaa
     1261 aattgacctt gtaatgcttt tttacattac aaatattcga cgccgattac cgggaaaatt
     1321 tagcacaggg caaaatccgg tgtcaatgtt ttttggtttg gaaaaaataa agattcggcg
     1381 gcggcgcgaa taaatatagg cgagataagc cgggacgctt tccctaactt caaatcccac
     1441 gataaacgag tgatttttcg gctggcttct accctgactt atcccgcctt ttatagaaca
     1501 ataaattaaa tttacctctt ctaagtcgaa tggttctgtt agatacttta cataaacgaa
     1561 aaaaaattta tccgcggcag cgtttcccga attttcgata agcatccgac gggcgagata
     1621 aaatcagttc cggtcaaggc gaaaaactat ttgcgggcgg ttgtaaattg atggttccgg
     1681 ttgataagag aaccgacggt atcggtgatt ttgtccaaat cgtcgggttt gatgagatac
     1741 gcctgcgcgc ccgaagccaa gccttcttcc ttttcccact tttgaaccga ggcggaaaaa
     1801 aacatcagcg gaatccgggc gttgaatttg cgaaattcgc ggcaaaaatc cgtgccgtgc
     1861 atatcgggca ggcgaaattc ggtcataatc agcgcgaagt cgttctgacg ggctaaatca
     1921 agcccggctt cgcccgtccg gcacgtttcg acttcgtaac ccgattgatt taagaaaaat
     1981 ttgacgactt cggcattatc gctgctgtaa tcgatgtaaa gaattgtttt ttccggttgc
     2041 acggcggctt tcctcctttg aaagcttggg aaagattgcc tgataaattc ctgagacgga
     2101 agcagaatca aacaaaattc caaatcaaat tttcccggcg ggctttgatt cagcgtggga
     2161 taaattaatt aaaattgtag gttttgccgg acttttagat agcaaaaccg aatcaataaa
     2221 aggagagtcg caaattttgc ggtttatcga ttcattaaag cgaaactctg tgttcgacca
     2281 ttatacaacg ttcctggacg actttgattc cgttttgcgc caattcgttg gcaacggaat
     2341 cattgcggat gcccgattga aaccagacgg ctttcggttt cgccgccaga atatcttcgg
     2401 tatgctgcgg aatatccgcc gagcgccgga aaacattgat taaatcgacg ccgcccggaa
     2461 tgtccgccaa aagtcgataa actttccgcc cgagaatttc cg
//
LOCUS       DASWNE010000298         4176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_9943, whole genome shotgun sequence.
ACCESSION   DASWNE010000298 DASWNE010000000
VERSION     DASWNE010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9943"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VGB68_06175"
     CDS             <1..257
                     /locus_tag="VGB68_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048849.1"
                     /translation="NPKYPRVAEDFAGTFETLKNIKADVFLSSHGQFFDLNKKAEKLR
                     AGAKPNPFIDPAGYQRFVARMRKAFEVKLKAERESKKVKA"
     gene            complement(362..1876)
                     /locus_tag="VGB68_06180"
     CDS             complement(362..1876)
                     /locus_tag="VGB68_06180"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY0048850.1"
                     /translation="MKDSEFYSYKFAKTLNLKTGAALLLFCLLTVSFSLQASAQTSAS
                     APADAAEQRGRAFVEAINSNNRATWRKFISENFAKSSFERLPLEARLNNFARIYDQTR
                     GLNLQNARRTKPNEASLAVRSSLTGIGMELMFLVDEQSPFYLSSLGIRAQQTPPGKKL
                     SDREMVEQLDDFLKKLSAADVFSGAVLLARGDGVLFEKAYGEANKDFKAPNNVNTKFN
                     LGSMNKMFTSVAVAQLVEAGKLSFDDSLSKFMPDFPDREAAEKIKIKHLLSHTSGLGN
                     YFNRTFMDASRARFRTVDDFLELAKTEKMQFEPGTKWQYSNTGMLVLGRVIEKASGQN
                     YFDYIRENIYKKAGMTSSDSYDLDSVNPNLATGYEKDFTEQGVRFRNNIFIHVIRGGP
                     AGGGYSTVGDLLKFARALQSGKLVGKEYVKLLTSPKPELNSPEYGYGFGMGADPNTIG
                     HSGGFPGVSSELIIFTNSEYTAVVLSNYGGGSQPVTTKIRGLVQSCADCQTAKN"
     gene            complement(2161..>4176)
                     /locus_tag="VGB68_06185"
     CDS             complement(2161..>4176)
                     /locus_tag="VGB68_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY0048851.1"
                     /translation="ASSAGSRYFGKTQAPKDNVLRYISGSEPESLDPHVSSGQPEARV
                     YMAIYDGLVEYHPKDMHPIPALAESWEISPNVDEFIFRLRDNAKWSDGTPITARDFVY
                     SFRRGFAPETASRTASLGYFIKYAEAFNGGQVFVKKNGEFLLAKDFAEDVAAAAKTND
                     SPGQKTSPARTSFGAETAFRKFIKSPDRLVLDGDEKKRAKQIEANPMLRAALAESVEF
                     VPVKAEDIGVEMLDDYTLRITLRQPAPFFLGLLAHQFFRLVPQKAIEKHGKDWTRAKN
                     IVTCGAFKIKKIQPYDAYIFEKDPNYWDAANVRLDGIEFYPIEEQTTILNLYKAGNID
                     AMLNHIVPSSWIDVVREYKDEYMNLPENATAYYSMNITKPPFDNPKVRQAFSLAINRE
                     ILAQFRKTTKPLYFQTPSGIFPDYDKAMDKVGEEVRKERGISPENWAKQRKQFDPEFA
                     RKLLTEAGFPVRQNGESFSAPAFPADKVAISYNTLESNRQIAEFIQNQWKQNLGITIP
                     LKNMEFKTFLPYRNSLEYEGFAQSLWGGDYMDPFTFLGLHYGQQNDGGSGFSDPRYDR
                     MLDEANAELDAQKRYEKLARAEYYLMEQLPSVPLTIAATNWMKKPYVKGMYPNPGTLH
                     PWKFVYIERDPARWDRDVENIMRDKDALVEEQIKNLEKTQIQINSED"
BASE COUNT          978 a         1068 c         1041 g         1089 t
ORIGIN      
        1 ataatccgaa atatccgcgc gtcgccgaag atttcgccgg aacattcgaa acgctcaaaa
       61 acattaaggc ggacgtcttt ttgtcatcac acggacagtt ttttgattta aacaaaaaag
      121 ccgaaaaact tcgcgcgggc gctaaaccga atccgtttat cgacccggca ggctatcaga
      181 gatttgtcgc ccgaatgaga aaagccttcg aggttaaact gaaagccgaa agagaaagca
      241 aaaaagtgaa agcataaatt tttccgtcag gtaaaaagcg ttgaatcaaa aatcggggcg
      301 gctgattttg gtaattaaaa tcagccgccc cgattttttg attatctgtt tgtagcccgc
      361 ttcaattttt cgccgtctga caatccgcgc acgactgcac gagcccgcga atttttgtcg
      421 tgacaggctg ggagccgccg ccgtaattgg atagcacgac cgccgtatat tctgaattgg
      481 taaatataat cagctcggag ctaacgcccg gaaagccgcc gctgtgcccg atcgtgttcg
      541 ggtctgcgcc cataccgaat ccgtaaccgt actcgggcga attaagttcc ggtttcggcg
      601 aggtcagcag tttaacgtat tccttcccga ccagttttcc gctttgcagg gctctggcaa
      661 atttcagcaa atcgccgacg gtcgagtaac cgccgcccgc cggaccgccg cgaatcacgt
      721 gtataaaaat attgttgcgg aaacgaacgc cctgctcggt gaaatctttt tcgtaaccgg
      781 ttgccagatt tggatttacg ctgtccaggt cgtaggaatc ggagctggtc attccggctt
      841 ttttgtaaat gttttcgcgg atgtaatcga aatagttctg ccccgaagcc ttttcgatga
      901 ctctgcccag aacaagcatt ccggtattgc tgtattgcca tttcgtgccc ggctcaaact
      961 gcattttttc cgttttggcg agttcgagaa aatcgtcgac cgtgcggaaa cgggcgcgcg
     1021 aggcgtccat aaatgtgcgg ttaaaataat ttcccaaacc cgacgtgtgc gagagcagat
     1081 gcttgatttt tatcttttcc gccgcttccc tgtccggaaa atccggcata aatttcgaga
     1141 gcgaatcgtc gaacgagagc tttccggctt cgaccaactg agcgacggca accgacgtga
     1201 acattttatt catcgagccg agattgaatt tcgtgttcac gttgttcggc gctttaaaat
     1261 ccttgttcgc ctcgccgtaa gctttttcaa acaaaacgcc gtcgccgcgc gccagcagaa
     1321 ccgcgccgga aaaaacgtca gccgcgctca gctttttcag aaaatcgtcg agctgttcga
     1381 ccatttcccg gtcagataat ttcttgcccg gcggcgtttg ctgcgcgcga atgccgagtg
     1441 agctgagata aaacggcgac tgctcgtcga ccagaaacat cagctccata ccaattcccg
     1501 tcaagctgct cctgacggcg agcgaagctt cgttcggttt tgtacggcgc gcgttttgca
     1561 gattgagccc gcgagtctgg tcgtaaattc gcgcgaaatt atttaatcgc gcctcaagcg
     1621 ggagcctttc aaacgaagat ttggcaaaat tttcgctgat aaattttcgc cacgtcgcgc
     1681 gattattcga attaatggct tcgacaaaag cgcgcccgcg ctgttcggcg gcatcggctg
     1741 gcgcagacgc cgatgtttgc gcagaagctt gcagggaaaa gcttacggtc aataaacaaa
     1801 acaacaatag cgccgcgccg gttttcagat tcaaggtttt tgcgaattta tacgaataaa
     1861 attcagagtc tttcataatt gttaacgatt cctttttgac tggattgctg aagcagtcat
     1921 aaacattacg agccgtctct gactttagtt tcatctaaac cgcgacaaat taaccggcag
     1981 gcgctgagaa tggaaatacc ggcgggcgtt aaaggcaatt ccgtaattag agttggttta
     2041 aagtgaaaaa ctgaaaaagt gaaaaagtga aaaagtgaaa agatgaaaaa gaattgttga
     2101 cttcgctctg actttccttt tcaccttttc actttttcac ttttggcatc gcggcgctcg
     2161 tcaatcctcg gaattaattt gaatttgcgt tttttccaga ttttttatct gttcttcgac
     2221 gagcgcatct ttgtcccgca ttatattttc gacatctctg tcccatctgg ctgggtcgcg
     2281 ctcgatatag acgaatttcc atgggtgaag cgttcccgga ttcggataca tacctttgac
     2341 ataaggcttc ttcatccagt tggtcgcggc tatcgtcagc ggcaccgaag gcaactgctc
     2401 catcaggtaa tattcggcgc gagccagttt ctcgtagcgt ttctgcgcgt cgagctccgc
     2461 gtttgcctcg tccagcatcc tgtcgtatct cgggtcggaa aagcccgagc cgccgtcgtt
     2521 ctgctgaccg taatgcaagc ccagaaacgt aaacgggtcc atataatcgc cgccccacaa
     2581 gctctgcgca aagccttcgt attcgagcga attccggtaa ggcagaaacg ttttgaactc
     2641 catattttta agcggaatcg tgatgccgag attttgtttc cattgattct ggataaactc
     2701 ggcgatttgc cggttcgatt cgagcgtatt gtaggaaata gcgaccttgt cagccggaaa
     2761 cgccggcgca gagaagcttt cgccgttttg cctgaccggg aaaccggctt cggtcaacag
     2821 cttgcgggcg aattcggggt cgaactgctt tctttgtttc gcccagtttt ccggcgagat
     2881 gccgcgctct ttcctgacct cctcgccgac tttgtccatc gctttatcgt aatcgggaaa
     2941 aattccggac ggcgtctgaa agtaaagcgg tttggtcgtt ttgcgaaatt gcgcgagaat
     3001 ttcgcggtta atcgccagac tgaaagcctg ccgcactttc ggattatcaa acggcggctt
     3061 cgtgatattc atactgtaat aagccgtcgc gttttccggc aggttcatat actcgtcttt
     3121 atactcccga acgacgtcaa tccacgaaga cggaacgatg tgattgagca tcgcgtcgat
     3181 gttaccggct ttatacaagt tgagaatcgt cgtctgctct tcgattggat aaaattcgat
     3241 gccgtccagc cgaacgttcg ccgcgtccca gtaattcggg tctttttcaa aaatataagc
     3301 gtcataaggc tgaatctttt tgattttaaa cgcgccgcag gtcacgatgt ttttcgcgcg
     3361 cgtccaatct ttgccgtgtt tttcaatcgc cttttgcgga acgagccgga aaaactgatg
     3421 cgccagaagc ccgagaaaaa aaggcgcggg ctggcgcaga gtgattctca aggtgtaatc
     3481 gtcgagcatt tcgacgccga tgtcttcggc tttaaccgga acaaactcga ccgattccgc
     3541 cagcgccgct ctcagcatcg ggttagcttc aatctgtttg gcgcgttttt tttcgtcgcc
     3601 gtccagaacc aggcggtcgg gcgatttgat gaatttgcga aacgccgttt ccgcgccgaa
     3661 agacgttcga gccggagaag ttttttgtcc gggcgaatca tttgttttcg cggcggcggc
     3721 gacatcttcg gcaaaatcct ttgccagcag aaattcgccg ttctttttta cgaaaacctg
     3781 accgccgtta aaagcttcgg cgtatttgat aaaatagccg aggctcgccg tgcgcgaagc
     3841 ggtttcgggc gcaaatccgc gccgaaagct gtaaacgaaa tcgcgcgccg taatcggcgt
     3901 cccgtcgctc catttcgcgt tatcgcgcag gcgaaaaata aattcatcga cattcggact
     3961 gatttcccag ctttcggcga gcgccggaat cgggtgcata tctttcggat gatattcgac
     4021 caagccgtcg taaatcgcca tataaacgcg cgcttccggc tgaccgctgg agacgtgcgg
     4081 gtcgagcgat tccggctcgg agccggagat ataacgcaga acattatctt tcggagcctg
     4141 cgtttttccg aaataccgac tgcccgcgga actcgc
//
LOCUS       DASWNE010000299         3040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_679472, whole genome shotgun sequence.
ACCESSION   DASWNE010000299 DASWNE010000000
VERSION     DASWNE010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679472"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VGB68_06190"
     CDS             <1..184
                     /locus_tag="VGB68_06190"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0048852.1"
                     /translation="EISVEIYPEEANLYDSIGEFYNSLGNKEKAIEFYTKALEINPKY
                     PNAANAKELINKLKEQ"
     gene            complement(407..>3040)
                     /locus_tag="VGB68_06195"
     CDS             complement(407..>3040)
                     /locus_tag="VGB68_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048853.1"
                     /translation="RDPDDSFFARQKGTFFFFSQEWRKDLRYPTLTSTVPSLAQRQGV
                     FTVPICLQATAGTCTTILPAGTPLSTLRPINTVAQQYLTAIYNKLPAPNSGANSLLFP
                     TLNKSDFRQEILKIDHNFTKNWSMYYRFQQDAIPTIEANSLFSSGSGLPGVSTTATDS
                     PGKAHTVQSTYVLNPRMIIEARYTYSYGAILSENIGLLALTNSTINVPLPFANTRDRI
                     PTISGNGFSALQGFGPYDNFSDKHNWSGSFTWIMGDHTMKFGGGYSLYRKNENAIAGN
                     NEGIFSGFNTPGATTALPAPGCIPAQQPCGTQTSQQQFANFLLGTNVSFTQASFDYTA
                     DLRQKTIEGFAQDEWRVRKNLTLYMGVRYSFFGSPWDKNGRLTNFIPEMFNQSAAPLV
                     TGAGNRVAGAGKNFCNGLIANTQNYTTGPAAFNCTPTPSPWGKFVIDAPKTDFAPRVG
                     LAWDPFGKGETSIRTGYGIYHDQVLNGNQLQQIGLNPPYQQTCTVTGVNLADPVPGGN
                     CTVASSLAAPNIRGVQADWKTPYMQHWSLDLQQQLTSKTLVTIGYYGSKGTNLIGAYE
                     LNALPPGYAISLGATGCAVGASTTPTAPCQVAGTAFFTGAQTRILDQLRPYRGYQSIN
                     MVQPRYDSNYHSMQFSAQHRFSGASQVNFAYTWSKNLTNNQTDRSTAPQNSYDIESEW
                     GRASLDRRHIFSMNYVYEVPFFNNRNDFAGKVLGGWQVSGIVTYSTGLPLTPVINSAL
                     GAFDAAGLGIIPAIIAGTRPNVICDPNQGGAGTQQQWFNTACFTPNPASNATNIPNIV
                     GSAGRGIIEGPSRRRVDFTLTKNIRFNETMRLQLRGEAFNIFNWVSYNTVDVRVNAST
                     FGQVTGVRDPRTLQFGAKFYW"
BASE COUNT          743 a          736 c          849 g          712 t
ORIGIN      
        1 agaaataagc gttgaaattt atcctgaaga agcaaatctc tatgacagca tcggcgaatt
       61 ttataacagt ttgggaaata aggaaaaggc aatcgaattt tatacgaagg cacttgaaat
      121 taaccccaaa tatcctaatg cggcgaatgc aaaagagtta ataaataaat taaaggaaca
      181 gtaatcctaa aaataaaata agacgggata attgaatttt gcacaaaacc agccgcaggt
      241 tgctcaaata gtattcgcta gtggaaagac tggaaaatcg gtgaaaaagc gaaattaaca
      301 gtgtctcgct ttcgccgtca aaacagcaaa gtttttaact ttcaaaaaac aggaagcctg
      361 cctttgccga ttttggcaaa ggcaggcttc ctgtggcaat taaatattac cagtagaatt
      421 tcgcgccgaa ctgcaatgtg cgcgggtcgc ggacgccggt cacctgaccg aacgtggagg
      481 cgtttacgcg gacatcgaca gtattgtagc tgacccagtt aaaaatattg aacgcttcgc
      541 cgcgaagctg caagcgcatc gtttcgttga aacgaatatt ttttgtcagc gtgaaatcaa
      601 cgcgccgtct cgaaggtcct tcgataatac cgcgtccagc cgaaccgaca atgttcggaa
      661 tgttggtcgc gttactcgcc ggattgggcg tgaaacaggc tgtgttaaac cattgctgtt
      721 gtgttcccgc gccgccttga ttcgggtcgc aaatcacatt cggtctggtt ccggcgatga
      781 ttgccggaat aatgccgagc ccggcggcgt cgaaagcgcc gagagccgag ttgattaccg
      841 gagttaaggg cagcccggtc gagtatgtaa cgatgcccga tacctgccag ccgccgagaa
      901 ctttaccggc aaaatcattt cgattgttaa agaacggcac ttcataaaca tagttcatcg
      961 agaaaatatg gcggcggtcg agcgatgctc tgccccattc gctttcgata tcatatgaat
     1021 tctgcggcgc cgtcgaacgg tcggtctgat tattggtcag atttttcgac caggtgtatg
     1081 caaaatttac ctgcgaagcg ccgctgaagc gatgctgcgc ggaaaattgc atactgtggt
     1141 aattagagtc gtaacgcggc tgaaccatat tgattgactg gtagccgcga taggggcgaa
     1201 gctggtcgag aatgcgggtt tgcgcgccgg taaaaaatgc ggttcccgca acctgacacg
     1261 gagcggtcgg cgtcgtcgaa gcgccgaccg cgcagccggt cgcgccgagg ctgatggcgt
     1321 atcccggcgg cagagcattc aactcgtacg cgccgattaa gttcgtgcct ttcgagccgt
     1381 agtaacctat ggtaacaagc gttttgctgg ttagctgctg ctgaaggtcg agcgaccagt
     1441 gctgcatata cggcgttttc caatcagcct gcacgccgcg aatattcggg gctgccagcg
     1501 aagaagccac cgtgcagttg ccgcccggaa ccggatctgc cagattaact ccggtcacgg
     1561 tgcaggtttg ctggtagggc ggattcaaac cgatttgctg aagctgattt ccgttcagca
     1621 cctggtcgtg ataaatgccg tagccggtgc ggattgaagt ttcgcctttt ccgaacgggt
     1681 cccaggctaa accgacgcgc ggcgcgaaat cggttttggg cgcatcgata acaaacttgc
     1741 cccagggcga cggagtcggc gtgcagttaa aagcagccgg tccggtcgtg taattctgcg
     1801 tgttggcaat aagaccgtta cagaaattct tgccggctcc ggcgacgcgg ttgcccgcgc
     1861 ccgtaacgag cggcgctgcc gattggttga acatttcggg gatgaagttt gtcagacgac
     1921 cgtttttgtc ccacggcgag ccgaagaacg aataacgaac gcccatatat aaagtcagat
     1981 ttttgcggac gcgccattcg tcctgcgcaa atccttcaat cgttttctgt ctcaagtccg
     2041 ccgtgtaatc gaaactcgcc tgcgtaaagc tgacgtttgt tccgagcaga aagtttgcaa
     2101 actgctgctg cgaggtttgc gtgccgcacg gttgctgcgc aggaatgcag cctggagcag
     2161 gcaaagcggt agttgctccc ggcgtattga aaccgctgaa aataccttcg ttattaccgg
     2221 cgatggcatt ttcgtttttg cgatagagcg agtagccgcc gccgaatttc atcgtatggt
     2281 cgcccattat ccaggtaaag ctgccgctcc agttatgctt gtcggaaaag ttgtcgtatg
     2341 gaccgaatcc ctgcaaagcg ctgaatccgt tgccggaaat agtcggaatg cggtcgcggg
     2401 tattggcaaa cggaagcgga acgttaatcg tcgaattggt taaggcgagc aatccgatgt
     2461 tttcgctgag aatcgcgccg taactatagg tgtagcgagc ttcgataatc attcgcggat
     2521 tcaaaacata agtcgactgg acggtgtgag ctttgcccgg cgagtcggtc gccgtcgtcg
     2581 agactccggg aagcccggaa ccgctggaga acagcgaatt ggcttctatc gtcggaatcg
     2641 cgtcctgctg gaaccggtaa tacatcgacc agtttttcgt aaaattgtgg tcaatcttga
     2701 gtatttcctg acgaaagtcg gatttattga gagtcgggaa caataaactg ttcgcgccgg
     2761 aattcggcgc cggcagctta ttgtagatgg ctgtcagata ttgctgcgcg acagtattaa
     2821 tcggtcgcag cgtcgaaagg ggcgtgccgg ctggcagaat cgtcgtgcag gtccctgccg
     2881 tcgcctgcag gcaaatcggg acggtaaaaa cgccctgtct ttgagccagg ctcggcactg
     2941 tcgaagttag cgtcgggtaa cgtaaatcct ttctccattc ctgcgaaaag aagaaaaacg
     3001 ttcccttctg tctggcgaaa aacgagtcgt ccgggtcgcg
//
LOCUS       DASWNE010000300         5242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_361841, whole genome shotgun sequence.
ACCESSION   DASWNE010000300 DASWNE010000000
VERSION     DASWNE010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5242
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361841"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VGB68_06200"
     CDS             complement(<1..467)
                     /locus_tag="VGB68_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048854.1"
                     /translation="MKIAKIVKSNSHVDYVGRIIDSLDVAEPPATEDYGFAQFVSLPL
                     ENREEIVGVIYNSILINPDYANYGPRLSPKPELGGFSPDYLNEQGLLVGILLLGTSRE
                     DGKVLHGVPRRVVPAGQDVYKIEPNQIKRFHSDENECLQIHYYSQIVAHAGLFA"
     gene            complement(494..790)
                     /locus_tag="VGB68_06205"
     CDS             complement(494..790)
                     /locus_tag="VGB68_06205"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HEY0048855.1"
                     /translation="MLESIYEEALCREFYLRGIKYRRQTEIDVIYKGKIIKGQRVDIL
                     VENELIVELKSISELPEVASAQILSYLKATGLKRGLLINFGAMKLVDGIRRFSL"
     gene            complement(920..1459)
                     /locus_tag="VGB68_06210"
     CDS             complement(920..1459)
                     /locus_tag="VGB68_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048856.1"
                     /translation="MNQTENGKRKTENAARESKGEQFSAFHSPSSVPVICSACGTEAR
                     RENARFCLVCGKLLLEGYQPLDALRASYRMQGKTFSPAEPKQAEEIQNLFEKNENSVS
                     QTAWACVVYSFVPYLGILFVPFAFLVGSFGVLVSYRKPYLGGRKLSLVSVGLSFVVLV
                     VQIFLWWLLYFIPEIGKQL"
     gene            complement(1456..2667)
                     /locus_tag="VGB68_06215"
     CDS             complement(1456..2667)
                     /locus_tag="VGB68_06215"
                     /inference="COORDINATES: protein motif:HMM:NF020933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HEY0048857.1"
                     /translation="MLFRDLLTTELHKQSESFKRFALNQEDDLSEYLEKFSRFCRTPY
                     EEILAILETAKECGAIPSEEFDTARRFSVPFNQSWRNHEEARRWAFEILQNRTTFAAD
                     GSQLFAEREVSLPVAAIQIGWFENPHNDAVGYEKNVHFFVLSPEELLSPDEPFIRETR
                     VGQRRFAAEIEKIMEFLAKKRGWQARGERMPLAFYDNTLLLSISLPDKDFQLEFIDKL
                     VALVRLSRETEVPLVGYVDRSYARDLLSLLDAVDGTKAENKRTLDDSSILHAFTSEAL
                     PTLQNWGDRTIFCYSKRRGLSAFIDPETGKSIVGFTYLQTTSDSAPARLDVPSWVYEK
                     GLLNELVDTVRAECVIGLGYPYALETADQTAVITVRDREIFLRTLQDFAMREKLNFGV
                     SRKAASKGRRR"
     gene            complement(2742..4073)
                     /locus_tag="VGB68_06220"
     CDS             complement(2742..4073)
                     /locus_tag="VGB68_06220"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0048858.1"
                     /translation="MKFIRYSKFKGFDVFGVSLGDLMQSLSDSLLDSGYSEDYWWTRR
                     RNETDDSLDALRQAILKALLEQEILTPPDVKQMLAENEGKFKGSLLEELINQLIERLV
                     DEGYLNLKEIEQPQARQQQQNGEGQGEIGQPVPRDVRFEVTEKGLDFLGYKTLQKLLG
                     SMGKSSIGRHDTHYLDTGIEASQAAKAYEFGDTINLDVNATLLSAIAREGLKVPLNLE
                     YKDLYVHQQDYHSSCATVVMLDCSHSMILYGEDRFTPAKKVALALAHLIRTQYPGDSL
                     KIVLFHDGAEELPLAKLATTQVGPYHTNTAEGLKMARRILAAQRKEMKQIVMVTDGKP
                     TAAFVEPSNAAFFSHRRYSQSVKGEKRLLYKNSMGNDPFVMAETFKEVQACRKAGIMI
                     NTFMLASDWYLVEFVKQMSSMTRGKAYFANPNNLTQFVLMDFLKRRTSRVK"
     gene            4253..4642
                     /locus_tag="VGB68_06225"
     CDS             4253..4642
                     /locus_tag="VGB68_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048859.1"
                     /translation="MKKFAAGLLVLFVLMIGLPLSAEAHNGCPRHRRASRSSVSRNYY
                     RQAPRYRSARTYRSYRTYRTAGYSYQRPSFYRRHRNLINLGIATGGGAIIGGIAGGRR
                     GAGIGALTGLGAGALYTYVLNKKRRRY"
     gene            4920..5237
                     /locus_tag="VGB68_06230"
     CDS             4920..5237
                     /locus_tag="VGB68_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048860.1"
                     /translation="MKKFLTAFVMMAIMAIMIPLLSTETNAQTRRSYRNRTSTTRVYN
                     RPSFYRRHRNVINMGIGTAAGAILGGIIGGKRGAGIGALTGLGGSALYTYKIKPKQRR
                     YYR"
BASE COUNT         1313 a         1345 c         1262 g         1322 t
ORIGIN      
        1 gcgaaaagtc cggcgtgagc gacgatttgc gagtaataat gaatctgcaa acattcgttt
       61 tcgtccgagt gaaaacgctt tatctgattc ggctcgattt tataaacgtc ctgtccggcg
      121 ggaacgacgc ggcgcggaac gccgtgcaga acttttccgt cttcgcggct cgtgccgagc
      181 agcaggatgc cgaccagcaa gccctgctcg ttcagataat cgggactgaa gccgccgagt
      241 tccggcttcg gcgaaagccg cggtccgtaa ttggcgtaat ccggattgat gagaatcgaa
      301 ttgtaaatca cgccgacgat ttcttccctg ttttccagcg gcagcgagac aaactgcgca
      361 aaaccgtaat cttcagtcgc cggcggctcg gcaacgtcca aagaatcgat aatgcgcccg
      421 acataatcaa cgtgggaatt cgattttaca atctttgcaa ttttcataga tttagccaca
      481 gaggacaccg agcttacaga gaaaatcttc taattccgtc aactaatttc atcgctccaa
      541 agtttattaa caaacctcgt tttaacccgg ttgctttcag ataagacaaa atttgagcag
      601 aggcgacttc gggtaattcg gaaattgatt ttaattcaac gataagctca ttttctacga
      661 gaatatcgac tcgttgtcct tttataattt tacccttata gataacatca atttcagttt
      721 gtcgtcggta tttaattccg cgtaaataaa attcgcggca aagagcttct tcataaatag
      781 attccagcaa accacaacct taatttctgt ggacttcaat tgccgctccg attatttttt
      841 gcgtgagaaa atcgttcata aatatactca actaatatta atgaaacttt tctctgtgct
      901 ctctgtgtcc tttggtggct taaagctgct ttccgatttc gggaataaaa taaagaagcc
      961 accaaaggaa aatctgcacg accagaacca caaaactcag accgacgctg acgagcgaca
     1021 attttctgcc gcccagataa ggcttgcgat aggaaaccag aacgccgaag ctcccgacca
     1081 gaaaggcgaa gggcacgaac aaaattccca ggtaaggcac aaacgaataa acgacgcacg
     1141 cccacgccgt ttgggaaact gaattttcgt ttttctcaaa caggttttgt atttcctcgg
     1201 cttgtttcgg ctcggcgggc gaaaaagttt ttccctgcat ccggtaagat gcgcgaagcg
     1261 cgtccagcgg ttgataacct tcgagcagaa gtttgccgca gaccagacag aaccttgcgt
     1321 tttcgcgccg cgcttcggtt ccgcaggcgg aacaaattac cggcacggaa gacggagaat
     1381 gaaaagcgga aaattgctcg cctttcgatt cacgcgccgc attttccgtt ttccgttttc
     1441 cgttttccgt ctgattcatc ttcttctgcc tttgctcgcc gctttgcgcg agactccgaa
     1501 gtttagtttt tcacgcatcg caaagtcttg taaagtcctg agaaaaattt ctctgtccct
     1561 gaccgtgata accgccgtct ggtcggcggt ctccagcgcg tacggataac ccagcccgat
     1621 aacgcattct gctctgacag tgtcgacaag ctcatttaaa agaccttttt cgtaaaccca
     1681 ggacggaacg tccagacgcg ccggagcgct gtcggaggtc gtttgcagat aggtaaagcc
     1741 gacgatggat tttccggttt ccgggtcgat aaaagcgctt aagccgcgcc gcttcgagta
     1801 acaaaaaatc gtgcggtcgc cccaattctg caaagtcggc aaagcttccg aggtaaaggc
     1861 gtgaagaatt gaagaatcgt cgagcgtgcg tttattttcc gctttcgttc cgtcgaccgc
     1921 atcgagcagc gaaagcaggt cgcgggcgta gcttctatcg acgtaaccga ccagaggaac
     1981 ttccgtctcg cgcgaaagac gaacgagcgc gaccagctta tcgataaact caagctggaa
     2041 atccttatcc ggcaatgaaa tcgagagcag caacgtattg tcgtaaaaag ccagaggcat
     2101 tctttcgccc cgcgcctgcc aaccgcgctt ttttgccaga aattccataa ttttttcgat
     2161 ttcggcggcg aaacggcgct gaccgacacg ggtctcgcgg ataaacggct catccggcga
     2221 caaaagctct tccggcgaaa gaacgaagaa atgcacgttc ttttcgtagc cgacggcgtc
     2281 gttatgcgga ttttcaaacc agccgatttg aatcgccgcc accggcagcg aaacttcccg
     2341 ctcggcgaaa agctggcttc cgtccgccgc aaacgtcgtc cggttctgca aaatctcgaa
     2401 cgcccagcgg cgcgcttctt cgtgattgcg ccaggattga ttaaagggaa ccgaaaaccg
     2461 gcgcgccgta tcgaattctt ccgacggaat cgcgccgcat tctttcgccg tttccagaat
     2521 cgcgagaatt tcttcgtaag gcgtgcggca aaatcgcgag aatttctcca gatactcact
     2581 caaatcatct tcctgattca gcgcaaaacg cttgaagctc tcgctttgtt tgtgcagttc
     2641 ggttgtcaga aggtcgcgaa aaagcattgt aattttcggt aataaaaagt tttcgttttt
     2701 tatatcaagc tgatcggaaa ccgggcagga gccggaaata attatttcac gcggctcgtc
     2761 cgccgtttca gaaaatccat caaaacaaac tgcgtcaggt tgttcgggtt ggcgaaatag
     2821 gctttgccgc gcgtcatcga cgacatctgc ttgacgaatt cgaccagata ccaatcgctc
     2881 gccagcataa aggtatttat cataattccg gctttgcggc aggcttgcac ttccttgaaa
     2941 gtttccgcca taacgaacgg gtcgttgccc attgagtttt tatacaaaag acgtttttcg
     3001 cctttcacgg attgcgaata acggcgatgc gagaaaaacg cggcgtttga aggctcgaca
     3061 aaagcggcgg tcggcttgcc gtccgtgacc atcacgattt gtttcatttc cttgcgctgc
     3121 gcggctagga tgcggcgagc cattttcaaa ccttcagccg tgttcgtatg atagggtccg
     3181 acctgcgtgg tcgccagttt cgccagcggc aattcttcgg cgccgtcgtg gaaaaggacg
     3241 attttcaggc tgtcgcccgg atactgcgtc cggatgagat gcgccagcgc gagcgcgact
     3301 tttttggcgg gcgtaaaacg gtcttcgccg taaagaatca tcgaatgcga gcagtcgagc
     3361 atcacgacgg tcgcgcacga cgaatgataa tcctgctggt gaacgtataa atccttgtat
     3421 tcgagattga gcggaacttt taagccctcg cgcgcgattg ccgaaagcag cgtcgcgttg
     3481 acgtccagat ttatcgtgtc gccgaactcg taggcttttg ccgcctggct ggcttcgatt
     3541 cccgtatcca gataatgcgt atcgtgtctg ccgatgctgg atttgcccat cgagccgagc
     3601 agtttttgca gtgttttata gccgagaaaa tccaagcctt tttcggtcac ttcaaaacgc
     3661 acgtcgcgcg gcacgggctg cccgatttcg ccctgacctt cgccgttttg ctgctgttgc
     3721 cgcgcctgcg gctgctcgat ttctttcaga ttcagataac cttcgtcaac cagtctttcg
     3781 ataagctgat ttatcaattc ttcgagcaac gagcctttga atttgccttc gttttcggcg
     3841 agcatctgct tgacgtcggg cggcgttaaa atttcctgct ccagaagcgc ttttaaaatc
     3901 gcctgccgca gagcgtccag cgaatcgtcc gtttcattgc gccggcgcgt ccaccagtaa
     3961 tcctcgctgt aacccgaatc cagaagcgaa tccgacagcg actgcatcaa atcgccgaga
     4021 ctgacgccga acacgtcgaa acctttaaat tttgaatagc gtataaactt catcgcgtta
     4081 aatttccctg caaactctaa aaaatttagt tgccatccgc cgaccaaatg tttatatttg
     4141 gggttatagc tgttttgaaa aaggctcttg ccaaaaaaaa ttctgctctc ccaaaaactt
     4201 aacacattca ctctcccgaa tgaaataaat tttagattaa ggagacaagt atatgaaaaa
     4261 gtttgcagca ggattgctgg tcctatttgt tttgatgatt ggtttgcctt tgtcggcaga
     4321 ggcgcataac ggatgcccgc gtcaccgccg ggcaagtcgc tcgtccgtca gccgtaatta
     4381 ttaccggcag gctccgcggt atcgcagcgc gcgcacatac cgcagttatc gcacatatcg
     4441 taccgccggt tattcctatc aaagaccgag cttttaccgt cggcatcgca acctgattaa
     4501 tctgggcatc gcgaccggcg gcggcgctat aatcggcggc atcgccggcg gcagacgcgg
     4561 cgcgggcatc ggcgctttga ccggattggg cgcgggcgcg ctttacacgt atgtgctgaa
     4621 caagaaacgg cgtcgttact aattcagcct tttcaaaatt tatctattta gagaaagccg
     4681 taatttttaa attcttacgg cttttttcct tgcctgaaaa ttctttaaat taaaccgatt
     4741 aaatacaccc caaaacgtcg aacgtaaacg aaatagtgtc gggaagcagc aggttaaaaa
     4801 ctgccgaaac cggctaaatc attgaataat ctaagttttt tctttgtggc acggcgattg
     4861 aatatagata ggcagccgaa ggtaaaaacc ttcaaatttt ataaggggaa gataaagcaa
     4921 tgaaaaaatt cttaacagca ttcgtgatga tggcaataat ggcgattatg attccgttgc
     4981 tgtcgactga aacaaacgcc cagacgcgaa gaagctacag aaatcgcact tcgacgacgc
     5041 gcgtttacaa ccgtccgagt ttttatcgtc gtcaccgaaa cgtgattaat atgggcatcg
     5101 gaacggcagc cggcgcgatt ctcggcggca ttatcggcgg caagcgcggc gcgggtatcg
     5161 gcgctttgac aggcttgggc ggctcggctc tttatactta caaaatcaag ccgaaacaaa
     5221 gacgctacta tcgttaattc ac
//
LOCUS       DASWNE010000301         1866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_150591, whole genome shotgun sequence.
ACCESSION   DASWNE010000301 DASWNE010000000
VERSION     DASWNE010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150591"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VGB68_06235"
     CDS             complement(<1..511)
                     /locus_tag="VGB68_06235"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY0048861.1"
                     /translation="MKNQHLFIRKIIIVILLMVGLSAAIRGQQNMTAKLQPSDEESAK
                     KVDEFLAQWDKNDMPGCSVGVVKDGRLVYKRAFGMANLDYDVPNTPSTVFNLASASKP
                     FTAFSIALLAQQGKLSLDDDIRKYVPEFPKYDETVTIRHLLYHTSGIREYQALVFFSG
                     LGADNAYSDK"
     gene            complement(1049..1255)
                     /locus_tag="VGB68_06240"
     CDS             complement(1049..1255)
                     /locus_tag="VGB68_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048862.1"
                     /translation="MNESLALAAPEWKKETKARDIVMAIRRAATSDNQWVRFWGSFIV
                     ELGRRGKVPFDLRNDEEVKYSFGF"
     gene            1366..>1866
                     /locus_tag="VGB68_06245"
     CDS             1366..>1866
                     /locus_tag="VGB68_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048863.1"
                     /translation="MRLRKVFTDLLKQSLSLILLTAILSAAFSVAAQTRRATRPTGKR
                     PASPQTAKIPVVKDAEVCTGGWSGVINYERTLDESGFKDDGGGSFRKWSHNVSYEGRM
                     IVDGSNPQAVKTIADVEFSDVRRQQEKVVMRGQCGAWRPEHDFIVDNKLEDIEKGTGE
                     RLAGNFD"
BASE COUNT          455 a          477 c          475 g          459 t
ORIGIN      
        1 ttttgtcgga gtaggcgtta tcggcgccga gcccgctgaa aaagaccagc gcctgatatt
       61 cacggattcc gctggtgtga taaagcaaat gccggattgt gacggtctcg tcgtatttcg
      121 gaaattcggg aacgtacttg cgtatgtcgt cgtcgagcga caattttccc tgctgcgcca
      181 aaagcgcgat gctgaacgcc gtaaatggtt tcgaagccga agcgagatta aacacggtcg
      241 aaggcgtgtt cggaacgtcg taatcgagat tcgccattcc gaaagcgcgt ttgtaaacaa
      301 gcctgccgtc tttaaccacg ccgaccgaac atcccggcat atcgtttttg tcccattgag
      361 caagaaactc atcgactttc tttgccgatt cttcatcgga aggctgcagc ttagcggtca
      421 tattctgctg tccccgaatg gcggcgctca aaccgaccat caataaaatg acgatgataa
      481 tttttcgtat aaataaatgc tggtttttca tttctttgtt tctcgtgtaa caactccaaa
      541 gccgttacga aggtaaataa cgtttctccg tgcccgcagg tttcgtcgga gacgcctttc
      601 agagccgctg acgcgatttc taatctcggc gtgtaggatg attatcgaac tcggacgagc
      661 aacggctggt gcctgttcgg gcggtcttcg ccgcacttgt ccgaagcgcg tgtggttgct
      721 ccgccgctcg cggctcatct cttcgctcag ttcgggcaag tcgtctgacg cggaagcagg
      781 cgcactctgt ttgccgtcgc cctgcaaaga agattttgaa tcgtcacccg cgaatataaa
      841 cgtgcgcggc ggcgaattat taacaacaaa gataaataaa tttctcgcat ccgttgaaag
      901 aattatatct tctccgggtg tttagtcccg taaagagtcg ttttcgggat ttttcataaa
      961 cgggcgtttt cgaattgcaa cagctccgcc tcattcgata aatgattata aagaccggta
     1021 ataaataacc aggttaacga tgattctctc aaaaaccgaa agagtatttt acttcctcat
     1081 cgtttcgcaa atcgaacggc actttgccgc gccttcccag ttcaacgatg aaacttcccc
     1141 aaaaacgcac ccattgattg tcgctcgtcg ccgctcgtcg aatcgccata acgatatccc
     1201 gcgccttcgt ctcctttttc cattcgggcg cagcgagcgc gaggctttcg ttcaaacgga
     1261 aaaaaatatg actctttttt gctgaacctt ttccgccgcc ttttgcgctt tagatattga
     1321 agccaaaaac gtcggcgata tttgccgccc ggagacttga aaagaatgcg attacgaaaa
     1381 gtttttaccg atttgctcaa acaatctttg agcttaattc tattgaccgc tattttgagc
     1441 gctgcatttt ccgtcgcggc gcaaacccgc cgcgcgacgc gcccgaccgg gaaaagaccc
     1501 gcttcgccgc aaaccgcgaa gattccggtc gtaaaggacg cggaagtgtg tacgggcggc
     1561 tggagcggcg tgataaatta cgagcggacg ctcgacgaat ccggtttcaa ggatgacggc
     1621 ggcggcagct ttcggaaatg gtcgcataat gtttcctacg aaggcagaat gattgtcgac
     1681 ggcagcaacc cgcaagcggt gaagacgatc gccgatgtcg aattcagcga cgtgcggcgg
     1741 cagcaggaaa aggtcgtcat gagaggacag tgcggagcct ggagaccgga acacgatttt
     1801 atcgtcgaca acaaactcga agatatcgaa aagggcacgg gcgagcgact ggctggaaat
     1861 ttcgac
//
LOCUS       DASWNE010000302         5077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_539867, whole genome shotgun sequence.
ACCESSION   DASWNE010000302 DASWNE010000000
VERSION     DASWNE010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5077
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539867"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /gene="aat"
                     /locus_tag="VGB68_06250"
     CDS             <1..474
                     /gene="aat"
                     /locus_tag="VGB68_06250"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEY0048864.1"
                     /translation="EELHVPRSLRKAREKSDFTFTIDKNFVEIIINCARVRRKGQYGT
                     WITQEVFDSYSELHRLGHVHSVEVWDAAGNLVGGLYGVDAGGVFCGESMFHRAPNASK
                     LALLYLVEHLAGRGSTWLDVQVMTPHMKILGAKEIDREDFLDKLQTTLTENLKLF"
     gene            549..1613
                     /locus_tag="VGB68_06255"
     CDS             549..1613
                     /locus_tag="VGB68_06255"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEY0048865.1"
                     /translation="MAFNNLMKKNFLIYGANGYTGELVLRYARERGLKPLIAGRNAEK
                     IEAAARKFGVDFRVFSLEDTTRLDAALSEVDFVIHCAGPFSLTSKQMVEACLRTGKHY
                     TDITGEIAVFEAMARLDARAKEAKIMIMPGVGFDVVPSDCLARHLKNRLPSATHLTLA
                     FYGMGRISHGTRATMTMNAGGGGAIRQNGEITRVPAAWKSREIDFGEVKKTGTTIPWG
                     DVSTAFYSTGIPNIEVYTVVPETARKMMKLSRYLGWLLAAKPVQNYLQKQIPPGGPGD
                     EERARGKTLLWGAASDAQGNRVESRLTAPEGYTTTVLTALQIAEKILDGNFCDGFQTP
                     AKCYGADLILEIEGVKRNDE"
     gene            complement(1689..2126)
                     /locus_tag="VGB68_06260"
     CDS             complement(1689..2126)
                     /locus_tag="VGB68_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048866.1"
                     /translation="MRKLKFFLTAVLVCCFAGNAFGAVNSERQDAAFKAFWAKFKAAV
                     AKKDKNAAANLSRFPISMPAFQKSVRTKADFLRRYSKIFDGEADAAKCFRNATPRKAS
                     AKSYEVNCGFKNDPNGDGGEPIVYSFELTKSGWKFVGLDNINE"
     gene            2203..3609
                     /locus_tag="VGB68_06265"
     CDS             2203..3609
                     /locus_tag="VGB68_06265"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HEY0048867.1"
                     /translation="MNRRNFLWRISAAGTALAFGGLTRRAEALLETKNFSSLRAAGFG
                     ELVPAAAKNTGETFLALPKGFEYNVIGKIGSVMADSNKTPRAHDGMAAFKVGGELRIV
                     RNHEVSNGRVPKDGAAIGANPYDETVAGGTTTLVINPKTRELTRDFVSLSGTLINCAG
                     GRTPWGSWISCEETTLGQTVRISPKGVKTGGYPKPHGYCFEVFASANSSVTPVPLKAM
                     GRFVHEAVAVDKKSGIVYLTEDYNPAGFYRFLPKRHKRLAAGGTLQMLKIKDKDNFDT
                     RSGQKTGMTFVADWVTIDNPDPAEADVDEMAVYKQGKAKGGAGFARLEGCYADPKGGR
                     IYFTSTSGGDNKGGQVWLYEATGRDEGRLTLVFESPSRDVLDMPDNICLKPKSGLLFI
                     CEDSDYVGEGGTPQNFIRILTPDGKMADFAKNITPNFIASEFAGSTFSKDGKTLFFNL
                     QSVGATFAVWGDWDKFRS"
     gene            complement(3735..4010)
                     /locus_tag="VGB68_06270"
     CDS             complement(3735..4010)
                     /locus_tag="VGB68_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048868.1"
                     /translation="MAETENSTAAAEAIEIVEPTDAEKAASAAVNEANAAANEGAESA
                     EDISYHAVEKEGSVTAIWEMQGRRHIRTIDPRSGEGKQILALFETAG"
     gene            complement(4194..4706)
                     /locus_tag="VGB68_06275"
     CDS             complement(4194..4706)
                     /locus_tag="VGB68_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY0048869.1"
                     /translation="MLTEETKKLSISLTSGVFTNEEFIQAYSDGRRIDKEYVINLFNE
                     AINNKDAEAVEEALTVGIVFDFFSNEFSEIFCKLLQADWHYKHEDIAGLLQDLKDPST
                     VDCLYNAAQLHFEYLNYDDSYQFARKCIKALSKMGNKEAIEKLQLLAGSNIPEIKQYA
                     VKELKYKGLL"
     gene            complement(4778..>5077)
                     /locus_tag="VGB68_06280"
     CDS             complement(4778..>5077)
                     /locus_tag="VGB68_06280"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEY0048870.1"
                     /translation="IGDFIPSGANVYVVVMTLGYKSDAAVIRRLFDKDFKYFGVLGSG
                     AKMKILLKNLRREGFSAEKLNQIHTPVGLPINSRTPEEIAVSIAAEIIAIKNIKD"
BASE COUNT         1251 a         1254 c         1307 g         1265 t
ORIGIN      
        1 gaagagcttc acgttccgcg cagcttgcgc aaagcgcgcg agaaatccga ctttaccttc
       61 accattgata aaaattttgt cgagattatc ataaactgcg cgcgcgtccg ccggaaaggt
      121 caatacggaa cgtggattac gcaggaagtt ttcgacagct acagcgagct tcaccgtctc
      181 ggacacgtcc acagcgtcga ggtttgggac gccgccggca atctggtcgg cggtctttac
      241 ggcgtcgatg cgggcggcgt tttctgcggc gaaagtatgt ttcaccgcgc gccgaacgct
      301 tcaaagctcg cgctgcttta tctggtcgaa catctcgccg ggcgcggctc gacgtggctc
      361 gacgtgcagg taatgacgcc gcatatgaaa attctcggcg caaaagagat agaccgcgaa
      421 gattttctgg acaagctgca aacgacttta acggaaaacc tgaaactttt ctgacgattg
      481 atttaaacag gatgaacgga aatcatcggg attagcccgc attgtcatta gctcgtatta
      541 tccttttgat ggcgtttaat aatttaatga aaaaaaactt tctcatctac ggcgcaaacg
      601 gctacacggg cgagcttgtt ttgcgttacg cgcgcgagcg cggattaaag ccgctgattg
      661 ccggcagaaa cgcggaaaaa atcgaagcgg cggcgcgaaa attcggcgtc gattttcgcg
      721 ttttttcgct ggaagacaca acgcggctcg acgcggctct gagcgaagtc gattttgtga
      781 ttcactgcgc cggaccgttt tcgctgacct cgaaacagat ggtcgaagcc tgcctccgaa
      841 ccggaaaaca ttacaccgat attacgggcg aaatcgccgt ttttgaagcg atggcgcggc
      901 tcgacgcgcg ggcgaaagaa gcgaaaatta tgattatgcc gggcgtcggt ttcgacgtgg
      961 tgccgtcgga ttgtctggcg cggcatctga aaaatcgcct gccgtcggca acgcacctga
     1021 cgctggcgtt ttacgggatg ggcagaatct cgcacggcac gcgggcgacg atgacgatga
     1081 atgccggcgg cggcggcgcg attcggcaaa acggcgaaat cacgcgcgtt ccggcggcgt
     1141 ggaaatcgcg cgaaatcgat ttcggcgaag tcaaaaaaac gggcacgacg attccgtggg
     1201 gcgatgtttc gacggcgttt tattcgaccg gaattccgaa tatcgaagtt tacacggtcg
     1261 tgccggaaac ggctcggaaa atgatgaagc tcagccggta tctcggctgg cttttagccg
     1321 cgaaacccgt gcagaactat ctgcaaaaac aaattccgcc gggcggtccc ggcgatgaag
     1381 aacgcgcgcg gggcaaaact ctgctctggg gcgcggcgag cgacgcgcag ggaaatcgcg
     1441 tcgaatcgcg cctgaccgcg cccgaaggct acacgacaac cgttttaacc gcgctgcaaa
     1501 tcgcggaaaa aattctggac ggaaatttct gcgacggttt tcaaactccg gcgaaatgct
     1561 acggcgctga tttgattctg gaaatcgaag gcgtgaagag aaacgatgaa tgatgaacgc
     1621 ggaacgatga attgcaattt tcgcgctttt gtttttaact tggcattcat cgttccgcgt
     1681 tcatcgtttt attcgttgat gttgtccaag ccgacgaatt tccagcccga tttcgtcagc
     1741 tcgaacgaat agacaatcgg ctcgccgccg tcgccgttcg ggtcgttttt gaaaccgcag
     1801 ttgacttcat agctttttgc cgaagctttt cgcggcgtcg cgtttcggaa gcatttggcg
     1861 gcgtcggctt cgccgtcgaa aatcttgctg taacgacgca gaaaatcggc tttcgtgcgc
     1921 accgattttt gaaacgccgg catcgaaatc ggaaaccgcg aaagatttgc cgccgcattt
     1981 ttgtcttttt tcgcgacggc tgctttgaat tttgcccaga aagctttgaa tgccgcgtcc
     2041 tgacgctcgg aattgaccgc gccgaaagcg tttccggcaa aacagcaaac taaaaccgcg
     2101 gttaaaaaga atttaagttt tctcatattt accttccgaa tcatatagat ttataatgct
     2161 ttcagacgca ttctaaccgg atattttgct tttgcaacat ttatgaaccg acgaaatttt
     2221 ttatggcgga taagcgccgc cggaactgcg ctggcgttcg gcggcttgac gcgccgcgcc
     2281 gaagctttac tcgaaacgaa aaacttttcc agcctgcgcg ccgcgggttt cggcgagctt
     2341 gtcccggcgg cggcgaaaaa taccggcgaa acttttctgg ctttgccgaa gggatttgaa
     2401 tacaacgtca tcggcaaaat cggaagcgtg atggcggaca gcaataaaac gccgcgcgcg
     2461 cacgacggaa tggcggcttt taaagtcggc ggcgagctgc gcattgttcg caatcacgaa
     2521 gtgtcgaatg ggcgcgtgcc gaaagacggc gcggcaatcg gcgcaaatcc ttatgacgaa
     2581 acggtcgcgg gcggaacgac gacgctcgta atcaatccga aaacgcgcga gctgacgcgc
     2641 gattttgtta gcctgagcgg aaccttaatc aactgcgcgg gcggcagaac gccctgggga
     2701 agctggattt cgtgtgagga gacgacgctc ggacagacgg tcagaattag cccgaaaggc
     2761 gtcaaaacgg gcggttatcc gaagccgcac ggttattgct tcgaggtttt cgcctcggca
     2821 aattccagcg tcacgcccgt gcctttaaaa gcgatgggac gtttcgttca cgaagccgtc
     2881 gccgtcgata aaaaatccgg catcgtctat ctgaccgaag attataatcc ggctggattt
     2941 tatcgttttc tgcccaaacg ccataaacgc cttgccgcgg gcggaacgct gcaaatgctg
     3001 aaaatcaagg acaaagataa tttcgacacg cgcagcgggc agaaaacggg aatgactttt
     3061 gtcgccgact gggtgacgat tgacaatccc gacccggctg aagcggacgt tgacgaaatg
     3121 gcggtttata aacagggaaa agcaaaaggc ggcgccggtt tcgcgcggct cgaaggctgt
     3181 tacgccgacc cgaaaggcgg aagaatttat tttacttcga ccagcggcgg cgacaacaaa
     3241 ggcgggcagg tctggctcta tgaagcgacg ggcagagatg aaggacggct gactctggtt
     3301 tttgaatcgc cgagccgcga cgttctggat atgcccgata atatctgtct gaaaccgaaa
     3361 agcggattgc tttttatatg cgaagacagc gattacgtcg gcgaaggcgg aacgccgcaa
     3421 aattttatcc gaatcttgac gccggacgga aagatggcgg attttgccaa aaacatcacg
     3481 ccgaatttta tcgcgagcga atttgccggc tcgacgtttt ccaaggacgg caaaacttta
     3541 tttttcaatc tgcaatcggt cggcgcaact ttcgccgtct ggggcgattg ggacaaattt
     3601 cgaagctgac gccggaattt aaaataaaaa aagggaaatc gaaagtttaa aattttaaac
     3661 tttcgatttc cctttttaga aagagactgc ggcgcgtcgt cgccggcgtc cgtttatctt
     3721 tttgtcgcca atctttatcc ggcggtttca aacaaagcca aaatctgttt gccttcgccc
     3781 gagcgcgggt caatcgtgcg gatatgcctg cgaccctgca tttcccaaat cgccgtcacg
     3841 ctgccttctt tttccaccgc gtgatacgaa atatcctcgg cgctttccgc accttcgttt
     3901 gctgccgcgt tcgcttcatt gactgccgcg ctcgctgctt tttcggcgtc ggtcggttcg
     3961 acgatttcaa tcgcttcggc tgccgccgtg ctgttttcag tttctgccat tcaaatcctc
     4021 tcttaaaata ttattcttaa attattttga ttaaacaaat tttgtcacag cctgcctcgt
     4081 cacgcaagct tcaggctcaa aaattgacgg tttgacagcc agcagacagg agaaaaaatt
     4141 ccgttggaac ataactatcc cttacccgag gtattgcccg caaaatcggc attttacaat
     4201 aatcctttat atttgagttc tttgacggca tattgtttta tttcgggaat attactacca
     4261 gccaaaagct gaagtttttc tattgcctct ttattaccca ttttagacag tgctttgatg
     4321 cattttcggg caaattggta tgagtcatca taattaagat actcaaaatg cagctgagcc
     4381 gcattatata aacaatctac agttgaaggg tctttcaaat cttgtagtaa tccggctata
     4441 tcttcatgtt tatagtgcca gtcagcttgc agaagtttgc aaaaaatctc ggaaaactcg
     4501 tttgagaaga aatcaaagac aatgcctacc gttaatgctt cttcaacggc ttcggcatct
     4561 ttgttattaa tcgcttcatt aaataaatta ataacgtatt ctttatctat tctcctgccg
     4621 tctgaatatg cctgaataaa ttcttcattt gtaaagacac cagatgtcag tgaaatcgac
     4681 agttttttag tttcttcggt cagcattcta tctattttaa atagagcgcg attctaccat
     4741 aatttccaag cttattacta caaaaaatca aacgcggcta atcttttata ttttttattg
     4801 caataatttc agccgcgatg ctgacggcga tttcttcggg cgttcggctg ttgatcggca
     4861 agccgacggg cgtgtgaatt tggtttaatt tttccgccga aaatccttcg cggcggagat
     4921 ttttgagcag gattttcatc tttgcgccgc ttcccaaaac gccgaaatat ttaaaatctt
     4981 tgtcgaaaag ccggcgaatc accgccgcgt ccgatttgta tccgagcgtc atcacgacca
     5041 cgtaaacgtt tgcgcccgac ggaataaaat cgccgat
//
LOCUS       DASWNE010000303         4892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_150627, whole genome shotgun sequence.
ACCESSION   DASWNE010000303 DASWNE010000000
VERSION     DASWNE010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150627"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..464
                     /locus_tag="VGB68_06285"
     CDS             168..464
                     /locus_tag="VGB68_06285"
                     /inference="COORDINATES: protein motif:HMM:NF003817.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEY0048871.1"
                     /translation="MALFSSDKFKCARCGRKDEQMAFAPFPNELGARVYNEICQTCWK
                     EWLQKQNQLINHFGLDVSSPDSHDFLFDNLKIFLFNEGVELTQIDTTQEGKVNW"
     gene            699..2969
                     /locus_tag="VGB68_06290"
     CDS             699..2969
                     /locus_tag="VGB68_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0048872.1"
                     /translation="MKHIRVLFALAALFIFSASASFAQTNGKISGKVTYGGDNTILHD
                     AAVQIVQMKRTISTDAAGNYEFTDIPPGRYTLLVHIEGFADATKIVDLKAGASQTADF
                     ELQISSVRADVTVTASGTEQSVFDSFQSVNALSSTQITQRASTSLGEVLESETGVAKR
                     SFGVGSSRPVIRGFDGDRVLVLQDGVRGGSLGSQSGDHGESVDPLSAERIEVVKGPGT
                     LLYGSNALGGVVNVIGNDENEAHAGFRGFFTALAGTADRQGGLAGGVEYGFKNWLFRG
                     NLSAQRSDNYATPLGIVPNSASRSNTGSFGVGYYGDKAFLSGTFNVDIRRYGIPFAAL
                     FEGGEKAAPNSLGGEIPFVDEDIDIRMRRRNFRVNGGFRNLNNSFLSSINYNLDYTNY
                     RHKEIEIADGIEEVGTIFDNKTFSYRSVFEQTKYNRLTGRFGFEGFDRDYEINGAEQL
                     IQGKVKHNSIAFFGLEELDFERVKFQFGGRVERNGYRPENPDLLERTFTGFSGGAGVN
                     FRLWKGGAFIANYTHSFRAPSLEELYNNGPHVGTISFEIGNENLRPERSNGLDFSLRH
                     LSDRFRIMGDVYYYRINNFVFQALQDDDGDGQVDIMEGLPVRRYEQQDSRYLGAEISA
                     DVTINKYLVFLASGDLVKAELTDGTPLPRIPPMRGRLGLDIRYKGLSVRPEGVFVGER
                     GQDDIFPLETPTDGYSLFNVAGSYTIGRQHFAHIFSFNAYNLTNKLYRNHLSFIKDLA
                     PEIGRGIRFSYTVRFF"
     gene            complement(3086..4312)
                     /locus_tag="VGB68_06295"
     CDS             complement(3086..4312)
                     /locus_tag="VGB68_06295"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0048873.1"
                     /translation="MKRNFYFLAFTLVMLLLALTVNNFAAPVVRQGAGANAAGLQAIV
                     DLFRADLGGSNNGVGGSFTSGRREVNWDGVPDNFAEPNNFPPNFFNVNSPRGVIFNSI
                     EDATGAALNQFAVSSNTASGVPVRFGNLNAAYTNIFQTFSAQRLFIARNTHILEVSFF
                     VPGTKIPATVSGFGVVFADVDSATGGNRSLIRVYGTDGSQLSAASAPVHDGGLSFVGI
                     SFNNGERIARVVIESGNAALSATNNDELDGVDVAAMDDFIYGEPRAAQHHESDFDGDG
                     TADLAVFRPGAGDWFVLNSGSNTFTGLHFGASGDLPVNGDVDGDSRNDFIVFRPSIGT
                     WFVLRSSDGQFAATQFGTSGDKPVMADYDRDGKSDLAVWRPSSGTYFILRSSNGQLQA
                     NQFGANGDIPVAASPQ"
     gene            complement(4349..4591)
                     /locus_tag="VGB68_06300"
     CDS             complement(4349..4591)
                     /locus_tag="VGB68_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048874.1"
                     /translation="MKPGRVRVIYNRRNRYLKWCACCCSEAGILTLEDAAKAAGIEPE
                     TICSFIGQGKIRLYQTSSDESAICLNSLLNIVNHLF"
BASE COUNT         1136 a         1299 c         1337 g         1120 t
ORIGIN      
        1 ggaggcgatt aaggaagccc ggaaaaccga gtatgtgaac tgaagagacg cccgaaggcg
       61 cagcgagcaa cgatgaattc tttgttccgc attcagcttt aggcgttcac gggttttcct
      121 gatttgtgtt aatctgtgat taaaaattgt atcagggagt tttcagaatg gctttatttt
      181 cgagcgataa attcaaatgc gcgcgctgcg ggcgcaagga cgagcagatg gcgtttgcgc
      241 cgtttccgaa cgagctcggc gcgcgcgttt ataacgaaat ctgtcaaacc tgctggaagg
      301 aatggctgca aaagcagaac cagcttatca atcatttcgg gctggacgtc tccagccccg
      361 actcgcacga ttttctgttc gacaatctga agatttttct ctttaacgag ggcgtcgagc
      421 tcacgcagat cgacacgacg caggaaggca aggtgaactg gtagggacga aggcaaaaag
      481 caaaaaagca aaagtaaaaa ggtaaaaggc aaaagcggtt tggcggattg ttcgcggcgg
      541 gcaatccgct tttctttgtt tttaccgaga ctgtttattg gacgctccgc gcgtttcatt
      601 gacggattcg cgccgcttat tgaaaactta tccggctgcg gcgcgtagca ctgttaaatt
      661 tattgtttac tctttaggtt tttatttatt taaacaatat gaaacatata cgcgttcttt
      721 tcgcgctggc ggcattattt attttttcgg cgtcggcgtc ctttgcccag acaaatggga
      781 aaatcagcgg caaggtaact tacggcggcg acaacacgat tctgcacgac gcggcggtgc
      841 agattgtgca gatgaagcgg acgatttcga ccgatgccgc cggcaattac gaatttacgg
      901 acattccgcc cggtcgctac acgcttctgg tgcacatcga gggctttgcc gacgcgacga
      961 aaattgtcga tttaaaagcc ggcgcgagcc agacggctga tttcgagctg caaatctcgt
     1021 cggttcgcgc cgacgtgacg gtgacggctt ccggcacgga gcaatcggtt ttcgattcgt
     1081 ttcaatcggt taacgcgctc agctcgacgc agattacgca gagggcttcg acctcgctcg
     1141 gcgaggttct ggagagcgaa accggcgtcg cgaaacgcag cttcggcgtc ggctcgtcgc
     1201 gccccgttat tcgcggattc gacggcgata gagtgctggt tttgcaggac ggcgtgcgcg
     1261 gcggctcgct cggctcgcag tcgggcgacc acggcgaatc ggtcgatccg ctcagcgccg
     1321 agcgcatcga ggtcgtcaaa ggtcccggca ctttgcttta cggcagcaac gcgctcggcg
     1381 gcgtcgtcaa cgtcatcggc aacgacgaga acgaagctca cgcgggcttt cgcggatttt
     1441 ttaccgcgct cgccggaacg gctgaccggc agggcgggct cgccggcgga gtcgaatacg
     1501 gttttaaaaa ctggctcttt cgcggaaacc tgagcgcgca gcggtcggac aattacgcga
     1561 cgccgctcgg catcgttccg aacagcgctt cgcgttccaa cacgggctct ttcggcgtcg
     1621 gttattacgg cgataaagcc tttttaagcg gaactttcaa cgtcgatatt cgccgttacg
     1681 gaattccttt cgcagcgctt tttgaaggcg gcgagaaagc tgcgccaaac tcgctcggcg
     1741 gcgagattcc gtttgttgac gaagacatag atattcgtat gcgacgccgc aattttcgcg
     1801 ttaacggcgg ttttcgcaat ctgaacaatt cttttctgag cagcattaat tacaatctcg
     1861 attacacgaa ttatcgccac aaggaaatcg aaatcgccga cggcatcgaa gaagtcggca
     1921 cgattttcga caacaagact ttctcgtatc gttccgtttt tgaacagacg aaatacaatc
     1981 gcctgaccgg acgcttcggt tttgaaggct ttgaccgcga ttacgaaatc aacggcgcgg
     2041 agcagttgat tcagggcaaa gtcaagcaca attcgattgc ctttttcggt ctggaagagc
     2101 tggatttcga gcgcgtcaag tttcaattcg gcgggcgcgt cgagcgcaac ggctatcgtc
     2161 ccgaaaatcc ggatttgctc gaacgcacat ttacgggctt ttcaggcggc gcgggcgtca
     2221 atttcagact gtggaaaggc ggcgcgttta tcgccaatta cacgcattcg tttcgcgcgc
     2281 cgtcgctcga agaactctat aacaacggtc cgcacgtcgg cacgatttcc tttgaaatcg
     2341 gcaacgaaaa cctgcgcccc gagcgctcga acggtctgga tttttcgctc cgtcatttgt
     2401 cagaccgctt tcgtatcatg ggcgatgttt attattaccg cataaataat ttcgtctttc
     2461 aggcattgca ggacgatgac ggcgacgggc aggtcgatat tatggaaggt cttcccgtgc
     2521 ggcgttacga gcagcaggac agccgctatc tgggcgcgga aatcagcgcc gacgtgacga
     2581 ttaataaata tctcgtcttt ctggcgagcg gcgatttggt caaagccgaa ttgacggacg
     2641 gcacgccgct gccgcgaatt ccgccgatgc gcggtcggct cggactcgac attcgataca
     2701 aaggcttgag cgtccgcccc gaaggcgttt ttgtcggcga gcgcgggcag gacgacattt
     2761 ttccgctcga aacgccgacc gacggatact cgctctttaa cgtcgccggt tcttacacca
     2821 tcggcagaca gcacttcgcg catattttca gctttaacgc gtacaatctg acgaacaagc
     2881 tgtatcgcaa tcacctgtcg tttatcaaag acctggcgcc ggaaatcggg cgcggaatcc
     2941 gtttcagcta cacggtcaga tttttctaaa acgagcaaag gatttggaag cgagcaaagg
     3001 gttggtctaa acggcgaatg tcggaatcga ttgaatcgat tccgacattc gccgttttgc
     3061 gacggctggg aaaattttac gaaatttact gcggcgaagc ggcaaccgga atgtcgccgt
     3121 tcgcgccgaa ctgattggct tgaagctgtc cgtttgagct tcgcagaatg aaataagttc
     3181 ctgacgaagg acgccagacc gccaaatccg atttgccgtc cctgtcgtaa tccgccatca
     3241 cgggcttgtc gccgcttgtg ccgaactgcg tcgccgcaaa ctgcccgtcg ctgcttctca
     3301 ggacaaacca ggttccgata gaaggtcgga aaacgataaa atcgttgcgc gaatcgccgt
     3361 ccacgtcgcc gttaaccggc aggtcgccgc tcgcgccgaa atgcaagccg gtgaaagtat
     3421 tcgaaccgct gttcaggaca aaccagtcgc ccgcgcccgg acgaaaaacg gctaaatccg
     3481 ccgtgccgtc gccgtcgaaa tccgattcgt gatgctgcgc cgcgcgcggc tcgccgtaga
     3541 tgaaatcgtc catcgccgcg acatcgacgc cgtcgagttc atcattgttg gtcgcggaaa
     3601 gcgccgcgtt gccgctttca atcacgacgc gcgcgatacg ctcgccgtta ttgaacgaga
     3661 tgccgacaaa cgaaagcccg ccatcgtgaa ccggcgcgct ggcggctgac aactgcgagc
     3721 cgtccgtgcc gtaaacgcga atcaagctgc gattgccgcc cgtcgccgaa tcgacgtccg
     3781 cgaaaacgac gccgaagccg ctgaccgtcg ccggaatctt cgtgccggga acaaagaaac
     3841 tgacttccag aatatgcgta ttgcgggcga taaataatcg ctgggcgctg aaagtctgga
     3901 aaatattagt ataagcggcg ttcagattgc cgaagcgaac cggcacgccc gatgccgtgt
     3961 tcgagctgac ggcaaactga ttcaaagccg cgccggtcgc gtcttcgatg gaattaaaaa
     4021 tcactccgcg cggactgttg acgttaaaaa agttcggcgg aaagttgttc ggctcggcga
     4081 aattgtccgg cacgccgtcc cagttgactt cgcgtctgcc ggacgtgaac gagccgccga
     4141 cgccgttgtt cgagccgccc aaatccgcgc gaaacaggtc gacgatcgct tgcagcccag
     4201 ccgcgttcgc gcccgcgccc tgacggacga ccggcgcggc aaaattgtta accgtcaaag
     4261 ccagaagcag catgacgagg gtaaaagcta aaaagtaaaa attgcgtttc ataatttaaa
     4321 atctccagat aatatttaat tttcatcttc aaaaaagatg attgacgatg tttaatagcg
     4381 aattcagaca aatcgcagat tcgtccgacg aagtttgata aaggcggatt ttgccttgcc
     4441 cgataaaact gcagattgtt tccggctcga ttccggcagc tttcgctgca tcttcaagcg
     4501 ttaaaatccc ggcttcggag cagcagcacg cgcaccattt caaatatctg ttgcggcggt
     4561 tgtaaatcac cctgactcgc ccgggcttca atgatgattt cccgtttcaa acaatttttc
     4621 ggttccctct ttcccgaagt tgcgttaaaa ttttttataa tcgagcgact tcggggtttt
     4681 gtccgctgaa accattaaag ccgaagaggg tcgcccgaat ctttagaaag ttctgaaatt
     4741 ccgctgaaaa aaacctgaaa gttttatgaa aaagttatga gagctttcgc ccgtaagatt
     4801 tacgaattcg gtcggtttcg cgtcgacgcc gtcgagcgcc tgctgtttga cggcgcaaaa
     4861 ccgctcgcgc tgacgccgaa agccttcgat ac
//
LOCUS       DASWNE010000304         2213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_574728, whole genome shotgun sequence.
ACCESSION   DASWNE010000304 DASWNE010000000
VERSION     DASWNE010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574728"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..280)
                     /locus_tag="VGB68_06305"
     CDS             complement(128..280)
                     /locus_tag="VGB68_06305"
                     /inference="COORDINATES: protein motif:HMM:NF033488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEY0048875.1"
                     /translation="MLWTILIVLLVLWALGFGFGGAAVGNLIHILLVVALVVLILQLV
                     RGRRGV"
     gene            543..>2213
                     /locus_tag="VGB68_06310"
     CDS             543..>2213
                     /locus_tag="VGB68_06310"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEY0048876.1"
                     /translation="MAENQPKRVFLIDTMSHIFRAFFAPMGARTEPLRNSRGQVTQAV
                     FVFTNMLRKLLKDENPEYIAAVFDSAEKTFRHDSYAEYKANRADMPDDLSSQMPYIVK
                     VCEAFNLPILKMDGFEADDIIGTLAQKVAAKGMQAVIVSNDKDLCQLVQDPLIVCMRQ
                     NSQNVKRKVAVPPVEWCDEAWVYNKFGVQPSQIVDLLGLMGDAIDNIPGAPGIGEKGA
                     MKIIQQFGSAEAAMQRADEVTHKTYRESLQNNQDIIRQSIELATVHLSVPVELDLDLL
                     KSREPNRQLAYQLFRELEFTSLTREFTDSAPLFDQLESAVLGIAERRYNIIRRREDLD
                     KLIRKLWESEHWTFETDDSNSPKSAASYQKAEPCGVAIATGAGASVYIDLENFEGGKE
                     QAVEPLKDILSNGFLAKGTHDLKRNLAVLKTVGIEPEAVEDDTLLAAYLLDSSRASYS
                     LPLLAMQNLGTDAEDEIPEGWTEAQYRTAERADFTYQLAGMLRRKLQEDGLEKIYTDI
                     ELPLVPLLYRMEAAGMKVDTDVLKGLSDYISTEVETLGAKIYELAGREF"
BASE COUNT          581 a          568 c          562 g          502 t
ORIGIN      
        1 gtcgcagagg agtttaaacc ggtcgcgtat ttctcccaat taaattaaat acataaaatg
       61 agcggcgttg tcgacaacgc cgctcttttt atgtatttaa tttaattggg agaaatacgc
      121 gaccggttta aactcctctg cgaccgcgaa ccaactgcag gattaaaacc actaaagcca
      181 ccaccagcag gatatgtatc aaattaccga cagccgcgcc tccaaagcca aaacctaatg
      241 cccacaaaac aagcagtaca attaagattg tccaaagcat aaatttttct ccttttgttc
      301 cctttttacg gataattcaa atatctcaat cacagatgcg taataaatcc atacattgag
      361 ttatttgttt tttttaaaag caataactat gccgcttcga aaggagaaag gctgaaggct
      421 gaaggcgaaa gtaaaacaag aaatttatcc tcattcttta tcctttcccc ttcagccttt
      481 ctcctttcga acgcgcgctg tgtggtaaaa ttatttaacc aaaataaatt acaaggtaaa
      541 taatggcaga aaatcaaccc aaacgagtct ttctgattga cacgatgtcg catattttcc
      601 gcgctttttt cgcgccgatg ggcgcgcgca ccgagccgct gcgcaactcg cgcgggcaag
      661 tcacgcaggc ggtgttcgtt tttacgaata tgctgcgcaa gcttttgaag gacgaaaatc
      721 ccgaatacat cgccgccgtt ttcgattcgg cggaaaaaac tttccggcac gattcctacg
      781 ccgaatacaa ggcgaaccgc gccgatatgc ccgacgattt gtcgtcgcag atgccttata
      841 tcgtcaaggt ctgcgaagcc tttaatcttc cgattctgaa aatggacggt tttgaagcgg
      901 acgatattat cgggactctg gcgcagaaag tcgccgccaa aggaatgcaa gccgtcatcg
      961 tctcgaacga caaggattta tgccagctcg tgcaagaccc attaatcgtc tgtatgcggc
     1021 agaattcgca gaacgtcaaa cgcaaagtcg ccgtgccgcc cgtcgaatgg tgcgacgagg
     1081 cttgggttta caataaattc ggcgttcagc cgtcgcaaat cgtcgacctt ctgggtttga
     1141 tgggcgacgc gattgacaac attcccggcg ctcccggcat cggcgaaaaa ggcgcgatga
     1201 aaatcatcca gcaattcggg tcggcggaag cggcgatgca gcgcgccgac gaagtgacgc
     1261 ataaaacata ccgtgaaagc ctgcaaaaca atcaggatat tattcgccag tcaatcgagc
     1321 tggcgacggt tcacttgagc gttccggtcg agctggattt ggatttatta aaaagccgcg
     1381 agccgaatcg ccagcttgcc taccagcttt tccgcgagct ggaatttacg tctctgacgc
     1441 gcgagtttac cgattccgcg ccgcttttcg accagctcga atccgccgtc ctcggcattg
     1501 ccgagcgccg ctacaatatc ataaggcggc gcgaggattt ggataagctc atcaggaaat
     1561 tgtgggaaag cgagcactgg actttcgaga cggacgattc gaattcgccg aaaagcgcgg
     1621 cttcgtatca aaaagccgag ccgtgcggcg tggcgattgc gaccggcgcg ggcgcgagcg
     1681 tctacattga tttggaaaat ttcgaaggcg gcaaagagca ggcggtcgag cctttaaaag
     1741 acatcctttc gaacggtttt ctggcgaaag gcacgcacga tttaaaacgc aatctcgcgg
     1801 ttctgaaaac cgtcggcatc gagccggaag ccgtcgaaga cgacacgctg ctcgccgctt
     1861 atctgctgga ttcgagccgc gccagttatt cgctgccgct gctggcgatg cagaatctcg
     1921 gaaccgacgc cgaagacgaa atccccgaag gctggaccga agcgcagtat cgcacggcgg
     1981 aacgcgccga ttttacgtat cagcttgccg gaatgctccg ccgcaaattg caggaagacg
     2041 gtctggaaaa aatttacacc gatatcgagc tgccgctcgt gccgctgctt taccggatgg
     2101 aagcggcggg aatgaaggtc gacacggacg ttctgaaagg tctttcggat tacatctcga
     2161 cggaagtcga aactttgggc gcgaagattt acgaacttgc cgggcgcgag ttt
//
LOCUS       DASWNE010000305         2573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_645142, whole genome shotgun sequence.
ACCESSION   DASWNE010000305 DASWNE010000000
VERSION     DASWNE010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645142"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VGB68_06315"
     CDS             complement(<1..590)
                     /locus_tag="VGB68_06315"
                     /inference="COORDINATES: protein motif:HMM:NF014345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HEY0048877.1"
                     /translation="MKFFVKSVLCLLTLSSLFAVDVFACTTFCLKRGSEVLFGKNYDW
                     MIGDGLIFVNRRGVAKTAMSGGTNSAGWTSRYGSITFNQYGRENPSGGMNEAGLVVEL
                     IWLDDTSYPAADLRPTVDTLEWIQYQLDNFASVDEVVKASEYLRISSFVKLHYLVGDR
                     NGNSSSIEFLNGKLVVHAGDKMPVSALANDTYEKSWA"
     gene            complement(605..1054)
                     /locus_tag="VGB68_06320"
     CDS             complement(605..1054)
                     /locus_tag="VGB68_06320"
                     /inference="COORDINATES: protein motif:HMM:NF028004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HEY0048878.1"
                     /translation="MPLSSSGNSTPVPTVTSSKGTTRIIMPNDRMPAPKEKTQKNPHE
                     KGNQTFARVFANEPASQEINNDEPHFAAGSIIVREKLLRETDTTPEIVTVMIKREKGF
                     SPKTGDWEFFVLEGTLEKVEKRETVGSCAKCHAQAAKSDWVFKTYLK"
     gene            complement(1079..1315)
                     /locus_tag="VGB68_06325"
     CDS             complement(1079..1315)
                     /locus_tag="VGB68_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048879.1"
                     /translation="MKVKLLKIFIVGAFSAFAACVFFISGNGKIEAQSREARKKKAEI
                     LEKAAGYKAWKQVVKPAPQASPQIISIPDSSSYG"
     gene            complement(1649..>2573)
                     /gene="lipA"
                     /locus_tag="VGB68_06330"
     CDS             complement(1649..>2573)
                     /gene="lipA"
                     /locus_tag="VGB68_06330"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEY0048880.1"
                     /translation="QKFVKKKKERLPKPDWLRIKLGDPTNQNAVLKLIEGLNLHTVCQ
                     EARCPNIFECWTDRTATFMLGGDTCTRHCGFCAVNKGKPMDLDPMEPTHVAEAVAHLN
                     LAHAVITSVNRDDLPDGGATHWAETILKVRELNPSCKVEVLIPDFNGDEAALNTVLQA
                     RPDVLNHNTETIARLYRRVRPDAKYAQTLELLERSARWRDAQQPAMKTKSGIMVGLGE
                     EFEEVVELMKDLRSVSCDIMTIGQYLQPYERRLPVERYVTPEEFAEWKRIGKEMGFKH
                     IESSPLTRSSYHARQQTESGEQSAALVQIAT"
BASE COUNT          536 a          682 c          667 g          688 t
ORIGIN      
        1 gcccaggatt tttcgtatgt gtcgtttgcg agcgccgaaa cgggcatttt atcgcccgcg
       61 tgtacgacga gttttccgtt taaaaattcg atgctcgacg aatttccgtt acggtcgccg
      121 accagataat gcagcttgac gaacgacgag atgcgcaagt attcgctggc tttgacgact
      181 tcatcgacgc tcgcgaaatt gtcgagctga tactgaatcc attcgagcgt gtcaacggtc
      241 ggacgcaaat ccgccgccgg ataactcgtg tcgtcgagcc aaatcagttc gacgaccaga
      301 ccggcttcgt tcattccgcc cgaaggattt tcgcgcccgt attgattaaa agtgatgctt
      361 ccgtaacgcg aagtccatcc ggcggaattt gtgccgccgg acatcgccgt tttcgccacg
      421 ccgcgccggt tgacgaaaat cagcccgtcg ccaatcatcc aatcgtagtt tttgccgaac
      481 agaacctcgc tgccgcgttt caggcaaaac gtcgtgcagg cgaaaacgtc aaccgcgaaa
      541 agcgacgaca aagttaataa acacaagacg cttttgacaa aaaatttcat aatccaaact
      601 cgcgttattt cagatacgtt ttaaaaaccc aatcgctttt cgcggcttgc gcgtggcatt
      661 tggcgcagct gccgacggtt tcgcgttttt ccactttttc gagcgttcct tccagcacaa
      721 aaaattccca gtcgccggtt ttcgggctga aacctttttc gcgttttatc attacggtga
      781 cgatttcggg cgtcgtgtcg gtctcgcgca aaagcttttc gcggacgata atcgaaccgg
      841 cggcaaaatg cggctcgtcg ttgttgatct cctgcgaagc cggctcgttg gcaaaaacgc
      901 gtgcgaatgt ttgattgccc ttttcgtgcg gatttttctg cgttttttct ttcggcgcgg
      961 gcatccggtc gttcggcata atgatgcgcg tcgttccttt cgaactcgtc acggtcggca
     1021 ccggcgtgct gttgccgctc gaactgagcg gcaaatcgac tccgtcgatt ataaaagttc
     1081 agccgtagct cgaagaatcg ggaatcgaaa taatctgcgg gctcgcctgt ggcgcgggtt
     1141 tgacaacctg tttccacgct ttataaccgg ctgctttttc cagaatctcg gcttttttct
     1201 ttctcgcctc gcggctctga gcttcgattt tgccgttgcc ggaaataaaa aagacgcagg
     1261 cggcaaaagc cgaaaatgcg ccgacaataa aaatttttaa aagcttcact ttcatactgt
     1321 tgtttcccgg tatgccggaa ttatctgccc gcgctgcctg attttgtaag ggtggtcaaa
     1381 taaattgatg cgccggcgct tttggcggtt gcttcgacgt aactcgcaga taatattcga
     1441 aaatgcaggt cttaacagga aaaatcggct taacctttcg attctgccga ttttcgagat
     1501 ttttgataat tagtaggttt cgacgccgtt cgaaacagat gcgaaattcc tggtttcgcg
     1561 ccgcatttca taaagcttga gaatcagctc ggcgtccgcc ggttttgttc tcattgtcgt
     1621 ttcttctttg atagaagttt atatattttt aagtcgctat ttgaacgagc gcggcgcttt
     1681 gctcgccgga ctcggtttgc tggcgcgcgt gatacgacga gcgcgtcagc ggcgacgact
     1741 cgatgtgttt gaagcccatt tccttgccga ttcgtttcca ttcggcaaat tcttcgggcg
     1801 tcacgtatct ctcgaccgga agccgcctct cgtaaggctg tagatattgc ccgatagtca
     1861 taatgtcgca ggaaaccgag cgcaaatcct tcatcaattc gacgacttct tcaaactctt
     1921 cgccgagccc gaccataatt ccgcttttcg tcttcatcgc gggctgctgc gcgtcgcgcc
     1981 agcgtgccga gcgttccagt aattccagag tctgcgcgta tttcgcgtcg ggacggacgc
     2041 ggcgataaag ccgggcgatg gtttccgtgt tgtgattcaa aacgtcgggg cgcgcctgca
     2101 aaaccgtgtt cagcgccgct tcgtcgccgt taaaatcggg aatcagaact tcgaccttgc
     2161 agctcggatt cagctcgcga actttcagaa tcgtttccgc ccagtgcgtc gcgccgccgt
     2221 cgggcaaatc gtcgcgattg accgaagtga taacggcgtg agcgagattt aaatgcgcta
     2281 cggcttcggc gacgtgcgtc ggctccatcg ggtccaagtc catcggtttg cctttattga
     2341 cggcgcaaaa gccgcaatga cgcgtgcagg tgtcgccgcc gagcataaag gtcgccgtgc
     2401 ggtcggtcca gcattcgaag atgttcgggc agcgcgcttc ttggcaaacc gtgtgcagat
     2461 ttaagccttc aatcaatttt aaaacggcgt tttgattggt cgggtcgccg agcttgatac
     2521 gcagccaatc gggcttcggc aggcgctctt ttttcttttt gacaaatttc tgg
//
LOCUS       DASWNE010000306         1567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_10106, whole genome shotgun sequence.
ACCESSION   DASWNE010000306 DASWNE010000000
VERSION     DASWNE010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10106"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1563
                     /gene="groL"
                     /locus_tag="VGB68_06335"
     CDS             <1..1563
                     /gene="groL"
                     /locus_tag="VGB68_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211302.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY0048881.1"
                     /translation="DILANAVGVTLGPKGRNVVIGRIYGSPLITKDGVTVAKEIELPD
                     AYQNMGAQMVKEVATKTNDIAGDGTTTATVLAQAIFREGVKNVAAGANPMSLQRGIQM
                     ATERAVEAIKNQSQNVAGQSGFANVASVSANNDRNIGELIAAAMEQVGKDGVVTVEES
                     KTMATELDLVEGMQFDRGYLSAYLVTNADRMETVLDEPLILIYEKKIASMRELIPVLE
                     LSAQQGRSLLIIAEDVEAEALATLVVNKLRGTIKVCAVKAPAFGDRRKEILEDIAVLT
                     GGRVITEDIGIKLESVTQDDLGTAKRVIVDKDSTTIVEGAGQASAIEGRIQTIRSQTE
                     NVSSDYEREKLQERLAKLAGGVAVVRVGAATETEMKEKKARIEDALNATKAAAQEGIV
                     AGGGVALLRASKELDSIESDDRDFLTGVKIVKRAMEEPLHRIARNAGIDGAIVVEKVL
                     AAEGGNGFNAATGNYEDLLAAGIIDPAKVVRTALQNAASIAGLMLTTDATIVDAPEKK
                     KAAAGTHGEDYD"
BASE COUNT          392 a          412 c          500 g          263 t
ORIGIN      
        1 gatattctgg caaacgcggt cggcgtgacc ttgggtccga aggggcgcaa tgtcgtgatt
       61 ggtcgtattt acggctcgcc tctaatcacc aaagacggcg tgacggtcgc taaagaaatc
      121 gaactgcccg acgcttatca gaatatgggc gcgcagatgg tgaaggaagt ggcgacgaaa
      181 accaacgaca ttgccggcga cggcacgacg acggcaaccg ttctggcgca ggcgattttc
      241 cgcgagggcg taaaaaacgt ggcggcgggc gccaacccaa tgtctttgca gcgcggaatc
      301 cagatggcga ccgagcgcgc ggtcgaagcc atcaaaaatc aatcgcaaaa tgtcgcggga
      361 caaagcggat ttgccaacgt cgcatcggtt tccgccaaca atgaccgcaa tatcggcgag
      421 cttatcgccg ccgcgatgga acaggtcggc aaagacggcg tcgtgacggt cgaagaatcg
      481 aaaacgatgg cgaccgagct tgacctggtc gagggaatgc agtttgaccg cggttatctt
      541 tcggcttatc tggtgacgaa cgccgaccgt atggaaacgg ttctggacga gccgctcatc
      601 ctgatttacg aaaagaaaat cgcttcgatg cgcgaattga ttccggttct cgaactgtcg
      661 gcgcagcagg ggcgctcgct tttgattatc gccgaggacg tggaagccga agccctggcg
      721 acgctggtcg tcaacaaact gcgcggcaca attaaagttt gcgccgtcaa ggctcccgca
      781 ttcggcgacc gccgcaagga aattctcgaa gacatcgccg ttttgaccgg cggaagagtt
      841 atcaccgaag acatcggcat caagcttgaa agcgtgacgc aggacgattt gggaacggca
      901 aagcgcgtca ttgtcgataa agacagcacg acgattgtcg aaggcgcggg acaagcaagc
      961 gcgattgaag gacgcatcca aaccatccgg agccagaccg aaaacgtctc cagcgattat
     1021 gaacgcgaaa aattgcagga gcggctggca aagctcgcgg gcggcgtcgc ggtcgtgcgc
     1081 gtcggcgcgg cgaccgaaac cgagatgaag gagaaaaaag cgcgtatcga agacgctctg
     1141 aacgcgacca aagcggcggc gcaggaaggc atcgtggcgg gcggcggcgt ggctttgctg
     1201 cgagcctcga aagaattgga ctcgattgag tccgacgacc gggactttct gacgggcgta
     1261 aaaatcgtca aacgcgcgat ggaagaaccg ctccatcgca tcgcccgaaa cgccggaatc
     1321 gacggcgcta tcgtcgtcga aaaagttctg gctgcggaag gcggaaacgg ctttaacgcg
     1381 gcgacgggca attatgaaga cttgctggcg gcgggcatta tcgacccggc gaaagtcgtg
     1441 cggacggctt tgcagaacgc ggcttcgatt gccggcttga tgctgacgac cgacgccacg
     1501 atagtcgacg cgccggagaa gaaaaaagcc gctgccggaa cgcacggcga ggattatgac
     1561 tagaagt
//
LOCUS       DASWNE010000307         2836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_362035, whole genome shotgun sequence.
ACCESSION   DASWNE010000307 DASWNE010000000
VERSION     DASWNE010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362035"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VGB68_06340"
     CDS             <1..556
                     /locus_tag="VGB68_06340"
                     /inference="COORDINATES: protein motif:HMM:NF012859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HEY0048882.1"
                     /translation="WQATDYDHEFYVNMGWGSSSPGYGRKWIAGKTWFTGQLYPNRPT
                     ETKKLIAKHSNKCLDVYNSGGGDGTNVIQWNCHGGANQQWRLEPVFTVGNYYRILARH
                     SGKALDVSGISTATGANVHQWEWWMGNNQLWELIPSGGGYFMLKARHSGKCLDVSGVS
                     TADGANVYQWDCHGGANQLWRIVP"
     gene            883..1734
                     /locus_tag="VGB68_06345"
     CDS             883..1734
                     /locus_tag="VGB68_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048883.1"
                     /translation="MKSSNSKIYKVIFAAAVLFASAIFCFGQRELTPLEFVGASDENP
                     AVQSVTVQADPDATVEVSDERLIRAELVREGETSRLDVSVNTFGMEGSEEGTAYEGRI
                     HIGGSNPFIIPIKIIIFWFTPKECRGKRVSVQYLSRGTADNFVGAEAAFPSPYQLATY
                     PIQRRYDDPGDDRYFGDSYRLGSCRVCAVRVYVRARGEGGLDYNDSLWFQVSDAANGT
                     NYNPVNIAPGFTNIWNSSSAPFTFYREIPGSVINPELWGKNAATLDITAQDDTVVDWT
                     RVYIYKY"
     gene            2067..>2836
                     /locus_tag="VGB68_06350"
     CDS             2067..>2836
                     /locus_tag="VGB68_06350"
                     /inference="COORDINATES: protein motif:HMM:NF019145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048884.1"
                     /translation="MIAALFFNLLPISVRAQKTSDVPPSSPDYERIHKLSLEFLDGEL
                     DRYTPEPKNTHFKALSMQQDELGLYHTKVQQFYNDVPVWGSEAIVHLNRDESLYTITN
                     DLVVELEEMRIDTAPQIAVEEAVKRAFELEGCTECRLRADTRPELWLVAEERDLQKEN
                     PEEYAETKVNLAYRIQLEQWGDGNERSELKMPVYFIDAQTGKQVLSYDNLQTQSVTNS
                     GSSLYSGTVNFTAFRFGTPYYLENLNRRIGTFNGNTATR"
BASE COUNT          783 a          690 c          708 g          655 t
ORIGIN      
        1 ctggcaggcg accgattacg accacgaatt ctacgtcaat atgggctggg gcagctcctc
       61 gcccggctac ggaagaaagt ggattgcggg caaaacgtgg tttaccggtc agctctaccc
      121 gaatcgcccg accgagacca aaaaactgat tgccaaacac agcaacaaat gtctggacgt
      181 ttacaattcg ggcggcggag acggcacaaa cgttatccag tggaactgtc acggcggagc
      241 taaccagcag tggagactcg aacccgtttt caccgtcggc aattactatc gcattctggc
      301 gcgccacagc ggaaaggcgc tggacgtttc gggaatttca accgccaccg gagcgaacgt
      361 tcatcaatgg gaatggtgga tgggcaacaa ccagctctgg gaactgattc cgagcggcgg
      421 aggatatttt atgctgaaag ccagacatag cggcaaatgt ctcgacgttt cgggagtttc
      481 gaccgccgac ggagcaaacg tctatcaatg ggattgtcac ggcggcgcta accagttgtg
      541 gagaatcgtg ccttagcgta gaagctttct tcgcttaata aacgggtcgt gggagcgact
      601 ttattcgacg gaataggtcg ctcccggctc gtaaatcgac gaataattat tcttttttaa
      661 agagcgaaac ttttttggaa ttttgtctat tagaaatagc aggtaaataa attacttcgt
      721 cttaacaaac caaaagcctg acgcatcggc tatgactatc aggctttcgg acaggtactc
      781 tttgtctgtt ttcgcagata gaatactgct ttttattcta tctgcgaaac gtcagagact
      841 caaccccttt gggagacttc aataaaaaag gagctaaaaa aaatgaaaag cagcaactca
      901 aaaatttata aagttatttt cgccgcagcc gttttgttcg cttcggcgat tttctgtttc
      961 gggcagcgcg agctgacgcc gctggaattt gtcggcgcga gcgacgaaaa tcccgccgtt
     1021 caatccgtaa ccgtgcaggc tgatccggat gcgacggtcg aagtcagcga cgaaagattg
     1081 attcgcgccg agctggtaag agaaggcgaa acgtcgcggc tcgacgtctc ggtaaatacc
     1141 ttcggaatgg aaggcagcga agaaggcacg gcttatgaag gaagaatcca catcggcggt
     1201 tcgaatccct ttataatccc gattaaaatt attattttct ggtttacgcc gaaagaatgt
     1261 cgcggcaagc gcgtcagcgt ccaatatctg agcagaggaa cggcggataa tttcgtcggc
     1321 gcggaagcgg cttttccgag cccgtatcag ttagcgacct atccgataca aagacgctat
     1381 gacgaccccg gagacgacag atatttcggc gacagttatc gactcgggag ctgccgcgtc
     1441 tgcgccgtca gagtttatgt ccgcgccaga ggagaaggcg gtcttgacta taacgacagc
     1501 ctgtggtttc aggtcagcga cgccgcaaac ggaacgaact acaatcccgt caatatcgct
     1561 cccggcttca ccaacatatg gaacagctct tctgcgccct ttacgtttta tcgcgaaatt
     1621 ccgggctcgg tcattaatcc ggagctttgg ggtaaaaacg ccgcgacgct cgacatcacg
     1681 gcacaggacg atacggtagt cgactggacg agagtttata tctacaaata ttaaactcgc
     1741 cgtcattcgt aaacccgtca gccgaaaagg aagcgaaatt cgcttccttt tttttgttaa
     1801 aaacttttaa tgaaaccttt ttaaatttcg gtctattata aatagaacca aaaaactgtc
     1861 agttttctca actctacaac tcaacaatcg cgcaccggct actgcattta tcaggagcac
     1921 atcgaacgaa gtttctctat ttttacaaat aaggactccg actccgcttt cttcggctaa
     1981 acagaaaaga tgcgctgact tagaaaaaaa taaaatttaa ggagaatatc atcgtgaaaa
     2041 aaacggtttc cgcaacagca gtatttttga ttgctgctct attttttaat cttctgccaa
     2101 tctctgtccg cgcgcagaaa acgtcggatg ttccgccgtc gtcgcctgat tatgaaagaa
     2161 tccataaact cagtctggag tttctggacg gcgagcttga tagatacacg cccgagccga
     2221 aaaacacaca tttcaaagct ctgtcaatgc agcaggacga actgggactg tatcacacca
     2281 aagtgcagca gttttacaac gacgttccgg tttggggcag cgaagccatc gtgcacctga
     2341 atcgtgacga atcgctttac acgattacca acgacctcgt cgtcgaactg gaagaaatgc
     2401 ggattgacac cgccccgcaa attgcggtcg aagaagcggt taaaagagct ttcgagctgg
     2461 aaggatgcac ggagtgccgc ctgcgcgccg atacccgacc cgaattatgg ctggtcgccg
     2521 aagaacgcga tttgcaaaag gaaaatccgg aagaatatgc cgaaacgaag gtcaatctgg
     2581 cgtatcgcat ccaattagag caatggggcg acggaaatga aagaagcgag ctgaaaatgc
     2641 ccgtgtattt tatcgacgcg cagacgggca aacaagttct cagctacgat aatctgcaaa
     2701 cgcagagcgt gacgaactcc ggttcgtctt tatacagcgg aacggtcaat tttacggcgt
     2761 ttcgtttcgg cactccgtat tatctggaaa atctgaatcg ccgcatcgga acctttaacg
     2821 gaaacacggc gacgcg
//
LOCUS       DASWNE010000308         3058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_574892, whole genome shotgun sequence.
ACCESSION   DASWNE010000308 DASWNE010000000
VERSION     DASWNE010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574892"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(466..1254)
                     /locus_tag="VGB68_06355"
     CDS             complement(466..1254)
                     /locus_tag="VGB68_06355"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY0048885.1"
                     /translation="MIKPLKILIVDDEPLARGIIRRMLETDSRIQSIAEAEDGDEALE
                     TIRSNAPDIVYLDIQMPGANGFELLDQIRQLKSAAMPLIIFVTAYDEYALEAFEFYAL
                     DYLVKPVDPARFRQTFDIAVKQLSAGDEAEYQQKLFQIVSRMEPQKNYLEWVSVKTGG
                     GISLLKIEKIRWIEAQGNYALLDFGNNLTHLMREQIGTLESKLDPQTFIRIHRSTIIN
                     INFIKEIENWGRGEFKVAMSENKVFTVGHSFRANFDDFLKKKVL"
     gene            complement(1298..2431)
                     /locus_tag="VGB68_06360"
     CDS             complement(1298..2431)
                     /locus_tag="VGB68_06360"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY0048886.1"
                     /translation="MSRAAEESESKERVKAEIPWKSVGIGVVVWSIIGFLFAFEFYLS
                     NYKSIKFISFWDVIGGFLREISFYAVFTPLLLKFGSAYPFEFQISIKRFFRNFFIHLV
                     AAFPFAIICFFLQYFVYGITRGEICLDCFKLEVLLNPNYVHRGVIVYWGIILVGQGVE
                     YFRRLNDEKVRVAQLSTQLSEAQLSALKMQIHPHFLFNTLNSIVGLIQTDRDSAEIMT
                     RKLSDFLRITLKNSGEMTVSLEQEFSFIKTYLDIEKVRFQKRLTIEFSFDEDILQAKV
                     PNLILQPLVENSIKHGISRRKKDGRIKISAFRERDFLVLEVLDNGDWSDKAHNGMEPE
                     KSGVGLKNTAERLKQIYGNEHFFSLINDAETDTVATIKIPYRV"
     gene            complement(2474..>3058)
                     /locus_tag="VGB68_06365"
     CDS             complement(2474..>3058)
                     /locus_tag="VGB68_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048887.1"
                     /translation="RSGNTKIWRMDIKGENLTQLTHGTASDYLPYHTPDGRMLVYTSS
                     DTRNQTLWKISLDANSSPIQLTRDNSAWGTVSPDGSLIACWQADEKTGKVKLAVISID
                     GGEAIKSFGVSPTAKTWADVRWTSDGRAVTYIDTIGGVGNIWQQPIAGDSPKQLTDFK
                     TDRIFRFAPSPDGKRMVYSRGVETNDVVLISNFR"
BASE COUNT          825 a          711 c          661 g          861 t
ORIGIN      
        1 cgaaaacgat aaaacgataa aacgataaaa cgaaaaacga aaaactttaa ttttcaaact
       61 gttcgttttc cgctttccgt ttcctttctg ctttgcacaa aaataaagaa aatcgagaaa
      121 aattccgatg gctaattgta aattctcaat tattaattct caattgcttc acaaaagata
      181 ccgaacatca atttagaatt tacaatttag aatttacaat tttccctttc tttatttttg
      241 tgcaaagcct cggtctgtaa ctttattatt ttttcccggc tttgatctct cccgctcttt
      301 gctgttgaat ttatgtagat tcatttcgga tattagcgct tttttggcaa tggatttata
      361 aatttgtgat taagcacgga tttttcggta taaatctctg aaaatctgaa ataaaggcgg
      421 aagcgattat ttatccgaaa aatcgagcgc agattttatt aaaggctaga gaactttttt
      481 tttgagaaaa tcgtcgaaat tggcgcgaaa gctgtgaccg accgtaaaga ctttattttc
      541 gctcatcgcg accttgaatt cgccgcgtcc ccaattttcg atttccttaa tgaaattgat
      601 atttataatg gtcgagcggt gaatgcgaat aaaggtctgc gggtcaagtt ttgattccag
      661 agtcccgatt tgttctctca tcagatgcgt taaattattg ccgaaatcga gcaaagcata
      721 gttgccctgc gcttcgatcc agcggatttt ctcgattttc aatagcgaga tgccgccgcc
      781 cgtcttgacg gaaacccatt ccagataatt tttttgaggc tccatacggc tgacaatctg
      841 aaacagtttc tgctgatatt ccgcttcgtc gcccgcggaa agctgtttta cggcgatatc
      901 gaaagtctgc cggaaccggg cgggatcgac gggcttgacc agataatcca aagcatagaa
      961 ttcaaaagct tcgagcgcgt attcgtcgta cgccgtcaca aagatgatga gcggcatcgc
     1021 cgccgatttc aattgccgaa tttggtcgag caattcgaag ccgttggctc ccggcatttg
     1081 gatatcgagg taaacaatat cgggcgcatt gctgcgaatc gtctcaagcg cctcgtcgcc
     1141 gtcttcggct tcggcgattg actgaatgcg ggaatctgtt tccagcattc gacgaataat
     1201 cccgcgcgcc agaggctcgt cgtcgacaat taaaattttc agcggtttta tcatcacaat
     1261 aaactttacg ggcaggtcac aaacctgacg ggcaaagcta aactcgatac ggaattttga
     1321 tcgtcgcgac cgtgtccgtt tctgcatcgt taattaacga gaaaaaatgc tcgtttccgt
     1381 aaatttgctt taatctttcg gcggtatttt ttaagccgac gccgcttttt tccggttcca
     1441 tcccgttatg agctttatcc gaccaatctc cgttatcgag aacttcgagc accaggaaat
     1501 cccgctcgcg aaacgccgag atttttatcc tgccgtcttt tttccggcgg ctgatgccgt
     1561 gtttgataga attttcgacg agcggctgta gaatcaaatt cggaactttg gcttgcagaa
     1621 tgtcttcatc aaagctgaat tcgattgtta atcttttctg aaaccgcact ttttcgatgt
     1681 cgagataagt tttaataaaa gaaaattcct gttcgagaga aaccgtcatt tcacccgaat
     1741 ttttcagcgt gattcgcaga aaatccgata atttccgggt cattatctcg gcggaatccc
     1801 tgtccgtctg aatcagcccg acgatcgagt tgagcgtatt aaacaaaaaa tgcgggtgaa
     1861 tctgcatttt cagcgcggaa agctgcgcct cggaaagctg cgtcgaaagc tgcgcgacgc
     1921 gaactttttc atcgttcaga cgacgaaaat attcgactcc ctgcccgaca agaataattc
     1981 cccaataaac aatgacgccg cgatgcacat aattgggatt gagcagaact tccagcttaa
     2041 agcaatccag acagatttct ccccgagtta ttccgtaaac gaaatactga agaaaaaagc
     2101 agattatcgc aaaagggaaa gcggcgacga gatgtataaa aaaattgcga aaaaaccgtt
     2161 tgattgaaat ctggaattca aacggataag cgcttccgaa ttttaataaa agcggcgtga
     2221 aaacggcgta aaaagaaatt tcccgcaaaa atccgccgat aacatcccaa aaagaaatga
     2281 atttgatcga cttgtaattg cttaaataaa actcgaaagc gaaaagaaat ccgataatgc
     2341 tccaaacgac aacgccgatt ccgacgcttt tccagggaat ttccgcttta accctttctt
     2401 tgctctcgct ttcttcagcg gctcgggaca ttgtaaaaaa ttctgttttc ttaatcggca
     2461 tcgttcgttc cggtcatctg aaattactga ttaacacgac gtcattcgtc tctacgccgc
     2521 gcgaatatac cattcgcttg ccgtccggcg acggtgcgaa gcgaaatatc cggtcggttt
     2581 taaaatcggt caattgtttg ggcgaatcgc ctgcaatcgg ctgctgccag atattaccga
     2641 ctcctcctat cgtatcgatg tatgtaacgg cgcgtccgtc ggaagtccag cgaacatccg
     2701 cccaggtttt cgcggtgggc gaaacgccga aagatttaat cgcctcgccg ccgtcgatac
     2761 tgatgaccgc cagcttaact tttcctgttt tctcatctgc ctgccagcag gcaatcaggc
     2821 tgccgtcggg cgaaaccgtg ccccaggcgg aattgtcacg cgttaactga atgggcgaac
     2881 tgtttgcgtc aagcgaaatc ttccacaaag tttgatttct cgtgtcgctc gaagtgtaaa
     2941 ccagcattcg cccgtccggc gtgtgataag gaaggtaatc ggacgcggtg ccatgagtta
     3001 attgcgtgag attttcgccc ttgatgtcca tccgccatat tttagtattg cccgagcg
//
LOCUS       DASWNE010000309         5054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_150947, whole genome shotgun sequence.
ACCESSION   DASWNE010000309 DASWNE010000000
VERSION     DASWNE010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5054
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150947"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VGB68_06370"
     CDS             complement(<1..429)
                     /locus_tag="VGB68_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0048888.1"
                     /translation="MKRIHSILLILILALFGSAFTKVFAQTPALAPTTAAQKTNSSLP
                     LTLPLQEGSVRFLAIGDTGRGNKEQNELAQVMFNYRQVFPYEFVIMTGDNIYYQEKAE
                     DLKTKFEDVYRPLLNDGVKFYAALGNHDESNQRFYEHFNMN"
     gene            complement(478..1035)
                     /locus_tag="VGB68_06375"
     CDS             complement(478..1035)
                     /locus_tag="VGB68_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048889.1"
                     /translation="MKSVKKYYISIIFLLLCSFVAHQTATAQKTLSPKDKNTTTEFEK
                     SAKEYSKLREQLEEKLPKLPKDATPEQIEAHKAAFQKLVRAARSSAKQGDIFTPAAAE
                     LIRTIIKSEFKGKDRAELRETVLEADTKGILLKINFPYPDSKEQTEMPPTLLLNFPQL
                     PKQLRFRFVGRNLLLLDRENGLIVD"
     gene            complement(2042..3004)
                     /locus_tag="VGB68_06380"
     CDS             complement(2042..3004)
                     /locus_tag="VGB68_06380"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEY0048890.1"
                     /translation="MSFNFIDVLLIFFILLSVLYGWHRGFILSLLDLLRWIGSVLAGL
                     HFYQPLARRLGLINDWTEVWNQPLAFVLIVAAVSILFQIVAYALLRRLPGDIHKRSVN
                     RLLGTLPGFVNGLILAAILSSMLFSMPFSDGLQEKTRESATANYLAGYTEELETALSS
                     IFGDAVQQTLNRRTIHPESNERVELPFKVSETRPRPEIEAQMLELVNRERLAAGLKPL
                     EADPELTEVARRHSADMFARGYFSHNTPENLDPFDRMRRAEVRFLTAGENLALAPTVQ
                     IAHTGLMNSPGHRANILRPQFGRVGIGIMDGGRRGLMVTQNFRN"
     gene            complement(3241..3813)
                     /locus_tag="VGB68_06385"
     CDS             complement(3241..3813)
                     /locus_tag="VGB68_06385"
                     /inference="COORDINATES: protein motif:HMM:NF016974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEY0048891.1"
                     /translation="MIPVLKLPFVFDSQRLKNDLRGFAAIDWTPHFNTRYYEGDWSGI
                     ALRAPRGSAVNQLYPDPTAAGGYADTEMLGRCAYIPEVLAAFRCELESARFLRLGAGA
                     NIREHRDYRLGYEDGFIRLHIPVQTNPQVEFFLDGQSVEMKEGEAWYLNFNLKHRVKN
                     GGASDRVHLVVDCVLNDWLRAFFPPEEQTL"
     gene            complement(3839..4897)
                     /locus_tag="VGB68_06390"
     CDS             complement(3839..4897)
                     /locus_tag="VGB68_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048892.1"
                     /translation="MNQTLENLRDWMPARVYWQENRPLVDWCYLGKKRFTEPFFDQTI
                     ERRFSEPFNLLFRHQTPIEILGELHAASSGIAPTGFIFHMSRCGSTLIAQMLAASAQN
                     IVVSEPPPVDSVLRSNFKNPEISDEQRIQWLRWIIGAFGQKRNAEEKYYFIKFDSWSA
                     FELALIESAFPGVPWIFLYRNPVEVIASHMKQRGAQMIPGVVEKILPGLDLPDILQMP
                     SEEYCARVLARICESALNRLPNRNALLVNYTQLPEAVTSAVLKHFRAEYAPEDIGQME
                     NAAQFNAKTPRMNFVPDSETKKKQASGAAHAAAERWVNPLYEKLETARRDFESRERKF
                     SRRRRTEITRNETADFDG"
BASE COUNT         1277 a         1217 c         1219 g         1341 t
ORIGIN      
        1 gttcatatta aaatgttcgt aaaatcgctg gttggattcg tcgtgattgc cgagcgcggc
       61 gtaaaactta acgccgtcgt tgagcagcgg tctgtagaca tcttcaaact ttgttttcag
      121 gtcttcggct ttttcctgat agtagatatt atcgcccgtc ataatgacaa attcgtaggg
      181 aaatacctgc cggtaattaa acatcacttg agccagctcg ttttgctctt tattacctct
      241 gcccgtgtcg ccgatggcga gaaagcgcac cgagccttcc tgaagcggta aagtcaaagg
      301 taaagacgaa tttgtttttt gagcggctgt ggttggggcg agagcgggag tttgcgcaaa
      361 aactttggta aatgccgagc cgaacagggc aagtatcaaa atcagcagaa tactatggat
      421 tcgtttcatc ctttttatcc tcctttatca agcggttacg ggagagcgtc ggtcatacta
      481 atcaactata agtccgtttt ctctgtctaa aagcagaaga ttgcgcccga caaaccgaaa
      541 gcgaagttgt ttcggcagct gcggaaaatt taaaagcagc gtcggcggca tctcggtttg
      601 ttctttcgaa tcgggatacg ggaagttgat ttttaagaga atgccttttg tgtcagcttc
      661 caaaacagtt tcccgaagct ctgcgcggtc ttttccttta aattcgctct tgattatcgt
      721 tcttatcaat tccgccgcgg ctggtgtgaa aatgtcgccc tgtttggcgc tgctgcgggc
      781 ggctcgaact aatttttgga aagcggcttt atgagcttcg atttgttcgg gcgttgcgtc
      841 tttcggcagt ttcgggagct tttcttcgag ttgctcacga agttttgaat attctttagc
      901 gcttttttca aattcggttg ttgtattttt atctttcggg gagagggtct tctgcgccgt
      961 cgcggtttga tgagcgacga aactacaaag caataaaaat attattgaaa tgtaatattt
     1021 ttttacactt ttcataaaaa attccctcgt aattaagatt tttcatacaa aattcgcctg
     1081 tagtcggaga atttcgcaag aattgtgcca tttggctaaa gctttaacga ttaaatcaaa
     1141 agtgtattta tgcaggaatc aaaaagcgcg cgagcctttc tcaggcgacg gcaaaaaaaa
     1201 ctcgtccgct atgtctattg tcgcacagac aaattaatcc ggatttagta ggatgtattt
     1261 atcaaataaa tattttctcc cctggcggat aagccttcat tcatatccgt tgaaaattgc
     1321 tgtctgaaac aaggcttgac cgggcgtgta aaagctcttg agtttcacag ctttacatct
     1381 cagtgaaaag gtagggcgta tggaaagttt aaatggtatc ttcgtgttgt tctcattctt
     1441 catatttttg tttgcggcgt cgtggctgaa acagaaattc gccaatcgca agaatgataa
     1501 tcactcagac taaaaagcag agccggatat tttgccgtcg gctggtcgaa gtcgaaatca
     1561 ggtacgaagc ttgcaaagta tccgtgcaga gaaattcatt gcattaaatt aatgcaatga
     1621 atttcttttg gcattgaata aattttatct ttttatatat aaaggaattt gcgggcaccc
     1681 acttccggaa actccgaatc ttaattcatt gccctttttt aactttgtat ttataagcgg
     1741 ttggcgattt tatgttgatg caaaaggcta aattcttact ttattacaca gcgttgcgga
     1801 gcgccgtatt tttcgggctt cgccgcacct taaatccttt gaactacaag tggcgttgga
     1861 aatattggaa aaatcgaaag cggtttaaat acgaatatcc gcacatttga gaactttctg
     1921 aaggcgcggg atttgaaacg aaaattgtcg ataattcaat ttcgaatcgg gaaagcatcc
     1981 ggctgcgatt caaggtgttt gtcgccttga atccgacttt cggtcgcctt tacccgtcgt
     2041 cttaattgcg aaaattctgc gtcaccatca agccgcgtct gccgccgtcc ataattccta
     2101 tcccgacgcg tccgaattgc ggtcgcagga tattggcgcg gtgaccgggc gaattcatta
     2161 atccggtgtg cgcgatttga acggtcggag ccagcgcgag attttcgccc gccgttaaaa
     2221 aacgcacctc ggcgcgccgc atacggtcaa acgggtcgag attttccggc gtgttatgag
     2281 aaaaataacc gcgtgcaaac atatcggctg aatgacggcg cgcaacttcg gtcaattccg
     2341 ggtcggcttc gagcggtttc agaccggcgg cgaggcgctc gcgattgacc aattccagca
     2401 tctgcgcttc gatttccggt cgcggtcgcg tttcggaaac cttgaaaggc agctcgaccc
     2461 gctcgtttga ttcgggatga atcgtccgcc ggttcagagt ctgctggacg gcgtcgccga
     2521 aaatagacga aagcgccgtc tccagttctt cggtgtaacc ggcaagataa tttgccgtcg
     2581 cgctctcacg agttttttcc tgtaagccgt cggaaaacgg catcgagaaa agcatcgacg
     2641 acaggattgc cgctaaaatc aaaccgttga caaatcccgg caatgttccg agcaggcgat
     2701 taacgctccg tttatgaata tctcccggca gacggcggag cagcgcgtaa gcgacaattt
     2761 gaaacaatat actgaccgcc gccactatca aaacaaaggc gagcggctga ttccaaacct
     2821 ccgtccagtc gtttatcaaa ccgagccggc gagcgagcgg ctgataaaaa tgcaagcccg
     2881 ccaaaacgct cccgatccat cgcagcaaat cgagcagact taaaataaaa ccgcgatgcc
     2941 agccgtataa aacgctcagt aaaataaaga atatgaggag aacgtcgata aaattgaaac
     3001 tcatatcttt gtattgtaac aattcaaaga aaattttccg tgcgtttaat tactcaaatc
     3061 aataaacgcg aaggaataag aaattcccga ccccgaggaa agaataatta aactacgggt
     3121 gaacgcagat aaagcaggtt tcgcggcttg ataagaaaac ggttttatcc cgtcaatcaa
     3181 gccgcgaaag aaacctgctt cgaatttccc tttaaattat tcgtgtcttc gggctgaaaa
     3241 ttatagcgtt tgctcttccg gcgggaaaaa agctcgcagc cagtcattga gaacgcaatc
     3301 gacaaccaga tgaacgcgat cggacgcgcc gccgtttttg acgcggtgtt tgagattgaa
     3361 attcagatac caagcctcgc cttccttcat ctcgacgctt tgcccgtcga gaaaaaattc
     3421 gacctgtggg ttagtctgga cgggaatgtg caggcgtata aagccgtctt cgtagccgag
     3481 ccggtaatct cgatgctcgc ggatatttgc gcccgcgccg agccgcagaa aacgcgccga
     3541 ttcgagctcg cagcgaaaag ccgccagcac ttccggaata tacgcgcagc gcccgagcat
     3601 ttcggtgtcg gcgtatccgc cggcggcggt cgggtcggga taaagctgat tgaccgccga
     3661 gcctcgcggc gcgcgaagcg caattccgct ccaatcgcct tcgtaataac gagtgttaaa
     3721 atgcggcgtc caatcaatcg ccgcaaagcc tcgcaaatca tttttgagcc gctgcgaatc
     3781 gaaaacaaaa ggtaatttta agactggaat cataatttta tcagggatta atctgcgttt
     3841 agccgtcaaa atctgccgtt tcatttctcg ttatctctgt gcgccttcgg cggctgaatt
     3901 ttctttcgcg gctttcaaaa tcgcggcgag ccgtttcgag tttttcatag agcgggttaa
     3961 cccatctttc agccgccgcg tgtgccgcgc cgctcgcctg ttttttcttg gtttcgctgt
     4021 cggggacaaa attcatccgc ggcgttttgg cgttaaactg cgcggcattt tccatctgtc
     4081 cgatatcttc gggcgcatat tcggcgcgaa aatgtttgag aacggctgag gtgacggctt
     4141 ccggcaattg cgtatagttg acgagcagcg cgtttcgatt cggcagacga ttgagcgcgc
     4201 tttcgcaaat ccgcgccagc acgcgcgcgc agtattcttc cgaaggcatc tgcaaaatgt
     4261 ccggcaaatc cagaccgggc agaatctttt cgaccacgcc tggaatcatc tgcgcgccgc
     4321 gctgcttcat atgcgaagcg ataacttcga ccggattgcg atacagaaaa atccacggga
     4381 cacccggaaa agcgctttct attaaagcca gctcgaacgc gctccaactg tcgaatttaa
     4441 taaaataata tttctcttcg gcgtttcttt tttgaccgaa tgcgccgata atccatcgca
     4501 gccactggat tctttgttca tcggaaattt ccggattttt aaaattcgag cgcaaaaccg
     4561 aatcgaccgg cggcggctcg gacacgacga tattttgcgc cgacgcggcg agcatctgcg
     4621 caatcagcgt cgagccgcac cgcgacatat ggaagataaa accggtcggc gcaatcccgc
     4681 tcgatgccgc gtgtaattcg cccagaattt ctatcggcgt ctggtgacga aacaataaat
     4741 taaaaggctc gctaaagcgc cgctcgatcg tctggtcaaa aaaaggttcg gtaaaacgct
     4801 tttttcccag ataacaccaa tcgaccagcg ggcgattttc ctgccagtag acgcgcgccg
     4861 gcatccaatc gcgcaaattt tcgagcgttt gattcatatc catctttcca gccaggcgcg
     4921 gcgattgcgg cgcatcgctt cctcgacttc ttccggcgta attttatagc cgcgttccgc
     4981 gctgatttcg actatttttt caataaaaac aggtcggtcc gtaatcttgc gcagtcgctc
     5041 ctgaagcgac aaat
//
LOCUS       DASWNE010000310         1806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_150967, whole genome shotgun sequence.
ACCESSION   DASWNE010000310 DASWNE010000000
VERSION     DASWNE010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150967"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1806)
                     /locus_tag="VGB68_06395"
     CDS             complement(<1..>1806)
                     /locus_tag="VGB68_06395"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0048893.1"
                     /translation="RPNIFTQSVANILPGEKIIIEISYVETLKYEDGQYEFVFPMVVG
                     PRYIPGNPTGQQGGGFSPDTDKVPDASKITPNVAKERAGHDISIEVKLDAGVPLETVA
                     SQSHQIESNRLAAGSSLIRLKNEKTIPNKDFILLYDVAGKKIEDAVLMHRAEKGGYFT
                     LILQPPDNVRPQDATPKEIVFVLDTSGSMNGFPIEKAKESMKLALDGLNPQDTFNLIT
                     FAGDTHVLFDKPVPATTENMQKAQAFLASRQGGGGTEMMKAIKTALEPSDSQQHLRIV
                     CFMTDGYVGNDKEIIAEIQKHQNARVFSFGIGSSVNRFLLDKMAEEGRGEVEYVALTD
                     DGSAAARRFHERVRSPLLTDISLDFGNLQVADVYPKRINDLFSAKPVVVHGRFTRAGS
                     GTIRLKGKSFGREVVREIPVNLPENEPNHDVLATLWARERIDDLTSQDYTNQKPEIKE
                     TITNLGLEYRLLTQFTSFVAVEERIVTDGGQPRRIEVPVELPDGVSREGVFGDLKSDR
                     LETQAVRLNKSQYLRPVPRKANDARNIAPTLPARKVKREVSKGYKDWSDKDVAYIVSG
                     EEKQAVGEKTASEIIKATSLPKPEYPSAARAVKAAG"
BASE COUNT          365 a          496 c          468 g          477 t
ORIGIN      
        1 gcccgccgct tttacggctc tggcggcgct cgggtattcg ggtttcggca ggctcgtcgc
       61 tttgataatt tccgaagcgg ttttttcgcc gaccgcctgt ttttcctcgc cgctgactat
      121 gtatgcgacg tctttatccg accagtcttt gtaacctttg gaaacttctc tctttacctt
      181 tctggcggga agtgtaggcg cgatgtttct cgcgtcattc gcctttcttg gaacaggtcg
      241 taaatattgt gatttattca gacgaacagc ctgagtctcc agcctgtcag actttaaatc
      301 accgaaaacg ccttcgcggc tgacgccatc gggcaattcg accggaactt cgatgcggcg
      361 cggctgtccg ccgtcggtaa cgattctttc ctcgaccgcg acaaacgacg taaactgcgt
      421 caacagacga tattcaagcc ccagattcgt aatcgtttct ttaatttcgg gtttctggtt
      481 cgtgtaatcc tgcgacgtta aatcgtcaat cctttcccta gcccagagcg tcgccagcac
      541 gtcgtgattc ggctcgtttt cgggaagatt gaccggaatt tccctgacga cttcgcgacc
      601 gaaagatttc cctttcaggc gaatcgtgcc gcttcccgct ctggtaaaac gcccgtgaac
      661 gacgacgggt ttcgcgctga aaaggtcgtt gatgcgtttc ggataaacgt cggcgacctg
      721 cagatttccg aaatcgagcg agatgtccgt caaaagcggg ctgcgcacgc gctcgtgaaa
      781 acgccgcgcg gcggctgagc cgtcgtccgt tagcgccacg tattcgacct cgccgcgacc
      841 ttcttccgcc attttgtcga gcaggaaacg attgaccgag ctgccgatgc cgaacgaaaa
      901 cacgcgcgca ttctggtgtt tctgaatttc cgcgatgatt tccttatcgt tgccgacata
      961 accgtcggtc ataaaacaaa caatgcgaag gtgctgctgc gaatccgaag gctcaagcgc
     1021 ggttttgatc gccttcatca tttccgtccc gccgccgccc tgccgcgagg cgagaaacgc
     1081 ctgcgctttc tgcatatttt ccgtcgtcgc cggaacgggt ttgtcgaata aaacgtgcgt
     1141 gtcgccggca aaagttatca aattaaaagt gtcctgcgga ttcagcccgt cgagcgcgag
     1201 cttcatcgat tctttcgctt tttcaatcgg aaatccgttc atcgaaccgc ttgtgtcgag
     1261 cacgaaaacg atttctttcg gcgtcgcgtc ctgcggtcgg acgttatcgg gcggctgcaa
     1321 aatcaaagta aaatatccgc ctttttccgc gcgatgcatc aaaacggcgt cctcgatttt
     1381 tttgccggcg acgtcgtaaa gcaaaataaa atctttgttc ggaatcgttt tttcgttttt
     1441 cagcctgata agagagctgc cggcggctag ccggttcgat tcgatttggt gcgattgcga
     1501 agcgaccgtt tccaacggaa ctccggcatc caatttaact tcaatcgaaa tatcgtgacc
     1561 ggcgcgctct ttcgccacgt tcggcgtaat cttcgaggcg tcgggaactt tatcggtgtc
     1621 gggcgaaaaa ccgccgccct gctgtccggt cggattgccc ggaatatagc gcggtccgac
     1681 gaccatcgga aagacgaatt cgtattgccc gtcttcgtat ttcagggttt cgacgtagct
     1741 gatttcgata ataatttttt cgccgggcag aatgttggcg accgattgag tgaagatatt
     1801 cgggcg
//
LOCUS       DASWNE010000311         1816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_44946, whole genome shotgun sequence.
ACCESSION   DASWNE010000311 DASWNE010000000
VERSION     DASWNE010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44946"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /gene="gcvH"
                     /locus_tag="VGB68_06400"
     CDS             <1..358
                     /gene="gcvH"
                     /locus_tag="VGB68_06400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEY0048894.1"
                     /translation="KDHEWVSVDGDNATIGITDYAQHQLGDVVYVELPKAGETFTEHE
                     AFGSVESVKAVSEIFTPISGEVTEVNDGLNDTPEIVNSDPYEGGWMVKLKMSNAGEID
                     ALLSAAEYEEYLNATA"
     gene            460..1806
                     /gene="gcvPA"
                     /locus_tag="VGB68_06405"
     CDS             460..1806
                     /gene="gcvPA"
                     /locus_tag="VGB68_06405"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEY0048895.1"
                     /translation="MRYIPNSPEEREEMLGVVGLNSAEELFRSIPNEVKLNRELNITE
                     PLAEPEVIGAMENLANRNTASRKTSFLGAGVYSHFSPTIVDHLIQRSEFFTSYTPYQP
                     EISQGTLQSIFEFQTLVCQLTGMDVANASMYDGSTSMAEAFLMAQRVTRRDKVVIAET
                     VHPEYLAVAKTYTQHGDLTMETVHFDKETGRVLADDLNKLDDKTAALVIQSPNFFGCI
                     EDLQALADKAHAVGALLVVVVTEAISFGLLKSPGACGADIVVAEGQSFGIPMSFGGPH
                     VGLFACKDKYVRQMPGRLAGIAYDKNGNRGFVLTLATREQHIRRDKATSNICTNQGLI
                     ALAATIYMEAMGKKGLQEVAFQNAQKAAYAARKIAEIEGFSLAFSAPKFNEFVVRAPK
                     DAGGILESLRAGKDIIGGLALSRYYAENPNDFLVCVTETNTKAQIDALVEELGKLR"
BASE COUNT          437 a          489 c          525 g          365 t
ORIGIN      
        1 caaagaccac gaatgggttt cggttgacgg cgacaacgcg acaatcggaa tcacggatta
       61 cgcgcagcac cagctcggcg acgtcgttta cgtcgaactg ccgaaggcgg gcgagacctt
      121 caccgagcac gaggcattcg gctcggtcga atcggtcaaa gccgtttccg aaatctttac
      181 gcccatcagc ggcgaagtca ccgaagtcaa cgacggtttg aacgatacgc ctgaaatcgt
      241 caattcagac ccgtacgaag gcggctggat ggtcaagctg aaaatgagca acgcgggcga
      301 aatcgacgcg cttttgtccg ccgccgaata cgaagaatat ctgaacgcta ccgcgtaaaa
      361 tcggttttcg gtcttctgtc ttcggtcttt gaatcaggca gcgacttgag cttcatcgac
      421 acgaagaccg aagaccgaag accaaagacc aaagattaaa tgagatacat cccaaattca
      481 cctgaagagc gcgaagagat gctgggcgtc gtcggcttaa attcggcgga agagcttttc
      541 cgctcgattc cgaacgaagt caagctcaat cgcgagttga acatcaccga gccgctcgcc
      601 gagccggaag ttatcggcgc gatggaaaat ctggcaaacc gaaatacggc ttcgcgcaaa
      661 acttcgtttt taggcgcggg cgtttattcg catttttcgc cgacgattgt cgaccacctg
      721 atacaacgct cggagttttt tacgtcctac acgccgtacc agccggaaat ttcgcaaggc
      781 acgctgcaat ctattttcga gtttcagacg ctcgtctgcc agctgaccgg aatggacgtg
      841 gcaaacgctt cgatgtatga cggctcgacc tcgatggcgg aagcgtttct gatggctcag
      901 cgcgtcacgc ggcgcgataa ggtcgtcatt gccgaaaccg tccatcccga atacctggcg
      961 gtggcgaaaa cctatacgca gcacggcgac ttgacgatgg aaaccgttca tttcgacaag
     1021 gaaaccgggc gcgtgctggc ggacgattta aacaagctgg acgataaaac cgccgcgctg
     1081 gtcattcaat cgccgaattt cttcggctgt atcgaagatt tgcaggcgct ggctgacaaa
     1141 gcgcacgccg tcggcgcgct tttagtggtc gtcgtgacgg aagcgatttc cttcggcttg
     1201 ctgaaatcgc cgggcgcgtg cggcgcggac atcgtcgtcg ccgaaggtca aagcttcggg
     1261 attccgatga gcttcggcgg accgcacgtc gggcttttcg cctgcaaaga caaatacgtg
     1321 cgccagatgc cgggcaggct cgccggaatc gcttacgaca aaaacggcaa tcgcggtttc
     1381 gttttgacgc tggcgacgcg cgagcagcac atccggcgcg acaaggcgac ctcgaacatc
     1441 tgcaccaatc agggcttgat tgcgctcgcc gccacgattt atatggaagc gatgggcaaa
     1501 aaaggcttgc aggaagtcgc ctttcagaac gcgcagaaag cggcttacgc ggcgcgaaag
     1561 attgcggaaa tcgaaggctt ttcgctggcg ttcagcgcgc cgaaatttaa cgagttcgtc
     1621 gtccgcgcgc cgaaagacgc gggcgggatt ctggaaagcc tgcgcgccgg aaaagatatt
     1681 atcggcgggc ttgccttgtc gcgttattac gcggaaaacc cgaacgattt tctcgtctgc
     1741 gtgacggaaa cgaacacgaa agcgcagatt gacgcgctgg tcgaagagct tggtaaattg
     1801 agatgaaccg gatgtt
//
LOCUS       DASWNE010000312         1687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_151038, whole genome shotgun sequence.
ACCESSION   DASWNE010000312 DASWNE010000000
VERSION     DASWNE010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151038"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..802)
                     /locus_tag="VGB68_06410"
     CDS             complement(8..802)
                     /locus_tag="VGB68_06410"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HEY0048896.1"
                     /translation="MRKVRFLLAGCLLLCGGLVAEAQKPEKPAPGNQNNAATEREIRE
                     FFNSYAEDLRRHRGEAIAERYDSRGYFRMGNGAKTLVSFEDNKKRYLSDWAGPKSFEW
                     KDLSIEVLSKDSAVVTALFDWQVGAGEARTYSYTGLLIKQSGKWRIRVEDESGAALTG
                     TTPITGSSSAAGAFKYLYKALAGASIAAHRHSVDMRITVRSGRKFILMGDLAKATVQI
                     FEAGSTFTIPAGTWHVEWWETDTVEEIEGTGPMLTERATPKTPRMP"
     gene            complement(925..>1687)
                     /locus_tag="VGB68_06415"
     CDS             complement(925..>1687)
                     /locus_tag="VGB68_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048897.1"
                     /translation="RTMKIEELAYHKTPLGELTLRRRTETLLQNREVFEVKLGDEFLM
                     SSLFTEGEKQLSHLGLAGLGGELDVVVGGLGLGYTAAAALENKMVRSLLVIDLFQEVI
                     DWHQNKLVPMGSVLSEDKRCEMRQGNFFELAQTGFDVSDNKRKFDAVLVDIDHSPGHF
                     LDEKNKSFYAAAGLESLREQIKTGGAFALWSNEACDEKFTKHLESIFGTATAQTIEFA
                     NPYTNSISVNSVYVAHKKSIPKFSMISRETENPMI"
BASE COUNT          366 a          473 c          396 g          452 t
ORIGIN      
        1 aagcctttta aggcatacgc ggcgtcttcg gggttgcccg ttccgtaagc atcggaccag
       61 tgccttcgat ttcctcgacc gtgtctgttt cccaccattc gacgtgccat gtgccggcag
      121 ggattgtaaa ggtcgagcct gcctcaaaaa tctgcaccgt agccttagcc agatcgccca
      181 tcagaataaa tttacgcccg ctgcgaaccg taatgcgcat atcgaccgag tggcgatgag
      241 ccgcgatgct cgcccccgcc aaagccttgt agagatattt gaatgctcca gccgccgagc
      301 tgctgcccgt gatgggagtc gttccggtga gcgccgcgcc ggactcgtcc tcgacgcgaa
      361 ttcgccattt gcccgattgt tttatcaata atccggtgta tgaataagtt cgcgcctcgc
      421 cggcgccgac ctgccagtca aaaagcgcgg tcacaacggc ggaatctttt gacaaaactt
      481 caatcgaaag gtctttccac tcaaaagatt tcggtcccgc ccagtcggac agataacgtt
      541 ttttgttatc ttcgaatgaa accagcgtct tggcgccgtt gcccattcga aaatagccgc
      601 gcgaatcgta acgctcggct attgcctcgc cccggtgacg gcgcaaatcc tcggcgtagg
      661 aattaaaaaa ctctcttatt tcgcgctccg tcgccgcatt gttttgattt cccggtgccg
      721 gtttttcggg tttctgcgct tcggcgacaa gaccgccgca cagcaacagg cagcctgcca
      781 atagaaatcg tacttttctc ataatgcacc tttaagctct ttatcgagcc tttcagcttc
      841 tgtcctgtgc cggattaaat tcggcataaa atcttatcac gctttgaccc tttccgccgt
      901 cccgccggtc gctgccgttt cggctcatat catcggattc tccgtttccc ggcttatcat
      961 actgaatttc ggaattgatt ttttatgggc gacatagacg gagttaacgg atatggaatt
     1021 cgtgtaagga tttgcaaatt cgattgtctg agccgttgcc gtgccgaata tggattctaa
     1081 atgttttgta aatttctcgt cacacgcttc atttgaccag agggcaaacg cgccgcccgt
     1141 ctttatttgt tcgcggaggg attcgagtcc cgcggcggcg taaaaggatt tgttcttttc
     1201 gtcgagaaaa tgtccgggcg aatggtctat atcgaccaga accgcatcga attttctttt
     1261 attatcggaa acgtcaaaac ccgtctgtgc cagctcgaaa aagttgcctt gtcgcatctc
     1321 gcatctttta tcttccgata atacgcttcc catcggaaca agtttgtttt gatgccagtc
     1381 gatgacttcc tgaaacaaat caataaccag aagcgaacgc accattttat tttccagtgc
     1441 cgccgctgcc gtataaccca gaccgagccc gccgacaacg acgtcgagtt cgccgccgag
     1501 ccccgccaag ccgagatgcg agagctgttt ttctccctcg gtaaaaagac tcgacatcaa
     1561 aaactcgtcg ccgagcttta cttcgaaaac ctctctgttt tgcaaaagag tttccgttct
     1621 ccggcgcagc gtgagctcgc ccaaaggcgt tttgtgatag gcgagttctt cgattttcat
     1681 cgttctt
//
LOCUS       DASWNE010000313         2367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_679969, whole genome shotgun sequence.
ACCESSION   DASWNE010000313 DASWNE010000000
VERSION     DASWNE010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679969"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..463
                     /locus_tag="VGB68_06420"
     CDS             68..463
                     /locus_tag="VGB68_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048898.1"
                     /translation="MKHSKKLLTLIFATVLFTGLAVADASAQRRGGRFIRRPVIVRQY
                     YVQRYNPYWYWNSWDSPYFYDPYLSERRQRYYLERELRGNREELRKHLEKYNADGVIT
                     AKERKELDDDYRDVARSQRNLNQFRRRYY"
     gene            508..747
                     /locus_tag="VGB68_06425"
     CDS             508..747
                     /locus_tag="VGB68_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048899.1"
                     /translation="MPRFFLSIAGAIKKTSRLIGHGLGSAIPIAPFEDLEKLKTLPAG
                     IVVLRTMNIRKDYPESRIRRQTCEPNALQEITKQQ"
     gene            830..1210
                     /locus_tag="VGB68_06430"
     CDS             830..1210
                     /locus_tag="VGB68_06430"
                     /inference="COORDINATES: protein motif:HMM:NF012261.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004869 - cysteine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystatin domain-containing protein"
                     /protein_id="HEY0048900.1"
                     /translation="MKKYFQTALALGVIFSFAAIAFAQVKPRVGGYKEISKTSAEVRA
                     AAEYALEAQGEKDDAFYRLKAVEKAESQTVAGTNYRLCLQVIIEDEETGEEIQEFYRA
                     IVFRNLKKEFTLKSWTESDCGEKE"
     gene            1283..1705
                     /locus_tag="VGB68_06435"
     CDS             1283..1705
                     /locus_tag="VGB68_06435"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0048901.1"
                     /translation="MTYQITCEEDAEPEDIQILSDGINNYSAEKVTGGARRDLTFFLR
                     DPEGSIVGGVHGNSGWGWLYISDLWVSETIRGGGYGTALMREAEREATNRGCRGIYLD
                     TFSFQAVGFYLKLGYEIFGELEDFPPGYKRVFLRKILS"
     gene            2010..>2367
                     /locus_tag="VGB68_06440"
     CDS             2010..>2367
                     /locus_tag="VGB68_06440"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0048902.1"
                     /translation="MKKIFIVAVLIAASVSAAFSQNQPDKPFGKTAADAQKLIRYMTD
                     WANLSNKHDIAALDKMLPADLVITFQDGEVLTKDEYIAALKKNPGDFTIKYDDQKVDL
                     FNDKTAVFRARYVVSVG"
BASE COUNT          672 a          590 c          562 g          543 t
ORIGIN      
        1 aaggacaggc gtaaattatc tcttaccgag caattgcctg ttcgttctta aagaccaggg
       61 aggttaaatg aaacactcaa aaaaattact tacattgatt tttgcaacgg ttctttttac
      121 aggtttagcg gtggcggatg cttcggcgca gcgtcgcggc ggtcggttta ttcgacgccc
      181 cgtcatcgtc agacaatact atgtccagcg gtataatccg tattggtatt ggaattcctg
      241 ggacagtcct tatttctatg acccttattt gagcgagcgg cgacagagat attatctcga
      301 aagagagctg cgcggcaatc gggaagaatt aagaaagcac ctggaaaaat acaatgccga
      361 cggcgtgata accgccaaag agcgaaagga attggacgac gactatcggg acgtggcgcg
      421 ctcacaacgg aatttgaatc aattcagacg ccgttattac taaagaagtc cgataacttt
      481 caatcctatt ttcaattaag cgaggtaatg cctcgctttt ttttgtcaat cgccggagca
      541 attaaaaaaa catctcgcct aatcggacac ggactcggtt cagccatccc gattgcgccg
      601 tttgaagacc tcgaaaaact caagactctg cccgccggaa tcgtcgtgct gcgaacgatg
      661 aatatccgca aagattatcc ggaaagccga atccggcggc aaacctgcga gccgaacgcg
      721 cttcaggaaa taactaaaca gcaataaata caatcacttg acttgcccgc cgaaagagcg
      781 taaaactgag cttcgaagat agaccaatca aaactattcg aggttccaaa tgaaaaaata
      841 ttttcagacc gctctggcgc tcggcgtaat tttcagcttt gccgccatcg ctttcgcgca
      901 ggtcaaaccg cgcgtcggcg gttataagga aatttccaaa acttccgccg aagtccgcgc
      961 ggcggctgaa tacgccctcg aagcgcaggg agagaaagac gacgctttct accggctcaa
     1021 agcggtcgaa aaagccgaaa gccagacggt cgccggaacc aattaccgcc tgtgcctgca
     1081 agtaattatc gaggacgaag aaacgggcga ggaaattcag gaattttacc gggcgattgt
     1141 tttcagaaat ctgaaaaaag agtttacgct caaaagctgg actgaatcgg actgcggcga
     1201 aaaagaataa tatatcagct aaaacattta acccggcgcg cttcgataaa agcgcgggac
     1261 ggcgccggcg caaattaccg ttatgactta tcaaatcacc tgcgaagagg acgccgagcc
     1321 cgaagacata caaattttaa gcgacggcat taataattat tccgccgaaa aagtaacggg
     1381 cggcgcgcgc cgtgatttga ccttttttct tcgcgacccc gaaggctcga ttgtcggcgg
     1441 cgttcacggc aacagcggct ggggctggct gtatatcagc gacctctggg tttccgaaac
     1501 aatcagaggc ggcggttacg gcacggcgct gatgcgcgaa gccgagcgcg aagcgacgaa
     1561 tcgcggctgt cgcggcattt atctcgacac tttcagcttt caggcggtcg gattttatct
     1621 aaaactcggt tacgagattt tcggcgagct ggaagatttc ccgcccggtt ataagcgagt
     1681 cttcctgcga aaaatattga gctgaacacg cttggcgcaa ttaagaatta cgaattggaa
     1741 gacataattt atcattcgca attcgtaatt cgcaatcaac aaaagtcgcc cttagtaatt
     1801 taataaattt cttgacttgc cctctcgaac aaagtaaatt gaggcaaatt tatttcggag
     1861 cgtttccggc ggatttcgcc cgcggatatg aagtccggat tcactttcag ctcgtatcgc
     1921 ttgacgaatt ccggcggcag tttcagcccg cctcttgtaa atcgatataa aatttccgca
     1981 ttaaaaaaat ccgaatgcct gataaaacta tgaaaaaaat attcatcgtc gccgttttaa
     2041 tcgccgcatc cgtttccgct gctttcagcc agaatcagcc ggataaacct ttcggcaaaa
     2101 ccgccgccga cgcgcaaaag ctgattcgat atatgacgga ctgggcaaat ctctccaaca
     2161 aacacgacat cgccgcgctc gataaaatgc tgcccgccga cctcgttata acttttcagg
     2221 acggcgaggt tctcaccaaa gacgaataca tcgccgctct taaaaaaaat ccgggtgatt
     2281 tcaccatcaa gtatgacgac cagaaagtcg atttattcaa cgacaaaacc gccgttttcc
     2341 gcgcccgcta cgtcgtcagc gtcggag
//
LOCUS       DASWNE010000314         8665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_151119, whole genome shotgun sequence.
ACCESSION   DASWNE010000314 DASWNE010000000
VERSION     DASWNE010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8665
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151119"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VGB68_06445"
     CDS             complement(<1..306)
                     /locus_tag="VGB68_06445"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: protein motif:HMM:NF016438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEY0048903.1"
                     /translation="MRRESKAVKIRDVQVGGGAPVAVQSMTKTDTTDVDGTIKQIEEM
                     VAAGCEIVRIAVPDKDAAIALKEIRKRTSVPLVADIHFHYKLALMALEAGIDKLRINP
                     "
     gene            complement(430..1791)
                     /gene="rseP"
                     /locus_tag="VGB68_06450"
     CDS             complement(430..1791)
                     /gene="rseP"
                     /locus_tag="VGB68_06450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEY0048904.1"
                     /translation="MEIIIAILAVVFVLGVAINIHEFGHFIVAKLLGMRVEAYSFFGL
                     GPRIWGFKRGHTDYRISAIPLGAYVKLYGDEGTSALEGGESKGEAVPESELYELRPRW
                     QKFLVMVGGPMMNILLALAIPFSMALVKGVPAPPSPIVEYVKPGGAGDKAGIKPGDRI
                     VVFEGTENPTWSRIQNDSMLSPEKEIPVVVERNGQRLPLTVTPSKISESGNNIGLLDI
                     EPEGETQPVVAERVVEGSPAEQAGLQKGDLITSFNGAPVRNPADAKQKVAENGTNPIR
                     MMVNRNGEAKELVVTAKVEQDNVPRVGVMFGSQYSKSRESVGVVGAAAYAWNSNMEIL
                     RMTAKAFGQVSKGERSAGETFSGPIGIVQIISQVAATAGFEGLLFVLGLISLNLGIFN
                     LLPIPMLDGGQIMVLGIEKVYSWFGRTLSMAVKERIQLVGLAVILCLMVFTIFNDISR
                     FFR"
     gene            complement(1891..3081)
                     /locus_tag="VGB68_06455"
     CDS             complement(1891..3081)
                     /locus_tag="VGB68_06455"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:NF009114.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEY0048905.1"
                     /translation="MKAVSILGSTGSIGCNTLKVVQHLGNFRVVALGAGRNMQLFAEQ
                     IARFEPELVSCENESCAEKLLENLHALGAPKIPKIELGEKGLIAVATHEAAETVVSAT
                     VGAVGFVPTLRAIEAGKRIALANKETLVMAGELMTKAARENKAAILPVDSEHNALHQC
                     LRGESKAEVKRLILTASGGPFRTKPKEEIENATVEEALNHPNWKMGDKITIDCATLMN
                     KGLEVIEARWLFDFDADEIAVVVHPQSIVHSMVEMVDGSVIAQLGVTDMKHAIQYALT
                     YPERQPGCLEPLDFRKISQLTFEEPDFERFPCLALAYKALKTGGTMPAVLNAANEIAV
                     QAFLDGRIRLSEIPQVNASVMNEHRAQAASSLEIVLQADNQARKQAGEVVAAKSAAAR
                     GSAN"
     gene            3262..3552
                     /locus_tag="VGB68_06460"
     CDS             3262..3552
                     /locus_tag="VGB68_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048906.1"
                     /translation="MKKLLTISALLGSLIIFAPSQEAKAASAASIAEPQIRIQLGQDR
                     RWRNRRSTIQTRVVRRGFATYRETYRVTYRRNGSVRTELISRVRIGGRRYSY"
     gene            complement(3592..4182)
                     /locus_tag="VGB68_06465"
     CDS             complement(3592..4182)
                     /locus_tag="VGB68_06465"
                     /inference="COORDINATES: protein motif:HMM:NF019241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HEY0048907.1"
                     /translation="MSEQILQNVLDEAIAAFRGQKKLAEGAIEQVSDDEFFRLIDAEA
                     NSIAALVKHLGGNLRSRWTRFLTTDGEKADRHRDSEFVTENDTRESLMQAWESGWRAL
                     FQALESLRTDDLGKIVKIRTEDFTVVKAIYRALSHSGQHVGQITLLAKHFRSSDWKTL
                     SIPKNKSEEFRQFMANLEDKGNYLETAREFSGKEAK"
     gene            4494..5417
                     /locus_tag="VGB68_06470"
     CDS             4494..5417
                     /locus_tag="VGB68_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048908.1"
                     /translation="MNQSTGQSRLVSGLFNDRQSAERVYSSLESRGYSKDDVNLMMSD
                     ETRNRHFGEGTPETELGSKALEGAGAGSAIGGTLGAIIGGIAAIGTNVLLPGLGLVVA
                     GPIFAALAGAGAGGLTGGLVGALIGSGIPEEHAAEYESGIKNGGVYMGVNARNDEDAQ
                     YFHEEFNRHGGSHVVGTGLGVAGGAATGAAIGTAIAPGVGTVGGGIVGAVAGGIAGGA
                     AGHEIAEKVNPQPGDEHDGDHNVGTGTGAVGGAVTGAAIGSVVPGVGTAAGGVVGGVV
                     GGVGGAVAGHEVAEKVNPDDKLIDDDNVTRR"
     gene            complement(5600..6928)
                     /gene="hisS"
                     /locus_tag="VGB68_06475"
     CDS             complement(5600..6928)
                     /gene="hisS"
                     /locus_tag="VGB68_06475"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEY0048909.1"
                     /translation="MSNTNPARGMRDFLPVDVRRREYVIGIIKQVYESYGFEPLETPA
                     VENLETLMGKYGEEGNQLIFKILKRGEKLNADDKELSDLALRYDLTVPLARVVANHKN
                     DLPKFFKRYQIQPVWRADRPARGRFREFFQCDVDAIGSNSMIIEAEIISAASEILQRL
                     GFNDFVVRLNHRDILRGILEAVQIEADKQSDALIALDKLDKIGKDGVLKEFVQRDIDE
                     SAGLRLLDLFAAVETAGNTDKLSRLAEFVGEQETGKNGLNELAQILGLAGLENIEIDA
                     SLARGLSYYTGAIMEIVLTGGDFAGSIGGGGRYDELIGMFGKEQIPACGFSLGLERII
                     VVMTERNMFPEDLQNAPADVLVTIWNDDSVGESLKLANDLRSKGLRVLVYPEADKLGK
                     QFKYASQINAPFVCVLGETELAENKVTLKNMQSGAQETLLREDIAKKIQA"
     gene            complement(6934..>8665)
                     /locus_tag="VGB68_06480"
     CDS             complement(6934..>8665)
                     /locus_tag="VGB68_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HEY0048910.1"
                     /translation="SDENSESQASMEKITITGDALTLGDVVKVACQNVQVELGNLEKV
                     RKSRDFIARKVAENAIIYGVTTGFGAKADQIIAPEDAKQLQINLLRSHATGVGKNFPT
                     RVVRAIMLIRLNTLVKGYSGVKVDTLERLRDFLNFQIHPLVPEQGSLGASGDLCPLSH
                     LALPLIGEGYVEYKGAEYKTAEFLERLKADEFSGLKEAKDNLLKPFELSYKEGLALNN
                     GTTVMAALGTFAVYEFERLLKLATLVSSLMLEAFCARRNAFDEKIHRVRHHAEQETVA
                     GWIRKFTENSSFIGISSEALRAKSKTAGIEVEETDWLERKKDFAERLSDLKGRTQDSY
                     SIRCMPQVFGASLQVLNHAKGIFANELNAAVDNPLIFVEEDVVLSGGNFHGQPLAFAL
                     DYMKLAISEVGNLVERQINKLHDKATNDLLEPFLVYDEKNINSGLMITQYVAASLVSE
                     NKVLVHPASADSIPTSANTEDHVSMGATAGRQALQILENVKNVVVIAVLTAHHAAEIR
                     RNQFEKLGLETGMGKATGELFEEIKNAVPDYREKDFLNNDRFLYDDIKRLADNYENLA
                     AVAERNLEEQ"
BASE COUNT         2037 a         2316 c         2148 g         2164 t
ORIGIN      
        1 cggattgatt ctgagcttgt caatcccggc ttcgagcgcc atcaaagcca gtttgtaatg
       61 aaaatgaatg tcagcgacca gcggaacgct tgtccgcttg cgaatttctt ttaaggcgat
      121 agccgcgtcc ttgtcgggaa cggcgatgcg aacgatttcg cagcccgccg cgaccatttc
      181 ctcgatttgt tttatcgtcc cgtcaacgtc agtcgtatcg gttttcgtca tcgactgtac
      241 cgcgaccggc gcgccgccgc cgacctggac gtctctgatt ttgaccgctt ttgattctct
      301 tctcataaaa tttcctgcca gccgaaaata ttatagcgcc tgcgaagtaa aaaccaactt
      361 cacagacgct acaaacaaat tacattactt taaaactgcg cttttggctt gcgccgcggc
      421 ttctatcgtt tagcggaaaa accgcgaaat atcgttgaaa atcgtaaaga ccatcaaaca
      481 cagaatcacg gctaaaccga ccagttgaat gcgttccttg accgccatcg aaagcgttct
      541 gccgaaccag ctgtaaactt tttcgatgcc gagaaccata atctgaccgc cgtcgagcat
      601 cggaatcggc aaaagattaa agatgccgag attcaggctg atcaagccca ggacaaacaa
      661 taaaccttca aatcctgcgg tggcggcgac ctgcgaaata atctggacga tgccgatcgg
      721 acccgaaaaa gtctcgcccg ccgagcgctc gcctttcgag acctgcccga aggctttcgc
      781 cgtcatccgc agaatttcca tatttgaatt ccacgcgtag gcggcggctc cgacgacgcc
      841 gacgctttct ctggattttg aatattggct gccgaacatt acgccgacgc gcggaacgtt
      901 gtcctgttcg accttggcgg taacgaccag ctctttcgct tcgccgttgc ggttaaccat
      961 catccgaatc gggttcgtcc cgttttcggc gaccttttgt ttggcgtcag ccggatttct
     1021 gaccggcgcg ccgttaaagg aagttatcaa atcgcctttt tgcagtccgg cttgttccgc
     1081 cggcgagcct tcgacgactc tctcggcgac gaccggctgc gtttcgcctt ccggctcaat
     1141 atcgagcaat ccgatattat tgccgctttc cgagatttta ctcggcgtca cggtcaaagg
     1201 aaggcgctga ccgtttcttt cgacgacgac cggaatttcc ttttcgggcg aaagcatcga
     1261 atcgttctgg attcgactcc acgtcggatt ttcggtgcct tcaaagacga cgattcggtc
     1321 gccgggtttg attccggctt tatcgcccgc gccgccgggc ttgacgtatt cgacaatcgg
     1381 cgaaggcggc gccggaacgc ctttgaccaa agccatcgaa aacggaatcg ccagcgccag
     1441 caaaatattc atcatcggtc cgccgaccat caccagaaat ttctgccagc gcggtcgcaa
     1501 ttcgtaaagc tccgattcgg gcacggcttc gcccttcgat tcgccgcctt cgagcgcaga
     1561 ggttccctcg tcgccgtaaa gcttaacgta agcgccgagc ggaatcgcgc tgatgcgata
     1621 atcggtatga ccgcgtttaa agccccagat tctcggtccc agaccgaaaa acgaataggc
     1681 ttcgacgcgc attcccagaa gcttggcaac gataaaatgc ccgaattcgt ggatattgat
     1741 tgccacgccg agaacaaaaa cgacggctaa aattgcgatg attatttcca tattagaatc
     1801 tttccgagtg caaagaagta aagcttagaa tcgctttcgc cgttctaagt tgcaaataaa
     1861 gtgtaagact cgcccttcgc agagtcaaat ttaattagcc gaaccgcggg cggcggcgga
     1921 tttcgcggcg acgacttcgc cggcttgctt tcgtgcctga ttatcggctt gcaaaacaat
     1981 ttccagcgag ctcgccgctt gcgctcgatg ctcgttcatc accgacgcgt tcacctgcgg
     2041 gatttcggaa agacgaatcc tgccgtctaa aaacgcctgc acggcgattt cattggcggc
     2101 gtttaaaacg gctggcattg tgccgccggt tttcaaagct ttgtaagcca atgccagaca
     2161 cggaaaacgc tcaaaatccg gttcttcaaa agtgagctgc gaaatttttc ggaaatcgag
     2221 cggttccaga cagcccggct gacgctcggg ataagtcaga gcgtattgaa tagcgtgttt
     2281 catatcggtc acgccgagtt gagcgataac ggagccgtcc accatctcga ccatcgaatg
     2341 gacgattgac tgcggatgca cgacaaccgc aatctcgtcc gcatcaaaat cgaaaagcca
     2401 gcgcgcttcg ataacttcca agcctttgtt catcagcgtc gcgcaatcaa tcgtgatttt
     2461 gtcgcccatt ttccagttcg gatgatttaa agcttcttcg accgtagcgt tttcgatttc
     2521 ttctttgggt ttcgtccgaa aaggtccgcc cgaagcggtt aaaataaggc gtttgacttc
     2581 cgctttcgat tcgccgcgca ggcattgatg aagcgcattg tgttcggaat cgaccggcaa
     2641 aatcgccgct ttattttcgc gcgcggcttt ggtcatcaat tcgcccgcca tcacgagcgt
     2701 ttccttattt gccagcgcga tgcgttttcc ggcttcgatg gcgcgcaagg tcggcacaaa
     2761 accgacggcg ccgaccgtcg ccgaaacgac cgtctccgcc gcttcgtgcg tggcgacggc
     2821 gattaagcct ttttcgccca attcgatttt cgggattttg ggcgcgccga gcgcgtgcag
     2881 attttccagc agcttttcgg cgcacgattc gttttcgcag gaaaccagct caggctcgaa
     2941 gcgggcgatt tgctcggcga aaagctgcat attgcgtccc gcgccgagcg cgaccacgcg
     3001 aaaatttccc agatgctgga cgacttttaa agtgttgcag ccgattgaac ccgttgaacc
     3061 taaaattgag acggctttca taaaactaga attcagatta ccacaaacaa aaatttaaaa
     3121 aataacaggc aacccgaatg aggaaaaatt ttccgcgaaa attttccaac tttattattt
     3181 tttcgcgcgt cataatttac ggactgactc aatttattga gttttcgtcc gaaaaaatta
     3241 cgtactaggg gagaaaaaaa aatgaaaaaa cttttaacga tttcggcact cttaggctca
     3301 ctgataattt tcgcgccttc acaggaagca aaagcggcaa gcgcggcaag cattgccgag
     3361 ccgcagattc gaattcagct cggacaggac cgtcgctggc gcaatcgtcg ctcgaccatc
     3421 cagacgcgcg tcgtccgtcg cggatttgct acctatcgtg aaacttatcg agtcacttac
     3481 agacgaaacg gaagtgttcg cacagagctt atcagccgcg tcagaattgg cggtcgtcgc
     3541 tactcttact aaaagcttag agcgggaatc tactctctcc tcccgactcc tttatttcgc
     3601 ctctttcccc gaaaattccc gcgccgtttc cagataattt cctttatctt ccagattcgc
     3661 cataaactgc ctgaactctt ccgatttgtt tttcggaatg ctcaaagttt tccaatcgct
     3721 cgaacgaaaa tgtttcgcca gcagagttat ctgcccgacg tgctgaccgc tgtgcgaaag
     3781 cgcgcgataa atcgctttga cgaccgtaaa atcttcggtg cgaattttta cgattttgcc
     3841 caaatcgtcc gttcttaaag attcgagcgc ctgaaacaaa gcccgccagc cgctttccca
     3901 agcctgcatc aaactttccc gcgtgtcgtt ttccgtgacg aattcggaat cgcggtgacg
     3961 gtcagccttt tcgccgtccg ttgtcaaaaa gcgcgtccag cgcgagcgca gatttccgcc
     4021 caaatgcttg acaagcgcgg caatcgaatt agcttcggcg tcaatcagtc taaaaaactc
     4081 gtcgtcggaa acctgctcaa tcgcgccttc cgccagtttt ttctgaccgc gaaacgcagc
     4141 gatagcttcg tccaaaacgt tttgtaaaat ttgttcgctc atattattta attttaggat
     4201 aagacggaat ccattccaaa gccgtgtcgc gtcctttcag ataagatttc caagataata
     4261 aattcggaca accaactgtc ttataaacga acagttttga gccggcttta tactttatcc
     4321 tgtgcgattc ggtcaaaatg ccttattaat ttatcaaaaa ttatttaatt cagatgcggc
     4381 acaggcattg cagttattcc cgagcaacat ctgtattgtc tgattttgat gcagttctta
     4441 aaaatcggtg atacgcgtaa taaataaaaa caatcaaaag ggagataaaa attatgaacc
     4501 aatcaacagg acaatcacgt ttagtgtcgg gtctttttaa tgacagacaa agcgcggaga
     4561 gagtttattc atcgctggaa agccgcggtt attcgaaaga cgacgtcaat ttaatgatgt
     4621 ctgatgaaac gcgcaatcgt catttcgggg aaggaacgcc tgaaacggaa cttggcagca
     4681 aagcgctgga aggcgcgggc gcgggttcgg caatcggcgg aacactcggc gcgattatcg
     4741 gtggtattgc cgctattgga acgaatgttt tgcttccggg tttaggatta gttgtcgccg
     4801 gaccgatttt tgcggcttta gcgggcgcgg gcgcgggcgg cttgacgggc ggcttggtcg
     4861 gcgcgttaat cggttcgggc attcccgaag aacacgctgc cgaatatgag tcgggaatca
     4921 aaaacggcgg cgtttatatg ggcgtcaatg cgcgtaacga tgaagacgca caatatttcc
     4981 acgaagaatt taatcgtcac ggcggctcgc acgtcgtcgg cacgggactc ggcgtagcag
     5041 gcggcgcggc aaccggagca gctatcggca ccgccatcgc gcccggcgtc ggcacggtcg
     5101 gcggcggaat agtcggagcg gttgcgggcg gaatcgccgg cggcgctgcc ggacacgaaa
     5161 tagcggaaaa agtaaatccc cagccgggag acgagcacga tggtgaccac aacgtcggaa
     5221 caggaaccgg cgcagtcggc ggagcagtta ccggcgcggc aatcggttcg gttgttcccg
     5281 gcgtcggcac ggcagccggc ggagttgtcg gcggagttgt cggcggagtc ggcggagcgg
     5341 tcgccggtca cgaagtggct gaaaaagtaa atcccgacga caagttaatt gatgacgata
     5401 acgtgaccag aagataaatg cttcagaaaa gcaggtcaag ggaagcttct aatgaggctt
     5461 cccttttttt atgtgtttta tgccgggtct ttactattct atgccggacg actgcgttaa
     5521 cgtaatcgcc cgaacgagac gatttaaata tacgaaacgc tgcgaagaac attttcccga
     5581 tttaatcgcg gcataaattc taagcttgaa ttttcttcgc aatatcttcc cttaaaagcg
     5641 tttcctgcgc gcccgactgc atatttttga gcgtcacttt attttccgcc aattccgttt
     5701 cgcccaggac gcagacgaac ggagcgttga tttgcgaagc gtatttgaat tgttttccga
     5761 gcttgtcggc ttcgggataa accagaacgc gcaagccctt cgagcgtaaa tcattggcga
     5821 gtttgagcga ttcgccgact gagtcatcgt tccagattgt caccaaaaca tcagccggag
     5881 cgttctgcaa atcttcggga aacatatttc tctcggtcat cacgacgata attctctcca
     5941 agcccaaaga aaaaccgcac gccggaatct gctccttgcc gaacatcccg atgagttcgt
     6001 cataacgacc gccgccgccg atactgcccg cgaaatcgcc gcccgttaaa acgatttcca
     6061 taatcgctcc cgtgtagtaa gacaagccgc gagcgagcga agcatcgatt tcgatatttt
     6121 ccaaaccggc aagaccgaga atttgcgcta attcatttaa accgtttttt cccgtttcct
     6181 gttcgccgac gaattcggca aggcgcgaga gcttgtcggt attgcccgcc gtctccaccg
     6241 cggcgaataa atcgagcaat ctcagtcccg cgctttcgtc gatgtcgcgc tgcacaaatt
     6301 cttttaaaac gccgtctttc ccgattttat cgagcttgtc caaagcaatc agcgcgtcgg
     6361 actgcttatc cgcttcgatt tggacggctt ctaaaatacc gcgcagaata tctctgtggt
     6421 tgaggcgcac gacaaaatca ttaaatccca atcgctgtaa aatctccgat gccgcagaaa
     6481 ttatttccgc ttcgataatc attgaatttg agccaatggc gtccacatcg cactggaaaa
     6541 actcgcggaa acgcccgcgc gcggggcggt cagctcgcca gacgggttgg atttgatagc
     6601 gtttgaaaaa cttcggcaaa tcgtttttgt gattcgccac gacgcgcgcc agcggcacgg
     6661 tcaaatcata gcgcagggcg aggtcggata gctctttgtc gtcggcgttg agcttttcgc
     6721 cgcgtttgag gattttgaaa atcaattgat tgccttcctc gccgtatttg cccatcagcg
     6781 tttcgaggtt ttcgacggct ggcgtctcca gcggctcgaa gccgtaagat tcgtaaacct
     6841 gcttgataat tccgataacg tattcgcggc ggcgcacgtc aacgggcaaa aaatcgcgca
     6901 tcccgcgcgc cggattcgta ttgctcattt tctttactgc tcctcaagat ttcgctcggc
     6961 gacggcggct aaattttcgt aattgtcagc cagtctttta atatcgtcgt acaaaaatcg
     7021 gtcgttattc agaaaatcct tttcgcgata atccgggacg gcgtttttta tttcctcgaa
     7081 taattctccg gtcgcttttc ccattccggt ttccagtcct aatttttcaa actgattgcg
     7141 gcgaatttcg gcggcgtgat gcgccgtcag aacggcgatg acgacgacgt ttttcacgtt
     7201 ttcgagaatt tgcagagcct gtctgcccgc cgtcgcgccc atactgacgt ggtcttccgt
     7261 attcgccgag gtcggaatcg aatccgccga agccggatgc acgagaacct tgttttcgga
     7321 aactaaagac gcggcgacgt attgcgtaat catcaagccg gaattgatat ttttttcgtc
     7381 gtaaaccaga aacggctcga gcaaatcgtt ggtcgccttg tcgtgcagtt tattgatttg
     7441 acgctcaacg aggtttccga cttccgaaat cgccagcttc atataatcga gcgcgaaagc
     7501 cagcggctga ccgtgaaaat tcccgccgga gagcactacg tcctcttcga cgaaaatcag
     7561 aggattatcg acggcggcgt tcagctcgtt ggcgaaaatt cctttcgcgt gatttaagac
     7621 ctgcaaactg gcgccgaaaa cctgcggcat acagcgaatc gaatacgaat cctgcgttct
     7681 gcctttcagg tcggaaagtc tttcggcgaa atcctttttc ctttccagcc aatcggtctc
     7741 ttcgacctcg attcccgcgg ttttcgattt ggcacgcaaa gcttcgctcg aaatgcctat
     7801 aaaagaagaa ttttccgtaa atttccgaat ccagccggcg accgtttcct gctcggcgtg
     7861 atgccgaacc cggtgaattt tttcgtcgaa agcgtttcgc cgcgcgcaga aggcttcgag
     7921 catcagcgaa gagaccagag tcgccagctt taaaagccgc tcgaattcgt aaacggcaaa
     7981 cgtgccgagc gccgccatca cggtcgtgcc gttgtttaaa gccaagcctt ccttgtagct
     8041 taattcaaac ggtttcaaaa gattgtcttt tgcttctttc aagccggaaa attcgtccgc
     8101 tttcagacgt tccagaaatt ccgcggtttt gtattccgcg cctttgtatt cgacgtagcc
     8161 ttcgccgatt aaaggcaaag ccagatgcga gagcggacac aaatcgccgc tcgcgccgag
     8221 cgagccctgt tcgggaacga gcgggtgaat ttgaaaattg agaaaatctc tgagccgttc
     8281 gagcgtgtcg actttgacgc ccgaatagcc tttcaccaaa gtattcaggc gaatcagcat
     8341 aatcgcgcgc acaacgcgcg tcgggaaatt tttgccgacg ccggtcgcgt gcgagcgcag
     8401 gagattgatt tgcaattgtt tggcgtcttc cggcgcgata atctggtcgg ctttggctcc
     8461 aaagccggtc gtcacgccgt agattattgc gttttccgcc acttttcgag cgataaagtc
     8521 acggcttttc cgcacttttt ccagattgcc taattcgacc tgaacgtttt ggcaggcgac
     8581 cttgacgaca tctccgagag tcagagcgtc gcccgtaatt gtaatttttt ccatactcgc
     8641 ttgcgattcg ctgttttcgt cagag
//
LOCUS       DASWNE010000315         2468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_10457, whole genome shotgun sequence.
ACCESSION   DASWNE010000315 DASWNE010000000
VERSION     DASWNE010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10457"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..123
                     /locus_tag="VGB68_06485"
     CDS             <1..123
                     /locus_tag="VGB68_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_061524768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEY0048911.1"
                     /translation="FNGVSKQTFYLHLKECEWRFNNRSKNLYLELLKLLRENPL"
     gene            197..541
                     /locus_tag="VGB68_06490"
     CDS             197..541
                     /locus_tag="VGB68_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048912.1"
                     /translation="MKINYDNRIFKSISNSETGEVSRDTTFHYHQKDNLVWAEYAGGE
                     IVFGSLIAKVLPDDSLEMRYQHLNEKGELMTGKCFAKPEILPDARIRLYEKWHWTSGD
                     FSAGESIVEEII"
     gene            complement(789..1013)
                     /locus_tag="VGB68_06495"
     CDS             complement(789..1013)
                     /locus_tag="VGB68_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048913.1"
                     /translation="MNKDAIKIIKRNKKNAAEKARAGVEAPKAESDARHGIVNTVENW
                     ISERRENRREEKVFSDDKISGWETTSKNSK"
     gene            1534..2280
                     /locus_tag="VGB68_06500"
     CDS             1534..2280
                     /locus_tag="VGB68_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY0048914.1"
                     /translation="MPVTKHTDNAHLNHLLAALPADEFARLSPDLEPVSMSLGEVLYE
                     SGDKMTHIYFPTTAIISLLYIMENGATAEIGIAGNNGLIGIALFMGGETTSSRAVVQS
                     AGDAVRMKAGDLKTAFALGGVFQNILLRYTQSLMTQISQTAVCNRLHSVEQQLCRWLL
                     INHDQLQTDKLVMTHDLIANMLGVRREGVSIAAANLQKKGLIKYVRGTITVLDRKGLE
                     AAACECYQVVKDEYDRLLGEYIPKNQESIK"
BASE COUNT          675 a          563 c          550 g          680 t
ORIGIN      
        1 ttcaacggcg tctcgaaaca aactttctat ctgcacctga aagagtgcga atggcgtttt
       61 aataatcgtt caaagaactt gtatcttgaa ttgttgaagt tattgagaga aaatcctctc
      121 taaacttccc tagaccctta attttttatc ctgtttatcc tgtccatcaa gccgcgaagc
      181 ctactttgaa tttaccgtga aaattaatta cgacaaccgc atttttaaat ccatttccaa
      241 ttccgaaacc ggcgaagttt ctcgggatac gacgtttcat tatcaccaga aagataatct
      301 ggtctgggcg gaatacgcgg gcggcgagat tgttttcggc agtttgatcg ccaaagttct
      361 gccggacgat tcgctcgaaa tgcgctacca gcatttgaac gaaaaaggcg aattaatgac
      421 cggaaaatgt ttcgcaaagc cggaaatttt gcctgacgcc cggataagac tttacgaaaa
      481 atggcattgg acgagcggcg acttttcggc gggcgaatca atcgtggaag aaatcattta
      541 accgtcaata tgctttgcac aaaaataaag aaaatcgaga aaaattccga tggctaattg
      601 taaattctaa attattaatt ctaaattatt taacaaaaga taccaaacat caatttagaa
      661 tttacaattt tgaatttaca attttccctt tctttattct tgtgcaaagc cgattcaggt
      721 tgttctgcgc gttcgatata tcgccgtata tcaataaaaa tttcctgtgc cgcattattc
      781 tttgtcgttt atttggaatt tttggacgta gtttcccagc ccgaaatttt gtcgtccgaa
      841 aaaacttttt cctcgcgcct gttttcgcgt cgttcggaaa tccagttttc aaccgtattg
      901 acgattccgt gtcgagcatc gctttcggct tttggagcct cgactccagc ccgcgctttt
      961 tccgccgcgt tctttttatt tcgtttgata attttaatcg cgtctttatt catagattag
     1021 aatgccgggc tgattaacaa tcaataagta aaaaaaacga aggatggctt tccacttaat
     1081 gaaagccatc cttcaaaaca gctttcgcta ttaaaacata gtgattgcag gaaaaggagt
     1141 taactatgtc aaataagcct tcgtcatttg cattcgtaaa caaaagcgcg gacgaaacaa
     1201 gcgggtggaa tgcttgaatt tcgttcaatg actgagccgt atcataaatt aaccgtttga
     1261 aaaagattta tccgtgcgtt tccgcgcata taaaaaaaat tatccgtgcg ccggcatccg
     1321 gctgagagcg gcgcgccgga ttttatttgt attcgggcgt atgagaagct cagcctgcgg
     1381 caaaacgcca acgtttctgc gctcgcctgc ttataaaacc ttttgaagga ctgtgcgcca
     1441 gcgcacacaa aatcgcttcg tttgtgataa tctttccata acaggttatt aaccggaacg
     1501 atttcactct ctctcagcca tataggaaga attatgccag tcaccaaaca taccgataat
     1561 gcgcatttga atcatctgct ggctgcgctg cccgccgatg aattcgcgcg cctgagtcct
     1621 gatttagagc cggtttcgat gtctttgggc gaagttcttt atgaatcggg cgataaaatg
     1681 acgcatattt attttccgac gacggcgatt atctcgctgc tttatattat ggaaaacgga
     1741 gcgaccgccg aaatcggaat agcgggcaac aacggtttga tcgggattgc gctttttatg
     1801 ggcggcgaaa cgacgtcgag ccgggcggta gtgcagagcg cgggcgatgc ggtgcgaatg
     1861 aaagccggag atttgaaaac cgcgtttgcg ctgggcggcg tgtttcaaaa tattctgctg
     1921 cgctacaccc aatctttgat gacgcagatt tcgcaaaccg ccgtctgcaa ccgcctgcat
     1981 tcggtcgaac agcagctctg ccgctggctg ctcatcaacc acgaccagct gcagacggac
     2041 aagctggtga tgacccacga cctgatcgcc aatatgctcg gcgtgcgccg cgagggcgtt
     2101 tcgatagccg ccgcaaactt gcagaaaaag ggattgataa aatatgtgcg cggcacgatt
     2161 accgtgctcg accggaaagg cttggaagcc gccgcctgcg aatgctacca ggtggtcaaa
     2221 gacgaatacg accgcctgct cggcgaatat atcccaaaaa accaggaatc gattaaataa
     2281 tccgctccgc gccgaaaaaa aacaaaaatc agatgtttta gtatgtgcgg cgacgcacgg
     2341 acataagtgt atttatctgt tatcatattc gggtaaaagt tattttacaa ttcaccattc
     2401 gacatcagga ttggtctagc caacttcttt ttgtgccgtc tgctgttttc gcaacacgac
     2461 tgaaaagg
//
LOCUS       DASWNE010000316         2164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_151194, whole genome shotgun sequence.
ACCESSION   DASWNE010000316 DASWNE010000000
VERSION     DASWNE010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151194"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VGB68_06505"
     CDS             <1..544
                     /locus_tag="VGB68_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048915.1"
                     /translation="FVAHPEYTEILKDYDELVDMEAIGEMEVFVKELRRKLPIAEFLD
                     GVSSKEERSKKDTGERYITMVDKQYNINNAQIASLGDKNKVSGNTFNQQNIANENIDF
                     ELLSEELKRLREVLAAKATEAEHYIAIGEVANAEKAAEEKDGNKVVGYLKSAGSWVLD
                     SAKEIGVDIVTDLIKKQIEP"
     gene            558..1331
                     /locus_tag="VGB68_06510"
     CDS             558..1331
                     /locus_tag="VGB68_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048916.1"
                     /translation="MKQETTHNHQTATAEKIFKDREVWVGTFLGGPLVAGYMIAHNFK
                     TFGDGARARKTWIIAFAATLALFGIALFAPYIERIPQVAIPLVYTGMAFVLVRLYQGE
                     KIAVFVGAGGRIHSWWRTIGVTIAGMVITFVPFLGAAYAVEAVRNANVVAKKYGASGH
                     EIAFDKTNLAESEIDALAAGFEQNNFFDDGGSWYVYVRKIENSYEIFVSVERGISNRP
                     QDVKLFARLRDNMQKLFPNNRIVFNLVVDDLDDVVRRLE"
     gene            complement(1496..>2164)
                     /locus_tag="VGB68_06515"
     CDS             complement(1496..>2164)
                     /locus_tag="VGB68_06515"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HEY0048917.1"
                     /translation="AKEKGEKINFGDATRREVLHHAHIENANALVLAMSDPHAARRTV
                     KQARELNENIYIVVRTRYTSEITELLELGADEVIPEEFETSIEIFSRVLLRYGVDRHT
                     IESQIERIRRQGYEMLRSPSLQRVQMANLNAALESATTENIKLNENSPVVGKTLGELN
                     LRNQTSATLIAVVRGEETKIGPGANYKLQTNDILILFGKPESIERAIKILHPDEELTG
                     GFNP"
BASE COUNT          531 a          497 c          619 g          517 t
ORIGIN      
        1 atttgtcgcg catcctgaat atactgaaat tttgaaagat tacgacgagc ttgtcgatat
       61 ggaagcaatc ggcgaaatgg aagtctttgt gaaggaattg agacgcaaac ttccgatagc
      121 ggaatttctg gacggcgttt ccagcaagga agaaagaagc aaaaaagata cgggagaaag
      181 atatataact atggtcgata aacaatacaa cattaacaac gcgcaaatag cctcattagg
      241 cgataaaaat aaagtttccg gcaatacttt taatcaacag aatatcgcca acgaaaacat
      301 tgatttcgag cttctttcag aagaattaaa aagacttaga gaggttttag ccgcgaaagc
      361 caccgaagcg gaacactata tagcaatcgg cgaagtggcg aacgccgaaa aagccgccga
      421 agaaaaagac ggcaataagg tagtcggcta tctgaaatcc gcaggcagtt gggtgctaga
      481 ttcggctaag gaaataggcg ttgacatcgt aacggactta ataaaaaaac agattgaacc
      541 ctaattcgga aagacaaatg aaacaggaaa caactcataa ccatcaaaca gcgacggcgg
      601 agaaaatttt taaggacaga gaggtctggg tcgggacgtt tctgggcggt ccgttggtgg
      661 cgggttatat gattgcgcat aactttaaga cgtttggcga tggggcgcgg gcgcgcaaga
      721 cgtggattat cgcttttgcc gcgacgctgg cgcttttcgg gattgcgttg tttgcgccgt
      781 atatcgagcg aatcccgcag gtggcgattc cgctggttta tacgggaatg gcttttgtgc
      841 tggtgcgttt gtaccagggc gaaaaaatcg cggtttttgt cggcgcgggc gggcggattc
      901 atagctggtg gcggactatc ggggtgacga ttgccgggat ggtcattact tttgtgccgt
      961 ttctgggcgc ggcttatgcg gtggaagcgg tcaggaacgc caacgtcgtc gctaaaaagt
     1021 acggcgcgtc gggacacgaa atcgctttcg acaaaacgaa cctggcggaa agcgagattg
     1081 acgcgctcgc cgccggattc gagcaaaata atttctttga cgacggcggc agctggtatg
     1141 tttatgtccg caaaatcgaa aacagctacg agattttcgt ttccgtcgag cggggaattt
     1201 cgaatcgccc gcaggatgta aagcttttcg cacggctgcg cgataatatg caaaagctgt
     1261 ttccgaacaa caggattgtt ttcaatctcg tcgtcgatga tttagacgac gtggtcaggc
     1321 gactggagtg agaacaagta aaaaagccgg ggagaagatc gagcagattc ggcaactgcc
     1381 tttctcctaa acttcccggc ttctctactt tattcagcgc ctgcccggcg cgcgggcggc
     1441 tgccttttct tccgccggaa aacccgcaac tccaacaatt atcgaaaaat tctctttaag
     1501 gattaaaacc gcccgtcaat tcttcgtcgg ggtgtaaaat cttgattgcc cgctcgatgc
     1561 tttcgggttt gccgaataga atcaggatgt cgtttgtttg aagcttatag tttgcgccgg
     1621 gaccgatttt cgtttcttcg ccgcggacga cggcgatcag cgttgcgctg gtctgattgc
     1681 gcagattcag ctcgcccaga gtctttccga cgaccggaga gttttcgttc aatttaatat
     1741 tttcggtcgt cgccgattcg agcgcggcgt tcagattcgc catctggacg cgctggagcg
     1801 agggcgagcg gagcatctcg tagccctgcc ggcgaatcct ctcaatctgg ctttcgatgg
     1861 tgtggcggtc gacgccgtag cgcagaagga cgcgcgagaa aatctcgatg ctcgtctcga
     1921 attcctcggg gatgacttcg tccgcgccga gctcgagcag ctcggtaatc tcggacgtgt
     1981 agcgcgtgcg gacgacgatg tagatatttt cgttcagctc gcgcgcctgc ttgacggtgc
     2041 ggcgcgcggc gtgcgggtcg ctcatcgcca gaacgagcgc gttggcgttt tcgatatgcg
     2101 cgtgatgcag aacctcgcgg cgcgtggcat cgccgaaatt gattttctcg cctttttctt
     2161 tggc
//
LOCUS       DASWNE010000317         1687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_503934, whole genome shotgun sequence.
ACCESSION   DASWNE010000317 DASWNE010000000
VERSION     DASWNE010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503934"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VGB68_06520"
     CDS             <1..463
                     /locus_tag="VGB68_06520"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEY0048918.1"
                     /translation="DEAETERRLEHYYRPYYQKIESLLKDRIEKFGQALLWDAHSIRR
                     FVPTIRKEPFPDLILGDNDEQSAAKEIIDIALGSLRAGKYQVNHNTPFKGGHITRYFG
                     RPETRVHALQLEMCKTHYMNDDELAFNEDRAREMRKLLHPAFEKLIAHLTK"
     gene            460..>1687
                     /gene="hutF"
                     /locus_tag="VGB68_06525"
     CDS             460..>1687
                     /gene="hutF"
                     /locus_tag="VGB68_06525"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050416 - formimidoylglutamate deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0019557 - histidine catabolic process to
                     glutamate and formate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HEY0048919.1"
                     /translation="MKSYRFKALLQNRGWLENATVSLNESGEIVSVSQAENADSIYID
                     GYALPGFQNAHSHAFQYAMAGLAERHSAAAPAADDFWSWREAMYRLASNISPEQTKAI
                     AAMLYAELLRNGFTNVAEFHYVHHAPSGEPYDNLAEMGAALVEAAQEAGIKITLIPIF
                     YQKGGFGIPADERQKRFISATFDDYLKLFDASAQICRRYEGASAAVGIHSMRGVRHQD
                     ILRAATEAPTDVPFHIHVAEQLKEVEDALAYLGKRPVEWLLDNVGLNERFHLVHATHL
                     TENETERLAKTGANVVLCPSTEGNLGDGIFPLRDYQSFGGQWSIGTDSHVGLNPLEEL
                     RILDYGQRLITHRRDTFIGDNIEDSGLYAITRATAAGRRAMNNFEPEFFAAGASFDAC
                     IINADEPLLANVKTESL"
BASE COUNT          435 a          411 c          462 g          379 t
ORIGIN      
        1 ggacgaagcc gagaccgaaa gaagactgga gcattattat cgcccttatt atcaaaaaat
       61 cgaaagtctt ttaaaagaca gaatcgagaa attcgggcag gctttattgt gggacgcgca
      121 ttctattcgg cggtttgtgc cgacgattag aaaagagcct tttccggatt taattttggg
      181 cgacaacgac gagcagtccg cggcgaaaga aattatcgac atcgctctcg gcagcttgcg
      241 ggcgggaaaa tatcaggtca atcacaacac gccgtttaaa ggcgggcata ttacgagata
      301 tttcggcaga ccggaaacgc gcgttcacgc ccttcagctg gaaatgtgta agactcatta
      361 tatgaacgac gacgagctgg cgtttaatga agaccgcgcg cgggaaatga gaaaactgct
      421 gcatccggcg tttgaaaaat taatcgctca tttaacgaaa tgaaatccta ccgcttcaaa
      481 gcgctgctgc aaaatcgagg ctggctcgaa aacgcgaccg tttcgctgaa tgaatccggt
      541 gaaatcgtct ccgtttcgca ggcggaaaac gcggattcca tttacatcga cggctacgct
      601 ttgcccggtt ttcagaacgc gcattcgcac gcttttcaat acgcgatggc tggcttggcg
      661 gagcggcatt ccgccgccgc gccggcggcg gacgatttct ggtcgtggcg cgaggcgatg
      721 taccggctcg cttcaaatat cagtcccgag cagacgaaag caatcgccgc gatgctctac
      781 gccgaattgt tgcgaaacgg ttttacaaat gtggcggaat ttcattacgt tcatcacgct
      841 ccgagcggcg agccttacga taatttagcc gaaatgggcg cggctctcgt cgaagcggcg
      901 caggaagcgg gcataaaaat cactttaatc ccgatttttt atcagaaagg cggtttcggg
      961 attcctgccg atgaacggca gaaaagattt atctccgcaa cctttgacga ttatttaaag
     1021 ctttttgacg cttccgcgca aatttgcaga cgatacgaag gcgcgagcgc ggcggtcggc
     1081 atccattcga tgcgcggcgt ccggcatcag gatattttaa gagccgcgac ggaagcgcca
     1141 acggatgttc cgtttcacat acacgttgcc gaacagttga aagaagtcga agacgcgctc
     1201 gcgtatctgg gaaaaagacc ggtcgaatgg ctgctcgata acgtcgggtt gaatgaaaga
     1261 tttcatctgg ttcacgccac gcatctgacc gaaaacgaaa ccgaaaggct ggcgaaaacg
     1321 ggcgctaatg tggttttgtg tccgagcacg gaagggaatc tgggcgacgg gatttttcct
     1381 ctcagagatt atcaatcttt cggcggtcag tggagcatcg gcacggacag tcacgtcggt
     1441 ttaaatccgc tcgaagaatt acgaatcctc gattacgggc agcgtttaat cacgcacagg
     1501 cgcgatactt ttatcggcga taatatcgag gacagcggct tatacgcgat taccagagcg
     1561 accgccgccg gacgccgggc gatgaataat ttcgagccgg aatttttcgc cgccggcgct
     1621 tcgttcgatg cctgtattat caatgccgac gagcctctgc tggcaaatgt aaaaacggaa
     1681 agtttaa
//
LOCUS       DASWNE010000318         2444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_434375, whole genome shotgun sequence.
ACCESSION   DASWNE010000318 DASWNE010000000
VERSION     DASWNE010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434375"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..359)
                     /locus_tag="VGB68_06530"
     CDS             complement(57..359)
                     /locus_tag="VGB68_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048920.1"
                     /translation="MLEHLTRDQFAEHLNGKFKIYVTPETAVEAELTEVSEIRKRPRQ
                     EAFSLLFLALTDTPFEQAGYKIEHPVLGSDILFLVPIEKTARGVFYEVLFNRLTDD"
     gene            complement(413..1189)
                     /locus_tag="VGB68_06535"
     CDS             complement(413..1189)
                     /locus_tag="VGB68_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048921.1"
                     /translation="MPDVTTANSDSSNLFFENLPENFVFPGDALGRRLLREYGSVFVA
                     RGGVVAPLTVIYENEREVAKFQSSVGKSRKIIGGFEIELQTAAMLALKKAAGEAEKND
                     LNITPRGGDAARRSYSETVDLWASRVNPALAHWVEKGRLTRLQASRIKSLAPVLQISE
                     VLELEERGIFFSKDFSKSILYSVAAPGTSQHLSMLALDIAEHDNAGVRRILANHGWHQ
                     TVISDLPHFTYLGAAESDLPRLGLKKSSVGGDRDFWIPAL"
     gene            1252..2001
                     /locus_tag="VGB68_06540"
     CDS             1252..2001
                     /locus_tag="VGB68_06540"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY0048922.1"
                     /translation="MKRFGQIIKRIFPVIYLLALHAFAALFIFEKFIAGYIYPDNSLF
                     PNVRVQTEETPAPSPLVVPSIIDVNVNAADNTLADSETNINTNANQISNQNNSSYSAP
                     AGALMIPVAGVRREQLTDTFAASRSGGRVHDAMDIMAPLGTPVVAAADGEIAKFFDSK
                     LGGITIYQYSADKKYVYYYAHLQKRADNLKEKDFVKQGTVIGYVGDSGNAGAGNYHLH
                     FSISIPADPARYFEGNYINPFPMLKNGIEPR"
     gene            2174..>2444
                     /locus_tag="VGB68_06545"
     CDS             2174..>2444
                     /locus_tag="VGB68_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048923.1"
                     /translation="MKLDQSKIFALFFTLILFSFYGCAPQQQGNTNTAVNINTNPENT
                     TVATNTNVAKPAENTEAAQAVPVTLPVLDAMFGDEQFAGELKTKLQ"
BASE COUNT          637 a          636 c          556 g          615 t
ORIGIN      
        1 tttcgctttt cacttttcgt gtgtttcggc ggtcaaattc ttaaatccga ctaacactaa
       61 tcatcggtca ggcgattaaa taaaacttcg taaaaaacgc ctcttgcggt cttctcgatc
      121 ggcaccagaa acaatatatc cgagccgaga acgggatgct ctattttata accggcttgt
      181 tcaaacggag tgtcggtcag cgccagaaaa agaagcgaaa aggcttcctg gcgcgggcgt
      241 tttcttattt ccgaaacttc cgtcaattcc gcctcgacgg ctgtttccgg cgtcacgtaa
      301 atcttaaatt taccgttcag atgttcggca aattggtctc tggtcagatg ttccaacatt
      361 tttgtttttc ctctcaataa aaattcgctg cttcggtaat tctaaagttc ggctaaagcg
      421 ccggaatcca aaaatcccgg tcgccgccaa cggatgattt cttcaagccg agcctcggca
      481 aatcgctttc cgccgcgccg agataggtaa aatgcggcaa atccgaaatg accgtctgat
      541 gccagccgtg atttgccaga atccggcgca ccccggcgtt atcgtgttcg gcgatgtcca
      601 gcgccagcat cgaaagatgc tgcgacgtgc cgggcgcggc gaccgaatac aaaatcgatt
      661 tcgaaaaatc cttgctgaaa aaaatcccgc gttcctcaag ctctaaaacc tcggaaatct
      721 gcaggacggg cgcaagcgat ttgatgcggc tagcctgcaa ccgcgtcagg cgacctttct
      781 cgacccagtg cgccagagcc ggattgacgc ggcttgccca caaatcgacc gtctcggaat
      841 aactgcggcg ggcggcatcg ccgccgcgcg gcgtaatgtt taaatcgttt ttttcggctt
      901 cgcccgcggc tttttttaat gcgagcatcg ctgccgtttg cagctcgatt tcaaagccgc
      961 cgataatttt tcgtgattta ccgacggaag attgaaattt tgctacttcg cgctcgtttt
     1021 catagatgac ggtcagaggc gcgacgacgc cgccgcgagc gacaaaaacc gacccgtatt
     1081 ccctgagcaa tcttcgaccg agcgcgtcgc ccgggaaaac gaaattttcg ggaagattct
     1141 cgaaaaacag atttgatgaa tcgctatttg cggttgtgac atccggcatt aatttatttt
     1201 ttacggataa aaatcttgtg gttattattt ttacctcata caatttacct tatgaaaagg
     1261 ttcgggcaaa ttatcaaaag aatttttccg gtgatttatt tactggcgct gcacgccttc
     1321 gccgcacttt ttatctttga aaaatttatt gccggttata tttatcccga taattcgctt
     1381 tttcctaatg tccgcgtgca aaccgaagag acgcctgctc cgtcgccgct ggtcgtcccc
     1441 tcgattatag atgtaaatgt caacgccgcc gataatacgc tcgccgattc agaaacgaat
     1501 attaatacaa acgccaatca aatttccaat caaaacaatt cttcgtattc cgcaccggcg
     1561 ggcgctctga tgattccggt cgccggcgtc agacgcgagc agctgaccga cactttcgcg
     1621 gcttcgcgct ccggcggtcg cgttcacgac gctatggata ttatggctcc tctgggaacg
     1681 ccggtcgttg ccgcggctga cggcgaaatc gccaaatttt ttgacagcaa gctcggcggc
     1741 atcaccatct atcaatacag cgccgacaaa aaatatgttt attattacgc ccacctgcaa
     1801 aaacgcgccg acaatctcaa ggaaaaagat tttgtcaaac agggcacagt tatcggctac
     1861 gtcggcgatt ccggaaacgc cggagcggga aattatcatc tgcatttctc gatttcgatt
     1921 cccgccgacc cggcgcgcta ctttgaaggc aattatataa acccgtttcc aatgcttaaa
     1981 aacggcatcg aaccccgctg aaaaaggctt taaaaaacga aaagcgaaaa cgctattcgc
     2041 ttttcactaa aaaaaatttc ttcgttccaa tcaaattcgg caaaggaata actattgctt
     2101 tacgcttact gtcggtaacg ttcgtgtatc gaaacagaac gcatctcaga ggagcagaaa
     2161 agaatttata gttatgaaat tagaccaatc aaagatattc gccttatttt ttaccttaat
     2221 cttatttagt ttctacggct gcgcgccgca gcaacagggt aatacgaata ccgccgtaaa
     2281 cataaacacc aatccggaaa atacgaccgt ggcgacgaat accaacgtcg ccaaaccggc
     2341 ggaaaacacg gaagccgcgc aggcggttcc ggttacgctg ccggttttgg acgcgatgtt
     2401 cggcgacgaa caatttgcgg gcgagctaaa gacgaagctg caat
//
LOCUS       DASWNE010000319         3479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_293123, whole genome shotgun sequence.
ACCESSION   DASWNE010000319 DASWNE010000000
VERSION     DASWNE010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293123"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VGB68_06550"
     CDS             complement(<1..225)
                     /locus_tag="VGB68_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048924.1"
                     /translation="MRKRTPQEKKALSYARDRRNDYGENDKGSRKSIRRNKTFPNRAY
                     RKTVGDILRGAAGAVDASQAESLDFKTREIR"
     gene            complement(225..1664)
                     /gene="sufB"
                     /locus_tag="VGB68_06555"
     CDS             complement(225..1664)
                     /gene="sufB"
                     /locus_tag="VGB68_06555"
                     /inference="COORDINATES: protein motif:HMM:NF008773.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEY0048925.1"
                     /translation="MSTATELLTNREYKYGFVTDIETETIAKGLSEDTVRLISSKKNE
                     PEWMLEFRLKAYRHWLKMKQPDNWANFNYPNIDFQNISYYSAPKQKAKKQSLDEVDPE
                     LLKTFEKLGIPLTEQKMLANVAVDAVFDSVSVATTYKKKLLEAGVIFCSFTEAVADYP
                     ELIKKYLGSVVPVSDNYYAALNSAVFSDGSFVFIPKGVRCPMELSTYFRINTQESGQF
                     ERTLIVAEEGGYVAYNEGCTAPQFDTNQLHAAVVELVALDDAEIKYSTVQNWYAGDES
                     GKGGIYNFVTKRGACRGKNSKISWTQVETGSAITWKYPSVILQGDNSIGEFYSVALTN
                     NAQIADTGTKMIHLGKNTKSTIISKGISAGRSNNSYRGLVKIMPRASGARNYTQCDSM
                     LIGGKSEANTFPYIEVMNNTARVEHEATTSKISEEQLFYLQQRGISQEDAVSLIINGF
                     CKEVFRELPMEYAVEATKLLGLKLEGSVG"
     gene            complement(1742..2221)
                     /locus_tag="VGB68_06560"
     CDS             complement(1742..2221)
                     /locus_tag="VGB68_06560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY0048926.1"
                     /translation="MKISAQEEYGLRCLVQLATLSGNESRTLPQIAELEGISVANAGK
                     LMWLLNKAGFVQATRGTKGGYSLARPASEIRLNEVIKVLDEDVLNKHCESYTGVLETC
                     VHKGDCGIRPVIVGLHEIVENALSQITLAQLVGTESAVDAMFHQIKGIQRTIEPQRI"
     gene            complement(2475..2768)
                     /locus_tag="VGB68_06565"
     CDS             complement(2475..2768)
                     /locus_tag="VGB68_06565"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0048927.1"
                     /translation="MGLKPRLRAERLPEKLKKLREELGLSQNEILIQLGFADRFDRST
                     ISHYETGEREPPLPILLRYAQLANIYVDVLIDDKSDLPNKIPCKVKSEGRELS"
     gene            3104..>3479
                     /locus_tag="VGB68_06570"
     CDS             3104..>3479
                     /locus_tag="VGB68_06570"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HEY0048928.1"
                     /translation="MKTIPSLLLSITILLFACCLVFAQENRWSYVGTDASGTRFFIDR
                     NSIEIKGNRLRVWNKFFYANQTYRLSLFEWACAEKKYLILDETSYSPDGRVIGMDRAP
                     RWLFVTPDSMSETLYKAICSSSK"
BASE COUNT          858 a          862 c          735 g         1024 t
ORIGIN      
        1 cctgatttct ctagtcttaa aatcgagcga ctcggcttgc gaggcgtcaa ccgctccggc
       61 tgcgccgcgc aaaatatcgc cgacggtttt tctatatgcg cgattaggaa aagtcttgtt
      121 tcggcgaatc gacttgcgcg agcctttatc gttttcgccg tagtcgtttc gcctgtctct
      181 ggcgtaactc aaagccttct tttcctgcgg agttcgcttt ctcattaacc gacgcttcct
      241 tccaatttca agcccaaaag tttcgtcgct tcgaccgcgt attccatcgg cagctcgcga
      301 aaaacttcct tgcagaagcc gttgatgatt aatgaaaccg cgtcttcctg cgagatgccg
      361 cgctgctgca aatagaaaag ctgttcttcg ctgattttcg aagtcgtcgc ttcgtgctcg
      421 acgcgcgccg tattgttcat cacctcgata tagggaaaag tgttcgcttc gctttttccg
      481 ccgattagca tcgaatcgca ctgcgtgtaa ttacgagcgc cgctcgcgcg cggcattatt
      541 ttcaccagac cgcgatacga attattcgag cgcccggcgg aaatcccttt ggaaatgatg
      601 gtcgatttcg tgtttttgcc gaggtgaatc atcttcgttc ccgtgtcggc aatctgcgcg
      661 ttgttcgtca gcgcgaccga ataaaattcg ccgatggaat tatcgccctg caaaatcacc
      721 gacggatatt tccacgtaat cgcgcttccg gtttcgacct gcgtccacga aattttcgag
      781 tttttaccgc gacacgcgcc gcgcttggtc acaaaattat aaattccgcc tttgccggat
      841 tcatcgcccg cataccagtt ctgcacggtc gaatatttga tttcggcatc gtccagcgcg
      901 accagctcga cgaccgccgc gtgcagctga ttcgtgtcaa actgcggcgc cgtgcagccc
      961 tcgttgtagg cgacgtaacc gccttcttcg gcgacaatca gcgtgcgctc aaactgtccg
     1021 gattcctgcg tgttgatgcg aaaataggtc gataattcca tcgggcagcg cacgcctttc
     1081 ggaataaaaa cgaacgagcc gtccgaaaaa accgccgaat ttaaagccgc gtaataatta
     1141 tcgctgacgg gaacgaccga gccgagatat ttttttatca gttccgggta atcggcgacc
     1201 gcttcggtaa acgagcagaa aatcacgccc gcttccagca gttttttctt ataggtcgtc
     1261 gccaccgaaa ccgagtcgaa cacggcatcg accgcgacgt ttgccagcat tttctgttcg
     1321 gtcaaaggaa tgccgagctt ttcaaaagtc ttcagcaatt ccgggtcgac ttcgtccaga
     1381 ctctgctttt tcgccttctg cttcggcgcg ctgtaataac tgatattttg aaaatcgatg
     1441 ttcggataat taaaattcgc ccagttatcg ggctgcttca ttttcagcca gtgacgatac
     1501 gctttcaggc gaaattcgag catccattcc ggctcgtttt tcttcgacga aatcaggcga
     1561 accgtatctt cggacaaacc cttggcaatc gtttccgttt cgatgtccgt gacaaaaccg
     1621 tatttatatt ccctgtttgt taataattct gttgctgttg acataaaaat aagttcaatg
     1681 ttgaaagttc aatgttgaaa gtcgcttctc tgcgccaatc gctcgttgcc ctgtcgaccg
     1741 cttaaattct ttgcggctcg atggtgcgct gaatgccctt aatttgatga aacatcgcgt
     1801 cgaccgcgct ttcggttccg accaactgcg ccagcgttat ctgcgacaga gcgttttcga
     1861 caatctcgtg cagcccgacg atcaccggac gaatgccgca atcgcctttg tgaacgcacg
     1921 tctccaaaac gcccgtgtag ctttcgcaat gtttatttaa aacgtcctcg tccagaacct
     1981 tgatgacttc gttcaaacga atctcgctcg ccggacgcgc caggctgtag ccgcctttcg
     2041 tcccgcgcgt cgcctgcaca aaacccgctt tatttaaaag ccacatcagc tttcccgcat
     2101 tcgccactga aattccttcc agctcggcaa tctgcggcaa agtccgcgac tcgttcccgg
     2161 acaaagttgc cagctgcacc agacaacgca atccatattc ttcctgtgcc gatattttca
     2221 tctgtttacc caattaaaaa tttcgcattt tgtagcaaat aatagattat caaatcttta
     2281 cttttttatc aagattgtta gttagggcat aaagtgaaaa tcttagggca taaagtgaaa
     2341 atggcgagtt ttgtaatgga agtattagat agctgacaat tgaaactgct atacataaaa
     2401 ctagtttgtg ctcttctact gttaagtaaa attttggtca gttctagtcc caaaaattaa
     2461 ttgaaataaa acggtcagga taattctcta ccttcacttt taactttaca gggaatttta
     2521 ttcggcaagt cacttttgtc gtcaattaaa acatcaacat aaatatttgc taattgcgca
     2581 tatcgcaaaa ggataggtag aggtggttcg cgttctccag tctcatagtg agaaattgtg
     2641 gagcgatcaa atctatcggc aaatccaagt tgtattagga tttcattttg ggacagtcct
     2701 agctcttctc gaagcttttt aagtttttcg ggcaatcgtt ctgctcggag tcttggtttc
     2761 aagcccatca ggaataaatc ctccttttcc cgaaaggctt gaaataacta tttttacata
     2821 ataattgaca aacagtgcga aatcgcacta cattaagctt gaagtaaaat tttgccgaaa
     2881 ataaggattt agtccatttt tcagcagacg gtttcaacta ggttttgaca ccgccttttt
     2941 ccatttttga gtttgagcaa ttagttattc tctacactgt atttcgcact cttattgtga
     3001 ttgagaatcc tttgctcgat tcggttaaac attataacaa aatagtcatt cagacagata
     3061 ttcaacctga tttttgatta ttagctaagc tcctataaaa gttatgaaaa caattccttc
     3121 tcttttgcta tcaatcacta tcctgctttt cgcttgctgt ttagttttcg ctcaagaaaa
     3181 tcgctggtct tatgtcggga cagatgccag cggcactaga ttttttattg atcggaactc
     3241 aattgaaatt aagggcaaca ggctgcgtgt ctggaataaa ttcttttatg ccaaccagac
     3301 ttatcgcctc agcttgtttg aatgggcttg cgccgagaag aaatatctta tccttgacga
     3361 aacaagctat tctccagacg gaagagttat cggaatggac agagcgccta gatggctgtt
     3421 tgtcacacct gattcaatga gcgaaacttt atataaagca atctgtagct cctctaaac
//
LOCUS       DASWNE010000320         5293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_115934, whole genome shotgun sequence.
ACCESSION   DASWNE010000320 DASWNE010000000
VERSION     DASWNE010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115934"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..3471)
                     /locus_tag="VGB68_06575"
     CDS             complement(85..3471)
                     /locus_tag="VGB68_06575"
                     /inference="COORDINATES: protein motif:HMM:TIGR04519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HEY0048929.1"
                     /translation="MASKDSQTNFAKLRDKILAQNGKQYWRSVEEFVDAPEFEEFVKQ
                     EYPQHAEEWEDGLSRRHFIKIMGASLALAGLSGCVIQPPEKIVPYVRQPEEIVPGKPL
                     FFATAMTLGGVGVGLLAKSNEGRPTKIEGNPEHPGSLGATDVLAQASLLNMYDPDRSQ
                     EVLYRGSSSTWQNFMTQARARIDENRADGGAGIRFLTETVTSPTLIGQMRQLLTELPN
                     ARWYQYEPLNRDNAMAGARMAFGAPVHTIYRFENADRILSLNSDFLSSFNVGYVKNYS
                     KKHNVSEKNLEMNRLYMVETTLTITGAKADHRLALKPSQMSEVAKAIAGALGVAGASG
                     TGLTEHQVNWVNAMARDLQAARGRSIVIAGDNQSPMIHALAHAMNGVLGNVGQTITYT
                     DPFQPNAEISQVDQLRQLIGEIDGGAVKMLVVMGGNPVYTTPADLKLNKERMDKIPLR
                     LHLGQYFDETAELCHWHVSEKNYLEAWSDTRAYDGTVTIIQPLVKPLYDGKSAHELVQ
                     AFFRENFEKNDLDIIREFWQRQGFRTPGTTATTAPTTGGNANTAQPRPQASPATSANT
                     SAGTQPNAQTSASPATTGAVGQTTTTTSQSNTTTSQNGNATGNFEDQWRRAIHNGLIP
                     NTFATTKTVSVNPAFLTQPETKPSGGGSLEISILPDQNVYDGRFTNNGWLQELPRPIT
                     KTTWENIALISPNTARRLGLNQTGRDYNPMTGAGEGTSFINTYGGNLFSDLATLTFQG
                     AEMSKPVPVWIVPGQPDDAVTIFLGYGRTRAGRVGNGLGYNAYEVMRSDAMNYGIGEL
                     KGTGAKTEIASTQTHFSMEGRDILRVYNREVFEAKPDVGHQHDEYEKSMYPRFDYSDR
                     NAPWRHKWGMTIDLNACVGCNACVVACQSENNIPVVGKEQVGRSREMHWLRIDAYYSG
                     DDINNPDGPHFQPVLCQQCEQAPCEVVCPVHATVHSAEGLNDMVYNRCVGTRYCSNNC
                     PYKVRRFNFLLYQDWNTPQYKLMRNPEVTVRSRGVMEKCTYCTQRLALARIEAEKDGR
                     RVRDGEATTACQTVCPADAIVFGDLNDPESHVSKLKHDHRNYVLLNELNTQPRTTYLA
                     ALKNQNPEMPDYIKRDVEETPQEETKDAQTGGEPAKTPGH"
     gene            complement(3565..4221)
                     /locus_tag="VGB68_06580"
     CDS             complement(3565..4221)
                     /locus_tag="VGB68_06580"
                     /inference="COORDINATES: protein motif:HMM:NF014176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEY0048930.1"
                     /translation="MAQVFHRSANNIAKISIVLAVVLGGVAFYVVTQIARSSYVTGRY
                     LEKQQPVQFSHKHHTGDDGIDCRYCHSTVETTASAGMPPTQTCMNCHSQIWSDSPYLE
                     PVRASFRENKPIEWERVHDLPEYVYFNHSIHVNKGVGCSSCHGDVANMPAVYQEYTLQ
                     MEWCLSCHRNVENNVRPKSEIYNTSWVNEQTQAQKDELARQYRLRPSEMLTSCSTCHR
                     "
     gene            complement(4515..>5293)
                     /locus_tag="VGB68_06585"
     CDS             complement(4515..>5293)
                     /locus_tag="VGB68_06585"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY0048931.1"
                     /translation="FINQIKPRFGDFGGRVGVAVSGLIDRRTNRVALSRHTPEYVAVD
                     LAAEVRSATGLQVVLENDANAAAYGEFALGAGRGTHNIFYVLLGTGIGGAFIIEGKLW
                     RGGSGFAGEFGHILVDSEEDLRLEDVANSANILRRIKIRVHQDSTSSLVKIREDELSI
                     ADLVKAANNGDGFTQMMLERTGTYVGTAIASVINLLNIEKIVVGGEVMGAGEPILAGI
                     RRNAQELSFQPSFETTEIVAGQLANRAIATGAALLLNRSE"
BASE COUNT         1042 a         1501 c         1412 g         1338 t
ORIGIN      
        1 caaaatcgtg gtcgccttcg accatcggcg gataaactct ttctctaaat tccttagcgt
       61 cttgcatagt tttacctgtt caacctagtg tccgggcgtc tttgcaggtt ctcctcctgt
      121 ctgcgcatct ttcgtctctt cctgcggcgt ctcctcgaca tcgcgcttga tgtaatcagg
      181 catctcagga ttctgatttt tcaaagcggc taaataagtc gttcgcggct gcgtgttcag
      241 ctcgttcaaa agcacgtagt tgcgatggtc gtgcttgagc ttcgagacgt ggctttcggg
      301 gtcgttcaaa tcgccgaaga cgatagcgtc cgccgggcag accgtctgac aagccgtcgt
      361 cgcttcgccg tcgcggacgc gccgaccgtc tttttccgcc tcgatgcgcg ccagcgccag
      421 gcgctgcgtg cagtaagtgc atttttccat gacgccgcgg ctgcgaaccg tgacttccgg
      481 attgcgcatc aatttgtact gcggcgtgtt ccagtcctgg taaagcagga aattgaagcg
      541 acgcactttg taaggacagt tgttcgagca gtaacgcgtt ccgacgcagc gattgtagac
      601 catatcgttc aagccttcgg cgctgtgcac cgtggcgtga accgggcaga cgacttcgca
      661 cggcgcctgc tcgcactgct ggcagagaac cggctggaaa tgcggtccgt ccgggttgtt
      721 gatgtcgtcg ccgctgtaat aggcgtcgat tctgagccag tgcatctcgc ggcttctgcc
      781 gacctgttcc ttgccgacga ccggaatatt gttttccgat tgacaggcga cgacgcaggc
      841 attgcagccg acgcaggcgt tcaaatcaat cgtcattccc catttgtgac gccacggagc
      901 gtttctgtcc gaataatcga aacgcggata catcgacttt tcatattcgt cgtgctgatg
      961 cccgacgtcc ggtttcgcct cgaacacttc gcggttgtaa acgcgcagaa tgtcgcgtcc
     1021 ttccatactg aaatgcgtct gggtcgaagc aatctcggtt ttcgcgccgg ttcctttcag
     1081 ctcgccgatg ccgtaattca tcgcgtccga gcgcatcact tcatacgcgt tatagccgag
     1141 cccgttgccg acgcgcccgg cgcgcgttcg accgtagcct aagaaaatcg tgaccgcatc
     1201 gtccggctgt cccgggacaa tccagaccgg aacgggtttt gacatctccg cgccctgaaa
     1261 ggtcagcgtc gccaaatcgg aaaatagatt tccgccgtaa gtattgataa agctcgtgcc
     1321 ttctccggcg cccgtcatcg ggttgtagtc cctgccggtt tgattcagtc ccagacgccg
     1381 agcggtgttc gggctgatta gagcgatatt ttcccaggtc gttttggtta tcggtcgcgg
     1441 cagttcctgc agccagccgt tgttggtaaa acgcccgtca taaacgttct ggtcgggcaa
     1501 aatcgaaatt tccagactgc cgccgcccga aggctttgtt tccggctgcg tcaaaaacgc
     1561 cggattgacc gaaaccgttt ttgtcgtcgc aaacgtattg ggaatcaagc cgttatggat
     1621 ggctctgcgc cactggtctt caaaattccc cgtcgcattt ccgttttgcg acgttgtcgt
     1681 atttgattgc gaggtggtcg tcgtagtctg tccgaccgcg ccggtcgtcg cgggcgatgc
     1741 cgatgtctgg gcattcggct gcgtgccggc gctcgtattc gcgctcgtcg ccggagatgc
     1801 ctgcggtcgc ggctgcgccg tatttgcgtt tccgccggtt gtcggcgcgg ttgtcgccgt
     1861 agttccgggc gttctaaaac cttgtctctg ccagaattcc ctgataatgt ccaaatcgtt
     1921 tttttcaaag ttttctctga aaaacgcctg aaccaattcg tgcgcgcttt tgccgtcgta
     1981 gagcggtttg acgagcggct gaataatcgt caccgtgccg tcgtaggcgc gcgtgtcgct
     2041 ccacgcttcg agataatttt tttccgaaac gtgccagtga cacagctcag ccgtttcgtc
     2101 gaaatattga ccgagatgaa gacgaagcgg gattttatcc attcgctcct tatttaattt
     2161 caaatcggcg ggcgtcgtgt aaaccggatt tccgcccatc acgacgagca ttttgaccgc
     2221 gccgccgtcg atttcgccga tcaactggcg cagctggtca acctgtgaaa tctccgcatt
     2281 cggttgaaac gggtcggtgt aggttatggt ctgaccgacg tttccgagca cgccgttcat
     2341 cgcgtgagcc agcgcgtgaa tcatcggcga ttgattatcg cccgcgataa cgattgagcg
     2401 accgcgcgcc gcctgtaaat cgcgcgccat cgcgttgacc cagttgacct gatgctccgt
     2461 cagcccggtg ccgctcgcgc ccgccacgcc cagagcgccg gcaatcgctt tcgcgacctc
     2521 gctcatctgg ctcggcttga gggcaagccg gtggtcggct ttcgcgcccg taatggtcaa
     2581 ggtcgtctcg accatataaa ggcgattcat ctccagattt ttttccgaaa cgttgtgttt
     2641 tttcgagtag tttttgacgt agccgacgtt gaagctggaa agaaaatcgc tgttcagaga
     2701 gagaatccgg tcggcgtttt cgaatcggta aatggtatga accggcgcgc cgaaagccat
     2761 cctcgcgccc gccatcgcgt tatcgcggtt cagcggctcg tattgatacc agcgcgcgtt
     2821 cggcagttcc gtcagcagtt gacgcatctg cccgatgagc gtcggcgaag tgacggtctc
     2881 ggtcaaaaaa cggatgcccg cgccgccgtc cgcgcgattt tcgtcaatcc gcgcgcgcgc
     2941 ctgcgtcata aaattctgcc aggtcgaaga cgagccgcga tacaaaactt cctgcgagcg
     3001 gtcggggtcg tacatattga gcagggacgc ctgcgccaga acgtctgtcg cgccgaggct
     3061 gcccggatgt tccggattgc cttcgatttt ggtcgggcga ccttcattgg atttcgccag
     3121 tagcccgacg ccgacgccgc cgagcgtcat cgcggtggca aaaaacagcg gtttgccggg
     3181 aacgatttct tcgggctggc gaacataagg gacgattttt tcgggcggtt gaataacgca
     3241 gccgcttaag ccggcgagcg ccagagacgc gcccataatc ttgatgaaat gccggcggct
     3301 taaaccgtct tcccattcct cggcgtgctg cggatattcc tgtttgacaa attcttcaaa
     3361 ctcgggcgca tcgacaaatt cctcgacgct ccgccaatat tgttttccgt tttgcgcgag
     3421 aattttatcg cgcaatttcg caaagttagt ttgactgtct ttgcttgcca tgtttttcca
     3481 attcctcagt taaaaaatca aaaactaaaa atgtgcttcg acgcccttca cttttaattt
     3541 ttaattttgc atttttattc aaatctaacg gtgacaagta gaacaactcg tcagcatttc
     3601 cgacgggcgc agacgatatt gccttgccag ctcgtccttt tgcgcctgag tctgctcgtt
     3661 gacccacgac gtattataaa tttccgattt cgggcgcacg ttgttttcca cgttgcggtg
     3721 acacgacaga caccactcca tctgcagagt gtactcctgg taaaccgccg gcatattcgc
     3781 cacgtcgccg tgacacgacg agcagccgac gcctttattg acgtgaatcg agtggttgaa
     3841 atagacgtat tccggaaggt cgtggacgcg ctcccactcg atcggcttgt tttcgcggaa
     3901 actggcgcgc accggctcca gatacgggct gtccgaccag atttggctgt gacagttcat
     3961 acaggtctgc gtcggcggca tcccggcgct cgcggtcgtc tccaccgtcg agtgacagta
     4021 gcggcagtcg atgccgtcgt cgcccgtatg atgtttgtgg ctgaactgga cgggctgctg
     4081 cttttccaga tagcgacccg tgacatatga cgaacgcgct atctgcgtca ccacgtaaaa
     4141 tgcaactccg cctaaaacta ctgccagaac gatactgatt ttggcaatgt tatttgcgct
     4201 tcgatgaaaa acttgagcca tgcttattca ctcgtctctc gattccgaaa agttcgtcgg
     4261 aaaattacag ttcgccctca caaacctgat ttttttgtca agtttttatt aggctggatt
     4321 gtttttttac ttcttctaat actctaccaa aaactttctt taatgttaca gatttttttc
     4381 gccgtctggc aattccactt accaaaataa atgctttcag gactttgtgc aagttttcac
     4441 taaattagtt tgactgataa aactaattac ctttttcgat ttatacggga ctccggaatt
     4501 tatacaaaaa aattttattc gctgcggttc aaaagcagcg ccgcgccggt cgcgatggcg
     4561 cggttggcga gctgcccggc gacgatttcg gtcgtctcga agctcggctg aaacgacaat
     4621 tcctgcgcgt tccgcctgat gcccgccaga atcggctcgc ccgcgcccat cacctcgccg
     4681 ccgaccacga ttttttcgat atttaaaagg ttgatgacgc tggcaatcgc cgttccgaca
     4741 taagttccgg tgcgttcgag catcatctgc gtaaaaccgt cgccgttgtt tgccgccttg
     4801 accaggtcgg cgatgctcaa ttcgtcttcc cggattttga ccagcgaaga cgtgctgtct
     4861 tgatgaaccc tgattttgat gcgccgcaga atatttgccg aattggcgac gtcttcgagc
     4921 cgcaaatctt cttcggaatc gaccagaata tgcccgaatt cgcccgcgaa acccgaaccg
     4981 ccgcgccaga gcttgccttc gatgataaag gcgccgccga tgcccgtgcc gagcagaaca
     5041 tagaaaatat tatgcgttcc gcgccccgcg ccgagcgcga attcgccgta ggcggcggcg
     5101 ttggcgtcgt tttccagaac gacctgaagc ccggtggcgc tgcgaacttc ggcggcaagg
     5161 tcgaccgcga cgtattcggg cgtgtggcgc gacagcgcga cgcgattcgt gcgccggtca
     5221 atcagaccgg aaacggcgac gccgacgcgc ccgccgaaat cgccgaagcg cggctttatt
     5281 tgattaataa aag
//
LOCUS       DASWNE010000321         1731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_151273, whole genome shotgun sequence.
ACCESSION   DASWNE010000321 DASWNE010000000
VERSION     DASWNE010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151273"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VGB68_06590"
     CDS             <1..232
                     /locus_tag="VGB68_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048932.1"
                     /translation="PRESIEKLINIREKAGYLTDAARCQACGYLDEAGISCGHETKSD
                     FKTQIPKTNGTKFSFTREEIIELISQAAQLKQ"
     gene            287..568
                     /gene="eutM"
                     /locus_tag="VGB68_06595"
     CDS             287..568
                     /gene="eutM"
                     /locus_tag="VGB68_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HEY0048933.1"
                     /translation="MQEALGMVETKGLVAMIEAADAMVKAANVQLVGYEKIGAGFVTA
                     IVRGDVAAVKAATDAGAAAARRVGELVSVHVIPRPHGSVDDVMPIGLKG"
     gene            677..943
                     /locus_tag="VGB68_06600"
     CDS             677..943
                     /locus_tag="VGB68_06600"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HEY0048934.1"
                     /translation="MIIARILGTVVSTQKDERLHGKKLLIVKPINLDGTDQSGYLLAV
                     DTVGAGYHEKVIVVAGSSARLAEGNKDCPVDSAIIGVIDTIDFI"
     gene            956..>1731
                     /locus_tag="VGB68_06605"
     CDS             956..>1731
                     /locus_tag="VGB68_06605"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY0048935.1"
                     /translation="MKKSYYTWGFVLTIMLSFCALPAARAQGYGDRNSTGDGKLAIQG
                     KVFLPNGNPAAGVTVSYSGTEFVSGTARTDDSGNFRFNNLPPGNYTVTVRGTEDFETD
                     NETIAMGKESSAGQSFTVVFNLRNKGAKKGDLSANPFFANVPKEALKKYQEAAEGIKK
                     NDLKAAVAALDAAIAIYPDFAIAYNEKGLLLVRQNELDKALEAYVKAIQLKPDYFDAK
                     FNFGYALLNKKEYQQTEMIFRDLLKQKNDMPTAHMYLGIAL"
BASE COUNT          485 a          409 c          484 g          353 t
ORIGIN      
        1 gccgcgcgag tccatcgaaa agctcataaa cattcgcgaa aaagccggat atttaaccga
       61 cgcggcacgc tgccaggctt gcggttatct ggacgaagcg ggcatttcgt gcgggcacga
      121 aacaaaatcg gatttcaaaa cgcagattcc aaaaacaaac gggacgaaat tcagctttac
      181 gcgcgaggag ataatcgagc ttatcagtca agccgcgcag ttgaaacaat aacgagtttc
      241 ggttaaattt agtttccgtc ataataattt caggagcaaa aattgaatgc aagaagcatt
      301 aggaatggtc gagacgaaag gtctcgtagc aatgattgaa gcggctgacg cgatggttaa
      361 agccgctaac gtgcaactgg tcggttacga aaaaatcggc gccggtttcg tcacggcgat
      421 tgtccgcggc gacgtggcgg cggtcaaagc ggcgaccgat gccggagcgg cggcagcgcg
      481 gcgggtcggc gaattggtca gcgttcacgt tatcccgcgc ccgcacggaa gcgtcgacga
      541 cgtgatgccg attggattga aaggctaaag cctttcgtcc atcgtccttt gtcattagtg
      601 cttggcgtct ccgactcaag gaaattattg acattttgcc aaaagcgaag gatgcgggac
      661 gaagaacaaa ggactaatga ttatcgcacg catactcggc acggtcgtct cgacgcagaa
      721 ggacgagcgc ctgcacggca aaaaacttct tatcgttaaa ccgataaatc tcgacggaac
      781 agaccagagc ggttacctgc tcgcggtcga tacggtcggc gccggttacc acgaaaaggt
      841 tatcgtggtc gccggttcgt cggcgcgcct ggcggaaggt aataaagatt gtccggtcga
      901 ttcggcaatc attggtgtaa ttgacacgat tgatttcatt tagagaggta gcagtatgaa
      961 aaagagttat tacacatggg gttttgtatt gacgataatg ctgtcgttct gcgctttgcc
     1021 cgccgcgcgc gcgcagggct acggcgacag aaacagcacc ggcgacggga aactggcgat
     1081 acagggaaaa gtttttttgc cgaacggcaa tccggcggcg ggcgtgacgg tttcctacag
     1141 cggaaccgaa ttcgtcagcg gcacggcgcg aacggacgac agcggcaatt tcagattcaa
     1201 caatctgccg ccgggcaact acacggtgac ggtcagagga accgaggatt tcgagaccga
     1261 caacgaaaca atcgccatgg gcaaggaaag ctcggcggga caatcgttta cagtcgtttt
     1321 caatctaaga aacaagggag ctaaaaaagg cgatttatcg gcgaatcctt ttttcgccaa
     1381 cgtgccgaaa gaagctctga aaaaatacca ggaagcggcg gaaggaatta agaaaaacga
     1441 tctgaaagcg gcagtcgccg ccttggacgc ggcgattgcg atttatcccg attttgcgat
     1501 tgcctacaat gaaaaaggac ttctgctggt cagacaaaac gaattggaca aagcgctcga
     1561 agcatacgtc aaggcaatcc agctcaagcc cgattatttt gatgcgaaat ttaatttcgg
     1621 ctacgcgctg ctcaacaaaa aggaatacca gcaaaccgag atgattttcc gcgatttgct
     1681 gaaacaaaaa aacgatatgc cgacggcgca tatgtatctc ggcatcgcgc t
//
LOCUS       DASWNE010000322         2568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_680258, whole genome shotgun sequence.
ACCESSION   DASWNE010000322 DASWNE010000000
VERSION     DASWNE010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680258"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VGB68_06610"
     CDS             complement(<1..303)
                     /locus_tag="VGB68_06610"
                     /inference="COORDINATES: protein motif:HMM:NF017046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HEY0048936.1"
                     /translation="MKKNIIALISLFAFSVTAMPLGLRAETRDFVPPRTIRITPPAPK
                     FTDAERQSELARRRAEVAKKMAANSLLMMRSAEPRNYAGDVDFYYRQENNLYYLTGL"
     gene            complement(429..2444)
                     /locus_tag="VGB68_06615"
     CDS             complement(429..2444)
                     /locus_tag="VGB68_06615"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048937.1"
                     /translation="MNSKLFFCLFLLILISIFFASCREQEKARKAWTRAASFAGLSRE
                     FGEPFGLAFDAGGVLYVSDGEKGAIFRVSADGKTEIVTEKLNTPSHIAFDKDGFLIVA
                     DAGSHSIRRVDVKNGNVETIAGIDGKKGFQDGEASAALFNAPIGVAVFGDKIFVADTY
                     NDKIRVVENNRVSTLAGNAQGFADGLANQAKFDTPCGIAVSRTGNLIVADTGNKRIRK
                     IDAGGNVTTIAGTGEPDSLDGLPPQAKFVEPLAVAIDEFGVIYVADGNSIRAVNRRFF
                     PLVETISDTKRGFSDGQLRQSRFNRPSGLAVDKNGNLFAADAENQTIRVFTGENLGRE
                     AGSGEIEKLRYTAAEFRAIQTPRWTYNPPAARREIAGTIGEIRGEIAGEKRAWFHNGL
                     DIAGAYGETARFVRTEKVLQPTAADNFGSLRELLRMPTVGYVHIRLGRDASEKIFDDE
                     RFQFSRDASGKINNVRVARGARFEAGEAIGTLNSFNHVHLIAGRSGAEMNALDALELP
                     NLADNIAPVIEKISLTDENWRPFETRNANQRIKLNGKTRIVVRSFDQMDGGAARRRLG
                     VYRLGYQILRAADETPAIEEKTTISFARMPDEDFVSLVYAPGSQSGYTPVTIFDYVVS
                     NEVSGEAARENFLDAANLEAGNYILRVFAADFFGNKTVRDLNIEIIK"
BASE COUNT          575 a          701 c          651 g          641 t
ORIGIN      
        1 taaaccggtc aggtaataaa gattattttc ctgccggtaa taaaagtcca cgtcgcccgc
       61 gtagttgcgc ggctcggcgc tgcgcatcat cagaaggctg ttcgccgcca tttttttcgc
      121 gacttccgcg cggcggcggg caagctccga ctggcgttcc gcgtcggtaa atttcggcgc
      181 gggcggcgtt atgcgaatcg ttctcggcgg aacaaagtcg cgcgtttccg cccgcaagcc
      241 gagcggcatc gccgttaccg aaaaagcgaa taaagaaata agtgcaatga tatttttctt
      301 cataatttct ccaaaaataa attaacagga tggagtaggc ttcgcgactt gataggcagg
      361 ataaaaaatc aaaaagcaac gatttgaatt tttcttatcc tgttcatcct gtctatcctg
      421 tttgaatctt acttaatgat ttcgatattt aaatctctga cggttttgtt tccgaaaaaa
      481 tccgccgcga aaacgcgcag aatataattt cccgcttcca gatttgccgc gtcgagaaaa
      541 ttttcgcgcg ccgcttcgcc gctgacttca ttcgacacga cgtaatcaaa aatcgtcacg
      601 ggcgtgtaac cggactggct gccgggcgcg taaaccagcg acacaaaatc ttcgtccggc
      661 atccgcgcaa atgaaattgt cgttttctct tcaatcgcgg gcgtttcgtc tgcggcgcgc
      721 aggatttgat agcccagacg gtaaacgccc agcctgcgcc gggctgcgcc gccgtccatc
      781 tggtcgaaac tgcgaacgac gatgcgcgtt tttccgttga gcttaatacg ctgatttgca
      841 ttccgggttt caaagggtcg ccaattttcg tccgtcagcg aaattttttc gatgacggga
      901 gcgatattgt cagccagatt cggcaattcg agcgcgtcga gcgcgttcat ttccgcgccg
      961 cttctgcccg caatcaggtg aacgtggttg aaagaattta aagtcccgat tgcctcgccc
     1021 gcttcgaagc gcgcgccgcg cgcgacgcga acgttgttta ttttcccgct cgcgtcgcgg
     1081 ctgaattgaa atctttcgtc gtcgaaaatc ttttcggaag cgtcgcgccc gagccggatg
     1141 tgaacgtagc cgacggtcgg cattcgcaaa agctcgcgca ggctgccgaa attatccgcc
     1201 gccgtcggct gtaaaacttt ttccgtgcgg acgaagcgcg ccgtttcgcc gtaagcgccc
     1261 gcgatgtcca agccgttatg aaaccaggcg cgtttttcgc ccgctatctc gccgcgaatc
     1321 tcgccgatcg tgccggcaat ttcgcgccgc gccgcgggcg gattatacgt ccagcgcggc
     1381 gtttgaatgg cgcgaaattc agccgccgtg tagcgcagtt tttcgatttc cccggaaccg
     1441 gcttccctgc cgagattttc gcccgtaaaa actctgattg tttgattttc cgcgtcggcg
     1501 gcaaaaagat tcccgttttt atcaaccgcc aagcccgacg ggcgattaaa tctcgattgg
     1561 cgcaattgcc cgtcggaaaa tccgcgcttg gtgtctgaaa tcgtttcgac cagcggaaaa
     1621 aatcttctgt taacagcgcg aatcgagttt ccgtcggcga cgtaaatcac gccgaattcg
     1681 tcgattgcga ccgctaaagg ctcgacaaat tttgcctgcg gcggcagccc gtcaagcgaa
     1741 tccggctcgc ccgttccggc tatggtcgtg acgtttccgc ccgcgtcgat ttttcttatt
     1801 ctcttatttc ccgtgtcggc gacgattaaa tttccggttc gtgaaaccgc gattccgcac
     1861 ggcgtgtcga attttgcctg attcgccaga ccgtcggcga aaccctgggc gtttccggcg
     1921 agagtggaaa cccggttgtt ttcgaccacg cggattttgt cgttatacgt atcggcgacg
     1981 aaaattttgt cgccgaaaac ggcgacgccg attggcgcgt taaacaacgc tgcgctcgct
     2041 tcgccgtcct gaaagccttt tttgccgtcg atgcccgcaa tcgtctcgac atttccgttt
     2101 ttgacatcga cgcgcctgat tgaatggctg cccgcatcgg cgacaatcaa aaagccgtct
     2161 ttgtcgaaag cgatgtgcga aggcgtgttg agcttttcgg taacgatttc cgttttgccg
     2221 tccgccgaaa cgcgaaaaat cgcgcctttt tcgccgtccg agacgtataa aacgccgccc
     2281 gcgtcaaatg ccagcccgaa aggctcgccg aattcgcggc ttaatccggc aaaggaagcg
     2341 gcgcgcgtcc acgccttccg cgccttttcc tgctcgcggc aggaagcaaa aaaaattgaa
     2401 attaaaatta aaagaaaaag acaaaaaaat agcttcgaat tcattaatgc catcgtagat
     2461 tctaaaaatt taagatgcaa atcctatgcg gtcggtgaaa ttttgttacc attttaatat
     2521 tccacaaatt ttcaaggagg aaaaaagaat atgttttcat tacaggat
//
LOCUS       DASWNE010000323         2120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_293330, whole genome shotgun sequence.
ACCESSION   DASWNE010000323 DASWNE010000000
VERSION     DASWNE010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293330"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            616..>2120
                     /locus_tag="VGB68_06620"
     CDS             616..>2120
                     /locus_tag="VGB68_06620"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0048938.1"
                     /translation="MANKALVIDDDVATLELMRFQLEAEGFEVSTAEHAQQGLDLIKE
                     IEFDIILTDLNLPDISGIEMVKRAKETLPETEIIMVTGFGSTEKAIEATKAGAFYYVE
                     KPIEFEELIVLIEKAIDRKRSAAEIRELRGKLSNRNSYEGIIGASKAMQDIYEMIDGV
                     AESDANILILGESGTGKEIIANAIHYKSTRAKKSFIKVNCSALPKELIESELFGHTRG
                     AFTGATQDKIGYIGRANGGSLLLDEIGEMPIDLQPKLLRVLQDKVYYRVGSDKSQEVD
                     FRLISATNRNPFDAIQDGSLREDLYYRINTIEIKIPPLRERMEDVPMLAEHFLIMYAE
                     KYNRNVTSFSQKAFGQMLSYNWRGNVRELQHVVERAILLSKGSTVQELNIPTSAEMNP
                     SAVMSAPGNAVRQVSTPAFSDSPAMSAAEIVSGSSHFSTNNLFNFSDEQLFEEIGKFI
                     VNKLPEPVDGQDQKDVFDSIEYGVVLAALKRTNGNKQAAANLLGLYRPRLYG"
BASE COUNT          605 a          453 c          503 g          559 t
ORIGIN      
        1 ttatgattgc ttgttgaaac gacggcttca ttcacactaa gagcgctttt tttgacgatt
       61 cgaaccggag cttttttcat tatttcttac ctgaattgat ttgtatctat ataaagactc
      121 tgccagataa aagcaatccg aatgccagaa gttaaagcca gtaaagacgc gatttttaaa
      181 ataaaatcaa aaaccgattt tcgtgtttgt atgaaaccga tacatcgggc tggattaatt
      241 aaatacatta ttttagtatc aattacaaca gatttatgcg gaatctcgga atatgcttag
      301 ccgcgtctgt gtggcataat cattgctcag gcaaacggta tcaaaactta ctgtttttcg
      361 cagaaatcaa gaattaatca agaattaata cggtatcgga aattaaataa aacaatttaa
      421 ggtagtatac tgtaaagaac gaatcgtttt tggaaaacaa atttgatgaa tcggaaaata
      481 aagttgaatt aaacacaagt taaaacacat caaaagcaaa cattttgctt ttttttgccg
      541 ttttgatttt ccttccctgt gctttttttc gtattatttt attacaataa ggttgtattt
      601 tgacgaggct tttctatggc aaacaaagct ctggtaattg atgacgatgt ggcaactctg
      661 gagttgatgc gatttcagct tgaagctgaa ggttttgaag tatcgaccgc ggagcacgcc
      721 cagcagggtt tggatttaat caaagagatt gaattcgaca ttattctcac cgatttgaat
      781 ctacccgaca tcagcgggat agaaatggtg aagcgcgcga aggaaactct gccggagacc
      841 gagattataa tggtcaccgg tttcggctcg accgaaaagg cgattgaggc gaccaaagcc
      901 ggcgcttttt attacgtcga gaagccgatc gaatttgaag aactcatcgt ccttatcgaa
      961 aaggcgattg accgcaagcg ctcggcggcg gaaatccgcg aattgcgcgg aaagctttcc
     1021 aatcgaaatt cctacgaagg catcatcggc gcgagcaagg cgatgcagga tatttacgag
     1081 atgattgacg gcgtcgccga atcggacgcg aatattctta ttctcggcga gtcgggaacc
     1141 ggaaaggaaa ttatcgccaa cgcgattcat tacaagagca cgcgcgccaa aaaatctttt
     1201 attaaagtca attgttccgc tttgccgaaa gaattgattg aatccgagct tttcgggcat
     1261 acaagaggcg cgtttaccgg cgcgacacag gacaaaatcg gttatatcgg acgagcgaac
     1321 ggcggcagcc tgctgctcga cgaaatcggc gagatgccga ttgatttaca gccgaagctg
     1381 cttcgcgtct tacaggataa ggtttattac cgcgtcggca gcgacaaatc gcaggaagtc
     1441 gattttcgtt tgatttcggc aactaatcgt aacccgttcg acgcgattca ggacggctca
     1501 ctgcgcgaag acctttatta ccgcatcaac accatcgaga taaaaattcc gccgctgcgc
     1561 gagagaatgg aagacgttcc gatgctggct gagcattttc tgataatgta tgcggaaaaa
     1621 tataaccgaa acgtaacgag tttttcgcag aaggctttcg gtcagatgct ttcatacaac
     1681 tggcgcggaa acgtgcgcga gcttcaacac gtcgtcgagc gcgcgattct gctcagcaaa
     1741 ggcagcaccg tgcaggagtt gaacattccg acttccgccg agatgaatcc gtcagccgtt
     1801 atgtccgcgc cgggaaatgc cgtccgacag gtctcgacgc cggctttttc agattcgccg
     1861 gcgatgtccg cagcggaaat cgtttcgggc agctctcatt tctcgacaaa caatttattt
     1921 aacttttcgg atgaacagct ttttgaagaa atcggaaaat ttattgttaa taaattgccc
     1981 gagccggttg acgggcaaga ccagaaagac gttttcgatt cgatagaata cggcgtcgtg
     2041 cttgccgctc tcaagcgaac caatggcaac aaacaggcgg cggcgaatct tttaggtttg
     2101 tatcgaccgc gtctttacgg
//
LOCUS       DASWNE010000324         2607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_362465, whole genome shotgun sequence.
ACCESSION   DASWNE010000324 DASWNE010000000
VERSION     DASWNE010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362465"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VGB68_06625"
     CDS             complement(<1..765)
                     /locus_tag="VGB68_06625"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEY0048939.1"
                     /translation="MSAVTTNKPVTKSKTNGRAATAANGRAKTNGHAKTDFLDQKLLL
                     SVLTAVKKGDFSSRMPTDEIGIAGKIYDALNEIIERNEMLTSELGRISEVVGKEGKIA
                     QRATLSHATGAWASCISSVNTLITDLAQPTTEVARVIGAVAEGDLSQNFNLEIDGRPL
                     KGEFLRTGKTVNTMVAQLGAFASEVTRVAREVGTEGKLGGQAQVKGVSGTWKDLTDNV
                     NLMASNLTGQVRNIADVTTAVANGDLGKKITVDVKGE"
     gene            1177..1566
                     /locus_tag="VGB68_06630"
     CDS             1177..1566
                     /locus_tag="VGB68_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0048940.1"
                     /translation="MSYFEVQAMPKTILIVEDYADSRSLMRFMLESNGYAVLEASNGE
                     EAVESVKSQVPDLILMDMSMPVMDGLTATRILRKFDATARLPIIAVTAYGKSYYRQAM
                     EAGCDDLINKPLDPRVLEPILDQYLGH"
     gene            1807..2490
                     /locus_tag="VGB68_06635"
     CDS             1807..2490
                     /locus_tag="VGB68_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048941.1"
                     /translation="MLLIIARISLILFLVAVPFLFYGFGRETLGLIPQISPANPRWFA
                     FLVGAAAFIPSLLVAKRLFPATWSYLQTLEHELTHLLVGLLFLKIPVGIRVSAHEGGE
                     VRQIGLGTTGQTWVTLAPYFFPTVSLAVLIFAYFARLNSGLLLGILGWTTVFHLVTNW
                     SETSFRQPDLQKAGLLKTLLILPVMNLICYGSVLAFAAGGGKGFGAFWYQGLSASLGL
                     LKQVIFRIF"
BASE COUNT          553 a          663 c          658 g          733 t
ORIGIN      
        1 ttcgcctttt acgtcgacgg taatcttttt acccaaatcg ccgttcgcca cggcggtcgt
       61 cacgtcggcg atgttgcgca cctgtccggt caggttcgac gccatcaggt ttacgttgtc
      121 ggtcaggtct ttccacgttc ccgaaacgcc tttgacctgc gcctgaccgc caagctttcc
      181 ttcggttccc acttcacgag ccacgcgcgt tacttcggat gcgaaagcgc cgagctgcgc
      241 gaccatcgtg ttgacggttt tacccgtgcg caggaattcg cctttgagcg gtcgcccgtc
      301 gatttccaga ttgaaattct gcgacaaatc gccttccgcc accgcgccga taacgcgggc
      361 gacttccgtc gtcggctgtg ccaggtcggt aatcaaagtg ttgaccgagc tgatacacga
      421 agcccacgct cccgtagcgt gactcagcgt tgcgcgctgc gcgatttttc cttccttacc
      481 gacgacttcg ctgatgcgcc cgagctcgga cgtgagcatc tcgttgcgct cgataatttc
      541 attcagagca tcatagattt tgcccgcaat cccgatttcg tcagtcggca tacggctcga
      601 gaaatcgcct tttttgactg ccgtcaggac gcttaataat aatttctggt cgagaaaatc
      661 ggtcttggcg tgcccgttgg ttttggcacg cccgttggcg gcggttgcgg cgcgcccgtt
      721 ggtttttgat ttggtgacag gcttattggt cgtcacagcg ctcatatttt ggtccccttt
      781 tccgaacaag tataagaatt aatggaattg tagttttttt gactcgcaga atagatagtt
      841 tagctgcaaa aagactaact ctccaattag tataaaactt catcaaatct atttctagtg
      901 aaatatttcc tgagcgctaa aggcgaagct ccaagcggca agctcgtaac tttctaattg
      961 cagcccggcg ctcctgcctt tgccgctttt cgctcaatca gagtttttgt taagccaaat
     1021 tttttattgt aatctggaaa caaaaggagt aatttaaata gtactccggt ttagcgaaac
     1081 tgccggattc aaacttgtta gctttataag cgggaaattt caaacgggga atttcttaat
     1141 gaacgctctg gtattttaag ggagaggatt aattgaatgt cctattttga agtacaagct
     1201 atgcccaaaa cgattcttat tgtcgaagat tatgccgatt cccgcagcct gatgcggttt
     1261 atgctggaat cgaacggtta cgccgtgctg gaagcttcga acggcgagga ggcggtcgaa
     1321 tcggttaaat cgcaggttcc ggatttaatc ctgatggata tgtcgatgcc cgtgatggac
     1381 ggtttgacgg cgacgcgcat tctcagaaaa ttcgacgcga ccgcccgtct gccgattatc
     1441 gccgtcaccg cttatggaaa atcctattac cggcaggcga tggaagcggg ctgcgacgat
     1501 ttaatcaaca agccgctcga cccgcgcgtg ctcgagccta ttctcgacca atatctcggt
     1561 cattaggctc ggctggagtt ttgaatttca gaatcaaata aaaccgtcgt ttcagaattg
     1621 ccgggacaaa gctcgtattt gtcccggcaa ttttttgccg aaaatttcgc cgctttccga
     1681 accggacatc tcgaaaaatg aaaatcttcc cggaacatag cctcgacgtt aaccgtttag
     1741 gattggtgag ctaatcggga gcggataaag tttactaatc gggagcggat aaagtttctc
     1801 gtaaaaatgt tattgattat cgccagaata tcgttgatat tgtttctcgt cgccgtgccg
     1861 tttctgtttt acggcttcgg cagggaaacg ctcgggctga ttccgcaaat ctcgcccgcc
     1921 aatccgcgct ggtttgcctt tctggtcggc gcagccgctt ttattcccag cctgctcgtc
     1981 gccaaacgtc tttttccggc gacctggtcg tatctgcaaa cgctcgaaca cgagctgacg
     2041 catttgctgg tcggcttgct gtttctgaaa attccggtcg gcattcgcgt cagcgcgcac
     2101 gaaggcggcg aagtccggca aatcgggctc ggcacgaccg ggcagacgtg ggtcacgctg
     2161 gcgccgtatt tttttccgac cgtttcgctg gcggttttga tttttgctta tttcgcccgt
     2221 ctaaactccg ggcttctgct cggaatctta ggctggacga ccgtttttca tctggtcacc
     2281 aactggagcg aaacgagctt tcgccagccc gatttgcaga aagccggttt attgaaaacg
     2341 cttctgattc tgccggtgat gaacctgatt tgctacggct cggtgctggc tttcgcggcg
     2401 ggcggcggaa aaggcttcgg cgctttctgg tatcagggct taagcgcgtc gctcggttta
     2461 ttgaaacagg taatttttcg catcttttaa cgaaacgaat ttttcgcggg cagggctttg
     2521 tttgcgcctc cggcttgtat attatattta tccaaataat taaggagccg ctttcgatgc
     2581 cgaaaaatac aaatattctt ttgattc
//
LOCUS       DASWNE010000325         2295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_434664, whole genome shotgun sequence.
ACCESSION   DASWNE010000325 DASWNE010000000
VERSION     DASWNE010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434664"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VGB68_06640"
     CDS             <1..216
                     /locus_tag="VGB68_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048942.1"
                     /translation="KGLLYRELDPRRNPGSTVMFQSVAGGEPKPFLSVKSDSVLNISQ
                     SKDGSRMLVVRDKFVTDAVMLTQIQPN"
     gene            complement(317..1777)
                     /locus_tag="VGB68_06645"
     CDS             complement(317..1777)
                     /locus_tag="VGB68_06645"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0048943.1"
                     /translation="MKDAYDLIVIGGGSAGLVAAGGAGVLGARVALVEKNLLGGDCLY
                     TGCVPSKTLIKSARFAHQLKRAGDFGFEISDFKFQNDSFASVTDRVRKIIEIIEQHDA
                     PEVFEKMGVEIVFGAPRFINSNEIEVALKGSNETRKLKARRFCVATGSRPVVPPIEGL
                     KEAGFITNEEVFGLKKLPERLIVLGGGAIGAELGQSFRRFGSRVTIVEMSQRILIKED
                     EEASRLMTQILKNEGVEILTSTKAVRAKKSVAGAKILIVEKDGAESEIEADEILVAVG
                     RQPNTDALELEKAGVKFDEKQIFTDEYLQTSQKHIFAAGDVTAHFQFTHMADYEAQIV
                     VRNAFVPFPFKKKTDFRVVPWATFTEPEVGRVGLTETEAREKFGDKVKVYKISLTEND
                     RAQAESETEGFTKVVAHKNKIVGAHLVGAHAGEIIHEFVWAMRENLKIPELNKIIRVY
                     PTLAKSAQAVGTQATLETLKSPFVRKWFARYLKIWR"
     gene            1919..>2295
                     /locus_tag="VGB68_06650"
     CDS             1919..>2295
                     /locus_tag="VGB68_06650"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0048944.1"
                     /translation="MSELQKQLRQTKPFKSKEEELYLNLARTTEALNWKVAELLKSHE
                     LTGVQYNVLRILRGAGEEGLICSEISERLITKDSDITRLLERMENRELISRRRSAKDR
                     RHVITKITDTGLKLLAELDEPVV"
BASE COUNT          567 a          587 c          544 g          597 t
ORIGIN      
        1 aaaggtctgc tttaccggga attggacccg aggcgcaacc caggctcgac cgttatgttt
       61 caatccgtcg cgggcggcga accgaaaccg tttttatcgg ttaaatccga ttcggttctg
      121 aacatctcgc aatcgaagga cggcagccgg atgctggtcg tgcgcgacaa attcgtgacc
      181 gacgccgtaa tgctgacgca aattcagccg aactaaccgg gaactgatag ctgatagctg
      241 atagctgata actaaaaaca cttctcaatt atcagttatc agttatcaac tatagttatc
      301 aattatcggt taaagcttat cgccaaattt tcagatagcg cgcgaaccat tttcgcacga
      361 acggcgattt taaagtttcg agcgtcgctt gcgtgccgac ggcttgcgcg gatttggcga
      421 gcgtcgggta aacgcggatg attttattta attccgggat tttcaaattc tcgcgcatcg
      481 cccagacaaa ttcgtgaata atctcgcccg cgtgcgcgcc gaccagatgc gcgccgacga
      541 ttttgttttt gtgcgcgacg actttcgtaa aaccttcggt ttccgattca gcctgagcgc
      601 ggtcgttctc cgtcaacgaa attttataaa cttttacctt atcgccgaat ttttctctgg
      661 cttccgtttc ggtcaaaccg actctgccga cttccggttc ggtaaaagtc gcccacggca
      721 cgacgcggaa atcggttttc tttttaaaag gaaacggcac aaatgcattc cggacaacga
      781 tttgcgcctc gtaatccgcc atatgcgtaa attgaaaatg cgccgtcacg tcgcccgccg
      841 caaaaatgtg tttttgcgaa gtttgcagat actcgtcggt gaaaatttgt ttttcgtcga
      901 atttgacgcc cgctttttca agctctaaag cgtccgtgtt cggctggcga ccgacggcga
      961 ccagaatttc gtcggcttcg atttccgatt ccgcgccgtc tttctcgaca atcagaatct
     1021 tcgcgcccgc gacgcttttt ttcgcccgga cggcttttgt actcgtcaga atttcgacgc
     1081 cttcattttt taaaatttgc gtcatcaaac gggaagcttc ttcgtcttct tttatcaaaa
     1141 tgcgttgcga catctcgaca atcgtgacgc ggctgccgaa tctccggaaa gattgtccga
     1201 gctccgcgcc aatcgcgccg ccgccgagaa cgattaatct ttccggcaat tttttgagcc
     1261 cgaagacttc ttcattcgta ataaaaccgg cttcttttaa gccttcaatc ggcggaacga
     1321 cgggacgcga gccggtggcg acgcaaaaac gcctggcttt cagctttctc gtttcgtttg
     1381 aaccttttaa agccacttcg atttcattcg aattaataaa tcgcggcgcg ccgaaaacga
     1441 tttccacgcc cattttttca aaaacttccg gcgcgtcgtg ctgctcgatg atttcgataa
     1501 ttttccgcac gcggtcggta acggaagcga aagaatcgtt ttgaaatttg aaatccgaaa
     1561 tctcgaagcc gaaatctccg gcgcgtttta attgatgcgc aaagcgcgcc gatttaatca
     1621 gggttttcga aggtacgcag cccgtgtaaa gacaatcgcc gccgagcaga tttttttcga
     1681 cgagcgcgac ccgcgcgccc aaaacgcccg cgccgcccgc cgccacgagt cccgccgaac
     1741 cgccgccgat cacgataagg tcatacgcat ctttcataag atgaaagcag agtagcctgt
     1801 ttttaaacga aaatctacga atttattttt tgtttttttt gaccgcggcg gcggtttatt
     1861 tccttttaat aaaaaatcat ttgacaatag tcgttataac gactattctt tttatgttat
     1921 gagtgaattg caaaaacagc tcaggcagac gaagccgttt aaaagcaagg aagaagagct
     1981 ttatttaaat ctggcgcgca cgacggaagc tttaaactgg aaagtggcag aactgctgaa
     2041 aagccatgaa ttgaccggcg ttcagtacaa tgtgctgaga attttgcgcg gcgcgggcga
     2101 agaaggcttg atttgcagcg aaatcagcga gcgcctgatt acaaaagact ctgacattac
     2161 gagactgctc gaaagaatgg aaaaccgcga gctaatctcg cgccgacgaa gcgcgaaaga
     2221 ccgccgccac gtgattacga aaatcaccga tacgggctta aagcttttag ccgagcttga
     2281 cgagccggtc gtcag
//
LOCUS       DASWNE010000326        10960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_362479, whole genome shotgun sequence.
ACCESSION   DASWNE010000326 DASWNE010000000
VERSION     DASWNE010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10960
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362479"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..411)
                     /locus_tag="VGB68_06655"
     CDS             complement(31..411)
                     /locus_tag="VGB68_06655"
                     /inference="COORDINATES: protein motif:HMM:NF017355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEY0048945.1"
                     /translation="MNKTTKKQVKKTKKESLPKVEQYLYSVGWSEEDEAFVARVAEFP
                     SLAAHGEMQEEALREIKTVVEFVLKDLTKSNEPIPEPFGKRSFSGKLVLRMPEYMHRQ
                     LALEAMQQGISLNQLLNLKLEASR"
     gene            complement(404..682)
                     /locus_tag="VGB68_06660"
     CDS             complement(404..682)
                     /locus_tag="VGB68_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048946.1"
                     /translation="MQKQIELLERVIKEIADNEKQIRFSRILSICEDVFGEYRVSGSH
                     HIFKTPWQGDPRINLQKGKGNNAKPYQVRQVLRALRKLKELEEDKKDE"
     gene            830..1687
                     /locus_tag="VGB68_06665"
     CDS             830..1687
                     /locus_tag="VGB68_06665"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEY0048947.1"
                     /translation="MSYEKAKEAADFIRSRYAGEIKVAIVLGSGLGAFAEDLENAVRI
                     SYEEIPNFQKSTVEGHAGQLVLGEIEGVSVAVQQGRFHYYEGYDMQQVILPVRTFGLL
                     GIKKLILTNAAGSVRTSMKPGGLMLIRDHINLMGTNPLRGANDSRFGVRFPDMTNVYD
                     IEYQEIASAEAKKMSLERVEKGIDREARQLLRRGVYCGLSGPCYETPAEIRMIRLLGA
                     DAVGMSTVPEAIAARHQEIKVLGISCITNLAAGMSDDIIHHEEVMETGARVATVFKAL
                     LRRIIPKLN"
     gene            1770..2399
                     /gene="udk"
                     /locus_tag="VGB68_06670"
     CDS             1770..2399
                     /gene="udk"
                     /locus_tag="VGB68_06670"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEY0048948.1"
                     /translation="MIIGICGGTGSGKTTIARKILDAVGRGNVVLVEQDSYYRNLGDM
                     PLDERRQANFDHPDSIDSDMIVNHLKRLKNGQPIEMPIYDFVTHARKDETEHIDPKPV
                     VILEGILIFAESRVLDLLDVRVFVDTPSDIRLMRRLRRDIEQRGRSFEQTLWQYEHTI
                     RPMHYEFVEPSKRHADVIIPEGSQTEISVELLCGLVREKLRREENLAAK"
     gene            2506..2904
                     /locus_tag="VGB68_06675"
     CDS             2506..2904
                     /locus_tag="VGB68_06675"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015880869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEY0048949.1"
                     /translation="MNEDLRKLVEAARDVRERAYAPYSEFRVGAAIRTKGGKIYTGCN
                     VESASYGLTVCAERVAIWKAVSEGEKEFTDIAVVADTQELTPPCGVCRQIIWEFCNDI
                     PVTFANLHGKTETVQMKELLPRAFDTKFLK"
     gene            3100..4530
                     /locus_tag="VGB68_06680"
     CDS             3100..4530
                     /locus_tag="VGB68_06680"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY0048950.1"
                     /translation="MEKSKILALVFLLILIILLPSVFAQRQSLPRKIVPNRNADLLIV
                     GGTVVTMDKDRRVIEDGAVVVKNGEIALVGKRAAVTKNLRARQVINANGKVIIPGLIN
                     THTHIPMVLFRGISDDLDLNEWLTKYIFPAEAKNVTEDFVRAGTRLGLAEMIRGGTTT
                     YCDMYYFEDAIADETAKAGVRGVLGETIIQFPVADNKTPEDAMRYSERYVNKWKNHDL
                     IVPALAPHAPYTLTTDHLRAIKNLSDKLGAPVVIHVAETRREVEDISKQYGARPVEYL
                     NRIGFLNDRTIAAHVVFANDNEIQILKSRGVGVAHNPQSNMKLASGVSPVPQMLNADI
                     RLGLGTDGAASNNDLNLWEEMDTAAKLHKLYANDPKVLNAEQAFMMATVGGARALHLE
                     NLIGSIEAGKRADIVLVDFDGLHQTPFYNVYSHLVYATKAADVDTVIINGKIIMREKR
                     LLTLDENAIKKDANLYRQKIIKSLSN"
     gene            4753..6018
                     /locus_tag="VGB68_06685"
     CDS             4753..6018
                     /locus_tag="VGB68_06685"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY0048951.1"
                     /translation="MRGRVTKAKASIAVKNYNAAIYELEGIRREINDPTVHSVVNVML
                     MNCYLEQGDYKRAQGFLNELFNAQKANKPNAAQNYFAVAAQVVKGARNQQERYKSLGL
                     MMNDRNLTPDAIADIEKMRETVELVITQTKAAGTDKKKASDAFALLEESSNARIALAR
                     DDYDAKRWKEAVADAREGLMSSRSTVLNAAGDDEVNNGTVASNQPTYTNSTPNTSPKT
                     ETQPSLIQTSNQIAPKTETKTENTTEASNNVKLEPVPERKQTVTTPEKTVAETPNTTD
                     APKETVAEKQLTPNVNPDNEPKVPTRERRVNTPNISTETVAQNTNQTPKAGDDTPLQV
                     GSLVEYATQKVNPVYPPQAKTLRMTGTVKVEVMVDEQGQVSAVQNSSGPSLLQRAATD
                     ALKKWKFKPFTKDGQPVKAKGFVNFNFNL"
     gene            6127..8943
                     /locus_tag="VGB68_06690"
     CDS             6127..8943
                     /locus_tag="VGB68_06690"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEY0048952.1"
                     /translation="MLLRLLFFVFLLTMTVYPQIPPVETGVSQTLARWRAANYSDVRY
                     RLNIKLEKQAPLMKGELEIRVNLTEEGAKNDLILDWRTTQFQNDKEKPYAKVVGVNNK
                     TVSLIGGVDMPNYYVFNEHLGVSRFLLKTGENVIKIEFASPIKTSGAAVTRYVDKEDG
                     AEYVYSLFVPSEASTAFPVFDQPDLKARFSLSLTVPRGWRAISNTEVESKFTIDTTHC
                     RQNCDFDGFDFHETKPASTYVFAFAAGDFEVYRGCLYGDNKPEGKNVPNCLETSSRES
                     ETRDWSKIYVRKSQAEKFKPHAAETFRLNREAVKYLETYFDFKFPFPKYDLVLIPEFP
                     FNGMEHAGATFLRESSVIFPSEPTKNDLLSRANVIFHEAAHQWFGDTVTMRWFDDLWL
                     KEGFAEFMAYKTLEKVLPEYNAWKAFYERNKPLAYLTDSTKGTTPIYQEIPNLNSAKS
                     AYGNIVYRKAPSFLKQAEFYLGEREFQTAVRAFLRKHQFSNAGWTDLLSEFNAVTKSA
                     YVRDFSTAWVKQPGLFKIRLNQGDFVNSAQYKFVLNAEAQHSIEQNAERVMKFKILVI
                     NADGSRDIREFMLNPQRSSHRDTFPLSQNPALVFPNYEDYGYGIFLLDERSRAYVLEN
                     IRNEKDDFLRSMMWGTLWDSVREGELNPRDYVELAVKNIAVETDELTIQTILSRVSTA
                     MNYYLSDAQRAESATKLEDLLVEKFTAAKTKGQRLTFYRAFLNIASSKKAQTVLKDIL
                     KANTGKQEPEAPKKNSAKDEKRAEFFELPALSTKDKFDIVTRLAVLGDADASRLLDEL
                     QKKETSDDAKRYAYAVGAGFGTKEAKEKYFNDFLNNKEISESWIESAFVPFNSVRHSE
                     LTLPHLEKALAELPNLKRSRKIFFVNGWLAAFIGGQKDEKALSAVNKFLANNPNLDKD
                     LRLKIMENVDALERAVKIRAKYGK"
     gene            9279..9710
                     /locus_tag="VGB68_06695"
     CDS             9279..9710
                     /locus_tag="VGB68_06695"
                     /inference="COORDINATES: protein motif:HMM:NF025683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HEY0048953.1"
                     /translation="MAKTIATIMGIVFILIGLVGFISDNFMGTHLSMTHNLIHLISGA
                     VALYLGLKGSLSAARLFCLAFGAVYLLLGLAGFLFNSGTTTGLPANLTDGGTNTHMLH
                     VLPGALELGIMDHIVHVLLGLIFLVGGFLTRADVRTDNARV"
     gene            complement(9801..>10960)
                     /locus_tag="VGB68_06700"
     CDS             complement(9801..>10960)
                     /locus_tag="VGB68_06700"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEY0048954.1"
                     /translation="WREDLVLARELGLHALRWGVPWYKVEPESGKFDWSWTDKVIPYL
                     VEDLKITPILDLMHYGTPFWLKKEFANNDYPEYVARYAAEFARRYRGLIKWYTPLNEP
                     IINALMCGMRGLWAPYLKGEKGYIRVMLQLAKGIIRTVNAIKEIDPETVMVHVEATGM
                     TRTVREDLAVLANEETHRGYLCYDLISGRIKHDHLLFSWLVRNGASLDDLDQLQGNKI
                     PLDVVGMNFYPQWSTRQIYLDKRGRLAFRDIEPKGNGFVDLIRDFHERYQVPIMITET
                     SAVGADEVRERWLESSVGMIKNLRADGVPVIGYTWFPLFTMIDWRYRFSQEPLENFYL
                     ELGLYKLNREAAGKRWLETPLVEKYKNYIKSPMEAVGNLTVKQKETAEAYT"
BASE COUNT         2917 a         2666 c         2770 g         2607 t
ORIGIN      
        1 atcggaagga aaaataaaaa ggaagcaagt tcagcggctt gcttccaact tcaaatttaa
       61 taattgatta agcgaaatgc cttgctgcat cgcttcgagc gcaagttggc gatgcatata
      121 ttcgggcatt cgcaaaacca atttgccgct aaaagaacgc ttgccgaaag gctcgggaat
      181 cggctcgttc gatttcgtca aatctttcaa gacaaattcg acgaccgtct taatttccct
      241 caaagcttct tcctgcattt cgccgtgcgc ggctaaagac ggaaactcag ccacgcgagc
      301 gacaaatgct tcgtcttctt cagaccagcc gacgctgtaa agatattgct cgaccttcgg
      361 caaactttcc tttttcgttt ttttgacttg tttcttagtc gttttattca tctttcttat
      421 cttcttcaag ttcttttaac ttacgcaaag cgcgaagaac ctgtcgaacc tgataaggtt
      481 tggcgttatt gccttttcct ttttgcaagt taatgcgcgg gtcgccttgc cagggagttt
      541 taaaaatatg atggcttccg ctcacgcgat actcaccaaa aacatcttca caaatagata
      601 aaatcctaga gaaacgaatt tgcttctcgt tatcagcgat ttcttttatt actctttcaa
      661 gtaattcaat ctgtttttgc atttatttct catcgctgcg tttctgtatt tttgaaaact
      721 gtctgataag gattatttga aagaatgata ctatatcaag tatcacattt caaataaaac
      781 aaatgtaaat ttactcaaac tcagtctttg tgtaaaaatg aaattcttta tgagctacga
      841 gaaagcgaag gaagcggctg attttatccg ctcgagatac gccggggaaa taaaggtggc
      901 gattgttttg ggcagcgggc tgggcgcgtt tgccgaagat ttggagaatg ccgtgaggat
      961 ttcttatgaa gaaattccta attttcagaa atcgacggtc gaaggtcacg ccggacaatt
     1021 ggttctgggc gaaatcgaag gcgtgtcggt tgccgttcag caggggcgtt ttcattatta
     1081 cgaaggctac gatatgcagc aggttatttt gccggtgcgg acgtttggct tgctcggaat
     1141 aaaaaagctg attttgacga atgcggcggg cagcgtgcgc accagtatga agccgggcgg
     1201 tttgatgctg attcgcgacc atattaattt aatgggcacg aatccgctgc gcggcgcaaa
     1261 cgactcgcgt ttcggcgtgc gctttcccga tatgacgaat gtttacgaca tcgaatacca
     1321 ggaaattgcc agcgcggaag cgaaaaagat gtcgctcgaa agagtcgaaa agggaattga
     1381 cagggaagcg cgccagcttt tgcggcgcgg cgtttactgc ggtttatcgg gtccgtgtta
     1441 cgagaccccg gcggagattc ggatgatacg gctgttgggc gcggacgccg tcgggatgtc
     1501 gaccgtgccg gaagcgattg cggcgcggca tcaggaaata aaagttttgg gaatttcctg
     1561 cattacgaat ctcgccgccg gaatgagcga cgacattatt catcacgaag aagtgatgga
     1621 gacgggcgcg cgcgtagcga cagtgtttaa agcgctttta agaagaatta tcccaaagtt
     1681 gaattaaaac aggatggaca agatggacag gattataaaa aaaataactt ctttatttta
     1741 atcctgctta tccggtttga aatcttatta tgattatcgg aatttgcggc ggaaccggct
     1801 caggaaaaac gacgattgca cgaaagattc tcgatgcggt cgggcgcgga aacgtcgtgc
     1861 tggtcgaaca ggactcttat tatcggaatc tcggcgatat gccgctggac gagcggcggc
     1921 aagcgaattt cgaccacccc gattcgattg acagcgatat gattgtcaat catctgaagc
     1981 gtctgaaaaa cgggcagccg atcgagatgc cgatttacga tttcgtgacg cacgcgcgca
     2041 aagacgaaac cgaacacatc gacccgaagc cggtcgtcat tctcgaaggc atcctgattt
     2101 ttgccgaatc gcgcgtgctg gatttgctgg acgtccgcgt ttttgtcgat acgccgagcg
     2161 acattcgcct gatgcgccgc ctgcggcgcg acatcgagca gcgcgggcgc tcgttcgagc
     2221 agacgctctg gcagtatgaa cacacgatta gaccgatgca ttacgaattt gtcgagccgt
     2281 caaagcgcca cgccgacgtg attattcccg aaggcagcca gaccgaaatc agcgtcgaat
     2341 tattgtgcgg tctggtgcgc gaaaaattaa gacgagaaga aaatttagcc gcgaaataga
     2401 cgcgaaaaaa cacgaaaagg aagagcatca atcttttatg ttttttcgcg cgcggtttcg
     2461 cgtctgtttt taaaattctt cgcgtccgtg tggtaagttt attttatgaa tgaagattta
     2521 cggaaattag tcgaagcggc gcgcgacgtg cgcgagcgag cttacgcgcc ttactcggaa
     2581 tttcgcgtcg gcgcggcgat cagaaccaag ggcgggaaaa tttacacggg ctgtaatgtc
     2641 gaatcggcga gctacggtct gacggtttgc gccgaaagag tggcgatttg gaaagccgtc
     2701 tccgaaggcg aaaaggaatt taccgatatc gcagtcgtcg ccgacacgca ggaactgacg
     2761 cctccgtgcg gcgtctgtcg tcaaatcatc tgggaatttt gcaatgatat tccggtgact
     2821 ttcgccaatc tgcacggcaa aaccgaaacc gtacagatga aggaactctt gccgagagca
     2881 tttgatacaa agtttttgaa atgattttca acacaaagac atttgaaatg attttcaaca
     2941 aaaagacaca gaggaaacac aaagaagaat ccgctacgaa cagacgcgaa gaacacgccc
     3001 gggaaattat ttatcctgtt tgaattttct cgtgttcccg gtgtccctgc gccgaacgtt
     3061 ggctttgcgt ttttgtctga aaatgttgta aattcagcta tggaaaagtc gaaaattcta
     3121 gcgctcgttt ttcttttgat tcttataatc cttctgccgt cagtctttgc ccaacgacaa
     3181 tcattaccac gaaaaatcgt tcctaaccgg aatgccgatt tattaatcgt cggcggaacg
     3241 gtcgtcacga tggataaaga ccgtcgcgtg attgaagacg gcgccgtggt ggtcaaaaac
     3301 ggcgagattg cgctggtcgg caaacgcgcc gccgtgacga aaaacctgcg cgcgcgccag
     3361 gttataaacg ccaacggcaa agtcattatt ccaggcttaa tcaacacgca cacgcacatt
     3421 ccgatggtgc tttttcgcgg catttccgac gatttggatt tgaacgaatg gctgacgaaa
     3481 tacatttttc cagccgaagc gaaaaacgtc acggaagatt tcgtgcgcgc cgggacgcgg
     3541 ctcgggctgg cggagatgat tcgcggcggc acgacgacct actgcgatat gtattatttc
     3601 gaagacgcca tcgccgacga aaccgccaaa gcgggcgtgc gcggcgtttt gggcgaaacg
     3661 attatccagt ttccggtcgc cgacaacaaa acgcccgaag acgcgatgcg ctacagcgaa
     3721 agatacgtca acaaatggaa aaatcacgat ttaatcgttc cggcgctggc tccgcacgcg
     3781 ccttacactt tgacgaccga ccatctgcgg gcgataaaaa atctttccga taagctcggc
     3841 gcgcccgtcg tcatccacgt cgccgaaacg cgcagggaag ttgaagacat ttccaaacaa
     3901 tacggcgcgc gtccggtcga atatctgaac cgcatcgggt ttttaaacga tcgaaccatc
     3961 gccgcgcacg tcgttttcgc caacgataac gagattcaga ttttaaagtc gcgcggcgtc
     4021 ggcgtggcgc ataatccgca gtcgaatatg aagctcgcgt ccggcgtttc gcccgttccg
     4081 cagatgttga atgccgatat aagactcggt ctgggcacgg acggcgcggc ttcgaacaac
     4141 gacctgaatt tgtgggaaga gatggacacg gcggcaaagc tgcacaagct ttatgcgaac
     4201 gacccgaaag tcctcaacgc cgagcaagcc tttatgatgg cgaccgtcgg cggcgcgcgg
     4261 gcgctgcatc tggaaaattt aatcggctcg attgaagccg gaaaacgcgc cgacatcgtg
     4321 ctcgtcgatt ttgacggatt gcaccagacg cctttctata acgtctattc tcacctcgtt
     4381 tacgcgacca aagcggctga cgtcgatacc gtcattatca acggaaaaat cataatgcgg
     4441 gaaaaacgtt tgctaacgct cgacgaaaat gctataaaga aagacgcaaa cctttaccgc
     4501 caaaaaatca tcaagagttt atcgaattaa aaagagcagg aatcaaatcg cttgcttgag
     4561 atgcttttaa aaaggcaaaa taaaaagatt gtttgttgaa aaaaagtgaa cgccagttca
     4621 ttttatagaa tgaagccaca gaaggaggaa accgaaatgc cgctaaacgt ccgcctatta
     4681 ggtgctgtcg ttttcttggt cttatttagt gttcttggag ttttagcgca gtcaaccact
     4741 tctgccgatg taatgagagg acgcgtaacg aaggcgaaag cctctatcgc cgtcaaaaat
     4801 tacaacgccg ccatatatga gctcgaagga atccgccgcg aaatcaacga cccgacggtt
     4861 cacagcgtcg tcaacgtgat gttgatgaac tgctatctcg aacagggcga ttacaaacgc
     4921 gctcagggat ttttaaacga gctgtttaac gcgcagaaag ccaataagcc gaacgccgcg
     4981 caaaattatt tcgccgttgc cgcgcaggtc gtcaaaggcg cgcgcaacca gcaggaacgt
     5041 tacaaatcgc tcggcttgat gatgaacgac cgcaatctga cgcccgacgc gattgccgat
     5101 atcgagaaaa tgcgcgaaac cgtcgaactg gttatcacgc agacgaaagc cgccggcacg
     5161 gacaagaaaa aagcttccga cgcttttgct ttgcttgaag aatcgtcgaa cgcgcgaatc
     5221 gctctggcgc gcgacgatta cgacgcgaaa cgctggaaag aagcggtcgc cgacgcgcgc
     5281 gaaggcttga tgagctcgcg cagcacggtt ttgaacgctg ccggcgacga cgaagtgaac
     5341 aacggcacgg tcgcctcaaa tcagccgact tacacgaatt cgacgccgaa cacttctccg
     5401 aaaaccgaaa cgcagccgag tctgattcag acttccaatc aaatcgcgcc gaaaaccgag
     5461 acaaaaacgg aaaacacgac cgaagcgtcg aataatgtta agctcgagcc ggttccggaa
     5521 cggaaacaaa ccgtaacgac tcccgaaaag accgttgcgg aaaccccgaa tacgactgac
     5581 gctccgaaag aaacggttgc cgaaaaacaa ctgacgccga acgtcaatcc cgacaacgag
     5641 ccgaaagttc cgaccagaga acgccgcgtc aatactccga acataagcac ggaaacagtc
     5701 gcgcaaaata cgaatcagac gccgaaagcg ggcgacgata cgcctttgca ggtcggctct
     5761 ctggtcgaat acgcgacgca aaaggtcaat ccggtttacc cgccgcaagc gaaaactctg
     5821 cgaatgacgg gtacggttaa agtcgaagtg atggttgacg agcagggaca agtcagcgcc
     5881 gtgcagaatt cctcgggtcc ttcgctgctg cagcgcgcgg cgacggacgc gctgaaaaaa
     5941 tggaagttca agccgtttac caaagacggt cagccggtca aggcaaaagg ttttgtgaat
     6001 ttcaatttca acctgtaatc tttcgctaaa ttcaaattat taacgggcgg caaccttttt
     6061 cggaaagttg tcgcctaaat tttttgaggc tttctgacta ctgtttcagg tgaaaatata
     6121 aattttatgc ttttaagact tttgtttttc gtgtttctat taacgatgac tgtctatccg
     6181 caaattccgc cggttgaaac gggcgtttcg caaacgctgg cgcgctggcg cgcggctaat
     6241 tattcggacg tgcgttaccg attaaacatc aagctcgaaa agcaagcgcc gctgatgaag
     6301 ggcgagcttg aaattcgcgt gaatctgacc gaagaaggcg cgaaaaacga tttgatttta
     6361 gactggcgca cgacgcagtt tcagaatgat aaagagaagc cgtatgcgaa agtcgtcggg
     6421 gttaataaca aaaccgtctc gctgattggc ggcgtcgata tgcccaatta ttatgtcttc
     6481 aacgagcatc tgggcgtttc gagatttttg ctgaaaacgg gcgagaacgt catcaaaatc
     6541 gagttcgcct cgccgataaa aacgtcgggc gcggctgtca cgcgctatgt cgataaagaa
     6601 gacggcgcgg aatacgtcta ttccttgttc gtgccgtcgg aggcgagcac ggcgtttccg
     6661 gtgttcgacc agccggattt gaaggctcga ttcagtttgt cgctgacggt tccgcgcggc
     6721 tggcgagcaa tttcaaatac cgaagtcgag agtaaattta ctatagacac aacgcattgt
     6781 cgtcaaaact gcgattttga cggcttcgat ttccacgaaa caaagccggc aagcacatac
     6841 gttttcgcct ttgccgccgg tgattttgaa gtctatagag gctgtcttta cggagacaat
     6901 aaaccggaag gcaaaaacgt tccgaactgt ctggaaactt cttcccgcga atcggaaacg
     6961 cgcgactggt ctaaaattta cgttcgcaaa tcgcaagccg aaaaattcaa gccgcacgcc
     7021 gccgaaactt tccgcctcaa ccgcgaagcc gtcaaatatc tggaaacgta tttcgatttc
     7081 aaattcccgt ttccgaaata cgacctcgtt ttgattcccg aatttccgtt taacgggatg
     7141 gagcacgcgg gcgcgacttt tttgcgtgaa tcttccgtaa tttttccgtc cgagccgacg
     7201 aaaaacgatt tactgtcgcg cgccaacgtc atttttcacg aagccgcgca tcaatggttc
     7261 ggcgacacgg tgacgatgcg ttggtttgac gatttgtggc tgaaagaagg cttcgccgaa
     7321 tttatggcgt ataaaacgct ggaaaaggtt ctgcccgaat acaacgcctg gaaagctttc
     7381 tacgagcgca acaaaccgct cgcttacctg accgattcaa ccaaaggaac gacgccgatt
     7441 tatcaggaaa tcccgaattt aaattcggcg aaatcggctt acggcaacat cgtctatcgc
     7501 aaagcgcctt cgtttttaaa acaagcggaa ttttatctgg gcgaaaggga atttcagacg
     7561 gcggtgcgcg cgtttttgcg gaaacatcaa ttttcaaacg ccggttggac tgatttgctg
     7621 tctgaattta acgccgtcac caaaagcgcc tacgtgcggg acttttcgac agcgtgggtc
     7681 aaacagcccg gattattcaa aatcaggttg aatcaaggcg atttcgtaaa ttcggctcaa
     7741 tataaattcg ttttgaacgc cgaagcgcag cactcgattg aacaaaacgc cgagcgcgtt
     7801 atgaaattta aaattctggt catcaatgcg gacggcagca gagacatccg tgagtttatg
     7861 ctgaatccgc agagaagttc tcatagagat acttttcctt tatcgcaaaa tccggcgctc
     7921 gttttcccga actacgagga ttacggctac ggcatttttc tgctcgacga gcgcagccgc
     7981 gcttacgttc tcgaaaatat ccggaacgaa aaagacgatt ttctgcgctc gatgatgtgg
     8041 ggcacgctct gggactcggt gcgcgaaggc gagctgaatc cgagagatta cgtcgaatta
     8101 gccgttaaaa acatcgcggt cgagacggac gagctgacca ttcaaacgat tttaagccgc
     8161 gtttcgaccg cgatgaatta ttatctttcc gacgcgcagc gcgcggagtc ggcgacgaag
     8221 ctcgaagatt tactcgtcga aaaattcacc gccgccaaaa caaaaggaca gcggctgaca
     8281 ttttaccgcg cgtttttgaa catcgcttcg agcaaaaagg cgcagacggt tttgaaggat
     8341 attctgaaag cgaataccgg caaacaagag ccggaagcgc caaagaaaaa ctccgctaaa
     8401 gacgaaaaga gagcggaatt ttttgaattg ccggcgctca gcactaaaga caaattcgac
     8461 atcgtcacgc gccttgccgt cctgggcgac gccgatgcat cgagactgct cgacgaatta
     8521 cagaaaaaag aaacgtccga cgacgcgaaa cgctatgctt acgccgtcgg cgcgggcttt
     8581 ggaacaaaag aagcgaagga aaaatatttt aacgattttc tgaacaataa agaaatttcc
     8641 gaaagctgga ttgaatccgc tttcgtgccg tttaattcgg tgcgtcactc agagctgact
     8701 ttgccgcatc tcgaaaaagc tttggcggaa ttgccgaatc tgaaacgcag ccgcaaaatc
     8761 tttttcgtta acggctggct ggcggcgttt atcggcgggc agaaagacga aaaggcgctg
     8821 tcggcagtca ataagtttct ggcgaataac cccaatctgg acaaggattt gcgcctcaag
     8881 attatggaaa atgtcgacgc gctggaaaga gccgtgaaga ttcgcgccaa atatggaaag
     8941 tgaacggcag gcggcaagcg ataaattgtg aattttgtgt tccgaatctt gagcgcagag
     9001 tgataaacgg cagatgataa atgattttat ttacggttta ccgtttaccg cttattttgc
     9061 ttggcaaatt aaagtttttg gcgcatactt ccctgaaaac gattacaagc cgctcgccgc
     9121 cgtttcgggt tttcaattga atcggctcga aacgtttatc cgataaatat caggtatgta
     9181 attaatatta cagacgcggc gcgcgaatcg gggtaaattc gttctttgaa tatttgcttt
     9241 cggtccggca atttaaaaca acaaaaggag ataggattat ggcaaaaaca atagctacca
     9301 taatgggcat agtttttatt ctgatcggtc tggtcggttt tatttccgat aattttatgg
     9361 gcacgcatct gagcatgacg cacaacctga ttcacctgat ttcaggcgcg gtcgcgcttt
     9421 acctgggctt aaaaggctcg ctttccgccg ccagattatt ctgcctcgcc ttcggcgcgg
     9481 tttatttgct gctcggcttg gcgggatttt tgttcaattc cggcacgacg acgggattgc
     9541 cggcgaatct gaccgacggc ggcaccaata cccatatgct ccacgttttg cccggcgctc
     9601 tcgaactggg cataatggat catatcgtcc atgttctgct cggtctgatt ttcctggtcg
     9661 gaggcttttt gacgcgcgcc gatgtcagaa cggataacgc ccgcgtttaa agtgaaaata
     9721 taatagaaaa gtgaaaagga cggaaaagtg aaaaagcagt ttgaataaac tcgagccgct
     9781 ttttcacttt ttcgcttttc tcaggtgtat gcttcggcgg tttctttctg tttgacggtc
     9841 aaatttccga cggcttccat cggagattta atataatttt tatacttttc aaccagcggc
     9901 gtctccagcc agcgttttcc cgcagcttcg cgatttaatt tataaagacc gagctcgaga
     9961 taaaaatttt ccagcggctc ctgcgaaaaa cggtaacgcc agtcaatcat cgtaaaaagc
    10021 ggaaaccacg tgtagccgat gaccggaacg ccgtcggcgc gcagattttt tatcatcccg
    10081 accgacgatt ccagccagcg ctcgcgcact tcgtccgccc cgaccgcgct ggtttcggta
    10141 atcattatcg gcacttgata acgctcgtga aaatcgcgaa tcaaatcgac aaacccgttg
    10201 cctttcggct cgatgtcgcg aaaagccagc cgcccgcgct tgtccagata aatctgacgg
    10261 gtcgaccatt gcgggtaaaa attcattccg acgacgtcca gagggatttt atttccctga
    10321 agctggtcta aatcgtcgag cgacgcgccg tttctgacga gccacgaaaa aagcaggtgg
    10381 tcgtgcttga tacgcccgga aatcaaatcg tagcaaagat aaccgcgatg cgtttcttca
    10441 ttggcgagca cggctaaatc ttcgcgcacg gtgcgcgtca taccggtcgc ttcgacgtga
    10501 accattaccg tttccgggtc gatttccttg atggcattga ccgtgcggat aatgcctttc
    10561 gccagttgca gcatcacgcg aatataaccc ttttcgcctt tcagataagg cgcccagagc
    10621 ccgcgcatcc cgcacatcag cgcgttgata atcggctcgt ttaaaggcgt gtaccattta
    10681 attaagcctc tgtaccgacg ggcaaattcg gcagcgtagc gcgcgacata ttccggataa
    10741 tcattgtttg caaattcttt tttcagccag aacggcgtgc cgtagtgcat caaatccagt
    10801 atgggcgtga ttttcaaatc ttcgaccaga taggggataa ccttatccgt ccagctccag
    10861 tcgaatttgc cggattccgg ctcgacctta taccacggca cgccccagcg cagcgcgtgc
    10921 aagcccagct cgcgcgccag cacgaggtct tcgcgccaat
//
LOCUS       DASWNE010000327         1795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_258233, whole genome shotgun sequence.
ACCESSION   DASWNE010000327 DASWNE010000000
VERSION     DASWNE010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258233"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1607)
                     /locus_tag="VGB68_06705"
     CDS             complement(<1..1607)
                     /locus_tag="VGB68_06705"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0048955.1"
                     /translation="MAKPAMITVDDDAEVLRAVERDLRRKYAADYRILRADSGQNALD
                     IVRELKIRNNPLALFLVDQRMPGMSGVEFLEQAVKLFPEAKRVLLTAYADTEAAIAAI
                     NEAGINHYLLKPWDPPEENLYPVLDDLLDDWRADYRPPFEGIRVLGNRWSPDAHNIKD
                     LLARNHIPYRWLDVETAETDLEVKQLIDSLDGREKSNLPLLIFPDGTRMAKPRPVEVA
                     EKVGLKTRATEEFYDLAIVGGGPAGLAAAVYGASEGLRTIMIEREAPGGQAGMSSKIE
                     NYLGFPAGLSGGDLTRRAVAQAKRFGVEILAAEAGKICVEGTYRCLELTNGSRVSCHA
                     LLIATGVQWRTLDAPGIERLTGAGIYYGAAMTEAMACRDEDVYIVGGANSAGQSAMYF
                     SRYARQVVMLVRAASLKASMSQYLIDQIKQTPNIRVEYNSSVVAAHGDDHLEAISVHC
                     TASGETDTHPTNSLFILIGAMPRTNWLDGVVERDEHGFILSGKDLMRDGKPPRGWNLD
                     REPGLLETNVPGIFAVGDVRKGSVKRVA"
BASE COUNT          362 a          530 c          487 g          416 t
ORIGIN      
        1 gcgacacgct tgacgctgcc ctttcgcacg tcgccgacgg cgaaaatgcc gggtacgttc
       61 gtttcaagaa gtcccggctc gcggtcgaga ttccaaccgc gcggcggttt cccgtcgcgc
      121 attaaatctt tgcccgataa aataaatccg tgctcgtcgc gctcgacgac gccgtccagc
      181 cagttcgtgc gcggcatcgc gccgatcagt ataaaaaggg aattggtcgg gtgcgtgtcg
      241 gtttcgccgc tggcggtgca atgaaccgat attgcttcca ggtggtcgtc gccgtgcgcc
      301 gcgacgacgg aggaattata ttcgacgcga atattcgggg tttgtttgat ttgatcgatt
      361 aaatactgcg acatcgacgc tttgagcgac gccgcgcgga ccagcatcac gacctgccgg
      421 gcgtagcgcg aaaaatacat cgccgactgc ccggcggagt ttgcgccgcc gacgatgtaa
      481 acgtcttcgt cacggcaagc catcgcttcg gtcatcgccg cgccgtaata aattccggct
      541 cccgtcaatc gctcgattcc gggcgcgtcg agcgtgcgcc actgcacgcc agtcgcaatc
      601 agcaaagcgt gacagctgac ccggctcccg ttggtcagct caagacagcg atatgtgcct
      661 tcgacgcaga ttttcccggc ttccgccgcg agaatctcga cgccgaaacg ttttgcctgc
      721 gcgaccgcgc gccgcgttaa atcgccgccg gaaagaccag ccggaaagcc gagataattt
      781 tcgattttgg agctcatccc ggcttgcccg cccggcgctt cgcgctcgat cataatcgtt
      841 cgcaagcctt cgctggctcc gtaaaccgcc gccgccagtc cggcaggtcc gccgccgaca
      901 atcgccaaat cgtaaaattc ttccgtcgcg cgcgttttta acccgacctt ttcggcaact
      961 tcgaccggtc gcggttttgc catccgcgtg ccgtcgggaa agattaaaag cggcagattg
     1021 gatttttccc gcccgtcgag cgaatcaatc aattgtttga cttccaaatc ggtttccgcc
     1081 gtttcgacat cgagccaacg atacggaata tgattgcggg cgagcaaatc cttgatgttg
     1141 tgcgcgtccg gcgaccagcg gtttcccaaa accctgatgc cttcgaacgg cgggcgataa
     1201 tcggcgcgcc agtcgtccag aaggtcgtcc aaaaccgggt aaaggttttc ttcgggcggg
     1261 tcccagggct ttaataaata atgattgatt ccggcttcgt tgatggcggc gatggcggct
     1321 tccgtatcgg cgtaggcggt caataaaacc cgttttgctt cgggaaaaag tttaacggct
     1381 tgctctaaaa actcgacgcc cgacataccg ggcattcttt ggtcaaccag aaagagcgcg
     1441 agcggattat ttctgatttt taattcgcgg acgatatcga gcgcgttttg cccggaatcg
     1501 gcgcgcaaaa ttcggtagtc cgccgcatac ttgcggcgca agtcgcgctc cacggcgcgc
     1561 agaacttcag cgtcgtcgtc gaccgttatc atcgcgggtt ttgccataat ttctttctgt
     1621 ctttacattt taatttctta tttctctccc ttcaacccac tggcaatgtt ttgtaaaaca
     1681 agtcgtccgg gtagcaataa cgattcctcg cccggctcga aggatttaat gatatagaga
     1741 atcccgctgc gcgccggcag ccaggcgctc atatgccatc gcgtatcgtt gagct
//
LOCUS       DASWNE010000328         1539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_45512, whole genome shotgun sequence.
ACCESSION   DASWNE010000328 DASWNE010000000
VERSION     DASWNE010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45512"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..1110
                     /locus_tag="VGB68_06710"
     CDS             79..1110
                     /locus_tag="VGB68_06710"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEY0048956.1"
                     /translation="METIIAGIMIASLVFYALLGGADYGGGVWDLFAFGKRSKRQREL
                     IARAIGPVWEANHVWLILVIVILFTAFPPAFAAIATALHIPLTLLLIGIILRGTAFTF
                     RTYDVQRDDVQRRWSLVFSIASIITPVLLGMTLGAIASGRIRVENGVVTTGYFNSWLA
                     PFPFAVGFFALALFAFLAAVYLTLETQESDLQEDFRLRALASAAAVGLLALVVFFLAE
                     TGAPTVRAGISRSSWALALHLLTAVSAIGAFYALWTRKYKLARIFAAAQMTLILLGWA
                     LAQFPYLIEPDVTIIAAAAPRITLQLLLGALAAGALLLFPSYYYLFRIFKSETAFTPL
                     SDKTGKINK"
     gene            1116..>1539
                     /locus_tag="VGB68_06715"
     CDS             1116..>1539
                     /locus_tag="VGB68_06715"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0048957.1"
                     /translation="MLKIDIHTHILPKDIPEWNKRFGYGGFIRLEHHKPCCARMLKDD
                     GKFFREVEDNCWSAEKRIEECDRLGVNVQVLSTVPVMFSYWTEATHGAEIAGFLNDGI
                     IEIVRKYPKRFVGLGSVPLQDTKLAVRELERCRKIGLVG"
BASE COUNT          300 a          398 c          428 g          413 t
ORIGIN      
        1 gcttaagctt agacttctaa catttaaacc gtccgcctta acatttaaac cgtccgccgc
       61 ggcagacggt tccgacttat ggaaacgatt attgccggta ttatgattgc ctcgctcgtc
      121 ttttacgcgt tgctcggcgg cgccgattac ggcggcggcg tgtgggactt gtttgccttc
      181 ggtaaacgct cgaaacggca gcgcgagctt atcgcccgag ccatcggtcc tgtctgggaa
      241 gcaaatcacg tctggctgat actcgttatc gttattcttt tcacagcctt tccgcccgct
      301 ttcgcggcaa tcgcgacggc gctgcatatt ccgctgacgc ttttgctcat cggcattatc
      361 ctgcgcggca cggcgtttac gtttcgcact tacgatgttc agcgcgacga cgttcagcgt
      421 cgatggagtc tggtcttttc gattgccagt attatcacgc cggttctttt gggtatgacg
      481 ctcggcgcaa tcgcttccgg tcgcattcgc gtcgaaaacg gcgtcgtcac gaccggatat
      541 tttaattcgt ggctcgcgcc gtttcccttc gctgtcggct ttttcgctct cgcgttgttt
      601 gcttttctgg cggcggttta tctgaccctg gaaacgcagg agagcgattt acaggaagat
      661 ttcaggctgc gagcgcttgc ttccgccgcg gcggtcgggt tgctggcgtt agtcgtattt
      721 tttttagccg agacgggcgc gccgaccgtc cgcgcgggaa taagtcgaag ctcgtgggcg
      781 ctggctttgc atcttttgac cgccgtctcc gcgattggcg cgttttatgc tttgtggacg
      841 agaaaatata aattggcgcg tatttttgcc gccgcgcaga tgacattgat tttgctcggc
      901 tgggcgctgg cgcagtttcc gtatctgatt gagccggacg tgacgataat tgccgccgcc
      961 gcgccgcgta ttacgctgca gcttttgctg ggcgcgctcg ccgccggcgc gcttctgctt
     1021 ttcccgtctt attattattt atttcgcatt ttcaaaagcg aaacggcttt tacgcctctt
     1081 tccgataaaa ctggtaaaat taataaataa gagaaatgct gaaaatcgac attcacacgc
     1141 acattttgcc gaaagatatt cccgaatgga ataagcgatt cggctacggc ggttttatcc
     1201 ggctggaaca tcacaagcct tgctgcgccc gaatgttgaa ggacgacgga aaatttttcc
     1261 gagaggtcga agacaattgc tggagcgcgg aaaaaagaat cgaggaatgc gaccggctcg
     1321 gcgtcaatgt ccaggttttg tcgaccgttc cggtaatgtt cagctattgg actgaggcga
     1381 cgcacggcgc ggaaatcgcc gggtttttaa acgacggcat tatcgaaatc gtccgtaaat
     1441 atccgaaacg attcgtcggt ctgggaagcg tgccgctgca ggacacgaag cttgccgtca
     1501 gagagttgga aagatgcaga aaaatcggct tggtcggcg
//
LOCUS       DASWNE010000329         6072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_222600, whole genome shotgun sequence.
ACCESSION   DASWNE010000329 DASWNE010000000
VERSION     DASWNE010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6072
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222600"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..1019)
                     /locus_tag="VGB68_06720"
     CDS             complement(27..1019)
                     /locus_tag="VGB68_06720"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEY0048958.1"
                     /translation="MSTERDVSTLRIAPKVWRDGELIDWDDARVHVMTHAIHYGSSVF
                     EGIRCYATKRGPAVFRLTEHMQRLLNSAKIYRMDTKITRDELCRGTVELIRQSELEAC
                     YIRPVMFRGLNEERPSFGVNPFPNPVQCYIASWDWGKYLGEEAVESGVDVCVSSWTRI
                     TSNSLPSMAKAGANYMNSQLIKMEAILGGFAEGIALDDRGYVSEGSGENIFLVNNGTL
                     ITPPLGASILPGITRDSVIQIAREMGIPVLETSIQRAALYLADEVFFTGTAAEITPIR
                     SVDRITVGAGKRGEITKRVQDVFFDVIVGEKPAPNNASWLTYVNETSATASVSD"
     gene            complement(1262..3781)
                     /locus_tag="VGB68_06725"
     CDS             complement(1262..3781)
                     /locus_tag="VGB68_06725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048959.1"
                     /translation="MKFRFFLFFAILFLLGNLTNVSAQVDSVIGQVTSSASESFVGGL
                     SGDGRFAVFESNGNVATENPRNTDGNREVFLFDYAQRRIFQITDTRSLLKDQTMPAIF
                     SNTRVEILNIRPVISNDGRWIAFGSNATTSTPTTPNATNPGNFNANSFNVTTGDTTTN
                     PLTSDGNTEMWLYQIPATPPADLSSGDELPFTDLNAGTFIRVTNTAASRLPVEGSTTA
                     LPIIADDNRDASLNDNGNVAAFISNRDLVPCPTTPSATCGNAFPSFDNPEVYAFVRLS
                     STLSQVTATPRGTILAPIYNVNPVVSGNGMRVAFLGNGDNPIAGQTGGANTDRNDEVF
                     YSNLDANGVPTGARVQVTQTAAANVGEIVNIFSYGPRMSRDGRFIAFESFADLETANG
                     ANQNATAVFVYDANTPVTTPATVPFRRIGPRANADSGAAGDVLRFPTFTDYNANGTPA
                     TVLLASRLNIKPDGTIPSTASEGLNPDAARPTQIYSYQLSLPATTAIFTRLTKLPAGT
                     FIASVQPYPSNTQRRISFSMSQVEPGTGNFDLQTETFYLLTPVATATTPANLSFATGA
                     SAIPVTASPVPSPSPTATPTGTPTPTPSVTPTPVTPPAVQGLSIGMLAIVNLDTGFIE
                     PVTPKTAVGSISRSFPLPIELSGVTMTINGAACGLKMVSRRQIMFTVPLGVFPTTGTE
                     VSRPVVINNNGTVIRGNVTIVFARPDIFRSDLAPAAGGRARLQNVVNRVLTTEPFNVT
                     TVRIRGGVRVPTVLRLYATGIFIPNASAVSYSVRIGSTTITLPNTTLAVQREPGVYTI
                     DFTLPPTLRGAGDQPVVLTVTVGGQTFTSRLDDTAPRTSIL"
     gene            4071..5243
                     /gene="lpxB"
                     /locus_tag="VGB68_06730"
     CDS             4071..5243
                     /gene="lpxB"
                     /locus_tag="VGB68_06730"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEY0048960.1"
                     /translation="MNKQSYKIMIVAGEASGDAHAARLVMRLREQAMETDFEFFGATG
                     HLLREAGVETIVNADNLAIVGLPEIAKVLPMFWRAFRALKKTAAERRPDAVILVDFPD
                     FNLKLARALKKSGLKVIYYISPQLWAWRKHRARSIRKYVDLVLAILPFEKDWYAEQGI
                     NHVEYVGNPLAREVYSKLSREEFCAGYNLDAKKPIVALLAGSRHKELARILPAMLETA
                     SLLAKKNPEIQFVNALAATRNLVEVEAAMKETKKKGVSPPENLITVHGETREVLNASD
                     AAAVTSGTATLETAIIGTPMAIVYKTSSLNYKILRPLINVPHFGLINLIARERLAAEF
                     IQNEFTPETLAAELFRLLEPETNRKMRARLKEVVETLGHGAARNAARAILRELEKN"
     gene            complement(5447..5902)
                     /locus_tag="VGB68_06735"
     CDS             complement(5447..5902)
                     /locus_tag="VGB68_06735"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEY0048961.1"
                     /translation="MENNLDYNSMLKTAIEEARKGLDEGGIPIGAALFDKNGNLLGRG
                     HNRRVQEDDPSVHGETDAFRKAGRQRSYRDKIMVTTLAPCWYCSGLVRQFGIGTVVVG
                     ESVNFEGGIEWLRESGVEVIDLNSAECIGMLADFIAANPAVWNEDIGEE"
BASE COUNT         1445 a         1628 c         1659 g         1340 t
ORIGIN      
        1 ccttctccgc gtctgtttct gcgcctctaa tcggagacgc tcgccgtagc cgacgtttcg
       61 ttaacatagg tcagccacga agcgttattc ggcgcgggtt tttcgccgac gatgacgtcg
      121 aagaaaacat cctgaacgcg tttggtgatt tcgccgcgct tgcccgcgcc gaccgtgatg
      181 cggtcaaccg agcgaatcgg cgtgatttcg gcggctgttc cggtaaagaa aacttcgtcc
      241 gccagataca acgcggcgcg ctgaatcgag gtttcgagca ccggaatgcc catttcgcgc
      301 gcaatctgaa tcaccgaatc gcgcgtgatg ccgggcaaaa tcgaagcgcc gagcggcggc
      361 gtgatgagcg ttccgttatt aaccagaaaa atattttcgc ctgagccttc cgacacatag
      421 ccgcggtcgt cgagcgcgat tccttcggcg aatccgccga ggatggcttc catcttgata
      481 agctgcgagt tcatataatt ggcgccggct ttcgccatcg acggcaggct gttggaagtg
      541 atgcgcgtcc agctggagac gcagacgtcg acgccgcttt cgacggcttc ctcgcccaga
      601 tatttgcccc aatcccaact ggcgatgtaa cactgaaccg gattcggaaa cgggttgacg
      661 ccgaaagaag gtcgctcttc gttcagcccg cgaaacataa ccgggcgaat gtaacaggct
      721 tcgagctcgc tctgccggat taactcgacc gttccgcggc aaagctcgtc gcgcgtgatt
      781 ttcgtgtcca tccgatagat tttggcggaa tttaaaagcc gctgcatatg ctcggtcagg
      841 cgaaacacgg ctggaccgcg cttggtcgcg taacagcgaa ttccttcaaa aacgctcgaa
      901 ccgtagtgaa tggcgtgcgt catcacgtga acgcgagcat cgtcccaatc gataagctcg
      961 ccgtcgcgcc aaactttcgg cgcaattcgc aaagtgctga catctctttc agttgacata
     1021 aaacaagatt gctcctttaa ggatttatta taggaagtta ttaaaaaggt agaaactaca
     1081 aaacattgac agatttcaat aaaacgcgaa agaaataaat tttttctttt atttagaata
     1141 aaccaagccg aaggctaaag caagaaagcc gtcggcaggg aaaagtaaaa atgataaagg
     1201 caaaagcagg gaaatcggtt ttctcgcttt tgcctttatc atttcatttt tatgcctctc
     1261 gtcacagaat actggtgcgc ggcgcggtat cgtccagacg actggtaaac gtctgcccgc
     1321 cgaccgtcac ggtcaggact acgggctggt cgcccgcgcc cctcaaggta ggcggcagcg
     1381 taaagtcaat cgtgtaaacg cccggctcgc gctggactgc cagcgtcgtg ttcggaagcg
     1441 tgatggtcgt gctgccgata cgcacggaat aagatacggc agaggcattc ggaataaaaa
     1501 ttcccgtcgc atacaggcgc agaaccgtcg ggacgcgcac gccgccgcga attctgacgg
     1561 tcgtcacgtt aaacggctcg gtcgtcagaa cgcggttgac gacgttttgc agtcgcgccc
     1621 gcccgcccgc agcgggagcc aaatcgcttc tgaaaatgtc cgggcgcgca aaaacgatgg
     1681 tcacgtttcc tctgataacc gttccgttgt tattaatcac cacgggacgc gaaacttcgg
     1741 ttccggtagt cggaaaaacg ccgagcggaa cggtaaacat tatctggcgg cggctgacca
     1801 ttttcaaacc gcaagccgcg ccgtttatcg tcatcgtcac gccgctcagc tcaatcggca
     1861 gcggaaagct gcgggaaatc gaaccgaccg cggttttcgg cgtcaccggc tcgataaatc
     1921 ccgtatccag attaacgatt gccagcatcc cgatagagag tccctgcacg gcgggcggcg
     1981 tcacgggcgt cggcgtcacg ctcggcgtcg gcgtcggcgt gccggtcggc gtcgccgtcg
     2041 ggctcggcga cggaacaggc gatgccgtca ccggaatcgc gctcgcgccg gtcgcaaaac
     2101 tcaggttagc cggagtcgtc gccgtcgcca caggcgtcag caaataaaaa gtttcggttt
     2161 gcaaatcgaa atttcccgtg cccggctcga cctgcgacat actgaaagaa attcttctct
     2221 gcgtattgct cggatacggc tggacgctgg cgataaaagt gcccgccggg agtttcgtca
     2281 aacgagtaaa tatcgccgtc gtcgccggaa ggctcaattg ataggaataa atctgcgtcg
     2341 ggcgcgcggc gtccgggttt aagccttccg aagccgtcga cggaattgta ccgtcgggct
     2401 tgatgttcag gcgcgaagcc aaaagaaccg tcgccggcgt tccgttggcg ttgtaatcgg
     2461 taaaagtcgg gaaacgcaga acgtcgcccg ccgcgcccga atcggcgttg gcgcgcggtc
     2521 cgatgcggcg aaacggaacg gtcgccggag tcgtcaccgg cgtgttggcg tcataaacga
     2581 aaaccgccgt cgcgttttga ttcgcgccgt tcgctgtctc caaatccgca aaagattcaa
     2641 aagcgatgaa tcgcccgtcg cgactcatcc gcggaccgta gctgaaaata ttaacaatct
     2701 cgccgacatt cgccgcggcg gtctgcgtca cctgcacgcg cgcgccggtc ggaacgccgt
     2761 tagcgtccag attcgagtaa aaaacctcgt cgttgcggtc ggtattcgcg ccgcccgtct
     2821 gaccggcaat cgggttatcg ccgttgccta aaaacgcgac gcgcattccg ttgccggaaa
     2881 caaccgggtt gacgttgtag atgggagcca gaatcgtgcc gcgcggcgtc gcagtgactt
     2941 gcgacagcgt gctcgaaaga cgaacgaagg cgtaaacttc cgggttgtcg aagctcggaa
     3001 aagcgttgcc gcaggtcgcc gaaggcgtcg tcgggcaggg aaccaaatcg cggtttgaaa
     3061 taaatgccgc gacatttccg ttgtcgttaa gactggcgtc gcggttgtcg tcggcgataa
     3121 tgggcagcgc ggtggtcgag ccttcgaccg gcaggcggct cgccgccgtg ttcgtcacgc
     3181 ggataaaagt tccggcgttc aaatcggtaa acggaagctc gtcgccgctc gacaaatcgg
     3241 cgggcggcgt ggcgggaatc tggtaaagcc acatttccgt gttgccgtcc gaggtcagcg
     3301 gattggtcgt cgtatctccc gtcgtcacgt taaaggagtt ggcgttgaaa tttcccggat
     3361 tggttgcatt tggagtggtc ggagtcgaag tcgtcgcgtt tgagccgaaa gcaatccagc
     3421 gcccgtcgtt gctgataacc ggacgaatgt ttaaaatttc gactctggtg ttgctgaaaa
     3481 tcgcaggcat cgtctggtct tttaaaagac ttctcgtatc ggtaatttga aaaatacggc
     3541 gttgcgcgta atcgaaaaga aaaacttcgc gattgccgtc cgtgttgcgc gggttttcgg
     3601 tggcgacgtt gccgtttgat tcaaaaaccg caaaacgccc gtcgcccgat aagccgccga
     3661 caaaggattc ggacgccgaa gacgtgacct gaccgataac cgaatcgacc tgcgcggaaa
     3721 cattcgttaa atttccgagt aaaaataaaa ttgcgaaaaa taaaaaaaag cgaaacttca
     3781 cttaagagtc ctccaaaatt ttagaaattc gccggaagcc tctctccagc tgatttcctt
     3841 tgtggggtaa acacaaaaac taaattttaa gcgtattaat gatgcgttgc aaccgcttac
     3901 cgtaaacaaa cgcgaacttt gtcggaaaaa caactggtaa gatgccgcaa ttgacacaat
     3961 tggttgactt gcggaaaact tttatatgag cggcacgtgg taaactttga gcggtaagtt
     4021 ctttattttt cgattgccgc gcggcgtccg tagtttaccg ctttttaata gtgaacaaac
     4081 aaagctacaa aataatgatt gtcgcgggcg aagcttccgg cgacgcacac gcggctcggc
     4141 tcgtgatgcg tctgcgcgag caggcgatgg aaacggattt tgaatttttc ggcgcgaccg
     4201 gacacctttt gcgcgaagcg ggcgtcgaaa caatcgtcaa cgccgataat ctcgcgattg
     4261 tcggtctgcc ggaaatcgcc aaagttctgc cgatgttctg gcgcgcgttt cgggctttga
     4321 aaaaaacggc ggcggaaagg cgacccgacg cggttattct ggtggatttt cccgatttta
     4381 atttaaagct cgcccgcgcg ctgaaaaaat ccggtttgaa agtgatttac tacatctcgc
     4441 cgcagctttg ggcgtggcgc aagcatcgcg cgcggtcgat tagaaaatac gtcgatttgg
     4501 ttctcgcgat tctgccgttt gaaaaagact ggtatgcgga gcagggaatt aatcacgtcg
     4561 aatacgtcgg aaatcctttg gcgcgcgaag tttactcgaa gctttccagg gaagaatttt
     4621 gtgccggata caatctcgac gcgaaaaaac cgattgtcgc gctgctcgcc ggaagtcgtc
     4681 acaaggaact ggcgcgaatt ttgcccgcga tgctcgaaac cgcgagcctg ctggcgaaaa
     4741 aaaatcccga aattcagttt gtcaacgcgc tggcggcgac ccgaaatctc gtcgaagtcg
     4801 aagccgcgat gaaggaaacg aaaaaaaaag gcgttagccc gccggaaaat ctcataaccg
     4861 ttcacggcga aacgcgcgaa gtcttaaacg cctcggacgc ggcggcggtg acgagcggca
     4921 cggcgactct ggaaacggcg ataatcggca cgccgatggc gattgtctac aaaacttcga
     4981 gcctcaatta caaaattcta cgccctttga taaacgttcc gcatttcggc ttgattaatc
     5041 tgattgcgcg cgaaaggctg gcggcggaat ttatccagaa tgagtttacg ccggaaactc
     5101 tggcggcgga gctttttcgt ctgctcgaac cggaaacgaa tcgaaagatg cgggcgcgcc
     5161 tcaaagaagt cgtcgaaacg ctcggacacg gcgcggcgcg caacgcggcg cgggcgattt
     5221 tgcgcgagtt ggaaaaaaat tagcgaggga gaaaatggga atgaaaccac agataaacgc
     5281 agattagaac aggatggaca ggataattta aaaaccaaga acgttgaaat ttccttttta
     5341 tcctgcttat taagccgcga aacctactcc atccttttaa attatccgtg tttatccgcg
     5401 gttaaatttc tctttgtatt tctctgtgtt ctctgtggca aaatccctat tcttcgccga
     5461 tatcttcgtt ccagaccgcc ggatttgccg cgataaaatc ggctagcatc ccgatgcatt
     5521 ccgccgagtt caaatcaatc acctcgacgc ccgattcgcg cagccattcg atgccgccct
     5581 cgaaattgac cgattcgccc acgacgaccg tgcctatccc gaactgccgc accagacccg
     5641 aacaatacca gcacggcgcg agcgtcgtca ccataatttt atcgcggtaa gagcgctgcc
     5701 tgcccgcctt gcgaaaagcg tccgtctcgc cgtgcaccga cgggtcgtct tcctgcacgc
     5761 gccggttatg cccgcgcccc agcagattgc cgtttttatc aaacaacgcc gccccgatcg
     5821 gaatgccgcc ctcgtccaag cccttgcgcg cttcttcaat cgccgtcttc agcatcgaat
     5881 tgtaatcgag attattttcc ataccgaaca atgataggat tcaaaattca aaattcaaaa
     5941 tcctgaatct taaattctga atcctgaatc ttgaatcctg aatcctgaat tattttttaa
     6001 tcagcgggct gctgtgaaag gcgattggct cccagcgtcc tttgcgtttt agaaaggtga
     6061 cggtgttgcg ga
//
LOCUS       DASWNE010000330         2167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_81248, whole genome shotgun sequence.
ACCESSION   DASWNE010000330 DASWNE010000000
VERSION     DASWNE010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81248"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(273..1034)
                     /locus_tag="VGB68_06740"
     CDS             complement(273..1034)
                     /locus_tag="VGB68_06740"
                     /inference="COORDINATES: protein motif:HMM:NF024802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEY0048962.1"
                     /translation="MPDATLITFPPSLDSELSRFLLSHYRIPHQEQRHVIIFSSFYSI
                     WHGSVRFPFLYSDSYALNTVRRIVDHFEPLCPAERKLLPPGDDPEAADADWAFFNGRL
                     GSATSVFGYYHLLPHRQIMIQPLSGGAPQFEVSSVRWAYPVFAGLLRLLLRLTASHAE
                     EALAEIRTVMRIVDERIADGRRYLAGERFSLSDMAFAVAAAPVVFPDDYGGGNTLPPL
                     SETPAELQNLVKEMRELPSGQLALRIYREHRTSGA"
     gene            complement(1535..>2167)
                     /gene="msrB"
                     /locus_tag="VGB68_06745"
     CDS             complement(1535..>2167)
                     /gene="msrB"
                     /locus_tag="VGB68_06745"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEY0048963.1"
                     /translation="GVEQATAASATAVNKTVEAGSPSVINSVNLTKQMEPPTIAAKNT
                     AKIPDKMNSGAQNIQTAPAVKTIKVTSAAGDEDYNGQKILKTDEEWRKHLTPQQFYVL
                     RRKGTEEAFTGKYTDNKKQGTYYCAACGLAVFSSGSKFDSGTGWASFYQPVAASNILE
                     QTDKSLEEERTEILCARCDSHLGHVFDDGPEPTGLRYCINSVALRFKKQK"
BASE COUNT          519 a          564 c          557 g          527 t
ORIGIN      
        1 ttcccctcaa atgcctgtag atttaactag tcgtcggttg aagtgacttt agtttattta
       61 ttaataagtg tcaagatttt ttaaagcttg gcattaaatt cttagcgccc gccggttcga
      121 aagaatttcc ggaaacaaac gcgcgctgcg ccggacaaaa acttttgcgg cggaaattcg
      181 ggcgtcgaaa gtgtttgccg gaaattaatc gctaaagcac cgccgattcc ggtcgccgca
      241 gaagaattgc cgcgcggttt ctctcttccc gattatgcgc ccgatgtgcg gtgttcgcgg
      301 tagatgcgca gagctaattg accggaagga agctcacgca tctctttgac cagattttgc
      361 agctccgccg gcgtttccga caatggagga agcgtgtttc cgccgccgta atcgtccgga
      421 aaaacgaccg gagccgccgc gacggcaaat gccatatcgg aaagtgaaaa gcgctcgccc
      481 gccagatagc gccgaccgtc ggcgatacgt tcgtcgacaa tccgcatcac cgtgcggatt
      541 tcagccagcg cctcttccgc gtgcgaagcc gtcagccgca gcaacaggcg cagcagtccc
      601 gcaaagacag gataagccca ccgaaccgag ctgacctcga attgcggagc gccgccgctg
      661 agcggctgaa tcataatttg gcgatgcggc aacaggtgat aataaccgaa aaccgaggta
      721 gccgagccca atctgccgtt gaaaaacgcc cagtcggcat cagccgcttc agggtcgtcg
      781 ccgggcggca gcagtttgcg ttcggcggga cagagcggct cgaagtgatc gacaatcctg
      841 cgtaccgtgt tcagggcgta agagtcgctg tagagaaacg gaaagcgcac cgagccgtgc
      901 caaatcgagt aaaacgagga aaagattata acgtggcgct gttcctgatg cggaatccgg
      961 tagtggctca ataaaaaacg gctgagctcg ctgtcgagac tgggcgggaa agtgatgagc
     1021 gtggcatcgg gcataggcga ccttcatcta ataaaacatt accgggcgat tttgaaactg
     1081 ataaaaaaca aaaaacgact tcttaaatcc acactgcgaa gcggcaatct gcagcttctc
     1141 atatttaccg gcatttcccc ggaaatgctg atgaatttaa ccgttcgcga ttgaagtgac
     1201 tttaatttat ttattaatga gtgtcaacag attttttgaa tcgagcgtaa aaataaaatt
     1261 ttcggaatag cggtcgatga ttgcccgccg tacccgaatc cggaaccggc gcaggagaaa
     1321 ggcaatttcg gctcgcctgg atttccggtc ggcagaataa tcccgtaggc ttcagcggtc
     1381 gctctctcaa taaagcgatt ggagttttat ctgtcggtct aaataaaaaa gcccgtcctc
     1441 gcgggcgagc ctttctaatc ttattattct cggtcttcgg agaaacctgt ccgtttagtc
     1501 gtttaaacgc cgcgacgagc cggaagcgta ttaactattt ttgcttctta aatctcagcg
     1561 cgaccgaatt tatacaataa cgcagccccg tcggttccgg tccatcatcg aaaacgtgac
     1621 cgagatgcga atcgcagcgg gcgcaaagta tctctgtccg ctcttcttcc agacttttat
     1681 cggtttgctc cagaatattc gaggcggcta ccggctggta gaaacttgcc caacccgtcc
     1741 ccgaatcgaa tttcgacccg gaactaaaca ccgccagacc gcacgccgcg cagtagtaag
     1801 tgccctgctt tttgttatcc gtgtatttgc cggtaaaggc ttcttccgtt ccctttcggc
     1861 gcaatacgta gaactgctga ggcgtcagat gtttgcgcca ctcttcgtcg gttttcaaga
     1921 ttttctgccc gttataatct tcgtcgccgg cagcggaagt gacttttatc gttttgactg
     1981 ccggagcagt ttgtatattc tgcgccccgg aattcatttt atcaggaatc tttgccgtat
     2041 ttttcgcggc aattgtcggc ggctccattt gtttagttaa attaaccgaa ttaataaccg
     2101 atggcgaccc ggcttcgacg gttttattta cggctgtcgc ggaagcagcc gtcgcttgct
     2161 cgactcc
//
LOCUS       DASWNE010000331         1503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_187355, whole genome shotgun sequence.
ACCESSION   DASWNE010000331 DASWNE010000000
VERSION     DASWNE010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187355"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..420
                     /locus_tag="VGB68_06750"
     CDS             40..420
                     /locus_tag="VGB68_06750"
                     /inference="COORDINATES: protein motif:HMM:NF017355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEY0048964.1"
                     /translation="MNKTIKKQVRKTKKESLPKVEQYLYTVGWSDEDEAFVARVAEFP
                     SLAAHGETLEETLKEIKDVVEFVLKDLNESNEPVPEPFGKRSFSGKLVLRMPEYMHRR
                     LALEAMQQGISLNQLLNLKLEASR"
     gene            complement(434..1342)
                     /locus_tag="VGB68_06755"
     CDS             complement(434..1342)
                     /locus_tag="VGB68_06755"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0048965.1"
                     /translation="MSELFTLSLLFSAIRLATPLIFAALGGLFSERAGVINIALEGLM
                     LAGAFTAAIATYELGNPYLGLLCGMAAGAALALIYAIATIKFEADQVVSGMAINFLMF
                     GLPALISGAIYDSAGSTQQIAKENLLPEFFNRISIASLMAFALVPLCWYVLYKTPFGL
                     RLRATGENPAAADAAGVNVIRLRYIAVIISGVLAAAGGAYLSIGQSSLFTRNMTAGRG
                     FIALAALILAKWKPVPVLVACLFFGFTEAMTIQIQGVYKLPSGEDIPVQFIQMIPYVL
                     TIIVLAGFIGLSRAPKALGVPYRKEK"
BASE COUNT          442 a          400 c          377 g          284 t
ORIGIN      
        1 ctttgcgtaa attaaaagaa cttgaagaag ataagaaaga tgaataaaac gattaagaaa
       61 caagtcagga aaacgaaaaa agaaagcttg ccgaaagtcg agcaatatct ttatacggtc
      121 ggctggtctg atgaagacga agcgtttgtc gcccgcgtgg ctgagtttcc ctctctagca
      181 gcgcacggcg aaacgctgga agagacttta aaggaaatta aagatgttgt cgaattcgtc
      241 ctgaaagatt taaacgaatc gaacgagccg gttcccgaac ctttcggcaa gcgctctttt
      301 agcggaaaat tggttctgcg aatgcccgaa tatatgcacc gccgactcgc gctcgaagcg
      361 atgcagcaag gcatttcgct taatcaatta ctaaatttga aactggaagc aagccgctga
      421 acttgcttcc tttttatttt tccttccgat acggaacgcc gagcgctttc ggagcgcgcg
      481 acaagcctat aaagcccgcc agcacgataa tcgttaaaac ataaggaatc atctgaataa
      541 actgcacggg aatgtcttcg cccgaaggca gtttatagac gccctgaatc tgaatggtca
      601 tagcttccgt aaagccgaaa aacaaacagg cgacgagcac cggaaccggt ttccatttcg
      661 ccagaatcag cgccgccagc gcgataaatc cgcgtccagc cgtcatattg cgcgtaaaaa
      721 gcgacgattg cccgatggac agatacgcgc cgcccgccgc cgccagaaca ccggaaataa
      781 tcaccgcgat ataacgcagg cgaatgacgt tgacgccagc cgcatccgcc gccgccggat
      841 tttcgcccgt cgcgcgcagc cgcaagccga aaggcgtttt atacaatacg taccagcaga
      901 gcgggacgag cgcgaaagcc atcagcgaag cgatggaaat gcgattgaaa aattcgggca
      961 agagattttc cttggcaatc tgctgcgtcg agccggcgga atcgtaaatc gcgccgctga
     1021 taagcgccgg cagcccgaac atcaaaaaat ttatcgccat tccgctgacc acctggtcgg
     1081 cttcgaattt gatggtcgca atcgcgtaaa tcagagccag cgccgcgccc gccgccattc
     1141 cgcacaataa gccgagataa ggattgccga gctcgtaggt ggcaatcgcc gccgtgaacg
     1201 cgccggcgag catcaagcct tcgagcgcga tattgataac gccggcgcgc tcggaaaaca
     1261 gcccgccgag cgcggcgaaa atcaaaggcg tggcgagccg aatcgccgaa aaaagcaatg
     1321 acagagtaaa aagttcgctc atatttttcc ccgtttccgc gagtaaattt gaaagcaggc
     1381 gacaaacaaa atgatgattg ccagcaagac ctgtcctaaa tctttggaca ctttcggcgt
     1441 aaaagcgtcg acaaaaattt caccgcgctg caaaaccgca aaaaacaaag ccgcgaccag
     1501 aac
//
LOCUS       DASWNE010000332         2245 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_435082, whole genome shotgun sequence.
ACCESSION   DASWNE010000332 DASWNE010000000
VERSION     DASWNE010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435082"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="VGB68_06760"
     CDS             <1..1124
                     /locus_tag="VGB68_06760"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY0048966.1"
                     /translation="TFQVHPMIQQYLNYYQGRGRQTMEVGLYRSGMFVRMARQIFREE
                     GIPENIVWLGQVESAWKPSALSWAAASGLWQFIPGTGSRFGLRRTAYVDERNGFEKAT
                     KASAQYLKFLANRYNGNWELALAAYNSGEGNVDRAIRRAGVADFWVAYPYLPKETRNY
                     VPNILATILIANNPQRYGFGHVRPAPALNYAQIRVPPSTSLGLLAQAADTSIEYLRYL
                     NPELRSNMSPPEPYNVRVPAGKANEVFAVLRRVPSSNRNNATVAKIVAGETIENVARR
                     TGVSVEQLQAANGSVIPQNGKIIIPNGNNVRKTSYQRPTTPTTAPSGGVRVVKAVNGD
                     TVAKLAARHGASAVEVAKFNGLLPDSQLAPGREIRIPAR"
     gene            1255..2166
                     /locus_tag="VGB68_06765"
     CDS             1255..2166
                     /locus_tag="VGB68_06765"
                     /inference="COORDINATES: protein motif:HMM:NF024931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0048967.1"
                     /translation="MLFRTLSAAVYGIDADLVEIEVNLTPVRGEADNVSPIIIVGLPD
                     LAVRESRERIRAAITNSSYFFPFHKTTINLAPADVRKEGASFDLPIALGILGANGDLG
                     GVEELSDTMSVGELSLDGRIRSIRGALSIALLAREKGIKNLLLPEENAREAAVVQEIS
                     VYPIKNLRDAVELILDLQSGKGTRISPVKIEISDLSAADSRYQVDFAEVRGQHQAKRA
                     LEVASAGGHNILMIGSPGSGKTMLAKRLPTILPPLEFEEALEITKVHSVAGLTGKSGL
                     IHERPFRNPHHTISDAGLIGGSRHKSK"
BASE COUNT          544 a          590 c          620 g          491 t
ORIGIN      
        1 ttacctttca ggttcacccg atgattcagc agtatttgaa ttattatcag ggacgcggac
       61 gccagacgat ggaagtcggg ctttatcgct cgggaatgtt cgtgcgtatg gcgcgccaga
      121 tttttcgcga agaaggcatt ccggaaaata tcgtctggct cggacaggtt gaaagcgcgt
      181 ggaaaccctc ggctctgtcg tgggcggcgg cttccggttt gtggcagttt attcccggca
      241 cgggttcgcg tttcgggctt cgccgcacgg cttatgtcga tgaaagaaac ggttttgaaa
      301 aagcgaccaa agcttcggct caatacttaa aattcctggc aaaccgctat aacggaaact
      361 gggaactcgc gctggcggct tataattccg gcgaaggaaa cgtcgaccgc gccatccgcc
      421 gcgccggagt tgccgatttc tgggtggcat atccttatct gccgaaagag acgcgcaatt
      481 acgtgccgaa cattctggcg acgattttga ttgctaataa cccgcagcgc tacggcttcg
      541 gacacgttcg cccggctccg gcgctgaatt acgcgcagat tcgcgttccg ccctcgacca
      601 gcctcggact gctcgcgcag gcggcggaca ccagcatcga atatctgcgt tatctgaatc
      661 cggagcttcg ctcgaatatg tcgcctcccg aaccgtataa cgttcgcgtt ccggcgggca
      721 aggcaaacga agttttcgcc gttttaagac gcgttccgtc gtcgaatcgc aataacgcga
      781 ccgtcgccaa aatagtcgcg ggcgaaacca tcgaaaacgt cgctcgccgc acgggcgttt
      841 cggtcgagca attacaggcg gcaaacggct ccgtgattcc gcaaaacggt aaaatcatta
      901 tcccgaacgg caataatgtg cgcaaaacga gctatcaacg cccgactacg ccgacaaccg
      961 cgccgagcgg cggagtgcgg gtcgtaaaag cggtcaatgg cgatacggtg gcgaaacttg
     1021 ccgcccgtca tggcgcatcg gcagtcgaag tcgctaaatt taacggcttg ctgcccgatt
     1081 cgcagcttgc cccgggacgc gaaatccgca ttcccgcccg ttaagattaa gtcgaagaaa
     1141 atgaaaaaag tcgagcctga tttatcgggc tcgacttttt ttccttgaaa aataattcgt
     1201 ttaatgatag aaaaaacaat attcaattaa cctgaaatgt taagcccata gattatgctg
     1261 tttcgcacgc tatcagccgc cgtttacgga attgacgccg acctcgtgga gatcgaagtc
     1321 aatttaacgc ccgtgcgcgg cgaagccgat aacgtttcgc ccattattat tgtcggtttg
     1381 cccgatttag ccgttcgcga atccagagaa agaatccgcg ccgccatcac aaacagctcc
     1441 tatttcttcc cgtttcataa gacgacgata aatctcgcgc ccgccgacgt gcgcaaagaa
     1501 ggcgcgagct ttgatttgcc gattgcactg ggaatcctgg gcgcaaacgg cgatttgggc
     1561 ggcgtcgaag aattgagcga tacgatgagc gtcggcgagc tttcgctcga cgggcgaatt
     1621 cgctcgattc gcggcgcgct ttcgattgcg cttttggcgc gcgagaaagg catcaaaaat
     1681 ctgcttctgc ccgaagaaaa tgcgcgtgaa gccgcagtcg tgcaggaaat aagcgtttac
     1741 ccgataaaaa atctgcgcga cgccgtcgaa ttgattctgg atttacagag cggaaaggga
     1801 actcgcatct cgccggtgaa aatcgagatt tccgatttga gcgccgcgga cagtcgctat
     1861 caggtcgatt tcgcggaggt gcgcgggcag catcaggcaa aacgcgcgct cgaagtcgcc
     1921 agcgcgggcg gtcataatat tttgatgatc ggcagcccgg gttcgggcaa aacgatgctg
     1981 gcgaagcgtc tgccgacgat tctgccgccg ctcgaatttg aagaagcgct ggaaatcaca
     2041 aaagttcatt cggtcgccgg tctgacggga aaatccggct tgattcacga gcgaccgttt
     2101 cgcaatccgc accacacgat ttccgacgcc ggtttgatcg gcggtagtcg gcacaaaagt
     2161 aaatgagact gagcaaaagt aatgagacta gagcaagagt agtgagactt aaaagatatt
     2221 gatttatcag atagtgtcct aatag
//
LOCUS       DASWNE010000333         2363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_324953, whole genome shotgun sequence.
ACCESSION   DASWNE010000333 DASWNE010000000
VERSION     DASWNE010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324953"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1475
                     /locus_tag="VGB68_06770"
     CDS             <1..1475
                     /locus_tag="VGB68_06770"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0048968.1"
                     /translation="MMGRGGGFGTTFGAQTSIGLLPILYFGSDELKEKWIPRIVSGEV
                     VTAYCLSEAGSGSDALGAKCSARLSEDGSEYTLNGEKMWISNGGFADVYIVFAKVDGA
                     KEKFSAFVVERSENCRPGAEEHKMGIKSSSTTPLILSDARIPVGNLIGNVGDGAKIAF
                     NILNVGRFKLGASVTGGAKLALFEAIRYANERHQFNKPISGFGAIKHKLAEMAIRTWV
                     AESITYRTVGMIDSLTGEDGGDKEKKLRSIEEYAVESSINKVACSEALDYVVDEMVQI
                     YGGYGYSADYPAEKAYRDSRINRIFEGTNEINRMLIPGQLMKRAMKGRLGLLQAAKAL
                     QDEIFNPQMSFDEDDSLLATETKLAGNAKKVALMILGTAAQKYMMELQNQQEILLNAA
                     DIIIDAYQMETAILRAKKLAEKGSADRQVDMAQVFCNDAIQRVEAKAKNTLAAMSEGD
                     ELRTLLVALKRFTKNNSPVNTIAARQRIADTLIQANKYVF"
     gene            complement(1613..>2363)
                     /locus_tag="VGB68_06775"
     CDS             complement(1613..>2363)
                     /locus_tag="VGB68_06775"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0048969.1"
                     /translation="LRSSTQDWTPVHFGAAGDKLAPGDYDGDGKTDLTVYRGGQWFIL
                     ESGSGNFRALQFGLPDDVPTPADFDADGRTDIAVFRPSNGYWYFTLSSDNQFRAVQFG
                     ASGDKPVIGDYDADGRADQAVYRPSNGYWYVLRSTLGFNAVQFGVASDRPVQGDYDGD
                     GKTDFAVFRPADGTWYLLRSQAGFAARQFGISTDEPAPGDYDGDGKFDLGIYRNGMWY
                     LRLSQSGFDTARQFGAPGDKPIPAAYIPAGN"
BASE COUNT          575 a          633 c          675 g          480 t
ORIGIN      
        1 aaatgatggg acgcggaggc ggcttcggca cgacgttcgg cgcgcagact tccatcgggc
       61 ttttgccgat tctttatttc ggctcggatg agctgaagga aaaatggatt ccgcgcatcg
      121 tttccggcga ggtcgtcacg gcttactgcc tgtcggaagc cggttcgggt tctgacgctc
      181 tgggcgcgaa atgcagcgcg agactcagcg aagacggcag cgaatacacc ttgaacggcg
      241 agaaaatgtg gatttccaac ggcggtttcg ccgacgttta catcgttttc gctaaagtcg
      301 acggcgcgaa agaaaagttt tcggctttcg tcgtggaaag aagcgaaaat tgccgtcccg
      361 gcgcggaaga acacaaaatg ggcattaaat cttcttcgac gacgcctctg attttgtcgg
      421 acgcgcgcat tccggtcgga aacttgatcg gaaacgtcgg cgacggcgcg aaaatcgctt
      481 tcaatatttt aaacgtcggt cgtttcaaac tcggtgcatc ggtgaccggc ggcgcgaaac
      541 tcgctttgtt tgaagcgatt cgctacgcca acgaacgtca tcaattcaac aaaccgattt
      601 ccggtttcgg cgccatcaaa cataagctgg cggaaatggc gattcgaacc tgggtcgccg
      661 aatcgatcac gtatcgcacg gtcggaatga tcgactcgct caccggcgaa gacggcggcg
      721 acaaggaaaa gaaactgcgc tcgattgaag aatacgcggt cgaatcttcg attaacaaag
      781 tcgcctgctc ggaagcgctc gattacgtcg tcgacgaaat ggtgcaaatc tacggcggtt
      841 acggctattc ggcggattat ccggcggaaa aagcgtatcg cgattcgcgc atcaatcgca
      901 ttttcgaagg gacgaacgaa atcaaccgga tgctgattcc cggacagttg atgaagcgcg
      961 cgatgaaagg tcggctcggt ttattgcagg cggcaaaagc tttgcaggac gagattttca
     1021 acccgcaaat gtcgtttgac gaagacgaca gccttttggc aacggagacg aagctcgccg
     1081 gaaacgcgaa aaaagtcgct ctgatgattc tcggcacggc ggcgcagaaa tatatgatgg
     1141 agctgcaaaa ccagcaggaa attctgctga atgcggcaga cattatcatc gacgcgtatc
     1201 aaatggaaac cgcgattttg cgcgcgaaaa aactcgccga aaaaggcagc gccgaccgcc
     1261 aggtagatat ggcgcaggtt ttctgcaacg acgcgattca gcgcgtggaa gcgaaagcga
     1321 aaaacacgct cgccgcgatg agcgaaggcg acgagctgcg aactttgctg gtcgcgctga
     1381 aacgctttac gaaaaacaat tcgcccgtca atacgattgc agctcgtcag agaattgccg
     1441 atacgctgat tcaggcaaat aaatacgttt tttagagtga atcaaagacg cgcgcgtgca
     1501 gcgcagtcgc attccaaaca gaaaaacggc gactagggac ggcggcttcc ggttttaatt
     1561 tcgaccggag ccgccgtcta tccgtttgaa aatgtgtttt tgaatctcgc gttcaatttc
     1621 cggctggaat ataagctgcc ggaatcggtt tatcgcccgg agcgccgaat tgccgcgccg
     1681 tatcaaaacc gctttggctc agccgcaaat accacatccc gttgcgataa atgcccaaat
     1741 cgaatttgcc gtcgccgtcg taatcgccgg gcgcgggctc gtcggtcgaa atgccgaact
     1801 gcctcgcggc aaatcccgcc tggctgcgga gcaaatacca cgttccgtcc gccggacgga
     1861 aaaccgcgaa atcggttttc ccgtcgccgt cgtaatcgcc ttgaacggga cggtcagaag
     1921 cgacgccgaa ttgaacggca ttaaatccga gcgtgctgcg cagaacatac cagtagccgt
     1981 ttgacggacg ataaacggct tgatcggctc gcccgtcggc atcataatcg ccgattactg
     2041 gtttgtcgcc gctcgcgccg aactgtacgg cgcggaactg attgtcggag ctgagagtga
     2101 aataccagta accgtttgac gggcgaaaaa cggctatgtc cgttcgcccg tcggcgtcga
     2161 agtctgcggg cgtcggcaca tcgtccggca aaccgaactg cagggcgcgg aagttgccgc
     2221 ttccgctttc caggataaac cactgaccgc cgcggtaaac cgtcaaatcg gttttaccgt
     2281 cgccgtcgta atcgcccggc gcgagcttat cgcccgccgc gccgaaatgc acgggcgtcc
     2341 agtcctgcgt cgaggaacgc agc
//
LOCUS       DASWNE010000334         8103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_11030, whole genome shotgun sequence.
ACCESSION   DASWNE010000334 DASWNE010000000
VERSION     DASWNE010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8103
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11030"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="VGB68_06780"
     CDS             complement(<1..1075)
                     /locus_tag="VGB68_06780"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HEY0048970.1"
                     /translation="MERQFSGFNVRLLAFVLVFSIIFSACGSESASNSNGTSRTETQN
                     ADVYYDKSAPVMAGNTSNTMAVDRQAMYDDEKSAGGERYAEIEENPFLETSRAPLSTF
                     SIDVDTASYANVRRFLNQGQLPPKDAVRIEELINYFEYDYPQPLGDAPFSVNNEVAVC
                     PWNARHKIVQIGLQGKKVSLDNAPPSNLVFLIDVSGSMESPDKLPLLKDSMRVLVNQL
                     KPQDRVAIVVYAGKSGLVLPSTPAADKGEILSALNNLESGGSTNGGQGIQLAYRVAQE
                     NFIGNGNNRVILATDGDFNVGLTGDNELVSLIENKRESGIYLSVLGFGTGNTNDSMME
                     KLADKGNGNYSYIDSQDEARKALG"
     gene            complement(1199..1756)
                     /locus_tag="VGB68_06785"
     CDS             complement(1199..1756)
                     /locus_tag="VGB68_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048971.1"
                     /translation="MRKHILGLALFGLTVGFSIGVYKLLSFMSFSQNLEVAVVQPSVK
                     TFDKNSEPEKAGKNSFTISQVVLYPKESEKNIDVELTANCKKVRCSGVHYSINLYFFT
                     KNESGTEFLGAETLIFEPGSRNNEAYVTNLPVDSEWLQRLKPKDNLYVTLERKTNDIT
                     GDVYERPSSDMSFAKPVLLMWGKKK"
     gene            complement(1825..2370)
                     /gene="cyaB"
                     /locus_tag="VGB68_06790"
     CDS             complement(1825..2370)
                     /gene="cyaB"
                     /locus_tag="VGB68_06790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HEY0048972.1"
                     /translation="MAIEIEKKYRLNAAQCERISSALSEIGAQFQGVDFEENTLYRGG
                     VLDEKNAVLRVRKIGGRTILTYKRRIRNEFDVKQQIEYETEIGSAEEIENIIENLGFV
                     RSLVYEKRRRTWQFRQVEIVLDELPFGLFMEIEGSITAIAEAEMFLEAEDFTVEHETY
                     PNLTGRHGIKNGEVVEARFER"
     gene            complement(2459..3811)
                     /locus_tag="VGB68_06795"
     CDS             complement(2459..3811)
                     /locus_tag="VGB68_06795"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0048973.1"
                     /translation="MRKFLAVVKREYKKVVFTWAFLIGTLLAPFIAAMFGVIPAILFS
                     SKGERQRIAIVDQTGTVGARLKENLSAEKVSEKVQEAAKEAFKDLDESQQERMKRGAQ
                     QLGESFVFVDYDSEGKTTEQIRRELNAQIQEQKIDAYLIIPQSFDAPDAEFEYYSRNP
                     GDFVTTANLENALNEAVRSERLARANISEAQLKDLSRNVAFEKTKVSEKGEEKENDGK
                     FTAAFVIGVLIYITLAIYGQQILGAVVEEKETRIAEILFSSAKPFELMMGKLVGVGLA
                     GLTQLAIWLSSALILLVFGATTMDAAGIPFSMPNITPLSIIYFLTFFMLGFCLYATIF
                     ALIGSMVTTVQEGGQFAMLPVIILLAGFYCIFPIVRDPNSTVSFWVSIAPFLATITMP
                     VRIAIETPPFWQIALSVLFNVAAIFGLVWVAARVYRVGMLMYGKRATIPEVWKWIRQS
                     "
     gene            complement(3804..4721)
                     /locus_tag="VGB68_06800"
     CDS             complement(3804..4721)
                     /locus_tag="VGB68_06800"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0048974.1"
                     /translation="MSEQSVTLKVENVTKRYGEFTAVDSISFNVRAGRVFGFLGPNGA
                     GKTTTIRMIVGITAPDEGSIELFGQKISSALQDRIGYLPEERGLYKKMKIFEQLRYFA
                     ALKNVRRKQADERIDFWLERMNLQAWKKKKANDLSKGMQQKIQFVSSLLHNPDLLILD
                     EPFSGLDPINVETLLEIIAELKAGGKTIIFSTHLMETAERLCDDIILINKSRKIVGGT
                     LREVKESFGKNLIALRAIGGEAVLRDKNLVSNVIEHADERELQLAEKADAQVLLKKLI
                     ESGAAITKFELIEPSLNDIFIEKVSETNA"
     gene            5049..6062
                     /gene="corA"
                     /locus_tag="VGB68_06805"
     CDS             5049..6062
                     /gene="corA"
                     /locus_tag="VGB68_06805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEY0048975.1"
                     /translation="MEIFVYHKGTDRIEEDYSRDQLPRLLQNKENLIWIDMEQPTAEE
                     TQILSDVFHFHPLTIEDAVETRNHPKVEPYSNYLFLIVHGVSMDTNSSNFITKELDIY
                     LGENFVLTYHHQPFLSINAVKGQVRTNTFSCQRGSDYLLHQILDQLVDLYIPVVDDFD
                     AAINDIEDRIFQMKKGNNKILEEIMDLKRSVARLVRISSKQLDVLSRLARGEFPLIDE
                     NVLPFYRDVYDHLHRVSVLAENYRDLVGGLLNIHFNVVANRTNDVVKILTIFSAIMLP
                     LSLIAGIYGMNTKLPDAENPNSFWHIIGIMILFGAVLLLWFWRKGWIFQKDDEVEDKK
                     TEN"
     gene            complement(6094..6822)
                     /locus_tag="VGB68_06810"
     CDS             complement(6094..6822)
                     /locus_tag="VGB68_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048976.1"
                     /translation="MKIHVLAGDALANDFNNTGIEGERVVCRECLVEGDVRAENLEDF
                     WKLRAGFIKSAYGENEESYFGKVVAEFEKLKNLAPAAEVNLWFEYELFCQANMWFCLY
                     LLQNTGASVYRVAPVVRTEEDIWKGFGKLGSADLKKCFAERIKFGEEDIRLGARLWKA
                     YQNADYEKLENFSETESDCFPRLREVCRAEIEKDFRPQKILREIIENGSRDFAEIFPE
                     FSVRAGVYGFGDAQVKRILNQVEA"
     gene            complement(6819..7427)
                     /locus_tag="VGB68_06815"
     CDS             complement(6819..7427)
                     /locus_tag="VGB68_06815"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEY0048977.1"
                     /translation="MPGYDELIHISASVERAELAKEEIGEKSFLDYFSLAVTTFGVGY
                     IPLAPGTWGSAVGVLIYLGFCQIEASGAAYLALRGWQEAQIIAWIHVFNAILFLLFCL
                     LGIWAASRATKLFKHKDPSQAVVDEVIGQLLVFLFVPFDISWKYILAGFLLFRLFDIW
                     KPYPIDSLQSLPAGIGVCADDILAGIYAGAILSLFYAIGLML"
     gene            complement(7599..>8103)
                     /locus_tag="VGB68_06820"
     CDS             complement(7599..>8103)
                     /locus_tag="VGB68_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048978.1"
                     /translation="APVTLKDLAIDFPDVEGWERDEIQKYPSEELGFSVNYMSEEGGR
                     VTIYVYNGGKSNIPNDLNDKILKNEFANARNEIQKVADMGYYQNVKEVKNDTVTLGGA
                     SGKIKALRALYNLSAGGRNLTSEIYIFAHQNRFIKIRATRPRDKDESGNKAFANLLAE
                     IDAMFSK"
BASE COUNT         2026 a         2086 c         1812 g         2179 t
ORIGIN      
        1 cgccgagcgc tttgcgggct tcgtcctgcg agtcgatgta ggaataattg ccgtttccct
       61 tgtcggcgag cttttccatc atcgaatcgt tggtgttgcc cgtgccgaag ccgagcaccg
      121 aaagataaat tccgctctcg cgcttgttct cgattaagct gaccagctcg ttgtcgccgg
      181 tcaagccgac gttgaaatct ccgtccgtcg ccagaatcac gcggttatta ccgttgccga
      241 taaaattttc ctgcgcgacg cgataagcga gctgaattcc ctgcccgccg ttggtcgagc
      301 cgcccgattc caaattgttc aaagccgaaa gaatctcgcc tttatcggcg gcgggcgtcg
      361 aaggcaaaac caagccgctt ttccctgcgt agacgacgat tgcgacgcgg tcttgcggtt
      421 ttagttgatt gaccagaacg cgcatcgagt ctttcaaaag cggaagctta tcgggcgatt
      481 ccatcgagcc ggaaacatcg attagaaaaa ccagatttga aggcggcgca ttgtcgagcg
      541 agactttttt gccctgcaat ccgatttgca cgattttgtg tctcgcgttc cacgggcaga
      601 cggcgacttc gttatttacg gaaaacggcg catcgcccag cggctgcggg taatcgtatt
      661 caaaataatt gattaattct tcgatgcgca ccgcgtcttt cggcggaagc tgtccctgat
      721 tcaaaaagcg tcgcacgttt gcataagaag ccgtgtcgac gtcaatcgaa aatgtcgaaa
      781 gcggcgcgcg cgaagtttcc agaaaaggat tttcttcgat ttcggcgtag cgttcgccgc
      841 cggcgctttt ttcatcgtca tacatcgcct gtctatcgac agccattgta ttcgacgtgt
      901 taccagccat tacgggcgcg cttttgtcat agtaaacatc ggcattttgg gtttcagtcc
      961 gactcgtgcc gttgctattc gacgcgcttt cgctgccgca agccgaaaaa attatcgaaa
     1021 acaccagaac aaacgccaac aatctaacat taaagcctga aaactgtctt tccatatttc
     1081 ctccaaaaaa attctgcaag ttctgaaaac tcgcttttcc ctgattcctg ttacgccgaa
     1141 cagaacggag cgcgatattc aaacttgcag aaaattttga agctaaaatt ttaaaaggtt
     1201 attttttctt tccccacatc agcagaacgg gctttgcaaa agacatatca gaactgggtc
     1261 tctcgtagac gtctccggta atatcgtttg ttttcctttc taaagtgaca tacagattat
     1321 ccttcggttt caatctttgc agccattcag aatcaaccgg cagattagta acatacgctt
     1381 cattatttcg agagccgggc tcaaaaatga gagtctcggc tcctaaaaat tccgttccgc
     1441 tttcgttttt cgtaaaaaaa tacaagttaa ttgagtagtg aacgcccgaa catctgacct
     1501 ttttacagtt tgccgtgagc tcgacatcga tatttttttc agattcctta ggatataaaa
     1561 ccacttgaga aattgtgaat gaatttttgc cggctttttc cggctccgaa tttttatcaa
     1621 aggttttaac cgaaggctga acgacggcga cctccaaatt ttgcgaaaaa ctcataaaag
     1681 acagcaattt ataaaccccg atactaaaac cgacggttaa gccgaaaagc gccaaaccca
     1741 gaatatgttt tctcattgcc aagactctcc tttttccgac agaacgagat taaagcatta
     1801 tcttgcagaa gaactggaaa atttttaccg ttcgaaacgc gcttcgacga cctcgccgtt
     1861 ttttattccg tgtcttcccg ttaaattcgg atatgtttcg tgttcgaccg taaagtcttc
     1921 ggcttcgaga aacatttcgg cttcggcaat cgccgttatc gagccttcga tttccataaa
     1981 aagtccgaaa ggaagctcgt ccagaacgat ttcgacttgc cggaactgcc acgtccgccg
     2041 ccgcttttcg taaaccagag agcgtacgaa accgagattt tctattatgt tttcgatttc
     2101 ttccgcgctg ccgatttcgg tttcgtattc gatttgctgt ttgacgtcga actcgtttcg
     2161 gatgcgccgc ttgtaggtca gaatcgttct gccgccgatt ttgcggacgc gcagaaccgc
     2221 gtttttttcg tcgagaacgc cgccgcgata aagcgtgttt tcttcaaaat cgacgccttg
     2281 aaactgcgcg ccgatttccg acagcgcgga cgaaatccgc tcacactgcg ccgcgttcag
     2341 acgatatttt ttttcgattt caatcgccat ttatttaatt cgggattcag gattcaggat
     2401 tcaggattag gaaacggagt ttaattctga atcctgaatc ctgaatcctg aattaatctc
     2461 agctttgccg aatccatttc cacacctcgg gaatcgtcgc ccgcttgccg tacatcaaca
     2521 ttccgacgcg ataaacgcgc gccgctaccc agaccagccc gaaaattgcc gccacgttaa
     2581 ataaaaccga cagcgcaatc tgccagaacg gcggcgtttc gatggcaatc cgcaccggca
     2641 tcgtaatggt cgccagaaaa ggcgctatcg aaacccagaa cgaaaccgtc gaattcgggt
     2701 cgcggactat cggaaagata cagtaaaaac cggcgagcag gataatcacc ggaagcatcg
     2761 caaattgccc gccttcctgc acggtcgtga ccatcgagcc gattagagcg aaaatcgtcg
     2821 cgtacagaca aaatccgagc ataaagaagg tcagaaaata gataatcgaa agcggcgtga
     2881 tgttcggcat cgaaaacggg attcctgccg catccattgt cgtcgcgccg aaaacgagca
     2941 gaataagagc cgaggaaagc cagattgcca gctgcgtcaa gcccgccagc ccgactccga
     3001 ccagtttgcc catcatcaat tcgaatggtt tcgccgaaga aaaaagaatt tcggcaatgc
     3061 gcgtttcctt ttcttcgacc accgcgccga gaatttgctg cccgtaaatt gccagcgtga
     3121 tgtaaatcaa aaccccgatg acaaaggcgg ctgtaaattt cccgtcattt tctttttcct
     3181 cgcccttttc gctgactttc gttttctcga aagccacgtt tcggctgagg tcttttaatt
     3241 gcgcctcgct gatattggct ctcgccagac gctcggaacg caccgcttcg ttaagagcgt
     3301 tttcaagatt cgcagtggtt acaaaatcgc ccggattgcg cgagtaatat tcaaattccg
     3361 catccggcgc gtcaaagctc tgcgggataa tcagataagc gtcgattttc tgctcctgaa
     3421 tttgcgcgtt cagctcgcgg cggatttgct cagtcgtttt cccttcggag tcgtaatcga
     3481 caaacacgaa gctttcgccg agctgctgcg cgccgcgctt cattctctct tgctgcgatt
     3541 cgtcgaggtc tttgaaagct tcttttgccg cctcctgcac tttctcggaa actttttccg
     3601 ccgaaagatt ttctttcagc cgcgccccga cggttccggt ctggtcgacg atggcgattc
     3661 gctgcctctc gcctttgctt gaaaacaata tcgccggaat gacgccgaac atcgccgcga
     3721 taaagggcgc gagcaatgtc ccgattaaaa aagcccaggt gaaaacgacc tttttgtatt
     3781 cgcgcttgac caccgccaga aatttacgca tttgtttcgc tcactttttc gatgaaaata
     3841 tcgttcagac tcggctcaat cagttcgaat ttcgtaatcg cggcgcccga ttcgattaat
     3901 tttttgagca aaacctgcgc gtccgctttt tccgccagct gcaattccct ttcgtcggcg
     3961 tgttcgatga cgtttgaaac aaggttttta tcccgcaaaa cggcttcgcc gccaatcgcc
     4021 cggagcgcaa tcaggttttt tccaaagctt tcctttactt cgcgcaacgt tccgccgacg
     4081 atttttcgcg atttgtttat caggataatg tcgtcgcaca gacgctcggc ggtttccatc
     4141 aggtgcgtcg agaaaattat cgttttgccg ccggctttca attcggcgat gatttccagc
     4201 agcgtctcga cgtttatcgg gtcgagcccg gaaaagggct cgtccagaat cagcaaatcc
     4261 ggattgtgca gcaaggagga gacgaattgt attttctgct gcattccttt cgacaaatca
     4321 ttcgcctttt tctttttcca cgcctgcaaa ttcatccgtt cgagccagaa atcgatgcgt
     4381 tcgtcggctt gttttcgccg cacgtttttc agtgcggcga aatagcgaag ctgctcgaag
     4441 attttcatct ttttataaag cccgcgttct tccggcaaat aacctatgcg atcctgcaat
     4501 gcggaagaaa tcttttgacc gaaaagctcg atgctgccct cgtccggcgc ggtgatgccg
     4561 acaatcatcc ggatagtcgt cgttttgccc gcgccgttcg gaccgagaaa gccgaaaacc
     4621 cgaccggcgc gaacgttgaa gctgatagaa tcgaccgccg taaattcgcc gtaacgcttg
     4681 gttacgtttt cgactttcag tgtcacgctt tgctcgctca taaaagatgc tgacagatta
     4741 gcacagatta aaaaaagttt tactatcaaa caatttagcc gagctgataa acaagatttt
     4801 atcccggacg ttaaactttc gccctgaata taaaattttc gacctcaata tcaagctttc
     4861 gctctgaatc cggagctttc gttccgagta tcgaatattt ggcgcgccgg cggcaatttt
     4921 cgcttgtttt cggctttgtc cgagactcaa acaatttcga aatcgtgcaa ataatcgcat
     4981 cgctgtttgt cagcgattgg ctgtcgatga taaaagattt gttggcttta aataatttaa
     5041 cttcaattat ggaaattttt gtatatcaca aaggcacaga ccgcatcgaa gaagactatt
     5101 cgcgcgacca attgccccgg ctgctccaga ataaagagaa tttaatctgg attgatatgg
     5161 agcagccgac cgcggaagag acccaaattc tgagcgatgt ttttcatttt catccgctga
     5221 cgatcgaaga cgccgtcgaa accagaaatc acccgaaggt cgaaccttac agcaattatc
     5281 tttttttaat cgttcacggc gtttcgatgg acaccaactc gtcaaatttc ataaccaaag
     5341 agctcgacat atatctggga gaaaattttg ttctgacgta tcatcatcag ccgtttctca
     5401 gtatcaatgc cgtcaaaggt caggttcgca ccaatacgtt ttcgtgccag cgcggctcgg
     5461 attacctgct gcatcaaatc cttgaccagc tggtggattt atatattccg gtcgtcgatg
     5521 atttcgacgc ggcgataaac gacatcgaag accgcatttt tcaaatgaaa aaaggcaata
     5581 ataagatttt ggaagaaata atggatttga aaagaagcgt cgcgcggctt gtcagaatca
     5641 gctccaagca gctcgacgtt ctctcgcgtc tggcgcgcgg cgaatttccc ctgatagatg
     5701 aaaacgttct gcctttttac cgcgatgttt acgaccatct gcaccgcgtt tccgtgctcg
     5761 ccgaaaatta tcgcgatttg gtcggcggtc tgctcaatat tcattttaac gtggtcgcca
     5821 atcgcaccaa cgacgtcgtc aaaatcctga cgattttttc ggcgattatg ctgccgctgt
     5881 cgctgattgc aggaatttac gggatgaata ccaaattgcc cgacgccgaa aacccaaaca
     5941 gtttctggca tatcatcggc ataatgattt tgttcggcgc agtgcttttg ttgtggttct
     6001 ggcgcaaagg ctggattttt caaaaagatg acgaagtgga agacaagaaa acagagaact
     6061 gagaactgat atttggagaa gctgcttcag ttatcaggct tcaacctgat ttaaaatcct
     6121 tttcacctgc gcgtcgccga agccgtagac gccggcgcgg acggaaaatt cgggaaagat
     6181 ttcggcgaaa tcgcgggagc cgttttcgat tatttcccgc aggattttct gcgggcgaaa
     6241 atctttttca atctcggcgc ggcacacttc tctcagacgc ggaaaacaat ccgattccgt
     6301 ttcggaaaaa ttttccagct tttcataatc cgcattttga taagctttcc agagccgcgc
     6361 gccgagccgg atgtcttcct cgccgaattt tattctttcc gcaaaacatt tttttaaatc
     6421 tgccgagccc agctttccga aacctttcca gatgtcttct tccgttctga caaccggcgc
     6481 gacgcggtaa acgctcgccc cggtattctg caaaagataa agacaaaacc acatattcgc
     6541 ctggcagaat aattcgtatt caaaccacag attgacttcc gctgcgggcg cgagattctt
     6601 caatttttca aactctgcga caactttccc gaaatagctt tcttcgtttt cgccgtaagc
     6661 gcttttgata aatccggctc tgagcttcca gaaatcttcg agattttcgg ctcgcacatc
     6721 gccttcgacc agacattcgc ggcagacgac gcgttcgcct tcaatgccgg tattattgaa
     6781 atcatttgcc agtgcatcgc ccgctaaaac gtgaattttc ataacattaa accaatggcg
     6841 taaaacaagc ttaaaatcgc tccggcgtaa attccggcga gaatatcatc ggcgcagacg
     6901 ccgattccgg cgggcaggct ttgcagggaa tcaatcgggt acggcttcca gatgtcaaaa
     6961 agacgaaaca gcaaaaatcc cgccagaata tatttccacg aaatatcaaa cggaacgaaa
     7021 agaaaaacca aaagctgccc gatgacttca tcgacgaccg cttgcgacgg gtctttgtgt
     7081 ttgaaaagct tggtcgcccg cgaagccgcc cagattccaa gcaaacaaaa cagaagaaaa
     7141 agaatcgcgt tgaagacgtg aatccaggcg ataatttgtg cttcctgcca gccgcgcaaa
     7201 gctaaataag ccgcgccgct cgcttcgatt tgacaaaaac cgagataaat caaaacgccg
     7261 accgccgaac cccaggttcc cggcgcaagc ggaatataac cgactccaaa cgttgtgacg
     7321 gctaaagaaa aataatcgag aaaacttttt tctccgattt cttccttcgc cagttccgct
     7381 ctctcgaccg aggcggagat gtgtatcagt tcgtcgtaac ccggcattaa attaaaattc
     7441 catatgattt ctgcgcgttg cggcaatcta ctttcctatc gattatcacc ttcgatacgt
     7501 cagattctat tttatccggc agagcgggaa tgcagaatca gcctgtaatt ttattaaaaa
     7561 gcccgctaaa ataactcgga cgcatttggt ctgaaatatt atttcgagaa catcgcgtcg
     7621 atttccgcaa gcaaattcgc gaaggcttta ttaccgcttt cgtctttgtc gcgcgggcgc
     7681 gtggcgcgga ttttgataaa tctgttttga tgcgcaaaga tatagatttc cgaagttaga
     7741 ttccgcccgc ccgcgctgag attataaagc gcgcgcagag cttttatctt gccgctcgcg
     7801 ccgcccagcg ttacggtgtc gtttttaact tccttgacgt tttgataata acccatatcg
     7861 gctacctttt gaatttcgtt tctggcatta gcaaattcgt ttttaaggat tttatcgttt
     7921 aaatcattgg gaatgtttga cttgccgccg ttgtagacat aaatcgtgac cctgccgcct
     7981 tcttccgaca tatagttgac gctgaaaccg agttcttcgc tcggatattt ttgaatttcg
     8041 tctctctccc agccttcgac gtcggggaaa tcaattgcta aatctttaag cgtgaccggc
     8101 gct
//
LOCUS       DASWNE010000335         3596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_116414, whole genome shotgun sequence.
ACCESSION   DASWNE010000335 DASWNE010000000
VERSION     DASWNE010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116414"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="VGB68_06825"
     CDS             <1..989
                     /locus_tag="VGB68_06825"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HEY0048979.1"
                     /translation="EQYDHMVRTNTAILPGADAAVVRVKETRRAIAMCLDGNGKFVAV
                     NPREGAKLIVAEAARNNVCVGAKPIAVTNCLNFASPERPEVMWSFSEVVDGMKEACEA
                     FDTPIVSGNVSFYNETEGRGILPTPTIGMVGLIDDARKIITQGFKTPGDIIALLGETK
                     NDLAASEYEQIILGLTTDELIENGEVPALDLDLERRVQETCLKLADRFLINSAHDCSD
                     GGLATALAENCFSSLNRKATGAEIDLKDENLDAAAQLFGESPSRIIVGFAPEKLTEIE
                     ATAKENDCPFQIIGKVSGAELKIKFNGEQIVSAAVTELQRRWKTSLENQLED"
     gene            1002..2144
                     /gene="hemW"
                     /locus_tag="VGB68_06830"
     CDS             1002..2144
                     /gene="hemW"
                     /locus_tag="VGB68_06830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEY0048980.1"
                     /translation="MKAGIYLHIPFCRSRCSYCDFATDVFKNAETVERYVDALVSEIE
                     NSSRLPLYGSHPVDTIYFGGGTPSLLSPRQLEKILDSIYKRFPVEKNIELTMEMNPAT
                     VTLETLKEYRNLGVNRASFGAQTFDDAELKRLGRRHTAEDVRETIGLLRRAGFANVSF
                     DLIAGLPRQTLADWERNLDEALNLKPEHLSLYLLEIHEGTPLAEQIRSNRQPLPDEDL
                     AGEMYELMLDKTSAKGYAQYEISNFSLDGLESRHNSKYWLCEPVYAFGVSAHSFDGRR
                     TRWANERDTNRYVSLIENHQTPIAEKNELDAKQLAAEFAFLRLRLTRGLPLGEYKNRF
                     GIDLREKFAEDLRRLEDLDLIELDADFLKLTRKGFVFSNEVFALFV"
     gene            2236..3153
                     /locus_tag="VGB68_06835"
     CDS             2236..3153
                     /locus_tag="VGB68_06835"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0048981.1"
                     /translation="MKLHKNLKAVLLLVVVTMLASSAAFAALPEDGGVIDGKYSKNRE
                     AENLMKEAESYFSQGEFETALAAFGKALKLDPNIYEAALFSGEAYVQTGSYDDAEIWF
                     QKAIAIDPNREAAYRCSAEPLMQQERYELARDRYIEAYIVEPYSRFPVAGLIHWAQIT
                     NTGILHPAIDVPEIKYDEKGNAKSALNINPLLEDGSAAWTAYAAARESWRKDKFAKTF
                     PNQKTYRHSLQEEAEALRGVIRAFKEKKAKNPNEQLSLLSKLNDEGFLEPFILLAQPD
                     EGIAQDYAEYMKRNRDRLRQYVLKYVVKK"
     gene            3213..>3596
                     /locus_tag="VGB68_06840"
     CDS             3213..>3596
                     /locus_tag="VGB68_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048982.1"
                     /translation="MKNRIVKFLFAVSFLNVCFAFSPNAQIPKTNEAAAVPKRLPENN
                     AMETRQIDLKGCIQTSRGQQFVIKDNETYLKTIRGDASLDWCLKKLEKIDFGKHALVG
                     IEINSGYCRIPLGLRYQAYKDADKKQ"
BASE COUNT          952 a          871 c          953 g          820 t
ORIGIN      
        1 acgagcaata tgaccatatg gttcggacaa atacggcgat tttgcccggc gcggacgcgg
       61 cggtcgtgcg cgtcaaggaa acgcggcgcg ccatcgcgat gtgtctggac ggaaacggaa
      121 aattcgtcgc cgtaaatccg cgcgaaggag cgaaattaat cgtcgccgaa gccgcacgaa
      181 acaacgtttg cgtcggcgcc aaaccgattg ccgtaacgaa ttgtctgaat ttcgcctcgc
      241 ccgagcgtcc cgaagtgatg tggtcgtttt cggaagtcgt ggacggaatg aaagaagcct
      301 gcgaagcttt cgatacgccg attgtctccg gcaacgtttc tttttataac gaaaccgaag
      361 ggcgcggaat tttgccgacg ccgactatcg gtatggtcgg tttgattgac gatgcgcgaa
      421 aaatcatcac gcagggtttt aaaacgccgg gcgacattat cgcgctgctc ggcgaaacga
      481 aaaacgattt ggcggcgagc gaatacgaac agattatttt aggcttaacg accgacgagc
      541 ttatagagaa cggcgaagtt cccgcgctgg atttggattt ggaacggcgc gttcaggaaa
      601 cttgtctgaa gctcgccgac cgatttttga taaattcggc gcacgactgc tcggacggcg
      661 gcttggcgac ggcgcttgcc gaaaactgtt tttcttcatt gaatcgaaag gcgaccggcg
      721 cggaaattga tttgaaagac gaaaatctgg acgcggcggc tcagcttttc ggcgaatctc
      781 catcgagaat tattgtcggt tttgcgccgg agaaattgac ggaaatcgaa gcgacggcga
      841 aagaaaacga ttgtcctttt caaatcatcg gaaaagtttc gggcgcggag ctgaagataa
      901 aattcaacgg cgagcagatt gtttcggctg cggttacgga gcttcagagg cggtggaaaa
      961 cttcgctgga aaatcaattg gaagattagc gagcgtgtaa tatgaaagca ggcatttatc
     1021 ttcacattcc tttttgccgc tcgcgctgct cttactgcga ttttgcgacc gacgttttta
     1081 aaaacgcgga aacggtcgag cgttatgtcg acgcgctggt cagcgagatt gaaaactcct
     1141 cccggttgcc gctttacggc tcgcatccgg tcgacacgat ttatttcggc ggcggaacgc
     1201 cctcgctttt atcgcccagg cagcttgaaa aaattctcga ttcgatttat aaaaggtttc
     1261 cggtcgaaaa aaatatcgag ctgacaatgg aaatgaatcc ggcgaccgtg acgctcgaaa
     1321 cattgaaaga atacaggaat ttaggcgtca atcgcgccag tttcggcgcg cagacctttg
     1381 acgacgcgga actgaaaaga ttgggcagac gccacacggc tgaggacgtt cgggaaacca
     1441 tcgggctttt gcggcgcgcc ggtttcgcaa atgtcagctt tgatttaatc gccggattgc
     1501 cgcgccagac gctcgccgat tgggaacgga atctggacga ggctttaaat ttaaaaccgg
     1561 aacatttgtc gctttatctt ttggagattc acgaaggaac gccgctcgcc gagcagattc
     1621 gctcaaaccg acagcccttg ccggacgaag atttggcggg cgaaatgtat gaattgatgc
     1681 tcgacaaaac gtcggcgaaa ggctacgcgc aatacgagat ttccaatttt tcgctcgacg
     1741 gtttggagtc gcggcataat tcaaaatact ggctgtgcga gccggtttac gctttcggcg
     1801 tttcggcgca ttcatttgac ggcaggcgca cgcgctgggc aaacgagcgc gacaccaatc
     1861 gatatgtttc gctgattgaa aaccatcaaa cgccaatcgc ggaaaaaaac gagctcgacg
     1921 ccaaacagct cgccgccgaa ttcgcctttc tcagattgcg cctgaccaga ggcttgccgc
     1981 tcggcgagta caaaaatcgt ttcggcatcg atttacgcga aaaatttgcc gaagatttac
     2041 ggcggctgga agatttggac ttgattgagc tcgacgccga ctttttaaaa ctgacgcgca
     2101 aaggtttcgt tttttcgaat gaagttttcg ctttgtttgt ttaatcaaaa aatcaaaata
     2161 agcaaggcaa aattaatcgc ggctcttcgt caataactaa acgcctgaat aaggaataaa
     2221 tccgaagtga aaaatatgaa gttgcataaa aatctgaaag ccgtactgct tctggttgta
     2281 gtcacgatgc tcgcttcgtc cgccgctttc gccgcgctgc cggaagacgg cggcgttatc
     2341 gacggaaaat attccaaaaa ccgggaagcc gaaaatttga tgaaggaagc cgaatcatat
     2401 ttttcgcagg gcgaattcga aaccgcgctc gcggctttcg ggaaagcctt aaaattagac
     2461 ccgaacattt atgaagccgc gcttttttcc ggcgaggctt acgtgcagac gggcagttac
     2521 gacgacgcgg aaatctggtt tcaaaaggca atcgccatcg acccgaaccg cgaggcggct
     2581 taccgctgct cggcggagcc tttaatgcag caggaaagat acgaactggc gcgcgaccgt
     2641 tacatcgagg cgtatatcgt cgagccttac agccgctttc cggtcgccgg tttaatccat
     2701 tgggcgcaga ttacaaatac gggaatattg cacccggcaa tcgacgttcc cgaaattaaa
     2761 tacgacgaaa aaggcaacgc caaaagcgct ctgaatataa atccgcttct cgaagacggt
     2821 tccgcggcgt ggacggctta cgccgcggcg cgcgaaagct ggcgcaaaga taaatttgcc
     2881 aaaacctttc cgaatcagaa aacttatcgt cacagccttc aggaagaagc cgaagcgctg
     2941 cgcggcgtta tcagggcttt caaagagaaa aaagcgaaaa acccgaacga gcagctttct
     3001 cttttgtcga aactaaatga cgagggcttt ctcgagccgt ttattctgct ggcgcagccg
     3061 gacgaaggca tcgcgcagga ttatgccgaa tatatgaaac gaaaccgcga ccggctgcgg
     3121 caatatgttt tgaaatacgt cgttaaaaag tagatgccga aggcggagcc tgatgctgct
     3181 ccgctttttc ttttttgaat cgagaagttt ctatgaaaaa tcgtatcgtc aaattcctgt
     3241 ttgccgtttc gtttctgaat gtttgttttg cgttttcgcc caacgctcag attcccaaaa
     3301 cgaacgaagc ggcggcggtt ccgaagcgtc ttccggaaaa caatgcgatg gagacgcgcc
     3361 aaatcgattt gaaaggctgc attcagactt ctcgcggtca acaattcgtc attaaagaca
     3421 atgagactta cctgaaaaca atccgcggcg acgccagcct cgattggtgt ctgaaaaagc
     3481 tcgaaaaaat tgatttcggc aaacacgctc tggtcggcat cgagataaat tccggctatt
     3541 gccgcattcc gctcggtctt cgatatcaag cctacaaaga cgccgacaaa aaacaa
//
LOCUS       DASWNE010000336         2363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_362965, whole genome shotgun sequence.
ACCESSION   DASWNE010000336 DASWNE010000000
VERSION     DASWNE010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362965"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="VGB68_06845"
     CDS             <1..828
                     /locus_tag="VGB68_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY0048983.1"
                     /translation="DAPRMIGVYLDGDGPFGDSYQINSDNNGPYGDSLTQELIPQIER
                     RFRAVGKPEARFLTGGSTGGWVSLALQIFYPDYFNGAWSGFPDPLDFRAYQLIDIYGD
                     ENAYVNKNGFERPSAREANGDTKFTMRLECLKENVLGAGDTYTTAGGQWGAWNAVYSP
                     RGADNRPVPLWDAKTGKINRAVAEHWKKYDLRLVVQENWQTLAPKLKGKLHIWVGEAD
                     DYFLNNGVHLFDDFLTKAEPKYEGWIMYSRTGRHGWYPKTQIELMKEMAQRAEQSLK"
     gene            842..2146
                     /locus_tag="VGB68_06850"
     CDS             842..2146
                     /locus_tag="VGB68_06850"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY0048984.1"
                     /translation="MNPTTEPKQQSEIVRKHKEFLFPAVATYYEEPLALVRGEGEYVW
                     DDAGNRYLDCFGGVLTVSVGHANPKINAAIIEQVNKISHTSTLYANHPQGDLAEKLAS
                     ITPGDLKKSFFTSSGTEADDTAIMAAKLATGRHEIVVLRHSYSGRSATALSSVGHSTW
                     RPLPSQVAGIVHARAPYCYRCPLSLSPKNCGLACAEDVKDVIMTTTTGEIAAFMAEPI
                     LGVGGFIVPPAGYFERVEEITREHGGLFIADEVQTGWGRTGDKWFGIEHWDVKPDIIT
                     SAKGMGNGVPIGMTTATAAVADKYPGMTFATFGGNPVSSAAALAVIKLIEEEDLRTNA
                     RVVGDYFRGRLEELKEKYPIIGDVRGMGLMQGIELVKDRDTKEPAPQAVLKVFEETRR
                     QGVLIGKGGLYGNVIRTGLMLNSTRDHVDELVAALDKGFALC"
BASE COUNT          586 a          620 c          681 g          476 t
ORIGIN      
        1 gacgcgccgc gaatgatcgg cgtttatctg gacggcgacg gacctttcgg cgattcgtat
       61 caaatcaatt ccgacaacaa cggaccttac ggcgattcgt taacgcagga attgattccg
      121 caaatcgaaa ggcggtttcg cgccgtcgga aagccggaag ccagattcct gaccggcggc
      181 tcgacgggcg gctgggtttc gctggcgctg caaattttct atccggatta ttttaacggc
      241 gcctggtcgg gctttcccga cccgctcgat tttcgcgctt accagctgat cgatatttac
      301 ggggacgaaa acgcgtacgt taataaaaac ggttttgagc gcccgtcggc acgcgaagcg
      361 aacggcgata cgaaatttac gatgcgcctc gaatgcctga aagaaaacgt tctgggcgcg
      421 ggcgacacct acacgacggc gggcggtcaa tggggcgcgt ggaatgccgt ctacagcccg
      481 cgcggcgcgg acaatcgtcc cgtgcctctg tgggacgcga aaaccggcaa aattaaccgc
      541 gcggtcgccg agcattggaa aaaatatgac ctgcggctgg tcgtccagga aaattggcag
      601 accctcgcgc cgaagctcaa aggcaagctg cacatctggg tcggcgaagc ggacgattat
      661 tttttgaata acggcgttca tctgttcgat gattttctga cgaaagccga gccgaaatat
      721 gaaggctgga ttatgtacag ccgcaccggc aggcacggct ggtatccgaa aactcagatt
      781 gaattaatga aagaaatggc gcagagagct gaacaatcgc taaaataaaa aatataaaat
      841 tatgaatcca acaacagaac cgaaacaaca atccgaaatt gtcagaaaac acaaggaatt
      901 tctttttccg gcggtcgcca cttattacga ggaaccgctc gcgctggttc gaggcgaagg
      961 cgaatatgtc tgggacgacg cgggcaatcg ctatctggac tgcttcggcg gcgttctgac
     1021 cgtcagcgtc ggtcacgcga atccgaaaat caacgcggcg attatcgagc aggtcaacaa
     1081 aatttcgcac acctcgacgc tctacgccaa ccacccgcag ggcgatttgg cggaaaagct
     1141 cgcctcgata acgcccggcg atttgaagaa atcttttttc accagcagcg gaaccgaagc
     1201 cgacgatacg gcgattatgg cggcgaagct ggcgaccggc agacacgaaa tcgtcgtttt
     1261 gcgccacagc tactcgggac gctcggcgac cgcgctatcg agcgtcgggc attcgacgtg
     1321 gcgaccgctg ccgtcgcagg ttgccggaat cgtccacgcc cgcgcgcctt actgttaccg
     1381 ctgcccgctc agcctgtcgc cgaaaaattg cggtctggcg tgtgccgaag acgttaaaga
     1441 cgtgattatg acgacgacga ccggcgaaat cgccgctttt atggctgaac cgattttagg
     1501 cgtcggcggt tttatcgtgc cgcccgccgg ttattttgag cgcgtcgagg aaatcacgcg
     1561 cgagcacggc gggcttttta tcgccgacga ggttcagacc ggctggggca gaaccggcga
     1621 caaatggttc ggcatcgagc attgggacgt caagcccgac atcatcacct cggcgaaagg
     1681 aatgggcaac ggcgtgccga tcggaatgac gaccgccacc gccgcggtcg ccgacaaata
     1741 tcccggaatg actttcgcca ccttcggcgg aaatccggtt tcgtcagccg ccgcgctcgc
     1801 cgtcatcaaa ttaatcgaag aagaagattt gcggacgaac gcgcgcgtcg tcggcgatta
     1861 ttttcgcggc aggctcgaag aattaaaaga aaaatatcca atcatcggcg acgttcgcgg
     1921 aatgggcttg atgcagggca tcgaactcgt caaagaccgc gacacgaaag agccggcgcc
     1981 gcaagccgtc ctgaaagttt ttgaagaaac ccgccggcaa ggcgttctaa tcggcaaagg
     2041 cgggctttac ggcaacgtga ttcggacggg cttgatgctc aattcgacgc gcgaccacgt
     2101 tgacgagctt gtcgccgctc tggataaagg tttcgcgttg tgctaggtga ataaaattta
     2161 gggaagtttt ttttcaaaaa ataaaaataa caggagcatc tgatgaataa acagttgttg
     2221 atcaaggtag gttttgtatt aatggcagcg gtttcgggat ttgtcttttc agcttgcgac
     2281 agttcttccg cgccttcgcc gccgaaagaa ttgaccgagg aagaaaaaaa ggcgacggaa
     2341 gggcgcgaag ctttagcgac gaa
//
LOCUS       DASWNE010000337         2432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_541010, whole genome shotgun sequence.
ACCESSION   DASWNE010000337 DASWNE010000000
VERSION     DASWNE010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541010"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..632
                     /locus_tag="VGB68_06855"
     CDS             84..632
                     /locus_tag="VGB68_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048985.1"
                     /translation="MLTTFLIIGGIGFLFLLVSLVVGDIFDAFGLDFGLDASHDFGIF
                     DSRVISVFLVAFGGFGVIGSQVGFGALGSTLFGLGGGLAFGTIVFFFGKFLYGQQSTS
                     SVRAEDLIGRTAQVVVNIKPDQLGQISCRIGDEKIEKLARAVGGEEIKAGQLVRIDSI
                     GSDSVIVSVDAGEGFSLFSEKA"
     gene            772..2406
                     /locus_tag="VGB68_06860"
     CDS             772..2406
                     /locus_tag="VGB68_06860"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY0048986.1"
                     /translation="MFELPIIVIAVVVAVVVSLLIAMMLLSKNYIKVSPNQAAVISGR
                     RRKLSDGSTVGYRQVRGGATLVIPFLEKVEFLDLNVITVPLATSRAYTSQGVPVSVKA
                     VANVKIKGDDESLRAAAERFLGMPKDQFHGLIFQTLEGHLRAILGTLTVEEINNDRQS
                     FAQKLTSEAAGDLEKMGIGLDALTIQEISDDEGYLDALGKRRTAEVKRDAEIGQAEAN
                     RDSKIKASLALQEGEKVRLSTEALIAQSNRETEIQKAQVSAEIQKERAKANQAGPLAE
                     AKAQQEVVAEEVRIERIRTQEQIAVQEQEVLRKEKELEATVVKQAEADRRAAVLRAQG
                     AQESAILEAEGRKQAQIAQAEAEAQKLQKEGQGRAMAIESEGRAEAEKIRALGLAEAE
                     KLQAQGLAHAKATEAQGLAEATAIKEKAAAWREFNDAARLQTILEKLPSIIEASAPVF
                     QAVAEPLGKIDKIVMIDQGGNGNGSSGINRFAQTAPTMIFSLLQQLQALGLNVPDVLA
                     QLGVNTDGKLTNGTEKTEIKLVEEIKAVEDGNGKQI"
BASE COUNT          600 a          644 c          704 g          484 t
ORIGIN      
        1 aatgattttc gttttaatat aaaagcgcaa agcttctgaa acgcttgcgc cgatgcgggg
       61 caaaaaccgt gactgatatt actttgctaa ctacttttct gataatcggc ggcatcggat
      121 ttctatttct gctggtctcg ctggtcgtcg gcgatatttt cgacgcgttc gggctggatt
      181 tcggcttgga cgcgagccac gatttcggca ttttcgacag ccgcgttatc agcgtttttc
      241 tggtcgcttt cggcggattc ggagttatcg gctcacaggt cgggttcggc gcgctcggca
      301 gcacgctgtt cggcttgggc ggcggcttgg cgttcggcac gattgttttt ttcttcggga
      361 aatttcttta cggtcagcaa tcgaccagct ccgtccgcgc ggaagactta atcgggcgca
      421 cggctcaggt cgtcgtcaat atcaagcccg accagctggg acagatttcc tgccgcatcg
      481 gcgatgaaaa aatcgaaaaa ctggcgcgcg ccgtcggcgg cgaagaaata aaagccggtc
      541 agctcgtccg catcgattca atcgggagcg attcggttat cgtcagcgtc gacgcgggcg
      601 aaggcttttc gcttttttcc gaaaaggctt gaaacaaacc cgttttcaga ttaaaattcg
      661 cacaacccaa aatcaaaata tcaaattttg gtttccttat cgcacgagtt tcgctatttt
      721 cgcgtgtcaa ttttccggct tgccaatcgg caggcgtaaa gtgaggtaaa aatgtttgaa
      781 cttcctatta tcgtaatcgc agtggttgtc gctgtggtcg tcagtctgct catcgcgatg
      841 atgctcttga gcaaaaacta catcaaggtt tcgccgaatc aggcggcggt tatttccggt
      901 cgccgccgca aattaagcga cggctcgacg gtcgggtatc gacaggtgcg cggcggcgcg
      961 acgctcgtca ttccgtttct ggaaaaggtc gaatttctcg atttaaacgt tatcaccgtg
     1021 ccgctcgcca cgtcgcgcgc ctacacttcg cagggcgttc cggtttcggt caaagccgtc
     1081 gccaacgtca aaatcaaagg cgacgacgaa tccctgcgcg cggcggcgga gcgatttctg
     1141 ggaatgccga aagaccagtt tcacgggctg attttccaga cgctggaagg tcacctgcgg
     1201 gcgattctcg gaactctgac ggtcgaagaa atcaacaacg atcgccagag cttcgcgcag
     1261 aaactgactt ccgaagcggc gggcgatttg gaaaaaatgg gcatcgggct cgacgcgctg
     1321 acgattcagg aaatctccga cgacgaaggt tatctggacg cgctcggcaa acgccgcacg
     1381 gcggaagtca agcgcgacgc cgaaatcggg caggcggaag ccaaccgcga ttcgaaaatc
     1441 aaggcgtctt tagctttgca ggaaggcgaa aaagttcgat tgagcaccga agctttaatc
     1501 gcgcaatcga accgcgagac cgagattcaa aaagcgcagg tctcggcgga aatccagaaa
     1561 gaacgcgcca aggcaaatca agccggaccg ctggcggaag ccaaagcgca acaagaggtg
     1621 gttgccgaag aagttcgtat cgagagaatt cgcacgcagg aacagattgc ggtgcaggaa
     1681 caggaagttc tgcgcaagga aaaggaactc gaagcgacgg tcgtcaaaca agccgaagcc
     1741 gaccgccgcg ccgccgttct gcgcgctcag ggcgcgcagg aatcggcgat tttagaagcc
     1801 gaaggtcgca agcaggcgca aatcgcgcaa gccgaagccg aagcgcagaa attgcagaaa
     1861 gaaggtcagg ggcgcgcgat ggcaatcgaa tcggaaggtc gcgccgaagc cgaaaaaatc
     1921 cgcgcgctcg gtttagccga agccgaaaaa ttacaggcgc agggtttggc gcacgcgaaa
     1981 gccaccgaag cgcagggctt ggcggaagcg accgccatca aggaaaaagc cgccgcctgg
     2041 cgcgaattta acgacgccgc gcgcctccaa acgattctcg aaaaactgcc ttcgataatc
     2101 gaagcttccg cgccggtctt tcaggcggtc gccgagccgc tcggtaaaat agacaagatt
     2161 gtgatgatag accagggcgg caacggaaac ggctcaagcg gaatcaatcg cttcgcgcaa
     2221 accgcgccga cgatgatttt ctcgctgctc caacagcttc aggcgctcgg cttaaacgtc
     2281 ccggacgtgc tcgcgcagct cggcgtcaat acggacggca agctgacgaa cggcacggag
     2341 aaaaccgaaa tcaagctggt cgaagagata aaagcggtcg aggacggcaa cggaaagcaa
     2401 atttaatcgc ggtccattca accttttcgc aa
//
LOCUS       DASWNE010000338         2878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_646472, whole genome shotgun sequence.
ACCESSION   DASWNE010000338 DASWNE010000000
VERSION     DASWNE010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646472"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(269..1462)
                     /locus_tag="VGB68_06865"
     CDS             complement(269..1462)
                     /locus_tag="VGB68_06865"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HEY0048987.1"
                     /translation="MSEQNTFQDNFQEILKTLEKNATEFFEGVADTFVGKADTLRDGT
                     EYDVVQLPRDLAAHKNVQTEWWYYTGHCETEAGNRFGFELVFFKRRTDLDKFSVVPLR
                     LLGNPIFFAHFAITEYENKQFRYAHRKSSNGWFDFPASASERHFHLRLGDWSLREAKG
                     AHILRATIGDIVFEATLKPAKKVVLNGKEQNGVSFKDKGEASRYFSYTRMETEGDIIW
                     NGETEHFKGSAWMDREFGTWTPTENQKGWDWFSIQLEDNRELMCYQLRNSADGISEYS
                     SGSFVDAEGNSTFLAHEDFSIEPTGFWKSPRTEATYPSGWRVKVPRYDLDLTVTPVAD
                     DQELDTRGTTMIVYWEGACEVTGENTGGRAYVELVGYDRSHESPNLASFLMGNSFEFP
                     SKSYF"
     gene            complement(1802..2644)
                     /locus_tag="VGB68_06870"
     CDS             complement(1802..2644)
                     /locus_tag="VGB68_06870"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY0048988.1"
                     /translation="MSCKDLQEAIALYADGDTLEENARARLDAHLVACPPCRVRVAEF
                     QTLQNDLRVLSRPAMPADLLNSVRNAVAVEIRTAERQQPFIYSESFRRWLQFRFMPYA
                     VGTVASLVLTCTLLLSLLSSRNLTIENNLAATDSGSASSQILLASNSNPMIDELKITP
                     SEYAAMRLPVANESPSVNPTGALVALTKSIVRGKMRDDEVVVVADVFSNGVARISEVI
                     EAPSNDNDLEALENALQKDPNEAAFLPARLDNRSDVVRVVLKIQRVDVIDKARTPKNK
                     PKNR"
     gene            complement(2699..>2878)
                     /locus_tag="VGB68_06875"
     CDS             complement(2699..>2878)
                     /locus_tag="VGB68_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048989.1"
                     /translation="QQKSCRQPEKQSRKIAPSKRVVPGASRKQPGSVSHSLELVIRQL
                     SVAGMLFECEIHSEF"
BASE COUNT          688 a          738 c          713 g          739 t
ORIGIN      
        1 taggaaatgt gtcgttaatc aatgttttgc attttaattt ataaatgatt ccgtgaaaaa
       61 aaacaagcta atttgttttc aaccgcgaaa tttaactttt cggaaaagtt ttagactgct
      121 ttgaattaat tccaatctct aagacgtgag agaaatcgct ttggtcgaaa aaaatttact
      181 gtctgtggta aatggtaaac ggtaagttgc aatcaataag cactgagttg caaacaaaaa
      241 gtaaaaatta caacttaccg cttgcgggtt aaaagtagga tttgctcgga aactcgaaag
      301 aatttcccat caggaaagac gccagattgg ggctttcgtg cgagcggtcg taaccgacca
      361 gttcgacgta tgcgcgaccg cccgtgtttt ctcccgtcac ctcgcacgcg ccttcccagt
      421 aaacaatcat cgtcgtgccg cgcgtgtcca gctcctgatc gtccgcgacg ggcgtgacag
      481 ttaaatccaa atcgtaacgc ggcactttga cgcgccagcc gctcggataa gtcgcttccg
      541 tgcgcggcga tttccagaag ccggtcggct cgatcgaaaa atcttcgtgc gccagaaaag
      601 tcgagtttcc ttccgcgtcg acaaagctgc cgctcgaata ttccgagatt ccgtcggcgc
      661 tgttgcgaag ctggtaacac atcaattcgc gattatcttc gagctgaatc gaaaaccaat
      721 cccagccttt ctgattttcg gtcggcgtcc aggtgccgaa ttctctgtcc atccacgccg
      781 agcctttaaa atgctcggtt tcgccgttcc agatgatgtc gccctcggtt tccatccgcg
      841 tgtatgaaaa atagcgcgaa gcctcgcctt tgtctttaaa ggaaacgccg ttttgttctt
      901 tgccgtttaa aacgaccttt ttcgcgggtt tcagagtcgc ttcgaaaacg atgtcgccga
      961 tggtcgcccg caaaatgtgc gcgcctttcg cctcgcgcag agaccagtcg cccagacgca
     1021 gatgaaaatg ccgctcgctc gccgacgccg gaaaatcgaa ccagccgttc gagcttttgc
     1081 ggtgcgcgta gcgaaactgc ttgttttcgt attcggtaat cgcaaaatgc gcgaaaaaaa
     1141 tcggatttcc caaaagccgc agcgggacga cgctgaattt gtccaaatcc gtgcggcgtt
     1201 taaaaaagac gagctcaaag ccgaagcgat ttcccgcttc cgtctcgcaa tgcccggtgt
     1261 aataccacca ttcggtctgc acgtttttgt gcgcggcaag gtcgcgcggc aattgaacga
     1321 cgtcgtattc cgtgccgtcg cgcagcgtgt cggcttttcc gacaaaagta tcggcgacgc
     1381 cttcaaaaaa ctcggtcgcg tttttttcta aagtttttaa gatttcctga aaattatcct
     1441 gaaaggtatt ttgttcgctc atatataaat tgtagcaaaa ttgcttacgg aaagacgaaa
     1501 cggcggttcg aatgattttc tttgacccgc cgaaatttcg atgcgtttgc cgagaaaatt
     1561 atccgcccgt tcagacttct aaaatgcccg tcggcaaaat tctttgctca tcgaaacttt
     1621 gatggcgctc attctcatta aaattcgctc atcgtaaaaa aatattgctc attctgatgt
     1681 ttcgagcgct cgccggagtt ttaaaagagc atgcggcaaa ttgtacggat taatgaaaaa
     1741 accgctccgt aaagcttacg aagcggtttc gacgggtttt gtaaaagcgg tgtgaggaat
     1801 atcagcggtt tttaggcttg ttttttggcg tgcgagcttt atcaatcacg tcgacgcgct
     1861 gaattttcag cacgacgcgg acgacgtccg agcgattgtc caatcgggcg ggcagaaaag
     1921 cggcttcgtt cgggtctttt tgcagagcgt tttcgagcgc ctccaaatcg ttgtcgttgc
     1981 tcggcgcttc gataacttcg gagattcgcg ccacaccgtt gctgaaaacg tcggcgacca
     2041 cgaccacttc gtcatcgcgc atcttgccgc gcacgattga tttggttaaa gccaccagcg
     2101 cgccggtcgg attgaccgaa ggcgattcgt tggcgaccgg cagacgcatc gcggcatatt
     2161 cggacggcgt aattttcagc tcgtcaatca tcggattgct gttcgaagcc agcagaatct
     2221 gcgatgaggc ggaaccggaa tccgtcgccg ccagattatt ttcaatcgtt aaatttctgc
     2281 tcgaaagcag gctcagcagc agcgtacacg tcaaaaccaa cgaagccacc gtgccgaccg
     2341 catacggcat aaagcgaaat tgcagccagc ggcgaaagct ttccgagtaa ataaaaggct
     2401 gttgtctttc cgccgttcta atttcgacgg cgaccgcatt tctcaccgaa tttaataaat
     2461 cggcgggcat cgcgggacgc gacaagacgc gcaaatcgtt ttgcaatgtt tgaaattcgg
     2521 caacccgcac gcggcacggc gggcacgcga ccagatgcgc gtccagacgg gcgcgagcat
     2581 tttcttccag cgtgtcgccg tcggcgtaaa gcgcgattgc ttcttgtaaa tctttgcaag
     2641 acataatttt ttcgtccttt gtccgttgtg ggtcgccggt cgcccgtcgc ggcttgaatc
     2701 aaaattcaga atgtatttcg cattcaaaca gcataccggc aactgacaac tgacgaatga
     2761 ccaattcgag cgaatgactg accgaccccg gctgtttccg gcttgcgccg ggaacaactc
     2821 ttttgctcgg tgcgattttt cgggattgct tttccggctg gcggcaactt ttttgttg
//
LOCUS       DASWNE010000339         2292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_258844, whole genome shotgun sequence.
ACCESSION   DASWNE010000339 DASWNE010000000
VERSION     DASWNE010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258844"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1786
                     /locus_tag="VGB68_06880"
     CDS             <1..1786
                     /locus_tag="VGB68_06880"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEY0048990.1"
                     /translation="HVDLDWDVLFDRKVLRGTATLRLERGSNGENAPLILDTRDLNIE
                     KVEASNDGVRYAAIGFHLGTADKILGAPLTILLPERANRVRIHYSTSPAASGLQWLEP
                     QQTAGKKTPFMFSQAQAIHARSFIPIQDSPQVRLTYTARVRTPKNLLAVMSADGNSQT
                     AARGGDYRFRMTHPIPSYLIAIAVGDLQFRSLGKRTGVYTEPATLAKAAQELSDTEKM
                     VEATEKTYGAYRWGRYDLLVLPPSFPFGGMENPMLTFATPTILAGDKSLVSLVAHELA
                     HSWSGNLVTNATWRDFWLNEGFTTYLERRIIEEVYGKPRREMEATLGRRSLEEEMSGM
                     EARDQILHVDLQGRDPDEGFTGVPYEKGALFLRHLEEKVGREKFDKFVRGYFDEFAFQ
                     SITTETFVDYLQKNLIDKQNPSKNYTMATIMSMVKREDVDEWIEKPGLPKNAPQPASS
                     AFRKVETQAQEWLGGNLKTSEIQARAWTTQEWLHFLKSLPPDVGAQKMAELDRAFSLT
                     RSGNSEISFQWLLMSIRNKYEAANPRLEEFLTSIGRRKFVKPLFEELVKTPEGKTRAG
                     SIYAKARPGYHPITQAAVDAILNWKGQP"
     gene            1836..2216
                     /locus_tag="VGB68_06885"
     CDS             1836..2216
                     /locus_tag="VGB68_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048991.1"
                     /translation="MRERIKKTLYLFGGLSLPANLAALHALIILAAMAFYPTDIFIPD
                     APYDNVYIIYVLVPGIHILMIATALSQLLFPWLLTIMPPRAASVLCVVFIPGSVGILL
                     GGWQWYFIGKIILLFKRARQTELR"
BASE COUNT          544 a          574 c          636 g          538 t
ORIGIN      
        1 tcacgttgat ttggactggg atgttttgtt tgaccgaaag gttttgcgcg gaacagcgac
       61 tttgcgttta gagcgcggct cgaacggaga aaatgctcca ctcattctcg atacgcgcga
      121 tctaaatatt gaaaaagttg aagcgtcaaa cgacggagtg agatacgcgg cgataggatt
      181 tcatctcggc acggcggata aaattttagg cgcgccgctc acgattttat tgcctgagcg
      241 tgcaaaccgc gtacgcattc attattcgac cagcccggcg gcttccggct tgcagtggct
      301 tgagccgcag cagacggcgg gcaaaaaaac gccgtttatg ttttcgcagg cgcaggcgat
      361 tcacgcgcgc agctttatcc cgattcagga ttcgccgcag gttcgcctga cttatacggc
      421 tcgcgttcgg acgccgaaaa acctgctcgc cgtgatgagc gccgacggca attcgcaaac
      481 tgccgcgcgc ggcggcgatt accgttttcg gatgacgcac ccgattccgt cttacctgat
      541 tgccattgcc gtcggcgatt tgcagtttcg ctcgctcgga aaacgcaccg gcgtttacac
      601 cgagcccgcg acgctcgcga aagcggcaca ggaactttcc gatacggaaa aaatggtcga
      661 ggcgaccgaa aaaacttacg gcgcgtatcg ctggggacgt tacgatttgc tggttctgcc
      721 gccgagcttt ccctttggcg gaatggaaaa tccgatgctg acctttgcca cgccgacgat
      781 tctggcgggc gataaaagtc tggtctcgct cgtcgcccac gagctggcgc attcgtggtc
      841 gggcaatctc gtgacgaacg cgacgtggcg cgatttctgg ctcaacgaag gtttcacgac
      901 ttatctggaa cggcgaatta tcgaggaagt ttacggcaag ccgcgccgcg agatggaagc
      961 gactttggga cgccgctcgc tcgaagaaga aatgtccgga atggaagcgc gcgaccagat
     1021 tctgcacgtc gatttacagg ggcgcgaccc ggacgaaggt tttacgggcg tgccgtatga
     1081 aaaaggcgcg ctttttctgc gtcatctcga agaaaaggtc gggcgcgaaa agtttgataa
     1141 attcgtgcgc gggtatttcg atgaatttgc ttttcagagc atcacgaccg aaactttcgt
     1201 cgattattta cagaagaatt taatcgacaa acaaaaccct tccaaaaatt acacgatggc
     1261 aactattatg tcgatggtaa aacgcgagga tgttgacgag tggatcgaaa aacccggttt
     1321 gccgaaaaac gcgccgcagc ctgcttcgag cgctttccgc aaagtcgaaa cgcaggcgca
     1381 ggaatggctc ggcggaaacc tgaagacgag cgagattcag gcgcgcgcgt ggacgacgca
     1441 ggaatggctg cattttctga aaagtctccc gccggacgtc ggcgcgcaaa aaatggcgga
     1501 actcgaccgc gcgtttagcc tgacgcgcag cggcaattcg gaaatctcgt ttcaatggct
     1561 tctgatgtcc attcgcaaca aatacgaagc ggcaaacccg cggctcgaag agtttctgac
     1621 ttcaatcggg cggcgcaaat tcgtcaagcc gctgtttgaa gagctcgtta aaacgcccga
     1681 aggaaaaacg cgcgcgggca gcatctacgc caaagcgcgt cccggttatc atccgataac
     1741 gcaggcggcg gtcgatgcga ttttgaattg gaaaggtcag ccctgaaaat attcctttaa
     1801 gcggcaggaa aaagctccgg ctcatttctg cgaatatgcg tgagcgaatt aaaaaaactt
     1861 tatatttgtt tggcggctta tcgcttcccg ccaatctcgc ggcgcttcac gccttgataa
     1921 ttttggcggc gatggctttc tacccgacgg atatatttat tcccgatgcg ccttacgata
     1981 atgtttatat tatttacgtg ctggtgccgg gaattcatat tcttatgatt gcgacggcgt
     2041 tgtcgcaatt gttgtttccg tggcttttga cgataatgcc gccgcgcgcg gcgtccgttc
     2101 tgtgcgtcgt ttttattccc ggctcggtcg gaattctgct cggcggctgg cagtggtatt
     2161 ttatcgggaa aataattctt ttatttaaac gcgcgcgtca aactgaatta agataattgc
     2221 cgaaggcaaa aagcgatttt tgagacgcat ttatgaacaa aaaaataatt aaatcaattc
     2281 tgccggtgac gg
//
LOCUS       DASWNE010000340         3290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_116588, whole genome shotgun sequence.
ACCESSION   DASWNE010000340 DASWNE010000000
VERSION     DASWNE010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116588"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..1013
                     /locus_tag="VGB68_06890"
     CDS             219..1013
                     /locus_tag="VGB68_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY0048992.1"
                     /translation="MNFETVNYELNETVAIVTMNRPEALNALSLQLTRDLDAAFRRAV
                     EDKARAIVLTGAGRAFCSGGDLREMQSMWQREGRIEAFMEEPLKALHDLVLLIRETPV
                     PFVAAVQGVCAGAGTNFALACDIVFAAENASFNEAFVRIGLSPDCGGSFFLPRVVGEK
                     LAAEIFMTGETISAEKALAIGMINRVVTADNLMSEALAMAERLALAPTGAIGRIKRML
                     NASFSNDLSQQLSLEHESQIESGKSEDFKEGVTAFFEKRQPQFTGT"
     gene            1200..2471
                     /locus_tag="VGB68_06895"
     CDS             1200..2471
                     /locus_tag="VGB68_06895"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0048993.1"
                     /translation="MNLTYAQLLKGNRDFRNLFWGQIISELGNWFNFIAGLGLIRVVS
                     DSSPAAAGIFVVCRTLPFSLLMPLAGTFVDRFSRRQVMIWADLARVLVALMFLLVTKP
                     ENLWIAYLATTLLSAGGAFFEAAKNAATPNISGKEGLFAGTALMFTSRFLLMAIGSAL
                     AGLAAAYFGYQAAFVINAVSFLVSAFSIWLIPEEAVRENETAKKERDSFFAELKEGMF
                     YFWREPFVMTIVLMNIIWATGGGAINIVFERLGGVAFAQRESWNPDLAVALLWVASGL
                     GLAFGMLAAHRVNSIVERKNITVGFIGWSLVVHGILFSAAGFMPTLWLVLFFTFISRL
                     IVGVEYAVQETMLQRALPDYIRGRILTIDRGAEITMFSISGYFAGVSQYYISPETLAI
                     ISGVLSASAGVLWFMRSSKSKVRNAETAAEA"
     gene            complement(2665..>3290)
                     /locus_tag="VGB68_06900"
     CDS             complement(2665..>3290)
                     /locus_tag="VGB68_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048994.1"
                     /translation="TDSWWFDNVRQNIERLPSLFTKATAMTLAMQAGNYLLSFTEETR
                     ELRQPLSTVFRRLRSAYPKPVNNGQNNACHNKNAVEFIAENYTDLMFLRLPRAHNLSL
                     RNAMGWTAWQEEWLQAGDAFWTDLETAQTGKLGTHVETKNQYLRLVEDLLKTAAHIGK
                     WAIAHTEDGFISTHELVEIVGRVRQVDTIFTKDFSELTGMKAVIITA"
BASE COUNT          674 a          800 c          945 g          871 t
ORIGIN      
        1 atctgcgacc attgcggcaa gcacgaccac gtgccgtttc agccgaaggt cgggcgcagc
       61 gttttatgca aggaatgtca tcaggcgagc aaggcgcgaa tgcgctccgc ttaaaaaaag
      121 tgaaagagtg aaacagtgaa agagtgaaaa agcaggtgga acagtaatca tctaaacttt
      181 ttcgcatttt cacgttttca ctctttcact ttacttttat gaattttgaa actgtcaatt
      241 acgagctgaa cgaaaccgtc gcaatcgtta cgatgaatcg ccccgaagcg ctcaacgcgc
      301 tttcgctgca gctgacgcga gatttggacg cggcgtttcg gcgcgcggtc gaagataagg
      361 cgcgggcaat cgtcctgacc ggcgcgggac gcgcgttttg ttcaggcggc gatttgcgcg
      421 agatgcagtc gatgtggcag cgcgaaggca gaatcgaggc gtttatggaa gagcctttaa
      481 aggcgctgca cgaccttgtt ttgctgattc gggaaacgcc ggttcctttt gtcgcggctg
      541 ttcagggcgt ctgcgcgggc gcgggaacga atttcgctct cgcctgcgat atcgttttcg
      601 cggcggaaaa cgccagcttt aacgaagcgt ttgtccgcat cggactttcg cccgattgcg
      661 gcggcagctt ttttctgccg cgcgtcgtcg gcgaaaagct cgcggcggaa atttttatga
      721 cgggtgaaac aatttcggcg gaaaaagcgt tagcaatcgg tatgattaac cgcgtcgtga
      781 cggcggacaa tttaatgagc gaagcgttgg cgatggcgga gcggctcgcg ctggcgccga
      841 ccggcgctat cgggcgcatc aagcgaatgc ttaacgcttc gttttcaaac gatttgtcgc
      901 agcagcttag cctcgagcac gaaagccaga ttgaatcggg caaatcagag gattttaaag
      961 aaggcgtcac ggcatttttc gaaaagcgcc agccgcagtt taccggcact tgaaaaatta
     1021 ttttatggtt tgggcaaaag ccgtcaggtt tcgagcctgg cggctttttt tagttgacag
     1081 gaagttgaca gaaagttgca ggaagctgca ggaagttaga agaaattcta cattctgcaa
     1141 tctcttgtaa ctacctgcaa ctatccgcaa cttcctgcaa ctttctgcaa cttagagtta
     1201 tgaatttaac atacgcgcaa ttattaaaag gcaatcgcga ttttcgcaat ttgttttggg
     1261 gacagataat ttccgagctc ggaaactggt ttaactttat tgcgggtttg ggacttattc
     1321 gcgtggtttc ggattcttct ccggcggcgg cgggaatttt tgtcgtctgc cggacgctgc
     1381 cgttttcctt gctgatgccg ctggcgggaa cttttgtcga ccgtttttct cgccggcagg
     1441 tgatgatttg ggcggatttg gcgcgcgttc tcgtagcgct gatgtttctg ctggtcacaa
     1501 agccggaaaa cctctggatt gcgtatctcg ccacgactct gctttcggcg ggcggcgcgt
     1561 tttttgaagc ggcgaaaaat gccgcgacgc cgaatatttc cggcaaggaa ggacttttcg
     1621 ccggcacggc tctgatgttc acgtcgcgct ttctgctgat ggcaatcggc tcggcgctcg
     1681 ccggtttggc ggcggcgtat ttcggttatc aggcggcgtt cgtcatcaac gcggtttcat
     1741 ttctggtttc ggctttttcc atctggctga ttccggaaga agccgtgcgc gaaaacgaga
     1801 cggcgaaaaa agaacgcgat tcctttttcg ccgaattgaa ggaaggaatg ttttatttct
     1861 ggcgcgagcc gtttgtgatg acgattgtct tgatgaacat catctgggcg acgggcggcg
     1921 gcgcgattaa tatcgttttc gagcgtctgg gcggcgtcgc ttttgcccag cgggaatcgt
     1981 ggaatccgga tttggcggtc gcgcttttgt gggtggcgag cggtttgggt ctggctttcg
     2041 gaatgctggc ggcgcaccgc gtcaattcga ttgtcgagcg gaaaaacatc acggtcggct
     2101 ttatcggctg gagtctggtc gttcacggca tattgttttc ggcggcgggt tttatgccga
     2161 ctttgtggct ggttttgttt tttacgttta tctcgcgtct gattgtcggc gtcgaatacg
     2221 ccgtgcagga aacgatgctg cagcgcgcgc tgcccgatta tattcgcggg cggattctga
     2281 cgatcgaccg cggcgcggag attacgatgt tcagcatttc cggctatttc gcgggcgttt
     2341 cgcagtatta tatctcgccc gaaacgctgg cgattatctc cggcgtgctt tcggcgagcg
     2401 cgggcgtttt gtggtttatg cgaagctcga aatcgaaagt gcgaaacgcg gaaacggcag
     2461 cggaagcgta agaaaattta gccgccgacg ggtcagagat ttttgagaga agtcaaccac
     2521 ggattttcac agattaactt agctaagaaa tggatttatt atcctgaaat cctgttttaa
     2581 ttatcttgtg tttatctgtg aaaatccgtg gctgactctc cttttctccg tgttctctgc
     2641 ggctgatttt tcttcatagc tgatttatgc cgtaataatt accgctttca ttccagtcaa
     2701 ttccgaaaaa tcttttgtga aaatcgtatc tacctgacgg acgcgaccga cgatttcgac
     2761 cagttcgtgc gtcgagatga aaccgtcttc ggtgtgcgcg atcgcccatt tcccgatgtg
     2821 cgcggcggtt ttcagcaaat cttcgaccag ccgcagatat tgattttttg tttcgacgtg
     2881 cgttccgagc tttccggtct gcgccgtttc caaatccgtc cagaacgcgt cgcccgcctg
     2941 cagccattct tcctgccagg cagtccagcc catcgcgttg cgcaggctca gattatgcgc
     3001 gcgcggcagt cgcagaaaca tcaaatccgt gtagttttcg gcgataaact cgaccgcgtt
     3061 tttgttgtga caggcgttgt tctgaccatt gttgacgggc ttgggatacg cggaacggag
     3121 ccggcgaaag acggtcgaga gcggctggcg aagctcgcgc gtctcctccg tgaaagacaa
     3181 aagataattg cccgcctgca tcgccagcgt catcgccgtc gccttggtaa acagcgacgg
     3241 cagccgctcg atattctggc gcacgttgtc gaaccaccac gaatcggtct
//
LOCUS       DASWNE010000341         3395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_681090, whole genome shotgun sequence.
ACCESSION   DASWNE010000341 DASWNE010000000
VERSION     DASWNE010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681090"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..500)
                     /locus_tag="VGB68_06905"
     CDS             complement(33..500)
                     /locus_tag="VGB68_06905"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0048995.1"
                     /translation="MTAFKKSLLIRPLQPADAAAVSDFMRSQPPEYSRFFYAFGSDEA
                     MIAEILSAYRADVYSGVFWQENLVGIFMLRGWDAGFEVPSFGVLIDEKYRGGAFMRLT
                     LDTAKLICRLSGAKRLMAKIHPDNVSPRGASRLGLRQTGVEEATGNIIYHLEL"
     gene            complement(513..1322)
                     /locus_tag="VGB68_06910"
     CDS             complement(513..1322)
                     /locus_tag="VGB68_06910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0048996.1"
                     /translation="MASAAEANGSRKIFSIVLATYNCGRKVERTIESIICQNKDLFEL
                     IVVDGASTDDTLDYIKKYESDLRLISEKDGGVYDAFNKGIDLATGQYLYFIGAGDKLR
                     EGVLETVGEVLPPSVPAFVYGDAYVVREQIHYGGEYDASRLQKQNVCHQAAFYHRDIF
                     ALTGKYELRYSIFSDWALNLKCFGNRRIRKQYLPRVIVDFEGGGLSSTDGDADFKKDF
                     PRLVRNYLGLKSYIAYKSPRISSGIYWKIYFPFVRPFVGALRRLKNGRRES"
     gene            complement(1390..2505)
                     /locus_tag="VGB68_06915"
     CDS             complement(1390..2505)
                     /locus_tag="VGB68_06915"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY0048997.1"
                     /translation="MPYKIPVAAPSLIGNEKEYVNDCMESGWISSAGKYIELFEARFA
                     EFCGVRHAVACCNGTIALHLALVALGVGQGDEVIVPTLTFVATANAVTYCGATPVFVD
                     SEPQTWNLDPSRVEAEITPRTKGIIAVHLYGHPAKMKELKEIARRHKLFLLEDAAEAH
                     GATCDGKTVGSIGDAAAFSFYANKIITTGEGGMVVTGDDALAERLRLLRGQGMDPKRR
                     YWFPVVGYNYRMMNLPAAIGLAQLEKIDEQLKARGQIAAAYRKNLQDIDALTWQNENA
                     WARHVYWMFNVVLEPDVWAARDDVINMLSEQGIETRPVFYPMHCLPPYIDAARAGKFP
                     VAEHLARNGISLPTWTGLKSEDIDYICNALRQCKIKV"
     gene            complement(2684..>3395)
                     /locus_tag="VGB68_06920"
     CDS             complement(2684..>3395)
                     /locus_tag="VGB68_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0048998.1"
                     /translation="STITILFLGFLLGLKHAVEADHLAAVSTIVTERKSLLSSALVGG
                     VWGLGHTISLLVVGVFVLLLDFRISERTESILELFVGGMLIFLGLNVMQKIARGGTLH
                     LHTHEHGGRVHAHPHLHDEQTDAEEPHAHHGLSIGPRPLVIGLVHGLAGSAALMLLVL
                     PTIESTAIGLLYIVIFGIGSIAGMMLMSFLVSLPLQLTALHFNRANLLLRCLAGLFSI
                     GFGLWIVYEKGFADNLLG"
BASE COUNT          840 a          929 c          858 g          768 t
ORIGIN      
        1 ctaatttgag taaacgcata tgggtcaaag ctttagagtt ccaggtgata aattatattg
       61 ccggtcgcct cttcgactcc ggtctggcgc aagcccaggc ggcttgcgcc gcgcgggctg
      121 acgttgtcgg gatgaatttt cgccatcagc cgtttcgctc ccgacagccg gcaaatcagc
      181 ttggcggtat cgagcgtcaa acgcataaaa gcgccgccgc gatatttttc gtcgatcaaa
      241 actccgaagg acggaacctc gaagcccgcg tcccagccgc gcagcataaa tatgccgacc
      301 agattttcct gccagaaaac tcccgaataa acgtccgccc gatacgccga caaaatttcc
      361 gcaatcatcg cctcgtccga gccgaaagca tagaaaaatc ttgaatattc cggcggctgc
      421 gagcgcataa aatccgacac cgcggcggca tcggcgggct ggagaggacg aattaaaagg
      481 gatttcttaa acgccgtcat taaaatataa tactacgatt cacgcctgcc gtttttcagc
      541 cttcgaagcg cgccgacgaa aggtctgaca aacggaaaat atatcttcca gtaaatgccg
      601 gacgaaatgc gcggcgactt ataagcaata taagatttca aaccgagata atttctgacc
      661 agacgcggaa aatctttttt aaaatcggca tctccgtcgg tcgagcttaa gccgccgcct
      721 tcaaaatcga cgatgacgcg cggcagatac tgcttgcgga tgcgacgatt cccgaagcat
      781 ttcaggttca aagcccaatc ggaaaaaatg ctgtagcgca gctcatattt tccggtaagc
      841 gcgaaaatgt cgcggtgata gaacgcggct tgatgacaaa cgttctgctt ttgcaggcgc
      901 gacgcgtcgt attcgccgcc gtagtgaatt tgttccctga ccacataagc atcgccgtag
      961 acgaaagccg gaacggacgg cggcaaaact tcgccgaccg tctccaaaac gccttcgcgc
     1021 aatttgtcgc ccgcgccgat gaaatacaaa tattgccccg tcgccaaatc tatccccttg
     1081 ttaaaggcgt cgtaaacgcc gccgtctttt tcggaaatca gccgcaaatc gctttcatat
     1141 ttctttatat aatccagagt atcgtcggtc gatgcgccgt cgacgacaat caattcaaat
     1201 aaatctttat tctggcaaat aatcgattcg atagtccgct cgacctttcg cccgcaatta
     1261 tatgtggcga gaacgattga aaagattttt cgactgccgt ttgcttccgc cgctgacgcc
     1321 ataatgcgat gaattattaa ccgattttta ttctccggtt gaaaatttcc aaattcccga
     1381 cgcgccgcgt taaactttaa ttttacattg ccgcaacgca ttacagatat aatctatatc
     1441 ctcgcttttt aaacccgtcc acgtcggcag gctgattccg tttcgagcga gatgctcggc
     1501 gaccggaaat tttccggcgc gcgcggcgtc gatataaggc ggcaaacagt gcatcgggta
     1561 gaaaacgggt cgggtttcga ttccctgctc gctcagcata tttattacgt cgtcgcgcgc
     1621 tgcccaaacg tccggttcga gcacgacatt gaacatccag taaacgtgcc gcgcccacgc
     1681 gttctcgttc tgccacgtca gagcgtcgat gtcctgcaaa tttttccggt aggcggcggc
     1741 tatttgcccg cgggctttga gctgttcgtc tattttttcg agctgggcga gaccgattgc
     1801 ggcgggcaga ttcatcatcc ggtagttata gccgacgacc ggaaaccagt aacgccgctt
     1861 cgggtccatt ccctgaccgc gcaaaagccg caatcgttcc gccagagcgt cgtcgccggt
     1921 gacgaccatt ccgccttcgc cggtggtgat gattttattg gcgtaaaaac taaaggcggc
     1981 ggcatctccg attgagccga ccgtttttcc atcgcaggtc gccccgtgag cctccgccgc
     2041 gtcttccagc aaaaacagtt tatggcggcg ggcgatttcc ttcagttcct tcatcttcgc
     2101 cggatgaccg taaagatgaa cggcgatgat gcctttcgtg cgcggcgtaa tttcggcttc
     2161 gacgcgcgac gggtctaaat tccaggtctg cggctcggag tcgacgaaaa ccggagtcgc
     2221 gccgcagtaa gtgacggcgt tggcggtggc gacgaaagtc agtgtcggaa cgatgacctc
     2281 gtcgccctga ccgacgccga gcgcgaccag cgccagatgc agagcgattg tcccgttaca
     2341 gcaggcgacg gcgtgccgga cgccgcagaa ttcggcaaag cgagcctcga ataattctat
     2401 gtatttgccc gccgatgaaa tccatcccga ttccatacag tcattgacat actccttttc
     2461 gttgccgatt aaagaaggag cggcgacggg aattttatac ggcattttta caattggttt
     2521 cgaccgtcga gatttgattt aacatttcaa cttcggatat taagagtaac ttattattca
     2581 gcttgacgcc attttaaccg aaacggcgca gcttacgcga gagaagcgcg tgagcttaat
     2641 cgaaatcgaa actgattttg ccgccggcgg agcgtttaaa aacctatccg agcagattgt
     2701 ccgcaaaacc tttttcgtaa acaatccaca gaccgaatcc gatgctgaat aaacccgcca
     2761 gacatcttaa caagaggttg gcgcggttaa aatgaagcgc ggtaagctgc agcggcaggc
     2821 tgaccagaaa actcatcagc atcatcccgg cgatggagcc gatgccgaag ataacaatat
     2881 aaagcaagcc tatcgcggtc gattcgatgg tcggcagcac cagcagcatt aaagccgcgc
     2941 tgcccgccag tccgtgaact aatcctatga ccagaggacg aggaccaata gaaagtccgt
     3001 gatgcgcgtg cggctcctcg gcgtctgtct gctcgtcgtg caggtgagga tgcgcgtgaa
     3061 cgcgaccgcc gtgttcgtgc gtgtgcagat gaagcgttcc gccgcgcgct attttctgca
     3121 tcacattcaa tcccagaaaa atgagcattc cgccgacgaa taattccaga atactctcgg
     3181 ttcgctcgct gattcgaaaa tcgagcagca aaacaaaaac accgacgacc agcaaggaaa
     3241 tcgtgtgacc caatccccag acgccgccga ccagcgcgga gctcaagagg ctttttcttt
     3301 cggtgacgat ggtcgagacc gccgccagat ggtcggcttc gaccgcgtgt ttgagtccga
     3361 gaagaaaacc aagaaataaa attgtgatag tcgat
//
LOCUS       DASWNE010000342         2678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_11352, whole genome shotgun sequence.
ACCESSION   DASWNE010000342 DASWNE010000000
VERSION     DASWNE010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11352"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            328..792
                     /locus_tag="VGB68_06925"
     CDS             328..792
                     /locus_tag="VGB68_06925"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HEY0048999.1"
                     /translation="MKKFMFSGFMFLLCVSAAIETPAQAKPDLIVSRVGITKDATGLF
                     VQKISVTVTNGCREAEAATSYVLVTFRQNAQKDSKAIFYIGNTVKALKGGESYTQTFD
                     VGEKKIGVGRHVYVEADPYKKVSEASEDNNWRTLFPDGAGAVLNQTQCSPKR"
     gene            817..1965
                     /locus_tag="VGB68_06930"
     CDS             817..1965
                     /locus_tag="VGB68_06930"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY0049000.1"
                     /translation="MKKIILISLAVIYALVSIEAKAQPQAQQNRLQRAVSQQFLAQYA
                     DKLEAGLKGKSAGYSFTIAGGNNFMVSRAGGDARRAPDANARKMTADDKFNIASVSKT
                     ITAAAVLKLLNEKRITVDTPVHSYLPPNWTLGSNFKTVTFRELLTHRAGIRCDKEVTY
                     ENLKACVAGGINLKDKATQQYDNSNFALFRFIIPRLNGYPKTPIKVDESTLSSVYAKL
                     YADYVRQNVFAPVGLNGIDLKPAATNPALAYQFPSPVEAGDSFGDMTETSASRGWNMS
                     SKQLAVFLHNLLYTEKILPKTVSAQMKNEQLGLWKKDVAGDVVDYEHGGYYPGKDKKG
                     NLFNKGEMNTLALNFSNGVSVAIIVNSQYGPGLSIAGAAREAMKEMLK"
     gene            1980..2603
                     /locus_tag="VGB68_06935"
     CDS             1980..2603
                     /locus_tag="VGB68_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049001.1"
                     /translation="MKNNFYAINLIILAILALILSAETRAQSNDIDNPTALTSNVVEG
                     EGDGKAETVYYSFTAAKGDVKITVDAKTDDRSTPLRVALLDADGKELLPIYVVAKGAG
                     AREVGTKRFVRDTKVILKIRMDDDKDVKLLTYKIKLDGAVKFEEPPPTEQPAVSPQTP
                     TDSTTTSTESTPTIKSNADGKKVKTTDKIKKKVKAKTKETVKEIIDN"
BASE COUNT          784 a          676 c          686 g          532 t
ORIGIN      
        1 cgatacgtcc ggcgttttcc accagcacca gcagaatttc cagcgctttc ggcgttacct
       61 cggcgaccga atctccgcgc cagagaattt tttcgtcggc gtcgaggcga aattcgccga
      121 attcataaca attttccgaa atcctccgca ttcgcttttc tcctttaatt acgggctaaa
      181 tcggcgaata aagaaaaaat aaacaaaaca taaatcaaaa attacgccaa aaacaatgcg
      241 ttgtaaagga tttcggcggc aaaaaaggcg cattgtctga ttcatcaagg cacaggcgac
      301 ggaaaaaatt taaggagaag aagattgatg aaaaaattta tgttttcggg atttatgttt
      361 ttgctttgcg tttcggcggc aatcgaaacg ccggcgcagg caaaaccgga tttaattgtt
      421 tcgagagtcg gaattacaaa agacgcgacc ggtcttttcg ttcagaaaat ctcggtgacg
      481 gtaacgaacg gctgccgcga ggctgaagcg gcgacttcct acgtgctcgt cactttcagg
      541 cagaacgcgc aaaaggactc gaaggcgatt ttctacatcg gaaacaccgt gaaggcgctg
      601 aaaggcggcg aaagctacac gcagacgttc gacgtcgggg aaaagaaaat cggcgtcggc
      661 agacacgtct acgtcgaagc cgacccgtat aaaaaagttt ccgaagcgag cgaagacaac
      721 aactggcgga cgctttttcc ggacggcgcg ggcgcggtgc tgaatcagac gcaatgctcg
      781 ccgaaaaggt agaaaaataa aggaagaaaa atttctatga aaaaaataat tttaatttcg
      841 ttggcggtta tttacgctct ggtttcaatc gaggcgaaag cgcagcctca agcgcagcaa
      901 aaccggctgc agcgagccgt ctctcagcaa tttctggcgc aatacgccga taagctcgaa
      961 gccggattaa aaggcaaatc cgcgggctat tcgtttacga tagcgggcgg caataatttt
     1021 atggtcagcc gcgcgggcgg cgacgcgcgt cgagcgcccg acgcgaacgc gcgcaaaatg
     1081 acggcggacg ataaattcaa catcgccagc gtcagcaaaa ccatcacggc ggcggctgtt
     1141 ttaaaacttt tgaatgaaaa gcgaatcact gtcgatacgc ccgtgcattc ttatctgccg
     1201 ccgaactgga cgctcggcag caacttcaaa accgttacct tccgcgagct tttgacgcat
     1261 cgcgccggaa tcaggtgcga taaggaagta acttacgaaa atctgaaagc gtgcgtcgcg
     1321 ggcggcataa atttaaagga caaggcgacg cagcaatacg acaacagcaa ttttgctttg
     1381 tttcgcttta tcattccgcg tctgaacggt tatccgaaaa cgccgattaa ggtcgacgaa
     1441 agcactctat cgtccgtcta cgccaagctc tacgccgatt acgtcaggca aaacgttttc
     1501 gctccggtcg gcttaaacgg cattgacctc aaaccggcgg cgacgaatcc ggcgctggct
     1561 tatcaatttc cttcgcccgt cgaggcaggc gacagcttcg gcgatatgac cgaaacgagc
     1621 gcctcgcgcg gctggaatat gtcttccaaa cagctcgcgg tttttctgca taatctgctc
     1681 tacacggaaa agattctgcc gaaaaccgtt tccgcccaaa tgaaaaacga acagctcggc
     1741 ttatggaaaa aggatgtcgc gggcgacgtc gtcgattacg agcacggcgg ttattatccg
     1801 ggcaaagata aaaaaggaaa tctttttaac aaaggcgaga tgaacacgct cgcgctgaat
     1861 ttttccaacg gcgtgagcgt ggcgataatc gtcaactcgc aatacggtcc gggattgtcc
     1921 atcgccggcg cggcgcgcga ggcgatgaag gaaatgctga aataaaagga gaaagcaaaa
     1981 tgaaaaataa cttttacgcg attaatttaa ttatactggc gattttggct ctgattttga
     2041 gcgccgaaac gcgggcgcag tcaaacgaca tcgacaatcc gaccgctttg acctcaaacg
     2101 tcgtcgaagg cgaaggcgac ggcaaagccg aaaccgttta ttacagtttt accgccgcca
     2161 aaggcgacgt gaaaatcacg gtcgatgcga aaaccgacga ccgctcgacg ccgctcagag
     2221 tcgcgctgct ggacgccgac ggcaaagaac tgctgccgat ttacgtcgtc gccaaaggcg
     2281 cgggcgcgcg cgaggtcggc accaaacgat ttgtccgcga cacgaaagtc attctgaaaa
     2341 tccgtatgga cgacgataaa gacgtaaagc ttttgactta caaaatcaag ctcgacggcg
     2401 cggtcaaatt cgaagagccg ccgccgaccg agcagcctgc ggtttcgccg caaacgccga
     2461 ccgattcgac cacgacttca accgaaagca caccgacaat caaatcgaac gccgacggca
     2521 aaaaagtaaa aaccaccgat aaaatcaaga aaaaagtgaa agcaaagaca aaggaaaccg
     2581 tgaaggaaat tatcgacaat tagacggctt cgagtttcgc cgacagcaac gtttgtcaaa
     2641 aaaactggcg gaaccggcag gcgaaactca aaatttac
//
LOCUS       DASWNE010000343         2348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_116710, whole genome shotgun sequence.
ACCESSION   DASWNE010000343 DASWNE010000000
VERSION     DASWNE010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116710"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2335)
                     /locus_tag="VGB68_06940"
     CDS             complement(<1..2335)
                     /locus_tag="VGB68_06940"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0049002.1"
                     /translation="MAQENPLLHEALTKYIAARGRDYEQKTESKILTDAAPYLSDFIA
                     RLFGIGNQRESLLRAINEQDPIWKYKFFVQRRAAKKFTPEKINALNLTELDWALSEFK
                     FAAFGETLIHDDELSIAEITVRLLDVEEALTKNLEPTPEIDETLQKLAAAFDKLKDKT
                     FGKVFAQFANEIDETGEILQIKAVLTLLEAWSAAQFFAHQKKWYSFKVPHTLDYQKLV
                     HLTRENAGVPEAMRSLEKDLRRRDGFKLTDDRGTLRDSLYEVDYCLICHERLKDSCSK
                     GLHAPDGDIKRNPLGIKLEGCPLDEKISEMHLMKRQGDSIASLALVTIDNPMCAGTGH
                     RICNDCMKSCIFQKQEPVNIPQAETATLTDVLALPYGFEIYSLLTRWNPLNAKRPFAL
                     PYNGKNILVIGLGPAGYTLAHYLLNEGFGVVGMDGLKIEPLMDEYTGIGGRTPKPIRD
                     IAEITSELDERILSGFGGVSEYGITVRWDKNFLTMLQLLLARRERFRAYGGIRFGGTL
                     TIEDAWDFGFDHIAIATGAGRPTIVKMKNNLIRGIRKASDFLMALQLTGAFKKETLSN
                     LQVRLPALVIGGGLTGIDTATELFAYYPVQVEKMLEMFENLSAQFGEETVWNRFDEEE
                     TRVLQEFLEHGKEIRTERARAEQAGEQPDFIPLVRSWGGVSLAYRKRLQDSPAYRLNH
                     EEVQKALEEGIEFVELMNPVEAVPDEYGAVKALIFDRVNYDAESKKFENSGEMVEFPA
                     RTVCVAAGTSPNVIYEKENPGTFKLDDWKQYFAPHKLE"
BASE COUNT          508 a          636 c          607 g          597 t
ORIGIN      
        1 tctctaattt atgcggcgcg aaatattgtt tccaatcgtc gagcttgaaa gttcccggat
       61 tttccttttc gtaaatcacg ttggggctgg ttcccgccgc aacgcaaacc gtccgcgcgg
      121 gaaattcgac catttcgccg gaattttcaa attttttgct ttccgcgtca taatttacgc
      181 ggtcgaaaat cagagctttg accgctccgt attcgtcggg cacggcttcg accggattca
      241 tcagctcgac aaactcgatg ccttcttcca gagctttctg gacttcctcg tgattcagac
      301 gataggcggg cgaatcctgc aagcgcttgc gataggcgag cgaaacgccg ccccagcttc
      361 tcaccaaagg aataaaatca ggctgctcgc ctgcctgctc ggcgcgcgcg cgttctgtgc
      421 ggatttcctt tccgtgttcg agaaattcct gcagaacgcg cgtttcttct tcgtcgaagc
      481 gattccagac cgtttcttcg ccaaactgcg ccgaaagatt ttcaaacatt tccagcatct
      541 tttcgacctg caccggataa tacgcgaaaa gctcggtcgc cgtgtcgatg cccgttaaac
      601 cgccgccgat gacgagcgcc ggaagcctga cctgcagatt cgacaaagtt tcttttttga
      661 atgcgccggt caattgcaaa gccatcaaaa aatcggacgc tttgcgaatt ccgcgaatca
      721 ggttgttttt catcttgacg atggtcgggc gacccgcgcc ggtggcaatc gcgatatggt
      781 cgaaaccgaa atcccaggcg tcttcaatcg tcagggttcc gccgaaacga atgccgccgt
      841 aagcgcgaaa acgctcccgg cgcgccagca gaagctggag catcgtcaga aaatttttgt
      901 cccagcgaac ggtgatgccg tattcggaaa cgccgccgaa accggacaaa attctttcgt
      961 ccagctcgct cgtgatttcc gcgatgtcgc gaatcggttt tggcgttctg ccgccaatgc
     1021 cggtgtattc gtccattaaa ggctcgattt tcaagccgtc cattccgacc acgccgaagc
     1081 cttcgtttaa aagataatgc gccagcgtgt agcccgccgg acccagaccg atgacgagaa
     1141 tattttttcc gttgtacggt aaagcaaagg ggcgtttcgc gttgagcgga ttccatcgcg
     1201 tcagcaggct gtaaatttca aaaccgtacg gcaaagccag aacgtccgtt agagtcgcgg
     1261 tttcggcttg cgggatgttg accggctcct gcttctggaa aatgcaggat ttcatacagt
     1321 cgttgcagat gcggtgtccc gttccggcgc acatcggatt atcgattgtc accagcgcga
     1381 gcgaggcaat cgaatcgccc tgacgcttca tcaggtgcat ttccgagatt ttttcgtcga
     1441 gcgggcagcc ttccagcttg attcccagag gatttctttt tatgtcgccg tcgggcgcgt
     1501 gcaagccttt cgagcaggaa tctttcaagc gctcgtgaca aatcaggcag taatcgactt
     1561 cgtatagcga atcgcgcagc gttccgcggt cgtcggttaa tttaaaaccg tcgcggcggc
     1621 gcaaatcctt ttcgagcgag cgcatcgctt cgggaacgcc cgcgttttca cgcgtcaggt
     1681 gaacgagctt ttgataatcg agcgtgtgcg gaactttgaa gctgtaccat tttttctggt
     1741 gcgcgaaaaa ctgcgccgcc gaccaggctt cgagcaaagt caaaacggct ttgatttgca
     1801 ggatttcgcc cgtctcatcg atttcattgg cgaactgcgc gaaaactttg ccgaaagtct
     1861 tgtctttcaa tttatcgaaa gccgcggcga gtttttgcag agtctcgtcg atttccggcg
     1921 tcggctcgag atttttcgtc aaagcttctt cgacgtcgag caacctgacg gtgatttcgg
     1981 caatcgaaag ctcgtcgtcg tgaatcaaag tttcgccgaa agccgcaaat ttaaattcgc
     2041 tgagcgccca atccagttcg gtcaggttta aagcatttat tttttccggc gtaaatttct
     2101 tcgcggcgcg gcgctgaacg aaaaatttgt atttccaaat cgggtcctgc tcgttgatcg
     2161 cgcgcagcag gctttcgcgc tgattcccga tgccgaaaag acgggcgata aaatcggaaa
     2221 gatacggcgc ggcgtcggtc agaattttcg attccgtttt ttgctcgtaa tcgcgcccgc
     2281 gcgcggcgat gtatttcgtc aaagcttcgt gcaaaagcgg attttcctga gccatctcgg
     2341 cataaaaa
//
LOCUS       DASWNE010000344         6056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_11386, whole genome shotgun sequence.
ACCESSION   DASWNE010000344 DASWNE010000000
VERSION     DASWNE010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6056
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11386"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1234)
                     /gene="atpD"
                     /locus_tag="VGB68_06945"
     CDS             complement(<1..1234)
                     /gene="atpD"
                     /locus_tag="VGB68_06945"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEY0049003.1"
                     /translation="MANNGSGQLGQVVQIIGPVVDVEFSDNYLPPIYQALRITSEGFD
                     VEAKVDTICEVQQHLGEGRVRAVSMLPTDGMVRGMKAIDTGGPITVPVGDATLGRVMN
                     VIGDPVDELGAVSSTERYSIHRHAPTFDEQATELQMFETGIKVIDLIQPFLRGGKIGL
                     FGGAGVGKTVLIMELINNIAKAREGYSVFAGVGERTREGNDLLREMVESEVIKYGKDF
                     THNMEETGAFDLSKVDMNAIKDSKVALVYGQMTEPPGARLRVALSGLTVAEYFRDQGN
                     DVLFFVDNIFRFTQAGSEVSALLGRMPSAVGYQPNLATEMGEMQERITSTKTGAITSI
                     QAVYVPADDYTDPAPATTFAHLDAVTALSRQIVELGIYPAVDPLASNSTILTPDIVGD
                     EHYNTAQSVKRILQRYKDL"
     gene            1379..2173
                     /locus_tag="VGB68_06950"
     CDS             1379..2173
                     /locus_tag="VGB68_06950"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEY0049004.1"
                     /translation="MGKWMSGVLAVGAFAILYYFENRRPLRKSVEPKANNAARNLAVA
                     ALAGAAINFLEKPVARRLTKLVEKENVGLLKIFRLPKFLETALAVILLDYTLYLWHVL
                     THKAPLLWRFHKIHHADLDLTASTAIRFHFAEIAISVIFRAGQILIIGVSPKALEIWQ
                     TLLFISIFFHHSNVRLPPAFEENLELFIVTPRLHGIHHSIEESERDSNWSSGLTAWDF
                     LHKTFRRDVSQNEITIGVKEFDSLEKVSLERILFEPFAAHDKRLRG"
     gene            complement(2200..3189)
                     /locus_tag="VGB68_06955"
     CDS             complement(2200..3189)
                     /locus_tag="VGB68_06955"
                     /inference="COORDINATES: protein motif:HMM:NF005214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0049005.1"
                     /translation="MKHGQAKAEQSKTATKTNTGKAAKQNKQGKNVGKANTESPNSET
                     QPKAPMPPQKQKRPGIEAKMSPRPEFLAPNYRGAGKLLDKVALITGGDSGIGRSVAVL
                     YAREGANVAIVYLKEEEVDARETAMHIEAEGRKALLLPGDVSKASFCEKAVEKTVKEF
                     GKLDILVNNAAFQKHQKIITDISDEQFEKTFKTNIFGYFYMAKAALKHMKEGGAIVNC
                     GSITGLEGNKELLDYSATKGAIHAFTKSLAQQLVEKGIRVNCVSPGPIWTPLNVADKP
                     RKEVAKHGADTPMQRPGQPEEVAPAFVFFASDADSSYISGEILTLLGGETRAA"
     gene            complement(3256..3438)
                     /locus_tag="VGB68_06960"
     CDS             complement(3256..3438)
                     /locus_tag="VGB68_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049006.1"
                     /translation="MPRGDKDKYTDKQKRQAEHIEEGYEKQGVPEDEAESRAWATVNK
                     QDGGGNKSGSGRKKAA"
     gene            complement(3462..3671)
                     /locus_tag="VGB68_06965"
     CDS             complement(3462..3671)
                     /locus_tag="VGB68_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049007.1"
                     /translation="MIGGQTLSTKQAEENFILSQNESNPVESAEAVETIEETTFIEAK
                     TSQVDLKMKFTRLKKAMRKRSVSIF"
     gene            3848..4822
                     /locus_tag="VGB68_06970"
     CDS             3848..4822
                     /locus_tag="VGB68_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006681752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEY0049008.1"
                     /translation="MSNTTRIELPPRGLETLFGVHDQNIKFLESILDVRVNARGQDLT
                     VDGDPKDVATVQQILQDFAELFSEGNTFTDKELRDAFKQIAEDRAYSFKDYFTKARFN
                     PSGKKQVAPKTAMQRKYIDAIQNNDLTFGIGPAGTGKSYLSVAMGVQALFAKQVSRII
                     LTRPAVEAGEKLGFLPGDLQDKVDPYLRPLYDALFDLVDAERVTKMLEKRIIEVAPLA
                     FMRGRTLNDAFIILDEAQNTTGEQMKMFLTRIGFGSKVVVTGDITQIDLPRGQRSGLK
                     EAERVLADIPEIEFVYFSKKDVVRHRLVQLIVEAYEAQSGDNDRNDKG"
     gene            complement(4933..>6056)
                     /locus_tag="VGB68_06975"
     CDS             complement(4933..>6056)
                     /locus_tag="VGB68_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049009.1"
                     /translation="ELKELLAIMRRVDSQQQTTINWLNPFDQTVLETTIAYEQVAVDL
                     TAYLARTEPDEYLKEVLHFGLLEDFDHLYRYSQVLDIVEGKDPEEITQGKTDIFPGRP
                     TQDHHNDPILRLREHYSKNKAHPISKANILTVLAAEQQTMMYYREHGFQYGGQDVREL
                     YAEIAEVEEEHVTQYESLIDPNETWMEKWLMHEFVECANYYACYSMETDARIKQIWEL
                     FLSYELEHLRLAGEAIKKYEDKDPEEICGSELPTPGTFEENKEYVAEVLFDTVDKRLM
                     PNGEFARIEDLPGDWASYLYQATVNAEGSPSEQAVRYRMMSAGKELLRAGDDLIKRAG
                     EIRFALDKEMTANTAPETAEEMTSQAELLARRANASGRD"
BASE COUNT         1354 a         1627 c         1484 g         1591 t
ORIGIN      
        1 gcaaatcttt gtaacgctgc aaaatgcgct tgaccgactg cgccgtgttg taatgctcgt
       61 cgccgacaat atcaggtgtc aaaatcgtcg agttcgacgc gagcgggtca accgccggat
      121 aaattccgag ttccacaatc tgacgcgaaa gcgccgtcac ggcgtcaagg tgcgcaaacg
      181 tcgtcgctgg cgccgggtcg gtgtaatcgt ccgccggaac gtaaacggct tgaatcgaag
      241 tgatagcgcc ggtcttggtc gaagtgatgc gttcctgcat ttcgcccatt tccgtcgcca
      301 gattcggctg gtatccgacc gccgaaggca tacgtccgag cagcgccgac acttccgaac
      361 cggcttgcgt gaagcggaaa atattgtcga cgaagaacaa aacgtcattt ccctggtcgc
      421 ggaaatattc ggcgaccgtt aagcctgaaa gcgcgactcg cagacgcgct cccggcggct
      481 cggtcatctg accgtaaacg agcgctacct ttgaatcctt aatcgcgttc atatcgactt
      541 tcgacaaatc gaacgcgccg gtttcttcca tattatgggt gaaatcttta ccgtatttga
      601 taacttccga ctcgaccatt tcgcgcagaa ggtcgttgcc ttcgcgcgtt ctttcgccga
      661 cgcccgcaaa aaccgaataa ccttcgcgcg ctttggcgat gttgttaatc agctccataa
      721 tcagaaccgt tttgccgacg cccgcaccgc cgaacagacc gatttttccg cctcgcagaa
      781 agggctgaat caaatcgatg accttgatgc ccgtctcgaa catctgcaat tcggtcgcct
      841 gctcgtcaaa agtcggcgcg tgacggtgaa tcgaatagcg ttcggtcgaa ctgaccgcgc
      901 cgagctcgtc gaccgggtct ccgatgacgt tcattacgcg ccccagagtg gcatcgccga
      961 ccgggacggt aatcggaccg cccgtgtcga tcgctttcat cccgcgcacc ataccgtcgg
     1021 tcggcagcat cgaaaccgcg cgcacgcgac cttcgcccaa atgctgctgg acttcgcaaa
     1081 tcgtgtccac tttcgcttcc acgtcgaagc cttccgaagt aattctcaaa gcctgataaa
     1141 taggtggtaa ataattgtct gaaaattcta cgtcaacgac cggaccgata atctgcacca
     1201 cttgcccgag ttgaccgctt ccgttattag ccatttttaa atactcctta ctctatgaca
     1261 acaaaaaagg taggataaca aaggaataag aatatcatac cgcattcgtg taattcaaac
     1321 gagattgtga gaaaagttac aaactatcga agccggcaaa atctgttaaa atttcgctat
     1381 gggaaaatgg atgagcggcg ttttagcggt cggagctttt gcgattttgt attactttga
     1441 aaaccgccgc ccgctcagaa aatcggtcga gccgaaggcg aacaacgcgg cgcgcaatct
     1501 cgcggtcgcc gcgctcgccg gagccgcaat aaattttctg gaaaagcccg tcgcccgtcg
     1561 tttgacgaaa ctggtcgaaa aggaaaatgt cggtctgctg aaaatatttc gcctgccgaa
     1621 gtttttagaa acggctctgg cggttatttt gctcgattac acgctttatc tctggcacgt
     1681 tctgacgcac aaagctccgc ttttatggcg ttttcataaa attcatcacg ccgatttgga
     1741 tttgacggct tcgaccgcga ttcgatttca ttttgccgag atagcgattt ccgtcatttt
     1801 ccgagccgga caaattctga taatcggcgt ttcgccgaaa gcgctggaaa tctggcagac
     1861 gcttcttttc atttcaatat tttttcatca ttcaaatgtg cgcctgccgc cggcgtttga
     1921 agaaaacctc gaattattta tcgttacgcc gcgcctgcac ggcattcatc actcgattga
     1981 ggaaagcgaa agagactcaa actggtcgag cggtttaacc gcctgggatt ttctgcacaa
     2041 aacttttcgc cgcgacgttt cgcaaaacga aatcaccatc ggcgttaagg aatttgacag
     2101 tctcgaaaaa gtgagcttgg agcgaatatt atttgagcct ttcgccgcgc acgacaaaag
     2161 gctccgaggg taaggaccaa cttttataat ataaacttct caagccgcgc gcgtttcgcc
     2221 gccgagcagc gtcagaatct cgcccgaaat ataactggaa tcggcgtcgg aagcaaaaaa
     2281 cacaaaagcc ggagcgactt cttcgggctg tccgggacgc tgcatcggcg tgtccgcgcc
     2341 gtgtttggcg acttcttttc gcggtttgtc ggcgacattt aaaggcgtcc aaatcggacc
     2401 gggcgaaacg cagttgacgc gaattccctt ttcgaccagc tgctgcgcca gcgatttcgt
     2461 aaaagcgtga atcgcgcctt tcgtcgccga ataatccagc aattccttgt tgccttccaa
     2521 gccggtaatc gagccgcaat tgacaatcgc gccgccttcc ttcatatgtt tgagcgccgc
     2581 tttcgccata taaaaataac cgaaaatgtt ggttttaaag gttttctcga attgttcgtc
     2641 ggaaatatcg gtgatgattt tctgatgctt ttgaaaagcc gcgttgttta ccaaaatgtc
     2701 gagcttgccg aattccttaa cggttttttc gacggctttc tcgcagaaac tcgctttgga
     2761 aacgtcgccc ggaagcagca gagcttttct gccctcggct tcgatatgca ttgccgtttc
     2821 gcgcgcgtcg acttcttctt ccttcagata aacgatggct acgtttgcgc cttcgcgcgc
     2881 gtagagaacc gcgacggagc gcccgattcc cgaatcgccg cccgtaatca gcgcgacttt
     2941 atccaacagt ttgcccgcgc ctctgtagtt cggcgcaaga aattccgggc gcggagacat
     3001 ttttgcctcg atgcccggac gtttctgttt ttgcggcggc atcggcgctt tcggctgcgt
     3061 ttccgaattc ggcgattccg tgttcgcttt tcctacattt ttaccctgtt tgttctgttt
     3121 tgccgccttt cccgtgttcg ttttcgttgc agtcttcgac tgctcagcct tcgcctgtcc
     3181 gtgtttcata ccttcccttt gttcgtctaa aacccttgct cgctatcgaa aattcccttg
     3241 ttcgtccttt aaaggtcaag cggctttttt acgccccgaa ccgcttttgt ttcctccgcc
     3301 atcctgttta ttgacggtcg cccaagcccg gctttccgcc tcgtcttccg gtacgccctg
     3361 tttttcatag ccttcttcga tatgttcagc ctggcgtttt tgtttgtcgg tatatttatc
     3421 tttgtcgcct ctcggcatag gattttgcct ccttttaaaa tttaaaaaat acttaccgaa
     3481 cgttttctca tcgctttttt gagtcgcgta aatttcattt tcaaatcgac ctgcgacgtt
     3541 ttagcctcga taaatgtagt ttcttcgatt gtttcgaccg cttcggcgct ttcgaccgga
     3601 ttgctttcgt tttgcgacaa aataaaattt tcttccgcct gtttagtaga gagagtttgc
     3661 ccgccaatca tatcttcgaa cctcctatga tttatcttaa ggacttattg ttaccgaaag
     3721 attaattttg cgaaaagcaa catttatgcc atcgaatccg gcgccgaata cgttgacagt
     3781 ttttttgcgt tgtagtagcg tcagacagta taaaaaccat ttttacaaag aggattttga
     3841 agaaaatttg agtaacacga cgagaatcga acttcccccg cgcggtcttg agacgctatt
     3901 cggcgttcac gaccagaaca taaaatttct ggaatcaatt ctggacgtgc gcgtcaacgc
     3961 ccgcggtcag gatttaactg tcgacggcga cccgaaagac gtggcgacgg tgcagcagat
     4021 tttgcaggat tttgccgaac tgttttccga aggcaacact ttcacggaca aagagctgcg
     4081 cgacgccttc aaacaaatcg ccgaagaccg cgcttattcc tttaaagatt atttcaccaa
     4141 agcgcgtttt aacccgtcgg gcaaaaaaca ggtcgcgccg aagacggcga tgcagcgaaa
     4201 atatatcgac gcgattcaga acaacgattt aacgttcgga atcggtcccg ccggaaccgg
     4261 aaaatcgtac ctgtccgtcg caatgggcgt gcaggcgctg tttgccaagc aggtttcgcg
     4321 catcatcctg acgcgtccgg cggtcgaagc cggcgaaaag ctcggttttc tgcccggcga
     4381 tttgcaggat aaagtcgacc cttatttgcg accgctctac gacgcgctgt ttgatttggt
     4441 cgacgccgaa cgcgtcacga aaatgctgga aaaacgcatt atcgaagtcg cgccgctggc
     4501 ttttatgcgc ggaaggactt taaatgatgc gttcattata cttgacgagg cgcagaacac
     4561 gaccggcgag cagatgaaaa tgtttctgac gcgtatcggt ttcggctcga aggtcgtcgt
     4621 cacgggcgac atcacgcaga ttgaccttcc gcgcgggcag cgcagcggtc tgaaggaagc
     4681 cgaaagagtt ctggcggata ttcccgaaat cgagtttgtt tatttcagca aaaaagacgt
     4741 cgtccggcat cggctggtgc agttaatcgt cgaggcttat gaagcgcaat ccggcgacaa
     4801 cgaccgaaac gataaaggtt aaatttttcg gcgaaaagaa gaacctttaa ggctgagcgg
     4861 ataaaatcag ttcaggaaaa agcggaagaa ttaattcttc cgctttttct ttcgctcgtg
     4921 ttcgtttacg aattaatctc gtccggaggc gtttgcccga cgcgccagaa gctcggcttg
     4981 cgaagtcatt tcttcggcgg tttcgggcgc ggtgttcgcg gtcatttcct tgtccagcgc
     5041 aaaccgtatt tcgcccgcgc gttttatcaa atcatctccg gcgcgcagca gttccttgcc
     5101 cgccgacatc atccggtaac gcacggcttg ctcggacggc gagccttcgg cgttgacggt
     5161 tgcctgataa agataactcg cccaatcgcc cggcaaatct tcgattcggg caaattcgcc
     5221 gttcggcatc aggcgtttat cgaccgtgtc gaaaagcacc tcggcgacgt attccttgtt
     5281 ttcttcaaac gtgccgggcg tcggcaactc cgagccgcag atttcttccg ggtctttgtc
     5341 ttcgtatttc ttgatggctt cgcccgccag acgcaggtgt tccagctcat aacttaagaa
     5401 taattcccaa atctgcttga tgcgcgcgtc ggtttccata ctgtaacagg cgtaataatt
     5461 ggcgcactcg acaaattcgt gcatcagcca tttttccatc cacgtttcgt tcgggtctat
     5521 taacgattcg tactgcgtga cgtgttcttc ctcgacctcg gcgatttcgg cgtaaagctc
     5581 gcgcacgtcc tgaccgccgt attgaaaacc gtgctcgcga taatacatca tcgtctgctg
     5641 ctcggcggcg agaaccgtca gaatattggc tttggaaatc gggtgcgcct tgtttttcga
     5701 ataatgctcg cgcaggcgca aaatcgggtc gttatgatgg tcctgcgtcg ggcgaccggg
     5761 aaaaatatcg gtttttccct gcgtgatttc ctccgggtct ttgccctcga caatatccag
     5821 cacctggctg tagcgataca agtggtcgaa atcttccagc agtccgaaat gaagcacttc
     5881 cttcagatat tcgtccggct cggtgcgcgc cagataagcc gtcaaatcga cggcgacctg
     5941 ctcgtaggca atcgtcgttt ccagaaccgt ctggtcgaac gggttgagcc aattaatcgt
     6001 ggtctgctgc tggctgtcaa cgcggcgcat aatcgccagc agctctttta actcgt
//
LOCUS       DASWNE010000345         4234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_46130, whole genome shotgun sequence.
ACCESSION   DASWNE010000345 DASWNE010000000
VERSION     DASWNE010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46130"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="VGB68_06980"
     CDS             complement(<1..771)
                     /locus_tag="VGB68_06980"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEY0049010.1"
                     /translation="MSKNLIFIALIFIFTASVSAQQKFVANLSGLQQVPMNNSSGDAV
                     CKLTLFSNQMQWSVICNYRNLSSSLTAVRLHIGAAVGANGPIHNGVFISNGETSGVFT
                     REGSFTSQIVDDLRANRMYFVFHTTDFPGGEIRGQLHVANGAYNDFDGDGRTDLTVYR
                     TSNNTFYAHSRLTGSLIERKIGQAGDSVSLTVDFDGDGKSDFSTARYNSQVLWRIFGS
                     ASETLQETQWGNSTLGDFFAAGDYDGDGRADIAVFRAGV"
     gene            complement(866..1228)
                     /locus_tag="VGB68_06985"
     CDS             complement(866..1228)
                     /locus_tag="VGB68_06985"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEY0049011.1"
                     /translation="MNVDEQSYREKVYEIVRQIPVGKVMTYGQIAELLGEGYTARTIG
                     YVMHAADTENVPWQRVINSQGGCSTGKMTIPVNIQQKMLEDEGVSFNEKGRCDLEKYR
                     WFPEGFEPPEDEQPSLFG"
     gene            complement(1350..3197)
                     /locus_tag="VGB68_06990"
     CDS             complement(1350..3197)
                     /locus_tag="VGB68_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0049012.1"
                     /translation="MADTKILPAWAEDLRKRYLRGESSLFILHGNVYDVMLHDGKKLS
                     VTEFLTEVLLGDSRETIVVYNLSSGASFPKRAAAFSNWQEIAVEAEKAKVLAMLERSV
                     SLSDKTAVILEYAEVIAPAGDTNFQTDADRSSIVTLHRWSFLPEIERGDHIVILISEN
                     LSELSPKIVSNPKVAVIEVPMPDAETRRKIVEQTDARLSKEDAHRYAEVTAGLKAIQI
                     ASILTPPPPSEEDAASRENYISSLLGNGSDAKERAHKLATLTAGMNREEIKKLVAPEA
                     KENSEPQTNPLERAKQEADKLISKRKREILERECFGLVEFVQPEYGFEVVGGMEEVKK
                     DLLVIADSIRKGTRSRVPMGILFTGPMGTGKTFVAEAFAKECGLTTIKLKNFRSKWVG
                     ATEGNLEKILNVIKAIGQVIVIIDEGDRAFGNTDGDGDGGTSSRVIARIKEFMSDTTN
                     RGRILFLVMTNRPDKLDVDLKRAGRLDRKIPFLYSQTAEEVEMVAKALLRKNKIKTDV
                     NLTDLGEEFSAKLVGYSNADVEAVILMANDDAAREHGDSASVSKEHFTRAADDYFPSR
                     DIELLEYMELLAVFESSSRRFLPQKYAGMSPEELDLKLRELRLRVGGRR"
     gene            complement(3250..3600)
                     /locus_tag="VGB68_06995"
     CDS             complement(3250..3600)
                     /locus_tag="VGB68_06995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEY0049013.1"
                     /translation="MAKFIWQKGIEFVKEIYLITGKEGLKNDFGLKEQMRRAVASIPT
                     NIAEGFERRSREKYLNFLNIAKGSTGEIRSLLYVAFEIGYIDDAEHKHLRERAKFLSG
                     SISNHIKSIGGAEV"
     gene            complement(3657..>4234)
                     /locus_tag="VGB68_07000"
     CDS             complement(3657..>4234)
                     /locus_tag="VGB68_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049014.1"
                     /translation="ERLEYEQRKRMQQVEKLSQGGQQRVMQLIEKEKQIQQLAMQNPS
                     FTGDLLRSEITKTHNLVNSFIDLAVTCWRYENYLASVDVQQLENTRRHWQSFIESNKG
                     KADAVELEIAGKNLIIINKRIERVAEIRRYLKIAYGQINLIENSFQLLADQIVTMQSP
                     NELSGQLDELLDGVESIKETAKETEQILKTL"
BASE COUNT          958 a         1097 c         1004 g         1175 t
ORIGIN      
        1 gacgccggcg cgaaaaaccg cgatgtcggc gcgcccgtcg ccgtcataat ctccggcggc
       61 gaaaaaatcg ccgagagtgg aatttcccca ctgcgtttcc tgcaaagttt cgctcgcgct
      121 gccgaaaatt cgccagagaa cctgcgaatt gtaacgcgcc gtcgaaaaat ccgattttcc
      181 gtcgccgtca aaatctacag tcagcgaaac cgaatcgccc gcctgcccga tttttcgttc
      241 aatcaaactg ccggtcaaac ggctgtgggc gtaaaaagtg ttgttcgacg tgcgataaac
      301 cgtcaaatcc gtccgaccgt cgccgtcaaa atcgttgtaa gcgccgttgg cgacgtgtaa
      361 ttgcccgcga atctcgccgc ccggaaaatc tgtcgtgtga aagacgaaat acattctgtt
      421 tgctcgtaaa tcatcgacta tttgcgacgt aaatgagcct tcgcgagtga aaacgccgct
      481 cgtttcgccg ttcgaaataa aaactccgtt gtgaatcggt ccgttcgcac cgaccgccgc
      541 gccgatgtga agcctaacgg cagtcagaga gctgcttaaa ttccggtaat tgcagataac
      601 gctccactgc atctggtttg aaaataaagt gagtttgcag acggcgtcgc cgctcgaatt
      661 attcatcggg acttgctgca agcccgacaa attggcgaca aatttctgct gcgcgctaac
      721 tgatgctgta aaaataaaaa ttagggcgat gaagattaaa ttttttgaca taaataaatt
      781 cctgattttc aatcgtgggc ttttagaggt ttgcaccaaa tttctcaacg aaaaaattga
      841 tttgtttctt aatttataaa gctttttaac cgaaaaggct cggctgttcg tcttccggcg
      901 gctcgaagcc ttcgggaaac cagcgatatt tttccaaatc gcagcgtcct ttttcgttga
      961 aactgacgcc ttcgtcttcc agcattttct gctggatatt gacgggaatc gtcatttttc
     1021 cggtcgaaca gccgccctgc gaattgatga ctctctgcca cggcacgttt tccgtatcgg
     1081 cggcgtgcat tacataaccg atggtgcgcg ccgtgtagcc ttcgccgagc agttcggcga
     1141 tttgcccgta ggtcatcact tttccgaccg ggatttggcg gacaatttcg taaacttttt
     1201 cgcgatagct ttgttcgtca acattcataa gtttttaaag tgaaaaagca gaaaagtgaa
     1261 aatgtgaaaa agtccggcac attttgccgc actgcttttt cactttttca cactttcact
     1321 ttttcacact ttcacttttt cactttttcc tatcttcgac ctccgacgcg cagccgcagc
     1381 tcgcgcagtt ttaaatccag ttcttccggc gacattcccg cgtatttctg cggcagaaag
     1441 cgccggctcg aagattcaaa aaccgccaga agctccatat actcaagcag ttcgatgtcg
     1501 cggctcggaa aataatcgtc ggcggcgcgc gtaaaatgct cctttgagac ggaagcgctg
     1561 tcgccgtgtt cgcgagcggc atcgtcgttc gccatcaaaa ttacggcttc gacatcggca
     1621 ttgctgtaac cgaccagctt cgccgaaaat tcttcgccca aatccgtcag gttaacgtcc
     1681 gttttgattt tattttttcg caaaagagct ttcgccacca tttcgacttc ttcagccgtc
     1741 tgcgaataaa gaaacggaat ttttctatcg aggcgtccgg cgcgtttcaa atcgacgtcg
     1801 agcttatcgg ggcgattcgt catcaccaga aacaggatgc gcccgcgatt ggtcgtgtcg
     1861 gacataaact cttttatcct ggcaattacg cgcgaacttg tgccgccgtc gccgtcgccg
     1921 tccgtgttgc cgaaagcgcg gtcgccttcg tcgatgatga cgatgacctg cccgatcgct
     1981 tttatcacat tcaagatttt ctctaagttt ccttcggtcg cgccgaccca tttggagcga
     2041 aaatttttga gctttatcgt cgtcagaccg cattctttgg caaaggcttc ggcgacgaaa
     2101 gttttgcccg ttcccatcgg accggtgaac aaaatgccca tcggcacgcg cgagcgcgtt
     2161 ccctttcgga tgctgtcggc aatcaccagt aaatcttttt tgacttcttc cattccgccg
     2221 acgacttcga agccgtattc gggctggacg aattcgacca gcccgaaaca ttcgcgttcg
     2281 agaatctcgc gcttgcgttt cgaaattaat ttatcggctt cctgtttagc gcgctcgagc
     2341 ggattggtct gcggctcgga attttccttc gcctcgggcg cgaccagttt ttttatttcc
     2401 tcgcggttca ttcccgctgt cagagtggcg agcttatgcg cccgctcttt agcgtcggaa
     2461 ccgttgccga gaagcgacga aatatagttt tcgcgcgagg cggcgtcttc ttccgacggc
     2521 ggcggcggcg tcagaatcga agcgatttga atggctttca agcctgccgt cacctcggcg
     2581 taacgatgcg catcttcctt tgaaagacgc gcatcggttt gctcgactat ttttcgccgc
     2641 gtttcggcgt cgggcatcgg cacttcgata acggcgactt tcgggttgga aacgattttc
     2701 ggcgaaagct cgctcaggtt ttcggaaatc aaaatgacga tgtggtcgcc gcgctcgatt
     2761 tccggcaaaa acgaccagcg atgcaaggtt acgatggaag agcggtcggc gtcggtttga
     2821 aaattcgtgt cgccggcggg cgcgataact tccgcgtatt caagaataac ggcggtttta
     2881 tcggaaaggc tgacggagcg ttcgagcatc gccagaactt tcgctttttc ggcttcgacg
     2941 gcgatttcct gccagttcga gaaagccgcc gcgcgtttcg gaaagctcgc gcccgacgaa
     3001 agattgtaaa cgacgatggt ctcgcgcgag tctccgagca aaacttcggt caaaaattcg
     3061 gtcacggaga gctttttgcc gtcgtgaagc atcacgtcgt aaacgtttcc gtgcagaata
     3121 aataaagaag actcgccgcg caggtaacgt ttgcgtaaat cttccgccca agccggtaaa
     3181 atttttgtgt cagccataaa ttcgataagt gaaaaagcga aaaggtgaaa aagtgaaaaa
     3241 gttttgtatt taaacttccg cgccgccgat tgatttaata tgatttgaaa tcgaaccgct
     3301 caaaaacttt gctctttcgc gtaaatgttt gtgttcggca tcatcaatgt aacctatttc
     3361 aaaagcaaca tacaaaagac ttcttatttc gcctgtcgaa ccttttgcaa tattcagaaa
     3421 gtttagatat ttctcgcgcg agcggcgttc gaagccttcg gcaatattgg tcggaattga
     3481 agctacggcg cgccgcattt gttctttcaa tccaaagtcg tttttcagac cttccttccc
     3541 ggttatgaga tatatttctt tgacaaactc aattcctttt tgccaaataa attttgccaa
     3601 ataaataaat cttcaaaaca ctcgactttc tttttcactt tttcactttt tcacttttag
     3661 agcgttttca aaatttgttc ggtctctttc gcggtttctt ttatgctttc gacgccgtcg
     3721 agcaattcgt ccaattgccc ggaaagctcg ttcggcgact gcatcgtgac aatctggtcg
     3781 gcgagcagct ggaaagaatt ttcgatgaga tttatctgcc cgtaagcgat tttcagatag
     3841 cgccggattt ccgcgacgcg ctcgatgcgc ttgttgataa tgattagatt ttttccggcg
     3901 atttccagct cgaccgcgtc ggcttttccc ttgttcgatt cgataaagct ctgccagtgg
     3961 cggcgcgtgt tttccagctg ctgaacatcg accgaagcga gataattttc gtaacgccag
     4021 caggtgacgg ctaagtcgat aaaagaattg accagattgt gcgttttcgt aatttcgctg
     4081 cgcagcaaat cgcccgtaaa cgaaggattt tgcatcgcca gctgttgaat ctgtttttcc
     4141 ttttcgatta gctgcatcac gcgctgctgc ccgccctggc tcagtttttc gacctgctgc
     4201 attctctttc tctgctcgta ttccagacgc tctt
//
LOCUS       DASWNE010000346         3182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_152234, whole genome shotgun sequence.
ACCESSION   DASWNE010000346 DASWNE010000000
VERSION     DASWNE010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152234"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..734
                     /locus_tag="VGB68_07005"
     CDS             204..734
                     /locus_tag="VGB68_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049015.1"
                     /translation="MTFKLKTYSLFLVVILWGTLLGGVTYSHLVYFPVYLSALPDSSV
                     VVNGTYGLRDAVFWTRLHPVLILSLVITLALNWKLKARRGLIITTIAVYAAVLAATFL
                     YFVPELMAFQDSPNSAVSPAEWFERGQRWQIFSWIRGAAMYAAILPLLMALTNPEDGV
                     LNSQDARYLRREFSGN"
     gene            878..2284
                     /locus_tag="VGB68_07010"
     CDS             878..2284
                     /locus_tag="VGB68_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049016.1"
                     /translation="MKNLTTLLFLCAAFLIGGSIEMRAQGSVPAQSSALTEAKMPAGA
                     ERLLPESVPSEFNRAFENILKEGTGKIAGGMREVLAWDGNYKNAANVTKMVGQLQTSF
                     RAAGWQYEAGGRSGDVEVFSLYKEGLPRRVILGFFVPGDEVMVCALMEVVKPGSAAAV
                     TPKTVSTTMTSKESSATTKTDGTAVVLNVARDAHYVNVTGNEAPAMPKFPGLSPKPGK
                     VRGYVKDLSGNPLKGATIGIRSSYFAGQYSGAQGETDANGYYEFVVPKGSAHFYNAGY
                     QFEWGDGVAAVGLHPADGKLDSFTTVDGAVENFVLLPYGVTSRENVSQNPYISSSYYG
                     GSIYLSYNTVEEGDRGRIAGGIVENSIVEITLTPEGKMLDGTPGKTFIIRQPIGFKGN
                     LNIHNIPLGRYKISAKTGGKALKMKQNRKFNPLFGLMPAETTGEASLLFIPGSAKSSS
                     VTPNAGGWESVDVTLSTP"
     gene            complement(2383..>3182)
                     /locus_tag="VGB68_07015"
     CDS             complement(2383..>3182)
                     /locus_tag="VGB68_07015"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0049017.1"
                     /translation="AYRTLEPQEAYPMAKEAAMKALAIDPTLGAAYIPLASIKFAYDW
                     DFAGAENEYRQAIRLTPNNPEAHSSYATFLVAMGRTEEALNELKIARQFDPNSPNNTS
                     TIAWALYIAGRFDEAEAELKQFLKRDPNYARAYSNLSEIYMEKGKFDESLEALQKAKS
                     LAADGLTETMTAHLYAVSGRKAEALKMAANLESKARKKELPPFLLAEVYAGLNDRDKA
                     FYWLDRAVEERSNWLVFSKVSRRLKPLHDDPRFVDLLKRIGFENPSN"
BASE COUNT          727 a          775 c          879 g          801 t
ORIGIN      
        1 cgcccctttt cgcgcactaa agcttgaagc cgaaatttcg gcacgtaaac gactgacggg
       61 aagcgcaggc gatgaaaaat caaaattata tgcaattttt tttgacaaat cgccggcggg
      121 tttttatcga ccgcctcgcg cggcgcaagc ctgagaaatc cgcataaaat cgtcgttttt
      181 ctaaacctgg aggcataaat ataatgacat ttaaacttaa aacatattcg ctttttctgg
      241 tggtaatcct ttggggaacg ctgctcggcg gagtaactta ttcgcatctg gtgtacttcc
      301 ccgtttacct gtcggctttg cccgattcat ccgtagtcgt caacggaacg tacggtttgc
      361 gcgatgcggt gttttggacg cggctgcatc cggttcttat cctttcactc gtcatcacgc
      421 tcgccttaaa ttggaaattg aaagcgcggc gcgggctgat aataacgacg atagccgtct
      481 acgcggcagt gctcgcggca acctttctct acttcgttcc ggagttgatg gcgtttcagg
      541 acagcccgaa ttcggctgtc tcgcctgccg agtggtttga gcgaggacag cgctggcaaa
      601 tattcagctg gattcgaggc gcggcgatgt atgccgcaat tctgccgctg ctgatggcgt
      661 tgacgaaccc ggaggacggc gttctaaatt ctcaggacgc gcgttatctt cgccgcgaat
      721 tcagcggcaa ttaattcttt ttcaaagttt caaaattaac ttttcgccgt tgaacctttt
      781 gacacgggtt tcgcgcacta acgcctgaag ccgatatttt gagcggcatt atttatcaaa
      841 attaaaaaaa tatgacactg ggaggaataa tttgattatg aaaaatctaa cgactttatt
      901 atttttatgc gccgcgtttt tgattggcgg ttccatcgag atgcgggctc agggctcggt
      961 tccggcacag agttccgcgc tgaccgaagc aaaaatgccc gcgggagcgg agcgtttgct
     1021 gccggagagc gttccgtcgg aatttaatcg cgcttttgag aatattttga aagaagggac
     1081 gggcaaaatc gcgggcggga tgcgcgaagt tctggcgtgg gacggcaatt ataaaaatgc
     1141 ggcgaatgtg acgaaaatgg tcggtcaact tcaaacgagc tttcgcgccg cgggctggca
     1201 gtacgaagcg ggcgggcgca gcggcgatgt ggaagttttc agtttgtata aggaaggttt
     1261 gccgcgccgc gtaatactgg ggtttttcgt cccgggcgat gaggtgatgg tttgcgcgtt
     1321 gatggaagtc gtcaaacccg gctctgccgc cgccgttacg ccgaaaaccg tttcgacaac
     1381 tatgacgagt aaagaatcat ccgcgaccac aaaaacagac ggcacagcgg tggttttgaa
     1441 tgttgcccgc gatgctcatt acgtcaacgt gacgggaaac gaagcgcctg cgatgccgaa
     1501 atttcccggg ctttcgccaa aaccgggaaa agtgcgcggt tatgtgaaag acttgtcggg
     1561 aaatccttta aaaggcgcga ccatcggtat tcgctcttct tactttgccg ggcagtattc
     1621 cggcgcgcag ggcgaaacgg atgcgaacgg ttattatgaa ttcgtcgtgc cgaaggggtc
     1681 ggcgcatttt tacaacgcag gttatcaatt cgagtggggc gacggtgtgg cggcggtcgg
     1741 attgcacccg gcggacggta aattagacag ttttacgacc gtcgacggcg cggtggaaaa
     1801 cttcgtgctg ctgccttacg gcgttacgag ccgggaaaat gtcagccaaa atccgtatat
     1861 ctcgtcgagt tattacggcg gttcgattta cctctcttac aacacggtgg aggaaggcga
     1921 caggggcaga attgcgggcg gcatcgtcga aaattcgatt gtcgaaatca ccttaacgcc
     1981 ggaaggcaaa atgctcgacg gcacgccggg taaaactttt atcatccgcc agccgatcgg
     2041 ttttaagggc aatctcaaca ttcacaatat cccgctcggt cgttataaaa taagcgccaa
     2101 aacgggcggt aaagctctta aaatgaaaca aaaccggaag tttaacccgc ttttcggtct
     2161 gatgcccgcc gagacgaccg gcgaggcgag ccttttgttt atcccgggca gcgcaaagtc
     2221 ttcgagcgtt acgccaaatg ccggcggatg ggagtcggta gacgttacct tgagcacgcc
     2281 gtaggttttg aatcaattat tacattggaa aagctatggt ttttcggcgg cgggtttgtc
     2341 gattcaattc tgattttcaa ggatttttga gcaaacggtt tttcaattgc tcggattttc
     2401 aaacccgatt cgtttcaata aatcaacgaa tcgcgggtcg tcgtgaagcg gttttaatcg
     2461 gcgcgagact ttgctgaaga ccagccaatt cgagcgctcc tcgacggcgc ggtcaagcca
     2521 gtagaacgct ttgtcccggt cgttcaaacc ggcatagact tccgccagta aaaacggcgg
     2581 aagctccttt ttccgggctt tcgattccag attcgccgcc attttcaacg cttccgcttt
     2641 gcgccccgaa accgcataaa gatgggcggt catcgtttcc gtcagcccgt cggcagctag
     2701 acttttggct ttttgcagcg cttcgagcga ttcgtcgaat ttaccctttt ccatataaat
     2761 ttcgctgaga ttcgagtagg cgcgggcgta attcgggtcg cgcttgagaa attgtttgag
     2821 ctccgcctcc gcttcgtcga aacgcccggc aatgtataaa gcccaggcga tagtcgatgt
     2881 attattcggc gaattggggt cgaactgtcg agcgattttc aattcattta aagcttcttc
     2941 ggtgcgtccc atggcaacca gaaaagtcgc gtaggaagaa tgagcttccg gattgttcgg
     3001 cgtcagcctg atggcttgcc ggtattcgtt ttcggcgccc gcaaaatccc agtcgtaagc
     3061 gaattttatc gaagccagag gaatgtaagc cgcgccgagc gtcgggtcga ttgccagagc
     3121 tttcatcgcc gcctctttcg ccatcggata tgcttcctgc ggctcgagcg tgcgataggc
     3181 gg
//
LOCUS       DASWNE010000347         2470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_11537, whole genome shotgun sequence.
ACCESSION   DASWNE010000347 DASWNE010000000
VERSION     DASWNE010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11537"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..1171)
                     /locus_tag="VGB68_07020"
     CDS             complement(167..1171)
                     /locus_tag="VGB68_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049018.1"
                     /translation="MNPQKQKNRFIAFNSLGERQTDIDTPLGDAAFDSRHNCTYTIED
                     VVQTETVLDCQQQDIHEETVESQMKKLTVTYTSVTAQIIAFWLAFFLSAAAAPVTVGS
                     KKKHALTRSTDDDLAAFGFVEGFEDDSTPAQKYKGFKVDSLAFTLNRRKNVGLTVVAY
                     GHFETEDVTEDFVLPACVNLPALKGRDCKFLLNAVNKSSLLWQNNFTVNNSIPTGDDA
                     FPFDDINVENLERGAQPTYPGNVQILGSKGDTPYTDAEARARQAVKVQFGADAADRVE
                     LSFPSTQLKLANPPTVFVGELNRSAINIDLNPHKDATLGAPLKADAFLSQTEAFLLTA
                     "
     gene            complement(1317..1520)
                     /locus_tag="VGB68_07025"
     CDS             complement(1317..1520)
                     /locus_tag="VGB68_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049019.1"
                     /translation="MKKLRIKFVGKDHEAPKFLIDGNLRIDLPENQSKPFPHDHAKRI
                     LKVASEMYKPVVRLGRKRKASAL"
     gene            complement(1857..>2470)
                     /locus_tag="VGB68_07030"
     CDS             complement(1857..>2470)
                     /locus_tag="VGB68_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049020.1"
                     /translation="DVKQPLGRFQHTLAEKTDVLKMLKSINAKLVEVGGDSLKDNLLE
                     EIFEESWAKLEVRLNKAKEAIKTQSNDEDTAPAENLRKESDKLDEILEILRTQQRLSI
                     SEEIDHFVPKGKTQIKAQTYSGILVHLSLNEKLSKVDEKYITDYLKQYLPVETVAQLA
                     LTFTSLAYRIIFGKPISLSKIKYGLEQFQIKTELVVEGFDLFE"
BASE COUNT          629 a          530 c          584 g          727 t
ORIGIN      
        1 aatattcctg ttcggctttt tcatcttctt ttacaccgag aagtttttgt gttagctgtt
       61 cttcttcagc tatccctatc atttgcagaa aaaagctgta agttttgtcg taagtcgcat
      121 taacttgaat tatttctgag aacatttaaa aaatctcctg tttaatttat gcggtaagca
      181 aaaaagcttc cgtttgtgag aggaatgcat ccgctttgag cggcgcgccc aaggtggcgt
      241 ctttgtgcgg attgaggtcg atgttaatcg ccgagcggtt cagctcgccg acgaaaaccg
      301 tgggcggatt ggcgagcttg agctgcgtgc tcggaaaact taattccacg cgatcggcgg
      361 cgtccgcgcc gaactgcacc ttgacggctt gcctggcgcg agcttcggcg tcggtgtacg
      421 gcgtatcacc cttcgagcct aagatttgca cgttgcccgg ataagtcggc tgcgcgccgc
      481 gttcgagatt ttcgacgttg atatcgtcga acgggaaagc gtcgtcgccg gtcggaatcg
      541 aattgttgac cgtaaaattg ttctgccaca aaagcgacga tttattgaca gcattcaaaa
      601 ggaatttgca atctctgcct ttgagtgcgg gcagattgac gcaagccggt aaaacaaaat
      661 cttcggtgac gtcttccgtt tcgaaatgac cgtaggcaac aactgttaag ccgacgtttt
      721 tccggcgatt taacgtaaac gccagagaat cgactttgaa gcctttatac ttttgcgccg
      781 gcgtcgaatc gtcctcgaag ccttcgacga aaccgaaagc ggctaaatcg tcgtcggtcg
      841 agcgcgtcag cgcgtgtttc tttttcgagc cgaccgtgac gggagccgcg gcggcactca
      901 ggaagaaagc gagccagaac gcgatgattt gcgccgtgac gcttgtgtaa gtgaccgtca
      961 gcttcttcat ctgcgattcg accgtttctt cgtgaatgtc ctgctgctgg caatctaaaa
     1021 cggtttcggt ctgcacgacg tcttcaatcg tgtacgtgca gttatggcgg gaatcaaaag
     1081 cggcgtcgcc gagcggcgta tcgatgtccg tctggcgctc gccgagtgag ttgaaagcga
     1141 taaagcggtt cttttgtttt tgagggttca taagaggtaa aaggtaagag gtgaggacta
     1201 aaaggtaatc ggcgcgcctc gagaagcatc attaacggaa aagataacta ccgccgtata
     1261 tgtgacgagg aatgagtaac cccgttccgt ttctcgcgac ggcggcgcgc cgaattttaa
     1321 agagctgaag ctttgcgctt tctgccgagt cgcacaaccg gcttatacat ctccgaagcg
     1381 acttttaaaa tgcgtttcgc gtggtcgtga gggaaaggct tggactgatt ttcgggtaaa
     1441 tcaatgcgga gattgccgtc gattaaaaat ttcggcgctt cgtggtcttt gccgacgaat
     1501 ttgattctga gttttttcat ttctttttaa atctgtttcg ccagttgatt tccacaaacg
     1561 agtcgccatc ttcgtaagtg ccgtaatcta gttcaatttt aaaaacagtt accagcagca
     1621 gcattaaaag taagccgagt acaaatccaa tcattaagaa atctccactt tgcagactaa
     1681 atcgacgtaa aagccgtaga cgcccgtcag cgggcatcac tgaataaatg tatcaaaatt
     1741 aaatctagcg gtggtgtgcg cggattttcg gcaataaaaa agaggctcgt ttgagcctcg
     1801 cgggtgtggg ttgaaatgat tgacggttag atgattttaa atatataaac aaagacttac
     1861 tcaaacaggt caaatccttc tactacaagc tctgttttaa tttgaaattg ttcaagacca
     1921 tattttattt tgcttaatga aataggcttg ccaaaaatta tacggtaagc gagactagta
     1981 aaagtaagag ctaattgcgc caccgtttca acaggcaagt attgtttaag ataatcagta
     2041 atatattttt catcaacctt agatagtttt tcattcaaag ataaatgaac caatattccg
     2101 ctataagttt gagctttaat ttgtgttttg ccttttggaa caaagtggtc tatttcctca
     2161 gatattgaaa gtctttgttg agttcttaaa atttctaaaa tctcatcaag tttatcgctt
     2221 tccttgcgaa gattttcagc aggcgcggtg tcttcatcat ttgattgagt tttaattgct
     2281 tcttttgctt tattaagacg gacttccagt tttgcccacg attcttcaaa aatttcttcc
     2341 agtaaattat ctttaagcga atcacctccg acttcgacaa gtttcgcgtt aatggatttg
     2401 agcattttca aaacgtccgt tttttcggct aaagtatgct gaaatcttcc caaaggctgt
     2461 tttacgtccg
//
LOCUS       DASWNE010000348         4401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_541252, whole genome shotgun sequence.
ACCESSION   DASWNE010000348 DASWNE010000000
VERSION     DASWNE010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541252"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VGB68_07035"
     CDS             <1..972
                     /locus_tag="VGB68_07035"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY0049021.1"
                     /translation="AALLAVFLRGLAELTRRYTKLSEGLSVLLVAALLVGILALAISM
                     LAPSVAEEVRHLRDELPKSAQKAGEYISRFGWGRTIIEQLPSADEVIEKIDASSLLTR
                     VGGYFSSTVGAIANFFIVILLAIYLATEPQTYIRGFSKLFTFDKRLRVVEVFNTIGET
                     LQMWLVGKVASMLFIGLLTWVGLWIIGVPLSFTLGLIAGLLSFIPNFGPILSAVPAIL
                     LAFIASPVDALYVTGLYVGVQLIESNVVTPIIERETVELPPALTIVSQLALSVLIGGL
                     GLVLATPILAVIMVLVQTVYIEDILGDKITEVREKDLEEEKDAKARA"
     gene            1151..3460
                     /locus_tag="VGB68_07040"
     CDS             1151..3460
                     /locus_tag="VGB68_07040"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY0049022.1"
                     /translation="MFNRQHRNRNKFVLIWILLFSIGLAAQTPQVDTSLLSLDRIFNS
                     NDFGGKGIGGVRWLKSGDAYSKIEPSTTVKGGSDLVSYDAATNKRTVLIPAEKLIPAG
                     AAAPLPLNGYDWSADNRQMLIYTNSKKVWRLNTRGDYWVLDIAGGSLKKLGGDAKPST
                     LMFAKFSPDGTRVGYVRENNIYVENLADGRITQVTTDGSKNLINGTSDWVNEEEFYLR
                     DCWRWSPDGKSIAYWQFNTEGSKDFVLVDNTKDLYPVLNYFPYPKAGTTNAAVRAGVV
                     SAGGGETKWINTPGDLRQNYIVMVEWMDNSNELILQHMNRLQNTDQVMIADAKTGAVR
                     TILTEKDDAWIEVMMPRLRWVDGGKRFLWMSERDGWQHLYTVSRDGSDVKLITPGNFD
                     VISLSGYDEANGWLYYLASPDNATQRYLFRSKIDGSGAAERVTPEAQKGWHNYNISPK
                     GTWAIHNYSAFTSAPRFEVVNLTNKTVARTMVDNAELQAKIDKLKKGPQEFFRIDIGD
                     GVALDGYMLKPPGFDPSKKYPILFYAYGEPAAQTVIDAWGDTEYLWHLMLSQQGYIVA
                     SVDNRGTPAPRGRAWRKAIYRKNGIVNSADQAGAVKAMIAKMPFIDASRVGIWGWSGG
                     GVSTLNAMFRYPDIYKMGMAVAPVPDQRFYDTIYTERYMGMPQENAADYKQSSPITHA
                     AGLKGDLLIVHGTGDDNVHYQGTEVLIDELIKLNKPFSMMAYPNRTHSISEGENTTRH
                     LYGLLTRYLNEHLPVSAEQQKEMQKSASR"
     gene            3849..>4401
                     /locus_tag="VGB68_07045"
     CDS             3849..>4401
                     /locus_tag="VGB68_07045"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEY0049023.1"
                     /translation="MKKLFFGLLAVVMMVGFQVTDASAQMSGGNPMVGGAAMYKTKDI
                     VDNAVNSSDHTTLVAAVKAADLVATLKGKGPFTVFAPTNAAFDMLPAGTVSTLLKPEN
                     KSMLAGILTYHVVAGNMDSKAIMKAIKKGKGEATLTTVAGGRLMAKMNGKTLVLTDEK
                     GRMSTVTIADVRQSNGVIHVVDSV"
BASE COUNT         1070 a         1078 c         1231 g         1022 t
ORIGIN      
        1 gcggcgctgc tggcggtttt cctgcgcggt ctggcggagt tgacgcggcg ctacacaaag
       61 ctttccgaag gcttatcggt tctgctggtc gccgccctgc tggtcggcat tctggctctg
      121 gcgatttcga tgctcgcgcc gagcgtcgcc gaagaagtca ggcatttgcg cgacgagctg
      181 ccgaaatcgg cgcagaaagc cggtgaatat atttccagat tcggctgggg cagaacgatt
      241 atcgagcagc tgccgagcgc cgacgaggtg atagaaaaga ttgacgcgtc gagcctcttg
      301 acgcgcgtcg gcggttattt ttcttcgacg gtcggcgcga ttgccaattt tttcatcgtt
      361 attttattag cgatttatct ggcgaccgag ccgcagactt atatcagagg cttctcaaaa
      421 ttatttactt tcgataaacg gctgcgcgtc gtcgaagtct ttaacaccat cggcgaaacg
      481 ctgcaaatgt ggcttgtggg gaaagtcgct tcgatgctgt ttatcggtct gctgacgtgg
      541 gtcggattgt ggattatcgg cgttccgctt tcttttacgc tcgggcttat tgccggactt
      601 ttatcgttta ttccgaattt cggaccgatt ctttcggcgg ttccggcgat tctgctggca
      661 tttatcgcca gcccggtcga tgctttatac gttaccggtt tgtatgtcgg cgtgcagctt
      721 atcgaatcaa acgtcgtgac gccgataatc gagcgcgaaa cggtcgagct gccgcccgcg
      781 ctgacgattg tctcgcaatt ggctctgagc gtgcttatcg gcggcttggg tttggtgctg
      841 gcgacgccga ttctggcggt gataatggtc ctggtgcaga cggtttatat agaagatatt
      901 ctgggcgata agattacgga agtgagggaa aaggatttgg aagaggaaaa agacgcgaaa
      961 gcgcgcgcat aagccggaaa agcgtcgaat cggaaagcct tcatttaaat tttgcccgcc
     1021 cgctttgcgc cgaacttgag gagagttaca atagccgcaa ttcgaaaacc gcaattcgca
     1081 gttttacacg ctatttcgcc ggtagaatcg tctgccgtaa aagtaaaaat caaatatttc
     1141 gggagtaatt atgtttaatc gtcaacatcg caatcgcaat aaattcgttt taatctggat
     1201 tctattgttc agtatcggtt tagccgccca gactccgcag gtcgatactt cgctgttaag
     1261 tctcgaccgt atatttaact ctaatgattt cggcgggaaa ggcatcggcg gggtcaggtg
     1321 gctgaaatcc ggcgacgctt attcgaaaat cgagccttcg accaccgtca aaggcgggtc
     1381 ggatttggtc agttacgacg cggcgaccaa caagcgcacg gttttaatac cggcggaaaa
     1441 actgattccg gcgggagccg ccgcgccgct gccgctcaac ggctacgact ggtcggctga
     1501 caaccggcaa atgctgattt acaccaactc gaaaaaggtc tggcggctca acacgcgcgg
     1561 cgactactgg gttttggaca ttgccggcgg aagcttgaaa aagctcggcg gcgacgccaa
     1621 gccatcgacc ctgatgttcg ccaaattctc gcccgacggg acgcgcgtcg gatacgtcag
     1681 ggaaaataat atctacgtcg agaatctggc ggacgggcgc attacgcagg tgacgaccga
     1741 cggctcgaag aatctgatta acggaacttc ggattgggtc aacgaggaag aattttacct
     1801 gcgcgactgc tggcgctgga gtccggacgg caaatcaatc gcttactggc agtttaacac
     1861 ggaaggctca aaggatttcg tgctggtcga caacacgaaa gacctttatc cggttttgaa
     1921 ttattttcct tacccgaaag cgggaacgac gaacgcggcg gttcgcgccg gagtcgtcag
     1981 cgcgggcggc ggcgagacga aatggataaa cacgccgggc gatttgcggc agaattatat
     2041 cgtgatggtc gaatggatgg acaattcaaa cgaattgatt ctccagcata tgaaccgttt
     2101 gcagaacaca gaccaggtga tgatcgccga cgcgaaaacc ggcgcggtgc ggacgattct
     2161 gaccgaaaaa gacgacgcct ggattgaagt tatgatgccg cggctgcgct gggtcgacgg
     2221 cggaaaacgt tttctatgga tgagcgagcg cgacggctgg cagcatcttt acacggtttc
     2281 gcgcgacggc tcggacgtaa aattaatcac gccgggcaat ttcgacgtga taagtctctc
     2341 gggttatgac gaggcgaacg gctggcttta ttacctcgct tcgcccgata acgcgacgca
     2401 gcgttatctt ttccgctcaa aaatcgacgg cagcggcgcg gctgagcgcg tcacgcccga
     2461 agcgcagaag ggctggcata actacaacat ctcgccgaaa ggcacctggg cgattcacaa
     2521 ttattcggct ttcacgagcg ctccgcgctt tgaggtcgtt aatttaacga ataaaacagt
     2581 cgcccgcacg atggtcgata acgccgaatt acaggcgaaa atagacaagc tcaaaaaagg
     2641 accgcaggaa tttttccgca tcgacatcgg cgacggcgtc gcgctcgacg gttatatgct
     2701 gaagccgccc ggatttgacc cgtccaaaaa atatccgatt ttgttttacg cttacggcga
     2761 gccggcggcg caaaccgtca tcgatgcgtg gggcgacacc gaatatttgt ggcatttaat
     2821 gctttcgcag caaggttata tcgtcgccag cgtcgacaat cggggaacgc ccgcgcccag
     2881 aggacgcgct tggcgcaaag ccatctatcg aaaaaacggc atagtcaatt ccgctgatca
     2941 ggcaggcgcg gtgaaagcga tgattgcgaa aatgcctttt atcgacgctt cgcgcgtcgg
     3001 tatctggggt tggagcggcg gcggcgtatc gactctgaac gcgatgtttc gctacccgga
     3061 catttataag atgggaatgg cggtcgcgcc ggttcccgac cagcgttttt acgacacgat
     3121 ttacaccgag cgctatatgg gaatgccgca ggaaaacgcc gccgattaca agcagagctc
     3181 gccgattact catgcagccg gcttgaaagg cgatttgctg atcgttcacg gcacgggcga
     3241 cgataacgtt cattatcagg gcacggaagt tttaatcgac gagttaatca aactcaataa
     3301 gcctttctcg atgatggctt atccgaacag gacgcacagc atttcggaag gagaaaatac
     3361 gacgaggcat ctgtatggtt tattaactcg ttatttgaat gaacatttgc cggtttccgc
     3421 cgaacagcaa aaggagatgc agaaatccgc ttcgcgataa gcgggagtcc gcgacagcag
     3481 cggcggcttt gaaattgaag ccgagttttt taatttatgc cggcaggata atccgcattc
     3541 agccataacg gcacggaaat cttttggcag ctaacaaaag gctggaacct ttaccgattt
     3601 tccagccttt tgtcctaagc aaacgtgggc tattaataaa aaatccttaa tcgccgccgc
     3661 tttccaatta cctcgagccc gcgcctgatt cggaaagccg ataatcggta atcttccggt
     3721 tacataaaaa taataaataa gtctcccgcc cgatttgtct gcatccgttt ttccggcgac
     3781 ggcgaaatac ctatcaaagg aaacatttgt ttcttttaat ttaagtaaag acaagtaagg
     3841 gagattttat gaagaaatta ttttttggct tattggctgt cgttatgatg gtcggatttc
     3901 aggtaacgga cgcatccgcg cagatgtcgg gcggcaatcc gatggtcggc ggcgcggcaa
     3961 tgtacaagac gaaggacatt gtagacaacg cggttaattc ctcagaccac acgacgctgg
     4021 tcgctgccgt taaagcagcg gatttggtcg cgacgctgaa aggcaaagga ccgtttacgg
     4081 ttttcgcgcc gaccaacgcg gcgtttgata tgctgcccgc cggaacggtc agcacgcttt
     4141 tgaaaccgga aaacaaatcg atgctggcgg gaatcctgac ttatcacgtc gtcgccggca
     4201 atatggattc caaagcaatt atgaaagcca tcaaaaaagg taagggcgaa gcgactttaa
     4261 cgactgtcgc gggcggcagg ctgatggcta agatgaacgg caaaactctc gttttgacgg
     4321 acgaaaaagg cagaatgtcg acggtcacga ttgccgacgt gagacaatcg aacggcgtta
     4381 ttcacgtagt cgactcggtt t
//
LOCUS       DASWNE010000349         3351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_363386, whole genome shotgun sequence.
ACCESSION   DASWNE010000349 DASWNE010000000
VERSION     DASWNE010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363386"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VGB68_07050"
     CDS             complement(<1..410)
                     /locus_tag="VGB68_07050"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY0049024.1"
                     /translation="MRKTAPKVLSKHLTALVVVWTLLILPVAGFGQTRISMPRNKYKV
                     QDDIKLGRDASRQVEQQMPILNDAEATRYVQQVGQRLVNAIPPEFQQTQFEYTFQIVN
                     ARDINAFALPGGPMYVNRGMIEAAQSEGEMAGVMA"
     gene            677..1297
                     /locus_tag="VGB68_07055"
     CDS             677..1297
                     /locus_tag="VGB68_07055"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEY0049025.1"
                     /translation="MAFSLLPREEEYFAFFKEMTAQIQEGADALVAMMQDTSESFESH
                     TKRIKNAEHACDELTHKITTKLNKSFITPFDREDIYMLVVALDDVLDYIDAGARAILM
                     YDIREINDHAKQFAKVIQSLAMELQSAISILAKPNGMNQHIVEIHRLENEADDVYFRA
                     IGELFRNTTDPITIIKWKEVYEILEAATDRCESVANIIESVILKHN"
     gene            1386..2429
                     /locus_tag="VGB68_07060"
     CDS             1386..2429
                     /locus_tag="VGB68_07060"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEY0049026.1"
                     /translation="MEATTALVVFIIVVALIFDYTNGMHDAANSIATVVSTRVLSPGA
                     AVAWAAFFNFIAFAVFGTAVAKMIGGGLVDLKPIPSELHLYVLLSGLVGVIAWNIITW
                     YLGLPTSSSHALIGGYAGAAIAANGGFSVIIPSGWYLTLTFIIGAPLMGMTLGFVLML
                     SVYWIFRNTSVARVDKGFRVGQLFSAAFYSLGHGGNDAQKTMGIITAVLVAGGMLQAE
                     AGGKLPEIPLWVVLSAHAAIGLGTLTGGWRIVKTMGSKITKLQPVGGFCAETAGAITL
                     AFATHFGYPVSTTHTITGAIVGVGSTKRLSAVKWGVAGNLVWAWILTIPMAGLIAAVC
                     YFIILLIHRTTGF"
     gene            complement(2494..2757)
                     /locus_tag="VGB68_07065"
     CDS             complement(2494..2757)
                     /locus_tag="VGB68_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049027.1"
                     /translation="MSSFAWIIIIVLALTLLGTLTLGFITVRYYWGERGTPPLTGEER
                     RRQREEELRLRTKQLEYAKQNPIATRKSDFWDNSKVYKDKNTG"
     gene            complement(2875..>3351)
                     /locus_tag="VGB68_07070"
     CDS             complement(2875..>3351)
                     /locus_tag="VGB68_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY0049028.1"
                     /translation="GEIEVFCSNCGSRINTELNFCSRCGTKVVKNDSETQKSVSENLS
                     SSLGYIGGFGLIGFIFVALVLVRNNVPVRALMAISLFYLGALFGICYLILQQIAVASS
                     AGKSSAPVSDFRNNFQPEQLNPANTAQLESPREEPAGVSVTENTTKTLDEVLLKRN"
BASE COUNT          787 a          842 c          860 g          862 t
ORIGIN      
        1 gccatgacgc ccgccatttc gccttcggat tgagcggctt cgatcattcc gcgattgacg
       61 tacatcggtc cgcccggcag cgcgaaagcg ttgatatcgc gggcgttgac gatttggaac
      121 gtgtattcaa actgcgtttg ctgaaattcc ggcggaatcg cgttgactaa tctttgaccg
      181 acctgctgca cgtagcgggt cgcttccgca tcgttcaaaa tcggcatttg ctgctcgacc
      241 tggcgcgaag cgtcgcgtcc gagcttgatg tcgtcctgga ccttgtattt gttgcgcggc
      301 atcgagatgc gcgtttgacc gaatcccgcc accggcagaa tcaataatgt ccaaactacc
      361 accaacgccg tcaaatgttt gctcaaaact ttcggagctg ttttcctcat tttctcctcc
      421 aaatcccgaa gtcttatttc ctgtaactgt taatcttacc gaagaatccg ctttataatc
      481 aataaaaaga acaattcgta tttctgagat gaaagccgcc ggacaaagtt tgccgctcta
      541 tttcgagtaa aaaatgactg atttcgtgtg acgttacatt tatgttaaat aaatttttct
      601 tttaatagac aaggcagatt actttggcta tattcaatta attccttttt aaattaaagt
      661 tggagaatat cgaaaaatgg cttttagttt gcttccccgc gaagaagagt attttgcttt
      721 ctttaaggaa atgacagcgc aaattcagga aggcgcggac gcgctcgtgg cgatgatgca
      781 ggatacgagc gagagctttg aatctcacac gaaaagaatc aagaacgccg agcacgcctg
      841 cgacgagtta acgcataaaa tcacgaccaa gctgaacaaa tcgtttatca cgccgtttga
      901 ccgcgaagat atttatatgc tggtcgtcgc gctggacgac gtgctcgatt acatcgacgc
      961 gggcgcgcgg gcgattctga tgtatgacat ccgcgaaatc aacgaccacg ccaagcagtt
     1021 cgccaaggtc attcagagcc tggcgatgga gctgcaatcg gctatctcga ttctcgccaa
     1081 accaaacggg atgaaccagc acatcgtcga gatacatcgc ctcgaaaacg aagccgacga
     1141 cgtttatttc cgtgccatcg gcgagctttt ccgcaacacg accgacccga taaccataat
     1201 taaatggaag gaagtctacg aaattctgga agccgctacc gaccgctgcg aatcggtcgc
     1261 caatattatc gaaagcgtta tcttgaaaca caactaatcg gcggtaagcg gtagacggta
     1321 agcggtaagt tgagaaaact aacttaccgc ttaccgttta ccacttaccg tttaccactt
     1381 attttatgga agctacaact gcgctggttg tttttatcat tgtcgtcgcg cttattttcg
     1441 actacacgaa cgggatgcac gacgccgcca actcgattgc gacggtcgtc tcgacgcgcg
     1501 ttttgtcgcc gggcgcggcg gtcgcgtggg cggcattttt taactttatc gcctttgccg
     1561 ttttcggaac ggcggtggcg aaaatgattg gcggcggtct ggtagattta aagccgatac
     1621 cgagcgagct gcatctttac gttctgttgt ccggattggt cggcgtcatc gcctggaata
     1681 taattacctg gtatctgggt ttgccgacat cgagctcgca cgcgctcatc ggcggctacg
     1741 cgggcgcggc aatcgcggca aacggcggct tcagcgtaat cattccatcg ggctggtatc
     1801 tgacgctgac ctttatcatc ggcgcgccgc tgatgggaat gacgctcgga tttgtcctga
     1861 tgctctcggt ttactggatt ttcagaaata cgtcggttgc cagagtcgat aaaggctttc
     1921 gcgtcgggca gcttttctca gccgcttttt attcgctcgg gcacggcgga aacgacgcgc
     1981 agaaaacgat gggaatcatc acggcggtgc tggttgccgg cggtatgctg caagccgagg
     2041 cgggcggcaa gcttccggaa attccgctct gggtcgtgct ttcggcacac gcggcaatcg
     2101 ggctcggaac cctgacgggc ggctggcgca tcgttaagac gatgggctca aaaatcacca
     2161 agctccagcc ggtcggcgga ttctgcgcgg aaacggcggg cgcgattacg ctggctttcg
     2221 ccactcattt cggctatccg gtttcgacga cgcacacgat taccggcgca atcgtcggcg
     2281 tcggctcgac caagcgcctt tcggcggtta aatggggcgt cgccggcaac ctcgtctggg
     2341 cgtggatttt aacgattccg atggcgggat tgattgcggc ggtctgttat tttattattc
     2401 tgctgattca ccggacgacc ggattttaga tgctgaaaat aaaactgtaa atatcataga
     2461 cgagcgcccg tgagaaatcc cggggcgacc atcttagccg gtgtttttat ctttgtagac
     2521 cttgctattg tcccagaaat cgctcttgcg cgtggcaatc ggattttgtt tggcgtattc
     2581 gagttgtttg gtccggaggc gtaattcttc ttctctctgc cgacgtcgtt cttctccggt
     2641 aagcggcggc gttcctcttt cgccccaata atatcttacg gtgataaaac ccagagtcag
     2701 agttcccagc agagtcagag ctaaaactat gataataatc caggcaaaac tagacatacg
     2761 ccgattttaa cactctgttc agaggtttac aaaattttcg attgtcacga aaaggctcgt
     2821 ttatgagcca aacggagcgc tgccggaaag ccggattttt ttcaactttt ccctttaatt
     2881 tcgttttaat aaaacttcgt cgagggtttt ggtcgtattt tcggtgacgc tgacgccagc
     2941 cggttcctcg cgcggagatt cgagctgcgc cgtgtttgcc ggattgagct gttcaggctg
     3001 aaagttattg cgaaaatcgg aaaccggcgc ggatgatttt ccggcagaag aagcgacggc
     3061 gatttgctga agaattaaat agcagatgcc gaaaagcgcg cccagataaa aaagcgagat
     3121 tgccatcaga gcccttacgg gaacgttgtt tcttaccagc acgagagcca caaaaataaa
     3181 tccgatgagc ccgaaaccgc cgatatatcc gagcgacgac gaaagatttt ccgagacgga
     3241 tttctgcgtt tccgaatcgt ttttgacaac tttcgttccg catcgactac agaaattcag
     3301 ttcagtattt atccggctgc cgcaatttga acaaaacacc tcgatttctc c
//
LOCUS       DASWNE010000350         3158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_152286, whole genome shotgun sequence.
ACCESSION   DASWNE010000350 DASWNE010000000
VERSION     DASWNE010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152286"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            289..468
                     /locus_tag="VGB68_07075"
     CDS             289..468
                     /locus_tag="VGB68_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049029.1"
                     /translation="MEQERISKYERGVPEPPLYVLVAYADVANVYVEVLIKDDLDLPE
                     SLPSRKKSEGIKRQK"
     gene            537..1586
                     /gene="tilS"
                     /locus_tag="VGB68_07080"
     CDS             537..1586
                     /gene="tilS"
                     /locus_tag="VGB68_07080"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEY0049030.1"
                     /translation="MNNFVRNLLTEWRRLNLPCEDETFVVAVSGGADSLSLALALDDL
                     KKRKKLNLRFVVAHFNHNLRGAESEADEKFVKDLAAEYDFELALGRGDISEKGNLEQS
                     ARVARYDFLTGVAENLRARGVLTAHTINDQAETFLLNLIRGSGIEGLSAMKPLRLLER
                     ETRRRGDAQTETQDLSSNSALSTQHSALILVRPLLGWAKRSDTENYCHAEKIEYRYDS
                     MNEDLAFRRVRIRRVLLPLLQDFNPKIIARLAATAALLRDEHDELEKLANHQLNQANK
                     DELPTRDLTNLSPAIRRRVLRAWLASRRGDLRRLDLKHLEAVERLIFSRKSGRFVELP
                     GGETVVKTNGKLVFK"
     gene            1753..>3158
                     /gene="ftsH"
                     /locus_tag="VGB68_07085"
     CDS             1753..>3158
                     /gene="ftsH"
                     /locus_tag="VGB68_07085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEY0049031.1"
                     /translation="MSSKAKQILLWLMIISSALLFVWFIQRTQTKNPQELSFDQAITR
                     IEQKEVGEIFIKQDSVELTDKANPNNKFFTKLDTTDAPRESLLATAKTAGAVVKFEPV
                     SSNYFWIILIQALPFLLLVGFLVFTLRQMQAGGNKALSFGKSRAKLLNNQQKRITFKD
                     VAGVEEAKEELQEIIEFLKDPQKFQKLGGRIPKGVLMVGPPGTGKTLLAKAIAGEANV
                     PFFSISGSDFVEMFVGVGASRVRDLFEQGKKNAPCIIFIDEIDAVGRHRGAGLGGGHD
                     EREQTLNQLLVEMDGFESNDGVILVASTNRPDVLDPALLRPGRFDRRVVVGRPDVRGR
                     EGILKVHTRKIPLDEAVDVNIIARGTPGFTGADLANLVNEAALNAARYNKKVVTMSDF
                     EIAKDKVLMGAERRSMVISDAEKKLTAYHEAGHTLVGLKVPSADPVHKVSIIPRGMAL
                     GVTMQLPEADRHSYTKEY"
BASE COUNT          832 a          747 c          851 g          728 t
ORIGIN      
        1 gagaacctcc attgcctgtt ttactcaggt atgcacggct tgtcggaatc acaatggaag
       61 ttctggcgga tgataaactg gatttgccgg aatcgttgtt gaccggaaaa aaaagcgaaa
      121 aaataaaacg gtaaaaataa agtcggattt gccgattatg agaaattaat gggtagcgca
      181 agaccgaagc ccgccagact agctgagaaa ctgcttgccg ttcgtaatag cttgaatgga
      241 ggcgtgtctc aaaatgaatt aatccggttg atgcatttgg aaaatgagtt ggaacaagag
      301 cgaatttcaa aatatgaacg cggcgtgccg gagccgccgc tttacgttct tgtcgcttac
      361 gccgatgttg ctaatgttta cgtcgaagtt ttgattaaag atgatttgga tttgccggaa
      421 tctttgccga gccggaaaaa gagcgaagga ataaaacgtc aaaaataaag atgctttcgg
      481 cgacggttgc attcatattt atttgtggac gatattttat cggcgaatga aatcgaatga
      541 ataattttgt ccgcaatctg ctgaccgaat ggcgcaggct gaatctgccc tgtgaggatg
      601 aaactttcgt cgtcgccgtt tcgggcggcg ctgattcatt gagtttggcg ctggcgctcg
      661 acgatttaaa aaagcgaaag aaactgaatc tgcgttttgt cgtcgctcat ttcaatcaca
      721 atctgcgcgg cgcggaaagc gaggctgacg aaaaattcgt caaagattta gccgccgaat
      781 acgatttcga actggcgctg gggcgcggcg atatctcgga aaagggaaat ctcgaacagt
      841 cggcgcgcgt cgcccgttac gattttttga ccggcgtcgc cgaaaatctt cgggcgcgcg
      901 gagttttaac cgctcatacc atcaacgacc aggcggaaac ttttctgctg aatctgattc
      961 gcggaagcgg cattgaaggg ctttcggcga tgaagccgct tcgtttatta gaaagggaga
     1021 cgcgcagacg cggagacgcg cagacggaaa cccaagacct ttcttcaaac tcagcactca
     1081 gcactcagca ctcagcactt attctcgttc gtcctttgct cggctgggcg aagcgcagcg
     1141 atacggaaaa ttattgtcac gcggaaaaga ttgaatatcg ttacgattcg atgaacgagg
     1201 atttggcttt caggcgcgtg cggattcggc gcgtgctgct tccgcttttg caggatttta
     1261 atccgaaaat aatcgcgcgg ctggcggcga cggcggcttt gttgcgcgac gagcacgacg
     1321 agctcgaaaa acttgcaaat catcagctaa accaggcaaa caaagacgaa ctgccaaccc
     1381 gggatttgac aaatttatcg cccgccataa ggcgcagagt tttgcgggcg tggctggcga
     1441 gtcggcgcgg cgatttgcgg cggctggatt taaagcatct ggaagccgtc gagcgtttga
     1501 ttttcagccg gaaaagcggc agattcgtcg agcttccggg cggcgaaact gttgtcaaaa
     1561 caaacggaaa attagttttt aaataaagaa aggttgtaaa aaacgagccg cgccattaaa
     1621 atcaatactg gcgattatga aacaactttc acgccagaac acgcgggcaa caatttaaac
     1681 aacgtatcga acgaaacttt catcttaggg acacgtaatc atttaggatg aaagtagttt
     1741 ggaggctaaa agttgagttc taaagcaaaa caaattttat tgtggttgat gattatttcc
     1801 agcgcgctgc tgtttgtctg gtttatccag cgcacgcaga cgaaaaatcc gcaggaatta
     1861 tcctttgacc aagccatcac ccgaatcgag caaaaagaag tcggggaaat ttttatcaaa
     1921 caagattcgg ttgagctgac cgataaagca aaccctaata ataaattttt caccaagctg
     1981 gacacgaccg acgcgccgcg cgaatcgctt ctggcaacag ctaaaacagc gggcgcagtc
     2041 gtcaaattcg agccggtttc cagcaactat ttctggatta tcctgattca ggcgcttcct
     2101 tttctgctgc tggtcgggtt tttggttttt acgctgcggc aaatgcaggc gggtggaaac
     2161 aaagccttga gcttcggcaa atcgcgcgcc aagcttctga ataatcagca aaaacgaatt
     2221 acgtttaagg acgtcgcggg cgtcgaagaa gccaaagaag aattgcagga aatcatcgaa
     2281 tttctgaaag acccgcaaaa attccagaag ctcggcggac gcattccgaa aggcgttttg
     2341 atggtcggac ctccgggaac cggcaaaacg cttctggcga aagccatcgc cggcgaggca
     2401 aacgttccgt ttttctcgat ttccggttcg gactttgtcg aaatgtttgt cggcgtcggc
     2461 gcgagccgtg tgcgcgacct ttttgaacag ggcaagaaaa acgcgccgtg tatcattttc
     2521 atcgacgaaa tcgacgccgt cggtcgtcat cgcggcgccg gcttgggcgg cggacacgac
     2581 gagcgcgagc agactctgaa ccaattgctg gttgaaatgg acggtttcga atcgaatgac
     2641 ggcgtgattt tggtcgcttc gaccaaccga ccggacgttc tcgacccggc gctgcttcgt
     2701 cccggacgct ttgaccgccg cgtggtcgtc ggtcgtcccg acgttcgcgg acgcgaaggc
     2761 attctgaaag ttcacacccg aaaaattccg ctcgacgaag ccgtcgatgt caacatcatc
     2821 gctcgcggaa cgcccggatt taccggcgca gacctggcga acctcgtcaa cgaagcggct
     2881 ctgaatgcgg cgcgctacaa caaaaaagtc gtcacgatgt cggatttcga aatcgcgaaa
     2941 gacaaggttt tgatgggcgc ggaacgccgc agtatggtta tttccgatgc ggaaaagaaa
     3001 cttaccgcct atcacgaagc cggtcacacg ctcgtcggtt tgaaagttcc gtcggctgac
     3061 ccggttcaca aagtttcgat tattccgcgc ggaatggcgc tcggcgtgac gatgcagctg
     3121 cccgaagccg accgtcacag ctatacgaaa gaatattt
//
LOCUS       DASWNE010000351         1691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_294454, whole genome shotgun sequence.
ACCESSION   DASWNE010000351 DASWNE010000000
VERSION     DASWNE010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294454"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="VGB68_07090"
     CDS             complement(<1..969)
                     /locus_tag="VGB68_07090"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY0049032.1"
                     /translation="MQSLKFEASIAFASELDARDPLNGFREKFHIPVQPNGAAALYFT
                     GNSLGLQPKTARAYIEQELKDWETLGVEGHFHAKNPWMPYHEYLTGQMAKTVGALAVE
                     TVVMNSLTVNLHLLMVSFYRPTRQRHKIVIEAGAFPSDQYAVESQIKFHGLYRSAPTS
                     AAGGLYDEGQLNAKETFTNTLTRAAAADSDWQNSLIELKPRAGEACLRTEDIEKTIIE
                     NGDSIALILLGGVNYYTGQAFDMKRITEIGHRIGAVVGFDLAHAAGNIELHLHDWNVD
                     FAAWCSYKYLNAGPGAIAGCFVHERHAENFDLPRFAGWWGHDKETRF"
     gene            1200..1601
                     /locus_tag="VGB68_07095"
     CDS             1200..1601
                     /locus_tag="VGB68_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049033.1"
                     /translation="MFAFSVASAHAQQAVRQYQAKIPFDFNIGQKSYQAGNYVIKISR
                     LASNGIILSLEDDNNKKLQTVLVSETDTFAKNEPQLLFDVQENQRFLTKIYTQEIGLS
                     IGRRKSEKHNAAKKRQPASKPQVVAVTAHKE"
BASE COUNT          450 a          401 c          401 g          439 t
ORIGIN      
        1 aaaccgcgtt tccttatcgt gtccccacca gccggcaaat ctcggcaaat caaaattttc
       61 ggcgtgtcgc tcgtgaacaa agcagcccgc aattgcgccc ggtccggcgt ttaaatattt
      121 atacgagcac cacgccgcga aatccacatt ccaatcgtgc agatgcaatt cgatgtttcc
      181 agccgcgtgt gccaaatcga agccgacaac cgcgccgatt cggtgtccga tttccgtaat
      241 ccgcttcata tcaaaggctt gcccggtgta ataattcacg ccgccgagca aaatgagggc
      301 aatcgaatcg ccgttttcga tgattgtttt ttcaatgtct tcggttcgca gacaagcttc
      361 gcccgcgcgc ggctttaact cgataagcga gttttgccag tcggaatcgg ctgcggctgc
      421 gcgcgtcaga gtgtttgtaa atgtctcttt tgcgtttaat tgaccttcat catataaacc
      481 gcccgctgcc gaagtcggcg ctgacctgta aagcccgtga aattttatct gcgactcgac
      541 ggcgtattgg tcggacggaa acgcgcccgc ttcgatgacg attttgtgtc tctgccgtgt
      601 cgggcgataa aaagaaacca tcagcagatg cagattgacg gtcagcgaat tcatcacgac
      661 ggtttcgacg gcgagcgcgc cgaccgtttt tgccatctgt ccggtcagat attcgtgata
      721 cggcatccac ggattttttg cgtgaaaatg accctcgacg cccagagttt cccaatcttt
      781 taattcctgt tcgatatagg ctcgcgccgt tttcggttga agcccgagcg aattgccggt
      841 gaaatacaaa gccgccgcgc cgtttggctg tacgggaatg tgaaattttt ctctgaaacc
      901 attgagcggg tcgcgcgcgt ccagctcgga ggcgaaggcg attgaagctt caaattttaa
      961 tgattgcatc tgcatttata atgtttactt gaatttaacg gataaagaaa tgttttgaaa
     1021 gcggcaggct gaaaatttcg agccgaaaaa atttaattta tcgcagcctg atacttttcg
     1081 attggctttc gcgctttaac tgttgaaggc gaaaagccct cgaccaaaat tataattcat
     1141 aggaaacagc ggaggctata atgaaaaaat taattcgaat ttgtgcagtt ttaagcttag
     1201 tgtttgcatt ttcggtcgcg tcggcgcacg cgcagcaggc ggtcaggcag tatcaggcga
     1261 aaattccttt tgacttcaac atcggtcaaa aatcgtatca ggcgggcaat tatgtcataa
     1321 agatttcgag attggcttca aacggaatca ttctttcgct cgaagacgat aacaacaaaa
     1381 agcttcagac cgttttagtt tcggaaaccg acactttcgc caagaacgag ccgcaattat
     1441 tattcgatgt tcaggaaaat cagcgttttt taaccaaaat atatacgcag gaaatcggat
     1501 tgtcgattgg caggcgaaag tctgaaaaac acaacgccgc aaagaagcgg cagcccgctt
     1561 caaaaccgca ggtggtcgcc gtgacggcac ataaagaata agccgtcaaa attcgtatca
     1621 ctataaaaat agggaattta gcttgaatta gctaaattcc ctttttgttt gaggcagaaa
     1681 aataaaaccg c
//
LOCUS       DASWNE010000352        13094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_610151, whole genome shotgun sequence.
ACCESSION   DASWNE010000352 DASWNE010000000
VERSION     DASWNE010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13094
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610151"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..380
                     /locus_tag="VGB68_07100"
     CDS             177..380
                     /locus_tag="VGB68_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049034.1"
                     /translation="MNKVLAGTDIGGTKIAAALETPSGESVARDVCRAAAQMPDENEV
                     KIVSVGNQARKAYSNSYRMTYAA"
     gene            370..1581
                     /locus_tag="VGB68_07105"
     CDS             370..1581
                     /locus_tag="VGB68_07105"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEY0049035.1"
                     /translation="MQRKKNNFNRREFLTAIGGAGAAFSLAGLAASQTTKDEKTKEML
                     VYIGTYTSGKSRGKGIYIYKFNTENGALSPYKIVANVVEPSFLAIDKNRKYLYAVNET
                     VEYEGKKSGAVSAFAIDRKNGDLRFLNKQPSLGGAPCHITVSDNGKFVLVANYLGGNV
                     SVFPIENGGNLGAPIGLQQHRGSGANKQRQESAHAHSIILDNNNRFAFASDLGIDKIM
                     IYSFDDGDGKLEPNAAQAFYQTKAGAGPRHFKFHPDGKFAFVVNELDATVTSLEYDDK
                     RGTLKEIQTVSTLPADFSGAGTCADVHLSPDGKFLYGSNRGHDSIAAYKIDEKTGRLD
                     FIEHTPTQGRTPRNFAIDPAGKFLLAANQNSDSIVTFRIDEETGKLQPTGNKAIVPSP
                     VCLQLIPDFSA"
     gene            1729..5229
                     /locus_tag="VGB68_07110"
     CDS             1729..5229
                     /locus_tag="VGB68_07110"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEY0049036.1"
                     /translation="MKAIRRFFVFSLFAVTGFFLFSYAPSTIRQPVIAQSNNQDSPPV
                     SQATREAAYRANNLGVAQLEQFNYKEAAEEFRRALTLDSQMKIAQINLAIALFNLQDT
                     EGARQAAERAVSAAPERPQAHYILGLIARNQNRTDEAVVFFRRVLEIDPQDVGANVNL
                     GQIYIQQRKSAEAVAAFRLALASEPYNSTALYNLATALIRSSEREEGQKLIERFQALR
                     QSGAATSIGQNYLEQGRYAEAIASTGAETELVDKTTPKVVFQNVNVGLPVARAARRSP
                     GNTGNKLISNQPRRAATLFDYDNDGDLDLAQLAVSSPTMRLYRNDGGKFTDVTRAAGD
                     LAKPFATSGFGVIAGDYDNDGLTDLLVFGEGKAALFRNAGKGSFINNTAAAKLPEYSD
                     ISVSGAFVDADHDGDLDIFLAGFMSSVSPTRNALNQLLRNNGDGSFTDVSETAKIRSV
                     SSRAVAVVPTDYDNRRDVDFLVVNRDGKLNLFRNLRDGTFRDVAGEVGLNQSAEWTCA
                     AAGDFNKDAYTDFFFGKSGGAGVFAVSDGRGKFVLKDAPRGTENAASAQFLDYDNDGL
                     LDLIADTDKDLVVARNLGGEWTSADSSVFKVKTDFDNALVNSGQILSGDIDGDGDIDL
                     LAFGRGGNLHYISNVGGNANNSEIIRLQGRVSNRTGIGAKIDLRAGSLAQKLESYSAS
                     PAPAPADIHFGLGKREKPDAVRVVWTSGVIQAEVEFLQKTKAQTAKSFAPLKIEELDR
                     KPSSCPYLYTWNGEKFEFITDFLGGGEMGNWQKAGVYHYPDSDELVRIAPGKLKPKNG
                     KYEIRVTNELEEVLFVDYLKLVAVEHDAGGEVYPNEGLGIPTAGKRILYTTRGEETPL
                     SAVDDDGKNVLPEIEKLDRRFYDSFKSLNIRGYAAPHNLTLKLDDRKDYRGRTLLLLT
                     GWTDYAFSSDNLAASQSGKSLFLPKLQVKNRRGEWQTVIDNIGISVGRPQTLVVDLTG
                     KFLSDSREVRIVTNFKTYWDKIAVDASAPEDVKTIELQPSVANLRERGFSEEIRFGEM
                     ITANYEKVLNDGRWKYFSGNFTRTGDVKPLLNKIDDVFIISKTGDELVLSFDALPEPP
                     DGRKYTFLLYADGYSKEMDINSGSPEAVLPLPFKEMKKYPYGADERFPMTEEKQRIYD
                     EYTTRNARGFLPRLETALLK"
     gene            complement(5684..5860)
                     /locus_tag="VGB68_07115"
     CDS             complement(5684..5860)
                     /locus_tag="VGB68_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY0049037.1"
                     /translation="MNKKVRWGVLGAAKIALDKVIPAMRKGEWSEIVAVVSRDREKAR
                     EAARRLGIPKFYGS"
     gene            6151..7683
                     /locus_tag="VGB68_07120"
     CDS             6151..7683
                     /locus_tag="VGB68_07120"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEY0049038.1"
                     /translation="MGVIDSHNREKDGVSAGINKLRTGIEGLDRILEGGLPESNIYLV
                     EGEPGSGKTTLALQFLLEGIKQDETVLYVTLSETKNELENVAASHGWSLEGLNIYELV
                     PSGDTLKSESQYTIFHPSEVELNELTSSVLQEVERLNPKRVIFDSLSEMRLLARDPLR
                     YRRQILALKQYFAPRECTVLLLDDKTSNIVDLQVSSIVHGVVILEHLAVEYGAERRRL
                     RVSKLRGSRFRGGYHDFNIETGGVRVFPRLVSAEHVRNTNQETVKSGIAGLDELLGGG
                     LDRGTSTLIIGPAGSGKSSLAAQFAVAAAANGESAAVFIFDEIRETYVLRSTGIGTKI
                     QRYLDMGLVTIQKVDPAELSPGEFAHLIIESVKRNKTKVVIIDSLNGYMNAMPEERFL
                     TIQMHELLTFLNQQGVITIMIMAQHGFLGNAMTSPVDVSYLADTVLMLRYFESGGAVR
                     RALSVVKKRTGVHENTIRELDISGAGIRVGNALTGFQGVLTGVPTYSGKDIFVSDFDA
                     GK"
     gene            7670..10510
                     /locus_tag="VGB68_07125"
     CDS             7670..10510
                     /locus_tag="VGB68_07125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0049039.1"
                     /translation="MQANKGQNKREERILVLAPTGRDAELSARFLGEAGLTSKICGGI
                     GELCREISENAGAVFLTGEALEPETVALLTGTLQRQPPWSDIPIIVLTSGGGENPANA
                     DTLAELSEAGNVTLIERPVRMMTLGSALKSALRARRKQYEARDFLLAEIKSKEELRLS
                     EEQLRDARHRLESTLAAAEIGTWTWDCVNDKVTADKNLARMFSVSDEDAGGGALEKYL
                     QAIHPEDAEQVGKIIEKAIAEQSEFEAEYRLVSPQGTIRWIIARGIIERDSEGRALSL
                     PGVVVDITDRRRIEEALRDSDARLRLAIDISRTSTFDIDLQTDEVQTDDIGREIYGWE
                     ADTPLTFSRVQAHFHPDEREEVIRRVSDALAPTGSDEFEIEQRIIRTDGKIRWIRVRG
                     RAFFAGSGEHRTAVRCLGTYIDITESKKAEQALRESEERYRNLFNSIDEGFCVIEMIF
                     DDDGKAVDYLFLEINPTFEQQTGLENALGKRMRELAPNHEEYWFEIYGKVARTGEAIR
                     FESGAKALNRWFDFYAFRIGNFESRKVAVLFKDITKRKREEEEREKLLKSAQEARRTA
                     EEASRLKDEFLAVVSHELRTPLNAILGWTQMFRRQPFDSAITERAFETIERNARAQNQ
                     LIEDLLDVSRIISGKVRLDIQPVKLANVIEATLDSVRPAAEARDIALQTSLDEKIDAI
                     SGDPDRLQQIIWNLLSNAIKFTPRRGKVEVRLEQKNSHVRIIVADNGKGIEKDFLPFV
                     FDRFRQADSSSKRQYGGLGLGLSIVRQLTELHGGSVTVFSEGEGKGATFTVILPLSST
                     ARQQEYQQTPPADKNGFSNNAMNLAGSKVLLVDDDEDTRILLSMLLEQNSADVRAASS
                     VQEALEFLETDVFDLLISDIEMPHQDGYDLIKFVRQMPQKSDIPAIAVTAYTRNEDSR
                     RAIACGYDRHVPKPVESGNLLTIIESLVKRKS"
     gene            10600..11844
                     /locus_tag="VGB68_07130"
     CDS             10600..11844
                     /locus_tag="VGB68_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049040.1"
                     /translation="MAKKPGIFPTFFISGFECSTFLWKDKRRRNLVAETQHDKCVRDD
                     YRILRDLGIAVAREGIPWALVDKNGQYDFSPIDPMIEAMNETRIIPIWDLCHYGYPDD
                     LDPFSEEFTGRFADYARAAARYVTPRVRGPHFFTPVNEITFFSFIGGEWGWTAPYRNT
                     REDRFKLRLALCRAAIAGVLAIREIEPQARMVHIDPLVQVVAPRDRPDLAEAARQETY
                     VDTFLAWDILCGKEHAELGGSPEILDIVGANNYSFGQMEYREQGPHAALAPEDDRIKP
                     LCDLMKLVWERYRRPMIIGETSGLGGGRAAWLKDVVEESLAAVNEGMDLHGICLFPAV
                     DMPDWHTGEWLHNGICDLEECEGHLRRVPSENYVNELRRWQKELNRVTALDKDPFSDP
                     VELQDVIDAARRLRPKPDKDWS"
     gene            11906..12685
                     /locus_tag="VGB68_07135"
     CDS             11906..12685
                     /locus_tag="VGB68_07135"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY0049041.1"
                     /translation="MKSFDTSGFIMTEVAYSARTRLSEHSHENAHFCFVLQGTYTESH
                     NKQELQCEPFTLTFRPPGEVHEDKFHSQDVRVFTIEIPPRWMERLEQDSIYLGRSINF
                     KDGFVTQLSERIVREFHRMDTAAGLIIEGLTLEIMAETARHSAQSVERTIPYWLRRAK
                     DLLHARFTENLTLEQIAREVGIHPVHLSSVFRQKYHCTVGEYIRQLRIEYACREIAKG
                     EMPLAIIALEAGFANQGHFSGTFKRLTGLTPAAYRNFSRQS"
BASE COUNT         3230 a         3357 c         3547 g         2960 t
ORIGIN      
        1 cggcgctgga aatcgtcgtc gtatcgggag ttaagaaatt aatcaatgcc gccgcaaccg
       61 gcaatgtgac gtcaggcgtc ggcgcgaatt gaaagagcta aatcttctga aaagaagaaa
      121 cgcggctgat ttgaaagtct gcaatttatc gcctgaatca taacgaaatc gcgcaaatga
      181 ataaagtttt ggctggaaca gacatcggcg ggacgaaaat cgccgcggcg ctcgaaacgc
      241 cgtccggcga gagcgtcgcg cgcgacgtct gccgcgcggc ggcgcaaatg cctgatgaaa
      301 acgaggttaa aatagtttcc gtcggcaacc aggcgcgaaa ggcgtattca aattcttatc
      361 gaatgactta tgcagcgtaa aaaaaacaat ttcaacaggc gcgaattttt gacggcaatc
      421 ggcggcgcgg gcgcagcttt ttcgctcgcc ggtttggctg cttcgcaaac gacaaaagac
      481 gaaaaaacaa aagaaatgct cgtttacatc ggcacttaca cttccggcaa aagccggggc
      541 aaaggcatct acatctataa atttaatacg gaaaacggcg cgctgagtcc ttataaaatt
      601 gtcgcaaacg tcgtcgagcc gtcatttctg gcgattgata aaaacaggaa atatctctac
      661 gccgtcaacg aaaccgtcga atacgaagga aaaaaaagcg gcgcggtcag cgcgtttgcg
      721 attgaccgga aaaacggcga tttgcgtttt ttgaataaac agccgagtct cggcggcgcg
      781 ccgtgtcata ttacggtcag cgacaacggc aaattcgtgc tggtcgccaa ttacctcggc
      841 ggaaacgtgt cggtttttcc aatcgaaaac ggcggaaatc tcggcgcgcc gattggttta
      901 cagcagcacc gcggctcggg cgcgaacaaa cagcgacagg aatcggcgca cgcgcactcg
      961 ataattttag ataacaataa ccgttttgcc ttcgccagcg atttgggcat cgataaaata
     1021 atgatttaca gtttcgatga cggagacggg aagctggaac cgaacgccgc gcaggcgttt
     1081 taccagacaa aagccggcgc cggaccgcgg cattttaagt ttcacccgga cggaaaattc
     1141 gcctttgtcg tcaacgagct cgacgcgacc gtcacgtcgc tcgaatacga cgacaagcgc
     1201 ggaacgctca aggaaattca aaccgtttcg acgcttcccg cagatttttc cggcgcgggc
     1261 acctgcgccg acgtacacct ctcgcccgac gggaaatttc tttacggctc gaatcgcggt
     1321 cacgacagca tcgccgctta caaaatcgac gagaaaacgg gcagactgga ttttatcgag
     1381 cacacgccga cgcaagggcg gacgccgcgc aattttgcga ttgacccggc gggcaaattc
     1441 ctgctcgcgg caaatcagaa ttccgattca atcgtcacgt ttcgcatcga cgaggaaacc
     1501 ggaaaattgc agccgaccgg aaataaagcg attgttccgt cgccggtttg tctgcaatta
     1561 ataccggact tttcagctta aaaaaagccg tttgcgccgt gctgcgagcc ggatgaaaat
     1621 aaccggtttc gttgaattgc caaagtaaaa tcaatcgaat aatttacatt cgattgattt
     1681 tactttctcc ggtcaagcgc tttcgagcga agttgttata atcgttttat gaaagcaatc
     1741 cggcgttttt ttgtcttcag tttgtttgcc gtcacaggct tttttttatt ttcatacgcg
     1801 ccctcgacca ttcgacagcc ggtaatcgcc caatcaaata atcaagattc tccgcccgtt
     1861 tcgcaggcga cgcgcgaggc tgcatacagg gcgaataatc tgggcgtcgc gcagctcgaa
     1921 caatttaatt ataaagaagc ggcagaagag tttcgccgcg ctttaacgct cgattcgcaa
     1981 atgaaaatcg cgcaaatcaa tctcgccata gcgcttttca atctgcagga taccgaaggc
     2041 gcgcggcaag ccgccgaaag agccgtcagc gcagcgccgg aacgcccgca ggcgcattat
     2101 attctcggat tgattgccag aaatcaaaat cgcaccgacg aggcggtcgt ttttttccgg
     2161 cgcgttctgg agattgaccc gcaggatgtc ggcgctaacg tcaatctcgg tcagatttac
     2221 attcagcagc gcaaatccgc cgaagccgtc gccgcgtttc gccttgcgct cgcctccgag
     2281 ccttacaatt cgacggctct ctacaatctg gcgaccgcgc tgatacgcag cagcgagcgc
     2341 gaggaaggac aaaaattgat tgagcgtttt caggctctca ggcagagcgg cgcggcgacg
     2401 agcatcgggc agaattatct cgaacaggga cgctacgccg aagcgattgc ctcgaccggc
     2461 gcggaaaccg aactcgtcga taaaactacg ccgaaagtcg tctttcaaaa cgtaaacgtc
     2521 ggcttgccgg tcgcgcgcgc cgctcgacgc agcccgggaa ataccggaaa taaattgatt
     2581 tcaaatcagc cccgccgcgc cgcgactctt ttcgattacg ataacgacgg cgatttggat
     2641 ttggcgcagc ttgccgtcag ttcgccgacg atgcggcttt accgcaatga cggcgggaaa
     2701 tttactgacg tgacgcgagc ggcgggcgat ttggcgaagc cgttcgcgac gtccggtttc
     2761 ggcgtcattg cgggcgatta cgataacgac ggattgacgg atttactcgt tttcggcgaa
     2821 ggaaaagccg cgctttttcg caatgccggt aaaggcagct ttataaataa taccgccgcc
     2881 gcaaaactgc cggaatacag cgacatctcg gtgtcgggcg cgttcgtcga tgccgaccac
     2941 gacggcgacc tcgacatatt tctcgcgggt tttatgagca gcgtcagccc gacccgaaac
     3001 gctctgaatc aactcctgcg caacaacggc gacggcagct tcacggacgt ttccgaaacg
     3061 gcgaaaatcc ggagcgtttc gagccgggcg gtcgccgtcg tgccgaccga ttacgataat
     3121 cgccgcgacg tcgattttct ggttgtaaat cgcgatggga aattgaatct ctttcgcaac
     3181 ctgcgcgacg gcactttccg cgacgttgcc ggcgaagtcg ggttaaatca aagcgccgaa
     3241 tggacttgtg ccgccgccgg agatttcaat aaagacgctt acaccgattt ctttttcggc
     3301 aaatcgggcg gcgcgggcgt ttttgccgtc tccgacgggc gcgggaaatt tgttttaaaa
     3361 gacgcgccgc gcggaacgga aaacgctgct tccgcgcagt ttctcgatta cgacaacgac
     3421 ggcttgctcg atttgattgc cgatacggat aaggatttgg tcgtcgcccg caatctcggc
     3481 ggcgaatgga caagcgcgga ttcttccgtt ttcaaagtaa aaaccgattt cgataacgct
     3541 ttagtcaatt cggggcaaat actgtcgggc gacatagacg gcgacggcga tatcgatttg
     3601 ctggcgttcg ggcgcggcgg caatttgcat tacatcagca atgtcggcgg caacgcgaat
     3661 aattcggaaa tcatacgtct tcaaggtcgc gtcagcaacc ggaccggcat cggcgcgaaa
     3721 atcgatttgc gcgcgggcag cctcgcgcaa aagctcgaaa gctattccgc cagccccgcg
     3781 cccgcgcccg ccgatattca tttcggactc ggaaaacgcg aaaaacccga cgccgtgcgc
     3841 gtcgtctgga cttccggcgt gattcaggcg gaagtcgaat ttctccaaaa aacgaaagct
     3901 cagacggcga aaagctttgc gcctttgaaa atcgaggaac tcgaccgaaa gccttcttcg
     3961 tgcccgtatc tatacacctg gaacggcgaa aaattcgagt ttatcaccga ttttctgggc
     4021 ggcggcgaga tgggcaactg gcagaaagcg ggcgtttatc attatcccga ttccgacgag
     4081 ctcgtgcgca tcgcgcccgg taaattaaag ccgaaaaacg gaaaatacga aattcgcgtg
     4141 acgaacgagc tggaagaagt tttgtttgtc gattacctga agctcgtcgc cgtcgagcac
     4201 gacgccggcg gcgaagttta cccgaatgaa ggcttgggaa tcccgaccgc cggaaagcgg
     4261 attttatata cgacgcgcgg cgaggaaacg ccgctttccg ccgtcgacga tgacgggaaa
     4321 aatgttttgc cggaaataga aaaactcgac cggcgatttt acgattcgtt caaatcgctg
     4381 aacattcgcg gctacgccgc gccgcacaat ctgactttga agctggacga cagaaaagat
     4441 tatcggggca gaacgctcct gcttttgacg ggctggacgg attacgcttt ttcgtccgac
     4501 aatctcgccg cttcgcaaag cggaaaatct ttgtttttgc cgaaactgca ggtcaaaaac
     4561 cggcgcggcg aatggcaaac cgtgattgac aatatcggaa tttccgtcgg tcgcccgcag
     4621 actctggtcg tcgatttgac cggaaaattt ttgtcggatt cgcgcgaagt tcgcatcgtc
     4681 acgaatttta aaacctattg ggacaaaatc gcggtcgacg cgtccgcgcc ggaagatgtc
     4741 aaaacaatcg aattacaacc gtcggtcgcg aatttacgcg agcgtggatt ttccgaagaa
     4801 atcagattcg gcgagatgat tacggcaaat tacgaaaaag ttttaaacga cgggcgctgg
     4861 aaatattttt ccggcaattt tacccgaacc ggcgacgtca agcctttatt aaataaaatc
     4921 gacgacgttt tcatcatctc gaaaacgggc gacgagctgg ttttgagctt cgacgccctg
     4981 ccggaaccgc ccgacggcag aaaatacacg tttctgcttt acgccgacgg gtacagcaag
     5041 gaaatggaca tcaattccgg cagccccgaa gccgttttgc cgctgccgtt taaagagatg
     5101 aaaaaatatc cgtacggcgc ggacgagcgt tttccgatga cggaagaaaa acagcgaatc
     5161 tacgacgaat acacgacgcg caacgcgaga ggctttctgc cgcgcctcga aacggctttg
     5221 ttgaaataaa cggcgcggcg cgtttgttcc ggcacgattg ggcggcttta tcttcgattt
     5281 ttgcggcgag ccgtcgaaaa cgccggtttt tacccgttgt tttattcgtt ttttcggatt
     5341 tccgcgcgtt agagacgatt attcgccatc agagacgatt attcatccgt tagaagcgat
     5401 tattcacctg ttagaggcga ttgttagtct gttagagttg attgttagcc cgttgacgat
     5461 gattatcact cgttttagac gattattcac tcgttttagg cgattgttag cccgttttag
     5521 gttgttgtca cctcgtttta agtcgttgtc atctcgtttt aagtcgttat cagttcgttt
     5581 taagtcgttg caagttcgtt ttaagtcatt gtaagttcgt ttcaggcgat tttcatctcg
     5641 tattaaccta aattaatttc tgaatcgttt cccattttcc cgtttatgaa ccgtaaaatt
     5701 tcggtattcc cagacggcgc gccgcttccc gcgctttctc ccggtctctg gaaacgacgg
     5761 cgacaatttc agaccattcg cctttccgca tcgccggtat aactttatcc agggcaattt
     5821 tcgccgcgcc gagaacgccc cagcgaacct ttttattcat caatttattc tccgccgtta
     5881 aatcttataa ttagttcctt acgacttcaa aagtccgccg ggcggttgcc gagccgaagc
     5941 cgtcaaccga gccgtcgcgc gtaaagtcgt caaccgctcc ggcaggactc ggcgagatac
     6001 ggcggcgaaa cgcgaatttg ataaatgcag ttcatttctt aaccccggaa ttgctttcag
     6061 cgctgaaaat aaatttctgt aaccagttgt tctatgctat ttttgtattt atacacgaat
     6121 agataaggat ataaattgaa ttgatatttt atgggcgtta tagattctca caatcgagaa
     6181 aaggacggcg tttccgccgg aataaacaag ttaagaacgg gtattgaagg tttagacagg
     6241 attttggaag gcggtttgcc ggaaagcaat atatacctgg tcgagggcga gccgggttcg
     6301 ggaaaaacga ccctcgcgct tcaatttttg ctggaaggca tcaaacagga cgaaacggtt
     6361 ctttacgtaa ctctttccga aaccaaaaac gaattggaaa acgtcgccgc ttcgcacggc
     6421 tggtctctgg aaggcttgaa tatctatgaa ttggttccgt ccggcgacac tctcaaatcc
     6481 gagtcgcaat acaccatttt tcatccgtcg gaagttgaat taaacgagct gaccagctct
     6541 gttctgcagg aggtcgagcg ccttaacccg aagcgagtca ttttcgattc gctctcggag
     6601 atgcgcctgc tggcgcgcga cccgcttcgc taccggcggc agattctcgc gctcaaacaa
     6661 tattttgcgc cgcgcgagtg caccgttctg ctgctcgacg ataaaacctc gaatatcgtc
     6721 gatttgcagg tcagctcgat tgttcacggc gtcgtaatac tggagcatct ggcggtcgaa
     6781 tacggcgcgg aacgtcgtcg cctgcgagtc tcgaaactgc gcggctcgcg ttttcgcggc
     6841 ggctaccacg actttaatat cgaaaccggc ggcgtgcgcg tttttccgcg tctggtctcc
     6901 gccgagcacg ttcggaatac gaatcaggaa acggtcaaaa gcggcatcgc cggattggac
     6961 gagctgctcg gcggcgggct cgaccgcgga accagcactt tgattatcgg tcctgccggt
     7021 tccggaaaat cgtcgctggc ggcgcaattt gcggttgcgg cggcggcgaa cggcgaaagc
     7081 gcggcggttt ttattttcga cgaaattcgg gaaacgtatg tcctccgctc gaccggcatc
     7141 gggacaaaaa tacagcgata tctcgatatg ggtctggtga cgatacagaa ggtcgatccg
     7201 gcggagcttt cgcccggcga gttcgcccat ttgattatcg aatcggtaaa gcgaaacaaa
     7261 accaaagtgg tgattatcga cagcctgaac ggttatatga acgcgatgcc ggaagagcgt
     7321 tttctgacca ttcagatgca cgagctttta accttcctga accagcaggg cgtcatcacg
     7381 ataatgatta tggcgcagca cggttttctg ggaaatgcga tgacgtcgcc ggttgacgtc
     7441 agttatctgg cggatacggt tttgatgctg cgctattttg aatcgggcgg cgctgtgcgg
     7501 cgcgctttgt ccgtggtcaa aaagcggacg ggcgtgcacg aaaatacgat tcgggaattg
     7561 gacatctcag gcgcgggcat tcgcgtcggc aatgcgctta ccggttttca gggagtttta
     7621 acaggggttc cgacttatag cggaaaagat atatttgttt cggattttga tgcaggcaaa
     7681 taaaggacag aacaagcggg aagaacgaat attggttttg gcgccgaccg gcagagatgc
     7741 cgagctgagc gcgcgctttt taggcgaagc cggtttgact tctaagatat gcggcggcat
     7801 cggcgagctg tgccgcgaaa tttcggaaaa tgcgggagcc gtttttctga cgggcgaagc
     7861 gctggagccg gaaaccgttg cgcttctgac cggcactcta cagcgtcagc cgccgtggtc
     7921 ggatattccg attatcgtcc tgaccagcgg cggcggcgaa aatcctgcca atgccgacac
     7981 tctcgccgag ctcagcgaag ccggaaacgt caccttaatc gagcgccccg tgaggatgat
     8041 gacgctcggc agcgcactca agtcggcgct cagggcacgc cgcaaacaat acgaggcgcg
     8101 ggatttcctg ctcgccgaaa ttaagtcgaa agaagagctg cgactgagcg aagagcaatt
     8161 gcgcgatgcg cgccaccgtt tggaatcaac gctcgccgcc gccgaaatcg gaacctggac
     8221 gtgggattgc gttaacgata aagtaaccgc cgacaaaaat ttagcacgga tgttttccgt
     8281 ctctgacgaa gatgccggcg gcggcgcgct tgaaaaatat ttgcaggcaa ttcatccgga
     8341 agacgccgaa caagtcggta aaattattga gaaagccatc gctgaacagt ctgaattcga
     8401 ggctgagtat cgtcttgttt cgccgcaggg gacgattaga tggattatcg cgcgcgggat
     8461 tattgagaga gattccgagg gtcgtgccct gagcctgccc ggcgtggtcg tcgatattac
     8521 cgaccgccgc cggattgagg aagctctgcg cgattcggac gcccgattgc ggctcgccat
     8581 agacatttca cgaaccagca cttttgatat agatttgcag accgacgagg tgcagaccga
     8641 cgacatcggg cgtgaaattt acgggtggga agcggacacg ccgcttacat tcagccgggt
     8701 tcaggcgcat tttcatcccg acgagcgcga ggaagtaatc cggcgcgtgt cggatgcctt
     8761 agctccgaca ggttcggatg aatttgaaat cgaacagcgc atcattcgca ccgacggcaa
     8821 aatacgatgg attcgcgtgc gcggaagggc attttttgcg ggctccggcg agcaccgcac
     8881 cgccgtgcgc tgtctcggca cgtatatcga cattaccgaa agcaaaaaag ccgaacaagc
     8941 cctgcgcgaa agcgaggagc gctaccgcaa tttatttaac tcgattgacg aaggcttttg
     9001 cgttatcgag atgatatttg atgacgacgg caaagcggtc gattatcttt tcctggaaat
     9061 taatccgaca ttcgagcagc aaaccggact cgaaaatgca ttgggaaaaa gaatgcgcga
     9121 attagcgccg aatcacgaag agtattggtt tgaaatttac ggcaaggtcg cccggacggg
     9181 cgaagcgatt cgttttgaaa gcggggcgaa agcactgaat cgctggttcg atttttatgc
     9241 ttttcggatt ggaaatttcg aaagccgaaa ggtcgccgtt ctttttaaag acatcaccaa
     9301 gcgcaagcgg gaagaagaag aacgcgagaa actgctgaaa agcgcgcagg aagcccgccg
     9361 gacagccgaa gaagcgagcc gcctgaaaga cgagtttctg gcagtcgttt cgcacgaact
     9421 gcgcacgccg ctcaacgcga ttctaggctg gacgcagatg tttcgccgcc agccgttcga
     9481 cagcgccatc accgagcgcg cttttgaaac gatcgagcgc aatgcccgcg cgcaaaatca
     9541 attgattgaa gatttgctcg acgtatcgcg gattatcagc ggcaaagtgc gactcgatat
     9601 tcagcccgtt aaattggcaa acgtcatcga agcgacgctc gattccgtcc gaccggcggc
     9661 tgaggcgcgc gatattgctc tgcaaacgag tctcgacgaa aaaatcgacg cgatttccgg
     9721 cgaccccgac cgtttgcagc agattatctg gaacctttta tcaaacgcca tcaagtttac
     9781 gccccgcagg ggaaaagtcg aggtgcggct cgagcagaaa aattcgcacg tcaggataat
     9841 cgtcgccgac aacggcaaag gaatcgagaa ggattttctg ccgttcgttt tcgaccgttt
     9901 tcgccaggcc gacagctcca gcaaaagaca atacggcggc ttgggtctgg gattatcaat
     9961 cgtccgccag ctgaccgagc ttcacggcgg ctcggtcacg gttttcagcg agggtgaagg
    10021 aaaaggcgcg acctttaccg tcatcctgcc gctgtcgagc accgcgcggc aacaggaata
    10081 tcagcaaacc ccgcccgccg ataaaaacgg cttttcaaac aatgctatga atctggcggg
    10141 gtctaaagtt cttttagtcg atgatgacga agacacgcga attctgttat cgatgcttct
    10201 ggagcagaac tcagccgacg ttagagccgc gtcgtccgtt caggaagctt tagaatttct
    10261 cgagaccgat gttttcgatt tgcttatctc cgacatcgaa atgccgcatc aggacggtta
    10321 tgatttaatt aaattcgtca gacagatgcc gcaaaaaagt gatattccgg cgatagccgt
    10381 gacggcatat acgcgaaacg aagacagccg gcgcgcgatt gcctgcggct atgaccggca
    10441 cgttccgaaa ccggtcgagt ccggcaattt gctgacgata atagaaagtc tggtcaagcg
    10501 gaaaagttaa agcggttttc cttgtagcct ttatctttcc tttttagaaa aggttcgggt
    10561 taatataagt ttagaaaatg ttcgagttaa tataagcgta tggcaaaaaa accgggaatt
    10621 ttcccgacat tttttatttc cggttttgaa tgttccactt ttctctggaa agataagcga
    10681 cgccgcaatc tggtcgccga aacgcagcac gataaatgcg tccgcgacga ttacaggatt
    10741 ttgcgcgacc tcggaatcgc cgtcgcgcgc gaaggaattc cgtgggcgct ggtcgataaa
    10801 aacggacagt acgatttttc cccgattgac ccgatgattg aggcgatgaa cgaaacacgg
    10861 ataattccaa tctgggattt gtgtcattac ggctatccgg acgatttaga ccctttttcc
    10921 gaagaattta ccgggcgttt cgccgattac gcccgcgccg ccgcccgcta tgtgacgccg
    10981 cgcgtgcgcg gtccgcattt ttttacgccg gttaacgaaa tcacattttt ttcatttatc
    11041 ggcggcgaat ggggctggac tgcgccttac agaaatacca gagaagaccg ttttaaactg
    11101 cgtctggctt tatgccgagc cgcgattgcg ggcgttttgg cgattcggga aatcgagccg
    11161 caggcgcgaa tggttcacat cgatccgctc gtgcaggtcg tcgcgccgcg cgaccgtccg
    11221 gatttggcgg aagccgcccg tcaggaaact tatgtggaca cttttctggc gtgggatatt
    11281 ctgtgcggaa aagagcacgc cgagctcggc ggctcgcctg aaattctgga tattgtcggc
    11341 gctaataatt attctttcgg acagatggaa taccgcgagc aaggtcctca cgccgcgctc
    11401 gccccggaag acgaccgcat caagccgctt tgcgatttga tgaagctggt ctgggagcgc
    11461 taccgccgac cgatgataat cggcgaaacc agcgggctcg gcggcgggcg cgcggcgtgg
    11521 ctcaaagatg tcgtggaaga atcgctcgcc gccgtcaacg aaggtatgga tttacacggc
    11581 atttgtctgt ttcccgccgt cgatatgccg gactggcaca cgggcgaatg gcttcacaac
    11641 ggaatctgcg atttggaaga atgcgaaggg catctgcgcc gcgtcccgag cgaaaattat
    11701 gttaacgagc tgcgccgctg gcaaaaggaa ttaaaccgcg tcaccgcgct cgataaagac
    11761 cctttcagcg acccggtcga attgcaggac gtcatcgacg ccgcccgccg gcttcgaccg
    11821 aaacccgata aagactggag ttaaccggct tttaatccga gatatgttta aaaaattagc
    11881 gccaggctgc tcaaatggaa aagttttgaa gagtttcgac acgtcgggct ttattatgac
    11941 cgaggtcgcg tattcggcac ggacgaggct ttcggagcat tcacacgaaa acgctcattt
    12001 ttgttttgtt ctgcaaggca cttacaccga gtcgcacaac aaacaggaat tgcagtgcga
    12061 gccgtttact ttaacctttc gcccgcccgg cgaagttcac gaagacaaat ttcatagtca
    12121 ggacgtgcgg gtttttacta tcgaaatccc gccgcgatgg atggaaaggc tcgagcagga
    12181 ttcaatctat ctgggtcgct caatcaattt caaagacggc tttgtcacgc agctttccga
    12241 gcggattgtc agggagtttc atcgaatgga caccgccgcc ggtttgataa tcgaaggctt
    12301 gacgctcgaa ataatggctg aaaccgcgcg tcactcggcg cagagcgtcg agcggacgat
    12361 tccatactgg ctcaggcggg cgaaggattt actgcacgcg cgcttcaccg aaaatttaac
    12421 gctcgaacaa atcgcgcggg aagtcggcat tcatccggtt catctttcga gcgtgttccg
    12481 gcaaaaatat cactgcaccg tcggcgaata catccggcag ttaagaatcg aatacgcctg
    12541 ccgcgaaatt gccaaaggag aaatgccgct ggcgattatc gctctggaag ccggatttgc
    12601 caatcaaggt catttttccg gcacttttaa acgcctgacg ggcttgacgc ccgccgccta
    12661 cagaaatttt tcccgccaat cttaatccgg tataaaaacc ttaaatttac gtaagacgcg
    12721 ccccgagaaa caattctaaa atgcgaataa aggacgagcg tctttattcg ccgatttcat
    12781 tttgtttcaa aaaattatga aaagaaaaaa gtgaagctcg agccgggcgg tggcggcgga
    12841 aaggctcgcg caatgacgat tgccaatcct caagacgctg tcacggcaag cagccggtta
    12901 taaggattta tacttctgag aatcgagata aaagcgagcg gtatttatat gaaacttacg
    12961 aaaaatattt tcaaatacgg cgccggtttt gtgctgctgt ttcttctgtg cgccgcaccg
    13021 ggacgcgcgg aaattacggc aatcagagcc ggaaaactgg ttaaccccga aaccggcacg
    13081 accgccgtca atca
//
LOCUS       DASWNE010000353         7454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_436101, whole genome shotgun sequence.
ACCESSION   DASWNE010000353 DASWNE010000000
VERSION     DASWNE010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7454
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436101"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1806)
                     /locus_tag="VGB68_07140"
     CDS             complement(<1..1806)
                     /locus_tag="VGB68_07140"
                     /inference="COORDINATES: protein motif:HMM:NF025253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEY0049042.1"
                     /translation="MFALFIACAVSIAAQTTAFTYQGRFTDSTVTQPTNGTYTMKFRL
                     YDALANGNQVGSELSSSVAVTNGIFTTSLDFATGPFAAGQSLWLEIQVGATTLAPRQK
                     IESVPYAVRSISAGSADLLSSLCNPCVTSAQIVSLDGSKITGTVLNATNATNATNAIN
                     ATNAANATNATNATNATTAVTISGIITTAQVSGDVELAPSVQQTTSTTNHLINLKLVG
                     ARNLGTAGQDSLLSLSASGTYWSGGFTQNFDKERFRVENDGSILAVSDYNSGCCGGIP
                     IEGSGTRFMWFGAKAALRAGSINGTQWDDANIGLYSTAFGENVRALGDNAIVVGKNSV
                     AANTGTVALGEGHTATGANSVALGYGASTSTLAGAPRTGTFVFSDRSVPLTYTFNSTS
                     PDTATQFHPTVTNSFNVRAINGSFFYTNTALTTGFIFNSTTATMTLANSKLTMASDGS
                     TTLTSNSGGSAGVTLPAGGGGWNNLSDRNMKENFAAVDSREILRGVLGLPVSTWNYKT
                     QNPSIRHIGVMAQDFSSVFKVGENDKTISTIDPDGIAFAAIQGLYAELKDRDAKIERQ
                     QQQLTQFQRQLDQQQMTIDALKKLVCAGKSKAGVCK"
     gene            2024..2710
                     /locus_tag="VGB68_07145"
     CDS             2024..2710
                     /locus_tag="VGB68_07145"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0049043.1"
                     /translation="MKIPESAKEEFDPREHTKELAAEFLEKGDATGWFDALYKEAEGN
                     HEHIPWADLKPNKFLARFAEKTNLQGGGRRALVVGCGLGDDARFLHDLGFKVTAFDIS
                     STAIEWAKKLHAETSIEFFVADLFNPPQHWRERFEFVLEVYTIQPLPLEMREKVIDAI
                     AQFVAENGKLVVVTRGREDDEQPLELPWALSRKDLSRFEFNALKQTHFEEMPGDEEEP
                     VRRFVVEYLR"
     gene            complement(2772..4109)
                     /locus_tag="VGB68_07150"
     CDS             complement(2772..4109)
                     /locus_tag="VGB68_07150"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0049044.1"
                     /translation="MENIKLLLALFYRPLSAMSDIIDNGNWFFAAVAVFLVSLAFFST
                     VNSKLEKAYHVPNLYEFYQPDYSTTNESAPAAEAEYERAVAEYNRAMDARQKVPFAGD
                     NFFRFFSFEPSGFFRPVLTISCFYVPATILLLCIFGNIGNFGVVVRRDYAALATCSLM
                     AWAAAHLPFAVAGIALYSPAVSPLVYFAFWLASGILFGALMIFALRTVFGVNYGAAIV
                     TVCLSWLAFSAGMYVFRFVSPWLFSPFLALYAYMYFGGYLRGEVSGFSDAFRQKQNFK
                     RFLQNATVNPRDADAHVQLALVYLQRRQETKALEHLNRAVEIDPSEPDANYELGKIAR
                     QKGDLQQALNHFSTVVEQNDKHALSEIWREIGATYLAANMLEEARAALEKYVERRSVD
                     AEGLYHLGKVLKAQGETERAREMFRQAIESAQTSPDYRRRELRYWSKLAEKEI"
     gene            4269..5681
                     /gene="fumC"
                     /locus_tag="VGB68_07155"
     CDS             4269..5681
                     /gene="fumC"
                     /locus_tag="VGB68_07155"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEY0049045.1"
                     /translation="MSEATKQDIKTRIETDSMGEIAVASDKYWGAQTERSLHHFNIGF
                     DTMPREMIRALGILKKAAAQVNHELKADKLTKEKLDLIVRAADEVIEGRLDAHFPLRV
                     WQTGSGTQTNMNANEVISNRAIEIAGGEMGSKKPVHPNDDVNMSQSSNDTFPTAMYIA
                     AAERLDALIPRVREVYEAIDAKAKEFADVVKIGRTHLQDATPLTVGQEFGGWASLIER
                     DIERLKLVMSGLLDLAIGGTAVGTGLNSHPEFAEKAAAKIAELTGLPFKAHPNKFAAL
                     SAHDEIVFASGALKTLAGSLMKIANDVRWLGSGPRCGIGELVLPENEPGSSIMPGKVN
                     PTQSEAMTMVAAQVLGNDAAIGFAGSQGNFELNVFKPVMIHNFLHSVRLLHDACHGFV
                     EFCINGIQLNRAQIDEYLNDSLMLVTALNQHIGYDNAAKIAKNAHKKGLTLKESAIEL
                     GLLTGEKFDELVKPEDMTHP"
     gene            5750..6424
                     /locus_tag="VGB68_07160"
     CDS             5750..6424
                     /locus_tag="VGB68_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049046.1"
                     /translation="MKKIFLFVLLLSAPAFAQVKFEKGNASKNYNARIEVERCAEGTC
                     DGKLKIELFKKSAAKPFQVLHLDQTEFAVEEAKQTNARRRYDYQSMIFFEDYNFDGIE
                     DLSIRDGNNGAYNGPSYQIYLFSPPRGKFVHSPALTRLAQSEYLGMFEVDKKKRVIRV
                     ASKGGCCMHRKEEFRVVNNRLVKVFEIYEEVEFWKENVNRVRIETSRLIKGRWQTRVR
                     YEKIKD"
     gene            complement(6521..7012)
                     /locus_tag="VGB68_07165"
     CDS             complement(6521..7012)
                     /locus_tag="VGB68_07165"
                     /inference="COORDINATES: protein motif:HMM:NF020904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEY0049047.1"
                     /translation="MSEDFDLEFLPGTEAGEFDDRYVKETEDMLRLKFEPDYLAFLEK
                     HNGGVPRKQFFPLDDNVKVVERFLSFVPDYEDNEEFGYYDVAVVWSQISDRLNEYLVP
                     FAIIYPGDFLCFDYEGGGQAKIVLWDHDLSGEMKPYTIPVVDNFRQFLSMLTDDEEGG
                     KDS"
     gene            complement(7034..>7454)
                     /locus_tag="VGB68_07170"
     CDS             complement(7034..>7454)
                     /locus_tag="VGB68_07170"
                     /inference="COORDINATES: protein motif:HMM:NF024053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY0049048.1"
                     /translation="IINASKPWSWKMLGNPSPGQQSKIRDLARLKKLVPIIKVNAKGF
                     PKFPKKFIKADHKRLPKALWKAKDPKQFKWLNDDLAKRNKNYDPDTQTFKNDPKKKKY
                     TWHHHQDKGRMQLVEFGAHSSTSHNGGRTTWSKGPRK"
BASE COUNT         1761 a         1906 c         2065 g         1722 t
ORIGIN      
        1 tttgcagacg ccggctttcg atttgcccgc gcagaccagt ttctttaacg cgtcaatcgt
       61 catttgctgc tggtcgagct gccgctgaaa ctgcgttaat tgctgctgct gccgctcgat
      121 tttcgcgtcg cggtctttaa gctccgcgta taagccctga atcgccgcaa acgcgatgcc
      181 gtccgggtca atcgtggaga tggtcttatc attttcgccg actttgaaaa cggacgaaaa
      241 atcctgcgcc atcacgccga tgtgcctgat tgaaggattt tgcgttttgt aattccacgt
      301 cgaaaccggc aagccgagaa cgccgcgcag gatttcgcgc gaatcgaccg cggcaaaatt
      361 ttccttcata ttgcggtccg agagattgtt ccaaccgccg ccgcccgccg ggagcgtgac
      421 gcccgccgat ccgccggagt tcgaggtgag cgtcgtcgag ccgtcgctcg ccatcgttaa
      481 tttgctgttg gcgagcgtca tcgtcgccgt cgtgctgttg aagatgaagc ccgtggtcag
      541 cgcggtgttg gtgtagaaga aagaaccgtt gatggcgcgg acgttgaacg agttcgtgac
      601 tgtcgggtga aactgcgtcg ctgtgtcggg cgaggtgctg ttgaacgtat aagtgagcgg
      661 gacggagcgg tcggagaaga cgaacgtgcc ggttcgcggc gccccggcaa gggtcgaggt
      721 ggacgcgccg taaccgagcg cgaccgagtt agcgccggtc gccgtgtgtc cttcgccgag
      781 agccaccgtg ccggtattag ccgcgaccga attcttgccg acgacgatgg cgttgtcgcc
      841 gagcgcgcgc acgttttcgc cgaacgcggt cgaatacagc ccgatgttgg cgtcgtccca
      901 ctgcgtgccg ttgatagagc cggctcggag cgctgctttc gcgccgaacc acatgaagcg
      961 cgtgcctgag ccttcaattg ggatgccgcc gcagcagccg ctgttgtagt cgctgacggc
     1021 gagaatcgag ccgtcgtttt cgacgcggaa gcgctccttg tcgaagtttt gagtgaagcc
     1081 gcctgaccag tacgttccgc tggcgctgag cgagaggagc gagtcctgac cggctgtgcc
     1141 gaggttgcgt gcgccgacga gcttgagatt gataaggtga tttgtggtgc tcgtcgtttg
     1201 ctggacggac ggcgcaagct cgacatcgcc gctgacctgg gcggtggtaa tgattccgct
     1261 tatagttaca gccgtcgtcg cgttcgtcgc gttagtggca tttgtggcat tcgcggcatt
     1321 cgtggcgttt atcgcattgg tggcgtttgt cgcattggtg gcgttcaaaa ccgttccggt
     1381 tattttcgag ccgtccagcg aaacaatctg cgcgctcgtc acgcacggat tgcaaaggct
     1441 cgagagcaaa tcggcggagc cggcgctgat gctgcgaacc gcataaggca cgctctcgat
     1501 tttctggcgc ggcgcgagcg tcgtcgcgcc gacctgaatc tccagccaga gcgactgacc
     1561 cgcggcgaat ggtccggtcg caaagtcgag cgacgtcgta aaaatgccgt tggtgaccgc
     1621 cacgctggag cttagctcgc tgccgacctg attgccgttt gcgagcgcgt cgtaaagccg
     1681 gaacttcatc gtataagttc cgttggtcgg ctgcgtgacg gtcgaatcgg taaagcgtcc
     1741 ctgataagtg aacgcggtgg tttgcgcggc aatcgagacg gcgcacgcaa tgaataatgc
     1801 aaacattaaa cttgcataac gaagcttttg tgatttattg ggcatagttt tccttcctga
     1861 aacggatgat attttcggtt aatgtagttt tgggtaaata cgggcgaacc ggcttgataa
     1921 acgagaagcg ggcttttgca agcctgttga ataataaccg aagccgcatt agcttgcaag
     1981 acattttgtc gcgcgcgcga tttaatctta aaatgaatac gctatgaaaa taccggaatc
     2041 agcgaaagaa gaatttgacc cgcgcgagca caccaaagaa ctcgccgccg aatttttgga
     2101 aaagggcgac gccaccggct ggtttgacgc cttgtataaa gaagccgaag gcaatcacga
     2161 gcatattccg tgggcagacc ttaagccgaa caaatttctg gcgcgattcg ccgaaaaaac
     2221 gaatttgcag ggcggcgggc gcagggctct ggtcgtcggc tgcgggctcg gcgacgacgc
     2281 gcggtttctg cacgatttgg gctttaaagt aactgctttc gacatttcgt cgacggcaat
     2341 cgaatgggcg aaaaagcttc acgcggaaac ttcgattgag tttttcgtcg ccgatttgtt
     2401 taacccgccg cagcattggc gcgagcgctt cgagttcgtg ctggaagttt acacgattca
     2461 gcctctgcct ttggaaatgc gcgaaaaggt gattgacgca atcgcgcagt ttgtcgccga
     2521 aaacgggaaa ttagtcgttg tcacgcgcgg tcgcgaagac gacgaacagc cgctcgagct
     2581 tccgtgggca ttatcgcgca aagacttgtc gcgctttgaa ttcaacgcgc ttaagcagac
     2641 gcattttgaa gaaatgccgg gcgatgaaga agagcctgtg agaagattcg ttgtcgaata
     2701 cctacggtaa cggaaaacgg aaagagaaag ataaaagaat cgtttctgca tttttcgctt
     2761 tcccgttttt atcaaatctc tttctccgcc aatttgctcc aatagcgcaa ttcgcggcgg
     2821 cgataatcgg gcgaggtttg cgccgattcg attgcctgtc ggaacatttc gcgggcgcgt
     2881 tcggtttcgc cctgcgcttt taaaactttg ccgagatggt ataagccttc ggcatcgacg
     2941 gagcgacgct cgacgtattt ttccagcgcc gcgcgcgcct cttcgagcat atttgccgcc
     3001 agataagtcg cgccgatttc gcgccagatt tcgctcagcg cgtgcttgtc gttttgctcg
     3061 acgaccgtgg agaaatgatt taaggcttgc tgcaaatcgc ctttttgccg cgcgattttg
     3121 ccgagctcgt aattggcgtc gggctcgctg gggtcaattt cgacggcgcg gttcagatgt
     3181 tccagagcct ttgtctcctg tcgtctttgt aaataaacca aagcgagctg aacgtgcgcg
     3241 tcggcgtcac gcgggttgac ggtcgcgttt tgcagaaagc gtttgaaatt ttgtttttgc
     3301 cgaaaggcgt cgctaaatcc gctgacctcg ccgcgcagat agccgccgaa atacatataa
     3361 gcgtaaagcg ccagaaacgg cgagaaaagc cagggcgaga cgaatcggaa aacgtacatt
     3421 cccgcgctga aggcgagcca cgaaagacaa accgtcacaa tcgccgcgcc gtaattgacg
     3481 ccgaaaaccg tgcgcagcgc gaaaatcatc agcgcgccga aaagaattcc gctcgccagc
     3541 cagaaagcaa aataaaccag cggcgaaacc gccggcgaat aaagcgcgat gcccgccacg
     3601 gcgaacggca gatgcgccgc cgcccaagcc atcagcgaac aggttgccag cgccgcgtaa
     3661 tcgcgccgca caacgacgcc gaaattaccg atattgccga aaatgcacag cagaagaatc
     3721 gtcgccggaa cgtaaaaaca ggaaatcgtc aaaaccggac gaaaaaatcc gctcggctcg
     3781 aaagaaaaga atcgaaagaa attatcgccg gcaaacggaa ctttttgccg cgcgtccatc
     3841 gccctgttat attcggcgac ggctctttca tactcggctt cggcggcggg cgcggattcg
     3901 ttcgtcgttg aataatccgg ctggtaaaac tcgtacagat tcgggacgtg atacgctttt
     3961 tccagtttgg aattgacggt cgaaaaaaaa gctagcgaga cgagaaatac cgcgacggcg
     4021 gcgaaaaacc agttgccgtt gtcgataatg tcgctcatcg ccgacagcgg gcgataaaaa
     4081 agcgcgagca gaagcttgat gttttccata tgtttgaacg agcgcgggct agggaattga
     4141 gaaacgcgcc ggaacccggt ttatagcgta ggttcaggcg ataaattttg tcaagatttt
     4201 tttctgcccg gtttttgagt aaaatcgaat tgcaactcga aaattttatc aaaggaattt
     4261 ctatcaaaat gtcggaagca accaaacagg atattaaaac acgcattgag acggattcga
     4321 tgggcgaaat cgcggtcgca tcggataaat actggggcgc gcagaccgag cgctcgctgc
     4381 atcatttcaa catcggtttc gatacgatgc cgcgcgagat gattcgcgcg ctcgggattt
     4441 tgaaaaaagc cgccgcgcag gttaatcacg agctgaaagc ggataaattg acgaaagaaa
     4501 agctcgattt aatcgtccgc gccgccgacg aggtcatcga aggcaggctc gacgcgcatt
     4561 ttcccctgcg cgtctggcag acgggaagcg gaacgcagac gaatatgaac gccaacgaag
     4621 tgatttccaa tcgcgccatc gaaatcgccg gcggcgagat gggcagcaaa aagccggttc
     4681 acccgaacga cgacgtgaat atgtcgcaat cgtcaaacga cactttcccg accgcgatgt
     4741 atattgccgc cgccgaacgc cttgacgccc tgattccgcg cgtgcgggaa gtttatgagg
     4801 cgattgacgc caaagcgaag gagtttgccg acgtggtgaa aatcggcaga acgcatttgc
     4861 aggacgcgac gcccttgacg gtcgggcagg aattcggcgg ctgggcgagc ctgatcgagc
     4921 gcgacatcga aagattgaaa ctggtaatga gcggcttgct ggatttggca atcggcggaa
     4981 ccgccgtcgg aaccggctta aactcgcatc ccgaattcgc cgaaaaagcc gctgccaaaa
     5041 tcgccgagct gacgggcttg ccttttaaag cgcatccgaa caaattcgcc gcgctgtcgg
     5101 cgcacgacga aatcgttttc gcctccggcg cgttgaaaac tctggcgggc agtctgatga
     5161 aaatcgcaaa cgacgtgcgc tggctcggct cgggtccgcg ctgcggcatc ggcgagctgg
     5221 ttttgccgga aaacgagccg ggaagctcga ttatgcccgg aaaagtcaat ccgacgcaga
     5281 gcgaggcgat gacgatggtt gccgcgcagg ttctgggaaa cgatgcggca atcggttttg
     5341 ccggctcgca gggaaatttc gaattaaacg tttttaagcc cgtgatgatt cataattttc
     5401 tgcattcggt gcgacttttg cacgacgcct gtcacggctt cgtcgaattc tgcattaacg
     5461 gaatccagtt aaatcgcgcg cagattgacg aatatctgaa cgattcgctg atgctggtga
     5521 cggctctgaa tcagcatatc ggttacgata acgccgcaaa gattgcaaaa aatgcgcata
     5581 agaaaggtct tacattgaag gaatcggcaa tcgagctggg acttttgacg ggcgagaaat
     5641 tcgacgagct cgtcaaaccc gaagacatga cgcatcctta aagatttttg atttttgatt
     5701 ttcggttgcg ggttgtggta aaacgccaca atccgctttt tttaacgcta tgaaaaaaat
     5761 atttctattt gttttattac tgagcgcgcc cgcttttgcg caggtaaagt ttgaaaaagg
     5821 caacgcttca aaaaactata atgcgcgaat cgaagtcgag cgctgcgccg aaggcacttg
     5881 cgacggcaag ctgaaaatcg agttatttaa gaaatccgcc gcgaaaccgt ttcaggttct
     5941 gcatctcgat caaacggaat ttgcggtcga agaagcaaag cagaccaacg ccaggcgcag
     6001 atatgattac cagtcgatga ttttttttga agactataat ttcgacggca tcgaagattt
     6061 atcaatcaga gacggcaaca acggagctta caacggacct tcgtatcaga tttacctgtt
     6121 ctcgccgccg cgcggaaaat tcgtccacag cccggctttg actcgtctgg cgcagagcga
     6181 atatctggga atgttcgagg tcgataagaa aaagcgagtc atccgcgtcg cttccaaagg
     6241 cggctgctgt atgcaccgaa aggaagaatt tcgcgtggtg aataatcgcc tcgtcaaagt
     6301 tttcgagatt tacgaggaag tcgagttttg gaaagaaaac gtcaaccgcg tccgaatcga
     6361 gacgagccgg ttaataaagg gacgctggca aacgcgcgtc agatacgaaa agataaaaga
     6421 ttaaaatttt aaaagcccga gcctgttttg ctcaaacgct cgcgggctcg ggcttgaaca
     6481 ttttcccgcc ccgtttcttt cccgccgggc ttttcatcgg ctacgaatct ttgccgcctt
     6541 cttcgtcatc ggtcagcatc gacaaaaact gtctgaaatt gtcgacgacc ggaatcgtgt
     6601 aaggcttcat ttctccggac aaatcgtggt cccacaaaac gattttcgcc tgcccgccgc
     6661 cttcgtaatc gaaacaaaga aaatcgccgg gataaattat cgcaaacgga acgagatatt
     6721 cgtttaagcg gtcgctgatt tgcgaccaga cgacggcgac atcgtaatag ccgaattcct
     6781 cgttgtcctc gtaatcggga acaaagctca gaaaacgctc gacgaccttg acgttgtcgt
     6841 ccagcggaaa aaactgtttt ctcggcacgc cgccgttgtg tttttcgaga aacgccagat
     6901 aatcaggctc gaatttcagc ctcagcatat cttcggtttc cttgacgtat cggtcatcga
     6961 attctccggc ttcggttccc ggcagaaact ccaaatcgaa atcttcgctc atctgtttat
     7021 ccttgctgaa attttatttt ctcggtcctt tcgaccaggt tgttcgcccg ccgttatgag
     7081 aggtcgaact gtgcgcgccg aactccacca actgcattct acctttgtcc tgatgatgat
     7141 gccacgtata ttttttcttt ttcgggtcgt ttttaaaagt ctgcgtatcc gggtcgtaat
     7201 tcttgtttct tttcgccaga tcatcattca accatttgaa ctgtttaggg tctttcgcct
     7261 tccagagcgc tttcggcaat ctcttatgat ccgctttgat aaactttttc ggaaacttcg
     7321 gaaagccttt cgcattaact ttaataatgg gaaccaattt tttcagccga gccaaatcgc
     7381 ggattttcga ctgctgtccg ggcgacggat tgccgagcat tttccacgac cacggcttgc
     7441 tggcattaat gatt
//
LOCUS       DASWNE010000354         7737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_81549, whole genome shotgun sequence.
ACCESSION   DASWNE010000354 DASWNE010000000
VERSION     DASWNE010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7737
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81549"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VGB68_07175"
     CDS             <1..732
                     /locus_tag="VGB68_07175"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY0049049.1"
                     /translation="IIGAEQLNGLIDDQKDFSAAFQTASRFKINAGNLEGYTTRYDDI
                     IANAVEWCPGLSPAILKSLLAQESRFNPTVINQYGYAGIAQFGRPAAREVGLNVGIAG
                     SSSDERLDPSKAIPGAARLLNIKAKRLGEIAFSRYGQPDGIEFWKFVTAAYNGGEGTV
                     ALAMGHAYRNGLAQARSQGLVGVEAVSFARRYASKWENLKIGGADSPLALAAARYFPN
                     LAVQKFHEIGNYPTQIVARVAGKFK"
     gene            745..2556
                     /locus_tag="VGB68_07180"
     CDS             745..2556
                     /locus_tag="VGB68_07180"
                     /inference="COORDINATES: protein motif:HMM:NF025792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HEY0049050.1"
                     /translation="MGDNTISNDSGSVSSIEQRRLVEAADANTTLEPPADEKKISQKS
                     PPVPPHVSVRSLEGESPRELHRRILLEAASQAGLDGTPQQKKFVEMFLGGLKYREGDP
                     SPQDDATVKPAEIKNQSFNYRLEPEEIIALRQLQKDYAAQGGQFREGKVYDYSADGRT
                     LTPSEKRNGRRGITAEQYEQWRLQDLMRKAQILPSSFGTPSRLRDLKHITLDAPRDGN
                     PVTEDRALRLYIQSRFGAANGGNLWGDKLNEIFELAKSRGVRIENLDSENGKIGFDLS
                     VENLLKLQHAYIGVQEKFNADMEIVNKTVDNLAHNRFAKGVLEGAWESLKANWNAITN
                     PIETIKSVYEAAKTLGNLGLELAQMQPEERAELFADLAKAGIKGLAEMPLRDAAEYLG
                     KAVGTLAVEIALGKGAGLVFRALSLTKFGASLANRTRILSGEIKQTIGKIPVPTNASV
                     KIVVDTTGNRMIFPDFELTKLEDLINPLESRAQQALSGTKKAVNLPAWEKLTFKLDDD
                     GSIHLLSGHKVGGNRLKSSRNAGGRKDVFPEWMSDKQILKAVEQAYKSGKKVGTQVDF
                     ATGDKIIELVGYSDDGLKIRIYVNVTKKQLDTAFPQK"
     gene            2566..3048
                     /locus_tag="VGB68_07185"
     CDS             2566..3048
                     /locus_tag="VGB68_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049051.1"
                     /translation="MQEFIIHLPIASYQRKNEKFDNFGEIYLQLGDYFFPSENWTDFG
                     ANIVFWWLQEITKIYSNESDTARCKFMDGNYRFDLQITDRPEIWQIYFIKERADDDEI
                     ELQTEVNSEQLSREILKAVTKIEDQYREMKNFEAVNRIEEIKEIFILSAKPLNAAFPH
                     "
     gene            complement(3161..4018)
                     /gene="sctT"
                     /locus_tag="VGB68_07190"
     CDS             complement(3161..4018)
                     /gene="sctT"
                     /locus_tag="VGB68_07190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HEY0049052.1"
                     /translation="MQEFLQLLELVLKTFEVGQSPQAFLVLFGLSFARFISFIVIVPF
                     FGGQVVPSQVKVATATALVIITYPSLLAELPTDGSPLGFGAIGFVGLLAKEVFVGFTL
                     GFVASLVFEAVAVAGRIADFQRGSTMAELFSPQLQERVSELGQFQLQLAIVIFLTTGA
                     HRFFISALVRSFEFIPATRFPQIEAGWTPAAEFMTVVTGSVLSIGVQLAVPVVVTLLL
                     TDLFFGLINRVAPQINVFFLSMPVKMWVGIFVVLIALPYMVARFVDYFDMSYEAFEFV
                     IRYFGRMYQ"
     gene            complement(4097..4366)
                     /gene="fliQ"
                     /locus_tag="VGB68_07195"
     CDS             complement(4097..4366)
                     /gene="fliQ"
                     /locus_tag="VGB68_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HEY0049053.1"
                     /translation="MDIYTQLAGEALWLILVLSLLPLLASLIVGLVVSLFQALTQVQE
                     QTLTFVPKIIATILVIMITAPWMMEVLQEFSTRSFDLILKVSVRR"
     gene            complement(4392..5171)
                     /gene="sctR"
                     /locus_tag="VGB68_07200"
     CDS             complement(4392..5171)
                     /gene="sctR"
                     /locus_tag="VGB68_07200"
                     /inference="COORDINATES: protein motif:HMM:NF009438.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctR"
                     /protein_id="HEY0049054.1"
                     /translation="MKQRLWSLFFGAFLFIGLLPLGALAQNAPAALPNQPAADTANPI
                     VLIIVIGALALAPFGLIMLTSFVKIAVVLSILRNALGTQQVPPNQVITGIALILTVFI
                     MAPVVEKMHAEAGVVQNTEAIFSEVSVKTLFEASQRGKEPLRQFLLRYAEERHRVMFF
                     QLAQQMAQRNGNNPDEIKPEDFRVIIPSFVTSQLTEAFQIGFFLFVPFLIIDMVVANI
                     LQAMGMFMLSPTIISLPFKLLLFVLINGWELLIKNLVLGYV"
     gene            complement(5171..5560)
                     /locus_tag="VGB68_07205"
     CDS             complement(5171..5560)
                     /locus_tag="VGB68_07205"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HEY0049055.1"
                     /translation="MGNSLNFLWMLVQTVMALAFVCGLAYLIFRVIMPRLSVGFSSNN
                     MVRVIDRIGLEARKNLYVIEVAGRFLLIAASESGVQLIAELDAEAAAEAERELAQMRQ
                     APRGNPVLGKSFADKLNDVLNRKQGGK"
     gene            complement(5632..7089)
                     /locus_tag="VGB68_07210"
     CDS             complement(5632..7089)
                     /locus_tag="VGB68_07210"
                     /inference="COORDINATES: protein motif:HMM:NF013236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HEY0049056.1"
                     /translation="MKTSERTIAGESEAEENFAPGDLSLDPFAVDIFGKENAVETEKT
                     SPLPVEAAQAIGLRTNQAKRAAWFAALPRVTRREAEFSNSFLELPDNISASAVKKIEE
                     TIAYYTLRPTHDVKCSLISVAEANLAEAVDEASKAPHVFLTLGCQPDNSRALIAINGD
                     FAASIINSVLIDDGTTVFAIRELSAIERTIIEFLGINILGEVNNLLGQNLFCLQSVAT
                     VASEPFGAGERGAEIVVSLNFKTFGGALSLFASRRFLTTLSAASRGGLSEKASNKTAL
                     QTLERFVPKLNLNALIGTTRLSGADLNYLEPDDIIIIDQPEIDWQSAAFQGGLQVYLG
                     SGRNFRLIGNASDAGNNAELKLKIEEISVGEERSASPTEIRMQMNETENNLAETETGE
                     RATETGEQFSGENAEENPPALENVLVTLRVELGGGKISLREIQNLRAGQIISLGARPT
                     DPVRIVTDAGEQPVATGELIEIEGQLGVRLTKIFI"
     gene            complement(7172..>7737)
                     /locus_tag="VGB68_07215"
     CDS             complement(7172..>7737)
                     /locus_tag="VGB68_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049057.1"
                     /translation="SQTEFPVGQPGAAQPSVDTPNLPPGQKILPDAVTGRDETQLEAA
                     RQLSTLGGTSATDRLLGLDLRPTMTGALSAPPGNSEFLRHLSPQVRRTIMRKMLTKQR
                     ERMRRLARFLRERHDESAGGETDSNGDEDRESFLEVISEPRELDRAQITRATGELDKA
                     ARMLDILDEMLTMQDYTISQIGTFSQG"
BASE COUNT         1967 a         2066 c         2052 g         1652 t
ORIGIN      
        1 atcatcggcg cggaacaatt aaacggcttg attgacgacc agaaggattt ttccgcggcg
       61 tttcaaaccg ccagccgttt taaaatcaac gcgggaaatc tcgaaggcta cacgacgcgc
      121 tacgacgata ttatcgccaa cgcggtcgaa tggtgcccgg ggctttcgcc cgctattttg
      181 aaaagtttac tggcgcagga atcgcggttt aacccgacgg tgattaatca atacggttac
      241 gcgggaattg cgcagttcgg cagacccgcc gcgcgtgagg tgggcttaaa cgtcggaatc
      301 gccggttcga gcagcgatga gcgcctcgac ccttcaaaag cgattcccgg cgcggcgcgt
      361 ctgttgaaca tcaaagccaa gcgcctgggc gaaatcgctt tttcccggta tggtcagccc
      421 gacggcatcg agttctggaa attcgtgacg gccgcttata acggcggcga aggaacggtc
      481 gcgctggcga tgggacacgc ttaccgcaac ggcttggcgc aggctcgctc gcagggtttg
      541 gtcggcgtcg aagccgtcag cttcgcacgt cgttacgcgt cgaaatggga aaatttgaaa
      601 atcggcggcg cggattcgcc gctcgcgctt gccgccgcgc gttattttcc gaatctcgcc
      661 gtgcagaaat ttcacgaaat cggaaattac ccgacgcaaa tcgtcgcgcg cgtcgcggga
      721 aaatttaagt aagtcgaaag ctttatgggc gacaacacca tttccaacga ttcggggtca
      781 gtctcttcca tcgaacaacg gcggctcgtc gaagccgccg acgcaaatac cacgctcgag
      841 ccgcctgccg acgaaaaaaa aatatcgcaa aaatcgccgc ccgttccgcc acatgtttca
      901 gtgaggagcc ttgaagggga atcgccgcgc gagctgcatc gccggatttt gctcgaagcc
      961 gcgagccaag ccggactgga cggaacgccg cagcagaaaa aatttgtcga gatgtttctc
     1021 ggcggtctta aatatcgcga gggcgacccg tcgccgcagg acgacgcgac cgtaaaaccc
     1081 gccgagataa agaatcaatc ttttaattac cgcctggagc cggaagaaat cattgctctg
     1141 cgtcaactgc agaaggacta cgccgcgcag ggcgggcagt ttcgcgaggg aaaagtttac
     1201 gattattcgg cggacggcag aacgctgacg ccgagcgaaa aacgcaacgg caggcgcggc
     1261 atcaccgccg agcaatacga gcagtggaga ttacaggatt tgatgcgcaa ggcgcagatt
     1321 ctgccttcca gcttcggcac gccgagccgg ctgcgtgatt taaagcacat tacgctcgat
     1381 gcgccgcgtg acggcaatcc ggttacggaa gaccgcgcct tgcgccttta cattcaatcg
     1441 cgtttcggcg cggcaaacgg cggaaacctg tggggcgata agctgaacga aattttcgag
     1501 ctggcgaaat cgcgcggcgt gagaatcgaa aatctggatt cggagaacgg caaaatcgga
     1561 ttcgacctgt cggtcgaaaa tcttttaaaa ctccagcacg catatatcgg cgttcaggaa
     1621 aaatttaacg ccgatatgga aatcgttaac aaaacggtgg acaatctggc gcacaatcgt
     1681 ttcgccaaag gcgttttaga aggcgcctgg gaatcgctga aagcaaactg gaacgcgata
     1741 accaatccga tcgaaacgat taaaagcgtt tatgaagcgg cgaaaacgct cggcaatctc
     1801 ggtttggagc tggcgcagat gcagcccgaa gaacgcgccg aattgtttgc cgatttagcc
     1861 aaagcaggca ttaaaggctt ggcggaaatg ccgctccgcg acgcggcgga gtatttagga
     1921 aaagccgtcg gcacgctggc ggtggaaatt gctttaggca aaggcgcggg cttggtgttc
     1981 agggctttaa gcctgacaaa gttcggcgcg tcgctggcga acagaaccag aatcctaagc
     2041 ggcgaaatca agcagaccat cggcaagatt ccggttccga ccaatgcctc cgtcaaaata
     2101 gtcgtcgata cgacgggcaa caggatgatt ttcccggact tcgagctgac gaaactcgaa
     2161 gaccttatca acccgctgga atcgagggcg cagcaagcct tgtcgggcac taagaaagca
     2221 gtcaatctgc ccgcctggga aaaattgacg tttaagctgg acgacgacgg ctcgatacat
     2281 cttctttccg ggcacaaggt cggcggaaat cgcctgaaat caagccggaa cgccggcggg
     2341 cgaaaagatg ttttcccgga atggatgtcg gacaagcaaa ttctgaaagc cgtcgaacag
     2401 gcttataaat cgggtaaaaa agtcggcacc caggtagatt tcgcaactgg agataaaatt
     2461 atcgagttag tcggctattc ggatgacggt ctaaaaatca gaatttatgt taatgtaact
     2521 aaaaaacaat tggacacggc atttccgcaa aaataaacaa agcgtatgca agaatttatt
     2581 attcatttgc ccattgcgtc atatcaaagg aaaaatgaaa aattcgataa tttcggcgag
     2641 atttatttgc agctcggcga ttattttttt ccgagtgaaa actggacgga tttcggcgcg
     2701 aatatagtat tttggtggct gcaggaaatt actaaaattt actcgaacga gtctgataca
     2761 gcccgctgca aatttatgga cggaaattat cgattcgacc tgcaaataac cgatagacct
     2821 gaaatctggc agatatattt tattaaggaa agagccgatg atgacgagat agagctccag
     2881 accgaagtaa attccgaaca gctttcccgg gaaattttaa aagccgtcac gaaaatcgaa
     2941 gaccagtacc gggaaatgaa aaattttgaa gccgtcaacc ggattgaaga aataaaggaa
     3001 atatttattt tatccgccaa gccgctgaat gccgcctttc cgcattaaat aacgaataag
     3061 tccgaagttt atcaaacctg aaaacaccgc aaacaatcaa tcaccgattt tccggttccg
     3121 aagttattta ttattttcct ttatttcgcc aaagctcaaa tcattgatac atccgaccga
     3181 aataacgaat cacaaactcg aacgcttcat acgacatatc gaaataatcg acaaaacgcg
     3241 cgaccatata aggcagcgcg ataaggacga cgaaaattcc gacccacatt ttcaccggca
     3301 tactcaggaa aaaaacgttg atttgcggcg cgacgcgatt gataagcccg aaaaacaaat
     3361 cggttaaaag cagagtgacg acgaccggaa cggcaagctg gacgccgatg ctcaaaacgc
     3421 tgcccgtcac gaccgtcata aattccgccg ccggcgtcca gcccgcttcg atttgcggaa
     3481 agcgcgtcgc cggaataaac tcgaagctcc tgaccagcgc gctgataaaa aaacgatgcg
     3541 cgcccgtcgt cagaaaaatg acaatcgcca gctgcagttg aaattgcccg agctcggaaa
     3601 ctctttcctg caactgcggc gagaaaagct cagccatcgt cgagccgcgc tggaaatcgg
     3661 cgattcgtcc ggcgacggcg acggcttcaa aaaccagcga cgcgacaaat ccgagcgtga
     3721 aaccgacaaa aacttctttc gccagcaagc cgacaaagcc gattgcgccg aatccgagcg
     3781 ggcttccgtc ggtcggcagt tcggcgagca gcgaaggata cgtgattata accagagcgg
     3841 ttgcggtagc gaccttgacc tgcgacggga cgacctgacc gccgaaaaaa ggaacgataa
     3901 cgataaacga gatgaaacgc gcgaacgaca agccgaacaa aaccagaaag gcttgcgggc
     3961 tttgtccgac ctcgaaggtt ttcagaacga gttctaaaag ctgtaaaaat tcctgcacaa
     4021 cttaaaagat tcaggattca gaattcaaaa ttcaggattg gcgttttcaa tcttgaatcc
     4081 tgaatcctga attcattcag cgtcggacgc tgactttcaa aatcaaatca aacgagcgcg
     4141 tcgaaaattc ctgcaaaact tccatcatcc acggcgcggt aatcattatg accagaatcg
     4201 tcgcgatgat tttcggcacg aaagttaaag tctgctcctg cacctgcgtc agagcctgaa
     4261 aaagactgac gaccagcccg acaatcaggc tcgccagcag aggcaaaagc gacaatacca
     4321 gaatcagcca taaagcttcg cctgccagtt gcgtgtaaat gtccattttc cccggtctcc
     4381 tttcgtcctc gtcagacgta gcccagcaca agatttttaa tcagcagctc ccatccgttt
     4441 atcagcacga acagaagcag cttgaacggc agcgaaataa tcgtcggcga cagcataaac
     4501 attcccatcg cctgcaaaat attggcgacg accatatcga taatcaaaaa cgggacgaag
     4561 agaaaaaagc cgatttgaaa cgcctcggtc agttggctgg tgacaaaaga cggaataatg
     4621 acgcgaaaat cttccggctt gatttcgtcc ggattgttgc cgttgcgctg cgccatctgc
     4681 tgcgcgagct gaaaaaacat cacgcggtgt cgctcttcgg catagcgcag aagaaactgg
     4741 cgcagaggct ctttgccgcg ctgcgaggct tcaaacagag ttttgacgga aacctcgctg
     4801 aaaatcgcct cggtattttg aaccactccg gcttcggcgt gcattttttc gacgaccgga
     4861 gccattataa aaacggtcag aatcagcgca attcccgtaa taacctgatt cggcggaacc
     4921 tgctgcgtgc cgagcgcgtt gcgcaggatg gaaagcacga ccgcgatttt gacgaacgag
     4981 gtcaacatta tcaaaccgaa cggcgcgagc gccagcgcgc cgatgacgat gattaaaaca
     5041 atcgggttgg cggtgtcggc ggcgggctga ttcggcagag cggcgggcgc gttttgcgcg
     5101 agcgcgccga gcggaagcag accgataaac agaaacgcgc cgaaaaaaag gctccacaga
     5161 cgctgtttca tcattttcct ccttgcttgc gattcagaac gtcgttcaat ttatcggcga
     5221 acgattttcc gagaacggga tttccgcgcg gcgcttgccg catctgcgcc agctcgcgtt
     5281 cggcttcggc ggcggcttcg gcgtcgagct cggcgataag ctggacgccg ctttcgctgg
     5341 cggcgatcaa cagaaagcgc ccggcgacct cgatgacata cagatttttc cgcgcttcca
     5401 acccgatgcg gtcgatgacg cgcaccatat tgtttgacga aaagccgacc gataaacgcg
     5461 gcataatgac tcggaaaatc aggtaagcca gcccgcagac gaacgccagc gccatcaccg
     5521 tctgcaccag catccacaaa aaatttaaag aatttcccat attttgccgt ttaaagcggc
     5581 gaattaaaca cagattttcg ctgaatgcgc ggctttaaac gcgccgcgcg ctcagataaa
     5641 aattttcgtc aggcgcacgc ccagctgacc ttcgatttcg attaattcgc cggtcgccac
     5701 gggctgttcg cccgcgtccg tgacgattct aaccgggtcg gtcgggcgcg cgccgagcga
     5761 gatgatctgt ccggcgcgca ggttctgtat ctcgcgcagg ctgattttgc cgccgcccag
     5821 ctcgacgcgc agagtcacca gaacattttc cagcgccggc ggattttcct ccgcgttttc
     5881 gcccgaaaat tgttcgcccg tttccgtcgc ccgttcgccc gtctcggttt cagccaggtt
     5941 attttccgtc tcgttcattt gcattctaat ttcggtgggc gacgcgcttc gctcttcgcc
     6001 gaccgaaatt tcctcgattt ttaattttaa ttccgcattg tttcccgcgt cgctcgcgtt
     6061 tccgattaaa cgaaaatttc tgccgctgcc gagataaacc tgcaagccgc cctgaaaagc
     6121 cgcgctctgc cagtcaatct ccggctggtc gatgatgatt atgtcgtccg gttcgagata
     6181 atttaaatcc gcgccgctca atctggtcgt gccgattaaa gcgttcagat ttaatttcgg
     6241 cacgaatcgt tcgagcgtct gcagggcggt tttgtttgaa gctttttccg ataagccgcc
     6301 gcgcgaagcc gcgctcaaag tcgtcagaaa ccggcgcgag gcaaaaaggc tcagcgcgcc
     6361 gccgaaagtt ttgaaattta agctgacgac gatttccgcg ccgcgctcgc ccgcgccgaa
     6421 cggctcgctc gccacggtcg ccaccgattg caggcagaac aaattttgac cgagcagatt
     6481 attgacctcg cccaaaatgt taatgccgag aaattcgata atcgtgcgct cgattgcaga
     6541 taattcgcga atggcgaaaa cggtcgtgcc gtcgtcaatc aaaaccgaat tgataatcga
     6601 agcggcaaaa tcgccgttga tggctatcag cgcgcgcgag ttatcgggct gacagccgag
     6661 cgtcagaaaa acgtgcggcg cttttgaagc ctcgtcgacg gcttccgcca gattcgcttc
     6721 ggcgacggaa atcagcgagc atttaacgtc gtgcgtcggg cgcaaagtgt aataagcgat
     6781 tgtctcttcg atttttttaa cggctgatgc ggagatatta tcaggcagtt cgagaaacga
     6841 gttggagaat tcggcttcgc ggcgcgtcac gcgcggcagc gcggcaaacc acgccgcgcg
     6901 ttttgcctga tttgtccgca aaccaatcgc ctgtgcggct tcgaccggaa ggggcgacgt
     6961 tttttcggtt tcgacggcgt tttccttacc gaaaatatcg acggcaaaag ggtcgagtga
     7021 caaatcgccc ggcgcgaaat tttcttccgc ctcgctttcg cctgcaattg ttctctccga
     7081 ggttttcatt ttttaaattc aggattcaga attcagaatt caggattgcc ggtttcaaat
     7141 cttgaatcct gaatcttaaa tcctgaatca tttacccttg cgaaaaagtt ccgatttggc
     7201 tgatggtgta atcctgcatc gtcagcattt cgtccagaat gtcgagcatc cgcgccgctt
     7261 tatcaagctc gcccgtggcg cgcgtgattt gcgcgcggtc taattcgcgc ggctcggaaa
     7321 tgacttccag aaagctttcg cggtcttcgt cgccgttgct atcggtttcg ccgccagccg
     7381 attcgtcgtg ccgctcgcgc agaaatcgcg ccagtcgccg cattcgctcg cgctgtttgg
     7441 tcagcatttt gcgcataatc gtgcggcgca cctgcggcga caggtgacgc agaaattcgc
     7501 tgtttcccgg cggcgcggaa agcgcgccgg tcatcgtcgg gcgcaaatcg agcccgagca
     7561 gacggtcggt tgccgacgtg ccgccgagcg tgctcaattg ccgggcggct tcgagctgcg
     7621 tttcgtcgcg tccggtgacg gcgtcgggca aaattttttg tcccggcggc agattcggcg
     7681 tatcgaccga aggctgcgcc gcgcctggct gcccaaccgg aaattccgtc tggctgg
//
LOCUS       DASWNE010000355         2602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_681620, whole genome shotgun sequence.
ACCESSION   DASWNE010000355 DASWNE010000000
VERSION     DASWNE010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681620"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            394..1329
                     /locus_tag="VGB68_07220"
     CDS             394..1329
                     /locus_tag="VGB68_07220"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY0049058.1"
                     /translation="MKNLFFTFILLFGTINIFAQTKNPPPKSVSAGIVNGKATSLPKP
                     EYPAAAKAVRAGGGVNVEVTIDENGDVISARAVSGHPLLQQAAAAAARQAKFKPTLLA
                     GQPVKVTGVIVYNFVAPPSSPASSAGNKSEESEELVFYTGLSMFLTALKDIEPDEEAN
                     SILREMASGFPAEFAAEREQLNRLSNAKRSEQPRIIDEFTASFGRRLSGTELWLFDFG
                     KHWGSALGQAYKLSGGQMPGDKVKFIDSINKMTALLNSAPKDIPAEILEKIRKIALVN
                     DENDMVSPEFINDFFKASFEFIEFISPEEKKDGTN"
     gene            1316..1732
                     /locus_tag="VGB68_07225"
     CDS             1316..1732
                     /locus_tag="VGB68_07225"
                     /inference="COORDINATES: protein motif:HMM:NF022281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HEY0049059.1"
                     /translation="MEPTSSPTERILATAVLLGGGAAAFAVGWFNPVTAGFFPQCPLY
                     QTTGLACPGCGLTRGFHALFHGDVLTALDYNALLPFYVLFLAYLAASLASVVVRGRGF
                     SFKLFQPKVAYSFLVISLVFAVVRNLPLYPFSILYP"
     gene            complement(1800..>2602)
                     /gene="ispG"
                     /locus_tag="VGB68_07230"
     CDS             complement(1800..>2602)
                     /gene="ispG"
                     /locus_tag="VGB68_07230"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF016438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HEY0049060.1"
                     /translation="NIGSIERVREVVRATEAQGVPIRIGVNGGSLEKDLLAKYGTATP
                     EAMVESGMRHVRILEDLGFTDIIISLKASDVNRMVAAYRLMAEKVDYPLHLGVTEAGT
                     PFGGTIKSAMGLGILLHEGIGDTIRVSLAAEPHEEVRVGWEILKSLELRKRGVTVVAC
                     PTCGRLDVDNFVEIVTEVERRLAHVEEPLHLSIMGCAVNGPGEAHDSQLGVTFGRNVG
                     MIFKNGVPMRRVAGADIVEEFVKEVEILRQEGDKAKSLLEEKPLVQIS"
BASE COUNT          586 a          687 c          666 g          663 t
ORIGIN      
        1 ctgacggaaa tcgaaaccct gcgcgaaaac tttaccgtta atctcaaccg caaaatcgcg
       61 accgacgcgc tttttatgca gatggcgcat aattaaataa aaaaatttag ccgccgcaga
      121 atactgagag ataaagagaa aaccccgcag acagatacga gataaacgca gattattaac
      181 aggaagatgt aagctttgcg gcttgacgga acaggataaa tccttttctt atccgtcttt
      241 atttttatct ctcgcttacc agcgaaaatc tgcggtttca ttccctcttt tctccgcgtt
      301 ccgcggcggc tgattttccc gtcgccaggt aaatgcattt ggttttaacc cggtttgttt
      361 tataatcgga gtctgcccgg aaaaggagtt tttatgaaaa atttgttctt cacgtttatt
      421 ttgcttttcg gcacgataaa tatttttgcc cagacgaaaa atccgccgcc gaaatctgtt
      481 tcggcgggca tcgtcaacgg aaaggcgacc agcctgccga agcccgaata tccggcggcg
      541 gcaaaagcgg ttcgcgccgg cggcggcgtt aatgtggagg ttacaatcga cgaaaacggc
      601 gacgttattt ccgcccgagc cgtttcgggt catccgcttc tgcaacaggc ggcggcggcg
      661 gcggcgcggc aggcaaaatt taaaccgacg ctgcttgccg ggcaacccgt taaagtgacg
      721 ggcgtaatcg tttacaactt tgtcgccccg ccttcttctc cggcgtcgtc agccgggaat
      781 aaatcggaag aatcggaaga actggttttc tacaccgggt tgagtatgtt tctgacggct
      841 ttgaaggaca tcgagccgga cgaggaagca aacagtattc tgcgggaaat ggcgagcggt
      901 ttccccgccg agtttgccgc cgaaagggaa cagcttaaca ggctgtcgaa cgccaaacga
      961 agcgaacagc ctcgaattat cgacgagttt accgcttctt tcggacgtcg tttgagcggc
     1021 accgaactgt ggctgttcga tttcggaaaa cattggggaa gcgctctggg gcaggcttat
     1081 aaactttccg gcgggcaaat gcccggcgac aaggtcaaat tcatcgacag cataaacaaa
     1141 atgacggcgc tgctgaattc cgcgccgaaa gatattccgg cggaaattct ggaaaaaatc
     1201 agaaaaatag ctttggttaa tgatgaaaac gatatggttt cgccggaatt cattaacgat
     1261 ttcttcaagg caagctttga gtttattgag tttatctctc ccgaagaaaa gaaagatgga
     1321 accaactagc tcaccgaccg aacgaatttt agcgacggca gttttgctcg gcggcggcgc
     1381 ggcggcattc gcagtcggct ggtttaatcc ggtgacggca ggattttttc cgcaatgccc
     1441 gctttatcag acgaccggct tggcgtgtcc gggctgcggg ttgacgcgcg gttttcacgc
     1501 gctttttcac ggcgacgttt taacggcgct cgattataat gcgctgctgc cgttttacgt
     1561 tttatttttg gcttatctgg cggcttcgct cgcttccgtc gtcgtgcgcg ggcgcggttt
     1621 ttcgtttaag ctttttcagc cgaaagtcgc ttacagcttt ctggttattt ctctggtctt
     1681 tgcggtcgtg agaaatctgc cgctttatcc tttctcgatt ttatacccgt aatttggaaa
     1741 gtgaaaaagt gaaaaagtaa tggttgtttc tcgaccgcaa actttttcac tttttcactt
     1801 taagaaatct gcacgagcgg tttttcttcc agcagggatt ttgctttatc gccttcctgc
     1861 cgcaaaatct cgacttcttt gacaaactcc tcgacaatat ccgcgccggc gactcggcgc
     1921 atcgggacgc cgtttttgaa aatcattccg acgtttctgc cgaacgtgac gcccagctgc
     1981 gaatcgtgcg cctcgcccgg accgttgacc gcgcagccca taatcgaaag atgaagcggt
     2041 tcttcgacgt gtgccaggcg gcgctcgact tcggtgacga tttcgacaaa attatcgaca
     2101 tcgagccttc cgcacgtcgg gcaggcgacg accgtgacgc cgcgttttct cagttccaga
     2161 cttttcaaaa tttcccagcc gacacgcact tcctcgtgcg gttccgccgc cagagaaacg
     2221 cgaatcgtgt cgccgatgcc ttcgtgcaga aggatgccca agcccatcgc cgattttatc
     2281 gtgccgccga aaggcgttcc cgcctcggtg acgccgagat gcaatggata atcgaccttt
     2341 tccgccatca ggcgataggc ggcaaccatc cggttgacgt ccgacgcttt taagctgatg
     2401 ataatgtccg taaaacccaa atcttcgaga atccgcacgt ggcgcattcc cgattcgacc
     2461 atcgcttcgg gcgtcgccgt gccgtatttt gccaaaaggt ctttttccag cgagccgccg
     2521 ttaacgccga ttctgattgg gacgccctgc gcttccgtcg cgcggacgac ttccctgacc
     2581 ctttcaatcg agccgatgtt tc
//
LOCUS       DASWNE010000356         1996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_436391, whole genome shotgun sequence.
ACCESSION   DASWNE010000356 DASWNE010000000
VERSION     DASWNE010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436391"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1933)
                     /locus_tag="VGB68_07235"
     CDS             complement(<1..1933)
                     /locus_tag="VGB68_07235"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049061.1"
                     /translation="MLFLYSSEGSTSGQGSRGAVTNREYFVKLSEFIVKLVGGQSSGE
                     GAAYRVDLRLRPHGRVGALAISVRDAVNYYRTSAQAWERQTMIRSRSAAGDAEIFKSF
                     FASIETSVFSKDETVENALRNVRLSKEKINLEKLSGRGFNVKLGKGGIREIEFIAQAL
                     QIAYGARDAWLRAPHTLISLSRLADRKLLTENDLTELADAYDFLRRLEHRLQMEHGLQ
                     THLVPEDAEKRRVIAKRMNCPSPESFDAELNKHAESVNRIFARLFEETGDAENVTEEG
                     LATARGARQNANRFGLNSTLNSFDSPVRVRALAGQTPDLPLQIVSALEKSDSPFDLTE
                     KKLAALKKFCRISPSFTEMIAANPKLIESLPLETEDFEAVDYAENLGAAVRRQANFHD
                     ELAALRIEWTKSFLQIAAFDVFEKIDLAESKRLQTELAEAGLSVAFEITGREVRRRFD
                     FSGELRLAVLALGKLGGRGMDYGSDLDLVLIYDDKNSETVSTVSADARLPDSPALFYA
                     RAAEIFVTTLSSFMREGHLYRVDLRLRPDGKNGVTVLGENAFYSYLETRAAIWEWLAY
                     VKLRAVGGKADLASEIEKKARRIIHQNATEADRNDLKTETAHVRERLEKEKAPSAKDF
                     NIKYGAGGMLDVYFAMRFLQ"
BASE COUNT          446 a          576 c          523 g          451 t
ORIGIN      
        1 attgcagaaa gcgcatcgca aaataaacgt cgagcattcc gcccgcgccg tattttatat
       61 taaagtcttt cgcgcttggc gctttttctt tttccagtct ttcgcggacg tgcgccgttt
      121 ccgttttcaa atcgttgcgg tcggcttcgg tcgcgttttg atgaataatt cggcgcgcct
      181 ttttttcgat ttccgaagcc aaatccgcct tgccgccgac cgctcgcagc tttacgtaag
      241 cgagccattc ccagattgcg gcgcgcgttt ccagataact gtaaaacgcg ttttcgccca
      301 aaaccgtgac gccgttttta ccgtcgggac gcagacgcaa atcgacgcgg tagagatgac
      361 cttcgcgcat aaaactcgaa agcgtcgtca cgaaaatctc cgccgcccgc gcgtagaaga
      421 gagccgggga gtcgggcagc cgggcatctg cggagacggt cgaaaccgtt tcggaatttt
      481 tatcgtcgta aatcaagacc aaatccaaat ccgagccgta atccataccg cgcccgccca
      541 gctttccgag cgcgagaacg gcaagccgga gctcgcccga aaaatcgaaa cgtcggcgaa
      601 cttcacgccc ggtaatttcg aaagccacgc tcagaccggc ttcggcgagt tcggtctgca
      661 gccgtttcga ctcggctaaa tcaatttttt caaacacgtc gaaagcggcg atttgcagaa
      721 aactctttgt ccattcgatt cgcaaagcgg caagctcgtc gtgaaaattc gcctgccgac
      781 gaaccgccgc gcccagattt tcggcataat cgaccgcttc gaaatcttcc gtctccagcg
      841 gcaaagattc gattagcttc ggatttgccg cgatcatctc ggtaaaggac ggcgaaatcc
      901 ggcagaattt ttttaaagcc gccagctttt tctcggtcaa atcaaacggc gaatcggatt
      961 tttccagcgc ggaaacgatt tgcagcggca ggtccggcgt ctgcccggcg agcgcgcgaa
     1021 cgcgaaccgg cgagtcaaat gaattcaagg tcgaatttaa cccgaatcga ttagcgttct
     1081 gtcgagcgcc gcgggcagtc gccagacctt cctccgtaac attttcggca tcgccggttt
     1141 cctcaaaaag gcgcgcgaaa atccggttta cgctttccgc gtgtttattc agctccgcat
     1201 cgaagctttc cggcgacgga caattcattc ttttagcgat tacccggcgt ttttccgcgt
     1261 cttccggcac gagatgcgtt tgcagaccgt gttccatttg cagtcgatgt tcgagccggc
     1321 gcagaaaatc gtaagcatcg gcaagctcgg tcaaatcgtt ttcggtcaac agcttgcggt
     1381 ctgccaggcg cgaaaggcta atcagcgtgt gcggcgcgcg cagccaggcg tcgcgggcgc
     1441 cgtaggcaat ctgcaaagcc tgcgcgataa attcgatttc gcggatgccg cctttgccga
     1501 gcttgacgtt aaagcctctg ccggaaagct tttcgagatt gattttttct ttcgagaggc
     1561 gaacgtttct gagagcgttt tcaaccgttt catcttttga aaaaacgctg gtttcgatgc
     1621 tcgcaaaaaa acttttgaaa atctctgcgt cgcccgccgc cgagcgcgag cgaatcatcg
     1681 tctgccgctc ccaggcttgc gccgatgtcc ggtaataatt aaccgcgtcc cgcacggaaa
     1741 ttgccagcgc gccgacgcgc ccgtgcgggc gaagccgcaa atcgacgcga tacgccgcgc
     1801 cctcgccgga agattgaccg ccgacgagct tgacgataaa ttccgataat ttcacaaaat
     1861 attcgcggtt cgtgaccgcg ccgcgcgagc cttgaccgga agtcgagcct tcggacgaat
     1921 aaagaaaaag caaatcgatg tcggacgact aattaagctc gccggagccg agctttccga
     1981 gcgcgacgac ggacaa
//
LOCUS       DASWNE010000357         5157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_505083, whole genome shotgun sequence.
ACCESSION   DASWNE010000357 DASWNE010000000
VERSION     DASWNE010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505083"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            408..1658
                     /locus_tag="VGB68_07240"
     CDS             408..1658
                     /locus_tag="VGB68_07240"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY0049062.1"
                     /translation="MNSIRTAFIITSLLTVVGAACAQTPPRTAPSPNNIQSKCAPLET
                     RQANAPEQRPAFAGQTRACAAKSDVAFNVEVLAKGLEKPWSVEPLPTGDLLVTEKPGR
                     LRIVSAKGELGQPIGGLLPVDARGQGGLLDAALSPDFASDRTIYWSFSEPREGGNATS
                     VARGVLSADRSKLEQVRVIFRAMPTYEGTMHFGSRLAFGTDGMLYITLGERSNPAIRP
                     QAQQLSSHMGKTIRIRPDGTVPPDNPFVGRKDALPEIWTLGHRNMQASAFDAQGKFWV
                     VDMGPQGGDELNLIEKGKNYGWSVVTYGEEYSGAPIPNAVTAREGFEQPVYYWDPVIA
                     PSGAQFYTGDAFPSWRGSLFIGALREKRLVRLVIEGNRVTGEEHLLADRNQRIRDVRQ
                     GPDGALYIVTDQENGELWKISPRR"
     gene            1972..3783
                     /locus_tag="VGB68_07245"
     CDS             1972..3783
                     /locus_tag="VGB68_07245"
                     /inference="COORDINATES: protein motif:HMM:NF016915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049063.1"
                     /translation="MKSLKKSISFLLVLVFLIGGVSAFAQTRRKAAKPAPGKEATAVN
                     QTKTNPKKCDGGWSGTITYSKTQNDSEHRKTPTGYQSSSSSFKATAEVAVNEDGSAKA
                     SVKVRVSSNLEDVKEGSECCMISLAGCTRNGSFKYADIIKTDSSAEASASVKGVKISG
                     ETFTADIGVPEAIGKTLRTIRTIRKRECDKLNQDASHDQEAAASYLVGPIQISGTIDP
                     NNPNVIRGTKTDGDVTITYNLARCAAPDIKLADLALEHHIFPDAKAWHAVNSETIDGN
                     LVRIKAKVRNDGTAQGYATVKFTETSENIELPGSASISLQPGEEREVEYEWDTSGFAW
                     TNDKKPKSARKIKAEIENDSLTEDIKIIPKPVILVHGLWSNAAAWSNYQSYLNEAHTF
                     AWKAFPVGADPAHGLMNTGNSFGNNQPTNSIFENARELGKQIEHAQKSMNAWHVDVVA
                     HSMGGLISRFYIHHLMKDSFDGKPVVTHLAMLGTPNMGSPWADIMFEEYKEKGYHVEA
                     LRELKTDVCRIFNSQVTNRKGVKFSITYTDKIPLTGNTAEAGDGVVSKSSAIWQIDDV
                     SHSGSLDHTSLTGKEDFMQFIYPRLAVGPSGNHQAEK"
     gene            4046..4261
                     /locus_tag="VGB68_07250"
     CDS             4046..4261
                     /locus_tag="VGB68_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049064.1"
                     /translation="MNQNNLTETIDKSIEELGFARIPRIAVTETFSSGEPVSFETNEK
                     LREFAEKQGLEIREEDDFVIFERKAAE"
     gene            4322..>5157
                     /locus_tag="VGB68_07255"
     CDS             4322..>5157
                     /locus_tag="VGB68_07255"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HEY0049065.1"
                     /translation="MTNTAEQIVDERLRSLIESEKQLLKDLQELAATGEHAEDMRQLK
                     DILGGIDELFLLVVVGEFNSGKSSFINALFGQKIRVEGPVPVDDHITILRHGEQPEER
                     SLSSFVTQKLAPIDFLRNISIVDTPGTNSVIRQHQEITEDFIPRADLILFITSIDRPL
                     TESERQFLSFIQNWGKKIVIVLNKIDTKEEAEIAEVIEFVDGKCRELLGFKPLIFPVA
                     AKLALNAKLGSNPRDWTRSRFEALEDYILNKLGERERLQLKLLSPLDSARTIAGKLQS
                     EYT"
BASE COUNT         1421 a         1274 c         1329 g         1133 t
ORIGIN      
        1 ctgccgttaa ctcaaaaaag cgcttttttg agttaacggc agaatatccc gcgccgaagc
       61 atctatagag aaaggctccc attataagaa acgttctcaa aagaatcgtt tttgagactg
      121 taacgttgcg ttttagcagc cttcaaagct tcaaccaatc ccagcaccgg aattacatta
      181 aaaaacaaat aaatcaggat aaaaaaccaa atctatttta gcctccgcgg cgaatcagcg
      241 gacttgaggt tttgaagctt taatttcgag ccggctttcg gaacggctcg gaaaaacggg
      301 tcgattgcgg cttcgggcat aattgtaatt caatttggaa tggacacgcc tttgtctaat
      361 cgttcatact tataattata gagattataa acaaggaggg ttaatccatg aatagtatcc
      421 gaacagcctt tattattacg agtcttttga ccgtcgtcgg cgcagcctgc gctcagacgc
      481 cgccgcgaac cgcgccgagc ccgaacaaca tacaaagtaa atgcgcgccg cttgagaccc
      541 ggcaggcaaa tgcgcccgag cagcgtccgg ctttcgccgg gcagacgcgc gcctgcgccg
      601 ctaaatcaga cgtcgccttt aatgtcgagg tgcttgccaa aggactggaa aaaccatggt
      661 cggtcgagcc cttgcctacc ggcgacctgc tcgtgacgga aaaaccgggt cgtttgcgca
      721 tcgtctccgc taagggcgaa ttgggtcagc cgatcggcgg tctgctgccg gtcgacgcgc
      781 gcgggcaggg cggtttgctc gatgctgcgc tgagcccgga ttttgcttcc gaccggacaa
      841 tttattggag tttctcggaa cctcgcgaag gaggcaatgc gacgagcgtg gcgcgcggag
      901 ttttatcggc agaccgctcg aaactggaac aagtgcgagt gatatttcgg gcgatgccga
      961 cttatgaagg cactatgcac ttcggttcgc ggctcgcctt cggtacggac ggaatgttgt
     1021 atataacgct gggcgagcgc tcgaacccag ccatccgccc gcaggcgcag cagttaagca
     1081 gtcatatggg aaaaaccatc cgcattcgcc ccgacggcac tgtgccgccc gacaatcctt
     1141 ttgtcggacg aaaagacgcg ctgccggaga tttggacgct cggtcacaga aatatgcagg
     1201 cgtcggcgtt cgacgcgcag ggaaagttct gggtagtgga tatgggaccg cagggcggcg
     1261 acgaattaaa cctgattgag aaagggaaaa attacggctg gtcggtggtt acatacggcg
     1321 aggaatattc cggcgcgccg attccgaatg ccgttacggc acgcgaagga tttgagcagc
     1381 ccgtttatta ttgggacccg gtcatcgcgc cgtccggcgc acagttctat acgggcgacg
     1441 cgtttccgag ctggcgcggc agtctcttta tcggggcgct cagggaaaag cgactggtgc
     1501 ggctggttat cgagggcaat cgcgtcaccg gagaagaaca tttgctggct gaccgcaatc
     1561 agcgaatccg cgacgtcagg cagggaccgg acggagcatt atatattgtc accgaccagg
     1621 aaaacggaga gctttggaag atttcgccgc ggcgataaag ggaaaacgaa acgcgagtta
     1681 aaaacattga tagccgcgtg tagaaattgc tgcgctttaa ctattttgaa tagaataaac
     1741 gtaaagagtc ggaaagcttt agttttccga ctcttttatt tgttttagag aagtcgttaa
     1801 aaaaatcatt accggagcgc gtctcaaaaa cgaccctttt tgagccgaat aaaaacctgc
     1861 ctgaaagtat cgattctgaa cggttcttcc tgatactttt tcacaaaatt ttacgcctta
     1921 caaattgaag acaaatttta cgtttacata aattcgcctt aaaaaatgca attgaaatct
     1981 ttaaaaaaat caatctcttt tcttttagtt ttagtttttt taatcggcgg cgtttcagcc
     2041 tttgctcaaa cgcggcgcaa agctgcgaaa ccggctccgg gcaaagaagc gaccgcagtt
     2101 aatcagacga aaaccaatcc taaaaaatgt gacggcggct ggagcggcac gattacctat
     2161 tccaaaaccc aaaacgacag cgaacacaga aaaactccga ccggttatca aagcagcagc
     2221 tcgagcttta aggcgacggc ggaagttgcc gtcaacgagg acggctcggc gaaagcgagc
     2281 gtgaaggtca gggtttcgag caatctggaa gacgttaaag aaggctcgga atgctgtatg
     2341 atttcgcttg ccggctgcac gcgcaacggc agcttcaaat atgcggacat tattaaaacc
     2401 gactccagcg ccgaagcgag cgcgagcgtc aaaggcgtga agatttccgg cgaaactttc
     2461 accgccgata tcggcgttcc ggaagcgatt ggaaaaacgc ttcgaaccat ccggacaatt
     2521 cgcaaaagag agtgcgataa gcttaatcag gatgcgtcgc acgaccagga agctgcggct
     2581 tcatatctgg tcggaccgat tcagatttcc ggcacgatcg acccgaacaa cccgaacgtc
     2641 ataagaggaa cgaaaaccga cggcgacgtg acgattactt ataacctggc gcgctgcgct
     2701 gcgccggata ttaaactggc ggatttggct ttggagcatc atatctttcc cgacgcgaag
     2761 gcttggcacg ccgtcaattc cgaaacgatt gacggcaatc tcgtgcgcat caaggcaaaa
     2821 gtgcgcaacg acggcacggc acagggttac gcgaccgtta aatttaccga aacgagcgaa
     2881 aatatcgagc tgccgggttc tgcttcaatt tccctgcagc cgggcgaaga acgcgaggtc
     2941 gaatacgaat gggacacttc cggttttgcc tggacaaacg acaaaaagcc gaaatcagcg
     3001 cgtaaaatca aagccgaaat cgaaaacgac agcctgaccg aagacatcaa aatcatcccg
     3061 aaacccgtca ttctggttca cggtctgtgg agcaatgccg ccgcctggtc aaattatcaa
     3121 agctatctga acgaagcgca cacgtttgcc tggaaagcct ttccggtcgg agccgacccg
     3181 gcgcacggtt tgatgaacac gggcaattca ttcggaaaca atcagccgac gaactcgatt
     3241 ttcgagaacg cgcgcgagct cggcaaacaa atcgagcacg cgcaaaaatc catgaacgcc
     3301 tggcacgtcg acgtggtcgc gcattcgatg ggcggtttga tttccagatt ttacattcat
     3361 catttgatga aggattcatt tgacggaaaa cccgtggtga cgcatctggc gatgctcggc
     3421 acgccgaata tgggaagtcc gtgggcggat attatgttcg aagagtataa agaaaagggt
     3481 tatcacgtcg aggctctgcg cgagctgaaa acggacgtct gccgaatttt taacagtcag
     3541 gtaacaaatc gaaagggcgt gaaattttcg attacttata cggacaagat tccgctgacg
     3601 ggaaataccg ccgaagccgg cgacggcgtg gtgtcaaaat cttcggcaat ctggcaaatc
     3661 gacgacgtca gtcattccgg cagcctcgac catacgtctt taaccggcaa agaagatttt
     3721 atgcaattca tttatccgcg cctcgccgtc ggacccagcg gaaatcacca ggcggaaaaa
     3781 tagccggact atgaaaataa acaattaaac acacaggtcg cgtaaattaa ttgtcttctt
     3841 cgagcttcca tttcagcgcg gcgaaaaggc tggaaaagcg tcgctgacgg gcataataat
     3901 gaggcgggta aatcctgcct tctttttttt tcgccagtat caggcttgat tcgctcgcac
     3961 cggattgtcg aaatttgtaa aaattaccgg ctgttacgtg ttaaaatgtc taaactaata
     4021 atttacaatc taaaaagggg aaacaatgaa tcaaaacaat ttaaccgaaa ccatcgacaa
     4081 gtctatcgaa gaattagggt ttgcgcgcat accgagaatt gccgtgaccg aaactttctc
     4141 gtcgggcgag cctgtttcat tcgaaaccaa tgaaaagctc agagaatttg ccgaaaaaca
     4201 aggcttggaa atcagagagg aagatgattt tgtgattttc gagcgtaaag cagccgaata
     4261 attgatgctt tgaacagata aaacagcaac gtaaaatgag cggctcattt attataggct
     4321 tatgacaaac actgccgaac aaatcgtcga cgagcgtctg cgttctttga tcgaatcgga
     4381 aaagcaactg ttaaaggacc ttcaggaact tgccgccacg ggcgagcacg ccgaagatat
     4441 gcggcagttg aaagacattc tcggcggaat cgacgagttg tttctgctgg tcgtcgtcgg
     4501 cgaattcaat tcgggcaaat cttcgtttat caacgctttg ttcggtcaaa aaattcgcgt
     4561 cgaaggacca gttccggtag acgatcacat cacaatcctg cgtcacggcg aacagcccga
     4621 agaacgctct ctgagctctt tcgtcacgca gaagctcgcg ccaatcgatt ttttgcgcaa
     4681 tatctcgatt gtcgatacgc ccggaacgaa ttcggtcatc aggcagcatc aggaaatcac
     4741 cgaagacttt ataccgcgcg ccgacctgat attattcatc acctccatcg accgcccgct
     4801 gaccgaaagc gagcgccagt ttctttcctt catccagaac tggggcaaaa aaatcgttat
     4861 cgttttaaat aaaattgaca ccaaagaaga ggctgagata gccgaggtca tagagtttgt
     4921 cgacggtaaa tgccgcgagc tgttgggctt taagccttta atcttccccg tcgccgccaa
     4981 gctggcgctc aacgcaaaac tcggaagcaa tccgcgcgac tggacgcgca gccggttcga
     5041 agcgctcgaa gattacattt taaataagct cggcgagcgc gaaaggcttc agttgaaact
     5101 actcagcccg ctcgattccg cccggacaat tgccggaaaa ctgcaatcgg aatatac
//
LOCUS       DASWNE010000358         3683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_12009, whole genome shotgun sequence.
ACCESSION   DASWNE010000358 DASWNE010000000
VERSION     DASWNE010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12009"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VGB68_07260"
     CDS             complement(<1..285)
                     /locus_tag="VGB68_07260"
                     /inference="COORDINATES: protein motif:HMM:NF022577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HEY0049066.1"
                     /translation="MNNKNESITDFLTNMENNVGTPERVISSIGGGALLLYGIRRGGV
                     LGILASVAGGGLALRGVTGHCHLYDKFNIDTAEKGDNASPFHKGFLATKIH"
     gene            complement(309..1352)
                     /locus_tag="VGB68_07265"
     CDS             complement(309..1352)
                     /locus_tag="VGB68_07265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0049067.1"
                     /translation="MSENNNKWLLGTAVGVGAIAAFTLLGKSRRRFDFDKKVVLITGG
                     SRGLGLVLARELAQEGARLAICARDGDELARAREELQAAGAEVFDVICDLREQEQVKR
                     MIEDINTRYGQIDVLINNAGVIQVGPLEVQTQDDFEKAMAIHFWAPFYTMQAVLPQMK
                     RRGEGRIVNITSIGAKVAVPHLAPYCASKFALLGLSNAMRGELAKDNIFVTTVCPGLM
                     RTGSHINAIFKGKNEIEFALFSLMDALPLTSISAERAARQIIAAAARGDAELIITTQA
                     KMAAKANALFPETVAQVMGLVNRLLPSAGGIGTSHALGKDSTSFASPSILTALADKAS
                     IKNNELEPNEYLA"
     gene            complement(1713..>3683)
                     /locus_tag="VGB68_07270"
     CDS             complement(1713..>3683)
                     /locus_tag="VGB68_07270"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0049068.1"
                     /translation="DIPDELLVQGAVDREHLQIMRQIGFRSAMIVPLKARGKVLGVLS
                     FVNSESGKHHTPGDLVLAEDLASRAAMAIDNAQLFRAEHQTRRAAERTSDFLRRLQMV
                     SSSLSQALTPNDVAVAVIEQGVNSLGAHAGTVVLLNPATAELEIVGTVGFPEELTKKW
                     QRFHLHQKVPIADAIRERKPVLVESFAAHMDKYPGLGPLASVTGSQALGAFPLIIEGR
                     TIGAMGLSFPRVQVFSEDDIAFMLALAHQCAQALERARLYETEQRLRAQAETANRIKD
                     EFLATVSHELRTPLNAIVGWSSLLMTDTLDESVKARAIETIGRSAKSQSQIIEDLLDV
                     SRIITGKLSLNTSLTELETIIKTALESLYPAAESKNIEIKSKFEFPSKFIWGDADRLQ
                     QIMWNLLSNAIKFTPKGGEIRVSLEQVESHIQIKVSDTGQGINKEFLPFVFDRFSQAD
                     GTSTRKFGGLGLGLAIVRHLVEMHGGTVEVESEGLNQGTTFTITLPIKAAFDNDPADA
                     DLPRADGKAAVVNFAHNLSGVRVLIVDDDTDARLLLTTIIEKSGASIVTAASVSEALE
                     TLKSFEPHILISDIGMPGEDGYSLIRKLRALYPEKAGKISAVALTAYARQEDKTLALE
                     AGFQIHVAKPVNPEELLSLLKELSEAERLDEK"
BASE COUNT          760 a         1020 c         1001 g          902 t
ORIGIN      
        1 gtggattttc gttgccagaa aacctttgtg aaacggcgaa gcgttgtcgc ctttttcggc
       61 ggtgtcgata ttgaacttgt catacaaatg gcagtgtccg gtgacgccgc gcagggcaag
      121 cccgccgccg gcgaccgaag ccagaatacc gagcacgccg ccgcgcctga tgccgtagag
      181 cagcagcgcg ccgccgccga ttgacgatat aacgcgttcg ggcgtgccga cgttgttttc
      241 catattcgtt aaaaaatcgg ttatcgattc gtttttgttg ttcataaatt atcccttcct
      301 tgtctgaatt aagctaaata ttcgtttggt tcgagctcgt tatttttgat tgaagctttg
      361 tccgccagcg cggtcagaat cgaaggcgaa gcaaaagacg tgctgtcttt gccgagcgcg
      421 tggctggtgc cgatgccgcc cgccgaaggc agaagccggt tgaccaagcc cattacctgc
      481 gcgacggttt ccggaaacag cgcgttggct ttcgccgcca tcttcgcctg cgtcgtgata
      541 atcaattccg catcgccgcg cgcggctgcc gcgataatct gccgcgccgc gcgctcggcg
      601 ctgatcgaag ttaaaggcag agcgtccatc agactgaaaa gcgcaaactc gatttcgttt
      661 ttgcctttga aaatcgcgtt gatgtggctt ccggtgcgca tcaagccggg gcaaacagtg
      721 gttacaaaaa tattgtcttt ggcaagctcg ccgcgcatcg cgttcgacaa gccgagcagg
      781 gcgaatttgc tggcgcaata cggcgccaga tgcggtacgg cgaccttcgc gccgattgac
      841 gtgatgttga cgatgcgtcc ctcgccgcgc cgtttcatct gcggcaaaac cgcctgcatc
      901 gtgtaaaaag gcgcccagaa atgaatcgcc atcgcctttt caaaatcatc ctgcgtctgc
      961 acttccagcg gtccgacctg tatgactccg gcgttattaa tcaaaacgtc aatctgcccg
     1021 tagcgcgtgt tgatgtcttc aatcattcgt ttgacctgct cctgctcgcg caggtcgcag
     1081 ataacgtcga aaacttccgc gccagccgct tgcagctctt cgcgcgcccg cgccagctca
     1141 tcgccgtcgc gcgcgcagat tgccagccgc gcgccttcct gcgccagttc gcgcgccaga
     1201 accagtccga gcccgcgcga gccgccggtt atcaaaacga cctttttatc aaaatcgaat
     1261 cggcgtctgc tcttgcctaa aagcgtgaaa gccgcgatgg ctccgacgcc gacggctgtg
     1321 cctaaaagcc atttattgtt attttcggac acaaagattt ttccttttaa taaatttgta
     1381 ttgaaacttg tattgaaaca aaacaaaaaa ttgttatttg agtcagattt ggcaagaatc
     1441 gtgccgtgcg ttcgggcagg gaatttttat cgttgcgagg ctgagagaga tgaacgcgga
     1501 gtgatgcagt caaaacgttg aacgcggaac cggaaattaa ttattcggcg tttattattg
     1561 tgaattgctt ttttgcttta cgaagtatgc cctgacaccg gcatcgtaaa attattgacc
     1621 ggtacggcgg atgttttcga ttagctcgcg atgtttgccg cctttttggc aaatgaagcc
     1681 cgcgtaattt gccaaacagc ggcttgagta atttattttt cgtctaatct ttcagcttcc
     1741 gacagctctt tcaggagcga gagcagttct tcggggttga ccggcttggc gacgtgtatt
     1801 tgaaagcccg cttccagcgc cagcgttttg tcttcctggc gcgcgtatgc ggtcagcgcg
     1861 acggcggaga ttttaccggc tttttccggg taaagcgcgc gcagttttcg aatcagcgaa
     1921 tagccgtctt cgcccggcat tccgatgtcg ctaatcagga tgtgcggctc gaagcttttt
     1981 aaggtttcca gcgcttcgga aaccgaggct gccgtcacga tactcgcgcc gcttttttcg
     2041 atgatggtcg ttaataacaa tcgcgcgtcc gtgtcgtcgt cgacgataag gacgcggacg
     2101 ccgctcaaat tatgagcaaa attaacgacc gccgcctttc cgtccgcccg cggtaaatcc
     2161 gcgtccgccg ggtcattatc aaacgccgct tttatcggca gcgtgatggt aaaagtcgtt
     2221 ccctgattga gcccttcgct ttcgacctcg accgttccgc cgtgcatttc gaccagatgc
     2281 cggacgattg ccaagcctaa gcccaagccg ccgaattttc gcgtcgaggt gccgtccgcc
     2341 tggctgaaac ggtcgaaaac aaaaggcaga aattccttgt tgatgccttg tccggtgtcg
     2401 ctgactttga tttgtatgtg agattcgacc tgctccagac tgacacggat ttcgccgcct
     2461 ttcggcgtaa atttgatggc gttcgacaac aaattccaca ttatctgctg aaggcggtcg
     2521 gcgtcgcccc agataaattt cgacggaaat tcgaatttcg acttaatctc gatgtttttt
     2581 gattcggctg ccggatacag gctttccaga gcggttttga taatcgtttc aagctcggtc
     2641 aagctggtgt tcaagcttaa tttgccggtg attatacgtg aaacatcgag caaatcttcg
     2701 ataatctgcg actgcgattt ggcgctgcgc ccgattgttt cgatggcgcg cgcctttacg
     2761 ctctcgtcca gcgtatcggt catcagaagg ctcgaccagc cgacgatggc gttcagcggc
     2821 gtgcgcagtt cgtgcgatac ggtcgccaga aattcgtcct tgatgcggtt ggctgtctca
     2881 gcctgcgcgc gcaatctctg ctcggtttcg taaagccgcg cgcgttccag cgcctgcgcg
     2941 cattgatggg cgagcgccag cataaaggcg atgtcgtctt cgctgaaaac ctgcacgcgc
     3001 ggaaagctta aacccatcgc gccgatagtg cgcccttcga taatcagcgg aaacgcgccc
     3061 agcgcctggc tgcccgtgac cgaagcgagc ggtccgagtc cgggatattt atccatatga
     3121 gcggcaaaag attcgaccag aaccggtttc ctttctctta tggcatcggc aatcggcact
     3181 ttttgatgca aatggaagcg ctgccatttt ttcgtcagtt cctccggaaa accgaccgtt
     3241 ccgacgattt ccagttcggc ggtcgcggga ttgagcaaaa cgaccgtgcc ggcatgcgcg
     3301 ccgagcgagt tgacgccttg ttcgataacg gcgacggcga cgtcgttcgg cgtcagggct
     3361 tgcgaaagcg agctggaaac catctgcaga cgccgtagaa aatcggacgt ccgctcggcg
     3421 gcgcgccggg tttgatgctc ggcgcggaaa agctgcgcgt tatcgatcgc catcgccgcc
     3481 cggcttgcca aatcttcggc taaaacgagg tcgcccggcg tgtgatgctt gccggattcg
     3541 gaattgacga agctgagaac tcccagaacc ttgccgcgcg ctttcagcgg aactatcatc
     3601 gccgagcgaa agccgatttg ccgcattatc tgtaaatgct cccggtcgac cgcgccctgc
     3661 acgagcaatt cgtcgggaat atc
//
LOCUS       DASWNE010000359         1589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_647252, whole genome shotgun sequence.
ACCESSION   DASWNE010000359 DASWNE010000000
VERSION     DASWNE010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647252"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VGB68_07275"
     CDS             <1..267
                     /locus_tag="VGB68_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049069.1"
                     /translation="VADGRISPAKGGRVPGKNNRYTIVLLNDEAELKYIQQRRVPGGF
                     VDDICDRFPQVEAVLENSVGTLGLTLEPSAPRPRRVGSLRLALE"
     gene            503..775
                     /locus_tag="VGB68_07280"
     CDS             503..775
                     /locus_tag="VGB68_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049070.1"
                     /translation="MKKIKQITKMLLVSALFATVVFGGDLRNEDGRRYEVKIHANSTT
                     MNTYIDANTTTLSVCSDCEIEVVGVGKIRIKGSERAVIKNGKLFKQ"
     gene            802..1377
                     /locus_tag="VGB68_07285"
     CDS             802..1377
                     /locus_tag="VGB68_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049071.1"
                     /translation="MSNRNLSKIGFLLFAALFAAACGGKSNATGEVVPIESVCAYQKA
                     QPVAVEGFLAPNTMRCEIVRGKRTSSISGCTFNVYANSSRTGASVPVFISADGWYGGK
                     NNRIVNPSNYPGDLQFTDKNGNPPPKKGPEIYDNDGNPIPPGSKIRVYSKLPNSDKCE
                     FRLAERIERVEDEQSARNVEKNIRNLTVESF"
BASE COUNT          405 a          416 c          440 g          328 t
ORIGIN      
        1 gtcgccgacg ggcgcatcag tccggcaaag ggcgggcgcg tgcccggcaa aaacaatcgc
       61 tatacgattg tgctgctcaa cgacgaagcc gaattgaaat acattcagca gcgccgcgtg
      121 ccgggcggct tcgtcgacga catctgcgac cgttttccgc aggtcgaagc cgttctggaa
      181 aattcggtcg gcacgctcgg cttgacgctc gaaccttccg cgccgcgccc gcgccgcgtc
      241 ggctcgctgc ggctcgcgct ggaatagctt gaaacgcgac gagcgcgaag cgataaatcc
      301 gcgattttgc gttcgtcgct tcgcgctcat cgcgtttaaa aactgcccgc gtttgcgccg
      361 tatcaaaaaa ttctcccgct cgtgtccggt ttcaaatacg gttttcgtct ttatggctga
      421 ggacgaaaac gcgttctcgg aaaattcata ttcggcgagc cggggaagtt aataaatttt
      481 aatacttaaa ggagcaatca aaatgaaaaa aatcaaacaa ataaccaaaa tgctgctggt
      541 ttcggcgctt ttcgctacgg tcgttttcgg cggcgatttg cggaacgagg acggcaggcg
      601 ctacgaagtc aaaattcacg ctaactcgac cacgatgaac acttatatcg acgccaacac
      661 gacgactctg agcgtttgca gcgactgcga aatcgaggtc gtcggcgtcg gcaaaatcag
      721 aatcaagggt tcggagcgcg cggtcattaa aaacggcaag ctgtttaaac agtaattgtc
      781 cgaaatcgaa aggtgaaaat tatgagtaac agaaatctat cgaaaatcgg ttttttactt
      841 ttcgccgcgc ttttcgccgc agcctgcggc ggaaaatcga acgcgaccgg cgaagtcgtt
      901 ccgattgaaa gcgtctgcgc ctatcaaaaa gcgcagcccg tcgccgtcga gggctttctc
      961 gcgccgaaca cgatgcggtg cgaaatcgtg cgcggcaaac gaacgagcag catttcgggc
     1021 tgcactttta acgtctacgc aaacagcagc cggacgggcg cgagcgttcc cgtttttatc
     1081 tcggcggacg gctggtacgg cggcaaaaac aaccggattg tcaacccgtc aaattatccg
     1141 ggcgatttgc agtttaccga taaaaacgga aatccgccgc ccaaaaaggg accggaaatt
     1201 tacgacaacg acggaaatcc gattcctccg ggcagcaaga ttcgcgttta cagcaaactg
     1261 ccgaattcgg acaaatgcga atttcgtctg gcggagagaa tcgagcgcgt cgaagacgaa
     1321 caaagcgcgc gaaacgtgga aaagaatatt cgcaacctta cggtcgaatc tttctaactg
     1381 acggcaaata aatggtttgc gtccctgcgg tttccagatg cggcgcaggg aattcggatt
     1441 gaagcggtgg aggctcggct ggaaggcgac acggatttgc gcggctttct cggcttggac
     1501 gaaaccgtgc gcaacggctt ggataaaatc cgtataactt tcagcgtcga gtcggatgcg
     1561 ccgcgtgaaa aaatacgcga gctcgtcac
//
LOCUS       DASWNE010000360         3056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_12092, whole genome shotgun sequence.
ACCESSION   DASWNE010000360 DASWNE010000000
VERSION     DASWNE010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12092"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2353)
                     /locus_tag="VGB68_07290"
     CDS             complement(<1..2353)
                     /locus_tag="VGB68_07290"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0049072.1"
                     /translation="MSFKKILKIVLVFTFLFIASQEIRAQYAYGLSDIVYDNTTGKVT
                     SLSRTSLDYWAGLHYDPAVVGGVFQEGQLLGYSSHTGYANVFSALVSLRTVNSAPPDT
                     RYDVLSDHYIVAYFYTTVIIWDDETPVPVRRYYDPFGFGSFGGGSEPGWSSFKGSAAR
                     ESFWEYQYFYLGTTGKGIIANPTVCHLPLAENNAAPCPTPTPITVQITNRFKPNQDLS
                     ASSEVQQALVGASVDLRAVVSPESSQTTYRWTSASSLGDQCNTNSLCSVKFNQPTQDN
                     QYHTINLEVRQGSQPPVTKSVRIKVSMPTITYTGTERMPRVTTSSNPCVVGILTNAQN
                     LILGCPDTIPATRNSIDDYGLYAKAVVTPPTGFISDGTTSYVEFRQLVKPARKSFGRN
                     VSECLVFDTAGQDGWREDFNGRDPELFPEFLAAGFKLRSTFRNNATGTNADAILEFLD
                     SPGTVLANTNANNVNLNKYTIDDQFETYVTYFAGLEDSENLGDEYRHALAVMPWKWSG
                     AAEKRSSGWQLVGAKFPQTEPTPVGSELKTFSLTSTGERAFSGTFRNDNERYESCSSK
                     PPVNIRTKRNIAVWRNGIWYVMNEHGGTQTVIQWGVDYDIPVPGDYDGDGISDFAVYR
                     PDNPATPEDECQFGGGCAWYIIKSAGGSLYGYQLGAKDDKPVPADYDGDGKTDMAVFR
                     PSNAGWYQVRSSDNTFYGIQFGQFNDVFVSSDFDGDGIDDLSVWSPTNAAWTILNSSN
                     QAVTSQQWGFSTDKPVIGDYDGDGKTDIANWQADGLWHILLSSN"
     gene            complement(2384..>3056)
                     /locus_tag="VGB68_07295"
     CDS             complement(2384..>3056)
                     /locus_tag="VGB68_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049073.1"
                     /translation="LLVMAVGFIVTATNSITRVSGQGETPSPTPTPRLNLYTDAKPMP
                     PELAYWALFQEIQALKDKDGESLAQSERTNFKNSFYTNRLGLESSQFAAVDTAATECL
                     ANIQPIDQSAREIIKKYRAQFPNGELKKIQPSSPPNKQQPARPQKSFESLPPVPAELK
                     QLQNQKNQIILNAKEKIKQSLGQAEFAAFDSSVQKNAARVLIPLNLANKVLPPLATPT
                     PTPGN"
BASE COUNT          713 a          758 c          724 g          861 t
ORIGIN      
        1 aattgctgct caacaggata tgccagagcc cgtctgcttg ccagttggcg atatccgttt
       61 tgccgtcgcc gtcataatct ccgataaccg gcttatccgt cgaaaatccc cactgctgac
      121 tggtaacggc ttgattactg ctgttaagaa tcgtccatgc tgcattagtc ggcgaccaga
      181 cgctcaggtc gtcgatgccg tcgccgtcga aatcgcttga tacaaacaca tcgttaaatt
      241 gtccgaactg gattccgtaa aatgtgttat cgctgcttct tacctgatac cagccagcgt
      301 tgctcgggcg gaagactgcc atatccgttt tcccgtcacc gtcgtaatcc gcaggaacgg
      361 gcttatcatc tttcgcgccc agttgatagc catataaact gccaccggcg cttttgatga
      421 tataccaggc gcatccgccg ccaaactgac attcgtcttc aggcgttgcc ggattatccg
      481 ggcggtaaac ggcgaaatcc gaaataccgt caccgtcgta atcgcctggc acgggaatat
      541 cgtaatcgac tccccattga atcactgtct gtgttcctcc gtgctcattc atcacgtacc
      601 agatgccgtt ccgccacacg gcaatattcc gctttgttct aatattaacg ggaggctttg
      661 agctgcagct ctcataccgt tcgttgtcat ttctaaaagt tccgctaaaa gctcgctcgc
      721 ccgttgaggt taagctgaat gtctttaatt cgctaccgac cggcgtgggt tcggtttggg
      781 ggaatttagc cccgaccaat tgccagcctg agcttctttt ttcagccgcg ccgctccact
      841 tccagggcat tacggcaagc gcgtggcgat attcatcgcc cagattctca ctatcttcaa
      901 gtccggcaaa ataggtcaca tacgtttcga actgatcgtc gattgtatat ttattaagat
      961 ttacattatt ggcattcgta tttgctaaaa ctgttccggg cgaatctaaa aactctaata
     1021 tagcatcggc attcgttccg gttgcattat ttctaaaggt gcttctaagt ttaaagccgg
     1081 ctgccaaaaa ttctggaaat agttcagggt ctcgtccgtt aaaatcctcg cgccagccgt
     1141 cctgtcccgc cgtgtcaaaa accaaacatt cgctgacatt tcgcccgaag cttttgcggg
     1201 ctggttttac aagttggcga aactcaacgt aggacgtggt tccatctgaa ataaatccgg
     1261 tcggcggagt gactaccgcc ttcgcgtata agccataatc atcaattgaa tttctggtag
     1321 caggaattgt atcgggacac cctaatatca ggttttgagc atttgttaga ataccgacga
     1381 cacaaggatt gctcgaggtc gtcacgcgcg gcatgcgctc agttcccgta taagttattg
     1441 tcggcatcga cacttttatt ctgacggatt ttgtaaccgg cggttgactg ccttgccgaa
     1501 cttcaagatt tattgtgtga tattgattat cttgtgtcgg ctggttgaac tttacgctgc
     1561 acagactgtt tgtattgcat tggtcgccga gactgcttgc gctcgtccaa cggtaagtcg
     1621 tttgtgacga ttcaggcgat acgacggctc ttaaatcaac cgacgcgccg actaaagcct
     1681 gctggacttc gctgctcgcg ctcaaatcct gattaggctt aaatctgttg gtaatctgta
     1741 ctgttatcgg cgtcggcgtc ggacagggag cggcgttatt ttccgctaag ggtaaatgac
     1801 atacagtagg gtttgcgatg attcccttgc cggtagtgcc gagataaaag tattgatatt
     1861 cccaaaagct ctctcttgct gccgaacctt taaaactcga ccatcccggt tcggatccgc
     1921 cgccgaaaga accaaagccg aacgggtcgt aatacctgcg cacgggcacg ggtgtctcat
     1981 catcccaaat aatcaccgtg gtataaaaat aggcgacgat gtagtggtcg ctcaaaacgt
     2041 cgtagcgagt atcgggcggt gctgaattta ccgttcttaa actgacgagc gcgctgaaaa
     2101 cattagcgta gcccgtgtga gagctgtatc ctaataattg tccctcttga aacactccgc
     2161 cgaccacagc cgggtcgtaa tgtaagccag cccagtaatc cagcgaagtt ctggaaagac
     2221 tcgtaacttt tcccgtcgta ttatcataaa ctatatcgga cagaccatat gcatactgcg
     2281 ccctgatttc ctgagaagcg atgaacagga acgtgaagac taaaactatt tttaaaattt
     2341 tcttgaagct cattgttttt cttactccct ttaaattctc gatttagttg cccggcgtcg
     2401 gggtcggcgt cgccagcggc ggcaaaactt tattggcaag attcagagga attaaaactc
     2461 tggcggcatt cttctgaacc gatgaatcaa aagcggcaaa ttcggcttga ccaagcgatt
     2521 gttttatttt ttctttggca ttcaggatta tttgattttt ttgattttgc agttgtttaa
     2581 gttcagccgg aacgggggga agcgattcaa aggatttttg cggtcgcgcg ggctgctgct
     2641 tattcggagg cgatgacggc tgtatttttt taagctctcc gttcgggaat tgagcgcgat
     2701 acttctttat aatttcgcgg gcgctttggt caatcggctg aatatttgct aaacattcgg
     2761 ttgccgccgt atctacggca gcaaattggg aagactccaa gcctaaccga ttcgtataaa
     2821 aagaattttt gaaattagtc ctttcactct gagccagtga ttcaccgtct ttgtctttta
     2881 acgcctgaat ttcctgaaac aatgcccagt aagccaattc gggcggcatc ggcttggcgt
     2941 ctgtataaag gtttaatctg ggggtcgggg tcggcgacgg cgtttcccct tgtccgctga
     3001 cccgcgtaat tgagttagtt gcggtcacaa taaagcctac tgccataact aaaagc
//
LOCUS       DASWNE010000361         1868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_117422, whole genome shotgun sequence.
ACCESSION   DASWNE010000361 DASWNE010000000
VERSION     DASWNE010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117422"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1736)
                     /locus_tag="VGB68_07300"
     CDS             complement(<1..1736)
                     /locus_tag="VGB68_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049074.1"
                     /translation="MSAFAKNNLSFKLLKKGKIVFFLLLLSLATQVLPNAGVLAQNNA
                     QYEKISSLIDEVPRDLNKRVRQFVEDMRYESAAAHWKDARLDERVIKLYRPDIEGVAY
                     YEFSLVNGRGEKVGYVTLSNGEHEQPIAGWADEGISTVEQLIRQAENNGFIPAKVYKL
                     NELSYVVEDASGRMAAHTPNLPLKIVGQRMEWLDADVQPATIESYTAAAETDENGIPE
                     QQIRRSGDTESHFSLEPWSSWEELKTGYTETYAVMNEWSRRAAASDWKVERALAESGE
                     GLFSDEAALFGALSDNVLSVRVVRGNPEILRAEIIRREGISPAVKVLPIAAPRDEMPV
                     ELLVDYGETTDIIKVTILPESYRERVGGAGKLVNAKVLKDSIRTDRANAGQSKWYGFG
                     NGPWGAWYTSWAAHHEDQRWYDQIPPNTPPNTSNCHSGCGATAWAMLFGWGDHRAWEN
                     DPTWWGRFGLYRANGGYGADAHAPAFQDAGVNNMIWEIRNDILTFCGFNQQGATYPAT
                     MWGAAFYLNGRTGATLSTDFNGFGFGLPWLRDIAANSIVNRDVPVIIGTGFFEHYPLA
                     YGYQYRSRKVNLG"
BASE COUNT          402 a          528 c          476 g          462 t
ORIGIN      
        1 ccgagattga ctttccgcga gcggtactga tagccgtaag cgagcgggta atgctcgaaa
       61 aagccggttc cgataattac cgggacatcg cgattgacaa tcgaattggc ggcaatatcg
      121 cgcagccacg gcagaccgaa tccgaaaccg ttaaaatcgg tcgaaagagt agcgccggtg
      181 cgcccgttca gataaaacgc cgcgccccac atcgtcgccg gataggtcgc gccctgctgg
      241 ttgaatccgc agaaagttaa aatatcgttg cgaatttccc aaatcatatt gttgacgccg
      301 gcgtcctgaa aagccggggc gtgagcatcc gcgccgtacc cgccgttagc gcggtacaaa
      361 ccgaaacgtc cccaccaggt cgggtcgttt tcccacgcgc ggtggtcgcc ccagccgaac
      421 agcatcgccc acgccgtcgc gccgcagccc gaatggcagt tcgacgtgtt gggcggggtg
      481 ttcggcggaa tctggtcata ccatctctgg tcttcgtgat gcgccgccca cgaagtgtac
      541 caggctcccc acggaccgtt tccgaaaccg taccatttcg actgcccggc gttggcgcgg
      601 tctgtccgga ttgaatcttt taagactttc gcgttaacca attttcccgc gccgccgact
      661 ctttcgcgat aagattccgg gagaatcgtg acttttatga tgtcggtcgt ctcgccgtaa
      721 tcgacgagca gctcaaccgg catttcatcc cgcggagcgg caatcggaag gactttgact
      781 gccgggctga ttccctctct ccgaataatc tctgctctga ggatttcggg atttccgcgg
      841 acgaccctga cgctcagcac attgtcggaa agagcgccga acaaagcagc ctcgtcggag
      901 aaaagtccct cgccgctttc cgccagcgct ctttcgacct tccagtcaga ggcggcggcg
      961 cggcgcgacc attcgttcat cacggcgtag gtttccgtat agccggtctt taattcctcc
     1021 catgacgacc agggttcgag actgaaatga gattcggtgt ctccgctcct tcttatctgc
     1081 tgttcgggaa tgccgttttc atcggtttcg gcggcggctg tatagctttc gatggtcgcc
     1141 ggctggacat cggcgtcgag ccattccata cgctgcccga cgatttttaa aggcagattg
     1201 ggagtatgcg ccgccatgcg tccggacgcg tcctcgacga catatgataa ttcatttagt
     1261 ttatagacct tagcgggaat gaagccgttg ttttcggctt gacgaatcaa ctgctcgacc
     1321 gtcgagattc cctcgtcagc ccagccggcg atgggctgtt cgtgttcccc gtttgaaagc
     1381 gtcacgtaac cgactttttc tcctctgccg tttacgaggc tgaattcata ataagctacg
     1441 ccttcgatat ccgggcgata caatttaatt actctctcgt ccagacgcgc gtctttccag
     1501 tgcgccgcag ccgattcgta acgcatatct tcgacgaact gtctgacgcg tttattcaaa
     1561 tcgcgcggga cttcatcaat caggctcgat attttttcat actgagcgtt attttgagcg
     1621 agaacgccgg cgttcggcaa aacctgcgtc gccagcgaca gcaatagcag aaaaaaaact
     1681 atcttcccct ttttcagcaa cttaaacgat aaattatttt ttgcaaaagc tgacatagta
     1741 ttctccttag gtgtttttaa tttgtgatat tcggttagaa aatatattca aaaaaaactc
     1801 aacccgcgtg tcgccggcta gaatcgcaag tcttccggga ttactcgaaa aggcttgttt
     1861 aatttttc
//
LOCUS       DASWNE010000362         2738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_295092, whole genome shotgun sequence.
ACCESSION   DASWNE010000362 DASWNE010000000
VERSION     DASWNE010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295092"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="VGB68_07305"
     CDS             complement(<1..716)
                     /locus_tag="VGB68_07305"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEY0049075.1"
                     /translation="MKATFFRAFFTVILFFVCAALLPAQNYEAPEIPKFDFYTRGAYR
                     AEVPRPQTLLRYDVGDFHTTYAQMEKVIETIAKAAPERVRIFDIGETNEHRMMHIVAI
                     SAPENINRLDEIKASNARLTDSRRTSPGDAAAITQNNPLIAWMAYTIHGNESASFEAM
                     MQVVYQLAASNEPATLDILKNTVVLIVAGENPDGHERFVTWYNSVATGNADRAALEHR
                     EPWSITGRYNRFRFDLNRDN"
     gene            766..990
                     /locus_tag="VGB68_07310"
     CDS             766..990
                     /locus_tag="VGB68_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049076.1"
                     /translation="MNNEQPTMNNERFTKEICPRCEASLLKEWRDLTDEQKFLVERLP
                     LNTEFPPAERKKHLYCPRCWHEVPNAETRA"
     gene            1292..>2738
                     /locus_tag="VGB68_07315"
     CDS             1292..>2738
                     /locus_tag="VGB68_07315"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY0049077.1"
                     /translation="MPQWCVSILLSLIVFPAAIFSQTIDPAFAPVLQDFSSSSGSSRA
                     VASLVQPDGKILVGGVFTVASGLPRSGIARFNADGTVDAAFDAGDISTVDTLTHVGDG
                     GTIQAIALQPDGKIIIGGSFRRGNDTTIRAVERLNANGSPDATFQSTLFNAGVFDIVI
                     QPDGKIVVGGQFQVSAVNPSNGQTVNFKNLARLNADGSFDFSFAANGLDRSNAVALLP
                     DGKIVVANDSQTGLNPSVLNLYNPNGATLTELARFSGGGAEELEIQPDGKYVVAGSFT
                     HVNGIFQRRIARINPDGSLDTTFNTGDGFTNGLYVDDVAIAPDGKIYIVGDFQIFNDA
                     TRWRVARLNANGSIDTTFNTGTPLAGNVAAVSILPDGRLFIGGAFPMPPAGNFYNNIA
                     RMNTDGTYDTNFNNANVTFEGEGYAIRQQPDGKVLIGGTMTAANGLLADGITRYNGDG
                     TLDLNFFRNPDGGIVFDVERQSDGQILIAEGG"
BASE COUNT          655 a          726 c          747 g          610 t
ORIGIN      
        1 aaattatcgc ggttcaaatc gaaacgaaaa cgattgtagc gtcccgtgat cgaccacggt
       61 tcgcgatgtt cgagcgcggc gcggtcggcg ttgcccgtcg ccaccgagtt gtaccacgtc
      121 acgaatcgct cgtgaccgtc gggattttcg cccgcgacga tcaaaacgac cgtatttttt
      181 aaaatgtcga gcgttgccgg ctcgttcgag gcggcaagct ggtagacgac ctgcatcatc
      241 gcctcgaacg aagccgattc gttgccgtga atcgtgtaag ccatccaggc aatcaaagga
      301 ttattttgcg tgatggcggc ggcgtcgccc ggcgaagttc ggcgcgaatc ggtcaagcgc
      361 gcgttgctcg ctttgatttc gtcgaggcga ttgatgtttt ccggcgcgct gatggcgacg
      421 atgtgcatca tccggtgctc gttcgtctcg ccgatgtcaa agattcgcac gcgttcgggc
      481 gcggctttgg caatcgtttc gataaccttt tccatctggg cgtaagtcgt gtgaaaatcg
      541 ccgacgtcgt agcgcagcaa tgtctgcggg cgcggaactt ccgcacgata cgcgccgcgc
      601 gtgtaaaaat cgaatttcgg aatttccggg gcttcgtaat tttgcgccgg aagaagcgcg
      661 gcgcaaacga aaaataaaat gacggtaaaa aacgcacgaa aaaatgtcgc cttcattaaa
      721 aaatcctttg attgaatttg ggaaagactc gtcagaaatt ctacaatgaa caatgaacaa
      781 ccaacaatga acaacgagcg atttacaaaa gaaatttgcc cgcgctgcga agcctctctg
      841 ctcaaagaat ggcgggattt gaccgacgag cagaaatttc tggtcgaacg cctgccgctg
      901 aacacggaat ttccgcccgc cgaaagaaaa aaacatctct attgcccgcg ctgctggcac
      961 gaagttccga acgcggaaac gcgggcgtaa aaccagttat gagttacgat tgaaggaaaa
     1021 cttcttctaa ttcgtaattc ataactgatt aaatcgtttc cgccttcgtt aaacggttcg
     1081 gaacaggcgg gaaaacgtaa aaaagcccgc cgacaaaaat attgttaagc ccgtatttcc
     1141 cgataattca ataataggac gaaagaaaat agaaaaaatc tggttaattt gtatttttat
     1201 tgataaaatc cgcactgtgc gggtgtaata agacttttct caaggagggc tcccaaacgt
     1261 gtctgctcaa acttcttccg cccaccgcgg aatgccgcag tggtgtgttt ccatccttct
     1321 aagtttaatc gtttttcccg ccgccatttt ttcgcaaacc atcgacccgg cttttgctcc
     1381 tgttttgcag gatttcagct cgtcgtccgg ttcttcgaga gcggtggcgt cgctcgtgca
     1441 gccggacggg aaaatcctgg tcggcggagt ttttacggtg gcgagcggat tgccgcgcag
     1501 cggaatcgcg cgctttaatg ccgacggcac ggttgacgcg gcgtttgacg cgggcgatat
     1561 cagcacggtc gatactctga cgcacgtcgg cgacggcggc acgattcagg cgattgcgct
     1621 tcagccggac ggaaaaatca taatcggcgg cagtttccgg cgcggcaacg atacgacaat
     1681 tcgcgccgtc gagcgcttaa acgccaacgg ctcgccggac gcgacatttc aatcaacgct
     1741 ttttaacgcc ggagtttttg acatcgtcat acagccggac gggaaaatcg tcgtcggcgg
     1801 gcagtttcag gtttcagccg tcaatccgtc aaacgggcag acggtaaatt tcaagaatct
     1861 ggcgcggctc aacgcggacg gctctttcga tttcagcttc gcggcgaacg gcttggacag
     1921 aagcaacgcc gtcgctctgc tgcccgacgg taaaatcgtc gtcgccaacg attcgcaaac
     1981 cggcttaaat ccgagcgtgt tgaatctgta taacccgaac ggcgcgactc tcacggaact
     2041 ggcgagattt tccggcggcg gcgcggaaga actcgaaatt caaccggacg gaaaatacgt
     2101 cgtcgccggt tctttcacgc acgtcaacgg aattttccag cgccgcattg ccagaataaa
     2161 cccggacggc tcgctcgata cgacctttaa cacgggcgac ggatttacca acggacttta
     2221 cgtcgacgac gtggcaatcg cgccggacgg caaaatttac atcgtcggcg attttcagat
     2281 ttttaacgat gcgacgcgct ggcgcgtggc gcgtcttaat gcgaacggct cgattgatac
     2341 gacttttaac accggcacgc cgcttgccgg aaacgttgcc gccgtctcga ttttgccgga
     2401 cggcagactt tttatcggcg gcgctttccc gatgccgccg gcaggaaatt tttacaataa
     2461 tatcgcccgt atgaatacgg acggcacgta tgacacgaat ttcaacaacg ccaacgtgac
     2521 ttttgaaggc gaaggctacg cgattcggca gcagccggac ggcaaagttt taatcggcgg
     2581 cacaatgacg gcggcgaatg ggctgctcgc cgacggcatc acgcgctaca acggcgacgg
     2641 cacgctcgac ttgaatttct tcagaaatcc ggacggcgga atcgtctttg atgtcgagcg
     2701 gcagtcggac ggacaaatct tgatcgccga aggcggcg
//
LOCUS       DASWNE010000363         2255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_470655, whole genome shotgun sequence.
ACCESSION   DASWNE010000363 DASWNE010000000
VERSION     DASWNE010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470655"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..1539
                     /locus_tag="VGB68_07320"
     CDS             235..1539
                     /locus_tag="VGB68_07320"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY0049078.1"
                     /translation="MNDPKDPKNLPPDDFSKTTPYIRQPKEELSSAAPKDDYTSDWEK
                     TNFNFSPPPRSSSTPEDDWGKTAVNINLPRSSSSSSSNNQQEDFGKTFMPGQMPQQPQ
                     QPSSGWDMTQANINLGGQENFGGAPRQSDFGATTPYINLPQADRAKYEKTVQPAQTAP
                     QPEEKSGAKKAATPAWVWFGGVALLFFLLAATIIGAYYVFSKKYGYDVLVKGAQPQSE
                     VFVDGTRWGYTSGTGDILVSGLTAGTHKIEIRHPNFTYETEQTNGEDGGKTVEIIAKS
                     RQKQVEPPKDDCANIKKGDFAKSEKCANDALDKLGDNFTVDELLRAMNLYIINFASGK
                     FDIPPTNQKFLEKSAGYMKKLPPNVVIEIGGHTDNTGDDVKNQKLSENRANAVKDALI
                     KFGVNADILQMKGYGKSKPKASNDTEDGKFQNRRIEYIALSK"
     gene            complement(1556..2185)
                     /locus_tag="VGB68_07325"
     CDS             complement(1556..2185)
                     /locus_tag="VGB68_07325"
                     /inference="COORDINATES: protein motif:HMM:NF019559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HEY0049079.1"
                     /translation="MKILSLLAFISAVLTIYFDYQDKRFVYLFKPATMIFIIALAWFC
                     GERKGFYRRAILAGFVFSLVGDTFLINAQNFVFGLASFLVAHFCYVAAFYKAGAGKLK
                     PVSLSAYPVGLAIFMLVSGGVPENLKIAVGVYALAISTMLGAALNFYLTQKTPTALFA
                     LAGAAFFVLSDALLAYNKFNEEIFLAKLLILSTYFFAQWLIAKSLTSQI"
BASE COUNT          672 a          571 c          570 g          442 t
ORIGIN      
        1 gaaggcatca acgtccgaac caaaaacgcg cacccgccga ctgtgaaata accggtgact
       61 gataacggaa agtggaaaat caaggaatgt atcctcgttt tccgttttcc gttttccgtt
      121 ttccattctt cttgacatca acccgcgcgt gttttagctt tgaagcacag caaaaggctt
      181 gcgttttttc cgtcaagttc aagctgattg ccgctgctat taaggagacg caccgtgaac
      241 gacccgaaag acccgaagaa tctgccgccc gacgattttt ccaaaacgac gccgtatatc
      301 cggcagccga aagaggaatt gtcctctgcc gcgccgaaag acgattacac gagcgactgg
      361 gaaaagacca attttaattt ctcgccgccg ccgcgttcgt cgtcgacgcc cgaagacgat
      421 tggggaaaaa cggcggtcaa tatcaatctt ccgcgcagca gcagcagtag cagcagtaat
      481 aatcagcagg aagatttcgg caaaactttt atgcccggtc agatgccgca gcagccgcag
      541 cagccttctt ccggctggga tatgacgcag gcaaacatca acctcggcgg gcaggaaaat
      601 ttcggcggcg cgccgagaca atccgatttc ggcgcgacca cgccgtatat taatctgccg
      661 caagccgacc gcgctaaata tgaaaaaacc gttcagccgg ctcagacagc gccgcagccg
      721 gaagaaaaat ccggcgcgaa aaaagccgcg actccggctt gggtctggtt cggcggcgtg
      781 gcgttgttgt tttttctgct ggcggcgacc attatcggcg cgtattatgt tttctcgaaa
      841 aaatacggct acgacgttct ggtcaagggc gcgcagccgc agagcgaagt tttcgtcgac
      901 ggaacgcgct ggggttatac ttcgggaacg ggcgatattc tggtgtcggg tttgaccgcc
      961 ggaacgcaca aaatcgaaat caggcatccg aattttacat acgaaaccga acagaccaac
     1021 ggcgaggacg gcggaaaaac cgtcgaaatc attgccaaaa gcaggcagaa acaagtcgag
     1081 ccgccgaaag acgactgcgc caacatcaaa aaaggcgatt ttgccaaatc cgaaaaatgc
     1141 gccaacgacg cgctcgataa gctcggcgac aattttacgg tcgacgaatt gctgcgcgcg
     1201 atgaatctgt atattatcaa tttcgccagc ggaaaattcg acattccgcc cactaaccag
     1261 aagtttctgg aaaaatcggc tggctatatg aaaaagcttc cgccgaacgt cgtcatcgaa
     1321 atcggcggac acaccgataa tacgggcgat gacgtcaaaa accagaagct ttcggaaaat
     1381 cgcgccaacg ccgtcaagga cgcgctgatt aaattcggcg tgaatgccga tattttgcag
     1441 atgaaaggct acggcaaatc gaaaccgaag gcttcgaacg acacggaaga cggcaagttt
     1501 cagaatcgaa gaattgaata catagcttta tcgaagtgat atctgatatc caaatttata
     1561 tttgcgaagt cagtgatttg gcaatcagcc attgcgcgaa aaaatatgta gaaagaatca
     1621 ataatttagc gagaaaaatt tcctcgttaa atttgttgta agccagaagc gcatcggaca
     1681 aaacaaaaaa cgccgcgccg gcaagcgcga aaagcgcggt cggcgttttt tgtgtcaggt
     1741 aaaaatttaa agccgcgccg agcatcgtcg aaatcgccag cgcgtagacg ccgaccgcaa
     1801 tcttcaaatt ttcgggaacg ccgcccgaaa ccagcataaa aatcgccaat ccgaccggat
     1861 aagccgaaag cgagaccggc ttgagttttc ccgcgcccgc tttgtaaaac gccgcgacgt
     1921 agcaaaaatg cgcgacgaga aagctcgcca gcccgaaaac gaaattttgc gcgtttatca
     1981 aaaacgtgtc gccgacgagc gagaaaacaa aacccgccag aatcgcccgg cgataaaaac
     2041 cttttctctc gccgcagaac caagccagag cgatgatgaa aatcatcgtc gccggtttga
     2101 aaagatagac gaatcgcttg tcctgataat caaaataaat cgtcagaacg gcggaaatga
     2161 aagccaggag cgaaagaatt ttcatcgttc aaaacagccg cgcataagca cggaaaaaga
     2221 tattgacagg gatgaagggg atgaacaggg atttt
//
LOCUS       DASWNE010000364         3568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_260065, whole genome shotgun sequence.
ACCESSION   DASWNE010000364 DASWNE010000000
VERSION     DASWNE010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260065"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..2513)
                     /locus_tag="VGB68_07330"
     CDS             complement(237..2513)
                     /locus_tag="VGB68_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049080.1"
                     /translation="MRRDSNLNYFRKLTSFVLFFSILSGTFSIGKAQTKLRTNEKPAN
                     AETSRDKVYAELFRRTRGAEFAQLLRKNVGAGEVESIENWLDEGNAGFFRVFDSAYRT
                     ETWRVFEEKRKKNMPGASIVSPPTVPKATSVPKSTVPPKSAVPTKRKTVSLRGAAFAP
                     SMDSVFFRNASYNSLPADSFARTLGDSFEQTSEQDEPTITKIETDDGFQMTGVVAEST
                     KIDGDLTVTRSEKANARAIMVEGGTVGDETTSVQFVEAVNKKERRSLRKETTISWRVL
                     TASCPDADGISAGSATITNSVKTTITNPQTIAVLTRDITVRINSKGFVDDAAELTHFD
                     MEGAAVETISGYDRAERLGLTENSQFADGTRRLNYQIQNNKPGTTVKNEYGYDKKIDG
                     KLGEVKVTAAPGLSTADIRRIEEIAGFNVGWLYTQVDNYLEIARLSWRSGGCVEVTLK
                     TPKMKLLPAESAEVSAETVHKFDKNKVNAELTLQTATESALPDKYTAVTQGTFTLTAP
                     RKGEKAFISVNSVSRRGINFEILQFDEEKVKKNPVKPTKTPPIKKCGGAWSGKITAVK
                     RKREEEVKPASGRLVRQVNSDDETFSIDYHLLGIQDTSKNLANGYFAEAKMNYRSVTY
                     TESNYAPGEMMCDKRIITTPQTLKMEIVRTAASDKRLTVYVSVGGEKGYLTFGSPEVE
                     AEQTVTRTYETKCPSYDQTNSGVDRNDRLIKIVNPSFEIKFELDAKSAYRLVGSKTIQ
                     NSDGSETIVTWNLTRDCK"
     gene            complement(2785..>3568)
                     /locus_tag="VGB68_07335"
     CDS             complement(2785..>3568)
                     /locus_tag="VGB68_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049081.1"
                     /translation="TPCSIGVDTIFSRFFNRSGDAFREITYKNMQEFNSKIKARKGTK
                     FYGLVGHGYNKTCHLTTPGDGLVPAGSALWLIKNFKYTPATSTHEFLLGEDQNMKVIH
                     QWLAISPKGNHKPEVEDYTGSLNTEKSEENFARRRNYGAMFHPVSFATEAAQKDDEDI
                     EPTFVTGVKLDPNQTMEIEIPVPDGKRIALNMLAPKKISATLIDPNATIAGTNLAGTP
                     EAENTFRLISVEKPFQKSKWKLRLESRETETAEIAVVVFVFQ"
BASE COUNT          717 a          919 c          898 g         1034 t
ORIGIN      
        1 aacaaaatac acagtaattt ttgattttcc atcgcctgcg cttcccgtca atcgttttcg
       61 cgccgaaatt tcggcttcaa actttagtgc gcgaaaaggg gcgcaaaagg ttcagaccgc
      121 aaaataaaaa atgcggcgca gaacaattgc gccgcattta aagttcaact gaaatttctt
      181 taacttcagt caaaaaagaa tattcaacgg ctaaaaaaag acccgcttaa caccgcttat
      241 ttgcagtcgc gcgtcaggtt ccacgtcacg attgtttcgc tcccgtcgct gttttggatg
      301 gttttgctgc cgacgagccg gtacgcgctt ttcgcgtcga gttcaaactt aatctcgaag
      361 ctcggattca ctattttgat taagcggtcg ttgcgatcga cgcccgaatt cgtttggtca
      421 tacgaggggc atttcgtttc gtacgttcgg gtgacggttt gttcggcttc gacttccggt
      481 gagccgaagg tcaggtatcc tttttcgccg ccgacggaga cgtaaaccgt cagccgcttg
      541 tccgacgccg ccgtcctcac gatttccatt tttagggttt ggggcgtcgt aatgattctt
      601 ttatcgcaca tcatttcgcc cggcgcgtaa ttgctttcgg tatacgtgac cgagcggtaa
      661 ttcattttcg cctcggcgaa atagccgttc gctaaatttt tgctcgtgtc ctgaatgccc
      721 aaaagatggt aatcgatgct gaacgtttcg tcgtcgctgt ttacttgcct gaccaatctg
      781 cccgaagccg gtttgacttc ttcttcgcgc tttcttttga ccgcagtgat cttgccgctc
      841 caggcgccgc cgcatttttt gatgggcggc gttttcgtcg gtttgaccgg atttttcttc
      901 actttttcct cgtcgaactg caatatttca aaattaatgc cgcgccgcga aacggagttg
      961 accgagataa acgctttttc gcctttccgg ggcgcggtca gagtaaatgt tccttgcgtg
     1021 accgccgtat atttatcggg cagggcggac tcggtcgcgg tttgcaaagt taattcggca
     1081 ttgactttgt ttttgtcgaa cttatgaacc gtttcggcag aaacttccgc gctttcagcc
     1141 gggagcagct tcattttcgg cgtcttgaga gtcacttcga cgcatccgcc cgagcgccag
     1201 ctcaaccgtg ctatttcgag atagttatcg acctgcgtat agagccagcc gacgttgaag
     1261 cccgcgattt cttcgatgcg ccgtatatcg gctgtgctta aacccggcgc agccgtaacc
     1321 tttacttcgc ccagcttgcc gtcgattttt ttgtcgtagc cgtattcgtt tttgacggtc
     1381 gttccgggct tgttgttttg tatctgataa ttgagccgcc tggtcccgtc ggcgaattga
     1441 gaattttccg tcaagccgag ccgctcggcg cggtcataac cggaaatcgt ttccactgcc
     1501 gcgccttcca tatcgaaatg cgtcagttcg gcggcatcat cgacgaaacc tttagagttt
     1561 atccgcacgg tgatgtcgcg cgttaagacg gcgatggtct gcgggttggt gatcgtcgtt
     1621 ttaacggaat tcgtgatcgt cgcgcttccg gcggaaatgc cgtccgcatc gggacatgaa
     1681 gccgtcagaa cacgccagct gatggtcgtt tcttttcgca ggctccggcg ctcttttttg
     1741 ttgaccgcct cgacaaactg gacgctcgtg gtttcgtctc cgacggttcc gccctcgacc
     1801 ataatcgcgc gcgcgttcgc cttttcgctc ctggtgacgg tcaggtcgcc gtcgattttc
     1861 gtgctttccg ccacgacgcc ggtcatttga aatccgtcgt cggtttcgat tttcgtgatc
     1921 gtcggttcgt cctgttcgct cgtttgttca aacgaatcac cgagcgttcg agcgaacgaa
     1981 tcggcgggca ggctgttata agaagcgttc cggaaaaaga cgctgtccat tgaaggagcg
     2041 aaagccgcgc cgcgcaggct gacggttttg cgtttcgtcg gcacagcgga tttcggggga
     2101 acggtagatt tcgggacgct agtcgctttc ggtacggtcg gcggcgaaac aatcgacgcg
     2161 cccggcatgt tcttttttct tttttcctcg aaaacgcgcc aggtttccgt tcggtaagcc
     2221 gagtcgaaga cgcgaaaaaa accggcgttg ccttcgtcca gccagttttc gatgctttcg
     2281 acttcgcctg cgccgacatt tttccttaat agttgggcga attcagctcc gcgcgtgcga
     2341 cgaaacagct cggcgtagac tttgtctctg cttgtttccg cgttcgcagg cttttcgttc
     2401 gtccgtaatt tcgtctgcgc tttgccgatt gaaaaagtgc cggacaaaat gctgaagaaa
     2461 agaacaaagg aagttaattt ccgaaagtaa tttaaatttg aatcgcgtcg catatttttc
     2521 tcctctttga aatcccgcgc ggcgggtttt tctgtcgatc attagtgcgc aaacgagcga
     2581 aaaaagtatc agtcaaaaaa gcggaaattc aattttgaga taaaaaaagc ggcgagagtt
     2641 cgactcgccg cttctgcgcg atttaatttg gtcgtcactc ggctcgaaac gcaaccggct
     2701 cttttaatgc ggtctcaaac gaatcgccgg cgtgtttctt cccgagtttt cgggcgcgaa
     2761 tttccattaa ccgatttcta ctatttattg gaagacaaaa acaacgaccg caatctcggc
     2821 ggtttcggtt tcccggcttt caagcctcaa tttccactta cttttttgaa acggtttttc
     2881 cacactaatc aaacggaacg tgttttcggc ttcgggcgtt cccgccaaat tcgtgccggc
     2941 gatcgtcgcg ttcggatcaa tcaaggttgc tgatattttc ttcggggcaa gcatattgag
     3001 agctattctt ttgccgtccg gaaccggaat ttcgatttcc attgtctgat tcggatcaag
     3061 cttgacgcct gtgacgaagg tcggttcgat gtcttcatcg tctttttgcg cagcttccgt
     3121 cgcaaaactt acgggatgaa acattgctcc gtaatttctt cgccgggcga aattctcttc
     3181 ggacttttca gtattcaaac ttccggtata atcttcgact tcgggtttat gattgccctt
     3241 cgggctgatg gcgagccatt gatgaatgac cttcatattt tgatcttcgc cgagcaggaa
     3301 ttcgtgggtt gacgtcgcgg gcgtatattt gaaattttta atcagccaga gcgccgaacc
     3361 cgccggaacc agaccgtctc cgggcgtcgt caaatggcag gttttgttgt atccgtgccc
     3421 gaccaagccg taaaatttcg tgcctttgcg agcctttatt ttactgttaa attcctgcat
     3481 atttttgtat gtgatttcgc ggaaggcgtc gccgctgcga ttgaaaaacc ggctgaaaat
     3541 cgtatctact ccgatcgaac acggcgtt
//
LOCUS       DASWNE010000365         2381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_647395, whole genome shotgun sequence.
ACCESSION   DASWNE010000365 DASWNE010000000
VERSION     DASWNE010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647395"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VGB68_07340"
     CDS             complement(<1..393)
                     /locus_tag="VGB68_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049082.1"
                     /translation="MTAKEHNKLVGIFLMVNGGLQVFSGIIVGLIYGGMGAAFLSTAR
                     RDNEQFLGSFMIIMAIIIVPIVLAFGGLFLLAGWKMLKEKAGARNWGIAASIVALLGF
                     PLGTVLGIYGIWFLFGEEGKRFYLGGASS"
     gene            complement(586..1779)
                     /locus_tag="VGB68_07345"
     CDS             complement(586..1779)
                     /locus_tag="VGB68_07345"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY0049083.1"
                     /translation="MSDTKDAVIISAVRTGTGKFQGSLKSFSATDLGAIVVREAVKRA
                     GVNPADVNEVIMGCVVQAGLGQAPARQAALKADLPPEVSAMTVNMVCGSGLRAVSLAA
                     QAIKLGDADFVVAGGMESMSNIPYALPNARDGYRMGNGQMLDLLIHDGLWCPFENWHM
                     GNTGEVVSEKYQITREQQDDYAYNSHRKAVEARLAGRFKDEIVPIEIPQKKGEPILFD
                     HDETVREDTNVESLGKLKPAFKREGGTVTAGNAPGVNDGASAVVVTSAAKAAELGIEP
                     LARIAGYATSGIEPKMIMMAPVQGVLNVLEKTGWKMEDVDLFELNEAFSVQALGVMKE
                     LGLFMDRVNVNGGAVALGHAIGNSGGRVLTTLLYEMKRRDAKRGIAALCLGGGNSVAM
                     AVERD"
     gene            complement(2134..>2381)
                     /locus_tag="VGB68_07350"
     CDS             complement(2134..>2381)
                     /locus_tag="VGB68_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049084.1"
                     /translation="KIKISRDIRDVIAGLEQQLTAAMTERDTVDAENLKLEQNVAKAI
                     AGDPNFGDDSALYEGTGRVRKSERKSGLTRKKKADET"
BASE COUNT          528 a          739 c          547 g          567 t
ORIGIN      
        1 tgatgatgcg ccgcccagat aaaatctttt gccttcttcg ccaaacaaaa accagattcc
       61 gtaaatgccg agcaccgttc cgagcggaaa accgagcagc gcgacgatgc tggcggcgat
      121 gccccagttt cgcgcgccgg ctttttcctt taacatcttc cagcccgcca gcagaaacaa
      181 tccgccgaaa gccagcacaa tcggcacgat gataattgcc attattatca taaagctgcc
      241 gagaaactgt tcgttgtcgc gccgcgccgt gctcagaaaa gccgcgccca ttccgccgta
      301 aatcagcccg acgattattc cgctgaagac ctgcaaaccg ccgttcacca tcagaaaaat
      361 gcccacgagc ttgttatgtt cttttgccgt catcgttttc caacttcctt taaaaattta
      421 ccgcctgacc ggtgtttgaa tcttacttta acttacgtca atcgacaacg aaagttgcga
      481 aaatcaatta cgaattacga attagaaatt acgaatggtc gatttaactc ggcggcttga
      541 gttaatcgac catttgtaat tcgtaatttt taattcgtaa ttgaattaat ctctctcgac
      601 cgccatcgcc accgaatttc cgccgccgag acacagggcg gcgattccgc gtttggcgtc
      661 gcgccgcttc atttcataaa gcagcgtggt caaaacgcga ccgcccgaat ttccgatggc
      721 gtgtccgagc gcgaccgcgc cgccgttgac gttgaccctg tccataaaca gaccgagctc
      781 cttcatgacg ccgagcgcct gcacggaaaa cgcttcgttc agctcgaaca aatcgacgtc
      841 ttccattttc cagcccgttt tttccaaaac gttcagaacg ccctgcaccg gagccatcat
      901 aatcattttc ggctcgatgc ccgaagtcgc gtaaccggcg atgcgcgcca gcggctcgat
      961 gcccaattcc gccgccttcg ccgccgacgt gacgaccacc gccgacgcgc cgtcgttgac
     1021 gcccggcgcg ttgcccgccg tcacggttcc gccttctctt ttgaacgcgg gtttcaattt
     1081 gcccagactt tcgacgtttg tgtcttcgcg cacggtctcg tcgtggtcaa acaaaatcgg
     1141 ctcgcccttt ttctgcggaa tctcgatggg cacaatctcg tctttgaatc tgcccgccag
     1201 ccgtgcttcg accgccttgc gatgcgagtt gtaggcgtaa tcgtcctgct gctcgcgcgt
     1261 aatctggtat ttttcagaaa cgacctcgcc cgtgttgccc atatgccagt tttcgaacgg
     1321 gcaccacaga ccgtcgtgaa tcagcaaatc gagcatctgc ccgttgccca tccggtagcc
     1381 gtcgcgcgcg ttcggcagag catacggaat attcgacatc gactccattc cgcccgccac
     1441 cacgaaatcg gcgtcgccca gcttaatcgc ctgcgccgcc agcgaaaccg cccgcaaacc
     1501 cgagccgcaa accatattga ccgtcatcgc cgaaacttcc ggcggcaaat ccgccttcag
     1561 agccgcctgc cgcgccggag cctgtccgag ccccgcctgc acgacgcagc ccataatcac
     1621 ctcgttaacg tcggcgggat taacgcccgc gcgcttgacc gcttcgcgca cgacgatagc
     1681 gcccaaatcc gtcgccgaaa acgactttaa agaaccctga aacttgcccg tgcccgtccg
     1741 cacggcgctg ataattaccg catctttagt atctgacatt taatcaaatt tcccttttat
     1801 tattgatata aaaactattg tttagatgat aacaaatgag ccggagttta tcacttaatg
     1861 aacggtaaac ggctctgaaa gcggtaaacg gtaaacggta aacggtaagt tgtaagcggt
     1921 aagttatgaa tcccgaatcc cgaatcccga atcccgaatc ccgaatcctg aatcctgaat
     1981 cctgaatcct gaatcctgaa tcctgaatcc tgaatcctga atcctgaatc ccgaatcccg
     2041 aatttcgagc gataaacgaa aaacggcaag cggaagcaac gagcttaccg tttaccgctt
     2101 accgtttacc gttttttacc gcttaccgtt tttttaagtt tcgtcggctt tctttttccg
     2161 cgtcaatccg ctcttgcgct cggatttgcg gacgcgtccc gtgccttcat agagtgcgct
     2221 gtcgtcgccg aaattcgggt cgccggcgat ggctttggcg acgttctgtt ccagcttcag
     2281 gttctccgca tcgaccgtgt cgcgctcggt catcgcggcg gtcaactgct gttccagccc
     2341 ggcaatcacg tcgcggatgt cgcgcgaaat cttgattttc g
//
LOCUS       DASWNE010000366         2482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_364162, whole genome shotgun sequence.
ACCESSION   DASWNE010000366 DASWNE010000000
VERSION     DASWNE010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364162"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VGB68_07355"
     CDS             <1..552
                     /locus_tag="VGB68_07355"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY0049085.1"
                     /translation="EPLWRGAPADYQIELIPREQIQILYPAPYAPALLQYAPERGLDA
                     RFLLSIMRQESRYRADVKSYAAARGLMQFISDTSSRIAVELGRKNFQQDELYNPPIAV
                     LFGAQYLQNLFRMFPNQPQAVAASYNGGETNMQRWLARAKSDQPDRYVPEILFSQSKD
                     YVYRVMANYRVYQLIYDEKLKAR"
     gene            710..1597
                     /locus_tag="VGB68_07360"
     CDS             710..1597
                     /locus_tag="VGB68_07360"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY0049086.1"
                     /translation="MRYLLFILFSILLFAACQKSQTQTQNAPADVKRYNMKGKVVSVD
                     KAKKKAEIAHDDIPGYMDAMTMSFPIRADWVWEDLAPGAEIRAALVVDDAKGDYWLEN
                     IGIIAAPNPNQPTPPVDERFAQIGREIPAFTLTNQDGKRFSIKDYRGTALAITFIYAQ
                     CPLPDYCIKMSTSFSDLANQVRDTDLKDKIRLLSISFDPARDTPEKLKQYGRGYLGKD
                     APPDFAVWQLAVGSDKEVREVADFFGLRYEVDQNDKTQFSHSLRTAVVAPDGKVTKIF
                     AGNEWTPNDLLREMQATLK"
     gene            complement(1820..>2482)
                     /locus_tag="VGB68_07365"
     CDS             complement(1820..>2482)
                     /locus_tag="VGB68_07365"
                     /inference="COORDINATES: protein motif:HMM:NF025701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HEY0049087.1"
                     /translation="AKTYAHLQTAVENLPDASTIDFTKESVVAAFAGTKPSAGWEVLI
                     RRLSDKVLIDLNEPRKDMMHAQMLTAPYKIAVVPVEEEKALALDATPTWTNKMASYRV
                     SKGSFIYSGGFAFRERTFGAEGTIGVLTYGDLLTMTFNLTAKGDKSMMLAEIVSGSLK
                     DGKIELARLDAGTFSENPKPPVNVSGTISDTKLSLRFEPNPTNVADGFQARGTIEAVK
                     VK"
BASE COUNT          584 a          659 c          649 g          590 t
ORIGIN      
        1 gaaccgctct ggcgcggcgc gcccgccgat tatcaaatcg aattgattcc gcgcgagcaa
       61 atccagatac tttatcccgc gccatacgcg ccggcgcttt tgcaatacgc gccggaacgc
      121 gggctggacg cgcgctttct gctttcgata atgcggcagg aatcgcgtta tcgagccgac
      181 gtcaaatcct acgcggcggc gcgcggtctg atgcagttta tctccgatac ttcgagccgg
      241 attgccgtcg agctgggcag aaagaatttc cagcaggacg agctttacaa tccgccaatc
      301 gccgttttgt tcggcgcgca gtatctgcaa aatcttttcc gtatgtttcc gaaccagccg
      361 caggcggtcg cggcaagcta taacggcggc gaaacgaata tgcagcgctg gctggcgcgc
      421 gccaaatccg accagcccga ccgctacgtg ccggaaattc tcttttcgca atcgaaagat
      481 tacgtctacc gcgtgatggc gaattaccgc gtttaccagt tgatttacga cgaaaagctg
      541 aaagcccgct agagattaac cggataattt taaacgccgg aacgcgaaac gatgaatggc
      601 gaattcttta ttccgcgcac ggcgttcgaa gcgccgggca tttcttttta atttgtcttt
      661 gtttttcgat tcgtgcagaa ttatttcaaa caattccagg ggaaatctta tgcgctattt
      721 attattcatt ttattttcga ttttactttt cgcggcgtgt caaaaatcgc agacgcagac
      781 gcaaaacgct ccggctgacg tcaagcgcta caatatgaaa ggcaaagtcg tatcggtcga
      841 caaggcgaaa aagaaagcgg aaatcgcgca cgacgacatt cccggctata tggacgcgat
      901 gacgatgagc tttcctatcc gcgccgactg ggtctgggaa gatttggcgc cgggcgcgga
      961 gattcgcgcc gctctggtgg tcgacgatgc caaaggcgat tactggctgg aaaacatcgg
     1021 catcatcgcc gcgccgaacc cgaatcagcc gacgccgccc gtcgatgagc gcttcgccca
     1081 aatcggcagg gaaattcccg ctttcacatt gaccaatcag gacggcaagc gtttttcgat
     1141 aaaagattat cgcggcacgg cgctggcaat cacgtttatc tacgcgcaat gcccgctgcc
     1201 cgattactgc atcaagatgt cgacgagttt cagcgacctc gccaaccagg tgcgcgatac
     1261 ggatttgaaa gacaagattc gccttttgag catctcgttc gacccggcgc gcgacacgcc
     1321 cgaaaagctg aaacaatacg ggcgcggcta tctcggcaaa gacgcgccgc ccgatttcgc
     1381 cgtctggcag ctcgccgtcg gctcggacaa ggaagtgcgc gaagtcgccg attttttcgg
     1441 tctgcgctac gaagtcgacc agaacgacaa aacccagttc agccattcgc tgcgcacggc
     1501 ggtcgtcgcg cccgacggca aagtgacgaa aatcttcgcg ggcaacgaat ggacgccgaa
     1561 cgatttgctg agagaaatgc aggcgacgct gaaataaaat tttcggctct tgtataatat
     1621 ttaattaaat taacttttcg tagcgtcaca aaaaatattt caggcgcaag aaacatagaa
     1681 tttttcttgc gcctgatttc ataatagttt ataaaacatc atattatatt cacttagtcg
     1741 ggcggcactc tataagcctc agctgacggg cttaagactt aacggcattt gcaaaacaga
     1801 ttgatattag tgctgaattt cactttactt tcacggcttc gattgttcct ctggcttgaa
     1861 agccgtcggc gacgttcgtc gggttcggct cgaatcttaa cgacagcttc gtgtccgaaa
     1921 tcgttcccga aacgttgacg ggcggtttcg ggttttcgga aaaagttccg gcgtcgaggc
     1981 gcgcgagttc gattttcccg tccttcaggc tgccggagac aatttcggcg agcatcattg
     2041 atttatcgcc tttggcggtc aggttaaacg tcattgtgag caaatcgccg taagttaaaa
     2101 ctccgattgt gccttccgcg ccgaaggttc gctcgcggaa agcgaagccg ccggaataga
     2161 taaaacttcc ctttgaaacg cgataggacg ccattttatt cgtccacgtc ggcgtcgcgt
     2221 ccagcgcgag agccttttct tcttcgaccg gaacgacggc gattttataa ggcgcggtca
     2281 acatttgcgc gtgcatcata tctttgcgcg gctcgttcag gtcaatcaaa actttgtcgg
     2341 aaagccgtcg aatcagaact tcccagccgg cactcggttt tgttccggca aaagcggcga
     2401 cgaccgactc tttggtgaaa tctatagtgg aagcgtcggg cagattttcg accgcggttt
     2461 gcagatgcgc gtaggttttc gc
//
LOCUS       DASWNE010000367         2162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_117700, whole genome shotgun sequence.
ACCESSION   DASWNE010000367 DASWNE010000000
VERSION     DASWNE010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117700"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VGB68_07370"
     CDS             complement(<1..405)
                     /locus_tag="VGB68_07370"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEY0049088.1"
                     /translation="MRRISCNLILAVGLILTMGWTAETFAAGSSIDVKRPKNAGILSV
                     QVTFNAPAKPSAYRVLVDGVEVGLAGNSTPAEFYLSPGIHKVEIEGPNNQVFVREVEI
                     RRGVKNCICLRVVERTDKRPCPYDIRVDAPEEV"
     gene            complement(532..1143)
                     /locus_tag="VGB68_07375"
     CDS             complement(532..1143)
                     /locus_tag="VGB68_07375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY0049089.1"
                     /translation="MKNPKLRVLLSLVSLFSMLMAANSAFAAPVRFEQVQQVLNVKPG
                     RANTGGFAQLRFADDGVLKADGDDDDDKTVQPQQDDDRVIVTTTTSIEEENCNCEQPI
                     VRQGFPKWAFLGLAVIPLIFIFRDRDRTKKTPPPQTETPTPTLTPTVTPTMTPKITPT
                     PEPVPEPMTILLFGTGLAGIGLAARRRLRKRNSDGENDEQNES"
     gene            complement(1476..>2162)
                     /locus_tag="VGB68_07380"
     CDS             complement(1476..>2162)
                     /locus_tag="VGB68_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEY0049090.1"
                     /translation="ILSDIAGAEDHYGDMDFKVTGTSEGITALQMDIKVAGINAMILQ
                     EALEQARKGRLHILEIMAQAIEKPREELSEYAPRIITIMIHPDKIRDVIGPGGKIIRS
                     ITEETGAKIDISDDGTVNIATADGEAAQKAVERIRALTAEAEIGETYLGTVTRIVDFG
                     AFVEIFPGLDGLLHISEISDRRIKDVRDELKEGQQVMVKCIGKEGNKVKLSRKAILKE
                     EKSRANGSDA"
BASE COUNT          466 a          590 c          508 g          598 t
ORIGIN      
        1 gacctcttcg ggcgcatcga cgcgaatgtc atacgggcac ggtcttttat cggttctttc
       61 gacaactctc aaacagatgc agttttttac gcctcttctg atttcgactt ccctgacgaa
      121 aacctggtta ttcggacctt cgatttcaac cttatgaatg ccgggcgaaa gatagaattc
      181 cgccggagtc gagttgcctg ccagaccgac ctcgacgccg tcaaccagca cacgataagc
      241 cgacggtttc gccggcgcgt taaacgtcac ctggacggag agaattcccg cgtttttcgg
      301 tcttttcacg tcgatgctgc tgcccgcagc aaaagtttcg gcagtccagc ccattgttaa
      361 aatcagcccg acggcgagta ttaaattgca gctaattctt cgcatctttt cttcctcaat
      421 tcgttgaccg caaaagcgga aattcacgct gcgcgtcaaa tctttataaa aacttttcaa
      481 aatttatatg gaacgctcaa gccgaagctt cggcttgagc gttccgtaat cttaactttc
      541 attttgctcg tcattctcgc cgtcgctatt gcgctttctc aatctgcggc gcgcggcgag
      601 cccgatgccc gcgagtcccg ttccgaaaag caaaatcgtc atcggttccg gcaccggctc
      661 cggcgtcggc gttattttcg gagtcatcgt cggcgtcacc gtcggcgtca aagtcggcgt
      721 cggcgtttcg gtctgcggcg gcggcgtctt tttagttcgg tcgcggtcgc gaaatataaa
      781 tatcagaggg atgaccgcca agcctaaaaa cgcccacttc ggaaaaccct gccgcacgat
      841 cggctgctcg caattacagt tttcttcttc aatagaagtg gtcgtcgtca caatcacgcg
      901 gtcgtcatcc tgctgcggct ggacggtttt atcgtcgtcg tcgtcgccgt cagcctttaa
      961 cactccgtcg tcggcaaaac gcaattgagc gaaaccgccc gtattggctc tgcccggttt
     1021 aacgttcaga acctgttgca cctgctcgaa acgaaccggc gcggcaaaag cggaattggc
     1081 tgccatcagc atcgagaata agctgaccag agacaggaga actctaagtt ttggattttt
     1141 catatcgtct ttcctaacag gaaaccttca tttttcaaca attctagcgc acacaaatcc
     1201 gtctgtggat tcgctgcttt gaaattaagg aaggggcaat gcgggcaaat atagcagaat
     1261 tcttttcaaa atgcacgatt tattttccgg attttcgaat tttttccgcc ttttgccttt
     1321 ctgccgccat ccgattcgga tgttcgttac acaagagtca gtattaatag ataaagtttt
     1381 ccagaattta cacgattttt ttcgctaaaa ccgaaaaagg agaagaaaat atttccttct
     1441 cctttttttt cttcgattta ggttaagttt ccgccttagg catcgctgcc gttggcgcgc
     1501 gatttttctt ctttcagaat cgctttgcgc gacaatttaa ctttgttgcc ttctttgccg
     1561 atgcatttga ccatcacctg ctgaccttct tttaattcgt cgcgaacgtc cttgattcga
     1621 cggtcggaga tttccgaaat gtgcagcagc ccgtccagac ccggaaagat ttcgacaaac
     1681 gcgccgaaat cgacgatgcg cgtcaccgtt cccagataag tttcgccgat ttcggcttcg
     1741 gcggtcagcg cgcgaatgcg ttcgaccgct ttctgcgcgg cttcgccgtc ggcggtggcg
     1801 atgttgaccg ttccgtcgtc ggagatgtcg attttcgcgc cggtttcttc cgtaatcgag
     1861 cgaataatct tgccgcccgg tccaatcacg tcgcggattt tatccgggtg aatcataatc
     1921 gtgataatgc gcggcgcgta ttcggaaagc tcctcgcgcg gcttctcgat tgcctgcgcc
     1981 ataatttcca gaatatgcag ccgacctttc cgagcctgct ccaaagcttc ctgcaaaatc
     2041 atcgcgttga ttccggcgac tttaatatcc atctgcaaag ccgttatgcc ttcagacgtg
     2101 ccggtgactt tgaaatccat atcgccgtaa tggtcttcgg cgccggcgat gtcggataaa
     2161 at
//
LOCUS       DASWNE010000368         6423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_117726, whole genome shotgun sequence.
ACCESSION   DASWNE010000368 DASWNE010000000
VERSION     DASWNE010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6423
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117726"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VGB68_07385"
     CDS             <1..417
                     /locus_tag="VGB68_07385"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY0049091.1"
                     /translation="LIDVLLVLLIIFMVISPSKPSRFEAKVPAEPKPEDQNVVLKPNP
                     NTLIVFIDKEGKLRLNQDEMGNIADSSGLVTKLTEIFNYRDANGIFRENTNETEKTVF
                     IKAPRSVKYGEVAKVVDAAKSAGAQPVGLQVDDLEP"
     gene            580..1512
                     /locus_tag="VGB68_07390"
     CDS             580..1512
                     /locus_tag="VGB68_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049092.1"
                     /translation="MRVSRVGIFALIAITVLTGAGCNFWNQIKARQDLVDGAKAYSDK
                     KFEEAETLFRDAVRRDPSNLLGQLFLARTLHSQYAANRTQTQKADEAVAEYRKVVPEY
                     KQEVARKKQALDANPGDEKAQGEYTNSLTILNSSISAIANLLDTTSKPDEWKAWQTEI
                     ANDAGLPNETRAAALTSMASKENTCANDITGVEPVRKTVKVNGKDEYQYVKPTDPKVY
                     EDLKGCIARGTELVNKALELDKNSDSIWSYKTSLLIQDMRVAEMDGKKEDQARFKEEA
                     DKAKAEFTRLAEIKRRAKEEEEARRKAAEEEAKQ"
     gene            1678..3891
                     /locus_tag="VGB68_07395"
     CDS             1678..3891
                     /locus_tag="VGB68_07395"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEY0049093.1"
                     /translation="MVRIENIIGKVEQNHPQADVELIRRAYLFSALHHRGQKRASGEP
                     YLVHPLAVADLLADMRLDEISVCTGLLHDVVEDTLVDLDTLRTSFGEEITKLVDGLTK
                     IAHISNLSKEEQQAENVRKMVLAMITDVRVVLIKLADRLHNMRTMEFLKPEKRARISQ
                     ETLDIYAPIAHRLGMGKLRSELEDLSFQNLYPEDYRKLSAEVDARRPELEAMLEKITG
                     TIKENLAENDVPFIQVQGRVKRLYSLWKKLKNRKLAIEQVYDLIAARIITENDKKDCY
                     IALSVIHDIWTPVPERFKDWIAIPRDNLYQSLHTSVIGEGGHAFEVQIRTEDMHRIAE
                     EGVAAHWKYKEKKLGKHEDDQNLDELRKTVEKLLLPLVEETSDLNDSQDFIESLKLDL
                     YPKDVYAFTPMGKVIQLPRGATPLDFAYAIHSEVGDTCTGAKIGGRIVPLKTEIQNGD
                     VVEILTSPGSHPSRDWLNYVATSKAKNRVRHWIAEHQRAESIEIGRKLLSTEAEKFRL
                     TAKKVIGDEENLKRIANEYGLGRIEDLLASIGYGKTLPRNVLAKYLGAEKFAELDPEK
                     KKETTFQSGVKAVKKLIGFGEDAIIVKGVDNLLLTRALCCNPLRGEEIVGYISLGKGI
                     VVHNKRCKNVQALMVNKDRIVEVEWAKIDDKQIQAVRLLATTENRTGMLAGITNAIAE
                     IKTGIRDASARVSRDNQGLIEVTIEVFDKKHLDKVTSAIKNVSGVIDVERVNDLA"
     gene            4082..4459
                     /locus_tag="VGB68_07400"
     CDS             4082..4459
                     /locus_tag="VGB68_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY0049094.1"
                     /translation="MKEIISTEKAPGAIGPYSQAVKTGNMVFLSGQIPIDPQTGEFVA
                     GGVAEQTEQVLKNLSAVLEAAGTSLNDVVKTTVFLADMNDFTAMNEVYARYFSENKPA
                     RATVQAARLPRDARVEIECIAII"
     gene            complement(4591..4785)
                     /gene="rpmB"
                     /locus_tag="VGB68_07405"
     CDS             complement(4591..4785)
                     /gene="rpmB"
                     /locus_tag="VGB68_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014398134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEY0049095.1"
                     /translation="MAKRCDVCGKGPQFGNNVSHANNKTRRRFNPNLQSIRVKAPSGA
                     AVRQKVCTRCLKAGKVVKAV"
     gene            4897..6159
                     /locus_tag="VGB68_07410"
     CDS             4897..6159
                     /locus_tag="VGB68_07410"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEY0049096.1"
                     /translation="MVNFEGAKAKVRQFSFRFSAIAVLAIATLAFTACGPTTGTGGGQ
                     TPGGSDTAATVNGKAIKMEQVERVIKAQTQGQDSRLSPLELAQARLQVLDQLIQQEVM
                     YQKADAEKTVPADDKVTEELNKLKQQSGKSAEEFDKQMKDAGESEESLRESIKKNLAI
                     QALNEKVTSKIEPPKDQEIVDFFNGNKEGFKNKRGAQLGVIVIDPRKVDANDTTGNDI
                     EAQQKAKEIGDRLIRGADFATVAREASEDPQTRLQGGDWRYFSEEEMKQAFGGGFAEF
                     VMTKLQNGQIIPQTIPFEGRLLIVKLQRKQEQDEDRTLETPGVRQQITDLLINSRKQL
                     LSQAYMAMAMDGAKVENLLAKQVVENPNNLSGARPASTEPAASPTAAANTNINTNVNV
                     NAAPAKPANANTKPATVPAANTNANARR"
BASE COUNT         1848 a         1515 c         1672 g         1388 t
ORIGIN      
        1 ttgattgacg tgcttcttgt gctgctgatt atttttatgg tgatttcgcc gtcgaaaccg
       61 agccgtttcg aagcaaaggt gcctgccgag ccgaaaccgg aagaccagaa tgtagttctg
      121 aaaccaaacc cgaacacgct gattgtcttt attgataaag aaggtaaatt aagactcaat
      181 caggacgaga tgggtaatat tgcggattcc agcggtttgg taaccaagct gacggagatt
      241 ttcaattatc gtgacgctaa cggtattttc cgtgaaaaca cgaatgaaac cgaaaagaca
      301 gtctttatca aagcgccgcg ttccgtgaaa tacggtgaag tggcaaaagt ggtcgatgcc
      361 gccaagtctg ccggtgcgca gcctgtcggt ttgcaagttg acgatttgga accataaaag
      421 ggatttgcga ttttaaaagg ggtttttggt cttgggtctt cggtctttga tttgataact
      481 ccgcttgagc agagaaaaat tcgaagaccg aaaaccgaag ttcaaagatt gattgataag
      541 gtcgcaaatc taaaattata agataacgga gtcgaaaaaa tgagagtctc tcgagtaggg
      601 atatttgctt taatagcgat aacagtttta accggagccg ggtgcaattt ctggaatcag
      661 atcaaagcgc gtcaggattt ggtcgacggc gcaaaagctt attcggacaa aaagtttgaa
      721 gaagccgaaa cgcttttccg cgatgccgtc aggcgcgacc cgagcaactt gctgggacaa
      781 ttgtttctgg ctcgcacgct tcattcccag tatgccgcca accgaaccca gacgcagaaa
      841 gcggatgagg cggttgccga atacagaaaa gtcgttccgg aatataagca ggaagtggcg
      901 agaaagaaac aggctcttga tgcaaatccg ggcgacgaaa aagcccaagg ggaatacacc
      961 aatagtttaa cgattctgaa cagttcgatc agcgccattg caaatttatt ggacacaacc
     1021 agtaagccgg acgaatggaa agcctggcaa actgagattg ccaatgatgc gggcttgccg
     1081 aatgaaacaa gagcggcggc tctgacctcg atggcgtcaa aagaaaacac ctgcgccaat
     1141 gacattaccg gcgtcgagcc ggtcagaaaa accgtcaagg ttaacggcaa ggacgagtat
     1201 caatatgtta aaccgaccga cccgaaagtt tacgaagacc tgaagggctg tatagctcgc
     1261 ggcacggaac tggttaacaa agctctcgaa ctggataaaa acagcgattc gatatggtct
     1321 tataaaacga gtcttctgat tcaggatatg cgcgtcgcgg aaatggacgg caaaaaagaa
     1381 gatcaagccc gttttaaaga agaagccgac aaagcgaaag ccgagttcac tcgtttagct
     1441 gaaattaaac gccgggcgaa agaagaggaa gaagcgcgca gaaaagccgc agaagaagaa
     1501 gcaaaacaat aatttcttta aaaatcattt tatgaaaagg gtgaggctca attaaaataa
     1561 aattgagcgt cgccttttat tttcagcgat gagggcgaaa tattttgtaa gctgtgggcg
     1621 cgaggctgta tataataact gccaaccgcc gtctgctttc tgccttctta atagtttatg
     1681 gttcgcatcg agaacattat cggcaaagtc gaacagaatc atccgcaggc tgacgttgag
     1741 ctgattcgcc gcgcatatct gttctccgct ctgcatcatc gcgggcaaaa acgcgcttcg
     1801 ggcgagccct atctggttca tccgctggcg gttgccgatt tgctggcgga tatgcggctc
     1861 gatgaaattt ccgtttgcac cggacttttg cacgatgtgg tcgaagatac tctggttgac
     1921 ctcgatacgc ttcgcacctc attcggcgag gaaatcacca aactggttga cgggctgacg
     1981 aaaatcgcgc atatcagcaa tctttccaaa gaagaacaac aagccgaaaa tgttcgcaag
     2041 atggttttgg cgatgataac ggacgtccgc gtcgtgctta ttaaactcgc cgaccgcctg
     2101 cataatatgc gcacgatgga atttctgaaa cccgagaaac gggcgcgtat ttcgcaggaa
     2161 acgctggata tttacgcgcc gatcgcgcac cggctcggaa tgggtaaact ccgcagcgag
     2221 ctcgaagatt tatcgtttca gaatctttac ccggaagatt accgcaagct ttccgccgaa
     2281 gtcgatgccc gccgcccgga gctcgaagcg atgctcgaaa aaattaccgg gacaattaaa
     2341 gaaaatctgg ctgaaaacga tgtgccgttt atacaggtgc agggtcgcgt caagcgtctc
     2401 tattcgctct ggaaaaaact gaaaaaccgc aagctcgcca tcgagcaggt ttatgattta
     2461 atcgccgccc gcatcatcac cgaaaacgat aaaaaggatt gttatattgc tttaagcgtg
     2521 attcacgaca tctggacgcc ggttcccgaa cggtttaagg attggattgc tattccgcgc
     2581 gataaccttt atcaatcgct gcacacgtcg gtcatcggcg aaggcggaca cgctttcgaa
     2641 gtccagattc gcaccgaaga tatgcaccgg attgccgaag aaggcgtcgc cgcacactgg
     2701 aaatacaaag agaaaaaact cggcaaacac gaagacgacc agaatctgga cgagcttaga
     2761 aaaacggtcg aaaaactgct gctgccgctg gtcgaggaaa cgagcgattt aaacgattca
     2821 caggatttta tcgaatcttt aaagctggat ttgtatccga aagacgttta cgcctttacg
     2881 ccgatgggca aagtcattca gctgccgcgc ggcgcgacgc cgctggattt tgcttacgcg
     2941 attcactcgg aagtcggcga cacgtgtacg ggcgcgaaaa tcggcgggcg catcgtgccg
     3001 ctcaaaaccg agattcaaaa cggcgacgtg gttgagattt taacgagtcc cggctcgcat
     3061 ccgtcgcgcg actggctcaa ttacgtcgcc acttcaaaag cgaaaaaccg cgtccgccac
     3121 tggattgccg aacatcagcg cgccgaatcg atcgaaatcg ggcgcaaact gctttcgacc
     3181 gaagcggaaa aattccgtct gacggcgaaa aaagtgatcg gcgacgagga aaatctgaaa
     3241 cgcatcgcca atgaatacgg cttgggcaga atcgaagatt tgctggcttc catcggttac
     3301 ggcaaaaccc tgccgcgcaa tgtcctcgcc aaatatctgg gcgcggaaaa attcgccgag
     3361 ctcgacccgg agaaaaagaa ggaaacgacg tttcaatcgg gcgtcaaagc cgttaaaaaa
     3421 cttatcggtt ttggcgaaga cgcgattatc gtcaagggcg tcgataatct gcttttaacg
     3481 cgcgctctct gctgcaatcc gcttcgcggc gaagagattg tcggttatat ttcgctcggc
     3541 aaaggcatcg tcgtgcacaa caagcgctgc aaaaacgtgc aggctttgat ggtcaataaa
     3601 gaccgcatcg tcgaggtcga atgggcgaaa atcgacgata agcagattca agccgtgcgc
     3661 ctgctggcga cgaccgaaaa ccgcaccgga atgctcgccg gaatcaccaa cgcgattgcc
     3721 gaaatcaaaa ccggcatccg cgatgccagc gcgcgcgtct cccgtgataa tcaaggctta
     3781 attgaagtga ccattgaagt tttcgacaaa aagcatctcg ataaagtgac cagcgccatc
     3841 aaaaatgttt cgggcgtgat tgacgtcgag cgcgttaatg atttggctta attttataaa
     3901 gtcataagaa acgaaacaaa cgattaaggc tgattatttt ccagaaaaat ccgcctcaac
     3961 cggcgttaat ctactgtttc attctttccg cgcccgcgct gcacactttc cttttcgcct
     4021 ctagtctgtt aaaatcagcc ttttgtttga gtagacgaaa aataaacaga agcaaacagc
     4081 aatgaaagaa attatttcga cggaaaaagc gccgggcgca atcggtcctt attcgcaggc
     4141 ggtcaaaact ggaaatatgg ttttcctttc gggacagatt ccgattgacc cgcaaacggg
     4201 cgaattcgtc gccggcggcg tcgcggaaca aaccgagcag gttttaaaaa atttgagcgc
     4261 ggtgctggaa gcggcgggca cgagcttgaa cgacgtggtg aaaaccaccg tctttttagc
     4321 cgatatgaac gattttacgg cgatgaacga agtttacgcg cgctatttca gcgaaaacaa
     4381 accggcgcgg gcgaccgtcc aggcggcgcg tctgccgcgt gatgcgcgcg tcgagattga
     4441 atgcattgcg attatttaac atttatcatt cgacgtttat cggcgtccga atattggtga
     4501 taaatgttaa atggtaattg ataaatgaaa agccaagcgc gaaagaatca cgcttggcaa
     4561 ccaaaggttc gagttaaatc tcaaactatt ttacacggct ttgacaactt tacccgcttt
     4621 caggcagcga gtgcagactt tttgcctgac cgccgcaccg ctcggcgctt tgacgcgaat
     4681 agattgcagg ttcgggttaa agcgacgacg agttttgttg ttcgcgtgcg aaacgttatt
     4741 tccaaattgc ggaccttttc cgcagacatc acagcgtttt gccatatttt taaagcctcc
     4801 aaaaataaat acgaggtata taagaagagt tttaatataa actcaaaaac aagatttata
     4861 acaaattttt tcaggttaag tcaagtaatg atagatatgg ttaattttga aggagcgaaa
     4921 gccaaagtgc gacaattcag ttttagattt tcagcgattg ctgttttagc catcgccacc
     4981 cttgctttta cggcgtgcgg accgaccacc gggacgggcg gcggacaaac tcccggcggc
     5041 agcgacaccg ccgcgaccgt caacggaaaa gcaatcaaga tggaacaggt cgaaagagtc
     5101 atcaaagccc agacgcaggg acaggacagc cgtctttctc cgcttgagct ggctcaggcg
     5161 cgtttgcagg ttttagacca gctaatccag caggaagtga tgtatcagaa agccgacgcg
     5221 gaaaaaaccg ttccggcgga cgataaagtc accgaagaat tgaacaagct caaacagcag
     5281 agcggaaaat ccgccgaaga gtttgacaag caaatgaagg acgccggcga gagcgaagaa
     5341 tctttgcgcg aatcaatcaa aaagaatctg gcgattcagg cattgaacga aaaagtaacc
     5401 agcaagattg agccgccgaa agaccaggaa atagtagatt ttttcaacgg caacaaggaa
     5461 ggcttcaaga ataagcgcgg cgcgcagctt ggcgtcatcg tcatcgaccc gcgcaaggtc
     5521 gacgccaacg acacgaccgg caatgacatc gaagcgcagc aaaaggcaaa agaaatcggc
     5581 gaccgcttga ttcgcggcgc ggattttgcg accgtcgcgc gcgaagccag cgaagacccg
     5641 cagaccagat tgcagggcgg cgactggcga tatttctcgg aagaagaaat gaaacaggct
     5701 ttcggcggcg gattcgccga gtttgtgatg accaagctgc agaacggtca aatcattccg
     5761 cagacgattc cgtttgaagg caggctgctt atcgtcaagc tccagcgcaa gcaggaacag
     5821 gacgaagacc gcacgctgga gactccgggc gttcgtcagc aaattacgga tttgctgatt
     5881 aattcgcgca aacagcttct gagccaggca tatatggcga tggcgatgga cggcgcgaaa
     5941 gtcgaaaatc tgctggcgaa acaggtcgtc gaaaatccga ataatctgag cggcgcgcgt
     6001 ccggcgtcga cggaaccggc ggcgagtccg acagcggcgg caaacacaaa tatcaacacg
     6061 aacgtcaacg tgaatgccgc gcccgccaaa ccggcaaacg caaacacgaa accggcgacg
     6121 gttccggcgg caaatacaaa cgcgaacgcg agaagatagc ttcgcgccga atctaaaaat
     6181 gacaggtgac agggaaacct gtggcttgtc atttttgttt gataaacgaa tcagaaacgc
     6241 ctgcggcagc gggcgattta attttaaagt ttcgagttat aaatttcggt ttcaccttta
     6301 tgcaagcttg tgactgaaaa ccggaaattt cagattctga ctttcaatcg ctcgcggcgg
     6361 cggggggcgg ttctgtcctg atatgctttt tcgtctttcc gaagtttaca aatcctacag
     6421 cgc
//
LOCUS       DASWNE010000369         4120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_260147, whole genome shotgun sequence.
ACCESSION   DASWNE010000369 DASWNE010000000
VERSION     DASWNE010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260147"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VGB68_07415"
     CDS             <1..315
                     /locus_tag="VGB68_07415"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0049097.1"
                     /translation="RISLNRFVELTSTAAAKIFGLFPKKGTLAVGSDADIVIFDPEKE
                     HTFGVAHEHMNVDYSSYEGWKVKGKVETVLSRGRVVIENGEHKGKAGDGQFLKRGTCV
                     NI"
     gene            506..1024
                     /locus_tag="VGB68_07420"
     CDS             506..1024
                     /locus_tag="VGB68_07420"
                     /inference="COORDINATES: protein motif:HMM:NF013118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049098.1"
                     /translation="MIFKLTERDLKTRFGVFREILYYDGQAETVALVTGDVEAQENVF
                     CRIHSSCISAHVFNSIECQCREEMESSQSLIEQKGRGVIIYLEQEGKGNGHLALMASI
                     PYKKAGLSQSEAYRKAGFQADARSYRAAAEILSDLKVKSVVLLTNNAEKAEDLRKASI
                     IVSGTKQILPGA"
     gene            1124..2047
                     /locus_tag="VGB68_07425"
     CDS             1124..2047
                     /locus_tag="VGB68_07425"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0049099.1"
                     /translation="MDSSPIALASADEQSFDNPAQLSTARQVIEFYEEAGMDYEHWSK
                     NFNMHLGFYRRGLNPFKREKMLEQLNLEISARLQLDAAAAEFLVDMGCGAGAISRTIA
                     RNYPKAIIKGVTLAPSQVETAIKLNVRENLQEQIEIFKGDYASLPFSGGAADGVWAVE
                     SACYAEGAAKENLAREMARVLKTGGRFVVADCFIKQPEKKFNALIEKCYSELCRSWAL
                     PEMPALDYFVEALKTEGFREIVVEDISWRVAPSIAHAPFAVFTFILKKIFAGEPLSRQ
                     SINNLKASLLAPALGLNRSKFSYCLISGKRG"
     gene            2132..3667
                     /locus_tag="VGB68_07430"
     CDS             2132..3667
                     /locus_tag="VGB68_07430"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HEY0049100.1"
                     /translation="MTNIETIQHEDGRVELREAALREIETSPLYNEDLAPVPVEKRHW
                     TTYNYAALWISMAHCIPTYMMASGLISAGMNWWQALLTILLGNTIVLAPILLNSHPGT
                     KYGIPFPVFARAAYGTYGSNLPALMRALVACGWFGIQAWIGGQAIHIFFSSIIPGWNT
                     LLGGAIGGHTPTEWISFLIFWAMNIFIIYRGMNLLRHVENWAAPFVLVMTAILLVWIL
                     YQAGGVGFLLNEPGKFQTAGEFWAIFIPSLTAMIGFWATLSLNMPDFTRFGKSQREQV
                     VGQTVALPTTMVVFAAMGILITSAAVIVFPNAKPDELWDPVKLVGQFSQPLIVAISMF
                     TIVVATLSVNIAANVVSPANDFANAFPRFISFRTGGLITGIVGILMQPWKLLADPSGY
                     IFSWLLGYSGGLGSIAGVLIADYWFVRRKNLNLGDLYRTGGAYRYAGGWNWRAAAATV
                     LGCFFAWIGLIIPSLRILYDYAWFVGFGVSFVVHWALMKAFPPNETAAEVTENTERIR
                     NRE"
     gene            3750..>4120
                     /locus_tag="VGB68_07435"
     CDS             3750..>4120
                     /locus_tag="VGB68_07435"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEY0049101.1"
                     /translation="MSRIIKCGLIQAKNVAPTDAPIAEIKKANIEHQMKMVEEAARRG
                     VQMLCFQEIFTTPYFCAEQQTRWYEAVEKIPDGPTVKMMQDVAREYGMVLIVPIYEEE
                     ITGIYYNSAAVIDADGKYLGK"
BASE COUNT         1029 a          976 c         1122 g          993 t
ORIGIN      
        1 cgaatttcgc tgaatcgctt cgtcgaactg acttcgaccg ccgccgcgaa aatattcggg
       61 cttttcccga aaaaaggaac actcgcggtc ggctcggatg ccgacatcgt gattttcgac
      121 ccggaaaaag aacacacttt cggcgtggcg cacgaacata tgaacgtgga ttattcgtcc
      181 tacgaaggct ggaaagtaaa aggcaaagtc gaaacggttt tatcgcgcgg gcgcgtcgtc
      241 attgaaaacg gcgagcacaa aggaaaggcg ggcgacgggc aatttctaaa acgcggaact
      301 tgtgtaaata tttgaccgcg aggcgattga aagctctcgc aggtcgaacg cttaattaaa
      361 aaccgaatcg agaataatat ctataatcaa tatggacact ttcattctaa atacaaggac
      421 ataacaaatg aagctcttga agcgtatctg gaaagactaa tcttttggac aaagaaaacc
      481 aacgagcttc ttaacctgaa ttgagatgat ttttaaactt acggaaagag acttaaaaac
      541 cagattcgga gtttttcgcg agattcttta ttacgacggg caggcggaaa ccgtcgcgct
      601 ggtgacgggc gatgtggaag cgcaggaaaa cgtcttttgc cggattcatt cgagctgtat
      661 ttccgcacac gtctttaaca gcatcgagtg tcaatgccgc gaggaaatgg aatcatcgca
      721 atctctcatc gagcaaaaag ggcgcggcgt cattatttat ctggaacagg aaggcaaagg
      781 caacgggcat ctggctttga tggcgagtat tccttataaa aaagccgggc tgagccagag
      841 cgaggcttac agaaaagccg gttttcaagc cgatgccaga agctatcgcg cggcggcgga
      901 gattttgtcg gacttaaaag ttaaatcggt cgttctgctg acgaataatg cggaaaaagc
      961 ggaagatttg cgaaaggctt ccattatcgt ctccggcacc aaacagatat tgcccggagc
     1021 gtaattttcg acgaataatc agaaaattac gctcgattat tatcgaacct cgaatttgcc
     1081 cggcgcggcg cgggcgttcc gaaagtataa ataaatatat tatatggaca gcagcccaat
     1141 cgccctggcg agcgccgacg aacagagttt tgataatccc gcgcaattat cgacagcccg
     1201 gcaggttatc gagttttacg aagaagcggg aatggattac gagcattgga gcaaaaactt
     1261 caatatgcat ctgggctttt accgtcgcgg cttaaatcct ttcaagcgtg aaaaaatgct
     1321 cgaacaattg aatctggaaa tctccgcgcg gctgcaactt gacgccgcag cggcggagtt
     1381 tctcgtcgat atgggctgcg gagccggagc gatttccaga acaatcgcca gaaattatcc
     1441 caaagcgatt atcaaaggcg tcacgctggc gccttcacag gtcgaaacgg cgataaaact
     1501 aaacgtcagg gaaaacctgc aagagcaaat cgaaattttc aagggcgatt acgcgtcgct
     1561 gccgttttcg ggcggcgcgg cggacggcgt ctgggcggtt gaaagcgctt gttacgccga
     1621 aggcgcagcc aaagaaaatc tcgcccgcga gatggctcgc gttttgaaaa cgggcggtcg
     1681 gtttgtcgtc gccgattgtt ttatcaaaca gcccgaaaaa aaatttaacg cgctgataga
     1741 aaaatgttat tcggagcttt gccgcagctg ggcattgccg gaaatgcccg cgctcgatta
     1801 ttttgtcgag gctttgaaaa cagaaggctt tcgagagatt gtcgtggaag atatttcgtg
     1861 gcgcgtcgct ccgtctatag ctcacgcgcc gttcgccgtt tttaccttta tcctcaaaaa
     1921 aatcttcgcc ggagagcctt tgagccggca gagcataaac aatctgaaag cttctctgct
     1981 ggctccggcg ctcggtttaa acaggtcgaa gttcagctat tgcttgatta gcggaaagcg
     2041 cggctaaacg gtaattgccg ccgcgacgga aatttaactc gcgcccgatt tccgccgtaa
     2101 acataatacg taagttgcag aaattaaatt tatgacaaac atcgaaacaa tccaacacga
     2161 agacgggcgc gtcgaactgc gcgaggcggc actgcgggaa atcgaaacct ctccgctcta
     2221 caacgaagac ctcgctcccg tgccggttga aaaacgccat tggacgacct acaactacgc
     2281 cgcgctctgg atttcgatgg cgcactgcat cccgacttat atgatggctt ccggtctgat
     2341 ttcggcggga atgaactggt ggcaggctct tctgacgatt cttctgggca acacaattgt
     2401 gctcgcgccg attcttttga attcgcatcc gggcacgaaa tacggcattc cgtttccggt
     2461 ttttgcgcgc gccgcctacg gcacttacgg ctcgaacctg ccggcgctga tgcgggcgct
     2521 ggtcgcctgc ggctggttcg ggattcaagc ctggattggc gggcaggcga ttcacatatt
     2581 tttcagctcg ataattcccg gctggaatac gctgctcggc ggcgcaatcg gcgggcacac
     2641 gccgaccgag tggatttcgt ttctgatatt ctgggcgatg aatattttta ttatttatcg
     2701 cggaatgaat cttttgaggc acgtcgaaaa ttgggcggcg ccgtttgttc tggtgatgac
     2761 cgcgattttg cttgtctgga ttttgtatca agcgggcggc gtcggatttc tgctgaacga
     2821 gccgggaaaa tttcagacgg cgggcgaatt ctgggcgatt tttattccca gcctgaccgc
     2881 gatgatcggt ttctgggcga cgctttcgct caatatgccg gattttacgc gcttcggaaa
     2941 aagccagcgc gagcaggttg tcgggcaaac ggtcgcgctg ccgacgacga tggtcgtttt
     3001 cgccgcgatg ggaattttga taacttcggc ggcggtcatc gtttttccga acgccaaacc
     3061 ggacgagctt tgggacccag taaagctggt cgggcagttt tctcagcctt tgattgtggc
     3121 gatttcgatg tttacgattg tcgtcgcgac gctttcggtc aacattgccg ccaacgtcgt
     3181 ctcgcccgcc aacgatttcg ccaacgcttt tccgcgtttt atttcttttc gcacgggcgg
     3241 cttgataacg ggaatcgtcg gaattttgat gcagccgtgg aaacttttgg ctgacccgag
     3301 cggttatatt ttcagctggc tgctcggata ttcgggcggt ttgggctcga ttgccggagt
     3361 tttgattgcg gattactggt ttgtccggcg aaaaaattta aatttaggcg atttgtatcg
     3421 caccggcggc gcttaccgat acgcgggcgg ctggaactgg cgcgcggcgg cggcgaccgt
     3481 tttgggttgc tttttcgcgt ggattgggct gataattcca agtctcagaa ttttgtatga
     3541 ttacgcctgg tttgtcggtt tcggagtttc attcgtcgtt cactgggctt tgatgaaagc
     3601 ctttccaccg aatgaaacag ccgcagaggt tacagaaaac actgagagaa tccgaaaccg
     3661 cgaatagaca cggataaata tggattttac tcaatatatg tccggcgagc gccgcgcccg
     3721 ctgcggcaat aaaataaagg aatgatttta tgtcaagaat tataaagtgc ggtcttatcc
     3781 aagctaaaaa cgttgcgccg accgatgcgc cgattgcgga aatcaaaaaa gccaacatcg
     3841 agcaccagat gaaaatggtc gaagaagcgg cgcgccgcgg cgtgcagatg ctttgtttcc
     3901 aagagatttt cacgacgcct tatttctgcg ccgaacagca gacgcgctgg tacgaagcgg
     3961 tcgaaaaaat tcctgacgga ccgacggtga aaatgatgca ggacgtcgcc agagaatacg
     4021 ggatggtttt gattgtgccg atttacgagg aagaaatcac gggaatttat tataattccg
     4081 ccgccgtcat cgacgccgac ggcaaatatc tgggcaaata
//
LOCUS       DASWNE010000370         2342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_397441, whole genome shotgun sequence.
ACCESSION   DASWNE010000370 DASWNE010000000
VERSION     DASWNE010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397441"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2342)
                     /locus_tag="VGB68_07440"
     CDS             complement(<1..>2342)
                     /locus_tag="VGB68_07440"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0049102.1"
                     /translation="ICLLAGGANSIYGQGTTGQISGTVTDQNGAVVQGAGVTLSNQET
                     GLSRQATTGGDGIYSFQLLPPGRYKVEVTASGFQKTEAEAVVNITQTTTLDVQLGVTG
                     GTSNVTVEAEAPVLQTETSQNGRVIEGATLRQLPLPTRNFQQLLTLQAGAQSSVSNNT
                     ELGRGDATISVNGQRTTSNSVRINGIDANSIGTNSTPNIAVPATDSLQEFVVQTSLYD
                     ASNGRNSGGNVEAVTRSGTNQFRGNVYYFFRDRNLTANEPFLKGRGLDKPEFSRHQYG
                     MTLGGPVVRDRLFFFGSYQGTREKNGYSLVNSLTSPIVPAGLRDDNRTAAALAATFGV
                     PVANINPVSVAILNARLPSGQFAIPSSGAAGLAANAGVVVPQSGVSRFRENQFNANGD
                     FVVSDKHNISAKFFVADNPTFQANYNFAGLGNGERQLIGFGGDLTIKQKLYSITDNYI
                     FSSNVVNQARFGFNRLRVTSVPEEPFTAAQFGITSSLASLFPGAPTIQVAGLDSQFFF
                     GSAPLADQSSRINGYNFSDVLSVTRGNHRLRFGGEYRFSQVKFYFNAFSRGQIIFAGF
                     NNFLTGTGTSLLGSGVFDRYFKVTDTNFFVQDDWKINNRLTLNIGLRYDLYGLPVENQ
                     GRLVNFLFDQVRVGTTAAPALSPNGFVQAEGGQLAGVPTVEKTLVPVDKNNFAPRIGF
                     AYVLDEKLKMVARGGYGIYYDRISTRFANTQLFNYPYFALGVGLPGILRTLQNPFIPL
                     PTPSQFPTAATIPSPLAPLSPIVGVPIAGVYVDPELQTPY"
BASE COUNT          537 a          633 c          690 g          482 t
ORIGIN      
        1 atagggcgtc tgtaattccg ggtcgacgta gacgcccgca atcggtacgc cgacaatcgg
       61 cgaaagcggc gcgagcggcg aaggaatcgt cgctgcggtc ggaaactgcg aaggcgtcgg
      121 aagcggaatg aacggattct gaagcgtccg caaaattccc ggaagcccaa cgccgagagc
      181 gaaatacggg taattgaaaa gctgcgtgtt ggcaaagcgc gtcgagatgc ggtcgtaata
      241 aatgccgtag ccgccgcgag cgaccatttt taatttttcg tccaaaacgt aggcgaaacc
      301 gatgcgcggc gcgaaattgt ttttatcgac cggaacgagc gttttttcga ccgtcggaac
      361 gccggcaagc tgcccgcctt cggcttgcac gaatccattc ggcgacagag cgggcgcagc
      421 agtcgtgccg acgcgcacct ggtcaaacag gaaattcacg agcctgccct gattttcgac
      481 cggcaaaccg tataagtcgt agcgcaagcc gatatttaac gtcagtcgat tgttgatttt
      541 ccagtcgtcc tgcacaaaaa agttcgtgtc cgtgactttg aaataacggt cgaagacgcc
      601 cgaaccgagc aaggatgttc ccgttccggt cagaaaatta ttaaaaccgg cgaagataat
      661 ctgcccgcgc gaaaaagcgt taaagtaaaa tttcacctgc gagaagcgat attcgccgcc
      721 gaagcgcagg cggtgattgc ctctcgtgac ggaaagcacg tcgctgaaat tgtatccgtt
      781 gatgcgcgaa gactggtctg ccaaaggagc ggaaccgaag aaaaactgcg agtcgagtcc
      841 ggcgacctga atcgtcggcg cgccgggaaa caaggatgcg agcgagctgg taattccaaa
      901 ttgcgctgcg gtaaaaggct cttccggcac gctcgtcacg cgcaggcgat taaagccgaa
      961 acgcgcctgg ttgacgacgt ttgacgaaaa aatatagttg tcggtaatcg aataaagctt
     1021 ttgcttgata gttaaatcgc cgccgaaacc gataagctgg cgctcgccgt tgccgagtcc
     1081 ggcgaaattg taattggctt gaaaagtcgg gttgtcggcg acgaaaaact tggcggaaat
     1141 gttgtgtttg tcgctgacga caaaatcgcc gttggcgttg aattgatttt cacgaaaacg
     1201 cgaaacgccc gattgcggaa cgacgacgcc ggcgtttgcc gccagtcccg cagcgcccga
     1261 cgaaggaatc gcaaattgtc cgctcggcag acgcgcgttc aaaatagcga cggaaaccgg
     1321 attgatattc gccaccggaa cgccgaaagt cgccgccaga gccgccgccg tgcggttgtc
     1381 gtctctgaga cctgccggaa caatcggcga agtcaggctg ttaacgagcg agtacccgtt
     1441 tttttcgcgc gttccctgat acgagccgaa aaagaataag cggtcgcgga cgaccggtcc
     1501 gccaagcgtc ataccgtatt gatgacgcga gaattcgggc ttgtccaagc ctctgccttt
     1561 gagaaacggc tcgtttgccg tcagatttcg gtcgcggaaa aaataataga cgttgccgcg
     1621 aaactggttt gtgcccgaac gcgtcacggc ttcgacgttg ccgccggaat tgcgaccgtt
     1681 cgaagcgtcg tagagcgatg tctgcacgac gaattcctga agactgtcgg ttgccggaac
     1741 ggcgatgttc ggcgtcgagt tcgtgccgat cgagttggcg tcgatgccgt tgatgcggac
     1801 gctgttcgag gtcgtgcgtt gaccgttgac ggaaatcgtc gcgtcgccgc gtccgagctc
     1861 ggtgttattg gaaacggaag actgcgcgcc ggcttgcaaa gtcaggagct gctgaaaatt
     1921 acgcgtcggc agcggcaatt ggcgaagcgt cgcgccttcg atgacgcgcc cgttctgcga
     1981 agtttcagtc tgcaaaaccg gcgcttcggc ttcgaccgtg acgttcgacg ttccgcccgt
     2041 cacgccgagc tgcacgtcca aggtcgtcgt ctgagtaata ttgacgaccg cttcggcttc
     2101 ggttttttga aatccgcttg ccgtcacctc gactttataa cgtcccggcg gcagcagctg
     2161 gaatgaataa atgccgtcgc cgccggtcgt cgcctgacgc gacaaacccg tctcctgatt
     2221 cgaaagggta acgcctgcgc cctgcacgac cgcgccgttc tggtcggtca cagtgccgct
     2281 gatttgtccg gtcgtgccct gtccgtaaat cgaatttgcg ccgcctgcca gcaaacaaat
     2341 aa
//
LOCUS       DASWNE010000371         1988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_436811, whole genome shotgun sequence.
ACCESSION   DASWNE010000371 DASWNE010000000
VERSION     DASWNE010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436811"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VGB68_07445"
     CDS             complement(<1..347)
                     /locus_tag="VGB68_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049103.1"
                     /translation="MIQTASETLQKFQDSVGIKFQLYNSLFLSLPFYGVDKTGILLSL
                     FSSDCEEGFAKDKSPMEIIDNFFENHAAPEKIGLLFRFVQYAERQVVLFDALEDAAFG
                     EINDTQGNGTLQHL"
     gene            408..728
                     /locus_tag="VGB68_07450"
     CDS             408..728
                     /locus_tag="VGB68_07450"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0049104.1"
                     /translation="MKSFYRIERWRQPFAPNPAMLRRILESEGYAVFQWCDQPNATYG
                     LHKHGEDQAHWIVSGKLELTVEAVGTFVLAAGDRDFMPAHTYHTARVISEQAVVYLIG
                     EKLK"
     gene            809..>1988
                     /locus_tag="VGB68_07455"
     CDS             809..>1988
                     /locus_tag="VGB68_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049105.1"
                     /translation="MNKARTLGELKAGGYGARSVKDEMRANLIKKLRAGERLFQGILG
                     YEETVVPQITNAILARHNLILLGLRGQAKSRIIRQLTELLDEEMPIIAGSEINDNPFQ
                     PLSAYGKQQIDVHGDETKIDWVRRENRFVEKLATPDVTIADIIGDIDPIKAAKGGHLL
                     SDELTIHFGLLPRANRGIFAINELPDLAGKIQVGLFNIMQEGDVQIKGYPVRLPLDVM
                     LVFSANPEDYTARGKIITPLKDRIGAEIQTHYPKDIGLGASITKQEAWTKRGDFEGFT
                     VEIPDFIAETIEQIAFEARDDQRIDKRSGVSQRFPITALEAAVSNAERRALITGEAEI
                     VPRISDIYAALPAMTGKMELEYEGEQIGATRLAKDLIKRACGVIFEGFFLGIDFAPTV
                     Q"
BASE COUNT          524 a          460 c          552 g          452 t
ORIGIN      
        1 agatgctgga gcgttccgtt gccctgcgtg tcgtttattt cgccaaacgc ggcatcttcc
       61 agcgcgtcaa ataaaacgac ctgacgctcg gcgtattgga caaagcggaa aagcagaccg
      121 attttttcgg gcgcggcgtg attttcaaaa aaattgtcga taatctccat cgggctttta
      181 tctttggcaa agccttcttc gcaatcgctg ctgaaaagcg aaagcaaaat gcccgtttta
      241 tcgacgccgt aaaagggcag agagagaaac aaactgttgt aaagctgaaa tttgatgccg
      301 accgaatcct gaaatttttg cagtgtttcc gaggctgttt gaatcataaa aaaattgtaa
      361 tatagagtgg aaacgaaaca aacaagacag tcgcggaatt ttaagttatg aagtcatttt
      421 atcgaatcga acgctggcga caacctttcg cgccgaatcc ggcgatgctg cggcggattc
      481 tggaatccga aggctacgcg gtttttcaat ggtgcgacca gccgaatgcg acctacggtt
      541 tgcataaaca cggcgaagac caggcgcatt ggatagtcag cgggaagctg gagctgacgg
      601 tcgaagccgt cggaactttt gttctggcgg cgggcgaccg cgattttatg cccgcgcaca
      661 cgtatcacac ggcgcgcgtt atcagcgagc aagcagttgt ttatttgatt ggcgaaaagc
      721 taaaataagg cagaaggcaa aagtaaaaag gtaaaagttt atctgttttc ctctatgtct
      781 ttatatctct ctgggtaaaa taattgatat gaataaagca cgaacattgg gtgagttaaa
      841 agcaggcggt tacggcgcgc gttcggtcaa agacgaaatg cgggcgaatc tgattaaaaa
      901 acttcgcgcg ggcgaaaggc tttttcaagg cattctgggc tatgaagaaa cggtcgtgcc
      961 gcaaatcacg aacgccattc tggcgcggca caatctcatt ctgctggggc ttcgcgggca
     1021 ggcgaaaagc cgcattatca ggcagttgac cgagctgctg gacgaggaaa tgccgattat
     1081 cgccggttcg gaaatcaacg acaatccgtt tcagcccttg agcgcctacg gaaaacagca
     1141 aatagacgtt cacggcgacg aaacgaaaat cgattgggtg aggcgcgaaa atcgttttgt
     1201 cgaaaaactc gccacgcccg acgtgacgat tgccgacatt atcggcgaca tcgacccgat
     1261 aaaagcggcg aaaggcgggc atctgctctc ggacgagctg acgattcatt tcggcttgct
     1321 gccgcgcgcc aatcgcggga tttttgcgat caacgaactg ccggatttgg cgggaaaaat
     1381 ccaggtcggg cttttcaaca ttatgcagga aggcgacgtg cagataaaag gttatccggt
     1441 gcgcctgcct ttggacgtga tgctggtttt ttcggcaaac ccggaagatt acacggcgcg
     1501 cggaaaaatc atcacgcctt tgaaagaccg cataggcgcg gaaattcaga cgcattatcc
     1561 gaaagacatc ggactcggag cttcgattac gaaacaggaa gcctggacga agcgcggcga
     1621 ttttgaaggt tttacggttg aaattcccga ttttatcgcc gagacgattg agcaaatcgc
     1681 ctttgaagcg cgcgacgacc agcgcattga caagcgttcc ggcgtttcgc agcgttttcc
     1741 gattacggct ttggaagcgg cggtttcaaa tgccgagcgt cgcgctttaa taaccggcga
     1801 agcggaaatc gtgccgcgca tttccgatat ttacgccgcg ctgccggcga tgaccggaaa
     1861 aatggagctt gaatatgaag gcgagcaaat cggcgcgacg cgtctggcga aagatttaat
     1921 caagcgcgcc tgcggcgtga tttttgaagg cttttttctg ggaatcgatt ttgcgccgac
     1981 cgtgcaat
//
LOCUS       DASWNE010000372         6229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_682293, whole genome shotgun sequence.
ACCESSION   DASWNE010000372 DASWNE010000000
VERSION     DASWNE010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6229
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682293"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VGB68_07460"
     CDS             complement(<1..197)
                     /locus_tag="VGB68_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylphosphate carboxylase subunit delta"
                     /protein_id="HEY0049106.1"
                     /translation="MDKSILTADIVARAKKIKLLLMDCDGVLTDGRLYFTESGEAMKV
                     FHVRDGQGLALWHKAGFRSGI"
     gene            complement(287..2539)
                     /locus_tag="VGB68_07465"
     CDS             complement(287..2539)
                     /locus_tag="VGB68_07465"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0049107.1"
                     /translation="MKKLSIIVFTFLLFTFALNVVAQDENVRAAAAWQVKKYDITATL
                     PAAERFLTAKAVLNLQNVGNAAGSRLTLRVSSNAEVSSVAVNNSPAAFTKGEEKLGTS
                     RTLQRIIVTLPSVQPNGTTSVAVDYKLKIDENSGLNTISPVGSQFLPLAFWYPTPNSH
                     YAPRGADFAPFSLTVNAPSGETVVSSGTASANGFEQKLNGQPFFLTGSWDTVEAKGVT
                     VYLPKGAGETEKQRAGELANLAVEAKTFIASLLGGATEVPTRIVAVRRGAGFADAGTI
                     LLDYGALRRQKTDSLTAMTIAEAIAKTWLGNASLVRGEGYGTVREGLSRFIATQFIEK
                     QFGRDVADIERLRQRTAYAAVARRDAPLIQVTPLDDYFYASTANKGAMIWRLVARNLG
                     QTEFFNAIRPTLKEGNLSLASLRAALTTQKESLDYWFDQPTDMNLLVGLPQAVGADIK
                     VALRNLGAIGASVTVIGLTDKGERLTSQVTIPPQSFAEAIFKTSSKIVRAEIDPEKIF
                     PQVDFSDDIAPREFTESDALLVIKRAFDSQKPDVAEKSARTVLNVYPRFDDARIWLAR
                     ALLAQNKTVEAEKEFRAVMEEKLPTGRSLAWANLGLGEIAVKSNQNAQAAKYFEEAIR
                     ADAEYGATLAARAGRSKAASGATIDESVKAFFAQFDKAAVSGTKTNVDALIISGEIPK
                     FAGGVSGAQEWKTTVLQVDKIDANNVLAEVRLNIRLLNREPESGTAVFLLSKAGGSWK
                     LSSVEMFEVR"
     gene            complement(2912..3730)
                     /gene="kdsA"
                     /locus_tag="VGB68_07470"
     CDS             complement(2912..3730)
                     /gene="kdsA"
                     /locus_tag="VGB68_07470"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF003543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEY0049108.1"
                     /translation="MKSFEVKNVTFGDGRLAFILGPCVVESAQHALFMAQEINDICKH
                     AGVDFVYKSSFDKANRSSIESFRGGGIEFGLEVLAQVKEEINVPVITDVHETWQVEKV
                     AEVADIIQIPAFLCRQTDLLIESAKSGKAVNVKKGQFLSPWDAKNIVEKLQNAGGEKI
                     LLTERGASFGYNNLVVDLRSFPIMRSFDVPVIFDVTHSLQLPGGLGKATGGQAEYIEH
                     FARAGVACGVDAVFMEVHDNPAKAPSDGPNQLPLAQLEKLLSKLKAIHELIKDS"
     gene            complement(3748..4035)
                     /locus_tag="VGB68_07475"
     CDS             complement(3748..4035)
                     /locus_tag="VGB68_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049109.1"
                     /translation="MNEFIKNTVSRHSSLKFKANNGEDRVRKSGLKGSLWIKRRYDGF
                     RLQTTSDIATILDGEIERLQGSSAGEQKGYKYWYIDDFHIVEKIIEILARP"
     gene            complement(4050..5768)
                     /locus_tag="VGB68_07480"
     CDS             complement(4050..5768)
                     /locus_tag="VGB68_07480"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEY0049110.1"
                     /translation="MTKYIFVTGGVVSSLGKGLAASSIGCLLESRGARVQMMKLDPYI
                     NVDPGTMSPFQHGECYVTDDGAETDLDLGHYERFTNAKLSQANNWTSGRIYLSVIEKE
                     RRGDYLGKTIQVIPHITDEIKKAVRAVSEKDNPDVLIVEIGGTVGDIESLPFMEAIRQ
                     LGNEEGRNNALYVHVTLVPYIAAAGELKTKPTQHSVRELREIGISADILLCRSEKPLS
                     ADLRRKIALFCNVQETAVIAALDVKTIYEVPLAFHEQGLDDLIVRELNLEEKFPNADL
                     KRWRELVSTIKEPSEGDVKIAIVGKYVELEDSYKSLREALTHAGVANNLRVNVNWIES
                     ESLVDDENYHTRLQDYDAILVPGGFGKRGISGMIRAITYARKSGTPYFGICLGMQTAC
                     IEFARNVCGLRDADSTEFNPETPFPIIFKLRDLVNVEELGGTMRLGAWACQLRENSLV
                     RDIYGGALEIFERHRHRYEFNPEFRQILEQNGFVFSGISPDGKFVEMIELPRETHPFF
                     VGCQFHPEYKSKPVAAHPLFVSFVKAALQNRLSSENLKDDVTSDKQVQREMFESPERV
                     AVMGEE"
     gene            complement(5782..>6229)
                     /locus_tag="VGB68_07485"
     CDS             complement(5782..>6229)
                     /locus_tag="VGB68_07485"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY0049111.1"
                     /translation="RAVAFAIDMLIVVAIISLLIPILNWVLQIPLGYSAVLESSLSLK
                     MDAYTGENFVRLVLIYSLAKLLIVLIYFAGLESSRWQATFGKKIFGFQVGNFEGKRIS
                     TGKAVLRTFGRVLSSQILLIGYLMAFFTKNRQALHDFLAGTFVFKN"
BASE COUNT         1466 a         1677 c         1451 g         1635 t
ORIGIN      
        1 ataattcccg aacgaaaacc cgctttgtgc cacaaggcta agccttgccc gtcgcggacg
       61 tgaaaaactt tcatcgcctc gccgctttcg gtaaaataaa gtcgtccgtc cgtcaaaacg
      121 ccgtcgcaat ccatcaaaag cagcttgatt ttttttgcgc gggcgacgat gtctgcggtc
      181 aaaatagatt tgtccattat ttcaaagcga aaatccatgt gtccttgccg cctgtctgga
      241 ctttccgcct aatttcccaa gatagtttta cttattacta tgtctcttac ctaacctcaa
      301 acatttcgac gctgcttagt ttccagctgc cgccggcttt cgataaaaga aaaaccgccg
      361 tgccgctttc gggttcgcga ttcagcagtc tgatatttaa ccggacttcg gctaagacgt
      421 tattggcgtc gattttatcg acttgcagaa cggttgtttt ccactcctga gcgccgctga
      481 cgccgccggc aaacttcggt atctcgccgg agataatcag cgcgtcaacg tttgttttcg
      541 ttcccgaaac ggcggcttta tcaaattgcg caaaaaatgc ttttacgctt tcgtcgatgg
      601 tcgcgccgct cgcggctttg cttcttccgg cgcgcgccgc caaagtcgcg ccgtattcgg
      661 catcggctct gattgcttct tcaaaatatt ttgccgcctg cgcgttttga ttggatttga
      721 cggcaatctc gccgagtccg agatttgccc aggcgagact tcttccggta ggcagctttt
      781 cctccattac ggcgcgaaat tccttttccg cttcgacggt tttgttttgc gccagaagcg
      841 ctcgcgccag ccagattcgc gcatcgtcaa agcgcgggta aacattcaaa acggttcgcg
      901 cgcttttctc cgctacatca ggcttctgac tgtcaaaggc gcgtttaata actaaaagag
      961 catcgctctc ggtaaattcg cgcggcgcta tgtcgtcgga aaaatcaacc tgcgggaaaa
     1021 tcttttcagg gtcgatttcc gcgcggacta tttttgaaga tgttttaaaa atcgcttccg
     1081 cgaagctttg cggcggaatc gtcacttgcg acgttaatct ctcgcctttg tccgtcaaac
     1141 cgataaccgt cacgcttgcg ccgattgcgc ccagatttct gagcgcgact tttatatccg
     1201 cgccgaccgc ttgcggcaaa ccgaccagca ggttcatatc ggtcggctgg tcgaaccagt
     1261 aatcgagcga ttctttctgc gtcgttaaag ccgcgcgcag gcttgccaaa ctcaaatttc
     1321 cttctttcaa cgtcggtcga atcgcattga aaaattccgt ctgaccgaga ttgcgggcga
     1381 ccagacgcca aatcatagcg cctttgtttg cggtcgaggc gtaaaaataa tcgtcgagcg
     1441 gcgtaacctg tattaaaggc gcgtcgcgcc gggcaaccgc cgcataagcc gtacgctggc
     1501 gaagtctttc gatatcggcg acatccctgc cgaattgttt ttcgataaac tgtgtcgcga
     1561 taaatctcga caagccctcg cgcaccgtgc cgtagccttc gccgcgaact aaagatgcgt
     1621 tgccgagcca cgttttggca attgcttcgg caatcgtcat tgccgtcagc gaatcggttt
     1681 tctgacgccg cagcgcgccg taatcgagca gaatcgttcc cgcgtctgca aagcccgcgc
     1741 cgcgccggac ggcgacgatg cgcgtcggaa cttcggtcgc gccgcctaaa agcgaagcga
     1801 taaaagtttt tgcctcgacg gcgagattag ccagttcgcc ggcgcgttgt ttttcggttt
     1861 cccccgcgcc tttcggcaaa taaaccgtca cgcctttcgc ctcgaccgta tcccagcttc
     1921 ccgtcagaaa aaacggctga ccgtttaatt tctgctcaaa accgttggcg ctcgccgttc
     1981 ccgaagaaac gaccgtttcg ccgctcggcg cattaaccgt caagctaaac ggcgcgaaat
     2041 ccgctccgcg cggcgcgtaa tggctgttcg gcgtcggata ccagaaagcc agcggcaaaa
     2101 attgcgagcc gaccggcgaa atcgtattca agccggaatt ttcgtcaatt tttaatttgt
     2161 aatcgaccgc gacgctcgtc gttccgttcg gctgaaccga cggcagagtg acgatgattc
     2221 tttgcaaagt gcgggaggtg ccgagctttt cttcgccttt cgtaaaagcc gccggcgaat
     2281 tattaaccgc caccgaggaa acttcggcat tcgagctgac acgcaaagtc aggcgcgagc
     2341 cggcggcatt gccgacgttt tgcagattca aaacggcttt cgcggtcaaa aaacgttcag
     2401 ccgccggtaa agtcgcggtg atgtcgtatt ttttgacctg ccacgccgcc gcggcgcgga
     2461 cgttttcatc ctgcgcgaca acgtttaagg caaaagtaaa aagtaaaaaa gtaaaaacta
     2521 ttatcgataa tttcttcatt attaagttcc tgcgtttaaa gcaatctcga atggcttttc
     2581 agaaccgttt tttgtaattt taactttcag ccgggtgcat ttaaatgagc cctcgctcgt
     2641 cccgattttc aaaacgtaat tccctttaaa ataccgctaa tgcaaaattt tctgcgctcg
     2701 tttgttttaa attggctcgc gcgggctgta ccgtgtaaac gcaggtaaag cccgtaaact
     2761 cgttagcgca tttcaaaaaa tcgacgggct ttctaagatg cgtcgataca tccggaattt
     2821 tgagcaaata aattcggaac gaaagttaaa agtaaaaagg caaaagctat tactgaaatt
     2881 ttttcctctt tacgtctttt cataccgcaa tttagctgtc ttttattaat tcgtgaatcg
     2941 ctttcaattt cgaaagcagt ttttccaatt gcgccagcgg caattgattc ggtccgtctg
     3001 acggcgcttt tgccggattg tcgtggactt ccataaaaac cgcatcgacg ccgcaggcga
     3061 caccagcgcg agcaaaatgt tcgatgtatt cggcttgccc gcccgttgcc tttccgagtc
     3121 cgccgggaag ctgcaggctg tgcgtgacgt cgaaaatgac cggaacatcg aacgagcgca
     3181 taatcggaaa gcttcgcaaa tcgaccacca gattattgta accgaagctc gcgccgcgct
     3241 cggtcagaag aattttctcg ccgccggcgt tttgcagctt ctcgacaata tttttcgcgt
     3301 cccagggcga gagaaactgt ccttttttga cgttgacggc tttgccggac tttgccgatt
     3361 cgattaataa atccgtctgg cgacagagaa acgccggaat ctggatgata tcggcgacct
     3421 cggcaacctt ttcgacctgc cacgtttcgt gaacgtccgt gataaccgga acattgattt
     3481 cctctttcac ctgcgcgaga acttccaagc cgaactcgat tccgccgccg cgaaagcttt
     3541 caatcgagct tcgattcgct ttatcgaagg aagatttata gacgaaatcg acgcccgcgt
     3601 gcttgcagat atcgttaatt tcctgcgcca taaaaagcgc gtgctgcgcc gattcgacga
     3661 cgcacggacc taatataaaa gctaaacgcc cgtcgccgaa tgttacattt tttacttcaa
     3721 aagatttcat tacaataatt ttaacggtta aggtcgtgca agaatctcga taattttttc
     3781 aactatatga aaatcatcaa tataccagta tttataacct ttctgctctc ctgcggaact
     3841 gccttgaagc cgttcaattt cgccgtccaa aatagtcgcg atgtcgcttg tagtttgtaa
     3901 acgaaatccg tcgtatcttc ttttaatcca caaactaccc tttaagccgc ttttccttac
     3961 tctgtcttca ccattatttg ctttaaactt tagagaagaa tgacgagaaa cggtattttt
     4021 tataaattcg ttcatatttt ttcattcgat tattcttcgc ccatcaccgc gactctttcc
     4081 ggcgattcga acatttctct ctgcacctgt ttatcgctgg tcacgtcgtc ttttaaattt
     4141 tcgctgctca gacgattttg cagagcggct ttgacgaatg aaacgaaaag cggatgcgcg
     4201 gcgaccggtt tcgatttgta ttcgggatga aactggcagc cgacgaaaaa cgggtgcgtc
     4261 tcgcgcggca gttcaatcat ctcgacaaat tttccgtcgg gcgagatgcc gctgaaaaca
     4321 aagccgttct gttctaaaat ctggcgaaat tccggattga actcgtagcg gtgacgatgg
     4381 cgctcgaaaa tttcaagcgc gccgccgtaa atatcgcgca ccagcgaatt ttcccttaat
     4441 tgacaagccc acgcgcccaa acgcatcgtg ccgccgagct cttcgacatt gaccaaatca
     4501 cgcaatttaa aaattatcgg gaaaggcgtc tcgggattaa attccgtcga gtcggcgtcg
     4561 cgcaagccgc agacgttgcg ggcaaactcg atgcaagccg tctgcattcc cagacaaatg
     4621 ccgaaatacg gcgtgcctga ctttcgggcg tatgtaatgg cgcgaatcat tcccgaaatg
     4681 ccgcgtttgc cgaaaccgcc gggaactaaa atagcgtcgt aatcctgtag ccgcgtgtga
     4741 taattttcgt cgtcgaccag gctttccgat tcaatccagt tgacgttgac gcgcaaatta
     4801 ttcgccacgc ccgcgtgcgt cagcgcctcg cgcagcgatt tatacgaatc ttcgagctcg
     4861 acgtattttc cgacaatggc gattttcacg tcgccttccg aaggctcttt gatggtcgaa
     4921 accagctcgc gccagcgttt caaatcggcg ttcggaaatt tttcttcgag attcagctcc
     4981 ctgacaatca aatcgtccaa gccctgctcg tgaaacgcga gcggaacttc gtaaatcgtt
     5041 ttcacgtcca gcgcggcgat gaccgccgtt tcctgcacgt tacagaacaa agcgattttt
     5101 cgtcttaaat cggcggacag aggcttttcg gaacgacaca aaagaatatc tgcggaaatt
     5161 ccgatttcgc gcaattcgcg gacgctgtgc tgcgtcggct tcgttttcaa ttctccggcg
     5221 gcggcgatat aaggcacgag cgtgacgtgg acgtaaagcg cattgttgcg accttcctcg
     5281 tttccgagct ggcgaatcgc ttccataaac ggcaaagact cgatgtcgcc gaccgttccg
     5341 ccgatttcga caatcaacac gtccggattg tctttttcgc tgacggcgcg caccgctttt
     5401 ttaatctcgt cggtgatgtg cggaatgacc tgaatggttt tgccgagata atcgccgcgc
     5461 cgttcctttt cgataacgga aagataaatg cgcccgctcg tccagttgtt cgcctgcgac
     5521 agcttggcgt tggtaaatct ttcgtaatgc cccagatcga ggtcggtttc cgcgccgtcg
     5581 tcggtcacgt aacattcgcc gtgctggaag ggcgacatcg tgcccgggtc gacgttaata
     5641 tacgggtcga gtttcatcat ctgcacgcgc gcgccgcgcg attccagcag acagccgatt
     5701 gagcttgccg ccaagccttt tcccaagctt gaaactacgc cgccggttac gaatatatac
     5761 tttgtcattt tgttttcccc tttaattttt aaacacgaat gttcccgcca gaaaatcgtg
     5821 caaagcctgt ctgttttttg taaaaaacgc cattaaataa ccgattaata aaatttgact
     5881 ggaaagaact ctgccgaaag ttcttaaaac cgcttttccg gtcgagattc tttttccttc
     5941 aaaatttccg acttgaaatc cgaaaatttt cttgccgaat gtcgcctgcc aacgactgct
     6001 ttccagaccg gcaaaataaa tcaggacgat gagaagcttt gccagcgaat aaatcaaaac
     6061 gagccggacg aaattttctc cggtgtatgc gtccattttc aatgataaac tactttccaa
     6121 aacggctgaa tatccgagcg gaatttgcaa aacccaattc aaaatcggaa tcagcaggct
     6181 gataatcgca acgacaataa gcatatcaat cgcaaaggca accgctctt
//
LOCUS       DASWNE010000373         4976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_542455, whole genome shotgun sequence.
ACCESSION   DASWNE010000373 DASWNE010000000
VERSION     DASWNE010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542455"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /gene="rplT"
                     /locus_tag="VGB68_07490"
     CDS             complement(<1..201)
                     /gene="rplT"
                     /locus_tag="VGB68_07490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEY0049112.1"
                     /translation="MPRAKRGNKRTQKRKKILALAKGFRGTKSKLYRSAKESVERALN
                     FAYTGRKLKKRDFRSLWIVRIGA"
     gene            complement(323..526)
                     /gene="rpmI"
                     /locus_tag="VGB68_07495"
     CDS             complement(323..526)
                     /gene="rpmI"
                     /locus_tag="VGB68_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006163215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEY0049113.1"
                     /translation="MPKLKTHKGAAKRFRSTGSGKFKRGHSHARHILTKKTTKRKRAL
                     DQDVLVSDGDFKRVQTMLPYGRD"
     gene            complement(584..1240)
                     /gene="infC"
                     /locus_tag="VGB68_07500"
     CDS             complement(584..1240)
                     /gene="infC"
                     /locus_tag="VGB68_07500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEY0049114.1"
                     /translation="MIANGFRGRGNRPPFRREPLHRTNERIRVPAVRVIDEEGEALGV
                     MDTRDAIQRARSVGLDLVEIASNAQPPVCRIIDYGKFLYEQKKKQHEAKKKQVTVTVK
                     EIKFRPATDDHDYAYRKERAREWIADGDKVRATIAFRGREMSHRELGSKILAKLRDDL
                     TDIADVEVAPKMEGYQMFAIFIPKKSLKTAGGKSEKKPEKPEKKDIDKEPRSGGDAEK
                     "
     gene            complement(1356..3341)
                     /gene="thrS"
                     /locus_tag="VGB68_07505"
     CDS             complement(1356..3341)
                     /gene="thrS"
                     /locus_tag="VGB68_07505"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEY0049115.1"
                     /translation="MSELNLKFGEQRRQLPAPVSVSDALKAFDRDAAKKALAAKVNGE
                     EVDLNKELAPTDEVLSIEPILAETRDGLEVIRHSTAHLLAASVLDLFPGTKLGVGPAL
                     MDDPRYGFYYDLIAPRQLTEADLPVIEKRMREIAKRNLPYRRENVSRAEIIKLFTERD
                     EPLKCELISEKVEETAEASIYYIDNSPFIDFCLGPHVPHTGKIKAFKLLALSGAYWKG
                     DANNEQMQRIYGTAFATQEELDGWLKQREEAEKRDHRRIGKELDLFSIQDEYGQGFIF
                     WHPKGGAIRTEMENYLREELLKRGYGQVFTPHIAKRQLWQTSGHEENYADSLFEPMGM
                     PEKYGEDTEFRLKPMNCPFHIGIYKAHPRSYRELPLRYAELGTVYRAELSGTLHGLLR
                     VRGFTQDDAHIFCTPAQIEDEIVDCVDFAYDVFKTFGFEKFKVELSVRGGAENKGYLG
                     SDEAWESAESALVNALNRRGIGYERIEGEAAFYGPKIDIKVEDSIGRLWQLSTVQFDF
                     NLPERFQLEFIGDDNQKHRPVMVHRALFGSVERFFGVLIEHFAGAFPFWLAPVQIAVL
                     PITDRINEYAAAAAKELKEAGFRVDLNVKSDKIGAKIREAQMQKVPFMLVLGDKEAED
                     GNVAVRERKAGDLGAMTLEEFKEMARRLKESRALVNV"
     gene            4181..4843
                     /locus_tag="VGB68_07510"
     CDS             4181..4843
                     /locus_tag="VGB68_07510"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0049116.1"
                     /translation="MPKLSEKVIAEKRSVIATAAKELFIKQGFHATSMRDISRRADVS
                     LGNLYNYFETKEAIFESIINGYLTLIDARLKTILEEIDEPLEPTSLRHLGQMAGELVN
                     EHSDFWLLMYIDVLEFRNRHFRKMFDGLADSFRRVFAEKFDSAVQRGDVRKGVDPAVV
                     FTAAYMQFFNYFLVEKLFGGNSHLGLTDEQALNVLTKIFSYGVLSEEKMTQFKKQVIA
                     KV"
BASE COUNT         1236 a         1251 c         1150 g         1339 t
ORIGIN      
        1 cgcgccgatg cggacaatcc aaagcgagcg aaaatcacgt tttttcaatt tgcgtccggt
       61 gtaggcgaaa ttcaacgctc tttccaccga ttccttcgcc gagcgataca gtttcgattt
      121 cgttccgcga aagcccttgg ctaaagctaa tatctttttg cgcttctgtg tgcgcttatt
      181 tccacgtttt gcacgaggca taaatttgtc ctcctaaata aatcctttat aaaaactttt
      241 attaacaatt acgaatttca aattacgaat tacgagtttt aataaaagaa cattctcgta
      301 attcgtaatt gttaattggt aattaatctc ttccgtaggg cagcatcgtc tggacgcgct
      361 tgaaatcgcc gtcggaaacc aaaacgtcct ggtctaaagc gcgtttacgc ttggtcgttt
      421 tcttggtcaa aatatggcgc gcgtgcgaat gtccgcgttt gaatttgccc gaaccggtcg
      481 agcgaaaacg ctttgccgcg cccttgtgtg ttttaagttt cggcataatt actccttcgt
      541 tttaattgcg acgcggagat tgagagattt ggagacgcgg agattatttc tccgcatctc
      601 cgccgctccg tggctctttg tcaatatcct ttttctccgg cttttccggt ttcttttcag
      661 attttccgcc agccgttttt aaacttttct tcggaatgaa aatggcaaac atctggtagc
      721 cttccatttt cggcgcgacc tcgacatcgg cgatgtcggt caaatcgtcg cgcagcttcg
      781 ccaggatttt cgagcccagc tcgcggtgcg acatctcgcg tccgcgaaag gcaatcgtgg
      841 cgcgaacttt atcgccgtcg gcaatccatt cgcgcgcgcg ttccttccgg taagcgtaat
      901 catggtcgtc ggtcgccggt cgaaacttga tttccttaac cgtgacggtg acctgttttt
      961 tcttcgcctc gtgctgtttc tttttctgct cgtaaagaaa ctttccgtaa tcgatgatgc
     1021 ggcataccgg cggctgagcg ttggaagcga tttccaccaa atcaagcccg accgaacgcg
     1081 cccgctgaat cgcgtcgcgc gtgtccatca cgccgagcgc ttcgccttct tcatcaatca
     1141 cgcgcactgc cggaacgcga atccgctcgt tggtgcggtg cagcggctct cttctgaatg
     1201 gcgggcgatt tccgcgccct ctaaaaccgt ttgcgataat ttctctcctt tgttaaaagt
     1261 cggcagagtt ttcagagttg cagatagttg cagagttgtc tgatttgtaa tttaactctg
     1321 caactatctg caactgtccg caacttcctg tcaacttaca catttaccaa agctctcgac
     1381 tctttcagtc gtcgagccat ttccttaaat tcttcgagcg tcatcgcgcc taaatcgccg
     1441 gctttgcgct cgcgcacggc gacattgccg tcttccgctt ccttgtcgcc caaaacgagc
     1501 ataaaaggaa ctttctgcat ctgcgcctcg cgtatcttcg cgccgatttt atcacttttt
     1561 acgtttaaat caacacgaaa accggcttct ttcaattctt tcgccgccgc tgcggcatat
     1621 tcgttgatgc ggtcggtaat cggcagcacc gcgatctgca ccggcgcgag ccagaaggga
     1681 aatgcgcccg caaaatgttc gatcaaaacg ccgaaaaatc tttcgaccga accgaacaaa
     1741 gcgcggtgaa ccatcacggg gcgatgtttc tgattgtcgt cgccgataaa ttcaagctga
     1801 aaacgctcgg gcagattaaa atcgaactgg acggtcgaaa gctgccacag gcgtccgatt
     1861 gaatcctcga ctttaatatc gattttcgga ccgtaaaacg ccgcttcgcc ttcgattctt
     1921 tcataaccga tgccgcgtcg attcagagcg ttgaccagcg cgctctcggc gctttcccac
     1981 gcttcgtcgg aaccgagata acctttgttt tcagcgccgc cgcgaaccga taattcgact
     2041 ttgaatttct cgaaaccgaa agtcttgaaa acgtcgtatg cgaaatccac gcagtctaca
     2101 atttcgtctt caatctgcgc gggcgtacag aaaatatgcg cgtcgtcctg cgtaaatccg
     2161 cgcactctca gaagcccgtg caaagttccc gaaagctcgg cgcgataaac cgtgccgagt
     2221 tcggcgtacc ggagcggaag ctcacgatac gagcgcgggt gcgctttgta tattccgatg
     2281 tgaaacgggc agttcatcgg tttcagacga aattccgtat cttcgccgta tttttccggc
     2341 attcccatcg gctcaaaaag cgaatcggcg taattttctt cgtgaccgga agtttgccaa
     2401 agctggcgct tggcgatgtg cggcgtgaaa acctgaccgt aaccgcgctt taataattct
     2461 tcgcgcaggt aattttccat ctcggtgcga atcgcgccgc ctttcggatg ccagaaaata
     2521 aatccctgac cgtattcgtc ctgaatcgaa aacaaatcca gctcttttcc gatgcgccgg
     2581 tggtcgcgtt tttcggcttc ctcgcgctgt ttgagccagc cgtcgagctc ttcctgcgtc
     2641 gcaaatgcag ttccgtagat tctctgcatc tgctcgttgt tggcgtcgcc tttccaatac
     2701 gcgccggaaa gcgcgagcag tttaaacgcc ttgattttgc ccgtgtgcgg aacgtgcgga
     2761 cccaaacaaa agtcgataaa cggcgaatta tcgatataat aaatactcgc ctcggcggtt
     2821 tcttcgacct tctcgctgat aagctcgcac tttaaaggct cgtcgcgctc ggtgaaaagc
     2881 ttgataatct cggcacggct tacgttttcg cggcgatacg gcagattcct tttggcaatc
     2941 tcacgcatac gcttttcgat aaccggcaaa tccgcttcgg tcaattggcg cggcgcgata
     3001 aggtcatagt aaaaaccgta acgcgggtcg tccatcagcg cgggaccgac gcccagcttc
     3061 gtgcccggaa acaaatccag aaccgacgcc gcaagcaaat gcgcggtcga atgacgaatc
     3121 acctcaagac cgtcgcgcgt ttccgccagg atgggctcga ttgataaaac ttcgtcggtc
     3181 ggcgcgagtt ctttatttaa atcgacctct tcgccgttga ctttcgccgc cagagctttt
     3241 ttcgccgcgt cgcggtcaaa tgcttttaaa gcatcggaaa ccgatacggg cgcgggcaac
     3301 tgccgccgct gttcgccaaa ttttaagttt agttcgctca tatttttgtt aataattaat
     3361 ggataaaaca actctgccga aacttctcgt gcaaagccgg ttaccgaaaa atgaattaat
     3421 tattaacaac taacaattaa ctaataaaga tttgattcga taagagcgac tttgcctcgt
     3481 gcgtctaaag aagaaacgcc attccgacgc gccattaaaa gcgcgtcgta gtagttcgtg
     3541 tgctggcaaa gttcttattc ggcatcttac caaaaaatag aatccggcgg ttttatctac
     3601 tcgaaaataa attttccgcc ctttcaaagt atgtaagagt aacattttgt gaaaatagtt
     3661 gcaagtgtta attaggctgg acaaagtttt aacttgcagt gtatctcgaa actatttcct
     3721 ttcttcgtac tttgtttccg gcaaggaaaa aggaagcgga ttcgctttct cgtcgtgact
     3781 ttgaggcggt tctccgattc ccggctcgaa gcacacccaa acggctcggg cgacgagcgc
     3841 gccaggcatt ataaaaatcc aaatctatag ataaccagaa catttcgccg ccgtgaaaat
     3901 aaaataacat ttaatgttcc tccgtcgcgg cagattaaac tcgccaacaa ttatgccgcg
     3961 ctgctgccgg cgaaaaaaat atagcttcag cttttaaaag agaaaaatcc gaaagaagcc
     4021 gcgaaaatga cggagttatt tattgcttta tagaaataga aaatgaaata gaaatagaaa
     4081 acgaaaagat attttcaaag tttctaatta aaagtttcta attatctatt gacaaacgtc
     4141 ataaattaag ttattaaaaa tacgaacgtc cgttcacttt atgccgaaac tcagtgaaaa
     4201 agtaattgcg gaaaaacgtt ccgtaatcgc cacggcggca aaagagcttt ttatcaaaca
     4261 gggatttcac gccacttcga tgcgtgacat ctcgcggcgc gcggacgtgt ccttgggcaa
     4321 tctttacaat tatttcgaga ctaaagaagc gatttttgaa tcaatcatca acggctatct
     4381 gactcttatc gacgcccggc tgaaaacgat tctggaggag attgacgagc cgctggaacc
     4441 gaccagtctg cggcatctcg gacagatggc gggcgaactg gtcaacgaac attcggattt
     4501 ctggctgctg atgtatatcg acgttctgga attcagaaat cgtcactttc gaaagatgtt
     4561 cgacggttta gccgacagct tccggcgcgt tttcgccgaa aaatttgaca gcgccgtgca
     4621 gcgcggcgac gttcgcaaag gcgtcgaccc ggcggtggtt ttcacggcgg cgtatatgca
     4681 gttttttaat tattttctgg tcgaaaagct tttcggcgga aattctcatt taggcttgac
     4741 ggacgagcag gcgctgaatg ttttgaccaa gattttcagc tacggcgttt tgagcgaaga
     4801 gaaaatgact caatttaaaa agcaggtcat tgccaaggtc tgaaaagcat aggtctacaa
     4861 agttttcatc gaaagatgaa attttaaatg gcgaaaaaca aaaacggacg ttcgttttta
     4921 tttaaaaaga aaaataggag agcgagatgt tgcggaagac aattttcagt attttc
//
LOCUS       DASWNE010000374         2267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_260367, whole genome shotgun sequence.
ACCESSION   DASWNE010000374 DASWNE010000000
VERSION     DASWNE010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260367"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VGB68_07515"
     CDS             <1..181
                     /locus_tag="VGB68_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049117.1"
                     /translation="NVFLARTYLESFEGYSLFVFTIRSMLFVIILVCAIAFAAGVLPA
                     FRASRLNPIEALRYE"
     gene            405..746
                     /locus_tag="VGB68_07520"
     CDS             405..746
                     /locus_tag="VGB68_07520"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HEY0049118.1"
                     /translation="MIVTTGNEVEMGRIVQYLGVVRGIVVRATGIGAGFIGGLKSIGG
                     GNIEEFSRVCEAARMEAFNRMLKHAQEIGADAIIGMRYDATEFTQGSTEVLAYGTAVK
                     LANKERSEVAR"
     gene            complement(935..1948)
                     /locus_tag="VGB68_07525"
     CDS             complement(935..1948)
                     /locus_tag="VGB68_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049119.1"
                     /translation="MTTATTEQVLPEKFSYSSSIAADVWHPKRDARGYEWWYFDALSD
                     DGREAIVVIFLDNFIFSPRYNKLIESRASGIGSGNPQPATGNPPRFPALVFIYYKDGK
                     PLYRAINEFPSEQFFGDEKTPACRLGENEFKFETAPYGSGYLLKIEATLRKNYKLKAR
                     LEWLSIESDFLPDKNIAVEPNSHNWNLVAPRSDVTGKIEVFQSKGKLKDVHHFRGTGY
                     HDHNTDARWLAETVQDWQWGRAHFTDATAVFYRYKEAAAKPTTKLFLVRNGELRASDA
                     DCEERDYARNIFGMRYPKTLSFSTADDVKFSVRQTKLIDASFFYLRFLSETEIKCPDG
                     SRH"
BASE COUNT          551 a          562 c          621 g          533 t
ORIGIN      
        1 gaacgttttc ctcgcgcgga cgtatctcga atcgtttgaa ggctacagct tattcgtctt
       61 tacgattcgc tcgatgctgt ttgtcataat tctggtctgc gcgattgcgt ttgccgccgg
      121 agttctgccc gcatttcgcg cgagccgcct gaatccgatt gaggctttaa gatatgaata
      181 aaaaataagg gagctttaac cgcagattta cacggataaa ggcggattcg aaccggacgg
      241 agccgagcgc ggcggctcga tgccgcggga taaaagaaac gagtcgattc ggattcggct
      301 tattatcctg tatatcgaca ggcgaagcta tttcatccgg ttcgaatccg cgtttcgagc
      361 tgtttgtatc ctgacggcga ataaataaat aaaagggaga aaaaatgata gttacaaccg
      421 gaaacgaggt ggaaatgggc agaatcgtcc aatatctggg cgttgtacgc ggcatcgtcg
      481 tgcgggcgac gggaatcggc gcgggtttta taggcgggct gaaatcaatc ggcggcggca
      541 atatcgaaga attttcgcgc gtctgcgaag cggcgcggat ggaagctttt aaccgaatgc
      601 tgaagcacgc gcaggaaatc ggcgcggacg cgattatcgg aatgcgctac gacgcgaccg
      661 agtttacgca aggctcgacc gaagttctgg cttacggcac ggcggtgaaa ttagcaaata
      721 aggagaggtc ggaagtcgca agatagaaga taaaattaag aagccggaag cggcggctgc
      781 gatggaaaat atttattccc ggctcccggc tctcgcttcc ggcttaaggc agaaacgagc
      841 ctttgccgtt gcgcccgatg cgcatattgg tcagccagtc gagccagcga tatttcaagg
      901 ctttcggcgc gagatgctcg gtgatggcaa aggcttagtg gcgcgagccg tcgggacact
      961 ttatttccgt ctcgctcagg aaacgcaggt aaaaaaaact cgcgtcaatc aatttggttt
     1021 gacggacgga aaacttaacg tcgtcggcgg tcgagaaact cagcgttttc gggtatctca
     1081 ttccgaatat gttgcgcgcg taatctcgtt cttcgcaatc ggcgtcgctc gcccgcagct
     1141 cgccgtttct gacgagaaaa agctttgtcg tcggcttggc ggcggcttcc ttataacgat
     1201 agaaaaccgc cgtcgcatcg gtaaaatgcg cgcgtcccca ttgccaatcc tgcacggttt
     1261 ccgccagcca gcgcgcgtcc gtgttgtggt cgtgataacc ggtgccgcga aaatgatgaa
     1321 cgtctttcag ctttcccttg ctttgaaaaa cctcgatttt tccggtcacg tccgagcgcg
     1381 gcgcgaccag attccagttg tgcgaattcg gctcgaccgc aatgtttttg tccggcagaa
     1441 aatcactctc aatcgaaagc cattcgagcc tcgcttttaa tttatagttt ttgcgcagcg
     1501 tcgcttcgat ttttaaaaga tagcccgaac cgtaaggcgc ggtttcaaat ttaaactcgt
     1561 tttcgccgag ccggcacgcc ggagttttct cgtcgccgaa aaactgttcg gacggaaact
     1621 cgttgatggc gcgataaagc ggcttgccgt ccttgtaata aataaaaacc agcgccggaa
     1681 atcgcggcgg attgccggtt gccggttgcg gattaccgct cccgataccg gatgcccggc
     1741 tctcgattaa cttgttgtat ctgggcgaaa agataaaatt gtcgagaaaa atcacgacaa
     1801 tcgcctcgcg cccgtcgtcg gaaagcgcgt cgaaatacca ccattcatag ccgcgcgcgt
     1861 cgcgcttcgg atgccagaca tccgccgcga tgcttgacga ataggaaaac ttttcaggca
     1921 gaacctgttc tgtcgttgcc gtcgtcatct gatttttgtc cttaacggaa aatttgaaaa
     1981 caaaaaccta aaacgaaagt taaccgcaaa ccggcgcaat gtcaaaattt ttgtgaaccg
     2041 gtacggagga tttttcaccg ccgcgccgga cgcggagaaa gaaaaagact attcgccggg
     2101 gatgaggcgg cgcggcggcg ataagaaaca gattttgaac atcatcggtt tataaaccgc
     2161 gcgaagcctg cttattccgg gcgaatttct ttctctaaat gacagcgtcg gcgcggcgcg
     2221 gcggctcaat ctttcataaa aaacggcaaa cttttgttta tcgagtc
//
LOCUS       DASWNE010000375         4639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_47706, whole genome shotgun sequence.
ACCESSION   DASWNE010000375 DASWNE010000000
VERSION     DASWNE010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47706"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..234
                     /locus_tag="VGB68_07530"
     tRNA            162..234
                     /locus_tag="VGB68_07530"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:196..198,aa:Glu,seq:ttc)
     gene            400..2139
                     /locus_tag="VGB68_07535"
     CDS             400..2139
                     /locus_tag="VGB68_07535"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0049120.1"
                     /translation="MRKVLVTVLFLIFGLVSINAQTTQNADTAMILPFENTSGKPEFN
                     WVGESFAAALSDLMKVPGLNVVSNDERKIIQQRLQVPLTTLPSLATSLKLARESRATL
                     LISGKYNIVAAQGEVAAAVSVTSKIIRVNEGRFLSEELPDGRKITRDINLSDALANLQ
                     TVEGQLAYQILYQRDKALPFSQNQFVESASKIPSRAFEAYIKGLLTSEADFQSRENYF
                     KNAMRIYSEDEKTKGGTYTAVALELGHFYLNQRKFSEAIDYFSRIPAEDSQYPEAAFY
                     TGLIYWQQNNFEQALAVLRPLADDLKLTSVYNTLGAIAVQASLAEKKNKQKGAALLNE
                     GLELLKKSTASAPDDAQSRFNYGFALFLNNNFTEAAQNLRPALAARPTDGEAYFLLAK
                     SLEKLGDATAADFDNQARRFLTNYAKYETEWTRSKTVDVNLRIQQPPRKDFVSVVITK
                     RQSTPVQAPVNETEALLIEARGFYKAGRDDDAMKSLRRVLASEPMSAEAYLLLGNIHL
                     RRGDMEQAVSSLKTALFWDNRLIEAYVALGKIFLEKNDCLQAQNYSRSALELDPNNQD
                     AVALQRQVERCSK"
     gene            3216..4616
                     /locus_tag="VGB68_07540"
     CDS             3216..4616
                     /locus_tag="VGB68_07540"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HEY0049121.1"
                     /translation="MIYDDMTENINESDAFTKHLGMFTHPKGAHLTRRTQQLGDWLQQ
                     RMDVGTWNYSRVLEKSPAPTTGVKNSYGNVTEKINFGSQDYLGLASHPSIRDAAIRAL
                     NEYGPHSASSAILQGNTGISRKLEQSIADLVRMEHVLLFPTGWAAGFGTIVGLVRPND
                     YVVLDQHAHACLMQGARAATDKHTFFRHNDVADCRRKLEKIRAKDTTNAILVVSEGIF
                     SMDSDSPDVLKLQELCHEFNAVLLLDVAHDLGALGPNGTGQPGLQNMLGKIDLVMGAF
                     SKTFSSNGGFLAAHNLSVKQYISIYGGSYTFSNALSPIQTGVVDEAIRISRSEEGDRL
                     RNKLFDNIRRLRAGFSAKGLECLGEPSPIVPVSVGDEKLAKWTSRALEQNGLIANLVE
                     YPAVARGRARFRFQVMASHSTEQIDSAIQLFCQSLEEARSNFAANEAKSETSGSFGVD
                     LLNMIRGEDQALLQAA"
BASE COUNT         1159 a         1206 c         1119 g         1155 t
ORIGIN      
        1 tttcttttgt gaatttgaca gactaaatcg gcatacgtta ttctacgaat tcgcttatcg
       61 gttgatagcc gtgatgtttg agagctttag attttgcttt tcagaaactc gctcgcgttt
      121 ttctcctttt aagagagttt ctcatccgca gtccgctttg tgaccggttc gtctaggggt
      181 ctaggacacc gccctttcac ggcggcaaca cgggttcaaa tcccgtaccg gttactactt
      241 ttttccgata accttttatt taaaatccgc atcaaacctg ttaaaatatt cgcttccaat
      301 acagagcgaa tttttgtttc aggaaatcaa ccgcaaattc gcgctggtca cgtctgaata
      361 atttctgcaa ccaatccgtg tttttgtggt gaaaaaatta tgcgaaaagt tttagtaacg
      421 gttttatttt taatcttcgg tctcgtttca atcaacgccc agacaacgca aaatgccgat
      481 acggcgatga ttctgccctt tgaaaacaca tcgggaaagc ccgagtttaa ctgggtcggc
      541 gaaagcttcg cggcggcgct ttcggatttg atgaaagttc cgggcttgaa tgtcgtctcc
      601 aatgacgagc gcaaaataat ccagcagcgc ctgcaggttc ctttgacgac tttgccgagc
      661 ctggcgacct ctctgaaact ggcgcgcgaa agccgcgcga cgcttctgat ttcgggaaaa
      721 tacaatatcg tcgccgcgca gggcgaagtc gcggcagcgg tcagcgtaac gtcgaaaatc
      781 attcgcgtca acgaaggtcg atttttgagc gaagaattgc cggacgggcg aaaaatcacg
      841 cgcgacatca atctgagcga cgcgctggca aatctgcaaa ccgtcgaggg tcagctcgct
      901 taccagattt tgtatcagcg cgataaggca ctgccgtttt cgcaaaatca gtttgtcgaa
      961 tcggcgagca agattccgtc cagagctttc gaggcttata taaaaggctt gctgacttcc
     1021 gaagcggatt tccaatcgcg cgaaaattat ttcaaaaacg cgatgcggat ttactcggaa
     1081 gacgagaaaa caaagggcgg aacctacacc gccgtcgcgc tcgaactcgg acatttttat
     1141 ttgaaccagc gcaaattcag cgaagcgata gattattttt cgcgcattcc ggctgaagat
     1201 tcgcaatacc cggaagcggc gttttacacg ggcttgattt actggcagca gaacaatttc
     1261 gagcaggcgc tcgccgttct gcgaccgctc gccgacgatt tgaagctgac cagcgtttac
     1321 aatacactcg gcgcaatcgc cgtgcaggct tcgctggcgg aaaagaaaaa taaacaaaag
     1381 ggagccgcgc ttttaaacga gggcttggag cttttgaaaa aatcgaccgc ctccgcgccc
     1441 gacgatgcgc aatctcgctt caactacggt ttcgcgctgt ttttaaacaa taatttcacc
     1501 gaagccgcgc aaaatctgcg tcccgcttta gccgcccgac cgaccgacgg cgaagcttat
     1561 tttctgctgg cgaaatcgct ggaaaaactc ggcgatgcta ccgccgccga ttttgacaat
     1621 caggcgcgtc ggtttttaac caattacgcg aaatacgaaa ccgaatggac gcgctcgaaa
     1681 acggttgacg tcaatctgcg cattcaacag ccgccgcgca aagattttgt cagcgtcgtc
     1741 ataaccaaac gacaaagcac gcccgtgcag gcgccggtca acgagaccga agctttatta
     1801 atcgaagcgc gcgggtttta caaagccggt cgcgacgacg atgcgatgaa atctctgcgg
     1861 cgcgttctgg caagcgagcc gatgagcgcc gaagcttatc ttctgctcgg caatatccat
     1921 ctgcgccgcg gcgatatgga acaggcggtc agcagtttga aaaccgcgct tttctgggac
     1981 aatcgcctga ttgaagccta tgtcgcgctc ggtaagattt ttcttgaaaa gaacgattgc
     2041 ctgcaggcgc aaaactattc gcgctcggcg ctggagctcg acccgaacaa tcaagatgca
     2101 gtcgctttgc agcgtcaggt cgagcgatgt tccaaatagt gtaacttgtc agccggcagc
     2161 cgagagtttt agattgtaaa tttaccggat tccgctccgt aaattaagct tgaaattttg
     2221 tttcccctcg atatttgctg aggtttgtcg cttatcgcac gccgcccgtc acctgccttg
     2281 aaagttgtgg cacggtacgt gcaataaggt aaatcggcaa gcttgatgca gttaatcgag
     2341 accccgccgc caacacttct ccgagcgtgg tttgccactg cgctggacta accttccgag
     2401 cgttccttca gttgtccttc cgattgtgag cctggtcaga aatgaccggg ctctcctgct
     2461 tttaggcata ctttttcaac aacttacagc ttttcgttga tttaccgcaa aatcccttca
     2521 attatttata aaactttcca atttgacctg ttccaaaaat aagaaatccg gcagatgaca
     2581 aaatccggca cttcgcgtgt gacaaatttt gatgtgtcaa tcattttgac gtttcaaatt
     2641 gaattgtatc gttcaatttg aacagatacg ccgcgcgacc agaatatcga cggcgcgaaa
     2701 gacgaaacgc gaaatttgtt tttttattat ttcgcctttc gcctttctta tttcttaatt
     2761 attaaatttt gcggatttcc gatctaacgc cgtttatcgg tttgacaagc tttatcaatc
     2821 ggaatagtct taaccttcag ttgtatttca agcattcttt tcacacggct tttacaataa
     2881 tcctgtaaca atcagaatac ggtagagata tgttaataac tgtctacctg tatccggcgc
     2941 atagagataa tgcgccgggc gtgcgaatat gtgaatcggt attaccttta ccggtaagtt
     3001 cggctgcaat accatccttt cgtaaataag ccgcgcggaa atttatctga gccggagcat
     3061 aattcttcat tatggctctc tagtaattaa ccctgcaaag aatgagcggg taaacgcctg
     3121 tcatttctct ttttcccaaa atatacaaat caaaactccg ggcgtgcgaa tctgcgccgg
     3181 ttaataagca ttaataaaac agaggagcaa ttttaatgat ttacgacgat atgaccgaaa
     3241 atattaacga atccgacgct ttcaccaaac atctcggtat gtttacgcat cctaaaggcg
     3301 cgcatctgac ccggagaacg cagcagctcg gcgactggct tcagcagcgg atggatgtcg
     3361 gcacctggaa ttacagccgg gttctcgaaa aatcgcccgc tccgaccacg ggcgtcaaaa
     3421 attcctacgg caacgtcacc gaaaaaataa atttcggctc gcaggattat ctcgggctcg
     3481 cctcgcatcc gtcgatacgc gacgcggcta tccgcgcctt gaacgaatac ggtccgcaca
     3541 gcgcgtcgtc cgccattttg cagggcaaca cgggcatttc ccgcaagctc gaacaatcca
     3601 tcgccgattt ggtgcggatg gaacacgttc tactctttcc gacaggctgg gcggcgggct
     3661 tcggaacgat tgtcggactg gtcagaccga acgattacgt cgtgctcgac cagcacgcgc
     3721 acgcctgcct gatgcagggc gctcgcgccg ccaccgataa acatactttc tttcgccaca
     3781 acgatgtcgc ggattgccgg cgcaagctcg aaaaaatccg tgcgaaagat acgaccaacg
     3841 ccattctcgt cgtttccgaa ggcattttct cgatggattc cgacagcccc gacgttttaa
     3901 agctgcagga gctttgtcac gaatttaacg ccgttctttt attggacgtg gcgcacgatt
     3961 tgggcgcgct cggaccgaac ggaaccggac agccgggatt gcaaaatatg ctcggaaaaa
     4021 tcgatctcgt gatgggagct ttctccaaaa ctttttccag caacggcggc tttctcgccg
     4081 cgcataatct ttcggtcaag caatacatca gcatttacgg cggctcgtat actttttcca
     4141 atgctctttc gccgattcaa accggcgtcg tcgacgaggc gattcgcatc tcgcgctcgg
     4201 aagaaggcga ccgcctcaga aataaattat tcgacaacat tcgtcgcctg cgagccggtt
     4261 tttccgccaa aggactcgaa tgcctgggcg agccgtcgcc gattgtgccc gtcagcgtcg
     4321 gcgatgaaaa actggcaaaa tggacgagcc gcgcgctcga acaaaacggt ctgattgcca
     4381 atctggtcga atatccggcg gtcgccagag gacgcgcgcg cttccgcttt caggtgatgg
     4441 caagtcattc gaccgagcag attgattcgg cgatacagct cttctgtcaa tcgctcgaag
     4501 aagcgcggtc gaactttgcc gccaacgaag ctaaatcgga aacaagcggc agcttcggcg
     4561 tcgatttgct gaatatgata agaggcgaag accaagccct tttacaggct gcttaatgtc
     4621 acaagccgcc gccgaaaga
//
LOCUS       DASWNE010000376         4516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_260383, whole genome shotgun sequence.
ACCESSION   DASWNE010000376 DASWNE010000000
VERSION     DASWNE010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260383"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VGB68_07545"
     CDS             <1..269
                     /locus_tag="VGB68_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049122.1"
                     /translation="VAVNRRDEARAILREMQTLAAETYVKPYFLGMMHAALGMRDEAF
                     AYFEKSQAEFDPWLLWFATEPMLDSIRDDARYRDLLRRMKLAGD"
     gene            complement(376..999)
                     /locus_tag="VGB68_07550"
     CDS             complement(376..999)
                     /locus_tag="VGB68_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049123.1"
                     /translation="MTTKSTALAIFIALIIILTASAAFAQEAVKPIDAESQTEAPLPD
                     NSMAVRIGIALPKANFFEAGVNSFQIAASIREIIGARFNGTGIEAVALNARLPQAIAA
                     EAGEKSCFYILQTTLLRKRGGGKLNSIMPAAGINQGAPTLLYEAADLASTTKSKDEFT
                     FEYSLISTDDNSVKARNSFKTKAKADGEDVLSPMLENMLKTVLAAAK"
     gene            complement(1199..2194)
                     /locus_tag="VGB68_07555"
     CDS             complement(1199..2194)
                     /locus_tag="VGB68_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049124.1"
                     /translation="MKHAFINKFKALLTIALCLGALWGTAKAQETEPARSSGLTGSAL
                     PVNAQRVLPQSVPAEVNETLEKIVAGANGKIRRGETEVLVWAGAGYKKSSAPQLARQL
                     QSAWKADGWMFEIGGEQDGVTLFSLLRDGAERRALIGFYGATDEAFVLALTELHKAEG
                     NANARSEELPNTETAPQASVEEPSGKASVESNAALRDLVGKWEKKSTMGGRVNANTGV
                     YLGSSGTYESYEFSADGGVAYSSLISVQQGGCNLSAFSQSRGAAGVSGSKMTINLGAG
                     TIDRKDTCNASGDYKKATRATNYTYEWTIDKDEYGTVQLCLTESNGNKYYYRKVK"
     gene            complement(2227..2718)
                     /locus_tag="VGB68_07560"
     CDS             complement(2227..2718)
                     /locus_tag="VGB68_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049125.1"
                     /translation="MKFIPTFFLAMFLLIATGETFGQKMPSELIGEWHNGSVSMLQEK
                     NTATGQTTPSNGSTFTYKFFADGRFEFVGYMKSTMYGCTTDLFNDKRGKVEIDGDRIT
                     FVPAKNYWKNTYSCAPAGNKEKNHTLEKETYTWRTKEDEYGKTLVCLANAKGESCYRR
                     EEK"
     gene            complement(2853..3896)
                     /locus_tag="VGB68_07565"
     CDS             complement(2853..3896)
                     /locus_tag="VGB68_07565"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY0049126.1"
                     /translation="MNTQNNAAALAPEMPAEAVLGQIIMGAFASQAVYVAARLGIADL
                     LKDNPRPVAELAAATGTDADALYRVLRALASAGVFAETAPRIFALTPFAEPLLSDRPG
                     SMREMAIWMGEEAHWRVYGEMLYSVQTGKPAFERVHGQEAFPYLFETNRALGDIFNRA
                     MTSFSSSVAPAIAQAYDTSEAKTLVDIAGGHGLLLSAFLKANPDLKGVLFDVPTVIED
                     AGALLESEGVAGRVETVTGDFFESVPAGGDVYMMKHIIHDWDDARALAILKNIHSAMN
                     ENGKVLIVEMVVKEGNEPDFSKIMDLEMLVTPGGVERTAEEYRRLLAQAGFRLTRIIP
                     TASPYSIVEAVKA"
     gene            complement(3915..4307)
                     /locus_tag="VGB68_07570"
     CDS             complement(3915..4307)
                     /locus_tag="VGB68_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049127.1"
                     /translation="MSIIRKIACYSLLILSILSAVPSLATTEDRVTIDRDIVFRRGAI
                     SASVRGRINRGTTHLYRVRARAGQEMAIVLRTGEQTSATVFAPTEGIVEGADGVRQTL
                     VELPETGEYLIQIGTDAAANYTLEVTIN"
BASE COUNT         1004 a         1220 c         1162 g         1130 t
ORIGIN      
        1 atgtcgccgt caatcgccgc gacgaagcgc gcgcgatttt gcgtgagatg cagacgctcg
       61 ccgccgaaac ctatgtcaag ccgtattttc tgggaatgat gcacgccgcg ctcgggatgc
      121 gcgacgaggc tttcgcttat tttgaaaaat cgcaagccga attcgacccg tggctgctct
      181 ggtttgcgac cgaaccgatg ctcgattcca tccgcgacga tgcgcgctac cgcgatttat
      241 tgcggagaat gaagctcgcc ggagattgaa aaaccgacaa aataaaaaaa gataaaataa
      301 aagaagacga gaaattttcg ttctcgcctt ctcccgcctt ccgagcgttt tccggtcgtc
      361 gtttgaaact tttttttact ttgccgccgc cagaaccgtc ttcaacatat tttccagcat
      421 cggcgaaaga acgtcttcgc cgtcggcttt cgccttagtt ttaaaagaat ttctggcttt
      481 aacgctattg tcgtcggtgg aaatcaggct gtattcgaaa gtgaattcgt ctttcgattt
      541 cgttgtcgaa gctaaatccg ccgcttcata aagcaacgtc ggcgcgccct ggtttatgcc
      601 tgccgccggc attatcgaat taagctttcc gccgccgcgc tttctcaaaa gggtggtttg
      661 caaaatataa aaacaacttt tttctccggc ttccgcggcg atcgcctgcg gcaggcgagc
      721 gtttagcgcg actgcctcga tgcctgttcc gttaaagcga gcgccgatta tttcccgtat
      781 gctcgccgct atctgaaaac tattcacgcc cgcctcgaaa aaattcgctt tcggcagagc
      841 aataccgatt ctgacagcca tcgagttgtc gggcagcggc gcttcggttt gagattcggc
      901 atcaatcggc ttaacagcct cctgtgcgaa tgccgcgctc gcggtcaaaa ttattatcag
      961 agcgataaag attgcaagtg ccgtcgattt ggtggtcatg atgattttcg caaaaaaaat
     1021 tgagcgagcc ggcggtttat tcgccgctct ttattgttaa ggcgtaaagc gccgccgaaa
     1081 agtatccgcc gggcgagaaa attttcagga atttaataac aaacaataaa aaaagaggcg
     1141 gcttgggcga gccgccgcgg gcgcggctct tttcgattca cgactatttt ttgaaacgtt
     1201 atttcacttt acgataataa tatttgttgc cgttcgattc ggtcaggcaa agctgtacgg
     1261 tgccgtattc gtctttgtcg attgtccatt cgtaagtgta attcgtcgcc ctggtcgctt
     1321 ttttataatc gccgctcgcg ttgcaggtgt ctttccggtc aatcgtgccc gcgccgagat
     1381 taattgtcat tttcgagccg gagacgccag ccgcgcccct gctctgggag aaagccgaca
     1441 gattgcagcc gccctgctgg acggaaatca gcgacgaata agcgacgccg ccgtcggcgg
     1501 aaaattcata gctttcgtag gttccgctgc tgcccagata gacgcccgta ttggcgttga
     1561 ctctgccgcc catcgtagat tttttctccc atttgccgac caaatcgcga agcgcggcgt
     1621 tggattcaac ggacgccttt cctgaaggtt cttcgacgct cgcttgcggc gcggtttcgg
     1681 tgttcggcaa ttcttcgctg cgggcatttg cgttgccttc agccttgtgc agctccgtca
     1741 gagccagaac aaaagcttcg tccgtcgcgc cgtaaaagcc gataagcgcg cggcgctcgg
     1801 ctccgtccct gagcaggctg aacagcgtca cgccgtcctg ctcgccgccg atttcaaaca
     1861 tccagccgtc agctttccag gcgctttgca gctgtcgggc aagctgcggc gcgctcgatt
     1921 ttttgtagcc cgcgccagcc cagaccagaa cttccgtctc gccgcggcga attttcccgt
     1981 ttgcgccggc gacgattttt tcgagcgttt cgttgacttc cgccggaacg ctctgcggca
     2041 aaactctctg cgcgtttacg ggaagcgccg agccggtcaa gcctgagctt cgcgccggtt
     2101 cggtttcctg cgctttcgcc gttccccaga gcgcgcccag acaaagcgcg attgtcagca
     2161 gtgctttgaa tttatttata aaagcgtgtt tcataattct cctgatttat tatttaatct
     2221 gcctttttat ttttcctcgc ggcgatagca gctttcgcct ttcgcgtttg ccaggcaaac
     2281 cagagttttg ccgtattcgt cttctttcgt gcgccacgtg taggtttctt tttccagagt
     2341 gtggtttttt tccttgttgc cggcgggcgc gcagctataa gtgtttttcc agtagttttt
     2401 cgccggaacg aaggtgatcc ggtcgccgtc gatttcgact ttgccgcgct tgtcgttaaa
     2461 taaatcggtc gtacagccgt acatcgtcga tttcatatag ccgacgaatt caaaacgccc
     2521 gtcggcgaaa aatttataag taaaagtcga gccgttggac ggcgtggttt gtccggtcgc
     2581 ggtgtttttt tcctgaagca tcgaaacgct gccgttgtgc cattcgccga ttaattcgga
     2641 cggcattttt tgtccgaaag tctcacccgt ggcgattaat aaaaacatcg ctaagaaaaa
     2701 agtcggtata aatttcataa tttcctccgt gaaaaataaa tctcaaatac taaggcgaaa
     2761 caaaagcgaa aaagggttca ccgtaaaagc aaaaatgcgt cgatttctct tgaaatcaac
     2821 gcagatggat aatccggaac cggttgaaac gactaagctt tgacggcttc gacgatgctg
     2881 tagggcgaag cggtcggaat gatgcgcgtc aggcgaaatc cggcttgcgc cagcagccgt
     2941 cgatattctt cagccgtgcg ctcgacgccg ccgggcgtca ccagcatttc caaatccata
     3001 atcttgctga aatccggctc gttgccttct ttgacgacca tctcgacgat taaaactttg
     3061 ccgttttcgt tcatcgccga atgaatgttt ttcaggatgg cgagcgcgcg cgcgtcgtcc
     3121 caatcgtgga tgatatgttt catcatataa acatcgccgc ccgccggaac gctttcgaaa
     3181 aaatcgcccg tcacggtctc gacccttccg gcgacgcctt cgctttcgag cagcgcgcca
     3241 gcgtcttcga tgaccgtcgg cacgtcgaac aaaacgcctt ttaaatcggg attcgctttc
     3301 aaaaacgccg aaagcagaag cccgtgaccg cccgcgatat cgaccagcgt tttcgcttcc
     3361 gacgtatcat aagcctgcgc gatagccgga gcgacgctcg acgaaaagct cgtcatcgcg
     3421 cggttgaaaa tatcgcccag cgcgcggttc gtttcaaata aatacggaaa agcctcctgt
     3481 ccgtgaacgc gttcaaaagc gggtttgccc gtttggacgc tgtagagcat ctcgccgtaa
     3541 acgcgccagt gcgcttcttc gcccatccag attgccatct cgcgcatact gcccggtcgg
     3601 tcgctcaaaa gcggctcggc aaacggcgtc agcgcgaaga ttcgcggcgc ggtctcggca
     3661 aaaacgccgg cgctcgccag cgcgcgcaaa acgcggtaaa gcgcatcggc gtcggtgccg
     3721 gtcgcggcgg ctaattccgc gaccgggcgc ggattgtctt tgagcaaatc ggcgatgccg
     3781 agcctggcgg cgacatagac ggcttgcgac gcaaaagcgc ccatgatgat ttgccccaaa
     3841 acggcttccg caggcatttc cggcgcgagc gcggcggcat tattttgtgt attcataatt
     3901 gcaaattctc cttcttaatt aatcgttact tcaagcgtgt agttagcggc ggcatcggtg
     3961 ccgatttgaa tcaggtattc gcccgtttcc ggcagctcga ccagcgtctg acgcacgccg
     4021 tcagcgcctt cgacgatgcc ttcggtcggc gcgaaaacgg tggcggaagt ctgctcgccg
     4081 gttctcagga caattgccat ttcctgaccg gcgcgggcgc gaacgcgata taaatgcgtc
     4141 gtgccgcgat tgattctgcc tcgcacggaa gcggaaatcg cgccgcgccg gaaaacgatg
     4201 tcgcggtcaa tcgtcacgcg gtcttccgtc gtcgccaaac tcggcacggc ggaaaggatg
     4261 gataaaatca ataatgaata acaggcgatt tttcggataa tagacataat tgctttctca
     4321 cttatcggat ataaaatata aaagattgaa gaaaagtctt ctctcgtaat agcggaaagc
     4381 gcgggcgaaa agtatcaaaa agaataaaaa agacttgaaa aagacggaag cgcgggcaca
     4441 aaacccgtcc ttatatattt ttttacgttg aatctttttc tttaaagaaa gtataatttt
     4501 cgacgaatgc ggaaaa
//
LOCUS       DASWNE010000377         5065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_647695, whole genome shotgun sequence.
ACCESSION   DASWNE010000377 DASWNE010000000
VERSION     DASWNE010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647695"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3056
                     /gene="metH"
                     /locus_tag="VGB68_07575"
     CDS             <1..3056
                     /gene="metH"
                     /locus_tag="VGB68_07575"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEY0049128.1"
                     /translation="EFFETNRVRLPVIAQVTIETFGTMLNGTEISAALTALEPFPIDV
                     VGMNCGTGPKQMTESIKYLCENAPMPVSVLPNAGLPEVKDGAMHYDETPESFTAQVLH
                     FARDFGANIVGGCCGTTPEHLKLVVEQVERLSPKQRGASLVPSASSIFIQQPYTQDNS
                     FLIIGERVNASGSKKMRDLLEKEDWDGLVSLAKEQEREGAHVLDVNVDFVGRDGVADM
                     HELVSRLVTNVKIPLMLDSTEWQKMEAGLQHAGGKCLLNSTNYEDGEERFLKVFELAK
                     EYGAGVVVGTIDEEGMARTAEGKLKIARRAYRQAVEFGIEAHDIFFDPLALPISTGIE
                     EDRRNAAETIESIRQIHNEMPEANILLGVSNVSFGLNPAARVVLNSNFLHECVEAGMN
                     AAIVNASKILPLNRFSEHEVEVALELIYDKRKFDGDICTYDPLGEFTTMFQGKTAKSM
                     KVDVSNLSTEEKLKHHIIDGEKIGLEDNLKKALENYPALDIVNDILLDGMKTVGDLFG
                     SGQMQLPFVLQSAEVMKTAVKFLEPFMDKVEGESNKGVMVLATVKGDVHDIGKNLVDI
                     ILTNNGYKVVNLGIKQPIEEILKAFEETGCDAIGMSGLLVKSTLIMRDNLEIMNERGI
                     NVPVVLGGAALNRRYVDQDLVPLYNGKLFYARDAFDGLHAMDALTSRESKVESEESKA
                     RVAAAGNGSETAATAGGLSADSIQTVEDAEDLVGEDAKLGKVAARVRARISGDTTHTK
                     KSDVSADVEIPAAPFYGSRVVEDIDLEEVFAFINETALFKGQWQYKQGRKTKEEYEKI
                     LFETVYPKFAAVKEKSKLENLLTPKVVYGYFPCQSEGNDLIIYDRDEKTERLRFTFPR
                     QPENQRGGRNLSLADYFASKDSGKTDVVAFDLVTMGRRASEYAAELFKADSYTDYLLF
                     HGLSVESAEALAEMWHQRIRRELGIAGKDAPTLVKLFHQGYQGSRYSFGYPACPRLED
                     QTKIFELLQPERIGVELSEEFQLEPEQSTSAIIVHHPEAKYFNIE"
     gene            3059..3367
                     /locus_tag="VGB68_07580"
     CDS             3059..3367
                     /locus_tag="VGB68_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049129.1"
                     /translation="MDIHSKSTYLSEFKPKNLSKHHSNHEWIFAQYEEPPQQREDGKW
                     YRAFLFRNKDRTVFGKKEFWESTSVDFRQMATRVITDKEFRDGLISDDEDLPKIWKRH
                     "
     gene            3556..4275
                     /locus_tag="VGB68_07585"
     CDS             3556..4275
                     /locus_tag="VGB68_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049130.1"
                     /translation="MAINASLLTRQSDRRLFSIAAIGFPLLVLIGYFKSYYFSAFFDV
                     RPLANALVHAHGIVMTIWVLYFVAQVALIRSKNVKLHMSMGMAGIALAAMVVIIGLAT
                     AYDAQLVRRASPPGTDPHEFFVLPVGDMLLFVVYFAGAIYYRKRPVEHKSLMLMTAIN
                     FAPAALFRIPIVPAEYTLPFVFGAAASIAIVCLAWFKVKHGKFNLIFAAATLLFIIAL
                     PFRLALAETAIWHSFTNWLAS"
     gene            complement(4369..>5065)
                     /locus_tag="VGB68_07590"
     CDS             complement(4369..>5065)
                     /locus_tag="VGB68_07590"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0049131.1"
                     /translation="KFIPWIVEQYENGAEVVSLCVGAFLLAKTGLLTGRKCSTHWRAA
                     DEFRKMFPDVHLVTDKIITEEQGIYTSGGATSYWNLLLYLVEKYTSRETAILTSKVFA
                     IDIERHSQSQFIMFKGQSDHTDENVKKAQKFIENNFQERITLDDLSAKFALSRRSLER
                     RFKKATSNTVSEYIQRVKIEAAKKSFETSRKNITEVMFDVGYSDTKAFRGIFKKITGM
                     TPFDYRNKYNKEV"
BASE COUNT         1250 a         1236 c         1346 g         1233 t
ORIGIN      
        1 ttgaattttt cgagacaaat cgcgtgcggc ttccggtcat cgcgcaggtc accatcgaaa
       61 ccttcggcac gatgctgaac ggcacggaaa tttccgccgc tttgacggcg cttgagcctt
      121 tccctattga cgtcgtcgga atgaactgcg gaaccggtcc gaaacagatg accgaaagca
      181 tcaaatacct gtgcgaaaac gcgccgatgc ccgtttcggt tttgcctaac gccggtctgc
      241 cggaagtcaa agacggcgcg atgcattacg acgaaacgcc ggaaagcttt accgcgcagg
      301 ttctgcattt cgccagagat ttcggcgcaa acatcgtcgg cggctgctgc ggaacgacgc
      361 ccgaacattt gaaactggtc gtcgagcagg tcgaaaggct ttcgccgaaa cagcgcggcg
      421 cgagtttagt tccgtcggct tcgtccattt ttatccagca gccgtacacg caggacaatt
      481 ctttcctgat tatcggcgag cgcgtcaacg cttccggctc gaaaaaaatg cgtgatttgc
      541 tcgaaaagga agactgggac gggcttgtct cgctggcgaa agagcaggag cgcgaaggcg
      601 cgcacgttct ggacgtcaac gtcgatttcg tcgggcgcga cggcgtcgcc gatatgcacg
      661 aactggtttc gcgactggtc acaaacgtta aaattccttt gatgctcgat tcgaccgagt
      721 ggcagaaaat ggaagccggg cttcagcacg cgggcggaaa atgtcttttg aattcaacga
      781 attacgagga cggcgaagaa cgctttctga aagttttcga attggcgaaa gaatacggcg
      841 cgggcgtcgt cgtcggcacg attgacgaag aaggaatggc gcgcacggcg gaaggaaaac
      901 tgaaaatcgc gcgccgcgct taccggcaag ccgtcgaatt cggcatcgaa gcgcacgata
      961 ttttcttcga cccgctcgct ttgccgattt cgaccggcat cgaagaagac cgccgcaacg
     1021 ccgccgaaac catcgaatca atccggcaga ttcacaatga gatgccggaa gcgaatattc
     1081 ttttgggcgt ttccaacgtc tctttcggat tgaatccggc ggcgcgcgtc gttctaaact
     1141 caaactttct gcacgaatgc gtcgaagccg gaatgaacgc ggcaatcgtc aacgcctcga
     1201 aaattctgcc gctgaatcgc ttcagcgagc acgaggtcga ggtcgcgctc gaacttatct
     1261 acgacaagcg aaaatttgac ggcgacatct gcacatacga cccgctcggc gaatttacga
     1321 cgatgtttca gggcaaaacg gcgaagtcga tgaaggtcga cgtttccaat ttatcgaccg
     1381 aggaaaagct caaacatcac atcatcgacg gcgaaaaaat cggcttggaa gacaatctga
     1441 aaaaagctct ggaaaattat ccggcgctcg atatcgtcaa cgatattctg ctcgacggaa
     1501 tgaagacggt cggcgatttg ttcggcagcg ggcagatgca gcttcccttt gttttgcagt
     1561 cggcggaagt gatgaaaacg gcggtcaaat ttctcgaacc gtttatggac aaggtcgagg
     1621 gcgagtcgaa caaaggcgtg atggttctgg cgaccgtcaa aggcgacgtc cacgacatcg
     1681 gcaaaaatct ggtcgatatt attttgacca acaacggcta caaggtcgtc aatctcggca
     1741 tcaaacagcc gattgaagag attttaaaag ctttcgaaga aaccggctgc gatgccatcg
     1801 ggatgagcgg cttgctcgta aaatcgacct tgattatgcg cgacaatctc gaaattatga
     1861 acgagcgcgg cattaatgtt ccggtcgtcc tcggcggcgc ggctctgaat cgccgctacg
     1921 tcgaccagga tttggttccg ctctacaacg gcaaattatt ttacgcgcgc gacgcttttg
     1981 acggtctgca cgcgatggac gcgctgacga gccgggagtc gaaagtggag agcgaagaat
     2041 cgaaagcgag agtcgcggcg gctggaaacg ggtcggaaac ggcggcgacg gcgggcggct
     2101 tatctgccga ctcgattcaa acggtcgaag acgcggaaga tttagtcggc gaagacgcaa
     2161 agctcggaaa agtcgcggcg cgcgtcagag cgagaatttc cggcgacacg acgcacacga
     2221 aaaaatccga cgtttcagcg gatgtcgaaa ttccggctgc gcctttttac ggctcgcgtg
     2281 tggtcgaaga tatagatttg gaagaagtct tcgctttcat caatgagacc gcgctcttta
     2341 aaggtcagtg gcaatacaaa cagggacgca aaacgaaaga agaatacgaa aaaattttgt
     2401 ttgagacggt ttatccgaaa ttcgccgccg tcaaggaaaa atcgaagctc gaaaatctgc
     2461 tcacgccgaa agtcgtctac ggctattttc cctgccaatc cgaaggcaac gatttaatca
     2521 tctacgacag ggacgaaaaa accgagcgat tgcgcttcac cttcccgcgc cagcccgaaa
     2581 accagcgcgg cgggcgcaat ctctcgctgg ctgattattt cgcgtcgaaa gattcgggca
     2641 aaacggacgt cgtcgccttc gatttggtga cgatgggaag acgcgccagc gaatacgccg
     2701 ccgagctttt caaagccgac agctacacgg attatctttt gtttcacggt ctttcggtcg
     2761 aatcggcgga agcgctcgcc gaaatgtggc accaacggat tcgccgggag ctcggaatcg
     2821 ccggaaaaga cgcgcccaca ctcgtaaagc tttttcatca gggctatcag ggctcgcgct
     2881 acagtttcgg ctatccggcg tgcccgcgtt tagaagacca gacgaaaatt ttcgaactgc
     2941 tccagcccga aagaatcggc gtcgaactct cggaagaatt ccagctcgaa ccggaacaat
     3001 cgacttcggc gataatcgtg catcacccgg aggcgaaata ttttaatatc gagtaaaaat
     3061 ggatatacat tcgaaaagca catatttatc cgagttcaag cctaaaaact tgtcaaaaca
     3121 tcactcgaat cacgagtgga tttttgctca atatgaggaa ccgccgcaac aaagagaaga
     3181 tggaaaatgg tatagagctt ttttgtttcg caataaagac agaacggttt ttggtaagaa
     3241 agagttttgg gaatcgactt cagtagattt tcgtcaaatg gctactcgcg tcataaccga
     3301 taaagagttt cgtgacggtt tgatttcaga cgacgaagat ttacctaaga tttggaaaag
     3361 acattaattt tactaaccat ctattgcaga aaaacctgac tttgccgagc cgcaaagtcg
     3421 ggtttttcat tgtaatattt ttaattgcgg caaatttctc cgctgtttgg tcgagagctt
     3481 tataaattct caaaaagaaa atcggtgata cttttcgccg cgattttacg cgtttttaag
     3541 tcggaggttg attttatggc aatcaatgca agcttattaa cgcgacagtc agaccgccgt
     3601 ttattttcaa tagcggcaat cgggtttccg cttctggttt taatcggata tttcaaatct
     3661 tattatttca gcgcattttt tgacgtaaga ccgttagcca acgcgctggt tcacgcgcac
     3721 ggcattgtga tgacgatttg ggttttatat ttcgtcgcgc aggtcgcgct gattcgctcg
     3781 aaaaatgtaa agctgcatat gtcgatggga atggctggca tcgcgctggc ggcgatggtc
     3841 gtcattatcg ggctggcgac ggcgtatgac gcgcagctcg tccggcgcgc ctcgccgccg
     3901 ggaaccgacc cgcacgagtt tttcgttctg cccgtaggcg atatgctttt gttcgtcgta
     3961 tattttgcgg gcgcgattta ttaccgcaaa cgtccggtcg aacataaatc gctgatgctg
     4021 atgacggcga ttaatttcgc gcccgccgca ttattcagaa ttccgattgt gccggcggaa
     4081 tacacgctgc cgtttgtatt cggagccgcc gcctcgattg cgattgtctg tttggcgtgg
     4141 tttaaggtga aacacggcaa attcaacctt attttcgccg ccgcaacctt gctgtttatc
     4201 atcgccttac cctttagact cgctttggct gaaacggcaa tctggcacag ttttacaaat
     4261 tggctggctt catagataaa aaacgcggaa cgatgaacgc cgcagcgatg aattcgatac
     4321 gcagaaactg caattcatcg ttccgcgttc atcattcatc attccatttt agacttcctt
     4381 gttgtattta ttccggtaat cgaacggcgt cattccggtg atttttttga aaattccgcg
     4441 aaaagccttg gtgtccgaat agccgacgtc gaacattact tcggtgatgt ttttgcggct
     4501 ggtttcgaag ctttttttgg cggcttcgat tttgacgcgc tggatgtatt cgctgacggt
     4561 gttgctggtg gcttttttga agcgacgctc caggctgcgg cggctgagag cgaatttcgc
     4621 ggataaatcg tcaagcgtga tgcgctcctg aaaattattc tcgataaatt tttgcgcttt
     4681 tttgacgttt tcgtcggtgt ggtcgctctg acctttgaac attataaatt gcgattggct
     4741 gtggcgctcg atgtctatgg caaagacttt cgaggtgaga atcgccgttt cgcgactcgt
     4801 gtatttttcg accagatata aaagcaggtt ccaataagaa gtcgcgccgc cgctggtgta
     4861 gatgccttgt tcttcggtga tgattttgtc ggtgacgaga tggacatcgg gaaacatttt
     4921 tctgaattcg tcagccgcgc gccagtgcgt cgagcatttt ctgcccgtta aaagcccggt
     4981 tttcgccagc agaaacgcgc cgacgcagag gctgacgact tccgcgccat tttcgtattg
     5041 ctcgacaatc cacggaataa atttt
//
LOCUS       DASWNE010000378         2929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_189126, whole genome shotgun sequence.
ACCESSION   DASWNE010000378 DASWNE010000000
VERSION     DASWNE010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189126"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..628)
                     /locus_tag="VGB68_07595"
     CDS             complement(104..628)
                     /locus_tag="VGB68_07595"
                     /inference="COORDINATES: protein motif:HMM:NF025675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HEY0049132.1"
                     /translation="MPDDGAILEIEYELDADFVRRAWFDQFRKGLPSVLAFWGTIIFI
                     GLLAMIYYRQSAREFVFFSLVVGIVVAVLSYIYYSSYSQSMRETRELNSVAGKNVRLV
                     FKSDADGFDYINGKNFSHIAWDSVSFYEESGEFIILGYSGGFYIPKSAFRSDAEISFL
                     KSLVQIKSLAPRGK"
     gene            complement(633..1832)
                     /locus_tag="VGB68_07600"
     CDS             complement(633..1832)
                     /locus_tag="VGB68_07600"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY0049133.1"
                     /translation="MKKFLFITFLAFALFLSGCRTPPGERNHANHQQNAKQAEIDMSA
                     EDFAVEFKAAPDQPKAEEQVDLSFVVKNSSGGIIKDLQIVHEKPMHLIVVSEDLAEFY
                     HLHPEAQADGSYKTSFAFKNGGKFKLYADLSPVKDRQLVRIFDLNVAGNERAKEELKT
                     DAKFEKTLEDLRVVMKPDGEFAANKQLMLAFNVFDARTSQPVNDLQKYLGETAHFVIV
                     NKDLNEFVHAHPMSADNVKQDGAHAHDGSSATKHDDKLMSAAANSVAAHVSFPKAGIY
                     KIWAEFQRNGKVTAVPFVVNVKAGAEEKPIEEVKIPDGAVKITVTKDGFAPEEITFKK
                     GQPLKLAFYRADAENCADQIVFKDLNIKKNLPVGEVVVIDVPTDSEKTFTFACGMNMY
                     KGQVVVQ"
     gene            complement(2255..>2929)
                     /gene="fusA"
                     /locus_tag="VGB68_07605"
     CDS             complement(2255..>2929)
                     /gene="fusA"
                     /locus_tag="VGB68_07605"
                     /inference="COORDINATES: protein motif:HMM:NF015706.4"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY0049134.1"
                     /translation="VPYKETITSQVEVQGRHKKQSGGRGQFGDCKCIFEPLERSGGFE
                     FVDKIFGGAIPQNFRPAIEKGIVEAAQSGAVAGYPLVDFKVTLIDGSYHAVDSDEHSF
                     RAAGRKAFRNAMEKARPTLLEPVMDVEVFCPQEVSGDIMGDLNSRRGRVQGMETRGKQ
                     QVIKAQVPLSEMLDYQSKLNSVTQARGSYHMQFSHYDPLPGNLASKVIEQARAEGRIR
                     THEEDE"
BASE COUNT          751 a          746 c          661 g          771 t
ORIGIN      
        1 ttttgcggat tgcgcgaagg tttcagcaat ccgatgcgcg tgtagtagcg aatcgtgtca
       61 atcggaacgg cggcttgttt ggcgagcgta ttgacggtaa tcatcatttt cctctcggag
      121 cgagcgattt tatctgcaca aggcttttta aaaaactaat ttcggcgtcg cttcgaaaag
      181 cgcttttggg aatataaaag ccgccggagt agccgagaat aataaattcg ccggattctt
      241 cgtaaaacga gactgaatcc cacgctatgt gactaaaatt tttcccgttt atataatcaa
      301 aaccgtcggc gtcggatttg aaaaccaatc ttacgttttt accggcgacc gagttgagct
      361 ctcgcgtctc gcgcattgac tggctgtagc tcgaataata aatatacgag agaacggcga
      421 cgacgattcc gacgaccagg gagaaaaaaa caaattcgcg cgccgattgg cggtaataaa
      481 tcatcgccag cagtccgata aaaattattg ttccccaaaa ggcgagaacg ctcggtaaac
      541 ctttgcgaaa ctggtcaaac cacgcgcgcc ggacaaaatc cgcatccaac tcgtattcaa
      601 tttcgagtat cgctccgtcg tccggcataa aattactgga cgacgacctg acctttatac
      661 atattcatcc cgcaggcaaa agtaaaagtt ttttcgctgt cggtcgggac gtcgatcacc
      721 acgacctcgc cgacgggcag attttttttg atgttcaaat ctttgaaaac aatctggtcg
      781 gcgcagtttt ccgcatcggc tcggtaaaaa gccagtttta aaggctgtcc ttttttgaaa
      841 gttatctctt cgggcgcaaa accgtctttc gtcaccgtaa ttttaaccgc gccgtcggga
      901 attttgactt cttcgattgg cttttcttcc gcgcccgctt tcacgttgac gacaaacgga
      961 acggcggtca ctttcccgtt gcgctgaaat tccgcccaga ttttataaat tccggctttc
     1021 ggaaacgaaa cgtgcgccgc aaccgaattt gccgccgcgc tcatcaattt atcgtcgtgt
     1081 ttcgtcgcgg aactgccgtc gtgcgcgtgc gcgccgtcct gtttcacatt gtccgccgac
     1141 atcggatgcg cgtggacaaa ctcgttcaaa tctttattga cgatgacaaa atgcgccgtc
     1201 tcgcccagat atttctgcaa atcgttaacg ggctggctcg tccgcgcgtc aaagacatta
     1261 aaggcgagca tcaattgctt gtttgcggcg aattcgccgt ccggcttcat cacgacgcgc
     1321 aaatcttcca acgttttttc aaatttcgca tccgttttca attcttcctt cgctcgctcg
     1381 ttgcccgcga cgtttaaatc gaaaatcctg accaactgcc tgtctttaac cggcgataaa
     1441 tcagcgtaaa gcttaaattt cccgccgttt ttaaaagcaa acgaagtttt gtaagaaccg
     1501 tccgcctgcg cttccggatg cagatgataa aactccgcca aatcttccga aacgacaatc
     1561 aaatgcatcg gtttttcgtg gacaatctgc aaatccttga tgattccgcc gctggaattt
     1621 ttgacgacaa agctcaaatc gacctgttct tccgctttcg gctggtcggg cgcggcttta
     1681 aactcgacgg cgaaatcttc cgcgctcata tctatttccg cctgcttggc gttttgttga
     1741 tgattggcgt gattccgctc gcccggcggc gtccggcatc cgcttaaaaa caatgcgaaa
     1801 gccaaaaatg tgataaataa aaactttttc atccggtttc ctgttttgct gaatagagtt
     1861 atcgaaatac agacagaaac gtatcaaatt caaaacgcaa aatgcaaggt aggattcaga
     1921 attcaagatt gccgattcaa attccgactt ccaaatccca atttagatat tttagtcggc
     1981 aaaagaaaaa gcggcgttgt attcaaactc aacgccgcta atcattccac aaggagaaac
     2041 aaaatacctg acagatagac actgataaat taaacagaaa ttcgctaaat gtcaagaaaa
     2101 atttgaagaa aactaaaaac taaaataaat gaaacgctga ttcgcgaaaa aaaatcagaa
     2161 atttacaaat ttggaattgt agttatttaa ttggaattga aaaaggacgg cgcgctgaac
     2221 cgtcctttaa atttaatatt tgaattattg acgcttactc gtcttcttcg tgcgttctga
     2281 ttctgccttc ggcgcgcgcc tgttcgatga ctttcgaggc gaggttgccg ggcagcgggt
     2341 cgtaatgcga aaactgcata tgatacgagc cgcgcgcctg cgtcaccgaa ttcagcttcg
     2401 attgataatc gagcatttcc gagagcggaa cctgcgcttt tatcacttgc tgtttgccgc
     2461 gcgtctccat tccctggacg cgaccgcgac gcgagtttaa gtcgcccata atgtcgcccg
     2521 aaacttcctg cgggcagaaa acttcgacgt ccattaccgg ttcgagcaaa gtcggacgcg
     2581 ctttttccat cgcattacgg aaagctttgc gcccggcggc gcggaatgaa tgctcgtccg
     2641 aatcgaccgc gtgataactt ccgtcaatca gcgtgacttt gaaatcgacc agcgggtaac
     2701 cggcgaccgc gccgctttgc gccgcttcga cgatgccttt ttcaatcgcc ggacggaagt
     2761 tttgcggaat cgcgccgccg aagattttat cgacaaattc aaaaccgccg cttcgttcca
     2821 gcggttcgaa gatacatttg cagtcgccga attgtccgcg cccgccggat tgttttttat
     2881 gccgaccctg aacttcgacc tgcgacgtaa tcgtttcctt gtagggaac
//
LOCUS       DASWNE010000379         3047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_364501, whole genome shotgun sequence.
ACCESSION   DASWNE010000379 DASWNE010000000
VERSION     DASWNE010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364501"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..1466)
                     /gene="pyk"
                     /locus_tag="VGB68_07610"
     CDS             complement(18..1466)
                     /gene="pyk"
                     /locus_tag="VGB68_07610"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEY0049135.1"
                     /translation="MRRAKILATLGPASNSPEILELLLLAGLNAVRINMSHGDQSEHA
                     ETIRRVRETAKKLDKPLSVLVDLSGPKIRTRKLAGGMPVFLEDGARFTITARDIVGNA
                     QEVSTTFMELPKLVQPYTRILLDDGAIELEVESAAGDDVVTRVVSGGWLSERKGINLP
                     GTILPIPSLTDKDRVDLEWAMRQNIDYIALSFVRRAEDCIEVKELIKQLNKRETGRPL
                     LVAKIEKAEAIENLDSILAVTDGVMVARGDLGVETSTELVPVYQKTIIEKAVKADRFV
                     ITATQMLQSMIAEPRPTRAEASDVANAVWDGTDAVMLSGETATGKYPVEAVATMARII
                     NSAETVKTDKLRQPVKFGEEPTGRTSQALCKAAAICAREILTDKVAVFTESGLMARRL
                     SSVRSGLQTFALTNSDNVRNQLSLIWGVEPFCHEDKKTTEHMLKEGEDTLLKAGVVNQ
                     GETIVMMAGRLSGLGLSSSVIVWTIGENVPRR"
     gene            complement(1530..2042)
                     /locus_tag="VGB68_07615"
     CDS             complement(1530..2042)
                     /locus_tag="VGB68_07615"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY0049136.1"
                     /translation="MEKYQTLIPRFCALILDAVLLLPLAIIEELIKSDEFSQSAKWVL
                     FFIVGLAETMYFVVMHGLFGQTVGKMLMKIKVLDISETPLKFRQAILRDLLQLLLVAG
                     SFIFLYPSAPNEIEPNSPGYWKNPFFVLILIWGVADLVVFFTNEKRRALHDYIARTVV
                     VRINENSDAE"
     gene            complement(2042..>3047)
                     /locus_tag="VGB68_07620"
     CDS             complement(2042..>3047)
                     /locus_tag="VGB68_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEY0049137.1"
                     /translation="ANALDLAGYPSLTRKFYELCGKIIHENGYFLQKYNADGTVASGW
                     HAAWDVWAKKRLVPIQEDETALVVWALWQHYDRYRDIEFVRRLYRPLILKAANFMCMF
                     RDAETRLPLPSWNLWEDRRGIHTFTCSTVVGGLRAAARFAYLFGEAEIAQKYEASANE
                     IVRAMREHLYSARLGRFLRALQFHNDDNFETDATVDASLFGTFYFGAFEADDELVKNT
                     MQAVREHLWAKTEIGGAARYQNDDYMRVTGQATGNPWFICTLWLADFYIAEARGRADL
                     DKALEILEWTVSRALPSGVLAEQVNPVDGAPVSVSPLTWSHSTFVATVMNYLNKLKVI
                     "
BASE COUNT          688 a          886 c          783 g          690 t
ORIGIN      
        1 cggcgtttcc ttcaaatcta tcttcgcggc acgttttcgc cgattgtcca gacgatgacc
       61 gagctcgata aacccaagcc cgacaatctg ccagccatca tcacaatcgt ctcgccctga
      121 ttgacgactc cggctttgag caaagtgtct tcgccttctt tcagcatatg ctcggtcgtt
      181 tttttatctt cgtgacaaaa cggctcgacg ccccagatca gcgaaagctg gttgcggacg
      241 ttgtcggaat tggtcagcgc aaaggtctgc aaaccggagc gaacgctgga aagacgacgc
      301 gccatcagcc cggactcggt aaagacggcg actttatccg tcagaatctc gcgcgcgcaa
      361 atcgccgccg ctttgcagag cgcctgcgag gttctgcccg tcggttcttc gccgaatttt
      421 accggctggc gcagcttgtc ggttttcacg gtttccgccg agttgataat gcgcgccatc
      481 gtcgccaccg cttcgaccgg atattttccg gtcgccgttt cgcccgagag catcacggcg
      541 tcggttccgt cccagacggc gttcgccacg tccgaagctt cggcgcgcgt cgggcgcggc
      601 tcggctatca tcgattgcag catctgcgtc gccgtgatga cgaaacggtc ggctttgacc
      661 gctttttcga taatcgtttt ctgatagacg ggaaccagct cggtgctggt ctcgacgccc
      721 aaatcgccgc gcgcgaccat tacgccgtcg gtcaccgcca gaatcgagtc gagattttca
      781 atcgcttccg ccttttcgat tttggcgacg agcagcggtc tgcccgtctc gcgcttgttc
      841 aattgtttga taagctcttt aacctcgatg cagtcttcgg ctcggcggac aaacgagagc
      901 gcgatgtaat cgatattctg ccgcatcgcc cattccaaat cgacgcggtc tttgtccgtc
      961 aggctcggaa tcggcaaaat cgtgccgggc aggttgatgc ccttgcgctc cgagagccag
     1021 ccgccgctga cgacgcgcgt cacgacatcg tcgcccgcgg cgctttcgac ttccagctca
     1081 attgcgccgt cgtcgagcaa aatccgcgtg tagggctgga cgagcttcgg caattccata
     1141 aaagtggtcg aaacttcctg cgcgttgccg acgatgtcgc gggcggtaat cgtaaatcgc
     1201 gcgccgtctt cgagaaaaac cggcattccg cccgccagtt tgcgcgtgcg gattttcggt
     1261 cccgaaaggt cgaccagaac cgacaaaggc ttgtccagtt tcttcgccgt ttctctgacg
     1321 cgccggatgg tctcggcgtg ctcgctctgg tcgccgtgcg acatatttat cctgacggcg
     1381 tttaatccgg caagcagcag cagttccaga atttccggtg aattggaagc cggtccgagg
     1441 gtggctaaaa tttttgctcg tctcattaga ttaagtttaa cgcaaagacg cgggcgacgc
     1501 gaaagagtca gggaaaagtc gccgaaaatt tattccgcgt ccgaattttc atttatcctg
     1561 acgacgaccg tgcgcgcaat ataatcgtgc agcgcgcggc gtttttcgtt tgtgaaaaat
     1621 acaaccaaat cggcgacgcc ccagattaaa attaaaacga aaaaaggatt tttccagtaa
     1681 ccgggcgaat tcggctcaat ctcattcggc gctgacgggt agagaaaaat aaatgaaccg
     1741 gcgaccagca atagctgcag caaatctctg agaatcgcct gccgaaactt taacggcgtt
     1801 tcggaaatgt ctaaaacctt gattttcatc agcatttttc cgaccgtctg cccgaacaaa
     1861 ccgtgcatca ccacgaaata catcgtttcg gctaaaccga caataaaaaa taaaacccat
     1921 ttagccgact gcgaaaattc gtcgctttta attaattcct cgataatagc cagcggcaaa
     1981 agcaaaacgg cgtcgagaat taaagcgcaa aagcgcggta tcagcgtctg gtatttttcc
     2041 attagattac tttaagctta tttaaataat tcatcaccgt cgccacaaaa gtcgaatgcg
     2101 accaggtcag gggcgaaacc gagacgggcg cgccgtcaac cggattaacc tgttccgcca
     2161 gaacgcccga aggcagagcg cggctcacag tccactccag aatctccagc gctttgtcca
     2221 aatccgcgcg cccgcgcgcc tctgcaatat aaaaatcagc taaccataaa gtgcagataa
     2281 accagggatt tcccgtcgcc tgtccggtca cgcgcatata atcgtcgttt tggtagcgcg
     2341 ccgcgccgcc gatttccgtt tttgcccaga gatgctctct gacggcttgc atagtgtttt
     2401 tgacgagctc gtcatccgcc tcgaacgcgc cgaaataaaa cgtgccgaaa agcgatgcgt
     2461 cgacggttgc gtcggtttca aaattatcgt cgttatgaaa ctgcaaagcg cgcagaaaac
     2521 gtccgagccg cgcgctgtaa agatgctcgc gcattgcccg cacgatttca ttcgccgagg
     2581 cttcatactt ttgcgcgatt tcggcttcgc cgaaaagata tgcaaaacga gccgccgcgc
     2641 gcaaaccgcc gaccacggtc gaacaggtaa aagtatgaat tccgcgccgg tcttcccaca
     2701 aattccagct cggcagcggc agcctcgtct ccgcgtcgcg aaacatacac ataaaattcg
     2761 ccgctttcag aatcagcggg cggtaaagcc gccgcacgaa ttcgatgtcg cggtagcggt
     2821 cgtaatgctg ccataaagcc cagacgacca gcgccgtttc gtcttcctga atcggcacga
     2881 gccgtttttt cgcccagacg tcccacgccg cgtgccaccc ggaagcgacc gtgccgtcgg
     2941 cgttgtattt ctgcagaaaa taaccgtttt cgtgaatgat tttcccgcac aattcgtaaa
     3001 atttgcgcgt cagcgaagga taaccggcga ggtcgagcgc gttggcg
//
LOCUS       DASWNE010000380         5142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_471308, whole genome shotgun sequence.
ACCESSION   DASWNE010000380 DASWNE010000000
VERSION     DASWNE010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5142
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471308"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="VGB68_07625"
     CDS             complement(<1..1346)
                     /locus_tag="VGB68_07625"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEY0049138.1"
                     /translation="MDDSFSNILLFFAGDVPAVPEDASSWVSIVLKVVAVLVLVAVNG
                     FFVAAEFAYVGVRRSRIETLAAEGNKSAKRLLNLLNNLNAYLSASQLGITLASLGLGA
                     LGEPVVEAMLGGVLSAYLPESMRHFASYFIAFLIVTCLHIVLGEQAPKLFGLQLAEKV
                     AMGTALPMLIFYKTFKPLIHALDWASAKTVNLFGLHASSEHASIYTEDEIRQLVKISE
                     ESGHLNREERKLINRVFEFSETTVREAMIPRTEIAAISASCGVEEIARAFRQYGYSRL
                     PVYRESLDDIAGVIHSKDVMPFLLRPKVFRLEKVLQKPVYVVDTARLEDVLRQMQKEK
                     FHFGFVVDEHGGVEGIITLEDLLEEIVGDISDEHDEEVNEQIQKIQDGAYLLDGGLAV
                     RDLNRRLGMNVPVSESYTTIAGFLMNESGQILNEGESVKFNGHVFHVEKVDTRRIL"
     gene            complement(1472..2065)
                     /locus_tag="VGB68_07630"
     CDS             complement(1472..2065)
                     /locus_tag="VGB68_07630"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEY0049139.1"
                     /translation="MSFYPEKIGERFRAPRHAGKTSGANAVGTEASFVCGAAVRFTLR
                     IEKETKEILEAKYQTSGCGFLIAAADALAEKIGGKKLIELHGLERAELQGQIENEIGK
                     FPASRNHCVQLCADALQAALADFRAAQLEEFTGEKALICTCFGVSEETIERVAREYSL
                     EIVEEVTEICRAGGGCGSCQPLIQDILENIRFDAPYK"
     gene            2159..2725
                     /locus_tag="VGB68_07635"
     CDS             2159..2725
                     /locus_tag="VGB68_07635"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY0049140.1"
                     /translation="MRVFAFCLLPFAFLFSACRPSAAPVSVSNQPISINGVPQTNLPM
                     PPLKNVENLGWQTFEGKQIKLGEMKGKVTVLDFWATYCPPCLEEIPHLVSLQEQYRAR
                     DLEVVGLHVGGEEDRPKVPEFVGKLKMNYTLATPEDDLTNALFGGNSAIPQTFVFGRD
                     GKLIKKIVGFDAQKKAELDAAIEQAIRN"
     gene            2727..3230
                     /locus_tag="VGB68_07640"
     CDS             2727..3230
                     /locus_tag="VGB68_07640"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY0049141.1"
                     /translation="MKDSNTEELKESNTEKSKPKFPIVEQISAGGVAFRRTGAEIEAA
                     IVAVNPSRRWQLPKGIIDAGETPEIAALREVREEAGIETELLELIEKVEYWYVGFHKG
                     ERVRFHKFVYFYLLRYLRGDVEDHDHEIAEARWTSIPEAIEMLAFKSEKEVVKKAFDK
                     IKTAEEI"
     gene            3374..3781
                     /locus_tag="VGB68_07645"
     CDS             3374..3781
                     /locus_tag="VGB68_07645"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY0049142.1"
                     /translation="MIKLEDARRIIAAAEKKAAEIGQPMNIAVADAGGNLVAHVRMDG
                     AWIGSIDISIKKAWTSRAFDIATGDLAEHSQSGDQFFGIHASNNGRVMIFAGGIPLKK
                     GGQVAGAIGVSGGSGEQDHSVAEAGAAAFEGSE"
     gene            complement(3937..>5142)
                     /locus_tag="VGB68_07650"
     CDS             complement(3937..>5142)
                     /locus_tag="VGB68_07650"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0049143.1"
                     /translation="LEFGEELKVDAVLEGTLQTIENRLRVNLRLWDVRDGAQLWQNSF
                     DSAEADFFNLQDAVSTKVTQSLVSQLIEKDRQLLTRHDTENRDAFDAYWRGRFFLEKR
                     NTERAIAEFEQAINFDPKYALAYTGLADAYIWQANFTSGSDAELYAKAKIATDQALEL
                     DPNLAEAHSSLGRIKYNYDWDWKGAEESFRQAIKLNPDSVNAHQFYARLLTTLGRYDE
                     GLAEINKARQLDPRSADLGVPLFAILEKRGEFDQSLKILEATLEMDGDSQFARRGIGK
                     IHLLKGDYAKVIESGNRLFPNPKDADFAWASMLATAYYKTGQTEKATEMRNHLKKMAE
                     KDTKSLYFLALHDSELGRRDEALDALQKCFELREERLIWTKDEPRFANLKDDSRFQEI
                     LRKMNLDIK"
BASE COUNT         1238 a         1365 c         1327 g         1212 t
ORIGIN      
        1 cttaaaattc gtcgcgtatc tactttttcg acgtggaaaa cgtgaccgtt gaatttcacg
       61 ctttcgcctt cgtttaaaat ctgtcccgat tcgttcatca gaaatccggc gatcgtcgtg
      121 tagctttccg aaaccggcac gttcataccc agacgacgat tcaaatccct gaccgccaga
      181 ccgccgtcca aaagatacgc gccgtcctga attttctgaa tctgctcgtt gacttcctcg
      241 tcgtgctcgt cggaaatatc gccgacgatt tcttccagaa ggtcttccag agtgatgatt
      301 ccctcgacgc cgccgtgctc gtcgacgaca aagccgaaat gaaatttttc cttctgcatc
      361 tggcgcagaa cgtcttcgag ccgcgccgta tcgacgacgt aaaccggctt ctgcaaaact
      421 ttttcgagcc ggaaaacctt cggtcgaagc agaaaaggca tcacgtcttt cgagtgaatg
      481 acgccggcga tgtcgtccag cgattcgcga taaaccggca ggcgcgaata accgtattga
      541 cgaaaagcgc gcgcgatttc ttcaactccg caagacgccg aaatcgcggc gatttccgtg
      601 cgcggaatca tcgcctcgcg cacggtcgtc tcggaaaatt cgaaaacgcg attgattaat
      661 tttcgctctt cgcgattcaa atgcccgctt tcctcggaga ttttgacgag ctggcggatt
      721 tcgtcttccg tgtaaatcga agcgtgctcg ctcgaagcgt gcaagccgaa aagattgacg
      781 gttttcgcgc tcgcccaatc cagcgcgtgg ataagaggct tgaaagtttt atagaaaatc
      841 agcatcggca gcgccgttcc catcgccact ttttcggcta gctgcaaacc gaacagcttc
      901 ggcgcctgct cgcccagaac gatgtgcaga cacgtgacga tgagaaaggc gataaaatag
      961 gaagcgaaat gtcgcatcga ttccggcaga taagcgctca aaacgccgcc gagcatcgcc
     1021 tcgacgaccg gttcgccgag cgcgcccaaa cccagtgaag cgagcgtgat gccgagctgc
     1081 gaggcggaca gataagcgtt cagattattt aacagattga gcagccgttt agcgcttttg
     1141 ttgccctcgg cggcgagcgt ttcgatgcgc gagcggcgaa cgccgacata agcaaactcc
     1201 gccgcgacga aaaagccgtt gaccgcgacc agaaccagca ccgccacgac ttttaaaacg
     1261 attgaaaccc aggaagatgc gtcttcgggc acggcaggaa catcgcccgc aaagaaaagc
     1321 agaatattac taaaagaatc gtccattgct aaaaaatccg cggatttaaa tcaaaaaaat
     1381 caacttcaaa aaaatatttt tcgattataa cactttcgga cagttgatgt taagactttc
     1441 tatattttga cggtcgctta atcactgcgc gctacttata cggcgcgtcg aaccgtatgt
     1501 tttccagaat gtcctgaatc aaaggctggc acgagccgca gccgccgccc gcgcggcaaa
     1561 tctcggtgac ttcctcgaca atttccagcg aatattcgcg cgcgacgcgc tcgatcgttt
     1621 cttcggaaac gccgaaacag gtgcagatga gcgccttttc gccggtgaat tcttcgagct
     1681 gcgcggcgcg aaaatccgct aaagccgcct gcaaagcgtc agcgcataat tgaacgcaat
     1741 gattgcgaga agcgggaaat ttaccgattt cattttcgat ttgtccctgc agctccgcgc
     1801 gctccagccc gtgaagctcg attaattttt tcccgccgat tttttccgcc agcgcgtccg
     1861 ccgccgcgat taaaaagccg cagccgctgg tttgatattt cgcttcgaga atttcttttg
     1921 tttccttttc aatccgcagg gtaaagcgca ccgccgcgcc gcagacgaaa ctcgcctccg
     1981 tcccgacggc gtttgcgccg gaggttttgc ccgcgtgtcg cggcgcgcga aatctttcgc
     2041 cgattttttc gggataaaaa ctcactggtc aatcgtaaat cgtcagtcgt taatagtaaa
     2101 attctttttt gagcctttca tttacaattt gcagtttagg atttacgatt tactttttat
     2161 gagagttttt gccttttgcc ttttgccgtt cgccttcctg ttctcggcgt gtcgcccgtc
     2221 cgccgcgccg gtttcggttt ccaatcaacc gatttccata aacggcgttc cgcagacgaa
     2281 tctgccgatg ccaccgctga aaaacgtgga aaacctcggc tggcagacgt ttgaaggcaa
     2341 acaaataaag ctcggcgaaa tgaaaggcaa agtgacggtg ctggatttct gggcgacgta
     2401 ttgcccgccg tgtctggaag aaattccgca tctggtcagc cttcaggaac aatacagggc
     2461 gcgggatttg gaagtcgtcg gcttgcacgt cggcggcgag gaagaccgcc cgaaagttcc
     2521 ggaatttgtc ggaaaattga aaatgaatta cacgctcgcc actcccgaag atgatttaac
     2581 caacgctttg ttcggcggca actcggcgat tccgcaaact ttcgttttcg ggcgcgacgg
     2641 aaaattgata aagaaaatcg tcggcttcga cgcgcaaaaa aaagccgagc tggacgcggc
     2701 gattgaacag gcaatcagaa attaatttga aagattcgaa cacggaagaa ttgaaagaat
     2761 caaacacgga aaaatcgaaa ccgaaattcc cgattgtcga gcaaatctcg gcgggcggcg
     2821 ttgcttttcg ccgaaccggc gcggaaatcg aagcggcaat cgtggcagtc aatccgagcc
     2881 gtcgctggca gctgccgaaa ggcattatcg acgcgggcga gacgccggaa atcgccgcgt
     2941 tgcgcgaagt gcgcgaggaa gccggcatcg aaaccgagct gctggaattg attgaaaaag
     3001 tcgaatactg gtacgtcggt tttcacaagg gcgagcgcgt ccgctttcac aaattcgtct
     3061 atttttatct gctcagatac ctgcgcggcg acgtcgaaga ccacgaccac gaaatcgccg
     3121 aagcgcgctg gacgagcatc cctgaagcga ttgaaatgct ggcttttaag agcgagaagg
     3181 aagtcgtcaa aaaagcattc gacaaaatca aaacggcgga agaaatttga aatccgagat
     3241 taatcgccgg ctaaaattcc ggctggcgat ttcaaagctc aaataaaatg taaaaagggc
     3301 gagcttgttc gctcttttgc ttgtgcgtta ctataaacac tcaaacaaaa ataatttaaa
     3361 gagggttaaa tttatgatta agctggaaga cgcgcgccgg attatcgccg ccgccgaaaa
     3421 gaaagccgct gaaatcggtc agccgatgaa catcgccgtc gccgacgcgg gcggaaatct
     3481 cgtcgcgcac gttcggatgg acggcgcgtg gattggcagt attgatattt ccatcaaaaa
     3541 ggcgtggacg tcgcgcgctt ttgacatcgc caccggggat ttggcggagc acagccagtc
     3601 gggcgaccaa tttttcggca ttcacgcttc gaataacgga cgagtgatga tttttgccgg
     3661 cggcattccg ctcaaaaagg gcggtcaggt ggctggcgct atcggcgttt cgggcggaag
     3721 cggcgagcag gaccactcgg ttgccgaagc gggcgcggcg gcttttgaag gctcggagta
     3781 aaatacgaaa atcgagttaa cgcttcgtgt cgaagaaaaa cgggacgctg attttttagg
     3841 atttatcaga attagttaag ataaattcaa ataaaaacgg cgttcttttt ttcgaagccg
     3901 tgcgtccccg cttgccgccc gcgcgggctt ccgcctttat ttaatgtcga gattcatttt
     3961 cctcaggatt tcctgaaaac gcgaatcgtc tttcaaatta gcaaaacgcg gctcgtcttt
     4021 tgtccagatt agccgttcct cgcgaagctc gaagcatttt tgcaaagcgt ctagggcttc
     4081 gtcccggcgt ccgagctcgc tgtcgtgcag cgccagaaaa tacagggatt tcgtatcctt
     4141 ttcagccatt tttttcagat gattccgcat ttccgtcgct ttttccgttt gtccggtttt
     4201 gtaataagcg gtcgccagca tcgacgccca cgcaaaatcg gcgtctttcg gattaggaaa
     4261 cagccggttg cccgattcga tgacttttgc ataatctcct ttgagcaggt ggattttgcc
     4321 gatgccgcgc cgggcaaact gcgaatctcc gtccatttcc agagtcgcct ccagaatttt
     4381 cagagactgg tcgaattcgc cgcgtttttc cagaatggcg aaaagcggca cgcccaaatc
     4441 agccgaccgc gggtcgagct gccgcgcttt attgatttcg gcgagccctt cgtcgtagcg
     4501 tccgagcgtc gtcagcaggc gcgcgtaaaa ctgatgcgca ttgacgctgt ccggattaag
     4561 cttaatcgcc tgccggaaag attcttccgc gcccttccaa tcccaatcgt agttgtattt
     4621 aatccgcccg agcgagctgt gcgcctcggc gagattcggg tctaattcca gcgcctggtc
     4681 ggtcgcgatt ttggctttgg cgtaaagctc ggcgtccgag ccgctcgtaa aattcgcctg
     4741 ccagatataa gcgtccgcca aacccgtgta agccagagca tatttcgggt cgaaattaat
     4801 cgcctgctcg aattcggcaa tcgctctttc ggtatttcgt ttttccagaa aaaaacgtcc
     4861 gcgccagtag gcgtcgaaag catcgcggtt ttccgtgtcg tgtctggtta aaagctgccg
     4921 gtctttttcg attaactgcg aaaccagcga ttgcgtgact ttggtcgaga cggcgtcctg
     4981 aagattgaaa aaatcggctt ccgccgaatc gaacgagttt tgccagagct gcgcgccgtc
     5041 gcgcacgtcc cacaaacgca ggttgacccg caggcgattc tcaatcgtct gcaaagtgcc
     5101 ttccaaaacg gcgtcgacct ttaattcctc gccgaattca ag
//
LOCUS       DASWNE010000381         3995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_647811, whole genome shotgun sequence.
ACCESSION   DASWNE010000381 DASWNE010000000
VERSION     DASWNE010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647811"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..665)
                     /locus_tag="VGB68_07655"
     CDS             complement(180..665)
                     /locus_tag="VGB68_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY0049144.1"
                     /translation="MYCSTCGTAINNGLNYCKNCGARASGAKTEDDGKLSESSFNLLV
                     VAVLGIPIAGLGIIIGLMSVMKKELGFSNELITIFISASFLLLLFSEAVLIWLYIQRT
                     RGIRETDDNSRLKDDAQLPEVLIKGLDPAKTRDLIEPIPSVVEDTTRSLEPIARKSKL
                     P"
     gene            complement(746..1441)
                     /locus_tag="VGB68_07660"
     CDS             complement(746..1441)
                     /locus_tag="VGB68_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049145.1"
                     /translation="MDGRTQQARETSRAAQTEICRKRFLRFFPKGFHDPKYIDWERGY
                     KWTAHEQWNEVLNQKAFRSLIKKGEFAAIAAHAVRIESKTNLLFSFEKMALRDAVKTV
                     DGARDFAVGLYEFLYGAGKPANKFENWSATLASLPRRRTRVLTWTTATIFPFIAQPDQ
                     HIFLKPNTMRSAARQYGFDFQYKSSPAWEIYANLLEFAETIRRDQSDLRPRDMIDLQS
                     FIWVIGSDEYARM"
     gene            complement(1551..1778)
                     /locus_tag="VGB68_07665"
     CDS             complement(1551..1778)
                     /locus_tag="VGB68_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049146.1"
                     /translation="MRIIFNLLVVFASLLSIFILALLVSALIYGEFSILLPGLGLIIN
                     FLGIIIPLLIIDALIIAAALLIRLLVLKKSS"
     gene            complement(1915..2451)
                     /locus_tag="VGB68_07670"
     CDS             complement(1915..2451)
                     /locus_tag="VGB68_07670"
                     /inference="COORDINATES: protein motif:HMM:NF022461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2846 domain-containing protein"
                     /protein_id="HEY0049147.1"
                     /translation="MFRNLSFALLFVFVCALASFAQEAQSTPEKQISAKDKQKELRLK
                     ACGTEEVNYDAKTDKTDRPLPEQPADKALIYVVRTAIIGYKIHSKLAVDGKWMGVNRG
                     ATHFYFTLDAGEHFFCSESENQDYLALKIEAGKTYYLKQQVEMGMFKARTNLTVLNEE
                     EGKKAVAKTNLSVFELKK"
     gene            3011..3865
                     /locus_tag="VGB68_07675"
     CDS             3011..3865
                     /locus_tag="VGB68_07675"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HEY0049148.1"
                     /translation="MDNRIHREIIGGHLLVIGGAEDKYNERRILRKFLELAGGEGANI
                     LIVPVSSDFPEFAADVYVQAFRRLGVANPRVLRATSRQDVVSADADAVLDGIDGVFMT
                     GGDQMRLTSVLGGTLFAQKLRQKVAETDLVLAGTSAGAAAMSTAMIVRGDATSHPHKN
                     SVRISPGLGFLKNIIIDQHFTERGRISRLITAVSYNPYNLGIGIDENTAIILNREGML
                     EVFGAGTATIVDGSQITYNEIAEVDDFQPFSVTGVQIHILRDGLIYDYFGRSPLQPPN
                     EFLLPDIE"
BASE COUNT         1017 a          953 c          957 g         1068 t
ORIGIN      
        1 aaaatttacg acgccgtcca cagataaagg gatttatgtt tttactttat tatctaaatt
       61 tacggcttgg agttttattc ggaattcgca agacttttta tccgctgagc ataaaatggc
      121 ggtggaagca ctggaagcaa agagaaattc accggattta ctggtaattc gctaaactct
      181 caaggcagct tcgattttcg ggctatcggt tccagagagc gggtcgtgtc ttcgacgact
      241 gacggtatcg gctcgattag gtcgcgcgtt ttggcagggt caagtccttt tattaaaact
      301 tccggcaatt gagcgtcgtc ctttaaacgc gaattatcgt cggtttcacg gattcctctt
      361 gtgcgttgta tatacagcca gataagtacg gcttcggaga agagaagcag caaaaagctc
      421 gccgaaataa atatggtaat cagctcgttg ctgaaaccga gctccttttt cataaccgac
      481 atcagcccga tgataattcc gagaccggca atcgggattc caagaacagc caccaccaga
      541 agattgaatg acgactcgga caatttgccg tcgtcctccg tttttgcgcc gctggctcgc
      601 gcgccgcaat tcttacagta attcaagccg ttattgatag ccgtcccgca ggttgaacaa
      661 tacatttatt ttacctccga gagctatacg atgctttaag cctcaaagtt cactattaag
      721 tcctcgaact gcctgcgcgg attaactaca ttctggcgta ttcgtcggaa ccgatgaccc
      781 atataaacga ctgcaaatca atcatatcgc gcgggcgcaa atcgctctgg tcgcggcgga
      841 tggtttcggc aaattccagc agatttgcgt agatttccca agccggactc gatttgtatt
      901 gaaaatcgaa gccgtattgg cgggcggcgc tgcgcatcgt gttcggcttt agaaaaatat
      961 gctggtcggg ctgcgcaata aacggaaaaa tcgtcgccgt cgtccacgtc aaaacgcgtg
     1021 tccggcggcg cggaagcgag gcgagcgtcg cgctccagtt ctcgaatttg ttcgccggtt
     1081 tgcccgcgcc gtaaagaaat tcgtagagcc cgacggcaaa atcgcgcgct ccgtctacgg
     1141 ttttaacggc gtcgcgcaga gccatcttct caaacgaaaa aagaaggttc gtttttgatt
     1201 cgatgcggac ggcgtgcgcg gcaatcgcgg caaactcgcc tttttttatc agcgagcgaa
     1261 aagccttttg atttaaaacc tcgttccatt gctcgtgcgc cgtccatttg tagccgcgtt
     1321 cccaatcgat atatttcggg tcgtgaaatc ctttcggaaa aaatcgtaaa aatcttttcc
     1381 ggcaaatttc cgtctgcgcc gcccgtgatg tttcgcgcgc ctgctgcgtt ctgccgtcca
     1441 ttgctgaaaa cctccccgga taatgcgaat tttccttata aatgcgagtt tataacaacg
     1501 cttcgccgcc gtcacgaaaa atttttacgc tgcgtgagag cgcaggttga ttaactggat
     1561 tttttcaaaa cgaggagccg gattaaaagg gcagccgcaa taatcaaagc gtcgataatc
     1621 aacagcggaa tgatgattcc caaaaaattg ataatcaatc ccaagccggg caggagaata
     1681 ctaaattcgc cgtagatgag cgccgacacg agcagcgcga gaatgaaaat cgaaagcaga
     1741 gaagcaaaga cgaccagtaa attaaatatt atgcgcatag gggaaaagaa ggtggaaaag
     1801 cgaaaaagcg gaaaagtgaa aaagcgaaaa agcgaaaaag cgaaaaagaa acggttgttt
     1861 ctcaagcgca agctttttcg ccttttcacg ctttcacttt ttcgcttttg aaatttattt
     1921 ctttaattca aaaacgctca gattggtctt ggcgacggct tttttgcctt cttcttcatt
     1981 tagaacggtc aggttcgtgc gggctttgaa cattcccatt tcgacctgct gtttgagata
     2041 ataagttttt ccggcttcga tctttaaggc gagataatcc tgattttccg attccgaaca
     2101 aaagaaatgc tcgcccgcgt cgagcgtgaa ataaaaatgg gtcgcgccgc gattaacgcc
     2161 catccatttg ccgtcgaccg ccagcttcga gtgaatttta taaccgataa tcgccgtccg
     2221 gacaacgtaa atcagggctt tgtcggcggg ctgctccggc agcggtcggt cggttttgtc
     2281 ggtcttcgcg tcgtaattta cttcttccgt gccgcacgcc ttcaaacgca attccttctg
     2341 cttgtcttta gcggaaatct gtttttccgg cgtgctttgc gcctcctggg caaacgacgc
     2401 taaagcgcaa acgaatacaa acaataatgc aaaacttaaa tttcgaaaca tctttaaatt
     2461 ttcctttttt cttttaaaat tatttttggt tggatttgaa cgtctttttt attgactgga
     2521 agtttgatgc aaatttttgt cttttcggtt ataaatttat ctttttctaa aagatacagc
     2581 gcgcgaacat ccaatatgcc tttcctcctg cgcctcggct aaactctgca aacttattct
     2641 gttcttcaaa attccagttc gtaatttgtt ccctgctgct cctgatagtc tgaaatgccg
     2701 attcccgcaa aaaaatatcg gaatcttata ttcgagtgaa taaaacggcg ctgcaccgca
     2761 attttatacg ctacatcaaa acgtaaagtc aacaatcgtt tgactttcta tggatttttc
     2821 ggacggggcg cgcaattgcg cctgaaattt aaatataacc ggctgattaa aatttatttc
     2881 cactcataat cttgacgcga gggcgcgctt tgcaataact taacttaaag ctttatcgat
     2941 tccgcattcc agattttaag attcgattcc agatttggaa tgcggaattt ttaatttgaa
     3001 ctgaaaataa atggacaatc gcattcatcg agagataatc ggcgggcatc tgctggttat
     3061 cggcggcgcc gaagacaagt ataacgagcg gcgcattttg cggaaatttc tggagctggc
     3121 gggcggcgaa ggcgcaaata ttttaatcgt tccggtgtcg tcggattttc ccgaatttgc
     3181 ggcggacgtt tacgtgcagg cgtttcggcg tctgggcgtg gcgaatccgc gcgttttgcg
     3241 ggcgacttcg cgtcaggacg tagtttcggc tgacgcggac gccgttctgg acggcatcga
     3301 cggcgttttt atgacgggcg gcgaccagat gcgcctgacc tcggttctgg gcggcacttt
     3361 atttgcccag aaactgcgcc agaaagtcgc cgaaaccgac ctcgttctgg ctggcacgag
     3421 cgccggcgcg gcggcgatgt cgacggcgat gatcgtgcgc ggcgacgcga cttcgcaccc
     3481 gcacaaaaat tccgtgcgca tctcgcccgg cttgggcttt ctgaaaaaca tcatcatcga
     3541 ccagcatttc accgagcgcg ggcgcatcag ccgcctgatt accgccgtca gctataaccc
     3601 ctataatctc ggcatcggga ttgacgaaaa cacggcgatt attttgaacc gggaaggaat
     3661 gctcgaagtt ttcggcgcgg gcacggcgac gattgtagac ggctcgcaga taacctataa
     3721 cgagattgcc gaagtcgatg attttcagcc gttttcggtg accggcgtgc agattcatat
     3781 tttgcgcgac ggcttgattt acgattattt cgggcgctcg cctttgcagc cgccaaacga
     3841 gtttctgctg cccgacatcg agtaagcctt tcgcaaataa ttaaagaaat gtaattttcc
     3901 gaacacttaa aaaaacgcgt ttattataaa atcgaaagat aaaactttat gaaaattctt
     3961 gaaatcagaa cactgcgcgg cgcaaattac tggag
//
LOCUS       DASWNE010000382         2929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_437166, whole genome shotgun sequence.
ACCESSION   DASWNE010000382 DASWNE010000000
VERSION     DASWNE010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437166"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VGB68_07680"
     CDS             <1..613
                     /locus_tag="VGB68_07680"
                     /inference="COORDINATES: protein motif:HMM:NF017853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HEY0049149.1"
                     /translation="LGNQQGSEAVEQISRNVGAEPSAVNSAIQMALPAILGGLANNAA
                     TPEGAQSLNNALEQDHDGSLLNNLGGLGSLIFGGANQQQQQQQTAPPRQADAGGILGH
                     VFGNNQGQVAQQISNQSGLNSGQVAQILMMLAPIVMSYLGRQKQQQGLDAGGLSNWLG
                     GQQQQIQKSPQGGFLESMLDRDGDGSSMDDIASMAMNYMTNRR"
     gene            1005..>2929
                     /locus_tag="VGB68_07685"
     CDS             1005..>2929
                     /locus_tag="VGB68_07685"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEY0049150.1"
                     /translation="MNYQPFRHKIFILFFAFLLSLAAIPAGAQPPDPEEYRKLPPVNY
                     IRSRTVDIKHVAIDLRFDWDKEQAFGSTVVTLAPFKDTNKIYLDAAAMTISAVTLAGG
                     APLKFTYDAKKEDDNLEIALDRIVKRGEDVSIKVEYRTNYVNTADAETAIGSFGRGLR
                     FIKPTDDKPNKPRQIWSQGETEFNRYWFPSYDAPNDFRTSELRATVAKPFTVVSNGRL
                     IEVRENTDGTRTFYWKMDIPYTNYLTSIVVGEYTEVKQEWNGIPIINYGYPNESKEVA
                     ATVKNLPDMVKFFSEITGVKYPYPKYAQTFVEDFGGGMENISATTMVEEMIFDERELI
                     DGDSDSLQAHELAHQWFGDYVTTRDWGQIWLNESFATYFDALYNEHSKGRENFLYEKV
                     RGNQQQYFSAWGEGLRRPIVTNRYVNKDAMFDTYAYPRGAAVLHMLRKHLGDEAFFKS
                     LNHYLTSNAHQPVSTEDLRIAIEETTGQSMDWFFDQWLYKMGHPIFEVAKSYDDAKKT
                     LTLSVKQTQKINPKDEFPQVEFFQTNVDIEIDDRVERVWIKPQAENVFTFSVAANPRL
                     VNFDYEGTLIKELKFAKTIDEFLYQAANDKDVLGKRWAMTELGKIAKNPQTPAADKER
                     IMTAFRSAVEKESFWRLR"
BASE COUNT          799 a          759 c          762 g          609 t
ORIGIN      
        1 actcggcaat cagcagggca gcgaagcggt tgagcaaatc agccggaacg tcggcgccga
       61 gccgagcgcc gtaaacagcg ccattcagat ggctttgccc gcaattctgg gaggtctggc
      121 gaataacgcc gcgactccgg aaggagcgca aagcctgaac aacgcgctcg aacaggacca
      181 cgacggcagt cttctgaata atctcggagg gctcggcagc ttgattttcg gcggcgctaa
      241 tcagcagcag cagcagcagc agaccgcacc gccgcggcaa gcggacgcgg gcggaatctt
      301 aggtcacgtt ttcggcaaca atcagggaca agtcgcgcag caaatcagca accagagcgg
      361 tttaaattcc ggacaggtcg cgcagattct gatgatgctc gcgccgattg tgatgagcta
      421 tctcggcagg cagaaacaac aacagggact cgatgcgggc ggcttgtcga attggctcgg
      481 cgggcagcag cagcagattc agaagtcgcc gcagggcgga tttctggaaa gtatgctcga
      541 ccgcgacggc gacggaagct cgatggacga catcgcctcg atggcgatga attatatgac
      601 caaccgcagg taaaaacgaa agacggtaat agacgaaagg cgaaaggcga tagctacaag
      661 gcaggcgagt ttttagatat tccggtaaat attcgaatct aaaaccgccc gccgctttgt
      721 gcctctcgct gctttgcaca aaaataaagg aaatcaacaa aaattccgat ggctaattgt
      781 aaattctcaa ttattaattc taaattactt cacaaaagat aaccgaacat caatttagga
      841 tttacaattt agaatttaca attttccctt tctttatttt tgtgcaaagc cgcctctcgc
      901 ctttcgtctt ccgagcggat tcaccttttg aaacaactcg cgcgcgcttt gcgtatccca
      961 acttgcaatt taattaactc taaaagaaac gcctttttca aaatatgaac taccaaccat
     1021 ttcgccataa aattttcatc ctgtttttcg cttttctgct ctcgctggcg gcgattccag
     1081 ccggcgcgca accgcccgac ccggaagaat accgaaagct tccgccggtc aattacattc
     1141 gcagccgcac ggtcgacatc aaacacgtcg ccatcgattt gcgtttcgat tgggataagg
     1201 aacaagcctt cggctcgacg gtcgtgacgc tcgcgccgtt taaagatacg aataaaattt
     1261 atctggacgc ggcggcgatg acgataagcg cggtcacttt ggcaggcggc gcgccgctca
     1321 aatttaccta cgacgccaaa aaagaagacg ataatctgga aatcgcgctc gaccgcatcg
     1381 tcaagcgcgg cgaggacgtt tcgatcaaag tcgaatatcg gacgaattac gtcaataccg
     1441 ccgacgcgga gacggcaatc ggcagcttcg gtcggggttt aaggtttatc aaaccgaccg
     1501 acgacaagcc gaacaagccg cgccaaatct ggtcgcaggg cgaaacggaa tttaatcgct
     1561 actggtttcc gtcttacgac gcgccgaacg attttcgaac ttccgagctg cgcgccaccg
     1621 tcgccaaacc tttcacggtc gtctccaacg gaagactaat cgaggtcagg gaaaacacgg
     1681 acggcacgcg cacgttttac tggaagatgg acattcccta cacgaattat ctgacttcca
     1741 tcgtcgtcgg cgaatacacg gaagtcaaac aggaatggaa cggaataccg attatcaatt
     1801 acggctatcc gaatgaatcg aaagaggttg ccgcgacggt gaaaaatctg cccgatatgg
     1861 tcaaattctt ttcggaaatt acgggcgtca aatacccgta tccgaaatac gcgcagacgt
     1921 ttgtcgaaga tttcggcggc ggaatggaaa atatttccgc gacgacgatg gtcgaggaaa
     1981 tgattttcga cgagcgcgaa ttgattgacg gcgacagcga cagcctgcag gcgcacgagc
     2041 tggcgcacca gtggttcggc gattacgtga cgacgcgcga ttggggacaa atctggctca
     2101 acgaaagctt cgccacctat ttcgacgcgc tttataacga gcacagcaaa ggtcgcgaaa
     2161 attttcttta tgaaaaagtg cgcggcaacc agcagcagta tttcagcgcg tggggcgaag
     2221 gcttgcgccg cccgattgtc acgaaccgct acgtcaacaa ggacgcgatg ttcgatacat
     2281 acgcttaccc gcgcggcgcg gcggtgctgc atatgctgcg caaacatctc ggcgacgaag
     2341 cgtttttcaa atcgctgaat cattatctga cttcgaacgc gcaccagccg gtttcgaccg
     2401 aagatttgcg tatcgcgatt gaagaaacga ccggtcagtc gatggattgg tttttcgacc
     2461 agtggcttta caaaatggga cacccgattt ttgaagtcgc gaaaagctac gacgacgcga
     2521 aaaagactct gactttaagc gtgaagcaga cgcagaaaat caatccgaaa gacgaatttc
     2581 cgcaggtcga gtttttccag accaatgtcg atatagaaat cgacgaccgc gtcgaaagag
     2641 tctggataaa gccgcaggcg gaaaacgtct ttactttcag cgtcgccgca aatccgcgcc
     2701 tcgtcaattt cgattacgaa ggaactttga ttaaagagct gaaattcgcc aaaacaatcg
     2761 acgaatttct ttatcaggcg gcgaatgata aagacgttct cggcaagcgc tgggcgatga
     2821 cggagctcgg caaaatcgcc aaaaacccgc agacgcccgc cgccgacaag gaaagaatta
     2881 tgacggcttt ccgcagcgcc gtcgaaaagg aatctttctg gcgactgcg
//
LOCUS       DASWNE010000383         2973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_325700, whole genome shotgun sequence.
ACCESSION   DASWNE010000383 DASWNE010000000
VERSION     DASWNE010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325700"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..781
                     /locus_tag="VGB68_07690"
     CDS             80..781
                     /locus_tag="VGB68_07690"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEY0049151.1"
                     /translation="MVRAILMDFNGVIIDDEPLQMKAYAELLKDEGIELTEEGYYSCG
                     GMDDATFLREQFKRANKDIGETKIAELIQKKTSKWREIVTDKVPLFEGAENFIKKAKQ
                     RYAMALVSMANREEINHILDTTGLKDYFDVVVSAENVAACKPNPECYVSAFRQLDAIR
                     SAQGHHPLERRECLVIEDVPQGIQSGKAAGMKTLGVLNTFDEATMRAAGADAIAKNLN
                     DWFPDSIQLVFSKHF"
     gene            926..2425
                     /locus_tag="VGB68_07695"
     CDS             926..2425
                     /locus_tag="VGB68_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049152.1"
                     /translation="MREHLFSIEEAETSLFRTATYLAENVGGGDGHAEAMKEIVAHYI
                     ENGDVDTAALLADTVDDPFSRDRLLLLVAEKCAEIDDDEYALQLVEAIEESGFQSVAR
                     ERLSMRKAAIGEFDKAFELAESLKHSSDAFAVIAAEQSAASLEAESEKTLARIEFPYA
                     KVNALLNIAAGYEAKEEREKAKAAIARAVETARDIEFKEEKIRALQDAATHFAHVSAN
                     DKVIETLAAAREEAETLDGVHRESLLSQIALGFLRSGSLDLAERTLDLIGDKVLIAST
                     LAGYSMHFDENGERADALETLEEAYQILKSQSEREVRSSKDKFALFGTIALLFAKYEK
                     IERGIEVALEIRDESARYQALNQIAQVCALRDLEDAARETVDLIDEDSERLFAFIGLS
                     DVERKAEKRDEALIYLKEAHNYCGRVPQLSARSQAYNQIARRFHDLGDAATARAAAHE
                     NLETISQIRDESAKAVSLLHLSDVYKSLNFDLTGDERDTLRTMLKKAEW"
     gene            complement(2453..>2973)
                     /locus_tag="VGB68_07700"
     CDS             complement(2453..>2973)
                     /locus_tag="VGB68_07700"
                     /inference="COORDINATES: protein motif:HMM:NF012500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049153.1"
                     /translation="AGVCLTTVRNRKERPVNIAKLQAQDAKSKNDHPAAGARRPALSS
                     DVWLLTDAGFNILLSMETYKWYYQLLSAGRAGETHNFPYKLAGPLCDGGDVYFDIEGE
                     GRLPDYRLLPENVKPDEVLALLNCGAYSVAQMFQYNGRELPAIVLIKKDGQVELIRKR
                     DVYEDLYANDIW"
BASE COUNT          743 a          779 c          797 g          654 t
ORIGIN      
        1 gcggtaagtt gcaattgaaa aatcacgaac ttaccgctta ccgtttaccg cttaaaaaga
       61 ggattttaac tacagtatta tggtaagagc tattttaatg gatttcaacg gcgtgattat
      121 cgacgacgag cctttgcaga tgaaggctta cgccgagctt ttaaaggacg aaggcatcga
      181 gctgaccgaa gaaggttatt attcgtgcgg cgggatggac gacgcgactt tcctgcgcga
      241 gcagtttaaa cgcgcgaaca aagacatcgg cgagacgaaa atcgccgagc tgattcaaaa
      301 gaaaacttca aaatggcgcg agattgtcac cgacaaagtg cctctgttcg aaggggctga
      361 aaattttatc aagaaggcga agcagcgtta cgcgatggcg ctggtcagca tggcaaaccg
      421 cgaggaaatc aatcacattc tcgacacgac gggcttaaaa gattatttcg acgtcgtcgt
      481 ttccgccgaa aacgtcgcgg cgtgcaaacc gaacccggaa tgttacgttt cggcttttcg
      541 gcagctcgac gcgattcgct cggcgcaggg tcatcacccg ctcgaacgcc gcgaatgtct
      601 cgtcatcgaa gacgttccgc agggcatcca atcgggcaaa gccgccggta tgaaaacgct
      661 cggcgttttg aacactttcg acgaagcgac aatgcgcgcg gcgggcgccg acgccatcgc
      721 caaaaacctg aacgactggt ttcccgattc aatccagctg gttttttcca aacactttta
      781 aatgaaagtg ccgagtgccg agttctaagt gccgagtcct gagcgccgag ttttgaatgc
      841 ggatttttta cttgacgcta aacacttaaa actcaagttt taaaactcag aactcggcac
      901 ttggaactca gcactttttc caaatatgag agaacatctt ttcagtatcg aagaagccga
      961 aacgagtctt ttccgcaccg cgacgtattt agccgaaaat gtcggcggcg gcgacgggca
     1021 cgccgaggcg atgaaggaaa tcgtcgcgca ttatatcgag aacggagacg tcgacaccgc
     1081 cgcgctgctt gccgatacgg tcgacgaccc gttttcgcgc gaccgtcttt tattgctggt
     1141 cgcggaaaaa tgcgccgaga ttgacgacga cgaatacgct ttgcagctgg tcgaagccat
     1201 cgaagaatcg ggttttcaat cggtcgcccg cgagcgtctg tcgatgcgca aggcggcaat
     1261 cggcgaattc gacaaagcgt ttgagcttgc cgaaagctta aaacattcgt ccgacgcttt
     1321 cgccgtcatc gccgccgagc aatccgccgc aagccttgaa gcggaaagcg aaaaaactct
     1381 cgcgcgcatc gaatttcctt acgccaaagt caacgcttta ttgaatatcg ccgccggtta
     1441 cgaggcgaaa gaagagcgcg agaaggcgaa agcggcaatc gcccgcgcgg tcgaaaccgc
     1501 ccgcgacatc gagtttaagg aagaaaaaat tcgcgcctta caggacgcgg cgacgcattt
     1561 cgcgcacgtt tccgccaacg acaaggtcat cgaaacgctc gccgccgcgc gcgaagaagc
     1621 cgagactctg gacggcgtac accgcgagag ccttctttcg cagattgcgc tcggtttttt
     1681 gcgctccggc tcgctcgatt tggctgagcg cacgctggat ttaatcggcg acaaagtttt
     1741 aatcgcttcg acgcttgccg gatattcgat gcattttgac gaaaacggcg agcgcgccga
     1801 cgctctggaa acgctcgaag aagcgtatca gattctgaaa tcgcagagcg aaagggaagt
     1861 ccgcagctca aaagataaat tcgctctttt cggcacgatt gctcttttat ttgccaaata
     1921 tgaaaagatc gagcgcggca tcgaagtggc tttggaaata cgcgacgaat cggcgcgtta
     1981 ccaggctttg aaccaaatcg cgcaggtttg cgccttgcgc gatttggaag acgcggcgcg
     2041 cgaaaccgtc gatttgatcg acgaagattc cgagcgcctg tttgctttta tcggcttgag
     2101 cgatgtggag cgcaaagcgg aaaaacgcga cgaagctttg atttatctga aagaagcgca
     2161 taattactgc ggacgcgttc cgcagctttc ggcgcgctcg caggcatata atcaaatcgc
     2221 gcgtcgtttt cacgatttgg gcgatgcggc gacggcgcgc gccgccgcgc acgaaaatct
     2281 ggaaacaatc tcgcaaatcc gcgacgaaag cgccaaagcc gtcagccttc tgcatctgtc
     2341 ggatgtgtat aaatcactga attttgattt gaccggcgac gagcgcgaca cgctgcgaac
     2401 gatgttgaaa aaagccgagt ggtaatcgga aggcgaaagg cacagcgaaa tttcaccaaa
     2461 tatcgttggc atataaatct tcgtaaacgt cgcgctttct gattaattcg acctgcccgt
     2521 cttttttgat taaaactatc gccggaagct cgcgcccgtt gtattgaaac atctgcgcga
     2581 cggaatacgc gccgcaattc aataaagcca gcacttcgtc cggcttgacg ttttccggca
     2641 gcaggcggta atcgggcagt cttccttcgc cttcgatgtc gaaatagacg tcgccgccgt
     2701 cgcacaaagg tccggctagc ttatacggaa aattatgcgt ttctccggct cgcccggcgg
     2761 aaagcagttg ataataccac ttgtaagttt ccatcgaaag cagaatgtta aagcccgcgt
     2821 ccgttaaaag ccagacgtcg ctcgaaagcg ccggtcgccg cgcgccggct gccggatggt
     2881 cgtttttcga ttttgcatcc tgcgcttgaa gcttggcgat atttaccggt ctttcctttc
     2941 gatttctgac cgtcgtcagg cagacgcccg cgt
//
LOCUS       DASWNE010000384         2305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_295830, whole genome shotgun sequence.
ACCESSION   DASWNE010000384 DASWNE010000000
VERSION     DASWNE010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295830"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VGB68_07705"
     CDS             <1..254
                     /locus_tag="VGB68_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049154.1"
                     /translation="DVTDKQNRQTRFFISTKTLRVMALEYTQNAVKYARRFYDYRYAQ
                     GTLVPYRTTLSEGARQIEESTVSTVTYGQKVEEDVFLIG"
     gene            335..919
                     /locus_tag="VGB68_07710"
     CDS             335..919
                     /locus_tag="VGB68_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049155.1"
                     /translation="MNKLKRFSVFVIFALFLCFDAQTFAQENKDVKLAEPSYEVVLQV
                     LVASSAGDNNQLPASLAPVVGKLKTTFPFTNYRLTATSLSRVESGGNIDSRSILQEPN
                     ASISAPSFQNWSLIGVKRVAAAAGGRELVQVGTFSFGTRIPIQTGQTVNYDDTGLKIN
                     RLNLAENTPTVVGTMNASKTNELIVLVLTVRPSN"
     gene            1059..1691
                     /locus_tag="VGB68_07715"
     CDS             1059..1691
                     /locus_tag="VGB68_07715"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0049156.1"
                     /translation="MDGETIQNLYAEVWQTAVSFERLSDHALIEATKQGDETAFAEIV
                     SRYRNPITNYLYRILNDYEEAVDLAQETFVRVYFAADRYHTGYAFSTYVYRIATNLAI
                     SEMRRRKRRKLLSLTGLFQPEDGSETDFDPADENPLPDANLLEDEQKQVIARAIAAMP
                     EKYRLPIVLRDIEGKSYEEVAEILNLGLGTTKSRISRARALLKEKLKHYF"
     gene            1697..>2305
                     /locus_tag="VGB68_07720"
     CDS             1697..>2305
                     /locus_tag="VGB68_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049157.1"
                     /translation="MKKELENLTESDEKVRELLASLKQVEAPKDFEFRLKARIAKASP
                     NAYQTNYKRRFAYALPAFASVLVSTFVVLNGNFFGGGEQVSSIPDTRAQPVNISQQQN
                     PPKEFIASFDTVDKKEPEKITPASLNSGIEKNRSDESPFVAVKAPEKSKNEPRRQLNR
                     ENDSFSKDSALTTPKVFRPRGLNPDTRIEAPKDFNSEKSFSVQ"
BASE COUNT          627 a          558 c          592 g          528 t
ORIGIN      
        1 tggacgtgac cgacaagcaa aatcgccaga cgcgcttttt tatcagcacg aaaaccttgc
       61 gcgtgatggc gctcgaatac acgcagaacg cggtcaaata cgcgcgccgg ttttacgatt
      121 atcgctacgc gcaaggcacg ctcgtgccct atcgcacgac gctttccgag ggcgcccgcc
      181 agattgagga atcgaccgtc tcgaccgtca cttacgggca gaaagtggaa gaagacgttt
      241 ttctaatcgg ttaatttttg aaactttatt tcggcttgcg tgtgaaaggg tttggaagcc
      301 gccgctttag caaaccattt ttacaggaga atatatgaac aagctgaaaa gatttagtgt
      361 ttttgtaatt tttgcattat ttttgtgttt cgacgcgcag actttcgcgc aggaaaacaa
      421 agatgttaaa ctagccgagc cgagttacga agtagttttg caggttctgg tcgcgtcgag
      481 cgcgggcgat aacaaccagc tgccggcgtc gcttgcgccg gtcgtcggaa aattaaagac
      541 tacgtttcct tttacgaatt accgcctgac ggcgacttct ctgagccggg tcgaaagcgg
      601 cggcaatatc gacagccgca gcattttgca ggagccgaac gcctcgattt cagcgccgtc
      661 gtttcaaaac tggtcgctta tcggcgtcaa gagagttgcc gccgcggcgg gcggtcggga
      721 gttggttcaa gtcggcactt tcagtttcgg cacgcgaatt ccgattcaaa ccgggcaaac
      781 cgtcaattac gacgacaccg ggttgaaaat caaccggttg aatttggcgg aaaatacgcc
      841 gaccgtcgtc ggcacgatga atgcgtctaa aaccaatgaa ttaattgttt tagtattaac
      901 cgtccgaccg tccaattaaa caatagtgaa tgaaaacgta aaaaaaattc acaaacgaaa
      961 aacttcagga acttttcaag ccgcgcggcg tgttaaaaaa cgttggcggc ttttatcttt
     1021 accaattctt agattagaaa aggcttttgt ttgttgtgat ggatggagaa actattcaaa
     1081 atctgtatgc ggaggtttgg cagaccgccg tgtcatttga acgcttatcc gaccacgcgc
     1141 tgattgaagc gaccaagcag ggcgacgaga cggcttttgc cgaaatcgtc agccgttatc
     1201 gcaacccgat tacgaattac ctgtaccgga ttttgaacga ttacgaggaa gcggtcgatt
     1261 tggcgcagga gactttcgtt cgcgtttatt tcgccgccga ccgttatcac acgggctacg
     1321 ctttttcgac gtatgtttac cgcatcgcca caaatctggc gatttcggaa atgcgtcgcc
     1381 ggaaacgccg caaattgctt tctttgacag gactttttca gccggaagac ggcagcgaaa
     1441 ccgattttga cccggcggac gaaaaccctc tgccggatgc gaatctgctg gaagacgagc
     1501 agaaacaggt catcgcgcgg gcgattgcgg cgatgccgga aaaatatcgt ctgccgattg
     1561 tgctgcgcga tattgaagga aagtcttacg aggaagtagc ggagatttta aatctcggtc
     1621 tggggacgac caaatcgcgc atctcgcgcg cgagggcttt actaaaagaa aaattgaaac
     1681 attattttta aacgaaatga aaaaagaatt agaaaatttg actgaatcgg acgaaaaagt
     1741 tcgtgagctt ctcgccagcc tcaaacaggt cgaagcgccg aaagatttcg aatttcgcct
     1801 gaaagcgcgc atcgccaagg cgagcccgaa cgcctaccaa acaaattata aaagacgctt
     1861 tgcctacgct ttaccggcgt ttgcctcggt gctggtttcg actttcgtcg tgctgaacgg
     1921 aaactttttc ggcggcggcg agcaagtcag ctccattccc gacacgcgcg cgcagccggt
     1981 caatatcagt caacagcaaa acccgccgaa agaatttatc gcgtccttcg atacggtcga
     2041 caaaaaagag ccggaaaaaa tcactccggc aagcttaaac agcgggattg aaaaaaacag
     2101 gagcgatgaa tcgcctttcg tcgccgttaa ggcgcccgag aaatcgaaaa atgagccgcg
     2161 ccggcaatta aacagggaaa acgacagctt ttcaaaagac agcgccctga cgacgccgaa
     2221 agtctttcga ccgagaggct taaatcccga taccaggatc gaggctccga aagatttcaa
     2281 tagcgaaaaa tctttttcgg tgcag
//
LOCUS       DASWNE010000385         3249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_682560, whole genome shotgun sequence.
ACCESSION   DASWNE010000385 DASWNE010000000
VERSION     DASWNE010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682560"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..559)
                     /gene="rplL"
                     /locus_tag="VGB68_07725"
     CDS             complement(173..559)
                     /gene="rplL"
                     /locus_tag="VGB68_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEY0049158.1"
                     /translation="MAVTKDEVVEFLKGMTLLEASELVKELEEVFGVSAAAAAVAAAP
                     AAAGGGDAAPAEAEKDSFDVILQSAGAAKINVIKVVREITGLGLKEAKDLVDGAPKAV
                     KEGASKDEAEGIRVKLADAGATVEVK"
     gene            complement(820..1533)
                     /gene="rplJ"
                     /locus_tag="VGB68_07730"
     CDS             complement(820..1533)
                     /gene="rplJ"
                     /locus_tag="VGB68_07730"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEY0049159.1"
                     /translation="MKTRAEKQEILNTLTEGFQNSKSAMVVSFNKLTVSKDQEFRNQL
                     RNVGANYQVVKNTLARLAVKGTQLEGATEHFKGVTAVAWTNDDPVVLSKTVSKFVKDN
                     GEVFTFKIGVVDGKVVDLKQVETIASLPSKEELISKILFLLNAPAQRIATVINAVPRN
                     LAVVIKQIGDAKPQTQPEQKTAEPAAESTEESAPVEEVKQEETAAVPEAGAGTQETES
                     AAAEEAPAAAPEAEEKSEE"
     gene            complement(1552..2247)
                     /gene="rplA"
                     /locus_tag="VGB68_07735"
     CDS             complement(1552..2247)
                     /gene="rplA"
                     /locus_tag="VGB68_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEY0049160.1"
                     /translation="MAKQGKKYRAALEKIEPNRKYSLEEAIGKVKEIAFAKFDETVEL
                     TMWLGVDPRKADQMVRGTVVLPNGLGGKAKVVVVIAQGDKIREAEEAGADLAGGEEIV
                     DRIKGGWLEFDALIATPDMMGKVGQLGKILGPRGLMPNPKTGTVTMDVRTAIQETKAG
                     KVEYRVDKTGVIHAPVGKVSFDAEKLQQNTKVLMDAVVKAKPQTAKGRYVKKVNLAAT
                     MSPGVLLDELVII"
     gene            complement(2371..2799)
                     /gene="rplK"
                     /locus_tag="VGB68_07740"
     CDS             complement(2371..2799)
                     /gene="rplK"
                     /locus_tag="VGB68_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEY0049161.1"
                     /translation="MAKKVTGYIKLQIPAGKANPAPPIGPALGQHGVNIMEFCKAFNA
                     KTANDDPDMKVPVVITVYADRSFTFETKTPPAADLLRKASGIAKGSATANKAKVGTIS
                     REKLEEIAKLKMPDLNTTNLESAVKTIEGTAKSMGLTVEG"
     gene            complement(2939..>3249)
                     /locus_tag="VGB68_07745"
     CDS             complement(2939..>3249)
                     /locus_tag="VGB68_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="KOW motif-containing protein"
                     /protein_id="HEY0049162.1"
                     /translation="KSTPKVTGFLGGKNPTPLTQEEVDQIVHNVEIAAEKPKPKFSYA
                     IGEIVRIKSGPFASFTGKVEEVNEEKTTLKVTVTIFGRSTPVELSFLDVEKVTFAEEE
                     "
BASE COUNT          664 a          892 c          703 g          990 t
ORIGIN      
        1 cgaaataaat tttctgagtg tgccgaagaa ggacacctat tctcccgttt tatatcggaa
       61 aaaacaaacc caaaaactca aagcctgctt tgagcttcaa aagagcgaaa tcggcaggcg
      121 atactttttc ctatcggtaa aataccgccc gccttttatt ctaacaagca aactatttaa
      181 cttcaactgt cgcgccggca tctgccagtt tgactctgat gccttcagct tcgtctttgg
      241 atgcaccttc ttttacagct ttcggcgcac cgtcaaccaa atctttggct tctttcagac
      301 ccaagccggt gatttcgcga acgactttga taacgttgat tttcgccgcg cccgccgatt
      361 gcaggataac gtcgaacgaa tctttttccg cttccgccgg agccgcatca ccgccgcccg
      421 ctgctgccgg agcggctgca accgccgccg ccgccgccga aacgccgaaa acttcttcca
      481 actctttcac gagttcgctt gcttccagaa gggtcatacc cttcaaaaat tcaactactt
      541 catcttttgt aactgccatg attatttaat ctctccttat ttagggttct tttgtttatt
      601 gttttaaaac tatctctagc ggatagtttc gctttatgtc agccgtcaga acccacggga
      661 taatttcttc aaggttgcat ctccatccga atttatccga gccgcaaagg cgacctgacg
      721 cgtcgcttcg ctcgttttcc ttggctacgg cttttgaaat tcgcgatttt caaattgaaa
      781 tattcttatt caatcgaaaa tctaaaatct aaaatttctt tactcttccg atttttcttc
      841 tgcttccggc gcggcggctg gagcttcctc agcggcggcg ctttcagtct cctgcgttcc
      901 ggcgcctgct tccggaaccg ctgccgtttc ttcctgtttc acttcttcaa caggcgcaga
      961 ttcttcagtg ctttccgctg ccggttcagc cgttttttgt tcgggctgag tctgcggctt
     1021 cgcgtcgccg atttgcttaa tgaccacggc gagattgcgc ggaacggcat tgataaccgt
     1081 cgcgatgcgc tgcgccggag cattgagcag aaacagaatc ttgctgataa gctcttcttt
     1141 cgacggcagc gaggcgattg tttcgacttg tttcaagtcg acgacctttc cgtcgacgac
     1201 accgattttg aaggtaaaca cttcgccgtt gtccttgacg aattttgaaa ccgttttgga
     1261 taaaaccacc gggtcgtcat ttgtccaggc gaccgccgtc acgcctttaa aatgttcggt
     1321 tgcgccttcc agctgcgtgc ctttgacggc taatctcgcc agagtatttt tgaccacctg
     1381 ataatttgcg ccgacattgc gcagctgatt gcgaaattcc tggtctttgg agacggtcaa
     1441 tttattaaaa ctgacgacca tcgccgactt tgaattctga aacccttcgg tgagggtatt
     1501 taaaatttcc tgtttttctg ctcttgtttt cattttttat tccctcactc cttagattat
     1561 aactaattcg tcgagcagaa cgcccgggct catcgtcgcc gccagattca ctttcttaac
     1621 gtagcgacct ttcgccgtct gcggtttcgc cttgacgacg gcatccatca aaactttcgt
     1681 attctgctga agcttttcgg catcgaaaga aacttttccg accggagcgt gtatgacgcc
     1741 cgttttatcg acgcgatatt ctaccttacc ggctttcgtt tcctgaatcg ccgtgcgcac
     1801 gtccatcgtc accgttccgg ttttcggatt cggcatcagc ccgcgcggtc ccaggatttt
     1861 tccgagctga ccgactttgc ccatcatatc gggcgtcgca atcagcgcgt cgaattccag
     1921 ccagccgcct ttgatgcggt cgacgatttc ctcgccgccc gctaaatccg ctccggcttc
     1981 ttcggcttcg cggattttat cgccctgcgc gataacgacc acgactttcg ccttgccgcc
     2041 cagaccgttc ggcagaacga ccgttccgcg caccatctgg tcggctttgc gcgggtcgac
     2101 gccgagccac attgtcaatt cgacggtctc gtcaaatttg gcaaaagcaa tttccttgac
     2161 tttgccgatc gcttcttcga gcgagtattt tctgttcggc tcgatttttt cgagagccgc
     2221 tctgtatttt ttaccttgtt tagccatttt acctccaggt tttaacgaag tattttaact
     2281 tctccctttt tctaacggcg aaggacgaag ggcgaaagga taaagtaaaa aaatgtcttt
     2341 tcctaatctt tcgcctttat ccctcagcct ttacccttca accgttaagc ccatgctttt
     2401 cgccgtgcct tcgatggtct taaccgccga ttccagattc gtcgtgttca aatccggcat
     2461 tttcaattta gcgatttctt cgagcttttc gcgcgatatc gttcccactt tagctttatt
     2521 agccgtggcg gagcctttgg cgatgcccga tgctttgcga agcaaatcag ccgccggcgg
     2581 cgttttcgtt tcaaacgtaa acgagcggtc ggcgtaaacc gtaatcacga ccggcacttt
     2641 catgtcggga tcgtcgttcg ccgttttcgc gttgaacgct ttgcaaaact ccatgatgtt
     2701 aacgccgtgt tgtccgagtg cgggaccgat cggcggcgcc ggattcgctt ttccggcggg
     2761 aatctgtagt ttgatataac ctgtaacttt tttcgccata atgcttgatt tacgatttcg
     2821 gatttttatt aaagacgacc cgaaaatgtc ctcattcttt gtaactgttt ataaaaattt
     2881 gcggttcgca gattttcaat aaatcgctga acacgcaaat ctaaaatcta aaattccttt
     2941 attcttcttc ggcaaacgta actttttcga cgtccagaaa actcaattca accggcgtcg
     3001 agcgcccgaa aatcgtcacc gttactttca aagtcgtttt ttcttcgttt acttcttcga
     3061 ctttgccggt aaagctggca aacggaccgg acttgattct cacgatttcg ccgatggcgt
     3121 aggagaattt cggtttcggt ttttccgctg cgatttcgac gttgtgaaca atctggtcga
     3181 cttcttcctg cgtcagcggc gtcggatttt ttccgcctaa gaaaccggtg actttcggcg
     3241 tcgatttta
//
LOCUS       DASWNE010000386         7766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_260587, whole genome shotgun sequence.
ACCESSION   DASWNE010000386 DASWNE010000000
VERSION     DASWNE010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7766
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260587"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VGB68_07750"
     CDS             complement(<1..546)
                     /locus_tag="VGB68_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049163.1"
                     /translation="MKVLKFGGSSVGNAENIERVFEIVKNALEKDSCAVVLSAMQGTT
                     DALIEVGKAAEQGSEIFRDKITAIKEKHRAAVGALMPENSRKEISDFVDSSIAELEKI
                     CEGVFLLRELTARTLDRIVSFGEILSTAIVSAVFDWRQIENVRKDSRELIRTDSNFGF
                     AAVDFSKTDGQIREFFHNSDIK"
     gene            922..1098
                     /locus_tag="VGB68_07755"
     CDS             922..1098
                     /locus_tag="VGB68_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049164.1"
                     /translation="MHKNNENIYQGTEMALALVVLLLNFLILAAVVGIVWSMQLLIAP
                     GASLSIAVGETLWR"
     gene            1218..1730
                     /locus_tag="VGB68_07760"
     CDS             1218..1730
                     /locus_tag="VGB68_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049165.1"
                     /translation="MKIRKNLARLFLFVATVYWGIWLGGYIFNALMMVPLWSHNAPDS
                     IVSHFKTPHFTLYFFSLVNPWVFLTSLIAWLLTIRLDTGARVWLGRATLIAWIMLPLK
                     IWMVITIGGVVGATMDGNFKPEMINTISLWKSLNWLTITTAAVILVFHLLAILNFNNP
                     RATRIETALR"
     gene            complement(1799..3094)
                     /gene="thrC"
                     /locus_tag="VGB68_07765"
     CDS             complement(1799..3094)
                     /gene="thrC"
                     /locus_tag="VGB68_07765"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEY0049166.1"
                     /translation="MQYFSTNRKSAHASFREAVLNGQPPDRGLYFPEAIPALPSDFWK
                     DFRAKTKEQIAFEVIKPFAGAEIPDETLFRICAETVNFDFPLVKITDSIYTLELFHGA
                     TLAFKDVGARFMSRCLRYFSLGKREKTIVIVATSGDTGGAVANGFFDVEGIEVVILYP
                     RGKVSQVQELQLTTPGKNITALEVEGNFDDCQTLAKTALADEELKRLVFLTSANSINI
                     ARWLPQQFYYFYALQQWQAEDAPVIAVPSGNFGNLAAGILAHVAGLPARKFIAACNAN
                     DAVPRFLQSGSFQSKPSVSTLSNAMDVGNPSNFVRILEIFDNDYLNLKEKMEARSVSD
                     ESTAAAMREVYQKYGYVLDPHGAVGFDALSEYLRKNPNQKGFFLETAHPVKFDSVKKI
                     VGTHGETPEAVRDLLNREKRSVEIKANYEDLKEILLGKI"
     gene            3251..4171
                     /locus_tag="VGB68_07770"
     CDS             3251..4171
                     /locus_tag="VGB68_07770"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEY0049167.1"
                     /translation="MKEISVLAPASVSNVVCGFDCLGFALEAPCDEMTVRLIDEKTVK
                     IINHDRYNLPTAAEKNVAGAALLAMLDEIEDRIGFEVEITKRIKPGSGIGSSAASAVG
                     AVAAANRLLENRFSRLQLAEFAMSGEFVASQARHADNVAPCLFGGFVLVRSAAPLDIV
                     SLEFPALHATVIHPQIEIKTSEAREILPENVPLKDAIRQWSNLGALVAALSKGDYGLI
                     SRSLEDFLIEPVRKALIPRFDDLKSASLKAGALGGGISGSGPSVFMLSETLETARKVE
                     TAMREIYERTQIDFYIYVTKINEEGVKFVR"
     gene            4177..4476
                     /locus_tag="VGB68_07775"
     CDS             4177..4476
                     /locus_tag="VGB68_07775"
                     /inference="COORDINATES: protein motif:HMM:NF022359.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2695 domain-containing protein"
                     /protein_id="HEY0049168.1"
                     /translation="MTTISEKIRRNQIKRTIKNSEREEILKTLPVQPGVLRKCFDFLN
                     ERLSKNGCDDTLNFTLEFIQKNNLPQGDLTYWLENNGGFCDCEVLANVEEKINDE"
     gene            4469..5467
                     /locus_tag="VGB68_07780"
     CDS             4469..5467
                     /locus_tag="VGB68_07780"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF003384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0043857 - N-acetylornithine
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylornithine carbamoyltransferase"
                     /protein_id="HEY0049169.1"
                     /translation="MNNFLKTSDFSRAELEEILESALRFKKGETGEKPLSGKSVALVF
                     FNPSLRTRASMQVGVYELGGNAVILEPGSSSWTLEHRDGVVMDGDKTEHLKEFVRVLE
                     RYVSAIGVRTFAELKDWEAERAEPVLSAFAKYATKPVINLESAMHHPCQSMADMLTIR
                     ERFGKAKRRVLLTWAWHPKPLPMAVPNSFALAAAQFGHDLRIAHPKGFELDAELMSEI
                     ENQARENGGSVEITNDIYSAFDEAEVVYAKSWGSFRHYGNLQKDIGERAVYRGKWIVD
                     EAKMRRTRDAIFMHCLPVRRNVIVTDGVIDAPNSLVIDEAENRLHVQKAIMAKLIK"
     gene            5464..6357
                     /gene="argB"
                     /locus_tag="VGB68_07785"
     CDS             5464..6357
                     /gene="argB"
                     /locus_tag="VGB68_07785"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEY0049170.1"
                     /translation="MNDENLNLLREALPYIQRFKGKTFIVKLSGKVTEERENLLSLTE
                     ELALLHQVGIRLCVVHGGGKQLSQLAAKLGVVQTVIEGRRVTDDDTLELAKMIFAGKI
                     NTDILAALRNRGIEAVGLSGIDGDVVKAVRRPPKEITNKQTGATETIDFGHVGDVVEI
                     NSRLLHLLLENDYLPVISSLGADDEGRVFNINADTIASEIAVQLNAEKLVLLSDVNGI
                     YTDAANPETKLSRLSAAEAEAMIADGRASGGMIPKLQNLISLLRRGVKSAHIIDGTRR
                     NTLLSEVFTDEGTGTMVVTDS"
     gene            complement(6423..7475)
                     /locus_tag="VGB68_07790"
     CDS             complement(6423..7475)
                     /locus_tag="VGB68_07790"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0049171.1"
                     /translation="MLSVAAAAQKIGKPTLVPTEATEAQTQLIQQGIRLHDQKKYDEA
                     IKKYEQVLQQNPNNDFALYEMALSLYNKKDFPKALDASYKLVQYKGKTGLLGYGLIAN
                     ILDDEGKPKEAIEIYRKAIKQLDGDPEYQAQVSSLYFNLGVTYVRQKQYKESREALKK
                     AVQLDFSYPSPHYLLAEVYQGSKYKVPALLAAARFITMEINSQRAQRSVTIFLDTLKS
                     AKKDEKTGNINIFLDLNAPKDEGDFGMYELLLGTLTTVKDEKDKNKTEEEIFADAVDT
                     LIGLLEGDKKLASTFVGKTYVPFMVDMKKNGYSKIFAYLVLQQQGNKQAEKWLIDQGQ
                     KTIDFINWAKTYQLRK"
BASE COUNT         1898 a         1913 c         1963 g         1992 t
ORIGIN      
        1 tttaatatct gaattatgaa aaaattccct gatttgcccg tcggtttttg aaaaatcgac
       61 cgccgcgaaa ccaaaattcg aatcggtgcg aatcagctcg cgcgaatctt tccggacgtt
      121 ttcgatttgc cgccaatcaa atacggcgga aacgattgcg gtcgaaagta tctcgccgaa
      181 gctgacgatt ctatccagcg tccgcgccgt cagctcgcgc agcagaaaca cgccttcaca
      241 gattttttcg agctccgcga tgctcgaatc gacaaaatcc gaaatctctt ttcgactgtt
      301 ttccggcatc agagcgccga ccgccgcgcg gtgtttttct ttgattgccg tgattttgtc
      361 gcgaaaaatt tcagagcctt gttcggcggc ttttccaacc tcaatcaaag cgtcggtcgt
      421 gccctgcatc gccgacaaaa cgacggcgca cgaatctttt tcgagcgcgt ttttaacgat
      481 ttcgaaaact ctctcgatat tttcggcgtt tccgacggac gagccgccga attttaaaac
      541 tttcatattt caggctttgt cttaaaagcc cgcttttccg aaggctttta aaacaagttt
      601 aagcgcatcg ggcacaaacc ggaaatcccg acgaaatccg agcctgattt tcagcgcgca
      661 catcgattga atcgacgcgc aaagccactc ggagcgaatc gattccggcg ttcgctccgg
      721 tcgccttatc gtttttgccg ctccgtcgtt gaagaattcc gtataatttg tcgccgcttt
      781 tcttccgttt caaaaatttg tcaaaggata ttttctttcc ggtttttagt caaacaattt
      841 tttttatgag cgtttttgca aaagattgtc gcgttaataa ttacagacgg ctcaaatcaa
      901 tttgttttgg aggaaagtga aatgcataaa aacaatgaaa atatttatca gggaaccgaa
      961 atggcgttgg cgctggtcgt attactgctc aattttttaa ttcttgccgc cgtggtcgga
     1021 atcgtctggt cgatgcaatt gttgattgcg ccgggcgctt cgttgagcat cgccgtcggc
     1081 gaaacgctct ggcgataaac tttgaataaa attacaagat aaactttgaa taaaattaca
     1141 agaaaaactt tgaataaaat tataagagcg cgcggcgcgt cattcaatga cgacgccgac
     1201 cgcaggaggc aaaaagtatg aaaattcgta aaaacctggc tcgcctgttt ctattcgtcg
     1261 cgaccgttta ttggggaatc tggctcggcg gctatatctt caacgccctg atgatggttc
     1321 ccttgtggag tcataatgct cccgattcga tcgtcagcca ctttaaaacg ccgcatttta
     1381 cgctttattt tttttcgtta gtcaatccgt gggtctttct gacatctctg attgcctggc
     1441 tcctgaccat caggctcgac accggagccc gggtctggct gggcagggcg acgctcatcg
     1501 cgtggattat gctgccgctg aagatttgga tggttataac catcggagga gtggtcgggg
     1561 cgacaatgga cggcaatttc aagccggaga tgataaatac gattagctta tggaaaagct
     1621 tgaactggtt gaccattacc accgccgccg taattcttgt ttttcattta ctggcaatcc
     1681 tgaatttcaa taatccgaga gcgacacgaa tcgagacggc attacggtaa ggcggcaaaa
     1741 gagctgtctg agggagcaga ttgtttgaag ccgctctgaa tctcgtcatt ctctgcggct
     1801 aaatctttcc cagcaaaatt tctttcaaat cctcgtaatt tgcttttatc tcgacgcttc
     1861 gcttttcgcg attcagcaaa tctcgcacgg cttcaggtgt ttcgccgtga gttccgacaa
     1921 tttttttcac cgaatcgaat ttaaccggat gcgccgtttc gagaaaaaag cctttctgat
     1981 tcggattttt ccgcaaatat tccgacaaag cgtcaaagcc gaccgcgccg tgcgggtcga
     2041 gcacgtagcc gtatttttgg taaacttcgc gcatcgcggc tgccgtcgat tcatccgaga
     2101 cgctgcgcgc ctccattttt tctttcagat ttaaataatc gttatcgaaa atttccagaa
     2161 tgcgcacgaa attcgaaggg ttgccgacgt ccatcgcgtt ggaaagcgtc gagaccgaag
     2221 gcttcgattg aaaactcccg ctttgcaaaa accgcggaac ggcgtcgttg gcattacaag
     2281 ccgcgataaa ttttcgggct ggcaatccgg cgacgtgagc cagaattccc gccgcgagat
     2341 tgccgaaatt tccgctcgga acggcgataa cgggcgcgtc ttccgcctgc cattgctgca
     2401 gagcgtaaaa ataataaaac tgctgcggca gccagcgcgc aatatttatc gagtttgccg
     2461 acgtcagaaa aaccagtctt ttcaattctt cgtcggcgag agccgttttc gccagagtct
     2521 ggcaatcgtc aaaatttccc tcgacttcca gtgccgtgat gtttttgccg ggcgtcgtca
     2581 attgcagctc ctgcacctgg ctgactttcc cgcgcgggta caaaatcacg acttcaatgc
     2641 cttcgacatc gaaaaaaccg ttggcgaccg cgccgcccgt gtcgccggaa gtcgccacga
     2701 tgacgattgt tttttctcgt ttgcccagcg aaaaataccg caggcagcgg ctcataaaac
     2761 gcgcgccgac gtctttaaaa gcgagcgtcg cgccgtgaaa aagctcgagc gtgtaaatcg
     2821 aatccgtgat tttaacgagc ggaaaatcga aattgacggt ttccgcgcaa atccgaaaaa
     2881 gcgtctcgtc cggaatttcc gcgccggcaa acggttttat gacttcaaaa gcgatttgct
     2941 cttttgtttt cgcccggaaa tctttccaaa aatcagacgg aagcgccgga atcgcttccg
     3001 gaaaatataa gcctctgtcc ggcggctgac cgtttaaaac ggcttcgcgg aaagacgcgt
     3061 gagccgattt tcggtttgtg ctaaagtatt gcattgtctg ggaaaacagg aacgaaaccg
     3121 cagatttgcg caggttaacg cggactattt tttattttat ccgggtgttt ttgcgtttgg
     3181 ctgcgatttc atattttatt tataaattca aaggtcttat gataatacga atttcgcctt
     3241 ttgctaaatt atgaaagaaa tcagcgtact tgcgcccgcc agcgtttcaa acgtcgtctg
     3301 cggatttgat tgtctcggtt ttgcgctcga agcgccgtgc gatgaaatga ccgttcgcct
     3361 gatcgatgaa aaaacggtga aaattatcaa tcacgaccgg tataatctgc cgaccgccgc
     3421 cgaaaaaaac gtcgccggag cagcgcttct ggcgatgctc gacgaaatcg aagaccgaat
     3481 cggctttgaa gtcgaaatca ccaagcgcat caagcccgga agcggcatcg gttcgagcgc
     3541 ggcaagcgcg gtcggcgcgg tcgcggcggc aaatcgcctg ctggaaaatc gtttttcgcg
     3601 ccttcagctc gccgaatttg cgatgtccgg cgaattcgtc gcttcgcagg cgcgccacgc
     3661 cgacaacgtc gcgccgtgtc ttttcggcgg tttcgtcctg gttcgctccg ccgctccgct
     3721 cgatatcgtc tcgctcgaat ttccggcgct gcacgcgacg gtcatccacc cgcaaatcga
     3781 aatcaaaacg tctgaggcgc gcgagattct gcccgaaaat gttccgctca aagacgcgat
     3841 tcgccagtgg agcaatctcg gcgcgctggt cgccgcgctg tcgaaaggcg attacggact
     3901 gatttcacgc tcgctcgaag actttttgat tgagccggtg cgcaaggcgt tgattccgcg
     3961 ttttgacgat ttgaaaagcg cgagcctgaa agcgggcgcg ctcggcggcg gaatttccgg
     4021 ctcgggtccg agcgttttta tgctgagcga aactttggaa acggcgcgaa aagtcgaaac
     4081 cgcgatgcgc gaaatttacg agcggacgca aattgatttt tatatttatg ttacgaaaat
     4141 taatgaagaa ggcgtgaaat ttgtgaggtg aatcttatga cgacaatatc tgaaaagata
     4201 agacgaaatc aaatcaaaag aaccataaaa aattcggaaa gagaagagat tcttaaaacg
     4261 ctcccggtac aacctggagt gcttcgaaag tgcttcgact ttctcaacga gcgtttatct
     4321 aaaaatggtt gcgatgatac attaaatttt actttggagt ttattcagaa aaataacctg
     4381 ccgcaaggtg atttaactta ctggctggaa aataacggcg gcttttgcga ctgcgaagtt
     4441 ttggcgaatg tagaagagaa aatcaacgat gaataatttc ctaaaaacat cagatttttc
     4501 ccgcgccgag ctggaagaaa ttctggaatc ggcgctgcgt ttcaaaaagg gcgagaccgg
     4561 cgaaaaaccc ctgagcggaa aatccgtcgc gctcgttttt ttcaatccca gtttgcggac
     4621 gcgcgcgtcg atgcaggtcg gcgtttacga gctcggcgga aacgccgtga ttttagaacc
     4681 cggaagctcg tcgtggacgc tcgaacatcg cgacggcgtc gtgatggacg gcgataaaac
     4741 cgagcatttg aaagaatttg tgcgcgtgct ggaaagatac gtctcggcaa tcggcgtgcg
     4801 gacttttgcg gaattgaaag attgggaagc ggaacgcgcc gagccggttt tgtcggcgtt
     4861 tgccaaatat gcgacgaagc ccgtgattaa cctcgaatcg gcgatgcatc acccgtgtca
     4921 gtcgatggcg gatatgttga ctattcgcga aagattcggc aaggcgaaaa gacgcgtttt
     4981 gctgacctgg gcgtggcatc cgaaaccgct gccgatggct gttccgaata gtttcgcgct
     5041 cgccgccgcg cagttcgggc acgatttgcg catcgcgcat ccgaaaggct tcgagcttga
     5101 cgcggaattg atgagcgaaa tcgaaaatca ggcgcgggaa aacggcggca gcgtcgaaat
     5161 aacgaacgat atatattcgg cttttgacga agcggaagta gtctacgcga aaagctgggg
     5221 cagttttcgg cattacggca atttgcagaa agacatcggc gagcgcgccg tttatcgcgg
     5281 gaaatggatt gtcgacgaag cgaaaatgcg gcgaacgcgg gacgcgattt ttatgcactg
     5341 tcttcccgtc aggcgcaatg taatcgtcac ggacggcgtg attgacgcgc cgaattcgct
     5401 ggttatcgac gaagccgaaa accgcctgca cgttcaaaaa gcgattatgg caaaattgat
     5461 aaaatgaatg atgaaaactt aaatcttctg cgcgaggcgc tgccttatat tcaaagattt
     5521 aaaggcaaga cttttatcgt caagctgtcg ggcaaagtca ccgaagagcg tgaaaatctt
     5581 ttgtcgttga ccgaggaact ggcgctgctg catcaggtcg gaatcaggct ttgcgtcgtt
     5641 cacggcggcg ggaaacagtt gtcgcagctt gccgccaagc tcggcgtcgt gcaaaccgtt
     5701 atcgaaggtc ggcgcgtgac ggacgacgac acgctggagc tggcgaaaat gattttcgcg
     5761 ggcaaaatca acacggatat tctcgccgcc ctgcgcaatc gaggcatcga agccgtcggt
     5821 ctttccggca tcgacggcga cgtcgtgaaa gccgtccgcc gcccgccgaa agaaattacg
     5881 aacaaacaaa cgggcgcgac cgaaacaatc gatttcggtc acgtcggcga tgtcgtcgag
     5941 ataaattccc gccttttgca cctgcttctg gaaaacgatt atctgcccgt gatttcctcg
     6001 ctcggcgccg acgacgaagg cagagttttt aatatcaacg ccgatacgat tgcttcggaa
     6061 atcgccgttc agctaaacgc cgaaaaactg gttttgctgt cggacgtcaa cggcatctac
     6121 acggacgccg ccaatcccga aacaaagctt tcgcgccttt cggcagcgga agccgaagcg
     6181 atgattgccg acgggcgcgc gtccggcggg atgattccga agctgcaaaa cctgatttcc
     6241 ctgctccggc gcggcgtcaa atcggcgcac atcatcgacg gcacgaggcg caatacgctt
     6301 ttgtccgagg tttttacgga cgaaggaacc gggacgatgg tggtaactga tagttgataa
     6361 ctgatagttg atagtaaaag caaaacaact atcagttatc agttatcaac tatcagttaa
     6421 ttttattttc ttaattgata agttttcgcc caattgatga aatcaatggt tttttgtccc
     6481 tggtcaatca gccatttctc ggcttgcttg ttgccctgct gctgcaaaac cagataggcg
     6541 aagattttgg aataaccgtt tttcttcata tcgaccataa acggaacgta agtttttccg
     6601 acaaacgtcg aggcgagctt tttatctccc tctaaaagcc cgatgagcgt atcgaccgca
     6661 tcggcgaaaa tttcctcttc ggtcttgttt ttatcttttt cgtccttgac ggtcgtcaga
     6721 gtgcccagaa gcaattcgta cataccgaaa tcgccttcgt ccttcggcgc gttaaggtcg
     6781 agaaaaatgt tgatgttgcc ggttttttca tcttttttcg cgctcttgag agtgtcgaga
     6841 aaaatcgtga ccgaacgctg cgcgcgctgc gagtttatct ccatcgtgat aaaacgcgcc
     6901 gccgccagca gcgccggaac cttgtatttc gagccctgat aaacttccgc cagcagataa
     6961 tgcgggctcg ggtagctgaa atcgagctgg acggcttttt tcagagcttc gcgcgattcc
     7021 ttatactgct tctgccgcac gtatgtgacg ccgagattaa agtaaagact cgatacctga
     7081 gcttgatatt ccgggtcgcc gtcgagctgt ttgatggctt tccggtaaat ctcgatcgcc
     7141 tctttcggct tgccctcgtc gtccagaata ttggcaatca agccgtaacc gagcaagccg
     7201 gtttttcctt tatactgaac gagtttataa gacgcatcga gcgctttcgg aaaatctttt
     7261 ttgttgtaaa gcgaaagcgc catctcgtag agagcaaagt cgttgttcgg attttgctgc
     7321 aagacctgct cgtatttttt aatggcttcg tcatattttt tctggtcgtg aagcctgatg
     7381 ccctgttgaa taagctgcgt ttgcgcctcg gtcgcctcgg tcggaaccag cgtcggcttg
     7441 ccgatttttt gcgccgcggc ggcaactgaa agcacaagac aaaataatat tgtcaaataa
     7501 atattttttc tcattaaatc ctcgattttt attttattga aaaactgccg gctatttgat
     7561 gcgttggctg gttatttgat gcgttaaacg gaaaaaagtt ccagtaaaaa aattatttac
     7621 tttccgcagc cgggaaaagc ctgtcgattt ccgcttttgt ccaatcgcag agaatccgaa
     7681 tttcttcgtc cgacattacg gcgtccgtgt gaatccacag atacgacggc agaggcatcc
     7741 ggcgcgtctc gacctgctcg cagact
//
LOCUS       DASWNE010000387         3285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_47899, whole genome shotgun sequence.
ACCESSION   DASWNE010000387 DASWNE010000000
VERSION     DASWNE010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47899"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VGB68_07795"
     CDS             complement(<1..680)
                     /locus_tag="VGB68_07795"
                     /inference="COORDINATES: protein motif:HMM:NF017508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEY0049172.1"
                     /translation="MKTEVIEVSPTQKEIKIEIDGDTVRDVYNKIAKKYAQAVTVPGF
                     RKGFAPVDIVKMRYKDEIKNETLRELLPDRVSEAIQENNLNPLGEPQLHLENAENIKL
                     NGSEPISLHVHVEVMPEIPTPDYRKLEGVRRVRPVDDEELERVIDQRRQQSASLIPVE
                     DRKSQEGDTVIADLEGTFVGDAENPEPIKVEDLEIKLGDAGIEKTFSENLTGVEEDEE
                     KEFTVEYPA"
     gene            complement(837..921)
                     /locus_tag="VGB68_07800"
     tRNA            complement(837..921)
                     /locus_tag="VGB68_07800"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(885..887),aa:Leu,seq:tag)
     gene            complement(1129..>3285)
                     /locus_tag="VGB68_07805"
     CDS             complement(1129..>3285)
                     /locus_tag="VGB68_07805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEY0049173.1"
                     /translation="RAFFEHYGVNFRGILRALWRRYEGDDNSPIARQGGSSITQQLVK
                     NLFLTPEKSYTRKAEEAYMSVILETRLTKEEIFTLYANQIYLGQQSGASIYGVGEAAN
                     AYFGKDVSQLNLAEAAFLAGIIRSPNRYNAYKNPERVAERRNQVLQSMLDAGEISPQQ
                     FDEIKKTPINLVQVSSRPELLDMPYFTQFAEEELPKIVNDPEALQHLRVYTTIDVDLQ
                     RAAYEAVTKRLERLEKHFPKKEKGNLQASLVAIKPKTGEIVAMIGGRDFQQNQFNRAT
                     SAQRQPGSVFKPFVYAAALSSAYDPASRVYTAATMFKDEKKTFTFNQDTYSPNNYGDT
                     FSNKELTLRDALTKSKNVITVDLAMELNVGKVMNLAHKAGLPKVERAYPSMALGTAEA
                     TPLQMATAYTIFANLGDKVSPLAINRVTTGDGRTVVAPVAERKNVLRPDVAYIMNDIM
                     KDVINKGTAAEAQAWGFKNIAGKQGIAGKTGTSRDGWFAGFTPNLVCIVYVGFDDGSD
                     LGMKGSDSAMPIWADFMRTALDLHPEWNGDWIQPDSVRKAEIDVRNGKLIRELDNTQA
                     ESIQAQQNVLKKNANANANTANPLPTPAGPNDLFVSNVPPEFRRIELFVSGTVPLKLL
                     PTEDAEPDLESEANPEPTATPFQTWTEEQNGKTKTENLPGNSNRQPNYERSLMVTVCP
                     LSGMRATANCPIKETQNFKAGAEPKDFCTFHTKPPE"
BASE COUNT          690 a          874 c          826 g          895 t
ORIGIN      
        1 gccggatatt cgaccgtaaa ttctttttcc tcgtcttctt cgacgccggt cagattttcc
       61 gaaaaagttt tttctattcc ggcgtcgccg agcttgattt ccaaatcttc gacctttatc
      121 ggttccggat tttccgcgtc gccgacaaaa gtgccttcca aatcggcgat aaccgtgtcg
      181 ccttcctgcg atttgcggtc ttcgaccgga atcagcgacg ccgattgctg gcgacgctgg
      241 tcgataacgc gttcgagttc ttcatcatcg accgggcgga cgcggcgcac gccttccagc
      301 ttccggtaat ccggcgtcgg aatttccggc atcacttcga cgtggacgtg cagtgaaatc
      361 ggctcggagc cgttcagctt gatattctcc gcgttttcca gatgaagctg cggctcgccg
      421 agcggattca gattgttttc ctgaatcgcc tcggatacgc ggtcgggcaa aagctcgcgc
      481 aaggtctcgt ttttaatttc gtccttgtag cgcattttca cgatatcgac cggcgcgaag
      541 cctttgcgaa agcccggaac ggtcacggct tgcgcgtatt ttttggcgat tttgttgtaa
      601 acgtcgcgga cggtatcgcc gtcaatctcg attttaattt ctttctgtgt cggggaaact
      661 tcgataactt ctgttttcat tcgttaaata aacaacccgt ccgcctcatt taatacggac
      721 gggcaacctc tctttctaaa ttacgaatta caaattacga attacgaatg attgatttat
      781 tttaaacgtc cgaattaatt ccgcccgatt cgtaattcgt aattcgtaat tgattttggt
      841 gccgagggcg agactcgaac tcgcacacct ttcggtacag gctcctaaga cctgcgcggc
      901 tgccaatttc gccacctcgg caaattttca atcacaaact gcaaatttga atttatcgaa
      961 ttagacggta aaaggcaaaa gcgtgaaaaa gtaaaaagca aaaaagcaaa aggcaaaagt
     1021 taaatccgat tgcccggcaa actcgatttt aagaattttg ccgaattcaa attatacttt
     1081 tgccttttta cttttgtctt tttacttttg tctttttact ttttactttt attccggcgg
     1141 tttcgtgtga aacgtacaga aatctttcgg ctcggctccg gctttgaaat tttgcgtttc
     1201 cttaatcggg caattcgcgg tcgcgcgcat tcctgacaaa gggcaaaccg tcaccatcag
     1261 gcttcgctcg taattcggct ggcggttcga attgccgggc aaattctctg ttttcgtttt
     1321 tccgttctgt tcttccgtcc aggtctgaaa cggcgtggcg gtcggctccg gattggcttc
     1381 gctttccaaa tccggctcgg cgtcttcagt cggcaaaagc ttgagcggga cggttccgct
     1441 gacaaaaagc tcgatgcggc gaaactccgg cggaacgttt gatacgaata aatcattagg
     1501 accggcgggc gtcggcagcg gattcgcagt gtttgcattg gcgttcgcgt ttttctttaa
     1561 aacgttctgc tgcgcctgaa tcgattcggc ttgcgtgttg tcgagctcgc ggattaattt
     1621 tccgttgcga acgtcgattt cggctttgcg aaccgaatcc ggctgaatcc agtcgccgtt
     1681 ccattcggga tgcaaatcca gcgccgtccg cataaaatcc gcccaaatcg gcatcgccga
     1741 atccgagcct ttcattccca aatccgagcc gtcgtcaaac ccgacataaa cgatacagac
     1801 cagattcggc gtaaatccgg cgaaccagcc gtcgcgcgaa gttccggttt ttccggcaat
     1861 tccctgtttt cccgcaatat ttttgaagcc ccaagcctgc gcttccgccg ccgtgccttt
     1921 gttgataacg tccttcataa tgtcattcat aatgtatgcg acatcgggac gcaggacgtt
     1981 ttttctttcg gcgacgggcg caacgacggt tctgccgtcg ccggtcgtca cgcggtttat
     2041 cgctaaaggc gaaactttat cgccgagatt ggcaaaaatc gtgtaagccg tcgccatctg
     2101 caaaggcgtc gcctccgccg ttcccagagc catcgacgga tacgcgcgct cgactttcgg
     2161 aagcccggct ttgtgcgcca gattcatcac ttttccgacg ttcagctcca tcgccaaatc
     2221 gaccgtgatg acgtttttcg atttcgtcag cgcgtcgcgc aaggtcaatt ccttgttgct
     2281 gaacgtgtcg ccgtaattgt tcggcgaata agtgtcctga ttaaaggtaa aagttttttt
     2341 ctcgtccttg aacatcgtcg cggcggtgta aacgcgcgac gccgggtcgt aagccgagct
     2401 caaagcggcg gcgtagacga acggcttgaa aaccgagccg ggctggcgct gcgcgctggt
     2461 cgcgcggtta aattgattct gctgaaaatc gcgcccgccg atcatcgcca cgatttcgcc
     2521 cgttttcggc ttgatggcga cgagcgaggc ttggagattt cccttttcct ttttcggaaa
     2581 atgtttttcg agccgttcca gacgctttgt gacggcttcg taagcggcgc gctggagatc
     2641 gacgtcaatc gtcgtgtaga cccgaagatg ctgcaaggct tccgggtcgt tgacgatttt
     2701 cggcagctct tcttcggcaa actgcgtgaa atacggcata tcgagcagtt ccggacgact
     2761 cgaaacctgc accagattaa tcggagtttt ctttatttcg tcgaattgct ggggcgaaat
     2821 ctcgccggcg tcgagcatcg actgcaaaac ctgattgcgc cgttcagcca cgcgttccgg
     2881 atttttatag gcgttgtagc ggttcgggct gcggataatt ccggcgagaa aagccgcttc
     2941 cgccagattt aattgcgaaa cgtctttgcc gaaataagcg tttgccgcct cgccgacgcc
     3001 gtaaatcgac gcgcccgact gctgcccgag ataaatctgg ttggcataga gcgtgaaaat
     3061 ttcctctttc gtcaggcgcg tttccagaat caccgacata taggcttctt cggctttgcg
     3121 cgtgtatgat ttttccggcg tcaaaaaaag atttttgact aattgctgcg ttatggaaga
     3181 accgccctga cgcgcaatcg gcgagttgtc gtcgccttcg taacgacgcc acagggcgcg
     3241 cagaattccg cgaaagttga cgccgtaatg ctcgaaaaac gcgcg
//
LOCUS       DASWNE010000388         3631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_153934, whole genome shotgun sequence.
ACCESSION   DASWNE010000388 DASWNE010000000
VERSION     DASWNE010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153934"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(277..825)
                     /locus_tag="VGB68_07810"
     CDS             complement(277..825)
                     /locus_tag="VGB68_07810"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016102133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEY0049174.1"
                     /translation="MANELYEIPLKTIDGAETNLGEYKDKVLLIVNVASKCGLTPQYE
                     GLQKLYENYRAQGFEILGFPANNFMGQEPGTEDEIKDFCSTNYEVKFPLFSKISVKGD
                     DRHELYKYLTETKSDTDVNDGGLENNLKGYGHNRSTPAEILWNFEKFLVGKNGEIVAR
                     FAPDVTAEDERLVAKVEEELGK"
     gene            complement(850..1260)
                     /locus_tag="VGB68_07815"
     CDS             complement(850..1260)
                     /locus_tag="VGB68_07815"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEY0049175.1"
                     /translation="MAEIKTKQTEASVEDFFNKIADENVRRDTRKIAELMQKATGEEP
                     KMWGDSIVGFGFRHLKSAAGREVDWLIIGLSPRKQNITLYLSIGEAWNEDLLSRLGKH
                     KTGKGCLYVKRLSDVDENVLQELIEQSAAANTEK"
     gene            complement(1319..1960)
                     /locus_tag="VGB68_07820"
     CDS             complement(1319..1960)
                     /locus_tag="VGB68_07820"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEY0049176.1"
                     /translation="MIQTEIQTYAENHTSDESELLRRLREKTFDERSDKSMLSGFYQG
                     RLLAMFSKMLAPRRVLEIGTYMGYSALCLAEGLSADGKIITLDVDEETNRIAGEFWAQ
                     SEFKDKIESFPGSAPEIIPTLDETFDLVFIDADKPNYANYFDLVFPKLRKGGIILADN
                     VLWSGKVLDIETNADENTLALREFSRKIQADERVSNILFAVRDGLMVIRKETQ"
     gene            complement(2116..3129)
                     /locus_tag="VGB68_07825"
     CDS             complement(2116..3129)
                     /locus_tag="VGB68_07825"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEY0049177.1"
                     /translation="MKAYEIQQFGVENLALVERETPKPAANEVLVKFHAASLNYRDLM
                     MVQGAYNPKLKMPLVPFSDGAGEVAQIGEAVTKWKVGDRVCPIFMQGWIDGEIEFKKA
                     RTALGGDLDGCLREFGAFGENGLVRIPDHLSYEEAATLPCAGLTAYHALFVSGCLKAG
                     ETVLLQGTGGVSIFALQFASVYGSRTIITSSSDEKLERAKGVGADDLINYNETPDWDK
                     AVLDLTEKRGVDHVVEVGGAGTIQKSLKAVKMGGHIALIGALSGGAGGEISPVSVFMK
                     SARLHGIFVGSRQMFEHMNQMLCLHNHLKPVIDKFFEFNEAKDALKYMESGSHFGKIV
                     VRF"
     gene            complement(3268..>3631)
                     /locus_tag="VGB68_07830"
     CDS             complement(3268..>3631)
                     /locus_tag="VGB68_07830"
                     /inference="COORDINATES: protein motif:HMM:NF025946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY0049178.1"
                     /translation="AAQNIPTIEKIAAESPNIETRYVLRDENLELMDKYLTYDARSIP
                     KLVALDAETLEELGTWGPRPQAAMNYFLEMKKNGLEKPQMMENIQRWYLQDKEQSLQK
                     EFEGLLDEWSGKSQAANV"
BASE COUNT          794 a         1016 c          800 g         1021 t
ORIGIN      
        1 aagcttccga ccgaagcgtt tgcgcctgcc gctccaagcc attcattaag tttttcgggg
       61 ctgcgcgtgc cgagctttac ttcgaatcct ttcgccagat agccgctcgc cagagtctgc
      121 gccacctcgc ccgaaccgat aattccgatt ttcatatttt ttaaattatg cacggatgaa
      181 gacgcattga cgcgagataa tttattcgtg aaaattcgtg ttcattcgcg gcttaaatcc
      241 atttcttatc cgcatccgtt cgtctctatc cgcggattat tttcctaatt cttcttcgac
      301 ttttgccact aatcgctcgt cttccgccgt tacgtccggc gcgaaacgcg cgacgatttc
      361 gccgtttttg cccaccagaa acttttcgaa attccacaaa atctcggcgg gcgtcgagcg
      421 gttgtgaccg tagcctttca gattattttc cagcccgccg tcgttaacgt cggtgtcgga
      481 tttggtttcg gtcaaatatt tataaagctc gtgccggtcg tcgcccttga cggaaatttt
      541 tgagaaaagc ggaaatttca cttcgtaatt cgtcgagcag aaatccttga tttcgtcttc
      601 cgttccgggt tcctgtccca taaaattgtt ggcagggaag ccgagaatct cgaaaccttg
      661 agcgcggtaa ttttcgtaaa gcttctgcaa gccttcgtat tgcggcgtca gaccgcattt
      721 cgaggcgacg ttgacgatta ataaaacctt gtctttgtat tcgcccagat tcgtttccgc
      781 gccgtcgatc gttttcagcg gaatttcgta aagttcgttt gccataaaat tttcctcgtc
      841 ctttaaaatt tatttttcgg tgtttgccgc cgccgattgc tcaatcaatt cctgcaaaac
      901 attttcgtcg acgtcgctca gacgcttgac gtataaacag ccttttccgg tcttgtgctt
      961 gccgagccgc gacaataaat cttcgttcca ggcttcgccg attgataaat aaagcgttat
     1021 attctgcttg cgcggcgaaa gcccgattat cagccaatca acttcgcgcc ccgctgccga
     1081 tttcaaatga cgaaagccga aaccgacaat gctgtcgccc cacattttcg gctcttcgcc
     1141 cgtcgccttc tgcattagtt cggcgatttt tcgcgtgtct cgccgcacgt tttcgtcggc
     1201 aattttgtta aaaaaatcct cgacgctcgc ttccgtctgt ttcgtcttta tctccgccat
     1261 aattttctcc gactaaagat ttatttcgac tttaatttct ttgcttccgc cttttgcctt
     1321 actgcgtttc ttttctgata accattaaac cgtcgcgcac ggcgaataaa atattgctga
     1381 ctctttcgtc ggcttgaatt ttgcggctga attcgcggag cgcgagcgtg ttttcgtccg
     1441 cgttcgtttc aatgtccaga acttttccgc tccacaaaac attgtccgcg agaatgattc
     1501 cgcctttgcg cagcttcgga aaaaccaaat cgaaatagtt ggcgtaattc ggtttgtccg
     1561 cgtcgataaa aaccaagtcg aaggtctcgt caagcgtcgg aataatttcc ggcgcgcttc
     1621 ccggaaaaga ctcgattttg tctttaaatt cagattgcgc ccagaattct ccggcaatcc
     1681 ggttcgtttc ctcgtcgacg tcgagcgtga tgattttccc gtccgcgctt aagccttcgg
     1741 ctaaacaaag cgcggaatag cccatataag tcccgatttc cagaacgcgg cgcggcgcga
     1801 gcatcttgct gaacatcgcc agcagccgac cctgataaaa gccggaaagc atcgacttat
     1861 cgctgcgctc gtcgaaagtt ttttcgcgca ggcggcgcag cagttcgctt tcgtccgaag
     1921 tgtgattttc cgcgtaagtt tgtatttccg tctgaatcat aattttaaag tttcggcgtt
     1981 attcgacgga cggcttctgc ctccgaagtc accgccggaa aaacctcgaa ttcgccaata
     2041 tcgctccagt tgacaatcca ttcttccaat aatttttcgg tcgcccgtct ctatcggacg
     2101 accataaaaa gcattttaaa accgcacgac gatcttgccg aaatgcgagc cgctctccat
     2161 atatttcaga gcgtctttcg cttcgttgaa ttcaaaaaac ttatcaatca ccggcttcaa
     2221 atgattgtgc aggcagagca tttgattcat atgctcgaac atctgccgcg agccgacaaa
     2281 aattccgtgc agcctggcgg atttcataaa aaccgagacc gggctgattt cgccgcccgc
     2341 gccgccggaa agcgcgccga taagcgcgat gtgcccgccc attttgacgg ctttcagaga
     2401 tttctgaatc gtgcccgcgc cgccgacttc gacgacgtgg tcaacgccgc gtttttccgt
     2461 taaatccagc acggctttgt cccaatcggg agtttcgttg taattgatta aatcgtcagc
     2521 gcccacgcct ttcgcgcgtt ccagtttctc atcgctcgac gacgtgataa tcgtccgcga
     2581 cccgtaaacg ctggcaaatt gcagtgcgaa aatcgaaacg ccgcccgtgc cctgcagcaa
     2641 gaccgtctcg cccgctttca aacagcccga aacaaacaaa gcgtgataag ccgtcaaacc
     2701 ggcgcacggc aaagtcgccg cttcttcata ggaaagatgg tcgggaattc tcaccaagcc
     2761 gttttcgccg aacgctccga actcgcgcag acagccgtcc aaatcgccgc cgagcgcggt
     2821 tcgagccttt ttaaattcga tttcgccgtc aatccagccc tgcataaaaa tcgggcagac
     2881 gcggtcgccg actttccatt tcgtcaccgc ttcgccgatt tgcgctactt cgcccgcgcc
     2941 gtctgaaaac ggcacgagcg gcattttcaa tttcggattg tatgcgccct gaaccatcat
     3001 caaatcacgg taattcagcg aagccgcgtg aaatttcact aaaacttcgt tagccgcagg
     3061 cttcggcgtt tcacgctcga ccagagccag attttccacg ccgaattgtt gaatctcgta
     3121 tgctttcata ttgattttga attttcgtaa aattcaggat tcaagattca agattcagga
     3181 ttgaaagcaa attcccaatc ctgaatcttg aatcctgaat cctgaattcg gaatcttgaa
     3241 ttccgaatcc ggaatcccga atcgatttca aacattcgcc gcctgcgatt tgcccgacca
     3301 ttcgtccagc aagccttcaa attctttctg caaagattgt tctttgtcct gcaaatacca
     3361 gcgctggatg ttttccatca tctgcggttt ttccagtccg tttttcttca tctctaaaaa
     3421 ataattcatc gccgcctgcg ggcgcggtcc ccacgttccg agttcttcga gcgtttcggc
     3481 gtcgagcgcg accagtttcg gaatcgagcg cgcgtcgtag gtcagatatt tgtccatcag
     3541 ttccagattt tcgtcgcgca agacgtagcg cgtttcgatg ttcgggcttt ccgccgcgat
     3601 tttttcaatc gtcggaatat tctgcgccgc g
//
LOCUS       DASWNE010000389         2086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_260840, whole genome shotgun sequence.
ACCESSION   DASWNE010000389 DASWNE010000000
VERSION     DASWNE010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260840"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VGB68_07835"
     CDS             <1..473
                     /locus_tag="VGB68_07835"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY0049179.1"
                     /translation="INDWNRELALNFAENTLTQQNDKVNVFVVSNDGMASGVLSALAK
                     RKLDGQIFVTGQDAEISALQSIAEGKQSMTVYKPIIPLANGALEAAIKLAKKEPLTDS
                     KPFMNDAIKKEVPSILLEVQTVDKDNLMTTVIKDGYAKFEDVYKNVPAEQRPKQ"
     gene            complement(657..860)
                     /locus_tag="VGB68_07840"
     CDS             complement(657..860)
                     /locus_tag="VGB68_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049180.1"
                     /translation="MENRFELFRELIELSHNERRGLTIFVKGQTIAGGVTKIIGTEAI
                     EIRSQMYSRAIIRIESIDAIAVV"
     gene            complement(1108..1608)
                     /locus_tag="VGB68_07845"
     CDS             complement(1108..1608)
                     /locus_tag="VGB68_07845"
                     /inference="COORDINATES: protein motif:HMM:NF023802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="papain-like cysteine protease family protein"
                     /protein_id="HEY0049181.1"
                     /translation="MFKVAPDVKPLRQAKSTTCWLTCLEMMFQWKNDQGDTSKKKSQI
                     CSKIDTDTDYWSDYLINEGIAPYECYQVARAMGMQPTGAGDYTKEILHDLVSKKGPLW
                     VGGFWYGKSAHVILVTGVDPANGKIKIIDPYNNWDLSEGDRDVGWLNARSKHWKSYEG
                     TVMYWK"
     gene            complement(1835..>2086)
                     /locus_tag="VGB68_07850"
     CDS             complement(1835..>2086)
                     /locus_tag="VGB68_07850"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0049182.1"
                     /translation="NSTDAPADVVRRLKLLSQSWVRRNIPNQNFSWREDYQAFTVSLS
                     QSARVKRYILNQKQHHRQFGAIENYPASWGCVPHHDVCV"
BASE COUNT          523 a          517 c          476 g          570 t
ORIGIN      
        1 acatcaacga ctggaatcgc gagctggcgc tgaacttcgc cgaaaacact ctgacgcagc
       61 aaaacgacaa ggtcaacgtt ttcgtcgtat ccaatgacgg aatggcttcg ggcgtgcttt
      121 cggcgctggc gaaaagaaag ctggacggtc agattttcgt caccggacag gacgccgaga
      181 taagcgcctt gcagtcaatc gccgaaggta aacagtcgat gaccgtttac aaaccgatta
      241 ttcccctggc aaacggcgcg ctcgaagcgg cgataaaact ggcgaaaaaa gagcctctga
      301 ccgactccaa gccttttatg aacgacgcca tcaaaaagga agtgccgtcg attctgctgg
      361 aggttcagac ggtcgataaa gacaatctga tgacgacggt tatcaaagac ggctacgcca
      421 aatttgaaga cgtttacaaa aacgttccgg cagaacagcg tccgaaacag tagttgacgg
      481 gaagttgcag atagttgcgg gtagttgcag gaagttgcgg gtagttaaaa acagtttcgg
      541 agtttcaatc ttcggcagct ttcataactt cctgcaactt cctgtcaact tcctgcaact
      601 tcccgcaaac ttcctgcaac tatgaatccg atttccggat tgcgtcctgc tctgttttag
      661 acgacggcta tcgcgtcgat ggattcgatg cgaataatcg cgcggctata catctggctg
      721 cgaatttcaa tcgcttccgt gccgattatt ttggtcacgc cgcccgctat cgtttgacct
      781 ttgacaaaaa tcgtcaatcc gcgtctttca ttgtggctta attcaatcaa ttccctgaac
      841 aattcaaatc tgttttccat cttcggctcc attgattatt tgatgtctgt gacgagcggc
      901 gaaataccac agcccggttt taaaatattc cttttttctt agtttcatat ttttgttttc
      961 ttagtttcat atttttgcgc cagccgtgat tttctcgccg tcgggctgat agcttcgaat
     1021 aaaattaaaa agagtcgacc attttatatc gcgccaaccc ttttttatga attaagaaaa
     1081 attaaaaata cgagaacaaa tataaaatta tttccagtac attaccgtgc cttcatagct
     1141 tttccagtgt ttgctgcggg cgttgagcca gccgacgtcg cgatcgcctt cggaaaggtc
     1201 ccagttatta taaggatcga tgatttttat cttgccgttt gccgggtcga cgcccgtgac
     1261 gaggatgacg tgcgcgcttt ttccatacca aaagccgccg acccagagcg gacctttttt
     1321 ggaaaccaaa tcgtgcagaa tttccttcgt gtaatcgccc gcgccggtcg gctgcattcc
     1381 catcgcccgc gcgacctgat agcactcgta gggcgcgatt ccttcgttga taagatagtc
     1441 cgaccagtaa tcggtgtccg tatcaatctt gctgcaaatc tgcgatttct ttttgctggt
     1501 gtcgccctgg tcgtttttcc attgaaacat catttccaga cacgtcagcc agcaggtcgt
     1561 cgatttcgcc tgcctcagcg gtttgacatc aggtgctact ttaaacattg ccatgaattt
     1621 tcttctccag ttaaaaaaat aatagttaaa ccctttccct agactgacgg cgattaattg
     1681 ttttgcgttc ttaaaaatga aaatcactac ctaaataggt agtgacgtgc aaagcttcga
     1741 agaaataaaa aatgaagtgt tccggcgcaa atcgcggcgg tcttcgacag taatcgcttt
     1801 gataacttta tttccgtttt tcacaaagct tttactagac gcaaacgtcg tgatgcggca
     1861 cgcagcccca cgacgccggg taattttcaa ttgcgccgaa ctgtcgatga tgctgctttt
     1921 gattgagaat ataacgcttg acgcgcgcgc tttgcgacag gctcaccgta aaagcctgat
     1981 aatcttcccg ccacgaaaaa ttttggttcg gaatattgcg gcgcacccag ctttgcgaaa
     2041 gcagcttgag gcggcgaacg acgtccgccg gcgcgtccgt cgagtt
//
LOCUS       DASWNE010000390         3623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_260846, whole genome shotgun sequence.
ACCESSION   DASWNE010000390 DASWNE010000000
VERSION     DASWNE010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260846"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(296..478)
                     /locus_tag="VGB68_07855"
     CDS             complement(296..478)
                     /locus_tag="VGB68_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049183.1"
                     /translation="MSEETQPIRIETGEEVALELMKFIAEKENKNPSPEEVRDYYLDL
                     FVECAYAVYNGERKTD"
     gene            complement(506..1954)
                     /locus_tag="VGB68_07860"
     CDS             complement(506..1954)
                     /locus_tag="VGB68_07860"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEY0049184.1"
                     /translation="MLKIENYIDGQLIKPVSEAYLDNYNPATGEVYSIIPDSDERDVA
                     LAVEAAKSAFPAWSKMSAEQRHDVLMKIVSLIERDSERLAEAESIDNGKPFSLAKRVD
                     IPRAVSNFKFYATAAMHTATEAHETVGQAINYTLRQPLGVVGCISPWNLPLYLFTWKI
                     APALAAGCTVVAKPSEITPMTAYLLSQICIEAGLPAGVLNIVHGLGAKAGASIVSHKD
                     VKAISFTGGTKTGEWIARTAAPMFKKLSLELGGKNPNVIFADCNYEEMLKTTVLSSFS
                     NQGQICLCGSRVFVERPLYENFCGDFIQRVSALKVGDPLEEKTDVGAIVSQAHMEKIL
                     SYVDLAREEGGHVLHGGKQLKIDGRCAKGYFIEPTIIENLSHDCRTNREEIFGPVVTV
                     TPFDTEDEVLQYANSVEYGLSATVWTENLSRAHRVAEKLESGIIWVNSWLLRDLRTPF
                     GGMKNSGVGREGGFEALKFFTEEKNVCIRINY"
     gene            complement(1988..3214)
                     /locus_tag="VGB68_07865"
     CDS             complement(1988..3214)
                     /locus_tag="VGB68_07865"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEY0049185.1"
                     /translation="MAAKFLIETARKLFALALIFVINTAAFAQTSMPKQTRPRAREAG
                     LKIGILPVGNLNAITDVAGLSIGHTTVIRGENVRTGVTAILPHGSNLFQEKVPGAVFV
                     GNGFGKLAGSTQVNELGEIETPILLTSTLSVPRVGDFLLDYMLALPGNESVQSINPLV
                     AETNDGFLNDIRGRHISREDVFAAIKNAGAGKVEEGSIGAGTGTIAFGFKGGIGTSSR
                     KLPASLGGYTVGVLVQTNFGGVLTIDGAPVGVELGKYYLKDAVEAAQKNNSQTSKTKN
                     DTGDSADGSIIIVIATDAPVDARQLKRMAARSMMGLARTGAAGTNGSGDYAIAFSTAA
                     DVRIKVSDSNPRAVKLLSNEAMSPLFLAVIESTEEAIYNSIFRATTVTGREKRTVEAL
                     PIEKTLAVLRKYGKIK"
     gene            complement(3286..>3623)
                     /locus_tag="VGB68_07870"
     CDS             complement(3286..>3623)
                     /locus_tag="VGB68_07870"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEY0049186.1"
                     /translation="NPPLIAHADGRVEQLASGGFPLGIMPMAQYDVGQARLQKGEALV
                     IYSDGVSEANNTKGEEFGVERLEEVVKKHLGASAAGLRDKVESALSSFTQTAPAGDDI
                     TLVIVKRKS"
BASE COUNT          826 a          969 c          899 g          929 t
ORIGIN      
        1 aacaaaagat ttccgacgcg acgcgcgtgc gggtataaac cgaccggctc cggcgctctg
       61 gaagaattaa taatgttttc gctcatagct aaattaattc gggattcagg attcaggatt
      121 cagtcaggat tcaggattca gaatttaaga tttaagactg gagatttagg atttgccgtt
      181 aaccaaagac caatcctgaa tcctgaatcc tgactgaatc ctgaatctta aattctgaat
      241 cctgaatcct gaatcctgaa tcctgactga atcctgaatc ctgaatcctg aatcttcaat
      301 ccgttttccg ctcgccgtta taaaccgcgt aagcgcattc gacgaataaa tccagataat
      361 aatcgcgcac ttcttcgggc gagggatttt tgttttcctt ttcggcaatg aatttcatca
      421 attcaagcgc cacttcttcg ccggtttcaa ttctaatcgg ttgtgtttct tcactcataa
      481 ttttcctcac gataaatagc aaagctcaat aatttatcct gatgcagacg tttttttctt
      541 cggtaaaaaa tttcagcgcc tcgaaaccgc cttcgcgccc gacgcccgag tttttcattc
      601 cgccgaaagg cgtgcgcaaa tcacgcagaa gccacgaatt tacccagata atgcccgatt
      661 ccagtttttc ggcaacgcga tgcgcgcggc ttaaattttc cgtccagacg gtcgccgaca
      721 agccgtattc gacgctgttg gcgtactgca aaacttcgtc ttccgtgtcg aaaggcgtga
      781 cggtgacgac cggaccgaaa atttcttccc ggttcgtgcg gcagtcgtgg gaaagatttt
      841 cgattatcgt cggctcgata aaatagcctt tggcgcatct gccgtcgatt ttcaattgtt
      901 ttccgccgtg cagaacgtgc ccgccttctt cgcgcgccaa atcgacgtaa gaaagaattt
      961 tttccatatg cgcctgcgaa acgattgcgc cgacatcggt tttttcttcg agcgggtcgc
     1021 cgacttttaa ggcggagacg cgctggataa aatcgccgca aaaattttcg taaagcgggc
     1081 gctcgacaaa aacgcgcgag ccgcagaggc agatttgccc ttgattcgaa aaggacgaga
     1141 gcaccgtcgt tttgagcatt tcctcgtaat tgcagtcggc gaaaatgacg ttcggatttt
     1201 tgccgccgag ttcgagcgag agttttttga acatcggcgc ggcggtgcgc gcaatccatt
     1261 cgccggtttt cgtgccgccg gtaaaggaaa tcgctttcac gtccttgtgc gagacaatcg
     1321 acgcgcccgc ttttgcgccc agaccgtgga cgatgtttaa aacgcccgcg ggcaaaccgg
     1381 cttcgatgca gatttgcgac aaaagataag ccgtcatcgg cgtaatttcc gaaggctttg
     1441 ccacgaccgt gcagcctgcg gcgagcgcgg gcgcgatttt ccaggtgaat aaataaagcg
     1501 gcaggttcca gggacttatg cagccgacga cgccgagcgg ctggcgcaga gtgtaattta
     1561 tcgcctgccc gaccgtctcg tgcgcttcgg tcgccgtgtg catcgcggcg gtcgcgtaaa
     1621 atttgaaatt ggaaacggcg cgcggaatat ctactctttt cgccagcgaa aagggctttc
     1681 cgttgtcgat gctttcggct tcggcgagcc gttccgaatc tctctcaatc aaggacacga
     1741 ttttcatcaa aacgtcgtgt ctctgttccg cgctcatttt cgaccaagcc ggaaaagcgc
     1801 ttttcgccgc ttcgaccgct aaagcgacgt ccctttcatc cgaatcggga attatcgaat
     1861 aaacttcgcc cgtggcggga ttataattat ccagataagc ttccgaaacg ggtttaatca
     1921 attgaccgtc gatgtaattt tctattttga gcattctgaa aaaggattat actgcatttc
     1981 gcagccttta tttaatcttt ccgtatttac gcaggacggc gagcgttttt tcaatcggca
     2041 gggcttcaac cgtgcgtttt tcgcgtccgg tgacggtcgt ggcgcgaaaa atcgaattgt
     2101 aaatcgcttc ttcggtcgat tcgatgaccg ccagaaaaag cggcgacatc gcctcattgg
     2161 acagcaattt gacggctcgc ggattagagt cggaaacttt gatacgaacg tcggcggcgg
     2221 tcgaaaaagc aatcgcgtaa tcgccgcttc cgtttgttcc ggcggcgccc gttcgcgcca
     2281 gccccatcat cgagcgagcc gccattcgct ttaactgccg cgcatcgacg ggcgcatcgg
     2341 tggcaatcac gataatgatt gaaccgtcag ccgaatcgcc ggtatcattt tttgttttgg
     2401 aagtttgaga attgtttttt tgagcggctt caaccgcatc ttttaaatag tattttccca
     2461 gctcgacgcc gaccggcgcg ccgtcaatcg tcagaacacc gccgaaattc gtctgaacca
     2521 gcacgccgac cgtgtagccg cccagagatg ccggaagctt tctcgaagac gtgccgattc
     2581 cgcctttgaa accgaaagcg atggttcccg ttcccgcgcc gatcgagcct tcttcgactt
     2641 ttcccgcgcc cgcgtttttg attgccgcga aaacgtcttc acggctgata tgacgaccgc
     2701 gaatgtcatt cagaaaaccg tcattggttt cggcaaccag cggattaatg gactgaaccg
     2761 attcattgcc cggcaaagcc agcatataat ccaacaaaaa atcgccgacg cgcggaacgc
     2821 tcaatgtgga agtcagaaga atcggagttt cgatttcgcc gagctcattg acctgcgtcg
     2881 accccgccag tttgccgaag ccgttgccga cgaaaaccgc gccgggaact ttttcctgaa
     2941 agagattgct gccgtgcggc agaatagccg tcacgccggt tctgacgttt tcaccgcgaa
     3001 taactgttgt atgaccaata cttaagcccg caacgtcggt tatcgcatta agatttccga
     3061 cgggcaaaat gccgattttc agtccggctt cgcgggcgcg cggtcttgtc tgttttggca
     3121 tcgatgtttg agcgaaagcc gccgtattaa taacaaaaat cagcgccagc gcgaagagtt
     3181 tgcgagccgt ttcaattaaa aattttgcag ccatttttcc ggtatgaaaa aacagcttta
     3241 cagatttttg caaagctgtt ttttactaac aatttgcatt ttttcctagc tctttctttt
     3301 gacaatcacc aaagtaatat cgtcgcccgc cggcgcggtt tgggtgaatg aggaaagcgc
     3361 cgattcgact ttgtcgcgca agcccgccgc cgatgcgccg agatgttttt tgacgacttc
     3421 ttccaggcgc tcgacgccga attcttcgcc tttcgtgttg tttgcctccg agacgccgtc
     3481 cgaataaatc accagcgctt cgcccttttg caggcgcgcc tgcccgacat cgtattgcgc
     3541 catcggcata atgccgagcg ggaatccgcc cgaagcgagc tgctcgacgc gcccgtcggc
     3601 gtgggcgata agcggcggat tat
//
LOCUS       DASWNE010000391         5126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_296241, whole genome shotgun sequence.
ACCESSION   DASWNE010000391 DASWNE010000000
VERSION     DASWNE010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296241"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..1943)
                     /gene="asnB"
                     /locus_tag="VGB68_07875"
     CDS             complement(12..1943)
                     /gene="asnB"
                     /locus_tag="VGB68_07875"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEY0049187.1"
                     /translation="MCGINGIAFSSGKRRVDESTLRRMRDVIHHRGPDDGGVFVEANV
                     GLGHRRLSIVDVSHGAQPMFNEDASVCIVYNGEVYNHADYRAELEAKNHVYKTHCDTE
                     TILHLYEEYGSDCVEKLRGMFAFAIWDRRKKELFIARDRLGVKPLYYVHDAEGNLFFA
                     SEIKSLLEAKAVRAEINFNVLPDQLANHGTSGEETLYSGVKRLAPGHTLIWKDGKINV
                     RKYWDVSFEPKHENKPDAEFVDEWRELFRKSVELRLMADVPLGMFLSGGIDSSAICAM
                     MARTVDAPVKTFSVGFSEREANELAYARLVADAFQTDHHEITVTPEQFFEALPRLIWH
                     EDEPLGFIASVPLYFISKLAQKHVKVVLTGEGSDEILGGYGRYRKTLALLKYGAKYES
                     LTPDFLRAFVKNNLGNLPSSGKLKRTFLSRPADMENLYFDNFAIFSKAMQENLLSAET
                     KEKIAERNPYADLQNHLLQTDAEDLLDKLLYADTKTYLHELLMKQDQMSMAASIESRV
                     PFLDHKLVEYTAKLPQKMKIRGRETKWILREAMRGILPAEILNRPKMGFPVPVGRWFR
                     NEFRGIVEEYVSSERASSRGIFNAGYVREIVARHNAGENHDERLWFLVNFEMWQRQFI
                     DGEIPENKESELAKEFATI"
     gene            complement(1979..3130)
                     /locus_tag="VGB68_07880"
     CDS             complement(1979..3130)
                     /locus_tag="VGB68_07880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0049188.1"
                     /translation="MHILLLGPYPPPQGGVQRNMLAIRDELRRAGHACSIINITRSAQ
                     AVPEPDVYHPQTPLALARLLLKLKYDLLHIHIGGEIPARVLGLLALCALAARGKSVLT
                     LHSGGYPLSEDGRAARKFSLRGFIFRFYKRLVCVNRQQVEMFEKFGAAKEKIRLIYPF
                     FNQNPNENVSIPAALESFVAAHQPFLLTVGLLQPTYNLPLQIKTLGKIREKFPGAGLM
                     IAGAGELEDELRRIIAAAPDGEHILLAGDVEHAVTLRLINECDVLLRTTDYDGDAISV
                     REALHLDTPVVATDNGMRPEGVYLIPKGDGAALEKAIESTLGKGAKIKSVKPDDYRNI
                     TAILNLYEEINGAQSESSREKTLPSKVSSLWLFWLVLNALRDLLGSGAA"
     gene            complement(3274..>5126)
                     /locus_tag="VGB68_07885"
     CDS             complement(3274..>5126)
                     /locus_tag="VGB68_07885"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HEY0049189.1"
                     /translation="IEKHASVTDLEIGQRVAYGGEGTGHGETINVGRNLIARVPGDVD
                     FQSACFTTLGAIAMNAVRLAEIGVGETVAVIGLGIVGQLVSQLVRCQGGIPLAIDLQP
                     DRVDLARKTGADFGLIAGDKTVAEVKALTNGRGADCVIVAAASKSAKPLEQAAQMSRD
                     RGRIVVVGAVPMEMPRGEMYIKELKVLMARAYGPGSYDASYEKRGIDYPLPYVRWTEN
                     RNMEEFLRLISVGRVDVKPLISHEYALEDAPRAYETIMRGNGKASLAVVLKYPVNDFE
                     NPIESYQPKRKISVNDYQPKTGEINFALVGAGNLAKWAHLPALKKISGARLHAVYSGH
                     GARGKSYAMRFGATYHTSEFEEILNDKTVDVVLISSRHKEHAEQAIAALNAGKHVFIE
                     KPMAITMEECREIYRAVKTSGKQLAVGFNRRYAPFYAEMKKQLKNRTGAAVVSTRMNS
                     PGIENGWAAESGQGGVVLGEGCHFVDLMYWLLDSEPVSVSAYGFDEHNVAASLKFADG
                     SIGNFIYTVVGSESSGGELVEAFAPGVAATTEDFKRLTVKKKTAKTNSRWFAEKGYAA
                     QLESFVQSLRDGRETEVTVRDGARATLGCLLMLESVKTGLPCEFNLDEVL"
BASE COUNT         1097 a         1487 c         1312 g         1230 t
ORIGIN      
        1 atcaacataa atcagattgt ggcaaactct ttcgccaatt cgctttcttt attttccggt
       61 atttcgccgt cgataaactg ccgctgccac atctcgaaat taaccagaaa ccacaatctt
      121 tcgtcgtgat tttcgcccgc gttgtggcgc gcgacgattt cgcgcacgta accggcatta
      181 aaaattccgc gcgacgaggc gcgctcgctc gaaacgtatt cttcgacaat gccgcgaaac
      241 tcgtttctga accatctgcc gaccggaacg ggaaaaccca ttttcgggcg atttaaaatt
      301 tcggcgggca aaattccccg catcgcctcg cgcagaatcc atttcgtctc gcgcccgcga
      361 atcttcattt tttgcggcaa ttttgccgtg tattccacca gcttgtggtc gagaaacggc
      421 acgcgcgatt ctatcgaagc cgccatcgac atctggtcct gcttcatcaa aagctcgtgc
      481 agataagttt tcgtgtcggc gtacagcagc ttgtcgagca aatcttcggc gtccgtctgc
      541 aagagatgat tttgcaaatc cgcgtacgga tttcgctccg cgattttttc tttcgtttcg
      601 gcgcttaaaa gattttcctg catcgccttt gaaaaaatgg cgaaattatc gaaatacaaa
      661 ttttccatat cagccgggcg cgacaggaac gtgcgtttta attttccgct tgacggcaga
      721 tttccgagat tgttttttac aaacgcgcgc aaaaaatccg gcgtcagcga ttcgtatttc
      781 gcgccgtatt tcaagagcgc gagagttttc cggtagcgac cgtaaccgcc taagatttcg
      841 tcgctgcctt cgcccgtcag gacgacttta acgtgttttt gcgccagttt cgagatgaaa
      901 taaagcggaa ccgaggcgat gaaaccgagc ggctcgtctt cgtgccagat taggcgcggc
      961 aaagcttcaa aaaattgttc gggcgtgacc gtgatttcgt gatggtcggt ttggaaagcg
     1021 tccgccacga gccgcgcgta ggcaagctcg ttggcttcgc gctcgctgaa tccgaccgag
     1081 aaagttttaa cgggcgcgtc gacagtgcgc gccatcatcg cgcaaatcgc ggacgagtcg
     1141 atgccgcccg acaaaaacat tccgagcgga acgtctgcca tcaggcgcag ctcgaccgat
     1201 tttcggaaaa gctcgcgcca ctcgtcgaca aattcggcgt ccggtttgtt ttcgtgtttc
     1261 ggctcgaagg aaacgtccca gtatttccgg acattgattt tcccgtcttt ccaaatcagc
     1321 gtgtgaccgg gcgcgagacg cttgacgccg ctgtataaag tttcctcgcc ggaagttccg
     1381 tgattcgcca gctgatcggg caaaacattg aaatttattt ccgcgcggac ggctttcgct
     1441 tccagcaggc ttttgatttc cgaagcgaaa aacaaatttc cttccgcgtc gtgaacgtaa
     1501 taaagcggct tgacgccgag gcggtcgcgc gcgataaaca attctttttt gcgccggtcc
     1561 caaatcgcga aggcgaacat tccgcgcaat ttttcgacgc aatccgagcc gtattcctcg
     1621 tagagatgca gaatcgtttc cgtatcgcag tgagttttat aaacgtgatt tttggcttcc
     1681 agctcggcgc ggtaatcggc gtgattgtaa acctcgccgt tgtagacgat gcaaacggac
     1741 gcgtcttcgt taaacatcgg ctgcgcgccg tgcgaaacat cgacgatcga aagccgccgg
     1801 tgcccgagcc cgacgtttgc ctcgacgaaa acgccgccgt cgtccggtcc gcgatgatga
     1861 atcacgtcgc gcatccggcg cagcgtcgat tcgtcgacgc gccgcttccc gcttgaaaat
     1921 gctattccgt tgattccgca cataaaattc gtttagcctg ctccgcgttt ccgatttttc
     1981 aagccgcgcc gcttcccaat aaatcccgta aagcattcaa tacaagccag aacagccaca
     2041 gcgacgagac cttcgacggc aaggtttttt cgcgcgagct ttcgctttgc gcgccgttta
     2101 tctcttcata gagatttaaa atcgccgtga tattccggta atcgtccggc ttgacggatt
     2161 ttattttcgc gcctttcccg agcgtcgatt ctatcgcttt ttccagcgcc gcgccatcgc
     2221 ctttcggtat cagataaacg ccttcgggac gcattccgtt gtcggtggcg acgaccggcg
     2281 tgtccagatg cagcgcctcg cggacggaaa tcgcgtcgcc gtcgtaatcg gtcgtgcgga
     2341 gcaaaacgtc gcactcattt attaaacgaa gcgtcacggc gtgttcgaca tcgcccgcca
     2401 gcaaaatatg ctcgccgtcg ggcgcggcgg cgatgattcg gcgcagctcg tcttccagct
     2461 cgcccgcgcc ggcaatcatt aaacccgcgc cgggaaattt ttccctgatt tttcctaacg
     2521 tcttaatctg caaaggcaga ttataggtcg gctgaagcag cccgaccgtc agcaaaaaag
     2581 gctggtgcgc ggcgacaaag ctttcgagcg cggcgggtat gctgacgttt tcgttcggat
     2641 tctgattaaa aaacgggtaa atcaggcgaa ttttttcttt cgccgcgccg aatttttcaa
     2701 acatttcgac ctgctggcga ttgacgcaga ccagacgctt gtaaaaccgg aagataaagc
     2761 cgcgcagaga aaatttccgc gccgcgcgcc cgtcttccga taaaggatag ccgccggaat
     2821 ggagcgtcaa aacgcttttt cctctcgccg ccagcgcgca gagcgccagc agtccgagca
     2881 cgcgcgccgg aatttcgccg ccgatatgaa tgtgaagcaa atcgtatttt aatttgagca
     2941 gcagccgcgc cagcgccagc ggcgtctgcg gatgataaac gtccggctcg ggcacagcct
     3001 gcgcgctgcg cgtgatgttg ataatcgagc acgcgtgtcc ggcgcggcgc aattcgtcgc
     3061 gtatcgccag catattgcgc tgaactccgc cctgcggcgg cggatatggt cccagaagca
     3121 gaatgtgcat ttttaaaatc aagcgatgag cgccgaacga tgaatgacga acaaaagaaa
     3181 gttttaggtt ttgaatttta agctgccgaa aatcattaat tcaaaactca aaattcataa
     3241 tcaattcatc gttcatcgtt cggcgtttat cgtttataaa acctcatcca aattaaactc
     3301 gcagggcaag cccgttttca ccgattccag catcagcagg cagccgagcg tggcgcgcgc
     3361 gccgtcgcgg acggtgactt cggtttctct tccgtcccgc aaagattgga cgaaagattc
     3421 gagctgcgcc gcgtagcctt tttcggcaaa ccatcgcgaa tttgttttcg cggtcttttt
     3481 ctttaccgtc aggcgtttga aatcttccgt cgtcgccgcg acgccgggcg caaaggcttc
     3541 gaccagctcg ccgcccgaag attcgcttcc gacgaccgtg taaataaaat tcccgattga
     3601 gccgtcggca aacttcagcg aagcggcgac attgtgctcg tcaaagccgt aagccgaaac
     3661 cgaaaccggc tcggaatcca gaagccagta catcaaatcg acaaagtgac agccttcgcc
     3721 gaggacgacg ccgccctgac cgctttccgc cgcccagccg ttttcgatgc ccggcgaatt
     3781 cattctggtc gaaacgaccg ccgcgcccgt gcggtttttc agctgctttt tcatctcggc
     3841 gtaaaacggc gcgtagcggc gattaaagcc gacggcgagc tgtttgccgc tcgttttgac
     3901 ggctcgataa atttcgcggc attcttccat cgtaatcgcc atcggctttt cgataaagac
     3961 gtgctttccg gcgttgagcg ccgcgatcgc ctgttcggcg tgttctttat gacgactgga
     4021 aatcagaacc acgtcaaccg ttttatcatt taaaatttct tcgaattccg acgtgtgata
     4081 cgtcgcgccg aaacgcatcg cgtaggattt tccgcgcgcg ccgtgaccgg aataaaccgc
     4141 gtgcaggcgc gcgccgctaa tctttttcag cgcgggcaga tgcgcccatt ttgccagatt
     4201 tcccgcgccg accagcgcga aatttatctc gcccgttttc ggctggtaat cgttgacgct
     4261 gatttttcgt ttcggctggt aagattcgat tggattttcg aaatcgttga ccggatattt
     4321 taaaacgacg gcgaggctcg ccttgccgtt gccgcgcata atcgtttcgt aagcgcgcgg
     4381 cgcgtcttcg agagcgtatt cgtggctgat taaaggcttg acatcgactc tgccgacgga
     4441 aatcaggcgc agaaattctt ccatattgcg gttttccgtc cagcgcacat acggcagcgg
     4501 ataatcgatt ccgcgctttt cgtaactcgc gtcgtagctg cccggaccgt atgcgcgcgc
     4561 catcaaaact ttcagctcct tgatatacat ctcgccgcgc ggcatttcca tcgggacggc
     4621 tccgaccaca acgattcgcc cgcggtcgcg cgacatctgc gcggcttgtt ccagaggctt
     4681 cgccgatttc gacgccgccg cgacgataac gcagtccgcg ccgcgcccgt tcgttaaagc
     4741 tttcacttcg gcgaccgttt tatcgcccgc gatcaagccg aaatccgcgc cggttttccg
     4801 cgccaaatca actctgtcgg gctgcaaatc aatcgccagc ggaattccgc cctggcagcg
     4861 aacgagctgc gagaccaatt gcccgacgat tcccaatccg ataaccgcga ccgtctcgcc
     4921 gacgccgatt tccgccaatc gaaccgcatt catcgcaatt gcgccgagcg tcgtaaaaca
     4981 cgccgattga aaatcgacgt cgccgggcac gcgcgcgatt aaatttcgcc cgacgtttat
     5041 cgtctcgccg tgtcccgtgc cttcgccgcc gtaagcgacg cgctgaccga tttccaaatc
     5101 cgtcacgctg gcgtgttttt caatga
//
LOCUS       DASWNE010000392         3261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_13260, whole genome shotgun sequence.
ACCESSION   DASWNE010000392 DASWNE010000000
VERSION     DASWNE010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13260"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="VGB68_07890"
     CDS             <1..982
                     /locus_tag="VGB68_07890"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0049190.1"
                     /translation="IGAFEEALKMRGIDKTALATEEESEFANRVFGKIISTNKNANRY
                     TEAKAAIDRARLVFDKEDLFADRQLTSMYLETGKRQEALQVIRGMRLRYPSDESLVRL
                     EATVLTDLGKVDEGVTLIRNLLKGKTASAPSALSDDFSNYIFISMLYTQGKRGKEAIE
                     AANLAFNAAESDERKQIAKLNLATAQHETGDFAAAETTLRDILKQTPRNPIALNNLGY
                     FILERNERFDEALRLIQQAVRIDPTNPSYLDSLGWAYFKLGKFDEAEKYLKSAVRRSS
                     DSATIYEHLGDVYHKQNKTDLAKIAWQKALNLASGAEETNRIKTKLGRKK"
     gene            1051..1965
                     /gene="ispE"
                     /locus_tag="VGB68_07895"
     CDS             1051..1965
                     /gene="ispE"
                     /locus_tag="VGB68_07895"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEY0049191.1"
                     /translation="MDSFTLPAFAKINWFLRVLGKRDDRFHEICTAFQTISLHDKLTF
                     SETEEFVLTCDQPDIPVDERNLIFRAGVALREKFEIKKGASIHLEKRIPAPGGLGGGS
                     SDAAIALLGLAKLWQIEIKFEELCEIGARLGSDVPFFFFGGTAFGRGRGTEIFPVDEL
                     EEKFMLVVAPKIDVPTAEAFAGLKALRLTNQSTESILKHCRSEAEKLNLRQSVLINDF
                     EPSVFKIQPEIKRVKEKLLELGASRALLSGSGASVFAVFDKQETRQTTLKALEDRHNW
                     RMFAVATISRNKYREALEMSEKLFPISF"
     gene            1982..2056
                     /locus_tag="VGB68_07900"
     tRNA            1982..2056
                     /locus_tag="VGB68_07900"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2014..2016,aa:Gln,seq:ttg)
     gene            2233..3189
                     /locus_tag="VGB68_07905"
     CDS             2233..3189
                     /locus_tag="VGB68_07905"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEY0049192.1"
                     /translation="MSVNGNVKIFSGNAHPSLTEEICRHLSCDLGMANTTRFSDGEYN
                     FHIQENVRGADVFIIQPTCPPTDQNLMELLIMIDTFVRASAERVTAVIPYFGYARSDK
                     KDRPRVPIAAKLVANLITTAGAHRIMTIDLHAAQIQGFFDIPVDHIYAAPVMVKYFQD
                     NPLDDLVVVAPDTGGAERARAYAKRLDAGLALVDKRREKANVAEVMNVVGEVEGKNCV
                     IIDDMCDTGGTICKVADAIYREGAKRIFAGFTHAVLSGCAAENIRNSHIEKVIVTDTI
                     PLVGGAKELEAEGRIVVMSVARLLAKGIKSIHDETSVSSLFI"
BASE COUNT          866 a          772 c          889 g          734 t
ORIGIN      
        1 gatcggcgct tttgaagaag ccctgaaaat gcgcggcatt gataaaacgg cgctggcgac
       61 cgaagaggaa agcgaatttg cgaatcgggt tttcggcaaa attatctcga ccaacaaaaa
      121 cgcgaatcgt tacaccgaag ccaaagccgc gattgaccgc gcgcgtctgg tttttgacaa
      181 agaagattta tttgccgacc gccaattgac ttcgatgtat ctggaaaccg gaaagcggca
      241 ggaagcctta caggttattc gaggaatgcg cctgcgttat ccgtcagatg aaagcctcgt
      301 ccgtctggaa gcgacggttt tgaccgattt gggaaaagtc gacgaaggcg tgacgcttat
      361 ccggaatctg ctgaaaggca aaaccgcgag cgcgccgtcg gctctgtcgg atgatttcag
      421 taattatatt tttatttcga tgctttacac gcagggcaaa cgcggaaaag aagcgattga
      481 agcggctaat ctggcgttta acgcggcgga aagcgacgag agaaaacaaa tcgcgaaatt
      541 gaacctggcg accgcgcagc acgaaaccgg cgatttcgcc gccgccgaaa cgacgctgcg
      601 cgacattctg aagcagacgc cgcgcaatcc gattgccctg aacaatctgg gttattttat
      661 tctggagcgc aacgaaagat ttgacgaagc cctcagactg attcagcagg cggtcaggat
      721 tgacccgacc aatccgtctt atctggacag cttaggctgg gcttatttca agctcggaaa
      781 atttgacgaa gcggaaaaat atctgaaaag cgcggtgcgg cgcagttccg attcggcgac
      841 gatttacgaa catctcggcg acgtctacca caaacaaaat aaaacggatt tggcgaaaat
      901 cgcctggcag aaagccttaa atctcgcctc gggcgcggaa gagacaaacc gcatcaaaac
      961 caaattggga aggaagaagt gataactggc agttgataac tgaaaatagc cgtcagttat
     1021 caactaccag ctaccagtta attttacgtg atggattcat tcacgcttcc ggcgtttgcc
     1081 aagataaact ggtttctgcg cgtgctcggc aaacgcgacg accggtttca cgaaatctgc
     1141 acggcgtttc agacaatttc cctgcacgat aaactgacgt tttcggaaac ggaagagttt
     1201 gttttgacct gcgaccagcc ggatattccg gtcgacgaga gaaatttgat ttttcgggcg
     1261 ggcgtggctt tacgggaaaa attcgaaata aaaaagggcg catccattca tctggaaaaa
     1321 cggattcccg cgccgggcgg actcggcggc ggctcaagcg acgcggcaat cgcgctgctc
     1381 ggtctggcaa agctctggca aatcgaaatt aaatttgaag agctgtgcga aatcggcgcc
     1441 cggctcggat ctgacgtgcc gtttttcttt ttcggcggga cggcttttgg tcgcgggcgc
     1501 ggcacggaaa tttttccggt cgatgagctg gaagaaaaat ttatgctcgt cgtcgcgccg
     1561 aaaatcgacg tgccgacagc cgaagctttc gccggtttaa aggcgcttcg cttgacaaat
     1621 caatcgacgg aaagtattct caaacattgc cgtagtgaag ccgaaaagct gaatttacgg
     1681 caatctgttt taataaacga ttttgagccg agcgttttca aaatccagcc cgaaatcaaa
     1741 cgcgtgaaag aaaaacttct ggaactcggc gccagccgcg cgttgctgtc gggcagcggc
     1801 gcaagcgttt tcgcagtctt tgacaaacaa gaaacacggc aaacgacatt aaaagccctt
     1861 gaagacagac acaactggcg aatgtttgcc gtagcaacta tttcgcggaa taaataccgc
     1921 gaagcactcg aaatgagtga aaagttgttt ccgattagtt tctaaaacaa taatttcctg
     1981 ttggggcgta gccaagtggt aaggcaccgg tctttggatc cggcattcgt aggttcgagt
     2041 cctcccgccc cagccatgtt tttattttaa actgacggac aactctcgat tgggttgtcc
     2101 tttttatttt cgattttatt aatcggcttt cggttttcgg tctttggtct tcgttaaaga
     2161 attacgctcc ggcatcgtgc ggaagaaatc gaagtccgaa agccgaagac caaagaccgg
     2221 tttaaaagtc caatgagcgt caacggtaac gtcaaaattt tttcaggcaa tgcgcatccg
     2281 agtttaaccg aggaaatttg ccggcatctc agttgtgatt tgggaatggc aaacacgacg
     2341 cggttttccg acggcgaata taattttcat attcaggaaa acgtgcgcgg cgcggatgtt
     2401 ttcataattc agccgacctg tccgccgacc gaccagaatt tgatggagct gctgattatg
     2461 attgacactt tcgtgcgggc ttcggcggag cgcgtgacgg ctgtaattcc atatttcggt
     2521 tacgcgcgct cggacaaaaa agaccgaccg cgcgtgccga ttgccgccaa gctggtggcg
     2581 aatctgataa cgaccgcggg cgcgcaccgg attatgacga ttgatttgca cgcggcgcag
     2641 attcaagggt tttttgatat tccggtcgac catatttacg ccgcgccggt gatggtcaaa
     2701 tattttcagg acaatccgct ggacgatttg gtcgtcgtcg cgccggatac gggcggcgcc
     2761 gaacgcgccc gggcttacgc caaaagatta gacgccggat tggcgctggt cgataaacgc
     2821 cgcgagaagg cgaatgtcgc cgaagtgatg aacgtcgtcg gcgaggtcga aggcaaaaac
     2881 tgcgtgataa tcgacgatat gtgcgatacg ggcggcacga tttgcaaagt cgccgacgcg
     2941 atttacagag aaggcgcgaa aagaattttt gcgggtttta cgcacgccgt tttatcgggc
     3001 tgcgcggcgg agaacatccg caattcgcat atcgaaaagg taatcgttac cgacacgatt
     3061 ccgctcgtcg gcggagccaa agagcttgaa gccgaaggca gaatcgtcgt gatgagcgtg
     3121 gctagattgt tagccaaggg aattaaatca atccacgatg aaacgagcgt ttcgtcgttg
     3181 tttatttaaa aaagcggtca gcagtcagca gtcagcagtc agcaggaaaa atccgaagac
     3241 cgaagaccga agaccgaaga c
//
LOCUS       DASWNE010000393         2820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_365377, whole genome shotgun sequence.
ACCESSION   DASWNE010000393 DASWNE010000000
VERSION     DASWNE010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365377"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VGB68_07910"
     CDS             complement(<1..488)
                     /locus_tag="VGB68_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049193.1"
                     /translation="MIQRKVLNTFAVLLFALFFWSADAFGKDEWLSVRSKNFNLIGNA
                     SEKDIRTAAARLEQFREVFRQIFDKVNFNSPVPTTVVVFKDANSYNPYKPIKADGQID
                     KAVAGYFLSADDANFITLSMEGDTNQAYETIFHEYTHFWVANNVGKSNVPPWFNEGLA
                     EY"
     gene            608..766
                     /locus_tag="VGB68_07915"
     CDS             608..766
                     /locus_tag="VGB68_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049194.1"
                     /translation="MQNRAEIYFIAAMMILILVGCAVACVAFFKTYKKEKAQREKQIA
                     DRKSKIEN"
     gene            769..1326
                     /locus_tag="VGB68_07920"
     CDS             769..1326
                     /locus_tag="VGB68_07920"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEY0049195.1"
                     /translation="MSHPAVLDKQQTVLVVVDMQEAFRKAIPAFAEIASRVAQMIRGC
                     RILDVPILVTEQYPKGLGKTAEEIIFSLPDDFEFIEKTAFSSCGAPSFMEKLETAQAK
                     QILLCGIETHICVNQTTHDLLENGYQVHLLTDCVGSRFEQDKLAALQKLTTNGAFPSS
                     LEMALFELLRDSGHEQFKEIQNLIK"
     gene            complement(1319..1729)
                     /locus_tag="VGB68_07925"
     CDS             complement(1319..1729)
                     /locus_tag="VGB68_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049196.1"
                     /translation="MTANVDEQKYGKLLMQTLPSVITSDEELERLTKEVDRLMTKGIK
                     EDELSPEEEKLLELLAVLIEQYEDEHYPMPDIPPHEVLKYLMKENGYKQKDIVHIFGS
                     SGITSEVVNGKRSISKAQAKKLAEFFSVSVELFI"
     gene            2207..>2820
                     /locus_tag="VGB68_07930"
     CDS             2207..>2820
                     /locus_tag="VGB68_07930"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY0049197.1"
                     /translation="MDILSSLNPQQQEAVQTTEGALLILAGAGSGKTRVITVRIAYLI
                     AEKGVAPYNILAVTFTNKAAGEMRERVESLLKGRGLQSAPLISTFHSLCVRILRQDIE
                     HLSEGYSKSFTIYDTDDSMKVIKACVKDIGLDDKQLPARQVQSAISASKNRGEDFEDY
                     ASRIEYTDERRAAIARVFKMYEERLNNSNALDFDDLLIKTVRLLR"
BASE COUNT          724 a          690 c          629 g          777 t
ORIGIN      
        1 taatattccg ccaagccttc gttaaaccac ggcggcacgt tggatttccc gacgttgttg
       61 gcgacccaga aatgcgtgta ttcgtgaaaa atcgtttcgt aggcttgatt ggtgtcgcct
      121 tccatcgaga gcgttatgaa attggcgtcg tcggcagaca gaaaatagcc ggcgaccgct
      181 ttgtcgattt gcccgtcggc tttaattggt ttatacggat tataagaatt cgcgtccttg
      241 aaaacgacga cggtcgtcgg aaccggcgaa ttaaaattca ccttgtcgaa aatctgccgg
      301 aaaacttcgc gaaattgttc gaggcgcgcc gctgccgtgc gaatgtcttt ttcgctcgcg
      361 tttccgatca aattaaaatt tttcgagcgc accgagagcc attcgtcctt cccgaaagcg
      421 tcggcgctcc agaaaaaaag ggcaaacaaa agcactgcaa acgtgttgag aactttgcgc
      481 tgaatcataa acttacctgt aaatttttgc ttgagatttc cgaaactttt taccgcgttt
      541 ttcaatatct ctttgacgat ttatagcgtc tttgcgttgc atccttgata cggttaaact
      601 tatctttatg cagaatcgag cggaaattta ttttatcgcg gcgatgatga ttttaatcct
      661 cgtcggctgc gccgtcgcct gcgtcgcttt ttttaaaaca tacaaaaaag aaaaagccca
      721 gcgcgaaaaa caaatcgccg accgaaagtc gaaaatcgaa aattaaatat gtcgcatcca
      781 gccgttttag acaaacagca aaccgtttta gtggtcgtcg atatgcagga agcgtttcgc
      841 aaagcgattc ccgcttttgc cgaaatcgcc tcgcgcgtcg cgcagatgat tcgcggctgc
      901 cggattctgg acgtgccgat cctcgtcacc gaacaatatc cgaaaggctt gggcaaaacg
      961 gcggaagaaa ttattttttc attgcccgac gatttcgagt tcatcgaaaa aaccgcgttc
     1021 agctcgtgcg gcgcgccttc ctttatggaa aagctggaaa cggcgcaggc taaacaaatc
     1081 ctgctctgcg gcatcgagac gcacatctgc gtcaaccaaa cgacgcacga ccttctcgaa
     1141 aacggctatc aggttcacct tttaaccgat tgcgtcggct cgcgcttcga acaagacaag
     1201 ctggcggcgc ttcagaaatt gacgacgaac ggcgcttttc cgtccagcct cgagatggcg
     1261 cttttcgagc tgctgcgcga ttccggacac gagcagttta aggaaattca gaatcttatt
     1321 aaataaaaag ctcgaccgaa acactaaaaa actccgccag ctttttcgcc tgcgccttgc
     1381 tgatcgagcg cttgccgttg acgacttcgg aagtaattcc gcttgagccg aaaatgtgaa
     1441 cgatgtcttt ttgcttgtaa ccgttttcct tcatcaaata ttttaaaact tcgtgcggcg
     1501 gaatatcggg catcggataa tgctcgtctt cgtattgctc aatcaaaacc gctaataatt
     1561 caagcagttt ttcttcttca ggactaagct catcttcctt aattcccttt gtcatcaagc
     1621 ggtcaacttc tttggtcagt ctttcgagtt cttcgtcgct cgtaatgacc gaaggcaaag
     1681 tctgcattaa aagcttcccg tatttttgtt cgtcaacatt cgcagtcatc ttcgtaaatt
     1741 ctcttgtcgt attcggcgtg tgtcagaaca aatctgatta acactttttt gctcggataa
     1801 tatattttcg taattaaccg atatttattt ccgccgatat taaaaatcgt acaaacgccc
     1861 gctaaatcgg cgtgcggaaa atcttttctg acatcagcta agtttttcca atcagctttt
     1921 ttggtaactt tataccaatt caaaagcgcc gtttcggcat ctggatgcgt ttcccaaaac
     1981 tcccgcagag ttcttttggt aattactcgc attaaattga tttcctttat tcattgccga
     2041 gtcaaaactc tgtcttgggg ctaatctttt aattccgcat aaattctctc aaaaagagaa
     2101 aggaaatact gctatgctct cattttgaga aatcctcgtc aataaaaatt tctttctttg
     2161 acctaaaaca gcggaggatt taccattaaa ttttcgctca aaatttatgg atattctttc
     2221 gtcactaaat ccgcagcagc aggaagccgt gcagacgacc gaaggcgctc ttttgattct
     2281 ggcgggcgcg ggttcgggca aaacgcgcgt cattacggtt cgcatcgctt atttaatcgc
     2341 cgaaaagggc gtcgcgccgt ataacattct cgccgtgacg tttacgaaca aagccgccgg
     2401 tgagatgcgc gagcgcgtcg aatctttgct caagggacgc gggctgcaat cggcgccgct
     2461 gatttccact tttcacagtc tttgcgtgcg catcttaagg caggacatcg agcatctttc
     2521 ggaaggctat tcgaaatcct tcacgattta cgacacggac gattcgatga aagtcatcaa
     2581 ggcttgcgtc aaagacatcg gcttggacga caaacagctt ccggctcggc aggtgcaatc
     2641 cgccatcagc gcctcgaaaa atcgcggcga ggattttgaa gattacgcct cgcgcatcga
     2701 atacacggac gaacgacggg cggcgattgc cagagttttt aaaatgtacg aagagcgcct
     2761 gaacaattcg aacgcgctgg atttcgacga tttattaata aaaaccgtgc ggcttttgcg
//
LOCUS       DASWNE010000394         5734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_48601, whole genome shotgun sequence.
ACCESSION   DASWNE010000394 DASWNE010000000
VERSION     DASWNE010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5734
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48601"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="VGB68_07935"
     CDS             complement(<1..673)
                     /locus_tag="VGB68_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049198.1"
                     /translation="MSKKTLPEENPSNKLSKPQSKPIRVVSIDEVAQRLYVGSDYNKA
                     LVQKHSRFALFLGAGASADSNIKLANQMIDDFKDKIFKIKCADDRLKEKVENITRTVL
                     DPQKRRQQFWETTKDWFGEQDWYRDDDSEYSKCFEACYPKEVDRRNYIEITVGAAKPS
                     FGYVVLANLIKKEIIKTIITTNFDDLVYFACTSYTSTRPVVYAHGGFASEVSFSSARP
                     KILKLH"
     gene            complement(936..1649)
                     /locus_tag="VGB68_07940"
     CDS             complement(936..1649)
                     /locus_tag="VGB68_07940"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY0049199.1"
                     /translation="MITTIAFDADDTLWHNERLFISIKDKYKSLLSEYHDGDWIEERL
                     DEAETRNIQHFGYGIKGFTLSMIETACELTEGRIRGDKILEIIDFAREMLAAPIDVLE
                     GVRETIEALSGEYLLMVITKGDLLDQEAKLARSGLGDYFKAFEIVPRKDRGIYKYILR
                     RHQIRAEEFVMVGNSLKSDILPVLEIGAAAVHVPYETEWFHEKVSDDELKDKDFARLN
                     SIAELPAWLKKNGKRQTKR"
     gene            1888..2451
                     /locus_tag="VGB68_07945"
     CDS             1888..2451
                     /locus_tag="VGB68_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049200.1"
                     /translation="MEPLNQEVVTKVLNSPAALSIKTFFVGGNMITGSFYQALAAQMS
                     MFECLNVLHYPNLKGASAMYDSGSNALLLRFKQPGSATETSLIIHETTHAICDMLQMN
                     ISVMESEMMAYIAQCQFYRGLRQPSRLWSQDAKKDAVFEFATNIAEALQAGTTPSSQD
                     FESLRQAVTKHPYYKNSYGKSANYDGI"
     gene            complement(2577..5462)
                     /locus_tag="VGB68_07950"
     CDS             complement(2577..5462)
                     /locus_tag="VGB68_07950"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF009520.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEY0049201.1"
                     /translation="MTHNLFNTRQTFKTGGGEDGVFYSLPQLETEGVGAVSRLPVSIR
                     IVLESVLRNFDGKKVQEKDVRNLASWGASAERAEEIPFTVARVLLQDFTGVPLLVDLA
                     AMRSAVERMNRDTKLIEPLVPVDLVIDHSVQVDYSGSEAAYQRNMEIEFNRNEQRYRF
                     LKWGTQAFNGFAVVPPGIGIVHQVNLEYLAKGVFEREGVYFPDTLVGTDSHTTMINGL
                     GIVAWGVGGIEAEAGMLGQPVYFLTPDVVGVNLSGELPQGATATDLVLRITEMLRKAK
                     VVGKFVEFFGEGASSLHATDRATIANMAPEYGATMGFFGVDDKTLEYFRATGRDEAHI
                     ETIRNYYEAQKMFGIPQEGEVDYTTVLNLDLTTITPSVAGPKRPQDRIELSNLDDRFK
                     ELFTHSVAEGGYGKNWEDLEKRYMVTMGSHTTKVVAGGGSQNPLSVPLPILNSPVQGD
                     LISRKNTNVSTEVEMMNNRPTPDRVEFAPEDCPPQQTTIGHGDVLIAAITSCTNTSNP
                     SVMIAAGIVAKKASEKGMKVPPYVKTSLAPGSRVVTEYLEKTGLQKYLDEIGFNLVGY
                     GCTTCIGNSGPLDAGIEEEIVENDLVAASVLSGNRNFEARVHQNIKANFLMSPPLVVA
                     YALAGTVVRDVYLEPLGKDKDGNDVFLKDIWASLDEVKELLQAAFEPETYKRLYSEFA
                     EQNPLWNSIPSTTGAVYEWDRDSTYIQEPPYFEDFSMNAGKFANIEGARAMAIFGDSV
                     TTDHISPAGSIKPNSPAGLYLQEKGVEPQNFNSYGSRRGNHQVMVRGTFANVRIKNLM
                     VAPIEGGFTKHQPDGEEMSIYDAAMRYMEAGTPLMVFAGQEYGTGSSRDWAAKGTRLL
                     GVKAVVAQSFERIHRSNLVGMGVLPLQFKEGESAQSLGLDGTETFDLTGLDGAIKPRQ
                     EVTLRITKQTGERQEATLILRIDTPIEVEYFQHGGILPFVLRQLING"
BASE COUNT         1333 a         1501 c         1437 g         1463 t
ORIGIN      
        1 cgtggagctt gagaatcttc gggcgcgccg aagagaaaga gacttccgag gcgaaaccgc
       61 cgtgcgcgta gacgaccggg cgcgtcgaag tgtagctggt gcaggcgaaa taaaccaaat
      121 catcgaaatt ggtggtgata atcgttttga taatttcttt tttaatcaaa tttgccagca
      181 cgacgtagcc gaacgagggt tttgccgcgc cgaccgtgat ttcgatatag tttcggcggt
      241 cgacttcttt cgggtaacag gcttcgaagc atttgctgta ttcgctgtcg tcgtccctgt
      301 accaatcctg ctcgccgaac cagtctttcg tcgtttccca aaattgttga cgccttttct
      361 gcgggtctaa aaccgtgcgc gtgatgtttt cgactttctc ttttaagcgg tcgtcggcgc
      421 atttgatttt aaagattttg tctttaaagt cgtcaatcat ctgattcgcc agtttgatat
      481 tggaatcggc tgaagcgccc gcgccgagaa acagtgcaaa ccgcgaatgt ttttgcacga
      541 gcgccttgtt atagtcggag ccgacgtaga gtctttgagc cacttcgtcg atgctgacga
      601 ctcgaatcgg tttcgattga ggtttggaaa gtttgtttga aggattttct tcgggtaaag
      661 tttttttcga catattgaaa acctctcgga agctgtttcg gttaaagcgc gtcaagtgaa
      721 atcgagccgc tcgaaaaacg cgctcccagt ataaaaggtc aaagagaaat tgcaaaaaat
      781 caagaaagaa ttatttaacg taaaaaagat aaaagaaaaa gccggactgt gcaaacagcc
      841 cggctctttc ggaatttttt gaagataagg cggaaacaag agagctcgcg gcggtcgctg
      901 cgctcggatt agccgtcgaa gggctgactt gccgattatc gtttcgtttg ccgcttcccg
      961 ttttttttca gccaggcggg cagttcggcg atgctgttta atcgcgcaaa gtccttgtct
     1021 tttaattcat cgtcggagac cttttcgtga aaccactcgg tttcatacgg gacgtggacg
     1081 gcggcggcgc cgatttccag aacgggcagg atgtcggatt tcagagaatt tccgaccatc
     1141 acgaattctt ccgcgcggat ttgatgccgg cggagaatgt atttataaat cccgcggtct
     1201 tttcgcggaa cgatttcaaa cgccttgaaa taatcgccca agccgctgcg cgccagcttt
     1261 gcttcctggt cgagaaggtc gcccttggtg ataaccatca ggagatactc gcccgataag
     1321 gcttcgattg tttcccggac gccttcgagc acgtcaatcg gcgcggcgag catctcgcgg
     1381 gcgaaatcga ttatctccag aatcttgtcg ccgcggatgc gtccttcggt cagctcgcag
     1441 gcggtttcaa tcatcgagag cgtaaagcct ttgatgccgt agccgaaatg ctggatgttg
     1501 cgcgtctcgg cttcgtccag acgctcttct atccagtcgc cgtcgtggta ttcggacaaa
     1561 aggcttttgt atttgtcctt gatggagata aaaagacgct cgttgtgcca gagcgtatcg
     1621 tcggcgtcga aggcaatggt cgttatcatt aaattattcg tttcatccaa ttttattgac
     1681 agtttatcaa agccgaaacc aataaaaaaa ttaaagcacc ggatatgatg attattctca
     1741 cgcggaaaag ccggaagcgt tttttttccg aaagtttttt tcaatcgatg atagttttcc
     1801 gcccgaatct ccgcatttag aaatgagcgg cgaagatttt tttatccgga cgcgttttca
     1861 attttacttt taataaggcg gaatagaatg gaaccattga atcaggaagt tgttacaaaa
     1921 gttttaaata gcccggcagc cctgagcatc aaaacttttt ttgtcggcgg caatatgatt
     1981 accggctcgt tttatcaggc attagcggcg caaatgagta tgttcgaatg cctgaacgtt
     2041 cttcattacc cgaatttaaa aggcgcgtcg gcaatgtatg attcgggttc caacgctctg
     2101 cttttgagat tcaagcaacc gggcagcgct accgaaacgt cgctgattat tcacgaaacg
     2161 acgcacgcca tctgcgatat gctgcaaatg aatatctccg tgatggaatc ggaaatgatg
     2221 gcttatatcg cccagtgcca gttttacaga gggcttagac aacccagtcg cctgtggtcg
     2281 caggacgcca agaaagatgc cgtatttgaa tttgcgacga acatcgccga agcgcttcaa
     2341 gccggcacga cgccttcgtc gcaggatttc gaatcgctgc gtcaggcggt caccaaacat
     2401 ccttactaca aaaacagtta cggcaaatca gccaattacg acggaattta aacgaataag
     2461 ttcggaatcg gtaatacgca gagttaaatt gaaaaggacg cccgcgaaag gcgtcctttt
     2521 caatttaact ctggaattcg ggcgctgaag gttcgacgcc cggaactcta ttttctttag
     2581 ccgttaatca actggcgcaa aacaaacggc aaaattccgc cgtgctggaa atattcgact
     2641 tcaatcggcg tgtcgatgcg cagtattaaa gtcgcttcct gcctctcgcc cgtctgcttc
     2701 gtgattctca gcgtcacttc ctggcgcggt ttgattgcgc cgtcgagccc cgttaaatcg
     2761 aacgtttccg tgccgtcgag tcccagagac tgcgcgcttt cgccttcttt aaactgcaag
     2821 ggcagaacgc ccatcccgac cagattcgag cgatgtatgc gctcgaacga ttgcgcgacg
     2881 acggctttca cgcccagcag gcgcgtgcct ttcgccgccc agtcgcgcga cgaacccgtg
     2941 ccgtattcct gcccggcgaa aaccattaaa ggcgtgcccg cttccatata tctcatcgcc
     3001 gcgtcgtaga tactcatttc ttcgccgtcg ggctgatgtt tcgtaaaacc gccttcgatg
     3061 ggcgcgacca tcagattttt gatgcgcacg ttggcaaacg tgccgcgcac cataacctgg
     3121 tgatttccgc gccgcgagcc gtaggagtta aagttctgcg gctcgacgcc cttttcctgc
     3181 aaataaagtc cggcgggcga attcggtttt atcgaaccgg cgggcgagat gtggtcggtc
     3241 gttaccgaat cgccgaaaat cgccatcgcg cgcgcgcctt cgatgttggc gaatttgccc
     3301 gcattcattg aaaaatcttc aaaatacggc ggctcttgaa tataagtcga gtctctgtcc
     3361 cattcgtaaa ccgcgccggt cgtcgaggga atcgaattcc acaaaggatt ctgctcggca
     3421 aattcgctgt aaagacgttt ataagtttcc ggctcgaagg cggcttgcag gagttccttg
     3481 acttcgtcga gcgatgccca gatgtctttc agaaaaacgt cgttgccgtc tttatctttt
     3541 ccgagcggtt cgagatagac gtctctgacg acggttcccg ccagcgcgta agcgacgacc
     3601 agcggcggcg acatcagaaa attcgccttg atgttttgat gaacgcgcgc ttcgaagttg
     3661 cggttgcccg acaagacaga cgcggcgacc aaatcgtttt cgacgatttc ctcttctatc
     3721 ccggcgtcga gcggaccgga attgccgatg caggtcgtac agccgtaacc gacgaggtta
     3781 aaaccgattt cgtccagata tttctgcaag ccggtttttt ccaggtattc cgtgacgacg
     3841 cgcgagccgg gcgcgagcga ggtttttaca tacggcggaa ctttcattcc cttttccgaa
     3901 gcttttttcg ccacgattcc ggcggcaatc atcaccgacg ggttcgacgt gttcgtgcac
     3961 gaagtaatcg cggcaatcaa aacgtcgccg tgaccgatgg tcgtctgctg cggcgggcaa
     4021 tcttccggcg cgaattcgac gcggtcgggc gtcgggcgat tgttcatcat ctcgacttcg
     4081 gtcgaaacgt tcgtattctt ccggctgatt aaatcgccct gaaccggcga atttaaaatc
     4141 ggcagcggaa ctgaaagcgg attctgcgag ccgccgcccg cgacgacttt tgtcgtgtgc
     4201 gagcccatcg tcaccatata gcgtttttcg aggtcttccc aatttttccc gtaaccgccc
     4261 tcggcgaccg agtgcgtgaa aagctctttg aatcggtcgt ccagattgga tagctcgatg
     4321 cggtcctgcg gtcgtttcgg tccggcaacc gaaggcgtaa tcgtcgtcaa atcaagattc
     4381 agaaccgtcg tgtaatcgac ttcgccttcc tgcggaatgc cgaacatctt ttgcgcctcg
     4441 taataattcc tgatcgtttc gatgtgcgct tcatcgcgcc ccgtagcgcg gaaatattcg
     4501 agcgttttat cgtcaacgcc gaaaaatccc atcgtcgcgc cgtattccgg agccatattc
     4561 gcaatcgtcg cgcggtcggt cgcgtgcaag gaactagcgc cctcgccgaa aaattcgacg
     4621 aatttgccga cgactttcgc tttgcggagc atctccgtaa ttctcaaaac caaatccgtc
     4681 gccgtcgcgc cctgcggcaa ttcgcccgaa agattgacgc cgacgacatc cggcgtcagg
     4741 aaatacacgg gctgtccgag cattccggct tcggcttcga ttccgccgac gccccacgcc
     4801 acgatgccga gaccgttaat catcgtcgtg tgcgaatcgg ttccgaccag agtatcggga
     4861 aaataaacgc cttcgcgctc gaaaacgcct ttggcgagat attccagatt gacctggtga
     4921 acgatgccga tgcccggcgg cacgacggcg aagccgttaa acgcttgagt tccccatttc
     4981 aaaaagcgat aacgctgctc gttgcggtta aactcgattt ccatattgcg ctgataagcc
     5041 gcttcgctgc ccgagtaatc gacctgcacg gaatggtcaa tcaccaaatc gaccggcacg
     5101 agcggctcaa tcagtttcgt gtcgcggttc atacgctcga cggcggaacg catcgccgcc
     5161 aaatcgacca gcagcggaac gcccgtaaaa tcctgcaaaa gaacgcgcgc caccgtgaac
     5221 ggaatttctt cggctcgctc ggctgatgcg ccccagctcg ccagattgcg cacgtctttt
     5281 tcctgcactt ttttcccgtc gaagtttcgc aaaaccgatt cgagcacgat gcgaatcgaa
     5341 accggcagac gcgaaaccgc gccgacgcct tccgtctcca gctgcggcag gctgtaaaaa
     5401 acgccgtcct caccgccgcc cgttttaaat gtttgtctgg tattaaaaag attgtgtgtc
     5461 atatattttt aattgcggca gatagattta aaattgccgt gtaaatcaaa ctcatattgt
     5521 aaaacggaaa tcgaaaaact caagcaattc cgccttttag taaaaaaatt catctaggcg
     5581 tccgcgcaga ttttcgattc taatatcttt gcgcgttaaa tattaatatc taagcttcga
     5641 attacaattt ccgcgccgaa atttatattt ttaagaattt aacttctcaa cttttcagtt
     5701 tcgcctaatc gcgctatatt caagtttcga taat
//
LOCUS       DASWNE010000395         6839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_506426, whole genome shotgun sequence.
ACCESSION   DASWNE010000395 DASWNE010000000
VERSION     DASWNE010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6839
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506426"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1123
                     /locus_tag="VGB68_07955"
     CDS             <1..1123
                     /locus_tag="VGB68_07955"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HEY0049202.1"
                     /translation="MPVRSYAMMHAAMFDAVNSIDGTYTTYLIDVPSTKNASITAAAA
                     QAARDVLTGLYPSRQAIFDAELADSLEGIPQNRALQGIRVGQAVAVRMLNARANDGWH
                     APSPTYVLPATPGNWQPTPPANSAATFTHYPNVVPFAVASGSQFSPAPPPALTSAEYA
                     AALNEVREIGAVNSATRTDDQTKVAQLWANVNTPTNFLFVWNNVARTLALSRATTTIE
                     NARLFALTNIALHDALQTSFAGKFQYGLWRPVTAIRRADEDGNAETTQDAGWSSLIAT
                     PPYPSYAGNMATIGASQSTILALFFGRDDISFQHTWEGAGGATRSYVGFTAMANEQAE
                     ARIYGGIHFRFDNVAGQSIGRNVANYVFQNLMRPRACVR"
     gene            1147..1953
                     /locus_tag="VGB68_07960"
     CDS             1147..1953
                     /locus_tag="VGB68_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049203.1"
                     /translation="MKKSSILMTAFLIVFALGVQAASAQITITLPKFPKIKKPKPEQS
                     ETGSTNGNNQSNRTSSNGDGQTKESKTTALTGKCAGNIWVESHLEDIAKRQKEVDGFT
                     PDRGWLAGGSNYDHLLYAVSPTAREKWLAGANALDYKNCLASAFDRLAASAAIKLPLF
                     LPNTKAYAQHNPAEERLMKSKINDLADHKVFYIGIQEPSWLIDKNEFGLPKSRYKHGM
                     VWVRYTPNDHPYCRVYYINVIQDYAGGGTYGASYAAFIEDQLFGCPSGAK"
     gene            2178..2741
                     /locus_tag="VGB68_07965"
     CDS             2178..2741
                     /locus_tag="VGB68_07965"
                     /inference="COORDINATES: protein motif:HMM:NF023363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3455 domain-containing protein"
                     /protein_id="HEY0049204.1"
                     /translation="MKITNLKNIIGLVALFGVAVLALASSRVVRADDDMRTPELPASC
                     SSIQVEAGNKVSYHVYAIGVQVYKWNGASWTFKEPVANLYANGNYKGRGAGIHYAGPT
                     WESNSGSKVIAARVSNTGCTPEATAIPWLLLKTVSTDGPGIFGKVTYIQRVNTTGGLA
                     PATAGATVGEEKSVPYTAEYYFYRAEY"
     gene            2851..3684
                     /locus_tag="VGB68_07970"
     CDS             2851..3684
                     /locus_tag="VGB68_07970"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0049205.1"
                     /translation="MNAATLRMTSEMETLKTKLKATWISGDFGKIAGSFTAGAARFVE
                     RLNLKPGMRVLDVACGSGNQSIPAARAGAEVTGVDIAPNLIEQAGEWAANENLKIRFD
                     EGDAENLPYPDAEFDVVMTMFGAMFAPRPARVAAELLRVCRPGSVIAMANWTPTGFTG
                     QMFKIVGSHVKPPQNMPSPLLWGDEATVRERLGEGVSEINFNPRLISLTFPFGVKETI
                     DFWREFYGPTHKAFAALDEKGQAALHRDLEKLWADNNLSANGITHVKAEYTEVTAIRN
                     K"
     gene            complement(3784..4758)
                     /locus_tag="VGB68_07975"
     CDS             complement(3784..4758)
                     /locus_tag="VGB68_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049206.1"
                     /translation="MNLTKTLRLLLVFLSISGICSAQEFTEEENWIREIAGEYCRGYR
                     FLAYRFETFTKQDVIKAKEKLKSIKQFAPESEWEGVYFGNVALGDNGLLWSAEGGFLK
                     FYCYHELGRLDYGKVSESPGFVEIISEKPAASTPDQKPNGKTENKLIKVKFGESHYLV
                     PENSLQHFTTRAAGLGTSSDNYADFYWLKREDFEKKVFGLPVLPKEYKHLLRFPVETR
                     IISVGKRQIIRNKQSTEEYNFDDIHYPVTLDAGKNRNIKPGMNFYVDDLGEWVEITGV
                     FQKSSIGRIRRDFDENKNEQCRNGELGGGDIISCKKIKFGMKAKTKFN"
     gene            4935..>6839
                     /locus_tag="VGB68_07980"
     CDS             4935..>6839
                     /locus_tag="VGB68_07980"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0049207.1"
                     /translation="MTDKNNQAIKDGEEAEQNPINRLPFNETASSWLTAIIDSAEDAI
                     ISKTLEGIITSWNKSAQRIFGYTAEEIVGKSVLTLIPPDLQHEENIIIGKIKSGERLE
                     HYETTRVRKDGRLIDISLTVSPIKDLNGQIIGASKIARDISVRKQIEAKLKEQSEILE
                     TINHVGQMLSAELDQQKLVQAITDAATELTGAEFGAFFYNVYQADGGSYMLYALSGVP
                     REAFAGYPMPRATDMFGPTFRGEGTIRIDDVRQDPRFGNNSPYYGMPEGHLPVTSYLA
                     VSVTSRSDEVIGGLFFGHSKAGVFTETHERIIEGLAAQTATAMDNARLYDQAQKAIKD
                     REESLTREQEARRQAEIASQSKDEFLGLLSHELRTPLNAILGWTRMLTSTRMDEATVG
                     KAIKTIDRNAQLQARLIEDMLDVSRIMSGKLRLDTQPIDLTSIVHAAIDTLRPAAEAK
                     GIRVYVVLDYGAGMVLGDTVRLQQIAWNLLSNAIKFTPKQGSIKVFLERINSHLELTV
                     SDTGPGIDEDFLPFVFDRFRQADSSTVKKYGGLGLGLSIVKQLVELHGGTVEADNRKD
                     ESGAIFTVKLPVMVVRQQMDLRDNNSGRVHPVAGSGVVNFDCPPEIKGIRILVVDDDA
                     DARHLLMAILE"
BASE COUNT         1721 a         1721 c         1798 g         1599 t
ORIGIN      
        1 tatgccggtg cgcagctacg cgatgatgca cgcggcgatg ttcgacgcgg tcaattcgat
       61 tgacggcact tatacgactt atttaataga tgttccaagc accaaaaacg cgtcgataac
      121 cgccgccgcc gcgcaagccg cgcgcgacgt tctgacgggt ttgtatccga gccgtcaggc
      181 gattttcgac gccgagttag ccgattcgct cgaaggaatt ccgcagaacc gcgcattgca
      241 gggcattcga gtcggtcagg cggtcgcggt gcgaatgtta aacgcgcgtg ccaacgacgg
      301 ctggcacgcg ccgtcgccga cgtatgttct gccggcgacg ccgggcaact ggcagccgac
      361 gcctccggct aattcggcgg cgacttttac gcactacccg aacgtcgtcc cgtttgccgt
      421 cgcgagcggc tctcagttca gtcccgcgcc gccgcccgct ttaacgagcg ccgaatacgc
      481 cgccgcgctg aacgaagtca gggaaatcgg cgcggtaaac agcgcgacgc gcactgacga
      541 ccagaccaaa gtggcgcagc tctgggctaa cgtgaacacg ccgacgaatt ttcttttcgt
      601 ctggaataac gttgcccgta ccctcgctct gtcccgcgcg acgacgacca tcgaaaacgc
      661 ccgccttttc gcgctgacga atatcgcgct gcacgacgct ttgcaaacct cgttcgccgg
      721 caaatttcaa tacggcttat ggcgacctgt cacggcgatt cgccgcgccg acgaagacgg
      781 aaacgccgag acgacgcagg acgccggctg gtcgtcgctg atcgccacgc cgccgtatcc
      841 gtcctacgcg ggcaatatgg cgaccatcgg cgcttcgcaa tcgacgattc tcgcgctgtt
      901 tttcgggcgc gacgacatct cgtttcagca tacgtgggaa ggcgcgggcg gcgcgacgcg
      961 ctcgtatgtc gggtttacgg cgatggcgaa cgaacaggcg gaagctcgta tttacggcgg
     1021 aattcatttc cgtttcgata atgtcgccgg tcagtcaatc gggcgcaacg tcgccaatta
     1081 cgtttttcag aatctgatgc gtccgcgcgc ctgcgttcgc tagaaattat gaataggaga
     1141 aaaattatga aaaagagttc aattttaatg acggcgtttt tgattgtatt cgcgcttggc
     1201 gtgcaggctg cttcggcgca gattacgata acgcttccca aatttccgaa aatcaaaaag
     1261 ccgaaaccgg aacagtccga gactggttcg acgaacggca ataatcaatc gaaccggact
     1321 tcctcgaacg gcgacggtca aacaaaggaa agcaaaacga cagccctgac cggcaaatgc
     1381 gcgggaaaca tttgggtcga atcgcatttg gaagacatag ccaaaagaca aaaagaggtt
     1441 gacggcttta cgcccgaccg cggctggctg gcgggcggca gtaactacga ccatttattg
     1501 tatgcagtct cgccgacggc gcgggaaaaa tggctggcag gcgctaacgc gctcgattac
     1561 aagaactgcc tcgcttcggc ttttgacagg ctggcggctt cggcggcgat taaattgccg
     1621 cttttcctgc cgaacactaa agcttacgcg cagcacaatc cggcggaaga aagattgatg
     1681 aaatcgaaaa tcaacgactt agccgaccac aaggtttttt atatcgggat tcaggagccg
     1741 agctggttga ttgataaaaa cgagttcggg cttccgaagt ccagatacaa gcacggaatg
     1801 gtctgggtgc gctacacgcc gaacgaccat ccttactgcc gcgtttatta catcaacgtc
     1861 attcaggatt acgccggagg cggaacttac ggcgcaagct acgccgcttt tatcgaggat
     1921 caactgttcg ggtgtccgtc cggcgcgaag taaatcactt tcgatgcgga gcggctcgac
     1981 atcgagtgat tgaaatcaaa atcgctcgct tgcggagttg ccctgcgtcg ccggtcaatc
     2041 aatcggttga acgctagtgg tcgcaacttg cgtttttcaa aatctgatgc gtccgcacgg
     2101 atgcatcagc cggaaattcg atcggttaat tccaatcgaa atttagaact aaaaatataa
     2161 atatgaggag aatttttatg aaaatcacga acttaaaaaa catcatcggg cttgtcgcat
     2221 tgttcggcgt cgctgtgctt gcgctcgcct ccagccgggt cgtccgcgcg gacgacgata
     2281 tgcgcacgcc ggaattgccg gcttcgtgca gcagcataca ggtagaggcg ggcaataaag
     2341 tgtcctacca cgtctacgcg attggcgtgc aggtttataa atggaacggc gcgagctgga
     2401 catttaaaga acctgtcgcg aatttatacg ccaacggcaa ttataaaggc agaggagcag
     2461 gcattcacta cgccggtccg acctgggaga gcaacagcgg cagcaaggta atcgccgcgc
     2521 gcgtctccaa caccggctgc acgccggagg cgaccgcgat tccctggctg ctgcttaaaa
     2581 cggtttcgac cgacggaccg ggcattttcg gcaaagtgac ttacatccag cgcgtgaaca
     2641 caacgggcgg cttagcgccc gcgactgccg gcgcaaccgt cggcgaggaa aagagcgtgc
     2701 cctacacggc ggaatattac ttttaccgcg cggagtattg ataattgttc cgggtattcg
     2761 ggtaaacaca ttcttctccc tgcccgaata ccgaacatat ggagataaac cgaggaatta
     2821 agatgatttc aaataaaaaa aggaaaaaat atgaatgcag caacattacg gatgacatcg
     2881 gaaatggaaa cactaaaaac aaaattaaaa gccacctgga tttcgggcga cttcggtaaa
     2941 atcgccgggt ctttcaccgc cggcgcagcc agatttgtcg aacggttaaa tttaaaaccc
     3001 ggaatgcggg ttttagacgt ggcttgcggc agcggcaatc aatcgattcc ggcggcgcgt
     3061 gcgggagcgg aagttacggg cgtagatatt gcgccgaact taatcgaaca agccggagaa
     3121 tgggcggcaa acgaaaattt gaaaattcga ttcgatgaag gagatgcgga aaatctgcct
     3181 tacccggacg ccgagtttga tgtcgtgatg acgatgttcg gcgcgatgtt cgcgccgcgc
     3241 cccgcacgcg tcgcggcgga actgcttcgc gtttgtcgtc ccggcagcgt gattgcgatg
     3301 gcaaattgga ctccgacggg tttcaccggg cagatgttca agattgtcgg ctcgcacgtg
     3361 aagccgccgc agaatatgcc ctcgcctctg ctttggggcg atgaagcgac ggtgcgcgag
     3421 cgtctgggcg aaggcgtctc ggaaattaat tttaacccgc gccttatttc attgacattc
     3481 ccgttcggcg tgaaggagac gatagatttc tggcgcgagt tttacggtcc gacgcacaaa
     3541 gcctttgccg cgctcgacga gaaagggcag gcggctctgc atcgggattt ggaaaaattg
     3601 tgggctgaca acaatctctc cgcaaacggc ataacgcacg ttaaagcgga atatacggag
     3661 gtaacggcaa ttcgcaataa ataagctttg ttgatttttt attcattctg cgggcgagag
     3721 cgaattcgct ctcgcccgca gaatgcagga aaatacgggc gaataccaaa catttcgcag
     3781 actttaatta aatttagttt tagctttcat tccgaatttg atttttttgc aggatataat
     3841 atcgccgccg ccaagctcgc cgttccgaca ttgctcgttt ttgttttcgt cgaagtctct
     3901 cctgattctt ccaatcgagc ttttctgaaa taccccggtt atttcaaccc attcgcccaa
     3961 atcatcgaca tagaaattca tacccggttt tatgttcctg tttttacccg catcgagagt
     4021 tacgggatag tgaatatcgt caaaattata ttcttcggtg ctttgtttat tccggataat
     4081 ttgtcttttg ccgacactga ttattcttgt ttctaccgga aaacgaagca aatgcttata
     4141 ttctttgggg agtacgggaa gaccaaaaac tttcttttca aaatcttcgc gtttcaacca
     4201 gtaaaaatcg gcgtaattat ccgagcttgt gcctaagccg gcggctctcg tcgtaaaatg
     4261 ctgcaggcta ttttcaggaa ccagataatg ggattcaccg aacttgactt tgattagttt
     4321 gttttcggtt ttaccgttcg gtttttggtc gggagtcgat gcggcaggct tttccgagat
     4381 tatctcgacg aaacccggag actcgctaac tttgccgtaa tccaatctgc cgagctcgtg
     4441 atagcagtaa aatttgagaa atccgccttc cgcactccaa agcaatccat tgtcgccgag
     4501 cgcaacattt ccgaaataaa ctccttccca ttcgctttcc ggcgcgaatt gttttataga
     4561 ttttagtttt tctttagcct taattacatc ttgttttgtg aaggtttcaa agcggtaggc
     4621 aagaaaccga tacccgcggc aatattctcc ggcaatttct cttatccaat tttcttcttc
     4681 cgtaaattcc tgcgcggaac atatcccgga aattgataga aaaactaaaa ggagtcgaag
     4741 agtttttgtt aaattcattt tcttcgatgg caggctctga aaagtgtctt ttacaggaca
     4801 catcttactc tgttttaatc ggctcgaaaa ggcgcgttta ttaaacaatt taaatatagc
     4861 gtcattatcg tctatttgtt aaactgattt tagtcagtat gcttttctaa actgctttta
     4921 tcaatcaagg ggttatgacg gacaaaaaca atcaagcgat caaagacggg gaagaagccg
     4981 aacaaaatcc aattaaccgt ttaccattta atgaaaccgc ctccagttgg ctgacggcaa
     5041 tcatcgattc ggctgaggac gcgattataa gcaaaacgct cgaaggcata atcaccagct
     5101 ggaacaaaag cgcccagcga atcttcggtt atacagccga agaaatcgtc ggaaaatcgg
     5161 ttttaactct gattccgccg gatttgcagc acgaggaaaa tataattatc ggtaaaatca
     5221 aaagcggcga gcggctcgaa cactacgaga cgaccagggt cagaaaagac gggcgtctga
     5281 tcgacatatc tctcacggtt tctccaatca aagaccttaa cgggcagatt atcggcgctt
     5341 ccaaaatcgc gcgcgacatc agcgttcgaa agcagataga agcaaaatta aaggaacaat
     5401 ccgaaattct ggaaacgata aatcacgtcg ggcagatgct ttccgccgag ctcgatcagc
     5461 agaagctggt acaagccata acggacgcgg ctacggaatt gacgggcgcc gagttcgggg
     5521 cttttttcta taacgtttac caggcggacg gcggctctta tatgctctac gccctgtcgg
     5581 gcgtgccccg cgaagccttt gccggttatc ctatgccgcg ggcgacggat atgttcggtc
     5641 cgacttttcg cggcgaggga accatccgca tagacgacgt gcggcaagac cctcgtttcg
     5701 gaaacaattc tccttattac ggcatgcctg agggacatct gcctgtgacc agttatctag
     5761 ccgtttcggt gacttcacgc tcggatgagg taatcggcgg actcttcttc ggtcattcga
     5821 aagcgggagt ctttacggaa actcatgaac gtattattga agggctcgcc gcccagactg
     5881 caactgcgat ggataatgcg aggctctatg accaggctca aaaggctata aaagatagag
     5941 aagaatcttt aactcgcgaa caggaagcgc gccggcaagc ggagattgcc agccagagca
     6001 aggacgaatt tctggggctt ctttcccatg agcttagaac cccgctcaat gctattttgg
     6061 gttggacacg aatgttgacc tcgacccgga tggacgaggc aactgtcggc aaagccatta
     6121 aaacaataga ccggaacgcg cagcttcagg caagactgat tgaagatatg ctggacgttt
     6181 cgcgaataat gtccgggaaa ctccggctcg atacgcagcc gattgacttg acaagcatcg
     6241 ttcatgccgc gatagatact ctgcgtccgg cagccgaagc caagggaata cgtgtttacg
     6301 tagtgctcga ttacggggcg gggatggttt taggcgacac ggttcgtctt cagcagattg
     6361 cgtggaacct gctttcaaac gccatcaaat tcacgcccaa gcaaggaagc atcaaagtat
     6421 ttctcgaacg aatcaattcg catcttgaac ttaccgtcag cgataccgga ccgggcattg
     6481 acgaagactt tctgccgttt gtgttcgatc gtttccggca ggcggacagc tcgaccgtta
     6541 aaaaatacgg cggtctcgga ctaggacttt caatcgtcaa acaacttgtc gagcttcacg
     6601 gcgggacggt ggaagccgat aatcgcaaag acgagtccgg tgcgatattt actgttaagc
     6661 tgccggtaat ggtcgttcgt cagcaaatgg atttaagaga taacaattca ggaagggtgc
     6721 atccggttgc cggcagcggc gttgtgaatt ttgactgccc tccggaaatt aagggaatca
     6781 ggattctagt ggttgacgat gacgccgatg cgcggcattt gttgatggcg attctcgaa
//
LOCUS       DASWNE010000396         2654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_225140, whole genome shotgun sequence.
ACCESSION   DASWNE010000396 DASWNE010000000
VERSION     DASWNE010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225140"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1559)
                     /locus_tag="VGB68_07985"
     CDS             complement(<1..1559)
                     /locus_tag="VGB68_07985"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0049208.1"
                     /translation="MRFAGFVFVCLLLASLFFLPGVSSQEMRSENNSEQKKTEDSFVA
                     VRAKKRGFPRVNFADGRDLSFAGKAQQNAGSAKLLTSADFDSDGTADLMTADFGGSVK
                     LYRGNADSIYPNSVEAQARRANGNFSEEAFLESEKSFQIPVSPDFLAAGDFNADGHQD
                     ILAAQRNAGALYLLAGDGAGNFATPSQIKVDGAIAAFAVGEIGQRNGQTDVVIALSGK
                     GGNRLAVFEHPEGAFKNKPEIIKLPAAANNLAIGHLDTDYFGDIAVSGGNLLTIVRGR
                     GQIHPWDLMPEYDLKRPAPIVETRNLPFQIAALATGYFTEKRGESLAMLTTGGSIEIL
                     DSPRAENKRASLKPENLKNTGQRLPLKESSKRLSKFKTFAETPAPSDTLRKIKEINEP
                     ARPELTERQREALLAGNRNLSPESSRRAKENFLRAVSPGAAAPLARWKLETIASDSRL
                     AGAASSFSRSNLVKVRVSDSGRDELAFIDAGAKQIHLMIRENLKRSRAANANEIISLD
                     VESAPLAILPMR"
     gene            1741..1950
                     /locus_tag="VGB68_07990"
     CDS             1741..1950
                     /locus_tag="VGB68_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049209.1"
                     /translation="MSSGSNKQRRAGNREIGNYSNSTSFERTAAAKAPAPGSFMLEKG
                     NGNSSPGLFCLKLREMPAAAFPVCD"
BASE COUNT          635 a          682 c          664 g          673 t
ORIGIN      
        1 cgcatcggca gaatcgctaa aggagcgctt tcgacgtcga gcgaaattat ttcgttggca
       61 ttcgccgcgc ggctgcgttt cagattttcg cgaatcatca ggtgaatctg ttttgcgccc
      121 gcgtcgataa acgccagctc gtcgcgtccc gaatcggaaa cgcgaacttt gacgagattc
      181 gaccgcgaaa aggagcttgc cgcgccggcg agccgcgaat cggacgcgat ggtttccaat
      241 ttccatcgcg cgagcggagc cgccgcgccc ggcgaaaccg ctctgagaaa attttccttc
      301 gctcggcggc tggattcggg cgagagattc ctgttgccgg cgagcagagc ctcgcgttgc
      361 cgctcggtca attccggtcg tgccggttca tttatttcct tgattttgcg cagcgtatcg
      421 ctcggcgcgg gcgtttcggc gaacgttttg aattttgaaa gacgttttga actttctttc
      481 aaaggaagtc gctgcccggt atttttcaaa ttttccggtt tgagcgatgc gcgtttattt
      541 tccgcccgcg gtgaatcgag gatttcgatg cttccgccgg tcgtcagcat ggcgaggctc
      601 tcgcctcttt tttcggtaaa atagccggtc gccagagcgg cgatttgaaa aggcaggttt
      661 cgcgtttcga ctatcggcgc gggtcgtttc aaatcgtatt ccggcatcaa atcccatgga
      721 tggatttgtc cgcgaccgcg cacgatggtc agaagattgc ctccggaaac cgcaatatcg
      781 ccgaaataat ccgtgtccag atgtccgatt gccagattgt tcgccgccgc cggcaatttg
      841 atgatttcgg gtttgttttt aaaagcgcct tccggatgtt caaacacggc gagccggttg
      901 ccgcctttgc ccgagagggc aatcacaacg tcggtctgcc cgttgcgctg cccgatttct
      961 ccgaccgcaa aagcggcaat cgcgccgtcg accttgattt gagatggcgt ggcgaaattt
     1021 cccgcgccgt cgcccgcgag cagataaagc gcgcccgcgt ttcgttgggc tgccagaatg
     1081 tcctgatgcc cgtcggcgtt aaaatcgccc gccgcgagaa aatcgggcga aaccggaatt
     1141 tgaaaacttt tttcgctctc taaaaacgct tcttcgctga aatttccgtt tgccctgcgc
     1201 gcttgtgcct cgacgctgtt gggataaatc gaatcggcat tgccgcgata aagctttacg
     1261 cttccgccga aatcggcagt catcaaatcg gcggttccgt cggaatcgaa atccgccgaa
     1321 gtcagcagct ttgcgcttcc tgcgttttgc tgcgctttgc ccgcaaaact caaatccctg
     1381 ccgtccgcaa aattgacgcg cggaaacccg cgttttttag cgcgcaccgc gacaaaggaa
     1441 tcctcggttt ttttctgctc cgaattattt tcagagcgca tttcctgtga ggaaactccc
     1501 ggcagaaaaa acaaagacgc cagcaacagg cagacaaata caaaaccggc gaaacgcata
     1561 atcagacctc tttttatcga aaatttaaag acttcaaaaa tacaagcgaa aaacttatag
     1621 cttcttgtaa aggtataagc gaaaaatttc gccgcagtca gccaaatttt tttgaaaatt
     1681 ttttgtttcg gtaaaaaccg gagtggaatt ttttttgcaa aatccccgcg agcgcggatt
     1741 atgagcagcg gcagcaacaa gcagcggcgg gcaggaaaca gggaaatcgg aaattactcc
     1801 aattctacaa gctttgagcg gacggcggcg gcgaaagctc cggctccggg ttcttttatg
     1861 cttgaaaaag gcaacgggaa tagttcgccc ggattgtttt gtcttaagct cagagaaatg
     1921 ccagccgccg cctttccggt gtgtgattaa agcgcgtctg actatcgccg ttttcttttc
     1981 caaatcaata tcgtttccat taaagcgaaa gatattgatt taatcggcag cctgtcgcaa
     2041 agcaaaggaa aacatcgctg caaattcgtt tcccggcaaa gcgtctaaaa atatcatttc
     2101 ttcattttga cttaaaacat cctgagccgg tcaagctcgt tttgcctttg gttaaaaaaa
     2161 aatttgtccg accccggagc cggcggacaa atttatagaa taaaatttat ttctttcctg
     2221 attcggctcc gttctctcta aaatctcccg ggcttcgtag cagatttgct gaaaaaatgc
     2281 ggcgagctcc gcgtttataa ggcgcgcgtg ttcgtcgatt aacagcctgc gcccgtcaga
     2341 atcattttca attaatttcc gggcaagctg ttggtgattg atttttgaga aaaagagatt
     2401 ggctaaaaat aattataaat ttaaggttta tccgcaagct agtataaaac ggggtctgtt
     2461 tctaaacaaa tttaataaag gcggcgtgag aaatatattt gccgccggtt taaaagcaaa
     2521 aaggcggaaa tgcggcagct gcgaaccgcg catcccgaat gacgaaggcg cggacgataa
     2581 acgccgaatt cgatacgccg aatccgctat tccgccttca gtctttatca ctccgcatcc
     2641 cgccgacacg ctcc
//
LOCUS       DASWNE010000397         5510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_154330, whole genome shotgun sequence.
ACCESSION   DASWNE010000397 DASWNE010000000
VERSION     DASWNE010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5510
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154330"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..2067)
                     /gene="selB"
                     /locus_tag="VGB68_07995"
     CDS             complement(142..2067)
                     /gene="selB"
                     /locus_tag="VGB68_07995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HEY0049210.1"
                     /translation="MDIIVGTAGHIDHGKTSLVRALTGIDADRLPEEKRRGITIDLGF
                     AELDLGDVRIGFVDVPGHEKFVKNMLAGASGIDLVALVIAADEGVMPQTREHFDICRL
                     LETKAGLIVLTKTDMVDAELLELVRLDAQELVENSFLENAPVIAVSSKTGEGIDELKE
                     AFKNLAAQIPPPKNETVARLPIDRSFTVKGFGAVVTGTLIAGEINEGDELEVLPLKRK
                     VRVRGLQTHGKSVKTAHAGQRTAVNLGGIEHAEIERGMVLCEKDALQPTQIFDAEIEV
                     LKDAKRALKSRQRVRAHLGTIEALARIQVLNEAGEIAPGARDFVQLRLEAPAAAIPGE
                     RFIIRSYSPQVTIAGGRVLDAQAMKHRRKDTESARRVLQNLIEAENRNDKARQARIYL
                     ETAGESGARFADLQARTGWRESVLRKALAENSEKKSVVETGGFFVARTPFDNLKAKTL
                     AEIEAHHRREPLARGIGRETLREKIFARLPAEIFKAILAALEKDARIAAEKDLVRAVA
                     HNPELSAEEKILRERLEKIYAQAKLEVPALEAALDEAIRGTKLDRQQARKVFQLFLNA
                     AEIVKVTEDFYFRREALDRLVNQLKNYAAQTPDRLIDMATFKEIAGISRKYAIPLLEY
                     FDREKHTRRAGDKRLIL"
     gene            complement(2148..4154)
                     /locus_tag="VGB68_08000"
     CDS             complement(2148..4154)
                     /locus_tag="VGB68_08000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0049211.1"
                     /translation="MVKITTKLVQLDAVVTDEKGNQVTDLTAADFEVWQDGKPQKITN
                     FSYVNTVASSSAPVKIEKKDKNAVLPPPPAGRAPGGRLITFVVDNCTASGSGINAARE
                     ALNKFVREQMLAGDMVAIYQTTGGSSLLQQFTSDREQLLRLVRKIVWYPNRGCLTAGG
                     PVFAPVRDDVLTETEREIKEAAEDASRDIQSVSVINVLNYIVRGLGRVGGRKVVFLLS
                     DGIPLRTRSDKSLGTHSVRTEAALRGLTDAANRAAVVFNTIDTRGLLNTDAIEAVDEV
                     SSGSTDKVAAERRSLLLGMQDGLNFLARETGGTFSKNQNFLDVPIGRALNLEKGYYLL
                     AYAPDDETFKGKNFNQVSIKVSRPGLKVVSRAGFLGRVDEKQNVSRKTGDSELYEAVV
                     APLPRTGMNLRLTAFFGNTPAKGNFVRSLVHVDGKDITFADDANGSKKVALDVFAVTL
                     DEKNDVVDEFNHTHKVLIPAQAVELIRQNGLVYSVDVPVKKEGTYNFRLAVRDAGSKM
                     LGSASQIIQVPALKKDKLFLSGLTVSRVDADGKFIVPAEVKPENAIAAALNTSVPAIR
                     QFQRGMILAYSYTLYNAALDKAANQPKLTVQVNLYRDGQLVTQGQPQASQLEKQDDMT
                     RIADYGYMRLSPNMQPGDYALQIIVRDLLTNQTVSQWVDFQVTQ"
     gene            complement(4060..4674)
                     /locus_tag="VGB68_08005"
     CDS             complement(4060..4674)
                     /locus_tag="VGB68_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049212.1"
                     /translation="MIKGKREMSSDKRDLEADKHDLSEDKRRLSEDKHDLSKDKHDLS
                     KDKRYLSEDKRHLSLDKRHLSLDKRRLSAFKSRLSFRFAICVLNFAFQTRESNEIKQN
                     SLAARRRNRVRYVETAICPHRLGAAEKADLSFITFWSIRDEKVLFRFSYLFADDNFDF
                     RAANESYAAGRRRRGQNHDQARPARRSGDRRKRQSGDGFDRRRF"
     gene            4881..>5510
                     /locus_tag="VGB68_08010"
     CDS             4881..>5510
                     /locus_tag="VGB68_08010"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0049213.1"
                     /translation="MRTLRFMTALTLLFSVCATTFAQSRGFDTSRMDTSVEACTDFFQ
                     YANGTWLKNTEIPASQSRWGTFNILSDNNNTILREVLESAAKTRGAQGSDTQLIGDFY
                     ASCMDEAAIEKASASALEPFFRQIDKIKTVDDLQRQIALMHNSGLPALFGFGGGADLK
                     NSSMVIINAGQGGLSLPNRDYYTQSDAKSQDTRWKFAEYMTNMFKLLGDK"
BASE COUNT         1195 a         1497 c         1440 g         1378 t
ORIGIN      
        1 acaagcacgg aaaaagatat taacagggat gaagaggatg aacagggatt tttaaaggca
       61 aaagttaaaa ggtaaaattt agattttact ttctactttt cttatctcct tcatctcgtt
      121 tatccctgta aattcttttt atcaaagaat caagcgttta tcgcccgcgc gtcgcgtatg
      181 tttttcgcgg tcgaaatatt cgagcagcgg aatcgcgtat ttgcgcgaga ttccggcaat
      241 ttccttaaac gtcgccatat cgattaaccg gtcgggagtc tgcgcggcgt aatttttcaa
      301 ttgattgact agacggtcga gcgcttcgcg gcggaaataa aaatcttccg tgactttgac
      361 gatttccgcc gcgttcaaaa aaagctggaa aactttgcgc gcctgctggc ggtcgagttt
      421 tgttccgcga atcgcttcgt cgagcgcggc ttccagcgcc ggaacttcga gttttgcctg
      481 cgcgtaaatt ttttccagcc tttcgcgcag aattttttct tccgccgaaa gctccggatt
      541 atgcgcgacg gcgcgcacca aatctttttc cgccgctatc ctggcgtctt tttccagcgc
      601 cgccagaatc gctttgaaaa tttcggcggg caggcgcgca aagatttttt cgcgcagagt
      661 ttcgcgcccg atgccgcggg cgagcggttc gcggcggtga tgcgcttcga tttccgccaa
      721 agttttcgcc ttcagattat caaacggcgt tcgcgcgaca aaaaatccgc cggtttcgac
      781 gacggacttt ttctccgaat tttccgccag agcttttcgc aaaacgcttt cgcgccagcc
      841 cgtgcgcgcc tgcaaatcgg caaaacgagc gccgctctcg cccgccgttt cgagatagat
      901 tctcgcctgc cgggctttgt cgttccggtt ttcggcttca attaaatttt gcagaacgcg
      961 gcgggcgctt tccgtatctt tgcgccggtg tttcattgcc tgcgcgtcta aaacccgccc
     1021 gcccgcgatt gttacctgcg gcgagtagct gcgaatgata aaacgctcgc ccggaatcgc
     1081 cgccgccggc gcttcgagcc gtaattgaac aaaatctctg gcgcccgggg cgatttcgcc
     1141 cgcttcgttt aaaacctgaa ttctggctaa ggcttcgatt gttccgagat gagcgcggac
     1201 gcgctggcgc gattttaaag cccgtttcgc gtcttttaaa acttcgattt ccgcatcgaa
     1261 aatctgcgtc ggctgaagag cgtctttttc gcacagaacc attccgcgct caatctcggc
     1321 gtgctcgatg ccgcccagat tgacggctgt gcgctgtccg gcgtgggcgg ttttgacgga
     1381 ttttccgtga gtttgcaagc cgcgcacgcg cacttttctt ttgagaggca aaacttccag
     1441 ctcgtcgccc tcgttgattt cgcccgcgat taaagtcccg gtgacgaccg cgccgaaacc
     1501 tttgaccgta aaagaacggt caatcggcaa gcgggcgacg gtttcgtttt tcggcggcgg
     1561 aatctgcgcg gcgagatttt tgaaagcttc tttcagctcg tcgatgcctt cgccggtttt
     1621 ggagctgacg gcgatgacgg gcgcattttc gagaaaggag ttttcgacca gttcctgcgc
     1681 gtccagccgg accagttcga gcagctcggc gtcgaccata tccgttttcg tcaaaacgat
     1741 taaacccgct ttcgtttcca aaaggcggca gatgtcgaaa tgctcgcgcg tctgcggcat
     1801 cacgccttcg tccgccgcga tgaccagcgc caccaaatcg attccggacg cgcccgccag
     1861 catatttttg acaaattttt cgtgtcccgg cacatcgacg aaaccgatgc gaacgtcgcc
     1921 caaatccagt tcggcaaagc ccagatcaat cgtgatgccg cgtcgttttt cttccggcag
     1981 gcggtcggcg tcgatgcccg tcaaagcgcg cacgagcgag gtttttccgt ggtcgatgtg
     2041 tccggcggtg ccgacgataa tgtccataga cgtcacggaa aagtttaaag tccaaagcgc
     2101 atcctgtaaa gcagatgcgg cttcggactc gatgaatgaa atcgaattta ttgagtgacc
     2161 tgaaaatcaa cccattgcga aaccgtctga ttggtcagca aatccctgac gataatttgc
     2221 aaagcgtaat cgcccggctg catattcggg ctgagccgca tatagccgta atcagcgatg
     2281 cgcgtcatat cgtcctgctt ttcgagctgg ctggcttgcg gctgcccctg cgtgacgagt
     2341 tgaccgtcgc ggtaaagatt gacctgcacg gttaatttcg gctgatttgc ggctttgtcg
     2401 agcgcggcat tatagagcgt gtaggaataa gccaaaatca ttccgcgctg aaactgtcga
     2461 atcgccggaa cggatgtatt cagcgccgcg gcaatcgcgt tttccggttt gacctctgcc
     2521 ggcacgataa attttccgtc tgcatcgacc cggctgacgg tcaagccgga caaaaagagc
     2581 ttatcttttt tcagcgcggg aacctgtata atctggctcg ccgagccgag cattttgctg
     2641 ccggcgtcgc gtacggcgag acggaagttg taagttcctt cctttttcac cggcacgtcg
     2701 acgctgtaaa ccagaccgtt ctgcctgata agctcgaccg cctgtgccgg aatcagcacc
     2761 ttgtgagtgt ggttaaactc atcgacgaca tcgtttttct cgtccagcgt gacggcgaaa
     2821 acgtcgagcg ccactttttt cgagccgttc gcgtcgtcgg caaaagtaat atctttgccg
     2881 tccacgtgga cgagcgagcg cacgaaattt cctttcgccg gcgtatttcc gaaaaacgcc
     2941 gtcaggcgca gattcattcc cgttcgcgga agcggcgcga cgaccgcttc gtaaagctcg
     3001 ctgtcgcccg tctttcgact gacattctgc ttttcgtcga ctctgcccag aaaaccggcg
     3061 cgcgaaacca ctttcagacc gggacgactg accttgatgg agacctgatt gaaattcttt
     3121 cctttaaaag tttcgtcgtc gggcgcgtaa gcaagcaggt aatagccttt ttccagattc
     3181 agcgcgcgcc cgatgggcac gtccagaaaa ttttgatttt tggaaaacgt gccgcccgtt
     3241 tcgcgggcga gaaaattcag cccgtcctgc attcccaaca aaaggctccg gcgctctgcg
     3301 gcgactttat ccgtgctgcc gctggaaact tcgtcgaccg cctcaatcgc gtccgtatta
     3361 agcagtcccc gcgtgtcgat cgtattgaaa accacggctg cgcggtttgc cgcgtcggtc
     3421 aagccgcgca gcgcggcttc ggtgcggacg ctgtgcgttc cgagagattt gtcgctccgc
     3481 gtgcgcagcg gaatgccgtc ggacaaaaga aaaaccactt tgcgcccgcc gacgcgaccc
     3541 aatccgcgca cgatgtaatt gagcacgtta ataacgctga ccgattgaat atcgcggctc
     3601 gcgtcttccg ccgcttcctt tatctctctt tccgtttccg tcagaacatc gtcgcggacc
     3661 ggcgcgaaaa cgggtccgcc cgccgtcagg cagcctcgat tcggatacca gacgattttg
     3721 cggacgagcc ggagaagctg ttcgcggtct gaagtaaact gctgaagcag gctgctgccg
     3781 ccggtggtct ggtaaatcgc caccatatcg ccggcgagca tctgctcccg gacaaacttg
     3841 ttcagggctt cgcgcgcggc gttgatgccg ctgccggacg ccgtgcagtt atcgacgacg
     3901 aaagtaatca agcgtcctcc gggcgcgcgc cccgcaggcg gcggcggcag aacggcgttt
     3961 ttatcttttt tctcgatttt aaccggcgcg ctgctggagg cgaccgtgtt gacgtaggaa
     4021 aaattggtga ttttctgcgg tttgccgtcc tgccagactt caaaatcggc ggcggtcaaa
     4081 tccgtcacct gattgccttt ttcgtcggtc accactgcgt cgagctggac gagcttggtc
     4141 gtgattttga ccacgtcgtc ttcgtccggc ggcgtaggat tcgtttgctg cgcgaaaatc
     4201 gaaattgtcg tcagcaaaca gataactaaa gcgaaagaga actttttcat cccgaatact
     4261 ccaaaaagtt ataaacgata agtcggcttt ctcagcggcg ccgagacggt gcgggcaaat
     4321 tgctgtttct acgtatctta ctctgttgcg gcggcgggcg gcaagactgt tttgttttat
     4381 ttcattcgat tctcgcgtct gaaacgcaaa attcaaaacg caaattgcga aacggaaaga
     4441 caaacgcgat ttgaaagcag acaagcggcg tttgtctaaa gacaagtgac gtttgtctaa
     4501 agacaagtga cgtttgtctt cggacaaata gcgtttgtct ttagacaaat cgtgtttgtc
     4561 tttagacaaa tcgtgtttgt cttcagacaa gcggcgtttg tcttcggaca aatcgtgttt
     4621 gtccgcttcc aaatcgcgtt tgtctgaaga catttcacgt ttgcctttaa tcaaaccgcg
     4681 cttattcgtg ccgtgattat aggcgataaa gtggtaaacg gtaagcggta agctcgttat
     4741 tttgaatttc gagtttttat ttaaaattcg atattcaaca accgcaactt accgtttacc
     4801 gtttaccact ttaaaacttt tttcgtatac tctttaataa cgacaatcaa aatcttccaa
     4861 aatccgagag gtgacagaaa atgcgaactc ttcgttttat gaccgctttg accctattgt
     4921 tttcggtctg cgcgacgacc tttgcccaga gcagaggctt tgatacgagc cggatggaca
     4981 cttcggtcga ggcttgcacc gatttttttc aatacgcgaa cggaacgtgg ctgaaaaaca
     5041 cggaaattcc cgcttcgcag tcgcgctggg gcacttttaa tattctcagc gataacaaca
     5101 acacgattct cagagaagtt ctggaaagcg ccgccaaaac ccgcggcgca cagggcagcg
     5161 atacgcagct tatcggcgat ttttacgctt cgtgtatgga cgaagcggca atcgaaaaag
     5221 cgagcgcgag cgcgctcgaa ccctttttcc gacaaatcga caaaatcaag accgtcgacg
     5281 atttacagcg ccaaatcgcg ctgatgcaca acagcggctt gcccgcgctt ttcggtttcg
     5341 gcggcggcgc ggatttaaaa aattcgtcga tggtcatcat caacgccggt cagggcggct
     5401 tgagcctgcc gaaccgcgat tattacacgc agagcgacgc taaatcgcag gacacgcgct
     5461 ggaaattcgc ggaatatatg acgaatatgt tcaagctgct cggcgataaa
//
LOCUS       DASWNE010000398         1976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_13525, whole genome shotgun sequence.
ACCESSION   DASWNE010000398 DASWNE010000000
VERSION     DASWNE010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13525"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VGB68_08015"
     CDS             <1..433
                     /locus_tag="VGB68_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049214.1"
                     /translation="DTETRFLMNPGFQPIKGAEGWQLSNPPIFQLAALKASLDIFEEA
                     GMKALTEKSRRLTGYLEFLLGEIQDARISVITPSNPESRGCQLSIRVKDSDKNLFQKI
                     TAQGVIADWREPDVIRVAPVPLYNTFTDVYRFAEILQDCLK"
     gene            604..>1976
                     /locus_tag="VGB68_08020"
     CDS             604..>1976
                     /locus_tag="VGB68_08020"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0049215.1"
                     /translation="MSKQKVIIVGAGLSGSLLAIYLAKRGIEADVYEARGDMRKIEMS
                     AGRSINLALSDRGIAALREVGMDEYMLAEAVPMLGRMVHAVDGQTKLLPYSGRAGEYI
                     NSVSRAGLNVALINEAEKYEGVKFHFNEKCVRFDCQTGEASFENAAAGDKKSVKGDTL
                     IAADGAGSAIRGAMLHGGVPRFDFSQKFLEHGYKELHIPSRKDYHSAEAAGSDTGGDF
                     LIEKNALHIWARKAFMMIALPNFDGSFTCTLFLAHEGENSFEQLKDEKSLMKFFQTNF
                     ADAIPLMPTLTEDFFANPIGNLGTIKCFPWNVGGKSLLLGDSAHAVVPFYGQGMNASF
                     EDCRVLNRLIEKHDADWETIYKEYGAQRKENTDAIADMAEENFYEMRDAVADPVFVRK
                     RELETRLEQTFPEYFSKYSMVTFREDLPYSVAKQKGNAQDKLLMEICAKVDDISQIDL
                     NDVMEKV"
BASE COUNT          521 a          455 c          534 g          466 t
ORIGIN      
        1 cgatacggaa acgcggtttc tgatgaatcc gggtttccag ccgataaaag gcgccgaagg
       61 ctggcagctt tccaacccgc cgattttcca gctcgccgcg ctgaaagcct cgctcgatat
      121 ttttgaagaa gccggaatga aagctctgac ggaaaaatcg cgccgattaa ccggatatct
      181 ggaatttttg ctcggcgaaa ttcaggacgc aagaatttcc gtcatcactc cttctaatcc
      241 ggagagccgc ggctgccagc tttcgattcg cgtcaaagat tcggacaaaa atctttttca
      301 aaaaattacc gcgcagggcg tcatcgccga ctggcgcgag ccggacgtta tcagggtcgc
      361 gcccgtgccg ctttataata cttttacgga cgtttatcgt ttcgcggaga ttttgcagga
      421 ttgtctgaag taatttcggg gtaataaacg cgcgctgatt tttccgggaa aaagtatatg
      481 ttctatttgt gacgacacat ttataacgaa acggtcgcaa cgattatttg gggaaaagga
      541 aacttatcat cggatggaaa acgacggaag cgtttccgtt ttaaaaaaag aaatttgatt
      601 tttatgtcga aacaaaaagt aatcatcgtc ggcgcgggtt tgtcgggttc tcttctggcg
      661 atatatcttg ccaaacgcgg tatcgaagcc gacgtttacg aagcgcgcgg cgatatgcga
      721 aaaatcgaga tgtctgccgg gcgctcgata aatctcgctt tgtcggatag aggaatcgcc
      781 gctctgcgcg aggtcgggat ggacgaatat atgctggcgg aagccgtgcc gatgctggga
      841 cgaatggttc acgccgtcga cggtcagacg aagcttctgc cgtatagcgg gcgcgcgggc
      901 gaatacatca actcggtttc ccgcgccggt ttgaacgtcg ctttgataaa cgaagcggaa
      961 aaatacgaag gcgtcaaatt tcatttcaac gaaaaatgcg tccggttcga ttgtcaaacg
     1021 ggcgaagcgt cgtttgaaaa cgcggcggcg ggcgataaaa aatcggttaa aggcgatact
     1081 ttaatcgctg ccgacggcgc gggctcggcg attcgcggcg cgatgctgca cggcggcgtt
     1141 ccgcgttttg atttctcgca aaaatttctt gaacacggct acaaggaatt gcatattcct
     1201 tcgagaaaag attaccattc ggcggaggcg gcgggttctg acacgggcgg cgattttctg
     1261 atagagaaaa acgcgctgca catctgggcg cgaaaagcgt ttatgatgat tgcgctgccg
     1321 aatttcgacg gcagttttac ctgcacgctt tttctcgcgc acgaaggcga aaacagtttc
     1381 gagcagctca aagacgaaaa atccctgatg aaattctttc aaacgaattt tgccgacgcg
     1441 attccgctga tgccgacttt gacggaagat tttttcgcca atccaatcgg aaacttaggc
     1501 acaatcaaat gttttccgtg gaacgtcggc ggcaaaagcc tgcttttggg cgattcggcg
     1561 cacgccgtcg ttccgtttta cgggcaggga atgaacgctt cgtttgaaga ctgccgcgtt
     1621 ttaaaccggc taatcgaaaa acacgacgcc gactgggaaa cgatttacaa agaatacggc
     1681 gcgcagcgaa aagaaaacac cgacgcgatt gccgatatgg cggaagaaaa tttttacgag
     1741 atgcgcgacg ccgtcgccga cccggttttc gtgcgaaaaa gagagctgga aacgcggctc
     1801 gaacaaacct ttcccgaata tttttccaaa tattcgatgg tcactttccg cgaagatttg
     1861 ccttattcgg tggcgaaaca aaagggcaac gcgcaggata aattgctgat ggaaatctgc
     1921 gcgaaagttg atgacataag ccagattgat ttgaatgatg taatggaaaa agtaca
//
LOCUS       DASWNE010000399         6594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_365596, whole genome shotgun sequence.
ACCESSION   DASWNE010000399 DASWNE010000000
VERSION     DASWNE010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6594
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365596"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VGB68_08025"
     CDS             <1..369
                     /locus_tag="VGB68_08025"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY0049216.1"
                     /translation="RNPLADTTGNFLRSVPMIILVALPFVYRIHLSPKGIVLAVLSGA
                     ISSGIGYSLWYAALKYHTATRAAILQLSVPALAAIGGVIFLAEIISMRLWLASALILG
                     GIALAILGKRKPRIHADKRV"
     gene            complement(366..1010)
                     /locus_tag="VGB68_08030"
     CDS             complement(366..1010)
                     /locus_tag="VGB68_08030"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0049217.1"
                     /translation="MTDAFESMDRMYRHQRYFYDLTRKYYLLGRDRLLAEINVQAGEN
                     VLEVGCGTGRNLIILAKRFPKANFFGLDASSEMLKTAQAKIQTGNLKNAALRTALADT
                     FDYRETFGLAEPFDTIFFSYSISMIPVWRESIQNALANLKSGRSFYIVDFYDQNDLPR
                     WFQKFLKSWLKKFHVQFWGDLMPYLEDLEKRGVGKLKINPVARRYAFIAQFRKN"
     gene            complement(1126..2370)
                     /locus_tag="VGB68_08035"
     CDS             complement(1126..2370)
                     /locus_tag="VGB68_08035"
                     /inference="COORDINATES: protein motif:HMM:NF023327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtaA family protein"
                     /protein_id="HEY0049218.1"
                     /translation="MNPEQDLVLGAVQNEKTTSKQGVLQKLFAVWFDAFVYNQIWEDP
                     RVDLQALRLNEKSRILTISSGGCNALNYLVENPERVTAVDLNRHHIFLLNLKIAALEF
                     LPEHEDFFAFFGYGKSADSVRNYQKFIAPNLDAATREFWESNSFIGRIAHRKRIDFFR
                     KGGLYEHSRNGYFLRFFHKFSRLLGCKPEELLKAETPEEQARLYEKYIAPFFDSALIK
                     TIGKMPVTMFGLGIPPQQYDELKKDLADGGSLIDVYKERAKRLAVEYPIYENYFAWQA
                     FARKYDTVNRLAVPEYLKKENYEALKKNAGKLRTGIGSITEEIKRQPAAAFNRFVFLD
                     AQDWMNAGTMTDLWSSIAEKGEKGSRIIFRTAGAASPVETNLPKDLRAKFVYEEDFSK
                     KLFKQDRASIYGGFHLYELTGS"
     gene            complement(2433..2825)
                     /locus_tag="VGB68_08040"
     CDS             complement(2433..2825)
                     /locus_tag="VGB68_08040"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY0049219.1"
                     /translation="MPGLNLFLRVLVGLGAFVSTIFVLLSMYTTITERRKEIGILKSL
                     GASKTFIISTIEGEAFLIGVLGILLGLAVSFLTAFLIGESFELAFEFSPNWILTAILI
                     AVGGSLVGALYPAWQASGIDPVEVMVNE"
     gene            complement(2898..3530)
                     /locus_tag="VGB68_08045"
                     /pseudo
     CDS             complement(2898..3530)
                     /locus_tag="VGB68_08045"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(3537..4916)
                     /locus_tag="VGB68_08050"
     CDS             complement(3537..4916)
                     /locus_tag="VGB68_08050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0049220.1"
                     /translation="MNQKLLKSVHLTNYYHKNSGGISTSFNNLLAAAARHRRLVRLIV
                     PGETEAVEEINDYAKIYYVPAKYSPIFDKRYRLIMPWQYMQGDSLIRKILLEEKPDLI
                     EVTDKYTISMLGAMIRLGKFRQLGRPALVHFSAERMDDNIASFMTKGALGGWLARRVI
                     GNYTLAVFDFHIANSVYTAQEFYNAYKKAENSSRSDWFLNKCWQFFKAPRIAIEERIY
                     VCPRGVNAETFRADRKSEAVKGEMRERANIPENAIVLLYAGRISPEKNIGLLVEMMKI
                     LARDAEKDFRLLVAGAGPEADWLQQETNRNFPGKIIQLGHLDKEALADYYANCDVFVH
                     PNPREPFGIAPLEAMASGAPTLAPNSGGILSYATGENAWLVEPTGENFAAAVSEIIEN
                     PELRERKVKNAVKTALENTREKSTDNLFATYDRMYEDFQKRKDLFTDIEATKNFDFVR
                     EVLTADERR"
     gene            complement(4913..5725)
                     /locus_tag="VGB68_08055"
     CDS             complement(4913..5725)
                     /locus_tag="VGB68_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049221.1"
                     /translation="MTFEETPSAVPQSNTISEAWTNLLRNPAQFIRYWNYKGAIMSGV
                     LRAPIFLVTYLVGRESIKLALGAALAQFVFRFLFAGVGGALIQSFRRVEPPWKALATI
                     LLVVPVISHIFEFLVQWSFAYLTATNDHTDEAIVRSICVSIISALFTLFIMRRNVMIV
                     GEAESKSLFSDLARIPALIFHFVAFIPNEISSLLRRGALIPVGLSIVGFGFFSQMLCW
                     AVTNKAFWTYGGGRSIPLLKFWGLDGAVLLILAVALSSYVFDRQRKRNISLS"
     gene            complement(5842..>6594)
                     /locus_tag="VGB68_08060"
     CDS             complement(5842..>6594)
                     /locus_tag="VGB68_08060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0049222.1"
                     /translation="KPITNFAERKIMDGAVLYYKMPKVILAPNQELLDALTKGTGRIG
                     HLMLRGVDTEVYSPAKRTVNDNILRFGFVGRLRAEKNVRLLADLEKRLLEAGKSNFKF
                     LIVGEGNEREWLEKNMKNVEFTGFLEGARLSEAYANIDVFLFPSETDAFGNVVQEANA
                     SGVPAIVTNQGGPKFIVKHAETGFVSENLDDFVKYSIELMDNPAKLEKMKEASRRAAL
                     SRSWDSVFETVYDAYAEAIKISEERKQKTGEN"
BASE COUNT         1544 a         1758 c         1656 g         1636 t
ORIGIN      
        1 cggaatcctt tagccgatac gacggggaat tttctgcgct cggtgccgat gataattctg
       61 gtcgcgctgc ctttcgttta ccggattcat ttatcgccga agggaatcgt cttagccgtt
      121 ttatcgggcg cgatttcgtc gggcatcggc tattcgctct ggtatgccgc gctgaaatat
      181 cacacggcga cgcgcgcggc gattttgcag ctttccgttc cggcgctggc ggcaatcggc
      241 ggcgtgattt ttctggcgga aattatttcg atgcgtttat ggctggcgag cgcgctgatt
      301 ctcggcggga ttgctctggc gattttagga aaaaggaaac cgcggattca cgcggataaa
      361 cgcgtttaat tctttcggaa ttgcgcgatg aaggcgtagc gccgcgcgac cggattgatt
      421 ttcagttttc cgacgccgcg tttttccaaa tcttccagat acggcatcaa gtcgccccag
      481 aattgaacgt gaaatttttt cagccagctt ttcaaaaact tttgaaacca gcgcggcaaa
      541 tcgttctggt cgtaaaaatc cacgatgtaa aatgagcgcc cgctttttaa attagccagc
      601 gcgttttgaa tcgactcgcg ccagacggga atcatcgaaa tcgagtaact gaaaaaaatc
      661 gtgtcgaaag gctcggctaa accgaaggtt tcccggtaat caaaagtatc ggcgagcgcc
      721 gtccgcagcg ccgcgttttt caaattaccg gtttggattt tcgcctgcgc cgttttgagc
      781 atttcggacg aggcgtcgag cccgaaaaaa ttcgctttcg gaaaacgctt cgccagaata
      841 attaaatttc tgcccgttcc gcagccgact tcgagaacgt tttcgcccgc ctgcacattg
      901 atttccgcca gcagacggtc gcgccccaaa agataatatt tccgtgtcaa atcgtaaaag
      961 taccgctggt ggcgatacat tctgtccata ctttcgaaag cgtcggtcat atttagaagt
     1021 tgcagaaagc tgcaggaagt tgcaggaagt taacgggaag ttgcggatag ttgcaggaag
     1081 ttttgatatg taaccggctt ttttcaaact atccgcaact tcccgttaac ttcccgtcaa
     1141 ctcgtataaa tgaaatccgc cgtaaatcga ggcgcggtct tgtttgaaaa gctttttgga
     1201 aaagtcttcc tcgtaaacga atttcgcgcg taaatctttc ggcaggttcg tttcaaccgg
     1261 cgacgccgcg cccgccgtcc gaaaaattat ccgcgagcct ttttcgcctt tttccgcgat
     1321 tgacgaccat aaatccgtca tcgtcccggc attcatccaa tcctgcgcgt cgagaaaaac
     1381 gaagcggttg aaagcggcgg cgggctggcg tttgatttct tcggtaatcg agccgattcc
     1441 ggttcgcagc tttccggcgt ttttcttcag agcctcgtaa ttttcttttt tcagatattc
     1501 cggcacggca aggcgattca ccgtgtcgta ttttcgcgca aacgcctgcc acgcaaaata
     1561 attttcgtaa atcggatatt cgacggcgag acgtttcgct ctttctttat aaacgtcaat
     1621 taggcttccg ccgtcggcta aatctttttt cagctcgtcg tattgctgcg gcggaatgcc
     1681 gagcccgaac atcgtcaccg gcattttgcc gatggttttg atgagagcgg aatcgaaaaa
     1741 aggcgcgatg tatttttcgt aaagccgcgc ctgttcttcg ggcgtttccg cttttaaaag
     1801 ctcctcgggc ttgcagccca gaagccgcga gaatttatga aagaagcgca gaaaataacc
     1861 gttgcgcgag tgctcgtaaa gcccgccttt gcggaaaaaa tcgatgcgct ttcggtgagc
     1921 aatcctgccg ataaaagaat ttgattccca gaattctctg gtcgccgcgt ccagattcgg
     1981 cgcgataaat ttttgataat ttcggacgct gtccgcgctt ttgccgtaac cgaaaaaggc
     2041 gaaaaagtct tcgtgttccg gcagaaattc gagcgcggcg atttttagat tgagcagaaa
     2101 aatatgatga cgattcaggt cgaccgccgt cacccgttca ggattttcga ccagataatt
     2161 cagcgcgtta cagccgccgg aagaaatcgt cagaatgcgc gatttttcgt tcagccgtaa
     2221 agcctgcaaa tcgacgcgcg ggtcttccca gatttgattg taaacaaacg cgtcgaacca
     2281 gacggcgaaa agcttctgca aaacgccctg tttgctggtg gttttttcgt tctggacagc
     2341 gcccaaaact aaatcttgtt ccggattcat aaagaaaacg cgtgaacgcg caatgatgaa
     2401 ttcatcgttc cgcattcatc ttttcaaata ttttactcgt taaccattac ctcgaccggg
     2461 tcgatgcccg aagcctgcca cgccggatag agcgcgccga cgaggcttcc gccgaccgcg
     2521 atgagaatcg ccgtcagaat ccagttcggg ctgaattcaa acgccagctc aaaactttcc
     2581 ccgataagaa aagccgtgag aaacgaaacc gccagaccga gcaggatgcc gagcacgccg
     2641 attaaaaacg cctcgccttc gatggtcgag atgataaagg ttttcgacgc gccgagcgat
     2701 tttaaaatgc cgatttcctt tcgtctttcc gtaatcgtcg tatacatcga aagcaatacg
     2761 aaaatcgtgc tgacaaacgc gcccaagccc acaagcacgc gcaggaaaag attcaagccc
     2821 ggcacgcgtt cctgcgcgtc gatgattaaa tcgcgcgtca aattgacttt gttgcccggc
     2881 aaggtttcat tgattcgggc ggcgacttct tcgacgcgcg cgccgtcttt cagctttact
     2941 aaaatgtatg tgcatttatt cggcgcttcg agcgcgtcct gcatcgcggc gagcgacatt
     3001 tttactctgg cgcccgacgg cggcgcgaaa attccgaccg ccgtgtattg ttttttgaaa
     3061 agctcgattt tatcgccgag cctgacgttt tcgtccttta actagcgttc gtcgagaatc
     3121 acctcgtcag cggcgacggc ggcgcgcccc tcgagaatct gcatctcgtt catttcggca
     3181 aacggctgcc attcgacgcc gtcaatttgc ataattcccc agcggtggct cggattcggc
     3241 gtgatgtagc gaatcacggg aacggtcgcc tcgacgcctt cgatttgctt taatctctcg
     3301 gcgtaagcgg tcgaaaccga agcgttcgag ctggcaagct ccatcgcgcc cggacgcgtg
     3361 aaaacaattt ccgctttcca gtttgaagcc cgccgcgcca tatcattcgt cattccgcgc
     3421 gccagacccg taaacaaaat aacgagaacc acgcccaatg cgacgccgac gacgctaatc
     3481 aacgtgcgaa aaggacgagt ttttaaattg gctaaaacta attctaacat ctgcgtttat
     3541 ctgcgctcat cggcggttaa aacttctcgc acaaaatcga aatttttcgt cgcctcgata
     3601 tcggtaaaca aatctttgcg cttctggaaa tcttcataca ttcggtcgta agttgcaaac
     3661 aggttgtcgg tcgatttttc gcgcgtgttt tccagcgcgg tcttgacggc gtttttcact
     3721 ttgcgctcgc gcaattcggg attttcgatg atttcggaaa cggcggcggc aaaattttcg
     3781 cccgtcggct cgacgagcca ggcattttcg ccggtcgcat acgataagat tccgccggag
     3841 ttcggcgcca gcgtcggagc gcccgacgcc atcgcttcga gcggcgcgat gccgaacggc
     3901 tcgcgcggat tcggatgcac gaaaacgtcg cagttggcgt aataatccgc cagagcttct
     3961 ttgtctaaat gccctaattg aatgatttta ccgggaaaat tccggttggt ttcctgttgc
     4021 agccaatccg cttcgggtcc cgcgcccgcg accagcaggc gaaaatcctt ttccgcgtct
     4081 cgcgccagaa tcttcatcat ttcgaccagc aagccgatgt ttttttccgg cgagatgcgt
     4141 ccggcgtaaa gcagcacgat tgcgttttcg ggaatgttcg cgcgctcgcg catctcgcct
     4201 ttaacggctt cggatttacg gtcggcgcga aaagtttcgg cgttgacgcc gcgcgggcag
     4261 acgtagattc tttcttcgat ggcgatgcgc ggggctttaa aaaactgcca gcatttattt
     4321 aaaaaccaat ccgagcggct cgagttttcc gcttttttgt aagcgttata aaattcctgc
     4381 gccgtgtaaa ccgagttagc gatgtgaaaa tcgaaaaccg ccagcgtgta attgccgatg
     4441 acgcgccgcg ccagccagcc gccgagcgcg cctttcgtca taaacgaagc gatgttgtcg
     4501 tccattcgct cggcgctgaa atgcacgagc gccgggcgtc cgagctggcg aaatttgccc
     4561 agccgaatca tcgcgccgag catcgaaatc gtatatttat ccgtgacttc gattaaatcc
     4621 ggcttttcct caagcagaat tttgcggatg agcgaatcgc cctgcatata ttgccagggc
     4681 attatcaggc ggtagcgttt atcgaaaatg ggcgagtatt tggcgggaac gtaataaatt
     4741 ttggcgtaat cgttgatttc ttcgaccgct tcggtctcgc ccggaacaat caggcggact
     4801 aatctccgat gacgcgcggc ggcggcgagc aggttgttaa agctggtgct gatgccgccc
     4861 gaatttttgt gataataatt ggtcaggtga accgatttta aaagcttttg attcacgata
     4921 aacttatatt tcgttttctc tggcggtcga aaacataaga cgaaagggcg accgccagaa
     4981 tcagcaaaac agctccgtca agcccccaaa atttcaataa cgggatgctg cgtccgccgc
     5041 cgtatgtcca gaaggctttg ttcgtcaccg cccagcacag catctgcgag aaaaagccga
     5101 acccgacgat gcttagaccg acgggaatca gcgcgccgcg ccgcagcagc gacgagattt
     5161 cgttggggat aaaagccacg aaatgaaata tcaaagccgg tatgcgcgcc aagtcgctga
     5221 aaagcgattt gctttccgcc tcgccgacaa tcatcacgtt gcgccgcata ataaataaag
     5281 taaataaagc cgagataata gaaacgcaaa tcgagcgcac gatagcttcg tccgtgtggt
     5341 cgttcgtcgc cgtcagatac gcaaagctcc actggacgag aaactcgaaa atatggctga
     5401 ttaccggcac taccagtaaa atcgtagcca gcgccttcca cggcggttcg acgcgccgga
     5461 acgactggat taaagcgccg ccgacgccgg caaataaaaa acgaaaaacg aattgtgcga
     5521 gcgccgcgcc cagcgcgagc ttgatgctct ccctgccgac caggtacgtc accagaaaga
     5581 tgggcgcgcg caaaacgccg ctcattatcg cgcccttgta attccaatac cggataaact
     5641 gcgccgggtt gcgcagaaga tttgtccaag cctcgcttat cgtattcgac tgcgggacgg
     5701 cggacggcgt ttcttcaaaa gtcatttcct tcatactgct tcggacaagc gcgcgacgcg
     5761 taaaaatttt ctatcgcttt cttagagctt ggcggcagct gcgagccaaa taaaaaatgt
     5821 tacggactaa ataaaaataa atcaattctc gcccgtcttt tgttttcgct cctccgaaat
     5881 ttttatcgct tcggcgtaag cgtcgtagac ggtttcgaac accgaatccc agcttcgcga
     5941 cagcgccgcc cggcgcgaag cctctttcat tttctctaat ttcgcgggat tgtccatcag
     6001 ctcaatcgaa tatttaacaa aatcgtcgag gttttcggag acgaatccgg tttcggcgtg
     6061 cttgacgata aatttcggac cgccctgatt cgtcacgatt gccggaacgc ccgaagcgtt
     6121 cgcttcctgc acgacgttgc cgaaagcatc ggtctccgaa gggaaaagaa aaacatcgat
     6181 attggcgtag gcttccgata agcgcgcgcc ttcgagaaag cccgtaaatt cgacgttttt
     6241 catattcttt tcaagccatt cgcgctcgtt gccttcgccg acgattaaaa atttaaaatt
     6301 gcttttgccc gcttcgagca ggcgtttttc caaatcagcc agcaggcgca cgtttttttc
     6361 ggcgcgcagc cgcccgacaa agccgaaacg caggatattg tcgttaaccg ttcgctttgc
     6421 gggcgaataa acttcggtgt ccacgccgcg cagcatcaaa tgcccgattc tgcccgtgcc
     6481 cttcgtcagc gcgtcgagca attcctgatt cggcgcgaga atgactttcg gcattttgta
     6541 gtaaagcacc gcgccgtcca taatttttcg ctcggcaaaa ttcgttatcg gttt
//
LOCUS       DASWNE010000400         1611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_82937, whole genome shotgun sequence.
ACCESSION   DASWNE010000400 DASWNE010000000
VERSION     DASWNE010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82937"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..1174
                     /locus_tag="VGB68_08065"
     CDS             209..1174
                     /locus_tag="VGB68_08065"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0049223.1"
                     /translation="MNLTSKVLVTGGAGFIGSNLADALIRQGAKVTIIDNLATGFREN
                     LEEIKGDFEFVEGDLNDDSALQKALDGAEIVFHQAALPSVPRSVDNPQETHSACVNAT
                     FNLLLKARDAGVRRLIYAASSSAYGNQATLPKVESMRPEPLSPYAAAKLMGEYYCQVF
                     SNVYNLETISLRYFNVFGPRQNPSSMYSGVISRFIDALMSGKTPIIYGDGEQSRDFTF
                     VANVVNANIKAAQTTKGIGQVMNCSNGERISLNQLLEVLKKITGKSDVQAEYELERAG
                     DVKHSQADNSRAKECLNYEKLVGLQEGLKNTIDWWQQSRFAANNS"
     gene            1289..1364
                     /locus_tag="VGB68_08070"
     tRNA            1289..1364
                     /locus_tag="VGB68_08070"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1322..1324,aa:Lys,seq:ttt)
BASE COUNT          430 a          360 c          386 g          435 t
ORIGIN      
        1 gctcgatatg gaaaaagtca aaagtcttct gaaatcgccg aagattgccg acctgcgcaa
       61 tatttacgag ccgaaagata tgcgcgagct gggctttgat tatgtcggcg tcggtcgcta
      121 atttgcagtt ttaacgcaaa cggttaaaat gattaggcgg aaggttttaa agcttccgcc
      181 tttcttatct ttccttttta aattgaatat gaatttaaca tctaaagttt tagtcaccgg
      241 cggcgcgggc tttatcggtt ctaatctggc ggacgcgctt attcgccagg gcgcgaaggt
      301 cacgattatc gacaatctag ccacgggttt tcgtgaaaat ctcgaagaaa tcaaaggcga
      361 tttcgaattt gtcgaaggcg atttaaacga cgattcggct ttgcaaaaag ctctcgacgg
      421 agccgagatt gtttttcatc aagccgcttt gcccagcgtt ccgcgctcgg tcgataatcc
      481 gcaggaaact cactcggctt gcgtcaacgc gactttcaat ctgcttctga aagccagaga
      541 cgcgggcgtc cggcgcttga tttacgcggc ttccagctcg gcttacggta atcaggcgac
      601 tctgccgaaa gtcgagtcga tgcgcccgga accgctctcg ccatacgcgg cggcaaaact
      661 gatgggcgaa tattattgtc aggttttcag caacgtctac aacctggaga cgatttccct
      721 gcgctatttc aatgttttcg gaccgcgcca gaatccgtcg tcgatgtatt cgggcgtaat
      781 ttcacgcttc atcgacgcgc tgatgagcgg caaaacgccc atcatttacg gcgacggcga
      841 gcagtcgcgc gattttacgt ttgtcgccaa tgtcgtcaac gccaatatta aagccgcgca
      901 gacgacaaaa ggcatcggtc aggtaatgaa ctgctcgaac ggcgagcgca tcagcctcaa
      961 ccagcttctg gaagtgttga aaaaaataac cggcaaatcc gacgtgcagg ctgaatacga
     1021 gctggaaaga gccggcgacg tcaaacattc gcaagccgat aattcgcgcg ccaaagaatg
     1081 tctgaactac gaaaaactcg tcggtctgca agaaggcttg aagaatacga ttgactggtg
     1141 gcagcaaagc cgtttcgccg cgaataattc gtaatcgaga aatttttcac acgaaatcga
     1201 cttgccaact taaaattcaa tgtgtatgat ttttatttcg gttagtgaaa atatttcgct
     1261 aatgcgatac gttcttttct aaaaaagtgg tccgatagct cagtcggtag agcaaatggc
     1321 ttttaaccat tgggtcgtag gttcgagtcc tactcggatc accatttatt tcaataagaa
     1381 aagggtaacc gttttggttg cccttttttg tttgtgtgcg tggattgtgc caattttata
     1441 atattgattt atactgtctc gaaaatgacc ctttttgaga ctttcgcaaa gggcggaagc
     1501 cttaatgatt taaaaataga ttgtttttat ttgatgaata aaatagaagc caaatcgatt
     1561 ttagataccg gatacattta taaaacttca atgcgattac cggcgcattg a
//
LOCUS       DASWNE010000401         1907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_13748, whole genome shotgun sequence.
ACCESSION   DASWNE010000401 DASWNE010000000
VERSION     DASWNE010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13748"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1799)
                     /locus_tag="VGB68_08075"
     CDS             complement(<1..1799)
                     /locus_tag="VGB68_08075"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEY0049224.1"
                     /translation="MQDFRRLLKYLRPHSLTFALAILAMIFVAVFETATGALIVPIFD
                     QAFGTNTAQKTPTLFNLQNLIPVDDWFGAWLMISSLLIGFTLLGGVAEYFSVYLMSKI
                     GQSAVLRMRQDLYEHLLKQSAAFFERHRTNFLVSRLVVSATAIELAVSAHLRDVLREG
                     VALVFFLAAAFYLNWRLMLGALVIAPVIAFITSKFSKTLRRLSIESFEGNKLLTDTAQ
                     ESLANQSIVKAYRAEAREQERFTKVARLIARANFRSGKISAFSPPTISIIGVVAISFL
                     MFFGLREINLGRMEASQFFTFLFFLLRSYDPMRKISRLHNELNKAFAATRDIWDVMDQ
                     NDSMPEKADAVELPPLQNKIELRNVSFNYQTDNKQILRDINLAIPKGEMVALVGESGG
                     GKSSLIKLIQRLYDPSEGACFWDETDLRDASLDSLKKQIALVTQETVLFNDTIRYNIS
                     YGKPDASQAEIAEAARIAFAHDFIGELPNGYETIVGERGIFLSGGQRQRIAIARAVLV
                     DAPVLILDEATSALDAESERLVQKALANLMRNRTSIVIAHRLSTVRRADKIVVMERGR
                     IIETGTHAQLLAKGGTYRRLYELQFAEEEEEELA"
BASE COUNT          464 a          507 c          524 g          412 t
ORIGIN      
        1 gctaattctt cttcttcttc ctcggcaaat tgcagctcat acaagcggcg ataagtgccg
       61 ccttttgcca gaagctgcgc gtgcgttccg gtttcgataa ttcgcccgcg ttccatcacc
      121 acgattttat cggcgcggcg gacggttgaa agccggtgcg cgatgacaat cgaagtgcgg
      181 tttcgcatca ggtttgccag cgctttctgc acgagtcgct cgctttcggc gtccagcgcg
      241 ctggtggctt cgtccagtat taaaaccggc gcatcgacca gcacggcgcg ggcaatcgcg
      301 atgcgctggc gctgaccgcc ggaaagaaaa attccgcgct cgccgacaat cgtctcgtag
      361 ccgttcggca gctctccgat aaaatcgtgc gcaaaggcga tgcgcgccgc ttcggcgatt
      421 tccgcttggc tcgcgtcggg tttgccgtag gaaatgttgt agcgaatcgt atcgttaaaa
      481 agaacggttt cctgcgtgac gagcgcgatt tgctttttga gcgaatcgag cgaagcgtcg
      541 cgcaaatccg tctcgtccca gaaacaagcg ccttccgacg ggtcgtaaag acgctgaatc
      601 aattttatca ggctcgattt cccgccgccg ctctcgccga ccagcgcgac catctcgcct
      661 ttcggaatcg ccagattgat gtcgcggaga atttgtttat tgtccgtttg ataattgaag
      721 gagacgtttc ggagctcgat tttgttttgc agaggcggca attcgacggc gtcggctttt
      781 tccggcatcg aatcgttttg gtccattacg tcccagatgt cgcgcgtcgc ggcaaacgct
      841 ttgttcagct cgttgtgcag gcgcgaaatt ttccgcatcg ggtcgtagct gcgcaggaga
      901 aagaacaaaa acgtgaaaaa ctgcgaggct tccattctgc cgagattgat ttcgcgcaag
      961 ccgaaaaaca tcaaaaagct gatggcgacg acgccgataa tcgaaatcgt cggcggcgaa
     1021 aaggcggaga ttttgcccga acgaaaattg gcgcgcgcaa tcagccgcgc gactttggta
     1081 aatctttcct gctcgcgcgc ttcggcgcga taggctttga cgattgattg attggcgagc
     1141 gattcctgcg ccgtgtcggt cagaagcttg ttgccttcaa aagattcaat cgagaggcgg
     1201 cgcagcgtct tggaaaattt cgaggtgata aaagcgatga ccggcgcgat gaccagcgcg
     1261 ccgagcatca gccgccagtt cagataaaac gcggcggcga gaaaaaaaac gagcgccacg
     1321 ccctcgcgca aaacgtcgcg caggtgagcc gaaaccgcca attcgatcgc cgtcgcgctg
     1381 acgaccagcc gcgaaaccag aaaattcgtg cgatgacgtt caaaaaaagc cgccgactgt
     1441 ttcaacagat gctcgtataa atcctgacgc atccgcaaaa cggctgactg accgattttc
     1501 gacatcaggt aaacgctgaa atattcggcg acgccgccga gaagcgtaaa gccgatcagc
     1561 aaactggaaa tcatcagcca cgctccgaac cagtcgtcga ccggaatcag attttgcaga
     1621 ttgaaaagcg tcggcgtttt ctgcgccgta ttggtgccga aagcctggtc gaaaatcggc
     1681 acgatcagcg cgcccgtcgc ggtttcaaaa acggcgacaa aaatcatcgc cagtattgcc
     1741 agcgcaaagg tcagcgagtg cgggcgcaga tattttaata aacgtcgaaa atcctgcatt
     1801 gcgaaaaatt aaattattca cgttagattg cgccgacgca aggcgagaac cgggaaataa
     1861 agaattgacg agtctgaaaa gacgaataaa cgaatggctt tattcat
//
LOCUS       DASWNE010000402         3341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_438469, whole genome shotgun sequence.
ACCESSION   DASWNE010000402 DASWNE010000000
VERSION     DASWNE010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438469"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..892)
                     /locus_tag="VGB68_08080"
     CDS             complement(149..892)
                     /locus_tag="VGB68_08080"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0049225.1"
                     /translation="MKENQQPIVEFRRVSFEINGARILADLSLQIFRGETLVLLGESG
                     CGKTTTLKLINRLYEPTAGEVLVHGKSTIDWDAIKLRRQTGYVLQEAGLFPHFTIERN
                     VALVPDLENWNAEKKRARTAEMLELVGLKPEIFAARFPHELSGGQRQRVGVARALAAN
                     PELLLLDEPFGALDAITRTNLQKEFAGLVRELGKTAVFVTHDLQEAFVLATRICLMDK
                     GRIVLLDTPENFMKSDLPLARAYLETVNL"
     gene            1013..1564
                     /gene="mug"
                     /locus_tag="VGB68_08085"
     CDS             1013..1564
                     /gene="mug"
                     /locus_tag="VGB68_08085"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF007570.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HEY0049226.1"
                     /translation="MKDFKPSKESLRDAVNRTTEDLIDYNLKVLFCGINPGIWSGATG
                     FHFAKPGNRFWKVLHLAGFTNRLLHPSEEHELLALGYGITSLVKRTTARADELAVEEF
                     VAGGKLLVKKIDEYKPQNLAVLGIGAYRTAFNKPKAKLGLQEDKIGGARVWLLPNPSG
                     LNAHYQVDDLAALFGELRKTVEK"
     gene            1649..2359
                     /locus_tag="VGB68_08090"
     CDS             1649..2359
                     /locus_tag="VGB68_08090"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0049227.1"
                     /translation="MRLNAFVSDADFAGKLPIVIVQGLGISASYMIPAMSEMAKYAKV
                     FALDLPGFGDSEKPLHIFNIAELADVVADWMSASGIERAVLLGHSFGSQIAAEFALRH
                     PEMIERIVFAAPTFDRHARSFFRQFGRLLLNSRNEPFSLIMLAVKSYFKFGIRREART
                     LKFALADRIEDKLPRIGIPALVVRGELDTVVPQGWAEEVADLLPDGKLAVIAGGTHGV
                     NYQSPREFAAAIREFLDD"
     gene            2410..2664
                     /locus_tag="VGB68_08095"
     CDS             2410..2664
                     /locus_tag="VGB68_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049228.1"
                     /translation="MASEWAEEADRQAKAMTRDLIEVGRTKGFRVDYQKREARFMLRA
                     GTVITYFSAHDYETPKGMDWLQLRIDVLKQVRQAAPPEEK"
     gene            2765..>3341
                     /locus_tag="VGB68_08100"
     CDS             2765..>3341
                     /locus_tag="VGB68_08100"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 3'-phosphotransferase"
                     /protein_id="HEY0049229.1"
                     /translation="MNPDFPQTSLPTPLAQMISGYNRRQNRTGFSAARVFRLDAENKD
                     SLYLKIAPRTPARSLLQEKTKLEWLKNRLPVPEVLSFAEDENADYLLLSAISGVDASD
                     DSLKNDIPRIIEQLTDGLKMIHALPLESCPFDESLDYKIELAEKMMKDNLVDETDFDE
                     INQGKTAADLFRELIENKPAAADSVFTHGDYC"
BASE COUNT          846 a          853 c          867 g          775 t
ORIGIN      
        1 taaataataa tgaagaagtc aatctcggca tcacgctcga ttccgaaaac gtgacaatgg
       61 ggattgtgga tacaatgcct tcaattcaat tttattctcc ggcgctgctc aatcttccaa
      121 tcaatgtcag aaaacccgaa aagccgcgtt aaagattcac ggtttcaaga taagcgcgcg
      181 ccagcggcaa atcggatttc ataaaatttt ccggcgtgtc gagcaggacg attctgccct
      241 tgtccatcaa acaaattctg gtagccagca caaacgcttc ctgtaaatcg tgcgtgacga
      301 agacggcggt tttgcccaat tctctgacca gtccggcaaa ttccttctgc agattggtgc
      361 gcgttatggc gtcgagcgcg ccgaaaggct cgtccagcaa aagcaattcc ggatttgccg
      421 ccaaagcccg cgccacgccg acgcgctggc gctgcccgcc ggaaagctcg tgcggaaagc
      481 gcgcggcaaa aatttccggc ttcagcccga ccagctcgag catttccgcc gttcgcgcgc
      541 gctttttctc ggcgttccag ttttccaaat ccggcacgag cgcgacattc cgctcaatcg
      601 taaaatgcgg aaaaagcccg gcttcctgca aaacgtagcc ggtttgacgc cgcagtttta
      661 tcgcgtccca atcaatcgtc gattttccgt gcactaaaac ttcgcccgcg gtcggctcat
      721 ataaacgatt tattaatttt aaagtcgtcg ttttgccgca gccggattcg ccgagcagaa
      781 cgagcgtttc gccgcgaaaa atctgcagac tcaaatcggc gagaatccgc gcgccgttaa
      841 tttcaaaact aacgcgcctg aattcgacga ttggctgctg attttccttc attgtttcgg
      901 attaatatta tcatcaggca ataaagaagc gataggtgag aagtgattgc cgtgatgcgg
      961 aagaaacttc ttttgtctta tcaccaatca cttctttttg tatgattaaa gaatgaaaga
     1021 tttcaagccg agcaaagaat ctttgcgcga cgccgtcaac cgcacgaccg aggatttgat
     1081 tgattacaat ctgaaggttt tgttttgcgg catcaatccc ggcatctggt cgggcgcgac
     1141 cggatttcat ttcgccaagc cgggcaaccg cttctggaaa gttcttcatc tggcgggctt
     1201 tacgaatagg ctcctgcatc cgtccgagga acacgagctt ttagcgctcg gctacggcat
     1261 cacgagcctc gtcaagcgca cgaccgcccg cgccgacgag ttagccgtcg aagaattcgt
     1321 cgccggcgga aagcttctag tgaaaaaaat cgacgaatac aagccgcaga atctcgccgt
     1381 tctcggcatc ggcgcgtatc gaaccgcctt taataaaccg aaagcgaaac tcggcttgca
     1441 ggaagataaa atcggcggcg cgcgcgtctg gctgctgccg aatccgagcg gtctgaacgc
     1501 tcattaccag gttgacgact tagccgcgct tttcggcgaa ctgcgaaaaa cggttgaaaa
     1561 ataaaattta tcagcctagc attaaaacat taaagtttta gcacaggaaa cggagaatat
     1621 actgagagct gaagtcgatg taaacggctt gcggctcaac gcgttcgttt cagacgcgga
     1681 ttttgccgga aagctcccga tagttatcgt tcagggcttg ggaatttccg cgagctatat
     1741 gattccggcg atgtcggaga tggcgaaata cgcgaaagtc ttcgcgctcg atttgcccgg
     1801 cttcggcgac agcgaaaagc ctctgcacat ttttaatatc gccgagctgg cggacgtcgt
     1861 cgccgattgg atgagcgcct ccggaatcga acgcgccgtg ctgctcggac attcttttgg
     1921 cagtcagata gcggcggagt ttgcgctgcg gcatccggaa atgatcgagc gaatagtttt
     1981 cgccgcgccg acgtttgacc ggcacgccag aagctttttc cggcaattcg ggcgtttgct
     2041 tttaaattcg cgaaacgagc cgttttcgct gattatgctc gccgtcaaaa gctattttaa
     2101 attcggcatc agaagagaag cgcgaacgct gaagtttgcg ctcgccgacc gcatcgagga
     2161 taagctgccg cggattggca ttccggcgct cgtcgtcaga ggcgaattgg acacggtcgt
     2221 gccgcagggc tgggcggaag aagtagcgga tttattgccg gacggtaaat tagccgtcat
     2281 tgccggcgga acgcacggcg taaattacca atcgccgcga gaatttgccg ccgctattcg
     2341 tgaatttctc gacgattgaa ccggggcgcg aattgagaaa atattaaacg acaagggaag
     2401 aggaaaatta tggcttcgga atgggcggaa gaagcggaca ggcaggcgaa agcgatgacg
     2461 cgcgatttga ttgaggtcgg cagaacaaaa ggcttccgcg tcgattatca aaaacgcgag
     2521 gcgcgcttta tgctgcgtgc gggcacggtt ataacctatt tttccgcgca cgattacgaa
     2581 acgccgaaag gaatggactg gctgcaattg cgcatcgacg ttctcaaaca ggtcagacaa
     2641 gccgcgccgc cggaagagaa atagattatt tatttacttt gttcgtcata acacgtttca
     2701 gatttgatgt ttattcgaca ggcagttaaa cgggaagttg gaaaaacgtt ttaacaaatc
     2761 gaagatgaac ccggactttc cgcagacatc tctgccgacg ccattggcgc aaatgatttc
     2821 cggttataac cggcgtcaaa atcgcaccgg cttttctgcg gcgcgcgttt tccgtctgga
     2881 cgcggaaaat aaagattctc tttatctgaa aatcgctccg cgcactccgg ctcgctcgct
     2941 tttacaggaa aagacaaagc tggaatggct caaaaaccgt cttcccgtgc ccgaagtctt
     3001 gtcgtttgcc gaagatgaaa acgccgatta tcttttgctt tccgccattt ccggcgtcga
     3061 tgcgagcgac gattccctga aaaacgacat tccgcggata atcgagcaat taacggacgg
     3121 tttaaagatg attcacgctt taccgctcga aagctgcccg tttgacgaaa gcctcgatta
     3181 caaaatcgag ctggcggaaa aaatgatgaa agacaatctg gttgacgaaa cggattttga
     3241 cgaaattaat cagggcaaaa ccgccgccga tttgttccgc gagctaatcg aaaacaagcc
     3301 cgccgccgca gattcggttt ttacgcacgg cgattactgc g
//
LOCUS       DASWNE010000403         3059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_225390, whole genome shotgun sequence.
ACCESSION   DASWNE010000403 DASWNE010000000
VERSION     DASWNE010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225390"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3059)
                     /locus_tag="VGB68_08105"
     CDS             complement(<1..>3059)
                     /locus_tag="VGB68_08105"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0049230.1"
                     /translation="SVSELLARPRLTEKLQANLNAPITLVAADAGCGKTTLIADFIRS
                     QSRQTVWYQLDHTDADPFIFLGYISHGIKNFAPAFGETILPYLTEATDELLRFPERAV
                     DLLLNEILSTLEQPLILVLDDYHHIGRETPVHQLVDRLLLYSSDMLHIIITTRDLPPL
                     AIMRRRSQSAALVLTREDLLFNDEEVRQLFRQTLNIELKEAEIAEYRQRTHGWITALQ
                     LVRQVAEREIYSHSNAPAVDLHQILQQSEKDIFDYFAEEVFSREPEDVQNLLLRLSLL
                     ESLPLDACSELFPNMRCSAILPELVQKNVFLTVVGDSKTNEEYRLHPLFRDFLLRRLR
                     SEIGRAEVAEERNRVAAFYLKNNQWEKALPYLLQAENFERAAQIIAAGGGDWLASGAI
                     TTLSIFAEKIPLEFLEKYPRALLHKSEIARLQGETDKSSNLLSRAVKLLHEENDTVGE
                     AEALHALASLARRKGKCGEAFEYLEKAEKLVDESEETYLKCSNTRGLCLVAQGAWTSA
                     EQQFRLALELAEKQGNEHYIRLITHNLALPSGFRGDFGEALRWFKRIFRHDQPDSQLP
                     QEAIGHLNVARLHLYRGEFGDAEPHLERALELCQLYNLKPLRGEILEAYGNFYRDQQD
                     FAHAGEFYERARKAYEEAEVNLATRELDEERAKFYLLRNDAVKARGLLENLLEARRKL
                     GNEILLKAVRLGLCRARLALGETENLAEEIEEILAFYQDQNLYYDEAAASLLLAQLYF
                     AQGERKKMFPPLERALDLTARFDYEHWLGGEIRKNPQMFADEEITEKLPLDLRPASQE
                     PRVESQEPDKISISTPDARLATPEIVDLTVKMLGFVEIYRDKSKPFAADAWTTRRARD
                     IFCYIASSRHRRVEKDVLIDTFWSEEDLASIEKNFHPTISHIRKALNSRQALKQNFLV
                     FRDGAYQLNPELSYSIDTEDFDRFMAEAEKAKRDGEDQAFRQNLDAAHALYRGEFMAG
                     IYEPWAEEQRGYYREQYARILNALAKSAFKEKNWSQSVKLAQEILQD"
BASE COUNT          664 a          855 c          838 g          702 t
ORIGIN      
        1 tcgtcctgca aaatttcctg cgccagcttt acgctctgcg accagttttt ctctttgaaa
       61 gccgatttcg ccagcgcgtt cagaatgcgc gcgtattgtt cgcggtaata gccgcgttgc
      121 tcttccgccc agggctcgta aatgccagcc atgaattcgc ctcgataaag cgcgtgcgcc
      181 gcgtccagat tttggcgaaa agcctggtcc tcgccgtcgc gtttcgcctt ttcggcttcc
      241 gccataaagc ggtcgaaatc ttccgtgtca atcgaatacg agagttccgg attaagctgg
      301 taagcgccgt cgcgaaaaac gagaaaattt tgtttgagcg cctgtctgga gttcagagct
      361 ttgcgaatgt gcgaaatcgt cgggtgaaaa ttcttttcaa tcgacgccaa atcctcttcg
      421 ctccagaacg tgtcaatcaa aacatctttt tcgacgcgcc ggtggcggct cgaagcgata
      481 taacaaaaaa tgtcgcgcgc gcgtctggtc gtccaggcgt ccgccgcgaa aggcttcgat
      541 ttatcacggt aaatctcgac aaaaccgagc attttgacgg tcaaatcgac gatttcagga
      601 gtcgcgagcc gggcgtccgg agtcgagatg gaaattttgt ccggttcctg actctcgact
      661 ctcggctcct gactggctgg gcgtaaatcg agcggcagtt tctcggtgat ttcctcgtcg
      721 gcaaacatct gcggattttt tcgaatctcg ccgccgagcc agtgttcata atcgaaacgc
      781 gccgttaaat ccagagccct ctcaagcggc ggaaacattt ttttgcgctc gccctgcgcg
      841 aaataaagct gcgccagcaa aagggacgcc gccgcctcgt cataataaag attctggtct
      901 tgataaaacg cgaggatttc ttcgatttct tcggctaaat tttcggtctc gcccagcgcc
      961 agccgcgcgc ggcacaaacc caggcgaacg gctttgagca gaatttcgtt gcccagcttc
     1021 cggcgcgctt ccagcaaatt ctccaaaagt ccgcgagctt tcaccgcatc attgcgcagc
     1081 agataaaatt tcgcgcgttc ttcgtccagc tcgcgcgtcg ccagatttac ttcggcttcc
     1141 tcataagctt tccgcgcgcg ctcgtaaaac tctccggcgt gcgcgaaatc ctgctggtcg
     1201 cgatagaaat taccgtaagc ttctaaaatt tcgccgcgca gaggttttag attataaagc
     1261 tggcaaagct ccagcgcgcg ctccagatgc ggctcggcat cgccgaattc gccgcggtaa
     1321 agatgcagcc gcgcgacgtt taaatgcccg atagcttcct gcggcagctg cgagtcgggc
     1381 tggtcgtgtc gaaaaatcct tttgaaccag cgcagagcct cgccaaaatc gccgcgaaag
     1441 ccggaaggca aagccagatt gtgtgtaatc aggcgaatat aatgctcgtt gccttgcttt
     1501 tcggcgagct ccagagccag gcgaaattgc tgctcggcgc ttgtccaggc gccctgcgcg
     1561 accagacaaa gcccgcgcgt gttcgagcat ttcagataag tttcctcgct ctcgtcaacc
     1621 agtttttcgg ctttttccag atattcgaac gcctcgccgc atttgccttt gcgccgcgcc
     1681 agactcgcca gcgcgtgcag ggcttcggct tcgccgaccg tgtcgttttc ttcgtgcaga
     1741 agcttgacgg cgcggctcaa aagattggaa gatttgtcgg tctcgccctg caggcgggcg
     1801 atttccgatt tatgcagcag agcgcgcgga tatttttcca gaaattcgag cgggattttt
     1861 tcggcgaaaa tgctcaaggt cgtaatcgcg ccgctcgcca gccagtcgcc gccgcccgcc
     1921 gcgataatct gcgccgcgcg ctcgaaattt tccgcctgca aaagatacgg cagagccttt
     1981 tcccattgat tgtttttcag gtaaaacgcg gcgacgcgat ttctttcttc ggcgacttcg
     2041 gcgcgaccga tttccgagcg caggcggcgc aggagaaaat cgcggaaaag cgggtgcagc
     2101 cggtattcct cgtttgtctt gctgtcgccg acgaccgtca aaaaaacgtt tttctgcacc
     2161 agctcgggca gaatggcgga acagcgcata ttcggaaaaa gctcgctaca ggcgtctagc
     2221 ggcagagatt cgagcagcga caggcgaagc agaagattct ggacgtcttc cggctcgcgc
     2281 gagaaaactt cttcggcgaa ataatcaaaa atatcttttt ccgactgctg caaaatctgg
     2341 tgcaaatcga cggcgggcgc gttcgaatgc gaataaattt cgcgctcggc gacctgacgg
     2401 acaagctgca gagccgtaat ccagccgtgc gtccgctggc gatattcggc aatttccgct
     2461 tcttttaatt cgatattcag cgtttgccgg aaaagctggc gcacttcttc gtcgttaaaa
     2521 agaaggtctt cgcgcgttaa gaccagagcg gcggattgcg agcggcggcg cataatcgcc
     2581 agcggcggca aatcgcgcgt cgtgataata atgtgcagca tatcggacga gtacaataaa
     2641 agccggtcaa ccagctgatg caccggcgtt tcgcgcccga tgtggtgata atcgtccaaa
     2701 actaaaatca acggttgttc cagagtgctg agaatctcgt tcagaagcaa atcgacggcg
     2761 cgttccggaa agcgcagcag ctcgtccgtc gcttccgtca ggtaaggcag aatcgtctcg
     2821 ccgaaagcgg gcgcgaaatt tttgatgccg tgcgagatgt aaccgagaaa aataaacggg
     2881 tcggcgtccg tgtggtcgag ctgataccag acggtctggc gcgactggct gcgaataaaa
     2941 tcggcgatga gcgtcgtttt gccgcagccg gcgtcggcgg cgaccagcgt tatcggcgcg
     3001 ttgagattcg cctgcaattt ttccgtcagg cgcgggcgcg ccagcagttc cgaaaccga
//
LOCUS       DASWNE010000404         1946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_190146, whole genome shotgun sequence.
ACCESSION   DASWNE010000404 DASWNE010000000
VERSION     DASWNE010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190146"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..555
                     /locus_tag="VGB68_08110"
     CDS             52..555
                     /locus_tag="VGB68_08110"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEY0049231.1"
                     /translation="MFCPNCGANNSTEQKFCRACGLNLEKTAESLVEQIPSAESANLL
                     RREKLIERFGNFALGGLGVVILLAVTILIYTIVEKLLVGGTNVYFAVLLTAFIVFAFL
                     SLIFVFFNENLKEKKAKTNPALDNELPERKQTAKLPEEKPFEPVPSITENTTGLLYAE
                     QKTKKFE"
     gene            552..1124
                     /locus_tag="VGB68_08115"
     CDS             552..1124
                     /locus_tag="VGB68_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049232.1"
                     /translation="MSLLDVQNLLARIFTDKDLRLRFLLEPEKTGAENNLNEAEIEQI
                     KQILPEQINFFADSLFYKRLHEVEKFLPLTRKVLGKDFEKYFREFANQFLPQTIKKHL
                     EDAIEFGGFLQLKEIKPFWTKDLAKFEQARLAFNNSEKRFIFKRFDFDIREIIKGISG
                     EDARAQTDFPKRKTYGVWLRLGARTKHFIY"
     gene            1190..1711
                     /gene="def"
                     /locus_tag="VGB68_08120"
     CDS             1190..1711
                     /gene="def"
                     /locus_tag="VGB68_08120"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY0049233.1"
                     /translation="MALLKIVQYPEPVLLTVGKPVGEEEFDEDLRRLVKDMFETMYAA
                     GGVGLAAPQVAVSKRLFVMDCSGGEDPAQKIALINPEIIAQEGEQTGDEGCLSFPGIY
                     TQVKREVRTVVRYQDVFGNVRELDGTDLTARCVLHETDHCDGIVFLDRMTMLKREMAK
                     RKIRKLQKTGEWE"
     gene            1715..1912
                     /locus_tag="VGB68_08125"
     CDS             1715..1912
                     /locus_tag="VGB68_08125"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEY0049234.1"
                     /translation="MPPKIRELIAELERNGFTNRGGKESHRNYEHILGIRVTISGKLG
                     DDARKYQEQLVKDVLRKVKAK"
BASE COUNT          575 a          403 c          489 g          479 t
ORIGIN      
        1 tcaaccgagc ttttgtttgc cgaaaacaaa acgagaaaat tggggtaaat tatgttttgt
       61 ccgaattgcg gagcaaacaa ttcaaccgaa caaaaattct gtcgcgcgtg cggcttgaat
      121 ctggagaaaa ccgccgaatc gctcgtcgag cagattcctt cagccgaaag cgcgaatttg
      181 ctgcgccgcg aaaagttaat cgaaagattc ggcaatttcg cgctcggcgg tctgggcgtg
      241 gttattttat tagccgtgac gattttgata tacacaatcg tcgaaaaact tctggtcggc
      301 ggaacgaacg tctatttcgc cgtcctgctg acagcgttta tcgtcttcgc ctttttgtct
      361 ttgatttttg tgttttttaa cgaaaatctg aaagaaaaaa aggcgaagac aaatccggcg
      421 ctcgacaatg aattgccgga aagaaagcag acggcgaagc taccggaaga aaagcctttc
      481 gagccggttc cgagcatcac ggaaaacacg accggcttgc tttacgccga acaaaagact
      541 aaaaaatttg aatgagcctt ctcgacgtgc aaaaccttct ggcgcgaatc tttaccgaca
      601 aagatttgcg tcttcgattt ttgctggagc cggaaaaaac cggcgcggaa aataatttga
      661 acgaagcgga aatcgagcaa atcaaacaaa tcctgcccga acagattaat tttttcgccg
      721 attcgctttt ttacaaacgc ctgcacgaag tcgaaaagtt tctgcctttg acgcggaaag
      781 ttttaggcaa agattttgaa aaatatttcc gcgagtttgc caaccaattt ctgccgcaga
      841 caatcaaaaa acatctcgaa gacgccatcg aattcggcgg atttttgcaa ttaaaggaaa
      901 ttaaaccttt ctggacgaaa gatttagcga aattcgagca ggcgcgattg gcgtttaata
      961 attcggaaaa acgatttata tttaagcgtt tcgacttcga tatacgcgag attataaagg
     1021 gaatctccgg cgaagacgca agggcgcaaa ccgattttcc gaaacggaaa acctacggcg
     1081 tctggttgag attaggcgcg cggacgaaac attttattta ctgagtttta ccttctactt
     1141 ttaactttta acttttgtct tttggctttt gccttataaa atagaattta tggctttact
     1201 caaaattgtt cagtatcccg aacccgtttt gctgacggtc gggaaacccg tcggcgaaga
     1261 agaattcgac gaagatttgc gccgtctggt caaggatatg ttcgagacga tgtacgcggc
     1321 gggcggcgtc ggcttggcag cgccgcaagt tgccgtttca aaacgtcttt tcgtgatgga
     1381 ttgctcgggc ggcgaagacc cggcgcagaa aatcgctttg ataaacccgg aaatcatcgc
     1441 gcaggaaggc gagcagaccg gcgacgaagg ctgtctttcg tttccgggaa tttacacgca
     1501 ggtcaaacgc gaagtgcgaa ccgtcgtgcg ttatcaggac gttttcggaa acgtgcggga
     1561 gttggacggc acggatttaa cggcgcgctg cgttctgcac gagacggacc attgcgacgg
     1621 aatcgttttt ctggacagaa tgacgatgct gaaaagagaa atggcgaagc gaaagattag
     1681 aaaattgcag aaaaccggcg aatgggaata atcgatgccg ccgaagatta gagaattgat
     1741 tgccgagctt gaacgcaatg gttttacgaa tcgcggcgga aaggaaagtc atcgaaatta
     1801 tgagcatatt ttaggaataa gagttacaat ttccgggaaa ctcggcgatg acgccagaaa
     1861 atatcaggaa caattagtta aagacgtatt aagaaaggta aaggcaaaat gaagagtcca
     1921 gcagacgttt atgagaaaat tgttta
//
LOCUS       DASWNE010000405         1916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_154572, whole genome shotgun sequence.
ACCESSION   DASWNE010000405 DASWNE010000000
VERSION     DASWNE010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154572"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..560
                     /locus_tag="VGB68_08130"
     CDS             168..560
                     /locus_tag="VGB68_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049235.1"
                     /translation="MRKHIRILGFIYTGIAVHLGLATLLFIAFALFGKNAPPGGLTVI
                     IIFAGLTLWFLQTGLGLWKLRRGARLMALIVAFVFMLVLNGALLFADGGNFARSTGQK
                     IFHLSCIAIGIYTVGVLLFPGSSDSFQE"
     gene            662..1291
                     /locus_tag="VGB68_08135"
     CDS             662..1291
                     /locus_tag="VGB68_08135"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEY0049236.1"
                     /translation="MGEWFPVLSLALGSAWTSGINLYATVTVLGLLQRFGATRLPGGL
                     EVLDNWWIIGIAGFLYLVEFVADKVPYVDSVWDVIHTFIRVPAGAIVAYAATNSLDPS
                     VSVIAGLLGGGLAFSSHGTKAALRATANLSPEPVSNWALSFIEDGIAIIGAFLAVFAP
                     VIIAVVLVVFLIIFFWLMPKFLRAMRKMANGTRSFFTGKGFRDVSRGTG"
     gene            1292..1441
                     /locus_tag="VGB68_08140"
     CDS             1292..1441
                     /locus_tag="VGB68_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049237.1"
                     /translation="MNPSNHVCERDFAVFGGGCFAARARYKIFLKAAAQTEASEIARR
                     EIKNE"
     gene            1444..1674
                     /locus_tag="VGB68_08145"
     CDS             1444..1674
                     /locus_tag="VGB68_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049238.1"
                     /translation="MERLFQILAVILAGVAAFFFWRGNWDAGFVSAVFGAVSFFLSIR
                     FQVRERLKIRDAEREADRQREESAAEEAMNDE"
     gene            1730..>1916
                     /locus_tag="VGB68_08150"
     CDS             1730..>1916
                     /locus_tag="VGB68_08150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0049239.1"
                     /translation="MAKIVLATFGSLGDLHPMLALAIELQRRGHEIVINTLEVYREKI
                     DALGFEFFPMRPDLNPED"
BASE COUNT          430 a          432 c          537 g          517 t
ORIGIN      
        1 acgtgaaaac gagcctccgc gaaatgaaag gaacggttaa caaacccgaa gtattcgctt
       61 tcaaagacgc taaagtcacg atttatgaaa acagcggcta caacaaaata attatcgaaa
      121 gataacgcgc aggattcgag cgatcgtttt tcttcagaag aaaatttatg agaaaacaca
      181 tcaggattct cggctttatt tatacgggaa ttgccgtgca tttaggattg gcgacgcttc
      241 tttttatcgc ctttgcgctg tttgggaaaa acgctccgcc gggcggtttg acggttatca
      301 ttatttttgc cggtctgacg ctgtggtttt tgcagaccgg actgggactt tggaaactgc
      361 ggcgcggcgc gcgcctgatg gctctgattg tcgctttcgt gtttatgctt gtcttaaacg
      421 gcgcgctgtt atttgccgac ggcggtaatt ttgcgcgctc gaccggacag aaaatttttc
      481 acctctcctg catcgccatc ggcatataca cggtcggcgt tttacttttt cccggctcaa
      541 gcgattcttt tcaggaataa attcgagcga aaaacattga aaggaaaatg ataagctaga
      601 gagaatatcg gcatcaaata aatgtcaaag ctcggatata tttttatcaa ggagaaaaac
      661 gatgggcgaa tggtttccgg ttttgagtct ggcgctcggt tcggcgtgga cttcgggcat
      721 taatctctac gcgacggtga cggtgctcgg gcttttgcag cgattcggcg cgacgcggct
      781 gcccggcggg ctggaagtgc tcgacaactg gtggattatc ggcatcgccg gatttttgta
      841 tctggtcgaa tttgtcgccg acaaagttcc ttatgtcgat agcgtctggg acgtaattca
      901 cacgtttatt cgcgttccgg cgggcgcgat tgtcgcctac gcggcgacga atagtctcga
      961 cccgagcgtt tcggtcattg ccggacttct cggcggcggc ttggcttttt cttcacacgg
     1021 aacgaaagcg gcgctgcgcg cgacggcgaa tctttcgccc gagccggttt ccaactgggc
     1081 tttgtcgttt atcgaagacg gaattgcgat aatcggcgcg tttctcgcgg tttttgcgcc
     1141 ggttatcatt gccgtcgtgc tcgtcgtttt tctaataatc ttcttctggc tgatgccgaa
     1201 atttttgcgc gcgatgagga aaatggcgaa cgggacgcgt tcgtttttta cgggcaaagg
     1261 ttttcgggac gtctcgcgcg gaaccggata aatgaatccg tccaatcacg tttgtgagcg
     1321 cgattttgcc gttttcggcg gcggttgttt tgccgctcgc gctcgttata agatatttct
     1381 taaagcggct gcgcagacgg aagcgagcga gattgcgcgc cgggaaatta aaaacgagta
     1441 acgatggaaa gactttttca aatattagcg gtgattctag ccggagtcgc cgcgtttttt
     1501 ttctggcgcg gaaactggga cgccggtttc gtctcggcgg ttttcggcgc ggtcagcttt
     1561 tttctgagca ttcggtttca ggtcagggaa agattgaaaa tcagagatgc ggaaagggaa
     1621 gccgacaggc agagggaaga atcagccgca gaagaggcga tgaatgatga ataatgaacg
     1681 cggaacaagc gatttatcct tcgtcattta tccatcatcg ctttagaata tggcaaaaat
     1741 agttttagca accttcggct cgctcggcga tttgcatccg atgctggcgc tggcaatcga
     1801 gctgcaaagg cgcgggcacg agattgtcat taatactctg gaggtttacc gcgaaaaaat
     1861 cgacgcgctc ggctttgagt ttttcccgat gcgtcctgat ctgaatcccg aggatg
//
LOCUS       DASWNE010000406         3947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_261447, whole genome shotgun sequence.
ACCESSION   DASWNE010000406 DASWNE010000000
VERSION     DASWNE010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261447"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /gene="dnaG"
                     /locus_tag="VGB68_08155"
     CDS             complement(<1..1106)
                     /gene="dnaG"
                     /locus_tag="VGB68_08155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEY0049240.1"
                     /translation="MRFEESFIDELRQRADLVRIIEPYAPLKKKGANWMGCCPFHQEK
                     TPSFSVNPAKGFYKCFGCGKGGNAFSFVMEIEGLTFPEAIKKVAEVSGVPLPAPVDDK
                     KFEHVKKKKEAEKKIADAVIELNQFALELWESQLQETSGKQAREYLEARGISEETCKI
                     FRIGYAPDSWDALMSFLKSKGADEKLIEQSGLVSINEEKKRVYDRFRGRVMFPVLDVN
                     GKPIAFGARILDKGEPKYLNSPETPAYIKGEHLYGLFQDKEDIRRKKFAILVEGYLDL
                     IALYQAGVHNAVASLGTAFTPNQAKLLGRFARKIVVNYDGDSAGVKAARRAIETLLAE
                     DFEIKVLVLPNGQDPDDFIRANDVEAYNIQRGNA"
     gene            complement(1131..2099)
                     /locus_tag="VGB68_08160"
     CDS             complement(1131..2099)
                     /locus_tag="VGB68_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049241.1"
                     /translation="MKSPKIFLALLILFAFVHICFGQNSEETQPAENWLWTEYKIFPD
                     SRLYHLYERYSKEDIVKFREKLDLLKNGEFQDEWEGIYSVAYEETVGFSQFRWRRDVG
                     FVSFGIYTCLPELRSVNYGKVVDTPDFIQILPEYAENSPRRQTETKYVKVKWGEHRFL
                     VEESSLSAFAEKAVGIFVNPENPDDENYHKWVNQWFKGDYEKPYVGLPEFPDKYKKFQ
                     RSPIETKIITVGKRFVEENSASYNVTIGAGKNKGIGKGMIFDVPEIENSIEITRVGDK
                     TAVGIIVRSVDENNNDECFGDDFNKVVCAKIKSGLKVKTQIGVFSW"
     gene            complement(2161..3099)
                     /locus_tag="VGB68_08165"
     CDS             complement(2161..3099)
                     /locus_tag="VGB68_08165"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEY0049242.1"
                     /translation="MHSYRIFYSPYYYADIGEGHVFPIKKFELVRDRLLGEGTLRKEE
                     ISEPQPAAIDDVRLVHTEDYVTRLIEGELTAKEIRRLGLPWSKSLVRRSFLACSGTIS
                     AARHALSNKVASNLAGGTHHAFPDRGEGFCVLNDVAVAIRVLQKEKLAAKFLIVDCDV
                     HQGNGTAFIFQNSPEAFTFSMHGAKNYPLHKEQSTLDLELPDGTGDAEFLEILHEALP
                     RIFLHNPDIVFYLGGADPFEKDKLGRLKLTRDGLRRRDEMVLQFAAEREIPIVTTMSG
                     GYAADINDTVEIHCNTIRAAKKIFFAEDKVLARNVN"
     gene            complement(3342..3665)
                     /locus_tag="VGB68_08170"
     CDS             complement(3342..3665)
                     /locus_tag="VGB68_08170"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEY0049243.1"
                     /translation="MFIPAKNARTRPQSAKREKANSWLGFAVVVALSSMICLTVNLRA
                     YSELRAEIDQHQILNTEVNQLTTENSALQEEIQQIKTDKTMIEREARRLGMSRPNEKV
                     LVPAN"
     gene            complement(3676..>3947)
                     /locus_tag="VGB68_08175"
     CDS             complement(3676..>3947)
                     /locus_tag="VGB68_08175"
                     /inference="COORDINATES: protein motif:HMM:NF012626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049244.1"
                     /translation="LEAGRIGVKLTPEIAEKLKEKLAQEPTEIGNRRIEKINRMDGVK
                     FIFADGSWMLMRPSGTEPLVRIYAEADTQSDLEVLLEQGRNYLLG"
BASE COUNT          932 a          982 c          921 g         1112 t
ORIGIN      
        1 aaagcgttgc ctctttgaat gttatatgct tcgacgtcgt ttgcgcggat aaaatcgtca
       61 gggtcctgcc cgtttggtaa aactaaaact ttaatctcga agtcttccgc caacagagtt
      121 tcgatagcac gtctcgccgc tttcacgcca gcactatctc catcatagtt cacaactatt
      181 ttacgcgcaa atctgcccaa tagtttcgct tgattgggag tgaaagccgt gccgagactg
      241 gcgaccgcgt tatgcactcc ggcttgataa agcgcgatta aatccagata gccttcgacc
      301 agaatcgcga actttttgcg ccggatgtct tctttatcct gaaacagacc gtaaaggtgc
      361 tcgcccttga tataagccgg agtttcgggc gaattcagat atttcggctc gcctttatcg
      421 agaattcgcg cgccgaaggc aatcggtttt ccgttaacgt cgagaaccgg aaacatcacg
      481 cgtccgcgaa aacggtcgta gacgcgtttt ttttcctcgt tgatgcttac cagaccgctc
      541 tgctcgatta atttttcgtc cgcgcctttc gatttcagaa aagacatcag cgcgtcccag
      601 ctgtcgggcg cgtagccgat gcgaaaaatt ttgcacgtct cttccgagat gccgcgcgct
      661 tccagatatt cgcgcgcctg ttttccgctt gtttcctgca gctgactttc ccagagttcg
      721 agcgcgaatt gattcagctc gataacggcg tcggcgattt tcttttcggc ttcttttttc
      781 tttttgacgt gctcgaattt tttatcgtca accggcgcgg gcagtggaac gcccgacact
      841 tccgccactt tttttatcgc ttcgggaaac gtcagacctt cgatttccat tacgaaacta
      901 aaggcattac cgccttttcc gcatccgaaa catttgtaaa aacctttcgc cggattcacc
      961 gaaaacgaag gcgttttttc ctggtgaaac gggcagcagc ccatccagtt ggcgcctttt
     1021 ttcttgagcg gcgcgtacgg ctcgataatc cgcacgaggt cggcgcgctg gcgtagttcg
     1081 tcgatgaaag attcttcaaa acgcatttaa ttttaaatta ataacgaagt ttaccagcta
     1141 aacacgccga tttgagtttt gacctttaaa cccgatttta ttttcgcgca gacgacttta
     1201 ttaaaatcat cgccaaagca ttcatcgttg ttgttttcat caacgcttct gacgattatc
     1261 ccgacggcag ttttgtcgcc gactcgtgtg atttcgatag agttttcaat ttcgggaacg
     1321 tcgaaaatca tacctttccc gatgccttta tttttgcccg cgccaattgt gacgttatac
     1381 gaagcgctgt tttcttcaac gaatcgtttt cctacagtaa taattttggt ttcaattggc
     1441 gaacgctgaa actttttata tttatcggga aattcgggca aaccgacata tggtttctcg
     1501 taatcgcctt taaaccattg atttacccat ttatgataat tttcatcgtc ggggttttcg
     1561 ggattgacaa atattccgac cgctttttcc gcgaaagccg aaagcgaaga ttcttcgact
     1621 aaaaaacgat gctcgcccca ttttactttg acgtacttag tttcggtttg cctgcgcgga
     1681 gaattttccg cgtattcggg caaaatttga ataaaatccg gtgtatcaac gactttgccg
     1741 taattgaccg agcgaagttc gggcaaacag gtgtagatgc cgaaacttac aaagccgaca
     1801 tcccgacgcc atcgaaactg cgaaaatccg actgtctcct cgtatgcgac tgaatatatt
     1861 ccttcccatt cgtcctgaaa ttcgccgttt ttcaacaaat cgagtttttc tctgaatttt
     1921 acgatgtctt ccttcgagta tcgttcatag agatgataaa gacgcgaatc gggaaatatt
     1981 ttatattccg tccacaacca attttcggca ggttgtgttt cctcggaatt ttgcccgaaa
     2041 caaatatgaa caaacgcgaa taaaatcagc aaagcgagaa agattttcgg cgatttcata
     2101 acttttcctt cggcggtttt gtctctgacg cattatagcg caaacgcgcg ttttagatta
     2161 ctaatttacg tttcgcgcca aaactttatc ttcggcgaaa aatatttttt tcgcggcgcg
     2221 aatcgtgttg cagtgaatct cgaccgtgtc gtttatatcc gccgcgtagc cgccgctcat
     2281 cgtcgtcacg attggaattt cgcgctcggc ggcaaactgc aaaaccatct catcgcgccg
     2341 gcgcaagccg tctctggtca gcttcaaacg tccgagcttg tctttttcga acgggtcggc
     2401 gccgccgaga taaaaaacga tgtccggatt gtgcagaaaa atgcgcggta gcgcttcgtg
     2461 cagaatttcg agaaattcgg cgtcgcccgt tccgtcggga agctccaggt cgagcgtcga
     2521 ttgctccttg tgaagcgggt aatttttcgc gccgtgcatc gaaaaagtga aagcttcggg
     2581 cgagttttga aagataaacg ccgttccgtt tccttgatga acgtcgcagt cgacgattaa
     2641 aaactttgca gccaattttt ctttttgtaa aactctgatg gcgacggcga cgtcgtttaa
     2701 aacgcagaaa ccttcgccgc ggtcgggaaa agcgtgatga gttccgcccg cgagatttga
     2761 cgcgactttg tttgagaggg cgtgacgcgc ggcgctgatg gtgccggaac aagccagaaa
     2821 agaacggcgc acgagcgatt ttgaccacgg caatccaagc cggcgaattt ccttcgcggt
     2881 caattcgcct tcaatcaaac gcgtcacgta atcttcggtg tgaacgagcc gaacatcgtc
     2941 gattgcggcg ggctgcggct cgctgatttc ctcttttcgc agagttcctt cgccgagcag
     3001 acggtctctg accaattcaa attttttaat cgggaagacg tgtccttcgc cgatgtcggc
     3061 gtaataatac ggcgaataga aaattcggta agagtgcatt ggaaaatttt aacgcgaagc
     3121 cgcaaagaca caaagacaca aggaaaaaaa taatttcctt atccagtcgc aaatctaaaa
     3181 tctaaaatta tctttcttat ggaaggtggt gcggctcgtg ttcgggaaac acgagccgcg
     3241 aaattggcgg aagggcaaac tgttggcagc gcggagaaga taggcaaaag gcagttgttt
     3301 tagggcaggt agtgtgctga cgaattaaac actccgcgaa tttaattcgc cggcacgaga
     3361 actttttcat tcgggcgact cattcccaga cggcgcgctt cgcgctcgat catggttttg
     3421 tccgtcttaa tctgttgaat ctcttcttgc agagccgaat tttcggttgt caattgatta
     3481 acttcggtgt tcagaatttg atgctggtcg atttcggctc tcagctcgct gtaagcgcgc
     3541 aggtttacgg ttaaacaaat catcgaactc agggcgacaa ccacggcaaa gccgagccac
     3601 gagtttgcct tctcgcgttt tgcggattgt ggacgcgtcc gcgcgttttt tgctggaata
     3661 aacactgcgt tcgaattatc ccaataagta gttgcgacct tgctcaagca gcacctccaa
     3721 atcactctga gtatcagctt cggcgtaaat gcggacaagc ggttcggtcc cggaaggacg
     3781 catcagcatc cagctcccgt cggcgaagat aaatttcacg ccgtccatcc gatttatctt
     3841 ttcaattcgg cgattgccga tttcggtcgg ctcctgcgcc agtttttctt tcagcttttc
     3901 ggcaatttcc ggagttaact ttactccgat tctgcccgct tcgagtc
//
LOCUS       DASWNE010000407         6222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_261468, whole genome shotgun sequence.
ACCESSION   DASWNE010000407 DASWNE010000000
VERSION     DASWNE010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6222
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261468"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1716)
                     /locus_tag="VGB68_08180"
     CDS             complement(<1..1716)
                     /locus_tag="VGB68_08180"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY0049245.1"
                     /translation="MKAKFFYLAIFGSMIVSFLMFWQMPAQMQSSRTNNPIDSQKTNE
                     RTAVQDSTTKFDELIENAGRSGSVRVIIGLRADFQIEGKLSLTAQNRQRTAIRQSQNN
                     LLADLSGYNVAGVKRFDFIPFLAAEVDAAALEKIKASPIIVSIQKDRLVQPSLTESTA
                     VVGAAGGMANGFSGSGQAIAILDTGVDKNHSYFAGGKVVSEACYSTTTGTNPDFDSSQ
                     SLCPGGAAESTAPDSGLHCDTAQIGVCWHGTHVAGIAAGRDTTRGHFGVAKDASIIAI
                     QVFSRFNNPDNCGGGAPCISAYTSDIIKGLERVYALRATFNIASVNMSFGEGNFAGYC
                     DGEEPALTAAVNNLRSVNIASVAASGNNGLTSGINFPACISPVVSVGSSNDGGNTTTQ
                     DTVNNFSNSAGFLNLYAPGFLITSAAPNNQLRSEGGTSMAAPHVAGAWAILKQQNSAA
                     TVSEVLTALTSTGRIIIDQRAATGNRRTPRIRVDSASQQFAGTNCVRVPIATNQFINA
                     ALDTGDCAAEAGVNRDLYAFSGAAGGSVAILMTSSEFDTFLYLLDPNGRIIAFDDNSG
                     GGTNAR"
     gene            complement(2047..2751)
                     /locus_tag="VGB68_08185"
     CDS             complement(2047..2751)
                     /locus_tag="VGB68_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049246.1"
                     /translation="MLENLPGYVSVTFILTTFLTVGIFLYAIKRGAFTSTATKFLSFL
                     IPFWLIFQATLGIGGFYLNTGIAPPRLMLFALFPALAAIILLFILARKSLIEQLPLQT
                     LTYLHVVRIPVELVLLWLYQGGQVPQLMTFEGRNFDILAGITAPVMAWLAFRGGKINR
                     PLLIVWNTLALGLLLNIVVNAVLSFPFPLQQFAFDQPNRGVLYFPFIWLPSTIVPIVL
                     FCHLASLWQLARNNRL"
     gene            complement(2897..3628)
                     /locus_tag="VGB68_08190"
     CDS             complement(2897..3628)
                     /locus_tag="VGB68_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0049247.1"
                     /translation="MATEIQTNGGGEITTPGALISMRNIWRTYQMGSEELHALRDVSF
                     DVAKGEYLAIIGPSGSGKSTLMNLIGCLDSPSQGSYWINGHLVSAMTDDELARIRNKE
                     IGFVFQTFNLLPRATALHNVELPLIYAGIGASQRHERAQSALASVELGDRVMHRPNEL
                     SGGQRQRVAIARALVNHPSILLADEPTGALDSKTSVEIMALFEKLHADGNTIIVVTHE
                     HEVAERAHRVLSIRDGQIESDERIK"
     gene            complement(3737..5362)
                     /locus_tag="VGB68_08195"
     CDS             complement(3737..5362)
                     /locus_tag="VGB68_08195"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0049248.1"
                     /translation="MALSRKKKIIIGSVAVVLLGIIIIGSIFATRKDTPEVTVVKIET
                     RRELRSTVTASGEVRPIQFINLTSEVQGRIEEIYVKEGDQVTKGQPLVRLDPNQLQSS
                     TDAQLASVQAAQTETQVSRSQVVAAQNQLSQAQQGLNASQAQIDTALQQVATARQQVV
                     NATQQVDTAQTDVDRARKDLETANRELKRTTQLLEAQVASRLQYDADKDKVDQAQISL
                     RNALARLQSQRNAVTIAEKQVNEAQARVNEARARANQQSVAVKDARRGVDTASYSADA
                     TSKRAEQQAALLRGTRSQRDKSMQTAPINGVIADIPSKVGTFAVANLSSTPLLTIADM
                     STINVEVKVDETEIDKVEVGQTAKIKVDAFGEKEILGEVTQKTPLALGKSQTSGGLST
                     NINVQEAKEFRVVIQLKDVADAVREGLRPGMSATATITTRTVENVVTVPLQAIIEKQP
                     EAAPSPTVQGDAPASNEKPKPIKGVYVMDGNKVKFVEVTTGITGESDIQITSGLSEGQ
                     EVITGPSRVLKTLKENDLVKRQTKKEGDNANKS"
     gene            5665..>6222
                     /locus_tag="VGB68_08200"
     CDS             5665..>6222
                     /locus_tag="VGB68_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0049249.1"
                     /translation="MIKCPTCGTENLEGSQFCDECGAALSGARQRNDSDSVLTAYPST
                     PNFQSANVTSVGVPTVVEQLKVEEDTEVKAVSQTDDGLSKGVHAVLIIERGASVGTEF
                     GLTSEESNIGRWDADNGIFPDVDLDTHDPEAKVSRRHARIIQENGRYMLEDLGSTNGT
                     FINRGRRLIPGNRQPLNDGDEVIVGK"
BASE COUNT         1387 a         1624 c         1743 g         1468 t
ORIGIN      
        1 gcgcgcgttc gttccgccgc cgctgttatc gtcaaaagcg ataatgcgcc cgttcgggtc
       61 gagcagatac aaaaacgtat cgaattccga cgaagtcatc aaaatcgcca ccgagccgcc
      121 cgccgcgccg ctaaaggcgt aaaggtctcg gttgactccg gcttccgccg cgcaatcgcc
      181 cgtgtcgagc gcggcgttga taaactggtt ggtcgcaatc ggcacgcgga cgcagttcgt
      241 tccggcaaac tgctgcgacg ccgaatcgac gcggatgcgc ggcgtccggc ggtttccggt
      301 ggcggcgcgc tggtcgatga tgattctgcc cgtgctggtc aaagcggtca ggacttcaga
      361 aacagtcgcc gcagaatttt gctgtttgag aatcgcccac gcgcccgcga cgtgcggcgc
      421 tgccatcgaa gtgccgcctt cgcttcggag ctgattgttc ggcgctgccg aggtaatcag
      481 gaatccgggc gcgtataaat ttaaaaagcc ggcgctgttg gaaaaattgt tgaccgtatc
      541 ctgggttgtg gtattgccgc cgtcattgct cgagccgacg ctgacgaccg gcgagatgca
      601 ggcgggaaaa ttgatgccgc tggtcaaacc gttgttgccc gaagcggcga cgctggcgat
      661 attgaccgag cgcaggttat tgacggcggc tgtcagcgcc ggttcttcgc cgtcgcagta
      721 accggcgaaa ttgccctcgc cgaagctcat attgaccgag gcgatattaa aggtcgcgcg
      781 cagagcgtaa acgcgctcca agcctttgat aatatcggag gtgtaggcgc tgatgcacgg
      841 cgcgccgccg ccgcagttgt cgggattgtt aaatctcgaa aaaacctgaa tggcgataat
      901 cgaagcgtct ttcgccacgc cgaaatgccc gcgcgtcgtg tcgcgtccgg cggcgattcc
      961 ggcgacgtgc gtgccgtgcc agcagacgcc gatttgcgcg gtgtcgcaat gcagtcccga
     1021 atcgggcgcg gtcgattccg ccgcgccgcc cggacacaaa ctctgcgagc tgtcaaaatc
     1081 gggatttgtt cccgtcgtcg tcgagtagca cgcctcggac acgactttgc cgcccgcgaa
     1141 ataactgtga tttttgtcga cgccggtgtc gagaatggcg attgcctgcc cgctgcccga
     1201 aaaaccgttt gccattccgc ccgccgcgcc gacgactgcc gtgctctcag ttaaagacgg
     1261 ctggacgaga cggtcttttt gtatcgaaac gattatcggc gaggctttta ttttttccag
     1321 cgccgccgcg tcgacttccg ccgccagaaa gggaataaaa tcgaagcgtt taacgccggc
     1381 gacgttataa ccggaaaggt cggcgagcaa attattctgc gattgcctga ttgcggttcg
     1441 ctgccggttt tgggctgtta aagaaagctt gccctcaatt tgaaaatcgg cgcgcagtcc
     1501 gataataacg cgtaccgaac cgctgcgtcc ggcattttcg attagctcgt caaacttcgt
     1561 cgtcgaatct tgaacggcag ttctctcgtt cgttttttga gaatcgattg gattatttgt
     1621 ccggctcgac tgcatttgcg cgggcatctg ccaaaacatc aaaaaggaaa caatcatcga
     1681 gccaaagatt gcgaggtaaa aaaattttgc tttcatctgc tcctccgaat taggttgaaa
     1741 aacgcgtcgc attaaatcgg ttcgggcgat tttgacagac agtttttgta aggttacata
     1801 atactacacg cgcaatcgaa taccaataaa aacaattgaa aacgaaattc tgccgaggct
     1861 ttgtaaaagc gcccgctaaa gctttaagac gctttgtatt accgggaaat cgactaaatc
     1921 tgcggagaaa tcaacacggt tgccggagaa attttgtccg gctccggaga aattagatcg
     1981 gttttcggag aaagctgctc gctgtcgtga gcggcgctcc gataccgccc tcgtcgctgc
     2041 cgtttattaa agtcgattat ttcgcgcaag ctgccacaaa cttgccaaat gacagaaaag
     2101 cacaatcggg acaatcgtcg aaggcagcca gatgaaaggg aaatacagca cgccgcgatt
     2161 cggctggtcg aaggcgaatt gttgaagcgg gaagggaaac gacaaaacgg cgttgacgac
     2221 gatgtttaaa agaagcccga gcgccagcgt gttccagacg ataagcagcg ggcgatttat
     2281 tttcccgccg cgaaaggcga gccacgccat aacgggcgcg gtgattccgg cgagaatgtc
     2341 gaaatttctg ccttcaaagg tcatcaattg cggaacctgc ccgccctgat aaagccacag
     2401 taaaaccagc tcgaccggaa ttcgaaccac gtgcagataa gtcaaagttt gcagcggaag
     2461 ctgctcgatc aggctttttc gcgcgagaat aaaaagcaga attatcgcgg cgagagccgg
     2521 aaaaagcgca aacagcatca agcgcggcgg cgctatcccg gtatttaaat aaaatccgcc
     2581 gatgccgagc gtggcttgaa aaataagcca gaagggaatc aggaagctta aaaatttggt
     2641 cgcggtcgag gtaaatgcgc cgcgcttgat ggcgtagaga aaaattccga ccgtcaggaa
     2701 tgtcgtcagg ataaacgtaa cggaaacata accgggcaga ttttcgagca taggattcag
     2761 gattcaggat tcaggattca ggatttaaga ttcaagattc aggatttaag attcaaggtt
     2821 cgggattcgg gattcaggat ttgccgttat ccagagacca atcctgaatc ctgaatctta
     2881 aatcctgaat cctgaattac ttaattcttt cgtcgctctc gatttgcccg tcgcggatgc
     2941 tgagaacgcg atgcgcgcgc tcggcgactt cgtgctcgtg cgtgacgacg ataatggtat
     3001 tgccgtcggc gtgcaatttt tcgaacagag ccataatctc gacgctggtt ttcgaatcga
     3061 gcgcgccggt cggctcgtcc gccagcagaa tcgaaggatg attgaccaga gcgcgggcga
     3121 tggcgacgcg ctggcgctga ccgccggaaa gctcgttcgg gcgatgcatg acgcggtcgc
     3181 cgagctcgac cgaagccaga gccgattgcg ctcgctcgtg ccgctggctg gctccgattc
     3241 cggcgtaaat cagcggaagc tcgacattgt gcagcgccgt cgcgcgcggc aaaaggttaa
     3301 acgtctggaa aacaaagccg atttccttgt tgcgaatgcg ggcgagctcg tcgtcggtca
     3361 ttgccgagac cagatgaccg tttatccaat aactgccctg agagggcgaa tcgagacagc
     3421 cgatgagatt catcaaagtc gattttccgg aaccggaagg tccgataatc gccagatatt
     3481 cgccctttgc gacgtcgaac gaaacgtcgc gcagagcgtg cagctcttcc gagcccatct
     3541 ggtatgtgcg ccagatgttc cgcattgaaa tcagcgcgcc cggagttgtt atctcgccgc
     3601 cgccgttagt ttgaatttct gtagccatat tctatggcgg ttttcggttt tcggtcttcg
     3661 gtcttcgcgg gttcaatatt taagcgtaga aattgaaatt acgaagaccg gagaccgaag
     3721 accaaagacc gcttttttac gatttattcg cgttgtcgcc ttcttttttg gtttgtcgct
     3781 tgaccaggtc gttttctttc agcgttttca gaacgcggct cggacccgtt atcacttcct
     3841 gaccttcgga aagaccgctg gtgatttgaa tatcggattc gcctgtaatt ccggtcgtca
     3901 cctcgacaaa tttcacttta ttgccgtcca tcacgtaaac gcctttgatg ggtttcggtt
     3961 tttcgttcga ggcgggcgcg tcgccctgaa cggtcggcga cggcgcggct tcgggctgtt
     4021 tttcgataat cgcctgaagc ggcacggtga cgacgttttc gaccgtcctg gtcgtaatcg
     4081 tcgccgtcgc gctcattccc gggcgcaagc cttcgcgcac cgcgtccgcc acgtctttca
     4141 attgaataac gacgcggaat tcttttgctt cctgcacgtt gatgttggtc gaaagcccgc
     4201 cgctggtttg cgatttgccg agcgccagag gcgtcttctg cgtgacttcg ccgagaattt
     4261 ctttctcgcc gaaagcatcg accttgattt tcgcggtttg cccgacttcg actttgtcga
     4321 tttcggtttc gtcgactttg acctcgacgt taatcgtcga catatcggcg atggtcaaaa
     4381 gcggcgtcga agataaattg gcgacggcga aagtgccgac tttcgacgga atatcggcga
     4441 tcacgccgtt aatcggcgcg gtctgcatac ttttgtcgcg ctggctgcgc gttccgcgaa
     4501 gcagcgcggc ttgctgctcg gcgcgtttcg aggtggcgtc ggcgctgtaa ctcgccgtat
     4561 cgacgccgcg ccgcgcgtct ttgacggcga ccgattgctg attggcgcgg gcgcgggctt
     4621 cgtttacgcg ggcttgcgcc tcgttaactt gtttttcggc gatagtaacg gcatttcttt
     4681 gactctgcag tcgagccaga gcattgcgca aagatatttg agcctggtcg actttatctt
     4741 tgtccgcgtc atattgaagc cgcgaagcga cctgcgcttc gagcagctgc gtcgtccgtt
     4801 tcaactcgcg attagctgtt tccaaatctt ttctggcgcg gtcgacatcg gtctgtgcgg
     4861 tatcgacctg ctgagtggca ttaacaactt gctggcgcgc cgtcgcgacc tgctgcaaag
     4921 ccgtatctat ctgtgcctgc gaggcattta agccctgctg cgcctgtgaa agctgattct
     4981 gagcggcgac gacctgcgag cgcgaaacct gcgtctcggt ctgcgccgcc tgaacggagg
     5041 cgagttgcgc gtcggtgctg gattgcagct ggttagggtc gagacggacg agcggctgtc
     5101 ctttcgtcac ctggtcgcct tccttaacgt aaatttcctc gatgcgtccc tgcacttcgc
     5161 tggtcaggtt gataaactga atcgggcgca cttcgccgga ggctgtgacg gtcgagcgca
     5221 gctcgcggcg ggtttcgatt ttgacgaccg tcacttccgg agtgtcctta cgggtagcaa
     5281 aaatactgcc gataatgata attccgagca aaactacggc aacgctgccg ataatgattt
     5341 tcttctttcg gctcaaagcc atctttttat tcctctaact attgaagggt tttgattctt
     5401 aaagcagttt aagttacgca aataaatttt tacagatgcg tctgcctttc tgatacgact
     5461 tttccggcaa aagagtttca taaaaatgcg ataataaaac ccttgttcga cctttgattt
     5521 ttggcatttg aaaagtcgta tccaatcaaa gcgcgaaaat caaaggtcaa aggtcaaatt
     5581 ggcgatttac gatttacaat ttccgagtta aaatacattc gccatattaa gttcatttct
     5641 acaggcaagc aaggtagagt aaaaatgata aaatgcccaa cttgcgggac ggaaaatctc
     5701 gaaggttcac aattttgtga tgaatgcggt gccgcgctct ccggcgcgag gcaaagaaac
     5761 gactccgaca gtgtcttgac ggcttaccct tcgacgccca attttcaatc ggcaaacgtc
     5821 acgtcggtcg gcgtgccgac ggtcgtcgag cagttgaagg tcgaagaaga cacggaagta
     5881 aaggcagtat cgcaaacgga cgacggcttg tcgaaaggcg ttcacgcggt tttgattatc
     5941 gagcgcggcg cgtcggtcgg aaccgagttc ggcttgacca gcgaagaatc gaacatcggg
     6001 cgctgggacg cggacaacgg aatttttccg gacgtcgatt tggatacgca cgacccggaa
     6061 gccaaagttt cgcgccgcca cgcgcgcatc attcaggaaa acggtcgcta tatgctggag
     6121 gatttaggct cgacgaacgg gacttttatc aatcgcgggc ggcggctgat acccggaaat
     6181 cgacagcctt tgaacgacgg cgacgaagtt atcgtcggaa aa
//
LOCUS       DASWNE010000408         2718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_365849, whole genome shotgun sequence.
ACCESSION   DASWNE010000408 DASWNE010000000
VERSION     DASWNE010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365849"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VGB68_08205"
     CDS             complement(<1..431)
                     /locus_tag="VGB68_08205"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0049250.1"
                     /translation="MTIKSNNNLRRLLEERARERGAKVFLFSEADHRRWTYAEFDKAV
                     NRAANMLLENGIAKGDVVSLLMPNSAEYIIAYFACFKIGALAGPVNSLLKAEEIAFAL
                     KNSEAKMLLVHSQFQKTIDEIREDLPRLQSVIQFDDEAQAT"
     gene            589..2067
                     /locus_tag="VGB68_08210"
     CDS             589..2067
                     /locus_tag="VGB68_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0049251.1"
                     /translation="MTVYRNFINGEWLESVSGEQAPNINPADTDDIIGIIKLSTRDEA
                     RNAVEAAYNAFKGWRNTPAPTRGKTVAKFARLLEDYKEEMAQILTREEGKTLSEARGE
                     LQRSINVAEFCAGESRRLNGETIQSELPANFAYTIKEPHGVVALITPWNFPVAIPVWK
                     IAPALVAGNTVVFKPAEATPATAVRICEIFEEAGLPKGVLNLILGKGSEIGDEIANHP
                     AVKAISFTGSTEIGIKLYEQAARRGAKVQCEMGGKNPVVVMEDADMDLAVESTAQGAF
                     GSTGQRCTATSRAVVVDKIADEFVRKIVERAKRMRLGDGSDSNTEMGPSVDEKQFRTV
                     LKYIDIGREDGAEMLCGGRRAEGDGLENGYFIEPTVFDKVTPDMRIAQEEIFGPVLSV
                     LRVRDFEEAMSVANDSQYGLTSSIFTNDPNLIFRFVDEIETGMTHINSPTTGGEAHIP
                     FGGVKMTGIGAREQGSTALDFYTELKVVYVDYTGRKREGNLY"
     gene            2091..>2718
                     /locus_tag="VGB68_08215"
     CDS             2091..>2718
                     /locus_tag="VGB68_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049252.1"
                     /translation="MNHDQKAGLNKFAALLLFFSFCSIIAAAQARAQTTSLRFETTIA
                     RGLISAPQKGRLFVFLNRRAEPEPRLDDPDVSPDAPPMLAKDVETFAPASTAVVIDND
                     SISYPIKNLAELPAGDYYVQALFDANNDLRSLNAPGNLYSAVQKVSLDARRGGAIKLE
                     LTKIVPPEQLPADTESVKFLKIQSPVLSKFHGRPIFLRVGIILPRDYQS"
BASE COUNT          664 a          716 c          717 g          621 t
ORIGIN      
        1 ttcgtcgcct gcgcttcatc gtcaaattga attacgcttt gcaggcgcgg caaatcttcg
       61 cggatttcat caatcgtctt ttgaaactgc gaatgaacga gcagcatttt cgcttccgaa
      121 ttcttcagcg cgaaagcgat ttcttcggct ttcaaaagcg aatttacggg tcccgccagc
      181 gcgcctattt tgaaacaggc gaaataggcg ataatatatt cggcgctgtt cggcattaat
      241 aaactgacca cgtcgccttt cgcgatgccg ttttccagaa gcatattggc ggcgcgattg
      301 accgctttat caaattccgc gtacgtccag cgccgatggt cggcttcgga aaataaaaaa
      361 actttagcgc ctcgctcccg cgcgcgttct tccagcagcc ggcgcaggtt attgttgctt
      421 tttattgtca tatttcgtat attttagcgc acttcgattt taatttaacg cacaagctgt
      481 ttgaaggaaa aaggaaaaag gtaaaaggct aaagataata aacgaatttt tccttttttc
      541 tttttctttt cgccttgctt tgttttgcgc gtctcaatct taaattttat gaccgtctat
      601 cgaaatttta tcaacggcga atggctcgaa tcagtctcgg gcgaacaagc gccgaatatc
      661 aatcccgcag ataccgatga cattatcggc attataaagc tctcgacgcg cgacgaagcg
      721 cgaaacgcgg tcgaagccgc ctacaatgct tttaaaggct ggagaaatac gccggcgccg
      781 acgcgcggaa aaaccgtcgc caaatttgcg cgcctgctgg aagattataa agaagaaatg
      841 gcgcagattc tgacgcgcga agaaggaaaa acgctttccg aagcgcgcgg cgagcttcag
      901 cgttcaatca acgtcgccga attttgcgcg ggcgaatcgc gccgcttaaa cggcgagacg
      961 attcaatcgg aattgcccgc caattttgct tacacaatca aagagccgca cggcgtcgtc
     1021 gctctgataa cgccctggaa ttttcccgtc gcgattccgg tctggaagat tgcgcccgct
     1081 ttggttgccg gaaataccgt agttttcaag cccgccgaag cgacgcccgc gaccgccgtg
     1141 cgaatttgcg aaatttttga agaagccgga ctgccgaaag gcgttctgaa tttaattttg
     1201 gggaaaggct cggaaatcgg cgacgaaatc gccaatcatc cggcggtcaa ggcgatttct
     1261 ttcaccggct cgaccgaaat cggcattaaa ttatatgagc aggcggcgcg gcgcggcgcg
     1321 aaagtgcaat gcgagatggg cggcaaaaat ccggtcgtcg tgatggaaga cgcggatatg
     1381 gatttggcgg tcgaatcgac ggcgcagggc gctttcggct cgaccggaca acgctgcacg
     1441 gcgacctcgc gcgccgtcgt cgtcgataaa atcgccgacg agtttgtccg taaaatcgtc
     1501 gagcgagcga aacgaatgcg cctcggcgac ggcagcgatt caaacacgga aatgggacct
     1561 tcggttgacg aaaaacaatt caggacggtt ttgaaatata tcgacatcgg gcgcgaagac
     1621 ggcgcggaaa tgctctgcgg cggcaggcgc gccgaaggcg acgggctcga aaacggttat
     1681 ttcatcgagc cgacggtttt tgataaagtc acgccggata tgcgcattgc gcaggaggaa
     1741 attttcggtc cggttctctc ggtcctgcgc gtcagggatt ttgaagaagc gatgagcgtg
     1801 gcaaacgatt cgcagtacgg tctgacttct tcgattttca ccaacgaccc gaatctgatt
     1861 ttccgttttg tcgacgaaat cgaaaccgga atgacgcaca tcaactcgcc gacgaccggc
     1921 ggcgaagcgc acattccctt cggcggcgta aaaatgaccg gcatcggcgc gcgcgaacaa
     1981 ggctcgaccg cgctcgattt ttacaccgaa ctgaaagtcg tctacgtcga ttacacggga
     2041 cgcaagcgcg aaggcaattt gtattagttg gaaattcgag gagtaaagaa atgaatcacg
     2101 accaaaaagc cggacttaat aaatttgccg cccttctgtt atttttttcc ttctgctcga
     2161 ttattgccgc cgcacaagcc cgggcgcaga cgacctcgct gcgatttgag acgaccattg
     2221 cgcgcgggct tatctccgct ccgcaaaaag ggcgtttgtt tgtttttctc aatcgccgag
     2281 ccgagcccga gccgcgattg gacgacccgg acgtcagccc ggacgcgccg ccgatgctgg
     2341 cgaaagacgt ggaaactttc gcgcccgctt cgaccgcggt cgtgattgac aacgattcaa
     2401 tatcgtatcc gattaaaaat ctcgccgaac tgcccgccgg agattattat gtacaggctt
     2461 tgtttgacgc caacaatgat ttgcgctcgc tcaacgcgcc cggaaattta tacagcgcgg
     2521 ttcagaaagt cagcctcgat gcgcgccgcg gcggagcaat caaactcgag ctaacgaaaa
     2581 tcgtgccgcc cgaacaattg cccgccgaca ccgaatcggt caaattcctg aaaattcaat
     2641 cgccggtgct gagtaaattt cacggcagac cgatttttct gcgcgtcgga atcattctgc
     2701 cgcgcgatta ccaaagcg
//
LOCUS       DASWNE010000409         1946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_365874, whole genome shotgun sequence.
ACCESSION   DASWNE010000409 DASWNE010000000
VERSION     DASWNE010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365874"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            269..1150
                     /locus_tag="VGB68_08220"
     CDS             269..1150
                     /locus_tag="VGB68_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEY0049253.1"
                     /translation="MSIKQVDVYRLAEPLDAPASLTEEDFASRSTLIGSVSITEEDLV
                     RKQIIYTDSLEFAGQAVKLRYAIRFVNSAGQKAGFSNFLLIEPTAKVAGSPSLSAPAV
                     SQEAVLLSWQPPASNVDGSKPVNILGYNVYRSEKETPQSRMLNSAPVTGNEFSDKLFE
                     FERNYRYFIRAVSLGTNGQPIESANSNAVEIVPRDTFVPNAPTALTIAATPDTISIFF
                     ATNLEKDIAGYRIYRSTNPDLPKSEWQNLTPNLLTANTFQDTKIESGKTYFYYLTAVD
                     KFGNASEPSEVVSETVP"
     gene            1157..1933
                     /locus_tag="VGB68_08225"
     CDS             1157..1933
                     /locus_tag="VGB68_08225"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY0049254.1"
                     /translation="MKICRFINKNQEQYGALHNNRVFPFPDTNEFGDLSETLKLIESF
                     ELSEIKLLPPATPTKIVCVGRNYAEHAAELGNEVPKEPLLFLKAPSALITEGEAIVIP
                     EQSKQVEHEGELAVVIGRECGNLSENDNPFEYVFGYTCLNDVTARDLQRRDVQFTRAK
                     SFDTFCPIGAFIETELDVSDLRVVTKVNGLVKQDGRTSQMVFPVDFLIRYISNMMTLK
                     AGDIIATGTPSGVSKLNPGDLCEVEIEGVGSLRNPVEEGI"
BASE COUNT          535 a          466 c          444 g          501 t
ORIGIN      
        1 gaacctgagc cagtatatcc tgtagcttaa aagaatgctt aaccaattat taaaccttac
       61 atatttaagc agatgctttc cgcctaaaac agcattagcg tttataatat ttgtttcgct
      121 tatttttatc ggctgcggca aaagaaaaat tccactgccg ccggtcgaaa aagttcagca
      181 gcgagtcgag atatcaggtt ttcaacaagg caacaaagta aatctgtcct gggcgcttcc
      241 ctctcagaac gtttccgatt ccagcgtttt gagtattaaa caggttgacg tttatcgcct
      301 ggcggaaccg ctcgacgcgc ccgcttcatt gacggaagaa gattttgctt cgcgcagcac
      361 gctgatcggg tcagtgtcta ttaccgaaga agatttggtt agaaaacaaa ttatttatac
      421 cgactcgctc gaatttgccg gacaagccgt caagcttcgt tatgccattc gttttgttaa
      481 ttcagccgga caaaaagccg gtttttccaa ttttttatta atcgagccaa ccgcaaaagt
      541 tgccggttcg ccatcgctct ccgcgcctgc ggtatcgcag gaagcggttt tgcttagctg
      601 gcagccgccg gcatcgaacg tagacggatc aaagccggta aatattttgg gctataacgt
      661 gtatcgctct gaaaaagaaa cgccgcagag cagaatgctg aattccgctc cggttaccgg
      721 aaacgaattc agcgataagc ttttcgagtt tgaacgaaat taccggtatt ttatacgcgc
      781 ggtttcgctc ggaacgaatg gtcagccgat tgagagcgcc aattcgaatg ctgttgaaat
      841 cgttcccaga gacacatttg tgccgaacgc gccgaccgct ttaactattg ccgcaacgcc
      901 cgatacaatc tcgatattct ttgccacgaa tcttgaaaaa gatattgccg gttatcgcat
      961 ttatcgctcg acaaatcccg atttgccgaa atccgaatgg caaaacctga cgccgaatct
     1021 tttgaccgcc aatacttttc aggacactaa aatcgaatcc gggaagacat atttttatta
     1081 tctgacggcg gtcgataaat tcggtaatgc gagcgagccg tcggaggtgg tcagcgaaac
     1141 cgtcccataa aattttatga aaatctgtcg ttttataaat aaaaatcaag agcaatacgg
     1201 cgcgctgcat aacaatcgcg ttttcccttt tcccgataca aatgagtttg gcgacctgag
     1261 cgaaaccctg aagctgattg aaagcttcga attatccgag ataaaacttc ttcctccggc
     1321 gacgcctaca aaaatcgtct gcgtcggcag aaattacgcc gaacacgccg ccgagctcgg
     1381 caacgaagtg ccgaaagaac cgcttttgtt tttgaaagcc ccgtcagctt taattactga
     1441 aggcgaagcg attgtaattc ccgaacaatc taaacaggtt gagcacgagg gcgagctggc
     1501 tgtcgttatc ggacgcgaat gcggcaattt atccgaaaat gataacccgt ttgaatacgt
     1561 tttcggctac acctgtctga acgacgtaac cgccagagat ttacagcgca gagacgttca
     1621 gtttacgcgc gctaaatcat tcgacacatt ttgtccaatc ggcgcattta tcgaaaccga
     1681 gcttgacgtt tccgaccttc gcgtcgtgac gaaagtcaac gggcttgtaa aacaggacgg
     1741 gcggacttcg cagatggttt ttccggttga ttttttgata cgatatatct caaatatgat
     1801 gactttaaaa gcgggcgaca tcatcgcaac cggaacgcct tccggcgttt ctaaattaaa
     1861 ccccggcgac ctttgcgaag tggaaattga aggcgtcggc agcctgcgaa atcccgtcga
     1921 agaagggatt taacaaagta atattt
//
LOCUS       DASWNE010000410         5220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_326270, whole genome shotgun sequence.
ACCESSION   DASWNE010000410 DASWNE010000000
VERSION     DASWNE010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326270"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..1556
                     /gene="murC"
                     /locus_tag="VGB68_08230"
     CDS             177..1556
                     /gene="murC"
                     /locus_tag="VGB68_08230"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEY0049255.1"
                     /translation="MFRHVKHVHFIGIGGIGMSGIAEVLCNLGFKISGSDLKKSGNTD
                     RLELSGATVFEGHAAENVGDAQVVVYSSAVRADNPEVVEAKSRSIPVIPRAEMLAELM
                     TLKPYSVAVAGSHGKTTTTSMVATILGHAGVDPTTIVGGVVDTLGSNARLGQSDWFVT
                     EADESDRSFLMLSPTVALVTNVDREHMDSYKGMEDVMQCFMDFVNKVPFYGAAVLCLD
                     DPNVQAIIPHIKRRRVTYGFSAQADVSAHNIRYNDSFGSTFTVWKGAEVLGEISLPVP
                     GKHNVYNALGATAIALELDVEFEMIAGAFKTFKNANRRFQFKGEAKGVTVVDDYGHHP
                     TEILATLAAARNGSGGRRTVVIFQPHRYTRTQDLMDDFARSFNNADVVFLTDIYAASE
                     MPIEGITSEVLTAKIKQYGHKNVQYIGDIKGAAEKVNEQLREGDLVITLGAGSVTKLS
                     EQILEILKR"
     gene            1637..2497
                     /locus_tag="VGB68_08235"
     CDS             1637..2497
                     /locus_tag="VGB68_08235"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEY0049256.1"
                     /translation="MTKQTRKQRTTTTTNAKRRTAAAAPAKRRGAAARRGKSPSRLTM
                     TVVPLFLILCILTCLGFLTFMGYRTVTASAFFDMKKVEVRGAHRVSPEEIEKIVRAGM
                     AKSSVWNANVDEIRTEIEKLPFVKSVAVSRVLPDGVRVNVTERTPRAVVRLNSGDFWV
                     DDDALVLGKTEKSDLQNAFILRGWDEAKTDKALKDNQERVRLFQKIKEDLQKAGIAER
                     VTAINLTYLQDAQVTVEDSGETVSVFLGREDFGKRLRTALENLAGRGKEIGILISHGS
                     NPIARFRANS"
     gene            2621..3865
                     /gene="ftsA"
                     /locus_tag="VGB68_08240"
     CDS             2621..3865
                     /gene="ftsA"
                     /locus_tag="VGB68_08240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEY0049257.1"
                     /translation="MNRSIQAVGLDIGTSKVRCVIGEPSEKGSMNIVGIGQVDSRGLR
                     RGIVTTTDAVAEAIKRAVEEAERVSGLEVQMATINLSGEHFRGENKHGVVAVAGAGRE
                     ISVEDVERAVESASAMQLPAGWEIIDRVPQEFIIDGQDGITEPIGMIGARLESRVHIV
                     SSPSAGRQNVVKAIHNSGLDIEKMMLEPLAAAEAVLTDDDREYGCALVNMGAEVTSLM
                     TFGRGAVQHTAVFPFGGMHFTKDIAHGLRVSIPEAEKIKKRFGCIASFLMDENERQEI
                     IEITPVGRNETRGLSKEILCDIMQPRAIELLQHIAHEIMQAGGNGQISSGVVLTGGSA
                     MIRGTVEIAEQVFDAPTRVGFPDRRRFGGLVDEVQTPAWSVACGLAWSSMRSQMRGQS
                     PGGRSTTKKVAEWFENFREKFR"
     gene            4047..5165
                     /gene="ftsZ"
                     /locus_tag="VGB68_08245"
     CDS             4047..5165
                     /gene="ftsZ"
                     /locus_tag="VGB68_08245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEY0049258.1"
                     /translation="MNNNGIKIFIDEPPITGARIKVIGVGGGGSNAVNRMIEAGIKGV
                     EFIVANTDVQALNNSNAPIKIQLGSKLTRGLGAGSDPLVGRDAALEDHEKIMDVLEGS
                     DMVFVTTGLGGGTGTGAAPVIASLAIELGALTVAVVTKPFTVEGKKRMLQAERGLAEL
                     RGCVDTIITIPNSRLKEVEEKISIMEAFRRADDVLLQAVQSISELITESGMINLDFAD
                     VKTVMKGMGVALMGIGHAKGDNAAVKAMKAAIDSKLLEENSIKGAKGALVNITGSMDM
                     PLNEVEDALALIHDEAHEDASIIFGTVFDENMEDEVKITVIATGFDNQSQVALPTSAL
                     TDTQAVSSQYGVRPALKPIEKPREEDINVPTFIRRQAD"
BASE COUNT         1283 a         1296 c         1543 g         1098 t
ORIGIN      
        1 gcgcccgcgc ggattacgga aatggaagct tccgcccgaa aattggcgaa agccgatgcg
       61 gcggaggtca cagtggattt aatagaggag ttagcaagga gaaattgatt gcagattaca
      121 gattacagat ttttcgaaaa atctaaaatc tgaaatctaa aatccaaaat caaagaatgt
      181 ttcgtcacgt aaagcatgtt cattttatcg ggattggcgg catcggaatg tccggcatcg
      241 cagaggtttt gtgcaatctc ggttttaaaa tttccggctc ggatttgaaa aaatccggca
      301 ataccgaccg tctggagctt tcgggcgcga ccgttttcga agggcacgcg gcggaaaatg
      361 tcggcgacgc gcaagtcgtc gtttattcgt cggcggtcag agcggacaac ccggaagtcg
      421 tcgaagcgaa atcgcgctcg attccggtta ttccgcgcgc ggagatgctg gcggaactga
      481 tgactctgaa gccttacagc gtcgccgtcg ccggctcgca cggcaagacg acgacgactt
      541 cgatggtggc gacgattctc ggacacgccg gagtcgaccc gacgacgatt gtcggcggcg
      601 tggtcgacac gctcggctcg aacgcccgct taggacaaag cgattggttc gtcaccgaag
      661 cggacgagag cgaccgctcg ttcctgatgc tctcgccgac cgtggcgctg gtgacgaacg
      721 tcgaccgcga gcatatggac tcctacaagg gaatggaaga cgtgatgcag tgttttatgg
      781 atttcgtcaa caaagttcct ttttacggcg cggcggtttt atgtctggac gacccgaatg
      841 tgcaggcgat tattccgcac atcaagcgcc gccgcgtgac ttacggattt tccgcgcagg
      901 cggacgtttc ggcgcacaac attcgctata acgattcgtt cggctcgact tttacggtct
      961 ggaaaggcgc cgaagtgctc ggcgagataa gcctgcccgt gcccggtaag cacaatgttt
     1021 acaacgctct cggagcaact gccatcgcgc tggaactgga cgtcgaattt gaaatgattg
     1081 ccggcgcgtt taagactttc aaaaatgcca atcgccgttt tcagtttaaa ggcgaggcga
     1141 aaggcgtgac ggtcgtcgat gattacgggc atcacccgac ggagattctg gcgacgctgg
     1201 cggcggcgcg aaacggttcg ggcgggcggc gcacggtcgt gattttccag ccgcatcgct
     1261 acacgcgcac gcaggatttg atggacgatt ttgcgcgctc gttcaataac gccgacgttg
     1321 tgtttctgac ggatatttat gcggcgagcg agatgccgat cgaaggaatc acttctgaag
     1381 ttttgaccgc gaaaatcaag caatacggtc acaaaaacgt gcagtatatc ggcgacatca
     1441 aaggcgccgc cgaaaaagtc aacgaacaat taagggaagg agatttggtg attactctcg
     1501 gcgcgggaag cgtgacaaag ctttccgagc agattctgga gattttgaaa aggtagttaa
     1561 ccgaagttga ctgagttgac agagtttttg atttgaactc agtcaacttc cagtcaacct
     1621 tccgaactta agaattatga caaaacaaac gcgaaaacaa cgaacgacca cgacgaccaa
     1681 cgccaaacgc aggacggcgg cggcagcgcc cgccaaacgt cgcggcgcgg cagcacgccg
     1741 cggcaaatct ccgagccgtc tgacgatgac ggtcgtgccc ttatttttga ttttgtgcat
     1801 tctgacctgt ctcggttttc tgacgtttat gggctaccgg acggtgacgg cttcggcgtt
     1861 tttcgatatg aaaaaagtcg aagtgcgcgg cgcgcatcgc gtctcgcccg aggaaatcga
     1921 gaaaatcgtc cgcgccggaa tggcgaaatc gagcgtctgg aacgcgaatg tcgatgaaat
     1981 caggacggag attgaaaagc ttccgtttgt gaaatcggtc gccgtctcgc gcgttttgcc
     2041 cgacggcgtt cgcgtcaatg tgacggaaag gacgccgcgg gcggtcgttc gccttaattc
     2101 cggcgatttc tgggtcgacg acgacgcgct ggttttaggc aaaaccgaaa aaagcgattt
     2161 gcagaatgct tttatcctgc gcggctggga tgaagcgaaa accgacaaag cattaaagga
     2221 taatcaggaa cgcgtcaggc tttttcagaa aataaaagag gatttacaaa aggcgggcat
     2281 tgccgagcgc gtgacggcga taaatctgac ttacttgcag gacgcgcagg tgacggtcga
     2341 agattcgggc gagacggttt ccgtctttct gggcagggaa gatttcggca agcgcctgcg
     2401 caccgcgctg gaaaatcttg ccggcagagg caaggagatt ggaattttga tttcgcacgg
     2461 cagcaatccg attgcccgtt ttcgcgcaaa ctcctgagat tttagatttt agatttttga
     2521 tttcagattg gataaggaat ttatcttttt ttgatttatt cggctcgcgc cggatgccgc
     2581 ttccaatcgc aaatctaaaa tcgaaaatcg aaaataattt atgaatcgtt ctatacaagc
     2641 agttggatta gacatcggca ccagcaaggt tcgctgcgtt atcggcgagc cgtcggaaaa
     2701 aggctcgatg aatattgtcg gcatcgggca ggtcgattca aggggcttgc gacgcggaat
     2761 cgtgacgacg accgacgcgg ttgccgaagc aatcaaacgc gccgtcgaag aagccgagcg
     2821 cgtgagcggc ttggaagtgc agatggcgac catcaatctt tcgggcgagc attttcgcgg
     2881 cgagaataaa cacggcgtcg tcgccgtcgc cggcgcggga cgcgaaattt cggtcgaaga
     2941 tgtcgagcgc gccgtcgaat cagccagcgc catgcagctt ccggcaggct gggaaattat
     3001 cgaccgcgtg ccgcaggaat ttattatcga cgggcaggac ggaatcaccg agcctatcgg
     3061 aatgatcggc gcgcggctcg aaagccgggt tcacatcgtc agcagtccga gcgccggacg
     3121 acagaacgtc gtcaaagcga ttcataattc cgggctcgat attgaaaaaa tgatgctcga
     3181 gccgctcgcc gcggcggaag cggttttgac cgacgacgat agggaatacg gctgcgcgct
     3241 cgtcaatatg ggagcggagg tgacgagcct gatgactttc gggcgcgggg cggtacagca
     3301 caccgccgtt tttccgttcg gcgggatgca ttttaccaaa gacatcgcgc acgggcttcg
     3361 ggtttcgatt ccggaagccg aaaaaatcaa gaagcgtttc ggctgtatcg cttcgtttct
     3421 gatggacgaa aacgagcggc aggaaattat cgaaatcacg cccgtcggca gaaacgagac
     3481 gcgcgggctg tcgaaagaaa ttttgtgcga tattatgcag ccgcgcgcta tcgaattatt
     3541 gcagcacatc gcgcacgaaa ttatgcaggc gggcggaaac gggcagattt cgagcggcgt
     3601 cgtgctgacc ggcggcagcg cgatgattcg cggcacggtc gagattgccg agcaggtttt
     3661 tgacgccccg acgcgcgtcg gttttcctga ccgccgacgt ttcggcggct tggtcgatga
     3721 ggtgcagacg cctgcctggt cggtcgcctg cgggctcgcc tggtcttcga tgcgctcgca
     3781 aatgcgcggg cagtcgccgg gcggaaggtc aacaaccaaa aaggttgcgg agtggtttga
     3841 aaattttcga gaaaaatttc gttaatttag acgaaattaa catttctggc tggatttttt
     3901 gtcacaaatc gttgtatgct ggttcgccgt agacacaata tcttgttaga atgcactaaa
     3961 gcggtcttct cgaacatcac cacgggtagt ttcagtaaaa tactatttaa agggaagcat
     4021 ttatcatgtc aacggacggt cataaaatga ataacaacgg aatcaaaatc tttattgacg
     4081 agcctccgat tacgggcgcg agaatcaagg ttatcggtgt cggcggcggc gggagtaacg
     4141 ccgtcaatcg aatgattgaa gccggcatca agggggtcga attcatcgtc gccaacacgg
     4201 acgtgcaggc gctcaataac tcaaacgcgc cgataaaaat ccagctcggc agcaaactca
     4261 cgcgcgggct tggcgccggc tccgacccgc tcgtcgggcg cgacgccgcg ctggaagacc
     4321 acgaaaaaat tatggacgtg ctggaaggct cggatatggt tttcgtgacg accggattag
     4381 gcggcggaac gggaacggga gccgcgcccg taatcgcctc gctcgccatc gaactcggcg
     4441 ctttgaccgt cgccgtcgtg accaagcctt ttaccgtcga aggcaaaaaa cgaatgctgc
     4501 aagccgagcg aggacttgcc gagctgcgcg gctgcgtcga tacgattatc acgattccga
     4561 attcgcgcct gaaagaagtc gaagaaaaaa tctcgattat ggaagcgttt cggcgcgccg
     4621 acgacgtcct tctgcaagcc gtccagagca tcagcgaatt gattaccgaa tccggtatga
     4681 ttaatctcga ttttgccgac gtgaaaaccg tgatgaaggg aatgggcgtc gcgctgatgg
     4741 gaatcggaca cgccaaaggc gacaacgccg ccgtcaaagc gatgaaagcg gcgattgatt
     4801 ccaagctttt ggaagaaaat tcaatcaaag gcgcgaaggg agcgctggtc aatattaccg
     4861 gctcgatgga tatgccgctc aacgaagtcg aagatgcgct cgcgctgatt cacgacgaag
     4921 cgcacgaaga cgccagcatt attttcggca cggtcttcga cgaaaatatg gaagacgaag
     4981 tgaaaatcac agtcatcgcc accggtttcg acaaccaatc gcaggtcgct ctgccgactt
     5041 cggcgctgac cgatacgcag gcggtcagca gtcaatacgg cgtgcgtccg gcgctcaagc
     5101 cgattgaaaa gccgcgtgaa gaagacatca acgtgccgac ttttatcagg cgtcaggcgg
     5161 attaaaaaga gtcgcgggca gggagtcagg tgtttggagc aaagaaagct cgtcccgact
//
LOCUS       DASWNE010000411         7543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_366026, whole genome shotgun sequence.
ACCESSION   DASWNE010000411 DASWNE010000000
VERSION     DASWNE010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7543
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366026"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1244
                     /gene="asnB"
                     /locus_tag="VGB68_08250"
     CDS             <1..1244
                     /gene="asnB"
                     /locus_tag="VGB68_08250"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEY0049259.1"
                     /translation="TFKNGEIKTRKYWDFDYRKVSGIKTEAEYIEILREKIKDAVKVR
                     LISEVPLGAFLSGGVDSSSVVAMMSQILDAPVKTFSIGFNEDSFNELKYARVAAEHFQ
                     TEHHEFIVTPDLVETVDELVWHFDEPFADSSALPTFMVSKMARDYVTVVLSGDGGDEL
                     FAGYTRYVVDRKRSGMANLPSFVRRNLRAVSESLPHGAKGRNYLYNVSLDALDRYIDS
                     ISQYNALRKKSLYSKDFQSRLNGNSGASVYRNIAESVSTGNAIDNLLYLDSKTYLPGD
                     ILTKVDRMSMAASLEARVPLLDHELIEFVTQIPPDLKMKGFETKYIFKKAMEGIVPKE
                     ILYREKQGFGVPIEEWINNQLRARIHETLLDARTLQRGYFEKSYIQTLLDEHSKSRRD
                     HSHALWILWMLELWHRRFLDK"
     gene            1304..2503
                     /locus_tag="VGB68_08255"
     CDS             1304..2503
                     /locus_tag="VGB68_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049260.1"
                     /translation="MSKAPNNLLTKLKSNLPWLARYPLVRAKSLLSRNLFEKKHVIFV
                     VANHFEPSWKAKGFHDSDSQLRRLDEYHKLARATGEAVRDADGTKFRHTNFYPAEQYD
                     ARLLEKMAEMQAEGLGEVEIHLHHGIERADTAENLRKSLTEFRDILAERHQCLSRLDG
                     AGSPMYAFVHGNLALANSCGGKFCGVDEEMRILQETGCYVDMTLPSAPDETQVPVINQ
                     IYECGLPLNEKMPHRKAIPARVYGNQPQLPLIFQGPLVFNWTRRIKGLPVPRLDDGAL
                     TYNQPSDLARLNRWLSANVTIEGKSDWVFVKLYCHGFFDYDQSACIGEDAQRFFGEAV
                     ENGEKTGKYKVHFATAREAFNMAAAAIDGKKGTPNDFRNYRLRAIMDEKKIFNAKAHE
                     TVVSAKR"
     gene            2573..5035
                     /locus_tag="VGB68_08260"
     CDS             2573..5035
                     /locus_tag="VGB68_08260"
                     /inference="COORDINATES: protein motif:HMM:NF028199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEY0049261.1"
                     /translation="MANPLDKIRKLKGRSFKEIRSRGGQALSVYTEQIGLSGKLPTDE
                     EFQQLIDQSFFKEKTPTARELFEVFFKHGNQAFFQSFAQKEKTLEAFQSLAAESRFSI
                     IEQAEQMIEGKFDLLGFKNLDFESPVNWHFEPIAGKHSPLKHWKQFDELDAAETGDKK
                     IVWELNRHQHFFTLGAAFWLTGDENYAEAFARHIDGWMEQNPPGLGLNWFSSLEVAFR
                     AISWLWAFHFFKDSRSFSPALFHKALKFLYVHGRHIEKYLSTYYSPNTHLTGEALGLY
                     YLGTQLPFLQAARAWRETGAEILFAELNRQILPDGVYFEQSTWYARYTADFYTHFLIL
                     KALSEEESDRETEEKLAGKLQLLLNFLMQVTRPDGTTPLIGDDDGGRSLPFGYNHSAP
                     NDFRAVLSTGAVIFERGDYKFVAREAAEETLWLLGSEGIDGFEDLGELRPIHNSKSFP
                     AGGYFVMRDGWDTTDNYFLIDCGNVGAMSGGHGHADTLAFDLASGGRTLLIDAGTYTY
                     HESEDLRNHFRSTAAHNALTIDNESSSQTGGKFGWRTMAKPQVNAWIAENRFDFFEGE
                     HDGYRHLPEAPATHARSVLFLKNDYWIMRDFVETKGAHDYQLNFHFNPQTAPKIVGGE
                     NGSFYVDEETDKNSGLRIGIIGDNGGWQMSENWISDCYGRRAGAPFLRFQSKGAAAQE
                     FFTFLFPIDSLSEKPEIFETEIAGGRAFVVKFRGYTDIFVYADGDRIVRTEFFDTDFR
                     FVWARLSEGEDMPEEFVLLDGKNFVVNSREIVNYPQRLKFAAARRLGSKLNVKTSESL
                     FSVSLPARRSHRYTLKNSEQSE"
     gene            5184..6326
                     /locus_tag="VGB68_08265"
     CDS             5184..6326
                     /locus_tag="VGB68_08265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0049262.1"
                     /translation="MKKNILQLVGSFHSGGSERQAAQLIRLLQEDKTHRIFVACLNGA
                     GVLRGSVEKLGFAEIPEFRLTSFYDANMARQLSRCRAFLQENRIDIIQTHDFYTNVFG
                     MTAGRMARVPVRVAAKRETGTKSSAQRFVERRAYAFAHAVVANASAVKNHLIETGVAA
                     KKIEVVYNGLELAKFDSKNEPRAEILKSFGLPPDENLKFVTIVANFRSDVKNHPMFLR
                     AARSVKENFPAARFLLAGEGELLIEMKDLAKDLGLENESFFIDNCGDVPRLLLASDIC
                     VLSSRTEGFSNSILEYMAAGKPVVATDVGGAGEAIDEGENGFLVESNDDASMANRLLE
                     LLRDASKAEIFGARGRAIAEAKFSTAAQLKKTLDLYDRLLQNGERK"
     gene            6418..>7543
                     /locus_tag="VGB68_08270"
     CDS             6418..>7543
                     /locus_tag="VGB68_08270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0049263.1"
                     /translation="MSHLQMNQSSEKTKILIIAPALPLVGGQAVQAQRLLENFRREPH
                     LEVDLQPINPQVAPRLQKIPYVRTAVTSLKYLFDLFGKIPRADVVHIFSAAYYGFVLS
                     VVPAILLAKLFGKKTIINYRSGEAENFFRGWGGYFLWAVRMADAVVSPSGYLVDVFEQ
                     FGVRGRSIFNIIDTDKFTFRARRALRPIFLSNRILESLYNIECIFRAFRTVSEKFPDA
                     RLIVANDGIEREKLERAAREMNLKNVEFIGLVPQDKIAALYDSADVYLNSPNFDCMPG
                     SLIECFASGLPVVSTNAGGIPYIVADGETGLLVETNDDAALAAKAIYLLENPEAAQAL
                     IDNARKECAKYSWAAIRPQWLALYEKLAAELKGNPEPQKLL"
BASE COUNT         1814 a         1902 c         2070 g         1757 t
ORIGIN      
        1 tgactttcaa aaacggcgaa atcaaaacgc ggaaatactg ggatttcgat taccggaaag
       61 tttccggcat caaaaccgaa gccgaataca tcgagattct gcgcgagaaa atcaaagacg
      121 ccgtcaaagt tcgtttaatc tcggaagttc cattgggcgc gtttttgtcg ggcggcgtcg
      181 attcttcgag cgtcgtcgcg atgatgtcgc agattctgga cgcgccggtc aaaacttttt
      241 ccatcggatt taacgaggac agctttaacg agctgaaata cgcgcgcgtc gccgccgaac
      301 atttccagac cgagcatcac gaatttatcg tcacgccgga tttggtcgag acggttgacg
      361 agctcgtctg gcattttgac gagccgtttg ccgattcttc cgcgctgccg acatttatgg
      421 tctcgaaaat ggcgcgcgat tacgtgacgg tcgttttgtc gggcgacggc ggcgacgagc
      481 ttttcgccgg ctacacgcgc tacgtcgtcg accgcaagcg aagcggaatg gcgaatctgc
      541 cgtcgttcgt cagacgaaat ctgcgcgccg tcagcgagag tttgccgcac ggcgcgaaag
      601 gcagaaatta tctttacaac gtctcgctcg acgccctgga ccgctacatc gacagcattt
      661 cgcaatacaa cgcgctgcga aaaaaatcgc tttattcgaa agattttcag tcgagactga
      721 acggaaattc cggcgcgagt gtttatcgaa atatcgccga gagcgtctcg accggaaacg
      781 ccattgataa tctgctttat ctggacagca aaacttatct gcccggcgac attctgacga
      841 aagtcgaccg gatgagtatg gcggcgtcgc tcgaggcgcg cgtgccgctt ctcgaccacg
      901 aattgattga attcgtcacg cagattccgc ccgatttgaa aatgaaaggc ttcgaaacca
      961 aatacatttt caaaaaagcg atggaaggca tcgttccgaa ggaaattctc taccgcgaaa
     1021 aacagggctt cggcgtgccg attgaagaat ggataaacaa tcagctgcgc gcgcggatac
     1081 acgaaaccct gctcgacgcg cgcactttac agcgcggata ttttgaaaaa tcctatattc
     1141 agacgctgct cgacgaacac tcaaaaagcc gccgcgacca ttcgcacgcg ctctggattt
     1201 tgtggatgct cgagctctgg caccggcggt ttctggataa atgattttat tcgcgctttt
     1261 tacgctacag aaagcacgct cgattgtgct ttaatgatga aaaatgagca aagcgccaaa
     1321 caatctcctg accaaattaa aatcaaatct gccgtggctc gcgcgttatc ctctggtgcg
     1381 cgccaaatcg cttttaagcc ggaatttatt cgagaaaaaa cacgttatct tcgtcgtcgc
     1441 caaccatttc gagccgagct ggaaggcgaa aggctttcac gattcggatt ctcagctgcg
     1501 ccgtctcgac gaatatcaca aactggcgcg cgcaaccggc gaagccgtgc gcgacgcgga
     1561 cggcacgaag tttcgtcaca cgaattttta tccggcggag caatacgacg cgcgcctgct
     1621 ggaaaaaatg gcggaaatgc aggcggaagg tttgggcgag gtcgaaatcc atctgcatca
     1681 cggcatcgaa agggcggaca cggcggaaaa tctgcgaaaa tctttaaccg agtttcgaga
     1741 tattttagcc gagcggcacc aatgcctttc gcgtctggac ggcgcgggaa gcccgatgta
     1801 cgcgtttgtt cacggcaacc tggcgctggc aaattcctgc ggcggaaagt tttgcggcgt
     1861 cgacgaggaa atgcggattt tgcaggaaac cggctgttac gtcgatatga cactgccgtc
     1921 cgcgcccgac gagacgcagg ttccggtcat caatcaaatt tacgaatgcg gcttgccttt
     1981 aaacgaaaaa atgccgcatc gaaaagccat tccggcgcgt gtttacggaa atcagcccca
     2041 attgccgctg atatttcagg gaccgctggt ttttaactgg acgcggcgca tcaaaggatt
     2101 gcccgtcccg aggctggacg acggcgcgtt gacttataat cagccgtcgg atttggcaag
     2161 gctgaatcgc tggctttcgg caaacgtgac aatcgaggga aaatcggatt gggttttcgt
     2221 caagctttat tgtcacggtt ttttcgatta cgaccaatcg gcttgcatcg gcgaagacgc
     2281 tcaaagattt tttggcgaag cggtcgaaaa cggcgagaaa accggcaagt acaaagttca
     2341 ttttgcgacg gcgcgcgagg cgttcaatat ggcggcggcg gcgattgacg gcaaaaaggg
     2401 aacgccgaac gattttcgca attatcgtct ccgggcgata atggatgaaa agaagatttt
     2461 taacgcaaag gcgcacgaaa cagtggtcag tgctaagcgg taaacggtaa gttcgatact
     2521 ttttaattgc aactaaccac ttacccttta ccgtttacca ctggaaaaat tgatggcaaa
     2581 tccgctcgat aaaataagaa agctgaaagg gcgcagtttc aaggaaatcc gctcgcgcgg
     2641 cgggcaggct ttatccgtct acaccgagca aatcggtctg agcggaaagc tcccgaccga
     2701 cgaagaattt caacagctca tcgaccaatc ctttttcaag gaaaagacgc cgaccgcgcg
     2761 cgagcttttt gaagtctttt tcaaacacgg caatcaggcg ttttttcaat ccttcgcgca
     2821 aaaagaaaaa actctggaag cgtttcaaag cctggcggcg gaaagccgct tttcgattat
     2881 cgagcaggcg gagcaaatga ttgaaggcaa attcgatttg ctcggcttta aaaatctcga
     2941 tttcgaatcg cccgtcaact ggcatttcga gccgattgcc ggcaaacatt cgccgctcaa
     3001 gcactggaaa caattcgacg agctggacgc tgcggaaacc ggcgacaaaa aaatcgtctg
     3061 ggagctgaac cggcaccagc atttctttac gctcggcgcg gcgttctggc tgaccggcga
     3121 cgagaattac gcggaagctt tcgcgcgtca tatcgacggc tggatggagc agaatccgcc
     3181 gggcttgggg ctcaactggt tcagcagtct ggaagtggcg tttcgcgcga tttcgtggct
     3241 ctgggcgttt cattttttca aggattcgcg aagcttttcg cccgcgcttt tccacaaagc
     3301 cctgaaattt ttgtatgttc acggcaggca catcgaaaaa tatctctcga cttattacag
     3361 cccgaacacg catctgacgg gcgaggcgct cggactttat tatctcggga cgcagctgcc
     3421 ctttttgcag gcggcgcgcg cctggcgcga gaccggcgcg gaaattcttt ttgccgaatt
     3481 aaacaggcag attttgccgg acggcgttta tttcgagcaa tcgacctggt atgcccgcta
     3541 cacggcggat ttttacacgc attttctgat tttaaaagcc ttaagcgaag aggaaagcga
     3601 ccgcgaaacg gaagaaaaac tcgccggaaa acttcagctt ttgctcaatt ttttgatgca
     3661 ggtgacgcga cccgacggaa cgacgccgct catcggcgac gacgacggcg gacgcagttt
     3721 gcctttcggc tacaatcata gcgcgccgaa tgattttcgc gccgttctgt cgacgggcgc
     3781 ggtgattttc gagcgcggcg attataaatt tgtcgcccgc gaggcggctg aggaaacgct
     3841 ctggcttttg ggttccgaag gcatcgacgg gtttgaagat ttgggcgagc tgcgcccgat
     3901 acacaattca aaatcttttc cggcgggcgg ttatttcgtg atgcgcgacg gctgggacac
     3961 gaccgacaat tattttttaa tcgactgcgg aaacgtcggc gcaatgagcg gcgggcacgg
     4021 tcacgccgat acgctcgcct ttgatttggc gtccggcggc agaacgcttt tgattgacgc
     4081 gggaacttat acttatcacg aatcggaaga tttaagaaat cattttcgct caaccgccgc
     4141 gcacaatgct ttgacgattg acaacgaatc gtcgtcgcag acgggcggaa aattcggctg
     4201 gcggacgatg gcaaaaccgc aggtcaacgc gtggattgcc gaaaaccgtt ttgatttttt
     4261 tgagggcgag cacgacggct atcgccattt gcccgaagcg cccgcgacgc acgcgcgcag
     4321 cgttctgttt ttgaaaaacg attactggat aatgcgcgat tttgtcgaga cgaaaggcgc
     4381 gcacgattat caattgaatt ttcatttcaa tccgcagacc gctccgaaaa tcgtcggcgg
     4441 ggaaaacggc agtttttatg tcgacgagga aacggataaa aactccggct tgcgcatcgg
     4501 catcatcggc gataacggcg gctggcagat gagcgaaaac tggatttccg attgctacgg
     4561 caggcgcgcc ggcgcgccgt ttttgcggtt tcaatcgaaa ggcgcggcgg cgcaggaatt
     4621 ttttacgttc ctgtttccga ttgattcgct ttcggaaaag cccgaaattt ttgaaacgga
     4681 aattgcgggc ggtcgcgcct tcgtcgtcaa atttcgcggc tacacggata ttttcgttta
     4741 cgcggacggc gaccggatcg tccgcaccga atttttcgat acggattttc gctttgtctg
     4801 ggcgcgtctg agcgagggcg aagatatgcc ggaagaattc gttttgctcg acggcaaaaa
     4861 ttttgtcgtc aacagccgcg aaatcgtcaa ttacccgcag cgcttaaaat tcgccgcggc
     4921 gcgccgtctg ggcagcaaat taaatgtcaa aacgagcgaa agtcttttca gcgtatcgct
     4981 tcccgcgcgc cgctcgcaca gatatacatt gaaaaattcg gagcaatcgg agtaaacttc
     5041 taaaggcgca tccggctgcc gcatcggatt taaattccgg gctttttttt acgacatatt
     5101 ttcctgaaac gctttagagc gcggaattta taatttacag acgttttaat tcccgacgct
     5161 tcttcctttc tttccgcacg ccagtgaaaa aaaatattct ccaactcgtc ggcagttttc
     5221 attcgggcgg ctccgagcgt caagccgcgc agcttatccg gcttttgcag gaagataaaa
     5281 cgcaccggat tttcgtcgcc tgtttaaacg gcgcgggcgt tttgcgcggc agcgtggaaa
     5341 aactcggttt cgcggaaatt cccgaattca ggctgacctc gttttatgac gcgaatatgg
     5401 ctcgacagct ttcgcgctgc cgcgcattct tacaggaaaa ccgcatcgac attatccaga
     5461 cgcacgattt ttacacgaac gttttcggga tgaccgccgg acgaatggcg cgcgttccgg
     5521 tgcgcgtcgc cgccaagcgc gagacgggaa cgaaatcgag cgcgcagaga ttcgtcgagc
     5581 gccgcgctta cgcttttgct cacgccgtcg tcgccaatgc ctcagccgtc aaaaatcatt
     5641 tgattgaaac gggcgtggcg gcgaaaaaaa tcgaagtcgt ttacaacggt ctggaattgg
     5701 cgaaattcga ttcgaaaaac gagccgcgcg cggaaatttt gaaaagcttc ggtctgccgc
     5761 cggatgaaaa tttgaaattc gtcacgattg tcgccaattt tcgcagcgac gtgaaaaatc
     5821 acccgatgtt tctgcgcgcc gcccgaagcg tcaaagaaaa ttttccggcg gcgcgttttc
     5881 tgctggcggg cgaaggcgaa ttgctcattg aaatgaaaga tttggcgaaa gatttgggat
     5941 tggaaaacga atcgttcttt atagataatt gcggcgacgt gccgcgattg ctgctggcgt
     6001 ccgacatttg cgttttaagc tcccggacgg aaggcttttc caattcgatt ctggaatata
     6061 tggcggctgg aaagcccgtc gtggcaacgg atgtcggcgg cgccggcgaa gcgattgacg
     6121 aaggcgaaaa cggttttctc gtcgagtcga acgacgatgc gtcgatggcg aatcggctgc
     6181 tcgagctttt gcgggacgcc tcaaaggcag aaatattcgg cgcgcgcggg cgcgcaatcg
     6241 ccgaagcgaa gttttcgacg gcggcgcagc tgaagaaaac tttggatttg tatgatagat
     6301 tgttgcagaa cggggaaagg aaatgatttg ccgcagggaa aacgatgaat acggacggtt
     6361 gaacgatgaa ttgcaatttc gcgtttatcg cttgccattt cgtcgtcctg atggcgaatg
     6421 tctcatttgc agatgaatca atcgagcgaa aaaaccaaaa ttctcatcat cgctccggcg
     6481 ctgccgctgg tcggcgggca agccgtgcag gcgcaaaggc ttttggagaa tttcaggcgc
     6541 gagccgcatc tcgaagtcga tttacagccg ataaacccgc aggtcgcgcc gcgcctgcaa
     6601 aaaattccgt acgtgcggac ggcggtcacg tccttgaaat atcttttcga tttattcgga
     6661 aaaattccgc gcgccgacgt cgtgcatatt ttttcggctg cctattacgg ttttgtttta
     6721 tcggtcgtgc ccgcgatttt gctggcaaag cttttcggca aaaaaacgat tatcaattac
     6781 cggagcggcg aggcggagaa tttctttcgc ggctggggcg gatatttttt gtgggcggtc
     6841 agaatggcgg acgcggtcgt ttcgccgtcc ggttatctgg ttgacgtgtt cgagcagttc
     6901 ggcgtcaggg gacgctcgat tttcaatatc atcgacaccg ataaattcac ttttcgcgcg
     6961 cggcgagctt tgcgtccaat ctttttatca aaccgcattc tggaatcgct ctacaacatc
     7021 gaatgcattt ttcgagcttt ccggactgtc agtgaaaaat ttcccgacgc cagactcatc
     7081 gtcgccaatg acggcatcga gcgcgaaaaa ctggaacgcg cggcgcgcga aatgaatttg
     7141 aaaaatgtcg aattcatcgg tctggtgccg caggacaaaa tcgccgcctt gtatgattcg
     7201 gcggacgttt atctgaactc gccgaacttt gattgtatgc ccggttcgct gattgaatgt
     7261 ttcgcctcgg gcttgccggt cgtatcgacc aatgcgggcg gcattccgta tatcgtcgcc
     7321 gacggcgaaa ccggcttgtt agtcgagacg aacgacgacg ctgcgctggc ggcaaaagcg
     7381 atttatcttt tggaaaaccc ggaagccgcg caggcgctta tcgacaacgc gcggaaagaa
     7441 tgcgcgaaat attcgtgggc ggcgattcgc ccgcaatggc ttgcgcttta cgaaaaactc
     7501 gcggctgaat taaagggaaa ccctgagccg cagaagctcc tgg
//
LOCUS       DASWNE010000412         4273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_190343, whole genome shotgun sequence.
ACCESSION   DASWNE010000412 DASWNE010000000
VERSION     DASWNE010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190343"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VGB68_08275"
     CDS             <1..493
                     /locus_tag="VGB68_08275"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY0049264.1"
                     /translation="FALPASLRETSLKMNFVKESIIRAAPEKVFAFHLLPDAFERLIP
                     PWESVRVLQKADISKIGSRTIIESKLFGFFKVKWVAEHTRYEPPRMFEDLQISGPFKS
                     WRHRHIVLPHAEGAVLKDEIEFEPPGSVFGKSLAPVLVLSRLEKMFAHRHEVTRKWCE
                     SEN"
     gene            497..787
                     /locus_tag="VGB68_08280"
     CDS             497..787
                     /locus_tag="VGB68_08280"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HEY0049265.1"
                     /translation="MLIHIVCWKYKPEITEAERAEHRAKLKSLPDVIPDILSFKVGAD
                     ILHLERSFDTGLAAAYPDRAALDAYTAHPSHQEVAALGKQIAEKVVSVDFLD"
     gene            926..2110
                     /locus_tag="VGB68_08285"
     CDS             926..2110
                     /locus_tag="VGB68_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049266.1"
                     /translation="MKRWKKILLVFVAVILTAQIPFCYRRCRIGQLAGKIDALQAARN
                     QSENPQYNDYKGIIHAHTSVGGHSDGTFDELVRAARANHLDYVLMTEHTAELFDTSAL
                     TLNGNYNGTLFVNGQEVNTATGDRFLLITGSGESFRDAKLETPPFIEKYKARNKLVFV
                     TYPEKLKSWDADFDGIEVFSLHTNAKRMNPVLFAEDALWSYYSYPELLLTKYFTRPDE
                     NLRRYDELTGNKKLTLFAGSDAHSNIGFHLLGDDAGNKIINLKFDDYATVFRLARNHV
                     LLEKDKPLTPENLLQALKNGHSFVGFDTLGDTKGFAFTAGDKIMGDEITLADKVDLKA
                     AAPLAARFVIFRNGEKVYEQTATDINFEAREKGAYRVEAYLDSIGFDRMPWIISNPIY
                     VR"
     gene            2448..>4273
                     /locus_tag="VGB68_08290"
     CDS             2448..>4273
                     /locus_tag="VGB68_08290"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY0049267.1"
                     /translation="MQSKSILKLFVLAVSFTFLLTQNVFAQATGSLSGTVKDPNEAVV
                     QGATVTVKNSATNLTRTTMSDEEGRWTIQVLPVGNYSVTYEKEGFKKSLSENVAVEAS
                     VPRTVEVSLEVGSTGAVVTITSDQPLVQLETSAVSRQITGEQLTKTPTSTRSFTGLLS
                     SEAGVSSELSPVGVNGNGNVSPSVNGTRTTSTSLTFNGIDATNITSNEGSLNDNISPA
                     PETLQEVKLQTSLYDASTGRSGGGNFQLVTKSGGNRFTGSAYYYLQNKRFNSNEFFLE
                     RAGEEKPRADRTETGFTIGGPVIKDKFFFFGGYQYTQANTGLVPTARTRTVLPLALTV
                     LGDDRSAAAIAAAFNQFNGCAGANCLTAGDISPVALRIFNLRNPVTGGFVLPSLQGRN
                     YRALTTTQIVNGQPATVALTDQTGTGSYQFGSFPGVTTRTLQRNNPLVEFIDVEPSRF
                     KQQQFTTRLDGQLSQKNTLSGTLFYSDFPGFDSFPDPNSLASPFTLRRNDRNRTLAIS
                     DTHVFSSNLINEVRFGYFYLNNSRRLDDPFLELTNESIGVFNPATVFDDSIATTRLGH
                     YIGRNNIANFSFGGPNDSFNKRKQITYSIGDNVNYISGNHNI"
BASE COUNT         1072 a         1162 c         1066 g          973 t
ORIGIN      
        1 ctttgcgctc ccggcgtctt tgcgggaaac ttctcttaaa atgaatttcg tcaaagaatc
       61 aatcatcaga gccgcgcccg aaaaagtttt tgcttttcac cttctgcccg acgccttcga
      121 gcgcctgatt ccgccgtggg aaagcgtcag ggttttgcaa aaagccgata tttccaaaat
      181 cggctcgcgg acgataatcg aatcgaaatt gttcggattt tttaaagtca aatgggtcgc
      241 cgaacacacg cgttacgagc cgccgcgaat gtttgaagac ttgcagattt ccggaccttt
      301 caaaagctgg cgacaccggc atatcgtttt gccgcacgcg gaaggcgcgg ttttaaaaga
      361 cgaaatcgaa ttcgagccgc ccggttcagt gttcggaaaa tcgctcgcgc ccgttctggt
      421 tttgtccaga ctggaaaaaa tgttcgccca tcgccacgaa gttacgcgaa aatggtgcga
      481 atccgaaaat taatttatgc tgatacatat tgtttgctgg aagtataagc ccgaaattac
      541 cgaagccgag cgcgccgaac atcgcgcgaa gctgaaaagt ctgccggacg taattcccga
      601 tattttgagt tttaaggtcg gcgcggacat tctgcatctc gaacgctctt tcgataccgg
      661 cttggcggcg gcttatcccg accgcgcggc gctggacgct tacaccgcgc atccgagtca
      721 tcaggaagtc gcggcgctcg gcaaacaaat cgcggaaaag gtcgtttcgg tcgatttctt
      781 agattgagtt gaccgagttg actgaaagtt gactgagttg actgaaagtt gactgagttg
      841 cccgagttac tatgaacttc gcgagcttaa caaactcagt caactttcta aaactttcag
      901 tcaactttca gtcaacttat aaaatatgaa gcgctggaaa aaaattctgc tcgttttcgt
      961 cgccgtcatt ttgacggcgc agattccgtt ttgctaccgg cgctgccgaa tcgggcagct
     1021 ggctgggaaa atcgacgcgc ttcaagccgc gagaaatcaa tcggaaaatc cgcaatacaa
     1081 tgattacaaa ggcattattc acgcgcacac aagcgtcggc gggcacagcg acggaacttt
     1141 cgacgagctc gttcgagccg cgcgggcgaa ccatctggat tatgtcctga tgaccgagca
     1201 caccgccgag cttttcgaca cttcggcttt aaccttaaac ggcaattaca acggcacttt
     1261 gtttgtcaac ggtcaggaag tcaacacggc gacgggcgat agatttttat tgattaccgg
     1321 cagcggcgaa agctttcggg acgccaagct cgaaacgccg ccgtttatcg aaaaatacaa
     1381 agcgcggaat aagctcgttt tcgtcaccta tcccgaaaaa ttaaaatcct gggacgccga
     1441 ctttgacggc atcgaggttt tcagcctgca cacgaacgcc aagcggatga atcccgtgct
     1501 gtttgccgaa gacgcgctct ggtcttatta cagctatccg gaactgcttc tgacaaaata
     1561 ttttacgcgc cccgacgaaa atctgcgccg ctacgacgaa ttgaccggaa acaaaaaatt
     1621 aacgcttttc gccggaagcg acgcgcattc gaacatcggt tttcacctgc tgggcgacga
     1681 cgcgggcaat aaaatcatca atctgaaatt cgacgattac gcgaccgttt tccgcctcgc
     1741 cagaaatcac gttctgctcg aaaaagataa gccgctgaca ccggaaaatc ttttgcaggc
     1801 tttaaaaaac ggtcactcgt tcgtcggctt tgatacgctc ggcgatacaa aaggcttcgc
     1861 tttcacggcg ggcgataaaa ttatgggcga cgaaatcacg ctcgccgata aagttgattt
     1921 gaaagccgcc gcgccgctcg ccgctcgttt cgtcatcttc cgaaacggcg aaaaagttta
     1981 cgaacagacc gcgacggaca tcaattttga agcgcgcgaa aaaggcgcgt atcgcgtcga
     2041 agcttatctg gactcaatcg gtttcgaccg gatgccctgg attatctcaa atccgattta
     2101 cgttagatag ttgacaggaa gttgcagata gttgcagata gttagaaaca ggttcagcgt
     2161 ttcaactttc ggcaaccgtc ttaactttcc gcaactctgc aacttcctgc aactctgcaa
     2221 cttcctgtca actatccgca acttcccgca actccataaa aaataattaa cattttttta
     2281 acaccgcgtt catttgcctg taatttgccg gtcatattct aacgcttgca gcattctatt
     2341 tacattttca aaaaaaaact tcgagaaaag gacagcggca agcgggcttt caggcgacag
     2401 gtctatcaag ccgcgaattg ctttttaagt tcatccagga gaaaccgatg caatccaaat
     2461 ctattttgaa actttttgtt ttagccgtca gttttacatt tttattaacc cagaacgttt
     2521 ttgcacaggc gacgggaagt ttgagcggaa cggtgaaaga cccgaacgag gcggtcgtgc
     2581 agggcgcaac cgtaacggtt aaaaattccg ccaccaattt gacacgcacg acgatgagcg
     2641 acgaggaagg acgctggacg atacaggttt tgcccgtcgg caattacagc gtgacatacg
     2701 aaaaggaagg cttcaaaaaa agcctgagcg aaaacgtcgc cgtcgaggct tccgttccgc
     2761 gcacggtcga agtttcgctg gaagtcggct cgacgggcgc ggtcgtaacc atcaccagcg
     2821 accagccgct cgtgcagctc gaaacatctg ccgtgtcgcg ccagattacg ggcgagcaat
     2881 taaccaaaac cccgacctcg acgcgcagtt ttaccggact tttgtcgtcg gaagcgggcg
     2941 tcagctcgga gctttcgccg gtcggcgtca acggcaacgg aaacgtttcg ccgtcggtca
     3001 acgggacgcg cacgacttcg accagtctta cttttaacgg catcgacgcg acgaatatta
     3061 ccagcaacga aggttccttg aacgacaata tctcgcccgc gccggagacc ttgcaggaag
     3121 tcaagctgca aacgtcgctc tacgacgctt cgaccggcag aagcggcggc ggaaattttc
     3181 agctcgtcac caaatcgggc ggcaaccggt ttaccggctc ggcttattat tacctgcaaa
     3241 acaaacgctt taactcgaac gaattttttc tggaaagagc cggcgaggaa aaaccgagag
     3301 ccgaccgcac ggagaccgga tttaccatag gcggacccgt tatcaaagat aaattcttct
     3361 ttttcggcgg ttatcaatat actcaagcca atacgggctt ggttccgacg gcgcggacgc
     3421 ggacggtgct gccgctggcg ctgaccgttt tgggcgacga ccgctcggcg gcggcgattg
     3481 cggcggcatt caatcaattc aacggctgcg cgggagcaaa ctgcctgacg gcgggcgaca
     3541 tcagcccggt cgcgctgcgc attttcaatt tgcgcaaccc ggtgacgggc ggtttcgtct
     3601 tgccgagcct gcagggcaga aattatcgcg cgctgacgac gacgcaaatc gtcaacggtc
     3661 agccggcgac ggtcgcgctg accgaccaga cgggaacggg cagctaccaa ttcggttctt
     3721 ttccgggcgt gacgacgcgc actctgcagc gaaacaatcc gctggtcgaa tttatcgacg
     3781 tcgagccgtc gcgcttcaag cagcagcaat tcacgacgcg cctcgacggg cagctgagcc
     3841 agaaaaacac tctgagcggg actttatttt actcggattt tcccggcttc gattctttcc
     3901 ccgacccgaa ctcgctggct tcgccgttta ctctgcgccg caacgaccgc aaccgcacgc
     3961 tggcaatttc ggacacgcac gttttttctt caaatctgat taacgaagtt cgcttcggct
     4021 atttctatct gaacaattcg cgccgcctcg acgacccgtt tctcgaactg accaacgaat
     4081 caatcggcgt tttcaatccg gcgaccgtct ttgacgacag catcgccacg acgcgtttgg
     4141 gtcattacat cgggcgcaac aacatcgcca atttctcgtt cggcggtccg aacgattcgt
     4201 ttaacaagcg caagcaaatc acttacagca tcggcgacaa cgtcaattac atttccggca
     4261 atcacaacat caa
//
LOCUS       DASWNE010000413         7811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_261619, whole genome shotgun sequence.
ACCESSION   DASWNE010000413 DASWNE010000000
VERSION     DASWNE010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7811
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261619"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="VGB68_08295"
     CDS             complement(<1..892)
                     /locus_tag="VGB68_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049268.1"
                     /translation="MNYPIFTIKAHRNFIKIAAACFLILTAAGNLLAQYQGAPVKKDR
                     LIQALRSRQLQTGDIVTIINRNGVDFELTPAVRKSLIAAGARPEVIKAVSNNPRLLSK
                     SDNITAKNGKINRIRRKNPAPANYDDLLEQAIYSYKDQKNPKAAVRILENAAKINPAS
                     PAAYQMLGFIYLYELNNLAQAEKSMREAFINGGSAVFRVFHDDNGKFTGRCTGSLYIS
                     QDSLRFESDENVHTFETSIANVDKIKLDTETTRIWKKRSIFKIFLKIGKEEAKFRFAP
                     LTGVQEESKMVERFIAATNSN"
     gene            complement(938..1408)
                     /locus_tag="VGB68_08300"
     CDS             complement(938..1408)
                     /locus_tag="VGB68_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049269.1"
                     /translation="MKIFNRNERRASGAAGESNVRRLPLNKAPQRHYTRAGAAIFVII
                     ILHFVSQFIFFQSEKNAPPTEAVNHQSVEVEPEAEIKPEIEIKPENNRRDEVKMESAA
                     KKPELTTRRPNAIQPIVQPEPTGAPSRVVIKKKEPRVESRAERLRRAERLLTGV"
     gene            1684..2151
                     /locus_tag="VGB68_08305"
     CDS             1684..2151
                     /locus_tag="VGB68_08305"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY0049270.1"
                     /translation="MNEEKSLQETFAPHGICFGCGIENEKGLRIRSFPSGAEVVCEWR
                     AEPHHQAFPGMLSGGIVGTLLDCHSNWTAAYHLMKKNGKSEPDCTVTANYSVKLLRPT
                     PFDATIKLTARVVESSKDRATIEAELIAGDKVCATCKGLFVVVKEGHPAYHRW"
     gene            complement(2172..2744)
                     /locus_tag="VGB68_08310"
     CDS             complement(2172..2744)
                     /locus_tag="VGB68_08310"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049271.1"
                     /translation="MKILVARSANEIETAFTKLSAAAEIGCDTETSSLSARYGKLFSV
                     QFSDGDFNVLVPISEGVALGRLAQILENPSITKIFHNAKFDLEFLRENGYQAQNVFDT
                     MIAEKVLTKGADQSASLAETLYRYFAVDLEKSHRHAFTKNWNGVWTDELVHYALSDVV
                     YLPELKKQQQLWLKRLGLKEDFARQMKKII"
     gene            2897..3544
                     /locus_tag="VGB68_08315"
     CDS             2897..3544
                     /locus_tag="VGB68_08315"
                     /inference="COORDINATES: protein motif:HMM:NF016240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HEY0049272.1"
                     /translation="MSEKLTETEARVVGALIEKQLTTPEYYPLTLNALVAACNQKTNR
                     EPVVAYDEETVTRALDNLRERNLVYVFYGSTSRVPKYKHMLPDVYELEPSETAVLCVL
                     LLRGAQTLGELRERTGRLYEFRGLGDVNETLDALVRRDEPLIVKLERQPGQKEARYAH
                     LLSGEVASYQPPEKSSSRASAENERIENLERELENLRSEFNLFRQEFEDFKKQFE"
     gene            3622..3975
                     /locus_tag="VGB68_08320"
     CDS             3622..3975
                     /locus_tag="VGB68_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049273.1"
                     /translation="MGYIFVFLFIAIALIIILYYFFRTRSDTGSIGINKQNQPSVIQG
                     SDSADSAVKRLPDNDSELMRLRQHLNTIVLHNKRLYQNNLDIARRDFTKRGLNNPSET
                     ELLKRAIEIWQDDNR"
     gene            complement(4043..5743)
                     /locus_tag="VGB68_08325"
     CDS             complement(4043..5743)
                     /locus_tag="VGB68_08325"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HEY0049274.1"
                     /translation="MKIKKRFFFRAFCSFVFLFSASLVNAAVYEVKPNTALDTIAEVP
                     WATLQPGDTVLIYWRAAAYKEKWVICRQGTASAPITVRGVPNQNGDLPVIDGSGAVTP
                     SNLNFAGEQRGVIKIGSANIPADTMPKYITIENLEIRGAHPDYQFADDGGNVQTYSSA
                     ASSIYVEKGENITVRSCKIHDSANGFFVASSDAAVSRNILVEGNYIFGNGIAGSAFQH
                     NNYTAAIGITFQYNRFGAMRAGAGGNALKDRSAGTVVRYNWIEHGNRQLDLVEGDDSV
                     QIRNAPEYRKTYVYGNVLIEPDGAGNSQITHYGGDNGNAALYRKGTLYFYNNTIVSTR
                     TGNTTLFRLSTNEENCDARNNIFYVTAGGANLALLDDTGVLRLSHNWLKPNWRISHGT
                     PTGTVNNDGTTVQGASPGFVSEAAQDFRLVSTAAAINAGTILNTDVLPPHNVIRQYVK
                     HQTSEARPANGQFDIGAYEFGGAPMQITTDSLPNARRGKFYNQTLQASGGSGAFLWTI
                     SAGNLPPGMWLDSATGKIYGKAALKGVWNFTVTAQDSQNPAATTSRNFTINVRWYSGF
                     "
     gene            complement(6097..7020)
                     /locus_tag="VGB68_08330"
     CDS             complement(6097..7020)
                     /locus_tag="VGB68_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049275.1"
                     /translation="MSKKFQALLRSVFSFAFIVLVFGSYVIVAQTTTLTGDWKADARS
                     EKHPEKIQLSFTRRSEKGGTNQHGSSFEYADLQGLSREQALGSGAVKFSLVREAGTIE
                     CEGRFDGTGKGLGTFRFTGNQQFLSAMKSRGFDFEAAEAGKKSRDLDERLFAAAMINV
                     TTAFTDDLLSANFGKLTVDDLFKAAIFKITPQFMSEMKASGFPNLGMEELVKARIFKI
                     DANFVREVTQMGFEKEPFETLVKMRIFKVTPEFITEVRNEGLTNLDVEDLVKMRIFKI
                     DGAFIRQAKSEGVPLEVEKLVQKRIGVYGRD"
     gene            complement(7116..>7811)
                     /locus_tag="VGB68_08335"
     CDS             complement(7116..>7811)
                     /locus_tag="VGB68_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049276.1"
                     /translation="MSIKQVISDTFRLLTFRVAREEILNFGWSHFAFGLISAWLVGIG
                     RYWDNPKAEFLQQLGVGSLLYIFALSLLLWFVVKPLRPQDWSYFYVATFVALVSPPAI
                     LYAIPVQMFFDIGAANEINAVFLLIVSVWRLALLVFFLKRFAALNPGYVLVSTLLPIT
                     MIIFALVMLNLEKAVFSIMGGFSDRTPNDEAFMFLNLISLFAMLAFPVLLISYIVIIV
                     AELSGKRKQKISA"
BASE COUNT         1966 a         1935 c         1905 g         2005 t
ORIGIN      
        1 tatttgaatt cgtcgcggcg ataaagcgtt cgaccatttt cgattcttcc tgtacgccgg
       61 tcagcggcgc gaatctgaat ttggcttctt ccttgccgat tttgagaaag attttaaaga
      121 tgctgcgttt tttccagatg cgcgtggttt cggtgtctaa tttgatttta tcgacgttcg
      181 ctatcgaagt ttcaaaagta tgcacgttct cgtccgactc gaaacgcaag ctgtcctgag
      241 aaatatacag cgaaccggtg cagcgcccgg taaattttcc gttatcgtcg tggaaaacgc
      301 ggaaaaccgc gctgccgcca ttgataaagg cttcgcgcat agatttttca gcctgagcga
      361 gattgttcag ctcataaaga taaataaaac cgagcatctg atacgccgcc ggactcgccg
      421 gatttatttt ggcggcattt tccaggatac gcaccgcggc tttcggattt ttctggtctt
      481 tataggaata aatcgcctgc tcgagcaaat cgtcgtaatt tgccggcgcg ggatttttcc
      541 ggcggatgcg gttaattttt ccgtttttcg ccgttatatt gtcgcttttt gacagcaggc
      601 gcggattatt agacacggct ttaattactt cggggcgcgc gccggcggca atcagacttt
      661 ttctgactgc cggagtcagc tcgaaatcaa ctccgttgcg gttgataata gtgacgatgt
      721 cgccggtctg caattgtctc gaacgaagcg cctgtataag ccggtctttt ttaaccggcg
      781 cgccttgata ctgcgccaat aaattaccgg cggctgttaa aattaaaaaa cacgctgccg
      841 caattttgat aaaatttctg tgagccttga ttgtaaaaat cggatagttc attattttta
      901 caatttcctt tcccgcttga ataaaattta tggaaatcta aactcccgtt aaaagccttt
      961 cggcgcgacg caggcgttcc gctctggatt cgacgcgcgg ctcctttttc tttatgacga
     1021 ctcgtgaggg cgcgccggtg ggttcaggct ggacaatcgg ctgaatggcg ttcggtcgtc
     1081 tggttgtaag ctccggtttt ttcgcggcgg attccatttt gacttcgtcg cgccggttat
     1141 tttcaggttt gatttcaatc tccggcttga tttcggcttc aggctcgact tcgacgcttt
     1201 gatgattgac tgcttcggtc ggcggagcgt ttttttcact ctgaaaaaag ataaactgtg
     1261 aaacgaaatg tagaataatg ataacaaaaa ttgccgcgcc cgccctggta taatgccgct
     1321 gcggagcttt atttaaaggc agccttcgga cattcgattc tccggctgcg ccgctcgccc
     1381 gtctttcatt tcgattaaat attttcatca cttaaagctt tttttgatgc gttcaccggc
     1441 agatgagttg cagcaaagaa aaacatcgtt aaacacaagt ccggattttt gcggacgatt
     1501 tccgctggcg gtgaaattat atttttttta agcgtcacaa aatctttagt ccggtcagcc
     1561 gactctttaa gtataacacg aacttatcgc ctgcaaataa ctaatcttta aacatttttg
     1621 tttttatcgg cgagcgaaaa tcgatgggtt tttggcagaa gaaaattgag ctattataaa
     1681 actatgaacg aagaaaaatc ccttcaggaa acttttgcgc cgcacggaat atgtttcggc
     1741 tgcggcatcg aaaacgaaaa aggcttgcgc atccgcagtt ttccgagcgg cgcggaagtc
     1801 gtctgcgaat ggcgcgccga gccgcatcat caggcgtttc ccggaatgct ctccggcggg
     1861 attgtgggaa ctctgctcga ttgtcattcc aattggacgg cggcttatca tctgatgaaa
     1921 aagaacggaa aatccgagcc cgactgcacc gtaacggcaa attattcggt taaactgctg
     1981 cgcccgacgc cttttgacgc gacgataaaa ttaacggcgc gcgtcgtcga atcgagcaaa
     2041 gaccgcgcga caatcgaagc cgaactaatt gcaggcgata aagtctgcgc gacctgcaaa
     2101 ggcttgttcg tcgtcgtcaa agaaggtcat ccggcgtatc atcgctggtg agatttcgcg
     2161 ctttttcgcg gctaaataat ctttttcatc tgtctggcga aatcttcctt caagcccaga
     2221 cgtttcagcc agagctgctg ttgttttttt aattcgggca gataaaccac gtccgagagc
     2281 gcgtaatgga cgagctcgtc cgtccagacg ccgttccagt ttttggtaaa cgcgtggcgg
     2341 tgagattttt ccaaatcgac ggcgaaatag cgataaagcg tctccgccag cgaagccgat
     2401 tggtcggcgc ctttggtcag aaccttttcg gcaatcatcg tgtcgaaaac gttctgcgcc
     2461 tgatagccgt tttcgcgcag aaattctagg tcgaatttcg cgttatgaaa aattttcgta
     2521 atcgacgggt tttctaaaat ttgggcgagc cgaccgagcg cgacgccttc ggaaatcggc
     2581 actaaaacat taaaatcgcc gtcggaaaac tgcactgaaa acagctttcc gtagcgcgcg
     2641 cttaaagaag atgtctccgt atcgcagccg atttccgccg ccgccgagag tttggtaaaa
     2701 gccgtttcga tttcatttgc cgagcgcgcc acgagtatct tcacacttcg attgtaaatt
     2761 aataaataaa caactgagaa atgacgcggc gagtcgagcc tgcaatttga aatccgggat
     2821 ttgaaatttc aaattataag cttcgcctgc cgcgtttaca gttctccgat ttttgcttta
     2881 tgctgttaaa ccggatatgt ctgaaaaatt aaccgaaacg gaagcccgcg tcgtcggcgc
     2941 gctaatcgaa aaacaactga cgacgcccga atattatcct ttgacgctaa atgcgctggt
     3001 cgccgcctgc aaccagaaaa cgaaccgcga gccggtcgtc gcttacgacg aggaaaccgt
     3061 cacgcgcgcg ctggacaatc tgcgcgagcg aaatctcgtc tatgtttttt acggcagcac
     3121 gagccgcgtg ccgaaatata aacatatgct gccggacgtt tacgagctcg aaccgtcgga
     3181 aaccgccgtt ctctgcgttc ttttgctcag gggcgcgcag actttaggcg agctgcgcga
     3241 gcgcaccggc aggctttacg aatttcgcgg tctcggcgac gtcaacgaaa cgctcgacgc
     3301 gcttgtcaga cgcgacgagc cgctgatcgt caaactggag cgccagcccg gacaaaaaga
     3361 agcccgatac gctcatcttc tgtcgggaga agtcgcttcg tatcaaccgc cggaaaaatc
     3421 gtcttcacgc gcttcggcgg aaaacgaaag aatagaaaat ctcgagcgcg agctggaaaa
     3481 tctgcggagc gaatttaatt tgttccggca ggaatttgaa gactttaaaa aacaatttga
     3541 ataagaatat tgcagctatg tcgcggcagt tttattgtag ctcccgcttt ttatttaatt
     3601 taataaagga gtaattgtct catgggctat atttttgttt ttttgtttat agcgattgcg
     3661 ttaattatta ttctctacta ctttttccga acgcgttccg ataccggctc gattggaata
     3721 aataaacaaa accagccgag cgtcatacaa ggttctgatt cagccgatag tgcagtaaag
     3781 cgactgcctg ataatgattc tgaattaatg cgactcaggc agcatctaaa tactattgtt
     3841 ctgcacaaca agcgtttata tcaaaataat cttgacattg cccgccgcga ttttacgaaa
     3901 agaggattga ataatccttc agaaacggaa ttactaaaac gggctattga aatttggcag
     3961 gacgataaca gatagacgcc gaatttcatt ttattcgaac ctttaacgat ttttaaacga
     4021 taaggcttat acgaatcttt aatcaaaaac ctgaatacca tctcacgtta atcgtaaaat
     4081 tcctgctcgt tgtcgccgcc ggattctggc tgtcctgagc cgtcacggta aaattccaga
     4141 cgcctttgag cgcggctttc ccgtaaattt ttcccgtcgc cgaatcgagc cacattccgg
     4201 gcggaagatt tcccgccgaa attgtccata aaaacgcgcc cgaaccgccc gaagcctgca
     4261 aagtctgatt ataaaatttg ccgcgccggg cgttcggcaa gctgtcagtc gtgatttgca
     4321 tgggcgcgcc gccgaattca tatgcgccga tgtcgaattg accgttcgcg ggacgcgctt
     4381 cgctcgtttg atgtttgacg tattgacgaa tgacgttatg cggcggcaaa acgtccgtat
     4441 ttaaaattgt tccggcgttg attgccgccg cagtcgaaac gagacgaaaa tcctgcgccg
     4501 cttcgctgac aaatccgggc gaagctccct gaaccgtagt tccgtcgttg ttgaccgttc
     4561 cggtcggcgt cccgtgcgag atgcgccagt tcggcttaag ccaattgtgc gaaagccgga
     4621 gaacgcccgt gtcgtcgagc aaagccagat ttgcgccgcc cgccgtcaca taaaaaatat
     4681 tgtttcgcgc gtcgcagttt tcctcgttgg tcgaaagacg aaaaagcgtc gtgtttccgg
     4741 ttctggtcga aacgattgtg ttgttgtaaa aataaagcgt gcctttgcgg taaagcgccg
     4801 cgttgccgtt gtcgccgccg taatgcgtga tttgcgaatt gcccgcgccg tccggctcga
     4861 tcaaaacgtt gccgtaaacg taagttttgc gatattcggg cgcgttgcga atctgcaccg
     4921 agtcgtcgcc ttcgaccaaa tccagttggc gatttccgtg ttcaatccag ttgtatcgaa
     4981 cgacggttcc cgccgagcgg tctttcagag cgttgccgcc agcgcccgcg cgcatcgcgc
     5041 cgaaacggtt gtattgaaac gtgatgccga ttgccgccgt gtaattgttg tgctgaaacg
     5101 cgctgcccgc gatgccgttg ccgaaaatat aattgccctc gaccagaatg tttcgggaaa
     5161 ccgcggcgtc gcttgaggcg acaaaaaatc cgttggcgct gtcgtggatt ttgcagctgc
     5221 gaacggtgat gttttctccc ttttcgacgt aaatcgaaga agccgcgctg gaatatgtct
     5281 gcacgtttcc gccgtcgtcg gcgaattgat aatcgggatg cgcgccgcga atttccagat
     5341 tttcgattgt gatgtatttc ggcatcgtgt cggcgggaat attggcgctg ccgattttga
     5401 tgacgccgcg ctgctcgccg gcgaaattca gatttgacgg cgtgaccgcg ccgctgccgt
     5461 caatcaccgg caaatcgccg ttttgattcg gcacgccgcg caccgtaatc ggcgcgctcg
     5521 ccgtgccctg acggcaaatc acccattttt ccttatacgc cgccgcgcgc cagtaaatca
     5581 aaaccgtatc gcccggctgc aaagtcgccc acggaacctc ggcaatcgtg tcgagagccg
     5641 tgttcggctt gacttcataa acagctgcat taacaagact tgcggaaaaa agaaaaacga
     5701 acgaacaaaa cgcgcgaaag aaaaaccgct tctttatttt catattattt caatattgtt
     5761 tgggattttt tacaacaggt ctcgtgagag gttatcgacg gctcggacga aaagttgccg
     5821 acttttaaaa aataaaatca tttaaatttt agcaccggct ccgaagccgc gtctatattt
     5881 tatttggcgg gaaattatga acgccgattc gaatcgccgc gtccgccgac gggatttatt
     5941 attgttttca aagataaaag gagcgaaaaa tatcagtcgc cggaaggcgt ttttacgaga
     6001 aacgccgagt aaaataaaag agacacgccc gttaaaccgg cgtgtctctt tctctctctg
     6061 tcagtgtgtg tatctttctg tctgaaataa atcgaattaa tctctgccgt agacgccgat
     6121 gcgtttctgc accagttttt cgacttcgag cgggacgcct tcggatttag cctggcggat
     6181 aaacgcgccg tcgattttaa aaattctcat tttcacgagg tcttcgacat ccagattcgt
     6241 caagccttcg ttgcgaactt cggtgatgaa ttcgggcgtc actttaaaga tgcgcatttt
     6301 gacgagggtt tcgaagggct ctttttcaaa acccatctgc gtgacctcgc ggacgaaatt
     6361 ggcgtcaatc ttaaaaatgc gggctttgac cagttcttcc atcccgagat tcggaaaacc
     6421 gctcgccttc atttccgaca taaactgcgg cgtgattttg aaaatagcgg ctttaaacaa
     6481 atcgtccacg gttaatttgc cgaagtttgc cgacaacaaa tcgtctgtaa aagcggtcgt
     6541 cacattaatc atcgcggcgg cgaaaagcct ttcgtcgagg tctcggcttt tcttgcccgc
     6601 ttcggcggct tcaaaatcga aaccgcgact tttcatcgcc gagagaaact gctgatttcc
     6661 cgtgaagcga aaagtgccca gacctttgcc ggttccgtca aatctgcctt cgcattcaat
     6721 cgttccggct tcgcggacga gactgaattt gaccgcgccg ctgccgagcg cctgctcgcg
     6781 gctcaaaccc tgcaaatcgg cgtattcaaa cgatgagccg tgctgattcg taccgccttt
     6841 ttcgctgcgg cgagtaaaag ataattgaat tttttccgga tgtttttccg acctggcgtc
     6901 ggctttccaa tcgcccgtca aagtcgtcgt ctgcgcgacg atgacgtaag aaccgaacac
     6961 aaggacaata aacgcaaaac taaaaaccga acgcaataga gcctgaaact ttttcgacat
     7021 aaacattctc ctcaaaaggt attattatta aaaaatagat gcggcgcgca ttacataaaa
     7081 gaaacaccgc gcgcgcgagg tttgtgacag aaattttaag cggagatttt ttgctttcgt
     7141 ttgccggaaa gctccgcgac gattatgacg atgtaagaga taagcaaaac gggaaaagcc
     7201 agcatcgcga aaagcgaaat cagatttaga aacataaagg cttcgtcatt cggcgtgcgg
     7261 tcgctaaatc cgcccataat gctgaaaacg gctttttcca gattcagcat caccagcgcg
     7321 aaaataatca tagttatcgg aagcagcgtc gataccagaa cataaccggg attgagcgcg
     7381 gcaaagcgtt tcagaaaaaa caccagcaaa gccagtcgcc agaccgagac aatcagcaga
     7441 aagacggcgt ttatttcatt tgccgcgccg atgtcgaaaa acatctgaac cggaatcgca
     7501 tacaaaatcg cgggcggcga aaccagcgcg acaaatgtcg cgacgtaaaa atacgaccag
     7561 tcttgcgggc gcagcggctt gacgacaaac cacaaaagaa gcgagagagc gaaaatgtag
     7621 agaagcgacc cgacgccgag ctgctgcaaa aattccgcct tcggattgtc ccagtaacgc
     7681 ccgatgccga ccagccacgc cgaaatcaaa ccgaacgcaa aatgcgacca gccgaaattt
     7741 aatatttctt cgcgcgcgac gcgaaaagtc aataaacgaa aagtatcgga aattacttgt
     7801 ttaatcgaca t
//
LOCUS       DASWNE010000414         5458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_326325, whole genome shotgun sequence.
ACCESSION   DASWNE010000414 DASWNE010000000
VERSION     DASWNE010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5458
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326325"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..700)
                     /locus_tag="VGB68_08340"
     CDS             complement(161..700)
                     /locus_tag="VGB68_08340"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY0049277.1"
                     /translation="MKTIIRFMLTAAIGFASFVAVAEAQVAGNPPYTLEQSVISSGGG
                     ATAGGAGGVYQIESAIGQPVAGTNSTGGAMFSVRGGFFTPAPFAPTAATASVSGRILT
                     SDGRGIRNVSITMTDASGAMRMAFSGSFGYFTFEDVRVGEVYVLSVRAKQFWFGQSSL
                     AISVREDLSDISFTADPLQ"
     gene            complement(817..2268)
                     /locus_tag="VGB68_08345"
     CDS             complement(817..2268)
                     /locus_tag="VGB68_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049278.1"
                     /translation="MLLIVFSFVLSLCVSAQTTAFTYQGRFTDSSVTQPTNGNYTMTF
                     RLFDAATNGNQIPDNTTQISSLVSVVNGIFTVKLDFGAAAFNTTSARFLEIQVGSTVL
                     TPRQEITSAPFSNRAINSSSADSLSSACVGCVTNAQINSIDGGKVTGTVANANTANTA
                     NTAATAGNVTGVVQIANGGTGSSTQNFVDLSTNQTNIGGNKTFTGLVTVQNAGANSDI
                     ITGVNSAVAGTGGGAGLVGVTNQAGSGAAGVWGQNNNTNGTGIVATGNNQAGNTLVAG
                     SGLAATGFTTAIYGRTNSAGASQVIYSDNFGSITRVNYWNGTTQFKINGTGTVSTLVR
                     DTEQRQRVMFAPEAPEVLFEDYGVGQLVEGRARIVLDPIFAAGITVNDKHPLRVFIQL
                     EGDCNGVYVTNKTADGFEVVELAQGKSNVKFSWHVVGNRADEELGNVRQPDGSVTPSR
                     ISRYTDLRFPVQEVDAPALTAKPDEKTTKPQNK"
     gene            2691..3536
                     /locus_tag="VGB68_08350"
     CDS             2691..3536
                     /locus_tag="VGB68_08350"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY0049279.1"
                     /translation="MDDKRLEEAMKLNDESPEISADEEKLLDQLGLFGESRRQFLGQG
                     IGGGLGIFALQLLAKEQALAALSAPPDAVFAQNAGLENAVRISLKINGAAQSLEVDSR
                     MSLLDTLRERLDLTGTKKGCDQGQCGACTVLVNGSRVLSCLTLAAQCQGKEVTTIEGL
                     AKGDELHPMQAAFIRHDGFQCGYCTPGQICSAVALLNEAKNGDASHVTKNLRTNARNI
                     KLTDEEIRERMSGNICRCGAYPGIVAAIREVQSGRETAQTWHFATDDEIAAVMNEEEK
                     SDEIV"
     gene            3523..4509
                     /locus_tag="VGB68_08355"
     CDS             3523..4509
                     /locus_tag="VGB68_08355"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEY0049280.1"
                     /translation="MKSFKYSSATDAAGAVRAVSANRSAKFLAGGTNLIDLMKEYVER
                     PDELVDVSQLKLAQIRPTSNGVSIGALAKNADTANHPLVRQQYPLLSMAILAGASGQL
                     RNMATNGGNLMQRTRCQYFYDIAMPCNKREPGTGCGALEGLNRIHAIFGWSEKCVATY
                     PGDMANALYALDAVVRVRGAEGRERTIPVGEFHRLPGDTPERDNNLRHGELIVAIDLP
                     KSNFAQNSYYLKVRDRASYAFALVAVAAALELDGGTIKQARVVLGSVAHKPWRSAEAE
                     AALAGRPASEESFRRAADAALKDARPLAHNAYKVELGKRAIVRSLMRASKLA"
     gene            4538..>5458
                     /locus_tag="VGB68_08360"
     CDS             4538..>5458
                     /locus_tag="VGB68_08360"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY0049281.1"
                     /translation="MPRYIGKEMSRVDGVAKVTGKAKYAAEFRAPNLAYGFIVLGTVA
                     KGTIKSIDTREAEGAPGVVRVFTHLNAPKLGPKASHEQAPPRATEEKDKSFRALQSDR
                     IYFNMQPVALVVAETYEQARYAARLVKVAYNAERHMTDTEAVRERARVPSQGPPPKPR
                     GNPEAAMKSAPVRVEAEYRIPVEHHNPMEPHAAVAVWEGDRLTVFDKSQEVYNVRKHL
                     ASSFGVPETNVQVISPYVGGAFGSSLRPNYYPALTAMAAREIKRPVKVVYTRTQMFTG
                     HGYRPHTIQKIALGAERSGKLTAMIHHAVHN"
BASE COUNT         1165 a         1538 c         1647 g         1108 t
ORIGIN      
        1 aaaaaatcaa attttaaatt tcaaagatga cacgaaaatt ccggcttccg aaacgaattt
       61 acgtctgccc gttttgccga aaaagtgttt tattctaata tttcccggac gacgagcgat
      121 tcgtttgacg gattttatta ttcgaattgc tttgacgcgc ttactggagc gggtcggcgg
      181 taaagctgat gtcggacaag tcttcccgga cggaaatcgc cagcgagctt tgaccgaacc
      241 agaattgctt ggcgcggacg ctcaggacat aaacttctcc gacccgcacg tcttcaaacg
      301 taaaatagcc gaaactgccg ctgaacgcca ttcgcatcgc tccgctcgcg tccgtcatcg
      361 ttatcgaaac gttacggatg ccgcgcccgt ccgaggtcag aattctgccg ctcacgcttg
      421 ccgtcgcggc ggtcggcgca aacggcgcgg gcgtaaagaa accgcctcgc acggagaaca
      481 tagcgccgcc ggtgctgttt gttccggcga ccggctgacc gattgcgctt tcgatttgat
      541 agacgccgcc cgcgcctccg gcagtcgctc cgccgcccga agaaattacc gattgttcga
      601 gcgtgtaagg cggattgccc gccacctgcg cttcggcgac ggcgacaaag gacgcaaagc
      661 cgattgccgc cgtcaacata aatctgatta tcgtcttcat tttattcccc ttttaccggc
      721 aaacgccggt tttcggtttg cctgcgcaga gcctctgttt gaaagggcaa ttatcgcctg
      781 aatcgcaatc gccctttcag acggtttcgc tcgaagttat ttattttgcg gcttcgtcgt
      841 tttctcgtcg ggtttcgcgg tcagggcggg cgcatcgact tcctgaacgg gaaaacgcaa
      901 atccgtgtag cgcgagatgc ggctcggagt gaccgaaccg tccggctgtc gaacgttgcc
      961 caattcctcg tcggcgcggt ttccgacgac gtgccagctg aatttaacgt ttgactttcc
     1021 ctgcgccaat tcgacgacct caaacccgtc ggcggttttg ttggtcacgt aaacgccgtt
     1081 gcagtcgcct tcgagctgga taaaaacgcg caaaggatgt ttatcgttga ccgtgatgcc
     1141 ggcggcgaaa atcgggtcga gcacgatccg cgcgcgacct tcgacgagct gtccgacgcc
     1201 gtaatcttca aacaaaactt cgggcgcttc gggcgcaaac atcacgcgct gtctttgctc
     1261 ggtgtcgcgg accagcgtgg aaaccgttcc cgttccgttg attttgaact gcgtcgtccc
     1321 gttccagtaa ttgacccgtg ttatagagcc gaagttgtca ctgtagatta cctgagaagc
     1381 gccggcacta ttagttctgc cataaatggc ggtggtaaac ccggtcgccg ccagaccgga
     1441 gcctgctact aaagtgttgc cagcctgatt gtttcccgtc gctacgattc cggtgccgtt
     1501 ggtattatta ttctgtcccc agactccggc tgccccgctg ccagcctgat ttgtgacgcc
     1561 gaccaatccc gcgccgcctc cggttcccgc gacggcgcta ttaactccgg taataatatc
     1621 ggagttggcg cccgcgttct ggacggtcac taatcccgta aaagttttgt tgccgccgat
     1681 attcgtttga ttggtggaaa ggtcgacgaa attctgggtc gagctgcccg ttccgccgtt
     1741 cgcgatttga acgacgccgg tcacgtttcc ggctgtcgcg gcggtattcg ccgtgttcgc
     1801 cgtgttggcg ttggcgaccg ttccggttac cttgccgccg tcaatcgaat taatctgcgc
     1861 gttcgtcacg cagccgacgc aggcgctcga aagcgagtct gccgatgacg aatttatcgc
     1921 gcggttggaa aacggagcgg aagtgatttc ctgtcgcggc gtcagaacgg ttgagccgac
     1981 ctgaatttcc agaaacctcg cgctcgttgt gttgaacgcc gccgcgccga agtcgagctt
     2041 tacggtaaag attccgttca cgaccgacac gagcgagctg atctgggtcg tattatccgg
     2101 aatctgattg ccgttggtcg ccgcgtcgaa caaacgaaac gtcatcgtat aattgccgtt
     2161 ggtcggctgc gtcacgctcg aatcggtgaa tcgtccctga taggtgaacg ccgtggtctg
     2221 agcggaaacg cagagtgaaa gaacgaacga aaagactatt aggagcatca agcgtcgtat
     2281 gaaaattaaa agaattttgt gcattggata ttttcctttt cggctggaat cgttttagta
     2341 aaaattgatg cagaagatat ctgctcgaat tttgcaaatc ttttgattgg caaaatatta
     2401 tactcataaa agggacattt gcaataagtt tgcggaagaa gtcggactgc cgtttgagag
     2461 cggtccgaga cgctcatacg gactgcgcgc ggcttcctcc atcttgaggg ttttgtcgaa
     2521 acgttcgcgc taaatgcccg gtggctcggg aaatgcggcg cgcgtctcgc gccgagcccg
     2581 acagggagag ttatttatga tttcctgtag cggcatactg acaatcggct cgcgccgggt
     2641 ttaaactaaa gttcaagccg acttattgaa ggatttttgc aaggaggatt atggacgata
     2701 aacgattaga ggaagctatg aaattaaacg acgaatcgcc ggaaatcagc gcggacgaag
     2761 agaagctgct cgaccagctc ggtctgttcg gagaatcccg tcgtcagttt ctgggacagg
     2821 gcatcggcgg cgggctcggc attttcgcgc ttcagctgtt ggcgaaggaa caggcgctcg
     2881 cagcgctgag cgcaccgccg gacgcggtat tcgcgcagaa cgccgggctg gaaaacgccg
     2941 tcaggatttc attgaaaatc aacggcgcgg cgcagagttt agaggtcgat tcgcggatgt
     3001 cgctgctcga cacgcttcgc gaaagactcg atttgaccgg cacgaaaaaa ggctgcgatc
     3061 agggacagtg cggagcttgt acggtgctcg ttaatggaag ccgcgttttg tcatgcctga
     3121 ctctggcggc gcagtgccag ggaaaagaag tgacgaccat cgagggatta gccaaaggcg
     3181 acgagcttca cccgatgcag gctgccttta tcaggcacga cggttttcag tgcggttatt
     3241 gcacgcccgg gcaaatctgc tcggcggtcg ctttattgaa cgaagcgaaa aacggcgacg
     3301 ccagccacgt cacgaaaaat ctgcgtacaa acgcgcggaa cataaaatta acggacgaag
     3361 aaatcaggga acgcatgagc ggcaacatct gccgctgcgg cgcttatccg ggaattgtcg
     3421 cggcgatcag agaagttcag tcgggacgcg aaacggcgca gacctggcat tttgccaccg
     3481 atgatgaaat cgccgccgtc atgaacgagg aggaaaagag cgatgaaatc gtttaaatat
     3541 tcgagtgcga cagatgcggc gggcgccgtc cgcgcggttt ccgcaaaccg atcggcaaaa
     3601 tttctggcgg gcgggactaa tctgatcgac ctgatgaagg aatacgtcga gcgcccggat
     3661 gagcttgtag acgtctcgca gctgaaatta gcgcagattc gcccgacgtc gaacggggtt
     3721 tcaatcgggg ctttagcaaa aaacgccgat acggcgaatc atccgctcgt ccggcagcag
     3781 tatcctctac tgtccatggc gattttagcc ggtgcttccg ggcagcttcg caatatggcg
     3841 acgaatggcg gtaatttaat gcagcgcacc agatgccagt atttttacga catcgcgatg
     3901 ccgtgcaaca agcgcgagcc gggaacgggc tgcggcgcgc tcgaaggctt gaaccgcatc
     3961 cacgccatct tcggttggag cgagaagtgc gtggcgacat atccgggcga catggcgaac
     4021 gcgctctacg cgctcgacgc cgtggtcagg gtcagggggg ccgaagggcg cgagcgcacg
     4081 attcccgtcg gggagtttca ccgtctgccc ggcgacacgc ctgagcgaga caacaacctc
     4141 cggcacggcg aactcatcgt ggcgattgat ctgccgaagt cgaatttcgc gcagaactct
     4201 tactacctga aagtccgcga ccgcgcgagt tacgccttcg cgctcgtcgc ggtcgccgcc
     4261 gcgctcgaac tcgatggcgg cacgatcaag caggcgcgcg tcgtgctcgg ctcggtggcc
     4321 cacaagccct ggcggagcgc ggaggcggag gccgcgctcg cgggcaggcc cgcctcggaa
     4381 gaatcgttcc gccgggcggc cgacgcggca ctcaaggacg cgcggccgct cgcgcacaac
     4441 gcatacaaag tcgagctggg taaacgcgcg atagttcgct cgctgatgcg tgcgagcaag
     4501 ctggcctgac gggttgggaa ccggggagga aattgggatg ccgaggtata tcgggaaaga
     4561 gatgagccgc gttgacggcg tggcgaaggt aacgggcaag gcgaagtacg cggcggagtt
     4621 ccgcgcgccg aacctcgcct acggcttcat cgtgctgggc acggtggcga agggcacgat
     4681 caagtcaatt gatacgcgcg aggcggaagg cgcacccggc gtcgtgcgcg tcttcacgca
     4741 tctgaacgcg ccgaaactgg ggccgaaggc atcgcacgag caggccccgc cgcgcgcgac
     4801 ggaagagaag gacaagtcgt tccgcgcgct ccagtcggac agaatttact tcaacatgca
     4861 gccggtggcg ctcgtcgtgg ccgaaaccta cgagcaggcg cgctacgccg cccgcctcgt
     4921 caaagtggct tacaacgcgg agcggcacat gaccgacacg gaggcggtgc gcgaacgcgc
     4981 gcgcgtgccg tcgcaggggc cgcccccgaa gccgcgcggc aacccggagg cggccatgaa
     5041 gagcgcgccc gtccgtgtcg aggccgagta ccgcatcccc gtcgagcatc acaatccgat
     5101 ggagccgcac gcggccgtcg ccgtctggga aggcgaccgg ctcacggtct tcgacaagtc
     5161 gcaggaagtc tacaacgtgc gcaagcacct cgcgtcgagc ttcggcgtgc cggagacaaa
     5221 cgtgcaggtc atctcgcctt acgtcggcgg ggctttcggg tcgtctttga ggcccaatta
     5281 ttacccggct ttgacggcga tggcggcccg cgagatcaag cgtcccgtca aggtcgtcta
     5341 cacacggacg cagatgttca ccggccacgg ctaccgcccg cacacgatcc agaagatcgc
     5401 gctcggcgcg gagcgaagcg ggaagctcac ggcgatgatt caccacgccg tgcacaac
//
LOCUS       DASWNE010000415         6409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_683766, whole genome shotgun sequence.
ACCESSION   DASWNE010000415 DASWNE010000000
VERSION     DASWNE010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6409
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683766"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="VGB68_08365"
     CDS             complement(<1..907)
                     /locus_tag="VGB68_08365"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEY0049282.1"
                     /translation="MEEIRKFSRYFLPYKGRIILGIVFILFGMLFGLLVPYLVGQAID
                     DMSAQMTWEKIVYYPLVILGANLVGGIFLFWQRRLLINSSRDIEYDMRRDYYAALVNQ
                     PQEFFQNHRVGDLMARATNDLAAVRQIVGPMILYSFQAIFALLVVLPILLSISVRLTL
                     LLFLTMPLVSLTVKFLGERIHVRFEKIQEFFSGITATAQENLSGVRVVRAYAQEDAEI
                     EKFQVLNREYAEKNLALVKYAAAMRPLLFFFIGLGFVIIVAVGVPMAVRGEITAGDFT
                     AFILYLQRMIWYLIALGYVVNLYQRG"
     gene            complement(1008..1421)
                     /locus_tag="VGB68_08370"
     CDS             complement(1008..1421)
                     /locus_tag="VGB68_08370"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY0049283.1"
                     /translation="MSELEISLLEKAQAVFREVPYIKLLGMELLDLREGEAVLRLKMR
                     DELRQPHGILHGGATASLIDTATAFAVITKLEPGEKASTVDLTVHYLRPVIDGTIICT
                     ARIVKAGRRLLTVSADVVDDKEKLIATAISTYSKV"
     gene            complement(1520..2248)
                     /locus_tag="VGB68_08375"
     CDS             complement(1520..2248)
                     /locus_tag="VGB68_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049284.1"
                     /translation="MLSSNPAKLFYLVLFFSICFSACGFWRQAEENSNASPTPFAAQE
                     IPSEIPFSTREPEVFQGEIIVSGFIDDEKSERKTFIARSGNRRLTVYAAGEKTQVSFL
                     RIDEERAFSVSREKKIYTESAFNRASGASSSENFNDFLTVARLNAKASAAFEKLGAEN
                     GLSKFRVRLNNSETSEILIYVDENLKIPVRQEFYSRSGEGKTLLYTVELKNFQLQTDE
                     KLFEPPKDFRKVSREEFDKTVRQE"
     gene            2292..3038
                     /locus_tag="VGB68_08380"
     CDS             2292..3038
                     /locus_tag="VGB68_08380"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY0049285.1"
                     /translation="MNLWIMQTIYILSALLILPIAPFLYLQGRYVRRKVGLLPDAAGE
                     KTGKFGSCETAVRLLAIGESTVAGLGAKTHETALAGQFARVLSEKIGKSVRWHVIGKN
                     GVTAKRALKELVPQMPAGEKFDYIIVGLGGNDVLKLSSPLKWRRNMIKILRALREKHP
                     ESVIFITNVAAVKLSPVLPEPIKFILSNLSRLHNANIKQFVRQFDRVYYYEQPDTVPP
                     DFFSDGIHPSEAGYKIWSEEIIKTMSRNER"
     gene            3028..4518
                     /locus_tag="VGB68_08385"
     CDS             3028..4518
                     /locus_tag="VGB68_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049286.1"
                     /translation="MNAELAKPELESFLRSRREWLLVFDSTGKNFSLQSSEIEISFER
                     EKLFCGFTSEKGFQTWRIVGCRFENEKIALDLTRNFGKENEKISLVPRISAFDLSREA
                     ELARLEKAQQIANLIIESIPPAKLVRVDLNRETGRFAQIIFEDFRASRIAAVSDVSDS
                     LTPEILVSTAIIWLEKLRNRKKNAVGKVWILAEAKPAKDLSKLHALLRENRKDKIRIF
                     EVSRPGAKTQNKELKEIPALSIADLWRGKSAKIQAVENLESSETARKIVELAPGEIDV
                     VFSKNGETLRFSGLAFARVRKISGEEKTWFGVETRKRILDAKTFGELVELIEELKAYR
                     RFDSANKQHAFYRLAPESWLEAVLRKNIRLLDANLVLSPLYHQFRAERDKIDLLALRK
                     DGRLVIIELKVAPDRAAVFQAADYWRKIELQRRNGNLQKAKIFGDLKISDAPALIYLA
                     APALSFHRDQTFLARTIAPEIEIYRFDLNKNWRANLKVLRREKLTN"
     gene            complement(4654..5367)
                     /locus_tag="VGB68_08390"
     CDS             complement(4654..5367)
                     /locus_tag="VGB68_08390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0049287.1"
                     /translation="MKILIVEDERHLAEGLRFNLEAENFAVETALDGAEALRLLIEEN
                     KQFDAIVLDVMLPEVDGFTVAQTLRKKEIFIPILMLTARNRPEDVLRGFEAGADDYLP
                     KPFELKIFLARLHGLLRRREWFRKAEKSTSSAVNGNFVINGKTIDFESLELQSGAETI
                     HLTLMEVKLLRYLIENAGKPVSRKTILEEVWDLREDTDTRAIDNFIVRLRRYLEDEPE
                     KPKILQTVRGIGYKFVKEN"
     gene            complement(5420..6334)
                     /locus_tag="VGB68_08395"
     CDS             complement(5420..6334)
                     /locus_tag="VGB68_08395"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0049288.1"
                     /translation="MRVARRRKSSIFFLVLGICLVVLAVALYVGWILLNLQQVALLVL
                     GIVFFSLIITGLILNTLFLIREIRRNEQQDAFLNAVTHELKTPIASIRHYLETLKTRD
                     IKPAKQQEFYDVMLADSERLLNTVEQILLASRAREQKRLLNISEINLLTLLRECAERV
                     RHRYDLPENAISLPSAEDKIKISGDTDELQAVFMNLLDNAVKYSQEDIKIALNVKNLN
                     EKFVIVRIKDSGIGLPASEMKRIFRRFYRVPNRATQKAKGTGLGLFIVRSIVDKHGGK
                     IWAESAGPGAGSTFMVKLPKIVNDKSKL"
BASE COUNT         1657 a         1651 c         1575 g         1526 t
ORIGIN      
        1 tgccgcgctg gtaaagattg acgacgtagc cgagcgcaat cagataccaa atcattcgct
       61 gcagatacag gataaacgcc gtaaaatcgc ccgccgtaat ctcgccgcgc accgccatcg
      121 gaacgccgac ggcgacgata atcacaaaac ccagaccgat aaagaaaaac agcagtgggc
      181 gcatcgccgc cgcgtatttg acgagcgcga gatttttttc cgcgtattcg cggttcagaa
      241 cctgaaattt ttcgatttcc gcgtcttcct gcgcgtaggc gcggacgact cgcacgcccg
      301 aaagattttc ctgcgcggtc gccgtgatgc cggagaaaaa ttcctgaatt ttttcgaacc
      361 ggacgtgaat ccgctcgcct aaaaatttaa ccgtcaaact gacgagcggc atcgtcagaa
      421 aaagcagaag cgtcagccgg acgctgatgc tcaagagaat cggaagcacg accagcagcg
      481 cgaaaatcgc ctgaaacgaa tataaaatca tcggtccgac gatttgccga accgccgcca
      541 aatcgttggt cgcccgcgcc atcaggtcgc cgacgcggtg attctgaaaa aactcctgcg
      601 gctgattgac cagcgcggcg taataatcgc ggcgcatatc gtattcgatg tcgcgcgagc
      661 tatttatcaa aagcctgcgc tgccagaaaa gaaagatgcc gcccacgagg ttcgcgccca
      721 ggatgaccag cggataatag acgattttct cccacgtcat ctgcgccgac atatcgtcaa
      781 tcgcctgccc gaccagataa ggcaccagca atccgaacag catcccgaac aggataaaaa
      841 caatgccgag aataatgcgc cctttatacg gcagaaaata acgcgaaaat tttctaatct
      901 cttccataaa tatgagttga ccgagttgac tgaaagttga ccgaagtttt ttaactcagt
      961 caactcggtc aactttcagt caactcagtc aactttcagt caactcacta aactttcgag
     1021 taagtcgaaa tcgcggtcgc aatcagcttt tccttatcgt cgacgacgtc tgccgaaacg
     1081 gtcaaaagcc gtcgtccggc tttgacgatg cgcgccgtgc agataatcgt cccgtcaatc
     1141 accggacgca ggtaatgaac cgtcaaatcg accgtcgaag ccttttcgcc cggctccaat
     1201 ttggtaatga cggcaaacgc cgtcgccgtg tcgatcaggg acgcggtcgc gccgccgtgc
     1261 agaatcccgt gcggctggcg cagctcgtcg cgcattttca gccgcaaaac cgcctcgccc
     1321 tcgcgcaaat cgagcaattc cataccgagc agtttaatat acggaacttc gcggaagacc
     1381 gcctgcgctt tttccagtaa cgatatttcg agctcactca taaacgtgtc cgaaaccggc
     1441 tgccggcggc gttcgggatt ccggatttta gattttagat taaagattgt taacggcaaa
     1501 tctaaaatct aaaatctaat tactcctgcc ggacggtttt atcaaattct tcccttgaaa
     1561 cctttcgaaa atctttcggc ggctcgaaaa gcttttcgtc cgtctgcagc tgaaagtttt
     1621 ttaattcgac cgtgtagagc aaagttttcc cctcgcccga gcggctgtaa aattcctgcc
     1681 tgaccggaat ttttaaattt tcgtcgacgt aaatcagaat ctcgctcgtt tccgaattgt
     1741 ttaagcgcac ccgaaatttg ctcaaaccgt tttccgcgcc gagcttttca aaagcggcgg
     1801 aagcttttgc attgagccgg gcgacggtca gaaaatcatt gaaattttcg ctgctcgatg
     1861 ctcccgaggc tcgattaaag gcgctttccg tataaatttt cttttcccgg gaaaccgaaa
     1921 aagcgcgctc ttcgtcaatc cgtaaaaacg agacctgcgt tttttctccg gcggcgtaaa
     1981 ccgtcagacg ccggttgccg ctgcgcgcga taaaagtttt gcgctcggat ttttcgtcgt
     2041 cgataaaacc ggaaacgatg atttcgccct gaaaaacttc cggctctctg gttgaaaacg
     2101 gaatttcgct cggaatttcc tgcgccgcga aaggcgtcgg gctggcgttt gaattctcct
     2161 cagcctggcg ccagaatccg cacgccgaaa agcaaatcga aaaaaagaga acgaggtaaa
     2221 acagttttgc cggatttgac gaaagcatct gtaaaagtaa aattttatca tacggattgg
     2281 agaagccgac aatgaacctc tggataatgc agacaattta tattttgagc gcgcttctga
     2341 ttctgccaat cgcgccgttt ttgtatttgc aggggcgata cgtgcgccgc aaagtcggct
     2401 tgctgccgga cgcggcgggc gagaaaacgg gaaaattcgg cagctgtgag acggctgtca
     2461 ggctcctggc aatcggcgaa tcgaccgtcg ccgggctcgg cgcaaaaacg cacgaaacgg
     2521 cgctcgccgg acaattcgcc agagttttga gcgaaaaaat cggcaaatcc gtgcgctggc
     2581 acgtcatcgg caaaaacggc gtcacggcaa aacgagcttt aaaagagctt gtcccgcaga
     2641 tgccggcggg cgaaaaattc gattacatca tcgtcggctt gggcggcaac gacgttctga
     2701 aactcagcag tcctttaaaa tggcggcgca atatgataaa aattcttcgc gctctgcgcg
     2761 aaaaacatcc cgaatccgtg atttttatca ccaacgtcgc ggcggtcaag ctctcgcccg
     2821 ttttgcccga accgataaaa tttattttgt ccaacctttc gcgcctgcac aacgccaaca
     2881 tcaagcagtt tgtccgtcaa ttcgaccgcg tttactatta cgagcagccc gacacggttc
     2941 cgcccgattt tttcagcgac ggcattcacc cgtccgaagc gggctacaaa atatggtcgg
     3001 aagaaattat taaaacgatg agccgcaatg aacgctgaac ttgccaaacc ggagctggaa
     3061 agttttctgc gctcgcgccg cgaatggctt ttggttttcg actcgacggg aaaaaacttt
     3121 tctctgcaat cgagcgaaat cgaaatctcg ttcgagcgcg aaaaattatt ttgcggtttt
     3181 acgagcgaaa aaggctttca aacgtggcga atcgtcggct gccgttttga aaacgaaaaa
     3241 atcgcgctcg atttaacgcg caatttcgga aaagaaaatg aaaaaatctc gcttgtgccg
     3301 cgcatttcgg cgtttgattt gagtcgcgag gcggaactgg cgcgcctcga aaaagcgcag
     3361 caaatcgcca atctgattat cgagtccatt ccgccggcaa agctcgtccg cgtcgattta
     3421 aatcgggaaa ccggacgctt cgctcaaatc atctttgaag attttcgggc ttcccgaatt
     3481 gccgccgtca gtgacgtttc cgattcgctg acgccggaaa ttctggtttc gacggcgatt
     3541 atatggctcg aaaaattgcg gaatcgaaag aaaaacgcgg tcggaaaagt ttggatttta
     3601 gccgaagcga agccggcgaa agatttgtcg aagcttcacg ctttgctgcg cgaaaaccgg
     3661 aaagacaaga taaggatttt tgaagtttcc cgcccgggcg caaagacgca aaacaaagaa
     3721 ttaaaggaaa ttccggcttt atcaatcgcc gatttatggc gcggaaaatc cgcgaaaatt
     3781 caggcggtcg aaaatcttga atcgagcgaa acggcgcgaa aaatcgtcga gcttgcgccc
     3841 ggcgaaattg acgttgtttt cagtaaaaac ggcgaaactc tgcggttttc gggactcgct
     3901 ttcgcgcgcg tcagaaaaat ttccggcgaa gaaaaaacct ggttcggcgt ggaaacgcgg
     3961 aagcggattc tcgatgcgaa aaccttcggc gagcttgtag aattgattga agagcttaaa
     4021 gcttatcgcc gttttgattc tgcgaataaa cagcacgctt tttaccggct cgcgccggaa
     4081 agctggctcg aagcggtttt gagaaaaaac atcaggctgc tggacgcgaa tcttgtttta
     4141 tcgccgcttt atcatcagtt tcgcgccgag cgcgataaaa tcgatttgct tgctttgaga
     4201 aaagacggtc ggctcgtgat tatcgagtta aaagtcgcgc ccgaccgcgc cgctgttttt
     4261 caagccgccg attactggcg aaaaatcgag ctgcaaagaa gaaacgggaa tctgcaaaag
     4321 gcaaaaatct tcggcgattt gaaaatctcg gacgcgcccg cgctaattta tctcgctgcg
     4381 ccggcgctct cgtttcaccg agaccagacg tttctggcgc ggacaatcgc gccggaaatc
     4441 gaaatttacc gctttgattt aaacaaaaac tggcgcgcga atttgaaagt tttgcgaaga
     4501 gagaaattaa cgaattaacc acggatttac acggataacc gcggattttt aacaggattt
     4561 acaggataat ttaaaacttg caagcgtcga gctttctttt tatcttgtaa atcctgttag
     4621 aatctggtgt ttataccgtg caaatctgtg gtttcaattc tctttcacga atttgtagcc
     4681 gatgccgcgg acggtttgca ggattttcgg tttttccggc tcgtcttcga ggtagcggcg
     4741 caggcggacg ataaaattat caatcgcgcg cgtgtccgta tcttcgcgca aatcccagac
     4801 ttcttccaga atcgtcttgc gcgagacggg cttgcccgcg ttttcaatca ggtagcgcaa
     4861 aagcttgact tccatcaacg tcaggtgaat cgtttccgcg ccgctttgca attccaggct
     4921 ttcaaaatca atcgtcttgc cgttgataac aaaattaccg ttgacggcgg aagaagtcga
     4981 tttttccgct ttgcgaaacc attcgcgccg ccggagcaga ccgtgcaggc gcgccagaaa
     5041 aatcttcagc tcaaacggtt tcggcagata atcgtccgcg cccgcttcaa atccgcgcag
     5101 cacgtcttcg gggcggttgc gcgccgtcag cattaaaatc ggtataaaaa tttctttttt
     5161 tctgagcgtc tgcgcgaccg taaaaccgtc cacttccggc agcatcacgt ccagcacaat
     5221 ggcgtcaaat tgtttatttt cttcaatcag caggcgcagc gcctcggcgc cgtccaatgc
     5281 cgtttcgacc gcgaaatttt cggcttcgag attaaagcgc aaaccttccg ccagatgccg
     5341 ctcgtcttcg acgattaaaa ttttcatagt ctatgaacta aaaacctaca gaaactctat
     5401 cgaaacgcct cgcagttctt cacaatttcg atttatcgtt aacgatttta ggcagtttga
     5461 ccataaacgt gctgccggcg ccgggtccgg cgctttccgc ccagattttt ccgccgtgtt
     5521 tatcgacaat cgagcgcacg ataaaaagtc ctaaacccgt tccttttgct ttctgcgtgg
     5581 cgcggtttgg aacgcggtaa aagcgccgga aaattcgttt catctctgat gcgggcagcc
     5641 cgatgcccga atctttgatg cgcacgatga cgaatttttc gttcagattt tttacgttga
     5701 gcgcaatttt tatgtcctct tgcgaatatt ttacggcatt gtccagcagg ttcataaaaa
     5761 ccgcctgaag ctcgtccgtg tcgccggaaa tttttatctt gtcttcggcg gacggaagcg
     5821 aaatcgcatt ttcgggcaaa tcgtaacgat gccggacgcg ctcggcgcat tcgcgcagaa
     5881 gcgtcagcag atttatctcg gaaatattca aaagacgttt ttgctcgcgg gcgcggctcg
     5941 ccagcagaat ctgctcgacc gtgttcaaaa gccgctcgct gtccgccagc atcacgtcgt
     6001 aaaattcctg ttgtttggcg ggcttaatgt cgcgcgtttt cagcgtttcc aggtaatgcc
     6061 ggatggaagc aatcggcgtt ttcagctcgt gcgtgacggc gttcagaaaa gcatcctgct
     6121 gttcgttgcg gcgaatctcg cggatgagaa ataacgtgtt taaaatcaag cccgtaataa
     6181 ttaacgaaaa aaagacgatg ccgagcacca gcagcgccac ctgctgcaaa ttcagcaaaa
     6241 tccagccgac atagagcgcg accgcgagga caaccagaca gatgcctaag actaaaaaga
     6301 aaatcgagct tttgcggcga cgagcaacac gcattttttg attttaaagg taaaagcaaa
     6361 aaggcgaaag gcggaaggca aaatggaaat cgatgatgtt tccattttg
//
LOCUS       DASWNE010000416         1527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_544317, whole genome shotgun sequence.
ACCESSION   DASWNE010000416 DASWNE010000000
VERSION     DASWNE010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544317"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1207)
                     /locus_tag="VGB68_08400"
     CDS             complement(<1..1207)
                     /locus_tag="VGB68_08400"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEY0049289.1"
                     /translation="MTKINETNVNLFDFSRKLEDAFSSVANLFKDSDGDRLPDILSGN
                     PDDYKTRLLFDREGYMQASLNNKENLGVGGTQRVTADSTPEELLRDPRVRAMLDAIAY
                     AEGTRNNGDYGRVVNGTVLGPSNANAPYDRSLVGRRNVVVNDLTRHPNLAVRWANGQP
                     PSSAAGRYQFLYRTWQGLNMPDFSPRSQDLGAIKLMQRRGMIEPLLRGDFAEAIHRGA
                     PEWASLPVPGGGSYYGGQGAKTLSSLREVYGNALQRYQGTEPPTTTPTNPNPTPSNPA
                     TGGSLRRGQNGRAVEALQDRLIRLGLMTEAQQRTGPGIFGPQTEAAVKKFQQSVGLSQ
                     SGRFDNATRGAMNKIFSGDVERGARGDLVRQMQEKLVRLGYMTRRQVNTGPGIFGPQT
                     DAALRRFQSE"
BASE COUNT          322 a          433 c          407 g          365 t
ORIGIN      
        1 gttcggattg aaaacgcctg agcgcggcat ccgtttgcgg tccgaaaatt cccggtccgg
       61 tgttgacctg ccggcgcgtc atatagccga gccgcacgag tttttcctgc atctggcgga
      121 caaggtcgcc gcgcgcgccg cgttcgacgt cgccggaaaa gattttattc atcgcgccgc
      181 gcgtggcgtt gtcgaaacgc ccgctctggc tcaagccgac gctttgctga aatttcttga
      241 cggcggcttc ggtttgcggt ccgaaaattc ccggtccggt tctctgctgc gcctcggtca
      301 tcaaaccgag acgaatcaat ctatcctgta aagcctcgac cgcgcgaccg ttttgaccgc
      361 ggcgcaagct gccgcccgtc gccggattcg acggcgtcgg attcgggttg gtcggcgtcg
      421 tggtcggcgg ctcggttccc tggtaacgct gtaaggcgtt gccgtaaact tcgcgcaggc
      481 tgctcagagt tttcgcgccc tgcccgccgt aatagcttcc gccgccggga accggcagac
      541 tcgcccactc gggcgcgccg cgatgaatgg cttcggcaaa atcgccgcgc agaagcggct
      601 cgatcattcc gcgccgctgc attaatttaa tcgctcccaa atcctgcgag cgcggcgaaa
      661 aatccggcat attcaagccc tgccacgtgc gataaagaaa ctggtatctg cccgccgcgc
      721 tgctcggcgg ctgaccgttt gcccagcgca ccgccagatt cgggtgacgg gtcaaatcgt
      781 taacgacgac gttgcgtctt ccgaccagcg agcggtcata cggcgcgttc gcattgctcg
      841 gaccgagaac cgttccattc acgacgcgac cgtagtcgcc gttatttctc gttccttcgg
      901 catacgcgat agcgtcgagc atcgcgcgga cgcgcgggtc gcgcagaagc tcttccggcg
      961 tcgaatcggc ggtcacccgc tgcgttccgc cgacgccgag attttccttg ttgttcagcg
     1021 aagcttgcat ataaccttcg cggtcaaaca aaagtctggt tttgtaatcg tccggattgc
     1081 cggagagaat atcgggaagc cggtcgccgt ccgaatcttt aaacagattg gcgacactgc
     1141 tgaaagcgtc ttccaatttg cggctgaaat cgaataaatt gacgttcgtc tcgttaatct
     1201 tggtcatgga aatttctccg gcagtgcaga ttttaaaacc tttagctaag ttatcggaga
     1261 aattgcggaa atgtttcctg atttttaaag cgtgacgctt taaaaaagag gtaagcggta
     1321 aacggtaagt ggtaagtcgc aatcattgaa tatcgaattt ttatttaaaa ctcgatattc
     1381 agaataacga acttaccgct taccgtttac cgcttaccac ttttttaaag cgtcccgctt
     1441 taaaaatcag gaaacattcc cgcaatttct ccgataactt agctaaaggt tttaaaatct
     1501 gcactgccgg agaaatttcc atgacaa
//
LOCUS       DASWNE010000417         1744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_650048, whole genome shotgun sequence.
ACCESSION   DASWNE010000417 DASWNE010000000
VERSION     DASWNE010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650048"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /gene="trmFO"
                     /locus_tag="VGB68_08405"
     CDS             complement(<1..1145)
                     /gene="trmFO"
                     /locus_tag="VGB68_08405"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEY0049290.1"
                     /translation="MMEVNIIGGGLAGVEAAWQAAESGGAKVKLYEMRPVQQTPAHRT
                     DKLAEIVCSNSLKTDEAGTAPYLLKEELRRGKSLVMEAARLTKIPAGAALAVDRHKFA
                     EYITEKIENHPRIEIIREEVREIPPDEITVIATGPLTSEALTNEIMKLTGDDQLYFYD
                     AIAPIVAADSIDMSIAFKAARYGKGGDDYINCPFTPEQYAHFYNELINAKSVPLKRFE
                     ETHWFESCLPIEETARRGVDTLRFACMKPKGLPDPKTGREPYAAVQLRQENLMADAYS
                     LVGFQNHLRYGEQARVLRLIPGLENAEFLQFGQIHRNTFINSPKILNASLQTRKSPKL
                     FFAGQITGVEGYVESVGTGWLAGLNAARLARNENLISAPAASAVGAL"
     gene            complement(1150..>1744)
                     /locus_tag="VGB68_08410"
     CDS             complement(1150..>1744)
                     /locus_tag="VGB68_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049291.1"
                     /translation="TGEFSSDEKAAGAAIDFLLNRYSEKHRYYHNLNHVNALLVWAED
                     LKSNISDYNCVGLAIWYHDAIYDPQKNDNEIESAKLAARHLSGFGLSKNRIQKVEKMI
                     LATQKHDAATLDTDGKLFLDLDLSILGAGAGIYKDYSRAIRLEYSFVPEDLYRESRKK
                     ILENFLRREFIYFSGELRARLEAAARLNIENEIKELS"
BASE COUNT          397 a          500 c          408 g          439 t
ORIGIN      
        1 caaagcgcgc cgaccgcgct cgccgccggc gcggaaatta aattttcatt tcgcgccagc
       61 cgagccgcgt tcagaccggc gagccagccc gtgccgaccg attcgacgta gccttcgacg
      121 cccgtgattt gaccggcaaa aaacagtttt gggcttttcc gcgtttgcaa gctcgcgttt
      181 aaaatcttcg gcgaattgat aaaagtattc cggtgaatct gcccgaactg taaaaactct
      241 gcgttttcca atccgggaat caggcgcaga acgcgcgcct gctcgccgta acgcaggtga
      301 ttctgaaaac cgaccagcga gtatgcgtca gccatcagat tttcctgtct gagctgcacc
      361 gccgcgtaag gctcgcgccc ggttttcggg tcgggcaagc ctttcggctt catacacgcg
      421 aaacgcaagg tatcgacgcc gcgccgcgcc gtctcttcga tcggcagaca cgactcgaac
      481 cagtgcgttt cctcaaatct tttcagcgga acagatttcg cgtttatcaa ttcgttataa
      541 aaatgcgcgt attgctcggg cgtaaaaggg caattgatgt aatcgtcgcc gcctttgccg
      601 taacgcgccg ctttgaaagc gattgacata tcgatcgagt ccgccgcgac gatcggcgcg
      661 atcgcgtcgt aaaaataaag ctggtcgtcg cccgtcagct tcattatttc attcgtcagc
      721 gcctcggaag taagcggtcc ggtcgcgata accgtaattt cgtccggcgg aatctccctg
      781 acttcttcgc gaataatctc gatgcgcgga tgattttcga ttttttcggt gatgtattcg
      841 gcaaatttgt ggcgatcgac cgccagcgcc gcgcccgccg gaattttggt cagacgagcg
      901 gcttccatca ccagagattt tccgcgtctt aattcctctt tcaacagata cggcgcagtt
      961 cccgcttcat ccgttttaag cgaattcgag cagacgattt cggcgagctt atcggtgcga
     1021 tgcgccggcg tctgctgaac ggggcgcatt tcataaagct ttactttcgc gccgccgctc
     1081 tcggctgcct gccacgccgc ttcgacgccc gccagaccgc cgccgataat attaacttcc
     1141 atcatttttt tatgataact cttttatttc gttttcgata ttaaggcgcg ccgccgcttc
     1201 aagccgggcg cgcagctcgc cgctaaaata aataaattcg cgccgcaaaa aattttccag
     1261 aatctttttc cggctctcgc ggtataaatc ctccggcacg aaagaatatt caagccggat
     1321 ggcgcgcgaa taatctttat aaatgcccgc gcccgcgccg agaatggata aatccaaatc
     1381 caaaaataat ttcccgtccg tatcgagcgt cgccgcatcg tgtttctgcg tcgccagaat
     1441 catcttttcg actttttgaa ttctattttt cgataaaccg aagccggaaa gatgccgcgc
     1501 ggcgagcttc gcgctctcaa tctcattatc attcttctgc gggtcataaa tcgcatcgtg
     1561 ataccaaatc gccagcccga cgcaattgta atccgaaata ttagacttta aatcttccgc
     1621 ccaaaccaaa agagcattaa cgtgattcag attatgataa tagcgatgtt tttccgaata
     1681 tctatttaat aaaaaatcaa tcgccgcgcc cgccgccttc tcatccgaag aaaattcgcc
     1741 ggtc
//
LOCUS       DASWNE010000418         4391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_507132, whole genome shotgun sequence.
ACCESSION   DASWNE010000418 DASWNE010000000
VERSION     DASWNE010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507132"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /locus_tag="VGB68_08415"
     CDS             <1..951
                     /locus_tag="VGB68_08415"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HEY0049292.1"
                     /translation="KAKPDEVKFIMVDPKRLELGLYADIPHLATPIITDPKRAAISLK
                     WAVSQMEKRYKDLAGWGVRNIDGFNVEVKKRNAQEHFDDNGEPWKTLPYIVIIIDELA
                     DLMMVAGKEVEESITRLAQMARAVGIHLVLATQRPSVDVITGLIKANFPARVSFRVSS
                     KVDSRTIIDSNGAERLLGRGDMLFLPPATSQIVRVHGAFVDEKEIQRIVDHVKAQGRP
                     EYDTTITKTEEEMDDGGDLPGKRDPLFADALRTVVSAKRASTSLLQRHLRIGYGRAAA
                     ILDAMVREGYIGDMDGSTRARAILQKAYEDLQEMEEGREL"
     gene            1221..1535
                     /locus_tag="VGB68_08420"
     CDS             1221..1535
                     /locus_tag="VGB68_08420"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEY0049293.1"
                     /translation="MLPNKRHLVPAVFCLLLFNGCRQYIKNSPETFKNNPEISENAVN
                     LNTATAEELEKLPRIGKETAQKIIEHRENHGRFRRAEHLILVKGMSDKKFREIRNFVK
                     TE"
     gene            1719..>4391
                     /locus_tag="VGB68_08425"
     CDS             1719..>4391
                     /locus_tag="VGB68_08425"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEY0049294.1"
                     /translation="MTEKPLKTSAANFSLYPLAWLSVCFALGILLENLLSWQIYLAVC
                     TASAISVLIFIKQKSGVIFLMLSFIAAGALCFQAEKYSVAPNRLKNLYDRQIFVSGEP
                     VEINGILQGKPEPAVGGFFLELKAESVVYKGVEKAVSGSVRLFAPTVDKQIAEEYEQL
                     RLKYGTRLRIACELRREDKFRNAGVVSQKEILDRKQIDATAIVKSPLLIENLGEASSF
                     QPLASVYEQRQDLIVEFKKHFSAQTSGVLIASLLGNRYHLDKATSERFREGGTFHVLV
                     ISGLQITFIGTLIVWIIQIFTRNRLWQFLLASALLWSYSLAVGAEAPVVRAAEMFTIL
                     LFANVIFRQGSLLNALGMSALILLIGRPSDLFDQSFQLTFTCVAAIVATAFPLLEKFR
                     GIGEWHPSTETPVPPNCPKNLKSFCEALYWSEENWRREQQRAVWSCRLFKTPFAEKPE
                     RRKLQTILRYVFETLLVSLVVQAWLIPFLVIYFHRISLIGIFLNVWVGLLMAIESIVA
                     ILAIFAAQISATLAAPLILLTEISNWIMIHAADSLVENGWASFRLPHYSGAMQVVYIL
                     YFVPLAILTRLLHDWKPFSLELKARSSKLAARTAFAAFLVFLLLIIFHPFGAPQPDGR
                     LHFDFLDVGQGDAALITMPDGETLLVDGGGKVNFSSLYVQREGDEPELFEPDAQSIGE
                     AVVSEFLWEKGYSRIDYILATHADADHIQGLTDIAKNFRVRTAIFGRTPPDDAEFIEL
                     YNILQKRNVPLLVAARGDVLNFGDVKIEVLYPEKDESPEAVSDNNRSLVLRVVYGDRK
                     FLLTGDIEKETESFLTGAPEFLQADVIKVAHHGSKTSSTENFVNAVKSKVAVISVGRD
                     SPFGHPKPEVVERWKNAGAQVLTTGENGTISFSTD"
BASE COUNT         1065 a         1063 c         1113 g         1150 t
ORIGIN      
        1 aaagcgaaac ccgacgaagt aaagttcata atggtcgacc cgaaacgctt ggaactcggg
       61 ctttacgccg acattccgca tctcgccacg ccgattatca ccgacccgaa acgcgccgcg
      121 atttcgctga aatgggcggt ttcccagatg gaaaaacgct ataaggattt ggcgggctgg
      181 ggcgtgcgaa acatcgacgg ctttaacgtg gaagtgaaaa aacgcaacgc gcaggagcat
      241 ttcgacgaca acggcgagcc ctggaaaacc ctgccttata tcgtgattat cattgacgag
      301 ctggcggatt tgatgatggt cgccggaaag gaagtcgagg aatcaatcac gcgtctggcg
      361 cagatggcgc gcgccgtcgg catccatctg gttctcgcca cccaaagacc gtcggtcgac
      421 gtcatcacgg gtttgataaa agcgaatttt ccggcgcgcg tttccttccg cgtttcgtca
      481 aaagtcgatt cgcgcacgat tatcgattcc aacggcgcgg aacgcctgtt aggtcgcggc
      541 gatatgcttt ttctgccgcc cgccacctcg caaatcgtcc gcgttcacgg cgcgtttgtc
      601 gacgaaaagg aaatccagcg catcgtcgac cacgtcaaag cccagggcag acccgaatac
      661 gacacgacga ttacgaaaac cgaggaagaa atggacgacg gcggcgattt gccgggcaag
      721 cgcgacccgc ttttcgccga cgctctgcgc acggtcgtca gcgccaaacg cgcatcgaca
      781 tcgcttttac agcgtcattt gcgcatcggc tacggtcgcg ccgccgccat tctcgacgcg
      841 atggtgcgcg aaggctacat cggcgatatg gacggcagca cgcgcgcgcg ggcgattctg
      901 caaaaagctt acgaagattt gcaggaaatg gaagaaggca gggaattgta agttataact
      961 ggtaactgag aaatatcagt cagctgtcag ctttcagctt tcagccttca gcttttaact
     1021 ctgaatttcc acttaacagt taacggttaa cagttatcgg ttgaaaatca gttttccaca
     1081 ggttttgtgg aaaaaccttg tggaaaagtc cttttccaca cacttaattg ttgattttca
     1141 aacgactttt agcattttgc acataacgta tgcaatcaaa catacgaaaa attaagtctc
     1201 gttatttcaa catttccgag atgcttccta acaaacggca tttagttccc gccgtgtttt
     1261 gtttgctttt gttcaacggc tgccgtcaat atataaaaaa ttcgccggaa acctttaaaa
     1321 ataatcccga aatttccgaa aatgcggtta atctaaatac cgcgaccgcc gaagagctcg
     1381 aaaagctccc gcgtatcggc aaagaaacgg cgcaaaaaat tatcgagcac agggaaaatc
     1441 acggaagatt tcgccgcgcc gagcatttga ttttagtcaa aggaatgagc gataaaaagt
     1501 ttcgcgagat aagaaatttt gtaaaaaccg agtagtttca ggataattta acgccaattc
     1561 cggcaagcgt cgcggaatcg atgcgcgaat tttaaattgt aaatttcaaa taaatcggca
     1621 taatgttttt cagatagaat ccggaattgg atttctttcc tttttttcct gaactaatgt
     1681 tattttagag cgaaataatt tcttaaaggc gcttgcccat gactgaaaag cctttaaaaa
     1741 cttctgcggc gaatttttcg ctttatcctc tggcttggct ttcggtttgt tttgcgctcg
     1801 gaatcctgct cgaaaatctg ttgtcgtggc agatttatct cgccgtttgt acagcgtccg
     1861 cgatttccgt tttaattttc atcaaacaaa aatccggcgt tatattttta atgctttctt
     1921 ttatcgccgc cggcgctctg tgttttcagg cggaaaaata ttcggtcgca cctaatcgct
     1981 tgaaaaatct ttatgaccgg caaattttcg tttccggcga gccggtcgag ataaacggaa
     2041 ttttgcaggg caagcccgag ccggcggtcg gcggattttt tctggaattg aaagcggaaa
     2101 gcgtcgttta taaaggcgtc gaaaaagcgg tttcgggaag cgtgaggctt tttgcgccga
     2161 ccgtcgataa acaaatcgcc gaagaatatg aacaattacg gctcaaatac ggcacgcggc
     2221 tgcgcatcgc ctgtgagctt aggcgcgagg ataaatttcg aaacgcgggc gtcgtatcgc
     2281 aaaaagaaat tcttgaccga aagcaaatcg acgcaaccgc gattgtcaaa agcccgcttt
     2341 taattgaaaa cctgggcgaa gcttcgtctt tccagccgct cgcttcggtt tacgagcagc
     2401 gacaagattt aatcgtcgaa ttcaaaaaac atttttccgc gcagacttcg ggcgtgctta
     2461 tcgcatcgct tctgggcaat cgttatcatc tggataaagc tacatcggag cgttttcgcg
     2521 aaggcggaac ttttcacgtg ctcgtcatca gcggtttgca gattactttt atcgggacgc
     2581 tgattgtctg gattattcaa atttttacgc gcaatcgttt atggcaattt ctgctggcaa
     2641 gcgcgctttt gtggagttat tcgctggcgg tcggagcgga agcgccggtc gtccgcgccg
     2701 ccgaaatgtt tacgattctg cttttcgcca acgtaatttt ccggcagggc agcctgctca
     2761 acgcgctcgg aatgtcggct ctgattcttt taatcgggcg accgtccgac ctttttgacc
     2821 aatcgtttca gctaactttc acctgcgtcg cggcaattgt cgccactgct tttccgcttc
     2881 tggaaaaatt tcgcggcatc ggcgagtggc atccttcgac ggaaacgccg gttccgccga
     2941 actgtccgaa aaacttaaaa tcattttgcg aagctttata ttggagcgag gagaattggc
     3001 ggcgcgagca gcagcgcgcg gtctggagct gtcgtctttt caaaacgccg tttgccgaaa
     3061 aaccggagcg ccgaaagctg cagacgattt tgcgctatgt gtttgaaacg cttctggttt
     3121 cgctcgtcgt gcaggcgtgg ctcataccgt ttctggtgat ttattttcat cggatttctt
     3181 taatcggaat ttttctcaat gtctgggtcg gtttgctgat ggcgattgaa agcatcgtcg
     3241 ccattcttgc gatatttgcc gctcaaatca gcgcgacttt agccgcgccg ctgattctat
     3301 taacggaaat ttcgaattgg attatgattc acgcggctga ttcgctcgtc gaaaacggct
     3361 gggcttcttt ccgtctgccg cattattccg gcgcgatgca agtcgtttat attttatatt
     3421 ttgtgccgct cgccattttg actcgtctgc tgcacgattg gaaacctttt tctttagagc
     3481 ttaaagcgcg gagctcaaag ctcgcggctc gaacggcttt cgccgcgttt ttagtttttc
     3541 ttttattgat aatttttcat cccttcggcg ctccgcaacc ggacggcagg ctgcacttcg
     3601 attttctcga tgtcgggcag ggcgatgcgg ctttaattac aatgccggac ggcgaaactt
     3661 tattggttga cggcggcggc aaagtaaatt tcagctcgct ctacgttcag cgcgaaggcg
     3721 atgagccgga gcttttcgag cctgacgcgc agagcatcgg cgaagcggtc gtgtcggaat
     3781 tcctgtggga aaaaggctat tcgcggattg attacatcct ggcgacacac gccgacgccg
     3841 accatattca gggtctgacg gatatagcaa aaaactttcg cgtgcgcacg gcgattttcg
     3901 gcagaacgcc gccggatgac gcggaattta ttgagcttta taacattttg caaaaaagaa
     3961 acgttccgct tttggtcgct gcgcgcggcg acgttttaaa tttcggcgat gtcaaaatcg
     4021 aagttcttta tcccgaaaaa gacgaatcgc ccgaagcggt ttccgataac aatcgctcgc
     4081 tggttttgcg cgtcgtttac ggcgacagaa agtttctgct gacgggcgat atcgaaaagg
     4141 aaaccgaaag ctttttaacc ggcgcgccgg aatttttgca ggctgatgtc ataaaggtcg
     4201 cgcatcacgg aagcaaaact tcttcgactg aaaattttgt aaatgccgta aaatcgaaag
     4261 ttgcggttat cagcgtcggt cgtgattcgc cgttcgggca tccgaaaccg gaggtggtcg
     4321 aaagatggaa aaacgccggc gcgcaagttt tgacgacggg cgaaaacggc acgatttcct
     4381 tttcgacgga c
//
LOCUS       DASWNE010000419         1606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_14410, whole genome shotgun sequence.
ACCESSION   DASWNE010000419 DASWNE010000000
VERSION     DASWNE010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14410"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..>1606
                     /locus_tag="VGB68_08430"
     CDS             33..>1606
                     /locus_tag="VGB68_08430"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEY0049295.1"
                     /translation="MKCRKLCPAAFFVLVFVLPQFAQTKNFNRARTFDVQHYTIRTAF
                     ERSTKTVFGDTSIRLKPLKANLTTVELDAAGFKLDFVRLEDSGVNLQYRQAGEKLIIT
                     LDKAYAPKDSISIRIGYSCQPQQGVYFVDALTEKNKIIRDAQIWTQGEPEEAHHWFPS
                     FDFPDDKATTEQFLTVEAGETAIANGELLETLENSNGTKTFHYKISVPHSVYLTSFVV
                     GKYSKVADSYKNIPLGFYVYPGREATARKAFGKTKNMMRIFEDYTGVAFPYSKYDQTV
                     VANFQFGGMENITATTHADTEIFAVDYMPDEAEDLVSHELAHSWFGNLVTCRNWAELW
                     LNEGFATFMEAAYREKAYGRADYMRKIKDDATRFIIDEAVNKNRHGLFNRLARPDNSI
                     FDTTNYQKGGAVIHTLREEIGDEAFRKAINIYLNRHKFENVETPDLKKAMEEASGRDL
                     TWFFNQWVYQGGFPKLEIRQVYQPRRKRLNLTVRQTQKADEITPAAFILPLEIEIAAN
                     GATKTEKIKIDKRTQN"
BASE COUNT          442 a          414 c          392 g          358 t
ORIGIN      
        1 accacttacc gctgaccact tttcaagggt ttatgaaatg tcgaaaactt tgtcctgccg
       61 cgttctttgt tctcgttttt gttttaccgc aatttgctca aacaaaaaat ttcaatcgcg
      121 cgcgcacttt cgacgttcag cattatacga ttcgcaccgc gttcgagcgt tcgaccaaaa
      181 ccgttttcgg cgacacgagc atccgcctga aaccgctcaa agccaacctg acgacggtcg
      241 agctcgacgc cgccggcttt aagctcgatt tcgtgcggct ggaagattcc ggcgtaaatc
      301 tgcaataccg tcaggctggc gaaaaactta ttatcacgct cgacaaagcc tatgcgccga
      361 aggactcgat ttcgattcgc atcggttatt cgtgccagcc gcagcagggc gtttatttcg
      421 tcgacgcttt aactgaaaaa aataaaatca tccgcgacgc gcaaatctgg acgcagggcg
      481 agccggaaga agcgcaccac tggtttccgt cttttgattt tccggacgat aaagcgacga
      541 ccgaacaatt tttgaccgtc gaggcgggcg aaacggcgat cgccaacggc gaattgctcg
      601 agactctgga aaactcgaac ggcacgaaaa cttttcacta taaaataagc gttccgcatt
      661 cggtttatct gacgtcgttt gtcgtcggaa agtattcgaa ggtcgccgat tcctacaaaa
      721 atattccgct cggtttttac gtttatcccg gacgagaagc aaccgcgcgc aaggctttcg
      781 gcaaaacgaa aaatatgatg cggatttttg aagattacac gggcgtggct tttccctaca
      841 gcaaatacga ccagacggtc gtcgccaatt tccaattcgg cggtatggaa aacatcaccg
      901 cgacgacgca cgccgacacc gaaattttcg ccgtcgatta tatgccggac gaagccgaag
      961 acctcgtctc gcacgagctg gcgcattcct ggttcggcaa tctggtcacc tgccgcaatt
     1021 gggcggaact gtggctgaat gaaggcttcg caacatttat ggaagccgct taccgcgaaa
     1081 aagcttacgg gcgcgccgat tatatgcgaa aaataaaaga cgacgcgacg cggttcataa
     1141 tcgacgaagc cgtcaataaa aaccgtcacg ggcttttcaa ccggctcgcg cgcccggaca
     1201 attcgatttt cgataccacg aattaccaga aagggggcgc ggttattcac acgctgcgcg
     1261 aggaaatcgg cgacgaagct ttccgcaaag cgataaatat ttatctgaac cggcacaaat
     1321 tcgaaaacgt ggaaacgccg gatttgaaaa aagcgatgga agaagcttcc ggtcgggatt
     1381 taacctggtt ttttaatcaa tgggtttatc agggcggttt tccgaaactc gaaatcagac
     1441 aggtttacca gccgcgcaga aaaagattga atttgaccgt caggcaaacg cagaaagccg
     1501 acgaaatcac gcccgcggct ttcattctgc cgttggaaat cgaaatcgcc gcaaacggcg
     1561 cgacgaaaac cgaaaaaatt aaaatcgata aaagaacgca gaattt
//
LOCUS       DASWNE010000420         5817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_683949, whole genome shotgun sequence.
ACCESSION   DASWNE010000420 DASWNE010000000
VERSION     DASWNE010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5817
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683949"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VGB68_08435"
     CDS             <1..289
                     /locus_tag="VGB68_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049296.1"
                     /translation="ERLVGAIKLRDVGAADIKDLLRSRSVRFVIADIGKPFEFIEDNE
                     IYNFWKTEVRIHLAEPESTTFLEDFPNHYCYFASEWKSFDGETIILLSKMH"
     gene            434..1045
                     /locus_tag="VGB68_08440"
     CDS             434..1045
                     /locus_tag="VGB68_08440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0049297.1"
                     /translation="MITANALTLENLRTLTDGELIAKALSGREDGFEELVRRYQRPIT
                     SYVYRMLGNYDASLDVTQEIFIKVYNSMARYSSEYKFSTWLYKIAHNASIDYLRRNSV
                     NQQSLEAENKDGSYELQIESKRLSPEQERERSEWRTEIESVVKCLPAGYRELIVLRHS
                     QDLSYDEIAEITNLPLGTVKNRLFRAREMMRDMFIERGFTGIT"
     gene            1060..1434
                     /locus_tag="VGB68_08445"
     CDS             1060..1434
                     /locus_tag="VGB68_08445"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HEY0049298.1"
                     /translation="MSYMQYQPNMSLQTPEQKTMGLIMHLSQLANLILFPVGIAIPIV
                     IWQTQKEKTPALDAHGKMVTNFMISMTIYFIVSFILVFVLIGFLLLPIVAIIGIVFPI
                     IGGIKANNGELWEYPLAIKFLK"
     gene            1463..2050
                     /locus_tag="VGB68_08450"
     CDS             1463..2050
                     /locus_tag="VGB68_08450"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY0049299.1"
                     /translation="MKEQAGKIEQTCPRADIAVYVDGELSPHQELVLEQHLAACETCR
                     AELNEQKKLLCALDFLLEEKASDIEIPANFAKVVATRAESHVSGLRNREERRRAVFLC
                     ASLGLIAMVGLGAEAKTVFAASGAVFEQILALTVFVAHLAYDVAFGVVVILRSLANQT
                     IVSAALVVAFLVGFWLVSWFAFSRFRQSSVSHVKK"
     gene            2047..3003
                     /locus_tag="VGB68_08455"
     CDS             2047..3003
                     /locus_tag="VGB68_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049300.1"
                     /translation="MIYIAAFKNPSGFQLPFRQIFAGLFCAFLFTVSLSAQSNISLVD
                     ENTLVVEDAKDMEIYSFGKKVIVKGEAKGVLAFGGDVIVEGRVGGDVATIGGSIYQKE
                     GAYIGGDVITFGGAYKHDSAEPLRNAEKESIVFAGYEDELREMMQNPSQIFAPQWSWT
                     FLAQRLLSALFWFIVSLGLTTIAPGAVSRAVARFQLSTAKVIGLGLLAGVISTIGVII
                     GLKFLPGYVGTITGLMVFLMLMLAYVFGRVSLQMSAGKWLQKMIMPEKRHSESVALLF
                     GVLFFTALLSLPYVWTLTVFVLWTASLGLVLTARATNNWQKI"
     gene            3279..4460
                     /locus_tag="VGB68_08460"
     CDS             3279..4460
                     /locus_tag="VGB68_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049301.1"
                     /translation="MYKTLAITAFFILFASANLTAFAQDAPKPTYFQGEVVSVTGGKL
                     NLQTKEGAVEITLQSTTAYKKVSPEKPRIADATDATLADIGAGDKVIVSSIVPADKKS
                     IPARAVYLMTKSDISKRNEAERTAWQTRGISGRVVSIDVPNKKIVVAMRGIGGERNVT
                     VTPKEIIEYRRYSPESVKFSDAKASSFGELSIGDQVRALGDRSADGTSFEAEKIVSGS
                     FKMVGGTITAIDAEKKEITIKDIQTNKPVTIVVNDASILKNFPPDVAQMMVTRMSGGG
                     ITPPGGGQGGGNVSVRPPNQGGNQQNPQGGGGQTRGGRGEFDDMIERFPSISLADLKV
                     GAAVAASSTNSVTPGRVTAIKLLSGVEPFFAAAAQAPRGGNNQSSPSLNIPGLDGIGT
                     P"
     gene            4569..>5817
                     /locus_tag="VGB68_08465"
     CDS             4569..>5817
                     /locus_tag="VGB68_08465"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY0049302.1"
                     /translation="MKHFLSLFFIGLLTLFISSSALAQQTGTLSGQVVDTLGAVVVGA
                     SVTAIDASGKEKSVVTNRLGEYSITGLAPGKYTIRTTAPTFQSYENTEVEIAAGQRQE
                     LVIALVAAGIEETVEVGNDGQVSTDQDNNASATVLKDKDLDALPDDPDELEAALQALA
                     GPSSGPNGGQFYIDGFTGGRIPPKESIREIRINQNPFSAEYERLGFGRIEILTKPGSD
                     KWRGSAFFNFNDESLNSRNPFSINRAASQTRFFGGNVSGPIQKGKSSFFLDVSNRDIR
                     NGSLVNATILDPSLGIIPFQQEFTVPTSRLSVSPRFDYQINTNNTLVARYSFTRNSSE
                     NQGISGFSLPSRAFETSNTSHEFRLTETMIVNPKTINETRFQYEWQKREQLGDNSIPT
                     VQVAGSFNGGGAQIGQNFTDDRRW"
BASE COUNT         1388 a         1438 c         1565 g         1426 t
ORIGIN      
        1 cgaaaggctg gtcggcgcca tcaagcttcg cgatgtcggg gcggcggata taaaggactt
       61 gctgcgctcg cgttcagttc gatttgtgat cgccgatatc gggaagccgt ttgaatttat
      121 agaggataat gaaatttata atttctggaa aacggaagtc agaattcatc tcgccgagcc
      181 tgaatcgacg acgtttttgg aagatttccc gaatcattac tgctattttg cttccgaatg
      241 gaaatctttc gacggtgaaa cgataattct tttgtccaag atgcattgaa attgcgcctc
      301 gaagtcccat taatttccgc catcaaaaat taaaatgcaa tgcggcaaag gaaaagattt
      361 tctttttgcc tttttgcttg tatagttaat tttttgaaac aacctttaaa atgattcgta
      421 atatctttgc gtcatgatta cagccaacgc actaacgcta gaaaatttac gcactttaac
      481 cgacggcgag ctgattgcga aagcgctttc cggtcgcgaa gacggttttg aggaactcgt
      541 gcgccgctac cagcgcccga ttacgtccta cgtgtatcga atgctgggca actacgacgc
      601 ttcgctcgac gtgacgcagg agatatttat caaggtttac aattcgatgg cgcgctacag
      661 ctcggaatat aaattttcga cctggcttta taaaatcgcg cacaatgctt cgattgatta
      721 tctgcggcgc aattccgtca atcagcaaag cctcgaagcg gagaacaagg acggaagcta
      781 tgaattgcag attgagagca agcggctttc gcccgaacag gaacgcgagc gcagcgagtg
      841 gcgaacggaa atcgaatcgg tcgtcaaatg tctcccggcg ggctatcgcg agttgatcgt
      901 cctgcgtcac tcgcaggatt tgagctacga tgaaatcgcg gaaatcacga atctgccgct
      961 cggaacggtg aaaaatcgcc tttttcgcgc gcgcgagatg atgcgcgata tgtttatcga
     1021 gcgcggcttt accggaataa cttaaaagga gaaaaacgaa tgagctatat gcagtatcag
     1081 ccgaatatgt cgctgcaaac gcccgagcaa aagactatgg gacttattat gcacctgtcg
     1141 cagcttgcca acctgattct ttttccggtc ggaatcgcca ttcccatcgt catctggcag
     1201 acgcagaagg aaaaaacgcc cgcgctcgac gcgcacggca aaatggtgac gaattttatg
     1261 atttcgatga ccatttattt tatcgtcagc tttatcctgg tttttgttct catcggattt
     1321 ctgctgcttc cgattgttgc tattatcgga attgttttcc cgattatcgg cggcatcaag
     1381 gcaaataacg gagagctttg ggaatatcct ctggcgatta aatttttgaa atagaaattg
     1441 ctgtctgctg agcgctataa aaatgaaaga gcaagccggt aaaatcgaac aaacctgccc
     1501 gcgcgcggac atcgccgttt atgtcgacgg cgaattgtcg ccgcaccagg aacttgttct
     1561 ggaacagcat ttagcggctt gcgaaacgtg ccgcgcagag ctgaacgagc agaaaaagct
     1621 tctctgcgcg ctggattttt tgcttgaaga gaaggcttcc gacattgaaa ttcccgcaaa
     1681 tttcgccaaa gtcgtcgcga cgcgcgccga aagtcacgtc agtggcttgc gcaatcgtga
     1741 ggagcggcgg cgagcggttt ttctgtgcgc ttcgctcggt ttgattgcaa tggtcggact
     1801 cggcgcggaa gcgaaaacgg tttttgcggc ttccggcgca gttttcgaac agattctggc
     1861 gcttacggtt ttcgtcgcgc atttagccta tgacgtcgct ttcggcgtcg tcgtcatatt
     1921 gcgctcgctg gctaatcaga caatcgtcag cgcggcgctg gtcgtcgcgt ttctcgtcgg
     1981 tttttggctc gtttcgtggt ttgcgttctc gcggttccgg cagtcgtccg tatcgcacgt
     2041 gaaaaagtga tttatatcgc agcctttaaa aatccctccg gttttcagct tccgtttcgc
     2101 caaatcttcg cgggactctt ttgtgcgttt cttttcaccg tttcgctctc tgcccaatcg
     2161 aatatttcgc tggtcgatga aaatacgctg gtcgtcgaag acgcgaaaga tatggaaatt
     2221 tattctttcg gcaaaaaagt catcgttaaa ggcgaagcga aaggcgtgct ggcattcggc
     2281 ggcgacgtga ttgtcgaagg acgcgtcggc ggcgatgtgg cgaccatcgg cggctcgatt
     2341 tatcaaaaag aaggagccta tatcggcggc gacgtgatta cgttcggcgg cgcgtataaa
     2401 cacgattccg ccgagccgct gcgcaacgcg gaaaaggaaa gcatcgtttt tgcgggctac
     2461 gaggacgaat tgcgcgaaat gatgcagaat ccatcgcaga tttttgcgcc gcagtggtcg
     2521 tggacgtttc tcgcccagcg tcttttatcg gctttgttct ggtttatcgt atcgctcggt
     2581 ttgacgacga ttgcgcccgg cgccgtcagc cgcgccgtcg cgcgatttca gctttcgacc
     2641 gcgaaagtca tcggcttggg cttattggcg ggagtcatca gcaccatcgg cgtgataatc
     2701 ggtctgaaat ttctgcccgg ctacgtcggc acgattaccg gcttgatggt ttttctgatg
     2761 ctgatgctgg cttatgtttt cgggcgcgtt tccctgcaga tgagcgccgg caaatggctg
     2821 cagaaaatga ttatgcccga aaaacgccat tccgagtcgg tcgcgcttct tttcggcgtc
     2881 ttgtttttta ccgctctgct ctcgctgcct tacgtctgga ctttaacggt ttttgtgctc
     2941 tggacggcta gtctggggct ggttctgacc gcccgcgcga caaataactg gcagaaaatc
     3001 taagccggac aatttttaag ttttgagtgt ggaattttga gttttcaatg gaatttaccg
     3061 aattcaaaac tcaaaattcc acactcaaaa ctttttctta taatccgtct gttaataatt
     3121 tagtaattcg ttcgttatta tttacaaaac tttacatagt tgtgaaacca acggcttgta
     3181 aaatgcgtct ttaaattaag ggcaaccttt aaccctataa gagcgtataa gttttggtaa
     3241 taattctttt atccgttcaa ggaagtaaaa aatcagaaat gtataaaaca ttagcaatta
     3301 cggctttttt tattttgttc gcgagcgcga atttgacggc ttttgcgcag gacgcgccga
     3361 agccgactta ttttcagggt gaagtggttt cggtgaccgg cggcaaactc aatttgcaga
     3421 ccaaagaagg cgcagtggaa atcactctgc aatcgacgac cgcttataaa aaagtgtcgc
     3481 cggaaaagcc gagaattgcc gacgcgaccg acgcgacgct cgccgacatc ggcgcgggcg
     3541 ataaggttat cgtctcgagc atcgttccgg cggacaaaaa atctattccc gcgcgcgcgg
     3601 tgtatctgat gacgaaatcg gacatttcca agcgaaacga agccgagcgc accgcctggc
     3661 agacgcgcgg aatatcggga cgcgtcgttt cgatcgacgt cccgaataaa aaaatcgtgg
     3721 tggcgatgcg cggcatcggc ggcgaaagaa acgttaccgt aacgccgaaa gaaataatcg
     3781 aatatcggcg ctactcgccg gagtcggtca aattttccga cgcgaaagcc agcagcttcg
     3841 gcgagctttc catcggcgac caggttcgcg cgctcggcga tagaagcgcg gacggaacga
     3901 gctttgaagc ggaaaaaatc gtctccggct cgtttaagat ggtcggcgga acgattaccg
     3961 ccatcgacgc cgagaaaaaa gaaatcacga ttaaagacat tcaaacaaat aagcccgtca
     4021 ccatcgtcgt caacgatgct tcgatactga aaaattttcc gcccgatgtg gcgcagatga
     4081 tggtgacgcg gatgtcgggc ggcggaatta cgccgccggg cggcggtcag ggcggcggaa
     4141 atgtctcggt gcgcccgccg aatcaaggcg gaaatcaaca aaatccgcag ggcggaggcg
     4201 gtcagacgcg cggcggtcgc ggcgaatttg acgatatgat cgagcgtttt ccgagtatct
     4261 cgctggcgga tttaaaagtc ggcgcggcgg tcgccgcttc gagcacaaac agcgtgacgc
     4321 cgggtcgcgt gacggcgatt aagcttcttt cgggcgtcga gcctttcttt gccgcggcgg
     4381 cacaagcgcc gcgcggcgga aataatcaaa gctcgccgag cctgaacatt ccgggcttgg
     4441 acggaatcgg tactccgtaa aaagtttttt tgaaaccgcg ccggctgcgc attttatttt
     4501 attttattct tcggcagcca aagcgcggtt tcatttttct ggattttaga aatgcacggg
     4561 caattattat gaaacatttt ttatctttat tttttatcgg tcttcttact ttatttataa
     4621 gttcgagcgc gctggcgcag cagacgggaa ctctgagcgg tcaggtcgtc gatacgctcg
     4681 gcgcggtcgt ggtcggcgcg agcgtgaccg ccattgacgc gagcggcaaa gaaaaatcgg
     4741 tcgtaaccaa tcgtctgggc gaatattcga ttaccggact ggcgccgggc aaatacacga
     4801 ttcgcacgac cgcgccgact tttcagtctt acgaaaatac ggaagtcgaa atcgcggcgg
     4861 gtcagcggca ggaactggtt atcgccctgg tcgcggcagg aatcgaggaa acggtcgaag
     4921 tcggaaacga cgggcaagtc agtaccgacc aggacaacaa cgcgagcgcg acggttctca
     4981 aggacaagga tttagacgct ctgccggacg acccggacga gctggaagcc gctttgcagg
     5041 ctttagccgg accttcttcc ggaccgaacg gcggtcagtt ttacatcgac ggctttaccg
     5101 gcgggcgcat tccgccgaag gaatcaatcc gcgaaatacg catcaatcaa aatccgtttt
     5161 cggcggaata cgagcggctc ggtttcggca gaatcgagat tttgacgaaa cccggctcgg
     5221 acaaatggcg cggcagtgct tttttcaact ttaacgacga aagcctgaac tcgcgtaacc
     5281 cgttttcgat aaaccgcgcg gcgagccaga cgcgcttttt cggcggaaac gtttcgggtc
     5341 cgattcagaa agggaagtct tccttttttc tcgacgtcag caaccgcgac atcagaaacg
     5401 gttcgctggt caacgcgaca attctcgacc cgtcgctcgg catcattccg tttcaacagg
     5461 aatttaccgt gccgaccagc cgtctgtccg tcagcccgcg tttcgattac cagataaaca
     5521 cgaacaacac gctggtcgcg cgctacagct ttacgcgcaa cagctcggaa aatcagggca
     5581 tcagcggttt ttcgctgccc tcgcgggcgt ttgagacgtc gaacacctcg cacgagtttc
     5641 gtttgaccga gacgatgatt gtcaatccga aaacaatcaa cgaaactcgc tttcaatacg
     5701 aatggcagaa acgcgagcaa ctgggcgaca attcgattcc gaccgttcag gttgcggggt
     5761 cgtttaacgg cgggggcgcg caaatcggac agaattttac cgacgaccgg cgctggg
//
LOCUS       DASWNE010000421         1790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_14547, whole genome shotgun sequence.
ACCESSION   DASWNE010000421 DASWNE010000000
VERSION     DASWNE010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14547"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VGB68_08470"
     CDS             <1..405
                     /locus_tag="VGB68_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEY0049303.1"
                     /translation="NIMSTKIYVGNLSFNTSTQDLEDMFGEFGTVQSTNIIEDRETGR
                     SRGFAFVEMSSNEEAQAAITALNGKDMDGRSLTVNEAKPREEGGGRGGNRGGGGGGRG
                     GYGGGGGGNRGGGGGGGNRGGGGGYGGGGSRW"
     gene            549..767
                     /gene="infA"
                     /locus_tag="VGB68_08475"
     CDS             549..767
                     /gene="infA"
                     /locus_tag="VGB68_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEY0049304.1"
                     /translation="MKEELIEAAGLIIDKQPNAFFKVQINGSEHIVLATISGKMRKNR
                     IRILIGDRVDVEMSPYDLSRGRITYRHK"
     gene            complement(812..955)
                     /locus_tag="VGB68_08480"
     CDS             complement(812..955)
                     /locus_tag="VGB68_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049305.1"
                     /translation="MKTFYAPICKSPSGSRPDKGKRPGKLTIAGAFSVLTFIFEVKIV
                     PEF"
     gene            989..>1790
                     /locus_tag="VGB68_08485"
     CDS             989..>1790
                     /locus_tag="VGB68_08485"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0049306.1"
                     /translation="MSETFQLPSLEIEEYTLGNGLRVVLNRDESVPVVSCAVYYDVGS
                     RNEREGRTGFAHLFEHMMFQGSENVPKAGHFQFIMKAGGTMNGTTSSERTNYYETLPA
                     SQLPLALWLESDRMRTLAVTQENLDNQRDAVKEEKRLRYDNQPYGQVFDLLTSMVYKN
                     FANAHSTIGSMEDLDAATVDDVQEFFRVYYAPNNAVLVLSGSFDAAEAKSLIEKYFAT
                     IPSQPKPAALDVSEPPEISLSAHTYVDKLAPFPAFIDGWKIPTRRTPKF"
BASE COUNT          457 a          433 c          491 g          409 t
ORIGIN      
        1 aatattatgt caacgaaaat ttatgtagga aatctttcct tcaacacatc aacacaggat
       61 ttagaagata tgttcggcga gttcggcacg gttcagtcaa ccaacattat cgaagaccgc
      121 gaaacgggtc gctcgcgcgg ctttgcgttt gtcgaaatgt cgagcaacga agaagcgcag
      181 gctgcgatta ccgcgcttaa cggcaaagat atggacggtc gttcattgac ggtcaacgaa
      241 gccaagccgc gtgaagaagg cggcggacgc ggcggcaatc gcggcggcgg cggcggcgga
      301 cgcggcggct acggcggcgg cggcggtgga aaccgcggcg gcggtggcgg cggcggcaat
      361 cgcggcggcg gcggcggcta cggcggcggc ggttcgcgct ggtagaagcg tcacgcttaa
      421 ttaataagcg agattgaaat gtgcctcggg ctaaaagatt agcccggggc gctaattttt
      481 tccgagcaat atcttttatc agttttgctc aaaccgaatc agccgacaat caaaggaagt
      541 gattttaaat gaaagaagaa ttaatcgaag cggcgggact tattatcgac aaacaaccta
      601 atgctttttt taaagtgcaa atcaatggca gcgaacatat cgttctggca acgatttcag
      661 gcaaaatgcg gaaaaatcgg attcggattt tgatcggcga ccgcgtcgat gtcgaaatgt
      721 cgccctacga tttatcgaga ggcagaatca cctaccggca caagtaaaaa gctctcgaac
      781 ttataaaaaa tagtgatata aaaaaatccg gttaaaattc cggcacgatt ttaacctcga
      841 aaataaatgt taaaacagaa aacgctccgg caatagtaag cttgccgggg cgttttcctt
      901 tgtctggtct gctgccggac ggacttttgc aaattggcgc gtaaaatgtt ttcataaatt
      961 taagcttttt aagcgggatt tcagtattat gtcagaaact tttcaacttc cgtctttaga
     1021 aatcgaagaa tatacgctcg gcaacggctt gcgcgtggtt ttaaaccggg acgagtccgt
     1081 tccggtcgtg tcgtgcgccg tttattatga tgtcggctcg cgcaacgagc gcgaagggcg
     1141 cacgggtttt gcgcatcttt tcgagcatat gatgtttcaa ggctcggaaa acgtgccgaa
     1201 agccggacat tttcagttta ttatgaaagc cggcggcacg atgaacggga cgacttccag
     1261 cgagcggaca aattattacg agactctgcc cgccagccag ctgccgctcg ctctgtggct
     1321 ggaatccgac cggatgcgaa cgctcgccgt cacgcaggaa aatctcgaca accagcgcga
     1381 tgccgtcaaa gaagagaaac gactgcgtta cgacaatcag ccgtatggtc aggttttcga
     1441 tttgctgacc tcgatggttt acaaaaattt cgccaacgcg cattcgacca tcggctcgat
     1501 ggaggatttg gacgcggcga cggtcgacga cgtacaggaa tttttccgcg tttattacgc
     1561 cccgaacaac gccgttttgg ttctttccgg ctcgtttgac gccgccgagg cgaaatcttt
     1621 aatcgaaaaa tatttcgcga cgattccgag ccagccgaag ccggcggcgc tggacgtttc
     1681 cgagccgccg gaaatcagct tgagcgcgca cacttatgtc gataagctcg cgccttttcc
     1741 ggcgtttatc gacggctgga agattccgac ccgccggacg ccgaaatttt
//
LOCUS       DASWNE010000422         1825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_439522, whole genome shotgun sequence.
ACCESSION   DASWNE010000422 DASWNE010000000
VERSION     DASWNE010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439522"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..1271
                     /locus_tag="VGB68_08490"
     CDS             222..1271
                     /locus_tag="VGB68_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049307.1"
                     /translation="MKRVGILVGREQTFPESIIKSINEKSGGEITAEMVKVGGIRLDE
                     QKRYDLIIDRISHEVPYYRATLKRFALEGTYIVNNPFWWSADDKFFNFCLGAKLGVAI
                     PKTVLLPQEKYIPDITSESLRNLEFPIDWQGIVDYVGFPAFLKPFDGGGWKNVSKIHS
                     LEELWHEYNQTGTLCMTLQEGIEYDQFVRCYCVGQEKVLIMPYDPSKPYLSGMQYVNV
                     EDYLSPELHARVEKDVITLCKALGYDLNTVEFAIKDGIPYAIDFMNPAPDAELASVGE
                     YNHNWIVNAMTDFIINKLNGEIAPPEYRWTAMLSGENETAPKAAAKRAPKAAKTKIET
                     GAETKSRSKKASNAE"
     gene            complement(1368..>1825)
                     /locus_tag="VGB68_08495"
     CDS             complement(1368..>1825)
                     /locus_tag="VGB68_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049308.1"
                     /translation="RARRVFQKALDATHESFRSVRGLAEIALREGKIAHVIHHFATAI
                     HFADTKALRNWAKSETDYFSRLNTDEEYMEQEIRRINWLEGILRGKKMALRFAMIGFI
                     AILLGLLTEDIVTNIGWTVSAVSLLIWVGLIMSGNLLAERSSLLEAAED"
BASE COUNT          485 a          476 c          446 g          418 t
ORIGIN      
        1 cggttgagtt atttgtttat ttttataacg agcgacaact cagaaaaaga cgtttaccga
       61 aatatccggc ttatctggcg caacatctgc caacacttat ttagtcactc ccgtaatcct
      121 tgcgatgatt tggctaatca attaaaatta acggtttcag acgcgtctga aaatctaagc
      181 aattatctac tatcaatttt taaaaaggaa ttcagcacat tatgaaaaga gttggaattt
      241 tagtcgggcg cgagcagacg tttcccgaat cgattattaa aagcatcaat gaaaaatcgg
      301 gcggcgaaat cactgccgaa atggtcaaag tcggcggcat tcgcctcgac gagcaaaagc
      361 gctacgattt aatcatcgac cgcatttcgc acgaagttcc gtattatcgc gcgaccttaa
      421 agcgtttcgc gctggaaggc acctatatcg tcaacaaccc gttctggtgg tcggctgacg
      481 ataaattttt taatttttgt ctgggcgcca aactcggcgt ggcgattccg aaaaccgtcc
      541 tgctgccgca ggaaaaatat attcccgaca ttacgagcga atctctgcgc aatctcgaat
      601 ttccgattga ctggcagggc atcgtcgact atgtcggctt cccggctttt ctgaaaccct
      661 tcgacggcgg cggctggaaa aacgtctcga aaattcattc gctggaagag ctctggcacg
      721 aatacaacca gaccggaacg ctttgtatga ccttgcagga aggcatcgaa tacgaccagt
      781 tcgtgcgttg ttactgcgtc gggcaggaaa aagttttgat tatgccgtac gacccttcga
      841 agccttacct gtcgggaatg cagtatgtaa acgtcgaaga ttatctttcg ccggagctgc
      901 acgcgcgcgt cgaaaaagac gttattacgc tgtgcaaagc actcggctat gatttgaaca
      961 cggtcgaatt cgcgattaag gacggcattc cgtatgcgat tgactttatg aatcccgcgc
     1021 cggacgccga gctggcatcg gtcggcgaat acaatcacaa ctggatcgtc aacgcgatga
     1081 ccgattttat tatcaacaag ctgaacggcg aaatcgcgcc gcccgaatat cgctggacgg
     1141 caatgctgag cggcgaaaat gaaaccgcgc cgaaagccgc cgccaaacgc gcgccgaaag
     1201 ccgcgaaaac aaaaatcgaa accggcgcgg aaacgaaatc gcgctcgaag aaagcgtcca
     1261 acgcggaata gcgaccaacg gggaatagag gcgcggcgaa acagtgacgc ggcgacgcgg
     1321 ggaaataatc tccgtgtcgc cgcgtctcat tttctccgcg tcataactta atcttccgcc
     1381 gcttcaagca gcgagcttcg ctccgccaga agatttccgc tcattatcaa gccgacccaa
     1441 atcaacaggc tcaccgccga caccgtccag ccgatattgg tgacaatatc ttcggtcagc
     1501 aaaccgagca aaatcgcgat aaaaccaatc atcgcaaagc gcagagccat ttttttgccg
     1561 cgcaggattc cttccagcca gttgatgcgg cggatttcct gttccatata ttcttcgtcc
     1621 gtgttcaggc gcgaaaaata atcggtctcg cttttcgccc agtttctcaa agctttcgtg
     1681 tcggcaaaat gaatcgcggt cgcgaaatga tgaatgacgt gcgcgatttt tccttcgcgc
     1741 agagcgattt ccgccaagcc gcgaacggaa cgaaaacttt cgtgcgtcgc gtccagagcc
     1801 ttctggaaaa cgcgacgggc gcgtt
//
LOCUS       DASWNE010000423         2118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_262252, whole genome shotgun sequence.
ACCESSION   DASWNE010000423 DASWNE010000000
VERSION     DASWNE010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262252"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VGB68_08500"
     CDS             complement(<1..562)
                     /locus_tag="VGB68_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049309.1"
                     /translation="MKKKLRIAINAQIPLGSGTGGIETVLRALTALGRLGDGDEEYVF
                     IGHWSDPDWLKPFLGERQTIVSAPRPQENKPTAAENFKRSLGRLRPLAGKVKRAFAPQ
                     SRVQSPPTRICVPVSDGFYENLGCDVIHFPYQDYVYCRVPTVYNPHDLQHLHYPDFFS
                     PEEIERREIIYPAACRAAGTVVVASEF"
     gene            complement(725..>2118)
                     /locus_tag="VGB68_08505"
     CDS             complement(725..>2118)
                     /locus_tag="VGB68_08505"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HEY0049310.1"
                     /translation="VNLYFHYQYVPEPLTPVKGVRKLDAAHLLTVDVENWRIEEKKYW
                     RMEDAPPLDGEPGELIREKLEEVSELIVRSDVPVGVALSGGLDSSVVAAFAARRQNSL
                     CAFSVGYAGRPDCDEREDAAALARHLKLPFHEIELDVRTIVDFFPQLNYWRDDPVADI
                     AGFGYYAVMKLARERGVPVLLQGQGGDELFWGYPQLRDAARETHLKAALLDSPLSQAL
                     RQALTLRLPYYRSEQTVSSWARDLAGLRSGWRGVRRRRENPARMIFYDLSPDFAAAAA
                     RMRNFYGRNFVEQINERDASEIFTFPGGWSPVETTLTKLICDTYLRENGIAQGDRLGM
                     ASSVEMRLPLVDYKFVETVVGLRKRHSDSTLPPKFWLRQAVKDVLPEWVLNRPKRGFA
                     PPTRRWHDALFAAYGESLRDGYLVESEVLNRESAARLAEGEFPPEATSPLSFKALVLE
                     QWCRQMRRVIPAI"
BASE COUNT          496 a          627 c          569 g          426 t
ORIGIN      
        1 cgaattcgga agcgacgacg accgtcccgg cggcgcggca cgcagccgga taaattatct
       61 cgcgtctttc aatttcctcc ggcgaaaaaa aatcgggata atgaagatgc tgtaaatcgt
      121 gcggattata aacggtcgga acgcggcaat agacataatc ctggtaagga aaatgaatca
      181 cgtcgcagcc gagattttcg taaaatccgt ccgaaacggg aacgcaaatc cgagtcggcg
      241 gagactgcac gcgtgattgc ggcgcgaaag ctcgtttgac tttgcctgct aacgggcgca
      301 gccgaccgag cgagcgttta aaattttcgg cggcggtcgg tttattttcc tgcgggcgcg
      361 gcgcggaaac tatcgtctgg cgctcgccca aaaacggctt cagccaatcc gggtcagacc
      421 agtgaccgat aaaaacatat tcctcgtcgc cgtcgccgag ccgccccaaa gcggtcaggg
      481 cgcgcagcac cgtttcgatg ccgcccgtgc cgctgccgag cggaatctgc gcgttgatgg
      541 cgatgcgtaa ttttttcttc aaagttatat ctatgactgc cgggaaaatg aaaccacgga
      601 taacacggat aaacacggga tattcaaaaa gaataattat tttgattgat ttatcatcct
      661 agttaatccg tgtaaatctg tggtttcata cctttcttaa atttagtccg cctgaaagtt
      721 taaattaaat tgccggaata acacgccgca tttggcgaca ccactgttcc agaacgagcg
      781 ccttgaaaga cagcgggctg gtcgcctcgg gcggaaattc cccttcggcg agccgcgccg
      841 cgctttcgcg atttaaaact tccgactcga ccagataacc gtcgcgcaaa gattcgccgt
      901 aagcggcaaa aagcgcgtcg tgccaacggc gcgtcggcgg agcgaaaccg cgtttcggtc
      961 gattcaaaac ccattccggc aaaacgtctt taaccgcttg cctgagccag aattttggcg
     1021 gaagcgtcga atccgaatgt ctttttcgca agcccacaac cgtttcgaca aacttgtaat
     1081 cgaccagcgg aagccgcatc tcgaccgaag aagccattcc gagccggtcg ccctgcgcga
     1141 tgccgttttc gcgcaggtat gtgtcacaaa ttaatttagt cagagtggtc tccaccggcg
     1201 accagccgcc gggaaaagta aatatttcgc tcgcgtcccg ttcgttaatc tgttcgacga
     1261 aattcctgcc gtaaaaattc cgcatccgcg ccgcggcagc cgcaaaatcc ggcgataaat
     1321 cataaaaaat cattcgcgcc ggattctcgc gccgccgcct gacgccgcgc cagccggaac
     1381 gcagtccggc gaggtctcgc gcccacgaag aaacggtttg ctccgaacga taatacggaa
     1441 gacgcagcgt caaagcttgc cgcagcgcct gcgaaagcgg gctgtcgagc agcgcggctt
     1501 tcaaatgcgt ctcgcgcgcc gcgtcgcgca gctgcgggta gccccagaaa agctcgtcgc
     1561 cgccctgccc ttgcagcaga accggcacgc cccgctcgcg cgccagcttc atcacggcgt
     1621 aatagccgaa accggcgatg tcggcgacgg ggtcgtcgcg ccagtaattg agctgcggaa
     1681 aaaaatcgac aattgttcgg acatcgagct cgatttcgtg aaacggcaac ttcaaatggc
     1741 gcgccagcgc tgccgcgtcc tcgcgctcgt cacaatcggg tcgtccggca taaccgacgc
     1801 tgaaggcgca gagagagttt tgccgccgcg ccgcgaaagc cgccacgacg ctggaatcca
     1861 agccgccgct caacgcgacg ccgaccggaa cgtccgagcg cacgattaac tcgctgacct
     1921 cttcgagctt ttcccgaata agctcgcccg gctcgccgtc gagcggcggc gcgtcctcca
     1981 tccgccaata ttttttttct tcaattcgcc aattttcgac gtcgaccgtg agcagatgcg
     2041 cggcatcgag cttacgcact ccttttaccg gcgtcagcgg ttcgggcacg tactgataat
     2101 gaaaataaag attgacgg
//
LOCUS       DASWNE010000424         1992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_297268, whole genome shotgun sequence.
ACCESSION   DASWNE010000424 DASWNE010000000
VERSION     DASWNE010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297268"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            409..1497
                     /gene="nuoH"
                     /locus_tag="VGB68_08510"
     CDS             409..1497
                     /gene="nuoH"
                     /locus_tag="VGB68_08510"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEY0049311.1"
                     /translation="METAIKTIFPIAVYSVAVLLAFQGVLMIVAYTVLAERKVLGWIQ
                     GRIGPNRVGPWGVLQPFADLLKFIFKEDLVPDKSTKFVYFLAPLIALICALMPIIVYP
                     FGPAITTVDWKFLPYGLGENIQALPLTIANFDVAVLYVLGITSVGVYGLALAGWASNS
                     KYSLMGGLRSSAQMISYELAMGASVLGVVMMAGSLSLNGIINAQTSSWSKWYIIPQFI
                     GFIVFLIAAFAETNRVPFDLPEAETELVAGFHTEYSALKFALFFMAEYVNMFTVSVMC
                     VTLFLGGWYIPGLSHIFELGSIPYAIASHVGFLLKIFFFLFLYIWIRGTLPRFRFDQL
                     MNFGWKFLLPLAIANVIFTIVMVYLGVF"
     gene            1577..>1992
                     /locus_tag="VGB68_08515"
     CDS             1577..>1992
                     /locus_tag="VGB68_08515"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEY0049312.1"
                     /translation="MITPLFFLFAGLAIACAISLVYHKNPLYSAISLIGVLISLACIY
                     VTLAAPFIAAVQVLVYAGAIMVLVVFVIMLLNLEEDRPLNRLRYLYAVGAVLGLALLA
                     QTFFIFYAVTRTTGQTAMPEQGTGVTQNIGRAMYTE"
BASE COUNT          405 a          470 c          532 g          585 t
ORIGIN      
        1 ggcggaagcg gcggcggtcg gcgtcaaaga ccgtttggac gataaggaaa ccgttccgaa
       61 cacggtcgtt ctcagataat tttataatcg gtgttgtgca tattttggtc gcacggagct
      121 tgatacgcgt ttctatttta atatttgcta aatcaaatga gaacgcggag tattagaaga
      181 cataattcgg aaaagtttaa gcggcgatgc cggagattag cccgctaccg cttttcgctt
      241 tccgcttccg acgagtcggc aaacgatgtg aaatttgtcg ggcggctggc gcggacacgc
      301 aagccttgtt cttgttccat gtgcggcaac ccgcgaaaat attttaacga aaggacgata
      361 agagaacgtc gtcaagagtt agcaagtaaa taagttaact taatttttat ggaaactgct
      421 ataaaaacaa ttttcccgat tgcggtttac agcgtcgcgg ttttgctcgc gtttcagggc
      481 gttttgatga ttgtcgccta taccgttctg gcggaacgca aggttctggg ctggattcag
      541 ggacgcatcg gaccgaaccg ggtcggaccg tggggcgttt tgcagccgtt cgcagacctt
      601 ctgaaattca tctttaaaga ggatttggtt cccgataaat cgacgaaatt cgtttatttt
      661 ctcgccccgc tcatcgcttt gatttgcgcg ctgatgccga ttatcgttta cccgttcggt
      721 ccggcgatta cgaccgtgga ctggaaattt ttgccttacg gcttgggcga aaacattcag
      781 gcgctgccgc tgacgatcgc caatttcgac gtcgcggttt tgtatgttct gggaattaca
      841 tcggtcggcg tttacggact cgcgctggcg ggctgggctt caaactcgaa atacagcctg
      901 atgggcgggc tccgctcgtc ggcgcagatg atttcatacg agctggcgat gggagcctcg
      961 gtgctcggcg tggtaatgat ggcgggcagc ttgagcctga acggaattat caacgcgcag
     1021 acgtcttcct ggtcaaaatg gtatatcatt ccgcaattca tcgggtttat agtctttttg
     1081 attgcggctt tcgccgaaac caaccgcgtt ccgttcgatt tgccggaagc cgagaccgag
     1141 ctggtcgccg gttttcacac ggaatattcg gctttgaaat tcgcgctgtt ttttatggcg
     1201 gaatacgtca atatgttcac cgtctcggtg atgtgcgtga cgctgtttct gggcggctgg
     1261 tatattccgg gcttgagcca tatttttgaa ctcggctcga ttccctacgc catcgccagc
     1321 cacgtcgggt ttttattaaa gattttcttt ttcctgtttt tgtatatctg gattcgcgga
     1381 acgctgccgc gttttcgttt cgaccagttg atgaatttcg gctggaaatt tttgcttccg
     1441 ctcgccatcg ccaacgtgat ttttacgatt gtgatggttt atctgggagt attttaaacg
     1501 atgaatcagg aacgatgaac gatgaatttt ttctttcatt ccgcgttccg cgttccgcat
     1561 tcataatttg accgtaatga ttacaccgct tttctttctc tttgccggac tcgccatcgc
     1621 ctgcgcgatt tcgctggttt accataaaaa tccgctttac agcgcgattt cgctgatcgg
     1681 cgttttgatt tcgctggctt gtatttacgt gacgctcgcc gcgccgttta tcgccgccgt
     1741 gcaagttctg gtttacgcgg gcgcgattat ggttctggtc gttttcgtca taatgctttt
     1801 gaatcttgaa gaagacagac cgctcaaccg cctgcggtat ctctacgccg tcggcgcggt
     1861 tttgggcttg gctttgctgg cgcagacgtt ttttatattc tacgccgtca ccagaacgac
     1921 cggtcagacg gcgatgccgg agcaaggaac cggcgtcacg cagaatatcg gcagagcgat
     1981 gtatacggaa ta
//
LOCUS       DASWNE010000425         7134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_544879, whole genome shotgun sequence.
ACCESSION   DASWNE010000425 DASWNE010000000
VERSION     DASWNE010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7134
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544879"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /gene="trpS"
                     /locus_tag="VGB68_08520"
     CDS             complement(<1..735)
                     /gene="trpS"
                     /locus_tag="VGB68_08520"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEY0049313.1"
                     /translation="MKKRIFSGAQPTGELHIGNYLGALKNWVALQHEYESFFCIVNLH
                     AITLPQKPQVLKQKTLDLARIYLAAGIDPQVSTIFIQSDVAEHAELAWILNCVARMGE
                     LERMTQFKDKSKGKSERVGAGLFTYPVLMASDILLYQTDLVPVGQDQKQHVELTRDLA
                     ERFNRDYGETFRIPGDFIPPVGAKIQSLQEPAKKMSKSDENLNGSIFLLDDADAITKK
                     IKRAVTDSGTEIRFDAERPAINNLLTI"
     gene            complement(739..1419)
                     /locus_tag="VGB68_08525"
     CDS             complement(739..1419)
                     /locus_tag="VGB68_08525"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY0049314.1"
                     /translation="MNDFDIAQFVSQLVIYMVVWIFAVSLHEAAHAWVSYKFGDDTAY
                     QLGRVTVNPIPHIDPIGTLVLPILGFVLSYTSAFNFPLIAWGKPTPVNPLKWRNKDTA
                     NVMVSIAGIVVNLIIATVSAVIIKILIASQVITPDNFQVGLVSVLFNFLYTMVLMNVG
                     LAIFNLLPFPPLDGSKVLQTFLPRSFEPLFEILETYGFLILLVLLQLGIIRMIVVPLN
                     NLVLSLLF"
     gene            1559..1987
                     /locus_tag="VGB68_08530"
     CDS             1559..1987
                     /locus_tag="VGB68_08530"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY0049315.1"
                     /translation="MLKRILFGGETSASPLTNLGLTLLRIFAGIGLMTHGIGKIPPSE
                     KFIEGVTGMGFPAPQAFAWAAGLSEFVGGAFLILGLFTRPVSFFIACTMATALIGQHR
                     NDPFAVQEKAFLYLFIALAFLFMGANFWSVDGILRRNRSL"
     gene            2105..3328
                     /locus_tag="VGB68_08535"
     CDS             2105..3328
                     /locus_tag="VGB68_08535"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEY0049316.1"
                     /translation="MSKDKNYIVCIASEHKGNDFLEECQEAGWHVTLVTKEKLLDEPW
                     AWTSLNGVKTVPDEATTEDYIRAVTNVAGTQPVTRIVGLDEFDVLTAAKAREHLQVGG
                     GMTGSFALRFRDKLRMRRLAKHVDIPCPEFAGIINRAQIDEYLESAAAPWIIKPRTEV
                     SAFGIRKCETKQQVWQVLNELDARNTWRDHPSQFLIERFIEGRVFHVDSVVADGKIIA
                     SGVSEYGAPPMRVSHEGGVFTTALLPYKSKERKELEKLNKDLLKGFEYEQGVSHAEFL
                     QSAETGEFYLLEVAARVGGAYIANVLEQASGFNLWREWAKLETATKKNPYQPPKLRKD
                     YAGIALALSKEQTPDTSHYTDEEIVYRVTKPKHVGLIFFSKKHKRIEELLKTYTEKIT
                     RDFLAVAPVKERYDD"
     gene            3412..4650
                     /locus_tag="VGB68_08540"
     CDS             3412..4650
                     /locus_tag="VGB68_08540"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY0049317.1"
                     /translation="MTVETASQTSSPAILEHHLERVFPALLKFESTPEMIKSAVYQSV
                     KPEVERILNAVVEENFAPETFEKTDTARALAWNIERGNRFEGIVEALKNHPQLKDRDV
                     LLLTELDYGMARSGNRFVARELARQLKLNYAFAPVYIALQKGSGVESEVEGENTKSLH
                     GLAMFSKYPLKNAHAIPLPNGKDKMLGKEKRLGHLRALIADVRHPAGVFRAVTLHLDA
                     HSSRKHRQRQMRIVLEHLETLPELPTVIGGDWNTSTYNAQNATRAILGYWRRDMIGAK
                     RTVRNHFPHPDRYFEKGLFGELEKRGYDYKNLNNPGVGTLHYDVESIEKNTNLRDWVP
                     EWCFPFIFWAARRVGGRVSGRLDWFAGKGIKLTGKPQTIGNLYDAENIPLSDHDAIAL
                     DFAPSIRHKDTKDSKAIQIA"
     gene            4725..4976
                     /locus_tag="VGB68_08545"
     CDS             4725..4976
                     /locus_tag="VGB68_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049318.1"
                     /translation="MEVSFCKTTSFNADNFEYEIIKAENGYAEMIKFPIDETQNSPGD
                     VVVIIDGEDIQFHGIIGKIEEGYAFAYDPKGSTLPASVQ"
     gene            5025..5101
                     /locus_tag="VGB68_08550"
     tRNA            5025..5101
                     /locus_tag="VGB68_08550"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5059..5061,aa:Ala,seq:cgc)
     gene            5359..5526
                     /locus_tag="VGB68_08555"
     CDS             5359..5526
                     /locus_tag="VGB68_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049319.1"
                     /translation="MLNVDLLLELVIFILAGFLMIVAGGTFYVWWNGYHKPDSHTRQR
                     LHYKRHYRSRL"
     gene            complement(5515..5943)
                     /locus_tag="VGB68_08560"
     CDS             complement(5515..5943)
                     /locus_tag="VGB68_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049320.1"
                     /translation="MKRSNKPKTPIVAIDFDGTIVAHRYPEIGEPVPYALETMRAMQA
                     KGYKLILYTMRSGEYLQEAVDYAAAAGIEFWAVNENPAQRYWTDSPKVYAHIYIDDAA
                     FGCPLVKVENDRPIVDWLEVARHFELIEYISETKRAASES"
     gene            complement(6032..6166)
                     /locus_tag="VGB68_08565"
     CDS             complement(6032..6166)
                     /locus_tag="VGB68_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049321.1"
                     /translation="MKQIRKGRILIFLRALFSIEVSGQIKNIVGIEEITSALPAAGER
                     "
     gene            6301..6981
                     /locus_tag="VGB68_08570"
     CDS             6301..6981
                     /locus_tag="VGB68_08570"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY0049322.1"
                     /translation="MLRNLTLIAFCLIVSTGAASAQKKSTDTEVKPKTLKKSNQRPAG
                     KTPKAEPFEKATVEIMSAQCVKLETEAGVIELEMFPESAPESVRNFLNLAAIGAFDTT
                     TFSRVVPRFVIQGGNLTTREKLTPELDKRMRRTIPDEPNQIKHERGILSMARSDEPNT
                     ASTHFFILVDSAPSLDGTFAAFGRVTQGMEVVDAINKMPVEKDKPSKPVRITRASVAP
                     CAPQTTTK"
BASE COUNT         1834 a         1797 c         1800 g         1703 t
ORIGIN      
        1 aatcgtcaaa agattgttta tcgccggtcg ctcggcgtca aagcgaatct ccgttcccga
       61 atcggtgacg gcgcgcttga tttttttcgt aatcgcgtcc gcatcgtcca gcagaaatat
      121 cgagccgttc aggttttcgt ccgatttaga catttttttc gccggctcct gaagcgattg
      181 aatcttggcg ccgacgggcg gaataaaatc tcccggaatc cgaaacgttt cgccgtaatc
      241 gcgattgaat ctttcggcta aatcgcgcgt caattcgacg tgctgtttct ggtcctgccc
      301 gaccggaacc aaatcggttt gataaagcag gatatcggaa gccatcagca ccggataagt
      361 gaaaagcccc gcgccgacgc gctcgctttt gcctttcgat ttgtctttaa actgcgtcat
      421 tcgctccaat tcgcccatcc gcgcgacgca atttaaaatc cacgccagct cggcgtgttc
      481 ggcgacgtcc gattgaataa aaatggtcga aacctgcggg tctatccctg ctgccagata
      541 aattcgcgcc aaatccagag ttttttgttt cagaacctgc ggcttttgcg gcagcgtaat
      601 cgcgtgcaga ttgacgatgc aaaagaaact ctcgtattcg tgctgaagcg cgacccagtt
      661 tttcagcgcg ccgagataat tgccgatgtg cagctcgccg gtcggctgcg cgccgctaaa
      721 aatgcgtttt ttcataaact aaaagagaag gctcaaaacc agattattta acggcacgac
      781 aatcattcgg atgatgccga gctgcagcag gacgagcaaa atcaagaaac cgtaagtttc
      841 cagaatctcg aataaaggct cgaagctgcg cggcagaaaa gtctgcaaaa ccttgcttcc
      901 gtcaagcggc ggaaacggca gcagattgaa aatcgccaaa ccgacgttca tcaaaaccat
      961 cgtgtagaga aaattaaaaa gcaccgacac gagcccgacc tgaaaattgt ccggcgtgat
     1021 aacctgagaa gcgattaata ttttgataat cacggcggaa acggtggcga taatcaaatt
     1081 caccacgatt ccggcaatcg agaccatcac gttcgccgtg tccttattgc gccatttgag
     1141 cgggttgacg ggcgtcggct tgccccaggc gataagcgga aaattaaacg cgctcgtgta
     1201 gctgaggaca aagccgagaa tcgggagaac cagcgtgcct atcgggtcga tatgcgggat
     1261 tggattgacc gtcacgcgac cgagctggta agccgtatcg tcgccgaatt tataagagac
     1321 ccaggcgtgc gccgcctcgt gcagcgaaac ggcgaatatc cagactacca tgtaaataac
     1381 cagctgcgat acaaactgcg caatatcaaa atcattcata attccgctcc gaaaaaaaac
     1441 attggaatcc gcaaactaca accttggaaa gaagtattaa acatttcttt attaattgtt
     1501 tttacgatta aattgtgcca ttctttcaga acaaaataaa atcatcaacg gagatttaat
     1561 gttgaaacga atacttttcg gcggtgaaac gtccgcctcg ccgctgacga atttaggttt
     1621 gacgcttttg cggattttcg ccggaatcgg tttgatgacg cacggcatcg ggaaaattcc
     1681 gccgtcggaa aaatttatcg aaggcgtgac gggaatggga tttccggctc cgcaggcttt
     1741 cgcgtgggcg gctggactga gcgaatttgt cggcggcgcg tttttgattc tcggtttatt
     1801 tacgcgcccg gtcagctttt ttatcgcttg tacgatggcg acggctttaa tcggtcagca
     1861 tcgcaacgac ccgtttgccg tgcaggaaaa agcttttctg tatcttttca tcgcgctcgc
     1921 gtttcttttt atgggcgcta atttctggag cgtggacgga attctgcgcc gcaacagaag
     1981 tctctaaacc gaagcgaatc aagcataaac agggcgctga ttttttagta tcgattaaga
     2041 taagttaaga ttaattttat taatcctaaa taatactatt aatcgagcgt cctgttttct
     2101 cagtatgagc aaagataaaa attatatcgt gtgtatcgcc agcgagcaca aaggcaacga
     2161 ttttctggaa gaatgtcagg aagcgggctg gcacgtcacg ctggtgacga aagaaaaact
     2221 gctggacgag ccctgggctt ggacgagcct gaacggcgtg aaaaccgtgc ccgacgaagc
     2281 gacgaccgaa gattacattc gcgccgtcac caacgtcgcc ggaacgcagc ccgtgacgcg
     2341 catcgtcggt ttggacgaat tcgacgttct gaccgccgcc aaagcgcgcg agcatttgca
     2401 ggtcggcggc ggtatgaccg gctctttcgc cttgcgtttc cgcgacaagc tgcggatgcg
     2461 ccggctggca aagcacgtcg atattccgtg cccggaattt gccggcatca ttaatcgcgc
     2521 gcagattgac gaatatctgg aaagcgctgc cgcgccctgg attatcaagc cgcgcacgga
     2581 agtttccgct ttcggcatcc gcaaatgcga gacgaagcag caggtctggc aggttttaaa
     2641 cgagctcgac gcgcgcaata cgtggcgcga ccacccgtcg cagtttttga ttgagcgctt
     2701 tatcgaaggc agagtttttc acgtcgattc ggtcgtcgcc gacggcaaaa tcatcgcctc
     2761 cggcgtcagc gaatacggcg cgccgccgat gcgcgtatcg cacgaaggcg gcgtttttac
     2821 gaccgcgctt ctgccgtata aatccaaaga acgcaaagag ttggaaaaac tcaacaaaga
     2881 ccttttgaaa ggcttcgaat acgagcaggg cgtctcgcac gccgaatttt tgcagtctgc
     2941 cgaaaccggc gagttttatc tgctggaagt cgccgcccgc gtcggcggcg catatatcgc
     3001 gaacgttctg gaacaagcct cgggcttcaa tctctggcgc gaatgggcaa agctggaaac
     3061 ggcgacgaaa aaaaatccgt accagccgcc gaagctgcgc aaagattacg ccggaatcgc
     3121 gctggcttta tcgaaagaac aaacgcccga cacgtcgcat tacacggacg aggaaatcgt
     3181 ctaccgcgtc acgaaaccga aacacgtcgg cttgattttc ttctcgaaaa agcacaagcg
     3241 catcgaggag cttttaaaaa cctacacgga aaaaatcacg cgggattttc tggcggtcgc
     3301 gccggtcaaa gaaaggtacg acgattgaga aaataataaa ataatacata cggcggcggg
     3361 tttacagccc gccttttctt tttccgcccg attgcttatt ctaattattt tatgacggtc
     3421 gaaacagctt cacagacatc ttcgcctgcg attttagagc accatctgga gcgcgttttt
     3481 ccggctttgt tgaagtttga atcgacgccg gaaatgataa aatccgccgt ttatcaatcg
     3541 gtcaagccgg aagtcgagcg aattttaaat gccgtcgtgg aggaaaattt tgcgccggaa
     3601 acgtttgaga aaaccgacac ggcgcgcgct ctggcgtgga acatcgagcg cggaaatcgt
     3661 tttgagggaa tcgttgaagc gctgaaaaat cacccacagt tgaaagaccg tgacgttttg
     3721 cttttgacgg agctggatta cgggatggcg cgcagcggca atcgctttgt cgcgcgggaa
     3781 ctggcgcggc agttaaagtt aaattatgct ttcgcgccgg tttacatcgc tttgcagaag
     3841 ggaagcggcg tcgaatcgga agttgaaggc gagaacacga aatcgctgca cgggctggcg
     3901 atgttctcga aatatccttt gaaaaacgcc cacgccattc cgctgccgaa cgggaaggac
     3961 aaaatgctcg gcaaggaaaa aaggctcgga cacctgcgcg ctctgattgc cgacgtccgg
     4021 catccggcgg gcgtttttcg cgccgtgacg ctgcatctgg acgcgcattc ttcccgaaaa
     4081 catcgccagc gccagatgcg aatcgtgctc gaacatctgg aaactctgcc ggaactgccg
     4141 accgttatcg gcggcgattg gaacacctcg acctataatg cgcaaaacgc cacgcgcgcg
     4201 attctcggtt actggcggcg cgacatgatc ggcgcgaaaa gaaccgtgcg gaatcatttt
     4261 ccgcacccgg accgctattt tgaaaaaggg ttgttcggcg agctggaaaa acgcggctac
     4321 gattataaaa atctgaataa tcccggcgtc ggaaccctgc attacgacgt ggagagcatc
     4381 gagaaaaaca cgaatctgcg cgattgggtt cccgaatggt gttttccgtt tatcttctgg
     4441 gcggcgcgtc gcgtcggcgg gcgcgtctca ggacggctcg attggtttgc cggaaaggga
     4501 attaagttaa ccgggaagcc gcagactatc ggcaatttgt atgacgcgga aaacattccg
     4561 ctttccgacc acgacgcgat tgctctggac tttgcgccct cgattcgaca caaagacaca
     4621 aaagactcaa aggcgattca aatcgcttag tttatcgacg gacgactttt ccggctttgt
     4681 gttctttgcg cctttgcggt taaactttta gagaggaaaa taaaatggaa gtatcttttt
     4741 gtaaaacaac cagctttaat gccgataatt ttgaatacga aatcatcaaa gccgaaaacg
     4801 gctacgccga gatgattaaa tttccgattg acgagacgca gaacagcccg ggcgacgtcg
     4861 tcgtcataat agacggcgaa gacattcaat ttcacggcat tatcggcaaa atcgaggaag
     4921 gttacgcttt cgcatacgac ccgaaaggct cgaccttgcc cgccagcgtt cagtaaactt
     4981 gacagtttcg caatagattt ttatcatcta aattcgtttc tcgtggggat gtagctcagt
     5041 ttggcagagc gctgcgttcg caatgcagag gtcaggggtt cgacccccct catctccacc
     5101 aaatatttaa aggctttagc ttatttcggg ctaaagcctt ttatttataa ttccacattc
     5161 ggtattgttc agccggttat tgttccggca ttcactttca gtttgtttct taaaattaat
     5221 aatttcccgt tgagggaaca tttctgcatt gccgccgata actatcacag aagcacgttg
     5281 gcgcattaat tccggggacg tttcgccgag cactcaaatt acaaagccgt cgattttatt
     5341 taggagaaac tgatttttat gttgaatgtc gacctgttgt tagagcttgt tatttttatc
     5401 ttagccggtt ttttaatgat tgtggctggc gggacgtttt acgtctggtg gaacgggtat
     5461 cataaacccg acagtcatac acgccagcga cttcattaca agcgccacta ccgctcaaga
     5521 ctctgaagcc gcccgcttgg tttccgagat atattcaatc agctcaaaat gcctggctac
     5581 ttccagccaa tcgacaatcg gacgatcatt ttcgactttg acgagcgggc agccgaatgc
     5641 cgcgtcgtcg atataaatgt gcgcgtagac tttcggcgaa tccgtccagt atctttgcgc
     5701 cggattttcg ttgaccgccc aaaattcgat gcccgccgcc gccgcgtaat cgaccgcttc
     5761 ctgtaaatac tcgccgctcc gcatcgtgta aagaatcagc ttgtagcctt tcgcctgcat
     5821 cgcgcgcatc gtttccagag cgtacggaac aggctcgcct atttccgggt aacggtgcgc
     5881 gactatggtt ccgtcaaaat caatcgccac aatcggcgtt ttgggtttat tgctgcgctt
     5941 cattcttatc ctattagagc aaatttcggt ttgaatcgac aaagacggaa gcactctcta
     6001 aacgtgaaat tttgtattca tcgttcattg tttatcgttc gcctgccgca ggcagagcgc
     6061 tcgtgatttc ctcgattccg acgatgtttt taatttgccc ggacacttcg attgaaaaaa
     6121 gcgcgcgtag aaaaattaaa atccgacctt ttctgatttg cttcatatca atcagcccgc
     6181 attttttgat gccgcagtat aaaacgaatc cgaagtttca caataaaatg aaattaacat
     6241 aaaatttttt cagatactca aaaatcggat gaaacggaaa cgctgcaaag gaggctgtaa
     6301 atgctcagaa atttaacttt aatcgccttt tgtttgattg tgtcgacggg cgcggcgagc
     6361 gcgcaaaaaa aatcgaccga tacggaagta aagccaaaga ctttaaaaaa atccaaccag
     6421 cgtccggcgg gcaaaacgcc gaaagccgag ccgtttgaga aagcgaccgt cgaaataatg
     6481 tcggcgcagt gcgtcaagct tgagacggaa gcgggcgtca tcgaactgga gatgtttccc
     6541 gaaagcgcgc ccgaatcggt gcgaaatttt ttgaatctgg cggcaatcgg cgctttcgat
     6601 acgacgactt tcagccgcgt cgtgccgcgt tttgtcattc agggcggaaa cctgaccacg
     6661 cgcgaaaagc tgacgccgga actcgacaaa cggatgcgtc gcacgattcc ggacgagccg
     6721 aatcaaatca agcacgaacg cggaattctt tcgatggcgc gctcggacga gccgaacacc
     6781 gccagcacgc acttttttat tctcgtggac tcggctccga gtctggacgg aacttttgcc
     6841 gccttcgggc gcgtcacgca gggaatggaa gtcgtcgatg ccatcaacaa aatgccggtt
     6901 gaaaaagaca aaccgtcgaa acccgtccgt atcacgcgcg cgtcggtcgc cccttgcgcg
     6961 cctcaaacga ctactaaata acacgaaaac gagcgcgtct cttaaagaac acgctcgctg
     7021 accttcgctt ttccgccttt aaatatagaa aaatatcttc ggaatagata atatccgtca
     7081 ggtaatatta aggatagatt ttatctatcg gctatcaact gccggcaatc agct
//
LOCUS       DASWNE010000426         1786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_439822, whole genome shotgun sequence.
ACCESSION   DASWNE010000426 DASWNE010000000
VERSION     DASWNE010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439822"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VGB68_08575"
     CDS             <1..535
                     /locus_tag="VGB68_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049323.1"
                     /translation="AIIYQTKYFTFIIPVLHLPVQFSTLSIYFGTGTLFFLFRDEIPL
                     RFSWSLVALALWCLGFYMNMGVLFSYFCLPYITFWFVYEPKIKLHGIARLGDFSYGLY
                     IYSWTVQQTVIHFLGAQIPWLLMFVLSFLLTVPLAMFSWFAIEKKALRIKDRLFKKQE
                     AIQKPDVLTEQAEPLKS"
     gene            557..880
                     /locus_tag="VGB68_08580"
     CDS             557..880
                     /locus_tag="VGB68_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049324.1"
                     /translation="MKKILIAIFAAVFLLSACGLSGRNSSNQIIKGKSVSVKGKVTRI
                     ERGKDGYAAEIKTGDGKTYSAVISSVNLAGSGQYREVAIGEAIDVEGENVSSDGSTIR
                     VTVLR"
     regulatory      972..1080
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1072..>1786
                     /locus_tag="VGB68_08585"
     CDS             1072..>1786
                     /locus_tag="VGB68_08585"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF014067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEY0049325.1"
                     /translation="MSENILEKTSEQVKLPASRKIYVETNGNSINQNKHSLRVPFREI
                     ALSASKDFDGALEENPPVRVYDTSGVWTDPNEKCDVREGLPQLRREWILGRGDVEEYV
                     GREILPQDNGYLTKGAEQVARIKDRGKLEEFPGLKRAPLRAATGKCVTQMHYARRGII
                     TPEMEYVAIRENLGRQIAFETMQNGAGDDRSSLYHQHKGESFGASLPRFVTPEFVREE
                     VARGRAIIPSNINHPESEPM"
BASE COUNT          454 a          439 c          483 g          410 t
ORIGIN      
        1 ggcgattatt taccagacaa aatatttcac tttcatcatt cccgtgctgc atctgcccgt
       61 ccagttttct actctttcga tttatttcgg caccggcact ttattctttc tgttccgcga
      121 tgaaattccg cttcgtttct cgtggagcct cgtcgcgctc gcgctttggt gtttgggttt
      181 ttatatgaat atgggcgtcc ttttcagcta tttctgcctg ccctacataa ccttctggtt
      241 tgtctacgaa ccaaagataa aacttcacgg catcgcccgc ctcggcgact tttcctacgg
      301 gctttacatc tattcgtgga cagtccagca aaccgtcatt catttcctcg gcgcgcagat
      361 tccgtggctc ctgatgttcg ttttatcgtt tttattgact gtgccgctgg cgatgttctc
      421 gtggttcgcc attgaaaaaa aggctctgcg aatcaaagac cgattattca aaaagcagga
      481 agcgattcaa aaacccgacg ttttgacaga gcaagccgag ccgttaaaat cataagacga
      541 acagctgaaa atgaaaatga aaaaaatact gattgcaatt tttgccgccg tttttctgtt
      601 gagcgcctgc ggcttgagcg ggcggaattc gtctaatcaa ataattaaag gaaaaagcgt
      661 ttcggttaag ggaaaagtaa ctcgaatcga aagaggaaaa gacggctacg cggcggaaat
      721 caaaaccggc gacggaaaaa catattccgc cgtcatcagt tccgttaatc tggcgggctc
      781 ggggcaatac agagaggtcg caatcggcga ggcgattgac gtcgagggcg aaaacgtgtc
      841 gagcgacgga agcacgataa gagttacggt cttgagataa ataaattttt atttttggct
      901 ggcgaggcgc aaagacggcg gcgcaaaggc ggcggtcgcc tttccgcctt tgaattgata
      961 aacttctgtt gagcaatttt caggagagcc gcacgagcgg ctgagattcg cctgtcacgg
     1021 gcgttatcct ttgaacctgc tacggctaac accgacgaag ggagaaaaat aatgagtgaa
     1081 aatatactgg aaaaaaccag cgagcaggtg aaactgcctg cttcgcgcaa aatctacgtt
     1141 gaaaccaacg gcaattcaat caatcaaaac aaacacagcc tgcgcgtccc gtttcgggaa
     1201 atcgccctga gcgcgtcgaa ggattttgac ggcgctttgg aggaaaatcc gccggtgcgc
     1261 gtctatgaca cgagcggcgt ctggactgac ccgaacgaga aatgcgacgt gcgcgaaggc
     1321 ttgccgcaac tgaggcgcga gtggattctg gggcgcggcg acgtcgagga atatgtcggg
     1381 cgcgagattt tgccgcagga caacggttat ttgacgaagg gcgccgagca agtcgcgcgc
     1441 attaaagaca gaggaaagct cgaagaattt ccgggcttga aacgcgcgcc gcttcgcgcg
     1501 gcgacgggaa aatgcgtcac gcagatgcat tacgccaggc gcggcatcat cacgcccgaa
     1561 atggaatacg tggcgatacg cgaaaatctc gggcggcaaa tcgcgtttga gacgatgcag
     1621 aacggcgcgg gcgacgaccg ttcgtcgctt tatcaccagc acaaaggcga atctttcggc
     1681 gcgagcttgc cgagattcgt gacgccggaa tttgtccgcg aagaagtcgc gcgcggtcgg
     1741 gcgattattc cctccaatat caatcatccc gaatccgaac cgatgg
//
LOCUS       DASWNE010000427         2621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_398464, whole genome shotgun sequence.
ACCESSION   DASWNE010000427 DASWNE010000000
VERSION     DASWNE010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398464"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VGB68_08590"
     CDS             <1..660
                     /locus_tag="VGB68_08590"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0049326.1"
                     /translation="AVFRPSEGMWYIQRSSDFGFVASQFGNSTDIPVQGDYNGDGRTD
                     LAVFRPSTGVWYIARPTGVPSQNFDSIQFGQSGDRPVQGDYDGDAKTDVAVYRNGNWY
                     VLRSSDGAFVAQQFGLGSDVPVVGDFDGDGRVDFTVYRDNTWYILQSLTGSFRAVQFG
                     TTGDIPMAADFDGDGTADITVFRPSNGFWYVLRSSDGVFVVQQWGQNNDVPVPAAYQP
                     R"
     gene            983..1318
                     /locus_tag="VGB68_08595"
     CDS             983..1318
                     /locus_tag="VGB68_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049327.1"
                     /translation="MFEFKGDLPNYSVNYVEPPIWLGESGEKVKISGKAFVEVSFYAV
                     PIPENGYFPDKLLEFPKEKLNLPVVNEIKMSDWWEGVLALAVGLKAKKLYRVEELSNP
                     ARLVVDFKQ"
     gene            1331..2617
                     /locus_tag="VGB68_08600"
     CDS             1331..2617
                     /locus_tag="VGB68_08600"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY0049328.1"
                     /translation="MQTEFTFNRGAVRPMQCLSGGWQLLRGAYGNFLGVVIVGLLLMI
                     AGSCIPLAPLNAPVLCGIYLCLLARIYNQPFNTSTLFKGFQYFAQSFIASLFVSVPMF
                     VLGLIFQFGMGGFQLALGNLKIDEKTPPEQVLPLVFGFFGYIFGGILVIMLVGILLNA
                     LMLFVYPLIVERNLSGLEAVKLGFRAVLGNFFGVFILAVLEILLILAGMMLFYIGAIF
                     VAPLIFAARIVAYRQVFPAPVPQADAQQLNTGAPQQMWTPQMITSKAGWYLTASAVLI
                     LGLAAAGIAGLSIWSYTAISEAVKKAQQQRRDDRNTSYYPTPSPAQYNSNRNSPNNNS
                     QTIGGDVLNDKAVELPSPVYPPAAKALKASGNVVVQVTVNEKGEVTSAGAVSGHPLLR
                     SAAEQAARKAKFKPNPQNGKASETKGILNYNFTAPG"
BASE COUNT          593 a          676 c          718 g          634 t
ORIGIN      
        1 gctgtcttcc gtccgtctga aggcatgtgg tatatccagc gcagttccga cttcggtttt
       61 gtcgcatcgc agttcggcaa ctcgaccgat attccggttc agggcgacta caatggcgac
      121 ggcagaaccg atttggctgt cttccgcccg tcaaccggag tttggtatat cgccagaccg
      181 accggcgttc cgtcgcagaa ctttgactcg attcagttcg gacaaagcgg cgaccgtccg
      241 gttcagggcg actatgacgg cgacgcgaag actgacgtag cggtttaccg caacggcaac
      301 tggtatgtcc ttcgcagttc ggacggcgcg ttcgtcgcgc agcagttcgg cttgggcagc
      361 gacgttccgg tggtcggcga ctttgacggc gacggcagag tcgactttac ggtctatcgc
      421 gacaacacct ggtacattct ccagagcctg acgggaagct tccgcgccgt gcagttcggc
      481 acgaccggag acattccgat ggcagccgac tttgacggcg acgggacagc ggacatcact
      541 gtcttccgtc cgtcgaatgg cttctggtat gttctgcgca gctcggatgg cgtgtttgtc
      601 gttcagcaat ggggacagaa taacgacgtg ccggttcctg cggcgtatca accgcgttaa
      661 taacggctgc gtccgctatt aacacttaac ctgagaggcg ggaaaatttt cccgcctctt
      721 ttttctttgg caaaaagcgc atcgattgac agtcaataaa taactgtttt accaaagaaa
      781 tctttatgaa aatttatcga aaattattaa tcgtaagctt tttactgtct atcgttttac
      841 gacgcccgcg acggcgcagt tttatgagaa taaaggcagc gtcaaggcgt ggacgaaaaa
      901 gaccgtctcg cgcgggaaaa agtatgaaaa caccgagttt gtcaaagtca aagtcggaaa
      961 gcatcccggc tacgaccgcg tcgtgttcga gttcaaaggc gatttgccga actactcggt
     1021 taattacgtc gagccgccga tttggctggg cgaaagcggc gagaaggtga aaatcagcgg
     1081 caaagctttc gtcgaagttt cgttttacgc cgttcccata ccggaaaacg gctattttcc
     1141 ggacaagctt ctggagtttc ccaaagaaaa attaaatttg ccggtcgtca acgaaataaa
     1201 aatgtcggac tggtgggaag gcgttctggc tttagccgtc gggcttaaag cgaaaaaact
     1261 ttaccgcgtc gaagagcttt ccaatccggc gcgtctggtg gtcgacttca aacaataaaa
     1321 ggaaaaacca atgcaaacgg aatttacatt taatcgcggc gcggtgcgtc ccatgcaatg
     1381 tctgagcggc ggctggcagc tgctcagagg cgcttacgga aattttctgg gcgtggtaat
     1441 cgtcggtctg ctgttgatga ttgccggcag ttgtattccg ctcgcgccgc tcaacgcgcc
     1501 cgtattatgc ggcatttatc tgtgcctgct ggcgcgaatt tacaatcagc cgtttaatac
     1561 gagcacgctt tttaaaggat tccagtattt cgcgcagtcc tttatcgcat cgcttttcgt
     1621 ctccgtgccg atgtttgttc tcgggcttat ttttcaattc gggatgggcg gttttcagct
     1681 cgctctgggc aatctgaaga tagacgaaaa aacgccgccc gaacaggttt tgccgttggt
     1741 gttcgggttt ttcggctata tattcggcgg aattctggtc ataatgctgg tcgggatact
     1801 gctgaatgcc ttgatgcttt tcgtttaccc gttgattgtc gagcgcaatc tgagcgggct
     1861 ggaagccgtc aagctcggat ttcgagccgt tctcggaaat tttttcggcg tgttcatcct
     1921 ggcagtttta gaaattttat tgattctggc tggcatgatg cttttttata tcggcgcgat
     1981 tttcgtcgcg ccgttgatat ttgcggcgcg catcgtcgct tatcgacaag tttttcccgc
     2041 gcctgtcccg caggctgatg cgcagcagct aaatacgggc gcgccccagc aaatgtggac
     2101 gccgcagatg ataacttcga aagcgggctg gtatctgacg gcgagcgccg tattaattct
     2161 cgggctcgcc gccgccggca tcgccggtct gagcatctgg agctacaccg ccataagcga
     2221 agccgtcaaa aaagctcagc agcagcggcg cgacgacaga aacaccagtt actatccgac
     2281 gccttctccg gcgcagtata actcgaacag aaattcgccg aacaacaatt ctcagacaat
     2341 cggcggcgac gtcttgaacg acaaagccgt cgagctgccg tcgcccgttt atccgcccgc
     2401 cgccaaagcc ctgaaagctt ccggaaatgt cgtcgtgcag gtcacggtta atgaaaaagg
     2461 cgaggtgacg agcgccggcg cggtttccgg tcatccgctt ttaagaagcg ctgccgaaca
     2521 agccgcgcga aaagcgaaat ttaaaccgaa tccgcagaac ggcaaagcgt cggaaacgaa
     2581 aggaattcta aattacaatt tcaccgcgcc gggctaaagc g
//
LOCUS       DASWNE010000428         5816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_226030, whole genome shotgun sequence.
ACCESSION   DASWNE010000428 DASWNE010000000
VERSION     DASWNE010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5816
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226030"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(397..1842)
                     /gene="dnaA"
                     /locus_tag="VGB68_08605"
     CDS             complement(397..1842)
                     /gene="dnaA"
                     /locus_tag="VGB68_08605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEY0049329.1"
                     /translation="MNTSTEQNTNVWNSILQSVEKRLNRQIFEAWFRPIQFEGFDAQE
                     KVLRLRASQVNKDWVSTYYSDVIKQTLHELNLASYRLDWEVEETKMPEENFLEDDADF
                     FFEKQTTQNISNNFNSLEKTRPEALPKQNTTNFVDIEPIENSLNQKYSFGTFVVGSCN
                     QFAHAASLGAADAPGKTYNPLYIYGGVGLGKTHLMHAIGHSIKESNRHLRVAYISAEK
                     FMNELINAIRYDKTQTFRDKYRSMDVLLMDDVQFMAGKERTQEEFFHTFNALHNDQKQ
                     IVITSDCPPREIPTLEERLHSRFEWGLIADIEPPDLETKVAILKRKADLDGVQLPDDV
                     AFFIAGKVKSNIRELEGSLVRLVAISSLRGMPISKMLAQDAIRNLVDHESPEGLSMDR
                     ISKAVAAHYKLTVEELKSKNNSRQIAVPRQVAMYLCKRLTKHSFPEIGRDFGGKHHTT
                     VMHSVEKIDSLIKEDRNFHRVVSDLIDNLCS"
     gene            2265..2693
                     /locus_tag="VGB68_08610"
     CDS             2265..2693
                     /locus_tag="VGB68_08610"
                     /inference="COORDINATES: protein motif:HMM:NF019537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease A inhibitor family protein"
                     /protein_id="HEY0049330.1"
                     /translation="MPKKKKSIKSEPFEKPAEQIRKASEGLYYISETDAEISSFTGTK
                     ADAVTRENLLSQTGNNRDAPVEERDFTDFFTRLTKIQDWFGDEEKRTAAKFSRLKELL
                     ERNLKDLKVYKVGRIEIDIYVVGLDAQGNLAGIKTKAVET"
     gene            complement(2861..3556)
                     /locus_tag="VGB68_08615"
     CDS             complement(2861..3556)
                     /locus_tag="VGB68_08615"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HEY0049331.1"
                     /translation="MRKVKIIVFIVALIAGLTFAKMSAFSFGFNMPGINVFSKVKGSG
                     NIQIEKRDASAFTGVKAGGAITVDVTAQRDFSVEVEADDNLLKHIKTEVDGNTLKIYT
                     EGNISTRNPIRVRIAMPQIESFDVSGASTGSLTNIKNETLSLDASGASKIKIDGEARE
                     LIVDLSGASRLDAENLRAEDVTVDASGASSASVVALNDLNADASGASTIRYTGEPKNV
                     KKNSSGASSVRQK"
     gene            complement(3633..5240)
                     /locus_tag="VGB68_08620"
     CDS             complement(3633..5240)
                     /locus_tag="VGB68_08620"
                     /inference="COORDINATES: protein motif:HMM:NF016196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HEY0049332.1"
                     /translation="MDALQEIFRNLWAQIALLLIFATVHGYLGAWLAVRMLFRPRNPV
                     KIFGLTIFPQGMIPRHRERLAMAIGKAVGEELVSQETVLDELFEKDFLRKKIQTVVDS
                     YTGDILSQNYPSLIDTLPSNLREPVLDAISSLQLKIGDYVTGVLKSEETLETIHNFVE
                     RRVDEVLSRRVSETIDEENFYKILNFLETRVRSIVKEPVFEQKIRDFINKQVDVLMNT
                     NISLGEMVTEDAVGLLKEKAGEQINPIVHQLAELATAERTRNQIGSLIKREVHAYYEN
                     LPFFKKIFVSRENLLKEVDDLVNENLPKRIEETLRGDFFAQEAQNFLDKAIGNAMSRP
                     LPEVIGKIAPEQLERLKSQIIKGVLSVLQGEEMQNSISAYLTDTLHKIRPHSIDAILQ
                     TIHPQAEDKLKNMLSKGLLGILNRDETSNIINSVLSRQIERLLSAPIGRLSDHISEEQ
                     VRSAGKSLTETIIAAAREKLPQVIQEFNLGNVVREKINNYPVEKLENLVLSVAKEHLR
                     TIELFGALFGLLIGIMQAIQFYFYAAK"
     gene            complement(5240..5623)
                     /locus_tag="VGB68_08625"
     CDS             complement(5240..5623)
                     /locus_tag="VGB68_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049333.1"
                     /translation="MSFTLMDLSSENFEFRANVWNWKPTLEIIKSFDIMSDGKLRQME
                     YNATGVSISKEEAHEIGEKIRDTILPKLEPNKRIFMDLSVTDAPDDGTLYKDEDEQWK
                     NYSASYEWLKDFSDFCLNSKGFQVY"
BASE COUNT         1510 a         1322 c         1339 g         1645 t
ORIGIN      
        1 aactgctaat aaacagatgt ttctttttcg ttaaaaccta aagtttaact tttataaatc
       61 acacgccttt tactatttct aatacattaa aacttatata tatttcttag tagtagtagt
      121 agtagctgtg gaaaagtgga aaaccggctt tactgttgaa ataattgatt ttatctttcc
      181 acaggcggtg tggaaaactc tgtggaaaac tcgtcgcctg tggaaaaaaa tgacgacacg
      241 acgacttttc caccgatttc cacagggcag attttcaaca gcctgtggaa atgtggaaaa
      301 ctttgtttaa gtgttgtcgc taaactatat aaacagtttc gctaaagctt gtccttgtgg
      361 aaataattcc actctttccg gttaaatcag ttttaattaa ctgcaaagat tatctataag
      421 gtcgctgacg accctgtgga aatttctatc ttccttaatt aacgagtcaa tcttctcgac
      481 cgagtgcatt acggtcgtgt gatgcttgcc gccgaagtcg cgaccgattt cggggaagct
      541 gtgtttcgtc agacgcttgc acagatacat cgccacctgt cgcggcacgg caatctggcg
      601 cgaattgttt tttgatttca actcttcgac ggttaatttg taatgcgccg cgacggcttt
      661 cgagattctg tccatcgaca aaccttcggg cgattcgtgg tcaaccagat tgcgaatcgc
      721 atcctgcgcg agcatcttgg aaatcggcat tccgcgcaag gaagaaatag cgacgagccg
      781 caccagcgag ccttccagct cgcgaatatt gcttttgact tttccggcga taaaaaaagc
      841 cacgtcgtcc ggcaattgaa cgccgtccaa atccgctttg cgtttcagaa tggcgacttt
      901 cgtttccaaa tccggcggct cgatgtcggc aatcaagccc cattcaaagc gcgaatgcag
      961 gcgttcttcc aaagttggga tttcgcgcgg cgggcagtcc gaggtgatga cgatttgttt
     1021 ctggtcgtta tgcagcgcgt taaaggtgtg gaaaaattct tcctgcgtgc gttctttgcc
     1081 agccataaac tgcacgtcgt ccattaacaa aacatccatc gaacgatatt tatcgcggaa
     1141 agtctgcgtt ttgtcgtaac gaatcgcgtt aatcaactcg ttcataaatt tttccgccga
     1201 gatataggcg actcgcaaat ggcggttact ttcttttatc gaatgaccga tggcgtgcat
     1261 caggtgagtt ttccccagac cgacgccgcc gtatatatat aaaggattat aggttttgcc
     1321 cggagcatcc gccgcgccga ggctggcggc gtgtgcaaac tgattgcagg agccgacgac
     1381 aaaagttccg aacgaatatt tctggttcag agaattttca atcggctcga tgtcgacgaa
     1441 attcgtcgtg ttctgtttcg gtaaagcctc gggtcgagtc ttttccaaag agttgaaatt
     1501 atttgaaatg ttttgtgtcg tctgtttttc aaaaaagaaa tccgcgtcgt cttcgagaaa
     1561 attttcttcg ggcattttcg tttcctcgac ttcccagtcg aggcgatagc tcgccagatt
     1621 cagctcgtgc agagtctgtt ttatcacgtc cgaataatac gtgctgaccc aatccttatt
     1681 gacctggctc gcacgcaggc gcaaaacttt ttcctgcgcg tcaaatcctt caaactggat
     1741 aggacgaaac caggcttcaa aaatttgccg gttcagacgt ttttctaccg attgaagaat
     1801 gctgttccaa acgtttgtat tttgttctgt cgaagtgttc atatcttttc tccgcgacga
     1861 gcgaaaaatt tgttaaatta gctgcgaaac ttttaagcag tttgccggaa aaacttttct
     1921 gcaaaactaa ttcgggaaac tcttaataaa cctttcaaaa atatacgatt gaacgttgtt
     1981 aaaggcatat ttcagtaagt tttccacagg gttttccaca ggcttgtgga aaggtgctta
     2041 aaagctaatg taaaacctta acttatttgt tttcaatcac ttacaagagt taaatcattg
     2101 tgaaacgggt gaaagactaa cacaaaaaaa gggcaaatgc aagaagaaat ccacaggttt
     2161 tttgaaaaaa tatttttggc tgaaaaagcc tttttttaca aaggatttgg cagattttcg
     2221 gtttatgtta gacttgtttc gctggcagtt tacagctaaa aattatgccg aaaaaaaaga
     2281 aatcgataaa gtccgagccg tttgaaaaac cagccgaaca gattcggaaa gcctcggaag
     2341 gtctgtatta catcagcgag acggacgcgg aaatttcctc ctttaccggg acaaaagccg
     2401 acgccgtgac cagagaaaac cttttgagcc agaccggaaa caaccgggac gcgccggtcg
     2461 aagaaagaga ttttacggat ttttttacgc gtttgacaaa aattcaggat tggttcggcg
     2521 acgaagaaaa gcggacggca gcgaaatttt cccgacttaa agaattactt gaaaggaatc
     2581 tcaaagattt aaaagtctac aaagtcgggc gcatcgagat tgatatttat gtcgtcgggc
     2641 tggatgctca gggaaatctg gcgggaatta agacgaaagc cgtcgaaacg taaaaacatc
     2701 aaaaggcgcg cgggcgatga agcaagcgcg gctcgcagcc tgaaagacac gctcatcgac
     2761 cgtctgcctt ttcgcctttg ttcttcattt caatactaaa ttaaaaagca ggattgattt
     2821 tcaatcctgc ttcagctcgc gaaaaaagat ttccggaaaa ctatttttgc ctgaccgagc
     2881 ttgcacccga agaattcttt ttcacgtttt tcggctcgcc cgtatagcgg atggtgcttg
     2941 cgccggaagc atcggcgtta aggtcattca aagccaccac gctcgcgctg ctcgcgccgc
     3001 tcgcgtcaac cgtcacgtct tccgctctca gattttccgc gtcgagacgg ctcgcgccgc
     3061 tcagatcgac gattaattct ctggcttcgc cgtctatttt gattttcgaa gcgccgctgg
     3121 cgtcgagcga cagagtttcg tttttaatgt tggttaagct gccggtcgaa gcgccggaga
     3181 cgtcgaagga ttcgatttgc ggcatcgcga tgcggacgcg aatcggattt ctggtcgaaa
     3241 tattgccttc cgtgtaaatt tttaaagtat tgccgtcgac ttcggttttg atgtgcttca
     3301 gcagattgtc gtcggcttcg acctcgacgc tgaaatctct ctgcgccgtg acatcgacgg
     3361 ttatcgcgcc gccggctttc acgcccgtga aagcggaagc gtctcttttt tcgatttgga
     3421 tattgcccga gcctttaact tttgaaaaga cattgatgcc cggcatatta aagccgaaag
     3481 aaaaagcgga catcttggca aacgtcaatc cggcgattaa tgcgactatg aaaacgatga
     3541 tttttacttt tctcatatta ttctccagga aaatgattga atgacgaatc gtctctctga
     3601 aaaacgacct ttttcggaaa aatgttcaaa gtttatttcg ccgcgtagaa ataaaattga
     3661 atcgcctgca ttattccgat tagcaagccg aacagcgcgc cgaacaattc gatggttcgc
     3721 aagtgttctt tcgccactga aagcaccaga ttttcgagct tttcgaccgg ataattgtta
     3781 attttttcgc ggacgacgtt gccgaggttg aattcctgta taacctgcgg cagtttttcg
     3841 cgagcggcgg cgataatcgt ttcggtcaga gattttccgg cgctgcgcac ttgttcttcg
     3901 gaaatatggt cggaaagtct gccgatcggc gcggaaagca ggcgctcgat ttgccgcgac
     3961 aaaaccgaat tgatgatgtt cgacgtttca tcgcggttta aaatgccgag caagcctttg
     4021 gaaagcatat ttttaagctt gtcttcggct tgcgggtgaa tcgtctgcaa aatcgcgtcg
     4081 atgctgtgcg gacggatttt gtgcagcgtg tcggtcagat aagcggagat tgaattctgc
     4141 atctcctcgc cctgcaaaac cgagagaacg cctttaataa tctgtgattt cagtcgttcg
     4201 agctgttcgg gggcgatttt gccgataact tccggaagcg ggcgcgacat tgcgttgccg
     4261 attgctttat cgagaaagtt ttgcgcttcc tgcgcgaaaa aatcgccgcg caaagtttct
     4321 tcgatgcgtt tcggcaaatt ttcattgacc aaatcatcga cttctttcaa aagattttcg
     4381 cgcgagacga aaatcttttt gaaaaacggc agattttcgt aataggcgtg aacttcgcgt
     4441 tttattaacg aaccgatttg attgcgcgtc cgctcggctg tcgccagctc ggctagctgg
     4501 tgaacgatcg gattaatctg ctcgcccgct ttttctttta aaagtccgac ggcgtcttcg
     4561 gtgaccatct cgcccagaga aatattcgta ttcatcagga cgtcaacctg tttgttgata
     4621 aaatcgcgga ttttctgctc gaaaaccggc tctttgacga ttgagcggac gcgcgtttcc
     4681 agaaagttca gaattttata gaagttttcc tcgtcaatcg tttccgaaac tcttctggat
     4741 aaaacttcgt cgacgcggcg ctcgacaaaa ttatgaatcg tttcgagcgt ttcctcgctt
     4801 ttcaaaacgc cggtaacgta atcgccgatt ttcaattgca gcgaagaaat cgcgtccagc
     4861 acaggctcgc gcagatttga aggaagcgtg tcaatcagcg acgggtaatt ctgcgaaaga
     4921 atgtcgccgg tgtatgaatc gacgaccgtc tgaatttttt tgcgcagaaa atccttctcg
     4981 aaaagctcgt ccagaaccgt ttcctgcgaa accagttctt cgccgacggc ttttccgata
     5041 gccatcgcca ggcgttcgcg gtgacgcgga atcattccct gcggaaaaat cgtcagaccg
     5101 aaaattttta cagggttgcg cgggcgaaaa agcatccgca cggcgagcca cgcgccgaga
     5161 tagccgtgaa cggtggcaaa aataagcagc aatgcaatct gagcccagag atttctgaaa
     5221 atttcctgta aagcgtccat tagtaaacct gaaacccttt tgaattaaga caaaaatctg
     5281 aaaaatcctt gagccattcg taagacgcgc tgtaattttt ccattgttcg tcttcgtctt
     5341 tatagagcgt tccgtcatca ggcgcgtccg ttacgctcaa gtccataaaa attcttttgt
     5401 tcggctctaa tttcggcaga atcgtgtcgc gaatcttctc gccgatttcg tgcgcttctt
     5461 cctttgaaat actgacgccg gtggcgttat attccatctg ccgcagttta ccgtcgctca
     5521 taatgtcaaa gcttttgata atttccagcg tcggtttcca attccatacg tttgcccgaa
     5581 actcaaagtt ttcggaactt aaatccatta aggtaaaact catttttcca attcccccgt
     5641 tatagaaaat ttaatttcta aaaaaatgct aaacgcgaaa ggcaagtcta gtcaatgaag
     5701 ttttgcttag atggtttgac gagtataatt ttttgttaag taaatttatg aacacgaaag
     5761 aatatcttcg ccgaatcgga attgacgaaa cgagcttttc ggcagattcg gagagt
//
LOCUS       DASWNE010000429         5615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_50072, whole genome shotgun sequence.
ACCESSION   DASWNE010000429 DASWNE010000000
VERSION     DASWNE010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50072"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..4274
                     /locus_tag="VGB68_08630"
     CDS             9..4274
                     /locus_tag="VGB68_08630"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0049334.1"
                     /translation="MERKQAQQIYEKAKQEGKVAALLEQQRPNVFTQAVANITPGAEI
                     KVIISYVETLRYADDAYEFRFPMTIAERYIPSTVEPEDAAKISPQSKTRPGHTIALEV
                     EIEAGVSIENIASNTHEIQAQQYSASRFSIKLKNENEIPNRDFVLKYKTAGSGIEDAV
                     LAHKDKNGGFFTLILQPPDKILPQDTTPKEIVFVLDTSGSMSGFPIDKAREAMKLTLD
                     NLNPDDTFNLITFAGETQILFDAPVPATKENLARAQKLLDGVQSSGGTEMMKAIKAAL
                     EPSDSQQHVRIVCFMTDGVVGNEAEIIAEVRKYANARVFAFGIGDSVNHYLLDEISRE
                     GRGEVEYVSRTDDGSAAARRFYERIRNPLLTDVALEFQGIQTTEVYPREIPDLFDAKP
                     VTVVGRYAQGGRGKVVLRGKMQGQLFSREIEVEFPGQNSRNDALATLWARRKVADLTR
                     QNLSNRQKSSETKTDLEQAITNLGLEFRLLTPFTSFVAFDEQIVTDGAQTKRVEVPVA
                     SAENLPVKAEYSTQYGQATVGTVNASVSIVSTGATIDATESKIQTTITNREVISYLDR
                     GKGLQSAFSIGAGITQINENQNRFQPGWLSAAGQRPTSNYFTVDDLSANTGAAAGETS
                     ISGYIGALPNLTAAGGTNSLNALEATSEVTIRTLPSAKEGRSAGATVDFISKRGINSY
                     RGSLFETVGNEAFNANDFFANSRGLERAAARLNQFGGTIGGFLLKDKAWFFGSYEGLR
                     LRQPAFSISEVPALVSRQNAAANVRPLFDAFPLPNGQPTSNGFAEFAAVFTNPAAHDV
                     FAFDLDTRPVEELQINANYNFVDSKAALRGDRDFSLNTLRNFETRTNSVSISTAYTLS
                     TSAIAKARANFSRNRLRQDFTLDSFGGASVSPSIFAASPFSFLKYDLAGKNSALASGK
                     PTETTINQFQANGSLEWIINTHVFSIGADFRRLAFRIGAQPSERSVLFSGVNLSGTAL
                     RINQLTRTQPQNSALGNFSLYAQDAWRLTSNFNVNLGLRWDMDFAPKLAAENINFRNA
                     SPQMRDNFKNFAPRAGFAFDPLNNGKIALRGSVGLFYDFGNAAASEVFADSFPFAAGS
                     FARSVDWTASPESAFKPLLAFDAKLQTPRTWKILAEYQHEIFGNNLLTINYMGAFGRR
                     LFLTRTLFNADPNYNFVRLTNNDAESDFHAANVLYEKRFAQNFSVSTRYTLAKSTDNF
                     SPGNLRQNVFAGEDLSDERGASDFDVRHQLSVYGVYDIQTPFRSSAINFLTRDWTIFG
                     FANARTGFPLNVTYARVNDFGVELLRPDLISNAPLYTGAGGGKQINPAAFSIPAAGRQ
                     GTLGRNALRGFPFFQMDLGLERRFRLGGESNLRLTMEVFNVLNNTNFADMSGNLGTLA
                     ANESFLPNSYFGKSASTFGSQNFTPFYLYGGARTIQFSAKFVF"
     gene            complement(4489..5523)
                     /locus_tag="VGB68_08635"
     CDS             complement(4489..5523)
                     /locus_tag="VGB68_08635"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0049335.1"
                     /translation="MAKKTKALAKTEKKILITGGTGFLGTHIVRRFLADGEKNLRVMA
                     SSVPEWMKDAGVEAAEGSVTNRETVAAAVRNVSAIYHLAGKVSRDNEDAAAMNKIHVQ
                     GTRILCEAAKEASVKAFILASSSGTIAVSETEEILDETYPPPIEIISRWAYYASKYYQ
                     ERTALENFDGKGLKLVILNPSLLLGTEDERLSSTKPVLDFLARKIPYTPSGGLSFVDA
                     RDAAEAFVQALEKGSHREKYLLGAENMSFKEFFGRLERLSGVSAPMLRVPKKLAMTGA
                     SLIESVFKNWNKTSPVQPKEVEQAEYFWYFDSTKAEETLNFQPRDAQETLQDTISYLR
                     QNFLGDGVFK"
BASE COUNT         1334 a         1521 c         1437 g         1323 t
ORIGIN      
        1 gcaggataat ggagcgcaaa caggcgcagc aaatctacga aaaagcgaaa caggaaggaa
       61 aagtcgccgc tctgctcgaa cagcagcgcc cgaacgtctt cacgcaagcc gtcgccaata
      121 tcacgcccgg cgccgagata aaagtcatta tcagctatgt cgaaacgctg cgatacgccg
      181 acgacgctta cgaattccgg tttccgatga ccatcgccga gcgttacatt ccgtctacgg
      241 tcgagccgga agacgccgct aaaatctcgc cccaatcaaa gacgcgtccc ggtcatacga
      301 ttgcgctgga agtcgaaatc gaagccggag tttcaatcga aaatatcgct tcgaacacgc
      361 acgaaatcca ggcgcagcaa tattccgcga gccggttttc gataaagctc aaaaacgaaa
      421 atgaaatccc gaaccgcgat ttcgttttaa aatacaaaac cgccggcagc ggcatcgaag
      481 acgccgttct ggcgcacaaa gacaaaaacg gcggattttt cacattgatt ctgcagccgc
      541 ccgataaaat tctgccgcag gatacgacgc cgaaagaaat cgttttcgtg ctggacactt
      601 cgggctcaat gtccggcttc ccgattgaca aagctagaga agcgatgaag ctgacgctcg
      661 acaacctgaa tcccgacgat acttttaatc tcatcacttt tgccggcgaa acgcagattt
      721 tattcgacgc gcccgttccg gcgacgaaag aaaatctcgc tcgcgcgcaa aaactgctcg
      781 acggcgtcca gtccagcggc gggacggaaa tgatgaaggc gattaaagcc gcgcttgagc
      841 cttcggattc gcagcagcac gtgcgaatcg tttgttttat gaccgacggc gtcgtcggca
      901 acgaagcgga aataatcgcg gaagtgcgca aatacgccaa cgcgcgagtc tttgctttcg
      961 gcatcggcga ttcggttaat cattacctgc tcgacgaaat ttcgcgcgaa ggtcgcggcg
     1021 aagtcgaata tgtttcgcga acggacgacg gttcagccgc cgcccgaaga ttttatgaac
     1081 gcatcagaaa tcctctgctg accgatgtcg cgctcgaatt tcaaggcatt cagacgacgg
     1141 aagtttaccc gcgggaaatt cccgatttat ttgatgcgaa acccgtcacg gtagtcggca
     1201 gatacgcgca gggcgggcgc ggaaaagtcg tcctgcgcgg aaaaatgcag gggcaattgt
     1261 tttccagaga aatcgaggtc gaatttcccg ggcaaaattc ccgcaacgac gcgctggcga
     1321 cgctctgggc gcggcgaaaa gttgccgatt tgacgcggca aaatttgtcg aaccggcaaa
     1381 agagcagcga gacgaaaacc gatttggaac aggcaatcac gaatctcggg ctcgaatttc
     1441 gcctcctcac gccgttcacg tctttcgtcg cctttgacga gcaaatcgtc acggacggcg
     1501 cgcagacaaa acgagtcgag gttcccgtcg cgagcgccga aaatttaccg gtaaaagcgg
     1561 aatattcgac gcaatacggt caggcgaccg tcggcaccgt caacgcatcg gtttcgatag
     1621 tcagcaccgg cgcgacgatt gatgcgacgg aatccaaaat acagacgacc atcaccaacc
     1681 gcgaagtgat ttcgtatctc gaccgcggca aaggtctgca atcggctttt tcaatcggag
     1741 ccggcatcac gcaaatcaat gaaaaccaga accgttttca gccgggctgg ctttccgccg
     1801 ccggacagcg cccgacttcc aattatttta cggttgacga tttgagcgcg aacacgggcg
     1861 cggcggctgg agagacttcc atctccggct acatcggcgc gttgccgaat ttgacggctg
     1921 cgggcggaac caattctttg aatgcgctgg aggcgacaag tgaagttacc atccgtacgc
     1981 tgccgtcggc aaaagaaggg cggagcgccg gagcgacggt tgattttatc tccaagcgcg
     2041 gaataaattc ctatcgcggc tctttgtttg aaactgtcgg caacgaagcc tttaacgcca
     2101 acgacttttt cgctaattcg cgcgggctcg aacgcgccgc cgcgcgcctg aatcaattcg
     2161 gcggaacgat cggcggtttt ctgctgaaag ataaagcctg gtttttcggc agttacgaag
     2221 gcttgcgtct gcgccagccg gctttttcca tttcggaagt tccggctctc gtttcgcgtc
     2281 aaaacgccgc ggcaaacgta cgaccgcttt tcgacgcttt tccgcttccg aacgggcagc
     2341 cgacctcaaa cggcttcgcc gagtttgccg ccgtttttac caatccggcg gcgcacgacg
     2401 ttttcgcttt tgacctggat acgcgcccgg ttgaagaact gcaaatcaac gcgaattata
     2461 atttcgtcga ttcgaaagcc gctctgcgcg gcgaccggga tttttcgctg aacacgcttc
     2521 gaaattttga aacgcgcaca aactccgttt ccatttctac cgcctatact ttgtcaacca
     2581 gcgccatcgc caaggctcgc gcaaatttca gccggaaccg cttaagacag gatttcacgc
     2641 tcgacagttt cggcggcgca agcgtatcgc cgtcaatctt tgcggcttcg cctttcagtt
     2701 tcctgaaata cgatttagcg ggaaaaaatt ccgcgctggc gagcggcaaa ccgaccgaga
     2761 cgacaatcaa tcaatttcag gcgaacggct cgctcgagtg gataataaac actcatgttt
     2821 tttcaatagg cgcggatttt cgccgactgg catttcgcat cggcgcgcag ccgagcgagc
     2881 ggagcgtttt gttctccggc gtaaatttga gcggaacggc tttaagaatc aaccagttaa
     2941 ccagaacgca gccgcaaaat tccgcgctcg gaaatttttc tctctacgcg caggacgcgt
     3001 ggcggttaac gtcgaatttt aacgtcaatc tcggcttgcg ctgggatatg gattttgcgc
     3061 cgaaacttgc ggcggaaaat ataaatttcc gaaacgcctc gccgcagatg cgcgataatt
     3121 tcaaaaattt cgcgccgcgc gccggatttg ccttcgaccc gctcaataac ggtaaaatcg
     3181 cgctgcgcgg ctcggtcggt ttgttttatg atttcgggaa cgcggcggcg agcgaagttt
     3241 tcgccgattc gtttccgttc gccgccggaa gctttgcgcg cagcgtcgat tggacggctt
     3301 cgccggaaag cgctttcaag cctttgctcg cgtttgacgc aaaattgcag acgccgcgca
     3361 cgtggaaaat cttagccgaa taccagcacg aaatttttgg caacaacctc ctgacaatca
     3421 attatatggg cgcgttcggg cgcaggcttt ttctgacgcg gacgcttttt aacgccgacc
     3481 cgaattataa tttcgtccgg ttgacaaaca acgacgccga atccgatttt cacgcggcga
     3541 acgtccttta cgaaaagcgt tttgcgcaaa acttttcagt ctcgacgcgc tacacgctcg
     3601 ctaaatcgac cgacaatttt tcgcccggca atctgcggca gaatgttttc gccggcgagg
     3661 atttgagcga cgaacgcggc gcgtcggatt tcgacgtccg tcatcagctc agcgtctacg
     3721 gcgtttacga tattcagacg cctttccgca gcagcgcgat aaattttctc acccgcgact
     3781 ggacgatttt cgggtttgcc aacgcgcgca cgggttttcc gttgaatgtc acttacgccc
     3841 gcgtcaatga tttcggcgtc gagcttttgc gtcccgattt gatttcaaac gcgccgcttt
     3901 ataccggcgc gggcggcgga aaacaaatta atccagccgc tttttcgatt cccgctgccg
     3961 gaagacaggg cacgctgggc agaaacgctt tgcgcggatt tccgtttttt cagatggatt
     4021 tgggtctgga aagacgtttt cgtctcggcg gcgaatcgaa tctgcggctg acgatggaag
     4081 tttttaatgt gctgaacaac acgaattttg ccgatatgag cggcaatctc ggaacgctgg
     4141 cggcaaacga aagttttctg ccgaacagct atttcggcaa atccgcttcg actttcggca
     4201 gtcaaaactt cacgccgttt tatctttacg gcggcgcgcg gacaattcag ttttcggcta
     4261 aatttgtttt ttaaaaaatt tagccgcgcg gaaaagccgc gcgagaattg ccggcgggaa
     4321 cagggattta cagagataag aagaatgatt ttattatccg ctgtttaccg gcgcatcgca
     4381 tatttataat ttggcatatt tgtattaaat tcttctgtaa attgatttta aagcttcaag
     4441 cggtttgccc gtcgccgatt gaaaagcttt tgcaaattta cgaaaaagtt acttgaaaac
     4501 gccatcgccg agaaaatttt ggcggagata ggaaatcgta tcctgcaagg tttcctgcgc
     4561 atcgcgcggc tggaaattca acgtttcctc ggctttcgtc gaatcgaaat accagaaata
     4621 ttcggcttgc tcgacttctt tcggctggac gggcgaggtt ttgttccagt ttttaaaaac
     4681 cgattcaatc aggctcgcgc cggtcatcgc cagttttttc ggcacgcgca gcatcggcgc
     4741 cgaaactccg gaaagtcttt cgagccgccc gaaaaattcc ttaaagctca tattctcagc
     4801 gccgagcaga tatttctccc ggtgacttcc cttttcgagc gcctggacaa aagcctccgc
     4861 cgcatcgcgc gcgtcgacaa acgaaagccc gccgctcggc gtgtacggaa ttttgcgggc
     4921 gagaaaatcc agcacgggtt tcgtggagct taatctttcg tcctccgtgc cgagcagcag
     4981 agacggattc aggataacga gcttcaagcc tttgccgtca aaattttcga gcgccgtgcg
     5041 ttcctgataa tattttgaag cgtaataagc ccagcgcgag atgatttcga ttggcggcgg
     5101 gtaagtttcg tctaaaattt cctcggtttc cgaaacggca atcgttccgc tcgatgaagc
     5161 cagaatgaaa gctttcacgc tcgcctcttt cgccgcttcg cataaaatcc gcgttccctg
     5221 aacgtgaatt ttattcatcg cggcggcgtc ttcgttatcg cgcgagacct ttccggcgag
     5281 atgataaatc gccgaaacat ttctgaccgc ggcggcgacc gtctcgcgat tcgtcaccga
     5341 accttcggcg gcttcgacgc ccgcgtcttt catccattcg ggaacggacg aagccatcac
     5401 gcgcagattt ttctcgccgt cggctaaaaa ccggcggacg atgtgcgtcc ctaaaaatcc
     5461 cgttccgccg gtgattaaaa ttttcttctc ggtttttgct aatgcttttg tttttttcgc
     5521 catttatttt tcgcgtctca atcaaaattt ccgtgctgcg aaaagttata ttttcatttc
     5581 gattagtata aatacaataa aaaactttcc aaaac
//
LOCUS       DASWNE010000430         1540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_14872, whole genome shotgun sequence.
ACCESSION   DASWNE010000430 DASWNE010000000
VERSION     DASWNE010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14872"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            363..>1540
                     /locus_tag="VGB68_08640"
     CDS             363..>1540
                     /locus_tag="VGB68_08640"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEY0049336.1"
                     /translation="MKQILRAALLRNFVRFGFCLLLLQLVFSHTANAGFLYVYNETGN
                     SIYGYSVNERTGALSPLAGFPVVISGTGNNSSFKTDLLNIDPVNNRLYVISDGSDKVS
                     AYFINPATGALTALPFSPIQLPAGVYNSVQLNPSGSLLAVNELQNSTTRIYAVTQTSA
                     SLSQTLPFNVSNEIASNTIFSRDGNYFYQIAGGGTFFRGFNVDQTTGLMTEMFGSPFS
                     PSPGAGALTDSQGRIFGFFGSASPPIGRVFTTSQGVPAFVHQSPIANQIFITTVLHPN
                     ERYIAAVSGGNRVYSVRINGTGGGTTLTHAADAPSGGSNVRALVFNQAGNFVFGGGNR
                     ISTFNFFRDTGNITLNNVQPPETNGFGNVPGIAYLPTGIPVYLSGRITDAGGRPISGA
                     "
BASE COUNT          374 a          407 c          358 g          401 t
ORIGIN      
        1 atttttgtaa aatatcttaa attaatgctc ccgcgccgat ttgaacgatt gacctgccgc
       61 ttagaaatcg aaagagcgag gatttcagtt aatgccttaa actttcaaaa catagctaat
      121 ttaggtaata atataaccag ctcaacaaga taatacttca aattaacttt cggttagtcg
      181 ccggaagcgt aagacactat cttacatcat ataataggtt caaattgagc ccggagaatt
      241 ctccaaactc aaacatttgt ccgccgcgcc ctacatcgga caaatacgtg agcgaatgta
      301 aaaagtctaa taaataaacc gtctcggaaa cgaccctttt gagacaggag acctgtaata
      361 ccatgaaaca aattttacgc gcggctctac tcaggaattt cgttcgattc ggcttttgtc
      421 ttctactgct tcagttagtt ttctcgcaca cggcgaatgc cggttttctt tacgtttata
      481 atgaaacggg caacagcatc tacggctatt cggtcaacga aagaaccggc gcgctgtcgc
      541 cgctcgcggg ctttccggtc gtaatcagcg gcacgggcaa caactcttcc ttcaaaaccg
      601 atttactgaa tatcgacccg gtaaataacc gtttatacgt tatctccgac gggtctgaca
      661 aagttagcgc ctatttcatt aacccggcga ccggcgcgct tacggctctg cctttttcgc
      721 cgattcagct tcccgccggc gtctataatt cggtgcagct gaatccttcg ggttcgctgc
      781 tcgccgtcaa cgaactccaa aactcgacca cacgcattta tgcggttact cagacttcgg
      841 cgagtctttc gcagactctt ccgtttaacg tcagtaatga aatcgccagc aacacgattt
      901 tcagccgcga cggaaattat ttctaccaga ttgccggcgg cggcactttt tttcgcggat
      961 tcaatgtcga ccagacgacg ggcttgatga cggaaatgtt tggctcgcct ttcagcccgt
     1021 cgcccggcgc aggcgcgctg accgattcgc agggcaggat tttcggattt ttcggcagcg
     1081 catccccgcc catcggacgc gtttttacca ccagtcaggg cgttcccgcg tttgttcatc
     1141 aatcgcctat cgccaatcag atatttatta ctaccgttct gcatccgaac gaaaggtata
     1201 ttgccgccgt gagcggtggt aatcgcgtgt attccgtgcg aattaacgga acgggcggcg
     1261 gcacgacttt aacgcatgct gcggatgctc cgtccggcgg aagtaatgtc cgcgcgctgg
     1321 tttttaatca ggcgggaaat tttgtcttcg gcggcggtaa tagaatttct acttttaact
     1381 ttttcagaga taccggaaac attactttaa ataacgtgca gccgccggaa acaaacggct
     1441 tcgggaacgt gcccggcatt gcgtatctgc cgaccggaat tcccgtttac ctgagcggca
     1501 gaataaccga cgcgggcggt cgcccgattt ccggcgcgag
//
LOCUS       DASWNE010000431         3606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_684685, whole genome shotgun sequence.
ACCESSION   DASWNE010000431 DASWNE010000000
VERSION     DASWNE010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684685"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..461
                     /locus_tag="VGB68_08645"
     CDS             204..461
                     /locus_tag="VGB68_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049337.1"
                     /translation="MKLENIKRVEEVESVEILNFYPEKGWNILSTEKRVSGYSGAIEE
                     KIYFIIAWDKDEEPIYPEYRNEDELFKARNPLLLKNDENYI"
     gene            505..2079
                     /gene="pruA"
                     /locus_tag="VGB68_08650"
     CDS             505..2079
                     /gene="pruA"
                     /locus_tag="VGB68_08650"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF002852.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HEY0049338.1"
                     /translation="MQTQRVLDEFRNEPFTDYTKAENAEAMRAALEQVKSELGRDYPV
                     IVGGEKIDLPTKFESINPSNKTQVVGRFSDADTDAENLVEKAIDAASAAFELWKNVSA
                     EERAEYIFKIAAILRERKHYFSAWMVMEVGKTWAEADGDTAEAIDFCEFYGREMLRWA
                     QPQPVVPFPGEDNRIEYIPLGVGAIIPPWNFPLAIMAGMTLSAVVTGNTTVLKPSPDA
                     PMIAYKFMEVLEEVGLPAGVVNLVVGSGTRVGELMVGSPKVRFISFTGSKTVGLHIYE
                     QAARTQKGQRWIKRVVAEMGGKDAIIVADDADLDSAAQGVVSSAFGFQGQKCSACSRL
                     IVDEKVHDELIEKVVELTKKLKIGAPEEPDTNVAAVINQKSFDKATSMIQKGIEEGGK
                     ILVGGSGMSENGFYIEPTIIDDVQPQSTVEQEEIFAPVLAVIKAKDFDHALEIANDTE
                     YGLTGAVYSTSQGRLEKARREFHVGNLYFNRKCTGAMVGAHPFGGFNMSGTDSKAGGR
                     EYLLQFMQAKLVSQKI"
     gene            2192..3091
                     /locus_tag="VGB68_08655"
     CDS             2192..3091
                     /locus_tag="VGB68_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049339.1"
                     /translation="MKKNFFVKSFACLLIINLSISAIYGQKAKPIIFAVLNDGKTLEP
                     IALIEKGKLVPASDGGDEAKIKTQFTKTYYKPKTSYRLIFGGAVGGAVTVKSSDPTAE
                     CASNLAEVSTQSTKTKLSGFVMALATNAPPNSAISSRRQPTPDERAEISSLVRAEFVK
                     QKAAASALKNLKFHNMTALDVENDGKAEIVGTALVETSPTSRAILFFIADKNKAGKYA
                     FGFSEFKAMTQEEMMSGAAITAIDIGVYCELLLDVLEYDGDKTGEIFTYTQGLEGNGF
                     NAYRRSGGKWIRAFEGSNYHCGF"
     gene            3116..3517
                     /locus_tag="VGB68_08660"
     CDS             3116..3517
                     /locus_tag="VGB68_08660"
                     /inference="COORDINATES: protein motif:HMM:NF019932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0049340.1"
                     /translation="MSEELAEGCAPDDPVKQEILNLAEQWARALISNDAQKIGDFMSD
                     DWVIVGQNGITRKQDFLSSVESGDLTHETMEPVGAARVQIYGDTAVLTGRVTNNGHYK
                     GTPFSADEWTTDVFLRREGRWLCVISHITPV"
BASE COUNT         1022 a          790 c         1002 g          792 t
ORIGIN      
        1 gtccggaatt aatgtatggc ggtgttcacg gtgacgatgc gattgaagtc ttcaaggaac
       61 tttgcgaagt tgtagatgaa tgggttgaga ttttgaaaca agacggaaaa cctttgccgg
      121 aaccgaaagg ctttctttta aaagcagctt aatttttagc cgaaagattt agcaaacata
      181 caacagaagg acacaagcga attatgaagc tggaaaatat caaaagagtt gaggaagttg
      241 agtccgttga gatattaaac ttttaccctg aaaaaggttg gaatattctt tcaacggaaa
      301 aaagagtgtc cggttattca ggggcaatcg aagaaaaaat ttactttata attgcttggg
      361 ataaagatga agagccgatt tatcctgaat atcgtaacga agatgagttg tttaaagcga
      421 gaaatccact tttattaaaa aatgatgaga attatattta attaagtaac aactaataag
      481 ttgaaagaaa ataggattaa aattatgcaa acacagagag ttttagacga atttagaaac
      541 gagccgttta ccgattacac aaaagcggaa aacgccgagg cgatgcgcgc ggctttggaa
      601 caggtgaaaa gcgagctcgg gcgcgattat ccggttatcg tgggcggcga gaaaatcgat
      661 ttgccgacga aattcgagag catcaacccg tcgaacaaaa cgcaggttgt cgggcgtttt
      721 tccgatgccg atacggacgc ggaaaatctg gtcgaaaaag cgatcgacgc ggcgagcgcg
      781 gctttcgagc tttggaaaaa cgtttccgcc gaagaacgcg ccgaatacat tttcaaaatc
      841 gcggcgatac tgcgcgaaag aaagcattat ttttcggctt ggatggtgat ggaagtcggc
      901 aaaacgtggg cggaagccga cggcgacacg gcggaagcga ttgatttctg cgagttttac
      961 gggcgcgaga tgctgcgctg ggcgcagccg cagccggtcg ttccgtttcc cggcgaagac
     1021 aaccgcatcg aatacattcc gctcggcgtc ggcgcgatta ttccgccgtg gaattttccg
     1081 ctggcgatta tggctggaat gaccctgagc gcggtcgtga ccggaaacac aacggtttta
     1141 aagccttcgc cggacgcgcc gatgattgct tataaattta tggaagtgct ggaagaagtc
     1201 gggcttccgg cgggcgtcgt gaatttagtc gtcggttccg gaacgcgggt cggcgaattg
     1261 atggtcggca gtccgaaagt tcgtttcatc tcgtttacgg gttcaaaaac ggtcggtctg
     1321 cacatttacg agcaggcggc gcgaacgcag aaaggtcagc gctggataaa acgcgtcgtc
     1381 gcggaaatgg gcggcaaaga cgcgattatc gtggcggacg acgcggattt ggattcggcg
     1441 gcgcagggcg tcgtctcgtc ggctttcggt tttcagggtc agaaatgctc ggcttgctcg
     1501 cgcctgattg tggacgaaaa ggttcacgac gagctgattg aaaaagtcgt cgaattaacc
     1561 aaaaagctga aaatcggcgc tcccgaagag ccggatacta acgtcgcggc ggtcatcaat
     1621 cagaaatctt tcgacaaagc gacttcgatg attcagaagg gaatcgaaga aggcggaaaa
     1681 attctggtcg gcggcagcgg tatgagcgaa aacggctttt acatcgagcc gacgattatt
     1741 gacgacgtgc agccgcagag caccgtcgag caggaagaaa tttttgcgcc cgtgctggcg
     1801 gtgattaaag cgaaagattt cgaccacgcg ctggaaatcg ccaacgatac ggaatacggc
     1861 ttgacgggcg cggtttattc gaccagccag ggcagactgg aaaaagcgcg gcgcgaattt
     1921 cacgtcggaa atctttattt caaccgcaaa tgcaccggcg cgatggtcgg cgcgcacccg
     1981 ttcggcggct ttaatatgtc gggaaccgat tcaaaagccg gcgggcgcga atatctcctg
     2041 cagtttatgc aggcgaagct agtctcgcaa aaaatataat tcaatttcgt caacgaaaaa
     2101 tggtaaacgg caccgaaaaa tcaaataaaa tgtgttatct tttcggtgcc gtttaccatt
     2161 tttattttaa ttcaaaaaac gcacatcaaa tatgaaaaaa aactttttcg ttaaatcttt
     2221 cgcctgtctg ttaattatca acctgtcaat ttcggcaatc tacggacaaa aagccaagcc
     2281 gataattttt gccgtactaa acgacggcaa aacgctcgaa ccgattgccc tgattgaaaa
     2341 aggcaagctt gttccggcat cggacggcgg cgacgaggca aaaatcaaaa cccagtttac
     2401 gaaaacctat tacaaaccga aaacctcgta caggctgatt ttcggcggag ccgtgggcgg
     2461 agccgtgacg gtcaagagct cagacccgac ggcggaatgc gcgtctaatc tggcggaggt
     2521 ttcgacgcaa tcgacgaaaa cgaaattaag cggttttgtg atggctttag ccaccaacgc
     2581 gccgccgaat tcagcgataa gctcgcggcg gcagccgacg ccggacgagc gcgccgaaat
     2641 cagctcgctg gtgcgcgccg agttcgtcaa acagaaagcg gcggcaagcg ctttaaaaaa
     2701 tctgaaattt cataatatga ccgcgctcga cgtggaaaat gacgggaaag ccgaaatcgt
     2761 cggcacagcc ctggtcgaaa cttcgccgac gagccgcgcc attcttttct ttatcgccga
     2821 taaaaacaaa gccggaaaat acgctttcgg ttttagcgaa tttaaagcta tgactcagga
     2881 agaaatgatg agcggcgctg ccatcacagc catcgatata ggcgtctatt gcgagcttct
     2941 gctcgacgtg ctggaatacg acggcgacaa aacgggcgag atttttacat acacgcaggg
     3001 cttggaagga aacggcttca atgcctaccg gcgtagcggc ggcaaatgga ttcgcgcgtt
     3061 tgaagggtca aactatcact gcgggtttta gaaaaataat agtaagagga tgcggatgag
     3121 cgaagaactt gctgaaggct gcgcgccgga cgacccggtt aaacaggaaa tattaaattt
     3181 agcggagcaa tgggcgcggg cgctaatctc gaatgatgcg caaaaaatag gcgattttat
     3241 gagcgacgat tgggtaatcg tcggacaaaa cggaatcacg cgaaagcagg atttcttatc
     3301 ttccgtcgaa tccggcgatt tgactcacga gacgatggag ccggtcggcg cggcgagagt
     3361 gcaaatatac ggagataccg ccgttctgac cgggcgcgtg accaataacg gtcattacaa
     3421 aggcacgccg tttagcgccg acgaatggac gacggacgtt tttctgcgcc gcgaaggtcg
     3481 ctggctgtgc gtaatcagcc atatcacgcc ggtttgagcg atgtcggctg tagtgattat
     3541 ttgattaaca ggtcgagctt cgattaatac gagctgaaat tatcaaaggc aaaggcatag
     3601 atttta
//
LOCUS       DASWNE010000432         4595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_367158, whole genome shotgun sequence.
ACCESSION   DASWNE010000432 DASWNE010000000
VERSION     DASWNE010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367158"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1429
                     /locus_tag="VGB68_08665"
     CDS             <1..1429
                     /locus_tag="VGB68_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049341.1"
                     /translation="ANIGQFGKQGFDLLPTTRSQVYEGFSSESRMGTQAVMETRGLVA
                     TYKGKPINALYTSTCGGRTENSENIFEFNEPYLRGVECSLEGRRHFEPTLVKTVRLPA
                     RLRDERNVELVRQMSLYALNGFQLSTAQLNDEWFEDAPSESEVSNWLNQLAVRFGKTF
                     PSVNKETGKPAELARILAQMIYGDAYADTLLSEADINYQLSFDDAAEIPKERRADIAI
                     LLRDGYFTIHPDLTLKPNRAFSRARMLRLIQQIFDKKKWSIALQSGVTQASENGKLIV
                     KSGKTSRQLTVRPDVFLFRQFGESLYQVKETALVGGETVNFLTNSTGEVTYLEIKPAV
                     TTVTAEKMSPFTLWNVNLSASAVQSRLARYVRGMGSLIDVRIAQKGYSRRATDLEIVA
                     TNGTFHLKRGKIRSALKLREQLFVMNKRYDSTGKAVSYSFTGRGWGHGVGMCQYGAYG
                     LGKMGVKYDAILKHYYTGIDLTKAY"
     gene            complement(1863..4157)
                     /locus_tag="VGB68_08670"
     CDS             complement(1863..4157)
                     /locus_tag="VGB68_08670"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEY0049342.1"
                     /translation="MKKIFVCVIVLILQLNLAAQKTENFVRYVNPLIGTRKMGHTFPG
                     ATVPFGSVQLSPDTDQQPYIIGGKYNKDTYKYCAGYQYDDKQIVGFAHTHFSGTGHSD
                     LGDFLIMPTAGDLQLEPGDKNNPKTGFRSAFSHENETAEPNYYRVKLDDHDILAEMTT
                     TTRVGFHQYTFPQSDNAHIILDLMHGIYNYDDKNVWTFVRVENDRTIVGYRQTHGWAR
                     TRTVYFAMEFSKPFTNYGFKNFGAETYRGFWRKFDQTKNFPEMAGKQIRAYFDFKTAK
                     DEKIKVKFALSPVSTGGAMRNLRAEIPHWDFEKTKKDGQAVWNKELGKIAVTAETKGD
                     LVNFYTALYHTFLSPTVYMDSGGEYKGLDQNVHRARGFTNYTTFSLWDTYRALHPFFN
                     IVQPTRNREMIQSMLAHYDQSVHKMLPVWSHYANENWCMIGYHSVSVIADAIVKGNLK
                     GIDAQKALDASVQTARTKYYDGLQFYMSLGYVPEDKNSSSVSKTLEYAYDDWCIAQMA
                     KKLNRNDIYNEFIKRSENWKNVYDSSVGFMRPKLSGGNWREKFDPLQTHDQGFIEGNA
                     WNYSLYVPHNPAEMIAMMGGKKKFAARLDELFTMHLPDEFFAETEDITREGIIGNYVH
                     GNEPSHHVAYLYNWTDEPQKTQERVRMILKNQYQPAPDGLGGNDDCGQMSAWYLFSAL
                     GFYPVAPASDQYALGSPAIKTAALKLENGKILTIEARNQSDKNVYVEKVVVNGQTLDR
                     HYLTHAEIMNGGRITFYMSDKPKT"
     gene            complement(4310..>4595)
                     /locus_tag="VGB68_08675"
     CDS             complement(4310..>4595)
                     /locus_tag="VGB68_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049343.1"
                     /translation="DLGDFQLIVITFLAVAIYIVQVFAFLEVVQLSRFATLPDVDSAL
                     LTVFGLGHGSYLVKKAVGEGPVSQKSTTNPPVKAEPKIQTDVSPVEKDES"
BASE COUNT         1078 a         1184 c         1163 g         1170 t
ORIGIN      
        1 cgccaacatc ggacaatttg gaaaacaagg ctttgatttg ctgccgacga cgcgctcgca
       61 ggtttacgaa gggttttctt cagaaagccg gatgggcacg caagccgtta tggaaacgcg
      121 cggcttggtc gccacctaca aaggcaagcc gatcaacgcg ctttacactt cgacctgcgg
      181 cggcagaacg gaaaactcgg aaaatatttt cgaattcaac gagccttacc tgcgcggcgt
      241 cgaatgctcg ctcgaaggtc gccgtcattt cgagccgacg cttgtcaaaa ccgtccgcct
      301 gcccgcccgc ctgcgcgacg aaagaaatgt cgagctcgtc cgccagatgt cgctttacgc
      361 gctcaacggc tttcagcttt cgaccgcgca gctaaacgac gaatggtttg aagacgcgcc
      421 gagcgaaagc gaagtttcca actggctcaa ccagctcgcc gtgcgtttcg gcaaaacttt
      481 tccgagcgtc aataaagaga ccggaaaacc ggcggagctg gcgcgaattc tggcgcagat
      541 gatttacggc gacgcctacg ccgacacgct tttgagcgag gctgacatca attaccagct
      601 ttccttcgac gatgccgccg agattccgaa agagcgccgc gccgacatcg ccattttgct
      661 gcgcgacggc tatttcacga ttcatcccga tttgacctta aagccgaaca gagccttttc
      721 gcgcgccagg atgctgcgcc tgattcagca gattttcgat aaaaagaaat ggtcgattgc
      781 cctgcaatcg ggcgtcacgc aagcgagcga aaacggaaag ctcatcgtca aaagcggcaa
      841 aacaagtcgt caattaactg tgcgccccga cgttttcctg ttccgccagt tcggcgaatc
      901 gctttatcag gtcaaagaga ccgcgctggt cggcggcgaa accgtaaatt ttctgaccaa
      961 ttcgacgggc gaagtcacct atctggaaat caagcccgcc gtgacgaccg tgaccgccga
     1021 aaaaatgtcg ccgtttacgc tctggaacgt caatctgtcg gcgtcggcgg tgcagtcgcg
     1081 cctcgcgcgc tacgtgcgcg gaatgggttc gctgattgac gtccgcatcg cgcagaaagg
     1141 ttattcgcgc cgcgcgaccg acctcgaaat cgtcgcgacc aacggcacat ttcacctgaa
     1201 acgcggcaaa atccgctcgg ctctgaaact gcgcgagcag cttttcgtga tgaacaaacg
     1261 ctatgattcg accggcaaag ccgtttccta ttccttcacc gggcgcggct ggggacacgg
     1321 cgtcggaatg tgccagtacg gcgcttacgg actcggcaaa atgggcgtca aatacgacgc
     1381 gattttgaag cattactaca cgggaatcga tttaacgaag gcttattgac ggcaaatgaa
     1441 attatctcgt atatggagat gtgtgtccgc gagaaaacat ctctgcaacg cggaatgaat
     1501 taccgaaatg ctaaggacga attacatttc gaccacattc tgcctttttc gaaaggcgga
     1561 acttcaatca aagcggaaaa tgtgcaactt ctgtgcgcac gacataatct tgctgaaagc
     1621 gacaaaataa tttagctttc catacattac aagcacgatt tccgtcgcat tgcttttgtt
     1681 tcatcgccga aataacttat gagattttct cgcaatcaaa tcatcggctc gcttattttg
     1741 ctcggcgtga ttttgctgtc tgcatttatt cggattgtca tctaaaacaa cggaaaacgg
     1801 gtaacggaaa atagaaagcc tgtttcttaa ttttccatta cccgtttttc gctttccgtt
     1861 tcttacgttt tcggcttgtc gctcatataa aacgtaatcc gcccgccgtt cataatttcc
     1921 gcgtgcgtca ggtaatggcg gtcgagcgtc tgcccgttga cgacgacttt ctcgacgtag
     1981 acgtttttat cgctttgatt ccgcgcttca atcgttaaga ttttgccgtt ttcgagtttt
     2041 aaagctgcgg tttttatcgc cgggcttccc agagcgtact ggtctgaggc gggcgcgacc
     2101 ggataaaaac ccagagcgct gaaaagatac caagccgaca tctgcccgca atcgtcgttg
     2161 ccgcccaagc cgtcgggcgc gggctggtat tgatttttca aaatcatccg cacgcgttcc
     2221 tgcgttttct gaggctcgtc cgtccagttg taaagatacg cgacgtgatg cgaaggctcg
     2281 ttgccgtgaa cgtaatttcc gataattccc tcgcgcgtga tgtcctcggt ttcggcgaaa
     2341 aattcgtccg gcagatgcat cgtgaaaagc tcgtcgaggc gcgccgcaaa tttctttttg
     2401 ccgcccatca tcgcaatcat ttccgccgga ttgtgcggca cgtaaagact gtaattccac
     2461 gcgttgcctt cgataaatcc ctggtcgtgc gtttgcagcg ggtcgaactt ttcgcgccag
     2521 tttccgccgc ttaatttcgg gcgcataaag ccgaccgaag aatcgtaaac atttttccag
     2581 ttttccgagc gcttgataaa ttcgttataa atatcgtttc gattcagctt tttcgccatt
     2641 tgcgcgatgc accaatcgtc gtatgcgtat tccagagttt tcgagacgga cgaggaattt
     2701 ttgtcttcgg gcacgtagcc gagcgacata taaaattgca gaccgtcgta atatttcgtc
     2761 cgcgccgtct gcacggaagc gtccagcgct ttctgcgcgt caatgccttt gagattgcct
     2821 ttgacaatgg cgtcggcgat gacggaaacc gaatgataac cgatcataca ccagttttcg
     2881 ttggcgtaat gcgaccagac gggcagcatt ttatgcacgc tctggtcgta atgcgccagc
     2941 atcgactgaa tcatctcgcg gttgcgcgtc ggctggacga tattgaaaaa cgggtgcagc
     3001 gcgcgatacg tgtcccagag cgaaaaggtc gtgtaattgg taaacccgcg cgcccggtgc
     3061 acgttctggt ctaagccttt atactcgccg cccgagtcca tataaacggt cggcgacaaa
     3121 aacgtgtgat aaagcgcggt gtaaaaatta accaaatcgc ctttcgtctc cgccgtgacg
     3181 gcgatttttc cgagttcttt attccaaacc gcctgcccgt cttttttagt tttttcaaaa
     3241 tcccagtgcg ggatttcggc gcgcagattt cgcatcgcgc cgccggtcga aacgggcgaa
     3301 agcgcgaatt ttactttgat tttttcgtct ttcgcggttt taaaatcaaa gtatgcgcga
     3361 atttgtttgc ccgccatctc gggaaaattt ttcgtctggt cgaatttgcg ccagaatccg
     3421 cgataagttt ccgcgccgaa attcttaaaa ccgtaattcg taaacggctt ggaaaattcc
     3481 atcgcgaaat agacggttct ggttcgcgcc cagccgtgcg tctgccggta gccgacgatc
     3541 gtccggtcgt tttccacgcg gacaaaagtc cagacgtttt tgtcgtcgta gttgtagatg
     3601 ccgtgcatca aatcgagaat gatgtgggcg ttgtcgcttt gcgggaaagt gtattgatga
     3661 aagccgactc tggtcgtggt cgtcatttcc gctaaaatgt cgtggtcgtc cagtttgacg
     3721 cgataataat taggctcggc ggtttcgttt tcgtgcgaga aagccgacct gaagcctgtt
     3781 ttcggattat ttttatcgcc cggctcgagc tgcaaatcgc cggcggtcgg cataatcaga
     3841 aaatcgccca aatccgaatg tcccgtgccg ctgaaatgcg tgtgcgcaaa gccgacgatt
     3901 tgtttatcgt cgtattgata accggcgcag tatttgtatg tgtccttgtt gtatttcccg
     3961 cctataatat agggctgctg gtcggtatcg ggcgaaagct gaaccgagcc gaaaggcacg
     4021 gtcgcgcccg gaaacgtgtg tcccatcttt cgcgtcccga taagcggatt gacgtatcga
     4081 acgaaatttt ccgttttttg cgccgctaaa tttaattgca gaatcagaac gataacgcag
     4141 acaaagattt ttttcatttt gtttttcgaa tcggtcggat atactcgcga tttttagata
     4201 ctatacgcaa ccggtaatga ttaaggcaaa agaaaaaggc gaaacttaga gagcgtttct
     4261 tagtttcgcc ttttcctttt gtttttttat ccgggtaatc ggtaataaat cagctttcgt
     4321 ctttttcaac cggcgaaaca tcggtttgta ttttgggctc agccttgacc ggcggattgg
     4381 tcgtgctttt ttgagacaca ggaccttcgc cgacggcttt tttgaccaga tacgagccgt
     4441 gaccgagccc gaaaaccgtt aaaagagccg aatcgacatc cggcaaagtc gcgaagcgag
     4501 acagttgaac gacttcgaga aaagcgaaga cctggacaat ataaatggcg acggcgagaa
     4561 atgtaatgac gattaactga aaatcgccca aatcc
//
LOCUS       DASWNE010000433         1903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_191628, whole genome shotgun sequence.
ACCESSION   DASWNE010000433 DASWNE010000000
VERSION     DASWNE010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191628"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /gene="malQ"
                     /locus_tag="VGB68_08680"
     CDS             <1..1028
                     /gene="malQ"
                     /locus_tag="VGB68_08680"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF011080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEY0049344.1"
                     /translation="LRLRDEAALNRERERLREEIQAQKFYQFLFFRQWLAIKDYAAAK
                     NIKIVGDVPIFIALDSADVWCSQSNFKLNADGSPQVVAGVPPDYFSQTGQLWGNPIYD
                     WEKMLADGFGWWIERVRHTLKTVDIVRVDHFRGFAAAWEVPGGDKTAENGRWVNVPGK
                     ALFDALKNALGDLPFWAEDLGVITPDVEELRDSFGFPGMRILQFAFGGDTKNHDLPHN
                     YIKNCVAYTGTHDNDTTVGWFQSQAGAGSTRDDSQIRREHDFCLKYLRSDGAEIHWDF
                     IRAIWASAANTAITPMQDLLGLGSVARMNLPASSSGNWYWRFQDGDLSDEIVGRLKEL
                     TEIYGRI"
     gene            1025..1588
                     /locus_tag="VGB68_08685"
     CDS             1025..1588
                     /locus_tag="VGB68_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049345.1"
                     /translation="MSGKFSEKIPSFVWISIIVAAVALFIGAIMGGFYALAFVEGFAS
                     LILLLCGWAAFRAGNNSPQLESSSFGIALVISFFALMGMSLDQPGNYFYNKPVEWIFC
                     PAESRLERGVISTGTRGGRGVSVSQNFTCAADQSGEVLRKISTFEQLAVRFFEYVLLG
                     YVLLGLSRVYTRIKSSLKAGRKPVAGG"
     gene            1608..>1903
                     /locus_tag="VGB68_08690"
     CDS             1608..>1903
                     /locus_tag="VGB68_08690"
                     /inference="COORDINATES: protein motif:HMM:NF020653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclomaltodextrinase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0049346.1"
                     /translation="MKNKLRCLVFLLLTAHCSLLAAYAQAPSVEKIDPPSWWANMTIN
                     PVRILLRGKNLNAKTVWTYTSALKASNIKASANGNYLFFDLTIAKNAKPGKYKIS"
BASE COUNT          420 a          463 c          548 g          472 t
ORIGIN      
        1 ctttgcgcct gcgcgacgaa gccgctctga accgggagcg cgagcgcctg cgcgaggaga
       61 ttcaggcgca gaaattttat cagtttttgt ttttccggca atggctggcg attaaagatt
      121 acgccgccgc gaaaaacata aaaatcgtcg gcgacgtgcc gatttttatc gcgctcgatt
      181 cggctgacgt ctggtgcagc cagtcgaatt ttaaattaaa cgccgacggc tcgccgcaag
      241 tcgtcgccgg tgttccgccg gattattttt cgcaaaccgg acagctctgg ggcaatccga
      301 tttacgactg ggaaaaaatg ctggcggatg ggttcggctg gtggattgag cgcgtccggc
      361 acactctgaa aacggtcgac atcgtgcgcg tcgaccattt tcgcggtttt gcggcggctt
      421 gggaagttcc gggcggcgat aaaacggcgg aaaacgggcg ctgggtcaac gtgccgggca
      481 aggcgttatt cgacgcgctg aaaaacgctc tcggcgattt gcctttctgg gcggaagatt
      541 tgggcgtgat tacgcccgac gtcgaagaat tgcgcgattc gttcggcttt cccggaatgc
      601 ggattttgca gtttgccttc ggcggcgata cgaaaaatca cgatttgccg cacaattata
      661 taaaaaactg cgtcgcctat accggcacgc acgacaacga tacgaccgtc ggctggttcc
      721 aatcgcaggc gggcgcgggc tcgacgcgcg acgattcgca gattcgccgc gagcacgatt
      781 tttgtttgaa atacctgcgc tcggacggcg cggaaattca ctgggatttt attcgcgcga
      841 tttgggcttc ggcggcaaac acggcaatta cgccgatgca ggatttattg ggtctgggca
      901 gcgtagccag aatgaatctg cccgcgtcga gttcgggaaa ctggtactgg cgctttcagg
      961 acggcgattt atcggacgag attgtcggca gattaaaaga attgaccgaa atttacgggc
     1021 gaatatgagc ggaaaatttt ctgaaaaaat tccttctttt gtttggattt cgataatcgt
     1081 cgccgccgtt gcgcttttta tcggcgcgat tatgggcggt ttttacgctc tggcttttgt
     1141 cgaaggcttt gcttcgctga ttctgctgct ttgcggctgg gcggcttttc gcgccggaaa
     1201 taattcgccg cagctggaaa gcagcagctt cggaatcgcg ctggttatca gctttttcgc
     1261 gctgatggga atgtcgctcg accagccggg aaattatttt tataacaagc cggtggaatg
     1321 gattttctgc ccggcggaaa gccgtctgga gcgcggcgtc atcagcaccg gcacgcgcgg
     1381 cggcagaggc gtttcggttt cgcagaattt cacctgcgcg gctgaccaga gcggcgaggt
     1441 tttgcgaaag atttccactt tcgagcagct tgccgtcagg ttttttgaat acgttctgct
     1501 cggctacgtt ctgctcggtt tgagccgcgt ttacacccga ataaaatcat cgttaaaggc
     1561 tggacgaaag ccggtcgcgg gcggttgaat ttgaaataaa ttgaattatg aaaaataaat
     1621 tacgctgtct cgttttctta ctgcttaccg ctcactgctc gctgctcgct gcttacgcgc
     1681 aagcgccgtc ggtcgaaaaa atcgacccgc cgagctggtg ggcgaatatg acgataaatc
     1741 cggttcgcat tctgctgcgc gggaaaaact tgaatgcgaa aactgtctgg acatacacca
     1801 gcgctttgaa agcgagcaat attaaagcga gcgccaacgg taattatctt tttttcgatt
     1861 taacgatagc gaaaaacgcc aaaccgggaa aatataaaat ttc
//
LOCUS       DASWNE010000434         2773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_15139, whole genome shotgun sequence.
ACCESSION   DASWNE010000434 DASWNE010000000
VERSION     DASWNE010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15139"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..1409)
                     /locus_tag="VGB68_08695"
     CDS             complement(183..1409)
                     /locus_tag="VGB68_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049347.1"
                     /translation="MKTNKFGILTLILIILAMSAVVRAQCSDDSPIEKIESNAAQISG
                     ASDVIFSPSGKSMIVQLSWGFYLLPTENLDAAGAGFKESRFACLEGSAKGFLPSGKIV
                     FAGRRGLYAIDPATKKTEPIYQITAAEEEQGNYLIDGEMVSVSENLILSGDGDYDLGL
                     KKGNILRFDVKRRGVSRRAPIRGFQNPMLSPGGKYILYEHNGESGGNTDFYDIAADRN
                     YPLAGRFNLKRVFPKYKRTFERPIAWISPKRFLAQVEENQTSPSDKYKADSKPWLVLF
                     DIAGKIVWKRQMTFELIPPYFHQLSATKALMENDGGVYEISLTSGKSELILPYSEGKA
                     ISISPDKKQIAYFDIKHLYIASLDGKNAKSVLALPYDWTGVSAYKEMGAPPPLWSPDG
                     TRLVIIDKSRALFVRL"
     gene            complement(1396..2397)
                     /locus_tag="VGB68_08700"
     CDS             complement(1396..2397)
                     /locus_tag="VGB68_08700"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HEY0049348.1"
                     /translation="MRKSILKIVIALSIIAASFAPVPAQKKSPQEKLPKTRDVTIYVY
                     QRPAETSGEFFGALVPVRRTIIDYRPLDAALKLLLRGANQNEQKRNLTNFIFGLEFHS
                     ARVRNKTAQINFKFDNPEIALESWEGGGFDHENFVKAVNRTARQFPGIERVSICVDGM
                     KNYTNFDRSNSGKCAFPMFPPAPPRVVPPDEFWDAEIYLNHSSAPVLPMNARGSLPVK
                     RRVKAADFLNETLKAFDAGATDAERKQGWSPATYGAHFVSARLKNKKAFVRFTMPAGA
                     KFPDRFSPLEFNFAVTDTVLQFWNEIDEIEVCLDGIIDFYAEYPERIPCEQEQNENE"
     gene            complement(2419..>2773)
                     /locus_tag="VGB68_08705"
     CDS             complement(2419..>2773)
                     /locus_tag="VGB68_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049349.1"
                     /translation="AADFNGDRFRDEAWILISTEREDGLGLFVFFGSKKGLGEARLLG
                     ESGIAPQAMHIRALKPGKYRTACFYGYWDCTEEEPQLLRLKTSGIIYAMIGESTAVNY
                     WNANNRKFYVAQLTH"
BASE COUNT          674 a          724 c          665 g          710 t
ORIGIN      
        1 agatacgacc ggcacaatcc tttggaagtc gcgctcgtga tttaaagcgc gccgcgaaaa
       61 atcgataaac cgcaaaatta ataaagcggc tttaaagaac ttgaaagccg ctttatgtct
      121 gcaaatcatt ttattaaata atccgccaat tatgttttcc gacaatagcg ttttatttac
      181 gattaaagcc ggacgaacaa tgctctgctt ttatcgatga ttacaaggcg cgtgccgtcc
      241 ggcgaccaaa gcggcggcgg cgcgcccatc tctttgtatg ccgaaacgcc tgtccaatcg
      301 tatggtaaag ccaaaaccga tttcgcgttt tttccgtcga gcgaagcgat atataaatgt
      361 ttaatatcga aatacgcgat ttgttttttg tcggggctga ttgaaatcgc ctttccttcc
      421 gaataaggga gaatcaactc cgattttccg ctcgtcagag aaatttcgta aactccgccg
      481 tcgttttcca tcagagcctt ggtcgcgctc aattgatgaa aatacggcgg aatcagctcg
      541 aaggtcattt gtcgcttcca gacgattttt ccggcgatat cgaataagac cagccacggt
      601 tttgaatcgg ctttgtattt gtcggacggt gaggtttgat tttcttcaac ctgcgccaga
      661 aagcgttttg gcgaaatcca ggcaatcgga cgctcgaaag tgcgcttgta tttcggaaag
      721 actcgtttga gattaaaacg cccggcaagc ggataattcc ggtcggcggc gatgtcgtaa
      781 aaatccgtgt tgccgccgct ttcgccgttg tgttcataca aaatatattt gccgcccggc
      841 gaaagcatcg gattttgaaa accgcgaatc ggagcgcggc gcgaaacgcc tcggcgtttt
      901 acgtcgaacc gcaaaatatt gccttttttc aatcccaaat cataatcgcc gtcgccggag
      961 aggattaaat tttcagaaac agagaccatt tcgccgtcga ttaaataatt gccctgctct
     1021 tcctcggcag cggtaatctg gtaaatcggc tctgtttttt tcgttgccgg gtctattgcg
     1081 taaagcccgc gccgacccgc aaacactatt ttccccgacg gcagaaaacc tttcgccgat
     1141 ccttcaaggc aagcgaagcg cgattctttg aaaccggctc cggcggcgtc gagattttcg
     1201 gtcgggagca gataaaagcc ccagctcaac tggacaatca tcgattttcc ggacggcgaa
     1261 aatatgacgt cggaagcacc ggaaatttgt gccgcgttcg attcgatttt ttcgattggc
     1321 gaatcgtcgg aacactgcgc ccgaacgaca gccgacatcg ccaatataat taagataagc
     1381 gttaaaattc caaatttatt cgttttcatt ttgctcctgc tcgcacggta tccgctccgg
     1441 atactcggcg taaaaatcga taatgccgtc cagacaaact tcgatttcat caatttcatt
     1501 ccaaaactga agaaccgtat cagtgaccgc gaaattaaat tcgagcggcg agaatctgtc
     1561 cgggaatttc gcgcccgcag gcatcgtgaa acgcacgaag gcttttttat ttttaagccg
     1621 cgccgaaaca aaatgcgcgc cgtaggtcgc cgggctccag ccttgtttgc gctcggcgtc
     1681 ggtcgcgccc gcatcgaagg ctttcagcgt ttcgtttaaa aaatcggcgg ctttgacacg
     1741 ccgtttgacc ggcagcgagc cacgcgcgtt catcggcaaa accggcgcgc tcgaatgatt
     1801 gagataaatt tccgcgtccc aaaattcgtc cggcgggacg actcgcggcg gcgcgggcgg
     1861 aaacatcgga aacgcgcatt tgccggaatt cgaccggtcg aaattcgtgt aatttttcat
     1921 gccgtccaca caaatcgaaa cgcgctcgat gcccggaaac tgcctggctg tccggttgac
     1981 ggctttgaca aaattttcgt ggtcaaagcc gccgccttcc caactctcca aagcgatttc
     2041 cggattgtcg aatttgaaat tgatttgcgc cgttttgttt ctgacgcggg ctgaatggaa
     2101 ttccagaccg aaaatgaaat tcgtcagatt ccgcttttgc tcattctgat tcgcccctct
     2161 gagcagcagc tttaaagccg cgtcgagcgg acggtaatcg atgatagtgc gccggacggg
     2221 gacgagcgcg ccgaaaaatt ctccgcttgt ttcagccggt ctctgataaa cataaatcgt
     2281 aacgtcacgc gttttcggca gcttttcctg cgggctcttc ttctgcgccg gcacgggcgc
     2341 gaaactcgcg gcaataatgg acaaagcaat gacgattttt aaaattgatt tcctcatatg
     2401 gcttaaaatt tactctgttt aatgcgttaa ctgtgcgacg taaaatttcc tgttatttgc
     2461 attccagtaa ttaacggcgg tcgattcgcc gatcatcgcg tagatgatgc cgctcgtttt
     2521 cagccgcaaa agctgcggtt cttcttccgt acaatcccaa tagccgtaaa aacatgccgt
     2581 ccggtatttt cccggtttca gagcgcgaat gtgcatggct tgcggcgcaa tgcccgattc
     2641 tccgagcagc cgcgcttcgc ccaagccttt tttcgagccg aaaaaaacga acagaccgag
     2701 tccgtcttcc cgctcggtcg aaatcaaaat ccacgcttcg tcacgaaaac ggtcgccgtt
     2761 aaaatccgcc gct
//
LOCUS       DASWNE010000435         1996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_297818, whole genome shotgun sequence.
ACCESSION   DASWNE010000435 DASWNE010000000
VERSION     DASWNE010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297818"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..1026)
                     /gene="hemH"
                     /locus_tag="VGB68_08710"
     CDS             complement(40..1026)
                     /gene="hemH"
                     /locus_tag="VGB68_08710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEY0049350.1"
                     /translation="MQEKIGVVLLNLGGPDSLEAVEPFLFNLFNDPDIINFPLSFLFR
                     KRLAKLISSKRHPRIQEQYKQIGGKSPLKDFTLRQANLLERKLNGKIPAKVYVAMRYW
                     HPLTEEALDEIEKDGVKKVVLLPLYPQYSKATTESSAKEWQKQLALRENPAKLEWSLI
                     ESYYDFPPYADAFVERINQGLEKFPAEKRNDVHILFSAHGTPMKLVRAGDPYSGHIKK
                     TVEAVVQRGNFSQPNSLCYQSKVGPQKWLKPSTPETIAALAAQGVKNMLVVPIAFASD
                     HLETLFEVGIEFRHLAQNAGVEQFEVTEGLNYSEKFIDALAQLVLQKLEKEN"
     gene            complement(1185..>1996)
                     /gene="hemN"
                     /locus_tag="VGB68_08715"
     CDS             complement(1185..>1996)
                     /gene="hemN"
                     /locus_tag="VGB68_08715"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEY0049351.1"
                     /translation="ITRQTVTWARELGYKSVNIDLIYGLPHQTLPEFAESVEKVIDIS
                     PDRIAVFNYAHVPWLKKHQNVIKVEDTPSPDERLQILRMTIEKLIAAGYEYIGLDHFA
                     KPSDELAIAQKNNTLYRNFQGYSTKAGCDVYAFGLSAISQFQNIYAQNLKNLKDYYKR
                     VESGEAATNVGYRMTPDDHIRKETIMQLMCHLEIDKRDIEAKFNIDFEDYFAADIKKL
                     DVFLRDGLLENNAEKIKIVGSGILIIRNVAMCFDAYLEKMMKEKPVFSKTV"
BASE COUNT          461 a          484 c          472 g          579 t
ORIGIN      
        1 gcctgctttt aatctgcgtc tgtccgcgtt catctgcggt taattttcct tctctaattt
       61 ttgcaaaacc aactgcgcca gcgcgtcgat aaatttttcc gaataattca agccttcagt
      121 tacttcaaac tgttcgacgc ccgcgttttg cgccagatgc cgaaactcga tgccgacttc
      181 gaacagcgtt tcgagatggt cggaagcaaa ggcaatcggc acgacgagca tatttttgac
      241 gccttgcgcg gcgagcgcgg caatcgtttc cggcgtcgaa ggcttaagcc atttttgcgg
      301 tccgactttc gattgataac aaagactgtt cggctgcgaa aaattgccgc gttggacgac
      361 cgcttcgacg gtttttttga tgtgtccgga ataagggtcg ccggcgcgga cgagcttcat
      421 cggcgttccg tgcgcgctga aaagaatgtg cacgtcgttt cgtttctctg ccggaaactt
      481 ttccagacct tgattgatgc gctcgacaaa agcgtccgcg tacggcggaa aatcgtaata
      541 agattcgatt aagctccatt cgagcttcgc cggattttct cttaaggcga gctgtttttg
      601 ccattctttc gcgctcgatt cggtcgtcgc tttggaatat tgcgggtaaa gcggcagtag
      661 gacgactttt ttaacgccgt ctttttcgat ttcgtccagc gcctcttcgg tcaacggatg
      721 ccagtaacgc attgcgacgt aaacttttgc gggaattttc ccgtttagtt tacgctccag
      781 aagatttgcc tgccggagcg tgaaatcctt gagcggagat tttccgccga tttgtttata
      841 ttgctcctgg atgcgcggat gccgttttga cgaaataagc ttcgccagcc tttttctgaa
      901 caaaaagctc agagggaaat tgataatgtc cgggtcgttg aaaagattga acagaaaagg
      961 ctcgaccgct tcgagcgagt caggtccgcc gagatttaat aaaactacac cgattttttc
     1021 ctgcattttt aaaaccaaat ttaaccgcag acgtacgcga atgaacgcag agataaatat
     1081 cgatggattt aaccggacgg tcagaattca gcaggcttcg cgcctcgata aaatcatttt
     1141 ctcatccgtg tctatctcgc gtttatccgc ggctaaaaaa ttcctcaaac cgtcttcgag
     1201 aaaacgggtt tttccttcat cattttctct aaatacgcgt cgaaacacat cgctacgttt
     1261 ctgataatca gaattcccga accgacgatt ttgattttct ccgcattgtt ttccaataat
     1321 ccgtcgcgca aaaatacgtc taattttttt atgtcggcgg cgaaataatc ttcaaaatcg
     1381 atattgaatt tcgcttcgat gtcgcgcttg tcgatttcca gatgacacat cagctgcata
     1441 atcgtttcct tgcggatgtg gtcgtccggc gtcattcgat aaccgacatt ggtcgccgcc
     1501 tcgcccgatt cgactctctt gtaataatcc tttaaattct ttaaattctg cgcgtagatg
     1561 ttttgaaact ggctgattgc cgacagcccg aaagcgtaaa cgtcgcagcc cgctttggtc
     1621 gaataaccct gaaaattgcg gtaaagcgtg ttgtttttct gcgcgattgc cagctcgtcc
     1681 gaaggcttgg cgaaatggtc gagaccgatg tattcgtagc ctgccgcaat aagtttttca
     1741 atcgtcattc gcaaaatctg caagcgttcg tcgggcgaag gcgtgtcttc gactttgatg
     1801 acgttttgat gttttttgag ccacggaacg tgcgcgtaat tgaaaacggc gatgcgatca
     1861 ggcgaaatgt cgattacttt ttcgaccgat tcagcgaatt ccggcaaagt ttgatgcggc
     1921 aagccgtaaa tcaaatcgat gttgacgctt ttatacccga gctcgcgcgc ccacgtaacg
     1981 gtttggcgcg taattt
//
LOCUS       DASWNE010000436         3393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_473439, whole genome shotgun sequence.
ACCESSION   DASWNE010000436 DASWNE010000000
VERSION     DASWNE010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473439"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..582
                     /locus_tag="VGB68_08720"
     CDS             55..582
                     /locus_tag="VGB68_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049352.1"
                     /translation="MKEKHIQISGICLTLLYGLFVVWLYIAEPKSLVELPTKAQQTID
                     KATTTAQVMTNTYEIDRAQFDEGLTAFRQDNFVRARDAFSKADTENRDAKTQFYIAYS
                     FYRQGWGRISNDDTLFRQGLEAVSRVTALDNNFKSDDAGLQLKTPVELKAELEQGLQV
                     TAADFNPLKVLRERK"
     gene            586..1494
                     /locus_tag="VGB68_08725"
     CDS             586..1494
                     /locus_tag="VGB68_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049353.1"
                     /translation="MEKGKRQNEETERTDLPVVDGELLEVLTRKNAEFSDEEETVVHE
                     EETIVRGAQKPAAAANRKPQLTKIQVPLTDKGKAVSQKSLLQYLLLPFIFLTVTLFGG
                     LRFGETDNAFLFLKPPLICLIFASILLLLFFRANLIRLEGWFSEDFPTVKIFANGLIL
                     LTLFTASVQVFNALLPERGLPFWVVAFCFFWTLWNNIFAEFNTKRLLQSLGSLFGLAF
                     VVKYLILAYLAAPSSGSWWQGILQNPTQEAFTWLLDLPKFAPATGYIQFFTLILYLIG
                     LFLLKPQMNAETASNEALSLKRIKPR"
     gene            complement(1667..1963)
                     /locus_tag="VGB68_08730"
     CDS             complement(1667..1963)
                     /locus_tag="VGB68_08730"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEY0049354.1"
                     /translation="MRLDLYLKASRLVLRRALANELCDAGLVKVNAAPAKPSRDVKTN
                     DEIEIKRRNKLTTIRVLQVPEKKQISKQDAPNLYEILSEETLEDSVFDFESPEN"
     gene            complement(2067..3119)
                     /locus_tag="VGB68_08735"
     CDS             complement(2067..3119)
                     /locus_tag="VGB68_08735"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY0049355.1"
                     /translation="MKFINSAIFLVAVLFFPVFSAVAQESEPVVIDEVVAQVNEGVVT
                     LSRIKREIKDATDALVRQGKTPEVAKAEIESRQGELIANIINEELILQKGKELGIDVE
                     GDINQRFLQIMKEQNIKTLDALKGEMRKAGIDPEELRESWRKQITRDYVLQREVDSKI
                     YFGLSNKEIKDYYEKNKVKFSKPETVTLSEIFLSFAGRDENAVMEKARQLVAQLRGGA
                     DFAKLAVENSERPNVKDTKGKLQTFKVAELNETFAAPLKTVKIGDVTDPIRIDEGIEI
                     LRVDSRQAASTESVFDDDEVRKAITYERLPAERKKFMATLRKDSYIKISESYKPIVAP
                     VLFEDDRKAEVKKPTE"
BASE COUNT          807 a          809 c          815 g          962 t
ORIGIN      
        1 atatctgcta aatatttatg tttattaaag ttagcgagta gctgaaaaat atagatgaaa
       61 gaaaagcata tccaaatttc aggcatttgt ttgacgcttc tttacggctt gtttgttgta
      121 tggctgtata tcgccgagcc gaaaagtctg gtcgagctgc cgaccaaggc gcagcagacg
      181 attgacaaag cgacgacgac cgcgcaggtg atgaccaaca catacgaaat cgaccgggcg
      241 caattcgacg aaggcttgac ggctttccgg caggataatt tcgttcgggc gcgcgacgct
      301 ttctcaaaag ccgatacgga aaaccgcgat gcgaaaacgc agttttatat cgcatacagc
      361 ttttaccggc agggctgggg cagaatttcc aacgacgaca cgcttttcag acagggcttg
      421 gaagcggtca gccgcgtcac ggctttagac aataatttta aatccgacga tgccggtttg
      481 cagcttaaaa cgccggtcga gcttaaagcc gagcttgaac agggcttgca ggtcacggcg
      541 gcggatttca acccgctgaa agttttgcgg gaaagaaaat agcaaatgga aaaaggcaaa
      601 aggcaaaatg aagaaaccga gcggactgat ttgcccgtcg tcgacggcga attgctggaa
      661 gttttaacgc gcaaaaacgc cgaattttcc gacgaagaag aaaccgtcgt gcacgaggaa
      721 gaaacgatag tgcgcggcgc tcaaaagccc gcggcagccg ccaatcgaaa accgcagcta
      781 accaaaatcc aggtgccttt gactgataag ggaaaagcag tcagccagaa atctcttctg
      841 caatatctgc tgctgccttt tatttttctg accgtgacgc ttttcggcgg cttgcgtttc
      901 ggcgagacgg acaacgcttt tctgtttctg aaaccgccgc tcatttgcct gattttcgct
      961 tcgattttgc tgctgctttt ctttcgcgcc aatttgattc ggctcgaagg ctggttttcg
     1021 gaagattttc cgacggtcaa aattttcgcc aacggcttga ttttactgac gctttttacg
     1081 gcttcggtgc aggttttcaa cgcgcttttg ccggagcgcg gcttgccgtt ctgggttgtc
     1141 gctttctgtt ttttctggac gctctggaat aatattttcg ccgaattcaa cacaaaaaga
     1201 ttgctgcaaa gcctgggcag cctgttcggg ctggcgttcg tcgtcaaata tttaattctc
     1261 gcctatctcg ccgcgccgtc gtctggaagc tggtggcagg gaattctgca aaacccgacg
     1321 caggaagctt tcacgtggct tctggatttg ccgaaattcg cgcccgcgac cggctatatt
     1381 caattcttca cgctgattct ttatctaatc ggcttgtttt tactcaagcc gcagatgaac
     1441 gcggaaacgg cttcaaacga agcgctttcg ttaaagcgaa ttaagccgcg ttaaagcaaa
     1501 acgcggtttc acggaaagaa aatttaattt cgtcgagaga aaactcgatt tcgttaaagc
     1561 gaagcctgat ttcatcgaaa gaaaacttaa tttttttgtt aagacaaaac tggatttcgt
     1621 taaattaaaa ccggatttcg tcaagacaaa atccggtttc ggcaggctag ttttcaggtg
     1681 attcgaagtc gaaaaccgaa tcttccagcg tttcttccga cagaatttcg tagagattcg
     1741 gcgcgtcctg ttttgagatt tgtttctttt ccggaacttg cagaacgcgg attgtggtca
     1801 gtttattgcg gcgtttgatt tcgatttcgt cattcgtttt gacatcgcgc gaaggcttgg
     1861 cgggcgcggc gttgactttc accagtccgg cgtcgcaaag ctcgttggcg agcgcgcggc
     1921 gcaatacaag acggctggct tttaaataaa ggtctaaacg cataaattta actgataatt
     1981 gataactgat aactgatagt agaaaattta aagctcgctt ttactttcca ctatcaatta
     2041 tcagttatca actatcagtt attcggttat tcggtcggtt ttttcacttc cgctttacgg
     2101 tcgtcttcaa aaagaacggg cgcgacaatc ggtttatagc tttcggaaat ttttatatac
     2161 gagtcttttc ttaaagtcgc cataaacttt ttgcgttctg cgggcaggcg ctcgtaagtt
     2221 atcgccttgc gcacctcgtc atcgtcgaaa accgattcgg tgcttgccgc ctgacggctg
     2281 tcgacgcgca gaatctcgat gccttcgtca attcgaatcg ggtcggtcac gtcgccgatt
     2341 ttgacggttt tcagcggcgc ggcaaacgtt tcgttcagtt ccgcgacctt gaaagtttgc
     2401 agcttgcctt tcgtgtcttt gacgttcggg cgctccgagt tttcgacggc gagcttggca
     2461 aaatccgcgc cgccgcgcaa ttgggcgacg agctgtctgg ctttttccat cacggcgttt
     2521 tcgtcgcgtc cggcaaagct caggaaaatt tccgagagcg tgacggtttc cggcttggaa
     2581 aacttcacct tgtttttctc gtaataatct ttgatttcct tattgctcag cccgaaatat
     2641 attttcgagt cgacctcgcg ctgcaaaacg taatcgcgcg tgatttgttt gcgccacgat
     2701 tcgcgcagct cttccgggtc gatgccggct ttgcgcattt cgcctttcag cgcgtccagc
     2761 gttttgatgt tctgctcttt cataatttgc agaaaacgct ggttgatgtc gccttcgaca
     2821 tcgatgccga gctccttgcc tttttgcagg ataagctctt cgttgatgat attggcaatc
     2881 aattcgccct ggcggctttc gatttcggct ttcgccactt ccggcgtttt gccctgccgg
     2941 acaagcgcgt cggtcgcgtc ctttatctcg cgtttgatgc gcgaaagcgt cacgacgcct
     3001 tcattgacct gcgccacgac ttcgtcgatg acgaccggct cgctctcctg cgcaaccgcc
     3061 gaaaaaacgg ggaaaaataa aacggcaaca agaaaaattg cggagtttat aaacttcact
     3121 tctctgactc ctttagattt ttgacaaaaa ttcattcaga aatattaatt tcaatagcgg
     3181 attgttgcaa cttatgatgc ccgaaaagcc gtttcggttt cttttaatct tggaaatttc
     3241 actgattttg gaaacttaaa gcgttttgac gttttttata aatagctctt ttctcattcc
     3301 cacgactaat ccgaatgaat aaaaaatgtc cccgatgcaa tttaatcaac tttgccggcg
     3361 ctgaaaaatg caagcgctgc gaatttgatt taa
//
LOCUS       DASWNE010000437         2625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_50447, whole genome shotgun sequence.
ACCESSION   DASWNE010000437 DASWNE010000000
VERSION     DASWNE010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50447"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VGB68_08740"
     CDS             <1..396
                     /locus_tag="VGB68_08740"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY0049356.1"
                     /translation="KADKEGKEHQELFEQIKAELEVHTHIEETIFYPKLKEEKELEDI
                     VLEGIEEHHQAKMFLRELSNLKEDSEKFEPKLKVLMEDITHHVQEEEGEMFPKVEEIF
                     DETTLSELGAQMDAEKKNFQKSSKAGAGK"
     gene            complement(588..1499)
                     /locus_tag="VGB68_08745"
     CDS             complement(588..1499)
                     /locus_tag="VGB68_08745"
                     /inference="COORDINATES: protein motif:HMM:NF020132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HEY0049357.1"
                     /translation="MTKVALTIAGLDPSGGAGIIADIKTFSAFGCFATAAVSSLTFQN
                     TVGVFGTVNQTAESVRGQVEPVLRDFTVSALKTGMLPTREVIEEVARLVRENNLQNFV
                     VDPVVRSTSGFDLIDDAALRALIEKLFPLSSVITPNIAEAERIARIKIENVFDIRRAA
                     RIMQNFGARAVLIKGGHLPFAGAEYESSSAELTSEARSPKNEEQKIEGQKTKDEKRKA
                     IDFMFIGEQEFTFSADFIETDATHGTGCTFAAAITANLANEKTLPEAVETAKRFVTEA
                     IRSAPDLGHGHSPVNHSIANFGLPTAN"
     gene            complement(1610..2299)
                     /locus_tag="VGB68_08750"
     CDS             complement(1610..2299)
                     /locus_tag="VGB68_08750"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEY0049358.1"
                     /translation="MGSHGEKTDLQDISLSAKLIYLITEGEATGENFPEKSARILQIV
                     EAAVRAEISLVQIREKRLPARFVFELTEKAARLTRNSQTKLLVNDRADIAFLAKADGV
                     HLTSLSLSAETIRQNFPRDFIVAVSAHTLAEAESAKRQGADFVTFSPIFRSPNKGEPQ
                     GLIELKKVCAKLRPFPVVALGGIDETNYKSVLEAGASGLAAIRFLNKAENLRSLKPDL
                     SAESTKEIQTG"
     gene            complement(2313..>2625)
                     /locus_tag="VGB68_08755"
     CDS             complement(2313..>2625)
                     /locus_tag="VGB68_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049359.1"
                     /translation="FCFYTHFMVARLTVIFIIFLSLEVGITLTLLPWISFGILGDWGN
                     NYLLAYVAAKTGLPVLQTAVASGWVRGAVTGLGILNIFIAFWEIAHFNESVKMLEGKE
                     K"
BASE COUNT          693 a          622 c          661 g          649 t
ORIGIN      
        1 aaagccgaca aggaaggcaa agagcaccag gaactttttg agcagattaa ggcggaactc
       61 gaagttcaca cgcatatcga ggaaacaatt ttctatccga aactgaaaga agaaaaagaa
      121 ttggaagaca tcgttctcga aggcatcgag gaacatcatc aggcgaaaat gtttctgcgc
      181 gagctttcca atttaaagga agacagcgaa aaattcgagc cgaagctgaa agtcctgatg
      241 gaagacatca cgcatcacgt gcaggaagaa gaaggcgaga tgtttccgaa ggtcgaagaa
      301 attttcgacg aaacgacgct tagcgagctc ggcgcgcaga tggacgccga aaagaaaaat
      361 ttccagaaat caagtaaagc cggcgcgggc aagtaagacg gtttaatgaa tttttaaagg
      421 cgaacggcga gtgaaaaata atttttcact cgccgtttta tttttataaa gcctgatgat
      481 tttcaattac caaagcgtgt tttagatgat taaacgcgct cggaatcgct gatggctgaa
      541 agttgtgtcc gaatccgttt ctgccaaatc aaatttgaaa aatacaatta atttgccgtc
      601 ggcaatccga aattcgcaat cgaatggttg accggcgaat gtccgtgacc taaatcaggc
      661 gcgctgcgaa tggcttcggt gacaaacctt tttgccgtct cgaccgcttc gggcagagtt
      721 ttttcgtttg ccagatttgc cgtaatcgcc gccgcaaacg tgcagccggt tccgtgtgtc
      781 gcgtcggttt cgataaaatc cgcggaaaat gtaaattcct gttcgccgat aaacataaaa
      841 tcgattgctt ttcgcttttc gtccttcgtc ttttgtcctt cgattttttg ttcctcgttc
      901 ttcgggcttc gggcttcgct tgttaattcc gcgctgcttg attcgtattc cgcgcccgca
      961 aacggcaagt gcccgccttt tatcaaaacg gctctcgctc cgaaattctg cattattcgg
     1021 gcggcgcggc gaatgtcaaa aacgttttcg atttttatcc gggcgattct ttccgcttcg
     1081 gcaatattcg gcgtgatgac gctcgaaagc ggaaaaagtt tttcgattaa ggctcgcaaa
     1141 gctgcgtcgt cgattaaatc gaaaccggaa gtcgagcgaa cgaccgggtc gacgacgaaa
     1201 ttctggagat tgttttcccg caccagacgc gcgacttcct cgataacttc gcgcgtcgga
     1261 agcattccgg ttttcaaagc cgaaacggta aaatcccgca aaaccggctc gacctgcccg
     1321 cgtacgcttt ccgccgtttg attaaccgtg ccgaaaacgc cgaccgtgtt ttgaaaagtc
     1381 agagaactga cggcggcggt cgcaaaacag ccgaaagcgg aaaaagtttt aatgtcggcg
     1441 ataatgcccg cgccgcccga agggtcgagc ccggcaatgg ttaaagcaac tttggtcata
     1501 gagatatttt agcgcaaaga tgtgaagaaa aaataattca gccacgagag aaagcaaaga
     1561 agcagcataa aaaagaaagc tcagctttat taattttaaa ttatcctgtc tatccggttt
     1621 gaatttcctt cgtgctctct gcggataaat ccggctttaa gcttcgcaaa ttttccgctt
     1681 tattcagaaa gcgaatcgcc gccaagccgc tcgcgcccgc ttccagaacg gatttgtaat
     1741 ttgtttcatc gatgccgccg agcgcgacga ccggaaacgg tctcagcttc gcgcagactt
     1801 tttttaattc gattaagcct tgcggctcgc ctttattcgg actgcgaaaa atcgggctga
     1861 acgtcacgaa atccgcgcct tgccgtttgg ctgattcggc ttcggcaagc gtgtgcgcgg
     1921 agacggcgac gataaaatct ctcggaaaat tttgtcgaat cgtttcggct gaaagcgaaa
     1981 gggaagtcag gtgaacgccg tcggctttgg cgagaaacgc aatgtcggcg cggtcgttga
     2041 cgagaagttt tgtctgggaa ttgcgcgtca gtcgcgcggc tttttcggtt agttcaaaaa
     2101 cgaatcgcgc gggaagtcgt ttctcgcgga tttgcactaa agagatttcg gcgcggacgg
     2161 cagcctcgac gatttggaga atccgggcgg atttttcggg aaagttttcg ccggtcgctt
     2221 cgccttcggt gatgagatag atgagtttgg ctgaaaggga aatatcttgt aaatcggttt
     2281 tttctccgtg acttcccaac tccgcgactc ctttatttct ctttgccttc gagcattttt
     2341 acgctctcgt taaagtgggc tatttcccag aaggcaataa aaatattcag gattccgagt
     2401 ccggtaacgg cgccgcgcac ccagccggat gcgaccgccg tttgcagaac cggcaagcct
     2461 gttttggcgg cgacataagc cagcagataa ttattgcccc aatcgcccag aatgccgaaa
     2521 cttatccacg gcagcaaagt cagcgtaatg ccgacctcca gactgagaaa aataataaag
     2581 atgaccgtta accgcgcgac cataaaatga gtataaaagc aaaag
//
LOCUS       DASWNE010000438         6282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_367244, whole genome shotgun sequence.
ACCESSION   DASWNE010000438 DASWNE010000000
VERSION     DASWNE010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6282
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367244"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /gene="galK"
                     /locus_tag="VGB68_08760"
     CDS             <1..689
                     /gene="galK"
                     /locus_tag="VGB68_08760"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEY0049360.1"
                     /translation="GIMDQFVSAHARAQHALLLDCRSLEFENVPLDTREVAVVICDTN
                     VKHDLATSGYNTRREECEQAVRILKENLPQVRELRDVSREDFEKYAGFLPELIRRRAR
                     HVVTEIERALRAASALKGKDLTEFGRLMWQSHESLKNDYEVSCRELDVLVEIAMETDG
                     VLGGRMTGGGFGGSTVNLVKRENLDDFKEKIQAEYERLTGIEPTILVSDASEGASEMV
                     FNESSYKKDL"
     gene            712..1773
                     /locus_tag="VGB68_08765"
     CDS             712..1773
                     /locus_tag="VGB68_08765"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY0049361.1"
                     /translation="MSSIKEVAREAGVSTATVSHVINNTRYVSDEVRARVLQAIEQCR
                     YYPNAHARSLASGRSRIIGLVISDIVNPFFPELVKAIEAAAFEHGYDIMLSNTNYDTE
                     RTSHYVRRFIERKIAGVAVMTSEMDKKLVDELAHREVPVVFLDVGEPGLHMNNLRVDY
                     DEGIEQAILHLVALGHRRIAFIGGNTNLRSSRRRLEAFRKTIQQLFPDAPELVFYGNF
                     KIEGGQRAANEILATQGANLPTAVVCANDLMAIGAVSEFEAAGLNVPRDVSVVGFDNI
                     AFAALTKPALTTVNLPLGELGRRAIEVLIRSVEDSEQQGIEIRIPTNLVVRQSTAPAR
                     LQPVDDGASKRCKLVKQTI"
     gene            1787..3433
                     /locus_tag="VGB68_08770"
     CDS             1787..3433
                     /locus_tag="VGB68_08770"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEY0049362.1"
                     /translation="MINKRELALKEKDFSFSRISVYLSAILILLGAAFGAAAQKNATY
                     TNPVLAGDYPDPSVIRVGKDYYATATSSEWAPEFPILHSRDLVNWEIVGTVFPTRPAW
                     SVGNYWAPEIWQENGKFYIFYVARRQNGPLCIAVATASKPTGPYTDHGALECQEVGSI
                     DAFPIRDENGKLFVVWKEDGNSVGKPTPLWAQQLDEKTFKLVGERREILRNDPNTWEG
                     NLVEGPYIMRRNGWFYLFYAGNACCGRECNYATGVARSKTLLGKWEKSPVNPILKGNE
                     SWKCPGHGTIVTDERGRTFLMYHAYDPNDTVYVGRQALLDEVTWSGDDWAVINGGNGP
                     TKQADAPLGIGERNAEYKFFDDFKSRNLRFGWQWLHAIVPNYRLNNGWLVLSPKPEHA
                     RNEIGAMMGWSTTVGNYEATTQLDTRNLSKDAIAGISAFGDNENALGMGVRNGKIIIW
                     KRERNNHQVILTVDAPKSRQLYLQMAALNGHLFRFAVSRDNKKWQVIGGEVDGAYLPP
                     WDRGLRVALVSGGAANASARFGFLRIEPLKTVKAMLGTTP"
     gene            3472..4461
                     /locus_tag="VGB68_08775"
     CDS             3472..4461
                     /locus_tag="VGB68_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0049363.1"
                     /translation="MNFSRAKKVFSLIAILIVALVFAGCERKPANTGGAREGGKKVIG
                     FAQSETDNPWRIAETKSMQDEAAKRADKYEFVLTNAQGQTSKQVSDVEDLIARRVDAI
                     FLVPREAEGLTTALQRAKEAKIPVFLIDREHQGTAGQDFVTFIGSNFVEEGQRVGEWV
                     IKNTGGKAGVVILEGNAGASVARDRNTGFLEAIKSSPDIKVLASQPADFKRANGQAVM
                     ENLIQAHGKNITVVYAHNDEMALGAIQALKTAGMNPGKDVKVVSVDGQKSALEAIIAG
                     DLNVTVECNPRFGPIAFDTLEKHFAGQQIPAKIIVPDKFFDASNAAQFVNEAY"
     gene            4543..6096
                     /locus_tag="VGB68_08780"
     CDS             4543..6096
                     /locus_tag="VGB68_08780"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEY0049364.1"
                     /translation="MNNETILRMEGIRKVFSGVVALDNVDVEFERGKVHSLVGENGAG
                     KSTLIKVMTGAYTRDGGEIFLEGNAVNFRSPEDAQHSGVVAVYQEVNLLMFRSVAENI
                     FLGREPRKFGFVDWSRMSREAGELLQNLGLNINPKAELGSLNIALRQMVAIARGVSIG
                     AKVLVLDEPTSSLTEKEVGILYEVIRRLKAQGTAIVYISHRFDELYAVCDRVTVLRDG
                     KLVGTRDLAGLERIDLVCMMLGKQREELEKKGATAFGEQHEARTESVPLLKTENLNRG
                     RRLKNVSLEARHGEILGMAGLLGSGRTDVARAVFGADKVEGGKVSLNGKELNLNSPTD
                     AINAGIAFLSEDRKAEGIIPEWSVRENLTLAALPALTTMGVVSRARQNEIVDKFIKRL
                     GIKTSSADQKIRELSGGNQQKVLLARWLCKSPKFMIMDEPTRGIDIGAKGEIQKLVNE
                     LANEGLGVLMISSELEELVEGSSRVVVMRDGELVAELRRDQISQDAIINAMAEADDET
                     AANGETASV"
     gene            6074..>6282
                     /locus_tag="VGB68_08785"
     CDS             6074..>6282
                     /locus_tag="VGB68_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049365.1"
                     /translation="MEKLQVFNFSRKLVALIFVAFLCVSGVVAQERWTEQKAKDWYAA
                     EGWLVGANFVPASAINELEMWQADT"
BASE COUNT         1553 a         1551 c         1888 g         1290 t
ORIGIN      
        1 tcgggattat ggaccagttc gtttcggctc acgcccgcgc gcagcacgcg cttctgctcg
       61 attgccgtag tctggaattt gaaaacgtcc cgctcgacac gcgtgaagtc gcggtcgtaa
      121 tttgcgatac gaacgtaaaa cacgatttgg cgacatcggg ctataatacg cggcgcgaag
      181 aatgcgagca agccgtgcga atattgaaag aaaatttgcc gcaggttcgg gaattaagag
      241 acgtcagccg cgaggatttt gaaaaatacg caggcttttt gcccgaattg attcgtcgcc
      301 gagcgcgcca cgtcgttacg gaaatcgagc gcgctctgcg agccgcaagc gctttaaagg
      361 gcaaagactt gaccgaattc gggcgcttga tgtggcagtc gcacgaaagc ctgaaaaacg
      421 attatgaggt aagctgtcgg gaattggacg tgctggtcga aattgccatg gaaacggacg
      481 gcgttctcgg cgggcgaatg acgggcggcg gtttcggcgg ctcgaccgtg aatctggtga
      541 aaagagaaaa tctcgacgat tttaaagaga aaatacaggc ggaatacgag cgattgaccg
      601 gaatcgagcc gacgatttta gtatcggatg cgagcgaggg ggcgagtgaa atggtattta
      661 atgaatcgag ttacaagaaa gatttgtaac tgaaaaaaaa gtttaataat tatgagcagc
      721 atcaaggaag tggcacgcga agcaggcgtt tcgaccgcga cggtttcgca cgttatcaac
      781 aatacgcgct acgtctcgga cgaagttcgc gcccgcgtgc tgcaagccat cgaacaatgc
      841 cgctattacc cgaacgcgca cgcgcgcagc ctcgcttcag gcagaagcag gataatcggg
      901 ctggttattt ccgacatcgt caacccgttt ttccccgaac tggtgaaagc catcgaagcg
      961 gcggcgttcg agcacggcta cgacattatg ctgtcgaata cgaattacga caccgagcgg
     1021 acttcgcatt acgtgcggcg gtttatcgag cgcaaaatcg cgggcgtcgc ggtaatgact
     1081 tcggaaatgg acaaaaagct ggttgacgag ctggcgcatc gcgaagttcc ggtggttttt
     1141 ctggatgtcg gcgagccggg cttgcatatg aacaatctgc gcgtcgatta tgacgaaggc
     1201 atcgagcagg cgattctgca tctggtcgcg ctcggtcacc ggcgcatcgc ttttatcggc
     1261 ggaaatacta acctgcgttc ttcgcggcgg cggctcgaag cttttcggaa gacgatacag
     1321 cagctttttc cggacgcgcc ggagctggtt ttttacggca atttcaaaat cgagggcggt
     1381 cagcgcgcgg cgaacgaaat cctggcgacg caaggcgcga atctgccgac ggcggtcgtc
     1441 tgcgctaacg atttgatggc aatcggcgcc gtcagcgagt ttgaagcggc gggcttaaac
     1501 gttccgcgcg acgtttccgt tgtcggtttc gacaacatcg cttttgccgc gctgaccaaa
     1561 ccggcgctga cgaccgtcaa tctgccgctg ggcgagctcg gacggcgcgc catcgaggtt
     1621 ttgattcgca gcgtggaaga ttccgagcag cagggaatcg agattcgcat tccgaccaat
     1681 ctggttgtcc ggcaatcgac cgcgcccgcg cgcctgcagc ccgtcgatga cggcgcgagc
     1741 aaaagatgta aattggtcaa acaaactatt taagaggaga aaatttgtga ttaacaagcg
     1801 ggaactggca ttaaaagaaa aagatttttc gttttcccgg ataagcgttt atttgtcggc
     1861 aattttaatt ttgctcggcg cggcgttcgg agcggcggcg caaaaaaacg cgacttacac
     1921 caatccggta ctggcgggcg attatcccga cccttcggtg attcgcgtcg gcaaggatta
     1981 ttacgcgacg gcgacttcga gcgaatgggc gccggaattt ccgattctgc attcgcgcga
     2041 tttggtcaac tgggaaatcg tcggcacggt ttttccgacg cgtccggcgt ggtcggtcgg
     2101 aaattactgg gcgccggaaa tctggcagga aaacgggaaa ttttacatct tttacgttgc
     2161 ccggcggcag aacggaccgc tttgcatcgc ggtggcgacg gcgtcaaaac cgaccggacc
     2221 ttacaccgac cacggcgcgc tggaatgcca ggaagtcggc tcgatcgacg cgtttccgat
     2281 tcgcgacgaa aacggaaaac tttttgtcgt ctggaaagaa gacggcaaca gcgtcggcaa
     2341 accgacgccg ctctgggcgc agcaattgga cgaaaaaact ttcaagctcg tcggcgagcg
     2401 ccgcgaaatt ttgcgcaacg acccgaacac ttgggaaggc aatctggtcg aaggtccata
     2461 tataatgcgc cgcaacggct ggttttacct tttctatgcg ggcaacgcct gctgcgggcg
     2521 cgagtgcaat tacgcgacgg gcgtggcgcg ctcgaaaacg cttctgggaa aatgggaaaa
     2581 aagtccggtg aatccgattt tgaaaggcaa tgaaagctgg aaatgtcccg gtcacggcac
     2641 gattgtgacg gacgaacgcg ggcgaacttt cctgatgtat cacgcctacg acccgaacga
     2701 tacggtctat gtgggcaggc aagcgctgct cgacgaagtt acctggtcgg gcgacgattg
     2761 ggcggtgata aacggcggaa acggtccgac caaacaggca gacgcgcctc tcggcatcgg
     2821 cgaacgcaac gccgaataca aatttttcga tgatttcaaa tcgcgaaact tgcgtttcgg
     2881 ctggcaatgg ctgcacgcga ttgtgccgaa ttacagatta aataacggct ggctggtttt
     2941 atcgccgaag ccggaacacg cgcgaaatga aatcggcgcg atgatgggct ggtcgacgac
     3001 ggtcgggaat tacgaggcga cgacgcagct cgatacgaga aatctgtcga aagatgccat
     3061 tgccggaatt tccgctttcg gcgacaacga aaacgcgctc ggaatgggcg tccgcaacgg
     3121 taaaatcatt atttggaaac gcgagcgaaa caatcatcaa gtcattttga ctgtcgacgc
     3181 gccgaaatcc agacagcttt acctgcaaat ggcggcttta aacgggcatt tattccgctt
     3241 tgccgtcagc cgcgacaata aaaaatggca agtgatcggc ggggaagtgg acggcgctta
     3301 cctgccgccg tgggacagag gcttgcgcgt cgcgctcgtt tcgggcgggg cggctaatgc
     3361 gtcggcgcgg ttcggatttt tgcgaatcga gccgcttaaa accgtaaaag ctatgctcgg
     3421 tacgacgcct tagccagccg ggaaaaaaat taaacaatcg ggagcaaata aatgaatttt
     3481 tcaagagcaa aaaaggtatt cagtttaatc gcaattttaa tcgtcgcgct tgttttcgca
     3541 ggctgcgagc gcaaaccggc aaatacgggc ggcgcgcgcg aaggcggcaa aaaggtaatc
     3601 ggtttcgcgc aatcggaaac cgacaatccg tggcgcatcg ccgaaacgaa atcgatgcag
     3661 gacgaagccg ccaagcgggc ggataaatac gagttcgtcc tgacgaacgc gcaggggcag
     3721 acttccaaac aggtttccga cgtggaagat ttaatcgccc gccgcgtcga tgcgattttt
     3781 ctcgttccgc gcgaagccga aggcttgacg accgccttgc agcgagcgaa agaagcgaaa
     3841 attccggttt ttctgattga ccgcgagcat cagggaacag ccggacagga tttcgtcacc
     3901 tttatcggct cgaattttgt cgaggaaggt cagcgcgtcg gcgaatgggt cattaaaaat
     3961 acgggcggca aagccggagt cgtcattctc gaaggcaacg ccggggcaag cgtcgcgcgc
     4021 gaccgcaaca cgggttttct ggaagcgatt aaatccagcc cggacataaa agttctggct
     4081 tcgcagcccg ccgatttcaa acgcgccaac gggcaggcgg taatggaaaa tctgattcag
     4141 gcgcacggca aaaacatcac ggtcgtctac gcgcacaacg acgaaatggc gctcggcgcg
     4201 attcaggctc tgaaaaccgc cggaatgaat cccggcaaag acgtcaaggt cgtctcggtc
     4261 gacggtcaga aatcggcgct cgaagctatc atcgcgggcg atttaaacgt gacggtcgaa
     4321 tgcaacccgc gtttcggtcc gattgctttc gacacgctcg aaaaacattt tgcaggacaa
     4381 cagattccgg caaaaattat cgtgcccgac aagtttttcg acgcgagcaa cgcggcgcaa
     4441 ttcgtcaacg aagcttatta actgaaaact gataactgat agcggatagc gaaagcgaaa
     4501 caattatcgg ttatcggtta ccaactacca gttataaaaa ctatgaacaa cgaaacgatt
     4561 ttgcgaatgg aaggcatcag gaaagttttt tcgggcgtgg tcgcgctcga taacgtcgat
     4621 gtcgagtttg aaagaggcaa ggttcactcg ctggtcggcg aaaacggcgc gggcaaatcg
     4681 acgctgatta aggtgatgac gggcgcgtat acgcgcgacg gcggcgagat ttttctggaa
     4741 ggcaatgcgg taaattttcg ctcgcccgaa gacgcacagc attcgggcgt cgtcgccgtc
     4801 tatcaggaag tcaacctgct gatgtttcgc agcgtcgcgg aaaatatctt tttaggtcgc
     4861 gagccgcgca aattcggctt tgtcgattgg tcgcggatga gccgggaagc gggcgagctt
     4921 ttgcaaaatc tcggattgaa tatcaatccg aaagccgagc ttggaagcct gaatatcgct
     4981 ctgcggcaga tggtcgccat cgctcgcggc gtttccatcg gcgcgaaagt tctggtgctg
     5041 gacgagccga ccagctcgct gacggaaaag gaagtcggga ttttgtatga agtgattcgg
     5101 cgcttgaagg cgcagggcac ggcgattgtt tacatctcgc accgctttga cgagctttac
     5161 gcggtctgcg acagggtgac ggttttgcgc gacgggaaac tcgtcggaac gcgcgatttg
     5221 gcggggctcg aacgcatcga cctggtttgt atgatgctcg gcaaacagcg cgaggagctg
     5281 gaaaagaaag gcgcgaccgc tttcggcgaa cagcacgagg cgcggacgga atcggtgcct
     5341 ttgctgaaaa ccgaaaacct gaatcgcggg cggcgtctga aaaacgtctc gctcgaagcc
     5401 cgtcacggcg aaattttagg aatggctgga cttttaggtt cgggaagaac ggatgtggcg
     5461 cgcgcggttt tcggcgccga caaggtagaa ggcggaaaag tttcgctcaa cggaaaagag
     5521 cttaatctca attcgccgac cgacgccatc aatgccggaa tcgctttttt gagcgaagac
     5581 cgcaaagccg aaggcatcat tcccgaatgg tctgtccgcg aaaatctgac gctggcggct
     5641 ttgcccgctt tgacgacgat gggcgtcgtc tcgcgcgcca gacaaaatga gattgtcgat
     5701 aaatttataa agcgtctcgg catcaagacg tcgagcgccg atcagaaaat cagggaatta
     5761 agcggcggaa atcagcagaa agttctgctg gcgcgctggc tctgcaaaag tccgaaattt
     5821 atgattatgg acgagccgac gcgcggcatt gacatcggcg cgaaaggcga aattcaaaag
     5881 ctcgtcaatg aattggcaaa cgaaggcttg ggcgttttga tgatttcgtc ggaactggaa
     5941 gagctcgtcg aaggcagctc gcgggtcgtc gtgatgcgcg acggcgagtt ggtcgcggaa
     6001 ttgcgccgcg accagatttc gcaggacgcg attatcaacg cgatggcgga agccgacgac
     6061 gaaacggctg caaatggaga aactgcaagt gtttaatttt tcgagaaaat tagtcgcact
     6121 cattttcgtc gcgtttttgt gcgtttccgg cgttgtcgcg caggaacgct ggacggagca
     6181 aaaagcaaaa gactggtatg ccgccgaggg ctggctggtc ggcgcgaatt ttgttccggc
     6241 aagcgcgata aacgagctgg aaatgtggca ggcagacacc tt
//
LOCUS       DASWNE010000439         1785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_120106, whole genome shotgun sequence.
ACCESSION   DASWNE010000439 DASWNE010000000
VERSION     DASWNE010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120106"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..267)
                     /locus_tag="VGB68_08790"
     CDS             complement(1..267)
                     /locus_tag="VGB68_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049366.1"
                     /translation="MSKLKSYLVAAVLFAFLLAAWALANNFPTAQAQSLSDKQWETSV
                     VRGGSLPEGLQGVLNTRGEEGWELVDVVQSKDGNYVAFLKRRKR"
     gene            complement(583..1440)
                     /locus_tag="VGB68_08795"
     CDS             complement(583..1440)
                     /locus_tag="VGB68_08795"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0049367.1"
                     /translation="MVTGFVKAGAATLNYIVEGTGSPVLIIGSANYYSRTFSGRLRES
                     LKMAFTDLRHFAEQSSVPSFARLTFEICTGDIEQVRSALGFEKFMIIGHSHHGNIALD
                     YAKRFPERVSHVVIIGSPPVNVQRTIEAAEAFWTEYASEKRKSALRRNRKALEANISG
                     AAMSPAEAFIAEYVADGPKYWYDTDYDASWLWRDVPVNMDIIRLFRDLFADYELTWNA
                     ENLKAPVLVVMGRHDYVVPHLLWQHVLSKLKNVTFHLFEQSGHTPQLEEPELFDRILL
                     EWLGKEGAA"
     gene            1594..1782
                     /locus_tag="VGB68_08800"
     CDS             1594..1782
                     /locus_tag="VGB68_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049368.1"
                     /translation="MGKVYAGTTDGRRLIANIQFSFYPIKKMTRLKNRAIPRIALQNL
                     QIPNSGGQFQNVSDKPGN"
BASE COUNT          478 a          428 c          401 g          478 t
ORIGIN      
        1 ttatcgcttg cggcgtttga gaaaggcgac gtaatttcca tctttggact gcaccacgtc
       61 aaccagctcc caaccttcct cgcctctggt attgaggacg ccctgcaagc cttccgggag
      121 cgacccgccc cgcacaaccg acgtttccca ctgcttatcg gataaactct gcgcctgcgc
      181 cgtcggaaaa ttgttcgcga gcgcccacgc tgcgagtaaa aaggcaaata aaactgccgc
      241 gaccagataa ctttttaatt tgctcataaa tataatctcc gcttttttat ttaatttaag
      301 gctcgctaaa agcccgacaa aaagcagtct aaatcctgta gtcgaataca gagtcaagag
      361 atattttgaa ttttttaggc gggataaaag atttttcttt cgctttgatt caataaaaaa
      421 tatcttgagc aggcagacaa aggttttctc aagtttcagc tcatcaggac attgatcagg
      481 aaactctata atcggatttt ttgatttttt taatccggaa gaatagaaag agagtgaatc
      541 gggcgattaa ttttatttta tcgccggctg acattctttt tttcaggcag ccccttcctt
      601 tccaagccat tcgagcaata tgcggtcaaa caactccggt tcttcgagct gcggagtgtg
      661 cccgctttgt tcaaataagt gaaaggttac attctttagc tttgacagca cgtgctgcca
      721 gagaagatgc ggaacgacat aatcatgccg tcccatgaca accagaaccg gagcttttaa
      781 attctccgca ttccaggtca attcgtagtc cgcgaacaaa tcgcggaaaa ggcgaataat
      841 atccatattg accggaacat ctcgccataa ccaggaagcg tcgtagtccg tgtcatacca
      901 gtattttggt ccgtcggcga catactcggc gataaaagct tcggcgggcg acattgctgc
      961 gccggaaata tttgcttcaa gcgctttccg gttacgccgt agggcggatt tccgcttctc
     1021 cgaggcatat tccgtccaga atgcctcagc cgcctcgatt gttcgctgca cattaaccgg
     1081 aggagatccg atgatgacga cgtgagacac tctttctgga aatcgtttgg cgtaatccaa
     1141 ggcgatatta ccgtggtgcg aatgcccgat aatcataaat ttttcaaatc cgagcgcaga
     1201 ccgcacctgt tctatatcgc cggtgcagat ttcaaatgtg agcctcgcga aagaaggtac
     1261 cgaactttgt tccgcaaaat gacgcagatc cgtgaatgcc attttaaggc tctctctgag
     1321 cctcccggag aatgttctcg aataataatt tgccgagcca atgatgagga cggggctgcc
     1381 ggtcccttca acgatgtagt tgagagttgc cgcgccggct ttcacaaatc ctgtgaccat
     1441 aataatttcg cgagctggga ttcatatcgg ggcaggaaaa acttgctggc gcgcaatgcc
     1501 gtactttttc cttttgattt tcgtcccgct ttccgcctgt cataaataat aaacgatgta
     1561 gccgaataca gggtcaagtg gtttttgaaa aatatgggaa aagtttatgc agggacaaca
     1621 gacggtcgcc gtctgattgc aaacatacag tttagttttt atccgataaa gaaaatgacg
     1681 cgattgaaaa atcgcgccat tcctcgtata gctttacaga atttacaaat cccgaattca
     1741 ggcgggcagt ttcaaaatgt atctgataag cccggcaact aaaat
//
LOCUS       DASWNE010000440         2480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_226484, whole genome shotgun sequence.
ACCESSION   DASWNE010000440 DASWNE010000000
VERSION     DASWNE010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226484"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="VGB68_08805"
     CDS             <1..869
                     /locus_tag="VGB68_08805"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0049369.1"
                     /translation="KMKRLNFVWLLLILCFAASSAPAQTEKRALKSKFAAFGGVKVHY
                     NDVGKGKNALVFVHGWTCSADFWRQSVDEFPNYRVVALDLPGHGMSDKPRADYSMEYF
                     AKSVEAVLKDAGIKKAVLVGHSMGTPVIRQFYRLYPEKTLGLIIVDGALRPMGTKQQE
                     QQYLAFLRADYKANAPKMVDGMLQPITDEKLRREIRSAMLATPEHVAVSAMAGMTDEK
                     IWANDKIDVPVLAILAQSPFWKPDTADFYRRLAPNLDFRMWQGVSHFLMMERPQVFNQ
                     SVRFFVEKNKLL"
     gene            980..1507
                     /locus_tag="VGB68_08810"
     CDS             980..1507
                     /locus_tag="VGB68_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049370.1"
                     /translation="MQRDASKTIVAALAAIHFLWCAYDPSKAVFPHLVHTPIHEAGHV
                     LFRLFGEFMGIAGGSLFQVAVPLVFFGYFLYHRKPFSASIVLFWVGQSITDVYVYAGD
                     AAAMQLPLLSGLTGAEGGFHDWNYLLTETGLLGFTPVIAKIIRFTGTLIIFAAIIGSF
                     LYAVKGEKILTDTDL"
     gene            1504..2295
                     /locus_tag="VGB68_08815"
     CDS             1504..2295
                     /locus_tag="VGB68_08815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0049371.1"
                     /translation="MKATLITGASGGIGEAFARRLAREKHNLVLVARSEKKLHELCDE
                     LMLRHQITAHYVTIDLTEFEADRRLFEETERHDFEIDRLVNNAGFGSMGDFAKLPLEN
                     EMQMISLNITALVALTHRYLAKMRERRSGTIINVSSSAGFQPIPFMATYAATKAFVTS
                     FSEALAEENRPFGITVTALCPGPTDTNFFAVANAKPLQMKGMQSPEEVVETALDAARR
                     GRASVVSGWTNYIGSFLGTFVPNAVVTRAIGGVLRPQVEKNSTKK"
BASE COUNT          583 a          628 c          663 g          606 t
ORIGIN      
        1 ccaaaatgaa aagattaaat ttcgtctggc ttttgctgat tttgtgtttc gccgcttctt
       61 ccgctccggc gcagacggaa aagcgagctt taaaaagtaa attcgccgcg ttcggcggcg
      121 tgaaagttca ttacaacgac gtcggcaaag gcaaaaacgc gctcgtcttc gttcacggct
      181 ggacgtgcag cgccgatttc tggcggcaga gcgtcgacga atttccgaat tatcgcgtcg
      241 tcgccctcga tttgcccgga cacgggatga gcgacaagcc gcgagccgat tattcgatgg
      301 agtatttcgc caaatccgtc gaagccgtgc tgaaagacgc cggaatcaaa aaagccgtgc
      361 tggtcggtca cagtatggga acgccggtta ttcgccagtt ctaccgtctc tatccggaaa
      421 aaactctggg cttgataatt gtcgacggcg cgctgcgacc gatgggaaca aagcagcagg
      481 agcagcaata tttggctttt ctgcgcgccg attacaaggc gaacgcgccg aaaatggtcg
      541 acggaatgct ccagccgatt accgacgaaa agctccgacg ggaaattcgc tcggcgatgc
      601 tcgccacgcc cgaacacgtc gccgtcagcg cgatggcggg aatgaccgac gaaaaaatct
      661 gggcgaatga caaaatcgac gttcccgtgc tggcgatttt ggctcagtcg cctttctgga
      721 aaccggacac ggcggatttt tatcgccggc tggcgccgaa tctggatttt cggatgtggc
      781 agggcgtcag ccattttctg atgatggagc gcccgcaggt ttttaatcaa tcggtgcggt
      841 ttttcgtcga gaaaaataaa ttgctttaaa aacaagacgc cgattgttta agatagatta
      901 ggattttatt ttgatttatc ctaaataatc ccaatctatc ttaaacaagc ctaaaaaatc
      961 ggcgttcaat tcctgccaaa tgcaaagaga cgcttccaaa acaatcgtcg ccgcccttgc
     1021 cgcaattcat tttctctggt gcgcttacga cccgtcgaaa gccgtttttc cgcatctcgt
     1081 gcacacgccg attcacgaag cgggacacgt tttatttcgc cttttcggcg agtttatggg
     1141 cattgccggc ggctcgctgt ttcaggtcgc cgtgccgctc gtctttttcg gttatttcct
     1201 ttaccaccgg aaaccttttt cggcttcgat tgttttattc tgggtcgggc agagcatcac
     1261 ggacgtatac gtttacgcgg gcgacgcggc ggcgatgcag cttcctttat tgagcggttt
     1321 gaccggcgcg gaaggcggtt ttcacgattg gaattattta ttgaccgaaa ccggcttgct
     1381 cggatttacg ccggttatcg caaaaataat tcgttttacg ggaaccctga taatttttgc
     1441 ggcaattatc ggctcgtttt tgtatgctgt taaaggcgaa aaaattttaa cggatacgga
     1501 tttatgaaag cgacattgat aacgggcgcg tccggcggca tcggcgaagc gtttgcgcgg
     1561 cggctggcgc gggaaaaaca caatctggtg ctcgtcgcgc gctccgaaaa aaaacttcac
     1621 gagctgtgcg acgaattgat gcttcggcac caaatcacgg cgcattacgt caccatcgat
     1681 ttgacggaat ttgaagctga ccggcggctt tttgaagaaa ccgagcggca cgatttcgag
     1741 attgaccggc tggtcaacaa cgccggtttc ggctcgatgg gcgattttgc gaagctgccg
     1801 ctcgaaaacg agatgcaaat gattagcctc aacataacgg cgctggtcgc cctgacgcat
     1861 cgctacctgg cgaaaatgcg cgagcgccgg agcggaacga ttatcaacgt ttcctcttcg
     1921 gcgggttttc agccgattcc gtttatggcg acttacgcgg cgaccaaagc cttcgtcacg
     1981 tctttttccg aagcgctcgc cgaagaaaat cgcccgtttg gcatcaccgt cacggcgctc
     2041 tgcccgggac cgacggacac aaattttttc gccgtcgcca atgcgaaacc gctccagatg
     2101 aaaggaatgc agtcgccgga agaagtcgtc gaaacggctt tagacgcggc gcggcgcggg
     2161 cgcgcaagcg tggtttccgg ctggacgaat tatatcggct cgtttctcgg aacgtttgtg
     2221 ccgaatgcgg tcgtgacgcg cgccatcggc ggcgttctgc gaccgcaggt cgagaaaaat
     2281 tcgacgaaaa aataaacgca aaaacgacga gcggcaagaa caagaaaaga tttgcccgcc
     2341 aaacaaacga aaatcgcgaa aagaagaaaa caataattgg ttttcttttt ccttattttt
     2401 gtttgtttcg cttggtttcg cgggtaaatc ttctttcgcg tccttgccag tttacggctt
     2461 cgcgttaaat aattttatta
//
LOCUS       DASWNE010000441         3104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_473598, whole genome shotgun sequence.
ACCESSION   DASWNE010000441 DASWNE010000000
VERSION     DASWNE010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473598"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..782)
                     /locus_tag="VGB68_08820"
     CDS             complement(102..782)
                     /locus_tag="VGB68_08820"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEY0049372.1"
                     /translation="MGDAERRDSSQLIESSLRVSASPRPRVPTIGLDFETRAIADGFR
                     FIAGVDEVGRGCLAGAVVAAAVILDLSKPIPAGLNDSKKLTAKKRETLDEEIRQNAVA
                     FSIAQVEADELDRINILQATKKAMRLAIENLDPKADFLLIDALELKDLELPQKAIIHG
                     DAISASIAAASIIAKTFRDNLMQEMDKLYPAYGFAKHVGYGTKAHFEALKMHGACPLH
                     RKSFRGVV"
     gene            complement(805..1182)
                     /gene="rplS"
                     /locus_tag="VGB68_08825"
     CDS             complement(805..1182)
                     /gene="rplS"
                     /locus_tag="VGB68_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEY0049373.1"
                     /translation="MNRLDFIENTQLKENIPAFQPGDTVRVHVRIKEGNKERLQAFEG
                     MCIARKHGGARETVTVRKVSFGVGVERIFPLHATIVDRIDVVRRGKVRRAKLYYLREL
                     RGKAARIKERDTRNKDAANAAKK"
     gene            complement(1253..1960)
                     /gene="trmD"
                     /locus_tag="VGB68_08830"
     CDS             complement(1253..1960)
                     /gene="trmD"
                     /locus_tag="VGB68_08830"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEY0049374.1"
                     /translation="MKIDVLTIFPEFFREVFDFGIIRRARLAEIVEINVHDLRSFATD
                     KHKMVDDRPFGGGDGMVFKPEPVFNAIENLIGTCERENYPPQTRVVLLTPQGRVLKQT
                     VAREFADQAAHVVLICGRYEGVDERVNETLVTDEISIGDYVLSGGEPAAIVLVDAITR
                     LLPNALGSETSAVNDSFTENLLDCPHYTRPAEFRGRRVPEVLLNGNHAEIEKWRKQKA
                     LEKTRKNRKDLLSKENS"
     gene            complement(2039..2557)
                     /gene="rimM"
                     /locus_tag="VGB68_08835"
     CDS             complement(2039..2557)
                     /gene="rimM"
                     /locus_tag="VGB68_08835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEY0049375.1"
                     /translation="MEEFVAIARTVRTRGLRGELVAEVLTDFPERFEETETVFAIKPD
                     GGREQLKLENHWFQKNRVILKFAGFDSVEAAESLINCEICIPESEAVELEEGEFFDWE
                     LIDCAVETVEGETLGRVREVMRTGGTEILVVVSAKQEKDYLIPFAEKICVEVDIENKL
                     IRVDAPEGLLEF"
     gene            complement(2682..2918)
                     /locus_tag="VGB68_08840"
     CDS             complement(2682..2918)
                     /locus_tag="VGB68_08840"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEY0049376.1"
                     /translation="MKEAVEKLVKALVGNADAVEVSEKPGDSANTTVISVKVGAGEMG
                     RLIGREGRTIKAIRSLLYAASQKQRKRFVLDVIE"
BASE COUNT          677 a          841 c          756 g          830 t
ORIGIN      
        1 accttaaata gagttttatc aaccttaaat agagttttat gaattgtaaa ttgagtttta
       61 tcaaccttaa atagagtttg acgggcgtta aatctgactt ttcaaaccac gccgcgaaag
      121 cttttgcggt gcagcgggca cgcgccgtgc atttttaaag cttcgaagtg agcttttgtt
      181 ccgtagccga cgtgttttgc aaagccgtac gcgggataga gcttgtccat ttcctgcatt
      241 aaattatcac gaaaggtctt ggcgataatg gaagcggcgg cgatggaagc ggaaatggcg
      301 tcgccgtgaa taatggcttt ttgcggaagt tcgaggtctt tcagctcaag ggcgtcaatc
      361 agcagaaaat cggctttcgg gtcgaggttt tcgattgcca ggcgcatcgc ttttttcgtc
      421 gcctgcagga tgttgatgcg gtcgagttcg tccgcttcga cctgtgcaat cgagaaagcg
      481 acggcgtttt gtcttatttc ctcgtcgagc gtttcgcgct ttttcgcagt gagttttttg
      541 gaatcgttca gccccgccgg aatcggcttc gacaaatcca gaatcacggc ggcggcgacc
      601 accgcgcccg ccagacaacc gcgcccgacc tcgtcgacac cggcgataaa gcgaaacccg
      661 tcggcaatcg cgcgggtttc aaaatccaga ccgatggtcg ggacgcgcgg acgcggagac
      721 gcggagacgc ggagagagga ttcaatcagc tgcgacgaat ctcttctttc cgcgtctccc
      781 attcttcgcc tctccccgtg tcgcctattt tttcgcggcg ttggcagcgt ctttgttgcg
      841 tgtatcgcgc tctttgatac gagcggcttt gccgcgcagc tcgcgcagat aataaagttt
      901 ggcgcggcgc accttgccgc ggcgcacgac gtcgatgcgg tcgacaatcg tcgcgtgaag
      961 cggaaaaatt ctttccacgc cgacgccgaa gctaaccttg cgaaccgtca cggtttcgcg
     1021 cgcgccgccg tgtttacggg cgatgcacat accttcgaaa gcctgcaggc gctctttgtt
     1081 tccttccttg atgcgaacgt gaacgcgaac ggtatcgccc ggctgaaaag ccggaatatt
     1141 ttcttttaac tgcgtatttt caataaaatc caagcggttc atttttttta tcagtgagag
     1201 gtgaaacgcg cgaagtaacc gtctaattcc cggatacctt ttgctactca cttcaactat
     1261 tctccttact taataaatct tttctattct tccgcgtttt ttccaacgct ttctgtttac
     1321 gccatttttc aatctcggcg tgatttccgt ttaataaaac ttccggcact cgtcttccgc
     1381 gaaactccgc cggtcgcgtg taatgcgggc agtccagcaa attttccgta aacgaatcgt
     1441 tgacggctga cgtttcgctg cccaaagcgt tcggtaaaag ccgcgtaatc gcgtcgacca
     1501 gaacaatcgc cgccggttcg ccgcccgaaa ggacgtaatc gccgatggaa atttcgtcgg
     1561 tgactaaagt ttcgttgact ctttcgtcga cgccctcgta gcgaccgcaa atcaaaacga
     1621 cgtgcgcggc ttggtcggca aattcgcgcg cgaccgtctg ttttaaaact ctgccctgcg
     1681 gcgtcaataa aacaacgcgc gtttgcggcg gataattttc gcgctcgcac gttccaatca
     1741 ggttttcaat cgcgttgaaa accggttcgg gcttgaaaac catcccgtcg ccgccgccga
     1801 acggtcggtc gtcgaccatt ttgtgcttgt cggtcgcgaa agagcgcaaa tcgtgaacgt
     1861 ttatctcgac gatttccgca agcctggcgc ggcggattat tccaaagtca aagacctctc
     1921 gaaaaaattc agggaaaatt gttaaaacgt caattttcat ttgttctcag gtcttaagga
     1981 cttgggtctt tgcttttgta tttacgaaga ccaaagaccg aaaaccgaaa accggcaatc
     2041 aaaattccaa aagtccttcg ggcgcatcta ctcgaatcag cttgttttca atatcgactt
     2101 cgacgcaaat cttttcggca aacggaatca gataatcttt ttcctgtttc gcgctcacga
     2161 cgacgagaat ttccgtgccg cccgtgcgca tcacttcgcg cacgcgcccg agcgtttcgc
     2221 cctcgacggt ttcgaccgcg cagtcaatca gttcccaatc gaaaaactcg ccttcttcca
     2281 gctcgaccgc ctcggattcg ggaatgcaga tttcgcagtt tatcaagctt tccgccgctt
     2341 cgacggaatc aaacccggcg aactttaaaa tcacgcggtt tttttgaaac caatgatttt
     2401 cgagctttaa ctgctcgcgc ccgccgtccg gcttaatcgc aaaaaccgtt tccgtttctt
     2461 cgaagcgttc cggaaaatcg gttaaaacct cggcaaccag ctcgccgcgc agcccgcgcg
     2521 ttctgacggt tctggcgatg gcaacgaatt cttccattcg agtttgctga tagttgcaga
     2581 cagttttcag gtagttgcag gtagttatcg aagattgaag ctttgtaatt gtttttaact
     2641 atctgcaact atccgcaact ttctccaaac tttctgcaaa cttattcgat tacatccagc
     2701 acgaaacgct tgcgctgttt ctggctggcg gcatacagaa ggcttcgaat cgctttgatc
     2761 gtccgacctt cgcgcccgat aaggcgaccc atttcgcccg cgccgacctt cacggaaata
     2821 acggtcgtgt tggcgctgtc gcccggtttt tccgacactt cgaccgcatc ggcgttgccg
     2881 accagggctt tgaccagttt ttcgacggct tctttcataa gtttaccggg ttgactgaaa
     2941 gttaactgag ttgaccgagt taaagaaagt ttttaactcg gtcaactcag ttaactcgct
     3001 aaactgacaa ctattcttcc gctgctgctg ccggattgtt tttaatcagg ctcgccacgg
     3061 tttcggtcgg ctgcgcgccg acgctaatcc agtattgaat gcgg
//
LOCUS       DASWNE010000442         7680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_84314, whole genome shotgun sequence.
ACCESSION   DASWNE010000442 DASWNE010000000
VERSION     DASWNE010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7680
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84314"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VGB68_08845"
     CDS             complement(<1..297)
                     /locus_tag="VGB68_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049377.1"
                     /translation="MTDYRYITINTLHARNRDDYTNERDICTRHRKDYTLDRAKYTEM
                     RGVYTGNRTNYTPDRSKDTVNRRDYTVDRGKHTSDRGSYTLDRTLHRDDRRIHTS"
     gene            complement(346..786)
                     /locus_tag="VGB68_08850"
     CDS             complement(346..786)
                     /locus_tag="VGB68_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049378.1"
                     /translation="MDKIKAKYLATGDRVRFSRDGWQTVGRITNCNSISVSFMFGDGV
                     IRKLEAHQEYVVEKAEKSPEPQPGQIMRVNKKSKRISVCASHSLFDLSDISESFRAIK
                     QRGSYSNSAAAYYEPERGATLHTGDSVYWMPLKWSGEPTKETGS"
     gene            1132..1413
                     /locus_tag="VGB68_08855"
     CDS             1132..1413
                     /locus_tag="VGB68_08855"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0049379.1"
                     /translation="MGRASLEKPERLAEKLCQIRNALNLSQNELIRWLDLTDKLTQAE
                     ISAFERGVRIPPLLVLLRYARKFNINVEILIDDELDLPEKLPNKLVNNR"
     gene            complement(2096..2515)
                     /gene="tnpA"
                     /locus_tag="VGB68_08860"
     CDS             complement(2096..2515)
                     /gene="tnpA"
                     /locus_tag="VGB68_08860"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEY0049380.1"
                     /translation="MSRRAYSEINFHITWHTKSNLPSIKPELEPKLYQFLKDRILETP
                     ETIFHAAGGIENHIHIAVSVPPTIQPAEWIGQLKGSSSHYINQLANRKLLEWQHGYGI
                     VSFGTKDLKWVVNYVLNQKEHHKKGRVFERLEMIDKE"
     gene            complement(2669..3175)
                     /locus_tag="VGB68_08865"
     CDS             complement(2669..3175)
                     /locus_tag="VGB68_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049381.1"
                     /translation="METGFTVKLPGRRNACNDEAFTGDLQSIVRKFYGSRAGEAPVDN
                     PATPDIDESLNKHSVSARGFDNLVTYFADLIALLQTQPAYNPNDAEMKIAALETKPAA
                     LQAKNNAAKAADIALGNALDARDEILYHPETGIVKLVKLIKTQLSRKPGKTSAAYQQI
                     NALEFRKY"
     gene            3483..4136
                     /locus_tag="VGB68_08870"
     CDS             3483..4136
                     /locus_tag="VGB68_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049382.1"
                     /translation="MNNMTFYLIIGACLIILLAGIMFLAFRSETKSSGLWGEKNSSPV
                     GGDGWSAEMGWRYVSYIEENTAIALQIEPMARGEDIVYVPDEANWLKSAPDWARQRRE
                     EILARLKSVMWNRRLVWQQGKGSFGASRFVPGSLESTWGGQRLEKRRMFHPGSKITHE
                     EAHELWHDAARLFAGQARGEVTIFKGAQGMPGTVFQEVELPALEINPNVTLVFKTPD"
     gene            complement(4262..5158)
                     /gene="ttcA"
                     /locus_tag="VGB68_08875"
     CDS             complement(4262..5158)
                     /gene="ttcA"
                     /locus_tag="VGB68_08875"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007972.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEY0049383.1"
                     /translation="MFVESKTKTKLLKKMGRALADFSMIEDGDKVMVCLSGGKDSYTM
                     LELLLDVQRRAPVKFEILAVNLDQKQPDFPQEVLPNYLESIDVNYRIVEEDTYSIVKA
                     HIPEGKTFCSLCSRLRRGILYSTAIEENCTKIALGHHADDIIATFLLNLFYVGQLKAM
                     PPILRSDAGTNTVIRPLAYCQEAEIAKFAAEKQFPIIPCNLCGSQPNLKRARVKRLID
                     ELEKEIPFIRSSIMTALSNVTGSHLLDTALYDFKNFEPIIKQIPRGHGSVEKELDEIF
                     DHSEANLAINKSSLPVIESLIG"
     gene            5484..6113
                     /locus_tag="VGB68_08880"
     CDS             5484..6113
                     /locus_tag="VGB68_08880"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY0049384.1"
                     /translation="MIRMGRSSKTEQSNETPQYQQQQQTTSGNVYGYQQETAPPPSSR
                     GAMTESESIARDIKEGRLSGFVGAGTVLTGETNFQAMLRIDGHLTGRVISDNGTLIIG
                     SGGQVDADILVSSATIGGTVNGDIVATEKVELGRTARVIGNIQTPRLMIEDGAIFEGN
                     CSMLKAKESAERRVEEARQAQYAVDEYQVETTAGYEEVTAEQSTEAAAS"
     gene            6312..6974
                     /locus_tag="VGB68_08885"
     CDS             6312..6974
                     /locus_tag="VGB68_08885"
                     /inference="COORDINATES: protein motif:HMM:NF016277.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049385.1"
                     /translation="MTERFSFINEEFFSLSAAPEQMDALWRQGWRHFGAHFFRYNLGF
                     YENEIRRVLPLRIRLADFKFSKSQRRVFNKNSDLNAVIRPVRIDAEKHSLFGRHKRRF
                     KSGVPDSIYDFLSEEPAVVPCEALEACVFERGNLLAASFFDIGKESVSSIYAMFEPSL
                     EKRSLGILTMLLEIRCAQECGKKFYYSGYAYEGNSYYDYKKRFSALESFDWNGNWEKF
                     AE"
     gene            7052..7612
                     /locus_tag="VGB68_08890"
     CDS             7052..7612
                     /locus_tag="VGB68_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049386.1"
                     /translation="MKKFTNPLDNIRVASPCPADWNEMLGDERKRFCSQCSLNVYNLS
                     GMTRDEAENLLLTSEGRVCVRFYRRADGTVLTKDCPVGWQAVKRRVSRTATAFISLCA
                     GIFTGIFSFNQPQTGTPNVAGATPVKRVAPPSFTEAIKSYEGDAFDSDYMGKPAITGD
                     LYAIPTAGMPSNSDEVRKDIKQKRKR"
BASE COUNT         1924 a         1689 c         1995 g         2072 t
ORIGIN      
        1 gcttgtgtgt attctgcggt cgtctctgtg taacgtgcgg tcgagagtgt aacttccgcg
       61 gtcgcttgtg tgtttgccgc ggtcaacagt gtaatctctg cggttcacag tgtctttgct
      121 gcggtcggga gtgtaattcg tgcggttgcc agtgtaaacg ccgcgcatct cagtgtattt
      181 cgcgcggtcg agagtgtaat ctttgcggtg acgagtgcaa atgtcgcgtt cgttagtgta
      241 atcatcacgg ttgcgagcgt gcagcgtgtt gattgtgata tatcggtagt ctgtcaaaag
      301 cgatatttat taataaaagg attgtatttc ccttatgaaa agtcattaac tgcctgtttc
      361 tttcgtcggc tcgcccgacc atttcagagg catccaataa accgagtctc cggtgtgaag
      421 cgtcgcgcct ctttccggct cataataagc cgccgcgctg ttggaataac tgccgcgttg
      481 ttttatcgct ctgaaagatt cgctgatgtc gcttaaatca aataatgaat gtgaagcgca
      541 aacggaaatc cgctttgatt ttttgttcac acgcataatt tgtcccggct gcggctccgg
      601 cgatttttcc gctttctcga caacatattc ctgatgcgct tcgagcttgc ggataacgcc
      661 gtcgccgaac ataaaagaaa cgctgatgga attgcagttc gttatgcgtc cgacagtttg
      721 ccagccgtcg cgcgaaaatc tgactctatc gccggtagct aaatatttgg ctttgatttt
      781 gtccataagg ttatgggctt tcggtatttg ccaagctcgg cgctgagtga ttaagcgacg
      841 gctccaagcc ggaaaagagc agggaaatca aacaacacaa ataaaaccaa gcctgtccgc
      901 agttttaggt tttcaatttc tttaatcgca tcttgtcttt ttacccgcga cggtttaagt
      961 tatctgtaat ttcatcaatg cccagagaaa ctaaaaacta aggcagatga aagccgtaat
     1021 aggaaaaaat ctaaaaatta cggctcaaca aaatagcaaa tacatactaa gactatttaa
     1081 tcttagcagc aagttaaaaa tagtgaagtt cgacatcgga ggtaaactct gatgggcaga
     1141 gcatcacttg aaaaacctga aagattagct gaaaaactat gtcagattcg taacgcttta
     1201 aatctttcac agaatgaatt aattcgttgg ttggacttaa ccgataaact aacccaagca
     1261 gaaatttcag catttgagcg cggagttaga attccgccgt tacttgtctt acttcgctat
     1321 gctcgaaaat tcaatataaa tgtggaaatt ctgattgatg atgaattaga tttgcctgaa
     1381 aagcttccga ataagctagt gaacaatcgt tgagaggttg gatgcctaaa tcatcgcaga
     1441 gccgaaaaaa gagcgaagca tcattaataa cggcgcgagt gtaaataaac agtgttcaaa
     1501 attaggtagt gtcctagttg tgtgttgcta agaaaattta tagccacgtc ctttagggcg
     1561 tggttagctt gaaggataaa acaaaagggc gtttcaactt ttgctccggc ttgagccata
     1621 agagtaagga cgttgaaacg cccttttatc tgatttatcg ttcgaccacg ccttaaagga
     1681 cgtggctatt tatcttagca gccccagatt aggacattat caaaaattat ctgttttgtg
     1741 agcaaataaa ttgataaatc tgttgaatca tttcttttgt agaatcaggc aattctaaat
     1801 attgaatttt atttaatgct ttttcgcaaa aatccacctt gtttttaaga gttcccgaat
     1861 cagtttgatt gcgctaattt taatcatatt tgatagattt ttgtgatttt tttatagcca
     1921 cgctgcttca gcgcgtggtc gggaatcgca agcattgata tagggcgttt taacgtcctt
     1981 ttattacaat actgatagcc aatccggtaa gcccgtttta acgggctgtg ttattaataa
     2041 tcttaaccac gcgctgaagc gcatggctat tgggaaataa tcaatagcgc tttgattatt
     2101 ctttatcaat catttccaat ctttcaaaaa ctcttccttt tttgtgatgc tctttttgat
     2161 tcagaacata attcacgacc cattttaaat ctttcgtgcc gaaacttacg atgccgtagc
     2221 cgtgctgcca ttccagcaat ttacggttgg ctagttggtt gatgtaatgc gagcttgagc
     2281 cttttaattg accaatccat tcggcgggct ggatggtcgg cggaacgcta acggcaatgt
     2341 gaatatgatt ttcgatgcct ccggcggcgt gaaaaatcgt ttcgggcgtt tcaagaattc
     2401 tgtctttcag gaattggtag agtttcggtt cgagttcggg tttaatcgac ggcagattcg
     2461 attttgtatg ccatgtgata tggaaattga tttccgaata tgctctgcgc gacatagttt
     2521 ttggaaggac gttaaaacgc cctttatggt gtttttgaac gtgaccacgc gctaaagcgg
     2581 cgtggctaat taatttgtca aaaaatgaag gatgaatcaa gccaaaaagc tatttccggt
     2641 ttattggtca tccttcatta atttccaatc aatatttcct aaactcaagc gcgtttatct
     2701 gttgataggc ggcgctggtt ttgccgggtt tgcgggagag ttgggttttg attagtttga
     2761 cgagtttgac gatgcccgtt tcggggtggt agagaatctc gtcgcgggcg tcgagggcgt
     2821 tgccgagggc gatgtcggcg gctttggcgg cgttgttttt ggcttggagc gccgccggtt
     2881 ttgtttcgag ggcggcgatt ttcatttccg cgtcgttcgg gttgtaggcg ggctgcgttt
     2941 gcagcagggc gatgaggtcg gcgaaatagg tgacgaggtt gtcgaagccg cgcgccgaga
     3001 cggaatgctt gttgagtgat tcgtcgatgt cgggcgtggc gggattatcg acgggcgctt
     3061 cgccggcgcg cgagccgtaa aatttgcgga cgattgattg caaatcgccg gtaaaggctt
     3121 cgtcgttgca ggcattgcgc cgcccgggca attttacggt aaatccggtt tccaaaaaac
     3181 acaggtctgt tgcgatgcgg gaaatataaa ttaaaagcgc gcgaagtcaa tatttttatt
     3241 tcacccgcac ggcacggcgc gcctttcggc gcggaaaaat tattggtaaa aaaagtctgt
     3301 tggcatataa taactgcgcc cgtcaccaca ggtgaatatg aaaacgtcac gggcgagcaa
     3361 tttcccgctt ccttcggcat tcgtgcctta aaaaataatt cgccgccggg atgatacggg
     3421 attagttttc cttaattcag agtttgatta tttcagctat cctgaacagg aaagatttga
     3481 atatgaacaa tatgacattc tacctcatta tcggcgcgtg cctaattatt ttgctggcag
     3541 gcattatgtt tttagccttc aggagtgaaa cgaaaagttc gggcttatgg ggcgagaaaa
     3601 actcaagccc ggtcggcggc gacggctggt cggctgagat gggctggcga tacgtcagct
     3661 atattgagga aaatactgcg atagctttac aaatcgagcc gatggcgaga ggtgaagata
     3721 tcgtgtatgt tccggacgag gcgaactggc tgaaatccgc gccggattgg gcgcggcaga
     3781 gacgagaaga aattctcgcc cggctcaaat ccgttatgtg gaatcgcagg ctggtctggc
     3841 agcaagggaa agggtctttc ggcgcgagcc gtttcgtgcc cggttcgctg gaatcgacct
     3901 ggggcgggca gagactggaa aagcggcgta tgtttcatcc gggcagcaaa attactcacg
     3961 aggaagcgca cgaattatgg cacgacgcgg cgcgtttatt tgccggtcag gcgcgaggcg
     4021 aagttacaat ttttaaagga gctcaaggca tgcccggcac tgtttttcag gaagtagagc
     4081 tgcccgcgct ggaaattaat ccgaatgtga ctctggtttt caaaacaccg gattaaaaga
     4141 ttgctcccgg cttttctcga attctcgtaa ttaaacactt ttgcccgaca tatataaaaa
     4201 aaggattgaa gcagccagct tcaatccttt ttttaataag atttttcacg cggaaaaggc
     4261 tttacccgat aagcgattcg ataaccggca ggctgctttt gtttatcgcc agatttgctt
     4321 ccgaatggtc gaaaatttcg tcgagttcct tttcgaccga gccgtgaccg cgcgggattt
     4381 gtttgataat cggctcgaag tttttgaaat cgtaaagcgc ggtgtcgagc aggtgcgagc
     4441 cggtgacgtt ggaaagcgcc gtcataatcg acgagcggat aaagggaatt tccttttcca
     4501 gctcgtcgat taatcttttg acgcgggcgc gtttcaggtt cggctgcgag ccgcacaggt
     4561 tgcagggaat aatcggaaat tgtttttcgg cggcgaattt ggcgatttcc gcttcctgac
     4621 aataggcgag cgggcgaatg acggtgtttg ttccggcgtc ggaacgcaaa atcggcggca
     4681 tcgcttttaa ctgcccgacg tagaataaat tcagcagaaa cgtggcgatg atgtcgtcgg
     4741 cgtgatgccc gagcgcgatt ttcgtacaat tttcttcaat cgccgtcgaa tacaaaatgc
     4801 cgcgtcgaag gcgcgaacaa agcgagcaga aagttttgcc ttcgggaatg tgcgctttga
     4861 cgatcgaata cgtgtcttcc tcgacgatgc ggtaattgac atcgatgctt tccagatagt
     4921 tgggcaaaac ttcttgcggg aaatccggct gtttctggtc gagattgacg gcgagaattt
     4981 cgaatttaac cggcgcgcgc cgctgcacgt cgagcagtaa ttcgagcatc gtgtagctat
     5041 ccttgccgcc cgacaggcac accatcacct tgtcgccgtc ttcaatcatc gaaaaatcgg
     5101 cgagcgctct gcccattttt ttgagtaatt tcgttttcgt cttgctttct acaaacaact
     5161 tttataaagc tccttattcg ttggttagtt gtcggttacg ggcagccggt tgccggttga
     5221 atgaaaacgg cgttcccgaa tttatttcga ctgataaaca ggcttcgcgg ctttcgtcga
     5281 cgacggacaa cccgaaattt gccactttac tacaaattcc gccaaaaacc tagacacgcc
     5341 gccgcaaagc atctattata aaacaaccga ctggaagtct tgatttcctt aaaaattaaa
     5401 atcaacaatt aaaaagactc gcgtttactt tggagcaggc gatttccaat tatcattcgc
     5461 cgggtcacaa gaggttttta tatatgatta gaatggggcg aagttccaaa acggaacaat
     5521 cgaatgaaac accgcaatac cagcagcagc agcaaacaac ttcggggaac gtttacggct
     5581 atcaacagga aaccgctccg ccgccgtcat cgcgcggcgc gatgaccgaa agcgaatcga
     5641 tcgcgcgcga tatcaaggaa ggacgcctga gcggctttgt cggcgcgggc acggttttga
     5701 cgggcgaaac gaattttcag gcgatgctgc gtattgacgg gcacctgacg gggcgcgtca
     5761 tttccgataa cggcacgctg ataatcggtt cgggcgggca ggtcgatgcc gatattctcg
     5821 tttcgtcggc gacgatcggc ggcacggtca acggcgatat cgtggcgacg gaaaaggtcg
     5881 agctcggacg cacggcgcgc gttatcggta atattcaaac gccgcgcctg atgattgaag
     5941 acggcgcgat ttttgaaggc aactgctcga tgctcaaagc gaaggaatcc gccgagagac
     6001 gcgtcgagga agcccgccag gcgcagtatg ccgtcgatga atatcaggtg gaaacgaccg
     6061 ccggctacga agaagtgacc gccgaacagt ctacggaagc ggcggcgagc tagccggtaa
     6121 acaacgacga atagtgaata ggcgacggat atttagatcg gctattcgct atttttgttt
     6181 tcacgcggaa aagcgaaaac gattcagagg cggtagacga taagcgccgg gcgaaagaaa
     6241 cgaatttgcc gtttaccgcc aaatcgccgc cggcatttcg gttcgcgtga tttaaaatat
     6301 tttcattcgc gatgaccgaa cgattttctt tcattaacga agaatttttt tccctgagcg
     6361 ccgcgcccga acagatggac gcgctctggc ggcaaggctg gcggcatttc ggagcgcatt
     6421 tttttcgcta caatctgggc ttttacgaaa atgagattcg ccgcgttctg cctctcagga
     6481 ttcggctcgc cgattttaaa ttttccaaaa gccagcgccg cgtttttaat aaaaacagcg
     6541 atttgaacgc ggtcattcgt cccgtccgca tcgacgcgga aaagcacagc ctgttcgggc
     6601 gtcacaagcg gcgtttcaag tccggcgtgc ccgattcgat ttacgatttt ctttccgaag
     6661 agccagccgt cgttccgtgc gaagcgctcg aagcttgcgt tttcgagcgg ggaaatctgc
     6721 tcgcggcgag ctttttcgac atcgggaaag aatctgtttc gagcatttac gcgatgttcg
     6781 agccgtcgct ggaaaagcgc agtctcggca ttttgacgat gcttctggaa attcgctgcg
     6841 cgcaggaatg cgggaaaaag ttttattatt ccggctacgc ttacgaggga aattcttatt
     6901 acgattataa aaaacgattt tccgcgctgg aaagcttcga ttggaacggc aattgggaaa
     6961 agtttgccga ataattttgc aagcggtaaa tttttctttc gttcttcggg aacaaaagac
     7021 gttttcgggc gtctaaagaa tcagaagctt tatgaaaaaa tttaccaatc cgcttgataa
     7081 tatccgcgtt gccagcccgt gcccggcaga ttggaacgaa atgctcggcg acgagcgaaa
     7141 gcgtttttgc tcgcagtgca gtttaaacgt ctataatctt tcgggaatga cgcgcgacga
     7201 ggctgaaaat cttttgctga cttcggaagg tcgggtctgc gtcagatttt acaggcgcgc
     7261 ggacggcacg gttttaacga aagattgccc ggtcggctgg caggctgtca agcgccgcgt
     7321 gtcgcggacg gcgaccgcct ttatttcgct gtgcgccgga atctttaccg gaattttctc
     7381 ctttaatcag ccgcagacgg gaacgccgaa cgtcgccggc gcgactccgg ttaaacgtgt
     7441 cgcgccgccg tcatttaccg aggcgattaa aagctacgag ggcgatgctt tcgactccga
     7501 ttatatggga aaaccggcga ttacgggaga tttatatgca atcccgacag ccggaatgcc
     7561 ttcgaattca gacgaggtca gaaaagatat caagcaaaag cggaaacgtt aaaaaataaa
     7621 aaaaatgccg ggtgttttta tttaatgaaa catccggcat tttttattca tttttatttt
//
LOCUS       DASWNE010000443         4790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_84330, whole genome shotgun sequence.
ACCESSION   DASWNE010000443 DASWNE010000000
VERSION     DASWNE010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84330"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VGB68_08895"
     CDS             complement(<1..189)
                     /locus_tag="VGB68_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049387.1"
                     /translation="MKPELIENKEIYQGKIFDVSLATVREENITYDREIVSHPGSAVI
                     VPVFADKTVALVKQYRHAA"
     gene            275..784
                     /locus_tag="VGB68_08900"
     CDS             275..784
                     /locus_tag="VGB68_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049388.1"
                     /translation="MDSYLEKPHARTGNRKNFIGTVIVALFLVIATSGFYSTCTSRQA
                     LQQQQLEGAVREGTPEFEALTKKIVIQTDDNNTMQSPTGMGTIVMMIAGKIRNFSGKT
                     LTGLEIKVTVVDQLEKPVKDKTLVVVPNQQPTLETNQTMPVRIMIEGFSKDDDRAMIR
                     WKVTAIKTE"
     gene            complement(909..1844)
                     /locus_tag="VGB68_08905"
     CDS             complement(909..1844)
                     /locus_tag="VGB68_08905"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0049389.1"
                     /translation="MNYIVEIENLTKDYETGFWKKKKTRALDGLSLQVEGGQIFGFLG
                     GNGAGKTTTIKLLMRLLFPTSGTARILGRDIADVKMHRAIGYCPENPYFYDYLTAREL
                     MMYFGELFGYDSQTRRRKTEELLTRVGLDEKEWKKQLRKFSKGMLQRVGLAQALINEP
                     EIVFLDEPMSGLDPIGRREIRELITDLRAAGTTVFMSTHILSDIEALCDRVAILRNGK
                     LAATGKLDELLTQSGETQGFEVNLKGVSAEMINEKLDAIAAGVSAKPNGISIQVLSEA
                     DVEKVLAISKAAGGRLISVQPVKQSLEELFVKETS"
     gene            complement(2004..2933)
                     /locus_tag="VGB68_08910"
     CDS             complement(2004..2933)
                     /locus_tag="VGB68_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049390.1"
                     /translation="MKSNPAKIISATAIIVLGFAAIFALSRFIETNRPALPGGFEDSD
                     LAYQGAKLKNYSLGFNGLLADFYWMQALQYIGNKTLNSKEKVDMENLRPLNPRLLYPL
                     LDNATDLDPQFTTVYSYGAVVLPAIDPQQAIKFTEKGIRDNPGEWRLYQHLGFIYWKT
                     GDYEKASEVYGDGAKLRGAPAWMQIMSARLKAEGGSRATSREIYRQMLAETENAEVKE
                     NAAARLLQLDSMDEREAIRSALENFKSKNNRCANAWRELLPLLQTVKLPDGKSFRVDN
                     ASNPADPTGAPYILDKENCDVKLDAEKTRLPLQ"
     gene            complement(3092..3940)
                     /locus_tag="VGB68_08915"
     CDS             complement(3092..3940)
                     /locus_tag="VGB68_08915"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0049391.1"
                     /translation="MNAVIEDVFSKSNLQTMPIPENQNSVFRRIFSIARNTFREAVRD
                     RVLYNLVVFVLLITASAIFLGELTAGHEARTIVNLGLSAMLLFGAFIAIFVGVSLVSK
                     EIEKRTVFAIFAKPIGRGEFIVGKYLGLCLTLLVNVLVMGVGVSLAIFFVGGGHLALS
                     VWGAVFLIFLELAILTAVAILFSSFSSPALSALLAFFVFGIGHFSASLRDLAEYLGSG
                     LARVFFGFFYIILPNLSHFSFITNAAHGDVPNGAMLGGAFLYAIVYVVILLTITTLVF
                     SRRNFK"
     gene            complement(4022..>4790)
                     /locus_tag="VGB68_08920"
     CDS             complement(4022..>4790)
                     /locus_tag="VGB68_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0049392.1"
                     /translation="FFFVLALATYEMQKIFRKKKKPLPTFSFQPKPVLAFSFSLAALF
                     FVISLSANLSNQYVLSAENAEDFAVGEDFYRRAIRLNPRNASAYFSYGQQLYFKKQPE
                     KAVPMFEKAIENGLDASIAYSYLASAQAAAQNPLAAEETIAQALRIYPRSTFLRVRYA
                     TLLEKKGEMKKSDEQMEIARRINDKQANGWQELLTTGAAATALKARSDEKIIAPHELL
                     PTACLYAVLDEQAVENRFPRNGLLVTNKENFAAGNLK"
BASE COUNT         1212 a         1200 c         1191 g         1187 t
ORIGIN      
        1 ggcggcgtgg cggtattgtt tgaccagcgc gacggtttta tccgcaaaaa ccggcacgat
       61 gaccgcgctt ccgggatgcg aaacgatttc gcggtcgtat gtaatgtttt cctcgcgcac
      121 ggtggcgagc gaaacgtcaa aaattttgcc ttggtaaatt tccttgtttt cgataagttc
      181 gggtttcata agtttgataa ttttcttaaa atgctaactt acagggaact tttttccaaa
      241 accgccatct aatcaaccag taaaggaaac agctatggat tcatatttag aaaagccgca
      301 cgcccgaacc ggaaaccgca aaaactttat cggaacggtg atagtcgcgt tatttctcgt
      361 catcgccacc agcggatttt attcgacctg cacgtcgagg caggctttgc agcagcagca
      421 attggaaggc gcggtgcgcg aaggcacgcc ggaatttgaa gcgctgacga aaaaaatcgt
      481 cattcaaacc gatgacaaca acacgatgca gtcgccgacc ggaatgggca cgattgtgat
      541 gatgattgcc ggaaaaatcc gcaatttttc gggcaaaact ttaaccggct tggagattaa
      601 agtgacggtc gtcgaccagc tcgaaaaacc cgttaaagac aaaactctgg tggtcgttcc
      661 gaaccagcag ccgacgctcg agacgaatca gacgatgcct gtcaggatta tgattgaagg
      721 ttttagcaaa gacgacgacc gcgcgatgat tcgctggaaa gtgacggcga taaagactga
      781 ataacggata acggataact gataactgaa agaagaattt ttatttttag ttgtcagctt
      841 ttaattatca gttatcagtt atcagttatc aactatcaac tgtcaactat cagttatccg
      901 ttatcagttt atgaagtttc ttttacaaat aattcttcca gcgattgttt aaccggctgt
      961 accgaaatca gcctgccgcc cgcggctttg gagatagcca gaactttttc gacgtccgct
     1021 tcggataaaa cctgaatgct gatgccgttc ggtttcgcgg aaacgccggc ggcgattgcg
     1081 tccaatttct cgtttatcat ctccgccgaa actcctttca gattgacttc aaagccttga
     1141 gtctcgccgc tctgcgtcag taattcgtcg agctttccgg tcgccgccag ttttccgttt
     1201 cgcaaaatcg ccacgcggtc gcagagcgct tcgatgtccg acaaaatatg cgtggacata
     1261 aaaacggtcg tgcccgccgc gcgcaaatcc gttatcaatt cgcgaatctc gcgcctgccg
     1321 atcgggtcga gaccgctcat cggctcgtcg agaaaaacga tttccggctc gttgattaaa
     1381 gcctgcgcca agccgacccg ctggagcatt cccttggaga atttgcgaag ctgttttttc
     1441 cactcttttt cgtccagccc gacgcgcgtt aaaagctctt cggtttttcg cctgcgcgtt
     1501 tgcgagtcgt agccgaaaag ctcgccgaaa tacatcatca attcgcgcgc ggtcagataa
     1561 tcgtaaaaat atggattttc ggggcaatag cctatcgcgc ggtgcatttt cacgtcggca
     1621 atatcacgac ctaaaattcg cgccgtgccg gaagtcggaa acaaaagccg catcagaagc
     1681 tttatcgtcg tggttttgcc cgcgccgttg ccgccgagaa agccgaaaat ctgcccgcct
     1741 tcgacctgca ggctcaaccc gtcgagcgcg cgcgtctttt tctttttcca gaagccggtt
     1801 tcgtaatctt tggttaaatt ttcgatttcg acaatgtaat tcataataaa atcagaaatt
     1861 aatcacagag aaaatccaaa caggacgcgc aggatttacg ggcgaaaaga attttctctc
     1921 cgctcgcttt cgtgtttgtt cgtggctaaa tcgcttttca taatctcggt agtaaaaatt
     1981 ttattatctc ggtagtaaaa attttattgc aaaggcagcc gggttttttc cgcgtccagt
     2041 tttacgtcgc agttttcctt gtccagaatg tagggcgcgc ccgtagggtc agccggattc
     2101 gatgcgttat cgacgcgaaa gcttttgccg tccggcagct ttaccgtttg cagaagcggc
     2161 aaaagctcgc gccaggcgtt agcgcagcga ttgttctttg atttgaagtt ttccagagcg
     2221 ctgcggattg cctctcgttc gtccatcgaa tcgagctgca aaagccgcgc cgccgcattt
     2281 tctttgactt ccgcattttc ggtctcggcg agcatctgcc ggtagatttc gcgcgaagtg
     2341 gcgcggcttc cgccttcggc tttcaatctg gcgctcatta tctgcatcca ggcgggcgcg
     2401 ccgcgcagtt tagcgccgtc gccgtaaact tcggaagctt tttcgtaatc gcccgttttc
     2461 cagtagataa aaccgagatg ctggtaaagc cgccattcgc cgggattgtc gcgaatgcct
     2521 ttttccgtaa attttatcgc ctgctgcggg tcaatcgccg gcagcacgac cgcgccgtag
     2581 gaataaacgg tcgtaaactg cgggtcgagg tcggtcgcgt tgtcgagcag cggataaagc
     2641 aggcgcggat taagcgggcg caggttttcc atatcgacct tttccttgct gttgagcgtt
     2701 ttgttgccga tatactgcaa agcctgcatc caatagaaat ccgccagcaa accgttaaag
     2761 ccgagactgt aatttttcag cttcgcgccc tgataagcca aatccgaatc ttcaaatccg
     2821 ccgggcaaag ccggacgatt cgtttcgata aaccggctca acgcaaaaat tgccgcgaaa
     2881 ccgagaacga ttatcgcggt cgccgagatg atttttgccg ggttcgattt cataaaatca
     2941 atttaatcca aaagggaatt caaacaggat ggacaggatg aacaggataa tttaaaacta
     3001 tgaacgttga attttatttt ttatcctgcc tatcctgtcc atcctgttaa aatccgcgtt
     3061 tacctgcgaa aatctgtggt ttcattttct cttatttaaa gtttcgtcgg ctgaaaacca
     3121 gtgtcgtgat ggtcagcaaa attacgacgt agacgattgc ataaagaaat gcgccgccga
     3181 gcatcgcgcc gttcggcacg tcgccgtgcg cggcatttgt aataaagctg aaatgcgaaa
     3241 gattcggcag tataatataa aaaaaaccga aaaagacgcg cgccaagccc gagcccagat
     3301 actcggctaa atcgcgcagc gaggcgctga aatgcccgat accgaaaaca aaaaacgcca
     3361 gcagcgcgct caatgccggc gaggaaaacg aggaaaacaa aatagcgacg gcggttaaaa
     3421 tcgccagttc gagaaaaatc agaaagaccg cgccccagac cgaaagcgcc agatgtccgc
     3481 cgccgacgaa aaaaatcgcc aggctgacgc cgacgcccat caccagaacg ttgaccaaaa
     3541 gcgtcaggca caagcccaga tattttccga cgataaactc gccgcgtccg atcggcttgg
     3601 cgaaaatcgc gaaaacggtg cgcttttcga tttctttcga aaccaggctg acgccgacga
     3661 aaatcgctat aaaagcgccg aaaagaagca tcgcgctcaa gccgagattg acgatggtgc
     3721 gcgcctcgtg tccggcggtc agctcgccga gaaaaatcgc gctcgccgtg ataagcagca
     3781 cgaaaacgac cagattatat aaaacgcggt cgcgcacggc ttcgcggaaa gtgtttcggg
     3841 cgatggaaaa aatccttcgg aaaacactgt tttgattttc gggaatcggc atcgtttgca
     3901 gatttgactt tgagaaaacg tcctcgatta cagcgttcat atcttaacac agaaaaaggg
     3961 aattttttga aggcgaaagg caaaagtaaa aaggcgaaaa gcatttttta cctgtgtctt
     4021 tttacttcag attcccggcg gcaaaatttt ctttgtttgt taccagaagt ccgtttcggg
     4081 gaaagcgatt ttcgactgcc tgctcgtcga ggacggcata caggcaggcg gtcggcagca
     4141 gttcgtgcgg cgcgatgatt ttttcgtcgc ttctggcttt taacgctgtc gcggcggcgc
     4201 ctgtcgttag taattcctgc cagccgtttg cctgtttatc gttaatccgg cgggcgattt
     4261 ccatctgctc gtccgatttt ttcatctcgc cttttttctc taaaagagtg gcgtagcgaa
     4321 cgcgcagaaa tgtcgagcga ggataaattc tgagagcctg agcgatggtc tcttcagccg
     4381 ccagcgggtt ttgcgccgcc gcctgcgccg aagccagata agaataggcg atgctcgcgt
     4441 ccaagccgtt ttcgatggct ttttcaaaca tcggcacggc tttttccggc tgctttttaa
     4501 aataaagctg ctgcccgtaa gagaagtaag ccgaagcgtt tcgcggatta agcctgatag
     4561 cccgccggta aaaatcttcg ccgacggcaa aatcttccgc gttttccgcg cttagaacgt
     4621 attggttgct cagattggcg gaaagggaaa tgacgaaaaa taacgccgcg aggctgaagg
     4681 aaaaagccag aaccggtttc ggctgaaacg aaaaggtcgg cagcggcttt ttcttttttc
     4741 ggaaaatctt ctgcatttca tacgtcgcca gcgccagcac gaaaaagaaa
//
LOCUS       DASWNE010000444         5548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_15267, whole genome shotgun sequence.
ACCESSION   DASWNE010000444 DASWNE010000000
VERSION     DASWNE010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5548
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15267"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3537)
                     /locus_tag="VGB68_08925"
     CDS             complement(<1..3537)
                     /locus_tag="VGB68_08925"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY0049393.1"
                     /translation="MRKTVSFLLIFAIYLGMPAPLVLQVNAQKTSGGKTIQTYMSELP
                     EGLKFRLSEGVEGAESREKQAPAKGDALSEGETSSLLKRIPEIKTDAEDRKAFNKRAG
                     SLTPPKTGKMVPVKFPAPDERDAPNVEASKQALQVLRFSPEGEISLAPDLSVTFSQPM
                     VAVTSQEQAAQVVPVQLTPNAEGNWRWLGTKTLMFDTTKRFPMATRFTARVPAGTKSA
                     TGQILQKDVTWTFTTPPPKVEQMIPANQTTRRDALMFISFDQEISPEAVLRTITVAGG
                     GKKLPVRLATQQEIDADGSLAYYAKQAQPKRWLAFRAVNSDGLTENALPADAAITVTV
                     NKGTPSAEGPLTTTKDQSYSFRTFGAMKFAGGYCGWRGNKNCSPFETWYLEFTNEINA
                     SNFTKEMVKVEPAVEGLKIYPSGNYIFFEGYKKGKTTYKISVDNRLKDVYGQNLVAPA
                     AATIAVGSAQQNLYTQGGNFVVLDPTAKPAYSIYSTNHRSVKVKIYAVQPTDWYQFQK
                     YVRYINYDDGTKKPPIPGRLIVDKVVTIENKPDEMVETRIDLTEALNDGFGHAIVDVE
                     PTVRRDKYDKTRVLAWAEATQIGLDAFVDNQELIGFATDLKTGKPLSGVELAIYPNGK
                     AISGQRSAVSEEENAGKSWWEWLTTWGASEESINKETEAVEENGEPTKTEIVNEAQTN
                     QTTANGILRLALPEEAAKQPNILIAKKGKDVAFMPEQSDYYWQDYGNWHKKGVADSLR
                     WFVFDDRKMYRPKEEVSVKGYIRVYEGGKFGDIQPLGNRASTVFYAVRDAQNNEVAKG
                     QANLNSFGAFDFKFKLPDNMNLGNASISLGANGDAYSQTHIFQVQEFRRPEFEVTTKV
                     ETEAPHFVGASANVAVEAKYYAGGGLANADANWSVTATPTSYTPPNRGDFTFGTWIPW
                     WRMSNYDYDGESSMRGGYNPRGGSTSQAFKGVTDAGGRHLLKIDFESVNPPRPYSIRA
                     AASVQDVNRQTWSSTTSLLVHPSSLYAGLKISRYFVQKGDPIEVESIVSDIDGKLIAG
                     RDVEIKAVLKDWAFDKGTWQEQIVDEQSCAVKSTEKAEKCKFVAKQGGRYTITATVMD
                     DRERFNQTEITIWVAGGKTPPKRNVEQEEAQIIPNKKDYAPGDVAEILVMSPFPQAEG
                     VLTLRRDGLVKTERFTMKESSITLKIPIEEKYL"
     gene            complement(3716..5068)
                     /locus_tag="VGB68_08930"
     CDS             complement(3716..5068)
                     /locus_tag="VGB68_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049394.1"
                     /translation="MNDKLAEAVRTLDERILTNHRFDKKLFAELDEAQRELGILSGER
                     PFCPFLRPAFFSRKIYEKITSAAEVLGGALEKMTLGALENEEIMRELDLTEREERLAR
                     IDPRYKTVCNSSRFDAFAHGEDFKFLEYNGETPAGIIDQMQIEKVMELIPEVREFLSE
                     HKHRRPKPHVKLLAALVSDYRDFGGKKEKPNIAIVDWNGVSTYSEFVVLREFFQSEGH
                     ESIIADPHELEFDGRVLRVGAFEIDIFYKRVVIQEFLEKFPEDNPLSNAYRAGKVCML
                     NSFRTKIPNKKASFAILSDKKYAYLFTAQELEMIEKHIPWTRRVRDAKTDFGGKEVDL
                     LEKLRRERERFLLKPNDDYGGNGIVLGWERSAGEWETALQNALAESFVAQERVAVEKV
                     MFPTYSDEAETKELLIDFDPFLFGGKAEGGLVRLSSSSLVNVAQGGGETALIVLENAF
                     "
     gene            5276..>5548
                     /locus_tag="VGB68_08935"
     CDS             5276..>5548
                     /locus_tag="VGB68_08935"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEY0049395.1"
                     /translation="MGLWARITRIFRASTGAALDKMENPELVLQQTIRDMRDRVPELN
                     NSVAQVMATERLLAKNKENLANQVVDLDSKIKASVKMGRDDIATAYI"
BASE COUNT         1166 a         1494 c         1395 g         1493 t
ORIGIN      
        1 cagatatttt tcttcaatcg gaatctttaa agtgattgat gattccttca tcgtgaaacg
       61 ctcggttttc accagtccgt cgcgccgcag ggtcaaaacg ccttcggctt gcggaaacgg
      121 cgacatcacg agaatttccg ccacgtcgcc cggcgcgtaa tcttttttgt tcgggatgat
      181 ttgcgcttct tcctgttcga cgttgcgttt gggcggcgtt tttccgcctg cgacccaaat
      241 cgtgatttcg gtttgattaa aacgctcgcg gtcgtccatc accgtcgcgg ttatcgtgta
      301 gcgcccgccc tgtttggcga cgaatttaca tttttccgct ttttcggtcg attttacagc
      361 gcagctttgc tcgtcgacaa tctgttcctg ccacgtgcct ttatcaaacg cccaatcttt
      421 taagacggct ttaatttcca cgtcgcgacc ggcaatcagt tttccgtcga tgtcggaaac
      481 gattgattcg acctctatcg ggtcgccttt ctgaacgaaa taacgcgaaa tttttaaacc
      541 ggcgtagagc gacgacgggt gaacgagcag gctggtcgtg cttgaccacg tttggcggtt
      601 cacgtcctgc acggaagccg cagccctgat agaataagga cgcggcggat tgaccgattc
      661 aaaatctatt ttcagaagat gcctgccgcc ggcgtcggtc acgcctttaa aagcctgcga
      721 agtgctgccg ccgcgcggat tgtagccgcc gcgcatactg ctctcgccgt cgtaatcgta
      781 attgctcatt cgccaccacg gaatccacgt tccgaacgta aaatctccgc gattcggcgg
      841 cgtatagctg gtcggcgttg cggttacgct ccagttcgcg tcggcgtttg ccagaccgcc
      901 gcccgcatag tatttcgctt cgacggcgac attggcgctc gcgccgacaa aatgcggcgc
      961 ttcggtttcg actttcgtcg tcacttcaaa ttccggtcgg cgaaattcct gcacctggaa
     1021 tatgtgcgtc tgggaatatg catcgccgtt cgcgcccaga ctaatcgagg cgttgccgag
     1081 attcatattg tccggcagct tgaatttgaa atcgaacgcg ccgaaggaat ttaaattcgc
     1141 ctgacccttg gcgacttcgt tgttttgcgc gtccctgacc gcgtaaaaaa ccgtgctcgc
     1201 gcgattgccg agcggctgaa tatcgccgaa ttttccgcct tcgtagacgc gtatgtagcc
     1261 tttaacggaa acttcctctt tcgggcgata cattttccgg tcgtcaaaga cgaaccagcg
     1321 caagctgtcg gcaacgcctt ttttatgcca gttgccgtaa tcctgccagt aataatcgga
     1381 ttgttcgggc ataaacgcga cgtctttgcc ttttttcgca atcaatatat tcggctgttt
     1441 tgccgcttcc tcgggcagag caagccgcaa aattccgttg gcggtcgttt gattcgtctg
     1501 cgcttcattg acgatttcgg ttttcgtcgg ctcgccgttt tcctcgacgg cttcggtctc
     1561 tttatttatg gattcttcag acgcgcccca ggtggtgagc cattcccacc agcttttacc
     1621 ggcgttttct tcttcgctga ccgccgaacg ctgcccgctg attgcttttc cgttcggata
     1681 aatcgccagc tcgacgcccg aaagcggttt tccggttttc aaatccgtcg caaagccgat
     1741 taattcctga ttatcgacaa aagcgtcgag cccgatttgc gtcgcttccg cccaagccag
     1801 aacgcgcgtt ttatcgtatt tatctcgccg gacggtcggc tcgacgtcga caatcgcgtg
     1861 accgaaaccg tcgtttaggg cttccgtcaa atcgatgcgc gtttcgacca tttcgtccgg
     1921 cttgttttcg atggtcacga ctttatcgac gattaatctg cccggaatcg gcggtttctt
     1981 cgtgccgtcg tcgtagttta tataacggac gtatttttga aattgatacc aatccgtcgg
     2041 ctggacggcg tagattttca ctttgaccga gcgatgattg gtcgaataaa tcgagtaagc
     2101 cggctttgcc gtcgggtcga gcacgacgaa atttccgccc tgcgtgtaaa gattctgctg
     2161 ggcggaaccg accgcgatgg tcgccgcggc gggcgcgacc agattttgcc cgtaaacgtc
     2221 ttttaagcgg ttatcgaccg agattttata agtcgttttg ccttttttgt aaccttcaaa
     2281 gaagatgtaa tttccgctcg gataaatttt caagccttcg acggcgggct cgaccttgac
     2341 catttctttt gtgaaattcg acgcgtttat ctcgtttgta aattccagat accacgtctc
     2401 gaaaggcgag cagtttttat tgccccgcca gccgcagtag ccgccggcga acttcatcgc
     2461 gccgaacgtg cggaagctgt aactctggtc tttggtcgtc gttaaaggac cttccgcgga
     2521 aggcgttcct ttgttgaccg taacggtgat tgccgcgtcg gcgggcagag cgttttccgt
     2581 caaaccgtcg gagttaacgg cgcgaaaagc gagccagcgt ttcggctgcg cttgttttgc
     2641 gtaataagca aggcttccgt ccgcatcgat ttcctgctgc gtcgccagac ggacgggcaa
     2701 tttttttccg ccgccggcga cggtgatcgt ccgcaaaacg gcttccgggc tgatttcctg
     2761 gtcaaacgag atgaacatca gcgcgtcgcg ccgcgtcgtc tgatttgccg gaatcatctg
     2821 ctcgactttc ggcggcggcg tggtgaaagt ccacgtcacg tctttttgca aaatttgtcc
     2881 ggtcgccgac tttgttccgg cgggcacgcg cgcggtgaat ctggtcgcca tcggaaagcg
     2941 cttcgtcgta tcgaacatca gggtttttgt gccgagccag cgccagttgc cctccgcgtt
     3001 cggcgtcagc tggacgggaa cgacttgcgc ggcttgttcc tgcgaagtga cggcgaccat
     3061 cggctgcgag aaagtcacgc tcaaatccgg cgcgagcgaa atttcgcctt cgggcgaaaa
     3121 gcgcaaaacc tgcaaagcct gtttgctcgc ctcgacgttc ggcgcgtcgc gctcgtcggg
     3181 cgcgggaaat ttaacgggaa ccattttgcc ggttttcggc ggcgttaagc tgccggcgcg
     3241 cttgttgaaa gccttccggt cttccgcgtc cgttttaatt tcgggaatgc gctttaaaag
     3301 gctcgacgtc tcgccttcgg acaaagcatc gccttttgcg ggcgcttgtt tctcgcggct
     3361 ttccgcgcct tcgacgcctt cgctcaggcg aaattttaat ccttccggca gctcactcat
     3421 atatgtttgg atggtttttc cgccgctcgt tttctgcgcg ttgacttgca gaacgagcgg
     3481 cgcaggcatt cccagataaa tagcaaaaat caataaaaaa gaaaccgttt ttctcataat
     3541 tatccttcga taaaacttta gagagcgcaa attccgagga gagcctttcg cgcctcatat
     3601 taccttaaat tctgttttca aaccagcttt gcaaaactca gccgtcgata gcagaacgcc
     3661 tttcgaattt aaaacttgca ggattcattt tcgagaaaat tcggcgaatt ttatactaaa
     3721 aagcattttc caaaacgatt aaagccgttt cgccgccgcc ttgcgcgacg ttgacgagcg
     3781 aagacgaaga aagacgcacc agtccgcctt ccgccttgcc gccgaacaaa aacgggtcga
     3841 aatcaatcaa taattctttt gtttcggctt cgtccgaata cgtcggaaac ataactttct
     3901 cgaccgccac tctttcctgc gcgacgaacg attcagcgag cgcgttttgc aaagccgtct
     3961 cccattcgcc cgccgacctc tcccagccta aaacgattcc gttgccgccg taatcgtcgt
     4021 tcggttttaa gagaaatctt tcgcgctcgc ggcgcagttt ttcgagcaaa tcaacttctt
     4081 tgccgccgaa atcagttttc gcgtcgcgca cgcggcgtgt ccagggaata tgtttttcga
     4141 tcatctccag ttcctgcgcc gtaaataaat atgcgtattt tttatcggac aaaatggcga
     4201 agctggcttt tttgttcgga attttcgtgc gaaacgaatt taacatacat acttttccgg
     4261 cgcggtatgc attcgacagc ggattgtctt cgggaaattt ttccagaaac tcctgaatca
     4321 cgactctttt ataaaaaata tctatttcaa acgcgccgac gcgcaaaacc cgcccgtcga
     4381 attcgagttc gtgcgggtcg gcgatgatgc tttcgtgacc ttcggattga aaaaattccc
     4441 gcaaaaccac aaattccgaa taagtcgaaa cgccgttcca atcgacgatg gcaatgttcg
     4501 gtttttcctt tttcccgccg aaatcgcggt aatccgaaac gagcgccgcc agcagtttga
     4561 catgcggttt cggtcgccgg tgtttgtgtt cgcttaaaaa ctcgcgcact tccggaatca
     4621 attccataac cttttcgatt tgcatctggt cgatgattcc ggcgggcgtt tcaccgttgt
     4681 attccaaaaa cttgaaatct tcgccgtgcg cgaaggcatc gaaccggctc gaattgcaaa
     4741 cggttttgta gcgcggatcg attcgcgcca gacgctcctc gcgttcggtc aaatccaatt
     4801 cgcgcatgat ttcttcgttt tccagcgcgc ccagcgtcat tttttcgagc gcgccgccta
     4861 agacttccgc cgcgctcgta attttttcat aaatttttcg gctgaaaaac gccgggcgca
     4921 ggaaagggca aaacggtcgc tcgcctgata gaattccgag ctcgcgctgg gcttcatcca
     4981 gctcggcgaa aagctttttg tcaaagcgat gattggttaa aatccgctcg tcgagagttc
     5041 ggacggcttc ggcaagttta tcgttcataa tagaatcaaa tgtatccgag tcgccgcgca
     5101 aaacctaatt cggcagcttt ttcgcgaatt tctcaatatt ccgtgcaggc tattccataa
     5161 ttcgattttc ctgatatact tgtatgcgaa aatgcaactt tggctgatgg gttatcgtaa
     5221 gcagtttggc aatcgttctt ttatttgcaa atttatcagg agctagatat taaaaatggg
     5281 attgtgggca agaattacga gaatatttcg cgcgagcacg ggcgcggctt tggacaaaat
     5341 ggaaaatccc gaattggttc ttcagcagac gattcgggat atgcgcgacc gcgtgccgga
     5401 gctgaataat tccgtcgcgc aggtgatggc gaccgaacgg cttttggcga aaaacaaaga
     5461 aaatctggcg aaccaggtcg tcgacctcga ttcgaaaatc aaggcgtcgg tcaaaatggg
     5521 acgcgacgat attgcgacgg cttacatc
//
LOCUS       DASWNE010000445         2329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_367423, whole genome shotgun sequence.
ACCESSION   DASWNE010000445 DASWNE010000000
VERSION     DASWNE010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367423"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            613..1782
                     /locus_tag="VGB68_08940"
     CDS             613..1782
                     /locus_tag="VGB68_08940"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0049396.1"
                     /translation="MNENNLEAAVRVLREAIREACRNLIERETLVELVALAAVAQEHV
                     LIVGAPGTAKSEAVRRIAKATGGNYFEYLLGRFTEPSEIFGPVDLRKLKEGIVETETT
                     GMLPEAEIAFLDEIFLGSTAILNTLLGILNERIFRRGHTRMNCPLRVCVGASNALPED
                     ESLAAFADRFLVRLFVEPIPDPLLENLLEGGWDLAKREFSHTANIKDIDFLSEMAGGV
                     KLETVRPQIAQAVRLLRNAGINISDRRAVKMQKLVASAAILDGRLEARNSDLWTLVYA
                     IPTSEQQSLSRDILHELMAQTENRTLPFAAEEASMGMKARATRIANLSQEMLENRPAE
                     TENLPAWKLKLEGIAREIDAGFNAETIPPDLAEVRARLVTILENGARELERDAAV"
     gene            1779..2204
                     /locus_tag="VGB68_08945"
     CDS             1779..2204
                     /locus_tag="VGB68_08945"
                     /inference="COORDINATES: protein motif:HMM:NF040397.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049397.1"
                     /translation="MNAGLLKIHWQNRPDALSPCAVVAFDGAAIYLAEKLLSFEDEKL
                     HFLQGISGKQMILLTGESENLPWTNGAIYLGRDAQIPSVLLPTTLKPNCPLDLFERAL
                     NEKFNRFTPFAVLPEKIIPFGAAKPLSRAVLENWLSENR"
BASE COUNT          636 a          539 c          562 g          592 t
ORIGIN      
        1 ggacttgcgc gataaagcca gaagctgagc ctgatttaac ggaatcaggc tcagcttcta
       61 gctttatcgc gtttatgtaa gatttatcac aaatggacgt gtctcttaat ggaacgatat
      121 aatcgccaaa aaaccgcaaa aaccggctga aaaattatta caaatacgaa agaagctggg
      181 gctttctcaa aatgagctga tacgtcattt gagtgttttt cggggaggag ccaagcgaca
      241 aaatttcata aacacctcca tttacggctt agggaaaaaa tcgggtaact ccaactatct
      301 tcctgcgccg taattatttt acgtagcatt attggttagt aaagtcagct tattgagaga
      361 aattttcact ttatgcgcta aaaatagcag aaattttcac tttatgcgct acaccttttt
      421 cactttatgc gcgattttat tttccagatt gttgatttta ttaaaatgcg attttcaatt
      481 tatgggcgat ctcacagaaa gttaactaat ggtttttcgt ttttcagaaa ttatgtatat
      541 tttaagaagg tgtttattca ctacaagcag aactcgtttt aaggtctcac gaatattaaa
      601 tttatcaatc aaatgaacga aaacaatctg gaagcagcag tcagggtttt acgcgaagcc
      661 atacgggaag cgtgccgcaa tctaatcgag cgggaaacct tagtcgagct cgtcgcgctt
      721 gccgcagtag cgcaggagca cgtgctcatc gtcggcgcgc ccggaaccgc aaaaagcgaa
      781 gccgtcaggc gaattgccaa ggcgaccggc ggaaactact ttgagtatct cttagggcgc
      841 tttacggagc cgagtgagat tttcggtccg gtagatttac gcaaattgaa agaaggtatt
      901 gtcgaaactg aaaccacagg gatgttgccc gaagccgaga ttgcctttct ggatgagatt
      961 tttctaggct cgacggcaat attaaataca cttctgggaa ttttaaatga gcgtatcttt
     1021 cggcgcggac atacccgaat gaactgtcct ttacgcgtgt gtgtcggcgc ttcaaacgcc
     1081 ttgccggaag atgaatcttt agcggcattt gcagaccgct tcctggtgcg gcttttcgtc
     1141 gaaccgattc ctgacccatt gcttgaaaat ctgttggaag gcggctggga tttagccaaa
     1201 agagagtttt cgcacacggc aaacataaaa gatatcgact ttctctcgga aatggcgggc
     1261 ggcgtaaaac ttgaaaccgt tcgtccgcaa attgctcaag ccgttcgcct tttgcgaaat
     1321 gccggaatca acatctcaga ccgccgggca gtcaaaatgc agaaattagt cgcttcggcg
     1381 gcgattcttg acggaagact ggaagcccga aacagcgatt tatggacgct cgtttatgct
     1441 attccgacaa gcgaacagca atctctgtca agagacattc tgcacgagct gatggctcaa
     1501 accgaaaatc gaacgctgcc gtttgccgcc gaagaagcct ccatgggaat gaaagcgcgg
     1561 gcgacacgca tcgccaatct atcgcaggaa atgctggaaa accgtccggc ggagacggaa
     1621 aatttgccgg cgtggaaact caaactcgaa ggcatcgcgc gcgaaatcga cgccggcttt
     1681 aacgccgaaa cgattccgcc ggatttagcc gaagttcgcg ctcgattagt tacgattctc
     1741 gaaaacggcg cgcgagaatt agaacgagat gctgccgtat gaatgccggc ttactaaaaa
     1801 ttcattggca aaaccgccct gatgcgcttt ctccctgtgc agtggtcgcc tttgacggcg
     1861 cggcaattta cttggcggaa aagcttctgt cgttcgagga cgagaaattg cattttcttc
     1921 agggaatcag cggcaaacaa atgatattac taacgggcga gagcgaaaat ctgccctgga
     1981 cgaacggcgc gatttatctt ggacgcgacg cgcaaatacc ttcggttctg ctgccgacga
     2041 ccttaaaacc aaactgcccg ctcgatttat tcgagcgcgc cttaaatgaa aaattcaata
     2101 gatttactcc ttttgccgtt ctgccggaaa aaattattcc tttcggtgcg gcaaaaccct
     2161 tatcgcgagc ggtactggaa aactggcttt ctgaaaatcg atgaagttac ctgctagtct
     2221 cgcgccctgg gcgcagcaac ttaaaatctt tcccgatgaa atcagccttg cgcttggggc
     2281 tatggcgcaa aaaatttcgc cgtttgtcac tccgctgcgt tctcaggag
//
LOCUS       DASWNE010000446         2707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_398708, whole genome shotgun sequence.
ACCESSION   DASWNE010000446 DASWNE010000000
VERSION     DASWNE010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398708"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VGB68_08950"
     CDS             complement(<1..713)
                     /locus_tag="VGB68_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049398.1"
                     /translation="MKTFKLFGIILSIFCAACGDSSSTPDTYTTNGGGYITTTSTGTT
                     INTTAPAVSNIATAESGLDLQAVAGLAKKAKNAEDFERLLNQPDGVNNLDLDEDGKVD
                     YINVTEFKDGTKNAKGYSLSVDLGEDDIQEIATIQLERDHPNDKTIDVYLAGNQEIYG
                     TDDYYAGSWDYPDSTFAAWSFGSRPYYSSTYRRGYYPSYYSSFAVVGLSSYRSKMQSY
                     RAASPFKSIATVLTKAKLTS"
     gene            complement(789..1688)
                     /gene="era"
                     /locus_tag="VGB68_08955"
     CDS             complement(789..1688)
                     /gene="era"
                     /locus_tag="VGB68_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEY0049399.1"
                     /translation="MSSENYRSGYVGLIGRPNAGKSTLLNHLVGEKIAAVSNKPQTTR
                     HKIQGIVTSEEGQIVFVDTPGVHKPGYLLNRRMMAAVHDAILSVDVLVLLRDASVSTG
                     NGDKFVLDLIKQADKPTVLVLNKIDKIKEKSALLPLIESYANEFDFAEIVPISALKGE
                     AIDNLLSQITKHLPQGEPIFGEDELTDQSMRSIVAEMVREKILETTGEEIPYVTAVIT
                     EKYDETDPTLTRIYCAIYVERSSQKAIIIGKQGAKLRQIGTRARLDMEKLLGTRVYLQ
                     LFVKVVEDWRNSEHQLNEIGIEE"
     gene            complement(1812..>2707)
                     /locus_tag="VGB68_08960"
     CDS             complement(1812..>2707)
                     /locus_tag="VGB68_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049400.1"
                     /translation="EIKPTPSPSPTVEPSPEPSPTPEIKAEATPEIKTERSPEVKTTP
                     ETPVEPAPETVAKVETTPKPPTPTTPERIIVEVTDPTAKTTVETKTEPAPNPAPGNVK
                     SEKTVVEITDPLEKKRVETKTQATTDKPLFDPIVISVPKNETTKSKTEPAEEKPSEAP
                     QTEEPKTETQPKSTEEKPKTGDPSDNGEQRVRVIVTDNLPGENPPCSLVAGQTSISLL
                     NSGGNLGVLVGFDDNRGDISKITAISSSPDDIEVTVEPEIGKQSRRAFFIIKSISAKT
                     GIFTVTFNSPCGKKEIQVKVR"
BASE COUNT          539 a          674 c          701 g          793 t
ORIGIN      
        1 gaggtcagtt tggctttcgt caaaaccgtg gcgattgatt taaacggcga cgccgcgcga
       61 tagctttgca ttttcgagcg gtaactgctc aagccgacga ccgcgaacga cgaataatac
      121 gaaggataat aaccgcgccg atacgtcgaa gaataatacg ggcgcgagcc gaacgaccac
      181 gcggcaaacg tcgagtcggg ataatcccag cttccggcgt aataatcgtc cgtgccgtaa
      241 atttcctggt ttccggcgag gtaaacgtcg attgttttat cgttcggatg gtcgcgttcg
      301 agctggatgg tcgcgatttc ctggatgtcg tcttcgccca aatcgaccga cagcgaatat
      361 cctttcgcgt ttttcgtgcc gtctttgaat tcggtgacgt tgatgtaatc aaccttgccg
      421 tcttcgtcta aatccagatt atttacgccg tccggctgat tcaaaagacg ctcgaaatct
      481 tcggcgtttt tcgctttttt agctaatccg gcgaccgcct gcaaatccag accgctttcc
      541 gccgtcgcga tatttgaaac ggcgggagcg gtcgtattta tcgttgtgcc ggttgaagtc
      601 gtcgtaatgt aaccgccgcc gttcgtcgtg taagtgtcgg gagtagagga actatcgccg
      661 cacgccgcgc agaaaatcga cagtatgatt ccgaaaagtt taaaggtttt cattttttgc
      721 agctccgaat tgattttttg ccctcaatct tagcagcttg ttcgaatggc gacaaatctt
      781 ttttcggatt attcctcaat gccgatttcg ttcagctggt gctcgctgtt gcgccagtct
      841 tcgacgactt tgacaaacag ttgcaggtaa acgcgcgttc ccagcagttt ttccatatcg
      901 agccgcgcgc gagtgccgat ttgccgcagt tttgcgcctt gtttgccgat aataatcgct
      961 ttttgcgacg agcgctcgac gtaaatcgcg cagtaaattc gggttaaagt cgggtcggtt
     1021 tcgtcgtact tttcggttat gaccgccgtc acgtatggaa tttcttcgcc cgtcgtctcc
     1081 aggatttttt cgcgcaccat ttcggcgacg attgagcgca ttgattggtc ggtcagctcg
     1141 tcctcaccga aaatcggctc gccttggggc agatgtttcg tgatttggct caataaatta
     1201 tcaatcgcct cgcctttgag cgcggaaatc ggcacgattt cggcaaaatc gaattcgttc
     1261 gcgtaagatt cgataagcgg cagaagcgcg cttttttctt tgattttgtc gattttattc
     1321 aggacgagaa ccgtcggctt atcagcctgt ttgattaaat cgagcacgaa tttgtcgccg
     1381 tttccggtcg aaacggaagc gtcgcgcaaa agcaccagaa catcgaccga aagaatcgcg
     1441 tcgtgtaccg ccgccatcat ccggcggttt aataaatatc cgggtttgtg cacgcccggc
     1501 gtatcgacaa aaacgatttg tccttcttcg ctcgtgacaa tgccttgaat tttatgccgc
     1561 gtcgtttgcg gtttgttcga gacggcggcg attttctcgc cgaccagatg atttaaaagc
     1621 gtggattttc ccgcgttcgg gcgaccgatt aaaccgacat agccgcttcg gtaattttct
     1681 gaactcataa gataaattta ccataccaac gccgtcgaca cggaaaatat caagccgaaa
     1741 gtaaaaaggc aaaaggcaaa attgaaaaac cgattgcttt ttgccttttg ccttttacct
     1801 tttgcgttga tttatcttac tttcacctga atttcctttt tgccgcacgg cgaattgaaa
     1861 gtcactgtaa aaattccggt tttcgcgctg attgatttaa taatgaaaaa ggcgcgccgc
     1921 gattgtttgc cgatttccgg ttcgaccgtc acttcgatgt catcgggact gctggaaatc
     1981 gcggtgattt ttgaaatatc gccgcgattg tcgtcgaaac cgaccagcac gcctaaattt
     2041 ccgccgctgt ttaaaagcga aatgctggtt tgacccgcaa ccagcgaaca cggaggattt
     2101 tcgcccggca ggttatccgt cacgataacc cgcacgcgct gttcgccgtt atcgctcggg
     2161 tcgccggttt tcggtttttc ctccgtcgat ttcggctgtg tttcggtttt gggttcttcg
     2221 gtttgaggcg cttcagacgg cttttcttcc gccggttcgg tcttcgattt ggtcgtctcg
     2281 tttttcggga cgcttattac aatcgggtcg aacagaggct tgtcggtagt agcctgagtc
     2341 tttgtttcaa cgcgtttctt ttccagcggg tcggtaattt cgaccaccgt tttttcagat
     2401 tttacattgc ccggcgccgg gttgggagca ggctcggttt ttgtttcgac agtcgttttt
     2461 gcggtcgggt cggtaacttc gacaataatt ctttcgggcg ttgtcggcgt cggcggcttt
     2521 ggcgtcgttt caactttcgc taccgtttcc ggcgcaggct cgaccggagt ttcaggtgtc
     2581 gtcttaactt cgggcgaccg ctctgttttt atttcgggag tggcttcggc ttttatttcc
     2641 ggcgtcggcg aaggttcggg cgacggctcg acggtcggag acggactcgg cgtcggtttt
     2701 atttccg
//
LOCUS       DASWNE010000447         1836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_120489, whole genome shotgun sequence.
ACCESSION   DASWNE010000447 DASWNE010000000
VERSION     DASWNE010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120489"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(322..1713)
                     /locus_tag="VGB68_08965"
     CDS             complement(322..1713)
                     /locus_tag="VGB68_08965"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY0049401.1"
                     /translation="MKKFIYTIAIVFAFSFNLSAQMYNDATLAKVREQDAASRDAAGR
                     LLTLTAAEHNYRADVYSTNRYFKEAREHWQKVLDHYRADTALMPKALFGIARSFMWER
                     QYDKAVAHFDLLTRDYLNTKEGREGLAFKGASYVRMSRHNEAAKTYEQYTIMFPTGEK
                     IESSYLNIIDALREAKRYDEANAWVEKARRRFMGMPTEINALHARLRMEIHRGEWNRA
                     ILAADTLRDLGNFRSSMTSLDEVNYLKAVALEKAGRRPEAVAVYSTIPDALTSYYGGL
                     ATEKLKVLSPSAPRVAKTASVSSSLVKDFPAPYRAELLRYARPKGIDPRFMLAIMKQE
                     SSFRTNAKSPAAARGLLQLVFDTALKYNKQAGFPNLQAEDLYRPDVNIAIASIYIEDL
                     LDQFDNLYEAVAASYNGGEDNAARWLARSKPNEAGIFATEVGFAESKNYVYKVMNNYR
                     IYRELYTEDLQRR"
BASE COUNT          401 a          430 c          536 g          469 t
ORIGIN      
        1 aaaaaggcag ccggttcggt agaagttaat cttactaaag acggggcaat gtttaagact
       61 gatgatagag agaagtaaaa ttaaaggagt ctttggcttt atcgcttctc gttattaatc
      121 aggcaaggca gaacaaatca atggacgcga gggcgcgggc agcgactttg ttatgaacgt
      181 ccattgatga actcagtact gcgtgtttat gccttcgtta cctgttgaaa tgataaaagc
      241 cggaatgttc cggctttttg ttttgttgaa tgtttgatgt aaaaaatgcg cccaagccgg
      301 acgcacgggc ttctgccttt tttatctgcg ctgtaagtct tcggtgtaaa gctcgcggta
      361 gattctgtaa ttgttcatta ctttgtagac gtagtttttc gattcggcga agccgacttc
      421 cgtggcgaag attccggctt cgttgggttt cgagcgggcg agccagcggg cggcgttgtc
      481 ttcgccgccg ttgtaggaag cggcgacggc ttcgtagagg ttgtcgaatt ggtcgagcaa
      541 gtcttcgatg tagatgctgg cgatggcgat gttgacgtcg ggacggtata aatcttcggc
      601 ttgcaggttg ggaaaaccgg cttgcttgtt gtatttcagg gcggtgtcga agacgagttg
      661 gagcaagccg cgagccgccg ccggagattt ggcgttcgtg cgaaagctgc tttcctgttt
      721 cataatggcg agcataaagc gcgggtcgat gcctttcggg cgggcgtagc gcaaaagttc
      781 ggcgcgatag ggcgcgggaa aatctttgac gagcgacgag gaaacgctcg cggttttggc
      841 gacgcgcggg gcggacgggc ttaaaacttt taatttttcc gtcgccaagc cgccgtaata
      901 cgaggtcaga gcgtcgggaa tcgtcgaata gacggcgacg gcttcggggc gtctgcccgc
      961 tttttccagc gcgacggctt tcaggtaatt cacctcgtca agactcgtca tcgagctgcg
     1021 aaaatttccc aaatcgcgca gagtgtccgc cgccagaatg gctctgttcc actcgccgcg
     1081 atgaatttcc attctcaagc gcgcgtgcag ggcgttgatt tcggtcggca ttcccataaa
     1141 tctccggcgc gctttttcca cccaggcgtt cgcctcgtcg tatcttttcg cctcgcgcag
     1201 cgcgtcgatg atgttcagat acgagctttc gattttttcg ccggtcggaa acattatcgt
     1261 gtattgctcg taggttttcg ccgcttcgtt gtgcctgctc attcgcacgt aggacgcgcc
     1321 tttaaacgcc aagccttcgc gaccttcttt cgtgttcagg taatctctgg tgagcaaatc
     1381 gaaatgagcg accgctttgt cgtactggcg ctcccacata aaggagcgcg cgatgccgaa
     1441 aagcgctttc ggcattaagg ctgtgtccgc gcggtaatgg tcgagaactt tctgccagtg
     1501 ctcgcgggct tctttaaaat aacgattggt cgagtaaacg tcggcgcgat aattatgctc
     1561 cgcggcggtc agcgtcagca gcctgccggc ggcgtcgcgg ctcgcggcgt cctgctcgcg
     1621 gactttggcg agcgtcgcgt cgttgtacat ctgcgccgat aaattgaagg aaaaagcgaa
     1681 aacgatggcg attgtgtaaa taaatttttt catgattgca aagcggaaac tcaagggaga
     1741 tatagaataa ccgattttta gatgcgagaa aagccgttcg gtaaaccggc taaaaattac
     1801 gcgcccgaaa aaaatcgtta aaaggaaaaa gcacgg
//
LOCUS       DASWNE010000448         4097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_545879, whole genome shotgun sequence.
ACCESSION   DASWNE010000448 DASWNE010000000
VERSION     DASWNE010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545879"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VGB68_08970"
     CDS             complement(<1..732)
                     /locus_tag="VGB68_08970"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0049402.1"
                     /translation="MNFKQFYLGCLAHASYYIGSGGEAAVIDPQRDVSQYLAEAEANG
                     QKIKYVFETHLHADFVSGHNELARRTGAKIVFGARAKAEFEHLAVKDGDELTIGNVRL
                     GILETPGHTPEGISILVEDAEKPGEPKKILTGDTLFIGDVGRPDLAGGAGFTAEQMAE
                     MLYESLHEKLLTLADDTEVYPAHGAGSLCGKNMSKETWSTIGQQRKLNYALQPMTKAD
                     FVRMMTAELPEAPAYFPLSVRRNRAG"
     gene            complement(1163..1978)
                     /locus_tag="VGB68_08975"
     CDS             complement(1163..1978)
                     /locus_tag="VGB68_08975"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0049403.1"
                     /translation="MPKIVIVEDNTLIAKLYENKLSAEGHAVKVAADGASGFELIKEF
                     KPDAVLLDLMLPGMSGIEIIKELRRNWEFTNVPILAYSGGDNDLLDAATEAGTTYIVS
                     KNENSLKEILAHLNDLLALTQNWQIYNSSAYGYESEPAATEKTGAASGERVLIVEDDL
                     LIVTVVKDIVEKAGYQPVVAGDGREAYRILATDANFAAGIFDVHVPYIEGTDLVRHMR
                     TEKRLMKIPVIIMTSEESVKIQLDSLSAGASVFLPKPFERKTFESMFKALVGE"
     gene            complement(1989..>4097)
                     /locus_tag="VGB68_08980"
     CDS             complement(1989..>4097)
                     /locus_tag="VGB68_08980"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0049404.1"
                     /translation="RADGTPHFIMGTGQDVSDLKEARDEALKSARLKSEFLANMSHEI
                     RTPMNGVIGMTGLLLDTALDAEQRKFAGLIRSSGEALLTIINDILDFSKIEAGKLDFE
                     ALDFDVRETVESTIELFAGRARERGNEITSLIYTDVPLGLRGDAGRLRQVLSNLAGNA
                     VKFTKNGEVIVRVKRESESASHVTLHFSVSDSGIGVSTEMQSQLFQPFTQADATTTRR
                     FGGTGLGLAISRRLVEMMGGEIGVRSEAGEGATFWFTAKFEKQTRVQLTPAGGSQNHL
                     AGLRVLIVDDNAVNREVLVYQTRSWKMTAKEADSGAYALKMLSRAAAEAESFDLVILD
                     LQMPEMNGLELAEKISALSLAVQPKLIMLSSNGRRGEAEKARSAGVHGYLSKPYRQEE
                     LLNCLLGIIGNKDASPPKQMVTRFNTGETKPENARESAERRNMHASVSAASRRRILVV
                     EDNAVNQTVAKTQLEKFGYRADVAANGLEALDALLRIPYDLVLMDCQMPEMDGYEATA
                     AIRRREQGTALRTPIIAMTAHAIDGEREKCLAAGMDDYISKPVQKDVLRQTVERWLNT
                     DAAPVTKTGGEVAGDKAPDDVCVVDLELLKDLTLGDAAMLREIIELYVQQTGEQLDEI
                     SRAITAQNPEMLYQISHKALGGSATCGMKTIVPFFKELEQSGKMRQFENAEALLSGAR
                     RSFIEISRECEEIVREPVSN"
BASE COUNT          891 a         1145 c         1028 g         1033 t
ORIGIN      
        1 gcccgcgcgg tttcgccgca cgctcaacgg aaaataagcc ggcgcttcgg gcagctcggc
       61 ggtcatcatc cggacgaaat ccgctttcgt catcggctgc agagcgtagt ttaattttcg
      121 ctgctgaccg atggtcgacc acgtttcctt cgacatattt ttgccgcaca ggctgcccgc
      181 gccgtgcgcc ggataaactt ccgtatcgtc ggcgagcgtt aaaagctttt cgtgcagact
      241 ttcataaagc atttccgcca tttgctcggc ggtaaagccc gcgccgcccg ccaaatccgg
      301 tcgcccgaca tcgccgataa aaagcgtatc gccggtcaga atctttttcg gctcgcccgg
      361 cttttccgcg tcctcgacca gaatcgagat tccttccggc gtgtgaccgg gcgtttcgag
      421 aattccgagc ctgacgtttc caatcgtcag ttcgtcgccg tctttcacgg ctaaatgctc
      481 gaattcggct ttcgcccgcg cgccgaaaac gattttcgcg cccgtccgac gggcaagctc
      541 attgtgaccg ctcacaaaat cggcgtgcag atgcgtttcg aaaacgtatt ttattttctg
      601 cccgttcgcc tcggcttccg ccagatattg cgacacgtcg cgctgcgggt caatgaccgc
      661 cgcttcgccg cccgagccga tgtaatacga cgcgtgagcc agacaaccga gataaaattg
      721 tttgaaattc ataatgcccc ttctgacaaa aaaagctgcg gaaacttgtc ttgtttccac
      781 aactttatat caattttccg ggttagaaaa gccgcccgga ttttgacgag cggcgacgat
      841 gactttatta aaatcgcgcg caacccttat agcgtaataa taaagtcgtg tactttacta
      901 tccataagtt attgattccg cgtgaaaagc taatcacgaa gcgaataata aaattgtata
      961 aaaggaaatg tcttaaatat ttgtactgaa aaattacccg attttgtaat aataaagtcg
     1021 tatacaaatg cgacctgccc ttttaaagaa aacgttaaaa ttcttgtaaa tgaagaaaat
     1081 cgattgaaat ttgaagcata agcttgccgt ttcaaaacag ctcgcggcaa gcttatgttt
     1141 ccgtttccgg agccgcaaat tcttattcgc cgacgagagc cttgaacata gattcgaagg
     1201 ttttgcgctc gaacggcttc ggaaggaaga cggaagctcc ggcggaaaga ctgtcgagct
     1261 ggattttgac gctttcttcg gaggtcatta ttatcaccgg aattttcatt aaacgttttt
     1321 ccgtgcgcat atgtctaacc aaatcagtgc cttcgatata aggcacgtgc acgtcgaaaa
     1381 ttcccgccgc aaagttggcg tcggtcgcca ggatgcggta agcttcgcgc ccgtcgccgg
     1441 cgacgaccgg ctggtaaccg gctttttcga caatatcctt gacgaccgtc acaatcaata
     1501 aatcgtcttc gacaattaaa acgcgttcgc cggatgccgc gccggttttt tcggtcgcgg
     1561 caggctcgct ttcgtatccg taggcggaag aattgtaaat ctgccagttt tgcgtcagcg
     1621 cgagcaagtc attcagatga gccagaatct ctttcagaga gttctcgttt ttagaaacga
     1681 tgtatgtcgt gccggcttcg gtggcagcgt cgagcaaatc gttatcgccg cccgaataag
     1741 cgagtatcgg gacattggta aactcccagt ttcggcgcaa ttctttaatg atttcgatgc
     1801 cggacattcc gggcagcatc aaatcgagca aaaccgcgtc gggtttaaac tctttgatta
     1861 attcgaagcc ggaagcgccg tcagcggcga ccttaaccgc gtgtccttcg gctgaaagct
     1921 tgttttcata taatttggca atgagcgtgt tgtcttcgac aataactatt ttgggcattg
     1981 attctccttt aattcgagac gggttctctg actatttctt cgcattcgcg gctgatttcg
     2041 ataaaagacc ggcgcgcgcc ggacaataaa gcttccgcgt tttcaaactg ccgcatttta
     2101 cctgattgtt cgagttcttt aaaaaacggg acgatggtct tcatcccgca ggtggcgctg
     2161 ccgcctaatg ccttgtgtga tatctgatac agcatttccg gattctgcgc ggttatcgcc
     2221 cggcttattt catcgagctg ttcgccggtt tgctgcacgt aaagctcgat tatttcgcgc
     2281 agcatagccg catcgccgag cgtcaaatct tttagcagct cgaggtcgac gacgcagaca
     2341 tcgtccggcg ctttatcgcc cgcgacttcg ccgccggttt tcgttaccgg cgcagcatcc
     2401 gtatttagcc atctttcgac tgtttgccgc aaaacgtcct tttgaaccgg cttgctgata
     2461 taatcgtcca ttccggcggc gaggcatttt tctctttccc cgtcgatggc gtgcgccgtc
     2521 atcgcaataa tcggcgtgcg aagcgccgtt ccctgctcgc gccgtcgaat ggcggcggtt
     2581 gcctcgtatc cgtccatctc cggcatctgg caatccatca gcaccaaatc ataaggaatg
     2641 cgcagcagcg cgtcgagcgc ttccagcccg ttggcggcga catcggcgcg ataaccgaat
     2701 ttttccagct gcgttttggc aaccgtctga ttgaccgcat tatcttcgac cactaaaatt
     2761 ctccggcgcg acgcagccga aaccgaagcg tgcatatttc ggcgttcggc gctctcgcgt
     2821 gcattttcag gcttagtttc gccggtattg aatcgcgtga ccatctgttt cggcggcgaa
     2881 gcatccttgt tgccgatgat gccgagcagg caattcagaa gctcctcctg ccggtaaggc
     2941 tttgataaat aaccgtgaac accggcgctt cgggctttct ccgcttcgcc ccgccgcccg
     3001 tttgacgaca gcataatcaa tttcggctga acggctaacg aaagcgcact aattttttcg
     3061 gcaagctcca acccgttcat ttccggcatt tgcaaatcaa gaataacaag atcgaatgat
     3121 tcggcttcgg cggcggcgcg cgaaagcatt ttaagcgcat aagctcccga atccgcttct
     3181 ttcgccgtca ttttccagga acgcgtctga tacacgagaa cttcgcggtt caccgcgttg
     3241 tcatcgacaa tcagaacccg caatccagcc agatggtttt ggctgccgcc ggcgggcgtt
     3301 aattgaacgc gagtctgttt ctcgaattta gccgtaaacc agaaagtcgc gccttcgccc
     3361 gcttcgcttc tgactccgat ttcgccgccc atcatttcga ccagccggcg tgaaatcgcc
     3421 aagcctaagc cggttccgcc gaaacgccga gtggttgtgg catcggcttg cgtaaaaggc
     3481 tggaaaagct gcgactgcat ctcggtcgaa actccgatgc cggaatcgct gaccgaaaaa
     3541 tgaagcgtta cgtgcgacgc ggattcgctc tcgcgcttga cccgaactat gacttcgccg
     3601 tttttcgtga acttgacggc gtttccggcg agattcgata aaacctgccg caggcgtccg
     3661 gcgtcgccgc gcaagccgag cggaacgtcc gtgtaaataa gcgacgtaat ctcgtttccg
     3721 cgctcgcgcg cgcgcccggc aaataattcg atggtgcttt ccaccgtttc gcgcacatca
     3781 aaatccagcg cttcgaaatc gagcttgccg gcttcgattt tggaaaagtc caggatgtcg
     3841 ttgataatcg tcagcagcgc ttcgccgctg gagcgaatca gcccggcaaa cttgcgctgc
     3901 tcggcgtcca gcgcggtatc gagcagcaag cccgtcatcc cgatgacgcc gttcatcggc
     3961 gtgcgaatct cgtgactcat attggcgaga aattccgatt tgagccgcgc cgatttcagc
     4021 gcctcgtcgc gagcttcttt gaggtcggag acgtcctgtc ccgttcccat aatgaaatgc
     4081 ggagttccgt cggcgcg
//
LOCUS       DASWNE010000449        12668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_580349, whole genome shotgun sequence.
ACCESSION   DASWNE010000449 DASWNE010000000
VERSION     DASWNE010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12668
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580349"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VGB68_08985"
     CDS             complement(<1..180)
                     /locus_tag="VGB68_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049405.1"
                     /translation="MFIRRLDDGAELNLLQTRHAEQLFGLLKTDWEYFRQWMPNLHDD
                     YSVADSASFIHGSLNR"
     gene            complement(187..3138)
                     /gene="ileS"
                     /locus_tag="VGB68_08990"
     CDS             complement(187..3138)
                     /gene="ileS"
                     /locus_tag="VGB68_08990"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEY0049406.1"
                     /translation="MTEPLELKKTVNLPKTNFAQKANLGQSEPTRLKKWADAGLYKMI
                     SEARRGAQKFILHDGPPYANADIHIGTALNKILKDLVVKSRSMMGFDAPYVPGYDCHG
                     LPIETLVEKKLAEKGKNKADLPVATFRKICRDHASRAMDGQTRDFSRLGILGEWQTPY
                     LTMSHEYESETARLFGKFLERGYVYKGLRPVYWCVHDQTALAEAEVEYKLHTSPSIYV
                     KFPLINDAAELDENLAGRRVSLVIWTTTPWTIPANLGISVHPDFEYAAVEHGDEVLIV
                     ASELVGFVIVKCGIGGTNEEGERQLPPILAKFPGSRLKGMKAKHPWIERESLVMNGAH
                     VTLGGEADAETEIDVSDARNRSETSKAGTGLVHTAPGHGHDDFVIGKEYGLEIYCPVD
                     SAGRFTDEVEHFAGENIFKANPQIVELMRENNSLLFAENYEHRYPHCWRCKNPVIFRA
                     TPQWFISMDEAAKTGERKSLRAGALEQVEQVKWHPAWGAGRMTNMIKGRPDWCVSRQR
                     SWGVPIPVFYCQACDEAIVDSQIINHVADIFARETADAWYARPESELLPEGFACPKCN
                     SADFRKETDILDVWFDSGSSCVAVLETRGENLRFPADVYLEGGDQYRGWFNSSLMCGL
                     ASHDTAPYKTVITHGWVVDGEGKKQSKSIGNVTAPIEIINQSGADVLRLWAAAVDYTE
                     DVRCSDEILQRVTDAYRKFRNTLRYALGNLDGFNPETESVEKPILQYAGGAEPGEIEN
                     LFEIDQWALANLDEVTARVLEAYENYDFQAVYQTLYNFVTVTLSARYFDIIKDRLYIF
                     APNSLERRSAQTALYQITDKLCRLLAPIMVFTADEAFENLPNQRLASVHLAEFPKVSG
                     ADNAQLLADWERIFAMRDEVLKALEEARNAKQIGSSLEAKVVLTTDKETTRFLIPYYE
                     DLRYIFIVSQVEVHEGEAFGVKIQKADGAKCERCWNYSVRVGESEKYPTVCERCLAAL
                     TEIEKTEGATV"
     gene            3544..4695
                     /locus_tag="VGB68_08995"
     CDS             3544..4695
                     /locus_tag="VGB68_08995"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0049407.1"
                     /translation="MKLNLKRCSSVLGLIILFLLPVFSQQPTPTPAAEEPIKILTEEV
                     LLNVTAQTVKGKFVPTLKPDDLLIVESGTPQKIESMKKVPASVLLLLDTGGNLNFAKS
                     LNMTRLTAKLLVEKISPGNTLGVMQSYDKIETVSGWTENREKVQGDLDKKLFSGNRSQ
                     FSGSINAAIEMFKTRPPENRHIVFIGDGLDTLAGVNERRKALENLLAANITVHVIAYN
                     KMEAKRAKPMTRRIQIGEEIETPRLPEHQLEIIIEGLPTQMRDGFRRMAKAERLFIIR
                     LDNQNLKLAKQKLEQWNKSQTEMQTLAEDTGGMFQAPEELETMWSFAQEIAQAIDSQY
                     VVTYIPTKSFAEADGSGVEARKIRVSTHCDGVIIRSRQKILSAARSVNR"
     gene            complement(5182..6534)
                     /locus_tag="VGB68_09000"
     CDS             complement(5182..6534)
                     /locus_tag="VGB68_09000"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEY0049408.1"
                     /translation="MNPNIFREYDIRGIVGEHLTDETVSLLAKAVGTFFRRNNAKSIA
                     LGFDARESSPRFRDLFVRGLNAAGCDVFDIGRVPTPVLYFAVFTREVDGGVMITGSHN
                     PPDHNGFKICLGKDALFGEQIQQIKEIAFNGKFAEGAGKVEALEVSGDYRREILARVS
                     LGNKKLKAVVDAGNGMGGVTAVPIYKELGIELIELFTEPDSNFPNHHPDPTVVENLLD
                     AIEAVKRSGADLGIAFDGDGDRIGVIDETGRIIWGDELMVLLSRSVLKANPGATIIAE
                     VKCSQNLFTDIEKHGGIPLMWKAGHSLIKSKMKETGAILAGEMSGHIFFADRFYGFDD
                     ATYAGARVLEILSKTDLSLSQMLSDLPAGVSTPEIRVDCPEEKKFVVVRQIAEEFSKT
                     NEVITIDGARILFAGGWGLVRASNTQAILVLRFEADSEENLRMIRELIESRVDYFIDA
                     "
     gene            6761..7753
                     /locus_tag="VGB68_09005"
     CDS             6761..7753
                     /locus_tag="VGB68_09005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0049409.1"
                     /translation="MKNLARFFGFSGLLLSFVLTVPAQTPRTSPTPPRGDDDGQVRTF
                     EVRLPVTVKDKKKNLVSGLKREDFLVFEDGVQQKVTFFSDEKTNPPVSVGVLMDTSPS
                     TAGKMKFSKEAAKNFIYTVTRIRKDQAAFMTFDHEINLLQDFTDKLDLLGKAIDGVKK
                     VGSQTALYDAIWQFTDEKLRNAPGRHVIVIITDGDDTFSRADINDAIDIAQRTETTIF
                     AISTKSGFLGSVPGVEAGTVKDEGDKLLDRLCRETGGEAFYSGDMLALERALDKVAKE
                     FRGQYIVTYRPANQEYDGRKRKVEVRFADKQKADDYRIRTKTEYRAVNDGLKKN"
     gene            7846..8958
                     /locus_tag="VGB68_09010"
     CDS             7846..8958
                     /locus_tag="VGB68_09010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0049410.1"
                     /translation="MSGCKNLTVLSIGLLFVASTLFGWNFSAAQQQQTTAPTQKQSVP
                     SPTATPGTEVVEEEDEPIKIDTELVNVLFTAQDNNRRLLTDLKKEDIRILEDGQPQQI
                     FTFARQTDLPLSIAIVIDTSQSQQRTLPEEKDAAKSFVESVVRPEKDEVAVLSFTGET
                     TLEQDLTGNVQRLRRAIDRVEFVPAAGTLNGVLIGTPPISGRSQQVAGSTAIWDALWV
                     TSNEVLSKAPERTRRAIVLMSDGVNTFGNKKLEDAVNQAIKNDAIIYSIGIGDYYYGG
                     IDESALRKISERTGGRAFFPRDEAELRKAFTQIQIEMRSQYLIAYEPSNQKRDGTFRK
                     IEIQVSNSELTKQKIRLTHRQGYFAKTASIQNKPAK"
     gene            complement(9437..12007)
                     /locus_tag="VGB68_09015"
     CDS             complement(9437..12007)
                     /locus_tag="VGB68_09015"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0049411.1"
                     /translation="MRYLFMLESGKNFGRYAVLSLIGKGGMGEVYLAQDKSLNRKVAL
                     KILSAKFADDYDLLRRFEREAETASALNHPNILTVYEFGKEDGLHFITTEFVEGETLR
                     EKLKRERLSLTEMLNIAEQAASALAAAHSAGVIHRDVKPENIMLRADSLVKVLDFGLA
                     KFIEKSEEHDPDAVTQPYVHTQPGTVMGTVFYMSPEQARGKQIDERSDVWSLGVILYE
                     MITARLPFEGETASDVIASILKSNLVPVTHYVSETPSELSSIIIKALRKNPAERYQKM
                     PEMLGEIKDFRQELEFEAKRLGKSGIVTQNNLQNGYRTQASLRPRQFATRIDAPDAPE
                     IPAPVKKYVPPALIIIPAAGLIALLVWQFGKTDGNFNPVSAVLIIALIAGGFLLYSTY
                     FRLGAGADYRSIAVLPLGNSGGDAEMEYLSEGISESLINNLSQLSDVKVIARSSSFKY
                     KYNAENLREIAKTLNVATILTGSVMRRGENLQISVELINARDNTQIWGEQYSRRADDL
                     LMIQSDISREIADKLHPRLTLSEQKQLVRHETKNSVAYELLLKGRFYRAKGGTESRKR
                     AVEYFNEAIAIDPNYALAYAELAFTYRILVGSGSLDPKEFTPKAEAAALKSLELDETL
                     ADAHFALAFLKMNGWEWSAAEFEFKRAIELNPNLARAYIGYSGYLSRTGRHDEAVREI
                     RRAKELDPLSTIINGNYGLVLYFAGRYDEAIKILRETLEFDPNFSFAHLYLGYNYSAK
                     KMFAEAIEAYRKAFSLGQETPSNKIYLGAAYAGAGEREKAQAILSELETSESYVSPAE
                     LAVLYTALEMREKAFASLEKAFALHDLQLQYLATDPAFKSLRNDSRFTDLLKKIGLPQ
                     "
     gene            12228..>12668
                     /locus_tag="VGB68_09020"
     CDS             12228..>12668
                     /locus_tag="VGB68_09020"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0049412.1"
                     /translation="MARINFCSRAVLVPALFALSFFISGCASSAGSRYFGKTQAPKDN
                     VLRYISGSEPESLDPHVSSGQPEARVYLAIYDGLVEYHPKDMHPIPAIAKSWEISPNV
                     DEFIFRLRADAKWSDGTPITARDFVYSFRRGFAPETASRTASLGY"
BASE COUNT         3089 a         3361 c         3156 g         3062 t
ORIGIN      
        1 acgattgagc gagccgtgaa taaaactcgc cgaatcggcg accgaataat cgtcgtgcag
       61 attgggcatc cattgacgga aatattccca atccgttttg agcaagccga ataattgttc
      121 ggcgtggcgc gtttgcagca gattcagttc cgcgccgtcg tcgagccgtc gaataaacat
      181 aagcttttag acggtcgcgc cttccgtttt ctcgatttcc gtgagcgccg ccagacatct
      241 ttcgcagacg gtcggatatt tttccgattc gccgactctg accgaataat tccagcagcg
      301 ctcgcatttc gcgccgtccg ctttttgaat cttgacgccg aacgcttcgc cttcgtgaac
      361 ttctacttgc gaaacgataa agatataacg caaatcctcg taatacggaa tcaggaaacg
      421 agtcgtttcc ttgtcggtcg tcaaaacgac tttcgcttcc aggctcgagc cgatttgttt
      481 cgcgttgcgc gcttcttcca gagcttttaa aacctcgtcg cgcatcgcga aaatccgttc
      541 ccaatcggct aaaagctgcg cgttgtccgc gccggaaact ttcggaaatt ccgccagatg
      601 aaccgatgcc aatctttgat tcggcagatt ttcaaacgct tcgtccgccg tgaaaaccat
      661 aatcggcgcg agcaggcgac acaatttatc cgtgatttga taaagcgcgg tctgcgcgga
      721 gcggcgttcc agcgagttcg gcgcgaagat atacagacgg tctttgatga tgtcgaaata
      781 ccgcgccgaa agcgttacgg tgacgaaatt ataaagcgtt tgataaacgg cttggaaatc
      841 gtaattttcg taagcctcca aaacccgcgc cgttacctcg tccagattcg ccagcgccca
      901 ttggtcgatt tcgaacagat tttctatctc gcccggctcc gcgccgcccg cgtattgcaa
      961 aatcggtttt tcgaccgatt cggtttcggg attaaagccg tcgagatttc cgagcgcgta
     1021 acgcaaggta tttctgaatt tgcgataagc gtccgtcacg cgctgcaaaa tttcgtccga
     1081 gcatcggacg tcttccgtgt aatcgaccgc cgccgcccag aggcgcagaa cgtccgcgcc
     1141 cgattgattg atgatttcaa tcggcgcggt cacgttgccg attgatttgc tttgcttttt
     1201 gccttcgccg tccacgaccc agccgtgcgt gataacggtt ttatacggcg cggtgtcgtg
     1261 cgaagccaag ccgcacatca acgaagaatt aaaccagccg cgatactggt cgccgccttc
     1321 cagatagacg tccgccggaa aacgcaggtt ttcgccgcgc gtttccagaa cggcgacgca
     1381 cgacgagccg gaatcgaacc agacgtcgag aatgtcggtt tccttgcgaa aatccgcgct
     1441 gttgcatttc gggcaggcaa agccttccgg caaaagctcg ctttccggtc gcgcatacca
     1501 cgcgtccgcc gtctctctgg caaaaatatc ggcgacgtga ttgattattt gcgaatcgac
     1561 aatcgcttcg tcgcacgcct gacagtaaaa aaccggaatc ggcacgcccc acgagcgctg
     1621 gcgcgaaacg caccaatccg gacgaccttt aatcatattc gtcatccgac ccgcgcccca
     1681 cgcgggatgc cattttacct gctcgacctg ctccagcgcg ccggcgcgca gcgatttccg
     1741 ctcgccggtt ttcgccgctt cgtccatcga gataaaccac tgcggcgtgg cgcgaaagat
     1801 aaccggattt ttgcagcgcc agcagtgcgg gtaacgatgc tcgtaatttt ccgcgaacaa
     1861 taaagaattg ttctcgcgca tcaattcgac aatctgcgga ttagccttaa aaatattttc
     1921 gcccgcaaaa tgctcgactt cgtcggtaaa tctgcccgcg ctgtcgaccg ggcaataaat
     1981 ttccaagccg tattccttgc cgataacaaa gtcgtcgtgc ccgtgtccgg gcgccgtatg
     2041 aaccaatccg gttccggctt tcgacgtctc cgagcggttt cgcgcgtcgg agacgtcgat
     2101 ttcggtttcc gcgtcggctt cgccgcctaa agttacgtgc gcgccgttca ttaccagcga
     2161 ttcgcgttct atccacggat gtttcgcttt cattcctttc agacgcgagc cgggaaattt
     2221 cgctaagatc ggcggcaatt ggcgctcgcc ttcctcgttc gtgccgccga tgccgcattt
     2281 gacgatgacg aaaccgacca attccgaggc gacgattaaa acctcgtcgc cgtgctcgac
     2341 cgccgcgtat tcaaaatccg gatgaacgga aatcccgaga ttcgccggaa tcgtccaggg
     2401 cgtcgtcgtc cagataacga gcgaaactcg tctgcccgcc agattttcat ccaattccgc
     2461 cgcgtcgttt ataagcggaa atttgacgta aatcgaaggc gaagtatgca gtttatattc
     2521 gacttccgcc tcagccagcg ccgtctggtc gtggacgcac cagtagacgg gacgcaagcc
     2581 tttataaacg tagccgcgtt ccagaaactt tccgaaaagc cgcgcggttt ccgattcgta
     2641 ttcgtgcgac atcgttaaat acggcgtctg ccattcgccc aaaattccga gccgcgaaaa
     2701 atcgcgcgtc tgcccgtcca tcgcgcgcga ggcgtggtcg cggcagattt tgcgaaacgt
     2761 ggcgacgggt aaatcggctt tgttcttgcc tttttcggcg agctttttct cgaccagcgt
     2821 ttcaatcggc aagccgtgac agtcgtagcc cggcacgtaa ggcgcgtcaa aacccatcat
     2881 cgagcgcgac ttgacgacca aatctttcag gattttgttg agcgccgtgc cgatgtgaat
     2941 gtcggcgttg gcatacggcg gtccgtcgtg cagaataaat ttctgcgcgc ctcgccgcgc
     3001 ttcggaaatc attttgtaaa gccccgcgtc cgcccatttt ttcaggcgcg tcggttccga
     3061 ttgaccgaga ttcgccttct gcgcgaaatt cgttttcggt aaatttacgg ttttttttag
     3121 ttctaaaggt tcagtcataa aaagttgtca gcggtcagcg gtcagcggtc agttgatgac
     3181 ttttgaacaa tgacggcaaa aataaaatct taacattcat tcaataaaaa atcccaaatc
     3241 ccaaatccgc gatcccaaat caaaaagtcc caagcctttt tcgcaagacc tgggactttc
     3301 gagcaaacgg ttacttccgg ctcaagctct ctcaaggagc aaagtccctt tcgctaataa
     3361 taattatgcc cgaagcaacc atcataaaac ttgttccgtc ttattttatg ccagtatttt
     3421 cgtcattagt caacaaaagg cgtaagtagc gcaaaactca aaataaaaag cgccgaatat
     3481 caggattccg aaattaaatt tattcctgcc tttggcgctt tgcattaata aacggttagc
     3541 tttatgaaat taaatctcaa aagatgctcg tctgttctcg gattaataat tttatttctt
     3601 ttgccggtgt tttcccagca gccgacaccg acgccggcgg cggaagagcc gataaaaatt
     3661 ctgaccgaag aagttttgct taacgtcacg gcgcagacgg tgaaagggaa atttgttccg
     3721 accctaaagc cggacgattt attaatcgtc gagagcggaa cgccgcagaa aatcgaaagt
     3781 atgaaaaaag ttcccgccag cgttttgctt ttgctcgaca cgggcggcaa tctgaatttc
     3841 gctaaaagtc tgaatatgac gcgcctgacg gcgaagcttc tggtcgaaaa aatttctccc
     3901 ggcaatacgc tcggcgtgat gcagtcttac gataaaatcg agacggtttc cggctggacg
     3961 gaaaatcgcg aaaaggtgca gggcgatttg gacaaaaagc ttttcagcgg caatcgctct
     4021 cagttttccg gctcgataaa cgcggcaatc gaaatgttta aaacgcgtcc gccggaaaat
     4081 cgtcatatag tctttatcgg cgacgggtta gacactctcg ccggcgttaa cgaacgccgg
     4141 aaagcgctgg aaaatctgct cgccgcaaac attaccgtcc acgttatcgc ctataacaaa
     4201 atggaagcga aacgcgcgaa accgatgacg cgccgcatcc aaatcggcga agaaatcgaa
     4261 acgccgcgcc tgcccgaaca tcagctcgaa ataattattg aaggtctgcc gacacagatg
     4321 cgtgacggtt tccgccgaat ggcgaaagcc gaacgcctgt ttatcatccg gctcgacaat
     4381 cagaatctga agctcgccaa acaaaagctc gaacagtgga acaaaagcca aacagagatg
     4441 cagacgcttg ccgaagatac gggcggaatg tttcaagcgc ccgaagaact ggaaacaatg
     4501 tggtctttcg cgcaggaaat cgcgcaggcg attgattcgc aatacgtggt gacttacatt
     4561 ccgaccaaat ctttcgccga agcggacggc agcggcgtcg aggcgcgaaa aattcgcgtc
     4621 agcactcatt gcgacggcgt cataatccgc tccagacaaa aaatcctttc cgccgcgcgg
     4681 tcggttaatc gttaaataaa cattttcagt ataataattg ccgccgaaag aatgtttccc
     4741 cacggctctg acgattgcag gtaagtttgc ggcagcccgg agcaggggaa aataaaaaca
     4801 gcataccgtt taaagtatgc tgtttttttg tttgttttac agcataccgt ttaaagtacg
     4861 ctgttttttt tgtttgtttt cgggtgcgac ctgttaattg ccggtcgccc cgttggtatt
     4921 atcatttatc atataatatc acccccagga cttacttaga tgcagctttt tagtcggagt
     4981 tgcctgacgg gaaattattt attgagaaat aatgaagctc gccgcttgcg taagcggcga
     5041 gcctctccaa gggaagataa ccagttgtcc gttctgtcct gtcaattaat agatagacgc
     5101 gggctgaaaa agtttttgcg aaaaaaatgt cagtaataaa aaaaagattg gcgagcattt
     5161 attttatttt gaaaccgttt tctaagcgtc gataaaataa tcgacgcggc tttcgattaa
     5221 ttcccgaatc attcgcagat tttcctccga atcggcttcg aatcttaaaa ccagaatcgc
     5281 ctgcgtgttc gacgcgcgca ccaagcccca gccgccggca aacaaaattc tcgcgccgtc
     5341 tatcgtgatg acttcgttgg tttttgaaaa ttcctcggcg atttgccgga cgacgacgaa
     5401 ttttttttct tccgggcaat cgacgcgaat ttccggcgtg gaaacgccgg cgggcaaatc
     5461 ggaaagcatc tgagataagc tcaaatcggt tttcgacaaa atttccagaa ctctcgcgcc
     5521 cgcgtaagtc gcgtcgtcaa aaccgtaaaa gcggtcggcg aaaaagatat gcccgctcat
     5581 ttcgccagcc agaattgcgc cggtctcctt cattttcgac ttgattaagg aatgcccggc
     5641 tttccacatc agcggaatgc cgccgtgttt ttcgatgtcc gtaaacaaat tctgcgagca
     5701 ttttacttcg gcgataatcg tcgcgcccgg attcgccttt aaaaccgagc gcgacagcaa
     5761 aaccatcaat tcgtcgcccc agataattct tccggtttcg tcgatgacgc caatccggtc
     5821 gccgtcgccg tcaaaggcga tgcccaaatc cgcgccgctt cttttcaccg cttcaatcgc
     5881 atcgagcaga ttttcgacga cggtcgggtc gggatgatga ttcggaaaat ttgaatccgg
     5941 ctcggtgaaa agctcgatta gctcgattcc taactcttta taaatcggga cagccgtcac
     6001 gccgcccatc ccgtttccgg catcgacgac cgcttttaat tttttatttc cgagcgagac
     6061 gcgcgccaga atttcgcgcc ggtaatctcc cgaaacttcc agagcctcga cctttcccgc
     6121 gccttccgca aattttccgt taaaagcgat ttccttaatt tgctgaatct gctcgccgaa
     6181 caacgcgtct ttgcccagac aaattttaaa accgttgtgg tcgggcgggt tgtgcgagcc
     6241 agtaatcatc acgccgccgt cgacttcgcg cgtaaaaacc gcgaaataca aaaccggcgt
     6301 cggaactctg ccgatgtcga aaacgtcgca gcccgccgcg tttaaacctc tgacgaataa
     6361 atcgcgaaaa cgcggcgagc tttcccgcgc gtcgaaaccg agcgcgatgc ttttggcgtt
     6421 gttccgacga aaaaaagtgc cgaccgcttt ggctaaaagc gagacggttt catcggtcaa
     6481 atgctcgccg acgatgccgc gaatgtcgta ttccctgaaa atatttggat tcataaagtg
     6541 cgcgtgaatc gttaatcgta aatcgtgaag cgtaaatcgt caattatgaa tcgagaatcg
     6601 gcggaagact tccggttgac gagcggcgat ttaccgttga cgagcggcga ttctcgattg
     6661 acgcgccttt tgttatactc aaaatttgcg tttgggcaac acgggcggcg cttcagggca
     6721 tcgaaacgtg ttgagcttcg taaatattcc gttataaatt atgaaaaatc tggctcgttt
     6781 ttttggattc tcaggtctgc ttttaagttt cgtcttgacg gttccggcgc agacgccgcg
     6841 caccagcccg acgccgccgc gcggcgacga cgacggtcag gtcagaacgt ttgaggttcg
     6901 tctgcccgtc acggtcaaag ataaaaagaa aaatctcgtc agcgggttga agcgcgaaga
     6961 ttttctggtt tttgaagacg gcgttcagca gaaagtgacg tttttcagcg atgaaaaaac
     7021 gaatccgccc gtctcggtcg gcgttctgat ggacacttcg ccgtcaaccg cgggcaaaat
     7081 gaaattctcg aaagaagcgg cgaaaaattt tatttacacc gtgacgcgca tccgcaaaga
     7141 ccaagccgcg tttatgactt tcgaccacga aatcaatctg ctccaggatt ttacagacaa
     7201 gctcgatttg ctcggcaaag ccatcgacgg cgtcaaaaag gtcggctcgc aaaccgcgct
     7261 ttacgacgcc atctggcaat ttacggatga aaagctgcgc aacgctcccg gcaggcacgt
     7321 gattgtcatt atcacggacg gcgacgacac gtttagccgc gccgatatca acgacgccat
     7381 cgacatcgcc cagcgaaccg aaacgacgat tttcgccatc tcaaccaagt ccggatttct
     7441 gggcagcgtt cccggcgtcg aagccggaac ggtcaaggac gaaggcgata aactgctcga
     7501 ccgcctgtgc cgcgaaaccg gcggcgaagc cttctattcg ggcgatatgc tcgcgctcga
     7561 acgagcgctg gacaaagtag caaaagaatt tcgcggtcaa tacatcgtca cctatcgtcc
     7621 ggcaaatcag gaatacgacg gcagaaaacg caaagtcgaa gtgcgtttcg cggataagca
     7681 gaaagcggac gattaccgga ttcgcacaaa gaccgagtat cgggctgtta atgacgggtt
     7741 aaaaaagaat tgatgtagcc ggaggcgaag cgaaaaaagc gaagaaatat tcccgcaatc
     7801 gtaaatccgg aatcaaaagc cgcgttcgta ataagagagg tagaaatgtc aggctgtaaa
     7861 aatttaacgg ttttgtcaat tggattgttg tttgtcgcgt cgactctgtt cggctggaac
     7921 ttttccgccg cgcagcagca gcaaacgacc gcgccgacgc aaaaacaatc cgtgccgtcg
     7981 ccgaccgcga cgcccggcac cgaagtagtc gaagaagaag acgagccgat taaaatcgac
     8041 acggagctgg tcaatgtgct tttcaccgcg caggacaata accggcggct tctgaccgac
     8101 ctcaaaaaag aagacatccg cattctcgaa gacggtcagc cgcagcaaat tttcaccttt
     8161 gcgcgccaga cggatttgcc gctttccatc gctatcgtga ttgatacgag ccaatcgcag
     8221 cagcggacgc tgcccgaaga aaaagatgcc gcgaaatcat ttgtcgaatc ggtcgtcaga
     8281 cccgaaaaag acgaagtcgc ggtgctttcc tttacgggcg agaccacgct ggagcaggat
     8341 ttgaccggaa acgttcagcg cctgcgccgc gccatcgacc gcgtcgagtt cgttcccgcc
     8401 gccggaactt taaacggcgt gctgattgga acgccgccga tttcgggcag aagccagcag
     8461 gtcgccggtt cgaccgcgat ttgggacgcg ctctgggtca cgtccaacga agttttgtcg
     8521 aaagcgccgg aaagaacgcg ccgcgcgatt gttctgatga gcgacggcgt taatactttc
     8581 ggaaataaaa agctggaaga tgccgtcaac caggcgataa aaaacgacgc cattatttac
     8641 agcatcggca tcggtgatta ctactacggc ggcatcgacg aaagcgcgct cagaaaaatc
     8701 tcggagcgca ccggcggacg cgcctttttt cccagagacg aagccgaatt gcgcaaggct
     8761 ttcacgcaga ttcaaatcga aatgcgctcg cagtatttga ttgcttacga gccttcgaat
     8821 caaaaacgcg acggcacttt tcgcaaaatc gagattcagg tctcgaattc cgaattaacc
     8881 aaacagaaaa tccgcctgac gcaccggcag ggatattttg ccaaaaccgc atccattcag
     8941 aataagccgg cgaaatagtt ttccggcgaa aacaaaagag cgaaagcggc tagttcaact
     9001 atccgctttt tttattttcg atacagggaa aatctccgga gaaacccgac cgattctaac
     9061 ggctttgcct ataaactcga tttgcctttt aaaggctgga ctattatcag gaaaaatatt
     9121 gaccccaatc taatcgttta aataaaattc gtaaccactt ttaacccgtc gataacttca
     9181 acgttacagc taagccaatg tccgacgtct aaatattcca atctttgtcg agatacagga
     9241 gttttcagtc cataatatct atagaaatct tcctgcataa tatagaaatc gtcttctgct
     9301 ccattagata atttatacat ccacttaaag ttacccgtat aatttatatc tactatttta
     9361 agtctcatac aagaacacga cctttatcta cagtttcatt agcttaattt tctctgtatt
     9421 ctttgtggct aagtttttac tgcggcagcc cgattttttt caataaatcc gtaaagcgcg
     9481 aatcgtttcg gaggctttta aatgccgggt cggttgccag atactgcaat tgcaaatcgt
     9541 gcagggcaaa cgctttttcg agcgaggcga aagctttttc gcgcatctct aaagccgtgt
     9601 aaagaaccgc cagctcggcg ggcgaaacgt aactttctga agtttccagc tcgctgagaa
     9661 tcgcctgcgc tttctcgcgc tcgcccgcgc cggcataagc cgcgccgaga taaattttat
     9721 tgctcggcgt ttcctgaccc aaactgaagg ctttccggta agcctcgata gcttcggcaa
     9781 acattttctt tgccgagtag ttatagccga gataaagatg cgcgaaagaa aaattagggt
     9841 cgaattccag tgtttcccga agaattttta tcgcctcgtc gtatctgccg gcaaagtata
     9901 aaaccaagcc gtaatttccg ttaatgattg tggaaagcgg gtcgagctct ttggcgcggc
     9961 ggatttcccg gacggcttcg tcgtggcgtc ccgtgcggct cagatagccg gaataaccga
    10021 tgtaggcgcg ggcgagattc ggatttaatt cgatggcgcg cttgaattca aactcagccg
    10081 cagaccattc ccagccgttc attttcagaa acgcgagcgc gaaatgcgcg tccgccagag
    10141 tttcgtcgag ctccagagat ttcagcgccg ccgcttccgc tttcggcgta aattctttcg
    10201 ggtcgagcga gccgctcccg accagaattc gataagtaaa agcaagctcg gcgtaggcga
    10261 gcgcgtaatt cgggtcgatg gcgatcgcct cgttaaaata ttcgacggct cgtttgcggc
    10321 tttccgttcc gcctttggcg cgataaaacc gtcctttcaa taaaagctcg taggcgacgg
    10381 aatttttcgt ttcgtggcgg acgagttgtt tttgctcgct caaggtcagg cggggatgga
    10441 gcttgtcggc gatctcgcgc gaaatatcgc tctgaatcat aagcaaatcg tcggcgcggc
    10501 ggctgtattg ctcgccccag atttgcgtat tgtcgcgcgc gtttatcaat tcgacgctga
    10561 tttgcagatt ttcgccgcgc cgcatcaccg agccggttaa aatcgtcgcg acgtttaaag
    10621 ttttggcgat ttcgcgcaga ttttcggcgt tgtatttgta tttaaacgac gagctgcgcg
    10681 cgattacctt cacgtccgaa agctgcgaaa gattgttgat aagcgattcg ctgatgcctt
    10741 cggaaagata ttccatctcg gcgtcgccgc cgctgttgcc gagcggcaga acggcaatcg
    10801 agcgataatc cgcgcccgcg ccgagccgaa aatacgtcga atacagcaaa aatccgcccg
    10861 caatcagagc gattatcaaa accgccgaaa ccggattaaa attgccgtcc gtttttccga
    10921 actgccagac gagcagggca atcaaaccag ccgccggaat gattattaaa gcgggcggca
    10981 cgtatttttt tacgggcgcg ggaatttccg gcgcgtccgg cgcgtcgatt ctggtcgcaa
    11041 actgccgcgg gcgcaaagac gcctgagttc gataaccgtt ttgcagatta ttctgcgtga
    11101 cgatgccgga ttttccgagc cgctttgctt caaattcgag ttcctgacgg aaatctttaa
    11161 tttcgccgag catctcgggc attttttgat aacgctcggc aggatttttt cgcagcgctt
    11221 tgataatgat gctgctcaat tcgctcggcg tttccgaaac gtaatgcgtc accggcacga
    11281 gattgctttt caaaatagag gcgattacgt cgctcgccgt ttcgccttca aacggcaatc
    11341 gggcggttat catctcgtat aaaataacgc ccaaactcca gacatcgctt cgctcgtcaa
    11401 tctgttttcc gcgcgcctgt tcgggcgaca tatagaaaac cgttcccatg accgttccgg
    11461 gctgcgtgtg aacgtaaggc tgcgtgacgg cgtcggggtc gtgttcttcc gatttttcga
    11521 taaattttgc cagaccgaaa tcgagaactt tgaccagaga gtccgcgcgc agcataatat
    11581 tttccggttt gacgtcgcgg tgaataacgc ccgccgaatg cgccgccgcc agcgccgaag
    11641 ccgcctgctc ggcgatgttc agcatttcgg tcaggctcaa tctttcgcgt tttaattttt
    11701 cgcgcagggt ttcgccctcg acaaattcgg tggtgataaa atgcagcccg tcttctttcc
    11761 cgaattcgta gacggtcaga atattcggat ggttcagcgc cgaagccgtt tccgcttcgc
    11821 gctcgaagcg ccgcagcagg tcgtagtcgt cggcaaactt tgccgacaga attttcagcg
    11881 ccactttgcg gttaaggctt ttgtcctgag ccagatacac ttcgcccata ccgccctttc
    11941 cgattaaaga aagaaccgcg tagcgcccaa aatttttacc gctttcgagc ataaaaagat
    12001 agcgcattat agcattgtcg ctgccgttta aacagaaacg cgagtcttga aacggttgaa
    12061 gtgaaaggaa gattagcggc gctaaacggc gggaaaaaag aattaagaaa tccagccgtc
    12121 gccgccgcgc ggaaatttct tgcctgcaaa ctttggaacg tggtactttt acgaagtatt
    12181 ctctgtttcc cagcggcttt ttatccggta aaggagcgta tttcgttatg gcgcgtatca
    12241 atttctgcag ccgagccgtt ctggttccgg ctttgttcgc cttgtccttt tttatttcgg
    12301 gctgcgcgag ttccgcgggc agtcggtatt tcggaaaaac gcaggctccg aaagataatg
    12361 ttctgcgtta tatctccggc tcggagccgg aatcgctcga cccgcacgtc tccagcggtc
    12421 agccggaagc gcgtgtttat ttggcgattt acgacggctt ggtcgaatat catccgaagg
    12481 atatgcaccc gattccggca attgccaaaa gctgggaaat cagcccgaat gtcgatgaat
    12541 ttatttttcg cctgcgcgct gacgcgaaat ggagcgacgg gacgccgatt acggcgcgcg
    12601 atttcgttta cagcttccgg cgcggatttg cgcccgaaac cgcttcgcgc acggcgagcc
    12661 tcggctat
//
LOCUS       DASWNE010000450         3489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_298242, whole genome shotgun sequence.
ACCESSION   DASWNE010000450 DASWNE010000000
VERSION     DASWNE010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298242"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1489)
                     /locus_tag="VGB68_09025"
     CDS             complement(<1..1489)
                     /locus_tag="VGB68_09025"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0049413.1"
                     /translation="MSFVSVVAFLVSFVSIPETQAKVVVQNQSGLGVIKGVVRDESGK
                     PIADAMVAVFRVGTSKLLKQVRSAADGSFLTKILPGTYTVLAVAQGFNPVTLAEVQVN
                     RSAELTYGFKLERAGSGNTLPEKRPDRTSPKWVIRSAQIRRSIYQNREDETKSDEIKI
                     DETTTAENKNVEESTGIVEEDETETRRKGQSVVETFVADSGAGGVYTGFNFATLQPLG
                     ENTEIIIVGQTGTGKTAPQRFETTVKFRPNESHQLRLTSSAARLGRIEETNEQLGQIS
                     FQALDEWKVREGVILVFGVDYSRFFGASDDFSVSPRFGLQFDIDAKTRFRTAFTTQTE
                     ERTWSHAIELEDSAVVFREQIAPPVIAVEDGRAVMNKSRRLEFGVERILDNKSSIEAT
                     AFFDSVSSRGVGLVNLPFNLLSPEDTFVANQQGKTQGVRFVYSRRLNGILSASAGYSF
                     GKGQKLSPQAVTNPADVFENDFFQTFVGQLNANLSTGTKVKTIFRL"
     gene            1869..1959
                     /locus_tag="VGB68_09030"
     tRNA            1869..1959
                     /locus_tag="VGB68_09030"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1903..1905,aa:Ser,seq:gct)
     gene            2115..2738
                     /locus_tag="VGB68_09035"
     CDS             2115..2738
                     /locus_tag="VGB68_09035"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HEY0049414.1"
                     /translation="MYIPKFFAETDEENLLAFMREFNFATLISSENDFPTATHLPFII
                     EKRGGKIILSAHLAKANLQWKQFENKDVLVIFHEPHAYISPLLYGEKNNVPTWNYVAV
                     HAYGKVETFKTAEENLLFLAKMVEAFDPGYFQTDWQEISADYKINLARGVVAFEIEVA
                     DLQGKKKLNQNKPGKSAENVIKAFENSDRENEKLIAGYMKEVHQNTD"
     gene            complement(2963..>3489)
                     /locus_tag="VGB68_09040"
     CDS             complement(2963..>3489)
                     /locus_tag="VGB68_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049415.1"
                     /translation="ILTYATRFDGSLFTAPLMTLANLYLLAWNYEKWKYILPFNRQAN
                     DDEAAKQNSLEIDVQTQAESANRRAGFFRNAISSLYARLRTFLKQLFGDRFPTLFFAG
                     VFAAVVVCVAGIPSLYRVKPRNTFAQCVKQFKNTSRTGAGENFCHCIHTSGEPLGKCL
                     NEYNNAPDDAIGTP"
BASE COUNT          879 a          866 c          882 g          862 t
ORIGIN      
        1 acaggcggaa aatcgttttt acttttgtgc cggtgctcaa attcgcgttg agctgcccga
       61 caaaggtctg gaaaaaatcg ttttcgaaaa cgtccgccgg attcgtcacg gcttgcggcg
      121 agagcttttg ccctttgccg aacgagtaac cggctgaggc gctcaggatt ccgttcagac
      181 ggcgcgagta aacaaagcgc acgccctgcg ttttgccctg ctgattggcg acaaaagtgt
      241 cttcgggcga caataagtta aacggcagat tcaccagccc gacgccgcgg ctgctgacgg
      301 aatcaaaaaa cgccgtcgct tcgatgctgg atttattgtc cagaattctt tccacgccga
      361 attcaagccg tcggctttta ttcatcaccg ctctaccgtc ttcgacggcg atcaccggcg
      421 gtgcaatctg ctcgcggaaa acaaccgcgc tgtcctccag ttcgatggcg tgcgaccacg
      481 tgcgttcttc ggtttgcgtg gtgaaagccg tgcggaaacg agttttggcg tcgatatcaa
      541 actgcaagcc gaaacgcggg ctgactgaaa aatcgtcgct cgcgccgaag aaacgcgaat
      601 aatcgacgcc gaaaaccaga atgacgcctt cgcgcacttt ccattcgtcg agcgcctgaa
      661 aagaaatttg tccgagctgt tcgttggtct cttctatcct gccgagccgt gcggcggaac
      721 ttgtcaggcg cagctgatgc gattcgttcg gtcggaattt tacggtcgtc tcgaatctct
      781 gcggcgcggt tttgcccgtt ccggtctgac cgacaataat aatttccgta ttttcgccta
      841 aaggctgaag cgtggcgaaa ttaaatccgg tgtagacgcc gcccgcgccg gaatcggcaa
      901 caaaagtttc gaccaccgat tgccccttgc ggcgcgtctc ggtttcgtct tcttcgacta
      961 tgccggtgct ttcctcgacg tttttgtttt cggcagttgt cgtttcgtcg attttgattt
     1021 cgtcagattt ggtttcatct tcccgatttt gataaatcga gcggcgaatc tgcgccgagc
     1081 ggataaccca tttcgggctg gtgcggtccg ggcgtttttc cggcagagtg ttgccgctgc
     1141 ccgcgcgctc cagtttaaag ccgtaagtca attcggcgga acgattcacc tgcacttccg
     1201 ctaaagtgac cggattaaaa ccttgcgcga ccgctaaaac cgtataagtt ccgggaagga
     1261 ttttcgtcag aaagctgcca tccgccgccg agcgaacctg ttttaaaagc ttcgacgtgc
     1321 cgacgcgaaa aacggcgacc atcgcatcgg caatcggctt gcccgattcg tcgcgcacga
     1381 cgcccttgat gacgcccaag ccggattgat tttggacgac tacttttgcc tgcgtttcgg
     1441 gaatcgaaac aaaagacaca agaaaagcga cgacagagac gaaactcaat cgtataatcg
     1501 ttttactttg cctgattaaa gaaaacatta aattaaagcc atctcctttt cgccgagttc
     1561 ggggagactc ttgcataccg gagcgctaaa aacttttgcg ttagtcgaaa aaacagactc
     1621 tacgcgtacg ttctgctatt ttcgtgatta aagtcaaatg tgtcaagccc ttcgacaccg
     1681 taaactctta ttttgcattt cgcgtaaact ttcatttgtt tgatgcgccg gcttcggcga
     1741 aggtttgtcg gcgacggcgc aaaaatagtt ttcgtcaaaa taaacacccg aataggacgg
     1801 cgaacttgac aaagtttgct caaagattag aattaaaaat tgcttttctt gaaaagcttt
     1861 cctgcaaggg agacgtggct gagcggctga aagcggcggt ttgctaaatc gttaagggtc
     1921 aaaagccctt cacaggttcg aatcctgtcg tctccgccag tttttaagta gccggtaatc
     1981 ggattagtca gcggttcggt agatttttct cctgagcgct cgcttctgat ttccggctat
     2041 aattatttta atgcgtattt ccaaatataa tcgacggatg acgagaagcc ggttgccggc
     2101 agccgaccgc tgatatgtat attccgaaat tttttgccga aaccgacgag gaaaatttgc
     2161 tggcgtttat gcgcgagttt aatttcgcca cgctgatttc gtcggaaaac gattttccga
     2221 ccgcgacgca tctgcctttt attatcgaga aacgcggcgg gaaaataatt ttatcagcgc
     2281 atctggcgaa agcgaatttg cagtggaaac aatttgaaaa caaagacgtt ttggtgattt
     2341 tccacgagcc gcacgcctac atttcgcctt tgctctacgg agaaaaaaac aacgtcccga
     2401 cctggaacta cgtcgcggtt cacgcttacg gaaaagtcga aacttttaaa acggctgaag
     2461 aaaatctgct ttttctggca aaaatggtcg aggctttcga cccgggttat tttcagaccg
     2521 actggcagga aatttccgcc gactacaaaa taaatctggc tcgcggcgtg gtcgcttttg
     2581 aaatcgaggt cgccgatttg cagggcaaga aaaagctcaa tcaaaacaaa cccggaaagt
     2641 cggcggaaaa tgtcatcaaa gcgtttgaaa acagcgaccg cgagaacgaa aaattaatcg
     2701 cgggttatat gaaagaggtt catcaaaata ccgattgact tgaatatcga accggcagga
     2761 aaaccggaaa gcccggcaat tagaacatta ccaataaacc ggagccgaat aatccccgaa
     2821 tcaaaagaca agcccgacgg tttctaacga gtgagacggc aaatcgaaat tttcgacgaa
     2881 aaataaaaat aaactccccg caatcttttg tttattaaga ccacgaaaga gcttgctcct
     2941 gggtcggaag gcggcaaagg ttctacggtg ttccgatagc atcatccgga gcattgttgt
     3001 attcgttcag acatttgccg agcggttcgc cggatgtatg aatgcaatga cagaaatttt
     3061 cgcccgctcc ggtgcgactt gtgttcttaa attgctttac gcattgcgca aaggtgttgc
     3121 gcggcttgac gcggtacagg ctcgggatgc ccgcgacgca gacgaccacg gcggcaaaca
     3181 cgcccgcgaa gaaaagcgtc gggaatctat cgccgaaaag ctgtttgaga aaagtgcgga
     3241 gccgcgcata aagcgagctt attgcgttcc ggaaaaagcc tgcgcgccgg ttcgcgcttt
     3301 ccgcctgagt ttgaacatcg atttcgaggc tattctgttt tgctgcctcg tcgtcatttg
     3361 cctgacgatt aaacgggaga atgtatttcc atttctcata attccaggcg agcagataca
     3421 aattcgccag cgtcatcaga ggcgcggtaa aaagcgagcc gtcaaaacgt gtcgcgtagg
     3481 tcagaatgg
//
LOCUS       DASWNE010000451         2721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_546139, whole genome shotgun sequence.
ACCESSION   DASWNE010000451 DASWNE010000000
VERSION     DASWNE010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546139"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..507)
                     /locus_tag="VGB68_09045"
     CDS             complement(43..507)
                     /locus_tag="VGB68_09045"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY0049416.1"
                     /translation="MEKKTKIDAEDGKQDLMITREFDLPLELLFKAYVEPEIVEQWMG
                     TKVLKLENKKHGSYQFETTDPKGNKYRFSGVIHEFSANRKITRTFEMENTAFPVQLEF
                     LEFEKLNDDTSKLSVHMVFRSAADRDEMLRLPFAQGLNMAHNRLQDIAGKLK"
     gene            complement(533..856)
                     /locus_tag="VGB68_09050"
     CDS             complement(533..856)
                     /locus_tag="VGB68_09050"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY0049417.1"
                     /translation="MNLRRDVFQAIADPTRRAILLLVASQSMTAGAIASNFDTARPTV
                     SKHLQILTECELLEQEQNGREIYYHLNPEKIKEIADFIEPFREMWDDRFNKLEAVMKN
                     YKSKK"
     gene            1202..1816
                     /locus_tag="VGB68_09055"
     CDS             1202..1816
                     /locus_tag="VGB68_09055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0049418.1"
                     /translation="MGISMLYDSSLEIENLMDSPDDLLSRAQRGDDEAFGLIFLQHHR
                     FIYKFIYAMVGDESAAEELAQETFLCAYKAIHSIRGESKLRTWLCGIAKNTVYKFLRS
                     HRKQGMKSEDEVESLSVIDEKNPLPDKQVLNKELNCRIQSALEKLDDDKRLIFTLKEL
                     QHLSYSEISEITGYTIPKLKTDLHRAKAVMRSALRPYLEVRNEL"
     gene            1806..>2721
                     /locus_tag="VGB68_09060"
     CDS             1806..>2721
                     /locus_tag="VGB68_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049419.1"
                     /translation="MNCEKYLNLIDDLIEGELNEQNAGQVNSHFLVCPQCASYYETLE
                     QEKEIYARYLFEVEPPTDLWIKFQAKVKTGAEPVSQIHETTAAAATVRKTNIFEFFRL
                     YPALGFAVAFIALGIGLWKFMPGGNVGGNEHLAKKESNNIQAISGQSGESSKGESKDL
                     PAKDEIDKPLASAKAFKPVKEGGSLKRKLNDGTANKLVAAGTPKTDRKIISVGVGNYS
                     PVKPSPGKEQLRQLQLSALEKATTQQIEKIELLLRSFRNARVDEGGDFYDVGYEKQQA
                     RKLLERNVRLRQMAENYGTLYTEEILDRV"
BASE COUNT          728 a          587 c          648 g          758 t
ORIGIN      
        1 agaaatccaa tagatgattt tatttttctt tgtcataatg cgttatttta atttgccggc
       61 gatgtcctgc aatcggttat gcgccatatt cagtccctgc gcaaagggca gccgcagcat
      121 ttcgtctctg tctgcggctg atctaaatac catatgcacg ctgagtttgc tggtgtcgtc
      181 gttaagtttt tcaaattcca gaaactcaag ctggacggga aatgccgtat tctccatttc
      241 aaatgtccgt gtgattttcc ggttcgcgct aaactcgtgg attaccccgc tgaacctgta
      301 tttgtttccc ttcgggtcgg ttgtttcaaa ttgataactg ccgtgctttt tattttcgag
      361 tttcagcact ttcgttccca tccattgctc gacaatttcg ggctcgacat acgctttaaa
      421 aagcagttcc agcggcaaat caaattccct tgtaatcatt aagtcctgtt tgccgtcttc
      481 ggcgtcgatt tttgtttttt tttccatcgc attctatttt tttgatttca ttctattttt
      541 ttgatttgta gtttttcatt acggcttcca gtttattgaa cctgtcatcc cacatttcgc
      601 ggaacggttc gataaagtcg gctatttctt ttatcttttc gggatttaag tgatagtaaa
      661 tttctctgcc gttttgctcc tgctcaagca attcgcactc ggtgagaatt tgcaggtgtt
      721 ttgaaacggt cggtctcgcc gtgtcaaagt ttgaggctat cgcgcccgcc gtcatagatt
      781 gcgaggctac caacagaagg atagctctcc ttgtcgggtc ggcgatggct tgaaaaacgt
      841 ctcgtcttaa attcatatgc gtagttactt aactacaaat atacatgaag tcatttaact
      901 acgcaagccc ttccgcgatt ttttttccga caaatgtttt tgcgtcgcgc cggcaggatt
      961 atttgaaaaa taaacgctca gcccgaggcg gtaaagatta taattagacg atatttttgc
     1021 cgtgctttga catatttttt tctcggaaaa aagcgtccgc gtaagaatca agggaaaata
     1081 ataaaacggc ttaaagtttt tcgcatcaat caagggtttt ataatttttt cggaaaattt
     1141 gaaacctttt gacgattttc ggaactaatg attggtaggt ttatttgaga gttgaaagcg
     1201 tatggggatt tcaatgctct atgattcgag cttggaaata gaaaatttaa tggacagtcc
     1261 tgacgatttg ctgtcgcgcg cgcagcgcgg cgacgacgaa gcattcggtc tgattttcct
     1321 tcagcatcat cgttttattt acaaatttat atacgcgatg gtcggcgacg aaagcgcggc
     1381 ggaagaactg gcgcaggaaa cttttttatg cgcttataaa gcaattcatt cgatacgcgg
     1441 cgaatcgaaa ctgcgcacgt ggctgtgcgg cattgctaaa aacacggttt ataaatttct
     1501 ccgctcgcat cgcaaacagg gaatgaaaag cgaggatgaa gtcgaatcgt taagcgtaat
     1561 tgatgaaaaa aatccgttgc cggacaagca ggttttaaat aaggaattga attgccggat
     1621 tcagtcggcg ctggaaaaac tcgacgacga caagcgcttg atttttacat taaaagagct
     1681 tcagcatctg agttacagcg agatttcgga gattaccggc tacacgattc caaaactcaa
     1741 aaccgattta catcgagcca aagccgtgat gcgaagcgcc ttgcgcccgt atttggaggt
     1801 cagaaatgaa ctgtgaaaaa tacctgaatc tgatagacga tttaatcgag ggcgagctta
     1861 acgagcaaaa cgccggacag gtaaattcgc attttctcgt ctgcccgcaa tgcgcgtctt
     1921 attacgaaac gctggagcag gaaaaggaaa tatacgcccg ttatttgttt gaggtcgagc
     1981 cgccgaccga cttgtggatt aaatttcagg cgaaagtaaa aaccggggcg gagccggttt
     2041 cgcaaattca cgagacgacg gcggctgccg cgacggtcag gaaaacgaat atattcgaat
     2101 ttttccgttt atatccggcg ctgggatttg cggtcgcgtt catcgccttg ggaatcggtt
     2161 tatggaagtt tatgccgggc gggaatgtcg gcggcaacga acatctggct aaaaaagagt
     2221 cgaataacat tcaggcgatt tccgggcaat ccggcgaaag cagtaaaggc gaatcgaaag
     2281 atttaccggc aaaagacgag attgataaac cgctcgcttc cgcgaaagcc tttaagcccg
     2341 ttaaggaagg cggttctcta aaacggaaat tgaatgacgg gacggcaaac aaactcgtcg
     2401 ccgccggaac accgaaaaca gaccggaaga taatttccgt cggcgttgga aattattcgc
     2461 ccgtcaagcc gtcgcccggt aaagaacaat tacgacaatt acagctcagc gctctggaaa
     2521 aagcgaccac gcagcaaatt gaaaaaatcg aattgctgct gcgctcgttc agaaacgctc
     2581 gcgtcgatga aggcggcgac ttttacgatg tcggttacga aaaacagcag gctcgcaaac
     2641 tgctggaaag aaacgtccgg ctgcggcaga tggctgaaaa ctacgggact ttatacaccg
     2701 aggaaattct cgatagggtc g
//
LOCUS       DASWNE010000452         5069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_15637, whole genome shotgun sequence.
ACCESSION   DASWNE010000452 DASWNE010000000
VERSION     DASWNE010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5069
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15637"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..1283)
                     /locus_tag="VGB68_09065"
     CDS             complement(21..1283)
                     /locus_tag="VGB68_09065"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY0049420.1"
                     /translation="MSENPYKFVHTFAALADIGQAVAHKRNFQEMIRTSLHLLLGSLA
                     IMRGGVARYSRYGRELNFLAVRNLGDDFPLSVPLEFEDEREFLRAGMSEIEISQAKFL
                     SFFQENQDILDRKRIELAIPLIVRDELVGIVLLGEKATGEAFTEVDREVVCAMARHIG
                     VGIQQRNLMAELERKAGENRQLYDDLRLTYKDTVKAFAAAIDCKDKYTEGHSERVGRY
                     SEIIAQELDWDEKQIEGVAVAGYLHDVGKLAVDRTIINAPYRINAKESAELSKHPAVG
                     YEILLPIHHPFADVPLAAKYHHERLDGRGYPDGLYDREIPYIAKIVNLADSFDAMTTD
                     RPYKRRRTSEEVIEDLWRNSGKQFAPELVTAFCRAMLKELTGERKEKRFRKMLGKDYL
                     DSEKMIPILKDALNGLNGTSSLTLVSLD"
     gene            complement(1429..1752)
                     /locus_tag="VGB68_09070"
     CDS             complement(1429..1752)
                     /locus_tag="VGB68_09070"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0049421.1"
                     /translation="MREFEATVPVPVQTEGETAIVYASDYLNKLSGEKIERECRHQLD
                     AGCRKLIVNFKDTEIVNSIGVSILLGVIDAASVKGASVVFSDVNEDTIQLFEVLGLTR
                     HVSIN"
     gene            complement(1957..4185)
                     /locus_tag="VGB68_09075"
     CDS             complement(1957..4185)
                     /locus_tag="VGB68_09075"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0049422.1"
                     /translation="MLDETQNIKVLSRIAAEDFIGRNAESEELRRTANDETAAQGILL
                     LSAPAAGASELLRQTYDRLFYESKGVIPFYFAFSDKDKTAKQSAVRFLQTFLQQTVAF
                     RRRDGRLLSIAPDVCEISSLAAPIDAHWIQQLISNCKIESDLIDEASFIRMALSAPLR
                     AAANGAPVFVMLDDLHKAENAAGDAALIEELREIYSRAGLSFVFAGRRRFVLSAMQGG
                     NAGLANAKTMRLGELSGADAEMLVENLSVKYAVKINEQTRDLIIRQFQSNPAFIGGIF
                     RAARASENDLDSFFQVQKVTTDALFGGDFGNYYDRLFDEIAPNRKTQAQIIGLLCETD
                     ENNERKTPIEVWNERLAQSFEEVYRLIRLLNIHEIARTGGGSVELNGENALLHDYVEA
                     RRRLEILSEPRALVLGDVLSNALKRAPQMMTRFYRRASAIGLKQLLSVFNCQETPASL
                     FDYAVFKEKHKGAEDAEIQRLMAAESEKIALPQIIYTANTVAFYPPIAQFAETERTAV
                     ALGFEAADYREENEIVWIAAQIDSKLEAARDLTEFWCDRLEAVALMCNFTRYRLWLVA
                     PEGFSDEAIEVLRERGAYGSSRRQVELLVAHLKAENVIKERMSAGEYEIIVPMGDDTE
                     MIAAHAVEEIARRHEFKPAAINQIKTALVEACINAAEHSHSPDRRIYQKFKIEADKIV
                     ITVANRGVKMPTRKVQESDSRPEPTTGRRGWGLKLMRRLMDEVNFEQTDDGTRISMVK
                     YK"
     gene            complement(4547..>5069)
                     /locus_tag="VGB68_09080"
     CDS             complement(4547..>5069)
                     /locus_tag="VGB68_09080"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY0049423.1"
                     /translation="AGEFEQLSVTDDLTGLVNIRYLNERLTEEIKRSERHGFPMSFMM
                     IDVDDFKSYNDSFGHPEGDRALKLVGALLKEGLRGADVAARYGGEEFSILLPQTNLKE
                     ASLIAERIRQKVEETEFPNRRVTISIGVSSCTPGCTMQGLMSEADKALYKAKASGRNN
                     VQIYEESKAETGI"
BASE COUNT         1137 a         1407 c         1240 g         1285 t
ORIGIN      
        1 tctaataaat tttattaacc ctaatccaga ctgaccagcg tcagcgaaga cgttccgttc
       61 aacccgttca gcgcgtcttt taaaatcgga atcattttct ccgaatccag gtaatctttg
      121 ccgagcattt tccgaaaacg tttttccttt cgctcgccgg tcaattcctt gagcatcgcg
      181 cggcagaaag ccgtgacgag ctcgggcgcg aattgtttgc cggaattgcg ccataaatcc
      241 tcgatgactt cttccgaggt ccgccgccgt ttatacggtc ggtcggtcgt catcgcgtcg
      301 aaagaatccg ccagattgac aatcttggcg atatacggaa tttcgcggtc gtataaaccg
      361 tccggatagc cgcgcccgtc gaggcgttcg tgatgatatt tcgccgccag cggaacgtcg
      421 gcgaacggat gatgaatcgg cagcagaatc tcgtagccga ccgccggatg tttcgagagt
      481 tccgcggatt ccttggcgtt gatgcgatac ggcgcgttga taatcgtgcg gtcgacggcg
      541 agctttccga cgtcgtgcag ataaccggca accgccacgc cttcgatttg tttttcgtcc
      601 cagtcgagct cctgcgcgat gatttccgaa taacgcccga cgcgctcgga atgtccttcc
      661 gtgtatttgt ccttgcagtc aatcgcggcg gcaaacgcct tgaccgtgtc cttgtaggtc
      721 agacgcaaat cgtcgtaaag ctggcggttt tcgcccgctt tgcgttcgag ctccgccatc
      781 agatttcgct gctggatgcc gacgccgatg tgtcgcgcca tcgcgcagac gacttcgcgg
      841 tcaacctccg taaaggcttc gcccgtcgct ttttcgccga gcagaacgat gccgaccagc
      901 tcgtcgcgga cgattaaagg aattgccaat tcgattcgct tgcggtcgag aatgtcctga
      961 ttttcctgaa aaaaggaaag aaattttgcc tggctgattt ctatctccga cattccggca
     1021 cgaaggaatt cgcgctcgtc ttcgaattcc agcggcacgc tcaagggaaa atcgtcgccc
     1081 aggtttcgca cggctaaaaa attcagctcg cgaccgtagc gcgaataacg cgccacgccg
     1141 ccgcgcataa tcgccagcga gcccaaaagc agatgcagag aggtgcgaat catctcctga
     1201 aaattccgct tgtgtgcgac cgcctgcccg atgtccgcca gcgcggcgaa tgtgtgaacg
     1261 aatttgtatg gattttctga catttaagat agtttacaga aagttgcgga tagtttacag
     1321 atagttgcgg atagttgcgg atagttagga gattgacgaa agttgaaacg ccgaaactgt
     1381 ttttaacttt ctgcaactac ctgcaactct gcaactatct gcaacttttt aattaatcga
     1441 aacgtgccgg gtcaatccca aaacttcaaa aagctgaatc gtatcctcgt tgacatccga
     1501 gaaaacgacg ctcgcgcctt tgacggacgc cgcgtcaatc acgccgagca gaatcgaaac
     1561 gccgatgctg ttgacgattt ccgtgtcctt gaaattgacg attaatttac ggcagccggc
     1621 gtcgagctga tgacggcatt cgcgctcgat tttctcgccg ctcagtttgt tcaggtaatc
     1681 gctggcataa acaattgccg tttcgccttc ggtttgcacc ggtaccggca cggttgcttc
     1741 aaattctcgc atagccttga ttgtttttcc tttgttttta aagatttgga agcgctttac
     1801 agaaattctt ctgcaatgaa actaaatttc cgcgttcctg tcaacaaaaa taaagtgaaa
     1861 gagtgaaaaa gtgattgagc gaaaaagcag gtcgaacaaa atttaacaaa gctttttcac
     1921 cttttcacct tttcactttt tcactctttc actttttcac ttgtatttga ccattgaaat
     1981 ccgcgtgccg tcgtccgtct gctcgaaatt cacttcgtcc atcaggcggc gcatcagctt
     2041 taagccccag ccgcgcctgc cggtcgtcgg ctccgggcgc gaatcgcttt cctgaacttt
     2101 ccgcgtcggc atcttcacgc cgcgattggc gacggtaatg acgattttat ccgcttcgat
     2161 tttaaatttc tgataaatcc ggcggtcagg actgtgcgaa tgttcggcgg cattgatgca
     2221 ggcttcgacc agcgcggttt ttatctggtt tatcgccgcc ggtttgaact cgtgacgccg
     2281 ggcgatttcc tcgaccgcgt gcgcggcaat catctccgtg tcgtcgccca tcggcacgat
     2341 aatctcgtat tcgcccgcgc tcattcgctc tttgatgacg ttttccgctt tcagatgcgc
     2401 caccagcaat tcgacctgcc tgcggcttga accgtaagcg ccgcgctcgc gcaaaacctc
     2461 aattgcttcg tcggaaaagc cttccggcgc gacgagccac aagcgatagc gcgtgaaatt
     2521 gcacatcagc gcgaccgctt ccagacggtc gcaccagaat tcggtcaaat cgcgcgcggc
     2581 ttcgagcttc gaatcaatct gcgcggcaat ccagacaatc tcgttttcct cgcggtaatc
     2641 cgccgcttcg aaaccgagcg ccacagccgt ccgctcggtt tcggcaaact gcgcaatcgg
     2701 cggataaaaa gcgaccgtgt tcgccgtgta gataatctgc ggcaaagcga ttttttcgct
     2761 ttccgccgcc atcagccttt gaatttcggc gtcttccgcg cctttgtgct tttccttaaa
     2821 aacggcgtaa tcgaaaaggc tggcgggcgt ctcctggcag ttaaaaaccg agagcagctg
     2881 ttttaaaccg attgccgagg cgcggcgata aaaacgcgtc atcatttgcg gcgcgcgctt
     2941 cagagcgttc gacaaaacgt cgccgagaac aagcgcgcgc ggctcggata aaatttcgag
     3001 ccgtcggcgg gcttcgacat aatcgtgcaa aagcgcgttt tcgccgttca gctcgacgct
     3061 gccgccgccc gttcgcgcga tttcgtgaat gttcagcagg cgaatcaggc gataaacttc
     3121 ttcaaacgat tgcgcgagcc gctcgttcca aacctcaatc ggcgttttgc gctcgttgtt
     3181 ctcgtcggtc tcgcacaaaa gtccgataat ctgcgcctgc gttttccggt tcggcgcgat
     3241 ttcgtcgaaa agacggtcgt aataattgcc gaaatcgccg ccgaaaagcg cgtcggtcgt
     3301 cactttctga acctgaaaaa aactgtccaa atcgttttcg ctcgcccgcg cggcgcgaaa
     3361 aatcccgccg ataaaagccg gattcgactg aaactgccgg atgattaaat cgcgcgtctg
     3421 ctcgttgatt ttaacggcat atttgaccga gagattttcg actaacatct cggcgtccgc
     3481 tccggaaagc tcgccgagcc gcatcgtttt cgcgttggca agaccggcgt ttccgccctg
     3541 catcgcgctc aaaacaaaac gacgtctgcc cgcgaaaaca aacgaaagac cggcgcgcga
     3601 ataaatctcc ctgagttctt cgataagcgc cgcgtcgccc gccgcgtttt ccgctttgtg
     3661 caaatcgtcg agcatcacga aaaccggcgc gccgttggcg gcggcgcgca acggcgcgct
     3721 caaagccatc cggataaacg aggcttcgtc aatcaaatcg ctttcgattt tgcagttgga
     3781 aatcaattgc tgaatccaat gcgcgtcgat cggcgcggca agactggaaa tttcgcaaac
     3841 gtcgggcgca atcgaaagca gccgcccgtc gcggcgccga aaagcgacgg tttgctgaag
     3901 aaaggtttgc agaaaacgaa ccgccgattg tttcgcggtt ttatccttgt ccgaaaaagc
     3961 aaaataaaac gggatgacgc ctttcgattc gtaaaaaagc cggtcgtaag tctggcgcaa
     4021 aagctcgctc gcgcccgccg ccggagcgga aagcaaaaga attccctgcg ccgcggtttc
     4081 gtcgttcgcc gttcgccgga gctcttccga ttcggcgttt ctgccgatga aatcttccgc
     4141 cgcgatgcgc gatagaactt taatattttg agtctcgtca agcatagctt cgtcaaaaac
     4201 acacgcagcg cgaacccgga aactgcgcgg cgataaagag attaggcgca agcgggcgcg
     4261 aaagagcgcg aacggttttt tgtttttttc tgctttgcac aaaaataaag aaaatcgaga
     4321 acaattccga tggctaattg taaattctca attattaatt ctaaattact tcacaaaaga
     4381 taccgaacat caatttagaa tttacaattt acaatttaca attttccctt tctttatttt
     4441 tgtgcaaagc cgtttttttc gtttgtttcg tatatttcac gaaaaatagt tttattttct
     4501 ttttcgtgcc cgttcgtatt ggttcgcggc ttatcttcag tcgttcctat attcccgttt
     4561 ccgcttttga ttcttcgtaa atctgtacgt tattccgacc gcttgccttc gctttataaa
     4621 gcgctttatc ggcttcggac atcaagccct gcatcgtgca gcccggcgtg caggacgaaa
     4681 cgccgatgct gattgtcacc cggcgattcg gaaattccgt ttcttcgact ttttgccgga
     4741 tgcgctcggc gattaagctc gcttccttta aattcgtctg cggcagcaga atcgaaaatt
     4801 cctcgccgcc gtaacgcgcc gccacgtccg cgccgcgtaa gccttctttc aaaagcgcgc
     4861 cgaccaattt caacgcccgg tcgccttcgg gatgtccgaa actgtcgtta tacgatttga
     4921 aatcgtcaac gtctatcatc ataaaactca tcggaaatcc gtgccgctcg gaacgcttga
     4981 tttcttcggt cagacgctcg ttcaaatacc ttatattgac taatccggtc agatcgtccg
     5041 tgaccgaaag ctgctcgaat tcgcccgcc
//
LOCUS       DASWNE010000453         4757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_15696, whole genome shotgun sequence.
ACCESSION   DASWNE010000453 DASWNE010000000
VERSION     DASWNE010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15696"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /gene="hisC"
                     /locus_tag="VGB68_09085"
     CDS             complement(<1..868)
                     /gene="hisC"
                     /locus_tag="VGB68_09085"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEY0049424.1"
                     /translation="MFKLENLVRENIKRLEPYSSARKEFAGEAQIFLDANENSFGSPA
                     LDAGDYNRYPDPLQREIKQKLAISGGAEPTEIFVANGSDEAIDLLLRIFCEPQKDNIL
                     ICPPTYGMYEVSAEINDVKIKRANLTEDFKLDLKAIEKATDASTKLIFVCSPNNPTGN
                     SFTRFEILELLKNFGGIVILDEAYIHFSGEKSFVGEINNFPNLVVLQTFSKAWGLAGL
                     RVGLAFANKEIIKLFNKVKPPYNVSEIAQRAILEALGNGEKIEKTIAEIVSERERLIE
                     NLGGFSFVRKIYP"
     gene            complement(899..2215)
                     /gene="hisD"
                     /locus_tag="VGB68_09090"
     CDS             complement(899..2215)
                     /gene="hisD"
                     /locus_tag="VGB68_09090"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEY0049425.1"
                     /translation="MKTISYPKKNTWNEILARPTLDTKFLERTVANILADVRQHGDEA
                     LKHCARHFDKVDLVDFLVSESEFDEAEKRISTELKEAIRTAKANIEKFHAAQIDESRV
                     IETTPGVFCWRKSLPIEKVGLYIPAGSAPLFSTVLMLAVPARLAGCPEIILCSPPDTS
                     GKINDATLYAARACGATKVFKIGGAQAIGAMAFGTKQVPKVYKIFGPGNQFVTEAKQQ
                     ITKFGVALDMPAGPSEVAVLADESSVPAFVAADLLSQAEHGSDSQVLLVTTSEKVIAE
                     TLVEVEKQLELLPRKTAAAKALENSKAILVESIETAIELLNEYAAEHLILAVENADEI
                     AEKIVNAGSVFLGNYSCESAGDYASGTNHTLPTGGYAKCYSGVSVDSFVKKITFQKLT
                     AEGVGNLGRTIECLAEAEGLAAHKNAVTIRLEQIYDGDNKAYKKLI"
     gene            complement(2269..3132)
                     /gene="hisG"
                     /locus_tag="VGB68_09095"
     CDS             complement(2269..3132)
                     /gene="hisG"
                     /locus_tag="VGB68_09095"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEY0049426.1"
                     /translation="MENQKLKIALQKNGRLSEQSHKLLRRCGISFSNGLGKLRTEATN
                     FRLEIFFLRDDDIPEYVADGVADVGIVGENVLAEKHKAVDAVEKLGFGECRLSLAVPK
                     NFVYESVSDLNGRRVATTYPKILTDFFAEKNIRAEIHTISGSVEIAPSIGLADAVCDL
                     VSSGSTLFTNGLREVETVMHSEAVLIARKNLDSDLRAILEKLLFRIRSVKAAARNKYI
                     LLNAPNEKLGEIAALLPGMKSPTVLPLNEPGWSSIHSVVDESDFWEIVEQLKAAGAEG
                     ILVLSIEQMIR"
     gene            complement(3651..4145)
                     /locus_tag="VGB68_09100"
     CDS             complement(3651..4145)
                     /locus_tag="VGB68_09100"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HEY0049427.1"
                     /translation="MNFEEITNDFIGTWTGESILYLSWLPSPEFHSQSELTAASVVRD
                     KFLTFNYTWEHENTAHEGLLLVGYDSKQEIFNGAWVDSWHSNVKPLILSGKVDEKGDI
                     DLRGSYEVPNHPDWGWRIVVGTSESNLRIKMYNITPEGEEDLAVQSDYKRLLQEAGVN
                     KENR"
BASE COUNT         1151 a         1149 c         1130 g         1327 t
ORIGIN      
        1 acggataaat ttttcgcacg aacgaaaagc cgccgagatt ttctatcaat ctttcgcgtt
       61 cggaaacgat ttcggcaatc gttttttcga ttttttcgcc gtttccgagc gcttccaaaa
      121 tcgcccgctg cgctatttca gagacgttgt agggcggctt tactttatta aaaagcttta
      181 tgatttcttt attcgcgaaa gccagaccga cgcgcaagcc cgccaagccc caggcttttg
      241 aaaaagtctg taaaactacg aggttcggaa aattgttaat ttcgccgaca aaggattttt
      301 ctccggaaaa gtgtatgtag gcttcgtcaa ggatgacgat gccgccgaaa tttttcaaca
      361 attcgagaat ttcgaaacgg gtgaaactgt ttccggtcgg gttattcggc gagcagacga
      421 aaatcaattt tgtgctcgcg tccgtcgcct tttcaatcgc tttcaaatca agtttaaaat
      481 cttcggtcaa atttgcccgc ttgattttta cgtcgttgat ttccgccgaa acttcataca
      541 ttccgtaagt cggcgggcaa atcagaatgt tgtctttctg cggctcgcaa aaaatcctga
      601 gcagcaaatc tatcgcttcg tcgctgccgt tggcgacaaa gatttcggtc ggttcggcgc
      661 cgccggaaat tgccagtttc tgcttgattt cccgctgcag cgggtcggga taacgattgt
      721 agtctccggc gtcgagcgcg ggcgagccga aactgttttc gttggcgtca agaaaaatct
      781 gcgcctcgcc cgcgaattcc tttcgggcgg aggaatacgg ctcaaggcgt tttatgtttt
      841 cgcgaacgag attttccaat ttaaacatat caataaagtc cggacgcaga aaattaaatt
      901 aaatcaactt tttatacgct ttgttgtctc cgtcgtaaat ttgctccaac cgaatcgtga
      961 ccgcgttttt gtgcgctgcc agtccttcag cctctgccag gcattcgatt gtccgtccga
     1021 gatttccgac gccttcggcg gtaagtttct gaaacgtgat ttttttgacg aaactgtcca
     1081 ccgaaacgcc gctgtaacat ttagcgtagc cgcctgtcgg cagcgtgtga ttcgttcccg
     1141 aagcgtagtc gcccgccgat tcgcacgaat agtttccgag aaaaacggaa ccggcattga
     1201 cgattttttc ggcaatttca tccgcgtttt cgacggcgag aatcaaatgc tcggcggcgt
     1261 attcgtttaa aagctcgatt gcagtttcaa tcgattcgac cagaatggct tttgaatttt
     1321 ccagcgcctt ggcggcagca gtttttcgcg gcagaagctc gagttgtttt tcgacttcaa
     1381 ccaatgtttc cgcaatgact ttttcgctgg tcgtaacaag caaaacctgg ctgtccgagc
     1441 cgtgttcggc ttgcgacagc aggtctgcgg cgacaaaagc gggaacgctc gattcgtcag
     1501 ccaaaacggc gacttccgaa ggtccggcgg gcatatcgag cgccacgccg aattttgtta
     1561 tctgttgctt ggcttcggtc acaaactgat tgcccggacc gaaaatttta taaactttcg
     1621 gcacctgctt tgtgccgaaa gccatcgcgc cgatcgcctg cgcgccgccg attttgaaaa
     1681 ctttcgtcgc gccgcaagcg cgcgccgcat acaatgtcgc gtcgttgatt ttgccgcttg
     1741 tatcaggcgg cgagcagaga atgatttcag ggcagccggc gagccgcgcc gggacggcga
     1801 gcatcagcac ggtcgaaaaa agcggcgcgc tgccggcggg aatgtaaagc ccgacttttt
     1861 cgatcggcaa tgatttgcgc cagcagaaaa cgcccggcgt cgtctcgata acccggcttt
     1921 catcaatctg cgcggcgtga aatttttcga tattcgcctt cgccgtccga attgcctctt
     1981 tcagctcggt ggaaattctt ttttccgctt catcgaattc gctttcggag acgagaaaat
     2041 cgacgagatc gactttatcg aaatgccgcg cgcaatgttt tagcgcttca tcgccgtgtt
     2101 ggcggacatc tgccagaatg ttggcgaccg ttcgttccaa aaattttgtg tcgagcgtcg
     2161 ggcgcgcgag aatttcgttc caggtgtttt ttttcgggta actaatcgtt ttcatatttc
     2221 ttattttttc ctgccgcgcc agttcgctaa aacaagctcc gacgtaattt atctaatcat
     2281 ctgttcaatc gataaaacca gaatgccttc ggcgccggcg gctttcagtt gttcgacgat
     2341 ttcccaaaaa tcgctttcgt cgacgactga atgaatcgag ctccagcccg gttcgttgag
     2401 cggcaaaacg gtcgggcttt tcattccggg caaaagcgcg gcgatttcgc cgagtttttc
     2461 gttcggcgcg ttgagcagaa tgtatttgtt ccgtgccgcg gctttgaccg agcgaattct
     2521 aaaaagaagc ttttcgagaa tggctcgcaa atcggaatcg agatttttcc gcgcgatcaa
     2581 gaccgcctcc gaatgcatta ccgtttccac ctcgcgcaag ccgttggtaa aaagcgtgct
     2641 ccccgaactg accaaatcgc aaaccgcgtc cgccagaccg atcgacggcg cgatttcgac
     2701 cgaaccgcta atggtgtgta tttccgccct gatatttttt tcggcgaaaa agtccgtgag
     2761 gattttcggg taagtcgtgg cgacgcgtct gccgttcaaa tcgctgaccg attcgtatac
     2821 gaaatttttc gggacggcga gagacaagcg acactcgccg aaaccgagtt tttcgaccgc
     2881 gtcgaccgct ttgtgttttt ccgccagcac gttttcgccg acgatgccga catcggctac
     2941 gccgtcggcg acgtattcgg gaatatcgtc gtcgcgcagg aaaaagattt cgagccggaa
     3001 attggtcgcc tcggtgcgga gcttgccgag cccgttacta aatgaaattc cgcatctcct
     3061 gagcaatttg tgagactgtt cgctgaggcg accatttttt tgtaatgcaa tttttaattt
     3121 ctgattttcc atcgtaaaaa taaaaaaaac ctggaatttt ctttcaaggt tcgcgtcaag
     3181 ttaaattaat tttcggattt ttgtaaacga agactagctc acgcgatgat gtcgggcgtg
     3241 atgatggtgg cggcaaagtt gaacggcgta aaaattcatt gttctttata ttgaaaacat
     3301 atcgaaaaga tttttgcaac cgccgtgtga ggctaattat aacgcatttg caaaaccgcg
     3361 cctgtaaagc ttagtaatga gcattttcaa acggaagctg aaattcatcg ataaagaaac
     3421 aaacgataaa tttaatcatt attagtccgg ctttcccgaa cgcggaaaaa gcctgaaatt
     3481 gctgttcctc actcgccccg atatctgtga attcccgcag cgacgcaatt aagccggcaa
     3541 gggttagtaa tcaaaaaata aaaaaagccc gtccatatct gaacgagctt taaaataaaa
     3601 taaaaggatg attgcgcccg ccttttattt tcggattgca ggatgagctt ttaacgattc
     3661 tctttgttta cgcccgcctc ctggagcagc cttttgtaat cggattgcac agctaaatct
     3721 tcttcgcctt cgggagtgat attatacatt tttattcgca gattactttc cgacgtgccg
     3781 acgacgatgc gccatcccca atcaggatga ttcggcactt cgtaactgcc ccgcaaatcg
     3841 atatctcctt tttcgtcaac ctttcccgac aagattaacg gtttcacatt tgaatgccag
     3901 gaatcaaccc acgcgccgtt gaagatttcc tgtttcgaat catacccgac gagcagcaaa
     3961 ccttcgtgtg ctgtgttttc gtgttcccag gtgtaattga aggtcagaaa tttatctctg
     4021 accacgcttg cggcggttaa ttcactctgc gagtgaaatt ccggcgacgg cagccaactt
     4081 aaatacaaaa tactttcgcc tgtccaggtg ccgataaaat catttgtaat ttcctcaaaa
     4141 ttcatcaaac gaatcgctcc ttaattttga aaatggtaaa gcgattgcga gattaacacg
     4201 attgagtttt aagcgcaaga ctatgtttca agaatgaaac aagtttttga gcgaaaccgg
     4261 ctgtggatat tttcacccgg ttcgtatgtc cgaaaactct ggttttcgga cactcttttt
     4321 atgagcttca aattgtttta atctataaaa tttagttctg caacttccgg gcagatttcc
     4381 gggcgctctt atttttttag gcgggagcaa agcaaagcgc cgggcacgag cagatattta
     4441 attttagaca tttagatttt ccttgtcagg caggatttga gagtagccga atccaggaca
     4501 gaaaagcgcg ggaatggatt tcgacagttt ttaacgattc tgatttgggc aataaaaaga
     4561 ggctggtttg aagccccgag acaggttatt ttctatgttt aacggttgag ataacgcggg
     4621 cgcgggcgtg tgataattga caagcaggca ctaatcgcca atttttgttt gctgacagcg
     4681 cgcgctttaa tcactaaaag attcttggaa attccgagag tctttttcat tttcacgaaa
     4741 aaccgttgac agcctcg
//
LOCUS       DASWNE010000454         2478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_546346, whole genome shotgun sequence.
ACCESSION   DASWNE010000454 DASWNE010000000
VERSION     DASWNE010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546346"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            249..>2478
                     /gene="lptD"
                     /locus_tag="VGB68_09105"
     CDS             249..>2478
                     /gene="lptD"
                     /locus_tag="VGB68_09105"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEY0049428.1"
                     /translation="MRYKSVFKIPLSILLACVCASFSFSQDPNPVDRKVANPITDTPN
                     INPVAPEQTATPKPTPKIDNANVQEGGDDEVVVYSDRQSAEGEKGKRVVIHQGNVDVR
                     YGVYRLQADKITLYEATDQLLAEGNVVFDKAEDERITGTKAEWNYKTKLGYFIDSTGF
                     TNQTTDGTIIYFTADRVERVSENEIVVKNGKFTACEDAVPRWSFTAREASIKTNDRIK
                     LKQPQFRLRDIPVIPLPFATIPIEKRDRQSGFLTPTVGYSANKGFRLSTAYYQTLGRS
                     ADVTFRAEIYSARGVGFGLDARTRANSRSYFNFGFFAVKDRVFGSRTDENGARKPDQG
                     GSLFYADGIHYFPDGWTAAADVRLTSNLAFRQVFSDSIQQVISPIEVSKAFVNKSWGS
                     YTLNILGRSEEISIPNVFIRTRNLPSISFEKRPSPLSFFDNVYFSFKTTAEGVSRRES
                     VNNLELYRLRTGNDPISTPLLGQRFDIHPQISVPFSTKYFNFTATAGMRVTYYSDSLD
                     NLRRVVSRDEVRKYGEFEFDVRPVALAKNYYGENDAFRFRHTIEPYLTYRLIKGVDNF
                     NRIIRFDYTDTITDTNEIEYGVVNRIFTRRYTEAVTEQAQRLLREKANAENENGKETG
                     GKNPLSVQPYEIFTLTVRQKYFFDETFGEALVPGRRNQISPITQLTFYTFGGTPRRFS
                     PLSIDGTYRPQKTIFVNTRMDVGVQNDGLRAISATVGYDTPLLKVFQTFYYTRAVTLI
                     PSL"
BASE COUNT          609 a          701 c          613 g          555 t
ORIGIN      
        1 aaagacgaat tcgccgttta atattttctg tcgtattcga tgaatcgcgg tcataagttg
       61 agatgaatta cattcgattc atcacaaaaa gccgcaaaaa gcgcaaggct cagaaaatca
      121 ttgaaacaag gaaggtatca agattcttga aattacccgc gccttttgcg gcttttccga
      181 ttctagcaca agactttttt atttgccctg ttccgtgtta gcatcaaatt aattttctgc
      241 gttacaaaat gcgttacaaa tcagttttca aaattcctct ttcgatactc ttggcgtgtg
      301 tctgcgcatc tttttcattt tcccaggacc cgaacccggt cgaccggaaa gtcgccaacc
      361 cgataaccga cacgccgaat atcaacccgg tcgcgccgga acagacggcg acgccgaaac
      421 cgacgccgaa aatcgataac gcaaacgtgc aggaaggcgg cgacgatgaa gtcgtcgttt
      481 attccgaccg gcagagcgcc gaaggcgaaa agggaaagcg cgtcgtcatt catcagggaa
      541 acgtcgatgt gcgttacggc gtttatcgcc ttcaggcgga taaaataacg ctctacgagg
      601 cgaccgacca actgctcgcc gaaggcaacg tcgttttcga caaagccgaa gacgagcgca
      661 ttacgggcac gaaagccgaa tggaattaca aaaccaagct cggttatttt atagattcga
      721 cgggatttac caatcaaacc accgacggca cgattattta ttttacggcg gatagggtcg
      781 agcgggtttc cgaaaacgaa atcgtcgtaa aaaacggcaa atttactgcc tgcgaagacg
      841 ccgttccgcg atggagcttt accgccaggg aagcgagcat caaaaccaac gaccgcatca
      901 agctcaaaca gccgcagttt cgcctgcgcg acattccggt catcccgttg ccctttgcga
      961 cgattccgat tgagaaacgc gaccggcaat cgggcttttt gacgccgacc gtcggttatt
     1021 cggcaaacaa agggtttcgt ctttcgaccg cttattatca aactctcgga cgcagcgccg
     1081 acgtgacttt tcgcgccgaa atctacagcg cgcgcggcgt cggattcggg ctcgacgcgc
     1141 ggacgcgagc caattcgcgc tcgtatttca acttcggttt tttcgccgtc aaagaccgcg
     1201 ttttcggctc gagaacggac gagaacggag cccgcaaacc cgaccagggc ggctcgctct
     1261 tttatgccga cggcattcat tatttcccgg acggctggac ggcggcggct gacgtgcggc
     1321 tgacctcgaa tctcgctttc cggcaggttt tttccgattc gattcaacag gtgatttcgc
     1381 cgattgaggt ttccaaagcc tttgtcaaca aaagctgggg cagctacacg ctgaatattc
     1441 tgggcaggtc ggaggaaatt tcgattccga acgtcttcat ccgaacgcgc aatctgccga
     1501 gcatttcttt tgaaaaacgc ccgtcgcctt tgagcttttt cgacaacgtt tacttttctt
     1561 tcaagacgac cgccgagggc gtttcgcgtc gcgaatcggt caacaatctg gagctttatc
     1621 gcctgcggac gggaaatgac ccgatcagca cgccgctgct cggtcagcgc ttcgatattc
     1681 atccgcagat ttccgtcccg ttcagcacga aatatttcaa tttcacggcg accgccggaa
     1741 tgcgcgtcac atattactcc gactcgctcg acaatctgcg acgcgtggta agccgcgatg
     1801 aagttcgcaa atacggcgag tttgaattcg acgtccgccc cgtcgcgctc gccaaaaatt
     1861 attacggcga aaatgacgcg ttccgctttc gccacacgat cgagccttat ctgacctatc
     1921 gtttaatcaa aggcgtggac aatttcaacc gcatcatccg ctttgattac accgacacga
     1981 taaccgacac gaacgaaatc gaatacggcg tcgtcaaccg cattttcacg cgccgctaca
     2041 cggaagccgt gaccgagcag gcgcagaggc ttctgcgcga aaaagcgaac gccgaaaatg
     2101 aaaacggcaa agaaacgggc ggaaaaaatc cgctttcggt tcagccctat gagattttca
     2161 cgctgaccgt caggcaaaaa tatttcttcg acgagacttt cggcgaggct ctggttccgg
     2221 gcaggcgcaa ccaaatctcg cctatcacgc agctgacgtt ttacaccttc ggcggcacgc
     2281 cgcgccggtt ttcgccgctc agtatcgacg gcacctatcg cccgcaaaaa acaattttcg
     2341 tcaatacccg gatggacgtc ggcgtccaga acgacggact gcgcgccatc tccgccacgg
     2401 tcggctacga cacgccgctt ttaaaagtct tccagacgtt ttactacacg cgcgcggtga
     2461 cgctgatacc gtcgctgc
//
LOCUS       DASWNE010000455         1855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_546394, whole genome shotgun sequence.
ACCESSION   DASWNE010000455 DASWNE010000000
VERSION     DASWNE010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546394"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..452)
                     /locus_tag="VGB68_09110"
     CDS             complement(111..452)
                     /locus_tag="VGB68_09110"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0049429.1"
                     /translation="MGDTSVQKVMSKNSPHGEMGQKYLASGIHVAMRLWENQEPNDDK
                     EERTREYETVGYVISGKAELHLEGQMLLLEKGDSWVVPKGARHSYKILESFTAVEATS
                     PPAQAHGRDEE"
     gene            complement(769..1284)
                     /locus_tag="VGB68_09115"
     CDS             complement(769..1284)
                     /locus_tag="VGB68_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049430.1"
                     /translation="MKLVSIIFLCLFFALATFGQTPEQPQADNEIVGVQEIYLAKDNG
                     EGEAGDAETNFLSTDIPIFCIVQLNSTKPATVKMNFIAVKVAGVKAETKVVSVSYKTN
                     GKQNRVNFTGSPEGAWIAGSYRVDVFVDGKLAGSRELEIQKSATTNPAVKSFQPKTNA
                     KSKPAKRIRKN"
     gene            complement(1377..>1855)
                     /locus_tag="VGB68_09120"
     CDS             complement(1377..>1855)
                     /locus_tag="VGB68_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049431.1"
                     /translation="RRMREYQRTTRECSFNDFQPEIRTAIRNYVEKHELGDIEEDVLM
                     CAETVSEKIKQGFFSKIFGGANYAQNVSMLVTPERLLWCAQDSRNNRVVSAARLSEIE
                     IGDFESSLVEDCGLSVFGFVNRSPERVRAFIGLGAEAAAQKFRRTVKETAAKAKSK"
BASE COUNT          402 a          463 c          457 g          533 t
ORIGIN      
        1 cgccggcaca ggcgcacggg cgcgacgagg aataacttgc gggaaaaccg tttataaaaa
       61 agccttgact gatttagtca aggctttttt ataaacggtt ttcccgcagt ttattcctcg
      121 tcgcgcccgt gcgcctgtgc cggcggcgat gtggcttcga ccgccgtaaa cgattccaga
      181 atcttatagc tatgacgcgc gcctttcggc acgacccagg aatcgccttt ttccagtaga
      241 agcatctgtc cttccagatg cagttcggct ttgccggaaa tcacgtagcc gaccgtttca
      301 tattcgcgcg tgcgctcttc cttatcgtca ttcggctcct gattttccca gagccgcatc
      361 gccacgtgaa tgcccgaagc cagatatttc tgtcccattt cgccgtgcgg cgagtttttc
      421 gacatcactt tttgaaccga tgtatcaccc attgttaaaa cttcctcctt gtttttcgga
      481 tatttcgagc aatagacgtg ccaggcgtcg gcgagcgcga ttgtgatttt ccgtgataca
      541 gatttgtcga tttaatctgc cgggagcgtt tgattatcgt tcagttattt gaaccacgct
      601 tggcagaggg gcgcagttat ttaataaaaa tttggatgcg gattgaatag gattgattaa
      661 gataattcag gattgattaa gataattcag gatttattta aagaaatcct gaataacccg
      721 gtaaaatcgg cgtcccgttc tctgctggta aatctggctc ttctgtgttt agtttttacg
      781 gattcgttta gcgggtttgg atttggcgtt tgttttcggc tgaaaagatt tcaccgccgg
      841 atttgtcgtc gccgattttt gaatttcaag ctcgcggctt ccggcgagct tgccgtcgac
      901 gaaaacatcg acgcggtaac ttccggcaat ccacgcgcct tcgggcgagc ccgtaaaatt
      961 gacgcgattc tgcttgccgt tggttttgta gctgacgctg acgactttcg tttcggcttt
     1021 gacgcccgct actttcaccg cgataaaatt catcttgacg gttgcgggct tggtcgaatt
     1081 aagctgcacg atgcagaaaa tcgggatgtc ggtcgacaga aaattcgtct cggcatcgcc
     1141 ggcttcgccc tcgccgttgt cctttgccag ataaatctcc tggacgccga cgatttcatt
     1201 atccgcctgc ggttgttcgg gcgtttgtcc gaatgtggcg agagcgaaaa aaagacaaag
     1261 aaatattatc gaaacgagct tcacaatcgt ccctccgatg acttgaaaat tgtttgatgc
     1321 ctgccgtatt tattggtgcg gcaagtcaca ttttagttgc ttgaggatgg gaaaaattat
     1381 ttggattttg ctttggcggc ggtttctttt acagtccgcc gaaacttttg tgccgccgct
     1441 tccgcgccta atccgataaa ggctcggacg cgttccggcg aacgattgac gaaaccgaaa
     1501 acgctcaagc cgcagtcttc gaccaggctc gactcaaaat cgccgatttc gatttcagac
     1561 aaacgcgccg ccgagacgac gcgattgttt cggctgtcct gagcgcacca caaaaggcgt
     1621 tcgggcgtga ccagcatcga aacgttttga gcgtaatttg cgccgccgaa aattttactg
     1681 aaaaaaccct gtttgatttt ctccgaaacg gtttcggcgc acatcaagac gtcctcttcg
     1741 atatcgccga gctcgtgttt ttcgacgtaa tttcggattg ccgttcggat ttccggctgg
     1801 aaatcattaa acgaacattc gcgcgtcgtt ctctgatatt ctctcattcg tcttc
//
LOCUS       DASWNE010000456         4964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_685727, whole genome shotgun sequence.
ACCESSION   DASWNE010000456 DASWNE010000000
VERSION     DASWNE010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4964
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685727"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="VGB68_09125"
     CDS             <1..1011
                     /locus_tag="VGB68_09125"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY0049432.1"
                     /translation="RREGEILLAKDVLDKQLIDVDGKRVVRVNDVQLIAVGDEWRVTG
                     ADVSFQGFLRRLMPSGLSLNRPVEVLDWADVGYLATDTATAAVQLKSSKDKLSRLHPV
                     EIAQLAETLSPVHRTEIVESLDNEIAAETLEEMSTENQARILEEMDEERAADILEEMS
                     PDDAVDVLGEMDEEKAQELFNLMEGEEKADVAELMPFESDTAGGLMTTEFVTFPKNLT
                     VSQAIARLREMAETPNMIYYLYVVEEENSWKIAGLITLRSLILADPTYPLSEVMRKEF
                     QSAHPSDSAEDVAQTIAEYNLLALPVIDDLGEIAGIVTVDDAMEILLPKNLDRRLPRL
                     FG"
     gene            1323..1916
                     /locus_tag="VGB68_09130"
     CDS             1323..1916
                     /locus_tag="VGB68_09130"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEY0049433.1"
                     /translation="MKRKSILFVLALFAFVSLANVGSAGDFAVGTVMEDFKLTDINGA
                     EQSFGKLKGEKGTILVFLSAQCPVVKNYNARISAFAKSYKAKGINVVGINSNATESLE
                     WVKSHATENYDFPMLIDKGNVIADKLGANVTPEIYFFDENNKLLYKGAIDNDRSGGNV
                     TNHYLQDAVDAKMAGKAIAKTSANAFGCSIKRAAAKN"
     gene            1923..2855
                     /locus_tag="VGB68_09135"
     CDS             1923..2855
                     /locus_tag="VGB68_09135"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY0049434.1"
                     /translation="MKNSVWAKAKIVILFSGLIFVFAQIFSVELQAQKRRKTSPARTA
                     KKKTPAKRAALRLNLPKVTQIDTTALQSLLKRDGANAKPLLVNFWATWCDPCREEFPD
                     LVQIDADYKGKIDFITISLDYLSEIDGDVPKFLADMKAAMPAYLLKTENESEAIAMVS
                     KDWQGGLPFTILFDASGKEIYVRQGKIKVEAVRAEIDKALNPPQPESAFEKGKLDAEK
                     DLAAGKIIIKTNFISRRRRDAGAEYGITIEPVLSYKAQEIEYLKGYNSKSEAEISKRF
                     GEEVLKKIKPQVPPPNYPADVVELKLVLPGQKIN"
     gene            2960..3457
                     /locus_tag="VGB68_09140"
     CDS             2960..3457
                     /locus_tag="VGB68_09140"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY0049435.1"
                     /translation="MFRKIEDFRKAWEYETESTIKLLNHLTDESLNQKVTENGRSLGF
                     LAWHLVTTLAEMPGHVGLKIDAPLEGETPQTIAEIASAFEKGAKSVSEEVGKNWTDET
                     LLQEDNLYGETWTRGETLGYLLVHQTHHRGQITVLMRQAGLPVSGIYGPAKEEWQAMG
                     MPAMA"
     gene            3592..>4964
                     /gene="purL"
                     /locus_tag="VGB68_09145"
     CDS             3592..>4964
                     /gene="purL"
                     /locus_tag="VGB68_09145"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF002290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEY0049436.1"
                     /translation="MTETKITPEIVAQHNLTAEEYEKILKIMEREPSMTELGIFSVMW
                     SEHCSYKSSRVHLKRLPVTGTRVIVPPGENAGVVDIGDDWCVAFKVESHNHPSFIEPF
                     QGAATGVGGILRDVFTMGARPIAAMNSLRFGPLNEEKNGRRNKTILKGCVDGIAHYGN
                     CFGVPTVGGEVVFDEAYSLNPLVNAFALGIVRKDQIFFGKASGVGNPVLYVGAKTGRD
                     GIHGATMASAEFDDEALEKRPTVQVGDPFLEKLLLEACLEAMRSGAIEGIQDMGAAGL
                     TSSSVEMAARAGTGLELDLTLVPQRETGMTAYEMMLSESQERMLIVAHKGREKQIVEI
                     FNKWDLDAVVVGRVVEGNRLKVFHNGNLEADLPVDAVTDEAPKYNRPMLARIQNSKFK
                     IQNYEARQPKTKDHTEALKMLLAAPNICSKHWVYEQYDHMVRTNTAILPGADAAVVRV
                     KETRRAIA"
BASE COUNT         1322 a         1188 c         1363 g         1091 t
ORIGIN      
        1 cggcgcgaag gcgagattct gctggcgaaa gacgttctgg ataaacagtt gattgacgtc
       61 gacggcaagc gcgtcgtccg cgtcaatgac gttcagctta tcgcggtcgg cgacgaatgg
      121 cgcgtgaccg gcgctgacgt cagctttcag gggtttttgc gccgcctgat gccttcgggg
      181 ctttcgctga atcgtccggt cgaagtgctg gattgggctg acgtcggtta tctggcaacc
      241 gacacggcga ccgccgctgt gcagttaaaa tcttcaaaag acaagctttc gcggcttcat
      301 ccggtggaaa tcgcgcagct tgccgaaacg ctctcgcccg ttcaccgcac ggaaatcgtc
      361 gaatcgctcg ataacgaaat cgccgccgaa actctggaag aaatgtccac cgaaaatcag
      421 gcgcgcattt tagaggaaat ggacgaggaa cgcgccgccg acattctgga agagatgtcg
      481 ccggacgacg cggtggatgt tctcggcgag atggatgaag aaaaggctca ggagcttttc
      541 aatttgatgg aaggcgagga aaaagccgac gtcgccgagc tgatgccttt tgaaagcgac
      601 acggcgggcg gcttgatgac gacggaattc gtcacgtttc cgaaaaatct gacggtcagc
      661 caggcgattg cgcggctgcg cgaaatggct gagacgccga atatgattta ttacctttac
      721 gtcgtcgaag aagaaaattc gtggaaaatt gccggtttga ttacgcttcg cagcttgatt
      781 ttagccgacc cgacttatcc gctttcggaa gttatgcgaa aagagtttca gtcggcgcac
      841 ccgagcgatt ccgccgagga cgtggcgcag acgattgccg aatacaattt gctggcgctg
      901 cccgtgattg atgatttggg cgagattgct ggaatcgtca cggttgacga cgcgatggag
      961 attctgctgc cgaaaaatct cgaccgccgt ttgcctcgcc ttttcggata attactaaaa
     1021 aattcaccac ggaaacacag agatttcgaa agaaattaac agcgatgaag aggataaaaa
     1081 gggattttta aagacaacag tcaaaaggta aaattaagat tttacttttg cagttttctt
     1141 atctcgttca tcttttaaaa acgggaaaat cccgagcgta cggcaaacgt ttcggcgata
     1201 atttccgaac cgatgaaacc tttgcgattt gtcggcgtaa aacgtcataa gcttgtcgtt
     1261 aaagccgtcg gcttgataaa ctatcaggcg tcgcattcat tctaaaaaag gagtaaaaat
     1321 ttatgaagcg taaaagtatt ctgtttgtcc tggctctgtt tgcgttcgtg tcgctggcaa
     1381 atgtcggcag cgccggagat ttcgcggtcg gaacggtaat ggaagatttt aaattgaccg
     1441 acattaacgg agccgagcag tctttcggca aactgaaagg cgagaaggga acgattctgg
     1501 tttttctttc ggcgcagtgc ccggtcgtca aaaactacaa cgcgcgcatc tcggcttttg
     1561 ccaagagcta caaggcgaaa ggcatcaatg tcgtcggcat caattcaaac gctacggaat
     1621 cgctcgaatg ggtgaaatcg cacgcgacgg aaaattacga ttttccgatg ctgattgaca
     1681 aaggcaacgt catcgccgac aaactcggcg caaacgtgac gccggaaatt tatttctttg
     1741 acgagaacaa caagcttctc tacaaaggcg cgattgacaa cgaccgttcc ggcggcaacg
     1801 tgacgaacca ttatttgcag gacgcggtgg acgcgaaaat ggcgggcaag gcgattgcca
     1861 aaacttcggc aaacgctttc ggctgctcga ttaaacgcgc cgcggcgaaa aattaagcga
     1921 gtatgaaaaa ttccgtttgg gcgaaagcga aaattgtaat tcttttttcg gggctgattt
     1981 tcgttttcgc ccaaattttt tccgtcgagc ttcaagctca gaaacgccgc aaaacttctc
     2041 ctgcgcgaac cgccaaaaag aaaactccgg caaagcgcgc ggcgctccgg ttaaatctgc
     2101 cgaaagtcac gcagattgac acgactgcgc tgcaaagcct tttaaagcgc gacggcgcca
     2161 acgccaagcc tctgctcgtc aatttctggg cgacgtggtg cgacccgtgc cgcgaggaat
     2221 ttcccgattt ggtacaaatc gacgccgatt acaaaggaaa aatcgacttt ataaccatct
     2281 cgctcgatta tctttcggaa atcgacggcg acgtgccgaa atttctggct gatatgaaag
     2341 ccgctatgcc cgcttatctg ctgaaaaccg aaaacgaaag cgaagcgatt gcgatggttt
     2401 caaaagactg gcagggcggc ttgccgttca cgattctgtt tgacgcgagc ggcaaagaaa
     2461 tctacgtgcg gcagggaaaa atcaaagtcg aagccgtgcg cgccgagatt gataaagctt
     2521 taaatccgcc ccagccggaa agcgcttttg aaaagggaaa actcgacgcc gaaaaagacc
     2581 tggcagcggg aaaaataatt atcaagacta atttcatatc gcggcggcgc agagatgccg
     2641 gcgcagaata cggaataacg atagagccgg tactttctta taaagcacag gaaatcgagt
     2701 atctaaaagg ttataattca aaatccgaag cggaaatctc gaaaagattc ggagaagaag
     2761 tcttgaaaaa aattaaacct caggtaccgc cgccgaatta tccggctgat gtcgtcgaat
     2821 taaagctcgt ccttcccggt cagaaaataa attaattttt gccgaaaatt tgatggcgtt
     2881 ttagtagttt cgctgtgaaa tctgtgatac agtgtttcac tgctgcaata caaatccaaa
     2941 cttgaaaagg tgaaaaacta tgtttcgtaa aatcgaagat ttccggaagg cttgggagta
     3001 tgaaaccgaa tcgaccatta agcttcttaa ccatttaacc gacgaatcgc tgaatcagaa
     3061 agtgaccgaa aacgggcgct cgctcggatt tctggcgtgg catctggtga cgacgctggc
     3121 ggaaatgccc ggacacgtcg ggctgaaaat cgacgcgccg ctcgaaggcg aaacgccgca
     3181 aaccattgcg gaaatcgctt ccgcctttga aaaaggcgcg aaatccgtca gcgaggaagt
     3241 cgggaaaaat tggacggacg agactctgct gcaagaagat aatctctacg gcgagacgtg
     3301 gacgcgcggc gaaacgctcg gatatttgct ggttcaccag acgcatcatc gcgggcagat
     3361 aaccgtcctg atgcgccagg ctggcttgcc ggtttcgggg atttacggtc cggcgaaaga
     3421 agaatggcag gcaatgggaa tgccggcgat ggcttaaatt taccgtaaga attttcggat
     3481 gaaaggcgcg cagtctcaga ctttgcgcct ttcttttttg cgcttgattc gacacgcaaa
     3541 atataaaaag ttaaaatgtc tgtttcattt tctacaccgg aaataaactt aatgaccgaa
     3601 acaaaaatca cgcctgaaat cgtcgcgcag cacaacctga ctgccgaaga atacgagaaa
     3661 attctgaaaa taatggaacg cgagccttcg atgaccgagc tcgggatttt ttccgtaatg
     3721 tggtcggagc attgcagtta caaatcctcg cgcgttcatc tgaagcggtt gccggtgacg
     3781 gggacgcgag tcatcgttcc gccgggcgaa aacgcgggcg tggtcgatat cggcgacgac
     3841 tggtgcgtgg ctttcaaggt cgaatcgcac aatcacccgt catttatcga gccgtttcaa
     3901 ggtgcggcaa cgggcgtcgg cggaattttg cgcgatgttt ttacaatggg cgcgcgcccg
     3961 atcgcggcga tgaacagctt gcgtttcgga cctttgaacg aagaaaaaaa cggcagacgc
     4021 aacaaaacta ttttaaaagg ctgcgtagac ggcatcgcgc attacggcaa ttgcttcggc
     4081 gttccgaccg tcggcggcga agtcgttttc gacgaagctt atagtctgaa tcctctggtt
     4141 aacgcttttg ctctcggcat cgtgcgcaaa gaccaaatct ttttcggcaa ggcgtcgggc
     4201 gtcgggaatc cggttttgta tgtcggcgcg aaaaccggac gcgacggcat tcacggcgcg
     4261 acgatggcga gcgccgaatt tgacgacgaa gcgctggaaa aacgcccgac cgtacaggtc
     4321 ggcgacccgt ttctggaaaa gcttctgctc gaagcgtgtc tggaagcgat gcgctcgggc
     4381 gcgatcgagg gaatccagga tatgggcgca gccggcttga cgtcttcttc ggtcgagatg
     4441 gcggcgcgcg ccggaaccgg cttggagctt gatttaacgc tggtgccgca gcgcgagacc
     4501 ggaatgacgg cgtatgaaat gatgctgtcc gaatcgcagg aacgaatgct catcgtcgct
     4561 cacaaagggc gcgaaaaaca gattgtcgag attttcaata aatgggattt ggacgcggtc
     4621 gtcgtcgggc gcgtcgtcga aggaaaccgg ctgaaagttt tccataacgg aaatctcgaa
     4681 gccgatttgc cggtcgatgc cgtcaccgac gaagcgccga aatataatcg tccgatgctc
     4741 gcccgaattc aaaattcaaa attcaaaatt caaaattacg aagcccggca gccgaagacg
     4801 aaagaccaca ccgaagcgtt gaaaatgctt ctcgccgcgc cgaatatttg ctcgaagcat
     4861 tgggtttacg agcaatatga ccatatggtt cggacaaata cggcgatttt gcccggcgcg
     4921 gacgcggcgg tcgtgcgcgt caaggaaacg cggcgcgcca tcgc
//
LOCUS       DASWNE010000457         3489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_546428, whole genome shotgun sequence.
ACCESSION   DASWNE010000457 DASWNE010000000
VERSION     DASWNE010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546428"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2758
                     /locus_tag="VGB68_09150"
     CDS             <1..2758
                     /locus_tag="VGB68_09150"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049437.1"
                     /translation="LLAAFFSSNGQTPTPTATPSFAPAPQTSPTPATSPTPKSSDPLK
                     LFQYRQIGPHRGGRVAAVAGAASQPNVYYFGATGGGVWKTIDSGVNWEPISDDYFKTG
                     SVGAIAVAESDPNVVYVGMGEETVRGNVSHGDGVYKSPDAGKTWKFVGLGDTRQISRV
                     RIHPKNADIAYVAAIGHLWAPNAERGIFRTKDGGKTWQKILFRDASTGAIDLVFDPSN
                     PNVLYAAMWQVRRAPWGFESGGAGSSIYKTTDGGDTWTEISRNKGLPAGVLGKIGLTV
                     SPVNTQRIWAMVEAKDGGLYRSDDGGENWQRMSNNPQTMQRPWYYFRIYADTQNADTV
                     YVLNVGFHKSADGGRTFTTINVPHSDNHDLWIAPDNNQRMINGNDGGANVSLDGGRNW
                     TEQDQATAQFYRVALDQDFPYNIYGAQQDNSTIRIASRTSEFSIGEQHWYDVGGGESG
                     WIAPHPENSDIIFAGSYGGYLTRYDHRSKQLRTVNVYPDNPMGAGAEAMKYRFQWNYP
                     ILFSPHKSANGKYALYAGGNILFRSFDEGQSWQAISPDLTRDDKSKQVSTGGEITKDN
                     TSVEYYSTIFTVSESPVTAGVIWTGSDDGLVYVTRDGGGKWENVTPKGMPEWIQINSI
                     DASPHDAGTAYVAATAYKTDDYRPYLYKTTDYGKSWKKIVGGIPDDAFTRVVREDPNR
                     KGFLYAGTETGMYFSAGDGENWRSLQLNMPIVPITDLAVHKREKDLVVATQGRSFYVL
                     DNLTLLYQMTEAQRGEAFLFKPEDAYRSAGLGGGFQFPKSVPLGANPPNGAVVNYYLK
                     NKPKEVSLEFLDASGKVLRKFSGKPPAEGASAPQQAPGAEPNLPTEIGLNQFVWNYRL
                     PNAATVPGLILWGGSLAGPKIVPGNYQVRFSVDGKVIGSESFAVKSDPRLPTTAEDLQ
                     KQFY"
     gene            2759..3193
                     /locus_tag="VGB68_09155"
     CDS             2759..3193
                     /locus_tag="VGB68_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049438.1"
                     /translation="MSNINRKLTATHEAILEIRDVRKQLEDLSARLKEPSQKDIRDKA
                     ADIIKKLTAVEEELIQTKIKSGQDALNYPIKLNNKLAALSSAVDSADYAPTNQSYDVY
                     NDLTARIDSQLATLARIKTDDITAFNRMFAEKNLPVITTRSR"
BASE COUNT          838 a          955 c         1005 g          691 t
ORIGIN      
        1 gctgctcgcc gctttttttt cgtcgaacgg gcaaacgccg acgccgaccg cgacgccgag
       61 tttcgcgccc gctccgcaga cgtcgccgac gcccgcgact tcgccgacgc cgaaaagttc
      121 agacccgctc aaactgtttc aataccgcca aatcggaccg catcgcggcg gtcgcgtcgc
      181 ggctgtcgcg ggcgctgcgt cgcagccgaa cgtttattat ttcggcgcga cgggcggcgg
      241 cgtttggaaa acgattgaca gcggcgtgaa ttgggagccg atttccgacg attattttaa
      301 aaccggctcg gtcggcgcca tcgccgtcgc cgaatcagac ccgaacgtcg tctatgtcgg
      361 aatgggcgag gaaaccgtgc gcggcaacgt ctcgcacggc gacggcgttt ataaatctcc
      421 ggacgccgga aaaacgtgga aattcgtcgg cttgggcgac acgcgccaaa tctcgcgcgt
      481 gcgaattcac ccgaaaaacg cggacatcgc ttacgtcgcc gccatcgggc atctctgggc
      541 gccgaacgca gagcggggaa ttttcaggac gaaagacggc gggaaaacgt ggcagaaaat
      601 tcttttccgc gatgcctcga ccggcgcgat tgatttggtt ttcgacccgt cgaatccgaa
      661 cgttctttat gcggcgatgt ggcaggtcag acgtgcgccg tggggttttg aatccggcgg
      721 cgcgggcagc tccatttaca agacaacgga cggcggcgat acgtggacgg aaatttcgcg
      781 caataaaggc ttgcccgccg gagttttggg aaaaatcggg ctgacggttt cgcccgtcaa
      841 cacgcagcga atctgggcga tggtcgaggc gaaagacggc gggctttatc gctcggacga
      901 cggcggcgaa aactggcagc gcatgagcaa caatccgcag acgatgcagc gcccgtggta
      961 ttactttcgc atttacgccg acacgcagaa cgccgacacg gtttacgtcc tgaacgtcgg
     1021 ctttcacaaa tcggcggacg gcgggcgcac gtttacgacc atcaatgttc cgcattcgga
     1081 caaccacgat ttatggattg cgcccgacaa caatcaacgg atgattaacg gcaacgacgg
     1141 cggcgcaaac gtctcgctcg acggcggcag aaactggact gaacaagacc aggcgaccgc
     1201 gcagttttac cgcgtcgcgc tcgaccagga ttttccgtat aatatatacg gcgcgcagca
     1261 ggacaactcg acgataagaa tcgcctcgcg cacgtctgaa ttcagtatcg gcgagcagca
     1321 ctggtatgac gtcggcggcg gcgaatcggg ctggattgcg ccgcatccgg aaaactcgga
     1381 cattatcttc gcgggcagtt acggcggcta tctgacgcgc tacgaccacc gctcgaaaca
     1441 gcttcgcacc gttaacgttt atcccgacaa cccgatgggc gcgggcgccg aggcgatgaa
     1501 atatcgtttc cagtggaatt acccgattct gttttcgccg cacaaaagcg cgaacggtaa
     1561 atacgcgctc tacgcgggcg gcaacatctt gtttcgctcc tttgacgaag gacaaagctg
     1621 gcaggcaatc agcccggatt tgacgcgcga cgacaaatcg aaacaagtct cgaccggcgg
     1681 cgagattacc aaagacaaca cgagcgtcga atattattcg acgattttca ccgtctcgga
     1741 atcgcccgtc acggctggcg tcatctggac gggaagcgac gacgggcttg tctatgttac
     1801 gcgcgacggc ggcggaaaat gggaaaacgt cacgccgaag ggaatgcccg aatggattca
     1861 gattaattcc atcgacgcct cgccgcacga cgccggaacg gcttacgtcg ccgctaccgc
     1921 gtataaaacg gacgactatc gcccgtattt atacaaaacg accgattacg gcaaatcgtg
     1981 gaaaaaaatt gtcggcggaa ttcccgacga cgctttcacg cgcgtcgtcc gcgaagaccc
     2041 gaaccggaaa ggctttctct atgcgggaac cgaaaccgga atgtattttt ccgccggcga
     2101 cggcgaaaac tggcgctcgc tgcagctgaa tatgccgatt gtgccgatta ccgatttggc
     2161 ggtgcataag cgcgaaaagg atttggtcgt cgccacgcag ggacgctcgt tttacgtgct
     2221 cgataatctg acgctgcttt atcagatgac cgaggcgcag cgcggcgagg cgtttttgtt
     2281 caagccggaa gacgcttatc gctcggcggg tttgggcggc ggttttcagt ttccgaaaag
     2341 cgtgccgctc ggggcgaatc cgccgaacgg cgcggtcgtc aattattacc tgaaaaacaa
     2401 accgaaagaa gtttcgctcg aatttctgga cgcgtccggc aaagtgctgc gcaaattttc
     2461 gggcaagccg ccggcggaag gcgcgagcgc gccgcaacaa gcgccgggcg cagaaccgaa
     2521 tctgccgacg gaaatcggct tgaatcaatt cgtctggaat tatcgtctgc cgaacgcagc
     2581 gacggttccg gggctgattt tgtggggcgg aagtctcgcc ggaccgaaaa tcgtgccggg
     2641 aaattatcag gtgcgttttt cggttgacgg gaaagtgatt ggaagcgaaa gtttcgccgt
     2701 caaatccgac ccgcgacttc cgaccacagc ggaagattta cagaagcagt tttattagat
     2761 gtcgaatatc aaccggaagc tgacggcgac gcacgaagcg attctggaaa tccgcgacgt
     2821 tcgcaaacag ctcgaagatt tgtcggcgcg tctgaaagag ccgtcgcaaa aggatattcg
     2881 ggacaaagcc gccgacatta taaaaaaact gacggcggtc gaagaagaat tgattcagac
     2941 gaaaataaaa tcaggacagg acgcgctgaa ttacccgata aaactgaaca acaaactagc
     3001 cgcgctttcg tcggcagtag acagcgccga ttatgccccg accaatcagt catacgacgt
     3061 ttacaacgat ttgacggcgc gcatcgattc gcagctggcg acgctggcgc gaattaagac
     3121 ggacgacata acggcgttta atcgaatgtt cgccgagaaa aatctgccgg tcattacgac
     3181 cagaagcaga taaaatcgaa agaaggtttc accgcggaga cgcggagttg cagagaaaga
     3241 aaaatacagg gataacagga tttacaggga taagaaagat attttctatt tatccctgta
     3301 aatcctgtta tccctgtaaa ttctctttta taaattctcc gcgtctctgc gactccgcgg
     3361 tttattttta tctccggttt cgacttattt tccggtcagt tcccgcgcca aatcctgcgc
     3421 gtcgtagagc tttcgcaaaa cttcgagaac ggcggcgctg tggacgccga cggcgcggct
     3481 gccttcttc
//
LOCUS       DASWNE010000458         3665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_685769, whole genome shotgun sequence.
ACCESSION   DASWNE010000458 DASWNE010000000
VERSION     DASWNE010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685769"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..604)
                     /locus_tag="VGB68_09160"
     CDS             complement(143..604)
                     /locus_tag="VGB68_09160"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HEY0049439.1"
                     /translation="MPESFASKFDRIKFNFFPAYRGTGARVIYIADDYRRIRVKIPLS
                     WRTRNYVGTIYGGSMYAGIDPIYMLMLIKILGRQYIVWDKAAKIRFKRPGREALFADF
                     LITADEIDEIRKRLATEKSVDRVYKVELVDKNGKAHAIIEKTLYITKRRED"
     gene            complement(723..1271)
                     /locus_tag="VGB68_09165"
     CDS             complement(723..1271)
                     /locus_tag="VGB68_09165"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY0049440.1"
                     /translation="MKEIISELQKITDDAKAKFGNLSPGQINWRPSAEGWSVGQCFEH
                     LIKTNKLFDDELDKIAAGNRKNSFWESWSPLTSIGGRFLIKSLKSDERKFKAPTRKIV
                     PPSEIGANIIEQFAEHQQEVIEKIKRTENVDWRKTVITSPFMKLMTYSFADGLQVVVE
                     HEKRHLRQAERVLQADGFPKEI"
     gene            complement(1324..1758)
                     /locus_tag="VGB68_09170"
     CDS             complement(1324..1758)
                     /locus_tag="VGB68_09170"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HEY0049441.1"
                     /translation="MKESLTAKSAKVFAKGRKEMDENSLSNLVINRALKVHTALGAGL
                     LESSYKECLVYELQLAGLLVEKEKALPLVYGTVKLECGYRVDLMVENKLIVEIKAVEA
                     INDVYLAQVLTYLKLSGCKLGLLINFNEVRLKDGIRRVVNRL"
     gene            complement(1755..>3665)
                     /locus_tag="VGB68_09175"
     CDS             complement(1755..>3665)
                     /locus_tag="VGB68_09175"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0049442.1"
                     /translation="MKLVEAIPTTWTKPEIACGVFGFLNERLGKGVVAAKDRPNFIAN
                     RIGVFSMMATIHEMIRMNFTPTEVDQITGKAIGHASSATFRTSDLVGLDVTAHVVDNL
                     YPAIPEDEDREVFVLPDLIKTLLEKKLLGDKTKGGFFKKSTGADGKREILELDLNTFE
                     YKPQTKTKFASLDAAKQVEDLNARLKTLVWGKDKVGEFLWNTSSRISRYAANRVPEIA
                     DTIVEIDDAIKWGFGWEIGVFEAWDAIGVEESVERMKAENQTVPANVEAMLAAGAKTF
                     YKEENGHRYFFDFATNAYKPIPARAGVIILKSLKDQGRIIRQNSAATLVDIGDGVACL
                     EFHSKMNAIGSDTMQMMKFAVEEIESNCTGLVIGNQGGNFSAGANLMLLMLAAQEEEW
                     DDINLIIHTFQQANMRLRYSAKPVVVAPFGLTLGGGCEISLHGDRVRAAAETYMGLVE
                     VGVGLIPGGGGTKEMTLRAMDAAKRTPDADAFAFIRKSFELLGMGKVATSAQEARAWG
                     FLRAEDSISMNKDRQIADAKEIVVALSKEGYVQPQPRTDIPALGESGLATLKLGLHMM
                     KRGGYISDHDALIGTKLAKIMCGGDLNHQTYVSEQHLLDLEREAFLSLCGERKTQERI
                     AYMLKTGKPLRN"
BASE COUNT          847 a          999 c          840 g          979 t
ORIGIN      
        1 ctgacttcca gatatttttt ttcgttttca gcggcgcagg cttttgaaaa ctcggtcggg
       61 acgcccgtag tcgggcggga accgcatccg gcggcaatca aaagcaaagc taaaataact
      121 aaactcgaaa aagttttcat aatcaatcct ctcttcgttt tgttatatat aaagtttttt
      181 caataatagc gtgagctttg ccgtttttat cgaccagctc gaccttataa acgcggtcga
      241 ccgatttttc cgtggcgagc cgtttcctga tttcgtcaat ctcgtccgcc gttattaaaa
      301 aatcggcaaa caaggcttcc ctgcccggac gcttgaaacg aatcttcgcc gctttgtccc
      361 agacgatgta ttggcgtccg agaattttta tcagcatcag catataaatc gggtcgattc
      421 ccgcatacat acttccgccg taaatcgtcc cgacataatt gcgcgtgcgc cacgacaaag
      481 gaatcttcac gcgaatccgg cggtaatcgt cggcaatata aataacccgc gcgcccgtcc
      541 cgcgataggc gggaaagaaa ttaaacttga tgcggtcaaa tttactggca aaactttcgg
      601 gcatagattt tcaaaaacct tcggaaataa accgccggcg aaggcggata aacgcggacg
      661 aagactgatt gacgacaaaa acacaaattt gcgctttcaa aataccggct ttcatcgagg
      721 gtttatattt ctttcggaaa accgtccgcc tgcaaaaccc tctcagcctg ccggagatgc
      781 cgcttttcgt gttccacgac gacctgcaag ccgtcggcga aactgtaagt catcagtttc
      841 ataaacggcg acgtgataac ggtttttcgc caatcgacgt tttccgtgcg cttgattttt
      901 tcaatcactt cctgttgatg ttcggcaaat tgctcgatga tattcgcccc aatctcgctc
      961 ggcgggacga ttttccgggt cggcgcttta aattttcttt cgtccgattt cagagattta
     1021 atcaaaaacc tgccgcctat ggaagttagc ggcgaccagc tttcccagaa cgaatttttg
     1081 cggtttccgg cggcgatttt gtcgagctcg tcgtcgaata atttgtttgt tttaatcaaa
     1141 tgctcgaaac attgcccgac gctccagcct tcggcgctcg gtcgccagtt gatttgcccg
     1201 ggcgaaagat tgccgaattt cgctttcgca tcatcggtaa ttttttgtaa ttcggaaatt
     1261 atttctttca tattttttta accgcaaagg gcgcaaagtt tcccgcaaag ggcgcaaagg
     1321 catctagagt ctattcacga ctctgcggat tccatctttt agtcttacct cattgaaatt
     1381 tattaataac ccaagtttac agcctgaaag ctttaaatag gttagaacct gagctaaata
     1441 aacgtcattt attgcttcaa ctgctttgat ttcgactatt aatttgtttt caaccatcaa
     1501 atctacgcga taaccgcatt caagtttgac tgtgccgtaa accagcggca atgctttttc
     1561 cttttcgacg agcaaacccg ctaattgaag ttcataaacc agacactctt tataagacga
     1621 ctccaacaat cccgctccta aagcagtatg cactttcaat gcgcggttta tcactaaatt
     1681 tgaaagcgag ttttcgtcca tctctttgcg tccctttgcg aaaacctttg cgctctttgc
     1741 ggttaaactt tccttcaatt tctcaaaggc ttgccggttt tcagcatata cgcaatgcgt
     1801 tcctgcgttt ttctttcgcc gcacaagctc aaaaacgcct cgcgctccaa atcgagcagg
     1861 tgctgttccg aaacgtaagt ctgatgattc aaatcgccgc cgcacataat tttcgccagc
     1921 ttggttccga taagcgcgtc gtggtcggaa atatagccgc cgcgtttcat catatgcaga
     1981 ccgagtttca aggtcgccaa gcccgattcg cctaaagccg gaatatccgt gcgcggctgc
     2041 ggctggacgt agccttcttt tgacaaggcg acgacgattt ccttggcgtc ggcaatctgg
     2101 cggtctttgt tcatcgaaat cgaatcttcc gctcgcaaaa atccccaggc gcgcgcttcc
     2161 tgcgccgacg tggcgacttt gcccattccc aaaagctcaa aactcttgcg aataaaggcg
     2221 aaagcgtcgg cgtcgggcgt ccgtttggcg gcgtccatcg cccgcagagt catttccttt
     2281 gtgccgccgc cgcccggaat caagccgacg ccgacttcga ccaaacccat ataagtctcc
     2341 gccgccgcgc gcacgcggtc gccgtgcagc gaaatctcgc agccgccgcc gagcgtcaat
     2401 ccgaacggcg cgaccacgac cggctttgcc gaataacgaa gccgcatatt cgcctgttga
     2461 aaagtgtgaa ttatcaaatt gatgtcgtcc cactcttctt cctgcgcggc gagcatcaaa
     2521 agcatcagat tcgcgcccgc cgaaaaattt ccgccctgat tgccgataac cagtccggtg
     2581 cagttgcttt cgatttcctc gacggcgaat ttcatcatct gcatcgtgtc cgagccgatg
     2641 gcgttcatct tcgagtgaaa ttccagacac gccacgccgt cgccgatatc gacgagcgtc
     2701 gcggcggaat tttggcggat aatgcgtccc tggtctttga gcgattttaa aatgataacg
     2761 cctgcccgcg ccggaatcgg cttgtaggcg ttggtcgcga aatcgaagaa ataacgatga
     2821 ccgttttctt ccttgtaaaa agtcttcgcg ccggcggcga gcatcgcttc gacatttgcc
     2881 ggaaccgttt gattttcggc tttcattctt tcgaccgatt cctcgacgcc gatggcatcc
     2941 cacgcttcaa aaacgccgat ttcccagccg aaaccccatt ttatcgcgtc gtcaatctcg
     3001 acaatcgtat cggcgatttc cggaacgcgg ttggcggcgt agcgcgagat gcgcgaagaa
     3061 gtgttccaca aaaattcgcc gactttatct ttgccccaaa ccagagtttt caggcgcgca
     3121 tttaaatctt cgacttgttt tgccgcgtcg agcgacgcga atttcgtttt cgtctgcggc
     3181 ttgtattcaa acgtgttcaa atcgagttcg agaatctcgc gcttgccgtc ggcgccggtc
     3241 gattttttga aaaagccgcc tttcgtttta tcgcccagaa gcttcttttc cagaagcgtt
     3301 ttgattaaat ccggcaggac gaaaacttcg cggtcttcgt cctcaggaat agccggataa
     3361 agattgtcga cgacgtgcgc cgtgacgtcc aagccgacca ggtcggaagt gcgaaacgtc
     3421 gcgctcgaag cgtgaccgat tgcctttccg gtaatctggt cgacttcggt cggcgtgaaa
     3481 ttcatccgaa tcatttcgtg aatcgtcgcc atcatcgaga aaacgccgat gcggttggcg
     3541 ataaaattcg gtcggtcttt cgccgccacg acgcctttgc ccagccgttc attgagaaat
     3601 ccgaaaactc cgcaggcgat ttccggtttt gtccacgtcg taggaatcgc ctcgacgagc
     3661 ttcat
//
LOCUS       DASWNE010000459         1602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_263488, whole genome shotgun sequence.
ACCESSION   DASWNE010000459 DASWNE010000000
VERSION     DASWNE010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263488"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1602)
                     /locus_tag="VGB68_09180"
     CDS             complement(<1..>1602)
                     /locus_tag="VGB68_09180"
                     /inference="COORDINATES: protein motif:HMM:NF016915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049443.1"
                     /translation="FPEGKGTHTRESKTTAGGWCGPKLNEPSQYNSNSPFTVDREGAE
                     INDQKIDPENPDVLSGSKTWDTSGDTIKSFVYTVTWSFKRCPAELEILDVEFAEHPYP
                     DFETWKKIEDEETVDSNMVRIRAKVVNYSSETKFPKIEFKELKENITLENGETSISIA
                     PGEIREVDYLWDTSGFAWVTGGFPMSRRRIKVEVTEKQTRQMTKEIVITPRPVVLAHG
                     LWADHTAWDGYDKFFYKAHSSIWRTFAVGKDPSNGRMNTGEKGTWKPANSIIENAEQL
                     GKQIEFVQKNMNAWHLDVVAHSMGGLITRSYINDLMKVAPDGKPTIEHLVMLGTPNMG
                     SPCANLMGFAFWSLGKPVKSLEQLRPKYLAGFNQAVRNRKGVKFSNLVGTSVPATCQS
                     SVSGDGVVEIPSAIWEIGHIRYSKNIHTALTSEEDFWRLVWRRLSESRLGNHKPDPKQ
                     YHAQASDFNRRSENPVQFLNTSLGAGAEERQDEIAAPENVKINLTKEVSLEPRQTIEF
                     DIPAAGNASAGSAVTFMTHPDVAATL"
BASE COUNT          302 a          432 c          434 g          434 t
ORIGIN      
        1 agcgtcgcgg caacgtcggg atgcgtcata aaagtaacgg cggaaccggc gctggcgttg
       61 ccggcggcgg gaatgtcgaa ttcgattgtc tggcgcggct ccagcgagac ttctttcgtc
      121 aggttgattt tcacgttttc aggcgcggca atctcgtcct gtcgttcttc ggctcccgcg
      181 cctaagctcg tgttgaggaa ttgcaccgga ttttcggagc ggcgattaaa atcagacgcc
      241 tgcgcgtgat attgtttcgg gtccggcttg tgattgccca ggcgactttc cgagagcctg
      301 cgccagacca gccgccaaaa atcttcttcg ctcgtcagag cggtgtgaat gtttttcgag
      361 taacgaatgt gcccgatttc ccagattgcc gacggaattt cgaccacgcc gtcgccggaa
      421 acgctcgatt gacaagtcgc cgggacgctc gttccgacca gattcgagaa cttgacgcct
      481 ttgcgattgc gcaccgcctg gttgaatcca gccagatatt tcgggcgcag ttgttcgagc
      541 gatttgacgg gtttgcccaa tgaccagaag gcaaaaccca tcagattggc gcacgggctg
      601 cccatattcg gcgtgccgag catcaccaga tgctcgatgg tcggcttgcc gtccggcgca
      661 actttcatca aatcgttgat gtacgagcgc gtaatgagtc cgcccatcga gtgcgcgacg
      721 acatcgagat gccaggcatt catatttttt tgcacgaact cgatttgttt tccgagctgt
      781 tcggcgtttt cgataatcga gtttgccggc ttccaggttc ctttttcgcc cgtgttcatc
      841 cgcccgttcg acgggtcttt gccgacggcg aacgttcgcc aaatcgaaga atgcgctttg
      901 tagaaaaatt tatcgtagcc gtcccaagcc gtatggtcag cccacagacc gtgagccaaa
      961 acgaccgggc gcggcgtgat gacgatttct ttggtcatct ggcgcgtttg tttttcggtc
     1021 acttcgactt tgattcggcg gcgcgacatc ggaaaaccgc ccgtgaccca cgcgaaaccg
     1081 gatgtgtccc acaagtaatc gacttcgcgg atttcgcccg gcgcaatcga aatgctcgtt
     1141 tcgccgtttt cgagcgtgat attttctttt aattctttaa actcgatttt cggaaatttg
     1201 gtttccgacg aataattaac gactttcgcg cggatgcgaa ccatgttgct gtcaaccgtt
     1261 tcttcgtctt cgattttttt ccacgtttcg aaatcgggat acgggtgttc ggcaaattcg
     1321 acgtctaaaa tttccagctc agctgggcag cgtttgaagc tccaggttac cgtgtaaaca
     1381 aaacttttta tcgtgtcgcc gctcgtgtcc caggttttgc tgccggaaag cacgtccggg
     1441 ttttccgggt ctattttctg gtcattgatt tccgctcctt cgcggtcaac ggtaaacggc
     1501 gagtttgagt tgtactgtga cggctcgttg agtttcggac cacaccaacc gcccgccgtc
     1561 gttttgctct cgcgggtgtg ggtgcctttt ccttcgggaa at
//
LOCUS       DASWNE010000460         2494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_227361, whole genome shotgun sequence.
ACCESSION   DASWNE010000460 DASWNE010000000
VERSION     DASWNE010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227361"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2494
                     /locus_tag="VGB68_09185"
     CDS             <1..>2494
                     /locus_tag="VGB68_09185"
                     /inference="COORDINATES: protein motif:HMM:NF026036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY0049444.1"
                     /translation="YVMPATGGVPKQLTFYPAKGPFTPRWGYDNQVYDWTDDGKKIIF
                     RSHRDSWTLAETRLYTVPVEGGNAEPLPMPESGAGDFSPDGTKIVYSPRARDFRPEKR
                     YGGGQASQLYIFDVNTGDAKLITPETPRASREPMWIGDAIYYVSDRDGHFNLYAYDTK
                     TNKSAQQTTEKTYDVRWASSDRKSRIVYELNGELQIFDTRARKSAPVSITVPTDAVSS
                     RPNRIPVANFIADVSLSPKGERVLFGARGEIFSAPAERGATRNLTQSSGTHDKFPRWS
                     PDGSRIAYISDKTGEEEIWIIQQDGLKPAEQITRGGKAMRYAPEWSPDGKYIAFSDKD
                     GKVYVLTLATKQFVQVADSPQGQIRDYTWSPRGNHLAFSMPEENGLSSIYVWSEIDRT
                     TKKVTDSLFNEFNPAWDAGGNYLYYLSVREFAPQISNIEFNFAGNRNVGIYALALRAD
                     VKHPFPNESDEVTITKEEPKLESGVPSLTPTPTPMPTQTPSPTPTPSGSPTPTPTPAM
                     PNPNAVLRIDFDGLGNRVARVPVDADNIGNLSANKNFLFYTTGSAFYYGRAGERQPTL
                     KIFSLKDRKETTLTEGAFGYALSEDGSKIIFPQAGNFVLMDASPLGLQGKKNVSTAGL
                     QYDRVPAEEWNQIFNEVWRRYRDFFYVENMHGYDWEALREQYRPLLQYVAHRSDLNYV
                     IAEMVAELAAQHSYIEGGDWQNPPRMRVALPGARFALDAAAGRYKISKIFGGQNEEDI
                     YRSPLTEIGVSAKVGDYLLAINGEELTRNSDPYQLLRNKADNPVQLTLNDRPTFEGAR
                     TITYRPVTDETNLIYLDWITKNRRKVEQLSGGR"
BASE COUNT          595 a          694 c          698 g          507 t
ORIGIN      
        1 ttacgtgatg ccggcgacgg gcggcgtgcc gaaacaattg acgttttatc cggcgaaagg
       61 acctttcacg ccgcgctggg gctacgataa tcaggtttac gactggacgg acgacggcaa
      121 aaaaatcatc tttcgctcgc accgcgattc gtggacgctg gcggaaacca gactttacac
      181 ggttccggtc gagggcggaa acgccgagcc tctgccgatg ccggaatcgg gcgcgggcga
      241 tttttcgccc gacggcacaa agattgttta ttcgccgcgg gcgcgcgatt ttcgccccga
      301 aaagcgttac ggcggcgggc aggcgagcca gctttatatt tttgacgtca acacgggcga
      361 cgcgaaatta atcacgcccg aaacccctcg cgccagccgc gagccgatgt ggatcggcga
      421 cgcgatttat tacgtctccg accgcgacgg tcattttaat ctctacgctt acgacacgaa
      481 aacgaacaaa tcggcgcagc agacgaccga aaaaacttac gacgtgcgct gggcttcttc
      541 ggacagaaaa agccgcatcg tctacgaatt aaacggcgag ctgcaaattt tcgacacgcg
      601 ggcgagaaaa tccgcgcccg tttcgataac ggttccgacc gacgccgtct cgagccgccc
      661 gaaccggatt ccggtggcga attttatcgc cgacgtttcc ctgagcccga aaggcgagcg
      721 cgttcttttc ggcgcgcgcg gcgaaatttt ttccgctccg gcggaacgcg gcgcgacgcg
      781 gaatctgacg caatcaagcg gcacgcacga taaatttccg cgctggtcgc ccgacggctc
      841 gcgcatcgcg tatatctccg acaaaaccgg cgaggaagaa atctggatta tccagcagga
      901 cggcttaaag cccgccgaac aaatcacgcg cggcggaaaa gcgatgcgtt acgcgcccga
      961 atggtcgccc gacggaaaat atatcgcttt cagcgacaag gacggaaaag tttacgtttt
     1021 gacgctggcg accaaacaat tcgtgcaggt tgccgattcg ccgcaaggtc agattcgcga
     1081 ttacacctgg tcgccgcgcg gcaatcatct cgctttttcg atgccggaag aaaacggtct
     1141 ttcctcgatt tacgtctgga gcgaaattga ccgcacgacg aaaaaagtga ccgattcgct
     1201 gtttaacgaa ttcaatccgg cttgggacgc cggcggcaat tatttgtatt acctttcggt
     1261 cagggaattt gcgccgcaaa tctcaaatat cgaatttaat ttcgccggaa accgcaacgt
     1321 cggaatttac gcgctggctt tgcgcgcgga cgtcaaacac ccgtttccga acgaaagcga
     1381 cgaggtgacg attaccaaag aagaaccgaa gctcgaatcg ggcgtcccgt ctttaacgcc
     1441 cacgccgacg ccgatgccga cacaaactcc aagcccgacg ccgacaccga gcggcagccc
     1501 gacgccgacg ccgactccag cgatgccgaa tccgaacgcg gttttgcgga ttgatttcga
     1561 cggtctggga aaccgagtgg cgcgcgttcc ggtcgatgcc gacaatatcg gaaatctctc
     1621 ggcgaacaaa aattttctgt tttacacgac cggctcggcg ttttattacg ggcgcgcggg
     1681 cgagcgccag ccgacgctga aaatattcag tttaaaagac cgcaaggaaa cgactttaac
     1741 cgaaggcgca tttggctacg cgctttcgga agacggttcg aaaatcattt ttccgcaggc
     1801 gggaaatttt gttttgatgg acgcttcgcc tttgggcttg cagggcaaaa agaacgtttc
     1861 gaccgccggt ttacagtatg accgcgttcc ggcggaagaa tggaatcaga tttttaacga
     1921 agtctggcga cgttaccgcg actttttcta cgtcgaaaat atgcacggct acgattggga
     1981 agcgctgcgc gagcagtatc gaccgctttt gcaatacgtt gcgcatcgtt cggatttgaa
     2041 ttacgtgatt gccgaaatgg tcgccgagct tgccgcgcag cactcttaca tcgaaggcgg
     2101 cgactggcag aatccgccgc ggatgcgtgt cgctctgccc ggggcgcggt tcgcgctcga
     2161 cgcggcggcg ggtcgctaca aaatctcgaa aattttcggc gggcaaaacg aggaagatat
     2221 ttatcgctcg cccctgacgg aaatcggcgt cagcgcgaag gtcggcgatt accttctggc
     2281 aatcaacggc gaagagctga cccgaaactc cgacccgtac cagcttttgc gcaacaaagc
     2341 cgacaatcct gtgcagttga ctttaaacga tagaccgact tttgaaggcg cgcggacgat
     2401 aacttatcga cccgtaacgg atgaaacaaa tttgatttat ctggattgga ttacgaaaaa
     2461 tcgacggaaa gtcgaacagc tttccggcgg tcgc
//
LOCUS       DASWNE010000461         2780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_685893, whole genome shotgun sequence.
ACCESSION   DASWNE010000461 DASWNE010000000
VERSION     DASWNE010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685893"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1172
                     /locus_tag="VGB68_09190"
     CDS             <1..1172
                     /locus_tag="VGB68_09190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0049445.1"
                     /translation="SGVLRGCDILCFSHDWTGDPLSKTHLMRVLSRDNRILWINAIAN
                     RMPTASSKDVSRIFKKLKAFTEPIREVEKNIFVLNPLAIPTYGSAAVRKFNQSFLLSQ
                     VKSAMRKLKFQKPLNMVFNPAAGLLAGRLGEESLIYYCVDEYTAFTGAANGLKEIEEE
                     LFRKSDLVVVSAEKLLESKRKYNPNTFLIRHGTDWRHFRKALDAETPVPDEIKDLPKP
                     LIGFHGLLADWVDFELIKKVAVHFKNGSIVLIGKIAVDAEQKIKILDGIDNVHFSGRK
                     PYADLPAFCKGFDVALNPFIVNDLTLAANPLKVREYLAAGLEVVSTDIPEVRILKHCR
                     IGKTHEEFIGQIEAALENPASRVQISDSIRQESWEAKIDELREVIEKAKSEKGEK"
     gene            1153..1797
                     /locus_tag="VGB68_09195"
     CDS             1153..1797
                     /locus_tag="VGB68_09195"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEY0049446.1"
                     /translation="MKKAKSEKGVSRRFRILSAVILLFLGWIFLAPFLAESLIVEKPL
                     ERADAILILGGSATFMERTQKAAELYRKGISTRILLTDDGGEAGWSQAERRNPKFVEL
                     ARKSLLEQGVPPTAIEILPATVAGTIDEARAFAEKAGAENLKSVLLVTSAYHTRRALW
                     TFKRVSVENNPEIGIESARTGIQTPPPYQWWLSVAGWNLVAGEYVKSAYYRVYY"
     gene            1860..>2780
                     /locus_tag="VGB68_09200"
     CDS             1860..>2780
                     /locus_tag="VGB68_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049447.1"
                     /translation="MSSSSSGNAKIWAQKQKEREKILRRQHGGKAIETRHYKTLLSIV
                     VVIVVALPLAVFGFYAYQNFVEEAALEFAGNTIVVRAGGDLQSALDKSKPGDTILIQA
                     GASFRGNFNLPAKAGNEFVTIRTSVNDAQLPPAGTRLDPKRYAAVLPKLSSPNNEPVI
                     NTETGAHHYRFVGIEFGGTKDGIGNIIKIGSTDEKRLEDLPHHIEFDRVYIHATSAQG
                     QRRGIAANGRFLKIANSHISDIKRKGEESQAIAVWATDGNIEIVNNYLEAAAENILFG
                     GAGSALGLIPSDCIVRDNWMNKPLNWREEGW"
BASE COUNT          698 a          684 c          784 g          614 t
ORIGIN      
        1 gctccggcgt cttgcgcggg tgcgacattt tgtgtttcag ccacgactgg acgggcgatc
       61 cgctttcgaa aactcacctg atgcgcgttt tatcgcgcga taatcgaatc ctgtggataa
      121 acgccatcgc caaccggatg ccgaccgctt cgtcaaaaga cgtttcgcgg attttcaaaa
      181 aattaaaggc ttttaccgag ccgattcgcg aagtcgaaaa aaatattttt gtgcttaacc
      241 cgctggcgat tccgacttac ggcagcgctg cggtcagaaa attcaatcag agttttttgc
      301 tctcgcaggt taagtcagcg atgcgcaaac tcaagtttca aaagccttta aatatggttt
      361 ttaacccggc ggcgggcttg ctggcgggca gactcggcga agagagtctg atttattact
      421 gcgtcgacga atacacggct tttacgggcg cggcaaacgg cttaaaggaa atcgaagaag
      481 agcttttcag aaaatcggat ttggtcgtcg tttcggcgga aaagctgctc gaatcgaagc
      541 gaaaatataa cccgaacact tttctgattc ggcacggcac ggattggcgg cattttcgca
      601 aagcgctgga cgcggaaacg ccggttcccg acgaaattaa agatttgccg aaaccgttaa
      661 tcggatttca cgggctttta gccgattggg tcgatttcga gctgataaaa aaagtcgccg
      721 tgcattttaa aaacggctca atcgttttaa tcggcaaaat cgccgtcgat gccgaacaga
      781 aaataaaaat tctggacgga atcgataacg tccatttctc cgggcgcaag ccttatgcgg
      841 atttgccggc tttttgcaaa ggtttcgacg tcgcgcttaa tccttttata gtcaacgatt
      901 taacgctcgc ggcaaatcct ttgaaagtgc gcgaatatct ggcggcggga ttggaagtcg
      961 tttcgaccga cattcccgaa gtgcgaattt taaagcattg ccgcatcggg aaaacgcacg
     1021 aggaatttat cgggcagatt gaagcggcgc tcgaaaatcc ggcatcgcgc gtgcaaatca
     1081 gcgattcgat cagacaggaa agctgggagg cgaaaatcga cgagctgcgc gaagtgattg
     1141 agaaggcgaa aagtgaaaaa ggcgaaaagt gaaaaaggcg tgagccgccg cttcaggatt
     1201 ttgagcgccg tcattttgct ttttctcgga tggatttttc tcgcgccgtt tttggctgaa
     1261 agcctgattg tcgaaaaacc tctggaacgc gccgacgcga ttttgattct cggcggttcg
     1321 gcgactttta tggaaaggac gcagaaagcc gccgagcttt accggaaggg aatttcaacc
     1381 aggattttat tgaccgacga cggcggcgag gcgggctggt cgcaggcgga gcgcaggaac
     1441 ccgaaatttg tcgagctggc gcgaaaaagc ttgctcgaac agggcgtgcc gccgaccgcc
     1501 atcgaaattt taccggcgac ggtggcggga accatcgacg aagcgcgggc gtttgccgaa
     1561 aaagccggag ccgaaaatct gaaatccgtt ctgctcgtca cgagcgcgta tcacacgcgc
     1621 cgcgcgcttt ggacttttaa aagagtttcg gttgaaaata atccggaaat cggcatcgaa
     1681 tcggcgcgga cgggaattca gacgccgccg ccatatcagt ggtggctttc ggttgcgggc
     1741 tggaatctgg tggcgggcga atatgtgaaa agcgcgtatt accgagttta ttattaaaac
     1801 aagcgagacg ggcgcgcggc aaaagggacg gttattttta tttcttcgga gcgaagcgta
     1861 tgagcagcag cagcagcggt aacgctaaaa tctgggcgca gaagcagaaa gagcgcgaaa
     1921 aaattctgcg gcggcagcac ggcggcaaag cgattgaaac gcgacattac aaaacgcttt
     1981 tgtcaatcgt cgtcgttatc gtcgtcgcct tgccgctcgc cgtattcggc ttttacgctt
     2041 accaaaattt tgttgaggaa gcggctctgg aatttgccgg aaatacgatt gtcgtccgcg
     2101 cgggcggaga tttgcagtcg gctctggaca aatcgaaacc gggcgacacg attcttatcc
     2161 aggcgggcgc gtcgtttaga ggcaatttca atctgcccgc caaagcggga aacgaattcg
     2221 tcacaattcg cacttccgtc aacgacgcgc agctgccgcc cgccggcacg cggctcgacc
     2281 cgaaacgcta cgccgcggtt ttgccgaagc tttcctcgcc gaacaacgag cctgtgataa
     2341 atacggaaac cggcgcgcat cattaccgct ttgtcggcat cgaattcggc ggcacgaaag
     2401 acggcatcgg caacatcatc aaaatcggct cgaccgacga aaaacgcctc gaagatttgc
     2461 cgcatcacat cgaatttgac cgcgtttata ttcacgcgac ttccgcccag ggtcaaaggc
     2521 gcggcatcgc cgccaacggg cgctttttaa aaatcgcgaa cagccacatt tccgacatca
     2581 agcgcaaagg cgaggaatcg caggcgattg ccgtctgggc gacggacggc aacatcgaaa
     2641 tcgtcaataa ttatctggaa gcggcggcgg aaaatattct tttcggcggc gcgggttccg
     2701 ctttgggctt gattccgtcc gattgtatag tgcgcgataa ttggatgaat aaacccttga
     2761 actggcgcga ggaaggctgg
//
LOCUS       DASWNE010000462         4338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_156752, whole genome shotgun sequence.
ACCESSION   DASWNE010000462 DASWNE010000000
VERSION     DASWNE010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156752"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..569
                     /locus_tag="VGB68_09205"
     CDS             36..569
                     /locus_tag="VGB68_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049448.1"
                     /translation="MKNKSEAGFSYIDVMIAVVILLVGILALVSAITGAIFQTKGQEQ
                     QLTAKQIGSSTMESIMSVKETDAARLGWNKIGNICVTTPCPVPQGIFQNGVQPVKMDA
                     GPDEVVGTADDTGSVMTGFTRQIIIRDECDQDRPSYNCTPAGNMAVRIRSIEININYY
                     VGAIQRQERLTTVLTNY"
     gene            589..1686
                     /locus_tag="VGB68_09210"
     CDS             589..1686
                     /locus_tag="VGB68_09210"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY0049449.1"
                     /translation="MQNNLNSPNAEKTVVVRRNEKGFSLLELVIAMVIFIIVTVAIYG
                     VLQLAQRNRLLVNQQVQSTKNVRFALNLIGRDTYNAGYGYPMNSTVVLPDNKISSLLN
                     IPNDFDTTRDTVPPIIAGNDINANTFNSTANTKTDQVTFLFKDSSFNLIGAAGSEVSQ
                     PLNINAATTNATGIDEIVPISGSNAACRKNDIYLVTGNTGSALALVTALSGFDKVQFS
                     NGDVLGFNQTGSTGTLRGITTPASMQRIRMITYFVTVDGILTRREYANVPPVGTNPPQ
                     NWVDEPIVYGVENFQIKYVMDDGTLSDNPGAGADGIPGTADDLPANLAKIRQVRFTVH
                     VKTNELDHRNQPYRINMSSTFSTRNLGYDAS"
     gene            1710..>4338
                     /locus_tag="VGB68_09215"
     CDS             1710..>4338
                     /locus_tag="VGB68_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX N-terminal domain-containing
                     protein"
                     /protein_id="HEY0049450.1"
                     /translation="MNTLETAQNDFFSNGNAARQKGSALVIAVFVLALISIFVTLALT
                     RTAIEAAAVGNETAEGRTFYAAQGSLELMTRNFNKTFEVKINPSDADLAAVKTALVPR
                     LWEDYTFKPELDKTSNSMTVVLPGGEFAGLYAIRDNWRLRTTATDASGVQVQLTRNIL
                     NNRIPIFQFGIFYDDDLELYRPPRFGFGGRVHSNHHFFISPGSEGVYFDSRVTAAGQI
                     ITQSWRNGYTGDSGNNQTYIKNASGVWKQLLPTKGSALNTTSGASNNIFAGNPDLPPS
                     QLNTNWTTDNAIFDGNLQSGVKELKLPLAVGVDTDLVEMVKRGKKVGDIHNKNGVVTA
                     VAPADVDNPIMTSERYANKPGIRISLSDSKAKLPGCATGTGLTAVTGDCGIRLDGDWE
                     GKGNDPDRTNSTLWKRSRGYLPLAMSGGYQATRVNGERLYTGQQAWIKVETVEYDAST
                     ETVVTKDITQEFLSLGVTEQAPEVVEIDNYDETPPSSSLTATTAQSPSTGTDSRSIIK
                     LQRFAIPGAPIPGATNINSGVPSGSTRYLYPFSNYNFVLRYLGATSNIGNGCTSGCTA
                     VNDDPSAGANYENMGHLKAADYLGLVRSALVPFPIQMYDTREGTYYDDPSIYTANRVT
                     QNGVISMVDIDVANLRRFLRGDFDGTFPGNTPFANGNGTNTLRKNHIPDNSGWVLYVS
                     DRRGDYDFDGEYDMEDIYGANPGNDGVRQPGEDLQRPGSYGYGILNTDYTNEATKYAN
                     LWFPDYAAVIEHKFYRRGVRLINGEKLPGIYDSANAGNTKGFTVASENGIYVQGDYNA
                     TGVANKYTTGNTPYNEYLPFNSTSHIPASIVADSITILSNSWNDAKSFAYPYDLSQRK
                     ASETTVRFAMISGDTIANIE"
BASE COUNT         1117 a         1200 c         1122 g          899 t
ORIGIN      
        1 ctttgtagct tattaattcg agcgacggaa aaaatatgaa aaacaaatca gaagccggat
       61 tttcgtatat cgacgtgatg attgccgtcg tcatcctgct ggtcgggatt ttagcattgg
      121 tctccgccat tacgggcgcg atttttcaga ccaaagggca ggagcagcaa ttgaccgcca
      181 agcagatagg cagctcgacg atggaatcaa ttatgtcggt taaggaaacc gacgcggctc
      241 ggctcggctg gaacaaaatc gggaacattt gcgtcaccac gccgtgtccg gtgccgcaag
      301 gcatttttca aaacggcgtc cagcccgtca aaatggatgc cggtcccgac gaggtcgtcg
      361 gaaccgccga cgatacgggc agcgtgatga ccggatttac gcgccaaata attatcaggg
      421 acgagtgcga ccaggacaga ccttcttaca actgcacgcc tgccggaaat atggcggtca
      481 gaatccgttc aatcgaaatc aatattaatt attatgtcgg cgctattcag aggcaggaac
      541 gcctgacgac agttttgaca aattattaac cgccggaacc gaaaagctat gcagaataat
      601 ttaaattcgc cgaacgcaga aaagactgtt gtcgttcgcc gtaacgagaa aggattttcc
      661 ctgctcgagc tggtcattgc gatggtcatt tttatcatcg tgaccgtcgc catttacggc
      721 gttttgcagc ttgcgcagcg caatcgtctg ctcgtcaatc aacaggttca gtcaaccaaa
      781 aacgttcgtt ttgccctcaa cctgatcggg cgcgacactt acaatgccgg ttacggctat
      841 cctatgaaca gcacggtcgt gctgccggac aataaaattt cgagcctgct caatattccg
      901 aacgacttcg acacgacgcg agacaccgtg ccgccgataa tcgccggaaa cgacatcaac
      961 gccaacactt ttaattccac ggcgaacaca aagaccgacc aggtgacttt tctttttaag
     1021 gattcgagct ttaatctcat cggcgcagcg ggcagtgaag tttcgcagcc gctgaacatc
     1081 aacgcggcga cgaccaacgc gacgggcatc gacgaaatcg ttcccatttc gggaagcaac
     1141 gcggcgtgcc ggaaaaacga tatttatctc gtcaccggaa acaccggctc ggcgctggcg
     1201 ctggtgacgg ctttgagcgg cttcgacaaa gttcagtttt ccaacggcga cgtgctcgga
     1261 ttcaaccaga ccggctcgac cggcactctg cgcggaatta cgacgccggc gagtatgcag
     1321 cgcatccgga tgataactta tttcgtcacc gtcgacggca tcctgacgcg gagagaatac
     1381 gccaacgtgc cgccggtcgg aaccaatccg ccgcaaaact gggtggacga accgatagtc
     1441 tacggcgtcg aaaattttca aatcaaatat gtgatggacg acggaactct ctcggataat
     1501 ccgggcgccg gagccgacgg aattcccggc accgccgacg atttgccggc gaatctggct
     1561 aaaatccggc aggtcagatt tacggttcac gtcaaaacaa acgaattaga ccaccggaat
     1621 cagccttatc gaatcaatat gtcttccact ttcagcacac gtaatttagg ctacgacgcc
     1681 agttgaaaac agatgaggta gtaacagata tgaatacatt agaaactgcg caaaatgatt
     1741 ttttcagtaa cggaaacgcc gctcgtcaga agggttcggc tctggttatc gcggtctttg
     1801 ttttagctct catcagtatt ttcgtcacgc tggcgctgac cagaaccgcc atcgaagcgg
     1861 ctgccgtcgg aaatgaaacg gcggaagggc gaacttttta cgcggcacag ggcagcctgg
     1921 aattgatgac cagaaacttc aataaaactt ttgaagtcaa aatcaatccg tcggatgcgg
     1981 atttagccgc cgttaaaacc gctctggtgc cgcgcctgtg ggaagactac acatttaaac
     2041 ccgagctcga caaaacgtcc aacagtatga cggtcgtttt gccgggcgga gaattcgccg
     2101 gtttatacgc tatcagagat aactggcggc tgcggacgac ggcgaccgac gccagcggcg
     2161 tgcaggttca attaacgcgc aacattttaa ataatcgcat tcctatcttt caattcggta
     2221 ttttctacga cgacgatttg gagctttatc gcccgcccag attcggcttc ggcggtcgcg
     2281 ttcactcgaa ccatcacttt tttatttcgc ccggaagcga aggcgtatat ttcgattcgc
     2341 gcgtgactgc cgccggtcaa atcatcacgc agtcgtggcg caacggatac accggcgaca
     2401 gcggcaacaa ccagacttat atcaaaaacg cttcgggcgt ctggaaacag cttctgccga
     2461 ccaagggcag cgccttaaac acgacttcgg gcgcttccaa taatattttt gccggcaatc
     2521 ccgatttgcc gccgagccag ctcaacacca actggacgac cgacaacgcg attttcgacg
     2581 gcaatctgca atcgggcgtc aaggaattaa agcttccgct tgccgtcggc gtcgataccg
     2641 atttagtaga aatggtcaaa cgcggcaaaa aggtcggcga catccataat aaaaacggcg
     2701 tggtcacagc cgtcgcgccc gccgatgtcg acaacccgat tatgaccagc gagcgatatg
     2761 ccaataaacc gggcattcgc atcagtttat ccgacagcaa agccaagctt ccgggctgcg
     2821 ctacgggcac cggactgacc gccgtcaccg gcgactgcgg cattcgtctg gacggcgact
     2881 gggaaggaaa aggcaacgac ccggaccgaa ccaactcgac gctctggaag cgctcgcgcg
     2941 gctatctgcc tctggcgatg agcggcggct atcaggcgac gcgcgtcaac ggcgagcgtt
     3001 tatacaccgg acagcaggcg tggattaagg tcgaaacggt ggaatacgat gcctcgaccg
     3061 aaacggttgt aacaaaagat attacgcagg aatttttaag cctcggcgtg accgagcagg
     3121 cgcccgaagt ggtggaaatc gacaactacg acgagacgcc gccgagcagc tcgctgacgg
     3181 cgaccaccgc gcagtcgcct tcgaccggaa ccgacagccg ctcaatcata aaattgcagc
     3241 gcttcgccat tccgggagcg ccgattcccg gcgcgaccaa tattaacagc ggcgttccga
     3301 gcggctcgac gagatattta tacccttttt cgaattacaa ttttgtcctc cgttatctgg
     3361 gagccacttc caatatcggc aacggctgca ccagcggctg cacggcggtc aacgacgacc
     3421 ccagcgccgg ggcgaattat gaaaatatgg gtcatctcaa agccgccgat tacctgggct
     3481 tggtgcgttc ggctctggtt ccgttcccga ttcagatgta tgacacgcgc gaaggaactt
     3541 attacgacga cccgagcatt tataccgcca accgcgtcac gcaaaacggc gtgattagta
     3601 tggtcgatat cgacgtcgcc aatctgagac gatttttaag aggcgatttc gacgggacgt
     3661 ttccgggcaa cacgccattc gccaacggca acggcacgaa cactctccga aaaaatcata
     3721 ttcccgacaa tagcggctgg gtgttatacg tctccgaccg tcgcggcgat tacgattttg
     3781 acggcgaata cgatatggaa gacatctacg gcgcaaatcc gggaaacgac ggcgtcagac
     3841 agccgggcga agatttgcag cgacccggaa gctacggcta cggaatcctg aataccgatt
     3901 acaccaacga agcgaccaaa tacgccaacc tctggtttcc ggattacgcc gccgtcatcg
     3961 agcataaatt ctaccggcgc ggcgtccgtc tgattaacgg agagaaatta ccgggcattt
     4021 acgattcggc taacgcgggc aatacgaaag gctttaccgt cgcttccgaa aacggcatct
     4081 acgtccaggg agactacaac gcgaccggcg tcgccaataa atacacgacc ggcaacacgc
     4141 cgtataatga atatctaccg tttaacagca cgagtcatat ccccgcttcg attgtcgccg
     4201 attcgataac cattctttcc aatagctgga acgacgccaa aagcttcgct tatccgtacg
     4261 atttgagcca gcgcaaggcg agcgaaacga ccgtccgttt tgcgatgatt tccggcgata
     4321 ccatcgccaa tatcgaag
//
LOCUS       DASWNE010000463         2151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_685929, whole genome shotgun sequence.
ACCESSION   DASWNE010000463 DASWNE010000000
VERSION     DASWNE010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685929"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..788)
                     /locus_tag="VGB68_09220"
     CDS             complement(207..788)
                     /locus_tag="VGB68_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049451.1"
                     /translation="MRKITFGRNSRLNKLFQLFAILLALCSAGFAQERTWKTFSPAGG
                     AWSILAPGELRPDAEAQESPSTKGSYSYSDFNGFFAVIYRDSPKRWVPWKPNYNAYFK
                     KIRKDAVEAAKGQLLKDEEFSSGDLTGREVYIKVPVGTLVGAEGLVITKYRVERLRMF
                     FREKRFYLLMAVLPEADVNSPEANNYFNSFTAK"
     gene            complement(838..1899)
                     /locus_tag="VGB68_09225"
     CDS             complement(838..1899)
                     /locus_tag="VGB68_09225"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0049452.1"
                     /translation="MKIIGAILFLMLLILPAELRAQTPQPDKQVRKGEKTLVSVPVTV
                     SDREGRYISGLKKEDFNLFEDGVKQNVAFFATEDEPISIALVLDTSGSTKDVLDKIKR
                     AAGDFIELLNPDDKCQIVTFDSQVRILNSFTSDKQMLAQSLDKARSATEDGTVLFRAV
                     EQVARQSFTDIQGRKVIVLLSDGKDFGSSLTKDELLSQLEELDVMIYTVFYQTGAGSA
                     KPAAAPNGIIREEKETPKISEKKTPKKKKGYTIFIPVPGDLYTEKDVKLTEKASGAEA
                     INSLKQMSEMTAGRFYLSETPDLGEIFKKIGGELRQQYRLGYYTKDASGDGVREIVVK
                     VGRTDAVVRARGKFRSKAL"
BASE COUNT          479 a          566 c          545 g          561 t
ORIGIN      
        1 cgcctttttg aacggtctgg attttttctg ccgttatctc gggcgctttt cgcagataag
       61 tgaaggtcac aatcacctct ccctgctttt gcgcaaacgt cgaaaatgca agcgacaaag
      121 tgaaaaccgt gatgataaaa aatttcataa gcgccttttt ccgcgattcg aacttccttg
      181 ctcccttgcg ccgaatcgcc gtgatgctat ttggcggtga aggaattgaa ataattgttt
      241 gcctccggcg agtttacatc cgcttccggc agcacagcca taagcaggta aaaccgcttc
      301 tcgcgaaaaa acatccgcaa tcgttcgacg cgatatttgg ttatcaccag accctcggct
      361 ccgaccagcg ttccgaccgg gacttttatg taaacttcgc gaccggtcaa atctccgctg
      421 gaaaattctt cgtctttgag cagctgacct ttggcggctt cgaccgcgtc tttacggatt
      481 tttttaaaat aagcgttgta attcggcttc catggaaccc agcgtttcgg cgaatcgcga
      541 taaatgacgg cgaaaaaacc gttgaaatca gaatacgagt aactgccttt cgtgctgggc
      601 gattcctgcg cctcggcgtc gggacgcagc tctcccggcg cgagaatgct ccacgcgccg
      661 cctgccggac tgaaggtttt ccaggttcgc tcctgagcga aaccggcgga acacaaagcg
      721 aggagaatcg caaacaattg aaataatttg tttaatcggg aatttcgccc gaacgtaatt
      781 tttcgcataa accgccagcc gggacgcgtt ttccggcgaa attctattta tctgttatta
      841 caaagctttc gaacggaatt ttccgcgagc gcgcacgacc gcgtccgtcc gcccgacttt
      901 caccactatc tcccggacgc cgtcgcccga ggcgtctttg gtgtaatacc cgagccggta
      961 ttgctgtctc agctcgccgc cgattttttt gaaaatctca cccaaatccg gcgtctcgct
     1021 caggtagaag cgcccggcgg tcatctccga catttgtttc agagaattaa tcgcttccgc
     1081 gcccgaagcc ttttccgtta atttaacgtc tttctccgtg tagaggtcgc ccggcacagg
     1141 aataaatatg gtgtagcctt ttttcttttt cggcgttttc ttctccgaaa ttttcggagt
     1201 ttctttttct tcacggataa ttccatttgg agcggcagcc ggtttagccg agcccgcgcc
     1261 ggtctggtaa aaaaccgtgt aaatcatcac gtccagctct tcgagctggc tcagcagttc
     1321 gtctttcgtc agcgagctgc cgaaatcttt cccgtccgag agcagcacga ttacttttcg
     1381 tccctggata tcggtgaacg attggcgggc gacctgttcg acggcgcgga acagaaccgt
     1441 cccgtcttcg gtcgccgagc gggctttgtc gagcgattgg gcgagcattt gcttatcgga
     1501 agtaaatgaa ttgagaatcc gcacctgcga gtcgaaggta actatctgac acttgtcgtc
     1561 cgggttcagc agttcgataa aatctccggc ggcgcgcttg attttgtcca aaacatcttt
     1621 ggtgctgccg ctggtgtcaa ggacgagagc gatgctgatc ggctcgtctt cggtggcgaa
     1681 aaaagcgacg ttttgtttta cgccgtcttc aaaaagattg aaatcctctt tcttcaagcc
     1741 ggagatgtaa cgcccttcgc ggtcgctgac ggtaaccgga acgctgacga gcgttttctc
     1801 tcctttgcgg acttgtttat ctggttgggg cgtttgcgcg cgcagctcag ccggcaggat
     1861 taaaagcatc aaaaaaagta tcgcgccgat gattttcata acgtttaaaa gtatagattt
     1921 atattgaggt gttggcaata aaaagacttg accgcggcgc ggtttccggc tttattccgg
     1981 gcttttctgc ggcagccatt gcacgcggcg tcaagcggac gaagcgacgg gatttgcgct
     2041 cgccgagccg cccgtgcaaa atcgaaggtg atttaataaa ttcatgaacg gaagcgcgcc
     2101 gcagcaaaaa atcaaatttt acaccacgag ctgcgcgggc agggagatta a
//
LOCUS       DASWNE010000464         2874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_327305, whole genome shotgun sequence.
ACCESSION   DASWNE010000464 DASWNE010000000
VERSION     DASWNE010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327305"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VGB68_09230"
     CDS             <1..255
                     /locus_tag="VGB68_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049453.1"
                     /translation="GRLVLSCLGTHKDLTENRVTLKEGMKLVFYTDDEDDEGKSDDLV
                     VEGTIERDAEKNRWVARINWDEIKNISRLSRAEKKQLGIA"
     gene            322..1695
                     /gene="lysC"
                     /locus_tag="VGB68_09235"
     CDS             322..1695
                     /gene="lysC"
                     /locus_tag="VGB68_09235"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HEY0049454.1"
                     /translation="MSERLNPTVMKFGGTSVGDVAAFERVFRIVSEQVDKKPVVVVSA
                     MTKVTDALLTAFDTAKKGDSEAANDFLEPHFARHEEVSRTFLNEKQQRAFDEELSFVK
                     GELRELFKRVSRRSLPLAMMKDAVVSYGEQLSSRLLAEVLKAKDIKARQMDSRRLIVT
                     DDEYGAAQPVWDETERLIKIELEYLIRNGEIPVMGGFIAASLSGETTTLGRGGSDYSA
                     ALVAAALRARELQIWTDVTGVMTCDPRIVSEARTIPVLSYEEAAELAYFGAKVLHPKT
                     IQPAVDYSIPVRVCNTFEPETVGTMVLSESGEAPNRIKSIAHKKNITILRISSARMLG
                     SYGFMSALFQIFERYRTVIDVISTSEVSVALTLDNTASLEQVVSDLKRLGDVEVEPDN
                     AVVCVVGEGLRAASGLAAKIFSTIEDINVGLVSHGASNVNLTFVVKEEVVSEVIKRLH
                     KEFFAGS"
     gene            1818..2816
                     /locus_tag="VGB68_09240"
     CDS             1818..2816
                     /locus_tag="VGB68_09240"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0049455.1"
                     /translation="MNLFELTKNLMNIPSVSGEEERVGFFLRDYLESLGWTVELQPVS
                     ENQNNVIAYLGESPRVFLSTHMDTVPPYLPPSEDDEKIYGRGACDAKGIIASQIFAAE
                     ELRKQGVRDIGLLYTVDEEQSSTGAKEANKHALAAKCEYLINGEPTDNDLAIGSKGSL
                     RFLIKTEGKAAHSAYPEMGESAIEKLLDILEDIRRIEFPNDEFFGATTNNIGTLNAGL
                     KINIVPAHAEAGLLIRLTTAKEPIWEILEKTIAGRGRLELMSYSLPVKMLAVEGFKQK
                     VVRFTTDIPHLPDWGTPLLLGAGSILVAHTKDEFVLKRDLEEAVGLYVNLVKKLLG"
BASE COUNT          737 a          695 c          803 g          639 t
ORIGIN      
        1 ggtcggctcg tgctgagctg tctcggaacg cataaagatt tgactgaaaa ccgcgtcacg
       61 ctcaaagaag gcatgaagct tgttttttac actgatgatg aagatgacga aggaaaaagc
      121 gacgacttag tcgtcgaagg aacaatcgag cgcgacgcgg aaaaaaacag atgggtcgcc
      181 cgcataaact gggacgaaat taaaaacatt tcccgcctat cccgcgcgga aaagaagcaa
      241 ttgggaattg cgtaaaatca aaaattaacc atcatagaaa cttgacaatt ccattagaaa
      301 aagttgaact tctcactaat tgtgagtgaa agattaaacc cgacagtaat gaaattcggc
      361 ggcacgtcgg tcggcgacgt cgccgccttt gagcgcgttt ttcgcattgt atccgagcag
      421 gtcgataaaa agcccgtcgt ggtcgtttcg gcgatgacga aagtgaccga tgcgcttttg
      481 acggctttcg acacggcgaa aaaaggcgat tccgaagcgg cgaacgattt tctcgaaccg
      541 catttcgcgc gtcacgaaga agtttcccga acttttctga atgaaaaaca gcagcgggct
      601 ttcgacgagg aattgagctt tgtgaaaggc gaactgcgcg agcttttcaa gcgcgtttcg
      661 cgccgctcgc ttccgctggc gatgatgaaa gacgccgtcg tctcctacgg cgagcagctt
      721 tcttcaaggt tgctggcgga agttttaaag gcgaaagaca ttaaagcgcg gcagatggat
      781 tcgcgccgcc tgattgtgac ggacgacgaa tacggcgcgg cgcagccggt ctgggacgaa
      841 accgagagat taatcaaaat cgagctcgaa tacctgattc gaaacggcga aatccccgtt
      901 atgggcggat ttatcgcggc gagcctgagc ggcgaaacga cgactctggg gcgcggcggc
      961 tcggattatt cggcggcgct cgtcgcggct gccttgaggg cgcgcgagct gcaaatctgg
     1021 acggacgtga cgggcgtgat gacgtgcgac ccgcgcattg tcagcgaggc gcgaacgatt
     1081 cccgttctca gctacgaaga agccgccgag ctcgcctatt tcggcgcgaa agttctgcac
     1141 ccgaaaacta ttcagccggc agtcgattat tcgattccgg tcagagtctg caatacgttc
     1201 gagccggaga cggtcggcac gatggttttg tcggaatccg gcgaagcgcc gaacagaatc
     1261 aaatccatcg cgcacaaaaa aaacatcacg attttgcgga tttcgtcggc gcggatgctc
     1321 ggctcttatg gttttatgtc cgcgcttttt cagatttttg agcgctaccg gacggtgatt
     1381 gacgtgattt cgacctcgga ggtctccgtc gctctgacgc tcgacaacac ggcttcgctc
     1441 gaacaggtcg tgagcgattt aaaacgcctc ggcgatgtcg aggtcgagcc ggataacgcg
     1501 gtcgtctgcg tcgtcggcga aggcttgcgc gccgcttccg gcttggcggc aaaaatcttt
     1561 tcgaccatcg aggacatcaa cgtcgggctc gtctcgcacg gcgcgtcgaa cgtcaacctg
     1621 acgtttgtgg tcaaagaaga agttgtgagc gaggtcatta aaagattgca taaagaattt
     1681 tttgccggca gttaaagcga cgaaaaaatt atgaaaggtt cggagcggaa tgaatccttt
     1741 tatcaggcgg tcgccgccga gatactaaaa tcgaattgct ttgagagaac ggcacaaggt
     1801 gctgtatcaa tagttttatg aatcttttcg aattaaccaa aaatttaatg aatattccgt
     1861 ccgtttcggg cgaagaagag cgggtcggat tttttctgcg cgattatctg gaaagcctcg
     1921 gctggacggt cgaattacag ccggtttcgg aaaatcaaaa caacgtcatc gcttatctcg
     1981 gcgaaagccc gcgcgttttt ctttcgacgc atatggacac ggttccgcct tacctgccgc
     2041 cgtccgaaga cgatgaaaaa atctacgggc gcggcgcgtg cgatgcaaaa ggcattatcg
     2101 cgtcgcaaat cttcgcggcg gaagaattga gaaaacaagg cgtccgcgac atcggattgc
     2161 tctatacggt tgacgaagaa caatcttcga ccggcgcgaa agaagcgaac aagcacgcgc
     2221 tggcggcgaa atgcgaatat ctcatcaacg gcgagccgac cgataacgat ttggcaatcg
     2281 gctcgaaagg ctccctgcgt tttctcatca aaaccgaagg caaagccgcg cattcggctt
     2341 acccggaaat gggcgaatcg gcgattgaaa agcttctgga tattttagag gacattcgcc
     2401 gaatcgagtt tccgaacgac gaatttttcg gcgcgacgac caataatatc ggcacgctca
     2461 atgccggttt gaaaatcaac atcgttccgg cacacgccga agccggtttg ctgattcgcc
     2521 tgacgaccgc taaagagccg atttgggaga ttctggaaaa aacgattgcc ggtcgcgggc
     2581 gtctggaatt aatgtcctac agtttgcccg ttaaaatgct cgccgtcgaa ggcttcaaac
     2641 aaaaagtcgt gcgctttacg accgacattc cgcacctgcc cgattgggga acgccgcttc
     2701 ttttaggcgc aggctcgatt ttggtggcgc acacgaaaga cgagtttgtt ttgaaacgag
     2761 atttggaaga agcggtcggg ctttacgtga atttagtcaa aaagcttctc ggttaaaatg
     2821 aaatattgag gtcatcgatt tgaaaatagc tttaatcggc tacggcgcga tggg
//
LOCUS       DASWNE010000465         4495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_263675, whole genome shotgun sequence.
ACCESSION   DASWNE010000465 DASWNE010000000
VERSION     DASWNE010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263675"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1226)
                     /locus_tag="VGB68_09245"
     CDS             complement(<1..1226)
                     /locus_tag="VGB68_09245"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY0049456.1"
                     /translation="MRKTVRAFLLFTFLFLNSSFAGAQNAPDWRAFDAFVNKTLADWE
                     APGAAIAIVKEDRVIYAKGYGVKEIAKNDKTDERTIFGIASNSKAFTATALAMLVDEG
                     KINWDDKVIKHLPDFRLADDYATREITIRDLLSHRSGLPAYGGDILWWGGDYGRNDIV
                     QRIRFVKPAYSFRSTYAYQNIPFIIAGQIIEKASGKTWDEFVKTRILQPLKMNETTTS
                     IKDFSPGANKTTPHFRDLETGKTFPIAWRNLDSGAAAAGINSNVLDAANWLRLQLNEG
                     EFEGRRLVSQKNIREIQSPQTIIPFANPGEPKLKSNFRAYGLGWSLREYAGYKMVTHN
                     GWTDGQLSMTAMIPDRRVGVVVLTNVHHQNVYAPLAYRALDVALGLSETDWNAYFFKF
                     IKDAETEIIAGEKKLEA"
     gene            1359..1682
                     /locus_tag="VGB68_09250"
     CDS             1359..1682
                     /locus_tag="VGB68_09250"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEY0049457.1"
                     /translation="MPDSSFLYRALLRATFFLLLIPAFARAEQLSVKTYTSADGLLYE
                     GIYRLYQDSRGFIWFATPIDISRFDGYRFTNYGMEDGLFNTLINDFVEDDNGVYWLGS
                     SSGSN"
     gene            1836..>4495
                     /locus_tag="VGB68_09255"
     CDS             1836..>4495
                     /locus_tag="VGB68_09255"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HEY0049458.1"
                     /translation="MFTLDDAQPDKKFSRVALDVPASENRSFPVYSLAEDAEGSLWVG
                     HERGLTRLLPDGRTATYEIRASGDAATSAFSLGFDNQNRLWIVTGKRTATVFKPEPAG
                     SVDFPNRAKRELRMETAFDSGNLRAGFAYAFKPAETQADGDFGAVYCARDGRIFFGSR
                     RKGLIEFDGANFRFYTRENGLSDNNVSFVREDSFGNLWLASSWGAMKITRRGFVTYNT
                     ADGLQADRISDLFTDRAGTIYAVNAGWNINQFDGRRFVSVRPNLPADLSNFWIHHITK
                     DSRDEWWIPTLKGLFRFPRVERVEQLATAKPIAVYTIREGLPTDSVLVTFEDSRGDIW
                     FSYENAPGRISRWERATETFHHYAPQNGIPEACRPFYYNEDAAGALIITCQFESYAIY
                     RGDGRFTAHSTPEMKKAGWLHRALVDRKGRFWIGTPLNGLLRYDNPTSENPTETVYLK
                     KDGLSSSHIQYLVEDLYGKIYYVTSSGMDRMDTETGEIKYYNVADGFPAAGTGVAMRD
                     AGGALWLGTSRGLSKFVPEPDKPPPPVFISSLRIAGNDVPVSVLGETQISDLTLAPDE
                     RQMQIDFYGLSLASGETLRYQYKLEGVSGEWSPPNEQRTVNYPNLTSGSYRFLVRAVN
                     PDGSFSAEPASISFTVLRPLWQRWWFLLLAALAVGAGVYAFYRYRIAQIIKLERVRTR
                     IATDLHDDIGSSLSQIAILSEVVRQKVGAGVAGEPLNMIAETSREMVDAMSDIVWAIN
                     PQKDYLNNLVQRMRRFAEDILDAQEISYRFNSLENLKDVSVGSDVRREAYMIFKECVN
                     NAVKHSGAKKIDFRINLENEFLVIEISDDGRGFDTEEAENLDSLGGNGLINMRRRADN
                     LGGTFLIDSAPGKGTRAVIRIPIVKKLFRV"
BASE COUNT         1002 a         1247 c         1215 g         1031 t
ORIGIN      
        1 gcttcgagct tcttttcgcc ggcgatgatt tcggtttcgg cgtccttgat aaatttgaaa
       61 aaatacgcgt tccagtcggt ctccgaaagt ccgagcgcga catccagagc gcgataggcg
      121 agcggcgcgt aaacgttctg atgatgaaca ttggtcaaaa cgacgacgcc gacgcgtctg
      181 tcgggaatca tcgccgtcat cgaaagctga ccgtccgtcc agccgttatg cgtaaccatt
      241 ttgtagcccg cgtattcgcg caggctccag cccagcccgt aggcgcgaaa attcgatttc
      301 agcttcggct cacccggatt ggcgaaagga attatcgtct gcggcgattg aatctcgcgt
      361 atgtttttct gcgaaacgag ccgtctgcct tcaaattcgc cttcgtttaa ttgcagccgc
      421 agccagtttg ccgcgtccaa aacattggaa tttattccgg ctgccgccgc gccgctgtcc
      481 agatttcgcc acgcaatcgg aaaagttttt ccggtttcga ggtcgcgaaa atgcggcgtc
      541 gttttgtttg cgcctggcga aaaatctttg atgctcgtcg tcgtttcgtt cattttgagc
      601 ggctgcagga tgcgcgtttt gacaaattcg tcccacgttt tgccggaagc tttttcgata
      661 atctgaccgg cgatgataaa cggaatattt tgataagcgt aagtcgagcg aaaactgtag
      721 gcgggcttga cgaaccgaat tctttgcacg atatcattgc gcccgtaatc gccgccccac
      781 cagagaatat cgccgccgta ggcgggcaag ccgctgcggt gcgaaagcaa atcgcgaatc
      841 gtgatttcgc gcgtcgcgta atcgtcggcg agccggaaat cgggcagatg ttttataact
      901 ttgtcgtccc aatttatttt tccctcgtcg accagcatcg ccagcgccgt cgccgtaaaa
      961 gccttcgagt ttgaggcgat gccgaaaatg gtgcgctcgt cagtcttgtc atttttggcg
     1021 atttccttta cgccgtagcc tttcgcgtaa attacgcggt cttctttgac aatcgcaatc
     1081 gccgcgcccg gcgcttccca atcagccaga gttttgttga cgaaggcatc aaacgcccgc
     1141 caatccggcg cattctgcgc gcccgcgaaa gacgaattta aaaacaaaaa ggtgaaaagc
     1201 agaaaagccc gaacagtttt tctcataaat aattcctcgc gaccgattca cagattacca
     1261 caaatttccc gcgaaaatct tacccggctt ttgcctgctg ccgtaaattg cattaacatt
     1321 ttctcgccct gtagtttctt tcaatcgccg gaaatcaaat gccagattcg agctttcttt
     1381 atcgcgccct tctgcgggcg acattttttt tactgctaat tccggcgttt gcccgcgccg
     1441 aacagctttc cgtcaaaact tacacgtcag ccgacggctt gctctacgaa gggatttatc
     1501 gtctttacca ggattcacgc ggttttatat ggtttgccac gcccatcgac atcagccgct
     1561 ttgacggcta ccgctttacc aattacggga tggaagacgg tctcttcaac acgctcatca
     1621 acgatttcgt cgaggacgac aacggcgttt actggctcgg ctcaagcagc ggcagcaatt
     1681 aaggcgcggc tatctaccgc tttgacccgc gccttaaaaa cagcgacgag aaaaaattta
     1741 ttcctttcaa catcagcgaa gaaactgcga cgaacgacgt tagccggctt tttaaaaaca
     1801 gtcgcgggca gattttcgcc gccgccgacg gcggtttgtt tacgctcgac gacgcgcagc
     1861 cggataaaaa attttcccgc gtcgcgctcg atgtgcccgc ttccgaaaac cggtcttttc
     1921 ccgtttattc gcttgccgaa gatgcggaag gaagcctctg ggtcggacac gagcgcggat
     1981 tgacgcggct tttgcccgac ggtcggacgg cgacttatga aatccgggct tcgggcgatg
     2041 cggcaacctc ggcattttca ctcggcttcg ataatcaaaa tcgcctttgg atagtgacag
     2101 gcaaacggac ggcgacggta tttaaacccg agcccgccgg ttcggtcgat ttcccgaacc
     2161 gcgccaaacg cgaattgaga atggagacgg cattcgattc cggcaatttg cgcgcgggat
     2221 tcgcttacgc cttcaagccc gcggaaacgc aagccgacgg cgatttcggc gcggtttact
     2281 gcgcgcgcga cggcagaatt tttttcggct cgcgcagaaa aggcttgatc gagtttgacg
     2341 gcgcgaattt ccgtttttac acgcgcgaaa acggtttgag cgacaacaac gtctcgttcg
     2401 tccgcgagga tagtttcggc aatctctggc tggcgtcgag ctggggcgcg atgaaaatca
     2461 cgcgcagagg ttttgtcact tacaacaccg ccgacggtct ccaagccgat agaatcagcg
     2521 acctttttac cgaccgcgcc ggaacgattt acgccgtcaa cgcgggctgg aacatcaacc
     2581 aatttgacgg gcggcgcttc gtttccgtca gaccgaatct gcccgccgac ctcagcaatt
     2641 tctggattca tcatattacc aaagacagcc gcgacgaatg gtggataccg acgctgaaag
     2701 gtcttttcag atttccgcgc gtcgagcgcg tcgaacaatt agcgacggca aaaccgatcg
     2761 ccgtttatac aatccgcgag ggtctgccga cggacagcgt gctcgtgacg tttgaagatt
     2821 cgcgcggcga tatctggttc agctatgaaa acgcgccggg gcgcatctcg cgctgggaac
     2881 gcgcgaccga aacgtttcat cattacgcgc cgcagaacgg cattcccgaa gcgtgccgac
     2941 cgttttacta taacgaggac gcggcgggcg cgttgataat tacctgccag ttcgaatctt
     3001 atgcgatcta tcgcggcgac gggcgcttta ccgcgcacag cacgccggaa atgaaaaaag
     3061 ccggatggct gcaccgcgcg ctggtcgata gaaaggggcg attctggata gggacgccgc
     3121 tcaacggctt gctgcgttac gacaatccga cttcggaaaa cccgaccgaa accgtctatc
     3181 tgaaaaaaga cggtttgagc agcagccaca ttcaatatct ggtcgaagat ttatacggga
     3241 aaatctatta cgtgacgagc agcggaatgg acagaatgga cacggaaacg ggcgaaatca
     3301 aatattacaa cgtcgcggat ggctttcccg ccgccggaac gggcgtggcg atgcgcgatg
     3361 cgggcggcgc gttgtggctc ggcacctcgc gcggactttc gaaattcgtt cccgaacccg
     3421 acaagccgcc gccgccggtt tttatcagca gcctgcggat tgcgggaaac gacgttccgg
     3481 tttccgtgct gggcgagacg cagatttccg atttaacgct ggcgcctgac gagcgacaga
     3541 tgcagattga tttttacggt ttgagcctcg cttcgggcga aacgcttcgt tatcaataca
     3601 agctcgaagg cgtgagcggc gaatggtcgc cgccgaacga acagcgaacg gtcaattacc
     3661 cgaatctcac ttccggctcg tatcgttttc tggtgcgcgc cgttaatccc gacggcagtt
     3721 tcagcgccga gcccgcttcg atttcattca cggttttgcg tccgctctgg cagcgatggt
     3781 ggtttctgct gctggcggcg ctcgccgtcg gcgcgggcgt ctacgcgttt tatcgttacc
     3841 gcattgccca gattatcaag ctcgagcgcg tccgcacgcg catcgccacc gatttgcacg
     3901 acgacatcgg ctcgtcgctt tcccaaattg cgattttgtc cgaagtcgtc cggcagaaag
     3961 tcggtgcggg cgtcgcgggc gagccgctga atatgattgc cgaaacctcg cgcgagatgg
     4021 tcgacgcgat gagcgacatc gtctgggcaa tcaacccgca gaaagattat ctgaacaatc
     4081 tggtgcagcg aatgcggcgt tttgccgaag acatcctcga cgcgcaggaa atttcttatc
     4141 gattcaattc gctcgaaaat ctgaaggacg tgtccgtcgg ctccgacgtg cggcgcgaag
     4201 cttatatgat tttcaaggaa tgcgtcaaca atgcggtcaa acattccggc gcgaaaaaaa
     4261 tcgattttcg catcaatctg gaaaacgaat ttctggtcat cgaaatcagc gacgacgggc
     4321 gcggcttcga cacggaggaa gcggaaaacc tggacagcct cggcggcaac ggtttaatca
     4381 atatgcggcg gcgcgcagac aatctcggcg gcacgtttct gattgattcc gcgccgggaa
     4441 aaggcacgcg cgccgtcatc agaattccga tcgtcaaaaa attattccgc gttta
//
LOCUS       DASWNE010000466         5807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_16018, whole genome shotgun sequence.
ACCESSION   DASWNE010000466 DASWNE010000000
VERSION     DASWNE010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16018"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            437..1192
                     /locus_tag="VGB68_09260"
     CDS             437..1192
                     /locus_tag="VGB68_09260"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY0049459.1"
                     /translation="MSNTLNSRHLIRNRLLSVLPAGEIDLLRPNLELTSLVPGDVLYQ
                     AGDAIRYVYFPNNGMVSLLAVTEQGQTVEVGYTGYEGMVGLPCILGQGEMAYQAMVQV
                     AADCFRVDARIIQGLFKKNGTFHDVALRYVYVIIKQISQTCVCNHFHTIEARLCRWLT
                     VMCERSNNKQLSLTQEFLSQMLGVQRTSVGMIANSMQASGIIRYSRGKIEIVDFERLK
                     QCACECYFIVRDEYKKFLNDKNFTDMSGNRQTP"
     gene            1262..1654
                     /locus_tag="VGB68_09265"
     CDS             1262..1654
                     /locus_tag="VGB68_09265"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0049460.1"
                     /translation="MQDSNTGTSPKKRILCVEDDRDTCELLSVLFSEYDVVFAGSLRE
                     AFSLLESEHFDLYVLDNWLPDGSGIEVCRKIHELKPDMPIIFTSAVGQKSEIKKALAA
                     GAREYLVKPYEPEELQKVVKELILNHQN"
     gene            complement(1656..2564)
                     /locus_tag="VGB68_09270"
     CDS             complement(1656..2564)
                     /locus_tag="VGB68_09270"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: protein motif:HMM:NF012571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HEY0049461.1"
                     /translation="MLTETISTAVEPAFKISNFEVKDEDLPEFRDMKFEEINQLHIDH
                     PGASDLEYRTRRDYIASLSKQFRETGVITDVEYTQEEQNIWRVVATRLEELQSKRACA
                     FYLEAKKKLDISNERIPQLSEMNRRLKKLTNFRLAPIEGLVETRGFLSWLSYRTMLCT
                     QYIRHASRPEYTPEPDIVHEAIGHIPNFTNPDFADFSQFIGHGARIATDEQLEELGRL
                     YWFTVEFGLIREGGEIKAFGAGLLSSFGELEHAFTSAVERRPFDLKQVINTSYDYSEM
                     QPVLYVISSYAELKGVTRKYIESFGK"
     gene            complement(2848..5031)
                     /locus_tag="VGB68_09275"
     CDS             complement(2848..5031)
                     /locus_tag="VGB68_09275"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY0049462.1"
                     /translation="MKKIQWLVVAIFALASFSSAQDKMLSIDDIFDPQKRVNFSGRPT
                     SVRWSREGATYKQVQNQMLMRVNALTGESREFFDNKKLVSVLTQTTGFSLEEANKLGT
                     TPAQQFNNAETVILINHKNDLYLYNIAGGAIRRLTNTPAAEEMEADFSPDDKMVSFVR
                     GMNLFVVDAATGRETQLTRDGGEKILNGYLDWVYEEELYGRGQKRGYWWSPDSTHIAF
                     LRTDETPVPKFVLPDDTVNDQRVENTDYPQAGDPNPLVRLGAVNVRTRAVRFADTTAY
                     EPKDFLISRVTWSPDSQAMIYQAQNREQTFLDLNSANRATGKTVTVFRETSPAWVEVI
                     DNPVFLKDKSMIWQSARSGFKHLYHYDSAGKMIRRITDGRWEVRSFYGVDERNGWVYF
                     SATEYDPIGEQIYRIKPDGTGLTRLTKTDGSHSANFNPTFTHFVDNWSDVNTPTQTRL
                     FRADGTLEKVLNENKVDALAQYKLGKVELMRVRTRDGFAMEAMMIKPPDFDPSKKYPV
                     MSYTYSGPHSPSVRNAWGGSRYMWHQMLAQKGYIVWICDNRTASGKGAESAWKGYQNL
                     GELELKDLEEGLDALRAMPFVDASRIGLWGWSYGGFMTSYALTHSKSFKMGIAGGTVS
                     DWRLYDSIYTERVMKTPQNNPGGYDKSSVLKAAKDLSGRLLLIHGAMDDNVHMQNTTQ
                     LAYELQKAGKQFDLMIYPTQRHGVVNPLQAKHMYQMMTDYILKNL"
     gene            complement(5088..>5807)
                     /locus_tag="VGB68_09280"
     CDS             complement(5088..>5807)
                     /locus_tag="VGB68_09280"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEY0049463.1"
                     /translation="VFIALSFLFLVSINAQTAAVNQPTVQVTGTAEIQVVPDEVTFSL
                     RVTKSDKSLQLAKTQNDENIARIIALTKRFAIDAKDVKTDFISVSEKFDRVKPKDDDE
                     YQNVFAGYTVSKTVVVKLRDLSKFENFLSEIVKIGITQIGGVSFQSSEIRKHKDRARA
                     MAIRAAKEKAASMTQEIGQGIGKAVSIEEEDVDGDRSLNSNVASNSFSFGLVAESGES
                     ETIAPGTITIRARVEVRFLLY"
BASE COUNT         1409 a         1487 c         1392 g         1519 t
ORIGIN      
        1 gatattaaaa accgttaatt tcccgacttt atcagcgctt ggaaaaattt ctctgacttc
       61 ggaaatagaa gtaaagttat tctgcctcat ttgttgatac cactgtgcta aagcgttttg
      121 cgtttcagga aatttttctg caaactcatt taaacgtttg cgagtgataa cgtgcatatt
      181 caaaaccttt taacaacacg gcgcttcaag ggcaatttct tttttatcta ttaattacaa
      241 aaaattttgc tttatgtcgg atacgcaaca gacaaattgc agcgtttccg ttcagaatga
      301 attcgtcctt caaaacacga gattgtttcc ccaaactctt ttgtgttgcc attgtatttt
      361 tcgggttccg ttccggtaaa taatacaagg cttccccgtt ataaaacgaa aatttggaac
      421 gacgcgcaat gggtgtatgt caaatacatt aaattcacgt catttgattc ggaacaggct
      481 gctttcggtt ttgcctgccg gagagattga cctgctcaga cctaatttgg aattgacctc
      541 actcgtgccc ggcgatgttc tttaccaggc gggcgacgcg attcgctacg tctattttcc
      601 gaataacgga atggtttcgc tgctagccgt gaccgaacag ggacaaaccg tcgaagtcgg
      661 ctacaccggc tatgaaggaa tggtcggtct gccgtgcatt ctcgggcagg gcgaaatggc
      721 gtatcaggcg atggtgcagg tcgccgccga ttgttttcgc gtcgatgccc gcataatcca
      781 gggtttattt aagaaaaacg gaacgtttca cgacgttgcg ctgcgctacg tttacgtcat
      841 tatcaagcag atttcacaaa cctgcgtctg caatcatttt cacacgattg aagcgcgtct
      901 ctgccgctgg ctgaccgtga tgtgcgagcg ctcgaataat aaacaattgt cgctgacgca
      961 ggaatttctc tcgcaaatgc tcggcgtcca gcgcaccagc gtcggaatga tcgccaattc
     1021 gatgcaggcg agcggcatca tccgttatag ccgcggcaaa atcgaaatcg tcgatttcga
     1081 acgcctcaaa caatgcgcct gcgaatgtta ctttatcgtc agggacgaat acaaaaaatt
     1141 tctgaacgac aaaaatttta ccgatatgtc aggtaaccgg cagacgccgt aaagcaatat
     1201 ctttaaaaat gtaatcgttt cgcccggcaa tctgtttcaa tcaacaaaca aagggaattt
     1261 tgtgcaagac tcaaacaccg gcacttcgcc gaagaaaagg attctgtgcg tcgaggacga
     1321 tagagatacg tgtgagcttt taagcgtgtt attttccgaa tacgacgtcg ttttcgccgg
     1381 aagtttacgg gaagccttct ctctgctgga aagtgagcat ttcgatttat acgtgctgga
     1441 caattggctg cccgacggtt caggcatcga agtctgccgt aaaatccacg agctcaagcc
     1501 ggacatgccg ataatcttca cctcggcggt cggacagaaa tccgagataa aaaaagctct
     1561 cgccgccggc gcgagagaat atctggtaaa gccttacgaa ccggaagaac tgcaaaaagt
     1621 tgtcaaagag ctgatactga atcaccaaaa ttgaattact ttccaaaact ttcgatatac
     1681 ttgcgcgtga cgcccttcaa ttcggcgtac gacgaaataa catacaaaac cggctgcatc
     1741 tccgaatagt cgtacgacgt attgatgacc tgttttaaat cgaacgggcg acgttcgacc
     1801 gcgctcgtaa aagcgtgctc cagctcgcca aaactcgaca gcaagccggc gccgaaagcc
     1861 ttgatttcac cgccttcccg aatcaaaccg aattcgaccg taaaccagta gagccgtccg
     1921 agctcttcca gctgttcgtc ggtcgcaatc cgcgcgccgt gaccgataaa ctgcgaaaag
     1981 tccgcaaaat cgggattcgt aaaattcgga atatgcccga tggcttcgtg tacgatgtcg
     2041 ggttcgggcg tgtattccgg acgcgaagcg tggcggatat actgcgtgca gagcatcgtc
     2101 cggtaggaaa gccagcttaa gaaaccgcgc gtttcaacca agccttcaat cggcgcgagc
     2161 cggaaattcg tcagtttttt taagcgtcga ttcatttccg aaagctgcgg aatgcgctcg
     2221 ttgctgatgt ctaatttttt cttcgcttcg agataaaagg cgcaggcgcg tttcgattgg
     2281 agttcttcga gccgcgtggc gacgacgcgc cagatgtttt gttcttcctg cgtgtactcg
     2341 acgtccgtaa tcacgccggt ttcacgaaat tgcttggaga gagaggcgat gtaatcgcgg
     2401 cgcgtgcggt attccaaatc actggcgccc ggatggtcga tatgaagctg attgatttcc
     2461 tcgaatttca tatcgcgaaa ttcgggcaaa tcttcgtctt tcacctcaaa attggaaatt
     2521 ttaaaagcag gttcgacagc ggttgaaatg gtttcggtca gcatattcgg ctcgtctcct
     2581 ttatcaaatt cagatgctca aacaaaaaag aaagatagct gcaaataaac aattgtaatc
     2641 gaaaccatca atcgattaaa tagttaatct atgtttttct ttaaaacaag ctttacattt
     2701 taaggaaaat cgccaaatta ttttatgaat taaatgccgg ctcgggtttc ggagaatatc
     2761 aactaaagaa acgcaatccg caaatgcgaa cgggaagcgg aaaacaatgt gtcgtttccc
     2821 gcttcccgtt cgcatttttc cgtccgatta taagtttttc agaatataat cggtcatcat
     2881 ctgatacata tgtttcgcct ggagcggatt gacgacgccg tgtcgctgcg tcgggtaaat
     2941 cattaaatca aactgtttac cggctttttg cagttcgtag gcaagctgcg tcgtgttttg
     3001 catatgcacg ttgtcgtcca tcgcgccgtg aatcagcagc agcctgccgc tcaagtcttt
     3061 cgcggctttc aaaacggaag atttgtcata tccgcccgga ttgttctgcg gcgttttcat
     3121 cacgcgttcg gtataaatcg aatcatacag acgccagtcc gaaaccgttc cgcccgcgat
     3181 tcccatttta aaagatttgc tgtgcgtcag cgcgtatgag gtcataaaac cgccgtagct
     3241 ccagccccac aaaccaatgc ggctggcatc gacaaacggc atcgcgcgca gcgcgtccaa
     3301 gccttcttcc aaatccttca attccagctc gcccagattc tgatagcctt tccaagccga
     3361 ttccgcgcct ttgccgctcg ccgtgcggtt gtcgcaaatc cacacgatat aacctttctg
     3421 cgccagcatc tgatgccaca tataacgcga gccgccccag gcgtttctga ccgaaggcga
     3481 atgcggaccg gaatacgtgt agctcatcac cggatatttt ttagacgggt caaaatccgg
     3541 cggcttaatc atcatcgctt ccatcgcgaa accgtcgcgc gtgcggacgc gcatcagctc
     3601 gactttgccg agtttatact gcgcgagagc gtcaacctta ttttcgttca aaactttttc
     3661 cagcgttccg tcggctcgga aaagtctggt ctgcgtcggc gtgttgacgt cgctccagtt
     3721 atcgacaaaa tgcgtaaagg tcggattaaa attggcgctg tgcgagccgt cggttttcgt
     3781 caggcgcgtc aagccggttc cgtccggttt aatgcggtaa atctgctcgc cgatcgggtc
     3841 gtattcggtc gcgctgaaat aaacccagcc gtttcgctcg tcaacgccgt aaaacgagcg
     3901 cacttcccaa cgtccgtcgg taatccggcg aatcattttg cccgccgaat cataatgata
     3961 aagatgcttg aagccgctgc gcgccgattg ccaaatcatc gatttgtctt tcagaaaaac
     4021 gggattatca atcacttcga cccacgcggg cgaagtttcg cggaaaacag tgacggtttt
     4081 tcccgtcgcg cgattcgcgc tgtttaaatc aagaaacgtc tgctcgcggt tctgcgcctg
     4141 ataaatcatc gcctggctgt cgggcgacca ggtcacgcgc gaaatcagaa aatctttcgg
     4201 ctcgtaggct gtcgtgtcgg caaagcggac ggcgcgcgtt ctgacgttga ccgcgccgag
     4261 cctgaccagc ggattcgggt cgcccgcctg cgggtaatcg gtgttttcga ctcgctggtc
     4321 gttaaccgta tcgtccggca agacgaattt cggaacgggc gtttcgtcgg ttctcagaaa
     4381 agcgatgtgc gtcgaatcgg gcgaccacca atagccgcgt ttctgcccgc gcccgtagag
     4441 ttcttcctcg taaacccagt cgagatagcc gttcagaatt ttttcgccgc cgtcgcgcgt
     4501 cagctgcgtt tctctgccgg tcgccgcgtc gacgacgaat aaattcattc cgcgtacaaa
     4561 gctgaccatt ttatcgtcgg gcgaaaaatc ggcttccatt tcttccgccg ccggcgtgtt
     4621 cgtcaaacgc cgaatcgcgc cgcccgcgat gttgtaaaga tacaaatcgt ttttgtgatt
     4681 gattaaaatc acggtttccg cgttgttgaa ctgctgcgcg ggcgtcgttc ccagcttgtt
     4741 cgcctcttcg aggctgaatc cggtcgtctg cgtcagaacg gaaacgagtt ttttattatc
     4801 gaaaaactca cgcgattcgc ccgtcagggc gttgacgcgc atcagcatct ggttctgcac
     4861 ctgtttatac gtcgcgcctt cacgactcca gcgcacagac gtcggtctgc cgctgaaatt
     4921 gacgcgcttt tgcgggtcga aaatatcgtc aatcgaaagc attttgtcct gcgccgagga
     4981 gaaagaggcg agcgcaaaaa ttgcgaccac aagccactga atttttttca tagttttttc
     5041 aagttttcag gaaagatatt gtttgatagc agtataaacg aatccgtcta atacaacaaa
     5101 aatctaacct cgacccgcgc ccggatggta attgtgccgg gcgcaattgt ttccgattca
     5161 ccggattcgg ctactaaacc gaaagaaaac gagtttgacg caacatttga attcaagctg
     5221 cggtcgccgt cgacgtcttc ctcttcaatc gagacggctt tcccgatgcc ttgtccgatt
     5281 tcctgtgtca tcgacgcggc tttttccttt gccgcgcgga ttgccatcgc tcgcgcccgg
     5341 tctttatgct tgcgaatttc cgaagattga aagctgacgc cgccgatttg cgtgatgccg
     5401 attttgacaa tctcggataa aaaattttca aacttgctca aatctcttaa tttcacgacg
     5461 acggttttgg aaaccgtata accggcgaaa acgttttgat attcgtcgtc gtctttcggt
     5521 ttaacgcggt cgaatttttc gctgaccgag atgaaatccg tcttgacgtc tttcgcgtca
     5581 atcgcaaagc gttttgtcaa agcgattatc cgggcgatat tttcgtcatt ctgcgttttt
     5641 gcgagctgca ggctcttatc gcttttcgtc acgcgcagcg agaatgtaac ctcgtccggc
     5701 acaacctgaa tttccgccgt tccggtcacc tgaaccgtcg gctgattaac cgccgccgtt
     5761 tgagcgttga ttgaaacgag aaacagaaag cttaaagcaa taaaaac
//
LOCUS       DASWNE010000467         1816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_156936, whole genome shotgun sequence.
ACCESSION   DASWNE010000467 DASWNE010000000
VERSION     DASWNE010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156936"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..1440)
                     /gene="rho"
                     /locus_tag="VGB68_09285"
     CDS             complement(193..1440)
                     /gene="rho"
                     /locus_tag="VGB68_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEY0049464.1"
                     /translation="MNLAVLKEMSISQLTTIAKDLGVEGASGMRKQELIFKVLAAQTE
                     KSGLIFSEGVLETLPDGFGFLRAPEYNYLPGPDDIYVSPSQIRKFDLRTGDTVSGQIR
                     PPKEGERYFALIKVEAINFEAPEHSREKIFFDNLTPLYPDDQLKMEVTSDQIAARVID
                     LVTPIGKGQRALIVAPPRTGKTMLLQTIANSITENHPEVTLIVLLIDERPEEVTDMQR
                     SVKGEVISSTFDEPPTRHVQVADMVIEKAKRLVEHKRDVVILLDSITRLARAHNAVVP
                     PSGKILSGGIDSNALQRPKRFFGAARNIEEGGSLTIIATALIDTGSRMDDVIFEEFKG
                     TGNSEIHLDRRLSDKRLFPAIDLQRSGTRKEELLINKEDLNRIWVMRRVLNPLSPVEQ
                     MEVVLERLSKTKTNAEFLASMQT"
BASE COUNT          386 a          472 c          476 g          482 t
ORIGIN      
        1 acgtcttccg gcgaaaacgt tccggcaaag tcgtagaaca gcttcgcctt aatatccgac
       61 actttatacg aatacggctt ttgcgccgac gccgaaacac atattaaagc cgataaaacg
      121 aaagcgaaaa acagttttct catttttcaa aaagaaaaga ggaagcagtt tattacttcc
      181 tctctaatta aattaggtct gcatcgaggc gagaaattcg gcgttcgtct tggtcttcga
      241 caagcgttcg agcacgactt ccatctgttc gacgggcgag agcggattca ggacgcggcg
      301 cataacccag atgcggttga ggtcttcctt gttgataagc aattcctctt tgcgcgttcc
      361 cgaacgctgt aaatcgattg ccggaaacag gcgcttgtcg gacagacgcc ggtcgagatg
      421 aatttccgag ttgccggttc ccttgaattc ttcgaagata acgtcgtcca ttctggagcc
      481 ggtatcaatc aaagccgtcg cgataatcgt caggcttccg ccttcttcga tgttgcgcgc
      541 cgcgccgaaa aatcgtttcg ggcgctgaag cgcgttcgag tcgataccgc cggaaagaat
      601 cttgcccgac ggcggaacga ccgcgttgtg cgcgcgggcg agacgcgtaa tcgagtcgag
      661 caggataacg acgtcgcgtt tgtgctcgac caggcgtttc gctttttcga tgaccatatc
      721 ggcgacctgc acgtggcgcg tcggcggctc gtcgaaggtc gacgaaatga cttcgccttt
      781 gaccgagcgc tgcatatcgg tgacttcttc cggtcgctcg tcaatcagca gaacgattag
      841 cgtcacttcc gggtgatttt ccgtgatcga attggcgatg gtctgcaaaa gcatcgtctt
      901 accggtgcgc ggcggcgcga caatcaatgc gcgctgacct ttgccgatgg gcgtcaccag
      961 atcgatgacg cgcgccgcga tctggtcgga cgtgacttcc attttcagct ggtcgtccgg
     1021 gtagagcggc gtcagattgt caaaaaatat tttctcgcgc gaatgttccg gcgcttcaaa
     1081 gttgattgct tcgaccttga taagcgcgaa ataccgctcg ccttctttcg gcgggcgaat
     1141 ctgtccggag acggtgtcgc cggtgcgcaa atcgaatttg cgaatctgcg aagggctgac
     1201 gtaaatatcg tccggtcccg gcagatagtt atattccggc gcgcgcagaa agccgaaacc
     1261 gtccggcagc gtctccagaa cgccttccga gaatatcaga ccgctttttt cggtctgcgc
     1321 ggcgaggact ttgaaaatca actcctgctt gcgcataccc gatgcgcctt cgacgcctaa
     1381 atccttagcg atggtggtca attgcgagat tgacatctct ttcagaaccg ccagattcaa
     1441 cagttcgccg tcgttattat aagtgtccgc tttttcatcc cggcggttgt tcccgtccgt
     1501 cttggtgctg ccgtccgttt cagtattagt ctcggtttgc gcgtccatcg tcgtttgttc
     1561 attgtcctgc gcatctttta gcgcaagccc gttttcttcc gtttttgcag ctcttactgt
     1621 gcggctgcgt gttgttttcg ttgccatgtt ttatacccgt atgtctattg ttagaaaaga
     1681 actgtcgaat gtgaagaatg ccaccctcaa ctgtcgagtt ttacaaaaaa ttatcatcct
     1741 cgtagtagat tgatttgctt aactcttgga gatatttatg gaaagttcag taggggcgta
     1801 aacttgattc aggctc
//
LOCUS       DASWNE010000468         3501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_441243, whole genome shotgun sequence.
ACCESSION   DASWNE010000468 DASWNE010000000
VERSION     DASWNE010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441243"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            251..1780
                     /locus_tag="VGB68_09290"
     CDS             251..1780
                     /locus_tag="VGB68_09290"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0049465.1"
                     /translation="MIKSDEGKTVSYWEASEDLLNKSALGDSVETDVCIIGGGISGLT
                     TAYLLTKAGKKVVVIDDGAIGGGETSRTTAHLSNALDDRIYRIEKWHGEEKARLAVQS
                     HGAAIGEIERIIETEGIECDFRRVDGYLIDAGDESEDDLNEELEAAHRVGFAEVERVG
                     RAPIKDFDTGECLKFPRQGQFHILKYLAGLAKAVEQNGGRLFSGTRAVEWKGADSPQV
                     KTSNNQTIRARSIVLATNYPIMSKMFAKLPAYRTYVIGARVPKDSLEKALFWDTADPY
                     RYVRTQPENDYDVLIVGGEDHRTGQEDDGDERFARLWDWTRERFPMAEEILYRWSGQF
                     METHDGLAFIGTFSGSEPNVYLITGDSGMGMTHGTIGGMLVSDLILGRENPWREVYEP
                     SRLATQSIMEAVPEIISSTAPYVDWLTGGDVSSVEEIKPGEGAIISHGTTKIAAYRDE
                     TGKLYERSAVCAHLGCIVRFNSLEKTWDCPCHGSRYGIDGHPINAPAFKSLAEIEEES
                     S"
     gene            1910..>3501
                     /locus_tag="VGB68_09295"
     CDS             1910..>3501
                     /locus_tag="VGB68_09295"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY0049466.1"
                     /translation="MKKVLFFCFVLLFLSQTSNAQETFFQTGHTNDILEVHFSPDDSQ
                     LISYSGGDGRFILWDVKSGRQLWMSETSFIRKGDESINLKEFYWSKDGKFLVTKSING
                     TYQTWDANAGKILALTGQKPEIELITPDKKQISYARDYDYIIVRQAETGEIIKIKRFG
                     NNSAFNTSNEATMIAEGGGWGDASIRITEIKTGKFRWLDGHPSVVGEIVFSPDGKFLA
                     VGGSDRIIYIFDAASKTLAKKISGNTKPIVSLAFSPDGKILVSAEDNGILRVWNWQDA
                     ALLREVKSEEDILGAEKVSFSADGKYLLTSSDRTEFRLWDAKSFKLLRNFKTAEKYES
                     TAGMLTLGYDSVPVSSVNFSRDGRRIISAHAGDGTVRIWNPDSGSQIKKLKVCQSVSF
                     ASFTNDERKILAYCDKRDEEKIKIFDAETGREIFRFDDAETGFIKTVSLSADGRHFAT
                     SDVGGDVLLWDINKSKPIREPDIGFSGDDAIAFSPDGKTFAVGGRNQNLFLFDVETGE
                     KLWQLIASYQPGELEKKLEEEK"
BASE COUNT          957 a          824 c          960 g          760 t
ORIGIN      
        1 gccttcgcca gataacacgc gcacgtcaag ccgttgtgcc cgccgccgat aacggcgacg
       61 tcgtaagtct tattcataaa aagattttgc cacagaagag atttagccgc gaaactaccc
      121 aaaagaagag gaaataaaat caaattattt tttttgtgcg gctgaatttt tttatcaaac
      181 tcgcagaaaa agttttataa tcaattacac gcagcattaa attaaatttt gaacaaaggg
      241 aagcagaaaa atgataaaaa gtgatgaagg gaaaaccgtt tcatactggg aagcgagcga
      301 ggatttgctg aataaaagcg cgctgggcga ctcggtcgaa acggatgttt gcattatcgg
      361 cggcggaatt tccggtttga cgacggctta tcttttgacg aaagccggaa aaaaggttgt
      421 ggttatcgac gacggcgcaa tcggcggcgg cgaaacctcg cggacgacgg cgcatctttc
      481 caacgcgctg gacgaccgca tttatcgcat cgaaaaatgg cacggcgagg aaaaagcgcg
      541 tcttgcggtg cagagtcacg gcgcggcgat cggcgaaatc gaaagaatca tcgagaccga
      601 aggaatcgag tgcgattttc ggcgcgttga cggttattta atcgacgccg gcgacgagag
      661 cgaggacgat ttgaacgagg aactggaagc cgcgcatcgc gtgggattcg cggaagtcga
      721 acgcgtcggg cgcgcgccga ttaaagattt cgatacgggc gaatgtctga aatttccgcg
      781 tcaggggcag tttcacattc ttaaatatct cgccggttta gccaaagccg tcgagcaaaa
      841 cggcggaagg cttttttccg gcactcgcgc cgtcgaatgg aaaggcgcag attctccgca
      901 ggtgaaaacc tcaaacaacc agacgattcg cgcccgctca atcgtgctcg ccacgaatta
      961 cccgataatg agcaaaatgt ttgccaagct tccggcgtac cggacatacg ttatcggcgc
     1021 gcgcgttccg aaagattcgc tggaaaaagc tttgttttgg gacacggctg acccgtatcg
     1081 ctacgtccgc acgcagccgg aaaacgatta cgacgttttg attgtcggcg gcgaagacca
     1141 tcgcaccggg caggaagacg acggcgacga gcgtttcgcg cggctgtggg attggacgcg
     1201 ggagcgtttt ccgatggcgg aagaaatttt atacagatgg tcggggcagt ttatggaaac
     1261 gcacgacggg ctggctttta tcggcacatt ttccggctcg gagccgaatg tttatctgat
     1321 aacgggcgat tcgggaatgg gcatgacgca cgggacaatc ggcggaatgc tggtttcgga
     1381 tttgattctg ggacgcgaaa atccgtggcg ggaagtttac gagccttcgc gtctggcgac
     1441 gcagtcaatc atggaagccg tgccggaaat tatcagctcc accgcgccct atgtcgattg
     1501 gctgacgggc ggcgacgttt cctcggtcga agaaatcaaa ccgggcgaag gcgcaatcat
     1561 cagtcacggc acgacgaaaa tcgccgctta ccgcgacgaa accggaaagc tttacgagcg
     1621 ttccgccgtc tgcgcgcatc tcggctgcat cgtgcgtttc aacagcctgg aaaaaacctg
     1681 ggattgcccg tgtcacggct cgcgctacgg catcgacggg catccgataa acgctccggc
     1741 ttttaagagt ctggcggaaa tcgaagaaga atcttcgtaa gttttcggaa aaggcgcggg
     1801 cttaaaatcc gcgccttaaa ctttcctctc ggcgcgcgga acttttttat ttttcaggca
     1861 tctaaccgga taacgcggcg gtttaataaa taattaggtg tcaaccttta tgaaaaaagt
     1921 tctctttttc tgtttcgtcc ttttatttct ttcgcagacg tcaaacgcgc aggaaacttt
     1981 ttttcaaacc ggacacacga atgacattct cgaagttcat ttcagtcccg acgacagcca
     2041 gctaatttcg tattcgggcg gcgacgggcg ttttatcctc tgggacgtaa aaagcggtcg
     2101 gcagctttgg atgtcggaga cgagctttat tcgcaaaggt gacgaaagca ttaatctgaa
     2161 agagttttac tggagcaaag acggaaaatt tctcgtcacg aaaagcatca acggaactta
     2221 tcaaacgtgg gacgcaaacg ccggaaaaat ccttgcttta accggacaaa aacccgaaat
     2281 cgaattaatc acgccggata aaaaacagat ttcctacgcg agagattacg attatataat
     2341 cgtgcgccaa gccgaaacgg gcgaaattat aaaaatcaaa aggttcggaa acaattccgc
     2401 ttttaatacg agcaacgagg cgacgatgat tgccgaaggc ggaggatggg gcgacgcttc
     2461 aatcagaatt accgagatta aaaccggaaa attccggtgg ctcgacggtc atccgagcgt
     2521 cgtcggcgaa atcgttttca gtccggacgg gaaattttta gcggtcggcg gcagcgacag
     2581 aattatttac attttcgacg cggcgagcaa aacgctggcg aaaaagattt ccggaaacac
     2641 caagccgata gtttctctgg ctttcagccc ggacggaaaa attctggttt cagccgaaga
     2701 caacggaatt ttaagagtct ggaactggca ggacgccgcg ctgctgcggg aagtaaaatc
     2761 ggaagaagat attttgggcg cggaaaaagt ttcgttcagc gcggacggaa aatatctgct
     2821 gaccagcagc gacagaaccg agttcagact ctgggacgcg aaaagtttta agcttttacg
     2881 taactttaaa actgcggaaa aatatgagtc cacagccgga atgctgacac tcggctatga
     2941 ctccgtgccg gtttcttcgg taaatttcag ccgcgacggg cggcgcatta tttccgctca
     3001 cgccggcgac gggacggtga gaatctggaa tcccgacagc ggcagccaaa taaaaaagct
     3061 caaagtctgt caaagcgtgt ctttcgcttc ttttacaaac gacgaacgaa aaattctcgc
     3121 ttactgcgat aagagagacg aagaaaaaat caaaattttc gacgccgaaa cggggcgcga
     3181 gattttcaga ttcgacgacg cagaaaccgg ctttattaaa accgtctcgt taagcgcgga
     3241 cggcaggcat ttcgcgactt ccgacgtcgg cggcgacgtt ctgctctggg acattaacaa
     3301 atcgaaaccg attcgcgagc cggacatcgg attttccggc gacgacgcga ttgccttcag
     3361 cccggacgga aaaactttcg ccgtcggcgg gagaaatcag aatttgtttt tgttcgacgt
     3421 ggaaacgggc gaaaagctct ggcagttgat agcttcgtat cagccgggcg agctggaaaa
     3481 aaaactggaa gaagaaaaag a
//
LOCUS       DASWNE010000469         3324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_612336, whole genome shotgun sequence.
ACCESSION   DASWNE010000469 DASWNE010000000
VERSION     DASWNE010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612336"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VGB68_09300"
     CDS             <1..350
                     /locus_tag="VGB68_09300"
                     /inference="COORDINATES: protein motif:HMM:NF019982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0049467.1"
                     /translation="IPAFTPEFAAARADVIEKVCRLRTENVVPEKVYTRQMTSAVTFD
                     AEKRVSRIQAETLVLTGDRDIVVPPQNSFNLARAIPKASLKIIENGSHMFFIENAAEF
                     NRIVTNFLKDGSV"
     gene            451..684
                     /locus_tag="VGB68_09305"
     CDS             451..684
                     /locus_tag="VGB68_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049468.1"
                     /translation="MYEKGFCRFYKSEIPATPSDWKHRICKNIQLNIFFEWHLRQISL
                     AERLGWFGQNLEENVLYEFPYNRPDKIKKLKDL"
     gene            691..1683
                     /locus_tag="VGB68_09310"
     CDS             691..1683
                     /locus_tag="VGB68_09310"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEY0049469.1"
                     /translation="MRNGKIIGSGVHVPEKIITNDDLSRMLGEDINEFVSQNLGIQQR
                     HVLAENESAADLAAAAAANALKDAGISAEEIDLIVVSTDTPEYISPSTSVVVQHRLGA
                     KNAGTFDTNAACAGFVTAFDAALKYIIADKNYKNVLVIGVYAMSKFLDWTEKKTATIF
                     ADGAGAVVLQAIEEENAGWLASKLEAKGEYHDFLGIFAGGSKYPINAEELQNGYRNKV
                     RFAKRTPPEINFEGWQRIVKEILERAELALDDVDLFLWTQVNLSTIKQVMAALNQPFE
                     KTHTIMDKWGYTGSACIPMVLDDAIRAGKLKRGDTLVFCASGGGINMAAMVFRY"
     gene            1708..>3324
                     /locus_tag="VGB68_09315"
     CDS             1708..>3324
                     /locus_tag="VGB68_09315"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEY0049470.1"
                     /translation="MKFPNIYTLFRAQAAKYRGKPVFYSRENDGWKSRNWEDFEEQVH
                     DFACALLAKGLSKGASVAILAGNVPEWTIVDIATMAAGGVGVGIYPTSSAEQCAYIIN
                     HSDAEFVFVDTPEQLLKILKISDRLPKVRQIIFYAVGKLYKFVEGLPDDEKKLFDDLH
                     KSKIEFYHDFRMFGKVRKDELLPEIEEIGFNLKPEETAIMVYTSGTTGQPKGAMLSHR
                     YILNSCESLRRSVPIYDADVSFSYLPGCHVAERISGIYNRLYNGASVYFVDDLARLYT
                     YMLDVSPTVFASLPRFFEKVYARLVSEVQNLPAREREEFEKSLNVGRGIVRLQMAKQE
                     ISTDLKERFADVSPNFQRVRDAFGGNIRLATSGGAPLPDEIAEFFAAAGLPILQAYGL
                     TENICVAFNRADDFKFGTVGKPMPMCRVKIAADGEILVKSPMMFSGYYKEPEKTAEMF
                     DAEGWLKTGDLGSLDEDGFLKITGRKKEIIVLSTGKNVAPAMLENLLKENHLISQAFI
                     YGDGRSYLTAIITLNRAEAEAHARAASIAFS"
BASE COUNT          861 a          779 c          904 g          780 t
ORIGIN      
        1 tgattccggc tttcacgccc gaatttgccg ccgcgcgcgc cgatgtaatc gaaaaagtct
       61 gtcgcctgcg cactgaaaac gtcgtgccgg aaaaagttta tacgcggcag atgacttccg
      121 ccgtcacgtt tgacgccgaa aagcgcgtct cgcggattca agcggaaact ttagttctga
      181 ccggcgaccg cgacatcgtc gtgccgccgc aaaattcgtt taatctggca cgggcgattc
      241 cgaaggcgag tttgaaaatt atcgaaaacg gaagtcatat gttttttatc gaaaatgcgg
      301 cggagtttaa ccggatagtt acgaactttt tgaaagacgg aagcgtgtaa ttctctgaaa
      361 gacggagaaa aatttatgcc gaatggaaaa ggtaagattg attgttgtta ctgcgttcat
      421 tttcaaagcg agtttgaggg cgcggacgca atgtatgaaa agggattttg ccgattttac
      481 aaaagcgaga tacctgcgac tccatctgat tggaagcacc ggatttgtaa gaatattcag
      541 cttaatatat tttttgaatg gcatttacgg caaatttcgc tggcggaaag gctcggttgg
      601 tttggacaaa atttagaaga aaacgtcctc tatgagtttc cttataatcg tccggacaaa
      661 atcaaaaaat tgaaagattt gtaagagttt atgcgaaacg gaaaaattat cggcagcggc
      721 gtacacgttc ccgaaaaaat tatcaccaac gacgatttga gccggatgct cggcgaagat
      781 attaatgaat ttgtctcgca aaatctcgga attcagcagc gacacgttct ggcggaaaat
      841 gaaagcgcgg ctgatttggc ggcggcggcg gcggcgaacg ctctgaaaga cgccggaatt
      901 tcagccgaag aaattgattt aatcgtcgtc tcgaccgaca cgcccgaata catctcgcct
      961 tcgacatcgg tcgtcgttca gcaccggctc ggcgcgaaaa acgccggaac tttcgatacg
     1021 aacgccgcct gcgccggttt cgtgacggct ttcgacgcgg ctttgaaata cattatcgcc
     1081 gacaaaaatt acaaaaacgt cctcgtcatc ggcgtctacg cgatgtcgaa atttctggac
     1141 tggacggaaa agaaaaccgc gacgattttt gccgacggcg cgggcgcggt cgtgctacag
     1201 gcgatagaag aagaaaacgc cggctggctc gcatcgaaac ttgaagcgaa aggcgaatat
     1261 cacgattttc tcggtatttt tgcgggcggc tcgaaatatc cgattaacgc cgaagagctg
     1321 caaaacggct atcgcaacaa ggtgcgcttt gcgaaaagaa cgccgccgga aatcaatttc
     1381 gaaggctggc agcgcatcgt caaggaaatt ctggaacgcg cggaactcgc gctcgacgac
     1441 gtcgatctgt ttctctggac gcaggtcaat ctttcgacca tcaaacaggt gatggcggcg
     1501 ctcaatcagc cgtttgaaaa aacgcatacg attatggaca aatggggcta taccggctcg
     1561 gcttgcatcc cgatggttct ggacgacgcg attcgcgccg gaaagttaaa acgcggcgac
     1621 acgttggttt tctgcgcttc gggcggcggc atcaatatgg cggcgatggt ttttcgatat
     1681 taattttcca gtgaatttaa aagggaaatg aagtttccaa acatttacac attatttcgc
     1741 gcgcaagccg ccaaatatcg cggcaagccc gttttctatt cacgtgaaaa cgacggctgg
     1801 aaatcgcgaa actgggaaga ttttgaagag caggttcacg atttcgcctg cgctcttttg
     1861 gcgaaagggt tgagcaaagg cgcgagcgtg gcgattttag cgggaaatgt tcccgaatgg
     1921 acgattgtcg atatcgcgac gatggcggcg ggcggcgtcg gcgtcggcat ttacccgacc
     1981 agttcggcgg aacaatgcgc atatattatc aatcattcgg acgccgagtt tgtttttgtc
     2041 gatacgccgg aacagcttct gaaaatttta aaaatttccg accggcttcc gaaagtcagg
     2101 cagattattt tttacgccgt cggaaaactt tataaattcg tcgaagggct tccggacgat
     2161 gaaaaaaaac tattcgatga tcttcacaag tcgaaaatcg agttttatca cgatttccgt
     2221 atgttcggaa aagtcagaaa agacgagctg cttccggaaa tagaagaaat cggcttcaat
     2281 ctgaaaccgg aagagacggc gataatggtc tacacttccg ggacgaccgg gcagccgaaa
     2341 ggcgcgatgc tgtcgcatcg atatatttta aattcgtgcg aatcgctgcg ccgctcggtt
     2401 ccgatttacg acgcggacgt ttcgttttcg tatttgccgg gctgccacgt cgccgagcgg
     2461 atttcgggca tttacaaccg gctttacaac ggcgcgagcg tttattttgt cgatgattta
     2521 gccaggcttt acacgtatat gctcgacgtt tcgccgacgg ttttcgccag cttgccgcga
     2581 tttttcgaga aggtttacgc gcgcctcgtt tccgaagtgc aaaatctgcc ggcgcgcgaa
     2641 cgcgaagaat ttgaaaaatc tctgaacgtc gggcgcggaa tcgtgcgcct gcaaatggcg
     2701 aaacaggaaa tttcgacgga tttgaaggaa agattcgccg acgtttcgcc gaatttccag
     2761 cgcgtgcgcg acgcattcgg cggaaatatc cgtctggcga cgagcggcgg cgcgcctttg
     2821 ccggatgaaa tcgcggaatt tttcgccgcc gccgggctgc cgattctgca ggcttacggc
     2881 ttgaccgaaa acatctgcgt cgcctttaat cgcgccgacg attttaaatt cggaacggtc
     2941 ggcaaaccga tgccgatgtg ccgtgtgaaa atcgctgcgg acggcgagat tctggtgaaa
     3001 agtccgatga tgttttccgg ttattacaag gagccggaaa aaacggcgga aatgtttgac
     3061 gccgaaggct ggctgaaaac cggagatttg ggcagtctgg acgaggacgg ttttttgaaa
     3121 attacggggc gcaaaaaaga aatcatcgtg ctttcgaccg gcaaaaacgt cgcgcccgca
     3181 atgctcgaaa atcttttgaa agaaaatcat ttaatcagcc aggcgtttat ttacggcgac
     3241 gggcgcagct atctgacggc gattatcacg ctcaatcgag ccgaagccga agcccacgcg
     3301 cgcgccgcga gcatcgcgtt ttcg
//
LOCUS       DASWNE010000470         5680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_581115, whole genome shotgun sequence.
ACCESSION   DASWNE010000470 DASWNE010000000
VERSION     DASWNE010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581115"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..1324
                     /locus_tag="VGB68_09320"
     CDS             125..1324
                     /locus_tag="VGB68_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049471.1"
                     /translation="MKGNFDAPEEIGRWRTVALGIGGIAAIVILIAALIMPEMREQAL
                     RSWLLGFCLWGGIGIGSIGILLLQYLTGGAWGVVIRRILEASSRTLPIVFLLFQPILI
                     GDGSLYGWTHPKPGDKIMEIRDAYLNYPFWALRAVLYFLLWGVMAVLLNRWSKQVDDT
                     GDWSLLQKASRFSGPTMVFYVLLVSFAAIDWTMSLDQHWYSTIWGLLFVVGWALAAFA
                     FVIVILAWLSTRAPMNRVVGKRHFHDLGKLVLALVMVWAYFNFSQFLIIWSGNLPEET
                     QWYITRMSGGWGVIGVALIVLHFAFPYLVLLSRDIKRNAKYLAMLAIFILFMRLVDLY
                     YIIGPAPTTTSHAEMGFHISWMDFLAPVAVGGIWLWWFFGELRKRPLVPINDPFLENA
                     IEHGKGH"
     gene            1414..1968
                     /locus_tag="VGB68_09325"
     CDS             1414..1968
                     /locus_tag="VGB68_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049472.1"
                     /translation="MGSTKHEKSFVDYDKSYEQNIVGLRGVVYFGVFLLLLIVVTFVL
                     MFFLINVMEDQASQDKDVMTPMRREAIEKNPDVFLPPEPRLQSAPGFGVDGENGRINL
                     ELKEPQSEWWELERKWNAELANGQKDQKTGTVITLPIEEAKNRLMQENIKARPAEQGQ
                     KALEESRSIISYSSAGRVANEKRR"
     gene            2098..3006
                     /locus_tag="VGB68_09330"
     CDS             2098..3006
                     /locus_tag="VGB68_09330"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY0049473.1"
                     /translation="MKKSSAILAVLRLKLLVSFCIAAFAAFAYGQGQHYNSPLYSPRT
                     YDPAQGTTSNGLPKTLQEVGIEQRLNEPLPLDTEFKDENGKIVKLGEYFGKQRPVILA
                     LVYYECPMLCNEVLNGLTGSLKGNSLDAGKDFDVVAISFDAREYDKPDLAKNKKAGYM
                     RRYDRAGTENGWHFLTGTQESIDSVTQAVGFSYEWDERSQQFAHMGGIMIATPEGKLA
                     RYFYGIDYAPLDLKLGLMDSAQNKIGTKVDQLMLYCYHYDPSTGSYGFAILRAMRLAA
                     VLTLLGIGSMLFVFWRRNKKKVLVNG"
     gene            3061..4215
                     /gene="coxB"
                     /locus_tag="VGB68_09335"
     CDS             3061..4215
                     /gene="coxB"
                     /locus_tag="VGB68_09335"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY0049474.1"
                     /translation="MQQENNYIPLFPEQASTFAWQVDALYVYLILISVFFTVLIVAGI
                     FYFMVRYREREKFENGAEIHGSMMLETTWSIIPFVISMTIFLGGAIVYYNQYRPPKDA
                     MEIYVVGKQWMWKIQHATGQREINELHVPVGHKIKLTMTTEDVLHDFSIPAFRTKMDV
                     VPGRYTTLWFEPTKPGKYHLFCAEYCGLNHSGMGGWVYVMEQREFDNWLSGNTSNQTP
                     VEAGKELFEAKLGCASCHAGGPNQRGAKVEGIFGTQIKLVGGQTVLADEAYIRESILN
                     PGAQIVEGYQPIMPTFKGQVSEEQLVALVAYIKSLSGNNAGSGNTSTTTTTSTSTTTN
                     SNGASRNEVMSPASRRTDTVAPITDRGQSNTQGSNPNAPRNRSEPTNQPR"
     gene            4254..>5680
                     /locus_tag="VGB68_09340"
     CDS             4254..>5680
                     /locus_tag="VGB68_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY0049475.1"
                     /translation="MENVEAMNVGTIRTTPEKNYLNNGYTIASWLLTKDHKRIAIMYL
                     ITVSMFFMAGGLYASAIRLELLTPPSDLFESGTYNKVFTQHGIIMVFFFLIPAIPAIL
                     GNFLIPIMIGAKDLAFPRINLLSLYFYWLGGLLTIWALLQGGVDTGWTFYTPYSTTFS
                     NTYVMAVGLGIFINGFSSILTGLNFIVTTHTMRAPGMTWFRLPLFVWAHYATSLVMLL
                     GTPVIAITILLLALERLTRVGIFDPAIGGDPILFQHLFWFYSHPAVYIMILPGMGIIS
                     ELISNFSRKKIFGYEFIAFSSIAIAVFGFLVWGHHMFASGQSIYAGLVFSFLTMVVAV
                     PSAIKVFNWAATLYKGHISYDTPMLYALFFIGLFLIGGLTGLFLGAMGLDVHLTDTYF
                     VVAHFHYVMVGGQVLAYLGGIHYWWSKMTGRMYSEFWAKIAAMIVFVGFNLTFFPQFI
                     LGYLGMPRRYASYPPEMQVLNIFSTA"
BASE COUNT         1278 a         1389 c         1575 g         1438 t
ORIGIN      
        1 gctgccgacg acttcgccga cgaccggagg cacaaccgcg ccgccgctga cgccgtccgc
       61 tccgtcgacg cccgcgacga acaatgccaa tacgaacacg accaatcagg gaggaaatcg
      121 ttaaatgaaa gggaattttg atgctcccga agaaatcggt cgttggcgaa cagtcgcgct
      181 cggcatcggc ggaattgccg ccatcgtgat tttaattgcg gctttaatta tgcctgagat
      241 gcgcgaacag gcgctgcgct cgtggctgct gggtttttgt ttgtggggcg gcatcggcat
      301 cggctccatc ggtattctgc ttttgcagta tctgacgggc ggcgcgtggg gcgtcgtgat
      361 tcgccggatt ctcgaagcct cttcgcggac gctgccgata gtttttcttt tgtttcagcc
      421 gattttgatt ggcgacggct cgctttacgg ctggacgcat ccgaagcccg gcgacaaaat
      481 tatggaaatc cgcgacgcgt atctgaatta ccctttctgg gctctgcgcg ccgttctgta
      541 tttccttttg tggggcgtga tggcggttct gctcaacagg tggtcgaaac aggtggacga
      601 caccggcgac tggtcgcttt tgcagaaagc ttcgcgcttt agcggtccga cgatggtttt
      661 ttatgttctg ctggtcagct ttgcggcgat tgactggacg atgtcgctcg accagcactg
      721 gtattcgacg atttggggct tgcttttcgt cgtcggctgg gctttggctg cttttgcttt
      781 tgtaattgtg attctggctt ggctttcgac gcgcgcgccg atgaatcgcg tcgtcggcaa
      841 gcgccatttc cacgacctcg gcaaattggt gctggcgctg gtgatggtct gggcatattt
      901 taatttctcg cagtttttga ttatctggtc gggaaatctg ccggaagaga cgcagtggta
      961 tatcacgcgt atgtcgggcg gctggggcgt tatcggcgtc gctttgattg tgctgcattt
     1021 cgcgtttccg tatctggttt tgctgtcgcg cgacatcaag cgcaacgcga aatatttagc
     1081 tatgctggcg atttttattc tgtttatgcg tctggttgat ttgtattaca tcatcggtcc
     1141 cgctccgacg acgacttcgc acgccgagat gggatttcat atcagctgga tggattttct
     1201 ggcgccggtc gcggtcggcg gaatctggtt atggtggttt ttcggcgagc ttcgaaaacg
     1261 tccgctcgtt ccgattaacg acccgttttt ggaaaacgcg attgagcacg gaaaaggaca
     1321 ttgataatta cgaattaaaa attacgaatt acgaatggaa gaatattcaa cggcgtaatt
     1381 cgtaatttga aattcgtaat tcgtgattga attatgggaa gcacaaaaca cgaaaaatcg
     1441 tttgtggatt acgacaaatc ctacgaacaa aatattgtcg ggctgcgcgg agtggtttat
     1501 ttcggggttt tcctgttact tcttatcgtc gtgactttcg tcctgatgtt tttcctgatt
     1561 aacgtgatgg aagaccaggc gagccaggac aaagacgtga tgactccgat gcgccgtgaa
     1621 gcgatagaga aaaacccgga cgtttttctg ccgcccgagc cgcgtctgca atcggcgccc
     1681 ggcttcggcg ttgacggcga aaacggtcga atcaatctgg agctgaaaga gccgcagtcg
     1741 gaatggtggg agctggaaag aaagtggaac gccgagctgg caaacggaca gaaagaccag
     1801 aaaaccggca cggtcataac gctgccgatt gaagaagcca agaaccggct gatgcaggaa
     1861 aacattaagg cgcgacccgc cgagcaggga caaaaagctc tggaagaatc gcgctcgatt
     1921 atatcgtatt cgagcgccgg tcgcgtggcg aacgaaaaaa gaaggtaacg ggatttcgga
     1981 ttttcgattg ccgattttcg attgaagaag gaatttttta tttatgcggc gcgagcccga
     2041 ttccgatttc aatctaaaat cgccaaatct gaaatctgaa ataaagataa ggtttttatg
     2101 aaaaaaagtt cagcaatctt agcagtttta aggctgaagc ttttagtaag tttttgcatt
     2161 gcagcgtttg cggcgtttgc ctacgggcag gggcagcatt ataattcgcc gctttactcg
     2221 ccgcgcactt atgacccggc gcagggcacg accagcaacg gcttgccgaa gaccctgcag
     2281 gaagtcggca tcgagcaaag gctgaacgaa ccgcttccgc tcgatacgga gtttaaggac
     2341 gaaaacggca aaatcgtcaa gctcggcgaa tatttcggaa agcagcgccc cgtgattctg
     2401 gcgctggttt attacgaatg cccgatgctc tgcaacgaag ttctgaacgg tttgaccggc
     2461 tctttgaaag gcaattcgct cgacgccgga aaggatttcg acgtcgtcgc catcagtttc
     2521 gacgcgcgcg aatacgataa gcccgattta gcgaaaaaca aaaaagccgg ttatatgcgg
     2581 cgctacgacc gcgccggaac ggaaaacggc tggcattttc tgaccggcac gcaagagtcg
     2641 attgacagcg tcacgcaggc ggtcggcttt tcatatgaat gggacgagcg ctcgcagcaa
     2701 ttcgcgcata tgggcggcat tatgattgcg acgcccgagg gcaaactggc gagatatttt
     2761 tacggcatcg actacgcgcc gctcgatttg aaattaggct tgatggattc ggcgcagaat
     2821 aaaatcggca cgaaggtcga ccagctgatg ctttattgct atcactacga cccttcgacc
     2881 ggcagttacg gttttgcgat tttgcgcgcg atgcgtctgg cggcggtgct gactttgctc
     2941 ggaatcgggt cgatgctttt cgtcttttgg cgtaggaaca agaaaaaggt tttagttaat
     3001 ggttagctgt taatggattt cgacaaacaa aatcgccatt gacagttaac agaaaaagtt
     3061 atgcagcaag agaataacta tattccatta tttcccgaac aggcgtccac ttttgcgtgg
     3121 caggtggatg ctttatacgt ctacctgatt ttaatcagcg tgtttttcac ggttctgatt
     3181 gtcgccggaa tcttttactt tatggtcaga taccgcgagc gggaaaaatt tgaaaacggc
     3241 gcggaaattc acggctcgat gatgctggag acgacctggt cgataattcc gttcgtcatt
     3301 tcgatgacca tctttctggg cggcgccatt gtttattaca atcaatatcg cccgccgaag
     3361 gacgcgatgg aaatttacgt cgtcggcaaa caatggatgt ggaagattca gcacgccacc
     3421 ggacagcgcg aaatcaacga gctgcacgtg cccgtcggtc acaaaatcaa attaacgatg
     3481 acgaccgagg acgtgctgca cgatttttcg attcccgctt tccgaacgaa aatggacgtc
     3541 gtccccggac gctacacgac gctctggttc gagccgacca agccgggtaa atatcatctg
     3601 ttctgcgccg aatactgcgg cttgaaccat tcgggaatgg gcggctgggt ttacgtgatg
     3661 gaacagcgcg aattcgacaa ctggctttcc ggaaatactt ccaatcaaac gccggtcgaa
     3721 gccggaaagg aattgtttga agccaagctc ggctgcgcgt cgtgtcacgc cggcggaccg
     3781 aaccagcgcg gcgcgaaggt cgaaggcatc ttcggaacgc agatcaagct ggtcggcggg
     3841 caaaccgtcc tcgccgacga agcgtatatc cgcgagtcga ttttgaatcc gggcgcgcag
     3901 attgtcgaag gctatcagcc gattatgccg acctttaaag gacaggtttc ggaagaacag
     3961 cttgtcgctc tggtcgctta tattaaatct ttaagcggga ataacgccgg aagcgggaac
     4021 acgagcacga cgacgacaac ctcgacttcg accacgacaa atagcaacgg cgccagcaga
     4081 aacgaagtaa tgagtcctgc ctcgcgacga accgacacgg tcgcgccgat tacggacaga
     4141 ggacagtcaa acactcaggg cagcaatccg aacgcgccca gaaacagaag cgaaccgaca
     4201 aaccagccgc gataaatcgg cggctcagcg acatagaagg gtagtaaaca gatatggaaa
     4261 acgtagaagc aatgaacgtc ggcacaatca gaaccacgcc ggaaaagaat tatttgaaca
     4321 acggctacac tatcgcgtcc tggcttttga ccaaagacca caagcgcatc gcgataatgt
     4381 atttgataac cgtctcgatg ttttttatgg cgggcggact ctacgcctcg gcgattcgcc
     4441 tggagctgct gacgccgccg agcgatttgt tcgaatcggg aacgtataac aaggtcttta
     4501 cgcagcacgg catcattatg gtgttctttt tcctgattcc ggcgattccg gcaattctgg
     4561 gaaattttct gataccgatt atgatcggcg cgaaggattt ggcgtttccg cgcatcaatc
     4621 tgctcagctt atatttttac tggctcggcg gcttactgac gatttgggcg ctgcttcagg
     4681 gcggcgtcga taccggctgg actttctaca cgccgtacag cacgacgttt tccaatacat
     4741 acgtgatggc ggtcgggctc ggtattttta tcaacgggtt ttcgtccatc ctgacgggct
     4801 tgaactttat cgtcacgacg cacacgatgc gcgcgccggg aatgacctgg ttcagactgc
     4861 cgctttttgt ctgggcgcat tacgcgactt cgctggtgat gcttctggga acgccggtca
     4921 tcgcgattac gattctgctg ctggctctgg aacggctgac ccgcgtcgga attttcgacc
     4981 cggcaatcgg cggcgacccg attctgttcc agcacctttt ctggttttac tcgcatccgg
     5041 cggtttacat tatgattctg cccggcatgg gcatcatctc ggagctgatt tccaatttct
     5101 cgcgcaagaa aattttcggc tacgaattta tcgcgttttc ttcgattgcg attgcggttt
     5161 tcggctttct cgtctgggga caccatatgt ttgccagcgg tcagtcgatt tacgctgggc
     5221 tggttttctc gtttttgacg atggtcgtcg ccgtgccgtc ggcgattaaa gttttcaact
     5281 gggcggcgac gctttacaaa ggtcacatct cctacgatac gccgatgctt tacgccctgt
     5341 tttttatcgg tttgttttta atcggcggct tgaccggact ttttctcggc gctatgggat
     5401 tggacgttca cctgaccgat acttatttcg tcgtggcgca cttccactat gtcatggtcg
     5461 gcggtcaggt tttggcttac ctgggcggca ttcactactg gtggtcgaag atgacgggca
     5521 gaatgtattc ggaattctgg gcgaaaatcg cggcgatgat tgtcttcgtc ggtttcaacc
     5581 tgacattctt cccgcagttt attctcggtt atctgggaat gccgcgccgc tatgcgtcct
     5641 atccgccgga gatgcaggtc ttgaatatct tttcgacggc
//
LOCUS       DASWNE010000471        10127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_474614, whole genome shotgun sequence.
ACCESSION   DASWNE010000471 DASWNE010000000
VERSION     DASWNE010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10127
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474614"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            251..763
                     /locus_tag="VGB68_09345"
     CDS             251..763
                     /locus_tag="VGB68_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049476.1"
                     /translation="MKKLALIFSIIAIFSASAFAQKADDSKDALAVVNKLFAEMAAHN
                     PAGIVALHMPEAQLAAAIKTKEGKSVIRSFAAEAFSKNFAEKKSELKEEMYAPETKIF
                     GDLALVWGRYVFFVDGKISHCGVNAFHLVRTDAGWKIANASTTIEPQGCTEQEKAMKV
                     AAPTTTTTRQ"
     gene            951..2450
                     /locus_tag="VGB68_09350"
     CDS             951..2450
                     /locus_tag="VGB68_09350"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY0049477.1"
                     /translation="MNHKKHGPLLAVILLFSFSAAAAQTNGFAPVNSVKKELAIERLK
                     KNVPELMKEARIPGLSLALIRGGKLVWSQGFGVKNSETKEAVGGDTIFEAASLSKPVV
                     AYAVLKLVDAGKIDLDVPLNKYLPGNYDAGDDARINQITTRNVLSHTSGFPNWRSPRN
                     AKTLPIFFTPGERFSYSGEGFVYLSKVVEHITKTKLEDFIRRTVFEPLGMNASSFVWQ
                     EKYKTLKGFNHDIIGTPTGRNEPEEANAAASLHTTAADYARFVIAVLEGEGLKKPTRN
                     LMLSPQVRTDETCLVCARPATKLSTELGWGLGWGLQTTDEGTSFWHWGDNGNNKSFIV
                     AFDKQKSGIVVLTNSANGLSIIKEILADGVGGKYPALAWIKYESFESPGRILLKAILN
                     EGAEKALRDYRQKREKTAEIRLDESQTNSLGYDLLRARKIDEAIAVFHLNTEDFPKSA
                     NVWDSLGEAYAVKGDKRKAIENYKKSLQLNPENKNAADRIKLLEQPPQQ"
     gene            2481..3935
                     /locus_tag="VGB68_09355"
     CDS             2481..3935
                     /locus_tag="VGB68_09355"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0049478.1"
                     /translation="MRNGKRRWIQKCDVDEKNLPIPTQIVSNEEFAPPAQTPAQKRVE
                     QRLIEIASDTSKRLGISRRRFLAASGGMAAAFLAMNSVFGKFFDVEESELFEPQATDE
                     KFPKNQFIFDIHTHHVAAKKIIQTPNLLNYRAAGAAWGNKNLQGKEHRWEDLYLANYI
                     KEMFLDSDTVMAVITGLPAKSDAENVLPPAEMIETRAEINGLAKSRRIIAHGLFSPDL
                     GKRDMEAMQRQFEKMKVEAWKGYPGQPLADGSVGWWMDDEKIAYPAYEYSRRIGIKNI
                     CVHKGLPLPGWDLEHSSPKDVEKAAKDFPDLNFLIYHAGFKGVRDALEAVKDGFKTKT
                     DVPWISDLCAMRKRNPKMTNVYMDLGTTFGMTVITEPLLCAFMLGLMIDAFGEDHVLW
                     GTDSIWWGSPQWQIEAFRRLQMPEDLMKRFKFKPLTAAVKAKILGLNSAKVYGVDAKA
                     KMNAIPSDYMTRLKEKYRAAGAQPSNTQYGWIFD"
     gene            4018..5823
                     /locus_tag="VGB68_09360"
     CDS             4018..5823
                     /locus_tag="VGB68_09360"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0049479.1"
                     /translation="MKNLLFVFILISFGFIAIRAQTVAFTGVNVIPMDKERVLQNQTV
                     LVRDGVIAEIGKNVKIPAGAQIIDARGKYLIPGLVDMHTHLLSDGDDYPNSIAEDELR
                     VMVANGVTTIRFMIGTPEQLVLRRKSASGEIVAPTIYSASPHLTGKEQGNNFVVNTAE
                     EAREAVRKSKAAGYDFIKITTFIKAEVYEAAVDEAAKQNIRVVGHADARFVGVQRAFK
                     AKQQIEHLDGYMEAIVADNAPSKDSISDLYIYNPKNWESLDYVDEKKIAQVAKQTVAA
                     NPFVDPTQHFMKNTFGLPRSEESIRAQPDFRFYPKKVQEGFFNFYKRSRFINQVSYEK
                     RARWVELRNKIIKAIYDAGGKIMTGSDTPEFLWLYGFTMHREMKALSDAGLSNYAVLE
                     AATKNPSMFFGTIEKVGTIEKGRRADLVLLDANPLENISNTEKRAGVMLKGKYYAQEE
                     MSRWLDEIAPRISNSFIEKPNPQQEAAAVADKLFEAMRAKNADAIRALFTAEGQLIAV
                     DKPRGGEGISKTRVFTADAFAKMISEAKGELIEKMPEKDVKISGDFALVTGRYTFHVG
                     DKFSHCGVNSFQLVRTESGWRIANAASTLEFACGQ"
     gene            5841..7190
                     /locus_tag="VGB68_09365"
     CDS             5841..7190
                     /locus_tag="VGB68_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049480.1"
                     /translation="MKSKRAENSGFKPRARKIFSASAAIAACFILLALFSINLPAQEN
                     SALARAQQFENQAREAFEARDFAAYLANVKQAAELRPNHPRLLYNLARAYALNDNRPE
                     ALRLLERLAKMGMLTRLASDPELEKQLPASDFQNLKNLFERNRQPLNASSRAFSLPEK
                     DLITEGVAYDSVSKRFFVSSVRQRKILVVTRDGETSEFSSPQDNLWSVLGMKTDEKRR
                     LLWVCTTAFPQMPGFKKTDEGVSAIFKYDLNSGRLLKKYVLSNASGKHGLGDLVAARN
                     GEVFATDSISPAIYRINPKSDEIEPFLLDKSFASLQGLTFAPGEKHLFVADYSLGIFS
                     VEMATKKITWLAPASEVVALGVDGLYFHQGKLIGVQNGTNPQRIVRFSLSRDNLRLTR
                     TETLEANHADFNEPAHGVLVGRDFYFIANSQWEMVDEKGETGAPEKWREPVVLRLRL"
     gene            complement(7631..8113)
                     /gene="dps"
                     /locus_tag="VGB68_09370"
                     /gene_synonym="pexB"
     CDS             complement(7631..8113)
                     /gene="dps"
                     /locus_tag="VGB68_09370"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: protein motif:HMM:NF006975.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HEY0049481.1"
                     /translation="MELHKTKNGLSQETREKVIDILNARLADASDLKSQAKQAHWNVK
                     GIHFFQLHELFDQVATAVEAHMDLIAERATALGGTALGTVRVAAQNSSLSEYPLEITD
                     GTAHVDALSSALADFGNVVRRNIDETNDLGDADTADLFTEVSREIDKLLWFVEAHIQA
                     "
     gene            8235..9125
                     /locus_tag="VGB68_09375"
     CDS             8235..9125
                     /locus_tag="VGB68_09375"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEY0049482.1"
                     /translation="MQNSNTSRIIENAWTIKAVTYESINNIVASIFERLPYILAGFLV
                     LGIFFVLARIARLIFWTASGRTKLDNRLRILFSRLLVVFIVVLGIFTALTVVVPTFGF
                     GDMITGLGFTSFVIGFATKDILNNLLSGVLILWQQPFRIGDYLFVGNNQGKVEYIGVR
                     ATQLRKDDGELILIPNGDMYTNALTIRSASANRRMNLKISIGYESDIERARKTIKNAM
                     QSVEGVVEEPKPNVYVTDLSSDGVNLAIYFWINTHEAKPMEVFDRVATEIKNSLSRAS
                     VEMYPPNTVIVQNADTQIDS"
     gene            9329..9541
                     /locus_tag="VGB68_09380"
     CDS             9329..9541
                     /locus_tag="VGB68_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049483.1"
                     /translation="MSKDVISVDGEDRVVREDTAKSYRGVIWALTSIAAFIIIVAILV
                     FGGFLSTATDGSSTKSPAEIEKQRQQ"
     gene            9829..>10127
                     /locus_tag="VGB68_09385"
     CDS             9829..>10127
                     /locus_tag="VGB68_09385"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049484.1"
                     /translation="MLVLGIESSCDETAAAITRDGKEILSSVISSQIDLHRPYGGVVP
                     ELASREHLNKIEPIVLEALKQADIELKDIDAVAVTQGPGLVGSLLVGVCYAKALA"
BASE COUNT         2671 a         2404 c         2648 g         2404 t
ORIGIN      
        1 caacccgctt ccgaaagagt ttctgccgta aaagaatttg cccgtcaaag gcgcgaaagg
       61 gcgcgaaaga aaaatgaaac cacagatgaa cgcggataaa cgcagatttt tgaaagataa
      121 aagcggatta attttatcct gcgtctatcc gcgtctatcc gcttttatct gtggttaact
      181 ccctttcttt tttcgtttgt ttcgcgtgtt tcgcgggctg aaaaatatta aataaggacg
      241 aattaaaaaa atgaaaaaac tagcacttat cttttcaatt atcgccattt tctcggcttc
      301 ggcttttgcc cagaaagccg acgattcgaa agacgcttta gccgtcgtca acaaattgtt
      361 tgcggaaatg gcggcgcaca atccggcggg aatcgtcgcc cttcatatgc ccgaagccca
      421 gctggcggcg gcgattaaaa ccaaggaagg gaaatccgtc atcagaagtt ttgccgccga
      481 agcgttttcc aaaaatttcg cggaaaagaa aagcgagctg aaggaagaga tgtacgcgcc
      541 ggaaaccaaa atcttcggcg atttggcgct cgtctggggc agatacgttt tttttgttga
      601 cggcaaaatc tcgcattgcg gcgtcaacgc gtttcacctg gtgcgcaccg acgcgggctg
      661 gaaaatcgcc aacgcttcga cgaccatcga gccgcagggc tgcaccgagc aggaaaaagc
      721 gatgaaagta gccgcgccga caacaacgac gacccgacag taattttttt taccgaccgg
      781 aataaaattt attcgtattc cgaaaggcga aatcgagcag ctttttatgg gtctggacgc
      841 ggcgcgaaaa atttctcgga aagattttcg gcgagcgacg ccggcgtgaa atgtcacaaa
      901 agcgctttag ttggtgtttt caatataaag ccgaaaattc ggaggtcgtt atgaatcata
      961 aaaaacacgg accattgctc gcggtaattt tacttttctc tttttccgcc gccgccgcgc
     1021 agacgaacgg gtttgcgccg gtcaacagcg tcaaaaaaga attggcgatt gagcggctga
     1081 aaaaaaatgt ccccgaattg atgaaagaag cgcggattcc agggctttcg ttggcgctga
     1141 ttcgcggcgg aaagcttgtt tggagtcagg gcttcggcgt caaaaactcg gagacgaaag
     1201 aagcggtcgg cggcgatacg attttcgaag ccgcgtcttt gtcaaagccg gtcgtagctt
     1261 acgcggtttt gaagctcgtc gacgcgggca aaatcgattt ggatgtgcct ttaaataaat
     1321 atctgccggg caattacgac gcgggcgacg acgcgcggat taatcagatt acgacgcgaa
     1381 acgttttgag ccacacgagc ggatttccga actggcgctc gccgcgcaat gccaaaacat
     1441 tgccgatttt tttcacgccc ggcgagcgct tcagctattc gggcgaaggc ttcgtttatc
     1501 tttcaaaggt cgtcgagcac atcacgaaaa cgaagctcga ggattttatc cgccggacgg
     1561 ttttcgagcc gctcggaatg aacgcgagca gctttgtgtg gcaggaaaaa tataaaactc
     1621 tcaaaggttt taatcacgac attataggca cgccgaccgg acgaaatgaa cctgaagaag
     1681 ccaacgccgc cgcaagtctg cacaccaccg ccgccgatta cgcgcgattt gtcatcgccg
     1741 ttttagaagg cgaaggattg aaaaagccga cgcgaaattt gatgctctcg ccgcaggtca
     1801 ggacggacga aacctgtctg gtctgcgctc gtccggcgac gaagctttcc acagagctcg
     1861 gctggggact cggctggggc ttgcagacga ccgacgaagg cacgtctttc tggcactggg
     1921 gcgataatgg caacaacaaa tcttttatcg tcgcctttga taaacaaaaa agcggtatcg
     1981 tcgttttgac caacagcgcc aacgggcttt cgattatcaa agaaattctc gccgacggcg
     2041 tcggcggcaa atatccggct ctggcgtgga taaaatacga aagcttcgaa tcgcccggca
     2101 gaattctgct caaggcgata ctcaacgagg gcgcggaaaa agctctccgg gattaccgtc
     2161 aaaaacgcga aaaaacggct gaaatccggc ttgacgaaag tcagacgaac agtctcggct
     2221 acgatttgct gcgggcgaga aaaattgatg aggcaatcgc cgtttttcat ctcaatacgg
     2281 aagattttcc gaaatcggcg aacgtttggg acagcctcgg cgaagcttac gcggtcaaag
     2341 gcgacaagcg gaaagccatc gaaaattaca aaaaatcgct gcagctaaac ccggaaaata
     2401 aaaatgcggc agaccgcata aaactgctgg agcagccgcc gcaacaataa atttgtaaat
     2461 tatcaaaaga gctgaaaacg atgcgaaacg gaaaaaggcg atggattcaa aaatgcgacg
     2521 ttgacgaaaa aaatctgccg ataccgacgc agatagtttc gaacgaggaa tttgcgccgc
     2581 ccgcgcagac gcccgcgcag aaacgggtcg agcagcgcct gattgaaatc gcctcggata
     2641 cttcaaagcg tctgggcatc agccgtcgcc ggtttctcgc cgcatcgggc ggaatggcgg
     2701 cggcttttct ggcgatgaat tccgttttcg gaaaattttt cgacgttgaa gaaagcgagc
     2761 tgttcgagcc gcaggcgacg gacgaaaaat ttccgaaaaa ccagtttatt ttcgatattc
     2821 acacgcatca cgtcgccgcg aagaaaataa ttcaaacgcc gaatcttttg aattaccggg
     2881 cggcgggcgc ggcttggggc aataaaaatt tacagggaaa ggagcaccga tgggaggacc
     2941 tatacttagc gaattatatt aaggaaatgt ttctcgacag cgacaccgta atggcggtca
     3001 tcaccggact tccggcgaaa tcggacgccg aaaacgtttt gccgcccgcc gagatgatcg
     3061 agacgcgcgc cgaaatcaac ggcttggcga aatcgcgccg cattatcgcg cacgggcttt
     3121 tctcgccgga tttgggcaag cgggatatgg aagcgatgca gcggcagttc gagaaaatga
     3181 aagtcgaagc gtggaaaggc tatcccgggc agccgctcgc cgacggcagc gtcggctggt
     3241 ggatggacga cgaaaaaatc gcttacccgg cttatgaata ctcgcgccga atcggaatca
     3301 aaaacatctg cgttcacaaa ggcttgccgc tgcccggctg ggatttggaa cattccagcc
     3361 cgaaagacgt cgaaaaagcg gcgaaggatt ttccggattt gaattttctg atttatcacg
     3421 ccggttttaa aggcgtgcgc gacgctctgg aagcggtcaa agacggtttc aaaaccaaaa
     3481 ccgacgttcc gtggatttcc gatttatgcg cgatgcgaaa gcgaaatccg aaaatgacga
     3541 acgtttatat ggatttggga acgacgttcg ggatgacggt tatcaccgag ccgcttttgt
     3601 gcgcctttat gctcgggctt atgattgacg ctttcggcga agaccacgtt ttgtggggca
     3661 cggattcgat ttggtggggc agcccgcaat ggcaaatcga ggcgttcagg cgtctgcaaa
     3721 tgccggaaga tttgatgaag cgtttcaagt ttaaaccgct gaccgctgcg gtgaaagcaa
     3781 aaattctggg tttgaattcg gcgaaagttt acggcgtcga cgcgaaggcg aaaatgaacg
     3841 cgattccgtc cgattatatg acgaggctca aggaaaaata tcgcgccgcc ggagcgcagc
     3901 cttcgaacac gcaatacggc tggattttcg attaagcttt gagttttgta ttttgaatac
     3961 ttcgttttat aaaaaactca aaactccaca ttcataattt cttttgagag gttgatgatg
     4021 aaaaatctat tatttgtttt tattctgatt tcattcggtt tcatagcgat acgagcgcag
     4081 acggtcgctt ttaccggcgt aaacgtgatt ccgatggaca aggagcgcgt tttgcaaaat
     4141 caaacggtgc tcgtccgcga cggcgtcatc gccgaaatcg gcaaaaacgt aaaaattccg
     4201 gcgggcgcgc aaattattga cgccagaggg aaatatctga ttccgggatt ggtcgatatg
     4261 cacacgcatc tgctttcgga cggcgacgat tatcccaatt cgattgccga agacgagctg
     4321 cgcgtgatgg tcgccaacgg cgtgacgacg attcgcttta tgatcggcac gccggagcag
     4381 ctcgttctgc gccgaaaatc ggcgtcgggc gaaatcgtcg cgccgacgat ttactcggca
     4441 agcccgcatc tgacagggaa agagcaggga aataattttg tggtcaatac cgcggaagaa
     4501 gcgcgcgaag ccgtcagaaa atcgaaagcg gcgggttatg atttcatcaa aataacgact
     4561 tttatcaagg cggaagttta cgaagccgcc gtcgacgaag ccgcgaaaca aaacatccgc
     4621 gtcgtcggtc acgccgacgc ccgattcgtc ggcgttcagc gggcgttcaa agcgaaacag
     4681 caaatcgagc atctcgacgg ttatatggaa gccattgtgg cggacaacgc gccgagcaaa
     4741 gattcgattt ccgaccttta catctacaat ccgaaaaatt gggaaagcct cgattacgtt
     4801 gacgaaaaaa agattgccca agtcgccaaa cagacggttg cggcgaaccc gtttgtcgac
     4861 ccgacgcagc attttatgaa aaacactttc ggtctgccgc gcagcgaaga atcaatccgc
     4921 gcccagcccg attttcgttt ttacccgaaa aaagtgcagg aaggtttttt taatttctac
     4981 aagcgcagcc ggtttatcaa tcaggtttcg tatgaaaaac gcgctcgctg ggtcgagctc
     5041 agaaacaaaa tcatcaaggc gatttacgac gcgggcggaa aaattatgac cggctcggac
     5101 acgcccgaat ttttgtggct ttacggcttt acgatgcatc gcgagatgaa agctctgagc
     5161 gatgcgggct tgtcgaatta cgccgttctg gaagcggcga cgaaaaatcc gtcgatgttt
     5221 ttcggcacga ttgaaaaggt cggcacgatt gaaaaaggca ggcgcgccga tttggttttg
     5281 ctcgatgcca atcctttgga aaatatctcc aacaccgaaa aacgcgcggg cgtgatgctg
     5341 aaaggaaaat attacgcgca ggaggaaatg agcagatggc tggacgaaat cgcgccgcga
     5401 atctccaatt cttttatcga aaaacctaac ccgcagcagg aagcggcagc cgtcgccgat
     5461 aagctttttg aagcgatgcg ggcgaaaaac gccgacgcca ttcgcgcctt gttcacggcg
     5521 gaaggtcagc taatcgccgt cgataaaccg cgcggcggcg agggaatttc gaagacgcgc
     5581 gtatttacag ccgacgcttt cgccaaaatg atttccgaag ccaaaggcga acttatcgaa
     5641 aagatgccgg aaaaggatgt gaaaatcagc ggcgatttcg cgctcgtcac cggacgctac
     5701 acttttcacg tcggcgataa attctcgcat tgcggcgtca attcctttca actggtcaga
     5761 accgaaagcg gctggcgcat tgccaatgcc gcttcaacgc tggaatttgc ctgcggtcaa
     5821 tagatttcag cggaagtttt atgaaatcaa aacgcgcaga aaattcaggt ttcaagccgc
     5881 gcgcccggaa aattttttcc gcctccgccg ccatcgctgc ctgtttcatt cttctcgcac
     5941 ttttttcgat aaaccttccg gcgcaggaaa acagcgcttt ggctcgcgcg cagcaatttg
     6001 aaaatcaggc gcgcgaggct ttcgaggcga gagatttcgc cgcgtatctc gccaacgtca
     6061 aacaagccgc cgagctgcgc ccgaatcacc cgcggctgct ctacaatctg gcgcgggcgt
     6121 atgcgctcaa cgataaccgt ccggaagcgc tgcgcttgct ggaacggctg gcgaaaatgg
     6181 gaatgctgac gcggctcgca agcgacccgg agttggaaaa acaactgccc gcgagcgatt
     6241 ttcaaaacct gaaaaatctt ttcgaacgta atcgccagcc tttaaatgcc agttccagag
     6301 catttagctt gccggaaaaa gatttaatca cggaaggcgt cgcttacgat tccgtctcta
     6361 aacgcttttt cgtcagcagc gtccggcaaa gaaaaattct ggtcgtcacg cgtgacggcg
     6421 agacgagcga gttttcgtcg ccgcaggaca atctgtggag cgttctcgga atgaaaacgg
     6481 acgaaaagcg ccgcctgtta tgggtttgca cgacggcttt cccgcagatg cccgggttta
     6541 agaaaacgga cgaaggcgtt tcggcgattt tcaaatacga tttaaacagc gggcggcttt
     6601 tgaaaaaata cgttttatcg aacgcgtccg gcaaacacgg tctgggcgat ttggtcgcgg
     6661 ctcgaaacgg cgaagttttc gcgaccgaca gcatttcgcc cgcgatttac cgcatcaatc
     6721 cgaaatcgga cgaaatcgag ccttttctgc ttgataaatc tttcgcctcc cttcagggat
     6781 tgactttcgc tccgggcgaa aaacatcttt tcgtcgccga ttattcgctg ggaattttca
     6841 gcgtcgaaat ggcgaccaag aaaataacgt ggctcgcgcc ggcaagcgaa gtcgtcgcgc
     6901 tcggcgtcga tggactttat tttcatcagg ggaaattaat cggcgtgcag aacggcacaa
     6961 atccgcagcg aatcgtgcgt ttttccttga gcagagacaa tctgcgctta acccgaaccg
     7021 aaacgctcga agccaatcac gccgatttta acgagccggc gcacggcgtg ctcgtcggtc
     7081 gggattttta ttttatcgcc aacagtcaat gggaaatggt cgacgaaaaa ggcgaaaccg
     7141 gcgcgcccga aaaatggcgc gagccggtcg ttctgcgtct gcgtttatga gctttaattt
     7201 ttcgcgcgcc tcgaagactt cgcgttttat ttttaaacct gaggtttgcc gaaacgtcta
     7261 ttccggcgcg attgtcggaa agcacaaatt aaataaaagg ggcgcggaga aaaggacgca
     7321 gagcgaagcg gcatttttga aataaagaaa caacggatta agaggagcga ctacgaatta
     7381 cttaaagaat ccatctcaaa ttttgttttt tttcggcgtt tcgccgcgct ccgggcggtg
     7441 aatgattaaa tttatgaaaa cttgatttat aaaaatcttt tcgattgttt tcgttttcgt
     7501 ttcgccgcgg ttcacggagc ggcggcggtc gccgaaaagg aaaaactcgg aaatagagtt
     7561 tttatttgct aaaaaaagcg atgcctgacg taatttacgt cgagcatcgc tttttgattt
     7621 ttgcgaaaaa ttaagcttga atgtgcgcct cgacaaacca taaaagcttg tcgatttcgc
     7681 gcgagacttc cgtgaaaagg tctgccgtgt cggcgtcgcc caaatcgttg gtttcgtcta
     7741 tgttgcggcg aacgacattg ccgaaatcag ccagcgcgct cgaaagcgcg tcgacgtgcg
     7801 ccgtgccgtc cgtaatttcc agcggatatt cgctcaaaga agaattctgc gcggcgacgc
     7861 gaaccgttcc gagcgccgtt ccgccgagcg ccgtcgctct ttccgcaatc aaatccatat
     7921 gtgcttcgac cgccgtcgcc acctggtcga aaagctcgtg cagttgaaaa aaatgaattc
     7981 ccttgacgtt ccagtgcgct tgtttagcct gcgatttcaa atcgctcgcg tcggctaaac
     8041 gcgcgtttaa aatgtctata actttttcgc gcgtctcctg cgataacccg tttttcgttt
     8101 tatgtaattc cataattcct ccgatttaga attattctaa ttctaaaata atcgaatgtc
     8161 aaaccgccgg ataaagtatc caaccttttg ctaactataa gcatatatct atatttatta
     8221 tgtttactat gttaatgcaa aactcaaata cttcaagaat tatcgaaaac gcgtggacta
     8281 tcaaagccgt cacatacgag tcaatcaaca acatcgtggc ttcgatattc gagcgtctgc
     8341 cttacattct ggcgggattt ctggtgctcg gaatcttttt cgtgcttgcc agaatcgcca
     8401 gattaatttt ctggacggct tccggcagga cgaaactgga caatcgcctg cgaattttgt
     8461 tcagccgtct gcttgtcgtt tttatcgtcg tgctgggcat ttttacggct ttgacggtcg
     8521 tcgttccgac tttcggtttc ggcgatatga taacgggctt gggctttacg tcgttcgtca
     8581 tcggttttgc gacgaaagat attctgaaca atcttttatc gggcgttctg attttgtggc
     8641 agcagccgtt tcgcatcggc gattatcttt tcgtcggcaa caatcagggc aaggtcgaat
     8701 acatcggcgt gcgcgccacg caattaagaa aagacgacgg cgagctgatt ctgattccga
     8761 acggcgatat gtataccaac gcgctgacga ttcgcagcgc gagcgccaat cggcgaatga
     8821 atctgaaaat cagcatcggt tacgaatccg atatagaacg ggcgagaaaa actattaaaa
     8881 atgcgatgca atcggtggaa ggcgtcgtcg aagagccgaa accgaatgtt tacgttaccg
     8941 atttatcttc ggacggcgta aacctggcga tatatttctg gataaacacg cacgaagcga
     9001 aaccgatgga agtttttgac cgcgtggcga ctgaaatcaa aaactctttg agccgcgcca
     9061 gcgtcgaaat gtatccgccg aacacggtta tcgtgcaaaa tgcggataca caaatagata
     9121 gctgaattgt ttccggcttg tacggattca gtccagcgtt gtttggaaat aatacaagca
     9181 ggcgttttcg gtaaaaaacg caacatccgg ctttaagcct tgtattttcg gtaaatttat
     9241 cgtttcgaac ctttggcacg ctcattgctt taatagatgc gtctgctgct gcggatgggc
     9301 ggattatttg ataagggaga tacggaaaat gtctaaagac gttattagcg tggatggcga
     9361 agacagagtc gttcgcgaag atacggcaaa gtcttacaga ggagtcatct gggctttaac
     9421 cagtatcgca gcatttatca taattgtagc aatcttggtt tttggcggat ttttaagtac
     9481 ggcgaccgat ggcagttcta caaaaagtcc ggctgagatt gaaaaacaaa gacagcaata
     9541 atagttttta cgaacaaggg attttaaacg agcaaggggt tatcaaacga gcaaggggta
     9601 ggtttaggtt ttcaacgagc aaggggtagg ttaatcggtg tgtagttctt tcctgtcatg
     9661 tagaactttt tagtcataag tggacattgt caatttttga cttaaaacgt cccctgaaat
     9721 ttcaggggac gttttttttg taggaataac taatagttga taaatgatag ttgttttgct
     9781 ttcactatca gttatcgact atcagttatt ttgctttaca ctaaaagaat gttggtttta
     9841 ggcattgaaa gctcatgcga cgaaacggcg gcggcaatca cccgcgacgg gaaagaaatt
     9901 ttatcgtccg ttatttcctc gcagattgat ttgcatcgtc cttacggcgg cgtggttccc
     9961 gagctggctt cgcgcgagca tctaaacaaa atcgagccga ttgtgctgga agctctcaaa
    10021 caggcagata ttgagttaaa agacatcgat gccgtcgccg tcacgcaagg tcccgggctg
    10081 gtcggctcgc ttctggtcgg cgtttgttac gcaaaggctc tggcgtt
//
LOCUS       DASWNE010000472         1717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_227631, whole genome shotgun sequence.
ACCESSION   DASWNE010000472 DASWNE010000000
VERSION     DASWNE010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227631"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            506..1663
                     /locus_tag="VGB68_09390"
     CDS             506..1663
                     /locus_tag="VGB68_09390"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEY0049485.1"
                     /translation="MSTNKINRRAIEIRRHREIRASDFKSFRFAVGALFLCALIIFSF
                     TYQKPESIERAVVVEPERITESRVTFLAVGDMMISRGVARSITRANDPLAPFRKMEAV
                     FRSTDFNFGNLEVPISGNNNVIGKGLVFNMHTRDIAGLKAYNFKVLNLANNHALDQGV
                     EGLRRTQRFLDEQGFTYLGVGDNPEQAWQPKTITVNNVKIGFVGASYASINDGGVARN
                     DYVARIEDTDRLQRAIARLRAEGADFIVATMHAGVEYTRRPHQPQIDFARNAIDSGAD
                     IVIGAHPHWTQIFETYKGKYIFYSLGNFIFDQEWSRDTKEGLALKITLQNRKSSAPKS
                     ASALQGTRVEQIELIPVVIENFSTPRPATETEAQQILKKIGATEKIIKPQM"
BASE COUNT          459 a          429 c          406 g          423 t
ORIGIN      
        1 atgattatgg attgtttgtt tacgcaaatg atgataattg gttttaccaa tctttgcaag
       61 cttattttaa agttttttca gatattttca gatactattt gattttgttc agcaaattcg
      121 atattttcga tttgatttcg acaataaagc cgagcttcgc tcacgacccg tcgccttacc
      181 aatttaagtt tttaacttga tgggttttac caattcataa atattcaaat atttttattg
      241 tttgaaaatc gagcccgcaa tcaatccgtc cggctctttt taccaatgcc tgcggcggtc
      301 ggcggcggct ggaaaaaagc ctttattaaa tatttgaagt ttaaaaaaaa tcagattttg
      361 attgacttta aacgataaag tgcagtataa gaatttccat atcatacagg gcgatttaga
      421 tttatataaa attatcgggc ggcgcgaagg caaaaaactt cggcattatc gtccgacgat
      481 aataggaaat ttgcgtgtga tttgaatgtc aactaataaa attaaccgcc gcgccataga
      541 aatcagaaga cacagagaaa ttcgagcctc ggattttaag tcttttcgct tcgccgtcgg
      601 cgcgctgttt ctctgcgccc tcatcatctt cagctttact tatcaaaagc cggaatcgat
      661 tgagcgcgcc gtcgtcgtcg agccggagcg gattacggag agccgcgtca cgtttctcgc
      721 cgtcggcgat atgatgattt cgcgcggcgt cgcgcgctca atcacgcggg cgaacgaccc
      781 gctcgcgccg tttcgaaaaa tggaagccgt ctttcgctcg accgatttta acttcggcaa
      841 tctggaagtg ccgatttcgg gcaacaataa cgtcatcggc aagggattgg tctttaatat
      901 gcacacgcgc gacatcgccg gattaaaggc ttataacttt aaagtgctga atctcgccaa
      961 caaccacgcg ctcgaccagg gcgttgaagg cttgcgccgg acgcagcgat ttttagacga
     1021 gcagggcttt acatatctgg gcgtcggcga caatccggag caggcttggc agccgaaaac
     1081 catcaccgtc aacaatgtga aaatcggttt tgtcggcgcc tcatacgcct cgattaacga
     1141 cggcggcgtg gcgcgcaatg attatgtcgc ccgcatcgaa gatacagacc gcctgcaaag
     1201 agcgatcgcc cgattgcgag ccgaaggcgc ggattttatc gtcgccacga tgcacgccgg
     1261 cgtcgaatac acgcgccgcc cgcatcagcc gcaaatcgac tttgcccgca atgccatcga
     1321 ctccggcgcg gacatcgtta tcggcgctca cccgcactgg acgcagattt tcgaaaccta
     1381 caagggcaaa tatatttttt attcgctcgg caattttatc ttcgaccagg aatggagccg
     1441 cgacacgaaa gaaggactgg ctttaaaaat cacgctgcaa aaccggaagt cttccgcgcc
     1501 gaaatccgct tctgcgctgc aaggcacgcg cgtcgagcaa atcgaattaa ttcccgtcgt
     1561 catcgaaaat ttcagcacgc cgcgaccggc gaccgaaact gaagcgcagc agattttaaa
     1621 gaaaatcggc gcgaccgaaa aaatcatcaa gccgcagatg taaagacgaa acccggcgaa
     1681 atcagccgcg ccggatttca aaagataacc tgagcgg
//
LOCUS       DASWNE010000473         2748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_686391, whole genome shotgun sequence.
ACCESSION   DASWNE010000473 DASWNE010000000
VERSION     DASWNE010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686391"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VGB68_09395"
     CDS             <1..427
                     /locus_tag="VGB68_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049486.1"
                     /translation="SAVELAGAENFKGKTVIDVTNPLDFSEGIPPKFTASVGDSLGER
                     IQNALPEANVVKAFNTINRTTMIDPHFGAGETATLFIAGNDETAKAETIKLAEEFGWN
                     VEDLGDIGQAFFLEAFASLWVNYAFKHNSWQHAFKLLKK"
     gene            466..1557
                     /locus_tag="VGB68_09400"
     CDS             466..1557
                     /locus_tag="VGB68_09400"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HEY0049487.1"
                     /translation="MSKLFEPLKIREITFRNRIWVSPMCQYSSEDGMPTDWHLVHLGS
                     RAVGGAGLVIMEATAVSPEGRISPQDAGIWSDAHAEAYQRITKFIKENGAAAGIQIAH
                     AGRKASTAAPWNGGKKVDESAGGWQTLSPGASAFAEDYPTPREMNAADIEKAINDFAE
                     AAKRAVNAGFEVVEIHAAHGYLLHEFLSPISNQRTDEYGGSFENRARFPLEISRKVRA
                     AVPENLPLFVRISATDWTEGGWDLEQSIEFCRKLKEIGVDLIDCSTGGNLPQAKIPVA
                     PNYQVPFAAEIRRKAGILTAAVGMIAEPQQAEEILRKGEADAVMIARQFLREPYFPFR
                     AARELGAEIDVPKQYGRAIEIQTAKGAKR"
     gene            1653..2681
                     /locus_tag="VGB68_09405"
     CDS             1653..2681
                     /locus_tag="VGB68_09405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY0049488.1"
                     /translation="MSSFSNIPFSVLDLAPVVKGGSVGDALRDSLSLAQHAEKLGYRR
                     FWLAEHHNMTGIASAATSVVIGYVAGGTSKIRVGAGGIMLPNHAPIVIAEQFGTLETL
                     YPNRIDLGLGRAPGSDQATALALRRTLHTSGDDFPELLEELRFFLREPVAGQRVRAVP
                     GAGLHIPIWLLGSSGFSARLAGELGLPFAFAGHFSPEYILPALELYRNSFEPAGALEK
                     PLAMLAINAVAADTDEEAWRLASSQFQSFLNLIRGKPGQLQPPVENMDELWTPQEKAA
                     VESKLGGSIIGSAPTVKEKLEKILAETGADELMINAMIYDHAARLRSYEIVADVWQDK
                     TERYYVKK"
BASE COUNT          668 a          691 c          820 g          569 t
ORIGIN      
        1 aagcgccgtc gaacttgccg gcgcggagaa ttttaaagga aaaaccgtga ttgacgtgac
       61 caatccgctg gatttttccg aaggcattcc gccgaaattt acggcgtcgg tcggcgactc
      121 gctcggcgag cggattcaaa acgctctgcc cgaagcaaac gtcgtcaaag cgtttaatac
      181 aatcaatcgg acgacgatga tcgacccgca tttcggcgcg ggcgaaaccg cgacgctgtt
      241 tatcgccgga aacgacgaga cggcgaaagc cgaaacaatt aaactggcgg aagaattcgg
      301 ctggaatgta gaagatttag gcgatatcgg tcaggcgttt tttcttgaag ctttcgcctc
      361 cctgtgggtt aattacgctt ttaaacacaa ttcgtggcag cacgctttta agttattgaa
      421 aaaataaaaa gtcttcgaaa gagaaaatta acgagtaggg gaagtatgag caagttattc
      481 gagcctttaa aaataagaga aatcactttc agaaatcgca tctgggtttc gccgatgtgt
      541 caatattcga gcgaggacgg aatgccgacg gactggcatc tggtgcatct gggcagtcgc
      601 gcggtcggcg gcgccgggct tgtgattatg gaagcgaccg ccgtctcgcc cgaaggtcgc
      661 atctcgccgc aggacgccgg aatctggtcg gacgcgcacg ccgaagctta ccaaagaatc
      721 acgaaattta ttaaggaaaa cggcgcggct gccggaatcc aaatcgcaca cgccggacgc
      781 aaagcttcga ccgccgcgcc ctggaacggc ggcaaaaagg ttgacgagag cgcgggcggc
      841 tggcagacgc tgtcgccggg cgcgagcgcc tttgccgaag attacccgac gccgcgcgag
      901 atgaacgcgg cggacatcga aaaagcgatt aacgattttg ccgaagcagc aaaacgagcc
      961 gtcaacgcgg gttttgaagt cgtcgaaatc cacgccgcgc acggttatct gctgcacgaa
     1021 tttctttcgc cgatttccaa tcaaagaacg gacgaatacg gcggaagctt tgaaaatcgc
     1081 gctcgctttc cgcttgaaat atcgagaaaa gttcgcgccg ccgtgccgga aaatctgccg
     1141 cttttcgtga gaatttcggc gaccgactgg acggaaggcg gttgggattt ggagcagtca
     1201 atcgagtttt gccgaaaatt aaaagaaatc ggcgttgacc tgattgactg ctcgacgggg
     1261 ggaaacttac cgcaggcgaa aattcccgtc gcgccgaatt atcaagtgcc gtttgccgcc
     1321 gaaattcgcc ggaaagccgg cattttaacg gcggcggtcg ggatgattgc cgaaccgcag
     1381 caggctgaag aaattttgcg gaaaggcgag gcggacgccg tgatgattgc gcgccagttt
     1441 ctgcgcgagc cttattttcc gtttcgcgcg gcgcgtgagc tgggcgcaga gattgacgtt
     1501 ccgaaacaat acgggcgggc gattgaaatt caaaccgcca aaggagcgaa gagataaacg
     1561 atgaacgcga aacgatgaac gatgaatttg aaacgtcgaa actgcaattc atcgttcatc
     1621 gttcctgatt catcgtttct ttaaggggaa ttatgagcag cttttcaaac attccgtttt
     1681 cggttttgga tttagcgccc gttgtgaaag gcggcagcgt cggcgacgct ctgcgcgatt
     1741 cgctttcgct ggcgcagcac gcggaaaagc tcggctacag gcgtttctgg cttgccgaac
     1801 atcacaatat gacgggtatt gcgagcgcgg cgacctcggt cgtcatcggc tacgtggcgg
     1861 gcgggacatc gaaaatccgc gtcggcgcgg gcggaattat gctgccgaat cacgcgccaa
     1921 tcgttatcgc cgaacaattc ggaacgctcg aaacgcttta tccgaaccgc atcgatctgg
     1981 gcttgggacg cgcgcccgga agcgaccagg caacggcgct cgccctgcgc cgcacgctgc
     2041 acacgagcgg cgacgatttt cccgaactgc tggaagaact ccggtttttt ctgcgcgagc
     2101 cggtcgcggg tcaaagagtc agagccgtgc cgggcgcggg cttacacatc ccgatttggc
     2161 tgctcggttc gagcggtttc agcgcgcggc tggcgggcga gctgggtttg ccgttcgctt
     2221 tcgcgggaca tttttcgccg gaatatattc tgcccgcgct ggagctttat cgaaattctt
     2281 tcgagcctgc gggcgcgctc gaaaagccgc tggcgatgct ggcgataaac gccgtcgccg
     2341 ccgatacgga cgaggaagcg tggcgactgg caagctcgca gtttcagtcg tttttgaatt
     2401 tgattcgagg caagcccgga cagcttcagc cgccggtcga aaatatggac gagctttgga
     2461 cgccgcagga aaaagcggcg gtcgaatcaa agctcggcgg ctcgataatc ggcagcgcgc
     2521 cgaccgttaa agagaaactg gaaaagattt tggcggaaac gggcgccgac gaattgatga
     2581 taaacgcgat gatttacgac cacgcggcgc ggctgcgctc gtatgaaatc gtcgccgacg
     2641 tctggcagga taaaacggag agatattatg tgaaaaagtg aaaaagtgaa agtgtgaaaa
     2701 ggtgaaaaag ttttcttttg caaaactaca agtgcttttt cactttta
//
LOCUS       DASWNE010000474         2675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_612519, whole genome shotgun sequence.
ACCESSION   DASWNE010000474 DASWNE010000000
VERSION     DASWNE010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612519"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..1574
                     /locus_tag="VGB68_09410"
     CDS             12..1574
                     /locus_tag="VGB68_09410"
                     /inference="COORDINATES: protein motif:HMM:NF039502.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5691 domain-containing protein"
                     /protein_id="HEY0049489.1"
                     /translation="MKLWREIVSATIVGTERQRFELSADVDNQLSRVLRSLKSDNNET
                     ALLKSAAVTGVYLKNGVNSAADKEDSPVAAPDEELAFISRQSEQYLAAMLAGNHPKIL
                     FEFFDLAAEKKRVLPPEFLAEALDLGSSKRDLRKFILPALGARGRWLARYNNDWRWAL
                     IEENAVEIWENGSRDERLLAIEVLRRQNPAEIGELLSRDWQSESAKDRAAFLEVLRIN
                     LNKSDESFLDKILQTDKSIDVRRIAFDLLLAITDSNLVEIITQRAAALFNFKTGGFLS
                     KPKIEINFPENFEKESKDDVLNNLELYPGEKDLGKKAVKIVKLLGSVPPKIWEEKFST
                     KKEIIIEAAKQSDWQTALLGGWKSAAVNFADQQWLVSILTNNGQNDYYLLCGQIERRW
                     TGLQIERLVALLLEGKMKSGIAPEFAAQLLYRLKNIWSDDFTLGVLNFLKKSESESDS
                     RQWSGFITQILNSGQFIAGKYLKQFEEKASSGLFERLCQLPEKEKSIEEFIAVIQFRR
                     EMHTAFQSEEQL"
     gene            1571..2650
                     /locus_tag="VGB68_09415"
     CDS             1571..2650
                     /locus_tag="VGB68_09415"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0049490.1"
                     /translation="MINVLREHAEQQFAEELNELAKEDKKQRPPNWKLSPWSVATYLL
                     GGKTESGLEITPKYIGSRRLMEIAIATLATDRALLLLGVPGTAKSWVSEHLAAAVTGD
                     STLIIQGTAGTGEEAIRYGWNYAKLLAEGPSLGALVASPVKRAMEDGKIARVEELTRV
                     PADVQDTLITILSEKTLPIPELNTEIQAVKGFNIIATANDRDKGVNELSSALKRRFNT
                     VVLPVPATIEEEVEIVERRVASLGKSLELPAEIPALSEIRRVVQIFRELRNGVTSDGK
                     TKLKSPSGTLSTAEAISVINNGLSLAAYFGDGVLNAQDLASSVVGAVIKDPVQDRVVW
                     LEYLETVVKERDGWKDIYRACREKI"
BASE COUNT          717 a          633 c          732 g          593 t
ORIGIN      
        1 ggttataaat tatgaagttg tggcgggaaa tagtttcggc gacgattgtc ggcactgaac
       61 ggcaaagatt cgaattgtcg gcggacgtcg ataatcagct gagccgcgtt ttgcggtcgc
      121 tgaaatcaga taacaatgag accgccttgc tcaagtctgc ggcggtaacc ggcgtttatc
      181 tcaaaaacgg cgtcaacagc gcggcggata aagaagattc gccggttgcc gcgccggacg
      241 aagaactcgc ttttatttcg cggcagtccg aacaatatct ggcggcgatg ctggcgggca
      301 accacccgaa aattttattc gaattttttg atttagcggc agaaaaaaag cgcgttttgc
      361 cgcccgaatt tttggcggaa gctctcgatt tgggcagttc gaaacgtgat ttgcgaaaat
      421 ttattttgcc ggcgctgggc gcgcgcgggc gctggctagc gcgttataat aacgattggc
      481 gttgggctct gattgaagaa aacgccgtcg agatttggga aaacggctcg cgcgacgagc
      541 ggcttctggc gattgaagtg ctgcgtcggc aaaatccagc cgaaattggc gagcttttga
      601 gccgagattg gcagagcgag tccgctaaag accgggcggc gtttctcgaa gttttacgga
      661 taaatctaaa taaatccgat gaatcctttt tggataaaat tctgcaaacc gataaaagca
      721 tcgacgttcg gcgcatagcg ttcgatttat tgctcgcaat tacggattcg aatctggttg
      781 aaattatcac gcagcgcgcc gccgcgctct tcaattttaa aacgggcggt tttctttcaa
      841 aaccgaaaat cgaaatcaat tttcccgaaa actttgaaaa agaatcgaag gacgacgtgc
      901 tgaacaatct cgagctttat cccggcgaaa aagatttagg aaaaaaggcg gtcaaaatcg
      961 ttaaattgct cggctcggtt ccgccgaaaa tctgggaaga aaagttttcg acaaaaaaag
     1021 aaatcatcat cgaagcggca aagcagagcg actggcaaac ggctctgctc ggcgggtgga
     1081 aatccgccgc agtcaatttt gccgaccagc aatggctggt ttcgatttta actaataacg
     1141 gacaaaatga ttattatctg ctttgcgggc aaatcgagcg ccgctggacg ggtttgcaaa
     1201 tcgaaagact cgtcgcgctt ttgcttgaag gcaaaatgaa aagcggaatc gccccggagt
     1261 ttgccgcgca gctgctctat cgtctgaaaa atatctggag cgatgatttc acgctcggag
     1321 tgctgaattt tctgaagaaa tcggaaagcg aaagcgactc gcggcagtgg agcggattta
     1381 tcacacaaat cttaaattcg ggacaattta tcgccggaaa atatttaaag caatttgagg
     1441 aaaaagcgtc ttccggactg tttgaaaggc tttgccagct gccggaaaaa gaaaaatcaa
     1501 tcgaggaatt tatcgccgtc atacaatttc gccgcgagat gcacacggct tttcaaagtg
     1561 aggaacaatt atgattaatg ttttaagaga acacgccgaa cagcaattcg ccgaagagct
     1621 gaatgagctc gccaaagaag ataaaaaaca gcgtccgccg aactggaagc tctcgccctg
     1681 gtcggtggcg acttatctgc tgggcggaaa aacggaaagc ggattggaaa tcacgcccaa
     1741 atatatcggc agccgccgtt tgatggaaat cgccatcgcc actctggcga ccgaccgcgc
     1801 gcttcttctg ctcggcgttc ccggcacggc gaaatcctgg gtttccgagc atctcgccgc
     1861 cgccgtcacg ggcgattcga ctttaattat tcaaggcacc gccggcaccg gcgaagaagc
     1921 gattcgctac ggctggaatt acgccaaatt gctcgccgaa ggtccgagcc tgggcgcatt
     1981 ggtcgccagt ccggtcaaac gggcgatgga agacgggaaa atcgcccgcg tcgaagagct
     2041 gacgcgcgtt ccggcagacg tgcaggatac gctgattacg attctttcgg aaaagacttt
     2101 gccgattccc gaacttaaca cggaaattca ggcagttaaa ggatttaaca taatcgccac
     2161 ggcaaacgac cgcgataaag gagtcaacga attatccagc gcgctgaaac gccggtttaa
     2221 tacggtcgtt ctgcccgtcc cggcaacgat tgaagaagaa gtcgaaatcg tcgagcgccg
     2281 cgtggcaagc ctcggaaaat cgctggaatt gcccgccgaa attccggctt tgagcgaaat
     2341 ccgccgcgtc gtgcaaatct ttcgcgagct ccgaaacggc gtcacgtccg acggaaaaac
     2401 caagttgaaa agtccgagcg ggactttgag cacggcggaa gccatttcgg ttatcaacaa
     2461 cggtttgtct ctcgccgctt atttcgggga cggagttctg aacgcgcagg atttggcttc
     2521 gtcggtagtc ggcgcggtaa taaaagaccc cgtgcaggat cgcgtcgttt ggctggaata
     2581 tctggaaacg gtcgttaagg agcgcgacgg ctggaaggat atttaccgcg cctgccgcga
     2641 aaaaatttaa atgaagctga caattttcgg aatca
//
LOCUS       DASWNE010000475         7788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_157291, whole genome shotgun sequence.
ACCESSION   DASWNE010000475 DASWNE010000000
VERSION     DASWNE010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7788
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157291"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VGB68_09420"
     CDS             <1..259
                     /locus_tag="VGB68_09420"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY0049491.1"
                     /translation="SGRVTSQSTGDGLSGVTVILSGGGLTTPRIARTNPFGYYTFDGV
                     PQNQTYTVTVASKIHQFASPSQMVSLATTNVTNVNFVGTTP"
     gene            complement(346..1008)
                     /locus_tag="VGB68_09425"
     CDS             complement(346..1008)
                     /locus_tag="VGB68_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049492.1"
                     /translation="MTAGQTRRYEMFLKVRDFFAAHASDFPAASIAATIFAPLIGIID
                     QLAQIAGEQISTRGTYSQTIEVKGDARDILFVMLQDISDLATTLAYEINGLEEKFRLP
                     RNRRDQNLIAAGRAFAADAAAFEDEFIGYGLSPTFIADLTAATDAFEQSLIPANIAGQ
                     EKTGKTAAAAPLVDAGMIIVRRLTPVVRKKYRNDAGNLAAWEFARHVERTQPKTAPPP
                     NP"
     gene            complement(1544..1897)
                     /locus_tag="VGB68_09430"
     CDS             complement(1544..1897)
                     /locus_tag="VGB68_09430"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HEY0049493.1"
                     /translation="MIRFLADEDFDNRILRGLLRRLPQLDIMRVQDVGLQSAHDTEVL
                     AFAAETNRVLLTHDVRTMLECANQRIAKGLPMPGVFGIPQSMPIGEAIEDLILIAEYS
                     LENEWQEQIRFLPLH"
     gene            complement(1894..2226)
                     /locus_tag="VGB68_09435"
     CDS             complement(1894..2226)
                     /locus_tag="VGB68_09435"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEY0049494.1"
                     /translation="METVLNVSQNVLRRDESGVWRVGESRVSLDSVIYAFHEGASAEE
                     IALRFPTLELVQIYSVISYYLQNREEIDNYLAAQQKQHDELKHQIESRFSPAGIRQRL
                     LERAQNKK"
     gene            2437..3276
                     /locus_tag="VGB68_09440"
     CDS             2437..3276
                     /locus_tag="VGB68_09440"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0049495.1"
                     /translation="MNIIQKIKGWFPFLNKKAEHSEEDNNFDFSAQNPKIGKNELETL
                     GILASSIEHDVKNPLNVIQAEIYHMGRRFQHNEEVLNFLNRIEEQTQRIYAALQVIPL
                     FRGEKFYEQNLKKVSLNEIVWKSISDVRRGMIDDSNIFFKKGGYLNEGNLKVKAYAPL
                     LQQAIINVLKNAIEAIHETKRKNGVIYINVRKNRVSSEKFVIIEITDNGCGMAEVDVE
                     KLTTLYTTKDHKKPNSGLGLFFTKRILSIHSGEIRIESEFGKGTTVSLILPELLPELK
                     HRH"
     gene            3298..4137
                     /locus_tag="VGB68_09445"
     CDS             3298..4137
                     /locus_tag="VGB68_09445"
                     /inference="COORDINATES: protein motif:HMM:NF021640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061810 - NAD glycohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEY0049496.1"
                     /translation="MNKKKILLVDDDPFFVERMKAILIDEGLEVITSENVSSAKQKIK
                     EHNDTTFILLDIRMPSGEDSDLETQMGYKSGVVLARWVKQNYPQINIVGLSADDSLDV
                     KEWFRQHSIPLFEKGTGALQDILNILLNKNKHKQIRTFIAHGHDDAAKYQLKNYLQNT
                     LHLPEPIILHEQASLGRTIIEKFEEFANQIDVVFVLLTPDDIASGSLDALKKRARQNV
                     IFEMGYFFSKLQRRKGKVILLYKGQLEPPSDISGIIYIDISNGIEAAGEQIRKELSSL
                     LER"
     gene            complement(4230..5882)
                     /gene="groL"
                     /locus_tag="VGB68_09450"
     CDS             complement(4230..5882)
                     /gene="groL"
                     /locus_tag="VGB68_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY0049497.1"
                     /translation="MAKQVIHGEDSRAAILRGVNQLADAVKVTLGPKGRNVVIDKKFG
                     SPTITKDGVTVAKEIELKDSLENMGAQMVREVASKTSDVAGDGTTTATVLAQAIFKEG
                     VRTVAAGANPMALKRGIDKAVELIVAEVQRLAKPVSGDSIAQVGTVSANGDKTIGNII
                     AEAMDKVGKDGVITVEEAKTMDTSLEVVEGMQFDRGYLSPYFVTDADRMEAVLDNPLI
                     LINEKKISSMKDLLPILEQVAKMGKPLLIIAEDVEGEALATLVVNKLRGTLNVAAVKA
                     PGFGDRRKAMLEDIATLTGGQVISEDLGIKLESITADDLGKAKKVTIDKDNTTIVEGA
                     GAGEAIEGRVKTIRNQIEETSSDYDREKLQERLAKLVGGVAVIKVGAATETEMKEKKA
                     RVEDAMHATRAAVEEGIVPGGGVTLVRASKVLDSYQVEEGGDTDEQIGVNIIKRAVEE
                     PLRQIAGNAGKEGAVIVEKIRAEDNETFGFNAQTEVFEDLVAAGVIDPAKVTRTALQN
                     AASIAGLMLTTEAMIADIQDEKGEGGMGGGMPGGMGGMGMGM"
     gene            complement(6010..6330)
                     /locus_tag="VGB68_09455"
     CDS             complement(6010..6330)
                     /locus_tag="VGB68_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEY0049498.1"
                     /translation="MATTIKPLHDRVIVRRLDESSNQTAGGLFIPDSAKEKPQEGEVI
                     AAGAGKYKEDGSRQTLDVKEGDRVLFGKYSGSEIKIDGEELLIMREDEILGIIERANA
                     GNAA"
     gene            6524..7357
                     /locus_tag="VGB68_09460"
     CDS             6524..7357
                     /locus_tag="VGB68_09460"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HEY0049499.1"
                     /translation="MILSEINIYPIKSLKGISLEETKIERRGLQFDRRWMLVDEKNKF
                     FTQREFPKMATIDVQIGENGLEVSENGSKLEIPFSPNNNETARVAVWSSKCAAKIYEK
                     RVNEWFSDVLQTNCRLALMLEETERKVNYFYAVHRDDHVSFADGYPFLLIGENSLADL
                     NSRLEDDLPMNRFRPNFVVKDSEPFAEDGWKKIKIGASLFHVVKPCGRCVMTTIDQST
                     GEKDGKEPLKTLASFRIPKRSVKKKILFGQNLIVENAGDILRVGDKIEVLETKNKPRF
                     V"
     gene            complement(7359..7607)
                     /locus_tag="VGB68_09465"
     CDS             complement(7359..7607)
                     /locus_tag="VGB68_09465"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0049500.1"
                     /translation="MQIRTRMESLIEEMLDGQIMLCEALSEFEKLYIQKALDRYDEHL
                     SKTANALGIHRNTLSKRVATYQTPAKTPKRLLTARRTK"
BASE COUNT         2052 a         1798 c         1803 g         2135 t
ORIGIN      
        1 cagcggaaga gtaacatcgc aatcgacggg cgacggctta tcgggcgtga cggtcattct
       61 gtcgggcggc ggtctgacga ctccgcgcat cgcgcgcacc aatccgttcg gttattacac
      121 tttcgacggc gttccgcaga atcaaaccta cactgtgacg gtcgcgtcta aaattcatca
      181 attcgcgagt ccgagtcaga tggtttcgct ggcgactaca aatgtgacaa acgtcaattt
      241 tgtcggcacc acgccctgac gagtattcaa gcggaagttc tccaaatgac aaaaggcttc
      301 gctcaatacc gggcgaagac ttttatcatt cggaaatttg ctcttttaag gattcggcgg
      361 cggcgcggtt ttcggctgcg ttcgctcgac gtgccgggca aactcccaag ccgccagatt
      421 accggcgtcg ttacgatatt tttttcgcac caccggcgtc aaacgcctga cgataatcat
      481 tcccgcatcg accagcggcg cggcggcggc agtcttgccc gttttctcct gcccggcgat
      541 atttgccgga atcaaagact gctcgaaagc gtcggtcgcc gccgtcaaat cggcgataaa
      601 ggtcgggctc aaaccgtagc cgataaactc gtcttcaaac gccgccgcat cagcggcaaa
      661 agcccgcccg gcggcaatca gattctggtc gcgccggttg cgcggcaggc gaaacttttc
      721 ttccagcccg tttatttcgt aagccagcgt cgtcgccaaa tccgatatgt cttgcagcat
      781 aacaaacaga atatcgcgtg catcgccctt aacctctatc gtctgcgaat aagtgccgcg
      841 cgtcgaaatc tgttcgcccg caatctgcgc gagttggtcg ataatcccaa tcaaaggcgc
      901 aaaaatcgtc gccgcaatcg aagcggcggg aaaatccgac gcgtgcgccg caaaaaaatc
      961 acgcactttc aaaaacatct cataacgccg tgtttgacca gcagtcataa atttaatctc
     1021 cttatttata ttggatttgt ctaccgacgg cagcaggctg tgggcagagt tgtttaacaa
     1081 gaattttcct gcaagatgac actttcaccc gcaagatgac gcttttaacc gcaagacgac
     1141 actttcacgc gcaagattga actttcagcc gcaatattaa acttttacgc gcaagataac
     1201 acttttgccc gcaatatcgc tatttcaccc gcaagatgga acttttaccc gcaatctcaa
     1261 actttcacgc gcaagataca tctttcacta aattttcttt taaaaagctg agcatcgaga
     1321 cacaaaaaag ccattcccgt gccagaactt tacgcccgcg ccgaaaatcc gcgaagtatt
     1381 gagatagtta ggaaaactta attgtttatt gattattgcg ggagacgcgc aaggtcggaa
     1441 ataatatcaa ttctgatagg cgaacaatca tttttgatag tattacgcag gcggaaacgc
     1501 taaggagcgc gtaacttttt agtttggggc gacggattga ttattaatgc aagggtagaa
     1561 atctgatttg ttcctgccat tcgttttcca gactatattc agctatcaag attaaatctt
     1621 caatcgcttc tccgattggc atactttgag gaattccgaa aacgcccggc atcggcaaac
     1681 ctttagctat tctctgattg gcgcattcga gcatcgttct gacatcgtgg gttaaaagca
     1741 cgcggtttgt ttcagcggca aatgctaaaa cttccgtatc atgcgccgac tgcaagccga
     1801 catcctgaac gcgcattata tcaagttgcg gcaaacgacg cagaagcccg cgcaaaattc
     1861 gattatcgaa atcttcgtca gccagaaaac gaatcatttt ttattttgag cgcgttcgag
     1921 aagcctttgc ctaatgcctg ccggcgaaaa acggctttcg atttgatgtt ttaattcgtc
     1981 gtgttgtttc tgttgcgcgg cgagataatt atctatttct tcacgatttt gcaggtaata
     2041 actaataacc gaataaatct gcaccagttc gagcgtcgga aaacgcaaag cgatttcttc
     2101 agcgctcgcg ccctcatgaa aagcataaat caccgaatcc agcgaaaccc gactttcccc
     2161 gacgcgccaa accccgcttt catcccggcg caaaacattc tgcgagacat tcaaaacggt
     2221 ttccatttcc tgctcacctc gccttcgatt ttaacacaat agcgatattg cctctaaagc
     2281 aaagaatttt ttatttttgc aagaaatttt gttaacatac ctgccacaaa acgcggaaag
     2341 cactttgaag gcttggaagt aacattcggc tagtattaat tattaggcat tatcgaactc
     2401 tcaaaattgt tgaaatgatt tttgcaagta ttttatatga acataattca gaagataaaa
     2461 ggttggtttc ctttccttaa caagaaagca gaacattccg aggaggataa taatttcgat
     2521 ttttcggctc aaaatcctaa aattgggaaa aacgagttgg aaacattagg aatattggca
     2581 tctagtatag aacacgacgt taagaaccct ctaaatgtta ttcaagctga aatttatcat
     2641 atggggagga gatttcaaca taatgaggag gtattaaatt ttttaaatag aatagaggaa
     2701 cagacgcaaa gaatttatgc tgctctgcaa gttatacctt tatttcgcgg ggaaaaattt
     2761 tacgagcaaa atttaaagaa agtatctctt aacgaaattg tatggaaaag catttcggat
     2821 gtccggcgcg gaatgataga tgattctaat atttttttta aaaagggtgg ttaccttaat
     2881 gaaggtaatt taaaggtaaa agcatatgct ccgcttttac aacaggcaat tattaatgtc
     2941 ttaaaaaatg cgatagaagc tattcacgaa acgaaacgta aaaatggagt tatctatata
     3001 aatgtccgta agaatagagt tagtagtgaa aaattcgtaa ttattgaaat aaccgacaat
     3061 ggatgcggta tggcagaagt ggacgtcgag aaactaacta ccttatacac gactaaagac
     3121 cacaaaaaac ctaacagcgg gttaggtttg ttctttacca agagaattct ttccattcat
     3181 agcggggaaa ttagaatcga aagtgaattc ggaaaaggaa caacagtctc tctaatactt
     3241 cctgaactgc ttcccgaact taaacacagg cattaatttt tatatttcaa ttggcatatg
     3301 aacaaaaaga aaattctttt agtagatgac gacccttttt ttgttgagcg aatgaaggca
     3361 atacttatag acgaaggttt agaggttata acatctgaga atgtaagtag tgctaaacag
     3421 aaaataaagg agcacaatga tactactttt attcttttgg atataagaat gccgtcgggc
     3481 gaagattctg accttgaaac acagatgggc tataagtctg gagtcgtatt agcgcgctgg
     3541 gtaaaacaaa attaccctca aataaatatt gtaggtttgt cggctgacga ctcgctggat
     3601 gttaaagaat ggttcagaca acactcaatc cctctctttg aaaagggaac aggagcgctt
     3661 caggatatat tgaatatact attaaacaag aataaacata aacagattag gactttcatt
     3721 gcacatgggc acgacgatgc cgcaaaatat cagcttaaaa attaccttca aaatactttg
     3781 catttgccgg agcctataat tcttcatgaa caagcttcac ttggtcgaac tatcatcgaa
     3841 aagtttgaag aattcgcaaa tcaaattgat gtagtattcg ttcttttaac accagatgat
     3901 attgcttctg gttcgcttga cgctttaaaa aaacgcgcta gacagaatgt aatttttgaa
     3961 atggggtatt tctttagtaa gttacaaaga agaaagggga aagttatact tctttataaa
     4021 ggtcaattgg agccgccttc tgatatttct ggaattattt atattgatat ttctaacgga
     4081 atagaagcag cgggtgaaca aatacgaaag gagctttcat ctcttctaga aagataatac
     4141 ttttattata atcagtataa aaaaggcttc gttcattcaa gagcgaagcc ttttttacct
     4201 ttcgttgttt ataactgctt gagagtagat tacattccca ttcccatgcc gcccatgccg
     4261 ccgggcattc cgccgcccat gccgccttcg cctttttcgt cctggatgtc ggcgatcatc
     4321 gcttcggtgg tcagcatcaa gccggcaatc gaagccgcgt tttgcagagc cgtgcgggtg
     4381 actttcgccg ggtcgatgac gcctgccgcg acgaggtctt cgaagacttc ggtctgcgcg
     4441 ttaaagccga aggtttcgtt gtcttcagcg cggatttttt cgacgatgac cgcgccttcc
     4501 ttaccggcgt tgccggcaat ctggcgaagc ggttcttcga cggcgcgctt gatgatgttg
     4561 acgccgattt gctcgtcggt gtcgccgcct tcttcgacct gatacgaatc gaggactttc
     4621 gaagcgcgaa ccagtgtcac gccgccgccc gggacgattc cttcttcgac cgccgcgcgc
     4681 gtagcgtgca tcgcatcttc gacgcgagct tttttctctt tcatctcggt ttcggttgcc
     4741 gcgccgactt tgatgacggc gacgccgccg accagtttgg cgagacgctc ctgcaatttt
     4801 tcgcggtcgt aatccgaaga agtttcttcg atttgattgc ggatggtttt cacgcgacct
     4861 tcgatggctt cgcccgcgcc cgcgccttcg acaatcgtcg tgttgtcttt gtcaatcgtg
     4921 actttcttgg ctttgccgag gtcgtcagcc gtgatgcttt cgagcttgat gcccaaatct
     4981 tcgctgataa cctgtccgcc cgtcagggtc gcgatgtctt cgagcatcgc tttgcggcgg
     5041 tcgccgaagc ccggcgcttt gacagccgcg acgttcagcg ttccgcgcag tttattgacg
     5101 accagggtcg ccagcgcttc gccttcgacg tcttcggcga taatcagaag cggtttgccc
     5161 atcttggcaa cctgctcgag aatcggcaga aggtctttca tcgaagaaat tttcttttcg
     5221 ttgataagaa tcagagggtt gtcgagaacc gcttccatac ggtcggcgtc ggtcacaaaa
     5281 tacggcgaaa gatagccgcg gtcaaactgc ataccttcga cgacttccag cgaggtgtcc
     5341 atcgttttgg cttcttcaac cgtgataacg ccgtctttgc cgactttatc catcgcttcg
     5401 gcgataatgt tgccgatggt tttgtcgccg ttggcggaaa ccgttccgac ctgcgcgatt
     5461 gaatcgcccg agacgggttt ggcaagacgc tgcacttcgg caacaatcag ctcaaccgct
     5521 ttgtcgatgc cgcgctttaa agccatcggg ttggctccgg cggcgacggt gcgcacgcct
     5581 tctttgaaaa tcgcctgcgc cagaacggtc gcggtcgtcg tgccgtcacc ggcaacgtcc
     5641 gaggttttcg aggcgacttc gcgcaccatc tgcgcgccca tattttccaa agaatccttt
     5701 aattcgattt ctttcgccac ggtcacgccg tctttggtga tggtcggcga gccgaatttt
     5761 ttgtcaatca cgacgttgcg acctttcggt ccgagcgtca ctttcactgc gtcggcgagt
     5821 tgattgacgc cgcgcaggat agctgcgcgt gaatcttctc catgaataac ttgtttagcc
     5881 atagttttat aaatctcctg ttaaaaattt ttaagttgac tgagttgact gaaagttgac
     5941 cgagtttgcc gagttatccg agcgtttctt ataaactcgg tcaactcgca actcgctgca
     6001 ctcagcgaac taagcggcgt tgcccgcgtt ggcgcgttcg atgattccga gaatttcgtc
     6061 ttcgcgcata atcaaaagct cttcgccgtc aattttgatt tccgagccgc tgtatttgcc
     6121 gaacaaaacg cggtcgcctt ctttgacgtc aagcgtttgg cgcgagccgt cttctttata
     6181 tttgcccgcg ccggcggcga ttacttcgcc ttcctgcggt ttttctttag ccgaatcggg
     6241 aatgaagagc ccgcccgctg tctgattgct tgattcgtcg agccgacgca cgataacacg
     6301 gtcgtgtaac ggtttaattg ttgtagccat ttatattttc tcctttcggg atttttgaaa
     6361 aagttttatt attaaattgg aacgacggcg cgaaatttat ttgttagcac tgtccttcca
     6421 agagtgctag tataaaaaaa cagagttaaa cctgtcaagt gcggttattt aatttaactt
     6481 gattttttag aaaatctgat aatgccgtta tcaaatttat tctatgattc tatccgaaat
     6541 taacatttat ccgattaaat ctttaaaggg tatttcgctc gaagaaacta aaatcgagcg
     6601 gcgcggtctg caattcgacc ggcgctggat gctggtcgac gaaaaaaata aatttttcac
     6661 gcagcgcgag tttccgaaaa tggcgacgat tgacgtgcaa atcggcgaaa acggtttaga
     6721 ggtttcggaa aatggaagca aactggaaat tcctttttcg ccgaacaata acgagacggc
     6781 gcgcgtcgcc gtctggagca gcaaatgcgc ggcgaaaatt tatgaaaagc gggtcaacga
     6841 atggttcagc gacgttttgc agacaaattg caggctggct ctgatgctgg aagagaccga
     6901 gcgcaaagtc aattattttt acgccgtgca cagagacgac cacgtttcgt ttgccgacgg
     6961 ttatccgttt cttttaatcg gcgaaaattc gctggctgat ttaaattcgc ggcttgaaga
     7021 cgatttgccg atgaatcgct ttcgtccgaa ttttgtcgtc aaagattccg agccttttgc
     7081 cgaagacggc tggaaaaaaa tcaaaatcgg cgcgagcctt tttcacgtag ttaagccgtg
     7141 cgggcggtgc gtgatgacga cgattgacca gagtaccggc gaaaaagacg gcaaagagcc
     7201 tctgaaaact ctggcgagct ttcgcatacc gaaaagaagc gtcaagaaaa aaattctttt
     7261 cgggcagaat ttaattgtcg aaaatgctgg agatattttg cgagtcggtg acaaaatcga
     7321 agttctggaa acgaaaaaca agccgagatt tgtatgaatt atttagttcg ccgcgcggtc
     7381 aggagccgtt tcggagtttt cgccggagtt tggtaagtcg cgacgcgttt cgagagcgtg
     7441 ttgcgatgaa tgccgagcgc attggcggtt ttggaaagat gctcgtcgta gcggtcgagt
     7501 gccttttgaa tatagagctt ttcaaattcc gaaagcgcct cacagagcat aatctgcccg
     7561 tccagcattt cttcaatcaa cgattccatt cgcgttctta tttgcataat tcaattcaat
     7621 tacgaattac aaattacgaa ttacgaatga aggaaatttt cttttaattc gtaatttgta
     7681 atttgtaatt cgtaattgtc aaagttcttt ccccgttaaa attttatagg cttccagata
     7741 gcgtgcggtg gtcgcttcga cgatttccgg cggaagcggc ggcgcggg
//
LOCUS       DASWNE010000476         3280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_612549, whole genome shotgun sequence.
ACCESSION   DASWNE010000476 DASWNE010000000
VERSION     DASWNE010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612549"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VGB68_09470"
     CDS             complement(<1..361)
                     /locus_tag="VGB68_09470"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY0049501.1"
                     /translation="MNNAQFGMVGLGTMGRNFLLNVAEHGFSCAGFDIDEAKRQLLLA
                     ESGGFEVAAAGSLIEFIAALERPRRIMLLVPAGKIVDAVINDLLPHLETGDIIIDGGN
                     SHFLETERREILLGEKGI"
     gene            complement(370..1284)
                     /locus_tag="VGB68_09475"
     CDS             complement(370..1284)
                     /locus_tag="VGB68_09475"
                     /inference="COORDINATES: protein motif:HMM:NF016533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEY0049502.1"
                     /translation="MKQAIWANLPQKFTELISGSFREEGKRWIADLPRILKEIEQNWS
                     VEIKKPFPNLSYHYVAPCVFKGGGEAVIKIGLPGEKQTTFNEIRMLEFLDGKSVCKIL
                     RFDEKRFALLLEKLTPGENLKTICRNDDAEAVEIAVKVMRDFWRQAPENIEFPKLEKW
                     FEDLQAAEKIGFAPEYVKKARRFFDELMTASAQKTLLHGDFHHENILSAEREPFLAID
                     PKGVVGDIGYDLSVFMINHAGWLKSEPNLPEKLDDAIRAFSVAFEIEPRALRKWIFAH
                     SVLSAWWTFEETSENWKNELALAEIWKV"
     gene            1322..2230
                     /locus_tag="VGB68_09480"
     CDS             1322..2230
                     /locus_tag="VGB68_09480"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY0049503.1"
                     /translation="MKSLHEMIRPTTFVEPEKLKKHLDIDLTIAAETFQHTGSFKFRA
                     AYNLASKVANNEILTASSGNFGQALALACQLVGKKCTVVMPETSARVKIEAVRSYGAA
                     VDLVDTNKIGRNERVAQLAAEMPDAYFASAYDDLFVIEGNSTLGDELSKKDFDVVVSP
                     VGGGGLISGISKGLRRNSHRAVLIGAEPLLGNDAFRSLKAGEIVRNDVEPLSIADGAR
                     TISLGKHNWEIIKTDVAEIIEVSEEKIIEAVRLYFSLANLKVEPTGALSLGAILESGE
                     KFKDKKVCLVVSGGNVDTEIYRQIIA"
     gene            complement(2423..3103)
                     /locus_tag="VGB68_09485"
     CDS             complement(2423..3103)
                     /locus_tag="VGB68_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049504.1"
                     /translation="MKLFILSTAVLLLFTIQTFAQPEGNPENLCRNGFFPRESSRYRL
                     ASVKGSKNEKVHFYGDDRNCPNDKNCQTKSYVIPGDEVIVSRRFGKWACGWYQPKKGW
                     ETVGWLALDKLEFKETNPEPSINDWLGNWNYNNGSQIEIGKSKTAGLLTITGSAFWKG
                     LGDNIHTGELDDQSKPAGNELKIGESETGEYDCKVAMRLLGKYLIVTDNLQCGGLNVT
                     FSGVYQKK"
BASE COUNT          785 a          859 c          767 g          869 t
ORIGIN      
        1 cgattccctt ttcgcccagc agaatttcgc gccgctcggt ttcgagaaag tgcgagtttc
       61 cgccgtcgat gatgatgtcg cccgtttcca ggtgcggcag caaatcattg atgaccgcgt
      121 cgacgatttt gccagccgga accagcagca ttatccgtcg cggtcgttcc agcgcggcga
      181 taaattcgat gaggctgccc gccgccgcga cttcaaagcc gccggattcg gcgagcagca
      241 attgacgctt tgcctcgtcg atatcaaaac cggcgcacga aaaaccgtgt tccgcgacgt
      301 tcagcagaaa atttctgccc atcgttccaa gtccgaccat tccgaattgt gcgttgttca
      361 taaaattaat tatactttcc agatttccgc taaagccaac tcatttttcc aattttccga
      421 agtttcttca aacgtccacc acgccgacaa aaccgaatgc gcgaaaatcc attttcgcaa
      481 agcgcgcggc tcgatttcaa aggcgacgga aaacgctcga atcgcatcgt ctaatttctc
      541 cggcagattc ggctccgact tcagccagcc ggcgtgattt atcataaaaa cgctcaagtc
      601 gtagccgatg tcgccgacca ctcctttcgg gtcaatcgcc agaaaaggct cgcgctccgc
      661 cgataaaata ttttcgtgat gaaaatcgcc gtgcaaaagc gttttttgcg cggacgcggt
      721 cattaattca tcaaaaaacc ggcgcgcttt ttttacgtat tcgggagcaa aaccgatttt
      781 ttcagccgcc tgcaaatctt caaaccattt ttccagcttc ggaaactcaa tattttccgg
      841 cgcttgccgc cagaaatcgc gcatcacttt gaccgcaatc tcaaccgctt cggcatcgtc
      901 attgcggcaa atcgttttta gattttcgcc cggcgtgagt ttttccagca gcaaggcaaa
      961 acgtttttcg tcgaatcgca aaattttgca gacgcttttg ccgtcgagaa attcgagcat
     1021 tcttatttca ttaaaggtcg tctgtttttc gccgggcaag ccgattttga tgaccgcttc
     1081 gccgccgcct ttgaaaacgc acggcgcgac gtaatgatac gacaaattcg gaaacggctt
     1141 ttttatttcg accgaccagt tttgctcgat ttccttgaga atccgcggca aatcggcaat
     1201 ccagcgcttt ccttcctcgc ggaatgagcc cgaaattaat tcggtaaatt tctgcggtaa
     1261 attcgcccaa atagcttgtt tcattcttga cgagtttaac tatcttgtag attctaaaca
     1321 aatgaaatct ttacacgaaa tgattcgccc gaccactttc gtcgagccgg aaaaattaaa
     1381 aaagcatctc gacatcgatt taaccatcgc cgccgaaact ttccagcaca ccgggagctt
     1441 taaattccgc gccgcttata atctggcttc aaaagtggcg aacaatgaaa ttttaaccgc
     1501 ttcttcggga aacttcggtc aggcgctcgc cttagcctgc cagttggtcg gcaaaaaatg
     1561 cacggtcgtg atgccggaaa cttccgcgcg cgtgaaaatc gaagccgtca gaagttacgg
     1621 cgcggcggtc gatttggtcg atacgaataa aatcggcaga aacgaaagag tcgcgcagct
     1681 tgccgccgaa atgccggacg cgtattttgc cagcgcttac gacgatttgt ttgtcatcga
     1741 gggcaattcg acactcggcg acgagctttc aaaaaaagat tttgacgtcg tcgtatcgcc
     1801 cgtcggcggc ggcggtttga tttcgggaat ttccaagggc ttgcgccgca attcgcaccg
     1861 ggcggtttta atcggcgccg aaccgcttct cggaaacgac gctttccgct cgctcaaagc
     1921 gggcgaaatc gtcagaaacg acgtcgagcc gctgtcgatt gccgacggcg cgcgcacgat
     1981 ttcgctcggc aagcacaact gggaaatcat caaaaccgat gtcgccgaaa tcatcgaagt
     2041 ttccgaagaa aaaatcattg aagcggttcg attatatttt tctctggcga atttaaaggt
     2101 cgagccgacc ggcgcgctct cgctcggcgc gattttagaa agcggcgaaa aatttaaaga
     2161 taaaaaagtc tgtctggtcg tcagcggcgg caatgtcgat acggaaatct accggcaaat
     2221 tatcgcctga ttctgaataa gaaggattta gccgcacgga acgccggaaa aaacagggat
     2281 tttaaatgaa aacgcagaga tgaccggata gacgaagata agaaaatgat ttttattatc
     2341 ttcggttcat tcgcattatt actgtcgatt cttttttaaa atctctgttt gcattcggcg
     2401 tttcctgcgg ctgaatctat ctttattttt tctggtaaac gccgctgaaa gtcacgttca
     2461 agccgccgca ttgcagattg tccgtgacaa tcaaatattt gccgagcagg cgcatcgcca
     2521 ctttgcaatc gtattcgccg gtctcgctct cgccgatttt cagctcgttt ccggcaggtt
     2581 ttgattggtc gtcaagctcg cccgtgtgta tgttgtcgcc caagcctttc cagaaagcgc
     2641 ttccggtaat cgtcaataaa cctgccgttt tgcttttgcc gatttcgatt tggcttccat
     2701 tgttgtaatt ccaattgccg agccagtcat ttatcgacgg ctccggattc gtttccttaa
     2761 attcgagttt atcgagcgcc agccagccga ccgtttccca gccttttttc ggctggtacc
     2821 agccgcacgc ccatttgccg aagcggcggg atacgataac ttcatcgcct ggaataacat
     2881 agcttttcgt ttgacagttt ttgtcgttcg ggcaattacg gtcgtcgccg taaaaatgga
     2941 ctttttcgtt tttcgagcct ttgacgctcg ccagtcgata ccgggagctt tcgcgcggga
     3001 aaaacccgtt gcgacacaga ttttcgggat tcccttccgg ctgcgcgaat gtttgaatag
     3061 taaataaaag cagaaccgcg gtcgataaaa taaataattt catagatttt cctttcctca
     3121 cacgctttta ggaacggcta atcgatgttc ttttgcagta tttttcggcg cagataatcg
     3181 agcgccgcct ggctcgaacg ccagcgaatc agctcgcggt cgccgggcag agtcgttttg
     3241 aatgatttcg tttcgctttc gtccgcgtag ccgataaaca
//
LOCUS       DASWNE010000477         1664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_508791, whole genome shotgun sequence.
ACCESSION   DASWNE010000477 DASWNE010000000
VERSION     DASWNE010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508791"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..811
                     /locus_tag="VGB68_09490"
     CDS             65..811
                     /locus_tag="VGB68_09490"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY0049505.1"
                     /translation="MKYIRAIIRLVAFLIATFGSYGLWFVTVYFVPNKLYWRQLTFKS
                     WARLFIKISGMKIEVVGTPPPAPFFLVSNHLSYTDIPLLRAVTGGVFVAKGEIQSWFL
                     GNRIVGDMGNVFVNRRNRRDIPRAGAEVLERLKEGEGVIIFPEGTSSKGEKILKFNSS
                     FLEFAAQTDLPVHHVSITYRTPNNNPPANSICWWAPEDTFIGHLWKLFQIPEFLAIVT
                     FGDEPVRSRNRKELARELREKVNERFVPVL"
     gene            833..1531
                     /locus_tag="VGB68_09495"
     CDS             833..1531
                     /locus_tag="VGB68_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049506.1"
                     /translation="MRLKIIFLILTVLSVSVRLWSAQADNSSKAVLFDKFLKQPYGAT
                     IARIDNFVVHLAYNPNSDGYVVIYPEKNSLKPDFRRGKRLKEIIINRFYQVSGFDEKR
                     FQIVRGEERNELKIELWEVPRGAQKPFSILEILPEKPRDLSKAFIFGSEDIEEAYANF
                     APRRFAKILSENPNLRGHIVIFNKSKKESRLEMQCWLSLFSEDYKLPRSRLKFFFAKN
                     NGRPDAEFWIVPKK"
BASE COUNT          411 a          402 c          399 g          452 t
ORIGIN      
        1 gtaatttata ttcaaccgta agctgacggc tgacggctga ccgctgacgg ctagaaaccg
       61 acttatgaaa tacattcgcg ccataattcg tctggtcgct tttctaatcg ccactttcgg
      121 ctcttacggc ttgtggttcg tcacggttta tttcgttccg aacaagcttt actggcgaca
      181 gttgactttt aaatcgtggg cgcgtttgtt catcaaaatt tcgggaatga aaatcgaagt
      241 cgtcggaacg ccgccgccag cgccgttttt tctcgtctcc aatcacctga gctacaccga
      301 cattccgctg ctgcgcgccg tcaccggcgg agttttcgtc gccaaaggcg aaatccaaag
      361 ctggtttctg ggcaatcgaa tcgtcggcga tatgggaaac gttttcgtca atcgccggaa
      421 ccgacgcgat attccgcgcg ccggcgcaga ggttctggaa agattaaaag aaggcgaagg
      481 cgtgattatt tttccggaag gcaccagctc gaaaggcgaa aaaattctca aattcaattc
      541 ttcttttctg gaattcgccg cgcagaccga tttgcccgtc catcacgttt caatcacata
      601 ccgcacgccg aacaacaatc cgcccgccaa ctccatttgc tggtgggcgc ccgaagacac
      661 gtttatcggg catctctgga aactgttcca aattcccgaa tttctggcga ttgtgacctt
      721 cggcgacgag cccgtccgaa gcaggaaccg caaggagctg gcgcgcgagc tccgggaaaa
      781 agtaaacgag agattcgttc cggttttgta gaaatttccg cgagtctttt atatgcgatt
      841 gaaaataatt tttctcattt taacggtttt atcggtttcc gtccggcttt ggtccgcgca
      901 ggctgacaat tcgtcgaaag ccgttctgtt tgataaattc ctcaagcagc cctacggcgc
      961 aaccatcgcc agaatcgata attttgttgt gcatcttgcg tataatccga attctgacgg
     1021 ttatgtagtc atttatcctg aaaagaactc gctaaagccg gatttcaggc gcgggaaaag
     1081 actcaaagag attatcatta accgtttcta ccaggtttcc ggtttcgatg aaaagcgttt
     1141 tcagattgtt cggggcgaag aaagaaatga gctgaaaata gaactctggg aagttccgcg
     1201 cggcgcgcag aagccttttt ccattctgga aatattgccg gaaaagcctc gagacttatc
     1261 gaaagctttt attttcggtt cggaagacat tgaagaagcg tacgcgaatt tcgcgccgcg
     1321 cagatttgcg aaaattttaa gcgagaatcc gaatttgcgc gggcatatcg tcattttcaa
     1381 caaatccaaa aaagaatcgc gccttgaaat gcagtgttgg ctgagcctgt tttccgaaga
     1441 ttacaaattg ccgcgcagcc ggctgaaatt cttttttgcc aaaaacaacg gcagaccgga
     1501 cgcggaattc tggattgttc cgaaaaagta gatgatttgt ctttcccgtt taccggaagc
     1561 tttaattgga agtttttccg tcttcatact gttccagttt tcgataaagc gtttttctgc
     1621 cgatatttaa taatctcgac gcgcgcgatt tgtcgccgct cgtg
//
LOCUS       DASWNE010000478         2513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_51626, whole genome shotgun sequence.
ACCESSION   DASWNE010000478 DASWNE010000000
VERSION     DASWNE010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51626"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2274)
                     /locus_tag="VGB68_09500"
     CDS             complement(<1..2274)
                     /locus_tag="VGB68_09500"
                     /inference="COORDINATES: protein motif:HMM:NF040218.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEY0049507.1"
                     /translation="MTEKIIAVSRRRKFFITGLLCLLIAGLFAAKQSFISSAQTENEA
                     SPAAVRFSENFDSVTAPQFPPNWTASSTGAGAGFVVTTNTPDTTPNAVYTASPPTTSG
                     TDLTSPSIYVSSVNTILNFRHKFAMENTWDGGVLEISVSGGAFQDIIAAGGTFITGGY
                     NGSLNESSNPLSNRSAWTGATAGGYMTTTIQLPASTFREFVRFKWRFGSNESFGGEGW
                     WIDTITVETISTGANATAITVPGSGIASPYPSEIQISGMTGFITGIIVDLGNFSHTAP
                     DDVDVLLVSPSGRSLVLMSDAGGNNAASGLGITFDDFAPTVLPDNAPLSSDNFKPTNY
                     EPGDTFPAPAPANAPTINSLSGFVGSQPNGTWKLYVVDDTGANAGNLAGGWSITLQSS
                     TAACPFDITSSSQAFPAAGGSAGFQINIPGGCAWTATSDSSFVNVTNAAGEGNGTINF
                     TVAPNTGGGRTASITVSNGLVSRTFLIQQASGCPTSVSSPVLNFPSSQSMAAVFVSAA
                     SNCIWSAVHNANWILIQQKPQPGSGTIIVTATTNPNPTPRTAIITIGVLTITVNQAGT
                     ASAIPARRFDFDGDNKADISVFRQGNWYLQQSSAGFAAVQFGLSTDRIAPADYDGDRK
                     TDIAVFRDGDWYILQSSNNTFRAQHWGAPGSDLPVPADYDGDGRADLAVFRAGAEASS
                     PTYFFILRSSSNAEQTHQFGTTGTDRAFPADYDGDGKADIAVYREAGGIWYILQSSDN
                     NLRGTQFGLGNFQDQPVP"
BASE COUNT          577 a          689 c          719 g          528 t
ORIGIN      
        1 cggtacgggc tggtcttgaa aattgccgag tccgaactgg gtgccgcgca gattattatc
       61 cgagctttgc aggatatacc agatgccgcc cgcttcacga taaacggcga tatcggcttt
      121 gccgtcgccg tcgtaatcgg ctggaaacgc gcggtcggtt ccggtcgttc cgaattggtg
      181 cgtctgctcg gcgttcgacg agctgcgcag gataaagaaa taggtcggcg aggaagcttc
      241 ggctcccgcg cgaaaaacgg ctaaatccgc ccgcccgtcg ccgtcgtaat cagccggaac
      301 gggaaggtcg ctgcccggcg cgccccagtg ctgcgcgcga aaagtattgt tcgagctttg
      361 caggatatac cagtcgccgt cgcgaaaaac ggcgatgtcg gtcttgcggt cgccgtcgta
      421 atcggcgggc gcgatgcggt cggttgacag accgaactga acggcggcaa atcccgcgct
      481 gctttgctga agataccagt tgccctgccg aaagacggaa atgtccgcct tgttatcgcc
      541 gtcaaaatcg aaacgcctcg ccggtatcgc cgatgccgtt ccggcttgat tgaccgttat
      601 ggtcagcacg ccgattgtaa ttatcgccgt gcgcggcgtc ggattcggat tggtggtggc
      661 ggtgacgatt atcgtgccgc tgccgggctg cggcttctgc tgaattaaaa tccagtttgc
      721 gttgtgcacg gcgctccaga tgcaattcga agcggcggag acgaagacgg ctgccatcga
      781 ctgcgaagac gggaaattca gaaccggcga cgaaaccgaa gtcggacagc ccgacgcctg
      841 ctggatgaga aacgtccgcg agaccaagcc gttcgagacg gtgatggaag cggttcgccc
      901 gccgcccgta ttcggcgcga cggtaaaatt aatcgtgccg ttgccttcgc ccgcagcgtt
      961 tgtaacgttg acgaaactcg aatcggaagt cgccgtccag gcgcatccgc ccggaatatt
     1021 tatttgaaaa ccggcgctgc cgcccgccgc cggaaaagcc tgactgcttg acgtaatgtc
     1081 aaacgggcaa gccgccgtcg agctttgcag cgtaatgctc cagccgcccg ccaaattacc
     1141 cgcatttgcg cccgtatcgt ccacgacata aagcttccag gtgccgttcg gctgactgcc
     1201 gacaaagccg ctcaaagagt tgatggtcgg cgcattggcg ggcgcgggag cgggaaacgt
     1261 gtcgcccggc tcgtaattcg tcggtttgaa attatcggaa gaaagcggcg cattgtccgg
     1321 caaaacggtc ggcgcaaaat cgtcgaaagt aattcccaga ccgctcgccg cgttattgcc
     1381 gccggcatcc gacatcagga cgagacttcg accgctcggc gaaaccagca aaacatcgac
     1441 atcatccggc gcagtgtgcg aaaaattgcc gagatcgacg ataatgcccg tgataaatcc
     1501 ggtcattcct gagatttgaa tttcgctcgg atacggcgaa gcgattcctg aaccgggaac
     1561 ggttatcgcc gttgcattgg cgccggtcga aatcgtctct accgtaatcg tatcaatcca
     1621 ccagccttcg ccgccaaaac tttcgtttga gccgaaacgc catttaaacc gcacgaattc
     1681 gcggaaagtc gatgccggaa gctgtatcgt ggtcgtcata taaccgcccg ccgtcgcgcc
     1741 cgtccacgcc gagcgattgg aaagcggatt ggacgattca ttcagagagc cgttgtaacc
     1801 gcccgtgata aatgtgccgc ccgccgcgat tatgtcctga aacgcgccgc cgctgacgct
     1861 gatttccagc acgccgccgt cccaggtgtt ctccatcgcg aatttgtgcc ggaaattcaa
     1921 aatcgtgttg acgctcgaaa catagattga cggcgaggtt aaatcggttc cgctcgtcgt
     1981 cggcgggctg gcggtgtaaa ccgcgttggg cgtcgtgtcg ggcgtattgg tcgtgacgac
     2041 aaagccggcg cccgctccgg tcgaagaagc cgtccagttg ggcggaaact gcggcgcagt
     2101 tacgctgtcg aagttttcgc tgaaccggac ggctgccggg ctcgcttcat tctcggtctg
     2161 cgccgatgaa ataaaagact gtttcgccgc aaaaagaccg gcgattaata aacaaagcaa
     2221 accggtgatg aaaaatttac gacgacgcga aacggcaatg attttttcag tcatggattt
     2281 ttactcaaac tttcagaaag ttatatcggg aaataaaata gagcaatcaa attaaacggg
     2341 cacaccggga aatttgtcaa tgaaaaaagc gcatttttgc cggaaaggag cgccgaatga
     2401 tttttagaaa ttgtgcaaat gcttattttt attcgtttag aggagcgccg gcgctcctct
     2461 aaacgaataa aaataagcat ttgcacaatt tctaaaaatc attcggcgct cct
//
LOCUS       DASWNE010000479         2703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_157367, whole genome shotgun sequence.
ACCESSION   DASWNE010000479 DASWNE010000000
VERSION     DASWNE010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157367"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1395
                     /locus_tag="VGB68_09505"
     CDS             <1..1395
                     /locus_tag="VGB68_09505"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY0049508.1"
                     /translation="SDVITIGGSRLEFERASNDEPTEKLPDAVNYDDQPLGHTQLLIS
                     ASEIIGKRSNLSIETSLVTPEEIKDLRRKAQVLELLYEMSKTLGTVFDLKEIFVKATD
                     LIFRGTPAERVVALIADETVDGKILDYSLFPIAMKARDQAMESLTEKLTISRTITQKV
                     MREKVALLSQDARTDEQFSGSESIVSQGVRSTICAPLITESNVHGVVYADRLDPFAAF
                     SQEDLELISAVAAQTAVTVETVKAHKRLAREEVARANYSRFMPEYVVKQLLENPDSFR
                     LGGVNQTITVLFADIRGFTAISEHENPERIVGLLNRFFSAMSEIIFEHGGTLDKYTGD
                     GLMALFGAPTATPDDAKNALHAAMAMQKRIQTLNEELKNEGFNQIGVGMGLHTGVATV
                     GYIGSDKRSEYTAIGDTVNLASRLESSAQGGQILISEATAKACNEPVSLKAQEPLQVK
                     NRLQPVSLFEVKWS"
     gene            1489..2562
                     /locus_tag="VGB68_09510"
     CDS             1489..2562
                     /locus_tag="VGB68_09510"
                     /inference="COORDINATES: protein motif:HMM:NF010539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY0049509.1"
                     /translation="MKGIASLRNLVTDSMAIDMGTASTIIAVKGRDIVLDEPSLVAVN
                     ELTEEIVAYGQEAYDMQGREGRDVVVIAPLTGGVVADFERTKKMLAYFVRKSKSGGSQ
                     VSTQAVMSMLSEVTHVEQRALMHAAEESHIGKVFMMEEGLAAAFGAGVSPRDKRASAV
                     VDLGASTTNIAIVAKGNVVHARAERIGSNEINLALANHVRRHRGLQIGEETTEKLKTD
                     FASAVLPMDLAKSTIARGRDVQTGSPNAVEITAGEIYPVIEAIIRRVAQIISDTLTEL
                     RPEVAADVFDRGVILTGGGALLDGIDQYLREKVNLPFLISDEPRYATVRGLLQMFDEP
                     NLLEKVTRNEPNLLQNAEVPYEA"
BASE COUNT          681 a          732 c          747 g          543 t
ORIGIN      
        1 agcgacgtca tcactatcgg cggcagccgg ctcgaatttg agcgagcctc gaatgacgag
       61 ccgacggaga aattgcccga cgcggtcaat tacgacgacc agccgctcgg tcacacgcag
      121 cttttaatct cggcaagcga aattattgga aaacgttcaa atttatcaat cgagaccagc
      181 ctcgtcacgc ccgaagaaat caaggattta cgccgtaaag cacaggttct ggaactgctt
      241 tacgagatga gcaaaacgct tggcacggtg tttgacttaa aggaaatctt tgtcaaggcg
      301 acggatttaa tctttcgcgg aacgcccgcc gaaagagtcg tcgcgctgat agccgacgag
      361 accgtggacg gaaaaattct ggattacagc cttttcccga ttgctatgaa agcccgcgac
      421 caggcgatgg aatcgctgac cgaaaaattg acgatttcgc ggacgatcac gcagaaagta
      481 atgcgtgaaa aggtcgcgct gctgtcgcag gacgcgcgca cggacgagca gttttccggc
      541 tctgaaagca tagtttcgca gggcgttcgc tcgacgattt gcgcgcccct gataaccgaa
      601 tcgaatgttc acggcgtcgt ctacgccgac cgcctcgacc cgtttgccgc tttcagccag
      661 gaggatttgg agctgatcag cgcggtcgcg gcgcaaaccg ccgtcacggt cgaaaccgtc
      721 aaagctcata aacgtctggc gcgcgaagaa gtcgcgcggg cgaattacag ccgttttatg
      781 cccgaatacg tcgtcaaaca gcttctggaa aacccggatt cgtttcgcct cggcggcgtc
      841 aaccagacga ttacggtttt gtttgccgat attcgcggct ttacggcgat ttccgaacac
      901 gaaaatccgg aaaggattgt cgggctttta aaccgctttt tttcggcgat gagcgagata
      961 atcttcgaac acggcgggac gctcgacaaa tacacgggcg acggcttgat ggcgcttttc
     1021 ggcgcgccga ccgccacgcc cgacgatgcg aaaaacgcac tgcacgcggc gatggcgatg
     1081 cagaaaagaa ttcagacact gaatgaagag ctgaagaatg aaggctttaa tcaaatcggc
     1141 gtcggcatgg ggcttcacac cggcgtggcg accgtcggtt atatcggctc ggacaagcga
     1201 agcgaataca cggcaatcgg cgatacggtc aatctggcgt cgcggctcga atcaagcgcg
     1261 cagggcggac aaattttaat cagcgaagcg accgcgaaag cctgcaatga gccggtttct
     1321 ctaaaagcgc aggagcctct tcaggtaaaa aatcgcttgc agcccgtatc actatttgaa
     1381 gtaaaatgga gttgaggtag aattagcgaa ccgcaaattt tatgaataat ctctcccaaa
     1441 acaggaaatt tcgattattg cgagtttatt aggaaaagag cagcctctat gaagggaata
     1501 gcatcactca ggaatttggt cacggattcg atggcgattg acatgggcac cgccagcacg
     1561 attatcgccg tgaaagggcg cgatattgtt ctggacgagc cttcgctggt tgccgtcaac
     1621 gagttgaccg aggaaatcgt cgcctacggg caggaagcct acgatatgca gggacgcgaa
     1681 ggtcgcgatg tcgtggttat cgcgcccttg accggcggcg tggtcgccga tttcgaacga
     1741 acgaaaaaaa tgctcgccta ttttgtgcgc aaatcgaaat cgggcggctc gcaggtttcg
     1801 acgcaagccg tgatgagtat gctttcggaa gtgacgcacg tcgagcagcg cgcgctgatg
     1861 cacgcggctg aagaatcgca catcggcaag gtctttatga tggaagaagg cttggcggcg
     1921 gctttcggcg cgggcgtctc tccgcgcgac aaacgcgctt cggcggtcgt cgacctcggc
     1981 gcgagcacga ccaacatcgc catcgtcgcc aaaggaaacg tcgttcacgc gcgcgccgag
     2041 cgcatcggaa gcaacgaaat caatctcgcc ctagcaaatc acgtgcgccg tcatcgcggc
     2101 ttgcaaatcg gcgaggagac gaccgaaaaa ctgaaaaccg atttcgcctc cgccgttctg
     2161 ccaatggatt tagccaaatc gaccatcgcc cgcgggcgcg acgtgcagac cggcagcccg
     2221 aacgccgtcg aaatcaccgc cggcgaaatt tatcccgtta tcgaagccat catccgccgc
     2281 gtcgcccaaa tcatcagcga caccctgacc gaacttcgcc ccgaggtcgc cgccgacgtt
     2341 ttcgaccgcg gcgtgattct aaccggcggc ggcgcgctgc tcgacggcat cgaccaatac
     2401 ctgcgcgaaa aagtaaacct gccttttctc atctccgacg aaccgcgcta cgcgaccgtc
     2461 cgcggacttt tgcagatgtt cgacgagccg aaccttttgg aaaaagtcac gcgcaacgaa
     2521 cccaaccttc tgcaaaacgc cgaagtaccc tatgaagcgt aaaacgtgat tatgcgaaat
     2581 agataatgat aatacgcaaa attgtcgtat tatcaacggt gtcggtgtca ataaccagtt
     2641 agatgcctgt gtttttagct agagcaaaac atgatgagac ttgctgattt gttagagcaa
     2701 ctt
//
LOCUS       DASWNE010000480         4175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_546975, whole genome shotgun sequence.
ACCESSION   DASWNE010000480 DASWNE010000000
VERSION     DASWNE010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546975"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..2332)
                     /locus_tag="VGB68_09515"
     CDS             complement(203..2332)
                     /locus_tag="VGB68_09515"
                     /inference="COORDINATES: protein motif:HMM:NF024972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease family protein"
                     /protein_id="HEY0049510.1"
                     /translation="MFNLNIEPLRQQLFSIVDDRARQQSTVISLPNADGNLEQFEVFE
                     ASNFEPDLQAQFPEIRAFSGRGITDRYATLKLSISPQGIQTMVFRTETKNEFIEPYSK
                     DRTVYAVFKTSREKGDLPWACTTQERDFFSAWKSDIPSAELLTAGSPLSNTGQLKTMR
                     LAQSCNGEYSNFFGATNSSQVGLVLAAFNATMTRANGVYEKDLALHLNLIASTTNVIY
                     YDPATDPYTTLGAWNGQLQTTLTANIGEANYDIGHMFGASGGGGNAGCIGCVCVDGSK
                     GRGITSPADSIPMGDNFDIDYVVHEVGHQLGANHTFSHSLEGSGNNKEVGSGITIMGY
                     AGITSRDVAPHSIDVFHATSIQQIQNNLAPKPCPVTTVMTENHAPVVAPVGNYTIPVT
                     TPFALTGSATDPENDTITYNWEQNDNATTSGANSVASPTKLTGPNWLSFPSTTSPTRT
                     FPRLSTILAGLAVTPPLPGGDAGANIEALSSVSRTLNFRLTVRDNRPYVPASTIGQTQ
                     YTDMTVTVTNTAGPFKVTAQDSFAPNWQGGSQQTITWDVANTNLPPVGTTNVNILLST
                     DGGQTFSTVLASNTPNDGSEQITVPNLPTTTARLKVEAVGNIYFDISNVNFTITAAPV
                     SSVSVSGRVTRANGQAVSNAIVSLVDSGVGGRRVAITNPFGFYRFDNVTTGSAYTIAV
                     ISRLYTFTPQTIQVNNDLTDVNFVAEQ"
     gene            complement(3062..4018)
                     /locus_tag="VGB68_09520"
     CDS             complement(3062..4018)
                     /locus_tag="VGB68_09520"
                     /inference="COORDINATES: protein motif:HMM:NF025224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEY0049511.1"
                     /translation="MRNEVRVRFAVGLIAFCLFAGLGSATVNARQSPNAVESRAGNLK
                     SRAYSAVESGSPEDFENSPCGRFPKAYVFDSFEKAFRSPEKVRCLNPTFEGDDLNMKH
                     LPSRLGALVNLEVLSFGCLEQLEDLPEEIGNLSKLEELIIDNGNGCSMSVSLPNSIGK
                     LERLRVLRLYGAIEPGEQRKIKSLPDSIANLRRLEVLDLGRNGLAAVPPQIAALSNLK
                     TLRLEYNSLSAIPAFIGDFKNLKELSLDANENISDLPASLLKLKGLRISMGNNALKLK
                     AQKSLQSRFPQLVFSFKNEFDDSRANEESPKPRTKSRTRRKR"
BASE COUNT         1022 a         1008 c         1055 g         1090 t
ORIGIN      
        1 tatctattat ctggaacaaa ataaatgccg aaatcagacc cgaaggatta gatactttac
       61 ataaatttgt tctgagcgag acttcacgct gagttcggcg ttataaccaa ttatttattt
      121 catctgaacg gaattttcgg acatcaaaaa aaggcggtct gccggcagac cgcctttaag
      181 tttgttcctg tggattgagg tcttactgct cagccacaaa attcacgtcc gtcaaatcgt
      241 tattgacctg aatcgtctgg ggcgtgaagg tgtagagcct ggaaataacc gcaatcgtgt
      301 aagcgctgcc cgtcgtgacg ttgtcaaagc ggtagaagcc gaacggattg gtaatcgcca
      361 cgcgccgtcc gccgacgccc gaatcgacca gcgatacgat ggcgttgctg accgcctgac
      421 cgttggctct ggtgactcgc ccgctgaccg agacgctcga taccggcgca gcggtaatcg
      481 tgaaattgac attggatata tcaaagtaaa tattaccgac cgcttcgacc ttgagtcttg
      541 ccgtcgtcgt cggcagattc ggaacggtaa tctgctcgct gccgtcattc ggcgtgttgg
      601 acgccaggac ggtcgagaag gtttgtccgc cgtcagttga aagcaggata ttaacattcg
      661 tcgtgccgac cggcggaaga ttcgtattag ctacgtccca ggttatggtt tgctggcttc
      721 cgccctgcca attcggcgca aaagaatcct gagcggtgac cttaaatggt ccggcagtgt
      781 tcgtgaccgt gacggtcata tccgtgtact gggtctgtcc gattgtcgat gccggaacgt
      841 aagggcgatt gtccctgacg gttaacctga agtttaaggt tctggaaaca gagcttaagg
      901 cttcgatatt ggcgcccgcg tcgccgccgg gcagcggagg cgtgacggct aaaccagcca
      961 gaatcgtcga taatctgggg aaagttctgg tcggcgaggt cgtcgaagga aaagataacc
     1021 agttcggacc ggtcaattta gtgggagatg cgacactgtt ggctcccgat gtggtcgcat
     1081 tgtcgttttg ctcccaatta taagtaatcg tatcgttctc cggatcggtc gccgaaccgg
     1141 tcagagcaaa cggagtagtg acaggaatcg tgtaattgcc gaccggcgca acgacgggcg
     1201 cgtggttctc ggtcataacc gtggttaccg ggcagggttt aggcgccaga ttattctgta
     1261 tttgctggat tgaggttgcg tgaaatacat caatcgagtg cggcgctacg tcgcgcgacg
     1321 tgattcccgc ataacccata atggtgattc cggaaccgac ttctttattg ttgccgctgc
     1381 cttcgagtga atgcgaaaaa gtatgattag cgcccagctg gtgtcctact tcgtgaacaa
     1441 cataatcaat gtcgaaatta tcgcccatcg gtattgaatc ggcgggcgag gtaattcctc
     1501 ttccttttga cccgtcgaca caaacacagc cgatacagcc cgcgtttccg ccgccgcccg
     1561 aagcgccgaa catatgaccg atatcataat tcgcctctcc gatattcgcc gtcaaggtcg
     1621 tctgcagctg cccgttccaa gcgccgagcg tcgtataagg gtcggtcgcc ggatcatagt
     1681 aaataacatt ggtcgtgctg gcgataaggt tcagatgcag cgcgaggtct ttttcataaa
     1741 ccccgttcgc ccgcgtcatg gtcgcgttaa aagcggctaa aaccaaacct acctgcgaag
     1801 aattcgtcgc cccgaaaaag ttcgaatatt cgccgttgca ggactgcgcc agccgcattg
     1861 tctttaattg accggtattg cttaaaggcg agcctgcggt aagcagctct gcggaaggaa
     1921 tgtccgactt ccaggcggaa aagaaatctc tctcctgcgt cgtacacgcc cacggtaagt
     1981 cgcctttctc tctggaagtt ttgaacacgg cataaacagt gcggtctttg gaatagggtt
     2041 cgatgaattc gtttttcgtt tccgttctga aaaccatcgt ctgaattccc tgcggagaaa
     2101 tacttaactt taaagtcgcg tatctatccg tgatgcctct tccggaaaag gcgcgaatct
     2161 caggaaactg cgcctgtaaa tcgggttcga aattggacgc ttcgaagacc tcgaactgtt
     2221 cgaggttgcc gtcggcattg ggcagggaaa taaccgtcga ctgctggcgg gctctatcgt
     2281 cgacgataga gaataattgt tgccgcagcg gctcgatatt cagattaaac aatttgaatt
     2341 ctttaggaaa tgattgcctg gcgacagcct tgtcggtagc aatataggat ttctcctgat
     2401 tattctcaga ccagacattt tgctgggcta aaattgcaag attagaaaac gcgataaatg
     2461 tgacaagcaa caaaactagt ttgtatttcc gggcggtata catcgacgat agactcctta
     2521 attaattttt gggattggaa atcgagtttc attctgccca ctcgaatcgg caaaacaagc
     2581 ggcgacagct acttagtaaa acccgaactt tttggaatga atagaatcaa atagagatta
     2641 tatgtcatta taaagaaaaa ttcgggtttt gtccactaat ttcggttccg ttttgggcag
     2701 gacattgtcc ggacacgaaa atccggctgt tagcgtgggc ttttgagtta ataaatattt
     2761 atacgtccat cagatttgag aaggacgatt tgccgctgat gactttatca aaagattgat
     2821 tatcttcgac gcaattaata accgaatggt tgccgcccga taatcggatt ttttcggcgg
     2881 gaagaattaa aaaaaactaa acagcaaagg ttaattccaa tgcagacatt tttcacactt
     2941 cgccctgctg tctgaaaaag tgacgttttt gagccgctgt tttgttatca agcccgatga
     3001 tttagtaccg cgcgccgttt gtcgtaaggc acaaccaaaa cactttcggc tctgacgggt
     3061 tttatcgttt ccgcctggtt cgggattttg tcctgggttt gggcgattct tcattggcac
     3121 ggctgtcgtc aaactcattt ttaaaagaga agacaagttg gggaaaacga ctttgcaggc
     3181 ttttctgagc tttcagctta agcgcgttgt ttcccatcga gattcttaag cctttaagtt
     3241 ttaagagcga cgcaggcaga tcggatatgt tctcgttggc gtccagagaa agttctttca
     3301 ggtttttaaa atcccctata aaggctggga ttgcgctcag gctgttatat tcgagccgca
     3361 atgttttcag gtttgacaaa gcggcgattt gcggcggaac ggctgcaaga ccatttctgc
     3421 ccagatccaa aacttccagt cggcgaagat tggcgatact gtccggcaag cttttgattt
     3481 tacgctgctc gccgggctca atcgcgccgt acaatcgcaa tactctcaag cgttccaatt
     3541 tgccgatcga gtttggcagc gaaacgctca tcgagcatcc gttgccgtta tcaatgatta
     3601 attcttccag cttcgagagg tttccgattt cctccggcag gtcttcaagc tgttccagac
     3661 agccgaaact taacacttcc agattaacca aagcgccgag gcgcgacggc agatgtttca
     3721 tatttaagtc atcgccttca aaggtcggat taagacacct gactttttcg ggcgaccgaa
     3781 aagccttttc aaaactgtcg aaaacatacg ctttcggaaa acgcccgcac ggagaatttt
     3841 caaaatcctc cggactgcct gattcgaccg ccgaataggc gcggctcttt aagttgccgg
     3901 cacgcgattc gacggcgttc ggtgattgcc gcgcgtttac cgtcgccgag ccgagtccgg
     3961 cgaaaagaca gaaggcaatc aatccgactg caaaacgaac ccgtacttca tttctcataa
     4021 ttttttctca tactccgcgt cctgttctgg ttttccggat aatcggcatc tgccggcagc
     4081 tttgcgaaag agggaaataa cgcgttcgcg ccggcataga gtgataaata ccatccgctt
     4141 tcgtgaacgg cggaaaaatc cgtatgttca tcatc
//
LOCUS       DASWNE010000481         1746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_581400, whole genome shotgun sequence.
ACCESSION   DASWNE010000481 DASWNE010000000
VERSION     DASWNE010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581400"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..900)
                     /locus_tag="VGB68_09525"
     CDS             complement(34..900)
                     /locus_tag="VGB68_09525"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY0049512.1"
                     /translation="MEIAEENKSVHRGLKKGLYLIPSAFTAANIGMGFLAVLSSVRGF
                     QMVVQSPEQAAVYFDYAAKAIGFAILFDTLDGRVARMTKTTTEIGVQLDSLADVLTFG
                     IAPIVLAYSWAIGAAFHEESGIHGLGMFVLFMYLMCGTFRLARFNLQATRPRVLIEGT
                     TKIDKKNFVGLPIPPAAGLLAGLVHFAPLPLNAYGENLSALYGSLILVLLAVLGLLMV
                     STLRYTSFKTTAAGRRSLYLILLIAATGMLVWLYSRYVLLALALAYVSHGIVWYMLNL
                     FRRPKQVEEVQT"
     gene            complement(1051..1716)
                     /locus_tag="VGB68_09530"
     CDS             complement(1051..1716)
                     /locus_tag="VGB68_09530"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HEY0049513.1"
                     /translation="MFQSDSRLPTPDRRTFPMVKEGFPFVIVPLLIALIFAFFYLWVG
                     VVAFVLLAAFMAFFFRDPRRTIPTDENIIVSAADGRVTRVEETDEGKLVSVFLSPLNV
                     HINRSPISGKITGALYTQGKKIPATRNEASFVNERNSLVIEGEKMTVTCTQIAGIVAR
                     RIVCWKRAGDSLEIGEKFGLIKFSSRTDLLMPKNVEILVKIGDKVVGGETIIARIANK
                     TTG"
BASE COUNT          456 a          472 c          431 g          387 t
ORIGIN      
        1 tcccgcattt tccgttttta cgtttttgat tgattacgtt tgaacttctt cgacctgttt
       61 cgggcggcga aacaaattca gcatatacca gacgatgccg tgcgagacgt aagccaaagc
      121 caatgccagc aagacgtagc gcgaatagag ccagacgagc attccggtcg cggcgattaa
      181 caaaatcaga tacaggctgc ggcgaccggc ggcagtggtt ttgaagctcg tgtagcgcag
      241 cgtgctgacc atcagaagtc cgagaacggc gagcaaaacc agtattaacg agccgtaaag
      301 cgcgctcaga ttctcgccgt aagcgttcag cggcagcggc gcgaaatgga cgagtccagc
      361 cagcagaccg gcggcgggcg gaatcggcaa accgacaaag ttctttttat caatcttggt
      421 cgtgccttca atcaaaacgc gcggtctagt cgcctgcaaa ttaaatcgcg ccagtcgaaa
      481 cgtgccgcac atcagataca taaacagcac gaacattccc agcccgtgaa ttccgctttc
      541 ctcgtgaaac gccgcgccga ttgcccagct gtaagccaga actatcggcg cgatgccgaa
      601 agtcagaacg tcggctaacg agtcgagctg gacgccgatt tccgtagtcg ttttcgtcat
      661 ccgcgcaact cgaccgtcga gcgtgtcgaa caaaatggcg aacccgatgg ctttcgccgc
      721 gtaatcgaaa taaacggcgg cttgttccgg actttgcacg accatctgaa aaccgcgcac
      781 cgaggacaaa actgccagaa aacccatccc gatatttgcc gccgtaaacg ccgacggtat
      841 caggtaaaga ccttttttca agcccctgtg aacggattta ttttcttcgg caatttccat
      901 aactagagat aatttaccac agcgccgccg ctgacgcgga aaaaattaaa acgggacgga
      961 caggatttac aggataaatt caccttaatt aaagttccgg gttattccgt ccatcctgtt
     1021 catcctgttt taaatccgcg tttatctgtg ttaacccgtt gttttattcg caatccgcgc
     1081 gataatcgtt tcgccgccga cgaccttgtc gccgattttt accagaatct cgacgttttt
     1141 cggcattagt aaatcggtgc gcgagctgaa tttgattaag ccgaattttt cgccgatttc
     1201 caaagaatcg ccagcccgtt tccagcagac aatgcggcgc gccacaattc ccgcaatctg
     1261 cgtgcaggtg acggtcattt tttcgccttc gataaccagc gagtttcttt cgttgacgaa
     1321 gctcgcctcg ttgcgcgtcg ccggaatttt tttgccctgc gtgtaaagcg ctccggtaat
     1381 ttttccgcta atcggcgagc ggttgatatg aacgttcaga ggcgacagaa aaacgctgac
     1441 cagttttcct tcatccgttt cctcgacccg cgtcacgcga ccgtcggcgg ctgagacgat
     1501 aatgttttca tccgtcggaa tcgtccgccg cgggtcgcga aaaaagaacg ccataaaagc
     1561 ggcgagcaaa acaaacgcga cgacgccgac ccaaagataa aaaaaagcga aaatcagcgc
     1621 gataagaagc ggaacgatga caaatggaaa tccttcttta accatcggaa aagtccgtcg
     1681 gtccggagtc gggagtcgag agtcagattg aaaaatctaa gaccgaagac cgaagaccaa
     1741 agaccg
//
LOCUS       DASWNE010000482         9154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_264095, whole genome shotgun sequence.
ACCESSION   DASWNE010000482 DASWNE010000000
VERSION     DASWNE010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9154
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264095"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VGB68_09535"
     CDS             complement(<1..182)
                     /locus_tag="VGB68_09535"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEY0049514.1"
                     /translation="MKTKQKQNKPTAGELEILDVIWERGEATVREVYEALNIRKPGAY
                     TTVLKLMQIMHEKGLV"
     gene            complement(296..1702)
                     /locus_tag="VGB68_09540"
     CDS             complement(296..1702)
                     /locus_tag="VGB68_09540"
                     /inference="COORDINATES: protein motif:HMM:NF025135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4166 domain-containing protein"
                     /protein_id="HEY0049515.1"
                     /translation="MINPTDKNQIEAIRYLPQGVEAERRAFTARQRRARLRLARLKAF
                     YEENYCRLTRLHLLAACAIALGRFCGISAAFDEIGESLMPVDAENAPLTEDWENYVGA
                     CRAKMPLEVREELWLTAHFKDIENVRRKNLEAKFYEEAENYKNEPIWQFLRQHLEVTL
                     GVRLMDKSALAGLIPNETAIAGSLSLTVKKLSTVASKLVFRYAKESLRAVFDFSGETE
                     KDVLSYRQTAFAMTRYFFLSFGVSGIFRQGIRNRERGRIAEIENWALLDAILGDRIGA
                     LNPLIREFYANPSRFDATATLKLETLPLRFWSRSLTLLLGQGLYEEDLNEIPARFRIF
                     ERKDGSMHFVRELFCDGKYRVFDADFVVRDGKLYEVFTDLKVAIEMSVEPFGGDGLSI
                     KSRRVLFHGLSMPTAGIKVDFQSRVEKDENGNELLKVEGLLFMRPETRLGAFFAHKIL
                     RRPKNLGSISYKAWRKAL"
     gene            1796..3373
                     /gene="nadB"
                     /locus_tag="VGB68_09545"
     CDS             1796..3373
                     /gene="nadB"
                     /locus_tag="VGB68_09545"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF005701.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEY0049516.1"
                     /translation="MALETDFIVIGSGVAGLRASVEIARTGASVTVLTKDKASESNTE
                     YAQGGVAVVLSDDDNAELHEEDTLAAGAGLCDEKAVETLVNEGAKYIRQLIEWGTEFD
                     REGGKLIFTREAAHSRRRILHAHGDSTGKEIVRALIARARTEKTIVLMPFANTESLIV
                     SENRCVGVRFLDPILRAPREIFAKAVILCTGGAGQLYMHTTNPTVATGDGMAMAYFAG
                     AEMADMEFVQFHPTALNLENAPRFLLSEAMRGEGGILRNKYGERFMSRYDERLELAPR
                     DIVSRSIVAEMRRTGTRTVFLDMTALDEDFLKNRFPKIYETCEFYGLNIAKDLLPVSP
                     ASHYCMGGVRTDLQGRTSVAGLYAAGEVSCTGVHGANRLASNSLLEGLVFGARSGEAA
                     IEDNAKRKIQNAQSEPQNSALGARHSLEISTAVKKRVKRVMWERVGILRDRDSLKRAL
                     AEFEQISEANLSVSSRNFVTLAMLVTRAALWREESRGGHFRTDFPERSEKWRVHSIQK
                     TEAEISASEKINFAATV"
     gene            3438..3893
                     /locus_tag="VGB68_09550"
     CDS             3438..3893
                     /locus_tag="VGB68_09550"
                     /inference="COORDINATES: protein motif:HMM:NF023520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3574 domain-containing protein"
                     /protein_id="HEY0049517.1"
                     /translation="MNKFPIKQILAAGLFLAFLSAATYAEPPFFARSGQSGKLFVFDS
                     YLRTELFFGTAKADGSAVSEAEWQKFLADEVTPRFPAGFTVLAGDGQFSDEGKIIREK
                     SYVLIVLYPLKTRRGSSRKIERIRSAYIKAFQQKSVMRVDFPQIMQVSF"
     gene            complement(4036..5718)
                     /locus_tag="VGB68_09555"
     CDS             complement(4036..5718)
                     /locus_tag="VGB68_09555"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEY0049518.1"
                     /translation="MSANVGTLDNVAAAGDDVRTHPRGLYVLFGTEMWERFSFYSMLA
                     LFTLYLRDPTEGFGWTATQATSLYANYLMFVYASPLIGGLIADKITGYRKAVMIGGFF
                     FMAGHGLLSIPAIWAVYAALTCLVIGNGFFKPNVSTMVGNLYPEGSHLKDRAYNIFYM
                     GINVGAMLAPIVMEIVKAYFGFHAAFAVAAFGMVISVAILWFFKKEVENTDKRNPLAS
                     PNQAADTAATATDAPPHGISDQDEGGHRAANAARQDRMNAVPDWKRVMALIVIFIIVI
                     VFWMVFHQNGSTLTYWADDNTAWNVSGTISNSINPFWIIVLTFPLVAFWGWLDKRGME
                     PATPTKMAIGMLLTGLSFFVLYGAAKIGENQPVTPEQYASGSFRINERVAGNLKADGV
                     PQDVLDKMLAAKDADGKNVINDVKFSAKEDETTKVKITGEQSYIDTINKVVPGAGTQY
                     RDKFLQRSYLFQVSPFWLLLAYAIISLGELMLSPMGLSLVSKVAPIRMRGLMMGGWFV
                     ATAIGNKLTMIGIYWSIWYQSSFFIVLGAMAVVMSIVLFFLLKPLKKAMPGV"
     gene            complement(5862..6317)
                     /locus_tag="VGB68_09560"
     CDS             complement(5862..6317)
                     /locus_tag="VGB68_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049519.1"
                     /translation="MADKTTKMRNANFIKKTNGKFTAGHNTPNAVDIEYDGLDEEIKS
                     IFDNLRLVGSPQEYSLDEVLVKAIGTEKKSLENNVKISDGKPSSGIFGKDGKSGRKQI
                     FKIWDVELQFQVATMLILTLGSIYLVFILPLKGQEVTKHFREIQVQVIK"
     gene            complement(6307..6909)
                     /locus_tag="VGB68_09565"
     CDS             complement(6307..6909)
                     /locus_tag="VGB68_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049520.1"
                     /translation="MLFDDGISGREKSAEDESQRKLEIVIKEISEKSFSKYSLLEDRV
                     ISRGKSIYDVIQDKLFEFVKRSRKPGANPQDDSKWIDRNIIQGKRKLYKYSDDIASIN
                     DDDFNSRHFAQPSKDQSKLEKYEIIRKVLSNYNARNKLIVDLSFYVGLENREIAKKAN
                     TTEGNVRKVIHDFKKDCRKFGIESSEGRPGENIGDKNNGR"
     gene            7110..7592
                     /locus_tag="VGB68_09570"
     CDS             7110..7592
                     /locus_tag="VGB68_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049521.1"
                     /translation="MMKTAPALLSIALFLFVFGLPTENRAQTRKAVGAAEASGTFRSH
                     FKGRFKDFYNEIKILTLGKGKLQVAFELMYPHLGGGEVTANTGTAEGIAEIKGDTAVF
                     TNKEFGACRIIIKFVKPGVIKVTQSISAGECGFGFNVRADGTYKKTSRAKPKFGRQQP
                     "
     gene            7708..8451
                     /locus_tag="VGB68_09575"
     CDS             7708..8451
                     /locus_tag="VGB68_09575"
                     /inference="COORDINATES: protein motif:HMM:NF025580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4328 domain-containing protein"
                     /protein_id="HEY0049522.1"
                     /translation="MQSENAVQYRSPRVLAYLAMFFLGSLFITNLLSMFFGFGEIFFS
                     RAIEIESGEYMSVWLIFIGLISILELFLYVAAVVFFLVWLFRVYKNLTPLRAPNPEFT
                     PGWAVGWWFIPFANLVKPFQVVREAWQQSDPDFDPSLNFLSGGVGSTPAFLGFWWAFW
                     ILSNIANNIASRLMDSNNSTASDGFAAAWIIASVLSMIAAILAVLVVKSITARQDERY
                     QNLFKMQVYSPPAPPNFNQPNFNQPNQTF"
     gene            8454..>9154
                     /locus_tag="VGB68_09580"
     CDS             8454..>9154
                     /locus_tag="VGB68_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049523.1"
                     /translation="MELFQKLGAQIELLWREKNYDEELFPEIAARALEEADLPQKVSA
                     WDVIDWTLRQTVLPEQRDLSARFADPPITLYNSPRFHVDVYFWLEGTTSIHQHGFCGA
                     FQVLLGSSLHSWYDFECRAKVNIFTEIGDMKLKACRLLGVGDVQEIRAGRKYIHGLFH
                     LDQPSATIVVRTHRSPLAMPQYNYEKPHLAIDPFFEEPATIKKLQALTALIRSNHPDT
                     DARIVEWLETADFHTS"
BASE COUNT         2216 a         2309 c         2304 g         2325 t
ORIGIN      
        1 tcgacaagac ctttttcgtg cataatctgc atcaatttca aaacggtcgt ataagcgccg
       61 ggcttgcgga tgtttaaagc ttcgtaaact tcgcgcactg tcgcctcgcc gcgttcccaa
      121 atcacgtcga gaatttccag ctcgcccgcc gtcggtttgt tttgcttttg tttcgttttc
      181 atatcagcgc ctacgaagaa gttactacga agtttttcgt atgtcaatgc gattgtcgaa
      241 aaaaatttgc cggagatgtt ttcgagcgag cggaaagatt gtctaaaacc gaattttaaa
      301 gcgcttttcg ccacgcttta taactaatgc ttcccagatt tttcggtcgg cgcaggattt
      361 tatgcgcgaa aaacgcgccg agtctggttt cgggtcgcat aaataaaagc ccttcgactt
      421 ttaacagctc gtttccgttt tcgtcttttt cgacgcggct ttgaaaatcg actttaatgc
      481 cggcggtcgg catcgaaagt ccgtgaaaca aaacgcgacg gcttttgatt gataaaccgt
      541 cgccgccgaa aggctcgacg ctcatttcaa tcgccacttt caaatcggta aaaacttcgt
      601 agagctttcc gtcgcggacg acgaaatcgg cgtcgaaaac gcgatacttg ccgtcgcaaa
      661 aaagctcgcg gacaaaatgc atcgagccgt ctttgcgctc gaaaatgcga aagcgcgccg
      721 gaatttcatt caaatcttct tcgtacaaac cctgcccgag aagcagggtc aaagaacgcg
      781 accagaaacg caggggcaag gtttcgagct tcaaagtcgc cgtcgcgtcg aaccgcgatg
      841 gattggcata aaactcccgg attaaaggat ttaacgcgcc gattcggtcg cccaaaatcg
      901 cgtcgagcag agcccagttt tcaatctcgg cgattctgcc gcgttcacga ttgcgaattc
      961 cctgccggaa aattccggaa acgccgaacg acaggaagaa atatcgcgtc atagcaaaag
     1021 cggtttgccg gtagctcagg acatcttttt ccgtttcgcc ggaaaaatcg aaaacggcgc
     1081 ggagactttc cttcgcatag cggaaaacga gctttgaagc gaccgtcgag agttttttga
     1141 cggttaagga gaggcttccg gcaatggcgg tttcattcgg aatcaaaccg gcgagcgcgg
     1201 atttatccat cagccgaacg cccagagtaa cttccagatg ctgccgcaga aactgccaaa
     1261 tcggctcgtt tttataattt tccgcttcct cgtaaaattt ggcttccaga ttttttcggc
     1321 gcacgttttc gatgtctttg aaatgcgcgg tcagccagag ctcctcgcgg acttccaaag
     1381 gcattttagc gcggcaagcc ccgacgtaat tttcccagtc ttcggtcagc ggcgcgtttt
     1441 cggcatcgac cggcatcagc gattcgccga tttcgtcgaa agcggcggag attccgcaaa
     1501 atcttccaag cgcgatggcg cacgccgcca gcaggtgaag ccgcgtcagc cgacaataat
     1561 tttcctcgta aaacgctttg agccgcgcca gccgcagacg cgcgcgcctc tgccttgcgg
     1621 taaacgcccg gcgctccgct tcgacgccct gcggcaggta gcgtatcgct tcgatttgat
     1681 ttttgtcggt cggatttatc acctggcaga gaaattgtat ccgcaaccgt aatttaagca
     1741 aatgcggggg cgttgaattt gaaattttcg aggcgagcat ccacaatttc aaaatatggc
     1801 actggaaacc gattttatcg tcatcggaag cggcgtcgcc ggactgcgcg cttcggtcga
     1861 aatcgcgcgg acgggcgcga gcgtgaccgt tttgaccaaa gacaaagcga gcgagtcaaa
     1921 caccgaatac gcgcagggcg gcgtcgcggt cgtcctgtcg gacgatgata acgccgagct
     1981 tcacgaggaa gacacgctgg cggcgggcgc gggattgtgc gatgaaaaag cggtcgagac
     2041 gctcgtcaac gaaggcgcga aatatatcag acagttaatc gaatggggaa cggaattcga
     2101 ccgcgaaggc ggaaaactga ttttcacgcg ggaagcggct cattcgcggc ggcgaatcct
     2161 gcacgcgcac ggcgattcga ccggaaagga aatcgtgcgc gccctgattg cgcgcgcgcg
     2221 gacggaaaaa acgattgttc tgatgccgtt tgccaatacg gaaagtttga ttgtttccga
     2281 aaacaggtgc gtcggcgttc gctttctcga cccgattttg cgcgccccgc gcgagatttt
     2341 cgctaaagcg gtcattctct gcacgggcgg cgcgggtcag ctctatatgc acacgacgaa
     2401 tccgacggtg gcgaccggcg acgggatggc gatggcttat tttgcgggcg cggagatggc
     2461 tgatatggaa tttgtgcagt ttcatccgac agctttgaat ttggaaaacg cgccgcgttt
     2521 tttattgagc gaggcgatgc gcggcgaagg cggaattttg cgcaacaaat acggcgagcg
     2581 ttttatgtcg cgctacgacg agcggctgga attagctccg cgcgatatcg tttcgcgctc
     2641 gattgtcgcg gaaatgcggc gaaccggaac gcggacggtt tttctggata tgacggcgct
     2701 cgatgaagat tttctcaaaa atcgctttcc gaaaatttat gaaacgtgcg aattttacgg
     2761 cttgaatatt gcgaaagatt tactgcccgt ctcgcccgct tcgcattatt gtatgggcgg
     2821 cgtgcgcacg gatttgcagg gcagaacgtc ggtcgccggg ctttacgcgg cgggcgaggt
     2881 ttcgtgtacg ggcgttcacg gcgccaaccg gctcgcttcg aattcgctgc tcgaaggctt
     2941 ggttttcggc gcgcgctccg gcgaagcggc gattgaagac aatgcaaaac gcaaaataca
     3001 aaatgcacaa tcggagcctc aaaactcggc gctcggcgct cggcattcgt tggaaatttc
     3061 gacggcggta aaaaaacgcg tcaagcgcgt gatgtgggaa cgggtcggaa ttttgcgcga
     3121 cagggattct ctgaaaagag cattggctga attcgagcag atttcggaag cgaatttaag
     3181 cgtttcctcg cgcaactttg tcacgctggc gatgctggtc acgagagccg cgctgtggcg
     3241 cgaagaatcg cgcggcgggc attttcgcac ggactttccc gagcgcagcg aaaaatggcg
     3301 cgttcattcg attcaaaaaa ccgaagcgga aatttcggcg agcgaaaaaa taaactttgc
     3361 ggcgaccgtt tgaacgcgcc gcaaaatttc cctttaaatt taataaattc tcctttaaat
     3421 ttaataaggt gtagaaagtg aataaattcc cgataaaaca aattttagca gccggtttat
     3481 tcctggcatt tttgtcggcg gcgacttatg ccgaaccgcc gtttttcgct cgttcaggtc
     3541 aaagcggaaa gctttttgtc tttgactctt acctgcgcac cgaattgttt ttcggaacgg
     3601 ctaaagcgga cggctctgcc gtttccgaag cggaatggca aaagtttctg gctgatgaag
     3661 tgacgccgcg ttttccggcg ggatttaccg ttctggcggg cgacgggcaa tttagcgacg
     3721 aggggaaaat catccgcgaa aagagctatg ttttaatcgt gctctacccg ctgaaaacgc
     3781 ggcgcggcag cagccggaaa atcgaacgga ttcgcagcgc ttatattaaa gcctttcaac
     3841 aaaaatcggt gatgcgcgtg gactttccgc aaattatgca ggtttcgttt taagaaacgg
     3901 cgaatttcat tttaagaaat tctcgaccgc cgccgatacg aagaaagcag attcaaatgc
     3961 gcttcgggtt aaataaaaaa actgcgcttc ccgaatgaga aacgcagttt tcgtttaatc
     4021 agccggtgcc gaacgctaga cgccgggcat cgcttttttc aaaggcttga gcaggaaaaa
     4081 cagcactatc gacatcacca ccgccatcgc gccgagcacg atgaagaacg acgattgata
     4141 ccagatgctc cagtaaattc caatcatcgt cagcttattg ccgatagccg tcgccacaaa
     4201 ccagccgccc atcatcagcc cgcgcattct gataggcgcg acttttgaga ccagcgacaa
     4261 acccatcggc gacaacatca attcgccgag cgagataatc gcgtaagcca gcagcagcca
     4321 gaacggcgat acctgaaaca ggtaactgcg ctgcaaaaac ttatcgcgat actgcgttcc
     4381 cgcgcccggc acgactttgt ttatcgtatc tatataactc tgctcgcccg tgattttcac
     4441 tttcgtcgtt tcgtcttctt tggcggaaaa cttcacgtcg ttgatgacgt tttttccgtc
     4501 cgcgtccttg gcggcgagca ttttatccag aacatcctgc ggaacgccgt cggctttcag
     4561 gttgccggcg acgcgctcgt tgatgcggaa tgagcccgac gcgtattgtt cgggcgtgac
     4621 cggctggttt tcgccgattt tcgccgcgcc gtaaagcacg aaaaacgaca atccggtgag
     4681 cagcattcca atcgccattt tcgtcggcgt ggcaggctcc attccgcgtt tatcgagcca
     4741 accccagaag gcgacgagcg gaaatgtcag aacgataatc caaaacggat taatcgagtt
     4801 cgagattgtt ccggaaacgt tccaagccgt gttgtcgtcc gcccagtaag ttaaggtcga
     4861 gccgttttga tggaagacca tccagaagac gatgacgatg ataaaaatca caatcagcgc
     4921 catcacgcgc ttccagtccg gcacggcgtt catccggtcc tggcgcgcgg cattggcggc
     4981 tctatgcccg ccttcatcct ggtcgctgat gccgtgcggc ggagcgtccg tcgccgtggc
     5041 ggcggtgtct gccgcttgat taggcgacgc gagcggattt cttttatccg tgttttcgac
     5101 ttcttttttg aaaaaccaga gaatcgccac cgagatgacc attccgaaag cggcaacggc
     5161 aaacgcggcg tggaaaccga aatacgcttt gacgatttcc ataacaatcg gcgccagcat
     5221 agcgccgaca ttgatgccca tataaaaaat gttataggcg cggtctttca aatggctgcc
     5281 ttccgggtaa aggttgccga ccatggtcga gacgttcggc ttgaaaaatc cgttaccgat
     5341 aaccagacag gtcagcgcgg cgtaaaccgc ccagattgcc ggaatcgaaa gcagcccgtg
     5401 tccagccata aagaaaaaac cgccaatcat cacggctttg cgatagccgg tgattttatc
     5461 ggcaatcaag ccgccgatga gcgggctggc ataaacaaac attagataat ttgcgtaaag
     5521 cgaggttgcc tgcgtcgccg tccagccgaa gccttccgtc gggtcgcgca aataaagcgt
     5581 aaacaatgcg agcatcgaat aaaaactgaa acgctcccac atctccgtcc cgaaaagcac
     5641 gtaaagtcct ctcgggtgcg tccgcacgtc gtcacccgcc gcggcgacgt tatctaaagt
     5701 tccaacattt gcactcatta atttttcccc tcggtttatt taaattttaa aaagaattgg
     5761 gcaaaatata gcagataaac ctcttaaaac aaagaataat ttacagcaat cattctccgg
     5821 cgattaaaat aacgccgccc gtttcgctta tttcgggcga gtcattttat gacttgaact
     5881 tgaatttccc ggaaatgttt cgttacttcc tgacctttta acggtaaaat aaatacaagg
     5941 taaatacttc ccaaggtcaa aatcagcatt gtcgccacct gaaattgaag ctctacatcc
     6001 caaattttaa atatttgctt tctgccggat ttcccgtcct ttccgaatat gccggaagat
     6061 ggttttccgt cagaaatttt tacattattt tccaacgatt tcttctccgt gccgatagct
     6121 ttaacaagca cttcatcgag ggaatactcc tgcggacttc caactaatct caggttgtcg
     6181 aatatgcttt taatctcttc atccaagccg tcatattcga tatccacggc gtttggagta
     6241 ttgtgcccgg cagtaaattt accatttgtt ttcttgatga aattcgcatt tctcattttt
     6301 gttgttttat ctgccattgt ttttatctcc aatattttcg cctggtctgc cttcggaaga
     6361 ctcgatgcca aatttacgac agtctttttt aaaatcatga attacctttc tgacatttcc
     6421 ttccgttgta ttagcttttt ttgcaatttc cctgttttcg agcccgacat aaaagctgag
     6481 gtcaacaatt agtttgtttc tggcattata attacttaag actttccgga taatttcata
     6541 tttttccagc ttgctctggt ctttagacgg ttgagcaaaa tgcctggaat tgaaatcgtc
     6601 gtcgtttatc gaggcaatat catccgagta tttataaagc tttctttttc cttgaatgat
     6661 gtttctgtca atccatttcg agtcgtcctg agggttggct ccgggctttc ggcttctttt
     6721 cacaaactcg aaaagcttat cctgaatgac atcataaata gatttgcctc tcgatattac
     6781 ccggtcttcc agaagggagt attttgagaa actcttttct gaaatttcct tgatgactat
     6841 ttccaatttt ctttgacttt catcttcggc agacttttct ctgccggaga ttccatcgtc
     6901 aaataacata aaacacccac tttatttccg actcataaca ttcaccattc ttttatgaaa
     6961 aaatctgctt taatatacta aaggaaataa aaatcagttt tgttacgaat ttgttctaaa
     7021 gatgatgaaa aaaattcggg aagccgtctg aaacaatttg ttttcaggac aattttttcg
     7081 tacccaatca aaaggaaaac attgaaaata tgatgaaaac agctccggca ttgttgtcta
     7141 tcgccctatt tttattcgtt ttcggcttgc cgaccgaaaa ccgcgcgcaa acccggaaag
     7201 ccgtcggcgc ggcggaagcg agcggcactt ttcgctcgca tttcaaaggc agattcaaag
     7261 acttctacaa cgaaatcaaa attttgacgc tcggcaaggg caaattgcaa gtcgctttcg
     7321 agctaatgta tccgcatctc ggcggcggcg aggttacggc gaatacgggc acggcggaag
     7381 gcatcgccga aataaagggc gacacggcgg tttttacgaa caaagagttc ggcgcgtgcc
     7441 ggattatcat caaattcgtc aaaccgggcg taatcaaggt gacgcaatca atcagcgcgg
     7501 gcgagtgcgg cttcggattt aacgtcagag ccgacggcac gtataaaaaa acgagtcggg
     7561 caaaaccgaa attcggtcgg caacagccgt aatttcagac ggcggacggt tgcgaaaacg
     7621 caaactttct gcgagtattt tatcgaaacc gacacgaatc gaatcataat ttttaactaa
     7681 acctttcctt ttcaattcag gaggcagatg caatcggaaa acgctgtaca atatcgctcg
     7741 ccgcgagttt tagcatatct cgcgatgttt tttctcggca gcttgtttat aaccaatctg
     7801 ctcagtatgt ttttcggctt cggcgagatt tttttctccc gcgctatcga gatcgagagc
     7861 ggcgaatata tgtccgtctg gctcattttt atcggcttga tcagcattct ggagcttttt
     7921 ctgtacgtcg cggcggtcgt gttttttttg gtctggcttt tccgcgttta taaaaatctg
     7981 acgccgctgc gcgcgccgaa tccggaattt acgccgggct gggcggtcgg ctggtggttt
     8041 atcccttttg ccaatctggt caagcctttt caggtcgtgc gcgaggcgtg gcagcagagc
     8101 gacccggatt tcgacccgag cctgaatttt ctttccggcg gcgtcggctc gacgccggca
     8161 tttctgggtt tctggtgggc gttctggata ctttccaaca tcgcgaacaa tatcgcctcg
     8221 cggctgatgg attcgaataa cagcacggcg tcggacggtt ttgcggcggc gtggattatc
     8281 gccagcgttt tatctatgat tgcggcgatt ctggctgttc tggtcgtcaa gagcatcacc
     8341 gcgcggcagg acgagcgtta tcaaaacctt tttaaaatgc aggtttattc gccgccggcg
     8401 ccgccgaatt ttaatcagcc gaattttaat cagccgaatc aaacttttta gagatggaat
     8461 tatttcaaaa gctcggcgct caaatcgagc tgctctggcg cgaaaaaaat tacgacgagg
     8521 agctttttcc ggagattgcg gcacgggcgc tcgaagaagc cgatttgccg cagaaggttt
     8581 cggcgtggga tgttatcgac tggactctgc gccaaaccgt tctgcccgaa cagcgcgatt
     8641 tgtccgcgag gtttgccgac ccgccgataa cgctctacaa cagcccgcgt tttcacgtcg
     8701 acgtttattt ctggctcgaa ggcacgacct cgattcacca gcacggtttt tgcggggcgt
     8761 ttcaggttct gctcggttcg agcctgcata gctggtatga tttcgagtgc cgcgcgaaag
     8821 tcaatatttt cacggaaatc ggcgatatga aattaaaagc gtgtcgtctg ctcggcgtcg
     8881 gcgacgtgca ggaaattcgc gccggcagaa aatatattca cgggcttttt catcttgacc
     8941 agccgtcggc gacgattgtc gtccgcacgc atcgcagccc tttggcgatg ccgcagtata
     9001 attacgaaaa accgcacctg gcgattgacc cgttttttga agaaccggcg acgattaaaa
     9061 aactgcaggc gctgacggcg ctgattcgct cgaatcatcc cgacacggac gcgcgaatcg
     9121 tcgaatggct cgaaacggca gattttcaca cgtc
//
LOCUS       DASWNE010000483         2630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_157525, whole genome shotgun sequence.
ACCESSION   DASWNE010000483 DASWNE010000000
VERSION     DASWNE010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157525"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2134)
                     /locus_tag="VGB68_09585"
     CDS             complement(<1..2134)
                     /locus_tag="VGB68_09585"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY0049524.1"
                     /translation="MIHKVFQLNSHSFFSFILVSLVLHVVSFNVGCQPMVSRMTEEQA
                     LQAIRQLTKDGKLPPESVIADFDSRFANTRTGALAKLLRGRIRLENGDADGAAEILNS
                     NVFKQKTNLGDYALWLRGKALLQARKPAEAMSVFQQLADEFPNSLRVKEAKLLWTEAA
                     LQSGMAQNVPNFLRDLVDKNDASAFLAVARAYEQQQNQAQAIGFYRKAYFYGAGSEAG
                     KQAETKLSELAQGLTPQTADEAISRADRLYDAKNYSDASAAYTAALAGFPNLSSPQTN
                     LRRLTALAILRRGAEAQGAFNLIPASAREKEEAYYQLARAYAGARQWQQARQITDEMR
                     QKFPSGIWTPKALIAVGMAARDAKNKLEETYFLQSAVTSYPNAVEVAPAQFELAWLEH
                     EGNNFAVSAQMLTEHLARHANKDTTNRGKAGYWSARDSERAGKIEEACALYEAINHRY
                     GANWYGYMATQRIASLRSQGRCNSPQNFAKDSLVGRAVANLKTVTVAAETAAEKENER
                     LVRAEQLGIVGLFDWAFVEVGEAARTAPNSPKVNLTLAKYYRLREDNVNALLSLARSY
                     PDYPQMFPEEMGREEWDIFYPLTNWQHIKMWAQARRLDPFQVAGLIRQESVFNPRAKS
                     GANAYGLMQLLIPTARATARKYGNTTTITGESLFQPALNIELGTAYMRDQFDKFGRIE
                     YVAIAYNAGPGRVAPWRASLPIELDDFVE"
     gene            2269..>2630
                     /locus_tag="VGB68_09590"
     CDS             2269..>2630
                     /locus_tag="VGB68_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049525.1"
                     /translation="MKRLFASLLFVFLLALAAQAQTPKPTAAPTPKPVADDEDVVKIS
                     TTLIQLDVTVTDKSGKVVTDLKPEDFEIYENGEKQEITNFSFISSAPAVKPPANATTG
                     GKNTTPPLPPTELKPERIR"
BASE COUNT          563 a          741 c          705 g          621 t
ORIGIN      
        1 cttcgacaaa atcgtccagc tctatcggca agctggcgcg ccacggcgcg acgcgtccgg
       61 gtccggcgtt gtaggcgatg gcgacgtatt cgatgcgccc gaatttatcg aactggtcgc
      121 gcatataagc cgtgccgagt tcgatattga gagcgggctg aaaaagcgat tcgcccgtaa
      181 tggtcgtcgt atttccgtat tttcgcgccg tcgcgcgagc ggtcggaata agcagctgca
      241 tcaagccgta ggcatttgcg ccggatttgg cgcgcggatt aaaaaccgat tcctggcgaa
      301 tcagaccggc gacctgaaac gggtcgagcc gccgcgcctg cgcccacatc tttatatgct
      361 gccagttggt caaaggataa aaaatatccc attcctcgcg tcccatttct tcgggaaaca
      421 tctgcggata atccgggtag ctgcgcgcta aagataataa agcgttgacg ttatcttcgc
      481 gcaggcgata atattttgcc agcgtcagat tgactttcgg gctgttcggc gcggttcgcg
      541 cggcttcgcc gacttcgaca aacgcccagt cgaaaagccc gacgatgccg agctgctcgg
      601 cgcgaacgag acgttcattt tctttttccg cagcggtttc ggcagcgacc gtcacggttt
      661 tcagattggc gacggcacgc ccgaccagcg aatctttcgc aaaattctgc ggcgaattgc
      721 atctgccctg cgagcgcaga ctggcgattc tctgcgtcgc catatagccg taccagtttg
      781 cgccgtagcg atgattaatc gcttcgtaaa gcgcgcacgc ctcctcgatt tttccggcgc
      841 gttcggaatc gcgcgccgac cagtaaccgg ctttgccgcg attggtcgtg tctttattcg
      901 catggcgcgc cagatgctcg gtcagcattt gcgccgaaac ggcgaaatta ttgccttcgt
      961 gttccagcca ggcgagctcg aactgcgccg gagcaacctc gaccgcgttc ggataagacg
     1021 ttaccgccga ctgcaaaaaa taagtttctt ccaatttatt ttttgcgtcg cgcgccgcca
     1081 ttccgacggc aatcaaagct ttcggcgtcc agatgccgga cggaaatttt tggcgcattt
     1141 catccgtaat ttgtcgcgcc tgctgccatt ggcgcgcgcc ggcataagcg cgggcgagct
     1201 ggtaataagc ttcttctttt tctctcgccg acgcgggaat taaattaaac gcgccctgcg
     1261 cttccgcgcc gcgccgcaga attgccagcg ccgtcaagcg ccgcagattc gtctgcggcg
     1321 acgagagatt cggaaagcct gccagcgcag ccgtgtaggc ggcagacgca tcgctgtagt
     1381 ttttcgcgtc gtaaagcctg tcggcgcgtg aaatggcttc gtctgccgtc tgcggcgtta
     1441 agccttgcgc cagctcgctt aatttcgttt cggcttgctt gccggcttcc gagcctgcgc
     1501 cgtagaaata agcttttcga taaaaaccga ttgcctgcgc ctgattttgc tgctgctcgt
     1561 aagctctggc gactgccaga aacgcgctcg cgtcgttttt atcgactaaa tcccgcaaaa
     1621 aattcgggac gttctgcgcc attcccgatt gcagagccgc ttccgtccag agcagttttg
     1681 cttctttgac gcgcagggaa ttcggaaact cgtcggcgag ctgctgaaaa acgctcatcg
     1741 cttcagccgg tttccgcgcc tgcagcagcg ccttgccgcg cagccacaaa gcataatcgc
     1801 ccagattcgt tttctgcttg aagacgtttg agtttaaaat ctcagccgcg ccgtcggcat
     1861 cgccgttttc gagccgaatc cgaccgcgca gaagctttgc cagcgcgccc gtgcgcgtgt
     1921 tggcgaaacg cgaatcgaaa tcggcgataa ccgattcggg cggcagcttg ccgtctttcg
     1981 tcaattgccg aatcgcctgc aatgcctgtt cttcggtcat ccgcgaaacc atcggctggc
     2041 agccgacatt aaacgatacg acgtgcagca cgagcgaaac taaaatgaaa ctgaaaaacg
     2101 agtgtgaatt tagctgaaaa actttgtgaa tcaatgtttt atttcctttc ttcaaacgtc
     2161 tttgatgcga aatgcgattt tgtagtttat atcggaatta atcttttgcg cagggcaatt
     2221 ttaatatatg ataaccgcat caaatggatt ttttctagga gtttggtcat gaaaaggctt
     2281 tttgcttcgc tattgttcgt ttttctcctt gcgctggcgg ctcaggcgca gacgccgaaa
     2341 ccgaccgccg cgccgacgcc gaagccggtc gccgacgacg aagacgtggt aaaaatttcc
     2401 acgacgctca tccagcttga cgtgaccgtg acggataaat cgggaaaagt cgtgacggat
     2461 ttgaaacccg aagattttga gatttacgaa aacggcgaaa aacaggaaat cacgaatttt
     2521 tcttttattt cctcggcgcc ggcagtaaag ccgccagcca atgcgacgac cggcggcaaa
     2581 aacacaacgc cgccgctgcc gccgaccgag ctaaagccgg aaagaatccg
//
LOCUS       DASWNE010000484         2643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_85932, whole genome shotgun sequence.
ACCESSION   DASWNE010000484 DASWNE010000000
VERSION     DASWNE010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85932"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1990
                     /locus_tag="VGB68_09595"
     CDS             <1..1990
                     /locus_tag="VGB68_09595"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF008907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEY0049526.1"
                     /translation="IGHRQTKNGKDVKIQLASNPSHLEFVDPVVEGMVRARQDELLET
                     GAERDAVHDKILPVLLHGDAAFAGQGIVMETLQLANLKGYRTGGTIHIVINNQIGFTT
                     SPESSRSSIYSTDAAQITQLPIFHINGDDPEAAYRTVRIALDYRQEFNKDVALDLIGF
                     RRLGHNEGDEPTYTQPLMYAKVKAHPGVRHLYAQQLIREGVISENDLNEMTSRVVSKY
                     EEILRRAKQIAADKQEKTTLKPAAAEEDGSRLFETGVSREILQTVSNKISLVPEGFNV
                     NPKMVGQLARRAKMGAGSVPMDWGFAEALAFGSLVLEGNFVRLSGQDSGRGTFSQRHA
                     LMYDTQTGAKWSPLGELRSDKNPRARFHVFDSSLSEQGVLGFEYGYSTVAQDAFVAWE
                     AQFGDFANGAQVIIDQYIAASEDKWGQKCRLTMLLPHGYEGQGPEHSSARLERFLQLC
                     AENNLQVCYPTTPAQYFHLLRRQVHQEIVRALIVMTPKSLLRLPAAVSTIEELETGGF
                     QPVIDDAKVKDKAKVKRIVLCSGKVFYDLDTARADADDDRVAIVRLEQFYPFPAEKLK
                     EVFAAYPNATQLFWTQEEPQNMGGWLFVEQRLEQIKGNLSLRYVGRTASASPATGSYA
                     IHNLEQEKLVNESLIEDSTEISAASTPEVSRQIAPASS"
     gene            2093..2419
                     /locus_tag="VGB68_09600"
     CDS             2093..2419
                     /locus_tag="VGB68_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049527.1"
                     /translation="MTKREIVWLIVKLIGVYFLYEAATSALGLSGTIFTLISVADRHL
                     RDDDKLNFFKVIAWAIGTTFFYGWIGWYLIKDGRLLFAILNREEPPPVFKDLKKEPID
                     FSDKQN"
     gene            2458..>2643
                     /locus_tag="VGB68_09605"
     CDS             2458..>2643
                     /locus_tag="VGB68_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049528.1"
                     /translation="MLRQKVLFILFSVTLLSFSIIASSCQTRDIRVPTASAGQVEPQP
                     TPATSREPITIAAGGDIM"
BASE COUNT          625 a          693 c          736 g          589 t
ORIGIN      
        1 aattggacat cgccagacga aaaacggcaa agacgtaaaa atccaactcg ccagcaatcc
       61 ttctcacctg gaattcgtcg atccggtggt cgaaggaatg gtgcgcgcca gacaagatga
      121 attattggaa accggcgcgg agcgcgatgc ggttcacgat aaaattctgc ctgttttgct
      181 gcacggcgac gcggcttttg ccggacaggg catcgtaatg gaaactttgc agctggcgaa
      241 tctgaaaggc tatcggacgg gcggcacgat tcacatcgtc atcaacaacc aaatcggttt
      301 tacgacctcg cccgaatctt cgcgcagctc gatttattcg accgacgcgg cgcagattac
      361 gcagcttccg atttttcaca ttaacggcga cgaccccgaa gcggcttacc gcacggttag
      421 aatcgcgctc gattaccgac aggaattcaa caaggacgtg gcgctcgacc tgatcggctt
      481 tcgccgtctg gggcataacg agggcgacga gccgacctac acgcagccgt tgatgtatgc
      541 caaggtcaaa gcgcatccgg gcgtgcgaca tctctacgcg cagcagctta tccgcgaagg
      601 cgtcatttcg gaaaacgatt tgaacgaaat gaccagccgc gtcgtgtcga aatacgagga
      661 aattctgcgc cgcgccaagc aaatcgctgc cgataaacag gaaaaaacca cgctcaaacc
      721 ggcggcggct gaagaagacg gctcgcgtct tttcgaaacg ggcgtctcgc gtgaaatcct
      781 gcagacggtt tcaaacaaaa tctcgctcgt tcccgaaggc tttaacgtca atccgaaaat
      841 ggtcgggcag cttgcccgcc gcgcgaaaat gggcgctggg agcgtgccga tggattgggg
      901 ctttgccgaa gctctggcgt tcggctcgct ggttctggaa ggaaattttg tgcggctgag
      961 cggtcaggat tccggacgcg gaacgttttc gcagcgtcac gcgttgatgt acgacacgca
     1021 gaccggcgcg aaatggtcgc cgctcggcga gctgcgcagc gataaaaatc cgcgcgctcg
     1081 tttccacgtt ttcgacagct cgctttccga acagggcgtg ctcggttttg aatacggtta
     1141 ttcgaccgtc gcgcaggacg ctttcgtggc atgggaagcg cagttcggcg atttcgccaa
     1201 cggcgcgcag gtgattatcg accagtacat cgccgcttcg gaagacaaat ggggacagaa
     1261 atgccgcctg acaatgcttc tgccgcacgg ctacgaaggt cagggaccgg aacattcttc
     1321 ggcgcgtctg gaaagatttc tccagctttg cgcggaaaac aatctgcagg tttgttatcc
     1381 gacgactccg gcgcagtatt tccatctgct tcgccggcag gtgcatcagg aaatcgtgcg
     1441 ggctttgatt gtgatgacgc cgaaaagtct tctgcggctt ccggcggcgg tttcgacgat
     1501 tgaagaactg gaaacgggcg gttttcagcc ggtcatcgac gacgcgaaag tcaaagacaa
     1561 agcgaaggtc aaacgcatcg ttttgtgttc gggcaaagtc ttctacgatt tagacacggc
     1621 gcgcgccgat gcggacgacg atcgcgtggc gattgtccgg ctcgaacagt tttacccgtt
     1681 tccggcagaa aagctgaaag aagttttcgc cgcttacccg aatgcgacgc agcttttctg
     1741 gacgcaggaa gaaccgcaga atatgggcgg atggcttttc gtcgagcagc ggcttgagca
     1801 aatcaaggga aatctgagcc tgcgttatgt cgggcgcacg gcttcggctt cgcccgcgac
     1861 cggctcgtat gcgattcaca atctggaaca ggaaaaactc gtcaacgaat cgttgattga
     1921 agattcgacc gaaatctcgg cggcgagcac gccggaagtt tcgcggcaaa tcgctccggc
     1981 gagttcctga aacagaggct ttaccacctt gcgaaaaggg cgaaacgtga ttacaatacg
     2041 tttcgccctt tttttattca ctcactgtat tcgtaagcgg agattgcgag aaatgacaaa
     2101 gcgtgaaatc gtctggctta tcgtcaaatt aatcggcgtg tattttctct acgaagctgc
     2161 aacgtcggct ctcggtctga gcggaacgat ttttacttta atatcggttg ccgaccgtca
     2221 cctccgcgac gacgataaac tcaatttttt caaggttatc gcgtgggcga ttgggacgac
     2281 gtttttttac ggctggatcg gctggtatct gattaaggac ggcagacttt tatttgccat
     2341 cctgaaccgc gaagaaccgc cgcccgtgtt caaggattta aaaaaagagc cgattgattt
     2401 ttccgataag caaaattaat aaaaatcagc atcttagatt cagcggtttt tccatctatg
     2461 ttaagacaaa aagttttatt tattttattc agcgtcacgc ttctgagctt ttccataatc
     2521 gcctcttcgt gccagaccag agacattcgc gtcccgaccg ccagcgccgg acaggttgag
     2581 cctcagccga cgccggcgac cagccgcgag ccgataacga ttgcggcggg cggcgacatt
     2641 atg
//
LOCUS       DASWNE010000485         1853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_157688, whole genome shotgun sequence.
ACCESSION   DASWNE010000485 DASWNE010000000
VERSION     DASWNE010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157688"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="VGB68_09610"
     CDS             complement(<1..798)
                     /locus_tag="VGB68_09610"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0049529.1"
                     /translation="MSNTNRQKRFCLVLVKPSHYDDDGYIIQWLRSAIPSNSLAVLYG
                     LAKECDENKALGDDVKLEIHAFDETNTNIRTKHIISLIENADAGMVMLVGVQSNQFPR
                     ALDIARPLRERNIQVAVGGFHVSGTISMLREIDPDVKKAQEMGVSIFAGEAEGRLEQV
                     LRDAAAANLQPLYNFMDDLPNIEGVATPLLPAERVHLTAGATTSFDAGRGCPFACSFC
                     TIINVQGRKSRRRSPADIERIVRENVKQGLHSFFITDDNFARNKDWES"
     gene            complement(916..1674)
                     /locus_tag="VGB68_09615"
     CDS             complement(916..1674)
                     /locus_tag="VGB68_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049530.1"
                     /translation="MNFKTLILFLSLSLFTFSCAQPERHQNHRQPAENQKAETISADA
                     PVADSEIRADPHMRMTRMRQPRAEDKARAAEIAEKARRSIEKYKDYKLALRDGFEILL
                     PEVPQNMYHFNKLANYMEAESRFDPQRPTSLLYEKTADGYRLIGVMYTAPAGMPEDDL
                     NERVPLSVTQWHEHVNICLPKGSDTFKGLIGQGDKFGLAGSITTREECGAAGGQFLPR
                     LLGWMVHLYPYEPTVEQMWAVERQMNSGDKSHAH"
BASE COUNT          411 a          467 c          495 g          480 t
ORIGIN      
        1 cgattcccag tccttattgc gggcgaaatt atcgtcggtg ataaaaaagg aatgaagacc
       61 ctgtttgacg ttttcgcgca cgatgcgctc gatgtcggcg ggcgagcggc gacgcgattt
      121 ccgtccctgg acgttgatga tggtgcaaaa ggaacaggca aaaggacagc ctcgaccggc
      181 atcgaaactg gtcgtcgcgc cagccgtcag atgaactctt tccgccggaa gcaagggcgt
      241 cgccacgcct tcgatgttcg gcaaatcgtc cataaaatta tagagcggct ggagattcgc
      301 cgctgccgcg tcgcgcagaa cctgttcgag ccgaccttcg gcttcgcccg caaatatgga
      361 aacgcccatt tcctgcgctt ttttaacgtc cgggtcgatt tccctgagca tcgagattgt
      421 gccggagacg tgaaaaccgc cgaccgccac ctgaatattt cgctcgcgca gtggtcgcgc
      481 aatatcgagc gcgcgcggaa actgatttga ctggacgccg acgagcatca ccattccggc
      541 gtcggcattt tcgatgagcg agattatatg tttcgtgcga atgttcgtat tcgtttcgtc
      601 aaaagcgtga atttccagct ttacatcgtc gcccagagct ttgttttcat cgcactcttt
      661 ggctaaaccg tagagaaccg ccagcgaatt tgagggaatc gccgagcgca gccattgaat
      721 aatatagccg tcgtcatcgt aatgcgacgg tttgacgagg acgagacaga aacgtttttg
      781 ccggtttgta ttgctcattg gtgtcggaat cggacgcttt tctttaataa gaataagcgt
      841 tgaattatta cattcggacg acgaaaactc aaatttggcg cgaaaattat tgccgtgcgg
      901 cggcagtcga ttgttttaat gcgcgtggct tttgtcgccg gaattcatct gtctttcgac
      961 cgcccacatc tgttcgaccg tcggctcgta aggataaaga tgcaccatcc atccgagcag
     1021 gcgcgggaga aattgcccgc ccgccgcgcc gcattcttcc ctggtcgtaa tcgagcctgc
     1081 cagaccgaat ttgtcgccct gaccgattaa gcctttgaag gtgtcgctgc ctttcggcag
     1141 gcagatgttg acgtgctcgt gccactgcgt cacgctcagc ggaactcttt cgttcaaatc
     1201 gtcttccggc attccggcgg gcgcggtata catcacgccg attaaacgat aaccgtcggc
     1261 ggttttttca taaagcagag aggtcgggcg ctgcgggtcg aagcggcttt cggcttccat
     1321 ataattcgcc agcttgttga aatgatacat attctgcgga acttcgggca gaaggatttc
     1381 gaatccgtcg cgcagggcga gcttgtagtc cttatacttt tcaatcgagc gccgagcctt
     1441 ttcggcaatc tccgcggcgc gcgctttgtc ttcggctcgc ggctggcgca ttcgcgtcat
     1501 tcgcatatgc gggtcggcgc ggatttcgga atcggcgacc ggcgcatcgg cggaaatcgt
     1561 ttccgctttt tgattttccg ccggttggcg atggttttga tgcctttcgg gctgggcgca
     1621 ggaaaacgta aaaagcgata gcgagaggaa taaaatcaac gttttaaaat tcattttcag
     1681 cttatttatc atgattgaag actttgtcag gtaaaacccc gaaaaatata atttccttga
     1741 aaactaacgt gatttcatta gattgaattt tcagccttgc ctgttgtccg tataaaactc
     1801 gcgaaatgag gcttcggaga aaaatttcga ggattttaga gcgaatattt ctt
//
LOCUS       DASWNE010000486         3299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_612808, whole genome shotgun sequence.
ACCESSION   DASWNE010000486 DASWNE010000000
VERSION     DASWNE010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612808"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VGB68_09620"
     CDS             <1..733
                     /locus_tag="VGB68_09620"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY0049531.1"
                     /translation="TVYRAENGVSDDVIYHLHGRNLDASVWNDDTYFTSQLQAFWQRL
                     SIKPPTVVVVSYGPVWLLTPKNSREESGLLDDFINRLPEIESKIGKPKRRILLGESMG
                     GLNVLIAGLSRPEMFSKVASLCPGVYLESPLSDYSTMRAGIKRTGADPKIIYGVTQLA
                     KRYVSSSEEWKAISPISLIERADQNYPELYLSCGLYDRYGNFEGTEALADIALKKGVK
                     TVWHPLYGGHCAIDIASLAGFLVAD"
     gene            1206..>3299
                     /locus_tag="VGB68_09625"
     CDS             1206..>3299
                     /locus_tag="VGB68_09625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049532.1"
                     /translation="MLFLAVPMPVKAAGEIDTSFNAGVFRQPRGSATTVVAQPDGKVL
                     IGGNFEVASGALRYSLVRVNTDGSADTSFNPPPLTDNSGGDAPGSVEAIALQADGKII
                     VAGSFRITGLSLEAPIVRLNADGSLDASFFASGASGVIKDVEIRADGKIFIAGSFNYT
                     TPPSVRRANLARLNADGSPDETFNPPGVSLPIDDIAVQPDGKVIGFAAPAAPDMGGRF
                     LRRLNEDGSPDTFFNTWVSGDVFSIKIQPDGKIIIGGWFGAANNTLRYIIARLNTDGS
                     LDPTFNNPFQSQTNQVIYEIEIQSDGRILIGGTHYSSGGPAGGFSMRFNADGTGVQYF
                     NARPPNNGAYIQVFDFALQSNGQFLLVGEMTPPEFPNQPFSGNLGAAANRINADGSYD
                     TTFQTPIGIRGNVRDIAPLPNGQIVITGLFSAVNQTIRHARIARLNPDGSTDPTFINS
                     TFDSDSNALVLAVTPLADNRVFIGGWFYGGGLERLNPDGSGDPTFTPQLLSSSIIYDI
                     AVQPDGKVIVAGGVSQDNSTCCLMRFNPDGSLDSSFRFPVGITAVRKVLVQPDGKILI
                     GGASSGGNFARRSVARLNADGSLDTSFTPVATNNTVYNFAHEMVFDIALETDGKVLIS
                     GEFSRVNGVGRQGIARLNANGSLDTSFNAAANSSVSTVKLQADGKILVGGAFTSIGGA
                     ARNRIARLNPNGSIDS"
BASE COUNT          776 a          860 c          885 g          778 t
ORIGIN      
        1 caccgtttac cgcgccgaaa acggcgtcag cgacgacgtc atttatcatt tacacggaag
       61 aaatttagac gcttccgttt ggaacgacga cacttatttc acctcgcagc tgcaagcctt
      121 ttggcagaga ttatcgatca agccgccgac cgtcgtggtc gtctcctacg gaccggtctg
      181 gctgctgact ccgaagaatt ctcgtgagga aagcggcttg ctggatgatt ttatcaaccg
      241 cctgccggaa atcgaatcga aaataggcaa accgaaacgc cgaatcctgc tcggcgaatc
      301 gatgggcggc ttaaatgtcc tgatcgccgg gctgagccgc cccgaaatgt tttctaaagt
      361 ggcgtctctt tgtccgggtg tttacctgga atctccgctt tctgattatt ccactatgag
      421 agcgggtatc aagcgtacgg gcgctgatcc caaaatcata tacggagtga cgcaattggc
      481 gaagcgatat gtttcgagca gtgaagagtg gaaagcaatc tctccgattt cattgatcga
      541 gcgcgccgat caaaattatc cggaactcta tctttcgtgc ggtctgtacg acaggtacgg
      601 aaatttcgag ggcaccgaag cgctcgccga tatcgcgttg aaaaaaggag taaagaccgt
      661 ctggcatccg ctttacggcg ggcattgcgc tatcgatatt gcgtcgctcg ccggctttct
      721 cgtagccgat taacttgtta tagattgtga aatatggtga aagccgtgtt tcttatcgta
      781 aattttcaca tttgcacggg aaacggcttt gaatgtttgc tgttttattc gctaattcaa
      841 ttatccatca caaaaactat tgacactcat caggaaataa actacattca ccttaaccga
      901 cgaccaatta aatccgatta gcatttgagg ggaaatgcta aaaccggaga atttgcggat
      961 tttattattt gcagattttc gggtgcggat ttaatgagtc ggtttttgtt tgaaacggaa
     1021 ctcttaaaca actaaatctt caccatttcg aggctgttta agcaaaagat gatgaagcgc
     1081 gggaaaagta tcgccgaata aattacttca ggtgatttga attgtgaaaa ggagtgttcg
     1141 agatgcctac gacgctaata aaaatatttt tcagaactca tttctcaatc ctttcgacag
     1201 ttttaatgct ttttttggca gtgccgatgc cggtaaaagc ggcgggagaa atcgacacga
     1261 gctttaacgc cggagttttc aggcagccga gaggcagcgc gacgacggtt gtcgcacagc
     1321 cggacgggaa agttttaatc ggcggaaatt tcgaggtcgc gagcggcgct ctcagatatt
     1381 ctctggttcg cgtcaatacg gacggaagcg ccgacacttc gtttaatccg ccgccgctga
     1441 ctgataattc gggcggcgac gctccgggca gcgtcgaggc aatcgccctg caggcggacg
     1501 gcaaaatcat cgtcgccgga agctttagaa ttaccggctt gagcctcgaa gcgccgattg
     1561 tgcgcctgaa cgcggacgga tcgctcgacg cgtctttttt tgcgtcgggc gcgagcggcg
     1621 ttatcaaaga cgtggaaatc cgcgcggacg gcaaaatttt tattgccgga agcttcaatt
     1681 atacgacgcc gccgagcgtc agaagagcca atctggcgcg gttaaacgcg gacggctcgc
     1741 ccgacgagac gtttaatccg cccggcgttt cgcttccgat tgacgacatc gccgtgcagc
     1801 ctgacggcaa agtcatcggt tttgccgcgc ccgccgcgcc cgatatgggc ggaagatttt
     1861 tgaggcggct caacgaggac ggctccccgg atactttctt taacacatgg gtcagcggcg
     1921 acgttttttc catcaaaatt cagccggacg gaaaaatcat catcggtgga tggtttggcg
     1981 cggccaataa tactttgcgt tatataatcg cccgcttgaa cacggacggc tcgctcgacc
     2041 cgactttcaa caaccccttc cagtcgcaga cgaatcaggt gatttatgaa atcgaaatcc
     2101 aaagcgacgg caggatttta atcggcggca cgcattacag cagcggcggt cccgccggcg
     2161 gtttttctat gcgattcaac gccgacggaa ccggcgtgca gtatttcaat gcccgtccgc
     2221 caaacaacgg agcttatatc caggtctttg actttgcgct ccagtcgaac ggacaattcc
     2281 tgcttgttgg cgaaatgacc ccacccgaat ttccgaatca gccgttcagc ggaaatttag
     2341 gcgcggcggc aaatcgaata aacgccgacg gctcatacga caccactttt caaacaccga
     2401 tagggattcg gggaaatgtc cgggacattg cgccattgcc gaacgggcaa atcgtcatca
     2461 ccggactttt cagcgcggtc aaccagacga ttcgccatgc gagaatcgcg cgcctcaacc
     2521 cagacggcag caccgacccg acgtttatta actcaacgtt tgacagcgac tctaatgccc
     2581 tcgttctggc ggtgacgccg ctggcggaca accgggtttt catcggcggc tggttttatg
     2641 gcggcgggct ggagcggctg aatccggacg gctcgggcga cccgactttt actccacagc
     2701 ttttaagctc gtctattatc tacgacatcg cggtacagcc ggacggaaaa gtaatcgttg
     2761 ccggcggagt atcgcaggac aattctacgt gttgtctgat gaggtttaac ccggacggct
     2821 cgctcgattc ttcgttccgg tttcccgtcg gaatcacggc agtcagaaaa gttctggttc
     2881 agccggacgg gaaaattctg attggcggag cgtcttccgg cggcaatttt gcgcgccgca
     2941 gcgtcgcgcg attaaacgcc gacgggtcgc tcgatacgtc gtttactccg gtcgcaacga
     3001 acaataccgt ttacaacttc gcgcacgaaa tggttttcga catcgcgctc gaaacggacg
     3061 gaaaagtcct gatcagcggc gagttctcaa gggtcaacgg tgtcggcaga caaggtatcg
     3121 cccgtctgaa cgcgaacggt tcgctcgaca cttcctttaa cgcggcggcg aattcgtcgg
     3181 tttccacggt caagcttcag gcggacggaa aaattctggt cggcggcgcg tttaccagta
     3241 tcggcggcgc ggcgcgaaac cgcatcgctc gtctgaatcc gaacggctca atcgattcg
//
LOCUS       DASWNE010000487         2393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_686863, whole genome shotgun sequence.
ACCESSION   DASWNE010000487 DASWNE010000000
VERSION     DASWNE010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686863"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1313)
                     /locus_tag="VGB68_09630"
     CDS             complement(<1..1313)
                     /locus_tag="VGB68_09630"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0049533.1"
                     /translation="MPDLIIKYSDRPDDKFRLARLRTTIGRSARSDICIPDPFASRLH
                     AEIRQEGDSYWLHDLGSANGTRYNGSLVSMPVPMLSGGEIQIGETVIVFQDGRSSTGK
                     SATLISDSTQALNPAMTIALSPLKSATAEILEAQPGGSTRNGLLTLISKVGIALLGST
                     SFEETLQMLASLVFEAVPAERCVIMLRDEDNPDKLKIEVAKMRGSEEALQEVRISRTV
                     MEEVTKNGKSVLTSDAQHDPRYASQTMALLGIRSVLAVPLSVGTEVFGLIYADSPTYE
                     TSFTEEHLNILTTLASVASIRVENARLTEERFERERLEHELKLASEIQQRLQPSSPPI
                     VEGYDLQGVSFSCYEVGGDYYDFIPRCDGKLLVALGDVSGKGTAAALLMSSLHAAMHA
                     QVAANSSLLETINAVNQYLAKNTPTNRFVTLFLAELDAETGSMYF"
     gene            complement(1418..1957)
                     /locus_tag="VGB68_09635"
     CDS             complement(1418..1957)
                     /locus_tag="VGB68_09635"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY0049534.1"
                     /translation="MSARVERASAPTTPSIRTRTEEVIVNFDAARVKAPFLLRCGALL
                     IDYIILVSLPVLGLLIARLSGDDGAKLFKSAFNSAGWLIAVLLAVTNFIIFPMLGGQS
                     IGKMFTGLRIIRADGQAASFSQLLLRHLAGYPLTALTFGLGFLFSVFSAKGRALHDLV
                     AGTVVVYGQKKPKVLENSK"
     gene            complement(2008..>2393)
                     /locus_tag="VGB68_09640"
     CDS             complement(2008..>2393)
                     /locus_tag="VGB68_09640"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0049535.1"
                     /translation="SAQKPVFVAAAPRLTARLFIVDTSELEPPAFNLEKDTSLIGRRD
                     PMSNIFPEVDLSKFDPQTKISRRHARIWREGDKFLIEDLGSSNGTILLTKQSDPVRLL
                     PQQPRALAHGDKLKLGDTTLHFVLG"
BASE COUNT          540 a          651 c          656 g          546 t
ORIGIN      
        1 atgaaataca tcgagcccgt ttcggcgtcg agctcggcga gaaaaagcgt gacgaaacga
       61 ttggtcggcg tgtttttcgc caggtattga ttgacggcgt taatcgtctc gagcagagaa
      121 gaattggcgg cgacctgcgc gtgcatcgcg gcgtgcaggc tcgacattaa aagcgcggcg
      181 gctgttccct tgccggaaac gtcgccgagc gcgaccagga gtttcccgtc gcagcgcgga
      241 ataaaatcgt aataatcgcc cccgacttcg tagcaggaaa acgaaactcc ctgtaaatca
      301 tagccttcga caatcggcgg cgaagacggc tgaagacgct gctgaatctc gctcgccagc
      361 ttcagctcgt gttccagacg ctcgcgctca aagcgttctt cggtcaggcg cgcgttttcc
      421 acgcgaatcg aagcgaccga agccagcgtc gtcaaaatat tcagatgttc ttccgtaaag
      481 ctcgtttcat aggtcggcga atcggcgtaa atcaagccga aaacttccgt gccgacgctc
      541 aacggaacgg cgagaaccga gcgaattccg agcagagcca tcgtctggct ggcgtaacgc
      601 gggtcgtgct gcgcgtcgga agtcaaaacg gatttgccgt ttttcgtgac ttcttccatc
      661 accgtgcggc tgatgcggac ttcctgcaga gcctcttcgc tgccgcgcat tttggcgacc
      721 tcgattttta atttgtcggg attgtcttcg tcgcgcagca ttatgacgca gcgctccgcc
      781 ggcacggctt cgaacacgag cgaggcgagc atctgcaaag tttcttcaaa gctggtcgag
      841 ccgagcagag cgatgccgac tttgctgatt aatgtcagca gaccgttgcg cgtcgagccg
      901 ccgggctgcg cttcgagtat ttccgccgtc gcgcttttca gcggcgaaag cgcaatggtc
      961 atcgccggat tcagagcctg cgtgctgtcg gaaatcaagg tcgcgctttt ccccgtcgac
     1021 gagcgcccgt cctgaaacac gatgaccgtt tctccgattt gaatctcgcc gccggaaagc
     1081 atcggaacgg gcatcgaaac cagcgagccg ttgtaacgcg tcccgtttgc cgagcccaaa
     1141 tcgtgaagcc agtagctgtc gccttcctgc cggatttccg cgtgcagccg cgaggcaaac
     1201 gggtcgggaa tacaaatgtc gctgcgcgcc gaacgcccga tggtcgtccg cagccgcgcc
     1261 agacggaatt tatcgtcagg tctatcggaa tattttatga taaggtcggg cataaattta
     1321 attacgaatt acgaattacg aattacgaat ttgaaaatag attcaagggc gtttgcggga
     1381 gaattttctt cattcgtaat tcgtaattcg taatttttca ttttgaattt tccaggactt
     1441 tcggtttttt ctgaccgtag acgacgacgg ttcccgcgac caaatcgtgc agggcgcgtc
     1501 ctttcgcgct gaaaaccgaa aataaaaaac cgagtccgaa agttagagcc gtcagcggat
     1561 aaccggcgag atgcctcagc agaagctggc tgaagctcgc cgcctgcccg tcggcgcgga
     1621 taattctcaa gccggtaaac atttttccga tgctctgacc gccgagcatc ggaaaaatta
     1681 taaaattcgt gaccgccagc aaaaccgcaa tcagccagcc cgcgctgtta aacgcgcttt
     1741 taaaaagctt tgcgccgtcg tcgccggaaa gccgcgcgat tagcaagccc aaaacgggca
     1801 ggctgaccaa aattatgtaa tcaatcagca aagcgccgca gcgcagcaaa aaaggcgctt
     1861 tgacgcgggc ggcatcgaag ttgacgatga cttcttcggt tctggtgcga atgctcggcg
     1921 ttgtcggcgc ggaggctctt tctactcttg cactcataaa atcgttggtg aatttttttt
     1981 tacagtcgca aattcctttt tattttttta tccgagcaca aagtgcaaag tcgtatcgcc
     2041 cagtttcaac ttgtcgccgt gcgctaaggc gcgcggctgc tgaggcagaa ggcgcaccgg
     2101 gtcgctctgt ttcgtcagca gaatcgttcc gtttgacgag cctaaatctt caatcagaaa
     2161 cttgtcgccc tcgcgccaga tgcgcgcgtg acgccgcgaa attttagttt gcgggtcgaa
     2221 tttggaaagg tcaacttccg ggaatatatt cgacatcggg tcgcgcctgc ctatcaggct
     2281 cgtatctttt tcgagattga aagccggcgg ctccaattcg ctggtgtcaa ctataaacag
     2341 acgcgccgtc aaacgcggcg cagcggcgac gaaaaccggc ttttgcgcgg agg
//
LOCUS       DASWNE010000488         1826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_368859, whole genome shotgun sequence.
ACCESSION   DASWNE010000488 DASWNE010000000
VERSION     DASWNE010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368859"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..>1826
                     /locus_tag="VGB68_09645"
     CDS             182..>1826
                     /locus_tag="VGB68_09645"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0049536.1"
                     /translation="MKVLSFTALVKIGYARHQTGGEDLAPFNDNLASAYALSLDGGSS
                     VSITASNADATKETGEPNHAENAGGKSVWFKFVPDATVTARINTSQNSFDTLLAVYTG
                     TGMGALTRTGYNDDCSSNCSTASTIDLMLTAGQTYYIAVDGYHNGTTAASGTFKLNIT
                     IFNAPPADNFASAYNLGTIDRGSIAGTNHLGTRESGEPAHYNEATPGAKSVWYKWTPL
                     STFAAAVELTENFESVLKIYKSDTANPTFNQLNFAALNIDSQSYNGSNYKATFLAEAG
                     KTYYIVVAGQGFQPDAGNFQLKFGLNKLRYSTDFSGLNQNASLSVFRPSNGTWYNLQS
                     INSSAPEYIQFGKNGDTPVPADYDGNGSANLAVTRSENGLKVWHVRGLFSQQWGLATD
                     KAVTGDFDRDGIADLAVIRTTAQGLAWHIRQSRNGGLRTFNFGVAGDKPVPGDFDGDG
                     ATDVALTRNTPEGKAWYFLLSGFDSAGQTYSRFTATLFGTAEDVPAVEDFDGDGKTEV
                     GFFRPSSGFWYFLRSSDGLLDYKSFGASGDLPQPGDYNGD"
BASE COUNT          432 a          503 c          502 g          389 t
ORIGIN      
        1 cttgccgctt tttattttcc gcggaagacg aaaacttgcc tacacgttcg tgtggtgatt
       61 tgcgttttca aagcgtctat tctccatctc gtcaaatacg aaaccgcaag aagcgcggca
      121 aatatttaag gagaaaacgg aaatgaaaaa gcaggcaaac ataatcgcta tatttttttt
      181 aatgaaagtt ttgagtttta cggcattggt caaaatcgga tacgcgcggc accaaaccgg
      241 cggcgaggat ttagcgcctt ttaacgacaa tctggcgagc gcctacgcgc tttctttgga
      301 cggcggcagc tccgtcagca tcacggcgtc caacgcggac gcgacgaaag agacgggcga
      361 gccgaatcac gccgagaacg cgggcggaaa atccgtctgg tttaaattcg tgcccgacgc
      421 gaccgtgacg gcgcgcatca acacgtcgca aaacagcttc gatactttgc tcgccgttta
      481 taccggaacg ggtatgggcg cgctgacgcg aaccggctat aacgacgatt gcagcagcaa
      541 ctgctcgacg gcgagcacaa tcgatttgat gctgaccgcc ggacagactt attacatcgc
      601 cgtcgacggt tatcataacg gcacgactgc cgcttccggc acttttaaat taaacatcac
      661 gatttttaac gccccgcccg ccgataattt cgccagcgcc tataatctgg gaactatcga
      721 ccgcggcagc atcgccggaa caaatcatct gggcacgcgc gagtcgggcg agccggctca
      781 ttacaacgaa gcgacgccgg gcgcaaaatc cgtctggtat aaatggacgc cgctttcgac
      841 ctttgccgcg gcggtcgagc tgaccgaaaa ttttgaatcg gtactgaaaa tttataaatc
      901 cgatacggca aacccgacat ttaaccagct caactttgcc gcgctcaata tcgattcgca
      961 gagttataac ggctcgaatt acaaagcgac gtttttagcc gaggcgggca aaacttatta
     1021 catcgtcgtc gccgggcagg gctttcagcc cgacgccggt aatttccagc tcaaattcgg
     1081 tttaaacaag ctgcgctatt ccacggattt ctccggcttg aatcaaaacg ccagcctttc
     1141 ggtttttcgc ccgtcgaacg gaacctggta taacctgcaa agcattaatt cttccgcgcc
     1201 cgaatatatt caattcggca aaaacggcga tacgccggtt ccggctgact acgacggcaa
     1261 cggcagtgct aatctggcgg tgacgcgcag cgaaaacggc ttgaaggtct ggcacgtcag
     1321 aggcttattt tctcagcaat ggggattggc gacggacaaa gccgtgacgg gcgacttcga
     1381 ccgcgacggc atcgccgacc tcgccgtaat tcggacgacg gcgcaaggtt tggcgtggca
     1441 tatccggcaa agccgaaacg gcggtttgcg gacttttaac ttcggcgtcg cgggcgacaa
     1501 gcccgtgccg ggcgatttcg acggcgacgg cgcgaccgac gttgcgctga cgcgcaatac
     1561 gccggaaggc aaagcctggt atttcctgtt gagcggattc gattccgccg ggcagactta
     1621 cagccggttt acagcgacgc tgttcggcac ggcggaagac gttccggcgg tcgaagattt
     1681 cgacggcgac ggaaaaaccg aagtcggttt tttccgcccg tcgagcggtt tctggtattt
     1741 tcttcgcagc tccgacggat tgctggatta caaatcgttc ggcgcgagcg gcgacctgcc
     1801 gcagccgggc gattataacg gcgacg
//
LOCUS       DASWNE010000489         4359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_299693, whole genome shotgun sequence.
ACCESSION   DASWNE010000489 DASWNE010000000
VERSION     DASWNE010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299693"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            399..1949
                     /locus_tag="VGB68_09650"
     CDS             399..1949
                     /locus_tag="VGB68_09650"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY0049537.1"
                     /translation="MKNPKLLNIVLILLFLFSLTIGSAAQVDVPRQTTAITYPEDELV
                     TVQFRGTTRFPRMKGDARIKRTRRNGTEIELSVSKMPRPFELGAGFATYVVWAVSPDG
                     QIDNLGEIKRRGFFEFDSKISVTTPLQTFAIIITAEPHFLVRRPSRAIMLENLTALSA
                     SGRTLATAKSVNYFGNTSDYFSDARTPEIAETDYSKTPSTLLQAKQAVALARYAGAQR
                     DAAEELQTAEALMQNAENSWKAGRDEESVDLTARQAVSAAVKAEDTALVRKEAREKRN
                     EKSRNDAELQQAEDKYLRAQDEITDLKAELAREQRQRELSERDSSNFNEQIRALREEN
                     GRLRDEIAKLRADSEDAKTRLARVEGEKVAIEKRREQDERSSRLRESMPVLMQSLKPF
                     GAVRQTERGIVLTLPENYFMGIRDSNLAATADVKISNLANVLANSTDYRIVVEAHTDN
                     KGTPDELQTLTQNRAQTVLDRLAAGGVEASRIEVKGYGASLPVAPNTTNLNRGKNRRV
                     DVILVPNL"
     gene            2095..3660
                     /locus_tag="VGB68_09655"
     CDS             2095..3660
                     /locus_tag="VGB68_09655"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY0049538.1"
                     /translation="MLVKSAADELQNYLTDASNLQGGHAQKLFIPETTGEVAEIIREA
                     NRTKTPVTISGARTGIVGGAVPFGGCIVSLEKFNRIKSIEKTASGGGEAIVEAGVVLN
                     DFQKQVESKGLFYPPDPTEWSCQIGGTIATNASGSRSFKYGSTRAFVEGLTVVLPTGD
                     VVNVKRGEAFAADGEPIELKTLDGNSIRAKLPTYRQPSVRKNTSGYWTAKKLDAIDLF
                     VGSEGTLGVVTEAHLKLLPKPEGFLSGIVFFKGENDLLNFVKEARGLSYETRKTRIQN
                     SKFRIQDSESAILNSESGIPNSIDASLLEYFDAEALKFIKDKFAEVPDEMAGAIFFEQ
                     ETDEESEDALFDQWNELLEKHNAETDASWFTTAAPDREKMREFRHALPVSVNEWIVRH
                     RQRKISTDIAVPDAEFPSMLRFYKQSLEASALQYVIFGHIGDCHLHVNILPKDAAEAT
                     RAKHLYGRFIAQGIMLGGTISAEHGIGKIKSKYLYVQYGERYLSEMAELKRAFDPQGI
                     LGRDNMFDAKFLG"
     gene            3793..4167
                     /locus_tag="VGB68_09660"
     CDS             3793..4167
                     /locus_tag="VGB68_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049539.1"
                     /translation="MKDLTTKILVLIIILFAVFGCSYIDTAQKTVQDTTSDKNSATNT
                     ARETLGLKKSGIRECDELVDALAAKRKQNANAEQSWSDKAVEELVKQQLYDQLSKNDT
                     NRTAQEKTDLAQKCKMVLDYVK"
BASE COUNT         1171 a         1088 c         1196 g          904 t
ORIGIN      
        1 tccagagcac gccgtccgga taaacttccg gcgaggtata gtggtgcgcg gttccctgaa
       61 taatagcgat ttccggctgc gcctcggcga ccgcgccgcc gcagaaaaag gcgattgcga
      121 aaagcaaagc ggttctgacc gttgtccgta gcatcggctt catctccttg agttggcggg
      181 ctggattcac aaacaaatat aattcaggcg taaataaatg acaacaatga aattttcggg
      241 ttgccggaga atcgtaaaac gaaatctggt atgaacaatg agccgactcg tagtaaaatc
      301 caaaattgat gcgggctctg atatataaat tagcaactga ttactgttaa agaggcatct
      361 ttcttttcgg acaaatctct ttcggagaaa aaacattgat gaaaaatcca aaacttttga
      421 atatcgttct gatacttttg tttctatttt ctttgacgat tggctcggcg gcgcaggttg
      481 acgtgccgcg ccagacgacg gcgataactt acccggagga cgaactcgtc acggtgcagt
      541 ttcgcggcac gacgcgcttt ccgcggatga aaggcgacgc gcgcatcaag cgcacgcgcc
      601 gcaacgggac ggaaatcgag ctgtcggtat cgaaaatgcc gcgcccgttc gagctcggcg
      661 ctggctttgc gacctacgtc gtctgggcgg tttcgcccga cggtcagatt gataatctgg
      721 gcgaaatcaa gcgacgcgga ttttttgaat tcgattcgaa aatctcggtg acgacgccgc
      781 tgcaaacctt tgccataatt atcacggctg agccgcattt tctcgtgcgc cgaccgagcc
      841 gcgcgattat gctggaaaac ttaacggctt tatcggcgag cggacgcacg ctggcaaccg
      901 ccaaatccgt caattatttc ggcaacacga gcgattattt ttccgacgcg cgaacgccgg
      961 aaatcgccga aaccgattat tccaaaacgc cttcgacgct tctccaagcc aagcaagccg
     1021 tcgccctggc gagatacgcg ggcgcgcaac gggatgcggc ggaagaattg caaacggcgg
     1081 aagctttaat gcaaaacgcc gaaaactcct ggaaagccgg acgcgacgaa gaatcggtcg
     1141 atttgacggc gcgccaggct gtcagcgcgg cggttaaagc cgaagacacg gcgctggtgc
     1201 gcaaggaagc gcgcgaaaaa cgcaacgaaa aatcgcgcaa cgacgccgag ctgcaacagg
     1261 cggaagacaa atatctgcgg gcgcaggacg aaattactga tttgaaggcg gaactggcgc
     1321 gcgagcagcg ccagcgcgag ctttccgagc gcgactcgtc aaatttcaac gagcaaatcc
     1381 gggctttgcg cgaggaaaac gggcggcttc gcgacgaaat cgccaagctg cgcgccgatt
     1441 ccgaagatgc gaaaacccgt ctggcgcgcg tcgaaggcga gaaagtcgcc atcgaaaaac
     1501 gacgcgagca ggacgagcgc tcgtcgcgtc tgcgcgaaag tatgccggtt ttgatgcagt
     1561 ctttaaaacc tttcggcgcg gttcgccaaa ccgagcgcgg aatagttttg accttgccgg
     1621 aaaattattt tatgggaatc cgcgacagca atctggcggc gacggcggac gtgaaaatct
     1681 ccaatctggc gaacgttctg gcaaacagca cggattatcg catcgtcgtc gaagcacaca
     1741 ccgacaacaa gggaacgccc gacgaattgc agacgctgac acaaaaccgc gcccagacgg
     1801 ttctggacag actggcggcg ggcggtgtcg aagcgtccag aatcgaggtc aaaggctacg
     1861 gggcgagcct gccggtcgct ccgaatacga cgaatctaaa ccgcgggaaa aatcgccgcg
     1921 tcgacgtgat tctcgttccg aatctttaaa tcgcttgccg gtttaatttg cgtttgacta
     1981 aagccggcat tggtcggctt ttttcgttac cggaaaatct tttgtcccgc ctgcgaagcg
     2041 taaataaatt aacccggatt tgtttgccgg gctttacggt ttataatcgg ctttatgctg
     2101 gtgaaatcgg cagccgacga gctgcaaaat tatctgaccg acgcgagcaa cctgcagggc
     2161 gggcacgcgc agaagctttt tattcccgaa acgaccgggg aagtcgcgga aattatccgg
     2221 gaagcgaacc gtacaaaaac tccggtgacg atttccggcg cgcggacggg cattgtcggc
     2281 ggcgcggtgc cgttcggcgg ctgcatcgtt tcgctcgaaa aatttaaccg gataaaatca
     2341 atcgaaaaaa cggcgagcgg cggcggcgaa gcgattgtcg aggcgggcgt cgtgctcaac
     2401 gattttcaga agcaggtcga atcgaaaggg cttttttatc cgcccgaccc gaccgagtgg
     2461 tcttgccaaa tcggcggcac gattgcgacc aacgcctcgg gctcgcgcag ttttaaatac
     2521 ggctcgacgc gcgcttttgt cgaaggcttg accgtagttt tgccgaccgg cgacgtcgtc
     2581 aacgtcaagc gcggcgaagc gtttgcggcg gacggcgagc caatcgaatt aaaaacgctg
     2641 gacggaaact caattcgcgc caaattgccg acttaccgcc agccgtcggt tcgcaaaaat
     2701 acttcgggtt actggacggc gaaaaagctc gacgcgattg atttgttcgt cggcagcgaa
     2761 ggaactttag gcgtcgtcac cgaggcgcat ctgaaattat tgccgaaacc cgaaggcttt
     2821 ctgagcggaa tcgttttttt taaaggcgaa aacgatttat taaatttcgt caaagaggcg
     2881 cgcgggcttt cgtacgaaac gcgaaagact cgaattcaaa attcaaaatt caggattcag
     2941 gattcggagt cggcaatttt gaattccgaa tccggaatcc cgaattccat cgacgcttct
     3001 ctgctcgaat atttcgacgc cgaggcttta aaatttatca aagataaatt cgccgaagtt
     3061 ccggacgaga tggcgggcgc gattttcttc gagcaggaga cggacgaaga aagcgaggac
     3121 gcgcttttcg accaatggaa cgagctgctg gaaaaacaca acgcggaaac ggacgcctcc
     3181 tggtttacga ccgccgcgcc cgaccgcgaa aaaatgcgcg agtttcgaca cgcactgccg
     3241 gtttcggtca acgaatggat tgtccgccac cggcagcgaa aaatctcgac cgatattgcc
     3301 gtgccggacg cggaatttcc gtcgatgctc aggttttata aacagtcgct cgaagcgagc
     3361 gccctgcaat acgtgatttt cggtcacatc ggcgattgtc acctgcacgt caatattctg
     3421 ccgaaagatg cggcggaagc aacgcgggca aaacatctct acgggcgttt tatcgcgcag
     3481 ggcataatgc tcggcggcac catcagcgcc gagcacggca tcggcaaaat caagagcaaa
     3541 tatctttacg tgcaatacgg cgagcgttac ctgagcgaga tggcggaact gaaacgcgcc
     3601 ttcgacccgc agggaattct cggtcgtgat aatatgtttg acgcgaagtt tttaggctag
     3661 agaagattgg gaaattacgg aaagattggg aaattacgga aagaaggaaa ttagggaaag
     3721 agggaagcga aggatttccc gtaatttccc tgatttccat aatttccgct ttcccttatt
     3781 ccacgattaa atatgaaaga cttaacaacg aaaattcttg ttttaataat tatacttttc
     3841 gccgttttcg gctgcagcta tatcgatacc gctcaaaaga cggtgcagga cacgacgtcc
     3901 gataagaaca gcgcgacgaa tacggcgcgc gagacgctcg gcttgaaaaa atccggcatc
     3961 agggaatgcg acgagctggt cgacgcgctg gcggcgaagc gcaaacaaaa cgccaacgcg
     4021 gaacaatcct ggtcggacaa agccgtcgaa gaactggtca aacaacagct ttacgaccag
     4081 ctttcgaaaa acgacacgaa ccgcacggcg caggaaaaaa ccgatttggc gcagaagtgc
     4141 aaaatggttt tggattatgt aaagtgaaaa actgataact gataactgat aactgataat
     4201 tgagaagaac caattgaaaa tagaaaattt gaaaatgtat gttttcaact atcaactatc
     4261 agttatcaac tatcaactac cagttattaa gagaggattt aaaggaagca aaaatgcggg
     4321 caattttaat ggatttcaac ggcgtgatta tcgacgacg
//
LOCUS       DASWNE010000490         3232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_86036, whole genome shotgun sequence.
ACCESSION   DASWNE010000490 DASWNE010000000
VERSION     DASWNE010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86036"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="VGB68_09665"
     CDS             <1..966
                     /locus_tag="VGB68_09665"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEY0049540.1"
                     /translation="LLNQFSLVSLPGWFFEVGEVALILAVPVAVSIFLARSVARTNRD
                     LKTQLVQVETLSAQKIEQERRSAELHAENERRANELEEARQLQISMLPKKLPELPNLE
                     IAAYMKPATEVGGDYYDFHVSEDGTLTVAVGDATGHGLKAGLVVTATKSLFNAFAGQE
                     NISQILRQTSGALKKMNLRGLFMAMAMAKIKDGEMKLSIAGMPSALIFRAASGKVEAI
                     NIRALPLGSISRFAYPEQKFSLSGGDVVLLMSDGFPEMFNESGEMLGFEKAAEVLPEI
                     AHHAPQEIIDRLVEISRRWAAERPPDDDVTFVALKVRTPESENGN"
     gene            971..3091
                     /locus_tag="VGB68_09670"
     CDS             971..3091
                     /locus_tag="VGB68_09670"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEY0049541.1"
                     /translation="MPRNAAVKNQFRYWMFSLAALLATTPFLYAQQQPEIFSLTAQSF
                     EGGKSVALEDLRWKYQAGDAAAFADKDFDDSGWKSVTNDEINDAPVEALENWDGRAWF
                     RLRLRVDESAVGKPLAARMWHWGATEIYLNGKLIQSYGVINADGSDTEYNPRGLYFPI
                     AFDSAGTHTIAVRYSFQTMSDLTSGRASWVLRGGTRPGFMMALTDARDISLRQENRAH
                     SERTDYIFIGLLWALALVHFLLFVFYRAARGNLFYSLFVLGLGLTMWLVSLGNTAHFG
                     AIPYMLRDILRINVQSLAILALLAFLYVEFRARFSRIFWILCALWAIVIVLQWMRIRV
                     DFDYTLLMLAVSIADALRIMIIALWQRRDGAWIIAAGIGFFAVGVALNITIERKFLQV
                     PQWLYNLNLYSTVLSVPLTVSLYLARNFARTNRHLEAQLVQVQELSIKQLEQERVAAE
                     LQLAHEREKAENERRAKELEEARQLQLSMLPKKLPRIAGLEIAAYMKPATEVGGDYYD
                     FHVSEDGTLTVAVGDATGHGLKAGSVVTATKSLFNAFAGEPDIARIFQKTSAALKKMN
                     LRGLFMAMAMLKIKDYSAEIGIAGMPPVLIYRSASERVEEIGIKALPLGAIAKLAYQK
                     QELQLFKGDCLLLLSDGFPEMFNGQNEMLGFEKAAEILPLIAHRTPQEIIDYFIKAGE
                     SWANGRPQDDDVTFVVVKVAETLN"
BASE COUNT          742 a          831 c          908 g          751 t
ORIGIN      
        1 ctgctcaatc aattttcgct tgtcagtctg ccgggctggt tttttgaagt cggcgaagtc
       61 gctctgattt tagccgttcc ggtcgccgtc tccatctttc tcgcccgcag cgtcgcccgc
      121 accaatcggg atttgaaaac gcagctcgtt caggttgaaa cgctttcggc gcagaaaatc
      181 gaacaggaac gccgttcggc tgagctgcac gccgaaaacg aacgccgcgc caacgaattg
      241 gaagaagcgc gtcagctgca aatatcgatg ctgcctaaaa agctgcccga gctgccgaat
      301 ctggaaatcg ccgcgtatat gaaacccgct acggaagtcg gcggcgatta ttacgatttt
      361 cacgtttccg aggacggaac tttaacggtc gccgtcggcg acgccaccgg gcacggactc
      421 aaagccggtt tggttgtcac ggcgacaaaa agtctgttta atgcttttgc ggggcaggaa
      481 aatatttcgc aaattctcag gcagacaagc ggcgctttaa aaaaaatgaa tctgcgcgga
      541 ctgtttatgg cgatggcgat ggcgaaaata aaagacggcg aaatgaagct gagcattgcc
      601 ggaatgcctt cggctttaat tttccgcgcc gcctcgggaa aggtcgaagc aatcaatatt
      661 cgcgctctgc ctttgggaag tatttcgcga ttcgcttacc cggaacaaaa attttcgctt
      721 tccggcggag acgttgtcct gctgatgagc gacggatttc ccgaaatgtt caacgagagc
      781 ggcgaaatgc tcggctttga aaaagccgcc gaagttttgc cggaaatcgc gcatcacgcg
      841 ccgcaggaaa taattgaccg gctggtcgaa atcagccgcc ggtgggctgc cgaaagaccg
      901 cccgacgacg atgtgacttt tgtggcgttg aaggtcagaa cgccggaatc tgaaaacgga
      961 aattaaagct atgccgagaa atgctgccgt caaaaatcaa tttcgttatt ggatgttttc
     1021 gctggctgcg cttttagcga cgacaccttt tctttacgcc cagcagcagc cggagatttt
     1081 ttctttgacg gcgcagagct tcgagggcgg aaaatcggtc gcgctcgaag atttgcgttg
     1141 gaagtaccag gcgggcgacg ccgcggcatt cgcggataaa gattttgacg acagcggttg
     1201 gaaaagcgtc accaacgacg aaataaacga cgcgcccgtc gaagcattgg aaaattggga
     1261 cggtcgggcg tggtttcgtc ttcgactgcg cgttgacgaa tcggcggtcg gaaagccgct
     1321 cgcggcgcgg atgtggcatt ggggcgcgac ggaaatttac ctgaacggaa agctcattca
     1381 atcttacggc gtgataaacg ctgacggcag cgacaccgaa tacaatccgc gcgggcttta
     1441 ttttccaatc gcgttcgaca gcgccgggac gcacacgatt gccgtccgtt attcgtttca
     1501 aacgatgagc gatttaacga gcgggcgggc gagctgggtc ttgcgcggcg ggacacgtcc
     1561 gggttttatg atggcgctta ccgatgcgcg cgacatctcg ctcagacagg aaaatcgcgc
     1621 ccattcggaa cgcaccgatt atattttcat cgggctgttg tgggcgctcg cgctcgtcca
     1681 ttttctgctg ttcgtttttt atcgcgccgc ccgcgggaat cttttttaca gtttgttcgt
     1741 tctcggtctc ggtctgacca tgtggctcgt cagcctcgga aacacggcgc atttcggcgc
     1801 aattccgtat atgctgcgcg acattttgcg aatcaacgtg cagtcgctgg cgattctggc
     1861 tctgctcgcg tttctctacg tcgaattcag agcgcggttt tcacgaattt tctggatttt
     1921 gtgcgcgttg tgggcaatcg tcatcgtgct gcaatggatg agaatccggg tagatttcga
     1981 ttacacgctg ctgatgctcg ccgtctccat cgccgacgct ctgcggatta tgattatcgc
     2041 cttatggcag cggcgcgacg gggcttggat tattgcggcg ggcatcggat ttttcgccgt
     2101 cggggtagcg cttaatatca ccatcgagcg aaagtttttg caggttccgc agtggcttta
     2161 caatctgaat ttatactcga cggtgctgag cgtgccgctg acggtatcgc tctatctggc
     2221 tcgaaatttc gcccgcacga accggcatct ggaagcgcag ctcgtgcagg tgcaggaatt
     2281 atccattaaa caattggagc aggagcgcgt cgccgccgag ctgcaactgg cgcacgagcg
     2341 cgaaaaagcg gaaaacgagc gccgcgccaa agagctcgaa gaagcccgcc agctgcagct
     2401 ttcaatgctg ccgaaaaaac tgccgcgaat tgccggtttg gaaatcgccg cgtatatgaa
     2461 accggcgacg gaagtcggcg gcgattatta tgattttcac gtttccgagg acggcacttt
     2521 aacggtcgcc gtcggcgacg ccaccggaca cgggctcaaa gccggttcgg tcgtcaccgc
     2581 tacgaaaagc cttttcaacg ccttcgcggg cgagcctgac atcgcgcgca ttttccaaaa
     2641 gacttccgcc gcgctgaaaa aaatgaatct gcgcggtttg tttatggcga tggcgatgct
     2701 gaaaatcaaa gattacagcg cggaaatcgg catcgccgga atgccgcccg tcttgattta
     2761 tcgaagcgcg agcgagcgag tcgaagaaat cggcatcaag gctctaccgc tcggcgcgat
     2821 agcgaaactc gcttatcaaa aacaggagtt acagctcttc aaaggcgatt gcctgttgtt
     2881 gttgagcgac ggttttcccg aaatgttcaa cgggcagaac gaaatgctcg gctttgaaaa
     2941 agccgccgaa atactgccgc tgattgccca cagaacgccg caggaaatta tcgattattt
     3001 tattaaagcg ggcgaaagct gggcaaacgg cagaccgcag gatgatgatg tgacctttgt
     3061 cgtggtcaaa gtcgccgaaa cattgaatta aaaacttcac ttttatgaaa atagctcgaa
     3121 tgaaaaatta tttcctgccg caaattgtcc ttttgtttgc aattctcgcg ctcgcacaga
     3181 ttttggctgc cgcgcagcag acgccggcaa ttgagccttt agtcattacc aa
//
LOCUS       DASWNE010000491         3570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_157773, whole genome shotgun sequence.
ACCESSION   DASWNE010000491 DASWNE010000000
VERSION     DASWNE010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157773"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3570)
                     /locus_tag="VGB68_09675"
     CDS             complement(<1..>3570)
                     /locus_tag="VGB68_09675"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY0049542.1"
                     /translation="DAQGRVTRQPEAAGFGAENGLPVKFGTAANGDIVYADIAGSRLK
                     RLVYLPGNRPPTAEATFTTNAATRTVTFDGSRSTDLDGDALTYRWDLGDGTVATGVRP
                     VKTYAATAPATVTVRLTVSDPQGAVVPGATVAVKNLDTNVVNNVTANDEGSYTVPFLL
                     PGRYSVSITAAGFKTSVRENVTVNVDDRLTIDVQLEIGQTADVDIIADTELVERGTVT
                     TGTVITQRQIEELPLSEGAAYNLATQAPGVSYTGNPQFTGPTANGNLSAIRTNGAAGN
                     QITLDGSPNLTFDGGVAYTPPADALTQFKIQTNAFDAQSGFTAGSTVNVAVKSGTNKL
                     HGSLYYFNRPEALTANNFFANRAGSERPPRQYYRYGGQVNGPVYIPKIYNGQDRTFFM
                     FSYEKQLDERAEPETFTVPTVRMRNGDFSELLPGTPIYDPATAFLGTGNNGCAASVVC
                     RTAFAGNIIPVNRLNPAAVAFLNLYPLPNQPGTFDNYFSNQSLSRPYDSYLTRIDHNF
                     NAEHRIFGKFFYSKSNEDRYNFIGEPDSITQGFEIRTNKGGSLDYTATLSSNFVLDIR
                     SSLNDFVQQRIPANPMSAADLGFNGIAAVSESTVFPRFDFTNYDTLGSERADFNEGLT
                     RNFRLFSLQPTLTQIFGNHTLKYGYDYRKLWENRSTNGYNAGRFLFTGAFTAPASNSN
                     ATTVNALGRDLASFLLGIPSANTSGSFIEQAATYDVSMNYNGFFVQDDWRVTQKLTLN
                     LGLRYELESGLREAEGRFVTGFDTTTASPLRAGALTNFNANVPASVPVAAFQNLSGGL
                     RFAENGSQSNQAIDKNNFQPRLGFSYALNDKTVIRGGFGIFTAPFQIQPINQAGFTAN
                     TTFTPSTNNGLTFLANINNPFPGGLNPAIGSSQGLNTLVGTTLGTTNATGPTAATLYT
                     YERKNANYTRFVFGIQRELPFDIGVEATFVYSRGANLPVLRQLNYIPREYLNNFENAT
                     DGGTILNTIAATQTFLNATVPNPFRGLVPSIGTLNGNTIQRRFLLTQFPQFQDVIVTE
                     YNGSNDYRSLQLQATKRLSAGFSFNASYTYAHEREKTRRLNPQDEELTDMLSVFSRPH
                     RITFSGIYELPFGRGRQFFGETHPVFDAIFGGWQFNAVYEWQSGEPLVLPNVFFDGDP
                     SSLENLLGEKDDAGRRYGIDIPAFAVGGFRVLDNRPTVNNQTNP"
BASE COUNT          754 a          960 c         1077 g          779 t
ORIGIN      
        1 gggattcgtc tggttattga cagtcggtcg attatcgagc acgcggaaac cgccgacggc
       61 gaatgccgga atatcgattc cgtagcgtct gccggcatcg tccttttcgc cgagcagatt
      121 ttccagagag ctcgggtcgc cgtcgaaaaa gacgttcggc agaacgagcg gctcgccgga
      181 ctgccattcg taaacggcgt tgaattgcca gccgccgaaa attgcgtcaa acaccgggtg
      241 cgtctcgccg aaaaactgtc gcccgcgccc aaacggaagc tcgtaaattc ccgaaaacgt
      301 aatgcggtgc gggcggctga agaccgaaag catatcggtc aattcttcgt cctgcggatt
      361 caggcggcgc gttttctcac gctcgtgcgc ataagtgtaa gaagcgttaa acgaaaatcc
      421 ggcgctcaaa cgtttggtcg cctgcaattg cagcgagcga taatcgttgc tcccgttata
      481 ttcggtgaca atcacatcct gaaactgcgg aaactgcgtc aacagaaagc ggcgctgaat
      541 cgtgtttccg tttaaagttc cgatgctcgg cactaaaccg cggaaaggat tcggaaccgt
      601 cgcgttcaga aaagtttggg ttgccgcaat cgtgttcaga atcgtcccgc cgtccgtcgc
      661 gttttcaaag ttgttaagat attcgcgcgg aatataattc agttgacgca gaaccggcaa
      721 attcgcgccg cgcgagtaaa cgaaagtcgc ttcgacgccg atgtcgaagg gcagttcgcg
      781 ctggatgccg aagacgaatc tggtgtagtt tgcgtttttc cgctcgtatg tgtaaagcgt
      841 cgcagcggtc ggtccggtcg cgttggtcgt tccgagcgtc gttccgacca gtgtatttaa
      901 accctggctc gaaccgattg ccggatttaa accgcccgga aacggattgt tgatgtttgc
      961 cagaaatgtt aaaccgttat tggttgacgg cgtaaaagtc gtattcgcgg taaagccggc
     1021 ttggttaatc ggctgtatct gaaacggcgc ggtgaaaatc ccgaaaccgc cgcgaataac
     1081 cgttttatcg ttaagcgcgt aggaaaaacc gaggcgcggc tggaaattgt ttttgtcaat
     1141 cgcctgattg gactggctgc cgttttcggc aaagcgcaag ccgccggaaa gattctgaaa
     1201 cgccgcgacg ggaacgctcg ccggcacgtt ggcgttgaaa ttcgtcaacg ctccggcgcg
     1261 cagcgggctc gccgtcgtgg tgtcgaatcc ggtcacaaaa cgaccttcgg cttcgcgcaa
     1321 gccggattcc agctcgtaac gcagcccgag attgagagtt aatttctggg tgacgcgcca
     1381 gtcgtcctgc acgaaaaagc cgttgtaatt catactgacg tcgtaggtcg ccgcctgctc
     1441 gataaagctg ccgctggtgt ttgccgacgg gatgcccagt aaaaatgaag ctaaatcgcg
     1501 cccgagggcg ttaacggtcg tcgcgttgga gtttgaagcc ggagcggtaa aagcgccggt
     1561 gaacagaaag cgtccggcgt tgtagccgtt cgtcgagcga ttttcccaga gcttgcggta
     1621 atcgtagccg tatttcaaag tgtgatttcc gaaaatctgc gtcagcgtcg gctgcaggga
     1681 aaacaatctg aaatttctcg tcaagccttc attaaaatcg gcgcgctccg aaccgagcgt
     1741 gtcgtagttg gtaaaatcga agcgcgggaa aacggtcgat tcgctgacgg cggcgattcc
     1801 gttaaagccg agatcggcgg cggacatcgg atttgccggg attctctgct gcacgaaatc
     1861 gttcaaactg gagcgaatat cgagcacgaa atttgaagaa agcgtcgccg tatagtcgag
     1921 gctgccgccc ttgttggtgc ggatttcaaa gccttgcgta atggaatccg gctcgccgat
     1981 aaagttgtac cggtcttcgt tgctcttgct gtagaaaaac ttgccgaaga tgcgatgctc
     2041 ggcgttgaaa ttatggtcga ttctggtcag atacgaatca tacgggcgcg aaagactctg
     2101 gttggaaaaa taattatcga aggtgcccgg ctgattcggc agcggataaa gattcagaaa
     2161 agcgaccgcc gccggattca gacgattcac cggaataata tttcccgcga aagccgtgcg
     2221 gcagacgacg ctggcggcgc agccgttgtt gcccgtgcct aaaaatgccg tcgccgggtc
     2281 gtaaatcggc gtgcccggca gcagctctga aaagtctccg tttctcatcc gcacggtcgg
     2341 aacggtaaaa gtttccggct cggcgcgctc gtcgagctgt ttttcgtaag agaacataaa
     2401 aaacgtgcgg tcctgcccgt tgtaaatttt cggaatataa accggaccgt tgacctgtcc
     2461 gccgtaacga taatattgac gcggcgggcg ctcggagcca gcccggttgg cgaaaaagtt
     2521 gttggcggtc aaagcttccg ggcgattgaa ataatacagc gagccgtgca gcttattcgt
     2581 tccgcttttg accgcgacgt tgacggtcga accagcggta aaaccgctct gcgcgtcgaa
     2641 ggcgttggtc tgaatcttaa attgcgtcag cgcgtcagcc ggcggcgtgt aggcgactcc
     2701 gccgtcaaag gtcagattcg gcgagccgtc cagcgtgatt tgattaccag ccgcgccgtt
     2761 ggtacggatg gcggagagat ttccgttcgc cgtcggtccg gtaaattgcg gatttcccgt
     2821 gtaagagacg cccggcgcct gcgtcgccag attgtaagcg gcgccttccg acaagggcag
     2881 ctcttcgatt tgcctttgcg taatgacggt tccggtcgtg acagtgcctc tttccaccaa
     2941 ttccgtgtcg gcgataatat cgacatccgc cgtctgcccg atttccaatt gaacatcaat
     3001 cgtcagacgg tcgtcgacgt tgacggtgac gttttcacga accgatgttt taaaaccggc
     3061 ggcagtaatc gaaacggaat atctgccggg cagcaaaaac gggacggtgt aggagccttc
     3121 gtcattcgcc gtgacgttat taacgacgtt ggtatcgaga tttttgacgg cgacggtcgc
     3181 gccgggaacc accgcgccct gcgggtcgct gacggtcagc cgcacggtga ccgtcgccgg
     3241 cgccgtcgcc gcgtaggtct tgaccggccg cacacccgtg gcgacggtcc cgtccccgag
     3301 gtcccagcgg taggtcagcg cgtcgccgtc gaggtcggtg gagcgactgc cgtcgaaggt
     3361 gaccgtccgc gtggccgcgt tcgtggtgaa cgtcgcctcc gccgtcggcg ggcggttgcc
     3421 gggcaggtag accagccgct tgagccggct gccggcgatg tccgcgtaga cgatgtcgcc
     3481 gttggcggcg gtgccgaact tgaccggcag cccgttctcc gcgccgaagc cggccgcctc
     3541 gggctggcgg gtcacccggc cctgggcgtc
//
LOCUS       DASWNE010000492         3552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_228525, whole genome shotgun sequence.
ACCESSION   DASWNE010000492 DASWNE010000000
VERSION     DASWNE010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228525"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="VGB68_09680"
     CDS             <1..683
                     /locus_tag="VGB68_09680"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0049543.1"
                     /translation="DRKTDIAVFRDGDWYILQSSNNTFRAQHWGAPGSDTAVPADYDG
                     DGRADLAVFRAGAEASSPTYFFILRSTSNTEQTQQFGTTGTDRAFPADYDGDGKADIA
                     VYREAGGIWYILQSSDNNLRGAQFGLGNFQDQPVPRDYDGDGKTDLGVYRKSSGTWYL
                     LQSTAGFAGAQFGISTDLPVPADFDGDGKSDLGVYRDGTWYLLRSQLGFGAFRFGLAG
                     DTPIPSVP"
     gene            828..1136
                     /locus_tag="VGB68_09685"
     CDS             828..1136
                     /locus_tag="VGB68_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049544.1"
                     /translation="MNDSILPVACTLNDAEFREREQVILRKLMQTAVERKELADGFDF
                     RFSPDDDLLREITEMIILERKCCPFLDFKIALKAGNADIWLEITGAAGAKEFIATAFK
                     "
     gene            1212..1748
                     /locus_tag="VGB68_09690"
     CDS             1212..1748
                     /locus_tag="VGB68_09690"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0049545.1"
                     /translation="MDYIIRKANLEDRAAIERLIAESARGLSRDKYSNPQIEAAIAMV
                     FGVDTDLILDGTYLVVEADGAIVGCGGWSKRKTLFGGDRFAVRDSSRLDPQTEAARIR
                     AFFVHPDYARKGIARAILSECESAAREHGFRSLELMATLPGIKFYEAHDYKGKELVEY
                     EIAEGIRMEFLPMRKEFN"
     gene            complement(1815..2438)
                     /locus_tag="VGB68_09695"
     CDS             complement(1815..2438)
                     /locus_tag="VGB68_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049546.1"
                     /translation="MKKFYIFFGTLLLIYFSLFFAPDAFGQRRGGGGGSVGGTYTGTV
                     LSYGSGFNTRTVTRPFTLSITGETSDEDAVRFLGILQNRGQSTLLSEINDEDLGYFSV
                     GAQLGRRLNVVRESVEGGQRRIFIVFERWTQFAEVRGGYRSLDYPFGVIELIIDERTG
                     RGEGTYIAAARIRWTRNGSDANQVEVENFATFPARLLSVRRSGGRGR"
     gene            2557..3090
                     /locus_tag="VGB68_09700"
     CDS             2557..3090
                     /locus_tag="VGB68_09700"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEY0049547.1"
                     /translation="MIKYAAFLRGINVGGHHIVKMEELRRIFAALGFENVKTYIQSGN
                     VIFETAETDADFLAEKIERELKQAFGFEVKTMPRTIAEVEEIAARNPFRQPETGAKIY
                     VCLLSAAPAADLKDSLAAFENDFEDFRLRNRELYWMIRPHNTRKELFSNNFIEKHLKV
                     AATTRNITTINKMLLLK"
     gene            3290..>3552
                     /locus_tag="VGB68_09705"
     CDS             3290..>3552
                     /locus_tag="VGB68_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049548.1"
                     /translation="MKNAEEYADIIIRDEREKIVSETPTVYEDDKIERIYEFADGAVV
                     KYEWQDAPRAEPFNHKFTLIKLPSPNPQRFRAGVVKVVNYPVNS"
BASE COUNT          898 a          889 c          943 g          822 t
ORIGIN      
        1 gcgaccgcaa gaccgacatc gccgtttttc gcgacggcga ctggtatatc ctgcaaagct
       61 cgaacaatac ttttcgcgcg cagcactggg gcgcgccggg cagcgacacg gcggttccgg
      121 ctgattacga cggcgacggt cgggcggatt tagccgtttt tcgcgccgga gccgaagctt
      181 cctcgccgac ctactttttt atcctgcgca gcacgtcaaa caccgagcag acgcagcaat
      241 tcggaacgac cggaaccgac cgcgcgtttc cagccgatta cgacggcgac ggcaaagccg
      301 atatcgccgt ttatcgtgaa gcgggcggca tctggtatat cctgcaaagc tcggataata
      361 atctgcgcgg agcccagttc ggactcggca attttcaaga ccagcccgtg ccgcgcgatt
      421 atgacggcga cgggaaaacc gatttgggcg tttatcgcaa gtcgagcgga acctggtatt
      481 tactgcaaag caccgcggga tttgccggcg cgcagttcgg catttcaacc gaccttccgg
      541 ttccggcaga tttcgacggc gacggcaaat ccgatttggg cgtttatcgc gacggcactt
      601 ggtatctgct gcgttcccaa ctcggtttcg gcgcattccg attcggtttg gctggcgata
      661 cgccgattcc gtcggtgccg taagagcgca aaagattgat gagaggagaa tgggacgccg
      721 gtttattagg atttgcttag aataaatcag gatttttatt tgaatttatc ctgatttatt
      781 ctaaattatc ttaaataacc ggcgttttgt ttcccggtaa aaaaattatg aacgattcga
      841 ttttgccggt cgcctgcact ttaaacgatg ccgaatttcg cgagcgtgag caggtgattt
      901 tgcggaagct tatgcaaacg gcggttgagc gaaaagaact tgccgacggt ttcgattttc
      961 gattttcgcc cgatgacgat ttgctcagag aaattaccga aatgataatt ctcgaaagaa
     1021 aatgctgtcc ttttctcgat tttaaaatcg cgctcaaagc cggaaacgcc gacatttggc
     1081 tggagataac gggcgcggcg ggcgcgaaag aattcatcgc gacagctttt aaataactga
     1141 aaagcgagag ttgcgaattt attttccgtt tatcttattc gtggctcaca gttcccgctt
     1201 ctcgcttaaa aatggattac atcatcagaa aagcgaatct cgaagaccgc gcggcaatcg
     1261 aaaggcttat cgcggagtcg gcgcgcgggt taagccggga taaatattca aacccgcaaa
     1321 tcgaagcggc aatcgcgatg gttttcggcg tcgatacgga tttgattttg gacggcacgt
     1381 atctggtcgt cgaagcggac ggcgcgattg tcggctgcgg cggctggagc aagcgaaaga
     1441 cgcttttcgg cggcgaccga tttgccgttc gcgattcgag ccgtctcgac ccgcagacgg
     1501 aagcggcgcg gattcgggcg tttttcgttc atcccgatta cgcgcgaaaa ggaatcgcgc
     1561 gggcgatttt gtccgagtgc gaaagcgcgg cgcgcgagca cggctttcgc tcgctcgaac
     1621 tgatggcgac gcttcccggc ataaagtttt atgaggcgca cgactacaag ggcaaagagc
     1681 ttgtcgaata cgaaattgcc gaaggcatca ggatggaatt tctgccgatg agaaaagagt
     1741 ttaattaatg caaaacgcgg aatgcgcaat taaatttgat gatttaattg cgcattccgc
     1801 gttttgcatt ttatttaacg tccgcgtccg ccgcttcgcc tgacgctcag aagccgcgcc
     1861 ggaaaggtcg caaaattttc gacttcgacc tgattggcat cgctgccgtt gcgcgtccag
     1921 cggattcggg cggcggcgat gtaagttccc tcgcctctgc ccgtgcgctc gtcgattatc
     1981 aattcgataa cgccgaacgg gtaatccagc gagcggtagc cgccgcgcac ctcggcaaac
     2041 tgcgtccagc gttcaaaaac gataaagatt cggcgttgcc cgccctcgac gctctcgcgc
     2101 acgacgttca ggcgacgtcc gagctgcgcg ccgacgctga agtagccgag gtcttcgtcg
     2161 ttgatttcgc tcaacagcgt gctctgaccg cgattctgca aaatgccgag aaatctgacc
     2221 gcgtcctcgt cggaagtttc cccggtaata ctaagcgtaa acgggcgcgt gacggttcgg
     2281 gtgttaaagc ccgagccgta gctcaaaacc gtgcccgtgt aagttcctcc gacgctgccg
     2341 ccgccgccgc cgcgccgttg cccgaaagcg tccggagcga aaaacagcga gaaataaatc
     2401 agaagcagcg taccgaaaaa aatataaaat tttttcatat caattgtccc ctcgttcgat
     2461 aaaatattct ttattatttt ataccacaac ggagttttat tttttgttcg attacgcaca
     2521 ttgagcgata atagtttttg gctgaggaga gcgaaaatga ttaaatacgc ggcgtttctg
     2581 cgcggcatta atgtcggcgg gcatcacatc gtgaaaatgg aagagctgcg ccggattttt
     2641 gcggcgctcg gttttgaaaa cgtgaaaacc tacatacaaa gcggaaacgt aattttcgag
     2701 acggcggaaa cggacgccga ttttctcgcc gaaaaaatcg agcgcgagct gaagcaggct
     2761 tttggtttcg aggtcaaaac aatgccgcgc acgatcgccg aagtcgaaga aatcgccgcg
     2821 cgcaatcctt tcaggcagcc ggaaaccggc gcgaaaatat acgtctgtct tttatccgcc
     2881 gcgcccgccg ccgatttgaa agattcgctc gcggctttcg aaaacgattt tgaagatttc
     2941 cggctcagaa atcgtgaatt gtattggatg attcgaccgc acaacacccg gaaagaattg
     3001 ttttccaaca attttatcga gaagcatctg aaagtcgcgg cgacgacgcg caatatcacg
     3061 acaatcaata aaatgctgct cttgaaatga aaggcttaaa aaaatgattt accgcgtccg
     3121 gaaaagacaa tcgagaaagc tttctcgcag tttgaagcga ttttcgaaaa tccgggcgtc
     3181 gaggtttacg acagagatat cgacccgaat agtgaaaaac gatgaacgat aaacgacgaa
     3241 ttgcaattgc agcgccggaa ttcatcattc atcgtctttt taaaaactta tgaaaaatgc
     3301 cgaagaatac gccgacatta ttattcgcga cgagcgagaa aaaatagtat cggaaacgcc
     3361 gaccgtttac gaagacgata aaatcgaaag aatctacgaa ttcgccgacg gcgcggtcgt
     3421 taaatacgaa tggcaggacg cgccgcgcgc cgaaccgttt aatcacaaat tcactttaat
     3481 aaaattgccc tcgcccaatc cgcaaagatt tcgggcgggc gtcgtgaagg ttgtaaacta
     3541 tccggtcaat tc
//
LOCUS       DASWNE010000493         8303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_86128, whole genome shotgun sequence.
ACCESSION   DASWNE010000493 DASWNE010000000
VERSION     DASWNE010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8303
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86128"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VGB68_09710"
     CDS             complement(<1..512)
                     /locus_tag="VGB68_09710"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0049549.1"
                     /translation="MKETTGKAELSKSAADIPAIGTGSSAFESAVTVTVDDRLGTRIG
                     KRRWLICALLFFATTINYIDRQVLGILATDETFKAEIGWNEAEYGFVNTAFQAAYAIG
                     LLVVGGLMDKFGTRRGFSVAIVFWSIAAMGHALARSAFGFGVARFTLGLGEAGNFPAA
                     IKTVAEWFPK"
     gene            complement(607..1725)
                     /locus_tag="VGB68_09715"
     CDS             complement(607..1725)
                     /locus_tag="VGB68_09715"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEY0049550.1"
                     /translation="MNSEIEIKTERLQKLLARENAGGVLINAQHNFAWLTGGKSGGID
                     LSKENAACFLLVGGDGKRFVLANNIEMPRLLTEEISEADFEPVEFSWQDEKASGNFIF
                     EKAKSLLPQNAELVSDLLLSSSARSIESLIARCRYELTEAEIERYRHLGKDAGTAIGD
                     LFKHLKPGETETEIARKVRDALAAYNINPIVALIGADARIENFRHPVPTSNVWEKVLL
                     IAICANREGLLANLTRIACIGKIPDALKRKTEATAYVFARLLAETKTGASGAKLYEIA
                     AAAYAEKGFADEINRHHQGGATGYKTRDWVAHPASAEIVSKNQAFAWNPSITGTKTEE
                     TCLVFSDRIETITQSASFPQTAIEIGGREYLSPGILSL"
     gene            complement(1896..2672)
                     /locus_tag="VGB68_09720"
     CDS             complement(1896..2672)
                     /locus_tag="VGB68_09720"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY0049551.1"
                     /translation="MSYAKFDLSGKTAVVIGGTSGIGRALAHGLAEAGADVAATSRRL
                     EQVAATAAEIEARGRRSLRIASDVSDRESLENLLNECIDEFGKVDILVNSAGKTKRQP
                     TLDVSEEDWNDILETNLSGTLRACQIFGRHMVERGYGRIINIASLSTFVSLFEVAAYS
                     ASKAAVASLTKSLAVEWASRGVNVNAIAPGVFRTPLNEKLLDESERGREFLTRTPMHR
                     FGQVEELAGAAIYLASDAASYVTGSVLVVDGGFLASGVNQ"
     gene            2905..3870
                     /locus_tag="VGB68_09725"
     CDS             2905..3870
                     /locus_tag="VGB68_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049552.1"
                     /translation="MFSKSSAKKNIKRRKVGNNVWLGYAGDGADLKDFTRRKFVFEFE
                     VLETEEAVGQAMLDELLAYSKLKTGDIVIILLGGRGAQAMYKIINKLSQTGEIDALLA
                     RLHVFTQDALAPMRMNNGLSFVRDFERLLGDDFFQKIKSFTPMNTDTDDLEGELIEYL
                     EKLERLGGIDIFFLGLGPEAAAASHLAYIKPDSGANFSDIAGVIPISESILEHHINKF
                     KVGGSRVSEADETECRSATHILTLGPAAILGAKKIVQSIVDASTAPAKRASFKRLIET
                     ELSENESRLQRQLDENPGLWIRLHPNVRSLILPDVLSEPPAAAGG"
     gene            4003..5577
                     /locus_tag="VGB68_09730"
     CDS             4003..5577
                     /locus_tag="VGB68_09730"
                     /inference="COORDINATES: protein motif:HMM:NF045169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HEY0049553.1"
                     /translation="MSNTYKQPAVPIGDYAIIVNPDDNVAVVKNETSEGLAVFLPDQE
                     TVTLSQAVPPGHRFATRDIPAGSFVYQYGQPIGTSLGIAVGEWITHANMSNEVPIVRD
                     LPENLHTPAPDYFSKDEIETFMGFRRPDGRVGTRNFILIVPTSMCASHEALQISMMSE
                     FLHYKREKYPNVDGVVAIPHNKGCGCQDGSNLDIMLRTLANYADHPNVGGVILMDLGC
                     EKTNLAKVEKYLLKRELSFDKPVAKIGIQEIGGTQAAIEKGLKIVQEMLPQVNENVRE
                     QFPVSELVLGVKCGSSDGFSGISANPSLGRAADFLVRSGGTVLITEVPEFCGAEHILA
                     NRAKDAETGRAIYRMIDWYKDYASKFGGVLNNNPSPGNIAGGLLNITIKSLGAMAKAG
                     TTRVEGVVEYAEAPKTRGLNLMQGPGYDQESTPALVAGGATIVVFTTGNGTTIGNAIA
                     PVIKLSSNNRIFEHMSRDIDISAGNVIEGTETIDEVGERLFEHIRRVASGEIPARAEE
                     SKHREFQFWAEQTVSL"
     gene            5598..6818
                     /locus_tag="VGB68_09735"
     CDS             5598..6818
                     /locus_tag="VGB68_09735"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEY0049554.1"
                     /translation="MNDSSVYQSDLLNTETGTPNIARKGNLLLRLIRAAQPISRTEIA
                     ARLSIDKSTVTENVKPLLAAGILREETLESDVSKGRKPRVLSFARDNDYFIGVNLGVR
                     TSQVGLTTLKGSILSEDDFATPPKAEAALKLAREKIENLIRENADKNLRVIGVSVPGL
                     TDTERRTLVYAPNLNWRNVNVAEAFSVNQGVAVVVENDATAAAMYEARLKIRHSNDGL
                     MTNFILVRSGTGIGVGMVIGSEVYRGTGLARGIAGEFGHMTIVAGGKSCVCGNNGCWE
                     KYASAASAASLYHGDRPLRPYETMPRFIDIVAKAENGDTRSRRTLEKIGDYLGIGIAN
                     VIMGVGIPRVIISGRLVYGWQFIEKPLKEAIARSIVGKLDGWSIEAGEPAGAALGGAL
                     EVAVEEYLTNGLSV"
     gene            6953..7786
                     /locus_tag="VGB68_09740"
     CDS             6953..7786
                     /locus_tag="VGB68_09740"
                     /inference="COORDINATES: protein motif:HMM:NF016826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HEY0049555.1"
                     /translation="MKEQIAPENIERETCVVKATHRGKGRRSAVSPGKTAARHLHYGR
                     ITLEAGDASLGFENETHETGLICLNGSAEVSTGGQIFTLNRYDSIYIPRDSKIEVSGA
                     SGCDLAEISAPVENRYPLQFVAFEDIRQNPSLHFIAGKPPIERDLNILLGKNVEAGRI
                     MADVTFSSDGNWTSFPPHEHSEMLEEAYLYIDMPAPQWGIQMVYTNPQEPELIEVVRE
                     GDVVIMPRGYHPNVAAPGGSINFLWMMAANREGVDRQFGVVNVQPEYAERGSGLEAAG
                     K"
     gene            7909..>8303
                     /locus_tag="VGB68_09745"
     CDS             7909..>8303
                     /locus_tag="VGB68_09745"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0049556.1"
                     /translation="MILDKFRLDGKAALVTGASVGLGQAMAIALAEAGADVAAHCHMA
                     GEADETCQKISETGRKTFSVAGDMAERDVPQSLVEKVIAEFGRIDILINNAGMIRRAP
                     AVDFSEEDWTSVLEVNLSGVFRLSQAAGR"
BASE COUNT         1995 a         2118 c         2230 g         1960 t
ORIGIN      
        1 tttttcggaa accattcggc gaccgtcttt atcgcggcgg gaaaatttcc ggcttcgccc
       61 aagccgagcg taaatcgcgc gacgccgaaa ccgaaagccg agcgcgccag agcgtgtccc
      121 atcgccgcga tgctccagaa aacgatggcg acgctgaagc ctctgcgcgt cccgaatttg
      181 tccatcaagc cgccgacgac cagcaagccg atggcatacg cggcttgaaa cgcggtattg
      241 acaaagccgt attcggcttc gttccagccg atttccgctt taaaagtctc gtcggtggcg
      301 agaattccca aaacctgccg gtcgatgtaa ttaatcgtcg tggcgaaaaa caagagggcg
      361 caaatcagcc agcggcgctt gccgatacgc gtgccgaggc ggtcgtcgac ggtcacggtc
      421 accgccgatt caaacgcgct cgaacccgtg ccgattgccg gaatatccgc cgccgatttt
      481 gataattccg cttttccggt tgtttccttc attgtctatt acctcaaatg cagtgatttt
      541 taccctaacc cgtccggaaa aatactaagt ttttatgttt ccggacggct tcgcctgatt
      601 tcctgtttat aaactcaaaa taccgggaga aaggtattcg cgcccgccga tttcaatcgc
      661 tgtctgcgga aagcttgccg attgcgtaat cgtctcgatc cggtcggaaa aaaccaggca
      721 ggtttcttcc gtttttgttc ccgtaatgct cggattccag gcgaaagcct gatttttaga
      781 gacgatttcg gcgcttgccg gatgcgcgac ccaatcgcgc gttttgtatc cggtcgcgcc
      841 gccctgatga tgccggttta tctcgtccgc aaaacctttt tcggcataag cggcggcggc
      901 gatttcataa agcttcgcgc cgctcgcgcc ggttttcgtt tccgccagaa gcctggcgaa
      961 aacgtaagcg gtcgcttcgg tttttcgctt caacgcgtcg ggaatttttc cgatacaggc
     1021 gatgcgcgtc aggttggcga gcaagccttc gcggttggcg cagattgcaa tcaaaagcac
     1081 tttttcccag acatttgacg tcggaaccgg atgccggaaa ttttctatcc tcgcgtccgc
     1141 gccgattaaa gcgacgattg gattgatgtt atacgcggca agcgcgtcgc ggacttttcg
     1201 cgcgatttcc gtttcggtct cgccgggctt taaatgttta aataaatctc cgattgccgt
     1261 gcccgcgtct tttcccagat gtcggtaacg ctcgatttcc gcttccgtca gctcgtaccg
     1321 acaacgcgcg attaagcttt cgattgaacg cgccgaagag cttaaaagca aatcggaaac
     1381 caattcggca ttttgcggca aaagagattt cgctttttca aagataaaat tcccggacgc
     1441 tttttcgtcc tgccacgaaa actcgaccgg ctcgaaatcc gcttcggaaa tctcttccgt
     1501 taaaaggcgc ggcatctcga tattgtttgc cagaacaaat cttttcccgt cgccgccgac
     1561 cagcagaaaa cacgctgcgt tttctttgct caaatcaatg ccgccggatt ttccgcccgt
     1621 cagccacgcg aaattgtgct gcgcgtttat caaaacgccg cccgcgtttt cccgagccag
     1681 cagtttttgc agtctttcgg ttttgatttc aatttcgctg ttcacaaaat aatgtcttaa
     1741 tgcaggcaga gaaggaattg accgggagtg aaggcgataa gcaggcaata agaaaaagat
     1801 ttaaaatttt tctttaatct tctttattgc tttcaatccc gggttaattt tctctgcatt
     1861 atttgtgctc tcgttttctt gtccaaaaaa ctcttttatt gatttacgcc cgaagccaga
     1921 aatccgccgt cgacgaccag caccgagccg gtcacgtaac ttgccgcgtc cgacgccaga
     1981 taaatcgccg cgcccgccaa ttcttcgacc tgaccgaagc gatgcatcgg cgtccgcgtc
     2041 aaaaattcgc gcccgcgctc gctttcgtcc agtagctttt catttaaagg cgtgcggaaa
     2101 acgccgggcg cgatggcgtt gacgttgacg ccgcgcgacg cccattcgac ggcgagcgat
     2161 ttcgtcaaag aagcgaccgc cgctttgctc gccgaataag cggcgacttc gaaaagcgat
     2221 acgaaagtgg aaagcgatgc gatgtttata atgcgcccgt aaccgcgctc gaccatatga
     2281 cgcccgaaaa tctggcaggc gcgcaaagtt cccgacaggt tggtttcgag aatgtcgttc
     2341 cagtcttctt cgctgacgtc gagcgtcggc tggcgcttgg tttttccggc ggaattgacc
     2401 agaatatcga ctttgccgaa ttcatctata cattcattca aaagattttc cagcgattcg
     2461 cggtcggaaa cgtcggaggc gattcgcagc gaccgccgcc cgcgcgcttc gatttcggcg
     2521 gcggtcgctg cgacctgttc cagccgccgc gaagtcgcgg caacgtctgc gcccgcttcc
     2581 gccagcccgt gcgcgagcgc gcgcccgatg ccggatgttc cgccgataac gaccgcagtc
     2641 ttgccgctca aatcaaattt ggcatagctc ataaattaaa tttcgggact cttttaacga
     2701 aaaattaagc aaaggtaata ccattcggta aatgttttag tagtgtgtaa caccattata
     2761 tcaattgtca agtccgaatt ctttactttt tactcgtatt ttagtagatt attaaatgcc
     2821 gtcgaattgt ttcgccgaaa aatcgtaaaa tcagaattgc attaaattgg ttaaaccaat
     2881 taaaatattt ccaagttaaa agagatgttt agcaaaagtt ctgccaaaaa aaatataaag
     2941 cgccggaaag tcggaaataa cgtctggctc ggatatgccg gcgacggcgc ggatttaaag
     3001 gatttcacaa ggcgcaaatt cgtatttgaa ttcgaagttc tggaaaccga ggaagccgtc
     3061 gggcaggcga tgctcgacga gcttctcgct tattcgaaac taaagaccgg cgatatcgtg
     3121 ataatcctgc tcggaggacg cggcgcgcag gcgatgtata aaatcattaa taaattatcg
     3181 cagaccggcg agattgacgc gctgctcgct cgactgcacg tttttacgca ggacgctctc
     3241 gcgccgatgc ggatgaacaa cggtctgagc ttcgtccgcg atttcgagcg attgctgggc
     3301 gacgattttt ttcaaaaaat caaaagcttt acgccgatga acacggacac cgatgatttg
     3361 gaaggcgagc tgatcgaata tctggaaaag ctggagcgtc tgggcggcat cgatattttc
     3421 tttctcggct tgggaccgga agccgccgcc gcttcgcatc tcgcctatat caaaccggat
     3481 tcaggcgcga attttagcga catcgcgggc gttatcccga tttccgagag cattctggaa
     3541 catcacatca acaaattcaa agtcggcgga agccgcgtct ccgaagccga cgaaaccgag
     3601 tgccgcagcg ccacgcatat tttaacttta ggaccggcgg cgattttggg cgcaaaaaaa
     3661 atcgtgcagt caatcgtcga tgcttcgacc gcgccggcaa aacgcgcgag tttcaaaaga
     3721 ctaatcgaaa ccgaattatc cgaaaacgaa agccggcttc agcgtcagct cgatgaaaat
     3781 ccgggcttgt ggatacgtct tcacccgaac gtcaggtctt tgattttacc cgatgtattg
     3841 tcggaaccgc ctgcggcagc gggcggctga acgctcgatt ttcagatttc cggtgaaaag
     3901 tttcaagtta atctgaatta atctcttaac ctgaaaccta cagtatttaa aagctttaac
     3961 ttaaatcgtc ggctgacgca gtcggctctg gctattttta atatgtcgaa tacttataag
     4021 caaccagccg ttccaatcgg cgattacgcg attatcgtca accccgacga taacgtcgcc
     4081 gtcgtcaaaa acgaaacttc ggaagggctg gctgtgtttt tacctgatca ggaaacagta
     4141 actctcagcc aagccgttcc gcccggacat cgttttgcga cgcgcgatat tccggctggc
     4201 agcttcgtct accaatacgg tcagccgatt ggaaccagcc tgggcattgc cgttggcgaa
     4261 tggattacgc acgcaaatat gtcgaacgaa gtgccgatcg tccgcgattt gccggaaaat
     4321 cttcacactc cggcgccgga ttatttttcg aaagacgaaa tcgaaacttt tatgggtttt
     4381 cgccgtcccg acggcagagt cggcacgcga aattttattt tgattgtgcc gacctcgatg
     4441 tgcgccagcc acgaagccct gcagatttcg atgatgtcgg aatttctgca ctataagcgc
     4501 gaaaaatatc cgaacgtcga cggcgtcgtg gcgattccgc acaataaggg ctgcggctgt
     4561 caggacggct cgaatctcga cataatgctg cgcaccctcg ccaattacgc cgaccacccg
     4621 aacgtcggcg gcgtgatttt gatggatttg ggctgcgaga aaacaaatct cgccaaggtc
     4681 gaaaaatatc ttttaaaacg cgagctttct ttcgataagc cggtcgctaa aatcgggatt
     4741 caggaaatcg gcgggacgca ggcggcaatc gaaaaaggct tgaaaatcgt gcaggaaatg
     4801 ctgccgcagg tgaatgaaaa cgtgcgcgag caatttccgg tcagcgagct tgtgctgggc
     4861 gtcaaatgcg gctcaagcga cggtttttcg ggaatttcgg caaatccgag cctgggacgc
     4921 gcggctgatt ttctggttcg ctcgggcggg acggttttga ttacggaagt gccggaattt
     4981 tgcggagccg agcatatcct ggcaaaccgc gcgaaagatg ccgagacggg gcgcgccatt
     5041 taccggatga ttgactggta taaggattac gcctcgaaat tcggcggcgt tttaaataac
     5101 aatccgagtc cgggcaatat cgcgggcggg cttttgaaca tcacgattaa atctctgggc
     5161 gcgatggcaa aggcgggaac gacgcgcgtc gaaggcgtcg tcgaatacgc ggaagcgccg
     5221 aaaactcgcg gtttgaattt gatgcagggt cccggctacg accaggaatc gacgcccgcg
     5281 ctggtcgccg gcggcgcgac aatcgtcgtt ttcacgaccg gcaacggaac gaccatcgga
     5341 aacgcgattg cgcccgtcat taaattatct tcgaacaatc gcatttttga gcatatgagc
     5401 cgcgatattg atatttcggc gggaaacgtg attgaaggca cggaaacgat tgacgaagtc
     5461 ggcgagcggc ttttcgagca tatccggcgc gtggcgagcg gcgaaattcc ggcgagagcc
     5521 gaagaaagta aacatcgcga gtttcagttc tgggcagaac agacagtttc attgtagaat
     5581 ttgatttttt aaatactatg aatgattcat ccgtctatca atccgatttg ctaaatactg
     5641 aaacaggaac accaaacatc gcccgcaagg gaaatttact tttgcggctg attcgcgccg
     5701 cgcagccgat ttcgcgcacc gagattgcgg cgcgtctgtc gattgacaaa agcaccgtca
     5761 cggaaaacgt caagcctttg ctggcggcgg gaattctgcg cgaagaaacg ctcgaatccg
     5821 acgtctccaa aggacgcaag ccgcgcgttt tatcgtttgc ccgcgataat gattatttta
     5881 tcggcgtcaa tctgggcgtg cggacttcgc aggtcggttt aacgacgctg aaaggctcga
     5941 ttttatccga agacgatttt gcgacgccgc ccaaagcgga agccgcgctg aagctggcgc
     6001 gcgagaaaat tgaaaattta attcgtgaaa acgccgacaa aaatctccgt gttatcggcg
     6061 tcagcgtgcc gggcttgacc gatacggaac gacgcacgct cgtctacgcg ccgaatctga
     6121 actggagaaa cgttaacgtc gccgaagcgt tcagcgtcaa tcagggcgtt gcggtcgtgg
     6181 ttgaaaacga cgcgaccgcc gccgcgatgt atgaagcgag attgaaaatt cgccattcaa
     6241 acgacggctt gatgacgaat tttattctgg ttcgttcggg aacgggcatc ggcgtcggta
     6301 tggtcatcgg cagcgaagtt tatcgcggaa cgggactggc gcgcggaatc gccggagaat
     6361 tcggtcatat gacgattgtg gcgggcggca aatcctgcgt ttgcggcaat aacggctgtt
     6421 gggaaaaata cgcttccgcc gcttccgccg cttcgcttta tcacggcgac cgacctttgc
     6481 gtccatacga gacgatgccg cgatttatcg acatcgtcgc caaagccgaa aacggcgaca
     6541 cgcgttcgag aagaactttg gaaaaaatcg gcgattatct cggcatcggc atcgccaacg
     6601 tgattatggg cgtcggcatt ccgcgcgtaa ttatcagcgg acgcctggtt tacggctggc
     6661 agtttatcga aaagccgctc aaggaagcga ttgccagaag catcgtcggc aagctcgacg
     6721 gctggtcgat cgaagcgggc gagccggcgg gcgcggcgct cggaggagct ttggaagtgg
     6781 cggtcgagga gtatttaacg aacggcttga gcgtttgaaa gcagaaaagt gaaagcgtga
     6841 aaagcgtgaa aagcgtgaaa aagctcgtgt cgctttccgg cagccgaact ttttcatctt
     6901 tttacgtttt cacgcttttg cacgaattat caatcagagg gtttaaggca ttatgaaaga
     6961 acagatagct ccggaaaata tcgaaaggga aacctgtgtc gtcaaagcga cgcaccgggg
     7021 caaaggcaga cgcagcgccg tttcgccggg aaaaacggcg gcgcggcatc ttcattacgg
     7081 gcgcatcacg ctggaagcgg gcgacgcttc gctcggtttt gaaaacgaaa ctcacgaaac
     7141 gggtttgatt tgtttaaacg gctcggcgga agtctcgacc ggcgggcaga tttttacatt
     7201 gaatcgttac gattcgattt atattccgcg cgactcgaaa attgaagtct ccggcgcgag
     7261 cggctgtgat ttggcggaaa tctccgcgcc ggtcgaaaac cgttacccgc ttcaattcgt
     7321 cgcctttgaa gacatacggc aaaatccttc gctccacttt atcgccggaa aaccgccgat
     7381 tgagcgcgat ttgaatattc ttctgggaaa aaacgtcgaa gccggacgca ttatggcgga
     7441 cgtcactttt tcgtcggacg gcaactggac ttcctttccg ccgcacgaac attccgagat
     7501 gctcgaagag gcgtatctgt atatcgatat gcccgcgccg cagtggggaa tccagatggt
     7561 ttacaccaat ccgcaggagc cggagctgat tgaagttgtc cgcgaaggcg acgtggtaat
     7621 aatgccgcgc ggttatcatc cgaacgtcgc cgcgccgggc ggctcgataa attttctgtg
     7681 gatgatggcg gcaaatcgcg aaggcgtgga cagacaattc ggcgtcgtta acgtccagcc
     7741 cgaatatgcc gaacgcggtt cggggctgga ggctgccgga aaataattca aacggcgatg
     7801 aaggcgatag agaagattga taaagtaaaa agtaaaatca caattttatc ttttaacttt
     7861 tcttatcttt ttcatctcct ttatctctgt taatttcttt tttaaaaaat gattctcgat
     7921 aagttcaggt tagacggcaa ggctgcgctc gttacgggcg cgtcggtcgg gctcgggcag
     7981 gcgatggcga ttgctctggc ggaagcgggc gcggatgtcg cagcgcattg tcatatggcg
     8041 ggcgaggctg acgaaacgtg tcaaaaaatt tccgagaccg ggcgaaaaac tttttcggtc
     8101 gccggagata tggcggaaag ggacgttccg caaagtctgg tcgaaaaagt tatcgccgag
     8161 tttggtcgga tagatatttt aatcaacaat gcgggaatga ttcgccgcgc gcccgccgtc
     8221 gatttttccg aagaagattg gacgagcgtt ctggaagtca atctttccgg cgtttttcgt
     8281 ctgtcgcagg ctgccgggcg aaa
//
LOCUS       DASWNE010000494         2993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_612972, whole genome shotgun sequence.
ACCESSION   DASWNE010000494 DASWNE010000000
VERSION     DASWNE010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612972"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(383..1297)
                     /gene="speB"
                     /locus_tag="VGB68_09750"
     CDS             complement(383..1297)
                     /gene="speB"
                     /locus_tag="VGB68_09750"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEY0049557.1"
                     /translation="MSESATLPINFGGIAEEEFSGFEAARILVLPVAYEGTVSYGTGT
                     EAGAMAIVEASRNMELYDEETDAEVYKLGIHTLDEFAPRETPEAMMNALYERAKELLK
                     EEKFLCMLGGEHSVSAPVIRAHAEKFHNLSILQIDAHADLRDEYDGTPHSHASIMARV
                     VKDLRIPSVQVGIRSISADEARSLDSGLPTRIFWAKDIAGRIDWIDDAVNSLTENVYL
                     TIDIDGLDPSLVPTTGTPEPGGLGWYETLTLIRKLAAKKRIIGMDLVEFSKTDNSDAP
                     AFLCAKLVYKSLAYIFQNDTPKVRGKST"
     gene            complement(1412..2473)
                     /locus_tag="VGB68_09755"
     CDS             complement(1412..2473)
                     /locus_tag="VGB68_09755"
                     /inference="COORDINATES: protein motif:HMM:NF014027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HEY0049558.1"
                     /translation="MVTQRKPKASKFLTVPTRPIPVDRDRSVAGLLEKMEGAGFGAKQ
                     LAEAHRIWLDMLDDNSTIYLCCSGNLIPAGMRRLLAYVIKNRFVDVLVLSGTVIFHDI
                     HETLGRNHYQAHPSMSDEELESSEIYRIGDVLVNQDEFREADEWIGSVINQFELSRPY
                     SVREFLHLLGRELAEIAHEDGILTAAYKSRVPVFCPDLVGSELSVGIARARFEKKIQL
                     IFDAIQDTMEMMQIAQRTRNSGIITLGSTCSQNMVNVSELSAYITRTNVRGHKYAVSV
                     TTDAAPLEVRTPSIGGNHTQVFGKLLKNATTAYVASDPSIAVPMLVTALSQTAAKYMK
                     GRKRPSFTFAGKDLGIDIP"
     gene            complement(2499..2741)
                     /locus_tag="VGB68_09760"
     CDS             complement(2499..2741)
                     /locus_tag="VGB68_09760"
                     /inference="COORDINATES: protein motif:HMM:NF040441.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PF20097 family protein"
                     /protein_id="HEY0049559.1"
                     /translation="MTVEVNCPKCKSSMHEGFVIDRGDYQIKMESVWVEGKPEASFWS
                     GLKTKDRAMFTVRAYRCPNCDYLEFYTAEKTSNDWI"
     gene            complement(2769..>2993)
                     /locus_tag="VGB68_09765"
     CDS             complement(2769..>2993)
                     /locus_tag="VGB68_09765"
                     /inference="COORDINATES: protein motif:HMM:NF037681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049560.1"
                     /translation="GFIIKKVIQGATLGDSLLSARFEAGQMHDGFRRIVTQRIKDGEI
                     SMKEGNELMEFYESKINSYTYLLPNEDENS"
BASE COUNT          715 a          763 c          756 g          759 t
ORIGIN      
        1 aaaatattga aagcgtgaaa tacttttgaa tttactgaat tggttacgag aaacaaaccg
       61 tttttgttca ggacaatttg aagaaaaagc tccgaattcg caaatcttta taaacttttt
      121 ataaacttcg gcgtccggaa aaatagaatg gctcgcaaaa cactctgaac gactcgaaaa
      181 gccttctgaa tgtcatttcg agtccgttga atgacttaaa aggtctattg aacaaccccg
      241 agatgtcgtt gaacgagtcg aattgtttat tgaacgacct gaacaaggct ttgaatgcct
      301 tctgagttgc attgaatgac ttaaaaattc gattgtttga ccggaaccgt ctcgattccg
      361 cttttttcgc cctttttcgc ggttatgtgg attttccgcg aactttcggc gtgtcgtttt
      421 gaaagatata cgccagcgat ttatagacga gcttggcgca gagaaaagcg ggcgcgtcgg
      481 aattgtcggt tttagaaaat tcgaccaaat ccataccgat aatgcgtttt ttggcggcga
      541 gcttacgtat cagggtcagg gtttcatacc agccgagacc gcccggttcg ggcgttccgg
      601 tcgtcggcac gaggctcggg tcgagaccgt cgatgtcaat cgtcagataa acattttcgg
      661 tcagcgaatt taccgcatcg tcaatccagt cgatgcgtcc ggctatatct ttcgcccaga
      721 aaattctggt cggcaagccc gaatccagcg agcgcgcctc gtcggcggaa atcgagcgaa
      781 tcccgacctg tacggaggga atgcgcaaat ccttgacgac gcgagccata atcgaggcgt
      841 gcgagtgcgg cgtgccgtcg tattcgtcgc gcaaatcggc gtgcgcgtca atctgtagaa
      901 tcgagagatt atgaaatttt tcggcgtgtg cgcggatgac cggcgcgctg accgaatgtt
      961 cgccgccgag catacacagg aatttttctt ctttcagcag ttctttcgcg cgctcgtaaa
     1021 gcgcgttcat catcgcttcg ggcgtttcgc gcggcgcaaa ttcatccagc gtgtgaatgc
     1081 cgagcttgta aacttcggcg tcggtttcct cgtcgtaaag ctccatatta cgcgaagcct
     1141 cgacaatcgc catcgcgccc gcttccgtgc ccgtgccgta agagaccgtg ccttcgtagg
     1201 cgaccggcag aacgagaatc cgcgccgcct cgaagccgga aaattcttcc tcggcaatgc
     1261 cgccgaaatt tattggtaat gttgctgact cagacataat cgggctttgg tcttcggtct
     1321 ttgtttgaat gattgcgatt tcgattaaat ttaagatttc ggtaaaaaag aaaaatcgaa
     1381 gaccgaaaac caaagaccgg agaccgcatt tctaaggaat atcaattccc aaatccttac
     1441 cggcgaaggt aaagctcggg cgcttgcgac ctttcatata tttcgccgcc gtttgcgaga
     1501 gcgcggtgac cagcatcggg acggcgattg acgggtcgct ggcgacgtaa gcggtcgtcg
     1561 cgtttttcag aagcttgccg aaaacctgcg tgtgatttcc gccgatggaa ggcgtgcgaa
     1621 cctccagcgg cgcagcgtcg gtcgtcacgg aaacggcgta tttgtgaccg cggacgttgg
     1681 tccgcgtgat gtatgcggaa agctccgaaa cgttgaccat attctggctg caagtgctgc
     1741 ccaaagtaat gatgccggaa ttccgcgtgc gctgcgcaat ctgcatcatt tccatcgtgt
     1801 cctgaatcgc atcgaaaata agctggattt ttttctcgaa gcgggcgcgg gcgatgccga
     1861 cgctcaattc cgagccgacc aaatccgggc agaaaaccgg cacgcgcgat ttgtaggcgg
     1921 cggttaaaat accgtcttcg tgcgcgattt ccgccagctc gcgtccgagc aggtgcagaa
     1981 actcgcggac tgaatacggg cgcgacaatt caaattggtt gatgacgctg ccaatccatt
     2041 cgtcggcttc gcgaaattcg tcctgattga ccagcacgtc gccgattcgg taaatttccg
     2101 aagattccag ctcctcgtcg gacatcgaag gatgcgcctg ataatgattg cgccccagcg
     2161 tctcgtggat gtcgtgaaaa atgaccgttc ccgataagac cagcacatcg acaaaacggt
     2221 ttttgataac gtaagccagc agccggcgca tgcccgccgg aatcaaattt cctgaacagc
     2281 acagataaat cgtcgagttg tcgtcgagca tatcgagcca gatgcggtgc gcttcggcaa
     2341 gctgtttcgc gccgaaccct gcgccttcca ttttttccag cagtccggcg accgagcggt
     2401 cgcggtcaac cggaatcggt cgggtcggga cggttaagaa ttttgaagcc ttcggtttac
     2461 gttgggttac catagattct ccgataataa aattttctct aaatccagtc gttagacgtt
     2521 ttttcagccg tataaaactc cagataatca caattcggac agcgataagc gcggacggta
     2581 aacatcgctc tgtcctttgt tttcagtccg ctccagaatg atgcttccgg ctttccttcg
     2641 acccagaccg attccatttt tatctggtaa tcgccgcggt caatcacaaa accttcgtgc
     2701 atcgagcttt tacatttcgg acaatttacc tcaacagtca taaaagctaa aggtttccat
     2761 ttaaaaagct aggaattttc atcttcattc ggcaaaagat aggtgtagga attgattttg
     2821 ctttcgtaaa actccatcaa ctcattgcct tccttcatcg aaatctcgcc gtctttaatc
     2881 ctttgcgtga cgatgcggcg aaagccgtcg tgcatctgcc cggcttcgaa gcgtgccgac
     2941 agcagcgaat cgcccagagt cgcgccctgt ataacttttt taatgatgaa tcc
//
LOCUS       DASWNE010000495         2002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_547591, whole genome shotgun sequence.
ACCESSION   DASWNE010000495 DASWNE010000000
VERSION     DASWNE010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547591"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            424..1011
                     /locus_tag="VGB68_09770"
     CDS             424..1011
                     /locus_tag="VGB68_09770"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmG family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HEY0049561.1"
                     /translation="MENHRRSFDFELNDGQIRRLADYYELVQAHNELLHLVAPATAEE
                     FAVRHILESLTLLEFLPPRAKLADVGAGAGLPSLPCLLAREDLYGFLIESKIKKAKFL
                     DEALTKFNLFKRARILNRQFEEIEKPGGAFVTCRALDKFIPKLPKILKWSKGSSLLLF
                     GGNSLRDELKKNNVEFRAKLMPLSEQRFLFYTDKI"
     gene            1027..1563
                     /locus_tag="VGB68_09775"
     CDS             1027..1563
                     /locus_tag="VGB68_09775"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEY0049562.1"
                     /translation="MLDKLFDVDWNSIFIPGDSLLEIIIRGTLIYLICFVFLRLARRG
                     VGEIGITDVLIVVMIADAAQNGMSADYKSVTEGFVLIGTLVFWDYLLDYLSTKSKFFE
                     RVLSPPKLCLIKNGEMLRRNMRKEMITKEELMSQLRQQGIEDISEVKESYLEPGGNVS
                     IIKKKPDDGVEKREKHPR"
     gene            1659..>2002
                     /locus_tag="VGB68_09780"
     CDS             1659..>2002
                     /locus_tag="VGB68_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049563.1"
                     /translation="MKQAKKYPAPQISEAVFRGKSSLYNFEVYPMNVRFNEVPAVYII
                     SKRKIDRHGRGHHFLVCIGQTESLVEDIKKHKRGKCVKQLQANTVSVILEDDEKKRLE
                     VENDIKAAHIIP"
BASE COUNT          547 a          455 c          465 g          535 t
ORIGIN      
        1 tgccgccttc cgtgatgtcg taatcaatcc aaatccggtc gagcttgccg gtcgcttttt
       61 tcggctgcgc gttaagcgta gtattaagcg agattactac cagcagaccg agtaatcccg
      121 aaaccatcag gcgattaagc cagccgagat ttttccaaat aattttcgat gtgttcattt
      181 cctttttttt ctccgttgtg tatcttggaa taaataattg ctccacgtct aaatcgcaga
      241 atttgtggcg aaaagcggtc aaagaatttt ccgcgctcat tatgtctcat atttattcaa
      301 aataaaagcg attcaatgag tcattgacgc tttaccgatt tttttttgca ctcggaaaat
      361 tttctttttc ttcttttggc ggtatggttt aatttttcta tgcgcgacca atttataaaa
      421 gcaatggaaa accaccggag aagtttcgat tttgagctga acgacgggca aatacggcgg
      481 cttgccgatt attatgagct cgtccaggcg cacaacgaac tgcttcatct ggtcgcgccc
      541 gcgacggctg aagaatttgc cgtgcgccat attctggaat ctttgacgct tctagagttt
      601 ctgccgccgc gcgcaaagct cgcggacgtc ggcgcgggcg cgggtttgcc gtcgctgccg
      661 tgcctgctgg cgcgcgaaga tttatacggc tttctgattg aatcgaaaat aaaaaaagcg
      721 aagtttctgg acgaagctct gacgaaattt aaccttttca aacgcgcgcg aattctcaat
      781 cgccagtttg aagaaatcga aaaaccgggc ggcgctttcg ttacctgccg cgcgctggac
      841 aaatttatac cgaagctgcc gaaaattttg aaatggtcga aaggcagctc gctgctgttg
      901 ttcggcggca attcgctgcg cgacgagctc aaaaaaaaca acgtcgaatt tcgagcgaaa
      961 ctgatgccgc tttccgagca aagatttctt ttttataccg ataaaatata gtccataatt
     1021 atatttatgc tggataagct tttcgacgtc gattggaatt caatctttat tccgggcgat
     1081 tcgcttctgg aaatcatcat tcgcggaacg ctcatttatc ttatctgctt cgtttttctg
     1141 cggctggcgc ggcgcggcgt cggcgaaatc ggaatcaccg acgttttaat cgtcgtgatg
     1201 attgccgatg ccgcgcaaaa cgggatgagc gccgattata aatccgtcac ggaaggtttt
     1261 gttttaatcg gcacgctcgt tttctgggat tatctgctcg actatctcag cacgaaatcg
     1321 aaattttttg aacgagtcct cagcccgccg aagctttgtc ttatcaaaaa cggcgaaatg
     1381 ctgcgccgca atatgcggaa ggaaatgatt acgaaagaag aattaatgag ccagcttcgg
     1441 cagcagggca tcgaagacat ttcggaagtc aaggaaagct atctcgagcc cggcggaaac
     1501 gtcagcatca tcaaaaaaaa gccggatgat ggcgtcgaaa aacgagagaa acatcctcgc
     1561 tgaaaacctc taagccttta tatctaaacc gaatttccga ttatgaaatt aatggcggcg
     1621 agttgattta gcgtttcatc tatgcgacaa tattgcatat gaaacaggct aaaaagtatc
     1681 cggcgccaca aatcagcgaa gccgtctttc gcggaaaaag ctcactgtat aatttcgaag
     1741 tttacccgat gaacgtccgg tttaacgaag ttccggcggt ttatattatc tcgaaacgca
     1801 aaatcgaccg gcacggcaga ggacatcatt ttttagtctg tatcgggcaa accgaatctt
     1861 tagttgaaga tataaaaaag cacaaacgcg gcaaatgcgt caaacaattg caggcgaata
     1921 cggtttccgt aatcctggaa gacgacgaaa aaaagcgcct ggaagtcgag aacgatataa
     1981 aagccgctca tataattccc tg
//
LOCUS       DASWNE010000496        11515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_16898, whole genome shotgun sequence.
ACCESSION   DASWNE010000496 DASWNE010000000
VERSION     DASWNE010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11515
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16898"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1238)
                     /locus_tag="VGB68_09785"
     CDS             complement(<1..1238)
                     /locus_tag="VGB68_09785"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY0049564.1"
                     /translation="MATDNEPQTTDKLIWILLTIAVIFIYFFGLSVPLLGPDEARYAQ
                     VAREMFERGDWIMPTLGGFQWLEKPPLLYWLAIAAYKIFSVTEFAARFGSALFGLGTI
                     FCLWILAKFSIAANQEQNRENTKTEVRSPKIDLANWLALIAASSIGLLVFSRAASLDI
                     ILTFPMTASLVSFFIWENSRPEHEEIQRERRRSKIFAGSAALISFYFFIGVALLAKGL
                     VGIVIPFAVVAFYYLLSFKSPGKTFVFSLLWGTILSLLVACAWYLPMYRAQGWTFIDE
                     FFVQHHFQRYTSNKYQHSQWFWFFWLVLPLMTIPWLPFFAASIGSLLKGFFQRGENAE
                     TRGENGKLCAQPSALNTFALAWILAPLVFFSFSGSKLPGYILPALPGALILTADYVVR
                     FAEKSKGREIALQIKAFLMFA"
     gene            1345..2316
                     /locus_tag="VGB68_09790"
     CDS             1345..2316
                     /locus_tag="VGB68_09790"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY0049565.1"
                     /translation="MTKLRTAVIGVGSLGRHHARNYAAFAKEGAAEFVGVCDAGEETA
                     KQVAAENGCDFFTDWRALLEQVEAVSIVTPTETHSEIACAFLEKGVHVLVEKPIARTL
                     SEADSMIKAAEKSGAKLMVGHLERYNPAMVALRPHVAGPLYFEIHRVSPFPNRSLDVD
                     VVLDVMIHDLDAIQWLVGENVRVAAIHAVGIPVISDKVDAANARIEFENGAVANITAS
                     RIGTEKIRKTRFYQTNSYVVLDYATKFASVTSLAPNAIHPLLGVSINRLEIKDVEPLR
                     AEIGAFLDSIENDETPPVTAQDGRRALSLALGVLEKIDAHARKVFAV"
     gene            2313..3455
                     /locus_tag="VGB68_09795"
     CDS             2313..3455
                     /locus_tag="VGB68_09795"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY0049566.1"
                     /translation="MKSVEISAFLQSRIEAKDFPSAVYLAAEKGEIVLRDAVGLAVVE
                     PEKIRARLDTIYDLASLTKVLITGLLCAKLIETGEINLSDKAAKYFAEFDTDEKRGIT
                     LGNLLTHTSGFPAWFPFYTSFCDKNRKKECVLSVIVQSSLGNPINSKVVYSDFNFLLL
                     GFLLEKFYGESLDKLAEREIFAPLGLQNTFYNPPKELRERIAASEKGNEYEKQTCVNS
                     GFDVSNYNWRDYQIRGEVHDGNCYFMDGVSGHAGLFSTAEEVFKIARQFLPESTTLLQ
                     PETCALFRTNFTEGLNEARSVAFQLAETPDSTAGEALSRNSFGHLGFTGTSLWIDPQK
                     ERIFILLTNRTHNRRLPFVLLNSVRRRFHESAARQLNEAETGSLQY"
     gene            3612..4862
                     /locus_tag="VGB68_09800"
     CDS             3612..4862
                     /locus_tag="VGB68_09800"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0049567.1"
                     /translation="MAQTTIPSRPRINPSPSPTPADSQTQNPSPNPDRNRPPVLIGGE
                     RQQTNPVPTSTPKPGSTPTGGEDVIEEDDVIRVETNLVTIPVAVLDRDGRFVSGLRQQ
                     DFQIFEDGVPQKIENFASVEQPFTVVLLIDVSPSTQYKITEIQDAAIAFVNQLRQNDR
                     VVVISFDERVHVLSPVTNNRAILRNAILQADFGNGTSLYEAVGDALNRQLRQIEGRKA
                     IVLFTDGVDTTSSRASYESTIRDAEESEALIYPIRYDTYSDMGGGQSSGGGYGYPRRR
                     RGNRRLGDILGDILSGGTYGGGYPQGGGSAGGSREDYVRGTRYLEELARNSGGRIFQA
                     TTAANLDAAFSGIAEELRRQYTIGYYPETAGQAGQRKQVKVRVSRPNAVVRAKNSYIV
                     GGVEAQNNRTPTRQPPRIINRLPF"
     gene            5092..6291
                     /locus_tag="VGB68_09805"
     CDS             5092..6291
                     /locus_tag="VGB68_09805"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0049568.1"
                     /translation="MKKFAVVVLSIFFLFSSAGILAQSRRVTTTDGNGKANKREPKPV
                     ETPAPQATPNEETAPTVTAPAENAVEDGDVISVNTNLVTIPVRISDRGNRFIAGLKKE
                     DFKVFENDVEQEIAYFSNEEQPFTVALVLDMSLSSSFKINEIQNAAIAFTAQLRPKDK
                     VLVVSFSEQIYVLCEATSDRQRIQSAIRSSRIESGTSLYEAVDMVINERFKKIQGRKA
                     IVLFTDGVDTTSRRADAMKNTSSALELDALIYPIQYDTYNDVQAIKNKPIITQPNPTG
                     LPLPPTNPNPSPFPFPLPGSGGGIGTPSSQGTDAADYRKAGEYLSELAARTGGRVHQA
                     NTTANLALAFSNIAAELRQLYSLGYYPKDEGKTGQRRKIKVRSNKQGLVVKARDSYIV
                     GKKEKKS"
     gene            complement(6338..6622)
                     /locus_tag="VGB68_09810"
     CDS             complement(6338..6622)
                     /locus_tag="VGB68_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049569.1"
                     /translation="MNDEHSQLNDRHSQLNDEDSHVTDEHSHVTDEHSHVTDKDSQVT
                     DEHSQLLTEHSQPLTEDSQLLIEHSQLLIEDSHALIEHSHALIEDSHLKF"
     gene            complement(6787..8178)
                     /locus_tag="VGB68_09815"
     CDS             complement(6787..8178)
                     /locus_tag="VGB68_09815"
                     /inference="COORDINATES: protein motif:HMM:NF013395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEY0049570.1"
                     /translation="MKKRFVATVSVVIALVALCAFVGQFGVVSSQSSTLLNEGFEAGG
                     KAAYAAANVTLGSGSWYLDEALTGNTTSDRKTGSWSARVRDLGKVRMNFNAASAGTVT
                     VQHAKYGSDASSAWELWKSTNGGSTWTKVGSTVTTASTTLSTASFAVNEAGAIRFEIR
                     KVSGGSNRINIDAINVASYGIATPTPTPPVSTSVHLTMGNPSGALPDSGFPTNYLMEK
                     SQYAMSYHRDDGRPNWVAWHLDTSWLGSTARQDDFRADTTLPAGWYRVTATDYSGSGY
                     DRGHMCPSADRTKTVADNSSTFLMTNMIPQAPDNNQQAWANLETYGRTLAAGGSELYI
                     ISGGQGTTGFIANGRVAVPAYTWKVIMVLPGGIDDVARVTGSTRLIAVWMPNQNGIDS
                     DWRAFRVSVDYVESMTGYDFFANVPDAVESQIESAIDNQLIEDWEMMPDKASIEEALA
                     ESSDIKTEAADRR"
     gene            complement(8558..8980)
                     /locus_tag="VGB68_09820"
     CDS             complement(8558..8980)
                     /locus_tag="VGB68_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049571.1"
                     /translation="MGYHFSSSSKEPKTKKEKLEAAKQYFVTALIFGAVTLAFAGWAY
                     RHQKEQAELSRAQAERPKVNDELFRDLARLQGEAQQKPSPVKEPPTIKSYGLSDVTPY
                     IPPIVCAAVSLLMFSMGMLYTVEARRMTETPENQERRT"
     gene            complement(9136..10452)
                     /locus_tag="VGB68_09825"
     CDS             complement(9136..10452)
                     /locus_tag="VGB68_09825"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF002772.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEY0049572.1"
                     /translation="MAQYDNVVAVILGGGAGTRLFPLTQHRSKPAVPLGGKYRLIDVP
                     VSNCINSGVTQIFVLTQYNSASLNRHISRTYRFSSFSTGFVEVLAAEQRKDSPDWFQG
                     TADAVRQTLPHLRDWRIDTLLILSGDHLYRMDYRKFLQRHFDTKANLTVSVIPSRRED
                     AEEFGLLKTDEVGKITEFKEKPTGEALEAMRVDTTNFGLSAEEAQARPFLASMGIYVF
                     NYKYLVDLLNRDASWVDFGREIIPAAIKEANVQAHLFQDYWEDIGTIRAFYEANLDLA
                     SPLPKFNFFNTDAPIYTRSRYLPPSKVHDCDIDNSMVSEGCILNGVQARRSIIGLRSR
                     IDHGVKLENAIVMGADFFESFEEMKYNFERGIPHIGIGENSVVRRAIIDKNARIGKNV
                     QLVNARGVEDDRAEENRAKDVERNYYIREGIIIVPKNATIPDGTIV"
     regulatory      10718..10913
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            10982..>11515
                     /locus_tag="VGB68_09830"
     CDS             10982..>11515
                     /locus_tag="VGB68_09830"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HEY0049573.1"
                     /translation="MPAGLDVSRLEGRVFILEKTRKNLRFRFRPLRLCAKYPARNFAS
                     VLLLFCFLAFAACKGGQANTAGAPAREMTDDLGRKVKLPLKIERAVSLAPNLTENIFA
                     VGAGDRLIGVTTFCNYPAEAEKIPKIGDTMAPNMEAIIALQPQIVFVSTASQIESFTK
                     TLEQQNINVFVTDPKDLE"
BASE COUNT         2804 a         3025 c         2979 g         2707 t
ORIGIN      
        1 gcaaacatca gaaaagcttt aatctgcaaa gcgatttcgc gccctttcga tttttccgca
       61 aaacgcacga cataatccgc cgttaaaatc aaagctcccg gcagcgccgg caaaatataa
      121 ccgggcagct tcgagccgga aaacgaaaaa aacacgagcg gagctaaaat ccacgccagc
      181 gcgaaagtgt tcagcgcgga gggctgagcg cagagctttc cgttttctcc gcgcgtctcc
      241 gcgttttctc cgcgctgaaa aaatcctttc aaaagactcc caatcgaagc cgcaaaaaac
      301 ggcagccacg gaatcgtcat caacggcagc acgagccaga aaaaccagaa ccactgcgag
      361 tgctggtatt tgttggaagt gtagcgctgg aaatgatgct gaacgaaaaa ctcgtcgata
      421 aacgtccagc cctgcgctcg atacatcggc agataccagg cgcaagcgac gagcaggctc
      481 aaaatcgtgc cccacaataa actgaaaacg aatgttttgc ccggcgattt aaatgataaa
      541 agataataaa acgcgacgac ggcaaaagga atgacgatgc cgaccaagcc cttcgccagc
      601 agcgcgacgc cgataaaaaa ataaaacgaa attaaagccg ccgagcccgc aaagattttc
      661 gagcggcggc gctctctctg tatttcttcg tgttccgggc ggctgttttc ccagatgaaa
      721 aagctgacga gcgaagccgt catcgggaaa gtcagaataa tatccagact cgccgcgcgc
      781 gagaaaacca gcaaaccgat tgacgaggcg gcaatcaaag ccagccagtt tgccaaatcg
      841 attttcggac ttctgacttc cgtctttgta ttttctctgt tttgctcctg gttcgcggca
      901 attgaaaact ttgctaaaat ccaaagacaa aaaatcgttc ccagaccgaa cagcgccgag
      961 ccgaaacgcg ccgcaaattc cgtgacgctg aagattttat aagcggcgat tgccagccag
     1021 tatagcagcg gcggtttttc gagccattga aagccgccga gcgtcggcat aatccagtcg
     1081 ccgcgctcga acatctcgcg cgcaacctgc gcgtagcgcg cctcgtcggg accgagaagc
     1141 ggaacgctca gcccgaaaaa gtagataaaa atgacggcaa ttgtgaggag aatccaaatc
     1201 aatttgtccg ttgtctgtgg ttcgttgtcg gttgccattt gtcgtttgcc gaactttaaa
     1261 agaaaataaa gtatcgctga ttaaaggttt gcattcaact gcaatccgac agcgtcggag
     1321 caaaggacaa aggacaaagg acgaatgacc aaactcagaa ccgccgttat cggcgtcggc
     1381 agtctcggca ggcatcacgc gcgcaattac gccgcgtttg ccaaagaagg cgcagcggaa
     1441 tttgtcggcg tttgcgacgc cggcgaggaa accgcaaaac aggttgccgc cgaaaacggc
     1501 tgtgattttt ttaccgattg gcgcgcgctg cttgaacagg tcgaagcggt ttcaatcgtc
     1561 acgccgaccg agacgcattc ggaaatcgcc tgcgcttttc tcgaaaaagg cgttcacgtt
     1621 ctggtcgaaa aaccgattgc gcgaactctt tccgaagccg attcgatgat aaaagccgcc
     1681 gaaaaatccg gcgcgaaatt gatggtcggg catctggaaa gatacaaccc ggcgatggtc
     1741 gcgctgcgcc cgcacgtcgc cggtccgctt tatttcgaga ttcatcgcgt cagccctttt
     1801 ccgaatcgct cgctcgacgt cgatgtcgtg ctcgacgtga tgattcacga tcttgacgcg
     1861 attcaatggc tggtcggcga aaacgtcagg gtcgcggcga ttcacgccgt cgggattccg
     1921 gtcatttccg acaaggtcga cgcggcaaac gcccgcatcg agtttgaaaa cggcgcggtc
     1981 gccaacatca cggcttcgcg catcggcacg gaaaaaattc gcaaaacgcg cttttatcaa
     2041 acaaattctt atgtcgttct ggattacgcg acgaaattcg cttccgtcac gtcgctcgcg
     2101 ccgaacgcga ttcacccgct gctcggagtt tcgataaatc gcctcgaaat caaagacgtc
     2161 gagccgctgc gcgccgaaat cggagcgttt ctcgattcaa tcgaaaacga cgaaacgccg
     2221 ccggtcacgg ctcaggacgg caggcgcgcg ttgagtctgg cgctgggcgt tctggaaaag
     2281 attgacgctc acgcgaggaa agtttttgcc gtatgaaatc cgtcgaaatt tcagcatttt
     2341 tacaatcccg catcgaggcg aaagattttc cgtccgccgt ttatctggcg gcggaaaagg
     2401 gcgaaatcgt tttgcgcgac gcggtcggct tggcggtcgt cgaaccggaa aaaatccggg
     2461 cgcggctcga tacgatttac gatttggcga gcttgacgaa ggttttgatt accggtctgc
     2521 tctgcgcgaa attgattgag acgggcgaaa taaatttgtc ggataaagcc gcgaaatatt
     2581 ttgcggaatt cgacacggac gaaaaacgcg gaataacact tggaaattta ttgacgcata
     2641 catcgggatt tcccgcctgg tttccgtttt atacgtcttt ttgtgataaa aatcgaaaaa
     2701 aggaatgtgt cctgtccgtt atagttcaaa gttcgcttgg aaatccgatt aattcaaaag
     2761 tcgtctacag cgactttaat tttctgcttc tgggtttttt gctggaaaaa ttttacggcg
     2821 agagcctgga taaattagcc gaaagggaaa ttttcgcgcc gctcggcttg caaaatactt
     2881 tttataatcc gccgaaagaa ttacgcgaaa gaatcgccgc cagcgaaaag ggaaacgaat
     2941 acgaaaaaca aacctgcgtt aattccggtt tcgacgtttc caattataat tggcgcgatt
     3001 atcaaatccg gggcgaagtt cacgacggga attgttattt tatggacggc gtttccggtc
     3061 acgccggact tttttcgacg gctgaggaag ttttcaaaat tgcgcggcag tttttacccg
     3121 aatcgacgac gcttttgcag ccggaaacct gcgcgctgtt tcgcacgaat ttcacggaag
     3181 gcttaaacga agcgcgttcg gtcgctttcc agcttgccga aacgccggat tccaccgcgg
     3241 gcgaagcgtt gtcgagaaac agtttcgggc atctgggctt taccggcaca agtctctgga
     3301 ttgacccgca aaaggaaaga atttttatac tgctgacgaa tcggacgcat aatcgtcgat
     3361 tgccgttcgt gcttctcaat tcggtcagac gaagatttca cgaatcagca gcgcgacaat
     3421 tgaacgaggc agagaccgga agtttgcaat attaagacca aacggaagtt tcggacatct
     3481 aaatattagg aaccataatc aaaacgaacg cgttttctct tccctaaaga acgccaatcc
     3541 gcctggagat tctgaaaatg agaaaaatct tatttgtttt gctgtgtgca agtgtcttta
     3601 gccttaacgc tttggcacaa acaaccattc cttcacgccc gcgcatcaat ccgtcgccgt
     3661 cgccgacgcc tgccgattcg caaacgcaaa atccgagtcc gaatcccgac agaaaccgcc
     3721 cgccggtttt aatcggcggc gagaggcagc aaacgaatcc cgtgccgacc tcgacgccga
     3781 agcccggttc gacgccgacc ggcggcgaag acgtaatcga agaagacgac gtgattcgcg
     3841 tggaaaccaa tctggtgacg attccggttg ccgtgctcga tcgcgacggt cggttcgtct
     3901 ccggtttgcg acagcaggat tttcagattt ttgaagacgg cgtgccgcag aaaatcgaaa
     3961 atttcgcttc cgtcgaacag ccttttacgg tcgttttgct gattgacgtg agcccttcga
     4021 cgcagtataa aatcaccgag attcaggatg cggcaatcgc tttcgtcaat cagctcagac
     4081 aaaacgaccg cgtcgtggtc atctcttttg acgagcgcgt tcacgtttta agtccggtaa
     4141 cgaacaaccg cgcgattttg cggaacgcga ttttgcaggc ggatttcggc aatggaacga
     4201 gcctctatga agcggtcggc gatgcgctta atcgtcaatt aagacagatc gagggacgca
     4261 aggcgattgt tttattcacc gacggcgtcg atacgacttc gagccgcgcc agctacgaaa
     4321 gcacaatccg cgacgccgaa gaatcggaag cgctgattta cccgattcgc tacgatacct
     4381 acagcgatat gggcggcggt caatcgagcg gcggcggtta cggctatccg cggcggcgtc
     4441 gcggcaacag aagactcggc gatattctgg gcgatattct gagcggcgga acttacggcg
     4501 gaggttatcc gcagggcggc ggcagcgcgg gcggcagccg tgaagattac gtcaggggca
     4561 cgcgctatct ggaagaactg gcgcgaaatt cgggcggcag aatttttcag gcgactaccg
     4621 ccgccaatct cgacgccgct ttttcgggca tcgccgaaga gctcaggcga caatacacaa
     4681 tcggttatta tcccgaaaca gccgggcaag ccggacagcg caagcaggtc aaagtgcgcg
     4741 tctcgcgtcc gaacgccgtc gtccgcgcca aaaacagcta catcgtcggc ggagtcgaag
     4801 cgcagaataa tcgaacgccg accagacagc cgccgcgtat tataaacagg ctgccgtttt
     4861 aataaaggct aaaggctaaa ggctaaaggc gaaagtgagg aataaaatcc tgctttcgcc
     4921 tttttccttt ctccttttta ctttcgcctt ttaccttttt acttttgcct ttctcctttt
     4981 tacttttgcc tttttacttt cgtctttcgc gcggttgcat cttttcattg ctgcatcgca
     5041 tcattacaat agccagccaa attttaccaa agcgaaacgg agtcgaagat aatgaaaaag
     5101 tttgcggtcg ttgttttgtc tatatttttt cttttcagct cagccggaat tcttgctcaa
     5161 tcccgccgcg tgacaaccac cgacggcaac ggcaaagcca ataaacgcga gccgaagccg
     5221 gtcgagacgc ccgcgccgca ggcgactccg aacgaagaaa ccgcgccgac cgtcaccgcg
     5281 ccggcggaaa acgcggtcga agacggcgat gtcatcagcg tcaacactaa tttagtgacg
     5341 attcccgtgc gcatttccga ccgcggcaac agatttatcg ccggactgaa aaaggaagat
     5401 tttaaagttt ttgaaaacga cgtcgagcag gaaatagctt atttttccaa cgaggaacag
     5461 ccttttacgg tcgcgctcgt tttggatatg agcctttcca gcagctttaa aatcaacgag
     5521 attcaaaacg ccgccatcgc ttttacagcg cagctgcgac cgaaggacaa ggttctggtc
     5581 gtttcgtttt ccgagcagat ttacgttttg tgcgaagcga ccagcgaccg ccagcgaata
     5641 caatccgcca tccgcagctc gcgtatcgaa tccggcacga gcctctacga agcggtcgat
     5701 atggttatca acgagcgttt caaaaaaatt cagggacgca aggcgattgt tttatttact
     5761 gacggcgtcg atacgacgag ccgccgcgcc gacgcgatga aaaatacgag cagcgcgctg
     5821 gagcttgacg cgctgattta cccgattcaa tacgatacgt ataacgatgt gcaggcgata
     5881 aaaaacaagc cgataatcac gcagccgaac ccgaccgggc tgccgctgcc gccgactaat
     5941 ccgaatccgt cgccgtttcc gtttccgctg cccggctccg gcggcggcat cggaacgccg
     6001 agcagccagg gaacggacgc cgcggattat cgaaaagctg gcgagtatct gagcgagctg
     6061 gcggcgcgca cgggcggcag agttcatcaa gccaacacga cggcgaatct ggcgctcgcc
     6121 ttttcgaata tcgccgccga gctcagacag ctctacagcc tcggctatta tccgaaggac
     6181 gaaggcaaga ccggacagcg gcgcaaaatc aaagtgcggt cgaacaaaca gggattggtc
     6241 gtcaaagcgc gcgacagcta tatcgtcggt aaaaaagaga agaaaagcta gctttgcccg
     6301 ttcgattctc ttgcgggcgc ttaaatttcc cgtaatttta aaattttaga tgtgaatctt
     6361 caatcaacgc gtgtgaatgt tcgatcaacg cgtgtgaatc ttcaatcaac agctgtgaat
     6421 gttcaatcaa cagttgtgaa tcttcagtca acggttgtga atgttcagtc aacagttgtg
     6481 aatgttcatc tgtcacttgt gaatctttgt ctgttacgtg tgaatgttca tctgttacgt
     6541 gtgaatgttc atcagtcacg tgtgaatctt cgtcgttcag ttgtgaatgt ctgtcgttca
     6601 gttgtgaatg ttcgtcgttc acttgtgaat cagcgtcggc tgtgtgtgaa ttcgcgtcgt
     6661 tcagctataa atacgcgaag ctcgccaaaa atcgttacaa aaacgtcgca tcattagtgc
     6721 aaaatgccgg gcgcgagaac cgaattcgat tcccgcgccc ggcattttgc gttttgcatt
     6781 taatcgctat cttctgtccg ccgcttccgt ttttatgtcc gacgattcgg ctaacgcttc
     6841 ttcaatcgac gctttatccg gcatcatttc ccaatcttcg attagttgat tatcgattgc
     6901 cgattcgatt tgcgattcga cggcgtccgg cacgttggcg aaaaagtcgt agccggtcat
     6961 cgactcgacg taatcgacgc tgactctgaa tgcgcgccag tcgctgtcga tgccgttttg
     7021 attcggcatc cagacggcga ttaatctggt cgagcccgtg acgcgcgcga cgtcgtctat
     7081 tccgcccggc aaaaccataa ttactttcca ggtgtaagcc ggaacggcga cgcgaccgtt
     7141 agcgataaag ccggtcgtgc cctgcccgcc ggaaatgata taaagctcgc tgccgcccgc
     7201 cgccagcgtc cgcccgtagg tttccagatt cgcccacgcc tgctggttgt tgtccggcgc
     7261 ctgcggaatc atattcgtca tcagaaacgt cgaggaattg tccgccacgg ttttcgttct
     7321 gtccgccgaa ggacacatat gcccgcggtc gtagccggaa ccggaataat ccgtcgccgt
     7381 gacgcgatac cagcctgccg gcagcgtcgt gtcggcgcga aaatcgtcct gccgcgcggt
     7441 cgagccgagc cagctcgtgt cgagatgcca cgcgacccag ttcggtctgc cgtcgtcgcg
     7501 gtgatacgac atcgcgtatt gcgatttttc cattaaataa ttggtcggaa aacctgaatc
     7561 gggaagcgcg cccgacgggt tgcccatcgt cagatgaacg ctcgtcgaaa cgggcggcgt
     7621 cggcgtcggc gtggcgatgc cgtaagacgc aacgttgatg gcgtcgatat tgatgcggtt
     7681 cgagccgccg gaaaccttgc gtatctcaaa acgaatcgcg cccgcttcgt ttaccgcaaa
     7741 gctcgccgtc gaaagcgtcg tcgaggcggt cgtgacggtc gagccgactt tcgtccacgt
     7801 cgagccgccg ttcgtcgatt tccagagctc ccacgcgctg ctcgcgtccg agccgtattt
     7861 ggcgtgctga accgtcaccg ttccggcgct cgccgcgtta aaattcatcc gcactttgcc
     7921 gaggtcgcgg acgcgagccg accacgagcc ggttttacgg tcgctcgtcg tgttgcccgt
     7981 cagcgcttcg tccagatacc agctgcccga gccgagcgtg acgttggcgg cggcgtaagc
     8041 cgctttgccg cccgcttcga aaccttcgtt taaaagcgtg gagctttgcg agctgaccac
     8101 gccgaattgc ccgacaaacg cgcagagcgc gaccagagcg atgacgacgg aaaccgtcgc
     8161 aacgaatcgt tttttcacag ttttctcctt ttatttgctt ttctgcaaga catttaaaga
     8221 ttaatgacga cgattgaatt tcgtgttaca agacgggtaa aactgttggc gagattttag
     8281 acggtttatg aaaggaaagt caataatgtt cagcgaagca gctcaggtat gcggaaagta
     8341 ttaatttaac ggtcgagatg gcgcggagag aaaccgacat acacgcgagc taagggtgac
     8401 gggcaacgcg ataacaaagc tgccgcccgc gccgtccgcg tccattgatt tgttatgctg
     8461 cctttgattg ttaaacgaaa ttatttgcct taccgtaatc ccgccaacaa atttatttcg
     8521 ataaaaataa aaaatatcac cgttgtcctt tgaattttca agttctgcgt tcttgatttt
     8581 ccggcgtttc ggtcattcga cgagcttcga cggtatataa catacccata gaaaacatca
     8641 aaagcgatac tgccgcgcaa acaatcggtg gaatataagg cgtaacatcg ctcaatccgt
     8701 agctttttat tgtcggcggc tcttttaccg gagacggctt ctgctgcgcc tctccttgta
     8761 accgcgcgag gtcgcggaac aattcatcat tgactttcgg gcgttcggct tgagctcttg
     8821 aaagctcggc ttgttctttt tgatgccggt atgcccaacc ggcgaaggct aatgtaactg
     8881 ctccgaaaat taatgcagtt acaaaatact gtttcgcggc ttctaatttt tctttttttg
     8941 tcttcggttc tttggaactg ctcgaaaaat gataacccat aataactgcc tccgaaacag
     9001 cccttaatct cgattcacat caaatcggct gtatgtcccg ataataggcg tgaaactttg
     9061 tccttccgct tcgttaatgc gacaaaatta cgggaaatgg ctcaaaatct tcaattctgc
     9121 gtcgcggtcg attcattaaa caatcgtgcc gtccggaatc gtcgcgtttt tcggaacgat
     9181 gataatgcct tcgcggatgt agtaattgcg ctcgacgtct ttggcgcggt tttcttcggc
     9241 gcggtcgtct tcgacgccgc gcgcgttgac gagctgcacg tttttgccga tgcgcgcgtt
     9301 tttgtcgata atggcgcggc ggacgacgga attttcgccg atgccgatgt gcggaatgcc
     9361 gcgctcgaaa ttatatttca tttcctcgaa cgactcgaaa aaatccgcgc ccatcacgat
     9421 ggcgttttcc agtttcacgc cgtggtcgat gcggctgcgc aagccgataa tcgagcgccg
     9481 cgcctgcacg ccgttcagga tgcagccctc gctgaccatc gaattgtcga tgtcgcagtc
     9541 gtgaactttc gacggcggaa gataacggct gcgcgtgtaa atcggcgcgt ccgtgttgaa
     9601 gaaattaaat ttcggcaagg gcgacgccaa atccagattc gcctcgtaaa aagcgcgaat
     9661 cgtcccgatg tcttcccagt aatcctgaaa aagatgcgcc tgcacgttgg cttccttaat
     9721 cgccgccggg attatctcgc gcccgaaatc gacccacgaa gcgtcgcgat tgagcagatc
     9781 gaccagatat ttataattaa aaacgtagat tcccatcgaa gccagaaacg ggcgcgcctg
     9841 cgcttcttcc gccgacaatc cgaaattcgt cgtgtccacg cgcatcgctt cgagcgcttc
     9901 accggtcggt ttttccttaa attccgtgat ttttccgact tcgtccgttt tcagcaagcc
     9961 gaattcctcg gcatcttcgc gccgcgatgg gatgacggaa acggtcaggt tggctttggt
    10021 gtcgaaatga cgctgcaaaa atttgcggta atccatccgg taaagatggt cgcccgacaa
    10081 aatcaaaagc gtgtcgatac gccagtcgcg caggtgcggc agcgtttgcc tgaccgcgtc
    10141 cgccgtgccc tgaaaccaat cggggctgtc tttgcgctgt tcggcggcga gcacttcgac
    10201 aaagccggtc gaaaagctcg aaaagcgata cgtgcgcgag atgtggcgat tcagcgaagc
    10261 cgagttatac tgcgtcaaaa caaaaatctg cgtgacgccc gaattgatgc agttcgatac
    10321 cggaacgtca atcagacgat attttccgcc gagcggaacc gccggtttcg agcgatgctg
    10381 cgtcagggga aaaagccgcg tgcccgcgcc gccgcctaag ataaccgcca ccacgttgtc
    10441 gtattgcgcc ataaaattaa aaagggaatc gatttgaaaa tactttattt cagaatagcc
    10501 ataagcgttt gtgatttcaa gaggtttgag gagatttagg attcaggatt caggattcag
    10561 gattcaggat tgaaagaaag ttcccaatcc tgaatcctga atcctgaatc ctgaatttaa
    10621 attgcatttt tgacattccg ccgctcgccg tgctaacttg cctttcattc gggtcagcaa
    10681 attcgcgtat gctttcagag ccggattcgt gttcttttta tcgtaaaacg cttcgggtta
    10741 tcgctcttcc gaaaggagaa gcgaattaag gggaagtcga gtgaaaatct cgcacggtcg
    10801 cgccacagtg aaaaggataa aggataaagg ataaataaag catcttattg cttatttcgc
    10861 ctttcgcctt tcgcctttaa gtctggagac ctgcccgcag cagttttgtg ccgttgaata
    10921 tctcgcgtca agatgttcgg gctgttcggg tcgtttttac ttcttttttt agtgaaaaac
    10981 cttgccggct ggtcttgatg tttcgagact ggaaggcagg gtttttattt tggaaaagac
    11041 gaggaaaaat ctacggtttc gctttaggcc tttgcgcctt tgcgcgaaat atccggcgcg
    11101 aaatttcgcg agcgttttgc tgctcttttg ctttctcgct ttcgccgcct gcaaaggcgg
    11161 gcaggcaaat accgccggcg cgccggcgcg cgaaatgacc gatgatttag gacgaaaagt
    11221 aaaacttcct ctgaaaatcg agcgcgccgt atccttagcg ccgaacctga cggaaaacat
    11281 tttcgccgtc ggcgcgggcg accgtctaat cggcgtgacg accttctgca attatcccgc
    11341 cgaagccgaa aaaatcccga aaatcggcga tacgatggct ccgaatatgg aagcgattat
    11401 cgctctccag ccgcaaatcg tttttgtttc gaccgcttcg caaatcgaat cttttaccaa
    11461 aacgctcgaa cagcaaaaca tcaacgtttt cgtcaccgac ccgaaagatt tggaa
//
LOCUS       DASWNE010000497         4717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_509294, whole genome shotgun sequence.
ACCESSION   DASWNE010000497 DASWNE010000000
VERSION     DASWNE010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509294"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="VGB68_09835"
     CDS             <1..1009
                     /locus_tag="VGB68_09835"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0049574.1"
                     /translation="GDIITVGNDPSAVIELDEEGILPEQFVIINEHGQPLLLCRTDGI
                     LVNDRFLQQGSLHELQHEDKILVGNYSLQYDAEDAFNLSENGDEIEFSPSAITEDLQP
                     PETQPGNIDENGDIHKTNALNREQTLTDILENLRAEEKYYFQIEKIRGENRRAYVESE
                     NVWLGWSGAGECVIGALNTVETPKAQIRKDWTGVILYPHGGQAVWLNNEILNEPTRLK
                     NDDRILLSGQTPTEPERSTLIRFHEPTALLVLDSILPKELPPPVSLETAQSGKAQETA
                     RRNIAAAQETTALQANPPAPKPKRAARVLGYFTWAEILIMIIGTLVTAAVIFLVLELS
                     Y"
     gene            1250..3442
                     /gene="sctV"
                     /locus_tag="VGB68_09840"
     CDS             1250..3442
                     /gene="sctV"
                     /locus_tag="VGB68_09840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctV"
                     /protein_id="HEY0049575.1"
                     /translation="MKAIGNTFDFNSLLRQGNITGLMARYKNLLLPIGLLLVVGTLFI
                     PLHPELVSGLILVNLAISITVLVTALFINSPVQLTSYPSILLLTTIFRLTLSVSVTRN
                     ILSHGEPGSVVEALGHVTAQGNLITGAVLFIIILVVQFLVVSKGSERVAEVAARFTLD
                     AMPGKQMAIDADLRSGLINQDQARHMRTELQKESQLHGAMDGAMKFVKGDVICTIIIA
                     LVNIVAGLAAGVMMHGLSFSEAANKYTIATFGDGLAAVISSLLVTLSAGIVVTRVSTG
                     EDSNIGSDIGEQLFSSPRPLFITGGIMLLLTPLNLLFVPVGAGVAGLGYLLHRRQKRI
                     ERESDADNAPHSGGDSEELQMSFTVPMAVVVSRELTHLVDKNTPQGAKFRAEIPKLRN
                     SLYYDLGVMVPFCYVGGDAPIAPNEYYIAVKEVPVGTGSLRPNCVYVNDSAENIRVFG
                     IDGENVSNPADLRPGAWIPADQRHIAETAGLKVWEPGDVIILHLSRVMKRYAHEFIGI
                     QESQGYLDFIARGMPKLVEEVVPKVVSIHQFTDVLQRLVQEGISVRDTKSILDALSEW
                     GRIEKDPVMLTEHIRTAMRRHISFRYAAGSDTLFVYLLDPEIEDVIRGAIRRTSTGSF
                     LSLDPTIAHDILDAIRQEIVEQPPTAQPPVIVTDMELRRFVRKMVELEFQNLAVLSYQ
                     ELTPELNIQPIGRITMRSARLENDFEAGFYGDFAELPGASLEVDEFKS"
     gene            3485..4333
                     /locus_tag="VGB68_09845"
     CDS             3485..4333
                     /locus_tag="VGB68_09845"
                     /inference="COORDINATES: protein motif:HMM:NF013664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049576.1"
                     /translation="MQIRFFKRQGFIRQLIFGSLLAVLLLSGCSLRKKVQSVKIEAEA
                     NQIIDVLRENGVRARKEEIGEGERKTFEIWVDGDEDDLGAAIQVMEDHCLGQPEPPAI
                     ESSGIISSVEVEKAREQRRMKINIESQLRQIPGTTCVNVNFVPPQDRALAINPYPATA
                     AVVVNFKTPTFSVPPEQIAGIVARSVPDLKPENVSVVLNSKPLRPLPENMGSRPFTRI
                     ALVTGIGLATILAFISIVFFLQKKRRGQDDELENDFVAAPAPNPKGETSLLDEGDEFD
                     DDIKFP"
     gene            4383..>4717
                     /locus_tag="VGB68_09850"
     CDS             4383..>4717
                     /locus_tag="VGB68_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049577.1"
                     /translation="MIPTISQSERLDILLALALVFRRTEEDGGQSAAQLAEKSFSALS
                     PKDLETLQIRAGLFDRLPPKNQQLWQSFWLDKIRRRGRSVRLDERINPLQMAEILRSE
                     PQAIQLLILR"
BASE COUNT         1092 a         1271 c         1306 g         1048 t
ORIGIN      
        1 cggcgacatc atcacggtcg gcaacgaccc gtcagccgtt atcgagctcg acgaggaagg
       61 aatcctgccc gaacaattcg tcattatcaa cgaacacggt caaccgctgc ttctgtgccg
      121 caccgacgga attctcgtca acgaccgctt tttgcagcag ggctcgctcc acgaattgca
      181 gcacgaagac aaaattctgg ttggaaatta ctctctgcaa tacgacgccg aagacgcatt
      241 taatttatcg gaaaacggcg acgaaatcga attttcgccg tcggcgataa ccgaagattt
      301 gcagccgccg gaaacgcagc ccggtaacat cgacgaaaac ggcgatattc acaaaaccaa
      361 cgccctgaat cgcgagcaga ctttgaccga cattctggaa aatctgcggg cggaagaaaa
      421 atattatttt caaatcgaaa agattcgcgg cgaaaaccgg cgcgcttacg tcgaaagcga
      481 aaacgtgtgg ctcggctggt caggcgcggg cgagtgcgtt atcggcgcgc tgaacacggt
      541 cgaaacgcca aaagcgcaaa ttcgcaaaga ctggacgggc gtgattttgt atccgcacgg
      601 cggtcaagcc gtctggctca acaacgaaat tctaaacgag ccgacgcgtc tgaaaaacga
      661 cgaccgcata ctgctttcgg gacagacgcc gaccgagccg gagcgctcga cgctgatcag
      721 attccacgag ccgaccgcgc ttctggtgct cgattcgatt ttgccgaaag agctgccgcc
      781 gccggtttcg ctggaaacgg cgcaaagcgg aaaagctcag gaaacggcgc gcagaaatat
      841 cgccgccgcc caggaaacca ccgccttaca ggcgaatccg cccgcgccga aaccgaaacg
      901 cgccgcgcgt gttttaggtt atttcacctg ggcggaaatc ctgattatga ttatcggcac
      961 gctggttacg gcggcggtga tttttctggt tctggaattg tcttattaaa ttaaattaaa
     1021 tgatgaaaaa agagatgcgg gcgcacgccg cagcgcccgc agatggattg ttgacgcggc
     1081 accttttttt ttcaggctaa gcccggctct cacaaacttg caaacaaccc ggacagggat
     1141 tttcgactca caatcaatta cactaaaacc ggcaaactta aaaattattt tacaaatcgc
     1201 gctttgctgt taaaattaat cttatcttga gcagacagaa aatttattaa tgaaagccat
     1261 cggtaacacg tttgatttca attcgctgct ccgtcagggc aatattacgg ggcttatggc
     1321 aaggtataaa aatctgcttt tgccaatcgg tttattgctg gttgtcggca cgctttttat
     1381 tccgcttcat ccggaactgg tttccgggct gattctcgtt aacctcgcga tttcgattac
     1441 ggttctggtg acggcgctgt ttatcaactc gcccgtgcag ctgacctcct atccttcgat
     1501 tttactttta acgacgattt ttcgtttgac tctgagcgtt tccgtaacgc gcaatatttt
     1561 atcgcacggc gagccgggct cggtcgtcga agcgctcgga cacgtcacgg cgcagggaaa
     1621 cctaatcacc ggcgcggtgc tttttatcat tattctggtc gtccagtttc tcgtcgtcag
     1681 caaaggctcg gagcgcgtcg ccgaagtcgc cgcgcgtttt actctggacg cgatgcccgg
     1741 caagcagatg gcgattgacg ccgatttgcg ttcgggtttg attaatcaag accaggcgcg
     1801 ccatatgcgc accgaattgc agaaggaatc gcagctgcac ggcgcgatgg acggcgcgat
     1861 gaaattcgtc aagggcgacg tcatctgcac gattatcatc gcgctggtca acatcgtcgc
     1921 cgggctggcg gcgggcgtaa tgatgcacgg actttcattt tccgaagcgg cgaacaaata
     1981 cacgattgcc actttcggcg acggcttggc ggcggttatt tcctcgctgc tggtgacgct
     2041 ttccgccggt atcgtcgtca cgcgcgtttc gacgggcgaa gattcgaaca tcggctcgga
     2101 catcggcgag cagctttttt cttcgcccag accgcttttt atcacgggcg gaattatgct
     2161 tctgctgacg cctctgaatc ttcttttcgt tccggtcggc gcgggcgtgg cgggactcgg
     2221 gtatctgctt caccggcggc aaaaacgcat cgagcgcgaa agcgacgcgg ataacgcgcc
     2281 gcacagcggc ggcgattccg aagaattgca gatgagcttt accgtgccga tggcggtcgt
     2341 cgtcagccgc gagctgactc atctggtcga taaaaacacg ccgcagggcg cgaaattccg
     2401 cgccgaaatt ccgaagctgc gcaattcgct gtattacgat ttgggcgtga tggttccgtt
     2461 ctgctacgtc ggcggcgacg cgccgattgc gccgaacgaa tattacatcg ccgtcaagga
     2521 agttccggtc ggaacgggca gcttgcgccc gaattgcgtt tacgtcaacg attcggcgga
     2581 aaacatccgg gttttcggca tcgacggcga aaacgtcagc aatccggcgg atttgcgacc
     2641 gggcgcgtgg attcccgccg accagcgtca catcgccgaa accgccgggc tgaaagtctg
     2701 ggagccgggc gacgtgatta tcctgcatct ttcgcgcgtg atgaaacgtt acgcgcacga
     2761 atttatcggc attcaggaat cgcagggcta tctggatttt atcgcgcgcg gaatgccgaa
     2821 gctggtcgag gaagtcgtgc cgaaagtagt atcgattcac cagttcaccg acgttttgca
     2881 gcggcttgtg caggaaggca tttcggtgcg cgacacgaaa tccattctcg acgctctctc
     2941 ggaatgggga cgcatcgaaa aagacccggt aatgctgacc gagcacattc ggacggcgat
     3001 gcggcgacat atttcgtttc gctatgccgc cggcagcgac acgctgtttg tttatctgct
     3061 cgacccggaa atcgaagacg tgattcgcgg cgcgattcgc cgcacctcga cggggtcgtt
     3121 tttgtcgctc gacccgacca tcgcgcacga tattctcgac gccatccggc aggaaatcgt
     3181 cgaacagccg ccgaccgcgc agccgcccgt tatcgtaacc gatatggagc tgcgccgctt
     3241 tgtgcgaaaa atggttgagc tggaatttca aaacctcgcg gttttgtctt atcaggaatt
     3301 gacgccggag ctgaatattc agccgatcgg acgcatcacg atgcgctcgg cgcggctgga
     3361 aaatgatttc gaagcgggct tttacggcga ttttgccgag cttcccggtg catcgctgga
     3421 agtcgatgaa tttaaaagct gacaaatcgc ttccggcttc gctaaatgtt ttaaaattta
     3481 aataatgcaa ataagatttt tcaagcgtca agggtttatc aggcaactga ttttcggcag
     3541 cctgctggcg gttttgcttt tgagcggctg ttctttacga aaaaaagtgc agagcgtgaa
     3601 aatcgaagcc gaagccaatc agattatcga cgttctgcgc gaaaacggcg tgcgcgcgcg
     3661 caaggaagaa atcggcgaag gcgagcgcaa aactttcgag atttgggttg acggcgacga
     3721 ggacgacctc ggcgcggcga ttcaggttat ggaagaccat tgtctcggtc agcccgaacc
     3781 gcccgccatc gagtcgagcg gaattatttc gtcggtcgag gtcgaaaagg cgcgcgagca
     3841 gcggcggatg aaaatcaaca tcgaatcgca gctgcgccag attcccggca cgacctgcgt
     3901 caatgtcaat ttcgtgccgc cgcaggaccg cgcgctggcg ataaatccat atccggcgac
     3961 ggcggcggtc gtggtcaatt tcaaaacgcc gaccttttcc gtcccgccgg aacagattgc
     4021 cgggatcgtc gcccgcagcg ttcccgactt gaaaccggaa aacgtgagcg tcgttttaaa
     4081 ttccaagcct ttgcgcccgc tgcccgaaaa tatgggcagc cgcccgttta cgcgcatcgc
     4141 gctggtgacg ggcatcggtc tggcgacgat tctggctttt atttccatcg ttttcttttt
     4201 gcagaaaaaa cgccgcgggc aggacgacga gttagagaac gatttcgttg cggctcccgc
     4261 gccgaatccg aaaggcgaaa cttccctgct cgacgagggc gacgaatttg acgatgacat
     4321 aaagtttccc taaaaacgcg agaaacttaa tcaaaacgga agaaatttaa tttttaaaag
     4381 cgatgattcc gacgatttca caatccgaaa ggctcgatat tctgctggcg ctcgcgctcg
     4441 tttttcgccg gacggaagaa gacggcgggc aaagcgccgc gcagctcgcc gaaaaatcgt
     4501 tttccgcttt aagcccgaaa gatttggaaa ccttgcagat tcgcgcggga ttattcgacc
     4561 ggctgccgcc gaaaaatcaa cagctctggc aatctttctg gctggataag attcgacggc
     4621 gcggacgctc cgtcaggctt gacgaacgca tcaacccgct gcaaatggcg gaaattctgc
     4681 gaagcgagcc gcaggcgatt cagcttttga ttctgcg
//
LOCUS       DASWNE010000498         1605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_613015, whole genome shotgun sequence.
ACCESSION   DASWNE010000498 DASWNE010000000
VERSION     DASWNE010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613015"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1531
                     /locus_tag="VGB68_09855"
     CDS             <1..1531
                     /locus_tag="VGB68_09855"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0049578.1"
                     /translation="AALENLKPVEPAKRREHSASSQLLMLRLAEAVASRELLFRELVA
                     ILQQESKAKRILIAESKEQKKFYPFITQGFTSAESAELVAKAQQAHLSGNLEHFAKTK
                     NLSIFPLRAPSAPPAMLMIYPRSSANLLDGSLLQPLLRVVELGMDVCALREKDKAQQT
                     EHEVNPFTSQSLMQGFIHSSPAMTSLVEEVYKIRSSDVTVLVTGESGTGKELVSRAIH
                     TLSNRKDKVFVPFNCTAVPKELAEGHLFGYRKGAFTGAVNDSVGVIRSANGGTLFLDE
                     IGDLPLEVQPKLLRFLQEGEIHPLGEKAPIKVDVRVIAATNMPLEEKVADGSFREDLY
                     YRLNVIRLRVPPLRERRSEIPPIVNYYLNQYSARFGKRDLNMTPQTMDLLMVCEWEGN
                     VRQLCNEVQRIVARAVDGETITPEHLSHDLKRSAVPIPSFAAAGNIASISSFNASSFN
                     IANNGGTLEEAVSELEKRMIREAMRRHGNNISRVARELGLTRRGLYLKLGRYQLVDKA
                     S"
BASE COUNT          369 a          450 c          444 g          342 t
ORIGIN      
        1 agccgcgctg gaaaatctga aaccggtcga gccggcgaaa agacgcgagc attccgccag
       61 ctcgcagctg ctgatgctcc ggctggcgga agcggttgcc tcgcgcgagc ttcttttccg
      121 cgagctggtc gccattctcc agcaggaaag caaagccaaa cgcatcctga ttgccgaatc
      181 gaaagagcag aaaaaatttt accctttcat tacgcaggga tttacctccg ccgaaagcgc
      241 ggagctggtc gccaaagcgc agcaggcgca tttgagcggc aatctcgaac atttcgccaa
      301 aacgaaaaac ctgagcattt ttccgcttcg cgcgccgtct gccccgcccg cgatgctgat
      361 gatttacccg cgttcgagcg cgaatctttt ggacggaagc ctgctccagc cgcttctgag
      421 agtggtcgag ctcggaatgg acgtttgcgc tctgcgcgaa aaagacaagg cgcagcagac
      481 cgagcacgaa gtcaacccgt ttacttcgca gagcttgatg cagggcttta ttcattccag
      541 cccggcgatg acttcgctcg tcgaagaagt ttataaaatt cgctcgtcgg acgtgaccgt
      601 tctggtcacg ggcgagtcgg gcacgggcaa agagcttgtt tcgcgcgcga ttcacacgct
      661 atcgaaccgc aaagataagg ttttcgtgcc gtttaactgc accgccgtgc cgaaagagct
      721 ggcggaaggt catctgttcg gctatcgcaa aggcgcgttt accggcgcgg tcaacgattc
      781 ggtcggcgtc attcgctcgg caaacggcgg aacgcttttt ctggatgaaa tcggcgatct
      841 gcctctggaa gtccagccga agcttctgcg atttttgcag gaaggcgaaa ttcacccgct
      901 cggcgaaaaa gcgccgataa aagtcgatgt ccgcgtgatc gccgcgacca atatgcctct
      961 ggaagaaaag gtcgccgacg gcagcttccg cgaagatttg tattatcgcc tgaacgtgat
     1021 tcgcctgcgc gtgccgcctc tgcgcgagcg gcgctcggag attccgccga ttgtcaatta
     1081 ttatctcaac cagtattcgg cgcgtttcgg caagcgcgat ttgaatatga cgccgcagac
     1141 gatggatttg ctgatggtct gcgaatggga aggcaacgtg cgccagcttt gcaacgaagt
     1201 tcaacgcatc gtcgcgcgcg ccgtcgacgg cgagacgatt acgcccgaac atctttcgca
     1261 cgacctgaaa cgcagcgccg tcccgattcc gtctttcgcc gccgcgggaa acatcgcgtc
     1321 gattagctct ttcaacgctt cttcgttcaa tatcgccaac aacggcggaa cgctcgaaga
     1381 agccgtttcg gagctcgaaa aacgaatgat acgcgaggcg atgcgccgcc acggcaacaa
     1441 tatctcgcgc gtcgctcgcg agctgggtct gacgcgtcgc ggactttatc tcaaattagg
     1501 acgctatcaa ctggtagata aagcaagtta gataaattca ggattcagga ttcaggattc
     1561 aggattggaa atcgaatcct atcttaaatc ttaaatcctg aatcc
//
LOCUS       DASWNE010000499         6346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_264708, whole genome shotgun sequence.
ACCESSION   DASWNE010000499 DASWNE010000000
VERSION     DASWNE010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6346
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264708"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="VGB68_09860"
     CDS             complement(<1..862)
                     /locus_tag="VGB68_09860"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049579.1"
                     /translation="MKNALFVFAVLTGIIISGGSVAIVAQTDMKPPVAKKEPKVLKIH
                     GYEIVDDYAWLRDRNDKKNPEIMKYLEAENAYTESFMKPHQPFIDNLYKEMLGRIKQD
                     DTSVPYKLGDYWYFNRTEEGKQYPVYLRSRTKDGKDAQVLLDQNEMAKGFKYFAIGDF
                     AVSDDGNILAFSTDTTGFRQYTLQFKDLRTGKILPDRIERATSAAWATDSKTIFVVTE
                     DEETKRSDKFWRHAVGTNKNELVSEEKDELFFFAVNRTRDKKMIMLQSYAATMREFRY
                     LPADQPAGEWK"
     gene            complement(1218..2372)
                     /locus_tag="VGB68_09865"
     CDS             complement(1218..2372)
                     /locus_tag="VGB68_09865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEY0049580.1"
                     /translation="METGKDSTAAAASAASAGLRGVVAAQSSIGDVNGEKGILIYQGY
                     DIHDLAEHSNFEEVVYLLWNGRLPAKSELEDLREQLRAHYEAPAEVIEFMRQFPKEAD
                     PMDVLRTAVSALDCYDTGGHGTDRENAVRTAIKLTAQIGTLVAAWERIRNGQDVIAPD
                     KSLGIAENFLYMLRGEKADADEARMFDICLILHADHELNASTFTTRVIAGTLADVYGA
                     VTGGIAALAGPLHGGANTAVMKTLLEVGAVENVEPWLDKALAEKRKVMGIGHAVYKTE
                     DPRATWLRKFSRQMGERKGEMKWFEMSQKLEKLMLDKKGMYPNVDFYSASTYYLMDIP
                     LDLYTPIFALSRISGWTGHILEQYGNNKLIRPRAEYIGERDLKYTSIDER"
     gene            complement(2462..3274)
                     /locus_tag="VGB68_09870"
     CDS             complement(2462..3274)
                     /locus_tag="VGB68_09870"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEY0049581.1"
                     /translation="MGVITLLTDFGTRDYFVGAMKGAILSVNPQASVIDITHEIPPHD
                     ISSAAFTLANYYKNFPGGTIHVCVVDPGVGSARRAILLETEDYFFIAPDNGLLSFAFD
                     KIESGKSRRGLAPRRFRVFELTNERYFAAAVSGTFHGRDVFAPVAGHLSGGVEPEKFG
                     DEIEDFIRFEMPQPRNLSAGEIEAEIIHIDYFGNLITNLKAEDLPAEFFLEVNETRVE
                     RHQKFYAEAEKGEIFSIAGSAGFLEIVAFQDSAKRLLKAEAHQKFLLLKAKE"
     gene            complement(3379..4419)
                     /locus_tag="VGB68_09875"
     CDS             complement(3379..4419)
                     /locus_tag="VGB68_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049582.1"
                     /translation="MKLTRNLLAVAISLSLVFISSLNLPIQPIAGAQQDTRAALQRGY
                     RTGYSDGYMAGYRDVIENAKRNFQRHSEYEKADRAFSKDYGALEDYQDGYRQGFENGY
                     NTGFDKRSFDASLPPELKRRGNVQTDTTAEVIENTEPENPPAIVDKTPEQNTEVTTTA
                     VVPVDERSVIIPVETELIIELVEGIDTEQSRIGDKFQARVVSPAEISGALIEGRISKI
                     QKPGRIKRRAEMQLSFDRINLGDNRWGNYNAILTEVLPLKGDGVRRVDVEGTVEGKRN
                     YKKDAAKLGASTGGGAVVGGLIGGPVGVAVGAGVGAAFGVGAVVVERGKHIKLIRGQQ
                     LRIKTAYETQIR"
     gene            5074..5607
                     /locus_tag="VGB68_09880"
     CDS             5074..5607
                     /locus_tag="VGB68_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049583.1"
                     /translation="MRRNNTANNFWRVFLAFALGVFVFANYAEAQTKRPQQKPAEQIT
                     KQMKDEVTYEPDGGLLKEMWSRISVVESKKDWKQFKRQRPTLNKSGSRSAYIAQFAIE
                     PGGEALIDVIVVYEKSSGKFYELRGFDFPRPFVELKWTSDDVLQFEQWVNPTNGGRYA
                     INVKRGEIVSAGYVRSN"
     gene            5625..6218
                     /locus_tag="VGB68_09885"
     CDS             5625..6218
                     /locus_tag="VGB68_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049584.1"
                     /translation="MKKRILSIAILILISASAGFAQTIEQQVIRIRNLYAATNKRIDE
                     GLKDKISGLHYAARTIGGERDGRQWAAVGTMKKLDEFWFDGEPDFNEEENPDARKMIR
                     KIISTYAGAADLRTRSEYLFDAETGECVFAFTTELGADGKTTERRLYFHKGKLIRIAR
                     GGKNVDANFSAADLNAAQIESDAAKRLKNLFALMMTG"
BASE COUNT         1533 a         1569 c         1550 g         1694 t
ORIGIN      
        1 ttttccattc gccggcgggc tggtcggcgg gcagatagcg aaattcgcgc atcgtcgccg
       61 cgtaagactg gagcataatc atctttttat cgcgcgtccg gttgacggca aaaaagaaaa
      121 gctcgtcctt ttcttccgac accaattcgt ttttgttcgt gccgacggcg tgccgccaga
      181 atttatcgga acgcttggtt tcttcgtcct cggtgacgac gaaaatcgtt ttactatcgg
      241 tcgcccacgc cgcgctcgtc gcgcgctcga tacggtcggg cagaattttt ccggtgcgca
      301 aatccttaaa ctgcaaggtg tattgacgaa agccggtcgt atcggtcgaa aacgccagga
      361 tattgccgtc gtcggaaacg gcaaaatcgc cgatggcaaa atatttaaag cctttcgcca
      421 tctcgttctg gtcgagcaga acctgcgcgt ctttgccgtc tttcgttcgg ctgcgcagat
      481 aaaccggata ctgtttgccc tcttcggtgc ggttaaaata ccagtaatcg ccgagcttgt
      541 acggcacgct cgtatcgtcc tgcttgatgc gccccagcat ttccttgtaa agattgtcga
      601 taaaaggctg atgcggcttc ataaagctct cggtgtaagc gttttccgct tcgagatact
      661 tcataatctc gggatttttc ttgtcgtttc tatcgcgcag ccacgcgtaa tcgtcgacga
      721 tttcgtagcc gtgaatcttc aaaactttcg gttctttttt tgcaacaggt ggtttcatat
      781 cagtctgggc aacaatcgca acgctgccgc cggaaataat tatgcccgtt aaaacggcaa
      841 acacaaaaag cgcgtttttc atttttgtaa tctccaatcc cgattttttt cgccgggaga
      901 aatgatttga tgttctgaga tattttaacg gaatttaatt cgcagtaaag ccatttgcag
      961 attcaatcag atttttcttt ttgcgctcac agattgtgca aatcgaaagg cgcgcatctt
     1021 ttttaaaaaa agcgcgcgga aattattcga caatcataac cgccctgatt cccgcgtgaa
     1081 tgtccgtgat tttgagaatc cgcgattcgt ttttcgtttg ccggataatt accaatcata
     1141 attttcgata aataaaaaag gcaaaacgaa aagccttaac tttcctttgc ctttttgctt
     1201 tttgcttttg ccttcgctta tctttcgtcg atcgacgtgt atttcaaatc gcgttcgccg
     1261 atgtattcgg cgcgcgggcg aatcagcttg ttgttgccgt attgttcgag aatgtgtccc
     1321 gtccagccgg atatgcgcga gagagcgaaa atcggcgtgt agaggtcgag cggaatgtcc
     1381 atcaggtaat aagtcgaagc cgaataaaaa tcgacgttcg gatacattcc ttttttatcg
     1441 agcatcagct tttcgagctt ttgcgacatt tcaaaccatt tcatctcgcc tttgcgctcg
     1501 cccatctggc gcgagaattt gcgcagccaa gtggcgcgcg ggtcttcggt tttgtaaacg
     1561 gcgtgaccga tgcccatcac cttgcgtttt tccgccaggg ctttatcgag ccagggctcg
     1621 acgttttcga ccgcgccgac ttccagaagc gttttcataa cggctgtatt cgcgccgccg
     1681 tgcagcggtc cagccagagc cgcgatgccg cccgttactg ccccgtaaac gtccgccagc
     1741 gttccggcga taacgcgcgt cgtgaaagtc gaggcgttta attcgtggtc ggcgtgcaga
     1801 atcaggcaga tatcgaacat ccgcgcttcg tccgcgtcag ctttttcgcc gcgcagcata
     1861 taaaggaagt tttcggcgat gcccagggat ttgtcgggcg caattacgtc ctgaccattg
     1921 cggattcttt cccacgcggc gaccagcgtt ccgatttgcg cggtcagctt gattgccgtc
     1981 ctgacggcgt tttcgcggtc ggttccgtga ccgcctgtat cataacaatc cagcgccgaa
     2041 accgccgtgc gcagaacgtc catcgggtcg gcttctttcg gaaactgccg cataaactcg
     2101 atgacttcgg cgggcgcttc gtaatgggcg cgaagctgct cgcgcaaatc ttcgagctcg
     2161 cttttcgccg gaagcctgcc gttccagagc aggtaaacga cttcttcaaa atttgagtgt
     2221 tcggctaaat cgtgaatgtc gtaaccctga taaatcaaaa ttcccttttc gccgttgacg
     2281 tcgccgattg acgattgagc ggcgacgacg ccgcgcagcc ccgcactggc ggcggaagca
     2341 gcggcggcgg ttgaatcttt tccagtttcc atagtagttt tcccttataa atattgaatt
     2401 tattgcgcta ttttaaattt agcaaagacg ctttcaataa acaagttagt aaaaaatata
     2461 attattcctt tgccttcagc aataaaaatt tttgatgcgc ctcggctttc aacaggcgtt
     2521 tagccgaatc ctgaaacgcg acgatttcca gaaagcccgc gcttcccgca atcgaaaaga
     2581 tttctccttt ttcggcttcg gcataaaact tttgatgccg ctcgacgcgc gtttcgttga
     2641 cttcgagaaa gaattcggcg ggtaaatctt cggcttttaa attcgttatc aggttgccga
     2701 aataatcgat gtgaattatc tcggcttcga tttcgccggc ggaaagattt cgcggctgag
     2761 gcatttcaaa tctaataaaa tcctctattt cgtcgccgaa tttttccggc tcgactccgc
     2821 cggaaagatg cccggcaacc ggcgcgaaaa catcgcgccc gtgaaaagtt ccgctcactg
     2881 cggcggcaaa atatctttcg ttcgtcaatt caaaaacgcg aaaacgtcgc ggcgcgagcc
     2941 cgcgccgact ttttccgctt tcgattttat caaacgcaaa actcaataaa ccgttgtccg
     3001 gcgcgataaa aaaataatct tcggtttcca gcaaaattgc gcgcctcgcc gagccgacgc
     3061 ccgggtcgac cacgcagacg tgaatcgttc cgcccggaaa atttttataa taattggcga
     3121 gcgtgaaagc ggctgaggag atgtcgtgcg gcggaatttc gtgcgtgatg tcgataacgc
     3181 tcgcctgcgg attgacggat aaaatcgcgc ctttcatcgc gccgacaaaa taatctctgg
     3241 tgccgaaatc cgtcagcaaa gtaatcacgc ccatatttta atcaattaca aattaaaaat
     3301 tacgaattac gagttgaaga aaataatcct tctttcgtaa ttcgtaattc gtaattcgta
     3361 attcgtaatt ctaattgact acctgatttg cgtttcgtaa gcggttttaa tccgcagctg
     3421 ctgaccgcgg ataagcttaa tgtgcttgcc gcgttcgacg accaccgcgc cgactccgaa
     3481 agccgcgccg acgcccgcgc cgaccgccac gccgaccgga ccgccgatca agccgccgac
     3541 caccgcgccg ccgccggtcg aagcgccgag cttagcggcg tcctttttat aatttctttt
     3601 gccttcgacc gtgccttcga catcgacgcg tctgacgccg tcgcctttca gcggcagaac
     3661 ttcggtcaga atcgcgttgt aattgcccca gcgattgtcg cccagattga tgcggtcaaa
     3721 ggaaagctgc atctcggcgc ggcgtttgat tctgccgggt ttttgaattt ttgagattct
     3781 gccttcgatt aaagcgccgc tgatttcggc gggcgaaacg acgcgcgcct gaaatttgtc
     3841 gccgattcgg ctctgctcgg tgtcgatgcc ttcgaccagc tcgataatca actcggtttc
     3901 gaccggaata atcacgctgc gctcgtcaac cggcacgacc gccgtcgtcg taacctccgt
     3961 gttttgttcc ggcgttttgt ccacgattgc cggcggattt tccggttcgg tattttcaat
     4021 tacttccgct gtcgtgtcgg tctgcacgtt tccgcgtcgt ttcagttcgg gcggcaggct
     4081 cgcgtcaaac gagcgtttat cgaatccggt gttgtagccg ttttcaaagc cctgtcgata
     4141 accgtcctga taatcttcga gcgcgccgta atcctttgaa aaggcgcgat cggctttttc
     4201 gtattcgctg tgacgctgaa aattgcgctt tgcattttca atcacgtcgc ggtagccagc
     4261 catatagccg tccgaatacc cggtgcgata accgcgctgc aatgcagccc gcgtatcctg
     4321 ttgagcgccg gctatcggct gaatcggtaa atttagggaa gagataaaaa cgagggaaag
     4381 acttatggcg acagctaata gatttctggt tagcttcatc tggaatctcc ttttcaaaaa
     4441 aacaaagaaa tgatttcaac gcttagactt atacactaaa acttaaagaa aaacaagaag
     4501 aatttccggt tttcggaaat cggcaatttg cggagctgta actgaaagca cgagctggca
     4561 gttttgccaa tttatgcttt caaaaactct ctgccagctt atgctttcag ttgtatttct
     4621 acatataaaa gcaattttga ttcgatttac tctcagatat tgaagagcaa tcaatcgcta
     4681 tgatttcgct tgtatccgct tttaacacaa gccaaattat ttattttata ataagtcggc
     4741 aattggggag atttaaaagg ttcgtcagca tcagttatac cacttttatc gctaaagatt
     4801 aaagatattt tgaaggattt atttgtgttt ttactttatt tccatttttc tcgtctctgt
     4861 aggaacaaaa ttaaaattag cgggagaacg cggcgcataa cgaaacctcg gatttgcatt
     4921 tgattataag tttaaagtca aagcattcat cgcagtttcc gattaatcag cgttaaatct
     4981 catatttctt ttcgtttttt tcatcgtgtc ggtaataagc ctttcgtttc gcgctcttta
     5041 aaatcgagct tgaaatcgaa ggagagatag agaatgaggc gaaacaatac tgcaaataat
     5101 ttttggcgcg tttttttagc gtttgccctc ggcgttttcg ttttcgcaaa ttacgccgaa
     5161 gcgcagacga aacgcccgca gcagaagccc gccgaacaaa taacaaaaca aatgaaagac
     5221 gaagtcacat acgagccgga tggcggtttg ctcaaggaaa tgtggtcgcg aatctcggtc
     5281 gtcgaatcga aaaaagattg gaagcaattt aagcgtcagc gtccgacgct caacaaatcg
     5341 ggcagtcgct cggcgtacat cgcgcagttt gcgattgaac ccggcggcga agcgttgatt
     5401 gacgtgatag tcgtctatga aaaatcgagc ggcaagtttt acgagcttcg cggctttgat
     5461 tttccgcgcc cgtttgtcga attgaaatgg acgagcgacg acgttttgca attcgagcaa
     5521 tgggtaaatc cgacgaacgg cgggcgatac gcgattaatg ttaaaagggg agaaatcgtt
     5581 tcggctggct atgtgagaag caattaaaga gggattttca gtttatgaaa aagcgtattt
     5641 tatcaatcgc aattttaatt ttaatttcgg caagcgccgg tttcgcgcag acaatcgagc
     5701 agcaggtgat aaggattcgc aatctttacg ccgccacaaa caagcggatt gacgaaggtt
     5761 taaaggacaa aatatccggc ttgcattacg ccgcgcggac aatcggcggc gagcgcgacg
     5821 ggcggcagtg ggcggcggtc ggcacgatga aaaaactcga cgaattctgg ttcgacggcg
     5881 agccggactt taacgaggaa gaaaatcccg acgcgcgaaa gatgattcgt aaaatcatct
     5941 cgacttacgc gggcgcggct gatttgcgaa cgcggtcgga atatcttttc gatgcggaga
     6001 cgggcgaatg cgttttcgct ttcacgaccg aactcggagc cgacggcaaa acgaccgagc
     6061 ggcgtttata ttttcataaa ggtaaattga ttcgcatcgc gcgcggcggc aaaaacgtgg
     6121 acgcgaattt ttccgccgcc gacctgaatg cggcgcaaat cgaatcggac gcggcaaaac
     6181 gcctgaaaaa tcttttcgcg ctgatgatga ctgggtaaat caaaaaagga attcgggaaa
     6241 acaacgcatc gaattttaat cgttatcgaa aaccgcgccg gcgatgtgat ttgatttaat
     6301 tcgtttcgcc gataagaacg aaaccagccc agtaaaacgg gtgcgc
//
LOCUS       DASWNE010000500         5134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_442106, whole genome shotgun sequence.
ACCESSION   DASWNE010000500 DASWNE010000000
VERSION     DASWNE010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5134
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442106"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VGB68_09890"
     CDS             complement(<1..363)
                     /locus_tag="VGB68_09890"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0049585.1"
                     /translation="MFQAGQKFGSYVLIRKLAKGGFGEVWLAEKRSQFVTKKVAMKLP
                     HAGQFNFEAIRQEATLWEKASGHPNVLPLIDADIYDGQVVILSEYIEDGSLADMLRKH
                     GRLAVQQAVRIVVGVLNGL"
     gene            complement(408..1304)
                     /locus_tag="VGB68_09895"
     CDS             complement(408..1304)
                     /locus_tag="VGB68_09895"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY0049586.1"
                     /translation="MKLWLSKNSEISLREQLTRQVMLAIASGDLQAGERLPSVREIAL
                     RLKIHPNTASAAYRWLEENGWVESRVGSGVYARKVAELKISETARQIESELDRLILSF
                     LNDARRRGFENKRIKTRLNFWLQAKKPQKIVIVENDSDLSRILACEITALLAAPVSVV
                     DFAKAEKDGFAGNSLIVSLSDSAENFAAPAAFVKLKLNSVQDSMRGKEKPGESDLVGV
                     VSHWEKFLRWSQTMLVAVGIDEENLVVRNASEEDWKKGLDSCRFVITDSLTAKHLPVN
                     IDKRVFQLISPGSIAEIQNYLK"
     gene            complement(1372..2367)
                     /locus_tag="VGB68_09900"
     CDS             complement(1372..2367)
                     /locus_tag="VGB68_09900"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY0049587.1"
                     /translation="MKQGTVKTLRHESEILKNNPLNDKHVRDVIVYLPPGYEQSGEAF
                     PTVYCLTGFTGRGRMLLNDSAFTPNLAERMDNLIDEGKIQPMIVVMPDCFTRYGGSQY
                     INSAATGNYEDYLTREIVPFVDENFKTIRDKNSRAVMGKSSGGYGALIMAMRRADLFG
                     LACSTSGDCCFEYCYLPDFPKAFRAVKGSPQKIIEKFWNEEENKSKDDFAALNIIGMS
                     ACYSPNPLSATGFDLPFDLKTGEIRGEIWAKWLAHDPARLVEKSVENLKSLKLLYIDA
                     GSRDEFALDIGAKILCKKLDDSGVPHVYEEFDDGHFNISYRYNRSLEMISKSVDS"
     gene            2581..3207
                     /locus_tag="VGB68_09905"
     CDS             2581..3207
                     /locus_tag="VGB68_09905"
                     /inference="COORDINATES: protein motif:HMM:NF022479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HEY0049588.1"
                     /translation="MASGDLINLNPNGLTSTIEFLTAMITPALLISATGSLVLSTSTR
                     LGRVIDRVRELENRLQELIVVEDKNEIPLYDKRLEVIVGLLDKVTSRSRILQRAMVAF
                     YYGLGFFILTSVTIAIAGILSIYRWLPIPVGVVGIMFLFYGSLLMLREARMATMTINA
                     EMDFTWELARTVAPEGVLSKYKRNVFKQGRLRAKIHETLSNRRTEDES"
     gene            3224..4159
                     /locus_tag="VGB68_09910"
     CDS             3224..4159
                     /locus_tag="VGB68_09910"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0049589.1"
                     /translation="MESCTVCPKDCGNNRLNDEIAACYSGRLPVVSSYTAHFGEEPVL
                     SGTRGAGNIFFGNCNLRCVYCQNYQISQTWKQQRKNEITHERLAEMMLELQAKGCHNI
                     GFVSPTHFAPQMARAIFLAAENGLRLPIVYNTNAYDSVEVLRLLDGVVDVYLPDLKYA
                     DSAAGFQYSKVRDYARFSRAAIKEMFRQTGDALVYDENGLLKRGLVIRLLVLPNDLAE
                     IRESLRWIRDELSPKTAVSLMAQYYATNKAATDERYILLSRRISEREWLAAVEALEEF
                     EMEEGFIQEYESASHYYRPDFSDAEKPFKDIRDFQ"
     gene            4190..5086
                     /locus_tag="VGB68_09915"
     CDS             4190..5086
                     /locus_tag="VGB68_09915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEY0049590.1"
                     /translation="MKPFLKWAGGKTQLIAEIQRNLPSLVLSGRFTYIEPFVGSGAVL
                     FWMLNNFPNLEKAVINDINSDLINTYEIIRSSPKELVSLLRDFQSEFHGLQNEEERKK
                     EYYYEKRDLYNTRSTDETTQAALFIFLNRTCFNGLYRVNKSNLFNVPMGSYKLPRICD
                     EENISAVSDALQNVEILNDDFEKTLSHANENTFFYLDPPYKPLSATSSFNSYAENEFD
                     DDEQIRLRDFCMKLDAQNSKWMLSNSDVGDNDFFDSLFKAFVIKRVDAKRIINSKAHK
                     RGNLSELLITNYKTQEAFLFSV"
BASE COUNT         1300 a         1232 c         1257 g         1345 t
ORIGIN      
        1 caagccgttg agcacgccga cgacgatgcg gacggcttgc tggacggcaa gcctgccgtg
       61 ttttctcaac atatcggcga gcgagccgtc ttcgatatac tcgctcagga tgacgacctg
      121 accatcgtaa atgtccgcgt caatcaatgg cagaacgttc ggatgcccgc tcgctttttc
      181 ccagagcgtc gcttcctggc gaatggcttc aaaattaaat tgaccggcgt gcggcagctt
      241 catcgcgact tttttggtca cgaattgcga gcgtttttcc gccagccaga cttcgccgaa
      301 accgcctttg gcgagctttc tgattaatac gtagcttccg aatttttgcc ctgcttgaaa
      361 cattgattta tttgattata agcaaaacgg aacggtttta tgtagattta tttaagataa
      421 ttttgtattt cggcgatcga gccgggcgaa atcaactgaa aaacgcgttt gtcgatatta
      481 accggcagat gtttcgccgt caggctgtcg gtaatcacaa accggcacga atccagacct
      541 tttttccaat cttcttcgct cgcgttgcgc acgaccagat tttcttcgtc gatgccgacg
      601 gcgacgagca tcgtctgcga ccagcgcaga aatttttccc agtgcgagac gacgccgacc
      661 aaatcgcttt cgccgggttt ttccttgccg cgcatcgaat cctgcacgga atttaatttc
      721 agcttgacga aagccgccgg cgcggcaaaa ttttccgccg aatccgaaag cgaaacgatt
      781 aaggaatttc cggcgaaacc gtctttttcg gctttggcaa aatcgacgac ggaaaccggc
      841 gcggcgagca gcgccgtgat ttcgcaggcg agaatgcggc ttaaatccga gtcgttttcg
      901 acaatcacga ttttctgcgg ctttttggct tgcagccaga aattcagacg cgtttttatt
      961 cgcttgtttt caaaaccgcg tcgtcgcgca tcgtttaaaa acgataaaat caaccggtcg
     1021 agctcgcttt caatctgccg cgccgtttcg cttattttca attccgccac ttttcgcgcg
     1081 taaacgccgc tgccgacgcg cgattcgacc cagccgtttt cttccagcca gcgatacgcc
     1141 gcgctcgccg tgttcgggtg aatcttcagc cgcagagcga tttcgcgaac gctcggaagc
     1201 cgctcgcccg cctgtaaatc gccgctcgca atcgccagca ttacctgccg cgtcaactgc
     1261 tcgcgcagag agatttcgct gtttttggaa agccagagtt tcataataac ggataactga
     1321 tagttgataa ctgataatga aagtaaggaa caaactctca actatcagtt atcaactatc
     1381 aactgatttt gaaatcattt cgagcgagcg attatagcga tacgagatat taaaatgccc
     1441 gtcgtcgaat tcttcgtaaa cgtgcggaac gccggaatcg tccagctttt tgcacaagat
     1501 tttcgcgccg atgtcgagcg caaattcgtc gcgcgagccc gcatcgatat acagcaattt
     1561 taagcttttt aagttttcca cagacttttc cacaaggcgc gccgggtcgt gcgccagcca
     1621 tttcgcccag atttccccgc gtatctcgcc cgtttttaaa tcaaacggca aatcgaagcc
     1681 cgtcgccgaa agcgggttcg gcgaataaca cgcgctcatt ccgataatat tcaatgcggc
     1741 aaaatcgtcc ttgcttttat tttcttcctc gttccagaat ttttcgatga ttttctgcgg
     1801 gctgcctttg acggcgcgaa acgctttcgg aaaatccggc agataacaat attcgaaaca
     1861 gcagtcgccg ctggtcgagc aagccagccc gaagaggtcg gcgcggcgca tcgccataat
     1921 cagagcgccg tagccgcccg aagatttgcc catcacggcg cgtgaatttt tatcgcggat
     1981 ggttttgaaa ttttcgtcga cgaacggcac gatttcccgc gtcaagtaat cttcgtaatt
     2041 tccggtcgcc gccgagttga tatactgcga gccgccgtag cgggtaaaac aatcgggcat
     2101 cacgacaatc atcggctgga ttttgccttc atcgatgaga ttgtccattc tttcggctaa
     2161 attcggcgtg aaagcgctgt cgtttaaaag catccgcccg cgtccggtaa aacccgtcag
     2221 gcagtaaacg gtcgggaaag cttcgccgct ttgttcgtat ccgggcggaa gatagacgat
     2281 tacgtcgcgg acgtgtttat cgttgagcgg gttatttttt aaaatctcgc tttcgtggcg
     2341 cagagtcttg acggttcctt gcttcataaa tttaattttt aacttaaaga cgcgaaggtg
     2401 caagcaagcg aagaaagaaa tttatccgga ggtgacgcgg agaggagaag aaattcacag
     2461 ggataacagg atgaacaagg ataagaaaga tattttttta aatccctgtc tatcctgtca
     2521 tccctgtgaa tttcttcctt tgctcttcgc ctctttgcgc ctttaagtta aactctcttt
     2581 atggcatccg gcgatttaat caatctgaac ccgaacggtt tgacttcgac catcgaattt
     2641 cttacggcga tgattacgcc cgccttgctt atttccgcga ccggctcgct ggtgctttcg
     2701 acctcgacgc ggctggggcg cgtcattgac cgcgtgcgcg agctggaaaa ccggcttcag
     2761 gaactgattg tcgtcgagga taaaaacgag attccgcttt acgataagcg gctcgaagta
     2821 atcgtcgggc tgctggacaa ggtgacgagc cgctcgcgga ttttgcagcg cgcgatggtc
     2881 gctttttact acggtctggg attttttatt ttgaccagcg tgacgattgc gattgccgga
     2941 attttgagta tttatcgatg gctgccgatt ccggtcggcg tcgtcggtat tatgtttctt
     3001 ttttacggaa gccttctgat gctgcgcgag gcgcgaatgg cgacgatgac gattaacgcc
     3061 gagatggatt ttacgtggga gctggcgcgc acggtcgcgc ccgaaggcgt gctcagcaaa
     3121 tacaaacgca atgtttttaa acagggaaga ctcagagcca aaattcacga aactttatcg
     3181 aacaggcgaa ctgaagacga gagttgaaga actggaaaaa ctcttggaat cgtgcaccgt
     3241 ctgtccgaaa gactgcggca acaatcgcct gaatgacgaa atcgccgcct gttattcggg
     3301 acgcctgccg gtcgtctcca gctacacggc gcatttcggc gaagaacccg tcttgtcggg
     3361 cacgcgcggc gcgggcaata tttttttcgg caactgcaat ctgcgctgcg tttactgcca
     3421 gaattatcaa atctcgcaga cgtggaaaca gcagcgcaaa aacgaaatca cgcacgagcg
     3481 gctggcggaa atgatgctgg agctacaggc gaaaggctgt cataacatcg gtttcgtcag
     3541 cccgacgcat tttgcgccgc aaatggcaag ggcgattttt ctggcggcgg aaaacgggtt
     3601 gcgtctgccg attgtttaca acacgaacgc ttacgattcg gtcgaagttt tgcggcttct
     3661 cgacggcgtc gtcgacgtct atctgccgga tttgaaatac gccgacagcg ccgccggttt
     3721 tcaatattca aaggtgcgcg attacgcgcg attttcgcgc gcggcgataa aagagatgtt
     3781 tcgtcagacg ggcgacgcgc tcgtttacga cgaaaacggt ttgttaaagc gcgggctggt
     3841 cattcgcctt ctggttttgc cgaacgatct ggcggaaatc cgcgaaagct tgcgctggat
     3901 tcgcgacgag ctttcgccga aaacggcggt ctcgttaatg gcgcagtatt acgcgacgaa
     3961 caaagccgcg accgacgaac gctatatttt attatcgcgc cgcatctccg agcgcgaatg
     4021 gcttgcggcg gtcgaagccc tggaagaatt cgagatggaa gaaggcttta tacaggaata
     4081 cgaatcggca tcgcactatt accgcccgga tttttcggat gcggaaaaac cttttaaaga
     4141 cattcgggat tttcagtaat tatctaaaca caataagctt aaaagcaaaa tgaaaccttt
     4201 tctaaaatgg gcgggcggaa aaacccaatt aattgcggaa atccaacgaa atcttccgtc
     4261 attagtttta tccggcagat ttacttatat tgagccgttc gtcggaagcg gcgcagtttt
     4321 gttttggatg ctgaataatt ttccgaattt agaaaaagct gtaatcaacg atattaattc
     4381 cgatttgatt aacacttacg aaataattcg ttcaagcccg aaagaattag tttcgctctt
     4441 aagagatttt caaagcgaat ttcacgggct gcaaaacgag gaagaaagaa agaaggaata
     4501 ttattacgaa aaaagagatt tgtataatac ccgaagcacg gatgaaacca cgcaagcggc
     4561 tttgtttatt ttcctgaatc gaacttgctt taacgggttg tatcgcgtca acaaaagcaa
     4621 tttattcaac gtgccgatgg gaagctataa actgccgaga atttgcgacg aagaaaatat
     4681 tagtgctgtc agcgatgcgc ttcaaaatgt ggaaattctt aacgacgatt ttgaaaaaac
     4741 gctgtctcac gcaaacgaaa acactttttt ttatctcgat ccgccatata aacctttgag
     4801 cgcaacttca agttttaatt cctacgctga aaatgaattt gacgacgatg agcaaattag
     4861 attaagggat ttttgtatga aacttgacgc tcaaaacagc aaatggatgt taagcaattc
     4921 ggatgttggt gataatgatt tttttgattc tctgtttaag gcttttgtta taaagcgagt
     4981 agatgcaaaa agaataatta attctaaagc tcataaaaga ggtaatttga gtgaattgct
     5041 gattaccaat tataaaacac aagaagcatt tttattttcc gtttaatcat ctgaaacccg
     5101 gcttagttgc ctgcattact tgtatgtaat caac
//
LOCUS       DASWNE010000501         3472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_582263, whole genome shotgun sequence.
ACCESSION   DASWNE010000501 DASWNE010000000
VERSION     DASWNE010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582263"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(299..1681)
                     /locus_tag="VGB68_09920"
     CDS             complement(299..1681)
                     /locus_tag="VGB68_09920"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 47 protein"
                     /protein_id="HEY0049591.1"
                     /translation="MKAKTIVVVLLFAILFSAFAPVSAQPKSVKNPKVDKARLAREVK
                     AEFLHAWNGYKQYAWGHDDLKPLSKGYKDWYAEPLLMSPVDALDTMIVMGFEREANKT
                     REYIAANLSFDKDIYVKNFEITIRLLGGLLSSYQLTKDKRLLNLADDLGRRLLPAFDS
                     PTGIPYMFVNLKTGKVRNAVTNPAEAGTLLVEFGTLSKLTGKPVYYEKAKKALVETYK
                     RRSKINLVGQRINVETGEWTNTNSSISAEIDSYYEYLLKCWLLFGDEDCRKMWQEHIV
                     AINKYLADETENGLWYGHADMNTGKRKATVYGALDAFFPAVLALSGDVERAKRLQDSS
                     YKMWILHGIEPEEFNYKTMKVEGATYYLRPEIVESAYYLHQFTGDAKYLQMGERFFND
                     FRKYCRTEAGYATLKNVETKEKMDVMESFVFAETFKYYYLLFTSPKEFRLKNVVFNTE
                     AHPIKKTWKN"
     gene            complement(1803..2678)
                     /locus_tag="VGB68_09925"
     CDS             complement(1803..2678)
                     /locus_tag="VGB68_09925"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 18 protein"
                     /protein_id="HEY0049592.1"
                     /translation="MTSSNSTVNAWIFLNEDEPSATNYDSPNSCFQALIQNNVYQAVD
                     MLFICFAVTVATGASTIPSGDGSSYTLQMGASSHPGGLTNQDYMNYLLRDARANNPNI
                     KFMMTLLWGDPDTLAKIFSNSNYSDEQNAENFAANLLAYLEYYDLDGFDLDWESPLSD
                     GTSENQMTLLLSAIGAQFQKQTGKKYYLTLSPATSTNLNGAAVNANVDFLNLQLYSGF
                     TYPGEFQSLGIDTGLFAYGAKFESNYQTAQAAYEDNRQNYGYGIFTNWRLNSENFEFE
                     QEQQVELHKLVFPND"
BASE COUNT          904 a          852 c          777 g          939 t
ORIGIN      
        1 cctgagccgc tcggcgctcg aatttgcaaa ggaaaatttc agcatcgaag ctcacctgcg
       61 caagctgaaa aagctttacg attcgatttt aaaataaaag aagaattagc gacagaggac
      121 acagagaaca cagagaaaga aaattgaaac cacagatgaa cgcagataaa cgcaaatttt
      181 aacagaatag acaggattat caggataatt gaaaactaag aacattaagc tttttttatc
      241 ctgtaaatcc tgcctatcct gtttgaatta ccctctgtgt tctctgtgtc ctccgtcgct
      301 aattcttcca cgtcttctta atcgggtgag cttcggtatt gaaaaccacg tttttcaagc
      361 ggaactcttt cggcgaggtg aaaagcagat agtaatattt gaaagtctcg gcgaaaacaa
      421 agctttccat cacgtccatt ttttcttttg tctcgacgtt tttcaaagtc gcgtaaccgg
      481 cttcggtgcg gcagtatttc cgaaaatcgt tgaaaaaccg ctcgcccatt tgcaggtatt
      541 tcgcgtcacc ggtgaattga tgcaggtaat aagccgattc gactatttcg ggacgcaggt
      601 aatacgtcgc gccttcgact ttcatcgttt tgtaattaaa ttcttcgggt tcgatgccgt
      661 gcagaatcca cattttatag ctcgaatcct gaagccgttt tgcccgctcc acgtcgcccg
      721 acagagccag aaccgccgga aaaaacgcgt cgagcgcgcc gtaaacggtc gccttgcgtt
      781 tccctgtgtt catatcggcg tgcccgtacc aaagcccgtt ttcggtttcg tccgccagat
      841 atttattgat ggcaacgatg tgttcctgcc acattttgcg gcaatcctcg tcgccgaaca
      901 gaagccagca tttcaaaaga tactcgtaat aagaatctat ctcggcgctg atgctggagt
      961 tcgtgttcgt ccattcgccc gtctcgacgt tgatgcgctg accgaccaga ttgattttcg
     1021 agcgtcgctt ataggtttcg accagcgctt ttttcgcttt ctcgtagtaa acgggttttc
     1081 cggttaactt gctcaaggtt ccgaactcga ccagcaaagt tcccgcctcc gccggattcg
     1141 tcacggcatt tctgactttg cccgttttca gattgacgaa catatacgga attccggtcg
     1201 gcgaatcgaa cgcgggcaaa agccgcctgc ccaaatcgtc ggcgagattt aaaagccgct
     1261 tgtcttttgt caattgatag ctcgaaagca gaccgccgag aagccgaatc gtgatttcaa
     1321 aatttttaac gtaaatgtcc ttgtcgaatg aaagattggc ggcgatatac tcgcgcgttt
     1381 tgttcgcttc cctctcaaaa cccatcacaa tcatcgtgtc gagcgcgtcg accggcgaca
     1441 tcagaagcgg ctcggcatac caatccttgt agcctttgct cagcggcttt aaatcgtcgt
     1501 gtccccaggc gtattgcttg tagccgttcc acgcgtgcag aaactcggct ttgacttctc
     1561 gcgccagcct cgctttatcg actttcggat ttttcacgct tttcggctgc gcgctgaccg
     1621 gcgcgaaggc ggaaaataaa atggcgaaca ataaaacaac aacgattgtt ttagctttca
     1681 taccggcgag aatatcagat ttcaacggcg aattaaaaat tgcgagcgaa cgattttcga
     1741 cctttgacct ttgaaaaaca accgaaaatc aaaggtcaaa ggtcatttcc tttaattcgc
     1801 cgttaatcgt tcgggaaaac cagtttatga agctcgacct gctgctcttg ttcaaactcg
     1861 aagttttccg aattcaagcg ccagttagtg aaaatgccgt agccgtaatt ttgccggttg
     1921 tcttcgtaag cggcttgcgc ggtttgataa ttcgattcga actttgcccc gtaggcgaac
     1981 aagccggtgt cgatgcccag actttgaaac tcgcccggat aagtaaatcc cgaatacaac
     2041 tgtagattca aaaaatcgac attggcgttg accgccgcgc cgttcagatt cgtgctggtc
     2101 gcgggcgaga gcgtcaggta atatttttta cccgtctgct tttgaaactg cgcgccgatg
     2161 gcgctcagca gcagcgtcat ctggttttcg ctcgtcccgt cggaaagcgg cgattcccag
     2221 tcgaggtcaa agccgtccag atcataatat tccagataag cgagcaggtt ggcggcgaaa
     2281 ttttcggcgt tctgctcgtc cgaataattg gaattggaaa atatcttcgc cagcgtgtcc
     2341 gggtcgcccc agagcagcgt catcataaat ttgatgttcg gattgttggc gcgcgcgtcg
     2401 cgcagcaggt aattcatata atcctgattg gtcagcccgc ccggatgcga cgacgcgccc
     2461 atttgcagcg tataggaaga accgtcgccg ctcggaatcg tgctcgcgcc ggtcgccacg
     2521 gtcacggcga aacagatgaa cagcatatcg accgcttgat agacgttatt ctgaatcagc
     2581 gcctgaaaac aactgttcgg actgtcgtag ttagttgccg aaggctcgtc ttcgttcaaa
     2641 aaaatccagg cgttgaccgt tgaattgctc gatgtcataa attttccttt ttatttaaga
     2701 ttcaggcgct tgcgtcctga aaatcctgat tccggaaacc ggatgcgaaa tgagaaaagc
     2761 gcatataaat cacttgcttt cattcatttt cctttgagat gtataagatt aaatttcccc
     2821 gcggcatttc aaggaagaat aaacggaagg agttttctct gaatcgagcg cggtagattt
     2881 gcctgaatac cgacgtgaaa agctttgtgt aattcgactg cattcacgtg ggcgaaaggt
     2941 tcgcgcagta gaacaaagat acatttatta aatcttgaac cgcaataaat atataatcct
     3001 aaattcaaaa taaagcaata tttttttgcg gcgtgaattt aacgttttct tatttacaaa
     3061 ctcaatccgg agacttacaa atccaatctg caagcctgca aacgtctttt gttaattaac
     3121 aaacccgatt cggaagctta caaacccgat ttgttaatct gcaaacgcaa tctgtaagct
     3181 tccgaatcca ttttgtaagc ttacaaacgc catttgtaag tctccaaacg tcatttgtaa
     3241 gctcgaaaac gatgatttcg catgcgttta atacattcta cttcgcccat ccaaaaacaa
     3301 aacgccgccc gaaaagcatc gagcggtgtt ggaaagtatt tatttaatgc ccggcatcat
     3361 tcatttacga tataggcata cagcgtgcaa attttctaat ccttccggct ttgcccgacg
     3421 aaagccttat gcccgacgaa acgcgctctc gccgaaaata acttcgggct at
//
LOCUS       DASWNE010000502         5918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_86365, whole genome shotgun sequence.
ACCESSION   DASWNE010000502 DASWNE010000000
VERSION     DASWNE010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5918
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86365"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1272
                     /locus_tag="VGB68_09930"
     CDS             <1..1272
                     /locus_tag="VGB68_09930"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0049593.1"
                     /translation="LFASEKDGFNHLYLATLEKRKPEPNQSGEIRQENQADAGFTTNV
                     SIKQLTKGNFEIDWAKWSAKGETIVFSSTEENSAERQFYNFDARTNEKTQVHSFEKGM
                     KTNPQIDKVNGNPTLIYEFSQWNQPTELFTTKACANCRGLAVPKQITKSTPESFKKIN
                     WSAPKFVDIPTRDGKNVKSKIYLPANFSQKQKYPMVIFVHGAGYLQNTINGWNNYYRE
                     FMFHTILTQKGYVVLDIDYRGSAGYGRDWRTDVYDFLGGKDYDDHLDAIDFAVRNYPV
                     NPQKIGVYGGSYGGFMAEMLVMRAPERIAAAAALRPVADWKNYFASSPVYTAERLGFP
                     DKNPEAYRRSSPIAYADKLQKPLLILHGMVDDNVHAQDSIQLIEKLIRLEKTPYFEAM
                     MYPSENHGFQRPTSWADEYERILMFFEKHLK"
     gene            1403..2341
                     /locus_tag="VGB68_09935"
     CDS             1403..2341
                     /locus_tag="VGB68_09935"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEY0049594.1"
                     /translation="MRILITGGAGFIGSHLCERLLAEGNEVIALDNFFTGRKENVAHL
                     LDNKNFELVRHDVTEPILLEVDQIYNMACPASPVHYQYNPVKTVKTSVMGAINMLGLA
                     KRVNARILQASTSEVYGDPLIHPQTEDYYGNVNCIGLRSCYDEGKRVAETLMMDYHRQ
                     NQVDTRIVRIFNTYGPRTLENDGRVISNFIVQALRGEELTIYGSGEQTRSFCYVDDLV
                     EGIIRLMNTEEESLHLPVNIGNPGEFTMNELANLVGETIGREIKIKHMPLPQDDPKQR
                     KPNIERAKNLLKWQPTIPLAEGLKKTVAYFAERIKS"
     gene            2367..2621
                     /locus_tag="VGB68_09940"
     CDS             2367..2621
                     /locus_tag="VGB68_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049595.1"
                     /translation="MSLENRMTAVEKAVLTLEKLIISHDERLEDYYKALEESRRDFEF
                     KMNALIDSQIRTEAEIRGLKETSNSTLERLDRLERKNGDK"
     gene            2639..3625
                     /locus_tag="VGB68_09945"
     CDS             2639..3625
                     /locus_tag="VGB68_09945"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEY0049596.1"
                     /translation="MKILITGGAGFVGSHLADKLHKEGNEITVIDNLSTGRYSNVAHL
                     EGEDGFRLIIDTVLNARLMEELIRESDRVFHMASAVGVRLIMEQPVKTIETIFHGTDI
                     VLGFCARYRTRVLIPSTSEVYGKGTSVPFREEDDILKGSTSKHRWAYACAKELDEFLA
                     LAHWKESRLPVSVIRLFNTVGPRQTGQYGMVVPRFVQSAIKGESITVHGDGTQSRCFG
                     HVLDVVEGLTKAIETPACFGQVMNLGNDEEISIRELAEKAIQITGSASEIQYIPYDEV
                     YGEGFEDMQRRVPSLEKAERLIGYQPTRTLNDIINDVAEEFRQSLKSETANA"
     gene            3720..4349
                     /locus_tag="VGB68_09950"
     CDS             3720..4349
                     /locus_tag="VGB68_09950"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY0049597.1"
                     /translation="MSMKFLKQSVLLALLFVLASVVAFGQSTSGVKGKVRTARGGGIA
                     EATVTARQKGQDIKTVTTDSKGEFVLDGLESGSYNLVFTKNGYGAGTLYNIEVKKNKI
                     TDLPDRLILTTDQGTQVIVRGSVFNQDGVSLYGVKVEIERIAEGGARKKVGSGYTSEA
                     GEFTFRFPEGTAKFRVTASAKGVSASKEVTVDNAAIYRLAITLNLAKEN"
     gene            complement(4501..4935)
                     /locus_tag="VGB68_09955"
     CDS             complement(4501..4935)
                     /locus_tag="VGB68_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049598.1"
                     /translation="MTNGNLQLIYTLLCDDVRLETGNKISLMGVFQNIMVEKLPVSLI
                     KFAVVNHWRGDGNHSAEVRILSPDKSNLVVTSQPTHIELVPGGFTDNVSFFVNVVFPN
                     AGTYWVQTLANSVLLEEFPLIVTDARAVSGVQTAEEISETVN"
     gene            complement(5048..>5918)
                     /locus_tag="VGB68_09960"
     CDS             complement(5048..>5918)
                     /locus_tag="VGB68_09960"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY0049599.1"
                     /translation="DPAAPVPKLDRREFLVSSIFAAGVFAAAVLPIQGQTKITTDTKG
                     LITGEVKIPVAGGMMPAYRAMPDKKGKNFPVVLVIHEIFGVHEWIQDVSRRFAKLGFM
                     AIAPALYARQGDVSTISTAQELNRAIFSKIPDTQSMADLDATVNWAKNNSGNTKKLSI
                     TGFCWGGRITWLYAAHNKNVDAGAAWYGRVVPTPNSPKNEAQPTTPIDYAKDLKVPVL
                     GLYGGLDKGIPVDGVQLMQDALKKGKSKSEIIVYPNADHGFLADYRPSYNKEAAADAW
                     AKMLDWFKKNGAA"
BASE COUNT         1551 a         1446 c         1541 g         1380 t
ORIGIN      
        1 cttttcgctt cggaaaaaga cggttttaat catctctatc tggcgacgct ggaaaaaaga
       61 aagcccgaac caaaccagtc gggcgagatt cggcaggaaa atcaggcgga cgcgggattt
      121 acgacaaatg tttcgataaa acaattgacg aaaggaaatt tcgagattga ttgggcgaaa
      181 tggtcggcga agggcgaaac gattgttttc agctcgaccg aagaaaattc cgccgagcgg
      241 cagttttaca atttcgacgc gcgaacaaac gagaaaactc aggttcattc gtttgaaaaa
      301 ggaatgaaaa cgaatccgca gattgataag gtaaacggca atccgacttt gatttacgaa
      361 ttttcgcagt ggaaccagcc gacggagctt tttacaacca aagcttgcgc caactgtcgg
      421 ggcttggcag taccgaaaca aattacaaaa tcgacgcccg aaagctttaa aaaaatcaat
      481 tggagcgcgc cgaaattcgt cgatattccg acgcgcgacg gcaaaaacgt gaaatcgaaa
      541 atttatctgc ctgccaattt cagtcaaaaa caaaaatatc cgatggtgat tttcgttcac
      601 ggcgcaggct acctgcaaaa cacaatcaac ggctggaaca attattaccg cgagtttatg
      661 tttcacacga ttttaacgca gaaaggctac gtcgtgctgg acatcgacta tcgcggctcg
      721 gctggctacg ggcgcgattg gcgcacggac gtttacgatt ttctgggcgg caaagattac
      781 gacgaccatc tggacgcgat tgatttcgcc gtcagaaatt acccggtcaa tccgcagaaa
      841 atcggcgttt acggcggcag ctacggcgga tttatggcgg aaatgctcgt aatgcgcgcg
      901 cccgagcgca tcgccgcggc ggcggctttg cgtccggtcg ccgactggaa aaattatttt
      961 gcctcgtcgc cggtttacac agccgagcgt ttgggctttc ccgacaaaaa cccggaagcg
     1021 tatcgacgct cgtcgccgat tgcctacgcc gacaagctgc aaaaaccgct tttgattctg
     1081 cacgggatgg ttgacgacaa cgtccacgcg caggattcga ttcaattaat cgaaaaattg
     1141 attcggctgg agaaaacgcc ttatttcgaa gcgatgatgt atccgtcgga aaatcacggt
     1201 tttcagcgcc cgaccagttg ggcggacgaa tacgagcgga ttttgatgtt ttttgaaaag
     1261 catctaaaat aggaaggatt tagccacaga ggacacagag ttcacagaga aaataaagaa
     1321 aacaagtaaa tttctgagtt attcgcacat ttatggttaa atatctctct gtgttctctg
     1381 tgagctctgt ggcaaaaaat ttatgagaat tttaatcacc ggcggcgcgg gttttatcgg
     1441 ctcgcatctt tgcgaaagac ttctggcgga aggcaacgag gtcatcgcgc tcgacaattt
     1501 tttcacgggg cgcaaggaaa acgtcgcgca cctgctggac aataaaaatt tcgaactggt
     1561 tcgtcacgac gtgaccgagc cgattttgct cgaagtcgac cagatttaca atatggcgtg
     1621 tccggcgtcg cccgtgcatt atcagtacaa tccggtgaaa accgtcaaga cgagcgtgat
     1681 gggcgccata aatatgctcg gcttggcgaa acgcgtcaac gccagaatct tgcaggcttc
     1741 gacctcggaa gtttacggcg acccgctcat tcacccgcaa accgaagatt attacggcaa
     1801 cgtcaactgc atcgggctgc gctcgtgtta cgacgagggc aagcgcgtcg ccgaaacttt
     1861 gatgatggat tatcatcgcc agaaccaggt cgatacgcgc atcgtgcgca ttttcaacac
     1921 ttacggaccg cgaacgctcg aaaatgacgg gcgcgtcatc tcgaatttta tcgttcaggc
     1981 gctgcgcggc gaggagctga cgatttacgg cagcggcgag cagactcgct cgttttgcta
     2041 cgtcgatgat ttggtcgaag gcattatccg gctgatgaat acggaagaag aatcgcttca
     2101 tctgcccgtc aatatcggca atcccggcga gtttacgatg aacgagctgg cgaatctggt
     2161 cggcgaaaca atcggcaggg aaatcaaaat caaacatatg ccgctgccgc aggacgaccc
     2221 gaaacagcgc aagccgaaca tcgaacgcgc gaaaaatctg ttaaaatggc aaccgacgat
     2281 tcctctggca gaaggcttga agaaaacggt cgcgtatttt gcggaaagaa tcaaatcgta
     2341 ggaaaatgtt ttgacgggag caacatatga gcctcgaaaa tagaatgact gcggtcgaga
     2401 aagcggttct gactttggag aagttaatca tcagtcacga cgaacgactt gaggattatt
     2461 acaaggcgct ggaagaatcg cggcgagatt ttgagtttaa gatgaacgct ctgattgatt
     2521 cgcaaatcag aaccgaagct gaaatcagag gattgaaaga aacatcaaac agcactttgg
     2581 aaagactaga ccgtttggaa agaaaaaacg gcgataaata aatttaagaa gaacacgaat
     2641 gaaaatttta attacgggcg gcgcgggctt tgtcggttcg catctcgccg acaaactaca
     2701 taaagaaggc aatgaaatca cggttatcga caacctctcg accgggcgtt attcaaacgt
     2761 cgcgcatctg gaaggcgaag acggttttcg tttgattatc gacacggttt taaacgcgcg
     2821 tctgatggaa gaactgattc gcgaatcgga cagagttttt catatggcga gcgccgtcgg
     2881 cgtccgtttg attatggaac agccggttaa gaccatcgag accatttttc acggcacgga
     2941 catcgttctc ggcttttgcg cgcgttatcg cacgcgcgtt ttgattcctt cgacctccga
     3001 ggtttacggc aaaggcacga gcgttccctt tcgcgaagaa gacgatattt tgaaaggctc
     3061 gacctcgaaa catcgttggg cttacgcctg cgccaaggaa ctggacgaat ttctcgcgct
     3121 ggcgcactgg aaagaatcgc gcctgccggt ttcggtcata cgccttttta acacggtcgg
     3181 accgcgccag accgggcaat acgggatggt cgtgccgcgc tttgtccaat cggcgataaa
     3241 gggcgagtcg attacggttc acggcgacgg cacgcagtcg cgttgtttcg gtcacgttct
     3301 ggacgtggtc gaaggcttga cgaaagccat cgaaactccg gcgtgtttcg ggcaggtgat
     3361 gaatctgggc aacgacgagg aaatttccat cagggaactg gcggaaaaag ccatccagat
     3421 taccggaagc gcgagcgaga ttcaatatat tccctacgac gaagtttacg gcgaaggttt
     3481 tgaagatatg cagcggcgcg ttccgagcct cgaaaaagcc gagcgtttaa tcggttacca
     3541 gccaacccga acgctgaacg acatcatcaa tgacgtagcg gaagaatttc gccagtcttt
     3601 gaaatccgaa accgcgaacg cataaaagtt tgaagccgcg agccgcacgt gctaacacga
     3661 attattttct gttcgtgtta ttcgccggat tcgcggctaa attctttgcg gtaaatttta
     3721 tgagtatgaa gtttttgaaa caatccgttt tactcgcgtt attattcgtt ctggcatcag
     3781 tcgtcgcttt cgggcaaagc acgagcggcg tgaaaggaaa agtccgcacg gcgcgcggcg
     3841 gcggaatcgc cgaggcgacc gtcacggcga gacaaaaagg acaggacatc aagaccgtca
     3901 ctaccgattc gaaaggtgaa ttcgttttag acggtctgga aagcggctcg tacaatctcg
     3961 ttttcaccaa aaacggctat ggagccggaa cgctttacaa tatcgaagtc aaaaaaaata
     4021 aaatcaccga tcttcccgac cgattgattt tgacgaccga ccagggcacg caggtcattg
     4081 tcagaggaag cgttttcaat caggacggcg tcagtttata cggcgtaaaa gtcgagattg
     4141 aaagaatcgc cgaaggcggc gcaaggaaaa aagtcggctc gggatacacg agcgaagcgg
     4201 gcgaatttac ttttcgtttt ccggaaggaa cggcaaaatt tcgcgttacg gcttccgcca
     4261 aaggcgtttc ggcgagcaag gaagtcaccg tggacaacgc ggcgatttac cgtctggcga
     4321 ttactttgaa tttagcaaaa gaaaattagg tgcgcacaaa tcccaaaagg cacaaacacg
     4381 cacgaaagaa gaattatcct gttatccccg gtatttttct ttcgtgcgtg tttgtgcggc
     4441 ggcggaattt cttaattctt tgtccgtatt ttttcgtgct cggttgcggc taaatctttc
     4501 ttaattgacg gtttccgaaa tttcctccgc cgtctgcact cctgaaaccg ctctcgcatc
     4561 ggtcacaatc aacggaaatt cttccagcaa aaccgagttt gccagcgtct gcacccaata
     4621 cgttccggca ttcggaaaca cgacgttgac gaaaaagctg acgttatcgg taaatccgcc
     4681 cggcaccagc tcgatgtgcg tcggctgcga ggtgacgacc agattcgatt tatcgggcga
     4741 gagaatcctg acttccgccg agtgatttcc gtcgccgcgc cagtggttga cgaccgcaaa
     4801 ttttatcagc gagacgggca gcttctcgac cattatgttt tgaaacacgc ccatcagaga
     4861 gattttgttg cccgtctcca atcgcacgtc gtcgcacaaa agcgtgtata taagttgtag
     4921 atttccgttt gtcatttaat taattaacgt ccgaagacgc aaaaggcgca agttttgttt
     4981 tgacttagaa actatcaata ctcgataaat ttcaaacttt cgcccttcgc ccttcatctt
     5041 tcgcttttta agccgctccg tttttcttaa accagtcgag cattttcgcc caggcgtctg
     5101 cggcggcttc cttgttgtaa ctcgggcgat aatccgccag aaaaccgtgg tcggcatttg
     5161 gataaacaat gatttccgat ttcgattttc cctttttcag cgcgtcctgc atcagctgca
     5221 cgccgtcgac cggaatgcct ttatccaaac cgccgtaaag ccccaaaacc ggaactttta
     5281 aatctttggc gtaatcaatc ggcgtggtcg gctgcgcttc gtttttcggc gagttcggcg
     5341 tcgggacgac tctgccgtac caagccgcgc cggcatcgac gtttttgtta tgcgcggcat
     5401 aaagccaggt gatgcgaccg ccccagcaaa agccggtaat cgaaagcttt ttggtgttcc
     5461 cgctgttatt ctttgcccaa ttgacggtcg cgtccaaatc agccatcgac tgcgtgtccg
     5521 gtatttttga aaagatagcg cggttcagtt cctgcgccgt gctgatagtg gaaacgtcgc
     5581 cctgacgggc gtaaagcgcg ggcgcgattg ccataaaccc cagtttcgca aagcgccggg
     5641 aaacgtcctg aatccattcg tgaacgccga aaatctcgtg tatgaccagg acgaccggaa
     5701 agtttttgcc ttttttatcg ggcatcgcgc gataggcggg catcattccg ccggcaaccg
     5761 gaatttttac ttcgccggtg attaaacctt tagtgtcggt cgtgatttta gtctgcccct
     5821 gaatcggcag aacagccgcg gcaaaaacgc cggcggcgaa aatcgagctg accagaaatt
     5881 ctcgccggtc gagtttcggc acgggcgcag ccgggtcg
//
LOCUS       DASWNE010000503         3220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_613216, whole genome shotgun sequence.
ACCESSION   DASWNE010000503 DASWNE010000000
VERSION     DASWNE010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613216"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(352..636)
                     /locus_tag="VGB68_09965"
     CDS             complement(352..636)
                     /locus_tag="VGB68_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049600.1"
                     /translation="MKRKFCILVLMLASALIILPAESRAAVSGDSSNSASEYNYEPQI
                     RVQIGRNRRNRRWQRRANRQWRRSQMRRGRVVTQTYWRNGRRYTRVVRVY"
     gene            829..3210
                     /locus_tag="VGB68_09970"
     CDS             829..3210
                     /locus_tag="VGB68_09970"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEY0049601.1"
                     /translation="MFISMRNGLNRILMIVFCSLAASVCPAQTAPQNSALAGLKESVT
                     VRRDGRGIPYIEANNDADLYFAQGFVTAQDRLWQMDVYRRVARGELSEIFGGQVLEED
                     KRWRRFGFAKIVEDGLPSLSPELRAALESYSRGVNAYIATLDDKTLPVEFRILQYKPR
                     EWRTTDSLVIGKILADALSSTWHTDLLRASLQNLPKEKLSDLTNNVTPFDVVLFGKDL
                     AAERTEKTVKPKSQIMADENRFEKNSSFPSVFSAAEKESELRRSSLERAGFYAEELAA
                     SNNWVISGKRTLDGKPILANDPHLLSSAPGIWYLSHLAAPNVRVSGVTFPGVPGIVLG
                     HNEFIAWGATNVGPDVQDLYREQFDAQNRVKTPRGWENAKTRREEIKVRTNPLSPATE
                     TQFLDVFETRNGVVIAEEAGQKLALKWTALDPKNQEFEAFFVLNRARNWEDFKNALRK
                     YGGATQNFVYADTSGNIGWYAAGRIPIRRTGEGELPYDGATDAGDWTGYIPFEELPHL
                     YNPAEGFIVTANQRIVGTSYKYRQMSRDAAPPWRARRIFDLINANPKITMNDVRDIQY
                     DSFNIPLSNLAREVVKVSAIPEVKQLLGGWDGRMTADSKGAVVANEIRNCLANQIAGD
                     NMPVPFYVIRERIVDWAVAEKSARWLPKRFAGYSDLINACEKDSLGSLERRLGTDKEK
                     WVWGNVFQARFQHPLAIAPLIGGQFVIKPVGLNGSGQTPNVGSYVSMRHIASPGNWDA
                     TRQVIPLGQSGNPQSPHWKDQFEAWRTGSPQIFPFSKDAVQKAAKETITLQPK"
BASE COUNT          788 a          801 c          889 g          742 t
ORIGIN      
        1 acggcagcat agacagtaac ttctttttca gtcttgactt atccgcgagc gctttggtcg
       61 agcaggcgga cggcaaaatt ctggtcgccg gaagttttat gcacgtaaac ggcacgaggc
      121 gaaacggctt gatgcgtctg acgccgtaag taattaacgc aaaaggcgac gaaaattcaa
      181 aacgcaaaac gcaggaaccg ctaaaatttg aatcctgaat tctaaatcct aatattttgc
      241 attatgcgtt ttgaattttg ccttttttac gttttgcatt cgtcattttt ttcaaaagaa
      301 aaagcacggc gatttgccgc gctttcttca ttaaatttaa ttgtccgccg attaatacac
      361 acgcacgacg cgcgtgtagc ggcgaccgtt gcgccagtag gtctgcgtca caacccgacc
      421 gcgtctcatt tggctgcgtc gccattgacg attagctctg cgctgccacc gacgattgcg
      481 ccgatttctg ccgatttgaa cccggatttg cggttcgtaa ttatattcgg aagccgaatt
      541 ggagctgtct cccgaaaccg ccgcgcggct ctcggcaggt aaaatgatga gcgccgaggc
      601 gagcatcaac accagaatac aaaattttct tttcattaac ttctcccttt actcataaaa
      661 tttacgcctg taaaaaccgg cgcagacctt tatcgtagca attggcagat tgttaaagca
      721 agggatttaa aataaaaaaa agactgaaac gaaaagggaa atttcgttct gtttttcgtt
      781 tttcgcctgc tttggagtct cgcctttatc tatcggataa aattttgtat gttcatatcc
      841 atgagaaacg gtttgaatcg gattttgatg attgtctttt gttcgcttgc ggcgtcggtt
      901 tgtccggcgc agaccgcgcc gcagaattcg gctttggcgg gtttgaagga aagcgtcacc
      961 gtccggcgcg atggacgcgg gattccttat attgaggcaa acaacgacgc ggatttgtat
     1021 tttgcgcagg gattcgtgac ggcgcaggac aggttgtggc agatggacgt ttatcggcga
     1081 gtcgcccgcg gggaattgtc ggaaatcttc ggcggtcagg ttttggaaga agacaaacgc
     1141 tggcgtcgtt tcggtttcgc aaagattgtc gaggacggtt tgccgtcgct ttcgccggaa
     1201 ttgcgcgccg ctctggaaag ttattcgcgc ggcgtgaacg cttacatcgc gacgctggac
     1261 gataaaactt tgccggtcga gttcaggatt ctgcaataca agccgcgcga atggcgcacg
     1321 accgattcgc tggttatcgg aaaaattctg gctgacgcgc tcagctcgac gtggcatacg
     1381 gatttactgc gcgcttcact gcaaaattta ccgaaagaaa agctttcgga tttgacgaat
     1441 aacgtcacgc cgtttgacgt cgtgctgttc gggaaagatt tagccgccga gcgcacggag
     1501 aaaaccgtga aaccgaaatc gcagattatg gcggacgaaa accgatttga aaaaaattcc
     1561 tcatttccct ctgtattttc tgcggctgaa aaggaatccg aactccgtcg aagctcactg
     1621 gaaagagccg gtttttacgc cgaagagctg gcggcgagca ataattgggt gatttccggc
     1681 aagcggactc tcgacggaaa gccgattctg gcaaacgacc cgcatttgct gtcgtccgca
     1741 ccgggcatct ggtatctgtc gcatctcgcc gcgccgaacg tgcgcgtctc aggcgtgact
     1801 tttccgggcg tgccgggcat cgtgctcgga cacaacgaat ttatcgcctg gggcgcgacc
     1861 aacgtcggtc cggacgtaca ggatttatac cgcgagcaat tcgacgcgca gaatcgcgtg
     1921 aaaaccccgc gcggctggga gaacgcgaaa acgcgccgcg aagaaataaa agtgcgcacg
     1981 aatccgcttt cgcccgccac tgaaacacag tttttagatg tttttgaaac gcgcaacggc
     2041 gtcgtcatcg ccgaagaagc cggacagaaa ctggctttga aatggacggc gctcgacccg
     2101 aaaaatcagg agttcgaagc gtttttcgtt cttaaccgcg cccgcaactg ggaagatttc
     2161 aaaaatgccc tgcgaaaata cggcggcgcg acgcagaatt ttgtttacgc ggatacatcg
     2221 ggaaacatcg gctggtatgc ggcgggcaga atcccgattc ggcgcacggg cgaaggcgaa
     2281 ttgccgtatg acggcgcgac cgatgcgggc gattggacgg gctacattcc gtttgaagaa
     2341 ctgccgcatc tttataatcc ggcggaagga tttatcgtca cggcaaacca gcgcatcgtc
     2401 ggcacgagct ataaataccg tcagatgtcg cgcgacgccg cgccgccctg gcgcgcgcgc
     2461 cggattttcg atttgataaa cgccaatccg aaaataacta tgaacgacgt ccgcgacatc
     2521 caatacgatt cgtttaatat tcccttgtcg aaccttgcgc gcgaagtcgt caaggtcagc
     2581 gcgattcctg aagtgaaaca gcttttaggc ggctgggacg ggcgaatgac ggcggattcg
     2641 aaaggcgcgg tcgtggcaaa cgaaattcgc aattgtctgg caaaccagat tgccggagat
     2701 aatatgcccg tgccgttcta cgtcataaga gagagaattg tggattgggc ggtcgcggaa
     2761 aaatccgccc gctggctgcc gaaaaggttt gccggttatt ccgacttaat aaatgcctgc
     2821 gaaaaagatt ctctggggag cttggagaga agattgggca cggataaaga aaaatgggtt
     2881 tggggcaatg tttttcaggc tcgtttccag catccgctgg cgattgcgcc cttaatcggc
     2941 gggcagtttg tgattaaacc ggtcgggctt aacggaagcg ggcagacgcc gaacgtcggc
     3001 tcctacgtct cgatgcgcca tatcgccagc cccggcaatt gggacgcgac gcgtcaggtc
     3061 atcccgctcg gacaaagcgg caacccgcaa agtccgcact ggaaagacca gttcgaagcg
     3121 tggcgcacgg gcagcccgca gattttcccg ttttcaaaag acgctgtgca gaaagcggcg
     3181 aaggaaacta tcactctgca gcctaaatga caaaagggta
//
LOCUS       DASWNE010000504         4644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_652693, whole genome shotgun sequence.
ACCESSION   DASWNE010000504 DASWNE010000000
VERSION     DASWNE010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652693"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /locus_tag="VGB68_09975"
     CDS             complement(<1..1173)
                     /locus_tag="VGB68_09975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0049602.1"
                     /translation="MKIVRIIARLNVGGPARHVVWLTKALQNAEFQSVLMTGTVPEGE
                     EDMSYFAAAHEVAPVFIPEMSRELSAKDIISLWKVYRFLQKENPDIVHTHTAKAGTIG
                     RVAGFFYRWLTLRTLIGQPRAVRFVHTYHGHVFHSYYGRRKTSFFLFIEKALARLATD
                     KIITISRQQFEEIHHRFGVGKTKQFAVVPLGIDLSSFAEWQAKRAPLREEIGARDDEI
                     LVGLVGRLTEIKNHSLFLRAAQSYKENFDFPPLKFVIIGDGHLREKLEKEAEKYNIKD
                     IVTFLGNRSDADVFYPALDIVALTSLNEGTPLSLIEAMANARPCISTAVGGVIDLLGA
                     ERQNETDGFTVCERGVRTASGDAEGFLNGLIYLAKNEKLRKELGARGLDFVFASYDK"
     gene            complement(1388..1894)
                     /locus_tag="VGB68_09980"
     CDS             complement(1388..1894)
                     /locus_tag="VGB68_09980"
                     /inference="COORDINATES: protein motif:HMM:NF013103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEY0049603.1"
                     /translation="MSQQTAKENTFTKGRIEDVVVYQLKKFVDERGWLAELFRHDEIS
                     REFYPAMAYISVTEPNMQRGPHEHVEQADLFCFIGAGNFKLRLWDNRADSPTFRYVMT
                     LFVGADSPQAVIVPKGVAHAYKNISQTEKGVVINCPNRLYMGEGKKEAIDEIRHEDDP
                     ETIFTIDD"
     gene            complement(1919..2767)
                     /locus_tag="VGB68_09985"
     CDS             complement(1919..2767)
                     /locus_tag="VGB68_09985"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEY0049604.1"
                     /translation="MSNETILNITPDRLSELQSAWDAAKPFRYVVIDDFLTADFAEEI
                     LAAYPTPTGGAWEDTPYIHQLKKLTLNKNFPAPIGDFFRMTETEEFRNLVSSICRIPD
                     LIADPTLSGGGLHQIMNGGYLDVHVDYNFHPRTKEHRRLNLLLYLNKDWKREYEGYLE
                     FWDMQEKRQIENIAPVFNRAVIFETNEVSYHGHPRKLNLPPDVTRKSLAVYYYTKERE
                     EVETAMEHNTIFKQTTGFSGYVKTSLAAVLTLTERASSAAKKDLLETLSQRLYRKIKG
                     LPRENK"
     gene            complement(2805..3533)
                     /locus_tag="VGB68_09990"
     CDS             complement(2805..3533)
                     /locus_tag="VGB68_09990"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY0049605.1"
                     /translation="MKGIVLAGGLGSRLNPLTKVTNKHLLPVYNQPMIYYPIRTLINA
                     GIDDIMIVTGGNSAGDFLKLLGNGKQFGLKHVNYTYQEGEGGIADALSLVEHFADDSS
                     ICVVLGDNIIEGNIRQAADDYQRQGKGAKILLKKVPDPNRFGVPVIEGERVVRIEEKP
                     REPKSDYAVVGIYFFDTRVFEIIRGLEPSSRGELEITDVNNDYIERGEMSWSELEGWW
                     SDAGTFDSLQRASNLVAETGANKL"
     gene            complement(3545..4477)
                     /locus_tag="VGB68_09995"
     CDS             complement(3545..4477)
                     /locus_tag="VGB68_09995"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0049606.1"
                     /translation="MELIKGEKIKTHAEYHWIKINCPICDLPPTKFIGKRGGAAHREN
                     LGVEADIWTCGKCGLLFANPMPVPVKGLEQHYSVDADEYFEYHKSDARAATSFMLIEE
                     AEKILGRKGKLLDVGTGRGEIILCAKQRGWEVAGVEPSEGFAAYTEKATGIKIHCDTV
                     ENCGFADAEFDVVILSAVLEHLYNPDEVVAEISRITKRGGLFYLDVPNERGLFFKVGN
                     LYQKIRGTGWSVNLSPTFTPFHIFGFSLKSVKMLLAKHGFGIQKMGVYGGMSLVPAGG
                     GLRAKIESQAAKAITAVSNYGNLGTYIIGWAIKK"
BASE COUNT         1059 a         1253 c         1121 g         1211 t
ORIGIN      
        1 tttatcataa ctggcaaaaa cgaaatccag cccgcgcgcg cccagttcct ttcgtaattt
       61 ttcatttttc gccaaataaa tcaagccgtt taagaatcct tccgcgtcgc cgctcgccgt
      121 gcgaacgcct cgctcgcaca cggtaaagcc gtccgtttca ttttgcctct cagcgccgag
      181 caaatcgatg acgccgccga ccgcggtcga aatacacggg cgcgcgtttg ccatcgcttc
      241 aatcagcgag agcggcgtgc cttcgttcaa agacgtgagc gcgacgatgt ccagcgcggg
      301 atagaaaacg tccgcgtcgc tgcggtttcc caaaaatgtg acaatgtcct ttatattata
      361 cttttccgct tctttttcaa gtttttcgcg aagatgtccg tcgccgatta tgacgaattt
      421 cagaggcgga aagtcgaaat tttctttata tgactgcgcc gcccgcaaaa aaagcgaatg
      481 atttttaatc tccgtcagcc tgccgaccag accgactaat atttcatcgt cgcgcgcgcc
      541 gatttcctcg cgcagcggcg ctcgtttcgc ctgccattcg gcaaacgacg aaaggtcgat
      601 gccgagcggg acgacggcga attgttttgt tttgccgacg ccgaagcggt ggtgaatttc
      661 ctcaaactgc tgccggctga tagtgatgat tttatcggtc gccagccgcg ccagagcttt
      721 ttcgataaaa agaaaaaaac tggttttgcg cctgccgtaa taactgtgaa agacgtgacc
      781 gtgataggtg tgaacgaagc gtacggcgcg cggctgaccg attaaggtcc ggagcgtcag
      841 ccagcgataa aaaaaaccgg cgacgcgccc gattgttcct gcctttgcgg tgtgcgtgtg
      901 aacgatgtcg gggttttctt tttgcagaaa acgataaact ttccagagcg agataatgtc
      961 tttcgccgaa agctcgcggc tcatttccgg gatgaaaacg ggagcgactt cgtgcgcggc
     1021 ggcaaaatag ctcatatctt cctcgccttc ggggacggtg cccgtcatca gcacggactg
     1081 gaattccgcg ttttgcagcg ctttcgtcag ccagacgacg tggcgcgcgg gaccgccgac
     1141 gttgagcctg gcaataatgc gaacaatctt catataagcc ggacgagccg ccagcaataa
     1201 gttttgaatt ccgaaggtta aatttttagt taaaacctga atttaaaatt caaaatccaa
     1261 aatttaaatt tgcccgcggc tcgccgtttc agcagtcgct caaccaactg cggctttgat
     1321 ttttcacaaa gtggcaattg gttatacgag ggatgtggcg cctcgccgcc gaccgcgtaa
     1381 aggaaattta atcgtctatc gtaaaaatcg tttcggggtc gtcttcgtgg cgaatctcgt
     1441 caatcgcctc ttttttacct tcgcccatat aaagccggtt cggacagttt atgacgacgc
     1501 ctttttcggt ctggctgatg tttttataag cgtgcgcgac gcctttcggc acaatcaccg
     1561 cctgcgggct gtccgcgccg acaaacagcg tcatcacata gcgaaacgtc ggcgaatcgg
     1621 cgcgattgtc ccacaagcgc agcttgaaat ttcccgcccc gataaagcaa aacaaatccg
     1681 cctgctcgac gtgttcgtgc ggtccgcgct gcatattcgg ctcggtgacg gaaatatacg
     1741 ccatcgccgg ataaaattcc cggctgattt cgtcgtggcg aaaaagctcc gccagccagc
     1801 cgcgctcgtc cacgaatttt ttgagctgat agacgacgac gtcctcgatt ctgcctttgg
     1861 taaaagtatt ttctttcgcg gtttgttgac tcattttgct ttgggcttag attttagttt
     1921 atttgttttc acgcggcaaa cctttgattt tccggtaaag ccgctgcgaa agagtttcca
     1981 gcaaatcttt tttagccgcc gacgaagccc tctccgtcag cgtcaagacc gccgccagcg
     2041 aagttttgac gtagcccgaa aaaccggtcg tctgtttgaa aatagtgttg tgttccatcg
     2101 ccgtctcgac ttcttcgcgc tctttcgtgt aataataaac cgccagcgat tttcgcgtta
     2161 cgtccggcgg cagattcagc ttgcgcggat gcccgtgata ggaaacctcg ttggtttcaa
     2221 atatcacggc gcggttgaaa acgggcgcga tgttttcgat ttgccgcttt tcctgcatat
     2281 cccagaattc cagatagcct tcgtattcgc gcttccagtc tttgttcaga tacaaaagca
     2341 ggttcaggcg gcgatgctct ttcgtgcgcg ggtgaaaatt ataatcgacg tggacgtcca
     2401 gatagccgcc gttcattatt tgatgcaagc cgccgccgct caaagtcggg tcggcaatca
     2461 aatcgggaat ccggcagatt gacgaaacca gattgcggaa ttcttcggtt tcggtcatcc
     2521 gaaaaaaatc gccgatcggc gcgggaaaat tcttgttgag cgtaagtttt ttgagctggt
     2581 gaatataggg cgtgtcttcc cacgcgccgc cggtcggcgt cgggtaagcc gccagaattt
     2641 cttcggcgaa atccgctgtc agaaaatcgt caatcacaac ataacgaaac ggcttcgccg
     2701 cgtcccacgc gctttgaagc tcgctcaatc tgtcaggcgt gatatttaag atagtttcgt
     2761 ttgacatcag attttatata gtaataaacc cgcgcgttat ccgattataa tttatttgct
     2821 ccggtttcgg cgaccagatt tgaggctcgc tgcaaactgt cgaacgttcc ggcgtccgac
     2881 caccagcctt ccagctcgct ccacgacatc tcgccgcgct cgatataatc gttgttcacg
     2941 tccgtaatct ccaactcgcc gcgcgacgaa ggctcgagcc cgcgaataat ttcaaagacg
     3001 cgcgtatcaa aaaaataaat cccgacgacg gcgtaatccg atttcggctc tctcggcttt
     3061 tcctcgatgc gcacgacgcg ctcgccttcg atgacgggca cgccgaagcg attcgggtcg
     3121 ggaacttttt tcagcagaat cttcgcgcct ttgccctgcc tttgataatc atcggcggct
     3181 tgccggatat tgccctcgat aatattatcg ccgagcacga cgcaaatcga cgaatcgtcg
     3241 gcgaaatgct cgaccagcga aagcgcgtcg gcaatgccgc cttcgccttc ctgataagtg
     3301 taattgacgt gctttaagcc gaattgcttg ccgttgccga gcaatttcag aaaatctccg
     3361 gcggagtttc cgcccgtgac aatcataata tcgtcgattc cggcgtttat cagcgtccga
     3421 atcggataat aaatcatcgg ctgattgtaa accggcagca agtgcttatt tgtaactttc
     3481 gtgagcgggt tcagccttga gccgagtccg cccgctaaaa caataccttt cataaactaa
     3541 aaaattattt ctttatcgcc cagcctatta tataagttcc gagatttccg taattgctga
     3601 cggcggtaat ggctttcgcc gcctgacttt cgattttcgc gcgcagtccg ccgcccgccg
     3661 gaaccaggct cataccgccg taaactccca ttttctggat gccgaacccg tgtttcgcca
     3721 gcagcatttt taccgatttc aggctgaaac cgaaaatgtg aaacggcgtg aaagtcggcg
     3781 aaagattgac cgaccagccc gtgcctcgaa ttttctgata taaatttccg accttaaaaa
     3841 acaagccgcg ctcgttcggc acgtccagat aaaaaagtcc gccgcgtttt gtaattctcg
     3901 aaatttcggc gacgacctcg tccggattat atagatgctc caaaaccgcc gaaagtatca
     3961 cgacgtcgaa ttccgcgtcc gcaaaaccgc agttttctac cgtatcgcaa tgaattttaa
     4021 tgcccgtcgc tttttccgtg taggcggcaa agccttcgga cggctcgacg cccgccactt
     4081 cccagcctct ttgtttggcg cacaaaataa tctcgccgcg tccggttccg acgtcgagca
     4141 gctttccttt tctgccgaga attttttccg cttcttctat cagcataaac gaggtcgccg
     4201 cgcgcgcgtc cgatttatga tattcaaaat actcgtcggc atcgaccgaa taatgctgct
     4261 ccaaaccttt gaccgggacg ggcatcgggt tggcaaacag caagccgcat tttccgcacg
     4321 tccagatgtc ggcttcgacg ccgaggtttt cgcgatgcgc cgcgccgccg cgttttccga
     4381 taaatttggt cggcggcaaa tcgcaaatcg gacaattgat ttttatccaa tgatattctg
     4441 cgtgagtttt aattttctcg cccttgataa gttccatttc aagcttatcc gtagatatat
     4501 tttataaatt ttaatgttac gacgttccgg tttttttgta aaaaacaata aagtcagagc
     4561 ttagacgcga ggcgacttgt ttgtttttga ttttaccgcc gagcttttgc gccagattca
     4621 cggacaattt cggaaacacg taac
//
LOCUS       DASWNE010000505         7133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_328466, whole genome shotgun sequence.
ACCESSION   DASWNE010000505 DASWNE010000000
VERSION     DASWNE010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7133
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328466"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3694
                     /locus_tag="VGB68_10000"
     CDS             <1..3694
                     /locus_tag="VGB68_10000"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HEY0049607.1"
                     /translation="NDIRIPEVITPDVWRNKIEFLTPPSDVKRSDILRSFIKQNNELV
                     GMTNQQIDSLKTFADYTNPDGNLSFVELNQEINGIPVFRGEVKAGFAKNGQMIRVVNN
                     LAPGLDYNSLSADFRNPVDAVKAAARHINHELTPADTTRNEAESNNLKVTFGTGDSAT
                     TAEKMYFPTEPGVAVPAWRVTLYQKVDAYYVMVDAEIGTMLWRKNLVADQTQSATYNV
                     YANPNAFINVADNPAPLSPGPINPTLGTQGAVISRTNVTRIGNEAPFTFNNNGWINDG
                     QNITDGNAVEAGLDVVAPDGVDAPVTGDPNRTFTSLWNPPPGNPAPGDAPTATEARRG
                     AVIQMFYAVNWWHDEMYRLGFTEQARNFQHDNFGRGGLGNDRIRAEGQDSSGTNNANF
                     LTNADGTRGRMQMYTWTGPTPDYDGTGDAEVIIHEVSHGLSNRLHGNNSGLGGQGGMM
                     GEGWGDWYALAMLSEPTDPINGVYALGGYATHLAAAGYTSNYYHGIRRFPKAVIAFTG
                     PNGLPHNPLTFRYLNTGCDTLVGTTTTNPNSAYPRGPFGTTGSCAQVHNAGEIWSSAL
                     WEVRALMITRLGWATGNRRVMQVVTDGMKLAPLNPTFLQERDAIITAGAALPLAPEAS
                     ADVADIREGFRRRGMGFSASVQSSTAVTEAFDFPNVVMTNPFSVSDSTGDNDGYPEPG
                     ENVLLSVAVTNTTGSTINNVVATVAGGGSANYGNIADGATVTRQIPYTVPAAAICGNM
                     HQVSITVSSDAGAQLPQTREFRLGAPVGGAPATFSNTAAVTINDNAAATPYPSNVTVS
                     GLTGNKVIRLNLSGLSHTFPADVDILLVGPGGQKFMVMSDLGGSDDVANINVSLTDTA
                     TSALPTSLLTSGTYRPGNSDTTTDVMPAPAPAAPYSNPAPAGTATFASTFGSDGSTMN
                     GTWALYVRDDAGTDVGAMTGGWSLTFEANDFVCVADPGATPKSRADFDGDGKTDLSVF
                     RPSEGNWYMQRSTAGFSAVQFGASADTIVPADYDGDGKTDIAVFRNGTWFRLNSSNGA
                     FSAPSFGQAGDIPQAADFDGDQKADLVVFRPSTGDWYRINSSNGAFDKQHFGIAGDKP
                     VVGDYDGDSKADVAVFRPSNATWYRLNSNGGALVIAQFGLATDMPVPADYNGDNKDDL
                     AVFRPSNGTWYIARTTGVPSQNFDTVQFGANGDVPVPGDYDGDGKDDQAVYRGGAWYL
                     NRSTSGFSAANFGLASDRAVPKSYIP"
     gene            3904..4497
                     /locus_tag="VGB68_10005"
     CDS             3904..4497
                     /locus_tag="VGB68_10005"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0049608.1"
                     /translation="MEAVKALAGSQGADLVRRCRAGDGTAWEEIVATFSRRIFNLAYR
                     FTSSVDAAEDLTQEVFVRIYRTLDQYDPKHGDLSNWLMRLARNLIIDDYRHRQRNPQD
                     SMADDVDDHAFHLRSVGGSVHKEIERRELARQVQEGIDKLPPDLRTCVILRDIEELSY
                     QEIVELLKIPEGTVKSRINRGRIELAKILRRMRIVSM"
     gene            4566..5354
                     /locus_tag="VGB68_10010"
     CDS             4566..5354
                     /locus_tag="VGB68_10010"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY0049609.1"
                     /translation="MTYENEKIINCSEFEGLLTDYLDGTLARETHKAVAAHALRCPLC
                     HALLNEVKSALEVCHEIAVPKAPLTRLEARIISMTMPETAMACDEFEKHLTDYLDGFL
                     PATVFHRWERHAVLCDRCTDLPGEVVRSIAACYTYKTEELPLPAGLHERILQETLGTT
                     KAGTLKSSWVAQVREWARALSLPISLPQLAPVAMMLVFAVMVLTQTVSADGSLSGMYQ
                     KSFELAGQTYKQGADIMLGGKPAEVNQYNQEPIQGDYVNQEGKQ"
     gene            5355..6119
                     /locus_tag="VGB68_10015"
     CDS             5355..6119
                     /locus_tag="VGB68_10015"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HEY0049610.1"
                     /translation="MNCAYHAHNTAVVNCNGCGKPLCPACDHRIKGFPFCQDCIVTGV
                     ELLRSRNQSSYVPYVKRQVSPFIATILSLICPGLGAAYNGQTSKGLVHFAVFVGLFQL
                     ATMVGMPLFVLGFVGMWLYAAVDAWRTAQLIRSGLTPDGAEDWIVQRFKGNPKVFGIV
                     LTALGAMFFLQMFLPMRSFMRAVMPVLLIMLGIYLLREYVFKPKAKETDWTEFSTRAD
                     APGFAAGAIGETNFRTGDYGAVEDEFETQVKSWKSR"
     gene            6275..6553
                     /locus_tag="VGB68_10020"
     CDS             6275..6553
                     /locus_tag="VGB68_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049611.1"
                     /translation="MANWLSTTAKFGGGIAALALGGTLVYATATGGWGSLLVLLALII
                     ALVKQIIAFIGFLTFAIKAVIVLSFVALILGVAVMIFRSWGKNRRSKE"
     gene            6870..>7133
                     /locus_tag="VGB68_10025"
     CDS             6870..>7133
                     /locus_tag="VGB68_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049612.1"
                     /translation="MHQDDRFYTFLLTHSSKTKIYIRRVEISKKFVTNFSASLIFTIG
                     LLSIGVLGLLKSDDIVLNKVGAQVLSKSETTTERAGFSRNNPYI"
BASE COUNT         1631 a         1893 c         1974 g         1635 t
ORIGIN      
        1 caatgacatc cgcattcccg aagttattac gcccgacgtc tggagaaaca aaatcgaatt
       61 cctgacgccg ccctcagatg tgaaacgctc ggatattctc cgcagcttta tcaaacaaaa
      121 caatgagtta gtcggaatga cgaatcagca gattgattcg ctgaaaacat ttgccgatta
      181 caccaatccg gacggcaatt tgtcgttcgt cgaactgaac caggaaatca acggcattcc
      241 cgtgtttcgc ggcgaagtca aagccggttt tgccaaaaac ggtcagatga ttcgcgtcgt
      301 caacaacctc gcgccgggct tggattataa cagtctctcg gcggatttcc gcaatccggt
      361 cgatgcggtc aaagcagccg cgcgtcacat caatcacgaa ctgacgcccg cagacacgac
      421 gcgcaacgaa gcggaatcga ataacctgaa agtcaccttc ggaacgggcg attcggcgac
      481 gacggctgag aaaatgtatt ttccgaccga gccgggcgtt gccgttcccg cgtggagagt
      541 cacgctttat cagaaggtcg acgcttacta cgttatggtc gatgccgaaa tcggcacgat
      601 gctctggcgc aagaatttgg ttgcagacca gacgcagtcg gcgacctata acgtttacgc
      661 caacccgaac gcgtttatca acgttgccga taacccggcg ccgctgtcgc cgggtcctat
      721 caacccgacg ctcggaacgc agggagcggt tatcagcaga accaatgtta ccagaatcgg
      781 taacgaagcg ccgttcacgt tcaacaacaa cggctggatt aacgacgggc agaacattac
      841 cgacggtaac gcggttgaag ccggtcttga cgtcgtcgcg ccggacggcg tcgatgcgcc
      901 tgtaaccgga gacccgaacc gcacgtttac ttcattgtgg aatcccccgc cgggaaatcc
      961 cgcgcccgga gatgctccga ccgctactga agcgcggcgc ggcgcggtca tccagatgtt
     1021 ttacgcggta aactggtggc acgacgaaat gtatcgcctc ggctttaccg aacaggcgcg
     1081 caacttccag cacgacaact tcggtcgcgg cggcttgggc aacgaccgca tcagagctga
     1141 aggtcaggac tcgtcgggca cgaacaacgc caacttcctg accaacgccg acggtacgcg
     1201 cggacgtatg cagatgtaca cgtggactgg tccgacgccg gactatgacg gaacgggcga
     1261 cgccgaggtc atcatccacg aagtctcgca cggactctcg aaccgtctgc acggcaacaa
     1321 cagcggttta ggcggtcagg gcggcatgat gggcgaaggc tggggcgatt ggtatgctct
     1381 cgcgatgctg tcggagccga ccgacccgat taacggggtt tatgccctgg gcggctacgc
     1441 gacgcactta gccgcagccg gctatacatc aaactattat cacggcatcc gtcgtttccc
     1501 gaaagcggtt atcgctttca ccggaccgaa cggtttaccg cacaacccgc tgacgttccg
     1561 ctacctgaat acgggctgcg acacgcttgt cggaacgacg acgaccaatc cgaacagcgc
     1621 ttacccgcgc ggaccgttcg gcacgaccgg ttcgtgcgct caggttcaca acgcgggcga
     1681 aatctggagc agcgctctgt gggaagtccg cgccctgatg attacccgtc tgggctgggc
     1741 aaccggcaat cgccgcgtga tgcaggtcgt caccgacggc atgaagctcg ctccgcttaa
     1801 cccgaccttc ctgcaggaac gtgatgccat catcactgcc ggagcggctc tgccgctcgc
     1861 gccggaagca tcggctgatg tggctgatat tcgcgaaggc ttccgccgtc gcggtatggg
     1921 cttctcggca agcgttcagt cttcgacggc tgtcaccgaa gcgttcgatt tcccgaatgt
     1981 ggttatgact aacccgttct cggtcagcga ctcgacgggc gacaacgacg gctatcccga
     2041 accgggcgaa aacgttttgt taagcgtggc ggtcaccaac acgaccggct cgaccatcaa
     2101 caacgtcgtt gccacggttg ccggcggcgg cagcgccaat tacggcaata tcgcggacgg
     2161 cgcgaccgtt acccgtcaaa ttccgtatac ggttccggca gcagccattt gcggaaacat
     2221 gcatcaggtt tcgattaccg tcagcagcga tgcaggcgcg caattgccgc aaaccagaga
     2281 attcagactc ggcgcaccgg tcggcggcgc accggcgacg ttttcgaaca cggcggcggt
     2341 caccattaac gataacgccg cggcaactcc gtatccgtcc aacgttacgg tttccggtct
     2401 gaccggcaac aaggtcatca gacttaatct tagcggtttg tcgcacacct tcccggctga
     2461 cgtcgatatt ctgttagtcg gtccgggcgg acagaagttt atggttatgt ccgatttggg
     2521 cggcagtgac gatgtcgcga atattaacgt gtcgctgacc gatacggcga ccagcgcgtt
     2581 gccgacatcg cttttgacaa gcggcacata cagaccgggc aacagcgata cgacaacgga
     2641 tgttatgcct gctccggcac cggcggctcc gtatagcaat ccggcaccgg ctggcacggc
     2701 gacctttgcc tctacattcg gttcggacgg ttccacgatg aacggaacct gggcgctgta
     2761 tgtcagagac gacgccggca ccgacgtcgg agcgatgacg ggcggctggt cactgacctt
     2821 tgaagccaac gatttcgttt gtgtggctga cccgggcgcg acgcctaaat cgcgcgccga
     2881 ctttgacggc gacggcaaaa ccgacttgtc ggtcttccgc ccgtcggaag gcaactggta
     2941 tatgcagcgc tcgactgccg gcttcagcgc cgttcagttc ggtgcttctg ccgatacaat
     3001 cgtcccggct gattacgacg gcgacggcaa gactgatatt gccgtgttcc gcaacggtac
     3061 gtggttccgt ctgaattcgt cgaacggcgc gtttagcgct ccgtcattcg gtcaagccgg
     3121 cgatattccg caggcagccg atttcgacgg cgaccagaaa gccgaccttg tcgtcttcag
     3181 accttcgacc ggcgactggt atcgcatcaa cagctcgaac ggagcgtttg acaaacagca
     3241 tttcggcatc gcgggcgata agcctgtggt cggagactac gacggcgatt cgaaagccga
     3301 cgttgccgtc ttccgtccgt cgaatgcgac gtggtatcgt ttgaattcaa acggcggcgc
     3361 gcttgtcatt gcgcagttcg gtttagcgac cgatatgccg gttccggcgg attataacgg
     3421 agacaataag gacgacctgg ctgttttccg cccgtcaaac ggaacctggt atatcgccag
     3481 aacgaccggc gttccttcgc agaatttcga caccgtccag ttcggcgcga acggcgacgt
     3541 tccggttccg ggcgactacg acggcgacgg taaagacgat caggctgtct atcgcggcgg
     3601 tgcctggtat ctgaatcgtt cgacgagcgg attctcggct gccaacttcg gtttggcatc
     3661 tgacagagcc gttccgaaat cctacattcc gtaaattcag gcacgataat cggaaagctg
     3721 aagtttaatt tttcgtttat ccttaaatcg agaaaggctg atggttgaaa taatcgaccg
     3781 tcagcctttc tttctttctt tggaactttt ttcggcgacc gttcgtaaga actgtttgac
     3841 agagcgagat ttgagctttg ttgctttgaa cggaaagcat ttgtttttcg gagaaatcaa
     3901 acattggaag ctgtaaaagc attagctggc agtcagggag ctgacctcgt gcggcgttgt
     3961 cgcgccggag acggcacggc ttgggaagag attgtggcga cgttttcgcg ccgcattttc
     4021 aatcttgcct atcgctttac gtcaagcgtc gatgcagccg aggatttaac gcaggaggtc
     4081 tttgtcagga tttatcgcac gctcgaccaa tatgacccga agcacggcga tttgtcgaat
     4141 tggctgatgc ggctggcgcg aaatctgatt atcgacgatt accggcatcg ccagcgcaac
     4201 ccgcaggatt cgatggcgga cgacgtggac gaccacgcgt ttcacctgcg ctcggtcggc
     4261 ggctcggtgc ataaagaaat cgagcggcgc gagctggcgc gacaggtgca ggaaggaatc
     4321 gacaagctgc cgcccgattt gcgaacttgc gttatcctgc gcgacatcga agaattgagc
     4381 tatcaggaaa tcgtcgaatt attaaaaatt cctgaaggca cggtaaaatc gcgcattaac
     4441 cggggacgaa tagaactggc gaaaattttg cgccggatgc gcatcgtgag catgtagcaa
     4501 aacgaatatg aaaatagaaa tgtccggtta gaaaagtttt ggggaaaatt gttttaggtt
     4561 gttttatgac ttatgaaaac gaaaaaatca taaattgctc agagtttgaa ggtcttttga
     4621 ccgattatct ggatggcaca ctggcgaggg aaacgcacaa ggcggtcgcc gcccacgcgc
     4681 ttcgctgtcc tctgtgtcat gcacttttaa acgaggtcaa aagcgcgctc gaagtctgtc
     4741 acgaaatcgc cgtgccgaaa gcgcctttaa cgaggctcga agcgcggatt atttcgatga
     4801 cgatgcctga gacggcgatg gcgtgcgatg aattcgaaaa acatctgacc gattatctgg
     4861 acggatttct gcccgcgacc gttttccatc gctgggagcg tcacgccgtt ttgtgcgacc
     4921 gctgcacgga tttgccgggc gaagtcgtgc gctcgattgc ggcttgctac acatacaaaa
     4981 cggaagaatt accgctgccc gccggtctgc acgaaagaat tttgcaggaa acgctcggca
     5041 cgacgaaagc cgggacgctc aaatcttcgt gggtcgcgca ggtcagggaa tgggcgcgcg
     5101 cgctgtcgct gccgatttcc ttgccgcagc tggctccggt ggcgatgatg ctggtttttg
     5161 ccgtgatggt tttgacgcag acggtctccg ccgacggaag cctgagcgga atgtatcaaa
     5221 agagtttcga gcttgccggt caaacgtaca aacaaggcgc ggacattatg ctcggcggaa
     5281 aaccggctga agtaaatcaa tataatcagg agccgattca aggagattac gtcaatcaag
     5341 agggtaaaca gtaaatgaat tgtgcatatc acgcccataa tacggcagtt gtcaattgta
     5401 acggatgcgg aaagcctctg tgcccggcgt gtgaccatcg catcaagggc tttccgtttt
     5461 gtcaggactg catcgtcacg ggcgtcgagc ttctcagaag ccgtaatcaa tcttcatacg
     5521 ttccgtatgt caaaagacag gtttcgcctt ttatcgccac gattttgtcg ctgatctgtc
     5581 cgggcttggg cgcggcttac aacggtcaga cttcgaaggg attggttcat ttcgccgtct
     5641 ttgtcgggct ttttcaactg gcgacgatgg tcggtatgcc gcttttcgtc ctgggttttg
     5701 tcggaatgtg gctgtatgcg gcggttgacg cgtggcgcac ggcgcagctt attcgctcgg
     5761 gattgacgcc ggacggcgcg gaagattgga ttgtccaacg ctttaaaggc aatccgaaag
     5821 ttttcggcat tgttctgacg gcgctcggcg caatgttctt tttgcagatg tttttaccga
     5881 tgcgctcatt tatgcgggcg gttatgccgg ttttgcttat tatgctcggc atttatcttt
     5941 tgcgcgaata cgtttttaaa ccgaaggcga aggaaacgga ctggacggaa ttttctacgc
     6001 gcgccgacgc gcccggcttt gccgccggag ccatcggcga aacaaatttt cgcaccggcg
     6061 attacggcgc tgtcgaggac gaatttgaaa cgcaggtcaa atcctggaaa tcgagataac
     6121 tttttcggca aaccgaaagc gaaaggcacg catctgattg cttatacctg accgcttcga
     6181 ctaaaatgaa aaatggacgg cgttagttgt ttttttaata ataagctgtc cggcgagaaa
     6241 ataaaccggc aattttaaaa ggatttataa aattatggcg aattggttaa gcacgacggc
     6301 aaaattcgga ggcggcattg ccgctctggc tttaggcgga actttggtat atgcgacggc
     6361 gacgggcggc tggggaagcc ttctggttct tctcgcgctg atcatcgcgc tggtcaaaca
     6421 gataatcgcc tttatcggct ttctgacgtt tgccattaaa gccgttattg tgctgtcttt
     6481 tgtcgcgttg attctcggcg tcgcggtgat gattttccga agctggggca aaaatcgccg
     6541 ctcgaaagag taatttaaat ttattaaact cagtagttta gaaacggctg tcgcgcaaaa
     6601 tgcccgtctt tacttgattt taacgacagc cctgtgttag ctttaaactt gaccgtactt
     6661 ttaaataatc tatatataga ttattcaacc aaataaataa tccacaattt gattattttg
     6721 acccccagag agaggttttg cgttcgtttt ttttcggaca cggcttctca gtaaggatcg
     6781 cgtcagtcgc ccgtaggctg tgctttcgtg attccccgcg cggcagaagt tttcgccggc
     6841 gcgagaattc ataaaaagga gcttatttaa tgcatcagga cgaccgcttt tatacgtttt
     6901 tattaacgca ctcgtctaaa accaaaattt acatccgccg agtcgaaatc tcgaaaaagt
     6961 ttgtaaccaa tttttccgca agtttgatat ttacaatcgg acttctttcc atcggagtcc
     7021 tcggacttct aaaatccgac gatatcgtct taaacaaagt cggagcgcaa gttttatcca
     7081 aatcggaaac caccaccgaa cgcgccggtt tttcccgaaa caatccatac atc
//
LOCUS       DASWNE010000506         2963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_194039, whole genome shotgun sequence.
ACCESSION   DASWNE010000506 DASWNE010000000
VERSION     DASWNE010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194039"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VGB68_10030"
     CDS             <1..601
                     /locus_tag="VGB68_10030"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HEY0049613.1"
                     /translation="AVIRAILLDPEARGNIKTEPRYGKLREPVQLLTNVLRRFNVRSA
                     DGTQLSDGNVNGQSSAMGQNAFNSPTVFNYYSPDYIVPGTTVLAPEFGIYTTGTSIAR
                     ANFANTMIFSRINVAQPNTPLGTSISLADMQALAAADATGNQLMDALNQKMMHGAMSS
                     QMRTTILNAVLAAPSTNPLLRAQTAVYLIATSSQYQVQR"
     gene            658..2091
                     /locus_tag="VGB68_10035"
     CDS             658..2091
                     /locus_tag="VGB68_10035"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEY0049614.1"
                     /translation="MVTLATQVQHFGMMSALAQTVDDREAEGGSDYRALICIFMAGGN
                     DGNNMVIPNHNDANLSNYTAYSNARATQGLAIPQNQLLPVSVPRMNGLSYGLHPSFGT
                     VAGGINNGIHELWSLGKLGVVTNVGTLVRPTTKAQYQTASHPKPYQLFSHSDQVSQYQ
                     SARSDTQSFTGWGGRVSDRRTQPANPSGLIPMITSISGAQLFTAGQTTLPLAIAAAPT
                     GLNQVLNPQGFNTTAPSVARLAAFNNLRTMDLSSDLVAAASHVTDSAIAANAQLQISQ
                     EVTVAFPNTSLGNQLKQVARMIKKKDDLNINRQIFFCQIGGFDTHNNQQNATNGQVAL
                     LANLSQAMRAFYDEMVVQGLSDKVTAFTMSDFSRTMNPAGSGAAVGTDHAWANHMFVL
                     GGALTASDFYGVNTTNGTPFPTLAFNGPDDADSGTNARGRWIPTTSVEQYAATLARWY
                     GLPETDMSGVFPLIGNFPTSNLGFMQP"
     gene            complement(2504..>2963)
                     /locus_tag="VGB68_10040"
     CDS             complement(2504..>2963)
                     /locus_tag="VGB68_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049615.1"
                     /translation="VMFDGFGNKLEKRYFKGHPRLHSVLIRTAVDGRREILVYGQNGE
                     KNSVDGELGKRLLSASADEIANAAKIYESRPVIRNRAITVQNEQTKPQPPVSTSDALP
                     QQPAPTPPNETTVEEPIRETPENKDSNEIPPTEPQKEEAKQGKKEDKENR"
BASE COUNT          666 a          793 c          803 g          701 t
ORIGIN      
        1 agccgttatc cgggcgattc tgctcgaccc ggaagcgcgc ggcaacatta aaaccgagcc
       61 gcgttacgga aaattgcgcg agccggtgca attgttgacg aacgttctgc ggcgttttaa
      121 tgtccgctcg gcggacggaa cgcagttgag cgacggaaac gtcaacgggc aatcgagcgc
      181 gatgggacaa aacgctttca attcgccgac ggttttcaat tattattcgc cggattacat
      241 cgttccgggg acgaccgtgc tggctcccga attcggaatt tacacgaccg gcacttcaat
      301 cgcccgcgcg aattttgcca acacgatgat tttcagtcga ataaacgtcg cccagccgaa
      361 cacgccgctc ggaacgtcaa tcagcctcgc cgatatgcag gcattggcgg cggctgacgc
      421 gaccggaaat caattgatgg acgctctgaa tcagaaaatg atgcacggcg cgatgtcttc
      481 gcagatgcgc acgacgattc tgaacgcggt tctcgccgcg ccgtcaacca atccgctgct
      541 gcgggctcag acggcggttt atttaatcgc gacttcgtcg caatatcagg tgcagaggta
      601 aaaactatga aacagacaag acgtgaattt ttaatgaaat caggttgcgc cttgagcatg
      661 gtaaccttgg cgacgcaagt gcagcatttc ggaatgatga gcgctctggc gcagacggtc
      721 gacgaccgcg aagccgaggg cggaagcgat taccgagctc taatctgcat ttttatggcg
      781 ggcggaaatg acggcaacaa tatggtcatt ccgaatcata acgacgccaa tctgagcaac
      841 tacacggctt attcaaacgc gcgagcaacc caaggactgg cgattccgca aaatcagctt
      901 ttgccggtca gcgttccgcg gatgaacggc ttgagctacg gcttgcatcc gagcttcgga
      961 acggtggctg gcggcattaa caacggtatt cacgagcttt ggagtctggg caagctgggg
     1021 gtcgtgacga atgtcggaac gctggttcgc ccgacgacga aagcgcagta tcaaacggct
     1081 tcgcacccga aaccgtatca gcttttctcg cattccgacc aggtttcgca gtaccagagc
     1141 gcgcgctccg acacgcagtc ttttacgggc tggggcgggc gcgtttccga ccgccggacg
     1201 cagcccgcca atccgagcgg attgattccg atgataactt ccatctccgg cgcgcagctt
     1261 tttacggcgg gacagacgac tctgccgctc gccatcgccg ccgcgccgac cggattgaat
     1321 caggttttaa atccgcaggg cttcaatacc accgctccgt cggtagcgcg gctggcggcg
     1381 tttaacaatc tgcgcacgat ggacttaagc tcggatttgg ttgccgcggc gagccacgtc
     1441 accgacagcg cgattgcggc aaatgcacag ctgcaaattt ctcaggaagt gacggtcgct
     1501 ttcccgaaca ccagcctcgg aaaccagctc aaacaggtcg cgcggatgat aaaaaagaaa
     1561 gacgatttga acatcaaccg gcaaatcttt ttctgccaga tcggcggttt cgacacgcac
     1621 aataaccagc agaacgcgac gaacggacaa gtcgccctgc tggcaaattt gagtcaggcg
     1681 atgcgcgcgt tttacgatga aatggtcgtg cagggcttgt ccgacaaggt gacggctttt
     1741 actatgtccg acttttcgcg aacgatgaat ccggctggtt cgggcgcggc ggtcggcacc
     1801 gaccacgcgt gggcaaatca tatgttcgtg ctcggcggcg cattgacggc gagcgatttt
     1861 tacggcgtta acaccaccaa cggaacgccg tttccgacgc tcgcctttaa cggtccggac
     1921 gacgccgaca gcggaacaaa tgcgcgcggg cgctggattc cgacgacttc ggtcgagcaa
     1981 tacgccgcga cgctggcgcg ctggtacggc ttgccggaga ccgatatgtc gggcgttttt
     2041 ccgttaatcg gaaacttccc gacttcgaat ctcggtttta tgcagcctta aaatcgtctt
     2101 ttcagagcgc aatttaaaag gcgaaagttt atcgactttc gcctttttta tgaataacga
     2161 gaggcttttg tcatctgccg gataagtttc gttattcggg aatgaatacc cgattcaggc
     2221 aataaagaca gaaccgcgac tatcggcgag cgcggctctt aacctgaaga aaacgctaag
     2281 aaaacgctcc gtttgcaatc cgcgtttctg cctgcgcctt atttcggtga aattcgctct
     2341 aatctaatcg ctttgcacaa aaacaaggaa aagtcgggaa ggatttccgt cgctgattgc
     2401 agattgtaaa tttgtaatta taaatagctc ggcaaaagat attgaatatc aatatagaat
     2461 ttagaattta caatattcac tttctttatt ttcgggcaaa accttatctg ttctctttgt
     2521 cttctttttt tccctgtttt gcttcttctt tctgcggctc ggtcggcgga atttcgtttg
     2581 aatccttatt ctccggcgtt tcccgaatcg gttcttcgac cgttgtttcg ttgggcggtg
     2641 tcggcgcagg ctgctgcggc agcgcatcgg aggtcgaaac cggcggctgc ggtttcgtct
     2701 gctcgttttg aacggttatt gcccggtttc tgataacggg tcggctttcg taaattttcg
     2761 cggcattggc tatctcgtcg gcggaagcgc tcaacagacg tttgcccagc tcgccgtcga
     2821 ccgaattttt ctcgccgttt tgtccgtaaa ccaaaatttc gcgtcgcccg tcgacggcgg
     2881 ttctgatgag gacgctgtgc agacgcggat gtcctttgaa ataacgtttt tccagcttgt
     2941 ttccgaaacc gtcgaacatc acc
//
LOCUS       DASWNE010000507         1906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_613417, whole genome shotgun sequence.
ACCESSION   DASWNE010000507 DASWNE010000000
VERSION     DASWNE010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613417"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(387..1448)
                     /locus_tag="VGB68_10045"
     CDS             complement(387..1448)
                     /locus_tag="VGB68_10045"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEY0049616.1"
                     /translation="MNLEIKPKKNCRWDFLSLGEVMLRFDPENDRIHNTRSFRVWEGG
                     GEYNVARSLSKVFRLDAGIVTALADNQIGRLVEDLIWQGGVDATEILWRETDGISRKV
                     RNGMYFIERGFGLRSPVGCSDRGNTAVSQLKVNDIDWQRIFSTNGARWFHTGGIFAGL
                     SETTADVALEAMRKARENGAIVSYDLNYRDSLWSERGGRRAADALNQNLLPFADVVFG
                     VENFKAGFADYNEEIFARAAGEIVSRFPNLKAIATTLRDVKSASRHDLGAVCFADGKI
                     FKANDFADLEVLDRVGSGDAFAAGLIYGLLAGKDFEYAVNAGAANSALTMTTLGDGSS
                     ATLAEVEKLVHSRELNLSR"
     gene            complement(1497..>1906)
                     /locus_tag="VGB68_10050"
     CDS             complement(1497..>1906)
                     /locus_tag="VGB68_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005883581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0049617.1"
                     /translation="SSIFRLSQAAGRKMIEQGAGKIVNIASLLSFQGGINVPAYTASK
                     SGVAGLTKAFANEWAKHNVNVNAIAPGYMATNNTAALRADETRNRQILERIPAGRWGA
                     PDDLTGAAVFLASKASDYLQGHILVVDGGWMAR"
BASE COUNT          483 a          518 c          442 g          463 t
ORIGIN      
        1 agaaatagag cggtaaaaaa atgaaagcac gagatgataa ttaaatcatc tcgtgctttt
       61 ttaatatgaa ttccgggaac tatttttatc tttaagacgg ctttgcacaa aaataaagaa
      121 agggaaaatt gtaaattcta aattgtaaat tctaaattga tgttcggtat cttttgttaa
      181 acaatttaga attaataatt gagaatttac aatcagccat cggaattttt ctcgattttc
      241 tttatttttg tgcaaagcac tttaagacaa aagaacacaa cgaggaaaaa tgcgcggaaa
      301 tgaccggatg aactgatgat atatgaaaat cgtctttctt gttgctgtta actctttttt
      361 gtctctctct cggcggcgaa ttcccgttac cgggaaagat tcagctcgcg gctgtgaact
      421 aatttttcca cttccgccag cgtcgccgac gaaccgtcgc cgagcgtcgt cattgtcaat
      481 gccgaattgg cagcgcccgc gttgaccgca tattcaaaat ctttaccggc taacaaaccg
      541 taaatcaaac cggcggcaaa agcgtcgccg ctgccgacgc ggtcgagcac ttccaaatcc
      601 gcaaaatcgt ttgctttaaa aattttgccg tcggcaaaac aaaccgcgcc caaatcgtgt
      661 cgcgatgccg atttcacgtc gcgcagggtt gtggcgatcg ctttcaaatt cggaaatcgg
      721 gaaacgattt cgcccgcggc acgggcaaaa atttcttcat tataatcggc aaaaccagcc
      781 ttgaaatttt cgactccgaa aacaacgtcg gcaaacggca gaaggttttg attaagcgca
      841 tcggcggcac ggcgaccgcc gcgctccgac cagagcgagt cgcgataatt caaatcgtaa
      901 gaaacaatcg cgccgttttc tctcgccttg cgcatcgctt ccagagcaac gtcagccgtc
      961 gtttccgaaa gcccggcaaa aattccgccc gtatgaaacc agcgcgcgcc gttcgtgctg
     1021 aaaatcctct gccaatcaat atcgttaact ttgagctgcg aaaccgccgt gtttccgcgg
     1081 tcggaacagc cgacgggcga gcgcaagccg aaaccgcgct cgataaaata catcccgttt
     1141 cgcactttgc ggctgatgcc gtcggtttcg cgccacaaaa tctcggtcgc atcgacgccg
     1201 ccctgccaaa tcaaatcttc gaccaatctg ccgatttgat tatccgccag agccgtcact
     1261 atgcctgcgt cgagccggaa aactttgctc aggctgcgcg cgacattata ttcgccgccg
     1321 ccctcccaga cgcggaatga gcgcgtgttg tgaatgcggt cgttttccgg gtcaaagcga
     1381 agcatcacct cgccgagcga gagaaaatcc catcgacagt tttttttcgg tttaatttct
     1441 aaattcatta ccaaacgacg aaacctttat cctttatccc ttacccttta cccttttcac
     1501 cgcgccatcc agccgccgtc aaccaccaga atatgacctt gcagataatc gctcgccttt
     1561 gaggcgagaa aaaccgccgc gcccgtcaaa tcgtcgggcg cgccccagcg tcccgccgga
     1621 attctttcta aaatctgccg gttgcgcgtt tcgtcggcgc gcagcgcggc ggtgttgttc
     1681 gtcgccatat aaccgggcgc gatggcgttg acgttgacgt tatgcttcgc ccattcgttg
     1741 gcgaaagctt tggtcaagcc agccacgccg gatttggaag ccgtgtaagc gggaacgttg
     1801 attccgccct gaaacgagag aagcgaagcg atattcacga ttttgcccgc gccctgctcg
     1861 atcatttttc gcccggcagc ctgcgacagg cgaaaaatgc tggaaa
//
LOCUS       DASWNE010000508         4787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_265187, whole genome shotgun sequence.
ACCESSION   DASWNE010000508 DASWNE010000000
VERSION     DASWNE010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4787
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265187"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            647..1642
                     /locus_tag="VGB68_10055"
     CDS             647..1642
                     /locus_tag="VGB68_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0049618.1"
                     /translation="MSAVLALPDLTNKPFNYESNYQKISAGSAAFLFEKNEKLDFVIS
                     ESYLKACEFYGNHGVGVQEFVRHISLTIEKNNEAEICPTSILRFIESHYIDDFYLAYA
                     CSLGSETAWQCFISKFSSGIRVFAKLFSGNSTIAGEIADSIWTYLYLPSRNGRKRIST
                     YDGRIPLTNWLRVVVSNRLANQRELKSNRIEGLDDIAESSVPFQHDKIDSAMRFKRYG
                     VFVSSSLEKACRELTNQECLLILLRYEKNLELKYISRLFNVHQSTVMRRIERICGKIR
                     RKVRINLATEYKLNEFAVEECIADLLENPNYSIIGKIRESRDETVFRTVNLPRRH"
     gene            2003..2812
                     /locus_tag="VGB68_10060"
     CDS             2003..2812
                     /locus_tag="VGB68_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049619.1"
                     /translation="MKHLVAVVLLVLMVASISTTTFSRNVSFAFPDEAAMEMQPALMS
                     TVGVITIYDVDSRQEVNFLDAVIKGGAFHYPYPGFTNERILQPLPTTADKGGFYISIG
                     RYLDQESAFRVEQTKNAAIKSAVAARREPVSYTVRLVEHLLADWGWEKGRTANIMALT
                     PSSDSRKFKPDVFRQKLSSLAFFKIGYTGQVGVLDFFGKDKTVEQIRQEMSKREWLTG
                     ASIYETEDKELIVYSEYFSTPKTAAGQKLTSSDGKLSGYQAGRVVQNYMSR"
     gene            2841..3809
                     /locus_tag="VGB68_10065"
     CDS             2841..3809
                     /locus_tag="VGB68_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049620.1"
                     /translation="MIRNFVFCITLLALLLSAGGTVSAFGGNDKTPNLTDRLKKKSVR
                     KADAKNKAAKREDVKSKDVKLPGRVYAQVIVYDILPGSETAFEAVIQTSSRNALKSAD
                     FINERVMKNLDELASQYATYAKFATRKAAEEFVQTRLNAVRNLCTRAPETHLAEMTSS
                     YYGKDGYTKNPTGKEFGSGQVGQVAHLGLFIPIPKYREIYDSTLEEVKSTIQKRNSAG
                     YYGEDLLVEADLISPEIQTPFTPRAAEPSQMSINYGEYDTFENAEDSYLARNERRNDP
                     RIAAMQRNFFSALQVPTRFYIFKVIANIDGAQQTTVKTAMLSKPAK"
     gene            3995..>4787
                     /locus_tag="VGB68_10070"
     CDS             3995..>4787
                     /locus_tag="VGB68_10070"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEY0049621.1"
                     /translation="MVAVVSCGIFSPNLKSGGQIYEALFEDPSQPNFTFRKHERLVSK
                     EELQAASGLSYREAKKMDRFSLLAMAAARTALDNADLNEDEYRNCGIITGNMVGGWSF
                     TEPQLRALHTVGLSGVSPYLASAWFPAAPQGQVTIHLGLKGFAKTITTDRSAGAQAIE
                     WALERLKNSRKKEFMLAGGVEAPITPFVEAALTQAGMATETVVEGAAYLLLTSMKAAD
                     AAGETKLGEYTTFPLRYGKGFPSIQIGKALLTVMERGKNLPFPEAV"
BASE COUNT         1340 a         1111 c         1185 g         1151 t
ORIGIN      
        1 taattttcta ttcttttgtt tagacatctg aaaactattt ttttattcag acatctgaaa
       61 acaggcgatt gtgattatcc gagaaaactc gattatctaa atggtgtttt tatgtcaggc
      121 gattcccaca gattgactat aaataaaaca gaagccgtag tgtgcgtctg ccgaaagacg
      181 gcgcgaaagg ttcttcgggt cagtcaaaaa aatcgccgcg cgacgagaaa gaaaagttta
      241 tacaaaaaaa gccgcttatc cgttttcggg atgaccggct ttttttgttg ttccgcgtaa
      301 cgtaatcgga agtttgattt ttgcaggtta aatcaaaagg gaaaggctcg aaaccggtga
      361 cgccaatcac cggcgcgggc tgatttgatt tagccgtgag ccggtaatga ttcgacagat
      421 gcctctttca caagccgcat cccaaatcat tccgtttcct gaaatcaacc cgacaattta
      481 atagaaccct ttgggtcgct gccggcgacg ccgtttttca ataacggcgt tttcgtccgg
      541 ctggtcagtc acacgtgtct gcgcgttcgg aaactcttgc gaaccgcgaa aggtaaatat
      601 gtcaaaagag gaaatagaaa cagtcgccac aggagccgtc agggaaatgt cagcagtgtt
      661 ggctttgcct gatttgacga ataaaccatt caattacgaa agtaattacc aaaaaataag
      721 cgccggaagc gccgccttcc tgtttgaaaa gaatgaaaag ctcgattttg tgatttccga
      781 atcatacctg aaagcgtgcg aattttacgg caatcacgga gtcggcgtgc aggaattcgt
      841 caggcatatt tctttaacta tcgagaaaaa taacgaagcc gaaatatgtc cgaccagtat
      901 cctgcgattt atcgaaagtc attatatcga tgacttctat ttggcttacg cctgctcgct
      961 cggcagcgag acggcgtggc aatgttttat cagtaagttt tcttccggta taagagtttt
     1021 cgccaagctc ttttccggaa attccacgat tgccggagaa atcgccgatt caatctggac
     1081 ttacctttat ctgccctcgc gcaacggtcg caagcgcatt tcgacctacg acggcagaat
     1141 tccgttgacc aactggctgc gggtcgtcgt ctccaatcgt ctggcgaacc agcgtgaatt
     1201 aaaaagcaac cggattgaag gcttggacga cattgccgaa tcttcggtcc cgtttcagca
     1261 cgacaaaatc gactcggcga tgagattcaa acgatacgga gttttcgttt caagctcgct
     1321 tgagaaagcc tgccgcgaat tgaccaatca ggaatgcttg ctgattttgc tccgttacga
     1381 aaaaaatctg gagcttaaat atatttcccg cttgttcaat gttcaccaat cgaccgtaat
     1441 gcggcggatt gaaagaatct gcggcaagat tcgccggaag gtcaggatta atctggcgac
     1501 cgaatataaa ttaaacgaat ttgcggtcga agagtgtatt gccgatttgc tggaaaatcc
     1561 caattactcg atcatcggca aaatcagaga atccagagat gaaaccgttt tccggacggt
     1621 aaatttgccg cgccggcatt gataaaaaac aaaattttaa aagcggaaaa ggcaggcgct
     1681 gaaatcgctt cggattgttc tttgacaagt tacgattctg tttggctgcg cccggcgcgc
     1741 gcggggacaa acgctctgat ttatctcccg aaatttagaa aaactttacg caatttctga
     1801 aagctgaact gtatctacta atagaggacc cgaatccaaa attctgcaga aggcgaccag
     1861 ttctattgtc gctaagggtt cttgttccaa agctttgtct ataacgggaa cttcagttgc
     1921 cgcatcggaa attggagccg gtttaccaaa gctttccatt caagaaagtc taaaaaataa
     1981 ggagctaaat atgaggttag caatgaagca cctagtagcg gtggtgttgc ttgtcttgat
     2041 ggttgcctcc atcagcacaa caacattcag tagaaacgtt tcgttcgcat ttccggacga
     2101 agcagcgatg gaaatgcagc ctgccttgat gtcgacagtc ggcgtaatca ccatttacga
     2161 cgttgattcg cgccaggagg taaattttct ggatgcagtc attaaaggcg gagccttcca
     2221 ttatccgtat cccggcttta ccaatgaacg catcctgcag cctctgccga ctacggcgga
     2281 taagggcggg ttctatatca gtatcgggcg ttatcttgac caggagtcag cttttcgcgt
     2341 ggaacaaact aaaaatgcgg caatcaagtc tgcggttgcc gccagacgcg agccggtcag
     2401 ttatacggtt cgcttagttg aacacttatt ggctgactgg ggatgggaga aaggcagaac
     2461 ggcaaatatt atggcgctta ctccctcttc cgactcaaga aaatttaagc ccgatgtttt
     2521 caggcaaaaa ctcagttctt tagccttttt caaaatcggc tataccggac aggtcggcgt
     2581 gctggacttc ttcgggaagg acaaaaccgt cgaacagatt cggcaggaaa tgagtaaacg
     2641 tgaatggctg acgggcgctt ccatttacga aaccgaagac aaggagctga ttgtctatag
     2701 cgaatacttc agcacgccga aaacggcggc tggacaaaag ttgaccagca gcgacggtaa
     2761 gttgtctggt tatcaggctg gtcgtgtagt gcaaaactat atgtctcgct gaactaagaa
     2821 aataggaggt aaagagaaca atgattagaa actttgtgtt ctgcattaca ctgctcgcgc
     2881 tgctgctttc ggcaggagga actgtttcgg catttggtgg aaatgacaaa actccgaatc
     2941 tgacagaccg cctgaagaaa aaatcagtcc gaaaagcaga tgccaagaat aaagctgcta
     3001 aaagggaaga tgtcaaatca aaagacgtaa aactgccggg acgagtttat gcccaggtga
     3061 ttgtctatga catcctgccc ggaagcgaaa ccgcatttga agccgtcatt caaacgtcta
     3121 gccgcaatgc tcttaaatct gcggatttca ttaacgaaag agtaatgaaa aaccttgacg
     3181 aactggcttc gcaatatgca acttacgcta aattcgccac gcgtaaggca gccgaggaat
     3241 tcgtccagac tcgtttaaac gccgtgagaa acctgtgcac tcgtgcgccg gaaacacatc
     3301 tggctgaaat gacgagctcc tactacggga aagacggata caccaaaaat ccgaccggaa
     3361 aggaatttgg ttccggacag gtcggacagg tcgcccatct cgggcttttc attccaatcc
     3421 cgaaataccg ggagatttac gattccactc tcgaagaagt gaagtcaact attcaaaaga
     3481 ggaattccgc cggttattac ggtgaagact tattggttga agccgatttg atttcgccgg
     3541 aaatccagac tccgttcacg ccgcgcgcgg cggagccatc ccagatgtca attaactacg
     3601 gagaatatga cacgtttgaa aacgcggagg attcttatct ggcgcgcaac gaacggcgca
     3661 acgatccgag gattgccgct atgcaaagga actttttttc agcacttcaa gttccgacgc
     3721 gtttctatat cttcaaagtt attgcaaaca tcgacggcgc tcaacaaact actgtgaaaa
     3781 cggcaatgct ttccaagcca gccaagtaac gcagcttttt gggggtgtct gagtaaagtg
     3841 tctgatttca taaattcagg cttcctcttt tgtgatttca gacactttca tcggcgaaaa
     3901 aattctaaag gtaaaaaata tgctcgatac atttgcacgg gaatccattc aatcagaaac
     3961 gcgcttacct gaaactgacg ccgaagcaaa aaatgtggtc gcggtggttt cctgcggcat
     4021 cttttcacct aatctgaaat ccggcgggca gatttacgaa gcattgtttg aagacccgtc
     4081 tcagccgaat ttcactttcc gcaaacacga gcggctcgtc tcaaaagaag agcttcaagc
     4141 ggcgtcagga ctgagctacc gcgaagcaaa gaaaatggac aggttttcgc tgctggcgat
     4201 ggcggcggcg aggacggctc tggacaacgc ggatttgaac gaagacgaat accgaaactg
     4261 cggaattatt acgggaaata tggtcggcgg ctggtcgttt accgagccgc agctgcgcgc
     4321 tctgcacacc gtcggacttt ccggcgtcag cccgtatctg gcgtcagcct ggtttcccgc
     4381 cgctccgcag gggcaggtaa ccatccatct cggattgaaa ggatttgcca agacgattac
     4441 gaccgaccgc agcgcgggcg cacaggcaat cgaatgggcg ctcgaacggc tgaaaaacag
     4501 ccgcaaaaaa gaatttatgc tggctggcgg cgtcgaagcg ccaatcacgc cgtttgtcga
     4561 agcggcttta actcaagccg gaatggcgac cgaaacggta gtcgaaggag ccgcatacct
     4621 tttgctgaca tcgatgaaag ccgcagatgc ggcgggcgaa acgaagctcg gcgaatatac
     4681 gacttttcca ttgcggtacg gaaaaggctt tccctcgata cagataggaa aagccctgct
     4741 gacagtgatg gaaagaggaa aaaatctgcc gtttcccgaa gcggtga
//
LOCUS       DASWNE010000509         6800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_613526, whole genome shotgun sequence.
ACCESSION   DASWNE010000509 DASWNE010000000
VERSION     DASWNE010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6800
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613526"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            507..1295
                     /gene="larB"
                     /locus_tag="VGB68_10075"
     CDS             507..1295
                     /gene="larB"
                     /locus_tag="VGB68_10075"
                     /inference="COORDINATES: protein motif:HMM:NF033503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HEY0049622.1"
                     /translation="MNQKEIEEILLSHAKGETPLGEAAERLKNLSYENIGYAKVDHAR
                     AERQGFPEVIFGQGKTRAQVVGIFEKLAARSPNVLITRTDADTFGEIRNVFTEAEWHE
                     AAKMIRIRRDKTELGAGEIAVVTAGTSDIPVAEEAALTAETMGNRVKRIWDAGVAGIH
                     RILSEREVLQNARVVVVAAGMEGALPSVVGGLVKVPVIAVPTSIGYGASFGGIAALLG
                     MLNSCASNVTVVNIDNGFGAGFVASLINRKFQENETAADADKRG"
     gene            complement(1529..2953)
                     /locus_tag="VGB68_10080"
     CDS             complement(1529..2953)
                     /locus_tag="VGB68_10080"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0049623.1"
                     /translation="MKSVLSTILLTLVFALTAAAQEYLPELVKRIKPSAVAIETFDAR
                     GAVISRGSGFFISSDKVVTNRHVIEKSARVEVHLINGKKYPVKGVLAIDGEGDLAILQ
                     VDIPQTIAAPLQIEKTTPQEGESIVVIGNPFGLEGSVSNGIVSAVREIPGYGKIIQIT
                     APISPGSSGSPVVNMRGQVIGVATLQAAEGQSLNFAVPSERITQLKLGELQTFAALSA
                     ETQKNKRAAAQSLYSQGLALISRDDYAKAVAYFEKASEIDPNYAEAWYQAGFCYGTLG
                     KHADALKASRRAAALRPEWAETFVNVGASSFALGQFKDAVEAYRQATRLDPYNADAQF
                     ALGLSFNKLNRTDEEILAYKRVVAQKSDHSGALERLGLAYFKLKRYAEAAQAFENLKT
                     YKPEAKTYNYLGESLFELGKTEESIEAFNNAVNLNPDFEKARYNLGKAYLKSGDRDSA
                     GIQYEILRNARSDWADRLLILINP"
     gene            complement(3220..4509)
                     /locus_tag="VGB68_10085"
     CDS             complement(3220..4509)
                     /locus_tag="VGB68_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049624.1"
                     /translation="MQIRYFLPLFLVTLLFSVSAFAESSSKAKELARQAASENQTEAV
                     SAINQLRALRQEGLDALFETYAADIRRFGETGTATDEWKRVAFAIDGVAMQKDAYASR
                     LFWFTDLQQAKLTAKAQNKPILSLRLLGNLNEEFSCANSRFFRAVLYSNAEISKFLRE
                     NYVLHWKSVRPAPKVTIDFGDGRKIERTLTGNSIHYLLDDSGAIIDALPGLYSPQGFL
                     KYIAQAEKVNKIINGASAADKEKAMLRFRRLMFNDILAKRNKAVAASKVKLTEPKNGT
                     SAIDVASRAVTKMAVTDEISILRGISDDFSRYEPQINFDDWNKLAKIYTGGTKIDAES
                     LAFIRRQNAKIGLSETQFTALVEKLEKYIALDTARNDFLFHPKLYEWLNQQSAGDVEA
                     FNTRVYAEIFKTPDSDKWLGMYAPDVYTALDGGGIIK"
     gene            complement(4731..5606)
                     /locus_tag="VGB68_10090"
     CDS             complement(4731..5606)
                     /locus_tag="VGB68_10090"
                     /inference="COORDINATES: protein motif:HMM:NF014646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HEY0049625.1"
                     /translation="MLELNDLTTKEVARLCRVSDATVKRWEEAGLLKSERTSGGHRRF
                     RAEEVARFQRRQNIGSEQPRGVESARAALRRRRIKKSYSDSLLFHSLLAGREDEAANI
                     LISAHLHDKPLAQIFDESVCPAMRRVGELWFDGEINVAQEHLAARTAHNALYKLRESI
                     PVGGINGRLAFCCAIEGDFHELPTHLAQMALENEGWEAINFGANTPLYSLTDEVLYHS
                     PEIVCIAAAIINDYERLARDYKELRARIAKLKIPIVLGGRAFADKRIRARFPAELYAE
                     NFTQFAEFVKSFPVK"
     gene            6007..6477
                     /gene="rpiB"
                     /locus_tag="VGB68_10095"
     CDS             6007..6477
                     /gene="rpiB"
                     /locus_tag="VGB68_10095"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEY0049626.1"
                     /translation="MTKPKIAIGADHAGFEEKEKIKKVLDEIGVEYEDFGTTSLDSVD
                     YPDFGAKVGRAVRDGLVRQGIVVCGSGIGIAIAANKIHGVRAAQAWNEETARLARRHN
                     DANVLSIGARVLPEDEIQKIVRAWFDADFEGGRHAKRVEKISELENEENRALAR"
     gene            6486..6767
                     /locus_tag="VGB68_10100"
     CDS             6486..6767
                     /locus_tag="VGB68_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049627.1"
                     /translation="MWDLGEFRLIAYFFSLLGVIALALAAGDKSHLKTGAFYIKKAAW
                     AVLILVSFYWVISIPHAGLYHEYPKTVDYPTSISADEADRYIKENHSRI"
BASE COUNT         1693 a         1685 c         1763 g         1659 t
ORIGIN      
        1 gaccgttgcg gacgtgcccg acattatcaa attcacctgc gagaccgcgc cgctttccgc
       61 caaaagctgc aaggtgcggt aagcgtaaat cgtgcccgat gcgcccgtta gggcgactgt
      121 caattccata tagattttta ctctacactt tttttcggga atgtcacaaa ttaaaaaaaa
      181 ctttagaaaa gatgaaatga atctccgccc gaatacgctt taacaggtag aggcgctttg
      241 gcatcggaat tgctcaaagg cgcgggcaga aagaatttct cgaaaaggag aacgaaaatg
      301 atacaaaaat ttatccagag cgatttatta ttgaacctcg tcggcaatga aattgtcggc
      361 tggtttttga tgaagatggt ctcgtcttat aaatacgtcg gtttattcgt ttaattatta
      421 agctattatt tgtttttata cgggggcggc tgaaatgaaa atttcgccgc tttttacttt
      481 tcgccgcgaa gctttaaact aattttatga atcagaagga aatcgaggag attctgctct
      541 cgcacgcgaa gggcgaaacg ccgctcggag aagccgccga gcgactgaaa aatctgtcgt
      601 acgaaaacat cggctacgcg aaagtcgacc acgcgcgcgc cgagcgacag ggcttcccgg
      661 aagtcatttt cgggcaggga aaaacgcgcg ctcaggtcgt agggattttt gaaaagctcg
      721 cggcgcgctc gccgaacgtt ttgattacgc gcacggacgc cgatactttc ggcgaaatcc
      781 gcaacgtttt taccgaagcc gaatggcacg aagcggcaaa gatgattcgc atccgccgcg
      841 acaaaaccga gctcggcgcg ggcgaaatcg ccgtcgtcac cgccgggacg agcgatattc
      901 ccgtcgccga agaagccgct ctcacagcgg aaacgatggg caaccgggtc aaaagaatct
      961 gggacgccgg agttgccgga atccaccgga ttttgtcgga aagagaggtt ttgcaaaacg
     1021 cgcgcgtggt ggtcgtcgcg gcgggaatgg aaggcgcgct gccgtcggtc gtcggcggtc
     1081 tggtcaaagt tccggtcatc gccgtcccga ccagcatcgg ttacggcgcg tcgttcggcg
     1141 gcatcgccgc gctgctcgga atgctcaatt cctgcgcttc aaacgtgacg gtcgtcaata
     1201 tcgacaacgg cttcggcgcg ggctttgtcg ccagcctgat aaaccgaaaa ttccaggaga
     1261 atgaaaccgc agccgacgca gataaacgcg gatagaaaaa taaaaatcgt atttatctgt
     1321 attaatctca tatgaatttg ccggttttag gtaagaaaaa ggcgtttact tgttagtaaa
     1381 ttgaatttac taacaagtaa acgcctttta ctaacaagta aattcaattt actaactagc
     1441 aaacgtgatt tactaactag caaacgtgat ttactaacta tcaaatggtg ttttccaact
     1501 ataaaattgt aatcgagcga ttggaaaatt aaggattgat taaaatgagc agacggtcag
     1561 cccaatccga acgcgcgttg cgcaggattt cgtattgaat accggcggaa tcgcggtcgc
     1621 cggatttcag ataagctttt ccgagattgt agcgggcttt ttcgaagtcg ggattcagat
     1681 tcacggcatt gttaaaagct tcgatgcttt cctcggtttt gccgagctca aacaagcttt
     1741 cgcccagata attgtaggtt ttcgcctcgg gtttgtaggt tttcagattt tcaaacgcct
     1801 gcgccgcttc cgcgtagcgc tttaacttaa aataagcgag tccgagtcgt tcgagcgcgc
     1861 ccgagtggtc ggatttctgc gccacgacgc gtttatacgc cagaatttct tcgtccgtgc
     1921 gatttaattt attaaaactg agcccgagcg caaattgcgc gtcggcgttg tacgggtcga
     1981 gcctggttgc ctgtcgataa gcctcgaccg catctttaaa ctgtccgagc gcgaagctcg
     2041 acgcgccgac gttgacaaaa gtttccgccc attccggtcg caaagcggcg gcgcggcgcg
     2101 aagccttcag ggcgtcggcg tgtttgccca gcgttccgta acaaaatccg gcttgatacc
     2161 acgcttcggc gtaattcggg tctatctccg acgctttttc gaaataagcg acggcttttg
     2221 cgtaatcgtc gcgcgaaatc agcgccagac cctgcgaata gagagattgc gcggcggcgc
     2281 gtttgttttt ctgcgtttcg gcgctgagcg cggcgaaggt ctgcaattcg cctaatttca
     2341 actgcgtgat tctttccgaa ggcacggcaa aattcagact ctgaccttcg gcggcttgca
     2401 gcgtcgccac gccgataacc tgcccgcgca tattgacgac cggcgagcct gaagagccgg
     2461 gcgaaatcgg cgcggtaatc tggatgattt tcccgtaacc gggaatttcg cgcaccgccg
     2521 agacgatgcc gttcgagacc gagccttcca gcccgaacgg attgccgata acgacaatcg
     2581 actcgccttc ctgcggcgtg gttttttcaa tctgaagcgg cgcggcgatt gtttgcggaa
     2641 tgtcaacctg caaaatcgcc aaatcgcctt cgccgtcgat tgccagaacg cccttgaccg
     2701 gatatttttt gccgttgata agatgcactt cgacgcgggc tgatttttcg atgacgtggc
     2761 gattggtcac gactttatcg ctggaaatga aaaagccgct gccgcgcgaa atgaccgcgc
     2821 cgcgcgcgtc gaacgtttcg atggcgacgg cggacggctt gatgcgcttg accagctccg
     2881 gcagatattc ctgcgcggcg gcggtcaatg caaaaacgag cgtcaataaa attgtcgaaa
     2941 ggacggattt cattaatcta cggcgaaatt ataaagcgtt tatttgaatc ggacaaataa
     3001 gatagatgta tccggcgggc gaaaccgttg caataattgt ccgggcgcgg ctgtcgatgg
     3061 aaggattaac cgcgaaagaa cacgaaaaag cgcgaaagaa gaattaaccg aagatagcgg
     3121 aaataaacag ggatgattta aataaaaatc atttttctta tccctatcta ttcctgttat
     3181 ccctatattt tccttttcgc gcctttccgg gactaaggct tatttaataa tgccgccgcc
     3241 gtcgagcgcc gtgtaaacgt cgggcgcata cattccgagc cacttgtcgg aatccggcgt
     3301 tttaaagatt tcggcgtaaa cgcgagtgtt gaacgcctcg acatcgccgg cgctttgctg
     3361 attcagccat tcgtacagct tcgggtgaaa aagaaaatcg ttgcgcgccg tgtcgagcgc
     3421 gatatatttt tccagctttt cgacaagagc tgtaaattgg gtttcgctca agccgatttt
     3481 ggcattttga cgacgaataa aggcgaggct ttcggcgtcg attttcgttc cgcccgtgta
     3541 aattttggcg agcttgttcc aatcgtcgaa attgatttgc ggttcgtagc gcgaaaaatc
     3601 atcggaaatg ccgcgcaaaa tcgaaatttc atccgtcacc gccattttgg tcaccgctcg
     3661 cgaggcgacg tcgattgcag acgtgccgtt tttcggctcg gttaatttta cttttgacgc
     3721 ggcgaccgct ttattgcgct tcgctaaaat gtcgttgaac atcaggcggc gaaatctgag
     3781 catcgccttt tccttgtcgg cggcagacgc gccgttgatg attttattga ctttttcggc
     3841 ttgcgcgatg tatttcaaaa atccctgcgg gctgtataga ccgggcaaag cgtcgatgat
     3901 tgcgccgctg tcgtccagaa gataatgaat gctgttgccg gtcaaagtgc gctcgatttt
     3961 gcgaccgtcg ccgaaatcaa tcgtcacctt cggagcggga cgcaccgatt tccagtgcag
     4021 aacgtaattt tcgcgcagaa attttgaaat ttccgcgttc gaataaagca cggcgcgaaa
     4081 gaaacgactg tttgcgcagc tgaattcttc gtttaaattt cccagcaggc gcaggctcaa
     4141 gataggtttg ttttgcgctt ttgccgtcag cttcgcctgc tgcaaatccg taaaccagaa
     4201 caatcgcgaa gcgtacgcgt ccttttgcat cgcgacgccg tcaatcgcaa aagcgacgcg
     4261 tttccattca tcggtcgctg tgcccgtttc gccgaaacgc ctgatgtcgg cggcatatgt
     4321 ttcaaacagc gcgtccaagc cttcctggcg cagggcgcga agctggttga tggcggaaac
     4381 cgcttcagtc tggttttcag acgcggcttg tcgggctaat tctttggctt tacttgacga
     4441 ttcggcgaaa gcggaaacgg aaaaaagaag cgtaacgaga aacaggggca gaaaataccg
     4501 gatttgcata gacctttctc caaagacgga tgatttcggg aagagctcac atctttttag
     4561 agatttgagt ctcaatttgg ttgcctgcgg cgattaaaat cgctctgcct tcttcgcgtc
     4621 ggaaagcttt cgcaggaatc gctgagggat tttctcgccc gaaattaaaa gaatagccgt
     4681 aaattcatag aatataagag cttacggcta tttgccgggg tgaaatttaa ctatttaacc
     4741 ggaaagcttt taacgaattc ggcaaactgt gtaaaatttt cggcgtataa ttcggcggga
     4801 aaacgcgctc tgatccgttt gtcggcaaaa gctctgccgc ccaaaacgat tggaattttt
     4861 aatttcgcga tgcgcgcgcg aagttccttg taatcgcgcg ccagccgctc gtaatcgttg
     4921 ataatcgccg ccgcgatgca gacgatttcc ggcgagtgat acaaaacttc gtcggtcaga
     4981 gagtaaagcg gcgtgttagc gccgaaattt atcgcttccc agccttcgtt ttccagcgcc
     5041 atctgtgcca gatgcgtcgg aagctcatga aaatcgcctt caatcgcgca gcaaaaagcg
     5101 agcctgccgt ttattccgcc gaccggaatg gattctctga gcttgtaaag cgcattatgc
     5161 gcggttcgcg cggcgagatg ttcctgcgcc acgtttatct cgccgtcgaa ccaaagctcg
     5221 ccgacgcgcc gcatcgcggg acagaccgat tcgtcaaaaa tctgcgccag cggcttatcg
     5281 tgcagatgag cgctgatcag aatatttgcg gcttcgtcct cgcgcccggc aagcagcgaa
     5341 tgaaacagca gagaatccga atagcttttt tttatgcgac gacgacgcag cgccgctctg
     5401 gctgattcga cgccgcgcgg ctgctccgag ccgatgtttt gccggcgctg aaaacgagcg
     5461 acttcctcgg cgcgaaaacg acggtgaccg ccgctcgtgc gctcggattt cagcagaccg
     5521 gcttcttccc agcgtttgac ggtcgcatcg ctgacgcggc acagccgggc tacttctttc
     5581 gtcgttaaat cgttcagttc cagcattttt ctttctcagc catttttagt ttatacaaag
     5641 cgcacgaatc gcacaaataa aatttaataa cttcagaaat tttctgaaac gccaataaga
     5701 aaataaaaaa tgcttgacaa ttgaccattt cgcttataag ataaaaccgc tcaaaccgct
     5761 caaaatcgct tttcatcaaa gcgagcaaga gaaggttaag gaaaagaatc gtcatcagcc
     5821 ggaaggggaa gccggcggcg attcttttcc gtttttaccg cgaaaataat ttgcggcgcg
     5881 cttatcttcg ccggttcgcc ctttgtcatt tgtccttaat cctttgcaat tgtttctaaa
     5941 aatagagatt tcggctaaaa tcaaaaaaca actgacgcag gacaaatgac gaaggacgaa
     6001 tcgtaaatga ctaaaccgaa aatcgccatc ggcgcagacc acgccggatt tgaagaaaag
     6061 gaaaaaatca aaaaagtttt agacgaaatc ggcgtcgaat acgaagattt cggcacgacc
     6121 tcgctcgatt cggtcgatta cccggatttc ggcgcaaagg tcggcagagc cgtccgcgac
     6181 ggcttagtcc ggcagggaat cgtcgtctgc ggctccggca tcggcatcgc tatcgcggca
     6241 aataaaatcc acggcgtccg cgccgcccaa gcctggaacg aagaaaccgc ccgcctcgcc
     6301 cgccggcata acgacgccaa cgtgctttcc atcggcgcgc gcgttctgcc ggaagacgag
     6361 attcagaaaa tcgtccgcgc ctggttcgac gcggattttg aaggcgggcg gcacgctaaa
     6421 agagtcgaga aaatcagcga gctggaaaac gaagaaaatc gcgctttagc gagataattt
     6481 agtttatgtg ggatttaggc gaattcagat tgattgcata tttctttagt ctgctcggcg
     6541 taattgctct ggctttggcg gcaggagata aatcacactt gaaaaccggc gcgttctata
     6601 taaaaaaagc tgcgtgggcg gtattgattc tggtttcgtt ttactgggta atttcgatac
     6661 cccacgccgg tctttatcac gaatatccga aaactgtgga ttatccgact tcgataagcg
     6721 cggatgaagc ggatagatac atcaaagaaa atcattcccg catttaaggc ttgcaaaatg
     6781 aattaaggga aactaaagag
//
LOCUS       DASWNE010000510         8594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_653033, whole genome shotgun sequence.
ACCESSION   DASWNE010000510 DASWNE010000000
VERSION     DASWNE010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8594
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653033"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="VGB68_10105"
     CDS             complement(<1..779)
                     /locus_tag="VGB68_10105"
                     /inference="COORDINATES: protein motif:HMM:NF016142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HEY0049628.1"
                     /translation="MNIKFNEEVAAALRDKQPVVALESTVIAHGLPFPQNLETARRLE
                     QIVRENGATPATVAVFDGEFCAGLNEAQIERLADNKNIRKISRRDLPIAVAKKLNCAT
                     TVATTTFVAHRAGIKVFATGGIGGVHRGFAADISADLPELAQTPITIVCSGAKIVLDL
                     PATREWLETYGVPILGWRCDELPAFYSSKSGLSVDERIETAQEAARIAQSRDELNLQT
                     AVLVAVPVPPEFEINSDELEKILSGALRAAAEKNVRGKEITP"
     gene            complement(904..1752)
                     /gene="lepB"
                     /locus_tag="VGB68_10110"
     CDS             complement(904..1752)
                     /gene="lepB"
                     /locus_tag="VGB68_10110"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY0049629.1"
                     /translation="MSKTETEVTETAPKPAGPPKSAFREYLESFVVTLIMALFGMTFV
                     IQAVTVPTGSMQNTILVGDYLLVNKFIFAPGGRPVPLLPQREVQRGDIIVFKFPGYQD
                     KRLAPALEIQGAGTPYTTNFVKRVIGMPGDTLEFRNNQVYINGELLPEHRIVADTPMT
                     GDGDDNDQAPLAVKSEESREDNERYSVYYSERTMQAAEKGRLPVSGRYGVPGGQIKVP
                     EDSYFVLGDSRDRSLDSRFWGFVHRDLIIGRAMFVYWSCDRTDSSGTFGCITNPRLDR
                     IGKFIR"
     gene            complement(1800..2531)
                     /gene="rnc"
                     /locus_tag="VGB68_10115"
     CDS             complement(1800..2531)
                     /gene="rnc"
                     /locus_tag="VGB68_10115"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEY0049630.1"
                     /translation="MSQNLARLEQLLGYKFQNLELLQRALTHRSWAYENAPFADNGEA
                     ARSLQNESLELVGDAVLGLAVVEQLYNKHSAATEGDLTLMKHRLVSTGTLATAAQRIN
                     LGEFLRVGKGEEKTGGRRKQALLADTFEAVLGAIFFDSGYISARAFVQRVLADEIRNA
                     TPTSSIDYKTLLQETLQADKREAPSYNVIKTSGPPHNRVFHVEAAWDGGAVRGKGTSI
                     KQAEMMAANLALKQLKKERKTENQT"
     gene            complement(2637..3890)
                     /locus_tag="VGB68_10120"
     CDS             complement(2637..3890)
                     /locus_tag="VGB68_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049631.1"
                     /translation="MNTVFKVLALGMMLMAFAVAPAFAQQEECEALYKKFTDNYKGTL
                     AQRKMAVEGGEQYIAKCAALEGSADIIKYLNEKGLPPLRKKIDFEETASAFDAAVKDA
                     KTVNADVAFTSGKRLLANHDPNLVDVMIVLASVGFDQATAATPVNTYNADAVNFAKQS
                     IQKIEGGATSKQFGAWGYTYKTKENALAWMNYTIGYITYFNQKNKKEALPYLYRATKY
                     ETGGKDNPKNNAIVYQAIGDYYKDEYNRLDDERVKLATEAKDKTPEEAKPILDKAKEV
                     LLLQKAYADRMIDAYARARAFSTADKAYQDSLYNNLKVLYAFRHDNKPTTPLDAYISS
                     VTGKPMPDPTSTVTPVVDTTTTTTATTPTTSSLTSTPTSTTTNNARTTTDTTVSKQAT
                     TTTTKAKTTTKTPAKTPAPKKKGTR"
     gene            complement(4246..5796)
                     /locus_tag="VGB68_10125"
     CDS             complement(4246..5796)
                     /locus_tag="VGB68_10125"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY0049632.1"
                     /translation="MFFSAEKLYFNRVFGLFLILFLAALSADVQAQARKFAPKQDKNK
                     VEKQDKNSRNPTRAESAREAENKKQTMERERADQRRRDEARQAELVRQRAAQEERRRR
                     EQIAREADARRRAFERNLKTETLENISNDNPEGEDLEIRRAAVNALGGHAGTVVVLEP
                     QTGRILSIVNQDWAIRKGFKPCSTIKLVTGVAGLNERIIGSTGEIRTRPFPMDLNDAL
                     AFSNNAYFQRVGVTGVGNEKMISYARMLGLGEPTGINADGETAGKLPFGNSNPRIYSH
                     GDDFEVTPLQLAVMVSAISNGGRLVVPQIARTKIEKANFRGQMRRQINFSAQNLEGVL
                     PGMIGAVTYGTARRSNGAPLNVAGKTGSCIGQGSWVGLFASVAPAVNPQIAVVVITRG
                     QSERGKYASAIAGKIYQAVSYRFKDERREFVAKTPLELVKPQPKIDAQTSAQLDDEEG
                     EDSDEGDASGVKIKKQETPPQSKKPELKQQAAKNTFAPVVIKYKRDGAEEPDGSLPTR
                     PRIVKTNK"
     gene            6026..7510
                     /locus_tag="VGB68_10130"
     CDS             6026..7510
                     /locus_tag="VGB68_10130"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0049633.1"
                     /translation="MNFFNTFRGRLLIVLAFLLIATLGVQYFINLQTERRNKRYRERQ
                     EQALVAGIALGFNGMQSRDRLSDIVKQENQRFFDESITNRITDIIVINDKWQVYDSLN
                     PDYQETEDEHGSAIYKNLRDLTDLPPLQDADRLGEDRENFPNAGKEESDTDEAEAHAI
                     PIQTNQGRFYVMVLLKTNRAKAAWNAARPLFYTLSIFSVSTIITIFLVWRFTRPIANL
                     SEAAKRIADGDLTVRVPHDKRSDEMGRLASRFNEMTAELAKKSELEAMLQEAEKSAVV
                     GRLGSAIAHEIRNPLNYINLTLDHLRAKFAPEDAGKRRDFEKLTLQLKSEVARINQQI
                     TDFLSYSRPPKLNLEPTDLKAVVKDSLRIVEAEAEEQNIKINFAENGGAPRVYGDAEY
                     LRSVFNNLFINAVQSMATGGGNLNVSIEPLDAYVKIEVGDTGGGIPEENLSKIFEPYF
                     STKETGTGLGLAIVKRVVDEHRGVIGVESRLHEGTTFTVRLPKA"
     gene            complement(7607..8272)
                     /locus_tag="VGB68_10135"
     CDS             complement(7607..8272)
                     /locus_tag="VGB68_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049634.1"
                     /translation="MKARDLNRLSDHELSAQVRLMNDNATGDEASYGLTASLTAALKA
                     ELDGFDDDLATLEGLEASENAARGAKNNRRAKIIELTRKQMGLIRSTVGNDADKLGKV
                     NLDPLDETRTEAGAPVSVPFALIDLGKLRHTLNFRDAATPDTDKKPGGILGAEIWYKI
                     GGTAPVDNKECQFLALDTATPYVIQFDGADAGKTVYYLLRWQSKNGDKGDWSDVAQAT
                     VNG"
BASE COUNT         1878 a         2270 c         2274 g         2172 t
ORIGIN      
        1 ggcgtaatct ccttgccgcg cacgtttttt tcggcggcgg ctcgaagcgc gccgctcaaa
       61 atcttttcga gctcgtctga attaatttca aattccggcg gaaccggcac ggcgaccaga
      121 accgccgttt gtaaattcaa ttcgtcgcgc gactgcgcaa tgcgcgcggc ttcctgcgcc
      181 gtttcgattc gctcgtcaac cgaaagcccg cttttcgagg aataaaaagc gggaagctcg
      241 tcgcatcgcc agccgagaat cggaacgccg taggtttcca gccattcgcg cgtggcgggc
      301 aaatccagaa cgattttcgc gcccgaacag acgattgtaa tcggagtttg cgccagttcc
      361 ggcaaatccg ccgagatgtc agccgcaaat ccccgatgga cgccgccgat gccgcccgtc
      421 gcaaaaactt tgattccggc gcgatgggcg acgaaagtcg ttgtggcgac ggtcgtcgcg
      481 cagtttagtt ttttggcgac ggcaatcggc aaatcgcgcc gcgagatttt gcgaatgttt
      541 ttattgtcgg cgagtcgttc gatttgagct tcgtttaaac cggcgcagaa ctcgccgtcg
      601 aaaacggcga cggtggctgg cgtcgcgccg ttttcgcgga cgatttgctc aagccgtcga
      661 gcggtttcga gattttgcgg aaagggaagc ccgtgcgcga taaccgtcga ttcgagcgcg
      721 acgaccggct gtttatcccg taacgccgcg gcgacttctt cgttaaattt gatattcatt
      781 attcagagct tgggattcag gattcaggat tggaaatcgg attgaataat gaatcctgaa
      841 tcttgaatcc tgaatcctga atcttaaatc ctgaatcttg aatcctgaat cctgaatcct
      901 gaattatcta ataaactttc cgatgcggtc gaggcgcgga ttcgtgatgc agccgaaagt
      961 tccgctgctg tcggtgcggt cgcacgacca gtaaacgaac atcgcgcgtc cgatgattaa
     1021 atcgcgatga acaaagcccc agaaccggct gtccagactg cggtcgcggc tgtcgccgag
     1081 cacgaaataa ctatcttcgg gaacttttat ttgtccgccc ggaacgccgt atctgccgga
     1141 gacgggcagc ctgccctttt ccgccgcctg catagttctt tccgaataat aaacgctgta
     1201 gcgttcgtta tcctcgcgcg attcctcgct tttgaccgcg agcggagcct ggtcgttgtc
     1261 gtcgccgtcg ccggtcatcg gcgtatcggc gacaatccgg tgttcgggca gcaattcgcc
     1321 gttaatgtaa acctgattgt tgcgaaactc gagtgtatcg cccggcatac cgataacgcg
     1381 cttgacgaaa tttgtcgtat aaggcgttcc cgcgccctga atttccagag ccggagccag
     1441 ccgcttgtcc tgataaccgg gaaacttgaa cacgatgatg tcgccgcgct gaacctcgcg
     1501 ctgcggcaaa agcggcacgg gacgcccgcc cggcgcgaag ataaatttgt taaccagaag
     1561 gtaatcgccg accagaatcg tgttctgcat cgagccggtc ggaaccgtca cggcttgaat
     1621 gacgaacgtc attccgaaaa gcgccataat cagcgttacg acaaacgatt ccaaatattc
     1681 gcggaaagcg cttttcggcg gtccggcggg tttcggggct gtttctgtta cttcggtttc
     1741 tgttttactc attttaaaaa tcgtaaatgt ctcggtttaa ttatacgcaa taaaaaatct
     1801 taagtttgat tttcagtctt tctttctttc tttaattgtt tcagcgccag atttgccgcc
     1861 atcatctcgg cttgctttat cgaagtgcct ttgccgcgca ccgcgccgcc gtcccacgcg
     1921 gcttcgacgt gaaagacgcg attgtgcggc ggtccggaag ttttgataac gttgtaggac
     1981 ggagcttcgc gcttatcagc ctggagcgtt tcctgcaata aagttttata gtcaatcgaa
     2041 gacgtcggcg tcgcgttgcg aatctcgtcg gctaaaactc tctgcacgaa agcccgcgcc
     2101 gagatataac cgctgtcgaa aaaaatcgcg ccgagaacgg cttcgaacgt gtcggcaagc
     2161 agcgcctgct tgcgcctgcc gccggttttt tcctcgcctt tgccgacgcg caggaattcg
     2221 cccagattga ttctctgcgc cgccgtcgcc agcgttccgg tgctgaccag acgatgcttc
     2281 atcagcgtca aatcgccctc ggtcgctgcc gagtgtttat tataaagctg ttcgaccaca
     2341 gctaagccga gaaccgcgtc gccgaccagt tcgagcgatt cgttctgcaa actgcgcgcc
     2401 gcctcgccat tgtcggcaaa aggcgcgttt tcataagccc acgagcgatg cgtcagggcg
     2461 cgctggagca attccaggtt ttgaaatttg taaccgagca gctgttcgag acgggcaaga
     2521 ttttgcgaca tagacgttat tatagaaaaa cgcggcaaaa aagaaagtca gacgccgtaa
     2581 tttagcgtct gacctcacgt taagaagttt ttcaaagact gttcacgctt tgaaggttag
     2641 cgagttcctt tctttttggg cgcgggcgtt ttagccggcg tcttggtcgt ggtttttgct
     2701 tttgtcgtcg tcgttgtcgc ctgtttcgac acggtcgtat ccgtcgtcgt tctggcgttg
     2761 ttcgtcgtcg tgctggtcgg cgtcgaggtc agcgagctgg tcgtcggcgt cgtagcagtt
     2821 gtcgtcgtgg tcgtatcgac caccggcgtt acggtgctgg tcgggtcggg catcggtttt
     2881 cccgtcacgc tcgaaatata cgcgtcgaga ggcgttgtcg gcttattgtc gtgacgaaac
     2941 gcgtaaagaa ctttcaggtt gttgtaaaga gaatcctgat aagccttgtc ggcggtagaa
     3001 aaagcccgcg cgcgcgcgta agcgtcaatc atacggtcgg cgtaagcttt ttgcagtaaa
     3061 agcacttctt tggctttatc gagtatcggc ttggcttctt caggcgtttt atccttcgct
     3121 tcggtggcta atttaacgcg ctcgtcgtca agacggttgt attcgtcctt gtaataatcg
     3181 ccgattgcct gatagacgat ggcattgttt ttgggattat ctttgccgcc ggtttcatac
     3241 ttggtggctc tgtaaagata aggaagcgct tcttttttgt ttttctggtt gaaataggtg
     3301 atatagccga tcgtgtagtt catccacgcc agcgcgtttt ccttcgtctt gtaggtatag
     3361 ccccaagcgc cgaattgttt ggacgtcgcg ccgccttcga ttttctgaat cgactgtttg
     3421 gcaaaattaa ccgcgtcggc attatacgta ttgaccggcg tcgcggcggt agcctggtcg
     3481 aaaccgaccg acgccagcac gatcatcaca tcgaccagat tggggtcgtg attggcgagc
     3541 aggcgcttgc cgctggtaaa cgccacatcc gcgttaacgg ttttcgcgtc tttaacggct
     3601 gcgtcaaaag ccgaagcggt ttcttcgaaa tcgatttttt tcctgagcgg cggaagtcct
     3661 ttttcgttta aatatttgat aatatccgcg ctgccttcga gagcggcgca ttttgcgata
     3721 tattgctcgc cgccttcgac cgccattttt ctctgcgcca gcgttccctt atagttgtcc
     3781 gtaaattttt tatagagggc ttcgcattct tcctgctggg cgaaagccgg agcgacggcg
     3841 aaagccatca gcatcattcc aagcgctaaa actttaaata ctgtattcat tcgatttaat
     3901 cctccaaacg ttgtccgtct ctgcaaaact tctaaacttt gtggcagttc cgtattgcgt
     3961 aatctcagat gtttaccccg aaaaaaaagt tgcaaatctt gtcagggaaa tataaacgca
     4021 attttcctta ttcttattat caagaataac tgttttatat acggaaaaca aaataatcac
     4081 gcaagtttaa tatgcgtgtc aagcgaaaag cgccgaaagg cgaaacgcga agcgcgaaag
     4141 acgaaacgcg aaaggcaaaa ccggcagccg gtaatttcga gctgaaatcg aagattatcc
     4201 gctgccggtt ttgcgccttt ttacttttgc ctttcgcctt ttgtcttatt tattcgtttt
     4261 cacgatgcgc gggcgcgtcg gcagagagcc gtcgggctct tccgcgccgt cacgtttgta
     4321 tttaatcacg accggcgcga aagtgttttt tgcggcttgc tgtttgagtt ccggtttctt
     4381 cgactgcggc ggagtttcct gttttttgat tttgacgccg ctcgcgtcgc cttcgtccga
     4441 gtcttcgcct tcctcgtcgt cgagctgggc ggaagtctgc gcgtcgattt tcggctgcgg
     4501 cttgacgagc tcgagcggag ttttagcgac gaattcgcgg cgctcgtctt taaaacgata
     4561 gcttaccgcc tgataaattt ttccggcgat tgccgaagcg tatttgccgc gctcgctttg
     4621 accgcgcgta atgacgacga cggcgatttg cgggttgacg gcgggcgcga ccgaggcgaa
     4681 aagcccgacc cacgagcctt gcccgatgca cgagccggtt tttccggcga cgttcagcgg
     4741 cgcgccgttc gagcggcgcg ccgttccgta agtgacggct ccaatcattc cgggcagaac
     4801 gccttccaga ttctgcgcgg aaaaattgat ttgacgacgc atctgcccgc gaaaattcgc
     4861 tttttcgatt ttcgtgcgcg caatctgcgg aacgacgagc ctgccgccgt tggaaatcgc
     4921 cgaaaccatc acggcaagct gtagaggcgt cacttcaaaa tcgtcgccgt gcgaataaat
     4981 gcgcgggttg ctgttgccga acggcagctt tccggcggtt tcgccgtcgg cgttaattcc
     5041 ggtcggctcg cccaaaccga gcatccgggc gtaggaaatc attttttcgt tgccgacgcc
     5101 cgtaacgccg acgcgctgaa aataagcgtt gttggaaaac gccagcgcgt cgtttaaatc
     5161 catcggaaac gggcgcgtcc gaatctcgcc agtcgagccg ataattcttt cgttcaaacc
     5221 cgcgacgccg gtgaccagct ttatcgtcga gcacggtttg aaacctttgc gaatcgccca
     5281 atcctgattg acgatggaca gaattcttcc ggtttgcggc tctaaaacga cgaccgttcc
     5341 ggcgtgaccg ccgagcgcgt tgacggcggc gcggcgaatt tccaaatctt cgccttccgg
     5401 attgtcgttc gagatatttt ccagcgtctc cgtcttcaga ttgcgctcga aggcgcggcg
     5461 gcgcgcatcc gcctcgcggg cgatttgctc gcggcgacgg cgttcttcct gcgcggctcg
     5521 ctggcgcacg agttccgcct gccgggcttc gtcgcggcgt ctctggtcgg cgcgctcgcg
     5581 ctccatcgtt tgttttttat tttcggcttc gcgcgccgat tcggctcggg tcgggttcct
     5641 tgaatttttg tcctgttttt cgactttgtt tttatcctgt ttgggcgcga attttctcgc
     5701 ctgcgcctga acgtcggcgg aaagcgcggc aagaaataaa attaaaaaca atccgaagac
     5761 acggttaaaa tacaactttt cggcggaaaa aaacatcgtc tttacctctt cctggttttt
     5821 tgaatttgtt tgggtgtttt gaggagagtt taaagcaaat gaatgagcga cccgtagatt
     5881 aggtgctcga aaagtgtttg ctctaagcca aacaagacta taacattcaa gacgaaagaa
     5941 cggcaaggaa attcggaagg caaaagtaaa aaaaataaaa ggtcggtcta tcgccggcag
     6001 gcaagagcca atcgacaaag ggcatatgaa ttttttcaac acatttcgcg gcagactgct
     6061 tatcgttttg gcgtttctgt tgattgccac gctcggcgtc cagtatttta tcaatttgca
     6121 gaccgaaagg cggaacaagc gttaccgtga aagacaggaa caggcgctgg ttgcgggcat
     6181 cgcgctcggc tttaacggga tgcagtcgcg cgaccgtctg agcgatatcg tcaaacagga
     6241 aaatcagagg ttttttgacg agagcatcac caatcgcatc acggacatta tcgtcatcaa
     6301 cgataaatgg caggtttacg acagcctgaa tcccgattat caggaaaccg aagacgaaca
     6361 cggcagcgcc atttataaaa acctgcggga tttgacggat ttgccgccgc ttcaggatgc
     6421 cgaccggctc ggcgaagacc gggaaaattt tccgaacgcc ggaaaagagg aatcagacac
     6481 cgacgaagcc gaagcgcacg cgattccgat tcagacgaat cagggacgtt tttacgtgat
     6541 ggtgcttttg aagaccaacc gggcgaaagc cgcctggaat gcggcgcgac ctttgtttta
     6601 cacgctctcg attttttccg tctcgaccat tatcacgatt tttctggtct ggcgttttac
     6661 gcgcccgatt gccaatttgt cggaagccgc caagagaatt gccgacggcg atttgacggt
     6721 gcgcgtgccg cacgacaagc gctcggacga gatgggcagg ctggcttcgc gttttaacga
     6781 gatgacggcg gagctggcga agaaaagcga gctcgaagcg atgctgcagg aagcggaaaa
     6841 aagcgcggtc gtcgggcggc tcggctcggc aatcgcgcac gaaattcgca atccgctcaa
     6901 ttacatcaac ctgacgctcg accacctgcg ggcaaagttt gcgccggagg acgcgggcaa
     6961 gcgccgcgat tttgaaaagc tgaccctgca attgaaatcc gaagtcgcgc gcattaacca
     7021 gcaaattacc gattttttaa gttattcgcg cccgccgaaa ctgaacctgg agccgaccga
     7081 cctcaaagcg gtcgtcaaag attcgctcag gattgtcgaa gccgaagccg aggagcagaa
     7141 cataaaaata aatttcgccg aaaacggcgg cgcgccgcga gtttacggcg acgccgaata
     7201 tctgcgctcg gttttcaaca atcttttcat caacgccgtg cagtcgatgg cgaccggcgg
     7261 cggcaatctg aacgtttcca tcgagccgct ggacgcttac gtgaaaatcg aagtcggcga
     7321 tacgggcggc ggcattcccg aagaaaatct ctcgaagatt ttcgagccgt atttttcgac
     7381 caaagagacc ggaaccggct tgggcttggc gattgtcaaa agagtcgtcg acgagcatcg
     7441 cggcgtcatc ggcgtcgaat cgagattaca cgaaggcact acttttacgg ttcgcctgcc
     7501 gaaagcgtaa aacaattacg aattacgaat gaagaaaatt cttcggcaaa tggctcgaat
     7561 ctttcttcca attcgtaatt cgtaatttgt aattcgtaat tctcgattac ccgttcacgg
     7621 tcgcctgcgc gacatcgctc caatcgcctt tgtcgccgtt tttcgactgc cagcgcagca
     7681 gataataaac ggtttttccg gcgtccgcgc cgtcaaactg gatgacataa ggcgtcgccg
     7741 tatcgagcgc gagaaactgg cattccttat tatcgacggg cgctgtgccg ccgattttat
     7801 accagatttc cgcgcctaag attccgccgg gctttttgtc cgtatcgggc gtcgccgcgt
     7861 cgcgaaaatt taaggtgtgc cgcagcttgc ccaaatcaat cagcgcaaac ggcacgctga
     7921 ccggcgcgcc cgcctccgtc cgcgtctcgt ccaaagggtc taaattgacc ttgcccagct
     7981 tgtccgcgtc gttgccgacc gtgctgcgaa tcaatcccat ctgctttcgc gtcagctcga
     8041 taattttcgc ccgccgatta tttttcgcgc cccgcgctgc attttccgac gcttccaaac
     8101 cttccaacgt cgccaaatcg tcgtcgaatc cgtcaagctc agcctttaaa gccgccgtca
     8161 gcgacgccgt cagcccgtaa ctcgcctcgt cgccggtcgc gttatcattc atcaaacgaa
     8221 cctgcgccga cagctcgtgg tcggaaagcc tgtttaagtc tctcgctttc atacaatttt
     8281 ttctccatta aatttgattt ggcggtaaat cgagaatgga taaaaggttg ccggttttct
     8341 ttgagctgcg cggttagtta aaaaattcgc cgtgaaagtt ttttttgatt caatctgcat
     8401 tcacgtgggc gaaaggtttg cgcagaagaa caaagaaaca tttatttatc ttgaaccgca
     8461 ataaatatat aaaccgaatt ttaatttttg gcaagatttt ttttcaaacg gactcggaca
     8521 aaccattttc ggtgcaagac aaacgccgtt tgaaaaaaga cagacgccgc ttgtccgaag
     8581 acaaaccgca tttg
//
LOCUS       DASWNE010000511         1577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_613554, whole genome shotgun sequence.
ACCESSION   DASWNE010000511 DASWNE010000000
VERSION     DASWNE010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613554"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="VGB68_10140"
     CDS             <1..1161
                     /locus_tag="VGB68_10140"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEY0049635.1"
                     /translation="FILYTSGTTGKPKGILHTTGGYLTQTFITSKWVFDLKDEDTYWC
                     TADIGWVTGHSYVVYGPLANGATVFMYEGAPNHPEPDRFWRIIERHKINIFYTAPTAI
                     RAFIKWGEQYPLKHDLSSLRLLGTVGEPINPEAWMWYHTIIGKKKCPIVDTWWQTETG
                     AIMISPFPGATPTVPGTATRPFPGILIDIQTRDGKSVGTNEGGYLVVTYPFPSMLRTI
                     WGDDERFKKQYWSEIPGVYFAGDGARRDQRGYYWIMGRVDDVINVSGHRLGTAEIESA
                     LVSHPAVAEAAVVGRPDEIKGQAIAAFVTLEGGRQGNEALKQELRAHVAKEIGSLAKP
                     DDLRFTDTLPKTRSGKIMRRLLRELASGGTVAGDVTTLEDFSVLEKLRRDEE"
     gene            1314..>1577
                     /locus_tag="VGB68_10145"
     CDS             1314..>1577
                     /locus_tag="VGB68_10145"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0049636.1"
                     /translation="MMQSSVDSSATTVFDAPALLNFTQKTTAKPLSLKYHIVFHLDKD
                     CVIGEEWRKMFYKYIAGCLRALRCDLEAIGGADDHVHLLVNLNS"
BASE COUNT          386 a          402 c          427 g          362 t
ORIGIN      
        1 tttattcttt acacatcggg cacgaccggc aaaccgaaag gaattctgca cacgaccggc
       61 ggttatttaa cgcaaacctt tatcacttca aaatgggttt tcgatttaaa ggacgaagac
      121 acatattggt gcaccgctga cattggctgg gtcaccggac acagttacgt cgtttacgga
      181 ccgctggcga acggtgcgac cgtttttatg tatgaaggcg cgccgaatca ccctgagccg
      241 gacagattct ggcgtattat cgagcgccac aaaatcaata ttttttacac tgcgccgacg
      301 gctatccgcg cgtttatcaa gtggggcgag caatatccgc taaaacacga tttatcgagt
      361 ctgcgccttt taggcacagt cggagagccg atcaacccgg aagcgtggat gtggtatcac
      421 acgattatcg gcaagaaaaa atgcccgatc gtcgatacat ggtggcagac cgaaaccggc
      481 gcgattatga tttcgccgtt tccgggcgca actccgaccg ttcccggtac ggcgacgcga
      541 ccgtttcccg gcattttgat tgatattcaa accagagacg gaaaatccgt cggcacgaac
      601 gaaggcggtt atctggtcgt cacctacccg tttccctcga tgctgcgcac gatttggggc
      661 gacgacgaac gcttcaaaaa acaatactgg tcggagattc cgggcgttta tttcgcgggc
      721 gacggcgcgc gacgcgacca gcgcggctat tactggatta tgggacgcgt ggacgacgtg
      781 ataaacgtct ccggtcatcg tctgggaacg gcggaaatcg agagcgcgct ggtttcgcat
      841 ccggcagtgg cggaagcggc ggtcgtcggc agacccgacg aaatcaaagg tcaggctatc
      901 gccgcctttg ttacgctcga aggcggtcgg caaggcaacg aagcgttgaa gcaggagctt
      961 cgcgctcacg tcgccaaaga aatcggctcg ctggcaaagc ctgacgattt gcgtttcacc
     1021 gacactctgc cgaaaacgcg cagcggaaaa attatgcgcc gcctgctgcg cgaactggcg
     1081 tcgggcggaa cggtcgccgg cgacgtcacg acgctcgaag atttttccgt tctggaaaaa
     1141 ctgcgccggg atgaagaata gtcagaaatg caaagtgcaa aatgaaatca cttcttctat
     1201 tttgcatttt gcattaactt tactacccaa acagggggtg aaattgacgc cgataatcaa
     1261 ttatcttaaa tgcggctcgg gaatattttc ccgcaaaagg agattttgat tatatgatgc
     1321 aatcatctgt tgactcttcg gcgaccaccg ttttcgacgc tccggcgttg ttgaatttca
     1381 cccaaaagac gacggcaaaa cctttgtcgc tgaaatatca tatcgttttt catctcgata
     1441 aagactgcgt cattggcgaa gaatggcgaa agatgtttta taaatatatc gccggctgcc
     1501 tgcgcgctct gcgctgcgat ttggaggcga taggcggggc tgacgaccac gttcatctgc
     1561 tggtcaatct gaattcg
//
LOCUS       DASWNE010000512         2188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_653127, whole genome shotgun sequence.
ACCESSION   DASWNE010000512 DASWNE010000000
VERSION     DASWNE010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653127"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..1106)
                     /locus_tag="VGB68_10150"
     CDS             complement(204..1106)
                     /locus_tag="VGB68_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049637.1"
                     /translation="MSNDKLQKKAPDSLKEKSQYALAFGVIAMMLFAVGFPMFVIAFF
                     GIFAFFLWKTFATSSPNGVREVFEFYLSANEILRDDERRWFGFEVQEVIVRGERILQT
                     MNGAPPLVYFTLGALYNKLGDHKSAVNHLAYVVENENSDESTYNYPSPELRNYTKILR
                     KIEREPADAPQTSAAVRALERARRNRGKALLEQSRDAVKEFNKKKRESAAEQRRLKAE
                     TAAQNGKDAFLSTVSATGIESFAENTEKPEPALPRQPVQSVTSAEEPAKENGNGSKEQ
                     NQNDLYNQRKPISEVLNDLYGENK"
     gene            complement(1106..1786)
                     /locus_tag="VGB68_10155"
     CDS             complement(1106..1786)
                     /locus_tag="VGB68_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY0049638.1"
                     /translation="MEAEIKFEPEGRSGVVATGSYLFDAAKRLGVDIPECERRGESDL
                     CAVQILSGKNLLSEPTKAELETLGSDRFIKGERLACQAKVVAAGEIVVMAEKKKKTTE
                     EEKKSEARKKVEDFRKEFDEMPLEKKIAALLELEAVALGETVSFVLNSPYHIFGKIMD
                     VMAEFGLKIDKEVKDAKTPAEHKAQEEKAETNGDGSTAETDSEDTNKDKKSSRGSKKA
                     SEENSQAE"
     gene            complement(1862..>2188)
                     /locus_tag="VGB68_10160"
     CDS             complement(1862..>2188)
                     /locus_tag="VGB68_10160"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEY0049639.1"
                     /translation="YRTLKLLTEAGLAREVRFGDGKTYYEHHYNHEHHDHMICTECGK
                     VIEFYSPEIEALQDAMADKFNFKPTHHSLRILGVCAECQAKESDIHMTASGAQPRVRV
                     MASKNK"
BASE COUNT          429 a          587 c          540 g          632 t
ORIGIN      
        1 ccgatttcat ttagtttttc ggtcagccag aggctgttgg tatcggtttt gtcgggagtc
       61 agaagctcgg agccgatggc gataatttcc gctgtaagca taagaaaaaa tattaccaca
      121 gagataagat atttgaaggg caaaaggcga aaccgaaaag ccggcttttc ggtttcgcct
      181 tttgcccttt aacttttacc ttcttatttg ttctcgccgt aaaggtcgtt taaaacttcc
      241 gagatcggct tgcgttgatt gtagaggtcg ttctggtttt gttccttcga cccgttgccg
      301 ttttccttcg ccggttcttc ggcgctagtc acggattgaa cgggctggcg cggtaaagcc
      361 ggctcgggct tttccgtatt ttccgcgaaa gattcgatgc ccgtggcgga aacggtcgac
      421 agaaacgcgt cttttccgtt ttgcgcggcg gtttcggctt tgagacgacg ctgctcggca
      481 gccgattcgc gtttcttttt gttgaattcc ttgaccgcat cgcggctttg ttccagaagc
      541 gctttgccgc gattgcggcg cgcgcgctcc agagcgcgaa cggcggcgga agtctgcggc
      601 gcgtccgccg gctcgcgctc gatttttcgc aaaattttcg tgtagtttct cagttccggc
      661 gagggataat tataggtcga ttcgtcggaa ttttcgtttt ccacgacata tgccaggtga
      721 ttgacggcgg atttgtggtc gcccagcttg ttgtaaagcg cgccgagcgt gaaataaacc
      781 agcggcggcg cgccgttcat cgtctgcaag atgcgctcgc cgcgcacgat gacttcctgc
      841 acctcaaacc cgaaccagcg ccgttcgtca tcgcgcagaa tctcgtttgc cgacaggtaa
      901 aactcaaaaa cctcgcgcac gccgttcggc gaagaagtgg cgaaggtttt ccatagaaaa
      961 aacgcgaaaa tgccgaaaaa agctatcacg aacatcggaa agccgacggc gaaaagcatc
     1021 atcgcaatca cgccgaaagc cagcgcatac tgcgattttt ccttcaaaga gtcgggagcc
     1081 tttttttgaa gtttatcgtt gctcactatt ccgcctgaga attttcctcc gaagcttttt
     1141 tactgccgcg gctggacttt ttgtctttgt tcgtgtcttc cgagtcggtt tcggcggtcg
     1201 agccgtcgcc gttggtttcg gctttttctt cttgcgcttt atgttcggcg ggcgttttgg
     1261 cgtcctttac ttctttatca attttcagac cgaattccgc catcacgtcc ataatcttcc
     1321 cgaaaatgtg atacggcgaa ttcagtacaa aagaaaccgt ttcgccgagc gcgacggctt
     1381 ctaactcgag cagcgccgcg attttcttct ccagcggcat ttcgtcaaac tccttgcgaa
     1441 aatcctcgac ctttttgcgc gcttccgact ttttctcttc ttcggttgtc ttttttttct
     1501 tttccgccat aactacgatt tctcccgcgg cgaccacttt tgcctggcag gcaagccgct
     1561 cgcccttgat aaatctgtcc gagccgagcg tttccagctc ggctttggtc ggctcggaaa
     1621 gcagattttt tccgcttaaa atttgcacgg cgcacaaatc cgattcgccg cgtcgctcac
     1681 attcgggaat atcgacgccc agacgctttg ccgcgtcgaa aagataactg cccgtcgcga
     1741 ccacgccgct ccgtccttca ggttcaaatt taatttctgc ttccattttt tctttcttag
     1801 ttgttagtta tcagttgtta gttgtcagtt ctgaatttaa ttcaaccggc aaccgtctaa
     1861 tttatttatt tttactcgcc atcacgcgca cgcgcggctg cgctcccgaa gccgtcatat
     1921 gaatatcgct ttcctttgcc tggcattcgg cgcaaactcc cagaatacgc aggctgtgat
     1981 gcgtcggctt aaagttgaat ttatccgcca tcgcgtcctg cagcgcttcg atttcgggcg
     2041 agtaaaactc gatgactttt ccgcattcgg tgcaaatcat atggtcgtga tgctcgtgat
     2101 tgtaatgatg ctcgtaatat gttttgccgt cgccgaagcg aacttcgcgc gccagaccgg
     2161 cttcggtcaa aagcttcagc gtgcgata
//
LOCUS       DASWNE010000513         2016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_265297, whole genome shotgun sequence.
ACCESSION   DASWNE010000513 DASWNE010000000
VERSION     DASWNE010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265297"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VGB68_10165"
     CDS             complement(<1..581)
                     /locus_tag="VGB68_10165"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEY0049640.1"
                     /translation="MEIFDINPDIRRAETLSSEFYTDERYFQESKEKIFARSWQLLGT
                     TSEINNLKPHTLLENFLDEPVLISRAAGGFHCLSNVCTHRGKILIEKDCRANLIRCGY
                     HGRRFSLDGKFLSMPEFERVENFPAERDNLPRIPFGVWGKFLFAAVNPIAPLEDFLAE
                     MQKKIRVSDFENLRFAGARDYEVKAHWSLYCEN"
     gene            complement(737..1492)
                     /locus_tag="VGB68_10170"
     CDS             complement(737..1492)
                     /locus_tag="VGB68_10170"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0049641.1"
                     /translation="MTKPEIEIREVTTVEELAACVQLQREVFASPDLEISPVRHLIVT
                     RQAGGWTLGAFAGGALVGFVLSVPMFRAGGERAFYSHMTAVREEFQNYGIGARLKWAQ
                     RERALLEGINFIKWTYQPVQARNAFFNLERLGVSVKTYMPNFYGTDYSTSAEQNEIIG
                     LDSDRLFAEWQLDSPKVLVLSKGEKFVETEKVALTIEIPNHWNNLVTANPEKAIAEQT
                     RIKQEFQKAFAGGLICKGFERNETSPRYLLFEE"
     gene            complement(1519..1977)
                     /locus_tag="VGB68_10175"
     CDS             complement(1519..1977)
                     /locus_tag="VGB68_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049642.1"
                     /translation="MLHDFTLTGKRVRLWQRPGESYGHVLMKALGWAMFAGKYPHLEI
                     EVKVGLRYKPDLIAARENGDFDFWGECGANSLRKTGWILKHTRTKKLVLFKIGVNAEP
                     IIKQLRAEIPAKYRAAGKLVLINFISDIVNLTASKQIEKVSADWFDKFEI"
BASE COUNT          476 a          523 c          485 g          532 t
ORIGIN      
        1 taattttcgc aatacaaact ccagtgagct ttgacctcat aatcccgcgc tccggcaaag
       61 cgcaggtttt caaaatctga gactcgaatt tttttctgca tttcggcgag aaaatcttcg
      121 agcggcgcga tcggattgac cgcggcaaac aaaaattttc cccagacgcc gaacggaatt
      181 cgcggcagat tgtctcgctc ggcgggaaaa ttttcgaccc gttcaaattc gggcatcgaa
      241 agaaattttc cgtcgagcga aaatcgcctg ccgtgatagc cgcagcgaat cagattggcg
      301 cggcaatctt tttcgattaa aatttttccg cgatgcgtgc agacgtttga aagacaatga
      361 aaaccgcccg cggcgcgcga aatcaaaacc ggctcgtcga gaaaattttc gagcagcgtg
      421 tgcggcttga gattgtttat ctcgcttgtc gttcccagaa gctgccagct gcgcgcaaag
      481 attttttcct tcgattcctg aaaataccgc tcgtccgtgt agaattcgga cgaaagcgtc
      541 tcggcgcggc gaatgtcggg attgatgtcg aaaatttcca taaattttgc cgccgcgcgc
      601 agacagagag aggaagagaa acgcagagaa aaatttaacc ggatgagcgg gatatttacg
      661 gataaatgaa ttttccgttc gtcatctttc gcgtttgcgg ctgatttttc tttctctgtg
      721 ctcgcgcggc ggttaattat tcttcaaaca gtaaatatct ggggctggtt tcgtttcttt
      781 cgaagccttt gcaaatcaag cctccggcaa aagccttttg aaattcctgt ttgattctcg
      841 tttgttcggc gatggctttt tcgggattcg ccgtgaccag attattccaa tgattcggga
      901 tttctatggt cagcgcgact ttttccgttt cgacaaactt ttcgcctttt gaaagcacca
      961 ggactttcgg cgaatccagc tgccattcgg caaagagccg gtcgctgtcg agcccgatga
     1021 tttcgttctg ctcggcggaa gtcgaataat ccgtgccgta aaaattcggc atataagttt
     1081 tgacgctgac gccgaggcgt tcgagattga aaaaagcgtt acgcgcctgc acgggctgat
     1141 acgtccattt aatgaaattt atgccttcga gcagagcgcg ctcgcgctgc gcccatttta
     1201 aacgcgcgcc gatcccgtaa ttttgaaatt cctcgcggac ggcggtcata tgcgaataaa
     1261 acgcgcgctc gccgccggcg cgaaacatcg gaacgctcag gacaaagccg accagcgcgc
     1321 cgccggcaaa tgcgccgagc gtccagccgc ccgcctgccg ggtcacaatc aaatgccgca
     1381 cgggcgaaat ctccaaatcc ggcgaagcga aaacttcacg ctgcagctgc acgcacgcgg
     1441 ctaattcttc gaccgtcgtc acctcgcgga tttctatctc gggttttgtc atctcgttta
     1501 aatttcagat ttcaaatctc aaatttcaaa tttatcgaac cagtctgctg aaactttctc
     1561 aatttgtttc gaggctgtca aattcacgat atcggaaata aaatttatca aaacgagttt
     1621 tccggcggcg cgatatttgg cgggaatttc ggcgcgcagc tgcttgataa tcggctcggc
     1681 gttaactcca atcttgaata aaaccagttt tttcgtgcgc gtgtgtttta aaatccagcc
     1741 ggttttgcgc aagctgttcg cgccgcattc gccccagaaa tcaaaatcgc cgttttcgcg
     1801 ggcggctatc aaatcgggtt tgtagcgaag cccgactttc acttcaattt ccagatgcgg
     1861 atatttcccg gcaaacatcg cccagccgag cgctttcatc aaaacgtgcc cgtaagattc
     1921 gcccggtctc tgccacaagc gaacgcgctt tccagtcaat gtaaaatcgt gaagcatccg
     1981 ttttgccttt tattttttct ctgcctgcgg ttaaac
//
LOCUS       DASWNE010000514         6308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_509860, whole genome shotgun sequence.
ACCESSION   DASWNE010000514 DASWNE010000000
VERSION     DASWNE010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6308
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509860"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..494)
                     /locus_tag="VGB68_10180"
     CDS             complement(57..494)
                     /locus_tag="VGB68_10180"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY0049643.1"
                     /translation="MDAIDWKILRELQRDARISYAELGRRVGLTTPAVIERVRKLEDA
                     GIITGYRAEIDAAKVGLPITAFIRMSITGVDYSRIIEVAENAPEVLECHRGTGGDSFI
                     MKVAVSSVEHLQNLIDRLTPYGITTTSIVLSSPVKSRVIEKKD"
     gene            complement(848..1813)
                     /locus_tag="VGB68_10185"
     CDS             complement(848..1813)
                     /locus_tag="VGB68_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049644.1"
                     /translation="MKLRILYHGNCFDGVSSAAFFTKFYRSKFNANTEIEYQPMAHRA
                     GNLFDRESLAGDGENAIVDFKYSPDERVTWWFDHHQSAFLTEADERHFQADTSGKKFL
                     DTQSKSCCEFIARIAKEKFAFEDESLDEMVEWAHIIDGALYESPAQCVELRSSALKLM
                     QVIEGEKDARFVEKIIRDLTEKSLDEVVESEDVQAKLKPILEQHWNTAEIIKQKSKYS
                     RGVVSFDLVEDGIEGYNKFIPYYFYPQTTYNVAILRSSFRTKISVGSNPWSPRPRLHN
                     IAKICERYGGGGHAVVGAVSLPPEDVELGRKYMREIIEELQFETD"
     gene            1897..2355
                     /locus_tag="VGB68_10190"
     CDS             1897..2355
                     /locus_tag="VGB68_10190"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0049645.1"
                     /translation="MNNKILIRLANKRDAAAMVEFNQAMAQETEGKFLDKAILKGGVE
                     AVFDGAGKGFYVVAESENKIVGGLMVTFEWSDWRNSWFWWIQSVYILPDFRGRRIYRR
                     LYEFVKQKAAEAKNVCGFRLYVEKDNARAQQVYEKSGMEQSHYLMYEEMI"
     gene            3113..3397
                     /locus_tag="VGB68_10195"
     CDS             3113..3397
                     /locus_tag="VGB68_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049646.1"
                     /translation="MTKISEKEFARICAGIYEDGESIYRHNPLGTREETLLWMLLSVL
                     LSYLSLSEIETPCFNGKPTAETYRQAILFVLRDRKTDDFEAEKYLVKLSE"
     gene            3418..4110
                     /locus_tag="VGB68_10200"
     CDS             3418..4110
                     /locus_tag="VGB68_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0049647.1"
                     /translation="MIIETFVTTAFQQNTRLLACAETRQAICIDPGGDADIIAGYLNK
                     NDLQLQAVTLTHAHLDHIGGTSDLHKSFPHVEILLHRDDEDLYYLLPAQPQALGIPRS
                     AWANLGFEYENPPKITRNWQDGEIYEVGRLRFKVLHCPGHTRGHVVLFEERERKVFVG
                     DCLFAGSIGRTDLPGGSYEQLMDSITNKILTLGDDVTVYSGHGAETTIGHEKQTNPFL
                     TGHYGLGRGRFI"
     gene            complement(4178..4900)
                     /locus_tag="VGB68_10205"
     CDS             complement(4178..4900)
                     /locus_tag="VGB68_10205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEY0049648.1"
                     /translation="MIIRCDNCSVSLQLDESKTPNGNFTVRCPRCQNLIRVGGGKGAS
                     TINQLKENAPAPAMPDGAQGFKTKESGFEINHALRSLLSALQMENKILDSDDTSDEKP
                     RRVLLCLGQHRDRVAKMMAESGYKVYLAQTPAQANERMREGKTEILIFSPDFASEFGG
                     ATILQQKMNSMYSSERRRLYLVSLEESGTTLNAHDAFLRNLNLIVNTSDIDQLPLILN
                     RALRDFNDLYFYLNKAVGTAAI"
     gene            complement(5027..6226)
                     /locus_tag="VGB68_10210"
     CDS             complement(5027..6226)
                     /locus_tag="VGB68_10210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEY0049649.1"
                     /translation="MSLLDIAPIIEQMLLISDNVSDLNFSVGQKPQVEISGALYSASP
                     LGLSKLSPFQTEMIAMAILRDSPEAAAQLAHSGTADLSWSLPSRCRFRVNIFQQRNSY
                     SIVMRVIPHDIPTFQSLNVPPQLEDICGIRNGLVLVTGPTGSGKSSTLAAVIDRINET
                     KSYHIVTIEDPIEFLHSHKKSTINQREVGADTKDFASALRAALRQAPKVILVGEMRDL
                     ETAEIALEAAETGHLVLSTLHTIDAAKTVDRIIGLYPKNEEKIIRMRLAQTFRYILSQ
                     RLIPKASGKGRVAAVEILKSNPRTREYIERGESDGKTLLDAMRDGEIDGMQDFDTVIR
                     RMIEQEMITLEDGLSFATNQNNLLLQLKGLSSTEDYANAPKPKTNGVALPIPPPVPKN
                     SVLDMIE"
BASE COUNT         1609 a         1519 c         1518 g         1662 t
ORIGIN      
        1 ccggcgtcgg cggatttttt aattctttgt tcgcgccttt tcgtgctctt tcgcggttaa
       61 tctttcttct caatcactcg gcttttcacc ggcgaggaaa gcacgatcga agtcgtggtg
      121 atgccgtagg gcgtcagccg gtcaatcaga ttttgcaggt gttcgacgct ggaaacggcg
      181 actttcataa taaacgaatc gccgcccgtc ccgcgatggc attccagaac ttccggcgca
      241 ttttcggcga cttcgataat acggctgtaa tcgacgcccg taatgctcat ccggataaaa
      301 gccgttatcg gcaagccgac tttcgccgcg tcgatttcgg cgcgataacc cgtaatgatt
      361 ccggcgtctt ccagttttct gactctttcg ataacggcgg gcgtcgtcaa accgacgcgg
      421 cgaccgagct cggcataact gatgcgcgcg tcccgttgca attcccttaa aattttccaa
      481 tcgattgcgt ccatttattc aaaattgaac attaaactaa gtttttacct gacgaaaata
      541 aacttttact tagggaaaaa cttttgaagc ttagggaaaa aaagtttttc cttaggtttt
      601 agaaactttt cgctaggttt taagtttctt tccctaagtt tcagaacctt ttcgccagat
      661 tttaaaagtt ttcccctaag ctggaaaaca atttccctgt taaaaaacta tttttcccta
      721 tcgaaaaact ttatttcccc gattccgaaa agtttttaat cagcaaaaaa ctattattaa
      781 ccgtcaaaat cggcgaaatg tcgatttaaa agaattcatc gttcatcgtt ccgcgctcat
      841 cgtttcgcta atcggtttca aactgtaatt cttcgataat ctcgcgcata tattttctgc
      901 ccagttcgac gtcttccggc ggaagcgaaa ccgcgccgac caccgcgtga ccgccgccgc
      961 cgtagcgttc gcagattttg gcgatattgt gcaggcgcgg gcgcggcgac cacggattcg
     1021 agccgaccga gattttcgtg cggaagctgc tgcgcagtat cgcgacgtta taagtcgtct
     1081 gcggataaaa ataatacggg ataaatttgt tgtagccttc gattccgtcc tcgacaagat
     1141 cgaagctgac gacgccgcgc gaatatttcg atttttgttt aataatttcg gcggtattcc
     1201 aatgctgttc gagaatcggt ttcagcttcg cctgaacgtc ttcgctttcg accacttcgt
     1261 cgagcgattt ttcggtcaaa tcgcgaatga ttttttcgac aaaccgcgcg tctttttcgc
     1321 cttcgataac ctgcatcaat tttaaagccg acgagcggag ctcgacgcat tgcgcgggcg
     1381 attcatacag cgcgccgtcg ataatgtgcg cccattcgac catttcatcc agcgattcgt
     1441 cttcgaaagc gaatttttcc ttcgcaatcc gcgcgataaa ttcgcagcag gatttgctct
     1501 gggtatcgag aaattttttt ccgctcgtgt cggcttgaaa atgccgctcg tccgcttcgg
     1561 tcagaaaagc cgactggtga tggtcaaacc accacgtcac gcgctcgtcg ggcgaatatt
     1621 tgaaatcgac aatcgcgttt tcgccgtcgc cggcgagcga ttcgcggtcg aaaagatttc
     1681 cggcgcggtg cgccatcggc tggtattcga tttccgtatt tgcgttaaat ttcgaacgat
     1741 agaatttggt aaaaaaagcg gcggaagaaa cgccgtcaaa gcagtttccg tgataaagaa
     1801 tgcgaagttt catcgattat tggtatgatt ccttttagta aaaatcacaa acatttcaga
     1861 gtagttggct caaatggaaa cgtcaaggca ggatttatga ataataaaat tttaattcgt
     1921 ctggcaaata aacgggatgc ggcagcgatg gtcgaattta accaggcgat ggcgcaggaa
     1981 acggaaggaa aatttttaga taaagcgatt ttaaagggcg gcgtcgaagc ggttttcgac
     2041 ggcgcgggca aaggtttcta cgttgtcgcc gaaagcgaaa acaaaatcgt cggcggcttg
     2101 atggtcacgt ttgaatggag cgattggcgc aattcctggt tctggtggat tcagagcgtt
     2161 tatattctgc cggattttcg cgggcgcagg atttaccggc ggctctacga attcgtcaag
     2221 caaaaagccg ccgaagcgaa aaacgtctgc ggctttcgcc tctatgtcga aaaggataat
     2281 gcgagagcgc agcaggttta tgaaaaatca ggaatggagc aatctcacta tttgatgtat
     2341 gaagaaatga tttaaataaa aaaccgaagc ccttttcagg acttcggttt cggctacaat
     2401 gaaaggaaat ttgagaaaca caggctcggc atataattat aaggagcaaa ataatcaaaa
     2461 acctgagcct gtgttataaa acgaaaaaaa acagcgtttc tcaaactctt tcaaaaaatc
     2521 caaaaaatcg ccttttgtgc tctcagcaca tttttttgac aatcggcgga taataaaacg
     2581 aaccgtgtgt ctgtaacgtt tccgtattga actgttttct cgcgccgcaa tgaagacaag
     2641 ttctataagc cgtattgttc tgtacgaaag gacgacttaa atcttcgtgc caacatccaa
     2701 ataatttacc gagaactccg actttgtcac caaataccgt ttggtcaacc ctgtttttta
     2761 catcctgaag tacattttct aatgaaatca tctgcattcg cgtaactccc cctatcagct
     2821 gcggcaaaaa gccctgccta agccttttgc cccgccgcca tactcatccg agcgttcgtt
     2881 taatgccttc cgtgacgttt actttagcaa gaaatgtgcc aaaactcggt tgaaagtaaa
     2941 aaaatcgata agtccaaata tttcagaaat aaagtagttt taggtgagat tttagtataa
     3001 atatcgtgtc cgctttacat acatcgcatc gtcattttaa atcttgtgtt tgaaaacgat
     3061 tcagtcagtt tgaatgaatt gcgttccggt cattaaactt ttactttgag atatgacaaa
     3121 aatcagcgaa aaggaatttg cgcgaatctg cgcgggaatt tatgaagacg gcgaaagcat
     3181 ctaccggcac aatccgctcg gaacgcgcga agagacgctt ctgtggatgc ttctgagcgt
     3241 tttgctcagt tatctgagtc tttccgaaat cgaaacgccg tgtttcaacg gcaaaccgac
     3301 ggcggaaact taccgacagg cgattttatt tgttttacgc gatagaaaaa ccgacgattt
     3361 tgaagcggaa aaatatttag taaaattatc cgaataaatt tacatcatca aaataaaatg
     3421 attatcgaaa cctttgtcac caccgcattt caacaaaaca cgcgccttct ggcgtgcgcg
     3481 gaaacccgtc aggcgatttg catcgacccg ggcggcgatg ccgacataat cgccggatat
     3541 ttaaataaaa acgatttgca gctgcaagcc gtcacgctga cgcacgcgca tctcgaccac
     3601 atcggcggca cgagcgattt acacaaaagt tttccacatg tggaaatcct tttgcacagg
     3661 gacgacgagg atttatatta ccttctgccc gcgcagccgc aggcgctggg aattccgcgc
     3721 tcggcgtggg caaatctcgg tttcgaatac gaaaatccgc cgaaaattac acgcaactgg
     3781 caggatggcg agatttacga ggtcggcagg cttcgtttta aagttttgca ttgccccggt
     3841 cacacgcgcg gacacgtcgt tttgtttgaa gaacgcgaaa gaaaagtttt tgtcggcgat
     3901 tgtctgtttg ccggctcaat cgggcggacg gatttgcccg gcggaagcta cgagcaatta
     3961 atggattcga ttacgaacaa aattctcacg ctcggcgacg acgtgacggt ttattccggt
     4021 cacggagcag aaactacaat tggacacgaa aaacagacga acccgttttt gacgggacat
     4081 tacgggctcg gacgcggaag atttatttaa aaaaaagtgc tgagttccga ggaaaagcca
     4141 actcagaact cagcacttta cactcagcac ttttttctca gattgccgcc gtgccgacag
     4201 ctttattcag ataaaaatac aaatcgttaa aatcgcgcag ggcgcggttt agaatcaaag
     4261 gcagctggtc gatgtcgctc gtgttgacga ttaaattcaa atttcgcaga aaggcgtcgt
     4321 gggcgttcag cgtggtgccg ctttcttcga gcgagaccag atacaggcgg cggcgttccg
     4381 acgaatacat cgagttcatt ttctgctgca aaatcgtcgc tccgccgaac tccgaagcaa
     4441 aatcgggcga aaatatcagg atttccgttt tgccttcgcg cattcgctcg ttggcttgcg
     4501 cgggcgtctg cgccaggtaa accttgtagc cggattcagc catcattttg gcgacgcggt
     4561 cgcggtgctg tccgagacaa agcagaacgc gacgcggttt ttcgtccgaa gtatcgtcgg
     4621 aatccagaat tttgttttcc atctgcaagg cggaaagcag cgagcgcaaa gcgtgattta
     4681 tctcaaatcc ggattctttt gtcttaaacc cctgcgcgcc gtcgggcatc gccggagcgg
     4741 gcgcgttttc cttcaactgg ttaatggtcg aagcgccttt tccgccgccg acgcgaatca
     4801 gattttgaca gcgcggacag cgaaccgtaa aattcccgtt cggcgttttc gattcgtcga
     4861 gttgcagaga aaccgaacaa ttgtcgcagc gtattatcat aaatttttat cggtctttgg
     4921 tctttggtct tcggtcttcg ttaaggaatt tgctcaaggt tgagcagtaa aaaaagtcga
     4981 agaccaaaga ccgaagacca aagaccattt tcaaaattga aaatatttat tcaatcatat
     5041 cgagcaccga gtttttcggc accggcggcg gaatcggcag agcaactcca ttggttttcg
     5101 gcttcggcgc gttggcgtaa tcttcagtcg aagaaagccc tttcaactgc agcaaaagat
     5161 tgttttggtt ggtcgcaaac gaaagcccgt cttcgagcgt aatcatttcc tgctcaatca
     5221 tccgccgaat caccgtatcg aaatcctgca ttccgtcgat ttcgccgtcg cgcatcgcgt
     5281 cgagcagcgt ctttccgtcg ctttcgcctc tttcgatata ttcgcgcgtg cgcggattcg
     5341 attttaaaat ctcgaccgcc gccacgcgac ctttgccgct cgccttcgga atcagacgct
     5401 gcgagagaat gtatcgaaac gtttgcgcca gccgcatacg aatgattttt tcttcgtttt
     5461 tcggatacaa accgatgatg cggtcgaccg ttttcgccgc gtcaatcgtg tggagcgtgg
     5521 ataaaacgag gtgtccggtc tcggcggctt cgagcgcgat ttccgccgtt tcgaggtcgc
     5581 gcatctcgcc gaccagaatg actttcggcg cctggcgcaa agcggcgcgc agggcggagg
     5641 cgaaatcttt cgtatccgca ccgacttcgc gctggtttat cgtcgatttt ttgtgcgagt
     5701 gcagaaattc gatcgggtct tcgatcgtca cgatatggta gcttttcgtc tcgttgatgc
     5761 ggtcgatgac cgccgcgagc gtcgagcttt ttccggagcc ggtcggaccc gtaactaaaa
     5821 ccagaccgtt gcgaatgccg caaatgtctt cgagctgcgg cggcacgttc agcgattgaa
     5881 aagtcgggat gtcgtgcgga atcacgcgca ttacaatcga atacgagttt cgctgctgaa
     5941 aaatattgac gcgaaagcga cagcgcgacg gcaggctcca gcttaaatcc gcggttccgc
     6001 tgtgcgccag ctgcgcggcg gcttccgggc tatcgcgcag gatagccatc gcaatcattt
     6061 cggtttgaaa aggcgacagt tttgacagac cgagcggcga agccgaataa agtgctccgc
     6121 tgatttccac ctgcggcttt tgtccgacac tgaaattcaa atcgctgaca ttatcggaaa
     6181 tgagcagcat ctgctcaata atcggtgcta tatccagcaa actcattttt ggaaattact
     6241 ccttggacaa atcgtgacag aggattcagg caagctatga agtttttaaa tcacggttaa
     6301 attaaact
//
LOCUS       DASWNE010000515         2284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_653250, whole genome shotgun sequence.
ACCESSION   DASWNE010000515 DASWNE010000000
VERSION     DASWNE010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653250"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..1501)
                     /locus_tag="VGB68_10215"
     CDS             complement(149..1501)
                     /locus_tag="VGB68_10215"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0049650.1"
                     /translation="MSNSILIVDDERGIRETLRGVLEDEGFEVEAVEAGEDCLKVAAT
                     KNFGCILLDVWLPNIDGLETLKQLRENGSDSAVVMISGHGNIETAVNATKLGAFDFIE
                     KPLSIEKTILTIRNAIRTKELERLNAQLAETLSQEYVMIGDSIVMRALRKQIAIVAPT
                     DGRVLISGESGTGKELVARAIHAQSKRQNAPFVEINSAAIPEELVESELFGHAKGAFS
                     GALQAKKGKFEFADGGTLFLDEIGDMSPRVQAKMLRVLEEQRFEPVGSNTPLRVDVRV
                     ISATNKNLDELIESGGFRADLFFRLNVIPFQIPPLRERLEDIPALVEHFNRKFSDAYG
                     KRPKIFSESAIEALQNYAWLGNVRELKNTIERIVIMNSKRQISVGDLPMMSDEKPPAS
                     SYRFPSFRAATDAYQREFIQRKLAEADGNISKAAELMGVDRSHLHRRIKTLGIVTKGN
                     "
     gene            complement(1528..>2284)
                     /locus_tag="VGB68_10220"
     CDS             complement(1528..>2284)
                     /locus_tag="VGB68_10220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0049651.1"
                     /translation="AWAEVARRMAHEIKNPLTPIQLSAERIIKNFYRNSSFAARSSSA
                     DNEAKDLLAGGEGQRTKDKGQFEKVVEESTSTILREVNSLKSMVDEFSRFARLPNVIL
                     EDGSVNEIVRQSVALYEDRFSDVRIETLLARNLPSAMLDEEHLKRVFVNLIDNAIEAF
                     DKSQPDKRISVKSFHDKARGLIVAEVSDNGSGIAPSDFQKLFQPYFSTKGRGTGLGLA
                     IVQRIVIEHGGKIKAVNNSPRGAKFIIELPVLA"
BASE COUNT          489 a          636 c          576 g          583 t
ORIGIN      
        1 ataccgttcg gcaattgccg gaagccgttt tgaagctgcc aaatgctctg tggaagttgc
       61 cggaaggctt ccggcagctt caaaacggca gaatttgcat taaattaata caaaacagaa
      121 taaaccttct ttcattttcc attttccgtt aattaccttt tgtcacgatt cccaaagttt
      181 taatccgccg gtgcaggtgg ctgcggtcga cgcccatcag ctcggcggct ttcgagatat
      241 tgccgtcggc ttcggctagc ttgcgctgga tgaattcgcg ctggtaagcg tcggtcgcgg
      301 ctctgaaaga cgggaaacga tagctggacg cgggcggttt ttcgtctgac atcatcggca
      361 aatcgccgac ggaaatctgc cttttcgagt tcataatcac gatgcgctcg attgtgtttt
      421 tcagctcgcg aacgttgccg agccaggcgt aattctgcaa agcctcaatc gcgctctcgc
      481 taaagatttt cggtcgtttg ccgtaggcgt cggaaaattt acgattgaaa tgctcgacca
      541 gcgccgggat gtcttcgagc cgttcgcgca gcggcggaat ctgaaacgga atcacgttca
      601 ggcgaaaaaa caaatcggct cgaaaaccgc cgctttcgat gagctcgtcc agatttttat
      661 tggtcgcgct gatgacccgg acgtcgacgc gcagcggcgt gttgctgccg accggctcaa
      721 agcgctgttc ttcgagcacg cgcagcattt tcgcctgcac gcgcggcgac atatcgccga
      781 tttcgtcgag aaaaagcgtc ccgccgtcgg caaattcaaa cttccctttc ttggcttgaa
      841 gcgcgccgga aaacgcgcct ttcgcgtgtc cgaaaagctc gctttccacc agttcttcgg
      901 gaatcgcggc ggaatttatc tcgacaaacg gcgcgttttg ccgcttcgac tgcgcgtgta
      961 tcgcccgcgc gaccagttct tttcccgttc ccgattcgcc ggaaatcaaa accctgccgt
     1021 cggtcggcgc gacaatcgca atctgcttgc gcagggcgcg catcacgatt gaatcgccga
     1081 tcatcacata ttcctgactt aaagtttcgg caagctgcgc gtttaatctt tccagctctt
     1141 tcgtgcgaat cgcattgcga atggtgagaa tcgttttttc gatggacaga ggtttttcga
     1201 taaaatcgaa cgcgccgagc ttggtcgcgt tgacggcggt ctcgatgttg ccgtgaccgg
     1261 aaatcatcac gaccgccgaa tcgctgccgt tttcgcgcaa ttgtttcaaa gtttccaagc
     1321 cgtcgatatt cggcagccag acgtcgagca gaatgcagcc gaaatttttc gtggcggcga
     1381 ctttcagaca gtcttcgccc gcttcgaccg cttcgacctc aaagccttcg tcttccaaaa
     1441 ccccgcgcag cgtctcgcgg atgccgcgtt cgtcgtcaac gattaaaatt gaattagaca
     1501 ttatgaaaat ttgactgcta aattatttca ggctaaaacc ggcaactcga taataaactt
     1561 cgcgcctcgc ggagaattgt tgaccgcttt aatctttccg ccgtgttcga tgacgattct
     1621 ttggacgatt gccaagccca aaccggttcc gcgtcctttg gtcgaaaaat acggctggaa
     1681 aagcttctga aaatccgaag gcgcgatgcc gctgccgtta tcggagactt cggcgacgat
     1741 taaaccgcgc gccttgtcgt ggaaactctt gacgctaatg cgtttatccg gctgcgattt
     1801 gtcgaatgcc tcgattgcgt tgtcaatcag attgacaaaa actcttttca gatgttcttc
     1861 gtcgagcatc gccgacggca gattccgcgc cagaagcgtc tctatccgaa cgtcgctgaa
     1921 acggtcttcg taaagcgcga ccgactggcg cacgatttca ttgacgctgc cgtcttccag
     1981 tatgacgttc ggcaggcgcg caaaacgcga aaactcgtca accatcgact tcaggctgtt
     2041 gacctcgcgc aaaatcgtgg acgtgctttc ttcgacgact ttttcaaatt gccccttgtc
     2101 ctttgtcctt tgtccttcgc cgccggcgag caaatctttc gcttcattat ccgcacttga
     2161 agaacgcgcc gcgaaagacg aatttcgata gaaattcttg atgattctct ccgccgaaag
     2221 ctgaatcggc gtcagcggat ttttgatttc gtgcgccatc cggcgggcta cttccgccca
     2281 ggct
//
LOCUS       DASWNE010000516         2088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_300602, whole genome shotgun sequence.
ACCESSION   DASWNE010000516 DASWNE010000000
VERSION     DASWNE010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300602"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2068
                     /gene="uvrA"
                     /locus_tag="VGB68_10225"
     CDS             <1..2068
                     /gene="uvrA"
                     /locus_tag="VGB68_10225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY0049652.1"
                     /translation="AERGAAGSTKLKFSDRFLCKYDNTLYEEPEPRLFSFNSPFGACP
                     TCQGFGNSIGVDFDLVIPNPLLSLKEGAVDPFTKPQHDWAQKELLRFARNSDIPTDVP
                     FADLNEFQQKAIKDGAPGWRGIRGFFSWLETKKYKLHVRVFLAKYRGYTPCPECRGGR
                     LRQAARDVKIAEKNLPEIVNLSISDAGKFFDELALSEEREKIAEKLLLEIRRRLKFLV
                     DVGLDYLTLDRLASTLSGGEAQRIQLATNLGSSLVGALYVLDEPSIGLHPRDNTRLVN
                     LLKNLRDIGNTVLVVEHDEEMMRAADRILDIGLYAGELGGNVIFAGNFDDLLEDEESL
                     TAKYLRGEAEIKIPSQRRKPSKRKLEIVGAREHNLKAVDAEIPLDMLVCVTGVSGSGK
                     STLIHDIIYAGLKKQRGEWQGHVGLFKEIKGGDFVDDIILVDQSPIGRTPRSNPVTYI
                     KAYDAIREVFSSTNTAKTHGYDASHFSFNVPGGRCEVCQGSGTVTVEMQFLADVELTC
                     EECYGTRFKAEILDVRYKGKNIHEVLQMTIREALFFFKEVNKLVNKLKVLEAVGLGYL
                     RLGQSATTLSGGEAQRVKLAAHLAQKTKTKTLYIFDEPTTGLHFDDINKLLAAFRALL
                     DNGASLLVIEHNLDVIKTADYVIDLGPEGGIGGGCIVTTGTPEEIVKSENSHTGRYLK
                     DYLPQN"
BASE COUNT          513 a          570 c          566 g          439 t
ORIGIN      
        1 ggcggaacgc ggcgcagccg gttcgacgaa gctgaaattc tccgaccgct ttctctgcaa
       61 atacgacaat acgctctacg aagaacccga accgcgcctg ttcagcttca actcgccgtt
      121 cggcgcgtgc ccgacctgtc agggcttcgg caattccatc ggcgtggatt tcgatttggt
      181 gattccgaat ccgcttttgt ctctgaaaga aggcgcggtt gacccgttta ccaaaccgca
      241 gcacgactgg gcgcaaaagg aattgctgcg tttcgcccgc aacagcgata ttccgactga
      301 cgttccattt gccgatttaa acgagtttca gcaaaaagcg attaaggacg gcgcgccggg
      361 ctggcgcggc attcgcggat ttttctcgtg gctcgaaacg aaaaaataca agcttcacgt
      421 ccgcgttttt ctggcgaaat atcgcggcta cacgccttgc ccggaatgtc gcggcggaag
      481 attgcgccaa gccgcgcgcg acgtgaaaat cgccgaaaaa aatctgccgg aaatcgtcaa
      541 tctttccatc agcgacgccg gaaagttttt cgacgagctg gctttgagcg aagagcgcga
      601 aaaaatcgcc gaaaaacttc ttcttgaaat tcgccgccgg ctgaaatttc tggtcgatgt
      661 cggtctggat tatctgacgc tcgaccgtct ggcttcgact ttatcgggcg gcgaggcgca
      721 gcgcattcag ctcgccacaa atctcggctc gtcgctggtc ggcgcgctgt atgttctgga
      781 cgagccttca atcgggcttc acccgcgcga caacacgcgg ctggtcaatc ttttaaaaaa
      841 cctgcgcgac atcggcaaca cggttctggt cgtcgagcac gacgaggaaa tgatgcgcgc
      901 cgccgaccgc attctggaca tcggtttata cgcgggcgag ctcggcggaa acgtgatttt
      961 cgcaggcaat ttcgatgatt tactcgaaga cgaagaatct ctgacggcga aatacctgcg
     1021 cggcgaagcc gagataaaaa ttccgtcgca aagacgcaag ccgtcaaaac gaaaactcga
     1081 aatcgtcggc gcgcgcgagc acaatttaaa agccgtcgat gcggaaattc cgctcgatat
     1141 gctcgtctgc gtgacaggcg tttccggttc cggaaaatcg actctgattc acgacattat
     1201 ctacgccggg ttgaaaaaac agcgcggcga atggcaggga cacgtcgggc ttttcaagga
     1261 aatcaaaggc ggcgatttcg tcgacgacat catactggtc gaccagtcgc ccatcggcag
     1321 gacgccgcgc tcgaatccgg tcacgtacat caaggcttac gacgccatcc gcgaagtttt
     1381 ctcctctaca aatacggcga aaactcacgg ctacgacgct tcgcattttt cgtttaacgt
     1441 tccgggcgga cgctgcgaag tttgtcaggg cagcggcacg gtgacggtcg aaatgcagtt
     1501 tctcgccgac gtcgagctga cctgcgaaga atgttacggc acgcgtttta aagccgagat
     1561 tctcgacgtg cgctacaaag gcaaaaacat tcacgaagtc ctgcaaatga cgattcgcga
     1621 agccttgttt ttctttaagg aagtcaacaa gctcgtcaac aaattaaaag ttctggaagc
     1681 ggtaggcttg ggctacctgc ggctcggaca atcggcgacg accctgagcg gcggcgaagc
     1741 ccagcgcgtc aagctagccg cgcatctggc gcagaaaacg aaaaccaaaa ccttgtatat
     1801 cttcgacgag ccgaccaccg gcttgcattt cgacgacatc aacaaactgc tcgccgcttt
     1861 ccgcgctttg ctggataatg gcgcgagcct gctcgtcatc gaacacaatc tggacgtgat
     1921 aaaaacggct gattacgtca tagatttagg acccgaaggc ggcatcggcg gaggctgtat
     1981 cgtcaccacg ggaacgccgg aagaaatcgt aaaaagcgag aattcgcaca cgggcagata
     2041 tttgaaggat tatttgccgc agaattaaaa atccttgttg ggcttaag
//
LOCUS       DASWNE010000517         8828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_86934, whole genome shotgun sequence.
ACCESSION   DASWNE010000517 DASWNE010000000
VERSION     DASWNE010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8828
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86934"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..597
                     /locus_tag="VGB68_10230"
     CDS             112..597
                     /locus_tag="VGB68_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049653.1"
                     /translation="MKTLIESFDRQFLLLDARAREITGKTPSEKLYWQPRRREALFPV
                     NSCGEYILRSAGAVEQAFGGITTRLWDDPFEWTLPEELSTSDLILNYLREVEETRKRG
                     FSLFRSDADLSREIPAPEKLKTIFALLLETIARAENFQGRAIAVFREFSEAKIQTGWT
                     G"
     gene            758..1297
                     /locus_tag="VGB68_10235"
     CDS             758..1297
                     /locus_tag="VGB68_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049654.1"
                     /translation="MHCPRCGQQQVSEETRFCSRCGFPLGLVSEILAHGGVLPQLVDL
                     QRKKKKFWTRGNGVKVGLAWFLVLAVLITPVMGIFLEEEALVIAAFGFITGFLMMIFS
                     WMFLESEPKFSRIESGYQTADYLSGNAAPQNALPPQQAQPARDFVAPPAGAWKAPDTS
                     EMVPHSVTEGTTKLFRKDE"
     gene            1310..1861
                     /locus_tag="VGB68_10240"
     CDS             1310..1861
                     /locus_tag="VGB68_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049655.1"
                     /translation="MFCPTCGQRQATEETRFCSRCGFLLTGVTQLIFNGGALPQTLPP
                     AESKEMSPRKRGLRQGGMLFMSGILIVPLLAITLVGIFNFEGYAVGIAALITFLGGIL
                     RMIYALLFESSLPYGTTMEENVLHTAQNFLGKNKSAEALPPQQSIPTSTYVPPAAGNW
                     RDTNDLNAAPSVTENTTKFLDEN"
     gene            complement(1952..2743)
                     /locus_tag="VGB68_10245"
     CDS             complement(1952..2743)
                     /locus_tag="VGB68_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049656.1"
                     /translation="MSKTITFLLGALLGLVIGGAGIFYFYVGVPRAAAPPPGRPIQAP
                     DPSGAAAGTASVVLNQQFFDTVLGTIFRDMNAPSFPLQLTENSGGAESLQPEKIAFQS
                     GTCDGKITLKPEGSGVKTEVRLENSQILAPLAFSGSTTVLGQCIEFSGWAKANMALRF
                     DEAQQTVFGQINVETVNLDGLNPLVSGLVTPLVQNVLNQRVNPIVILRGQQIALNLPI
                     AATDGTLSARVKDVRSDIKDGALNLYITYDFQGTKGAASTQTPAL"
     gene            complement(2834..3769)
                     /locus_tag="VGB68_10250"
     CDS             complement(2834..3769)
                     /locus_tag="VGB68_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049657.1"
                     /translation="MKNLSFLLLSLAAALLTLGCGAPVSNGNSNTTISNSSNLNININ
                     SNANLTNTNANTAGGGGSGGSSSVETKEPDVYTATVTLKLETMGDQKVSVPSLQANVA
                     RNGEDRRMEFSLPGGEKLIYLDKGGKQFVISPNRRQYAELTKDALGFEVRRMLMPGEI
                     VNQVKNIRGVERVGEEQLSGRAVIKYRYGATANTGTQAGQVNTESFVYVDKETSLPLR
                     SETVSQSQGGNVQGVSGIRIGTEMSNITTSVDANLFAEPTDYKKVSPEEVKQQVNLVF
                     SAAAAIVGQLMRSAQPTNTGSPTTQAPPPTPAPTQ"
     gene            complement(3940..5673)
                     /gene="ggt"
                     /locus_tag="VGB68_10255"
     CDS             complement(3940..5673)
                     /gene="ggt"
                     /locus_tag="VGB68_10255"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY0049658.1"
                     /translation="MKSIKKLFVFCLIFSFFFVGLPRELTVRAAWREPVRGKRAMVAS
                     QSDLASKIGIDVISRGGNAVDAAVAVAIALAVTYPEAGNLGGGGFMLIRFRDGRSFAI
                     DYREMAPKAATRDVFVNKNGELIRGEGSSTIGYRASGVPGTPAGLELAFKKYGSGKIK
                     WADLIEPARKLAQDGFILSNRHANLFKSYENQLEKYADSKKIFLNGGKLFNEGDSLRQ
                     PELAQTLARIQRFGAKDFYEGETARLIAADMRANKGLITPEDLKNYRARERTPVTGTY
                     RGYEIITMPPPSSGGIVLLQALNMLEKYDVKKMNWASSEKYHLLTEVMRRAFADRAEY
                     MADPDFADVPTAGLIDKKYASMRSNSISMTRATPSSEIRAGQIAGTEPTETTHFTVVD
                     AQGNAVTNTYTINDLYGSAVTARGTGVLLNDEMDDFAARPGKPNMFGLVQGERNKVEP
                     GKRPLSSMTPTIVLRKDGSLWFALGARGGPRIITAVLQAVVNMIDHDMNIQQAIDAPR
                     VHHQWLPDEMLYEPVGMSSDTIKILENYGHKFAEKPANIASMTAIAIEEKTNVRLGAI
                     DSRSDGEAVGY"
     gene            6044..8647
                     /locus_tag="VGB68_10260"
     CDS             6044..8647
                     /locus_tag="VGB68_10260"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HEY0049659.1"
                     /translation="MKKIISTIIITVLCFSSVLFVPAQKSSGLAASSPISQGAKLFDS
                     TAAFTDGNGVWLEWRTGIESEIVGFQAYRIENGEKQLVSPVLIPGNFSTRGAKTSQGG
                     NYNYFDPQGSARSLYVIESIDVNGYKQTSREFYPQIVADLTALAGGSSEELQKRSAGS
                     NPSLQKDDLNLPTDLEAGAQTNNFVPADAAATQKWVAAQPGAKIAVKQDGLYRVTRAE
                     LQNGGFNVNAATNLWQLYADGVEQSINIGANRDYIEFYGRGVNSLESDSRIYYLVAGN
                     QTGRRMGTTFIRGISAKVVAPSFDQLAVKKDRLSYLSPVLNGTAENFFGSSISVGFPG
                     TTTINLSDLDSASVPVVTVSVGVQGYTFTPHQYKVQLNETEIGTLNGANRELVTGNFN
                     VPSSLLVEGANTVKITALSNSFAFTESVKISYPRRYQSIQERLLFYTQNYRATRVQRF
                     NTPDIRVFDLSDPTNPALVTELQISQQGLTYQVTLPSARSRVFYAVAGGGLLSAASIT
                     PNAPSTLSTAAHNANLIIISYRDWMTQAENWANYRRAPGMSVEVVNVEDVFDEFDFGQ
                     PSPEAIRGFLNYAKASWQTPPSYVLLLGDASYDARNFFGQGYNNFIPAQMVDTTFSTT
                     GSDDALADFNDDGLAEIAVGRIPARDAATVTTLLTKTAAFEQSLATAQSRGALCASDL
                     PDGFDFAAMCQRVLQPLPPTIPTMFVNRGEPDARATLMNSINSGKYIVNYSGHGTFGA
                     WAGNFFSRTDVPTMTNTNANQTIFTLLTCLNGYYVDAFSDGLAEVLVKKPEGGAVVAW
                     ASSGETTPDVQEIMATRFFQQIRNNASLNRIGDLANDAKTVISGGRDVRLSWVLIGDP
                     TMKVKPERKSE"
BASE COUNT         1964 a         2386 c         2348 g         2130 t
ORIGIN      
        1 tggcgtccga gccgcccgag tttcttcagc cctcgccgtc tgaaataacg ttttcggtcg
       61 gtcggcgttt aaatcgactg ccgccgcggg cgttcccgac ccggtttctt tatgaaaact
      121 ttaatcgaat ctttcgaccg gcagtttctt ttgcttgacg cccgcgcgcg ggaaattacg
      181 ggcaaaactc cgtccgagaa attatactgg cagccgcgac ggcgcgaagc gctgtttccg
      241 gtcaattcct gcggcgaata cattctgcgc tcggcgggcg cggtcgagca ggctttcggc
      301 ggcattacga cccgtctctg ggacgacccg tttgaatgga ctctgccgga agagctttcg
      361 acgagcgatt tgattttgaa ttatttacgc gaggtcgaag aaacccgaaa gcggggtttt
      421 tcgctgttcc ggtcggacgc ggatttaagc cgcgaaattc ccgcgcccga aaaattgaaa
      481 acgattttcg cgctgcttct ggagacaatc gcgcgggcgg agaattttca aggtcgcgcc
      541 atcgccgttt ttcgagagtt ttccgaagcg aaaattcaaa ccggatggac gggataaaag
      601 aaacgccgaa cgcggaaccc gtcaatgatg aacagggaac attaaagctg taatttatca
      661 ttcacgggtt ctttcgacgt tttggcaaat cgaacttttt tgtgcgaaaa ttcgttagcc
      721 gtaaagactt tcggaaattt tcaatctgga gaaaaaaatg cactgtcccc gctgcggtca
      781 acaacaggtt tccgaagaga cgcgcttttg ttcgcgctgc ggctttccgc tcggactggt
      841 ctcggaaatt ctcgctcacg gcggcgtctt gccgcagtta gtcgatttgc aaaggaaaaa
      901 gaaaaaattc tggacgcgcg gcaacggggt aaaagtcggc ttggcgtggt ttctggttct
      961 cgccgttttg ataactccgg tgatgggcat ctttctggag gaagaggcgt tggtaattgc
     1021 cgctttcggt tttatcaccg gatttctgat gatgattttt tcgtggatgt ttctggaaag
     1081 cgagccgaaa ttttcccgga ttgaatccgg ctatcaaacc gctgattatt taagcgggaa
     1141 cgccgcgccg caaaacgctc tgccgccaca gcaggcgcag cccgcgcggg atttcgtcgc
     1201 gccgcccgcc ggtgcgtgga aagcgccgga tacgagcgag atggttccgc acagcgtgac
     1261 ggaaggcacg accaagctgt ttcggaaaga cgagtaaaag gagaaaaaaa tgttttgtcc
     1321 aacctgcggt caacggcaag ccactgagga aacgcgtttc tgctcgcgtt gcgggttttt
     1381 gctgaccggc gtgacgcaat tgattttcaa cggcggcgcg ctgccgcaaa ctttgccgcc
     1441 ggctgaatcg aaggagatgt cgccgcgtaa aagaggcttg cggcagggcg gaatgctgtt
     1501 tatgtccggt attctgatag ttccgctgct ggcgattact ctggtcggaa tttttaattt
     1561 tgaaggctac gccgtcggga ttgccgcgct gataactttc ctcggcggaa ttttgcggat
     1621 gatttacgct ttgctgttcg aatcgagcct gccttacggg acgactatgg aggaaaacgt
     1681 tttacatacg gcgcagaatt ttctcggcaa aaacaaatca gccgaggcgc tgccgccgca
     1741 gcaatcgatt ccgacctcga cttacgtgcc gccggcggct ggcaactggc gcgacacgaa
     1801 cgatttaaac gccgcgccga gcgtcacgga aaacacgacg aaatttctgg acgaaaatta
     1861 agtaattaac gcaatacgtc gaaagcgcaa aatgtaggaa taattcttac attttgcgct
     1921 ttcgacgttt tgcgttttgg acgttttgct tttacagcgc gggcgtctgc gttgacgccg
     1981 ctcctttcgt gccctgaaaa tcgtaagtga tatacagatt caaagcgccg tctttaatgt
     2041 ccgagcggac gtctttgacg cgcgcgctca aagttccgtc ggtcgccgca atcggcagat
     2101 tcagagcgat ttgctgaccg cgcaggatga caatcggatt gacgcgctgg tttaaaacat
     2161 tctgcaccag cggcgtgacc aatccgctga cgagcgggtt caagccgtcg agattgaccg
     2221 tttcgacatt gatttgaccg aaaacggttt gctgcgcttc gtcaaaacgc agagccatat
     2281 tggctttcgc ccagccggaa aattcaatac actgacctaa aaccgtggtg ctgccgctaa
     2341 aggcgagcgg cgcgagaatc tgactgtttt ccagccggac ttcggttttg acgccgcttc
     2401 cctcgggctt aagcgtgatt ttcccgtcac aggttccgga ctgaaaagcg attttctccg
     2461 gctgcaagct ttccgctccg ccggaatttt ccgtgagctg cagcggaaaa gacggagcgt
     2521 tcatatcgcg gaaaatcgtg ccgagaaccg tatcgaaaaa ttgctggttc aggacgacgc
     2581 tcgccgtccc cgccgccgcg ccgcttgggt cgggcgcttg aatcggtctg ccgggcggcg
     2641 gggcggcggc tcgcggaacg ccgacgtaaa aataaaaaat tcccgctccg ccgattacta
     2701 aacccaacaa cgccccgagt aaaaaagtta tcgttttgct catataaacc ccttgaaaaa
     2761 tgccaggtgc aaaatgaaga agccggaaac cggaagataa atttccatcg tcttccggtt
     2821 tccgaccttt tctctattga gtcggcgccg gtgtcggcgg cggagcctga gtcgtcggac
     2881 tgcccgtgtt cgtcggctgt gccgaacgca tcagctgccc gacgattgcc gccgccgcgc
     2941 tgaacacgag attgacctgc tgtttgactt cttccggcga gactttttta taatcggtcg
     3001 gttcggcgaa aagattggca tcgacgctcg tcgtaatatt gctcatctcc gtgccgattc
     3061 tgattccgct tacgccctgc acgtttccgc cctgcgattg cgaaaccgtc tcggagcgaa
     3121 gcggcaggct cgtttcctta tcgacgtaaa cgaacgattc ggtgttgacc tgtccggctt
     3181 gcgttccggt gttggcggtc gcgccgtagc ggtatttgat gacggcgcga ccgctcaact
     3241 gttcttcgcc gacgcgctcg acgccgcgaa tatttttgac ctgattgacg atttcgccgg
     3301 gcatcagcat ccggcgcact tcaaaaccga gagcgtcttt cgtcaattcc gcgtattgcc
     3361 tgcggttcgg cgaaatgacg aattgttttc cgcctttatc cagatagata agcttttcgc
     3421 cgcccggcag agagaattcc atacggcggt cttcgccgtt gcgcgcgacg tttgcctgca
     3481 agctcggcac gctcactttc tggtcgccca tcgtctccag cttcagcgta acggtcgccg
     3541 tatacacatc cggctctttc gtctcgactg acgaactgcc gccgctgccg ccgccgcccg
     3601 cggtattggc gttggtgttc gtcaaattcg cgttcgaatt gatattgatg tttaaattgg
     3661 aactgtttga aatcgtcgtg ttggaattgc cgttcgacac cggagcgccg cagccgagcg
     3721 tcagcagcgc cgccgccagc gacaaaagca gaaaagacaa atttttcata aaccctcccg
     3781 ttttctatta ataatattta gaatagcgtt tttaccaacg tttcagcaag aagtttaggt
     3841 aagtggtaaa cggtaaacgg taaacggtaa gttcgttgtt ttgaaattgc atctttatct
     3901 attgcaactt accgtttacc gtttaccgct taccgttttt taatatccga ccgcttcgcc
     3961 gtcgctgcgc gaatcgatcg cgccgagccg gacattcgtt ttttcttcaa tcgcaatcgc
     4021 ggtcatcgag gcgatattgg cgggcttttc ggcgaattta tgcccgtaat tttccaggat
     4081 ttttatcgtg tccgagctca ttccgaccgg ctcgtagagc atctcgtcgg gcagccattg
     4141 atgatgaacg cgcggcgcgt cgatcgcctg ctggatgttc atatcgtggt ctatcatatt
     4201 aacgacggct tgcagaaccg ccgtgataat tcgcggtccg ccgcgcgcgc ccagggcgaa
     4261 ccacagcgag ccgtccttgc gcaaaacaat cgtcggcgtc atcgaagaaa gcggacgctt
     4321 gcccggctcg accttgttgc gctcgccctg caccaaaccg aacatattcg gcttgcccgg
     4381 tcgcgccgca aaatcgtcca tctcgtcgtt cagcaaaacg cccgtcccgc gagccgtcac
     4441 cgccgagccg tataaatcgt tgattgtata tgtgttggtc actgcgtttc cctgcgcgtc
     4501 gacgaccgta aagtgcgtcg tttcggtcgg ttcggttccc gcgatttgtc cggctcggat
     4561 ttcggagctc ggcgtcgccc tggtcatact tatcgaattc gaacgcatcg atgcgtattt
     4621 cttatcaatc aatccggcgg tcggaacgtc ggcgaaatcc gggtcagcca tatattcggc
     4681 gcggtcggca aaggcgcggc gcatcacctc ggtcaaaagg tgatattttt ccgacgaagc
     4741 ccagttcatt ttttttacgt cgtatttttc gagcatatta agggcttgca gcagaacgat
     4801 tccgcccgaa gacggcggcg gcatcgtgat aatctcgtag ccgcgatacg tgcctgtaac
     4861 gggcgtgcgc tctctggctc ggtaattttt taaatcttcc ggcgttatca agcctttatt
     4921 cgcccgcata tcggcggcaa tcaggcgcgc ggtctcgcct tcgtaaaaat ccttcgcgcc
     4981 gaatctttga attctcgcga gcgtctgcgc cagctccggc tgccgcaagc tgtcgccttc
     5041 attgaacaat ttcccgccgt tcagaaaaat ctttttgctg tcggcgtatt tttccagctg
     5101 gttttcgtag gatttgaaaa gatttgcgtg cctgttcgac agaatgaaac cgtcctgcgc
     5161 cagctttctg gcgggctcga ttaaatccgc ccattttatt ttgcccgagc cgtatttttt
     5221 aaaagccagc tccaagcccg ccggagtgcc gggaacgccc gaggcgcgat agccgatggt
     5281 cgacgagcct tcgcccctga taagctcgcc gtttttgttc acgaaaacat cgcgcgtcgc
     5341 cgctttcgga gccatctcgc ggtaatcaat cgcaaacgag cgcccgtcgc gaaaacgaat
     5401 cagcataaag ccgccgccgc ccaaatttcc ggcttccggg taagtcacgg ctaatgcaat
     5461 cgcgacggcg accgccgcat cgaccgcgtt gccgccgcga ctgataacgt cgatgccgat
     5521 ttttgacgcc aaatcgctct gcgaggcgac catcgcgcgc tttccgcgca caggctcgcg
     5581 ccaggcggct cgaaccgtaa gctcgcgcgg caagccgaca aaaaagaaac taaaaatcag
     5641 acagaaaacg aatagttttt ttattgattt cataactttg gtttttttga taatacaaca
     5701 aaattttacc agttcgggaa ttttatgcga aaggcggacg cttttttgcc gttacgaatg
     5761 acgaaacgac gaagccgaat aacgctcgga ttcgtaattt gtaacttagt cgttcataat
     5821 cgataaacaa atcgcgtttt atcaataaac aaatcgtgtt ttcggttttt ttaaagacta
     5881 aatcagattt aatctttaac aggttgtata gagtcttggc aaataatgta aaatgaagta
     5941 aattgaagtg tttcactctg gaaacatccc cacaatttac taaagtccgc ctcgaagctc
     6001 cgcaaccggg aaaatgcctg ttttggagca aggtcaaagt attatgaaaa agattatttc
     6061 aacaattatt atcaccgttc tttgtttttc gtccgtgctt ttcgttcccg cgcagaaaag
     6121 ctccggttta gccgcatcga gcccgatttc acaaggcgct aaattattcg attcgacggc
     6181 ggcttttacg gacggaaacg gcgtctggct cgaatggcgg acgggaatcg aaagcgagat
     6241 tgtgggattt caagcgtatc gcatcgaaaa cggcgaaaaa cagcttgtca gtcccgtttt
     6301 aattccgggc aatttctcga cgcgcggcgc gaaaacctcg cagggcggaa actacaatta
     6361 ctttgacccg caaggcagcg cgcgctctct gtatgttatc gaaagcatcg acgtcaacgg
     6421 atataaacaa acctcgcgtg aattttatcc gcaaatcgtt gcggatttga ccgcgctggc
     6481 gggcggctcg tccgaggaac tgcaaaagcg ttccgctggc tcgaacccga gccttcaaaa
     6541 agacgatttg aatctgccga ccgacctcga agccggagcg cagacaaata atttcgttcc
     6601 cgccgacgcc gcagcgaccc agaaatgggt ggcggcacag ccgggcgcga aaatcgccgt
     6661 caaacaggac ggtttatatc gcgtcacgcg cgccgagctg caaaacggcg gatttaacgt
     6721 caatgccgcg accaatctct ggcagcttta cgcggacggc gtcgagcaga gcatcaacat
     6781 cggcgccaac cgcgattaca tcgaatttta cggacgcgga gtaaattctc tggaaagcga
     6841 ctcgcgcatc tattatctgg tcgccggaaa tcaaaccggc agacgaatgg gaacgacctt
     6901 tattcgcggc atttcggcta aagtggtcgc gccgagcttt gaccagctcg ccgttaaaaa
     6961 agaccggctg tcttatctca gcccggtttt aaacggcacc gcggaaaact ttttcggctc
     7021 ttcgatcagc gtcggctttc ccggaaccac gactataaat ctttccgacc tcgattccgc
     7081 ttccgtgccg gtcgtgaccg taagcgtcgg agtgcaaggt tatacgttta cgccgcatca
     7141 atacaaggtt cagctcaacg agacggaaat cggcacgctg aacggagcaa accgcgaatt
     7201 ggtgaccggg aatttcaacg tgccgtcttc gctgctggtc gaaggggcga acaccgtaaa
     7261 aatcacggcg ctgtcgaact cgttcgcttt taccgaatcg gttaaaattt cctatccgcg
     7321 ccgctatcaa tcgattcagg agcgtttgtt gttttacacg caaaattatc gcgcgaccag
     7381 agtccagcgc tttaatacgc cggacatccg cgttttcgat ttgagcgacc cgaccaatcc
     7441 ggctctggta acggagctgc aaatttccca gcagggcttg acttatcagg tcactttgcc
     7501 gagcgctcgc agccgcgttt tttatgcggt ggcgggcggc gggcttttgt cggcggcatc
     7561 cataacgccg aatgcgcctt cgacgctttc gaccgccgcg cacaacgcta atttaatcat
     7621 catttcctac agggactgga tgacgcaagc cgagaattgg gcgaattatc gccgcgcgcc
     7681 gggcatgagc gtcgaggtcg ttaacgtcga ggacgtcttt gacgaattcg atttcgggca
     7741 gccgtcgcct gaagcgattc gcggttttct gaactacgcc aaagcaagct ggcagacgcc
     7801 gccgagctac gttcttttgc tcggcgacgc ctcctacgac gcgcgcaact tttttggtca
     7861 gggctacaat aattttattc cggcgcaaat ggtcgatacg actttttcga ccaccggcag
     7921 cgacgacgct ctcgccgatt tcaacgacga cgggttagcc gaaatcgccg tcggcagaat
     7981 cccggcgcgc gacgcggcaa ccgtgacgac gcttttaacc aaaacggcgg cgttcgagca
     8041 gagtctggcg acggcgcagt cgcgcggggc gctctgtgcc agcgatttgc cggacggttt
     8101 tgacttcgcc gcgatgtgcc agcgcgtatt gcagccgctg ccgccgacta ttccgacaat
     8161 gtttgtcaat cgcggcgagc cggacgcgcg cgccacgttg atgaactcga taaacagcgg
     8221 aaaatatatc gtcaattatt ccggtcacgg gacattcggc gcctgggcgg gaaatttctt
     8281 cagccgcaca gacgttccga cgatgacgaa cactaatgcc aaccagacaa tctttacgct
     8341 gctgacgtgt ttgaacggct actatgtcga cgctttcagc gacggtctcg ccgaagttct
     8401 ggtcaaaaag cccgaaggcg gcgcggttgt cgcctgggca tcgagcggag agacgacgcc
     8461 cgatgtgcag gaaattatgg cgacgcgctt tttccagcaa atccgaaaca acgcttcgct
     8521 gaatcggata ggcgatttgg ctaacgacgc gaaaaccgtg attagcggcg gacgcgacgt
     8581 ccgcctgtcg tgggttttga tcggcgaccc gacgatgaaa gtaaagccgg agagaaaaag
     8641 tgaataaagt gattaaggga ataggtgatt gggtttttat gcctaatcac ctattccctt
     8701 aatcacttta ttcactttct atttcggttg gattctcaat aaatagctgc acacggcaat
     8761 cggaatgccg ggcgccaccg taactctctg tcccatcgga acttcgtaat tgttgaccat
     8821 tgccagat
//
LOCUS       DASWNE010000518         1639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_87045, whole genome shotgun sequence.
ACCESSION   DASWNE010000518 DASWNE010000000
VERSION     DASWNE010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87045"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..838)
                     /locus_tag="VGB68_10265"
     CDS             complement(44..838)
                     /locus_tag="VGB68_10265"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0049660.1"
                     /translation="MLNGQKIIVVMPAYNAEKTLSQTVAEIPRDIVDEILLVDDASTD
                     KTVLKSCELELITFQHQRNFGYGRNQKTCYREALSRGADIVVMVHPDYQYSPHLIVPI
                     SAMIAYGEYDVVLASRILGKGAVAGGMPVYKYVANRFLTLAQNLLMSQKLSEYHTGFR
                     AFRREVLENLPLEANSDDFVFDNEMIAQAFYFGYRVGEISCPTRYFEDASSINFSRSV
                     KYGLGVLATSLKFRLHRSGIFKNKLFRERGQKALPEYYRKIESERR"
     gene            complement(912..>1639)
                     /locus_tag="VGB68_10270"
     CDS             complement(912..>1639)
                     /locus_tag="VGB68_10270"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0049661.1"
                     /translation="LEKSFDSGKKAKRLLDVGCGEGTFLEAAKKRGWQAVGTEAVAEN
                     FRDSDLEVFSDFAEVKAKYGAESFDAITMWHTLEHFQMPREVLRQVFELLAPKGVLLV
                     AVPDARGWQALASGKYWLHLDVPRHLFHFGFGSLDLLLRQSGFRIKSHRHQEFEYDLL
                     GWSQSGLNRIFTAPNVFFKTLSGHRTNVSRAARAANFALGVIFSAVSLPLVWLGTICG
                     KGGTLVVGAIKAEPIELSAEREK"
BASE COUNT          385 a          450 c          423 g          381 t
ORIGIN      
        1 tttttcgcgt ctcgcttttc gttttttcac gtctcgcttt tcgctagcgt ctttcggact
       61 cgattttccg gtaatattca ggcagcgcct tttgcccgcg ctcgcggaaa agtttgtttt
      121 taaatattcc cgagcgatgc agtcgaaact tcagagaagt cgccagaacg ccgagaccgt
      181 atttcacgct ccgcgaaaaa tttatcgaag acgcatcttc gaaatagcgt gtcgggcagg
      241 aaatttcgcc gacgcggtag ccgaaataga aggcttgcgc aatcatctcg ttgtcgaata
      301 caaaatcgtc ggagttagct tcgagcggca gattttccag cacctcgcgg cgaaaagcgc
      361 gaaagcccgt gtgatattcg ctcaatttct ggctcatcag caggttctgc gcgagcgtca
      421 aaaaacgatt ggcgacgtat ttgtaaaccg gcattccgcc cgcgacggcg cctttgccca
      481 aaatgcgcga agccagcacg acatcgtatt cgccgtaggc aatcatcgcg gaaatcggca
      541 caatcaggtg cggcgagtat tgataatccg ggtgtaccat cacgacaata tccgcgcctc
      601 tggagagcgc ctcgcggtaa caggttttct gattacgccc gtagccgaaa tttctctggt
      661 gctgaaacgt gattagctca agctcacagg atttcaaaac cgttttgtcc gtgctggcgt
      721 cgtcgaccag cagaatctcg tccacgatgt cgcgcggaat ttcggcgacc gtctgcgata
      781 aagttttttc cgcgttgtaa gcgggcatta cgacgatgat tttttgaccg ttgagcattt
      841 tagcaaaagt aagaaattag tccacagatt aacacaagtc agcgccggta gggaaaaatg
      901 taaaatccgc attatttctc ccgttcggct gacaattcta tcggttcggc tttgattgcg
      961 ccgacgacca gcgttccgcc ttttccgcaa attgttccga gccagacgag cggcagagaa
     1021 acggcggaaa agattacgcc gagcgcaaaa ttggcggcgc gggctgcgcg gctgacgttt
     1081 gtccggtgtc ccgaaagagt tttaaaaaaa acgttcggcg cggtgaaaat ccggttcaaa
     1141 ccgctctgcg accagccgag caaatcgtat tcaaactcct gatgccggtg gcttttaatc
     1201 cgaaaaccgc tttgccgcag aagcaaatcc aaagacccga atccgaaatg aaaaagatgc
     1261 cgcggcacgt ccagatgcag ccagtatttg ccggaggcga gcgcctgcca gccgcgggcg
     1321 tccggcacgg cgacgagcaa aacgcctttc ggcgcgagca gctcaaagac ttgccgcaga
     1381 acttcgcgcg gcatttggaa atgttcgagc gtatgccaca tcgttatcgc gtcgaaagat
     1441 tccgctccgt attttgcctt gacctcggcg aaatcggaaa aaacctccaa atccgagtca
     1501 cggaaatttt cggcgaccgc ctcggttccg accgcttgcc agccgcgctt tttcgccgcc
     1561 tccagaaaag tgccttcgcc gcagccgaca tcgagcaggc gcttcgcttt tttgccggaa
     1621 tcgaagcttt tttccagcc
//
LOCUS       DASWNE010000519         9956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_194700, whole genome shotgun sequence.
ACCESSION   DASWNE010000519 DASWNE010000000
VERSION     DASWNE010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9956
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194700"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1695)
                     /locus_tag="VGB68_10275"
     CDS             complement(<1..1695)
                     /locus_tag="VGB68_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049662.1"
                     /translation="MNPNEIWQVDVGGQVYEASFAELTQWVTEGSLLPQDKVRRGNLR
                     WLDAIKVPALYGFFNAKELGIAPPVLPVMISTDTEAAAPASETAQMQSGSFAPAATVS
                     HNQFNLQNQFASPNQFNPTNPSPAPTQFDAQSPFDAPADFAPTEAFQQPYQTTPQNFQ
                     ADFTQPMPQASACLVHPENEPAFYCDGCANLFCKGCPTSYGGTVKICPMCGAMCKPLE
                     AVREQQKKAVQFHQDLAQGFGFEDFGKALAYPFKFKFSLAAGAIMFMLFTLGQSSAGV
                     GGIWMMSASGFCLMLSNMLTFGVLANTVENMSQGKLSENFMPGFDDFNILDDVLHPFF
                     LSIGAYLVSFGLLFVILIGGLWYMSNRAVESHGDKVADMSKIIQENREAKDEGRILPD
                     GTVIPPFDQLTPAQKQALDDNDIEQLQEMLVEHRKANLESVVGKTAETKDREFSQMLS
                     QLASIGTPVMLLAFLAFLWGIFYLPAACAVAGYTRSFAATVNPMVGLDTIKRMGFDYV
                     KILLMVFILFVASSIIGGIFGFLLKAFDMPGVGNLPANAVVSLFTFYFSVVFSLLIG"
     gene            complement(1823..3628)
                     /locus_tag="VGB68_10280"
     CDS             complement(1823..3628)
                     /locus_tag="VGB68_10280"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY0049663.1"
                     /translation="MPRLKRKISLVYFVLITLLLVGLVPLVVTGWLLSERSARELRAV
                     EGRYQTQLVQDKARQIEMLGQRYGDLVDGFAKAFELSGDFSLLSSSAAQKKLGQTLQE
                     NPHLLSLYVKPVSGEPLSVYRAEAINAPEIEKLSSNVLARLGEKELIMGQPQKLQTSQ
                     QLVLTIAAPVMNENQITAAVVAIVSLREISRIMSESGGASEKELWEAGLPIVFVVDEQ
                     GRAIFHPDSQVVTNQKPQTNLKIVQEWQEASRQIQSALVPFAAEFENQKHEMIGAYST
                     ANFNNELAFGVIAMQDENTALASVGEMRRQTWITSLALAVLALIAGSLFARQLTSPIL
                     ELVSAAKRIADGDLSTRIETKSIKEVETLGTAFNLMSDRLEEHIADLAKAAQENRELF
                     VGTVKALAAAIDGKDKYTRGHSERVARFSVAVGKRMGMGETELETLRISALLHDVGKI
                     AIDDNILKKPAALTNDEFEIMKTHPQRGFKIMSQIPAMKEFLPGMYMHHEMVNGEGYP
                     QGLRDDQIPLQAKIVSVADTFDAMTTDRPYQKGMNLDDALNRIRSFVGTRYDGKVVEA
                     LVQACAEGQIRPVGGAVVKPQNSAQNNNAAPLKVA"
     gene            4005..4583
                     /locus_tag="VGB68_10285"
     CDS             4005..4583
                     /locus_tag="VGB68_10285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0049664.1"
                     /translation="MTANLTDEQLVEIAIKENPDAFGEIVNRWERKIFALCFGMLSRE
                     EDARDAAQETFIAAYRNLSNFRGEAKVSSWLHRIAVNQCLTKQRRAKVRSEIYLEDEA
                     ETNQMVFVAASGASPARKTEQSERLQFVRQAVNSLPPDLRQVIVMKEFEEMTFQQISE
                     ALDLPLSTVKSRLYTALKQLRMKLERTPVEVI"
     gene            4707..5498
                     /locus_tag="VGB68_10290"
     CDS             4707..5498
                     /locus_tag="VGB68_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY0049665.1"
                     /translation="MNSSSSNHNSSCNFSDLLVSYLYGEIGESEKFRFESHVSSCGVC
                     ADEIAAFGGVRASVQNWRETEFAALSTPVFELPFEKEKTKILSMETTAASRSWLAGLR
                     EMFSISPVLTGAATACAALAICAGLFYVALSSSMQTDRSLAGTNKNASVAIVPSPTIE
                     STITAGNAESGDKQPDETSKPEISKDKKQIPSKTVPAAERAAVNENTSVKPLKTNVAP
                     KPSSKEKPASSAKKPSKQSDIEFSTREEEDKSLRLADLFDEVSMK"
     gene            5515..6066
                     /locus_tag="VGB68_10295"
     CDS             5515..6066
                     /locus_tag="VGB68_10295"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HEY0049666.1"
                     /translation="MRKFRLFSLIITLFVFGFAAETKAQDEMPPDEQPPMQKRQRPRP
                     NLLQELGLSREQIQQIRRINQARRPLMQEAQRRFREANRALDEAIYADSPNDSDVQAR
                     MKDVQLAQAEIIKIRTTSEIAIRKVLTPEQLVKFRELRENFMRRMQNQQNPPNQTDAP
                     NQREEIDNPNRRNLNRLRRRRGF"
     gene            6268..7035
                     /locus_tag="VGB68_10300"
     CDS             6268..7035
                     /locus_tag="VGB68_10300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0049667.1"
                     /translation="MNPLKNFLLQLQEITNLIIAAAIGLRKRPRYYREIIKQMDSIGV
                     GSLPIVILTGFFTGAVLVLQSFSILAYYGAQGQTGRMVSFSLVRELGPVLSALMVAGR
                     IGSSISAELGSMVVSQQIDAMRALGTDPNKKLVAPRIIALILMLPLLTIAADIFGIIG
                     GGFIANQLFSLDYTLFITSVREGVTVKNIIVGVVKPLFFGLIIGAVACYKGLNTTGGT
                     VGVGQSTTKAVVLASIVSIIVDFFLTRGLQNMLGIEF"
     gene            7106..8017
                     /locus_tag="VGB68_10305"
     CDS             7106..8017
                     /locus_tag="VGB68_10305"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0049668.1"
                     /translation="MSSAEENKEVFAENQFVEEELEPVSLEDATYEPERMIPAIEFRD
                     VHLSFDDQKILNGVSFTVRKGETKIVLGRSGGGKSTTIRLVLGLLKPDSGQILVDGED
                     ITNYDEIEMMRVRQKIGMVFQEGALFDSLTVYDNVAYRLHEQRLPEAQVEQEVRRMLH
                     FVDLEEAIDKMPSELSGGMRRRVGIARALVGDPKIILFDEPTAGLDPPTARTICELAI
                     KLRDLEDVSSIFVTHEMQNLEYLCTEYATVNESGEVVFQEEGDTLCLLNTEVIMFREG
                     KIIFSGKDEELRASDDQYVRRFLRGKS"
     gene            8061..8357
                     /locus_tag="VGB68_10310"
     CDS             8061..8357
                     /locus_tag="VGB68_10310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEY0049669.1"
                     /translation="MSGNILKDKSYKFALRIIKLYKHLTEDKKEFVLSKRVLRSETWM
                     GANIIEGNHAESKMDFVHKLAVAHKESFETEYRINLLKDSEYITEKQAESVLND"
     gene            8643..9848
                     /locus_tag="VGB68_10315"
     CDS             8643..9848
                     /locus_tag="VGB68_10315"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEY0049670.1"
                     /translation="MPPTTRNIGLSQLRVGIFVLIALALLAFLIINSSGEFNPFERRM
                     RLKAHFASADGLREGAEVQLAGVHIGQVEQVRFLPPDSEDRARIEAIMSVSEQLDGRP
                     VTERIRTDSTAQLIATSVLANDKMINITPGSARGGAVEENHVLDSSTSISINQLTQTG
                     NELLTQINRLAEPTNQILNKANRGEGTLGRIINDDALYNNLDSTVGETRATMLKLQNT
                     IDRINRGEGSAGKLLNDPELYNSLNRTTTQLEAIARDLRAGRGSAGKFLSDDELYNEA
                     RATILEVRASVNRLTQVADTFDTIARDLNEGKGTAGKFLKDEQLYNDARETLAKFNST
                     ATKLDIMLADVQSGKGTVGKLLTDETLYNNANQTMSNVNQLSSEGTKLIYDFRQNPKK
                     YLTIRFRLF"
BASE COUNT         2483 a         2525 c         2596 g         2352 t
ORIGIN      
        1 gccgataagg agcgagaaga cgaccgagaa atagaatgta aacaggctga cgacggcgtt
       61 ggcgggcaga tttccgacgc ccggcatatc gaaggctttt aaaagaaagc cgaaaattcc
      121 gccgataatg gacgaggcga cgaacaatat gaaaaccatc aataatattt tcacatagtc
      181 aaagcccatc cgtttaatcg tatccagacc gaccatcgga ttgacggtcg cggcaaacga
      241 gcgcgtgtag cccgcgacgg cgcacgccgc cggaagataa aatattcccc acaaaaaagc
      301 caggaaagcc agcagcataa ccggcgttcc gatggaggca agctgagaaa gcatctggct
      361 gaactctctg tctttcgtct ccgccgtttt cccgacgacc gattcgagat tggctttgcg
      421 gtgctcgacc agcatctcct gcaattgttc gatgtcgttg tcgtcgagcg cctgtttctg
      481 cgccggagtc agctggtcaa acggcggaat caccgtgccg tccggcagaa ttctgccttc
      541 gtccttggct tcgcggtttt cctgaattat tttcgacata tcggcaacct tatcgccgtg
      601 gctttcgacc gctctgttcg acatatacca aagcccgccg ataagtatca caaacagtaa
      661 gccgaacgaa acgagataag cgccgatgct caggaaaaac ggatgcaaaa cgtcgtccag
      721 aatattgaaa tcgtcaaagc ccggcataaa gttttcgctc agcttgccct gcgacatatt
      781 ctcgaccgtg tttgccagaa cgccgaaagt gagcatattc gaaagcatca gacaaaaacc
      841 cgacgcagac atcatccaga tgccgccgac gcccgccgag ctttgcccga gcgtgaaaag
      901 cataaacatt atcgcgcccg ccgccaggct gaatttgaat ttgaacggat aagccagagc
      961 cttgccgaaa tcttcgaaac cgaaaccctg cgccaggtcc tgatgaaact gaaccgcctt
     1021 tttctgctgc tcgcgcaccg cttccagagg cttgcacatc gcgccgcaca tcgggcaaat
     1081 cttgaccgtg ccgccgtaag aagtcgggca gcccttgcag aaaagattgg cgcagccgtc
     1141 gcaatagaaa gccggttcgt tttcgggatg aacgagacac gcgcttgcct gcggcatcgg
     1201 ctgcgtgaaa tcagcctgaa aattctgcgg cgtggtctga tacggctgct gaaaagcttc
     1261 ggtcggcgcg aaatcagcgg gcgcgtcaaa cgggctttgc gcgtcgaatt gtgtcggcgc
     1321 gggcgacgga ttcgtcggat taaattgatt cggcgacgcg aattgatttt gtaaattaaa
     1381 ttgattgtgc gaaacggtcg ccgcgggcgc aaagcttccg ctctgcatct gcgcggtttc
     1441 ggaagcgggc gcggcggctt ccgtgtcggt cgaaatcata accggcagca cgggcggcgc
     1501 gatgccgagc tccttcgcgt tgaaaaaccc gtaaagcgcc ggaactttta tcgcgtcgag
     1561 ccagcgcaga ttgcctcggc gcactttgtc ctgaggcagc aaagagcctt cggtcaccca
     1621 ttgcgtcaat tccgcaaagc ttgcttcgta aacttgtccg ccaacatcta cttgccaaat
     1681 ttcattcgga ttcatcggtg tatcgttcct ttaaaattat tcgggattca ggattcagga
     1741 ttcgggattc aggattcagg attcaggatt gaaagaaaat tcctaatcct gaatcctgaa
     1801 tcctgaatcc tgaatgtgga tttcaagcga ctttgagcgg tgcggcgttg ttgttttgag
     1861 ccgagttctg aggtttgacg accgcgccgc cgacaggtcg aatttgccct tcggcgcagg
     1921 cttgaaccaa tgcttcgacc acttttccgt cgtagcgcgt gccgacaaag ctccggatgc
     1981 ggttcagcgc gtcgtccaga ttcattccct tctgataagg tcggtcggtc gtcatcgcgt
     2041 caaacgtgtc ggcgaccgag acgattttcg cctgtagcgg aatctggtcg tcgcgcaagc
     2101 cctgcggata gccttcgccg ttgaccatct cgtgatgcat atacattccg ggcaaaaatt
     2161 ccttcatcgc cggtatctgc gacataatct tgaaacctct ctgcgggtga gtcttcatga
     2221 tttcaaattc gtcgttcgtc agggcggctg gctttttcag aatattgtcg tcgatggcga
     2281 ttttgccgac gtcgtgcagc agggcgctga tgcgcagggt ttccagctcg gtttcgccca
     2341 ttcccatacg ttttccgacg gcgacggaaa atcgcgcgac tctctccgaa tgaccgcgcg
     2401 tgtatttgtc tttgccgtcg atggcggcgg cgagcgcttt gaccgtgccg acaaaaagct
     2461 cgcggttttc ctgcgccgcc tttgccaggt cggcgatgtg ctcttcgagc cggtcgctca
     2521 tcaaattaaa ggcggtgccc agtgtttcga cttctttaat gcttttcgtt tcgatgcgcg
     2581 tggaaaggtc gccgtcggcg atgcgctttg ccgccgagac gagttcgaga atcgggctcg
     2641 tcagctgcct cgcaaataag cttccggcaa tcagcgccag caccgccagc gccagactcg
     2701 ttatccacgt ctgtcggcgc atctcgccga ccgaggcgag cgccgtgttt tcgtcctgca
     2761 tcgcaatcac gccgaaagcc agttcgttgt taaaattagc ggtcgaatac gcgccgatca
     2821 tctcgtgctt ttgattttca aattccgccg caaacggcac gagcgccgac tgaatctggc
     2881 gactggcttc ctgccattcc tgcacgattt tcagattcgt ctgcggtttc tggttggtta
     2941 cgacctgcga atcgggatga aaaatcgcgc gtccctgctc gtcgacgacg aaaacaatcg
     3001 gcaagcccgc ctcccacaat tctttttcgc tcgcgccgcc gctctccgac ataatgcgcg
     3061 agatttcgcg taatgaaacg atggcgacga ccgccgccgt gatttgattt tcgttcatca
     3121 cgggcgcggc aatcgtcagg acgagctgct ggctggtttg cagtttctgc ggctgtccca
     3181 taattaactc cttctcgccc agacgcgcca gaacgtttga agagagcttt tcgatttccg
     3241 gcgcgttgat ggcttcggcg cggtagaccg aaagcggctc gccgctgacg ggttttacat
     3301 acagcgaaag cagatgcgga ttttcctgca aggtctgtcc cagctttttc tgggcggcgg
     3361 atgaagaaag cagagaaaaa tcgccggaca gctcgaaggc tttggcaaag ccgtcgacca
     3421 gatcgccgta acgctgtccg agcatttcaa tctgccgcgc cttgtcctgc actaattgtg
     3481 tctggtatcg accttcgacg gcgcgcaatt cgcgcgcgct tctttcggac aaaagccagc
     3541 ccgtcacgac cagcggaacc agcccgacca gcagaagcgt gatgagcaca aaataaacca
     3601 gactgatttt tctttttagc cgaggcattc tcgttttgca ggcagtatcg acaggcagga
     3661 caatcgcctt agaaatttta tgatatattg tttattatcg tcaacatttt ttaatcagtt
     3721 ttaacaattc gtcgaaatat aaatcacgag cgtctacggt ttcaagcgtt ttaccttaaa
     3781 gatttataac ttcagttttc tgccgaaaag acaaaaatat ttaactcgaa atcacagact
     3841 ccggcgtcga gcaggcggct ggcgcaggcg gtttcggctg taaaattttt ccgactttcg
     3901 gcgaattact tgcgtctaaa ggtttgagtg tgaaaaactt ttcacattcg cgggcgaagt
     3961 tttgaaaatt cgcgtgcgcg ggtctgacag cggcgtcgag ggcaatgaca gcgaatttaa
     4021 cggatgagca gttggtcgaa attgcaataa aggaaaatcc tgatgctttc ggcgaaatcg
     4081 tgaatagatg ggagcgaaag attttcgcgc tctgtttcgg tatgctcagc cgcgaagaag
     4141 acgcgcgcga cgccgcgcag gaaactttta tcgccgctta tcggaatctg tcgaattttc
     4201 gcggcgaggc gaaagtttcc tcgtggctgc atcgcatcgc ggtcaatcaa tgtttgacaa
     4261 aacagcgccg cgccaaggtt cgctcagaaa tttatctgga agacgaagct gaaaccaacc
     4321 agatggtttt cgtcgcggca tcgggcgcat cgcccgcgcg caagaccgag cagagcgaaa
     4381 gacttcagtt tgtcaggcag gcggttaatt cccttccgcc ggatttgcgg caggtcatcg
     4441 tgatgaagga atttgaggaa atgacgtttc agcagatttc ggaggcgctc gatttgccct
     4501 tgagcacggt caaaagccgg ctttacacgg cgctcaaaca gcttcggatg aagcttgaaa
     4561 gaacgccggt ggaagtgatt taaaaagtgt ggaagcggtt taaaaagtaa ggaacgggac
     4621 gctgattttt aagaattttt aagatttatc gaggtaaata aaatcctaat caaacttaaa
     4681 aaatcactgt tccgacagaa aaaaaaatga acagcagcag cagcaatcac aattcgtctt
     4741 gtaatttttc cgaccttctg gtctcgtatc tatacggcga aatcggcgaa tcggaaaaat
     4801 ttcgttttga atcgcacgtt tcgagttgcg gcgtctgcgc cgacgaaatc gcggcgttcg
     4861 gcggcgttcg cgcatcggtg caaaattggc gcgagacgga atttgcagcg ctttcgacgc
     4921 cggttttcga attgcctttc gaaaaggaaa aaacaaagat tttatcgatg gaaacgacgg
     4981 cggcttcacg ctcgtggctg gctggtctgc gcgaaatgtt ttcaatctct cccgtgttga
     5041 ccggcgcggc gacggcgtgc gcggctttgg cgatttgcgc cggtttgttt tatgtcgcgc
     5101 tcagctcttc catgcaaacc gacaggagcc tggcgggaac aaataaaaat gcttccgttg
     5161 caatcgttcc ttcgccgaca atcgaatcga cgattacggc gggtaatgcg gaaagcggcg
     5221 acaaacaacc ggacgaaacg tcaaagcctg aaatttccaa agataaaaag caaattccgt
     5281 cgaaaaccgt gcccgcagcg gaaagagctg ccgtcaacga aaatacgagc gtgaaaccgc
     5341 ttaaaacaaa cgtcgcgccg aaaccgtcga gtaaagaaaa gcccgcatcg agcgcgaaaa
     5401 agccgtcaaa gcaatcggac atagagtttt cgacgcgcga ggaagaagat aaatctctgc
     5461 gtctggcgga cttgttcgac gaagtcagta tgaaataatt ttttggagaa acgaatgaga
     5521 aaattcagac ttttcagctt aattatcacg cttttcgttt tcggcttcgc cgccgaaacg
     5581 aaagcgcagg acgaaatgcc gcccgacgag cagccgccga tgcagaagcg acagcgcccg
     5641 cgaccgaatc ttttgcagga gctggggctt tcgcgcgagc agattcagca aatccggcgc
     5701 attaaccaag cccggagacc tctgatgcag gaagcgcagc ggcgctttcg cgaagcgaat
     5761 cgcgctctgg acgaagcgat ttacgccgat tcgccgaatg attccgacgt tcaggcgcga
     5821 atgaaagacg ttcagctcgc gcaggcggaa ataatcaaaa tccgcaccac gtccgagatt
     5881 gccatccgca aggttttgac gccggaacag ctcgtcaaat tccgcgaact gcgcgaaaat
     5941 tttatgcgcc ggatgcagaa tcaacaaaat ccgccgaatc agacggacgc gccgaatcag
     6001 cgcgaggaaa tcgataatcc gaatcgacgg aatttaaatc gcctgcgccg gcggcgcggc
     6061 ttttaaattt ctcgattttt tattttctca aacgccccgg cggtttattc tccgattttt
     6121 agccgaatcc tgaaaaggtt cggctattat tttgtctttt cggcgcggtt cgttgtaatc
     6181 tttagcagtt attattttat tagccgtcag ccgccggcaa tcggcagcca gccgaaagcc
     6241 ggacactgac cgaagattac tgaccgaatg aatccgctca aaaattttct gctgcaattg
     6301 caggaaatta ccaatctgat tattgccgcc gcaatcggac ttcgcaagcg tccgcgttat
     6361 tatcgtgaaa tcatcaaaca gatggacagc atcggcgtcg gctcgctgcc gatcgttatc
     6421 ctgaccggat tttttaccgg cgctgtgctg gttctgcaaa gcttttcgat tctggcttat
     6481 tacggcgcgc agggtcagac cggaaggatg gtttcctttt cgctggttcg cgaattgggt
     6541 ccggttttgt cggcgctgat ggtcgccggt cgcatcggct cgtcaatctc ggcggagctc
     6601 ggctcgatgg tcgtctcgca gcaaatcgac gcgatgcgag cactcggcac cgacccgaat
     6661 aaaaaactgg tcgcgccccg gattatcgct ttgattctga tgctgccgct tttgacgatt
     6721 gcggctgata ttttcggaat tatcggcggc ggctttatcg ccaaccagct tttcagcctg
     6781 gattacacgc ttttcatcac gtccgttcgc gagggcgtga cggtcaaaaa catcatcgtc
     6841 ggcgtcgtga agccgctgtt tttcgggtta attatcggcg cggtcgcctg ctataaaggc
     6901 ttgaatacga ccggcggcac ggtcggcgtc gggcaatcga cgacgaaagc cgtcgttctg
     6961 gcttcgattg tctctatcat cgtcgatttt ttcctgacgc gcggtttgca gaatatgctg
     7021 ggtatcgagt tttaatttaa ttacgagctt taaatgaatc gctaaataac aaattcagat
     7081 tcaaaattca aaactcaaat tttttatgtc gtcagccgaa gaaaataaag aagtatttgc
     7141 ggaaaaccaa ttcgtcgaag aagagcttga gccggtctcg ctcgaagacg cgacctatga
     7201 gccggagcgg atgattcccg ccatcgaatt tcgagacgtt catctttcct tcgacgacca
     7261 gaaaatatta aacggcgtca gctttacggt caggaaaggc gagacgaaaa tcgttctcgg
     7321 gcgctcgggc ggcggaaaat cgacgacgat tcggctggtt ctcggtttgc tgaaaccgga
     7381 ttcgggtcag attctggttg acggcgagga cattaccaat tacgacgaaa tcgagatgat
     7441 gcgcgtgcgc cagaaaatcg ggatggtttt tcaggaaggc gcgctgtttg attcgctgac
     7501 ggtttacgac aacgtcgcgt atagactgca cgagcagcgt ctgccggaag cgcaggtcga
     7561 gcaggaagtg cgccggatgc tccacttcgt cgacctcgaa gaggcaatcg ataaaatgcc
     7621 gagcgagctt tccggcggaa tgcgccgccg cgtcggcatc gcgcgcgctc tggtcggcga
     7681 cccgaagatt attttatttg acgagccgac cgccgggctc gacccgccga cggcgcgcac
     7741 gatttgcgag ctggcgataa aattgcgcga tttggaagac gtttcctcga ttttcgtgac
     7801 gcacgagatg cagaatctcg aatatctctg caccgaatac gcgaccgtca acgaatcggg
     7861 cgaagtcgtt tttcaggaag aaggcgacac gctttgtctt ttgaacacgg aagttataat
     7921 gtttcgggaa ggcaagataa ttttcagcgg caaggacgaa gagcttcgcg ccagcgacga
     7981 ccagtatgtc cgccgctttt tgcggggaaa gagttaatgc aaaacgtcga aaatcaaaaa
     8041 cttaaaatgc agaaggcaaa atgagcggga atattttaaa agacaaatct tataaatttg
     8101 ctttgcgcat tattaagctt tataagcatc taacggaaga taagaaagag tttgttttat
     8161 cgaaacgggt tttgcgttcg gaaacttgga tgggcgcgaa tattattgaa ggaaatcacg
     8221 ccgaatcgaa aatggatttt gttcataagc tcgcggtcgc gcataaagaa tcttttgaaa
     8281 cggaatatcg gataaattta ttaaaagaca gcgagtatat tactgaaaaa caagctgaat
     8341 cggttttgaa cgattgacgg gaactgcaaa atctctaaac aacatcaatc aaaactgcgt
     8401 aaaacaacca gtaacggctt tgcgcaaaaa taaagaaagg gaaaattgta aattgtaaat
     8461 tgtaaattct aaattgatgt tcggtatctt ttgtgaagta attgagaatt aataatttac
     8521 aatttacaat tagccatcgg aatttttctc gattttattt atttttgtgc aaagcaacca
     8581 gtaaccactt tgcattttaa gttttgcatt ttcgacgttt tgcattcagc atttttaaac
     8641 ttatgccgcc aactaccaga aacatcggat tgagccagct gcgcgtcggg attttcgtcc
     8701 ttatagcgct tgcgctgctc gcttttttga ttatcaattc ttcgggcgaa tttaatccgt
     8761 tcgagcgaag aatgcgtctg aaagcgcact ttgccagcgc cgacggcttg cgcgagggcg
     8821 cggaagtgca gctcgccgga gttcacatcg ggcaggtcga acaggttcgc tttctgccgc
     8881 cggacagcga agacagggcg cgcatcgaag cgattatgag cgtctccgaa cagctcgacg
     8941 gcagacccgt caccgagcgc atccgaaccg actcgaccgc gcagctgatt gccacatccg
     9001 ttttggcaaa cgataaaatg attaacatca cgcccggttc ggcaagaggc ggagccgtcg
     9061 aggaaaacca cgtgctcgat tccagcacct cgatttcgat caatcagctg acgcagaccg
     9121 gcaacgagct gctgactcaa atcaaccggc tcgccgagcc gaccaaccaa attctgaaca
     9181 aagccaatcg cggcgaaggc acgctcggac gcattatcaa tgacgatgcg ctttacaata
     9241 atctggattc gaccgtcggc gaaacccgcg cgacgatgct caaactgcaa aacacgattg
     9301 accgcatcaa tcgcggcgaa ggcagcgccg gaaagctttt aaacgacccg gagctttaca
     9361 acagcctgaa ccgaaccacc acgcagctcg aagcgatagc ccgcgatttg cgcgccgggc
     9421 gcggctctgc cggaaagttt ttatccgacg acgagcttta taatgaagcc cgcgcgacga
     9481 ttctggaagt ccgcgcttcg gtcaaccgcc tgacgcaggt cgccgatacc ttcgatacga
     9541 ttgcgcgaga tttaaacgaa ggcaaaggca cagccggaaa attcctgaaa gacgagcagc
     9601 tctacaacga cgcccgcgaa acgctggcaa aatttaattc gaccgcgacg aagctggaca
     9661 ttatgctcgc cgacgttcaa agcggaaaag gcaccgtcgg caagcttctg accgacgaaa
     9721 cgctctacaa caacgccaat caaacaatgt cgaacgtcaa tcaattatct tcggaaggca
     9781 cgaaattaat ttacgacttc cgccagaatc cgaaaaaata tctgaccata agattcagac
     9841 tgttttgata attttttaat tgattgtctg taaaaaagtt tttaggtctg taataaagtt
     9901 tttaggtgga gggctttttt tcagacttgt ttgtaataaa gtttttagct gaagtg
//
LOCUS       DASWNE010000520         4255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_687673, whole genome shotgun sequence.
ACCESSION   DASWNE010000520 DASWNE010000000
VERSION     DASWNE010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687673"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..848)
                     /locus_tag="VGB68_10320"
     CDS             complement(54..848)
                     /locus_tag="VGB68_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049671.1"
                     /translation="MASASLPIRRSFGQTMRKGAWWAYPIVVFLALSAFVVYSTWAAF
                     QGEHYRFGNYLSPFYSPELIGSSEAIFGAKPGWIPDWLPFSPALLILWAPAGFRFTCY
                     YYRGAYYKAFWADPPACAVGEPRHSYRGERKFPLVLQNVHRYFLYLALIFIVFLAHDV
                     WEALWFTDAGGRTSFGVGVGTIVLALTVFFLAGYTFSCHSLRHLVGGKCDELSNKPIR
                     CATYNCVSALNRRHMVWAWLSLFFVGFSDIYVRLCSMGILYDWRIF"
     gene            complement(1285..2766)
                     /locus_tag="VGB68_10325"
     CDS             complement(1285..2766)
                     /locus_tag="VGB68_10325"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEY0049672.1"
                     /translation="MNQLTTYELTALARQALIEDGFMPDFPSPLTEAAQALDERRIIS
                     NAAARVKDLRALLWSSIDNAASRDLDQLEYVEKLPNGDIRLLVGIADVDEFVPKDSPI
                     DKFAAANTVSIYTGSRVFSMLPERLSTDLTSLLEGFDRLAVVTEIIISRNGDVETGNV
                     FRALVHNYAKLSYEKTGAWLEGNGEIPEPLVKISGMEAQIRLQQEAALRLRQFRKRNG
                     ALEFETVEAVPVIENDKITDLRTEKRNAARDIIENFMVTANVEMAEFLEKRRVVSLRR
                     VVRTPERWNRIVEIARSFNENLPENPDSFALAGFLARRRAADPAHYPDLSLSIIKLLG
                     ASEYVVQEAGADAGGHFGLAVSDYTHSTAPNRRYADLIVQRLVKATLAGEASPYTFEE
                     LSSIAAHCNKRESAARKSERQMRKTIAAGVMASRIGETFEAIVTGITAGGTFARITNP
                     PVDGRIVAGETGLQVGEKVRVRLIKTQPERGFIDFARASATEF"
     gene            complement(2825..3319)
                     /locus_tag="VGB68_10330"
     CDS             complement(2825..3319)
                     /locus_tag="VGB68_10330"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY0049673.1"
                     /translation="MKKSIIAVLLILGVFAGLAFSQTKKRPVRRGVKTTIKKTTTPKT
                     TMTTPKSTAVTTASGLTYIITQNGTGAQLKAGDTVIVHYTGLLTNGQKFDSSLDRAEP
                     FSFQLGARKVIKGWDEGLQKLRVGDRATFIIPPAIGYGSRGAGNVIPPDATLIFIVEV
                     VGAQ"
     gene            complement(3333..>4255)
                     /locus_tag="VGB68_10335"
     CDS             complement(3333..>4255)
                     /locus_tag="VGB68_10335"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY0049674.1"
                     /translation="DVEPSGESGLMGMTLHPDFVKNRFVYLAYAYRDAKGEQRVRVAR
                     YQETGASLADAKTIIENIPASRYHAGTRLRFGPDGKLYITTGDATNQSEAQKLGTIAG
                     KTLRLNDDGSVPADNPFVNQTGARPEIWTYGHRNAQGMDFQPGTDLMFQTEHGPSLID
                     GVSLFKRSGGDEVNIVEKGKNYGWAKISHQMRRAGMETPLAEYSPAIAPASGMFYRGN
                     LFPAFKGNFFIGALKGEMIIRLVLDGRRVISQDKLLEKQYGRIRDVAEAPDGSIYFST
                     SNRDGRGSAAKEDDRILRIVPLEDNKSETR"
BASE COUNT          966 a         1186 c         1104 g          999 t
ORIGIN      
        1 aaaaaataag gaattgtttt tactttatcc cttgtccttt cgcctttatc ctttcaaaag
       61 attctccagt catacaagat tcccatcgaa cagagacgca cgtaaatatc ggaaaagccg
      121 acgaaaaaca ggctcagcca agcccacacc atatgccgcc ggttcagcgc gctgacgcaa
      181 ttgtaagtcg cgcagcgaat cggcttgttt gaaagctcgt cgcatttgcc gccgaccaga
      241 tggcgcaggc tgtgacagct aaacgtatat ccggcgagaa agaaaaccgt cagcgccaga
      301 acaatcgtgc cgacgccgac gccgaaagaa gttctgccgc ccgcgtcggt aaaccacaag
      361 gcttcccaga cgtcgtgcgc gagaaagact ataaaaatca gcgccagata aagaaaataa
      421 cgatgcacgt tttgcagaac gagcggaaat tttcgctcgc cgcgatacga gtgacgcggc
      481 tcgccgacgg cgcacgccgg cgggtcagcc cagaaagcct tgtaatacgc gccgcgatag
      541 taataacagg tgaaacgaaa tccggctggc gcccacagga taagcaaagc gggcgaaaac
      601 ggcagccaat cgggaatcca gcccggtttc gcgccgaata tcgcttcgga agagccgatt
      661 agctcgggcg aataaaacgg cgagagatag ttgccgaaac gataatgctc gccctgaaaa
      721 gccgcccacg tcgaataaac gacgaaagcc gacaaggcta aaaaaaccac tatcggatac
      781 gcccaccacg cgcctttacg catagtttgc ccgaacgagc gtcttatcgg aagacttgcg
      841 gaagccatag tatttattaa tacctccagc gcaaattacg aaacgcgggc gtcggagccc
      901 cagggaaaat ataaggtgtg atatataaag acggctatct gctcgctttt ttaatcgctt
      961 ctgtttatcc ggcgcgccgc gcttaattta taaacttaaa ttgttctgat tcgataaccg
     1021 gttaattccg cccattttta cgttaatttt ctttgtaagt caagttaaga caataatata
     1081 ctcggcttac tccgtcgggc tccggcaaaa tgctgcgact cttctaattt aattcattcg
     1141 cggaaaaaat aaaatacttt ttattgcagc gggcggaaaa gaagccgggc aatcaggcag
     1201 cggagaaatt ggaaactgaa ttgtgtttgc tcaatctctc tgccggctcc gggattccct
     1261 gattccgcgg cttcttttcc gcctttaaaa ttccgtcgcg ctcgcccgcg cgaaatctat
     1321 aaaaccccgc tccggttggg ttttaatcag acggacgcgc actttttcgc cgacctgcaa
     1381 gccggtctcg cccgcgacga tgcgcccgtc gacgggcgga ttcgttattc gcgcaaacgt
     1441 gcctccggcg gttatgccgg tcacgatggc ttcgaacgtt tcgccgatgc gcgaagccat
     1501 cacgccggcg gcgatcgtct tgcgcatttg tcgttcggat ttgcgcgccg cgctttcgcg
     1561 cttgttgcaa tgcgcggcga ttgagcttaa ctcttcgaaa gtatacggcg aagcctcgcc
     1621 cgcgagcgtc gctttgacca gtcgctgcac gattaaatcg gcgtaacggc gattcggcgc
     1681 ggtcgaatgc gtgtagtcgg agacggctaa accgaaatga ccgcccgcat ctgcgcccgc
     1741 ttcctgcacg acgtattcgc tcgcgccgag cagcttgata atcgacaatg aaaggtccgg
     1801 ataatgcgcc gggtcagccg ccctgcgccg cgccagaaat ccggcgagcg caaacgaatc
     1861 gggattttcc ggcaaatttt cattgaagga acgggcaatc tcgacgatgc gattccatct
     1921 ttccggcgtt ctgacgacgc ggcgcagcga gaccactcgg cgcttttcca gaaattccgc
     1981 catctcgaca ttcgccgtca ccataaaatt ttcgataata tcgcgcgcgg cgttgcgttt
     2041 ttccgttctc aggtcggtga ttttatcgtt ttcgataacg ggaacggctt cgacggtttc
     2101 aaattccagc gcgccgtttc gttttctgaa ttggcgcagg cgcagggcgg cttcctgctg
     2161 gagtcgaatt tgcgcctcca ttcccgaaat tttgacgagc ggttcgggaa tctcgccgtt
     2221 tccctccagc cacgcacccg ttttttcata tgaaagcttg gcgtaattat gcaccagcgc
     2281 gcgaaaaaca ttgccggttt caacgtcgcc gtttcggctg ataattattt ccgtcacgac
     2341 cgcgagccgg tcaaatcctt cgagcagcga agtcaaatcg gtcgagagcc tttcgggcag
     2401 catcgaaaaa acgcggcttc cggtataaat cgaaaccgta ttggcggcgg cgaatttatc
     2461 aatcggcgaa tctttcggca cgaattcgtc aacatccgca attccgacca gcaggcgaat
     2521 gtcgccgttc ggcagttttt cgacgtattc caactggtcg aggtcgcgcg aggcggcgtt
     2581 atcaatcgaa gaccagagca gcgcgcgcaa atctttgacg cgagcggcgg catttgaaat
     2641 tatccggcgt tcgtccagcg cctgcgcggc ttcggtcagc ggcgacggaa aatccggcat
     2701 aaaaccgtct tcaatcaatg cctgccgcgc cagcgccgtc agctcgtaag ttgttagctg
     2761 gttcatttat ttaaaacgga aaacggaaaa tttgaaaacc tatattttcc atttcccatt
     2821 atttttactg cgcgccgacc acttccacga taaaaatcag cgtcgcgtcg ggcggaatga
     2881 cattgcccgc gccgcgcgaa ccgtagccga tggcgggcgg aataatgaaa gtggcgcggt
     2941 cgccgacgcg aagtttttgc agaccttcgt cccagccttt tattactttc ctcgcgccga
     3001 gctgaaacga aaacggctcg gcgcggtcga gcgagctgtc gaatttctga ccgttcgtca
     3061 aaagtccggt gtaatgaaca atgaccgtat cgcccgcttt taactgtgcg cccgtgccgt
     3121 tttgggtaat gatataggtt aatcccgatg cggtcgtgac cgccgtcgat tttggagtag
     3181 tcatagttgt tttcggtgtc gtcgtctttt taatcgttgt tttgacgccg cgtctgaccg
     3241 gtcgtttttt cgtctggctg aaagcgagac cggcgaaaac gccgagtatt aaaagaaccg
     3301 cgatgattga ttttttcata ttttcgtaag ctctatctcg tctccgattt attatcttcg
     3361 agcggcacga ttcgcaaaat gcggtcgtct tctttcgccg ccgagccgcg cccgtcgcgg
     3421 ttcgaagtcg aaaaataaat cgagccgtcc ggcgcttccg ccacgtcacg gatgcgcccg
     3481 tattgttttt ccagtaattt atcctgactg atgacgcggc gtccgtccag caccagacgg
     3541 ataatcattt cgcctttcaa cgcgccgata aaaaaattgc ctttgaatgc gggaaacaga
     3601 tttcctcgat aaaacatccc gctcgccgga gcaatcgccg gagaatattc cgcgagcggc
     3661 gtttccatcc cggcgcgccg catctggtgg ctgattttcg cccagccgta atttttcccc
     3721 ttttcaacga tattgacctc gtcgccgccc gaacgcttaa aaagcgaaac gccgtcaatc
     3781 aggctcggtc cgtgctcggt ttgaaacatc aagtcggtac cgggctgaaa atccatccct
     3841 tgcgcgttgc ggtgcccgta agtccagatt tccgggcgcg cgcccgtctg attaacaaaa
     3901 ggattatcgg cgggaacgct tccgtcgtcg ttcaaacgca gagtttttcc ggcgattgtg
     3961 ccgagtttct gcgcctcgct ctgattggtc gcgtcgcccg ttgtgatata gagcttgccg
     4021 tcgggtccga accgcagccg cgtgccggcg tgatagcgcg aggcgggaat attttcgata
     4081 atcgttttgg cgtccgccag gctcgcgccc gtttcctggt aacgcgcgac gcgcacgcgc
     4141 tgctcgcctt tagcgtcgcg ataagcgtag gcgagataga caaaacgatt tttgacgaaa
     4201 tccggatgca gcgtcattcc catcagaccg ctttcgcccg aaggctcgac atcgg
//
LOCUS       DASWNE010000521         2133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_687716, whole genome shotgun sequence.
ACCESSION   DASWNE010000521 DASWNE010000000
VERSION     DASWNE010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687716"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..1502
                     /locus_tag="VGB68_10340"
     CDS             210..1502
                     /locus_tag="VGB68_10340"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding domain-containing protein"
                     /protein_id="HEY0049675.1"
                     /translation="MTRRKFRNNPRSEPASQRSFQEYILNQPAPQTSRNELLRLIRGG
                     EDTYLELKIKLSNSERIAQEIVALANTAGGIVVFGVTDQLRVEGVDYAEDVQDELARI
                     CREEIYPPLVPLIDMIYFDSGRRVVALEVEGKRRPYRTRDGRFYLRIGTEKREATREE
                     LSMFLEEIRPLGYENIPVIGGSESDIDDALLWSFAKAFEDDANGRFTYNTAEFLKRDL
                     LLAVGNADEFIPTVAAVLLFGKNENVAELLPRSTVTAIRYSGDTTSAPIVEQTELKGN
                     LLTLFESAVSFIKRYSDLWNEKPKIKSDNGYSPILARANYSQNVILEAVVNALVHRDI
                     ALRDLQTRILVFDNFIEIINPRRTNGFVPPASRAIRYGITQRLNPQIAAIFSNPAYEA
                     NPVKSSLPQLLREAREFSGKRPEIYTSSDEFRLKIYGK"
     gene            complement(1629..>2133)
                     /locus_tag="VGB68_10345"
     CDS             complement(1629..>2133)
                     /locus_tag="VGB68_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049676.1"
                     /translation="VSVKAKRRAIILVASGIDTFSKTNYDKVRKVIQNSGIPIYVIST
                     GNLFFKMYEPYLPATDSISGSPGRLTFLQAKNVMNTFAKESGGQHFEMTFESELPSIL
                     NSINALLRNQYSLAYDAGEKLRDGKKYKLEVKVDVNGDGIYEEKEYIVQHRPFYNAPK
                     DEKNKKK"
BASE COUNT          524 a          546 c          545 g          518 t
ORIGIN      
        1 tgaaaatcgc cagttttcaa agctcaaagc tcgaaggtcg tccgtattca attccgcatt
       61 cgcaattttt aattcgaaat tcccctttcg ggcgcgtaat tgtctttttt ctcaataaat
      121 gattcaatta aggtgttgaa acttttattc cagcaatgcg cctgagccgg agcgcattcg
      181 ggcggataga gtgttccgtc ctgacaaaaa tgacacgcag aaaatttcgc aacaacccgc
      241 gcagcgagcc cgcttcgcaa cgctcctttc aagaatacat cttgaaccaa cctgcgccgc
      301 agacttcgcg gaacgagctt ttacgattga ttcgcggcgg cgaagacacg tatctcgaac
      361 tcaaaatcaa gctatcgaac tccgagcgca tcgcgcagga aatcgtcgcg ctggcgaaca
      421 cggcgggcgg cattgtggtt ttcggcgtga ccgaccagct gcgcgtcgaa ggcgtcgatt
      481 atgcggaaga cgtgcaggac gagctggcgc gcatctgccg cgaagagatt tacccgccgc
      541 tcgtgccgct gatcgatatg atttatttcg acagcgggcg gcgcgtcgtc gcgctcgaag
      601 tcgaaggcaa gcggcgaccg taccggacgc gcgacggcag attctacttg cgcatcggca
      661 cggaaaaacg cgaagcgacg cgcgaagagc tttcgatgtt tctcgaagag attcgcccgc
      721 tcggatacga aaatattccg gtcatcggcg gcagcgaaag cgacatcgac gacgcgcttt
      781 tatggagttt cgccaaagcg ttcgaggacg acgcgaacgg tcgttttaca tacaacaccg
      841 ccgagttttt aaaacgcgat ttgctgcttg ccgtcggtaa tgccgacgaa tttatcccga
      901 cggtcgccgc cgttttattg ttcggcaaaa acgaaaacgt cgccgagctt ttgccgcgct
      961 cgaccgtgac ggcgattcgt tattccggcg atacgacaag cgcgccgatt gtcgagcaaa
     1021 cggagctgaa aggaaatttg ctgacgcttt tcgaatcggc ggtaagcttt atcaaacgct
     1081 acagcgattt atggaacgaa aagccgaaaa ttaaatccga caacggttat tcgcctatct
     1141 tagcgcgcgc caattattcg caaaacgtga ttctggaagc ggtcgtcaac gctctggttc
     1201 accgcgatat tgctttgcgc gatttgcaaa cgcgaattct ggtcttcgac aattttatcg
     1261 aaatcatcaa cccgcggcgg acaaacggct ttgtgccgcc cgcctcgcgc gcgattcgct
     1321 acggcataac gcaacgcctg aacccgcaaa tcgcggcgat tttctcgaac ccggcttatg
     1381 aagcgaatcc ggtcaaaagc tcgctgccgc agcttctgcg cgaagcccgc gagttttccg
     1441 gcaaacgtcc cgaaatttat accagcagcg atgaattcag attgaagatt tacggaaaat
     1501 gataaaagtg gtaaacggaa aacggtaagt ggtaagttgc agttgcaaag tatcgaattt
     1561 ttatctaaaa cccgaaactt aaaacaacga acttaccact taccgcttac cggttaccac
     1621 tagaaaactt attttttctt gtttttctcg tctttcggcg cgttgtaaaa cgggcggtgc
     1681 tggacgatgt attctttttc ctcgtaaatg ccgtcgccgt tgacgtcgac cttgacttcg
     1741 agcttgtatt ttttgccgtc gcgcagtttt tcgcccgcgt cgtaagctaa actgtattga
     1801 tttcgcagga gagcgttaat cgagttgaga atcgagggca gctccgattc aaaagtcatt
     1861 tcaaaatgct gaccgccgga ttctttggca aaagtattca tcacgttttt cgcctgtaga
     1921 aaagtcaatc tgcccgggct gccgctgatc gaatcggtcg ccggaagata cggctcatac
     1981 attttgaaaa agagatttcc ggtgctgatg acgtaaatcg ggatgccgga gttctgaatg
     2041 actttgcgaa ccttgtcgta attggttttg ctgaaagtat cgatgccgga agcgaccagg
     2101 ataatggcgc ggcgctttgc cttgaccgaa acc
//
LOCUS       DASWNE010000522         4981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_614001, whole genome shotgun sequence.
ACCESSION   DASWNE010000522 DASWNE010000000
VERSION     DASWNE010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614001"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VGB68_10350"
     CDS             complement(<1..456)
                     /locus_tag="VGB68_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0049677.1"
                     /translation="MKFFFSIIAFALILSVALLAVPFSGTSAAPDSIYDLAILNARIV
                     DGTGNPWFRGAIGVKNGKIVKVGNINPSEAKETIDAQGKIVAPGFIDVHTHVEDIYTN
                     PNAENFVRMGVTSLVTGNCGGSATDIREFLGRMREKPLAVNLAALIGHNS"
     gene            713..1966
                     /locus_tag="VGB68_10355"
     CDS             713..1966
                     /locus_tag="VGB68_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY0049678.1"
                     /translation="MKQNQTDKRRLKKKERVFKLLLIACGFIFGLFVLEICLRVVDYS
                     YPEFYQPDELRGYALIPNMRGWYRKEGESFVRINSDGLRDDEHAPEKPADVFRIAVIG
                     DSYSEAPQVPVENAYWRVLKTRLEACGAFGSRRVETINFGVSGYSTTQELVTLREKVW
                     RYAPDMILLAMTTNNDMTDNLRHFKKTPIPYYVYQNGELVLDNSFRDDKSFRARNSTL
                     ARTGKWLHNHMRFVQAFHEIQVGLKYRYNAWRRGEVATQNQSSTQTNQTSAQTNIKPA
                     PPGETGIDNQVYRAPPDEFWKEAWRVTEGVLSLMKTEVENHRARFVVVTLSNGIQVNP
                     DAETREGFAKGVGADDLFYPDRRIKSWGDANGVEVINLAPDLLQYAERRQVLLHGFGE
                     NLGYGHWNAEGHRVAGETLAEKLCR"
     gene            2062..3885
                     /locus_tag="VGB68_10360"
     CDS             2062..3885
                     /locus_tag="VGB68_10360"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HEY0049679.1"
                     /translation="MYILGISAHYHDSAACLLKDGEIFAAAQEERFTRRKHDARFPSS
                     AIEFCLRRARIEMREVDFVVYYEKPLTKFERLLETYIDYAPSGLRSFIKAMPVWLKEK
                     LWTREEIRKSLSFEGQILFAEHHESHAASAFFPSPFESAAVLTIDGVGEWTTSSIGAG
                     AGNELNLLSELRFPHSLGLLYSAFTYYTGFKVNSGEYKVMGLAPYGEPRYVRKILDEL
                     VDLREDGSLRLNMKYFNFAQGLTMTNRRFDELFGGKPRAAEAELTRREMDLARSVQEV
                     TELAMLRMARHVHALTGEKNLCLAGGVALNCVGNGRILRETEFEKIWIQPAAGDAGGA
                     LGAALSVWHQYLGNERKVIENRDSMKGAYLGPDFSNGEIERDLNAAGAIYQRLPRTGL
                     IERVAELLAAGNVVGWFLGRMEFGPRALGARSILGDARSPQMQSQMNLKIKFRESFRP
                     FAPAVVREKVAEYFDLETDSPYMLLVAPVKESLRTEIGDEDKTLRGIEKLKTPRSTIP
                     AVTHVDYTARVQTVEPETNAMFYDLLQKFGELTGHPVLINTSFNVRGEPIVCSPAEAF
                     ACFARTEMDYLVVEDFLLDKKAQREELKIAAGDWQKEFPLD"
     gene            3939..4346
                     /locus_tag="VGB68_10365"
     CDS             3939..4346
                     /locus_tag="VGB68_10365"
                     /inference="COORDINATES: protein motif:HMM:NF039992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HEY0049680.1"
                     /translation="MQNGFAEPTREQARKTALVVAVVLILIAAFGWYRGRMTLAYVAG
                     AAALILIFNGLFVPPLARFFHVWWMRLAFALGYVNSRILRTIIYFLVFVPYGLVSRLF
                     RRDPLRRRAAKAESYWIPREETRQAREQFERMF"
     gene            4367..4525
                     /locus_tag="VGB68_10370"
     CDS             4367..4525
                     /locus_tag="VGB68_10370"
                     /inference="COORDINATES: protein motif:HMM:NF039953.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HEY0049681.1"
                     /translation="MKRFSLLREFWLFFRQNKKYWLLPMILILVAIGGLLIFAQSSAL
                     APFIYSLF"
     gene            complement(4534..>4981)
                     /locus_tag="VGB68_10375"
     CDS             complement(4534..>4981)
                     /locus_tag="VGB68_10375"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0049682.1"
                     /translation="SPPLRSRKHLEAILEALKDGTIDAIATDHAPHHADEKNLEFDRA
                     PFGITGLETAVGLAFEKLVHTGWLSLPRMVEMFSTNPAKIFNLKNRGTLKAGSIADVT
                     ILDPDLKWTYRVAETKSKSKNSPFDGWSFTGAAVATIVGGRIVYRR"
BASE COUNT         1165 a         1271 c         1407 g         1138 t
ORIGIN      
        1 cgaattatga ccgatgagcg cggcgagatt gacggcgagc ggcttttcgc gcattcgtcc
       61 gagaaattcc ctgatatcgg tcgccgagcc gccgcagttg ccggtcacca gggaagttac
      121 tcccatccgc acaaaattct ccgcgttcgg attagtgtag atgtcttcga cgtgcgtgtg
      181 aacgtcgata aatccgggcg cgacgatttt tccctgcgcg tcaatcgttt ctttcgcctc
      241 gctcggattg atatttccga ccttgacgat tttgccgttt ttgacgccga tcgcgccgcg
      301 aaaccacgga tttcccgtgc cgtcgacgat gcgcgcgttc agaattgcca gatcataaat
      361 ggaatcaggc gcagccgaag ttccggaaaa aggaacggca agcagagcga cgctcaaaat
      421 caaggcgaaa gcgattattg aaaaaaagaa tttcataaat tttatatacg ggagcggcag
      481 ataaattaaa acacgaacag tatattttat aacttcttta ccttcacggc ggcaaattga
      541 tttgacgaat aaattcagcg cgcgccgcgg cgaaagttcg gtgaatttaa ccagccgatt
      601 cgccgccgcg ttcgtcaacg agtcattatt ttttcgcaaa aataaaacac tgcttgtcag
      661 gcttttgcgg tttgcagtag aatgaggcgt tcaaacttcc cacgctcgaa aaatgaaaca
      721 aaaccagaca gacaaacgcc gcctgaaaaa aaaggagcgc gttttcaaat tgctgctcat
      781 cgcgtgcggt tttattttcg gcttgttcgt gctggaaatc tgcctgcgcg tggtcgatta
      841 ttcgtatccc gaattttatc agccggacga gctgcgcggc tacgcgctga taccgaatat
      901 gcgaggctgg tatcgaaagg aaggagaaag tttcgtccgg atcaactccg acggtctgcg
      961 cgacgacgaa cacgcgccgg aaaaaccggc ggatgttttt cgcatcgcgg tcatcggcga
     1021 ttcatattcc gaagcgccgc aggttccggt ggaaaacgct tactggcgag tgttgaagac
     1081 gcggctcgaa gcgtgcggcg cgttcggcag tcggcgcgtc gagacgataa atttcggcgt
     1141 ctccggttac agcacgacgc aggagctggt cacgctgcgc gaaaaagtct ggcgttacgc
     1201 gccggatatg attctgctcg cgatgacgac gaacaacgat atgacggaca atttgcggca
     1261 tttcaaaaaa acgccgattc cgtattacgt ttatcagaac ggcgagcttg tcctggacaa
     1321 tagttttcgc gacgataaat cttttcgcgc tcgcaattcg acgctcgccc gaaccggcaa
     1381 atggctgcac aatcatatgc gcttcgtgca ggcttttcat gaaattcagg tcgggctgaa
     1441 atatcgttac aacgcctggc ggcgcggcga agtggcgacg cagaatcaat cttcgacgca
     1501 aacgaatcaa acttcggcgc aaacgaatat aaaacccgcg ccgccgggcg agaccggcat
     1561 cgacaatcag gtttaccgcg cgccgcccga cgaattctgg aaggaagcgt ggcgcgtcac
     1621 cgaaggcgtt ttgtcgctta tgaaaacgga agtcgaaaat catcgggcgc ggttcgtcgt
     1681 cgtgacgttg agcaacggca tacaggtcaa cccggacgct gagacgcgcg aaggatttgc
     1741 caaaggcgtc ggcgcggacg atttatttta tcccgaccgg cgaatcaaaa gctggggcga
     1801 cgccaacggc gtcgaggtga taaacctcgc gccggatttg ctgcaatacg ccgagcgccg
     1861 acaggttctg ctgcacggct tcggcgaaaa cctcggctac gggcattgga acgcggaagg
     1921 tcatcgcgtc gcgggcgaaa cgctcgccga aaaactgtgt cgttaaatct ttgaccgcac
     1981 ggaagcggga acagaagaga acaacccgaa tttcttttcc tattctcaca ttctgaaatt
     2041 tcattacgct gaataaataa aatgtatatt ctgggcatct cggctcatta tcacgattcg
     2101 gcggcgtgtc tcctgaaaga cggggaaatc tttgccgccg cgcaggagga aagattcacg
     2161 cgccgcaagc acgacgcgcg gtttccgtcg tcggcgattg aattttgcct gcgccgcgct
     2221 cgcatagaga tgcgggaagt cgatttcgtc gtttattacg aaaagccgct gacgaaattt
     2281 gaacggctgc tcgaaaccta catcgactac gcgccgagcg ggctgcgctc gttcataaaa
     2341 gccatgccgg tgtggttgaa agaaaagctc tggacgcgcg aagagattag aaaaagctta
     2401 agtttcgaag ggcagatatt gtttgccgag catcacgaat cgcacgcggc gagcgcgttt
     2461 tttccgtcgc cctttgaaag cgcggcggtg ttgacgattg acggcgtggg cgaatggacg
     2521 acttcgagca tcggcgcggg tgcgggcaac gaattaaatt tattgagcga gctgcgtttt
     2581 cctcattcgc tcggtctgct ttattcggca ttcacttact acacgggttt taaagtcaat
     2641 tccggcgaat acaaagtgat gggactcgcg ccctacggcg agccgcgcta cgtccggaaa
     2701 attttggacg agcttgttga tttgcgcgaa gacggctcgc tccgtttgaa tatgaagtat
     2761 ttcaatttcg cgcaagggct gacgatgacg aaccgccgct ttgacgaatt gttcggcggc
     2821 aagccgcgag cagcggaagc cgaattgacc cggcgcgaga tggatttggc gcggtcggtg
     2881 caggaagtta cggaattagc gatgctcaga atggcgcggc acgttcacgc attgaccggc
     2941 gaaaaaaatc tgtgtctggc gggcggcgtg gctttgaact gcgtcggcaa cgggcgaata
     3001 ttacgcgaaa ccgaatttga aaaaatctgg attcaaccgg cggcgggcga tgcgggcggc
     3061 gcactcggcg cggcgctctc ggtgtggcat caatatctcg gcaacgaacg aaaggtgatt
     3121 gaaaatcgag attcgatgaa aggcgcgtat ctcggtcccg atttttcgaa cggggaaatc
     3181 gagcgtgatt tgaacgccgc cggagcgatt taccagcgcc tgccgcgaac cggattaatc
     3241 gaacgagtcg ccgagctttt agcggcggga aacgtcgtcg gctggtttct ggggcgtatg
     3301 gaattcggac cgcgagcgct cggagcgcgc tcgattctgg gcgacgctcg ctcgccgcaa
     3361 atgcagtcgc agatgaatct gaaaataaaa ttccgcgaat cttttcgccc gttcgcgccg
     3421 gcggtcgttc gggaaaaagt cgccgaatat ttcgacctgg aaaccgattc gccttatatg
     3481 ctcctggtcg cgcccgtcaa ggaaagtctg cgaacggaaa tcggcgacga agataaaacc
     3541 ttgcgcggca tcgaaaaatt aaaaacgccg cgctcgacga ttccggcggt gacgcacgtc
     3601 gattacacag cccgcgtcca aaccgtcgag ccggaaacaa acgcgatgtt ttacgatttg
     3661 ctgcaaaaat tcggcgagtt gaccggacac ccggttttaa ttaatacttc gttcaacgtg
     3721 cgcggcgagc cgattgtctg cagtcccgcc gaagcctttg cctgttttgc ccgaaccgaa
     3781 atggattatc tggtcgtcga agattttttg ctggataaaa aagcccaacg cgaagaatta
     3841 aaaatcgcgg cgggagattg gcagaaggaa ttcccgttag attaagtttc ggtaaaagaa
     3901 gcgctcgcac gacaacagtt agccctgact tgaaatatat gcaaaacggt tttgccgaac
     3961 cgacccgaga gcaagccaga aaaaccgcgc tcgtcgtcgc cgtcgtttta attttaatcg
     4021 ccgctttcgg ctggtatcgc gggcgaatga cgctcgctta cgtcgcgggc gcggctgctt
     4081 tgattttgat tttcaacggg ctgttcgtgc cgccgctcgc caggtttttt cacgtctggt
     4141 ggatgcgtct cgcctttgcg ctcggatatg taaacagccg gattctgcgg acgataattt
     4201 attttctcgt ctttgtgcca tacggtttgg tttcgcgtct gttcaggcgc gacccgctgc
     4261 gccgccgagc cgcgaaagcg gaaagctact ggattccgcg cgaggagacg cggcaggcga
     4321 gagagcagtt tgaaagaatg ttttgaataa ggataagatt agaaagatga aaagattttc
     4381 cttgctgcgc gagttctggc tgttcttccg gcagaataaa aaatattggc tgctgccgat
     4441 gattttgatt ttggtcgcca tcggcggatt gctgattttt gcccaatcgt ccgcgctcgc
     4501 gccgtttatt tactcgttgt tctagcctta tcttcagcgt cgataaacaa tgcgcccgcc
     4561 gacaatcgtg gcgaccgccg cgccggtaaa gctccagccg tcgaacggcg aatttttcga
     4621 tttcgatttc gtctccgcca cgcggtaagt ccattttaaa tccgggtcga gaatcgtcac
     4681 gtcggcaatg gagccggctt ttaaagtgcc gcgatttttt aaattaaaaa tctttgccgg
     4741 gttggtcgaa aacatttcga ccattctcgg aagactcagc cagcccgtgt gaaccagctt
     4801 ttcgaaagcc agcccgaccg ccgtctccaa gccggtgatg ccgaacggcg cgcggtcgaa
     4861 ttccagattt ttttcgtcgg cgtgatgcgg cgcgtggtcg gtcgcaatcg cgtcgatcgt
     4921 gccgtctttt aaggcttcga gaatcgcttc cagatgtttg cgcgagcgca gcggcggcga
     4981 c
//
LOCUS       DASWNE010000523         2291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_687886, whole genome shotgun sequence.
ACCESSION   DASWNE010000523 DASWNE010000000
VERSION     DASWNE010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687886"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1394)
                     /gene="uvrB"
                     /locus_tag="VGB68_10380"
     CDS             complement(<1..1394)
                     /gene="uvrB"
                     /locus_tag="VGB68_10380"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEY0049683.1"
                     /translation="MQFKLISENQPKGDQPEAIKQIVNGLDDGAKNQVLLGITGSGKT
                     FTIANVIQKVQRPTLVLAHNKTLAAQLYQEFKTFFPENAVEYFVSYYDYYQPEAYVPA
                     SDIYIEKEATINDEIDRLRLSATRALFERRDVIVVASVSCIYGLGDPDAYFGMIMFLE
                     PGQQLKREDLLKRLVELQYERSEIEFDRGSFRVRGDVVEVYPSYQEKAYRIEFWGDEV
                     DALYTIDPLLGEVLHKHESRLPIYPKSHYVMSKPTIKRAIQTIKDELVWWEEELIRQG
                     KLVEAQRLHQRTMYDLEMIKEMGFCRGIENYSRHLTGKPPGAPPPTLLDYLQEDALMV
                     IDESHQTIPQLGAMFKGDRSRKSTLVEYGFRLPSAMDNRPLNFEEFESRVGQTIYVSA
                     TPGPYELTKSGGEVIEQIIRPTGLLDPIVEVRPVKGQIDDLLGEVRARAERNERVLVT
                     TLTKKMSESLTEYFL"
     gene            complement(1474..>2291)
                     /locus_tag="VGB68_10385"
     CDS             complement(1474..>2291)
                     /locus_tag="VGB68_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049684.1"
                     /translation="PEGMPQPSTIFNESSGETGAPELNLPLPVFGFQKLEFSRPFNAL
                     PISNDEALLSAVEQTQEEYEEDEEIRDLAVRILQAFKTRNAVEALSQVALYDLSAPLR
                     SKAVSILADFDHESVFEAILLASADPTREVRAAAARAFTRLSFDRADAWTRIMESGEE
                     GKMRHAARAAIEGGYVERIFDRLVHRDPKNAYEAVALVALLIRAGETDLIFEKLKTSK
                     DAELQRAILHVIKITDDQSILNELYALLEQKYLPSDLRKTVDETIQAIGLVTA"
BASE COUNT          510 a          608 c          591 g          582 t
ORIGIN      
        1 agaaaatact cggtcaggct ttccgacatt tttttcgtca gagtcgtgac cagaactctc
       61 tcgtttcgtt cggcgcgcgc gcgtacttcg ccgagcaaat cgtcaatctg accttttacg
      121 gggcgaactt cgacgatcgg gtcgagaagt ccggtcgggc gaataatctg ctcgatgact
      181 tcgccgccgg attttgtcaa ttcataaggt ccgggcgtgg ctgaaacgta aatcgtctga
      241 ccaacgcgcg attcgaattc ttcgaaattc aacgggcgat tgtccatcgc cgacggcagg
      301 cgaaaaccgt attcgaccaa cgtcgatttt cttgagcggt cgcctttaaa catcgcgccg
      361 agctgcggaa tcgtctggtg cgattcgtca atcaccatca gtgcgtcttc ctgcaaataa
      421 tcaagcagag tcggcggcgg cgcgccgggc ggttttcccg tcaaatgccg cgaataattt
      481 tcgatgccgc gacaaaatcc catctcctta atcatttcca ggtcatacat cgtccgctga
      541 tgcaggcgtt gcgcttcgac gagttttccc tgtctgatta attcttcttc ccaccagacc
      601 agctcgtcct tgatggtttg aatcgcgcgt ttaatggtcg gcttcgacat cacgtagtgc
      661 gatttcggat aaatcggcag acgcgattcg tgtttatgca gaacttcgcc gagcagcggg
      721 tcaatcgtgt aaagcgcgtc gacttcgtcg ccccagaact cgatgcgata cgccttttcc
      781 tgatagctcg gataaacttc gacgacatcg ccgcgcactc gaaaagagcc tctgtcgaat
      841 tcgatttcgc tgcgctcgta ttgcagctcg accagtcttt ttaaaaggtc ttcgcgcttt
      901 agctgctgtc ccggttcgag aaacataatc atcccgaaat acgcgtccgg gtcgccgaga
      961 ccgtaaatgc aggagacgga agcgacgaca atcacgtcgc gccgttcaaa caaagcgcgc
     1021 gtcgcgctca gacgcagacg gtcgatttcg tcgttaatcg tcgcttcctt ttcgatgtaa
     1081 atgtccgagg cgggaacata agcttccggc tggtaataat cgtaatagga aacaaaatat
     1141 tcgacggcgt tttcgggaaa aaacgtcttg aattcctggt aaagctgcgc ggcgagcgtt
     1201 ttgttgtgcg ccagaacgag cgtcgggcgc tgaactttct gaatcacgtt ggcaatcgtg
     1261 aaagtttttc cgctgccggt aattccgagc agaacctggt ttttcgcgcc gtcgtctaaa
     1321 ccgttgacaa tttgcttgat agcttcgggc tggtcgccct tcggctgatt ttccgagatt
     1381 aatttgaact gcatagaaac aaaaacgcgc ttacaaagta tctttctatt ttacttcgta
     1441 aaccgcgtgt ttgtcactta tgaaataatg tttctacgct gtaacaagcc cgattgcctg
     1501 aatcgtctcg tcaaccgtct ttctcaaatc agatgggaga tatttctgct ccagcaaagc
     1561 gtaaagctcg tttagaatgc tctggtcgtc ggtgatttta atgacgtgca gaatcgcacg
     1621 ctgcaattcc gcgtctttgc ttgtctttaa tttttcgaaa attaaatcgg tctcgcccgc
     1681 tctaatcagc aaagcgacca gagccacggc ttcatacgcg tttttcgggt cgcgatgaac
     1741 gaggcggtca aaaattcttt cgacgtagcc gccctcgata gccgcgcgcg ccgcgtgacg
     1801 cattttgcct tcttcgcccg attccataat tctcgtccag gcgtcggcgc ggtcaaagct
     1861 gaggcgcgta aaagcgcgag cggcggcggc gcggacttcg cgggtcgggt cggcggaggc
     1921 gagcagaata gcttcgaaaa ccgattcgtg gtcgaaatcg gcgagaatcg aaacggcttt
     1981 tgagcgcagc ggcgcggaca aatcataaag agcgacctgc gacaaagcct cgactgcgtt
     2041 tcgcgttttg aaagcctgca gaatccgcac ggctaaatcg cggatttcct cgtcttcttc
     2101 gtattcttcc tgcgtctgct cgaccgcgct cagcaaagcc tcgtcattgg aaatcggcag
     2161 agcgttgaaa ggacgagaaa attcgagctt ttgaaatccg aaaaccggca gcggcagatt
     2221 taattccggc gcgcccgttt cgccggagct ttcgttgaaa attgtgctcg gctgcggcat
     2281 tccttcagga a
//
LOCUS       DASWNE010000524         3779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_548505, whole genome shotgun sequence.
ACCESSION   DASWNE010000524 DASWNE010000000
VERSION     DASWNE010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548505"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1714)
                     /locus_tag="VGB68_10390"
     CDS             complement(<1..1714)
                     /locus_tag="VGB68_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049685.1"
                     /translation="MEAEITSGSRLGRWMMQGVKEIEGPHEKEGKHPQHSWWQVMCLT
                     GVDYFSTLGYQPGIAFVAAGALSPIATLVLVLLTLFGALPIYNRVAAESPHGQGSISM
                     LEALLSRWKGKLFVLALLGFVATDFVITITLSAADASEHIIHNPFVEEHLHFLDHPIP
                     VTLFLIFILAAVFLKGFKEAIGIAVFLVIAYLLLNLIVVGVGLYQVFTHPEYLNNWKD
                     ALFSTKTLVGNFEIKGLLAVVALSLITFPKLALGLSGFETGVAVMPLVKNPNRIENTR
                     KLLTAAALIMSFFLICSSFMTSVLIPAAEFGEGGKAQGRALAYLAHTYLGDIFGTAYD
                     ISTILILWFAGASAMAGLLNIVPRYLPRYGMAPNWARATRPLVLVFGVICFAVTIIFK
                     ADVEAQGGAYATGVLVLMTSAAIAVTISAWAKKQAVKWAFMLIALVFAYTTITNVIER
                     PDGIKIASFFIFAIIAASFISRALRTTELRVEKIVFDDTARQFIDELAEGEIRIVTNR
                     RETGDIKEYRFKEHEKRMDNHIPSGDPVLFYEIEVGDASEFKGKLKVRGIDVGGYKIL
                     RTQAP"
     gene            complement(1833..3014)
                     /locus_tag="VGB68_10395"
     CDS             complement(1833..3014)
                     /locus_tag="VGB68_10395"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HEY0049686.1"
                     /translation="MFVDTVVTEIPNSSGGGRPSPESLLKMVQETERAKLRVYIGAAA
                     GVGKTFQMLEDANALRKQGIDVVIAVVETHGREETKEQIKDLEIVLPKKQEYRGAFFE
                     EMDLEAVIRRKPAVAIVDELAHTNVEGVKNRKRYEDVLELLENGISVITAVNIQHIES
                     LNDVVARTTNVKVRETVPDCFFKRADEIIDVDVSVDTLRTRLRQGKIYSVEKIEQSLN
                     NFFRKGNLAALRELALRQVAQFQSAQDHEYREREGLEQAVMPEKVMVCLASRGSAKKL
                     LRTGARIAGRLASDDWVAVYVETADEEAGRIKPEAFAALQENIRFAETLGAKVVKLKS
                     NKVADALLKFARENGITHVIFGQSARSRWEIIWKGSIINRFLREVKDAAVHVIPLEKE
                     D"
     gene            complement(3050..3622)
                     /gene="kdpC"
                     /locus_tag="VGB68_10400"
     CDS             complement(3050..3622)
                     /gene="kdpC"
                     /locus_tag="VGB68_10400"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010352921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HEY0049687.1"
                     /translation="MKDFITSALMILIFTVSLGGLYPLAVWGVGQVIFPHRANGSLIK
                     NAGGETVGSELIGQNFTGTGYFHSRPSAVGNGYDAGSSSGSNLGPTSEKLINRVKADT
                     EALRSENPKMPVPADLVTASASGLDPHISPAAAEFQIPRVAKERNISESDLQQIVARF
                     IEGRQFGIFGEPRVNVLLLNLELDKQLSEK"
BASE COUNT          961 a          991 c          929 g          898 t
ORIGIN      
        1 ccggcgcctg cgtgcggaga attttatagc ctccgacatc gattccgcga acttttaatt
       61 tacccttgaa ttccgaggcg tctccgacct cgatttcata gaaaagaacc ggatcgcccg
      121 acggaatatg attatccatc cgtttttcgt gctctttaaa gcggtattcc ttgatgtctc
      181 cggtctcgcg ccgatttgtg acgatcctaa tttcaccctc cgccaattca tcaataaact
      241 gccgcgccgt atcgtcgaag acgatctttt cgacgcggag ttcggtcgtg cgcagggcgc
      301 gggaaataaa cgaagccgcg ataatcgcaa aaataaaaaa tgatgcgatt ttgattccgt
      361 ccggtcgctc gatgacgttg gtaatcgtgg tgtaggcaaa aacgagggca atcagcataa
      421 aagcccattt taccgcttgt tttttcgccc aggcggaaat cgtgaccgca atcgccgccg
      481 aagtcatcag gaccagaaca ccggttgcgt aagcgccgcc ctgcgcctcg acgtccgctt
      541 tgaaaataat agtgacggcg aaacagatga ctccgaaaac cagaacgagc ggacgcgtcg
      601 cgcgcgccca gttcggcgcc atgccgtaac gcggcagata gcgcggcacg atattaagca
      661 gtccagccat cgccgacgcg cccgcaaacc ataaaattaa aattgtgctg atgtcataag
      721 ccgtgccgaa tatatcgcct aaatacgtgt gcgccagata ggctaaggcg cgcccctgag
      781 cttttccgcc ttcgccgaat tcggcggcgg gaatgaggac gctcgtcata aaactgctgc
      841 aaataagaaa aaagctcatg atcagcgccg ccgccgttaa aagctttctg gtattttcga
      901 ttcgattcgg atttttaacg agcggcatca cggcgactcc ggtttcgaag ccggaaagac
      961 cgagcgcgag tttcggaaag gtgatcagag aaagcgcaac gacggcgagc aatcctttta
     1021 tctcaaaatt tccgacgagt gttttagtcg aaaacaaagc atctttccag ttatttaaat
     1081 attcgggatg cgtaaatact tgatataaac cgactccgac gacgatcaaa ttcagcaaaa
     1141 gataagcgat aaccagaaaa accgcgatgc cgatggcttc cttaaaacct ttcaaaaaaa
     1201 cggcggcgag aataaaaatt aaaaacagtg tgaccgggat tgggtggtcg agaaaatgaa
     1261 gatgttcttc gacaaatgga ttgtgaatga tatgttcgct cgcgtcggcg gctgaaagcg
     1321 taatggtaat gacaaaatcg gtggcgacaa atcccagcag agcgagaacg aaaagctttc
     1381 ctttccaacg cgaaagcagc gcttcgagca tcgaaatcga accttgcccg tgcgggcttt
     1441 cggcggcgac gcggttgtag atcggcagcg cgccgaaaag cgtcagcaaa accaaaacga
     1501 gagtagcaat cggagacaaa gcgcccgcgg cgacaaaagc gatgccgggc tgataaccga
     1561 gcgtcgaaaa ataatcgacg ccggtcaggc acatcacctg ccaccacgaa tgctggggat
     1621 gttttccttc tttttcgtga ggaccttcga tttccttgac gccctgcatc atccagcgtc
     1681 cgagtctgct gccagaagtt atttccgctt ccataaaaac tttatccgta aattttcaaa
     1741 aaactcagga tacaaaaaaa gttataagga ttccgtaagg aatttataaa gaatgtataa
     1801 aagcgtattt tagagattaa aattatctta atctaatctt ctttttccag cgggatgacg
     1861 tgaacggcgg cgtcttttac ctcgcgcaga aaacggttga taatcgagcc tttccagata
     1921 atttcccagc gcgagcgcgc cgattgaccg aaaataacgt gagtgattcc gttttcgcgg
     1981 gcaaatttca gaagcgcgtc ggcgaccttg ttggatttca atttgacgac tttagcgccc
     2041 aaagtttcgg caaaacgaat attttcctgc aaagcggcga aggcttcagg cttgatgcgt
     2101 ccggcttcct cgtcggcggt ttcgacataa acggcgaccc agtcgtcgct ggcgagccgc
     2161 ccggcaatgc gcgcgccggt tcgaagaagt tttttggcgc tgccgcgcga agccagacaa
     2221 accatcactt tctccggcat aaccgcctgc tccaaacctt cgcgctcacg atattcatga
     2281 tcctgcgccg attgaaactg agcgacctgt ctgagcgcga gctcgcgcag agccgccaag
     2341 tttcctttgc gaaaaaagtt attcagcgat tgctcgattt tttctacaga atagattttg
     2401 ccttgtctca gccgcgttct gagcgtatct acggaaacgt cgacgtcgat gatttcgtcg
     2461 gcgcgtttga aaaagcagtc gggaaccgtt tcgcgcactt ttacatttgt cgttctggcg
     2521 acaacgtcat tcagcgattc gatatgctgg atattgaccg ccgtaattac ggaaatccca
     2581 ttttcgagaa gctccagcac gtcttcataa cgctttcggt tctttacgcc ttcgacgttt
     2641 gtatgagcga gctcatcaac tatggcgacg gcaggttttc gccggataac cgcctccaag
     2701 tccatctcct caaaaaatgc gccgcggtat tcctgttttt tcggcaacac gatttccaaa
     2761 tctttgattt gttccttcgt ttcttcgcgc ccgtgagtct cgaccaccgc gatgacaacg
     2821 tcgatgcctt gttttcgcaa agcgttggcg tcttcgagca tctgaaaagt tttgccgacg
     2881 ccggcagccg cgccgatata aacgcgcagc ttcgcccgct cggtctcctg caccattttg
     2941 agaagagatt cgggcgaagg gcgaccgccg ccgctgctgt taggaatttc ggttactacg
     3001 gtgtcaacaa acataacccg gattcgttta gaacattcgt ctttgccggt tatttttcag
     3061 aaagctgttt atcaagctct aaattcaaga gcagaacatt gacgcgcggc tccccgaaga
     3121 tgccgaactg cctgccttca ataaaacgcg cgacgatttg ctgcaaatcg ctttcactaa
     3181 tgtttctttc ctttgccacg cgcggaattt gaaattcagc ggcggcgggt gaaatatgcg
     3241 gatcgagtcc ggaagccgag gcggttacca ggtcagccgg aacgggcatc ttcgggtttt
     3301 ccgaccgcaa agcttcggtg tcggctttta cgcggtttat cagtttttcg ctcgtcggac
     3361 ccagattcga gccgctcgat gacccggcgt cataaccgtt tcccaccgcg ctcggacgcg
     3421 aatggaaata tccggtaccc gtaaaatttt gaccgataag ctctgaaccg acggtttcgc
     3481 cgccggcatt tttgatcagg gagccgtttg cccggtgcgg gaaaatcacc tgtccgacgc
     3541 cccaaaccgc cagcggataa agcccgccga gcgaaacggt gaaaatcaaa atcattaaag
     3601 ccgatgtaat gaagtctttc ataaatttta taaaccctaa accattccta aagcggtaat
     3661 tatcaaatcg atgatcttga tgccgacgaa cggcgcagca agaccgccga gtccgtaaat
     3721 cagcaagttt ctctgtaaca gccccgaagc cgacagagcg cggtatttga cgcctctga
//
LOCUS       DASWNE010000525         1785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_687950, whole genome shotgun sequence.
ACCESSION   DASWNE010000525 DASWNE010000000
VERSION     DASWNE010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687950"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VGB68_10405"
     CDS             complement(<1..443)
                     /locus_tag="VGB68_10405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0049688.1"
                     /translation="MKILIVDDEPLARELIREMLKAEKDCEFINEAKNGREAVELIRA
                     GAPDVVFLDIQMPDMDGFEVLEALSIKELLRIPAIIFVTAYDQYALQAFEYHALDYLL
                     KPFDRERFTTTLNRAREIVRHRDGGQERQQQQQILKMMEQMKAAP"
     gene            complement(464..1609)
                     /locus_tag="VGB68_10410"
     CDS             complement(464..1609)
                     /locus_tag="VGB68_10410"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY0049689.1"
                     /translation="MSAAERAKKRNKINWRHFGVGFLVWTIVGLSFALRTYFYNQRMG
                     MNISLAPLIISYLIDFYLWGLATPLIFLLCRRFPLERRHLLSHTLFHILSGAVFIFVI
                     IIVSVPLNWYLSFIDPALLPTMAALFYKTITSPIMLHESLLTYWATVIVAHAFEYYRE
                     ARERDAQAAQLAAQTARLSEQLVQAQLSALKMQLHPHFLFNTLNSIASLLHKDAETAH
                     RMIARLSDFLRMTLKNSETQSVSLDKELEFLDTYLEIEKIRFQERLEVEMRIEPEALA
                     AQVPNLILQPIVENAVRHGLANVASTGRLKISAGRVDNRVFIQIEDNGPGLNGRRKRK
                     TGGSGVGLANTRARLEQFYGADFSFEIAEKAESRGTIVSLEVPFKSR"
BASE COUNT          426 a          490 c          431 g          438 t
ORIGIN      
        1 tccggcgcgg ctttcatctg ctccatcatt ttcagaatct gctgctgctg ctgacgctct
       61 tgtccgccgt cgcggtggcg gacaatttct ctggcgcgat tcagcgtcgt ggtaaagcgc
      121 tcgcggtcga agggttttaa caaataatcg agcgcgtgat attcaaacgc ctgcaaagcg
      181 tattggtcgt aggcggtgac gaagataatc gcgggaatcc gcaaaagctc tttgatgctt
      241 aaagcttcca gcacttcgaa gccgtccata tccggcattt gaatgtcgag aaaaaccacg
      301 tccggcgcgc cggcgcgaat aagctcgacg gcttcgcgcc cgtttttcgc ttcattgata
      361 aattcacagt ctttttccgc tttcagcatc tcgcgtatca attcgcgcgc cagcggctcg
      421 tcatcgacta ttaatatttt cataacttct gccgacctgt aatttaccgg cttttaaacg
      481 gcacttcgag cgaaactatc gtgccgcgcg attccgcttt ctcggcgatt tcaaaactga
      541 aatccgcgcc gtaaaattgt tcgagccgcg cgcgggtgtt tgccagcccg acgccgctgc
      601 cgcctgtttt tcttttccgt ctgccgttta aacccggtcc gttgtcttcg atttgaatga
      661 aaacccgatt gtcgacgcgc ccggcgctga tttttagacg cccggtcgaa gcgacgttcg
      721 ccaatccgtg acgcaccgcg ttttcgacaa tcggctgcaa aatcaaattc ggcacctgcg
      781 ccgccagagc ttccggctca atccgcattt cgacttccag ccgctcctga aaacgaattt
      841 tttcgatttc cagataggtg tccagaaact ccagttcctt atcgagcgag acgctctgcg
      901 tttcggaatt tttcagcgtc atccgcagaa aatcgctcag ccgcgcgatc atccggtgcg
      961 ccgtttccgc gtccttgtgc aaaagcgacg cgatggaatt cagcgtgtta aaaagaaaat
     1021 gcgggtgaag ctgcattttc agcgcggaaa gctgcgcctg cacgagctgc tcggaaagac
     1081 gcgcggtctg cgccgccagc tgcgccgcct gcgcgtctct ctctctcgct tcgcggtaat
     1141 attcgaaagc gtgcgcgaca atcacggtcg cccaataagt cagcaggctt tcgtgcagca
     1201 ttatcgggct ggtaatcgtt ttgtagaaaa gcgccgccat cgtcggcaga agcgccgggt
     1261 cgataaaact cagataccag ttgagcggaa ccgatacgat tattatgacg aagataaaaa
     1321 cagcgccgct cagaatatga aaaagcgtgt ggctcaacag gtgtctgcgc tccagcggaa
     1381 accggcggca cagcagaaag atcagcggcg tagccaagcc ccacaggtaa aaatcaatca
     1441 gataggaaat aatcagcggc gccagcgaaa tattcattcc catccgctga ttgtaaaaat
     1501 acgtgcgcag ggcaaacgaa agcccgacaa tcgtccagac cagaaacccg acgccgaagt
     1561 gccgccaatt tattttattt ctttttttcg cccgctcagc cgccgacata ttttctcatt
     1621 agcgaacaaa gtttaaggct gataaaaccc gatttaaggt tcggtaatat ttcctttaag
     1681 attcataaaa ctctattccg ggttcgtaag actcgattta atgaacataa agcccggttt
     1741 agctgagcct taaatcaaat attcaggaac tataaagtcg ttttt
//
LOCUS       DASWNE010000526         6505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_329641, whole genome shotgun sequence.
ACCESSION   DASWNE010000526 DASWNE010000000
VERSION     DASWNE010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6505
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329641"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VGB68_10415"
     CDS             complement(<1..211)
                     /locus_tag="VGB68_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049690.1"
                     /translation="MEEVSKHEENKKNFVRKNFENQLRIHGFGFQYAVIEEIIRLYNA
                     EQCAFAPKFAEFPVEINNTNTHIDFI"
     gene            791..3940
                     /locus_tag="VGB68_10420"
     CDS             791..3940
                     /locus_tag="VGB68_10420"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0049691.1"
                     /translation="MKKIHILFFVILCCAVLTAAQEPFGSIEGTVSDPQGAIIQNAAV
                     TVRSIATNATRTAQTNENGQYRVLQLQPGFYEIRVTATSFKQTVLENVQVQVGQTAAV
                     DISLQVGGASETITVTDIAEAQIDRSDNTVSGVVNTIQIENLPLNGRNFLDLAQLQPG
                     TEKVDGGSFDPTKANFTGVSIGGQAGRSTQITVDGGSVVDNVVGTTVQNFSQEIVQEF
                     QIGLSNYSLSTGASASGSVNVVSRSGTNQFKGNAFGYFRDDKFAAFPALSRLDAANNI
                     PLAARSERIPFDRQQFGGTIGGPILKNKLFFFTSYERNNQDGSALRNPFSSPSFAGFT
                     ANPFDETLFTGKIDWVVSDRSSAFFRYSFNDNIQQGPFPLGSGIVPRQSASGIFQSND
                     QIVTNRSHNFTTGLSYALSTNISNNLVFNFSDFNNAINAATPGLPEIGLSFDPAFRTG
                     TNVLTPQTTLQKRYQLRDDLTWVKGNHTMNFGANYERTAIGGLFTFANPVRIRLFTPP
                     STETEADFLNAFVRDISMGVGSPNLPFNDTGETTNDRIQVYAGDNWKLNPRLTLNYGL
                     AYRWDSNLWNHDLPRPALIAGLFDGGTAPSKRDNNNFAPRVGFAWDVAGDGKTVIRGG
                     FGVYYDNTIDNLRLFERADLGPAGAQQFLIGVGLTSPVLAPLGGDGRFNDGQITLAQM
                     LALYPAVRADLESRISNCPLPTAIECTGNISGPIFATNFQVPYSLQYSIGVQRELPAN
                     LLLQVDYNYRKGLHEVLVYDANQADSIAGPRLSTFDHPVPYADSSGFSTYSGLLARVD
                     RRFRNGFQFTASYALSDFKAFGADALGLGAGLTNLNNFRDDFGPAGLDRRHRFVFSAI
                     YDLPLYKNDSSFLKRNILGNWTVSLISTAFSGTPQSIFLPDFVDLTGTGTFVSYLPGT
                     RGGSIGREIKSVEQINALIRNYNQTLAGGTDPQGTPLMALAELPLDTPVGDDSVISQD
                     FRLNKTISFNERYRLSFIGEVFNLFNVANLGTVGDFVLPHAGTPANEITTLRPTQRTT
                     SVFGTGGPRSFQFGVRFAF"
     gene            complement(4434..5384)
                     /locus_tag="VGB68_10425"
     CDS             complement(4434..5384)
                     /locus_tag="VGB68_10425"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEY0049692.1"
                     /translation="MNETFKRKDLLGIRTLSAGEILSLLKTAETFRDVSRREVKKVPA
                     LRGKTVINLFFEPSTRTRTSFELAAKRLSADAVNISTSTSSLTKGETILDTAMNLDAM
                     APHVIVVRHGSAGVPHQLAKASKASIVNAGDGANEHPTQALLDAMTIREHKGEIEGLK
                     VAILGDILHSRVARSNVHLLTRLGATVRVSGPNTLVPPDFLGLVENGLEICPNIETAL
                     ENADVVMVLRIQRERMDKAFFPTMREYSIYYGLTKDRLKLANKDAIVLHPGPMNRGIE
                     IASDVADGSSSLILNQVEYGLAVRMAVLYLLTGGSQNAAP"
     gene            complement(5430..5675)
                     /locus_tag="VGB68_10430"
     CDS             complement(5430..5675)
                     /locus_tag="VGB68_10430"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEY0049693.1"
                     /translation="MNEQEIYRYIEMTPFRGRKPVFRGTNITVEHIIDDLAKGLTVEN
                     ILKKHEALTPKHIQAVFVYVRAQMREFEILRLAYAAG"
     gene            complement(5679..6116)
                     /locus_tag="VGB68_10435"
     CDS             complement(5679..6116)
                     /locus_tag="VGB68_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049694.1"
                     /translation="MKKTLVFCLAFLFTGFGAAAAKAQTGNDIFGYYGIDGKVPAAFA
                     DIDVIHLAGSYGARQKPPVHGMIRPKKGGDFPLLKPALSGKNISFTTRAVGGISYIFT
                     GTFVKFPDNEQQEGVILRGVLQKFKGRTKIAEASVGFTYDAGD"
     gene            complement(6129..>6505)
                     /gene="pyrR"
                     /locus_tag="VGB68_10440"
     CDS             complement(6129..>6505)
                     /gene="pyrR"
                     /locus_tag="VGB68_10440"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEY0049695.1"
                     /translation="TRPIFGILDITLYRDDLSTVGANPIVNRTELDVDIEDKNIILVD
                     DVLYTGRTIRAALDQLMDFGRPRRVQLAVLIDRGREHRELPIQADFVGKVVPTKKSEI
                     IKVMLKQFDDEEAVGIFERPPE"
BASE COUNT         1529 a         1747 c         1631 g         1598 t
ORIGIN      
        1 gaatgaagtc tatgtgtgtg tttgtattgt taatttctac tggaaattct gcaaattttg
       61 gtgcaaaagc gcattgttcc gcattatata aacgaattat ttcttcaatt acagcatact
      121 gaaaaccaaa tccatgaatg cgaagttggt tttcaaaatt cttccttaca aaattctttt
      181 tgttttcttc atgtttcgat acttcttcca tcaaccatta acctctaaac aacttaacag
      241 tttgttccat cgacatatat taaatagaaa attattttcg gcgcgcggca actaaaaaac
      301 aatccggatt atcaaaataa gaagattaag cataaagacc gcttattgca aattttatat
      361 agaaaaatgt aatttatcgt aaatttatcc gaaattttaa ataaatttga ataggttcag
      421 gtcttgccgg tcaatctctg tcttttcaca ccatttaaat gtagtttcat cacaaatttt
      481 aacggatttt tgctttggct tttcgtttgc tagaaaataa atcgaacggc tgattatcag
      541 cgtttcgagg atttcccgca gttgccggaa attctttgca agaggttttc tacagaggta
      601 aatagaggta tgaaaagcac ttaaagccat caggcaagcg gcgcgctatc tggaaaatat
      661 aacgccgcga gtgatggctt taagcattta tgagacccag tcaaggctca ttgtcgcctg
      721 catttttcaa tgcggcaacc gtgcgattat accacttttc aaagcctttg tattttttaa
      781 ggagttgaaa atgaaaaaaa tacatatttt gttctttgtc attttatgtt gtgcggtttt
      841 gacggcggcg caggaacctt tcggttcaat cgagggcacc gtcagcgacc cgcagggagc
      901 aattattcaa aacgccgcgg tgacggtgcg cagcatcgcc accaacgcca cccgaaccgc
      961 acagaccaac gaaaacggtc aataccgcgt tctgcaattg cagcccggtt tttatgaaat
     1021 ccgcgtgacc gcgaccagtt tcaaacagac cgtgctggaa aacgtgcagg tgcaggtcgg
     1081 gcagaccgcc gccgtcgaca tcagcctgca ggtcggcggc gcgagcgaga cgataaccgt
     1141 gaccgacatt gccgaagcgc aaatcgaccg ctcggacaac acggtctcgg gcgtcgtaaa
     1201 tacgattcaa atcgagaacc tgccgctgaa cgggcgaaat tttctcgatt tggcgcaatt
     1261 gcagcccggc acggaaaagg ttgacggcgg gtcgtttgac ccgacgaaag ccaatttcac
     1321 gggcgtttcc atcggcgggc aagccggacg ctcgacgcag attacggtcg acggcggctc
     1381 ggtcgtcgat aacgtggtcg gcacgaccgt gcagaatttc tcgcaggaaa tcgtgcagga
     1441 atttcaaatc ggtctttcca attacagtct ttcgaccggc gcttcggcgt caggctcggt
     1501 caacgtcgtt tcgcggtcgg gaacgaatca gttcaaagga aacgctttcg gctatttccg
     1561 cgacgacaag tttgcggcat ttccggcgct cagtcgtctg gacgcggcaa acaatattcc
     1621 gctcgcggcg cgttccgaaa gaattccgtt cgaccgccag caattcggcg gcaccatcgg
     1681 cggtccgatt ctcaaaaaca aattgttttt ctttaccagc tacgagcgga ataatcagga
     1741 cggctcggct ttgcgaaatc cgttttcgtc gcctagtttt gccggtttta cggcgaaccc
     1801 gtttgacgaa accctgttta ccggaaaaat cgactgggtg gtcagcgacc gttcgagcgc
     1861 gtttttccgt tattcgttta acgacaatat tcagcaaggt ccgtttccgc tcggttcggg
     1921 catcgtgccg cgccagagcg cgagcggcat cttccagtcg aacgaccaaa tcgtgaccaa
     1981 tcgctcgcac aactttacga ccggcttgag ttacgcttta tcaaccaaca tcagcaacaa
     2041 tctggtcttt aacttcagcg attttaataa cgcaatcaat gcggcgacgc ccggattgcc
     2101 ggaaatcggt ctgtcgttcg accccgcttt ccgaacggga acgaacgtcc tgacgccgca
     2161 gacgacgctg caaaagcgct atcaactgcg cgacgatttg acctgggtca aaggcaatca
     2221 cacgatgaat ttcggcgcga attacgagcg caccgcaatc ggcggcttat ttacgttcgc
     2281 caatccggtt cgcattcgtc ttttcacgcc gccttcgacc gaaacggaag ccgattttct
     2341 aaacgctttc gtccgcgata tttcgatggg cgtcggcagc ccgaatctgc cgtttaacga
     2401 tacgggcgag acgacgaacg accgcattca ggtttacgcg ggcgacaatt ggaagctcaa
     2461 tccgcggctc actttgaatt acggtctggc gtatcgctgg gattcgaatc tgtggaatca
     2521 cgatttgccg cgcccggcgc ttatcgccgg tttgtttgac ggcggaaccg ctccgtcaaa
     2581 acgcgacaat aataatttcg cgccgcgcgt cggtttcgcg tgggacgtgg cgggcgacgg
     2641 gaaaaccgtc attcgcggcg gcttcggcgt ttactacgac aacacgattg acaacctgcg
     2701 ccttttcgag cgcgccgacc tcggaccggc gggcgcgcag cagtttctta tcggcgtcgg
     2761 gctgacgagt ccggtgctgg ctccgctcgg cggcgacggt cggtttaacg acggtcaaat
     2821 aacgctggcg cagatgctgg cgctgtatcc tgcggtcaga gccgacctcg aatcgcgcat
     2881 cagcaactgc ccgttgccga cggcgattga atgcaccgga aatatttccg gaccgatttt
     2941 cgccacgaat tttcaggttc cgtattcgct gcagtattct atcggcgtgc agagggaatt
     3001 gcccgccaat ctgcttttgc aggtcgatta caattaccgg aaaggcttgc acgaagttct
     3061 ggtttacgac gccaaccagg cggattcgat tgcgggaccg aggctttcga cttttgacca
     3121 tccggttccg tatgccgatt cctcgggctt ttcgacttat tccggtctgc tggcgcgcgt
     3181 tgaccgccgt ttccgcaacg gttttcaatt taccgccagc tacgcgctgt cggactttaa
     3241 agccttcggc gcggacgctc tgggattggg cgcgggtttg acgaatctga acaatttccg
     3301 cgatgatttc ggtcccgccg gcttggacag aagacatcgt ttcgtgttca gcgcgattta
     3361 cgatttgccg ctctacaaaa acgacagcag tttcctgaaa cgcaacattt taggcaattg
     3421 gacggtttcg ttaatctcga cggctttcag cggaacgccg cagagcatct ttctgcctga
     3481 tttcgtcgat ttgaccggaa ccggaacctt cgtcagttat ctgcccggca cgcggggcgg
     3541 ctcgatcggg cgcgaaatca aatcggtcga gcagattaac gccctgattc gcaactacaa
     3601 tcaaacctta gccggaggca cagacccgca gggaacgccg ctgatggctt tggcggagct
     3661 tccgctcgat acgccggtcg gcgatgattc ggtcatttcg caggactttc gcctgaacaa
     3721 gacgattagc ttcaacgaaa gatacaggct ttcctttatc ggcgaagtct tcaatctttt
     3781 caacgtcgcc aatctcggca ccgtcggcga tttcgtcctg ccgcacgccg ggacgcctgc
     3841 caatgaaatc acgacgctgc gcccgacaca gcgcacgacg agcgttttcg gcacgggcgg
     3901 accgcgctca ttccaattcg gcgtaagatt cgccttttag acgaatccga atttaacgca
     3961 gagaagcggg ctgaaaggct cgcttttttt cattccgggt aaaacgaacg gaaacgccgc
     4021 acacaacacg cgcaaagcga cacgcgccgg atgcaaattt gcaaatacca tctgcaagat
     4081 tgcaaacatc atctgcaaaa gtacaaatat catttgtaat cctggaaata ccgtctgtaa
     4141 tcttactgat accgtttgca agcttacaaa taccatccgt aatcttacaa ataccggacg
     4201 taatcttata aataccgcgt gtaatcttac aaataccgtt tgtagtctta caaataccgg
     4261 ctgtattatt acaaataccg cttgtaaatt cacaaatacc gcttgtaagt tcacaaatac
     4321 catctgtaac tttgcaaata tcgcctgtaa ctttccaact gatgtttaca tctctgcaaa
     4381 taacatttgc gatgccggtt ttgccggaaa acccggtatt tatgtatgaa ttgtcacgga
     4441 gcggcgtttt ggctgccgcc ggtgagcaga tacaggacag ccatccgcac ggcgagaccg
     4501 tattcgacct gattcaaaat cagcgagctc gagccgtcag ccacgtccga ggcgatttcg
     4561 atgccgcgat tcatcggtcc cgggtgcaga acgatcgcgt ctttgtttgc cagcttcaat
     4621 ctatctttcg tcaagccgta ataaatcgaa tactcgcgca tcgtcgggaa aaaagccttg
     4681 tccattcgct cgcgctggat gcgcagaacc atcacgacgt cggcgttttc caaagccgtt
     4741 tcgatgttcg ggcaaatctc caagccattt tcgaccaagc ctagaaaatc aggcggaacc
     4801 agcgtgttcg gtcccgaaac gcgcacggtc gcgccgagcc tggttaataa atgaacgttg
     4861 gagcgcgcga cgcggctgtg cagaatgtcg ccgaggatag ccactttcaa tccttcgatt
     4921 tcgcccttgt gctcgcgaat cgtcatcgca tcgagcaaag cctgcgtcgg atgctcgttc
     4981 gcgccgtcgc ccgcgttgac gattgacgcc ttgctcgctt tcgccagctg atgcggaacg
     5041 cccgccgaac catgcctgac gacgatgacg tgcggcgcca tcgcgtcgag attcatcgcc
     5101 gtgtcgagaa tcgtttcgcc tttcgtcagg ctcgaagtcg aggtcgagat attgacggcg
     5161 tcggcggaaa gacgcttggc ggcaagctcg aaactggtgc gcgtgcgggt cgaaggctca
     5221 aaaaaaagat tgatgacggt tttgccgcga agcgccggga cttttttgac ctcgcggcgc
     5281 gaaacgtcgc ggaaagtctc cgccgttttc aataaactca gaatctcgcc cgccgacaaa
     5341 gtacgaatgc cgagcaaatc tttgcgctta aaagtttcgt tcatcaaaat ctgcaaaaaa
     5401 agcaaggcgt ccaaccttgc tttcggaaat caaccggcgg cgtaagccag acgcaggatt
     5461 tcaaactccc gcatctgcgc ccgaacatat acaaagaccg cctgaatatg tttcggtgtc
     5521 aaagcttcgt gtttctttaa aatgttttcg accgttaatc ctttggcgag gtcgtcgata
     5581 atatgttcga ccgttatatt cgtgccgcga aaaacaggtt tccgaccgcg aaacggcgtc
     5641 atctcgatgt atcgatatat ttcctgttcg ttcatcgcct aatcgcccgc gtcataagta
     5701 aatccgacgc tcgcctcggc aattttcgtc ctgcctttga atttctgcaa aacgccgcgc
     5761 agaattacgc cttcctgctg ctcgttgtcg ggaaatttga cgaacgtgcc ggtaaatata
     5821 tagctgatgc cgccgaccgc ccgcgtggta aaggaaatgt ttttaccgct cagcgcgggt
     5881 ttcaataaag ggaaatctcc gccctttttc gggcgaatca tcccgtgaac cggcggtttc
     5941 tgccgcgcgc cgtagctgcc cgccagatga atcacgtcga tgtcggcaaa cgccgccgga
     6001 actttcccgt cgatgccgta gtaaccgaaa atatcgtttc cggtctgcgc ttttgccgcc
     6061 gccgcgccga aaccggtaaa cagaaacgcc aggcaaaaaa ctaaagtttt tttcatattg
     6121 atttttttct attccggcgg acgctcgaaa atcccgaccg cttcctcgtc gtcaaattgt
     6181 ttgagcatca ccttgataat ctcgctcttt ttcgtcggca cgaccttgcc gacgaaatcc
     6241 gcctgaatcg gcagctcgcg atgctcgcgc ccgcggtcga tcaaaacggc gagctggacg
     6301 cggcgcgggc gtccgaaatc catcaactgg tcgagcgcgg cgcgaatggt tctgcccgtg
     6361 taaagcacgt cgtcgacgag aatgatgttt ttgtcctcga tgtcgacgtc gagttccgtg
     6421 cggttgacaa tcgggtttgc gccgacggtc gaaaggtcgt cgcgataaag cgtgatatcc
     6481 agaattccga aaatcgggcg cgtgc
//
LOCUS       DASWNE010000527         6446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_301118, whole genome shotgun sequence.
ACCESSION   DASWNE010000527 DASWNE010000000
VERSION     DASWNE010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6446
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301118"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2272)
                     /locus_tag="VGB68_10445"
     CDS             complement(<1..2272)
                     /locus_tag="VGB68_10445"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0049696.1"
                     /translation="MNRKTMNAERGTRNLRFLVLHSSFIALLYLLPALPIIAQTLSGT
                     VADAQNAPIAGANVSLENQNKIVSETATDAEGKFSVSADNQTNPVLKITAKGFADYEK
                     IVRRTDFDAPLTIVLAARNLREEVTVSITQTETRLSETPASVVVLTRENLDATAAQTV
                     DDALRQIAGFALFRRSSSRTTNPTAQGANFRGLAGSGASRASVLLDGISINDAFGGWT
                     YWTRVPKSSIEQIEVLRGGASSLYGDAALSGAVNLQTKSIGGAPVLRFETSAGTQNTF
                     DANAFGAFGKNGWNFSASLDAYKTGGYIPVAETERGAADFYANSRYQNGFLTLEKRFR
                     KDSRQRGAGAEFLKGARIFLRGNLFSENRDNGTRLQTNRTYFRQAVFGADFPETAFGA
                     FQLRSVIEKQIYDQIFSGVSADRSSENLTRIQRVPSQAFGVNLLWSKAFGNHAAASAL
                     EFREVRGASDETIYANNRATSLIGAGGKERAFSVSAQDFWRASDKLNISFGARFDAWE
                     NKNAGSATRNLTTNQTAVVVFPNRNESAFSPRLAALYRVNGNFSLYAAFSKSFRAPTL
                     NELYRAFRVGNVLTLANENLKSERADNFEGGINFTRFSGRLNLRGNFFAAEVSRPVVS
                     ITLSSTPNLITRQRQNVGKTRARGLELDAEFLPAKDLRFSASYLLVASRVAEFPDNPD
                     LIGKFLPQIPPHQLNLQMFYRPPSRFSFGAQARISGAQFEDDLNNLRLRPFFSLDVFA
                     SYRAAKKVEIFTAIENI"
     gene            complement(2306..3418)
                     /gene="menC"
                     /locus_tag="VGB68_10450"
     CDS             complement(2306..3418)
                     /gene="menC"
                     /locus_tag="VGB68_10450"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HEY0049697.1"
                     /translation="MKIKSIELIEINLPLVHFFETSFGRTYERRIILTRVSDESGAEG
                     WGEVTCGETPSYNEEWTDAAWVTLEKILAPMTIGKEFESASQIWDAMKRVRGNRMAKA
                     AIETAGWDLEAKKRGVPLWKHLGGVNREIASGVSIGIQDSVAQLFEKIQIELDAGYQR
                     IKIKIAPHWDYEIIRQVREKFGDILLMGDANSAYSLADVDLFKRMDKFNLMMFEQPLA
                     HDDMLDHAKLQREIKTPVCLDESVKSPEDARKAIEIGAGKIVNVKLGRVGGHFQAKLV
                     EEACRTANIPVWCGGMLESGIGRAHNIAMSTLAGFTLPGDVSASKRYWHEDIIEPEVE
                     VTKNGTITAAEAPGIGFAVNRARIDKLTSRRKIVTI"
     gene            complement(3523..5025)
                     /locus_tag="VGB68_10455"
     CDS             complement(3523..5025)
                     /locus_tag="VGB68_10455"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0049698.1"
                     /translation="MKVFRFGGLSFALVAAFLCFVINASAQESASSASVSRTRAIVNS
                     NPQSTTSNSPNRASRNSNYYEPRVAFKLPDEVAVEEFVNYHRHRLPLPKSGQAVALDA
                     RWGNSEFSAARREAVLQIGFTTAAVNERTDLRPLNLSLVIDKSGSMAESDKMSRVKES
                     LRTMIGKLRPEDIISIVVFDTDAQVLFPASRIGDGYELKRAIDCIEPDGSTNLHAGLM
                     LGYREAQKNFRKDVTNRVILLTDGIANVGVVEPTKIAVESSEFNGLGIDLSTIGVGLD
                     LNNDLLRTLAERGRGLYHFVSDYQDIEKVFVNEVQALVSSVAKKVEVSVDYDANLQIE
                     KIYGYAPRVRANGFSINLDNMNNGLTQVVMVRFRARNATKNSLPVRVRLSYFDVQRGR
                     TIEEVQQVSLTPSRQDSCELLADGEVKKNYTIAELAQSLFDMTEAARRQNYREAENFV
                     NASTADAYRRYPNMEDKDIRFVLNIVEGYRRDLKAFNRQKSKSSDCGACR"
     gene            5218..5979
                     /locus_tag="VGB68_10460"
     CDS             5218..5979
                     /locus_tag="VGB68_10460"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0049699.1"
                     /translation="MRTEDFVYLYEVEDKLWWFAGMRAISAALLDEFCPPVVENILDI
                     GCGTGLNLKWLERYAEAEAIKGLDVEKTALDFCRERGENFLTQASATDLPFAGASFDL
                     VTSFDVLVQIPGEKADEKAIAEMHRVLKPKGVAFVRAAAYEWMRSGHDEALNTQRRYT
                     VGKLSQKLENAGFTVLRKTYANTLPFPLAVVRRLALKPIGLSDRGSDVKPFPPSLEWL
                     NRILTRALLSEAKFLKRPKAKFPFGLSAICVVRKT"
     gene            complement(6152..>6446)
                     /locus_tag="VGB68_10465"
     CDS             complement(6152..>6446)
                     /locus_tag="VGB68_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049700.1"
                     /translation="HHQIVTLAEIANKLINLTGRGAVVGVPFPPERQLTDVGNCNCSY
                     KKIEQVLGWRPQVDLDEGLKSTIEFYRQNRERYWSAPDENTVSKAYSSLRRVK"
BASE COUNT         1444 a         1752 c         1671 g         1579 t
ORIGIN      
        1 aaatgttttc gattgccgta aaaatctcta ctttttttgc cgcgcgatac gacgcgaaaa
       61 cgtcgaggct gaaaaacggt cgcagccgca aattgttcaa atcgtcttca aactgcgcgc
      121 cggaaatcct cgcctgcgcg ccgaaagaaa aacgcgacgg cgggcgataa aacatttgca
      181 gattcagctg atgaggcgga atctgcggca gaaattttcc gattaaatcg ggattatccg
      241 gaaattccgc gacgcgcgaa gcgaccagca gatagctcgc cgaaaagcgc aaatcttttg
      301 ccggcaaaaa ttccgcgtcc agctcaagcc cgcgcgcgcg cgttttgccg acgttttgcc
      361 gttgtctggt aatcaaattc ggcgtcgagc ttaaagttat gctcaccacg ggacgcgaga
      421 cttccgccgc gaaaaaattt ccgcgcaaat ttaaacgccc ggaaaagcgc gtgaaattta
      481 ttccgccttc gaaattgtcg gcgcgctcgg attttaaatt ctcgttcgcc agcgtcagca
      541 cgtttccgac ccggaaggcg cgataaagct cgttcaaagt cggcgcgcga aacgatttgg
      601 aaaacgccgc gtaaagcgaa aaattcccgt ttacgcgata aagcgcggca aggcgcgggc
      661 tgaaagcgct ttcattgcga ttcggaaaaa cgacgaccgc cgtttgattc gtcgttaaat
      721 ttctcgtcgc cgaaccggcg tttttgtttt cccacgcgtc gaatctcgcg ccgaaactga
      781 tgtttaattt atcgctcgcg cgccagaaat cctgcgccga aacggaaaag gcgcgttctt
      841 ttccgcccgc gccgattagc gaagtcgctc gattgttggc gtaaatcgtc tcgtcgctcg
      901 cgccgcgaac ttctcgaaac tcgagcgcgg acgcggcagc gtgattgccg aaagcctttg
      961 accacaaaag atttacgccg aaagcttgcg acggaacgcg ttgaatgcgg gttaaatttt
     1021 ccgagcttct atcggcggaa acgcccgaaa aaatctggtc gtaaatttgt ttttcgataa
     1081 cggagcgaag ctgaaacgcg ccgaaagcgg tttcgggaaa atccgcgccg aaaaccgctt
     1141 gccgaaaata cgtgcgattc gtttgcaggc gcgtcccgtt atcccgattt tccgaaaaca
     1201 aatttccgcg cagaaagatt ctcgcccctt ttaaaaactc cgcgcccgcg ccgcgctgcc
     1261 gggaatcttt cctgaatctt ttttccagcg tcagaaatcc gttttgataa cggctgttcg
     1321 cgtaaaaatc cgccgcgccg cgctccgttt ccgcaaccgg aatgtagccg ccggttttat
     1381 aggcgtcgag cgacgccgaa aaattccagc cgtttttgcc gaacgcgccg aaagcattcg
     1441 cgtcaaacgt gttttgcgtt ccggcggagg tttcaaaacg caaaacgggc gcgccgccga
     1501 ttgatttggt ctgtagattt acggctccgc tcagcgccgc gtcgccgtag agcgagctgg
     1561 cgccgccgcg caggacttcg atttgttcga ttgaagattt cggaacgcgc gtccaatacg
     1621 tccagccgcc gaaagcgtcg tttatcgaga ttccgtccag cagaaccgaa gcgcgggaag
     1681 cgccgcttcc tgccagcccg cgaaaattcg cgccctgcgc ggtcgggttc gtcgtccggc
     1741 tcgagctgcg gcgaaacaat gcgaaaccgg cgatttgtct taaagcgtcg tcgacggttt
     1801 gcgcggcggt cgcgtccaga ttttcgcgcg ttaagacgac gacgctcgcc ggagtttcgc
     1861 tcagacgagt ttcggtttgc gtgatggaaa cggtgacttc ttcgcgcaga ttgcgcgccg
     1921 ccaaaacgat tgtcaaaggc gcgtcgaaat ccgtccggcg aacgattttt tcgtaatcgg
     1981 caaaaccttt cgccgtgatt tttaaaaccg gattcgtttg attatctgcg gaaacggaga
     2041 atttcccttc ggcgtcggtc gcggtttcgg agacgatttt attttgattt tccagggaaa
     2101 catttgcgcc cgcgattggc gcgttttgcg cgtcggcgac ggttccggaa agcgtctgcg
     2161 caataatcgg cagagccgga agaagataca gaagcgcgat aaacgatgaa tgaagaacga
     2221 ggaatcgtaa attccgcgtc ccgcgctccg cgttcatcgt tttcctgttc atcccgttcg
     2281 agttcccctt gttcaagtcg aaaagctaaa ttgtaacaat ttttcgccgc gacgttaatt
     2341 tgtcgattcg cgcgcgattg accgcaaaac cgattcccgg cgcttccgca gcggtgattg
     2401 ttccgttttt cgtgacttcg acctccggtt cgataatgtc ttcgtgccag taacgcttcg
     2461 acgccgaaac atcgcccggc agagtgaaac cggcaagcgt ggacatagcg atattatgcg
     2521 ctctgccgat gcccgattcc agcattccgc cgcaccagac gggaatattt gccgttcgac
     2581 aagcttcttc gacgagcttt gcctgaaagt gcccgccgac gcgtccgagc ttgacgttga
     2641 cgatttttcc cgcgccgatt tcaatcgcct tgcgcgcgtc ttcgggcgat tttaccgact
     2701 cgtccagaca gacgggcgtt ttgatttcgc gctgaagctt cgcgtggtcg agcatatcgt
     2761 cgtgcgccag aggctgctcg aacatcatca aattaaattt gtccattcgt ttaaataaat
     2821 cgacgtcggc gagcgaataa gccgaattgg catcgcccat caacaaaata tcgccgaatt
     2881 tttcgcgcac ctgccggatg atttcgtaat cccaatgcgg cgcgattttt atttttatgc
     2941 gttgataacc ggcgtcgagc tcaatctgga ttttttcaaa taactgcgcg acggaatcct
     3001 gaatgccgat ggaaacgccg gacgcgattt cccggttgac gccgccgaga tgtttccaca
     3061 aaggcacgcc tcgttttttc gcttccaaat cccagccagc cgtctcgatc gcggctttcg
     3121 ccatccggtt gccgcgcacg cgcttcatcg cgtcccaaat ctgcgaagcc gactcgaact
     3181 cttttccaat cgtcatcggc gcgagaattt tttccagcgt cacccaggcg gcgtccgtcc
     3241 attcctcgtt atacgaaggc gtctcgccgc aggtcacttc gccccagcct tccgcgcccg
     3301 attcgtcgct cacgcgcgtt aaaattattc ttctctcgta agttctgcca aagctcgttt
     3361 caaaaaaatg gacgagcggc aggttgattt cgataagctc gattgatttg attttcatta
     3421 gcggaaaatg tgcgtgcggg cacaaataaa caggataagc aatttcgcct atcctgtcca
     3481 tcttgttccc ctctgcgctt gaatgttcag gcggctggaa aatcatctgc aagcgccgca
     3541 atcgctgctc ttgctttttt gccggttaaa ggctttcagg tcgcgccggt agccttcgac
     3601 gatatttaaa acgaatcgaa tatccttgtc ttccatattc ggatagcgcc ggtaagcgtc
     3661 ggctgtcgag gcattgacga aattttcggc ttcgcggtaa ttctgacggc gcgcggcttc
     3721 ggtcatatcg aaaagcgatt gggcgagctc ggcaatcgta taatttttct tcacctcgcc
     3781 gtcggctaaa agctcgcacg aatcctgtct gctcggagtt aagctgactt gctgcacttc
     3841 ttcgattgtc cgcccgcgct gcacgtcgaa atacgacagg cgaactcgca ccggcagaga
     3901 atttttcgtc gcattgcgcg cgcgaaaacg caccatcacg acctgcgtca gaccgttgtt
     3961 catattgtcc agattgattg aaaacccgtt tgcccgcacg cgcggcgcgt aaccgtagat
     4021 tttttcaatt tgcagattcg catcgtaatc gacgctgact tcgacttttt tcgccaccga
     4081 cgagacaagg gcttgcactt cgtttacgaa aaccttttcg atgtcctgat aatcggagac
     4141 aaaatgataa agcccgcgcc cgcgttcggc taaagtccgc aaaaggtcgt tgtttaaatc
     4201 caagccgacg ccgatcgtcg aaaggtcgat gcccagaccg ttgaattcgg aagattcgac
     4261 ggcgattttc gtcggctcga cgacgccgac attggcgatg ccgtcggtca ataaaataac
     4321 ccggttggtc acgtctttcc gaaaattttt ctgcgcttcg cggtagccga gcatcaatcc
     4381 ggcgtgaaga ttggtcgagc cgtccggctc gatgcagtca atcgcccgtt ttaattcata
     4441 gccgtcgccg atgcgacttg ccggaaacag aacctgcgcg tcggtgtcga aaacgacgat
     4501 tgaaatgatg tcttccggac gcagttttcc gatcatcgtc cgcagagatt ccttgacgcg
     4561 cgacatttta tcgctttccg ccatcgagcc gcttttatca atcaccagag acaaattcag
     4621 cgggcgtaaa tcggttcttt cgttgaccgc ggcggtcgta aaaccgattt gcagaacggc
     4681 ttcgcgccgc gccgcggaaa attcgctgtt tccccagcgg gcatcgagcg cgacggcttg
     4741 ccctgatttc ggcagcggca gacgatgccg gtgataattg acgaattctt cgaccgctac
     4801 ttcgtcgggc agcttaaaag cgacgcgcgg ctcgtaataa tttgaatttc tcgaagctct
     4861 attcggcgag tttgaagttg tcgactgcgg attggaattt acaatcgcgc gagttcggga
     4921 aaccgacgcc gaagaagcgc tctcctgcgc cgaagcattt ataacaaaac acagaaacgc
     4981 ggcgaccaaa gcgaacgaca atccgccaaa acgaaaaact ttcattactt tctccttgtg
     5041 tgataagaat tttccagact cggggagagc tttattaaaa gactttgcca aaccctttct
     5101 ctaaaggatt ctttgaaaac aaattaagtt gcttcggcgg cgagccgaaa catttccggc
     5161 tgccgcgatt cttttacttt tgcttcttga gcttttatcg aacacaatgt aaaaaatatg
     5221 cggacggaag attttgttta tctctacgag gtcgaggaca agctctggtg gtttgccgga
     5281 atgcgcgcga tttccgccgc gctgctggac gaattttgcc cgcctgtagt cgaaaacatt
     5341 ctggacatcg gctgcggaac gggtttgaat ctgaaatggc tcgagcgcta cgccgaagcc
     5401 gaagcaatca aagggctgga cgtcgaaaaa acggcgctgg atttttgccg cgagcgcggc
     5461 gagaactttt tgacgcaagc ctcggcgacc gatttgcctt ttgccggcgc gagttttgat
     5521 ttggtgacga gctttgacgt tctggtgcag attccgggcg aaaaggcgga cgaaaaagcg
     5581 attgccgaga tgcaccgcgt tctgaagccg aaaggcgtgg catttgtgcg cgcggcggct
     5641 tacgaatgga tgcgcagcgg acacgacgaa gctttaaata cgcagcgccg ctacaccgtc
     5701 ggcaaattgt cgcagaaact tgaaaacgcg ggctttacgg ttttgcgaaa gacttatgcg
     5761 aacactttgc cctttccgct cgccgtcgtc cggcgtctgg ctttaaagcc gatcggtttg
     5821 tcggacagag gctctgatgt aaaacctttt ccgccttctc tggaatggct gaaccgcatt
     5881 ctgacgcgag ctttgctttc cgaagcaaaa tttctgaaac gcccgaaagc gaaattccct
     5941 ttcggtctgt cggcgatttg cgtcgtcagg aaaacctgaa tctcgacata aagaacgtga
     6001 agaacacaaa gggagattca aagcaggctt cgcggcttga tgaagcaggc tgcacggctt
     6061 gataaaatct ttctctcatc cgcgtctatc tgcgtttatc tgtggttaat tttctttgtg
     6121 ttcttcgtgt cttcgcgcgg aaaatttctt atcacttaac gcgccgcagc gatgaataag
     6181 ctttggagac cgtgttttcg tccggcgcgc tccaataacg ctcgcgattt tgcctgtaaa
     6241 attcaatcgt gctttttaag ccttcgtcca aatcgacctg cggacgccag cccagaacct
     6301 gctcgatttt cttgtaactg caattgcagt tgccgacatc ggtcagctgg cgctccggcg
     6361 ggaaaggaac gccgacgacc gcgccgcgcc ccgtcaggtt aatcagctta ttggcaattt
     6421 ccgccagcgt gacgatttga tgatgc
//
LOCUS       DASWNE010000528         5200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_195146, whole genome shotgun sequence.
ACCESSION   DASWNE010000528 DASWNE010000000
VERSION     DASWNE010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5200
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195146"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4596)
                     /locus_tag="VGB68_10470"
     CDS             complement(<1..4596)
                     /locus_tag="VGB68_10470"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HEY0049701.1"
                     /translation="MNNFNFGEILSARKLTVIATCLMALGWVSLSFFPPSHLAAGAEN
                     TIGSGSIVLQTDEHFVRRLNLTANDLVYNSATQTIYASVPSAAGNIGNSLTQINPRTG
                     GLGASVFVGSEPNLLALADDNQTLYVTLEGSFSVRRFNLATQTPGLQFSLGREQNGGN
                     YTASDIAVAPGNPNLLAVARYRNYVSPPGGGVAIFDNGFQLPQTGPGHSAGANYLAFS
                     NSASTLYGGGIYEGLRTMTVAFNGVTESVTGTPNGVGRVKYVNGLIYSSGGQVINPVT
                     RTLLGTFANATSNAFAIDAAAGRAYYLVRDNFGSNNWTLKAYDVNTFIQIGSLAIPNI
                     SGDATSLVRWGGNGLAFRTTGGEVYFIQTTLIPSSEPIPTPTPTVSPTPFVTPTPHAA
                     FIRQLNLPANDIVYNQATQKIYASVPSAGGATTGNSVTVLNPTSGAIENSVFVGSEPT
                     KLALAGDAQTLYVNLDGAGSIRRFNTVDQTAGTQFLVGSDSWDGPFAISDLAVSPDNP
                     NVVAVARQARFTSPSQEGVAIFDNGVLRPQTGPGHSDGSFYLAFSNSETSLYGSGGSL
                     GLALMTITANGVAGNGSVPFSGGAIKFDNGLVYNDFGQVANPGTRTLVGTFTGLNSNS
                     FIPVVLPDSANGRVYFLVSGPSGGVQIRAYDINTFLLIGTVNVTGVSGSPTSLIRWGT
                     NGLAFRTTGGRVVILQTSLVSATEPVPTPTPSVSPTPTATPAYIPTFVRRVNLPNNDI
                     VFNNASGTIQASVPSTAGPVTGNTITEVNPQTGAIGTSTFVGSEPATLAQADDGRTLW
                     VRLNGAGNAIRRYDMQTRTPGLQFTPSSSFYPQDMAVMPGSPQTLAVAVRYSGVAIYD
                     DGVQRPGVSNGGVYAINRIEFSDSPATLYGYESESSGFRLVKFGVNASGVTGVSQGGG
                     LLYGYSTDMKFAGGRLYATSGRIIDPEAKTLIGTLAVSGSTLAVDTALGRVFYLSDNI
                     LSAYDINTFIKIGSITLPGFNGATPASLVRWGTNGLAFRTFNSSGESQIYIVQSALVS
                     SAGTVPTGLQLSAATYGVNENAQNLSVTVTRTGDISLATTVDYATTDGTATAGADYAA
                     ASGTLSFAPGETSKTVLVPLINDNFYEGSETFSLALGNPSGGNSLLVFPSSATVTIFD
                     NESRPFIFNNNATVMEPRVGTTTATFTVSLNNASTETVTVNYATANGTAVAGSDYAAT
                     GGTLIFNPLETTKTVTVLINADAANEGDETFFLNLSNATNASIAISQVTGTIRNFDAQ
                     LARRVPFDFDGDFKADISVFRPSDATWYASRSSDNSLIALNFGLSGDKLAPADFDGDG
                     LTDISVFRDGTWYRINSSDSQFVAVQFGQTGDLPVPADFDGDRKADITVFRPADGTWY
                     RVNSSNNQFVPQQFGTSEDKPVVGDYDGDGKSDLAVFRPSAASWYILRSSNGSFVGFQ
                     FGAAADIPIAADYDGDGKADVSVFRTGNWYRLNSSNGQFVAAQFGAASDIPVAADYDG
                     DGKTDVAVFRPADGTWYINGTVNGFTGVQFGAAGDKPV"
     gene            complement(4918..>5200)
                     /locus_tag="VGB68_10475"
     CDS             complement(4918..>5200)
                     /locus_tag="VGB68_10475"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY0049702.1"
                     /translation="FAPTAATASVSGRILTSDGRGIRNVSITMTDANGAMRMAFSGSF
                     GYFTFEDVRVGEVYVLSVRAKQFRFGQSSLAISVQEDLSDISFTADPLR"
BASE COUNT         1132 a         1452 c         1515 g         1101 t
ORIGIN      
        1 aacgggtttg tcgcccgccg ctccgaattg aacgccggta aaaccgttca ccgtcccgtt
       61 tatgtaccag gttccgtccg ccggacggaa cacggcgaca tctgttttac cgtcgccgtc
      121 gtaatcggct gcgaccggaa tatcgctggc agcgccgaat tgcgcggcga cgaattgtcc
      181 gttcgagctg tttagacggt accagttacc ggtgcgaaag accgaaacgt ccgctttacc
      241 gtcgccgtcg taatcggcgg cgataggaat gtccgccgcc gctccgaact ggaatccgac
      301 gaacgagccg tttgagctcc gcagaatgta ccacgaagcg gcagacggac ggaagacggc
      361 taaatcgctt ttcccgtcgc cgtcgtaatc gccgaccacc ggtttgtctt cgctcgttcc
      421 gaactgttgc ggaacgaact ggttgttcga gctgttcacg cggtaccagg ttccgtccgc
      481 cggacggaac acggtgatgt cggcttttcg gtcgccgtcg aaatcagcgg gaaccggcaa
      541 atcgcccgtc tgcccgaact gaacggcgac gaattgactg tccgagctgt ttattcgata
      601 ccatgtgccg tcgcgaaaga ccgagatgtc ggtcaatccg tcgccgtcga aatcggctgg
      661 cgcgagctta tcgccgctca gtccgaagtt taaggcgatg agcgagttgt cggaactgcg
      721 cgacgcgtac caggtcgcgt cggacgggcg aaatacggaa atgtcggctt tgaaatctcc
      781 gtcgaaatcg aaaggaacgc ggcgcgccag ttgcgcgtcg aagtttcgaa tcgttcccgt
      841 cacctgagaa atggcgattg aagcgttggt cgcgttgctc aaattcaaaa agaaagtctc
      901 gtcgccttcg ttcgccgcgt cggcattaat cagaaccgta acggtctttg tcgtctcgag
      961 cgggttaaaa ataagcgtgc cgccggtcgc cgcgtaatcg ctgcccgcga cagccgtccc
     1021 gttggcggtc gcgtaattaa ccgtcacggt ttcagtcgag gcattgttca ggctgaccgt
     1081 aaatgtagcg gtcgtcgtgc cgacgcgcgg ctccatcacg gtcgcgttat tgttgaaaat
     1141 aaagggacgc gattcgttgt cgaaaatcgt gacggtcgcc gacgacggga aaaccagcag
     1201 agagtttccg cccgacggat tgccgagcgc caggctgaac gtttcgctgc cctcgtaaaa
     1261 attatcatta atcagcggca cgagaacggt cttgctcgtt tcgccgggcg caaagctgag
     1321 cgtgccggaa gcggctgcat aatcggcgcc tgccgtcgcg gttccgtcgg tggtggcgta
     1381 atcgacggtc gtcgccaaag aaatgtcgcc cgtccgcgtc acggtgacgc tcaggttttg
     1441 agcgttttcg ttcacgccgt aggtcgcggc gctcagctgt aagccggtcg gaacagtgcc
     1501 cgccgaagaa accagcgcgc tttgaacgat gtaaatctgg ctttctccgg acgaattgaa
     1561 tgttctgaaa gccaagccgt tcgttcccca gcggacgagc gaggcgggcg tcgcgccgtt
     1621 gaaaccggga agcgtgatgg aaccgatttt gataaaggta ttgatgtcgt aagcagacaa
     1681 aatattatcg gacaggtaaa acacgcgacc gagagccgta tcgaccgcca gcgtcgagcc
     1741 gctgaccgcc agagttccga tcagcgtttt cgcttccgga tcgattattc tgccggacgt
     1801 ggcgtaaagc cgcccgcccg caaatttcat atccgtgctg tagccgtaca gtaagccgcc
     1861 gccctgcgaa actccggtga cgcccgaagc gtttacccca aatttaacca gcctgaaccc
     1921 gctcgactcg ctttcgtagc cgtaaagcgt cgcgggcgaa tccgaaaatt cgatgcggtt
     1981 gatggcgtaa actccgccgt tgctcacgcc gggacgctgc acgccgtcgt cgtaaattgc
     2041 cacgccgctg tatctgaccg ccaccgcgag agtttgcggg ctgcccggca taaccgccat
     2101 atcctgcggg taaaaagaac tgctcggcgt aaactgcaaa ccgggcgtgc gggtttgcat
     2161 atcgtaacgc cggatggcgt tgcccgcgcc gtttagcctg acccacagcg tgcgcccgtc
     2221 atcggcttgc gccagcgtcg ccggttcgct gccgacgaag gtcgaagttc cgatagcgcc
     2281 ggtttgcgga ttgacttcgg tgatggtgtt tcccgtcacg ggacccgccg tgctgggcac
     2341 gctcgcctga atcgttccgc tcgcgttgtt gaaaacgatg tcgttgttcg gaagattgac
     2401 gcgccggacg aacgtcggaa tataagccgg cgttgccgtc ggcgtcggcg aaacgctcgg
     2461 cgtcggcgtc ggaaccggct cggtcgccga aacgagcgag gtttgcagaa tgacaacccg
     2521 cccgccggtt gttctaaacg ccaaaccgtt tgttccccag cgaatcaggc tcgtcggcga
     2581 gccggaaacg cccgtcacgt ttaccgttcc gatcagcaga aaagtattta tgtcgtaagc
     2641 gcgaatctgg acgccgccgc tcggaccgct gaccagaaaa tatacacgcc cgttcgccga
     2701 atcgggcagc acgaccggaa tgaaagaatt actgttgaga ccggtaaatg tcccgaccag
     2761 tgttctcgtg cccggattag cgacctgtcc gaagtcgttg taaaccaagc cgttatcgaa
     2821 ctttatcgct ccgccggaaa acggcacgga gccgttgccc gcaacaccgt tggcagtgat
     2881 tgtcataagc gccaagccga gcgagccgcc ggagccgtaa agagaggttt cggaattcga
     2941 gaaggcgaga taaaaactgc cgtcgctgtg tccgggaccg gtttgcgggc gcagcacgcc
     3001 gttgtcgaaa atcgccacgc cttcctgcga gggacttgta aaacgcgcct gcctggcgac
     3061 ggcgacgaca ttcggattat ccggtgagac cgctaaatcg ctgatagcga aaggaccgtc
     3121 ccagctgtcg ctgccgacca gaaattgtgt tccggcggtt tggtcgaccg tattgaagcg
     3181 cctgatggag ccggctccgt ccagattgac gtacagcgtc tgcgcgtcgc cggcgagcgc
     3241 cagttttgtc ggctcgctgc cgacaaagac ggaattttct atcgcgccgc tggtcgggtt
     3301 aagaaccgtc acgctgttgc cggtcgtcgc gccgcccgcg ctcgggacgc tcgcataaat
     3361 cttttgcgtc gcctgattgt aaacaatgtc gtttgccggc aaattaagct ggcggatgaa
     3421 agccgcgtgc ggcgtcggcg tgacaaacgg ggtcggcgaa acggtcggcg tcggcgtcgg
     3481 gatcggctca gaggatggaa tcaaagtggt ctgaataaaa taaacttcgc cgcccgtggt
     3541 tctgaaagcc aacccgttgc cgccccaacg gacgagcgaa gtcgcatcgc cgctgatgtt
     3601 cggaatcgca agcgagccga tctggataaa ggtgttgacg tcgtaagctt tcaacgtcca
     3661 attatttgaa ccgaaattgt cacgcaccag gtagtaagcg cgaccggcgg cggcgtcaat
     3721 cgcgaaagcg ttactcgtag cgttggcgaa agttcccagc agagttcgcg tgaccggatt
     3781 tataacctgt ccgcccgaac tgtagataag cccgttgacg tatttgacgc gcccgacgcc
     3841 gttcggcgtt cccgtgacgg attccgtcac gccgttaaag gcgacggtca tcgtgcgaag
     3901 cccctcatat atgccgccgc cgtaaagggt cgaagcggaa tttgaaaaag ccagataatt
     3961 ggctccggcg ctgtgtccgg gaccggtctg cgggagctga aagccattgt cgaaaatcgc
     4021 tacgccgccg cccggcgggc tcacgtaatt gcggtatctg gcgaccgcga gcagattcgg
     4081 atttccgggg gcgacggcaa tgtcgctggc ggtataattg ccgccgtttt gctcgcgccc
     4141 gagcgaaaac tgcaaaccgg gcgtttgagt cgccaggtta aagcggcgaa cggaaaaaga
     4201 gccttcgagc gttacatata acgtttgatt gtcgtcggcg agggcgagaa ggttcggctc
     4261 gctgccgaca aaaaccgacg ctccgagccc gcccgtccgc ggattgattt gcgtcaggct
     4321 gttgccgata ttcccggctg cgctcgggac gctcgcatag attgtttgcg tggcggaatt
     4381 gtaaacgaga tcgttcgcgg tcagatttag tcgcctcacg aaatgctcgt ccgtctgcaa
     4441 aacaatcgaa ccggaaccaa tcgtattttc agcgccggcg gctaaatgac tggggggaaa
     4501 aaaggaaagc gaaacccagc cgagcgccat taaacaggtg gcaatgacgg ttagtttccg
     4561 ggcggataaa atttccccga aattaaaatt attcattaag aaactcctaa atttttgcta
     4621 aaataatttg acaatcatac tgcggagaga gagacctaaa ggttacttgc cggcaaataa
     4681 atgagttgtg attgattatc ctgttttttg attttttgat gagggcacag gcactctgac
     4741 actagcaaaa ttttccaaaa aaatcaaata ttaaatttca aagacgatac gaaaattacg
     4801 gcttccgaaa cgaatttacg tctgcccgtt ttgccgaaaa agtgttttat tccaatattt
     4861 cccggacgac gaatgattcg tttgacggat tttattattc gaatcgcttt cacgggctta
     4921 ccggagcggg tcggcggtaa aactgatgtc ggacaaatct tcctggacgg aaatcgccag
     4981 cgagctttga ccgaatctga attgcttggc gcggacgctc aggacataaa cttctccgac
     5041 ccgcacgtct tcgaacgtaa aatagccgaa actgccgctg aacgccattc gcatcgctcc
     5101 gttcgcgtcg gtcatcgtta tcgaaacgtt acggatgccg cgcccgtccg aggtcagaat
     5161 tctgccgctc acgcttgccg tcgcggcggt cggcgcaaac
//
LOCUS       DASWNE010000529         3396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_614294, whole genome shotgun sequence.
ACCESSION   DASWNE010000529 DASWNE010000000
VERSION     DASWNE010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614294"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VGB68_10480"
     CDS             complement(<1..367)
                     /locus_tag="VGB68_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049703.1"
                     /translation="MKLTKTSVCVLLVTFLVGYASVSPPATKQIAREMPGDVSTVAPA
                     AAPPFYVDGEPETVKENDNRDFDEKYPFKIKLLETGEGFHGDEVEAKSGETWLGLFDE
                     NGRHVLRRTKIKISRVHDPV"
     gene            complement(371..715)
                     /locus_tag="VGB68_10485"
     CDS             complement(371..715)
                     /locus_tag="VGB68_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049704.1"
                     /translation="MNQPTLFESGTQSVELESLTLYALGEFQSRGKVLAQRELALDRL
                     RGAFKRASEHFQIAELKDEKIAETLEKLGAKVVKVPNFVAKHPFRVTVQQALAERAFE
                     FYRETISQKAEA"
     gene            850..2124
                     /gene="coaBC"
                     /locus_tag="VGB68_10490"
     CDS             850..2124
                     /gene="coaBC"
                     /locus_tag="VGB68_10490"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEY0049705.1"
                     /translation="MSDSNPKFRVGLGVAGGIAAYKAIEVLRRLQKSGCEVQAAMTKH
                     ATEFIQPLTFRALTENYVLVDDYAPENPDPIAHINFSQNIDLLLIVPATANLLAKFAN
                     GIADDFLSSTFLASSAPVLVAPAMNTTMWFHPATQRNIERLKKDGVLFVEPVSGELAC
                     KTVGTGKLEDVENIVAQALRLLKSEIGNRKSESESVSPDAAPGIQDLKGERLLITTGG
                     TREEIDPVRFISNHSSGRMGFALAEAAQKRGAQVTVVAASTSIAPPENVKITSAISAA
                     AMHRAVMNELNDATVFVGAAAVADYRPANRAENKIKKTGDRLVIELEKTTDILAEVSK
                     KRPSGLIVVGFAAETNDVEAHARSKMHRKHLDLIVANDVSNDKTGFGSENNQGLILSR
                     DAKNPTEISLMSKREMADKILDEVVKFRKRNG"
     gene            2117..3145
                     /locus_tag="VGB68_10495"
     CDS             2117..3145
                     /locus_tag="VGB68_10495"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY0049706.1"
                     /translation="MAENLHNEYGALVAALKEQILYLQELGVENFNVDLPELDPKFQN
                     AGAFRRPQTEDRKPNLEKFVPDAPVAAAPPIAKTEQPIAKSEPNTVRQNLLEKSKLSR
                     LPSLPKRGANVSNFQAESQISEPAKSDAMPKQKTTAEENLNQSLFGDIKQTLPDAGET
                     LEDIRLDIGNCTRCPLWEGRTKIVHSEGNPQADLVFVGEAPGANEDAEGRPFVGKAGQ
                     LLNKIIEAIGMKREDVFIGNINRCRPPGNRTPTLPEAHTCRPFLLREIAVIRPKVIVV
                     LGNTATQNLLDTKIGITKLRGEFQDYYGVKVMPTFHPAYLLRDPSKKRETWEDMKRVR
                     EEMNKLKG"
     gene            3208..>3396
                     /locus_tag="VGB68_10500"
     CDS             3208..>3396
                     /locus_tag="VGB68_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049707.1"
                     /translation="MKTKIIANNDQAVIVLPRESMQILGVEVGDEVEFSKEADAVVLR
                     SSSEAERKRKFEKAKREIF"
BASE COUNT          891 a          885 c          878 g          742 t
ORIGIN      
        1 caaccgggtc gtgaacgcgg ctaattttta ttttcgtccg gcgcaggacg tgtctgccgt
       61 tctcatcgaa aagaccgagc cacgtttcgc cggatttcgc ttccacttcg tcgccgtgaa
      121 agccctcgcc ggtttccaga agctttattt tgaacggata tttttcatca aaatcccggt
      181 tatcgttttc tttgacggtt tccggctcgc catcgacata aaacggcggc gcggcggctg
      241 gcgcgacggt cgaaacatcg cccggcattt cccttgcgat ttgtttcgtc gcgggcggcg
      301 aaacggatgc atagccgacc agaaaagtga ccaataaaac acaaacggaa gtttttgtga
      361 gtttcatagc ttatgcctca gctttttgcg aaatcgtttc gcggtaaaat tcaaaagccc
      421 gctccgccag agcctgttga accgtcacgc gaaacgggtg cttggcgacg aaattcggga
      481 cttttacgac ttttgcgccg agtttttcca gcgtttccgc aatcttttcg tcctttagct
      541 ccgcgatttg aaaatgttcc gaagcgcgtt tgaaagcgcc gcgcagacgg tcgagcgcca
      601 gctcgcgctg agccagaacc ttgccgcgcg attgaaattc gccgagcgcg taaagcgtca
      661 ggctttccag ctcgacgctt tgcgttccgc tttcaaaaag agttggctga ttcatccgtt
      721 tccccgctca aacttttaaa attttaacat agctttccct aattattact gtggatttca
      781 acagtcgccc gtgtttcata ccgcaacttt tgccgttata atgtaaatgc tacacttgca
      841 gctgaattta tgagtgattc aaaccccaaa tttcgcgtcg ggctcggcgt tgcgggcggg
      901 attgccgctt acaaagcgat tgaggttttg cgccggctgc aaaaatccgg ctgcgaagtt
      961 caagcggcaa tgacgaaaca cgcgacggaa tttatccagc ctttgacgtt tcgcgctctg
     1021 accgaaaatt acgttctcgt cgacgattac gcgccggaaa atcccgaccc gattgcgcac
     1081 atcaattttt cgcaaaacat cgatttgctg ttgatcgttc cggcgaccgc caatcttctc
     1141 gccaaatttg cgaacggcat cgccgacgat tttctgagct cgacttttct ggcttcgagc
     1201 gcgcccgttt tggttgcgcc cgcgatgaac acgacgatgt ggtttcatcc ggcgacgcag
     1261 cgcaatatcg aaagactgaa aaaagacggc gttctttttg tcgagccggt ttccggcgaa
     1321 ttagcctgca aaaccgtcgg caccggaaaa ctggaagacg tcgaaaatat cgtcgcgcaa
     1381 gccttaaggc ttttgaagtc ggaaatcgga aaccggaagt cggaatcaga gagcgtatct
     1441 ccggacgcag cgccgggcat tcaggactta aaaggcgagc gccttttaat cactacgggc
     1501 ggaacgcgcg aagaaatcga cccggtgcgg ttcatctcca atcattcatc cggcagaatg
     1561 ggctttgctt tagccgaagc ggcgcaaaaa cgcggcgcgc aagtgactgt cgttgccgct
     1621 tcgaccagca tcgcgccgcc ggaaaatgta aaaattacca gcgcgatttc cgccgcagcg
     1681 atgcatcgag ccgtgatgaa cgaattaaac gacgcaaccg tttttgtcgg cgcggcggcg
     1741 gtcgccgatt atcgtccggc aaatcgcgcg gaaaataaaa tcaaaaagac cggagaccgg
     1801 ctcgtcatcg aactggaaaa aaccaccgac attctcgccg aagtttcgaa aaaacgtccg
     1861 tcaggcttaa tcgtcgtcgg ctttgcggcg gagacaaacg acgtcgaggc tcacgcgcgc
     1921 tcgaaaatgc acaggaaaca tctggattta atcgtggcaa acgacgtcag caacgataaa
     1981 acgggtttcg ggtcggaaaa taatcaaggc ttgattttaa gtcgcgacgc gaaaaatccg
     2041 accgagattt cgttgatgag caagcgcgag atggcagaca aaatcctcga cgaagtggta
     2101 aaattcagaa aaagaaatgg ctgaaaacct gcacaacgaa tatggcgcgc tcgtcgccgc
     2161 gctgaaagaa caaatcctgt atctgcagga actcggcgtc gaaaatttta atgtcgattt
     2221 gccggagctc gacccgaaat ttcaaaacgc gggcgcattc cgacgaccgc agaccgaaga
     2281 ccggaaaccg aatctggaaa aattcgtgcc ggacgcgccg gtcgccgcag cgccgccgat
     2341 tgcaaaaacg gaacagccga tcgccaaatc ggaaccgaac acggttcggc agaatctttt
     2401 ggaaaaatca aagctgtcgc gccttccgtc tttgccgaaa cgaggcgcta acgtttccaa
     2461 ttttcaagca gaaagtcaaa tttcagaacc agcaaagagt gacgctatgc caaaacaaaa
     2521 aactaccgcc gaagaaaatc tgaatcaatc gcttttcgga gacattaagc aaacgctgcc
     2581 cgacgcaggc gaaacgctcg aagacattcg actcgacatc ggcaactgca cgcgctgccc
     2641 gctctgggaa ggtcgcacga aaatcgtcca cagcgagggc aacccgcaag ccgatttggt
     2701 ctttgtcggc gaggctccgg gcgccaacga agacgccgaa ggtcgcccgt ttgtcgggaa
     2761 agccgggcag cttttaaaca aaatcatcga agccatcggg atgaagcgcg aagacgtttt
     2821 tatcggcaac atcaatcgct gccgcccgcc gggcaatcgc acgccgacct tgccggaagc
     2881 gcacacgtgc cgcccgtttc tgctgcgcga aatcgccgtc attcgcccga aggtcattgt
     2941 cgttctgggc aacacggcga cgcagaacct gctcgacacg aaaatcggca tcaccaagct
     3001 gcgcggcgaa tttcaagatt attacggcgt caaagtaatg ccgactttcc atccggcata
     3061 tcttttgcgc gacccttcga aaaagcgcga aacgtgggaa gatatgaaaa gagtgcgcga
     3121 ggaaatgaat aaattgaagg gctgacatgg cgttcaaaac tttgttttcg ctcatcaaaa
     3181 ttgttgtaaa atatgagtgg agaaaatatg aaaacgaaaa ttatcgctaa taatgaccag
     3241 gcagtgattg ttttacccag ggaatcgatg caaattttgg gtgttgaggt cggcgacgag
     3301 gtggagtttt caaaagaagc cgatgccgtc gttttgcgct caagttccga agcggaaaga
     3361 aaaaggaagt ttgaaaaagc caaaagagag attttt
//
LOCUS       DASWNE010000530         2524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_688265, whole genome shotgun sequence.
ACCESSION   DASWNE010000530 DASWNE010000000
VERSION     DASWNE010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688265"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VGB68_10505"
     CDS             <1..471
                     /locus_tag="VGB68_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049708.1"
                     /translation="DPVGSSDQRRELLMRSIWVTIRNPWGVGIGNSPIMNDHNLQTHN
                     AYTQVSSELGLLGLIAYVIFIVSPFRKLAAIERTLIAEDKSNWFYYLSIGLQASIAGY
                     MASSFFVSVAYNWFIYYLIAYAVAFRRIYRLENEAKTSEAEQKASLGELQTAQI"
     gene            471..1706
                     /locus_tag="VGB68_10510"
     CDS             471..1706
                     /locus_tag="VGB68_10510"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0049709.1"
                     /translation="MLQITAQIIFWTSVGALFYVYAGYPLLVYLISRAFPKNVRRAAI
                     SPGVTILITAYNEEKDIRRKLENTLQIDYPPEKLEILVASDGSTDGTDEIVRGFSGRN
                     VKLFRQEGRAGKTFTQNKAVERASGEIILFSDATTDYNRDVLQKILPNFADEQVGCVA
                     GKLIYVDDSKSTVGKGAKSYWNYETFLKESESRACSLIGASGCLYAVRKSAYQPMYPE
                     ACSDFLVCTVLFRQGLRSVYESEAVCTEETNRQANKEMQMRVRVISQTFTDLWRNRDM
                     LNPLKSGFYAVQLISHKLLRYAVPAFLALILLTSAALAAQSIFYSLIFLFQTAFYLMA
                     FAAWVLERSGVKLGILAMPLYFVLANIASVIAFYKFLRGERYARWEPIREESSAKTNH
                     REDAEAQRDTAGEMRNAER"
     gene            1742..>2524
                     /locus_tag="VGB68_10515"
     CDS             1742..>2524
                     /locus_tag="VGB68_10515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEY0049710.1"
                     /translation="MSEQKFSQKKIGQKANRSPRVSVIIPAYNVAGFIAETLDSVLAQ
                     DFTGYEIVLVNDGSPDTEKLEKVLEDYFEKIVYVKQENGGTARARNTGIENARGALLA
                     FLDGDDIWLPEYLSGQIKFLTENNLEMVYADAFLFGEVRSAGETYMRKSPSAGAADFE
                     GIVSGRCSVITSGTLVYKQKILEAGMFDAALPRIGMEDFDLWLRLAKAGARIGYQRKI
                     LLKYRVSPNSLSGNSVQRARRNVVGLNIVREKLNLTPSEEKVL"
BASE COUNT          612 a          646 c          678 g          588 t
ORIGIN      
        1 gacccggtcg gttcgagcga ccagcgccgc gaacttttga tgcgttcaat ctgggtgacg
       61 attcgcaacc cgtggggcgt cggtatcggc aattcgccga ttatgaacga ccataattta
      121 cagacgcata acgcttacac gcaggtttcg tcggaactcg gtttgctggg tttaatcgca
      181 tacgtgattt ttatcgtcag cccgtttcgc aagctggcgg cgatcgagcg cacattgata
      241 gcggaagaca aaagcaattg gttttattat ctctccatcg gcttgcaggc gagcattgcc
      301 ggttatatgg cttcgagctt ttttgtttcg gtggcgtata attggtttat ttattatctg
      361 attgcttatg cggtcgcctt tcgccgcatc taccggcttg aaaacgaagc gaaaacaagc
      421 gaagccgaac aaaaggcttc tttaggagag ctgcaaacgg ctcaaatttg atgctgcaaa
      481 ttaccgcaca aattattttc tggacgagcg tcggcgcgct tttttatgtt tacgcgggct
      541 atccgctgct ggtttattta atcagccgcg cgtttccgaa aaacgtcagg cgcgccgcga
      601 tttcgcccgg cgtcaccatt ttaattaccg cctacaacga agagaaagac atccgccgca
      661 agctggaaaa cactttgcag attgattatc cgccggaaaa actggaaatt ctcgtcgcct
      721 cggacggctc gaccgacgga acggacgaaa tcgtccgcgg attttccggt cgaaacgtca
      781 agcttttccg gcaggaaggc agagccggaa aaaccttcac gcaaaacaaa gccgtcgaga
      841 gggcgagcgg cgagataatt ctattttccg acgcgacgac cgattataac cgcgacgttt
      901 tgcaaaaaat cctgcctaat ttcgccgacg agcaggtagg ctgcgtcgcc ggaaaattaa
      961 tttacgtcga cgattcgaaa tcgacggtcg gcaaaggcgc gaaaagctac tggaattacg
     1021 agacgttttt aaaggaaagc gaaagccgag cctgctcgct catcggcgcg tcgggctgtc
     1081 tctacgccgt ccggaaatcc gcttaccagc cgatgtatcc ggaagcgtgt tcggattttc
     1141 tcgtctgcac ggttcttttc cggcagggct tgcgctcggt ttacgaatcg gaagccgtct
     1201 gcaccgagga aaccaaccgg caggcgaaca aggaaatgca gatgcgcgtc cgcgtcatct
     1261 cgcaaacctt caccgacctc tggcgcaacc gtgatatgct gaatccgctg aaaagcggtt
     1321 tttacgccgt ccagctcatc tcgcacaagc ttctgcgtta cgcggttccg gcttttttag
     1381 ctttaatttt attgacgagc gctgctctcg ccgctcaatc gattttttac agcctgattt
     1441 ttctttttca gacggcattt tatttaatgg ctttcgccgc gtgggtgctg gaaagaagcg
     1501 gcgtaaaatt gggaatcctg gcgatgccgc tttatttcgt gctggcaaat atcgcttcgg
     1561 tcatcgcttt ctataaattc ctgcgcggcg agcgttacgc gcgctgggag ccgattcgcg
     1621 aggaatcatc cgcgaagaca aaccacagag aagacgccga ggcgcagaga gatacggctg
     1681 gagaaatgcg taacgcggag cgatgaacgc gaaaattgcc gttcagcgtt tcttttcaat
     1741 tatgtcagag caaaagtttt cacaaaagaa aatcgggcag aaagcgaacc ggtcgccgcg
     1801 ggtcagcgtc attattcccg cgtacaacgt cgccggattt atcgccgaaa ctctggattc
     1861 ggttctggcg caggatttta ccggctacga aatcgtgctc gtcaacgacg gctcgcccga
     1921 cacggaaaaa ctggaaaaag ttctcgaaga ttattttgaa aaaatcgttt acgtcaaaca
     1981 ggaaaacggc ggaacggcgc gggcgcgaaa caccggcatc gaaaacgcgc gcggggcgct
     2041 tttagctttt ctcgacggcg acgacatctg gctgcccgaa tatttaagcg ggcaaatcaa
     2101 atttttgacg gaaaacaatc tggaaatggt ctacgccgac gcctttctct tcggcgaagt
     2161 gcgctcggcg ggcgaaactt atatgcggaa atcgccgtcg gcgggcgcgg ctgattttga
     2221 aggcatcgtc agcggcagat gcagcgtcat cacttccggc acgctcgttt acaaacaaaa
     2281 aattctcgaa gcgggaatgt tcgacgccgc gctgccgcgt atcggaatgg aagatttcga
     2341 tttgtggctg cggctcgcca aagcgggcgc gcgaatcggc tatcagcgaa aaattctgct
     2401 caaataccgc gtcagcccga acagtctttc cggcaacagc gtgcagcgcg cgcgccgcaa
     2461 cgtcgtcggg ctgaatatcg tcagggaaaa attaaattta acgccgagcg aagaaaaagt
     2521 tttg
//
LOCUS       DASWNE010000531         4663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_87599, whole genome shotgun sequence.
ACCESSION   DASWNE010000531 DASWNE010000000
VERSION     DASWNE010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87599"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VGB68_10520"
     CDS             complement(<1..281)
                     /locus_tag="VGB68_10520"
                     /inference="COORDINATES: protein motif:HMM:NF040422.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6438 domain-containing protein"
                     /protein_id="HEY0049711.1"
                     /translation="MRKLKFFAILLIFASCRNAASSRTDFPADSEIPEDLTITLERTG
                     CYATVNVCPTYKLTVKADGAVVFEGKVVTDVKGEIEDKISVEKVKRLVA"
     gene            complement(307..1413)
                     /gene="mqnE"
                     /locus_tag="VGB68_10525"
     CDS             complement(307..1413)
                     /gene="mqnE"
                     /locus_tag="VGB68_10525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HEY0049712.1"
                     /translation="MQFSFDKKLNFIAEKVEEGARLTFDEGMSLFRTDDLNALGKLAD
                     FARRKKHGRTTYFNVNRHFNHTNICVADCKFCGFYKRARQEGAYTHSIEEGIEIAGNA
                     VREGATELHIVGGLNSKLPFRYYTDLFSALKKEFPALHLKALTMVELDFFARFYKMPD
                     EEVIERLKAAGMDSCPGGGAEIFAEPTRSRICDHKCDGARWLELAGKVHRAGLKTNAT
                     MLYGHIESTEDRVDHFVRLREQQDKSGGFQCFIPLAFYPNGTALSSLPGPDAIDNLKT
                     IAVSRLMLDNFDHIKAYWVMLGKATTQTALHFGANDVDGTITDGGELTHSYSVEEGGE
                     QKMTKAELIRMIENAGFEAVERDTVYNRVEKVAA"
     gene            1603..2001
                     /locus_tag="VGB68_10530"
     CDS             1603..2001
                     /locus_tag="VGB68_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049713.1"
                     /translation="MFIRFIVKYFNQQTKRRETGIFRAADYVRDFTAIGATEKENLQK
                     LIEWFDENLPVPEFYDDPTKRSEEQHTYFWFKTSAREFLDRMNALTVILEENGIRVER
                     LTAAEIPGKLIFEDECQIAVTPLGDVLKIK"
     gene            complement(2124..2852)
                     /locus_tag="VGB68_10535"
     CDS             complement(2124..2852)
                     /locus_tag="VGB68_10535"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HEY0049714.1"
                     /translation="MKIPTLQGIIKRRILVNYRAEQSVIQKILPAGFRPKLHKGNAIA
                     GICLIRLEHMRLKHTPEFVGIDSENAAHRIAVLWEDASGETKEGVYIPRRDTDSFINS
                     AVGGRLFPGEHHRANFAVGENGNKINFSMKSADEIASVKLAGTISKNLPENSVFASLA
                     EASGFFEKGSLGYSVTKNGTNLDGINLEIEKWKVEALEVDFVESSFYDDERIFPKGSI
                     EFDHALLMRNVEHRWHGAPSFELN"
     gene            complement(2849..3727)
                     /locus_tag="VGB68_10540"
     CDS             complement(2849..3727)
                     /locus_tag="VGB68_10540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HEY0049715.1"
                     /translation="MKSFRQKFKTALEMIKFEHTLFALPFAFLGAVLAADGLPSWRQI
                     LWITAAMFGARSAAMTFNRIVDRRFDAQNPRTANRELPSGKLSLEFAWSFFIASATLF
                     LLAAYSLNWLTFALSPVALLSILGYSYAKRFTSLAHLILGWSLAISPTAAWIAVRGAI
                     DSETPLLLSLLVMMWTAGFDVLYACQDFEYDKKAGLRSIPARFGIKNSLWIARIFHAQ
                     AFVVLLLFYLVTDLGWLALTGVAAVGALMIYQHTLVKPNDLSRMNAAFFTTNAFVSVI
                     LFVTFGGAVFMEKFLK"
     gene            3938..4471
                     /locus_tag="VGB68_10545"
     CDS             3938..4471
                     /locus_tag="VGB68_10545"
                     /inference="COORDINATES: protein motif:HMM:NF021503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein"
                     /protein_id="HEY0049716.1"
                     /translation="MPSGKTHDAITFLLIAPTFAAAWAATKNIPVAGIVTFGMLFGGL
                     MFGPDLDTVSRQYSRWSVFRFLWYPYQVFFKHRSRWSHGLLFGTFLRVVYFLGALTLT
                     AFLVSYLVTAYRGEGAPDLLEMTKTWSAIGEYVRANAGEYAFASLFVGLWLGAASHTF
                     TDMAGSFIKTGRITEFL"
BASE COUNT         1188 a         1220 c         1127 g         1128 t
ORIGIN      
        1 tccgcaacca gccgctttac cttttcgacg ctgattttat cttcgatttc accttttacg
       61 tccgttacga cttttccttc aaaaacgacc gcgccatcgg ctttaaccgt caatttataa
      121 gtcgggcaaa cattcaccgt cgcataacag ccggttcttt caagcgtaat cgtcaaatct
      181 tcgggaattt cggaatcagc cggaaaatcc gtccgagaag aggcggcatt tctgcaggaa
      241 gcgaatatca ataaaatcgc aaaaaatttc agtttcctca tttcaaaaag ttctctgagc
      301 aaaatttcac gccgcgactt tttccacgcg attataaacc gtgtcgcgct cgaccgcttc
      361 aaagcccgcg ttttcaatca tcctgatcag ctcggctttc gtcattttct gttcgccgcc
      421 ttcttcgacc gaatagctgt gcgtcagctc gccgccgtcg gtaatcgtgc cgtcgacgtc
      481 gttggcgccg aaatgcagcg cggtctgcgt cgtcgctttg ccgagcatca cccaataggc
      541 tttgatgtgg tcgaaattat cgagcatcag gcgcgagacg gcgattgttt tcagattatc
      601 aatcgcgtcc ggtcccggca ggctcgataa agccgtcccg ttaggataaa acgcgagcgg
      661 aataaagcat tgaaaaccgc cggatttgtc ctgctgctcg cgcagcctga cgaaatggtc
      721 aacgcggtct tccgtggatt cgatgtgacc gtagagcatc gtggcgttgg ttttcagacc
      781 ggcgcgatgg acttttccgg caagctccag ccagcgcgcg ccgtcgcatt tgtggtcgca
      841 gatgcgcgag cgcgtcggct cggcgaaaat ctccgcgccg ccgcccggac acgagtccat
      901 tcccgccgct ttcagccgct cgataacttc ctcgtccggc attttgtaaa aacgcgcgaa
      961 gaaatccagc tcgaccatcg tcaacgcttt cagatgcaaa gcgggaaatt cttttttcag
     1021 agccgaaaac aaatcggtgt aataccggaa aggcagtttc gaattcaagc cgccgacgat
     1081 atgcagctcc gtcgcgccct ctctgacggc gtttccggct atctcgattc cttcttcaat
     1141 cgaatgcgtg tacgcgcctt cctgacgcgc gcgtttgtaa aaaccgcaga acttgcagtc
     1201 ggcgacgcag atgttcgtgt gattaaaatg acgattgacg ttgaaataag tcgtccgtcc
     1261 gtgttttttc cgccgcgcga aatcggcgag cttgccgagc gcgttcaaat cgtcggtgcg
     1321 gaaaagcgac attccctcgt caaacgtcaa acgcgcgcct tcttcgactt tttcagctat
     1381 aaaattcagt ttcttatcaa atgaaaattg catagtattt atgatagagg gcaatccttg
     1441 tgccttaact ttaacaaaaa cgcgggaaaa tgtttatccg gtaagcccgg taaacaggcg
     1501 gtttatttga cgggaaatat tttcgggttt aacttaaaca gcaaaggcgc aggcaatttg
     1561 caaaaaataa agccggatta ggaaaaatgc gaaaacccga aaatgttcat ccgttttatt
     1621 gttaaatatt ttaatcagca gacgaaaagg cgcgagaccg gcatctttcg ggcggcggat
     1681 tacgttcggg attttacggc aatcggcgcg acggaaaagg aaaatctgca aaaacttatc
     1741 gaatggttcg acgaaaatct gcccgtgccg gagttttacg acgacccgac aaaaagaagc
     1801 gaggaacagc acacctattt ctggtttaag acgagcgcgc gcgaatttct cgacagaatg
     1861 aacgcgctga ccgtaatttt agaagaaaac ggaatccggg tggaaagatt aaccgccgcc
     1921 gaaataccgg ggaaattgat tttcgaagac gaatgccaga tagccgtcac accgctcggc
     1981 gacgttttaa aaatcaagtg acaaaaacct gccggcggca aaagacgcag cgattcggtc
     2041 aaacgccagg cgcccgcttt tttcgacgtt tgcggcgatt cattttaatt tctcgccaaa
     2101 tgaaaaccgc gcaaattcaa aattcaattt aattcaaaac tcggcgcgcc gtgccaacgg
     2161 tgctcgacgt ttcgcatcag aagggcgtgg tcaaattcga ttgaaccttt cggaaatatc
     2221 ctctcatcgt cgtaaaaact cgattcgaca aaatccacct cgagcgcttc gactttccat
     2281 ttttcgattt ccagattgat tccgtctaaa ttcgttccgt ttttcgtcac ggaataaccg
     2341 agcgagcctt tttcaaaaaa accggaggct tcggcaagcg aagcgaaaac ggaattttcc
     2401 ggaagatttt tagaaatcgt gcccgccagt ttaaccgaag cgatttcgtc cgccgatttc
     2461 atcgaaaaat ttattttgtt tccgttttcg ccgacggcaa aattcgctct gtggtgctcg
     2521 cccggaaaaa gtcttccgcc gaccgccgaa ttgataaaag aatccgtatc gcggcgcgga
     2581 atataaacgc cttcttttgt ttcgccgctc gcatcttccc acaagacggc gattcgatgc
     2641 gcggcgtttt cgctgtcgat gccgacaaac tccggcgtat gcttcagacg catatgttcg
     2701 agccggatta aacaaatacc cgcaattgcg tttcctttgt gcaatttagg gcgaaagccc
     2761 gctggcagaa ttttttgtat tacgctttgt tcggcgcggt aatttactaa aattcttcgt
     2821 ttaataatgc cttgcagcgt cggaattttc atttcagaaa cttctccata aaaaccgcgc
     2881 cgccgaaggt gacgaataaa atcacgctga cgaaggcgtt ggtcgtaaaa aacgccgcgt
     2941 tcattcgtga taaatcgttc ggcttgacaa gcgtgtgctg gtaaatcatc agcgcgccga
     3001 ccgccgccac gccggttaaa gcgagccagc ctaaatcagt caccagataa aacaaaagca
     3061 aaacgacgaa cgcctgcgcg tgaaaaattc tggcaatcca gagcgaattt ttgatgccga
     3121 aacgcgccgg aatcgagcgc agcccggctt ttttgtcgta ttcaaaatcc tgacacgcgt
     3181 ataaaacgtc gaatccggca gtccacatca tcaccagcag cgacagaaga agcggagttt
     3241 ccgaatcaat cgcgccgcgc acggcaatcc aggcggcggt cggcgaaatc gccaggctcc
     3301 agcctaaaat cagatgcgcg aggctcgtaa atcttttcgc gtaggaataa cccaaaatgc
     3361 tcaaaagcgc gacgggcgac aaggcaaacg tcagccagtt cagcgaataa gcggcaagca
     3421 gaaacaacgt cgccgacgcg atgaaaaacg accaagcaaa ctcgagcgaa agctttccgc
     3481 tcggcaattc gcggtttgcc gtgcgcggat tttgggcgtc gaagcggcgg tcgacaatgc
     3541 gattaaaagt catcgccgcg cttcgggcgc caaacattgc cgccgttatc cagagaatct
     3601 gccgccacga cggcaaaccg tctgccgcca gaaccgcgcc gagaaaagcg aaaggcaagg
     3661 caaataaagt atgctcgaac tttatcatct cgagcgcggt cttaaatttt tgccggaaag
     3721 atttcatcgc gtttcaaatc ccgcattgcg tgtaagcaaa cgcagtgccg aaagcaaagt
     3781 ttagcaaaat cgagatattt ttctagcttc gtttggcaat ttgcaaaatt ctcccgcaga
     3841 aaattgcaaa ttactgtaat atatttttcg tccgcttgcc gcaagcttta tcgagagcgg
     3901 caaataaaag aacaatctga aatcgcaaat ccttcttatg ccttcgggaa aaactcacga
     3961 cgccattaca tttctgctca tcgcgccgac tttcgccgcc gcgtgggcag cgacgaaaaa
     4021 tattccggtc gccgggatag tcactttcgg aatgcttttc ggcggtctga tgttcggtcc
     4081 cgatttagac accgtgagcc ggcagtattc gcgctggagc gtttttcgct ttctctggta
     4141 cccgtatcag gtgtttttca aacaccgctc gcgctggtcg cacgggctgc ttttcggcac
     4201 cttcctgcgc gtcgtctatt ttctgggcgc gctgacgctg acggcatttc tggtcagcta
     4261 tctggtaacg gcttaccggg gcgaaggcgc gccggattta ttggaaatga cgaaaacctg
     4321 gagcgcaatc ggcgaatacg tccgcgcaaa tgccggcgaa tacgcctttg cgtcgctgtt
     4381 cgtcggatta tggctcggcg cggcgagtca tacttttacc gatatggcag gctcatttat
     4441 caaaacgggc agaattacag aatttttata attttagatt tttagattga actcgcaaat
     4501 ttctatccgc ccttgcgggc taatgaaatg cgcgaagcct gacgccggac ggcgtcggac
     4561 acgtttcgat ttttgctcaa agcaatcaaa tctctgagct gcaaacgcgg caaaatactc
     4621 attacgctcg acagcggcat ccgcggattt cgcgccagat tgt
//
LOCUS       DASWNE010000532         6847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_265877, whole genome shotgun sequence.
ACCESSION   DASWNE010000532 DASWNE010000000
VERSION     DASWNE010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6847
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265877"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /locus_tag="VGB68_10550"
     CDS             <1..1138
                     /locus_tag="VGB68_10550"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0049717.1"
                     /translation="AEATKDYGEKLAKTEIGSADEAIIIYTSGTTGKPKGCLLTHGNL
                     IANARQITEWLGFDENDRLLTIMPLFHMNAVSVTTMTALYAGASTVVSPKFSASRFWQ
                     IVSDYKITSFGSVATMLSMLLQKSAQPSAESRKPEAEDRRPKSLRFAMCGSAPVPAEV
                     LRKFEETFDCLVIEGYGLSESTCRSTFNPPDKRRRPGSCGQPIGNEMKVFDENEQEAP
                     NGELGEIVLRGENIFKGYFKNEEATRKAFQNGWFHTGDIGYRDADGFFYIADRKSDMI
                     IRGGENIYPREIDELLYTHGAVSSAAVIGVPDELYGEEVAAFVVLKDDGQASEEDLIN
                     FCKQHLADYKCPKRIYFVADIPKGPTGKLLKRELARIYSENAQR"
     gene            complement(1182..2384)
                     /locus_tag="VGB68_10555"
     CDS             complement(1182..2384)
                     /locus_tag="VGB68_10555"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY0049718.1"
                     /translation="MAEKKVREMRLSARRVFLDPSTPPVLSIVRIVVITLLILAVADF
                     AKGIIVSLTHLFFMVILAVFFAYLIEPLVKLIRQPFEERQRERFMPRPLAIAVAYILV
                     FSILGIAIAWLAPLVNEQAKQLTANLPTYTSSIQSWVRDISTRFDRYRIPEAIQTQIN
                     EKAGGVAADIGTQVTNFLIALASYLPWLILVPVLGFFFLKDANFFRISFLRLFPSGRW
                     RSRIESVIYDVNQTLAAYTRAQLISCVLIGVICTIGFYLIGINYALLLGIVAGVLEFI
                     PLLGPITVGIIAVTAAGLQSPWQALYTFIFLAALRIVHDYFTYPRIVRGGIHLHPLAI
                     ILSVLAGEQVAGIPGVFISIPVVALLTVLYKHVLEHSGSKGLFTGLFEEKADESKNAD
                     ETGEQKTA"
     gene            complement(2396..2905)
                     /locus_tag="VGB68_10560"
     CDS             complement(2396..2905)
                     /locus_tag="VGB68_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049719.1"
                     /translation="MNTDKNRFIFGFKARVSGFCLLGCLFLSACQTAVTPVGRLQTPT
                     PSPTPEVKEAKDDFAEKLEYVQKGAFSFVYVFRRKDGAALDSDDSKYLRANSPLETNY
                     WVVTDDRKVAIAGSNYQFLPEALDALKKRFNVEDLSKPSEANPEANSNVNDKANSKAG
                     NKANSNAGN"
     gene            complement(2913..4751)
                     /locus_tag="VGB68_10565"
     CDS             complement(2913..4751)
                     /locus_tag="VGB68_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049720.1"
                     /translation="MEEIQTENSAVVEKPVEPAAVRQSWKEWLGTEQAVRWVYLVFGL
                     CAIFLLMIVLQRSTDAICCGDWDGYYHIRWSSLLWESFKSGHWLPEFKWLPLTVLNPQ
                     DYNDHHFLFHLLQIPFLWVFEPVMAAKVATVFFAGLAIFSVYWLMFYYKVDYLLVWLA
                     VLLTCANPFFYRMNMAKAPPLTIIITVIGIYLLFERKYVWLLPLMFLFVWTYSLFPLL
                     FFAAVIWAAIVGWNERRLEWKPVAYTTGGMILGNIINPYFPSNLTLFYEHFVTKIKVG
                     GDFAVAVGGEWYPYSGQDLLTHFPIAMTAMLIGYILFMPKNGKLPEKATFFLVFVTIL
                     LASQFRSKRFAEYFPPFAVLFAAFSWQAFLVPNTLQLPEDFKKDIEPYLDKKRSEREE
                     LYEKIKQAIAAVIGVLLVVLIFYNFRGVHIDWWGLKIDQEGLTESIRNNEPHDRYRRA
                     ADWLNQNVPENEIIFNCNWDDFPKLFFFDTKHRYVYGLDPNYLYSKNPELSKLIGEIT
                     GGKIDDPGPVIREKFGARYVFSDAKENTDFLAKALESGWFEMAYEDDEAYILKIRDEK
                     GEPPNASAIDDKNGNADEPTEEELKQLEEEEKAADANRDAAEEEEK"
     gene            5020..5655
                     /locus_tag="VGB68_10570"
     CDS             5020..5655
                     /locus_tag="VGB68_10570"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0049721.1"
                     /translation="MYPQGNLFIPAPHGRLEAILKEPDGAAKGAALVCHPHPMGGGTM
                     HNKVVYRAAAGLLEAGLAALRFNFRGVGLSTGEHDEGRGEKEDVRAALDYLTENYARQ
                     EITFAGFSFGSRFGTETVLTDARVPRLISIGTPVDKYDYSFLRACKKPILFVHGDADE
                     FGSVESLKKLYDQLECEKELTIFENCGHFFDRHLNELKNAVRDWTTKQIQS"
     gene            5704..6117
                     /locus_tag="VGB68_10575"
     CDS             5704..6117
                     /locus_tag="VGB68_10575"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HEY0049722.1"
                     /translation="MENEQLENQTPVIDKTREVAIRLTMMPRDTNAHGTVFGGIILSY
                     IDVAGGVEAVRHTGHDRFVTVAMKEVIFLEPVFIGDLVSFYAETVKVGKTSITVHVAV
                     EAERFGSHGQSVRVTEADVTYVAINQYREKVLIGK"
     gene            6200..>6847
                     /gene="truA"
                     /locus_tag="VGB68_10580"
     CDS             6200..>6847
                     /gene="truA"
                     /locus_tag="VGB68_10580"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEY0049723.1"
                     /translation="MPTWKLEIEYEGTRYRGWQIQHNAKTVQGELMTAARQLFASKVE
                     IGGSGRTDAGVHALRQIAHLKSFELKTNVTPRQIQHGFNDLLPHDINILKVSNAPENF
                     HARHDAVARYYLYQISTRRTAFGKNYVWWIKDKLNFGEMQEAAAMLVGKHDFRSFSES
                     SEEEKSTLVKVERAEVFTDGDLICFRIGASHFLWKMVRRIVGMLAEAGRGNLSSAE"
BASE COUNT         1777 a         1768 c         1769 g         1533 t
ORIGIN      
        1 agcggaggcg acgaaagact acggcgaaaa attggcgaaa acggaaatcg gcagcgccga
       61 cgaagcgatt attatttaca cttccggaac gaccggaaaa ccgaaaggct gtcttttgac
      121 acacggaaat ttaatcgcca acgcgcggca aatcaccgaa tggctcggct ttgacgaaaa
      181 cgaccgttta ttgacgatta tgccgctttt tcatatgaac gccgtttccg tgacgacgat
      241 gacggcgctc tacgcgggcg cgagcacggt tgtcagcccg aaattttccg cctcgcgttt
      301 ctggcaaatc gtttccgatt acaaaatcac ttcgttcggc tcggtggcga cgatgctttc
      361 gatgcttttg cagaaaagcg ctcagccgtc cgccgaaagc cgaaagcccg aagccgaaga
      421 ccgaagaccg aaatcgcttc gatttgcaat gtgcggctcg gctccggttc cggcggaagt
      481 tttgcgaaaa tttgaagaga ctttcgattg cctggtcatc gaaggttacg gtttgagcga
      541 atcgacctgt cgctcgacct ttaatccgcc ggataaacgg cgtcgtcccg gctcgtgcgg
      601 tcagccgatc ggcaacgaaa tgaaggtttt tgacgagaac gagcaggaag ctccgaacgg
      661 cgagctgggc gaaatcgtcc tgcgcggcga gaatattttt aaaggttatt ttaaaaacga
      721 agaggcgacg cggaaagcgt ttcaaaacgg ctggtttcac acgggcgaca tcggttatcg
      781 cgacgcggac ggttttttct acatcgccga ccgcaaatcc gatatgatta ttcgcggcgg
      841 cgaaaacatc tacccgcgcg aaatcgacga gcttttatac acgcacgggg ctgtttcgtc
      901 ggctgccgtc atcggcgtgc cggacgaatt atacggcgag gaagtcgccg cgttcgtcgt
      961 tttaaaagac gacgggcagg cgagcgagga agatttaata aatttctgta aacaacatct
     1021 ggcggattac aaatgtccga aacgaattta tttcgtcgcc gacattccga aaggtccgac
     1081 cggaaaactg ctcaagcgcg agctggcgcg gatttacagc gaaaacgcgc aacgatgaat
     1141 tgtcggttca tcgttgcgcg ttcataattc atcgttttcc atcacgccgt tttctgctcg
     1201 cctgtttcgt ccgcattttt gctctcgtcc gctttttcct cgaacaatcc ggtaaaaagc
     1261 cctttgctgc cgctgtgttc gagaacgtgt ttataaagga cggttaagag cgcaacgacc
     1321 ggaatcgaga taaaaacgcc cggaattccc gcgacctgct cgcccgccag aaccgacagg
     1381 ataatcgcca gcgggtgcag gtgaatgccg ccgcgcacga tgcgcgggta ggtaaaataa
     1441 tcgtgaacga tgcgcagagc ggcgagaaat ataaaagtat aaagcgcctg ccacggcgat
     1501 tgcagcccgg cggcggtaac ggcgatgatg ccgaccgtga taggtccgag cagcggaata
     1561 aattccagaa ctccggcgac gatgccgagc agtaaagcat aattgatgcc gatcagataa
     1621 aaaccgatgg tgcagataac gccgataaga acgcaggaaa tcagctgcgc gcgcgtgtaa
     1681 gccgccagcg tctgattgac gtcgtagatg accgattcaa tccgcgagcg ccagcgcccg
     1741 ctcggaaaaa gccgcagaaa tgagatgcgg aaaaaatttg cgtctttcag gaaaaagaaa
     1801 ccgagcaccg gaacgaggat gagccacggc agatacgaag ccagagctat caaaaaattt
     1861 gtgacctgcg tcccgatgtc ggcagccacg ccgcccgcct tttcgttaat ctgagtttga
     1921 atcgcttcgg gaatacgata acggtcgaaa cgagtgctga tgtcgcgaac ccacgattga
     1981 atcgagctgg tgtaagtcgg cagattggcg gtcaattgct tcgcctgctc gttgaccagc
     2041 ggcgcgagcc aggcaatggc aatgccgaga atcgaaaaaa cgagaatata ggcgacggca
     2101 atcgccagcg ggcgcggcat aaatctttct cgctggcgct cttcgaacgg ctggcgaatc
     2161 agcttgacca gaggctcaat caaatacgcg aaaaagactg ccagaatcac catgaaaaaa
     2221 agatgcgtca gcgatacgat aatgcctttc gcaaaatcgg cgaccgccag aatcaggagc
     2281 gtgatgacga cgatgcgaac aatcgaaagc accggcgggg tcgacgggtc gagaaaaacc
     2341 ctgcgcgccg ataatctcat ttcacggact ttcttttctg ccacaagctt tctccttaat
     2401 tgccggcgtt tgaattcgct ttattgcctg cctttgaatt cgctttatcg ttaacgttcg
     2461 aattcgcttc gggattcgct tccgaaggct tcgataaatc ctcgacgtta aagcgttttt
     2521 tcagagcgtc cagagcttcc ggcagaaact ggtaattcga accggcaatc gcgactttgc
     2581 ggtcgtcggt aacaacccag taatttgtct cgagcggcga atttgcccgc aggtatttgc
     2641 tgtcgtcgga atccaaagcc gccccgtctt tccgccggaa aacgtaaacg aaagagaaag
     2701 cgcccttttg aacgtattcc agtttctcag cgaaatcgtc tttcgcttcc ttaacttcgg
     2761 gcgtcggcga cggcgtcggc gtctggagcc tgccgaccgg cgtgacggcg gtctgacagg
     2821 ctgagaggaa caaacagccg agcagacaaa aacccgagac cctcgcttta aaaccgaaaa
     2881 taaatctgtt tttatccgtg ttcatcggcg gcttattttt cttcctcttc agccgcgtcc
     2941 cggttggcgt cagcggcttt ttcttcctct tcaagctgtt tcagctcttc ttcggtcggc
     3001 tcgtccgcgt ttccgttttt gtcgtcgatg gctgacgcgt tcggcggctc gcctttttcg
     3061 tcgcggattt ttaaaatata cgcttcgtcg tcttcataag ccatttcaaa ccaaccgctt
     3121 tccagcgctt tggcgagaaa atcggtattc tctttggcgt cggaaaagac atagcgcgcg
     3181 ccgaattttt cgcggatgac aggtcccggg tcatcgattt ttccgcccgt gatttcgccg
     3241 atgagcttcg agagctccgg atttttcgaa tacaggtaat tcgggtcgag cccgtaaacg
     3301 tagcgatgtt tcgtgtcaaa gaaaaacagc ttcggaaaat cgtcccagtt gcagttaaag
     3361 attatctcgt tttccggcac gttctggttg agccagtcgg cggcgcggcg atagcggtcg
     3421 tgcggttcgt tgttgcggat cgattcggtc aagccttcct ggtcgatttt caagccccac
     3481 caatctatat gaacgccgcg gaaattgtaa aatatcagga cgaccagcaa aacgccgatg
     3541 accgcggcga ttgcttgttt gattttttcg taaagctcct cgcgttcgct tcgtttcttg
     3601 tccagataag gctcgatgtc ttttttgaaa tcttccggca gctgcaaagt gttcggaacc
     3661 agaaacgcct gccagctaaa ggcggcgaac aaaaccgcaa acggcggaaa atattcggca
     3721 aagcgtttcg agcgaaactg cgatgccagc agaatcgtga cgaaaaccag aaaaaacgtt
     3781 gccttttcgg gcagttttcc gtttttcggc ataaacagga tgtagccgat aagcatcgcc
     3841 gtcatcgcaa tcggaaaatg cgtcagcaaa tcctgtcccg aatacggata ccattcgccg
     3901 ccgacggcga cggcaaaatc gccgccgact tttattttcg tgacgaaatg ctcgtagaaa
     3961 agcgtcaggt tactcggaaa atacgggttg atgatgttgc ccagaatcat tccgccggtc
     4021 gtatacgcga ccggcttcca ttccagcctg cgctcgttcc agccgacaat cgccgcccag
     4081 atgaccgccg cgaaaaacaa aagcggaaaa aggctgtaag tccagacaaa caaaaacatt
     4141 agaggcagca gccagacgta ttttcgctcg aaaagaagat aaattccgat gaccgtgatg
     4201 ataatcgtca gcggcggcgc tttcgccata ttcatccggt aaaaaaacgg gttggcgcac
     4261 gtcaaaagca cggcgagcca gaccagaaga taatcgacct tataataaaa catcagccag
     4321 taaaccgaaa aaatcgctaa accggcgaag aaaaccgtcg ccacttttgc cgccatcacc
     4381 ggctcgaaaa cccacaaaaa aggaatctgc aaaagatgaa aaagaaaatg atggtcgttg
     4441 taatcctgcg ggttcagaac cgtcagcggc agccatttaa attccggcag ccagtgaccg
     4501 cttttaaagc tttcccacaa aagcgagctc cagcggatat gataataacc gtcccaatcg
     4561 ccgcagcaaa tcgcgtcggt cgagcgctgc aaaacaatca tcagcagaaa gatggcgcat
     4621 aacccgaaaa ccagataaac ccagcgcacg gcttgctccg tgccgagcca ctccttccac
     4681 gactgcctga ccgccgcagg ttcgaccggc ttttcgacga ccgccgaatt ttccgtttgt
     4741 atttcttcca ttttcgataa aaacgagtgc gtaatatttg aattaatctt agaaaaaata
     4801 tcacgaaacg acgttaaact tctaatgacg cgccgattaa ttacgaatta cgaattacga
     4861 attacgaatt ggaagaaacg accttcgggc ttgaaacttt gacccttgaa aatattcaac
     4921 cttcaaaaat caaaaatcat ccgttcgtaa ttcgtaattc gtaattgaat tccgcatttg
     4981 ccaatcgcag cccgcaaatc taaaatcgaa aattcaacta tgtatccgca aggcaacctg
     5041 tttattcccg cgccgcacgg acggctggaa gcgattttaa aagaaccgga cggcgcggcg
     5101 aaaggcgcgg cgctcgtctg ccacccgcat ccgatgggcg gcggcacgat gcacaacaag
     5161 gtcgtttacc gcgccgccgc cggactgctc gaagccggtc tggcggcgct tcgctttaac
     5221 tttcgcggcg tcggactttc gaccggcgag cacgacgaag ggcgcggcga aaaagaagac
     5281 gtccgcgcgg cgctggatta tttaacggaa aattacgcgc ggcaggaaat cacgtttgcc
     5341 gggttttcgt tcggctcgcg cttcggcacg gaaaccgtct tgacggacgc gagagttccg
     5401 cgtcttatca gcatcggaac gccagtcgat aaatacgatt attcgttttt gcgggcgtgt
     5461 aaaaaaccga ttttattcgt tcacggcgac gctgacgagt tcggctcggt ggaaagctta
     5521 aaaaaacttt acgaccaatt ggaatgcgaa aaagagttaa ctatttttga aaactgcggg
     5581 cattttttcg accggcatct gaacgaactg aaaaacgccg tccgcgattg gacgacgaag
     5641 caaattcaaa gttaatttcc gcggcaaaac atttgtttcg cctgtttcgc cggcaaaatc
     5701 tttatggaaa acgaacaact cgaaaatcaa actccggtaa tcgacaaaac gcgcgaagtg
     5761 gcgattcgcc tgacgatgat gccgcgcgac acgaacgcgc acggcacggt cttcggcggc
     5821 attatcctga gctatatcga cgtcgcgggc ggcgtcgaag ccgtgcgcca cacgggacac
     5881 gaccgctttg ttacggtggc gatgaaggaa gtgatttttc tggagccggt ttttatcggc
     5941 gatttggtga gcttttacgc cgaaaccgta aaggtcggaa agacttcgat tacggttcac
     6001 gtcgccgtcg aagccgagcg tttcggctcg cacggacaga gcgtgcgcgt caccgaagcc
     6061 gacgtaactt acgtggcgat taaccaatac cgcgaaaaag ttttgattgg gaaatagcgt
     6121 ataactgata attgataatt gataactgat agtttgtttt tcactatcaa ttatcaatta
     6181 tcaactgtta actaataaaa tgccgacttg gaaactggaa atcgaatatg aaggcacgcg
     6241 ttatcgcggc tggcaaatcc agcataatgc caagaccgtg cagggcgaat tgatgaccgc
     6301 cgcgcggcag ctttttgctt caaaagtcga aatcggcggc tcgggcagaa ccgacgccgg
     6361 cgttcacgcg ctcaggcaaa tcgcgcattt gaagtctttt gaattgaaaa ccaacgtcac
     6421 gccgcgccag attcagcacg gctttaacga ccttctgccg cacgacatca acattctgaa
     6481 agtctccaac gcgccggaaa attttcacgc gcgccacgat gccgtcgccc gctattacct
     6541 gtatcagatt tcgacgcgcc ggacggcttt cggcaaaaat tacgtctggt ggataaaaga
     6601 taaattaaat tttggggaaa tgcaggaagc cgccgcgatg ctcgtcggca aacacgattt
     6661 tcgctctttt tcggaatcat ccgaagaaga aaaatcgacg ctcgtcaagg tcgagcgcgc
     6721 cgaagttttc accgacggcg atttgatttg ctttcgcatc ggcgcgagcc attttttgtg
     6781 gaagatggtc agaagaattg tcggaatgct ggcggaagcc ggacgcggaa atttatcgtc
     6841 cgccgag
//
LOCUS       DASWNE010000533         1855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_476465, whole genome shotgun sequence.
ACCESSION   DASWNE010000533 DASWNE010000000
VERSION     DASWNE010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476465"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1494
                     /locus_tag="VGB68_10585"
     CDS             <1..1494
                     /locus_tag="VGB68_10585"
                     /inference="COORDINATES: protein motif:HMM:NF015368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049724.1"
                     /translation="TDICGQQISPLWGELPNGKYGVGYKTLIERDGSRSFPKKSGASF
                     ISGARQIKISLWYPAQNSGKPKMKFREYLSALEDAASIPEKSESRSRFMQFSVLQELP
                     EAAIANLLAVSTAATRNAPFLKGEKFPLVILGQGLYYESPATHTILCEFLASQGFIVA
                     TTDLVGAHSPFVKLDAVDLEADVRDLEFVISRARLLEGADKEKLAVAGFDMGGLAGLI
                     LTMRNREVDAFASLDSGIMAEHNLRLVKATADYDALRLGVPLFHATRPAEELARFGVS
                     EDTSFLDAARFSDRLVLRFPGARHADFTSRPMIEFIAAQMRDEATTRRRLSFEAAARN
                     LSAFLEAHLKNDRQALEFLYSQAVDEPPAKIKFKKERKAATRRPTFTEDEFLNYLYSE
                     GAEKASNLYRKLRAEFPNERIFRESTLKQIGYNRFYRGEASEAIGIFKLNVEAFPDSS
                     NAYETLAQAYALSGDLEAAKSNYKKSLQLDPNNENAKTQLKRLEEKK"
BASE COUNT          463 a          451 c          501 g          440 t
ORIGIN      
        1 acagacattt gcgggcagca aatctcgccg ctgtggggcg aattgccgaa cggaaaatac
       61 ggcgtcgggt ataaaactct aattgagcgg gacggctcgc gctcttttcc gaaaaaatcc
      121 ggcgcctctt ttatttccgg cgcgcggcaa atcaaaatca gcctctggta cccggcgcaa
      181 aattcgggaa agccgaagat gaagtttcgc gaatatctat cggcgctcga agacgcggcg
      241 agcattccgg aaaaatcgga gagccgttcg agatttatgc agttttcagt cttgcaggag
      301 cttcccgaag cggcgattgc gaatttactc gccgtttcga ccgccgcgac ccgaaacgcg
      361 ccgtttttaa aaggcgaaaa atttccgctg gtgattctcg gtcaggggct ttattacgaa
      421 tcgccggcga cgcacacgat tctctgcgaa tttctggcga gtcagggttt tatcgtagcg
      481 acgaccgatt tagtcggcgc gcattcgccg ttcgtcaagc tggacgcggt cgatttggaa
      541 gccgacgtgc gcgatttgga attcgtcatc agccgcgcgc ggcttttgga aggcgcggac
      601 aaagaaaaat tagccgtcgc cggattcgat atgggcgggc ttgccggttt gattttaacg
      661 atgcgaaacc gcgaggttga cgcctttgcg agcctcgaca gcggaattat ggcggagcat
      721 aacctgcggc tcgtcaaagc gacggcggat tatgacgcgc tgcgcctggg cgtgccgctt
      781 tttcacgcga cgcgtccggc tgaagagctt gcgcgctttg gcgtcagcga agatacgagc
      841 tttctcgacg cggcgcgttt ttccgaccgt ctggttttgc gatttccggg cgcgcggcac
      901 gccgatttca cctcgcgccc gatgattgaa tttatcgcgg cgcaaatgcg ggacgaagcg
      961 acgacgcggc gaagactttc ttttgaagcg gcggctcgga atctgtcggc ttttctcgag
     1021 gcgcatctga agaatgaccg gcaggcgctg gaatttcttt actctcaggc agtcgacgaa
     1081 ccgcccgcga aaattaaatt caaaaaagag cggaaagccg cgacccgtcg cccgacgttt
     1141 acggaagacg agtttttaaa ttatctttat tcggaaggcg cggaaaaagc ctcgaatctt
     1201 tatcgaaagc tgcgagccga gtttccgaac gagcggattt ttcgcgaatc gacgcttaaa
     1261 cagattggct acaatcgttt ttaccgcggc gaagcgagcg aggcaatcgg catcttcaaa
     1321 ttaaacgtcg aggcgtttcc cgattcgtcg aacgcttatg aaactctggc gcaagcctac
     1381 gctttgagcg gcgacctcga agcggcaaaa tcgaattaca aaaaatcttt gcagctcgac
     1441 ccgaacaacg aaaacgcgaa aacgcagtta aaacgattgg aagaaaaaaa ataatctccc
     1501 ggattttccg gcgcgaaaca agttaattta cagttctgaa aacttaattt acagctcctg
     1561 acgcataatt tataaagctt aaaaacgact ttacagttcc tgaaatttga tttaaggctc
     1621 agctaaaccg ggctttatgt ttattaaatc gggcattgcg aacccggaat agagttttat
     1681 gaatcttaaa ggaaatttac cgaaccttaa atcgagtttt atcgacctta aactttgttt
     1741 gctaatgaga aaatatgtcg gcggctgagc gcgcgaaaaa aagaaataaa ataaattggc
     1801 ggcacttcgg cgtcgggttt ctggtctgga cgattgtcgg gctttcgttt gccct
//
LOCUS       DASWNE010000534         2243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_614374, whole genome shotgun sequence.
ACCESSION   DASWNE010000534 DASWNE010000000
VERSION     DASWNE010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614374"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VGB68_10590"
     CDS             complement(<1..266)
                     /locus_tag="VGB68_10590"
                     /inference="COORDINATES: protein motif:HMM:NF022359.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2695 domain-containing protein"
                     /protein_id="HEY0049725.1"
                     /translation="MPIVRKREIENLEQMNGEEIEAFLANLPSRKDQIEDLFDFIEFK
                     LEREPCNHSLRFAMQYMMENRLNFPKVTSWLNENGGYCDCKVLE"
     gene            complement(270..1544)
                     /locus_tag="VGB68_10595"
     CDS             complement(270..1544)
                     /locus_tag="VGB68_10595"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0049726.1"
                     /translation="MLLDITPLRVSRDYRLLFFGQLISFFGSMMTFIIVPWQMLKLTN
                     SNEMVGYIYLAEFVPMVSLAFIGGALADALDKRKMLRLTEIGQTFVTLILLLNSLLPA
                     PRLWVLYVAVALHAGFAALQRPAFESLIQKIIPDELMTAVAALNSLRYNFGAIISPSI
                     GGLIAAYFTAGVAYAIDLVTFAASLVAVFLISAVPPPENAERPGLRSIIEGCRYALRR
                     QELLGTYLIDICAMFFAFPQALYPALAAMYGEAYLGFFPAALSLGMLTASFTSGWTKN
                     IHRHGLMVTIAAALWGVGIIFFGLSESIWVALFFLAAAGFFDMISGVFRMTIWNQTIP
                     NYLRGRLASLEMISYLTGPMLGGAKMGIVAERFSVKTAIISGGILCVVTVALTAIFLP
                     KFIRYDGREGIKQREIEEAERAELLRVSEGEF"
     gene            complement(1681..1935)
                     /locus_tag="VGB68_10600"
     CDS             complement(1681..1935)
                     /locus_tag="VGB68_10600"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEY0049727.1"
                     /translation="MENTDLKTRTKSFALRIIKLYKALPNTVEAQIIGKQILRSGTSA
                     GAQYREGTRARSNAEFISKIESALQELEETAYWLELLIEA"
     gene            complement(2009..>2243)
                     /locus_tag="VGB68_10605"
     CDS             complement(2009..>2243)
                     /locus_tag="VGB68_10605"
                     /inference="COORDINATES: protein motif:HMM:NF023380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3471 domain-containing protein"
                     /protein_id="HEY0049728.1"
                     /translation="KISFAEENGRLVVRLSHSPTYIGDLRHWENDKFRVVWRDPVAEK
                     TFLAFAVKNGKVESVKMAMTGFIDDAEYEYFKR"
BASE COUNT          580 a          591 c          522 g          550 t
ORIGIN      
        1 tcctccaaaa ctttgcagtc gcagtagccg ccgttttcgt tgagccagct cgtgactttc
       61 ggaaaattca ggcgattttc catcatatac tgcatcgcaa agcgcaggct gtgattgcac
      121 ggttcgcgtt cgagcttgaa ttcgataaaa tcgaataaat cttcgatttg gtctttgcgc
      181 gacggcaaat tcgcgagaaa cgcttcgatt tcttcgccgt tcatttgctc taaattttcg
      241 atttctcttt ttctgacaat cggcattttt taaaattctc cttctgaaac tcttaataat
      301 tcggctcttt cggcttcttc gatttctctt tgtttgatgc cttcgcgccc gtcgtaccgg
      361 ataaatttcg gcagaaatat cgccgtcagc gcaaccgtca caacgcataa gattccgccg
      421 gaaatgattg ccgttttgac gctgaagcgt tcggcgacga ttcccatttt cgcgccgccc
      481 agcatcggtc ccgtcaggta gcttatcatt tcgaggctgg cgagacgccc gcgcaggtag
      541 ttcggaatcg tctgattcca gatggtcatg cggaaaactc ccgaaatcat atcgaaaaag
      601 cccgctgccg ccagaaaaaa cagcgcgacc cagatgcttt ccgacaatcc gaaaaagata
      661 atcccgacgc cccacaaagc ggcggcaatc gtcaccatca aaccgtgccg gtgaatattt
      721 ttcgtccagc ccgaggtaaa actcgccgtc agcataccga gcgagagcgc cgccggaaaa
      781 aatccgagat aagcctcgcc gtacatcgcc gccagcgccg gatagagcgc ctgcggaaaa
      841 gcaaaaaaca tcgcgcagat atcaatcaga taagttccca aaagctcctg acgccggagc
      901 gcgtagcggc agccttcgat aatacttcgc aagccgggac gctcggcgtt ctccggcggc
      961 gggacggcgc ttatcaaaaa aactgcgacc agcgaggcgg caaaagtaac taaatcaatc
     1021 gcgtaagcga cgccggcggt aaaatacgcc gcaatcaagc cgcctatcga cgggctgata
     1081 atcgctccga aattatatcg aagcgagttt aaggcggcga ccgccgtcat caactcgtcg
     1141 ggaataattt tttgaatcag cgattcgaaa gccggacgct gcagcgcggc aaaaccggcg
     1201 tgaagcgcga ccgccacgta taaaacccag agccgcggag cgggcaaaag cgaattgagc
     1261 agcagaatca gcgtcacgaa agtctgcccg atttccgtca ggcgcagcat ttttcgttta
     1321 tccagcgcgt ccgccagcgc gccgccgata aacgccagcg aaaccatcgg gacaaattcc
     1381 gccagataaa tataaccgac catttcgttg gaattagtca gtttcagcat ctgccacgga
     1441 acgatgataa aggtcatcat cgagccgaaa aacgaaatca actgtccgaa aaataaaagg
     1501 cgataatcgc gcgagactct gagcggcgtt atatctaaaa gcatatttaa aatgatgaac
     1561 gcgaaacgat gaacgatgaa ttcatttttt catcttagcg gtttttacgg atgagacgaa
     1621 aatcgcggtc aattcttcag tttcccgata aagctctttt agtttatttt cgcttatgat
     1681 ttacgcttca atcaagagtt ccagccaata cgcggtttcc tcaagctcct gcaaggcgct
     1741 ttcaatctta ctgataaact ccgcattaga cctggcgcgc gtgccttcgc gatattgcgc
     1801 gccggctgaa gtgccgcttc gcaatatttg tttaccgata atttgcgcct cgactgtgtt
     1861 aggcaaggct ttgtaaagtt tgataattcg caaagcaaat gatttggttc tggtttttaa
     1921 atcagtgttt tccatttcat agatagaatt ggcgtttcag aaaataccgc agctttgcat
     1981 aaacaattca tcattcatcg ttccgcgttt atcgtttaaa gtattcgtat tccgcgtcgt
     2041 cgataaaacc cgtcattgcc atttttaccg attcgacttt cccgtttttg accgcaaagg
     2101 cgagaaaagt tttctccgcc accgggtcgc gccagacgac gcgaaactta tcgttttccc
     2161 agtgccgcaa atcgccgatg taggtcggcg aatgcgaaag gcggacgacg agcctgccgt
     2221 tttcttcggc gaacgatatt ttg
//
LOCUS       DASWNE010000535         4233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_329955, whole genome shotgun sequence.
ACCESSION   DASWNE010000535 DASWNE010000000
VERSION     DASWNE010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329955"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1218
                     /locus_tag="VGB68_10610"
     CDS             <1..1218
                     /locus_tag="VGB68_10610"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEY0049729.1"
                     /translation="HSAGSRAKSVRLALVYSIGIILTFTALGMLLAIFVGAAGINLFA
                     ANPWVNLLITGIFLFFAFNLFGSYEINVPTSILTRLDNLTRSQEGEGSGFVGALLMGL
                     TFTLTSFTCTSPFVGTILVSASQGSWQMPLVGMLAFSTVFALPFFVLALVPQWVSQLP
                     RAGGWMNSVKVSMGFLEIAAAMKFLSNVDLVWHWGIFTRPVVLAIWIAIGIILAIYLL
                     GKFQLSLDSKPERIGAFRLISAIVSLAISFYLLTGLFGARLGELESFLPPETETSRSS
                     FNNSKSPAEPKWTNNDFEGALKQAKTENKMVFVDFTGYTCTNCRWMEANMFPKREVEA
                     ELNKFVLARLYTDGNGEIYEKQQQFQEQTFQTVALPFYAIFDADGKSIATFPGLTRNA
                     QEFVDFLQKSQKN"
     gene            1363..1938
                     /locus_tag="VGB68_10615"
     CDS             1363..1938
                     /locus_tag="VGB68_10615"
                     /inference="COORDINATES: protein motif:HMM:NF017778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HEY0049730.1"
                     /translation="MKTVKIENLIEGLREIPDAEFTCDDVYRFLAENPVDVDTIAPYF
                     FWCERFYTRNLIYKDARFELMTLCWERGQVSRVHNHADQRCWMTVPVGKLRGQNFRAV
                     ELDEETGFCKLEKTDRFDLSDCLAAKVELEEPIHQILNLPEFDERAVSLHIYSKPFDK
                     CLSYCLETDRFAEVPLSYTSIKGELCDGIKL"
     gene            1955..2305
                     /locus_tag="VGB68_10620"
     CDS             1955..2305
                     /locus_tag="VGB68_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049731.1"
                     /translation="MKKFFPLIIFALALCAATACRKAQAPAPNENLNANSGSNTAGNV
                     AAADAAEKEREEIQKESERVKNDLLELQKKIEEAQGDSRQLEQRVRQTRKAIDATQKK
                     IDENQKKLNEALPK"
     gene            2321..2740
                     /locus_tag="VGB68_10625"
     CDS             2321..2740
                     /locus_tag="VGB68_10625"
                     /inference="COORDINATES: protein motif:HMM:NF019401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEY0049732.1"
                     /translation="MFLQELARNLAGKSTFESVEQPVVGAVQWLRLGVETIGAAIICF
                     GVILAVYRLIQHFRDEQSRDFNYIRLTLGKYLALALEFQVGADILSTAIAPGWEQIGK
                     LAAIAAIRTLLNYFLTRELENERKREEQTPTRAETNV"
     gene            2741..>4233
                     /locus_tag="VGB68_10630"
     CDS             2741..>4233
                     /locus_tag="VGB68_10630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY0049733.1"
                     /translation="MNQNEKKWLIVTGLICLVYASLRFWNLTASCLWFDEIFSLHAAA
                     HDWQNLFRFVAQDLIHPPLFYVLLKIWTAIVGDALFPLRFFPVFFSILALAPFILLCR
                     ELKLSRAAVALALIFFAVNGSLIKYAQEIRMYSLLMFFALVSYWLFARFLNSGKGFLS
                     LLIVNILLIYTHYFGWFVVFSEVIAVAVLSREKLKPVLLLSGATFLGFTPWIYALWEA
                     SKINADVGQNIGWMTKPKLRAILQFVYDLFEPIYFQQSNADAAAILFLTLPIILLGII
                     AFVFRLANLKNESRADKQNLILLSIFIATPLLSAFVASWISPYSIWGTRHLIIVFAPF
                     AILLAVGFSKINIFKLQMPALGLIFLLFAGAFVLQARQPKPDFIWCAWENLALDLRSE
                     KEAKIYVFEDLVAYHFWFALRDADANFEVVKIDGIEGLTEDKAYFLPRGFDKVKTAVE
                     SDVTGERFFIAFRDKDWNEAKPPLKNLIEKGYRIGAPKVLEAQGLKA"
BASE COUNT          971 a         1039 c         1097 g         1126 t
ORIGIN      
        1 cattcggcgg gcagccgcgc caaatccgtc cggctcgcgc tggtttattc aatcggaatt
       61 atcctgactt ttaccgcgct cggaatgctt ctggcgattt tcgtcggcgc ggcgggcatc
      121 aatcttttcg cggcaaatcc gtgggtcaat cttttaatca ccggaatttt tctttttttc
      181 gcgtttaatc ttttcggctc gtatgaaatc aacgtgccga cttccatttt gacgaggctg
      241 gacaacctga cgcgcagtca ggaaggcgag ggcagcggtt ttgtcggcgc gcttttgatg
      301 ggcttgacgt ttacgctcac ttctttcacc tgcacgtcgc cgtttgtcgg cacgattctg
      361 gtttcggcgt cgcagggcag ctggcagatg ccgctcgtcg ggatgctcgc gttttcgacc
      421 gttttcgctt tgccgttttt cgttctcgcg ctcgttccgc aatgggtttc gcaattgccg
      481 cgcgcgggcg gctggatgaa ttcggttaaa gtttcgatgg gctttctgga aatcgcggcg
      541 gcgatgaagt ttctttcaaa cgtcgatttg gtctggcact ggggaatttt tacgcgcccc
      601 gtcgtgctgg caatctggat tgccatcggc ataattctgg cgatttatct tttaggaaaa
      661 ttccagcttt cgctcgattc aaaaccggaa agaatcggcg cgtttcgcct gatttcggca
      721 atcgtttcgc tggcgattag cttctattta ttgaccggac ttttcggcgc gaggctcggc
      781 gagctcgaat cgtttctgcc gccggagacc gaaacctcgc gcagcagctt taacaactcg
      841 aaatcgcccg ccgaacccaa gtggacaaac aacgatttcg aaggcgctct gaaacaggcg
      901 aaaaccgaaa ataaaatggt tttcgtcgat tttacgggtt acacctgcac gaactgccgc
      961 tggatggaag cgaatatgtt tccgaaaagg gaagtcgaag cggaattgaa taaatttgtg
     1021 ctggcgcgcc tttacaccga cggcaacggc gagatttacg aaaaacagca gcagtttcag
     1081 gagcagactt ttcaaaccgt cgcgctgcct ttttacgcca tcttcgacgc cgacggcaaa
     1141 tcaatcgcga catttcccgg tctgaccaga aacgcgcagg aatttgttga ctttttgcaa
     1201 aaatcgcaga aaaattaacc acagagacat agaaaaagat tcaaacagga tagacaggat
     1261 gaacaggata attaaaaatg cgaacgttga tttctgattt ttgtcctgtc tatcctgtcc
     1321 atcctgttaa aatttccctt tgtgtccttg tgtcgaaaaa gtatgaaaac ggtcaaaatc
     1381 gaaaatttaa tcgaaggtct gcgcgagatt cccgacgcgg aatttacctg cgacgacgtc
     1441 taccggtttc tcgcggaaaa tccggttgac gtcgacacaa tcgcgccgta ttttttctgg
     1501 tgcgagagat tttacacgcg gaacctgatt tataaggacg cgcggtttga gctgatgact
     1561 ttgtgctggg agcgcgggca ggtctcgcgc gtgcataatc acgccgacca gcgctgctgg
     1621 atgacggttc ccgtcggcaa attgcgcggg cagaattttc gcgccgtcga attggacgag
     1681 gaaacgggct tttgcaaatt agagaaaacc gaccgtttcg acctttccga ctgcctcgcc
     1741 gccaaagtcg aattggaaga gccgattcac cagattttga atctgcccga attcgacgag
     1801 cgcgccgtca gcctgcacat ctattccaag cctttcgata aatgtctttc gtattgtctg
     1861 gaaaccgacc gctttgccga agttccgctt tcatacacaa gcattaaggg cgagctttgc
     1921 gacggaatca aattgtaaac gccggagaat tgaaatgaaa aaattctttc cgctcatcat
     1981 attcgcgctc gctctgtgcg cggcgactgc ttgccgaaag gctcaagcgc ccgcgccgaa
     2041 tgagaatctt aatgctaatt ccggctcgaa caccgccgga aacgtcgcgg ctgccgacgc
     2101 cgccgaaaaa gagcgcgaag aaatacagaa agaatccgag cgcgtgaaaa acgaccttct
     2161 cgaactccag aaaaaaatcg aggaagcgca gggcgacagc cgccaattgg agcaaagagt
     2221 tcgccagacc cgaaaggcga ttgacgcgac gcaaaagaaa atcgacgaaa atcagaaaaa
     2281 gctgaacgaa gcgcttccga aatgaagccg gagttttaaa atgtttcttc aggagctcgc
     2341 ccgaaattta gccgggaaaa gcacgtttga aagcgtcgag cagccggtcg tcggggctgt
     2401 tcaatggctg cgtctgggcg tcgagacgat cggcgcggcg attatttgtt tcggcgtaat
     2461 tctggcggtt tacaggctta tccagcattt cagagatgag cagtcgcggg atttcaatta
     2521 tatacgtctg acccttggca aatatctggc gctggcgctg gagtttcagg tcggcgccga
     2581 tattttgtcg acggcgatag cgccgggatg ggaacaaatc ggcaagctcg ccgccatcgc
     2641 cgccatccgc actttgctga attattttct gaccagagag ctggaaaacg agcgcaagcg
     2701 cgaggaacaa acgccgacaa gggctgaaac gaatgtctaa atgaatcaaa acgaaaaaaa
     2761 atggttaatc gtcacgggct tgatttgtct tgtttacgcg tcgctgcgat tctggaattt
     2821 aacggcttcg tgtctctggt ttgacgagat tttcagtctt cacgccgccg cgcacgactg
     2881 gcagaatctt tttcgctttg tcgcgcagga tttgattcac ccgccgcttt tttacgtttt
     2941 attaaaaatc tggacggcta tcgtcggcga tgcgcttttt ccgctcagat tttttccggt
     3001 tttcttttcg attctcgccc ttgctccatt tattttactt tgccgcgaat tgaagctttc
     3061 gcgcgccgcc gtcgcgctgg ctttaatctt tttcgccgtc aacggctcgc tcatcaaata
     3121 cgcgcaggaa atcagaatgt attcgctttt gatgtttttc gcgctcgttt catactggct
     3181 tttcgcgcgc tttttaaatt cggggaaagg ttttttatct ttattaatcg tcaatatttt
     3241 actgatttac acgcactatt tcggctggtt cgtcgtcttc tcggaggtga ttgccgtcgc
     3301 cgttttgagc cgcgaaaaat taaagccggt tctgctgctc tccggcgcaa cgtttctcgg
     3361 cttcacgccc tggatttatg ctttgtggga agcctcgaaa ataaacgccg acgtcgggca
     3421 gaatatcggc tggatgacaa agccgaagct gcgggcgatt ttgcagttcg tctatgattt
     3481 gttcgagccg atttattttc agcagagcaa cgccgacgcc gcggcgattt tgtttctgac
     3541 tttgccgatt atcctgctcg gcattatcgc gtttgttttc cggctggcaa atttgaaaaa
     3601 tgaaagccgc gccgacaaac aaaacctgat tttgctgtcg atttttatcg cgacgccgct
     3661 tttatcggct tttgtcgcca gttggatttc gccttattcg atttggggca cgcggcacct
     3721 gattatcgtt ttcgcgccgt ttgcgattct gctggcggtc ggcttttcca aaataaacat
     3781 ttttaaatta caaatgcccg cgctcggctt gatttttctt cttttcgcgg gcgcgttcgt
     3841 tttgcaggca agacagccga agcctgattt catatggtgc gcgtgggaaa atctggcgct
     3901 tgatttgcgc tcggaaaaag aggcgaaaat ttacgttttt gaagatttgg tcgcttatca
     3961 tttctggttc gccctgcgcg atgccgacgc gaatttcgaa gtcgtaaaaa tcgacggaat
     4021 cgagggctta accgaagaca aagcgtattt tctgccgcgc ggtttcgaca aagtgaaaac
     4081 cgccgtcgaa agcgacgtga cgggcgaaag attcttcatc gcctttcgcg acaaggattg
     4141 gaacgaagcg aaaccgccgc tgaaaaatct gattgaaaaa ggctatcgaa tcggcgcgcc
     4201 gaaagttttg gaagcgcagg gtttgaaagc gtt
//
LOCUS       DASWNE010000536         5327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_583302, whole genome shotgun sequence.
ACCESSION   DASWNE010000536 DASWNE010000000
VERSION     DASWNE010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583302"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1349
                     /locus_tag="VGB68_10635"
     CDS             <1..1349
                     /locus_tag="VGB68_10635"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEY0049734.1"
                     /translation="AISGLPVPAKDAQIPFYKSVLADIGDNQSLSANLSTFTSHISNI
                     AGMMRECRNPALVLLDEVGTGTDPEEGSALGVAIVDHFRRRCSAQVIASTHYRGLKIY
                     ATNDETVINASVEFDEKTLQPTYRLLTGLAGASSGIEIARRFGIPDDVIEKAKDNLDV
                     SASEVADYLMKIRRESKIAEDLRVALEEEREATAKKYALLDIEARKKEKERQKEFENE
                     LSETISGFERQAKAFVSGIEDKALKARLEKEMQARKAELKRAAMTKVGDGLTRGQAGA
                     ENNSPFAVRQPQLSDRPIEAGTRVLLKTFGSVGTVEKIDGETAEVLVGSMRLREKISN
                     LQAVAAEEKKTESKLEKLQKQSSDALLRLDTENMNVELNIIGKTTAEVDDLVDPFLDD
                     AYLNGFQRVRIIHGIGTGALKSAVHRHLKGHQHVSRYTLAPREQGGEGATVVELKQ"
     gene            complement(1725..3713)
                     /locus_tag="VGB68_10640"
     CDS             complement(1725..3713)
                     /locus_tag="VGB68_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049735.1"
                     /translation="MKRFESKLFSIIAFFLLTTAAAFGANTFEGYSLIVSADDTGACP
                     VQYRPGGSNDIDVFIAGTTTPATGLTACGGSAVSGNNVTPNGDGQWCFTGANDYYDIK
                     LDTGLIYLWHPKTKSTGFHNVKDFRPVVGGVQTEPVDYTRTIKNAVAFIASRNGGTLY
                     FPEGKYIVGTTDGNTRDANYTPITLPSGIVIVGASPQYSIPTTNVPAKQSATTIRLRN
                     NNQTIFRIGGCTDQIIIRNLELLGNSATFGEQPRDSTGNTGIEGLGKWAINPTTQAET
                     PNSSQVFKFENVTFQNLDKGIWVHNANDGNCNASTQQCHSWQFDYVKVDHGTFINNKT
                     GIWVNTYNTDWKITNSVFLYSAQNAPGNGIRLEKAGSALIEQSFGGGYDYAGAIGGTF
                     VYVDTIGSLSIINSAAERGQRSIYVNPLGAISNLMLTVINSIFGDKIELGGRMQYVST
                     GNFYGAKTVTSTADVKITSTGDRFCYDPAVLPGRCKDENGVTVSNPGFNGGKVMFQTG
                     RPTEGAGADQIQRRPNFFGYDVEVADDDYNNNEPMIYSHSYNYNKPLLRLGQPGFHYD
                     FKRREFNGFLTITGNQDKPYRGVTINGPLQFDKDMTYNDIVLYGNSWLSGYPVITDGV
                     LVYCKDCAKNTTTGLCQQGTAGTDGAFAKRINGTWRCD"
     gene            4067..4195
                     /locus_tag="VGB68_10645"
     CDS             4067..4195
                     /locus_tag="VGB68_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049736.1"
                     /translation="MGFIFLINAFSLAPRASIKLETKWKKTSGKAALEYTSSRSLK"
     gene            complement(4583..>5327)
                     /locus_tag="VGB68_10650"
     CDS             complement(4583..>5327)
                     /locus_tag="VGB68_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049737.1"
                     /translation="IYVNPAGSISTTMMVVVGSHFGDKVELNGRMNYISSGTFYGAKT
                     VIAGPNVKITSTGDRFCYDPAITPGFCRDENNQPVPNPGFQGGKIMFQTGRLAEGSGN
                     NFIQRRPNFFGHDVEIRDDDETNEEPLLSAKSLSFNKPLLRLGQTNFWYDFKRDESTG
                     FLSIRGNQDKPYVGFTINGLIQMDKNTTFNDLIVYATTISNGQPLITDGAIVYCKDCA
                     KNASGVCIQGTAGTDGAFAKRINNAWKCD"
BASE COUNT         1267 a         1370 c         1397 g         1293 t
ORIGIN      
        1 tggctatttc cggcttgccc gtgcctgcca aagacgcgca gattccgttt tataaatccg
       61 tcctggcgga catcggcgac aatcaatctc tgagcgcgaa tctttcgacg tttacgtcgc
      121 atatttcaaa catcgccgga atgatgcgcg aatgccgcaa tccggcgctc gttttgctgg
      181 acgaagtcgg cacgggcaca gacccggaag aaggctcggc tctgggcgtg gcgattgtcg
      241 accattttcg ccgccggtgt tcggcgcagg tcatcgcctc gacgcattat cgagggctta
      301 aaatctacgc gacgaacgac gaaaccgtga taaacgcttc ggtcgaattc gacgagaaaa
      361 ccttgcagcc gacttatcgt cttttaacgg gtttggcggg cgcgtcatcc ggaatcgaaa
      421 tcgcgcggcg cttcggaatt ccggacgacg ttatcgaaaa ggcaaaagac aatcttgatg
      481 tttcagccag cgaagtcgcc gattatctga tgaaaatccg tcgcgaatcg aagattgccg
      541 aggatttgcg cgtcgcgctg gaagaagagc gcgaggcgac cgcgaaaaaa tacgcgctgc
      601 tcgacatcga agcgcggaaa aaggaaaagg aaaggcagaa ggaatttgaa aacgagctga
      661 gcgaaacgat ttccggcttt gaacgacagg cgaaagcctt cgtctccggc atcgaggaca
      721 aagctttaaa agcgcgtctg gaaaaggaaa tgcaggcgcg caaagccgag ctgaaacgcg
      781 cggcaatgac aaaagtcggc gacggattga cgcgcgggca ggcgggcgcg gaaaacaatt
      841 cgccgttcgc cgttcgccag ccgcaattga gcgaccgccc catcgaagcc ggaacgcgcg
      901 ttcttctgaa aactttcggc tccgtcggaa cggtcgaaaa aatcgacggc gaaacggcgg
      961 aagttctggt cggctcgatg cgcctgcgcg agaagatatc gaatctgcaa gccgtcgcgg
     1021 cggaagaaaa gaaaacggaa agcaagctcg aaaaattgca aaaacagtcg agcgacgcgc
     1081 tcttgaggct cgacacggaa aatatgaacg tcgagctgaa catcatcggc aaaacgacgg
     1141 cggaagtcga cgatttggtt gacccgtttc tggacgacgc ttatctgaac ggattccagc
     1201 gcgtgcgaat cattcacggc atcggcacgg gcgcgctgaa atcggcggtt catcgccatc
     1261 tgaaaggaca tcagcacgtt tcgcgctaca cgctcgcccc gcgcgagcag ggcggcgaag
     1321 gcgcaaccgt ggtcgagctg aaacaataat tcaggattca ggattcagga ttcaggattt
     1381 aagattcaag atttaagatt caagatttaa gattcaagat ttaagattca agattcaaaa
     1441 tagaagaaca gatttaatcc tgaatcctga atcctgaatc ttgaatcctg aatcctgaat
     1501 cctgaatctt gaatcctgaa tcctgaatcc tgaatcctga atcctgaatc ttcgacgttt
     1561 tgcattaagc aaatacgcgg aaacgcgaca gtgagggagc gtgtcttgtc ggctgaagaa
     1621 acgaggggtt ctaccgatgc tgccctcgcc tctttttccg gttttccatc taacccgaca
     1681 cgccccgtca ctatcgcgtt tccgcttttg attaaccctg aagcttaatc acatcgccag
     1741 gtgccgttga tgcgcttggc aaacgcgccg tccgtgcccg ccgttccctg ctggcaaagc
     1801 ccggtcgtcg tgttcttcgc gcagtcttta cagtagacca gcacgccgtc ggtaattacc
     1861 gggtaaccgc tcaaccagga gttgccgtaa agaactatgt cgttgtaggt catatcctta
     1921 tcgaactgca acggaccgtt gatggtgacg cccctgtagg gcttgtcctg attgccggtg
     1981 atggtcagaa aaccgttaaa ctcgcgacgt ttaaagtcgt agtgaaagcc gggctgcccg
     2041 aggcgcagca ggggtttgtt gtaattatac gagtgtgaat aaatcatcgg ctcgttgttg
     2101 ttgtagtcgt cgtcggcgac ttccacgtca taaccgaaaa agttcgggcg tctttgaatc
     2161 tggtcagcgc ccgcgccttc ggtcgggcgt ccggtctgaa acattacctt tccgccgtta
     2221 aagccgggat tgctgacggt aaccccgttt tcatccttgc agcgaccggg cagaaccgcc
     2281 gggtcgtagc aaaaacggtc gccggtcgag gtgattttca cgtccgccgt ggatgtcacg
     2341 gttttcgcgc cgtagaaatt tccggtcgaa acatactgca tacgaccgcc gagctcgatt
     2401 ttatcgccga aaatgctgtt gataaccgtc agcatcaggt tggagatcgc gcccagcggg
     2461 ttgacgtaaa tgctccgctg cccgcgctcc gcggcggagt tgataatcga aagcgagccg
     2521 atcgtatcga cgtaaacaaa cgtgccgccg atggcgccgg cgtaatcgta tccgccgccg
     2581 aagctttgct cgatcagtgc cgagcccgct ttttcaagcc gaatcccgtt gcccggcgcg
     2641 ttctgcgcgc tataaagaaa caccgagttg gtgattttcc aatcggtatt gtaagtgttg
     2701 acccagatgc cggttttatt gttgataaag gttccgtggt cgaccttcac gtaatcaaac
     2761 tgccacgaat gacactgctg ggtcgaggca ttacaatttc cgtcgttggc attgtgaacc
     2821 cagatgcctt tgtccagatt ttgaaaagtg acgttttcaa acttgaaaac ctggctcgaa
     2881 ttgggcgttt cagcctgcgt cgtcgggttt atcgcccatt tgcccaagcc ttcgattccc
     2941 gtattgccgg tcgaatctcg cggctgctcg ccgaaggtcg ccgagttgcc gagcagttcc
     3001 agattgcgaa taataatctg gtcggtgcag ccgccgatac gaaaaatcgt ttgattgttg
     3061 ttgcgcaggc gaatcgtcgt cgcgctttgc ttggcgggaa cattggttgt cgggatggaa
     3121 tactgcgggc tcgccccgac gatgacgatg ccggacggca gcgttatcgg cgtgtaattc
     3181 gcgtctctgg tattgccgtc ggtcgttccg acaatgtatt tgccttccgg gaaataaagc
     3241 gtcccgccgt tgcgcgaagc gataaaggcg acggcgtttt tgattgtcct ggtgtaatca
     3301 accggttctg tctggacgcc gccgactacg gggcgaaaat ccttgacgtt atgaaacccg
     3361 gtactcttcg ttttcggatg ccataaatat atcaagcccg tatcgagctt aatgtcgtaa
     3421 taatcgttgg cgcccgtaaa acaccactgc ccgtcgccgt tcggcgtcac gttattgccg
     3481 ctgaccgcgc tgccgccgca agccgtcaga ccggtcgcgg gcgtcgtcgt tcccgcgata
     3541 aaaacgtcta tgtcgttgct gccgccggga cgatactgca ccgggcaagc tcccgtatcg
     3601 tccgccgaga cgataagcga atagccttca aaggtgttcg cgccgaaagc cgcagccgtc
     3661 gtcaacaaaa aaaacgcaat aatagaaaat agtttcgatt caaatctttt cattttcaaa
     3721 ctctccttaa ctttctccgc ctgaggcgcg gagagacaaa tgccttgacc gggcgaatct
     3781 tcaaaacgca aacttcggaa aatttgcctt caaagcttcg acagtcgttt tgaaatcggt
     3841 tcgttttctc aatttatcgt cgagaaaaca cgctccgact ccgccttttt gttaggaaag
     3901 gatcaaaaga aataacgatt gaagtcaggg attgactttt cgcttgcgaa tgcagtagcg
     3961 gcgcgatatg atacggttac catccgggta tcattatgtt acgcttgctc cggcatcgta
     4021 tctgatgacc ctgataaaac ccgcctgatt ggttacagcc gatcgggtgg gctttatttt
     4081 tttaatcaat gctttcagcc tcgctccgcg cgcttcgata aagctcgaaa cgaaatggaa
     4141 aaagacttcc ggcaaggcgg ctttggaata cacttccagc cgctcgttga aataaaattt
     4201 agcttgggaa caaaaccgat ttcggcactg ttagcggtcg ggagaaccgt gctgagtcga
     4261 accggtaaat ttttgtaaat cattgtatga tttacactta attcgctggc aaaaggaaac
     4321 gaatcaaatt aatttggaca tcttcaaact tactctcttt gccgctggaa gtcaatcttt
     4381 ttctctcttt ttttatgaaa aatccggtcg cttttccggc gctgcgttaa cccttgaaag
     4441 actacggttt aggataatat tcgttttgat ttgatccgaa aaaaaacgaa aatgcggcgc
     4501 ttcgcatttc accgagccgg cgcattttca ttttttttta actaaagcct gaattacaaa
     4561 aatcaaagaa gcgcgccgac gctcaatcgc atttccaggc gttattaatg cgcttggcga
     4621 atgcgccgtc cgtgcccgcc gttccctgta tgcatacgcc cgaagcgttt ttcgcgcagt
     4681 ctttgcaata gacgatcgcg ccgtcggtaa tcagcggctg accgttggaa atggtggtgg
     4741 cgtaaacaat caggtcgttg aaagtcgtat ttttgtccat ttgtatcaaa ccgttaatcg
     4801 taaaaccgac atacggcttg tcctgattgc ctctgatgct cagaaaaccc gtgctttcgt
     4861 cgcgcttaaa gtcataccag aaattagtct gtcccagccg cagcagaggt ttgttgaaac
     4921 ttaatgattt ggcggatagc agcggctctt cgttcgtctc gtcgtcgtcc ctgatttcga
     4981 cgtcgtgccc gaaaaagttc ggtcgtcgct gaataaaatt attcccgctt ccttctgcca
     5041 agcgcccggt ctggaacatt atttttccgc cttgaaaacc gggattcggg accggttgat
     5101 tgttctcgtc gcgacagaaa ccgggcgtga ttgccgggtc gtagcagaaa cggtcgccgg
     5161 tcgaagtgat tttcacattt ggaccggcga tgacggtttt cgcgccgtag aaagttccgc
     5221 tcgagatgta attcattcga ccgttcaatt cgactttatc gccgaaatga ctgccgacaa
     5281 cgaccatcat cgtcgtcgaa attgagcccg ccggattgac gtagatg
//
LOCUS       DASWNE010000537         2126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_330026, whole genome shotgun sequence.
ACCESSION   DASWNE010000537 DASWNE010000000
VERSION     DASWNE010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330026"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..1077)
                     /locus_tag="VGB68_10655"
     CDS             complement(181..1077)
                     /locus_tag="VGB68_10655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEY0049738.1"
                     /translation="MDSEKIKKFFVRLYKKINDDDILGNAAQVAFYFTFALFPLLLFL
                     ISLFGIILNSTDDLRGELFVYLRQIMPGSAFTLIETTLQEVAENTSGSKLTIGILITL
                     WSASAGIDNLRGALNEVYNLKENRSWFKTKLLAVLLTLGIGVLIFVAIVSVFYGTHLL
                     DYLLPIDSPYLLMPLRWLIVLVALIMTFALLYNFAPNHDAFDWKWITPGALVGIVLWL
                     LLSGIFRVYLNYFDSYAKTYGSLGAVIILLLWLYLTALVILIGGAINAIFDEESGVEK
                     ENEDPKQVEEEETQTGKENRAN"
     gene            1169..>2126
                     /locus_tag="VGB68_10660"
     CDS             1169..>2126
                     /locus_tag="VGB68_10660"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HEY0049739.1"
                     /translation="MAAEAPNPSANRLVSLDVFRGMTVAGMVLVNNPGTWSAIYDPLE
                     HAQWNGITPTDYVFPFFLFIVGVAISLALGKRVEAGGAGAEIYRKIITRAIVIFLLGL
                     FLTTFPFYNFTKGEWLDVSNVRIMGVLQRIAVCYLCAALIFVQTNWRQQAIIAAALLL
                     LYWALMTLIPVPGCEVTSVYDKACNLAAYLDRAILTENHIWKQGKVYDPEGLLSTIPA
                     IATTLAGVLCGHWIRRKIDDHEKVSAMFFFGVALLALGYAWQLAFPLNKSLWTSSYVV
                     YTAGLALCFLGFCYWLIDIKGYRAWARAFVIFGVNALALFVGS"
BASE COUNT          534 a          515 c          535 g          542 t
ORIGIN      
        1 cggctttcgc gcgctcggct tgaatggttt tttccagtat cgcgggctgt tccgcaatct
       61 cctgcaacat taaagacata ggttttaact gatagttgat agttgataac tgaaaactaa
      121 aactgatagt tgaaaattaa aaaagctatc agttatcagt tatcagttat caactatcag
      181 ttaattagcg cgattttcct ttccggtctg cgtttcttct tcttcgacct gtttcgggtc
      241 ttcgttttct ttttcgacgc cggattcctc gtcaaaaatg gcgtttatcg cgccgccgat
      301 aaggataacc aaagccgtca ggtaaagcca caacaaaaga ataataaccg cgccgagcga
      361 gccgtaggtt ttcgcgtagg aatcgaaata gttcaggtaa acgcggaata tgccggaaag
      421 caaaagccac aggactatgc cgaccagcgc gccgggcgtt atccatttcc agtcaaacgc
      481 gtcgtggttg ggcgcaaaat tataaagcag cgcgaaggtc ataataagcg cgaccagaac
      541 gattaaccac ctgagcggca tcaaaagata cggcgaatcg atcggcaaaa gataatcgag
      601 caggtgcgtg ccgtaaaaaa ccgagacgat tgccacaaaa atcaaaacgc cgatgccgag
      661 cgttaaaagc accgccagaa gcttcgtttt aaaccacgag cggttttctt tcaggttgta
      721 aacttcgttg agcgcgccgc gcagattgtc gattccggcg gaagccgacc agagcgtaat
      781 caaaattccg atcgtcagtt tactgcccga agtattttcg gcgacttcct gcagcgtcgt
      841 ctcgattaaa gtaaatgccg acccgggcat tatctgccgc agataaacaa aaagttcgcc
      901 gcgcaaatcg tcggtgctgt tcaggataat accgaaaaga cttatcaaaa acagtaaaag
      961 cggaaacagc gcgaacgtaa aataaaaggc gacctgcgcc gcattgccga gaatgtcgtc
     1021 gtcatttatt tttttataaa gacgcacgaa aaacttctta attttctccg aatccataaa
     1081 accctccccg aatcgtttga cacaatttaa ttgtagagta aatataacta aaactaagtc
     1141 gttaagacaa caaaagaagg aaaaatttat ggcagcagaa gcgccgaatc cgtcggcaaa
     1201 ccgcctcgtc tcgctcgacg tttttcgcgg gatgaccgtc gcgggaatgg ttctggtcaa
     1261 taatccggga acgtggtcgg cgatttacga cccgctcgaa cacgcccagt ggaacggcat
     1321 cacgccgacc gattacgttt tcccgttttt tctgtttatc gtcggcgtgg cgatttcgct
     1381 ggctttggga aaacgcgtcg aagccggcgg cgcgggcgcg gaaatttatc gaaaaatcat
     1441 cacgcgcgcg attgtgattt tcctgctcgg tttatttctg acgacgtttc ctttctacaa
     1501 ttttacgaaa ggcgagtggc tcgacgtttc aaacgtgcga ataatgggcg ttttgcagcg
     1561 catcgccgtt tgctacctgt gcgcggcttt gattttcgtg cagacgaact ggaggcagca
     1621 ggcgattatc gccgccgctc tgctattgct ttactgggct ttgatgactt tgattcccgt
     1681 tcccggctgt gaagtgacga gcgtttacga caaagcctgc aatcttgccg cctatctcga
     1741 ccgcgcgatt ttgacggaaa atcacatctg gaaacaagga aaagtttacg acccggaagg
     1801 cttgctgagc acgattccgg cgattgcgac gacgctggcg ggcgttttgt gcgggcattg
     1861 gattcgacgg aaaatcgacg accacgaaaa ggtttcggcg atgttttttt tcggcgtcgc
     1921 gctgctggct ctcggctacg cctggcaact ggcttttccg ctcaacaaat ctctctggac
     1981 aagctcgtat gtcgtttata cggcaggttt ggcgttatgt tttctcggct tttgctactg
     2041 gctgattgac atcaagggct accgggcgtg ggcgcgagcc ttcgttattt tcggcgtcaa
     2101 cgcgttggcg ttatttgtcg gttcgg
//
LOCUS       DASWNE010000538         5699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_123247, whole genome shotgun sequence.
ACCESSION   DASWNE010000538 DASWNE010000000
VERSION     DASWNE010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123247"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..877)
                     /locus_tag="VGB68_10665"
     CDS             complement(167..877)
                     /locus_tag="VGB68_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049740.1"
                     /translation="MRSKFVPAIVSLICLGSLVVLGSSTTVIGNLLESVNGNSFTKVF
                     SSASQSAPAQSFENNPALVLPEKPAPVKEVPQTVLWHMIFSLSRMTEQEAKKLNQQGR
                     NGDAWSQYFIKRGVLSAASEKTFKQTAAAYLKDLEPIDRRAKEVTDAMRAEYPKGLIK
                     DPKKMPLPPAELGALQQQKNALVLRHRDAFKNAVSPESFNKFSDFLTKDFSKSVAPVN
                     YPHPNQQIGKRKEVGDEK"
     gene            1238..2401
                     /locus_tag="VGB68_10670"
     CDS             1238..2401
                     /locus_tag="VGB68_10670"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0049741.1"
                     /translation="MDFTTKELLKRYAKLAIGQPFAPILLNILVTSVCDMRCTHCFFT
                     DELDDRPRKKLQMKTHELEKISETLGGNLGVLVIAGGEPFTRKDLPEIVGAFHRNNKL
                     ESVYLMSNGQIHPRIFPDVTRICQENPNLNVTVALGIDGLKDQHEKIRQKAGSWDKAI
                     HTARTLKEMKKEFPNIDVQTCTCVMNSNQDTIFEWYDFLKYDLKPDKININYIRPPSK
                     DPNELNFDHQRYAKLSNMILEDVRNSAIKNNYGGSAGFFKAAIDIYMHDLIAKVKETN
                     EAQMTCYAGTAGAVIYDEGTISSCENKSEVTNLRDWDWNFQAAWNSPQMKQRRKEVAA
                     GCFCTHESNSYYPSLPFNPKHLIQIKKLEKQMKKASRELENNQPQTEGVAIKA"
     gene            2465..2761
                     /locus_tag="VGB68_10675"
     CDS             2465..2761
                     /locus_tag="VGB68_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049742.1"
                     /translation="MKKIIQLTALFLVIGSLLIFETASSEAKSGESDSAVSNDNQNRS
                     ANRSKRKGNSKGNRNSGINGNSNRNSNFNGNSNRGDDDDGGHRRGRKRRGRGRD"
     gene            2789..3982
                     /locus_tag="VGB68_10680"
     CDS             2789..3982
                     /locus_tag="VGB68_10680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0049743.1"
                     /translation="MQQNAPKILIISSDTGGGHRSAAQALVEGVNRFWYGGSKAVRVI
                     RAVEESHHITEKLVNLYNWVLRNRQHWMKYVFWAMNRIRPETREFVHKRCIGYVREQF
                     EKWCPHIVVSVHPLTQHIFARFLKELKLSEQVPLVTVVTDPCYGFWKGWACNDVSLYL
                     VANEDAQRQLIDYGIAPEKIKISGMPVHPKFHEVSEKDAQEARRVFGLDPEKFTVFVN
                     AGWVGGGNIPQIFRELVRGDLDVQAVFLAGKNEELKKEAEEIAKHAKFPVKVIGYSDD
                     IEKLMQSANVMISKLGGLTTFEALACHLPIIADTTTPPMPQEAGTVNILAKRGAGILL
                     EKASDIVPTIQSLLNDSKKYAEMRAAAVGLAMPNSTQRIIEEIAALLPAHLETEPTQA
                     LSAAN"
     gene            4218..4967
                     /locus_tag="VGB68_10685"
     CDS             4218..4967
                     /locus_tag="VGB68_10685"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0049744.1"
                     /translation="MSLLLEGKRAFVSGGTRGIGAAICEVFAREGADVAFNYNSRDDL
                     AEQVKQKIESYGRRALSFKVSVTDRFGMKHIAREIKDVWGSINVLVNNAAVNKPDNFA
                     TTTDKSWDWVVDTNVNSLFAVTKPFYKQMIRERGGSILNITSVGAIRSLPTSVHYATS
                     KAAMIGFTKCLSREAANFGITVNAIAAGIFDTDLGNTLPERLLQMHDFWVSKGRLGKP
                     QELAEFAAFMVSDRNSFMNGEIVIVDGGAVT"
     gene            complement(5063..5587)
                     /locus_tag="VGB68_10690"
     CDS             complement(5063..5587)
                     /locus_tag="VGB68_10690"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0049745.1"
                     /translation="MKPDVKIRRAAVKDANLLAALGGATNYETYFETDEPEDLAKYIA
                     DFFNPPAMKIELEDRNNSFFIAEINSKAVGYAKLREGQPAECVKGENTVELQRIYVLE
                     KMTRHGIGKILLQTCLDEAKSRGFDALWLAVFELNTRAFEFYKRRGFEQVGESEFYYG
                     EQRFNCFVMKIACR"
BASE COUNT         1505 a         1367 c         1441 g         1386 t
ORIGIN      
        1 gtaataagcg cccgcgtaat aatcgaccgc cgttatcgat aagccgtcga tgttcggcgc
       61 gtcgtcgtcc caggcaatga ccgaagcgcc ccagaccgac tgagctttcg tttccacgtc
      121 gaaagtgaca acggcaaaca aaagaaagaa aaagattaaa atatatttac ttttcatcgc
      181 cgacctcctt gcgtttgccg atttgctggt tcgggtgcgg atagttgacc ggcgcgacgg
      241 atttcgagaa atctttcgtc agaaaatcgg agaacttgtt aaacgattcc gggctgacgg
      301 cgtttttaaa cgcgtcgcgg tggcgcagca cgagcgcgtt tttctgctgc tgcaaagcgc
      361 ctaattccgc cggcggcaaa ggcatctttt tcgggtcttt gatgagtcct ttcggatatt
      421 cggcgcgcat cgcgtcggtg acttccttgg cgcggcggtc aatcggttcg aggtctttca
      481 gataagcggc ggcggtttgc ttgaaagttt tctcgctcgc ggcggaaagc acgccgcgtt
      541 tgataaagta ttgactccac gcgtcgccgt ttctgccctg ctgattcaat tttttggctt
      601 cctgctcagt catgcgcgaa agactgaaaa tcatatgcca taaaaccgtc tgcgggactt
      661 ccttcacggg agccggtttt tcgggtaata cgagcgccgg attgttttca aacgattgag
      721 ccggtgcgga ttggcttgca gatgagaaga ctttagtaaa cgaattgccg ttgacggatt
      781 cgagtaaatt gccgatgacc gtagttgagc tgccgagaac gacaaggctg cccagacaaa
      841 tcagcgaaac aattgccgga acgaatttac ttcgcattag agatactcct taaattgtga
      901 ttttagatat ggggcaacta tcgggatggt ttgggagaag ctgagaatga gaattcctga
      961 cttcaggacg cgagcaaatt gagttccgcc cgagccgaat ttgataattt ccccattcta
     1021 tttttgagac gcggtagctg tatcgtgtcc tgtaggtcaa atcaaggctg gctttataaa
     1081 attttacaat tatgttgtaa gcaaaaatga tgcaattaaa aataaagggc ttatgcgcgc
     1141 ctttcttgct ctcgaccacg aatgtaagat aaattaaaac gtgagggcaa cttaacgcat
     1201 caaaacgcgg gacaaaattc tcacaaggaa taaaaaaatg gattttacga cgaaagaatt
     1261 attaaaaaga tatgcgaagc tggcaatcgg acagccgttt gcgccgattt tattaaatat
     1321 tttagtgacg agcgtctgcg atatgcgctg cacgcactgc ttttttacgg atgaactgga
     1381 tgaccgaccg cgcaaaaaat tgcagatgaa aacgcacgag ctggaaaaaa tttccgaaac
     1441 tctgggcggc aatctcggcg tgctggttat cgccggcggc gagcctttta cccgcaagga
     1501 tttgccggaa atcgtcggcg cgtttcatcg aaataataaa ctcgaatcgg tttatctgat
     1561 gtcgaacggg cagattcacc cgcgaatttt tcccgacgtg acgcgcatct gccaggaaaa
     1621 tccgaatctg aacgtgaccg tcgcgctcgg catcgacggt ttgaaagacc agcacgaaaa
     1681 aattcgtcag aaagcgggaa gctgggacaa agcgattcac acggcgcgca ctctgaagga
     1741 aatgaaaaaa gaatttccga acatcgacgt gcagacctgc acctgcgtga tgaattcgaa
     1801 tcaggacacg attttcgaat ggtacgattt tctgaaatac gatttgaagc ccgacaaaat
     1861 caatatcaac tatattcgcc cgccgtcgaa agacccgaat gagcttaatt tcgaccacca
     1921 gcgttatgcc aaattgtcga atatgattct ggaggacgtg cgcaattcgg caatcaaaaa
     1981 caattacggc ggctcggctg ggtttttcaa agcggcgatt gatatttata tgcacgacct
     2041 gattgccaag gtcaaggaaa ccaacgaagc gcagatgacg tgctacgccg gaactgccgg
     2101 agcggttatt tatgatgaag gaacgatttc gtcgtgcgaa aacaaatcgg aagtgacgaa
     2161 tctgcgcgac tgggattgga attttcaagc cgcgtggaac tcgccgcaga tgaaacagcg
     2221 ccgcaaggaa gttgccgcgg gctgcttctg cacgcacgaa tcgaacagtt attatccgag
     2281 cttgccgttt aatccgaagc acctgattca gattaaaaag ctcgaaaaac agatgaaaaa
     2341 agcgtcgagg gaactggaaa ataaccagcc gcaaaccgaa ggcgtcgcta tcaaagcgta
     2401 aattgataat tttttcgtgt cttttctcct tgtttcgccg aactgtgaaa caaggagaaa
     2461 atttatgaaa aaaatcattc agttgacggc tttatttttg gtaatcggca gtttattaat
     2521 ctttgaaacc gcgtcgagcg aagcgaaatc cggcgaaagc gattcggcgg tttcaaatga
     2581 caatcagaac cgaagcgcga atcgctcgaa acggaaaggg aattcgaagg gaaatcgaaa
     2641 ctccggcatt aacggaaact cgaaccgcaa ttccaatttt aacggcaact cgaatcgcgg
     2701 cgatgacgac gacggcgggc accgccgagg acgaaagcgg cgcggcagag ggagagatta
     2761 acttccttct gcttgcaact tatataaaat gcagcagaac gctcccaaaa ttctcattat
     2821 ttcatcagac acgggcggcg gacatcgttc cgccgcgcag gctttggtcg aaggcgtcaa
     2881 tcgtttctgg tatggcggct cgaaagccgt ccgggtcatc cgcgccgtcg aagaatctca
     2941 tcacatcacg gaaaaacttg taaatcttta caattgggtt ttgcgaaacc gccagcactg
     3001 gatgaaatac gttttctggg cgatgaatcg cattcgaccg gaaacgcgcg aatttgttca
     3061 taagcgctgt atcggttacg tccgcgagca gtttgaaaaa tggtgcccgc atattgtggt
     3121 cagcgtgcat cctttgacgc agcatatctt cgcgcgattt ttaaaggagc tgaaactttc
     3181 cgaacaggtg cctctcgtca cggtcgtcac agacccgtgt tacggcttct ggaaaggctg
     3241 ggcgtgcaac gacgtttcgc tctatctggt cgccaacgaa gatgcgcaga gacaattgat
     3301 tgattacggc atcgcgccgg aaaaaatcaa gatttcggga atgcccgtgc atccgaaatt
     3361 tcacgaagtt agtgaaaaag acgcgcagga agcgcgccgc gttttcggct tagacccgga
     3421 gaaattcacg gtttttgtca atgccggatg ggtcggcggc ggaaacattc cgcagatttt
     3481 tcgcgagctg gtgcgcggcg atttggacgt gcaggcggtt tttctcgccg gaaaaaacga
     3541 ggaattgaaa aaagaagccg aagaaattgc caaacacgcc aagtttccgg tgaaagttat
     3601 cggttattcg gacgatatcg aaaaactgat gcagtcagcc aacgtgatga tttcaaagct
     3661 cggcggcttg acgacctttg aagcgctcgc ctgtcacctg ccgattatcg ccgacacgac
     3721 cacgccgccg atgccgcagg aagccggaac ggtcaatatc ctggcaaaac ggggcgcggg
     3781 aatactgctg gaaaaagcgt ccgatattgt tccgaccatt caaagcctgc tcaacgattc
     3841 caaaaaatac gcggaaatgc gcgccgccgc ggtcggcttg gcaatgccga attcgacgca
     3901 gcgaatcatc gaagaaatcg cggcgctgct gcccgctcat ctggaaaccg agccaactca
     3961 agctctcagc gcagcaaact aaaatttttc aatgcggaca aagaaaatac agtgagaaca
     4021 gcattgacgg cgaacaaggg aaattgattt taatccacgc taattctgtt atcaacggtt
     4081 aatttttttt caaaaatctc cgcctcttcg cctctgtgcg gcaaaatttt ctgtttgtct
     4141 ctacttgtca catctgcgca ttttcattaa ccttttgttt tagagctttt acgaaattct
     4201 tatttggaga aattattttg agtcttttac tcgaaggaaa acgcgctttt gtttccggcg
     4261 gcacgcgcgg aatcggcgcg gcgatttgcg aagttttcgc ccgcgaaggc gcggacgtcg
     4321 cttttaacta caattcacgc gacgatttag ccgagcaggt caagcagaaa atcgaatctt
     4381 acggcaggcg cgcgctcagc tttaaagttt ccgtgaccga ccgcttcggc atgaagcata
     4441 tcgcgcgcga aatcaaagac gtgtggggtt cgataaacgt tctggtcaac aacgccgccg
     4501 tcaacaagcc ggataatttt gcgacgacga ccgacaaatc gtgggattgg gtcgtcgata
     4561 ccaacgtcaa cagccttttc gccgtcacca agccttttta taaacagatg atacgcgagc
     4621 gcggcggctc gattctaaat atcacgtcgg tcggcgcgat tcgctccctt ccgacttcag
     4681 tgcattacgc aacttccaaa gcggcgatga ttggctttac gaaatgtctc tcgcgcgaag
     4741 ccgccaactt cggaatcacc gtcaacgcga ttgccgccgg aattttcgat acggacctgg
     4801 gcaacactct gcccgaacgc cttttgcaga tgcacgattt ctgggtatcg aaaggtcggc
     4861 tcggcaagcc gcaggagttg gctgaatttg cggcgtttat ggtcagcgac cggaacagct
     4921 ttatgaacgg cgaaatcgtc atcgtggacg gcggcgcggt gacttaattt cggcaagtgt
     4981 cggtagcctg acgctgagga agggcttcag tccgtagcgt aaagacttca aatcgtaatg
     5041 aaagcccgcg ctcatgctta ggctaccgac acgctatctt catcacaaag cagttaaaac
     5101 gctgctcgcc ataataaaat tcgctctcgc cgacctgttc gaagcctcgt cttttataaa
     5161 attcaaaagc gcgcgtgttc agttcaaaaa ctgcgagcca gagcgcgtca aaacctctcg
     5221 attttgcttc gtccaaacac gtttgcagca aaattttgcc gattccgtgc cgcgtcattt
     5281 tctccagaac gtaaatccgc tgcagttcga ccgtgttttc gcccttcacg cattcggcgg
     5341 gctgaccttc gcgcagcttg gcatagccga cggctttgct gtttatttcc gcgataaaaa
     5401 atgaattgtt ccggtcttcc agctcgattt tcattgccgg cggattaaaa aaatcggcga
     5461 tgtattttgc caaatcttcc ggctcgtcgg tttcaaaata ggtttcgtaa ttcgtcgcgc
     5521 cgcccagagc agccagtaga ttggcatctt tgacagccgc gcggcgaatt ttcacatctg
     5581 gtttcattgt ttaatgctct attttaatgt ggtttgccga taaacagata gtcgagaaac
     5641 gatgcagccg actgtaaatt ctaaattatt ttgaaaaaca ttttgcgggt taatttata
//
LOCUS       DASWNE010000539         4281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_370252, whole genome shotgun sequence.
ACCESSION   DASWNE010000539 DASWNE010000000
VERSION     DASWNE010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370252"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /locus_tag="VGB68_10695"
     CDS             complement(<1..1016)
                     /locus_tag="VGB68_10695"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0049746.1"
                     /translation="MSQAKETTKRQKLWVVTELYYPEMTSTGYYLTRIAEGLAKDFEV
                     KVLCGQPNYSARGVLAPSHEIYHEVEIFRCVGTRLDKNVIPFRLINMLTLGASVFFKA
                     LLNFKKGDRILVVTTPPSLPFITALAALSRGAAYTLLIHDNYPEILIAVEKTKPDSPL
                     VKTLSFFNRWLYKYASKIIVVGRDMKELVEEKTSGLDIPIATIPNWAELETVEPKPRA
                     ENKLLQELNLSDKFVFLYAGNMGYPNDLESIVWCADKLKTDERFHFVFLGAGVKRKWL
                     EREAEEKGFRNVTILDSRPRSEQNTFLNGCDVGIVSLVKKMWGVSMPSRTYNILAVGK
                     PILA"
     gene            complement(1017..2117)
                     /gene="wecB"
                     /locus_tag="VGB68_10700"
     CDS             complement(1017..2117)
                     /gene="wecB"
                     /locus_tag="VGB68_10700"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEY0049747.1"
                     /translation="MKVLTIFGTRPEIIRLSLIMKILDQNCEHVTVHTGQNYHESLSD
                     VFLRELEVRTPDFHFGIKANSFGEQIGQILTKTDEILAETKPDRVLILGDTNSALTAI
                     VAARRGIPVFHMEAGNRCYDNRVPEEVNRRIVDHSSALLLPYTERSKENLVREGIERE
                     RIYITGNPINEVLQTYSEKIDASDALNQFGVKEYEYFLVTLHRAENVDLMERLQKILQ
                     GLSRTVEKFDKPMLVSVHPRTAEKLAQFGLQPESEKIKLLNPLGFFDFVKLEKNALAV
                     LTDSGTVQEECAIFGIPNVTLRDVTERPETIEAGSNILSGAETESILRAVELALSQPA
                     NWIAPKEYLAENVSQTVSKIVLGYTSLRKHVS"
     gene            complement(2127..3170)
                     /locus_tag="VGB68_10705"
     CDS             complement(2127..3170)
                     /locus_tag="VGB68_10705"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEY0049748.1"
                     /translation="MNLEGKRILVTGGTGSLGQTLVRRLLTGEMGMPARITVFSRDEA
                     KQHYMRLEYLHKRNATDDIIYQDAPDLLTFRIGDVRDYSSLLAAMREADIVFNAAALK
                     QVPSCEYFPFEAVLTNIYGAENVARAVRENNLPVERVIGISTDKACKPINVMGMTKAL
                     QERILIEANRDCPRTSFSCVRYGNVIASRGSIVPLFVEQVRKNQPITVTLPEMTRFLL
                     SLDRAVDTVFACLKDGKRGETYIPQVPAARITDLAKALMGEKDLPIIYTGIRPGEKIH
                     EIMVSEEEIYRTVERNGYYVIMPVLPELREKEEFTPALTSEYSSKDDNISVEDLKVLL
                     GGANSEIASFMNA"
     gene            complement(3183..4076)
                     /locus_tag="VGB68_10710"
     CDS             complement(3183..4076)
                     /locus_tag="VGB68_10710"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0049749.1"
                     /translation="MRILIFGGTGMLGHKLVRQWRDEFKVFSTVRGDFSKYKRAGLFE
                     KDEIISPVEVEDIESVRNAVKISQPEVIVNCVGIIKQLPTSKNVIKTLSVNSIFPHRL
                     AEIAQENNARLITISTDCVFNGKKGNYGEADVSDAEDVYGKSKNLGEILSENCLTIRT
                     SIIGRELETAHSLVEWFLSNRGGKVRGFTKAVYSGFPTIILAEIIAGLIVKHKNLSGL
                     YHVSSEPIDKYRLLCMIRDAYQQDVEIEPSDEFRIDRSLDSSKFRQATGFIPESWEEM
                     IKKMARDSFPYEEVRKQLITG"
BASE COUNT         1038 a         1119 c          918 g         1206 t
ORIGIN      
        1 aaagccagaa tcggcttgcc gacggctaaa atattgtatg tcctagacgg catcgaaacg
       61 ccccacattt ttttgaccag cgagactatt cctacgtcgc agccgtttaa aaatgtattt
      121 tgttcgctgc gcgggcgcga atctaaaatc gtcacgtttc gaaatccctt ttcttccgct
      181 tcgcgttcca gccattttct tttcacgccc gcgccgagaa aaacaaaatg aaatctttcg
      241 tccgttttca atttatcggc gcaccagacg attgattcca gatcgttcgg gtagcccata
      301 tttcccgcat acaggaaaac gaatttatcc gataaattca attcctgcaa aagcttgttt
      361 tcggcgcgcg gcttcggctc gaccgtctcc agctccgccc agttcggaat cgtcgcaatc
      421 ggaatatcga gccccgaagt tttttcttcg accagctctt tcatatcgcg cccgaccact
      481 atgattttgc tcgcgtattt gtaaagccaa cggttaaaaa agcttagcgt tttaaccagc
      541 ggtgaatcgg gtttggtttt ttccaccgca atcagaattt ccggataatt atcgtgaatc
      601 agcagagtgt aagccgcgcc gcgcgacaac gccgccagag ccgttataaa aggcaggctc
      661 ggcggcgtcg tgacgaccag aatgcggtcg ccttttttaa aattcagcag cgctttgaaa
      721 aaaaccgaag cgccgagcgt cagcatattt ataagccgaa acggaatgac gtttttgtcc
      781 agcctcgttc ccacacaacg aaaaatctcg acttcgtggt aaatttcgtg actcggagcc
      841 aaaacgccgc gcgccgaata attcggctgc ccgcataaaa ccttaacctc gaaatctttc
      901 gccagacctt cggcaatccg agtcaggtaa tagccggtcg aagtcatctc cgggtaataa
      961 agctctgtga cgacccagag tttttgtcgt tttgttgtct ctttcgcttg gctcacctat
     1021 gaaacgtgtt ttctcaggct cgtgtagccc aaaacgattt tgctgaccgt ctgcgaaaca
     1081 ttctccgcca gatattcttt cggcgcaatc cagtttgccg gctgcgaaag cgcgagctcc
     1141 accgctctca agatgctttc ggtttccgcg ccgctcagga tattgcttcc cgcttcgatt
     1201 gtttccggtc tttcggtcac gtcgcgcagg gtgacgttcg gaattccgaa aatcgcgcat
     1261 tcttcctgca ccgttccgct gtcggtcaaa acagccagag catttttttc cagtttgacg
     1321 aaatcgaaaa agcctaacgg attgagcagc tttatttttt ccgattccgg ctgtagaccg
     1381 aattgcgcga gcttttccgc cgtgcgcggg tgtacgctga cgagcatcgg tttatcgaat
     1441 ttttcgaccg tccgcgacag cccctgcaga attttttgca gtctttccat caagtccaca
     1501 ttttccgctc gatgcagagt gaccagaaaa tattcatact ctttgacgcc gaattgattc
     1561 agagcgtcgc tggcatcgat tttctcggag tatgtctgca aaacttcatt tatcggattt
     1621 ccggtaatat aaattcgctc tcgctcgatg ccctcgcgaa ccagattttc tttgctgcgc
     1681 tcggtataag gcagcaaaag ggcgctcgaa tgatcgacaa tacgccggtt gacttcttcc
     1741 gggacgcgat tgtcgtaaca acggtttcca gcctccatat gaaaaaccgg aattccgcgc
     1801 cgcgccgcta caatggcggt cagagcgctg ttcgtatcgc ctaaaatcaa aacgcggtcg
     1861 ggtttggttt ccgccagaat ctcgtcggtt ttcgttaaaa tctgcccgat ttgctcgccg
     1921 aaactgttcg ccttgatgcc gaaatgaaaa tccggcgtcc ggacttccag ctcgcgcaaa
     1981 aatacgtcgc tcaagctttc gtgataattc tgccctgtgt gaacggtcac gtgctcgcaa
     2041 ttctggtcga gaattttcat aatcagactc agacggatta tctccggtct ggttccgaaa
     2101 atcgttaata ctttcataaa taaattttaa gcgttcataa aggaggcgat ttccgagttt
     2161 gcgccgccga gcaaaacttt caggtcttcg acggagatat tgtcgtcttt ggaagaatac
     2221 tcgctcgtca gcgcgggcgt aaactcttct ttttctcgaa gttcgggcaa aaccggcatt
     2281 atcacgtaat agccgtttcg ctcgacggtg cgataaattt cttcttcgga aaccataatt
     2341 tcgtgaattt tttcgcccgg tcgaatgccg gtgtaaataa tcggcaaatc cttttcgccc
     2401 atcaaagcct tcgccaggtc tgtaattctc gccgccggaa cttgcggaat ataagtttcg
     2461 ccgcgttttc cgtctttcag acaggcgaaa accgtatcga cggctctatc cagactcaaa
     2521 agaaaacgcg tcatttccgg cagagtaaca gttatcggct gatttttgcg aacctgctcg
     2581 acgaacagcg gaactatcga gccgcgcgag gcgatgacgt tgccgtaacg cacgcaggaa
     2641 aacgatgtgc gcgggcaatc gcggttagcc tcaattaaaa ttcgttcctg caaggctttc
     2701 gtcattccca taacattaat aggcttgcag gctttatccg tcgaaattcc gattactctc
     2761 tcgaccggga ggttattttc gcgaaccgcc cgcgcgacat tctccgctcc gtaaatattg
     2821 gttaaaaccg cttcgaacgg aaaatattca cacgacggaa cctgttttaa agcggctgcg
     2881 ttaaagacaa tgtccgcctc gcgcattgcc gccagcagcg acgaataatc ccgcacgtcg
     2941 ccgatgcgaa aagtcagcaa atccggagca tcctgataaa taatatcgtc cgtcgcgttc
     3001 cgtttatgca gatattcgag ccgcatataa tgctgtttgg cttcgtcgcg agaaaagacg
     3061 gtgatgcgcg ccggcattcc catctcgccc gtcagcaaac gcctgaccag tgtttgaccg
     3121 agcgagcccg tgccgcccgt tactaaaatt ctttttcctt ctaaattcat ttaatattgc
     3181 ttttatccag taattaattg ttttctgact tcctcgtacg gaaacgaatc gcgagccatt
     3241 ttttttatca tttcttccca gctttcggga ataaatccgg tcgcttgcct gaatttcgat
     3301 gagtccagac tgcggtcaat cctgaactcg tcggaaggct cgatttccac gtcctgttga
     3361 taagcgtccc gaatcataca aagcagtcga tatttatcaa tcggctcgct cgaaacgtga
     3421 taaagcccgc tcaaattttt gtgtttgaca atcaaaccgg caattatttc cgctaaaatt
     3481 atggttggaa aaccggaata gacagccttt gtaaatcctc tgacttttcc gccgcgattg
     3541 ctcaaaaacc attctaccag actatgcgcg gtttccagtt cgcgcccgat gatggaagtg
     3601 cgaatcgtca gacaattttc cgaaaggatt tctcctaagt ttttgctctt gccgtagaca
     3661 tcttcggcat cggacacatc cgcctcgccg taatttcctt ttttgccgtt aaatacgcag
     3721 tccgtgctga tggttattaa acgcgcatta ttttcctgcg ctatctccgc cagccgatgc
     3781 ggaaaaatag aattcacgct cagtgttttt ataacatttt tcgaagtcgg caattgcttg
     3841 ataataccga cgcagttaac aatcacttca ggctgagaaa ttttcaccgc attgcgcacg
     3901 gattcaatat cttcaacctc gaccggagaa attatttcgt ctttttcgaa taacccggct
     3961 cttttatatt ttgaaaaatc gcctctgacg gtcgagaaaa ctttaaattc atctcgccat
     4021 tgccgaacca gcttgtgacc gagcattccc gtgccgccaa atattaaaat tctcattata
     4081 attgccttaa agttttatat taactgtctg cctgccgacg cttaataata tttttaaatc
     4141 aataaaccga ataatatgaa taattatctt tatttctttc gagttcccat taaactgtaa
     4201 ttcaaaacat cttcggtagc tttttcaatt tccgtgtctg acagccaatg gttcaagtaa
     4261 tttctagcat ttgttgccaa a
//
LOCUS       DASWNE010000540         5553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_195389, whole genome shotgun sequence.
ACCESSION   DASWNE010000540 DASWNE010000000
VERSION     DASWNE010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5553
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195389"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..315)
                     /locus_tag="VGB68_10715"
     CDS             complement(34..315)
                     /locus_tag="VGB68_10715"
                     /inference="COORDINATES: protein motif:HMM:NF024843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxC-x17-CxxC domain-containing protein"
                     /protein_id="HEY0049750.1"
                     /translation="MPDVEIPCVQCKEIFLFTEKEQETFYRQNLMQPQRCQKCRSKKA
                     AGGGNASTRFEIICDHCGKHDHVPFQPKVGRSVLCKECHQASKARMRSA"
     gene            680..880
                     /locus_tag="VGB68_10720"
     CDS             680..880
                     /locus_tag="VGB68_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049751.1"
                     /translation="MSIGLVAKLALVTTMAIASIWGDIIAPVDSGPCSDPGSYNCCVH
                     QCIHDSSTGCNGGTRCCQNVCA"
     gene            complement(1004..1954)
                     /locus_tag="VGB68_10725"
     CDS             complement(1004..1954)
                     /locus_tag="VGB68_10725"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEY0049752.1"
                     /translation="MKTAIVHHPIYQKHDTGTGHPETPRRYEVVIKALKGDETLWANL
                     NEIQAEPVSKGIIQAAHTPQHFKRIEGAFANGVEMLDADTAISMQSFDAAVYGAGGAC
                     RAVDAVMNGEAKNAFVAVRPPGHHATSENAMGFCLFNNIAIAARYAQNKYKEIEKVAI
                     IDWDVHHGNGTQGIFFDDPTVFFFSQHQYPWYPGTGSAGEIGFGRGRGYTLNVPVKAR
                     TTAKTQKEMFARALENISGNFKPDFVMISAGFDAHASDPLGQLLIEDEDFAEMTRMVK
                     NWANDACQGRIVSALEGGYNLQTLGETVRAHVEELSREER"
     gene            complement(1982..2482)
                     /locus_tag="VGB68_10730"
     CDS             complement(1982..2482)
                     /locus_tag="VGB68_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049753.1"
                     /translation="MENIEIESIGKLKPHPDVPDEWLISEPVPISFFNGKELQFTLNG
                     DLKSDKSFLSDANRTIKNFLAKTGNDKLNLSNQIYKYYRAIQDYHDSELFGAEPLKLN
                     NEREIWNYVHPYEIYICRNAEPDADMYLQIQCDCDWEEEHGLQLVFKNGSELTRVSHI
                     DYNPVE"
     gene            complement(2487..3638)
                     /locus_tag="VGB68_10735"
     CDS             complement(2487..3638)
                     /locus_tag="VGB68_10735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0049754.1"
                     /translation="MKKSEIVIIGGGVVGASVAYHLTRRGARDVLILERENALGRGST
                     GAATGGVRAQFETDINIKMSLYSLDFFNNWDFDCGYEPRGYLFFATDEKQFEYVRRNV
                     EKQKSLGVKEVEIVDTQTISEIVRGLNCSDICGGSFGARDGFINPLGVLEGFTKKAIE
                     NGARVETGTEVFSIETENGKVGAVETNKGRIECEKIVLCSGAWARALAQTAGIDLPVE
                     PQKRQIVWAKSAAGFQANLPMVIDLSSGFHFRPAKDSKNEILFAYPDRAEKPGFSTDF
                     DENFIEKVYERAKYRAPFLYDTEVVREKCRAGLYENTPDHHAVLGGCAVEGLYFANGF
                     SGHGVMHSPATGRALAEIILDGEASFLDVSCLRLERFAKGELLHETAFI"
     gene            complement(3898..4842)
                     /locus_tag="VGB68_10740"
     CDS             complement(3898..4842)
                     /locus_tag="VGB68_10740"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY0049755.1"
                     /translation="MKLFGLLFLFVLTSVVQAQKPQRFIAVTIDDLPVVSTRGDLRAR
                     QEITGKLLAHITRAKIPAVGFVNEGKLYTADNKRSEAQIDLLRQWLDAGLELGNHTFS
                     HRSLNAIELKNYQDDLLRGEIITKELLKAKGKPMRYFRHPFLHTGLSLEVKENLDRFL
                     EKHAYTIAPVTIDNADWIFARAYDNAFDKGDKNLMKEIGAAYIPYLESKMDYWERQSS
                     KLFGREVKQILLLHANFINSDYFDDIAKMLKKRGYKFITLEEALKDESYKLPDTFTKR
                     SGISWLHRWALAKGSENVLPGEPKTPGFVLKAAGVESE"
     gene            complement(4996..5412)
                     /locus_tag="VGB68_10745"
     CDS             complement(4996..5412)
                     /locus_tag="VGB68_10745"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0049756.1"
                     /translation="MNNLFHLAFPVKDLEESRRFYGEILGCEEGRSSEAWIDFNLFGH
                     QIVAHLAPDGAGVRLRNEVDADHVPVPHFGIVLPMEEFRSFAERLKSKEVEFLIEPKI
                     RFEGETGEQATMFFLDPSGNALEFKAFADFSQVFAR"
BASE COUNT         1308 a         1455 c         1300 g         1490 t
ORIGIN      
        1 cattcacgat tcacgattta cgattcacga tttttaagcg gagcgcattc gcgccttgct
       61 cgcctgatgg cattccttgc ataagacgct gcgcccgact ttcggctgaa acggcacgtg
      121 gtcgtgcttg ccgcaatggt cgcagatgat ttcaaaccgc gttgaagcgt ttccgccgcc
      181 cgccgctttt ttcgagcggc acttctgaca gcgctgcggc tgcatcagat tctggcgata
      241 aaaagtttcc tgttcttttt ccgtaaaaag aaaaatttct ttgcactgta cgcacgggat
      301 ttcgacatcc ggcatcgcta tttctcctta aattaaatta aacccgacgt gacagagagt
      361 ttatcataaa taattcttaa cttgaaaaga gatacggcgc tgattcgagc agatgaatca
      421 ggattgttta aaactttatc ttcgattctt tccgcctgaa tcgtttcctt taagaccgcg
      481 cgcaaagact gcgaataaaa ccgagccggc ggcgcgaggt cattttaatg caaagttatt
      541 gcttcagctt tattttctta tttttcacga attaagcgca aaaaaccctt gactcttttt
      601 ttatttttgt atataactaa gctcacgtcc cccgaatttt ggttctaaat ctaaatctaa
      661 aaaaaagtga ggtaaattta tgtctattgg tctggttgca aaattagcgt tggtgacgac
      721 gatggctatc gcatccatct ggggcgacat tatcgctccg gtggacagcg gtccgtgcag
      781 cgaccccggc tcgtataact gctgcgttca tcaatgcatt cacgattcca gtacgggctg
      841 caacggcgga actcgctgct gccaaaacgt atgcgcgtaa aaagtcgctc gatgcggctt
      901 cgcgcctgaa acgggtttct gcatcggaag cctgcgggaa attcggcttc ggatgcagaa
      961 acttatctta attaatctta ataaattcta cagcgccggc gctctatctc tcttctctgc
     1021 ttaattcctc gacgtgagcg cggacggttt cgcctaaagt ctgcaaatta taaccgcctt
     1081 cgagcgcgga aacgatgcga ccctgacagg catcattcgc ccagtttttg accattcgcg
     1141 tcatctcggc aaaatcttcg tcttcaatca aaagctgtcc gagcgggtcg gaggcgtgcg
     1201 cgtcgaatcc ggcggaaatc attacaaaat ccggcttgaa atttccggaa atattttcca
     1261 gcgcgcgcgc gaacatttcc ttctgcgttt tcgccgtcgt ccgggctttt accggaacgt
     1321 ttaaagtata gccgcgcccg cgcccgaagc cgatttcgcc cgcgcttccc gtgcccggat
     1381 accacggata ttgatgctgc gaaaaaaaga aaaccgtcgg gtcgtcaaaa aaaattccct
     1441 gcgttccgtt gccgtgatgc acgtcccaat cgatgatggc gaccttttcg atttccttat
     1501 atttattttg cgcgtaacgc gcggcgatgg cgatgttgtt gaaaagacaa aagcccatcg
     1561 cgttttcgct cgtcgcgtgg tgtccgggcg ggcggacggc gacaaaagcg tttttcgctt
     1621 ctccgttcat caccgcatcg accgcgcgac aagcgccgcc cgcgccgtaa accgcagcgt
     1681 caaacgactg catcgaaatc gccgtgtcgg cgtcgagcat ttccacgccg ttggcgaaag
     1741 cgccttcgat gcgcttgaaa tgctgcggcg tgtgcgccgc ttgaatgatg cctttggaaa
     1801 ccggctcggc ttgaatttcg ttgagattcg cccaaagcgt ttcgtcgcct ttcagagcct
     1861 tgatgacgac ttcataacgg cgaggcgttt cgggatgccc agtgccggtg tcgtgttttt
     1921 gataaatcgg atgatggacg atggctgttt tcatataacg tttcaggatt tcacttttag
     1981 attattctac cggattataa tcgatatgac ttactctggt tagctccgag ccgtttttga
     2041 aaaccagctg caacccgtgt tcctcttccc aatcgcaatc gcactgaatt tgcagataca
     2101 tatcggcgtc cggttcggca tttcggcaga tatatatttc atacggatga acataattcc
     2161 aaatctcgcg ttcgttattc aattttaacg gctccgcgcc gaaaagctcg gaatcgtgat
     2221 aatcctgaat cgccctgtaa tatttatata tctgattcga gaggttcaat ttatcgtttc
     2281 cggttttcgc caaaaaattt tttatcgtcc ggtttgcgtc ggacagaaaa cttttgtcgc
     2341 ttttcaaatc tccgtttaaa gtaaactgca attccttacc gttaaaaaat gatatgggaa
     2401 cgggttcgct gattaaccat tcgtccggaa cgtccgggtg cggttttaat tttccgatcg
     2461 attcgatttc aatattttcc ataatttcaa atgaaggctg tttcgtgcag cagctcgcct
     2521 tttgcaaatc gttccagccg caaacacgaa acgtctaaaa atgaggcttc gccgtcgaga
     2581 ataatttccg ccagagcgcg tcccgtcgcg ggcgaatgca tgacgccgtg cccgctgaag
     2641 ccattggcga aatacaagcc ttcgaccgcg cagccgccca gaacggcgtg atggtcgggc
     2701 gtgttttcgt aaagcccggc gcggcatttt tcgcggacga cttcggtgtc gtataaaaac
     2761 ggcgcgcggt atttcgcccg ctcgtaaacc ttttcgataa aattttcgtc gaaatccgtt
     2821 gaaaagcccg gcttttcggc gcggtcggga taagcgaaaa gaatttcgtt tttcgaatct
     2881 ttcgccggtc gaaaatgaaa accgctgctt aaatcaatca ccatcggcag attcgcttga
     2941 aagcccgcgg cgcttttcgc ccagacgatt tggcgttttt gcggctcgac gggcaaatcg
     3001 attccggcag tctgcgccag cgcccgcgcc cacgcgcccg aacacaaaac gattttttcg
     3061 cattcgattc tgcctttgtt tgtttcgacc gctccgactt tgccgttttc ggtctcgatt
     3121 gaaaaaactt ccgttccggt ttcgacacgc gcgccgtttt caatcgcttt ttttgtaaaa
     3181 ccttccagca cgccgagcgg attgataaaa ccgtcgcgag cgccgaagct gccgccgcaa
     3241 atatcgctgc aatttaagcc tctgacgatt tccgaaattg tctgcgtatc gacaatctcg
     3301 acttctttga cgccgagcga tttttgcttt tcgacgtttc gccggacata ttcaaattgc
     3361 ttttcatccg tcgcaaaaaa cagataaccg cgcggctcgt atccgcaatc gaaatcccaa
     3421 ttattgaaga aatccagcga atacagcgac attttaatat taatatcggt ttcaaactgc
     3481 gcgcggacgc cgccggtcgc cgcgccggtc gagcctctgc ccagcgcgtt ttcgcgctcc
     3541 agaatcaaaa cgtcccgcgc gccgcgccgc gtcaggtgat acgccacgct tgcgccgacg
     3601 acgccgccgc cgatgatgac aatttcggat tttttcataa gatttcagcc gccgcaggtc
     3661 acagagttca cagagaaaat ttaaaattaa ccacagattt tacacggatt aacgcagatt
     3721 ttaacgggat ttacaggata agcaggataa aagacgagat tcgacgctcg catttttaaa
     3781 tcatcctgta aatcctgtgt atcctgtctg aatctgcgtt tatctgcgtt catctgcgtt
     3841 catctgcggt taatttctct ctgtgttctc tgtgacttct gcggctaatt caataattta
     3901 ttcggattcg acgcccgccg ctttcaatac gaaaccgggc gttttcggct cgccgggcaa
     3961 aacgttttcc gagcctttcg ccagcgccca gcgatgaagc cacgaaatgc cggagcgttt
     4021 cgtaaaagtg tcgggcaatt tataagattc gtcctttaag gcttcttcga gagtgataaa
     4081 tttgtagccg cgttttttga gcattttcgc gatgtcgtca aaataatcgg aattgataaa
     4141 attggcgtga agcagcaaaa tttgtttgac ttcgcgcccg aaaagcttcg atgactggcg
     4201 ctcccagtaa tccattttgc tttccagata cggaatatac gccgcgccga tttccttcat
     4261 cagatttttg tcgcctttat cgaaagcgtt gtcataagcg cgggcgaaaa tccaatcggc
     4321 gttgtcaatc gtgacgggcg cgatggtgta ggcgtgtttt tctaaaaacc ggtcgagatt
     4381 ttctttaact tccaggctca aacccgtatg cagaaacggg tggcgaaaat aacgcatcgg
     4441 cttgcccttc gctttgagca attctttggt gataatttcg ccgcgcagaa ggtcgtcctg
     4501 ataatttttt aattcaatcg cattcaggct ccgatgcgaa aacgtgtgat tgccgagctc
     4561 cagaccggca tcgagccatt gccggagcaa atcaatctgc gcctcgctgc gtttattgtc
     4621 tgcggtgtaa agctttcctt cgttgacaaa accgacggcg ggaattttcg cccttgtaat
     4681 gtgcgcgagc agttttccgg ttatttcctg ccgcgcccgt aaatctccgc gcgtcgaaac
     4741 gacgggcaaa tcgtcaatcg tcacggcgat aaatctttgc ggcttctgcg cctgaacgac
     4801 cgaggttaaa acaaataaaa acaataagcc aaacagtttc ataacaatag ccggaaccga
     4861 ccgcgcgcga acgcgtaagt ttcgggtttc cgtacccgcg tttcaattaa agagaatcag
     4921 aagcctttaa atttgtgaaa ccataaactt gaaaggctcg aattaaaccg cccgcccgcg
     4981 gcaggcggct cggaattacc tggcgaaaac ctgcgaaaaa tccgcaaacg ccttgaactc
     5041 cagagcgttt ccgctcgggt ctagaaaaaa catcgtcgcc tgctcgcccg tctcgccctc
     5101 gaaacgaatt ttcggctcga tgagaaactc gacctctttc gatttcagtc tttcggcaaa
     5161 gcttctaaat tcttccatcg gcagcacgat gccgaaatgc ggcacgggaa cgtggtcggc
     5221 gtcgacttcg ttgcgaagcc ggacacccgc gccgtcgggc gcgagatgcg cgacgatttg
     5281 atgtccgaaa agattgaaat caatccacgc ctcggagctt ctgccttctt cgcagccgag
     5341 aatttcgccg tagaaacgcc gcgattcttc caaatcctta accggaaaag ccaaatgaaa
     5401 taaattgttc ataagaaatt ttaaccgtaa atttgcgcaa agacaaaaaa gcggaaaacg
     5461 gtagacggta agcggcgggt tcgttatttt gcagccgcag ctttccgctt cgtacttccc
     5521 gtttaccgct ttccgcttcc cctgatttcc tcc
//
LOCUS       DASWNE010000541         5264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_688589, whole genome shotgun sequence.
ACCESSION   DASWNE010000541 DASWNE010000000
VERSION     DASWNE010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5264
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688589"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VGB68_10750"
     CDS             <1..732
                     /locus_tag="VGB68_10750"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY0049757.1"
                     /translation="KNWQPFEPTRYEQVEAMLPFTEILFKPGSKYGYSNLGIVFLGQI
                     IERLTGDDYEVYIDKNILKPLEMYRSYFDATPYHLLRYRSHSYFIENGKLTEGRFDAD
                     SGITVSNSGLNSPLPDIIKYLDFLTGDTEKKEIYDGILKRSSLEEMFQPTIPAPLDAN
                     GNAGFTTDVGLIFFLDRRGDETYFGHGGDQNGFISYIEFNPKKRTACVLIFNTNIILP
                     AGAPLEKDTVGKLRKASRQLHESID"
     gene            complement(736..1233)
                     /locus_tag="VGB68_10755"
     CDS             complement(736..1233)
                     /locus_tag="VGB68_10755"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY0049758.1"
                     /translation="MTTKEVLLEQFTACYDENGWFVALKNAFKDVSAEQAKWKAENAD
                     NSIWEILSHLNFYNEAYLKRFKGIDYVYPTSDNNETFSSAENASEEAWRAETERFDSI
                     MIEWRNLLEAADESKFDEAVSAANQSLWSSIVAHINVHNAHHAGQIVLLRKLQGSWDA
                     KQGVS"
     gene            complement(1233..3266)
                     /gene="tkt"
                     /locus_tag="VGB68_10760"
     CDS             complement(1233..3266)
                     /gene="tkt"
                     /locus_tag="VGB68_10760"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY0049759.1"
                     /translation="MTTAAQPKTKTDLDQLCINTIRTLSLDAVQKANSGHPGLPLGMA
                     PAAYVLWTKFLRHNPKNPKWFNRDRFLLSAGHGSMLIYSLLHLTGYDLPLGELKNFRQ
                     LHSKTPGHPENILTPGVEITTGPLGQGFANGVGMGIAQAHLAAKFNQKGFPVIDHFIY
                     CICSDGDLMEGVSYESSSLAGHLKLANLIYLYDDNRITIDGSTDLAFTEDVTKRFEAI
                     GWHVLEVADGNDLKAIEKAIKDAQKIKDKPKLIRVKTVIGFGMPKQGTSKAHSDAPGE
                     EAVKETKRNLGWDENKHFYIPKEVSAHFREAVKTGAKLEKDWEALIKKYEKAFPELGK
                     TLGDTRKGELRDDWEKSLPKFDEAEAKATRAYSGEVINAIAETVPQLVGGSADLTPSN
                     NTFIKASADFQAGRYEARNIHFGIREHAMGSALNGMALYGSVIPYGGTFQTFSDYMRP
                     AIRLAALSNIQVVFVFTHDSIGLGEDGPTHQSVEHLAALRAIPNLALIRPCDAHEVRE
                     AWRAALKRVCAPTAFALSRQKVALIDRKKFGDARGLHKGAYVLAEAEDKNGKETAPKL
                     ILLATGSEVGLAMQARELLNSQNIPTRVVSMPCWEFFDQQPAKYKESVLPAGVKARLA
                     IEAGVSLGWAKYVGLEGDTLSVDKFGASAPAEDVFRDYGFTVENVVKKAKKLL"
     gene            complement(3276..3530)
                     /locus_tag="VGB68_10765"
     CDS             complement(3276..3530)
                     /locus_tag="VGB68_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049760.1"
                     /translation="MEKRKSKAKKRQRLAELSKFFNLRKIDKSRFTFIVYEDAEELTT
                     FWLSTEKASPADFLYFEDKDYKVIKGEEFEPKKIDELFRK"
     gene            complement(3791..>5264)
                     /locus_tag="VGB68_10770"
     CDS             complement(3791..>5264)
                     /locus_tag="VGB68_10770"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEY0049761.1"
                     /translation="HVLAIDIGTSSVRTALYDSEAHVVPETMVKNERTLTATADGGAE
                     IDADEAFGQVVEAVDAVLKKTKSLKDEIEFVAASSFWHSLVGIDKKGEPTTKVFGWAD
                     TRSRKYVSVWRENFDESGVHNRTGARFHSSYWTAKLLWLQKEHEAVFKKTSKWLSFSD
                     FVALKLFGETATSVSMASGTGIFDVRKCVWDKEILSFLKIEKENLPEAAAEDSQTFRL
                     NETYARRWGKLKNAQWFPAIGDGAANNLGAGCAKKSKAALMIGTSGAMRVAFAGKPPE
                     KIPDGLWCYRIDRKRVIIGGALSDGGGLYRWLKDNFRLEKDDDKTEAEIARRAADSHG
                     LTFLPFLAGERGTGYHEAASGALLGLKSTTGAIDIVQAALESVAYRFAEIFDRLNDVC
                     KIREIIASGGALRESPVWTQIIADVLAKNLSLPDTREASSRGAVLLALETIGKIKNIE
                     EIATPRGRKFNFDKKRSAIYRKARARHERFYNLLVNSADE"
BASE COUNT         1187 a         1402 c         1274 g         1401 t
ORIGIN      
        1 aaaaactggc agcccttcga gccgacgcgc tatgagcagg tcgaagcgat gctgccgttt
       61 acggaaattc tttttaagcc gggcagcaaa tacggttatt caaatctcgg cattgttttt
      121 ttaggtcaaa ttatcgaacg attaacgggc gacgattacg aagtttatat tgacaagaat
      181 attttgaagc cgctcgaaat gtatcgaagc tatttcgacg cgacgccata tcatttgctc
      241 agatatcgct cgcacagcta tttcatcgaa aacggcaagc tcacggaagg acgcttcgac
      301 gccgattcgg gcattacggt ttcaaacagc ggtttgaatt cgcctttgcc ggatataatt
      361 aaatatctcg attttctaac cggcgacacc gaaaaaaaag aaatctacga cggaatttta
      421 aagcgctcgt cgctcgaaga aatgtttcag ccgacgattc ccgcgccgct cgacgccaac
      481 ggaaacgccg gatttacgac cgatgtcggc ttgattttct ttctcgaccg gcgcggcgac
      541 gaaacttatt tcgggcacgg cggcgaccag aacggtttta tcagctacat cgaattcaac
      601 ccgaaaaaac gcaccgcctg cgttttaatt ttcaatacca atatcatttt gcccgccggc
      661 gcgccgctcg aaaaagatac ggtcggcaaa ctgcgaaaag cgagccggca attacacgaa
      721 agcattgatt gaaaattacg aaacgccttg ctttgcgtcc cagcttcctt gcagctttct
      781 gagcaaaacg atttgcccgg cgtgatgcgc gttgtgaacg ttgatgtgcg cgacgattga
      841 gctccaaagc gattgattcg cggcggaaac cgcttcgtcg aatttgcttt cgtcggcggc
      901 ttcgagcaaa tttcgccact caatcattat cgaatcgaat ctttcggttt ccgcccgcca
      961 cgcttcttcc gaagcgtttt ccgcgcttga aaaggtttcg ttgttatcgc ttgtcggata
     1021 cacgtaatcg attcctttga aacgctttaa ataggcttcg ttgtaaaaat tcagatggct
     1081 taaaatttcc caaatcgagt tatcggcatt ttccgctttc cattttgcct gctcagccga
     1141 cacatcttta aacgcatttt tcagcgcgac aaaccagccg ttttcgtcgt agcaggcggt
     1201 aaattgttcg agtaaaactt cttttgtcgt cattacaata atttcttcgc ttttttcacg
     1261 acattctcga ccgtaaaacc ataatcgcgg aaaacgtctt cggcaggcgc gctcgcgccg
     1321 aatttatcga ccgaaagcgt gtcgccttcg agtccgacgt attttgccca gccgagcgag
     1381 acgcccgctt cgattgccag ccgcgctttg acgccggcgg gcaaaaccga ttctttgtat
     1441 ttcgccggct gctggtcgaa aaattcccag cagggcatcg aaacgacgcg cgtcggaatg
     1501 ttttgcgaat taagcagctc gcgcgcctgc atcgccagac cgacttccga gccggtcgcc
     1561 agcaaaatca gtttcggcgc ggtttccttg ccgtttttat cttcggcttc ggctaaaaca
     1621 tacgcgcctt tgtgaagccc gcgcgcgtcg ccgaattttt tgcggtcaat cagggcgact
     1681 ttttgacgcg aaagcgcgaa agccgtcggc gcgcaaacgc gttttaaagc tgcgcgccac
     1741 gcttcgcgga cttcgtgcgc gtcgcacggg cgaatcaggg cgagattcgg aatggcgcgc
     1801 agcgcggcga gatgctcgac cgattgatgc gtcggaccgt cttcgccgag cccgattgaa
     1861 tcgtgcgtga aaacgaaaac gacctgaata tttgagagcg cggcgagccg aatcgccggg
     1921 cgcatataat cggaaaacgt ctggaaagtt ccgccgtaag gaatcacgct gccgtagagc
     1981 gccattccgt tcaacgcgga gcccatcgcg tgttcgcgga tgccgaaatg aatgttgcgc
     2041 gcttcgtatc tgccagcctg gaaatctgcc gaagccttta taaatgtgtt gtttgaaggc
     2101 gtcaaatcag ccgagccgcc gacaagctgc ggaaccgttt cggcaatcgc gttgatgact
     2161 tcgcccgaat aagcgcgcgt cgctttcgct tccgcttcgt caaatttcgg cagggatttt
     2221 tcccaatcgt cgcgcaattc gcctttgcgc gtgtcgccca gagttttgcc caattccgga
     2281 aaggcttttt cgtatttttt gattaatgct tcccaatcct tttcgagctt cgcgcccgtt
     2341 ttgacggctt cgcggaaatg cgccgaaact tctttcggaa tataaaagtg cttgttttcg
     2401 tcccagccga gattgcgttt cgtttccttt acggcttctt cgcccggcgc gtccgagtgc
     2461 gccttcgacg tgccctgttt cggcatcccg aagccgataa ccgttttgac gcgaatcagt
     2521 ttcggcttgt ctttaatctt ctgcgcgtct ttgatggctt tttcaatcgc cttcaaatcg
     2581 ttgccgtcgg cgacttccaa aacgtgccag ccgatggctt caaagcgctt tgtcacgtct
     2641 tcggtaaacg ccaaatcggt cgagccgtca atcgtgatgc gattgtcgtc gtaaagataa
     2701 atcaggttgg cgagctttaa atgtccggcg agcgacgacg attcgtaact gacgccttcc
     2761 atcaaatcgc cgtccgagca aatgcagtaa ataaaatggt caatcaccgg aaaacctttt
     2821 tgattgaatt ttgccgccag atgcgcctgc gcgattccca tcccgacgcc gttggcgaaa
     2881 ccttgtccca aaggtccggt cgtgatttcc acgcctggcg tcaaaatatt ttcgggatgt
     2941 ccgggcgttt tcgaatgaag ctggcgaaaa tttttcagct cgccgagcgg caaatcatag
     3001 ccggtcagat gcagcaggct gtaaatcagc atcgagccgt gtccggcgct caaaagaaag
     3061 cggtcgcggt taaaccattt cggatttttc ggattatggc gcagaaactt tgtccagaga
     3121 acataagccg ccggagccat tccgagcggc aagcccggat gaccggaatt cgctttctgc
     3181 acggcgtcga gcgacaaagt ccggatggtg ttgatgcaaa gctggtctaa atcggttttc
     3241 gttttcggtt gcgctgcggt tgtcataatt ttttattatt tccggaataa ttcgtctatt
     3301 ttcttcggtt caaactcctc gcctttgatt actttataat ctttatcttc aaaatataaa
     3361 aaatcagccg gacttgcctt ttctgtagaa agccagaatg tagtcaattc ttcggcgtct
     3421 tcataaacta taaacgtaaa gcgagattta tcaatctttc ttaagttaaa gaatttggaa
     3481 agctctgcca gccgttgacg ttttttagct ttcgattttc ttttttccaa ttcaaaaacc
     3541 acggtggctc gataactttc attctgcctt aaaaatacga aaagattgtc gaatcttcgt
     3601 ttctcgtcat tcaaaggaga tttttcgaag cggtcaaatg gtacagggtc aataatcggc
     3661 agattttcct gcgcaaaaat cgccggacaa aaagcgaaaa ttaagattgt aataaagatt
     3721 attcggctca taacgcctta ccgcttttgc ggaaacaatt cttttatctt ttgttcaatt
     3781 tctttctgct ctattcatcg gctgaattta ccagcagatt ataaaacctt tcgtgtcgtg
     3841 cgcgcgcttt tcggtaaatc gcgcttcgct ttttatcaaa attaaatttt cgacctcgcg
     3901 gcgtggcgat ttcctcgatg ttctttattt tgccaattgt ttcgagcgca agcaaaaccg
     3961 cgccgcgact tgaagcttcg cgtgtgtcgg gcaagctcag attttttgcc agaacgtcgg
     4021 cgataatctg cgtccagacg ggcgattcgc gcagcgcgcc gccggaagcg atgatctcgc
     4081 gaattttgca aacgtcgttc aaccggtcga agatttcggc gaaacgatag gcgaccgatt
     4141 cgagcgccgc ctgcacgatg tcaatcgcgc cggtcgtgga tttcaagccc agaagcgcgc
     4201 cgcttgccgc ttcgtgataa cctgtgccgc gctcgccagc cagaaacggc agaaaagtta
     4261 atccgtgtga atcagccgcg cgtctggcga tttccgcttc ggtcttatcg tcgtctttct
     4321 ccagacgaaa attgtctttc agccagcgat acaaaccgcc gccgtcgctc aaagcgccgc
     4381 cgatgataac gcgtttccgg tcgattcggt aacaccaaag cccgtccgga attttttccg
     4441 gcggtttgcc cgcgaaagcg acgcgcatcg cgccggaagt tccaatcatc aacgccgctt
     4501 tcgatttttt cgcgcagccc gcgccgagat tgtttgccgc gccgtcgcca atcgccggaa
     4561 accattgcgc gttttttaat tttccccagc gccgcgcgta agtttcattc aggcgaaaag
     4621 tctgagaatc ttccgccgcg gcttccggca ggttttcctt ttcgattttc aaaaaactca
     4681 aaatctcttt gtcccagacg cattttctga cgtcgaaaat tcccgttccc gaagccatcg
     4741 aaacgctcgt cgccgtttcg ccgaacagtt taagcgcgac aaaatcgctg aatgaaagcc
     4801 atttcgacgt ttttttgaag accgcttcgt gttccttttg cagccagagc agctttgccg
     4861 tccagtaact ggaatgaaaa cgcgcgccgg ttcgattgtg aacgccgctc tcgtcaaaat
     4921 tttcgcgcca gacggaaacg tattttcggc tgcgcgtatc agcccagccg aaaactttcg
     4981 tggtcggctc gccttttttg tcaatcccga ccaggctgtg ccagaacgac gaggcggcga
     5041 caaattcgat ttcgtctttt aagcttttcg tttttttcaa aaccgcgtcg accgcttcga
     5101 cgacctgccc gaaggcttca tccgcatcga tttccgcgcc gccgtcggcg gtggcagtga
     5161 gggtgcgttc atttttcacc atcgtttcag gcacaacgtg cgcttccgaa tcgtagagtg
     5221 cggtgcgaac gctggaagtt ccgatgtcaa tagctaggac gtgc
//
LOCUS       DASWNE010000542         2982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_688628, whole genome shotgun sequence.
ACCESSION   DASWNE010000542 DASWNE010000000
VERSION     DASWNE010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688628"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="VGB68_10775"
     CDS             complement(<1..847)
                     /locus_tag="VGB68_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049762.1"
                     /translation="MKSRREFLKTSCRALSMTALATQLGHFGLLSAFAQKSPSSRDEI
                     APPNDYKALVCILMFGGNDGNNTIIPNHNDANVSNYAAYNAARAPQGLALPQNSLLPI
                     TVPRVGGLRYGLHPGFGTIAGGINGGLHELWALGKMAVVSNVGTLVQPLTRQQYQSRS
                     APRPLQLFSHTDQMAQQQSARADVTSLSGWGGRISDKMTVSANPERLVPTITGTGGSL
                     FAFGDTTQPLSIGPAPTSLSNILAIAGDTNTPVANARLNSLIQSMNLSSTSELVVASS
                     QINRTA"
     gene            complement(964..>2982)
                     /locus_tag="VGB68_10780"
     CDS             complement(964..>2982)
                     /locus_tag="VGB68_10780"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEY0049763.1"
                     /translation="ENWNGNLPKASQQAFRPGQKSVITLFVSNLDLLEGEGANAFRVY
                     LNHRNGRTYQFPVEELTPITRTVYALKIRLFDQGLRGQPPADGDALIYLTWRGLASNQ
                     LKIGLGRTGGNINVPVAAKSVSRAVEAPPASELVGYRWSADRKRFLEQATFGPSPEMD
                     ARVRRFGLRTWLGEQLEAPYPTIPYPDIPLMPTIPPSGCSLTTFPHCYRERYTMQPVQ
                     QWFFKEAFYGNAQLRHRTAWALSQIWVTSGETIKQSSHMIAFHKVLSKNTFGNYRALM
                     YDATLNPAMGDYLDMIRSTKISPNENYAREILQLFSIGLYMLNQDGTLKLDNQGNPIP
                     TYSQETINNFSKLFTGWTYCHDPAVCPQATPGTLNFKDPMILNPDNHDTSAKTLLNYP
                     NAINQNIPACSDCTDSTRTWIYANDTLQQAMNNIFYHPNVAPFVSKLLIQHLVTSDPS
                     PAYVGRVAAIFNNNGQNVRGDLRAVVSAILLDPEARGNFKSDPRYGKLREPVQMVTNI
                     ARHYNAKSYNGEELSDGGLGETMRGMGQTPFYAPSVFNYFPPDYIVPGTTLLAPEFNL
                     LNTSTGIKRINTLYILIFEGITPNATDSLKGTSIDVGEMVPFSVADATGNLLVDAVNT
                     KMMHGTLTPELRSLVLNAVKTVPASDPVLRAKTAIYLVAASSQYQVQR"
BASE COUNT          665 a          803 c          822 g          692 t
ORIGIN      
        1 tagccgtccg gttgatttga ctggaagcta cgaccagttc ggacgtcgag cttaaattca
       61 tcgactgaat cagcgaattc agacgcgcat tggcaaccgg cgtgttcgta tcgccggcga
      121 tggcgagaat gttgctcaga gatgtcggcg cgggtccgat tgataacggc tgggtcgtat
      181 ctccaaacgc gaaaagcgag ccgcccgttc cggtaatggt cggaacaagc cgttccgggt
      241 tagcggaaac agtcatttta tcggaaattc gcccgcccca gccggacagg gaagtgacgt
      301 cggcgcgcgc gctttgctgc tgcgccatct ggtctgtgtg cgagaataat tgcagcgggc
      361 gcggcgctga cctgctctga tattgctggc gcgttaaagg ctgcaccagt gtgccgacat
      421 tgctgacaac cgccattttc ccgagcgccc agagctcgtg caaaccgccg ttaatgccgc
      481 ccgcgattgt gccgaaaccc ggatgcaagc cgtatctcaa accgccgacg cgcggaaccg
      541 taatcggcaa aagcgagttt tgcggcagag ccaagccctg cggagcgcgc gccgcgttgt
      601 atgccgcgta attgctgaca ttcgcatcgt tgtgattcgg aataatcgta ttgtttccgt
      661 catttccgcc aaacatcagg atgcagacca gcgccttgta atcattcggc ggcgcgattt
      721 cgtcgcggct gctcggcgat ttctgcgcaa aggcgctcaa caagccgaaa tgcccgagct
      781 gcgtcgccag cgccgtcata ctcaaagcgc gacacgatgt ttttaaaaat tctcttctgc
      841 ttttcataat tctcttaaat tagttgaccg agttgactgg aagttgaccg agttgaccga
      901 gttagtcgag tgtttctgaa aactcggtca actcactaaa ctttcagtca acttccagtc
      961 aacttatctt tgcacctgat attgcgacga agccgcaacc agataaatag ccgttttcgc
     1021 tctcagaacc gggtcggaag ccggaacggt cttgaccgca tttaacacca ggctgcggag
     1081 ctcgggcgtt aaagtgccgt gcatcatttt ggtgttgacc gcgtcgacaa gcagattgcc
     1141 ggtcgcgtcg gcgacggaaa aaggaaccat ttcgccgacg tcaatcgaag ttcctttcag
     1201 cgaatcggtc gcgttcggcg tgatgccttc gaatatcaga atgtaaagcg tattgatgcg
     1261 cttaatgccg gtcgacgtat tcaaaagatt gaattccggc gcgagcagcg tcgttcccgg
     1321 cacgatataa tccggcggga aatagttgaa aacggacggc gcgtaaaaag gcgtctgtcc
     1381 cattccgcgc atcgtttcgc ccagcccgcc gtcgctcagt tcttcgccgt tgtaggattt
     1441 cgcgttgtaa tgccgcgcga tgttcgtcac catctgcacc ggctcgcgca gtttgccgta
     1501 acgcgggtcg gatttaaaat ttccgcgcgc ttccgggtcg agcagaatcg ccgagacgac
     1561 cgcccgcaaa tcgccgcgca cgttttgacc gttgttgtta aagattgccg cgacgcgccc
     1621 gacataggcg ggcgacgggt cgctcgtcac cagatgctga atcaaaagct tgctgacgaa
     1681 aggcgcgaca ttcggatgat aaaaaatatt gttcatcgcc tgctgcaatg tatcgttggc
     1741 gtaaatccag gttcgggtcg aatcggtgca atcgctgcaa gccggaatgt tttgattgat
     1801 ggcgttcgga taattcaaaa gggttttcgc gctggtgtcg tgattgtcgg gatttaaaat
     1861 catcgggtct ttgaaattca aagttccggg cgtcgcctgc gggcaaaccg ccgggtcgtg
     1921 acaataagtc cagccggtaa agagcttcga aaaattattt atcgtctcct ggctgtaagt
     1981 cggaatcggg ttgccttgat tatccagttt gagcgtgccg tcctgattga gcatatacaa
     2041 gccgatggaa aacaactgca aaatctcgcg cgcgtaattt tcgttcgggc tgattttcgt
     2101 gctgcgaatc atatccagat aatcgcccat cgccggattc agcgtggcgt catacatcaa
     2161 agctcgataa ttgccgaaag tatttttcga taacacttta tgaaaggcaa tcatatgact
     2221 cgactgttta atcgtctcgc ccgaagtgac ccaaatctgg ctcaatgccc aagccgttcg
     2281 atgacgcagc tgcgcgttgc cgtaaaacgc ttctttaaaa aaccattgct gaaccggctg
     2341 catcgtgtat cgctcgcgat agcagtgcgg aaaagtcgtc agcgagcagc ccgagggcgg
     2401 aatcgtcggc atcagcggaa tgtcgggata gggaatcgtc ggatacggag cctcaagctg
     2461 ctcgccgagc cacgtccgca aaccgaagcg ccgaactctc gcgtccatct ccggcgacgg
     2521 tccgaaagtc gcctgttcca gaaatctttt gcggtcggca gaccatcgat agccgaccaa
     2581 ttcggaagcc ggcggcgctt cgacggcgcg cgagacggat ttggcggcga ccggaacgtt
     2641 tatgttcccg ccggttctgc cgagaccgat tttcagttga ttgctcgcca gaccgcgcca
     2701 cgttaaataa atcagagcat cgccgtcggc gggcggctgc ccgcgcaaac cctggtcaaa
     2761 caggcggatt ttcagcgcgt aaacagttcg ggtgatcggc gtcagctctt cgaccggaaa
     2821 ttgatatgtc ctgccgttgc gatgattcag ataaacgcga aaagcgtttg cgccttcgcc
     2881 ttcgagcaaa tccagattcg acacaaaaag cgtgataacg cttttttgac ccgggcgaaa
     2941 cgcctgctgc gaggctttcg gcagatttcc gttccagttt tc
//
LOCUS       DASWNE010000543         4897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_123328, whole genome shotgun sequence.
ACCESSION   DASWNE010000543 DASWNE010000000
VERSION     DASWNE010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123328"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VGB68_10785"
     CDS             <1..307
                     /locus_tag="VGB68_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049764.1"
                     /translation="PTPIAGGEGGLGNAVGTSRTPTTFNLDLGVYFPIKFGEKRELRF
                     TADWFNVTNTQRAVKLDETFLINSGVTNVAPVANPFFGTGQIFQYPSSLRLGAKFRF"
     gene            423..1478
                     /locus_tag="VGB68_10790"
     CDS             423..1478
                     /locus_tag="VGB68_10790"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0049765.1"
                     /translation="MSANNRFSRVTVIESGVAFIALLLIFGAAVADGQGRGISIGGGV
                     NKIQGRVRFPSNYPATSVRVRLENPAISSVMTVTDSEGMFYFNGLGAGQYTVIVEAGE
                     DYEVFREVVDIDAPVSLDRTIYIPPSPRTFNVMADLRPKGAIRNRPGVINASLAAVPK
                     TALKEFKKALEAQNKGETNLAIEKLNEAIKLHPQFFEAHAELGFLYLKTNQLDKAVES
                     LRAALELNDKNPNIRLNYAVALLNKKEMAEAEKEFREVIKADKTAAVAHMYLGIALLG
                     LKRVDEAEALFLQAISIKDDEKLAQAHRYLGGIYWGKGKYARAADELEKYLKLAPKAT
                     DAEKIRETVKNLREKTK"
     gene            1793..>4897
                     /locus_tag="VGB68_10795"
     CDS             1793..>4897
                     /locus_tag="VGB68_10795"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0049766.1"
                     /translation="MIKRIASLFSVLSMLFVLGSFAFGQETTGNLEITVKDPSGAVVP
                     NVTLTIASAGNTTGFRRTITTDENGFQRVMNVPPGSYTVTAGATAGFQERTVTDIQVA
                     LGQTSPVLIEISTTVGGTVTITETDTPPIDPTSSKIQTTISAQTAELLPKGTNFSSIL
                     KVSPATRPEPRSGQFQVDGASGSENTFIVDGQEVTNVRTGVLNTNSNLPFQLIQEVQI
                     KSSGFEAEFGGATGGVISVVTKGGSNQWRGEFGSQFEPGKLQARGRNLLITRNNAPFY
                     IAQGKDAYLGFFPTANLGGPILKDRLWFFGSYTPQIFTRNRTINYAELRNSSNVITRA
                     ASSETYRGKQTNEYAFVRLDAQPFSRLRLNGTYTYNPIAVTGTLPSYNTIFDAIPSSE
                     AVGLSGANFYNQTGGRQNSQSVTGQATWVPTNDFTLNVRGGHYYLNEKLGTYGFGDPT
                     RPRVLCSASSPTQFPGDFGCIRGQSNGQPFFSGTVYDATSRNTLDADATYIANFAGRN
                     EFKGGYQYNGIANQLLSQDVDQIVLRFGQTIGSYSGRNLPSTPGALGAGTLIRYRESG
                     DVSSKNEGIYIQDKWQPFNRLTLNLGVRAERENVPSFTEGRPGLKFDFMDKIAPRIGV
                     AYDLTGDGKTKVTAFYGWFYDRFKYELPRGSFGGAFYHQFFFEILPGTNLGSYDTPGD
                     ILGNGTGIIGGSCPTGANAPTTPIFGRVRCDIDYRVPSNSGLDINSYGGIDPDLKAFR
                     QSEMTLTFERDLGRDFVFSARATRKNVDRTVEDAGFITPGGSESYVIGNPGTGLHGQL
                     LASQGLLPATPQRLYRALEFRLDKRFANNYYFNLNYTFSRLEGNYSGLASSDEDGRLS
                     PNVNRYFDLPHAGFTVKGGPDNGVLSTDRPHVLKFAGAYSLDWNDRFGFGSNQTTEFQ
                     VFTTAQSGSPITTTVDVLGIDTIILTERGDAGRTELYTNTDFAIRHRYRFGRDNRFTL
                     IGEIDVLNVFNQANVLNRDNLIDLTDYDITNPLLGLITAAEADLPNAYQLALGRFQRN
                     GAPIL"
BASE COUNT         1255 a         1243 c         1229 g         1170 t
ORIGIN      
        1 cccgaccccg attgcgggcg gcgaaggcgg tctcggaaac gctgtcggca ccagccgtac
       61 gccgaccacg ttcaatcttg atttgggtgt ttacttcccg atcaagtttg gcgaaaaacg
      121 tgagctgcgt ttcactgccg actggttcaa cgtcacgaac actcagcgtg ctgtcaagct
      181 cgatgaaaca ttcctgatta acagcggcgt cacgaacgtt gcgccggtgg caaatccgtt
      241 cttcggaact ggacaaatct tccagtatcc gtcgtcacta cgcttgggag cgaaattccg
      301 cttctaataa cggaaatttc gagccaattt gaaaagggaa agtgttctac actttccctt
      361 tttgtgttaa tataaggaaa attcaaaaac aactttttcc ctgtcaggta aaggaggcta
      421 ctatgtcggc aaataatcgt ttctcgcgtg taacggtgat tgaatccgga gtggcgttta
      481 tcgctctttt attaattttc ggcgctgccg tcgccgacgg gcagggcaga ggcatcagca
      541 tcggcggcgg agttaacaaa attcagggac gcgtccgctt tccttcaaac tatccggcga
      601 cctcggttcg cgtcaggctg gaaaatcccg ccatcagcag cgtgatgacc gtcaccgatt
      661 cggaaggaat gttttatttt aacggtctgg gcgcgggtca atacacggtc atcgtggaag
      721 ccggcgaaga ttatgaggta ttccgcgaag tcgtcgacat cgacgcgccc gtgtccctcg
      781 accgaaccat atacattcct ccttcgccgc gcacgtttaa tgtaatggct gatttacgac
      841 cgaaaggagc gataagaaat cgaccgggcg tgattaacgc ctcgcttgcc gccgttccga
      901 aaacagctct gaaagaattt aaaaaagccc tcgaagctca aaataaaggc gaaacgaatc
      961 tggcaatcga aaaactcaac gaagccatta agctgcaccc gcaatttttt gaagcccacg
     1021 ccgagctcgg ctttttatac ctgaagacaa accagttgga taaagccgtc gaatcgctgc
     1081 gcgcggcgct tgagctgaac gataaaaatc cgaacatccg acttaattac gccgtcgctc
     1141 tgctgaacaa aaaggagatg gcggaggctg aaaaagaatt tcgcgaagta atcaaagccg
     1201 ataagacggc ggcggtcgcg catatgtatc tcggcatagc tttattggga ctcaagcgcg
     1261 ttgacgaagc ggaagcgctg tttctgcaag ccatcagcat caaagatgac gagaaactcg
     1321 ctcaggcgca ccgctatctg ggcggcatct attggggaaa aggaaaatac gcgcgagcgg
     1381 ctgacgaact ggaaaaatac ctgaagctgg ctccgaaggc gaccgacgcc gaaaaaattc
     1441 gtgaaaccgt caagaatctg cgcgaaaaaa caaaatagcc gcgcttgaat acgcccgccc
     1501 gtcgtctgca gatttttcga ttaaagcgcg gcatccgaaa tttcgtgatt taatatattg
     1561 aataaaatcc aaaatcttga agtaaaatcc cgctccgggt tagggttttt attaaccgaa
     1621 aattacataa acccctttat ttcaagagat tacttaaata aatccaacct tcgaatcttt
     1681 ggcacttgaa ttgttattaa taggtcagtt gagtagttta ttcaaaaaaa tctctcaggc
     1741 aagaggctgg gtcaactact acttagagta ataatttcaa gaggagtaaa ttatgattaa
     1801 aagaatagct tctttgtttt ctgttctgtc aatgctgttc gtattaggct cattcgcctt
     1861 cggacaggaa acgacgggaa acttggaaat caccgttaaa gaccctagcg gcgcggttgt
     1921 cccgaatgtg actttaacaa tcgccagcgc aggcaacacg acgggattta gaagaaccat
     1981 aacgacggat gaaaacggat ttcagcgcgt tatgaatgtg ccgccgggca gttatacggt
     2041 cactgcgggc gcaactgccg gttttcagga aagaacggtt accgatattc aagtagcact
     2101 tggacagacg tcgcctgttc tgattgaaat cagcacgact gtcggtggaa cggtaacgat
     2161 taccgaaacc gacactccgc cgattgaccc gaccagcagc aaaattcaaa cgaccatttc
     2221 ggctcagact gcggagcttc ttccgaaagg aacgaacttc tccagcattc ttaaagtctc
     2281 tccggcgaca cgtcctgaac cgcgttcggg tcagtttcag gttgacggag cctcgggttc
     2341 ggaaaacacc tttattgttg acggacagga agtaaccaac gtccgcaccg gtgttttgaa
     2401 cacgaacagc aacctgccgt tccagcttat tcaggaagtt cagattaaat cttccggttt
     2461 tgaagctgaa ttcggcggcg cgaccggcgg cgtgatttcc gtcgtgacga aaggcggcag
     2521 caaccagtgg cgcggcgagt tcggttcgca attcgaaccg ggcaaactgc aagccagagg
     2581 acgcaacctt ttaattaccc gcaacaatgc gccgttttat atcgcgcagg gcaaagacgc
     2641 ctatctggga ttcttcccga cggcgaatct gggcggtccg attctcaaag atcgtctctg
     2701 gtttttcggc agctatacgc cgcagatttt taccagaaac cgaacgatta actacgcgga
     2761 gctgagaaat tcttccaacg taattacccg tgctgcgtcg tccgagacgt atcgcggcaa
     2821 acagaccaac gaatatgctt tcgttcgtct ggatgcacag ccgttcagca gacttcgctt
     2881 aaacggaact tacacctaca atccgattgc agtgactgga acgcttccct cgtacaacac
     2941 gattttcgat gctattcctt cgagcgaagc agtaggtttg tcaggcgcaa acttttataa
     3001 tcagaccggc ggtcgtcaga attcccagag cgttaccgga caggcgacgt gggtgccgac
     3061 caatgacttt acccttaatg tacgcggcgg acactattac ttaaatgaaa agctgggaac
     3121 ctatggtttc ggcgacccga cccgcccgcg cgttctttgc agcgcgtcga gcccgacgca
     3181 gttccccggt gacttcggat gtattcgcgg acaatcaaac ggacagcctt tcttcagcgg
     3241 caccgtttat gacgcgacaa gccgtaacac cttggatgcg gacgctactt atattgccaa
     3301 ctttgcaggc agaaacgagt tcaaaggcgg ttaccagtac aacggaatcg cgaaccagtt
     3361 gctgagccag gacgtagacc aaatcgtttt gcgtttcggt cagacgattg gttcttattc
     3421 aggtcgtaac cttccttcga cgcctggtgc tttaggtgcc ggaacgctga ttagatatcg
     3481 tgaatcgggt gatgtcagca gcaaaaacga aggcatctat atccaggaca aatggcagcc
     3541 gtttaatcgt ctgacgttaa acctgggtgt tcgcgccgaa cgcgaaaacg ttcccagctt
     3601 taccgaagga agaccgggct tgaaattcga cttcatggac aaaatcgcac cgcgtatcgg
     3661 tgttgcctat gatttgaccg gcgacggcaa aacgaaagtc accgctttct acggctggtt
     3721 ctacgatcgt ttcaaatatg agctgccccg cggttcattc ggtggtgctt tctatcacca
     3781 gttcttcttt gaaatccttc cgggaaccaa tcttggaagc tacgatactc cgggagatat
     3841 tctcggcaac ggaaccggca ttatcggcgg cagctgcccg accggagcca atgcgccgac
     3901 gaccccgatt ttcggacgcg ttcgctgcga tatcgactat cgtgttccgt cgaactcagg
     3961 tctggacatc aactcctacg gcggtatcga tcctgatttg aaggcattcc gtcagagtga
     4021 aatgacgctt acctttgaac gcgatttagg aagagacttt gttttcagcg cccgcgcaac
     4081 ccgtaaaaac gttgacagaa ctgttgaaga tgccggtttc attacgccgg gcggcagtga
     4141 atcatacgtt atcggtaatc cgggaaccgg actgcacggt cagcttctgg catcgcaggg
     4201 attgcttccg gctacgccgc agcgtttgta tcgcgcttta gagttccgac tcgacaagcg
     4261 ttttgccaac aactactact tcaacctgaa ctacaccttt agccgtctgg aaggaaacta
     4321 ctccggcttg gctagctcgg atgaagatgg acgtttaagc ccgaacgtta accgctattt
     4381 cgacttgccg catgccggat tcacggttaa gggcggaccg gataacggtg tattgtcgac
     4441 cgaccgtccg cacgtgttga agtttgccgg cgcttacagc ttggattgga atgacagatt
     4501 tggtttcgga agcaatcaga cgaccgaatt tcaggtcttc accactgctc aaagcggttc
     4561 gccgataacc accacggtcg atgtgttggg tattgacacg attattttaa ccgaacgcgg
     4621 cgacgcgggt cgcacggaac tgtataccaa tactgacttt gcaatccgtc accgctatcg
     4681 tttcggacgc gataatcgtt tcaccctgat tggtgaaatt gatgttctga acgtgttcaa
     4741 ccaggcaaac gttttaaacc gtgataacct gatcgactta acggattatg atattaccaa
     4801 tccgttgctt ggtcttatta cggctgctga agcggacttg ccgaatgcgt atcaattggc
     4861 tctcggacgt ttccaaagaa acggagcgcc gattctg
//
LOCUS       DASWNE010000544         2990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_688642, whole genome shotgun sequence.
ACCESSION   DASWNE010000544 DASWNE010000000
VERSION     DASWNE010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688642"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VGB68_10800"
     CDS             <1..577
                     /locus_tag="VGB68_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049767.1"
                     /translation="ENWTAERVSSTTRFKVNSLVRFTIESPRSGFLYIINREVYTDGT
                     RGEAKLIFPTLKANAKGENRVVAGSLVEIPHSRARTPYFRVASEEPDYAGEEVVVIIS
                     PVRLPGMKIDLEPSPVAVEKLEKWLAEWGATVDIFEAADGEGVALTQVEEEAAQTRAL
                     TPEEPSPQTIYSLKARANQPLLIVFQMQARK"
     gene            625..2430
                     /locus_tag="VGB68_10805"
     CDS             625..2430
                     /locus_tag="VGB68_10805"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0049768.1"
                     /translation="MTKPNMLTNGKKLDGRYILREEIGSGGFGAVYRATDTRFSGNNQ
                     VAIKQIPLNNAEAATAFRHEANLLYNLSHPNLPKVTNCFQEDGASFIVMEYISGDDLS
                     ESLKKGKRYGVAESLEITDAVLDALEYLHSFTIFHRDIKPHNIKISGNGKIYLLDFGT
                     AKGSFDETTLPRIGQSITGFTPFYAPLEQVLRVDPNSYLLLKSLETPRLEEFISRKTD
                     ERSDIYSLGATVYHLLTGYSPEQATATTRAFSLWSGKPDPLPSCQIVNPEIPERLAQI
                     VHRCLEIEPEKRFQTAAELRRELKNPSPTNSSPKVSEQMTVALPADARNRLNAESIST
                     RAANLKSEPIARFSFEVENEAERETDLRPIEDLSGERRKLKQAKPKSKMPLYLGLLVL
                     SVVLGVLAGWLWWRNSAETPPSVAENPPAKNARNLSYSLLVQKMRDGKKFQEPFESSG
                     QEIFETGYQFQMRLTPPADGYLYIFAEGINERQEKVFNLIFPTPQRNQGSAAVSAPRQ
                     YETGWNQFDGKTGTESFWMIWNKTQPEIAEKARQNAFGNAGVVTDGNLKNLLKSYLVS
                     ESKNYAEASKETGKKITKVGFEGDGLVYRIHLEHR"
     gene            2590..>2990
                     /locus_tag="VGB68_10810"
     CDS             2590..>2990
                     /locus_tag="VGB68_10810"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEY0049769.1"
                     /translation="MRSSSFKNKFPLRLASLLILFSFMFSLLPPRSAQGQTAPEKTRE
                     LIADEKPEKEQKRLALVIGNGAYQHVASLKNPVNDAMDITKALDELGFEVISGVNQNK
                     AQMKKLIREFGDKLMIQKGVGLFFFAGHGVRS"
BASE COUNT          793 a          748 c          816 g          633 t
ORIGIN      
        1 ggaaaactgg acggcggaac gcgtcagctc gaccaccagg tttaaagtca acagcctcgt
       61 acgttttacc atcgaatcgc cgcgctcggg ttttttgtat atcattaacc gcgaggttta
      121 cacggacggc acgcgcggcg aagcgaaatt aatttttccc actttaaaag cgaacgctaa
      181 aggcgagaat cgagtcgtcg cgggcagcct ggtggaaatt ccgcattcgc gtgcccgcac
      241 gccttatttc cgcgtcgctt cggaagagcc ggattacgcc ggagaagaag tcgtcgtgat
      301 tatttcgccg gtcaggcttc cgggtatgaa aatagacctc gagccttcgc cggtcgccgt
      361 cgaaaaattg gaaaaatggc tggctgaatg gggcgcgacc gtcgatattt tcgaggcggc
      421 ggacggcgaa ggcgtcgcct tgacgcaagt tgaagaggaa gcggctcaaa cgcgcgcttt
      481 gacgccggaa gaaccatcgc cgcagacaat ctacagcctc aaggcgcggg cgaatcagcc
      541 gcttcttata gtgtttcaaa tgcaagccag aaaatagagg aaagcgcgaa agcgcgaaag
      601 cgaaaacgaa gccgccgcaa ccgattgaca aaaccgaata tgctgaccaa cggaaaaaaa
      661 ctcgacgggc gttacattct tcgcgaagaa atcgggagcg gcggcttcgg cgcggtttac
      721 cgggcgacgg acacgcgttt tagcggcaat aatcaggtgg cgataaagca gattccgctc
      781 aataacgcgg aagcggcaac agcttttcgc cacgaagcca atcttcttta caacctttcg
      841 cacccgaatc tgccgaaggt gacgaactgt tttcaggaag acggcgcgag ctttatcgtg
      901 atggaatata tttcgggcga tgatttgtcg gaaagcctga aaaaaggcaa acgctacggc
      961 gtcgccgaat ctctggaaat taccgacgcc gtcctggacg cgctcgaata cctgcactcg
     1021 tttacgattt ttcaccgcga catcaagccg cacaatatca aaatctccgg aaacggcaaa
     1081 atttatctgc tcgatttcgg cacggcgaaa ggcagctttg atgagacgac tctgccgcgc
     1141 atcgggcaga gcattaccgg atttacgcct ttttacgcgc cgctcgaaca ggttttgcgc
     1201 gtcgacccga attcttattt gcttttgaag tcgctggaaa cgccgcgtct ggaagaattt
     1261 ataagccgga aaaccgacga gcgtagcgat atttacagcc tcggcgcgac cgtctatcat
     1321 cttctgaccg gttattcgcc ggagcaagcg acggcgacga cgcgcgcttt ttcgctctgg
     1381 tccggcaagc ctgaccctct gccgtcgtgc caaatcgtca atccggaaat tcccgagcgt
     1441 ctggcgcaaa tcgttcatcg ctgcctggaa atcgagccgg agaaacgttt tcagacggcg
     1501 gctgaattgc gtcgcgagtt gaaaaatccg tcgccgacaa attcgtcgcc gaaagtatca
     1561 gagcaaatga cggtcgcgct cccggctgat gctcggaacc gattaaatgc ggaatcgatt
     1621 tcgacgcggg cagcgaattt gaaatccgaa ccgattgcgc gcttctcgtt tgaagttgaa
     1681 aatgaagcgg aaagggaaac ggatttacga ccaatcgaag atttatcggg cgagcggcgg
     1741 aaacttaaac aggcaaagcc gaaatcgaaa atgccgcttt atctcggatt gctggtttta
     1801 tcggtggttt taggcgtttt agccggttgg ctgtggtggc gaaattcagc ggaaacgccg
     1861 ccgtcggtcg ccgaaaatcc gccggcgaaa aatgcccgta atttgagcta ttcgctgctg
     1921 gtgcagaaaa tgcgcgacgg caaaaaattt caggagccgt ttgaatcgag cgggcaggag
     1981 atttttgaga cgggctatca atttcagatg cgcctgacgc cgcccgccga cggttatctt
     2041 tatatttttg ccgagggcat caacgagcgt caggaaaaag ttttcaacct gatttttccg
     2101 acgccgcagc gaaatcaggg ctcagccgcc gtttccgcgc cgaggcaata tgaaacgggc
     2161 tggaatcagt ttgacggcaa aacgggaacg gaaagcttct ggatgatttg gaacaaaact
     2221 cagccggaaa ttgccgaaaa ggcgcgccag aacgctttcg gaaacgcggg cgtcgtaacc
     2281 gacggcaatt tgaaaaatct gctgaaaagt tatctggtaa gcgagagcaa aaactatgcg
     2341 gaagcatcga aagaaaccgg taaaaaaatc acgaaagtcg gttttgaagg cgacgggctg
     2401 gtttatcgga ttcatctcga acaccgttaa aacaaggcga atgcttgacg gcggagagat
     2461 ttacacatag aattatgcca cccaaaaatc ggacggcaac tgtccggtca agctgaaatt
     2521 aaaatctgaa actgaaaaca cgctttcacc acagacgtgt ttccgcacgt tgaaacagga
     2581 gttctcgcta tgcgcagttc gtcattcaaa aataaattcc cgcttcgcct cgcgagcctt
     2641 ttaatattgt tttcgtttat gttttcgctg ctgccgccgc ggtcggcgca gggacaaacc
     2701 gcacccgaaa aaacgcgcga gcttattgcg gatgaaaaac ccgagaaaga acaaaagcgt
     2761 ctggcgctcg tcatcggcaa cggcgcgtat caacacgtcg cttcgcttaa aaatcccgtc
     2821 aatgatgcga tggatataac gaaagcgctc gatgagctgg gttttgaggt aatatcgggc
     2881 gtcaatcaaa acaaggcgca gatgaaaaaa ctcatccggg aattcggcga caagctgatg
     2941 atacaaaaag gcgtggggct tttctttttt gccggacacg gcgtgcgctc
//
LOCUS       DASWNE010000545        11202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_266160, whole genome shotgun sequence.
ACCESSION   DASWNE010000545 DASWNE010000000
VERSION     DASWNE010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11202
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266160"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..646
                     /locus_tag="VGB68_10815"
     CDS             131..646
                     /locus_tag="VGB68_10815"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0049770.1"
                     /translation="METERLVLRRYAEKDKPDFIALFTDAAVMKYVGDGVLTGEQAEA
                     FWQKLFEKLYPQNFNIWAISAKEDSAYIGHAGIYPRPSHKEDWEFVYFLNQKAWGKGY
                     ATEIARRIISFAFEDLNLPEVFATVDDAHSASIHVLKKAGMSFKNYEYDEKGRFSVYS
                     LRKRNRADTEA"
     gene            complement(772..2667)
                     /gene="asnB"
                     /locus_tag="VGB68_10820"
     CDS             complement(772..2667)
                     /gene="asnB"
                     /locus_tag="VGB68_10820"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEY0049771.1"
                     /translation="MCGIAGWANLDDKSSSEGGEAVLHAMCERMKHRGPDSEGLWTGE
                     SVALGMRRLSIIDLQTGEQPFWNEDKSIVVVMNGEIYNFRELRKELERRGHEFHSHSD
                     TEVLPHLYEEYGEAMVEHLNGMFAFALWDLRRKKLLIARDRFGEKPLYYGVFAGKLIF
                     ASEPKVLLRHPAVEPEINPEALRQYLCFDYVPAPLSIYKNIYKLPAAHLLTVENGEVK
                     TRRYWNLSFHKNGNTPTVETAAKELREIISDAVRMRLVSDVPLGILLSGGVDSSTVAA
                     FATQHATEKVKTFSIGFEEDSFDESKFARQVAEHLGTEHYEEKLSVEKAADLISEIGT
                     WLDEPVSDGSLIPTFLLSRFVRKHVTVALGGDGGDEIFAGYPMYFGHKMARVYDRLPR
                     FLRAGLIEPIVNNLPVSTKNLSFDYKAKRFVAASKYDAVTRHHSWFGSFSIDAQNDLL
                     AKDFPAGTSGDIYKGAKDLLKITDATDEIEQMQFLDMNFYMAEDILTKVDRASMAVSL
                     EVRAPFLDSRVAEYAASLPLEYKLKGSKGKYILKKAVEDLLPKTILQRPKKGFGIPIA
                     DWLKGRLNPLMRDLLAPERLENQGLFDAGYVQKLIAEHEAGAASHHKQLWTLLVFQLW
                     FDNFLKK"
     gene            complement(2691..3698)
                     /locus_tag="VGB68_10825"
     CDS             complement(2691..3698)
                     /locus_tag="VGB68_10825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0049772.1"
                     /translation="MKMISVQTELTDLPKTDAMPELSLFLPVLNEEENLRPMHAKIRE
                     ALLSLGKTAEVIYVDDGSTDKSLQILREIAEQDEKVRVISLRRNYGQTAAMSAGIDAA
                     KGEILIPMDADLQNDPKDIARLLEKLDEGYDVVSGWRKNRQDKLISRKIPSQIANKII
                     SWIGGVPLHDYGCSLKAYRREVIQDVRLYGEMHRFIPIYASWAGARVTEIPVDHHARI
                     MGKSKYGISRTIKVVFDLMTIKFMASYQTKPIYVFGTFGMIAFLLSIVAAIWAFFLKF
                     VEGVSFILTPLPIIAIVMLAISVQFFLMGLLAEMLVRTYHESQNKAIYTVRERIGFDD
                     K"
     gene            complement(3916..4677)
                     /locus_tag="VGB68_10830"
     CDS             complement(3916..4677)
                     /locus_tag="VGB68_10830"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0049773.1"
                     /translation="MSSALPQEMQQHTYAIMNRVEDSHWWFVGRRLILESFLRQIHSA
                     LGARDSALKILDVGCGTGANLEMLAQFGAAEGVDVSDSALEFCRAKGLKTHKGLAEKL
                     PFDDESFDLVTALDVVEHLDDDAAGLKEMHRVLKSGGRTLIFVPAFMWLWGVQDDISN
                     HRIRYTKKQIVERLEKAGFEIERATYANWTFFAPILAGRTLMKITGIKPESENNVNVS
                     ALNGVFGKLFGAERFWLKNFDFPFGVSIVIVAKKS"
     gene            complement(4786..5430)
                     /locus_tag="VGB68_10835"
     CDS             complement(4786..5430)
                     /locus_tag="VGB68_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049774.1"
                     /translation="MRFDKDIAKKYRRSIEEAFATILEKGSDFHREMMEIIIESEMLV
                     CVFPVSRVNASGITGIINARKTNDKIDSEILSPREALGEVFITIAEETIDTGGQRGCE
                     GTFVHEGRHAFDFAQTIASFSDADVNPLSLYDPTLYELEWLAHKTAGDYMLQIDRDEY
                     LQEGLDLMILGRGGDGRCFVDDDGIKRRLRESYGLEEHGNRGSSVSQMFGLRQR"
     gene            complement(5470..6000)
                     /locus_tag="VGB68_10840"
     CDS             complement(5470..6000)
                     /locus_tag="VGB68_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049775.1"
                     /translation="MSFNFIDLDDFRDLMSEEIKFDLENNALYLSKGLSSRGRSLWQQ
                     LLTEAILRHDEVWLTEQIRNQDLLNPTESYQRNGETLLRNTRKNAPEILAEGEFNKFY
                     ARAVCRKAIDDGKRIRIYRARESNNPRPESEQKIGQIINPQSLLEDLRANKTAEPFFG
                     VALINSGLSVTIEDGD"
     gene            complement(6028..7017)
                     /locus_tag="VGB68_10845"
     CDS             complement(6028..7017)
                     /locus_tag="VGB68_10845"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0049776.1"
                     /translation="MKEKLLDLLACPTCGGDILLSYAGKYEDREIIEGVLSCKKCLRE
                     YKIARGVPRFADLSKIEQDKADTAENFGWQWTHFTQADEKYAEQFLGWLQPVKEDFFK
                     DKFVLEGGCGKGRHTSLAANWGAREVVGIDLSAAVEPAFQATRALPNAHIVQADIFKL
                     PFKRAFDYAFSVGVLHHTPDPKGAFLALASKVKNGGHISAWIYGAENNEWITRYVNPI
                     REGFTSKISQPALYQLSKLPTLGVFLASKLIYKPLDKTSFGKKLFYHEYLSHLGTFGW
                     REQHNIVFDHLVAPTAFYISRDEFTRWWQEINARDVEIIWHNQNSWCGFGKII"
     gene            complement(7243..7719)
                     /locus_tag="VGB68_10850"
     CDS             complement(7243..7719)
                     /locus_tag="VGB68_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049777.1"
                     /translation="MANKLEVSFNSPQCGWMSIGFDDGSSEFHTTTAHAPHEQALAEL
                     LRIFSALLDAGSATNEYVLHWNRNPEEFDFHFVRDGENVLLEIFQYPSDEREPENREK
                     VFAHTGNLKDVAEAFHKTFTQLYRDRDTDEFEFNWRQPFPLAEFEIFESTLKSARS"
     gene            7840..8685
                     /gene="thyA"
                     /locus_tag="VGB68_10855"
     CDS             7840..8685
                     /gene="thyA"
                     /locus_tag="VGB68_10855"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEY0049778.1"
                     /translation="MKQYQELLRYILDKGARHEDRTGVGTISVFGYQTRFDLRAGFPI
                     VTTKRVPFRWIFEELAWFLSGDTSEKNLRARGVDIWQEWATPEQTARFNREEGDLGPV
                     YGYLWRSFGGDYPQTNGVDQIERLIREIEKNPNSRRLIVTGWNPATCDEVALPPCHTL
                     FQFKIENEKTLHCQLYQRSADAFLGVPFNISSYALLTHLIAHVCDLQIGEFIHTFGDL
                     HIYSNHLEQVEELLKREPLPLPKLELKNAENLKGLKGLLNFRYENLQLEGYQSHGKIA
                     APVAV"
     gene            8700..9398
                     /locus_tag="VGB68_10860"
     CDS             8700..9398
                     /locus_tag="VGB68_10860"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HEY0049779.1"
                     /translation="MKKFLTAKWLDLVMANYRVEPALLEEFVPRGTSLDFWNGEAYVS
                     LVAFKFFDTRVLGFRIPFHTNFLEVNLRFYVKREIENETKRGVVFIKEIVPRRAIAYV
                     ARYAYGEPYEAWRMRHEQRENELVYLWLKGGRTNQIKVNFQNEIGVPLPDSHESFIIE
                     HYWGYTKRGAERTDEYKVEHPRWSLYNARDAEIAVDFGATYNEKFAFLTGENPKSVLM
                     AKGSEIAVYKGAKI"
     gene            9401..9883
                     /locus_tag="VGB68_10865"
     CDS             9401..9883
                     /locus_tag="VGB68_10865"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEY0049780.1"
                     /translation="MIIGIVAVAQNLAIGKDGKLPWHYSPDLKFFKQTTLDNAVVMGF
                     NTWQSIGKPLPNRLNIVLSRSNSIENQPSVLLLRSREQILALAKYLNCDLFIIGGAET
                     YKNFSGEIEKWIVTEIPETIENADAFMPPDFLDNFELKEIKELNRSLQAKTYLREYLM
                     "
     gene            complement(9960..10838)
                     /locus_tag="VGB68_10870"
     CDS             complement(9960..10838)
                     /locus_tag="VGB68_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY0049781.1"
                     /translation="MKKCQKCNYENADSMRFCLECGTPLADAPIIISWQDSETQKQSN
                     VDTASFGQSMETHVGGRPAFPNNLPIVSPASKPRGNKKLFIVIGGIAALFLLVFAAGA
                     AIIGYNLIYTPKPVVYNPTPTASPTRSAEKSPKPSPSKSASATPTASPEDSPEDSPKS
                     DSDAKTEFDRIWVDYNVTEKGRLGMRIHVDCSVKNMKDVSSYLALYFQKKDGTKLLTN
                     KQGFRSKDGQVALYKALKPAYDDAVYEDSQFFIPYEEFNLKRGKYDLQIDLDLIYENG
                     DLAEHMTFYDFEYEKK"
     gene            10978..>11202
                     /locus_tag="VGB68_10875"
     CDS             10978..>11202
                     /locus_tag="VGB68_10875"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEY0049782.1"
                     /translation="MPPRALAIDIGTSSVRAALYDERARVLPETMVKNERVLTATEDG
                     GAEIDADEAFRQVVEAIDAVLKKTKNLKGEI"
BASE COUNT         2869 a         2810 c         2667 g         2856 t
ORIGIN      
        1 ttcgcgaccg cgttaacgag aaaaataaag tcgaaggcac gaatgcgaaa ttctcgatag
       61 ttcaataaaa ttgctttgaa aattaagctt taagccgacg ctttccagaa agacgtcggc
      121 ttttttaatt atggaaaccg aaagactggt tttaaggcgt tacgctgaaa aagacaagcc
      181 ggattttatc gcgcttttta ctgacgcggc ggtgatgaaa tacgtcggcg acggcgtgtt
      241 gaccggagag caagccgaag ccttctggca gaagcttttt gaaaagctct acccgcaaaa
      301 tttcaatatc tgggcgattt ctgcaaagga agattcagca tatatcggac acgccggaat
      361 ttacccgcga ccgtcgcata aagaagattg ggaattcgtt tattttctca accagaaagc
      421 ctggggcaaa ggctacgcga cggaaatcgc ccggcggata atcagctttg cgtttgaaga
      481 cttaaattta ccggaagttt tcgccacggt tgacgacgcg cattcggctt cgattcacgt
      541 tctgaaaaaa gccggaatga gttttaagaa ttacgaatac gacgaaaagg gcagattttc
      601 ggtttattcg ctgagaaaac gaaatcgcgc agataccgaa gcgtaaagaa agattgaaac
      661 aggatttacg ggttgaaagg gataaattta ttttttttct gcgtttgtct gcggaaacgg
      721 gcgtttaatt ctttccaaaa tctttatgtc cgcgaggttt aaaacggtaa tttatttctt
      781 taaaaaatta tcaaaccaca attgaaaaac cagcagagtc cagagctgtt tgtgatgcga
      841 agccgcgccg gcttcgtgtt cggcgataag tttctgcacg taaccggcat caaataaacc
      901 ttgattttcc aatctttccg gcgcgagcaa atcgcgcatt aaaggattca ggcgaccttt
      961 cagccaatcg gcaatcggaa ttccgaaacc ttttttcgga cgctgcaaaa tcgttttcgg
     1021 cagcaaatct tcgaccgctt tttttagaat atatttacct ttgctgcctt tgagcttgta
     1081 ttcgagcggc aggctcgccg cgtattcggc aacgcgcgaa tcaagaaacg gcgcgcgaac
     1141 ttccagcgaa accgccatcg aagcccggtc gactttcgtc agaatatctt ccgccatata
     1201 gaaattcata tcgagaaact gcatctgttc gatttcgtcg gtggcgtcgg taatttttaa
     1261 taaatctttc gcccccttgt aaatatcgcc cgaagtgcct gccggaaagt cttttgccag
     1321 taaatcgttt tgcgcgtcaa tcgaaaacga gccgaaccac gaatgatgac gcgtaaccgc
     1381 atcgtatttc gaagccgcga caaaacgttt cgccttgtaa tcaaacgaaa gattttttgt
     1441 cgaaacgggc agattattga caatcggctc aatcaagccg gcgcgcaaaa agcgcggcag
     1501 cctgtcgtaa acgcgcgcca ttttatgtcc gaaatacatc gggtaaccgg caaaaatctc
     1561 gtcgccgccg tcgccgccga gcgcgaccgt cacgtgtttg cgaacaaagc gcgacagcag
     1621 aaaagtcgga atcaatgagc cgtccgacac gggctcgtca agccacgttc cgatttcaga
     1681 aatcaaatcc gccgcttttt cgacgctcaa tttttcttcg taatgttccg ttcccagatg
     1741 ttcggcaacc tgccgggcaa acttcgattc gtcaaacgaa tcttcctcga aaccgataga
     1801 aaatgttttg accttttccg ttgcgtgctg cgtcgcaaaa gcggcgaccg tcgaggaatc
     1861 gaccccgccc gaaagcaaaa ttccgagagg cacgtccgaa accaggcgca tccgaaccgc
     1921 gtcggaaata atttcgcgca gctctttcgc cgccgtttca accgtcggcg tattgccgtt
     1981 tttgtgaaag ctcaaattcc agtaacggcg cgttttcact tcgccgtttt cgacggttaa
     2041 aagatgagcg gcgggaagct tataaatatt tttgtaaatt gaaagcggtg ccggaacata
     2101 gtcgaaacaa agatattgac gaagcgcctc cggattgatt tcaggctcga ccgcgggatg
     2161 ccgcaaaaga actttcggct ccgaggcgaa aatcaatttg cccgcgaaaa cgccgtaata
     2221 aagcggtttt tcgccgaagc ggtcgcgggc aatgagcagt tttttgcggc gcaaatccca
     2281 cagagcgaaa gcaaacattc cgttcagatg ctcgaccatt gcttcgccgt attcttcgta
     2341 aagatgcggc aaaacttccg tgtccgaatg gctgtgaaat tcgtgaccgc gccgctccag
     2401 ctcttttctt aactcgcgaa aattataaat ctcgccgttc atcacgacga cgattgattt
     2461 atcttcgttc cagaaaggct gttcgcccgt ctgcaagtcg atgatggaca gccgccgcat
     2521 tccgagcgcg accgattcgc ccgtccacaa tccttccgag tcaggaccgc gatgcttcat
     2581 ccgctcgcac atcgcgtgca aaacggcttc gccgccttcg ctcgaagatt tatcgtctaa
     2641 atttgcccag cctgcaattc cgcacataaa aagtgaaaag ccgttattta ttatttgtca
     2701 tcaaacccga ttcgctcgcg cactgtataa atcgccttgt tctgcgattc gtgataagtc
     2761 ctgacgagca tttccgccag taagcccatt aaaaaaaact gcacggaaat ggcgagcatc
     2821 acaatcgcaa ttatcggcag cggcgtcaat atgaacgaaa cgccttcgac aaatttcaaa
     2881 aagaaagccc agatagcggc gactatcgaa agcagaaacg caatcatccc gaacgtgccg
     2941 aaaacgtaaa tcggtttcgt ctggtacgaa gccataaatt ttatcgtcat caaatcgaaa
     3001 acgactttga ttgttctgga aattccgtat ttcgatttgc ccattatgcg cgcgtgatgg
     3061 tcaaccggaa tctccgtgac gcgcgcgccc gcccagctcg cgtaaatcgg gataaagcgg
     3121 tgcatctcgc cgtaaagccg cacgtcctga attacctcgc ggcgataggc tttcaggctg
     3181 cagccgtaat cgtgaagcgg aacgccgcca atccacgaaa tgattttatt ggcgatttgc
     3241 gacgggattt tgcgcgaaat cagcttgtcc tgccggtttt ttcgccagcc ggagacgacg
     3301 tcgtaacctt cgtctaattt ttctaaaaga cgcgcgatgt ctttcgggtc gttttgcaaa
     3361 tcggcgtcca tcggaatcag aatttcgcct ttcgccgcgt cgattccggc gctcatcgcc
     3421 gccgtctgcc cataatttct gcgcagcgaa atgacgcgga ctttttcgtc ctgctcggcg
     3481 atttccctta aaatttggag acttttatcg gtcgagccgt cgtcaacgta aatcacttcc
     3541 gccgttttgc ccagcgaaag cagcgcttcg cgaatcttcg cgtgcatcgg gcgcagattt
     3601 tcttcctcgt tcaggacggg cagaaaaagc gacaattcgg gcatcgcgtc ggtttttggt
     3661 aaatcggtca gctctgtttg cactgaaatc attttcatct agaccgcaaa tgtaataatt
     3721 tactgctttc cgcctgaaaa atacagtttg tagaaagaga tttcacgatt tagaaacaga
     3781 gatgtaaaga agaactcaaa caggatagac aagttttggc aggcttcaag gcttgataaa
     3841 atttattttt ttcacgttat ccgtgtgaat ccgtagtttc atttctccgc cttaaactac
     3901 ccgcgcaacg ggcgattatg actttttcgc gacgattaca atcgaaacgc cgaagggaaa
     3961 atcgaagttt ttcagccaga agcgttccgc gccgaagagt ttgccgaaaa cgccgttcag
     4021 ggcggaaacg ttgacgttat tttccgattc gggcttgatt ccggttattt tcatcagcgt
     4081 tctgcccgcc agaatcggcg cgaaaaacgt ccagttggcg tatgtcgcgc gctcgatttc
     4141 aaaaccggct ttttcgagcc gctcgacaat ctgttttttc gtgtagcgaa tccggtgatt
     4201 ggaaatatcg tcctgcacgc cccagagcca cataaacgcc ggaacgaaaa tcagcgtgcg
     4261 cccgcccgat ttcaaaacgc ggtgcatttc ctttaacccg gcagcgtcgt cgtcgaggtg
     4321 ttcgacgacg tcgagagccg tcaccaaatc aaagctttcg tcgtcaaacg gcagcttttc
     4381 cgccaagcct ttgtgcgttt tcaagccttt ggcgcggcaa aactccagcg cgctgtcgga
     4441 aacatcgacg ccttccgccg cgccgaattg cgcgagcatt tccagattag cgcccgtccc
     4501 gcagccgacg tcgagaattt tcagtgccga gtcgcgagcg ccgagcgccg agtgaatttg
     4561 ccgcagaaag ctttccagaa tcaatcgccg cccgacaaac caccagtgcg aatcctcgac
     4621 gcggttcata atggcgtaag tatgctgctg catttcctgc ggtaaagccg agctcataaa
     4681 tttattttgc cataaaaagg cgtaaatagc gtaggctgga agattcctga ttcggaaatc
     4741 gaatttttaa tcctgagtcc tgaatcttaa atcccgaatc ctgcattatc tttgccgcag
     4801 cccgaacatt tgactgaccg agctgccgcg gttgccgtgt tcctcaagcc cgtagctttc
     4861 gcgcaggcgg cgcttgatgc catcgtcatc gacaaagcat ctgccgtcgc cgccgcgccc
     4921 gagaatcatt aaatccaatc cttcctgcaa atattcgtcg cggtcgattt gcagcatata
     4981 atcgcccgcc gttttatgcg ccagccattc cagctcgtaa agcgtcgggt catacaggct
     5041 caaaggattg acgtccgcat cggaaaagct cgcaatcgtc tgcgcgaaat cgaaagcgtg
     5101 gcgaccttcg tggacgaaag ttccctcgca gccgcgctgc ccgcccgtgt caatcgtttc
     5161 ttcggcaatc gtgataaaaa cttcgccgag cgcctcgcgc gggctcaata tttccgaatc
     5221 gattttatcg ttcgttttcc gcgcattgat gatgccggta atgcccgaag cattaacgcg
     5281 gctgaccgga aaaacgcaga ccagcatttc agattcgatg ataatttcca tcatctcgcg
     5341 atgaaaatcg ctgccttttt cgagaatcgt cgcaaaagcc tcttcgattg aacggcgata
     5401 ttttttggcg atgtctttgt cgaatctcat actttatatt ttttacaact tccgttttta
     5461 ttaagacttt taatcaccgt cttcaattgt cacgcttaaa cccgaattga ttaaagctac
     5521 tccaaaaaag ggttcggcgg ttttgttcgc tcttaaatct tccagcaagc tctgcgggtt
     5581 aataatttga ccgatctttt gttcggattc cggtctcggg ttatttgatt ctctggctcg
     5641 gtaaattcta atccgtttcc cgtcgtctat cgcctttcgg caaaccgccc gcgcgtaaaa
     5701 tttattaaat tcaccttctg caagtatctc aggcgcgttt ttccgcgtgt tgcgcagcaa
     5761 tgtttccccg tttctctgat aactttccgt cggattcagc aaatcctgat ttctgatttg
     5821 ctcggttaac cagacttcat cgtgtcttag aatcgcctcg gtcagcaatt gctgccacag
     5881 gctgcgcccg cgcgagctca agcccttact caaatacaaa gcgttgtttt caaggtcgaa
     5941 tttgatttct tcgctcatca aatctctaaa gtcgtcaagg tcgataaaat taaatgacat
     6001 atgtgcctcc gataaatttc ctgccctcta aattatcttt ccgaaaccgc accaggaatt
     6061 ttgattatgc cagataatct cgacatcccg cgcgttaatt tcctgccacc aacgggtaaa
     6121 ttcgtccctg gaaatataaa atgccgtcgg cgcgaccaga tggtcgaaga cgatgttgtg
     6181 ctgctcgcgc cagccgaatg tgcctagatg agacaggtat tcgtgataga aaagcttttt
     6241 gccgaacgac gttttgtcca gcggtttata aattagtttg ctggctaaaa aaacgccgag
     6301 cgtcggcagc ttcgataatt gataaagcgc cggctggctg attttcgacg tgaagccctc
     6361 gcgaatcggg ttgacgtagc gcgtaatcca ttcgttgttt tccgcgccgt aaatccaagc
     6421 cgaaatgtgc ccgccgtttt ttactttcga ggcgagcgcc agaaatgctc ctttcgggtc
     6481 gggcgtgtga tgcagaacgc cgaccgagaa agcgtaatcg aaagcgcgtt tgaaaggaag
     6541 cttgaaaata tccgcctgaa cgatgtgcgc gttcggcaat gcgcgcgtcg cctgaaacgc
     6601 cggttcgacc gccgcgctca aatcaatgcc gacgacttcg cgcgcgcccc aatttgccgc
     6661 caaactcgta tgacgacctt tcccgcagcc gccttccaga acgaatttat ctttaaaaaa
     6721 atcttctttg accggctgca gccagcctaa aaattgctcg gcgtattttt cgtcagcctg
     6781 tgtaaaatgc gtccattgcc agccgaaatt ttcggcggtg tcggctttgt cctgctcgat
     6841 tttcgataaa tcggcgaaac gcggaacgcc gcgcgcgatt ttgtattcgc gcaggcattt
     6901 tttacacgac aaaacgcctt cgatgatttc cctgtcctcg tatttgcccg cgtatgagag
     6961 cagaatgtcg ccgccgcagg tcgggcaggc taaaaggtct aaaagttttt ctttcattat
     7021 tatgcagcca cgaaaaaaca cgaaagaaca cgaaaaataa tttaaaaatt tcgtgtattt
     7081 tcgtgtctgt tcgtggctaa atcgtattct tattcaccga gttgtgcaag gaacgccggg
     7141 actggcgcag ccgctcgtca atttcccgcc atcccgcgag caaaacgccg gcaaccggca
     7201 aagcgagaaa aattccgaac aaaatccttt tcttatcaaa gattaacttc gtgcggattt
     7261 cagagtggat tcaaaaattt caaattcggc gagcggaaac ggctgtcgcc agttaaactc
     7321 gaattcgtcc gtgtcgcggt cgcggtaaag ctgtgtgaaa gttttatgaa acgcctcggc
     7381 gacatctttc aaatttcccg tatgagcgaa aactttctcg cggttttccg gctcgcgctc
     7441 gtcgctcgga tattgaaaaa tttccagtaa aacgttttcg ccgtctcgca caaaatgaaa
     7501 atcgaattct tccgggttgc ggttccaatg caaaacgtat tcgttcgttg ccgaaccggc
     7561 gtccagcaaa gccgagaaaa tccgcaaaag ctccgccagc gcttgctcgt gcggcgcgtg
     7621 agcggtcgtc gtgtgaaact cggatgagcc gtcgtcgaaa ccgatcgaca tccacccgca
     7681 ctgcgggctg ttgaagctga cttccagttt atttgccata aattcggtgt gaaacgtaag
     7741 tcgcaaatcg tgaaaccaac cttctgcgat tcacgattta cgatttacgt tttgcggctt
     7801 taatatggta actttaagca gaaatttgca caaattcgga tgaaacaata tcaggaactt
     7861 ttacggtata ttttagacaa aggcgcgcgc cacgaagacc gcaccggcgt cggcacgatt
     7921 tcggttttcg gttatcaaac gcgtttcgat ttgcgcgcgg gctttccgat tgtaacgacg
     7981 aaacgcgtgc cgtttcgctg gatatttgaa gagttagcct ggtttttaag cggcgatacg
     8041 agcgagaaaa atctgcgcgc gcgcggcgtc gacatctggc aggaatgggc gacgccggaa
     8101 caaaccgcgc gctttaatcg agaagagggc gatttaggtc cggtttacgg ctacctgtgg
     8161 cgaagcttcg gcggcgatta cccgcagacg aacggcgttg accagattga aagactgatt
     8221 cgcgaaattg aaaaaaatcc taattcgcgg cgtctgattg tcaccggctg gaatccggcg
     8281 acttgcgacg aagtggcttt gccgccgtgt cacactttgt ttcaattcaa aatcgaaaac
     8341 gagaaaacgc tccattgcca gctttaccag cgcagcgccg acgcttttct gggcgttccg
     8401 tttaatatca gctcttacgc gcttttgacg catttaatcg cgcacgtctg cgatttacaa
     8461 atcggcgaat tcatacacac tttcggcgat ttacacattt attcaaatca tctggagcag
     8521 gtcgaagagc ttttaaagcg cgagccgctg ccgctgccga aactggaatt gaaaaacgcg
     8581 gaaaatttaa aaggcttgaa aggtttatta aatttcagat acgaaaatct tcagctcgaa
     8641 ggctatcaat cgcacggcaa aatcgccgcg cccgtcgcag tctaagtcag aatcgagtta
     8701 tgaaaaaatt cttaaccgca aaatggctcg atctggtgat ggcgaattac cgggtcgagc
     8761 cggcgctgct cgaagaattt gtcccgcggg gaaccagtct cgatttctgg aacggcgagg
     8821 cttacgtcag cctcgtcgct tttaaatttt tcgacacgcg cgtgctcggt ttcaggattc
     8881 cgtttcacac gaattttctg gaagtcaatt tacggttcta cgtcaaaaga gaaatcgaaa
     8941 acgaaacgaa acgcggcgtg gtttttatca aggaaatcgt gccgcgccgg gcgattgcct
     9001 atgtggcgcg atacgcttac ggcgagcctt acgaagcctg gcggatgcgt cacgagcagc
     9061 gcgaaaatga gctggtttat ctctggctga aaggcggcag aaccaatcaa ataaaagtta
     9121 attttcaaaa cgaaatcggc gttcctttgc cggattcgca cgaatctttt atcatcgagc
     9181 attattgggg ctacacaaaa cgcggcgctg aaaggacgga cgaatacaag gtcgagcatc
     9241 cgagatggag cttgtataat gcgcgcgatg cggaaatcgc ggttgatttc ggcgcgactt
     9301 acaacgaaaa attcgctttt ttaaccggcg aaaatccgaa atcggtttta atggcgaaag
     9361 gctcggaaat cgccgtctat aaaggcgcaa aaatataaaa atgattattg gaatcgtcgc
     9421 cgtcgcgcaa aatctcgcca tcggcaaaga cggaaaactg ccgtggcatt attcgccgga
     9481 tttgaaattt ttcaaacaga cgacgctcga taacgccgtc gtgatgggct ttaacacgtg
     9541 gcaatcaatc ggcaagccct tgccgaatcg gctgaatatc gttttaagtc gctcgaattc
     9601 gatagaaaat cagccgtcgg ttttgctttt gcgaagccgc gaacaaattt tggcgctggc
     9661 aaaatattta aactgcgatt tgttcatcat cggcggcgcg gaaacttata aaaacttttc
     9721 cggcgaaatc gaaaaatgga ttgttacgga gattcccgaa acaatcgaaa acgccgatgc
     9781 ttttatgccg ccggattttt tggataattt tgagttaaag gaaataaaag agcttaaccg
     9841 cagcctgcaa gcgaaaactt atttgagaga gtatttaatg taaagcaaaa gtaaaaagga
     9901 aaaggcaaaa gtgcgaaatc aaatgccttt tgccctttta ccttttgctt taagctctct
     9961 tattttttct cgtattcaaa atcgtaaaaa gtcatatgtt cggcgaggtc gccgttttcg
    10021 taaatcaaat ccaaatctat ctgtaaatca tatttgccgc gtttcagatt aaattcttca
    10081 tacggtataa aaaactgcga atcttcatac acggcgtcgt cgtaagccgg tttcagagcc
    10141 ttgtaaagcg cgacctgccc gtctttgctg cgaaagccct gtttgtttgt caaaagcttc
    10201 gtgccgtcct ttttttgaaa atacagagcc agataagaac tgacgtcttt catatttttc
    10261 accgagcaat cgacgtgaat ccgcattccg aggcgacctt tttcagttac gttgtaatcg
    10321 acccaaatcc ggtcaaactc ggttttggcg tcggaatcgg atttcggcga atcttccggc
    10381 gaatcttccg gcgaagcggt cggcgtcgcg gaagccgatt tggacggcga aggtttcggc
    10441 gatttttcgg ctgaacgcgt cggcgaagcg gtcggcgtcg gattataaac gaccggtttc
    10501 ggcgtataaa tcaggttgta gccgataatt gccgcgccgg cggcgaaaac cagcaaaaac
    10561 aaagcggcga tgccgccgat aacgataaac aatttcttgt tgccgcgcgg ttttgacgca
    10621 ggagacacaa tcggtaaatt attcggaaaa gccggtcgcc cgccgacgtg cgtttccata
    10681 ctttgaccga aagaagccgt atcgacgttc gactgttttt gcgtttcgct gtcctgccag
    10741 ctaatgataa tcggcgcgtc cgcgagcggc gtgccgcatt ccagacaaaa ccgcatcgag
    10801 tcggcatttt cataattaca tttctgacac tttttcatcg gtaaaaaccc cttcagcaaa
    10861 gacagatttt gatgataatt ttgcggtcgg gctgaataac gtcgataata aagccaaaac
    10921 cgtaaaattc aaagccattt tctttagcgc cgattttcga ttaaacttaa gcttcaaatg
    10981 ccgccacgag ctttagccat cgatatcgga acttccagcg tccgcgccgc gctttacgat
    11041 gaaagggcgc gcgttttgcc cgaaacgatg gtaaaaaacg agagagtttt gactgccacc
    11101 gaagacggcg gcgcggaaat cgacgcggac gaagccttca ggcaggtcgt cgaagcgatc
    11161 gacgcggttt tgaaaaaaac gaaaaactta aaaggcgaaa tc
//
LOCUS       DASWNE010000546         2302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_476761, whole genome shotgun sequence.
ACCESSION   DASWNE010000546 DASWNE010000000
VERSION     DASWNE010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476761"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VGB68_10880"
     CDS             complement(<1..441)
                     /locus_tag="VGB68_10880"
                     /inference="COORDINATES: protein motif:HMM:NF025946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY0049783.1"
                     /translation="MKNYIEKSMTFAEYIKLIDDLLLDGKTTGTNQSEAMFNYGKLNR
                     QRMHRLEKTVALIDSVKEKARNVERRMIWLIITEGWCGDAAQNIPVIEKIAAQTPNVE
                     TRYVLRDENLELIDAHLTNDARSIPKLVALDAATFKVLGTWGPRA"
     gene            complement(448..1071)
                     /locus_tag="VGB68_10885"
     CDS             complement(448..1071)
                     /locus_tag="VGB68_10885"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY0049784.1"
                     /translation="MSNKFYDIAFTPDVKQAQRRYGSRRRYEKIENSEREQNAIFSDA
                     EIDFIGQRDGFYMATVGDGGQSYIQFRGGRRGFLKVLDEKTLGFADFRGNLQYISAGN
                     LSGNKKVALFLMDYANRQRLKILAEAEVKDAAEAPELIEKLRDESYEAKVEQAMILHV
                     KAFDWNCPQHITPRYTVEEVKAIIQPLNEYVQKLEKEIEKLKAKTPD"
     gene            complement(1109..1258)
                     /locus_tag="VGB68_10890"
     CDS             complement(1109..1258)
                     /locus_tag="VGB68_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049785.1"
                     /translation="MNLCAAARIEKECLLSRKPSTILLTLSSELLVVYPWFLRLSLFL
                     SRLMQ"
     gene            complement(1290..1766)
                     /locus_tag="VGB68_10895"
     CDS             complement(1290..1766)
                     /locus_tag="VGB68_10895"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY0049786.1"
                     /translation="MSSNSRFAIAVHTLALMANHEDSPLKSEFIACFVKTNPVVIRRL
                     LPELAKADLVETQAGAHGGSRLKKKPEEISLWEIYQAVESSAAFSVHQPLSEGECKIS
                     RNIESVLINIQNRVDKAVWQTLRCITLAEILRLIREEKDDLLGERCGETKNDAERV"
BASE COUNT          596 a          562 c          468 g          676 t
ORIGIN      
        1 cgcgcgaggt ccccaggtgc caagcacttt gaacgttgcg gcgtcgagag cgacgagttt
       61 cggaatcgag cgcgcgtcgt tcgtcaaatg cgcgtcaatc aattccaaat tttcatcgcg
      121 caaaacgtag cgcgtttcca cattcggcgt ttgcgcggcg attttttcga taaccggaat
      181 attctgcgcc gcgtcgccgc accagccttc ggtgataatc agccaaatca ttctgcgctc
      241 gacatttcgc gctttttctt tgaccgaatc aatcagcgcc accgtttttt ccagacgatg
      301 catccgctgg cgatttaatt ttccgtaatt aaacatcgct tccgattgat tcgttccggt
      361 cgttttgccg tctaaaagca aatcgtcgat gagcttgatg tattcggcaa aagtcattga
      421 tttttcgata taatttttca tattcgttca atccggcgtt ttcgctttta atttttcaat
      481 ttccttttcc agcttctgaa cgtattcatt caaaggctga atgattgcct tgacttcttc
      541 aacggtgtag cgcggcgtga tatgctgcgg gcagttccag tcaaacgctt tgacgtgcag
      601 aatcatcgcc tgttcgactt tcgcttcgta actctcatca cgcagttttt caatcaattc
      661 cggcgcttcc gccgcgtctt tcacttcggc ttccgccaga atttttaggc gctgccggtt
      721 tgcgtaatcc atcagaaaca gcgcgacttt tttgttgccg cttaaatttc cggcgctgat
      781 gtattgcaga ttgccccgaa aatcggcaaa gccgagcgtt ttttcgtcca gcactttcaa
      841 aaatccgcgc cgaccgccgc gaaattgtat atacgactga ccgccgtcgc cgacggttgc
      901 catataaaaa ccgtcccttt gcccgataaa atcaatctct gcgtcggaga aaatcgcgtt
      961 ttgctcacgc tccgaatttt caatcttctc gtagcgccgc cgcgagccgt atcgccgctg
     1021 cgcctgtttt acgtccggcg taaaagctat gtcataaaat ttattcgaca tttcaaaaaa
     1081 aaatatttcc caagccggtt caatggtttt actgcatcag tctcgacaaa aaaagactca
     1141 atctcaaaaa ccacggatag acgacgagca attccgaaga caaagtcagc agaatcgtcg
     1201 acggcttccg gctcaaaaga cattcttttt caattctcgc tgctgcacac aaattcataa
     1261 aaaaacttcc ccggctcgtg taatttttgt taaacccgtt cggcatcatt cttcgtctcg
     1321 ccgcatcgct cgccgagcaa atcgtctttt tcttctctga tcaaccgcaa aatctcggct
     1381 aaagttatac agcgcaaagt ctgccagacg gctttgtcga cgcggttttg aatattgatt
     1441 aaaacgcttt cgatattgcg gctgattttg cattcgcctt ccgacaaagg ctggtgaacg
     1501 gaaaaagccg cgctcgattc gaccgcctga taaatttccc agagcgaaat ttcttccggc
     1561 ttttttttca gacggctgcc gccgtgcgcg cccgcctgcg tttccactaa atcggctttg
     1621 gcaagctcgg gcaaaagccg ccggatgacg accggattcg tcttgacaaa acacgcgata
     1681 aactccgact tcagcggcga atcttcgtga ttcgccatca gagccaaagt gtgtaccgcg
     1741 attgcaaaac gactgttact tgacatttct ttattcgtaa caaatgatgt tacagtaaca
     1801 actatagtta cagatgcccg ttttgtcaag aggaaatttt cagtgaataa aaaaaatatc
     1861 ggctttgcac aaaaataaag aaagggaaaa ttgtaaattg taaattgtaa attctaaatt
     1921 gatgttcggt atcttttgtg aagtaattta gaattaataa ttgagaattt acaattagcc
     1981 atcggaattt ttctcgattt cctttatttt tgtgcaaagc aaaaaatatc ctcgtaaagt
     2041 aaatcaatta atttcaatct ctttttccgg cgcgccaaaa tttccgcgct gtttgtttta
     2101 aaatcaaaca ctttcggcag gcgttagatg aaaattcttt gatttcccag ttggcacaat
     2161 gattgcttaa actctgtttg aaatcgcgga gtgtatcaaa atcattccaa taaattatcg
     2221 aactaaggga aggtttctta tgaacgcaat tcaattattg aaagacgacc acagaaaagt
     2281 cgagcggctt tttcagaagg ta
//
LOCUS       DASWNE010000547         3738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_614920, whole genome shotgun sequence.
ACCESSION   DASWNE010000547 DASWNE010000000
VERSION     DASWNE010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614920"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VGB68_10900"
     CDS             <1..804
                     /locus_tag="VGB68_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049787.1"
                     /translation="DLYWGPGGQQMQPVLEKSKYVGRQAGGNNPKHRLKDGAGRDWVA
                     KMADESQPETAAVRLLWGIGYRTEINYLVPKLEIAKVGNYKNVRFEARPEHIKRLERW
                     SWNDNPFAGTNEFEGLKIMMAMFNNWDLKDENNIILQDGDRHYYVISDLGSSFGRLAE
                     TSVSRSGRTVNKPEDYAQSNFIRQVRDGIIEFDYRGSADYLVKGIKVEHGRWLADLLM
                     QLSDKQIEDAFRAANYEPEDVKLLAQAFKARINKLDAATKSPANTAKYE"
     gene            1732..3030
                     /locus_tag="VGB68_10905"
     CDS             1732..3030
                     /locus_tag="VGB68_10905"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY0049788.1"
                     /translation="MTVYHSKYFAYELTKQSASDSIAKLGASLFNAAVDLNPHQLDAA
                     LFAFRSPLSRGSILADEVGLGKTIEAGIIISQLWAERKRRILVICPTILRKQWAQELA
                     EKFFISSRIVDSREYNQYKRAGADNPLFKDNHVTICSYHFAYAKETELAATGWDLVVI
                     DEAHRLRNVYKSSNRIAAGIKRAVKDRATLLLTATPLQNSLLELYGLVSFLDERLFGD
                     INAFRARYMRGTVEERELRERRQRLKPIVQRTLRRQVTEYVRFTNRIPITQDFTPTAD
                     EQKLYEQVSGYLRREKLNALPGSQRKLMTLVLRKLLASSTFAISGTLRSLAARLQYPA
                     REVASEKPPATEPGEDFEIYDEVAEEWSDDEIDGNRQARASDVRENDEIADIQAELAE
                     IVEYSKLAASITTNAKGEALLQAPAWAFKSWKNWARREKP"
     gene            3145..3705
                     /locus_tag="VGB68_10910"
     CDS             3145..3705
                     /locus_tag="VGB68_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049789.1"
                     /translation="MSVSRLQITSLEEEDRLLYAVITDEGETLHPETGEKFFRVRGSI
                     SQNIEPRAETGGALQKRSRVLREQAVAEINARNSKFFDEEIEKLERWSDDLKYGLEVE
                     LKELDARIRALKKDAGLALDLQGKLEMQRQLKDAEAERSRKRRRLYEAQDRIDEQKED
                     LISSVEARLRQKIETEDVFTIRWKLI"
BASE COUNT         1001 a          904 c          970 g          863 t
ORIGIN      
        1 gatctctact ggggtcccgg cggtcagcag atgcagcccg tgctggaaaa atccaagtat
       61 gtcgggcggc aagcgggcgg caacaatccg aagcaccggc tcaaggacgg cgccggtcgc
      121 gattgggtcg ccaaaatggc agacgaatca cagccggaaa cggcggcggt tcgcctgctt
      181 tggggaatcg gttacagaac ggagattaac tatcttgtgc cgaagctcga aatcgcaaaa
      241 gtgggaaatt acaaaaacgt gcgctttgag gcgcgtcccg aacacatcaa gcggctggag
      301 cgctggagct ggaacgacaa tcctttcgcc ggcacaaacg aatttgaagg cttgaagata
      361 atgatggcga tgtttaacaa ctgggatttg aaagacgaga acaacattat tcttcaggac
      421 ggcgaccgtc attattacgt catcagcgat ttgggctcgt cgttcggcag gctcgctgaa
      481 acgagcgtta gccgttcggg gcgaaccgtt aataaacctg aggattacgc tcaatcgaat
      541 ttcatcaggc aggttcgcga cggaattatc gaattcgatt atcgcggctc ggctgattat
      601 ctcgtcaaag gaatcaaagt cgagcacgga cgctggctcg ccgacctgct gatgcagctt
      661 agcgacaagc agattgaaga cgcgtttcgc gcggcaaact atgagcccga agacgtaaag
      721 ctgctggcgc aagccttcaa agcgcgcatc aataaacttg acgcggcgac caaatcgcca
      781 gcgaacacag caaaatacga ataaataaag ggctcgaaat tcaaacgacg gcggaggaaa
      841 acggcggcgg caatctaact tcgctaagcc gccttacttc cgcctcgcgc tctatttttg
      901 agatagattt attcgttacc aatcttaccg gaaaatgatt cctaatcctc aatcgactgt
      961 aataatttaa tgcagcccgg cttgactcga aaatattaaa cggcgctttt gccgcagagc
     1021 ataagctcgt caaaataaat aatttaactc ttttaattaa gtttaggaac gccatttgca
     1081 aaggcttttg cgtgttattc tctccccaat gaattttaaa aacgggcagt tgacgtattt
     1141 attaaagctg caggctggat taatcggagc gggaaatgtt tgctagcttg ttttccgaaa
     1201 aaacgagctt tgaagtgatg ctttgcacaa aaataaagga aatcgagaaa aattccgatg
     1261 gctaattgta aattctaatt attaattctc aattacttca caaaagatac cgaacatcaa
     1321 tttagaattt acaatttaga atttacaatt ttccctttct ttatttttgt gcaaagcctt
     1381 gaagtgaata aaagtaattt tgaaaaagcc gtttcggcgg acgccgattt gcctgaagat
     1441 gttcggcgtc aaatagaatc cgccgtcaaa atttaccggg cgcgacgctc gacgttgcgg
     1501 gctggatttt aatcttgtat tttataaaag cctcgcgctc ggtaaatttc ttaaaagtat
     1561 tatcttatga ccgccctctg gaaacatctg catcgagact gccgctcgaa agttttatgg
     1621 cggggcgaaa taccgaatcg aaaagtcgac gttttcgctc gctgagattg gtttgcagtt
     1681 aatctcgcgg tttgttctaa ttttttatta cgcattgaca ctggtttcgg catgaccgtt
     1741 tatcactcga aatatttcgc ttacgagcta actaaacaaa gtgcttccga cagcatcgcg
     1801 aaactcggcg cgtcgctttt caacgccgcg gtcgatctta atccgcatca actggacgcg
     1861 gcgcttttcg cctttcgctc gccgctgtcg cgcggctcga ttctcgccga cgaagtcgga
     1921 ttgggaaaaa ccatcgaagc cggaattatc atcagccagc tttgggcgga gcgcaaacgc
     1981 cgcattctgg taatttgccc gactattttg cgcaagcaat gggcgcagga gctcgccgaa
     2041 aaatttttca tatcgtcccg gattgtcgat tcgcgtgaat acaaccaata caaacgcgcc
     2101 ggagcagaca atccgctttt taaggacaat cacgtgacaa tttgttctta tcatttcgcc
     2161 tatgctaaag agacggagct tgccgcgacc ggctgggatt tggtcgttat cgacgaagcg
     2221 catcgtttgc gaaacgttta taaatcaagc aaccgcatcg ccgccggcat aaagcgcgcc
     2281 gtcaaggaca gggcgacact gcttttaacc gcgacgccgc tgcaaaacag tcttttagag
     2341 ctttacggct tggtcagttt tttagacgaa cgccttttcg gcgacattaa tgcatttcgg
     2401 gcgcgttata tgcgcggaac ggtcgaggag cgggaattgc gcgagcggcg gcagcgtttg
     2461 aaaccaatcg ttcagcgaac gctgcgccgg caggttaccg aatacgtccg ttttaccaac
     2521 cggattccga ttacacagga tttcacgccg accgccgatg aacaaaagct ttacgagcag
     2581 gtctccggct atctgcggcg cgaaaaactg aatgctttgc cgggcagcca gcgcaaattg
     2641 atgacgctcg ttttgcgtaa actgcttgct tcgtcaactt tcgctatcag cggaactttg
     2701 cgctcgctcg ccgcgcggct tcagtatccg gcgcgcgagg tcgcgagcga gaagcctccg
     2761 gcaacggagc cgggtgaaga ctttgaaatc tacgacgaag tagccgaaga atggtcggat
     2821 gatgaaattg acggcaaccg gcaggcgagg gcgagcgatg tccgggaaaa cgatgaaatc
     2881 gccgacattc aagccgagct tgccgaaatt gtcgaataca gcaaactcgc cgcttcgatt
     2941 acgacgaacg ccaagggcga agcattgctt caggcgccag cctgggcttt caaaagctgg
     3001 aagaactggg cgcggcgcga aaagccgtaa tttttaccga atccagaaga acgcaggctt
     3061 atctctacga gcttctgacc gaaaacgggc acgccggacg agttttgacc attaacggca
     3121 cgaacggtcg ggcgttccgg ctggatgagc gtttcccgtc tgcaaattac ttcgctcgaa
     3181 gaagaagacc gtctgcttta tgccgttata acggatgaag gcgaaactct gcatcctgaa
     3241 acaggcgaaa aattctttcg cgtgcgcggc tcgatttctc aaaacataga gccgcgcgcc
     3301 gaaaccggcg gcgctttaca aaagcggagc cgggttttgc gagagcaagc cgtcgccgaa
     3361 ataaacgccc gcaacagtaa atttttcgac gaggaaatcg aaaagctcga acgctggtcg
     3421 gatgatttga aatacggctt ggaggtggag cttaaagagc tggacgcccg gattcgggct
     3481 ttgaaaaaag acgccgggct cgctctcgat ttgcagggca aactcgaaat gcagcgtcaa
     3541 ttaaaagacg ccgaagccga gcgctcgcgc aagcgccgcc ggctttacga ggcgcaggac
     3601 cggatagatg aacaaaagga agatttgatt tcttcggtcg aagcgcggct cagacaaaaa
     3661 atcgaaacgg aagatgtatt tacaatcagg tggaaactga tttaaatcgt ccggctgacg
     3721 gaacattacg gattttat
//
LOCUS       DASWNE010000548         2009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_88220, whole genome shotgun sequence.
ACCESSION   DASWNE010000548 DASWNE010000000
VERSION     DASWNE010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88220"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..410
                     /locus_tag="VGB68_10915"
     CDS             174..410
                     /locus_tag="VGB68_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049790.1"
                     /translation="MTSEKNKEAEAQAARTESEALSELNEPRWSVVSFESVAVHGLPY
                     AEAQNWLEKLQKQNISGLCIVTDEAAARISGQKI"
     gene            complement(433..1419)
                     /locus_tag="VGB68_10920"
     CDS             complement(433..1419)
                     /locus_tag="VGB68_10920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEY0049791.1"
                     /translation="MKAVYVREFGGIENLEIREVENPPKPAGKEVLVNVKASALNRAD
                     LLQRRGFYPAPAGFPERILGLEFAGEVAGIGENVSDFKIGDRVFGITAGGAQAEFLLT
                     DESLSARIPENLSFAEAAAVPEAFITAHDAVFTLGELKMDETLLIHAVGSGVGLAALQ
                     LAKARNIKTIGTSRTSGKLEKCGKFGLDEAIPAASETNFAQIIREKNQGKGVDVILDL
                     VGAKYFQANLESLNLKGRLILVGTTGGAKAELNYGLVMSKRIKIVGTVLRPRPTTEKA
                     EATRKFAEEVVPLLANGRIKPNVDKVFKIEQIREAHEYLESNASFGKVVLEF"
     gene            complement(1591..>2009)
                     /locus_tag="VGB68_10925"
     CDS             complement(1591..>2009)
                     /locus_tag="VGB68_10925"
                     /inference="COORDINATES: protein motif:HMM:NF037012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049792.1"
                     /translation="GTGIRHSEFNPSETESTHLYQIWILPEKDGLTPGYEQTYFAPEE
                     KKGKLRLVASRAGADGSVKINQDVSLYGSILASGEEVAHDLAENRHGWIQVVKGKLEI
                     NGETLNASDGAAISDERALKIKALENDTEFLLFDLN"
BASE COUNT          491 a          521 c          472 g          525 t
ORIGIN      
        1 acccggaaag ccggtcaaaa aaattgtttg cttgaaattc attttgttaa cgaaattata
       61 acttaaacgt ggtaatgatg taacgagccg gcgtttttat aaaaatgcaa ctctgttaaa
      121 acttttacaa tcaaagggat attatgttaa agtcaggcgc aaagatgaca aaaatgacat
      181 ctgaaaagaa caaagaagcc gaagcgcaag ccgcccggac ggaaagtgag gctctgagcg
      241 agctgaacga accgcgctgg tcggttgtga gtttcgaatc ggtcgccgtt cacggtttgc
      301 cgtacgcgga agcgcaaaat tggctggaga aactgcaaaa acagaatatc tccggcttat
      361 gtatcgtcac ggacgaagcc gccgctcgaa tctcgggaca gaaaatttag cgtcgaacaa
      421 atttgctcga cgctaaaatt ccaaaacgac tttgccgaag ctcgcgtttg attccagata
      481 ttcgtgcgct tcgcgaattt gctcaatctt aaaaacttta tcgacgttcg gtttaatcct
      541 gccgtttgcc agcagcggca cgacttcttc ggcaaatttt cgcgtcgctt cggctttttc
      601 cgtcgtcggg cgcgggcgca gaacggttcc gacgattttt atgcgtttcg acatcactaa
      661 accgtaattc aattcggctt tcgcgccgcc ggtcgtaccg actaaaatga gcctgccctt
      721 taaattcaag ctttccagat ttgcctgaaa atattttgcg ccgaccaaat ccaatattac
      781 gtctacgcct ttgccttgat ttttttccct gatgatttgc gcaaagtttg tttcgcttgc
      841 cgccggaatc gcttcgtcaa gcccgaattt tccgcatttt tcaagtttgc cggatgtccg
      901 cgaagttccg attgttttaa tatttcgcgc ttttgcgagc tgcagcgccg ccagcccgac
      961 gcccgagccg acggcgtgaa tcaaaagcgt ttcgtccatt ttcagctcgc ctaatgtaaa
     1021 aacagcatcg tgcgcggtga taaacgcttc gggaacggcg gcggcttcgg cgaaacttaa
     1081 gttttccgga attcgcgccg agagactttc atccgtaagt aaaaattcgg cttgcgcgcc
     1141 gcccgccgtg atgccgaaaa ctctgtcgcc gattttgaaa tcagacacgt tttcgccgat
     1201 tccggcgact tcgcccgcaa actccaagcc taaaatcctt tccggaaaac cagccggcgc
     1261 gggataaaaa ccgcgacgct gtaaaaggtc ggcgcgattt aaagccgatg ctttaacatt
     1321 aaccagaacc tctttgccgg caggcttcgg cggattttcg acctcacgga tttcgagatt
     1381 ttcgattccg ccgaattctc tgacgtaaac tgctttcata aaatcattgt agaaaaaata
     1441 aacagggatg gacagaatcg gcagaataaa aaaagcgtcc ggtaattgat aatggacgct
     1501 tcgaaattct catcaaattc tcctgcccgg tcaatcaagc cgcgaaaccc gctttatccc
     1561 tgtttgaatt gccgatgaat ttctctctaa ttaatttaaa tcgaaaagca gaaactcggt
     1621 atcgttttcc agagctttga ttttcagcgc tcgctcgtcg ctgattgccg cgccgtcgct
     1681 ggcgtttaaa gtctcgccgt tgatttccag ttttcccttc acgacctgaa tccagccgtg
     1741 acgattttcc gccagatcgt gcgcgacttc ctcgcccgac gccagaatcg agccgtagag
     1801 cgaaacgtcc tgattgattt tcaccgagcc gtccgcgccg gcgcgcgagg cgaccaggcg
     1861 gagctttcct tttttctctt cgggcgcgaa atacgtctgc tcgtaaccgg gcgtcaagcc
     1921 gtccttttcc ggcagaatcc agatttgata aagatgcgtg ctctcggttt cggacgggtt
     1981 aaattcgctg tgacgaattc cggttccgg
//
LOCUS       DASWNE010000549         1642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_688813, whole genome shotgun sequence.
ACCESSION   DASWNE010000549 DASWNE010000000
VERSION     DASWNE010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688813"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VGB68_10930"
     CDS             <1..552
                     /locus_tag="VGB68_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049793.1"
                     /translation="KKNTKSLTANAFVVAESRKSPALKRPAEETFGIWNEFGSENLGF
                     TIVFPAKSEEIRETGQGKIAGFETETEKAHYGVLRMKFPPLLDRRRLGILRESLFARG
                     YDAKTTKLKSEKNICLDGMAGKELIYEESGKIFFTRFYIMEQDMFMLSVSLPETQYTV
                     DFDYWAMKFLDSFGARTVGRHIG"
     gene            577..1383
                     /locus_tag="VGB68_10935"
     CDS             577..1383
                     /locus_tag="VGB68_10935"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEY0049794.1"
                     /translation="MIFVDSHCHIDGKEFDADREEVLRRAEEAGVRAMLVVGTGEASD
                     FENFERAVRVAESRENIFASIGVHPHDAKSFDPAAEKRLIDLAKSSKKVVAWGEIGLD
                     FYYDHSPRPLQAQVFRRQIHIARDLNLPIIIHSRDADEETVRILTEECGSEDFRGGIM
                     HCFGGSAAMAEDLMKIGFLISFAGNVTFKKAGNLRDAARAVPLERLLIETDCPFLTPV
                     PFRGRRNEPMYVVETARFLADFYGIELERLAERTTLNFTEFFGLTLKNGG"
BASE COUNT          417 a          392 c          460 g          373 t
ORIGIN      
        1 aaaaaaaata cgaaatcttt gaccgctaac gcgttcgtcg tcgccgaaag ccgcaaatcg
       61 cccgccctga aacgaccggc ggaggaaact ttcgggattt ggaacgaatt cggttcggaa
      121 aacctcggat ttacgattgt ctttccggca aaatccgaag aaatacggga aaccggacaa
      181 ggcaaaatcg ccggcttcga aacggaaacc gaaaaagcgc attacggcgt gctgcgaatg
      241 aagtttccgc cgctgctcga ccgccgccgg cttggcattc tgcgcgagag cctgttcgcg
      301 cggggctacg acgcgaaaac gacgaagtta aaaagcgaaa aaaacatctg tctcgacgga
      361 atggcgggca aagagcttat ctacgaagaa tcgggaaaaa tatttttcac cagattttat
      421 attatggagc aggatatgtt tatgctgagc gtcagcctgc ccgaaacgca atacactgta
      481 gatttcgatt actgggcgat gaagtttctc gattcgttcg gagccagaac cgtcggcagg
      541 cacatcggtt aaaaatctat tataattgtg aaacagatga tattcgtaga ttcgcattgt
      601 cacattgacg ggaaggaatt tgacgccgac cgcgaagaag tcctcaggcg cgccgaagaa
      661 gcgggcgtca gggcgatgct cgtcgtcggg acgggcgagg cgtccgattt tgaaaatttc
      721 gagcgcgccg tccgcgtcgc cgaaagccgc gaaaatattt tcgcctccat cggcgttcat
      781 ccgcacgacg cgaaaagctt tgacccggcg gcggaaaaac gtttgattga tttggcgaaa
      841 tcgagcaaaa aagtcgtcgc gtggggcgaa atcgggctcg atttttatta tgaccacagc
      901 ccgcgcccgc ttcaggcgca ggttttccgc cggcagattc atattgcgag agatttaaat
      961 ttgccgatta ttattcactc gcgcgacgcc gacgaggaaa cggtgcgcat tttaacggaa
     1021 gaatgcggca gcgaagattt tcgcggcggc attatgcatt gtttcggcgg cagcgcggcg
     1081 atggcggaag atttgatgaa aatcggtttc ctgatttcgt ttgccggcaa cgtgactttt
     1141 aaaaaagccg ggaatttgcg cgatgcggcg cgcgccgtgc cgctcgaaag gcttttaatc
     1201 gagaccgatt gcccgttttt aacgcccgtt ccgtttcgcg ggcggcgcaa cgagccgatg
     1261 tacgttgtcg aaacggcgcg atttctggcg gatttttacg ggattgaatt ggagaggctg
     1321 gcggaacgga cgacgctcaa ttttacggaa tttttcggtc tgacgctgaa aaacggcggc
     1381 tgaacaataa agctcgccgg tcgttcaacg cgcctcgtca gccgttcaac gaacatcgtc
     1441 aatcattcaa cgggctgagt aagtcgttca acgaccgatt caagtcattc aacgagccgt
     1501 tcaagtcgtt caacgagccg ttcaagtcgt tcaacgtggc gttcaagtcg ctcaatgaag
     1561 agttcgagtc gttcaacggc ggtgaaaagt tgttcaaagc gcggtaaatt tcggctaaaa
     1621 ggcgttttta ttaaggtttt cg
//
LOCUS       DASWNE010000550         1714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_615083, whole genome shotgun sequence.
ACCESSION   DASWNE010000550 DASWNE010000000
VERSION     DASWNE010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615083"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..949)
                     /locus_tag="VGB68_10940"
     CDS             complement(161..949)
                     /locus_tag="VGB68_10940"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HEY0049795.1"
                     /translation="MSEKPVEIKLPANLFQGKTAVVTGASRGVGRATVLRLAEAGANV
                     VVNYLSAEEKASSVVKLCEEKGVGAISVQGDASEWSDAQNVAQRAIEKFGQIDLLVCN
                     AGVWEGAPIEEMSEEVWNKVLNTNLKSAWAMTKACVPAIKKRESGAIVLVSSTAGQRG
                     EANYSNYAASKGGQISFTKALASELCPKIRVNAVAPGWIETAMVRPVFEDENYRQSVI
                     DSIPLKRMATTDDVALSICFLLSDWSRHITGEIVNINGGTVLCG"
     gene            complement(955..1425)
                     /locus_tag="VGB68_10945"
     CDS             complement(955..1425)
                     /locus_tag="VGB68_10945"
                     /inference="COORDINATES: protein motif:HMM:NF025447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4262 domain-containing protein"
                     /protein_id="HEY0049796.1"
                     /translation="MRTAKNEAERKTISDVKNYGLHIVYVFEDEENPGFAYSVGLYEN
                     YLHPEIIIIGLKQEISHTLLNNIAYDIKNGKNFTAGEFHEGVLDDFLCYFGELPKSKY
                     EEYVGWAMWFYKGSDFPLIQCVYPDIEGKFVWNKGFPEEAGFFCRLLIKPPAEH"
     gene            1487..>1714
                     /locus_tag="VGB68_10950"
     CDS             1487..>1714
                     /locus_tag="VGB68_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049797.1"
                     /translation="MNDVNLLENQPVAWQPSEEVIERAQLTHFMKQVGANSFDELYRF
                     SITDVEKFTTEVLHFLEIKFNPPYENLLDLSD"
BASE COUNT          429 a          446 c          370 g          469 t
ORIGIN      
        1 tgattaactt gtaaagttaa ttgaaaacgc ctgcggaagc aaaggaagcg atgaaattta
       61 cagggataac aggatggaca gggataacaa gaaattgatt ttatccctat ttatcctgtt
      121 atccctgtaa attcttttcc gtgattccct gtagcgaaaa ttagccgcat aaaaccgtgc
      181 cgccgttgat gttcacaatc tcgcccgtga tgtggcgcga ccagtccgac agcagaaaac
      241 aaatcgagag cgccacatcg tcggtcgtcg ccattcgctt tagcggaatc gagtcgatga
      301 cgctttggcg gtaattttcg tcttcgaaaa ccggacgcac catcgcggtt tcaatccagc
      361 cgggcgcgac cgcgttgacg cgaattttcg ggcatagctc gctggctaag gctttcgtaa
      421 aactgatttg cccgcctttc gacgccgcgt agtttgaata attcgcctcg ccgcgctgtc
      481 cggcagtcga agaaaccagc acaatcgcgc ccgattcgcg tttttttatc gccggaacgc
      541 aggctttcgt catcgcccag gcgcttttca gattcgtgtt cagaaccttg ttccagacct
      601 cttccgacat ttcctcaatc ggcgcgcctt cccagacgcc cgcgttacag acgagcaaat
      661 cgatctgccc gaatttttca atcgcccgct gcgccacatt ctgcgcgtcc gaccattcgg
      721 aagcgtcgcc ctgcacactg atagcgccga cgcctttttc ctcacataat ttcacgacgc
      781 tgcttgcttt ttcttccgcg ctcaaataat tgacgacgac attcgcgccc gcttccgcca
      841 atctcaaaac ggtggcgcga ccgacgccgc gcgaagcgcc cgtgacgacg gcggttttgc
      901 cttgaaaaag attggcgggc agcttgattt cgacaggttt ttcgctcata aaatttaatg
      961 ttccgccggc ggtttaatta acagacggca gaaaaatccc gcttcttcgg gaaagccttt
     1021 attccaaacg aattttcctt cgatatcggg ataaacgcat tgaatcaaag gaaaatcaga
     1081 acctttataa aaccacatcg cccaaccgac atattcttca tactttgatt tcggaagttc
     1141 gccgaaataa caaagaaaat catcgagcac gccctcgtga aactcaccgg cggtaaaatt
     1201 cttcccgttt tttatatcgt aagcgatatt gttgagcagc gtgtgcgaga tttcctgctt
     1261 cagcccgatt attattattt cgggatgcag ataattttcg taaagcccga cgctgtatgc
     1321 aaagcccgga ttttcttcat cctcaaaaac gtaaacaata tgtaagccgt aattttttac
     1381 atcggaaata gttttcctct ccgcttcgtt tttcgccgtt ctcatatttt ttcgctcgct
     1441 ttgcgtgtga tagattatgc cttattaaat tgaaaatttt aaacttatga acgacgtaaa
     1501 ccttttggaa aatcagcccg tcgcctggca gccttcggaa gaagttatcg aacgcgctca
     1561 actgacgcat tttatgaagc aggtgggcgc caattctttt gacgagctgt atcgattttc
     1621 gataaccgat gtcgaaaaat tcacgacgga agtactgcat tttctcgaaa taaaatttaa
     1681 tccgccttat gaaaatctgc tcgatttgtc ggac
//
LOCUS       DASWNE010000551         2505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_123551, whole genome shotgun sequence.
ACCESSION   DASWNE010000551 DASWNE010000000
VERSION     DASWNE010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123551"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VGB68_10955"
     CDS             complement(<1..485)
                     /locus_tag="VGB68_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY0049798.1"
                     /translation="MLKNKRKCGTRNAERGTTIRFALSCLLLTACCLLAVGCRYDMQD
                     QPRYKVYKKSDFWGERDPRASRDLPEGTVARGFLREDKALYTGKKENAGGAQQQPAQT
                     TTDASGNTVVTTFPDAIDEFPVPVTKELIDRGQDRYNIYCVVCHGPTGNGDGMIVRRG
                     FS"
     gene            complement(472..1005)
                     /locus_tag="VGB68_10960"
     CDS             complement(472..1005)
                     /locus_tag="VGB68_10960"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HEY0049799.1"
                     /translation="MDKKIYGLMAEFDTPTALVDAANKVREAGFTKTDAFSPFPLHEI
                     DAALGIRRSILPFLVFGGGILGLLSGIGLQYYTHVIDYPIIVGGRPHFSLPAFIPPAY
                     ELTILLAAFTAVFGMLFLNGLPQPYHPVFNVPRFALASRDKFFLLVEARDPKFKYEDT
                     KDFMQGLNAQEVFDVEE"
     gene            complement(1127..>2505)
                     /gene="nrfD"
                     /locus_tag="VGB68_10965"
     CDS             complement(1127..>2505)
                     /gene="nrfD"
                     /locus_tag="VGB68_10965"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEY0049800.1"
                     /translation="DFASVSDKIGDVTLKKKTPFHWFIGFGIAFVIAQLLLFTVVYLV
                     TTGIGIWGNNQPIGWAFDIINFVWWIGIGHAGTLISAILLLLNQKWRTSINRFAEAMT
                     LFAVACAGMFPLLHTGRPWLAYWLFPYPNVMNMWPQFRSPLMWDVFAVSTYATVSLLF
                     WYVGLIPDLATLRDRAKNPIFKFIYGALSMGWRGSARHWHRYEIAYLLLAGLSTPLVL
                     SVHSIVSFDFSVAQVPGWHATIFPPYFVAGAIFAGFAMVLILCIPLRNWYGMKDFITK
                     THLIYMGKVMLATGLIVVYGYAMEAFFGWYSANQYEQYMIYNRIWTGPYWWSYWLLIL
                     CNGLSIQLLWFKRFRESEFWLFLISIVVSVGMWLERFVIIVTSLHRDFLPSSWAMYTP
                     TVFDWTMFAGTIGFFFTLIYLFVRFLPIISIFEVRTLLPEANVHGHHQDFEENVNEIE
                     TFPAPKKA"
BASE COUNT          679 a          707 c          636 g          483 t
ORIGIN      
        1 gagaatccgc gacggacaat catcccgtcg ccgtttcccg tcggaccgtg acagacgacg
       61 cagtaaatgt tgtatctatc ctgcccgcgg tcgattaatt cctttgtcac cggaacggga
      121 aattcgtcaa tcgcgtcggg aaacgtcgtc acgaccgtat tgccgctcgc atcggtcgtc
      181 gtttgcgccg gttgctgctg agcgccgccc gcattttctt ttttgcccgt gtagagcgcc
      241 ttgtcttcgc gcaaaaatcc gcgcgccacc gttccttccg gtaaatcgcg cgaggcgcgc
      301 gggtcgcgct cgccccagaa atcgctcttt ttataaacct tataacgcgg ctggtcctgc
      361 atatcgtagc gacagccgac agccagcagg cagcaggcgg taagcaggag acaggaaagc
      421 gcaaagcgga ttgtcgttcc gcgctccgcg ttccgcgttc cgcattttcg cttattcttc
      481 aacatcgaaa acctcctgcg cattcagacc ttgcataaaa tctttcgtat cctcgtattt
      541 aaatttcggg tcacgcgcct cgaccagcag aaaaaactta tcgcgcgaag ccagagcaaa
      601 acgcggcacg ttgaaaaccg gatgatacgg ctgcggcaaa ccgttcagaa acaacattcc
      661 gaagacggcg gtaaacgccg ccagcagaat cgtcaattca taagcgggcg gaataaacgc
      721 cggcaaactg aaatgcgggc gaccgccgac gataatcggg tagtcgataa cgtgcgtgta
      781 atactgcaat ccgattcccg acaacaagcc cagaataccg ccgccgaaaa ccagaaaagg
      841 taaaatcgaa cgcctgatgc cgagcgcggc gtcgatttcg tgcagcggaa acggcgagaa
      901 agcgtcggtt ttcgtaaagc cggcttcgcg cactttattt gccgcatcga ccagcgcggt
      961 cggcgtatca aattctgcca tcaaaccgta aatcttctta tccatatctc tgttcgcttt
     1021 cgcgtcaccc gcagccgccg aatgttggca gtcgaagcga cctgggtgag tggtcttaaa
     1081 atcgactgcg gacagtgggc ggctgccggc aaccttttac aaaaatctac gcctttttcg
     1141 gcgcgggaaa cgtttcaatc tcgttgacgt tctcttcaaa atcctgatga tgcccgtgca
     1201 cgttggcttc gggcaaaagc gttctgacct cgaaaatcga gataatcggc aaaaaccgga
     1261 caaacaggta aatcaaggta aagaaaaacc cgatagtgcc ggcaaacatc gtccagtcaa
     1321 aaaccgtcgg cgtatacatc gcccacgaag acggcagaaa atcgcggtgc aggctcgtca
     1381 cgataatcac gaagcgttcg agccacatac cgacgctgac gacgatcgaa atcaggaaca
     1441 gccagaattc gctttcgcga aaacgcttaa accagagcag ctgaatcgac aacccgttgc
     1501 acagaatcag aagccagtac gaccaccagt acggacccgt ccagatgcgg ttgtaaatca
     1561 tatactgctc atactggttg gcgctgtacc agccgaaaaa cgcttccatc gcgtaaccgt
     1621 aaacgacgat caagccggtc gccagcatga ctttgcccat gtaaatcaag tgcgtcttgg
     1681 tgatgaaatc cttcattcca taccagttgc gaagcggaat acacagaatc aaaaccattg
     1741 cgaagcccgc gaaaatcgcg cccgccacga aatacggcgg gaaaatcgtc gcgtgccagc
     1801 ccggaacctg cgccaccgag aagtcgaagc tgacgatgga gtgaaccgaa agcacgagcg
     1861 gcgtcgaaag tccggctaaa agcaagtaag cgatttcata tcgatgccag tgccgcgccg
     1921 aaccgcgcca gcccatcgaa agagcgccgt aaataaattt gaaaatcgga tttttcgcgc
     1981 ggtcgcgcaa ggtcgccaaa tcgggaatca agccgacgta ccagaacaaa agcgaaacgg
     2041 tcgcataggt cgaaaccgca aaaacgtccc acatcaaagg cgagcgaaat tgcggccaca
     2101 tattcatcac gttcgggtac gggaaaagcc agtaggcgag ccacgggcgt cccgtatgca
     2161 agagcggaaa catgcccgcg caggcgaccg caaaaagcgt catcgcttcg gcaaaacggt
     2221 taatcgacgt gcgccatttc tggttgagaa gaagcaaaat ggcggaaatc agcgttcccg
     2281 cgtgaccgat gccgatccac cagacgaagt tgataatgtc aaacgcccag ccgatcggct
     2341 gattgtttcc ccagattccg atacccgtcg tgaccagata caccaccgtg aaaagaagca
     2401 gttgagcaat cacaaacgca attccgaaac cgataaacca gtgaaacggc gttttctttt
     2461 ttaaggtgac gtcgccgatt ttgtctgaaa cgctggcaaa atcgt
//
LOCUS       DASWNE010000552         1960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_195830, whole genome shotgun sequence.
ACCESSION   DASWNE010000552 DASWNE010000000
VERSION     DASWNE010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195830"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VGB68_10970"
     CDS             <1..344
                     /locus_tag="VGB68_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEY0049801.1"
                     /translation="DEHYRTAQDVKRILQRYKDLQDIIAILGLDELSEDDKLIVARAR
                     KIQRFFSQPFTVGEQFTGLKGKYVEVADTIKGFREILDGKCDDMPEQSFYMVGTIEEA
                     REKAKSMAAAA"
     gene            437..832
                     /locus_tag="VGB68_10975"
     CDS             437..832
                     /locus_tag="VGB68_10975"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEY0049802.1"
                     /translation="MLRLEIVTPEKKVVDETVDAVTVPGANGEIGILPNHAPLISTLK
                     PGILAYTRGGATERLVISGGFIEVSADKVSVLADVAETADEIDVEAARSEREATEKAL
                     GAWSGSEADFEIAKERLERAQARLQLAGR"
     gene            1041..1940
                     /locus_tag="VGB68_10980"
     CDS             1041..1940
                     /locus_tag="VGB68_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY0049803.1"
                     /translation="MKTINQFVKTARQSMILFLFVLAVFLLNAQAQTVLVNYDFASAV
                     AGTPCTASPLTTAPGVTSTFTTGGTGGGTCTTPAGTAATAPPAFVANAANQSVSLTSF
                     AAGSTNFFQFQLSGVGSYTDYMLFFQSQRSGTGPVNLDVQYSTNGTTFTTFQTVNPGN
                     GVFAAFNIDLSAVPAIENQPTVYFRLVGSGGTGAAGTLRIDNFQVQAMSVSAATVTVG
                     GRVTNAGGYGIGNVVVSINGGSLSEPIYARTSPFGYYSFEGIAAGQTYIVTVSSKTHT
                     FNPSSRIIDVQDSVGNADFVSQE"
BASE COUNT          503 a          470 c          506 g          481 t
ORIGIN      
        1 gcgacgagca ttatcgaacg gcgcaggacg tgaaacgcat cttgcagcgt tacaaggatt
       61 tgcaggacat catcgccatc ttaggtctcg acgaattgag cgaagacgac aagctcatcg
      121 tggcgcgcgc gcgtaagatt cagcgctttt tctcgcagcc gttcacggtc ggcgagcagt
      181 ttacgggctt aaaaggcaaa tacgtcgaag ttgcggacac catcaaaggc ttccgcgaaa
      241 ttctcgacgg caaatgcgac gatatgccgg aacaatcctt ctatatggtc ggcactatcg
      301 aagaagcccg cgaaaaagcc aaatcgatgg cggcagccgc ttaagaaatt acgaattatc
      361 aattacgaat tacgaatcgg aagaatcttc aacgtcgtaa ttcgtaattt gaaattcgta
      421 attcgtaatt gaatttatgt tgagattaga aattgttact cctgaaaaaa aagtcgtcga
      481 cgaaacggtc gatgcggtga cagtgccggg cgcgaatggc gaaatcggta ttttgccgaa
      541 tcacgcgccg cttatttcca cgcttaagcc gggcattttg gcttacacgc gcggcggcgc
      601 gaccgaaaga ttagtcattt ccggcgggtt tatcgaagtc agcgccgata aagtttcggt
      661 tttagccgac gtggcggaaa ctgccgacga aatcgacgtc gaagcggcgc gttccgaacg
      721 cgaagcaacc gaaaaagctc tcggcgcctg gtctggctcg gaagcggatt ttgagattgc
      781 aaaagaaaga ctggaacggg cgcaggcgcg tctacagtta gccggaagat aaagctttgt
      841 ttaattaaat ttaataaaaa cgaatcgagc gtgaaaatgt ttcggttctt ttttttaatg
      901 tatttgtaac ttgtctattt tagaaaaata gttgttatta aacagattgt ttactataat
      961 ctccctatac tttaaaaccg ttaccgcaat tttttctaaa gcttggatta gaacaattga
     1021 gtttcgaaag aggggaatat atgaaaacta tcaatcaatt tgtcaaaacc gcccgccaat
     1081 caatgatttt atttttattt gtattggcgg tttttttatt aaacgcgcag gcgcagaccg
     1141 tgctcgtaaa ttatgatttt gcttctgccg ttgccggaac gccgtgcacc gcgtcgccgc
     1201 tcacgaccgc tccgggcgta acgtccacat tcacgacggg cggaaccggc ggcggaacct
     1261 gcacgactcc ggcgggaacg gcggcgactg ctccgccggc atttgtggct aacgcggcta
     1321 atcaatcggt cagtctcaca agctttgcgg caggctcgac gaatttcttc cagtttcaat
     1381 tgagcggcgt cggcagctat acggattata tgctgttctt ccagtcgcag agaagcggaa
     1441 ccggtccggt caatcttgac gtccaataca gcacgaacgg aacgactttt accacttttc
     1501 aaacggttaa tccgggcaac ggcgtgtttg ccgcatttaa tattgatttg agcgccgttc
     1561 cggcaatcga aaatcaaccg accgtctatt tcagactagt cggctcaggt ggcacgggcg
     1621 ctgccggcac gctgagaatc gacaattttc aggttcaggc aatgagcgtc tcagccgcga
     1681 ccgttacggt cggcgggcgc gtgacgaacg cgggcggcta cggaatcggc aacgtggtcg
     1741 tttccattaa cggcggcagt ttgtccgaac cgatttacgc gcgaaccagc ccgttcggtt
     1801 attacagttt cgagggcatc gccgccggtc agacgtatat cgtcacggtt tcttccaaga
     1861 cacacacctt taatccttcg agccggatta tcgacgtgca ggacagcgtc ggcaatgcgg
     1921 attttgtcag tcaggaataa cgacgaaata cgaacttaat
//
LOCUS       DASWNE010000553         1912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_370451, whole genome shotgun sequence.
ACCESSION   DASWNE010000553 DASWNE010000000
VERSION     DASWNE010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370451"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="VGB68_10985"
     CDS             complement(<1..974)
                     /locus_tag="VGB68_10985"
                     /inference="COORDINATES: protein motif:HMM:NF015912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
                     /protein_id="HEY0049804.1"
                     /translation="MNEYDEKDDLMDRLLELGRDKKNFLSYDDLNREMPDSMVSPEDI
                     EDVLQKLEGTGISISDSDSALLEQAASLAIDDVEGLDEEDLDLDLSAGTLDKTNDPVR
                     LYLREMGVVPLLTREGEVTIAKRIERGQIKTQKAISRSPIAVEQLVFLGAELGRGRLS
                     IREIVTFSEQAELTGEEDKADEYLRWTIEGIENVRINYEEALGVLGQFREEQKKSKGK
                     QTKKLIHLRRQVARLRLEIAQEVKNLNLTERAKQRLIKAIQKVADEIRKAEREIAKSE
                     DKLEGKVSPADKKQLTQKITDARAKLIELESKYHISPVEVKRSLQAIL"
     gene            complement(1127..>1912)
                     /locus_tag="VGB68_10990"
     CDS             complement(1127..>1912)
                     /locus_tag="VGB68_10990"
                     /inference="COORDINATES: protein motif:HMM:NF021895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049805.1"
                     /translation="NGVDSYNIQRGNAFPYLQFVLENATRERNLALPKQKADAIEDVL
                     PVISAVRNSIQKRESFDQAMNYLRVEDAVLKRDLWKTVKLGAHVDAGTIRKTVSRTMQ
                     ARLTVAEQRLLEFIIHDRELREVILPQLEETDYEALATASVFRALLTIQQDGKQFTAE
                     NLENLVSDDLQVEEVLPLLLMAAPPREPDEAIDEILAEAEKCVFTLRGMAISNRILEI
                     SQGLVNAEQTGDFELRDRLVREQIDLARLKYELEKANLLSAEN"
BASE COUNT          402 a          521 c          458 g          531 t
ORIGIN      
        1 attaaaatcg cctgcaaaga gcgtttcacc tctaccggcg aaatgtgata tttgctttca
       61 agttcaatca gcttggcgcg cgcgtccgtt atcttctgcg tcaactgttt tttatcggcg
      121 ggcgaaacct ttccttccag cttgtcctcg gatttggcga tttcgcgctc ggctttcctg
      181 atttcgtcgg cgaccttctg aatcgcttta atcaggcgtt gtttggcgcg ctcggtcaga
      241 ttcaggtttt taacttcctg cgcgatttcc agacgcaggc gcgccacctg ccggcgcagg
      301 tgaatcaatt ttttggtctg cttgcctttc gattttttct gctcttcgcg aaattgaccg
      361 agaacgccca aagcttcctc gtaattgatg cggacgtttt cgatgccctc aatcgtccag
      421 cgcagatatt cgtccgcctt gtcttcctcg cccgtcaatt cggcttgctc ggaaaacgtg
      481 actatttcac gaatgctgag tctgccgcgc ccgagctccg cgccgagaaa cactaattgc
      541 tcgacggcaa tcggcgaacg cgaaatcgct ttctgcgttt tgatttgacc gcgctcgatg
      601 cgcttggcga tagtgacttc gccctcgcgc gtcaaaagcg gcacgacgcc catttcgcgc
      661 aggtaaaggc gcaccgggtc gttggttttg tccagcgttc ccgccgaaag gtctaaatcg
      721 aggtcttcct cgtccagacc ctcgacgtcg tcgatggcga gactggcggc ttgttcgagc
      781 aaagccgaat cggaatcgga aatcgagatt cccgtccctt ccagcttttg caaaacgtcc
      841 tcgatgtctt cgggcgagac catcgaatcc ggcatttcac gattcaaatc atcgtagctt
      901 aaaaagtttt tcttatctct gccgagttcc agcagtctgt ccattaaatc gtctttttcg
      961 tcgtattcgt tcattttgtt taataaaaag ttgtaataag agcttggatt aacaatcaac
     1021 ggaattcgtt gtttgctaat tcaaggttcg ccggagagga ttcgggattc agaattcagg
     1081 atttaaaatt cggaatcttg aatcctgaat tctgaatcct ctccggctaa ttttcggcgc
     1141 tgagcaagtt tgctttctca agctcgtatt tcagccgcgc caaatcgatt tgctctctga
     1201 ccagccggtc gcgcaattca aaatcacccg tttgttcggc attgacgagt ccctggctta
     1261 tctctaaaat ccgattcgaa attgccattc cgcgtaaagt gaacacgcat ttttcggctt
     1321 cggctaaaat ctcgtcaatc gcctcgtcag gctcgcgcgg cggcgctgcc attaaaagaa
     1381 gcggcaaaac ttcttcgacc tgtaaatcgt cggaaaccag attttccaga ttttccgccg
     1441 taaactgttt gccgtcctgc tgtattgtca aaagagcgcg aaacacggaa gcggtcgcca
     1501 gcgcttcata atcggtctct tcgagctgcg gcaaaatcac ttcgcgcagc tctcggtcgt
     1561 gaataataaa ttccagaagc cgctgctcgg caaccgtcag gcgagcctgc atcgtgcgcg
     1621 agacggtttt gcgaatcgtt cccgcatcga cgtgcgcgcc gagcttgacc gttttccaca
     1681 aatcgcgttt caagaccgcg tcctcgaccc gcagataatt cattgcctgg tcgaaagatt
     1741 cgcgtttctg aatcgaattg cgtacagccg aaataacggg cagcacatcc tcgattgcgt
     1801 cggctttttg cttcggcaat gcgagattgc gctcccgcgt cgcattttcc agaacgaatt
     1861 ggagataggg gaaagcgttg cctctttgaa tgttatagct gtcaacgccg tt
//
LOCUS       DASWNE010000554         4866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_477198, whole genome shotgun sequence.
ACCESSION   DASWNE010000554 DASWNE010000000
VERSION     DASWNE010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477198"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1704
                     /locus_tag="VGB68_10995"
     CDS             <1..1704
                     /locus_tag="VGB68_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049806.1"
                     /translation="YALDKINSGPAILTIDEENGVWTALAKTGKLAHLSNEAVTLYDI
                     GIESRPVGLAIGTRANKHQGAIWIAASYDNKLIRFDTVTKQTREYKIQGENSWPFNVA
                     IGPNGEIWFTQRASGRIGLLDPNTGNTTHYDIQTPNCGPAGLAVDQKTGDVWFTESYA
                     DRIGRLNPKTGEIKEYSMGEKSTGLVSGPAGLAVDREGNVWFAKLEGKIGYLARGAET
                     IELIDVPTESRRPAGITVSPNGDVWAVALDGNLLLRYQPPTREFIQYPLPTGEPDNQP
                     GVPPFAKTSRPFGIGVDSQGNVWFSEQYTGQLGVLDMASPSVELFSPMKSVRVADPLL
                     TLRISERVSGVAEVAVLLDGKPASVIRGHLELYKILPGKHTLKIAVTDGAGYRTQITK
                     EFEYAPGQVAMQGWMNRLIPKNKKGEALKNELQIAAKDAAKGNYRENIAKISQKLEQN
                     ANLFETYNKRSLTALMDFQNLTAGKTVEVRILDDAPFYSQANVVLYSGDSVVWKYDPP
                     SNGHSISHKLQRIEIQGLGIRSNLIRAGESFSHRFDKPGEYQIKNTENQSVAIVKVVS
                     R"
     gene            1701..2471
                     /locus_tag="VGB68_11000"
     CDS             1701..2471
                     /locus_tag="VGB68_11000"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEY0049807.1"
                     /translation="MNFDNFRIIDLSVPLAPCPSENVPVEIQYMDHRFGGAHLAQLVN
                     IEQESLCDGLGWASERVTAITHSGTHVDSPFHYSPVCAGEPSRTIDEMPLDWFWGAGV
                     CITVDDKPQSRSIGVDEVEAFERQHDYRIKPGDIVLFRTGAGSRYGSPDYNRHGRGIS
                     PELIEMLCLKGVRVFGTDAWSIDPPYWLMQENISKHGTGAVWEAHFAGRQREFCVIEK
                     LSNLDKLPPAGFWVSCFPIKVKRGSGGWTRAVAFLNRQ"
     gene            2500..3609
                     /locus_tag="VGB68_11005"
     CDS             2500..3609
                     /locus_tag="VGB68_11005"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY0049808.1"
                     /translation="MIYKCKLLVTLVFAAIAALYLTFEVSRAQQITKSAQDEAATAGQ
                     KLVQEKCSSCHTLSGSSNRKDALDVARKEAPSLAFAGSKFRQEWLEKWLVAPVAVRPA
                     GFLSFRFVDASLTGDRVNDSRIPAHPALSGEEAKIAAGYLMSLKQAVSAVTPGEVNPN
                     SRPHILFEKVLGCGACHQVEPGKGGLSAPELFTAGRRLNREWANAFMADPPAWAGATM
                     PKAEMRPEQLALISNYVFSANQSAPEISSVKTAKPVKAPVQLPAERAEGLYKVYCSQC
                     HGVSGNGKGINAPFLFVAPRNHTSFDEMSILNDDRISAAVKFGGAAVGKSSLMPAWNG
                     ILSDADIQLLVVYLRRLSGTQAALDYQNNSLRATE"
     gene            3613..4854
                     /locus_tag="VGB68_11010"
     CDS             3613..4854
                     /locus_tag="VGB68_11010"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEY0049809.1"
                     /translation="MKKAVITGIGPVTPIGVGINSFWKSAIEGASGTSALTSLPLNFP
                     VETFSSRIVAAVKDEWLPAADESEDDARFFRFGSLACELALKDAGLEDIRGDKTAVVL
                     GNAVGGTTPMEASYLSMKDGDTLDRRKTPYKLLKQMSFHYMAHEIAEKYGCEGTTLTV
                     STGCTAGVDAVAAGFELIKYGKAQTVIVGAAEAPITPVVYAAFDVIGALSKNNANPLE
                     ASRPFDKDRDGFVLGEGAGFLILEEKNQAIRRNARIYAEITGYASVSNSYHMTDLPAE
                     GTALADCMKLALEDAGLAAEDIDHINAHGSSTPQNDICETNAIKNVFGRHAQNLTVNS
                     LKAIIGHALGASNAIEIAACAAAIERQHMFPTAHLDAPGDGCDLDYIPKQSRRMNVRH
                     LLKLSSGFSGIHSALVMSAPF"
BASE COUNT         1220 a         1242 c         1344 g         1060 t
ORIGIN      
        1 tacgcattag acaaaataaa cagcggaccg gcaattctga ctattgacga agaaaacggc
       61 gtctggacgg cgctggctaa aaccggaaag ctggcacatt tatcaaatga agccgttact
      121 ttatacgata tcggaataga aagccgcccg gtcgggcttg caatcggaac gcgcgcgaat
      181 aaacatcagg gcgcaatctg gatagcggcg agctatgaca ataaactcat ccggttcgat
      241 acggtcacaa aacagacgcg ggaatataaa atacaaggcg aaaatagctg gccttttaac
      301 gtcgcaatcg gaccgaacgg ggaaatctgg tttacgcagc gcgcttccgg cagaatcggc
      361 ttgcttgacc cgaatacggg caataccact cattatgata ttcaaactcc gaattgcggt
      421 cctgccggat tagccgtcga ccaaaaaacg ggcgacgttt ggtttacgga aagctatgcg
      481 gatcgcatcg gcaggcttaa cccgaagacg ggtgagatta aagaatattc gatgggagaa
      541 aaaagcaccg ggctggtcag cggacccgcc gggctggcag ttgatagaga gggcaatgtc
      601 tggtttgcga aattagaagg aaaaatcggg tatctcgcgc gcggcgcgga gacaatcgaa
      661 ctgatagatg ttcctacgga gtccaggcgc ccggcgggaa ttacggtttc gccgaatggc
      721 gatgtctggg cggtggcgct ggacggaaat cttctgctca ggtatcaacc tccgacgcgc
      781 gaatttattc aatacccgct gccgaccggc gagccggaca atcaaccggg cgttccgccg
      841 tttgccaaaa cctccagacc tttcggaatc ggcgtcgata gtcagggaaa cgtctggttt
      901 tccgagcaat atacgggtca gctcggcgtg ctcgatatgg cttcgccttc cgtcgagttg
      961 ttttctccga tgaaaagcgt tcgcgtcgcc gatccgcttc ttaccctgcg gatttcagaa
     1021 agagtttcgg gcgtcgccga ggttgccgtt cttctggacg gtaaacccgc gagcgtcata
     1081 agaggtcatc tggaacttta taaaattctt cccggaaaac atactctgaa aatagcggtt
     1141 acggacggag cgggctatcg cacgcaaata accaaagaat ttgaatatgc gcccgggcaa
     1201 gtcgccatgc agggctggat gaaccgcctg atacctaaaa ataaaaaggg agaagcgctt
     1261 aaaaatgaac tgcaaatcgc ggctaaagat gcagccaaag gtaattaccg cgaaaatatc
     1321 gcaaaaattt ctcagaagct cgaacaaaac gcgaatctct ttgaaacata taacaagcgt
     1381 tctttgactg ccctgatgga tttccagaat ctgaccgcgg gtaaaacggt cgaggttcgc
     1441 attctggacg acgcgccctt ttattctcag gcaaacgtcg tgttgtattc gggcgattcg
     1501 gtcgtctgga aatacgaccc tccaagcaac ggacattcga tttcgcacaa attacagcgc
     1561 atagaaattc aagggcttgg aatacgctcg aatcttatcc gggcgggaga aagtttttct
     1621 caccgcttcg ataaaccggg cgaatatcaa atcaaaaata ccgaaaatca atccgtcgcg
     1681 attgtaaagg tggtctcgcg atgaacttcg ataacttcag gataatcgat ttgagcgttc
     1741 ctttagcgcc gtgtccttcg gaaaacgtcc cggtcgaaat tcaatatatg gatcaccggt
     1801 tcggcggcgc tcatctggcg cagctggtca acatcgaaca ggaaagtctg tgcgacgggc
     1861 tcggctgggc gagcgagcgc gtaaccgcaa ttacgcactc cggaacgcat gtcgattcgc
     1921 cgtttcacta ttcgccggtt tgtgcgggag agccgagccg caccatcgac gagatgccgc
     1981 tggattggtt ctggggcgcg ggcgtttgca tcacggtcga cgacaagcct cagagccgat
     2041 ccatcggcgt cgatgaagtc gaagcttttg aaagacaaca cgattaccgg ataaagcccg
     2101 gcgatatcgt cctgtttcgg acgggcgcgg gcagtcgata cggctcgccc gactacaaca
     2161 ggcacgggcg cggaatatcg ccggaattga tagaaatgtt gtgtctaaaa ggcgtcagag
     2221 ttttcgggac ggacgcctgg agcatcgacc cgccttattg gctgatgcag gagaatattt
     2281 cgaaacacgg aaccggcgca gtctgggaag cgcattttgc cggtcggcag cgtgaatttt
     2341 gcgttatcga aaagctttcc aatttagata agctgccgcc cgccggtttt tgggtttcgt
     2401 gttttcccat caaggtcaaa cgcggaagcg gaggctggac gcgagccgtc gcctttttga
     2461 atcgtcaatg attttgcgaa atgaggtgga aagccgtcta tgatatacaa atgcaaactg
     2521 ctggtgactc ttgtttttgc tgcaatcgcc gcgctctatc tgacattcga agtttcgagg
     2581 gcgcagcaga taaccaaatc cgcacaggat gaagccgcaa ccgccgggca gaaactggtg
     2641 caggaaaaat gcagttcctg tcacacgctt tccggctcgt caaatcgaaa agacgcgttg
     2701 gacgtggctc gaaaagaagc cccttctctg gcttttgcgg gcagcaaatt tcgtcaggaa
     2761 tggttggaaa aatggctggt cgcacctgtt gccgtcagac ctgccggttt tttatccttt
     2821 cgctttgtag atgcctcgct gaccggagac cgcgtaaatg attcgcgcat tcccgctcac
     2881 ccggcgctga gcggcgagga ggcaaaaata gcagccggtt atttaatgag cctgaaacaa
     2941 gcggtttcgg cggtgacgcc gggcgaggtc aatcctaact cgcgcccgca tattttattt
     3001 gaaaaagttc tcggctgcgg cgcttgtcac caggtcgaac ccggcaaagg cggcttgtcc
     3061 gcgccggagc ttttcaccgc cggtcgccgg ctcaatcgcg aatgggcgaa tgctttcatg
     3121 gcagacccgc cggcgtgggc gggcgcgacg atgcccaaag ccgaaatgcg acccgagcag
     3181 ttggctttaa taagcaatta tgtattttcg gctaatcagt ccgctccgga aatcagttcc
     3241 gttaaaacgg caaaacccgt aaaagcgccc gttcaattgc cggcggagcg ggctgaaggc
     3301 ttatataaag tttattgctc gcagtgtcac ggcgtcagcg ggaacggtaa aggcataaat
     3361 gcgccgtttc tttttgtcgc gccgcgcaat cacacctcgt ttgacgaaat gagtatttta
     3421 aacgacgacc gcatttcggc agccgtcaaa ttcggcgggg cggcggtcgg gaaatcatct
     3481 ttaatgccgg cgtggaatgg aattctttcc gacgcggata ttcagctttt agtcgtttat
     3541 ctgcgccggc tcagcggaac gcaagccgct cttgattatc agaacaacag cctgagggcg
     3601 accgaataaa ttatgaaaaa agcagtaatt acaggtatcg gacccgtgac gccgatcggc
     3661 gtcggaataa attctttctg gaaaagcgcg attgaaggcg caagcggaac gtccgcgctg
     3721 accagccttc ctttaaactt tccggtcgag acgttcagtt cgcgaatcgt ggcagccgtc
     3781 aaggacgaat ggctgcccgc tgccgacgag tcggaagacg acgcgagatt ttttcgattc
     3841 ggaagcctgg cgtgcgagct ggcgctgaaa gatgccggat tggaagacat tcgcggagat
     3901 aaaacggcgg ttgttttggg aaacgccgtc ggcggaacga cgccgatgga agccagctat
     3961 ttgtcgatga aagacggcga tacgctcgac cggcgaaaaa ctccttacaa attgctgaaa
     4021 cagatgtctt ttcattatat ggctcacgaa atagccgaaa aatacggctg cgaaggcacg
     4081 accctgaccg tctcgacggg ctgcaccgca ggcgtggacg cggtggcggc gggctttgaa
     4141 ttgattaaat acggcaaagc gcagacggtc atcgtcggcg ccgccgaagc tccgataact
     4201 cccgtggttt atgcggcgtt tgacgtaatc ggcgcgcttt ctaaaaataa cgccaatccg
     4261 ctcgaagcct cacgtccttt tgataaagac cgcgacggat ttgttttggg cgagggagca
     4321 ggctttttga ttttagagga aaaaaatcag gcgattcggc gaaacgcccg catttacgcc
     4381 gaaataaccg gctatgcttc cgtcagcaat tcctatcata tgaccgacct tcccgccgaa
     4441 ggaaccgctc tagccgattg catgaagctg gcgctcgaag atgccgggct tgccgccgaa
     4501 gacatcgacc atataaacgc tcacggcagt tcgacgccgc aaaacgatat ttgcgagacc
     4561 aacgccataa aaaatgtttt cggaagacac gctcaaaatc taaccgtaaa ctccttgaaa
     4621 gccatcatcg ggcatgctct gggagcttca aacgccatcg agattgccgc ctgcgctgcg
     4681 gctatcgaac gacagcatat gtttccgacc gctcatctgg acgcgcccgg cgacggttgt
     4741 gatttggatt atatacccaa gcaaagtcgc cggatgaacg tgcggcatct gctcaagctg
     4801 agcagcggtt tttccggcat tcactccgcc ctggtaatgt ccgcgccatt ttaaaaacag
     4861 tccgac
//
LOCUS       DASWNE010000555         1763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_477219, whole genome shotgun sequence.
ACCESSION   DASWNE010000555 DASWNE010000000
VERSION     DASWNE010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477219"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="VGB68_11015"
     CDS             complement(<1..995)
                     /locus_tag="VGB68_11015"
                     /inference="COORDINATES: protein motif:HMM:NF013479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HEY0049810.1"
                     /translation="MSSSSGEKTEKPTDKRLRDARKKGQVAKSQDLSSATLMLAAVAV
                     VWLLGGFIGGILQASVKQQIEFAAGFKGDLTNQVLYDFLWQGMTVFFWVLMPLFLVVV
                     VFAILSNYLQVGSIFSFESISPKFEKLNPAENFKQKFFKARPYIELGKTILKMTITAF
                     VAGYVLWGAREDVVRLIDKPADVAAYYTFSLVLEIGLKIGLAFLILGGADFFLQRLLH
                     SRELMMTKHEVKEEYKETEGNPQIKAQRRYLHREILSQSMAVAVREADVVVTNPTHLA
                     VALKYDRGNSEAPVITAKGADLMAAQIRQLASEAGVPMKRDVPLARALYQFDVEQ"
     gene            1249..1734
                     /locus_tag="VGB68_11020"
     CDS             1249..1734
                     /locus_tag="VGB68_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049811.1"
                     /translation="MFNKNKFLVAVLSIFFAACLNASAQQTVFDLEGNVRREVAVPAD
                     VIAVLKSDERVDACFREKGEGANEAAWFAASEIDLNGDRKMDLIIKAKDGCLFGANQG
                     PFWIFQKMPDGYQQVFSASGLQLAVLPKKVNSFSQIKISKVVSQKPTSKIYSFKSGKY
                     Q"
BASE COUNT          448 a          454 c          417 g          444 t
ORIGIN      
        1 tcctgttcga cgtcgaattg gtaaagggcg cgcgccagcg gcacgtcgcg tttcatcggc
       61 acgcccgctt cgctcgccag ttggcggatt tgcgccgcca tcaaatccgc gcctttcgcc
      121 gtaatgacgg gcgcttccga gtttccgcgg tcgtatttca gggcgacggc aagatgcgtc
      181 gggttcgtca ccacgacgtc ggcttcgcgg acagccaccg ccatgctctg cgacagaatc
      241 tcgcgatgca gataacgtct ctgggctttt atctgagggt ttccttcggt ttccttgtat
      301 tcttccttga cctcgtgctt ggtcatcatc agctcgcgac tatgcagcag tctttgcaga
      361 aaaaaatccg cgccgcccag gattagaaac gccaagccga tttttaagcc gatttccaga
      421 accagactga aagtgtaata agccgccacg tcagccggtt tatcaatcag ccgcacgacg
      481 tcttcgcgcg cgccccacaa aacgtatccg gcgacgaacg ccgtaatcgt cattttcaaa
      541 atcgttttgc cgagctcgat atacggtcgc gccttaaaga atttctgttt gaaattttcc
      601 gccggattga gcttttcaaa tttcggcgag attgattcaa acgagaaaat cgagccgacc
      661 tgcaaataat tgctcaaaat cgcaaaaacg acgacgacga gaaaaagcgg cattaaaacc
      721 cagaaaaaaa cggtcattcc ctgccagaga aaatcgtaga gcacctggtt cgtcaaatcg
      781 cctttaaaac cggcggcaaa ttcgatttgc tgtttgaccg aggcttgcag aatgccgccg
      841 ataaaaccgc cgagcagcca cacgacggcg accgccgcca gcatcagcgt cgccgacgac
      901 aaatcctggc ttttcgccac ctgacctttt tttcgggcgt cccgcagccg cttatcagtt
      961 ggtttttcgg ttttttcccc tgaactgctc gacataaatt aaaacaaatt ttaagctcgg
     1021 ctcgcttttc tatgttttcg gtcgaaatcg cgctttgttg caagtttatt taattgcctg
     1081 ccgcgccgaa aacgaaattt ttttcaactc tttaatgtta acaagtataa tacattctgg
     1141 atttcttttg acaatttatt tcgatttaat aaaagtttaa gacgctttga aaagcccgta
     1201 caaccgcgat ttttttcgcg taatctaaat cgccggagga aaaagcccgt gtttaataaa
     1261 aataaatttc tggtcgccgt tttgagtatt ttttttgccg cttgtttaaa cgccagcgcg
     1321 cagcagaccg ttttcgacct tgaaggaaac gtgcgcagag aagttgccgt gcctgccgac
     1381 gtgattgccg ttttgaaatc cgacgaaaga gtcgacgcct gttttcgcga aaaaggcgag
     1441 ggcgcgaatg aagccgcgtg gtttgccgct tccgagattg atttgaacgg cgaccgcaaa
     1501 atggatttga ttatcaaagc caaagacggc tgtctgttcg gcgcgaatca gggaccgttt
     1561 tggatttttc agaaaatgcc ggacggctac cagcaggttt tcagcgccag cggcttacag
     1621 ctcgccgtgc tgccgaaaaa agtcaattcc ttcagccaaa tcaaaatcag caaagtcgtc
     1681 agccagaaac cgacaagcaa aatttacagc ttcaaaagcg gaaaatatca ataagcggta
     1741 agcggtaaac ggtaagtggt aag
//
LOCUS       DASWNE010000556         4879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_654739, whole genome shotgun sequence.
ACCESSION   DASWNE010000556 DASWNE010000000
VERSION     DASWNE010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654739"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="VGB68_11025"
     CDS             complement(<1..867)
                     /locus_tag="VGB68_11025"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY0049812.1"
                     /translation="MEKVATVEQRKSGKTNWVTIVPIAIFHVMAVWALFTFSWTNLGV
                     GLVAWWLAGSLGIGVGFHRQLTHRGFETPDWLKHILAVFGSMALQGSPNDWVTTHRVH
                     HAFCDTDKDPHSPRHGTYWAHMGWVTVGTSQGNPVEVERRFIPDLLKDKFLVTLSNYW
                     YVPTITLGVILGFVGGWSMVLWGVFLPVTLGWHFTWFVNSVTHVWGSRRFETKDDSTN
                     NALVAAVSFGEGWHNNHHANPTSVRHGLTWYEFDVNWLQIKALEKLGLAWNLKEFNLK
                     EHEAKKASKIEDF"
     gene            1070..1426
                     /locus_tag="VGB68_11030"
     CDS             1070..1426
                     /locus_tag="VGB68_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049813.1"
                     /translation="MQYSPLAIKRRYILGNLADKCDVINKIPVYLNDKSGEPIGFADE
                     SMGRYADAFLFHLPEDVCKKLSTNGYELEIDYDVSKEDGNAGKSERLTLNYIILTSKA
                     INSAIPRRHPFTKAAE"
     gene            complement(1464..2321)
                     /locus_tag="VGB68_11035"
     CDS             complement(1464..2321)
                     /locus_tag="VGB68_11035"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY0049814.1"
                     /translation="MQNIIESESLSKNINWKNLSIVITFHVLAIAALFTFSWENLAVM
                     LVGNWIVGSLGVGLGYHRLLTHRSFKAPGWLEYTLTIFGALAIQDDAPKWVATHRIHH
                     QFTETDKDPHSTRPGFWWAHMEWILKGTAQDHDRETLKRYVPDLLKDKFHVLLARFYY
                     VPLLASIVILFAAGGWSMVLWGVFARVVFGWHSTWLVNSATHFWGERRFETKDDSTNN
                     WIIALLTFGEGWHNNHHAQPRSARHGLKWYEFDMNWLTISLFEKLGWAKEIRRFETKK
                     APAELKRAS"
     gene            complement(2492..3637)
                     /gene="mqnC"
                     /locus_tag="VGB68_11040"
     CDS             complement(2492..3637)
                     /gene="mqnC"
                     /locus_tag="VGB68_11040"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HEY0049815.1"
                     /translation="MNSNIQPILDKALAGERLTAEDAAALLESNDFVRIGLAAHEIRM
                     RKNPTDVVTYIIDRNINYTNVCNVVCTFCAFYRRPGKPDTYVHSIEEICRRIDETIAL
                     GGTGVLMQGGLHPDFNIEWYENLLSTLHAKYPAFQLHCFSPPEIHNIHLISGLDYETI
                     LRRLKAAGLYSMPGGGGEILDDEVRKRVSTKCSTEEWLDVMRAVHKVGLRSTATMMFG
                     IGDRIVHRVRHLERVRDVQDETGGFTAFIPWTFQRENTALGRKITEEPTGIDYLKMLA
                     VSRLFLDNVQHIQASWLTQGLRLGQTALRFGADDMGSIMIEENVVSAAGASAQANEME
                     LRYQISEAGFTPQQRDILYEYVSRENVARLDARKSMPLKQLSVAFAD"
     gene            complement(4136..>4879)
                     /locus_tag="VGB68_11045"
     CDS             complement(4136..>4879)
                     /locus_tag="VGB68_11045"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HEY0049816.1"
                     /translation="WSFLYGAMRGRFEMILDTAPARSAELLSEARVDAALVPVIAYQM
                     IEDVLLVPDICVGAREQVRSVCLVTRGEDLKTVKTVSLDVSSKTSVALAKIIFREFLA
                     SEPEWKISKPDLEKMLAESDCALLIGDPALTIDESVYRKFDLAETWKSFTGFGFVFAM
                     WMANAEKAEIARRIDLAAARDEGLRHLDEIISNYESEISLGREDFKKYLSENISYSID
                     DSMRKGLELYFRLAHKNGLINKYKPLSFI"
BASE COUNT         1233 a         1252 c         1184 g         1210 t
ORIGIN      
        1 gaaatcttcg attttcgaag ctttcttcgc ttcgtgttct ttcagattga actcttttaa
       61 attccacgct aatccgagtt tttccagcgc cttgatttgc agccagttga catcgaattc
      121 ataccaggtc aagccgtgac gcacggaagt cgggttggcg tgatgattat tgtgccagcc
      181 ttcgccgaaa ctgacggcgg cgaccagcgc gttgttggtc gaatcgtctt tcgtctcaaa
      241 acggcgcgag ccccagacgt gcgtgaccga attgacaaac cacgtgaaat gccagcccag
      301 cgtgaccggc agaaaaacgc cccacagaac catcgaccag ccgccgacga aacccaggat
      361 gacgccgagc gtaatcgtcg gcacatacca gtaattcgaa agcgtgacga gaaatttatc
      421 tttcagcaaa tcgggaataa aacgtctttc gacttcgacc gggttgccct gcgacgtgcc
      481 gaccgtcacc cagcccatat gcgcccagta agttccgtga cgcgggctgt gcgggtcttt
      541 gtccgtatcg caaaaggcgt gatggacgcg gtgcgtcgtc acccaatcgt tcgggctgcc
      601 ctgcaaagcc atcgagccga aaacggcgag aatatgtttg agccaatccg gcgtttcaaa
      661 accgcgatgc gtcaattgac ggtgaaatcc gacaccgatg ccgaggctgc ccgccagcca
      721 ccaggcaact aatccgaccc ctaaatttgt ccagctaaac gtaaatagtg cccagaccgc
      781 catgacgtga aagatggcga tgggaacaat tgtcacccaa tttgtttttc cgctttttct
      841 ttgttcgacc gttgctactt tttccacttt tttaaaaagc ccccttgtta ctgctaaaaa
      901 tttatgggag aaaaacaaaa acgcctttct caaacctatg atgaaaagcg tagcagattt
      961 attggctttt gattgtaaga gttttgtaat acacggattt acgcggcaca atttattttt
     1021 aagcggcgga aatacggcag aaatacgtaa attgagctat attataatta tgcaatacag
     1081 tcctttagca ataaaacgac gctacattct gggaaacctc gccgataagt gtgacgtgat
     1141 aaataaaatt ccggtttatc tgaacgataa atcgggcgag cccatcggat ttgccgacga
     1201 aagtatgggg cgttatgccg acgctttttt atttcattta cctgaagacg tctgtaaaaa
     1261 actttcgacg aacgggtacg agctggagat tgattacgac gtttccaaag aggacggcaa
     1321 cgccggaaaa agcgagcgtc tgacgctgaa ttatattatt cttacttcaa aggcgataaa
     1381 ctccgcaatc ccgcgccgtc atcctttcac caaagcggct gagtgagata cggacaattc
     1441 cgcggttttg tgcaggacgc gcttcaggag gcgcgtttta attccgctgg tgcttttttc
     1501 gtttcaaaac ggcggatttc tttcgcccag ccgagttttt cgaaaaggct aatcgtcagc
     1561 cagttcatat cgaattcata ccatttcaat ccgtgtcgcg ccgaacgcgg ctgggcgtga
     1621 tggttgttgt gccagccttc gccgaaagtc aggagcgcga ttatccagtt attagtcgaa
     1681 tcgtctttcg tttcaaagcg ccgctcgccc caaaaatgcg tcgccgaatt aaccagccag
     1741 gtcgaatgcc agccgaaaac gacgcgcgcg aaaacgcccc acaaaaccat cgaccagccg
     1801 ccggcggcaa acaaaattac gattgacgca agcagcggaa cgtaataaaa acgcgccagc
     1861 aaaacgtgaa atttgtcttt gagcaaatcc ggcacatagc gtttcaaggt ctcgcggtcg
     1921 tggtcttgcg ccgttccttt cagaatccat tccatatgcg cccaccagaa gcccggacgc
     1981 gtcgaatgcg ggtctttgtc ggtttcggta aattgatgat gaatgcggtg cgtcgccacc
     2041 catttcggcg cgtcgtcctg tattgccagc gcgccgaata tcgtcagcgt gtattcgagc
     2101 catccgggcg ctttaaagct gcgatgcgtc aaaaggcgat gatagcccaa gccgacgcct
     2161 aagcttccga caatccagtt tccgaccagc attaccgcca gattttccca gctgaacgta
     2221 aacaaagccg caatcgctaa aacgtgaaag gtgatgacga ttgataaatt tttccagttg
     2281 atgtttttgc tcagagattc cgattcgatg atgttttgca tttcttcccc tatgtaataa
     2341 aaattgcagc tttcggcgct tgaatttaga gtagcagatt agaggcaaag caatcgtaat
     2401 agttatgtaa tgaagcgtgc attgataaca aaaacgccgg ataatatatt atccggcgtt
     2461 cgcgggttta ttttttatcc gattgttttg tttaatcggc gaaagcgacg ctcaattgtt
     2521 tcaaaggcat agattttctc gcgtccagtc gggcgacgtt ttcgcggctc acgtattcat
     2581 acaaaatatc gcgctgctgc ggagtgaatc cggcttcgga gatttgatag cgcagttcca
     2641 tttcgttcgc ctgcgcgctt gcgcctgccg ccgagacgac gttttcttca atcataatcg
     2701 aacccatatc gtcagcgccg aagcgcagag cggtttgtcc gagacgcaag ccctgcgtca
     2761 gccacgaagc ttgaatgtgc tggacgttat ccagaaacag gcgcgatacg gcgagcattt
     2821 tcaggtaatc gatgccggtc ggctcttccg tgattttgcg cccgagcgcg gtgttttcgc
     2881 gctggaaagt ccagggaata aacgccgtaa agccgcccgt ctcgtcctgc acgtcgcgaa
     2941 ctctttcgag atggcgaacg cggtgaacta tacggtcgcc gatgccgaac atcatcgtcg
     3001 ccgtcgagcg caagccgact ttgtgaactg cgcgcatcac gtcgagccat tcctcggtgc
     3061 tgcatttggt cgaaactctt tttctgactt cgtcgtccag aatttcgccg ccgccgcccg
     3121 gcatcgaata aagtccggcg gctttcagac ggcgcagaat cgtctcgtaa tccagaccgg
     3181 aaataaggtg aatattgtga atttccggcg gcgaaaaaca atgcagttga aacgccgggt
     3241 atttggcgtg cagggtcgaa agcagatttt cataccattc gatattaaaa tcgggatgca
     3301 gtccgccctg catcagaacg ccggttccgc cgagcgcgat ggtctcgtcg atgcgcctgc
     3361 agatttcttc gatcgagtga acgtacgtgt cgggctttcc cggtcggcga taaaacgcgc
     3421 agaacgtgca gacgacgttg cagacattgg tgtaattgat attgcggtcg atgatatagg
     3481 tcacgacgtc ggtcggattt tttcgcatcc gaatctcgtg cgccgccaag ccgatgcgta
     3541 caaaatcgtt cgattcgagc agcgccgcgg cgtcttccgc cgtcaatctt tcgcctgcca
     3601 gagccttatc taaaatcggt tgaatattgc tgttcataaa tcaaaataat tttaacacgg
     3661 aaaactttag ccgcagagga cacagagaaa tgaaaccgca gatttacaca gataaacgca
     3721 gattttaaca ggatggacag gatgcacagg ataaaaaaat aaagctcaac gttccggttt
     3781 tgaattatct tgtaaatcct gtctatcctg ttaaaaattc ctctgtgaac tctgtgtcct
     3841 ctgtggctaa attctctcat tttgccaatc aaaatcgcac tttgccaaaa attttatgcg
     3901 aaatgaatct gaaatcgcgc aaaaagcgaa tctcaaatca gtctatagaa ttggtatcaa
     3961 gatttgtgcc ggacgattga tactttcggc gaacgaaatt cgccattacc ggcagaagag
     4021 aattttttac aaaggggaat gttgtttatg cgaaacaggg aaaacgaagg attcagagca
     4081 aacgacgcgg cgctgctttt atttatggtc gtcgccgtct ggctctgggc atttttcaga
     4141 taaacgaaag cggcttgtat ttattaatca agccgttttt gtgtgccagc cgaaaataaa
     4201 gctccaagcc tttccgcatc gaatcgtcaa tcgaatacga aatattttcc gacagatatt
     4261 ttttgaaatc ttcgcgcccg agcgaaatct cgctttcgta gtttgagata atctcgtcca
     4321 gatgccgcaa gccttcgtcg cgcgccgccg ccaaatcaat tctgcgcgca atttccgctt
     4381 tctccgcgtt tgccatccac atcgcaaaga caaaaccgaa tccggtaaag cttttccacg
     4441 tttccgccaa atcgaatttt cgatagacgc tctcgtcgat ggtcagcgcc gggtcgccga
     4501 taagcagcgc gcagtccgat tcggcaagca ttttctctaa atccggtttt gaaattttcc
     4561 attccggctc gctcgccaga aattcccgaa aaattatttt cgccagcgcg accgaagttt
     4621 ttgacgaaac gtcgagcgaa accgttttga ccgttttcaa atcctcgccg cgcgtcacca
     4681 gacagacgct tcggacctgc tcgcgcgcgc cgacgcagat gtccggaacg agcaggacat
     4741 cttcaatcat ctggtaagcg atgaccggga cgagcgcggc atcgacgcga gcctcagata
     4801 aaagctcggc tgagcgcgcc ggcgcggtgt cgagaatcat ctcgaatctg ccgcgcatcg
     4861 cgccgtagag aaacgacca
//
LOCUS       DASWNE010000557         4342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_88618, whole genome shotgun sequence.
ACCESSION   DASWNE010000557 DASWNE010000000
VERSION     DASWNE010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88618"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="VGB68_11050"
     CDS             <1..1006
                     /locus_tag="VGB68_11050"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEY0049817.1"
                     /translation="VVQTSNGSWRFDVARTPEDKTPFLQMRSWMMEDTARKIFTQAGK
                     NIDQLRAQANSRDFQPVKFGLRAGINLKSELKRLDSNNVVGFWQGKDKRLKDEYVVYT
                     AHWDHLGVGIPNAKGDTIYNGALDNASGVAQVLALAEAFTKLPPGEQPKRSQVFLFTT
                     AEEQGLLGAEWYAAHPIFSPAKTAANINIDGGNFYGLTTDYGALGAERSTLQSIVNDV
                     LRSRGMKFSPDTRPEQGYFFRSDHFPMAKAGIPALSIRNGTEYVGKPKDWSEKFFAEF
                     NREHYHQPSDEFRDDWRYDGMVQMLDINLAIGLRASNEPTLPRYNPTDEFARAQPNRK
                     "
     gene            complement(1181..2518)
                     /locus_tag="VGB68_11055"
     CDS             complement(1181..2518)
                     /locus_tag="VGB68_11055"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0049818.1"
                     /translation="MKAKTEKPRVVIIGGGFGGLQAAKALANKPVEVTLIDRKNHHTF
                     QPLLYQVATAVLSPGEIASPIRRILYRYKNVEVVLGEVTDFDKENQVVKLHDSSQIPY
                     DYLIVAAGARHSYFGHDEWEDDAPGLKTVEDAVEIRRRVLLAFELAERDALLTGTHEP
                     LIFVVVGGGPTGVELAGAIAGIARQALAKDFKLIDTTKARVMLFEGSQRVLGTFAEEL
                     SLKAKEQLEDLGVEVHLNSMVTAIEPGRIKVGDEWLECSVALWATGVAASPLGKKLGV
                     ETDRAGRVFVNPDLTIPNHKNVFVVGDMASIKQADGTPVPGVSPAAMQMAENAAANLL
                     RDLKSEPRKDFKYVDKGSMATIGRNKAIAEIQGWKFSGFIAWLMWLFLHVVFLIGFRN
                     RLAVLTEWMWAYFTRERSARLITGDAEEMHHAVRFIEARPEIQLVEKQKETVV"
     gene            2703..>4342
                     /gene="metG"
                     /locus_tag="VGB68_11060"
     CDS             2703..>4342
                     /gene="metG"
                     /locus_tag="VGB68_11060"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEY0049819.1"
                     /translation="MKTFYITTPIYYANSLPHLGHLYTTTVSDILIKHKKQRGFDTYF
                     LTGTDEHGVNIQRVAENGGKTPKQHVDFISGEMKKMFARFGLDNYDVFMRTTEPFHYE
                     AVQNLWREIAANKTPKGNETIYKGFYEAWFCPNCAEFKTENEYELRAGEDIPRCLIHE
                     RPLDRVSEESYFFRLSDYGETLLRIIEENPQRLRPEARRNEVVAFIKQGLQDLSISRE
                     KKSVSWGVPVPGDENHVMYVWLDALSNYITAIGYGNEERKSVGFEKYWQNVTHLVGKD
                     ILRFHTVYWWSFLLAAGIKLPDTVYAHGMWLDAEGRKMGKTLGNVVELDTLHKHFPTD
                     LIRYFVLREMVFGQDGKFGYENLIDRANADLASGLGNLSSRTLSMITKYRDGLIPGGK
                     ISEENYIFAKRAGINADEQDLVSVLDHARDEFIRKFDNYEFHQALEIVWSVIARIDKM
                     ISDAKPWELVKDANQAETLNAVLYRATETLRWLCVLLYPVMPEATRKIYAQIGLSENP
                     EQINPENLTWGELRAGTSIGETQAVFPRLDKNKIMSEI"
BASE COUNT         1088 a         1108 c         1141 g         1005 t
ORIGIN      
        1 cgtcgtgcag acttccaacg gctcttggcg ttttgacgtg gcgcgaacgc cggaagataa
       61 aacgcctttt ctgcaaatgc gttcgtggat gatggaagac acggcgcgaa aaatttttac
      121 gcaagccgga aaaaatatag accaattgcg cgcgcaagct aactccagag attttcagcc
      181 ggtcaaattc ggtttgcgcg ccggtattaa tctgaaaagc gaattaaaac ggctcgattc
      241 aaacaatgtg gtcggtttct ggcagggaaa ggacaagcgt ctgaaggacg aatacgtagt
      301 ttacacggcg cattgggatc acctcggcgt cggcattccg aacgcgaaag gcgacacgat
      361 ttacaacggc gcgctcgaca atgcttccgg cgtggcacag gttttggctt tggcggaagc
      421 ttttacgaaa ctgccgccgg gcgaacagcc gaaacgctcg caggtttttc ttttcacaac
      481 tgccgaagaa cagggacttt taggcgcgga atggtacgcg gcgcacccga ttttttcgcc
      541 cgcgaaaacg gcggcgaaca tcaatatcga cggcggaaat ttttacggct tgacgacgga
      601 ttacggcgcg ctcggcgcgg aacgctcgac gctgcaatcg atcgtgaacg acgttttgcg
      661 ctcgcgcgga atgaaatttt cgcccgatac gcgacccgag cagggctatt tcttccgttc
      721 cgaccatttt ccgatggcta aagccggcat tccggctctg agcatccgca acggtacgga
      781 atacgtcggc aaaccgaagg attggagcga aaagtttttc gccgagttta accgcgagca
      841 ttatcaccag ccgtcggacg agtttcgcga cgactggcga tacgacggaa tggtgcagat
      901 gctcgatata aacctggcaa tcggactgcg cgcatcgaac gagccgactt tgccgcgcta
      961 taatccgacg gacgaatttg cgcgggcgca gccgaatcgc aagtaaaaaa gattagccgc
     1021 caaaggacac aaaaaaacac gcaggtaaga atcaggaaaa tcagccgcga acgacacgga
     1081 cggacacgaa taaaagaaaa taaattaatt cgtattatgt gttattcgcg gctgattttc
     1141 cttttgtctt cttattgtgt cctttcgtcg atattttctt ttagaccacg gtttcctttt
     1201 gcttttcgac caactgaatt tccggtctgg cttctataaa tcttacggcg tgatgcattt
     1261 cctcggcgtc gcccgttatc aatctggcgc tgcgctcgcg cgtgaaatac gcccacatcc
     1321 actcggtcag aaccgccagc cgattgcgaa agccgattaa aaacacgacg tgcagaaaga
     1381 gccacatcag ccaggcaata aagccggaaa atttccagcc ctgaatttcg gcaatcgcct
     1441 tgttgcgccc gatggtcgcc atcgagcctt tatcgacgta tttaaaatct ttgcgcggct
     1501 cgctctttaa atcgcgcaga aggttggcgg cggcgttttc cgccatctgc atcgcggctg
     1561 gcgaaacgcc gggaacgggc gtgccgtcag cctgcttgat ggaagccata tcgccgacga
     1621 cgaaaacgtt tttgtggttc ggaatggtca aatcgggatt gacgaaaact cgcccggcgc
     1681 ggtcggtttc gacgccgagt tttttgccca gcggagaagc ggcaacgccc gtcgcccaga
     1741 gcgcgacgct gcattcgagc cattcgtcgc cgaccttgat gcgtcccggt tcgatcgccg
     1801 tgaccatgct gtttaaatga acttcgacgc ccaaatcttc cagttgttct ttcgctttca
     1861 gcgaaagctc ttcggcgaaa gtgcctaaaa cgcgttgact gccttcaaaa agcatcacgc
     1921 gcgccttggt cgtgtcaatt aatttaaaat cttttgccag cgcctgccgg gcgatgccgg
     1981 caatcgcgcc cgccagctcc acgccggtcg gaccgccgcc gacgacgacg aaaatcagcg
     2041 gctcgtgtgt tcccgtcaaa agcgcgtcgc gctcggcaag ctcaaaagcg agcaaaacgc
     2101 ggcggcgaat ctcgaccgcg tcttcgacgg ttttcaaacc gggcgcgtca tcttcccatt
     2161 cgtcgtgacc gaaatacgag tgacgcgcgc cggcggcgac aatcaaataa tcgtagggaa
     2221 tttgcgagct gtcgtgcaat tttacgacct gattttcctt atcgaaatcc gtgacttcgc
     2281 cgagaactac ctcgacgttt ttataacgat acaaaatgcg ccgaatcggc gaggcgattt
     2341 cgcccggaga aagcacggcg gtcgccacct gatagagcag cggctgaaaa gtatgatgat
     2401 ttttgcggtc gatcagggtc acttcgaccg gtttgttcgc cagagccttt gctgcctgca
     2461 aaccgccgaa gccgccgccg atgattacga ctctcggttt ttctgttttc gctttcatta
     2521 ctttctcctt actatataaa gcattttatc attgtgtgaa ataattcaca atatttattt
     2581 acgacgttat tatgataaaa agttgcaggt tttttcgaaa aaattcagtt cgttaggaaa
     2641 caatcaaaag cgcgcgattt gtcgaaacgg acaaacgcct tataattaag ctttttattg
     2701 cgatgaaaac tttttatatt acaacgccga tttattacgc caacagcctg ccgcacctcg
     2761 gacatcttta tactacgacg gtttccgata ttttgataaa acacaaaaag cagcgcggat
     2821 tcgacacgta ttttctgacc ggaacggacg aacacggcgt aaacattcag cgcgttgccg
     2881 aaaacggcgg caaaacgccg aaacagcacg tcgattttat ctcgggcgag atgaaaaaaa
     2941 tgtttgcccg tttcggcttg gacaattacg acgtttttat gcggacgacc gagccgtttc
     3001 attacgaagc ggtgcagaat ctatggcgcg aaatagctgc aaacaaaacg ccgaaaggca
     3061 acgaaacgat ttacaaaggt ttttacgaag cgtggttttg cccgaactgc gccgagttta
     3121 aaaccgaaaa cgaatacgag ctgcgcgccg gcgaagacat tccgcgctgt ctgatacacg
     3181 agcgcccgct cgaccgcgtt tcggaggaaa gctatttttt ccggctttcc gattacggcg
     3241 aaacgctttt acgaatcatc gaggaaaatc cgcagcggct tcgacccgaa gcgcggcgca
     3301 acgaggtcgt tgcttttatc aaacagggct tgcaggattt atcgatcagc cgcgagaaaa
     3361 aatccgtcag ttggggcgtt cccgtgccgg gcgacgaaaa tcacgtgatg tatgtctggc
     3421 tcgacgcgct ttccaattac atcacggcaa tcggttacgg caacgaggaa agaaaatccg
     3481 tcggatttga aaaatactgg caaaacgtga cgcacctggt cggcaaagac attctgcgtt
     3541 ttcacacggt ttactggtgg tcgtttctgc tggcggcggg cataaagctg cctgatacgg
     3601 tttacgcgca cggaatgtgg ctcgacgccg aaggtcgcaa aatgggcaaa acgctcggca
     3661 acgtcgtcga gctcgacact ttgcacaaac attttccgac ggatttaatc agatatttcg
     3721 ttttgcgcga gatggttttc gggcaggacg gcaaattcgg ctacgaaaat ctgattgacc
     3781 gcgccaacgc ggatttggcg agcggtctgg gaaatttgtc gagccgcacg ctctcgatga
     3841 ttacgaaata ccgcgacggc ttgattccgg gcggaaaaat cagcgaggaa aattatattt
     3901 tcgccaagcg cgccggcatc aacgcggacg agcaggacct cgtttccgtg ctcgatcacg
     3961 cgcgcgacga gtttatacga aagtttgata attacgagtt tcatcaggct ttggaaatcg
     4021 tctggtcggt gattgcgcgg attgacaaga tgatttccga cgccaagccg tgggagctcg
     4081 tcaaggacgc gaatcaggcg gaaactttga acgcggtctt gtatcgggcg accgaaactc
     4141 tgcgctggct gtgcgttctg ctttatccgg taatgccgga agcgacgcgt aaaatctacg
     4201 cgcaaatcgg tttaagcgaa aatcctgagc aaataaatcc tgaaaattta acctggggag
     4261 aattgcgggc gggaacgagt atcggtgaaa ctcaagccgt ttttccgcgt ctggataaaa
     4321 ataaaattat gagcgagatt ga
//
LOCUS       DASWNE010000558         4435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_266825, whole genome shotgun sequence.
ACCESSION   DASWNE010000558 DASWNE010000000
VERSION     DASWNE010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4435
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266825"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VGB68_11065"
     CDS             <1..684
                     /locus_tag="VGB68_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049820.1"
                     /translation="AFDENGLLPTTVLKVGNEIWMYYVGYQLGMKVRYYQFQGLAISK
                     DGGESFERRLKVPVIDRSDAEMLNRTSAHVRHDGEKFQMWYVGGSDWTESGDKYLPVY
                     NIKYLESPDGINWGDEGTVAIDFKDADEHALGKPFVIYAEGKYRMFFSSRTYSKKAYR
                     IGYAESDDCLNWTRRDEEVGIDVSASGWDSESMSYASVIQYKDRYYMFYNGNNLGETG
                     FGYAILEQW"
     gene            678..1658
                     /locus_tag="VGB68_11070"
     CDS             678..1658
                     /locus_tag="VGB68_11070"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0049821.1"
                     /translation="MVETATIEPAEEKAAIRAERYSGERGAEWNEFVKNSKNGTFLFD
                     RGYMDYHADRFTDCSPMFRDAGGKLIAVMPANLKDDALVSHGGLTFGGVVSNQRMKTP
                     SMLEIFESLIKFAKDAGAARIVYKAVPHIYHAQPAEEDLYALFRHNARLIKREASTTI
                     NLKERLPVSKGRKWTINQSRKNKVEVRESVDFETFMRLEAEVLAAKYNVVPVHTAAEM
                     HLLAERFPENIRLFGAFFNDEMLAGAIVYASETVAHAQYMAANAEGKQLCALDAILQE
                     LIAARYAGKRYFDFGISTEKQGRYLNVGLVEYKESWGGRATVYDTYEIVF"
     gene            1803..2225
                     /locus_tag="VGB68_11075"
     CDS             1803..2225
                     /locus_tag="VGB68_11075"
                     /inference="COORDINATES: protein motif:HMM:NF017345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HEY0049822.1"
                     /translation="MESLIEQCRLIDLPVITDPRGNLTFIECNRHIPFEIKRVYYLYD
                     VPGGAERAGHGHKKLEQLVIAMSGSFDIVLDDGFQKKRFHLNRSYQGLYVCPMIWRDI
                     DNFSGGSVCMVLASDFYDEDDYFRHYPDFLRAAREEKA"
     gene            2222..3367
                     /locus_tag="VGB68_11080"
     CDS             2222..3367
                     /locus_tag="VGB68_11080"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY0049823.1"
                     /translation="MRVPFLDLNSVYSEIKNELDEAYARVMSSGWYILGAEVEAFENE
                     FAEYCQAKHCIGVANGLDALHLILRGCRIGEGDEVIVPSNTYIATWLAVSYAGATPVA
                     VEPDIETYNLDPQKIEAAITPKTRAIMPVHLYGQPADMDAINEIARRYNLKVIEDAAQ
                     AQGARYKNEPTGTLSDAAGFSFYPGKNLGAFGDAGAIVTNDDELADAMRVLRNYGSRV
                     KYYNEVKGFNSRLDPLQAAFLSVKLRHLDEWNARRQTIARKYIENLSGCSKIVLPLLE
                     EEAESVWHVFVIQCAERDALQDFLKGRGVETLIHYPVPPHLSQAYGDDFAEKPDLPIA
                     EKLAETVLSIPISPHLTESEQDFTIETIKEFDRLESSKHFYEHRTAG"
     gene            3345..4322
                     /locus_tag="VGB68_11085"
     CDS             3345..4322
                     /locus_tag="VGB68_11085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0049824.1"
                     /translation="MSTAPLVSICVPSYNHAQYLPATLDSALAQTYPNIEIIVLDDGS
                     KDDSLKIAGDYQARFPDRVKVYTHAGGANLGISRTVNAAFAHAKGKYWMGLPSDDILY
                     PDKIEKQVAFLEKNPGLGFVYCYVNYIDSEGKPVSGSFGRDITADADPLKSMILENFI
                     PGMAVLARREAIARVGDHDADLIYSDWDFWVRLFSMYKGGFIPESLIGYRVHDYNTSV
                     GTPRATQIGHIRDFYLKLLQHVDNGAIDKSYRKIIENQIENLPARHASWLMIDCYEAL
                     GEGRRKAAYRALKDAFHVSPRFVLSGQRVGVVLKKTLLSLIPRSKMKQN"
BASE COUNT         1104 a         1122 c         1210 g          999 t
ORIGIN      
        1 gcatttgacg aaaacgggct tttgccgacg accgtcttga aagtcggcaa cgaaatctgg
       61 atgtattacg tcggctacca gctcggaatg aaggttcgct attaccagtt tcagggcttg
      121 gcgataagca aagacggcgg cgagagcttc gagcggcgct tgaaagttcc ggtcatcgac
      181 cgcagcgacg cggaaatgct caaccgcacg tcggcgcacg tccgccacga cggcgaaaaa
      241 tttcagatgt ggtacgtcgg cggcagcgac tggaccgagt cgggcgataa atatctgccc
      301 gtttataata ttaaatatct ggaatcgccg gacggcatta attggggcga cgaaggcact
      361 gtcgccattg attttaaaga cgccgacgaa cacgcgctcg gcaagccttt cgtcatttac
      421 gccgaaggca aatatcggat gtttttctcg tcgcgcactt attcgaaaaa agcttatcgc
      481 atcggctacg ccgaatccga cgattgcctg aactggacgc gccgggacga ggaagtcggc
      541 atcgacgttt ccgcgtcggg ctgggattcc gagtcgatgt cctacgcgag cgtgattcaa
      601 tacaaagacc gctattatat gttttataac ggcaataatc tgggcgagac gggtttcggc
      661 tacgcgattc tggaacaatg gtagagacgg cgacaatcga gccggcggaa gaaaaagcgg
      721 cgattcgcgc cgaaagatat tcgggcgagc gcggcgcgga gtggaacgag ttcgtcaaaa
      781 attcgaaaaa cggcacgttt cttttcgacc gcggttatat ggattatcac gccgaccgct
      841 tcacggattg ctcgccgatg tttcgcgacg ccggaggcaa attaatcgcc gtgatgccag
      901 ccaatttaaa agacgacgcg ctcgtctcgc acggcggctt gacgtttggc ggcgtcgttt
      961 cgaatcagcg tatgaaaacg ccgtcgatgc tcgaaatatt tgaaagctta atcaaatttg
     1021 ccaaagatgc gggagccgca cgcatcgttt acaaagccgt tccgcatatt tatcacgcgc
     1081 agccggcgga agaagattta tacgcattgt ttcgccacaa cgcgcggctg attaaacgcg
     1141 aagcttcgac gaccatcaat ttaaaggagc gtctgccggt cagcaaaggg cgcaagtgga
     1201 caatcaatca gagccgtaaa aacaaggtcg aggtgcgcga aagcgtcgat ttcgaaacgt
     1261 ttatgcggct cgaagccgaa gttctggcgg cgaaatacaa cgtcgttccg gttcacacgg
     1321 cggcggaaat gcatctgctg gcggaacgtt ttcccgaaaa catccggctc ttcggcgctt
     1381 tttttaatga cgagatgctg gcgggcgcaa tcgtctacgc cagcgaaacg gtcgcgcacg
     1441 cgcagtatat ggcggcgaac gcggaaggca agcagctatg cgcgctggac gcgattttgc
     1501 aggaattgat tgccgcccgc tacgccggaa aacgatattt cgatttcggc atctcgaccg
     1561 aaaagcaggg gcgatattta aacgtcgggc tggtcgaata caaggaaagc tggggcgggc
     1621 gcgcgaccgt ctatgatact tacgaaatag ttttttgatt gaaagccgtt tttatctttg
     1681 ccaaaacctt cgcgcccgcg cgcggttaat aaaaattttt aaagcaaaga gcgcgaagaa
     1741 aaagacgcga aaaaacgcgg agtgattttc gagccggctt tcgttaatct ggtaattaag
     1801 ctatggaatc gctgattgaa caatgtcgat tgattgattt gccggtcatt accgacccgc
     1861 gcgggaatct gacttttatc gagtgcaacc ggcacatacc gtttgaaatc aagcgcgttt
     1921 attatctgta cgacgtgccg ggcggagccg agcgagccgg tcacgggcat aaaaaactgg
     1981 agcagctcgt cattgccatg tcgggcagct tcgacatcgt gctcgacgac ggctttcaga
     2041 aaaagcggtt tcatctgaat cgttcttatc agggacttta cgtctgcccg atgatttggc
     2101 gcgacatcga caatttttcc ggcggctcgg tctgtatggt gctggcttcg gatttctacg
     2161 acgaggacga ttattttcgt cattatcccg attttctgcg ggcggcgcgg gaggaaaaag
     2221 cgtgagagtt ccgtttctcg atttaaactc cgtctattcg gaaatcaaaa acgaactgga
     2281 cgaagcctac gcgcgcgtga tgagctcggg ctggtatatt ctgggcgcgg aagtcgaagc
     2341 ttttgaaaac gaatttgccg aatattgtca ggcaaaacac tgcatcggcg tcgccaacgg
     2401 actggacgcc ctgcatctga ttctgcgcgg ctgccggatt ggcgaaggcg acgaagttat
     2461 cgttccgtca aacacttata tcgccacgtg gctcgccgtt tcatacgccg gagcgacgcc
     2521 cgtcgcggtc gagcccgaca tcgaaactta caatctcgac ccgcagaaaa tcgaagcggc
     2581 aatcacgccg aagacgcgcg cgataatgcc ggttcatctt tacggtcagc ccgccgatat
     2641 ggacgcgata aacgaaatcg cgcgccggta caatctgaaa gtcatcgaag acgcggcgca
     2701 ggcgcagggc gcgcgttata aaaacgaacc gacgggcaca ttgtccgacg ccgccggatt
     2761 cagcttttat ccgggcaaaa atctcggcgc tttcggcgac gccggagcaa tcgtgacgaa
     2821 tgacgacgaa ttggcggacg cgatgagagt tttgcgaaat tacggctcgc gcgtgaaata
     2881 ttacaacgaa gtgaaaggct ttaattcgcg tctcgacccg ctgcaagccg cttttctgtc
     2941 ggtcaagctc cggcatctcg acgaatggaa cgcgcggcgg caaaccattg cgcgaaagta
     3001 tatcgaaaat ttgtccggct gctcgaaaat cgttttgccg cttctggaag aagaagccga
     3061 atcggtctgg cacgtgtttg tcattcaatg cgccgaacgc gacgcgctgc aagattttct
     3121 gaaagggcgc ggcgtcgaaa ctctgattca ctatccggtt ccgccgcacc tttcacaggc
     3181 ttacggcgac gactttgcgg aaaaaccgga tttgccgatt gccgaaaaat tagccgagac
     3241 cgttttgagc attcctatca gccctcattt gaccgaatcg gagcaggatt ttacgataga
     3301 aactataaaa gaattcgacc gcctggagtc gtcaaagcat ttttatgagc accgcaccgc
     3361 tggttagcat ctgcgttccc tcttacaatc acgcgcaata tttgccggcg acgctcgaca
     3421 gcgcgctggc gcagacttac ccgaacatcg aaattattgt tctggacgac ggctcgaaag
     3481 acgatagttt aaagattgcc ggagattacc aagcccgctt tcccgaccgc gtaaaagtct
     3541 acacgcacgc gggcggcgcg aatctcggaa tttcccgcac cgtcaacgcc gctttcgccc
     3601 acgcgaaagg taaatactgg atgggtttgc cttccgacga cattctgtat cccgacaaaa
     3661 tcgaaaaaca ggtcgccttt ctggaaaaaa accccgggct cggcttcgtt tattgctacg
     3721 tcaattacat tgattccgaa ggaaagccgg tttccggaag tttcggcaga gacattaccg
     3781 ccgacgccga cccgctcaaa tctatgattc tggaaaattt cattcccgga atggcggttc
     3841 tggcgcggcg cgaggcgatc gcgcgcgtcg gcgaccacga cgccgattta atctacagcg
     3901 actgggattt ctgggtgcgg cttttttcga tgtataaagg cggttttatt cccgaatcgc
     3961 tcatcggtta tcgcgttcac gattataaca ccagcgtcgg cacgccgcgc gcgacgcaaa
     4021 tcggtcatat tcgtgatttc tatcttaaat tattacagca cgtcgataac ggagcgatag
     4081 acaaatctta tcggaaaatt atcgaaaacc agatagagaa tttaccggcg cgccacgcga
     4141 gctggctgat gattgattgt tacgaggcgc tcggcgaagg gcggcgcaag gcggcgtacc
     4201 gcgctttaaa agacgctttt cacgtttcgc cgcgattcgt tttgagcggg cagagggtcg
     4261 gcgtggtgct gaaaaaaacg cttttaagtt tgataccgcg ctcaaaaatg aaacagaatt
     4321 aggttagact ggaaaattat cgcccgttgg ttcgataaat ttttgccggc aaaactggta
     4381 tacttaaaaa ggctttattt tagcttccat tatgaaagaa aaaaactcac tcccg
//
LOCUS       DASWNE010000559         5205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_19220, whole genome shotgun sequence.
ACCESSION   DASWNE010000559 DASWNE010000000
VERSION     DASWNE010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19220"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VGB68_11090"
     CDS             complement(<1..386)
                     /locus_tag="VGB68_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049825.1"
                     /translation="MKKLESKLFCIIAILLTTAASLAAQNRFEGYSLTVEADESGTCP
                     VKYKPSGSNYVDVLFAGTKRIASDVTACPGSGTTVNLGRVTPNGDGRWCFQGNNDYYD
                     VQLVGAVKYLWHPTTKNTGFYNIKDFR"
     gene            827..2308
                     /locus_tag="VGB68_11095"
     CDS             827..2308
                     /locus_tag="VGB68_11095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase"
                     /protein_id="HEY0049826.1"
                     /translation="MKIVVIGSGFGGLAAAIRLQARGHEVVIVEKRDKAGGRAYVYEQ
                     DGFTFDGGPTIITAPWLIDELFELAGKKTSDYIELVKLNPFYNIRFEDGTVFNYNDDR
                     AELLDQIRKFDADGVDGYERFARDLGDIYRAGFELIDQPFSRWQDMLRVLPTMIRLRS
                     DRSVYKFASRYFKDERLRQAFSFHPLLIGGNPFTATSIYAMIHRLEQEYGVWFAKGGT
                     GAIVRALCNLFMDLGGEIYLQAEVAEIYINRAREAGGVRLKTDEFFQADAVVSNADVA
                     FTYLNLIKSDFRRKYTDRKIHSMTYSMSLFVYYFGTDKKYENMAHHEIIMGARYKGLL
                     DDIFNKKILAEDFSLYLHRPTATDPALAPDGCDSWYVLSPVPHLQGKTDWKREAGDYR
                     DRIVKYLEERYMPDLSKHIVTEHYIDPLHFQNDLNSYLGSAFSVEPTLMQSAFFRPHN
                     RSEDVANLYFVGAGTHPGAGLPGVISSGKIVANLIGAADKITN"
     gene            2446..3204
                     /locus_tag="VGB68_11100"
     CDS             2446..3204
                     /locus_tag="VGB68_11100"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HEY0049827.1"
                     /translation="MWYWTKVLFLVLVGAVVVWAGYEFFTFPNISKLRSENPATTSMI
                     EYRISEAKANGAEPRKYQIWMPIAQISPNLQKAVLAGEDTHFFAHKGFDWDAIQKAWD
                     EAQKEAEKEAKEEGDYDPDAWIPPLPSFKRGASTITQQLAKNLYLSEERSFLRKGREA
                     AITYFLERELSKKRILEIYLNVIEWGDGIYGAEAAARTYFKKSASDLTPSEAAYLSAM
                     IPSPLNVFNPNKNKKRVVRRQKVILRGMNYVKLQ"
     gene            complement(3293..4609)
                     /locus_tag="VGB68_11105"
     CDS             complement(3293..4609)
                     /locus_tag="VGB68_11105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY0049828.1"
                     /translation="MKILPEKNKQSGFSLLELMIVMVLMLIITATVFSLMRGTIITAN
                     ANYEMTDAGQGLRNSQEFLSRDILSAGDGLKGIANIWIPTAFTTKYLTTQSATVLDPT
                     DRGFISLGAVISDDNIPASTIIPDSNPAKNIRQRTDRITILAADPSFTPHSIPANWTN
                     HNTGAIRIPGGDLSEFNAGEIYFISDGASATFGTITRKDSADWIYWENGDAYQLNRTG
                     YTGNLASATNLSRSPATLMRMQIIQFFVDEDGKLIRRVFGVKNAGYSDSVIAEHVVSL
                     QFRYILKPTGNGTLIFDQPVTQLADGQQTLVRMVEPTVVVETAYPLQDGEKYQLEGIT
                     QIGLRNIQFVEALIPRDSYGNTELPTGGPTPNITPTPTPTPSPTPTPTATPVPTATPV
                     PTATPFPTATPVPTATPVPTATPTAAPTATRTPTPVPTPTPGSGEG"
     gene            complement(4725..>5205)
                     /locus_tag="VGB68_11110"
     CDS             complement(4725..>5205)
                     /locus_tag="VGB68_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049829.1"
                     /translation="SKTWFDALSALYPEGSGDARNLTGEGYRMADENLPDLTQSTTVR
                     AGIIAGTNISSLTASPGRDADGLRRNGGVINFPRFLETWNLNGITQTWNYSGSFIPLY
                     RSTQALSNWENDTSVIYMPPRRNWSFDQTFLNPNQLPPGTPFFQYVSSTGFRQKLRD"
BASE COUNT         1214 a         1307 c         1406 g         1278 t
ORIGIN      
        1 cgaaagtcct tgatattata aaagccggtg tttttcgtcg tcggatgcca aagatatttg
       61 accgctccta cgagctgaac gtcataataa tcattgttgc cctgaaaaca ccagcgcccg
      121 tcgccgttcg gtgtgacacg ccccaaattc acggtcgtgc cgctgcccgg acaggctgtc
      181 acgtcagacg cgattctttt tgtgcccgcg aaaagaacgt cgacgtagtt gctgcccgaa
      241 ggcttgtatt tgaccgggca cgtcccgctc tcgtcggctt cgacagtcag cgaatagcct
      301 tcaaatcggt tttgggcggc aaggcttgcc gccgtcgtga gcaaaatcgc gataatacaa
      361 aatagtttcg attccagttt tttcattttc taatatcctt gaatttttgt cggcgctgcc
      421 ggcggagtta aattccgtca ggtttttata tttattgctt tccagatata tttattctgc
      481 ttcggtaaga ctcggacaga ttaaatgagg gcttccggca agacggcttc gaaacacttc
      541 gggcgagcga tgaaataaac tcagacccgt gtaatccgac cgatgcagca aagttacttt
      601 ttttgtcgcc gaaagtcaat attttccggt cttgtttacc tttagctgaa agaattcata
      661 taatcggttt aagctaaaaa ttaatcgccg ggctgatttt caaaatcttt ttccgcgcag
      721 gttaatcgca gcctgttgtc gatattggaa agccgacggc gagcgcgaga ttttacgaac
      781 ggtaaatgca cgcaatttcg tgctttcccg tggtttttat ttttttatga agatagtcgt
      841 aatcggaagc ggatttggcg gtttggcggc ggcgatacgc ctgcaggcgc ggggacacga
      901 ggtcgtcatc gtggaaaagc gcgataaagc gggcgggcgc gcctatgttt acgagcagga
      961 cggcttcacg tttgacggcg gaccgaccat tattacagcg ccgtggctga tcgacgaatt
     1021 atttgagctg gcgggcaaaa aaacctccga ttatatcgag ctggtcaagc tgaatccttt
     1081 ctataatatc cgttttgaag acggaacggt tttcaattac aacgacgacc gcgccgaact
     1141 gctcgaccag attcgcaaat tcgacgccga cggcgtcgac ggctacgagc gtttcgcgcg
     1201 cgatttaggc gatatttacc gcgccggttt cgagctgatc gaccagcctt tttcgcgctg
     1261 gcaggatatg ctgcgcgtac tgccgacaat gattcggctg cgctccgacc gctcggttta
     1321 caaattcgcc tcgcgttatt tcaaggacga aagactgcgg caagccttca gctttcaccc
     1381 gcttttaatc ggcggcaacc cgtttaccgc aacctcgatt tacgcgatga ttcatcgcct
     1441 ggagcaggaa tacggcgtct ggtttgccaa aggcggaacg ggcgccatcg tccgggcgct
     1501 gtgcaatctg tttatggatt tgggcggcga gatttacctg caagccgagg tcgccgaaat
     1561 ttacatcaac cgggcgcgcg aagccggcgg agtccgtctg aaaacggacg aattttttca
     1621 agccgatgcg gtcgtctcca atgcggacgt cgcttttaca tatttaaatt taattaaaag
     1681 cgattttcgg cgcaaatata ccgaccgtaa aatccattcg atgacttact cgatgtcgct
     1741 tttcgtctat tatttcggga cggacaaaaa atatgaaaat atggcgcatc acgaaatcat
     1801 aatgggcgcc agatacaaag gcttgctcga cgacatcttt aacaaaaaaa ttctggctga
     1861 agatttttcg ctctacctgc atcgcccgac ggcgaccgac ccggcgctcg cgcccgacgg
     1921 ctgcgattcg tggtatgtgc tgtcgcccgt tccgcatctg caaggcaaaa ccgactggaa
     1981 acgggaagcg ggcgattacc gcgaccggat tgtgaagtat ctggaagagc gctatatgcc
     2041 ggatttgtcg aaacacatcg tcaccgagca ttatatcgac ccgcttcatt ttcagaacga
     2101 tttgaacagc tatctcggca gcgccttttc cgtcgagccg acgctgatgc agtccgcatt
     2161 tttccgcccg cacaaccgga gcgaagacgt ggcgaatctt tatttcgtcg gcgcgggcac
     2221 gcatcccggc gcgggattgc cgggcgtgat cagctcggga aaaatcgtgg caaaccttat
     2281 cggcgctgcc gataaaatta cgaattaaaa attacgaatt acgaataaga atatttcgtt
     2341 aaaaccgcat ccgattcgcg aggttgaaaa ataagacgtt taaaacgatt atgaacttta
     2401 aagttttaca aatcgtaatt cgtaattgat aattcgtaat tgtttatgtg gtattggaca
     2461 aaagttttat ttctcgtact ggtcggcgcg gtcgtcgtct gggcgggtta cgaatttttt
     2521 acttttccga atatttccaa gcttcgcagc gaaaacccgg cgacgacttc gatgattgaa
     2581 tatcggattt cggaagcaaa ggcgaacggc gcggagccga gaaaatatca aatctggatg
     2641 cccatcgcgc agatttcgcc gaatctgcaa aaagccgttt tagcgggcga ggacacgcat
     2701 tttttcgcgc ataaaggctt cgattgggac gcgattcaaa aagcctggga cgaggcgcag
     2761 aaggaagccg aaaaggaagc caaggaagaa ggcgattacg acccggacgc gtggattccg
     2821 ccgctgcctt ctttcaaacg cggcgcgtcg acgattactc aacaactggc gaaaaatctt
     2881 tatttgtcgg aagaacgctc gtttttgcga aaagggcgcg aagccgccat cacttatttt
     2941 ctggagcgcg agctgtcgaa aaaacgcatt ctggaaattt atctgaacgt catcgaatgg
     3001 ggcgacggca tctacggcgc ggaagccgcc gcgcgaactt atttcaagaa atcggcttcg
     3061 gatttaacgc cgagcgaagc cgcatatctt tcggcgatga ttccgagccc gctcaacgtc
     3121 tttaacccga acaaaaacaa aaagcgcgtc gtccggcgac agaaggtcat cctgcgcggg
     3181 atgaattacg tcaagcttca ataacccaat taaaaattaa aaattacgaa ttacgaattg
     3241 agagaataaa tccatcattc gtaattcgta attcgtaatt cgtaattttc gcttagcctt
     3301 cgccgctgcc gggcgtcggc gtcggaacgg gtgtcggcgt tcgagtcgcc gtcggggcag
     3361 cggtcggcgt cgcggtcggc accggagttg ccgtcggaac cggcgtcgcg gtcgggaatg
     3421 gcgtcgcggt cggaacaggt gtcgcggtcg gaaccggagt tgccgtcggc gtcggcgtcg
     3481 gcgacggtgt gggcgtcggc gtcggcgtaa tattcggagt cggtccgccc gtcgggagtt
     3541 ccgtatttcc gtaactgtcg cgcggaatca gcgcttcgac aaactgtatg tttcgaagcc
     3601 cgatttgcgt aatgccttcg agctgatatt tttcgccgtc ctgaagcggg taagccgtct
     3661 cgacgacgac ggtcggctcg accattctga cgagcgtctg ctgaccgtcg gcaagctgcg
     3721 tcacgggctg gtcgaaaatc aaagttccgt tgccggtcgg tttcaaaatg taccggaatt
     3781 gcagcgaaac gacgtgttcg gcaatcacgc tgtcggaata tccggcattt ttgacgccga
     3841 aaacgcggcg tatcagcttg ccgtcttcgt cgacgaaaaa ctgaattatc tgcatccgca
     3901 taagcgtcgc cggacttcgg ctcagattcg tcgccgaagc gagattgccc gtgtagcccg
     3961 ttcgattcag ctggtaggcg tcgccgtttt cccagtaaat ccaatccgcc gaatctttgc
     4021 gcgtaatggt gccgaaagtc gcgctcgcgc cgtcggaaat aaaataaatt tcgcccgcgt
     4081 tgaattccga caaatcgccg cccggaatcc gaatcgcgcc cgtgttgtga tttgtccagt
     4141 ttgccggaat cgaatgcggc gtaaaactcg ggtcagccgc cagaatggta atgcggtcgg
     4201 ttcgctggcg aatatttttc gccggattcg aatcgggaat aatcgtcgaa gccggaatgt
     4261 tgtcgtcgga aatgaccgcg cccagactga taaagccgcg gtcggtcggg tctaaaacgg
     4321 tcgcgctctg cgtcgtcaga tatttggtcg taaaagcggt cggaatccat atattggcga
     4381 tgccttttaa accgtcgccc gccgagagaa tgtcgcgcga cagaaattcc tgagaattgc
     4441 gcagcccctg accggcgtcg gtcatctcgt agttggcgtt ggcggtgata atcgtgccgc
     4501 gcataagcga gaaaaccgtc gccgtgatga tgagcatcag caccatcaca atcatcaatt
     4561 ccagaaggga aaatccggac tgcttgtttt tttcaggtaa aattttcatt tctttttgtt
     4621 attcgtcctt tgtccattgt ctttcattgg ttgaagtaaa accgaaaact gaaattttac
     4681 ttcaaccggc taaaggctaa gaacgaagaa ccggcggcag ttgcctaatc gcgtaatttc
     4741 tgtcggaatc cggtcgacga gacgtattgg aagaaaggcg ttcccggcgg cagttgattc
     4801 ggattgagaa aggtctggtc gaagctccag tttcgtcgcg gcggcatata gattaccgac
     4861 gtgtcgtttt cccagtttga cagcgcctgc gtcgagcgat aaagcggaat aaacgagccg
     4921 gagtaattcc acgtctgcgt gatgccgttt aaattccagg tctccagaaa gcgcgggaaa
     4981 ttaatcacgc cgccgttgcg gcgcaagccg tcggcgtctc tgccgggcga agcggtcagg
     5041 ctggaaatat tcgttccggc gataattccg gctctgacgg tcgtgctttg cgtcaggtcg
     5101 ggcagatttt cgtccgccat ccggtagcct tcgccggtta agtttcgcgc gtcgccgctg
     5161 ccttccggat acagcgcgct gagcgcgtcg aaccacgttt tggaa
//
LOCUS       DASWNE010000560         1832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_654824, whole genome shotgun sequence.
ACCESSION   DASWNE010000560 DASWNE010000000
VERSION     DASWNE010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654824"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="VGB68_11115"
     CDS             complement(<1..734)
                     /locus_tag="VGB68_11115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0049830.1"
                     /translation="MSVSVLVPSYNHAPFVERTLRSIFAQTFPPKKLIVIDDGSKDES
                     AEIIRRVLRECPFENEFIARENRGLSATLNEGFAKTGSEFFAYLGSDDLWFPNFLEER
                     IRLLSRRPDAVLAFGHAFLIDENEQIIDSTANWTSFADGDMLPFLLRGQIFSSPSVVY
                     RRSALEKYEWNENSVLEDYELYLKLCADGEFARDERILCAWRQHGWNVSGDFPLMLSE
                     WIAAQNLTARNLNLSRAELDKIQTEL"
     gene            847..>1832
                     /locus_tag="VGB68_11120"
     CDS             847..>1832
                     /locus_tag="VGB68_11120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0049831.1"
                     /translation="MTNGKGRTTKILHTLDTLNRGGAEMQALDVCRNARKHGFEMTFV
                     AFGGGKLEEDFRASGVDFIQSQRRLPVDFNLIFKLRKIIKDRRIEIAQAYQPVEGLHL
                     YLAAIGLNVKKVLSFQGFIQDAKNRKTAKFLIPRMDANIVVSQGLKKWLERADNLDTG
                     KNFQVVYNGADRERIISNHPVLRAELNLSENASLFGMIANFYRDPRKDQLTVCRALPK
                     VFAEIENARCVFVGRTETGAERKFKECVDFCRENKIDDRVHFLGARNDIPDVLASLDV
                     FVLSSLHEGLPVAATEAMLVGVPMILSDIEPLLEVSAGGRYAEMFQTQNPDEL"
BASE COUNT          469 a          484 c          452 g          427 t
ORIGIN      
        1 tttagttcgg tttgaatttt atccagctcc gcccgcgata aattcagatt ccgcgccgtc
       61 aggttctgcg ccgcaatcca ttccgagagc atcagcggaa aatcgccgct cacattccag
      121 ccgtgctgtc gccacgcgca caaaatccgc tcgtcgcgcg caaattcgcc gtcggcgcaa
      181 agttttaagt atagttcgta atcttccaaa acggaattct cgttccactc atatttttcg
      241 agcgcgctcc ggcgataaac gacactcggg ctggaaaaaa tctgcccgcg caaaagaaag
      301 ggaagcatat cgccgtcggc aaacgaagtc cagttggcgg tcgaatcgat aatttgctcg
      361 ttttcgtcaa ttaaaaacgc gtgtccgaaa gccagaaccg cgtcgggacg ccggcttaaa
      421 agcctgattc tttcttctaa aaaattcgga aaccagaggt cgtcagagcc gagataagca
      481 aaaaattcac tgccggtttt ggcaaaaccc tcgtttaaag tcgccgaaag cccgcgattt
      541 tcgcgcgcga taaattcatt ttcgaaaggg cattcgcgca aaacgcgccg gataatttcg
      601 gcggattcgt cttttgagcc gtcgtcaatt acaatcagtt tttttggcgg aaaagtctgc
      661 gcgaagattg aacgcaaagt tcgctcgacg aaaggcgcgt ggttgtaaga cggaacgaga
      721 acgctaacgg acattaaagc tattttagaa tccaaagtct ttatttgtca ttcgtccttt
      781 gtccattgtc cttcgtcgcc ggcgaaattt acattttgaa ttcccgcctg gatttgcaaa
      841 ggacgaatga cgaacggcaa aggacgaaca acgaaaatac tgcacacgct cgacacttta
      901 aaccgcggcg gcgcggaaat gcaggcgctg gacgtttgcc gcaacgcccg taaacacggt
      961 ttcgaaatga ctttcgtcgc tttcggcggc gggaaactgg aagaagattt tcgcgcctcg
     1021 ggcgttgatt tcattcaatc gcaacgccgc cttcccgtcg attttaacct gattttcaag
     1081 ctcagaaaaa tcatcaaaga ccgccgaatc gaaatcgcgc aggcttatca gcccgtcgaa
     1141 ggcttacatc tttatctcgc cgccatcggc ttaaacgtca aaaaggtttt gagctttcaa
     1201 ggctttatac aggacgcgaa aaaccggaaa accgcaaaat ttctaattcc gcgaatggac
     1261 gcgaacatcg tcgtcagcca aggcttgaaa aagtggctcg agcgcgcgga taatctggac
     1321 accggcaaaa attttcaagt cgtctataac ggcgccgacc gcgagagaat aatttcaaac
     1381 catccggttc tgcgcgccga gctgaattta agcgagaacg cgtcgctttt cggaatgatt
     1441 gcgaattttt atcgagaccc gcgcaaagac cagctaaccg tctgccgcgc gctgccgaaa
     1501 gttttcgccg aaatcgaaaa tgcccgatgc gtttttgtcg ggcgcaccga aacgggcgcg
     1561 gagcggaaat ttaaagaatg cgtcgatttt tgccgcgaga acaaaatcga cgaccgcgtt
     1621 cattttctcg gcgcgcgcaa tgacattccc gatgtgctgg cttcgctcga cgtttttgtt
     1681 ctttcgtcgc tgcacgaagg cttgcccgtc gccgccacgg aggcgatgct cgtcggcgtg
     1741 ccgatgattc tttccgatat cgagccgctt ctggaagttt ccgcaggcgg cagatacgcc
     1801 gaaatgtttc aaacgcaaaa cccggacgaa ct
//
LOCUS       DASWNE010000561         2494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_370794, whole genome shotgun sequence.
ACCESSION   DASWNE010000561 DASWNE010000000
VERSION     DASWNE010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370794"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..380)
                     /locus_tag="VGB68_11125"
     CDS             complement(87..380)
                     /locus_tag="VGB68_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049832.1"
                     /translation="MKTYYLKSIAVLLGCTLGGTIAAAIAGYLGGALGGLAMTFYNNN
                     LMTDTSPQGSYVWWANFCGWLIACFAFLPGGGTGFLFGLVKILEKPQLAVKNK"
     gene            complement(560..2080)
                     /locus_tag="VGB68_11130"
     CDS             complement(560..2080)
                     /locus_tag="VGB68_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049833.1"
                     /translation="MKYLGIILAIFSTSLAASAFQIEKRSPRMTNQQNKIFDEAEPRR
                     LLAAQPDYTASVSCLRYEKTFGHGYGESLKAAKKSGFYRLEAKTYIDYLSPDAPPVRY
                     SFKTKKFEVFSGDAENHLWFTKIESPALLVQDETLKFEIVGRESVDFQTNGKTKKREL
                     IKIKVTGETAVGGDWDKAEAFLYVAPDLKNLVVKTELFFPKAGRSCALADVSFIVPQE
                     LFKPFNEYRRRQAAKNSSFAASDAQSEKLEAVVISKAKSTLVSRIEPGLPRQPFAVWF
                     QSLVGQKTPVAWSVNDCGEQTGTAEDGGRDFPMCVEAAAEQSAGFFVFVNIQYGTFKR
                     GITAGKPVIRNIFSGDELQQGGASAESLKELRRTLMQRAIDAEIFDPNDGVFLIPREN
                     KPAGFEDFSEMYLQTKDYNRRNERVSVEPKGTVVIGQTDFAMREIVFDGKRWTFETVS
                     SGGTSYKFDGRFSKIKINVNGAQDGENVLRGRLIKLAGGKETAQADLIFSFVLQGE"
     gene            complement(2148..>2494)
                     /locus_tag="VGB68_11135"
     CDS             complement(2148..>2494)
                     /locus_tag="VGB68_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049834.1"
                     /translation="PVLTAGAPILSAAICGFLFGSTILDAEEIKSAGQAARRGLLVVF
                     LSYFLLFTVPFVISAFGSCLLNGFGGIIELLVFFAFIFFYGLFAVGWLAALVGAAAGF
                     LLYPLGLKSRTM"
BASE COUNT          614 a          667 c          572 g          641 t
ORIGIN      
        1 gattaatgct tgaagcgttc ggtcgcagct tcggattagc gagtattaaa gccaaatccg
       61 ccgaccttac tatttgtttt tcctgttcat ttgtttttaa ccgcaagctg cggcttctct
      121 aagattttta ccaacccgaa aagaaagccg gttcccccgc cgggtaaaaa cgcgaaacag
      181 gcaatcagcc agccgcaaaa attcgcccac caaacgtagc tgccctgagg cgatgtgtcg
      241 gtcataagat tattgttgta aaaagtcatc gccagcccgc cgagcgcgcc gcccaggtat
      301 ccggcgattg ccgcggcaat cgttccgccg agcgtgcagc cgagcaatac ggcgattgat
      361 ttaaggtaat aagttttcat tatttaagtg ttcatttttt cgacactcga ttgtttttca
      421 aggaatatct tcaagcgtat tcccgtcggc actgagctta cgcccgacga tttcgtcagt
      481 gataatcgct cgcgaggatt taattgcgat gaaccgtctg atgtcgaaat tatttttgtg
      541 cataattgtc cttttcattt tattcgccct gcaaaacgaa cgaaaaaatc aaatcagcct
      601 gcgccgtctc tttaccgccg gcaagtttaa tcaaacgccc gcgcaaaaca ttttcgccgt
      661 cctgagcgcc gttcacgtta atttttattt ttgaaaatct cccgtcgaat ttataactcg
      721 tgccgccgct tgaaacggtt tcaaaagtcc accgcttgcc gtcgaaaacg atttcgcgca
      781 tcgcaaaatc cgtttgcccg atgacgaccg tgcccttcgg ctcgaccgaa acgcgctcat
      841 tgcgccggtt ataatctttg gtctgaagat acatttcgga aaaatcttca aagcccgcag
      901 gtttgttttc gcgcggaatc agaaacacgc cgtcgttcgg gtcgaaaatt tcagcgtcta
      961 tcgctcgctg cattaatgtt cggcgcagct cttttaaact ctccgcgctc gccccgcctt
     1021 gttgaagctc gtcgccgctg aaaatatttc tgataacggg cttgcctgct gtaatgccgc
     1081 gcttaaaagt gccgtattga atgtttacga aaacgaaaaa accggcggac tgttcggctg
     1141 ccgcttcgac gcacatcgga aaatctctgc ctccgtcttc ggctgttccg gtctgctcgc
     1201 cgcaatcgtt gaccgaccac gcgaccggcg ttttttgccc gaccagcgat tggaaccaga
     1261 cggcgaacgg ctggcgcggc aaacccggct cgatgcgcga aaccaaagtg cttttggctt
     1321 tggaaatgac gacggcttcg agcttttccg attgcgcgtc ggatgcggca aacgatgaat
     1381 ttttagcagc ctgtcgccgc cgatattcgt taaatggctt gaaaagctcc tgcggcacga
     1441 taaacgaaac gtccgcgagc gcgcagcttc gaccggcttt cggaaaaaac aattccgttt
     1501 tgacgaccag attttttaag tcgggcgcga catacaaaaa agcttctgcc ttgtcccaat
     1561 cgccgccgac ggcggtttct ccggttactt tgatttttat caattcgcgt ttcttcgttt
     1621 ttccgttcgt ctggaaatca accgattctc tgccgacgat ttcaaatttc agcgtttcat
     1681 cctgaacaag caatgcgggg ctttcaattt ttgtaaacca cagatggttt tccgcgtcgc
     1741 ctgaaaaaac ttcaaacttt tttgttttga aagaatatcg aaccggcggc gcgtccgggc
     1801 tgagataatc aatgtaagtt ttcgcttcga ggcgataaaa accgctcttt ttcgccgctt
     1861 tcagcgattc gccgtaaccg tgtccgaacg tcttttcgta tctcaggcaa gacacgctcg
     1921 ccgtataatc cggctgcgcg gcgagcagtc gccgcggttc agcttcatca aaaattttgt
     1981 tttgttgatt tgtcattcgg ggcgagcgct tctcgatttg aaaagcgctc gcagcgagcg
     2041 aagtcgaaaa aatcgctaaa attatgccga gatatttcat ttgttttctt taaattgccg
     2101 gaagctcatt catctctccc gctgaaaatt tcgttttcca aaccggatta catcgtccgg
     2161 ctctttaacc cgagcggata taaaagaaat cctgccgccg ctccgactaa cgctgccagc
     2221 catccgacgg cgaacaaacc ataaaagaat atgaatgcaa aaaagacgag taattcgata
     2281 attccgccga agccgttgag caagcagctg ccgaacgcgc tgataacgaa cggaacggta
     2341 aataaaagaa aataagacaa aaaaacaacc aaaagcccgc gccgcgccgc ctgacccgcg
     2401 ctctttatct cttccgcgtc gagaatcgtc gaaccaaaca aaaagccgca gattgcggcg
     2461 gaaagaatgg gcgcgccagc cgtcagaacc ggag
//
LOCUS       DASWNE010000562         4359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_615473, whole genome shotgun sequence.
ACCESSION   DASWNE010000562 DASWNE010000000
VERSION     DASWNE010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615473"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2748
                     /locus_tag="VGB68_11140"
     CDS             <1..2748
                     /locus_tag="VGB68_11140"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0049835.1"
                     /translation="GDKLIGVSFDGGQRFEEVALPSDVQIFLEAAGIDGHLTYSLERT
                     SYSFSNRLYFADLVHIDTAPRWTAAIVFLTLVGLIWLGVGIFVLFKQGGHAPFVLHFA
                     VVCLAAFVFHVYKPLGLGADFDLAITLLDDLALAFFIPLFLHFCLRYPVRSEIFDTPR
                     WKTYALYAPAAIVGLADLFFSLTLALPLGEGFKLELAKFINAYSIFGIIGKTLEVHFV
                     IGIALGSIVLLWRFFKNRQPLVRQRLKWAVWGTIIAVLPVFAFQIAKRFVYLPDDALT
                     IGLTTVPLALIPLSFGHSVVRYRLMDVDVVVRRALVYAMTTLAIAAMIGTVALGLVYL
                     AIGNNLSTTEITLRALIAIVAMAGIVLLSEPLKNFLQERADRFFYGERYDMRRGLLDF
                     GRTLSSTTALDSLLNALVSRLQQVLDVEKVAVFIEDETNAKNYKLVRAVNLDLQTVPA
                     DFRQMIREKSAQKGIVRADELELETETSDPQNFVRQELHYYVPCVVRGRMVAVIGLGR
                     ASDGSLLSSEDLDILQTISGYVAVAIENSLLYQEQEKRADELALLKEFNESIVESVNV
                     GLLAVDENGRVTRSNSMFEEMLGLRREETVGKLVEELFDENFAHNLTQILGKSRWHLQ
                     ELRNAYKLHTTSTDGKPLILNVAVAPLRSVSNQQTGSIVVLENVTSRVKLEETLQQSE
                     KLSSIGLLAAGVAHEVNTPLTGVSSYTQMLLGMIPETDPKHALLLKVQKQTERATNIA
                     GNLLNFSRAGNGAGTEFGEIDVNKILDDTLQLLEPQLRKSRVDVIKKYLETPPKIYGN
                     AGKLQQIFTNLVLNARDAMFDGGTITLTTSIASDEVVVIEVADTGIGIEPENMSKIYD
                     PFFTTKGVGSGTGLGLAVTYGIVQEHAGTIEVQSEVGSGTTFRLEFPIAEKRPQQRAV
                     S"
     gene            2825..3658
                     /locus_tag="VGB68_11145"
     CDS             2825..3658
                     /locus_tag="VGB68_11145"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEY0049836.1"
                     /translation="MHEFVSFNQQMLAAGEAKISALGSAALYGSGVFTSLAIYNRKPF
                     QWEKHWQRLTENAEKLNIRLSSEFTEESVRNSLLELIEKNNLTGGRARITFFDEATSG
                     IWTFETVRRASLLIITGDFRNSAEEIRLTLSPHRINSKSPLAGVKSCNYLENLLALEE
                     AKSRQFDEAVRLNENGEMASAAMANLFWINDETIFTPSLGTGCLEGTTRAFVIEIAKE
                     LGFEIYTTAEHFDELRLADEVFITSAGLGIAGVGSVEGERCGDAVTSRLQREFAAKIP
                     R"
     gene            3794..>4359
                     /locus_tag="VGB68_11150"
     CDS             3794..>4359
                     /locus_tag="VGB68_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049837.1"
                     /translation="MDSKIGLAIQFTGIFLITVLSLFLRRSLKSAISGYWLVAWSSLS
                     ASLFCLSLAFSYEEFAKPLYTMYFFGEYIFGLMLVTGCHNLSGSESPTLRKKSIFLPF
                     IVIAFALTFLAQDFNLVFNLHAFILAAFFLAAFYVLRNSEIKTFGWRVMRVALLLLAL
                     DFFHYFILFSALLAGYDVSLPFGYLAFNP"
BASE COUNT          977 a         1130 c         1141 g         1111 t
ORIGIN      
        1 ggcgataaat taatcggcgt cagcttcgac ggcggacagc gttttgagga agtcgcttta
       61 ccgtccgacg tgcagatatt tctggaagcg gcgggcatcg acggtcactt gacttattct
      121 ctcgaacgca cttcctattc attttccaac cggctttatt ttgccgattt ggttcatatc
      181 gacaccgcgc cgcgctggac ggcggcgata gtttttctga cgctggtcgg gctgatttgg
      241 ctcggcgtcg gtattttcgt gcttttcaaa cagggcggac acgcgccgtt cgtactgcat
      301 tttgccgttg tttgtctcgc cgcttttgtt tttcacgttt acaaaccgct cggactcggc
      361 gcggatttcg atttggcaat caccttgctt gacgacttgg cgctcgcctt tttcatcccg
      421 ctttttctgc atttctgtct ccgttacccg gtgcgcagcg agattttcga cacgccgcgc
      481 tggaaaacct acgcgctcta tgcgccggca gcaatcgtcg ggcttgccga tttatttttc
      541 tctctgactt tagcgcttcc gctcggtgaa ggttttaaac tggaactggc gaaatttatc
      601 aatgcctaca gcatcttcgg aattatcggc aaaactctcg aagtgcattt cgtcatcgga
      661 atcgcgctcg gctcaatcgt tttgctctgg cgttttttca aaaatcgcca gccgctcgtt
      721 cgccagcgtc tgaaatgggc ggtttgggga acgattatcg cggttctgcc ggtgtttgcg
      781 tttcaaatcg ccaaaagatt tgtttatctg cccgacgacg ccttgacgat cggtttaacg
      841 acggttccgc ttgctttaat tcctttaagc tttggtcatt cggtcgtgcg ttatcgcctg
      901 atggacgtcg atgtcgtcgt gcgccgcgcg ctggtttacg cgatgacgac gctggcgatt
      961 gccgcgatga tcggaacggt cgcgctcggt ctggtttatc tggctatcgg caacaatctt
     1021 tcgacgacgg aaatcacgct gcgcgctctg attgcgattg tggcgatggc tggaatcgtg
     1081 cttttaagcg agccgctcaa gaattttttg caggaacgcg ccgaccgctt tttctacggc
     1141 gaacgctacg atatgcggcg cgggctactg gatttcggca ggacgctttc gtcgacgacc
     1201 gcgctcgact cgttgcttaa tgcgctggtc agccgtctgc agcaggtgct cgacgtcgag
     1261 aaggtcgccg tttttatcga agacgaaacg aacgccaaaa attacaaatt agtccgcgcc
     1321 gtcaatctgg atttgcaaac cgttccagcg gattttcggc agatgattcg cgaaaaatcg
     1381 gcgcagaaag gaattgtccg cgccgacgag ttggagctgg aaaccgaaac cagcgacccg
     1441 cagaattttg tccgtcagga gcttcattac tacgtgccct gtgtcgtgcg cgggcgaatg
     1501 gtcgccgtca tcggtttggg acgcgcctcg gacggttcct tgctttcatc ggaagatttg
     1561 gatattttgc agacgatttc cggctacgtc gccgtcgcga ttgaaaacag cctgctttat
     1621 caggagcagg aaaaacgcgc cgacgagctg gcgcttttga aggaatttaa cgaatcaatc
     1681 gtcgaatcgg tcaacgtcgg tctgctggcg gttgacgaaa acgggcgcgt tacccggtcg
     1741 aattcgatgt ttgaagaaat gctcgggctg cgccgcgagg aaacggtcgg gaaactcgtc
     1801 gaagagcttt tcgacgaaaa tttcgcgcac aatctgacgc agattctggg aaaatcgcgc
     1861 tggcatctgc aggagcttcg caacgcttat aagctgcaca cgacgagcac cgacggaaag
     1921 cccctgattt tgaatgtcgc ggttgcgccg ctgcgctcgg tttccaacca gcaaaccggc
     1981 tcgattgtcg ttctggaaaa cgtcacctcg cgcgtcaagc tcgaagaaac tctccagcaa
     2041 agcgaaaagc tttcgagcat cgggctgctc gccgccggtg tcgcgcacga agtcaacacg
     2101 cctctgaccg gcgtttccag ctacacgcag atgcttttgg gaatgattcc cgaaaccgac
     2161 ccgaaacacg cgcttttgct gaaggtgcag aaacagacgg aacgcgccac gaacattgcc
     2221 ggaaatcttt tgaatttttc gcgcgccggg aacggcgctg gaaccgagtt cggcgaaatc
     2281 gacgttaata aaattctgga cgatacgctc cagctgctcg aaccgcagct cagaaaatcg
     2341 cgcgtcgatg tcatcaaaaa atatctggag acgccgccga agatttacgg caacgcgggc
     2401 aagctccaac agattttcac caatctggtt ctgaatgcgc gcgacgcgat gtttgacggc
     2461 ggaacgatta ctttaacgac ctcgatagcg agcgacgaag tggtcgtcat cgaagtcgcc
     2521 gacacgggaa tcggcatcga gccggaaaat atgagcaaga tttacgaccc gtttttcacg
     2581 accaaaggcg tcggcagcgg aacgggttta ggcttggctg ttacctacgg catcgttcag
     2641 gaacacgccg gaacaatcga agtccagagc gaagtcggca gcggcacgac gtttcgcctc
     2701 gaatttccaa tcgcggaaaa acgcccgcaa cagcgcgccg tcagctaaaa agtgaaaaag
     2761 tgaaaaagtg aaagagtgaa aaagttttgt tgaataacaa gcgacctact ttttcacttt
     2821 cgatatgcac gaattcgttt cctttaatca gcagatgctc gccgccggag aagcgaaaat
     2881 ctcggcttta ggctccgccg cgctttacgg ctcgggagtt tttacctcgc ttgcgattta
     2941 caatcgaaaa ccttttcaat gggaaaaaca ctggcagcga ttgacggaga acgccgaaaa
     3001 attaaacatt cgcctttcct cggaatttac ggaagaatca gttcgaaatt cgcttttgga
     3061 gcttatagaa aaaaacaatc tgaccggcgg gcgcgcccga attacttttt ttgatgaggc
     3121 gacgagcgga atctggacgt ttgaaaccgt ccgccgcgct tcgcttttga taatcacggg
     3181 cgattttcgt aacagcgcgg aagaaattcg tctgacgctt tcgccgcatc gaatcaattc
     3241 aaaatcgccg ctcgcaggtg tgaaatcctg caattatctg gaaaatctgc tggctctgga
     3301 agaagccaaa agccgtcagt tcgatgaagc cgttcgcctg aacgaaaacg gcgaaatggc
     3361 ttcggcggcg atggctaacc tgttctggat aaacgacgaa acgattttca cgccttcgct
     3421 cggaaccggc tgtctggaag gaacgacgcg cgcgtttgtc atcgaaatcg caaaagagct
     3481 cggatttgaa atttacacga ccgcagagca ttttgacgaa ttgcgtcttg ccgacgaggt
     3541 ttttataacg tccgccggac tcggcatcgc cggggtcgga agcgtcgaag gcgagcgctg
     3601 cggcgatgcc gtgacgagtc gcctgcagag agagtttgcc gccaaaatcc cgcgataaga
     3661 aaaaaagtat ggacgcataa ggcgcgtttg taataaaatg taacatatgc aatcccacgt
     3721 gcatgttcca tccaatcaaa cagaagcccg aaagcctcta ttcaaacaat ctgccagcta
     3781 gttttccaac agtatggact caaaaatcgg cttagctatc caattcaccg gcattttcct
     3841 gataacagtt ttgtccctat tcctgcgccg ctcgctcaaa agcgcaattt ccggctactg
     3901 gctggtggcg tggtcgagcc tttccgcatc gttgttttgt ttaagcctgg cttttagcta
     3961 cgaggaattt gccaaacctt tatacacgat gtatttcttc ggcgaataca ttttcggctt
     4021 aatgctcgtc accggatgcc acaatctttc gggcagcgaa tcgccgacgc tccggaagaa
     4081 atcgatattt ttgcctttta tcgtcatcgc ttttgcgctt acatttctgg cgcaggattt
     4141 caatctggtg tttaatctgc acgcttttat cctcgccgcg ttttttctgg cggcgtttta
     4201 cgtgctcaga aattccgaaa tcaaaacttt cggctggcgc gtgatgcgcg tcgcgctgct
     4261 gcttctggct ttggatttct ttcattattt tattcttttt tcggcgctgc tagccggtta
     4321 cgacgtttct ttgcctttcg gttatctggc gtttaatcc
//
LOCUS       DASWNE010000563         2623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_54609, whole genome shotgun sequence.
ACCESSION   DASWNE010000563 DASWNE010000000
VERSION     DASWNE010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54609"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1179)
                     /locus_tag="VGB68_11155"
     CDS             complement(<1..1179)
                     /locus_tag="VGB68_11155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049838.1"
                     /translation="MKQSTRTINRFSFFNYARIIIGLSLIAAALHTASVYGQNSLNPA
                     GIDPTFTTSLSAGGYIEKTILQPDGKIVVIGVFNNANGTARRGAVRLNSDNSLDASFN
                     MPNTVNPRSMELLPDGKFIAFGFPSIVRLNSDGSIDPSFNFARTILGGSVPTLKPRSD
                     GKVIVYGSFKVTKGRLVVAENFILVDQFGGIESGFRPDITGHMIDVVLMPNGKTVIAG
                     NFVWRHLGQIIGKNLAVLNEDGTPDLNYSGFYGKQGEYTRGATLQSDGKLLVYGTFTE
                     AYKAPPAGKSVKNLGILRLNADGSFDRNFSVPFVSYSEVSSAFVQPDGKIVLRGSFSL
                     ANGSSYYFLARIFSDGGFDQSYANNLPFAPNLFQSPSKMEMQADGRILMAGDLKVINN
                     P"
     gene            complement(1528..>2623)
                     /locus_tag="VGB68_11160"
     CDS             complement(1528..>2623)
                     /locus_tag="VGB68_11160"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0049839.1"
                     /translation="WSAGEAERFTGEVVPVDTIATGKGRTAFTERVPRGVIYGITPFN
                     FPLNLVGHKVAPALASRNAIIVKPSSKTPLTALLLGEVFLESGLPKAALQVVPTDVKY
                     IDAIFKDERVAMITFTGSAAVGWRLKTRAERKMITLELGGNAPVIIDESADVDKSLDR
                     NLMGAFTYAGQTCISVQRVYIHEKIFSEWTEKFVERAKKIKVGNPLDDATELSAMIDE
                     EAAKKAELILNEARQGGAEILCGGGRAGAMLDAAVITGTNPEMRAVSEEIFAPVAVVE
                     KFSNFAEAVEMSNQTKYGLQAGVFTQDLANSQYAAQNLEYGGVMINDVPTFRVDNMPY
                     GGVKQSGFGREGTRYAMEEMSEIKLVVINQ"
BASE COUNT          618 a          664 c          646 g          695 t
ORIGIN      
        1 cgggttatta atgactttca ggtctcccgc cattaaaatt ctgccgtccg cctgcatctc
       61 cattttagac ggcgattgaa aaagattggg cgcaaacggc agattgttag cgtaagactg
      121 gtcgaagccg ccgtcggaaa aaattctggc aagaaaataa taactggagc cgttagccaa
      181 agaaaagctt ccccgcaaaa ctattttgcc gtccggctga acgaatgccg aggacacttc
      241 cgaatacgaa acgaacggga cggaaaaatt gcggtcaaaa gagccgtcag cgtttaaacg
      301 aagtattccc aggtttttta cagacttacc tgccggaggc gctttataag cctcggtgaa
      361 agtgccgtaa actaataatt ttccgtcgga ctggagcgtc gcgccgcgag tatattcacc
      421 ttgtttgccg taaaatccgc tgtaatttaa atcgggcgtg ccgtcttcat tcaaaaccgc
      481 cagatttttg ccgattatct gtcccagatg cctccacacg aaattgccgg cgataaccgt
      541 ttttccgttt ggcattagaa cgacgtctat catatgcccg gtaatgtccg gtcgaaaacc
      601 cgattcgata ccgccaaact ggtcaaccaa tataaaattt tcggcaacaa ccagtcttcc
      661 ctttgtaact ttgaaacttc cgtagacaat cacttttccg tcacttcgcg gcttaagcgt
      721 cggcacgctg ccgcccaata tagtgcgggc aaagttaaag ctcgggtcaa tcgagccgtc
      781 ggaatttaag cgaacaatcg acggaaagcc gaaggcgata aatttcccgt ccgggagcaa
      841 ttccatcgag cgcgggttta cggtgttcgg catattaaag gaggcgtcga gcgaattatc
      901 cgagttcagc cggacggcgc ctcttctggc agtcccgttt gcgttattaa agacgccgat
      961 aacgacaatc tttccgtcag gctgcaaaat cgttttttcg atatatccgc ccgcgctgag
     1021 gctggtcgta aaagtcgggt cgattccggc aggatttaaa ctgttttgcc cgtaaacgct
     1081 tgctgtatga agcgccgctg cgatcagcga aagaccgatg attatgcggg cgtaattaaa
     1141 aaaggaaaag cgattgatgg ttcgggtact ttgtttcatt tgatgtctct tttagtcgcg
     1201 caggttttat tttttgaaga tttacggatt cagccgcact tatccggctg gtctcgcgcc
     1261 gttgattatc ttaaaggttc cggcatacag aatttttccg tccggctgaa ggctgattgt
     1321 tactcgtata atttttaaaa aggaaaaccg ggtttgcatt tcagactgtc gttacatttg
     1381 attgctccga aaaattcgat tggagcagcc tggaaaggaa aataagatta aaaccggaaa
     1441 ggaaccggat tatataataa atgaaaatgt taaacaaaag caatattttg tttggcattt
     1501 cttaacgtat tgctttttgc ttatttgtta ttgattaatg acgaccagct taatttcgct
     1561 catttcttcc atcgcgtagc gcgtgccttc gcgcccgaag ccggattgct tgacgccgcc
     1621 gtacggcata ttgtccacgc gaaaggtcgg cacgtcgttt atcatcacgc cgccgtattc
     1681 gagattttgc gcggcgtatt gcgaatttgc gaggtcctgc gtaaaaactc cggcttgcaa
     1741 gccgtatttc gtttgattgg acatttccac cgcttccgca aaattcgaaa atttttcgac
     1801 gacggcgacc ggcgcgaaaa tttcttccga gacggcgcgc atttccggat tcgtgccggt
     1861 aatgacggcg gcgtcgagca tcgcgcccgc gcgcccgccg ccgcataaaa tttccgcgcc
     1921 gccctggcgg gcttcgttca ggattaattc ggcttttttc gccgcttcct cgtcaatcat
     1981 tgccgataat tcggtcgcgt cgtccagcgg atttccgact tttatttttt tggcgcgctc
     2041 gacaaatttt tccgtccatt cggaaaagat tttttcgtgt atgtaaacgc gctgaaccga
     2101 gatgcaggtt tgcccggcgt atgtgaaagc gcccatcagg tttctgtcca gagatttatc
     2161 gacgtcagcc gattcgtcga tgatgacggg cgcgttgccg ccgagttcga gcgtaatcat
     2221 tttgcgttcg gcacgcgtct ttaatcgcca gccgaccgcc gcgctgcccg taaaagttat
     2281 catcgcgacg cgttcgtctt taaaaatcgc gtcgatgtat tttacgtccg tcggcacgac
     2341 ttgcagcgcc gctttcggca agccgctttc gagaaaaacc tcgccgagca gcaaggcggt
     2401 cagaggcgtt ttcgaactcg gcttgacgat gattgcattc cttgaagcaa gcgcgggcgc
     2461 gactttgtgt ccgaccagat tcagaggaaa attaaacggc gtgatgccgt aaatgacgcc
     2521 gcgcggaacg cgttcggtaa acgccgttct gccttttccc gtcgcaatcg tatcaaccgg
     2581 cacgacctcg cccgtaaatc tttcggcttc gcccgccgac caa
//
LOCUS       DASWNE010000564         3065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_331035, whole genome shotgun sequence.
ACCESSION   DASWNE010000564 DASWNE010000000
VERSION     DASWNE010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331035"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VGB68_11165"
     CDS             complement(<1..551)
                     /locus_tag="VGB68_11165"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049840.1"
                     /translation="MSRIFSQVLCLAIIAFFMGQAKAQTGSFDVIDYDARIEPDMTSK
                     SVKGKVSIRFSSLKNNLSEIHLSAGVLEIDAAREGKTALKFEKRERVLIISLPRPAKF
                     GEKREIEIEYHGAPRFGIRFFPDERQVYTLFSTSQWMPCVDSPDDRATFRLNLILPKD
                     LKAAGNGRFIRERALPDNKISSE"
     gene            complement(588..758)
                     /locus_tag="VGB68_11170"
     CDS             complement(588..758)
                     /locus_tag="VGB68_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049841.1"
                     /translation="MLLTKIVVFDSLALDDYDSAITYPAVLRDLGEPAAGNHVEFLYV
                     ADEKPRFQYRLF"
     gene            complement(912..1334)
                     /locus_tag="VGB68_11175"
     CDS             complement(912..1334)
                     /locus_tag="VGB68_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049842.1"
                     /translation="MKTNKLQSKIRLFGLAFALLFGLGIMSVTSVSAQDRDYRRDDRR
                     DNRNDDRYDNNRSNSYRTAMQQGYREGLQSGREDARNNRRENAMRAYNRAVREARRNY
                     NSRYGNQNAFSQAFREGFMRGYNEGYDRVDDRRDRRRN"
     gene            complement(1657..2457)
                     /locus_tag="VGB68_11180"
     CDS             complement(1657..2457)
                     /locus_tag="VGB68_11180"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0049843.1"
                     /translation="MNKNNARHNHKKYSRFRFVSEPLDAIDRNDEIRKPDFPFSKELE
                     KTRYPVRALRYWWMHCVIQDELKRLKRAPVIVDVGCDRGIIKRFIPPVEGARWIGLDI
                     DTNREGIGLAKYDELHQCDFDEGLPLADASVDIAICSHVLEHLPRPDFTMREIARILR
                     PGGLLLVGVPTAPKFIAHFRERQFARQLQAGTRQRGQHVHVFWSKRLRRIAEQVGLQV
                     EFSTGTALIRKKGSRLENYAWWIRFNQVWAALFPSLGQELCLQLRKPV"
     gene            complement(2569..>3065)
                     /locus_tag="VGB68_11185"
     CDS             complement(2569..>3065)
                     /locus_tag="VGB68_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049844.1"
                     /translation="NRNKKKKLLQRIDKKYIGFHVYRSEIENLPFAFWTRLTDEPIPE
                     GDFNDAAAAVGKRYFYKLTRVDAEGNESAPITPKSTYTDHAGNRFGQNPMVDFAGYNI
                     YRSADESVALERWERRNTEPLPTTEFRDEGVASGEIYFYYVRAVDSKGTESAPSEVVR
                     VIRK"
BASE COUNT          743 a          794 c          741 g          787 t
ORIGIN      
        1 cattcggacg agattttgtt gtcgggcagc gcgcgttcac gaataaaacg accgttgccc
       61 gccgctttta aatccttcgg caaaatcaga ttcaggcgaa acgtggctct gtcgtccggc
      121 gaatcgacgc acggcatcca ctggctcgtg gaaaaaagcg tataaacctg ccgctcgtcg
      181 ggaaaaaatc tgatgccgaa acgcggcgcg ccgtgatatt caatctcaat ttcgcgcttt
      241 tcgccaaatt tcgccggacg cggcagagaa ataatcaaaa ctctttcgcg cttttcaaat
      301 ttcaaagcgg tttttccttc gcgcgcggcg tcgatttcca gaacgcccgc actcagatga
      361 atttccgaga gattgttttt cagcgagctg aagcgaatcg aaactttgcc tttgacggat
      421 ttgctcgtca tatccggctc aatccgcgca tcgtaatcga taacatcgaa agagcctgtc
      481 tgcgctttcg cctgtcccat aaaaaacgcg ataatcgcta aacacaaaac ctgagaaaaa
      541 attctgctca tttttccatt aacgaccaaa ccgctgctaa aatcaaccta aaaaagacga
      601 tactgaaaac gcggtttttc gtctgcgacg tatagaaact cgacgtgatt tcccgcggcg
      661 ggctcgccta aatcgcgcag aacagccggg taagttatgg ctgagtcgta atcgtccagc
      721 gcgagcgaat caaagacgac gatttttgtc agcagcataa agcaggaatt tttcttttcg
      781 ttcggcggaa tttcgagggc ggctgaatcg agtttatttt caggttatat cgaggcgaaa
      841 aaaggcgcag tctcgaatga aactgcgcct tcgtcctgtt atttattaaa caagagattt
      901 attttttagt atcagttacg gcgacggtcg cggcggtcgt ctacacggtc gtaaccttcg
      961 ttatagccgc gcataaaacc ttcacggaaa gcctgcgaaa acgcattctg gttgccgtag
     1021 cgcgagttgt agtttctgcg agcttcacgc acggcgcggt tataagctct cattgcattc
     1081 tcgcggcggt tattgcgggc gtcttctcgt ccgctctgta aaccttcacg ataaccttgc
     1141 tgcatcgccg tgcgatagct gttgctgcgg ttattgtcgt aacggtcgtc attacggtta
     1201 tcccggcggt catcccggcg gtaatcacgg tcttgcgcgc tgacgcttgt caccgacata
     1261 attcccaaac cgaaaagtaa tgcgaatgcc aagccgaata atctgatttt gctttgtaat
     1321 ttgtttgttt tcataagaac tccttgagaa cttcctttct cgcgtttact aaagcgatta
     1381 tcgtgccacg caaaaaatat caataaatta tagatttttt acaaaatccg cgaccgccgc
     1441 ttctatcttt tgccgcaaat cggttatcga cagtctattt gagtatgttg cggcgattcg
     1501 atgcacagag atttccggga aaatgaaatc acagataaac gccgataaat taaaacgcgg
     1561 atagaagagg ataattcaaa acccggaaca ttgaatttta tcttgaatat cctgtccata
     1621 ctgtttgagt tttctctgta tctttgcggc ttatattcag acaggctttc gcagctgcag
     1681 acataattcc tgaccgagcg agggaaacag cgccgcccag acctgattga aacgaatcca
     1741 ccaggcataa ttttccagcc ggctgccttt ttttcggata agcgccgttc cggtcgaaaa
     1801 ttcgacctgc aagccgacct gttcggcgat gcggcgcagg cgcttcgacc aaaaaacgtg
     1861 gacgtgctga ccgcgctggc gcgttccggc ttgcagttgt ctggcaaact gccgctcgcg
     1921 aaaatgagcg ataaatttcg gcgcggtcgg cacgccgact aataaaagac cgcccggacg
     1981 aagaatccgc gcaatctcgc gcatcgtaaa atcggggcgc ggcagatgct ccagcacgtg
     2041 cgagcagatg gcgatatcga ctgaggcgtc cgccagcggc agcccttcgt caaaatcgca
     2101 ttgatgcagc tcgtcgtatt ttgccagccc gatgccttcg cggttcgtgt cgatatccag
     2161 cccgatccag cgcgcgcctt cgaccggcgg aataaaccgc ttgataatcc cgcggtcgca
     2221 cccgacatcg acaatcacgg gcgcgcgctt caatcgcttt aattcgtcct ggatgacgca
     2281 gtgcatccac cagtagcgca aagccctgac cggataccgc gttttttcca gctcttttga
     2341 aaaagggaag tccggcttgc gaatctcgtc attgcggtca atcgcgtcca gcggttcgga
     2401 gacgaatcgg aaacggctgt attttttatg attatggcgc gcgttattct tgttcatcgg
     2461 taattccttt aaacgggtga agaaatagaa taaccaaaat agatgaaaag tgtgagctga
     2521 taactgaaag ttgaaaacag ttatcagttc tcagctatca gttctcagtt atttcctgat
     2581 aacgcgcacg acttcggacg gcgcgctttc cgttcctttc gaatcgacgg cgcgcacata
     2641 ataaaaataa atctcgcccg atgcgacgcc ttcgtctcta aactcggtcg tcggcagagg
     2701 ctccgtattg cgccgttccc agcgttcgag cgccacgctc tcgtcggcgg agcgataaat
     2761 attgtaaccg gcgaaatcga ccatgggatt ttgcccgaag cggtttccgg cgtggtccgt
     2821 ataagtgctt ttcggcgtaa tcggcgcgct ttcattgcct tcggcgtcaa cccgcgtcag
     2881 cttgtaaaaa taacgctttc cgacggcggc agccgcgtcg ttgaaatcgc cttccggaat
     2941 cggctcgtcg gtcagacgcg tccagaatgc aaacggcaga ttttcaattt ccgaacgata
     3001 aacgtgaaag ccgatgtatt ttttgtcgat tctctgcaaa agcttttttt tcttattcct
     3061 gtttt
//
LOCUS       DASWNE010000565         1575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_123973, whole genome shotgun sequence.
ACCESSION   DASWNE010000565 DASWNE010000000
VERSION     DASWNE010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123973"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(730..>1575)
                     /locus_tag="VGB68_11190"
     CDS             complement(730..>1575)
                     /locus_tag="VGB68_11190"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0049845.1"
                     /translation="TAVINLWGSTQGEMAVVPSFGLLVNFPRLTTFAGFGQPQLEFSQ
                     AVVQRDRRLDDLRKTFKDAENYARVKDAYAKDRSLPYPGVNLKMDAMIPFIRGERPVI
                     FRAERERDIRAIVRFVDEMKIKGVIIGGQEAWKAADLLAKNNIPVIYTNIYNLPVRDD
                     DEYDNLFEAPSKLQKAGVKFAISTGNDGAEVRDLPYHAGLASAYGLSKEEALKSVTLY
                     PAQILGISDKFGSIEQGKTANIVVADGDLLEPRTNVRYLFINGRMLPLTSRHTELYER
                     FKDRK"
BASE COUNT          394 a          394 c          376 g          411 t
ORIGIN      
        1 gaaaatgcgg tagactttca ccgtattttc atccggaaaa attgctgatt gtaaattcaa
       61 agataccaaa cggtaattta ccgacattag acagccttaa tactctaatc gcgcagggtt
      121 aagcgggcga ctctctttaa acaataaaca gacgtcgccc gtcccggcac tggaaaaaca
      181 aaaaccaatt aaactatcaa tccccagccg cgactcgtcg cgctttgaca gttttacacg
      241 gtaattttgt ttctgatact cttagattta tttatagaca tcgtaaaatg ttctgaaaaa
      301 ttcccctaag tagaaatttt catcccagga tgcccggttg gtcggttaat ctaaagcgaa
      361 cctaacacac taaataatcc ctgtcaaaga acgcgtgttt acgttcattc cggcgtttgc
      421 gctaaacggc tgcccgatag gacttcgcgg tgcgaaagcg aattaaatat tttatttgcg
      481 gctcgtcgat taagatttaa tcccgaccgc aaaatccgct ttcagcgcaa aatcctgaaa
      541 acgagtgttt gtgaaaatat ctttgattgt ttaattcggc ggcagaggta aaggaaattt
      601 atttcaccgg cgagtcttcg ggcgcggctc ttatgcttcg agcgcggaac gagatggcgg
      661 aaaagaaaaa ggcgtctcga cggacgcctt ttcctatcat ttaaatcatt tcatttaaat
      721 catttaaaat tattttctgt ctttaaaccg ctcgtaaagc tccgtgtggc ggctcgtcag
      781 aggcagcatc cgaccgttga taaacaaata tctgacattc gtgcgcggtt cgagcaaatc
      841 gccgtcggcg acgacgatgt tcgccgtttt tccctgttca atcgagccga atttatctga
      901 aattccgaga atctgcgccg gatagagcgt cacggatttt aaggcttctt ctttcgataa
      961 accgtaggcg gacgccagcc ccgcgtgata aggcaaatcg cggacttccg cgccgtcgtt
     1021 gccggtcgag atggcaaatt tcacgcccgc tttctgcaat tttgacggcg cttcaaacaa
     1081 attatcgtat tcgtcgtcgt cgcgcacggg caggttgtaa atgttcgtgt agataacggg
     1141 aatattgttt ttagccagta aatccgccgc tttccacgct tcctgcccgc cgatgatgac
     1201 gcctttgatt ttcatttcgt cgacgaaccg gacgatggcg cgaatgtccc gctcgcgttc
     1261 ggcgcggaag attacgggac gctcgccgcg aataaaggga atcatcgcgt ccattttcag
     1321 attgacgccc ggatacggca agctccggtc tttcgcgtag gcgtctttta cgcgcgcgta
     1381 attttcggcg tctttaaaag ttttccttaa atcgtccagc cgccggtcgc gctggacgac
     1441 ggcttggctg aattcgagct gcggctgacc gaaaccggca aaagtcgtca ggcgcgggaa
     1501 attgacgagc aagccgaaag acggcacgac cgccatttcg ccctgcgtcg agccccagag
     1561 attgatgacc gcggt
//
LOCUS       DASWNE010000566         2414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_615552, whole genome shotgun sequence.
ACCESSION   DASWNE010000566 DASWNE010000000
VERSION     DASWNE010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615552"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="VGB68_11195"
     CDS             <1..945
                     /locus_tag="VGB68_11195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEY0049846.1"
                     /translation="GMSRFRANLFNQKGAVGCVFRAIPYEIKSFDALGLPPVVSDMCK
                     KPRGLVLVTGPTGSGKSTTLASMIDKINIDRRDHILTIEDPIEFLHNHKNCVVNQREV
                     GADTHGFADALRTALRQDPDIVLVGEMRDLITIEMALRIAETGHLTFATLHTNSAAST
                     INRIIDVFPAEQQSQVRTQLSLVLEGILCQSLLPKASGDGRVMALEILIPNSAIRNLI
                     REDKIHQIYSMMQTGQDKFGMQTFNQALATLFQKRLITLDVAMQRSSNADELKDLIER
                     GSGLNQAYGGQAKPGASSQNHASPYMQGRPVGQRPPVR"
     gene            1057..2289
                     /locus_tag="VGB68_11200"
     CDS             1057..2289
                     /locus_tag="VGB68_11200"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY0049847.1"
                     /translation="MPTFVYKGRNRLNEMVSGEREAASQDELRALLRREQVVLTQASE
                     KGREFSIPKLGAKKKVKAKELSVFTRQFSVMIDAGLPLVQCIDILAQQQQNPFFKDVL
                     NQVRQHVEEGSTLHSALEKHPKVFDSLYTSMVEAGETGGVLDLILQRLATLIEKVVKL
                     KRSIVSASIYPAAVILVAIAAVAIIMVVVIPQFEQIFLGLLGPGEALPLPTRIVMAIS
                     NFLAGWGGIATLVFLIGAAVGTSYYYKTPRGRHQIDALLLKLPIFGSILRKIAVARFS
                     RILSTLLSSGVPILQSLEITAKTAGNVIVEDAIMKVRAGVERGENFVEPLKATEVFPH
                     MVGQMVGVGEQTGALDAMLGKIADFYEDEVDSAIADLLALIEPALIAFLGVTIGSIVI
                     SMYLPLFTLIGKLASGAK"
BASE COUNT          596 a          657 c          603 g          558 t
ORIGIN      
        1 ggaatgtcgc gttttcgcgc caatcttttc aaccagaaag gcgcggtcgg gtgcgttttc
       61 cgcgcgattc cctacgaaat taaatcattc gacgctttag gcttaccgcc cgtcgtttcg
      121 gatatgtgta aaaaaccgcg cgggctggtg ctggtcaccg gtccgaccgg ctccggtaaa
      181 tcgacgacgc tggcttcgat gattgacaaa atcaacatcg accgccgcga ccacattctg
      241 accatcgaag acccgattga atttttgcac aatcacaaaa actgcgtcgt caatcaacgc
      301 gaagtcggcg cggatacgca cggttttgcg gacgccttac gcaccgcgct gcgccaggac
      361 ccggacatcg ttctggtcgg cgaaatgcgc gacctcatca ccatcgagat ggctctgcga
      421 attgccgaaa ccggtcactt aacttttgcc actctgcaca caaactcagc cgcctcgacc
      481 attaaccgca tcatcgacgt ttttccggcg gaacagcaat ctcaggtgcg cacgcagctt
      541 tcgcttgttc tggaaggaat tctttgccaa tcgctgctgc cgaaagcctc gggcgacggg
      601 cgcgtgatgg ctctggaaat tctcattccg aattcggcaa ttcgcaatct gattcgcgaa
      661 gacaaaattc accagattta ttcgatgatg cagaccggac aggataaatt cggtatgcag
      721 actttcaatc aggcgctcgc cacgctcttt cagaaacgcc tgattacgct ggacgtagcg
      781 atgcagcgtt cttcaaacgc ggacgaactg aaagatttaa ttgaacgcgg ctcgggtctt
      841 aatcaagctt acggcgggca ggcaaaaccg ggcgcctcgt cgcaaaatca cgccagcccg
      901 tatatgcagg gtcgccccgt cggacagcgc ccgccggttc gttagaaact caggattcga
      961 gggttcagga gcgagccgag atttaaatcc tgaattccaa atcataaatc ctgaattcta
     1021 aattctgaat taaaatcaca ggagctagag acagaaatgc caacatttgt ttataaaggt
     1081 cgaaatcgct taaatgaaat ggtttcaggc gagcgcgaag ccgccagcca ggatgagctg
     1141 cgtgcgcttc taaggcgcga gcaggtcgtt ctgacgcagg catcggaaaa gggacgcgag
     1201 ttcagcatcc cgaagctcgg cgcgaagaaa aaggtcaaag ccaaagagct ttcggtcttc
     1261 acgcgccagt tttccgtgat gattgacgcc ggtttgccgc tcgtccaatg tatagatatt
     1321 ctggcgcagc agcagcagaa tccgtttttc aaagacgttt taaatcaggt tcggcagcac
     1381 gtcgaagaag gttcgacgct tcactcggcg ctggaaaaac acccgaaagt tttcgacagt
     1441 ctttacactt cgatggtcga agccggcgaa acgggcggcg ttctggattt gattcttcag
     1501 cgtctggcga ccttgattga aaaagtcgtt aaattaaaac gcagcatcgt ctcggcttcg
     1561 atttacccgg cggcggtcat ccttgtcgca atcgcggcgg tggcaatcat tatggtcgtc
     1621 gtcatcccgc agtttgaaca aatcttcctc gggcttctcg gtccgggcga agctctgccg
     1681 ttgccgacgc gtatcgttat ggcaatcagt aatttcctcg ccggctgggg cggaatcgcc
     1741 acactggttt tcctgattgg cgcagccgtc ggcaccagct attattacaa aacgccgaga
     1801 ggtcgccatc aaatcgacgc gcttttgcta aaactcccaa ttttcggaag cattctccgc
     1861 aaaatcgccg tcgccagatt ctcgcgcatc ctttcgacgc ttctgtcctc gggcgtcccg
     1921 attctgcaat ctctcgaaat tactgcaaaa accgccggta acgtcatcgt cgaagacgcg
     1981 attatgaaag tccgcgccgg cgtcgaacgc ggcgaaaact tcgtcgagcc tttaaaagcg
     2041 acagaagtct tcccgcacat ggtcgggcag atggtcggcg tcggcgaaca aaccggcgca
     2101 ctggacgcca tgctcggaaa aatcgccgac ttttacgaag acgaagtcga ttcagcaatc
     2161 gccgacctct tagccttgat tgaacccgcc ctaatcgcct tcctgggcgt aactatcggc
     2221 tcaatcgtca tctcaatgta tctaccgctg tttactttaa tcggaaaact cgcaagcggt
     2281 gcgaaatagc ctgataggcc tgagaggtaa ccataaacta ccaaaagtgg gaatcataat
     2341 ctaccatttg aggtattcat attcttccac ttttatctaa actgaacaaa atataaagtt
     2401 acaataaggt ggtt
//
LOCUS       DASWNE010000567         1861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_302246, whole genome shotgun sequence.
ACCESSION   DASWNE010000567 DASWNE010000000
VERSION     DASWNE010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302246"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..>1861)
                     /locus_tag="VGB68_11205"
     CDS             complement(78..>1861)
                     /locus_tag="VGB68_11205"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY0049848.1"
                     /translation="ILEAIEFERSQAKPDKQLILLGLTAILRTRPAKGDFIASKFLTS
                     PDAQIRADAANTLARLRAKSYNEALRAMLLADDDAAARANAARVLGIAEDKTALTLLI
                     KSAAEDGDSRVRVSAIRSLGSLKDKKAVAPLLDYGNKLLDAYTSAKAKNANPPQKNEL
                     LEIATTLGRLLPNSNDESALEFLRNFRALDKFQSPETEIAFARVAPEKYLTEFKTKNN
                     GYADWRIASAYAQGLSDLATLENVSLKLRAAETLTSYAGGMVTGVKTAYQGEMTKALP
                     DLIRALAAFKPDNLDEILRSMFENPDIFVRAATAEIIGERPLTEENFKALERAFVKAL
                     LADNDYNDAQLAIMDALFKLDRQKSTGNLLVALNSKDYIVRKKAFELLRTKDLEKDSP
                     GIPTMLEVASGKGQERVLPYGYGSRLGQILNAQADYTRAVSRKNGQVKAVVTTEKGAF
                     TIDFFPEDAPLAVDNFVKLARAGYYNNVSIHRVVPNFVMQDGDPRGDGNGGPGWQIRC
                     EINRIPYERGAVGMALSGKDTGGSQWFVTHSQQPHLDGGYTVFGRVNESDMKIVDSLA
                     RGDKILSVKIVEGNLPQRTQKTVRKRN"
BASE COUNT          387 a          530 c          483 g          461 t
ORIGIN      
        1 atttacacgg gtgaacaacg ataagaaaca gataagaaaa gatattactc cgtcaatccg
       61 gtttcagtcc gctgcggtta atttctcttt ctcaccgttt tctgcgtcct ttgcggcaaa
      121 tttccttcaa cgattttaac gctcagaatt ttatcgccgc gcgcgagact atctacgatt
      181 ttcatatcgc tttcgttcac gcgcccgaaa accgtgtagc cgccgtccag atgcggctgc
      241 tgcgaatgcg tcacgaacca ttgcgagccg cccgtgtctt tgcccgacag agccattccg
      301 accgcgccgc gctcgtaagg aatccggttg atttcgcagc gaatctgcca gcccggaccg
      361 ccgttgccgt cgccgcgcgg gtcgccgtcc tgcatcacga aattcgggac gacgcgatga
      421 atcgaaacgt tgttgtaata accggcacgc gccagcttga cgaaattatc gaccgccagc
      481 ggcgcgtctt ccggaaaaaa atcaatcgtg aacgcgcctt tttccgtcgt cacgacggct
      541 ttcacctgcc cgtttttacg cgagacggcg cgcgtgtaat cggcttgcgc gttcagaatc
      601 tgcccgagcc gcgagccgta gccataaggc aaaactcttt cctgtccctt tccgctggcg
      661 acttccagca ttgtcggaat accgggcgaa tctttttcca aatctttcgt ccgcaacagc
      721 tcgaaggctt ttttgcggac gatataatct ttggaattga gcgcgaccag aagattgccg
      781 gtcgatttct gccggtctaa tttgaacagc gcgtccataa tcgccagctg cgcgtcgttg
      841 taatcattat cggctaagag agctttgaca aaagctctct ccagagcctt gaaattttcc
      901 tcggtcagcg gtcgctcgcc gataatctcg gcggtcgccg cgcgcacgaa aatatcggga
      961 ttctcaaaca tactgcgcaa aatttcgtcg agattatcgg gtttgaaagc cgccagagcg
     1021 cgtatcaaat ccggcagagc tttcgtcatt tcgccctgat aagcagtctt tacgcccgtt
     1081 accattccgc cggcataaga cgttaaagtt tcggcggcgc gcagcttcag cgaaacgttt
     1141 tccaaagtcg ccaaatcgct caagccctgc gcgtaagccg aagcgatgcg ccaatccgcg
     1201 taaccgttat ttttcgtttt gaattcggtc aggtattttt cgggcgcgac gcgggcgaaa
     1261 gcgatttccg tctccggcga ttgaaattta tcgagcgcgc ggaaattccg caaaaattcc
     1321 agcgcgcttt cgtcgtttga attcggcaaa agcctgccga gcgtcgtcgc aatctcaagc
     1381 aattcgtttt tctgcggcgg gttcgcattt ttcgcttttg ccgatgtata agcgtcgaga
     1441 agcttgtttc cgtaatccag caaaggcgcg acggcttttt tgtcttttaa gctgccgaga
     1501 gagcgaatcg cgctgacgcg gacgcgcgaa tctccgtcct ctgccgccga tttaatcaaa
     1561 agcgtcaacg cggttttgtc ttcggcgatt cctaaaacgc gcgccgcgtt tgcccgcgcc
     1621 gccgcgtcgt cgtccgcgag aagcatcgcg cggagcgctt cattgtagct tttggcgcgc
     1681 agccgagcca gagtgttggc ggcgtcggcg cgaatctgcg cgtccggact ggtaagaaat
     1741 ttcgaagcga tgaaatcgcc tttcgccgga cgcgttcgca aaatggcggt tagaccgagc
     1801 agaatcaatt gtttgtccgg tttcgcctgg ctccgttcga actcgatggc ttcgagaatc
     1861 g
//
LOCUS       DASWNE010000568         2503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_302261, whole genome shotgun sequence.
ACCESSION   DASWNE010000568 DASWNE010000000
VERSION     DASWNE010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302261"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..1372)
                     /locus_tag="VGB68_11210"
     CDS             complement(134..1372)
                     /locus_tag="VGB68_11210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0049849.1"
                     /translation="MKLSENGNFNHLPKKVLCVWNYLEWGGAQVYFLALMKEAKKHCE
                     VRAVLPEGSNEQLLKFLKDAGIPCEFVDSHTDSQPALTVKRKLARHFNKLRSEYVFCR
                     YLKKVGLKDCAVHVEFAPWQSFAALFWLSTKTKVFTTVHNSVRPQQWWRDWIWKFKFG
                     VLSKFKNFHIFTANEDAKKCLREFVSADFYERVKVTYASINPPELLNAQKAELDERAI
                     RQKFNIPADKFLVLCVGQFIDRKGRWIFLEAAQKLLKKKSDAAFVWISNSKPNDEDLR
                     KAESYGLGENFVFITSEQVGKERGDLFNLMRLADVFALPSFVEGLPISMLEAMALGKA
                     VVSTNINGIPEAVKHMETGWLIEPNDVDALVEAIETLKNDEALREKLGRTGSEFVLKN
                     FDERIAAQTAWQSYEESFSE"
     gene            complement(1452..2414)
                     /locus_tag="VGB68_11215"
     CDS             complement(1452..2414)
                     /locus_tag="VGB68_11215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0049850.1"
                     /translation="MERRNGLMTAGALEKIRVEIVAPVHNRRDITLQCLRSLSRLDKT
                     ALDVHIVIVDDGSTDGTSESIKSEFPEVEIVTGDGNLWFTEGTNVGIRAALKHNPKYV
                     LLINDDAVFDEKFLAFMVETAEKNPRSVVGSLLLLWDEPHKIFQVSPVWDTRSGGWRH
                     WQNQTVWSVPKKAWEVDIIVGNCVLVPAAAIEEAGLMNSKKYPNFGDVEYTPRLKKRG
                     WRLLIDPRARVFCQPNNIPASIRKLGFRKMFDALFLDLKHIHNLRRRFYGNLDSAPNR
                     WQGTLAFGIFFLRLAFGKNAESNWALNREEKPLAETFAEAQVKD"
BASE COUNT          623 a          653 c          576 g          651 t
ORIGIN      
        1 gattgccgag cgatttgacg atgatccggg tggcgaaaaa agtcaaaaca aacggcagaa
       61 tccaactcgt aaaaccgtaa aaaacgtttt tcgtgatgct tctggtcgtg gatttggtcg
      121 cttctgccat cgctcactcc gaaaaagatt cctcgtaact ttgccacgcc gtctgcgccg
      181 caattctttc gtcaaaattc tttaaaacaa actcgcttcc cgtccgcccc aatttttcgc
      241 gcaatgcttc gtcgtttttt aaagtttcaa tcgcttcgac taatgcgtcg acatcattcg
      301 gctcgattaa ccaacctgtt tccatatgtt taaccgcttc gggaatgccg ttgatatttg
      361 tggaaacgac ggctttgccg agcgccatcg cttcgagcat cgaaatcggc aagccttcga
      421 caaagctcgg caaagcgaaa acgtcagcca gacgcatcaa attaaacaaa tcgccgcgct
      481 cttttccgac ctgttcggac gtgataaaaa caaagttttc gccgagcccg taagattcgg
      541 ctttccgcaa atcttcgtca tttggttttg aattggaaat ccatacgaaa gccgcgtcgg
      601 attttttctt taaaagcttt tgcgccgctt ccagaaaaat ccagcgccct ttgcggtcga
      661 taaactgccc gacgcataaa acgaggaatt tgtcagccgg aatattaaat ttttgacgaa
      721 tcgcccgttc atctaattcg gctttttgag cgtttaataa ttcgggcgga ttgatgctgg
      781 cgtaagtcac ttttactctt tcgtaaaaat ccgccgaaac aaattcgcgc aggcattttt
      841 tcgcgtcctc gtttgccgta aatatatgaa aatttttaaa tttacttaag acgccgaatt
      901 taaatttcca aatccagtcg cgccaccact gctgcggtcg gacggaattg tggacggtcg
      961 taaaaacttt cgttttcgtc gacagccaga acaaagcggc gaaagattgc cacggtgcaa
     1021 actcgacgtg aaccgcgcag tctttcaaac cgactttttt cagataacgg cagaagacgt
     1081 attccgagcg gagcttgtta aaatgtcgcg ctaatttacg tttgacggtc aaagcgggct
     1141 ggctgtcggt gtgagaatcg acaaactcgc agggaatgcc cgcatctttt agaaatttca
     1201 gaagctgctc gtttgagcct tcgggcagaa cggcacgcac ctcgcagtgt tttttcgctt
     1261 ccttcatcaa agccagaaaa taaacctgcg cgccgcccca ttccagataa ttccagacgc
     1321 acaaaacttt tttcggcaga tgattaaaat ttccgttttc cgataacttc aagttgtttc
     1381 tcaaccgtcg gcaggcagac agttaaagta atttatgaat aaaatattag ttaaagcgat
     1441 tcttcaacag cttaatcttt tacctgagct tcggcaaaag tctccgccag aggcttttct
     1501 tccctgttta aagcccaatt gctctcggca tttttgccga aagccaggcg cagaaaaaaa
     1561 atcccgaaag cgagagttcc ctgccagcga ttcggcgcgc tgtcgagatt gccgtaaaag
     1621 cgtcgacgca aattgtgtat gtgtttcaaa tcgagaaaca atgcgtcaaa cattttccga
     1681 aaacccagtt ttctgatcga cgccggaata ttgttcggct ggcagaaaac tctggcgcgc
     1741 gggtcgatca aaagacgcca gccgcgcttt ttcaaacgcg gcgtgtattc gacatcaccg
     1801 aagttcggat attttttcga attcatcaga cccgcttcct cgattgccgc tgccggaacc
     1861 agaacgcagt tgccgacgat aatatcaact tcccaggctt ttttcggcac cgaccagacg
     1921 gtctggttct gccagtgtcg ccagccgccg gagcgcgtgt cccaaacggg cgaaacctga
     1981 aaaattttgt gcggctcgtc ccacagcaga agcagcgagc cgacgaccga gcgcggattt
     2041 ttttcggcgg tctcgaccat aaacgcaaga aacttttcgt caaacaccgc gtcgtcgtta
     2101 atcagcaaaa catatttcgg attgtgtttg agcgcggcgc gaatgccgac gttcgttcct
     2161 tcggtaaacc acaaatttcc gtcgcccgtg acaatttcga cttcgggaaa ctcgcttttg
     2221 attgattcgg aagttccgtc ggtcgagccg tcatcgacaa tgacgatatg aacgtccagc
     2281 gcggttttat cgaggcgcga caagcttcgc aggcattgca gcgtgatgtc gcgccggttg
     2341 tgaaccggcg cgacgatttc aacccgaatt ttttccagcg ctccggcggt cattaagccg
     2401 tttcgtcgct ccaaagctcg cgcaattgcc ggacgcttcc ttcgtcgccc gcgttgcagc
     2461 cgacgggaac gtaccggata ttttcgcggt tggaaagctc gac
//
LOCUS       DASWNE010000569         6393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_477767, whole genome shotgun sequence.
ACCESSION   DASWNE010000569 DASWNE010000000
VERSION     DASWNE010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6393
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477767"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..561)
                     /locus_tag="VGB68_11220"
     CDS             complement(205..561)
                     /locus_tag="VGB68_11220"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEY0049851.1"
                     /translation="MSTKLYVGKLSFRVTNDDLHEHFSQAGSVESANIVMDRETGRSR
                     GFGFVEMSTEEDANNAIAQFNGQEYDGRNMVVNEARPREEGGGGGRGGYGGGGGRGGN
                     RGGGGYGGGYGGGDRW"
     gene            complement(909..2324)
                     /gene="mpl"
                     /locus_tag="VGB68_11225"
     CDS             complement(909..2324)
                     /gene="mpl"
                     /locus_tag="VGB68_11225"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: protein motif:HMM:TIGR01081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEY0049852.1"
                     /translation="MHYHLIGICGTAMASLAGMLQARGHKVTGSDQNVYPPMSTQLES
                     LGIEIINGYKAENADVGADCTIVGNTISRGNAELEEVLNRKLLYRSQAEIVKEEFVRG
                     RRSLVVAGTHGKTTTTSIAAWVAEVGGLNPSFLVGGIVQNFGASFRVSESDFFVIEGD
                     EYDTAYFDKKPKFMHYLPEIAVVNNIEFDHADIYRDITEIKFAFSRLMNLVPGNGRLI
                     VGVDSPIAREVLDEMRGRLFTQIETFGTTDEAKWQARDIDFSGDVTRFAVFKEGAVWG
                     EFETHLIGEFNVRNCLAVIIAADAWGISKEKINEAFRTFKSVKRRVEVRGVERGVTVI
                     DDFAHHPTAVEETLKALRTKYADSRLVAVFEPRSWSSRLAVFQEPYRKAFNYADYVII
                     AGVYNTSKASELGKVLDVDELVKDIKRQNKTALAFADADAIVEHLAPELKEGDVVAIM
                     SNGGFGGIHDKILNVLKSEKG"
     gene            2599..3990
                     /locus_tag="VGB68_11230"
     CDS             2599..3990
                     /locus_tag="VGB68_11230"
                     /inference="COORDINATES: protein motif:HMM:NF021436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HEY0049853.1"
                     /translation="MSKLRLLRLPSQPQNVFLLLGAVFGLLMVFVTPPALVGDEPNHF
                     FRAYQIAGGGIIGERMENGGSGGWIPKNILDTNRKLVGEIEMNHHVKFDTNLIWELRR
                     LPVNEADTVFVPFHNTVVYAPVPYIPQVIGIYIGKVFGASPVALIYIARVINLIFFLA
                     FAYFAIRITPVHKWVFCFLWLTPTTVFQAASAAADPFMMGICFLTISYFLYHALDKDA
                     KIEKLDIVKMFVFCFLAALGKQAYVFLPFLFLAIPRRKFKSAAVYWITFFALLAICFG
                     SVAAWAYAVKPIFQSYRGDVPVNPDEQLSFVLHDPLNFLRVLYNSFVGFGGYYFVTFF
                     GGLTWLDLLLPQWHVVALFIIAVIIALLDKNEDVDIKWLHKAVFAMVCGGTVVLVSLL
                     LYLSWSPIGGDRVAGIQGRYFIPVAPLFFLLFYNYKFGWRRFTRYAWLVVYLTVVFSL
                     ALMVNSVIKRYYL"
     gene            complement(4076..5236)
                     /locus_tag="VGB68_11235"
     CDS             complement(4076..5236)
                     /locus_tag="VGB68_11235"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY0049854.1"
                     /translation="MTEAKVKKVKPDIRAITRLVPPSGNLVQGQSELPIDPRLAREAA
                     EIIAASQNHYGASEGDENLRRAVAEKIKKYNGIEIDVNAKPFQLMITNGGTGALIGIA
                     QSYLRNKSALVFEPYYPYHRRILEEFGGKTETFELDAQLTFEKDALRARCLELKNQSE
                     FPLKAIIVCSPANPSGKVMTQTELEAVSDVAQELDLLVISDEVYEHYVTGETPHIPIA
                     TLPGMWERTITVNSFSKSWNISGWRLGYAYGKGELIAPINNAANVVYVCPATPLQAAL
                     SKVLMADAEYYDNLRRKFDEKRLFVSDNLRRLGFDIYNSGSAFYLWARIPQEFSDALT
                     FNEMLMEKAGVAGTPGSAFADSDDWDAYMRICIAREDSILEGALEKLGTALK"
     gene            5463..>6393
                     /locus_tag="VGB68_11240"
     CDS             5463..>6393
                     /locus_tag="VGB68_11240"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY0049855.1"
                     /translation="MKAVAQNNLPPSVKPSLFGGHFLQFRREPTGFLQKLAALGDITS
                     FRMGRQQAFFINHPDFIRDVLVTNHSKFHKGRALKRAKKLLGEGLLTNEGESHLRQRR
                     MMQPAFHRQRINGYAETMIEFADRMADSWRDGEVRDLSQEMMHLTLQIVGKTLFNAQV
                     ADEADEIGAAMTTLIELFDYLLLPFSEILEKLPFPQMRRFDKAKRTLDKIIYGIIEER
                     RKSGEDKGDLLSMLLAARDEEGDGSGMTDEQIRDEALTLFLAGHETTANAMIFTWYLL
                     SQNAGAEAKFHAELDALFPNADAPKPQDYPRLKY"
BASE COUNT         1471 a         1682 c         1580 g         1660 t
ORIGIN      
        1 cttgaaaaga aagccggatt ccggactccg aattttaaat tctaaattgt gagctatgaa
       61 ttaagccgca taatgtttcc ccggatactt tagagtttat aattccggca gaatttagaa
      121 tcggcgcttt tgatttttcc gagcaggcaa taaaaagacc ggagccttta ccgctccggt
      181 ctttttcgtt ttttcgaatt tcgcttacca gcggtcgccg ccgccgtagc cgccgccata
      241 accgccgccg ccgcgattgc cgccgcgacc gccgccgcca ccgtaaccgc cgcgtccgcc
      301 accgccgcct tcttcgcgcg ggcgggcttc gttgacgacc atattacgac cgtcatattc
      361 ctgcccgttg aattgggcga tagcgttgtt ggcgtcttct tccgtgctca tttcgacaaa
      421 tccgaaaccg cgcgagcgtc cggtttcgcg gtccatcacg atatttgccg attcaaccga
      481 gccggcttgc gaaaagtgtt catgtaaatc atcgttcgtc acgcgaaacg acaactttcc
      541 gacgtatagt tttgtactca ttttctctct ttattattta tttaaccagg gaagcaaaag
      601 gaagggcgaa tggcacgaaa aattctcaaa atattttctt tccaataggg cgacgcgccc
      661 accttgtcac gataaaactc agactgcttc aaaaccactt atccaactct ccaaccgacc
      721 cgaaacgtcg ctaatcttat cgtgtacgga cgctatctac gcagatacct gagagcaaaa
      781 acctcagtca gtatgcctct ttttgacgtt ttaggcaaga gaaaagggta aaagtgaaaa
      841 agggtaaaag tgaaaaagta aattgaattt tactcgacaa actttttcgc cttttcactt
      901 ttaccctctt aacctttttc acttttcagg acgttcaaaa ttttgtcgtg aatgccgccg
      961 aagccgccgt tggacataat cgctaccacg tcgccttctt ttaattccgg cgcgagatgc
     1021 tcgacaatcg cgtcggcgtc cgcgaaggcg agcgcggttt tgttttgccg tttgatgtct
     1081 ttgaccagct cgtcgacatc caaaacctta ccgagctcgc ttgctttcga cgtgttgtaa
     1141 acgcccgcga ttatcacgta atccgcgtaa ttaaaggctt ttctataagg ctcctgaaaa
     1201 accgccagcc gcgaagacca ggaccgcggc tcgaaaacgg cgaccaggcg gctgtctgcg
     1261 tatttcgttc tcaaggcttt taaagtttct tcgaccgccg tcggatgatg agcgaaatcg
     1321 tcaatcacgg tcacgcctct ttcgacgccc cgcacttcga cgcggcgctt gacggattta
     1381 aaagtgcgaa aagcttcgtt tattttttct ttggaaattc cccaggcgtc agccgcgata
     1441 attaccgcca ggcagtttcg cacattgaat tcgccgatga ggtgcgtttc aaattcgccc
     1501 cagacggctc cttctttgaa aaccgcaaaa cgcgtcacgt cgcccgaaaa atcaatatct
     1561 cgcgcctgcc acttcgcttc gtccgttgtt ccgaaagttt cgatttgcgt aaaaagcctg
     1621 ccgcgcattt cgtccagaac ttcgcgcgca atcggcgaat cgacgccgac aatcaggcgc
     1681 ccgttgcccg gcaccagatt catcaggcgc gaaaaggcaa acttgatttc cgtgatgtcg
     1741 cgataaatgt cggcgtggtc gaattcgatg ttgttgacga cggcgatttc cggcagataa
     1801 tgcataaact tcggcttttt atcaaaataa gccgtgtcgt attcgtcgcc ctcgataacg
     1861 aaaaaatcgc tttccgaaac gcgaaagctc gcgccgaaat tctgcacgat gccgccgacc
     1921 agaaacgacg gattcagccc gccgacttcc gcgacccacg cggcgatgct cgtcgtcgtc
     1981 gtcttcccgt gcgttcccgc cacgaccaga cttcgacgcc cgcgaacgaa ttcttccttg
     2041 acgatttccg cctgcgagcg atacaaaagc ttgcgattta aaacttcctc aagctccgcg
     2101 ttgccgcgcg aaatcgtgtt gccgacaatc gtgcaatcgg cgccgacatc ggcattttcc
     2161 gccttgtagc cattgataat ttctattccg agcgattcta attgcgtcga catcggcggg
     2221 taaacattct ggtctgagcc cgtaacttta tgcccgcgcg cctgcagcat tcccgcaagc
     2281 gaagccatcg ccgtgccgca gattccgatt aaatgataat gcataaaaat aagttattgt
     2341 ttcgagaaag gcgtataatt tacgagcatt cgattgctcc ggtctgcctt aaacagcaac
     2401 ctctataaat tttcagacac aaagccgaaa aaagcaaaat gtctttggat aatccggcaa
     2461 aactccagta gaatcgccta aactgcaaaa actgatatta aagacttaac tattgaggcg
     2521 ggtgttttga gccgagcctt ttaaaattta gcggttaatt actgattttt tatcgaccgt
     2581 ataatgagca ctgagaaaat gtcgaaatta cgcctcttgc gtctgccttc ccaacctcaa
     2641 aacgtattcc ttttgctggg tgcggttttc ggtctgctga tggttttcgt cacgccgccg
     2701 gcgctggtcg gcgacgagcc gaatcatttt tttcgcgctt atcaaatcgc gggcggcgga
     2761 attatcggcg aaagaatgga aaacggcggc agcggcggct ggataccgaa aaacatcttg
     2821 gacaccaacc gaaaactggt cggcgagata gaaatgaatc atcacgtcaa gttcgacacg
     2881 aacctgattt gggagctgcg ccggctgccg gtcaacgaag cggacacagt gtttgtgcct
     2941 tttcataata cggtcgtcta cgcgcccgtc ccgtatattc cgcaggtcat cggcatctac
     3001 atcgggaaag ttttcggcgc atcgcccgtg gcgctcatct atattgcgcg cgtcattaat
     3061 ttgatctttt ttttagcttt tgcctatttc gccatcagaa taactcccgt tcataaatgg
     3121 gtgttctgtt ttctctggct gacgccgacg accgtttttc aggcggcttc ggcggctgcc
     3181 gacccgttta tgatgggaat ctgttttctg acgatttcct attttctgta tcacgctctg
     3241 gataaagacg cgaaaatcga aaagctcgac atcgttaaaa tgtttgtttt ctgttttctc
     3301 gccgcgctcg gcaaacaagc gtatgttttc ctgccttttc tgtttctggc gattccgcgc
     3361 cgcaagttca aatcagccgc cgtttactgg attacttttt tcgcgctgct ggcgatttgt
     3421 ttcggcagcg tcgccgcctg ggcgtatgcc gtaaaaccta tttttcaatc ctatcgcggc
     3481 gatgtgccgg taaacccgga cgagcagtta agcttcgttc tgcacgaccc gcttaacttt
     3541 cttcgagtcc tttacaacag cttcgtcgga ttcggcggct attatttcgt gacgtttttc
     3601 ggcggcttaa cctggctcga tttactcttg ccgcaatggc acgtcgttgc tctgtttatt
     3661 atcgccgtta tcatcgccct gctcgataaa aatgaagacg tcgatataaa atggctgcat
     3721 aaagccgtct ttgcaatggt ctgcggcggc acggtcgttc tggtcagcct gctgctgtat
     3781 ttatcctggt cgccgatcgg cggcgataga gtcgccggaa ttcagggaag atattttatt
     3841 ccggtcgcgc ccttgttttt tctgcttttt tataattata aattcggctg gagaagattt
     3901 acccgttacg cctggctggt cgtgtatctg acggtcgttt tttcgctcgc gctgatggtc
     3961 aacagcgtga taaagagata ctacctttaa agagatttga ataaataaag aggtttgaat
     4021 aaaaaaaatt acggttttgt aaaattcctt ctatccgaaa cctcttaatt tttatttatt
     4081 tcaaagccgt tccgagtttt tccagcgcgc cttccagaat cgaatcttcg cgggcgatgc
     4141 agatgcgcat atatgcgtcc caatcgtcgg aatcggcgaa ggcgctgccg ggcgtgcccg
     4201 cgacgcctgc tttttccatc agcatttcgt tgaaggtcag cgcatcgctg aattcctgcg
     4261 gaatccgcgc ccagaggtag aaagccgagc cggaattgta aatatcgaag ccgagccgcc
     4321 gcagattgtc ggaaacgaac agacgttttt cgtcgaattt gcggcgcaga ttgtcgtaat
     4381 attcggcgtc cgccattaga actttcgata aagccgcctg cagaggcgtc gccggacaga
     4441 cgtagacgac gtttgccgcg ttatttatcg gcgcgataag ctcgcctttg ccgtaagcgt
     4501 agccgagtcg ccagccggaa atgttccacg atttcgaaaa tgaattgacc gtaatagtgc
     4561 gctcccacat tccgggcagc gtcgcgatgg gaatatgcgg cgtttcgccc gtcacgtaat
     4621 gctcgtaaac ctcgtcggaa atgacgagca aatcgagttc ctgcgcgaca tcggagacgg
     4681 cttcgagctc ggtttgcgtc atcactttgc cgctcgggtt ggcgggcgag cagacgatga
     4741 ttgctttgag cggaaattcg ctttgatttt tcagctccag acagcgggcg cgcagggcgt
     4801 ctttttcaaa agtcaattgc gcgtccagct cgaaagtttc ggtcttgccg ccgaattctt
     4861 ccagaatgcg ccggtgatac ggataatacg gctcgaaaac aagcgcggat ttatttctca
     4921 gatagctttg cgcgatgccg atgagcgcgc ctgttccgcc gttggtaatc atcagctgga
     4981 acggcttggc attaacgtca atctcgatgc cgttgtattt tttaatcttt tcggcgacgg
     5041 ctcggcgcaa attttcgtcg ccttcgctcg cgccgtaatg attctggctg gcggcgatga
     5101 tttcggcggc ttcccgggcg agccgcgggt cgatcggcag ctccgattga ccttgcacga
     5161 gattgccgct cggcggcaca aggcgcgtaa tcgcgcgaat atcgggtttt acttttttca
     5221 cttttgcttc tgtcataagt tataaaaata tcagttttgc gcggaaaatt ctaaacgcgc
     5281 aagcagaaaa aataaaaccg cgaataaagg cggagccgcg cgggttagaa gaatcgaccg
     5341 tcggataaag gcgaccggca gagataaaag aaagcagtta aaatacctct gttcatctgc
     5401 ttcgtccgag gttgattcct ttgtatttat ccgtggacaa tttctctgtt aatatcgagt
     5461 cgatgaaagc agtagcgcaa aataatctgc cgccttcggt caagccttcg cttttcggcg
     5521 ggcattttct gcaatttcgg agagagccca ccggatttct gcaaaagctt gccgcgctcg
     5581 gcgatattac gtcttttcgg atgggcaggc agcaggcgtt ttttatcaat cacccggatt
     5641 ttatccgcga cgttctggtc acaaatcatt ccaaatttca taaagggcgc gctttgaaac
     5701 gcgcgaaaaa actgctgggc gaaggcttgc tgaccaacga gggcgaatct cacctgcgcc
     5761 agcggcggat gatgcagccc gcgtttcatc gccagcgaat caacggatat gccgaaacga
     5821 tgattgaatt cgccgaccgg atggcggatt cgtggcgcga cggcgaagtg cgcgacctct
     5881 cgcaggaaat gatgcacctg actttacaaa tcgtcggcaa gacgcttttc aacgcgcagg
     5941 tggcggacga agccgacgag atcggcgcgg cgatgacgac gctaatcgag cttttcgatt
     6001 acctgctttt gccgttttcg gaaattctgg agaagctccc gtttccgcag atgcgcaggt
     6061 ttgataaggc gaagcggacg ctcgacaaaa tcatttacgg aattatcgaa gagcgccgaa
     6121 aatcgggcga agataaaggc gatttgcttt cgatgctgct cgccgcgcgc gacgaggaag
     6181 gcgacggctc gggaatgacc gacgagcaaa tccgcgacga agcgctgacg ctttttctcg
     6241 ccggacacga aacgaccgcc aacgcgatga ttttcacgtg gtatctgctc tcacaaaacg
     6301 cgggagcgga agcgaaattt cacgccgagc tcgacgcgct ttttccgaac gccgatgcgc
     6361 cgaaaccgca ggattacccg cgtctgaaat aca
//
LOCUS       DASWNE010000570         5814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_654941, whole genome shotgun sequence.
ACCESSION   DASWNE010000570 DASWNE010000000
VERSION     DASWNE010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5814
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654941"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..790)
                     /locus_tag="VGB68_11245"
     CDS             complement(104..790)
                     /locus_tag="VGB68_11245"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0049856.1"
                     /translation="MNQNILIIEDEPDIAESLRYNFERDGFKAQIAESGEKGLRLALN
                     DAGAPSLIILDLMLPGMSGMELCRRLRREPQTRKTPIIMLTAKAAEADKIAGLELGAD
                     DYIVKPFSVKEVLARVRAVLRRTDESSIQRYEDDKLSIDFEDMRVTCSGQQVKLTRKE
                     FALLTNLAKNAGRVATRQNLLDNVWGYSYFGDTRTLDVHIRRLRQKLGDCADCIETVV
                     GVGYRFIGMK"
     gene            complement(938..1579)
                     /gene="phoU"
                     /locus_tag="VGB68_11250"
     CDS             complement(938..1579)
                     /gene="phoU"
                     /locus_tag="VGB68_11250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEY0049857.1"
                     /translation="MERELDILRDRLLLMGGEVELALQRAMHALTHRDSEVANQVLEN
                     DRVIDRMELEIDRLSVEVLALRRPVARELRTVISISKITPILERIADHCSSIARAAIE
                     LNDEPELKTFGELEEMAQRAAEMLQSALDAFTSNDSEKARALIPMDAEVDRIYERYFN
                     DLMQTMSQDGAKAARLARLLFVAKNLERIGDYVKDICELTVYMREAVFIKHTG"
     gene            1717..1917
                     /locus_tag="VGB68_11255"
     CDS             1717..1917
                     /locus_tag="VGB68_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049858.1"
                     /translation="MENGKREMESGKRRLQAFGRYSEINKQSLEAAERRLEVAGRRLE
                     AYKSRLEVRKCDFLTVKRRFPS"
     gene            complement(1993..2499)
                     /locus_tag="VGB68_11260"
     CDS             complement(1993..2499)
                     /locus_tag="VGB68_11260"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY0049859.1"
                     /translation="MKTLFILRHAKSSWDNPDLADFERPLNERGLRAAPFVGNLMRER
                     GLRVDLILSSPARRAGQTATMVKEAARFDAAIRFDERIYEASPISLLYLLAETEDQFD
                     SILLVGHNPGMEGLIKILSGGILSMPTAALAQIELNVDFWSKIAAESGKLSFVVRPKD
                     EMKRFGAD"
     gene            2713..3627
                     /locus_tag="VGB68_11265"
     CDS             2713..3627
                     /locus_tag="VGB68_11265"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049860.1"
                     /translation="MKTQTIAAIDIGSNSIKLAVAEAGGDCLNFIFQERERVRMGDTL
                     REKLLSPEAVEKSAAAVLTFRAIAESLQAETIIAVATASAREAINSGAFVGEIERQTG
                     VRVKVLAAIEEARLIGLAARHYFRAATGALLNVDIGGGSTELSLMKTGAPERLFSMPL
                     GALRLTGKFLSANPPTEKEIENVRGEIALNLEKPAEQLSVENWRLTTGTSGTILKFER
                     LLNSENGEICLAQLTELNRKLAKMNVAERARLPNISEKRAETIVAGGLILEGIMRALK
                     VEKLQPCEYALREGVIIDFLESNLKIAG"
     gene            complement(3624..4493)
                     /locus_tag="VGB68_11270"
     CDS             complement(3624..4493)
                     /locus_tag="VGB68_11270"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HEY0049861.1"
                     /translation="MKYEQVRIREGKKLNLKKHPTDFTGDYRDKAEAKADLARNVERL
                     AELQEVLYAQNKYALLIVLQAMDAAGKDGLISHVMSGINPQGVQVVSFKQPSAEELEH
                     DFLWRISKNLPERGKIGVFNRSHYEDVLVVRVHPEIIQSQPLPDEVKNDKNFWEKRFE
                     QIRSFEKHLVENGTHILKFFLHLSRDEQKKQFLERIDTPEKNWKFVDSDLTERGFWDD
                     YMRAYEEAIAATSTDDAPWYVLPADKKWFTRTAASEIIRAKLESMDLRYPTVSDERIK
                     RLREAKKVLENET"
     gene            4752..5456
                     /locus_tag="VGB68_11275"
     CDS             4752..5456
                     /locus_tag="VGB68_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049862.1"
                     /translation="MLKSLLVFTFAGAFLSACPSTETVESTRVPAASVYQSYSISASK
                     RQTSVYAVFRLANETGKTIDLDAPAKIDLNDQPMTEIAPTFASGTTYEAKSGGFVPKQ
                     RFAFTDAGGKVYRNEIALQALEIPIQKIVLSRTKAALVRLSRGVAPDERISVSVTGKA
                     LADKTPLNKTVEASLDESRVNLSVAPQSLKEFMTGAAKISVMVEKKESLKQATPSGGA
                     MRFVYQSNEAASRITK"
     gene            5572..>5814
                     /locus_tag="VGB68_11280"
     CDS             5572..>5814
                     /locus_tag="VGB68_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049863.1"
                     /translation="MKKIFCFFGLSLFLSVSNFAQVKLVPHKITLKNGKSFSLNLPAD
                     YEIVPAVEGLKRVRFFAKSPDGRIFVTDMYNLTDNTK"
BASE COUNT         1393 a         1519 c         1492 g         1410 t
ORIGIN      
        1 agccgtcagc agacagctat ttgccttgcc tgcgataccc ggcttcatcc aaccggcaag
       61 catcagcagg cagctgacgg ctgaacgctg acggctgata gctctatttc atcccgataa
      121 agcgatagcc gacgccgacg accgtctcga tgcagtcggc gcagtcgccg agcttttgcc
      181 gcagccggcg aatatggacg tccaatgtcc gcgtgtcgcc gaaatagctg tagccccaga
      241 cgttatcgag cagattctgc cgcgtcgcga cgcgtccggc attttttgcc agattggtca
      301 aaagggcaaa ttccttgcgc gtcagcttta cctgctgacc gctgcacgtg acgcgcatat
      361 cttcgaaatc aatcgaaagc ttgtcgtctt cataacgctg aatcgagctt tcgtccgtcc
      421 ggcgcaaaac ggcgcggacg cgcgccagaa cttccttgac cgagaaaggc ttgacgatat
      481 aatcgtccgc gccgagttcc agaccggcga ttttatcggc ttcggcggct ttcgccgtga
      541 gcataattat cggcgttttg cgcgtttgcg gctcgcggcg caaacggcgg caaagctcca
      601 tcccgctcat tcccggcagc atcaaatcca gaataatcag cgaaggcgcg cccgcgtcgt
      661 tcagagccag ccgcaagcct ttctcgcccg attcggcaat ctgcgccttg aaaccgtcgc
      721 gctcgaaatt ataccgcagg ctttcggcaa tatccggctc gtcttcgatg attagaatgt
      781 tttggttcat agattcagga ttcaggattc aggattcagg attcaggatt caggattcag
      841 gattcaggat tcaggattca ggatttaaga ttgaaggaaa attcccaatc ctgaattttg
      901 aatcctgaat cctgaattcc caatcctgaa tcctgaatta tcccgtatgt ttgataaaaa
      961 ccgcctcgcg catatagacg gtcagctcgc aaatgtcttt gacgtaatcg ccgattcttt
     1021 ccagattttt ggcgacgaac agaagccgcg ccagccgcgc cgctttcgcg ccgtcctgcg
     1081 acatcgtctg catcaaatcg ttaaaatatc gctcgtaaat acggtcgact tccgcatcca
     1141 tcggtatcag cgcgcgcgct ttttccgaat cgttcgacgt aaaagcgtcg agcgccgatt
     1201 ggagcatctc ggcggcgcgc tgcgccattt cttccagctc gccgaaggtt ttcagctccg
     1261 gctcgtcgtt cagttcgatg gcggcgcgcg cgatactcga acagtggtcg gcgatgcgtt
     1321 cgagaatcgg cgtgattttt gaaatcgaaa tcacggtgcg caattcgcgc gctaccggtc
     1381 ggcgcagcgc cagaacttcg acgctcaggc ggtcgatttc cagttccata cggtcaatca
     1441 cgcggtcgtt ttccaaaacc tgattggcga cttcgctgtc gcgatgcgtc agcgcgtgca
     1501 tcgcgcgctg caaggcaagc tcgacctcgc cgcccattaa aagaagacgg tcacgtaaga
     1561 tgtccagttc gcgttccaaa agttttctct gctccacaat tcaagacttt agaacaaaac
     1621 tgcgaattaa gaaagagatt gcgcgcccga tactcaagca gagtattacg cgaaactgaa
     1681 cataagatga atagattgtt aaaaagatgt taattaatgg aaaatggaaa acgggaaatg
     1741 gaaagcggaa aacgccgttt gcaagctttc ggacgatatt cggagattaa caaacaaagt
     1801 ttggaagctg ccgaacgccg tttggaagtt gccggacggc gtttggaagc ttacaaatcg
     1861 cgtttggaag ttagaaaatg tgattttctt actgtcaaac gccgttttcc ttcttaaaat
     1921 caaaaatttt gtatgagatt gatgcaagtg gaataagcgc aaacgcggct ttccggtttg
     1981 ccttctgctt ttttagtctg cgccgaaccg tttcatctcg tctttcgggc gcacgacgaa
     2041 actcagcttt ccgctctcgg cggcgatttt gctccagaaa tcgacgttca gctcgatttg
     2101 cgccagcgcc gccgtcggca tcgacaaaat tccgccgctc agaattttta tcaagccttc
     2161 cattccggga ttgtgaccga ccagcagaat cgaatcgaat tggtcttccg tttcggcgag
     2221 caggtaaaga agcgagatgg gactggcttc gtaaattctt tcgtcgaagc gaatcgcggc
     2281 gtcaaaccgg gcggcttcct tgaccatcgt cgccgtctgc ccggcgcgtc tggcgggcga
     2341 gcttaaaatc aaatcgacgc gcaagcctct ttcgcgcatc agattgccga caaacggcgc
     2401 ggctcgtaat ccgcgctcgt ttaaaggacg ttcaaaatcc gctaaatccg gattgtccca
     2461 gcttgatttg gcgtggcgca aaataaaaag cgttttcata gtaaattgaa gaaaccggcg
     2521 cagcgggcga gcgtcttccg aaaaatgaat ttttagccgt ttgccgaaag aagtcagcgc
     2581 ggacgaaccc ggaccgggtt tgtcccgaaa agaataacag taaatttcac gattttgtag
     2641 tcttaaattt aatttttcat aaaaaataaa tcggtattta tcggcggcgg aaaattgaat
     2701 tatcatcttc caatgaagac tcaaaccatc gccgccatcg acatcggttc aaattccata
     2761 aaactcgccg tcgccgaggc gggcggcgat tgcttgaatt ttattttcca ggagcgtgaa
     2821 agagtgcgga tgggcgacac gctgcgcgaa aagcttcttt caccggaagc cgtcgaaaaa
     2881 tccgccgccg ccgtgctgac gtttcgcgcg attgccgaaa gccttcaagc cgaaacgatt
     2941 atcgccgtcg ctacggcgtc ggcgcgcgag gcgataaatt ccggcgcttt tgtcggcgaa
     3001 atcgagcggc aaacgggcgt tcgcgtcaag gttctggcgg caattgagga agcgcgattg
     3061 atcggactcg ccgcgcggca ttattttcgc gcggcgacgg gcgcgctttt aaacgtcgac
     3121 atcggcggcg gctcgaccga attgagcctg atgaaaacgg gcgcgccgga aaggcttttc
     3181 tcgatgccgc tcggcgcgct ccgtttgacc ggaaaatttc tttccgcaaa tccgccgacc
     3241 gaaaaagaaa tcgaaaacgt gcgcggcgaa atcgcgctca atctggaaaa acctgccgaa
     3301 caattaagcg tcgaaaactg gcgtctgacg accggaactt ccggcacgat tttaaaattc
     3361 gaacggcttt taaattctga aaacggcgaa atttgtttgg cgcagcttac cgaattaaac
     3421 cggaaactgg cgaaaatgaa cgtcgccgag cgcgcccgct tgccgaacat cagcgagaaa
     3481 cgcgccgaaa cgatcgtcgc gggcggactg attctcgaag gcattatgcg cgctctgaaa
     3541 gtcgaaaagc tccagccgtg cgaatacgct ctgcgcgaag gcgtcattat cgactttctc
     3601 gaaagcaatt tgaaaatcgc gggttaagtt tcgttttcca gaaccttttt cgcttcgcgc
     3661 aggcgtttta tgcgctcgtc ggagacggtc gggtatcgca aatccatcga ttccagcttg
     3721 gcgcggataa tctcggacgc cgccgtgcgc gtaaaccatt ttttgtctgc cggaagcacg
     3781 taccagggcg cgtcgtcggt cgaggtcgcc gcaatcgctt cttcgtaggc gcgcatataa
     3841 tcgtcccaga agccgcgctc ggtcaaatcg gaatcgacaa atttccaatt cttttccggc
     3901 gtgtcgatgc gttcgagaaa ctgttttttc tgttcgtctc tcgacaggtg cagaaaaaat
     3961 ttcagaatgt gcgtgccgtt ttcgaccaga tgtttttcaa acgagcgaat ctgctcgaag
     4021 cgtttttccc agaagttttt atcgtttttc acttcgtccg gcagcggctg cgattggatg
     4081 atttccggat gcacgcgcac caccagaacg tcttcgtaat gcgagcgatt aaaaacgccg
     4141 atttttccgc gctccggcag atttttggaa atccgccaca gaaaatcgtg ttcgagctct
     4201 tccgccgaag gctgtttgaa cgaaacgacc tgcacgccct gcgggttgat gccgctcatc
     4261 acgtggctga tcaagccgtc tttgccggcg gcgtccatcg cctgcaaaac gatgagcagc
     4321 gcgtatttgt tctgcgcgta gagaacttcc tgcaattcag ccagccgctc gacgttgcgc
     4381 gccaaatccg cttttgcctc ggctttatcg cggtaatcgc ccgtaaaatc ggtcggatgt
     4441 tttttaagat ttagtttttt gccctcgcgg atgcggactt gttcgtattt cataaaaggt
     4501 ttattcaact ttaagttttt tccgcgtaaa aacacaagta aaaagtttga gccgtttcgc
     4561 gccggaaaac tgcgtttaca gacagggaaa tcttcgcaaa atttccgccc gcggcagtca
     4621 attgttatcg gataaaaaat ttatgaaaaa caaccgggaa acaaactttc agacaagcgg
     4681 cgtccggctg atgatttatg cggcggcgat gcttccggtg atgattttcg tgaataaata
     4741 ttaagaaaat tatgttaaaa agtcttctgg tttttacttt tgccggcgct tttctgtcag
     4801 cctgcccgtc aaccgaaacg gttgaatcga cgcgcgttcc ggcggcgagc gtttaccaga
     4861 gctattcgat ttccgcttcg aaacgacaaa cgagcgtcta cgcggttttt cgcctcgcta
     4921 atgaaacggg caagacgatt gatttggacg cgcccgccaa aatcgattta aacgaccagc
     4981 cgatgacgga aatcgcgccg actttcgcga gcggcacgac gtacgaagcc aaatcgggcg
     5041 gttttgtacc gaagcagcgg tttgctttta ccgatgccgg cggtaaagtc taccgcaatg
     5101 aaatcgcgct tcaggctctg gaaattccaa ttcagaaaat cgttttgagc cggactaagg
     5161 cggcgctcgt caggctctcg cgcggcgttg cgccggacga gcggatttcc gtctccgtga
     5221 ccggaaaagc cctcgccgat aaaactccgc tgaacaagac ggtcgaagca agtctggacg
     5281 aatcacgcgt caacctctcg gtcgcgccgc aaagcttgaa ggaatttatg acgggcgcgg
     5341 cgaagattag cgtgatggtc gagaaaaaag aatcgctcaa acaggcaacg ccgagcggcg
     5401 gcgcgatgcg atttgtttat caatccaacg aagcggcgtc gagaattaca aaataatctt
     5461 gcaaaaaaca aattttattc gtttaatatc ggaaaatcac tttgcgcgtt tcgcgccccg
     5521 ggatttttgc aaagccgctc tgtttttgag cctgcagggc gctgaaattt tatgaaaaag
     5581 attttttgtt tcttcggttt atcgcttttt ttatccgttt cgaattttgc gcaggtgaaa
     5641 ctcgttccgc ataaaatcac gttaaaaaac ggcaaatcct ttagcttgaa cctgcccgcc
     5701 gattacgaaa tcgttcccgc cgtcgagggc ttaaagcgcg tccggttttt cgccaaatcg
     5761 ccggacgggc ggattttcgt gaccgatatg tataacctga ccgacaacac taag
//
LOCUS       DASWNE010000571         3471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_267106, whole genome shotgun sequence.
ACCESSION   DASWNE010000571 DASWNE010000000
VERSION     DASWNE010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267106"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VGB68_11285"
     CDS             <1..642
                     /locus_tag="VGB68_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049864.1"
                     /translation="KSYRITRLLAKNGRALSAGDQADEDKKVEKQIREIEERIAQKEK
                     KQLERERDIAADKTGVPDEEGEGQRITLSDALRGSLLVNPRRERLRGRDVIVFDYEPN
                     PAYKPKTRFERVFALCSGAVWVDADDKQIVRLEASLTQSSGNFLAKLKRGASFTLENE
                     RVNDEIWLPSLADINLSVKILFAGINVNNLIRYGDYKRFDTEVKDGKVNEIKP"
     gene            complement(731..1813)
                     /locus_tag="VGB68_11290"
     CDS             complement(731..1813)
                     /locus_tag="VGB68_11290"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEY0049865.1"
                     /translation="MSAKFKAEAVSNSQSKTNLKRLRWIGAVFTILGIALFSYFIASV
                     GFHEVMRGIARIGFDGFAIIILIYFSRILIRAASWKLSVSEPYSLTLRDTTEGVIIGE
                     AMSSIIPLGIMVSGTSKAISVRKKMPIVVALSTVATENLFYSLVTGLFIVFGAVVFLR
                     NFELSESWTLMLNVLIGFVFLLIILGVLMVVRQWHFLSAILERLYAKEILRRWTEDGR
                     LHARLFENLIYGFYRQYPKRFLPIFALQILFHVLGVFEVWFILNRLSDAATVYSAFLL
                     ESISRVITVVFKLIPFLIGVDEAGAQFVTETLALGAGLGVTLAIVRKGRIIFWAIVGV
                     ALILKRGLSFSDIHKANEDLEAKTQV"
     gene            1870..3051
                     /locus_tag="VGB68_11295"
     CDS             1870..3051
                     /locus_tag="VGB68_11295"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY0049866.1"
                     /translation="MNATALEKEILNPALTQLSEEEELFRSSVREFAEGEVRPRVEKM
                     EHESKLDSELIKQCFELGLMAIETPEEYGGAGSSFFNAILAIEELARVDASTSVFVDV
                     QNTLVTNAIIRWASEAQKLKYLPQMAESKVGAYALSEAASGSDAFALKTRAVEKDDFY
                     EITGQKLWITNGNEAEIFVVFATVDPNAGYKGITAFIVEKGFEGFSVGKKEDKLGIRA
                     SSTTELILDNCRVPKENVLGEVGKGYKVSIETLNEGRIGIGAQMIGIAQGAYEAALRY
                     TAEREQFGNALNNFQAVQFQLAEMAIDVEAARLLVYNAARLKDAGKSFLKEAAMAKVY
                     SSRVAERVSSKAIELFGGYGYVKDYPVEKFWRDSKIGAIYEGTTNMQLQTIAKLITSG
                     K"
     gene            3202..3402
                     /locus_tag="VGB68_11300"
     CDS             3202..3402
                     /locus_tag="VGB68_11300"
                     /inference="COORDINATES: protein motif:HMM:NF021558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2281 domain-containing protein"
                     /protein_id="HEY0049867.1"
                     /translation="METYTVEILEPKAKKLLDDLANLNLIKLQKTEKPEQKKRKFGSM
                     KGLVKHIAEDFDAPLEDFKDYM"
BASE COUNT         1006 a          853 c          866 g          746 t
ORIGIN      
        1 aaaagctacc gaatcacgcg cctgctggcg aaaaacggca gggctttgag cgcgggcgac
       61 caggcggacg aagacaaaaa ggtcgagaaa caaattcggg aaatcgaaga aagaatcgcg
      121 caaaaggaaa agaaacaact ggaacgagag cgcgacatcg ccgccgacaa aaccggcgtg
      181 cccgacgagg aaggcgaagg tcagcgcatc acgctgtcgg acgccctgcg cggctctctg
      241 ctcgtcaatc cgcgccgcga gcgcttgcgc gggcgcgacg tgattgtctt tgattacgag
      301 ccgaatccgg cttataaacc gaaaacgcgt tttgaaaggg ttttcgcgct gtgcagcggc
      361 gcggtctggg tcgatgccga cgataaacaa atcgtcaggc tcgaagcttc gctcacgcag
      421 agctccggca attttctggc aaagctcaaa cgcggcgcgt ctttcacgct cgaaaacgag
      481 cgcgtcaacg acgaaatctg gctgccgtcg ctggcggaca taaatctatc ggtcaagatt
      541 ctgtttgccg gaatcaacgt caataattta atcagatacg gcgactataa acgcttcgac
      601 accgaagtca aagacggcaa ggtgaatgaa ataaaaccgt gaaaaacggt aaatggtaag
      661 tggtaagttg caataacaaa cttaccactt accgtttacc actttttacc gtttaccgtt
      721 taccactttt ttacacttgc gttttcgctt ccaaatcttc gttcgccttg tgaatatcgc
      781 tgaaggaaag cccgcgtttg agaatcaaag ccacgccgac gatcgcccag aaaataatcc
      841 ttcccttgcg cacaatcgcc aatgtcacgc ccaagcctgc gccgagcgcg agcgtttcgg
      901 tcacgaattg cgcgccggct tcgtcgacgc cgatgagaaa cggaatgagc ttgaaaacga
      961 ccgtaatgac gcggctgatc gattcgagca gaaaagcgct gtaaacggtc gccgcgtcgc
     1021 tcaggcgatt taagataaac cagacttcga agacgccgag cacgtgaaac aaaatttgca
     1081 gcgcaaaaat cggcagaaat cttttcgggt attgacggta aaaaccgtaa atcagatttt
     1141 cgaaaaggcg cgcgtgcagc cgtccgtcct cggtccagcg gcgcaaaatt tcctttgcgt
     1201 aaagacgttc cagaatcgcg ctcaaaaaat gccactggcg aacgaccatc aaaacgccga
     1261 gaattatcag caggaaaaca aaaccgataa gaacgttcag catcagcgtc cagctttccg
     1321 aaagctcgaa attgcgcaga aaaaccaccg cgccgaagac gataaaaagt ccggtcacga
     1381 gcgaataaaa aagattttcc gtcgccaccg tcgaaagagc gacgacaatc ggcatttttt
     1441 tgcggacgga gatggctttc gacgtgccgc tgaccataat gccgagcgga atgatgctgc
     1501 tcatcgcctc gccgataatc acgccttccg tcgtgtcgcg caaagtcaat gaataaggct
     1561 cggaaaccga taatttccag ctcgcggcgc gaatcagaat ccgcgaaaaa taaattaaaa
     1621 tgataatcgc aaaaccgtcg aagccgatgc gcgcgatgcc gcgcataact tcgtgaaaac
     1681 cgaccgaggc gatgaagtag gaaaaaagcg caattcccag aatcgtaaaa accgctccaa
     1741 tccagcgaag ccgcttcagg tttgttttgg attgagaatt tgaaactgct tctgctttga
     1801 actttgcact cattactttt atgatgttat ttcatttaca tttttttaca aaatttaaag
     1861 gaaattatta tgaacgcgac agctttagaa aaagaaattt taaatcccgc tttaacgcag
     1921 ctttccgaag aagaagagct tttccgctca agcgtgcgcg agtttgccga aggcgaagtg
     1981 cgcccgcgcg tcgagaaaat ggagcacgaa tccaaattag attcggagtt aatcaaacag
     2041 tgttttgaac tcggtttaat ggcaatcgaa acgccggaag aatacggcgg cgcgggttct
     2101 tcgtttttca acgccatttt agcgattgaa gaactggcgc gcgtcgacgc ttcgacctcg
     2161 gtttttgtcg acgtgcagaa cacgctcgtc accaacgcga ttattcgctg ggcaagcgag
     2221 gcgcagaaat tgaaatacct gccgcagatg gctgaatcga aagtcggcgc ttacgctttg
     2281 tccgaagccg cttccggctc cgacgctttc gccctgaaaa cgcgcgccgt cgaaaaagac
     2341 gatttttacg aaatcaccgg gcagaaactc tggattacca acggcaacga agccgaaatt
     2401 ttcgtcgtct tcgcgaccgt cgacccgaat gcgggctaca aaggcatcac ggcatttatc
     2461 gtcgaaaaag gcttcgaagg cttttcggtc ggcaaaaaag aagacaagct cggcatccgc
     2521 gcgtcttcga cgacggaatt gattctggac aactgccgcg tgccgaagga aaacgttctc
     2581 ggcgaagtcg gcaaaggcta caaggtttcc atcgaaaccc tgaacgaagg tcgcatcggc
     2641 atcggcgcgc agatgatcgg catcgcgcag ggcgcatatg aagccgcgct gagatacacg
     2701 gcggaacgcg agcagttcgg caacgcgctc aataactttc aagccgtcca gtttcagctc
     2761 gccgaaatgg cgattgacgt cgaagccgcg cgcctgctgg tttacaacgc cgcgcgtttg
     2821 aaagacgccg gaaaatcttt cctgaaagaa gcggcgatgg cgaaagttta ttcctcgcgc
     2881 gtggcggaac gagtttcgtc aaaagccatc gagcttttcg gcggctacgg ctacgtcaag
     2941 gattatccgg tcgaaaaatt ctggcgcgac tcgaaaatcg gcgcgattta cgaaggcact
     3001 acgaatatgc agctgcagac gatcgccaaa ctgattacga gcggaaagta atctataaca
     3061 agaagagaga aaacgcgaag aaacgaagtt tctgaaatct tcgtttcttc gcgttttgtt
     3121 ttaaattaca atgttaacga tgttaacatt gcagacgatg tagatatgtt tacaatataa
     3181 aacttgttta cacataaact tatggaaact tatacagtcg aaattttaga gccgaaagcg
     3241 aagaaattgc ttgacgattt ggcaaatcta aatttaatca aattacagaa aaccgaaaag
     3301 ccggagcaga aaaagcgtaa gttcggaagc atgaaaggtt tggtcaaaca tattgccgaa
     3361 gattttgacg ctccgctcga agattttaaa gactatatgt aatgcgattg ctaattgaca
     3421 ctcatatctt gatttggttt ctcgaaggct caaaacttct gtcaaattcg c
//
LOCUS       DASWNE010000572         3726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_477787, whole genome shotgun sequence.
ACCESSION   DASWNE010000572 DASWNE010000000
VERSION     DASWNE010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477787"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VGB68_11305"
     CDS             <1..511
                     /locus_tag="VGB68_11305"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEY0049868.1"
                     /translation="VPARINLAKLEAKLVGDFDAIFRLRDRIEEIRSQYDVIFFDTPP
                     TLGLITVNALVAATHVLIPIQSSYFALEGTDDLLETIEKVRSRPNPDLQLLGVLVTLF
                     DRRTSLSKDVEAHIRKVFGDKAFKTIITRSVRLEESPAHKETIFTYAPNSSGAGEYAK
                     LCKEVLKRG"
     gene            504..1379
                     /locus_tag="VGB68_11310"
     CDS             504..1379
                     /locus_tag="VGB68_11310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEY0049869.1"
                     /translation="MAKRGLPNGVGLRHDSHYVEDLARTNRSVGRLLPIDKIEPNPEQ
                     PRVEIGDLTELSSSIKEKGVLEPLLVKPNRQTGTWMIIAGERRWRASNLAGLREVPCI
                     ELDIDEKEVAEIALIENLQRKDLNVWEEADGLAALGSRFGYTHDEIARKIGKSRTTVT
                     EAMAIAGLPGDIRRKCIDARINAKSTLLEIARQFDENAMHQFLERIQNKNREEIRQAA
                     RPHLSSKAEPFTNSSQASAGKRSNTENTEKRKVFRYISEDKDFSIEIFSKNKSFGNAE
                     IKDALQQALNKLQEE"
     gene            2151..2717
                     /locus_tag="VGB68_11315"
     CDS             2151..2717
                     /locus_tag="VGB68_11315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0049870.1"
                     /translation="MSERQTSEITQILQAWNDGNEAAKEQLLPFVYDELRRQARRLMS
                     KERSNHTLQPTALVHEAFLRLSRQSGVEWRDSSHFYGIASRLMRQILVDHARLHATAK
                     RGQTPIHLSFEDLQVAIEDRAEAILVLNEVLERLEKFDEQQAKIVEMRFFGGMNNPEI
                     AEALDISERTVGREWQSARLWLYRELNK"
     gene            2793..>3726
                     /locus_tag="VGB68_11320"
     CDS             2793..>3726
                     /locus_tag="VGB68_11320"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0049871.1"
                     /translation="MQSENWKTIKEVLVEALKLDASERSDFLDKADISSEIRQEVESL
                     LTFEEESKDLMNLSAVEFSKDFFDADEEAKNILIGQRVGVYEIARELGYGGMGAVYLA
                     ERTDGKFKQKVALKLLKREMNTSALRQRFQQEREILASLQHPNIARLLDAGTTGDRIP
                     FIAMEYVEGLPIDAFCNKHDLDLTDRLKLFRTVCATVDFAHRNLIVHRDLKPSNILVT
                     EDGVPKLLDFGISKILSTDRENINAATITRLGVMTPSYASPEQLQNKSVTTATDVYSL
                     GVILYELLSGHRPFENKEGDLEEIYRAVIDGDPPP"
BASE COUNT         1041 a          934 c          890 g          861 t
ORIGIN      
        1 tgtcccggcg cggataaatc tcgcgaaact cgaagcgaag ctcgtcggcg atttcgacgc
       61 catttttcga ctccgcgacc gtatcgaaga aattcgctcg caatacgacg taatcttttt
      121 cgatacgccg ccgacgctcg gattaatcac agtcaatgcg ctggtcgccg cgacccatgt
      181 cttaattccc atccaatcat cctatttcgc actggaagga accgacgacc ttctggaaac
      241 catcgaaaag gttcgctcgc gcccgaaccc ggatttgcag cttttaggcg tcctggtcac
      301 gcttttcgac agaagaacgt cgctctccaa agacgtcgaa gcgcatatcc gaaaggtttt
      361 cggggataaa gcctttaaaa ccataattac gcggagcgtc cggctcgaag aatcgcccgc
      421 ccacaaagaa acgattttta catacgcgcc aaactccagc ggtgccggcg aatacgccaa
      481 gctttgtaag gaggtactga aacgtggcta agagaggtct gcccaacggt gtcggcttgc
      541 gacacgattc acactacgtc gaagatttag cccgcacgaa tcgttcggtc gggcgacttc
      601 tgccgattga caaaatcgag ccgaaccccg aacagccgcg cgtcgaaatc ggcgatctga
      661 ccgaattgtc gagttcaatc aaggagaaag gcgtcctcga accgcttctg gtaaaaccca
      721 atcgtcaaac cggaacctgg atgattatcg ccggcgagcg tcgctggcgc gcgtcgaatc
      781 tggcgggctt aagggaagtt ccttgtatcg aactggacat cgacgaaaaa gaagtcgccg
      841 agattgccct tatcgaaaac ctgcagagaa aggacttaaa cgtctgggaa gaagccgacg
      901 ggctcgccgc gctcggctct cgtttcggct acacgcacga cgaaatcgcc agaaaaatcg
      961 gcaaaagccg cacgaccgtc accgaagcga tggcgatcgc cgggctgccg ggcgatatcc
     1021 gtcggaaatg catcgacgcc agaattaacg ctaaatcgac gctgctggaa atagccagac
     1081 aattcgacga aaatgcgatg caccagtttc tcgaacgcat ccaaaacaaa aaccgtgagg
     1141 aaataagaca agcggcacgc ccgcatctct cgtcgaaagc cgagcctttc acaaattcca
     1201 gccaggcatc agccggtaaa cgctcgaata ccgaaaatac agagaaacga aaagtcttcc
     1261 ggtatatttc agaagacaag gatttctcga ttgaaatctt ttcaaaaaat aaatctttcg
     1321 gaaatgccga aatcaaagat gccttgcagc aagcccttaa taagctacag gaagaatagc
     1381 gcattttccg gcatataata cataatcttt attatcggac gcggtgaata tatcataact
     1441 aatcaaattt ttcgttaagc gtccgattaa ctaaatctcc ctacatccat aaactgaaag
     1501 cactgacatt cagaatatcc ttcgcttcgg ataaaggttc atttttttca tttacacaaa
     1561 tccatacttg tacacaaatg catacttgaa agcattcact tgaaattttc caaaataatt
     1621 ttcttaagga gactcaaata aggagactca aataaggaaa taaacaaatt ctttattgta
     1681 agaatgcatc gcccgttgca attatcgcta aagccgtcgc actgagcggc aaccggaaag
     1741 agacacgcta attcaacaga agaaagaagg tagttttaaa ttttttcagt ttcataaaaa
     1801 tagctcctaa atcctaattc cccgcttcgc cctgtaagct gtaataaatt ccccggtttt
     1861 tcgtaaataa tttgtttaag agttgcagat tgataaggtt atcgtcagcc ttatgcccga
     1921 caatgacact ggcagacgtt tcttttttca tttttcattt ataaacttcg gatttccttc
     1981 gtaatttcag gatttccggc agcgattccg aatcggctta aattttcagg ctgttttctt
     2041 aaatgccttt gcgggaaaat ccggaaaagt cggacaaagc tgctcgaaaa tttttatttt
     2101 gccggaaatt agtctacaat ttgtggtaac cacctgttta cacaacacat atgagcgagc
     2161 ggcaaacttc ggaaatcact caaattctgc aagcctggaa tgacggaaac gaagcggcaa
     2221 aagaacagct tttgccgttt gtttacgacg agctcagacg acaggcgcgg cggctgatgt
     2281 cgaaagagcg ctcgaatcat actttgcagc cgacggcgct ggtgcacgag gcttttttgc
     2341 gtctcagccg gcagagcggc gtcgagtggc gcgacagcag tcatttttac ggtatcgctt
     2401 cgcgcctgat gcgccagatt ctggtcgacc acgcccgtct gcacgcgacg gcaaagcgcg
     2461 ggcagactcc gattcattta tcgttcgaag atttgcaggt ggcgatagaa gaccgcgccg
     2521 aagcgattct cgttttgaac gaagttctgg aacggctgga gaaatttgac gagcagcagg
     2581 cgaaaatcgt cgagatgcgg tttttcggcg ggatgaataa cccggaaatc gccgaagccc
     2641 tggacatttc cgaaagaacc gtcgggcgcg aatggcagtc ggcgcgactc tggctttacc
     2701 gcgaactgaa taaataaaat caaattccag atttgttgtc cgcttttccg caaaaaatcc
     2761 gcatatctgt aaagcatacg ggaaaattta ttatgcagtc tgaaaactgg aaaactatca
     2821 aagaagtttt ggtggaagct ctcaagctcg atgcttccga gcgaagcgat tttctggaca
     2881 aagccgacat cagctccgag attcgacaag aagtcgaatc gctcctgact tttgaagaag
     2941 agtccaaaga tttgatgaat ttatcggcgg tcgagttttc gaaggatttt tttgacgccg
     3001 atgaagaagc gaaaaatatt ttaatcggtc agcgcgtcgg cgtttatgaa atcgcgcgcg
     3061 agctcggcta cggcggcatg ggcgcggttt atctcgccga gcgaaccgac ggaaaattta
     3121 aacaaaaagt ggcgctgaaa ctgctcaagc gcgagatgaa cacgagcgct ttgcgccagc
     3181 ggtttcaaca ggagcgcgag attctcgcct cgctgcagca tccgaacatc gcccgcctgc
     3241 tcgacgccgg aacgaccggc gaccggattc ccttcatcgc tatggaatac gtcgaaggct
     3301 tgccgattga cgcgttctgc aacaaacacg atttggattt gacggacaga ttaaaattat
     3361 ttcgcacggt ctgcgcgacg gtcgatttcg ctcatcgaaa tttgatcgtc caccgcgatt
     3421 tgaagccctc gaatatcctc gtcaccgaag acggcgtgcc gaagctgctc gatttcggca
     3481 tctcaaagat tctttcgacc gacagagaaa atataaacgc ggcgaccatt accaggctcg
     3541 gcgtgatgac gccttcttac gcctcgcccg aacaattaca gaataaaagc gtgacgaccg
     3601 ccaccgacgt ttacagcctg ggcgtgattt tatatgagct tttaagcgga catcgaccgt
     3661 ttgaaaacaa ggaaggcgac ttggaggaaa tttatcgagc cgttatcgac ggcgacccgc
     3721 cgccgc
//
LOCUS       DASWNE010000573         3821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_196313, whole genome shotgun sequence.
ACCESSION   DASWNE010000573 DASWNE010000000
VERSION     DASWNE010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196313"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="VGB68_11325"
     CDS             <1..750
                     /locus_tag="VGB68_11325"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY0049872.1"
                     /translation="GGKNMSKINRRNFLSAFGAMSAGLALSQNSNAQTRTGNVNILTD
                     EEKAQKLIQASRQRLRDLYFPNVELLTHEGKKVRFYDDLIKDKIVTINFMYATCDGIC
                     STISRNLALAQEMLGDMVGKDVFMYSITLKPVQDTVEVLNAHARAMKAKPGWLFLTGK
                     PEDIELIRRKQGFVDLDPKVDKDKSQHTGNVRYGNEPMQYWGSCPGLGDPEVIVESLI
                     WVDESLKEKYLARKERKKGGYGQGTKKGGGK"
     gene            747..1709
                     /locus_tag="VGB68_11330"
     CDS             747..1709
                     /locus_tag="VGB68_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049873.1"
                     /translation="MKSMILKTALFALLLTVEICSMSIRQVFSQKAVASSEQTTNYTV
                     GDVGAEFAVFDGAYVWIANQFSDTVTKINGSTGVIAGNYEITGNPLGIAFDGRYIWVS
                     RYASDDVVKMDVNNGAILAAVKVNNGPGFLLAKDGHIWVVNRIANTVQKIPVDGTTVS
                     ATYKVGKKPAMMETDGANIWVANSQSHSVTRINIASGETATFASGGKGAFGIAFDGTN
                     IWVSNFFSGNIVKFSGSGNLIGNYKIGDGVAGMYYAQGYMWVVANGESLVYRVRLSDG
                     SIVSSHTVSPSPYGLLYDGRKFWIPSSGSGKISVVTPLPADVTN"
     gene            complement(1711..1911)
                     /locus_tag="VGB68_11335"
     CDS             complement(1711..1911)
                     /locus_tag="VGB68_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049874.1"
                     /translation="MKKASLSSERSLFYKSQPILSDKSIRRRNNGAGSGESAIRVFYC
                     QIPPMMPKTRVIAIEEALFAYA"
     gene            2176..3408
                     /locus_tag="VGB68_11340"
     CDS             2176..3408
                     /locus_tag="VGB68_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049875.1"
                     /translation="MKKQIILTTLILNFVMAVSAFAQDAPVSELTAEKISTNVVRLSG
                     AENAVYSRTGKFLAIQMASEFLLLPARKPFESFAELCSARRHSGRIIGFLSTDTLVYA
                     AGGEIFALDAAGQRSRKLFSQNSGQKLADKVLSQREFVFVSDDLIIAGNGSYDMGGNP
                     GNIYRFDLKNRRVKKGAPIKQFWEAALSPGGKYILYERGGEQSNYIDFYDVRLDKNYE
                     AERRFNFRKAFPRFSEWSASPLVWVGSRDAFLAYVTSGVHPDEIKPEDAEEHNKTWLC
                     LLDAPTGRIIWKKLIKTGAFQSGFQPLNSDKVLVNYDEEVYELSLADGKERRIRGIGG
                     KSLVISPDKKKIAFIKSNRAFTALLNGENKKPVLDLPRGWKPATDYKGMGERPPVWSP
                     SGDGLILFGENQLLIARL"
     gene            3578..>3821
                     /locus_tag="VGB68_11345"
     CDS             3578..>3821
                     /locus_tag="VGB68_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049876.1"
                     /translation="MTDEFLAFTTETLATYGFRGEAFYLFFSGTLQILLANQSMLNDI
                     FGYFILILVIFLSVILPLMFIVPTILNWRKNRRLMRD"
BASE COUNT         1081 a          822 c          952 g          966 t
ORIGIN      
        1 ggaggaaaaa atatgtcaaa gataaacaga agaaactttc tctcggcatt cggtgcgatg
       61 tcggcaggtc ttgccctgag tcaaaattcc aatgctcaaa cccgaaccgg caacgtaaat
      121 atactgaccg atgaagaaaa agcccaaaaa ctaatccagg cttcccggca aaggctgcgc
      181 gatttgtatt ttcccaatgt cgagctactt actcacgagg ggaaaaaagt gagattttac
      241 gatgatttaa taaaagataa aatcgtgacc atcaacttta tgtacgccac ctgcgacggg
      301 atttgttcaa ccatcagccg caatctggca ttagcacagg aaatgctcgg cgacatggtc
      361 ggcaaagatg tttttatgta ttccattact ttaaagcccg tgcaggacac ggtcgaagtt
      421 ttgaatgcac acgcccgggc gatgaaggcg aagccgggct ggttatttct gaccggaaaa
      481 ccggaagata ttgaattaat caggcgcaaa caaggatttg tagatcttga tccgaaagta
      541 gacaaggata aaagtcaaca tacgggaaat gtccggtatg gcaatgagcc gatgcagtat
      601 tgggggtctt gtccgggctt gggcgatcct gaagtaatag tcgaatcgct gatttgggta
      661 gacgagtcgc ttaaagagaa gtatttagcc cggaaagaaa gaaaaaaagg cggatacgga
      721 caaggcacga aaaaaggagg cggaaaatga aaagcatgat tttaaaaacg gcgctcttcg
      781 cgcttctgtt aacggtagaa atctgctcga tgtcgatcag gcaggtattt tctcaaaaag
      841 cggtggcgag cagcgagcaa actactaatt atactgtcgg cgacgtgggc gcggagttcg
      901 cggtttttga cggagcatat gtttggatag ccaatcaatt cagcgatacc gtcacaaaaa
      961 taaacggctc gaccggcgtt attgcaggta attatgaaat caccggaaat ccgctgggaa
     1021 tagcctttga cgggagatat atctgggtaa gtcgttacgc ctcggacgac gtggtgaaga
     1081 tggatgtcaa taacggagcc attctcgctg ccgtcaaagt taataacgga cccggatttc
     1141 ttctggctaa ggatggtcat atctgggtag taaacagaat tgcgaatacg gtgcaaaaaa
     1201 ttccggtaga cgggacgacg gtttcagcca cctacaaagt cgggaagaaa cccgctatga
     1261 tggaaactga cggagccaat atctgggtgg caaacagtca aagccattca gtaactcgaa
     1321 tcaatatagc cagcggcgaa accgcgacat tcgcatccgg cggaaaaggc gctttcggaa
     1381 ttgctttcga cggaaccaat atctgggtaa gtaatttttt tagcggcaac atcgtcaaat
     1441 tctccggcag cggaaacctt atcggaaact acaaaatagg cgacggagtt gccggaatgt
     1501 attatgcaca gggatatatg tgggttgtcg ccaatggaga gagcctggtt taccgggtca
     1561 gactttctga cggctcgatt gtcagcagtc atacggtttc cccgtctcca tacggattac
     1621 tttacgacgg gcggaaattt tggataccat cttccggcag cggaaaaatt tccgtagtaa
     1681 ctccgctgcc tgccgacgtt actaattaat ttaagcataa gcaaataaag cttcttcgat
     1741 agcaataaca cgggtcttag gcatcatcgg cggaatttgg caataaaaaa ccctgattgc
     1801 cgattctcct gaaccggcac cgttattccg acgccggatg cttttgtcag ataaaattgg
     1861 ttgagactta taaaaaaggc ttcgctctga actgagcgaa gcctttttca tttggcgttt
     1921 atagtaaatt tcgatgtttt ttaaaatttc ggaatttaaa gaaatctcaa acccatatca
     1981 ccacacattt gtgtagacaa ctttccattt gtcagagaaa agccgcggca ttttaataaa
     2041 atgtcgtggc ttttgcgcag tttttcgaag aaaatgttga taaaaacgtc ggatttctac
     2101 acatttgtgc ggtgcgcccg aaaatctctc tgagctaaat tctttgtagt aattatttcg
     2161 aggagaaagc tttgaatgaa aaaacaaatt attttaacga cacttatttt gaattttgta
     2221 atggctgtga gcgccttcgc gcaggacgct ccggtttcgg aattgaccgc cgagaagatt
     2281 tccacgaatg tcgtgcggct ttccggcgcg gaaaatgcgg tttattcccg cacgggaaaa
     2341 tttctggcga ttcagatggc gtcggaattt ctgcttctgc ctgctcgaaa gccgttcgag
     2401 tcatttgccg aactctgctc ggctcgccgg cacagcgggc gcattatcgg ttttctttcg
     2461 acggatacgc ttgtctatgc cgccggcggg gaaattttcg cgctcgacgc cgccggtcaa
     2521 agaagccgta aattattttc tcagaacagc gggcaaaagc tcgccgataa ggttctctcg
     2581 cagcgtgagt tcgtgtttgt ttccgacgat ttaatcatcg ccggcaacgg cagttacgat
     2641 atgggcggaa atcccggaaa tatttacagg tttgatttaa aaaaccgccg cgtgaagaaa
     2701 ggcgcgccga ttaaacagtt ctgggaagcc gctctatctc cgggcggaaa atatattctc
     2761 tacgagcgcg gcggcgaaca aagcaattac attgattttt acgacgtccg gctggacaaa
     2821 aactacgaag ccgaaaggcg tttcaatttc agaaaagcgt ttccgcgatt tagcgaatgg
     2881 tctgccagcc cgctggtctg ggtcggcagt cgcgacgcat ttctcgctta cgtcacttcg
     2941 ggagtccacc cggacgagat aaaaccagag gacgcagaag aacataataa aacctggctt
     3001 tgtttgctgg acgcgccgac gggacgaatc atctggaaaa aattaattaa aacgggcgcg
     3061 ttccaaagcg gattccagcc gctaaacagc gataaagtgt tggtgaatta cgatgaagaa
     3121 gtttacgaac tgtcgctcgc cgacggcaaa gaacgtcgga ttcgcggcat cggcgggaaa
     3181 tctctggtca tcagtcccga caaaaagaaa atagctttta ttaaatccaa tcgcgctttt
     3241 acggctttgt taaacggcga aaacaaaaag ccggttctcg atttgccgcg cgggtggaag
     3301 cccgcgaccg attacaaggg tatgggcgaa cgtccgcccg tttggtcgcc gtcgggcgac
     3361 gggctgattt tattcggcga aaatcaatta ctgatagcgc ggctctaaag cgaaacattc
     3421 gaaatcaaaa aaaattaaaa tcgcggagat aagccgcaaa atcctgctct tgggaataat
     3481 tctacacatt tgtgtagtgc gcgcgattta tttcgcttgt tatactgacc ggcgaaatcg
     3541 aacttattca atacgaattt aaaggagaat tagagtaatg acggacgaat ttttagcttt
     3601 tacaacagaa acgctggcga cttacggctt tcgcggcgaa gcgttttatt tatttttttc
     3661 gggaacgctt caaattctgc tggctaatca gagtatgtta aacgacattt ttggttattt
     3721 tatcttaata ctcgtcattt ttctgtctgt tattttgccg ctgatgttca tcgttccgac
     3781 gattcttaac tggcgaaaaa acagacgttt aatgcgagac g
//
LOCUS       DASWNE010000574         1950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_615622, whole genome shotgun sequence.
ACCESSION   DASWNE010000574 DASWNE010000000
VERSION     DASWNE010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615622"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(288..>1950)
                     /locus_tag="VGB68_11350"
     CDS             complement(288..>1950)
                     /locus_tag="VGB68_11350"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEY0049877.1"
                     /translation="LSLVVAGTKYRGQFEERLKTILKELKENDQYIIFIDELHTLVGA
                     GSAEGSLDAANILKPALSRGEIQSIGATTPAEFRKSIGKDRALERRFQAVKILPPNEA
                     EALKIVQGLAERYETFHQIRYSKEALETAVYQSNRYITDRFLPDKAIDVLDEAGARAK
                     LKFQNEMGEPSWKETVANWKRATANDDKLIALELQSLEESFIAVEVSKEDVEEVIARW
                     TGIPVQSIKQEEAQKLLKIEAELHRRVISQRPAISALARAIRRSRAGFKNPNKPVGSF
                     LFLGATGVGKTEVARSLAEFLFGAERALVRFDMSEFMERHTVAKFIGSPPGYVGHEEG
                     GQLTEKLRRQPYSVVLFDEIEKAHPDIFNVLLQVFEDGILTDSQGNTIDCKNAIFIMT
                     SNIGARFIQKRASLGFQASSDASREKMEEQVMSAVKQTFAPEFINRLDEIVIFDELSD
                     KDLLQIIDLQVAKLNEILEKRTLQIRLTDEAKSWLIVKTCADRKFGARPLKRALQKYV
                     EDELSEALIQGTLTDDNLVEIYCENEKLNYRTISIGEIEDAVLAG"
BASE COUNT          440 a          517 c          470 g          523 t
ORIGIN      
        1 cggttccctt ttattattgc aatattttag gcttcgataa gcctctgttt ataaaaaaaa
       61 cgctcgagat tcaaacttaa aattgtatcg ttaaaattag ctatttcaaa gctgacgatt
      121 cgcgttgggt ttatacgcga agtttgattc gctcctctag ggctaggttg cctatccgga
      181 tttacccaac aaattccgaa attatacagg taaatactga aaaaaagaaa agcacgcgag
      241 agttttcggc aaactcttgc gtgcctttta acgaatttta caaatcttta cccggctaaa
      301 accgcgtctt cgatctcacc gatgctgatc gtccggtaat ttaatttctc gttttcgcaa
      361 tagatttcga ccagattgtc gtccgtcaga gtgccctgaa tcagggcttc ggaaagctcg
      421 tcctcgacat atttttgcag cgcgcgtttc agcgggcggg cgccgaattt gcggtcggcg
      481 caggttttta caatcaacca gctcttagcc tcgtcggtca gacgaatctg caacgtgcgt
      541 ttttccagaa tctcgttcag tttcgcgacc tgtaaatcga tgatttgcag taaatcttta
      601 tccgaaagct cgtcgaagat aacgatttcg tccagacgat tgataaattc cggcgcgaaa
      661 gtctgtttga cggcgctcat cacctgctct tccattttct cgcgcgaagc gtccgagctt
      721 gcctgaaatc cgagcgacgc gcgtttttga ataaatctcg cgccgatatt cgaagtcatt
      781 ataaaaatcg cgtttttgca gtcaatcgtg ttcccttggg aatcggtcag gatgccgtct
      841 tcaaaaacct gcaaaagcac gttgaaaata tccgggtgcg ctttttcgat ttcatcaaac
      901 aaaacgaccg aatacggctg gcggcgaagc ttttccgtca actgcccgcc ttcttcgtgc
      961 ccgacgtatc ccggagggct tccgataaat ttcgccaccg tgtgccgctc cataaattcc
     1021 gacatatcga agcgcacgag cgcgcgttcc gcgccgaaaa gaaactccgc caggctgcgc
     1081 gcgacttccg ttttgccgac gccggttgcg cctaaaaata aaaacgagcc gaccggcttg
     1141 ttcggatttt taaagcccgc ccgcgagcgc cgaatcgcgc gcgccagagc ggaaatcgcc
     1201 ggacgctgcg aaataacgcg ccggtgaagc tcagcttcta tttttaaaag cttctgcgct
     1261 tcttcctgtt tgattgattg gacgggaatg cctgtccagc gcgcgataac ttcttcgacg
     1321 tcttctttcg agacttcgac ggcgataaaa gattcttcca gactttgcag ttcaagggcg
     1381 attaatttat cgtcgttcgc ggtggcgcgt ttccagttgg cgacggtttc cttccacgaa
     1441 ggctcgccca tctcgttttg aaatttcagc ttggcgcgcg cgcccgcttc gtccagaacg
     1501 tcaatcgcct tgtccggcaa aaagcggtcg gtgatgtacc ggttcgattg ataaaccgcc
     1561 gtctccagcg cttctttcga atagcgaatc tggtgaaacg tctcgtaacg ctccgccagt
     1621 ccctggacga ttttcaaggc ttcggcttcg ttcggcggga gaattttaac ggcttgaaag
     1681 cggcgttcga gcgcgcggtc tttgccgatt gatttgcgaa actcggcggg cgtcgtcgcg
     1741 ccgatagatt gaatctcgcc gcgcgagagc gccggtttta gaatatttgc cgcatccagc
     1801 gagccttccg ccgagcccgc gccgacgagc gtatgcagct cgtcgataaa aataatatat
     1861 tggtcgtttt ccttgagttc cttcaaaatc gtcttgagcc gttcttcaaa ctgcccgcga
     1921 tatttcgtgc cggcgacgac caaagataaa
//
LOCUS       DASWNE010000575         2577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_124114, whole genome shotgun sequence.
ACCESSION   DASWNE010000575 DASWNE010000000
VERSION     DASWNE010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124114"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /gene="cdaA"
                     /locus_tag="VGB68_11355"
     CDS             <1..842
                     /gene="cdaA"
                     /locus_tag="VGB68_11355"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HEY0049878.1"
                     /translation="TPVSEYLPAFNTVRNAIDIVLVFIIVYVLLKLLRGTRGMPMAVG
                     MVTLGLLYWLAVAQDLATLEFVLRGALLYVGVAVIVLFQSEIRQALIYFGNQLRFPVF
                     RRQRGQLGETIYDEVVLAATTLASEKIGALIVLERNVGLRNLSDAGVQLDAKLTYDLL
                     VTIFNPSTPLHDGAVVIQRERITAASVFLPLTKNPSISRELGTRHRAAIGITEDTDAV
                     SIVVSEETGLISFIDAGRMKRNLDTNQLRSLLLNAMDIPVIERKRKATKTMKEADSEI
                     TIG"
     gene            926..1864
                     /locus_tag="VGB68_11360"
     CDS             926..1864
                     /locus_tag="VGB68_11360"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HEY0049879.1"
                     /translation="MLFSFPTEKFFEKEQRQMFFHRLIRRVFLEDWGPKLVALGITIA
                     LWLGVTGLQKPTSRRLNSVPLVLNFSSDMEATNSPLKEVDIVVTGDKTKISRLRSEDL
                     VVSIDLTDVTAGERTIQLTPENINVELPSGLKIDEIQPGKIAVKLERVEEREVAVKPE
                     IEGNTADGFEIYAQAVVPAKVRVRGPESFVNSLDSISTEKITLDNHKQDFIQRQVGLN
                     IVNPKVTLLDTIVDVTFRIGEKRTERLFVVPYKTETETKNATVLLFGARSILEELSAD
                     ELQIVFAPNENGENIPQIALPPEIQGKVEVKNFKIK"
     gene            2074..>2577
                     /locus_tag="VGB68_11365"
     CDS             2074..>2577
                     /locus_tag="VGB68_11365"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY0049880.1"
                     /translation="MKKKLLISFALFVWFSQSALAQLSTAVLVQIVRAEDERRYDKTL
                     ENLLLSPDAQIRARAALAVGRIGDERSIPALADLLDKDSDTVREMAAFAVGEIESAKG
                     ADIILKTLENEKNSAAVRARSLEAAGKIGAAIATADKKKSDELGEAILEAIEFERSQP
                     KPDKQLIL"
BASE COUNT          644 a          656 c          673 g          604 t
ORIGIN      
        1 agacgcctgt tagcgaatat cttccggcat ttaacaccgt gcgaaacgcg atagacattg
       61 tgctggtttt cataattgtc tatgtcctgc tcaagctttt gcgcggcact cgcggtatgc
      121 cgatggcggt cggtatggtc acgctcggtc tgctctactg gctggcggtc gctcaggatt
      181 tggcgacgct cgaattcgtt ttgcgcggcg cgctgcttta tgtcggcgtc gccgtcatcg
      241 tgctttttca atcggaaatc cggcaggctt tgatttattt cggcaaccaa ctgcgctttc
      301 ccgtttttcg ccgccagcgc gggcaattgg gcgaaacgat ttacgatgaa gtcgtgctgg
      361 cggcgaccac gctggcgtcg gagaaaatcg gcgcgctgat tgttctggaa agaaatgtcg
      421 gtttgcgaaa tctgagcgac gccggcgttc agctcgacgc gaaattgact tacgatttgc
      481 tggtgacgat ttttaatccg tccacgccgc tgcacgacgg cgcggtcgtt attcagcgcg
      541 agcgcatcac cgccgcttcg gtctttctgc cgctgacgaa aaatccgagc atctcgcgcg
      601 agctcggcac gcgccatcgc gccgccatcg gcattaccga agacaccgac gccgtttcga
      661 tcgtcgtctc cgaagaaacc ggcttgattt cttttatcga cgccgggcgg atgaagcgaa
      721 atctggacac caaccagctt cgcagcttgc ttttaaacgc gatggacatt cccgtcatcg
      781 aaagaaaacg aaaggcgacc aaaacgatga aggaagccga ttcggaaatc acgataggat
      841 aacgcaaaat gcaaagcgca gaatgcaaaa tgtaaatatc gggctttaat ttttcattat
      901 ccgttttcag ttttacgttg tatttatgct tttctcgttc ccgaccgaaa aatttttcga
      961 aaaagagcag cggcaaatgt tttttcaccg cttgattcgt cgcgtctttc tcgaagactg
     1021 gggaccgaag ctggtcgcgc tcggcatcac gattgcgctc tggctcggcg tgaccggact
     1081 gcaaaaaccg acttcgcgcc ggctgaacag cgtcccgctg gttctgaatt tttcgagcga
     1141 tatggaagcg accaactcgc ctttgaaaga agtcgatatt gtcgtcaccg gcgacaaaac
     1201 gaaaatttcc cgcctgcgaa gcgaagattt ggttgtttcg attgatttga ctgacgttac
     1261 ggcgggcgag cgaaccattc agctgacgcc ggaaaacata aacgtcgagc tgccgagcgg
     1321 cttgaaaatc gacgagattc agccgggcaa aatcgccgtc aagctggaaa gggtcgaaga
     1381 acgcgaagtt gccgtcaaac cggaaatcga aggaaatacc gccgacggtt tcgagattta
     1441 cgcgcaggcg gtcgtgccgg caaaagtgcg cgtgcgcgga ccggaaagct tcgtcaattc
     1501 gctcgattcg atttccaccg aaaaaatcac gctggataat cacaaacagg attttatcca
     1561 gcgacaggtc ggcttaaaca ttgtcaatcc taaagttacg ctgctcgata cgatagtcga
     1621 cgtcacgttc agaatcggcg aaaaacgcac cgaaagattg tttgtggtgc cttataaaac
     1681 ggaaaccgaa acgaaaaatg cgaccgttct gcttttcggc gcgcgctcga ttctggaaga
     1741 gctcagcgcc gacgagctgc aaatagtatt tgcgccgaac gaaaacggcg aaaacattcc
     1801 gcagattgct ctgccgcccg aaatacaggg caaagtcgaa gtcaaaaatt ttaagataaa
     1861 ataataaaga aattagccgc tgagagagag agaaattgaa acaacgggtt aacgcggatt
     1921 aacgcggatt tatttttcct ggtaatccgt gtcttttggc gtcaatccgt tgttttaaat
     1981 cttttcttta tattctccct gtgtcttctg tggctaattt tctttgtgtt gtaaaataac
     2041 tctcgatatt ccgaaagagc ttgtaattgg aaaatgaaaa aaaaattatt aatttctttt
     2101 gccctttttg tatggttttc gcaaagcgcg ctcgcccagc tttcgaccgc cgtgctagtg
     2161 caaatcgtcc gcgccgaaga cgagcgccgc tatgataaaa cgctcgaaaa tcttttgctg
     2221 agcccggacg cgcagattcg cgcccgcgcc gcgctcgccg tcggcagaat cggcgacgaa
     2281 cgctcgattc ccgcgctcgc cgatcttctg gacaaagatt cggatacggt tcgcgaaatg
     2341 gcggcgttcg ccgtcggcga aatcgaatcg gcaaaaggcg ccgacatcat tttgaaaacg
     2401 ctcgaaaacg aaaaaaattc cgccgccgtg cgcgcccgga gcctcgaagc cgccggaaaa
     2461 atcggcgcgg caatcgcgac ggctgataaa aagaaatccg atgagctggg cgaagcgatt
     2521 ctcgaagcca tcgagtttga acggagccag ccgaaaccgg acaaacaatt gattctg
//
LOCUS       DASWNE010000576         1822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_267368, whole genome shotgun sequence.
ACCESSION   DASWNE010000576 DASWNE010000000
VERSION     DASWNE010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267368"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VGB68_11370"
     CDS             complement(<1..462)
                     /locus_tag="VGB68_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049881.1"
                     /translation="MKNLLLIFITAAAFGTTACLPLNFAQSSRAVNSNAANENAALTA
                     ENAAPENKTEISATPKPAPKKLSNAVCPDPLKPCHHREREFADWELPFNLPARVVANK
                     NYSSASFYAVILKKYDDACADEMDYDSEIEAERIAAQKAFPKRKVFAEYSCP"
     gene            561..>1822
                     /locus_tag="VGB68_11375"
     CDS             561..>1822
                     /locus_tag="VGB68_11375"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049882.1"
                     /translation="MLNSLTYFLPADLENAVQTKFDEWKTENRIARIWSKDAGVWTND
                     DESKWLGWLDSIETELKDLQKYRDFAADAAEFSDVVVLGMGGSSLAPEVLSITFGKRN
                     FHVLDSTVPAQIKALESRLDLAKTLFIVASKSGSTLEPNTFKQYFFERVSQTVGRENA
                     GRQFAAITDPNSKMQQVAERDGFRKIFFGDPQIGGRFSALSPFGLAAGAAMGLDFEDF
                     LRRASEMASACKNENPGENPGAILGTILGVCHNEGRDKLTIFTSPEIYDVGAWLEQLI
                     AESTGKQGVSIIPIDREPLLPAEDYGDDRVFAYLKLKDSSDDSPDEKVSALEAKGHPV
                     VKIEFDDKSNLGQEFFRWEFATAVAGALMKINPFNQPDVESAKIEAKKITEEYEEKGE
                     LPAETPFYEAEGIKLFTSEKYSGELRQFV"
BASE COUNT          444 a          427 c          521 g          430 t
ORIGIN      
        1 cgggcagctg tattcggcaa aaactttgcg ttttgggaaa gctttttggg cggcgatgcg
       61 ttcggcttcg atttcggaat cgtagtccat ttcgtcggcg caggcgtcgt cgtatttttt
      121 caaaatgacg gcgtaaaacg acgcggacga atagttttta ttggcgacga cgcgggcggg
      181 cagattaaac ggcagctccc agtcggcaaa ttcgcgttcg cggtgatgac acggttttaa
      241 agggtccggg caaacggcgt tcgacaattt tttcggcgcg ggtttcggcg tcgccgaaat
      301 ttcggttttg ttttcgggcg cggcgttttc cgccgtcaaa gccgcgtttt cattcgccgc
      361 gttcgagttt acggcgcggc tgctctgcgc gaaattcagc ggcaaacagg ctgtcgtgcc
      421 gaatgcggca gcggttataa aaatcagtaa taagtttttc atataaattc tccggcttaa
      481 aaaaaatctc taattgataa cgcgaatttt tatcgcaaaa agagtcagca aaaccgattt
      541 tttttgctaa tctgaaagat atgctgaatt cattaactta ttttttgccg gcggatttgg
      601 aaaacgccgt tcaaacgaaa ttcgacgaat ggaaaacgga aaacaggatt gcgcgtatct
      661 ggtcgaaaga cgccggcgtc tggacgaacg acgacgaatc gaaatggctc ggctggctcg
      721 attcgatcga aaccgaattg aaggatttgc agaaataccg cgattttgcc gccgatgcgg
      781 cggagttttc cgacgtcgtc gtgctcggaa tgggcggctc gtcgctcgct ccggaagttt
      841 tgtcgattac tttcggaaaa cgtaattttc acgttctgga ttcgaccgtt ccggcgcaga
      901 taaaggcgct ggaaagcagg ctcgatttag cgaaaacgct ttttatcgtc gccagcaaat
      961 ccggctcgac gctggagccg aacactttca agcaatattt cttcgagcgc gtttcgcaaa
     1021 cggtgggacg cgaaaacgcg ggcagacagt ttgcggcgat taccgacccg aactcgaaga
     1081 tgcagcaggt cgccgagcgc gacggctttc ggaaaatctt tttcggcgac ccgcaaatcg
     1141 gcgggcgttt ttccgcgctt tcgccgttcg gcttggcggc gggcgcggcg atgggcttgg
     1201 attttgaaga ttttctcagg cgcgcgagcg agatggcgtc ggcgtgtaaa aacgaaaatc
     1261 cgggcgaaaa tccgggcgcg attctgggaa ctatcctggg cgtctgtcat aacgaagggc
     1321 gcgataaatt gacgattttc acctcgccgg aaatttacga tgtcggcgcc tggctcgaac
     1381 aattaatcgc cgaatcgacc ggcaaacaag gcgtttcgat aattccgatc gaccgcgagc
     1441 cgcttctgcc agccgaagat tacggcgacg accgcgtttt cgcttatctg aaattaaagg
     1501 attcgagcga cgactctccg gatgaaaaag tttcggcgct cgaagccaaa gggcatccgg
     1561 tcgtgaaaat cgaatttgac gataaatcga atctcgggca ggaatttttt cgatgggaat
     1621 tcgcgacggc ggtcgccgga gcgttgatga aaatcaaccc gtttaatcag cctgacgtcg
     1681 aatcggcgaa aatcgaagcg aaaaaaatca cggaagaata tgaagaaaag ggcgagctgc
     1741 cggcggagac gccgttttat gaagcggaag gaatcaagct tttcacgagc gaaaaatact
     1801 cgggcgagct gcgccaattc gt
//
LOCUS       DASWNE010000577         3803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_89316, whole genome shotgun sequence.
ACCESSION   DASWNE010000577 DASWNE010000000
VERSION     DASWNE010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89316"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VGB68_11380"
     CDS             complement(<1..238)
                     /locus_tag="VGB68_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049883.1"
                     /translation="MNGYFYILLAALIIFIAFMIFQAVGGKLIGGKIEDLGVFVGPTI
                     FQFKLGEVTVRLNALPLGSYVKFSDDFQLLHPLRK"
     gene            complement(249..1676)
                     /locus_tag="VGB68_11385"
     CDS             complement(249..1676)
                     /locus_tag="VGB68_11385"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HEY0049884.1"
                     /translation="MRVAVLSAEAVPYSKTGGLGDVAGALPKALRQVGVDSVLITPCY
                     RQTKGEYLWRTAIDDLWVDWRGRSLRARAFYSEANGSPTFLIDAPEYFHRSSIYGFNE
                     DYERFAFFNHAALILLRRIGAAPDIVHLNDWHTGFAAVEIASKRFRDSYWRNTRTVLS
                     IHNMAYQGVFGAGELWKLGFGSDFERNAFLSDGAASALKAGLSAADMLSTVSRRYAEE
                     IQTAENGYGLEWLLRQRAGRLIGITNGVDYDVWNPETDPELPAHYSARDLSGKRECKR
                     AMLERFSLPVDLDKPVFANISRLTAQKGFELIQQVAGDIIASGAYFVALGSGEKSYED
                     FLQWLRDRAPRSVGIYKGYNESLAHVIEAGADVFLMPSRFEPCGLNQMYSLRYGTVPI
                     VRAVGGLDDTVENFDLVNGKGNGFKFNEFRADRFLEKIYESMFAYADGDAWRKLQWNG
                     MNEDNSWENAARKYVQLYDLTRQVG"
     gene            complement(1802..2905)
                     /locus_tag="VGB68_11390"
     CDS             complement(1802..2905)
                     /locus_tag="VGB68_11390"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY0049885.1"
                     /translation="MNNSQDKQKIETPVDGGWLTRERALVLVLIAATAIAFYVCYLIA
                     QPFIPAVVWATALAIAAYPLHGWILSRINRPNIAAGITVLIITLAIIVPGVFVGQTLI
                     SQIGKSAETLKKQTAPEQWQDAVQGNEKLAPLIKWVEPHFDIRSAAGSAAEAASSHVG
                     TVVGGLIWTIAQLLITFFTLFYFFRDKNLIVKTLKSLTPLSQSEVDKMFSRVGKIIYA
                     TLYGTIAVSMVQGFLGGLMFWWLGIPAPVLWGVVMFLLSLVPVLGAPAVWIPAAVYLL
                     LTGEPGKALLLAGWGALVIGAVDNLLYPVLVGDKVRMHPLLVFFSVIGGLALIGSAGL
                     VLGPVVVAVSGMLAEIWRQRTQEGNTAEEGVSG"
     gene            complement(3098..3730)
                     /locus_tag="VGB68_11395"
     CDS             complement(3098..3730)
                     /locus_tag="VGB68_11395"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEY0049886.1"
                     /translation="MSNLKVTEYAQHEKGLILYESLLKLEEKGIKKHYFPDIDAEVDV
                     TEMLKGFESPNKRLRRAMIKSPFAFTGREDLLIQYDVALSFAGEDRSYVSKVAQELKD
                     NNIEVFYDEYEQVNLWGKNLYDELDDIYRNRSEYVVMFISEHYAKKMWTDHERQSAFD
                     NAIQAKKEYVLPARFDNTELKGLRKTIGYISLENMTPKEFAQMIIKKLER"
BASE COUNT          974 a         1093 c          849 g          887 t
ORIGIN      
        1 tttttcgcag cgggtgcagc aattgaaaat cgtcgctgaa tttcacataa gaaccgagcg
       61 gaagcgcgtt cagcctgacc gtgacctcac ccagtttaaa ttgaaaaatt gtcggaccga
      121 cgaaaactcc caagtcttcg atttttccgc cgattaattt tccgccgacg gcttgaaaaa
      181 tcataaaggc gataaaaatt atcaaggctg ccagtaaaat gtaaaaataa ccgttcattt
      241 ttaggaattt aaccgacctg ccgcgttaaa tcgtataact ggacatattt gcgcgccgcg
      301 ttttcccacg aattatcttc gttcatcccg ttccattgca gcttgcgcca ggcgtcgccg
      361 tcggcgtagg caaacattga ttcgtaaatt ttttccaaaa accggtcggc gcgaaattcg
      421 ttaaacttaa agccgttgcc cttgccgttg accaaatcga aattctcgac cgtatcgtcc
      481 agaccgccga ccgcgcgcac aatcgggacg gttccgtatc tcaaagaata catctggttc
      541 aagccgcacg gctcaaagcg cgaaggcatc aaaaacacgt ccgcgcccgc ttcaatgacg
      601 tgcgccagcg attcgttgta gcctttataa atgccgacgg agcgcggcgc gcggtcacgc
      661 agccattgca gaaaatcctc gtagcttttc tcgcccgagc cgagcgcgac aaaatacgcg
      721 ccggaagcga taatatcgcc cgcgacctgt tgaatcagct caaagccttt ctgcgccgtc
      781 agccgcgaga tgttcgcaaa gacgggcttg tccaaatcga ctggcaggct gaatctttcc
      841 agcatcgcgc gtttgcattc gcgtttgccg ctcaaatccc gcgcgctata atgcgccggc
      901 agttccgggt cggtttccgg attccagacg tcgtaatcga cgccgttcgt gatgccgatt
      961 aaacgtccgg cgcgctgccg caaaagccat tccaagccgt aaccgttttc ggcggtctga
     1021 atttcctcgg cgtaacggcg cgaaaccgtc gagagcatat ccgccgcgct caaaccggct
     1081 ttcaaagccg aagccgcgcc gtcagacaaa aaagcgttgc gctcgaaatc gctgccgaag
     1141 ccgagcttcc acaattcccc ggcgccgaac acgccctgat acgccatatt gtgaatcgaa
     1201 agcaccgtcc gcgtgttccg ccaatacgaa tcccggaacc ttttcgacgc aatctcgacc
     1261 gccgcaaaac ccgtgtgcca gtcgtttaaa tgaacgatgt cgggcgccgc gccgatgcgt
     1321 ctcaacaaaa tcagcgccgc gtgattaaaa aacgcgaacc gctcgtaatc ttcattaaac
     1381 ccgtaaatcg agctgcgatg aaaatactcc ggcgcgtcga tcaaaaaagt cggcgacccg
     1441 ttcgcctcgc tgtaaaaagc ccgcgcccgc aatgagcgcc cgcgccagtc aacccaaagg
     1501 tcgtcaatcg ccgtgcgcca caaatattcg cccttcgttt gccggtaaca aggcgtaatt
     1561 aaaaccgaat ccacgccgac ctgccgcaaa gccttcggca aagcgcccgc cacatcgccc
     1621 agaccgcccg ttttcgaata tggaactgct tccgcagaaa gaaccgcaac tctcatcgtc
     1681 agttatcatt tatcatttat cgtttacgag ttgtcaaatc tcagaaataa attgcctgcg
     1741 gcttaacgac cggaataaat atcttttcct ctacaggctg aaatgcccgg aaagaaatcg
     1801 cctatccgga gacgccttct tccgccgtat ttccttcctg cgtgcgctgt cgccagattt
     1861 ccgccagcat tccgctgacg gcgacgacga ccggaccgag cacgagccct gccgagccga
     1921 tcagagccag cccgccgatg acggaaaaga agaccagcaa agggtgcatt ctgactttat
     1981 ctccgaccag aaccggataa agcagattat cgaccgcgcc gatgaccagc gctccccagc
     2041 cagccaggag cagagccttg cccggctcgc ccgtcagcag caggtaaacc gccgccggaa
     2101 tccagaccgc cggcgcgccc agaaccggca cgagcgacaa taaaaacatt acgacgcccc
     2161 agagcacggg cgcgggaatg ccgagccacc agaacatcag cccgcccaga aagccctgca
     2221 ccatcgaaac ggcaatcgtc ccgtagagag tggcgtaaat aatcttgccg acgcgggaaa
     2281 acattttgtc gacctccgac tgcgacagcg gcgtcaggga tttgagcgtt ttgacgatca
     2341 ggttcttgtc gcggaaaaaa taaaagagcg tgaaaaaagt gatgagcagc tgcgcgatcg
     2401 tccagattaa gccgcccacg accgtgccca cgtgcgagga cgccgcttcc gccgcgctgc
     2461 cggcggcgct gcgaatatcg aaatgcggct cgacccattt gattagaggc gcgagctttt
     2521 cattgccctg cacggcatcc tgccactgtt ccggcgcggt ctgttttttt aaggtttcgg
     2581 cgcttttacc gatttggctg attaaagttt gtccgacaaa tacgccgggg acgatgattg
     2641 ccagcgtgat aatcaggacg gtgatgcccg ccgcgatatt cgggcgattg attcgcgaaa
     2701 gaatccagcc gtgaagcgga taagccgcaa tcgccagcgc cgtcgcccac acgaccgccg
     2761 gaataaaagg ctgcgctatc agataacaca cataaaaggc aatcgccgtc gccgctatca
     2821 acaccaaaac cagcgcccgc tcgcgcgtca gccagccgcc atcgaccggc gtttcaattt
     2881 tttgtttatc ctgagagtta ttcatctata aataatatga attgaaactc ccaataaaat
     2941 caaacttccc cggaaacaac gacttaggac acagcagaca attttctcat ccatcacgtt
     3001 ttcttaatct ccgcgcaatg agcgcaacgc cttccattta tttttccttg ccggaaaaac
     3061 ataaatcatt aatttaaaaa cgaaatattc ggctcattca ccgctctaat tttttaataa
     3121 tcatttgagc gaattctttc ggagtcatat tttccaaact gatataaccg attgtttttc
     3181 ttaaaccttt aagctccgta ttatcaaacc gcgcgggcaa aacgtattct tttttcgcct
     3241 gaatggcgtt atcgaaagcg ctttgtcttt cgtggtctgt ccacatcttt ttggcgtaat
     3301 gttctgaaat aaacatcacc acatattcgg aacggtttcg ataaatatcg tccaattcgt
     3361 cataaagatt ctttccccat aaattaacct gctcgtattc gtcgtaaaag acttcaatat
     3421 tgttgtcttt taactcttga gcaacttttg aaacatacga acggtcttcg ccagcaaaac
     3481 ttaatgcgac atcatactgt ataagtaaat cttctcttcc ggtaaaagcg aaagggctct
     3541 ttatcattgc gcgtcttaat cttttatttg gtgattcaaa acccttgagc atttccgtaa
     3601 cgtccacttc ggcatcaata tcagggaaat aatgtttctt tatacccttt tcttccagtt
     3661 tcaaaagcga ttcatataaa atcaaaccct tttcgtgctg agcgtactcg gttactttca
     3721 gattcgacat agtttcgtga atcttctcaa actgtcggcg gattatcgaa ataaatcctc
     3781 gccgcgtttc cattttgccg tca
//
LOCUS       DASWNE010000578         1923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_689586, whole genome shotgun sequence.
ACCESSION   DASWNE010000578 DASWNE010000000
VERSION     DASWNE010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689586"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..1185
                     /locus_tag="VGB68_11400"
     CDS             22..1185
                     /locus_tag="VGB68_11400"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0049887.1"
                     /translation="MKFDAIIIGAGAAGLFCAMEAGKRGRKILVIERSAQTGRKILIS
                     GGGRCNFTNVNTKAENFVSQNPHFAKSALARYTPQDFIELVKKHRIQFYEKKLGQLFC
                     KESARQIVEMLLEECRAAKVEIRANCAVKTVNKNALFEIETNQGVFQSESLVIATGGL
                     SFPKIGASDFGYRIARQFGLKITETRASLVPLVFEKKAQNFIRLAGVSVDSTASSGKA
                     SFRENILFTHRGLSGPAVLQISNYWQKEKAVSIDLLPNENALELLEKNHSSRQNLDNF
                     LSRFLPNRFSEVFTAQNFANKPLNQFSRSEIEQIADRLNNWQVKFIETEGYDKAEVTL
                     GGVETGELSSQTLEAKKAPGLYFIGEVVDVTGWLGGYNFQWAWSSAFAAGQAI"
     gene            1358..1864
                     /locus_tag="VGB68_11405"
     CDS             1358..1864
                     /locus_tag="VGB68_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049888.1"
                     /translation="MKKHLLPCGIFILLFSLAAFSQTDAPTPQNQIKNQTTTDPDEKN
                     SDLVITANVRARELKIEIVPNPTVEFPGKPERRTVWEADRENLPEKTEPGVTYRNIGI
                     RLRIYSRFAEIQRIVLEAIDEEPNSTVVNQTSPAETPQDKAATLPSVSPVARKPKSKT
                     NRTGQARK"
BASE COUNT          503 a          498 c          494 g          428 t
ORIGIN      
        1 gaaaacccgc tatctggcgc aatgaaattt gacgcaatca tcatcggcgc gggcgcggcg
       61 ggacttttct gcgcgatgga agccggaaaa cgcgggcgga aaattctcgt catcgaacgc
      121 agcgcgcaga cgggacgcaa aattctgatt tccggcggcg ggcgctgcaa ttttacaaac
      181 gttaatacca aagccgaaaa tttcgtctcg caaaatccgc atttcgcaaa atccgcgctg
      241 gcgcgctaca cgccgcagga ttttatcgaa ctggtcaaaa aacaccgtat tcaattttac
      301 gaaaaaaagc tcggacagct tttctgcaag gaatccgcgc gccaaatcgt cgagatgctt
      361 ctggaagaat gccgcgcggc aaaagtcgag attcgcgcca attgcgcggt gaaaaccgtt
      421 aacaaaaacg ctctgtttga aatcgaaacg aatcagggcg tttttcaaag cgaatcattg
      481 gtgattgcca cgggcgggct ttcgtttccg aaaatcggcg cgagcgattt cggctaccga
      541 atcgcgcggc aattcggctt gaaaatcact gaaacgcgcg cctcgctcgt gccgctcgtc
      601 ttcgaaaaaa aagcgcagaa ttttatcagg ctcgcgggcg tctccgtcga ttcgaccgcg
      661 tcgagcggca aagcttcgtt tcgcgaaaat atcctgttta cgcatcgggg gctttccggt
      721 cccgccgttc tgcaaatttc caattactgg caaaaggaaa aagccgtttc cattgattta
      781 ttgccgaacg aaaacgcgct cgaactgctg gaaaaaaacc attcgagccg gcagaatctg
      841 gataattttt tgagccgttt tctgccgaac cgattctcgg aagtctttac ggcgcagaat
      901 tttgcaaaca agccgctgaa ccagtttagc cgcagcgaaa tcgagcaaat cgccgaccgg
      961 ctgaacaact ggcaggtaaa atttatcgaa accgaaggct acgacaaagc ggaagtgacg
     1021 ctcggcggcg tcgaaacggg cgagctttcg tcgcagacgc tggaagcgaa aaaagctccc
     1081 ggactttatt ttatcggcga agtcgtggac gtgacgggct ggctcggcgg ctacaatttt
     1141 caatgggctt ggtcgtcggc tttcgccgcc ggtcaggcga tttgaaagac gcgcgccgat
     1201 tttcgatgct cgaatttaaa gttcggattt gcagccttcg gttctatttc cgggcgctgt
     1261 tcgttaaaaa aaaacaattt actaaaaaat gggcttttgt tatgcttgaa gcgtatcgtt
     1321 tttgtttttt acgatttttc agaacaaggc agggtttatg aaaaaacatc ttcttccgtg
     1381 cggaattttc attctgctgt tttcgctcgc agcgttttcc caaaccgacg cgccgacgcc
     1441 gcaaaatcaa attaaaaatc aaaccacgac cgacccggac gagaaaaatt ccgatttggt
     1501 gattacggca aacgtccgcg cgcgcgagct gaaaatcgag attgtgccga atccgaccgt
     1561 ggaatttccg ggcaagcccg aacgccggac cgtctgggaa gccgaccgcg aaaatctgcc
     1621 cgaaaaaacc gagcccggcg tgacgtatcg aaatatcggc attcggctgc gtatttacag
     1681 ccgtttcgcc gagattcagc gcatcgtgct ggaagcgatt gacgaagaac cgaattcaac
     1741 cgtggttaat caaacttcgc ctgcggaaac gcctcaggac aaagccgcca cgctgccgtc
     1801 cgtcagcccg gtcgcccgaa agccgaaatc caaaacaaac cgaaccggac aagcgagaaa
     1861 ataaggagaa cccgatgaat tcaaccgcgc gtttacgcgt tttgtttttc gcctgtgttc
     1921 tgt
//
LOCUS       DASWNE010000579         6026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_55267, whole genome shotgun sequence.
ACCESSION   DASWNE010000579 DASWNE010000000
VERSION     DASWNE010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55267"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="VGB68_11410"
     CDS             complement(<1..942)
                     /locus_tag="VGB68_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049889.1"
                     /translation="MSAIKQTERVPRPASSPTRQLKIGKWVESLKTSDVNQLWSELHR
                     VVCSHPLVRASKSAGLLVEEGKYNAYTDLTQELFVALLSKERFQHYLDSEMTNQEIEA
                     EISQIELTNLLTAELRKRYPESYRLARRISTLIQTSSTFKRFDNLNNADIHRRLADRT
                     YGLAEWADKKSRRNHQEAEQRVQTVAFHKRDTRMVGCTGDAQIVISNTDLEKLIIRVL
                     KAVDSPIDVRSLRSLVMSRLPVMDIYLVPIGGGDDSDEDNKFQLDPADTRENPEQNLL
                     RQEGENIAAGYVENFLKNLNKSVRGKAKQYNRMISVLW"
     gene            1462..1785
                     /locus_tag="VGB68_11415"
     CDS             1462..1785
                     /locus_tag="VGB68_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049890.1"
                     /translation="MFSLRMVNFRKNPECPASQELLAYQNGELSPRDQIDIHRHIIDC
                     EFCEAEVDLYADFPLAAEEECHSTVGEIPHALYELAEALLERRYADNSLLNKLLNENE
                     KLSKL"
     gene            complement(1917..3749)
                     /locus_tag="VGB68_11420"
     CDS             complement(1917..3749)
                     /locus_tag="VGB68_11420"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEY0049891.1"
                     /translation="MSVAPNLIGFENLPQTIPHYILESLRRNRKPDALSHKIHDAWQR
                     FSGERIIERIRHLTLGLADLGIRAGDRVAIISENRPEWSLTDLAILSLKAVNVPIYTT
                     QAVDQIRYILENSGARVLFVSGKKVFSHAGAALEGVEQLEKVVFFDEEAVPAQEDKYL
                     TCAALEKRGEQLDKIDEKAFERLLDKLDAKDLATVIYTSGTTGEPKGVMLTHENFTSN
                     IMAISKGLPIRNDDRSLAVLPLSHIFERCVFYVLCSNGVAIHYCASFDQLAGMLGEVR
                     PTVMTAVPRLFEQVYHKIVKKGMAAGGWKTKLFQWALQIGQDYWTAKEEQKRVPATLA
                     AKHALASRLVFSKWREGVGGAIRFFVSGGAPLSKKLSYAFWAAGIPILQGYGMTEACV
                     VSANRPEDSKTGSIGTPFEGVEIKIAEKDGEILVRGRNVMSGYYNQPEETAKALDAEG
                     WYHTGDVGYKDQDGHFYVTDRLKDLFKLSNGKYVAPLQVESLLKQSPLVSQAVAVGSG
                     RKQVGALIVPDWDALKQTLEAEGIAAKGTKEELCENPHVVKRMQKDAVEFTREMNEYE
                     RVKRVYLLPREFSIDKGEMTPTLKIKRSVIDEKYEEAIDEICGS"
     gene            complement(3746..4174)
                     /locus_tag="VGB68_11425"
     CDS             complement(3746..4174)
                     /locus_tag="VGB68_11425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HEY0049892.1"
                     /translation="MDKKELLEKTLDSELLRFLGVRVESVEAEKVVLSMQVAPHVHQY
                     IGIMNGGVSLVLAETAASIGAVMRSDLSKVAPVGIEINANHLRAVSRGKVTALATSLY
                     HGKTISVWQIEIKDERQRLVCVSRCTIALRKGGADLIDKK"
     gene            4353..5420
                     /locus_tag="VGB68_11430"
     CDS             4353..5420
                     /locus_tag="VGB68_11430"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEY0049893.1"
                     /translation="METRNIIVIGASAGGFEAMKKLVAGLPADLQASLFIVWHMPPDV
                     RGVLPQVLSKSGRLPAEHAVDKEPIAPNRIYVAPPNFHLLIERGRVRVTKGPKENRFR
                     PAVDPLFRSAAYAYGTRVIGVVLTGALDDGTSGLWTIKHLGGTAVVQHPEDAEVPSMP
                     ENALREVEVDYVVPVSEMADLLVRLVEEPLETSEVVMEENKQTEFEIKIAAEDSAFES
                     GIMNFGSLTPFTCPDCHGVLFELKDGKRSRFRCHTGHAFSSDSLLTTVTESIEDSLWS
                     AIRGIEESVMLLNHMGEHFANQNQTHLASLYFKKAHEALDRVEIIRQAVMSHERLSND
                     SLRQQAIENGDGANGKKSAAE"
     gene            5521..>6026
                     /locus_tag="VGB68_11435"
     CDS             5521..>6026
                     /locus_tag="VGB68_11435"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEY0049894.1"
                     /translation="MKKKTTAKAETETNSASDSKSALKKDAPAAEPTAKKSFLVVGLG
                     ASAGGVKALQEFFSTMPPNSGMAFAVVLHLSPEHESNLATILQNHTAMPVIQVNETHK
                     VERNHIYVIPPNRQLEMVDGTVRCTESVEKLGARIAIDVFFRTLAEAYERNAVCVVLS
                     GTGTDGTLG"
BASE COUNT         1394 a         1575 c         1549 g         1508 t
ORIGIN      
        1 ccacagaacg cttatcatcc ggttatactg cttggctttg ccgcgcaccg atttgttcag
       61 attttttaaa aaattttcga cgtagccggc ggcgatattt tcgccttcct gccgaagcag
      121 gttctgttcg ggattttcgc gcgtgtccgc cgggtcgagc tgaaacttat tgtcctcgtc
      181 cgaatcgtcg ccgccgccga tgggaaccag ataaatgtcc atcaccggca ggcgcgacat
      241 caccagcgag cgcaggctgc gcacgtcaat cggcgaatcg accgccttta aaaccctgat
      301 aatcagcttt tccaaatccg tattgctgat gacgatttgc gcatcgcccg tgcagccgac
      361 catccgcgtg tcgcgcttgt gaaaagcgac cgtctgcacg cgctgctcgg cttcctgatg
      421 attgcgccgc gattttttgt ccgcccattc cgccaagccg taggtgcggt cggcgagccg
      481 ccggtgaatg tcggcattat tgagattgtc aaagcgctta aaggtgctgc tggtttgaat
      541 caaagtcgag atgcggcggg cgaggcgata agattcggga tagcgtttgc gcagctccgc
      601 cgtaagcagg ttcgtgagtt caatctggct gatttcagct tcgatttcct gattcgtcat
      661 ttccgaatcg aggtaatgct gaaaacgctc tttgctgagc agcgcgacaa aaagctcttg
      721 tgttaaatcc gtgtaagcat tgtatttgcc ttcttcgacc agaagaccgg cgcttttcga
      781 agcgcgcact aaaggatgcg agcagacgac ccgatgcagt tccgaccaga gttgattaac
      841 gtccgatgtc ttgaggcttt ctacccactt tccgattttt agctgccggg tcggcgatga
      901 cgcaggacgc ggcacacgct ccgtttgttt gattgcgctc atatttacct ttttgatttt
      961 atttagaaca aatttccgcc aatttcatgg tgttttaagc gagatgcgtt tcggcatttt
     1021 ttgatatgga aatctcttcc cgaatgagat tttgctccat ttgcggcaaa acgtctctca
     1081 gttcacgcgg tttcagacat gtagaaaaat ttttataaat taaccgccga gcttttgcag
     1141 ttaatttggc aggtcggtca ggcaggcttt tttaagtctg cactgactac atttaagatg
     1201 ttagcaatta ttttcgattt gggcaacagt tttttgtaat tttttgtttt gtttaacaaa
     1261 tgcgtcaaac ataatgtttt gaatggttta tagagccatt ttcagtcgct caaaaaaaac
     1321 cgaagaaaaa aaatttggat taagtgcgga agttatggaa ggagcgaggc attgtagatt
     1381 tacttttttt gtaacgttta tgttcttccg gcagttttcg tgtaccggca agcgggaaaa
     1441 atttgagtta aaaaatcttt tatgttttca ttaagaatgg tcaattttcg gaaaaatcca
     1501 gagtgtcctg catcacagga attacttgct tatcaaaacg gcgaactctc cccgcgcgac
     1561 cagatagaca ttcatagaca tattatcgac tgcgaatttt gcgaagccga agtcgacctc
     1621 tacgcggatt tcccgctggc ggcggaagaa gagtgtcatt cgacggtcgg cgaaatcccg
     1681 cacgcgctct acgaattggc ggaagcgctg ctggaaagaa gatatgccga caattccctg
     1741 ctcaataagc ttttaaacga gaacgaaaag ctttcaaaac tctaatcaca gcgaaaggaa
     1801 atcggggaaa ttaaggcaat aggaaattag aggaatacaa gaatttcaca tttccttttt
     1861 tcccttaact aaactaattt ccttaatttc cttaatttcc ctatttccct gatttcctaa
     1921 ctgccgcaga tttcgtcaat cgcttcttcg tatttttcgt caatcacgct gcgcttgatt
     1981 ttcagcgtcg gcgtcatctc gcctttatcg atggaaaatt cgcgcggcag caggtaaacg
     2041 cgcttgacgc gctcgtattc gttcatctcg cgcgtaaatt cgacggcgtc tttctgcatg
     2101 cgtttgacga cgtgcgggtt ttcgcaaagc tcttctttgg tgcctttcgc cgcaattccc
     2161 tcggcttcca gagtttgttt cagcgcgtcc cagtccggca caatcagcgc gccgacctgt
     2221 tttctgcccg aaccgacggc gaccgcctgc gagacgagcg gcgattgttt caaaagactt
     2281 tcgacctgta gcggcgcgac gtatttcccg ttcgagagct tgaataaatc cttcagccgg
     2341 tcggtgacgt aaaaatgccc gtcctggtct ttataaccga cgtcgcccgt gtgataccag
     2401 ccttccgcgt cgagcgcttt cgccgtttct tcaggctggt tgtaatagcc gctcatcacg
     2461 ttacgcccgc gcaccagaat ctcgccgtcc ttttcggcga tttttatctc gacgccttcg
     2521 aacggcgtgc cgattgagcc ggttttgctg tcttcgggtc gatttgccga aacgacgcag
     2581 gcttcggtca tcccgtagcc ctgcaaaatc gggatgcccg ccgcccagaa cgcatacgaa
     2641 agctttttcg acagaggcgc gccgccggag acgaaaaacc ggatagcgcc gccgacgcct
     2701 tcgcgccatt tcgaaaaaac cagacgactg gcgagcgcgt gtttcgccgc caaagtcgcg
     2761 ggaacgcgtt tttgttcttc cttcgccgtc cagtaatcct gcccgatttg cagcgcccat
     2821 tgaaaaagct tcgttttcca gccgcccgcc gccattcctt ttttgacaat cttgtgataa
     2881 acctgctcga acaggcgcgg cacggcggtc atcacggtcg ggcgaacttc gccgagcatt
     2941 ccggcgagct ggtcgaacga ggcgcagtaa tgaatcgcga cgccgttcga gcaaagcacg
     3001 taaaaaacgc aacgctcgaa aatatgcgat aaaggcaaaa cggcgagcga gcggtcgtcg
     3061 ttgcgaatcg gcaagccttt cgagattgcc ataatattcg acgtaaaatt ttcgtgcgtg
     3121 agcatcacgc ctttcggctc gccggtcgtt ccgctggtgt atatgacggt cgccaaatcc
     3181 tttgcgtcaa gcttatccaa aagccgttcg aaggcttttt cgtcgatttt gtcgagctgc
     3241 tcgccgcgtt tttccagcgc ggcgcaggtc aggtatttgt cttcctgcgc ggggacggct
     3301 tcctcgtcga aaaagacgac tttttcgagt tgctcgacgc cttcgagcgc cgctccggcg
     3361 tggctgaaaa cctttttgcc ggagacgaac aggacgcgcg cgccggaatt ttccagaatg
     3421 tagcggattt ggtcgacggc ttgcgtcgtg taaatcggca cgttgacggc tttcagactc
     3481 aagatagcca aatcggtcag cgaccattcc ggtcgatttt ccgagataat cgccacgcgg
     3541 tcgcccgccc tgatgcctaa atctgcaaga ccgagcgtca gatgccggat tctttcgata
     3601 atccgttcgc cggagaatcg ctgccacgcg tcgtgaatct tgtgactcag cgcgtcgggc
     3661 ttccggttgc gtcgcagcga ttccagaatg taatgcggaa tcgtctgcgg aagattttca
     3721 aaaccgatta aatttggagc tacactcatt ttttatcaat caaatcagcg ccgcctttgc
     3781 ggagcgcaat cgtgcagcgc gaaacgcaca ccaggcgctg ccgttcatcc ttaatttcaa
     3841 tctgccaaac gcttatcgtt tttccatgat acaacgaagt tgccagagcc gtcacttttc
     3901 cgcgactgac cgcgcgcaga tgattggcgt ttatctcgat gccgaccggc gcgactttcg
     3961 ataaatcgct gcgcatgacc gcgccgattg acgccgccgt ttccgccagc accagcgata
     4021 cgccgccgtt cataatgccg atgtattgat gaacgtgcgg cgcgacctgc atcgaaagaa
     4081 cgactttttc cgcctcgacc gattcgactc ggacgccgag aaagcgcaaa agctcgctgt
     4141 ccagcgtttt ttccagaagc tcttttttgt ccataaattt gtcgtcgctg aaatcatagc
     4201 tttgccggcg tttaatgtaa acgagtcgcc ggaatcccgg tcggattttt ttacgcctga
     4261 tttattaagt aataattgtt acggaaagat aagtttgcac ctgtaataat atgataaggt
     4321 aagggtaaat tgtttgttaa ttattttata aaatggaaac acgaaatatc atcgttatcg
     4381 gggcatcggc gggcggtttt gaggcaatga aaaaactcgt cgcgggcttg ccggcggatt
     4441 tacaggcttc gctatttatc gtctggcata tgccgccgga cgtgcgcggc gttttgccgc
     4501 aggttctcag caaatcagga aggctgccgg cggagcacgc cgtcgataaa gagccgattg
     4561 cgccgaatcg aatttacgtc gcgccgccga attttcatct tctaatcgag cgcgggcgcg
     4621 tccgcgtcac gaaaggaccg aaagaaaatc gttttcgtcc ggcggtcgac cctttgtttc
     4681 gctccgccgc ttacgcttac ggcacgcgcg tcatcggcgt cgttttgacc ggcgcgctcg
     4741 acgacggaac ttccggcttg tggacgatca agcatctcgg cgggacggcg gtcgtccagc
     4801 acccggaaga cgccgaagtt ccttcgatgc cggaaaacgc gctgcgcgaa gtcgaggtcg
     4861 attacgtcgt gccggtttcg gaaatggcgg atttgcttgt gcgtttggtc gaagagccgc
     4921 tggaaacttc tgaagtagtt atggaagaaa acaaacaaac cgaatttgaa attaaaatag
     4981 ctgccgaaga cagcgcgttc gaatcgggca ttatgaattt cggctcgttg acgcctttta
     5041 cgtgcccgga ttgtcacggc gttcttttcg agctgaaaga cggcaagcgc tcgcgtttcc
     5101 gctgccacac gggacacgcc ttttcttccg acagcctgct gacgaccgtt acggaaagca
     5161 tcgaggacag tctatggagc gcgattcgag gcatcgagga aagcgtgatg ctgctcaatc
     5221 atatgggcga gcatttcgcc aaccagaatc aaactcatct ggcatcgctg tatttcaaga
     5281 aagcgcacga agcactcgac cgcgtcgaga ttatcagaca ggcggtgatg agtcacgagc
     5341 ggctgagcaa cgacagccta cggcagcaag ctattgaaaa cggcgacggc gcaaacggaa
     5401 aaaaatcagc cgcagaataa aagccgggcg gcgcgtgtat agactagaga catcaaatac
     5461 tgttggagcc ggaaacccga ccgtaaaaga caatataaga cacctgcaac aatatttgtt
     5521 atgaaaaaaa agacgacggc aaaagccgaa acggaaacaa attccgcatc ggactcaaaa
     5581 tctgccttaa agaaagacgc accggcggct gaaccaaccg ccaaaaaatc ctttttagtc
     5641 gtcggcttgg gcgcgtcggc gggcggcgtc aaggctttgc aggaattttt ctcgacgatg
     5701 ccgccgaaca gcgggatggc gtttgccgtc gttctgcatc tttcgcccga acacgaaagc
     5761 aatctcgcga cgattcttca aaatcatacg gcgatgccgg tcatacaggt caacgaaact
     5821 cacaaggtcg agcggaatca catttacgtg attccgccga atcgccaact ggaaatggtc
     5881 gacggcactg tccgctgcac cgaatcggtc gaaaagctag gcgcgcgcat cgccatcgac
     5941 gtatttttcc gcacgcttgc cgaggcttac gagcgaaacg ccgtctgcgt cgttttgagc
     6001 ggaaccggca cggacgggac gctcgg
//
LOCUS       DASWNE010000580         3408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_89569, whole genome shotgun sequence.
ACCESSION   DASWNE010000580 DASWNE010000000
VERSION     DASWNE010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89569"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(271..1251)
                     /locus_tag="VGB68_11440"
     CDS             complement(271..1251)
                     /locus_tag="VGB68_11440"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY0049895.1"
                     /translation="MKKLLRFSLYTIAAILSIFFLLVFYVVYFLPRVPVENIKIEITP
                     GRVERGKYLAENVAQCLDCHSTRDWSKFSAPAVPGSEGKGGEVFDRTMGFPGSYYAPN
                     ITPFHLKDWSDGELFRAVTSGVSRDGRALFPIMPYPSYGKMDREDIYSIIAYIRTLKP
                     VENTPPASESNFPMNIIIHTIPAENALSPKPAPDDTVNYGKYLVNAASCAECHTPARR
                     GQIIEEKIFSGGRYFEMPNGTVISKNITPDRETGIGAWTRQDFITRFKAFENAAHYAA
                     EDLQAGDFNTIMPWSNYAEMTDADLAAMYAYLMSVKPISNKVEKVFIKKQ"
     gene            1498..1914
                     /locus_tag="VGB68_11445"
     CDS             1498..1914
                     /locus_tag="VGB68_11445"
                     /inference="COORDINATES: protein motif:HMM:NF021759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HEY0049896.1"
                     /translation="MLLLLLILANPTATIHPPDEKVVISDDRFARDENLQAKSDALLS
                     LFDDMPPVPVYLTDELFLESGTNTEKGSAYTHCYGHEHPTIFVKKIFYQKANRKQLVN
                     LLKHEMTHAWLCRQRLMSGHDANFRRKFREIGGVGN"
     gene            complement(2071..2850)
                     /locus_tag="VGB68_11450"
     CDS             complement(2071..2850)
                     /locus_tag="VGB68_11450"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY0049897.1"
                     /translation="MKKIKLLVLCLFAACAASCSSATPMQDAFKRTHPVTNVPKSIQN
                     SKPDAHFTDEKAIIVSLLYPEELYVGEDTYQKEDAREIIERRLEKNPSEKQLIYFNAG
                     ANAEYGNIVQILDIIRRVNPENIGLIVEPATGENKKLQVLKVKVSAEPKEEDPLDLLD
                     KRLVINLQKDGKIKLGRFDKYAFKSAAPDIAEQEVSAKLAPMLKENEEKKINLKGTNE
                     IDKTVFVKASRSRLYGEIARLVDAALGAGASEVYLMLDDLD"
     gene            complement(3075..>3408)
                     /locus_tag="VGB68_11455"
     CDS             complement(3075..>3408)
                     /locus_tag="VGB68_11455"
                     /inference="COORDINATES: protein motif:HMM:NF044806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049898.1"
                     /translation="FSHKKQSRKGVHTLDIAKRLIDYGFHPMTIYFPLIVEGAMLIEP
                     TESVGRQDLDSFIEAMKDINREAQENPQLVIDAPHTTRIGRLDEATAARKPVLRWKPD
                     MEATAKTA"
BASE COUNT          789 a          816 c          891 g          912 t
ORIGIN      
        1 atagcagcag cggattttcc gccgcacagt ttggcacaaa cggcgatttg cccgcgccga
       61 atgctttcgt tcgctaattt aacggcgaca tcaaaaatgt cgctccatta aagaaaaaga
      121 gctcgtttaa attaatttaa acgagctctt tttcttattt cacactgtat tcagggcgct
      181 ctgaacggta agtccggctt ccggcttttt ggacggcttc gggacagata cgggcgacgg
      241 gcgcgcggcg ttccggtttc gccttaaaaa ttattgtttt ttaataaaaa ctttctcgac
      301 tttatttgag ataggcttga ccgacatcag ataagcatac atcgcggcta aatcggcgtc
      361 ggtcatttcg gcgtagtttg accacggcat aatcgtgttg aaatcgcccg cttgcaggtc
      421 ttcggcggca taatgggcag cgttttcgaa ggctttgaag cgcgtgatga aatcctgccg
      481 ggtccacgcg ccgatgccgg tttccctgtc gggcgtgatg tttttagaga tgaccgtgcc
      541 gttcggcatt tcgaaatatc tgccgccgga gaagattttt tcttcgatga tttgaccgcg
      601 acgggcgggc gtgtggcatt cggcgcagga agcggcgttg accaggtatt tcccgtagtt
      661 gacggtatcg tccggcgcgg gtttcggcga taaggcgttt tccgcgggaa tcgtgtgaat
      721 gatgatgttc atcgggaaat tcgattctga cgcgggcggc gtgttttcga cgggcttcag
      781 cgttcggatg taagcgatta tcgaatagat atcttctctg tccattttgc cgtagctcgg
      841 gtaaggcatt atcggaaaaa gggcgcgacc gtcacggctg acgccggaag tgacggcgcg
      901 aaacagctcg ccgtccgacc agtctttcaa atgaaaaggc gtgatgttcg gcgcgtaata
      961 gctgccggga aagcccatcg tgcggtcgaa gacttcgccg cctttgcctt cgctgccggg
     1021 aacggctggc gcggaaaact ttgaccagtc gcgcgtcgaa tgacaatcca gacattgcgc
     1081 tacgttttcc gccagatatt ttccgcgctc gacgcgtccg ggcgttatct cgattttgat
     1141 attctcgacc ggaacgcggg gcagaaaata gacgacatag aaaacgagca ggaaaaaaat
     1201 cgacagaatc gcggcgattg tgtaaagcga aaatctcaaa agttttttca tatgcgaaat
     1261 gcgggaaacg gtttaagcgg aacgcatcgt ttataaattt aacgcccgta gtttgtcaaa
     1321 tgaattctca aagcgtcggc ttcataatca tctcgcggtg atataattat ttcggctcaa
     1381 acggcgatgc cgagccgctc ttatatcaat aaaactattg gttacggcag gcgggaaatg
     1441 gttaaaacag cttcggtaat aataaatttc aacggcgctg tttttaaggc gctgctgttg
     1501 ctgttattac tgttgatatt ggcgaatccg acggcgacaa tccatccgcc cgacgaaaag
     1561 gtcgtgattt ccgacgatag gtttgcgcgt gacgaaaatc tacaggctaa gagcgacgct
     1621 ttactgtctt tattcgacga tatgccgccg gttcccgtct atctaactga cgagctgttt
     1681 ctggaatcgg ggacaaatac cgagaaaggc tccgcttaca cgcattgtta cggtcacgag
     1741 catccgacca tctttgtaaa aaaaatcttt tatcaaaaag ccaaccggaa acaactcgtt
     1801 aaccttttga agcacgagat gactcacgcc tggctgtgcc ggcaacgcct gatgtcgggg
     1861 cacgacgcta attttcgccg gaaattcaga gagattggcg gagtagggaa ttagccggaa
     1921 tacgactgaa acgggctatc tttttaaatc gtcgggttaa acataagctt tgcttttaag
     1981 tgtcgaagac cggcgcttac cggaaagcaa aaaaccggaa gcgtttttcg attcttccgg
     2041 ctcgggtctt gaacgaataa aaacagcggg ttaatccaaa tcgtccagca ttaaataaac
     2101 ctccgacgcg cccgcgccga gcgccgcatc gacgaggcgc gcgatttcgc cgtagagtct
     2161 ggagcgcgac gccttgacga aaacggtctt gtcgatttcg ttcgtgcctt tcagattaat
     2221 ctttttctct tcgttttcct tgagcatcgg cgccagtttg gcgctgactt cctgttcggc
     2281 gatgtccggc gcggcgctct tgaaagcgta tttatcaaac ctgccgagct ttattttgcc
     2341 gtctttttgc agattgataa ccagacgttt gtcgagcaaa tcgagcgggt cttcctcttt
     2401 cggctcggcg gaaactttca ctttcagaac ctgaagcttt ttgttctcgc cggtcgccgg
     2461 ttcgactatc aatccgatgt tttccggatt gacgcggcga atgatgtcca atatctgtac
     2521 gatgtttccg tattcggcgt tggcgcccgc gttaaaatat attaactgtt tttcggacgg
     2581 atttttttcg agccgccgtt cgattatttc acgcgcgtcc tctttctgat aagtatcttc
     2641 gccgacgtac agctcctccg ggtaaagcag cgaaacgatg atcgcctttt cgtccgtaaa
     2701 atgagcgtcg ggctttgaat tctgtatact tttcggaacg ttggtgactg gatgcgtgcg
     2761 cttaaaggcg tcctgcatcg gcgttgcgct cgaacacgac gcggcgcagg ctgcaaacag
     2821 acaaagaacg agtaacttga tttttttcat gctcttttca gctcgatttt gaaatggaat
     2881 ttttccggat tgtagtggga actgagatat attgcctgtg tttaaaacaa aggtcaacag
     2941 caagcgataa aattgtgaaa ctaaagaaaa aagcttggaa gaagaaaagc ggcgcgcaat
     3001 tttaattgcg cgccgctttt cgccttttat ttattaaacc gcaggactga ccaaagcaga
     3061 cagcttccgg ctgattacgc cgttttcgcc gtcgcttcca tatccggttt ccagcgcagg
     3121 acgggttttc gcgcggcggt ggcttcgtcc agccgtccga tgcgcgtcgt gtgcggcgcg
     3181 tcgatgacga gctgcggatt ttcctgcgct tcccggttaa tgtccttcat cgcctcgata
     3241 aacgagtcca aatcctgtct gccgacggat tcggtcggct cgataagcat cgcgccttcg
     3301 acgattaaag gaaaataaat cgtcatcggg tgaaagccgt aatcaatcag tcttttagcg
     3361 atgtctaatg tgtgaacgcc tttgcgcgac tgctttttgt gcgaaaag
//
LOCUS       DASWNE010000581         4552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_196728, whole genome shotgun sequence.
ACCESSION   DASWNE010000581 DASWNE010000000
VERSION     DASWNE010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196728"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VGB68_11460"
     CDS             complement(<1..552)
                     /locus_tag="VGB68_11460"
                     /inference="COORDINATES: protein motif:HMM:NF022030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049899.1"
                     /translation="MLYYLLYKLIHLGQCANGCDSYFFKVLNVVQYVTFRTGMVTITA
                     LLISLLLGNKVIKKLEALKFGQEIREEGPQGHHKKKGTPTMGGILIIGSVIISTLLWA
                     RLDSLYLWIALVATTLFAAVGFADDYIKIVKKRSLGLTGKQKLAGQLVAAVGVWLVLY
                     MWGSYPWNLSIPFFKATAIPDGIT"
     gene            complement(627..2078)
                     /gene="murF"
                     /locus_tag="VGB68_11465"
     CDS             complement(627..2078)
                     /gene="murF"
                     /locus_tag="VGB68_11465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEY0049900.1"
                     /translation="MKLETAAKYMDADASDTNPALLQTEFKTAVIDSREVQAGDVFFA
                     LSQPDYKNNCFNDEFADSHKYIPSAFEKGALACVARPDRFEEHRSALEQFKDRLLLVD
                     DAILALQMLAKGVYEEWNKPVVAVTGSAGKTTAKTLITGVLAARRKVLTNIKNYNNGL
                     GHPLTVMRLLAEPDYEVAVLEMGMSTPMNEIARLCRITPPDVAVELNVLPVHVEHLGS
                     IENVAKAKAELVEGMKTGGTAVLNADDFRVLAMRDLSRGETLTYGIETPADVMAKDIR
                     FDHFGETVFTLQTPGDAAEVLFPLNGRHNISNALAAAAVGFLFGMTAQEIAAALHTVA
                     PPPQRGEILRFQAGFTVVNDSYNSNPDALLSMVQTLVDGGGSAKRKIVVAGEMRELGE
                     SSAQIHRETGKRIAEIGIDKLFGVEGFAEKLLEGARAAGLSDVEFYENSLTAGERLVD
                     EIQAGDLVLIKGSRGVRTEKVVEKLLEKFELEK"
     gene            complement(2075..4231)
                     /locus_tag="VGB68_11470"
                     /pseudo
     CDS             complement(2075..4231)
                     /locus_tag="VGB68_11470"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
     gene            complement(4266..>4552)
                     /locus_tag="VGB68_11475"
     CDS             complement(4266..>4552)
                     /locus_tag="VGB68_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049901.1"
                     /translation="RIKLNREITLSPVEIKKAARKIGFREMTASNIDVSSRKPADKPK
                     PEALSKPQNENANKEIARAEKKEEKKIAKTAKEETKKTETKTDRKKESAR"
BASE COUNT          881 a         1310 c         1093 g         1268 t
ORIGIN      
        1 ggtgatgccg tccggtatcg ccgtcgcctt gaaaaacggg atactcagat tccacgggta
       61 gctgccccac atataaagaa caagccagac gccgactgcc gccaccagtt gcccggcgag
      121 cttttgcttt ccggttaagc ccagactgcg ttttttgaca atcttgatgt aatcgtcggc
      181 aaagccgacc gccgcgaaaa gagttgtcgc caccagcgca atccacaggt aaagactgtc
      241 gagccgcgcc cagagcagcg tcgaaatgat gaccgagccg attatcaaaa tgccgcccat
      301 cgtcggcgtg ccttttttct tgtggtgccc ttgcggacct tcttcgcgga tttcctgccc
      361 gaatttcagc gcctcgagtt ttttaataac cttgttgccg agcagcagcg aaatcagcag
      421 agcggtgatg gtcaccattc cggtccggaa cgtaacgtac tgcaccacgt tcagaacttt
      481 gaaaaaatag gaatcgcagc cgtttgcgca ttgcccgaga tgaatcagct tatacaaaag
      541 gtaataaagc atcgtttgga tttgcgaatt tggatttgcg attagcgccc agtcgccgct
      601 ttatcacctt tccgcatgcc gcgttttcat ttctccaatt caaatttttc caacaacttc
      661 tcgaccacct tctccgtccg cacaccgcgc gaacctttaa ttaaaaccaa atcgcccgcc
      721 tgaatttcat cgacaagcct ttcgcccgcc gtcagcgagt tttcgtaaaa ttcgacgtcg
      781 cttaaacccg ctgcccgcgc tccttcgagc agcttttcgg caaaaccctc gacgccgaaa
      841 agcttgtcga taccgatttc ggcaatcctt tttcccgttt cgcgatgaat ctgcgccgag
      901 ctttcgccca gctcgcgcat ttctccggcg acgacgattt tgcgttttgc cgacccgccg
      961 ccgtcaacca gagtctgcac catcgaaagc agcgcgtccg ggtttgaatt gtaggaatcg
     1021 ttaacgaccg taaatccggc ttgaaaacgc agaatttctc cgcgctgcgg cggcggcgcg
     1081 accgtgtgca gagcggcggc gatttcctgc gccgtcattc caaacaaaaa accgaccgcc
     1141 gccgccgcca gcgcgttgga aatgttgtgc ctgccgttaa gcggaaacaa aacctccgcc
     1201 gcgtcgcccg gcgtctgcaa agtaaagacg gtttcgccga aatggtcaaa gcgaatatct
     1261 ttcgccatca catcggctgg cgtttcgatt ccgtaagtaa gcgtttcgcc tcgcgacaaa
     1321 tcgcgcatcg ccagaacgcg aaaatcgtcg gcgtttaaga cggctgttcc gccggttttc
     1381 attccttcga ccagctcggc tttcgccttc gccacgtttt caatcgagcc gagatgctcg
     1441 acgtgaaccg gcaaaacgtt taattcgacc gccacatcgg gcggcgtaat gcggcaaagg
     1501 cgcgcgattt cattcatcgg cgtggacatt cccatttcca gaacggcgac ctcgtaatcc
     1561 ggctcggcga gcagacgcat aaccgtcaaa ggatgtccca gcccgttgtt ataattttta
     1621 atgttcgtca aaaccttgcg ccgcgccgct aaaaccccgg taatcaaagt tttcgccgtc
     1681 gttttcccgg cgcttcccgt gacggcgacg accggcttgt tccattcttc gtagacgcct
     1741 tttgccagca tctgcaaagc gagaatcgca tcgtcgacca gcaaaagccg gtctttaaat
     1801 tgttccagcg cgcttcgatg ttcttcgaat ctatcgggac gcgcgacgca ggcaagagcg
     1861 cctttttcaa atgccgacgg aatgtatttg tgcgaatccg caaactcgtc gttgaaacag
     1921 ttgtttttat aatccggctg cgaaagcgca aaaaacacgt cgcccgcctg cacctcgcgc
     1981 gagtcaatca ctgccgtttt gaactcggtt tgaagcagag cgggattcgt gtcggacgcg
     2041 tcggcgtcca tatattttgc ggcggtttcc agtttcaagt tttggcttct atcctctcaa
     2101 aaaatgtttc gacgtccgcg ctgcggcgcg ccgaggaaat gctttcgtaa atcagcttgt
     2161 atttcacaaa gcgatggctc gaaatactgt ttctgttttt cgtggtcgtt ttcgactttt
     2221 cttttttctg cgttcccgct tcagggcttt tcaatttttc tttttctcca gacatttttt
     2281 tatctttcga ttctttcgct ttatcgctct tttcaatcga ttcctttgtt ttgtcaccct
     2341 tttcattttt tccaacccct ttttcgtcaa tctttttgtc cgttttttcg gctggtttat
     2401 tcatcgccga aggcagctcg atttccgtct cttgcggaat gttttcggct gtcaaatccg
     2461 tttgaatcgc gccgtccggc acgacgttca attccggcag aacgctttcg gcgatttcgc
     2521 ggaaaatcgg tgcggaaacc tgcccgccgt cgcgcgctac gccctgcggc tcgtccagaa
     2581 cgacggcaat cacgaccgac ggattatcgg cgggcgcaaa accgacaaac gaggaaacgt
     2641 atttgttgcc gctcactctt ttcagcttcg cgtcgtattt ccaagccgtt cccgtttttc
     2701 cggcagacgt atagccgttc agacgcgcgc gtttgcccgt tccgcttaaa acgacctcgc
     2761 gcagcatctg ccgcagcgcg cgcgcagttt cagcgttgac aaccggcact cgctcgagtt
     2821 ccgctgcctg aatatttttc tcttcgctgc tgcttcgaat ttcttttatg atgtgtggtt
     2881 taactcttac accgtcattg gctatagtcg caaaagccgc cgccgtttgc aaagccgtca
     2941 catttatttc gtagccgatg gacatcgaag ccagagagtc gccgaaccag ctttccggcg
     3001 agcggatttg tcctttcgct tccgccggaa gctcgatgcc ggtcgcctcg ccgaaaccga
     3061 attggcgcgc gtaattgtag aaggtctgct tgccgagcat ctgcccggtt ttaatcgcgc
     3121 cgatattgct ggagacggcg agagcttccg tgtaagaaat cgaattttga cagtgtttat
     3181 cggcaaattc gcgcccgccg accttgatga cgccgccgcc gcaatcaaac attttgcccg
     3241 gcgcgatgag tttttcttcg agcgcgccgc cgtatgtgac gagctagaaa accgagccgg
     3301 gcgaataaag attctgcacc gctttattaa taatcgcgtc cggcgcggct tcgttgaatt
     3361 tgttcgggtc aaacgtcgga taatttgcca tcgccagcac ttcgccggtt ttcgggtcta
     3421 aaacgattgc cgtcccggat ttggcgcgcg ccgcttcgac gcccgatttc aaagcctgct
     3481 cgaccttata ttgaatcgaa ttggaaatcg tcagataaac gtctttcggc ggctcgcgct
     3541 cctcggtttc cgattcgtcg taaactctgc cgagccggtc gcggtcctgc catttttggg
     3601 tcaccgcgcc gcgcagggcg acttcctgcg attgctcgat tccggcttgc ccgacgtcgt
     3661 ccgagttgct gaaaccgacg atgtgcgccg ccagagtgcc ctgcggatac tggcgttttt
     3721 gttcctcgcg ccagtgcaga ccggcgtatt tcggctcgtc ggctttcttt aaattctcga
     3781 ctctcagcgt gtcgtttaat ttctgcgccg cgctttcatc gatttttcga gccagccaga
     3841 caaatttttt gccgctctgt ttcgcttctt ttaaatcctt taaaatttcg ttcggcttaa
     3901 tcttcagcgc gccggcaatc gctttcgccg tcgcttcgac gtcttcgatt tcgcgcgggt
     3961 cggcgaataa ggattttact ttaacgctca tcgccagggc tcgttccgag cggtcgaaga
     4021 tagtgccgcg cagcatcttg cttttctttt cgacgcggcg caaatcctgc gctttttctc
     4081 tcagccattg gtgctgcgtg acctgcaaat gcacgaggcg gacgccgata atgccgatcc
     4141 agaccgcaaa aatcgccacg ataaacaaaa accgtgtaaa ggcggtctgc gccggatttt
     4201 ttttcttttt cactgtcggg cgtttctgca tacttcctaa aacctatttt ataattttat
     4261 aaaatctatc tggctgattc ttttttcctg tctgttttcg tttccgtttt tttcgtttct
     4321 tctttcgctg ttttggctat cttcttttct tctttttttt cggctctggc aatttcttta
     4381 ttggcgtttt cgttctgcgg cttgctcaaa gcttccggtt tcggtttgtc cgccggtttg
     4441 cggctggaaa cgtctatatt gctcgccgtc atctcccgaa aaccgatttt gcgcgccgct
     4501 tttttgattt ccacgggcga aagcgttatt tcgcgattga gcttgatgcg ct
//
LOCUS       DASWNE010000582         5086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_19638, whole genome shotgun sequence.
ACCESSION   DASWNE010000582 DASWNE010000000
VERSION     DASWNE010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19638"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(281..1369)
                     /locus_tag="VGB68_11480"
     CDS             complement(281..1369)
                     /locus_tag="VGB68_11480"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010478.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEY0049902.1"
                     /translation="MFYSLSLMTENGALKILENVSLAPLTTLKIGGAARFFVSAESET
                     QAVEAVRFARENSLEIFVLGGGSNVLISDKGFDGLVLQIALKGISTFQEADKTVRVTA
                     QAGEDWDGFVRFCVEKNLAGVECLSGIPGFVGGTPVQNVGAYGQEVSETIVSVRAFHR
                     KNNQLIELSGAECGFGYRTSIFNTIEKNRYIVLQVTYALKYNGAPKIVYRDLQNFFGD
                     KNPDLAETRAAVLKIRAEKSMVIKADDANCRSAGSFFKNPVVTNKKFSEIVEKAKAFG
                     IRQVPSFSVDDENVKVPAAWLIENSGFQKGFRKGNAGLSTRHTLAIVNTGGANAADIL
                     NLKNEIQHKVKENFDVTLIPEPVFVGFD"
     gene            complement(1394..3235)
                     /locus_tag="VGB68_11485"
     CDS             complement(1394..3235)
                     /locus_tag="VGB68_11485"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0049903.1"
                     /translation="MNNKMQWRKIALTIFVLWTLIFQSIPVGAQEIVTSEDISGGSSV
                     FVFRQGKKTAQTKSAFRNTSVKRNAVARNESRRKVKAQIVAVNKTKPQRTKVKVDPKT
                     VAKTTAQVKTTAQKEKASTALTGAAEVYLERNQIDSAIDYFRQALALNPKNENARLGL
                     SEALTAKGDETSAKVNPQASLVFYEEAVKLNDKNAAAFASLGEIYDDSNQNEKAIANY
                     EKALQLSPELSELHAPLGILYLQKGEVALAENYLSKATAVNGANDPETQYFKGLLYYK
                     QNKNTEALAAFSGAIKAKPDYAEAYYYQGETYDRLDKDKEAINAYKEAVRINPKFTEA
                     WFDLGVAYYNRNRYEDAITAYKETLKLKNDNGEAHLNLANAFRQLDRYAEANGEYGLA
                     AQFIKGDADMYSEWGFCLGKERKWNKAVERLDTAVAMRPDPVDHTNLGWAYYNSAQDD
                     LRAKREADAKVKLAKGRDSLQKATVLNPNFDAAFLNLGITLTDLGDYQGAVQALTRAG
                     NLRKNWISAINELGLAYRQLNDFDNAVKQFKKATDIDKKFAAGFYNLGEAEYRRGNTK
                     EARKAQEKLKKLNPNLATQLDIVVSGAVLNRTQNEIKNRIPKPKLPF"
     gene            3536..4297
                     /locus_tag="VGB68_11490"
     CDS             3536..4297
                     /locus_tag="VGB68_11490"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY0049904.1"
                     /translation="MQEKVAIVTGASSGIGRATALLFAEKGIKVVGVGRSEKELNALR
                     QQAKEKNGIVKTHLADLLENKQVERVIAETAETLGGIDILVNAAGIIANGSIETTALE
                     EWDKMMNINLRSVFFIMQKCVPHLEKTRGNIVNVSSVTGVRAFPNVLAYCVSKAAVDQ
                     LTRCSALELAPKGVRVNAVNPGVVVTNIHKRGGMDEEKYEQFLEHSKTTHPLGRVGNP
                     SEVAELIYFLASENASWITGATYEIDGGRAQTCAR"
     gene            4479..>5086
                     /locus_tag="VGB68_11495"
     CDS             4479..>5086
                     /locus_tag="VGB68_11495"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HEY0049905.1"
                     /translation="MKVQLLPTTFDRSGRATAQQHLCCFVIDDRVAIDAGSLAMATTP
                     EQKQNIRDVVLTHAHLDHIAGLPLFIDDLFATLENPVRVFAAPEVIEILERDIFNWDV
                     YPRFSELQNKFGEVLQYEAFTDGKEFSAAHLRIKAVKVNHKVPTVGFIICDGNSKIAV
                     SGDTAQMDVFWQVLNEEENLSALLIECAFPNELEDLANASHH"
BASE COUNT         1197 a         1263 c         1251 g         1375 t
ORIGIN      
        1 ttccaaaaac gcgaaaaaac gcgttaatat cgacaaaatc ccgtaaattt tattattaat
       61 gtgaatcaag cgaagaaata attgtgggtg cgataaagtc gtttgaccgc tctatcgaaa
      121 agctctgctc ttgatttgca cttttacagt gttaattttc gttcggaaaa gacgtttaat
      181 cgaaatctga atgtccgctt tgcccgtcag cagtcgccga atattaaacc acacaatttt
      241 ttgccggaca agaatcgtta tgtaaaattt tgtaaatctt ttaatcgaag ccgacgaaga
      301 ccggttcggg aatgagcgtc acgtcaaaat tttcttttac tttgtgttga atctcgtttt
      361 ttaaatttag aatatcggcg gcgtttgcgc ctccggtatt aacgattgcc agcgtatgcc
      421 gcgtcgataa accggcgttt cctttgcgga agcctttctg aaaaccggaa ttttcaatca
      481 gccacgcagc cggaactttt acgttttcat cgtctaccga aaagctcgga acctgccgaa
      541 ttccaaaagc cttcgctttt tcgacaatct ccgaaaactt tttattggtg accaccggat
      601 ttttgaaaaa cgagccggcg ctgcggcaat tcgcatcgtc tgccttgatg accatcgact
      661 tttcggcgcg gattttcaga acggcggcgc gcgtttcggc taaatcgggg tttttatcgc
      721 cgaaaaaatt ttgtaaatcc ctgtagacga ttttcggcgc gccgttatat tttaaagcgt
      781 atgtgacctg caaaacgatg taacgatttt tttcaatcgt gttgaagatg cttgtccggt
      841 aaccgaaacc gcattcggcg ccgctcaatt cgattaattg attgttttta cggtggaaag
      901 cgcgaaccga gacaatcgtt tccgaaactt cctgcccgta agcgccgacg ttttgcacgg
      961 gcgttccgcc gacaaagccc ggaatgccgc tcagacattc gactccggct aaattttttt
     1021 cgacgcagaa ccggacaaaa ccgtcccagt cttcgcccgc ctgcgccgtt acgcgaacgg
     1081 ttttgtccgc ttcctgaaac gtggaaattc cttttaaagc gatttgtaaa acgagaccgt
     1141 caaagccttt atcggaaatt agaacgttgc tgccgccgcc gaggacgaaa atttcgagcg
     1201 agttttcgcg cgcgaatctg acggcttcga ccgcttgcgt ttcgctttcg gcggagacaa
     1261 aaaaacgcgc cgcgccgccg attttcaaag tcgtcagcgg cgcgagcgat acgttttcca
     1321 ggattttcaa tgcgccattt tcagtcatca gcgacaaact ataaaacaaa tccgttcgcc
     1381 gctcaaacga aagttaaaac ggcagtttcg gtttcggaat gcgattcttg atttcattct
     1441 gcgttcggtt taaaaccgcg cccgaaacaa cgatgtcgag ctgcgtcgcc agattcgggt
     1501 tcagtttttt cagtttctcc tgcgcctttc tcgcttcctt cgtgtttccg cgccgatatt
     1561 cggcttcgcc gagattgtag aaaccggcgg cgaatttttt gtcgatgtcg gtcgcttttt
     1621 tgaactgctt gacggcgttg tcgaaatcgt tgagctgacg atacgccaga ccgagttcgt
     1681 tgatagccga aatccagttt tttcgcagat tgccggctct ggtcaaagcc tgaaccgcgc
     1741 cctgataatc gcctaaatcc gtcagcgtaa tgccgagatt caaaaatgcg gcgtcgaaat
     1801 tcggattcag cacggtggct ttttgcagcg aatctctgcc tttggcaagc ttcactttgg
     1861 cgtcagcctc gcgtttggcg cgtaaatcat cctgcgcgga gttgtaatac gcccagccga
     1921 gattggtgtg gtcgaccggg tcgggacgca tcgcgaccgc cgtatccaga cgctcgacag
     1981 ccttgttcca tttgcgctct tttcctaaac agaagcccca ttcgctgtac atatcggcgt
     2041 cgcctttaat aaattgggcg gctagcccgt attcgccgtt ggcttcggcg tagcggtcta
     2101 actgccggaa agcgtttgcc agattcaggt gcgcttcgcc gttatcgttt ttcaatttca
     2161 gcgtttcctt ataagccgta atcgcgtctt cgtaacgatt gcggttgtag taggcgacgc
     2221 ccaaatcaaa ccacgcttcg gtaaatttcg gattgatccg cacggcttcc ttgtaggcgt
     2281 taatcgcttc cttgtccttg tcgagccggt cgtaggtttc gccctggtaa taatacgctt
     2341 cggcgtaatc gggtttcgcc ttgatagcgc cggaaaatgc cgccagagct tccgtgtttt
     2401 tattttgttt gtaataaagc aagcctttga aatattgcgt ttccgggtcg tttgcgccgt
     2461 ttacggcggt ggctttcgac aaataatttt ccgccagcgc gacctcgcct ttctgcaaat
     2521 ataaaatgcc gagcggcgcg tgcagctcgc tcaattccgg gctcaattgc agggcttttt
     2581 cataattggc gatggctttt tcgttttgat tcgaatcgtc gtagatttcg ccgaggctgg
     2641 cgaaagccgc cgcgttttta tcgtttaatt taacggcttc ctcgtagaaa accagcgacg
     2701 cctgcggatt gactttcgcg gaagtctcgt cgcctttcgc cgtcagagcc tcgctcaagc
     2761 cgagcctggc gttttcattt ttcggattca gcgccagcgc ctgccggaaa taatcgatag
     2821 ccgaatcaat ctggtttctt tccagataaa cttccgccgc gccggtcaaa gcggtcgaag
     2881 ctttttcctt ctgcgcggtt gttttgacct gcgccgtagt tttcgcaacg gttttcgggt
     2941 cgacttttac cttcgttcgc tgaggtttcg ttttgttgac ggcgacaatc tgcgctttaa
     3001 ccttccggcg cgattcgttg cgcgcgacag cgtttcgctt gacgctcgta ttacgaaaag
     3061 ctgattttgt ctgcgcggtt ttctttccct gccggaaaac gaaaacgctg gagccgccgg
     3121 aaatgtcttc ggacgtcacg atttcctgcg ctccgaccgg aatgctctga aaaattaatg
     3181 tccaaaggac aaagattgtt aaagctatct ttcgccattg cattttgttg ttcattttat
     3241 acctcataaa ccttgataaa ttaccagata ttttactctg cttttaattt caagcaagct
     3301 aatattgcct ttaatcaaat aagacgctcg gcggggagtt ttttgccgct aagaacaggg
     3361 aagttaggga aattaaggaa gataaggaaa ttaggacgat aggaaaatga ctaaagaaga
     3421 gccgcgcgag gctcccgggt ttcgcataat ttccataatt tccataattt ccttgatttc
     3481 ctatctcacg aaatatgcta aactgcaaaa tcgttaatat ctttcgtcaa tttttatgca
     3541 ggaaaaagta gcaatcgtaa cgggcgcgag ctcgggcatc gggcgggcaa cggctttgct
     3601 ttttgccgaa aagggaatta aagtcgtcgg cgtcgggcgc agcgaaaaag aattaaacgc
     3661 gcttcgtcag caggcaaaag aaaaaaacgg catcgtcaaa actcatctcg ccgacctgct
     3721 ggaaaacaaa caggtcgaaa gagtcatcgc cgaaaccgcg gaaaccttag gcggaatcga
     3781 cattctggtc aatgcggcgg gcattatcgc caacggttca atcgagacga ccgcgctcga
     3841 agaatgggac aagatgatga acatcaacct gcgctcggtt ttttttatta tgcagaaatg
     3901 cgttccgcat ctggaaaaaa ctcgcggaaa catcgtcaat gtttcgagcg tgacgggcgt
     3961 gcgcgctttt ccgaacgttt tggcttactg cgtttccaaa gccgccgttg accagttgac
     4021 gcgctgcagc gcgctggagt tagcgccgaa aggcgttcgc gtcaatgccg tcaatccggg
     4081 agtcgtggtg acgaatatac acaagcgcgg cggaatggac gaggaaaaat acgagcagtt
     4141 tctcgaacat tccaaaacca cacatccatt gggtcgcgtc ggcaatccga gcgaagttgc
     4201 cgagttgatt tattttctcg cttcggaaaa cgcttcgtgg ataacgggcg caacctatga
     4261 aatagacggc gggcgagcgc agacgtgcgc cagatgaaag ttgcagaaag ttgcgggaag
     4321 ttgcggatag ttgcagatag ttgcgggaag ttcaggacag tttctagttt gagcgtttac
     4381 gaaggcattt aactttctgc aactttccgg cactttccgc aacttcctgt caactcatct
     4441 gcaacttccc gtcaactttc cgtcaactat aaagaagaat gaaagtccaa ttgctaccga
     4501 caacttttga ccgaagcggg cgcgcgaccg ctcagcagca tctctgctgt tttgtcatcg
     4561 acgaccgcgt ggcgattgat gccggaagcc tcgcgatggc gaccacgccc gagcaaaaac
     4621 aaaatatccg cgacgtcgtt ctgactcacg cgcatctcga ccacatcgcc ggactgccgc
     4681 tttttatcga cgatttgttt gccacgctgg aaaatccggt gcgtgttttt gccgcgccgg
     4741 aagtaatcga aattctggag cgggatattt tcaactggga cgtttacccg cgtttttccg
     4801 agctgcaaaa caaattcggc gaagttctgc aatacgaggc ttttacggac ggaaaagaat
     4861 tctcggcggc gcatctgcgc atcaaagccg tcaaggtcaa tcacaaagtt ccgacggtcg
     4921 gttttatcat ttgcgacggc aactcgaaaa tcgccgtcag cggcgatacg gcgcagatgg
     4981 acgtcttctg gcaggtttta aatgaggaag aaaatttgag cgctctgctg attgaatgcg
     5041 cttttccgaa tgagctggaa gatttagcca acgcttcgca tcattt
//
LOCUS       DASWNE010000583         1723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_196876, whole genome shotgun sequence.
ACCESSION   DASWNE010000583 DASWNE010000000
VERSION     DASWNE010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196876"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..1114)
                     /locus_tag="VGB68_11500"
     CDS             complement(89..1114)
                     /locus_tag="VGB68_11500"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0049906.1"
                     /translation="MSVLDKVRRWIDGDTAETVLEEAARDAQVKPRSAGEEFIVKLAR
                     AVEEVMQREMTPLPQGTVLIPSEYIIFLSEEDDKDWTGVRRRGLEQGLYHILAERAKE
                     ISGKKKLETQSFAVELRVDGTLEKGDVRVQHSWDDTSSNKTSILSRPKPQLPQQPTQP
                     LKPANQVTPIPSFTPPSQTATSPNNPPPTVAQLNVQPHIPPQQQTPQAPYQPPLAQQQ
                     QTPPFEAGDSEEMTHVKKRTTELYKLEIWRGGVRQNVVPIFQNEISIGRGSKSKPVDI
                     PLAGDPEISRRHVTLICNEQGYFLISEGRNLAMVNNYEVPMGQRVTVAPGIPIAVCSY
                     LLRIQPK"
     gene            complement(1282..>1723)
                     /locus_tag="VGB68_11505"
     CDS             complement(1282..>1723)
                     /locus_tag="VGB68_11505"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEY0049907.1"
                     /translation="PPAQKPPPPKNEEFLKTYIEEEAEEAEPVRKKSFLRRAGVLLLF
                     LAVFAGALFVTQSYLRTGRIFPEIKNPFVSQQGIAINDVNLRTEANADSQKIGLVPKS
                     SRVRIVNSKDNWYEVDVLEYGRPKENAADADHGWVNRRYIDIQE"
BASE COUNT          346 a          465 c          436 g          476 t
ORIGIN      
        1 caatttctaa atttgatttt cagattcgag attgctgaaa agaaattctc tccgtcaacg
       61 cgtcgccctt tttccgtgtc tctcctactt atttgggctg gattctcaat aaatagctgc
      121 acacggcaat cggaataccg ggcgctaccg tcactctctg tcccatcgga acttcgtaat
      181 tgttgaccat tgccagattg cgtccttcgc ttatcaggaa atatccctgc tcgttgcaga
      241 tgagcgtgac gtgacggcgg ctgatttccg ggtcgccggc gagcggtatg tcgacgggct
      301 ttgatttcga gccgcgcccg atgctgattt cgttttgaaa aatcggcacg acattttgac
      361 ggacgccgcc gcgccagatt tccagcttgt agagctcggt cgtgcgcttt ttaacgtgcg
      421 tcatttcttc cgaatctccg gcttcaaaag gcggcgtttg ctgctgctgc gccaagggcg
      481 gctgataggg agcctgcggc gtttgctgct gcggcggaat atgcggctgg acgtttagct
      541 gcgcgacggt cggcggcgga ttgttcggcg acgttgcggt ttggctcggc ggcgtgaaac
      601 tcggaatcgg cgtcacttga ttcgccggct tcagcggctg cgtcggctgt tgcggcaatt
      661 gcggtttcgg gcgcgacagg atgctcgttt tgttgctcga agtatcgtcc cagctgtgct
      721 ggacgcggac atcgcctttt tccagcgttc cgtccacgcg aagctcgacg gcgaaagact
      781 gcgtttccag ctttttcttc ccggaaattt ctttcgcgcg ttccgccagg atgtgataaa
      841 gtccctgttc cagaccgcgc cgcctgacgc ccgtccaatc tttatcgtct tcctcgctga
      901 gaaaaataat atattcgctc ggaatcaaga ccgtgccctg cggcaatggc gtcatttcgc
      961 gctgcatcac ttcttcgacg gcgcgggcga gcttgacaat aaattcttcg cccgcgctgc
     1021 gcggcttgac ctgcgcgtcg cgcgcggctt cttccagaac ggtttccgcc gtgtcgccgt
     1081 caatccatcg tcttacttta tccaaaacac tcataatatt ttagatttta aattttagat
     1141 tttaggctgg acaggaattc tgcttccagt aaaggttccg tttcagaaat aaaaacgtta
     1201 agagaaaaat ttcggcgcga ctattctgaa aaaaaatcct tatccaatca aaaatctaaa
     1261 aatcgaaaat cgtcaatccc cttactcctg aatatcaata tatcgacggt tcacccagcc
     1321 gtggtcggcg tccgccgcgt tttccttcgg gcgaccgtat tccagcacat caacttcata
     1381 ccagttatct ttggaattta caatccgcac gcgtgaactt ttcggaacga gcccgatttt
     1441 ttgactgtcg gcgttggctt ccgttcgtaa atttacgtcg ttaatggcga tgccctgctg
     1501 gctaacgaaa ggatttttga tttcagggaa aattctgccg gttcgcagat aactctgcgt
     1561 gacgaacagc gcgcccgcga aaaccgccag aaataacaaa agcacaccgg cgcggcgcag
     1621 gaagcttttt tttctgaccg gctcggcttc ttctgcttct tcttcaatgt aagtctttaa
     1681 aaactcttcg tttttcggcg gcggcggttt ttgcgccggc ggc
//
LOCUS       DASWNE010000584         1630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_230847, whole genome shotgun sequence.
ACCESSION   DASWNE010000584 DASWNE010000000
VERSION     DASWNE010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230847"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VGB68_11510"
     CDS             complement(<1..204)
                     /locus_tag="VGB68_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049908.1"
                     /translation="MKRFYKNFVSRLMFFAALLPAVGCLPSAPETKHGSRTITVYGFS
                     IMKEALEKEIYPAFAAKWKREHGE"
     gene            complement(234..893)
                     /locus_tag="VGB68_11515"
     CDS             complement(234..893)
                     /locus_tag="VGB68_11515"
                     /inference="COORDINATES: protein motif:HMM:NF022142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HEY0049909.1"
                     /translation="MKKIIFALFLCGFCSVFAVAQTVADEEDFQSWNDLQLTVPLTKH
                     FDFFTQVTMRFGKNVTRLNDGRYAVGFLWKPTKSLSVSPFYWHIGARNSAGRFRTEHR
                     LNLRAIYRFPFKKFGLLHRSWYEYRLRRPLNSWRYRPSLTFEKDIPKKFSPAARLFVT
                     EEIFYDSLLKKFSRNRFTVGITKTLTKNLSLDAYFMRQNDGFSRPGDLNVIGTSWKVR
                     L"
     gene            complement(890..1117)
                     /locus_tag="VGB68_11520"
     CDS             complement(890..1117)
                     /locus_tag="VGB68_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049910.1"
                     /translation="MNRRYFWQVPEYIKFRIVFEDAETILGATANNEYRLFRKDTSQI
                     VFSICEALDVCFASLMMQESESLSELEAGVV"
     regulatory      complement(1230..1367)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
BASE COUNT          425 a          390 c          391 g          424 t
ORIGIN      
        1 ctcgccgtgc tcgcgtttcc acttggcggc aaacgccgga tagatttctt tttccagcgc
       61 ttctttcata atcgaaaagc cgtagacggt aatcgtccga ctgccgtgtt tcgtctccgg
      121 cgcgctcggc aggcagccga ccgccggaag aagcgccgcg aaaaacatta accgcgaaac
      181 aaaattttta tagaaacgtt tcatatttca aatctccgag cccgcgcgcg gatttacagg
      241 cgcactttcc agctcgtgcc gatgacgttc aaatcgccgg ggcgagagaa tccgtcgttt
      301 tgccgcataa agtaagcgtc cagagataag tttttcgtta aagtctttgt aattccgacc
      361 gtgaaacgat tgcgcgaaaa tttcttcagg agcgaatcat aaaagatttc ttcggtcacg
      421 aaaagcctgg cggcgggact gaattttttc ggaatgtctt tttcaaacgt cagcgacggt
      481 cgatagcgcc acgaattcag cggtcggcgc aagcgatatt cgtaccagct gcgatgaaga
      541 agcccgaatt ttttgaacgg aaaacgataa attgcgcgca gattcagacg atgttccgtg
      601 cgaaatctgc cggcgctgtt gcgcgcgccg atgtgccagt aaaaaggcga gaccgacaag
      661 gatttagtcg gtttccagag gaaaccgacc gcgtatctgc cgtcgttcag acgcgtcaca
      721 ttcttgccga aacgcattgt gacctgcgtg aaaaaatcga aatgcttcgt cagcggaacg
      781 gtcagctgca aatcgttcca ggactgaaag tcttcctcgt cggcaaccgt ctgcgcgaca
      841 gcgaaaaccg aacaaaagcc gcataaaaac aaggcgaaaa tgattttttt catacgacgc
      901 ccgcctcaag ctcagataaa gattcggatt cctgcatcat cagcgaagcg aagcatacgt
      961 ccagtgcctc gcagatgctg aaaactatct ggctggtgtc ttttctgaac aggcgatatt
     1021 cgttgtttgc ggtcgcgcct aaaattgttt cggcatcttc aaaaacgatg cgaaatttga
     1081 tgtactcggg aacttgccaa aaatatctgc gattcatatc aattaaacct ccgttttttt
     1141 tatcagcgga agctacgccc gattcggcgg atttcagata cagaaaaagg tatgaagcac
     1201 acgcataata ccttctgcaa aatcggtcgt gtcctccttc gttacgccta cgaggttagc
     1261 tgacgggctg gaagcgaaga agtcttcttc tggttataaa accggatacg ccccgaaaag
     1321 ttggttcccc cgtgttattt tttagataac ttcggcgact ttttaattgt gtcggttaga
     1381 ttagcaaatc aagatggtat ttggcaatac tttttttcga gttttataaa aaaaatagtt
     1441 aagtggtaaa cattaaacgc taaacggcaa gttttacttc gccattgtaa ctaatcgctt
     1501 accgtctatc gtttaccgct ttatcgaagc ggcaatgcgg gcggcgattt cggcgttatt
     1561 ttccagcaga gcgacgtttg ccgataaggt ttttccggcg cttttttcgg acatttgcgc
     1621 gagcagaaac
//
LOCUS       DASWNE010000585         5097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_55422, whole genome shotgun sequence.
ACCESSION   DASWNE010000585 DASWNE010000000
VERSION     DASWNE010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5097
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55422"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1227
                     /locus_tag="VGB68_11525"
     CDS             <1..1227
                     /locus_tag="VGB68_11525"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0049911.1"
                     /translation="TESAEVATAGAATAETASGAAAETVSSDASATAAAPAPETSQPV
                     DTEFLKSELAKLNAELKEVQGETPLMQPVVNGQTIAEVISGWTGIPVGKMVADEILAV
                     LKLAETLKQRVVGQDHALNAISERIKTSRAGLTDPKRPIGVFLLVGTSGVGKTETALA
                     LADTLYGGERNLISINMSEYQEAHTVSSLKGSPPGYVGYGEGGVLTEAVRRKPYSVVL
                     LDEVEKAHPDVMELFYQVFDKGQLEDGEGREIDFKNTVILLTSNVATDTIMTLCADPD
                     TKPEPEGLLEAIRPELSKAFKPALLGRMTVAPYYPISDDVLRLIVKLQLTKIAQRIAA
                     NHKAQFSYDDSVIETVARRCTDVDSGARNVHNILNGTLLPEMSGEFLSRMATGEGLTR
                     VHVSADESGKFIYEVV"
     gene            1240..3390
                     /gene="tssI"
                     /locus_tag="VGB68_11530"
     CDS             1240..3390
                     /gene="tssI"
                     /locus_tag="VGB68_11530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HEY0049912.1"
                     /translation="MATTQDGRLLSISTPLGKDFLLLKRMKATEGLSELFQIDVELLH
                     EETSEGFEATPVDVQRILGQSVSIAVTQRDGNTRAFNGIVNRFSQGTRDDRFSYYYAT
                     IVPHVWILTQNVQSRIFQHVSVPDILKKVFAGFEIVPEIQGTFHKRNYCVQYRESDFA
                     FASRLMEEEGIYYYFEHAGGTHKMIIANTPQSHRACPGKSSVPFFIDVTRGQEDWVSS
                     IATWRQDYQLQTGRVAFWDHNFELPGKHLEVEKKSRFNVGGNQELEIYEFPGGYARKY
                     DGIDRTGGVRASDLQHIFEDNQRTAQIRIEELDSQHLTANAIADCASLSAGYRFTLFN
                     HPTRKNNGDYVLTAVTHEAEQSPNYVANEEIAGAYGNSFSCIAQGAGAPPFRPPQKTP
                     KPMIQGSQTATVVGPPGEEIFTDKYGRVKVQFHWDRHGRVDADSSCWVRVAQGWAGNK
                     WGTMFIPRIGMEAVVHFLEGDPDQPIVTGCVYNPEAMPPYKLPDEKTKMTIKSNSSKG
                     GAGFNEFRFEDKKGEEQIFMHGEKNLDIRIKNDALETINRDRHLTVERDQLEKVKKDK
                     HLIIGGNQNEKIGGGLSIKVGTSEQIKTGTKFAVDAGTEIHLKAGVNATIEAGTSLTL
                     KVGGNFINISPAGIAIKGTMVLINSGGAAGSGSGSSPETPKEPKPADTANPGERIKSP
                     KPPPPMKPTSYSQQAQGLINAAQSGTPFCAICGM"
     gene            3409..3948
                     /locus_tag="VGB68_11535"
     CDS             3409..3948
                     /locus_tag="VGB68_11535"
                     /inference="COORDINATES: protein motif:HMM:NF024894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4123 domain-containing protein"
                     /protein_id="HEY0049913.1"
                     /translation="MNRRISMEQEQQLNQHLFSRVTRVYAVLDGASTPGLPTRLYELQ
                     PPHFCLFTGDLEPDMLEVAPYLVRLLPKTPFTDWILRDGWGKHWGIFLHSREPIEEMR
                     AHFRSLVTVYDEKGTPMIFRYYDPRVMGKFLQTCKPDELKTFFGNVEAYFAETKDAGN
                     LIRFENGEDGLIETVLPVK"
     gene            3964..4467
                     /locus_tag="VGB68_11540"
     CDS             3964..4467
                     /locus_tag="VGB68_11540"
                     /inference="COORDINATES: protein motif:HMM:NF017315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HEY0049914.1"
                     /translation="MPTGPAARTTDNVLHPLPPVLTIGPGSLNVLIGFLPAWRGIPLA
                     AVAALQAAKTAADTAVKTAEAATVAAAGTPGAPAAYAAEQAAKGAALASMSSAITGAA
                     AAGADIHTCVTPSPVPPHGPGVVIDGSATVQINFLPACRLGDTILEALGPPNKIVRGE
                     TTVIIGG"
     gene            4523..4966
                     /locus_tag="VGB68_11545"
     CDS             4523..4966
                     /locus_tag="VGB68_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049915.1"
                     /translation="MLTIRQEQMDSLIMGSDEEFVEFLVAHVKEEYPGIETENDDETL
                     REMVRGGIERAGSHELTTAEDITAFISIMFEIAPNFDEQPEIKAVLDDEKTPPEERLE
                     KLWTPAVPEEAWEEAEKNYDDNAWFAYRVENEATGGAEETGSTDN"
BASE COUNT         1272 a         1356 c         1488 g          981 t
ORIGIN      
        1 acggaatccg ccgaagttgc gacggcaggc gcggcgacgg ctgagacagc ttcgggcgca
       61 gcagcagaaa ctgtttcgag cgacgcatcg gcaacagccg ccgcgcccgc gccggaaacg
      121 agccagccgg tcgataccga atttttaaaa tccgagctgg ctaaattaaa cgcggaactc
      181 aaagaagttc agggcgagac gcctctgatg cagccggtcg tcaacggtca gaccatcgcc
      241 gaagtcattt ccggctggac gggaattccg gtcggcaaaa tggtcgccga cgagattctg
      301 gcggttttga agctggcgga aacgctcaag caaagagtcg tcgggcaaga ccacgccctg
      361 aacgcgattt ccgaacgcat taaaacttcg cgcgccggtt tgaccgaccc gaaacgtccg
      421 attggcgttt tccttttagt cgggacttcc ggcgtcggta aaaccgaaac cgccctggcg
      481 ttagccgaca cgctttacgg cggcgagcgc aacctgattt cgataaatat gagcgaatac
      541 caggaagcgc acacggtttc cagtttgaaa ggctcgccgc cgggatatgt cggctacggc
      601 gaaggcggcg ttttgaccga agccgtgcgt agaaaaccgt attcggtggt tttgctcgac
      661 gaagtcgaaa aagcgcaccc ggacgtgatg gagcttttct accaggtttt cgacaaaggt
      721 caattggaag acggcgaagg acgcgagatt gatttcaaaa acacggtgat tctgctgacg
      781 tcaaacgtgg cgaccgacac gattatgaca ctgtgcgccg acccggacac caagcccgag
      841 ccggaaggct tgctcgaagc gattcgaccg gaactgtcta aagcctttaa acccgcgctt
      901 ttaggacgta tgacggtcgc gccgtattac ccgatttccg acgacgttct gcggctcatc
      961 gtcaaattgc agctgacgaa aatcgcgcag cgcatcgcgg cgaatcacaa agcgcaattt
     1021 tcctatgatg attccgtcat agaaactgtg gcgcggcgct gcaccgatgt cgactcgggc
     1081 gcgcgcaacg ttcataacat tctgaacggg acgcttttgc cggaaatgtc gggcgagttt
     1141 ttatccagaa tggcgacggg cgaaggcttg acccgcgttc acgtttcggc ggacgagagc
     1201 gggaaattta tttacgaagt ggtttaaagt tattaattta tggcaacgac acaggacggc
     1261 agattattga gcatttcgac gcctttgggc aaagactttc ttcttttaaa aagaatgaaa
     1321 gcgaccgaag gactgtccga gctttttcag attgacgtcg agctgcttca cgaggaaacg
     1381 agcgaaggat tcgaagcgac gccggtcgac gtgcagagaa ttctcggtca atccgtgtcg
     1441 attgccgtaa ctcagagaga tggaaatacg cgcgcgttta acggaatcgt caaccggttt
     1501 tcgcaaggca cgcgcgatga tcgcttctct tattactacg cgacgatcgt gccgcacgtc
     1561 tggattttga cgcagaacgt gcaaagccgg attttccagc acgtgagcgt gccggatatt
     1621 ctcaaaaaag tctttgcggg ctttgagata gttcccgaaa ttcaaggcac gtttcacaaa
     1681 cgaaactatt gcgtgcagta tcgcgaatcg gatttcgctt tcgcctcgcg gctgatggaa
     1741 gaagaaggaa tttactatta cttcgagcac gcgggcggaa cgcacaagat gattatcgcc
     1801 aatacgccgc agtcgcatcg cgcctgcccg ggcaaaagct cggttccttt ttttatagac
     1861 gtcacgcgcg ggcaggaaga ttgggtcagc tcgattgcca cgtggcggca ggattatcaa
     1921 ctgcaaaccg gaagagtcgc gttctgggac cataatttcg agcttccggg caaacatctg
     1981 gaagtggaaa aaaagagtcg ctttaacgtc ggcggcaatc aggaactgga aatctacgaa
     2041 tttcccggcg gctacgcgcg aaaatacgac ggaattgacc gcacgggcgg cgtcagggca
     2101 agcgacttgc agcatatttt tgaagacaat caaagaaccg cgcagattcg catcgaagag
     2161 ctggattcgc agcatctaac cgccaacgcg attgccgact gcgcttcgct gagcgccggt
     2221 tatcgcttta cgctttttaa tcacccgacg cgaaaaaaca acggcgatta cgttctgacc
     2281 gccgtcacgc acgaagccga acaatcgccg aactacgtcg ccaacgaaga aatcgccggc
     2341 gcttacggca acagtttcag ttgtatcgcg cagggcgcgg gcgcgccgcc ctttcgcccg
     2401 ccgcagaaaa cgccgaagcc gatgattcag ggcagccaga cggcgacggt cgtcggtccg
     2461 ccgggagaag aaatttttac cgacaaatac gggcgcgtaa aagttcagtt tcattgggac
     2521 cggcacggca gagtcgatgc cgacagctcc tgctgggtgc gcgtcgcaca gggctgggcg
     2581 ggcaataaat ggggaacgat gtttattccg cgcatcggaa tggaagcggt cgtgcatttt
     2641 ctggaaggcg accccgacca gccgattgtc accggctgcg tttataaccc ggaagcgatg
     2701 ccgccttata aactgccgga cgaaaaaacg aaaatgacga ttaagtccaa ttccagcaaa
     2761 ggcggcgcgg gctttaacga atttcgtttt gaggacaaaa aaggcgaaga gcaaatcttt
     2821 atgcacggcg agaaaaatct cgacatccgc atcaagaacg acgcgctgga aaccatcaac
     2881 cgcgaccgcc atctgacggt cgagcgcgac cagcttgaga aagtcaaaaa agacaagcat
     2941 ttaatcatcg gcggcaatca gaacgagaaa atcggcggcg ggctctcgat aaaggtcgga
     3001 acgagcgagc agattaagac cggaacgaaa ttcgcggtcg atgccggaac ggaaattcat
     3061 ttaaaggcgg gcgtcaacgc cactatcgaa gccggaacgt ctttgacctt aaaagtcggc
     3121 ggcaatttta tcaacatcag cccggcgggc atcgccatca agggaacgat ggttttgata
     3181 aacagcggcg gcgcagccgg ttcgggttcc ggctcaagcc cggaaacgcc gaaagagccg
     3241 aaaccggcgg acacggcaaa cccgggcgag cgcataaaat cgccgaaacc gccgccgccg
     3301 atgaagccga cctcttacag ccagcaggcg cagggcttga taaacgccgc gcagagcgga
     3361 acgccgtttt gcgcgatttg cggaatgtag acaaattttt ttttgcgggt gaaccgaaga
     3421 atcagcatgg agcaggagca gcagcttaat caacatttat tttcgcgcgt gacgagagtt
     3481 tacgccgttc tggacggagc ttcgacgccc ggtctgccaa cgcggcttta cgagctgcag
     3541 ccgccgcatt tctgcctgtt taccggcgat ttggagcctg acatgctgga agttgcgccg
     3601 tatctggtca ggcttttgcc gaagacgccg tttaccgact ggattttgcg cgacggttgg
     3661 ggaaaacact ggggaatctt tctgcacagc cgcgagccga tagaggaaat gcgggcgcat
     3721 tttcgctctc tcgtcaccgt ctatgacgaa aagggaacgc cgatgatttt ccgctattac
     3781 gacccgcgcg tgatgggcaa atttttgcag acgtgcaagc cggacgagct gaaaactttt
     3841 ttcggcaacg tcgaagcgta ttttgccgaa acgaaagacg ccggtaattt aatccgtttt
     3901 gaaaacggcg aagacggttt aatcgaaacc gtcctgccgg ttaaataatc aggagaaaaa
     3961 actatgccga ccggaccagc cgccagaacc accgataacg ttcttcatcc tttaccgccc
     4021 gttttaacca tcggaccggg aagcctgaac gtcctgatcg gctttcttcc ggcgtggcgc
     4081 ggaattccgc tcgccgccgt cgccgcgctg caagcggcga aaaccgccgc cgatacggct
     4141 gtcaaaacgg cggaagccgc cactgtagcc gccgccggaa cgcccggcgc gcccgccgct
     4201 tatgccgccg agcaggcggc gaagggcgcg gctctggctt cgatgtcgag cgcgattacg
     4261 ggcgcggctg ccgcgggcgc tgatattcac acctgcgtga cgccttcgcc ggttccgccg
     4321 cacggtcccg gcgtggtgat tgacggctcg gcaaccgtcc agattaattt tctgccggct
     4381 tgtcgcctgg gcgacacgat tctggaagcg ctcggtccgc cgaataaaat cgtcaggggc
     4441 gaaacgactg tcattatcgg agggtaaacg cgagattatt tatctgcggc gggcaatgaa
     4501 tttgagagga gaaaaaagtt aagtgttgac catcagacag gaacaaatgg acagcctgat
     4561 aatgggcagc gacgaggaat tcgtcgaatt tctcgtcgcg cacgtaaagg aggaatatcc
     4621 cggcatcgag accgagaacg acgacgaaac tctgcgcgag atggttcgcg gcggcatcga
     4681 gcgcgccggc agccacgagc tgacgaccgc cgaggacata acggcgttca tttcgataat
     4741 gtttgaaatc gcgccgaatt ttgacgagca gccggaaatc aaagccgtgc tggacgacga
     4801 aaaaactccg cccgaggagc gtctggaaaa attatggacg cctgccgtgc cggaagaagc
     4861 gtgggaagaa gcggaaaaaa attacgacga caacgcgtgg tttgcctatc gcgtagaaaa
     4921 cgaagcgacg ggcggcgcgg aagaaaccgg ctcgacggat aattaaaaat gcctagaatt
     4981 ccgagaattt cgacagggag attgttgagc gcggcagccc gcgccagaag cgcagccgga
     5041 gccggacgaa gcgcagccag aggcagagcc gttacgggct gcccgctggc ggctctc
//
LOCUS       DASWNE010000586         1513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_159190, whole genome shotgun sequence.
ACCESSION   DASWNE010000586 DASWNE010000000
VERSION     DASWNE010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159190"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..498)
                     /locus_tag="VGB68_11550"
     CDS             complement(124..498)
                     /locus_tag="VGB68_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049916.1"
                     /translation="MLILDAVLFEDGNGEGEATQLKRLQDNREGVRIVFQKAVAFARR
                     LSAANEISKIDILAQALEKELEAEFKKVPADLKGGFMDGKNLIITDLKELADKSQSEK
                     TLQQREAVEKTIFRFEGILAKL"
     gene            complement(559..873)
                     /locus_tag="VGB68_11555"
     CDS             complement(559..873)
                     /locus_tag="VGB68_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049917.1"
                     /translation="MSQLLRSRLVLTLSVFLLTISVIAVFAARQLQPVKDEVRVENKT
                     DSLSIESVTRISKQTTPKRFRITVKNTSGKNIVAYSFRQIDAASPKDSYQGIETNGAM
                     IG"
     gene            complement(1097..1408)
                     /locus_tag="VGB68_11560"
     CDS             complement(1097..1408)
                     /locus_tag="VGB68_11560"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY0049918.1"
                     /translation="MDNRISTLIIYLENNLDHRLSINEMAQFVRISPSRLQHVFKEET
                     GVSINQYLRKARLKKAAELLTRSFLSVKEIRCSVGIIDTIHFASDFKRTYGMTPSSYR
                     K"
BASE COUNT          405 a          306 c          334 g          468 t
ORIGIN      
        1 cagaaaggtt gttcggtctg tttgttgtag gctaaacaac cgcgaattgt tgttggtcaa
       61 ctcttacatt attcccgctt acatttcgtg tcaataactc tttgtctgac tttttgaatc
      121 gctttataat tttgcgagaa tgccttcaaa tctgaaaata gttttctcga ctgcttcgcg
      181 ttgttgtaaa gtcttttcgg attgagattt atcggcgagt tctttcaaat ccgtaataat
      241 taaatttttc ccgtccataa atccgccctt taaatcggcg ggaacttttt taaactcggc
      301 ttcgagttct ttttcaagcg cctgagccag aatatctatt ttggagattt catttgcggc
      361 ggacagccgg cgggcgaaag cgacggcttt ttgaaagaca atcctgacgc cttcgcgatt
      421 atcctgcaat cgttttagct gcgtcgcttc gccttcgccg tttccgtctt caaataaaac
      481 ggcgtcgaga ataagcaata tctcgccttc ggcaaaagaa ctcgccagcg tcgaatcggt
      541 cttgcccggc ggcagcgctc agccgatcat cgcgccgtta gtttcaattc cctgataaga
      601 gtctttggga ctcgccgcat ctatctgccg gaaagaatag gcgacgatat ttttacccga
      661 agtgtttttt acggttatgc gaaatcgctt cggggtggtt tgtttgctta tcctggttac
      721 ggattcgata gaaagagaat cggttttgtt ttcaactctt acttcatcct taacgggttg
      781 aagctgcctg gcggcaaaaa cggcaattac tgaaattgta agcagaaaaa ccgataaagt
      841 taaaacgaga cgggaacgta gaagttggct catcactaat tcctttttta ttaaagtgat
      901 atttacagct tttcagagtt tttccggctt aaacgaaaag tcgggcgcaa aactccggtt
      961 agagatttaa gcaacaaaag atttatcaag gttaatggta agtcgattga aaagcaataa
     1021 cctttttctg ctattcgata gccgattctg ctgattgctt tctctgatac gtgtcagagg
     1081 catctaaatc gaaatgttac ttgcgataag aggaaggagt catcccgtaa gttcgtttga
     1141 aatcactggc aaaatgtatc gtgtcgatga tgccgaccga gcagcgaatt tctttgacgc
     1201 ttaaaaatga gcgggtcaaa agctccgccg cttttttcag tcttgctttt cgcaggtatt
     1261 gattaattga aacgccggtt tcctctttaa aaacgtgttg cagccgggat ggagaaattc
     1321 ggacgaattg cgccatttca ttgattgaaa gccggtgatc gagattgttc tcaagataga
     1381 tgataagcgt cgagattctg ttatccattt gaatttcctt tctatatgga agaaatgaaa
     1441 atcaatcgaa aaataaatgt tttaccctaa agaaaacgaa atagccgtga aaccttgaag
     1501 agtaagtgat acg
//
LOCUS       DASWNE010000587         1899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_268078, whole genome shotgun sequence.
ACCESSION   DASWNE010000587 DASWNE010000000
VERSION     DASWNE010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268078"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="VGB68_11565"
     CDS             complement(<1..790)
                     /locus_tag="VGB68_11565"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049919.1"
                     /translation="MSKRLPNLAPPFAKIRNRLANYSKRLLQSLTKRQVLFIATVVFI
                     LVTTILIQNPLWRAQGSEQYKEGDILRESIISPADISITDTAETEKNRQMSRESVFPI
                     FTLESNRAETAVQNFRSAWENLQRHGDISDSNARSENSSNARANVKEDSKAETHWTGA
                     GGAEVGTIFAARRFSSNELEAVSRALRESADGRIYNDSDQQYFQENITLIDRQKPSSQ
                     ITERSPETSWTALSAAREKLKNRLAEIRSLSQKEADAFYIALESY"
     gene            complement(840..>1899)
                     /gene="hflX"
                     /locus_tag="VGB68_11570"
     CDS             complement(840..>1899)
                     /gene="hflX"
                     /locus_tag="VGB68_11570"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEY0049920.1"
                     /translation="AELEELALSADVVVLDKIIQRRQQIDSRTVLGKGKLEELLVRAM
                     RLGADLIVFDTELSPAQVRSLSEATDLKVIDRPQLILDIFAQRAQSREGKIQVELAQL
                     KYILPRLIAGQNSAFSRLAGGIGGRGPGETKLETDRRRVRDKINRLEKEIDHLSYQRA
                     NRRKERLKRDVPIISLVGYTNAGKSTLLNTLTQSKVYAEKKMFATLDPTSRRLRLPYE
                     QEVIINDTVGFIRDLPVALVAAFRATLEEIGDSDMLVHVVDASNPRALAQIESVDKIL
                     GDLKLNEIPQLIVLNKADLLEESDGEALKRQLSLDKQTESVAISAIKPETLPPLLEKI
                     GEVLAKDLNKVSVFGLAS"
BASE COUNT          391 a          492 c          528 g          488 t
ORIGIN      
        1 tgtaggattc gagcgcgata tagaatgcgt cggcttcttt ctgcgaaaga ctgcggattt
       61 ccgccagacg gtttttcagt ttttcgcgcg cggcggaaag cgccgtccag ctggtttcgg
      121 gcgagcgctc cgtaatctgg ctgctcggtt tctggcggtc aatcagcgtg atattttcct
      181 gaaaatactg ctggtcggaa tcgttataga tgcgaccgtc ggcgctctcg cgcaaggctc
      241 tggaaacggc ttcgagctca ttcgaactga accgccgcgc ggcaaaaata gtgccgacct
      301 ctgcgccgcc cgcgcccgtc caatgcgttt cggctttgga gtcttcctta acgttcgctc
      361 tggcgttcga ggaattctcc gaacgggcgt tggaatcgga aatatcgccg tgacgctgta
      421 gattttccca cgccgagcga aagttctgga cggcggtttc ggcgcggttc gattcgagcg
      481 tgaaaatcgg aaaaacgctt tcgcgcgaca tctgccggtt tttttcggtt tcagcggtgt
      541 cggtgatgga aatatcggcg ggcgaaataa tgctttcgcg cagaatatcg ccttctttat
      601 attgctcgct gccttgtgcg cgccataggg gattttgaat gagaatagtc gtaaccagaa
      661 tgaatacaac agttgcaata aaaagaactt gtcttttggt taaagattga agcaggcgct
      721 tcgagtagtt cgccagcctg ttgcggattt tcgcaaacgg cggcgcgaga tttggaagac
      781 gcttactcat tttagatttg gctccggatt tcgcgtttcg gtcttcgcaa aacaaagact
      841 caggacgcca gaccgaagac cgaaacttta ttcaagtcct tcgctaaaac ttctccgatt
      901 ttttccagaa gcggcggcag tgtttccggc ttgatagccg aaattgccac gctttcggtt
      961 tgtttgtcaa gcgacagctg gcgttttaag gcttcgccgt ccgattcttc aagtaaatcc
     1021 gctttgttta aaactatcaa ttgcgggatt tcgttgagct ttaagtcgcc gagaatttta
     1081 tcgaccgatt cgatttgcgc caacgcgcgc gggtttgagg cgtcgaccac atgaaccagc
     1141 atatcgctgt cgccgatttc ttcgagcgtc gcccggaagg cggcgaccag cgcgacgggc
     1201 aaatcgcgga taaacccgac cgtgtcgtta ataataactt cctgctcgta cggcaggcgc
     1261 agccggcgcg aggtcgggtc gagggtggca aacatttttt tctcggcgta aactttgctt
     1321 tgcgtcagag tgttgagcaa agtcgatttt ccggcgttcg tgtagccgac gagcgagata
     1381 atcggcacat cgcgctttaa tctttccttg cgtcgattgg cgcgctggta actcaaatgg
     1441 tcgatttctt tctccagccg gttgattttg tcgcggacgc ggcggcggtc ggtttcgagt
     1501 ttcgtttcgc cgggaccgcg tccgccgatg ccgcccgcca ggcgtgaaaa cgccgaattc
     1561 tgcccggcga taagacgcgg cagaatgtat tttaactgcg ccagctcgac ctgaattttg
     1621 ccttcgcgcg attgcgctcg ctgcgcgaaa atatcgagaa taagctgcgg gcggtcgatg
     1681 acttttaaat ccgtcgcttc cgacagggaa cgaacctgcg cgggcgaaag ctccgtgtcg
     1741 aaaacgatta aatccgcgcc gagacgcatc gcgcgaacca gcagttcttc gagctttcct
     1801 tttcctaaaa cggttctgga atcaatctgc tggcggcgtt gtatgatttt atcgagcacg
     1861 accacatcag ccgaaagcgc gagctcttca agctccgcc
//
LOCUS       DASWNE010000588         3454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_268143, whole genome shotgun sequence.
ACCESSION   DASWNE010000588 DASWNE010000000
VERSION     DASWNE010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268143"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VGB68_11575"
     CDS             complement(<1..500)
                     /locus_tag="VGB68_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049921.1"
                     /translation="MKERILLILSLILAITGLVFAQTRATKTVTNADLEKFRQKRVQN
                     EADYRANYKRLGMPSPEELEEREVERQRWLAEFSQKAEAERAESENYFLSRADELKTQ
                     INSIDAQINYLRAQFGQPSPYKGGTIVYGGGLVLGGYAYGYGGRNRGYRQNRAGVGPN
                     VQTVRN"
     gene            complement(793..3177)
                     /locus_tag="VGB68_11580"
     CDS             complement(793..3177)
                     /locus_tag="VGB68_11580"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0049922.1"
                     /translation="MKAVKILSLICFLTIGVWAQSVDYLGIEGKVTDSEGESIGDAEV
                     TVTSETYKDFRKTTRTDDQGNYRFSVNAPGIYILTASYSVNGEVKTSAPVKSSVTRGK
                     YTTGNLQITGGAAIRAEVSVIAARTTQTIDEVSKTVNIIDGREMRERADFALTETLRT
                     IPGFRVQQLGGFGRTASIKTRGLRNQDTAVLIDGIRFRDASSITGDASAFLSDFTLTS
                     VSRIEVLRGSGSSLYGTNAIGGTIDFQTPKPQPGLHGQISGAFGDLGLGRFRGNISDG
                     TRDGKFGFNLGVSRTVYAKGIDGEDDARNANFQSRIEYNPFEKTNISARFFISDAFVR
                     LNSNPDTLGALPASNAVIINANPNVNFIADANDPDNFQKSNFFSGQFAVTQIINSRLF
                     FQGSYQGLKTARKITNGVLGVGFQPFGGDETSLFEGFIQTANGRFNWTPNRFHEITFG
                     YEYEREKFGNDGFTSDGSGNFRTRARAASSTFYAQDLLSFFDNRLQIAGGFRAQFFNL
                     ENPSFSDINAPYSNLTLENPPIAYTFDGAVSYFFAKTGTKIRAHVGNGYRVPSLYERF
                     GTFYSGFSQDFVALGDPNLKPERSIAFDGGVEQNLFKRRAKLTAVYFHTKLIDTIGFG
                     NLVTNIGTTPRPFGGYINTKGGIARGAEFGAQIKATGSTDIFASYTFTNSDQREPQIA
                     GSGIIETLGIPKHQFTLTATQRFKRAWVNFDYLAAGDYLAPIFSNATFNTYVYRFGGN
                     RKADLTAGYTFPLKGERLSLRLFGTIENLFGEDYYENGFRTAGRTARGGLALSF"
     regulatory      complement(3213..3410)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT          777 a          921 c          889 g          867 t
ORIGIN      
        1 taatttctga ccgtctgcac attcggtccg acgcctgctc tattctgccg gtatcctcta
       61 ttgcggccgc cgtagccata cgcgtagccg cccaaaacca gcccgccgcc gtagacaatc
      121 gttccgcctt tatagggcga cggctgaccg aactgagcgc gcaggtaatt gatttgcgcg
      181 tcgatggaat taatttgcgt tttcaattcg tcggcgcgcg agagaaaata attctcgctt
      241 tccgcgcgct cggcttcggc tttttgcgaa aactcggcga gccatcgctg gcgctcgact
      301 tcgcgctctt cgagttcttc gggcgaaggc attccgagcc ttttataatt ggctcggtaa
      361 tcggcttcgt tttgcacgcg cttctggcga aacttttcca aatcggcgtt ggtcacggtt
      421 ttcgtcgctc tggtttgcgc aaagaccaga cccgtaatgg caagaatcaa gcttaaaatc
      481 aacaaaattc gttctttcat cgcttttacc tcttttgttc ttttgttcga ttatagcatt
      541 aatgtgaaac ggaaaagaag aaaattcagc cgccgagggc aaagagaacg ccggcggaat
      601 aaaaccgcag gtaaactcaa acaaataaaa acgaggaatg cggcgcgcgg aatgatgaat
      661 tgcaatttcc gcgttccgca ttcctcgttc gtcattttga tttatcccgc ccgtcctgtt
      721 taattattcg cgttaacctt cgtttacctg cggttccggt ctctgttttc gtgtgctccg
      781 gctgattcat ctttaaaaac tcagcgccaa tccgccgcga gccgtcctgc cggcggtgcg
      841 aaaaccgttt tcgtaataat cttcgccgaa aagattttca atcgtgccga aaagacgcaa
      901 gctcagtctc tcgcctttca gaggaaacgt gtaaccggcg gtcaaatcag ctttgcggtt
      961 gccgccgaag cggtaaacgt atgtgttaaa agtcgcgttg ctgaaaatcg gcgccagata
     1021 atcgccggcg gcgagataat cgaaattcac ccaggcgcgt ttgaatctct gcgtcgccgt
     1081 cagcgtgaac tgatgtttcg gaatacccag agtttcgatg attccgctgc ccgcaatttg
     1141 cggctcgcgc tggtcgctgt tcgtaaaagt gtaggacgcg aaaatgtcgg tcgagccggt
     1201 cgctttgatt tgcgcgccga actctgcgcc gcgcgcgatg ccgcctttcg tgttgatgta
     1261 gccgccgaaa ggtctgggcg tcgtgccgat gttcgtgacg aggtttccga aaccgatggt
     1321 gtcgattaat ttcgtgtgga aataaacggc ggtcagcttc gcgcgccgtt taaaaagatt
     1381 ctgctcgacg ccgccgtcga aagcgatact gcgttcgggt tttaaattcg ggtcgccgag
     1441 cgcgacaaag tcctgcgaaa aaccgctgta aaacgtgccg aagcgttcat acaagctcgg
     1501 aacccggtag ccgttgccga cgtgggcgcg aattttcgtt cccgttttgg cgaaaaagta
     1561 ggaaacagcg ccgtcgaaag tgtaagcaat cggcggattt tccagcgtca gattagaata
     1621 aggcgcgttg atgtcgctga aagacggatt ttccagattg aaaaactgcg cgcgaaagcc
     1681 gcccgcgatt tgcagtcgat tatcgaaaaa gctcagtaaa tcctgcgcgt aaaacgtgct
     1741 gctcgctgcc cgcgcgcgcg tccgaaaatt gccggaacca tcgctcgtaa aaccgtcgtt
     1801 gccgaatttt tcccgttcgt attcgtagcc gaaagtgatt tcatggaaac gattcggcgt
     1861 ccagttaaaa cgcccgttcg ccgtctgaat aaagccttcg aaaagactgg tctcgtcgcc
     1921 gccgaacggc tgaaagccga cgcccagaac gccgttcgta attttccgcg ccgttttcaa
     1981 gccctgatac gagccttgaa aaaacaatct gctgttgatg atttgcgtga cggcgaattg
     2041 cccgctgaaa aaattcgatt tttgaaaatt gtccgggtcg ttcgcgtccg caatgaaatt
     2101 gacgttcgga ttcgcgttaa tgattacggc attcgaagcg ggcagagcgc cgagcgtgtc
     2161 gggattcgaa ttcaagcgga cgaaagcgtc cgagatgaaa aatctggcgg aaatgtttgt
     2221 tttctcgaac gggttgtatt cgattcggct ctggaaattc gcgttgcgcg cgtcgtcttc
     2281 gccgtcgatt cctttcgcgt aaaccgtgcg cgagacgccg agattaaaac cgaattttcc
     2341 gtctcgcgtg ccgtcggaaa tgttgccgcg aaagcgcccc aaacccaaat cgccgaacgc
     2401 gccggagatt tgcccgtgca agcccggctg cggcttcggc gtttgaaaat caatcgtccc
     2461 gccgatggcg ttcgtgccgt agagcgagct gcccgaaccg cgcagaacct cgatgcgcga
     2521 aacgctcgtc aaagtaaaat ccgacaaaaa agccgaagcg tcgcccgtga ttgacgaggc
     2581 gtcgcgaaaa cgaattccgt caatcaaaac cgccgtgtcc tgattgcgca agccccgcgt
     2641 ttttatcgaa gccgtacgcc cgaaaccgcc taattgctgg acgcgaaaac cgggaatcgt
     2701 ccgcaaagtt tcggtcaaag cgaaatcggc gcgctcgcgc atttcccgcc cgtcgatgat
     2761 attgacggtt ttggaaactt cgtcaatcgt ctgcgtcgtt cgcgcagcga taacggaaac
     2821 ctctgcgcgg atagccgcgc cgccggttat ctgcaaattg ccggtcgtat atttgccccg
     2881 cgttacggat gatttgacgg gcgcggaagt tttgacttca ccgttgaccg aatacgacgc
     2941 ggttagaata taaattcccg gcgcgtttac tgaaaacctg taatttccct ggtcgtcggt
     3001 gcgggtcgtt tttctgaagt ctttgtaagt ttcggacgtg accgtcactt ccgcgtcgcc
     3061 gatggattcg ccttcggaat cggtgacttt tccctcaatg ccgagataat cgacgctctg
     3121 cgcccagacg ccgattgtca aaaaacaaat taaactcaaa atttttactg ctttcatttc
     3181 ttctcctttt ccgcccagcg cggaacgtgg tttttgtttc caaacaaacg gcaaggtttc
     3241 ctgacttaca gcttatattt ccaacggcgc gccttcccat ttttatataa aaacagtggc
     3301 tttcgagcgc cgcgtcctgc tgattacagt tgcgtgggca gtcgcggact tgcaccgcgt
     3361 tcccttgcac cgcttattca attacgaatt acggaattac gaattacgaa ttaatgaacg
     3421 ctttccgaca ttcgtaattc gtaattcgta attc
//
LOCUS       DASWNE010000589         2635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_159236, whole genome shotgun sequence.
ACCESSION   DASWNE010000589 DASWNE010000000
VERSION     DASWNE010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159236"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1666
                     /locus_tag="VGB68_11585"
     CDS             <1..1666
                     /locus_tag="VGB68_11585"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0049923.1"
                     /translation="PRPFSIESPFETTLSNGLKVVVVEQRRLPLVSFRLAFRSGEIND
                     PKDSIGLTSATMHLLSQGTETRSSREIAEAVERLGASLSASSSSDNTIVSASVLTVYR
                     AEILKLMAEMILTPTFPENEIALYKQNTIENLKFQRSQPGFLADEQMSRILYGAHPYS
                     IVAPGEADIEKLSREHLVNHHRRTFVPNNATMIVVGDVDKDALVGELETLFGDWQKGS
                     VESSEFDALPQRSETTLTIVDRKGSAQSNIVLSNVAIKRNHPDFFPLLVMNQILGAGA
                     SSRLFMNLREEKGYTYGAYSSLDARRLAGAFEATAEVRTPVTGDSLKEFFYELKRIRD
                     EDVSIEELQDAKNFLTGVFPIRAETQEGLTNLIVQQQLYDLPADYLQTYRENVNAVTS
                     EEIRRVANQYITPDKIAIVIVGDAEEVLPQAESYSQKIEIFDTNNNMQDISNYGKPAE
                     DATVNVTGNWSLVLEAMGQQIPVTLTLAQTDGTVSGSMQSMFGNGEIAGGKVSGNKFA
                     ATAQIEMQGQSLELSIDGAVEDSRMEGSITAPMIPTPVAFSGTKGD"
     gene            complement(1842..2279)
                     /locus_tag="VGB68_11590"
     CDS             complement(1842..2279)
                     /locus_tag="VGB68_11590"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0049924.1"
                     /translation="MSENAATAEQKMPMPKHGQVCWTEIATSNVENCKTFYAQLFGWN
                     LKKSEAAGDDMEYYEFSNEADVCPSGGLYQMGKEYGDAPPHWMSYVSVDDVDEAASKV
                     WELGGKVCVPPTDIPNVGRFCVVNDPTGATFSMITLKAWDGVS"
BASE COUNT          626 a          668 c          704 g          637 t
ORIGIN      
        1 cccgcgcccg ttttccattg aaagcccttt tgagacgact ttgtcgaacg gcttgaaagt
       61 cgtcgtcgtc gagcagcgac gactgccgct cgtcagtttt cggctcgctt ttcgaagcgg
      121 tgaaataaac gacccgaaag attccatcgg tctgacttcg gcgacgatgc acctgctgag
      181 ccagggaacg gaaacgcgtt cgagcaggga aatcgccgaa gccgtcgagc ggctcggcgc
      241 gtcgctttcg gcgtcgagct cctccgataa cacaatcgtt tcggcttccg ttttgaccgt
      301 ttatcgcgcg gagattttga aattgatggc ggagatgatt ttgacgccga cgtttcccga
      361 aaacgaaatc gcgctctata aacaaaacac gattgagaat ttgaagtttc aacgctcgca
      421 gccgggcttt ctcgccgacg agcaaatgtc gcggattctt tacggcgcgc acccgtattc
      481 aatcgtcgcg ccgggcgaag cggacatcga aaagctttcg cgcgaacatc tggtcaatca
      541 tcaccggcgg acttttgtgc cgaacaacgc gacgatgatt gtcgtcggcg atgtcgataa
      601 agacgcgctt gtcggcgagc tggaaacgct tttcggcgat tggcaaaagg gaagtgtcga
      661 gtcttccgag tttgacgctc tgccgcagag aagcgagacg actctgacga ttgttgaccg
      721 gaaaggctcg gcgcaatcga acatcgtgct ttccaatgtg gctataaagc gtaatcatcc
      781 tgattttttt ccgcttttgg taatgaatca gattctcggc gcgggcgctt cttcgcgttt
      841 gtttatgaat ctgcgcgagg aaaaaggcta tacttacggc gcttattcga gcctcgacgc
      901 gcggcgtctg gcgggcgcgt ttgaagcgac ggcggaagtg cgcacgccgg tgacgggcga
      961 ttcgctcaaa gaatttttct atgagctgaa acggattcgc gacgaagacg tttcaatcga
     1021 agaactgcag gacgcgaaaa attttctgac gggagttttc ccgattcgcg ccgaaacgca
     1081 ggaaggcttg acgaatttaa tcgtccagca gcagctttac gatttgccgg cggattatct
     1141 gcaaacttac cgcgaaaacg tcaacgcggt cacgagcgaa gaaatccggc gcgtcgccaa
     1201 tcaatatatt acgcccgata aaatcgcgat tgtgattgtc ggcgacgcgg aagaagtttt
     1261 gccgcaagcc gaatcttatt cgcaaaaaat cgagattttc gacaccaata ataatatgca
     1321 ggacatttca aattacggta aacccgctga agacgcgacc gttaatgtga ccggaaactg
     1381 gagtctggtt ttggaagcga tgggacagca aatccccgtc actctgacgc tggcgcaaac
     1441 ggacgggacg gtttccggct cgatgcagtc gatgttcggc aacggcgaaa tcgcgggcgg
     1501 aaaagtgagc ggcaataaat tcgcggcgac ggctcaaatc gaaatgcagg gacaatcgct
     1561 cgaactcagc atcgacggcg ctgtcgaaga cagccgcatg gaaggctcga ttaccgcgcc
     1621 aatgattccg acgccggtag cctttagcgg cacgaaaggc gattaagagt tttcagcgca
     1681 gagaggcgga gaggcagaga aagaaattta cagggatgca ggggatagac ggggatagaa
     1741 attatttaac ttttttctta tccctgttca tcccctgcat ccctgtaaat ttctctttta
     1801 aaatctccgc atctccgcgg ctctgcggtt caaattttaa tctagctcac gccgtcccag
     1861 gcttttaaag taatcatcga aaaggtcgcg ccggtcgggt cgttgacgac gcagaaacgt
     1921 ccgacgtttg gaatgtcggt cggcggcacg cagacttttc cgccgagttc ccagactttc
     1981 gaggcggctt cgtcgacgtc gtcgaccgaa acgtagctca tccaatgcgg cggagcgtcg
     2041 ccgtattctt tccccatctg atacaatccg cctgacgggc agacgtctgc ttcgttagaa
     2101 aattcgtaat actccatatc gtcgcccgcg gcttcgcttt tctttaaatt ccagccgaaa
     2161 agctgcgcgt aaaaagtttt gcaattctcg acattactgg tcgcgatttc cgtccaacaa
     2221 acctgaccgt gtttcggcat cggcattttt tgttctgctg tcgctgcgtt ttctgacata
     2281 tattttctct ccttattttt ctttatttat aaatttaacc cgccgtttga tggcgggcgt
     2341 tttccgtttt ccgaacttct tccggcgaca catctttgat acgcgacgca agccggcttt
     2401 gatttccggc aaaatccgga atgccggcgc tgccgtcgcc ggaaaatata tatgccggtt
     2461 cttcggaaat tatttttccg aagaaccgtg taaaactaaa aaacgtgaga agaaaaaatg
     2521 aggatggtcg ggctgcggtg tgagattaag ctggcttcat ggagaaagct ggacggtctt
     2581 ccgcaaccca accaactggg agggattcgg ctcggtttat gcagccgaat cccaa
//
LOCUS       DASWNE010000590         1748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_159249, whole genome shotgun sequence.
ACCESSION   DASWNE010000590 DASWNE010000000
VERSION     DASWNE010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159249"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1220
                     /locus_tag="VGB68_11595"
     CDS             <1..1220
                     /locus_tag="VGB68_11595"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0049925.1"
                     /translation="KIEAGKLVLTEQVFDPRKMLRSVEEMLRMRADAKNLTLLFEVEP
                     NIPKLVKGDENKLRQVLVNLLGNAVKFTESGGVVLRCAWNEDGKAWFEVEDTGFGIAE
                     EEIGHLFEAFVQTESGRRAKEGTGLGLVISRQIVELMGGEIKVKSRLNEGTVFFFEVN
                     LQPAAESEKPAEKCKIVGLAAGEQPRRVLVADDTPENRLLLTRLLSSIGFEVREAANG
                     EEAFDLWAAWQPHLIFMDLRMPILDGYEATRKIRAGEKGRNGDDSTAENFRTKIVALT
                     ASAFESERGAAIQEGADDFLAKPFRYETIFENLTKHLGVRFLYENEEDSGAPTEIISN
                     ADFANLTPERFSVLKPELLAQFERALRSGDDLAALRVTDELRNIDPALAATIQHAVKD
                     FDFDLVLRAMGRD"
     gene            1419..>1748
                     /locus_tag="VGB68_11600"
     CDS             1419..>1748
                     /locus_tag="VGB68_11600"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0049926.1"
                     /translation="MSKGDIVIVDDNPNNLALLAGILRDNGYQARVAKSGARALEMIA
                     FQAPELVLLDIEMPEMDGYEVCRRLKANQLLQAFPVIFISALGETGDKVKGFEVGCVD
                     YVTKPFQD"
BASE COUNT          437 a          409 c          516 g          386 t
ORIGIN      
        1 ccaagataga agcgggcaag ctcgttttga ccgagcaggt tttcgacccg cgaaaaatgc
       61 tccgctcggt cgaagagatg ctgcggatgc gcgccgacgc aaaaaatctc acgcttttgt
      121 tcgaggtcga gccgaatatt ccgaaattgg tcaaaggcga cgaaaataag ctccgacagg
      181 ttctggtcaa tctgctcgga aacgccgtga aatttaccga atcgggcggc gtcgtgctgc
      241 gctgcgcctg gaatgaagac ggaaaagcgt ggtttgaagt cgaagatacg ggcttcggca
      301 tcgccgaaga ggaaatcggg catctgtttg aagctttcgt gcaaaccgaa agcgggcggc
      361 gcgctaaaga aggaaccgga ctcggactcg tcatcagccg gcaaatcgtc gagttgatgg
      421 gcggcgagat aaaagtcaaa agccgtctga acgaaggaac cgtttttttc tttgaagtaa
      481 atcttcagcc agccgccgaa agcgaaaaac cggcggaaaa atgcaaaatc gtcggattgg
      541 cggcaggcga acagccgcgg cgcgtgctgg tcgcggacga cacgccggaa aatcggctgc
      601 tgctgacgag gctcttgtcg tcaatcggtt tcgaggtgcg cgaagcggca aacggcgagg
      661 aagctttcga cctctgggcg gcgtggcagc cgcatttgat atttatggat ttgcgaatgc
      721 cgattctgga cggctacgaa gcgacccgaa aaattcgcgc cggggaaaaa gggcgaaacg
      781 gcgacgattc gacggcagaa aatttccgaa caaaaatcgt cgctctgacg gcgagcgctt
      841 tcgagagcga gcgcggcgcg gcgattcagg aaggcgcgga cgattttctg gcgaaaccgt
      901 ttcgttacga gacgattttc gagaatttga cgaagcatct cggcgttcgt tttctttatg
      961 agaatgaaga agattcgggc gcgccgaccg aaatcatctc aaacgccgat tttgccaacc
     1021 tgacgccgga gcgtttctcg gttttgaagc ccgaattgct cgcgcagttt gaacgcgctt
     1081 tgcgttcggg cgacgacctg gcggctttgc gcgtgacgga cgaactgcga aacatcgacc
     1141 cggcgctggc ggcgaccatt caacacgccg ttaaggattt cgattttgat ttggtgcttc
     1201 gcgcgatggg cagggattga aacacattga gaatgaaatc acagatggag acagataaac
     1261 gcagattcag acaggatgga caggatacac aggataattt gaaacttaaa acgtcgatgt
     1321 ttctttgtta tcctgcttat cctgtccatc ctgttaataa tccgagtttt ctcgtgttca
     1381 tctccagttt cattaattaa ttttaaggag aatttacagt gagcaaaggc gacatcgtaa
     1441 ttgttgacga caacccgaat aatctggcgc tgctggcagg aattttgcgc gacaacggtt
     1501 atcaggcacg cgttgccaaa agcggcgcgc gggcgctgga aatgattgct tttcaagcgc
     1561 ccgaactggt tttgctggac atcgagatgc ccgaaatgga cggttacgaa gtttgccgcc
     1621 gtttgaaagc caatcaatta ttgcaggcgt ttccggtgat ttttatcagc gctctgggcg
     1681 agaccggcga caaggtgaaa ggtttcgaag tcggctgtgt ggattacgtg accaagccgt
     1741 ttcaggac
//
LOCUS       DASWNE010000591         6503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_331919, whole genome shotgun sequence.
ACCESSION   DASWNE010000591 DASWNE010000000
VERSION     DASWNE010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6503
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331919"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VGB68_11605"
     CDS             <1..507
                     /locus_tag="VGB68_11605"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEY0049927.1"
                     /translation="LKEKLAAVLIQMPPQFEGSKENARNLRNFLAKLPKEIRFAIEFR
                     NREWLVDWTFEELEKTEVALCLVEGGWIPREMMFEAIGKLKTDFAYIRFMGERDLTTF
                     DRIVRPQDAALQIWREEIEKIKAKDIFIYFSNFYEGFAPESVNKLREMFGQTTVDAEE
                     LENQGSLF"
     gene            700..2496
                     /gene="sppA"
                     /locus_tag="VGB68_11610"
     CDS             700..2496
                     /gene="sppA"
                     /locus_tag="VGB68_11610"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEY0049928.1"
                     /translation="MSKTGKILLITGSVLVVFFLIAIIGIALLAESLGKPDVPNNSVL
                     VLNVSGELPDYSAEDPTAKLFGMESPQSFSSLMTQMRKAKVDSRIGAVLLNVNFPGIG
                     WGKADELRAAITDLRASGKPVYAYMEIGMNKEYYIASAAEKVFLPPAGDIYINGFAAE
                     VTFYRGSLDKLGIEPEVIAIGKYKNAPDQFTRKEMSEAHSEVMNAIIDEYYNRFINGI
                     AESRKKSPEDVKAMIDNAPYHAPEAKQLGLIDEAAYRDQVYDELKKRLGYKEGDKIRT
                     VSGSSYKEISSDSLGLNNGERVAVVYASGAINVGRSSRSTFGGESLGSDTLVKAINDA
                     AEDSSIKAIVLRVDSPGGSALASDLMWHAIENAKAKKPVVVSMADVAASGGYYIACNA
                     NKIVAEPSTLTGSIGVFMGKPSLKGFNDWLGISTQYVQRGKNSGIFRTGDKWTEEEKK
                     KMEEQTNKIYWDDFVPKVAKGRGKTNEDVNTLAQGRVWTGAQAKERGLVDEIGGLEKS
                     IEIAKQLANLPADKDVRRVVFPEPRTFFEEYFGGSGEDSTFAQSKDRQAQEALVKSLP
                     EDMRRALRYAELFDNMRNGEAMLMLPYEFEIK"
     gene            2808..3719
                     /locus_tag="VGB68_11615"
     CDS             2808..3719
                     /locus_tag="VGB68_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049929.1"
                     /translation="MLVSASLTGFAIAQDTTTRTTTTTTTEKKVVQNADGSYSVIEYP
                     VDKEVTVELTPYGVQGAKGWARVMRKADGTTVALDLSGLPADSKNYYVYAVDPAGKVT
                     LLGPAGVNNGMSKTTFTTPMNQFMLVLSPNEGLSAFNTDTPVVFRSSVPAGYAVVPAA
                     VTDSMENKQVATSTNINSTYQVPLLDVPKFSKTAEIRINFSGDLQGLKGKAYVDPTKG
                     GVTKVKMRFDDMKMAPKNKRYVLWASSPDGKYTKLGQVINTGERQESEIRSETALNNF
                     GLFVTVEDTDVTQPTSTVYAPFIIGTP"
     gene            3890..4819
                     /locus_tag="VGB68_11620"
     CDS             3890..4819
                     /locus_tag="VGB68_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049930.1"
                     /translation="MAEQQNSGTGNTGNFGNQSTGSGGSGVSTQAAKDTTGGASGSEG
                     SGTLGNVTTKVKGLYQTAADSQVGQVAKDALGQVKDNATTAIDDKKANLAQGLTSVAD
                     TLRQVGDTLKPSEGEPPANKVAELTTQYTESLSQQVEKISGYLERQDLRGLMTDVEKF
                     ARRNPAVFLGGAFALGILAARFLKTSNPNQALIRTSDVSDFDSGFDTTPNSLGSASTG
                     SSFDTSSTGSLGQTGSSIGDKGDSFGETSGAFDSEADNIRQFNTGSGAGSSTTSGSGT
                     GSTATGSTDTKDSSSTSPKPSNKTGGDFGSSKF"
     gene            4844..5245
                     /locus_tag="VGB68_11625"
     CDS             4844..5245
                     /locus_tag="VGB68_11625"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEY0049931.1"
                     /translation="MAQPVVTKEERSLGELFSELASETSTLVRQEVALAQTELTQKAT
                     KVGKNVGFLVVGGAVGYAALLAIIAAVIIGLSHFIPAWVSALIVGVVVGIVAYLLISS
                     ALAALKKTDLTPRETVETLKEDAQWLKNQVS"
     gene            5221..6363
                     /locus_tag="VGB68_11630"
     CDS             5221..6363
                     /locus_tag="VGB68_11630"
                     /inference="COORDINATES: protein motif:HMM:NF023697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HEY0049932.1"
                     /translation="MAEKSSELTKDATGGEGILTDSDKEVISTVAGSSDRDAFIEKYS
                     DDYAAGDSEIDDITTNTSEDAPEETEHLKAQIEETRSQMGETIDAIQERLSFQNISDQ
                     VKEQVSEQISSAFETAKDTVYDATIRKAGDFMQSMNKSVNKSLDGISKSEAMHVMRAN
                     PLALSLIGLGVGLLLMNGYKKKSSYRYADGNRGRQGKGAYASGGEAYSNQPSMLKSAQ
                     DKITGTASTAYESVGNAASGAYDSVSNVAGSTYESVSGAATNAYSSVTGAASTAVSGV
                     TDYAGRAYDKVGDLRTQATEQYDYYIEENPLAVGAVALALGAAVGMALPSTRVESHLM
                     GEAKQNLLQKAQDTAGDLVDRVKQVANEAQKTITDEVKTQAQGLKQ"
BASE COUNT         1812 a         1530 c         1748 g         1413 t
ORIGIN      
        1 ctcaaggaaa agctcgccgc cgttttgatt caaatgccgc cgcagtttga aggctcgaag
       61 gaaaacgcgc ggaatttgcg aaattttctg gcgaaattgc cgaaagagat tcggtttgcg
      121 attgaattca gaaaccgcga atggctggtc gattggacgt ttgaagaact ggaaaaaacg
      181 gaggtcgcgc tgtgtctggt cgaaggcggc tggattccgc gcgaaatgat gtttgaagca
      241 atcggcaaat tgaagaccga ttttgcatac atccgcttta tgggcgagcg cgatttgacg
      301 acgttcgata gaatcgtgcg cccgcaggac gccgccctgc agatttggcg cgaagaaatc
      361 gagaaaataa aagcgaaaga catttttatt tatttcagca atttttacga aggcttcgcg
      421 ccggaaagcg tcaataaact gcgggaaatg ttcggtcaaa caacggttga cgccgaagaa
      481 ctggaaaatc aaggctcgct gttttaattg aaaaatgtag caataattcg aaaatgcggc
      541 aataatcgta gagacgcgtt gcggcgcgcc tcaacaaaca tattaacgat tactcgttcg
      601 gcaaacatct tgacgattat tcggtaaagc aaactttttc gttttttaaa cgtaaaataa
      661 agagaaattt agtttcggag tatcttttac aaaatggcaa tgtctaaaac aggaaaaatt
      721 cttcttatca ccggcagcgt tctggtcgtt ttctttctga tagcgattat cggcatcgcg
      781 ctgctggcgg aatccctcgg caaacctgac gtacctaaca acagcgtcct ggtgctcaac
      841 gtatcgggcg aactgcccga ttactcggcg gaagacccga cggcgaagct tttcgggatg
      901 gaatcgccgc aatccttttc gagcctgatg acgcagatgc ggaaggcgaa agtcgattcg
      961 cgcatcggcg cggttttgct caacgtcaat tttcccggca tcggctgggg caaagcggac
     1021 gagctgcgcg cggcgattac cgatttgcgc gcgtccggta aaccggttta tgcctatatg
     1081 gaaatcggta tgaacaagga gtattacatt gcttcggcgg cggagaaagt ttttctgcct
     1141 ccggcgggcg atatttacat taacggtttt gcggcggaag tgacgtttta tcgcggctcg
     1201 ctcgacaagc tcggcatcga gccggaagtc atcgccatcg gcaaatataa aaacgcgccc
     1261 gaccagttta cgcgcaagga aatgagcgaa gcgcattcgg aagtgatgaa cgcgattatc
     1321 gacgaatact acaatcgttt tatcaacggc attgccgaat cgcgcaaaaa atcgcccgaa
     1381 gacgtgaaag cgatgattga caatgcgccg tatcacgcgc cggaagccaa acagctcggc
     1441 ttgattgacg aagccgctta ccgcgaccag gtttacgacg aattgaaaaa acgtctgggc
     1501 tataaagaag gcgataaaat ccgcaccgtc agcggaagca gctacaagga aatttcttcg
     1561 gattcgctcg gtttgaacaa cggcgagcgc gtggcggtcg tttacgcttc gggcgcaatc
     1621 aacgtcgggc gctccagccg cagcaccttc ggcggcgaat cgctcggttc ggatacttta
     1681 gtcaaagcta tcaacgacgc ggcggaagac agttccatca aagcgattgt gctgcgcgtc
     1741 gacagcccgg gcggctcggc gctcgcgtcg gatttgatgt ggcacgcgat tgagaacgcg
     1801 aaagcgaaaa agccggttgt cgtttcgatg gctgacgtcg ccgcatcggg cggttattac
     1861 atcgcctgca acgccaataa aatcgtcgcc gagccttcga ctttgaccgg ctcaatcggc
     1921 gtgtttatgg gcaagccgtc gctgaaaggc tttaacgatt ggctcggcat cagtactcaa
     1981 tacgtacagc gcggcaaaaa ttccggaatc tttcgcaccg gcgacaagtg gacggaagaa
     2041 gaaaagaaaa aaatggaaga gcagaccaac aaaatctact gggacgattt tgtgccgaag
     2101 gtcgccaagg gtcgcggaaa aaccaacgag gacgtcaaca cgctcgcgca ggggcgcgtc
     2161 tggacgggcg cgcaagcgaa agagcgagga ttggttgacg aaatcggcgg gctggaaaaa
     2221 tcaatcgaaa tcgctaaaca attagccaat ctgcccgccg acaaagacgt gcgccgcgtc
     2281 gtttttcccg aaccgcgcac gtttttcgaa gagtatttcg gcggcagcgg cgaagacagc
     2341 actttcgcgc aatcgaaaga ccgccaggcg caggaagctc tggtcaaatc gcttcccgaa
     2401 gatatgcgcc gcgctttgcg ctacgccgag cttttcgaca atatgcgaaa cggcgaagcg
     2461 atgctgatgc tgccttacga atttgaaata aaataacagc tgaagagtga aaagccgaag
     2521 gcgatgggaa agaaacgctc tttcttgtcg ccttcggctt tttatctatt actttgaaca
     2581 atctcaatac attttctgtc aagaatcaat acacttttcg gctccgccag cttcggaaaa
     2641 ttgcttcaaa atccgcttaa acttccaaaa caaagagatt gcacagtttt ggcacggtca
     2701 ttgcactcag accagccaaa cggacggatt cgtgtgaaat gtttgcacga ttagatttca
     2761 aggaggtttt tatgatcaaa aacaagttat ttggactcgg agcagcgatg ttagtttctg
     2821 cttcactaac cggttttgca atcgcgcaag acaccaccac aagaaccacg accacgacca
     2881 caaccgagaa aaaagtagta caaaatgctg acggaagcta ttcggttatc gaatatcccg
     2941 tcgataaaga agtgacagtc gaattgactc cgtacggcgt tcagggcgct aaaggctggg
     3001 cgcgcgtgat gcgtaaagcc gacggcacga ccgtcgccct cgatttgtca ggacttccgg
     3061 cggattcgaa aaattattat gtttatgccg ttgacccggc aggaaaagtg actttactcg
     3121 gaccggcagg agttaacaac ggcatgagta aaactacttt tacgacgccg atgaatcaat
     3181 ttatgctggt gctttcgccg aacgaaggct tgtcggcatt taataccgat acgccggttg
     3241 tcttccgcag ttccgttccg gctggttacg cagtcgttcc ggcggcagtc accgattcga
     3301 tggaaaacaa acaggtagcg acttcgacca acatcaattc gacgtatcaa gttccgctgc
     3361 tcgacgttcc gaagttttca aaaacggcgg aaatcagaat caattttagc ggtgatttgc
     3421 agggcttaaa aggtaaagcc tatgttgacc cgaccaaggg cggcgtcacc aaagtcaaaa
     3481 tgcgttttga cgatatgaag atggctccga aaaataaacg atatgttttg tgggcttctt
     3541 cgccggacgg aaaatatacc aagctcggtc aggttatcaa caccggcgag cgtcaggaat
     3601 ccgaaatcag aagcgaaacc gctttaaata atttcggtct attcgtaacg gttgaagaca
     3661 cggacgtaac acagccgacg agcacggttt atgcaccgtt tattatcggc actccgtaat
     3721 tcggtcgaat tagtttgata attcgattta agcggcaggt tcgccctaag aacttgtcgc
     3781 ttttattgac gataaaacag cgtggtcgtt tttgacggtt aatagattgt ctttagtaaa
     3841 acgactaaat attaattaat aaaactaacg ggaaaggggt taatttatta tggcagaaca
     3901 acaaaattca ggaacgggaa atacaggtaa tttcggaaat caatcaacag gcagcggcgg
     3961 gagcggagtt tccacgcagg cggcaaaaga tacaaccggc ggcgcgtcag gttcggaagg
     4021 ctcgggtacc ttgggcaatg taacaaccaa ggtcaaaggt ctttaccaga cggcggcgga
     4081 ttctcaggtc ggacaagtgg cgaaagacgc gctcggacag gtcaaagata acgcgacgac
     4141 ggcaatcgac gataaaaaag cgaatttagc tcagggctta accagcgttg ccgacacact
     4201 tcgacaggtc ggtgacacat tgaaaccttc ggaaggcgaa ccgccggcaa acaaagtggc
     4261 tgaactgacc acacaataca ccgaatcatt gtcacagcag gtcgaaaaaa tttcgggtta
     4321 tctcgaacgc caggatttac gcggtttgat gaccgatgtc gaaaaatttg cgcggcgcaa
     4381 tccggcagtc tttctcggcg gcgcctttgc gctcggaatt ctggcggcgc gttttctgaa
     4441 aacttcaaat ccgaatcagg ctttgattcg aacttcggat gtgagcgatt tcgatagcgg
     4501 ttttgataca acgccgaaca gtctcggcag cgcatcgacc ggaagctcct tcgatacgtc
     4561 atctaccggc tcgctgggtc aaaccggaag ctcgataggc gacaagggag attcattcgg
     4621 cgaaaccagc ggcgctttcg acagcgaagc agacaacatc agacagttta ataccggttc
     4681 aggcgcgggt tcgagcacga catcaggctc cggcacaggt tcaaccgcta caggttcgac
     4741 cgacacgaaa gactcgtcct ccacttcgcc gaaaccgtca aataaaaccg gcggggattt
     4801 cggctcgtcg aaattttaag agggaaaaca ggaggataat tttatggcac aaccagtcgt
     4861 caccaaagaa gagcgcagtt taggcgaact gttttccgag cttgccagcg aaaccagcac
     4921 actagtccgg caggaagtcg cgctggcgca gacggaactg acgcagaaag caaccaaagt
     4981 cggtaaaaat gtcggctttc tggttgtcgg cggcgcggtc gggtatgcgg cgctgctcgc
     5041 cattatcgcg gcggtaatca tcggtctgtc gcattttatt ccggcttggg tatcggcgtt
     5101 aatcgtcggc gtagtggtcg gtatcgttgc ttacctgctg atttcatcgg cgttagccgc
     5161 cttgaaaaag acggatttga cgccgcgtga aactgtcgaa acattgaagg aggatgcaca
     5221 atggctgaaa aatcaagtga gttaacgaaa gacgcgaccg gcggcgaagg aatattgacc
     5281 gactcggaca aggaagtaat ttcgacggtt gccggttcgt ctgaccgcga tgcttttatc
     5341 gaaaaatatt cggacgatta cgcggcgggc gacagtgaaa tcgatgatat tacgaccaac
     5401 accagcgagg acgcgcccga agaaaccgaa catttaaaag cgcaaatcga ggaaacgcgc
     5461 agtcagatgg gcgagaccat cgacgcgatt caggaaagac tgagctttca gaatatttcc
     5521 gaccaggtca aggaacaggt ttccgaacaa atcagcagcg cgtttgaaac ggcgaaggac
     5581 accgtttatg acgcgactat cagaaaggcg ggagatttta tgcaaagtat gaacaaaagt
     5641 gtaaataaaa gtttagacgg aatttcaaaa tcagaagcga tgcatgtgat gcgtgccaat
     5701 cctttggcat tatcgcttat cggattgggc gtcgggctgc ttttaatgaa cggctataag
     5761 aaaaaatctt cgtatcgcta tgccgacggc aaccgcggtc gtcagggcaa aggcgcatat
     5821 gcgtcgggcg gcgaagctta cagcaatcag ccttcgatgc tgaaatcggc gcaggataaa
     5881 atcaccggaa cggcgagcac cgcttatgaa agcgtcggta atgcggcaag cggcgcttat
     5941 gacagcgtct cgaacgttgc cggaagcact tacgaaagcg tttcaggcgc ggcgactaat
     6001 gcttacagca gcgtcaccgg cgcggcaagc actgccgttt cgggcgttac ggattatgcc
     6061 ggacgcgctt atgataaagt cggtgattta cgcacgcagg cgaccgagca atacgattat
     6121 tacatcgaag aaaatccgct cgcggtcggc gcggtagctt tggctttggg cgcggcggtc
     6181 ggtatggcgc ttccttcgac gcgtgtcgaa agtcatttaa tgggtgaagc caaacaaaat
     6241 cttctgcaaa aagcgcagga tacggcgggc gacctcgttg accgcgtgaa acaggttgct
     6301 aatgaagcgc agaaaacaat taccgatgaa gtcaaaacac aagcgcaggg tttaaagcag
     6361 taaaaattta cggcgagagc ttacatcaat caaagcggaa ggcgaaagaa accataaaaa
     6421 atttctttcg ccttccgctg ttttattctt aagaaatcac aaaagaggca aagaaatttt
     6481 gccgcgaaag gacgcaaaaa aga
//
LOCUS       DASWNE010000592         5524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_512253, whole genome shotgun sequence.
ACCESSION   DASWNE010000592 DASWNE010000000
VERSION     DASWNE010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5524
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512253"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..953)
                     /locus_tag="VGB68_11635"
     CDS             complement(87..953)
                     /locus_tag="VGB68_11635"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HEY0049933.1"
                     /translation="MPIHNTQAPEIHESIAPEIRKAFDYPFCVRDVPAEEIHEGRYIT
                     RFARTPEEIDAALRLRFTIFNLELNEGLESSFLTERDQDEFDQTCHHLIVIEKATNAL
                     VGTYRLRTIEQAETARGFYSASEFKIEDLPPEVLNQSLEIGRACIAREHRNSRVLFLL
                     WKGLALYSILKNKRYLFGCCSLFTLDCTEGKKALRQLVRDGFFHEVFRVAPREDCACR
                     AEDFLTADSIEEIELPKLFFTYLRIGAKICGEPVIDRHFKTIDFFVIFDATTIEPRYY
                     QMFFSQFEHLKK"
     gene            complement(1161..2468)
                     /locus_tag="VGB68_11640"
     CDS             complement(1161..2468)
                     /locus_tag="VGB68_11640"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY0049934.1"
                     /translation="MEKIYRDSVHNIISLKTDTDEGRLMIRLVDTAEFQRLRRIKQLG
                     LALFTYQGAEHSRFTHSLGVLHLATRILEKLGTKYKISDEARIAVRCAALLHDIGHGA
                     FSHVIEGILNFHHEDFTIQAVLSGETEVGQALRGFSSELPAAVADIICGKYRPMALAQ
                     LVSSQLDVDRMDYLLRDSLMTGAKYGIYDLEWIIKSLEIDEENDRLYISAEGIYAVED
                     YLQARYYMFRQVYFHRSLRSAEVVLRSLLRRALEIFQSGRAVWFAHGTPFEKILRGEK
                     LSLAEHLEMDDSDVMFHIKQWQRSADAILSDLSKRFIHRRLFKAFDIDMPNEERRDFL
                     EKARRAVEKAGFDASYYFVEDKTGDVPYYFYTKDADKPKNLIYVQEGYARSTIREISE
                     ISAAVRGLQKGYVIHRVCFPHELNVEISRLYHGYGDAAADAHG"
     gene            2719..>5524
                     /locus_tag="VGB68_11645"
     CDS             2719..>5524
                     /locus_tag="VGB68_11645"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0049935.1"
                     /translation="MKTILQILTVFVFALGLAIIPVTAQTSTTGNIEGTVTDPSGAVV
                     PGASVTVSGGNLVRAITVTANDEGFFRVGNVPPGRYSVKVEAAGKGFATYENQNIEVN
                     LSRTTTVDTQLTPASTGATVDVVAGSEIDTTQNTSGTNVSTEQFSNFPTQRTVQSLYT
                     IAPTVTRSGLRDASGRDRDPSVAGSSGPENNYILDGVNTTDPAFGGSGANLPFEFVQE
                     VEVKTGSYGAEYGLSTGGIFNVITKSGTNEFRGEVYGYFTGKSMVRDARNFSFTGLSP
                     SGFSEIDAGFGIGGPIIKDKLTFYGSFNPQRRNNSYLTQTFRTEVENKINTPFYAGKI
                     TWSINDRNTFNFSTFGDFTKEEGHLFAGSGFGANLNSFEGVRETGGSNYAFRLNSTFN
                     QNFIGEFSFGMHFQVNNVIPGSDVLDTPLVTDNFAVLRANGTVAPVVQTGVLAGGTVA
                     TPGTNTGFVDFVYSPGGSLQRNFVRQGFGLYQDQTRNRYEAAARLQSIFGRHTLKYGF
                     EYYRNIYDINQTSTGPSNVFANPLNVPPLNQTDAANRNVSGFRVTNNFSVCTVRGTQV
                     VCPSGTATNILTPAIAANQVPGITSVITGVITPQEALNNPFLVRTTTRVRDFKLIADT
                     KTNVESFYVQDDIKVSNTIQLNIGARWDFQQAYGNGGVEYLKLNNFWHNLQPRIGFIW
                     DFTGKGKGKVFANYARYLETPIPLDLNVRAGSGDAQTDKNFNVNTLNAPLNATVATGF
                     GTRNLGHDATPIDADLKPQTVNEWNVGIEYEAMKDLVLGVRGIYRPQGTVIEDGSFDD
                     GTTYFIFNPGESATEQAACDFGQCFGRARRFYRALEFTATRRFNNNFSFIASYVFSSL
                     TGNYEGLFRNDNGQSDPNITSLFDLTSLLNNTYGRLPNDRPHQFKFNGTYVTPWRVVL
                     SGNFYLQSGAPFNALIPHPVY"
BASE COUNT         1342 a         1487 c         1375 g         1320 t
ORIGIN      
        1 cgtcagccgt cagcttacgg ttgaatataa attacgttcc ggattttcac tcgaccataa
       61 gctgaccgcc gacggctgac ggctgattac ttttttaaat gctcgaactg gctgaaaaac
      121 atttgataat agcgcggctc gatggtcgtc gcgtcgaaaa tcacgaaaaa gtcgatcgtt
      181 ttaaaatgac ggtcgatgac cggctcgccg cagattttcg cgccgatgcg caggtatgta
      241 aaaaaaagct tcggcaattc gatttcttcg attgaatcgg cggtcagaaa atcttcggcg
      301 cgacaggcgc aatcctcgcg cggcgcgacg cgaaagactt cgtgaaaaaa accgtcgcgg
      361 acgagctgcc tgagagcttt tttgccttcc gtgcagtcga gcgtaaacag cgagcagcag
      421 ccgaaaagat aacgcttgtt tttcaaaatc gagtataaag ccaagccttt ccagagcaga
      481 aaaagcacgc gcgagttgcg atgctcgcgg gcgatacagg cgcgcccgat ttccagcgat
      541 tgattcaaaa cttccggcgg caaatcttcg attttaaatt cgctggctga ataaaagccg
      601 cgcgccgttt cagcctgctc gatagtgcgc agccggtatg tgccgaccag cgcgttcgtc
      661 gctttttcaa ttacaatcag gtgatggcag gtctggtcaa attcgtcctg gtcgcgttcg
      721 gtcagaaacg acgattccag accttcgttt aattccagat taaaaatcgt gaagcgcaga
      781 cgcagagccg cgtcgatttc ttccggcgtg cgggcaaagc gcgtgatgta tcgaccttcg
      841 tggatttctt cggcgggaac gtcgcggacg caaaacgggt aatcgaacgc cttccggatt
      901 tcaggcgcga ttgattcgtg aatttcaggt gcttgcgtgt tatgaattgg cattgataaa
      961 agtttatggt ttcggacaat ttcgggcggt tacggaacta ttaaaatttt cctgaatttt
     1021 ccgcgacttc ccgccgccgt ccgaaacttt caacttttct ggaattgtag cgcactaata
     1081 tataaatcgc cagagatttt ccgtgcctgc cgagccgcaa tataaaagat gtaagcaaaa
     1141 gttaagattt caagcgcgtt tcacccgtgc gcgtcagccg ccgcgtcgcc gtagccgtga
     1201 taaagtctgg aaatttctac atttaattcg tgcggaaaac agacgcggtg gatgacgtaa
     1261 cctttctgaa gcccgcgcac cgccgcgctg atttccgaaa tctcgcgaat ggtcgagcgc
     1321 gcgtagcctt cctgcacgta gattagattt ttcggtttgt ctgcatcttt cgtgtagaaa
     1381 taatacggga cgtcgccggt tttgtcctcg acaaaataat agctggcgtc aaagcccgct
     1441 ttttcgaccg ctcgccgggc tttttccaga aaatcccggc gttcttcgtt cggcatatcg
     1501 atgtcgaagg ctttaaaaag gcgtcgatgg ataaagcgtt tcgacaaatc cgataaaatc
     1561 gcgtccgcgc tgcgctgcca ttgtttgatg tgaaacatca cgtcggaatc gtccatttcc
     1621 agatgttcgg cgagcgacag tttttcgccg cgcaggattt tttcgaacgg cgttccgtga
     1681 gcgaaccaga cggctctgcc ggattgaaaa atctccagcg cgcgccgcag aagcgagcgc
     1741 agaacgactt ccgccgagcg caggctgcga tgaaaataaa cctgccggaa catataataa
     1801 cgcgcctgca aataatcttc gacggcgtaa attccttcag ccgagatata caggcggtcg
     1861 ttttcctcgt cgatttccag cgatttgata atccattcga ggtcgtagat tccgtatttc
     1921 gcgcccgtca ttaaagaatc gcgcagaaga taatccatcc ggtcgacgtc gagctgcgac
     1981 gagacgagct gcgccagcgc catcgggcga tattttccgc agatgatgtc ggcgacggcg
     2041 gcgggcagct ccgaggaaaa gccgcgcaaa gcctgcccga cttccgtctc gccgcttaaa
     2101 accgcctgaa tcgtaaaatc ttcgtgatga aaatttaaaa ttccctcaat cacgtgcgaa
     2161 aaagctccgt gcccgatgtc gtgcagcagc gcggcgcagc gcaccgcaat gcgcgcttca
     2221 tccgaaattt tgtattttgt gcctaatttt tccaaaatcc gcgtcgccag atgcagcacg
     2281 ccgaggctgt gcgtaaagcg cgaatgctcc gcgccctgat aggtgaaaag cgccagcccc
     2341 agctgtttga tgcgccgcag acgctgaaat tccgccgtat cgaccagccg aatcatcaat
     2401 ctgccttcgt ccgtgtcggt tttcagacta ataatattgt gaaccgaatc gcggtaaatt
     2461 ttttccattg ttctcgataa aattctagca ggaattcaaa cttatggata taaatcaatt
     2521 tttttagttc tgcggcgcgc gtcagatttt tattttacac cgtaatttat taataattac
     2581 aaggttttac gaggtttttg ccgaaaggca taatagttgc tagataaacg ccagaaacat
     2641 agagtttctc cccaattatt cgcaaattac ctaattgttt gtaaattttt aggctacatt
     2701 aaagaaagga aagaaaatat gaaaacaatc ctgcaaattt taactgtttt cgtttttgcg
     2761 ttgggattag cgataatccc ggttacggcg cagacttcga cgacaggaaa cattgaaggt
     2821 actgttaccg acccgtcggg tgcggtcgtt cccggcgcat ctgtgacggt ttcgggcggc
     2881 aacctggttc gcgcgataac ggtaacagcc aatgacgaag gatttttccg cgtaggcaac
     2941 gtgccgccgg gcagatattc cgtgaaagtg gaagctgccg gaaaaggctt cgctacatac
     3001 gaaaaccaaa acatcgaagt caatctgtcc agaacgacta cggtcgatac gcaactgacg
     3061 cccgcctcga caggcgcgac cgttgacgtt gtcgccggtt ctgagattga caccacccaa
     3121 aacacttcag gaacgaacgt ctcgaccgaa cagttcagca acttcccgac gcagcgcacc
     3181 gtgcagtcgc tttatacgat tgcgccgacc gtgacgcgct cgggcttgcg cgacgcatcg
     3241 ggacgtgacc gcgacccgtc ggttgccggt tcgtcaggtc ctgaaaacaa ctacatcctc
     3301 gacggcgtga acacgaccga ccccgctttc ggcggttcgg gcgcgaacct tccgttcgag
     3361 ttcgtccagg aagttgaagt taaaaccggt tcatacggcg ccgaatacgg gctgtcgacc
     3421 ggcggtatct tcaacgttat caccaagagc ggaaccaacg aattccgcgg cgaagtttac
     3481 ggttatttca cgggcaaaag catggttcgt gacgcgagaa acttctcgtt caccggtctt
     3541 tcgccgagcg gcttttcgga aatcgacgcc ggtttcggta tcggcggtcc gattatcaag
     3601 gataaactga ccttctacgg ttcgttcaat ccgcagcgtc gtaacaacag ctatctgacg
     3661 cagaccttcc gcaccgaagt cgaaaacaaa attaacactc cgttttacgc gggcaaaata
     3721 acctggagca tcaacgacag aaacacgttc aacttctcga cctttggcga ctttaccaaa
     3781 gaagagggtc acttgtttgc cggttcgggc ttcggcgcga atctgaacag cttcgaaggc
     3841 gtccgggaaa ccggcggctc gaactatgct ttccgtttga attcaacctt taatcaaaac
     3901 tttatcggtg aattttcatt cggtatgcac ttccaggtca acaacgttat tccgggcagc
     3961 gatgttctgg acactccttt agtgacggac aactttgcgg ttctccgcgc caacggaaca
     4021 gttgctccgg ttgtccagac cggagtttta gccggcggca cggtcgccac tccgggcacc
     4081 aacaccggct tcgtagattt cgtctattcg ccgggcggct cgctccagcg caacttcgtc
     4141 cgacagggct tcggtcttta tcaagaccag acccgcaatc gttacgaagc ggcggctaga
     4201 ctgcaaagca tttttggacg tcatacttta aaatacggtt ttgaatacta ccgtaatatc
     4261 tatgacatca accagacttc gaccggtccg agcaacgtgt ttgccaatcc gctcaacgtt
     4321 ccgccgctta atcagactga tgctgcgaac agaaacgtgt ccggcttccg cgtgacgaac
     4381 aacttcagcg tttgtaccgt tcgcggtacg caggtcgttt gtccgagcgg aaccgcgaca
     4441 aatatcttaa ctccggcgat cgcggctaat caggttccgg gaatcaccag cgttatcacc
     4501 ggagtgatta ctccgcagga agcgcttaac aatccgttcc tggttcgcac caccactcgc
     4561 gtccgcgact ttaaattgat tgcggatacg aaaaccaacg ttgaaagctt ctacgttcag
     4621 gacgacatca aagtcagcaa caccatccaa cttaacatcg gcgcacgctg ggatttccag
     4681 caagcttatg gtaacggcgg agtggaatac ctgaaattga acaacttctg gcacaacctg
     4741 cagccccgca tcggtttcat ctgggatttc acgggtaaag gcaaaggtaa agtctttgct
     4801 aactacgccc gttatctgga aacgccgatt ccgctcgacc tgaacgttcg cgcgggcagc
     4861 ggcgatgctc aaaccgataa aaactttaac gttaacacct tgaacgcgcc tttgaacgcg
     4921 acagtcgcga ccggcttcgg tacccgtaac ctgggtcacg atgctactcc gattgacgcg
     4981 gatctgaagc cgcagacggt taacgaatgg aacgtcggta tcgaatatga agcgatgaaa
     5041 gacctcgttc tcggagtccg cggcatttac cgcccgcaag gcacggtaat tgaagacggt
     5101 tcgttcgacg acggtaccac ttacttcatc ttcaatccgg gcgaaagcgc gactgaacaa
     5161 gctgcttgcg atttcggaca atgcttcgga cgcgcccgcc gcttctatcg cgctctggaa
     5221 ttcacggcta cccgccgctt caacaacaac ttctcgttca tcgcttcgta tgtgttctcg
     5281 agcttgaccg gcaattacga aggcttgttc cgtaacgaca acggacagtc cgacccgaac
     5341 atcacttcgt tgttcgattt gaccagcctg ctcaacaaca cctacggacg tctgccgaac
     5401 gaccgtccgc atcaattcaa gtttaacggt acatacgtga caccgtggag agttgtcctt
     5461 tccggcaact tctacctcca gtccggcgcg ccgttcaacg ctttgattcc gcacccggtt
     5521 tacg
//
LOCUS       DASWNE010000593         1595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_512261, whole genome shotgun sequence.
ACCESSION   DASWNE010000593 DASWNE010000000
VERSION     DASWNE010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512261"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VGB68_11650"
     CDS             <1..408
                     /locus_tag="VGB68_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049936.1"
                     /translation="CYVENPGTDRTPSANHIVSGTINFCNRFFDGTSEASDDNRVRIV
                     LHEMFHWLAPRGLAILDTQTHSDMKKFGCGTDTDKMYGEDDALHLATSTGCWGDPKYH
                     RGMAARNNDNYAYFILQFGNAVYDKRLTRFPQK"
     gene            complement(445..981)
                     /locus_tag="VGB68_11655"
     CDS             complement(445..981)
                     /locus_tag="VGB68_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049937.1"
                     /translation="MRNKLASALLIASVFVWGIWCGGQVFNELMSVPKWSASPPESLK
                     AYDALPSRGGLPFFPLFNPLFVVLAIGAAIAAWKSARRARIWLALSAATSLVLFIALV
                     FYLAPLIGSMFQHSIAGDLAAADIVAGVERWKLGNRIRLIAELSGFVFSIIALRVWSA
                     ENAFSGERLAIQTNKADG"
     gene            complement(1082..1204)
                     /locus_tag="VGB68_11660"
     CDS             complement(1082..1204)
                     /locus_tag="VGB68_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049938.1"
                     /translation="MLIVRPSAEAEWYWINSANNQSSGLQFGGGADKPARAQQA"
     gene            complement(1355..>1595)
                     /locus_tag="VGB68_11665"
     CDS             complement(1355..>1595)
                     /locus_tag="VGB68_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049939.1"
                     /translation="ALSALSALMDATTKSTFVEALQKFAGTDSTALYQAMIGLDNIGE
                     DVFAGRQSKSFTMVEENTRLAKEYLKRQDKNENPS"
BASE COUNT          403 a          409 c          378 g          405 t
ORIGIN      
        1 tgttatgtgg aaaatccggg aaccgaccga acgccttcag ccaatcacat cgtttcgggc
       61 acgataaatt tctgcaatcg tttttttgac gggacgtcgg aagcgagcga tgacaaccgc
      121 gtcagaatcg tcctgcacga aatgtttcac tggctggctc cgcgcggctt ggcgattctg
      181 gatacgcaga cgcattcgga tatgaaaaaa ttcggctgcg gaacggatac cgacaaaatg
      241 tacggcgagg acgacgcgct tcatttagcg acttcgaccg gttgttgggg cgatccgaaa
      301 tatcaccgcg ggatggcggc gcgtaataac gataattacg cttattttat attgcagttc
      361 ggcaacgccg tttatgacaa acggctgacg cgcttcccgc aaaaatagcg gcaaaaatct
      421 ttccgctatc tgagccgacg gttatcagcc gtcggcttta tttgtttgaa tagcgagccg
      481 ttcgccggag aaagcatttt cggctgacca aactcgcagc gcgatgattg aaaagacaaa
      541 tcctgatagt tcggctatta agcggattcg gttgcctaat ttccaacgct caactccggc
      601 aacgatgtcg gcggctgcta aatcgcccgc aatgctgtgc tgaaacatac ttccgatgag
      661 cggcgcaaga taaaaaacta atgcgataaa caaaaccagc gaagttgccg ccgataatgc
      721 cagccagatt cttgcccggc gcgccgattt ccaagccgcg attgccgcgc cgattgccag
      781 aacgacaaac aatgggttga aaagcggaaa aaacggcagc ccgccgcggc tcggcagcgc
      841 gtcgtaagcc tttagcgatt cgggcggcga tgcgctccat ttcggcacgc tcatcaattc
      901 gttgaaaact tgtccgccgc accaaattcc ccaaacgaaa acggatgcga ttaagagcgc
      961 agatgctaat ttatttctca taaattccga ctcgacctgg tttcacatta agcgggctaa
     1021 ctttgaatta gcgcaaattg cattccgggt gataaattac cgcgaatcag ccgtccgatt
     1081 ttcaggcttg ctgagcgcgt gcgggtttat cagcgccgcc gccgaattgc agaccgctgc
     1141 tttgattatt cgcgctatta atccaatacc attcagcctc cgcgctcggg cgaactatca
     1201 acatttaacc aaaattaata aggtgactga tatatttcaa aaggcaatgt ttgcctatac
     1261 atttcatttg tccccggttt tcttttaccg gctcaaaaag ggcgtttttg atactgatag
     1321 agagaggctg tccgccatta cgtttatgga attgtcagct tggattttca tttttatcct
     1381 gcctctttaa atactccttc gctaaccgtg tattttcctc taccattgta aaggatttac
     1441 tttgcctgcc ggcgaaaacg tcttctccga tgttgtccaa gccaatcatc gcttgatata
     1501 aggcggtgct gtcagtaccc gcaaactttt gcagcgcctc cacaaaagtc gattttgttg
     1561 tcgcatccat taaagcactt aacgccgaaa gtgcg
//
LOCUS       DASWNE010000594         3982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_656097, whole genome shotgun sequence.
ACCESSION   DASWNE010000594 DASWNE010000000
VERSION     DASWNE010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656097"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1672
                     /locus_tag="VGB68_11670"
     CDS             <1..1672
                     /locus_tag="VGB68_11670"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY0049940.1"
                     /translation="TDTDARTLEPVPAVAARWTASEDFKTWTFYLRKDAKWSNGETVT
                     AKDFVRSWQRLTSLGEKVQQRKLLKNIIGMDTENVLPVFAGEEIDFSATSPNPASRLP
                     AQSLQSNSNSNAAAPAPPQKVKPDEKKTEIPKTENKTESKTAKEEEVKFGVEAVDNHT
                     LKVSLVQPDKDFPALVAHPIFRPIYGDGKEFETAELSANIITNGAFRIFSIGRDGVTL
                     DRAEYYWNKAEINLERVRFVPTESAEIALEGYRAGEIDAITNANFEPLALKLLTPYDD
                     FRRTKHSALNFYEFNSKNHPFGDRRIREALAIAIERERLTEDEMDGATEPAFNYLPFD
                     EPRLMQNVEKAKTLLAEAGFAESEKFPVVRLLINRNDLQRRIARSVAKMWEKNLNIRT
                     EIIIKDGEEFETAAKNGDFDLIRRGIVLPTTDETTNMLALFSKGVESGKKEDDGGQGI
                     TEIETEKQMPAYKPEETDSTATKDETAESQSAEPPVEPEGDEKTILTEEQALGELPAI
                     PLYFPTSYSLVKPYVQGFETNVLDAPSLKSVRIDNNWQPASRDAKSKK"
     gene            1807..3900
                     /locus_tag="VGB68_11675"
     CDS             1807..3900
                     /locus_tag="VGB68_11675"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY0049941.1"
                     /translation="MARFFQFNQTSLVIALVILSLFISSCSSSADNRFWGKTQAPKDN
                     VLHYISGSEPESLDPALGTGQPEARIYMALYDGLVEYHPKTMEPIPALAESWEVSKDG
                     TEYIFHLRKDGKFSNGAPITAKDFVYTIRRSLKPENAFRNANLAYYIKYAEPYNNGGS
                     FVKDASGRFLLKKDLAEKEPDAAEAAPAAAAPDNFGPDTEFNRFIDEPERLAVPTDEA
                     ERAKLFEKNEKLKAVVEGKELVPVKAEDLGVEAIDDYTLRIKLYQPAPYFLDLLPHQF
                     FRVLHQPTIEKFGKNWVRPENIVTSGAFKVEAHRPYDELIVVKDPQNWDAANVKLERI
                     EFYPLEEATTMMNLYKAGSVDATYNHTTPAAWNEEIRKYKDEYMNHPEVAIEYYSISV
                     KKPPMDNLKVRQAFSLAIDREALAKFRKTTKPLTNFTPEGIFPKYEEARKRVYAEELK
                     KAGISEEDWNRRTFDPERARKLLTEAGFPVQKTATGWTCPTFPADQVQINYNTAESNK
                     AAAEFNQAQWKQNLGIEIPMKNMEWKTFLNYRSKVEYQGFARNGWVGDYMDPFTFLNL
                     FYSPQNDGATGWWDPKYDKMLDDANRETDTTKRFEILARAEFYVISQQIVLPLQTQAT
                     NWIKKPYVKGLYPNPGTLHPWKFVYIERDPARWDKDVDNIMTDKDAAVEEQVGKLMES
                     QKRFEQAKTTANTAE"
BASE COUNT         1108 a         1021 c         1078 g          775 t
ORIGIN      
        1 gaccgatacg gacgcgcgaa ctttagagcc tgttccggcg gttgcggcgc gctggacggc
       61 ttccgaagat tttaaaacct ggacgtttta tctgcgcaaa gacgcgaaat ggtcgaacgg
      121 cgaaaccgtg acggcaaaag atttcgtgcg ttcgtggcag cgcctgactt cgctcggcga
      181 aaaagttcag cagcgcaagc ttttgaaaaa cattatcggg atggacacgg aaaacgtcct
      241 tccggttttt gccggcgaag agattgattt ttccgcgaca tcgccaaatc cggcaagccg
      301 gttgccggcg caaagcctgc aatcgaacag caactcgaac gcggcagcgc ccgcgccgcc
      361 gcaaaaggtc aagcccgacg aaaagaaaac ggaaattccg aaaacggaaa ataaaaccga
      421 atcgaaaacg gcgaaagaag aagaagttaa attcggcgtc gaagccgtcg ataatcacac
      481 tttgaaagtt tcgctcgtcc agcccgacaa ggattttccg gcgctggtcg cgcatccgat
      541 ttttcgcccg atttacggcg acggcaagga attcgaaacc gccgaattaa gcgccaatat
      601 catcaccaac ggcgcgtttc gcattttttc aatcggcagg gacggcgtca cgctcgaccg
      661 cgccgaatat tattggaaca aggcggaaat taatctcgaa cgcgtgcgct ttgtgccgac
      721 cgaaagcgcg gaaatcgctc tggaaggcta tcgcgcgggc gaaatcgacg cgattacgaa
      781 cgcgaatttc gagcctctgg cgctgaagct tttgacgccg tacgacgatt ttcgccgcac
      841 gaaacacagc gcgctgaact tctacgaatt taattcgaaa aatcacccgt tcggcgaccg
      901 ccgcatccgc gaagcgctgg cgattgccat cgagcgcgag cgcctgaccg aagacgaaat
      961 ggacggcgcg accgaaccgg ctttcaatta tttgccgttt gacgagccga gacttatgca
     1021 gaacgtcgaa aaggcgaaaa cgcttctcgc cgaagccgga ttcgccgaaa gcgaaaaatt
     1081 tccggtcgtc aggcttttga ttaatcgcaa cgatttgcag cgccgcatcg cgcgctcggt
     1141 cgccaaaatg tgggaaaaga atctgaacat cagaaccgag attatcatca aggacggcga
     1201 agaatttgaa acggcggcga aaaacggcga ttttgatttg attcgacgcg gcatcgtgct
     1261 gccgaccacc gacgagacga cgaatatgct cgcgctgttt tctaaaggcg tcgaaagcgg
     1321 gaaaaaagaa gacgacggcg ggcagggcat aaccgaaatc gaaaccgaaa aacaaatgcc
     1381 cgcgtataaa ccggaggaaa cggattcgac ggcgacgaaa gatgaaacgg cggaaagcca
     1441 aagcgccgag ccgccggtcg agcccgaagg cgacgaaaaa acgattttga ccgaagaaca
     1501 ggcgcttggc gagctgcccg cgattccgct ttactttccg acttcgtatt cgctggtcaa
     1561 accctacgtg cagggattcg agacgaacgt gctggacgcg ccttcgctga aaagcgtgag
     1621 aatagataat aactggcaac cggcgagccg ggacgcgaaa tctaagaagt aagattgaaa
     1681 ccgaaaaatc gcttgcttgc aatgtttaga acgtgatagc tttatcgcag cggcgcaggt
     1741 tttcaatcgg ctcttcccaa gtcatttata gagtttcagt atttattaaa ataaggagcg
     1801 taagttatgg cgcgattttt tcagtttaac caaacgagtt tggttatagc tctggtcatt
     1861 ttgtctttat ttatatcgag ctgcagcagt tccgccgaca atcgtttctg gggtaaaacg
     1921 caggctccga aagataacgt cctgcattat atttccggct ccgagccgga atcgctcgac
     1981 ccggcgctcg gcaccggaca gcccgaagcg cgcatttata tggctcttta tgacgggctg
     2041 gtcgaatatc atccgaagac gatggaaccg attccggcgc tcgccgaaag ctgggaagta
     2101 agtaaagacg gcacggaata tatttttcac ctgcgcaaag acggcaagtt ctccaacggc
     2161 gcgccgatta ccgcgaaaga tttcgtctac accatccgcc gctcgctcaa acctgaaaac
     2221 gcttttcgca atgccaatct ggcgtattac atcaaatacg ccgagccgta taataacggc
     2281 ggctcatttg taaaagacgc gagcggtcgg tttctgctga aaaaagattt agccgaaaaa
     2341 gaaccggacg cggcggaagc tgcgccggcg gcggctgcgc ccgataattt cggaccggac
     2401 acggaattta atagatttat cgacgagccg gaaagattag ccgtgccgac ggacgaagcg
     2461 gaacgcgcca aattgtttga aaagaacgag aagctgaaag ccgtcgtcga aggcaaggaa
     2521 ctggttccgg tcaaagcgga agatttgggc gtcgaggcga tagacgatta cacgctcaga
     2581 atcaagctct atcagcccgc gccgtatttt ctggatttgc tgccgcatca atttttccgc
     2641 gttctgcacc agccgacaat cgaaaaattc ggtaaaaact gggttcgacc ggaaaacatc
     2701 gtgaccagcg gcgctttcaa agtcgaggcg catcgcccgt atgacgagct tatcgtcgtc
     2761 aaagacccgc agaactggga cgcggcaaac gtcaagctcg aacgcatcga attctacccg
     2821 ctcgaagaag cgacgacgat gatgaatctg tataaagccg gaagcgtcga cgcgacctat
     2881 aatcacacga cgcccgcggc gtggaacgag gaaatccgca aatacaagga tgaatatatg
     2941 aatcatccgg aagtggcgat cgagtattac agcatctcgg tcaaaaagcc gccgatggac
     3001 aacctgaaag tccgtcaggc gttcagcctg gcgatcgacc gcgaagcgct cgccaaattt
     3061 cgcaaaacca cgaagccgct gaccaatttc acgcccgaag gaatttttcc gaaatatgaa
     3121 gaagcgcgga aaagagtcta cgccgaagag ttgaagaaag ccggaatctc ggaagaagac
     3181 tggaaccggc gaacgttcga cccggaacgc gcgcggaagc ttttgaccga agccggtttt
     3241 ccggtgcaga aaaccgccac cggctggacg tgcccgactt ttccggctga ccaggtgcag
     3301 attaattaca acaccgccga atcgaacaaa gccgccgccg aatttaatca ggcgcagtgg
     3361 aagcagaatc tgggaatcga aattccgatg aaaaatatgg agtggaaaac atttctgaat
     3421 taccgctcga aggtcgaata tcagggcttt gcgcgcaacg gctgggtcgg cgattatatg
     3481 gacccgttca cgtttctgaa tcttttctac tcgccgcaga acgacggcgc gaccggctgg
     3541 tgggacccga aatacgacaa gatgctcgac gacgccaacc gggaaaccga tacgacgaag
     3601 cgtttcgaga tactggcgcg cgccgagttt tacgttatct cgcagcaaat cgtgctgccg
     3661 ctccagacgc aggcgaccaa ctggattaaa aagccgtatg tgaaaggctt gtatccgaat
     3721 ccgggaacgc ttcacccctg gaaattcgtc tatatcgagc gcgacccagc cagatgggac
     3781 aaagacgtcg acaacattat gaccgataaa gacgcggcgg tcgaagaaca ggtcggtaaa
     3841 ttaatggaat cgcagaagcg gttcgaacag gcgaaaacaa ccgccaatac agccgaatag
     3901 agtagaggga aaatgttaag tttcattatt cgcagattgt taattatcat tccgatggca
     3961 ttgctggtcg tgacggtcac ct
//
LOCUS       DASWNE010000595         2015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_303387, whole genome shotgun sequence.
ACCESSION   DASWNE010000595 DASWNE010000000
VERSION     DASWNE010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303387"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VGB68_11680"
     CDS             complement(<1..518)
                     /locus_tag="VGB68_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049942.1"
                     /translation="MINVSAQIAVRGETVWTMAGEPITNGVVLIGANGKIEAVGAANQ
                     VRIPQNYKTVSARVVTPGLIDAHSVIGLAGYLNQPHDQMQIDTSNPIQPDLRAFDAYN
                     AEEKLVEWVRGFGVTTLHTGHAPAALVSGQTMIVKTFGKTVDDVVITPTAMIAVTIGD
                     ASLAGQGRSPGTR"
     gene            complement(593..1915)
                     /locus_tag="VGB68_11685"
     CDS             complement(593..1915)
                     /locus_tag="VGB68_11685"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0049943.1"
                     /translation="MKKILVCFAGIFLFACSIFGQEKSTAFINARIIPIVGQPFEQGI
                     LLVQNGKITAVGDARTVRLASNVQIIDLQGKVIMPGLIDTHSHIGEGAGADGSSPIQP
                     DVRLLDSLNARASSLQRAQSGGITTVNVMPGSGHLNSGQTLYLKLRDGNIIDDLLIMT
                     EDGRVAGGMKFANGTNPLRSTPGNFPNTRSKSAAMMREQLVKAQEYREKIRRAGSDKS
                     KMPNRDLVLEAFVEVLDGRRVVHFHTHRHDDILTAIRLSKEFGFRVVLQHVSEAWKVA
                     DEIAKAKVPASIIMIEAPGGKLETMDVSMTNGVALEKAGAAVGFHTDDYITDSRLFLR
                     SAGLAVRNGMSREKALHAMTLAGAQMLDLDKRTGSLETGKDADFIVLSGDPLSVYTHV
                     LQTWVDGKKVFDRSDAKDYLIATGGYGASSDRTFHIDCFDGDVSGGGN"
BASE COUNT          445 a          556 c          550 g          464 t
ORIGIN      
        1 cgcgttccgg gcgagcgtcc ctgaccggcg aggctggcgt cgccgatagt tacggcaatc
       61 atcgccgtcg gcgtaatgac tacatcgtca accgttttgc cgaaagtttt gacaatcatc
      121 gtctgaccgg agacgagcgc ggcgggcgcg tgtccggtgt gcagagtggt gacgccgaaa
      181 ccgcgaaccc actcgacgag cttttcttcc gcgttgtagg cgtcaaaagc gcgcaaatcg
      241 ggctgaatcg ggtttgacgt gtcaatctgc atctggtcgt gcggctggtt cagatagccc
      301 gccagaccga ttacggaatg cgcgtcaatc aaaccgggcg tgacgaccct ggcggagacg
      361 gttttatagt tttggggaat cctgacctga ttcgccgcgc cgacggcttc gattttgccg
      421 tttgcgccga ttaaaacgac gccgttagta atcggctcgc ccgccatcgt ccagacggtt
      481 tcgcctctga cggcgatttg tgccgaaaca tttatcattg aaccttgaac aattaacaat
      541 gcgaacgcga tgaatgataa atggtaaatg atgaatgatg aatgtttttt cattaatttc
      601 cgcctccgga gacgtcgccg tcaaagcaat cgatgtgaaa agtgcggtcg ctgctcgcgc
      661 cgtagccgcc cgtggcgatc agataatctt tcgcgtccga gcggtcgaaa acttttttgc
      721 cgtcgaccca ggtttgcaga acgtgcgtgt aaacgctcaa agggtcgccg gaaagaacga
      781 taaaatcggc gtctttgccg gtttcgagcg agccggttcg tttatccaaa tcgagcatct
      841 gcgcgcccgc cagcgtcatc gcgtggagcg ccttttcgcg cgacataccg ttgcggacgg
      901 cgagtccggc ggagcgcaga aacaggcggg aatcggtgat gtaatcgtcc gtgtgaaagc
      961 cgacagccgc gcccgctttt tccagagcca cgccgttggt catcgaaacg tccatcgttt
     1021 ccagtttccc gccgggcgct tcaatcataa ttatcgacgc cggaactttc gctttggcga
     1081 tttcgtcagc cactttccag gcttcggaaa cgtgctggag aacgacgcga aagccgaatt
     1141 ccttggaaag ccgaatcgcc gtcaaaatgt cgtcgtggcg atgcgtgtga aaatgcacga
     1201 cgcgccttcc gtcgagcact tcgacaaacg cttccaacac caaatcgcgg ttcggcattt
     1261 tcgatttatc gctgccggct cggcggattt tctcgcggta ttcctgcgcc ttgacgagct
     1321 gttcgcgcat catcgcggct gatttcgagc gcgtgttcgg aaaatttccc ggcgtcgaac
     1381 gcagcgggtt cgtgccgttg gcgaatttca ttccgcccgc gacgcgcccg tcttccgtca
     1441 taatcagcaa atcgtcgatg atattgccgt cgcgcaattt taaataaagc gtctgaccgc
     1501 tgttcagatg tcccgaaccc ggcattacat tgacggtcgt aatgccgccg gattgagcgc
     1561 gctgtaaact ggaagcccgc gcattgagcg aatcgagcag gcgcacgtcc ggctgaatcg
     1621 gacttgagcc gtccgcgccc gcgccttcgc cgatgtggct gtgcgtgtcg attaaaccgg
     1681 gcataatgac tttgccctgc aagtcgatta tctgtacatt cgacgcgaga cgaaccgtgc
     1741 gggcgtcgcc gaccgccgtg attttcccgt tttgaaccag cagaattccc tgctcaaacg
     1801 gctgcccgac aatcggaatt attcgcgcgt tgataaaagc cgtcgacttt tcctgaccga
     1861 aaatcgagca ggcaaacaga aaaataccgg caaaacaaac taatattttt ttcattaaat
     1921 atttccccaa gcttttagaa gaatcaggat gtgtaaagtt aaattgcaac gaaaccgtgc
     1981 gcgtgtcaag cgcaaagccg aattacgaat taaaa
//
LOCUS       DASWNE010000596         8209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_268383, whole genome shotgun sequence.
ACCESSION   DASWNE010000596 DASWNE010000000
VERSION     DASWNE010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8209
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268383"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1530
                     /locus_tag="VGB68_11690"
     CDS             <1..1530
                     /locus_tag="VGB68_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049944.1"
                     /translation="LAPTDKTNIGPRLGVAYAFDSRTVIRGGIGLYYAPILYGLNGGG
                     TIKGGTIGYNVDTLFTPNGRNTPANQFLSNFRALSPVDPNSQYVGDLGVVLPFFDKEF
                     KTGRTLQYTVDIQRELPYKFVASIGYIGHRADRLRSNFGRFNALPLDTLKLGNEILNT
                     NINAVTAQQRAYASSIGVTLPANGNAVYTGFNGSVAQALRPFPQYGTNIDNYLESKGE
                     SNYNALQVKFERRFAQGYQFGLSYTFSKLVTNASEDILGGGSALSGVLQNPFDTESLK
                     TISPTNSPHVVVLNFLAELPFGKGKRYLNQGGFVNAIFGGWQVGGIFRYQQGTPLVFA
                     IDQNYSGILQLAGYNGNLRPNLTGQPIGDVRACNQNVAAEVGRRYVLNCLAFAAPPSY
                     SAGPAFILNGQINPAYTQYYSDPLRFFGNAPAVNDEVRSSPYFSENLNVLKKTRLTET
                     ITFEVGAEVFNLFNRVRYLTPDTFLGLPNANGTFNNSNFGAEGATVEPRVVQFRVRVI
                     F"
     gene            1619..3247
                     /locus_tag="VGB68_11695"
     CDS             1619..3247
                     /locus_tag="VGB68_11695"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0049945.1"
                     /translation="MHYKGRKYIGSLILTGCLCCFCPFFVSAQPKTVSLKNQSIEQKL
                     SDARSALAAADLTLAEKLLREALALSPRSVTAHSLAGIVADRKNDLTTAEKHFALAAR
                     LAPKAPETRNNYGAILLRLNRRTEAAREFTASLTANPNQPSALINLGQIRVAENNLTA
                     ARELFEKVKAIAPDIEILRALILISLQLNEKTRAAGEFKEYSAALKSVSVSFAGSKSD
                     EALGKTLLANSLLDEARQEFEYILACDADNINALILLSKVYLQQKNIRAAGKLLESAV
                     ARGIGDAKIYAALADVYQSGGYMENAIPAMRLAIEKEPKNDFYRARYGLLLIDSKAPA
                     AAIIRLNEALKEFPGSARIWLVLGIAQQLDGKSVDARNSFARVLQIEPKSVPALAYMA
                     TSQVEQAQYTEAVKTYERALAVEEKNAILHYLLADTLLKIPTSDAAQIQKHLVRSVEL
                     DEKLAQAHLALGKLYARAENWQKALDEFEQGTRYAPESAEAYYQLGRTLARLKRANES
                     KAAFEKYKKLNETQTAQKEATRQELVRRLANVQF"
     gene            3260..3970
                     /locus_tag="VGB68_11700"
     CDS             3260..3970
                     /locus_tag="VGB68_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049946.1"
                     /translation="MKKSALTIWIIVLTAALGINLPAQTPTPAPQTKAPPQVEEWADD
                     FSGDKLDETKWEKFSFEGGGGGKLEVRDGEVRIRSVAKTRAGIRSAKTFSGDRFIAEA
                     QVAKVGAAYPEPGSRASTIGFAALTILFDGSGRNRIEWILTSEGTFEAWAVTDGRGER
                     LDNRNLGTKMKNPVLSVVRRGDEFLFIINSADGKPEDAQVGLTKTIKNMPKSFHLMLY
                     GFGSSENNWNTARVVVAK"
     gene            4148..5200
                     /locus_tag="VGB68_11705"
     CDS             4148..5200
                     /locus_tag="VGB68_11705"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEY0049947.1"
                     /translation="MFYKHKSRILILMLAALALNAIAFAQPPVGLNRKTPLYQNPVFD
                     FDFPDPTVIRASDGNFYAYATQTVVNGVWRNIQVARSADLKNWQYLGDALPTKPAWAN
                     QTQNFWAPHVSEAGGTYYMYYSADPNAQTGLCLAVATATNPAGPFTDKGAPLKCGGGF
                     VNIDPMAFDDPQTGKKYLYWGSGFEPIKVQELAPNRVDFLSGSQPVNLIFPISDPSPG
                     SYRDLVEGAWVTYRNGYYYLFFSGDDCCTSAHYAVMVSRSTSATGPFTILPENNGVII
                     AGNIHWLGPGHNSVVRDDKSTDWIVYHAYHPEDRPRGRVLLINRLVYGSDGWVRAEIG
                     APTKEVRVAPFINRRR"
     gene            5253..6347
                     /locus_tag="VGB68_11710"
     CDS             5253..6347
                     /locus_tag="VGB68_11710"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEY0049948.1"
                     /translation="MLNFIHKKLFCAALIFLCALMFYTPASAQANVTGEFRNPIFDTD
                     FPDPTVIRASDGYFYAYATQTIVDGKLHHIQAARSKDLINWQRLPDALPEKPVWADKF
                     EPKFWAPHVSEADGKYFMYYSADPNTQKGLCLAVAVSNSPAGPFKDVGKPLQCGESFI
                     NIDPMAFDDPQTGKRLLYWGSGFHPIKVRELAANRIEFAPNSQPVELVATIKDENPDN
                     YQRLVEGAWVTYRKGYYYLFYSGDNCCGDKAHYAVMVARSKSATGPFETLAQAAAGRK
                     YSVILEMNDEWLAPGHNSVIRDDKGQDWIFYHAINVKNRNLSKAESIGGDRGDRRVML
                     MNRLVYKNGWVRAEGGTPALGKQKAPKIRK"
     gene            6354..6530
                     /locus_tag="VGB68_11715"
     CDS             6354..6530
                     /locus_tag="VGB68_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049949.1"
                     /translation="MEDEGKTPDTGVGKMRKRREAMPDGKRYIIYYTFGEVENSEPMI
                     REKSEEKREESENV"
     gene            6523..7515
                     /gene="galT"
                     /locus_tag="VGB68_11720"
     CDS             6523..7515
                     /gene="galT"
                     /locus_tag="VGB68_11720"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEY0049950.1"
                     /translation="MSELRWHPLLGEWVATATHRQDRTFLPPADFCPLCPTKPGGFPT
                     EIPESIYDIVVFENRFPSLSPNPPEPAIEGDDFYPVRPAFGECEVVVYTPNHDATLAQ
                     EPVEQIYKLVQVWTDRFRDLSALEFVKYVFIFENKGKDIGVTLHHPHGQIYAYPFVPP
                     RVLREIEQSKKHLAETGNCLLCDILKKEKTDARRIVAENESFAAYIPFFARYPYELHI
                     SAKRHLLDLTEFTAREQIDLAEILKQVLVAFDKLFNIPFPYMMVLHQKPSDGESYDFY
                     HFHIEFYPPLRTATKLKYLAGSETGAGLFINDTLAEEKAAELRALVETVVWTKQ"
     gene            7512..>8209
                     /gene="galK"
                     /locus_tag="VGB68_11725"
     CDS             7512..>8209
                     /gene="galK"
                     /locus_tag="VGB68_11725"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEY0049951.1"
                     /translation="MIDPQRLKEKFTEFYDGEPRLFRAPGRVNLIGEHTDYNGGFVLP
                     MAIDKEAAVAISERADRKIRVYTVNLDEAAEFDLDRGNDRKKGFWLNYIEGVARILES
                     KNVRLKGADLIIWSDVPTGAGLSSSAALEMVTGLALSEISNHTVDRVTLAKIGQQTEH
                     DYVGAKVGIMDQFVSAHARARHALLLDCRSLEFENVPLDTREVAVVICDTNVKHDLAT
                     SGYNTRREECEQAVR"
BASE COUNT         1958 a         2129 c         2291 g         1831 t
ORIGIN      
        1 ctggcgccga cggataaaac aaatatcgga ccgcgcctcg gcgtggctta cgcgttcgac
       61 agccgaaccg tcattcgcgg cggcatcggt ttgtattacg cgccgattct ttacggttta
      121 aacggcggcg gcaccatcaa aggcggcacc atcggctata acgtcgatac gcttttcacg
      181 ccgaacggtc gaaatactcc ggcaaatcag tttttatcca atttccgcgc gctttcgccg
      241 gtcgacccga acagccaata cgtcggcgat ttgggcgtcg tgcttccgtt ttttgataaa
      301 gagttcaaga ccggacgaac gctgcaatat accgtcgata ttcagcgcga gctgccgtat
      361 aaattcgtcg cctcgattgg atatatcggg catcgcgccg accgcctgcg ctcgaatttc
      421 gggcggttta acgcgcttcc gctggatact ctgaagctcg gcaatgaaat tctgaacacg
      481 aacatcaacg ccgtcaccgc gcagcagagg gcttatgcgt cgagcatcgg cgtcacgctt
      541 ccggcaaacg gaaatgccgt ttatacgggc tttaacggca gcgtcgcgca ggctttgaga
      601 ccgttcccgc aatacggaac gaacatcgac aattatctgg aaagcaaggg cgaaagcaac
      661 tataacgcgc ttcaggttaa attcgagcgt cgtttcgcgc agggctatca attcggcttg
      721 tcctatactt tctccaagct tgtcaccaac gcttcggaag acattctcgg cggcggttcg
      781 gctctgagcg gcgtcttgca gaatccgttt gacaccgaat ccttaaagac gatttcgccg
      841 accaattcgc cgcacgtcgt ggtgcttaat ttcctggcgg agctgccttt cggcaagggc
      901 aagcgttacc tgaatcaggg cggcttcgtc aacgccatct tcggcggctg gcaggtcggc
      961 ggaattttcc gctaccagca gggaacgccg ctcgtttttg ccatcgacca gaattacagc
     1021 ggcattttgc agcttgcagg ttacaacgga aatctgcgtc cgaatctgac gggacagcct
     1081 atcggcgacg tcagagcctg taatcagaac gtcgccgccg aagtcggcag gcgctatgtt
     1141 ctgaattgtc tggcgtttgc cgctccgccg agctactcgg caggaccggc gtttatcctg
     1201 aacggacaaa tcaatccggc ttacacgcag tattattccg acccgctcag atttttcgga
     1261 aacgcgccgg cggttaatga tgaagttcgt tcatcgccgt atttttcgga aaatctgaac
     1321 gttttgaaga aaacgcgtct gactgaaacg attaccttcg aggtcggcgc ggaagtcttc
     1381 aatttgttta accgcgttcg ctatctgacg cccgacacat tcctcggctt gccgaacgcc
     1441 aacggaacgt ttaacaactc gaacttcggc gcggaaggcg cgaccgtcga gccgcgcgtc
     1501 gtccaattcc gggtcagagt tattttttag ccacggctcg taatctgcgg cgtgagaata
     1561 tgtgttataa aaaacatatt ctcacgctgt ttaatctttc cgacatcctg gtttattgat
     1621 gcattataag gggcgaaaat acatcggcag tttaattttg acgggctgtt tgtgttgttt
     1681 ttgtcctttt ttcgtctccg cgcagccgaa aaccgtaagt ttaaaaaatc aatcaatcga
     1741 gcaaaaatta tccgacgccc gcagcgctct ggcggcggcg gatttgacgc ttgccgaaaa
     1801 gcttctgcgc gaagctctgg cgctttcgcc gcgcagcgtc acggcgcaca gcctcgcggg
     1861 aatcgtcgcc gaccgcaaaa acgatttgac gacggcggaa aaacatttcg cgctcgccgc
     1921 ccggctcgcc ccgaaagcgc ccgaaacgcg caataattac ggcgcgattc tgctccgtct
     1981 aaatcgccga accgaagccg ccagagaatt taccgcgtcc ttaacggcaa atccgaacca
     2041 gccgagcgcg ctcatcaatc tcggacaaat ccgcgtggcg gaaaataatc tgacggcggc
     2101 gcgcgaatta ttcgaaaaag tcaaagcaat cgcgcccgac atcgaaattc tgcgcgccct
     2161 gattttgatt tccctgcaat taaacgaaaa aactcgcgct gccggggaat ttaaggaata
     2221 ctcagccgca ttaaaaagcg tttccgtttc gtttgccggc tcgaaatcgg acgaagcgct
     2281 gggaaagaca ctgctggcaa acagtctgct cgacgaagcg cggcaggaat ttgaatatat
     2341 tctcgcctgc gacgccgata atattaacgc gctgatttta ttgtcgaagg tttatttgca
     2401 gcagaaaaat attcgcgccg ccggaaaact gctcgaatcg gcggtcgctc gcggcatcgg
     2461 cgacgcgaaa atatacgccg cgctagccga cgtttatcaa agcggcggct atatggaaaa
     2521 cgcgattccg gcgatgcgtc tggcgattga aaaagagccg aaaaacgatt tttaccgcgc
     2581 ccgctacggc ttgcttttga ttgactcgaa agccccggcg gcggcgataa tccggctcaa
     2641 tgaagcgctc aaagaatttc cgggttcggc gcgcatctgg ctcgtgctcg gcatcgcgca
     2701 gcaattggac ggcaaatccg tcgacgcccg aaattctttt gcgcgagttt tgcaaatcga
     2761 gccgaaatcc gttccggcgc tggcgtatat ggcgacttcg caggtcgagc aggcgcagta
     2821 tacggaagcg gtgaaaactt acgagcgtgc gcttgccgtc gaggaaaaaa acgctattct
     2881 gcattatttg ctcgccgata cgcttttgaa aattccgacc agcgacgcgg cgcagattca
     2941 gaaacattta gtgcgctcgg tcgagcttga cgaaaagctg gcgcaggcgc acctcgcgct
     3001 cggaaagctc tatgcccgcg ccgaaaactg gcaaaaagcg ctcgacgagt ttgagcaggg
     3061 cacgcgctac gcgccggaat cagccgaagc ttattaccag ctcggtcgca cgctggcacg
     3121 gcttaaacgc gccaacgaat cgaaagcggc ttttgaaaaa tataaaaaat taaacgaaac
     3181 gcagaccgcg cagaaagaag ccacgcggca ggaactcgtc cggcgtctgg caaatgtgca
     3241 gttttaaaaa aggggtttta tgaaaaagtc agccttaaca atttggataa tcgttttaac
     3301 cgcagccctg ggaatcaatt tgccggcgca gacgccgacg cccgcgccgc agacgaaagc
     3361 gccgccgcag gtcgaagaat gggcggatga tttttccggc gataagctgg acgaaacgaa
     3421 gtgggaaaaa ttttcctttg aaggcggcgg cggcggcaag ctcgaagtca gggacggcga
     3481 agtccgcatt cgcagcgtcg ccaaaacgcg cgccggaatc cgcagcgcga aaactttcag
     3541 cggcgaccgc tttatcgccg aagcccaggt tgccaaagtc ggcgcggcgt atcccgaacc
     3601 gggcagcagg gcttcgacca tcggtttcgc cgcgttgacg attctttttg acgggtcggg
     3661 acgcaaccgc atcgaatgga ttttgacgag cgaaggcacg tttgaagcgt gggcggtgac
     3721 ggacggtcgc ggcgagcggc tcgacaatcg caatctcggc acgaaaatga aaaatccggt
     3781 tttgtccgtc gtccggcgcg gcgacgaatt tttattcatc atcaacagcg ccgacggtaa
     3841 gcccgaagac gcgcaggtcg ggctgaccaa gacgattaaa aatatgccga agtcttttca
     3901 tctgatgctt tacggtttcg gctcgtcgga aaataactgg aacacggcgc gcgtcgtcgt
     3961 ggcgaaataa attttaatga tttaatggaa aagaaatttg aaaatcggac ggtttctttt
     4021 cgctcttcgc cgcttcttcg cgacctctgc gataaaaaaa tcttaaccgc aaagcgcgca
     4081 aaggtttccg gctctttatc gacactgtta gtcttttgca atcgttaagg tcgggaataa
     4141 gaataatatg ttttataaac acaaatcccg aattttgatt ctgatgttag cggctttggc
     4201 gctgaatgcg attgcctttg cgcagccgcc ggtcggtctg aacagaaaaa cgccgcttta
     4261 tcaaaatccg gtttttgatt tcgactttcc cgacccgacc gtcattcgcg cttcggacgg
     4321 caatttttac gcctatgcga cgcaaaccgt cgtcaacggc gtctggcgaa acatacaggt
     4381 cgcgcgctcg gcggatttga aaaactggca gtatctcggc gacgctttgc cgaccaagcc
     4441 tgcctgggcg aaccagacgc agaatttttg ggctccgcac gtttcggaag cgggcggcac
     4501 gtattatatg tattattccg ccgacccgaa cgcgcagacc ggactttgtc tggcggtggc
     4561 gacggcgacc aacccggcag gaccttttac ggataaaggc gcgccgctga aatgcggcgg
     4621 cggcttcgtc aacatcgacc cgatggcgtt tgacgacccg cagacgggaa aaaaatatct
     4681 ttactggggc agcggcttcg agccgataaa agtgcaggag ctcgcgccga atcgcgtcga
     4741 ttttttgtcc ggctcgcagc ccgttaatct gatatttcca atcagcgacc cgagtcccgg
     4801 cagttatcgc gatttggtcg aaggggcgtg ggtgacttat cgcaacggct attattattt
     4861 gtttttttca ggcgatgatt gctgcaccag cgcgcattac gccgtgatgg tctcgcgctc
     4921 gaccagcgcg accggacctt ttacaatttt gccggaaaat aacggcgtca taattgcagg
     4981 aaatattcac tggctcggtc ccggtcacaa ttccgtcgtt cgcgacgata aatcgaccga
     5041 ttggattgtc tatcacgcgt atcacccgga agaccgaccg cgagggcgcg tgttgctgat
     5101 aaatcgcctg gtttacggca gcgacggctg ggttcgggcg gaaatcggcg cgccgacaaa
     5161 agaagttcgg gtcgcgccgt ttatcaatcg gcgaagataa cgcgtcgggc gcgtttacag
     5221 attaattgcg ggatttttta ttgcggttag ggatgcttaa ttttattcac aaaaaattgt
     5281 tttgcgcggc attgattttc ctgtgcgcgc tgatgtttta tacgccggcg tcggcgcagg
     5341 cgaatgttac gggcgagttc cggaatccga ttttcgacac ggattttccc gacccgaccg
     5401 tcattcgcgc ttccgacggg tatttttacg cctacgcgac gcagacgatt gttgacggga
     5461 aactccatca catacaggcg gcgcgttcga aagatttgat taactggcag cgcctgccgg
     5521 acgctctgcc ggaaaagccc gtctgggcgg acaaattcga gccgaaattc tgggcgccgc
     5581 acgtctcgga agccgacgga aaatatttta tgtattattc ggctgacccg aacacgcaga
     5641 aaggcttgtg tctggcggtc gccgtttcaa attcgcccgc cggacctttc aaggatgtcg
     5701 gcaagcctct gcaatgcggc gaaagcttta taaacatcga cccgatggcg tttgacgacc
     5761 cgcagaccgg aaaacggctt ttgtattggg gaagcggttt tcacccgata aaagttcggg
     5821 agctggcggc gaatcgaatc gaatttgcgc cgaacagcca gccggtcgag ctggtggcga
     5881 cgattaaaga cgaaaatccc gataattacc agcgtcttgt cgaaggcgct tgggtaacgt
     5941 atcgcaaggg ctattattat ttattttatt cgggcgataa ctgctgcggc gacaaggcgc
     6001 attacgccgt gatggtagcg cgctcaaaaa gcgcgaccgg acctttcgag actctggcgc
     6061 aggctgcggc gggcagaaaa tacagcgtga ttttagagat gaacgacgaa tggctggcgc
     6121 ccggtcataa ttcggtgatt cgcgacgata agggtcagga ctggattttt tatcacgcca
     6181 tcaacgtgaa aaaccgcaac ctgtcgaaag cggaaagcat cggcggcgac cgcggcgacc
     6241 ggcgcgtgat gctgatgaat cgtctggttt ataaaaacgg atgggtccgg gcggaaggcg
     6301 gaacgcccgc gctcggaaaa caaaaagcgc ctaaaatccg caaatagttg aatatggaag
     6361 acgagggaaa aacgccggac acaggcgtcg gcaaaatgcg taagcgtcgt gaagcgatgc
     6421 cggacggaaa gcgttatatt atttattata cttttggaga ggtcgaaaat tccgagccga
     6481 tgatacggga aaaatcggaa gaaaagcggg aggaaagcga aaatgtctga gcttcgatgg
     6541 catccgcttt tgggcgaatg ggtggcgacg gcgacacacc gacaggacag aacttttctg
     6601 ccgcccgccg atttttgccc gctctgcccg accaagccgg gcggttttcc gacggaaatt
     6661 cccgaaagca tttacgatat tgtcgtcttt gaaaaccgtt ttccgtcgct ttcgcccaat
     6721 ccgcccgaac cggcgattga aggcgacgat ttttatcccg ttcgaccggc tttcggcgag
     6781 tgtgaggtgg tcgtttacac gcccaatcac gatgcgacgc tggcgcagga gccggtcgag
     6841 cagatttata aactggtgca ggtttggacg gacaggtttc gggatttatc ggcgctcgaa
     6901 ttcgtcaaat acgtttttat ctttgaaaat aaaggcaagg acatcggcgt cacgctgcat
     6961 cacccgcacg ggcagattta cgcttacccg tttgtgccgc cgcgcgttct gcgcgagatt
     7021 gagcaatcga aaaagcatct cgcggaaacg ggaaattgct tattgtgcga tattttaaaa
     7081 aaagaaaaga cggacgcgcg gcgaatcgtc gccgaaaacg aaagctttgc cgcttacatc
     7141 ccgttttttg cgcgctaccc gtatgagctg cacatctcgg caaagcggca tttgctggat
     7201 ttgacggaat ttacggcgcg cgagcagatt gatttggcgg aaattttaaa acaggttctc
     7261 gtcgcttttg ataaactttt taatattccg tttccctata tgatggtctt gcatcagaag
     7321 ccttcggacg gcgaaagtta cgatttttat cattttcaca tcgagtttta cccgcccttg
     7381 aggacggcga cgaaactgaa atatctggcg ggcagcgaga cgggcgcggg attgtttatc
     7441 aacgacacgc tggcggaaga aaaagccgcc gagctgcgcg cgctggtaga aacggttgtc
     7501 tggacgaaac aatgattgac ccgcaaagat taaaagaaaa gtttacagag ttttacgacg
     7561 gcgagccgcg actttttcgc gcgcccggca gagtcaatct aatcggcgag cataccgact
     7621 ataacggcgg attcgttctg ccgatggcga ttgataaaga agcggcggtc gccattagtg
     7681 aaagagcaga ccgcaaaatc cgcgtctaca cggtcaatct cgacgaagcg gcggaatttg
     7741 atttagaccg cgggaatgat agaaaaaaag gtttctggct gaattatatc gagggcgtcg
     7801 cgcggattct ggaaagcaaa aacgtgcgtt tgaagggcgc ggatttaatc atctggtcgg
     7861 acgtgccgac gggcgcgggt ctttcgtctt cggcggcgct ggaaatggtg acgggtttgg
     7921 ctctgagcga gatttcgaat cacacggtcg accgcgtcac gctggcgaaa atcgggcagc
     7981 agaccgagca cgattacgtc ggcgcgaaag tcgggattat ggaccagttc gtttcggctc
     8041 acgcccgtgc gcggcacgcg cttctgctcg attgccgcag tctggaattt gaaaacgtcc
     8101 cgctcgacac gcgcgaagtc gcggtcgtga tatgcgatac gaacgtgaaa cacgatttgg
     8161 cgacatcggg ctataacact cggcgcgaag aatgcgagca agccgtgcg
//
LOCUS       DASWNE010000597         1905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_303408, whole genome shotgun sequence.
ACCESSION   DASWNE010000597 DASWNE010000000
VERSION     DASWNE010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303408"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VGB68_11730"
     CDS             complement(<1..574)
                     /locus_tag="VGB68_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049952.1"
                     /translation="MSFINSSNQLYGANRRGELNFEAAFGGALMPRIYSPNVSGELSF
                     EATFKGEFRPQVGGSRRLNLRVKSSGEITPRVRLYLSEPPPILNEISPTAFQNQKLKA
                     RLLVNGVETKYKSFNYSKPRGASGASLTIEMARADLSLLPEGASFTFQIGKKVSGVYE
                     WTSLVENAVLDARSYAIAYLNDRLSFSTVAP"
     gene            complement(578..1561)
                     /locus_tag="VGB68_11735"
     CDS             complement(578..1561)
                     /locus_tag="VGB68_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049953.1"
                     /translation="MINSLTLIYTGGNLSGATSGVIYVPIMKDETIVSLDVKADENVS
                     GANAVFNLFLNGVEIEDLAPTINIGSDIVTISDLEIEIEKGDELVLNLVSGSVSSPVT
                     LNLDVDDGNSGGIASAAYGVDVPPASPTAQDEEFDDADNVNALYTITAGSGVTYDVNT
                     TIPSHLYAYFPGVSKTLHLEFPYAPVGDFSLTAKVSFQCRTASSFAGFIISAYSADEQ
                     DAIRAFWQNNTNPIVGLDYQDGGSWSFFAINETVLANLTGMVIHLQRVSDSWTAWFSL
                     DGGRTFLRAGSSFHNKSLSVTHVEIDFQTGTQNEKVVGTIDWIRRGWRLFT"
BASE COUNT          488 a          450 c          504 g          463 t
ORIGIN      
        1 gcggcgcgac ggtcgaaaac gaaagccggt cgttcagata ggcgatggca tagcttctgg
       61 catcgagtac cgcgttttcc actaatgatg tccactcgta aacgccggag acttttttgc
      121 cgatttgaaa cgtgaaactc gcgccttcag gcaaaagcga taaatcggcg cgtgccattt
      181 cgatggtgag cgaagcgccg gatgcgccgc gcggcttgct gtaattgaaa gatttgtatt
      241 tggtttcgac gccgttgacc aaaaggcgag cttttaattt ttgattctga aaagcggtcg
      301 ggctgatttc gtttaaaatc ggcggcggct cggacaggta aagccggacg cgaggagtga
      361 tttcgccgga ggatttgacg cgcagattca gacggcggct gccgccgact tgcggacgga
      421 attcgccttt gaaagtcgct tcaaaagata attcgccgga aacgttcggc gaataaattc
      481 gcggcataag agcgccgccg aaggcagctt cgaagttcaa ttcaccgcgc cggttcgcgc
      541 cgtaaagctg gttggatgaa ttgatgaagc tcatttatca ggtgaataaa cgccagccgc
      601 gcctaatcca gtcgattgta ccgacgactt tttcgttttg cgtgccggtt tgaaaatcta
      661 tttcgacgtg agtaaccgaa aggcttttgt tgtgaaagct cgaaccggcg cgcagaaaag
      721 tgcgcccgcc gtcgagcgaa aaccacgccg tccacgaatc cgagactctc tgcaaatgaa
      781 taaccatccc ggtcaaattg gcgagcaccg tttcgttgat ggcgaagaaa gaccagctgc
      841 cgccgtcctg gtaatcgagt ccgacaatcg gatttgtgtt gttctgccag aaggcacgaa
      901 tggcgtcctg ctcgtcggcg ctgtacgcgc tgatgataaa accggcgaag ctcgatgcgg
      961 tgcggcactg aaagctgact ttcgccgtca gcgaaaaatc gccgaccggc gcgtaaggaa
     1021 actcaagatg cagcgttttg ctgacgccgg gaaaataagc gtagagatgc gaaggaatgg
     1081 tcgtattgac atcgtaagtg acgcctgagc cggcagtgat ggtataaagc gcgttgacat
     1141 tatccgcatc gtcgaactct tcgtcctgtg ccgtcggcga cgcgggcgga acgtccacgc
     1201 cgtaagccgc tgaagcgatg ccgccggaat tgccgtcgtc gacgtcgaga ttcagtgtga
     1261 ccgggctgga aacgctgccc gaaacgagat tcaaaacaag ctcgtcgcct ttctcaatct
     1321 cgatttccaa atcggaaatc gtcacgatgt ccgaaccgat attgatggtc ggcgctaaat
     1381 cttcaatctc gacgccgttc aaaaataaat tgaaaacggc atttgcgccc gaaacgttct
     1441 cgtcggcttt gacgtcgaga ctgacaatcg tttcgtcctt cataatcggc acgtagataa
     1501 cgccgctcgt cgcgccggaa agattgccgc cggtgtaaat taaggtgagt gaattaatca
     1561 ttgccgttta ctcaagcgag agtgtcgcta cagaacttca aataaaagtt taactaacga
     1621 tgaaaggtag ctgttcagat tctcgaaaag tgaaaagctg agtttttcac ttagtcttta
     1681 cagtttataa aattatttga aagcggtcgg taagaggttt aatgaataaa ttaactttat
     1741 cagcacagta tttcggcaat aaaaaagagg ctcgtttaaa gcctcttttt tattgcctgt
     1801 taaacttaac tttgctggaa taactatttt tcgagtgata tgttgtcagg cgtaaatgtc
     1861 aacaaatcgc tcggctcaca ttttagcgca ttgcataaca cattc
//
LOCUS       DASWNE010000598         3812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_443395, whole genome shotgun sequence.
ACCESSION   DASWNE010000598 DASWNE010000000
VERSION     DASWNE010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443395"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2723)
                     /locus_tag="VGB68_11740"
     CDS             complement(<1..2723)
                     /locus_tag="VGB68_11740"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0049954.1"
                     /translation="MTPEQWEQISEIYHSALEIDSSERRKAFLEKMCANDEALRGEVE
                     SLLAANSELGDFIAEPVVRDIAPLLKVENAPPISLAGKSLGHYRIVSRIGFGGMGEVY
                     LAKDSKLNRAVAVKTLPASFSKDANHLRRFQTEAKAAATLNHPNVATIYSVEEIDSQP
                     FITMEYVEGKSLDTFIPAGGLDLKTFLEWFIPLADALSHAHEKGIIHRDIKPGNIMIT
                     AEGVPKILDFGLAQIDKAVTSAASAEDSSTLDMTEPGQIFGTPSYMSPEQAEGRQVDY
                     RSDIFSFGVVMYQALAGARPFKGDSYAAIVGELMNKEPRPVGEIKTETPFLLSRLVMR
                     CLCKERRKRFQSMREVRAILEETKAAVEAGGSPKSSSLRFLKSKSKASRRGWFLAPAA
                     LLAIIAASFALYRFFQDKPQPPISFENMTLRKLSQTNNVAFAQITPDAKSIVYTTIDE
                     NDGRALWIRRVDDKNALQLLPSQPVFFWGGLTVSADGSQIYYIVAERDGLRRALYRVS
                     SLGGAPRKLVETVNDLGSLSPDGQRILYVRYGEKQVQILSANASDGGDERVIRSGKDN
                     EIFRDPRFSLDGKSIFFIKFERIDGEEFWSLVEMPSAGGDERVILPLRKPRISEIVVL
                     KDGSGLLVNAIDAVSNLQQIFYVSLPSGLETRITNDLNAYFGISASDDGKTIVATQRQ
                     NLRDIWLAPEGGGDNLRKLTSESNVYTSAVWTADGRIVYDAVDNNRPHIWIMNGDGTG
                     AQQLTPNDSFDYEPRVSPDGRHIVFTSKRGGETKIWRMNIDGSNPQILTTVGGATFAP
                     VVTPDGQTVLFQWSKENGRVMGSVALAGGAAAEMTPLSPNSNLLAISPDGRQVAFPFY
                     DQQSARYKVCVRPLETAEPSVVFDITPTTFLQWTFDGKGLLYRELDPRRNPG"
     gene            complement(2808..3383)
                     /locus_tag="VGB68_11745"
     CDS             complement(2808..3383)
                     /locus_tag="VGB68_11745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0049955.1"
                     /translation="MNQPASQEITRLLLAWGSGDKAALDALMPIVYDELRRLAKRYMR
                     RQNPDHTLQTTALVNEAYLRLIDSSRVRWQDRTHFFAISAQLMRRILVDFARAKNSRK
                     RGGEQIQITLDERIEAPFETETNLVELDEALQRLAKLSPRQSRIVELRYFGGLSEEEI
                     SETLAISTRTVRRDWSLARAWLYRELNNNDK"
     gene            3594..>3812
                     /locus_tag="VGB68_11750"
     CDS             3594..>3812
                     /locus_tag="VGB68_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049956.1"
                     /translation="MPKVETIEGFQVINVSASVGKNAVNLKDDVIVVQALLKYVFETG
                     DFFKGVVLPEPTGTMDKTTAWVIKRYQQL"
BASE COUNT          808 a         1075 c         1058 g          871 t
ORIGIN      
        1 cctgggttgc gcctcgggtc taattcccgg taaagcagac ctttgccgtc gaatgtccat
       61 tgcagaaatg tcgtcggcgt aatgtcgaaa acaaccgacg gctctgcggt ttcgagcggg
      121 cgaacgcaga ctttataacg cgcgctctgc tggtcgtaaa aaggaaaggc gacctgtctg
      181 ccgtcgggcg agatggcgag aagattcgag ttcgggctca acggcgtcat ctcggcagcc
      241 gcgccgcccg ccagcgcaac gcttcccatc acgcgcccgt tttctttcga ccattgaaac
      301 aaaaccgtct gcccgtcggg cgtcacgacg ggcgcaaatg tcgcgccgcc gaccgtcgtt
      361 aaaatctgcg gattgctgcc gtcgatgttc atccgccaga ttttcgtctc gccgccgcgc
      421 ttcgaggtaa aaacgatatg gcgaccgtcg ggcgagacgc gcggctcgta gtcgaacgag
      481 tcgttcggcg tcagttgctg cgcgccggtg ccgtcgccgt tcataatcca tatgtgcgga
      541 cggttattgt ccaccgcgtc gtaaacgatg cgcccgtcag ccgtccagac ggctgacgta
      601 taaacgttcg attcgctcgt caatttacga agattgtcgc cgccgccttc gggcgcgagc
      661 cagatgtccc tgagattctg tctctgcgtg gcgacgatgg ttttgccgtc gtcgctggcg
      721 ctgatgccga aatacgcgtt caaatcgttg gtgatgcgcg tttccagacc gctcggcagc
      781 gaaacgtaaa aaatctgctg gagattggaa accgcgtcga tcgcattgac cagcaaaccg
      841 cttccgtctt tcagcacgac gatttcgctg attctcggtt tgcgcagcgg gagaataact
      901 ctttcgtcgc cgcccgccga cggcatctcg accagcgacc agaattcttc gccgtcgatt
      961 ctttcgaact tgatgaaaaa aatgcttttg ccgtcgagcg aaaaccgcgg gtcgcggaag
     1021 atttcgttgt ctttgccgct gcgaatcacg cgttcgtcgc cgccgtcaga cgcgttcgcc
     1081 gaaagaatct gcacctgttt ttcgccgtag cgaacgtaaa gaatacgctg tccgtcgggc
     1141 gagagagagc ctaaatcgtt gaccgtctcg accagcttgc gcggcgcgcc gccgagcgac
     1201 gagacccggt agagcgctcg gcgcagcccg tcgcgttcgg cgacgatgta ataaatctgg
     1261 ctgccgtcgg ctgagaccgt cagcccgccc cagaaaaaca cgggctgcga aggaagcagc
     1321 tgaagcgcgt ttttgtcgtc gacgcggcga atccagagcg cgcgcccgtc attttcgtcg
     1381 attgtggtgt agacgattga tttggcgtcc ggcgtgattt gagcgaaagc gacgttgttt
     1441 gtctgcgaga gtttccgcag cgtcatattc tcaaaactta tcggcggctg cggcttgtcc
     1501 tgaaaaaaac gataaagcgc gaaagacgcg gcgatgattg ccagcagagc cgccggcgcg
     1561 agaaaccagc cgcgccggga agctttcgat ttcgatttta aaaaccgcag cgatgaactc
     1621 ttcggcgacc cgcccgcttc gaccgccgct ttcgtttctt cgagaatcgc gcggacttcg
     1681 cgcatcgatt gaaagcgttt gcggcgctct ttgcacagac agcgcatcac gaggcgcgag
     1741 agcagaaaag gcgtttcggt tttgatttcg ccgacggggc gcggctcttt gttcatcagc
     1801 tcgccgacaa tagcggcgta gctgtcgcct ttaaacggtc gcgcgcccgc gagcgcctga
     1861 tacatcacga cgccgaagct gaaaatatcg ctccggtaat cgacctgcct gccttcggct
     1921 tgttcgggcg acatatagga aggcgtgccg aaaatctgtc ccggctcggt catatccagc
     1981 gtcgacgaat cttcagccga cgccgccgag gtgacggctt tatcaatctg cgccaagccg
     2041 aaatccagaa ttttcggcac gccttcagcc gtaatcataa tgttgcccgg cttgatgtcg
     2101 cgatgaataa tgcctttttc gtgcgcgtgc gagagcgcgt cggcgagcgg gataaaccat
     2161 tcgagaaacg ttttcaaatc gagcccgccc gccggaataa acgtgtccag agattttcct
     2221 tcgacgtatt ccatcgtgat aaacggctgg ctgtcgattt cctcgaccga ataaatcgtg
     2281 gcgacattag gatgattaag cgtcgcggcg gctttcgctt ccgtttgaaa acggcgcagg
     2341 tgattcgcat ccttcgaaaa cgaagcgggc agagtcttga cggcgaccgc gcgattgagc
     2401 tttgaatctt tcgccagata aacctcgccc attcccccga agccgatgcg cgagacaatc
     2461 cggtaatgac cgaggctttt tcccgctaac gaaatcggcg gcgcgttttc gaccttcaat
     2521 aaaggcgcga tgtctctgac gaccggctcg gcaataaagt cgccgagctc ggaatttgcg
     2581 gcgagcagcg attcgacttc gccgcgcagg gcttcatcat tcgcgcacat cttttcgaga
     2641 aacgcttttc gccgttcgct cgaatcgatc tccagcgcgg aatgataaat ttcgctgatt
     2701 tgttcccatt gttcgggagt cataaagcaa atcaaccacg gatgtactcg gattaactct
     2761 gattattttt agattttatc cgaatgcagc cgtgaaaatc cgtaatttca tttatcgtta
     2821 ttattcagct cgcgatagag ccaggcgcgc gccagactcc agtcgcggcg cacggtgcgc
     2881 gtcgagatag caagcgtttc actgatttct tcttcgctca agccgccgaa gtatcgcagc
     2941 tcgacaatcc ggctctggcg cgggctcagt tttgcgagcc tttgcaaagc ttcgtccagc
     3001 tcgaccagat tcgtctccgt ttcaaaaggc gcttcgattc gctcgtcgag cgtgatttgt
     3061 atctgctcgc cgccgcgttt ccggctgttt ttggcgcggg caaaatcgac cagaatacgc
     3121 cgcatcaact gcgccgaaat cgcaaaaaaa tgcgttctgt cctgccagcg cacgcggctc
     3181 gaatcaatca ggcgcagata agcttcattg accagcgccg tcgtttgcag cgtgtggtcg
     3241 ggattctggc ggcgcatata acgctttgcc agccggcgca gctcgtcgta gacgatcggc
     3301 atcagcgcgt ccagagccgc tttgtcgccg cttccccagg cgagaagcag ccgcgttatt
     3361 tcttgtgatg caggctggtt catctcggaa aattcacagc gcccggagcg ccgattcggc
     3421 attagcttac cacaactgtt taacgggcaa aaataaaaat tacgcgcgcc ttttgtgtcc
     3481 tgtttccgcc gccgattgcg tcttcaatgg cgagagctcg ggcaagttct cgcaaagttc
     3541 atctttcccg atttttcggg ataaaaattt caataaatat aaggagcaat tatatgccaa
     3601 aagtagaaac catcgagggc tttcaggtca tcaacgtgtc ggcttcggtc ggcaaaaacg
     3661 ccgtcaatct aaaggacgac gtgatagtcg tgcaggcgct gctgaaatat gttttcgaga
     3721 cgggcgattt ttttaaaggc gtcgtgctgc cggagccgac cggaacaatg gacaaaacga
     3781 cggcgtgggt tattaaaaga taccagcagc tg
//
LOCUS       DASWNE010000599         2581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_124812, whole genome shotgun sequence.
ACCESSION   DASWNE010000599 DASWNE010000000
VERSION     DASWNE010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124812"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VGB68_11755"
     CDS             complement(<1..441)
                     /locus_tag="VGB68_11755"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0049957.1"
                     /translation="MKLYYESHGAGEPLILIPGFASGAWAWFEQIEELSKTFRVITFD
                     PCGVSRSATDEKLTEAISLPTIAADVARLLDFLQIEKANVLGVSFGGFVAQEFALGFP
                     ERLEKLILACTSFGGKNHVAPDLEVLAAFVAADDLNKQERVRRFM"
     gene            complement(543..1487)
                     /locus_tag="VGB68_11760"
     CDS             complement(543..1487)
                     /locus_tag="VGB68_11760"
                     /inference="COORDINATES: protein motif:HMM:NF013810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HEY0049958.1"
                     /translation="MNRKKSFIQACSVLILVLISAQAASAQLTTHFQNWLSANGYDGY
                     NFNYGGNSFGGKAGAADALVNQPVIFIHGNSDSALGQSSTLTGWNSSINYFLSQGYKT
                     SELYATTWGDANPLLSGNQYHSKPNLQKIRAFIQAVKAYTGAAKVDVVTHSMGVTLAR
                     KAILGGAASDAANGGSYNLGSPLTSGIDAFVGIAGANYGLVSCYQTGGSTPTCAATNG
                     LYPGYAAGSPPLGLSNILYNINYSSGSHYEGGYVYTIWSTADEVIGYGDVVYGRYTSR
                     IPGQNGEVVFGAYPYGHINSKDLTGYYQWRMVKYHAAN"
     gene            complement(1579..>2581)
                     /locus_tag="VGB68_11765"
     CDS             complement(1579..>2581)
                     /locus_tag="VGB68_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049959.1"
                     /translation="GLQWEFWNNFVFDVGYVGNKGTHLLQLLSPNQPVYNAATNSFAT
                     PFPGTAISALKNATGGVQQVSTTSLSNYNSLQMSLSKRFASGLQFLAAYTFGKSIDYY
                     SGGTVNELANVAGDQFNWRTNRGRSDFNREHRLVVSGVYAFPKRNYESGFARALFNDW
                     QIAGIAVFQSGLPFSIIDNPGNGVIQRANFNRNFNGDIYTTGGVSERLNGYFNTAAFV
                     RSCPIGTVVAGAFLACANNGVGAVNNPTFDPSNPFGNTLRNGFTGPGQKNVDISLIRI
                     IPFSEQFRGEFRLEAFNVFNWVNYANPNNNISGANFGRIERASTGPRVIQLGFKLNF"
BASE COUNT          634 a          689 c          681 g          577 t
ORIGIN      
        1 cataaatcgc cggacgcgct cctgtttatt caaatcgtcg gcggcgacaa acgccgctaa
       61 aacttccaaa tccggcgcga cgtgattttt tccgccgaaa ctcgtgcaag ccagaatcag
      121 cttttccagg cgttcgggaa aaccgagcgc gaattcctgc gcgacaaatc cgccgaatga
      181 aacgcccaga acgttcgcct tttcgatttg cagaaaatcg agcaaacgag cgacgtcggc
      241 ggcaatcgtc ggcagcgaaa tcgcttccgt taatttttcg tccgtcgccg agcgcgaaac
      301 gccgcacggg tcaaacgtta tgacccgaaa cgttttcgac aattcctcga tttgctcaaa
      361 ccacgcccac gcgcccgacg caaagcccgg aatcaaaatc agaggctcgc ccgcgccgtg
      421 tgattcgtaa taaagtttca cagagttgac tgaaagttga ctgaaagttg actgaaagtt
      481 tgctgaggtt aattgagttt ttcaactccc aaactcggtc aactttcagt caactcagtc
      541 aactaattag cggcgtgata tttcaccatt cgccattgat aatatccggt caaatctttc
      601 gaattgatgt gcccgtacgg atacgcgccg aagacgactt cgccgttttg accgggaatc
      661 cgcgaagtgt agcgaccgta aaccacatcg ccgtaaccga tgacctcgtc ggcggttgac
      721 cagattgtat aaacatagcc gccttcgtaa tgcgagccgg aagagtaatt gatgttatac
      781 aaaatatttg acaaaccgag cggcggcgag cctgcggcgt aaccgggata aagcccgttg
      841 gtcgccgcgc aggtcggcgt cgagccgccg gtttgatagc aggaaaccaa tccgtaattg
      901 gcgcccgcga tgccgacaaa agcgtcgatg cctgaggtca gcggcgagcc gagattataa
      961 cttccgccgt ttgccgcgtc gctcgccgcg ccgcccaaaa tcgctttgcg cgccagagtg
     1021 acgcccatcg agtgcgtcac gacgtcgact ttcgccgcgc ccgtataagc tttgacggct
     1081 tgaataaaag cgcgaatttt ttgcagattc ggcttcgaat gatattgatt gcccgacagc
     1141 agcggattcg catcgcccca cgtcgtcgcg tacagctcgg acgttttata accctgcgac
     1201 agaaaataat ttatcgagct gttccagccg gttaaagtcg agctttgacc gagcgccgaa
     1261 tcggaattcc cgtgaatgaa gattacgggt tgattgacga gcgcgtccgc cgcgccagcc
     1321 ttgccgccga agctgttgcc gccgtaatta aaattataac cgtcgtaacc gttggcgctg
     1381 agccagtttt gaaaatgcgt cgtcaattgc gccgaagccg cctgagccga aatcaaaact
     1441 aaaatcagga ccgaacacgc ctggataaaa ctttttttcc tgttcattgc tctttttcct
     1501 ttataaaaat tatggcggat gaaaaaggct ttcaactttt cacgactgaa agcctttttt
     1561 gttcgggctc gtttaacttt agaaattgag cttaaagccc agctgaatga cgcgcggtcc
     1621 ggtcgaagcg cgttcgatgc gcccgaaatt cgcgcccgaa atattattgt tcgggttggc
     1681 gtaatttacc cagttaaaga cgttaaaggc ttcgaggcga aattcgccgc gaaactgttc
     1741 gctgaacgga atgattctga taagcgaaat atccacgttc ttttgtccgg gtccggtaaa
     1801 accgttgcgc aaggtgttgc cgaacggatt cgacgggtcg aaagtcggat tgttgaccgc
     1861 gccgacgccg ttattggcgc aggcgagaaa agctccggcg acgaccgtcc cgatcggaca
     1921 cgagcgaacg aaagccgccg tgttgaaata accgttcaaa cgctcggaaa cgccgcccgt
     1981 cgtgtaaata tcgccgttga aattgcggtt gaaattggcg cgctgaatca cgccgttgcc
     2041 cgggttgtcg ataatcgaaa acggcaagcc ggattggaaa acggcgattc cggcgatttg
     2101 ccagtcgtta aacagcgctc gggcaaaacc cgattcatag ttgcgtttcg ggaaagcgta
     2161 gacgccgctg acgaccagac gatgttcgcg gttaaaatcc gagcgcccgc ggttcgtgcg
     2221 ccagttaaac tggtcgcccg ctacgtttgc cagctcgttg acggttccgc ccgaataata
     2281 gtcaatcgac tttccgaaag tgtaagcggc taaaaactgc aagccgctgg cgaagcgttt
     2341 cgagagcgac atttgcagcg agttgtaatt ggaaagcgaa gtcgtcgaaa cctgctggac
     2401 gccgcccgtc gcatttttca gcgccgagat agccgtgccg ggaaaaggcg tggcgaacga
     2461 gttggtcgcc gcgttgtaaa ccggctgatt cggactcaga agctgcaaaa gatgcgtgcc
     2521 cttgttgccg acatagccga cgtcgaaaac gaaattattc cagaattccc attgcaagcc
     2581 g
//
LOCUS       DASWNE010000600         1900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_56045, whole genome shotgun sequence.
ACCESSION   DASWNE010000600 DASWNE010000000
VERSION     DASWNE010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56045"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1283
                     /locus_tag="VGB68_11770"
     CDS             <1..1283
                     /locus_tag="VGB68_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049960.1"
                     /translation="RIYKILGTAAALHLFGLIVFSQVDLLGTKACDSPYVGKVCQVLD
                     AAYVSSVLLGTETEFSSRDYDKTEIDEEDVTWVNVSEQFNYPAGYFSQSNPAPELDPT
                     MMGIDPATGMTSSNIPGIPGGLMTSPSTLDAPPVLPTPNPNAVQGMPDSPFSIGNNST
                     ISSPKSPRPRTQKFPRVRTPKQKSPATLPDIPGLNENQTADKDKNPNANTTANKENKV
                     KTETPGGEESKLFNKKPLEDFGKKHSEAILKNDVDINAPFMIEVIAKLDDKGKLVKPR
                     MISTADSDPKMTLLAKEAILAFGDSQLLRTLYDVGARNVRIVFAQDKDNLQAIIKAET
                     ASENEAKTLQSGLNFALKNLYKPKEGSDEQILLDKAQLGVEGKNFVINFLIPNQEKSD
                     MIKKNLEKLKEKSNQSKPNGGGAETVSKDVNSAK"
     gene            1286..>1900
                     /locus_tag="VGB68_11775"
     CDS             1286..>1900
                     /locus_tag="VGB68_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049961.1"
                     /translation="MTRGKNYNSIVFLTTLSVYLGLVLVGATPQVLAQAALTRNFDIQ
                     IEAEVKDDLDNKPENDDLSKDVEAVRNFNIAQAIISFADDLKKLKSIGKVNYETDLNF
                     SQSLRTKLTFNGGYSVSKISSISNPWLETALEELLSNAEARDLISISDFLSDCEEKNC
                     RETSIEIHSNNDEFALTFGFTKSNAEKAGLAAEKFAGVFVSKKAA"
BASE COUNT          563 a          457 c          469 g          411 t
ORIGIN      
        1 caaggattta taaaattctc gggacggctg ccgctttgca tttattcggc ttaatcgttt
       61 ttagtcaggt tgatttgctg ggcaccaagg cttgcgacag cccgtatgtc ggcaaagtct
      121 gtcaggtgct tgacgccgct tatgtcagca gcgttctgct cggaacggaa accgaatttt
      181 ccagccgcga ttacgacaaa acggaaatcg acgaagaaga cgtgacgtgg gtcaacgtca
      241 gcgagcagtt taactatccg gcgggttatt tcagccagtc gaatcccgcg ccggagcttg
      301 acccgacgat gatgggaatc gaccccgcaa ccggaatgac atcgtccaat attcccggca
      361 ttccgggcgg acttatgacg tcgccgtcga cgctcgacgc gccgccggtt ttgccgacgc
      421 cgaatccgaa cgccgttcag ggaatgcctg attcgccttt ttcaatcggc aataactcga
      481 ccataagcag cccgaaatct ccgcgcccca gaacgcagaa atttccgcgc gtcagaacgc
      541 cgaaacaaaa atcgccggct acgcttcccg atattcccgg attgaacgag aatcaaacgg
      601 cggataaaga taaaaatccg aacgccaata cgacggcgaa taaagaaaat aaagtgaaga
      661 ccgagacgcc gggcggcgaa gaatcgaagc tttttaacaa aaagccgctg gaagacttcg
      721 gcaaaaaaca cagcgaagcg attttgaaaa acgatgtcga catcaacgcg ccgtttatga
      781 ttgaagtcat cgcgaaactc gacgataaag gaaaactggt taagccgcga atgatttcga
      841 ccgccgacag cgacccgaag atgactctgc tggctaagga agctatcctg gctttcggcg
      901 attcgcagct gcttcgaacg ctgtatgatg tcggcgcgcg gaatgtgcgc atcgtttttg
      961 cgcaggacaa agacaatcta caggcgatta tcaaagccga aaccgcctcg gaaaatgaag
     1021 ccaagacttt gcagagcggt ttaaatttcg ctttgaaaaa tttatataag ccgaaagaag
     1081 gttcggacga gcaaatttta ctggataagg cgcagctcgg cgtcgagggc aagaatttcg
     1141 tcattaattt cctgattcca aatcaggaaa aatccgatat gattaaaaag aatctggaaa
     1201 agcttaagga aaaatcgaac cagagcaaac cgaacggcgg cggagcggaa accgtgagca
     1261 aggatgtaaa ctcggctaaa taagcgtgac aagaggaaaa aattacaatt cgatagtttt
     1321 tctgacgacg ctctcggtct atctcggctt ggtgctggtc ggcgcgacgc cgcaggttct
     1381 ggcgcaggcg gctttgacgc gcaatttcga tattcaaatc gaagccgaag tcaaagacga
     1441 tttagacaac aagcccgaaa acgacgactt gagcaaagac gtcgaggcgg tcagaaattt
     1501 taatatcgcg caggcaataa tcagtttcgc ggacgattta aaaaagctca aaagtatcgg
     1561 caaggtaaat tacgaaaccg atttaaattt ttctcagagc ctgcggacta aattaacctt
     1621 taacggcggc tattccgttt caaaaatcag cagcatttca aatccctggc ttgagacagc
     1681 cctcgaagag cttctttcga acgccgaagc gcgggattta atttcaattt ccgattttct
     1741 ttccgattgc gaagaaaaaa actgtcgtga gacatcaatc gagattcatt cgaacaatga
     1801 tgaattcgct ctgactttcg gcttcaccaa atcaaacgcc gaaaaagccg gattagcagc
     1861 cgaaaagttc gccggcgttt tcgtatctaa aaaagccgct
//
LOCUS       DASWNE010000601         4394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_20300, whole genome shotgun sequence.
ACCESSION   DASWNE010000601 DASWNE010000000
VERSION     DASWNE010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20300"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..1222
                     /locus_tag="VGB68_11780"
     CDS             41..1222
                     /locus_tag="VGB68_11780"
                     /inference="COORDINATES: protein motif:HMM:NF015019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEY0049962.1"
                     /translation="MTLRGRSFTNTGVFLAILVGSIVGALMSVVTEYYTAMGKRPVNS
                     IIRQSETGAATNIIGGLSVGMESTLVPTIILAAGIYLSHYFAGLYGVAIAAAGMMATT
                     AMQLAIDAFGPIADNAGGIAEMSGLPKEVRARTDILDAVGNTTAATGKGFAIASAALT
                     ALALFAAFVGLAGISSIDIYKADVLAGLLVGGMIPFIFSSLCIAAVGRAAMAMVKEVR
                     RQFKEIPGIMEYKARPEYEKCVAISTKASLREMMLPGAIALTTPLIVGFIFGPEVLGG
                     LLAGVTVSGVLMGMFQNNAGGAWDNAKKSFEAGVMINGKMQYKGSEAHKASVTGDTVG
                     DPFKDTSGPSMNILIKLMSIVALIIAPHIALNSNQRAHPETAPKVQVERTVETHTEKK
                     L"
     gene            1382..1768
                     /locus_tag="VGB68_11785"
     CDS             1382..1768
                     /locus_tag="VGB68_11785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEY0049963.1"
                     /translation="MKTLKVQSKKREEMIEITREVESLLPAGGEGVLVLFTQHTTCGL
                     TINENADPDVKHDMLYWLRKEIPQYYADFRHFEHNSDAHIKSSLVGSSVTVPFEDGKL
                     LLGRWQGIYLCEFDGARERKVLVKII"
     gene            complement(1909..3081)
                     /locus_tag="VGB68_11790"
     CDS             complement(1909..3081)
                     /locus_tag="VGB68_11790"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HEY0049964.1"
                     /translation="MKKLSAFIPVHLWTIFLFVSVSCAQEFKIIQDGVEYAEMTRGAK
                     EEPVHINLLRLDLTKVRLDVARAMDAAIGLEKTSSIAARHGAIAAINAGFFRLDNSIF
                     RGDAAGILKVDGKILSESYNNRIALFISNKPSRTEVDFFHLTTRHELLYKNKSCGSVV
                     TGINRQRIENDFVLYTPEFHRTTLTNRDGLEIVFRNRKVTQIIDGKGSAVIPPDGFVL
                     SASGKYREQVKSCKIGTKIRELRFTNYDADDSSTRLSGARFPEIKAFTDSEDIVGGVP
                     QLIKNGKIEITWEQEKSSKAFVETRHPRTAVAKLKDGKFLMVTIDGRQAGYSVGMNLN
                     ELAAFLLELGALDAMNLDGGGSTTMFLEGKVVNKPSDKEGERSVSDAILVFPRKKM"
     gene            complement(3177..3659)
                     /locus_tag="VGB68_11795"
     CDS             complement(3177..3659)
                     /locus_tag="VGB68_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049965.1"
                     /translation="MRKFTNPLDNIKIAAPCAANWDEMYGNERRRFCSACKLNVYNLS
                     EMTREEAETFLINSEGRVCLRLYRRADGSVITQNCPLGWQALKRKASLAASAITALAI
                     AVFSGILGFRLFEAFSPKTPPEIYQPDYESAPKISFGGMTSNLSEIKLEILRSRRNRD
                     "
     gene            3822..4376
                     /locus_tag="VGB68_11800"
     CDS             3822..4376
                     /locus_tag="VGB68_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049966.1"
                     /translation="MINHISIGVHNPEKVARVLAELWDGTVIPFPPCPDSFIALANDG
                     KGTGVEITPINTVLVPGEGLPPEENFDSATPTEEYEAKFVADDYAPRYVATHLNVNTH
                     LSEREVKAIAEREGWRVLTCNRGGGLFQLIELWIEDRFMLEVMTPEQTARYVEIFSPE
                     SIAALQAQFLSAVPEPTTDLSLVG"
BASE COUNT         1079 a         1066 c         1105 g         1144 t
ORIGIN      
        1 tgcctgttac tttatcgtga tgtggatgct tcctgaaaat atgacgctca gaggtcgcag
       61 cttcacaaat acaggagttt ttctggcaat cctggtaggc tcaatcgtcg gcgctctgat
      121 gagcgttgtg accgaatact acacggcgat gggcaagcgt cccgtcaact caatcattcg
      181 acaatccgag acgggcgctg ccaccaatat tatcggcggt ctttccgtcg gaatggaatc
      241 gactctggtg ccgacaatta ttctggcagc cggtatttat ttatctcact actttgccgg
      301 tttatacggg gtcgctatcg ccgccgccgg gatgatggcg acaacggcta tgcagcttgc
      361 catcgatgct ttcggaccga ttgccgataa cgccggcggt atcgccgaga tgagcggtct
      421 gccgaaagaa gtcagagcga gaacggatat tttagatgcg gtcggaaata cgacggcggc
      481 aacgggtaaa ggttttgcga ttgcttcagc cgctttgacg gctctggctc tttttgccgc
      541 ctttgtcggt cttgccggta tcagctcgat tgatatttat aaagcggacg ttctggcggg
      601 attattggtc ggcggaatga ttccttttat cttttcttcg ctttgtattg cagccgtcgg
      661 cagagcggca atggcgatgg tcaaagaagt tagacgtcag tttaaagaaa ttccgggaat
      721 tatggagtat aaagcgagac cggaatatga gaaatgcgta gctatttcta ctaaagcatc
      781 tctgagagag atgatgcttc cgggcgcgat cgccttaact accccgctta tcgtcggatt
      841 tatttttgga ccggaagttc tcggaggttt attagcgggc gttaccgtct cgggcgtact
      901 gatgggaatg ttccaaaaca atgccggcgg agcctgggat aatgctaaaa aatcatttga
      961 agccggcgta atgattaacg ggaaaatgca gtataaaggc agcgaagctc ataaagcgag
     1021 tgtgacgggc gatactgtag gcgatccgtt caaagatact tcaggacctt ccatgaatat
     1081 cttaattaag cttatgagta tcgttgcatt aattatcgct ccgcatatcg cgcttaattc
     1141 aaatcagaga gctcatcctg aaacggcgcc gaaagtgcaa gtggaaagaa cggttgaaac
     1201 tcataccgaa aagaaactgt aaaagaaatc gtgtaacggt ttattaaaga acgctcttcg
     1261 ggcagaagcg gaaaccggag cttgtgcctg aatgagcaga ggtgaaaata ataatttagc
     1321 cgcggttatc cgcggctagt tttctttgtg ttatgcgtgt ccctgtggtg aaatttcttc
     1381 tatgaaaact ctgaaagttc aatcgaaaaa gcgcgaggaa atgattgaaa tcacgcggga
     1441 agtcgaaagt cttctgcccg ccggcggcga aggcgtcctt gttcttttca cgcagcacac
     1501 gacctgcggt ttgacgataa acgaaaacgc cgacccggac gtaaaacacg atatgcttta
     1561 ctggctgaga aaagagattc cgcagtatta cgccgatttt cgccattttg aacataattc
     1621 cgacgcgcac atcaagtcgt cgctcgtcgg atcgagcgtc accgttccat tcgaagacgg
     1681 aaaacttctg ctcggtcgct ggcagggcat ttatttgtgc gaatttgacg gcgcgcggga
     1741 gagaaaagtt ctggtgaaga ttatttagcc acagaggaca cagagaaaga aagaatgaaa
     1801 caacggatta atagggattt gcatagacta tttttgtaaa aattcgtgta atttaatgtt
     1861 aatccgttgt ttcaaatctt ctctctgtgt tctccgtgtc ctctgtggct acatcttctt
     1921 tcgcgggaaa accagaatcg cgtcgctgac gcttctttcg ccttctttgt ccgaaggctt
     1981 gttgacgact tttccttcca gaaacatcgt cgtcgagccg ccgccgtcca gattcatcgc
     2041 gtcgagcgcg ccgagttcca gcagaaatgc ggctaattca ttcagattca tcccgacgct
     2101 gtagcccgcc tgccgtccgt caatcgtgac catcaaaaat tttccgtctt ttaatttagc
     2161 gaccgctgtt cgcgggtgac gcgtttcgac aaaggctttt gaagattttt cctgttccca
     2221 ggtgatttcg attttaccgt ttttgataag ctgcggaacg ccgccgacaa tatcttcgga
     2281 atccgtaaaa gcctttattt ccggaaagcg agcgccgctc aatcgcgtgc tgctatcgtc
     2341 tgcatcgtaa ttggtaaacc gtaattcccg tattttcgtt ccgattttgc agctttttac
     2401 ctgctcgcga tattttccgc ttgccgataa aacaaaaccg tccggcggaa ttacggcgct
     2461 tccctttccg tcgatgattt gcgtaacctt ccggttccga aaaacaatct ccaaaccatc
     2521 tcgattagtc agagtcgtgc ggtgaaattc aggcgtgtag agcacaaaat cattttcaat
     2581 gcgctggcgg tttattccgg tcacgaccga gccgcagctt ttatttttat acaacagctc
     2641 gtgccgcgtc gttaaatgaa aaaaatcaac ttccgttcgg ctcggtttgt tgctgataaa
     2701 aagcgcgatt cgattgttgt agctttcgct taaaattttt ccgtccactt tcaggattcc
     2761 cgccgcgtcg cctctgaaga tgctgttgtc gagccggaaa aatcccgcgt ttatcgcggc
     2821 aatcgcgccg tgccgagcgg caatcgaaga agttttttcc agcccgattg ccgcgtccat
     2881 cgcgcgggcg acgtcgagcc ggactttcgt taaatcgagt ctcagcagat tgatatgaac
     2941 cggttcttcc tttgcgccgc gcgtcatttc ggcgtattcg acgccgtcct gaataatctt
     3001 gaattcctgg gcgcaggaaa cggagacgaa aagaaaaatc gtccacagat gaaccgggat
     3061 aaaggcggat agtttcttca taaatttaaa acagcgccgg cgaaacggaa ctttacaaaa
     3121 aatataaagt ctttccgccg aaaaatgaag cgaatccgcg aatcaatccg cgccgctcaa
     3181 tcccggttgc ggcgacttct taaaatttcg agtttaattt ccgacagatt gctcgtcatt
     3241 ccgccaaaac ttattttcgg agccgattca taatccggtt gataaatttc cggcggcgtt
     3301 ttcgggctga aagcttcaaa caatcgaaac cccagaattc cgctgaaaac agctatcgcc
     3361 agcgccgtta tcgccgaagc cgccagcgaa gcttttcttt tcagcgcctg ccagccgagc
     3421 gggcaattct gcgtgataac gctgccgtcc gcgcggcgat aaaggcgcag gcaaacgcga
     3481 ccttccgaat ttatcagaaa agtttcggct tcttcgcgcg tcatctccga aaggttatag
     3541 acgttcaatt tgcaggctga acaaaatctc cggcgctcgt tgccgtacat ttcgtcccag
     3601 tttgccgcgc acggcgcggc gattttgatg ttgtccaatg gatttgtaaa tttccgcata
     3661 tcgacctccg cctgattaga caaccggaaa cgaattttgt tccgcccgga aggaaataaa
     3721 ttcgtcggct ctggtacttt ttgctttgaa atttcgctat agcaattgag gagcaaaatg
     3781 ctccggcaat tatttattta ttcaaaggag caatgtttca aatgataaat catatttcca
     3841 tcggagttca caacccggaa aaagtcgcgc gagttttagc cgaattgtgg gacggaacgg
     3901 ttattccttt cccgccgtgc ccggattcgt ttatcgcgct cgccaatgac ggcaaaggca
     3961 cgggcgtcga aatcacgccg attaataccg ttttggtgcc gggcgaaggc ttgccgccgg
     4021 aagaaaattt cgattcggcg acgccgaccg aagaatacga agcgaaattc gtcgccgacg
     4081 attacgcgcc gcgctacgtc gccacgcatc tgaacgtcaa cacgcatttg agcgagcgcg
     4141 aagtcaaggc aatcgccgag cgcgaaggct ggcgcgtgct gacctgcaat cgcggcggag
     4201 gcttgtttca attaatcgag ctgtggattg aagaccgctt tatgctggaa gtgatgacgc
     4261 cggaacagac ggcgcgttat gtcgaaattt tcagccccga atccatcgcc gctctgcagg
     4321 cgcagtttct gtcggctgtg ccggagccaa ccacggattt gagtctggta ggttagcgta
     4381 aaaataaaaa tgaa
//
LOCUS       DASWNE010000602         3800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_56274, whole genome shotgun sequence.
ACCESSION   DASWNE010000602 DASWNE010000000
VERSION     DASWNE010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56274"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="VGB68_11805"
     CDS             <1..754
                     /locus_tag="VGB68_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049967.1"
                     /translation="GSFVTHQVKVKGLTTLLYENGNSGIGVRRNASLYPDDKVLSNRE
                     FIYQTPIVALTNPLTPFVDNPTPVAIYPTVVNQTNLDLGDYMRLTLEAMMGLTEVNAE
                     LLKQTEETSALANRRLKIDARYGFPIGSPSGSSVETDYFLPLYPAILVRSFDLPVDGL
                     QNALKEWVGQSGDSGTNGIKPYAAVLADWLQNSALPLGQPTSPDAPPKGAILVLDITL
                     YSQLNSGVNQLPLLRLSDLRLDLSVVAAPKAT"
     gene            complement(1118..1498)
                     /locus_tag="VGB68_11810"
     CDS             complement(1118..1498)
                     /locus_tag="VGB68_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049968.1"
                     /translation="MKWTVGNIERERFIEVILEGEFSIVEFSDIFQEILSRESGRQGI
                     HILFDDTGLIFKDTNLDMIREASNCYAKDHYRIGDGRIALVMSSLADFIRGRQFEILA
                     GIKIPSNIGVFRDKEEALNWLLND"
     gene            1737..1985
                     /locus_tag="VGB68_11815"
     CDS             1737..1985
                     /locus_tag="VGB68_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY0049969.1"
                     /translation="MLISAVYTDFSKLSIAGARIALAGDYLSYPRYATTNPFGYYRFV
                     NVPIYFRYQAAVFRQKPSLSAARCFHLSRRPSKFRLYE"
     gene            complement(2070..3080)
                     /locus_tag="VGB68_11820"
     CDS             complement(2070..3080)
                     /locus_tag="VGB68_11820"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEY0049970.1"
                     /translation="MKVYTTLGDGIDRLKLNELPLPKIKPNEVLVKMTAVSLNYRDLL
                     VIKGIESWKPAAPRIPISDGVGEIVALGEGVSRFRAGDRVAGIFLPKWLDGELTKEKY
                     VLPLGGAAANGVLAEYVSFDEQAVVKIPDNLSGEEAAALPVAALTAWHAVARRSNVQP
                     GETVLIQGTGGVALFATQFVHASGGLPLVISSSDEKLEKAKKLGASSIVNYKRFPDWE
                     EQILELTNGKGVDHVIETVGGENLNRSLNAVKLSGTISFIGLIAGLSAPINTYQFVTK
                     NVQLYGIETGSREMFEQMNRFIEENQLKPVIDKTFALSEIREALKYLESGRHFGKIAI
                     NF"
     gene            complement(3228..>3800)
                     /locus_tag="VGB68_11825"
     CDS             complement(3228..>3800)
                     /locus_tag="VGB68_11825"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEY0049971.1"
                     /translation="YKDLLTGWETADSITIDAHKWLNVPYDAAMIFTRHRSLQIEVFQ
                     NSGAYLGAMAEPPDFFHLSPENSRRMRALPAWFTLLAYGREGYREIVERNCETARLLG
                     KKIENSTKFRLLAPVRMNVVCFTLNSDNPSAEIIREFLSQLRSDGRVFLTPTVYNGTP
                     GVRAAFSNWRTTEKDIEIAWQAMNDCAPET"
BASE COUNT         1012 a          960 c          801 g         1027 t
ORIGIN      
        1 cggcagtttc gtaacccatc aggtcaaggt caaagggctg actacattgc tctacgaaaa
       61 cggcaactcc ggtatcggag tcagacgaaa cgcgtcgctt tacccggacg ataaggtgct
      121 atcgaatcgc gaattcatct atcaaacccc gatagtggcg cttaccaacc cgctgacgcc
      181 atttgttgat aatccgacgc cagtcgcaat ctacccgact gttgtaaacc agacaaatct
      241 ggatttgggc gactatatgc gattaaccct cgaagcgatg atggggctga ctgaagtcaa
      301 tgccgagttg ctgaaacaaa ctgaagaaac ttccgctctg gctaaccgcc gtctcaaaat
      361 cgacgcgcgt tacggcttcc cgattggctc gccgtccggc agcagcgtcg agacggacta
      421 cttcctgccg ctttaccctg caatcctggt gcgctcgttc gacctgcccg ttgacggtct
      481 gcaaaacgcg ttaaaagaat gggtcggaca atccggagac tccggtacta atggaatcaa
      541 accatacgcc gcagtattgg ctgattggtt gcaaaattcc gctctgccgc tcggacagcc
      601 aacgtctccc gatgcgccgc ctaaaggtgc aatattggtg ttggacatta cgttgtattc
      661 acaactgaat tcgggcgtaa atcaacttcc actgttgcgt ctttccgatt tgcgccttga
      721 cctttcagtc gtcgccgcgc ctaaagcaac ttgagaagga accaaagccg ggcaaaataa
      781 aggcgtgtaa aaacttaaaa ccgggttcgc tcgcgaataa atgcgttgtc attcatctga
      841 caacgcattt attcgcgtgc gggaaacgca gtgaatccgg ctttctgctc agcggcggaa
      901 aagctctctc gtatcagaga atatattggg taagaaattt tcattatatg agctaaaaat
      961 agcagaaatt ttcactttat gcgctacact tttttcactt tatgcgcaat tttattttcg
     1021 aagttgttga ttttactaga agcccatttt cactttatga gcgatctaac aaagatgaat
     1081 aaagcggcaa agttccggat ttcaggtatc gaagaattta atcgttcaat agccaattga
     1141 gagcttcttc tttatccctg aaaactccga tatttgaagg tatcttaatt cctgccagta
     1201 tctcgaactg ccgtcccctt ataaaatctg caagcgaact cattacaagc gctattctgc
     1261 cgtctcctat acgataatga tctttagcat aacaattgct tgcttccctt atcatatcca
     1321 gatttgtatc cttaaagatt agtccggtat catcaaataa aatatgtatt ccctgacgcc
     1381 cggattcacg cgacagtatt tcctgaaata tgtcggagaa ctcgactatt gaaaattctc
     1441 cttcaagaat aacttcaata aaacgttccc gctcaatatt acctactgtc catttcatag
     1501 ttcataatca tggacagatt ttaatattta tttaagacaa taacgatatt ttattatggc
     1561 gatcatatac taatcatatc caaaaacaga attatattaa gcaatcagca atgaaacaac
     1621 cgaaacttaa accgtgaagt cgcattgctg aaaaatcttt gctttataag tttaatgagc
     1681 gcataagtcg ataacttcgg gtgcagtcgg cataaataat cctccattaa aaataaatgt
     1741 tgatttctgc cgtttacaca gatttttcta aactctccat cgcgggcgct cgcattgctc
     1801 tggcaggcga ttatctttcc tacccgcgtt atgcaacgac caatcctttc ggctattaca
     1861 ggtttgtcaa cgtcccgatt tattttcgct atcaggcggc ggttttccgc caaaaaccat
     1921 cgctttccgc cgcccgctgc tttcatctta gtcggagacc gagcaaattt agactttacg
     1981 aataatcaat aaagataatc tcgaaaaatc attggatatc gggaaagctc taaagtggtc
     2041 ttgccaaaca cgaaaatgcc aaagatttgt taaaagttga ttgcgatttt cccaaaatgg
     2101 cgaccgcttt ccagatattt cagagcctct cggatttcgc tcaaagcgaa agttttatca
     2161 atcaccggtt ttaattgatt ctcttcgata aagcggttca tctgctcaaa catctccctc
     2221 gagccggttt caattccata tagctgcacg tttttagtca cgaattggta ggtattaatc
     2281 ggcgcgctca aaccggcaat caatccgata aaactgattg tgccgcttaa tttgacagca
     2341 tttaacgacc gattgagatt ttcgccgcca accgtttcaa tcacgtggtc gactcctttg
     2401 ccgtttgtca attcaagaat ttgttcttcc caatcaggaa accgcttata attaacgatt
     2461 gacgaagcac cgagtttttt agctttttcg agcttctcgt cgctgctcga aatcaccagc
     2521 ggaagtccgc cgcttgcgtg aacaaactgc gtcgcaaaaa gcgcgacgcc gcccgttccc
     2581 tgaattaaaa cagtttctcc gggctgaaca ttgctgcggc gagcgaccgc gtgccaggct
     2641 gtcaaagccg ctaccggcag cgccgccgct tcctcgccgg acaaattatc cggaattttg
     2701 acgacggctt gttcgtcaaa agaaacgtat tccgccaaaa ctccgtttgc cgcagcgccg
     2761 ccgagcggca aaacgtattt ttcttttgtc agctcgccgt cgagccattt cgggagaaaa
     2821 atgcccgcta cacggtcacc ggctctaaac cgggaaacgc cttcgccgag agcgacgatt
     2881 tcgccgactc catcggaaat cggaatgcgc ggcgcagcag gtttccagct ttcaatgccc
     2941 ttgataacca gcaaatctcg ataattcaac gaaactgcgg tcatttttac caaaacttca
     3001 ttaggtttga ttttcggcaa aggaagctcg tttaatttca gccggtcaat gccgtcgccg
     3061 agtgtcgtgt aaactttcat attcagttaa tcgtaaaaat cttccttcac ttttttactc
     3121 gcgggaagct ttgcaaatgc atcagaaaac agttaaaagc cgcccgatat tattccgccc
     3181 gaaagccgga aagatgccgt ttggaagcta tcaaataaag cattaaatta tgtttcgggc
     3241 gcgcagtcat tcatcgcctg ccaggcgatt tcaatgtcct tttcagttgt tctccagttg
     3301 ctgaaagcgg cgcggacgcc cggcgtcccg ttataaacag tcggcgtcag aaacacgcgt
     3361 ccgtcgctgc gcaattgtga taaaaactct ctgataattt cagcgctcgg attgtcggaa
     3421 ttgagcgtga agcaaaccac gttcattcgc accggggcga gcaatcgaaa ttttgtagag
     3481 ttttctattt ttttccccaa caggcgcgcc gtttcgcagt tgcgctcgac aatttcgcgg
     3541 taaccttcgc gcccgtatgc cagcaaagtg aaccacgctg gtaatgcgcg catccggcgt
     3601 gagttttccg gcgacaagtg aaaaaaatcc ggcggctccg ccattgcgcc gagatatgca
     3661 ccgctgtttt gaaaaacctc gatttgaagg ctgcgatgac gagtaaaaat catcgccgcg
     3721 tcatacggaa cgttaagcca tttatgagcg tcaatcgtaa tactgtcagc cgtctcccag
     3781 cctgtcagca aatccttgta
//
LOCUS       DASWNE010000603         2661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_90327, whole genome shotgun sequence.
ACCESSION   DASWNE010000603 DASWNE010000000
VERSION     DASWNE010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90327"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2658)
                     /locus_tag="VGB68_11830"
     CDS             complement(<1..2658)
                     /locus_tag="VGB68_11830"
                     /inference="COORDINATES: protein motif:HMM:NF039753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bpX6 domain-containing protein"
                     /protein_id="HEY0049972.1"
                     /translation="MLSEAEMRRRILTFRQEGMQVFRRGNDLIVRLPKALRVDSTGSL
                     GLPFVRYGEILSAFPIKKKDLERLGDLGDGLIFLSEGNLETVHRQDLKPEKIESWFEV
                     ADFRVIETETLGETVPEAVRVMKAISEDDVNLRADLKDVPPADSQLAEILAKLKEVKA
                     ANENKKSGSFFSSSDTSDSSESSLTNFFSTVFGNLKNLFASTGTTFGANSGYTKTAAT
                     DASPPEQFEPSAFSKIYNRLRHLTVKAVVEMRMAKIFGRRQAKYLAEMMEKFESGDLS
                     EALKYAVPLEDMQALRDFMEKPVSLFVPRPRSNLQISFGQNAGGSTINLEGEWFEHLR
                     QLYRTSFERLEAQGRLEEAAFVLAELLKSNAEAVEFLSKHGKFRLAAELAEARNLPKE
                     TVVRQWFLAGEKMRAVRLAILHNCFEYAVTKLEQENSGMGAELREVWAESLAESGNFP
                     AAVDVIWKLEQRRERAKEWMEKTIEFGGAPAARMLARKIMLFPENFNEIKEKFQEIIQ
                     DDEPEAIEKRNAFARSVFKQSINDELRTLLRPLARKILSDALKTSQSLELKEFRELVM
                     MAKDNALRTDLPTFPQLLSPAASGGCFEITISADDKGASFIYDACLLPDGKIALALGE
                     VGVKILSRYAKTIAYFDQPAQKLVISDFSNKAIALAKRGETLRLARIDFIERRAEFWC
                     DAKLDVFTPNFDGNTWFVGLKDEFYAIDANAENFEAVWRVPDVGGTVYSVARSAGQAK
                     FLTLNTKGFETWWYELPNLTLRSRNERKWLDNIGESVLYNANISEGGHSVVIIQEQIA
                     DSKDLRFYANIFDHEHLTVKFEFPLDIKGFNRPDIFSQYSVVTAYSEQYAKVYVFFSS
                     GNLIAVFTLELARNVSTKFNGNFLIITDDC"
BASE COUNT          655 a          692 c          601 g          713 t
ORIGIN      
        1 gcaatcgtcg gtaataatca aaaaatttcc gttaaattta gtcgagacat tccgggctag
       61 ttccagagta aaaactgcta ttaaatttcc gctgctgaaa aatacataga cctttgcgta
      121 ttgttcggaa taggcggtta cgactgaata ctggctaaaa atatcgggtc ggttaaatcc
      181 ttttatatcg agtggaaact caaacttcac cgtcaggtgc tcgtggtcaa aaatgtttgc
      241 ataaaagcgc aaatcttttg aatcggcgat ttgctcctgt attattacga ccgaatgacc
      301 gccctcggaa atatttgcgt tatacagaac gctctcgcct atgttatcga gccatttccg
      361 ctcgttacgg cttcgtaaag ttaaattcgg caattcatac caccaggttt caaagccctt
      421 agtattgagc gtcagaaatt tcgcctgacc ggcagagcgg gctaccgaat aaactgtccc
      481 gccgacatcg ggcacgcgcc acacggcttc aaagttttcg gcatttgcgt caattgcata
      541 aaattcatcc tttaagccga caaaccaggt attaccgtcg aaattcggcg taaagacgtc
      601 caatttcgca tcacaccaga attcggcgcg gcgttcaata aaatcaattc gagcaagtcg
      661 taaggtttcg ccgcgtttgg ctaaagcaat tgctttattg gaaaaatccg aaatgacaag
      721 cttctgcgca ggctggtcaa aataggcgat tgttttcgcg taacgcgaaa gtatcttaac
      781 gccgacttcg ccgagtgcca gggcgatttt cccgtccggc agcaagcagg cgtcataaat
      841 gaagctcgct cccttgtcat ctgctgaaat cgtaatttca aaacatccgc cgcttgcagc
      901 cggagataaa agctggggga aggtcggtaa atcagtccgc agggcgttgt cttttgccat
      961 cattaccaac tcgcgaaatt ctttcaattc cagactctgc gaagttttca gcgcatcgct
     1021 taaaatcttt cgcgctagcg ggcgaagcag cgttcgcagt tcgtcattaa tgctttgttt
     1081 aaaaacggaa cgggcaaagg cattacgttt ttcgattgct tcgggctcat cgtcctggat
     1141 aatttcctga aacttttctt ttatctcgtt gaagttttcg gggaaaagca taatctttct
     1201 ggctaacata cgcgccgccg gcgcgccgcc gaattcgata gttttctcca tccattcctt
     1261 cgcccgttca cgtctctgct cgagtttcca aatgacatcg acggcggcgg gaaagttgcc
     1321 gctttcagct aaagattccg cccacacctc acgcaattca gctcccatgc cggaattttc
     1381 ctgttccagt ttcgtgacgg catactcaaa acaattgtgc aggatagcga gccggacagc
     1441 ccgcattttt tcgcctgcta aaaaccattg ccggacgacg gtctctttcg gcaaattgcg
     1501 agcttcggca agctcggctg ccagccggaa tttgccgtgt tttgaaagaa attcgacggc
     1561 ttcggcattg ctttttaaaa gctccgctaa aacaaaagcg gcttcttcga ggcgtccctg
     1621 cgcttcgagt cgctcgaaag acgtgcgata aagctgtcgc agatgctcga accattcgcc
     1681 ttcgagatta atagtcgagc cgcctgcgtt ttgaccaaag ctgatttgca gattgctcct
     1741 cgggcgcggc acgaaaagcg agacgggctt ttccataaaa tctcgaagcg cctgcatatc
     1801 ttccagcggt acggcgtatt taagagcctc cgataaatcg ccgctttcaa atttttccat
     1861 catttccgcc agatatttcg cttgccgccg cccgaaaatt ttcgccatcc gcatttcaac
     1921 gacagccttg accgtcaaat gccgcagccg gttgtagatt tttgaaaagg cgctcggctc
     1981 gaattgttca ggaggcgagg catcggttgc ggctgttttc gtataaccgg aatttgcgcc
     2041 gaaagtcgtt ccggttgacg cgaaaaggtt ttttaaattt ccgaaaaccg ttgaaaagaa
     2101 atttgtcagg gagctttccg aactgtcgga agtatcggag cttgagaaaa aagagccgga
     2161 tttcttattc tcattcgccg cttttacttc ctttaatttt gccagtattt ccgcgagctg
     2221 cgagtccgcc ggcggcacgt ctttaaggtc ggcgcggaga ttcacatcat cttcggaaat
     2281 cgccttcatc acgcgcaccg cttccggtac ggtttctccg agcgtttcgg tctcgattac
     2341 gcgaaaatcc gcaacctcaa accagctctc gattttttct ggctttaaat cctgccggtg
     2401 aacagtttcg agattcccct ccgaaagaaa aattaaaccg tctcccaaat cgccgagtct
     2461 ttccaaatcc tttttcttta tgggaaatgc cgaaagaatt tcgccgtagc gaacaaacgg
     2521 caatcctaaa cttcccgttg aatcgacgcg taacgctttc ggcaaacgca cgattaaatc
     2581 attcccgcgc cgaaaaacct gcatgccttc ctgccggaat gtcaggatac gccggcgcat
     2641 ttccgcttcg ctcagaattt t
//
LOCUS       DASWNE010000604         1657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_585305, whole genome shotgun sequence.
ACCESSION   DASWNE010000604 DASWNE010000000
VERSION     DASWNE010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585305"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            329..>1657
                     /locus_tag="VGB68_11835"
     CDS             329..>1657
                     /locus_tag="VGB68_11835"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEY0049973.1"
                     /translation="MLNKFRDKFLLETVVFVSGALVMIFEIIGSRLLAPYIGTSTYIW
                     TSLIGVILGSLSVGYWLGGRIADRKPDLKILSGILFFAGALTTLTLFLHEIILSILAT
                     ASIRLELKAIIAALVLFAPASVLFGFVTPYAVRLKMLSVEDSGKIVGRLYALSTVGSI
                     SGTFAAGFVLLPFVGSARSLYLIIAALFLLSMLLGAFQLTTKKLYVLILFPAAIGLNE
                     LYAYSLYRSHDLHDFDTHYSRLRVFRAIDERTQKPMTALTTDPLTTQSAIFLDGDELV
                     LRYAPYYHLLRHFKPDFQNTLIIGGAGYTFPSEYLRKYPDKKIDVVEIDPQMTEIARR
                     FFRLKDNPNMRIFHEDGRVFLNQTTDKYDVVLIDAFGSVYSVPFQLTTLEAVRKIRDV
                     LADDGVVILNMISAIEGEGSLFLQAEYKTFQAVFPNVYLFKVRAERDDKTP"
BASE COUNT          379 a          405 c          406 g          467 t
ORIGIN      
        1 gtgcgcgcag taaggacagg tcaaaagccc gttttgcgga ttgaaaacca tattcgcgcc
       61 gcagttttcg cataaaaaac ggttgatgtt ttcgcgtttg atttcaggtg cattttgcat
      121 agagttaaag taaagacttc ataatcgcag aatataaagg ggaaattcaa acaggataga
      181 caggatacac aggataaatt gtttttcaaa tgaacatttg agttttattc aaaagttttg
      241 aataaaaaag ctcccatcct gtttatcctg tccatcctgt taaaatttcc ctttgaattt
      301 tccggtagat tatggctcaa tcgaatcgat gctcaacaaa tttcgcgata aatttctgct
      361 ggaaacggtt gtctttgtgt cgggcgcgct ggtgatgatt ttcgaaatta tcggttcgcg
      421 gctgctcgcg ccatatatcg ggacttcgac atatatctgg acgagtttaa tcggcgtgat
      481 tctgggcagt ttgagcgtcg gctactggct cggcgggcgc atcgccgacc ggaagccgga
      541 tttaaagatt ttatcgggaa ttttattttt cgcgggcgct ttgacgacct tgacgctttt
      601 tctgcacgaa attattctct cgattctggc aaccgcttcg attcgactgg agctgaaagc
      661 catcatcgcc gcgctcgttt tatttgcgcc cgccagcgtt ttgttcggtt tcgtcacgcc
      721 gtatgcggtt cgcctgaaaa tgctgagcgt cgaagattcc ggtaaaatcg tcggcaggct
      781 ttacgcgctt tcgaccgtcg gcagcatctc gggaaccttc gccgccggat tcgtactgct
      841 gccgtttgtc gggagcgctc gctcgcttta tctgattatc gcggcgctgt ttctgctttc
      901 gatgctgctc ggcgcgtttc aattgacgac gaaaaagctc tacgttttga ttttgtttcc
      961 ggcggcaatc gggctcaacg agctttacgc ttattcgctg tatcgaagcc acgatttgca
     1021 cgatttcgac acgcattaca gccgcctgcg cgtctttcgc gcgattgacg aaagaacgca
     1081 gaaaccgatg acggcgctga cgaccgaccc gctgacgacg cagtcggcga tttttctcga
     1141 cggcgacgag ctcgttttgc gttatgcgcc gtattatcat cttttacgtc atttcaaacc
     1201 cgattttcaa aacactctca tcatcggcgg cgcgggctac actttcccgt ccgaatacct
     1261 gcgaaaatat cccgacaaaa aaatcgacgt cgtcgagatc gacccgcaaa tgaccgaaat
     1321 cgcgcgccgt ttttttcgcc tgaaagataa tccgaatatg cgcatctttc acgaagacgg
     1381 gcgcgttttt ctgaatcaaa caacggacaa atacgatgtc gtgctaatcg atgctttcgg
     1441 ctcggtttat tccgtgccgt ttcagctgac gaccctcgaa gccgtgcgaa aaatacgcga
     1501 tgttctggcg gacgacggcg ttgtcatttt gaatatgatt tcggcaatcg agggcgaagg
     1561 ctcgctgttt ctgcaagccg aatacaaaac ttttcaagcg gttttcccaa acgtttattt
     1621 gtttaaggtg cgcgccgaac gcgatgataa aacgccg
//
LOCUS       DASWNE010000605         4814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_159625, whole genome shotgun sequence.
ACCESSION   DASWNE010000605 DASWNE010000000
VERSION     DASWNE010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159625"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..256)
                     /locus_tag="VGB68_11840"
     CDS             complement(92..256)
                     /locus_tag="VGB68_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049974.1"
                     /translation="MNVTFRAELMPGKSREERTFRIAEVLPNNRVILHDFTGEHRESE
                     FEPINFERRK"
     gene            complement(345..503)
                     /locus_tag="VGB68_11845"
     CDS             complement(345..503)
                     /locus_tag="VGB68_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049975.1"
                     /translation="MKKKRLVERPVFDAIRKPTAPPGQKIGKDKPDEKARPARRQVKH
                     KKPENPDE"
     gene            669..1670
                     /locus_tag="VGB68_11850"
     CDS             669..1670
                     /locus_tag="VGB68_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049976.1"
                     /translation="MAVRPKNNKAVVSFAPTGKRSDYRTYRRPFWLPAPAYYILAAAI
                     SAAVFFLVWGILHEGEEMPWIPAGILASFSIATAVVVREFFLRRARNQFLRAQKRLDF
                     NLTSAAQHKSKDSNNKLTIEKNAAILRNIERKSEAARVLGKLPDAHWEVFEICNEYLQ
                     ITERELQTVGVGSPRLPALRLGREKIHELHRFHLLAWASVETRTLTQEARIRATIAEK
                     LETAQKALAVLDAALQFYPNEAELTESATAVEEFIASIKVSHWIEQAERSAFKGNYKR
                     AVSHYRDALFYLARENVRSEEREMIAEKINAEIEKLRDISAVEEDKKQISPESKKKK"
     gene            2025..3017
                     /locus_tag="VGB68_11855"
     CDS             2025..3017
                     /locus_tag="VGB68_11855"
                     /inference="COORDINATES: protein motif:HMM:NF022007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HEY0049977.1"
                     /translation="MLWLQGIAHAQTPAQTPPASETDNTKTVHFGDLIDVDVVGSAEY
                     DWRGTITPEGFLSGIDFVEEPIYALCRTEDDIARQVEKAYGKLLREPKVVVRVLDRSG
                     RPVSVLYGAVKTPQRFQIQRSVRLNELIILSGGITERASGEIQIFRPQYLSCQAEKKS
                     DENKTALDEEKRTSFVSTRREDNGSTYINIKIADLLAGKADANPLILTGDIVTILEAE
                     PIYIIGGVAAPKQISSRSQITVSRAIATAGGLTKEADGTKIVIFRRNGRENKTIEVDL
                     DAVKAEKAEDIVLQAFDVVDIPQKGKTKRKFPPVIRSIEGGEKNALNLPLRIVD"
     gene            3088..3309
                     /locus_tag="VGB68_11860"
     CDS             3088..3309
                     /locus_tag="VGB68_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049978.1"
                     /translation="MAEKTGKYSRRTIALFGLVAVGILIAVLVGLQQIAILYVLATIS
                     LVALLLTVAFADLEKVGRENSEGFTPRSE"
     gene            3368..4660
                     /locus_tag="VGB68_11865"
     CDS             3368..4660
                     /locus_tag="VGB68_11865"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEY0049979.1"
                     /translation="MIFSLSAPGLTPQISDEAKAVRLKWKTGTINIAFSSSLTKPPQN
                     IKYESDVVGAARRSLATWEKAADIKFNETFSDKQSVSPAGNAGDGVSLITVASTPENI
                     LLFGDGSEISAKTRVFYNRRGIITEADIVLNPYMQFSTDGTPGTFDLEATITHEIGHL
                     LGLEHSSLVGSTMQTHQGKNGIYNLPQFASRTLSDSDLANLRARYGAKADEDCCGAVK
                     GKITLAPTAAANNFHVWLEESETGKIQAGTITQADGTFYLDGLEAGKYRVLAQDSGEK
                     QKNRKKIFSGGEIGEVVIDKGKTAYIEKQLALKERSFSVKYIGFNGQISNIPLPVDGG
                     KSFVIYLGGENLNPNELIISTGTPFIKVEKNMTVAHDFGDEISVLSFEISVQPNVPPG
                     EYSLRLQKNNGEVEYFVGGIINDAIFNPASSPLISDFD"
BASE COUNT         1326 a         1111 c         1236 g         1141 t
ORIGIN      
        1 tcaggattca agattcagaa tttaagattc aggatttgcc gttagccggt aaccgatcct
       61 gaattttgaa ttttgaatcc tgaatcccga attatttccg gcgttcaaaa ttaatcggct
      121 cgaactcgct ctcgcggtgc tcgcccgtga aatcgtgcag aatcacgcga ttgttcggca
      181 gaacttcggc aattcgaaaa gttctttcct cgcggctttt acccggcatc agctcggcgc
      241 gaaacgtaac gttcatttcc ggtcgcgccc aaacgctggg ctttataatt ttgcggaaga
      301 aattttgaaa tatgctcatt cgtataatta aaggctggac ttttttattc gtccgggttt
      361 tccggctttt tatgtttcac ctggcggcgc gcgggacgcg ccttttcatc gggcttatct
      421 tttccgattt tttgaccggg cggcgcggtc ggtttgcgaa tcgcatcgaa aacgggtcgc
      481 tcgaccaatc tttttttctt catattgaaa aaaaattata acaaaaattg ttccggcagc
      541 gcttgatgtt gacataaaga aaagaaaaat ccactataaa aaagaactgt gttaggcttt
      601 aaaaacgatt atcttcacgc aaaaaataaa aaatacattt ctccaagttc aaattcggga
      661 gaaaattctt ggctgtcaga ccgaaaaaca acaaggctgt cgtaagtttt gctccaacag
      721 gtaaacggag cgattacaga acttaccggc gaccgttttg gcttcccgcg ccggcttatt
      781 atattttggc ggcggcgatt tcggctgccg tcttctttct cgtctggggc attttgcacg
      841 agggcgaaga aatgccctgg attccggcgg gaattctggc gagcttctcg attgcgacgg
      901 cggtcgtcgt gcgcgaattt tttctgcggc gtgcccgcaa ccagtttctg cgggcgcaaa
      961 aacggctcga ttttaattta accagcgccg cgcagcacaa aagcaaagat tcgaacaata
     1021 agctgacgat tgaaaaaaac gccgccattt tgcgaaatat cgagcggaaa tcggaagcgg
     1081 caagagttct cggaaagctg ccggatgcgc actgggaagt ttttgaaatc tgcaacgagt
     1141 atttgcagat aaccgaaaga gaactgcaga cggtcggcgt cggctcgccg cggcttccgg
     1201 cgctcaggct cggcagagaa aaaatacacg agctgcacag gtttcatttg ctcgcctggg
     1261 cgtcggtcga aaccaggacg ctgacgcagg aagcgcggat tcgtgctaca atagctgaaa
     1321 agctggaaac ggcgcagaag gctctggcgg tgctggacgc ggctctgcaa ttttatccga
     1381 atgaagccga attaaccgaa tcggcgacgg cggtcgaaga atttatcgcc tcgataaaag
     1441 tttcgcactg gatcgaacaa gccgagcgca gcgcctttaa aggcaattac aaacgcgccg
     1501 tcagtcatta tcgcgacgcg cttttttatc tcgccagaga aaacgtccgg agcgaggaaa
     1561 gagaaatgat tgccgaaaaa atcaatgcgg aaatcgaaaa attgcgcgac atttccgccg
     1621 tggaagagga taaaaaacaa atttcacctg aatcgaaaaa gaaaaaatga ttagtcaaag
     1681 atgtccaaaa tgcggaagta agcgagtccg tcgaggctat cgcccgacgc ctttctggtt
     1741 gaaaattttt ttccgctaca acctgctgtg cgataactgc aactgggaat ttaaaggctt
     1801 tgccgtcccc ggcacggttt cttcaaaacc gactcggaaa ccgctgaata agacgcttca
     1861 ataaacgcga aaaagctaaa cgggaagatg aattttcgaa aacttgcaaa agaagtttcc
     1921 gaaaacagtg gaaaaatgtg taaaatcggg taaacgtaaa acttcggaca agatggtttg
     1981 agcggatgtt agaatttaaa ccttatattt tcacattttt ttgtttgctt tggcttcaag
     2041 gtatcgccca cgctcaaacg cccgcccaga ctccgcccgc ctccgaaaca gataatacca
     2101 aaacggttca cttcggcgac ctgatagacg tcgatgtcgt cggcagcgcc gaatacgact
     2161 ggcgcggcac catcacgccg gaaggctttt tgagcggcat cgattttgtc gaagaaccga
     2221 tttacgcgct ctgccggacg gaagacgaca tcgcccggca ggttgaaaaa gcttacggca
     2281 aactgctgcg cgagccgaag gtcgtcgttc gcgtgctcga ccgctcgggg cgacccgttt
     2341 cggttttata cggcgcggtc aaaacgccgc agcgttttca gattcagcgc tcggtgcgcc
     2401 tgaacgaatt gattattttg tccggcggca taaccgagcg ggcgagcggc gagattcaga
     2461 tatttcgccc gcaatatctg agctgccagg cggaaaaaaa atcggacgaa aataaaacgg
     2521 cgcttgacga ggaaaaacgc acctcgtttg tctcgacgcg ccgcgaggac aacggctcga
     2581 cttacataaa catcaaaatc gcggatttgc tcgcgggcaa agccgacgcc aatcctttga
     2641 ttttaaccgg cgacattgtg acgattctcg aagccgagcc gatttacatc atcggcggcg
     2701 tggcggctcc gaaacaaatt tcttcgcgtt cgcagataac cgtttcgcgg gcgatagcca
     2761 ccgccggcgg tttgacaaag gaagccgacg gtacgaaaat cgtgattttt cgccgcaacg
     2821 gtcgcgaaaa caaaactatc gaggtcgatt tggacgccgt aaaagcggaa aaggctgaag
     2881 atatagtctt gcaggcgttt gacgtcgtgg atattccgca gaagggaaaa acgaaaagaa
     2941 aatttcctcc ggttattcgt tcaatcgagg gcggcgaaaa aaatgctttg aatttacctt
     3001 tgcgaattgt cgattgattc ggaaaaaggt gacaattcgc aaacgaaaat gtagaattca
     3061 cataacttta gtttttaagg aaaataaatg gcagagaaaa caggtaaata cagccgccgg
     3121 acaatcgctt tattcggttt ggtcgccgtc ggcatcttaa tcgcggtatt ggtcggtttg
     3181 cagcaaatcg ctattttata cgttctggcg acgattagtc tggtcgcatt attattgacg
     3241 gtggcttttg cggatttgga aaaagtcggg cgcgaaaact ccgaaggctt cactccgaga
     3301 agtgaataat taatatttag tgaaaaataa attggcaaaa ttgatttcgg gattattgct
     3361 tgggctgatg atattttctt tgtcggcgcc ggggcttact ccgcagattt cggatgaggc
     3421 taaagccgtg cgtctgaaat ggaaaaccgg aacgattaat atcgcctttt cgtcgtcgct
     3481 gacgaagccg ccgcagaata tcaaatatga gagcgacgtt gtcggcgcgg cgcggcggag
     3541 cctcgcgacg tgggaaaaag ccgccgatat aaaatttaac gaaactttct cggacaaaca
     3601 atccgtctcg cccgccggaa atgccggcga cggcgtgagc cttattacgg tcgcttcgac
     3661 gccggaaaat attcttcttt tcggcgacgg ctcggaaatt tccgccaaaa ccagagtttt
     3721 ttataaccgc cgcggcatta ttaccgaagc cgatattgtt ttgaatccgt atatgcagtt
     3781 ttcgaccgac ggcacgccgg gaacttttga cctcgaagcg acgataaccc acgaaatcgg
     3841 gcatcttctg ggcttggagc attcgtcgct ggtcggttcg acgatgcaga cgcatcaagg
     3901 caaaaacgga atctacaatc tgccgcaatt cgcttcgcga actctctccg acagcgacct
     3961 tgccaatctg cgcgcgcgat acggagcgaa agccgatgaa gattgctgcg gcgcggtaaa
     4021 gggcaaaatc acgcttgcgc cgacggcggc ggcaaataat tttcacgtct ggctcgaaga
     4081 atcggaaacc ggaaaaattc aggcgggaac gattacgcag gcggacggaa cattttatct
     4141 ggacggcttg gaagcgggaa aatatcgcgt tctggcgcag gattcgggcg aaaaacaaaa
     4201 gaatcgaaaa aagatttttt ccggcggcga aataggcgaa gtagttatcg ataaaggaaa
     4261 aactgcttat atcgagaaac aactcgcgct gaaagaaagg tctttcagcg taaaatacat
     4321 cgggtttaac gggcaaatat ccaatattcc gcttccggtc gatggcggaa agtctttcgt
     4381 gatttatctg ggcggcgaaa atctgaatcc gaatgaattg attatttcaa ccggcacgcc
     4441 ttttatcaaa gtcgagaaga atatgaccgt cgcccacgat ttcggcgatg aaatctcagt
     4501 tttaagtttt gaaatatccg ttcagccgaa cgtgccgccg ggagaataca gcctgcggtt
     4561 acaaaaaaat aacggcgaag tcgaatactt tgtcggcggc attattaacg acgcgatatt
     4621 taatcccgcg agcagcccgc ttatcagcga tttcgattaa ctacggagat agaaaaaaat
     4681 atattttcta tcgaatgaag gttgcaaaaa aaaaaatatt gtgcataatc agaattgttc
     4741 acctccccgg tgaacattct ggtcacgagg ggtgaggact tgtgacctaa gggcgacggt
     4801 gtgggagacg tcgc
//
LOCUS       DASWNE010000606         3888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_20513, whole genome shotgun sequence.
ACCESSION   DASWNE010000606 DASWNE010000000
VERSION     DASWNE010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20513"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1414
                     /locus_tag="VGB68_11870"
     CDS             <1..1414
                     /locus_tag="VGB68_11870"
                     /inference="COORDINATES: protein motif:HMM:NF022019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HEY0049980.1"
                     /translation="FLKLRKEGEKTRQENLLLRQRTSKFETTNKNLLEEKQSLSQHLL
                     ESNQKIKEFTDRYQNVIDVEAEKTKVLGEIEKLRQQHLEFSIKYQGDYNAKLDEAKQI
                     EEKIAELQKEFALLDESATLFSFGFYNPRYDFEQSSRYQLELDKIRLAQKNMISGKTA
                     AICSTEWTVNGSRAEGRKQINQTLRLILRAFNGESDAAIAKVKYNNVNVMEARILKAY
                     EALNKLVSIQCVEISRNYLNLKLEELYLVHEYQEKLQEEKEEQRRIREQMREEEIARR
                     EIEKALQDAEREETRYAEALRKAREEVEKAVGDKQTKLLAQIEALQNKLTEVQANKER
                     AISRAQMTRSGHVYIISNIGSFGEDVYKIGMTRRLDPMDRVIELGDASVPFRFDVHAI
                     IYSEDAPALENQLHKKFDDRRINLVNNRKEFFRVSLQEIADVVREHHGEIEFTLAAEA
                     AEYRKTLAFIAERQKQSATI"
     gene            complement(1499..2188)
                     /locus_tag="VGB68_11875"
     CDS             complement(1499..2188)
                     /locus_tag="VGB68_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049981.1"
                     /translation="MAGNRAAVVQPSESLDNAASKTEEKFAAPAPTDEIKRLAVAISD
                     LLDNETLGSKIKVAIINAFGNEDDTSFFCHADLGSPEYNEKWLRHFAGRFYDGEAHLA
                     FQILNILGNEFLPFPIRNGIEKGLADIGQDFNSKADKTESFQYIADYLRYYNAEETDA
                     PKREASNDGDEIQRLANQISELMHNPLLPEKIYNVLTDEFASIPEGLTDTPENILFNL
                     KQMLNKQFPDE"
     gene            complement(2143..2574)
                     /locus_tag="VGB68_11880"
     CDS             complement(2143..2574)
                     /locus_tag="VGB68_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049982.1"
                     /translation="MNNTDNTQKKTTENDYKKAQENYRALLTSLDNPEFAHEFKVRKL
                     DRAKWLLQEIFGVCDRVSVAPDVEDWAQANLMEIFELLLIRHDNFQVNDFVEDLQHLI
                     FENTKSHGAAYRHWSSSLAAERGLDTNWLETEPPSFSRPNL"
     gene            complement(2655..2927)
                     /locus_tag="VGB68_11885"
     CDS             complement(2655..2927)
                     /locus_tag="VGB68_11885"
                     /inference="COORDINATES: protein motif:HMM:NF016330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HEY0049983.1"
                     /translation="MFILKGINQLERAIAKAKQLRPRVEFGGFGHYRVSGSKGGFYTV
                     ICKKSDNGYKLVDCTCKGAEKGFVCYHAAAALAVHTGIARQRQAVA"
     gene            3057..3287
                     /locus_tag="VGB68_11890"
     CDS             3057..3287
                     /locus_tag="VGB68_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0049984.1"
                     /translation="MADKKDKLLSVADVSKDAGISRQRILQIIESGDLRAEMVGRNYV
                     ILQSDFEKWNSSRREAGRPPKDKAEQSKADAK"
     gene            3277..3564
                     /locus_tag="VGB68_11895"
     CDS             3277..3564
                     /locus_tag="VGB68_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049985.1"
                     /translation="MQNKTGWRWNPGDLIGALEACRMLGYKSARQLQDATRRKNIEKE
                     FESVGCTLTLGIWIGGQQRFLRSEIDEFLTKKTEAVQKQDDSRKRNLSLVA"
     gene            3599..>3888
                     /locus_tag="VGB68_11900"
     CDS             3599..>3888
                     /locus_tag="VGB68_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049986.1"
                     /translation="MDKRVKDVIDLLESDEFNCLPLCTNTTHHGTIFPYMFRVYFDPE
                     TPEKGLKVYSFVELALNKFHERIEQNDKFLRLEVAQTFMGLNLPAIVSEVSV"
BASE COUNT         1184 a          798 c          878 g         1028 t
ORIGIN      
        1 ctttttgaag ctcagaaaag aaggcgaaaa gaccaggcag gaaaatctac tcttgcgaca
       61 aagaacatca aagttcgaaa ccactaataa gaatttactt gaagaaaaac aatctctgtc
      121 tcagcatcta ttagaatcga accaaaaaat aaaagaattt accgaccgat accagaatgt
      181 aattgacgtt gaagcggaaa agactaaggt tttaggcgag attgaaaaac tgcgccagca
      241 acatcttgaa ttttccatta aatatcaagg tgactataac gctaaacttg acgaggctaa
      301 acagatagaa gaaaaaatcg ccgaattgca aaaagaattt gctttgctcg acgaatcggc
      361 aactttattt tctttcggtt tttataatcc tcgatacgac ttcgagcaat ccagccgcta
      421 tcagcttgaa ttggacaaaa ttcgtctggc tcaaaagaat atgatttccg gtaaaacggc
      481 agcaatctgt tcgacagaat ggacggtaaa cggaagccgc gccgaaggtc gcaagcagat
      541 taatcaaacg ttgagactta ttttgcgcgc ttttaacggc gaatccgacg ccgctatcgc
      601 caaagtaaaa tataacaatg ttaatgttat ggaagcgcga attttgaaag cttacgaggc
      661 tcttaataaa ttggtcagta ttcaatgcgt cgaaatatcc cgtaactatt taaacttaaa
      721 attggaagag ctttatctgg ttcacgagta tcaggaaaaa cttcaagagg aaaaagaaga
      781 acaacgtcga atccgcgagc agatgcgcga ggaagaaatt gcccggcggg aaatagaaaa
      841 agccttacag gatgccgagc gtgaagaaac ccgctacgcc gaagcattgc gaaaggcgcg
      901 cgaggaagtc gaaaaagccg tcggcgataa acaaaccaag cttttagcgc aaatcgaagc
      961 tctgcaaaat aaacttaccg aagttcaggc aaacaaagaa cgggcaatat cgcgcgcaca
     1021 aatgacccgg tccggtcacg tttatatcat ttccaatatc ggctctttcg gcgaagatgt
     1081 ttataaaata ggaatgacgc gcagacttga cccgatggac agagttattg agcttggcga
     1141 tgcttccgtt ccgttcagat ttgacgttca cgcgattatt tacagtgaag acgcaccagc
     1201 gctggaaaac caactgcaca aaaaatttga cgaccgaaga attaatttag tcaataaccg
     1261 caaagaattt tttcgcgttt cgttacagga aattgctgat gtcgtgcgcg aacatcacgg
     1321 agagatagaa tttactttag cggcagaagc ggctgaatat cgaaaaactt tagcttttat
     1381 tgccgaaagg caaaaacagt cagctactat ataacgagcg aaaaaattca attgtaaaat
     1441 tgaaaagccg cgccggtcgg tcaaaacagg cgcggtggtt tgttgtcggt ttcacggatt
     1501 attcgtcggg aaattgcttg ttaagcattt gtttgagatt gaacaaaata ttttccggcg
     1561 tgtctgttaa accttcggga attgaagcaa attcatcagt caagacgttg taaatctttt
     1621 cgggaagtag cgggttgtgc atcaattccg aaatctgatt cgccaatcgt tgaatctcgt
     1681 cgccgtcgtt gctcgcctcg cgtttcggcg cgtcagtttc ttcggcgttg taatatcgca
     1741 aataatcagc gatatattga aaagattccg ttttgtcggc ttttgaattg aaatcttgtc
     1801 caatgtccgc gagtcctttt tcaattccgt ttctgatagg gaacggcaaa aattcgtttc
     1861 cgagaatgtt caaaatctga aaagccagat gcgcttcgcc gtcgtaaaat cttccggcaa
     1921 aatgacgtaa ccatttttcg ttatattccg gtgagcctaa atcggcgtga caaaaaaaac
     1981 ttgtatcatc ttcatttccg aaagcgttga taatcgcaac cttgattttg cttccgagcg
     2041 tttcattgtc gagcaagtca gaaatcgcaa cggcaagccg tttaatttcg tcggtcggcg
     2101 cgggcgcggc gaatttttct tcggttttgc tcgcggcatt atctaaagat tcggacggct
     2161 gaacgacggc ggctcggttt ccagccaatt cgtatctaaa ccgcgctcgg cggcgagcga
     2221 gctgctccag tgccgatatg ccgcgccgtg tgatttggta ttctcaaaaa tcaaatgctg
     2281 caaatcttcg acaaaatcat tcacttggaa attatcgtga cggattaaaa gcagttcaaa
     2341 gatttccatt aaattagctt gcgcccaatc ttcaacgtct ggcgcgacgg aaacacggtc
     2401 gcaaacgccg aaaatttcct gcaaaagcca tttggcgcgg tctaatttcc tgaccttaaa
     2461 ttcgtgtgca aattcgggat tgtccaaaga tgtgagaagc gcgcggtaat tttcctgcgc
     2521 tttcttgtaa tcgttttcag ttgttttttt ctgtgtgttg tcggtattat tcataattga
     2581 ctgatttcct ttttccaaaa ttggaagtca aagaaggctt tcccgaactg gcggtttagg
     2641 aaagccttcg ccttttacgc tacggcttga cgctggcgag cgatgccggt atgaactgcg
     2701 agcgccgcgg cggcgtgata gcaaacaaaa cctttttccg cgcctttgca ggtgcaatca
     2761 actaatttgt agccgttatc tgatttctta caaatcacgg tataaaatcc gcctttgctt
     2821 cccgaaacac gataatgacc gaagccgccg aactcgacgc gcgggcgaag ctgtttcgct
     2881 ttggcgattg ctctttccag ctgattgatg cctttaagta tgaacattta attatccttt
     2941 ccgagtagaa tttgttaatt gcttaactaa atatatcttg tcatatgaca agatataagt
     3001 caaactaaaa ccttgtttct tgacaagtat tttgaaggaa agttattctt aactgtatgg
     3061 cggataaaaa agataaattg ttgagtgtgg cggacgtaag caaagatgct ggaatttccc
     3121 gacaaagaat tttacagatt attgaaagcg gcgatttacg cgcggaaatg gtcggcagaa
     3181 attacgttat cttgcaatct gattttgaaa aatggaatag ctcccgacgc gaagctggca
     3241 gaccgccgaa agataaagcc gagcaaagca aagcagatgc aaaataaaac cggctggcgc
     3301 tggaatccgg gcgaccttat cggcgcgctc gaagcctgcc ggatgctcgg ctacaaatcc
     3361 gcccggcaac ttcaggacgc aacccgccgg aaaaatattg aaaaggaatt tgaatcggtc
     3421 ggctgcactc tcacgctcgg catctggatt ggcggacagc agcgtttttt gcgaagtgaa
     3481 atcgacgagt ttttgacaaa gaaaaccgaa gccgttcaaa aacaggatga cagtcggaaa
     3541 agaaatttaa gtttagtggc gtgaagttta aggaaaactt tgaaaaagaa aataaaatat
     3601 ggacaaaaga gtaaaagacg taattgattt attagaatca gacgagttta actgtttgcc
     3661 attatgcaca aatacgactc atcacggaac gatttttcct tatatgttta gagtgtattt
     3721 tgaccctgaa acccctgaaa agggcttaaa agtctatagt tttgtggaat tagccttaaa
     3781 taaattccac gaaagaattg aacaaaatga caaatttctt agattggaag tagctcagac
     3841 ttttatgggg cttaacctgc cagccatagt gtccgaagtt tctgttga
//
LOCUS       DASWNE010000607         2783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_90411, whole genome shotgun sequence.
ACCESSION   DASWNE010000607 DASWNE010000000
VERSION     DASWNE010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90411"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1452)
                     /locus_tag="VGB68_11905"
     CDS             complement(<1..1452)
                     /locus_tag="VGB68_11905"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY0049987.1"
                     /translation="MKEEHLMHTPPLFADLLIILLVSVPVAFLCLRLKLPLLVGLMLT
                     GIVIGPYGLGLIKELEAIEILAEIGVMLLLFTIGLEFSLRRLREMKKLVLIGGGLQVG
                     LTIAATAVVAVLFGRGLNQAIFFGFLVALSSTAIVLKSYAERNEIDSPHGRAGVGILL
                     FQDISIVLMMLLVPILGGKDPASFGAIAQTLGGSLLALILIVLGAWFIAPKLIKQVVR
                     LRSPEVLILTVVLLTLGMSWVTSHFGLSLALGAFIAGMVIADSDYSHQVTADILPFRD
                     VFNSLFFVSIGLLLSLGALIENFTIVLLWITLLIAGKSLIIWSVVQLLGFPQRVAAMT
                     ALGLAQIGEFSFVLAKSGRSVDLLPDVDYQSFLAASIISMIATPFMISAAPRFGYFLQ
                     SIFKDQKAGETADDAEEDIHLTSSGGLNNHVIIVGYGLNGRNLARVLRAVLVPYTILE
                     LNADVVRDAKEKGEKINFGDATRREVLHHARIEN"
     gene            complement(1553..1960)
                     /locus_tag="VGB68_11910"
     CDS             complement(1553..1960)
                     /locus_tag="VGB68_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049988.1"
                     /translation="MPYLLKPDSRILFLLRAVLLTGVLAGLLFSCGEGIRLFPFPPAA
                     TQYAQSGWKNGEKVGYQKNALRFEQRQGNYQSKIQRDNQPHYWTDVYNTLHKAPAFAA
                     SPARENAVLFNSPFFKSHLFSISGASRAPPVSS"
     gene            complement(2064..>2783)
                     /locus_tag="VGB68_11915"
     CDS             complement(2064..>2783)
                     /locus_tag="VGB68_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049989.1"
                     /translation="WSCFVGLMYLSFEPYLRKYAPERVISWNRLLAGDWRNPLVGRDI
                     LIGGAAGLAVIVLGSLRYFIPVWFFGEPPLVPHVISNPGGAALLGIRGFPILFLNQIT
                     ASLVFAFMVSFLILFFSLLLRHKWFGYAAFWLLLSSFAVSNDIGAGRPLTGMLWSVLF
                     PTFLVLTAARFGVLAMASGFVFYHLVVFYPITTELSAWYAGDFVLCVIALSALSIYGF
                     YTSLAGQPIFQGSLLPDKEFK"
BASE COUNT          804 a          755 c          734 g          490 t
ORIGIN      
        1 gttttcgatg cgagcgtggt gcaggacttc gcggcgcgtg gcgtcgccga aattgatttt
       61 ctcgcctttt tctttggcgt cgcgcacgac gtcggcattg agttccagaa tcgtgtaagg
      121 cacgagaacg gcgcgcagaa cgcgcgccag attgcgcccg tttaagccgt agccgacgat
      181 gatgacgtga ttgttcaagc cgccgctgga tgtcaggtga atgtcttctt cggcgtcgtc
      241 agccgtttcg cccgctttct ggtctttgaa aatcgactgg aggaaataac cgaagcgcgg
      301 cgcggcggaa atcataaacg gcgtcgcaat catcgagatt atcgaagccg ccagaaagct
      361 ctggtaatcg acgtcgggca gcaaatccac gcttcgaccg gatttcgcca gcacgaacga
      421 aaactcgccg atttgcgcca acccgagagc cgtcatcgcc gccactctct gcggaaatcc
      481 gagaagctgg acgaccgacc agatgattaa gcttttgccg gcgatgagca gagttatcca
      541 gagcagaaca atcgtaaaat tttctataag cgcgcccagc gaaagcagca gcccgatgga
      601 aacgaaaaac aggctgttga aaacgtcccg gaaaggcaga atgtcagccg tcacctgatg
      661 gctgtaatcc gaatcggcga tgaccatccc ggcgataaac gctcccaacg ccagcgaaag
      721 cccgaaatgc gaagtcaccc acgacattcc gagcgtcagc agaacgaccg tcagaattaa
      781 aacttccggg ctgcgcagtc tgacgacctg tttaatcagc ttcggagcga tgaaccacgc
      841 gccgagcaca atcagaatca aagccagcag agagccgccg agcgtttggg cgatagcgcc
      901 gaaagacgcc gggtcttttc cgcccagaat cggaaccaaa agcatcatca aaacgatgct
      961 gatgtcctga aaaagcaaaa ttccgacgcc cgcgcgaccg tgcggcgagt cgatttcgtt
     1021 gcgctcggcg tagcttttca acacaatcgc cgtgctcgaa agcgcgacca gaaacccgaa
     1081 aaaaatcgcc tgattcaatc cgcgcccgaa aagaacggcg acgaccgcgg tcgccgcaat
     1141 cgtcaacccg acctgcaagc cgccgccgat aaggacgagc tttttcatct cgcgcaggcg
     1201 gcgcagcgaa aattccagcc cgatggtgaa aagaagaagc atcacgccga tttcggcgag
     1261 aatctcgatt gcttccagct ctttgataag tcctaaaccg taaggtccga tgacgattcc
     1321 ggtcagcatc aaaccgacca gaagcggcag cttcagacgc agacagagaa aggcgaccgg
     1381 cacggagacc agcaaaataa ttaataaatc ggcaaataaa ggcggcgtgt gcatcagatg
     1441 ttcctctttc acgctcctga cgtgttttta aacgaataca aatcaagcga tgagagcctt
     1501 cgattaagaa aaaagccctg cttttcaaaa aactgcgatg atttataaaa gcctaagagg
     1561 aaacgggagg agcgcggctc gcgccggaga tggaaaaaag atgcgatttg aaaaatggtg
     1621 aattgaataa aacggcgttt tcgcgggcgg gcgacgcggc aaaagccggc gctttatgca
     1681 gcgtgttata aacatccgtc caataatgcg gctgattatc gcgctgaatt ttcgattgat
     1741 aattgccctg ccgctgctcg aagcgaagag cgtttttctg atagccgacc ttctcgccgt
     1801 ttttccagcc ggattgcgca tactgcgtcg ctgcgggcgg aaacgggaaa agcctgatgc
     1861 cttcgccgca cgaaaacaaa agtcccgcca gcacgccggt taataaaacg gcgcgcagca
     1921 ggaaaagaat ccggctatct ggctttaaaa gatacggcat cgcttatttt aagcataaca
     1981 agcgaatggt gaatcaacaa acaaaagttt ttaaccgaag aactgagaaa cgagaattcg
     2041 gcgcggctcg gaattctggt ttctcactta aattccttgt ccggcagcag gcttccctga
     2101 aaaatcggct gtccggcaag cgacgtgtaa aatccgtaaa tcgacaatgc cgacagagcg
     2161 atgacgcaca aaacgaaatc gcccgcatac cacgccgaaa gctcggtcgt aatcggatag
     2221 aaaacaacga gatgataaaa aacgaaaccg gaagccattg ccagaacgcc gaaacgcgcc
     2281 gccgtcaaaa ccagaaacgt cgggaacagc accgaccaga gcatcccggt caaaggtctg
     2341 cccgcaccga tgtcgtttga gaccgcaaag ctcgaaagca acagccagaa agcggcgtag
     2401 ccgaaccatt tgtgccgcaa aagcagcgaa aaaaacaaaa tcaaaaacga aaccataaag
     2461 gcgaagacga gcgaagcggt gatttgattc aaaaacaaaa tcggaaagcc gcgaatgccc
     2521 agcagagccg cgccgccggg attcgagatg acgtgcggaa ccagcggcgg ctcgccgaaa
     2581 aaccagacgg gaatgaaata gcgcaggctg cccaaaacta tcacggctaa accggcagcg
     2641 ccgccgatta aaatatcgcg cccgaccagc gggttgcgcc agtcgccagc cagcaggcga
     2701 ttccaggaga taacgcgttc gggcgcgtat tttctcaggt aaggctcgaa tgataaatac
     2761 atcagcccga caaaacacga cca
//
LOCUS       DASWNE010000608         3610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_402682, whole genome shotgun sequence.
ACCESSION   DASWNE010000608 DASWNE010000000
VERSION     DASWNE010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402682"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="VGB68_11920"
     CDS             complement(<1..427)
                     /locus_tag="VGB68_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049990.1"
                     /translation="MTEKWFESSWANATPLIIFIIFMIVFLAAQAVLDVCDESNSLFC
                     SSIDIKNHILFAPQKVFTETDGKTGLLKDKDKEGKETETYAASRKIMANRYSGQIVWI
                     FFVGINFFISIVGLTIYLLLAVKTKKYKYVAGGLIISLVS"
     gene            complement(544..1203)
                     /locus_tag="VGB68_11925"
     CDS             complement(544..1203)
                     /locus_tag="VGB68_11925"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0049991.1"
                     /translation="MAAQSDNSNGKNSEGLSLHTGEGVVIDIGTGDGRFVSAAARANP
                     NRFYIGIDANAKPLEKISMKATRKPSKGGLANVLFVQAAVENLPEELNASADEIHIHF
                     PWGSLLRAVASGDEKVLESLRRLGAPECLVEIIIGIDEERDKSEIERLALPKLSSDYL
                     KSALIPKYEAAGFKLLESGILNSSEWSKLETSWARKLQGGSNRKVIYLILQINQSSAS
                     K"
     gene            1295..1993
                     /locus_tag="VGB68_11930"
     CDS             1295..1993
                     /locus_tag="VGB68_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEY0049992.1"
                     /translation="MKRCPQCNNLYPNETQYCLNDGQLLISEVLPLPSQLSVDDEEAT
                     IIRNEPIVVDLSAANEPGTEALPHRIPPPIEKIPVRAKSNFGKYLVFLITGLLLGGGL
                     VLATLFLSRSFYQNNNAAERGATANVNSNQTRGNSEPKNTDSPVDKPITAKPEHQERT
                     DVGDDEFNGRVITLNAYVRSSPSRTSPEIEILPIDDRLTITSRDNPNSPWFHVVCEHG
                     TSGWMHGNTIEYTR"
     gene            complement(2148..3176)
                     /locus_tag="VGB68_11935"
     CDS             complement(2148..3176)
                     /locus_tag="VGB68_11935"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY0049993.1"
                     /translation="MQEETQIFENPDFETQQQQPLQQRKALTYTPSTVAHILNELRKI
                     VVGQDEVIEQILVAVLAEGHALLEGVPGTAKTLTVKTLARIIGAEFSRIQFTPDLMPS
                     DITGTNVFNVRTSEFNLRQGPIFTDILLADEINRTPPKTQSALLEAMEERQTTIDGDR
                     HPLSPLFTVLATENPIEYEGTYPLPEAQLDRFLLKILIDYPSQAAEQEIVARWDSGFN
                     SRHLEQIDILPLGDVTAIQHCRLEVKQMKTEPGVQKYIVEIVHRTRNHPNILYGASPR
                     ASVALLLCAKALAAIRGRDFSTPDDVRDVAMPVLRHRLALRAEAELDGATTDAVISDI
                     IKTVEVPR"
     gene            complement(3189..>3610)
                     /locus_tag="VGB68_11940"
     CDS             complement(3189..>3610)
                     /locus_tag="VGB68_11940"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY0049994.1"
                     /translation="TLWKRDAGVAKAENISPNTIFLAYVLTFLLGVYGGLYSGGYVTI
                     LTACFVAFFGMTFTEAVANTKLINVFSSAAASAVFAYQGLIDYKIGIILAVTMFCGAY
                     IGAHVVTKLNDVWIKRIFIATVLILAVKTIFDFVVVQ"
BASE COUNT          889 a          928 c          901 g          892 t
ORIGIN      
        1 cggaaacgag cgaaataatt aaaccgcccg cgacatactt atattttttg gtcttgacgg
       61 ctaaaagcag atagatggtc aagccgacaa tgctgatgaa aaagtttatt ccgacgaaaa
      121 atatccagac aatctgcccg ctgtagcggt tagccattat tttacgactg gcggcgtaag
      181 tttccgtctc ttttccttct ttatccttat ctttgagcaa accggtcttg ccgtcggttt
      241 cggtaaaaac tttttgcggc gcgaaaagaa tatgattttt gatgtcaatc gacgagcaga
      301 ataatgaatt tgattcgtcg cagacatcca gaaccgcctg cgccgccaaa aaaacaatca
      361 taaaaataat gaatattatt aaaggcgtcg catttgccca gctgctttca aaccatttct
      421 ccgtcatagg cgtaaaacct tccgctgcgc taaaagtgaa tttattcagc gtctttataa
      481 cacgagacgc cccggtcgag caaagctttt ttcgcgtccg agcgcgcctt ttatttacaa
      541 cttttatttt gacgcgcttg attgattaat ttgcagaata agataaataa ctttgcggtt
      601 tgagccgccc tgaagctttc gcgcccacga ggtttcgagc tttgaccatt cggaggaatt
      661 taaaatgccg ctttccagca gtttgaaacc ggcggcttcg tatttcggaa tcaaagcgct
      721 tttaagataa tcggaagaaa gcttcggcag cgcgagccgc tcgatttcgg atttgtcgcg
      781 ttcttcgtca atgccgataa ttatttcaac caggcattcg ggcgcgccga ggcggcgcag
      841 gctttcgagc accttttcat cgcctgaagc gacggcgcgc agaaggctgc cccaggggaa
      901 atggatgtga atctcgtccg ccgacgcatt taattcttcg ggaagatttt cgaccgccgc
      961 ctggacgaac agaacattag cgagcccgcc tttcgagggt ttgcgcgtcg ccttcatcga
     1021 gattttctcc agcggcttcg cgttggcatc tatcccgata taaaacctgt tcgggttggc
     1081 tcgcgccgcc gccgatacaa agcgaccgtc gcccgtcccg atgtctatca cgacaccttc
     1141 gccggtatgg agcgataagc cttcggaatt cttcccgttc gagttatcag attgtgctgc
     1201 cacgaattgc tttaatcata tcttttgcct ttccgttctg gcattataat gttggacgaa
     1261 cggaacggca aggatataaa ttttaaattg ttttatgaaa aggtgtccgc aatgcaacaa
     1321 tctctacccg aacgaaacgc agtattgtct gaacgacggt cagcttttga tttcggaggt
     1381 tttgccgctg ccttcgcagt taagcgtcga tgacgaagaa gcgacaatca ttcggaacga
     1441 accgattgtc gtcgatttat cggcggcaaa tgagccggga acggaagcgc tgccgcaccg
     1501 gattccgccg ccgattgaaa aaattccggt tcgagccaaa tcgaatttcg gaaaatatct
     1561 cgttttcctg ataaccggtt tgctgctcgg cggcggattg gttttagcga cgctgtttct
     1621 atcaagaagc ttttaccaaa ataacaatgc cgccgagagg ggagcgactg caaacgtaaa
     1681 ttccaatcaa acgcgcggca acagcgagcc gaaaaacacc gattcgcccg tcgataaacc
     1741 tataacggca aaacccgaac atcaggaaag aaccgatgtc ggcgacgatg aatttaacgg
     1801 tcgcgtgata acgctcaacg cctacgttcg ctcgtcgccg agccgcacgt cgccggaaat
     1861 cgaaattctg ccgattgacg accgcctgac catcaccagc cgcgacaatc cgaacagccc
     1921 gtggtttcac gtcgtctgcg agcacggcac cagcggttgg atgcacggca atacgattga
     1981 atacacgcgg taaattcggg attcaggatt caggatttaa gattcaggat ttaagattca
     2041 ggattcgggg attcgggatt caggattcaa gattcgggat tcaaaattaa aatttgcatt
     2101 caaaatctta aatcctgaat cctgaatcct gaatcccgaa tccctgatta tcgcggaact
     2161 tcgacggttt tgataatgtc ggaaatcacg gcgtcggtcg tcgcgccgtc gagctcggct
     2221 tcggcgcgca gcgcaaggcg gtgacgcaag accggcatcg ccacgtcgcg cacgtcgtcg
     2281 ggcgtcgaaa aatcgcgccc gcgaatcgcc gccagagcct tcgcgcacaa aagcagcgcg
     2341 accgatgcgc gcgggcttgc gccgtagaga atgttcgggt gattgcgcgt tcgatgaacg
     2401 atttcgacga tgtatttctg cacgcccggc tcggttttca tctgtttgac ttccagccgg
     2461 caatgctgaa tcgccgtcac gtcgccgagc ggcagaatgt cgatttgctc cagatgccgc
     2521 gagttaaatc ctgagtccca gcgcgcgacg atttcctgtt cggcggcttg cgaagggtaa
     2581 tcaatcagaa ttttcagcaa aaatctatcc aactgcgctt ccggcagcgg ataagttccc
     2641 tcgtattcaa tcgggttttc ggtcgccaga accgtgaaca gcggcgagag cggatgacgg
     2701 tcgccgtcaa tcgtcgtctg gcgttcttcc atcgcttcca aaagcgcgga ttgggttttc
     2761 ggcggcgtac gattgatttc gtccgccagc agaatgtccg tgaaaatcgg accttgccgc
     2821 aggttgaatt cggacgtgcg aacgttgaaa acattcgttc ccgtaatgtc ggacggcatc
     2881 aaatcgggcg taaactgaat gcgcgaaaac tcggcgccga tgattcgcgc cagcgttttc
     2941 acggtcaggg ttttcgccgt tcccggcacg ccttccaaaa gcgcgtgacc ttccgccaga
     3001 acggcgacca gaatctgctc gataacttcg tcctgcccga cgacgatttt ccgaagctcg
     3061 tttaaaatgt gcgccacggt cgacggcgtg taggtcagcg cctttctctg ttgcagcggc
     3121 tgttgctgct gggtttcaaa gtccgggttt tcgaatattt gtgtttcttc ttgcattttt
     3181 aagtttattt attgtacgac gacaaaatcg aaaattgttt tgacggctaa aatcaaaacc
     3241 gtcgcgataa aaattcgttt gatccaaacg tcgtttagtt tggtgacgac gtgcgcgccg
     3301 atgtaagctc cgcaaaacat cgtcacggct aagataatgc cgattttata atcaatcaaa
     3361 ccctgataag caaaaaccgc gctcgccgct gcggacgaaa aaacgttgat aagttttgtg
     3421 ttcgcaaccg cttcggtaaa cgtcatcccg aaaaaagcga cgaaacaagc cgttaatatc
     3481 gttacgtagc cgccgctgta caagccgccg taaacgccga gcagaaacgt caaaacgtaa
     3541 gccagaaaaa tcgtattcgg cgaaatattt tcggctttgg cgacgcccgc gtcgcgtttc
     3601 cagagcgtaa
//
LOCUS       DASWNE010000609         3178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_90465, whole genome shotgun sequence.
ACCESSION   DASWNE010000609 DASWNE010000000
VERSION     DASWNE010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90465"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VGB68_11945"
     CDS             complement(<1..379)
                     /locus_tag="VGB68_11945"
                     /inference="COORDINATES: protein motif:HMM:NF019187.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049995.1"
                     /translation="MFEFFTNIKVDWMGKRRVLTAISVVILLAGLISAIGRQAVDGGT
                     NAFNLGVDFQGGTVVTAKFRQKPAADDIRAALQTAGVNDAVIQESTDKQDEVLIKVPI
                     IEGQELPAATTPEGQPIDNSQVNT"
     gene            complement(474..2147)
                     /gene="secD"
                     /locus_tag="VGB68_11950"
     CDS             complement(474..2147)
                     /gene="secD"
                     /locus_tag="VGB68_11950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEY0049996.1"
                     /translation="MKNRTLLFRTAIIVVITLIALYLVFGPRQSFTANDFSWSGIKNN
                     LAGNINLGLDLKGGSHLVMRVKVEEYLKTLTENNGLAAQTVAKQANLPVGEPKFVTAN
                     GTYQITLPVTDAAQKQAVVDAVKPKVDFSLWTQSETNDAIVWSLPNTAQRTLSDQAVE
                     QALRIIDGRINSFGVKEPTLARQGGQDSQQILLQMPGVEDPERVKKLIGETAQLSLMK
                     VVSPPNPAPVQTYPTREAALQSIGGQESVTRKVFPYADRDETATDPQNPQPTSFVVVE
                     SPAIVDGSELREATAYSQGGDRDYKIAFALKPAGAAKFGDWTAKNINSYMAVVLNEQV
                     KSAAFIKSQISDSGEISGRFTKESADDLALTLKSGALPAPIEYQEERTVGPSLGADSI
                     RAGVTASVAGLIFIVIFMLIYYRGSGINAVIALILNMLLTLAALIMFDSTLTLPGIAG
                     LILGIGMAVDSNVLIFERIREELRAGKSVAQAVELGFDRAFITIIDTHITTIISSVIL
                     YMYGSGPIRGFAVTLILGLLINLFSAVFVSRTIFMWLLDKKQDMKKLSI"
     gene            complement(2210..2512)
                     /gene="yajC"
                     /locus_tag="VGB68_11955"
     CDS             complement(2210..2512)
                     /gene="yajC"
                     /locus_tag="VGB68_11955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEY0049997.1"
                     /translation="MNIVALFFQGEGGSFIWTLLPFVFIFGIFYFLVIMPQRRQAQQL
                     QEMISSLKINDEVVTNGGIIGKIKEIRETSFIILSADKTFLEIGKSAVVGKKAESK"
     gene            complement(2613..>3178)
                     /locus_tag="VGB68_11960"
     CDS             complement(2613..>3178)
                     /locus_tag="VGB68_11960"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEY0049998.1"
                     /translation="GAGHLELRRESLDKTVEIGFDGYAIGGLSVGEEKSVMYDVIEFI
                     APQMPVDAPRYLMGVGTPEDLVEAVYRGVDMFDCVIPTRNGRTGSAFTTRGKINIRNA
                     RFAKDGAPLDVDCPCSVCRRYSRAYLRHLYQAGEMLAAIMISHHNLAFFLDTMRKVRD
                     SITLGSFGRFRHDFLNKIREGEAERQS"
BASE COUNT          775 a          869 c          768 g          766 t
ORIGIN      
        1 ccgtgttgac ctgactgtta tcaatcggtt gtccttccgg agtcgtcgcg gcgggcagtt
       61 cctgtccttc gataatcgga actttaatca ggacttcgtc ctgtttatcg gtcgattcct
      121 gaataaccgc gtcgttgacg ccggcagtct gcaaagcggc gcgaatgtcg tcggcagcag
      181 gcttctgcct gaatttcgcc gtgacgaccg ttccgccctg aaaatcgacg cccaggttaa
      241 acgcgttggt tccgccgtca accgcctgac gaccgatcgc cgaaatcaaa ccggcgagaa
      301 gaattacaac ggaaatcgca gtcagaactc tgcgcttccc catccagtca acttttatat
      361 tcgtaaaaaa ttcaaacatt tttttagagt tacggaaagt tgcggaaaat tgcggaaagc
      421 tgcggaaagt tcaaaactct gaaacttccg caactatctg caactgcctg caactaaata
      481 cttaatttct tcatatcctg ttttttgtcc agcagccaca taaaaatcgt gcgcgagaca
      541 aaaaccgccg agaaaaggtt gattaaaagt ccgagaataa gcgtgacggc aaatccgcga
      601 atcggacccg aaccgtacat atacaatatg acggacgaga taatcgtcgt aatgtgcgta
      661 tcgataatcg tgataaacgc ccggtcaaat cccaattcga cggcttgcgc gaccgatttt
      721 ccggcgcgca gctcttcgcg gatgcgctca aagataagca cgttcgaatc gaccgccata
      781 ccgatgccga gaatcaaacc ggcgatgccg ggcagcgtca gcgtcgaatc gaacataatc
      841 aaagccgcca gcgtcagaag catattcaaa attaaagcga tgacggcgtt aatgccggaa
      901 ccgcgataat agatgagcat aaaaatgacg ataaaaatca aaccggcgac ggacgccgtc
      961 acgcccgcgc gaatcgaatc cgcgccgagg ctcggtccga cggttctttc ttcctggtat
     1021 tcaatcggcg caggcaaggc tccggatttc aaagtcaaag ccaaatcgtc ggctgattct
     1081 ttggtaaagc gtccggaaat ttcgcccgaa tcggaaatct gcgatttgat aaacgcggct
     1141 gatttaacct gttcgttgag cacgaccgcc atataactgt tgatattttt ggctgtccag
     1201 tcgccgaatt tagctgcgcc cgccggtttc agcgcaaacg caatcttgta atcgcggtcg
     1261 ccgccctgcg aataagcggt cgcttcgcgc agctcgctgc cgtcgacaat cgccggactc
     1321 tcgacgacga caaagctggt cggctgcgga ttttgcgggt cggtcgccgt ttcgtcccgg
     1381 tcggcatacg ggaaaacttt gcgcgtcacg ctttcctgtc cgccgattga ctgaagcgcg
     1441 gcttctctgg tcggatacgt ctggacgggc gccgggttgg gcgggctgac gactttcatc
     1501 agcgacaact gagccgtctc gccgattaat tttttcactc tttccgggtc ttcgacgccg
     1561 ggcatctgca aaagaatctg ctgcgaatcc tgtccgccct gtcgcgccag agtcggttct
     1621 ttgacgccga aagaattgat tcgaccgtcg atgattcgca aagcctgttc gaccgcctgg
     1681 tcgctcaaag ttcgctgcgc cgtattcggc aggctccaga caatcgcgtc gtttgtctcc
     1741 gactgcgtcc agagcgaaaa atcgactttc ggcttgaccg cgtcaacgac cgcctgtttt
     1801 tgagcggcgt cggtcaccgg caaagtaatc tggtaagtcc cgtttgccgt gacgaattta
     1861 ggttcgccga ccggcagatt cgcctgcttg gcgacggttt gagcggcaag cccgttgttt
     1921 tcggtcagag ttttcagata ctcttcgacc ttaacgcgca tcaccagatg cgagccgccc
     1981 ttcaaatcca gaccgagatt gatatttccg gctaagttat tcttaatgcc cgaccatgaa
     2041 aagtcgttcg cggtgaacga ctgacgcgga ccgaaaacca aatacaacgc aatcagtgtg
     2101 atgactacga taattgcagt tctaaacaat aatgttctgt ttttcattac tactcgttag
     2161 tagtcagtcg cccgtcgccg gttgtccgtc gccgagccga atttgctgac tatttgcttt
     2221 ccgctttttt tccgaccacg gcgcttttgc cgatttccag aaatgttttg tctgcgctca
     2281 ggatgataaa gctggtttct ctgatttcct tgattttgcc gataatgccg ccgttggtga
     2341 cgacttcatc gttgattttc aggctggaaa tcatctcctg caattgttgc gcctgacggc
     2401 gttgcggcat aatcacgaga aaatagaaaa tgccgaagat aaatacgaac ggcaaaagcg
     2461 tccaaatgaa acttccaccc tcaccttgaa aaaataaagc tacaatgttc attgttttaa
     2521 tgcagacaga aacttcaatt ataaattacg caagacgaat aataaaaccg ccgaagctaa
     2581 aaacagcgat ttattatgcg gaaacggcaa aattatgatt gcctttccgc ctcgccctca
     2641 cgaattttat ttaagaagtc gtgtcgaaac cgtccgaagc tgccaagtgt gatagaatct
     2701 ctcaccttgc gcattgtgtc aaggaagaac gccagattgt ggtgcgaaat cataatcgcc
     2761 gccagcatct cgcccgcctg atacaaatgc cgcagatacg cccgcgaata acgacggcaa
     2821 accgaacaag gacaatcgac gtcgagcggc gcgccgtctt tggcaaatct cgcgttgcga
     2881 atattgattt tgccgcgcgt tgtaaaagcg cttcccgtcc gcccgttgcg cgtcggaatc
     2941 acgcagtcaa acatatcgac gccgcgataa accgcttcga ccaaatcttc gggcgtgccg
     3001 acgcccatca gatatctggg cgcatcgacc ggcatctgcg gcgcgataaa ctcgataacg
     3061 tcatacatca ccgatttttc ttcgccgacg ctcaagccgc cgatggcgta gccgtcaaag
     3121 ccgatttcaa ctgttttatc cagactttct cttcttaatt ccagatgccc cgcgccct
//
LOCUS       DASWNE010000610         4124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_585809, whole genome shotgun sequence.
ACCESSION   DASWNE010000610 DASWNE010000000
VERSION     DASWNE010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585809"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VGB68_11965"
     CDS             <1..385
                     /locus_tag="VGB68_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0049999.1"
                     /translation="CAIALEAQLKFQAPGITRQLGLGIGKLVFTALNRVECVLAILLA
                     IALFAFAGARRRARIVFGVVAAILLAQTFWLIPLLVERIDLITSGQTPQGSSAHLVYI
                     AFEVAKILLLLTLSVLLNLPDKSEK"
     gene            382..774
                     /locus_tag="VGB68_11970"
     CDS             382..774
                     /locus_tag="VGB68_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HEY0050000.1"
                     /translation="MKKDIENRADLELLLTEFYKIATADAEIGHHFAGLDLAAHLPVI
                     VDFWEKILFGKQVYFGNPLFVHQKINEKSPLELEHFRRWVEIFSQTVDKLFAGETAEN
                     ARLRARMIAHSLHQRIHEQDFPGVRVSR"
     gene            1037..2536
                     /locus_tag="VGB68_11975"
     CDS             1037..2536
                     /locus_tag="VGB68_11975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY0050001.1"
                     /translation="MHSKEKNQKIFFADKFSLLARLGIGILSILFLSLIISRLAFPFD
                     NGILEAFNWLPAQHILEGKNPYAFALVPPYSMTPYGAIFYALLAVGQKLFGFQLWWGR
                     ILSVLAFAFCLWAIVKITKKITLEREAARFAFLTGLAMFPAQFWIGAIRPDLIALAFV
                     AGALWLVFTRVEENKKTPLWIIAAIILLSSAAFFTKQTFFFTAGIAFLRFLQLGKLRE
                     AVLTASAFAALAAAGIFLLNYTSDGGYVWQHWTHAQRLTFSWRQVIDEFLKTLKTPVF
                     LLALIFLFIFIYRKQKQLFPASRAKLLEALRSPELLILFYFLISLGWAFLSCGRVGAN
                     VNYYIENSLLLAIIGGFIYADFKRDFSPKLALAMIVLFTLGGASQLARILHGEYFRWQ
                     AVGYYHEISDRTAKTIPPGGTCISVAAELVVRGGCRFHFDDFSEYEHGWSPELREAFE
                     REVKTGRYAAILWYDNALQTRFPNYRLVPTSQNAPERSFPVYLYVPTNP"
     gene            2639..3406
                     /locus_tag="VGB68_11980"
     CDS             2639..3406
                     /locus_tag="VGB68_11980"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0050002.1"
                     /translation="MLNKNSAPGISVFFPAYNDAKSIGKLVADALEILPDLTDDYEII
                     VVNDGSTDETGEVLRRLEAKHKEVRIVEHETNKGYGGALQSGFRAATKDLIFYTDGDG
                     QYDARELAKLYALLNEKTDVVNGYKLERGDKINRKVVGGFYNRLAHLLFSLPIRDIDC
                     DFRLIRKEFSDKIALSSTSGSICVELIYKLNRAGARFRETGVNHYERRFGKSQFFTVR
                     RVTKTLYDFFSLWLKLVVLRKNSENLVLFALIISEHL"
     gene            3410..>4124
                     /locus_tag="VGB68_11985"
     CDS             3410..>4124
                     /locus_tag="VGB68_11985"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0050003.1"
                     /translation="MGISPEKYKGRSVLITGGLGFIGSNLARRLAELGDVDITIIDSL
                     SEDQGGSLYNIEDFKDRVKLHVADMGDSWVINHLVGGVDYIFNLAGSISHLDSMRTPQ
                     RDLQLNCAIHLTLLEACRMFNPHVKIVFTSTRQVYGKPAYLPIDEQHRVQPMDINGIN
                     KYAAEMYHLLYQRAYGLRTVNLRLTNTYGPRQQMYHNRQGFIAWFIRQAMDGETIELF
                     GDGRQSRDLNHVDDVVDALL"
BASE COUNT          935 a          981 c         1086 g         1122 t
ORIGIN      
        1 ttgcgcgatt gccttggaag cgcaactgaa atttcaagcg ccgggaatta cgcgccagct
       61 cggactcggc atcggcaagc tggtttttac ggctctgaac cgcgtcgaat gcgtgctggc
      121 gattctgctg gcaatcgcgc ttttcgcctt tgccggagcg cggcggcggg cgagaatcgt
      181 tttcggcgtc gttgccgcaa ttttgctggc gcagactttc tggctgattc cgcttttggt
      241 cgaaagaata gatttaataa cgagcgggca aacgccgcag ggttcgtcgg cgcacctcgt
      301 ttatatcgct tttgaagtcg ctaaaatttt gcttttattg actttaagcg ttttgctgaa
      361 tctgccggac aaatctgaaa aatgaaaaag gatatagaaa atcgcgcgga tttggagctg
      421 cttttaacgg agttttataa aatcgccacc gcggacgcgg aaatcgggca tcattttgcc
      481 ggattggatt tagccgcgca tttgccggtt atcgtcgatt tctgggaaaa aatcctgttc
      541 ggcaaacagg tttatttcgg caatccgctt ttcgttcacc agaaaataaa cgaaaaatcg
      601 ccgctcgaat tagagcattt tcgccgctgg gtagaaattt tcagccaaac cgtcgataag
      661 cttttcgccg gcgagacggc ggaaaacgcc aggctccgcg cccggatgat tgcgcacagt
      721 cttcatcaaa gaattcacga gcaggatttt cccggcgtgc gagtttcgag ataaaacttt
      781 acccgccgaa gcgaatagag cgaaacagag aaatcagccg cagataaacg cggataacgc
      841 ggatgagaaa tgccttttat caagctgcgc ggctgaccgg attaagccgc gaagcctgct
      901 tttaatctgc gtttaccggc gaaaatctgc ggtttcattt cccggcggct aatttatgcg
      961 gttgtgaaat gctcgaacgt gtgggaaaat ctccgcaacc ttttaaaacg gacgaagctt
     1021 tcataaaaaa ctcaaaatgc attcaaagga aaaaaatcaa aagatttttt ttgcagataa
     1081 attttcgctt ctggcgcggc tcggaatcgg gattttgagc attttatttt tgtctctgat
     1141 aatttcgcgg ctcgcttttc cttttgataa cggcattctc gaagcgttta actggctgcc
     1201 ggcgcagcac attctggaag gcaaaaaccc atacgctttc gctctcgtgc cgccatacag
     1261 tatgacgcct tacggggcga ttttttacgc tttgcttgcc gtcgggcaaa agcttttcgg
     1321 atttcagctc tggtggggaa gaattttatc cgttttggct ttcgcctttt gcctgtgggc
     1381 gattgtaaaa atcacaaaaa aaataacgct cgaaagggaa gccgcccggt ttgctttttt
     1441 gacggggctg gcgatgtttc cggcgcaatt ctggattggc gcgattcgac ccgatttgat
     1501 tgctttggct ttcgtcgcgg gcgctttatg gctggttttt acgcgggtcg aagaaaataa
     1561 aaaaacgcct ctttggatta tcgccgcaat cattttatta tcgtcggctg cttttttcac
     1621 caagcagacg ttttttttta ccgccggaat cgcttttttg cggtttctgc agctgggaaa
     1681 actacgcgaa gccgttttga ccgcttcagc ttttgcggct ttggcggcgg ctggaatttt
     1741 tttgctgaat tacacctctg acggcggcta cgtctggcag cactggacgc acgcgcagcg
     1801 gctgactttc agctggcgtc aggtgataga cgaatttctg aaaacgctca aaacgccggt
     1861 gtttctcctg gcgctcattt ttctgtttat ttttatttac cggaaacaaa aacagctttt
     1921 tccggcaagc cgcgcaaagc tgcttgaagc tttgcgctcg ccggaattgt taattttgtt
     1981 ttactttttg atttctctcg gctgggcttt tctctcctgc ggcagagtcg gggcaaacgt
     2041 caattattat atcgaaaact cgcttctgct ggcgattatc ggcggattta tctatgcgga
     2101 ttttaaacgc gatttttcgc cgaagctcgc tctggcgatg attgttttgt tcacgctggg
     2161 cggcgcttct cagctggcgc ggattctgca cggcgagtat tttcgatggc aggcggtcgg
     2221 ctattaccac gaaatttcag accgcacggc gaagacgatt ccgccgggcg gaacctgcat
     2281 cagcgtcgcc gccgaattgg tcgtccgcgg cggctgtcgt ttccattttg acgatttcag
     2341 cgaatacgag cacggctggt cgccggagct gcgcgaggct ttcgagagag aagtaaaaac
     2401 gggtcgctac gcggcgattt tgtggtatga taatgccttg caaacacgat ttccgaatta
     2461 tcgtttagtg cccacttcgc aaaacgcgcc ggaacgctct ttcccagttt atctctacgt
     2521 tccgacgaat ccttaatttc gggttataga atttgaagtt tttttatttc aggcttttgc
     2581 cgcgcgagtc gtaagcgcgg gtttctgttt tcgcgccttt ggcgggaaat atttttctat
     2641 gttaaataaa aattcggctc ccggtatcag cgtgtttttt ccggcttata acgatgcgaa
     2701 atcaatcgga aaactggtcg ccgacgcgct ggagattttg ccggatttaa cggacgatta
     2761 cgagattatc gtcgtcaatg acggaagcac cgacgagacg ggcgaagttt tgcgccggct
     2821 ggaagccaaa cataaagaag tgcgcatcgt cgagcacgaa acgaataaag gttacggcgg
     2881 cgcgctgcaa agcggttttc gggcggcgac caaggatttg attttttata ccgacggcga
     2941 cgggcaatac gacgcccgcg agctcgccaa gctttacgct ttgctcaacg aaaaaacgga
     3001 cgtggtaaac ggctacaagc tggagcgcgg cgacaaaata aaccgcaaag tcgtcggcgg
     3061 tttttacaat cgtctggcgc atttgctgtt ttctttgccg attcgtgaca ttgactgcga
     3121 ttttcgcttg attcgcaagg agttttcgga caaaatagcg ctatcttcaa caagcggtag
     3181 catttgtgtc gagctgattt ataaacttaa tcgcgccgga gcgcgttttc gcgaaaccgg
     3241 cgttaatcat tatgagcgcc ggttcggtaa gtcgcagttt tttaccgtgc ggcgcgtgac
     3301 aaaaacttta tacgattttt tttctctgtg gctgaagctc gtcgttttgc gaaaaaacag
     3361 tgaaaatctg gtgttattcg cgttgattat ttcggagcac ttgtaagtta tgggcatttc
     3421 tcccgaaaaa tataaaggtc gctcggtttt gataaccggc ggattgggct tcatcggcag
     3481 taatctggcg cgacgtctgg ctgagctcgg cgatgtcgac attaccatta tcgactcgct
     3541 cagcgaagac cagggcggaa gcttatacaa catcgaagac tttaaagacc gcgttaagct
     3601 gcacgtcgcc gatatgggcg acagctgggt gataaatcat ctggtcggcg gggtcgatta
     3661 tatttttaat ctggcgggca gcatcagtca tctcgattcg atgcgcacgc cgcagcgcga
     3721 tttgcagctc aactgcgcta tacacctgac tttactggaa gcctgccgga tgtttaaccc
     3781 gcacgtcaaa atcgttttca ccagcacgcg tcaggtttac ggcaaacccg cgtatctgcc
     3841 gattgacgag cagcatcgcg tccagccgat ggacatcaac ggcatcaata aatacgcggc
     3901 ggaaatgtat cacctgcttt accagcgcgc ctacggtttg cgaacagtaa atttgcgtct
     3961 gacgaacact tacggtccgc gccagcagat gtatcataac cggcagggct ttatcgcgtg
     4021 gtttatacgg caggcgatgg acggcgaaac catcgagctt ttcggcgacg gcaggcagag
     4081 ccgcgatttg aatcacgtcg atgacgtggt cgacgcgctg ctgc
//
LOCUS       DASWNE010000611         2851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_231651, whole genome shotgun sequence.
ACCESSION   DASWNE010000611 DASWNE010000000
VERSION     DASWNE010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231651"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VGB68_11990"
     CDS             <1..244
                     /locus_tag="VGB68_11990"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY0050004.1"
                     /translation="FLTLLGIWKILGAAAVLIPKFPRLKEWAYAGFFFAMSGAAFSHI
                     ALGDSTGEIFPSLLLLLLTAVSWYFRPADRKTVSVG"
     gene            269..841
                     /locus_tag="VGB68_11995"
     CDS             269..841
                     /locus_tag="VGB68_11995"
                     /inference="COORDINATES: protein motif:HMM:NF025432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HEY0050005.1"
                     /translation="MKINSNKRELSPEQQEELLGALKARFEKNMNRHQGLEWAEVRAK
                     LEAAPEKLWSLNEMERTGGEPDIIGFDKKTGDFIFYDCSAESPKGRRSICYDRQALES
                     RKEPKPENNAVDMAAAMGIELLTEEQYRELQKLGNFDTKTSSWVKTPSDIRKLGGALF
                     CDRRYGNVFVYHNGAESYYAARAFRGELRV"
     gene            complement(877..2127)
                     /locus_tag="VGB68_12000"
     CDS             complement(877..2127)
                     /locus_tag="VGB68_12000"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0050006.1"
                     /translation="MDKKERIILLLLTVLNFTHILDFMIMMPLGNYLMPYFDISSQQF
                     SMLVAAYTFSAGASGFLAAFFVDGFDRKKVLLFAYAGFLIGTLCCAVSPAYEILLVSR
                     IVAGLFGGLIGAQVLSIVADIVPYERRAAAMGMIMAAFSAASVFGVPFGLYIANLFNW
                     HAPFFFVVVLGVLLAPFLIKFLPKMDAHLHVENRLKISPTQLIGDVFRNSSQLYALAL
                     TAFLMMGHFMIIPFINPFMEFNMGFSKTQTPIIYMVGGALTMITSPLIGRLADRHGKY
                     NLFIFLALAGIPLIALITNLPAIPFYFVLCVTGLWFIISAGRFIPAQAMVSNVVPTER
                     RGSFMSFNSSVQQLFVGLASVLAGFIVVKMPDNKIENYEITGYLSIAIILFSIFIATM
                     LNRKLKEKSSEPAVVKKEELAALG"
     gene            complement(2191..2769)
                     /locus_tag="VGB68_12005"
     CDS             complement(2191..2769)
                     /locus_tag="VGB68_12005"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY0050007.1"
                     /translation="MNEKIKLALIYGSNRENRFCDTVANWAINQIGKSDEFDLTTIDP
                     AKMNLPRQIGKESNADLSDFRKRVREAEAFMIVVPEYNHGYPSALKFLIDAEYEGWRA
                     KPVSFVSYGGISGGIRAVEQLRQVFCELHAMTVRDSVHLPNAWRQFDKDGNIVKPQVP
                     EKMFSDTVRQLKWWANALREARKSEPYMTATV"
BASE COUNT          792 a          695 c          669 g          695 t
ORIGIN      
        1 ttttctgaca ctactgggca tttggaaaat tttgggagcg gcggcagtgc ttattcctaa
       61 atttccgcga ctcaaggaat gggcttatgc aggctttttc tttgcgatgt cgggagcggc
      121 attttcgcat atcgcgttgg gcgattcgac gggtgaaata tttccctcgc tgctgcttct
      181 gctgctgact gcggtgtcgt ggtatttcag acctgcggat agaaaaaccg tttcagtcgg
      241 ttaatttcaa cggagataat ataaaagtat gaagattaac agcaataaaa gagagttgtc
      301 gccggaacaa caggaagagc tgctcggagc attaaaagcc cgttttgaga aaaatatgaa
      361 tcgccatcaa ggtcttgaat gggctgaagt ccgggcaaag ctggaagccg ctcccgaaaa
      421 actgtggtcg ctcaacgaaa tggaaagaac cggcggcgaa ccggatatta tcggttttga
      481 taaaaagacg ggcgacttca ttttttatga ctgttcggcg gaaagtccga aaggtcgcag
      541 aagtatttgt tatgaccgtc aagcgctgga gtcaaggaaa gaacctaaac cggaaaataa
      601 cgctgtcgat atggctgccg ctatgggcat cgagctttta acggaggaac aatatcgaga
      661 gttgcagaaa ctcggaaatt tcgatacgaa aacgtcgagc tgggtgaaaa cgccttccga
      721 tattagaaaa ctcggcggcg cactcttttg tgatcgtcgc tacggtaatg tcttcgtgta
      781 tcacaacggc gcggaatctt actatgcggc aagggcgttt cgcggagagc ttcgggtctg
      841 aattccgcat aaaccgccgg gttttctacc cgttttttat cctaaagccg ccagctcttc
      901 tttctttacg accgcaggtt ccgagctttt ttctttgagc ttccggttga gcattgtcgc
      961 aataaaaata ctgaacaaaa tgatggcaat gcttaaatag cctgtaattt cgtagttttc
     1021 gattttatta tcaggcattt tgacgacgat aaatccagcc agaaccgaag ccaatccgac
     1081 aaataattgt tggacggacg aattaaagct cataaagctg ccgcgtctct cggtcggcac
     1141 gacgttgcta accatcgcct gcgcgggaat aaatctgccc gccgagatga tgaaccacaa
     1201 acccgtgacg cacagcacga aataaaacgg aatcgcgggc agattagtta tcaaagcaat
     1261 gagcggaatg cccgccaaag ccagaaaaat aaacaaatta tatttcccgt ggcgatccgc
     1321 cagccgcccg atgagcggcg aagtaatcat cgtcaacgcg ccgccgacca tataaattat
     1381 cggcgtctgc gttttggaaa atcccatatt gaattccata aacgggttga tgaacgggat
     1441 aatcataaaa tgccccatca tcaaaaaagc cgtcagcgcg agcgcgtata attgactgga
     1501 gtttctgaaa acatcgccaa tcaattgcgt cgggctgatt ttcagacgat tttcaacgtg
     1561 caggtgcgcg tccattttcg gcagaaattt aatcaaaaac ggcgccagca aaacgcccaa
     1621 gacgacgacg aaaaagaacg gcgcgtgcca gttgaaaaga ttggcgatat ataaaccgaa
     1681 cggcacgccg aaaaccgagg ctgccgaaaa cgccgccata atcattccca tcgccgccgc
     1741 gcgtctttca tacggcacga tgtcggcgac gatggacaaa acctgcgcgc cgatcaatcc
     1801 gccgaaaagt ccggcgacga ttctcgaaac gagcaaaatc tcgtaagcgg gcgagacggc
     1861 gcagcataaa gtcccgatca aaaatcccgc gtaagcaaac aataaaacct ttttgcggtc
     1921 gaagccgtcg acaaaaaacg ccgccagaaa acccgaagcg cccgcgctga acgtgtatgc
     1981 tgcaacgagc atcgaaaact gctgcgagct gatgtcgaaa tacggcatca ggtaattgcc
     2041 gagcggcatc ataatcataa aatccaggat gtgcgtgaaa ttcagcaccg tcaacagaag
     2101 caaaattatt ctttcttttt tatccattta atttcaaaac gattcgtcgt taatattaat
     2161 tcttgcattt agatttatcg accgccaata tcaaaccgtc gcggtcatat aaggctcgga
     2221 ttttcgagcc tcgcgcaaag cgtttgccca ccacttaagc tggcgaacgg tgtcggaaaa
     2281 catcttttca ggcacttgcg gcttaactat atttccgtcc ttgtcgaatt gccgccacgc
     2341 gttcggcaaa tgaaccgaat ctctgacggt catcgcgtgg agctcgcaga aaacctgccg
     2401 gagctgttcg acggcgcgga ttccgccgga aattccgccg tacgaaacaa acgaaaccgg
     2461 tttggcgcgc cagccttcgt attccgcatc gatcaaaaat ttcagcgccg acggatagcc
     2521 gtgattatat tctggaacca ctatcataaa agcctcggct tccctgaccc gtttcctgaa
     2581 atcggataaa tccgcgttcg attccttccc gatttgccgc ggaagattca tcttcgccgg
     2641 atcaatcgtc gtcaagtcga attcatcgct tttgccgatt tgattaatcg cccaatttgc
     2701 caccgtatcg caaaaacgat tttcacgatt gctgccgtag attaaagcca atttgatttt
     2761 ttcattcata agttttcaat ttttagaaca tctctgttcg ataaatccgt gttttcgatt
     2821 cttcaaaatc ctcttgccgc tgaatagttt t
//
LOCUS       DASWNE010000612         6311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_268994, whole genome shotgun sequence.
ACCESSION   DASWNE010000612 DASWNE010000000
VERSION     DASWNE010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6311
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268994"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3071)
                     /locus_tag="VGB68_12010"
     CDS             complement(<1..3071)
                     /locus_tag="VGB68_12010"
                     /inference="COORDINATES: protein motif:HMM:NF039983.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6079 family protein"
                     /protein_id="HEY0050008.1"
                     /translation="MKRIQEKVKDLVEVRPYRGLQDFLSDPSETLSAYHFTDQTAEMM
                     AKWLDKVADVQPFNGAAKALAGYRGVGKSHFLATLGAIVSHAELRSRVTEPMVAASAQ
                     RLKRRRHPVAYVRRGTHATLLEELKDALAPAIEVEPASLGGSLEELLKIASDKAGDLP
                     FVIIIDTAHDRANRVVRDDGVLLGELAELAKNLNLFIAVALDDDIAGADGVNAAIARN
                     YAIDYLDQEHLYRIVDTHIFPKHRQTQRLVHEIYTYFRGLLPSFRWSEQRFVSLYPLH
                     PLILEIAPFVRLYAPDFALLGFASEAGTKLLGRPANSLVGLDEVFDRTEIALRKAKDL
                     REAFITYDQLSSEVISHVPVMQRLQAKLVLKALMLLSLDGDGTTASEISAAMLIYDEN
                     APQNSIKQVEDLLETFVSIFPEAVNLKQEADREIRYSLKVSSKDSLNVALNEASLGIS
                     PDVVPKILRRFAAERFPDWIVQAENEATNSTDCHVIWRGGSRRIRLTWNLNDGAARSP
                     ENAADFLDWQILVNQPQNAHAPVAAGNENSTVVWQPAPLEREEEETILRYSILLNDAS
                     LRESFGEQVRAAGHTHSRAIEKIWNRIFLEDGKILIDGFAHGFTEKARAAHSLGEMLS
                     EMLTPLFEARYPAHPIFADVLGMDDVSKLVSDLFSGARQTLPEIQQLAASFASPLGLV
                     ALRGNNLVLDTEENLSQQPFVREILSLLSASGGETVLLKDISRRLKSQPYGLVAEAQH
                     LILAALVAQRRIEFVTSEGDRINRRSLDLKIVWDDIVGIARPTTVLHSSVELTNWART
                     LTAVDTFKTIDDPEDREKISKALENWLLDWESVSVLERFEQMPEEILNTKIWRLATRA
                     QKTFGAVAETVKEVLQETVSLEDCLQRIADAFSDSEKEFLECTRDLMQLEDFINGAGK
                     REAVWHYLAVCETTQDETIEKLRAKLLEFIREMAIAPSEILNRELERMWLLFHEAFIE
                     HFAIKHDTVMKSHHLLEKYDEMMRSDEWWEFENLSSLSIFQKNHWQKAQKI"
     gene            3398..4561
                     /locus_tag="VGB68_12015"
     CDS             3398..4561
                     /locus_tag="VGB68_12015"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY0050009.1"
                     /translation="MWLSAAAALPASVSAQTGTQAQNKQDDGKKKKEKKQKDSNKQDS
                     TQQQQQSTNNPKAPLTPNEDPNMIGKRNINKGSDSFFGWLGGSQEKELALGRQLAVQI
                     EQQAKLVEDPVITEYVNRVGQNIVLHSDAKIPFTIKVIQGDEVNAFALPGGYFYVYTG
                     LLLAADNEAELAGVMAHEIAHVAARHAMENSGKGTLMNYGILAGIIFGGPIASTILQN
                     VGGIGSSLAFFKFSRGAEVEADKLGVQYLYAAGYDPTAMSTMFEKLASRNKKKPNSLA
                     KLFTTHPQSTDRRDNSLGLVSRFPEKQEYVISTSEFQRVKGRLMKMTNAKAQVVSDID
                     ENGDSKPTLKRRQPSSDDPNAPTDTTKPKDDDDKPQLKRRGEPEPQASPSPEK"
     gene            complement(4654..5682)
                     /locus_tag="VGB68_12020"
     CDS             complement(4654..5682)
                     /locus_tag="VGB68_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050010.1"
                     /translation="MNKAGRILSINPPYAIPGGEVFIECENFQVDASITYGCFFDGQP
                     ARLVGASPNRIIAIIPETFGTSEVEVYLESGGDRSEPVNLTVARKLADELHIVANPAV
                     DPRDDSIILTRSGARGQQLPVTLFRLESDNYLQEMSTAVMNPTGVAFDRDGQLFVTNR
                     ADGEVCRVQSDEELTVLASGLGVATGLAFDRQGAMYVGDRSGTIYKISGLGAAQPFAA
                     LEPSVSAYHLAFDGADNLYVTAPSLSSYDSILRVDKEGYEDEFYRGLGRPQGLAFDTE
                     GNLYVAGCISSRRGIVRISPDGRDAGIFVAGMNVIGLCFTRKGEMIVATNEAVFNLPL
                     GIYGTLLD"
     gene            5732..>6311
                     /locus_tag="VGB68_12025"
     CDS             5732..>6311
                     /locus_tag="VGB68_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050011.1"
                     /translation="MGSYDIISVSEVWSSISNIHGFTQGLKHNQLRRIERLSSRRVAP
                     EQIISPELARQMSEISFETGRQIGVLLNRKGQVEYVMVGNAKRIEMPDFGRARVSVER
                     FRGLRCVHTHLLGEKLTQDDLTDLALLRLDLMATIQVDEKTGLPNLVHAAHLVPTTAE
                     KIDDEAHTLPYEFLEPQIPSQLETNFIGLINSL"
BASE COUNT         1489 a         1741 c         1661 g         1420 t
ORIGIN      
        1 cagattttct gcgccttctg ccagtgattt ttttgaaaaa tcgaaagcga tgaaagattt
       61 tcgaattccc accattcgtc gctgcgcatc atctcgtcgt atttttcgag cagatgatgc
      121 gatttcatca cggtgtcgtg cttgatggcg aaatgctcga tgaaggcttc gtgaaacaaa
      181 agccacattc tttccagctc gcgattgaga atctcgctcg gcgcaatcgc catttcgcga
      241 ataaattcca gaagtttggc gcgcagtttt tcgatggttt cgtcctgcgt cgtttcgcag
      301 acggcgagat aatgccagac ggcttcgcgt ttgcccgcgc cgttgatgaa atcttcgagc
      361 tgcatcaaat cgcgtgtgca ttcgagaaat tccttttccg aatcggaaaa agcgtcggcg
      421 atgcgctgca aacaatcttc gagcgagacg gtttcctgca aaacctcttt gaccgtttcg
      481 gcgaccgcgc cgaaagtttt ttgcgcgcgc gtggcgagcc gccagatttt cgtattgaga
      541 atttcttcgg gcatctgctc gaaacgctcc agaacgctca cgctttccca atccaaaagc
      601 cagttttcca gcgccttgct aattttttcg cggtcttccg ggtcgtcgat ggttttaaag
      661 gtatcgaccg ccgtcaaggt tcgcgcccaa ttggtcagct cgacggaaga atgcagaacg
      721 gtcgtcgggc gcgcgatgcc gacaatatcg tcccagacga tttttaaatc cagcgagcgg
      781 cgattgattc tgtcgccttc ggaggtgacg aattcgatgc ggcgctgcgc cacgagcgcg
      841 gcgagaatca aatgctgcgc ctcggcgacc aacccgtacg gctggctctt taaacggcgc
      901 gaaatgtctt tgagcaaaac ggtttcgccg ccgctcgcgc ttaaaagcga taagatttcc
      961 cgcacgaaag gctgctgcga aaggttttct tccgtgtcga gaaccagatt atttccgcgc
     1021 agcgcgacca gcccgagcgg cgaggcaaag ctggcggcga gctgctgaat ttccggtaaa
     1081 gtctggcgcg cgccgctgaa aaggtcgctg accagctttg agacatcgtc cattccgaga
     1141 acgtctgcga aaatcggatg cgccggataa cgagcttcaa aaagcggcgt cagcatttcc
     1201 gagagcatct cgccgagcga atgcgccgcg cgcgcttttt cggtaaaccc gtgcgcgaag
     1261 ccgtcgatta atatttttcc gtcttcgaga aaaatgcgat tccagatttt ctcaatcgcg
     1321 cggctgtgcg tgtgcccggc ggcgcgcacc tgttcgccga acgattcgcg caggctcgcg
     1381 tcgttcagca gaatcgaata gcgcagaatc gtttcttctt cttctctttc gagcggcgcg
     1441 ggctgccaga cgactgtcga gttttcgttt cccgccgcga ccggagcgtg ggcgttctgc
     1501 ggttgattaa ccagaatttg ccaatcgaga aaatcggcgg cgttttcggg actgcgcgcc
     1561 gcgccgtcat tcaaattcca ggtcaatctg atgcgccggc tgccgccgcg ccagatgacg
     1621 tggcaatcgg tcgaattggt cgcttcattc tccgcctgca caatccagtc cggaaaacgc
     1681 tcggcggcga aacgccgcaa aattttcggc acgacgtcgg gcgaaattcc cagcgaagct
     1741 tcgttgagag cgacgtttaa actgtctttg ctgctgactt tcaggctgta gcgaatttcc
     1801 cggtccgctt cctgtttgag gttgacggct tcgggaaata tcgagacgaa agtttccagc
     1861 aaatcttcga cctgtttgat ggaattttgc ggcgcgtttt cgtcgtaaat cagcatcgcc
     1921 gcgctgattt cgctggcggt cgtgccgtcg ccgtccagcg acaaaagcat cagcgccttt
     1981 aaaaccagct ttgcctgaag acgctgcatt accggaacgt gcgagatgac ttccgagctg
     2041 agctggtcgt aagttataaa cgcctcgcgc aaatctttgg ctttccgcag cgcgatttcc
     2101 gtccggtcaa aaacttcgtc cagcccgacc agcgaatttg ccgggcgacc cagaagcttc
     2161 gtgcccgctt cggaagcaaa accgagcagc gcaaaatccg gcgcgtatag acggacgaac
     2221 ggagcgattt ccagaatcag aggatgcagc ggataaagcg aaacgaatcg ctgctcgctc
     2281 catcgaaagc tcggcagaag cccgcgaaaa tatgtgtaaa tttcgtgaac gagccgctgc
     2341 gtctggcggt gtttggggaa aatatgcgta tcgacgatgc ggtaaagatg ttcctggtcg
     2401 agataatcaa tcgcgtaatt gcgcgcgatg gcggcgttga cgccgtccgc cccggcgatg
     2461 tcgtcgtcga gcgcgacggc gataaataaa tttaaatttt tagccagctc cgccaactcg
     2521 cccagcaaaa cgccgtcgtc gcgcacgacg cggttggcgc ggtcgtgagc cgtgtcgatg
     2581 atgataacga aaggcaaatc gcccgccttg tcggacgcga tttttaataa ttcttcgagc
     2641 gacccgccta agcttgccgg ttcgacttcg attgccggcg ccagcgcgtc ctttaattct
     2701 tccagcagtg tggcgtgcgt tccgcgccgc acgtatgcga ccgggtggcg gcggcgtttc
     2761 agacgctgtg cgctggcggc aaccatcggt tcggtcacgc gcgagcgcag ctccgcatgc
     2821 gagacaatgg cgccgagcgt cgccagaaaa tgacttttgc cgacgccccg ataacctgcc
     2881 agggctttcg ccgcgccgtt aaacggctga acgtcggcga ctttatcgag ccatttcgcc
     2941 atcatttccg ccgtctggtc ggtaaaatga tatgccgaaa gcgtctccga cgggtcggaa
     3001 agaaagtcct gcaagcctcg atacgggcga acttccacta aatcttttac tttttcttga
     3061 atgcgtttca ttgacggtaa aaattcgttt aatcgctaca tcgggaaagg aaaaattttt
     3121 gtcggtattt ctgggaatgc ggaatgtttt cgcgccgaaa ctgacggcgc aaatctactt
     3181 taacagtttt ttccgcagga ttaaatactt ttcgttaagt tttgtaaatt tatttcggat
     3241 ttaccaacct ttttttagtt ttcgtgcatc ttaaaagcat tgctctataa gcgattttaa
     3301 agatacaatt cacttatcgg cttgaacgta atttcttgga ggcataatgc gtagagaaaa
     3361 gattcggaaa tttaccgcgt ttcttttgtt ttcagctatg tggttaagcg ccgcggcggc
     3421 gctgccggcg agcgtttccg cgcagaccgg cacacaggca cagaacaaac aggacgacgg
     3481 caaaaagaaa aaagagaaaa aacaaaaaga ttccaacaaa caggattcca cacagcagca
     3541 gcagcaatcg accaataatc caaaagcgcc gctgaccccg aacgaagacc cgaatatgat
     3601 cggcaagcgc aacatcaaca agggttcgga cagctttttc ggctggctcg gcggctcgca
     3661 ggaaaaagag ctcgctctcg gcagacagct ggcggtgcag attgaacaac aagccaagct
     3721 ggtcgaagac ccggtgatta ccgaatacgt caaccgcgtc ggtcaaaaca ttgtgcttca
     3781 ttcggacgcg aaaattccgt tcaccattaa agtcatacag ggcgacgaag tcaacgcatt
     3841 tgccttgccc ggcggatatt tttatgttta cacgggcttg cttttagccg ccgacaacga
     3901 agccgagctg gcgggcgtga tggcgcacga aatcgcgcac gtcgccgcgc gtcacgcgat
     3961 ggaaaactcc ggcaaaggaa cgcttatgaa ttacggaatt ctggcgggaa ttatcttcgg
     4021 cggaccgatc gcctcgacga ttctgcaaaa cgttggcggc atcggcagct cgctcgcgtt
     4081 tttcaaattc agccgcggcg cggaagtcga agccgataag ctcggcgtgc agtatcttta
     4141 cgccgccggt tacgacccga ccgcgatgtc cacaatgttt gaaaagctcg cctcgcgcaa
     4201 caagaaaaaa ccgaacagcc tggcgaagct tttcacgacg cacccgcaat cgaccgaccg
     4261 ccgcgacaac agcctgggtc tggtctcgcg tttccccgaa aaacaggaat acgttatcag
     4321 cacatcggaa tttcagcggg ttaaaggtcg cttgatgaaa atgaccaacg ccaaagcgca
     4381 ggttgtttcg gacatcgacg aaaacggcga ttccaagccg actttgaaac gtcgccagcc
     4441 gagctccgac gacccgaacg cgccgaccga caccacaaag ccgaaagacg acgacgacaa
     4501 accgcagctt aaacgccgcg gcgaaccgga accgcaggca agtccgagtc ctgaaaaata
     4561 aataaaggca aaagcaaaaa ggcaaaagtt aaggagattt tcatccttaa cttttgcctt
     4621 tttgctttac ctttacccgg attatctttc gccttagtcc aacagcgtcc cgtagattcc
     4681 gagcggcaga ttaaaaacgg cttcgttcgt cgccacaatc atctcgccct tgcgcgtaaa
     4741 acataaaccg ataacgttca ttccggcgac aaatattccg gcgtcgcgac cgtcgggcga
     4801 aattcgcacg attccgcgac gactgctgat acagcccgcc acatagagat tcccttccgt
     4861 gtcaaaagcc agcccctgcg gtctgcccag cccgcgataa aattcatctt cgtagccttc
     4921 cttatcgact ctcaaaatcg agtcgtagct ggaaagcgac ggtgcggtca catacaaatt
     4981 atccgcgccg tcaaaagcca gatgatacgc cgaaaccgac ggctccagcg cggcgaaggg
     5041 ctgcgcggcg cccaaaccgg aaattttata gatggttccg ctgcggtcgc cgacatacat
     5101 cgcgccctgc cgatcgaacg ccaatccggt ggcgacgccc aaacctgaag ccagaaccgt
     5161 caattcctcg tcgctctgca cgcggcagac ttcgccgtcg gcgcgattcg tgacgaaaag
     5221 ctgcccgtcg cggtcaaaag ccacaccggt cggattcatc acggcggtag acatttcctg
     5281 caaataatta tcggattcaa gccggaaaag cgtaacgggc aactgctgac cgcgagcgcc
     5341 ggaacgagtc agaattatcg aatcgtctcg cgggtcgacc gccggattcg cgacaatgtg
     5401 aagctcgtca gccagctttc tggcgaccgt taaatttacc ggctcgctgc ggtcgccgcc
     5461 gctttccaga tagacctcga cttcgctggt gccgaaagtt tcgggaatga tggcgataat
     5521 tcgattcggc gaagcaccga ccagtcttgc cggctgtccg tcaaaaaaac atccgtatgt
     5581 aatgctcgca tcgacctgaa aattttcgca ctcgataaaa acttcgccgc ccggaatcgc
     5641 gtaaggcgga ttgattgaga gtattctgcc tgctttattc ataaactata attttacctt
     5701 ttttctgcat ctaagcaatt tgccaatcaa aatgggcagt tatgatataa tttccgtatc
     5761 ggaggtttgg agcagtatca gtaatattca cggttttact caaggcttaa agcataatca
     5821 gctgcgacgc atcgaacgcc tttcatcgcg ccgcgtcgca cccgaacaga taatttcgcc
     5881 cgagctggcg cgtcagatgt ccgagatttc gtttgagacc ggacgacaaa tcggcgtttt
     5941 gctgaatcgc aaaggtcagg tcgaatacgt aatggtcggc aacgccaagc gcatcgagat
     6001 gccggatttc ggtcgagcgc gcgtttcggt cgagcgtttt cgcggtctgc gctgcgtcca
     6061 cacgcacctg ctcggcgaaa agctgacgca ggacgacttg accgacctcg ctctgctgcg
     6121 gctggatttg atggcgacga ttcaggtcga cgaaaaaacg ggtttgccga atctggttca
     6181 cgccgcgcat ctggttccga cgaccgccga aaaaatcgac gacgaagcgc acacgctgcc
     6241 gtatgaattt ttagagccgc agattccttc gcagctggaa acgaatttta tcgggcttat
     6301 caattcgctc g
//
LOCUS       DASWNE010000613         4533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_56791, whole genome shotgun sequence.
ACCESSION   DASWNE010000613 DASWNE010000000
VERSION     DASWNE010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56791"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2529
                     /locus_tag="VGB68_12030"
     CDS             <1..2529
                     /locus_tag="VGB68_12030"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEY0050012.1"
                     /translation="RLVDSRATNASQASQIIQNQPVISWMAYTIHGNESASFEAMMQV
                     VYQLAASNEQATLDVLKNTVVLIVTGENPDGHERFVTWYNSVAMGNADRAALEHREPW
                     TIAGRFNHFRFDLNRDNLAVTQVETKNMQKAFFEWNPQIAVDHHGQPSQYHFPPAALP
                     INPNLPQPATNKWLEVFGRANAAQFDAQKWDFYVRDIFDLFYVGYWDSFPALNGATGM
                     TYETDGGGFKGLRWTRDDGSIATLRSAIAKHYVASLTTLETAAKNRAARLQDFYDFRR
                     TGMSETGTEKLKRIVIDPTRDRLKAAEMIENLRRMKIEVSVADSSFNSAAAHNYETKN
                     SPAERRSFPSGSYVIDLNQPQKRLIKAILEQDTPQDAAFVKDNLERFRRNEMRGKGQA
                     KEDYGFYDITAWSLPLAFGIDAFSTEDAGAISSTPVTAEYLENAKRGSVSGRAQIAYL
                     VPYETDAAGAMAIRLLKEGFRVAVSTKPLNAAGRNWKRGAFVIRVTRNPEKVHEAIAR
                     AAREMGVNVIAVNTGFSEEGDNSVGGENVVSLQAPRIAMVADEAVDQTSYGSIWWTLD
                     RYGIEFTPLTINNIRSGAINRYNVLIMPDGAAGRYFSAFGSGGISGLKEWISKGGTLI
                     TVRGASVFAALKDVNLTSSRLVGSDDDEEKGKAADEDEPDKRKEAESQPEPTPGPRTR
                     NQTPTPETAPTPSSELKFDKADGIAPTLPPIASPSSNAGKVPEGVPGAIMRATVDRTT
                     YLTYGVEDENVPVLLSSGYFFRVSKEGTNAFVFESKPRRPLTISGFVWEGNTERLLKG
                     TAYIIDEPSGGGHVVLFADEPFFRGIFRTTTRPFFNAILFNGTF"
     gene            3150..3635
                     /locus_tag="VGB68_12035"
     CDS             3150..3635
                     /locus_tag="VGB68_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY0050013.1"
                     /translation="MFTIKANYSDTVEVNTNLENVREFFSDIKNFVELMPGVESIHTD
                     AKGVTHWTIRAEIPIVGAMMQKFAVELSENDEERIEWSPVAGETKNLLRYAADFLEKG
                     KNLTLVHFSQVVEMRRNSARELHLLAGLAGESIISNEMSKKIAEMIKIFVRKAKEKLE
                     K"
     gene            3641..>4533
                     /locus_tag="VGB68_12040"
     CDS             3641..>4533
                     /locus_tag="VGB68_12040"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HEY0050014.1"
                     /translation="MKKPLFILFFILSLSLSVFAQLTLPRESQRQEIAQTIGDTKISI
                     VYHRPSVKARAVWGCQTTDVVPKGGVTYPCLVPYGQVWRTGANENTTFEVSRDVTIEG
                     QPLPAGKYGLHTIPNRDEWIVIFSKDNDDWGSYTYDEKKDALRVKVKPQSADFQETMS
                     LDFGNTTATTAKVAIRWEKLTVPFTVNVGDVSGRTLSMIREAITNRKPDDFRPLNQGA
                     SYVLASRLNNSYEEAIGWLDASIKARETFGNLSTKARILAEMGKIKEAVETGEKAVAV
                     GKASTPPANTADFEKSLAEWKA"
BASE COUNT         1154 a         1174 c         1197 g         1008 t
ORIGIN      
        1 cgactagttg attcgcgtgc gacgaacgcg tcgcaggcaa gtcaaatcat tcaaaatcag
       61 ccggtgattt cctggatggc ttacacgatt cacggcaacg aatcggcttc gtttgaagcg
      121 atgatgcagg tcgtctacca gctcgccgcg tcgaacgagc aggcgacgct cgacgtttta
      181 aaaaatacgg tcgttttaat agttacgggc gaaaatcccg acggtcacga acgttttgtc
      241 acgtggtata actcggtcgc gatgggcaac gccgaccgcg ccgcgctcga acaccgcgag
      301 ccatggacaa tcgccggacg cttcaatcat tttcgtttcg atttgaaccg cgataattta
      361 gccgtcacgc aggtcgaaac gaaaaatatg cagaaggcgt tttttgaatg gaatccgcaa
      421 atcgcggtcg accatcacgg gcagccttcg caatatcatt ttccgcccgc cgctttgccg
      481 ataaatccga atttgccgca gccggcgacc aataaatggc tggaagtttt cggacgcgcc
      541 aacgccgcgc agtttgacgc gcagaaatgg gatttctacg tgcgcgacat tttcgatttg
      601 ttttacgtcg gttactggga ttcgtttccc gccttgaacg gcgcgaccgg aatgacttac
      661 gaaaccgacg gcggcggctt caagggcttg cgctggacgc gcgacgacgg ctcgattgcg
      721 accctccgct cggcgattgc caaacattac gtcgcttcat tgacgacgct cgagacggcg
      781 gcgaaaaatc gcgcggcgcg gcttcaggat ttttacgatt tccgccgcac gggaatgagc
      841 gaaaccggaa cggaaaagct caaacgcatc gtcatcgacc cgacccgcga ccgcttgaaa
      901 gcggcggaaa tgattgaaaa cctgcggcga atgaaaatcg aagtttcggt tgccgattcc
      961 tctttcaatt ccgccgccgc gcacaattac gaaacgaaaa actcgcccgc cgagcgccgc
     1021 agcttccctt ccggctctta cgtgattgat ttaaaccagc cgcagaaacg ccttataaaa
     1081 gcgattctcg aacaggacac gccgcaggac gcggcttttg tcaaagacaa tctggaacgt
     1141 tttcgccgca acgaaatgcg cggcaaaggt caggcgaagg aagattacgg tttttacgac
     1201 atcaccgcct ggagcttgcc gctggctttc ggcatagacg ctttctcgac ggaagatgcg
     1261 ggcgcgattt cctcgacgcc ggtcacagcc gaatatctgg aaaacgccaa acgcggcagc
     1321 gtctcgggac gcgctcaaat cgcttatctc gttccttacg agacggatgc ggcgggcgcg
     1381 atggcaatcc ggcttttaaa ggaaggcttt cgcgtggcgg tttcgaccaa gcctctgaac
     1441 gccgccggaa gaaactggaa acgcggcgct ttcgtcattc gcgtgacgcg caatcccgaa
     1501 aaagttcacg aagctattgc gcgcgcggcg cgtgagatgg gcgtgaacgt aatcgccgtc
     1561 aacacgggtt tttccgaaga aggcgacaac agcgtcggcg gcgaaaacgt ggtctcgctg
     1621 caagcgccgc gcattgcaat ggtcgccgac gaagcggtcg accagacgag ttacggctcg
     1681 atttggtgga cgctcgaccg ctacggaatc gaatttacgc cgctgacgat taacaacatc
     1741 cggagcggcg cgataaatcg ctacaacgtc ctgattatgc cggacggcgc ggcgggacgt
     1801 tatttcagcg ccttcggaag cggcggaatc tccggcttga aagaatggat ttccaaaggc
     1861 ggcacgttga ttacagtgcg cggcgcgtcg gttttcgccg ctctgaaaga cgttaattta
     1921 acgtcttcga ggctggtcgg aagcgacgac gacgaggaaa aaggcaaagc tgccgacgaa
     1981 gacgagccgg acaaacgcaa ggaagccgaa agccaaccgg agccgacgcc cggtccccgc
     2041 accagaaatc aaacgccgac gcccgaaacc gcgccgacgc cttcgtcgga attaaaattc
     2101 gacaaagccg acggcatcgc gccgacgctc ccgccgattg cctcgccttc gtcaaacgcc
     2161 ggaaaagttc ccgaaggcgt gcccggcgcg attatgcgcg cgaccgtcga caggacgact
     2221 tatctgactt acggcgtcga ggacgaaaac gtgcccgttc tgctttcgtc aggctatttt
     2281 ttccgcgtct cgaaagaagg cacgaacgcc ttcgttttcg agtcgaagcc gcgccgaccg
     2341 ctgacgattt ccggcttcgt ctgggaagga aacaccgagc ggcttttgaa aggcacggct
     2401 tacatcatcg acgagccgag cggcggcggt cacgtcgttt tattcgccga cgagcctttt
     2461 ttccggggca ttttccgcac gacgacgcgc ccgtttttta acgcgatttt gtttaacgga
     2521 acgttttaaa cgcggaattc aaaatgcaaa gtgcaaaatt taagaaatga atttcatttt
     2581 ccactccgca ttttgaattt tcgacgttcc gcttcgcgtg aaaaccaact cgctttgcgt
     2641 gatattaaat ggtcattgca ggatattaaa tgttcattgc aggatattaa atgctcattg
     2701 tttgatatta aatgttcatt gcaggatatt aaatggtcat tgcaggatat taaatgttca
     2761 ttgcgtaata ttaaatgttc attgcgtaat attaaatggt tattacgtaa tattaaatgt
     2821 tcattgcatg atattgaatg gtcattgcgt aatattaaat ggtcattgtg tgatattaaa
     2881 tggtcattgt ttgatattaa atgctcattg tgtgataacg cgtgcgaatt gtgtgatatt
     2941 gtcccggctt tgcgtgataa cgcttcggca tttcgtgata acctctcggc tttgtgtaat
     3001 attaaatcag cctcgcgtgg acaacgcttc cgccgcgcgt gacgataaat aggtttcgtg
     3061 taatgtggcg tcggctttcc gaaaacttgc gccggaacga cgaaattttg caaaataaac
     3121 gtatccttat ttgttaattt caaatattta tgtttaccat caaagccaac tacagcgata
     3181 ctgtcgaagt aaatacaaat ctggaaaacg tgcgcgagtt tttcagcgac attaaaaatt
     3241 ttgtcgaatt gatgcccggc gtcgaaagca ttcacaccga cgcgaaaggc gtcacgcact
     3301 ggacaatccg cgccgaaatc ccgattgtcg gcgcgatgat gcagaaattc gccgtcgagc
     3361 tgtcggaaaa cgatgaagaa cgcatcgaat ggtcgccggt cgcgggcgaa acgaaaaatc
     3421 ttctgcgcta cgcggcggat tttctggaaa aaggcaaaaa tttaacgctc gtccattttt
     3481 cgcaggtcgt cgagatgcgt cgaaattcgg cgcgcgagct gcatttgctc gccgggctcg
     3541 ccggagaaag cattatcagc aacgaaatga gcaaaaaaat cgccgagatg attaaaatct
     3601 tcgtgcgcaa ggcgaaagaa aaactggaga aataaaccat atgaagaaac cgctttttat
     3661 actatttttt atcttatcgt taagcctgag cgttttcgct caactgacgt tgccgcgcga
     3721 atcgcagcga caggaaatcg cgcagacaat cggcgacacg aaaatttcca tcgtctatca
     3781 tcgcccgagt gtcaaagcgc gcgcggtttg gggctgtcaa acaaccgatg tcgtacccaa
     3841 aggcggcgtg acttatcctt gtctggtgcc ttacggtcag gtctggcgaa cgggcgccaa
     3901 cgaaaacacg acttttgaag tcagccgcga cgtaacgatt gaaggacagc ctttgcccgc
     3961 cggaaaatac ggcttgcaca cgattccgaa ccgtgacgaa tggattgtaa ttttcagcaa
     4021 agacaacgac gattggggca gttatacata cgatgaaaag aaagacgctt tgcgcgtcaa
     4081 ggtaaaaccg caaagcgccg attttcagga aacgatgtcg ctcgatttcg gcaataccac
     4141 ggcgaccacc gcgaaagtcg ccatcagatg ggaaaagctc accgtgccgt ttaccgttaa
     4201 tgtcggcgac gtttcgggca gaacgctttc gatgattcgc gaagcgatta caaaccgcaa
     4261 accggatgat tttcgcccgc tcaatcaggg cgcgagctat gttctggctt ccaggctcaa
     4321 caatagttac gaagaagcaa tcggctggct cgacgcttcc atcaaagcgc gcgaaacctt
     4381 cggcaacctt tcaaccaaag cgcggattct ggcggaaatg ggcaaaatca aagaagccgt
     4441 cgaaaccggc gaaaaagccg tcgccgtcgg taaagcctcc acgccgcccg ctaataccgc
     4501 cgatttcgag aaatctctgg cggagtggaa agc
//
LOCUS       DASWNE010000614         2830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_20977, whole genome shotgun sequence.
ACCESSION   DASWNE010000614 DASWNE010000000
VERSION     DASWNE010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20977"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1776)
                     /locus_tag="VGB68_12045"
     CDS             complement(<1..1776)
                     /locus_tag="VGB68_12045"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0050015.1"
                     /translation="MIGSTIHQYKILEKLGAGGQGTVYKAQDTKLDRTVVIKVLPPEL
                     TARTANFKRFEREAQLCSQLDHPNVCTIFDFSEQHGIFYIAMQYIEGRNVRQLVAGRP
                     LEIKSALGICIQVCDALAYTHSRGIIHRDIKAGNVMVTETGQAKILDFGLAKLIYDES
                     DFGKGVDKTEITEVGIPYGTATYAAPEQAKGERADHRADIFSTGVLLYEMLTGIWAFQ
                     GKTVIDVRHEVLYGTPKPLAEMRPEPIPPQLQLIVNKALAKNPKDRYQKISDMRDDLR
                     NIMQEISGVPMFQGDTFAPRHLGNNNVMKRAWGWITGKSVESPSQRSSSVSYQPNSST
                     EAPSLTATGSEKKSVAILPFKNLSQDVASQFYEFSLADAVITELAQLKSLIVRPSSVI
                     AKYQGTDTDPRDAGRELRVHAVLSAGFMRSGNKMRVTAQLLDVLTGDILWSDRIDAES
                     DDILALQDMIGLRILEGLELELSPKELARLGRRPTENAEAYEEFLRGRDNFGRFIFRT
                     VSMEDCEAAIANFRRAIELDPHFALAYSGLGACYANRVFKGMGDPEDYTYAESAFSKA
                     FMYDPNVTEARVLMVLIYLSRGEKKK"
     gene            complement(2049..2270)
                     /locus_tag="VGB68_12050"
     CDS             complement(2049..2270)
                     /locus_tag="VGB68_12050"
                     /inference="COORDINATES: protein motif:HMM:NF041212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HEY0050016.1"
                     /translation="MALVMYVKPNCPYCQKARDYYNENNIEFVEYDAQTDLARQKEML
                     DYSGGDLVVPCIVMDGEYVQSGWGDPPRG"
BASE COUNT          669 a          736 c          728 g          697 t
ORIGIN      
        1 ctttttcttt tcgccgcgcg aaagataaat cagaaccatc agaacgcggg cttccgtcac
       61 gttcgggtcg tacataaacg ccttgctgaa agccgattcg gcgtaagtgt aatcttccgg
      121 gtcgcccatt cccttgaaaa cgcggttggc gtagcacgcg cccaacccgc tgtaggcgag
      181 cgcaaaatgc gggtcgagct cgatggcgcg gcggaaattg gcgattgccg cttcgcagtc
      241 ttccatcgaa acggtgcgga aaataaagcg tccgaaattg tcgcgcccgc gtaagaattc
      301 ctcgtaagct tcggcgttct cggtcggtcg gcgaccgaga cgcgccaatt ctttcggcga
      361 aagctcaagc tccaagcctt ccagaatgcg cagtccaatc atatcctgaa gcgcgagaat
      421 atcgtcgctt tcggcgtcga tgcggtcgct ccagagaatg tcgccggtca gaacgtcgag
      481 caattgcgcc gtcacgcgca tcttgtttcc ggagcgcata aagcccgccg acaaaacggc
      541 gtgaacgcgc agctcgcgtc cggcatcgcg cgggtcggtg tccgttccct gatatttggc
      601 aatcaccgaa gacgggcgca caatcagcga tttcagctgc gccagctcgg taataacggc
      661 gtcagccagc gaaaattcgt aaaactgcga cgccacatcc tgactcagat ttttgaacgg
      721 caaaatggcg acgcttttct tctccgagcc ggtcgccgtc agcgaaggcg cttcggtcga
      781 gctgttcggc tgataggaga ccgacgacga tctctgcgac ggagattcca cgctttttcc
      841 ggtaatccag ccccacgcgc gtttcataac gttgttgttg ccgagatggc gcggcgcgaa
      901 cgtatcgccc tgaaacatcg gcacgccgga aatttcctgc attatgttgc gcaaatcgtc
      961 gcgcatatcg gagattttct gataacggtc tttcggattt ttcgccagcg ctttattgac
     1021 aatcagctga agctgcggcg gaatcggttc ggggcgcatt tcggcgagcg gtttcggcgt
     1081 tccgtagaga acttcgtggc gcacgtcaat cacggttttg ccttgaaaag cccagattcc
     1141 ggtcagcatc tcgtaaagca gaacgccggt tgaaaaaata tcggcgcggt ggtcggcgcg
     1201 ctcgcctttc gcctgttcgg gcgcggcgta agtcgccgtt ccatacggaa ttccgacttc
     1261 ggttatctcg gttttatcga cgcctttgcc gaaatcgctt tcgtcgtaaa tcagcttggc
     1321 aagcccgaaa tcgagaatct tcgcctgtcc ggtttcggtc accatcacgt tgcccgcctt
     1381 gatgtcgcgg tgaatgatgc cgcgcgaatg cgtgtaggcg agcgcgtcgc aaacctgtat
     1441 gcagatgccc aacgcgcttt ttatttcgag cgggcgaccg gcgaccagct ggcggacgtt
     1501 tctgccctcg atatactgca tcgcgatata aaaaattccg tgttgttcgc tgaaatcgaa
     1561 aatcgtgcag acattcggat ggtcgagctg cgagcagagc tgggcttcgc gctcaaagcg
     1621 tttaaagttg gcagtgcgag cggtcagctc cggcgggaga actttgatga cgaccgtgcg
     1681 gtcaagcttg gtatcctgcg ctttatagac ggtgccctgt ccgccggcgc caagtttttc
     1741 cagtattttg tattggtgaa tggtcgagcc aatcatttgg ttttcattta gatttacgat
     1801 ttgcgatttt ggttgaccgt aagctattaa gtttatgaaa aatttctaaa atggcaagtc
     1861 ggaaactaaa ctgttattaa gaaagtgatt gtaacaccga aagaaaaatg aaaccacaag
     1921 cagcgaccga aaaaagccgg agaaactcaa aatctctctc tcccgcgctt aattgtcctg
     1981 acctgtggct tcccgtctca aaatttttta ttcgccggca aactgccggc tgcttaacgc
     2041 gaaatcgttc agccgcgcgg cgggtcgccc cagcccgatt gaacgtattc gccgtccatt
     2101 acgatgcacg gcacgaccaa atcgccgccc gaatagtcga gcatttcctt ttgccgcgcc
     2161 aaatcggtct gcgcgtcgta ttcgacaaac tcgatgttgt tttcattata ataatcgcgc
     2221 gctttctggc aatacgggca attcggcttt acatacatta caagtgccat tttgctaaaa
     2281 acctccttgt tgtgtgaaca ttttagcaat taaagacgaa ttgataaaag ccgcccgcca
     2341 aaattttcaa tccgcgagcc gacggcgcgc ttcttcccgg caagcgcgcc gacagtaaat
     2401 ttcttcgaaa acatttgctc tgcggtcaaa ttctgtttta aagttttctc gataacgtaa
     2461 tcgagcgtgt aaaaaatctt ataaaataag ccgtccgtaa aacatatatg agaacgcctt
     2521 cggaaaaatt gctttctaaa agccgtcggt aaatactcgc cgagagcata gaaagattgc
     2581 gagcggcttt tagaaagccc gtcaagcgag ccgcgaattt gacgcgtccg attttcgtat
     2641 agaaatttat aacaaaaaat gaatatcgaa gattatcagg acatttttga gcctttcgac
     2701 aaattaatcg aaatcgagat tttggataaa accttcggcg tgcccgaaaa caacaatctg
     2761 ctgcgctgtt ttcaatatct ggcgctggaa gcgatttcga tgggcgaatt ttgctggaac
     2821 ggcgagtgcg
//
LOCUS       DASWNE010000615         1727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_513148, whole genome shotgun sequence.
ACCESSION   DASWNE010000615 DASWNE010000000
VERSION     DASWNE010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513148"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..1020)
                     /locus_tag="VGB68_12055"
     CDS             complement(208..1020)
                     /locus_tag="VGB68_12055"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY0050017.1"
                     /translation="MKQLSTPQRPQLVKHHQTGDMNFCEVLYPPQLRQARHTHKFASF
                     SYVLSGSYAESLERRTHRRLPSTVVFHPPAESHAVEFENDVRILSVEFSFEKLSDISR
                     QSVIFEASANCRSEKTNWLGERIYQEFQRMDAFSMLAIEGLILELLAEASRARVGADE
                     KGSPQWLRQSKDFLHANFTESFTLEEIARVADVHPVHLSRVFREKFGCTIGEYVRRLR
                     VEFASRQILSTEESLGGIAHAAGFSDQSHFNKTFKTAFGLTPAEYRKTFRRS"
     gene            complement(1270..>1727)
                     /locus_tag="VGB68_12060"
     CDS             complement(1270..>1727)
                     /locus_tag="VGB68_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050018.1"
                     /translation="GVSQMFLTHRDDVADHNAFVEKFAAARIMHAADGAKSLGVERVI
                     EDEEAVKLDDELTAIPVPGHTRGSQALLYKNKFLFTGDHLAWSPRRKSLTAFRSVAWY
                     SWEKQTRSMQKLLEYDFEWVLPGHGHIAQRSKQEMREHLTDCIEWMKRA"
BASE COUNT          432 a          426 c          450 g          419 t
ORIGIN      
        1 tggttgacgg cgacgcgccc gattagaagc ggcgcatttg tctgcgcgaa aacctgaagt
       61 ccgaaaacct gaagtccgaa aacaaacaga aacaaagcgg aaagtttgat tttaagcata
      121 agagaatctc ccaaaacatt atgttgacgc ttttatagca cgcggttgag ggcgcgtctt
      181 gaacagaaca aacattcttg tatcaatcta acttcggcga aaagttttcc ggtattcggc
      241 gggcgtcaga ccgaaagcgg tcttgaaagt tttattaaaa tggctctggt cggaaaaacc
      301 agccgcgtga gcaatcccgc ccagagattc ttcggtggaa agaatctgcc gcgacgcaaa
      361 ttcgacgcgc aggcggcgaa cgtattcgcc gatcgtgcag ccgaattttt cgcgaaacac
      421 gcgcgaaagg tgaaccggat gaacgtccgc cacgcgggca atctcttcga gcgtgaaaga
      481 ttcggtaaag ttggcgtgca ggaaatcttt cgactgcctg agccactgcg gagagccttt
      541 ttcatccgcc ccgacgcggg cgcgcgaggc ttccgccagc aattctaaaa tcaatccttc
      601 gatggcgagc atcgagaagg cgtccatccg ctgaaattcc tgataaatgc gctcgccgag
      661 ccagttcgtt ttctccgagc ggcagtttgc cgaagcctcg aatattacgg attgacggct
      721 gatgtcggaa agcttttcaa aactgaattc gacgcttaaa atccgcacgt cgttttcaaa
      781 ctcgacggcg tgcgattccg ccggcgggtg aaaaacgacg gttgagggca gtcgtcgatg
      841 cgtgcggcgc tccagacttt cggcgtaact gccgctgaga acgtagctga aagaagcgaa
      901 tttgtgcgtg tggcgcgcct gccgcaattg cggcggatag agcacttcgc aaaaattcat
      961 atcgcccgtt tgatgatgtt tgaccaattg cgggcgctga ggcgtcgata attgtttcat
     1021 aaaacacaaa atcctatgaa taaaattaat ggttaattta gagcgtttta atagatacgt
     1081 ctttcctaac gaacattatg aatcaaatgt tgcaggaaat gaattaaaat gtcgcagcaa
     1141 ataaaaagta acgctaaaag tgattgagtg ttcaggtgat tgagtgttca ggtgattaag
     1201 tgttcggtca actgtaattt cttcttcgcc aaacacctaa tagcctattc gcttattccc
     1261 ttagttctct caagctcgtt tcatccattc gatgcaatcc gttaaatgct ctctcatttc
     1321 ctgtttcgag cgctgcgcga tatgcccgtg tccgggcaga acccattcga aatcatattc
     1381 cagcagcttt tgcatcgagc gcgtctgttt ttcccacgaa taccacgcga ccgagcgaaa
     1441 agcggttaag cttttccggc gcggcgacca ggcgagatgg tcgcccgtaa acaaaaactt
     1501 gtttttgtaa agcaaagcct gcgagccgcg cgtgtgtccg ggaacgggga tggcggtcaa
     1561 ttcgtcgtcc agtttgaccg cttcttcgtc ttcgatgacg cgctcgacgc cgaggctttt
     1621 ggctccgtcg gcggcgtgca taatccgcgc ggcggcaaat ttttcgacaa aagcattgtg
     1681 gtctgcaacg tcgtcgcggt gcgtcagaaa catctggctg acgccgc
//
LOCUS       DASWNE010000616         1887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_269087, whole genome shotgun sequence.
ACCESSION   DASWNE010000616 DASWNE010000000
VERSION     DASWNE010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269087"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..676
                     /gene="msrB"
                     /locus_tag="VGB68_12065"
     CDS             203..676
                     /gene="msrB"
                     /locus_tag="VGB68_12065"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEY0050019.1"
                     /translation="MTPETQTTGKEMSESGYDITPISAETRERLAQDLTEEERHILLD
                     HGTEPPFCGNLTDNEQDGTYACKLCGLPLFRSDSKFHSSSGWASFYQSFDKDHIRYIR
                     DTAYGMIRTEIRCARCDGHQGHVFPDGPPPTGQRYCLNSASLEFFPAGEDVPQKG"
     gene            763..1827
                     /locus_tag="VGB68_12070"
     CDS             763..1827
                     /locus_tag="VGB68_12070"
                     /inference="COORDINATES: protein motif:HMM:NF005495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0050020.1"
                     /translation="MKITLKPIGVQVAVVFGASSGIGRLAALEFARRGAKVCVAARGE
                     AGLKSLVAEIEAAGGEAFYVVADAAVFEQVKDVAEKTVRRYGRIDTWIHSAAVFLFAT
                     VEKTDPEEYKHLIEINLLGQIYGAKAALPHLKEKGGALIHISSVEAYRAAPYQSAYGA
                     SKHALAGFLKVLRVELEHDRIPVAVTQILPAAINTPIYRTGRNKMLFKPRPVPPIYHP
                     QIVVDAILYAAENPVRDLVAGGAGLGVVYAERISPRLADWASHVIGYAGQDSGEASDG
                     NFAGNLFEPITGKDRVEGEFSGEQLKSDPYTWLQTNPKAKNGLLLAAGGIFGGILAWR
                     MLKDRNPRAAKGEEHAKLKM"
BASE COUNT          465 a          496 c          527 g          399 t
ORIGIN      
        1 cgaaaacggc aaaatcattt ccggcgtgcc cgctttcgat aaccgcgact ggctgcgctc
       61 gattgccgct ttcaggcgcc tgggcgagat ggcgcggacg cttcgtgatt tggaaaaaaa
      121 ggttaaggag cttgtcgaaa aacaaaacga atctggaact ttatgattga ttttgtcatc
      181 ataaagataa ggagaaaatt ttatgacacc ggaaacgcaa acgaccggaa aggaaatgtc
      241 cgaatcgggc tacgacatca cgccgataag cgccgagacg cgcgagcgcc tggcgcagga
      301 tttaaccgaa gaagaacgac acatcctgct cgaccacggc acggaaccgc cgttttgcgg
      361 caatctgacc gacaacgagc aggacggaac ttacgcctgt aaattgtgcg gcttgccgct
      421 tttccgctcg gattccaagt ttcattcgag ttcgggctgg gcttcgtttt atcaatcttt
      481 tgacaaagac cacatccggt atattcgcga cacggcttac ggaatgattc gcacggaaat
      541 tcgttgcgcg cggtgtgacg gtcatcaggg acacgttttt cccgatggtc cgccgccgac
      601 cgggcagcga tattgtctga actcggcttc gctggaattt tttccggcgg gcgaagacgt
      661 gcctcaaaaa ggctaaaggc taaaggcgaa gggcgaaagt aaaacaaaga ttttatcctt
      721 tatcctttag cctttagcct ttcgaataag ggaaggtgta aattgaaaat cactctaaaa
      781 ccaatcgggg ttcaggtcgc cgtcgtcttt ggcgcatcga gcggcatcgg caggctggcg
      841 gctttggaat ttgcccggcg cggcgcaaaa gtctgcgttg cggcgcgcgg cgaagcgggc
      901 ttaaaatcgc tggtcgcgga aatcgaagcg gcaggcggcg aggcgtttta cgtcgtcgcc
      961 gacgcggcgg ttttcgagca ggttaaagac gtcgccgaga aaaccgtccg gagatacggc
     1021 agaatcgaca cgtggattca ctccgccgcg gtttttcttt tcgccaccgt cgaaaaaacc
     1081 gacccggaag aatacaagca cttaatcgaa atcaatctac tgggtcaaat ctacggcgcg
     1141 aaagccgctc tgccgcactt gaaggaaaaa ggcggcgcgc tgattcatat ttcttccgtc
     1201 gaagcgtatc gcgccgcgcc ttatcaatcc gcttacggcg cttcaaaaca cgcattagcc
     1261 ggttttttga aagttttgcg cgtcgagctg gaacacgacc ggattccggt cgccgtcacg
     1321 cagattctgc ccgccgcgat aaacacgccg atttaccgaa cggggcgcaa caaaatgctt
     1381 ttcaagccgc gtcccgtgcc gccgatttat catccgcaaa tcgtggttga cgcgattctc
     1441 tacgccgccg aaaatccggt gcgcgatctg gtcgccggcg gcgccggtct gggcgtggtt
     1501 tacgccgaaa gaatctcgcc gcgtctcgcc gattgggctt cgcacgtcat cggttatgcc
     1561 ggacaggatt ccggcgaagc ctccgacgga aatttcgcgg gcaatctgtt tgagccgatt
     1621 accggaaaag accgggtcga aggcgagttt tccggcgagc agttaaaaag cgacccttac
     1681 acgtggctgc aaaccaatcc gaaagcgaaa aacggtcttc tgttagcggc gggcggaatc
     1741 ttcggcggaa tactggcgtg gcgaatgctc aaagacagaa acccgcgcgc cgccaaaggc
     1801 gaagaacacg caaaattgaa aatgtaaaat gatattcggc agcttccaaa tcacgtttga
     1861 aagctgccga atattatccg gaaattg
//
LOCUS       DASWNE010000617         2240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_21102, whole genome shotgun sequence.
ACCESSION   DASWNE010000617 DASWNE010000000
VERSION     DASWNE010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21102"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VGB68_12075"
     CDS             complement(<1..283)
                     /locus_tag="VGB68_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050021.1"
                     /translation="MLRKLLRPLALILALLFPTASPIMSQQLDPTFGNNGLVLTNFPP
                     PKSPLFSITRYAVESFPMNDGTILTLAQVNNQTPKGPPSIGMRLLKYSND"
     gene            446..1330
                     /gene="sucD"
                     /locus_tag="VGB68_12080"
     CDS             446..1330
                     /gene="sucD"
                     /locus_tag="VGB68_12080"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEY0050022.1"
                     /translation="MSVLVDKNTRLIVQGITGKEGTFHTQQMIEYGTNVVGGVTPGKG
                     GTAHENVPVFNTVDDAVRETGANASVIYVPPAFAADAIMEAADAGIPLVVCITEGIPV
                     ADMTKVRRFLEDKNTRLIGPNCPGVISPGKCKIGIMPGHIHKEGRIGVVSRSGTLTYE
                     AVGQLSALGLGQSTAIGIGGDPIIGTNHTDALRLFQADDETDAIVMIGEIGGTAEEDA
                     AAFARDNVTKPIVAFIAGQTAPPGRRMGHAGAIISGGKGTAAEKMKALTEAGIRVVES
                     PADIGKAIREVLGTSARG"
     gene            1358..1789
                     /locus_tag="VGB68_12085"
     CDS             1358..1789
                     /locus_tag="VGB68_12085"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEY0050023.1"
                     /translation="MAEEHENFLRRWFEEVWNKKNEGAIDRMCCADVVANGLTDAEGR
                     TICGVEAYKTLFRTFVSAYPDLKITVEDTVSEGDKIAARCRVTATHTGEGLGVAPTNQ
                     PLEFTGMTMVKIKDGKIAEAWNEFDFMKMYSQFGALKLNLQ"
     gene            1834..2040
                     /locus_tag="VGB68_12090"
     CDS             1834..2040
                     /locus_tag="VGB68_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050024.1"
                     /translation="MTENKQWYFYLWVIAGLLCAGNALQYLIGGQPYINSNLRNTLVI
                     IQLLLGVATIVYGYKAYGKRTVRK"
     gene            2037..>2240
                     /locus_tag="VGB68_12095"
     CDS             2037..>2240
                     /locus_tag="VGB68_12095"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEY0050025.1"
                     /translation="MKYSHTFRVRAGDIDEQGHVNNVAYVRWIQEIAVAHWFHATLPA
                     QQAKVVWVVLRHEVDYKRQSFENE"
BASE COUNT          572 a          542 c          630 g          496 t
ORIGIN      
        1 cgtcgttcga atacttgagc aatctcatcc caatcgaggg cggacctttc ggggtctggt
       61 tgttgacctg tgcaagagtc agaatagttc cgtcgttcat tgggaacgac tcgacggcgt
      121 atctggttat gctgaataac ggacttttgg gcggcggaaa attggtcaga accaagccgt
      181 tattgccgaa cgtcgggtcg agctgctgcg acataatcgg cgatgcggtc gggaacaaaa
      241 gtgccaaaat caaagcgagt gggcgaagga gcttgcggag cataaatttt tacctttcaa
      301 ataatcgtgg atagggaagt ttagcattga aaacgcttta cagcaccatt ttataaccga
      361 atcagaaagc cgaacgtgac aagccataga tttttcaata ttctatttgc ttgattatct
      421 tcaatttatt caaaggagta agaatttgag cgtcttagtt gataagaata cacgcctgat
      481 tgtgcagggc attacgggca aggaaggcac gtttcatacg cagcagatga ttgaatacgg
      541 cacgaacgtg gtcggcggcg tcacgcccgg caaaggcggc acggcgcacg aaaacgtgcc
      601 ggtctttaac acggtcgacg acgcggtcag ggaaaccggc gccaacgcct cggtcatcta
      661 cgttccgccg gcatttgcgg cggacgcgat tatggaagcg gcggacgcgg gaatcccgct
      721 cgtcgtctgc attaccgaag gcattccggt cgcggatatg acgaaagtca ggcgctttct
      781 ggaagacaaa aacacgcgtc tcatcggacc gaattgtccc ggcgtcatct cgcccggcaa
      841 atgcaaaatc gggattatgc ccggacatat tcataaagaa ggcagaatcg gcgtggtttc
      901 gcgttcggga acgctgacct atgaagcggt cgggcagttg agcgcgctgg gcttgggtca
      961 atcgaccgcc atcggcatcg gcggcgaccc gattatcggc accaatcaca ccgacgcttt
     1021 gcggcttttt caggctgacg acgagaccga cgccattgtg atgatcggcg aaatcggcgg
     1081 aaccgccgaa gaagacgccg ccgcctttgc ccgcgacaat gtgacgaaac cgattgtcgc
     1141 ctttatcgcc ggacaaaccg cgccgccggg acgccggatg ggacacgccg gagcgattat
     1201 ctcaggcggc aaaggcaccg ccgccgagaa aatgaaagcg ctgaccgaag ccggaatccg
     1261 cgtcgtcgaa tcgcccgccg acatcggcaa agctatccgg gaagttttag gaacgtcagc
     1321 ccgcggctag atttctacga atttctgacg ggaggatatg gctgaagaac acgaaaattt
     1381 tctgcgccgc tggtttgagg aagtctggaa caaaaaaaac gagggcgcga ttgaccggat
     1441 gtgctgcgca gatgtcgtcg ccaacgggtt gaccgatgcg gagggcagga cgatttgcgg
     1501 cgtcgaagct tacaaaactt tatttcgcac gttcgtttca gcttatcccg atttgaaaat
     1561 cacggtcgaa gatacggttt cggaaggcga taaaatcgcc gcccgctgcc gtgtgacggc
     1621 gacccatacg ggcgaaggat tgggcgttgc gccgactaac cagccgctcg aatttaccgg
     1681 aatgacaatg gtgaaaatta aggacggcaa aatcgccgag gcttggaacg aatttgattt
     1741 tatgaaaatg tattcgcagt tcggcgctct gaagctcaat ctgcaatgaa gttttcaaaa
     1801 tgcatttaaa gaattaactt cgggagtttg aatatgactg aaaataaaca gtggtatttt
     1861 tatctctggg ttatcgccgg attgctttgc gcgggaaatg cacttcaata tttaatcggc
     1921 ggtcagccct atatcaattc caatctcaga aatactctgg tgattataca attattatta
     1981 ggcgtggcga caatcgttta cggctataaa gcttatggca aaagaaccgt tcgtaaatga
     2041 agtattcgca tacgtttcgc gtccgagccg gcgatatcga cgagcagggg cacgtcaata
     2101 acgtcgctta cgtccgctgg attcaggaaa tcgccgtggc gcactggttt cacgcgacgc
     2161 tccccgcgca acaggcaaaa gtcgtctggg tcgtgctgcg tcacgaagtc gattacaagc
     2221 gccagtcttt tgaaaatgag
//
LOCUS       DASWNE010000618         1949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_513272, whole genome shotgun sequence.
ACCESSION   DASWNE010000618 DASWNE010000000
VERSION     DASWNE010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513272"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..895
                     /locus_tag="VGB68_12100"
     CDS             203..895
                     /locus_tag="VGB68_12100"
                     /inference="COORDINATES: protein motif:HMM:NF013260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0050026.1"
                     /translation="MRRLGGAIPRPGEVSLAHNGVLFLDELPEFDRSVLEVLRQPLED
                     KQVTISRAAMSLTFPALFTLVASMNPCPCGYFGSNRECRCSPPQIQRYVGKISGPLLD
                     RIDIHVDVPAVNFKELRGRGVPPGENSAAIRERIVKARQLQLRRFAGEKVFSNAAMSS
                     GQIRKYCHLSAESESLLERAMLRQGLSARAHDRILKVARTIADLEASENIEPTHIGEA
                     IGYRSLDRNYWS"
     gene            920..1354
                     /locus_tag="VGB68_12105"
     CDS             920..1354
                     /locus_tag="VGB68_12105"
                     /inference="COORDINATES: protein motif:HMM:NF026932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm50 family immunity protein"
                     /protein_id="HEY0050027.1"
                     /translation="MENKIENAEMLIDVFGRFPGFHDAEVWKITLNRKVIEKPSPTLE
                     ALIYVYSSGKQCLVNLEFTGVFGLKLENFNHQNALGDLEIREILEEDFKKYRTDARLF
                     GVTSKNEVKRLNFYVKFYYCFGVEAEFLCSSVEIASVEPVDY"
     gene            1678..>1949
                     /locus_tag="VGB68_12110"
     CDS             1678..>1949
                     /locus_tag="VGB68_12110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY0050028.1"
                     /translation="MAAFERIYERYHRRTYSLCLRMTNSATEAEDLTQEVFIQLFRKI
                     GSFRGESAFSTWLHRMTINQVLMHFRRRGVQNEKTTEDGEMPEQIV"
BASE COUNT          508 a          436 c          510 g          495 t
ORIGIN      
        1 atatacgtct attatttgcg aaagatttta cgccgccgca gtcggcgtga aagattttga
       61 gtgaatcgtt aaatttaaat tgagttcaac cttgtctcat ttacttttgc gatttcaaac
      121 cttgtctcat ttacttatag ctctagtctc acgacttttg gctaaaaagc agtgaaaaac
      181 aagctttcag tctcattttc ttttgcgccg actaggcggc gcgattccgc gtccgggcga
      241 agtcagtttg gcgcacaacg gcgttttgtt tctggacgag ctgccggaat tcgacagaag
      301 cgtgctggag gttttgcggc agccgctcga agacaaacag gtgaccatca gccgcgccgc
      361 gatgtcgctg acgtttccgg cgctttttac gctcgttgct tcgatgaatc cgtgtccgtg
      421 cggctatttc ggctcgaatc gcgaatgccg gtgcagcccg ccgcagattc agcgttacgt
      481 cggcaaaatt tcgggaccgc tgctggatag aatcgacatt cacgtcgacg ttccggcggt
      541 aaattttaag gagctgcgcg gcagaggcgt tccgccgggc gaaaactccg ccgcgattcg
      601 cgaaagaatc gtgaaagccc ggcagcttca gcttcggcgt ttcgcgggcg aaaaagtttt
      661 ctcgaacgcg gcgatgagct ccgggcaaat ccgaaagtat tgtcatctca gcgcggaaag
      721 cgaaagcctg ctcgaacgcg cgatgctccg gcagggtttg tcggcgcgcg ctcacgaccg
      781 gattttaaag gtcgcccgca cgattgccga tttggaagcg agcgaaaata tcgagccgac
      841 gcacatcggc gaagccatcg gctatcgctc gctggacaga aattactggt cgtgaaattt
      901 tagttttaat gcggaaataa tggaaaataa aattgaaaat gcggaaatgt taattgatgt
      961 tttcggcagg tttcctggtt ttcacgatgc cgaagtatgg aaaattactt tgaaccggaa
     1021 agtgatcgaa aagccttcgc cgacgctcga agcattgatt tatgtttatt cgtcaggcaa
     1081 acagtgtctg gtaaatttgg agtttacggg cgttttcgga ttaaagctgg aaaatttcaa
     1141 tcatcagaac gcgctcggcg atttggagat tcgtgaaatc ctggaagaag attttaaaaa
     1201 ataccggacg gacgcgaggc ttttcggcgt gacctcaaaa aatgaagtta aaagactgaa
     1261 tttttatgtc aagttttatt actgcttcgg cgtcgaggcg gaatttttgt gcagttccgt
     1321 cgaaatagcc tcagtcgagc cggtcgatta ttgaaataaa aagagccggc tgaaatttct
     1381 ttgccaaatc gaaaataaaa aagtaaaatc attttgcttt cgcatcaggc aactaatttt
     1441 tggaggttgc acatcataga cagggacgaa gtattacatt ccgaaaacga gcttttaggg
     1501 gcaaatatgg acttaaccgc agcggcagtt gtgaccggtg acgaagtgcc gtttccagtt
     1561 gaggaaaact gcaaaagcgt tcctgtaagt gtttctccgt ctgttaattc aacagagacc
     1621 gttatcaaag caaacgattc cgactttgaa ctcgcgcaga aagccgcatc aggaaatatg
     1681 gcggcgttcg aacgtattta cgagcgttat catcgacgga cttacagtct gtgcctgcgg
     1741 atgacgaaca gcgcgaccga agccgaagat ttgacgcagg aagtttttat ccagcttttt
     1801 cggaaaatcg gcagtttcag aggcgaatcg gctttttcga cctggctgca ccggatgacg
     1861 attaatcagg ttctgatgca ttttcgccgc cgcggcgtac agaacgagaa aaccaccgaa
     1921 gacggcgaaa tgccggaaca aatcgtcaa
//
LOCUS       DASWNE010000619         1814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_269281, whole genome shotgun sequence.
ACCESSION   DASWNE010000619 DASWNE010000000
VERSION     DASWNE010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269281"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VGB68_12115"
     CDS             <1..256
                     /locus_tag="VGB68_12115"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HEY0050029.1"
                     /translation="FIRLMKFQIERARRFYKKSENGVALLEKDARFSVLLALRIYAKI
                     LDEIERQNYDVFKRRAHTSFTQKIFSLPRIWRESRESAAN"
     gene            299..1426
                     /locus_tag="VGB68_12120"
     CDS             299..1426
                     /locus_tag="VGB68_12120"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEY0050030.1"
                     /translation="MKNILSLLLIVIFFFLPVTAQKRGAAEIKQLQSPQNPRLGFVIH
                     GGAGVIKRGSLSPEKEAEYRRKLEEAVLAGYKALQDGKPGLDAVEIAIRMLEDSPLFN
                     AGKGAVFTADGKNELDASIMDGKTLKAGAVAGLHQVKNPITLARAVMEKSPHVMMVGD
                     GAEKFAREQNIELVNEKYFWTQERWDSLQKIIRQEKEKEKSQKSKVKAQSLNSRPLDP
                     RPAMRASGFPNPDLRELAYNKFGTVGAVALDREGNLAAGTSTGGMTYKKFGRVGDAPI
                     IGAGTYANNSTCAVSATGWGEFFIRLGVARDISAMMEYRGLPIQQAADAVMNKVKTLG
                     GDGGVIAIDKFGNIGVSFNSEGMYRAYINSEGKPVIEIYND"
BASE COUNT          494 a          435 c          496 g          389 t
ORIGIN      
        1 ttttatccgg ctgatgaaat ttcaaatcga gcgggcgcgc cggttttata aaaaatcgga
       61 aaacggcgtc gcgcttctgg aaaaagacgc gcgtttttcg gttttgctcg ccctgcggat
      121 ttacgcgaaa attctggacg aaatcgagcg acagaattat gatgttttta agcgccgcgc
      181 gcatacgagt ttcacacaga agattttcag cttgccccga atctggcgcg aatcgcgaga
      241 atcggctgcg aattagaaaa aggaaagcaa aaataattgc tgccgaaacg gagtcaaaat
      301 gaaaaacatc ttatctttac tgttaatcgt catctttttc tttttgccgg ttacggcgca
      361 gaagcgcggc gcggcggaaa tcaaacaatt gcagtcgccg cagaatccgc ggctcggctt
      421 tgtcattcac ggcggcgcgg gcgtgattaa gcgcggctcg ctctcgccgg aaaaagaagc
      481 cgaatacaga cgcaagctcg aagaagccgt gcttgccggt tacaaggctt tgcaggacgg
      541 caaacccgga ctcgacgccg tcgaaatcgc gattcggatg ctggaagatt cgccgctttt
      601 caacgccgga aaaggcgcgg tctttaccgc cgacggcaaa aacgagctgg acgcatcgat
      661 tatggacggc aaaaccctga aagcgggcgc agttgccggt ctgcaccagg tcaaaaatcc
      721 gattacgctg gcgcgcgccg taatggaaaa atcgccgcac gtaatgatgg tcggcgacgg
      781 cgcggaaaaa tttgccagag agcagaatat cgagctcgtc aacgaaaaat atttctggac
      841 gcaggagcgc tgggacagcc tgcaaaaaat catcagacag gaaaaagaaa aagagaaaag
      901 ccaaaaatca aaggtcaaag ctcaaagttt aaattcccga cctctcgacc cgcgacccgc
      961 gatgcgcgcc tccggattcc cgaatcctga tttgcgcgag ctcgcctata acaaattcgg
     1021 cacggtcggc gcggtcgctc tcgaccgcga aggcaatctc gcggcgggaa catctacggg
     1081 cggaatgact tacaaaaaat tcgggcgtgt cggcgacgct ccgattatcg gcgcgggaac
     1141 ttatgcaaac aactcgacgt gcgccgtttc ggcgaccggc tggggcgagt tttttattcg
     1201 tctcggcgtg gcgcgcgaca tctcggcgat gatggaatat cgcggcttgc caatccagca
     1261 agccgccgac gcggtaatga ataaagtcaa aacgctgggc ggcgacggcg gcgtgattgc
     1321 gattgataaa ttcggcaaca tcggcgtttc gtttaattcc gagggaatgt atcgcgccta
     1381 tatcaacagc gaaggcaagc ccgtaatcga aatttataac gattaaaaag aatttccgga
     1441 caagaaaatt ttagccgcag agagaaagaa tgaaacagcg gacagaggcg gataaatgcg
     1501 gttaattaac cgaggatgac gggatgaaaa aaacaatgaa tgagaaattc ggaaggcgga
     1561 atttgcagtt ccgcattcat cgcttttctt atccgggctc gtcggcatca atctgcggtt
     1621 aattcttttt tgtgtttctc tcgtcgaata attcccgtgt ttctcgcatc gggcaataac
     1681 attcaatacc tcattgcttt ttgaaggctt ccggcagacg ggcgaaagcg aaaatggaga
     1741 atatcaaaag aaaattttta atgcgttacg gcgtggcgat tatatcggcg gcgtcgggcg
     1801 tgatgcttcg ctcg
//
LOCUS       DASWNE010000620         5534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_198152, whole genome shotgun sequence.
ACCESSION   DASWNE010000620 DASWNE010000000
VERSION     DASWNE010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5534
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198152"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VGB68_12125"
     CDS             complement(<1..731)
                     /locus_tag="VGB68_12125"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEY0050031.1"
                     /translation="MREIFFSADRLVAALLNLPKEKNLCLLDSCGVNHLDSHLLIAGF
                     NPFEILEINEPDADQSLTVLNEKLSPENTFAIFTIAYDFGLKLEKLKPRPKEFSGFGE
                     PDIFLALFECLIIYDYQTQKTFLCGDENKFDEIERTIRGAVDSVSTASSASSKISSNF
                     TEESYIAAIEQIKERIRRGDTYQTNLTQQIRAELPENLAPEQIFWRLRKNHPAPFAAF
                     LRRKNDTVISASPERFFSIAEGERQ"
     gene            827..2272
                     /locus_tag="VGB68_12130"
     CDS             827..2272
                     /locus_tag="VGB68_12130"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY0050032.1"
                     /translation="MTPNATEKITLIRGLGLVAAISIILGNVIGTGVFLKARVMTCNV
                     GDPTWVIIAWVAAGILSLAGALTYAELSAMKPQAGGEYVFLRDAYGRVWSFLYGWMLI
                     FVGKTGAQASVAVAFAIFLNNLLGGSLKQTLLQTQIFGYAYELTSLQIIAVMMIIIVT
                     TINCASVAVSGQLATMLTFVKIFLILLVGFGAFFLATGDFSHFSQIAEGGACEGVGDA
                     VKYGAPAYGFAAGFGAAMLGALWGYDGWNNLTLVAGEVQNPQRNIPIALIGGTVLIIF
                     LYVFVNIAYFYVLSPAEVAGISKDSSVAQEVAIRFLGSGALVIMTIGLMASSLGTLHT
                     SILTGARVPYAMGQDGVFFKKFGELSAGTRVPIIALIVQGIWACVLCLSGSFDTLTDY
                     VVFGSWIFYALATASVFVFRRTMPDAERPYKAFGYPIVPFLFLLAAGWLLINTMISTP
                     VQAFTGIILILLGLPVYYLLTKSNSQKREDI"
     gene            2281..2613
                     /locus_tag="VGB68_12135"
     CDS             2281..2613
                     /locus_tag="VGB68_12135"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY0050033.1"
                     /translation="MLKDRRSGRERRAIQRYNASIDIEWEGLVGRKTGTISDMSVVGC
                     FVLCSGEVEDSENVKIFFPLTDGRKIQLWGEVTNHVYEIGFAIRFIELSEAQKDFLEV
                     FVETLRED"
     gene            complement(2730..4223)
                     /locus_tag="VGB68_12140"
     CDS             complement(2730..4223)
                     /locus_tag="VGB68_12140"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEY0050034.1"
                     /translation="MTNPNVTIGAVAERLDAKFYGNAETFVSDVTHDSRQARAGALFV
                     AVRGLTMDGHRFIDDVMRRGAAGIISEFEKPESFEGVWLQVKDARVALAEAADVINGN
                     PSHHLKLIGITGTNGKTTTTYLTFALAEANGEKSAMLTTVEYRIGDESEPAVRTTPEA
                     SDTQRFLREALEKGCRMATMEASSQAIDLHRCDALRFKVAIFTNLTRDHLDYHHTMEN
                     YFDAKKKLFDGRLGEKPASCAINIDDRWGRKLAIELKVENQRVVTFAQDNPADLTAEN
                     VEVSLIKGTSFLLKTPRGERRVTSPLVGKPHVYNILSATAAALELGYDLDKIVEGIKI
                     CVGAPGRFERVEHEGNFAVVVDYAHTDDALLNTLKTARELTDGRIITVFGCGGDRDRS
                     KRVPMGEVAGRRSDLVIITSDNPRTEDPLKIISEIEKGVEKTGSPYLKISDRRDAIHL
                     AIHKAKANDVVIIAGKGHETYQIIGNDKFHFDDREAAKDALRKLQED"
     gene            4352..4621
                     /locus_tag="VGB68_12145"
     CDS             4352..4621
                     /locus_tag="VGB68_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050035.1"
                     /translation="MNWLKFILIVLGLIVGAWVIFALIGVVQVILWYAFILGALGIAG
                     YVGYQLFKPDKKPELKGRDAVSEIELDSAKSDRTLEEYRRKYLNK"
     gene            4863..>5534
                     /locus_tag="VGB68_12150"
     CDS             4863..>5534
                     /locus_tag="VGB68_12150"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEY0050036.1"
                     /translation="MNGEKNNYLFNTGLALAIALVMSSIVLGWFYKSKKDSDDAVTVT
                     GSARKRIKSDLVIWSAGVSYQAPKLADAYSRLSENVPRIKQYLIGKGINENQITISSI
                     SSTTMHGRDDNGNETSEITGYSLRQEVEVRSNDVERIGQIAREATELINQGILLESAP
                     PKYYYTQLGNLKIEMLGEAAKDAKTRAEQIAASTGNSIGTVRSARMGVMQITAADSTE
                     VSDSGI"
BASE COUNT         1235 a         1361 c         1509 g         1429 t
ORIGIN      
        1 ttttgccttt cgccttcggc aattgaaaaa aatctttcgg gcgaggcgga gatgaccgta
       61 tcgtttttgc gtcgcagaaa agcggcgaaa ggcgcgggat gattttttcg caggcgccag
      121 aaaatttgtt cgggcgcgag attttccggc agttcggcgc ggatttgctg cgtcagattc
      181 gtctgataag tgtcgccgcg ccggatgcgc tctttgattt gctcgattgc cgcaatgtag
      241 ctttcttcgg tgaaattgga cgagattttt gagctcgccg agctcgccgt gctgacggaa
      301 tcgaccgcgc cgcggattgt gcgctcgatt tcgtcgaatt tattttcatc gccgcaaaga
      361 aaagttttct gcgtctggta gtcgtaaatg attagacatt caaaaagtgc gagaaaaata
      421 tcaggctctc cgaaaccgga aaattctttc gggcgcggct tgagtttttc cagctttaag
      481 ccgaaatcgt aagcgatggt gaaaattgca aacgtatttt ccggcgaaag cttttcgttt
      541 aaaacggtga gagattgatc ggcgtccggc tcattgattt ctaaaatctc aaacggatta
      601 aacccggcaa ttaaaagatg agaatcgaga tgattgacgc cgcagctgtc cagaagacag
      661 agatttttct ctttcggcag attgagcaaa gcggcaacca gacggtcggc tgaaaagaaa
      721 atttcgcgca cggcagaaat tctaacacga aagatttatt tttatttgcg gctggattaa
      781 agtaagatgc aggcaagcat tttctttaga caaaaccgaa aaaactatga ctccaaatgc
      841 aaccgaaaaa ataactttga ttcgcggtct cggattggtg gcggcgattt cgattatttt
      901 aggaaacgtt atcggcacgg gcgtttttct gaaagcgcgc gtgatgacct gcaacgtcgg
      961 cgacccgacc tgggtgatta tcgcctgggt cgccgccggt attttatcgc tggcgggcgc
     1021 tctgacctac gccgagctgt cggcgatgaa gccgcaggcg ggcggcgaat acgttttttt
     1081 gcgcgacgca tacgggcgcg tctggagctt tctttacggc tggatgctga tttttgtcgg
     1141 caagacgggc gcgcaggctt cggtcgccgt cgcgttcgcg atttttctga acaatctgct
     1201 cggcggctcg ctcaaacaaa ctttgctgca aacgcagatt ttcggctacg cgtacgaatt
     1261 gacttcgctg caaatcatcg ccgtgatgat gattattatc gtcaccacca tcaactgcgc
     1321 ttcggtcgcc gtcagcgggc agctggcgac gatgctgact ttcgttaaaa tatttttgat
     1381 tttgctggtc ggcttcggcg cgttttttct cgccaccgga gatttttcgc acttttccca
     1441 aattgccgaa ggcggcgctt gcgaaggcgt cggcgacgcg gtgaaatacg gcgcgcccgc
     1501 ttacgggttt gccgccggat tcggcgcggc gatgctcggc gcgctttggg gatacgacgg
     1561 ctggaataat ttgactctgg tcgccggcga ggtgcagaat ccgcagcgca acattccgat
     1621 tgcgctgatc ggcggcacgg ttttgattat ctttttgtat gttttcgtca acatcgccta
     1681 tttctatgtt ctcagtccgg cggaagttgc gggcatttca aaagattctt cggtggcgca
     1741 ggaagtggca atcagattcc tgggctcggg agcgctcgtt attatgacta tcggcttgat
     1801 ggcttcgagc ctcggaacgc tgcacacttc tatcttaacg ggcgcgcgcg tgccttacgc
     1861 gatgggacag gacggcgtgt ttttcaaaaa attcggcgag ctttccgccg gaacgcgcgt
     1921 tccgattatc gctttaatcg tgcagggaat ctgggcttgt gttttatgtc tttcgggttc
     1981 tttcgatacc ttgacggatt acgtcgtttt cggctcgtgg attttctacg cgctggcgac
     2041 ggcttccgtt tttgtgtttc gccggacgat gcccgacgcc gaaagaccgt ataaggcgtt
     2101 cggctacccg attgtgccgt tcttgttttt gctggcggcg ggctggctgc ttataaatac
     2161 gatgattagc acgccggttc aggcttttac gggaattatc ttaattttgc tgggattacc
     2221 cgtttattac ttattgacga agagtaattc acaaaaaagg gaagatatct aagagtagtt
     2281 atgctaaaag accgtagaag cgggcgcgag cgccgcgcaa tacagcgcta taacgccagt
     2341 atcgacattg aatgggaagg tttggtcggc agaaaaaccg ggacgattag cgatatgagc
     2401 gtcgtcggct gttttgtttt gtgctcgggc gaagtcgaag acagcgaaaa cgtgaaaatc
     2461 ttttttccgc tcaccgacgg gcgcaaaatc cagctctggg gcgaagtcac caatcacgtc
     2521 tacgaaatcg gctttgccat tcgttttatc gagctttcgg aagcgcagaa agattttctc
     2581 gaagtgtttg tcgagacttt aagagaggat taaaacagga aataaaatac agccgcagaa
     2641 aacacggcga gcccggagaa atgatttttg ttttctcccg ggcgagcttc gcgttcttcg
     2701 gcggctgaat ttccggcggc ttttcgcgct taatcttcct gcaattttct cagggcgtct
     2761 ttcgccgctt cgcggtcgtc gaaatgaaat ttatcgttgc cgatgatttg ataagtttcg
     2821 tgacctttac cggcgataat cacgacgtcg tttgcctttg ctttgtgaat cgccaggtga
     2881 atcgcgtcgc ggcggtcgga gattttcaaa tagggcgagc cggttttctc gacgcccttt
     2941 tcgatttccg agatgatttt cagagggtct tccgtgcgcg ggttgtccga ggtgataatg
     3001 accaaatcgc tgcgtcgtcc ggcgacctcg cccatcggca cgcgtttgct ccggtcgcgg
     3061 tcgccgccgc agccgaaaac ggtgattatc cgcccgtcgg tcagctcgcg cgccgttttc
     3121 agagtgttca aaagcgcgtc gtccgtgtgc gcgtaatcga cgacgaccgc aaaatttccc
     3181 tcgtgctcga cgcgctcgaa gcgtccgggc gcgccgacgc agatttttat tccttcgacg
     3241 attttgtcca aatcgtagcc gagttcgagc gccgcggcgg tggcggaaag aatattgtaa
     3301 acgtgcggct tgccgaccag cggcgatgtg acgcgtcttt cgccccgcgg cgttttcaat
     3361 aaaaacgaag ttccttttat cagcgaaacc tcgacgtttt ccgccgtcaa atccgccgga
     3421 ttgtcctgcg cgaaggttac gacgcgctga ttttcgactt ttaattcaat ggctaatttt
     3481 ctgccccagc ggtcgtcgat atttatcgcg cacgacgcgg gtttttctcc cagacgaccg
     3541 tcgaaaagct tctttttcgc gtcgaaataa ttttccatcg tgtgatgata atccagatgg
     3601 tcgcgcgtca ggttggtaaa aatcgccact ttaaagcgca gggcgtcgca gcgatgaagg
     3661 tcaatcgcct gcgaagaagc ttccatggtc gccatccggc agcctttttc cagcgcctcg
     3721 cgcaaaaatc tctgcgtgtc cgaagcttcg ggcgtcgtcc tgacggctgg ctcggattcg
     3781 tcgccgatgc ggtattcgac cgtcgtcagc atcgcggatt tttcgccgtt ggcttcggct
     3841 aaagcgaacg tcagataagt ggtcgtcgtt tttccgttcg ttccggtgat gccgattagt
     3901 ttcagatgat gcgacggatt tccgtttatc acgtcggcgg cttccgccag agcgacgcgc
     3961 gcgtctttga cttgcagcca gacgccttcg aagctttccg gtttttcaaa ttcggagatg
     4021 atgcccgccg cgccgcgccg cattacatcg tcgataaaac gatgcccgtc catcgtcaaa
     4081 ccgcgcacgg cgacgaaaag cgcgcccgcg cgagcctgtc ttgagtcgtg cgttacgtcc
     4141 gagacaaacg tttcggcgtt tccgtaaaat tttgcatcca atctttcggc aaccgcgccg
     4201 attgttacat tcggattcgt caagaaaatt tcccctgtag ataaaaaatc gaagttggtt
     4261 ggattatacg acagacggga aaaatgcacg aaacgcgtct tgtaaagatt ttgaaaaaag
     4321 ctaaaataaa aaggaaaggt tttccgtaaa gatgaactgg ttaaaattta ttctcatcgt
     4381 tctcggtttg attgtcggcg cgtgggtcat cttcgcgctt atcggcgtcg ttcaggtaat
     4441 cttgtggtac gcgttcattt taggcgccct tggcattgcc ggttacgtcg gctatcaatt
     4501 attcaagccc gacaaaaagc ccgagcttaa aggtcgggac gccgtttcgg aaatcgagct
     4561 cgacagcgcc aaaagcgacc gcacgctgga agaatatcgc cgcaaatatt tgaacaagta
     4621 attacgaata atcacgaata aaaagaaatg accttcgagc tttgacccgc gccggaattc
     4681 gaccggcaaa ggtcatttct ttcttccgtt tgaccgcctc aaaatctctc aggcatcttc
     4741 taaaaatttc taataaatcc ctgataatcc gtgtggttat ccacagccgt tcgccgtgtc
     4801 ggtcaacgtt ttaacgcctt gccgactgtt ttatagtcga tgctgctttg aggaagcaaa
     4861 agatgaacgg agaaaaaaat aattatttgt ttaacaccgg actggctctg gcaatcgctt
     4921 tagtgatgtc gtccattgtg ctgggctggt tttataagag taaaaaagac agcgacgacg
     4981 cggtgacggt gacgggttcg gcgcgcaagc gcatcaaatc ggacttggtt atatggagcg
     5041 cgggcgtgtc gtatcaagcg cctaaattag ccgacgctta cagccggctt tcggaaaatg
     5101 tgccgcgcat caagcagtat ctgatcggca aaggcatcaa cgaaaatcag ataacgattt
     5161 cttccatctc gtcgaccact atgcacgggc gagatgataa cggaaatgaa acgtcggaga
     5221 ttaccggcta ctctctgcgc caggaagtcg aggtcaggtc aaacgacgtc gaacgcatcg
     5281 ggcagattgc gcgcgaggcg acggagttga ttaatcaggg catcctgctc gaaagcgcgc
     5341 cgccgaaata ttactacacg cagctcggca atttgaagat tgaaatgctc ggcgaagcgg
     5401 cgaaagacgc gaaaacgcgc gccgagcaaa tcgccgccag caccggaaac tcgatcggca
     5461 cggtgcgctc ggcgcggatg ggcgtgatgc agattacggc ggcggattcg accgaagttt
     5521 ccgattccgg catc
//
LOCUS       DASWNE010000621         1811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_21527, whole genome shotgun sequence.
ACCESSION   DASWNE010000621 DASWNE010000000
VERSION     DASWNE010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21527"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1291
                     /locus_tag="VGB68_12155"
     CDS             <1..1291
                     /locus_tag="VGB68_12155"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEY0050037.1"
                     /translation="ANAQAHDETRKFNFQLNEKVQELRALLDLVRGLTSTLEAAEVAR
                     LLILTLTGRWAVGKYALFAQKENHPPVIRQKGISLPSLEAFDEILKDLPEAVSVADLP
                     TGELKNTLETEKAQLVFPINPSASTRGVLVLGGRLGKLPFSAADLEFGAGLVAQAGVA
                     LENSWYVLETIERKKMEQELLLAASIQQGLFPEFLPKIEGYDIAARNRPALQCGGDYY
                     DVLPIERITGAGEKSYLLCVADVSGKGLPASLLMSNMQATLRALLGRVPTLAELAALT
                     NQLLYATTPSNKFVTAILFEIFPSSGKAAYVSAGHGDCVLLRNTGEIEKFDSTGLPLG
                     MMDSDMLEMLGKGYEEKHLELNAGDLLALYSDGVTEAYDIDENEWGDERLLKCLRPIL
                     ESSSQLIVGEVFNAIDDFAQTAPQHDDITLMIIKRLH"
BASE COUNT          467 a          438 c          449 g          457 t
ORIGIN      
        1 tgccaacgcg caggcgcacg acgaaaccag aaaattcaac tttcagctca acgaaaaagt
       61 tcaggaactg cgcgctctgc tcgatttggt gcgcggtctg acctcgacgc tcgaagccgc
      121 ggaagtcgcg cgtcttttga ttctgaccct gaccggacgc tgggcggtcg gcaaatacgc
      181 gctgttcgcg caaaaggaaa atcatccgcc ggtcatccgg caaaaaggca tcagtttgcc
      241 ttcgctcgaa gccttcgacg agattctaaa agatttgccc gaagccgtca gcgtcgccga
      301 tttacctacg ggcgagctga aaaacacgct ggaaaccgag aaggcacagc ttgtttttcc
      361 gattaatccg tcggcttcga ccaggggcgt tctcgtgctc ggcgggcgtc tcggcaaact
      421 gccgttttcc gcagccgatc tcgaattcgg cgccgggctg gttgcgcagg cgggcgtcgc
      481 gctcgaaaat tcgtggtatg ttctggaaac catcgaacgc aagaaaatgg aacaggaatt
      541 attgctcgcc gccagcattc agcagggact ttttcccgaa tttctgccga agatagaggg
      601 ttatgacatc gccgctcgga atcgtcccgc gctgcaatgc ggcggcgatt attacgacgt
      661 tctgccgatt gagagaatca cgggcgcggg cgaaaaatca tatctgttat gcgtcgccga
      721 cgtctccggc aaaggcttgc cggcttcgct tttaatgagc aatatgcagg cgaccttgcg
      781 cgcgctgctg ggtcgcgttc cgacgctcgc cgagctcgcc gctttgacca accaactgct
      841 ttacgcgacc actccgtcga acaaattcgt gaccgcgatt ttgttcgaaa ttttcccgtc
      901 gtccggcaaa gccgcttatg tcagcgccgg gcacggcgat tgtgtgcttt tgagaaatac
      961 gggcgaaatt gaaaaattcg attccaccgg cttgccgctc ggcatgatgg attccgatat
     1021 gcttgaaatg ctcggcaaag gctacgagga aaaacatctt gaattaaacg cgggcgacct
     1081 tctcgcgctt tattcggacg gcgtaacgga agcctacgac atcgacgaaa acgaatgggg
     1141 cgacgagcgg cttttaaaat gcctgcgtcc gattctcgaa agctcttcgc agcttatcgt
     1201 cggcgaagtt ttcaatgcga ttgacgattt cgcgcagacc gcgccgcagc acgacgatat
     1261 taccttgatg attatcaaac gactgcatta agctggcggc gccggcgttt gttttgaaaa
     1321 tatacggctg ttcgcggctt tgcacaaaaa taaagaaagg gaaaattgta aattgtaaat
     1381 tgtaaattct aaattaatgt tcggtatctt ttgtgaagta attgagaatt aataattgag
     1441 aattaataat ttagaattta caatcagcca tcggaatttt tctcgatttc ctttattttt
     1501 gtgcaaagca gatgttcgcc aatcttaaat ctgccggaat aaattggaga agcaaattgt
     1561 cataaactat attttaaaaa attcatctgt gattttaagt gtgccgggca aaactttaat
     1621 gactcgcctg aagctcctga ggatttcaac aaaatcaatt tgagtttgtt aatgaaattg
     1681 ttccagcaat tcccaataga agaatgaaat cgaacaactg cccgggaaaa attatctttt
     1741 tctctttttc agagtttgca taacttcgtc caatgaaact tcttttgcgg cgagcaaaac
     1801 cagcaaatga a
//
LOCUS       DASWNE010000622         1775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_232126, whole genome shotgun sequence.
ACCESSION   DASWNE010000622 DASWNE010000000
VERSION     DASWNE010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232126"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..>1775)
                     /gene="cphA"
                     /locus_tag="VGB68_12160"
     CDS             complement(14..>1775)
                     /gene="cphA"
                     /locus_tag="VGB68_12160"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: protein motif:HMM:TIGR02068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEY0050038.1"
                     /translation="YVIIERQLLGEDFRALVVNNQLVAVAERTPAHVVGDGESTIAQL
                     IDKVNHDPRRGYGHEKVLTQIVVDDQTRELLRAKDYTLATILNKGEKLILKTTANIST
                     GGTAIDRTDDVHPENVFLFERIAQIIGLDIAGVDVIAPDISTPLRENGGGIIEVNAAP
                     GFRMHLSPSDGIGRNVAEHVIDMLFPPGKPYRIPIFSITGTNGKTTVTRFIAHILKNS
                     GCTVGFTTTDGTYIGNNLIVSGDNTGPVSAQLVLKDPTVDVAVLETARGGIIRSGLGF
                     DHSDIGVVTNIASDHLGLKDVNTLEDLARVKSVVPRSVSKRGFAVLNAEDDHVFGMRK
                     LVEGSVALFSMDEHNERVQQHAEEGGVSCVYENGYITLLKGKWKVRVEKVTNIPITFG
                     GRAGFMIQNALAATLACFVHGVSIEDIRNGLSTFTAGTAQTPGRLNFIEIDNFTVLMD
                     YAHNPAGLTALIKFITKLPYKHRSVILNGTGDRRDDDIREMGRLCAENFEKIIIRRGN
                     YLRGRDEKDMYRLLEEGINADGNKPEVKIIPESDEAIRYALENARQGELIVTLGDRVT
                     HDIEMVKKFRDEMNPIAAID"
BASE COUNT          358 a          508 c          480 g          429 t
ORIGIN      
        1 ttttctctga cggttaatca atcgcggcaa tcgggttcat ctcgtcgcgg aatttcttga
       61 ccatttcgat gtcatgggtg acgcggtcgc cgagcgttac gataagctcg ccctgacgcg
      121 cattttccag cgcataacga atcgcctcgt cgctttcggg aatgatttta acttccggtt
      181 tgttgccgtc ggcattgatt ccttcttcca gcagtcgata catatccttt tcgtcgcgcc
      241 cgcgcaggta attgccgcgc cggataatga ttttttcgaa gttttcggcg cagagcctgc
      301 ccatttcgcg gatgtcgtcg tcgcggcggt cgcccgtgcc gttcagaata acgctgcgat
      361 gtttatacgg cagcttggtt atgaatttta tcagcgccgt caaacccgcc ggattgtgcg
      421 cgtaatccat caaaacagtg aaattgtcga tttcgataaa gttcagtcgt ccgggcgtct
      481 gcgccgttcc ggcagtaaag gtcgaaagcc cgttacgaat gtcttcgatg gaaacgccgt
      541 gcacaaaaca cgcgagcgtc gccgccagcg cgttctgtat cataaaaccg gcgcgcccgc
      601 cgaaagttat cggaatgttc gttacttttt cgacgcgaac cttccatttg cctttcaaaa
      661 gcgtgatgta tccgttttcg taaacgcagg aaacgccgcc ttcctcggcg tgctgctgaa
      721 cgcgctcgtt atgctcgtcc atcgaaaaca gcgcgacgct gccttcgact aattttcgca
      781 tcccgaaaac gtggtcgtcc tcggcattca aaacggcaaa gccgcgtttg gaaaccgagc
      841 gcggcacgac cgatttgacg cgcgccaaat cctccagcgt gttcacgtcc ttcaagccca
      901 gatggtcgga agcgatgttc gtcacgacgc cgatgtccga gtggtcaaaa cccaagcccg
      961 agcgaatgat tccgccgcgc gccgtctcca agacggcgac atcgaccgtc gggtctttca
     1021 gaacgagctg cgccgaaacg ggtcccgtgt tgtcgcccga aacaatcaga ttgttgccga
     1081 tataggttcc gtcggtcgtc gtaaagccga ccgtgcagcc gctgtttttc agaatatgcg
     1141 cgataaagcg tgtgacggtc gttttgccgt tggttccggt aatcgaaaaa atcgggatgc
     1201 gatacggttt cccgggcgga aaaagcatat caatcacgtg ttcggcgacg ttgcgcccga
     1261 tgccgtcgct cggcgaaagg tgcatccgga agccgggcgc ggcgttgacc tcgataattc
     1321 cgccgccgtt ttcgcgcagc ggcgtcgaaa tatccggcgc gataacgtcg acgcccgcga
     1381 tatccagccc gataatctgc gcgatgcgct caaataaaaa aacgttttcc ggatgaacat
     1441 cgtccgtgcg gtcgattgcc gtgccgccgg tcgaaatatt tgccgtcgtc tttaaaatca
     1501 gtttttcgcc cttgtttaaa atcgtcgcca gcgtgtaatc ttttgcccgc aaaagctctc
     1561 tggtctggtc gtcgacgacg atttgcgtca gaactttttc gtgcccgtag ccgcggcgcg
     1621 ggtcgtgatt gaccttatcg ataagctgcg cgatggtgct ttcgccgtcg ccgacgacgt
     1681 gcgccggtgt gcgctcggcg accgcgacca actgattatt gacgaccagc gcgcgaaaat
     1741 cttcgccgag cagctggcgc tcgataatca cgtat
//
LOCUS       DASWNE010000623         1560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_617702, whole genome shotgun sequence.
ACCESSION   DASWNE010000623 DASWNE010000000
VERSION     DASWNE010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617702"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VGB68_12165"
     CDS             complement(<1..519)
                     /locus_tag="VGB68_12165"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY0050039.1"
                     /translation="MIETIKTDVIIVGAGPTGLALACQLIRYGIDFVIFDRKEGITDL
                     SKAIAVQARTLEIYEQLDLAERAVEAGAIARTANIVADGEIRGSINLSDIGKDLSPFP
                     FALLLEQSKNERLLDDYLQRHGKNVRWKTALESISQAGDEVTATIKTADGETRTMAAK
                     YLVGCDGAKSLVR"
     gene            complement(711..1199)
                     /locus_tag="VGB68_12170"
     CDS             complement(711..1199)
                     /locus_tag="VGB68_12170"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY0050040.1"
                     /translation="MPKEHIYETNLVWTGNLGDGTKTYTGYSRAHEISIEGKPAIVGS
                     SDPHFRGDASRYNPEELLVAALSACHMLYYLHLCADAKIVVTDYTDSATGKMVETPDG
                     GGHFTEVVLRPRVRISKESDTEKAKEIHRRAHELCFIANSVNFPVRAEPDFMEPDIND
                     AG"
     gene            complement(1256..>1560)
                     /locus_tag="VGB68_12175"
     CDS             complement(1256..>1560)
                     /locus_tag="VGB68_12175"
                     /inference="COORDINATES: protein motif:HMM:NF018599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HEY0050041.1"
                     /translation="LKLKPNCECCDKDLPVESREAMICTFECTFCRDSVETKLNFVCP
                     NCGGDFTARPVRPSNRLAEYPASTERFYKPEGCAGSASRFNWKLIRNSSKINCPNI"
BASE COUNT          338 a          437 c          376 g          409 t
ORIGIN      
        1 cctgaccaaa cttttcgcgc cgtcgcagcc gaccagatat ttcgccgcca tcgtccgagt
       61 ttcgccgtcc gccgtcttaa tcgtcgccgt tacttcatcg ccagcctgcg agatagattc
      121 gagcgcggtt ttccaccgga cgtttttgcc gtgccgctgc aaataatcgt cgagcagtcg
      181 ctcgtttttg ctttgctcca aaagaagcgc gaagggaaac gggcttaaat ccttgccgat
      241 gtccgataaa ttaatcgagc cgcgaatctc gccgtccgcc acgatgtttg ccgtccgggc
      301 tatcgcgccc gcttccaccg ctctttcggc taaatccaac tgctcgtaaa tttccagcgt
      361 gcgcgcctgc acggcgatgg ctttcgacaa atccgtgatg ccctctttac ggtcgaaaat
      421 cacaaagtcg attccgtatc tgattaactg gcaagccaga gccagccccg tcggacccgc
      481 gccgacgata atcacgtccg ttttaattgt ttcaatcata taatttccgg tttacgccga
      541 atcatctcgc atccatttca gagcgataag attttattca gccggcaact gcgcggacga
      601 ataagtccgt aattgccaaa atcttagaac cgccgccgtc ttctgaaaca tttccgattc
      661 aaaacatttt ccgccggtcg gcagcggcgg cgcaagctcg cgccgatttt ttatccggcg
      721 tcgtttatat ccggttccat aaaatcgggc tcggcgcgca ccggaaaatt aacggaattg
      781 gcgataaaac aaagctcgtg cgcccggcgg tgaatttctt tggctttttc agtgtcggat
      841 tcttttgaaa tacggacgcg cggtcttaaa acgacttccg taaaatgccc gccgccgtca
      901 ggcgtctcga ccattttccc cgtcgcgctg tccgtgtaat cggtgacgac aattttcgcg
      961 tcggcgcaca ggtgcaggta ataaagcata tgacaggcgg aaagcgccgc gacgagcaat
     1021 tcttccggat tatagcgcga tgcgtcgccg cgaaaatgcg ggtcgctcga cccgacaatc
     1081 gcgggttttc cttcaatgga aatttcgtgc gcgcggctgt atccggtata agttttcgtg
     1141 ccgtctccca agttccctgt ccagacgaga ttcgtttcgt agatgtgttc tttcggcata
     1201 ttatttccag tttttctttc tgcaataaac ggcggcgata atgtttcccc tgcgtttata
     1261 tattggggca attgatttta gaggaattac gaatcaactt ccaattgaaa cggcttgcgc
     1321 tgcccgcgca gccttccggt ttgtagaatc tttcggtcga agccggatac tcggcaagcc
     1381 ggttcgacgg tctgaccgga cgcgcagtga aatcgcctcc gcagttcggg cagacgaaat
     1441 tgagtttcgt ctcgacgcta tctcggcaaa aagtgcattc gaaagtgcag atcatcgctt
     1501 ctctcgattc aaccggcaaa tctttatcgc agcattcaca attcggtttt aatttcaaca
//
LOCUS       DASWNE010000624         2272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_617743, whole genome shotgun sequence.
ACCESSION   DASWNE010000624 DASWNE010000000
VERSION     DASWNE010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617743"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..>2272
                     /locus_tag="VGB68_12180"
     CDS             44..>2272
                     /locus_tag="VGB68_12180"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing tetratricopeptide repeat
                     protein"
                     /protein_id="HEY0050042.1"
                     /translation="MIENAAAGNDFKSTVGDLKRGLSIFEATGNKEKQAHILSIFCYS
                     YRAAADYEIAAQYHERVIKLYEELKNYKQSVEFINGLGAIKMYQGDFAAAETNHGRAL
                     QIAEAERDAPGIANSLFHLGIIYRARGNYSDSLRVYQRARLIIESLSRDEPKKYLSSL
                     STCLRHLGLVYYLQGINRLALEYFQKSLAIDEATDNSNALAYSLLYIARVYFAENDYP
                     KTLELGERALKIYREAGEKEGTALALNLLGYTQQKLGNFDRALDYFKQSLDIRKSTGS
                     RNGTAVVQTGMANIYQLQGKYAEAVEFASSAVAQFEQDGSRELLWQAQTILGKSYRVS
                     NRREKALAAFNAATGVIENLRRDATGGENERGVFFAGKVEPYQQAALIFAEQSNFAAA
                     FNASEQAKARVLLDVLKFGRVEQSARLTEKEQADEKRLRDEIVSLNTQINRLASQEKA
                     DAKLQADLQIKLEKARLAYTDFQSCAFAAHPELKLQRGEVETVSAKDVSALLDEKTAL
                     LEYLVTGEEILLFVFTKSAGATVLNSYRMPIKRGEIQAKIAVFRQKLATGNLLFAGDA
                     RSLYDLLLKPAREQLSGKTSLVIVPDDGLWELPFQALQSAENHYVLDDAAISYAPSLT
                     ILRELRERRRNQPDALRTLLALGNPSAISGANLKINFAALPEAERQVFALRDLYGGAV
                     SAIYTRAAAREEVFKREAANFRIIHLATHGFLDNNNPLASYVVLSAGSGNTPEDGRLE
                     ARE"
BASE COUNT          555 a          627 c          624 g          466 t
ORIGIN      
        1 cgccgcgcgg gtcggcggca acagcgaatg tcaaggcaat ttaatgattg aaaacgccgc
       61 cgccggtaat gactttaaat cgaccgtggg cgacctgaag cgaggcttat ctattttcga
      121 agcgaccgga aataaggaaa agcaagcgca tattctcagt attttctgtt acagctaccg
      181 ggcggcggct gattatgaaa tcgcggcgca atatcacgag cgcgtcataa aactttacga
      241 agagctgaaa aattacaaac agtccgtcga atttattaac ggtctcggcg ctatcaaaat
      301 gtatcagggc gatttcgcgg cggcggaaac gaatcacggg cgagctttgc aaatcgccga
      361 agcggaacgg gacgcgcccg gcatcgccaa ttcgctgttt catctcggaa ttatctaccg
      421 cgcccgcggc aattacagcg actcgctgcg cgtctaccag agagcgcggc tgataatcga
      481 atctttaagc cgcgacgagc cgaaaaaata tctctcctcg ctttccactt gcctgcggca
      541 cttgggcttg gtttattatt tgcagggcat caaccggctc gcgctcgaat actttcaaaa
      601 atctctcgct attgacgagg caaccgataa cagcaatgct ctggcgtata gcctgcttta
      661 catcgcccgc gtatattttg ccgaaaacga ttatccgaaa actctggagc tcggcgagcg
      721 ggcgttaaaa atttatcggg aggcgggcga aaaagaaggc acggctctcg ccttgaatct
      781 tctcggctac acgcagcaaa agctcggcaa ttttgaccgc gcgctcgatt acttcaaaca
      841 aagtctggac attagaaagt ctaccggaag ccgaaacgga acggcggtcg tacagacggg
      901 aatggcaaac atttatcaat tgcaggggaa atacgccgaa gcggtcgagt tcgcgtcaag
      961 cgccgtcgcg cagtttgagc aggacggcag ccgcgagctg ctctggcagg cgcagacgat
     1021 tctgggaaaa tcctatcgcg tgtcgaaccg gcgcgaaaaa gcgctggcgg catttaacgc
     1081 ggcgaccggc gttatcgaaa atctgcgacg cgacgcgacg ggcggcgaga acgagcgcgg
     1141 cgtttttttc gccggtaaag tcgagccgta tcagcaggcg gctttgatat tcgccgaaca
     1201 aagcaatttc gccgccgcct ttaacgcttc ggaacaagcc aaagcgcgag tgctgctgga
     1261 cgttttgaaa ttcgggcgcg tcgaacaatc ggcgcgtctg accgaaaaag aacaggcgga
     1321 cgaaaaacgc ctgcgcgacg agattgtctc gctcaacacg caaatcaacc gcctcgcctc
     1381 gcaggagaaa gctgacgcga agctgcaagc cgatttgcaa atcaaactcg aaaaagcgcg
     1441 tctcgcctac accgattttc aatcctgcgc ctttgccgcg catcccgaac tgaagctgca
     1501 gcgcggagaa gtcgaaaccg tctcggctaa agacgtctcg gctttgctcg atgaaaaaac
     1561 ggctttgctc gaatatctgg tcacaggcga agaaattctg cttttcgttt ttacaaaaag
     1621 cgcgggcgcg accgtgctta acagctaccg gatgccaatc aaacgcggag aaattcaggc
     1681 gaaaatcgcc gtcttccgcc aaaagctggc gaccggcaat ctgctgtttg ccggcgacgc
     1741 tcgctcgctc tacgatttgc ttttgaaacc ggcgcgcgag caattgagcg gcaaaacctc
     1801 gctcgttatc gtgccggacg acgggttgtg ggaattgccg tttcaggctc tgcaatcggc
     1861 ggaaaatcat tacgttctgg acgacgcggc gatttcttac gcgccgagcc tgacaatttt
     1921 gcgcgagctg cgcgaacgcc gccgcaatca accggacgcg ctcagaaccc tgctcgcttt
     1981 aggtaatcct tcggcgatta gcggcgcgaa tctgaaaatt aatttcgccg cgctgcccga
     2041 agccgagcgg caggttttcg ccctgcgcga tttatacggc ggagcggtaa gcgcgattta
     2101 cactcgcgcc gccgcccgcg aagaggtgtt caaacgcgaa gccgcaaact ttcgcattat
     2161 tcatctcgcc acgcacggtt ttctggacaa taataatccg ctcgcctctt acgttgtgtt
     2221 gtccgccggt tcgggaaata cgccggaaga cggtcggctc gaagcccgcg aa
//
LOCUS       DASWNE010000625         7267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_57250, whole genome shotgun sequence.
ACCESSION   DASWNE010000625 DASWNE010000000
VERSION     DASWNE010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7267
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57250"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VGB68_12185"
     CDS             complement(<1..242)
                     /locus_tag="VGB68_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050043.1"
                     /translation="MLKITSRDNQKIKFARQVRDGRERDFVFVEGARLAEEVLKAGLP
                     IREVFFTASFAESERGRSFLKSVEAKSESAAEVSGK"
     gene            336..408
                     /locus_tag="VGB68_12190"
     tRNA            336..408
                     /locus_tag="VGB68_12190"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:369..371,aa:Arg,seq:ccg)
     gene            complement(830..1459)
                     /locus_tag="VGB68_12195"
     CDS             complement(830..1459)
                     /locus_tag="VGB68_12195"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY0050044.1"
                     /translation="MKILKFILLPALFLTLFACSQNQSTGNKSGNKNAAVKKGVVPVA
                     DPEVAVIEMESGGAFGKITIELYSNVAPEAVKRFKELAREGFYNGTSFHRINATVIQG
                     GDPLSKDADPSNDGTGQSDKPNVKAEFSDIPYDTGIVGAARSQDVDSANSQFFIMLKR
                     EPGFDNNYTVFGKVVDGMNNVKTIAGVPRSGERPMDDVKIKSVTIQSKQ"
     gene            complement(1681..2064)
                     /gene="dtd"
                     /locus_tag="VGB68_12200"
     CDS             complement(1681..2064)
                     /gene="dtd"
                     /locus_tag="VGB68_12200"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR00256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEY0050045.1"
                     /translation="MRAVLQRVSRASVTVGDEMVGEIGRGILVFLGVARGDTEKDALY
                     LLEKTLNLRIFEDDEGKMNLSLLDISGEFLVVSQFTLYGDVRKGRRPSLSKPPRPSKP
                     IACMNFSRLKPESKLRKSKPENFRR"
     gene            complement(2187..2678)
                     /locus_tag="VGB68_12205"
     CDS             complement(2187..2678)
                     /locus_tag="VGB68_12205"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY0050046.1"
                     /translation="MANRIAVIETNKGTIKFELLEQDAPKTTENFRLLAEKGYYDGVI
                     FHRVIKNFMIQGGDPLGMGYGGESAWGGKFNDEINKTSALYQGGYQKGTVAMANSGPN
                     TNGSQFFIMHIDYPLPPSYTKFGRVIEGQDVIDAIADVQTNSSDKPLEPVVMNKVTIE
                     EQS"
     gene            complement(2744..3610)
                     /locus_tag="VGB68_12210"
     CDS             complement(2744..3610)
                     /locus_tag="VGB68_12210"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEY0050047.1"
                     /translation="MIKLLALDLDGTLLDSKGKIPDANLQAIRAAEEKGVLVTIATGR
                     RFRDARPLGLQLDLNAPLITHNGALLKYAQTLETVAVSLLSGETAREILRVGREFGAD
                     ALLSCDPHGKGTLLYERVSAENIPLQKYIVWSKHLHGDEAEEAVLHVSNLENVLPETE
                     TVHVSFSGRCAPMGELEMILQNELSDTATILTTRYPRLDFTLIDILPPDASKGIGLEK
                     LAVIENLTPENVMVVGDNFNDLQMLEFAGTPVVMGNAAPELLENANFHQTLSNDENGV
                     AAAIERFILSEN"
     gene            complement(3607..4155)
                     /locus_tag="VGB68_12215"
     CDS             complement(3607..4155)
                     /locus_tag="VGB68_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050048.1"
                     /translation="MTPNELLSPAEHKLFFGIAFLICCLFFINLTNKTIDSYNEAVEK
                     AQIEKRAAADNKPKISFGLYHCFYSIPLYEVVLALQFFTNPLLLLLSLKRGTGRFIGS
                     GLLTAFTFSGYACWLTRTYWAIKYDELFYRADMSFNNYLLYNSTAAEFTLFLIFAVLL
                     VLQICVPTRFVIEKFQARIYLR"
     gene            complement(4216..4440)
                     /locus_tag="VGB68_12220"
     CDS             complement(4216..4440)
                     /locus_tag="VGB68_12220"
                     /inference="COORDINATES: protein motif:HMM:NF025742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEY0050049.1"
                     /translation="MKDDDIDLSDCPEIMPEMFAKAIVRKDFKKIAGKSQLTLRIDQD
                     VIEFFKAQGRGYQTKINHLLRAYMEAHRQK"
     gene            complement(4460..4735)
                     /locus_tag="VGB68_12225"
     CDS             complement(4460..4735)
                     /locus_tag="VGB68_12225"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEY0050050.1"
                     /translation="MRFEWDEEKRRINLTRHGFDFVDAEQVFENETATILDDRFDYGE
                     IRFVSFGLLKGFVVAVVPNQVKEITRIISFRKATKNEERYYFEKIRN"
     gene            complement(4765..5838)
                     /gene="mltG"
                     /locus_tag="VGB68_12230"
     CDS             complement(4765..5838)
                     /gene="mltG"
                     /locus_tag="VGB68_12230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEY0050051.1"
                     /translation="MRFLKILVLLFILAALAGGGAAFWLYKSLNAAHEHSKSNQFIVI
                     PKGSTPNEIIDKLAAEGILANPLSTQIYLRSFGDAGKLKAGEYQFESPITPLQVLQEL
                     EKGEERTVKLTIPEGWTRFDIAKRLAEKFPTNPPMDEKAILYLMDDVSLIKDFDANAK
                     NLEGYMYPSTYSLPKGSTPQQAIKTMVEHFKKTWKPEWNERARALGRTPHEIVTIASL
                     IETESKFDNERTIVASVIYNRLNKGIALGIDQTAVYVAKMQNRWDGTINKSDLEVDSP
                     YNTRKYAGLPPAPISSVSESALNAALNPAQTDYIYYVLNVEKNDGSHHFYASASEFER
                     GKAAYQIWLEQQRAKKREQETNN"
     gene            5994..6572
                     /gene="thpR"
                     /locus_tag="VGB68_12235"
     CDS             5994..6572
                     /gene="thpR"
                     /locus_tag="VGB68_12235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEY0050052.1"
                     /translation="MKRIFAAIDISEQARRKAFEYIESLRRRFPELRVGWDKYEKLHL
                     TLKFLGDTSDERLAKLEKAVEKAAREVSSFKLRLADTGVFPNQRKARVLWLGVKDEKA
                     NLRKLNEALENECEREGFTRENRDFKAHLTIARLREPEKSKALVEAHLREQFEAVEFE
                     VSEIVIYQSELSPRGSRYTVVSKHAFDLKEEN"
     gene            complement(6654..6941)
                     /locus_tag="VGB68_12240"
     CDS             complement(6654..6941)
                     /locus_tag="VGB68_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050053.1"
                     /translation="MPTTLTQLEDTERGKTILRVEGSMTYDDAVLLEKIALDLRSQMD
                     RNLTIDLADLHFLDSDSAPIIKRIEREHGFEIEGIEVFLQNAVSEAEGKSF"
BASE COUNT         1765 a         1772 c         1768 g         1962 t
ORIGIN      
        1 attttgccgg agacttccgc cgcgctttcg cttttcgctt cgaccgactt taaaaaactc
       61 cgcccgcgtt ccgattcggc aaagcttgcc gtgaaaaaaa cttcgcgaat cggcagcccg
      121 gctttcaaaa cttcttcagc cagccgcgcg ccttcgacaa aaacgaaatc gcgctcgcgc
      181 ccgtcgcgta cctgacgcgc aaattttatc ttttgattat cgcggctggt gattttcagc
      241 atcggaaaat tccgtcgaaa ctaaaaacta actttcaggc gagttaattt caggcggttg
      301 tgcgtcgctt tggtttatgc cataatttct tcgctgcgcc cgtagctcag tggatagagt
      361 gttcggctcc gaaccgaaag gtcgcaagtt caagtcttgc cgggcgtatt tatttttcaa
      421 ctcttccaga tgcttttccc ataaataatc ggacgcccat cgaataagct ttgattgccc
      481 gttttttaca ggctcaagac gaagccagcc cagccccaga tgattctcgg cgggaattcc
      541 gtaaagcgaa tcattagccg gacaatagac ggcaatgtaa tcgacctcgc cttttttata
      601 aggtcgccgc gcttcgctct tcggttgtcg gcgcaggcat ttatatttca ggacgccttt
      661 gctgagccag gctgttttaa cctgaatctt gaaaatgtcc gttcccgcat cgaccagcaa
      721 atcgtagctc gcaccggagc cgaatggaat acttaccgta aaacccgctt ttatataagc
      781 gctcaaaatg atcgattcgc tgcgattgcc gatttcatac ttttgcatat tactgcttcg
      841 attgaatcgt cacggacttg attttcacgt cgtccatcgg gcgctcgccg ctgcgcggga
      901 cgccggcaat cgttttgacg ttgttcatcc cgtcgacgac tttgccgaaa accgtgtaat
      961 tattatcaaa ccccggctcg cgtttcagca taataaaaaa ctgcgagttt gccgaatcga
     1021 cgtcctgcga gcgcgccgcg ccgacgattc ccgtatcata cggaatatcg gaaaactcgg
     1081 ctttgacgtt cggtttatcc gattgaccgg taccgtcatt gctcgggtcg gcgtctttcg
     1141 agagcgggtc gccgccctga atgaccgtag cgttgatgcg gtgaaacgac gtgccgttgt
     1201 aaaagccttc gcgcgccagt tccttaaagc gcttgacggc ttcgggcgcg acgttcgagt
     1261 aaagctcaat cgtgattttg ccgaacgcgc cgccgctttc catttcgatg acggcgactt
     1321 ccgggtcggc gacgggcacg acgccttttt tgacggcagc gtttttgttg ccggatttat
     1381 tgcctgtcga ttggttttgg ctacaggcaa aaagcgtcag gaaaagcgcc ggtaataaaa
     1441 taaatttcaa aattttcata gatttaatcg gaattttaaa accacagatt atcacggatg
     1501 aatttacagg gataacggcg attgtcagcg atatataaaa attatccggc ttaatccccg
     1561 ctcatctcag ttatccgcgc ggatttcttt ttccgtccgc tcacggcggt caatttcaaa
     1621 aaagcttcgc gctgtcgagc agaatcgtca ccggaccgtc gttgacgagc cggacgtcca
     1681 tcatcgcctg aaattttccg gtttcgactt tcgcaatttg ctttcgggct tcagccgcga
     1741 aaaattcata caggcgattg gcttcgacgg gcgcggcggc ttcgataaag aaggtcgcct
     1801 gcccttgcgc acgtcgccgt agagcgtgaa ctgtgaaacg acgagaaatt ctcccgaaat
     1861 atccagcagc gaaagattca ttttgccttc gtcgtcttca aagatgcgaa gattcaacgt
     1921 tttttctaac agataaaggg cgtccttttc cgtatcgccg cgcgcgacgc cgagaaaaac
     1981 taaaattccc ctgccgattt cgccgaccat ctcgtcgccg actgtaacgc tggcgcgcga
     2041 gactctctgc aaaactgctc tcataaagcg gtaatgtgaa aaagtgaaag agtgaaagag
     2101 tgaaagagtg aaagagtgaa aaagcgcgtc gatttccgtc agccaaactt tttcaccttt
     2161 ccgctttttc actctttcac ttttttttaa ctttgctctt ctatggtgac tttgttcata
     2221 acgaccggtt cgagcggttt atcgctggaa ttggtttgaa catcggcaat cgcgtcgatt
     2281 acgtcctgtc cttcgatgac gcgaccgaat ttcgtatagc tcggcggcag cggataatcg
     2341 atgtgcataa taaaaaactg cgagccgttc gtattcggtc ccgaatttgc catcgccacc
     2401 gtgccttttt gataaccgcc ctgatagagc gcggaagttt tattgatttc gtcgttaaac
     2461 tttccgcccc acgccgattc gccgccgtag cccattccga gcgggtcgcc gccctgaatc
     2521 ataaagtttt tgataacgcg gtgaaaaatc acgccgtcgt aataaccttt ttccgccaga
     2581 aggcgaaaat tttcagtcgt tttcggcgcg tcctgttcga gtaattcaaa tttgatagtg
     2641 cctttattgg tttcgataac cgcaattctg tttgccatta ataaaatctc cttgaaatat
     2701 aaaatcaaag attatcaaaa aatgagcgca gatagaaact catctaattt tcgctcaaaa
     2761 taaatcgctc gattgccgcg gcgacgccgt tttcgtcgtt cgataaggtt tgatgaaagt
     2821 tggcgttttc cagcagttcg ggcgcggcgt tgcccatcac gaccggcgtt ccggcaaatt
     2881 cgagcatctg caaatcgtta aaattatcgc cgacgaccat cacgttttcc ggcgtcagat
     2941 tttcgatgac ggctaatttt tccagcccga tgcctttcga ggcgtctggc ggcagaatgt
     3001 caatcagcgt gaaatccaga cgcgggtaac gggtcgtcaa aatcgtcgcc gtatcgctta
     3061 attcattttg cagaatcatt tccagctcgc ccatcggcgc gcatctgccc gaaaaggaaa
     3121 cgtgcacggt ttccgtttcg ggcaaaacat tttccagatt agagacgtgc agcacggctt
     3181 cttcggcttc gtcgccgtgc aggtgtttcg accagacgat gtatttttgc agcggaatat
     3241 tttcggcgga gacgcgctcg taaagcagcg tgcccttgcc gtgcgggtca cagctcaaaa
     3301 gcgcgtcggc gccgaactcg cgcccgacgc gcaaaatttc ccgagcggtt tcgccggata
     3361 aaagcgaaac cgcgaccgtt tccagcgttt gcgcgtattt caagagcgcg ccgttatgcg
     3421 taatcagcgg cgcgttcagg tcgagctgca agccgagcgg acgcgcgtcg cggaaacgcc
     3481 tgccggtggc gattgtcacc aaaacgcctt tttcttcggc ggcgcggatg gcttgcagat
     3541 tggcgtcggg aattttgcct tttgaatcga gcaaagttcc gtcgaggtcg agcgcgagaa
     3601 gttttatcat ctgagataaa ttctagcttg aaatttctcg atgacaaaac gcgtcggaac
     3661 gcagatttgc agaacgagca aaaccgcgaa aatcaaaaac agagtaaact ccgcagcggt
     3721 cgaattataa agcagataat tgttgaaact catatcggcg cgataaaaca attcgtcgta
     3781 ttttatcgcc cagtaagtgc gagtcagcca acaggcgtag ccggagaagg taaaagcggt
     3841 cagcagaccg gaaccgatga atcttcccgt cccgcgcttt aaagacagta gaagcaaaag
     3901 cggattcgtg aaaaactgaa gcgccagaac gacctcgtag agcggaatcg agtaaaagca
     3961 atggtagagc ccgaaactga ttttcggctt gttatctgcg gcggctcttt tttcaatctg
     4021 tgctttttca accgcttcgt tgtagctgtc aatagtttta tttgtcagat ttataaaaaa
     4081 caggcagcaa atcaaaaatg cgattccgaa gaaaagcttg tgttcggcgg gcgaaagtaa
     4141 ttcgttcggc gtcataactt tgaaatctct cccgtttagt tagacaccga attttcgata
     4201 aagttccgac ccgatttatt tctgccggtg cgcttccata taagccctca gcaggtggtt
     4261 gatttttgtt tgatagccgc gaccttgcgc cttaaaaaat tcgataacgt cctgatctat
     4321 tcgcagagtc aattgcgatt tgcccgcaat cttcttgaag tctttgcgaa caatggcttt
     4381 agcgaacatt tccggcatta tttcaggaca atccgataaa tcaatgtcat cgtccttcat
     4441 attttttatt cgctcccaat cagtttcgga ttttttcaaa gtagtatctt tcttcatttt
     4501 ttgtagcctt tctgaaagag ataattcttg ttatttcttt aacttgatta ggaacgacag
     4561 cgacgacgaa acctttaagt agtccgaagc ttacaaatcg aatttcgccg taatcaaatc
     4621 ggtcatcgag gattgttgcc gtttcattct cgaatacctg ctctgcatcg acaaaatcga
     4681 atccgtgtct cgttaaattt atccggcgtt tttcttcatc ccattcaaat ctcataaaaa
     4741 ttgcagttac attttgtagc tacgtcaatt atttgtttcc tgctcgcgct ttttcgctct
     4801 ttgctgttcc agccagattt gatacgcggc ttttccgcgt tcgaattccg atgccgacgc
     4861 gtagaaatga tgcgagccgt cgtttttctc gacgtttaaa acgtaataaa tataatcggt
     4921 ctgcgcggga ttcagcgcgg cgttcagcgc gctttccgag accgaggaaa tcggcgcggg
     4981 cggcagcccg gcgtatttgc gcgtgttgta gggcgaatcg acttccagat cgcttttgtt
     5041 aatcgtgccg tcccagcggt tctgcatttt cgccacgtaa accgccgtct ggtcgatgcc
     5101 gagcgcgatt cctttgttca ggcgattgta gatgacggaa gcgacaatcg tgcgctcgtt
     5161 gtcaaatttc gattcggttt caatcaagct ggcaatcgtc acgatttcgt gcggcgttcg
     5221 ccccagcgcg cgcgcgcgct cgttccattc cggcttccag gtttttttaa agtgctcgac
     5281 catcgttttg atggcttgct gcggcgtcga gcctttcggc aggctgtaag tgctcggata
     5341 catataacct tcgagatttt tcgcgttcgc gtcgaaatct ttgataaggc tgacgtcgtc
     5401 catcagatac aaaatcgctt tttcgtccat cggcggattg gtcggaaatt tttcggcgag
     5461 gcgtttggca atgtcgaagc gcgtccagcc ttcgggaatc gtgagcttga cggtgcgctc
     5521 ctcgcctttt tccagttcct gcaaaacctg caagggcgta atcggcgatt cgaattgata
     5581 ttcgcccgcc tttaatttgc ccgcgtcgcc gaacgagcgc aggtaaatct gcgtcgaaag
     5641 cgggttggcg agaatgcctt cggcggcgag cttgtcgata atttcgttcg gcgtcgagcc
     5701 tttcggaatg acgataaatt gattcgattt gctgtgctcg tgcgcggcgt tcagcgattt
     5761 gtaaagccag aaagccgcgc cgccgcccgc caaagccgct aaaataaaga gtaaaacaag
     5821 aatttttaag aatctcattg tggaaaagtt tagcaaaaaa tgagaatttc aaccacagag
     5881 taaagcgatg ccgaaacgta aaaggcaaaa ggcaaaagtg aaaaatcaat ttctttgttt
     5941 tccgctctgc cttttacctt ttgcgccgaa ctatctgtgg taaattttct tatatgaaac
     6001 gaatttttgc ggcgattgat atttccgaac aggcgcgccg caaggctttc gaatatattg
     6061 aaagcctgcg gcggagattt cccgaattgc gcgtcggttg ggacaaatat gaaaagctgc
     6121 acctgacgct gaaatttctg ggcgatacga gcgacgagcg gctggcaaaa ttggagaaag
     6181 ccgtcgaaaa agcggcgcgc gaagtttcga gcttcaaatt gcgtcttgcc gacacgggcg
     6241 ttttcccgaa ccagcgaaaa gcgcgcgttt tgtggctcgg cgtgaaagac gaaaaggcaa
     6301 atttgagaaa attaaacgaa gctctggaaa acgaatgcga gcgcgagggt tttacgcgag
     6361 aaaacaggga ttttaaagct cacttgacga ttgcgcgtct gcgcgagccg gaaaaatcaa
     6421 aagccctggt cgaagcgcat ttgcgcgaac aatttgaagc cgtcgaattt gaagtttccg
     6481 aaatcgtgat ttatcaaagc gagctttcgc cgcgcggctc gcgctatacg gtcgtttcaa
     6541 aacacgcttt cgatttaaag gaagaaaatt aacagggata aaggggatag gcaaggataa
     6601 aaacaatttt ttaaatccct gtccatccct ttcatccctg ttaatttttc tgtttagaaa
     6661 ctcttgccct cggcttcgga aacggcgttt tgcagaaaaa cctcgatgcc ttcgatttca
     6721 aagccgtgtt cgcgctcgat gcgtttgata atcggcgcgg aatccgaatc gagaaaatgc
     6781 aaatccgcca aatcaatcgt cagattcctg tccatctgcg agcgcaaatc gagcgcgatt
     6841 ttttccagca aaacggcgtc gtcataggtc atcgagcctt cgacgcgcaa aatcgtcttt
     6901 ccgcgttccg tgtcttcgag ctgggttaaa gtagtcggca tttgtttgct gggatttaac
     6961 gaaaaaaaag cgataaaatc aactgcaatt tattcttact gtttataaag acacaaaaac
     7021 gatgcaaaag gtttcatttt tttttcattg ctggttgaat gtagattgga gtttgtattt
     7081 tcgcgccgcc ggtaaaggac aaaggacaac aattaattta tgtctcggat tttattgatt
     7141 gacgatgacg aagagctttg cgagctggtt tcggagtatt tgacggtcga aggttttacc
     7201 gtcgaagcga ttcacgacgg cgagacgggc ttgcagaagg cgaaagcggg cgggcacgat
     7261 ttgatta
//
LOCUS       DASWNE010000626         3235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_126164, whole genome shotgun sequence.
ACCESSION   DASWNE010000626 DASWNE010000000
VERSION     DASWNE010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126164"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /gene="recF"
                     /locus_tag="VGB68_12245"
     CDS             <1..1112
                     /gene="recF"
                     /locus_tag="VGB68_12245"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HEY0050054.1"
                     /translation="EVQNFRNLQGKFFCREGLNVIFGENGQGKTNWLEAIYTLATTKS
                     FKTPKLQEAIRFNEELAIIRGTVRQSAEIHRAMQVALQGNTKTLSVNAKKETIQRYLG
                     QLHTVIFNADELEIVRGLPDSRRRFLDAGITAIYPPFVQTIADYNRVIRQKNSLLQSA
                     QENEFSLEKTAEMLEPWNEQLIALATRIHKARIRYVERLNEVLEKRLFGKEELSIRYA
                     SSLEGKGDLSDYAALLAERLKLRVQAEVVSGHALIGTHRDDLDILFDGHDLRKFGSSG
                     QQRSALLILQLANLSVYYSQHREYPLFLLDDIDAELDYKRIGQLLEYLSDKTQTFVTT
                     SKESFVEKFGASAKIFKVSEGKILDEEQEFLSSFP"
     gene            1190..1858
                     /locus_tag="VGB68_12250"
     CDS             1190..1858
                     /locus_tag="VGB68_12250"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY0050055.1"
                     /translation="MQKNGRIDLSFFLIVRRSSVPGKARFYKAFLVLIFTFTFGLFSF
                     GQETKEGANPAWQKGISGAKVRIEVFNDYQCPTCAAFDKNLDDILRKYPNEVLIIYRN
                     FPLTGMHRNAMAAAKAVEAAGKQGKFWEMMRLVYKNQAKWSESPSAEADFVKYAKKLR
                     LNIETFKTDRESESVTSRINADIERSRFLELSATPTVVFNGRILSAMETGYLEKIIEK
                     QLSK"
     gene            1874..>3235
                     /locus_tag="VGB68_12255"
     CDS             1874..>3235
                     /locus_tag="VGB68_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0050056.1"
                     /translation="MAKAKTEYGADSITVLEGRDAVRKRPAMYIGSTSEIGLHHLVYE
                     VVDNSVDEALAGYCDTIEVAIHIDDSITVIDNGRGIPTDIHKQEGRSAAEVVMTILHA
                     GGKFDSNSYKVSGGLHGVGVSCVNFLSEWLKLEIWRDGKTFEQEYTRGIPNAPLKQTG
                     KTEKRGTKITFKPDSEIFETTLYSFEKLSERLREKAFLNKGIRIFIKDEREETEKSHE
                     FYYKGGIAEFVRHLNKNKSVLHDEPLYFEQIADDLSVEVSMQYNDSYDEKIFSFANNI
                     NTVDGGTHLSGFRGAITRTINNYAESSGLLKNSKTILTGDDVREGLVAVISVKIPQPQ
                     FEGQTKGKLNSPVKGPVESFLNEKLGEFFEQNPNVAKKIVGKAVDAARARDAARKARE
                     IVRKSALGGSTLPGKLADCQEKDPALSEIYLVEGDSAGGSAKQGRDRKNQAVLPLKGK
                     ILNV"
BASE COUNT          924 a          735 c          839 g          737 t
ORIGIN      
        1 tagaagttca gaatttccgc aatttacaag ggaaattttt ttgccgcgaa ggcttaaacg
       61 taattttcgg cgagaacggg cagggaaaaa ccaactggct cgaagcgatt tacacgctgg
      121 cgacgacgaa atcttttaaa actccgaaat tgcaggaagc cattcggttc aacgaagaac
      181 tcgccattat tcgcggcacg gttcggcaat cggcggaaat tcaccgcgcg atgcaggtcg
      241 cgcttcaggg aaacaccaaa actctttcgg tcaacgcgaa aaaggaaacg attcagcgct
      301 atctcggaca gcttcacacg gtcattttca acgccgacga gctggaaatc gtgcgcgggc
      361 ttccggattc gcgacgccgc tttctagacg cgggcatcac ggcgatttat ccgccgttcg
      421 tgcagacgat tgccgactac aaccgcgtga ttcgacagaa aaattcgctt ttgcaatcgg
      481 cgcaggagaa tgaattcagc ctggagaaaa cggcggaaat gcttgagccc tggaatgagc
      541 agctgattgc cctggcgacg cggattcaca aagcgcggat tcgttatgtc gaaagactga
      601 acgaagtttt ggaaaagcgc ctgttcggta aagaggagct gtcgattcgc tacgcctcgt
      661 cgctggaagg caaaggcgat ttgagcgatt acgccgcgct tttagccgag cgcttaaagc
      721 ttcgcgtgca ggcggaagtc gtttccggac acgcgcttat cgggacgcat cgcgacgatt
      781 tggatatttt gtttgacggt cacgatttgc gaaaattcgg ctcgagcggg cagcagagaa
      841 gcgcgctgct tattttgcag cttgccaatc tttcggtcta ttactcgcag caccgggaat
      901 acccgctgtt tttgctcgac gacatcgacg ccgaactgga ttacaagcgc atcggtcaac
      961 tgctcgaata tctaagcgat aaaacccaaa ctttcgtcac gacctccaaa gaaagctttg
     1021 tcgaaaaatt cggagcttca gcgaaaattt tcaaggtttc ggaaggcaaa attctcgacg
     1081 aggaacagga atttttgagc agtttcccat aatcggaaaa actcgtttta aattatcctc
     1141 aaaactaatt ataaactcgt aaaaatcagc tctttgcggc tattattttt tgcagaaaaa
     1201 tggtagaata gacttaagtt ttttcctcat tgtaaggaga agttcagtgc ccggaaaagc
     1261 gcgtttttac aaagcatttt tagttttgat ttttactttc acattcggtc ttttttcttt
     1321 cggacaagag actaaagaag gagcaaatcc ggcgtggcaa aaaggaattt cgggcgcgaa
     1381 agtgcgcatt gaagttttta acgactatca atgtccgact tgcgccgcgt ttgacaaaaa
     1441 ccttgatgat attttgcgga aatatccgaa tgaagttttg attatttaca gaaattttcc
     1501 gcttaccgga atgcatagaa atgcaatggc tgccgcgaaa gcggtcgagg ctgccggaaa
     1561 gcagggaaaa ttttgggaaa tgatgcggct cgtttataaa aatcaagcta aatggagtga
     1621 aagcccgtcg gctgaagcag attttgttaa atacgctaaa aaactccgat tgaatattga
     1681 aacttttaaa accgaccgcg aaagcgaatc ggtgaccagc agaatcaatg ccgatattga
     1741 acgttcccgc tttctggaat tatcagcgac gcctaccgtc gtttttaatg gcagaatatt
     1801 aagcgctatg gaaacgggat atttagaaaa aattatagaa aaacaattaa gtaaatagaa
     1861 agaggttaat aaattggcta aagcaaaaac agaatacgga gcagattcga ttaccgtttt
     1921 ggaaggacgc gacgcggtgc gcaagcgccc ggcgatgtat atcggctcga ccagcgaaat
     1981 cggtctgcat catcttgttt acgaagtcgt ggacaactcg gtggacgaag cgctcgccgg
     2041 ttactgcgat acgattgaag tggcaatcca cattgatgat tcgattaccg tgatagacaa
     2101 cggacgcggc attccgaccg acattcacaa acaggaaggt cgctcggcgg cggaagtcgt
     2161 catgacgatt ctgcacgcgg gcggcaaatt cgactcgaat tcgtataaag tttccggcgg
     2221 gctgcacggc gtcggcgtct cctgcgtcaa ctttctgagc gaatggctga agctggaaat
     2281 ctggcgcgac ggcaagactt tcgagcagga gtacacgcgc ggaattccga acgcgccgct
     2341 caaacaaacc ggcaaaaccg aaaaacgcgg cacgaaaatc acgttcaaac ccgacagcga
     2401 aatttttgaa accacgcttt acagctttga aaaactgtcg gaacggctgc gcgaaaaagc
     2461 gtttttgaac aaaggaatcc gcatttttat caaagacgaa cgtgaagaaa cggaaaaatc
     2521 tcacgaattt tattacaaag gcggcatcgc cgaattcgtc aggcatttaa acaaaaacaa
     2581 gagcgtgctg cacgatgaac cgctttattt cgagcagatt gccgacgatt tatcggtcga
     2641 agtctcgatg cagtataacg acagctacga cgaaaagatt ttctcgtttg ccaacaacat
     2701 caacacggtc gacggcggaa cgcatttatc gggctttcgc ggggcgatta cgcgcacgat
     2761 taataattac gcggaaagtt ccggcttgct gaaaaactcc aaaaccatcc tgaccggcga
     2821 cgacgtgcgc gaaggcttgg tcgccgtgat ttcggtgaaa attccgcagc cgcagttcga
     2881 gggtcagacg aagggaaaat taaattcgcc ggtgaaaggt ccggtcgaat catttctgaa
     2941 cgaaaagctc ggcgaatttt tcgagcagaa cccgaacgtg gcgaagaaaa tcgtcggcaa
     3001 agcggttgac gcggcgcggg cgcgcgacgc ggcgcgaaag gcgcgtgaaa tcgttcgcaa
     3061 gagcgcattg ggcggctcga ccttgcccgg aaagcttgcc gattgtcagg aaaaagaccc
     3121 ggcgctttcg gaaatttatc tggtcgaagg cgattcggcg ggcggttcgg cgaaacaagg
     3181 tcgcgataga aaaaatcaag ccgttttgcc gctcaaagga aagattctga acgtc
//
LOCUS       DASWNE010000627         3584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_269585, whole genome shotgun sequence.
ACCESSION   DASWNE010000627 DASWNE010000000
VERSION     DASWNE010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269585"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /gene="dcm"
                     /locus_tag="VGB68_12260"
     CDS             complement(<1..481)
                     /gene="dcm"
                     /locus_tag="VGB68_12260"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:TIGR00675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEY0050057.1"
                     /translation="MSNQLLVRDVPEQIRTWIEEERNNRHMTQKDFVLSVLHQAHENS
                     SIQTKLPFEPQHIEPSLVGRIPFSFIDLFAGIGGFRIPLEGIGGNCVFSSEIDKYSQK
                     TYKAWFDETPHGDITKINATDIPDHDVLAAGFPCQPFSLAGVSKKNSLGHAHGFNCEI
                     "
     gene            948..1265
                     /locus_tag="VGB68_12265"
     CDS             948..1265
                     /locus_tag="VGB68_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050058.1"
                     /translation="MNENSTKDKEAKMRDKEWHLDVSEEQYEEMKARGLDEESLFKPG
                     RHTFRRRDASKIINRDNKTIILHLDDETFDYYQRRAEEKQSASIEEQIEIELRNIAEK
                     EAA"
     gene            1281..2876
                     /locus_tag="VGB68_12270"
     CDS             1281..2876
                     /locus_tag="VGB68_12270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEY0050059.1"
                     /translation="MTAERRERFETSSGIELPNDYNPSNSDFDYETDLGEPGRFPYTR
                     GVRRNMYRGKFWTMRQYAGFATAEESNARYKYLLSQGTTGLSVAFDLPTQIGLDSDDA
                     LAAGEVGKVGVAIDSLEDMLTLFGGIPLDQVSTSMTINATASTLLCLYLAVARKQNVA
                     FDKVNGTIQNDILKEYIARGTYIYPPKPSLRLITDTFAFCAAEVPNWNTISISGYHIR
                     EAGSTAAQEIAFTLADGIAYVDAAISVGLEVDDFAPRLSFFFNAHNNLLEEIAKFRAA
                     RRLWAKLMRERFKAQNPKSLMLRFHTQTAGSTLTAQQPEVNVVRTTIQALAAVLGGTQ
                     SLHTNSMDEALSLPTENAARIALRTQQVIAHEAGVADTVDALAGSYAIEELTNRLEQK
                     AGEYIEKIDAMGGMLRAIEQGYVQREIQEAAYEYQRAVEKQTAIVVGVNKFQMEETET
                     IPIQRVNPKIEREQIERLQAVRASRDAAKAENALAKLEESANGTENLLPRILDCVESR
                     VTVGEISHRLRKVWGEYREAVTV"
     gene            3034..3453
                     /locus_tag="VGB68_12275"
     CDS             3034..3453
                     /locus_tag="VGB68_12275"
                     /inference="COORDINATES: protein motif:HMM:NF016024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rad52/Rad22 family DNA repair protein"
                     /protein_id="HEY0050060.1"
                     /translation="MAEERNVEELDSKNESTELRSIRDIVRDLSKPVAKRHLRTRKQG
                     GKELTYISWYDAVKYLDHYAPGWCYEVRSINSISGKLILTIRLSSPCQEGVIYREATG
                     QEEEETTSYGDSSSNAESMALRRAAAKFGLGLYLYDQ"
BASE COUNT          950 a          865 c          967 g          802 t
ORIGIN      
        1 gaatttcaca attaaaaccg tgcgcgtgtc ctaaactatt cttttttgac acacctgcga
       61 gtgaaaaggg ttgacacgga aatccagccg ctaatacatc atggtcggga atatctgtgg
      121 cgtttatttt agtaatgtcg ccgtgcggag tttcatcaaa ccatgctttg taagttttct
      181 gggaatattt atctatttcg gatgagaaaa cacaattgcc gccgattcct tcgagtggaa
      241 tccgaaagcc tccaataccg gcaaataaat caataaaact gaaaggaatt cgaccaacga
      301 gtgaaggttc aatgtgttgt ggttcaaatg gcaacttggt ctgaatacta ctgttttcgt
      361 gcgcctgatg aagaacagaa agaacaaaat ctttctgcgt catatgtctg ttatttcgct
      421 cctcctcaat ccaagtgcga atttgttccg gcacatctct aactaaaagc tggttactca
      481 tttttattct ctctgggaaa aattgatagc agaatagcat attttcccag agagcgtgaa
      541 gctgattttt ttgaaatatc cacttgtctt aatttactcc gtcaattggg attttgtgtt
      601 gcttagatta acttttcagc ccgtatgttt tcgcggttcg attatgctaa tctattggcg
      661 ttatgacagc agaaagacgc gagagatttg aggtaaaata gaacttgatt tttgaatggg
      721 acagtaccaa atccgaagaa aatttaaaaa agcacggcgt cagctttact gaagccgaag
      781 tcgctttgaa gatttttatg cgattgagga atttgatgac gaacattcaa ccgccgcaga
      841 agcaggatat aaaatgccgg cgctggcgaa ggcaaaaatc ttagttatag tttacgctat
      901 gcgcggtgaa aactttcgca ttatctcggc gcgagaggcg gaaaaatatg aacgagaact
      961 ctacgaaaga caaagaagcg aagatgcgcg ataaagaatg gcatctggac gtgagcgaag
     1021 agcagtatga agaaatgaaa gccagaggtt tggatgagga atctttgttc aagcccggca
     1081 gacatacatt tcgccggcgc gacgcgagca agattattaa tcgcgacaac aaaacgatta
     1141 tcctgcatct ggatgatgaa actttcgatt attatcagcg ccgcgctgag gaaaagcagt
     1201 cagcgtcaat cgaagaacag atagaaatag agcttcgtaa tatagccgag aaagaagcgg
     1261 cttagttaaa tcttaaaaat atgacagcag aaagacgcga gagatttgag acttcttcgg
     1321 gcattgagct gccgaatgat tataacccgt cgaattcgga tttcgattac gagacggatt
     1381 tgggcgagcc gggcaggttt ccgtatacgc gcggcgtcag gcggaatatg tatcgcggga
     1441 aattctggac gatgcgccag tacgccggtt ttgcgaccgc cgaggaatcg aacgcgcgtt
     1501 ataaatatct tttgtcgcag gggacgaccg gcttgagcgt ggcgtttgat ttgccgacgc
     1561 aaatcgggct ggattcggac gacgcgctgg cggcgggcga ggtcggcaag gtcggcgtgg
     1621 cgatcgattc gctcgaagat atgctgactt tgttcggcgg cattccgctc gaccaggttt
     1681 cgacttcgat gacgataaac gcgacggctt cgacgctttt gtgtctttat ctggcggtgg
     1741 cgcgcaagca aaacgtcgcg ttcgacaagg tcaacggcac gattcaaaac gatattttga
     1801 aggaatacat cgcgcgcgga acgtatatct acccgccgaa gccttccttg agattaatca
     1861 ccgacacttt cgctttctgc gccgccgaag tgccgaactg gaacacgatt tccatcagcg
     1921 gctatcacat ccgcgaagcc ggttcgaccg ccgcgcagga aatcgccttc actctggcgg
     1981 acggcatcgc ttatgtcgac gcggcgattt cggtcggtct ggaagtcgac gacttcgcgc
     2041 cgcgcctgtc gttctttttt aacgctcaca acaatctcct ggaagaaatc gccaaatttc
     2101 gagccgcccg gcggctgtgg gctaaactga tgcgcgagcg tttcaaggcg cagaatccga
     2161 aatcactgat gctccgcttt cacacgcaga cggctggctc gaccctgacc gcgcagcagc
     2221 cggaagtcaa cgtcgtgcgc acgacgattc aggcgctcgc cgccgttctg ggcggaacgc
     2281 aaagcctgca taccaattcg atggacgaag ccttgtcgct gccgaccgaa aacgccgcgc
     2341 gcatcgctct gcggacgcag caggttatcg ctcacgaagc gggcgtcgcc gacacggtcg
     2401 acgcgctggc gggctcttac gcgatcgaag aactgaccaa tcgactggag caaaaggcgg
     2461 gcgaatacat cgaaaaaatc gacgcgatgg gcggaatgct gagggcaatc gagcagggct
     2521 acgtccagcg cgagattcag gaagccgctt atgaatatca acgcgcggtc gaaaaacaaa
     2581 cggcaatcgt cgtcggcgtc aacaagtttc agatggaaga gaccgaaacc attcccatcc
     2641 agcgcgtcaa tccgaaaatc gagcgcgaac aaatcgaaag gctgcaagcc gtccgcgcca
     2701 gccgcgacgc tgcgaaagcc gaaaacgctt tggcgaagct cgaagaatca gccaacggaa
     2761 cggaaaatct tttgccgcgc attctggact gcgtcgagag ccgcgtcacg gtcggcgaga
     2821 tttcgcaccg tttgagaaaa gtctggggcg aataccggga agccgtcacc gtttaaaaga
     2881 tgagacgatt aaaagccgtt acgaaggttt ggatttgagc ggtcaatatc gtcctgcgag
     2941 agtttattta gaagtttgcg agagcttatt taaaacagga agaagattgg cgcggcgcgg
     3001 cgcatcaagc cgcgcgcatc gaacaataaa tttatggctg aagaaagaaa cgtcgaagaa
     3061 ctggactcaa aaaacgaatc gaccgagctg cgctccatcc gcgacatcgt cagggattta
     3121 tccaagcccg tcgccaaaag gcatctgcgg acgcgcaagc agggcggcaa ggaattgaca
     3181 tatatctcgt ggtacgacgc cgtgaaatat ctcgaccatt acgcgccggg ctggtgttac
     3241 gaagtgcgct cgataaactc gatcagcggg aagctgattt taacgattcg gctttcgagt
     3301 ccctgccagg aaggcgttat ttaccgcgag gcaaccgggc aggaagaaga ggaaacgact
     3361 tcctacggcg attccagctc taacgccgaa tcgatggcgc tgcggcgggc ggcggcgaaa
     3421 ttcggtcttg gtttatatct ctacgaccag taattacagt aatgcagggt cacgccgccg
     3481 ggcgcgcgcc gcatttgtcc taaagactcg aaagccgctc cggattattt tcttttctcg
     3541 cgcgcctgtt gcggaaacgc cgccacgccc gcgagagtcc cgtc
//
LOCUS       DASWNE010000628         2113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_21840, whole genome shotgun sequence.
ACCESSION   DASWNE010000628 DASWNE010000000
VERSION     DASWNE010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21840"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..1607)
                     /locus_tag="VGB68_12280"
     CDS             complement(168..1607)
                     /locus_tag="VGB68_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050061.1"
                     /translation="MKQATFIMRRIVFPAIFFSVAIAAGLMYTAAALSQPRQEMRGDS
                     PLQSDEPVSESSRTAAITVPPQYRIYDIGLVQTGDTASQGFGVSTGGIAVGRSVRNGG
                     AQAFAWTLSGGIVGLPNLAGRAFCVSNGANNSGIVAGTCAASLFGTSRLPVIWQNGVA
                     SQLPLPGGETLGDANDVNASGVAVGSIGSGSLQRAVVYSGGNAAVITQTTSNGSFFLT
                     AFGVNDSGRVVGQGIDPTNAARNVGIVYDIGSTSAFEVAALPGANGALAFAVSNTGFV
                     VGSSMQNQGSGLPFIWTQPGGTTAIPLPSGTTQGSARAVNSAGWAVGTASSAFAVPFL
                     YDGAVTYRLADLIPAGTGWDLSMNTSSSALGISDNNVIVGTGVYNGQVRAYAMVPVTG
                     VSVGGRLLTAGGDGISNALVTLSGGNLPAPATVRTGGFGYYNFQGLQSGTTYTVTVST
                     KRYVFAQPTRMITPQSDVGNFDFVAEPQQ"
     gene            complement(1729..1872)
                     /locus_tag="VGB68_12285"
     CDS             complement(1729..1872)
                     /locus_tag="VGB68_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050062.1"
                     /translation="MASALVGTLKYGRTALASSPVSYFNIRRLARRQSLLSASAESPL
                     FQR"
BASE COUNT          486 a          592 c          600 g          435 t
ORIGIN      
        1 cctttgtata agaaaaggac tcattcgatt atgaatgagt cctttcgata aaacattgga
       61 gcttacgcgc aaattcgagc tcttttaatg tccagccgat tttgtaattc cgcgttccgt
      121 tatgacttcc agccggtgcg gaagacagcg tttgaccgct tcgctcgcta ctgctgaggt
      181 tccgccacaa agtcgaaatt accgacatcg gactgcggtg taatcatccg cgtcggctgt
      241 gcaaacacat atcttttggt cgagaccgtg acggtatatg tcgttcccga ctgtaatccc
      301 tgaaaattgt aataaccgaa acctcccgtt cgcacggttg ccggagccgg cagatttccg
      361 ccggacaaag tgaccagggc gttggagatt ccgtcgccgc ccgccgtcag cagacgcccg
      421 ccgaccgaga cgccggttac cggaaccatc gcgtaggcgc gaacctgtcc gttgtaaacg
      481 ccggttccga caatgacgtt attgtcactg attccgagag ccgacgacga agtattcatt
      541 gataaatccc agccggtgcc ggcgggaata agatccgcca gccggtaagt gacggcgccg
      601 tcgtagagaa acggaacggc gaatgcggaa gaggcggtac cgaccgccca acctgcggaa
      661 ttaaccgccc gcgccgaccc ttgcgtcgtg cccgaaggga gcgggattgc cgtcgttccg
      721 ccgggctgag tccagataaa cggcaagccc gaaccttgat tttgcatgct cgaaccgacg
      781 acaaaaccgg tgttgctgac ggcaaaagcc agagcgccgt tagcgccggg cagcgctgcg
      841 acctcgaagg ctgaagtgct gccgatgtca taaacgattc cgacattacg ggcggcattg
      901 gtcgggtcaa tcccctgacc gacgacgcgc ccggagtcgt tgacgccgaa tgcggtaaga
      961 aaaaaactcc cgttcgacgt tgtctgagtt atgacggctg cgtttccgcc gctgtaaacg
     1021 acggctcgct gcaaactgcc gctgccaatc gaaccgaccg cgacgcccga ggcgttcacg
     1081 tcgttggcgt cgccgagcgt ttcgccgccg ggaagcggaa gctgcgacgc gacgccgttt
     1141 tgccagataa caggcaaacg gctcgttccg aacagggaag ccgcgcaggt gcccgcgacg
     1201 atgccgctgt tattcgcgcc gttcgagacg caaaaggcgc gtcccgcaag attcggcagt
     1261 ccgacgatgc cgccgctaag agtccacgcg aatgcctgcg cgccgccgtt gcggaccgaa
     1321 cgcccgaccg cgatgccgcc ggtcgatacg ccgaaccctt gcgatgcggt atcgccggtc
     1381 tgcacaagtc cgatatcgta aattctatat tgcggcggga cggtaatcgc cgccgtccgg
     1441 ctcgattccg aaaccggctc atccgattgc aacggagaat cgcctctcat ttcctgacgc
     1501 ggctgagata atgcggcggc tgtatacatc aagccagccg ctatggcgac actaaagaaa
     1561 attgcgggaa agacgatgcg gcgcataata aatgttgctt gcttcatata tatttaattg
     1621 tgtaggactt tgacccagtc actttagagc gatttgccga caaagtcaaa atcgccgacg
     1681 agcgcgcctg gatgccgcag cccgcgctct ttgcgccgga aagtaagctc agcgctggaa
     1741 aagcggcgat tccgcagacg ccgataagag cgactgccgg cgagcgagcc ggcgtatatt
     1801 gaaataactg accggcgatg aggcgagcgc cgtgcgcccg tatttcaaag tgccgacgag
     1861 cgccgaagcc aaatcggcgt tttcggtcgg ttcggcgagc gctccggcac tcggtccgtc
     1921 gaatttgaat gatattgttg aaaaattccg ggtaaacttt tataccgaaa taattttggt
     1981 tcaatacaaa gttatgttgc atacagttga tgaaaaaaga agagggcgcg aaagaaacat
     2041 atgaagtctg gtttattaaa tgcgagatgg attctcatcc tcggtgtaat gcttcttttc
     2101 tcttgtcgga gct
//
LOCUS       DASWNE010000629         8007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_513826, whole genome shotgun sequence.
ACCESSION   DASWNE010000629 DASWNE010000000
VERSION     DASWNE010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8007
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513826"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1505)
                     /locus_tag="VGB68_12290"
     CDS             complement(156..1505)
                     /locus_tag="VGB68_12290"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0050063.1"
                     /translation="MKIISANYVLPISGAAIENGAVVIEKDKIVAAGKQAEIAAKFPE
                     AAAEDFGEAAIMPGFVNAHSHLEITAMRSFLDDVEHDFSRWLLKLSTVRAEKLTERDI
                     EISAVWGALEGLRAGVTCFGDIGRYGKAGFEALKKTGLRGIVFQETDFSPKNETAADD
                     FARLEEKFLALKKTETGLVKAGISPHSPYTVSRRLFEIIADYALREKVELSIHAAESK
                     EETEFMQRGTGFFAALYQKFGHEWHAPKVSSIEYFNQIGILQTAPLLAHCVEVSEKDI
                     ETIGESGTRIAHCPKSNAKFGHGIAPFEKFLDQRIKVGLGSDSVASNNTCDILEESRF
                     AALLARARQDKKRLLNAGEILEAATLGGAKSLNLEREIGTLDAGKQADLIVISLGAAA
                     QQPVHDVCSAILFASTARDVRLTMTGGEEIYRNGDAKTVDEDELRFEMKRIARQMVN"
     gene            complement(1636..2289)
                     /locus_tag="VGB68_12295"
     CDS             complement(1636..2289)
                     /locus_tag="VGB68_12295"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY0050064.1"
                     /translation="MKNRRRKVENGEPIIRFSLFDFRFPSKMRRYLLSGLVIFVVLSA
                     ALGLYFFNRYWIQRYDEIIEKQSGIYRLDKDLVWSVIYEETYFRAWQIGADEEVGLMQ
                     VTPTVAREWAKETGFREFERQTAENTVEFLRDPERNIQIGCWYLEKMREKYRDFPAQE
                     AMTLAAYNAGASRVEEWTRNAEISRLSEKEFIERINIASTKSYVTSILERYREKKTN"
     gene            complement(2290..3081)
                     /locus_tag="VGB68_12300"
     CDS             complement(2290..3081)
                     /locus_tag="VGB68_12300"
                     /inference="COORDINATES: protein motif:HMM:NF024399.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3881 family protein"
                     /protein_id="HEY0050065.1"
                     /translation="MEIAFDAIGLEIPNEKAFNNLAENAGKRGEASRLTRKTGVLHGR
                     CWKLGLGLEVWTVLYESQTGEVFYADCRPGFRARYIQRISPWIATEFDEEGEAVVHGF
                     IEDTEREVLFALQNLTEVGTNTSFQQNTLMVGLCGLAYRAEVLKNNAAPFWKSYNELA
                     LNVVENENDWSLCGEIISFSALRNPFSGSDLYWIYLNLGDFNIEILVNRRTLSGADLY
                     VGAMLRADIWLQGHVLNETARRSTYEGVDSSFRTIDFWKKLKKVN"
     gene            complement(3065..3247)
                     /locus_tag="VGB68_12305"
     CDS             complement(3065..3247)
                     /locus_tag="VGB68_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050066.1"
                     /translation="MLHEIREEMSREADYDMDLFAELVRSEEGKRKKEKGKNIKDDGQ
                     LTSDNRPLTTVYGNRF"
     gene            3488..3868
                     /locus_tag="VGB68_12310"
     CDS             3488..3868
                     /locus_tag="VGB68_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050067.1"
                     /translation="MNWDIEYIENRHYIRVKYEGLFSVTEQTAAIEDLLSRQFWQPGL
                     PILIDHRKLDFGNTNIPIIQQVGSFHQKNDARIGQGRIAVIMKSLSDFGRGRQFELIT
                     KGKISARVEIFLEEEKAINWLLKD"
     gene            complement(3948..4409)
                     /locus_tag="VGB68_12315"
     CDS             complement(3948..4409)
                     /locus_tag="VGB68_12315"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY0050068.1"
                     /translation="MKLSLYLETSVVGAYLDNGEPFRRDLTIRWWEHELSEYQPFVSP
                     LVRRELERVPEPHRTGYLKLIEPLKQLELTDEAAILAQGYISRGIFHRKYLADALHVA
                     LASFHKIDYLVTWNFGHLANVRKQARIRVFNVSAGFFVPMIVTPEFLIHSI"
     gene            complement(4406..6007)
                     /locus_tag="VGB68_12320"
     CDS             complement(4406..6007)
                     /locus_tag="VGB68_12320"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HEY0050069.1"
                     /translation="MRFEVLRKFDFPLPYRQAKIVCAGLVLIFFCASSRAAGDVRQTE
                     PENARQAVRQAEKLTRKGNLIEAEKILRRVVGQNPKDFKAKIKLAYVLMKRKRLLEAY
                     DYSLEVAKDEPQNAYAFAVLGTTLLNAGNFKQAYICFVNALKLDRGEALAWAGLGLLD
                     YYENRIERSLEYLKTAVYRDSQEPDYIFAVAQVSARAEKYRESADAYDRFLQVAPDTD
                     EERRNRIKGLITFLRFLGSKQTLYVAAGEEKTTVPVKLFRDRPVIEVKIGKHKEPLRF
                     VLDTGSGISVISEEVSRRLDIKPITTGGLARAIGGDGKFQIVYGFLRQIGIGEVKIQN
                     VPVYIRKFHENDEKIDGYIGLGLISKFLTTLDYGALTFSLVRQREVEATENNNPPSLP
                     LRLTSSGYLSGEVQLEGIEAPLNFIVDTGASVSVISDELAALTEVNKYISDEKMRVVG
                     AAGVTEGVSAFLLPRVTFGSHSREKIKAIALDLDIINETSGFEQAGILGGNFLKNYRL
                     TFDFKNSRVRFEPVASGVRNTLKPE"
     gene            complement(6038..6700)
                     /locus_tag="VGB68_12325"
     CDS             complement(6038..6700)
                     /locus_tag="VGB68_12325"
                     /inference="COORDINATES: protein motif:HMM:NF002305.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosylase/DNA lyase"
                     /protein_id="HEY0050070.1"
                     /translation="MKKEKEPVTIEKIRQTHAARREEIRARLAEFEAVWQSGTDERLW
                     EEMVFCFFTGGCSARMGMRSIEAVRPFLQNGDYVELSGALSGVHRYPFARAGYIVASR
                     DFLRQYCGLKLRDKLASFTDDLERRDWLVREKRIKGLGYKEASHFLRNIGLKGYAILD
                     KHVLRSLAELKIIDDPKPPNTRSKYLTVEQQLKNFACRLEIDFEEMDLVLWSLKTGEI
                     LK"
     gene            7036..>8007
                     /locus_tag="VGB68_12330"
     CDS             7036..>8007
                     /locus_tag="VGB68_12330"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEY0050071.1"
                     /translation="MFGSLKTKAFWLFIAMIALVGATTMTVSAQTTTQPYLQPIIVQP
                     TSTPLVKKTGGSTPVTPNPDISNPLDADINIPGYTGILVEDEKGRIVKESYSDYTFNP
                     ASNVKVATSYAVLKTFGPDYRFPTNVYYDGAIDKTTATLNGNLYISGRDPVFNLEHGV
                     AIADALNRLGVRSVTGDLVVTDKFIMGFTESTQRSGQSLLASLDAGKRGAGATRAWLD
                     FLVSSGKGNQTAYTPSVSVGGTLYVDIIPNTAKLLFSHESAPLREIVKVTNCYSNNFL
                     AERLGDMLGGAYAVARVVQMNANVAPQEFSLATSSGLGINRVTPKAMM"
BASE COUNT         1879 a         2151 c         1972 g         2005 t
ORIGIN      
        1 caagccgatg gccaattgct gcggaatctg tgggacgatc tgatcgagag gaggcattga
       61 ataaggtaaa aggcaaaagt aaaaaggcaa aaagtacttt gatagcgttt ttgccttttt
      121 acttttcctt tttacttttg ccttttattt ttgccttaat taaccatttg ccgcgcaatt
      181 cgcttcattt caaagcgcag ctcatcttca tcgaccgttt tcgcatcgcc gtttcgataa
      241 atttcctcgc cgccggtcat cgtcaggcga acgtcgcgcg cggtcgaagc gaacaaaatc
      301 gccgagcaga cgtcgtgaac gggctgctgc gccgccgcgc ctaatgagat gacgattaaa
      361 tcggcttgtt tgcccgcgtc gagcgtgccg atttcccgtt ccagattgag cgatttcgcg
      421 ccgccgagcg ttgccgcttc gagaatttct ccggcgttta gaagccgttt tttatcctgg
      481 cgcgcgcgcg ccagaagcgc cgcaaaacgc gattcttcca gaatgtcgca ggtgttgttg
      541 ctcgccaccg aatcgctgcc gagcccgact tttattcgtt ggtcgagaaa tttttcgaac
      601 ggcgcgatgc cgtgtccgaa tttggcgttt gatttcgggc aatgggcgat gcgcgtgccg
      661 ctttcgccaa tcgtctcgat atctttttcc gaaacctcga cgcagtgcgc gagcagcggc
      721 gcggtctgca aaatgccgat ttggttaaaa tattcgattg aagaaacttt cggcgcgtgc
      781 cattcgtgac cgaatttttg ataaagcgcg gcgaaaaagc ctgttccgcg ctgcataaat
      841 tcggtttctt ccttcgattc ggcggcgtga atcgaaagct cgaccttctc gcgcaaagcg
      901 taatcggcga tgatttcaaa aagacgacgg ctgaccgtgt acggcgagtg cggcgagatt
      961 ccggctttga ctaaaccggt ctcggttttc tttaaagcca gaaatttttc ttcgagccgg
     1021 gcaaaatcgt cggcggcggt ttcgtttttc ggcgaaaaat cggtttcctg aaagacgatt
     1081 ccgcgcagtc cggttttctt taacgcttca aaacccgctt tgccgtagcg cccgatgtcg
     1141 ccgaaacagg tgacgcccgc gcgcaagcct tcgagcgcgc cccagacggc ggaaatttca
     1201 atatcccgtt cggttaattt ttcggcgcga acggtcgaaa gcttcaaaag ccatctggaa
     1261 aaatcgtgct cgacatcatc gagaaagctg cgcatcgccg tgatttccag atgcgaatga
     1321 gcgttgacga aacccggcat tatcgccgct tcgccgaaat cttccgccgc cgcttcggga
     1381 aattttgcgg cgatttccgc ttgtttgccg gcggcgacga ttttatcttt ttcaataacg
     1441 accgcgccgt tttcaatcgc agcgccggaa atcggcagaa cgtaattggc ggagatgatt
     1501 ttcatagctt ctcgacaaga agaaacgacc gcgccgattt taagattatt taagattgat
     1561 taagatttta ttcgaattta tcctaattaa tcttaaataa tcctatcaaa cgggacgtcc
     1621 cgtatttctt ccgcgctaat tcgttttctt ttcgcggtat ctctccagaa tcgaagtcac
     1681 gtaggatttc gtcgaggcga tattgatgcg ctcgataaat tccttttccg acaggcgcga
     1741 gatttccgcg ttgcgcgtcc attcttcgac gcggctggcg cccgcgttat atgccgccag
     1801 cgtcatcgcc tcctgagcgg gaaaatcgcg gtatttttcg cgcatttttt ccagatacca
     1861 gcagccgatt tggatattac gctccgggtc gcgcaaaaat tcgaccgtat tttccgccgt
     1921 ctgccgttca aattcgcgaa agcccgtctc tttcgcccac tcgcgcgcca ccgtcggcgt
     1981 cacctgcatc agcccgactt cttcgtcggc gccgatttgc cacgcccgaa aatatgtttc
     2041 ctcgtagatg acgctccaga ccaaatcttt atccagacga taaatgcccg attgtttctc
     2101 gatgatttca tcgtagcgtt gaatccagta gcgattgaaa aaataaagtc ccagagcggc
     2161 ggaaagaacg acgaaaatga ccagaccgga gagcagataa cgacgcattt ttgaaggaaa
     2221 acgaaaatcg aaaagcgaaa agcggataat tggctctcca ttttccactt tccgtcttct
     2281 atttttcatt taatttactt ttttgagttt cttccaaaaa tcaattgttc tgaacgaaga
     2341 atcgacgcct tcataagtcg agcgccgcgc cgtttcgttc aaaacgtgac cttgcagcca
     2401 gatatccgcc cgcagcatcg cgccgacgta taaatccgcg ccggacaagg ttcggcgatt
     2461 gaccagaatt tcgatattga aatcgcccag attcagataa atccagtaaa ggtcgctgcc
     2521 cgaaaacggg ttgcgcagag cgctgaaaga aataatctcg ccgcacagac tccaatcgtt
     2581 ttcgttttcc acgacgttta aggcaagctc gttgtaagac ttccagaacg gcgcggcatt
     2641 attttttaag acctcggcgc gatacgccag accgcacaaa ccgaccatta aagtattttg
     2701 ctgaaagctg gtgttggttc cgacttccgt cagattttgc agcgcaaaca aaacttccct
     2761 ttcggtgtct tcgataaaac cgtgcacgac cgcctcgcct tcctcgtcaa attcggtcgc
     2821 aatccacggg ctgatgcgct ggatgtagcg ggcgcgaaaa cccgggcggc agtcggcgta
     2881 aaaaacttcg cccgtctgcg attcatacaa aaccgtccac acttccagac ccagaccgag
     2941 cttccagcag cgcccgtgaa gcacgccggt tttgcgcgtc aggcgcgagg cttcgccgcg
     3001 ttttccggcg ttttccgcta aattattgaa agccttttcg tttggaattt ccaaaccgat
     3061 ggcatcaaaa gcgatttcca taaacagtcg tcagcggtcg gttgtcgctg gtcagttgcc
     3121 cgtcgtcctt tatgtttttg cctttttcct tttttctttt gccttcttcg cttctgacca
     3181 attcggcaaa caaatccata tcgtaatcag cctcgcgcga catttcttcg cggatttcgt
     3241 ggagcacttc taaaaatttc ggtcgtcgat acattacaaa gtgaatagta aatctttaaa
     3301 gggcaatggt caataggttc gcaaacgcgg caacgtccta atatttatgc ggcggaaaat
     3361 aattgctttt cagtatctca aaaaagcctc tcgccgccgc gccgaaagtc atacgccttg
     3421 gagacggatt tcggatttaa actttagctt attattgttt aaaattagcg tcgagaataa
     3481 aagctttatg aattgggaca ttgaatacat cgaaaatcgg cattatatca gagtcaagta
     3541 tgaaggctta ttttccgtca ccgaacagac agcggcgatt gaagatttgc tttcgcgcca
     3601 attttggcag cccgggctgc ctattctaat cgaccaccga aaactggact tcggcaatac
     3661 aaatatcccg attatccagc aggtcggcag ttttcatcaa aaaaacgacg cccgcatcgg
     3721 gcagggacga atcgccgtca ttatgaaatc gctttcggat ttcggtcgcg gacgccagtt
     3781 tgaattgatt accaaaggaa agatttccgc aagagtagaa atctttcttg aggaagaaaa
     3841 agccatcaat tggctgctca aagactaaac cgaacggcat tttcgagagg aaactatttt
     3901 gatttgttcc gccgctccgc agctggcgcg tttcttccgg cggcggctca aatgctgtga
     3961 atcagaaatt ccggcgtcac aatcatcggc acgaaaaatc ccgccgaaac gttaaaaacg
     4021 cggatgcgcg cctgtttgcg gacgtttgcc agatgcccga aattccacgt tacgagataa
     4081 tcgattttgt gaaacgaagc cagcgcgacg tgcagcgcgt ccgccaggta tttgcgatga
     4141 aagatgccgc gcgagatgta gccctgcgcc agaattgcgg cttcgtcggt cagttccagc
     4201 tgtttgagcg gttcgatgag ctttaaatag cccgttcggt gcggttcggg aacgcgttcg
     4261 agctcgcggc gcaccagcgg cgagacaaac ggctgatact ccgaaagctc gtgctcccac
     4321 cagcgaatgg tcaaatccct tcgaaacggc tcgccgttgt cgaggtaagc gccgacgacc
     4381 gatgtttcca gatataaact cagcttcatt cgggtttcag agtgttgcgg acgccggaag
     4441 cgaccggctc aaatctcacg cgcgaatttt taaaatcgaa cgtcagacga taatttttca
     4501 gaaaattacc gcccagtatt cccgcctgct cgaagccgga agtttcgttg ataatgtcca
     4561 aatccagcgc gatggctttg attttttcgc ggctgtgact gccgaacgtg acgcgcggca
     4621 gcagaaacgc cgaaacgccc tcggtgacgc ccgccgcgcc gacgacgcgc attttctcgt
     4681 ccgaaatata tttattaacc tcggtcaaag ccgccagctc gtccgagata acggagacgc
     4741 tcgcgcccgt atcgacgata aaattcagcg gcgcttcgat gccttcgagc tgaacttcgc
     4801 cgctcagata accgctcgac gtcagcctca gcggcagcga cggcggatta ttgttttccg
     4861 tcgcttcgac ctcgcgctgc cgcacgagcg aaaaggtcag cgcgccgtaa tcgagcgtgg
     4921 tcaaaaattt cgagatcagc ccgagcccga tgtagccgtc gattttctcg tcgttttcgt
     4981 gaaattttct gatatagacc ggcacgtttt gaatttttac ttcgccgatg ccgatttggc
     5041 gcaaaaatcc gtaaacgatt tgaaacttcc cgtcgccgcc gatggcgcgc gccagcccgc
     5101 ccgtggtaat cggttttata tccagacgcc gcgagacttc ttcgctgatg acggaaattc
     5161 ccgaaccggt atccagcaca aaccggagcg gctctttgtg ttttccgatt ttcacctcga
     5221 tgaccgggcg gtcacgaaac agtttgaccg gaacagtggt tttctcttct ccggcggcga
     5281 catacaaagt ctgtttgctg ccgagaaaac gcagaaaagt aatcaagcct ttgatgcgat
     5341 tgcggcgctc ctcgtcggtg tccggcgcaa cctgcagaaa acggtcgtag gcgtcggcgg
     5401 attccctgta tttttcggcg cgggcggaga cctgcgcgac ggcgaaaatg taatcgggct
     5461 cctgcgagtc gcgatacacg gcggttttta aatactccag acttctctca atccggtttt
     5521 cgtaataatc gagcagtccg agcccagccc acgccagcgc ttcgcctcta tcgagcttca
     5581 gcgcgttaac aaaacaaatg taggcttgct tgaaatttcc ggcattgagc aaagtcgtgc
     5641 ccaaaacggc gaaagcgtat gcgttctgcg gctcgtcctt cgccacttcg agcgaataat
     5701 cataagcttc gagcaggcgt tttcgcttca tcaaaacgta tgcgagcttg attttggctt
     5761 tgaaatcttt cggattttgc ccgacgacgc ggcgcagaat tttttcggct tcgattaaat
     5821 ttcccttgcg ggtcagcttt tccgcctgcc ggacggcttg gcgcgcgttt tccggctcgg
     5881 tctgccggac atcgcccgcc gcccgcgacg acgcgcagaa aaatatcaaa accaacccgg
     5941 cgcaaacgat ttttgcctgg cggtacggca gcggaaaatc aaactttcgc agaacttcaa
     6001 atctcatgac gaaaactcct tttccccgga ttaaatttta cttgaggatt tcgcccgttt
     6061 tcagcgacca caaaaccaaa tccatctctt cgaaatcgat ttccaatcgg caggcaaaat
     6121 tttttaactg ttgttcaacg gttagatact ttgaacgcgt atttggtggc ttcgggtcgt
     6181 caataatttt cagctccgcc aagctgcgca aaacgtgctt gtccagaatg gcgtagccct
     6241 tcagcccgat gttgcgcaaa aagtggctcg cttccttgta gcccaagcct ttgatgcgtt
     6301 tttccctgac cagccagtcg cggcgctcca ggtcatccgt aaagctcgcc agcttatcac
     6361 gcagctttaa accgcagtat tggcgcagaa aatcgcggga ggcgacgatg tagccggcgc
     6421 gcgcaaacgg gtagcggtga acgccggaaa gagcgccgga aagctccacg tagtcgccgt
     6481 tctgcaaaaa cggacgcacc gcttcgatcg agcgcattcc cattcgcgcc gagcagccgc
     6541 ccgtaaaaaa acaaaaaacc atctcttccc acaggcgctc gtccgtgccg gattgccaga
     6601 cggcttcgaa ctcagccagg cgcgcgcgaa tttcttcgcg gcgggcggcg tgagtttgtc
     6661 tgattttttc aatggtgacg ggttcttttt cttttttcac aaaaatcttt tcgtagttta
     6721 tgaaccggcg cgagagattg tcaatgtttt ttgcaaaatt ccgtttccgc ggaaaatttc
     6781 ttcgaaaaat cttttctttt cgtcgttttt aatatataag taaacggaat cgtatgcata
     6841 agccattctg aaaagctttg catccgaatc gggcgaattg acatcaaatc gagcgtactg
     6901 caaaaaagcc gcgaaaggtt tcggattttt ttgcttgata aggaatatag gtgctgtggt
     6961 ataatccaaa tcagccttcc ttttcgcgta agggaaagcc tgccacgaca caaaaaaaca
     7021 cggagtaaaa tatagatgtt cggttcctta aagactaaag ccttttggtt gtttatcgct
     7081 atgattgcgc tcgtcggcgc aacgacaatg accgtttcgg cacaaaccac gacgcagcct
     7141 tatctgcagc cgattatcgt gcagccgact tcgacgccat tagtgaaaaa aaccggcggc
     7201 tcgacgcccg tcacgccaaa cccggacatt tcgaatccgc tggacgccga catcaacatt
     7261 ccgggctata caggcattct ggtcgaagac gagaaaggca gaatcgtcaa ggaaagctat
     7321 tcggattaca cgtttaatcc ggcttccaac gtcaaggtcg ccacttctta cgcggtgctg
     7381 aaaaccttcg gtcccgatta tcgttttccg accaatgttt actacgacgg cgcgatagac
     7441 aaaacaaccg cgacgcttaa cggcaatctt tatatttcgg gacgcgaccc ggtatttaat
     7501 ctcgaacacg gcgtggcgat tgccgatgcg ctgaaccggc tcggcgtgcg cagcgtcacg
     7561 ggcgatttgg tcgtcaccga caaatttatt atgggcttta ccgaatcgac gcagcgttcg
     7621 ggacaatccc tgctggcttc gctcgatgcg ggcaaacgag gcgccggagc gacgcgcgcg
     7681 tggcttgatt ttctggtttc ttcgggaaaa ggcaatcaaa ccgcttatac gccgagcgtc
     7741 agcgtcggcg gcacgcttta tgtcgacatt attccgaaca ccgccaagct tctcttctcg
     7801 cacgaatcgg cgccgctgcg cgaaatcgtc aaagtcacca attgctattc aaacaacttt
     7861 ttagccgaac gcctcggcga tatgctcggc ggcgcttatg ccgtcgcgcg cgtcgtgcag
     7921 atgaacgcca acgtcgcgcc gcaggaattt tcgctggcga cttcgagcgg tctcggaatt
     7981 aaccgcgtca cgccgaaagc gatgatg
//
LOCUS       DASWNE010000630         5963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_232617, whole genome shotgun sequence.
ACCESSION   DASWNE010000630 DASWNE010000000
VERSION     DASWNE010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5963
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232617"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /gene="ybeY"
                     /locus_tag="VGB68_12335"
     CDS             <1..193
                     /gene="ybeY"
                     /locus_tag="VGB68_12335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEY0050072.1"
                     /translation="FLGDIVISLEQAQRQAAENNLTLENEIKQLILHGILHLCGYDHE
                     SDGGEMNRLELKLRRKLKV"
     gene            298..1611
                     /locus_tag="VGB68_12340"
     CDS             298..1611
                     /locus_tag="VGB68_12340"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEY0050073.1"
                     /translation="MEIEIAVAVLILFALVFLATIDMAFTQLSDVSLRRLSTDTEEKP
                     QSRSTLFLREILENRPRFRFALSAAIQILLIIFSVLVTLIVFNFSQSSGALLFYSLLI
                     GLILSGIFRQFIPRLLTLNNPEGKLLMLLPLVRPLYSVMSFLADPFDGFMRDKEQQNL
                     EITITPSATEERDEDNNENIQALIEVGEAEGIIEERERELIETMFEFSETRAGEIMTP
                     RTEICALPINATVKQARDLIIEEKYSRIPVYRDRIENIEGVIYVRDLLIAWSEGKENE
                     TIENLLRKPLFVPETKPADELLKIMQSSHIQLAVVIDEYGGVAGLVTVEDILEEIVGE
                     IEDEDTEQEEIIEIIEGEDGYFDVLGSTEIHKIERLFDMEIEDEDFTTIAGLVTSEVG
                     YVPKPHEKLALHGLSVEILRADEKRINLLRLRKATEPEDGEDEIS"
     gene            1939..5202
                     /locus_tag="VGB68_12345"
     CDS             1939..5202
                     /locus_tag="VGB68_12345"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0050074.1"
                     /translation="MSDKTLEYLTENDVRLSSVINQITVGIVECDLAGRLVMVNDKFC
                     EIIGFRREELLGKHISELTAPEDLPQSLNLFQKCAAEGEPFTVEKRYARKDGAEVWVK
                     NSVSLLRDAEGKPRRVMGVVVDFTAEKLAQTALLQSEQRYRALIDASAQMVLTMKADG
                     SRDLHRQWFSRLTGVPVEELQGNKYLDVIHPDDRPNLRKVMSQLTQNPAPYEVEVRLR
                     PKSGENYRFYSLRGVPVFKADGTLNEWIGTVSDITDRKEAEMARRHIDKTKAHLAAII
                     ESSDDAIISKDLSSIITSWNRGAEKVFGYTAEEVIGKSVTVLMPPNLIDEESQILKRI
                     KRGESIEHYETRRRRKDGTDIDISLTVSPIRDDSGNVIGASKIARDITERKESEEALR
                     ESQAILSLSMRSSRMGAWVQDIKTDYIWWSQELEEIFGLEKGSFSSNRKGFYEFVHPD
                     DRERILDEVRQAISEQKDYTIEFRFLHTDGSVRWMEGRGQAFYSEKGEVVRLYGIGID
                     ITERRQIQEQLIRSERRAAEDYQALLARIVPLAQTLGTARDLISIYRSVGEFVRTSTP
                     CNAFFVSFYDAKNNLRTAAYVSGKEGEVDVSTLPPMLLTEDGGPNSQAVFQKKSVIVN
                     RYMDLMKDRPHVVLQEDGIEPMSSLVVPMTVMNRITGTLEVQAYENGAFTQEHVVALE
                     MVANLAAVAIENVRLLQVEERARQEAETANRAKDEFLSVLSHELRTPLNSMFGWTRML
                     RSGILDEDKTSQAIEVIERNVRLQNSLIEDMLDVSRIITGKMRIEKEEIDFAAIVKSA
                     VETARPQAEQKKVSLVFNADAESYVLLGDEVRLQQIVNNLINNAVKFTPEDGAVILSL
                     STAADDSVLLNVTDTGIGIEPDFLPQIFDRFRQADSTTRRTHSGLGLGLTIVRHLIEL
                     HGGRVSAESEGIGKGASFTIELPLVSKPQADLLKNAAAPGDGFSLKGSRILLVDDDCD
                     AMVPLQIILEAREAEVLCASAVSEALEKLAADKFNLLVSDIGMPEMDGYDLITALRKM
                     DESSNHDIPAIALTAYASTQDKRRALMSGFQHHFAKPLDFEQFLATVCSLIKHSENK"
     gene            5602..5874
                     /locus_tag="VGB68_12350"
     CDS             5602..5874
                     /locus_tag="VGB68_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050075.1"
                     /translation="MSPKQIEQINRWEKERADGKWFWIFKRASAWLVSVILMFGAANY
                     FAAETISFGGSQIAVAAFMFGGFFIGSIMEWAKMEELYETTSLTTD"
BASE COUNT         1572 a         1516 c         1561 g         1314 t
ORIGIN      
        1 ttttctcggc gacatcgtta tcagcctcga gcaggcgcag agacaagccg cagaaaacaa
       61 tctgacgctc gaaaacgaaa tcaagcaatt aatcctgcac ggcatcctgc acctgtgcgg
      121 atacgaccac gaatcggacg gcggcgaaat gaatcgactg gagttaaaac tcagacggaa
      181 acttaaggta taatgtttcc ttgtcggctc agtaaaattt agccgcagat ttgagcgtta
      241 tgcagattaa acgaatatgc aactgactac cggcgacaga caactaatca ctcttttatg
      301 gaaattgaga tagccgttgc cgttttaatc ctcttcgcgc tggtttttct ggcgacgatt
      361 gatatggcat tcacgcagtt gtcggacgtt tccctgcgcc gtctctcgac cgacacggaa
      421 gaaaaaccgc aatcgcgctc gacgcttttt ctgcgcgaga ttctggaaaa tcgcccgcgt
      481 tttcgctttg cgctgtccgc ggcgattcag attctgctga ttattttttc ggttctcgtc
      541 acgctgattg tctttaattt ttctcaaagc tccggcgcgc tcctgtttta ctctctcctt
      601 atcggcttga ttttatcggg cattttcagg cagtttatcc cgcgcctgct gactttgaat
      661 aatccggaag gcaagcttct gatgctgctt ccgctggtgc gcccgcttta cagcgtgatg
      721 tcttttctag ccgacccgtt cgacggtttt atgcgcgata aggaacagca aaacctcgaa
      781 atcacaatta cgccgagcgc gaccgaggaa agagacgagg acaacaacga aaacattcag
      841 gcgcttatcg aagtcggcga ggcggaaggc attatcgagg aacgcgagcg cgaactgatt
      901 gaaacgatgt tcgagttttc ggaaacgcgg gcgggcgaga ttatgacgcc gcgcacggag
      961 atttgcgcgc tgccgataaa cgcgaccgtc aaacaggcgc gcgatttgat tatcgaggaa
     1021 aaatattcgc gcattccggt ttaccgcgac cgcatcgaga acatcgaagg cgtgatttac
     1081 gtccgcgatc tgctgatagc gtggtcggaa ggcaaggaaa acgaaaccat cgaaaatctg
     1141 ctgcgcaaac cgctgttcgt gcccgagacc aaacccgccg acgagctttt aaaaattatg
     1201 cagagcagtc acatccagct cgccgtcgtg attgacgaat acggcggcgt cgccggtctg
     1261 gtgacggtcg aagacattct ggaagaaatc gtcggcgaaa tagaggacga agacaccgag
     1321 caggaagaaa tcatcgagat tatcgagggc gaagacggct atttcgacgt gctcggctcg
     1381 accgagattc acaaaatcga gcgtcttttc gatatggaaa tcgaggatga agattttacg
     1441 accattgccg ggctggtgac gagcgaggtc ggctacgttc ccaaaccgca cgaaaagctc
     1501 gcgctgcacg gtttgagcgt cgaaatcctg cgcgccgacg agaaacgaat caatctgctg
     1561 cgcctgcgca aagcgaccga gccggaagac ggcgaagacg aaataagtta attgataact
     1621 gaaaaccggg aaccgagaac tgaaatcatt tcttgctttt tagttatcaa ccatcagtta
     1681 tcagttccaa ttgtttactt ttgcagagcg attggttaaa atgagcgggt aaaactcttt
     1741 tacaaaatta cctgaaataa aatcctgaaa atttcatata cttccctttt taatctttca
     1801 ggcagcagtt tatcgcggaa ttaattccat aattcggttc aggcagtaaa aacaaatatt
     1861 tatcaggctt cgagtctgaa gaagcggttt ggttgactgg ttttagccat tttgtcagcc
     1921 cagcgaaatt tgcagaaaat gtcggacaaa acattagaat atttaaccga aaacgacgtg
     1981 cggctcagct ccgtcattaa tcagataacc gtcggcatcg tcgagtgcga cctggcggga
     2041 cgactggtta tggtcaacga taaattctgc gaaatcatcg gcttccggcg cgaagaactg
     2101 ctcggaaagc acatctccga gctgaccgcg ccggaggatt tgccgcagag tctgaatctt
     2161 tttcaaaaat gcgcggctga aggcgagccg ttcaccgtcg aaaaacgcta cgcgcgcaaa
     2221 gacggcgctg aagtctgggt caaaaacagc gtgtcgcttc tgcgcgacgc cgaaggcaag
     2281 ccgcgacgcg tgatgggcgt cgtcgtcgac tttaccgccg aaaaactcgc tcaaaccgct
     2341 ctgcttcagt ccgaacagcg ttatcgagcc ttaatcgacg cttccgcgca gatggttttg
     2401 acgatgaaag ccgacggcag ccgcgatttg catcgtcaat ggttttccag attgaccggc
     2461 gttccggtcg aagaattgca gggaaataaa tatctcgacg tcattcaccc ggacgaccgc
     2521 ccgaatctgc gaaaggtgat gtctcagctg acgcagaatc cagcgcctta cgaagtcgaa
     2581 gtgcggctgc gcccgaaaag cggcgaaaac tatcgctttt acagtctgcg cggcgttccc
     2641 gtttttaaag ccgacggcac tttaaacgaa tggatcggca cggtcagcga cattaccgac
     2701 cgcaaggaag cggaaatggc gcgccgtcat atcgataaga caaaggctca tctggcggct
     2761 attatcgaat cgtccgacga cgccattatc agcaaggatt taagcagcat tattacgagc
     2821 tggaatagag gcgcggaaaa agtattcggc tacacggcgg aagaagtaat cggaaaatcg
     2881 gtgacggtac tgatgccgcc gaacctcatc gacgaagaat cgcaaatcct caaacgaatc
     2941 aagcgcggcg aaagcatcga acattatgag acgcgccgcc gccgcaaaga cggaaccgac
     3001 atcgatattt cgctgaccgt atcgccgatt cgcgacgatt cgggcaacgt catcggcgcg
     3061 tcgaaaatcg cgcgcgacat caccgagcgc aaagaatccg aagaagccct gcgcgaaagc
     3121 caggcgattt tgtcgctgtc gatgcggagc agccggatgg gcgcttgggt gcaggacatt
     3181 aaaaccgatt acatctggtg gagtcaggag ctggaagaaa ttttcgggct ggaaaagggc
     3241 agcttttcca gcaaccggaa aggattttac gaattcgtcc atccggacga ccgcgagaga
     3301 attctcgacg aagtccggca agccatcagc gagcagaaag attacactat cgaatttcgc
     3361 tttctgcaca ccgacggcag cgtgcgctgg atggaaggac gcgggcaggc tttttattcg
     3421 gaaaagggcg aggtcgtcag gctttacggc atcggcatcg atataaccga gcgcagacag
     3481 attcaggaac agcttatcag aagcgagcgc cgcgcggcgg aagattacca ggcgctgctg
     3541 gcgcgcatcg taccgctggc gcagacgctc ggcacggcgc gcgatttgat ttccatttac
     3601 cgctcggtcg gcgagttcgt ccgcacctcg acgccgtgca acgcgttttt cgtttcgttt
     3661 tatgacgcga agaacaactt gcgaaccgcc gcatacgtct ccggcaaaga aggcgaagtc
     3721 gatgtttcga cgctgccgcc gatgcttctg accgaagacg gcggacccaa ttcgcaagcc
     3781 gtctttcaaa aaaaatccgt tatcgtcaat cgatatatgg acttgatgaa agaccgcccg
     3841 cacgtcgtcc tgcaggaaga cggcatcgaa ccgatgtcgt cgctggtcgt gccgatgacg
     3901 gtgatgaacc gcattaccgg cacgctcgaa gttcaggcgt acgaaaacgg cgcttttacg
     3961 caagagcacg tcgtcgcgct ggaaatggtc gccaatctcg ccgccgtcgc catcgaaaac
     4021 gtgcggcttt tgcaggtcga agaacgcgcc cgacaggaag ccgaaaccgc caatcgcgcc
     4081 aaggacgaat ttctttcggt tctctcgcac gagctgcgca cgcctttgaa ctcgatgttc
     4141 ggctggacgc ggatgcttcg ctccggcatt ttggacgaag acaaaaccag ccaggcgatc
     4201 gaggtcatcg agcgcaatgt ccgcctgcaa aacagtctta tagaagatat gctcgacgtc
     4261 tcgcgcatca tcaccggcaa aatgcggatt gaaaaagagg agatagattt tgccgcaatc
     4321 gtcaaatcgg cggtcgaaac cgcccgcccg caggctgaac agaaaaaagt ctcgctcgtc
     4381 tttaacgccg acgccgaatc atacgttttg ctcggcgacg aagtcagact tcagcaaatc
     4441 gtcaacaatc tgattaacaa tgccgttaaa ttcacgcccg aagacggcgc ggtcatttta
     4501 agtttatcga ccgccgccga cgattccgtt ttattaaacg tcacggacac gggaatcggc
     4561 atcgagccgg attttctgcc gcagattttc gaccgtttcc ggcaagccga cagcaccacg
     4621 cggcgcactc acagcgggct cgggctcgga ttgaccatcg tccggcattt aatcgagctt
     4681 cacggcggac gcgtcagcgc cgagagcgaa ggaatcggaa aaggcgcgag ctttaccatc
     4741 gagctgccgc tggtatcgaa accgcaagcc gatttactta aaaacgccgc cgcgcccgga
     4801 gacggatttt cgctgaaagg ctcgcggatt ctgctggtcg acgacgattg cgacgcgatg
     4861 gttccgctgc agattattct ggaagcgcgc gaagccgaag ttttatgcgc ttcggcggta
     4921 agcgaggcgc tcgaaaaact tgccgccgat aaattcaatc tgctggtcag cgatatcgga
     4981 atgccggaaa tggacggtta cgatttgata accgcgctgc gaaagatgga cgaatcgtca
     5041 aatcacgata ttccggcaat cgccctgacg gcttatgctt cgacgcagga taaacggcgc
     5101 gccctgatga gcggttttca acatcacttt gccaaaccgc tcgattttga acaattttta
     5161 gcgaccgtct gctcgctgat taagcattca gaaaataaat gattcaagat tcaggattca
     5221 ggatttagga ttcaggattc aggattcagg attacaaatc ttgaatcttg aatcttgaat
     5281 cttgaatctt gaatcttaaa tcctgaatcc tgaatcctga atcccaaatc tgcttaaaaa
     5341 tggaaaattc agaccaatca aacgaaataa tctgcgaatc gtgcggcgaa aatttcgcct
     5401 gcggcgcaaa atctgacaaa tgttggtgtt tcgggcttga aataaacggt gaaactctgg
     5461 caaaattaaa aaaggatttt cgccggtgtc tctgccggcg ctgtctggaa aaaatatcaa
     5521 ccacctagct tgctcaaaca agaaaggcaa taactacaaa tgaactttaa atttaccaac
     5581 ccgcggaagg ttggaaaatc gttgtctccg aaacaaatcg aacagattaa ccgttgggaa
     5641 aaagaacgcg ccgacggcaa atggttctgg attttcaaac gcgcttcggc gtggctcgtc
     5701 agcgtgattc tgatgttcgg cgcggcaaat tattttgcgg ctgaaaccat cagtttcggc
     5761 ggcagtcaga ttgcggtcgc ggcttttatg ttcggaggtt tctttatcgg ctcgataatg
     5821 gaatgggcga agatggaaga gctgtacgaa accacttcgc tgaccaccga ctagacaaag
     5881 attttcagcc tgcccgatac aacaaagaca atttaccttt caaacacacg aaatcttgcg
     5941 caaacggatt ttgataatcg agt
//
LOCUS       DASWNE010000631         3847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_552491, whole genome shotgun sequence.
ACCESSION   DASWNE010000631 DASWNE010000000
VERSION     DASWNE010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552491"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /gene="kduI"
                     /locus_tag="VGB68_12355"
     CDS             <1..806
                     /gene="kduI"
                     /locus_tag="VGB68_12355"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005886840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HEY0050076.1"
                     /translation="GADNEALRELYLIGDLFAAGELRLNYTHFERMVVGGAFPVGNSL
                     ALPRQTEPASAKDKPFLERREMGAVNVGAGKGIVSVDGARFELAAKDCLYVPMGSAEV
                     SFESSEPENPAKFYLVSTPAHARFQTRHISAEEANKLHLGAAETSNVRTVYQFIIPGI
                     CQSSQLVLGLTSLQTGSVWNTCPPHIHERRSEVYLYFDLDENSRIMHFMGQPEDMRHI
                     VLANEEAVVSPPWSIHMGAGTSNYSFVWAMGGENLDYTDMNVLDICQLK"
     gene            complement(968..3118)
                     /locus_tag="VGB68_12360"
     CDS             complement(968..3118)
                     /locus_tag="VGB68_12360"
                     /inference="COORDINATES: protein motif:HMM:NF027484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HEY0050077.1"
                     /translation="MKHKSLILALICFAFVPGIWLGGIFDKSATVSAQVAPPPEEIEI
                     LSTQSIVMNVTVSGANVGAQGGYTVPRVTLLPGDMVTIDPTGGTVDFNTSGICTGSAY
                     DASPGGKPRSEIYQGSCWDEDLNYCQINQNDPLPAQNHAGLFRPSQPGIGSIKWLGNN
                     NDAFTYTGTTARDLRLGVNDAACPSSPNGGAFNVKVTIKRPQVQTLNVVVRGTNTASA
                     EVFGAPLAAGDQVVIDNISGTVDFLTDAVAVGCPHVVNANGLARELFENPQNQLLCDY
                     QLDNPADPMTGPSHGHAGLYMKRNTTRTFIGRNGASFTALGAETLRFGVNDVRGNNNS
                     GQFNARVTITHPSSKQNVVVEADSIAGNEEFVANLQPGDDVLITNVTGTVTFETPLQS
                     CVNSGLIYTVNAGGLNRGVYETPGNLPCYGNDRTVGADPLTAPAHGHAGLFMKRGGSR
                     FFIGTNGASFTATAAQSLWLGINDSYAANNSGRFRADVFITRAGAFRFSAATYAVDES
                     GASAVVNVTRTVSTGAASVNYATSNGTALAGQDYTARSGVLYFADGETTKSFSIPIIN
                     DAADEPNETVNLTLSNPVGGSILVKPFTAVLTIDDNDAAGNNSAFVSQMVPSQMSAGQ
                     SYTVSVTMRNTGGTTWTPGIYKLGSQNPRDNWTWGTNRIQLENPVAPNETETFVFIVT
                     APAAPGTYNFQWKMVQEGVQWFGATAPNVIVSVR"
     gene            3397..>3847
                     /locus_tag="VGB68_12365"
     CDS             3397..>3847
                     /locus_tag="VGB68_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050078.1"
                     /translation="MRKFSIILIIICAVNFVFAQTGGEVLEFKSKIGFTGGEDKIVEY
                     KFLENGRKLLLIGEKNLQIWDVENARLLNAATHQIPQFAPRGFFSTYLLMGVPRLLDW
                     RPFLVDEGGRWIITVEKIGANPLRSAVVRDLQNLKQIAALDLPNVSTD"
BASE COUNT          873 a         1013 c         1067 g          894 t
ORIGIN      
        1 acggcgcaga caatgaggcg ctgcgcgagc tgtatttaat cggcgatttg tttgccgccg
       61 gcgagcttcg actcaattac acgcattttg agcgaatggt cgtcggcggc gcgtttcccg
      121 tcggcaattc tctcgcgctt ccgcgacaga ccgagcccgc ctcggcgaaa gataagcctt
      181 ttctggaaag gcgcgagatg ggcgccgtca atgtcggcgc aggcaaagga atcgtcagcg
      241 tcgacggcgc gcgattcgag cttgccgcca aagactgtct gtatgttccg atgggcagcg
      301 cggaggtttc gttcgaatct tcggagccgg aaaatccggc gaaattttat ctcgtttcga
      361 ctccggctca cgcgcgtttc cagacgcggc atatttccgc cgaggaggct aataaactgc
      421 atctcggcgc ggcggaaacc tcgaacgtgc ggacggttta tcagtttatt attccgggca
      481 tttgccaatc gtcgcagctg gttttgggac tgacgtcgct ccagaccgga agcgtctgga
      541 acacttgccc gccgcacatt cacgaacggc gctcggaagt ttatttatat ttcgatttag
      601 acgaaaactc gcgcattatg cattttatgg gacagcccga agatatgagg catatcgtgc
      661 tggcgaacga agaagcggta gtatcgccgc cgtggtcaat ccatatgggc gcgggaacga
      721 gcaattacag cttcgtctgg gcgatgggcg gcgaaaatct cgattacacg gatatgaacg
      781 tgctggacat ctgccagctg aaataatcgg cgcaaacctc atcgagcggt ttttgaattc
      841 gccttccggt tcattgcgcc gacggcagca aaaaatccgg caaactaaaa aaaagggaat
      901 gcccgtctac aaaaacgggc attccctttt aaagcttcca aaagaaccgg catcaaccta
      961 aaagcggcta acggacgctg acgataacgt tcggtgccgt cgcgccgaac cactgcacgc
     1021 cttcctgcac cattttccac tgaaaattat atgtgccggg cgcggcgggc gccgtgacga
     1081 taaacacaaa agtttcggtt tcgttcggcg caaccggatt ctctaattga atgcggtttg
     1141 ttccccatgt ccagttgtcg cgcggatttt gcgagccgag cttgtaaatt ccgggcgtcc
     1201 aggtcgtgcc gcccgtgttt cgcatcgtaa cggaaaccgt ataggattgc ccggcggaca
     1261 tctgcgacgg aaccatctgc gaaacgaacg ccgagttgtt tccggcagcg tcgttgtcgt
     1321 caatcgtcaa aacggcggtg aacggtttta ccagaataga gccgccgacc ggattcgata
     1381 aagtcagatt cacggtctcg tttggctcgt ccgccgcgtc gttgataatc ggaatcgaaa
     1441 aacttttcgt cgtttcgccg tcggcaaaat acaagacgcc gcttctcgcc gtataatcct
     1501 gaccggcgag ggctgtgccg ttcgacgttg cgtaattgac gcttgccgcg cccgtcgaaa
     1561 ccgtgcgcgt tacgttaacg acggctgatg cgccggattc atcgaccgca taagtcgcgg
     1621 cggaaaagcg aaaagcgccc gctctggtga taaatacgtc ggcgcgaaaa cgcccgctgt
     1681 tgtttgcggc gtagctgtca ttgatgccga gccagagaga ttgggcggcg gtcgcggtaa
     1741 aactcgcgcc gtttgttccg ataaaaaagc gcgagccgcc gcgtttcata aataagcccg
     1801 cgtgaccgtg agccggagcg gtcagagggt ctgcgccgac ggttcggtcg ttgccgtaac
     1861 acggcaaatt acccggagtt tcataaacgc cgcgatttaa cccgccggca ttgacggtgt
     1921 aaattaaacc ggaattgacg cagctttgca gcggcgtctc gaacgtcacg gtgccggtga
     1981 cgtttgtaat aagcacgtcg tcgccgggct gcaggttggc gacgaattcc tcgttgcccg
     2041 cgattgaatc ggcttcgaca acgacgtttt gcttcgacga aggatgcgta atcgtcacgc
     2101 gggcattgaa ttgtccgctg ttgttattgc ctctcacgtc gttgacgccg aaccgcagcg
     2161 tctcagcgcc gagcgccgta aagctcgcgc cgttgcgccc gataaacgtc cgcgtggtgt
     2221 ttcgtttcat atacaatccg gcgtgcccgt gactcggtcc ggtcatcggg tctgccggat
     2281 tatcgagctg gtaatcgcaa agcaattgat tctgcggatt ttcaaacagc tccctcgcca
     2341 aaccattggc gttgactacg tgcgggcagc ctacggcaac cgcgtcagtc aaaaaatcga
     2401 cggtgcccga aatgttgtca atcaccacct ggtcgcccgc ggcgagcggc gcgccgaaga
     2461 cttcggcgga cgccgtgttc gtgccgcgca cgacgacgtt cagggtttgc acctgcgggc
     2521 gcttaatcgt cactttgacg ttgaacgcgc ccccgtttgg cgatgacggg caagccgcgt
     2581 cgttgacgcc gagccgcaaa tcgcgcgccg tcgtgccggt gtaggtaaaa gcgtcgttat
     2641 tatttccgag ccattttatc gagccgatgc ccggttgaga gggtcggaaa agtccggcgt
     2701 gattttgagc gggcagcggg tcgttttgat taatctgaca atagtttaaa tcctcgtccc
     2761 agcacgaacc ctgataaatt tccgaacgcg gcttgccgcc cggcgaagcg tcgtaagcgc
     2821 tgcccgtgca gatgcctgac gtgttgaaat cgacggtgcc gccggtcggg tcaatcgtta
     2881 ccatatcgcc gggcagaagc gtcacgcgcg ggacggtgta tccgccctga gcgccgacgt
     2941 ttgcgcccga aaccgtgacg ttcatcacaa tcgactgcgt cgaaaggatt tcgatttcct
     3001 ccggcggcgg cgctacctgc gccgagacgg tcgcggattt gtcaaaaata ccgccgagcc
     3061 agatgcccgg cacaaatgca aaacagatta acgctaaaat taaagatttg tgtttcataa
     3121 aaattctcct tcttaagctg ccggacgcgg taaagacctc ctgcgcccgg cgcgcgattt
     3181 tataattacc cgaaaagaaa cttcgttatt tttccttgtt taaatttggg acttgcaatt
     3241 attaacgcga cgggtttgaa aaaaaacgct caaaaaaaat ttgatatttg acgtgaattt
     3301 taaaaaaaac tatttttaaa ttcggtttat gaaactttcg cgcccgcttc ggtgtctatc
     3361 gattacagct aaaagtctga aaatccggga gtaaatatga gaaaattttc aattattttg
     3421 ataataattt gcgcggtcaa ttttgttttt gcccagacgg gcggcgaagt tctggaattt
     3481 aaatcaaaaa tcggttttac aggcggcgaa gataaaatcg tcgagtataa gtttctggaa
     3541 aacggcagaa agctcctgct tatcggagag aaaaatttgc agatttggga cgtcgaaaac
     3601 gccaggctgc tcaatgccgc aacgcaccag attccgcagt tcgcgccgcg cggttttttc
     3661 agcacgtatc tgcttatggg agtgccgaga cttttggatt ggcgtccgtt tttagttgac
     3721 gaaggcggaa gatggataat taccgtcgaa aaaatcggcg ccaatccgct gcgttccgcc
     3781 gtcgtgcgcg atttgcagaa tttaaaacaa atcgcggcgc tggatttgcc caacgtttcg
     3841 accgatt
//
LOCUS       DASWNE010000632         3426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_232642, whole genome shotgun sequence.
ACCESSION   DASWNE010000632 DASWNE010000000
VERSION     DASWNE010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232642"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..2226)
                     /gene="ligA"
                     /locus_tag="VGB68_12370"
     CDS             complement(211..2226)
                     /gene="ligA"
                     /locus_tag="VGB68_12370"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEY0050079.1"
                     /translation="MNLFEEAEQNIKTEIEALRAEINRHNELYYQKAAPEIADAEFDA
                     LLAKLKALEEENPEFITPDSPTRRVGGRAEGFKPFVHRVPLMSLDNSYDLEDLRAFDD
                     RVRRLADGRDFDYVAELKIDGLSVALHYENGVLAAGATRGDGTIGDDVYGNVKTIRTI
                     PLRLKGDFSEKVEIRGEVFLARSVFGKINAELEMQGEKIFANPRNCAAGTLRQLDASI
                     VAARRLDMFPYDVLSGNQKMFATHWAIFQWLEAKGFNVNPNRALCKNFDELGEFIDSM
                     QAKRDSLDYDIDGVVVKVNQTHLQQEFGATTKAPRWAIAYKYPAMQATTQLEKIEIQV
                     GRTGALTPVAYITPVQLAGTVVERASLHNEDEIKRLNLKINDWVLIEKSGEIIPQILQ
                     VIESKRTGVEKDYEFPKNCPICGADAVRPEGEVVTRCSNTDCPAKVKARILYYASRKA
                     MDIEGLGDILVKKLVDSGIVKNVADLYDLKLDEIAKLERMAEKSATNLINQIETSKTR
                     GLQRLLYGIDIRHVGERYAKILANHYRNIDKLAEASIEELDAIHEIGLTVAVSVYDFF
                     RNPRHQEVIRRLKAAGVTTEIDADASASFDENFAGKTFVLTGKLEQFTRDEAAKIIEE
                     RGGRIASLVSKKTDYVVAGEDAGSKLTKAESLGVKVLSEDGFGKMLG"
     gene            complement(2258..2827)
                     /locus_tag="VGB68_12375"
     CDS             complement(2258..2827)
                     /locus_tag="VGB68_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050080.1"
                     /translation="MANIKHLTTAELECGLEKIRQSPKDNGVLKLIVRRPQENKREVL
                     EKAGLDLFEGLIGDNWKTRGSSSTIDGLSHPDKQLNIMNARAIALIAQDENRWRLAGD
                     QLFIDLDLSDENLPPGTRLQIGTAIIEVTPPPHNGCKKFVARFGLDAMKFVNSPIGKQ
                     LRLRGINAKVVCAGLIRTGEPVKKIGWIL"
     gene            complement(2835..>3426)
                     /locus_tag="VGB68_12380"
     CDS             complement(2835..>3426)
                     /locus_tag="VGB68_12380"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY0050081.1"
                     /translation="FLGEMQKKIRVSDLENLRFAGARDYEVKAHWSLYCENYLEGFHL
                     PFVHQSLNEAIDFGSYTTETFRFSSLQTGFAKTDENVFDLPESNGKIAAFYFFIFPNL
                     MFNFYPWGVSVNIVKPVKPDLTIVSYLTFVSDESKLEKGAGADLNRVEMEDQSVVESV
                     QKGIRSRFYANGRYSPSREQGTHHFHRLIAEFMKAG"
BASE COUNT          780 a          926 c          791 g          929 t
ORIGIN      
        1 atcgcctgca aaaccgcctc ggacacgaaa ttttccgatg caattaattc tagaccgttc
       61 gcctgccgat tcgtttcatc attgatcgct ccggcaacga ttgaatcgac ttccgctaaa
      121 tttgcattaa aaagtttgtt catatttaat ctgaaaacta attattaccg attttctcaa
      181 aaactgattt acattttact atgaaaacct ttaaccgagc attttcccaa atccgtcttc
      241 gcttaaaacc ttgacgccca aactttcggc tttcgtcaat ttcgagccgg cgtcttcgcc
      301 cgcgacgacg taatcggttt ttttgctgac caaggacgcg atgcgaccgc cgcgctcttc
      361 aattattttc gccgcttcgt cgcgcgtgaa ttgttcgagc ttgccggtca aaacaaaagt
      421 ttttccggca aagttttcgt caaagctcgc gctcgcgtcg gcgtcgattt cggttgttac
      481 gcccgccgct tttaatcttc gaatcacttc ctgatggcgc ggattccgga aaaaatcata
      541 aacgctgacg gcgaccgtca agccgatttc gtgaatcgcg tcgagctctt cgatggaagc
      601 ttcggcgagt ttatcgatgt ttcgataatg attcgccaga attttcgcgt agcgctcgcc
      661 gacgtgccgg atgtcgatgc cgtataaaag ccgctgcaaa ccgcgcgttt tcgacgtttc
      721 gatttgattg attaaattcg tcgcgctctt ttccgccatt ctttcgagct tggcgatttc
      781 gtcgagcttc aaatcgtata aatccgccac gtttttgaca atgcccgaat cgaccagctt
      841 tttaaccaga atgtcgccca gcccttcgat gtccattgcc ttgcgcgagg cgtagtacaa
      901 aatcctcgct ttgactttgg cgggacaatc ggtattcgaa cagcgcgtca cgacttcgcc
      961 ttcgggacga accgcatccg cgccgcagat aggacaattt ttcggaaact cgtaatcctt
     1021 ttccacgccg gttcgtttcg attcgataac ctgcaaaatt tgtggaatga tttcaccgga
     1081 cttttcaatc aaaacccagt cattaatttt aaggttcaat cttttgattt catcctcgtt
     1141 gtgaagtgag gcgcgctcaa ctacagttcc ggcaagctgc accggcgtta tataagcgac
     1201 aggcgttaaa gcgccggttc tgcccacctg gatttcaatc ttttcgagct gcgtcgtcgc
     1261 ctgcatcgcc ggatatttat acgcgattgc ccagcgcggc gctttggtcg tcgcgccgaa
     1321 ttcctgctgt aaatgcgtct gattgacctt gacgacgacg ccgtcgatgt cgtaatcgag
     1381 cgaatcgcgc tttgcctgca tcgaatcgat aaattcgccc agctcgtcga aatttttgca
     1441 gagcgcgcga ttcggattga cgttaaagcc tttcgcttcg agccactgga aaatcgccca
     1501 gtgagtggca aacatttttt gatttccgct cagaacgtca tacggaaaca tatccagacg
     1561 ccgcgcggcg acaatcgatg cgtccaactg ccggagcgtt ccggcggcgc agttgcgcgg
     1621 gttggcgaaa attttttcgc cctgcatttc cagctcggcg ttgattttcc cgaacaccga
     1681 gcgcgccaga aaaacctcgc cgcgaatttc gactttttcg gaaaaatcgc ctttcagacg
     1741 cagcggaatc gtgcgaatcg ttttgacgtt gccgtaaacg tcgtcgccga tcgtgccgtc
     1801 accgcgagtt gcgcccgccg ccagcacgcc gttttcgtaa tgcagcgcga ccgaaagacc
     1861 gtcgattttc agctcggcga cgtaatcgaa atcgcgcccg tcggcgagcc gccggactcg
     1921 gtcgtcaaac gcccgcaaat cctccaaatc ataagaatta tcgagcgaca tcaaaggcac
     1981 tcgatggacg aaaggcttaa agccttcggc gcgcccgccg actcgacgcg tcgggctgtc
     2041 gggcgtgata aattccgggt tttcttcttc cagagctttg agcttcgcca aaagcgcgtc
     2101 aaattccgcg tcggcgattt ccggcgcggc tttctggtaa taaagctcgt tatgccggtt
     2161 gatttcggcg cgcagagctt cgatttcggt tttgatgttt tgttccgctt cttcaaaaag
     2221 gttcatagaa aaattctaac ccgaaaggct ctaagattta taaaatccat ccgattttct
     2281 taaccggctc gcccgttcga atcaagcccg cgcaaacaac tttagcattt atgccgcgca
     2341 ggcggagttg tttcccgatt ggcgaattga cgaatttcat agcgtcgagc ccgaagcgcg
     2401 cgacgaattt tttacagccg ttgtgcggcg gcggagtaac ttcgattatc gccgtaccga
     2461 tttgcaaacg cgttcccggc ggcaaatttt catcgctcaa atccaaatct ataaaaagct
     2521 ggtcgcccgc cagccgccat cgattttcat cctgcgcgat gagagcaatc gcccgcgcat
     2581 tcataatatt taactgtttg tccggatgcg ataagccgtc gattgtgctg gaactgccgc
     2641 gggttttcca gttgtcgccg attagtcctt caaataaatc aagccctgct ttttccagaa
     2701 cttctctttt attttcctgc ggtcgccgga cgatcagctt caaaacgccg ttatctttgg
     2761 gcgattgacg aatcttttct aatccgcatt caagctctgc cgttgtcaag tgtttgatat
     2821 ttgccatttt tgttttatcc ggctttcata aattccgcaa tcagacgatg aaaatgatgc
     2881 gttccctgct cgcgcgacgg cgaatatctg ccgttcgcat aaaaccgcga gcgaattccc
     2941 ttctgcaccg attcgacaac cgactggtct tccatctcga cgcggtttaa atccgcgccc
     3001 gcgccttttt ccagtttcga ttcgtcggag acgaaagtca gatatgaaac aatcgtcaaa
     3061 tcgggtttta ccggcttgac tatatttacc gacacgcccc acggataaaa gttaaacatc
     3121 agattcggaa agataaaaaa gtaaaacgcg gcgatttttc cgtttgattc gggcaaatcg
     3181 aaaacgtttt cgtccgtttt ggcgaaaccc gtctgcaggc tcgaaaagcg aaaagtttcc
     3241 gtcgtgtagc tgccgaaatc aatcgcttcg tttaaggatt gatgaacgaa cggaaggtga
     3301 aagccttcca ggtaattttc gcaatacaaa ctccagtgag ctttgacttc ataatcccgc
     3361 gcgccggcaa agcgcaggtt ttcaaggtct gacacccgaa tttttttctg catttcgccg
     3421 agaaaa
//
LOCUS       DASWNE010000633         3021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_403753, whole genome shotgun sequence.
ACCESSION   DASWNE010000633 DASWNE010000000
VERSION     DASWNE010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403753"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            322..1419
                     /locus_tag="VGB68_12385"
     CDS             322..1419
                     /locus_tag="VGB68_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050082.1"
                     /translation="MQNAPEIKRENINRFLCENCGANMVFNPQNGLLTCPYCAHAQEI
                     EAGGAVEERDYDRFLQAGAQNLQPMAQDAMQVGCDSCGAIVNFTPPETARNCDFCGAK
                     IVAQPKSADPLIAPEGVLPFRITDKQATEGLRNWINSRWFAPSKLKDLARPDKLSSIY
                     IPYWTYDAFSQSSYAGERGEHYYETEHYEENGERKSRQVRHTSWSSASGYVSRQFDDV
                     CVPATKSLSKSHLVNLEPWDLQELKAYEPAFLSGHKAQTYQVALPEGFEVFKQIADGV
                     IHSDVRQDIGGDEQRVHNISTDFSNITFKHLLLPVYAGAYQFNNKTFQIVVNGRTGEV
                     QGDRPYSWIKITLLVLFIILIIAVLVMIFGR"
     gene            complement(1474..2358)
                     /locus_tag="VGB68_12390"
     CDS             complement(1474..2358)
                     /locus_tag="VGB68_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050083.1"
                     /translation="MKKTFFSLTFIFIMSVAVYAQTTTGDTNTSITTRTATPTPTPTR
                     PRATPTPVTPVRQLTAEEQQRQRESSERRRQEAATGDPDVLLDVPNLSVEEINLEVEN
                     LRAKVSLDARLANLLQLSAGADVGIDKVKLTIKGVRAEVLLKVRLDNVASIIDRTLTT
                     IDRNPQILERLLTSVDNTVNTVGNVANTALQPGGVLSQTVNTLGQTVQRTLDTTGNIV
                     EKTLDTTGRVVNQRTLGSVLDTTATGLRVVNETTNNAGQTVRRLQDASGGVIEVVVDS
                     AGKIVSSRIVSGAGVQPR"
     gene            complement(2428..2796)
                     /locus_tag="VGB68_12395"
     CDS             complement(2428..2796)
                     /locus_tag="VGB68_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050084.1"
                     /translation="MNSTALLRVLFFACVLFTASLIFSADARGQDKNTPPIDTADEDF
                     ELNIDDSRTNESNYRRSTSVEINEANVSVGVGAAVSAQKIDLILRGVTGRVRFRASLE
                     AIRRRIAQANQTRPQNSGNR"
     gene            complement(2807..>3021)
                     /locus_tag="VGB68_12400"
     CDS             complement(2807..>3021)
                     /locus_tag="VGB68_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050085.1"
                     /translation="NIGIRLRIYSRFAEIQRIVLEAIDEEPNSTAVNQTSPAETPQDK
                     AAALPSASPIAQKPKPKTNRTGQARK"
BASE COUNT          664 a          805 c          826 g          726 t
ORIGIN      
        1 gcgcgcccga cacaaagaca accgtttcca gcagaaattt atcgcgaaat ttgttgagca
       61 tcgattcgat tgagccacaa tttcaacgaa aagacaaaga ttaaccacag agcgcagagg
      121 gaaattaacc acagatttgc acggataaac acgggtgatt aagtcaggat ttgcggaata
      181 aaatctttta cctatatctt cttatccgcg ctaatctgtg taaatctgtg gtttcatatt
      241 cttaaaaatc tgtgtgctct gtgttgaaaa tctttcctga gttgagaatc tgcaaatttt
      301 agtttaattt tctgaaagtc tatgcaaaac gcacctgaaa tcaaacgcga aaacatcaac
      361 cgttttttat gcgaaaactg cggcgcgaat atggttttca atccgcaaaa cgggcttttg
      421 acctgtcctt actgcgcgca cgcgcaggaa atcgaggcgg gcggcgcggt cgaagaaaga
      481 gattacgacc gctttttgca ggcgggcgcg cagaatcttc agccgatggc gcaggacgcg
      541 atgcaggtcg gctgcgacag ctgcggcgca atcgtcaatt ttacgccgcc cgaaaccgct
      601 cgaaactgcg atttctgcgg cgcgaaaatc gtcgcccagc cgaaatccgc cgacccgctg
      661 attgcgcccg aaggcgttct gccgtttcgc atcacggaca aacaggcaac cgaaggcttg
      721 cgaaactgga taaactcgcg ctggtttgcg ccgtcgaagc tgaaggattt ggcgcgtccc
      781 gacaaactga gcagcatcta cattccgtat tggacgtacg acgctttttc gcaaagctct
      841 tacgcgggcg agcgcggcga gcattattac gagaccgagc attacgagga aaacggcgag
      901 cgaaagtcac gacaggtgcg ccatacgagc tggtcttcgg cgagcggata tgtttcgcgg
      961 cagtttgacg acgtttgcgt tccggcgacg aaatccctgt cgaaaagcca tctggttaac
     1021 ctcgaaccgt gggatttgca ggaattgaaa gcttacgaac ccgcgttttt gtcgggacac
     1081 aaagcgcaga cttatcaggt cgcgctgccg gaaggctttg aagtcttcaa acaaatcgcc
     1141 gacggcgtga ttcacagcga cgtccggcag gacatcggcg gcgacgaaca gcgcgttcac
     1201 aacatctcga ccgatttttc caacattact tttaaacatc tcctgctgcc ggtttacgcg
     1261 ggcgcgtatc aattcaacaa caaaacgttt caaatcgtcg tcaacggcag aaccggcgaa
     1321 gtgcagggcg accgcccgta tagctggata aagattacgc tcctggtgct gtttatcatt
     1381 ctgataattg ccgttctggt gatgattttc ggcagataaa taaaaaggcg ggaattaaag
     1441 cccgccttaa ttattcccga taaaaaaatt ccattatctg ggttgaacgc ccgcgccgct
     1501 gacgattctg gagctgacga ttttcccggc ggaatccacg acgacttcga tgacgccgcc
     1561 cgaagcgtcc tgcaagcggc ggacggtttg cccggcgttg ttggtcgttt cgttgactac
     1621 tcgcagcccg gtcgccgtcg tgtccagaac cgagccgagc gttctttgat tgacgactct
     1681 gccggtcgta tccagagttt tttcgacgat atttccggtc gtgtccagtg ttctctgaac
     1741 ggtttgaccg agcgtgttga cggtctggct taaaactccg cccggctgca atgccgtatt
     1801 cgccacgttt ccgaccgtat tgaccgtgtt gtcgaccgac gttagaaggc gttcgagaat
     1861 ctgcgggttg cggtcgatcg tcgtcaaagt gcggtcgatg atgctcgcta cgttatccag
     1921 acgcactttc agtaaaactt ccgcccggac gcctttaata gtcagtttca ccttgtcgat
     1981 tccgacgtcc gcgcccgccg acagctgcaa aagatttgcc agccgcgcgt cgagcgagac
     2041 tttggcgcgc aggttttcca cttccagatt tatttcctcg accgaaagat tcggcacgtc
     2101 gagcagaacg tccgggtcgc ccgtcgccgc ttcctgccgg cggcgctcgc tggattcgcg
     2161 ctgccgctgt tgttcttcgg cggtcagctg acgaacgggc gtcacgggcg tcggcgtggc
     2221 gcgcggtcgc gtcggcgtcg gcgtcggcgt cgcggttcgc gtggtgatgc tcgtattagt
     2281 gtcgccggtc gtcgtttgcg cgtaaacggc gacgctcata ataaagataa aagttaagct
     2341 gaaaaaagtt tttttcatcg gtttcctcct cgttaaaagc cgcgcctgaa atcgattccg
     2401 ggcgcggcaa acagcaatta aaatttttta gcgattgccc gaattttgcg ggcgcgtctg
     2461 attggcttgt gcgatgcggc ggcgaatcgc ttcgagcgaa gcccgaaagc gaactctgcc
     2521 cgtcacgccg cgcagaataa ggtcgatttt ctgcgcgctg accgccgcgc cgacgccgac
     2581 cgaaacgttt gcctcgttaa tttcgacgct ggtcgagcgc ctgtaattcg attcgttcgt
     2641 gcggctgtcg tcgatgttca gctcaaaatc ttcgtccgcc gtgtcaatcg gcggcgtgtt
     2701 tttatcctgc ccgcgcgcgt cggcggaaaa aatcagagac gccgtgaaca gaacacaggc
     2761 gaaaaacaaa acgcgtaaaa gcgccgttga attcatcagg ttctccttat tttctcgctt
     2821 gtccggttcg gtttgttttg ggtttcggct tttgggcgat cgggctcgcg gacggcagcg
     2881 cggcggcttt gtcctgaggc gtttccgcag gcgaagtttg attgaccgcg gttgaattcg
     2941 gttcttcgtc aatcgcttcc agcacgatgc gctgaatctc ggcgaaacgg ctgtaaatac
     3001 gcagccgaat gccgatattt c
//
LOCUS       DASWNE010000634         6148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_232643, whole genome shotgun sequence.
ACCESSION   DASWNE010000634 DASWNE010000000
VERSION     DASWNE010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6148
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232643"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..327)
                     /locus_tag="VGB68_12405"
     CDS             complement(166..327)
                     /locus_tag="VGB68_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050086.1"
                     /translation="MPPEIFPVYSRAGFQVNAERERQQPNRQRADSENDERKNLRKTT
                     VSAETFLHV"
     gene            430..2064
                     /locus_tag="VGB68_12410"
     CDS             430..2064
                     /locus_tag="VGB68_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050087.1"
                     /translation="MQENREKFSFPTRSYSYRSATRKANAFEAEPQLEAGRIYRFVMG
                     VDNFPGLMPETEVETLLRDSFAELLLKRGTFPLTVRDLLSALDALNDNSDGLPQQKVF
                     LVADGGQIDWTPETANVNRLFRFAVARSRNDDARLLVSASTALDSETQFLQVLSWDAA
                     NQVYSYYERRGGAWIWAGNSHHALLPGSRGAGPFDSHVNGSLVMKELRAPWCNWNSMN
                     AAIREDALAPDDALRAEPLFLNRASASELERNVVRPGIERWNRARLQHSLSEDGKTLR
                     DMPFFLRQILQTTTVNLASSSRQSNRVGDDEPMDLPITFFLNTEALLDYVELDPDISP
                     ISIAGKFYNDSLRRYDFTLRDGEFSQKGDTFFGFLIPEPAFEDLNLLALLLERKIISR
                     RFAACLLMIDFPNPVFSAKRAQMMQFVPPTANITGGALKSDFETQFTAILESVIMKMP
                     ASHAAHEFLANWRLPEKDWKRTFESRIEDYFNALQEKSRTDEGFDAWTRLAESRRREF
                     RGHPLAEFRLTTPSTNIPEDAPLLEMRADAAVRAKR"
     gene            2100..3218
                     /locus_tag="VGB68_12415"
     CDS             2100..3218
                     /locus_tag="VGB68_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050088.1"
                     /translation="MLLEKFDPPGFLRDFNEEQGGAWSQFISQQFDEAAAGFPDSFEF
                     DAPRAQFYNPTKVETGEDAQTAVISWTAFPRNVTVTSVSDLQRWRRAEASRDRQDEYC
                     EWSVERDAETDKITRVSFTCEGPEYWDFLANTTPEVALALYQEFISPEVRMEDLFGAD
                     GRYNRRNRWNDSTTGGAMHLIQQNNTLGAEIELAGGASVVREIDGRILTGEDELIRCG
                     RYGGVERHSDPHIGAIVNSVTRQKADVTLGNPVGLYLNDLNVAGWETPDGSDPKSFWK
                     YVRGTGEFPVRAVFEVPAERGYTVGDIKIGGREIAFGAQIVDFITIKLVGVGTRFGQS
                     TVKPMTGCRIRRPQHSFNAPELKVQDFIGRSSGKTTRS"
     gene            complement(3457..3918)
                     /locus_tag="VGB68_12420"
     CDS             complement(3457..3918)
                     /locus_tag="VGB68_12420"
                     /inference="COORDINATES: protein motif:HMM:NF040141.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5675 family protein"
                     /protein_id="HEY0050089.1"
                     /translation="MADEIITPTIYLIRTKVFVTSKSDDGEAAIGERGILVAGTVSYH
                     ALERRGGYVTLPSGEFEIEMEHSPTKKVNGKPRRQFRVLNHGVYNNQGKLAAILMHQG
                     NYPHHVTGCIAPGKSQSAKGIGQSTKALEEIFTFCGGFGVGKRALLVIDGD"
     gene            complement(4039..4212)
                     /locus_tag="VGB68_12425"
     CDS             complement(4039..4212)
                     /locus_tag="VGB68_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050090.1"
                     /translation="MTEKTKPQNDKAPKEAVEKPETGDPASWSRDQQEKSYYYDDDCG
                     YEVYDPDEDDEED"
     gene            4256..5095
                     /locus_tag="VGB68_12430"
     CDS             4256..5095
                     /locus_tag="VGB68_12430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HEY0050091.1"
                     /translation="MKTRNYLLSFGLILASVVSTYFAATSAAQQGAQTQAAADNEYQI
                     GAILYMQKAAEYRALAFQAFNIARIQLDADAKDRKKLPKAERKMPRAVVVDVDETVLD
                     NSPAQAVQVKKRLPFIQENWTAWVNMRKAKAIPGAIDFLNYASRNSARIFYVTNRIEA
                     EKQPTIDNLKALGFPDVSPETVLVKTAESSKEARRQSILQKYRIAVLIGDNLDDLSNV
                     FERKSIDARFAEVDKTREMFGRKFIVLPNAMYGTWEGAIYEYGRLTEAQKTEKRVNAL
                     ESY"
     gene            5145..>6148
                     /locus_tag="VGB68_12435"
     CDS             5145..>6148
                     /locus_tag="VGB68_12435"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEY0050092.1"
                     /translation="MLPKKIIAFCLFTFAFCLVLSANGQNLTVRDIMREPSMAGMRPD
                     SEKLSPDGRRIVFAWNAEGREPRNLYIVDSAGGEPRILVNAEQNFEARTPPPESKLNY
                     GLAVRDDFVRAREKNLGGVEFSPDSKRILFLQNTDIYVLDLEAKDAKPRRITRTQGTE
                     ASARWLTGDAILYQSGGNFFVLNLKETSLIQVTKEANPQAFVTIFNAGATEDGRLLAY
                     VLSDGSRQRALFVPNYLDEFTQAPTTRRGFTEQKVFVTLTDGSREKPFEINLPKAEGV
                     SFVRSLRWAADASSLLVDRVDKDTKRRQLFFVHDAGSKAEQIILVTEEADAKWIAPLS
                     R"
BASE COUNT         1377 a         1645 c         1700 g         1426 t
ORIGIN      
        1 acaaaagata ccgaacatca atttagaatt tacaatttac aattttccct ttctttattt
       61 ttgtgcaaag cccttcggct tgtttttcaa ctgcaatctg aattatgatg tgtttccgat
      121 ttcaggctct gcgcggcgga aaatatgtat tgtgcggctc gcggctcaga cgtgcaggaa
      181 ggtttcggct gaaacggtag tttttctcag gtttttccgc tcgtcgtttt cggaatcagc
      241 gcgctgccga ttcggctgct gtcgctcgcg ttcggcgttt acctgaaaac cggcgcggct
      301 gtagacggga aaaatttccg gcggcaaaac caaacttttt ttgttaaaaa tcgctttgtc
      361 tttattgcct ttccaaatta ataaagcttt tattcaattc agcgccgtcc gggctgaaaa
      421 ttactgatta tgcaggaaaa cagagaaaaa ttcagctttc ccacgcgcag ttattcttat
      481 cgctctgcga cgcgaaaggc gaatgcgttt gaagccgagc cgcagctcga agccggacga
      541 atctatcgct ttgtaatggg cgtcgataat tttcccggat tgatgccgga aaccgaggtg
      601 gagacgcttt tgcgcgattc tttcgccgaa ttgctgctca agcgcgggac gtttcctttg
      661 accgtgcgcg atttgctgtc cgcgctcgac gctttaaacg acaactctga cgggctcccg
      721 cagcaaaaag tttttctcgt cgccgacggc gggcaaatcg actggacgcc ggagacggca
      781 aacgtcaacc ggctgtttcg tttcgccgtc gcccgctcgc gcaatgacga cgcgcggctg
      841 ctggtcagcg ccagcacggc gctggattcg gagacgcagt ttttgcaggt tttatcgtgg
      901 gacgcggcga atcaggttta tagttattac gagcggcgcg gcggcgcgtg gatttgggcg
      961 ggcaattcgc atcacgcgct tttgcccggc tcgcgcggcg cgggtccgtt tgacagccac
     1021 gtcaacggct cgctggtgat gaaggagctg cgcgcgccct ggtgcaactg gaacagtatg
     1081 aacgccgcga ttcgagagga cgcgcttgcg ccggacgacg cgctgcgcgc cgagccgctg
     1141 tttttgaatc gagccagcgc cagcgaattg gagcgcaacg tcgtgcgtcc cggcatcgag
     1201 cgctggaatc gagcgcggct tcagcattcg ctctccgagg acgggaaaac tctgcgcgat
     1261 atgccgtttt tcctgcgcca gattttgcag acgacgaccg tcaatctcgc cagctccagc
     1321 cgccagagca accgcgtcgg cgacgacgag ccgatggatt tgccaatcac gttttttctg
     1381 aataccgaag ccttgctcga ttacgtcgag ctcgacccgg acatttcgcc gatttcaatc
     1441 gcgggcaaat tctacaacga cagcctgcgg cgctacgatt tcaccttgcg cgacggcgaa
     1501 ttttcgcaaa aaggcgacac ttttttcggt tttctcattc ccgagccggc ttttgaagat
     1561 ttaaatctgc tcgcgcttct tctggaaagg aaaattatca gccgccgttt cgccgcgtgt
     1621 cttttgatga tcgattttcc gaacccggtt ttttcggcaa aacgcgcgca gatgatgcag
     1681 ttcgttccgc cgacggcgaa tattacgggc ggcgcgctga aatcggattt tgaaacgcag
     1741 tttacggcga ttctcgaaag cgtgattatg aaaatgcccg ccagccacgc ggcgcacgag
     1801 tttctggcaa actggcgctt gccggaaaag gactggaagc ggacttttga aagccgaatc
     1861 gaagattatt tcaacgccct gcaggaaaaa tcccgcaccg acgaaggctt tgacgcttgg
     1921 acgcggctgg cggaatcgcg gcggcgggag tttcgcggtc atccgctcgc cgaatttcgt
     1981 ctgacgacgc cctcgaccaa tatcccggaa gacgcgccgc tgctggagat gcgggcggac
     2041 gccgcggttc gggcaaagcg gtaaatcgag gcttagtttc aaagtaaaaa ggagaaaaaa
     2101 tgcttttaga aaaatttgac ccgcccggat ttctgcggga ttttaacgag gaacagggag
     2161 gcgcctggag ccagtttatc agccagcagt ttgacgaagc cgccgccggc tttcccgata
     2221 gctttgaatt cgatgcgccc cgcgcgcagt tttacaaccc gaccaaagtg gaaaccggcg
     2281 aagacgcgca gaccgcggtt atcagctgga cggcttttcc gcgcaacgtg acggtcacgt
     2341 cggtcagcga tttgcagcgc tggcggcgcg ccgaagcgag ccgcgaccgg caggacgaat
     2401 actgcgaatg gagcgtcgag cgcgatgccg aaaccgacaa aatcacccgc gtcagcttca
     2461 cctgcgaagg tccggaatac tgggattttc tggcgaacac gacgccggaa gtcgcgctgg
     2521 ctttgtatca ggaatttatc agcccggaag tcaggatgga agatttgttc ggggcggacg
     2581 ggcgctacaa ccggcgcaac cgctggaacg attcgacgac gggcggcgcg atgcacctga
     2641 ttcagcaaaa taacacgctc ggagcggaaa tcgagctggc gggcggagcc agcgtcgtgc
     2701 gcgaaatcga cggcagaatt ctgacgggcg aggacgagct gattcgctgc ggtcgatacg
     2761 gcggcgtcga gcgtcacagc gacccgcaca tcggagcgat agtcaacagc gtgacgcggc
     2821 agaaagccga cgtgacgctc ggcaatccgg tcggattgta tcttaacgat ttaaatgttg
     2881 ccggctggga gacgccggac ggctcagacc cgaagtcttt ctggaaatac gtccgcggca
     2941 cgggcgaatt ccccgttcgc gccgttttcg aagtgcctgc cgagcgcggc tacacggtcg
     3001 gcgacatcaa aatcggcggg cgcgaaatcg ctttcggcgc gcaaatcgtc gattttatca
     3061 ccatcaaact ggtcggcgtc ggcacgcgct tcggacaaag cacggtcaag ccgatgaccg
     3121 gctgccgtat tcgccgcccg cagcacagct ttaacgctcc ggaattgaag gtgcaggatt
     3181 ttatcggtcg gtcgagcgga aaaacgacca ggtcttgaca cggctttcgc tgccggcggc
     3241 gaatatagaa caaagcgtcc gaaaagcatt gattttcgga cgctctgcag gcaaagtcga
     3301 aaatcgattt tagcggaagg gcagcgggaa aggtaaatta tttgtcttga aagaagcgcg
     3361 tttttattca aacgaacgac gtccggaata atccgctcgc gccgcttgaa aacattttcg
     3421 ggcttttcga tttattaaaa attccgtgtt tttgttctag tcgccgtcga tgaccagcag
     3481 cgcgcgtttt ccgacgccga aaccgccgca aaacgtaaag atttcttcca gcgctttcgt
     3541 gctctgcccg atgccttttg cggattgcga tttgcccggc gcgatgcagc ccgtcacgtg
     3601 atgcgggtaa tttccctggt gcatcaaaat cgccgccagc tttccctggt tgttatagac
     3661 gccgtgattc aaaacgcgaa actgccgccg cggcttgccg ttaacttttt tggtcgggct
     3721 gtgctccatc tcgatttcaa actcgccgga cggcagcgtc acgtaaccgc cgcgccgttc
     3781 cagcgcgtgg tagctgaccg ttcccgccac cagaattccg cgctcgccaa tcgccgcttc
     3841 gccgtcatcg gacttgctgg taacaaatac tttcgtgcga ataagatata tggtcggggt
     3901 aataatctcg tcagccattt tttctgttaa ggaagttaag gaagctaagg aaattaagga
     3961 aagcgggaat gagaatattt tctcctgctc ccttaatttc cctaatttcc tatttccctg
     4021 atttcccttt ctctcctttt aatcttcctc gtcgtcttcg tccgggtcgt aaacttcgta
     4081 gccgcagtcg tcgtcataat aataactttt ttcctgctgg tctcggctcc aagaggctgg
     4141 gtcgccggtt tccggttttt ctacggcttc tttcggcgct ttgtcgtttt gcggcttcgt
     4201 tttttcggtc atacatcgtt tataatctca acaaaaatct acgttcgaca aatttatgaa
     4261 aacaagaaat tatcttttaa gcttcggact aattttagcc tccgtcgttt caacctattt
     4321 tgcagcaact tccgccgcgc aacaaggcgc gcagacgcaa gccgccgccg acaatgaata
     4381 tcaaatcggc gcgattcttt atatgcagaa agcggcggaa tatcgcgcgc tcgcctttca
     4441 ggcgtttaat atcgcccgaa ttcagctcga cgccgacgcg aaagaccgga aaaaactgcc
     4501 gaaagccgag cgaaaaatgc cgcgcgcggt cgtcgtcgat gttgacgaaa cggttctgga
     4561 caattcgccc gcccaagccg tgcaggtcaa aaaacgtctg ccttttatac aggaaaactg
     4621 gacggcttgg gtgaatatgc gaaaggcgaa agcgattccc ggcgcgattg attttctcaa
     4681 ttacgcgagc cgcaacagcg cgcggatttt ttatgtcacc aatcgcatcg aagcggaaaa
     4741 acagccgacc atcgacaatc tgaaggcgct cggctttccc gatgtttccc cagagaccgt
     4801 tctcgtcaaa acggcggaat cgagcaagga agcgcgtcgg cagtcaattc tgcaaaaata
     4861 ccgcatcgcc gttttaatcg gcgataatct cgacgattta tcaaacgttt tcgagcgcaa
     4921 gtcgattgac gcgcgttttg ccgaagtcga taaaacgcgc gaaatgttcg ggcgaaaatt
     4981 tatcgttctg ccgaacgcga tgtacggcac gtgggaaggc gcgatttacg aatacgggcg
     5041 tctgaccgaa gcgcagaaaa cggaaaaacg cgtaaatgct ctggaaagct attaaacgaa
     5101 atctgttgtt tcagataatg cagtaatatt tcagctaaaa ttttatgttg ccgaaaaaga
     5161 taatcgcctt ttgccttttt acttttgcct tttgtctcgt tttgagcgcg aacggtcaaa
     5221 atctgactgt ccgcgacatt atgcgcgagc cttcgatggc gggaatgcgt cccgattcgg
     5281 aaaaactttc gcccgacggt cgccggattg ttttcgcctg gaacgccgaa ggacgagagc
     5341 cgcgcaatct ttatatcgtc gattccgcgg gcggcgagcc gcgcattctg gtcaacgccg
     5401 agcaaaattt tgaagcgcgc acgccgccgc ccgaaagcaa gctgaactac ggcttggcgg
     5461 tgcgcgacga tttcgtccgg gcgcgcgaga aaaatctcgg cggcgtcgaa ttttcgcccg
     5521 actcgaaacg cattttgttt ttgcagaaca cggatattta cgttctggat ttggaggcga
     5581 aagatgcgaa accgcgccgc atcacgcgca cgcagggcac ggaagcttcg gcgcgctggc
     5641 tgaccggcga cgccattctg tatcaatccg gcggaaactt tttcgtcttg aatttaaagg
     5701 aaacatcttt gattcaggtc acgaaagaag ccaatccgca ggctttcgtg acgattttca
     5761 acgccggcgc gaccgaagac ggaagactcc tggcgtatgt tttgagcgac ggctcgcgcc
     5821 agcgcgcgct tttcgtgccg aattatctgg acgaattcac gcaggcgccg acgacgcggc
     5881 gcggttttac cgagcagaaa gttttcgtca cgctgacgga cggaagccgc gaaaagcctt
     5941 ttgaaataaa tttgccgaag gcggaaggcg tttcgtttgt ccgcagcctg cgttgggcgg
     6001 cggacgcgtc gagcctgctc gtcgaccgcg tcgataaaga cacgaaacgc cgtcagcttt
     6061 tctttgttca cgacgcgggc agcaaagccg agcagattat tttagtgacc gaggaagcgg
     6121 acgccaaatg gattgcgcct ttgtcgcg
//
LOCUS       DASWNE010000635         5145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_269883, whole genome shotgun sequence.
ACCESSION   DASWNE010000635 DASWNE010000000
VERSION     DASWNE010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5145
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269883"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..2236)
                     /locus_tag="VGB68_12440"
     CDS             complement(152..2236)
                     /locus_tag="VGB68_12440"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEY0050093.1"
                     /translation="MQKFLTGIFLIFALLSSALSVVSAQENALQSARRNLMPVPLSVS
                     WKTGRLPVTKAFAVAVKGQTDERLKKYIFRVMRRLEGRTVLEFSRELSNDAAGANLLI
                     ETQSTGNAIPKLGDDESYNLEISENQARLTAPTTVGAMRGLETFLQLLEGDKNGFYFP
                     AVSISDRPRFPWRGLMIDSARHYQPMEVLRRNLDAMAAVKLNVLHWHLTEDQGFRVES
                     KKMPELHALGSDGLFYTQNEIREIIDYAAERGIRVMPEFDMPGHATAWLVSHPEIGSA
                     PGPYKIERRPGIFDPTLDPTNEKTYKLLKVFFKEMSALFPDAYMHIGGDENKGKQWTE
                     NPKIQAFMKEKGIRDNHALQTYFNRRLLELLQDNGKIMMGWDEIFQPDLPKDIVIHSW
                     RGQKALAEAARQGYQSVLSNGYYIDLVFPASQHYAVDPLPADTTLTAEEQKRVLGGEA
                     TMWSEWVSPETIDSRIWSRTAAIAERFWSPREVNDADDMYRRLAVIDFQLEELGLTHR
                     KNQLMMLRRLAKAQDVAALQTLVSVIEPVKEYRRYRMRPQTMLSPLTGLIDAAQPDAP
                     GARAFNKAVDEMLAGKNTSENAAKIRLMINVWRASETDLQKIMENSPALAEAGQLAAD
                     FRDLNQIALEALDSLDKKAAQNAEWRDAKLKRLDEIAKPKAALEIVVVSGVKKLINAA
                     AIGNVPSGTGAN"
     gene            2630..4228
                     /locus_tag="VGB68_12445"
     CDS             2630..4228
                     /locus_tag="VGB68_12445"
                     /inference="COORDINATES: protein motif:HMM:NF010069.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter ATP-binding protein"
                     /protein_id="HEY0050094.1"
                     /translation="MQRATDNERPADALLEMRSIGKEFPGVRALDGVTLDLYEGEFHA
                     LVGENGAGKSTLMKVLSGVYPAGSYDGEILIAGEVKQFKTIRDAENAGIAIIFQELSL
                     VKELTVGENIFLGREPSRFGVINWAELYQKAGTLLQELHLAIDPRTPVGNLGIGQQQL
                     VEIAKALSQKAKILVLDEPTAALTEAEVKTLFAILEKLRARGVGMIYISHKLGEVFRM
                     SDRITVLRDGKTVGTNETANLNIEKVIALMVGREVGDIFPKTNHQFGEIALEIKNLSA
                     FDLDNPNKKLVDDVSFNVRKGEVLGIAGLMGAGRSELLMSIFGGWQGKSRGEIYVEGR
                     QIFIGAPSDAIRHGIGFVTEDRKRFGLLLEQTILDNMTLAGLKQMSGKMLTHRSRETV
                     AASGAMQSLRVKAGSPLTVAGTLSGGNQQKVVLGKWLLTNPKILFLDEPTRGIDVGAK
                     QEIYTEINKLAREGLAIVLVSSELPEVLGLSDRVIVLHEGRLTGEFTRTEATAEKVMK
                     AATGNVQQTSPPRPEDEINRELSG"
     gene            4326..>5145
                     /locus_tag="VGB68_12450"
     CDS             4326..>5145
                     /locus_tag="VGB68_12450"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050095.1"
                     /translation="MNEETKISGLKFQTSSLRAYTMLGALILIWLLFYWQTGGTFLTP
                     RNLSNLMLQTSVTGILAVGMLMVILAGQIDLSVGSVLGLAGGVAAISLTNWDYGLAGS
                     ILLAIAVGVAIGLIHGTLTAYFNIPAFIVTLGGLLAWRGAIKGISRSETVPIADPTFK
                     LIGQGNITANSGWVLAGIGILLILFLAYRRAQAQKDYGLGEANYGGEMLKTVLPIGVI
                     IGFIYVMNSSDGVPIPVLIFLGVAIFGVFITNSTTFGRYLYAIGGNSDAARLSGI"
BASE COUNT         1140 a         1332 c         1401 g         1272 t
ORIGIN      
        1 cgagcgaaaa agctgcgccc gcgccgccga ttgccgtcaa aaattcgcgc ctgttgaaat
       61 tgtttttttt acgctgcata agtgatttga taggaatttg aacacgcctt tcgcgacgcc
      121 gcccgcgctt ctttcagaaa atttaacttt ttcaattcgc gccggtgccc gacggaacat
      181 tgccgattgc ggcggcattg attaatttct taactcccga tacgacgacg atttccagcg
      241 ccgctttcgg tttggcgatt tcgtcgagcc ttttcaattt cgcgtcgcgc cactcggcgt
      301 tttgcgccgc ttttttgtct aaagaatcga gcgcttcgag ggcgatttga ttcaaatcgc
      361 gaaaatccgc tgccagttgt ccggcttcgg cgagagcggg cgaattctcc ataattttct
      421 gcaaatccgt ttccgacgcg cgccagacgt ttatcatcaa ccgaattttc gccgcgtttt
      481 ccgaagtgtt ttttccggcg agcatttcat cgaccgcttt gttaaacgcg cgcgcgccgg
      541 gagcgtcggg ctgcgccgcg tcgattaatc cggtcagcgg cgaaagcatc gtctgcgggc
      601 gcattcgata acgcctgtat tctttgaccg gctcgatgac cgaaacgagc gtttgcagcg
      661 cggcgacgtc ctgcgctttc gccagccgcc gcagcatcat caattgattt ttgcgatgcg
      721 tcagacccag ctcttcgagc tgaaaatcaa tgaccgccag ccgccggtac atatcgtcgg
      781 cgtcgttgac ctcgcgcggc gaccagaatc gctcggcaat cgccgccgtg cgcgaccaga
      841 tgcgcgaatc aatcgtctcg ggcgaaaccc attcgctcca catcgtcgcc tcgccgccga
      901 gcacgcgctt ttgttcttcc gccgtcagag tcgtatcggc gggcagcggg tcgaccgcgt
      961 aatgctgcga cgccggaaaa acgaggtcga tataatagcc gttcgacaaa acgctctgat
     1021 agccctgccg cgccgcttcg gctaaagcct tctgcccgcg ccacgaatga ataacgatgt
     1081 ctttcggcaa atcgggctga aaaatttcgt cccagcccat cataattttg ccgttgtcct
     1141 gcaataattc gagcagccgc cggttaaaat aagtttgcag cgcgtggttg tccctgatgc
     1201 ctttttcctt cataaaggct tggattttcg ggttttcggt ccactgttta cctttatttt
     1261 cgtcgccgcc gatgtgcata tacgcgtcgg ggaaaagcgc cgacatttct ttaaaaaata
     1321 ctttcagaag cttgtatgtc ttctcgtttg tcgggtcgag cgtcgggtcg aaaattcccg
     1381 gtcggcgctc gattttatac ggtcccggcg cgctgccgat ttcgggatga ctgacgagcc
     1441 acgccgtcgc gtgaccgggc atatcgaatt ccggcatcac gcggatgccg cgctcggcgg
     1501 cgtaatcgat gatttcgcgg atttcgtttt gcgtgtaaaa aagcccgtcg gagccgagcg
     1561 cgtgcagttc cggcattttt ttactctcga ctcgaaagcc ctggtcttcg gtcagatgcc
     1621 agtgcaaaac gttcaatttt acagccgcca tcgcgtccag attgcgtctt aaaacttcca
     1681 tcggctggta atgccgcgcc gaatcaatca tcaaaccgcg ccacgggaaa cgcgggcggt
     1741 cggagatgga aaccgccgga aaataaaatc cgtttttatc gccttccaga agctgtagaa
     1801 aagtttccag cccgcgcatc gcgccgacgg ttgtcggcgc ggtcaatctc gcctgatttt
     1861 cggaaatttc gagattgtag gattcgtcgt ccccgagttt cggaatcgcg tttccggttg
     1921 attgggtttc gattaaaaga ttcgcgcccg ccgcatcatt cgataattcg cgcgaaaatt
     1981 ccagaaccgt gcgcccttcg agccgccgca tcacgcggaa aatatatttt ttcaggcgct
     2041 cgtcggtctg ccctttgacg gcgaccgcga aagctttcgt gacgggcaat ctgccggttt
     2101 tccagctcac gctcaacggc acgggcatca aattgcgccg cgccgactgc aaggcgtttt
     2161 cctgcgccga aacaactgaa agcgccgacg aaagcagcgc gaaaattaaa aaaattccgg
     2221 ttaaaaactt ctgcataaaa tcaaaattcg tgaatccctg cgttttttat tttaaaggct
     2281 tttgtaaatt atacgactca aacgcctttc gctcacaatc ggaaattttt ggcgtccctt
     2341 tttcaaacat attaaagccg ccgtcgcatt cgcgccaggc gttcggaata tttaattttt
     2401 ccagtgccga acagagggcg cgggctttcg cggcagcggt cttccgtcgt tgaagaatct
     2461 cgattgataa aatagaatgt gttaacactt ttaaatcttt atttcaaagg acaattaaat
     2521 ttttaatgcg gaaagtaacg ctgattttag cagacataag gctaacccgt aacttttgcc
     2581 cgccgcgaca aactttttgt tcagtcccgg gacgaagcat ttagataaga tgcagcgagc
     2641 aacagacaac gaacgacctg cggacgcatt gctggaaatg cgttcaatcg gcaaggagtt
     2701 tcccggcgtg cgggctctgg acggcgtcac gcttgacctt tacgaaggcg aatttcacgc
     2761 gctcgtcggc gaaaacggcg ctggaaaatc cactttgatg aaagttcttt cgggcgtcta
     2821 tccggcggga agctacgacg gcgagatttt gattgccggc gaggtgaaac agtttaagac
     2881 catccgcgac gccgaaaacg cgggcatcgc catcatcttt caggagcttt cgctggttaa
     2941 ggaattgacc gtcggcgaaa acatttttct gggacgcgag ccgagccggt tcggcgtcat
     3001 aaattgggct gagctttatc agaaagccgg aacgcttttg caggaattgc atctggcgat
     3061 tgacccgcgc acgccggtcg gaaatctcgg catcggacag cagcagctgg tcgaaatcgc
     3121 caaagctctg tcgcagaaag cgaaaattct ggttttggac gagccgacgg cggctctgac
     3181 cgaagcggaa gtcaaaacgc tgtttgcgat tctcgaaaag cttcgggcgc gcggcgtcgg
     3241 aatgatttat atctcgcaca agctcggcga agttttccgt atgagcgatc gcatcacggt
     3301 tttgcgcgac ggtaaaacgg tcggaacaaa cgagacggca aatttaaata tcgaaaaagt
     3361 catcgctttg atggtcgggc gcgaagtcgg cgatattttc ccgaaaacca atcaccaatt
     3421 cggcgaaatc gcgttggaga taaaaaactt atccgccttc gacctcgaca atccgaataa
     3481 aaaactggtc gatgatgttt cgtttaatgt tcgcaaaggc gaggttctgg gcatcgcggg
     3541 tttaatggga gcgggacgca gcgagctttt aatgtcgatt ttcggcggct ggcagggcaa
     3601 aagccggggc gagatttacg tcgaaggtcg gcagattttc atcggcgcgc cctcggacgc
     3661 aattcgccac ggcatcggat tcgttaccga agaccgaaag cgtttcggat tgcttctgga
     3721 acagacgatt ttagacaata tgacgctcgc cggattaaaa caaatgtccg gcaaaatgct
     3781 gacgcatcgc tcgcgcgaga cggtcgcggc gagcggcgcg atgcagtctt taagagtcaa
     3841 agccggctcg cccttaaccg tcgcgggaac gctttccggc ggaaaccagc aaaaggtcgt
     3901 gctgggaaaa tggcttttga cgaacccgaa aattttgttt ctcgacgagc cgacgcgcgg
     3961 catcgacgtc ggcgccaaac aggaaattta cacggaaatc aacaagctgg cgcgggaagg
     4021 cttggcgatt gtgctggttt cgtccgagct gccggaggtt ctgggtttgt cggacagagt
     4081 tattgtttta cacgaaggca gattgaccgg cgaatttacg cgcaccgaag cgaccgccga
     4141 aaaagtgatg aaggcggcga ccggaaatgt tcagcaaact tcgccgccaa gacctgaaga
     4201 cgaaattaac cgggaattat caggataaga tgtggtaaat aaaatctatt ttaccgctct
     4261 gcgcgcgtcc tgttatccgc ggttaattat tttctctgtt tcccgacggt gaacgaattt
     4321 taaaaatgaa cgaagaaaca aaaataagcg gcttgaaatt tcaaacgtcc tcgcttcgcg
     4381 catacacgat gctcggcgcg ttgattttga tttggctttt gttttactgg cagacgggcg
     4441 gaacttttct gacgccgcgc aatttgtcga acctgatgct gcaaacttcc gtgaccggca
     4501 ttttggcggt cgggatgctg atggttatct tagccgggca gattgatttg tcggtcggtt
     4561 cggttctggg tctggcgggc ggcgtggcgg cgatttcgct gactaattgg gattacgggt
     4621 tagccggttc gattttgctg gcgattgccg tcggcgtagc tatcggttta attcacggga
     4681 cgctgacggc ttatttcaat attccggcgt ttatcgtcac gctcggcggg cttctggcgt
     4741 ggcgcggcgc gataaaaggc atctccagga gcgaaaccgt tcccatcgcc gacccgactt
     4801 ttaagttgat cgggcagggc aacattaccg ccaattccgg ctgggtttta gccggaatcg
     4861 ggattctgct gattttgttt ctggcatacc ggcgggcgca ggcgcagaag gattacgggc
     4921 tgggcgaggc aaattacggc ggcgagatgc tgaaaacggt tttgccgatc ggcgtcatta
     4981 tcggatttat ctatgtgatg aactcctcgg acggcgtgcc gattccggtt ctgatttttc
     5041 tcggcgtggc gattttcggc gtgtttatta ccaattcgac caccttcggt cgttatcttt
     5101 acgcgatcgg cggaaactcg gacgccgcgc ggctttcggg catta
//
LOCUS       DASWNE010000636        10172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_372965, whole genome shotgun sequence.
ACCESSION   DASWNE010000636 DASWNE010000000
VERSION     DASWNE010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10172
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372965"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..786
                     /locus_tag="VGB68_12455"
     CDS             64..786
                     /locus_tag="VGB68_12455"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0050096.1"
                     /translation="MITDDYQHPATYVSTGNPAVDFRYSVEVFEADISNYLPQNKETP
                     ILDVGCGWGQLLWWLREKGYTSVQGIDIGRAQEEHGNSIGLDIICVEDSTDFLENLDS
                     KYELIIMNHIIEHMPAAEGIKMLKAVYKALSPSGRVIVQTPNLCAIGANCGRYIEISH
                     VTGYTESSLHQIVNLAGFADIELFGSKTAFRLAPRRLVLLLLQFISRAIWRIMLLAEW
                     GTDAPRIIQRNLYATGIKPARE"
     gene            864..1451
                     /locus_tag="VGB68_12460"
     CDS             864..1451
                     /locus_tag="VGB68_12460"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HEY0050097.1"
                     /translation="MRNLRKRLTPENEHTALWMAQNKRYSAFEIGVGTYGTPNIDFHN
                     AGATLKIGNYCSIAPRVTILLGGEHHHDWVSNYPFSLLHDEARHLTGYPLTKGDVLIG
                     NDVWIGYEALILSGVKIGDGAVIAARSVVSRDIEPYSIVGGTPAKHLRYRFSPEIIDE
                     LLKIGWWNWSFEEIRAAWHLIQSPQVEEFVSKYKN"
     gene            1483..2619
                     /locus_tag="VGB68_12465"
     CDS             1483..2619
                     /locus_tag="VGB68_12465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0050098.1"
                     /translation="MRICYFADGRSVHTKRWISFFAGRGYEMHLISFKPMDERQTAEM
                     EEIGVRCHGHVGDFHLKKFWLTWKDVQKVRRVLKKEKIDILHSHFLGTNTWFATASLF
                     RPHIITVMGGDVKGETWQPNGNIQERVLTPMALKKADKVTAWSKKIGEIVSKYRAGED
                     EIEVIHGGIHLENFTPAPKPKRLLEKLGIPENANIVFSPRLVRRLYNIDQIARAAGEV
                     CDELPNTYFLIALPSMILDEEYTAEIKQIFAENSARSTVRYLTDIPHHEIADYFRLAD
                     VTVCVPATDGTPMSVLESMACGAPTVIGNLPDYDAEYFENEKTVLTAEVDNPQSIAKA
                     ILRYLNEPDFAARIAAEARRRVLETGDYEFQMNKMEQVYRRILK"
     gene            2619..3290
                     /locus_tag="VGB68_12470"
     CDS             2619..3290
                     /locus_tag="VGB68_12470"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0050099.1"
                     /translation="MANLVKKIRATIAAPPTPYLVTGRTQNRLQSLIDSMPPDAVILN
                     VGAGFTNLGERVINLDIFDSGTTDVIASSLEMPFADESGDLLILQGVLEHVVSADKTL
                     EECFRVMKKGGLFYTEMPFLQPYHATPIDLRRTTLPGITELCLPLEEVEKGIHTGPAS
                     TITWIVRELIAGLLAGGNQRIFPYINSLVGWVVFPFKYLDYWLEKKPYLHCIASAVYY
                     IGRKT"
     gene            3294..4166
                     /locus_tag="VGB68_12475"
     CDS             3294..4166
                     /locus_tag="VGB68_12475"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0050100.1"
                     /translation="MSSYCPLCNTTESEASWLGATRYREKKYDYLQCAECDSLYCSPM
                     PDEETLALMYGDDYQQFLSVESTHSGDDGIRQVIEYLKTLEKGVFFDYACGAGVLLSE
                     VAKIGWDVLGLELNVATAEKYSRLHNIKIISDLKDLPENFRADVIHLGDVLEHLTDVN
                     SQFPSILNLLKENGLLIAQGPLEANLNLFFLGVKMTRILRRAPISEMPPYHVSLATVE
                     GQRKFFNRFKLNEMVFDVFETSHPAPERIALSDLKDVRKSTFFLLRKVSQTVSAVFPK
                     TMGNRYFYVGSKGK"
     gene            4144..5340
                     /locus_tag="VGB68_12480"
     CDS             4144..5340
                     /locus_tag="VGB68_12480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0050101.1"
                     /translation="MSEARANNQNRKLKVLHLIEGLGSGGAERLLYTNLKHFDRARLE
                     NEVVTVFSAADYWKQPILDLDVPVSTLDCRSLRDIMTGVRRLRKEIARFQPHLIHTHL
                     WTANIVGRIAGFLTGTPVISSIHNPEYEPEVLTDTAHLNSKKLFVARSLDKWTARVAC
                     RKMLAVSRYVKASASLHLDFPPEKIEVLYNPVDVSDLCSGSVRTRAGVLNELNLPADS
                     LILLNVARVAPQKGLLYAIRSLAEIRRKFPETHLISVGGKDDATWLARLEEEIEKLGL
                     KNHVHLLGARRDVKDFLYNCDLFVFPSLFEGLGIALTEAMAVGCACVASDIKPLDEFV
                     ENEVDGMFFPPRNPAALARAAVNLLENEKKREAIGAAAREKALSLFQPQPAADKLTEI
                     YFSMIK"
     gene            5499..6752
                     /locus_tag="VGB68_12485"
     CDS             5499..6752
                     /locus_tag="VGB68_12485"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0050102.1"
                     /translation="MDIKTLYISYFGIREPLVQTQVVAYLREIADGGVTVHLVTFEPN
                     FKQSWTPEQVEEERKKLAAQGIAWHCLAYHKSPSVPATLYDISVGARFIRKLIRREGI
                     NVLHARGHIPVLMAAIANAGNNCQIIFDIRGLVAEEYADAGIFPHGSPPFRAIKWIER
                     LGLKKAAQIVVLTNKMRDYLVEERAVRPEIIEVIPCCVDFSRIENAELKLPPTQRFEL
                     IYAGSVSGLYLLEEMCAFFLELKKRKPDAFFRILTKHPAEETAKAFQKFGIGENDYEV
                     LSVKPSEVGTYIKQASLAISFRKSTFAQIAASPTKIPEYLAVGVAVVSNAGIGDTDAL
                     IENEKVGVTVSDLSPAGYAQAADAALELLRDESLYERCVRVAHDGFDLKSVGRNGYLN
                     VYKRLSSARAVEADGLSAVPTKERI"
     gene            7103..8239
                     /locus_tag="VGB68_12490"
     CDS             7103..8239
                     /locus_tag="VGB68_12490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0050103.1"
                     /translation="MKLLAIVPSLYDISPGQRYRIEQWEPILREKGVEITYSPFETDE
                     LRGVLYQPGRLAEKMKFVMKSINRRREDLRSVRDFDAVYVFREAALLGPAYFERLIKR
                     KGVPMIFDFDDAVFVSYKSPSNGYLSYLKFPGKTATICRLSTHIMAGNPYLADYARQY
                     NPAVTIVPTTIDTDKYEFSANKPANDVPVIGWSGSHSTVQHLDTLRETLQDLARVAKF
                     RLRVIGTANYEIEGVDVEAMPWRSETETSDLQPIDIGVMPLPDDQWSKGKCGLKALQY
                     MALGVPTMCSPVGVNSDIIQDGENGFLAGTKDEWIEKLKRLLASVELRRRLGAAGRRT
                     VEEKYSARVQAPRVFQILASVTSAKAATKKSLEAEKQEEKSYSI"
     gene            8264..9391
                     /locus_tag="VGB68_12495"
     CDS             8264..9391
                     /locus_tag="VGB68_12495"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0050104.1"
                     /translation="MEKFNILVTGGAGFIGSHLVDALVEKGHRVRILDSLVEQVHGGQ
                     VPEHLNKDAEFIQADMNDAEAVSRALDGIDVVYHKAAEVGVGQSMYEIVRYVKANDLG
                     TAVLLEEMIKRPKQFRKLVVASSMSIYGEGAYTCEGCNETVYPYLRENEQLEAQDWDF
                     KCEKCGDAVLKPIGTSESKPLFPTSVYAVSKQDQEQYCICVGRAYKIPTVAFRYFNVY
                     GTRQALSNPYTGVCAIFSSRLLNNESPMIFEDGGQTRDFIHVSDIVQANLLALETDKA
                     DYQPVNVGTGRPISVKEISQLLAKGLGKDIEPQIVGKYREGDIRHCVGDITRARQLLG
                     YEPKVNLEEGLMNLLEWVKDQKPADRVATATAELASRSLVR"
     gene            9398..>10172
                     /locus_tag="VGB68_12500"
     CDS             9398..>10172
                     /locus_tag="VGB68_12500"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEY0050105.1"
                     /translation="MTLRKTLKTIYRRVPFKRQLFSVVKTFVTPSERVYKHLHFQDDF
                     RVCVDEKHSFRMRHGGLELENTLFWVGPSGGWERNSMSIWIKLVKNAEVIFDIGANTG
                     YYSLVAKALNSKAKIYAFEPIAESFHNLEYNNRINDFDIVCFDCAASNVDGEAKIFAA
                     PDDFYAVTLNKDYLDKKDISSVPTTIKVARLDTLIEEFKIKKIDLIKLDVETYEAEVL
                     EGLGKYLDEFRPTLLIEILNDDVGHRIEALIKGKDYLYFN"
BASE COUNT         2589 a         2482 c         2711 g         2390 t
ORIGIN      
        1 caataattcc gggaaagtat attattgttt tttttcctta ttaagaagcg ttagcgttaa
       61 tttatgatta cagatgacta ccaacatcct gctacttatg tctcaactgg taatcctgcc
      121 gtagattttc gatattccgt cgaggtcttt gaagccgata taagtaatta tttacctcaa
      181 aacaaggaaa ctcctatttt agatgtcggc tgcggttggg gacagctttt gtggtggtta
      241 cgagaaaaag gatacacaag cgtccagggc atcgatatcg gaagggctca ggaagagcac
      301 ggcaactcga tagggctcga tattatttgc gtggaagatt cgaccgattt tttggaaaat
      361 ctggattcaa agtatgaact gatcataatg aatcatataa tcgaacatat gcccgccgcc
      421 gaaggaatta aaatgctgaa agccgtttat aaagcattaa gtccgagcgg gcgagtcatc
      481 gtgcaaacgc ctaatctttg tgctatcggg gctaattgcg ggcgttatat tgaaataagc
      541 catgtcaccg gctatacgga gagcagcttg caccagatag taaatttagc cggttttgcc
      601 gacatagagc tttttggaag caagaccgcc tttcgattag ctccgcgccg gctggtatta
      661 ttattattgc aatttatttc aagagcaatc tggcggataa tgctgctggc ggaatgggga
      721 acggatgccc ccaggattat tcaaagaaac ctttacgcga caggcattaa accggctcgg
      781 gaataaagcg ggcgcggctt ttgagccggt aaataatatg ttctctgcta aaagcagaat
      841 aagatcgtat gctgagaaaa gttttgcgga accttcgaaa acgcctgacg cccgaaaatg
      901 aacacacggc gctgtggatg gcgcaaaaca aacgctacag cgcatttgaa atcggcgtcg
      961 gcacgtacgg aacgccgaat atcgattttc ataacgccgg agcgactttg aaaatcggaa
     1021 attactgctc gattgcgccg cgcgtgacta tcctgctcgg cggcgagcac caccacgatt
     1081 gggtgagcaa ttatcctttc agcttattgc acgacgaagc gcggcatttg accggttatc
     1141 ctctgaccaa aggcgacgtc ctgataggca acgacgtctg gataggctac gaggcattga
     1201 ttttatcggg cgtgaaaatc ggcgacggcg cggtcatcgc ggcgcgcagc gtggtttcga
     1261 gagatattga accttattcg atagtcggcg gcacgcccgc caaacatctg cgttatcgtt
     1321 tttcgccgga gataatcgac gagctgctca aaatcggctg gtggaactgg tcttttgaag
     1381 aaattcgcgc cgcctggcac ctgattcaat ccccacaggt tgaagaattc gtctcaaaat
     1441 ataagaattg agttttaaca agcgggagaa tactcataaa aaatgcgaat ttgttatttt
     1501 gccgacgggc gttcggttca caccaaacgc tggataagct tttttgccgg gcgcggctac
     1561 gagatgcatc tgatttcatt caagccgatg gacgagcggc aaacggctga aatggaagaa
     1621 atcggcgtcc gctgtcacgg tcacgtcggc gattttcatt tgaaaaaatt ctggctgaca
     1681 tggaaagacg tgcaaaaagt gcggcgcgtt ttgaaaaaag aaaaaatcga cattctgcat
     1741 tcgcattttc tcggcacgaa cacctggttt gccaccgcat cgctgtttcg cccgcatatt
     1801 attaccgtga tgggcggcga cgtcaaaggc gaaacgtggc agccgaacgg aaatattcag
     1861 gagcgggtgc tgacgccgat ggctttgaaa aaagctgata aagttaccgc gtggtcaaaa
     1921 aaaatcggcg aaatcgtcag taaatatcgc gcgggcgaag acgaaatcga agttattcac
     1981 ggcggcattc atctggaaaa ttttacgccc gcgccgaagc cgaaacgtct tttggaaaaa
     2041 ttggggattc ccgaaaacgc caatatagtt ttcagcccgc gcctggttcg ccgtttgtat
     2101 aacatcgacc agatagctcg cgcggcaggc gaggtttgtg acgaattgcc gaacacttat
     2161 tttttgattg ctctgccttc gatgattctg gacgaagaat acacagcaga gataaaacaa
     2221 attttcgccg aaaattcggc gcgctcgacc gttcgctatt taaccgatat tccgcaccac
     2281 gaaatcgccg attattttcg actggcggac gttacggttt gcgttccggc aaccgacggc
     2341 acgccgatga gcgttttgga atcgatggcg tgcggcgcgc cgaccgtcat tggcaatctg
     2401 ccggattatg acgcggagta ttttgaaaac gaaaagactg ttttgaccgc cgaagtcgat
     2461 aatccgcaat caatcgccaa agcgattttg cgctatttaa acgagcccga tttcgccgct
     2521 cgaatcgctg ccgaagcgcg ccgcagagtt ttggagacgg gcgattatga atttcagatg
     2581 aataaaatgg agcaggttta ccggcgcatt cttaaataat ggcaaatttg gttaaaaaaa
     2641 ttcgtgccac catcgctgcg ccgccgacac cgtatctggt taccgggcgg acgcaaaatc
     2701 ggctacaatc gttgattgat tcgatgccgc ccgacgcggt tattttgaac gtcggcgcgg
     2761 gattcacaaa tttaggcgag cgcgttatca atctcgatat tttcgattcg ggcacgaccg
     2821 acgttatcgc ttcgagcctg gaaatgccgt ttgccgacga aagcggcgat ttgctgattc
     2881 tgcagggcgt tctggaacac gtcgtcagcg ccgataaaac tctggaagaa tgctttcgcg
     2941 tgatgaaaaa aggcgggctg ttttatacgg aaatgccgtt tttgcagcct taccacgcga
     3001 cgccgattga tttgcgccga acgactctgc ccggcatcac cgagctttgc ctgccgctcg
     3061 aagaagtcga aaaaggcatt cacacgggac cggcttcgac gattacctgg attgtgcgcg
     3121 agcttatcgc cggacttctg gcgggcggca accagcggat ttttccgtat attaacagtc
     3181 tggtcggctg ggttgttttt cccttcaaat atctggatta ctggctggag aaaaagcctt
     3241 atctgcactg catcgccagc gccgtttatt atatcggaag gaaaacgtaa cggatgagtt
     3301 cttactgtcc tctttgcaac actaccgaat ccgaagcctc ctggctcggc gcgacgcgct
     3361 atcgtgaaaa aaaatacgat tacctgcaat gcgccgaatg cgattccctg tattgctcgc
     3421 cgatgccgga cgaagagacg ctggcgctga tgtacggcga cgattaccag caatttctct
     3481 cggtcgaatc gactcacagc ggcgacgacg gaatccggca ggttatcgaa tatctgaaga
     3541 ctctggaaaa aggcgttttc ttcgattacg cgtgcggcgc gggcgtgctt ctcagcgaag
     3601 tcgcgaaaat cggctgggac gttctggggt tagagctgaa cgtggcgacg gcggaaaaat
     3661 acagccgcct ccataatata aaaatcatct cggatttaaa ggatttgccg gaaaattttc
     3721 gcgccgacgt gattcatctg ggcgacgttc tggaacatct gacggacgtc aacagccagt
     3781 ttccttcgat tctcaattta ctgaaagaaa acggtttact aatcgcgcaa ggtccgctcg
     3841 aagccaacct caaccttttc tttctcggcg tcaagatgac ccgaatcctg cgccgcgcgc
     3901 cgatttccga gatgccgcct tatcacgtca gcctcgccac ggtcgaaggt cagcgaaaat
     3961 tttttaaccg tttcaaacta aatgagatgg tcttcgacgt tttcgaaact tcgcatccgg
     4021 cgcccgagcg catcgcgctg tccgatttga aagacgttcg aaaaagcacg tttttcctgc
     4081 tccgcaaagt ttcgcagacg gtttccgccg tttttcccaa gacgatggga aatcgttatt
     4141 tttatgtcgg aagcaagggc aaataatcaa aaccgcaagc tgaaagttct gcacctgatt
     4201 gaaggactcg gctcgggcgg agccgagcgg ctgctttaca cgaatctcaa acacttcgac
     4261 cgcgcgcggc tggaaaacga agtcgtcacc gttttttccg ccgcagatta ctggaaacag
     4321 ccgattctcg acctcgacgt tccggtctcg acgctcgatt gccggagcct gcgcgacatt
     4381 atgacgggcg ttcgccgcct gcgaaaagaa attgcgcgat ttcagccgca tttgattcac
     4441 acgcatctgt ggacggcaaa catcgtcggc agaatcgccg gttttttgac cggaactccg
     4501 gtcatcagct cgattcataa tcccgaatac gagccggaag ttctgaccga taccgctcat
     4561 ctgaattcaa aaaaactgtt cgtcgcccgc agtctggaca aatggacggc gcgcgtcgcg
     4621 tgccggaaaa tgctcgccgt cagccgctat gtcaaggcaa gcgccagcct tcatctcgat
     4681 ttcccgccgg aaaaaatcga agtgctttac aatccggtcg acgtttcgga tttgtgttca
     4741 ggctccgtcc ggacgcgcgc cggcgtttta aacgaactaa atttaccggc ggattcgctt
     4801 atacttttaa acgtcgcgcg cgtcgcgccg cagaaaggct tgctttacgc gattcggtcg
     4861 ctggcggaaa ttcgccgaaa gtttcccgaa acgcacctga tttccgtcgg cggcaaagac
     4921 gacgcgacct ggctggcgcg gctcgaagag gaaatcgaaa aactcggctt gaaaaatcac
     4981 gttcacctgc tcggcgcgcg gcgcgacgtc aaagattttt tgtataattg cgatttgttc
     5041 gttttccctt cgcttttcga aggcttggga atcgcgctga ccgaggcgat ggcggtcggc
     5101 tgcgcctgtg tggcaagcga tataaaacct ttggacgagt ttgtcgaaaa cgaagttgac
     5161 gggatgtttt tcccgccgag aaatccggcg gcgctggcgc gagccgccgt caatctactg
     5221 gaaaacgaaa aaaaacgcga agcgatcggc gcagccgcgc gagaaaaggc tttgagtctt
     5281 tttcaaccac agcccgcggc tgataaacta accgagatat atttttcgat gattaagtga
     5341 taatttaagg caaaagtaaa aagggcaaaa ggcagcagtg aaaacgaaga attgaaatta
     5401 aaacaggatt ttctctgtta ccgttcgttt cgcgcacgct ggttttgtct ctcgccgttt
     5461 tacttttcgc cttctaaaat cgcttctggt ttgaatttgt ggacataaaa accttataca
     5521 tctcatattt cggaattcgc gagccgctcg tgcagacgca ggtcgtcgct tacctgcgcg
     5581 agattgcgga cggcggcgtg acggttcatc tcgtcacctt cgagccgaat ttcaagcaaa
     5641 gctggacgcc ggagcaggtt gaagaagaaa gaaaaaaact ggcggcgcag ggcatcgcgt
     5701 ggcattgcct ggcttatcat aaaagccctt ccgtgccggc gacgctctac gacatttcgg
     5761 tcggcgcgcg gtttatcaga aaactcatca ggcgcgaagg aatcaacgtg ctgcacgcgc
     5821 gcgggcacat tccggtcttg atggcggcaa tcgccaacgc cggaaacaat tgtcaaatca
     5881 tcttcgacat tcgcggtctg gtcgccgaag aatacgccga cgcgggaatt ttcccgcacg
     5941 gctcgccgcc ttttcgagcg attaaatgga ttgagagact gggattgaaa aaagccgcgc
     6001 aaatcgtggt tttgaccaat aaaatgcgcg attacctggt cgaagagcgc gcggtcagac
     6061 cggaaatcat cgaggtcatt ccctgctgcg tggatttttc gcgaatcgaa aacgccgagc
     6121 tcaagctgcc gccgacgcaa agattcgagc tgatttatgc cggttcggtc agcggtttgt
     6181 atctgctgga agaaatgtgc gctttttttc tggagctcaa aaaacgaaag cccgacgctt
     6241 tttttcgcat cctgacgaaa catccggcgg aagaaacggc gaaagctttt caaaaattcg
     6301 gcatcggcga aaacgattac gaggttttgt ccgtcaagcc ttcggaagtc ggaacttata
     6361 tcaaacaggc gagcctggcg atttccttta gaaaaagcac tttcgcgcag attgccgctt
     6421 cgccgacgaa gattcccgaa tatctggcgg tcggcgtcgc ggtcgtatcg aacgccggca
     6481 taggcgacac ggacgcgctg attgaaaacg aaaaagtcgg cgtgaccgtc agcgaccttt
     6541 cgcccgcggg ctacgcgcag gcggcagacg ccgcgctgga gcttttacgc gacgaatcgc
     6601 tttatgaaag atgcgttcgc gtggcgcacg acggtttcga tttaaaatcg gtcggcagaa
     6661 acggttatct taatgtttat aaaaggttat cgagcgcgcg cgccgtcgaa gccgacggat
     6721 tatccgccgt gcccacaaag gagcggattt agatttatct ttttgtataa tttgaagtaa
     6781 tcgtcttaac gctgtaaaat tcgttgaagc taatatcgca cattttatag aaatagctta
     6841 tagttttaag tttgcgtcat taaaaattat ttattaggta tgtatggtag gtaacagagt
     6901 tttaacgccg tttttgataa gttacgggaa attataagta atcgagtgag attcagcaga
     6961 gagagacgta gcagcaggca gaggcaaatc ttcccccttt aatcgcttca tttcttctac
     7021 ggcgcggagc cgggaaaact cgctaaggaa ttctctaatc attgccgaat aacggacaaa
     7081 ctccttgccc cgcattttta gtatgaaact tttagccatc gttccgagtc tttacgatat
     7141 atcgccgggt cagcgctacc gcatcgaaca atgggagccg attttgcgcg aaaagggcgt
     7201 ggaaatcact tattcgcctt ttgaaaccga cgaattgcgc ggagttttat atcagcccgg
     7261 acgcctcgcg gaaaagatga aattcgtgat gaaaagcatc aatcggcggc gcgaggattt
     7321 gcgcagcgtc cgcgatttcg acgcggttta cgtttttcgc gaagccgcgc ttctgggtcc
     7381 cgcgtatttc gagagattga ttaagcgcaa gggcgtgccg atgattttcg atttcgacga
     7441 cgcggttttc gtttcctaca aaagtccttc caacggttat ctaagctacc tgaaatttcc
     7501 gggcaaaacg gcgacgattt gccgcctgtc gacgcacata atggcgggca atccttattt
     7561 ggctgattac gcgcggcagt ataatccggc ggtgacgatt gtgccgacga cgattgacac
     7621 cgacaaatac gaattttccg caaacaaacc ggcaaacgat gttccggtta tcggctggag
     7681 cggcagccac agcacggttc agcatctgga cacgctgcgc gagaccttgc aggatttggc
     7741 gcgcgtggcg aagtttcgcc tgcgcgttat cggcacggcg aattatgaga ttgaaggcgt
     7801 cgacgtcgag gcgatgccct ggcgctcgga aacggaaacc agcgatttac agccgatcga
     7861 catcggcgtg atgccgctgc cggacgacca gtggagcaaa ggcaaatgcg ggctcaaagc
     7921 cctgcagtat atggctctcg gcgttccgac gatgtgctcg ccggtcggcg ttaattccga
     7981 tattatacag gacggcgaaa acggttttct ggctggcacg aaagacgaat ggattgaaaa
     8041 actaaaacgc ctgctcgcgt ccgtcgaact gcggcggcgg ctcggcgctg ccggacgccg
     8101 gacggtcgaa gaaaaatatt ccgcgcgcgt tcaggcgccg cgcgtatttc aaatcttagc
     8161 gtcggtcacg agcgcaaaag cggcgacgaa aaaatcgctc gaagcggaaa aacaggaaga
     8221 aaagtcatac agcatctgat tttgggggaa acaggcaaaa ggaatggaaa agtttaacat
     8281 cttggtaacg ggcggcgcgg gttttatcgg gtcgcatttg gttgacgcgc tggtggaaaa
     8341 agggcatcgc gtaagaattc tggattcgct ggtcgaacag gttcacggcg ggcaagtgcc
     8401 ggaacatttg aacaaggacg ccgaatttat acaggcggat atgaacgacg cggaagccgt
     8461 cagccgcgcc ctcgatggaa tcgacgtcgt ttatcacaag gcggcggaag tgggcgtcgg
     8521 gcagtcgatg tatgagatcg ttcgctacgt caaagcaaac gatctgggca cggcggtttt
     8581 actggaagaa atgattaagc gcccgaaaca gtttcgcaag ctcgtcgtcg cttcgtcgat
     8641 gtcgatttac ggcgaaggcg catacacctg cgaaggctgc aacgaaaccg tctatccgta
     8701 tttgcgcgaa aacgagcagc tcgaagcgca ggattgggat ttcaaatgcg aaaaatgcgg
     8761 cgacgccgtc ttaaagccga tcgggacgag cgaaagcaaa ccgcttttcc cgacttccgt
     8821 ctacgccgtc agcaaacagg accaggaaca atactgcatc tgcgtcggtc gcgcttataa
     8881 aattccgacc gtcgctttcc ggtatttcaa cgtttacggc acgcgtcagg ctttgtcgaa
     8941 tccgtacacg ggcgtctgcg cgattttttc gtcgcggctg ctgaacaacg aaagcccgat
     9001 gattttcgaa gacggcgggc agacgcgcga tttcatacac gtcagcgaca tcgtgcaggc
     9061 aaatcttttg gcgctcgaaa ccgacaaagc cgattaccag ccggtcaacg tcggcacggg
     9121 acgaccgatt tcggttaagg aaatctcgca gctgctcgcc aaaggcttgg gcaaagacat
     9181 cgagccgcaa atcgtcggca aataccgcga aggcgatatt cgtcactgcg tcggcgacat
     9241 cacgcgcgcg agacaattat tgggctacga gccgaaagta aatctcgaag aaggcttgat
     9301 gaatcttctt gaatgggtca aagaccagaa accggctgac cgtgtcgcga cggcgacggc
     9361 ggagctggcg tcgcgctcgc tggtcagata aattttaatg acactaagga aaaccctcaa
     9421 aacaatctac cggcgagttc cgtttaaacg gcagcttttc agcgtcgtta aaactttcgt
     9481 cacgcccagc gagcgcgtct ataaacatct tcattttcag gacgattttc gcgtctgcgt
     9541 cgatgaaaag cactcgttcc ggatgcggca cggcggcttg gagctggaaa atactttgtt
     9601 ttgggtcggt ccgagcggcg gctgggaacg aaactcgatg tcaatctgga taaaactggt
     9661 caaaaacgcc gaggtgattt tcgacatcgg cgcgaatacc ggttattatt cgctggtcgc
     9721 gaaagctctg aattcgaagg cgaaaattta cgctttcgag ccgattgccg aaagctttca
     9781 taatctggaa tacaacaacc ggattaacga tttcgacatt gtctgctttg actgcgcggc
     9841 gtcgaacgtc gacggcgaag cgaaaatttt tgccgcgccg gacgattttt acgccgtcac
     9901 tttaaacaag gattatctgg ataaaaaaga catttccagc gtgccgacga cgattaaagt
     9961 cgcccgtcta gacactttga ttgaagaatt caaaatcaaa aagatagatt taatcaagct
    10021 cgacgtggaa acttacgagg cggaagttct ggaaggattg ggcaaatacc tggacgaatt
    10081 tcgacccact ttgctgattg agattttaaa tgacgacgtc gggcaccgca tcgaagcgtt
    10141 gataaaagga aaagattact tatactttaa ta
//
LOCUS       DASWNE010000637         2640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_22263, whole genome shotgun sequence.
ACCESSION   DASWNE010000637 DASWNE010000000
VERSION     DASWNE010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22263"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VGB68_12505"
     CDS             complement(<1..364)
                     /locus_tag="VGB68_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050106.1"
                     /translation="MSENRLENLLQPLAITFFLFRKSPVFDKYAETAKQKDRTTDFSR
                     IRCPLCRWQPQASSRWCCSDAAGYPHFFHNGCGTVWNTFETRGRCPGCGHQWSRTDCL
                     RCRGCSPHEDWYEDETATT"
     gene            complement(379..1437)
                     /locus_tag="VGB68_12510"
     CDS             complement(379..1437)
                     /locus_tag="VGB68_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050107.1"
                     /translation="MKISTKSRLETNLKKHLILLSLFALMFYSSAAAQWQKQTIETRA
                     SLRGLAVVSEKIIWASGTGGSFLKTTDGGKNWTVGKVPGAEKLDFRDVEAFDANTAYL
                     LSIGEGENSRIYKTTDGGATWKMQFKNANPKAFFDAIAFWDENNGIAMSDPVDGKYLL
                     MTTSDGETWKPLGAEKMANAKEGEAAFAASGTCLITSGKSDVFLVSGGNDARVFRSNN
                     RGLSWFVSETPIVRGTAGSGIFSIAMFDSKRGAIVGGNYEKPNETTNNLAFTTDGGKT
                     WKPGKGLTGYRSGAAYIDKKTMVAVGASGSDLSVDGGKTWKNLDKENYNSVQAKGKRA
                     VWAVGANGLVARFIAERL"
     gene            complement(1463..1924)
                     /locus_tag="VGB68_12515"
     CDS             complement(1463..1924)
                     /locus_tag="VGB68_12515"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEY0050108.1"
                     /translation="MSIERNKNIVRRFYEELWNERKLATADEIFAPECVTHQLESGAA
                     TVAAPRSAEAIKEHIGEWLGAFPDLHFTVERMVAENDLVITYATMHGTHTGNWHGIPA
                     TGKRVGIRMTAAHRIADEKIAADWVLVERLGFFQQLGLLPPIEEIMSNSPE"
     gene            complement(2065..>2640)
                     /locus_tag="VGB68_12520"
     CDS             complement(2065..>2640)
                     /locus_tag="VGB68_12520"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0050109.1"
                     /translation="NMINDETLSFLQKGSILLNYSRGDVVDLEALRKAIEKGVVSGAA
                     VDVFPEEPEKNGDSFTTILQNLPNVILTPHIGGSTEEAQANIGLDVTSKLINYMELGT
                     STGSHTVPDLNLPPQAETHRILHIHRNIPGVLGEINSKLSTRGINIVSQYLKTNDEIG
                     YVILDIDTELSKEAFAILKEVKATIKTRMVY"
BASE COUNT          561 a          731 c          637 g          711 t
ORIGIN      
        1 cggtggtcgc cgtttcatct tcataccagt cttcgtgcgg cgagcatccc cggcagcgca
       61 ggcaatcggt ccggctccat tgatgaccgc agcccggaca gcgcccgcgc gtctcgaaag
      121 tgttccaaac cgtgccgcag ccgttatgga aaaaatgcgg ataaccggcg gcgtcgctgc
      181 agcaccagcg gctcgacgcc tgcggctgcc agcgacataa aggacagcga atccggctga
      241 aatccgtcgt ccggtctttt tgcttcgcgg tttccgcgta tttatcaaaa accggacttt
      301 tgcggaaaag aaaaaaggta attgccaggg gctgtaagag attttccaga cgattttccg
      361 acatacttta atttgaagct aaagccgctc cgcaataaat ctggcgacca atccgtttgc
      421 gccgaccgcc caaaccgccc gtttaccttt tgcctgcacg gaattgtaat tttccttatc
      481 gagatttttc cacgttttgc cgccgtcgac ggataaatca gagcccgacg cgccgaccgc
      541 gaccatcgtt tttttatcaa tgtaagccgc gcccgaacga tagcccgtca agccttttcc
      601 cggcttccac gtttttccgc cgtccgttgt aaaagctaaa ttgtttgtcg tttcgtttgg
      661 tttttcgtag tttccgccga caattgcgcc tcgttttgaa tcaaacatcg caatcgaaaa
      721 aattccgctt cccgccgtgc ccctgactat cggagtttcg gagacaaacc agctcaagcc
      781 gcgattattc gagcgaaaaa cgcgcgcgtc atttccgccc gaaacgagaa aaacgtccga
      841 ttttccgctt gtaatcaagc aggttccgct cgcggcaaag gcggcttcgc cttctttggc
      901 gttcgccatt ttctcggctc cgagcggctt ccacgtttcg ccgtcgctcg tcgtcattaa
      961 taaatattta ccgtcgaccg ggtcgctcat cgctatcccg ttgttttcgt cccagaaagc
     1021 gatggcgtcg aaaaaagctt tcgggttcgc gtttttaaac tgcattttcc acgtcgcgcc
     1081 gccgtcggtc gttttataaa tgcgcgaatt ttcgccctcg ccgatgctca aaagataagc
     1141 cgtgttcgca tcgaaggctt cgacgtcgcg aaaatccagt ttttccgcgc cgggaacttt
     1201 gccgaccgtc cagttttttc cgccgtccgt cgttttcaga aagctgccgc ccgtgccgct
     1261 cgcccagatt attttttcgc tgacgacggc aagcccgcgc aggctcgccc tcgtctcaat
     1321 cgtctgtttt tgccattgcg cggcggcgct cgaataaaac ataagcgcga ataacgaaag
     1381 taaaatcaga tgttttttca gatttgtttc gagccgagat tttgtgctga ttttcatatc
     1441 tttagtgtaa actggcggaa atttattcgg gagagttcga cataatttcc tctatcggcg
     1501 gcagtagtcc gagctgctga aaaaatccga gccgttcgac taaaacccaa tccgccgcga
     1561 ttttttcatc tgcaatccgg tgagccgccg tcattctgat gccgacccgt tttccggtcg
     1621 ccggtatgcc gtgccaattt ccggtatgcg tgccgtgcat cgtcgcgtaa gttataacaa
     1681 ggtcgttttc cgcaaccatc cgctcgacgg tgaaatgcaa atcgggaaac gctccgagcc
     1741 attcgccgat gtgttccttt atcgcttcgg cgctgcgcgg cgcggcgacg gtcgccgcgc
     1801 cggattcgag ctggtgcgtc acgcattcgg gcgcgaaaat ttcgtcggct gttgcaagct
     1861 ttcgctcgtt ccaaagctct tcgtaaaagc gccggacaat atttttattt cgctcgatgg
     1921 acatatttta aagctctgcc aggcaaaata ttcaaaaccg atttcatcaa agctaatata
     1981 actcagtccg gcgattataa aaaattttct ctcgcgcctt ttcccgtgtt tcgcgggcgg
     2041 attttcttaa gtcaaatatc ctgtttaata aaccatccgc gtttttatcg tcgctttgac
     2101 ttcctttaag atggcaaagg cttctttgga aagctcggtg tcgatgtcca gaattacata
     2161 gccgatttcg tcgttggtct ttaaatattg actgacgata ttgatgccgc gcgtcgaaag
     2221 cttcgagttt atctcgccca gaacgcccgg aatattccgg tgaatgtgca gaatgcggtg
     2281 cgtttcggct tggggcggca gatttaaatc gggcacggtg tgcgaccccg tagaggtgcc
     2341 gagctccata taatttatca gtttcgacgt cacgtccaga ccgatattcg cctgcgcttc
     2401 ttcggtcgag ccgccgatgt gcggcgtcag gatgacgttc ggcagatttt gcagaatcgt
     2461 cgtaaaagaa tcgccgtttt tttccggttc ttcgggaaaa acgtcaaccg ccgcgccgct
     2521 gacgacgcct ttttcaatcg cttttcgcag ggcttccaaa tcgaccacgt cgccgcgcga
     2581 ataattgagc aaaatactgc ctttctgtaa aaacgaaagc gtttcatcgt taatcatatt
//
LOCUS       DASWNE010000638         2759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_270035, whole genome shotgun sequence.
ACCESSION   DASWNE010000638 DASWNE010000000
VERSION     DASWNE010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270035"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            279..815
                     /locus_tag="VGB68_12525"
     CDS             279..815
                     /locus_tag="VGB68_12525"
                     /inference="COORDINATES: protein motif:HMM:NF044704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEY0050110.1"
                     /translation="MNIRVGQKVAYPNHGVCKVEAIKNTQDGAQTEEFYSLRVLSNNS
                     SILVPKHKAESVGIRPVIKPMECEVLIKFLAEDFENPAGDWKARSREFAAKFQTGDIF
                     AVADVLKKLSFLTLAKPLSFREQRMIEKAKFLVVSELAVVCSQPECAVEEKVDKALAR
                     ACTQHNPSEIKTVCATIH"
     gene            complement(892..1374)
                     /gene="queF"
                     /locus_tag="VGB68_12530"
     CDS             complement(892..1374)
                     /gene="queF"
                     /locus_tag="VGB68_12530"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR03139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEY0050111.1"
                     /translation="MANKKKTEPVNQQGLDIRPTPRGEMNLYALDTFPYEYTGRKIKI
                     NFEMPEFTAICPFSDFPDFAVIRLEYVPNQRCIELKSLKLYINSFRNVKIFHEHVVNV
                     IMEDFVAACDPLKVKIEGDFHVRGNVKTIVRAKYKRRKDKKRDKKNRDSIKLIEVAAE
                     "
     gene            complement(1685..>2759)
                     /locus_tag="VGB68_12535"
     CDS             complement(1685..>2759)
                     /locus_tag="VGB68_12535"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0050112.1"
                     /translation="AIGAIEGLAESTASLLKLFSGYLSDKFGRRKLPVFLGYALSSIV
                     RPLLAFVTTWYHVLFVRLADRVGKGVRGAPRDALLAAEVPPERRGFAFGFNRAADHLG
                     AVLGPVVAFVLLSYFALNPEVPTVQEYQQVFLVASIPVVLGLFVIVFFVKEEGKKELH
                     ETVEKTKIKFSLAAFDSNFKRFLVTIALFTLSNSSDAFLLLRATEAGIAPAVLPLLWM
                     VLHLSKVFFSLVGGDLSDRIGRKTLIFSGWILYALVYAGFAFVTEAWQTWVLFIIYGV
                     YFGLTEGAEKALVADMVTDEKRGTAYGLYALAFGITVFPASLLIGGIWSFYGAPTAFL
                     FSAFLSICAAVLLLTVKTKAVQD"
BASE COUNT          762 a          703 c          655 g          639 t
ORIGIN      
        1 tttgtaattg tttttttatt tttgaaaaac cggtcttcct ctcgtcggaa agcaaagccg
       61 gataatgcgc cgtttgccga taatcaaata ctcatcggac agttaaagaa cttgtctttc
      121 atcgcccgac ggcgtttcga cgccgattgt tttgaacttt ttcaaaaaaa cctttctaat
      181 ccgtcagcct tttgctaaaa ttaagccgca cctcaaaatt tatttttttc agacagcttg
      241 cggtttataa gcttgcctta atcttttgga ggctacgtat gaacattaga gtcggtcaga
      301 aagtcgcgta tccgaatcac ggagtttgca aggtcgaagc aattaaaaac acacaggacg
      361 gcgcgcaaac cgaagagttc tactcgctcc gcgttttatc caacaactct tcgattttag
      421 ttccgaaaca caaagccgag agcgtgggca tccgtccggt catcaaaccg atggaatgtg
      481 aggttttgat aaagtttctg gcggaggatt tcgaaaatcc ggctggcgac tggaaagccc
      541 gctcgcgcga atttgccgcc aaatttcaaa ccggcgatat ctttgccgtc gccgacgttt
      601 tgaaaaaact gtcttttttg acgctggcaa aaccgctttc gttccgcgag cagagaatga
      661 ttgaaaaagc gaaatttctg gtcgtttccg agctcgccgt cgtctgctcg cagccggaat
      721 gcgcggtcga agaaaaagtt gacaaagcgc tcgcccgcgc ctgcacgcag cataatcctt
      781 ccgaaatcaa aaccgtctgc gcgacgattc actaagtcgg aagaccgcgg aaaacgggaa
      841 atggaaaacg aaaaaccgta tcttatcgcg ttttcagttt tccattttcc gttattctgc
      901 ggctacttca atcaatttga tggaatcccg gtttttttta tcgcgttttt tgtctttccg
      961 gcgtttgtat ttggcgcgca caatcgtttt gacgttgccg cgcacgtgga aatctccttc
     1021 gattttgact ttcaaagggt cgcaagccgc gacgaaatct tccataatca cgttgacgac
     1081 gtgttcgtga aaaattttca cattgcggaa agaattgata tagagcttta agcttttcag
     1141 ctcgatacag cgctgattcg ggacgtattc gaggcgaata acggcaaaat ccggaaaatc
     1201 ggaaaacggg caaatcgccg taaattccgg catttcaaaa ttgattttga ttttgcgccc
     1261 cgtatattcg tagggaaaag tgtcgagcgc gtaaagattc atttcgccgc gcggggtcgg
     1321 gcgaatgtcc aaaccctgct ggttgacggg ttctgttttt ttcttgtttg ccatagttat
     1381 ctttttcgtt aaaagtcgaa acgataattt tacaacagcc ggagaaattc gccaaagccg
     1441 cctggcggac gaaaaagatg tcgcgcaaaa ctgaaaactg atgcgcgcaa gcgcgaaatc
     1501 cggtttgcgg cgatgaaaat tcattcgcgc gtttaaaaac cgctttgcgc acgagaaaat
     1561 tcgtcggcgc ggcggttaat acgattttgc gcacgagaaa tttgattcgc gcgtttaaaa
     1621 atcgtcataa gcaaaccaaa ataaatgtct tttgagcttc aagaccggtg gaaattgata
     1681 aaaatcaatc ctgcacggct tttgttttga ccgtcaaaag caggacagcc gcgcagattg
     1741 ataaaaacgc gctaaaaaga aacgccgtcg gcgcgccgta aaagctccag attccgccga
     1801 tgagcaagga tgccggaaaa accgtgatgc cgaaagccag cgcgtagagc ccgtaagccg
     1861 tgccgcgttt ttcgtccgtc accatatcgg cgaccagcgc cttttccgcg ccttccgtca
     1921 agccgaaata aacgccgtaa atgataaaca aaacccacgt ctgccacgcc tcggtgacaa
     1981 aagcgaatcc ggcgtaaacc agcgcataca aaatccagcc cgaaaaaatc agggtttttc
     2041 tgccgattct atccgataaa tcgccgccga ccagcgaaaa gaaaactttg ctcaaatgca
     2101 gaaccatcca caaaagcggc aaaacggcgg gcgcaattcc cgcctcggtc gcgcgcagca
     2161 gcagaaacgc gtccgacgaa ttcgaaagcg tgaaaagcgc gatggtgacg agaaatcgct
     2221 tgaaattgga atcaaaagcc gcgagcgaaa attttatctt tgtcttttcg accgtttcgt
     2281 gcagctcttt tttcccttct tccttgacga aaaaaacgat gacgaacaat cccagaacga
     2341 ccggaatcga ggcgaccaga aaaacctgct ggtattcctg aacggtcgga acctccggat
     2401 taagggcaaa atacgaaagc aaaacgaaag cgacaaccgg accgagaacc gcgcccagat
     2461 gatcagccgc gcggttgaat ccgaaagcga aaccgcgtct ttccggcgga acttccgccg
     2521 ccagcagcgc atcgcgcggc gcgccccgaa cgcccttgcc gaccctgtcc gccagccgaa
     2581 caaacaaaac gtgataccag gtcgtgacaa acgccagcag cgggcgaacg atgctcgaca
     2641 acgcgtaacc cagaaaaacc ggcagttttc gccgcccgaa cttgtcgctc agataaccgg
     2701 aaaaaagctt taaaagcgag gcggtcgatt ccgccaagcc ttcgatagcg ccgatggca
//
LOCUS       DASWNE010000639         2394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_160713, whole genome shotgun sequence.
ACCESSION   DASWNE010000639 DASWNE010000000
VERSION     DASWNE010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160713"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2157)
                     /locus_tag="VGB68_12540"
     CDS             complement(<1..2157)
                     /locus_tag="VGB68_12540"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT and VWA domain-containing protein"
                     /protein_id="HEY0050113.1"
                     /translation="MNKGNAKILFGLILFTFCFAVEAQAQGVIVPIICERRPCVPPPR
                     PVPPRPVIPTALPVKSINIDTRINGQVATTHLEQVFRNDTPYTLEGTYFFPIPETASI
                     VEFAIWENGRKLVGEVRTREEARRIYEEIVRRQRDPGLLEYVGKNLFQASIFPIPPHS
                     DKKLELTYTQVLKAESGTVAYRYPLGTGQRNWLEPNRGETTRGTRPDKVGTVSGRIEI
                     QSKEALRNIYSPSHSIDVKKKGENSAVLTFETSEKNLLDFQLFYGLSAQDFGMTLLTY
                     REAAKDGFFLLLLSPKDNILERELVSKDIVFVLDTSGSMADEGKMEKARSALLFGIRS
                     LRDGDRFNVINFAGEEHLMESGLIAANADGKRRGEEFVKKLQPTGGTNINDALIAAKK
                     QFDRNDRPKILVFMTDGLPTVGETSVEKIIGNFDKEKIDGLRLFNFGVGYDVNTTLLD
                     KLAAENSGAADYVEPKEDLEIKVSNFFGKVNFPVLTDLEIDFGNVQTELLYPRKTPDL
                     FKGSQIAIIGRYKTFDDKAVITLRGRDGRETRTFRYADLDFPQRSEENAFLPKLWAIR
                     RVGWLTEQIRTNGEQKELKDEIVRLGTRYGIVTPYTSYLALENNLQTTAGNEDRNRVF
                     QNSAPKAAKESSGQGAVTLSQNTRSQQSVLTITPSDKADNDIISFSNNYSQQVGNKNF
                     YNVNNVWQDAEFDEKSRNNEVKIQFASDEYFNLLARE"
BASE COUNT          533 a          625 c          599 g          637 t
ORIGIN      
        1 ttctctggca agtaaattga aatattcgtc gctggcaaat tggattttga cttcgttatt
       61 tctcgatttc tcgtcaaatt cggcgtcctg ccagacgtta ttcacattgt agaaattttt
      121 gtttccgacc tgctgcgaat aattattcga gaaagaaata atgtcgttat ccgctttgtc
      181 gctcggcgta atggtcagga cgctttgctg cgacctggtg ttctggctca aagtcaccgc
      241 gccctgcccg ctcgattctt tcgccgcctt cggggcggaa ttctgaaaga cccgattgcg
      301 gtcttcgttt ccggcggtcg tctgaagatt gttttccagc gccagatacg acgtgtaggg
      361 cgtgacgatg ccgtaacgcg tgccgagccg gacgatctcg tctttcagct ctttctgctc
      421 gccgttggtg cgaatctgct cggtcagcca gccgacgcgg cgaatcgccc agagcttcgg
      481 caaaaacgcg ttttcctcgc tgcgctgcgg aaaatccaaa tccgcataac gaaaagttct
      541 cgtttccctg ccgtctctgc cgcgcagcgt tatgacggct ttatcgtcga aagttttata
      601 acgcccgata atcgcgattt gcgagccttt gaataaatcc ggcgttttgc gcgggtaaag
      661 cagctcggtt tgcacattgc cgaaatcgat ttccaaatcg gtcaaaaccg gaaaattcac
      721 ctttccgaaa aagtttgaaa ccttgatttc caaatcttcc ttcggctcga cgtaatcagc
      781 cgcgccggaa ttttcggcgg ctaatttatc gagcagagtc gtgttgacgt cgtagccgac
      841 accgaaattg aaaagccgca agccgtcgat tttttctttg tcgaaattcc cgatgatttt
      901 ttcgacgctc gtctcgccga ccgtcggcaa gccgtcggtc ataaacacga gaattttcgg
      961 gcggtcattg cggtcaaatt gttttttcgc ggcaatcagg gcgtcgttga tattcgtgcc
     1021 gccggtcggc tgaagttttt tgacgaattc ctcaccgcgt cgttttccgt cggcattggc
     1081 ggcaatcaag ccggattcca tcaaatgttc ttcgcccgcg aaatttatca cgttgaagcg
     1141 gtcgccgtcg cgtaaactcc ggatgccgaa cagcagcgcc gagcgcgctt tttccatttt
     1201 gccttcgtcc gccatcgagc ccgacgtgtc gagaacgaaa acgatgtctt tggaaaccag
     1261 ttcgcgctcc agaatattgt ctttcggcga cagaagaagc agaaaaaatc cgtctttcgc
     1321 cgcttcgcga taagtaagca gcgtcatccc gaaatcctgc gccgaaagcc cgtaaaaaag
     1381 ctggaaatcc agaagatttt tttcggaagt ttcaaacgtc agaacggcgc tgttttcgcc
     1441 ttttttcttc acgtcaatcg aatgcgaagg cgaataaata tttcttaagg cttctttgct
     1501 ctgaatctct atgcgtccgg aaaccgtgcc gaccttatcc gggcgcgttc cgcgcgtggt
     1561 ttcgccgcgg ttcggttcga gccagtttct ctgtcccgtg ccgagcgggt aacgataagc
     1621 gaccgttccc gattcggctt ttaaaacctg cgtgtaagtc aattccagct ttttatccga
     1681 gtgcggcgga atcgggaata tcgaagcctg aaataaattt ttgccgacgt attccagcag
     1741 acccgggtcg cgctggcggc gcacgatttc ctcgtaaatg cggcgcgctt cctcgcgcgt
     1801 gcgaacttcg ccgaccagct ttctgccgtt ttcccagatg gcaaattcga caatcgaagc
     1861 cgtttccgga atcgggaaaa aataagttcc ttccagagtg tatggcgtgt cgttgcgaaa
     1921 aacctgttcg agatgcgtcg tcgcgacctg tccgttaatg cgcgtgtcga tattgattga
     1981 tttgacgggc agcgcggtcg gaattaccgg gcgcggcgga accgggcgcg gcggcggaac
     2041 gcacgggcga cgctcgcaga ttatcggaac aatcacgccc tgcgcctgcg cctcgacggc
     2101 aaaacaaaac gtaaacagaa tcagaccaaa taaaattttg gcgtttcctt tattcatact
     2161 ttctccttca gaaactaaaa tgcgcggaac ttcaatggct tcgcttaatt tgacgaatta
     2221 aggaacgaac gaagttgctc cggcttgcat aaaaagtggc aaacggtaga cggcaaacgg
     2281 taagttcgtt gttttgagta tcgagttttt atttaaaatc cgaaattcag cagctgcaac
     2341 ttaccgttta ccactttata agtccacgtt ccggcgtaga cggttttata agct
//
LOCUS       DASWNE010000640         6811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_657961, whole genome shotgun sequence.
ACCESSION   DASWNE010000640 DASWNE010000000
VERSION     DASWNE010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6811
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657961"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..351)
                     /locus_tag="VGB68_12545"
     CDS             complement(121..351)
                     /locus_tag="VGB68_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050114.1"
                     /translation="MPENKPNREQLERRRDEINAQLSRVNEDLQMELDRDPEEQAIQI
                     EHDQVSISMENNLRTELAIIEDQLLDFQDGSE"
     gene            509..1621
                     /locus_tag="VGB68_12550"
     CDS             509..1621
                     /locus_tag="VGB68_12550"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY0050115.1"
                     /translation="MLMNTEPKLNRPVVKWFVIWAVWTLVGFFFASQSVLQNQLSENP
                     MPFWRILSWQLFSGYVWLVLCPVILWLGRRFPFENGRWKTSLPVHLFAGVVVALFQLA
                     VDAFVLPELGYLRRYLSGSFLENYRVFVLVNLHFSVSIYWGVLGIYQAIRYYRKYRER
                     ELQTSQLEARLAMSRLQVLKMQLHPHFLFNTLNAISELIYKDPESAERMIGDLSDLLR
                     MSFQNLEVQEITLKQELEFLRKYLEIEQMRFHDRLRVEMKIAPETLDAQVPNMILQPL
                     VENAIKHGLAPRSEGGKIEIGAERSNGHLQITVSDDGIGVPFNDVENLPEGVGLSNTR
                     KRLRHLYGERHKFALATAEKSGLKVNLTIPFKESEN"
     gene            1734..2501
                     /locus_tag="VGB68_12555"
     CDS             1734..2501
                     /locus_tag="VGB68_12555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY0050116.1"
                     /translation="MKIKTLIVDDEPLARERVKRLLRDETDITVIGECGNGAEAITAI
                     KREKPDLVFLDIQMPEKNGFEVIKSLDAKSVPTIIFVTAYDQYALQAFEVHALDYLLK
                     PFNRDRLHRALARAREHIEHRNFGNLDERLASLIADLKAEKKYLERLVVKSVGRVFFL
                     KTDEIDWIEAAGNYLKLHVGRDSHLIRETMNGIEAKLNPDKFLRIHRSTVVNIDRIKE
                     LHPMFSGDYSVILRNGVELTLSRNYRERFLELFENQT"
     gene            2709..3719
                     /locus_tag="VGB68_12560"
     CDS             2709..3719
                     /locus_tag="VGB68_12560"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEY0050117.1"
                     /translation="MTRKFKFKVLFLLMFSIVIVWRNYVIVIGQTTKAFLVTLNKAEK
                     GSLTIIDPKDMKVVGSVPTGDYPHEVVLSADGKTAFVANYGAQTPGSSLSVIDLEAKK
                     EIRRVDLTPLLRPHGLVETGGKIYFTSETSRTIGRYDPATNRVDWLMGTGQNGSHMIV
                     VTPDQKRMYTANIGSDSVTAFEFQNVPPAASRITHIAVGKQPEAIDISPDGKEVWVGL
                     NAEGAIDIVDATTNKFKEKIKLGERPYRVKFTPDGRYVFATMPNTKEIIVLDAATRKE
                     TRRMKLESVPLGIVFSRDARLAFVSAVENDFVLKIDLEKYEVLGKAASGQSPDGVALG
                     GM"
     gene            3897..5561
                     /locus_tag="VGB68_12565"
     CDS             3897..5561
                     /locus_tag="VGB68_12565"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0050118.1"
                     /translation="MRKFLAFILVLFVLAGAPLESAIAQTRKTGKTNSTNTVAAPRFG
                     NTDGITAQQLKNYLEFIASDELEGRDTPSRGLDIAAMYIADHLKSWGIKPAGDGGSYF
                     QKFPLRRVKVDAQNTRLQLNNQSFVYGEDFLANFTPGSISGSEIVYASHGWVLKSKNI
                     NPYQGIDVRDKIIVVTNSLPKGVTFNDLQGKAGDDWFSPPLYAQANGAKAVIAFGTFG
                     NLANWQGSRWTQTEKGSVEFGKPQTQITIPTLTAGPRLIAALFQGEKSTAGNTYNKAV
                     AGEPVDAFALNPNKKINLSVAVKTENIHSQNVVGILEGSDPVLKNEYVAIGAHYDHVG
                     MNPFSANEDKIWNGADDDGSGTVAVMAMAEAFSKGAQRPKRSILFIWHAGEEKGLWGS
                     EYYAENPTVPINSIVTQLNIDMIGRAQKLNEEVKPRNKELTKQGDVYLIGSKMMSTEL
                     GEISEATNNAFLKMNFNYRYDDPKDPQQFFYRSDHYNYAKKGIPIIFYMDGDHEDYHQ
                     PSDSIEKIDFEQMEKISRTIFATGWELANRPTRPRVDKPLAAQAGN"
     gene            5802..6362
                     /locus_tag="VGB68_12570"
     CDS             5802..6362
                     /locus_tag="VGB68_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050119.1"
                     /translation="MNNLNKLFLLAAIVLAIGAAPVAAQNTPAKKAVEIPVVEPKAED
                     VGSIDGIVKAFYEVISGGVGVPRQWSRDKTLYIADVRFVGMSERGGKIVADVMNHRQY
                     VEGSNDFFVREGFVEKEIKRVVRKFGNMAQVFSSYEFSTSGKQKISGRGLNSIEMFFD
                     GKRWWISAVTWEEERASNPLPKEFLP"
     gene            6423..>6811
                     /locus_tag="VGB68_12575"
     CDS             6423..>6811
                     /locus_tag="VGB68_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050120.1"
                     /translation="MKKLALIFSIISIFSASAFAQKADDSKDALAVVNKLFAEMAAHN
                     PAGIVALHTPEAQLAAAIKNKEGKSVIRTFAAEAFSKNFAEKKSELKEEMYAPETKIF
                     GDLALVWGRYVFFIDGKISHCGVNAFHL"
BASE COUNT         1755 a         1723 c         1763 g         1570 t
ORIGIN      
        1 atccgatgtg accgctttcg gaagcagtca caaatagtta gttcgggctg ccgtttaatc
       61 agataacaag aaaatcagcc cgcgcgggct gatttcttac ttttcttaaa ttcttccgag
      121 ttattcgctc ccgtcttgaa aatcgagcag ctggtcttcg attatcgcca gctctgtgcg
      181 cagattgttt tccatcgaaa tcgatacctg gtcgtgttcg atttgaatcg cctgttcttc
      241 cgggtcgcgg tcgagctcca tctgcaaatc ctcgttgacg cggctcagct gggcgttgat
      301 ttcgtcgcgg cgacgctcta attgttctct gttcggtttg ttttccggca taaattctcc
      361 cttagataat tttaatttta aaagtataac gcctgcggcg cgcggatgtg aaggggcgcg
      421 agcgggcgct cgaaaatcgc agttggtcag aaaatttcgt ctgttcgtca catccggctt
      481 gccgaaaaag cgtttttagg gctgaataat gcttatgaac actgagccga aattaaaccg
      541 ccccgtagtc aaatggtttg tcatctgggc ggtttggacg ctggtcggat tttttttcgc
      601 cagccagtca gtcctccaga atcaactctc ggaaaatcct atgccgttct ggcgcattct
      661 ctcgtggcag cttttttcgg gctacgtctg gctggtgctg tgtcctgtga ttttatggct
      721 cgggcggcgg tttccgtttg aaaacggcag gtggaaaaca agtctgccgg ttcacctgtt
      781 tgccggcgtc gtcgtcgcgc ttttccagct tgccgtcgat gcttttgttt tgccggaact
      841 cggttattta agacgctatc tgtcggggtc ttttttagag aattaccgcg ttttcgtgct
      901 ggtcaatctg catttcagcg tatcgattta ctggggcgtg ctcggcattt accaggcaat
      961 caggtattac cggaaatacc gcgagcgcga attgcagacc tcgcagctcg aagcccggct
     1021 ggcgatgtcg cgcctgcaag tgctgaaaat gcagcttcac ccgcattttc tgttcaacac
     1081 gctgaacgcg atttccgaat tgatttataa agacccggaa tcagccgagc ggatgatcgg
     1141 cgatttgagc gaccttctgc gaatgtcttt tcagaatctg gaagtgcagg aaatcacttt
     1201 aaaacaggaa ctggaatttc tgcgcaagta tctggaaatc gaacagatgc gttttcacga
     1261 ccgtttaaga gtggaaatga aaatcgcgcc cgaaacgctc gacgcgcagg tgccgaatat
     1321 gattctccag ccgctggtcg aaaacgccat caaacacggg ctcgcgccgc gctccgaggg
     1381 cggaaaaatc gaaatcggcg ccgagcgcag caacgggcat ttgcagatta ccgtcagcga
     1441 cgacggcatc ggcgtgccgt ttaacgatgt ggagaatctg ccggagggcg tcgggctttc
     1501 caacacgcgc aaacgcctgc gacatcttta cggcgagcgc cacaaattcg ctttggcgac
     1561 ggcggaaaaa agcggcttga aggtgaatct gacgattcct tttaaagaat cggaaaacta
     1621 atgaaaagtg aaagtgcgaa aaagtgaaaa ggtgaaaaag cttgccgccg gaaaatgacc
     1681 gtcgcttttt cacttttacc gtttttcacc ttttcacttt taccctttgt cctatgaaaa
     1741 tcaaaacctt aatcgtcgac gacgagccgc tggcgcgcga gcgcgtcaag cgcctgctgc
     1801 gcgacgaaac cgacatcacg gttatcggcg agtgcggcaa cggcgcggaa gccatcacgg
     1861 caatcaagcg cgaaaagccc gacctcgttt ttctcgacat ccagatgccg gaaaaaaacg
     1921 gttttgaggt tatcaaatcg ctcgacgcaa aaagcgtccc gacgataatt tttgttaccg
     1981 cctacgacca atacgcgctt caggcattcg aggttcacgc gctcgattat ctgctcaagc
     2041 cttttaaccg cgaccggctt caccgcgcgc tcgcccgcgc tcgcgaacat atcgaacacc
     2101 ggaatttcgg caatctcgac gagcgcctgg catcgcttat cgccgatttg aaagcggaaa
     2161 aaaaatatct ggaacgcctg gtcgtcaaat ccgtcgggcg cgttttcttt ctgaaaaccg
     2221 acgaaatcga ttggattgaa gcggcgggaa attacctgaa gctgcacgtc gggcgcgatt
     2281 cgcacttgat acgcgagacg atgaacggca tcgaagccaa attaaatccc gataaattcc
     2341 tgcgcatcca ccgctcgacc gtcgtcaaca tcgaccgcat caaagagctt cacccgatgt
     2401 tcagcggcga ttattccgtg attttgcgaa acggcgtcga gctgacctta agccgcaact
     2461 accgcgagcg ctttctcgaa cttttcgaaa accaaacctg ataactgata gttgatagtt
     2521 gataactgat aactgaaagt tgttttgctc taactatcag ttatcagtta tcagctatca
     2581 gttattaata ttgatttcag aaccgttcat cacactcctt cttcacttcg tcacataatt
     2641 gttttccgcc gtaaataaaa ggttaaaatt ccgccagcaa ttaattcgtt ccggcaactg
     2701 gagtccttat gacacgtaaa tttaaattca aggttttgtt tttattgatg ttttccatcg
     2761 tcatcgtctg gcgaaactac gttatcgtta tcgggcagac gacgaaagcc tttctcgtca
     2821 cgctcaataa agccgaaaag ggaagcctga ccatcatcga cccgaaagat atgaaggtcg
     2881 tcggctcggt cccgaccggc gattacccgc acgaagtcgt tttatcggct gacggcaaga
     2941 ccgcgttcgt cgccaattac ggcgcgcaga cgcccggcag ctcgctgtcg gtgattgatt
     3001 tggaagcgaa aaaagagatt cgccgcgtcg atttgacgcc gctgcttcgc ccgcacggac
     3061 tcgtcgaaac gggcggaaaa atttatttca cgtcggagac cagccggact atcgggcgtt
     3121 acgacccagc aacaaatcgt gtggattggc tgatgggaac cggacaaaac ggctcgcaca
     3181 tgattgtcgt cacgcccgac cagaaaagaa tgtatacggc gaatatcggc tcggattcgg
     3241 tgacggcttt cgagtttcaa aacgtgccgc ccgccgcttc gcgcattacg cacatcgcgg
     3301 tcggcaaaca gcccgaagcg attgacatct cgcccgacgg caaagaggtc tgggtcggtc
     3361 tgaacgccga gggcgccatc gacatcgtcg atgcgacgac caataaattc aaggaaaaaa
     3421 tcaaattggg cgagcgcccg tatcgcgtca aatttacgcc cgacggacga tacgtgttcg
     3481 ccacgatgcc gaacacgaaa gagattatcg tgctcgacgc ggcgacgcga aaagagacgc
     3541 ggcggatgaa gctggaaagc gtcccgctcg gtatcgtctt ttccagagac gcccggctgg
     3601 ctttcgtttc cgccgtcgag aatgattttg ttttaaaaat cgatttggaa aaatacgaag
     3661 tcctcggcaa agccgcgagc gggcagtcgc ccgacggcgt agcgctgggc ggaatgtaaa
     3721 aaaaaataaa tgatttcgga ttataaaaaa ttgcccgcgg aaagattcgg aaaacgcctt
     3781 aactgtttat ttttaactga tttataccaa attaatttat aaacatcatt catccggcgt
     3841 ctttcgaagg gctttcgcgg gcaatcgctc cggcaaaata tctagtgagg aaaataatga
     3901 gaaaattttt agcctttatt ttagttttat ttgttctggc gggcgcgccg ctcgaatcgg
     3961 caatcgctca aaccagaaaa accggcaaga caaattcgac caacacggtc gccgcgccgc
     4021 gattcggcaa tacggacggc atcacggcgc agcagttaaa aaattatctg gaatttatcg
     4081 cctcggacga actcgaagga cgcgacacgc cctcgcgcgg gctggacatc gcggcgatgt
     4141 atatcgccga ccatttaaag agctggggaa tcaagccggc gggcgacggc ggcagctatt
     4201 ttcagaaatt tccgctgcgc cgcgtcaagg tcgatgcgca aaacacccgc ctgcaattaa
     4261 ataatcaatc tttcgtttac ggcgaagatt ttctggcaaa ctttacgccg ggctccatct
     4321 ccggcagcga aatcgtctac gccagtcacg gctgggtttt gaaatcgaaa aacatcaacc
     4381 cgtatcaggg catcgacgtg cgcgacaaaa tcatcgtcgt caccaacagt ctgccgaaag
     4441 gcgtgacgtt taacgatttg cagggcaaag cgggcgacga ctggttttcg ccgccgctct
     4501 acgcgcaggc gaacggcgcg aaagccgtca tcgccttcgg cactttcggc aatctggcga
     4561 actggcaggg ctcgcgctgg acgcagaccg aaaaaggcag cgtcgaattc ggaaagccgc
     4621 agacgcaaat cacgattccg accctcaccg ccggtccgcg tcttatcgcc gcgctgtttc
     4681 agggcgaaaa atcaaccgcc ggaaatactt ataacaaagc cgtagcgggc gagccggtcg
     4741 acgcgtttgc tttgaatccg aataagaaaa tcaatttgag cgtcgcggtc aaaaccgaaa
     4801 atattcactc gcaaaacgtc gtcggcattc tcgaaggctc cgacccggtt ctgaaaaacg
     4861 aatacgtcgc catcggcgct cactacgacc acgtcgggat gaacccgttt tcggcaaacg
     4921 aagataaaat ctggaacggc gcggacgacg acggttcggg aacggtcgcg gtgatggcga
     4981 tggcggaagc gttttcgaaa ggcgcgcagc gtccgaaacg ctcgattctt ttcatctggc
     5041 acgcgggcga agaaaaaggc ttgtggggca gcgaatatta cgccgagaat ccgaccgtgc
     5101 cgattaattc gattgtcacg cagctcaata tcgatatgat cgggcgcgcg caaaaattga
     5161 acgaagaagt caagccgcgc aataaggagc tgaccaaaca gggcgacgtt tatttaatcg
     5221 gctcgaaaat gatgagcacg gagctcggcg aaatcagcga ggcgacgaat aacgcgtttt
     5281 taaaaatgaa ttttaattat cgctacgacg acccgaaaga cccgcagcag tttttctatc
     5341 gctccgacca ttacaattac gcgaaaaaag gcattccgat aattttctat atggacggcg
     5401 accacgaaga ttatcaccag ccgtcggatt caatcgagaa aatcgatttc gagcagatgg
     5461 agaaaatttc gcggacgatt tttgcgacgg gttgggagct ggcaaatcgc ccgacgcgtc
     5521 cgagagtcga taaaccgctt gcggcgcagg cgggaaatta gccgccaata atgaggagaa
     5581 gcccgcgcgc cgggcaatgg cgcgaacggt ttaagaagat ggaacgccga tttttaggat
     5641 tgcctgagat aatcgggatt tcgattccag tttatcttcc cgaaaaaccg gcttcctgtt
     5701 cttaaaaagg aaaccacgga ttgatacaga taaatacaga taatttactc ctctatctgc
     5761 aataatacgt gttcattccg tggtttcaca tttttcggat tatgaataac ttaaacaaac
     5821 tgtttctgct ggcggcaatc gttttagcaa tcggcgcggc tccggtcgcg gcgcaaaata
     5881 cgcccgcgaa aaaagcggtt gaaattcccg tcgtcgaacc gaaagccgaa gacgtcggct
     5941 cgatcgacgg catcgtcaaa gcgttttacg aagtgatttc gggcggcgtc ggcgtgccgc
     6001 gccaatggtc gcgcgacaaa actttgtata tcgccgacgt tcgctttgtc gggatgagcg
     6061 agcgcggcgg caaaatcgtc gccgacgtga tgaatcaccg ccaatacgtc gaaggctcaa
     6121 acgatttttt tgtcagagaa ggctttgtcg aaaaagaaat caagcgcgtc gtcagaaaat
     6181 tcggcaatat ggcgcaggtt ttcagcagct acgaattttc gacttccggc aaacagaaaa
     6241 taagcgggcg cgggctaaac agcatcgaga tgttttttga cggcaaacgc tggtggattt
     6301 cggcggtgac ctgggaagaa gaacgcgcga gcaacccgct tccgaaagag tttctgccgt
     6361 aaaagaattt gcccgccaaa gacgcgaaag aataaaatat taaataagga cgaattaaaa
     6421 aaatgaaaaa actagcactt atcttttcaa ttatctccat tttctcggcg tcggcttttg
     6481 ctcagaaagc cgacgattcg aaagacgctt tagccgtcgt caacaaattg tttgcggaaa
     6541 tggcggcgca taacccggcg ggaatcgtcg cgcttcatac gcctgaagcg cagctggcgg
     6601 cggcgataaa aaacaaggaa gggaaatccg tcatcaggac ttttgccgcc gaagcgtttt
     6661 ccaaaaattt cgcggaaaag aaaagcgagc tgaaggaaga gatgtatgcg ccggaaacta
     6721 aaatcttcgg cgatttggcg ctcgtctggg gcagatacgt attttttatc gacggcaaaa
     6781 tctcgcattg cggcgtcaac gcgtttcacc t
//
LOCUS       DASWNE010000641         1615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_57538, whole genome shotgun sequence.
ACCESSION   DASWNE010000641 DASWNE010000000
VERSION     DASWNE010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57538"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..342)
                     /locus_tag="VGB68_12580"
     CDS             complement(61..342)
                     /locus_tag="VGB68_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050121.1"
                     /translation="MEKENSINRRDFVKSAGKRRSRKREKLNMPQIEGGHAGGDDRLR
                     DAIFKNAPVPDYLKLPGARAVAMSCLTGIAARASIEKKRPVKISELIRL"
     gene            complement(352..>1615)
                     /locus_tag="VGB68_12585"
     CDS             complement(352..>1615)
                     /locus_tag="VGB68_12585"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEY0050122.1"
                     /translation="GYGAVADGKTLSTEAFRKAIDECSRKGGGRVVVPAGTFLTGAIH
                     LKSNVNLHVSKDAIIKFSTDAKDYLPIVHTRWEGMELMHLSPFIYAYEQTNIGITGEG
                     TLDGQGKAMFWKWHGNPRYGGNPQVLSQRPARARLYEMMEKGVPVAERIFGDGHYLRP
                     QFIQPYKCKNVLIEGVKIIDSPMWEVHPVLCENVTIRRLHISSHGPNNDGCDPESCKD
                     VLIEDCFFDTGDDCIAIKAGRNEDGRRINVPTENVVVRGCTMRDGHGGVTVGSEISGG
                     VRNVFTENCKMDSPDLWTALRVKNNASRGGRLENFYFRNITVGVVSRAVVEIDFNYEE
                     GAKGKYVPVVRNYVVENLTCGKGNRAVDLQGLDNAPIYDIVMKNCTFEAIEKPSVVKN
                     VRGVKLENVKINGKIVDRLENAASKKTA"
BASE COUNT          326 a          474 c          401 g          414 t
ORIGIN      
        1 cgccgaagcc gaaacgctga cgacgaaaat taaagataaa aggctcagca gcttcataat
       61 ttacaaccga atcaactccg aaatcttcac cggacgcttt ttctcgatgc tcgcccgcgc
      121 cgcgattccg gtcagacacg acattgcgac ggcgcgcgcg cccggcaatt ttaaataatc
      181 gggaaccggc gcgtttttga aaatagcgtc gcgcaggcgg tcgtcgccgc ccgcgtgtcc
      241 gccctcgatt tgcggcatat tcagcttctc gcgcttgcgg ctgcggcgct tgccagccga
      301 tttgacgaaa tctcgacgat tgattgaatt ttccttttcc ataaaatttt ctcaggcggt
      361 tttcttcgac gccgcgtttt ccagacgatc gacgattttc ccgttaattt ttacgttttc
      421 cagctttacg ccccggacgt ttttcacgac gctcggtttt tcaatcgctt cgaacgtgca
      481 gtttttcatc acgatgtcgt aaatcggcgc gttatccaat ccctgcaaat cgaccgcgcg
      541 attgcctttt ccgcacgtca gattttcgac cacgtaatta cgaacgaccg gaacgtattt
      601 gcctttcgcg ccttcctcgt agttgaaatc aatctcgacg acggcgcgtg aaaccacgcc
      661 gaccgtaatg tttcggaaat aaaaattctc cagtcgcccg ccgcgcgaag cgttgttttt
      721 gacgcgcaga gccgtccaca aatccgggct gtccatcttg cagttttccg tgaaaacgtt
      781 tcgcacgccg ccggaaattt cgctgccgac ggtcacgccg ccgtgaccgt cgcgcattgt
      841 gcagccgcgc acgacgacgt tttcggtcgg cacgtttatt cggcgaccgt cctcgtttct
      901 gcccgctttg atggcgatgc agtcgtcgcc cgtgtcgaaa aagcagtctt caatcaaaac
      961 gtctttgcac gattcggggt cgcagccgtc attgttcgga ccgtgcgacg aaatatgcag
     1021 ccggcgaatt gtcacgtttt cgcacaacac gggatgaact tcccacatcg gcgaatcgat
     1081 aattttgacg ccctcgatca aaacgttttt gcatttgtag ggctgtataa attgcggtct
     1141 gagataatga ccgtcgccga aaatcctttc ggcgacggga acgccttttt ccatcatttc
     1201 ataaagccgg gcgcgcgccg gtctctggct caagacttgc ggatttccgc cgtagcgcgg
     1261 atttccgtgc catttccaga acatcgcttt cccttgcccg tcgagcgttc cttcgcccgt
     1321 gatgccgata ttcgtctgct cgtaggcgta gatgaacggc gacagatgca tcaattccat
     1381 gccttcccag cgcgtgtgga caatcggcag ataatctttc gcgtcggtcg agaatttaat
     1441 aatcgcgtct tttgagacgt gcagattgac gttcgatttc agatgaatcg cgccggtcaa
     1501 aaacgttccc gccggaacga cgacgcgccc gccgcctttt cggctgcatt cgtcgattgc
     1561 cttgcggaaa gcttccgtcg aaagagtttt gccgtccgcg acggcgccgt atccg
//
LOCUS       DASWNE010000642         3532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_691844, whole genome shotgun sequence.
ACCESSION   DASWNE010000642 DASWNE010000000
VERSION     DASWNE010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691844"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VGB68_12590"
     CDS             complement(<1..383)
                     /locus_tag="VGB68_12590"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY0050123.1"
                     /translation="MRILSLTKQTRRGAENKFFRVLILIGLCAFFSLANSSCAQTKNP
                     ARESPAETKPRAANPPRVTQVDDGKIKQLLKPVGKPLLVNFWATWCGPCREEFPDLVK
                     IDAEYRGKIDFFTVSLDFIEELNTGV"
     gene            complement(393..995)
                     /locus_tag="VGB68_12595"
     CDS             complement(393..995)
                     /locus_tag="VGB68_12595"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEY0050124.1"
                     /translation="MRRKSIFPVAILALLAFVATACSEPEFVPKGDFAVGARLENFTL
                     PDLSGAEQSFDKLKGENGTVLVWVSAQCPVVKSYNERINQIADELKAKGINLIGINSN
                     ATESLDYAASGAKEAGYKFPVLIDKGNVLADKLGASVTPEFYYFDKENVLLYHGALDN
                     DRSGKNVTINYLRDAFDSALSKQLIKTAKTNAIGCSIKRG"
     gene            complement(1030..2364)
                     /locus_tag="VGB68_12600"
     CDS             complement(1030..2364)
                     /locus_tag="VGB68_12600"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY0050125.1"
                     /translation="MRIKILPLILLALAFFTAAHAQTLDKAKLDRFFDRLAEKNKAMG
                     SMTLVKDGKVLYSRAIGYSQINDSQKKPSTAATRYRIGSITKMFTAVMIFQLVEKGKL
                     KLSDTLDKFFPQVPNAGKITVAQMLAHRSGIYDFTNDSDFRSWKMNAKTEDEMLAIIA
                     KGKPVFEPGEKTEYSNSGFLLLGYIIEKVGGKPYGEALKERITSKIGLKDTYLGSGKT
                     DVGKNESFSFNYVGDWKQETETDLSIPGGAGAIVSTPGDLTGFIQALFDGKLVSQANL
                     AAMKPIKDKYGSGIILYPLEGKTFYGHGGGIDGFSSLLIYQPEEKLALACSTNGKGYP
                     LTRIVDGVLDIYQNKPFQIPDFETVAVSPEVLEKYVGVYSSPATPLKLTITRDGSTLQ
                     AQATGQPSFPLEATAQDKFKVESRGIVLEFDAAKNQVTFKRGKQELVFTKEQ"
     gene            complement(2888..>3532)
                     /locus_tag="VGB68_12605"
     CDS             complement(2888..>3532)
                     /locus_tag="VGB68_12605"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY0050126.1"
                     /translation="EKPGRAVDKIIERKGDKIMRKIIACEFMTIDGVIQTEECGGDGF
                     KYGGWFFGYADEVTGAVVQERLRKPVDLLLGRKTFDVWEPYWPTHSDFWPNAMTATKY
                     VASNTRDSSEWQPSVFLSGDIAEKLSELKQQDGPDLHVMGSADLLQTLFKNDLVDELQ
                     LMIFPITLGTGKQLFTDGTIPAAFKVTESQTTPNGIIIVNYERDGEIKTGTIGG"
BASE COUNT          845 a          866 c          818 g         1003 t
ORIGIN      
        1 acacccgtgt ttaattcttc gataaaatcc agcgaaacgg tgaaaaagtc gatttttccg
       61 cgatattcgg cgtcgatttt gacaaggtcg ggaaattctt cgcggcacgg accgcaccac
      121 gtcgcccaga aattaacgag cagaggcttg ccgacgggtt tgagaagctg tttgattttc
      181 ccgtcgtcaa cttgcgtcac gcgcggcgga tttgccgccc gcggtttcgt ttcggcaggc
      241 gattcccggg cgggattttt cgtctgcgcg caggaggagt tggcgaggga gaaaaacgcg
      301 caaagaccta ttaaaatcaa gacgcggaaa aatttattct ccgcgccgcg ccgcgtctgt
      361 ttggttaaac ttaaaattct cataatgcaa gcttatccgc gtttaatcga gcagccgata
      421 gcgtttgttt tcgcagtctt aatcagttgt ttgctcagcg ccgaatcaaa agcgtcgcgc
      481 aggtaattga tcgtcacgtt ttttcccgaa cggtcgttgt cgagcgcgcc gtgataaagc
      541 aaaacgtttt ccttatcgaa atagtagaat tccggcgtga cgctcgcacc gagcttgtcc
      601 gccaaaacgt ttcctttatc gatcaaaacg gggaatttgt aaccggcttc cttagcgcct
      661 gatgcggcat aatccaaaga ttcggtcgca tttgaattga tgccgatcag gttgatgcct
      721 ttggctttta attcatcggc aatctggttg attctttcgt tgtaggattt aacgaccggg
      781 cactgcgccg aaacccaaac aagaaccgta ccgttttcgc cttttaattt gtcaaacgac
      841 tgctcggcgc cgctcaaatc cggcaaagta aaattttcca gcctggcgcc gacggcaaaa
      901 tcacctttcg gaacgaattc cggttcgctg caggcggttg caacaaatgc cagcagagca
      961 agaatcgcaa ccggaaaaat gctttttcgt ctcataaaat tttgctcctt tttattagta
     1021 aaactacggt tattgctcct tcgtaaaaac aagctcctgc ttgccgcgtt taaatgtcac
     1081 ctgatttttc gcggcgtcga attccagaac tattcccctg gattcgactt taaatttatc
     1141 ctgcgccgtc gcttcgagcg ggaaggacgg ctgcccggtc gcttgcgcct gaagagtcga
     1201 accgtcgcgg gtaatcgtta gtttaagggg agtcgccgga ctcgaataaa ctccgacgta
     1261 tttttccaga acctccgggc tgacggcgac ggtttcaaag tcgggaatct gaaacggttt
     1321 attttggtaa atatccaata cgccgtccac aatcctggtt aacgggtaac cctttccatt
     1381 cgtagagcag gcgagtgcga gcttttcttc cggctggtag atcaagagcg agctgaagcc
     1441 gtcaatgccg ccgccgtgac cgtagaaggt tttgccctca agtggataca ggattattcc
     1501 cgaaccatat ttgtccttga taggtttcat cgccgcgaga ttcgcctgcg agacgagttt
     1561 gccgtcaaac agcgcttgaa tgaatccggt caaatcgccc ggcgtcgaaa caatcgcgcc
     1621 cgcgccgccc ggaatgctca aatcggtttc ggtttcctgt ttccaatcgc cgacgtaatt
     1681 gaaggaaaaa ctttcgtttt tgccgacatc ggtttttccg ctgccgaggt atgtatcctt
     1741 gagcccgatt ttcgaagtga ttctttcttt taatgcttct ccgtaaggtt tgccgccgac
     1801 tttttcgatg atgtagccta acagcaaaaa acccgaattg ctgtactcgg ttttttcgcc
     1861 cggctcgaaa acgggcttgc ccttggcgat aatggcgagc atttcatctt ccgttttcgc
     1921 attcatcttc cacgaacgga aatcactgtc gtttgtaaaa tcgtaaatcc cgctgcggtg
     1981 tgcaagcatt tgcgcgacgg taattttccc cgcgttcgga acctgcggga agaatttgtc
     2041 gagcgtgtcg gaaagcttta attttccttt ttcgacaagc tgaaaaatca tcacggcggt
     2101 gaacattttc gtgatcgagc cgatccggta tctggtcgcc gccgttgaag gtttcttctg
     2161 gctgtcgttg atctggctgt agccgatagc gcggctgtaa agaaccttgc cgtctttaac
     2221 cagtgtcata ctgcccatcg ctttattctt ttcggcgagg cggtcaaaaa accggtcgag
     2281 tttggcttta tccagcgtct gcgcgtgagc ggcggtgaaa aatgccagtg ctagtaaaat
     2341 taagggtaat attttgattc tcataatatg tattatgtat aacgtttgtc attcgaattt
     2401 attaacaaga gccaaaggat tttagtttcg ggattttcgg tcatccgtgg gaacggaaat
     2461 tttgcgtttg gggagataaa ctcttagccg aatttgagaa aaataagcct tttctgtttg
     2521 aaaagattat caggcaactt tgaaaattga atgactcaaa aagccccttt tcgagccgat
     2581 taaaagagag tgatatttgt cccataaagg agcctttttg agactgattt tagagtaatg
     2641 ttaattaaaa actataaaac cgcagaaata acccgaaaat agaaagtcta ttttcgggtt
     2701 atggattcag ccacaattag ctaagagtct aaaagttatc cttcaactaa tcatctgaag
     2761 aacaatcgat ttcagcacta ttctcagctg cgcctccgcc ggatgccgat gttctataaa
     2821 ccgtgcggcg aagagattat ctttacttaa tctaaaaatt cttctttgga ataaatttta
     2881 tttatcatta accgccaata gtacctgttt taatctcgcc gtcccgctcg tagttcacaa
     2941 taatgatgcc gtttggggtg gtttggctct cggtcacctt gaaggctgcc gggatggtgc
     3001 cgtctgtgaa caactgcttt ccggtgccga gcgtgatcgg aaatatcata agctgcagct
     3061 catcgacaag atcattttta aagagcgtct gaagcagatc ggcgctgccc attacgtgca
     3121 aatccggtcc atcctgttgt tttaactcgc taagtttttc cgcaatgtct ccgcttaaaa
     3181 agacacttgg ctgccattcg cttgagtcac gcgtattcga ggcgacgtat tttgttgcgg
     3241 tcatcgcatt cggccagaag tccgaatgcg tcggccaata cggttcccaa acgtcaaagg
     3301 tcttccgtcc caacagcaga tctaccggct tcctcagccg ctcctgcacc acggctcccg
     3361 tcacttcgtc cgcatagccg aaaaaccacc cgccatactt gaagccgtcg ccgccgcact
     3421 cttcagtctg tatgacaccg tcaattgtca taaattcaca cgcaattatt tttctcatta
     3481 ttttgtctcc ttttctctcg ataattttgt caacagctct tccaggtttt tc
//
LOCUS       DASWNE010000643         7749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_333621, whole genome shotgun sequence.
ACCESSION   DASWNE010000643 DASWNE010000000
VERSION     DASWNE010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7749
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333621"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VGB68_12610"
     CDS             <1..307
                     /locus_tag="VGB68_12610"
                     /inference="COORDINATES: protein motif:HMM:NF014819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ACP synthase"
                     /protein_id="HEY0050127.1"
                     /translation="RIETKAIKIAFGEYAYKIPMSATKSQLGHPQGASGAAGIGAALL
                     ALNENIIAPTINLDETDEACDLDYVPNASRAAKVETALCNCIGFGSKNSALILGKVK"
     gene            complement(329..1336)
                     /locus_tag="VGB68_12615"
     CDS             complement(329..1336)
                     /locus_tag="VGB68_12615"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HEY0050128.1"
                     /translation="MNVIFLGAGASKALGFLGGNEILPKIIENLSNETLFADYDRAKE
                     KRALLKKTLRQLIPGLSSSARDLSITDVLSLIDFSISSGITLLPKDGFFELRRLRHLV
                     DAAIIEVLNVEPDSNENIRLFADVLRRFKQTVVITSNYDLIPERVFQKAGIPAAELDY
                     GFDWRRVTTGKIIRRPADGNSYALLKLHGSLNWLKCPSCEQIYINLLYAIADLGDFSD
                     SRNYGSEVTCHCGYAPLRSLLITPSLARFHPEAQLKQLVLAGIEYISQASSVTIIGYS
                     MPSEDLAIRSMFIRGMRANKNQPKVKVFQISDAEAERYRAFFPKMDYFTSGLDGFLKS
                     F"
     gene            complement(1409..2431)
                     /locus_tag="VGB68_12620"
     CDS             complement(1409..2431)
                     /locus_tag="VGB68_12620"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEY0050129.1"
                     /translation="MQKSLAAIFSLLFLYTIFAVNAGHQAQQKVAPSQRRLNWYKGNT
                     HTHTINSDGDSTPDEVVRWYRERGYNFLVLSDHNFLTNVEGLNALHGADEKFVVIRGE
                     EVSDTFNKKPIHVNGLNVERAVKPQGGKSVVEVIQRNVDAIRDARGVPHINHPNFGWM
                     ITADDLKQVENNKLFEIYNGHPEVNNFGGGGKPGLEEMWDAILSGGKLLYGIAVDDAH
                     VFKKPWDKSAPRPGQGWIFVRAERLSSAAILEAMERGDFYASTGVELADYQVSDKNIA
                     VTINEERTSKYRVQFIGKGGRVLSEAITNPAVYQFRGDEAYVRAKIIESNGKIAWTQP
                     VMPGKN"
     gene            complement(2541..3857)
                     /locus_tag="VGB68_12625"
     CDS             complement(2541..3857)
                     /locus_tag="VGB68_12625"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY0050130.1"
                     /translation="MKRETMEKSESTEYYFVSDLHFGGDGQLQICDYTEEFIAFLQQL
                     AAEDKDIELIIAGDTFGFWELTGVEGTAQLEEIIKHHTRIFEQLRATGEKIKITLMVG
                     NHDYDLACYPEFVPMLASYNITLDLSMAVVRETGGRKIWVEHGQQIDPYNAATEYGNK
                     NALPLGYFITEALVSGASKYSVFGTSDWLKDIRSVDVRQIPDWLISNYFYNEMNVFLR
                     WLLLPFLLLLTVTIFALLAQMLKYLGIFDVNILLENSFIRWLGLAGDIVRWILTASMM
                     VWFFVFVIAIPLYFVYRDIRRTLFRMQIFPSYKSAPTNEANNIYLDRARTIFKENPAV
                     CAYIFGHTHDAFLVEEPEGRVIINTGTWLKILKRVSVRFGLLPGIYYPTFRLNYFKIR
                     SENEKIIVEYVEIPKEPEEKLTLLQRFLIIGRKPELGKPIAPKTVL"
     gene            3912..5078
                     /locus_tag="VGB68_12630"
     CDS             3912..5078
                     /locus_tag="VGB68_12630"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEY0050131.1"
                     /translation="MNVTNLFCSACDKTYAPNRLYNLCAACGKPLLVAYDLEKAAATL
                     TKEALKSRIPSLWRYEEVLPVENPENRVTLGEGMTPLHRAENLGRKLNLKNLFIKDES
                     ANPTASFKARGMTAAVSMAKELGVKKLAAPSAGNAAGALAAYAAKAGMEAFLFMPRDT
                     PQANIVECEQMGANVTLVDGLITDCGKIVAERKAAEGWFDVSTLKEPYRIEGKKTMGY
                     EIAEQMNFELPDVIFYPTGGGTGLIGMWKAFDEMEKLGWIGDKRPRMISVQAAGCAPI
                     VEGFEQHLNHGAEIENAHTVASGLRVPKAIGDFIILEIVRKSGGSAIAVTDEELISDV
                     REIGAGEGIFPAPEGAACLSALKKMIATGTIESDEHIVLFNTGSGVKYLEAFQA"
     gene            complement(5261..5767)
                     /locus_tag="VGB68_12635"
     CDS             complement(5261..5767)
                     /locus_tag="VGB68_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050132.1"
                     /translation="MATTKASSKTGAKKAAPDKTATKAPAAKAAAKPDGKTPAAKSNA
                     AKNTAAKPAAAKAPTAKKTTSAAKAPAKKAAATAAKPAAKSATIKNAAKPAAKAPAKT
                     AAAKPAAKTAAKPAAKKKADSGIIGKAQKVAGEVVGAATEAVKGAAEAVGVTGGKKAP
                     KKGGKSKK"
     gene            6091..6783
                     /locus_tag="VGB68_12640"
     CDS             6091..6783
                     /locus_tag="VGB68_12640"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0050133.1"
                     /translation="MFQKRSTKLELIDTGDYTAQEYEGCLRELRRVNHYLGDTWALRK
                     TLLREIENKNPPQFSVLDVGAGSGELLRAIAERAKGERSARLVGLELNARSARAILEE
                     SKGFAEIFSIRADALRLPFADASFDYAICSLFTHHFTDEKVVRILSEMKRVARRKIFV
                     IDLHRHALAYFLYTTAGKLFLYNRLIRHDGALSILRSFKPDELKDLAAAAKLENVSVE
                     RHFPFRLVLSGS"
     gene            complement(6891..>7749)
                     /locus_tag="VGB68_12645"
     CDS             complement(6891..>7749)
                     /locus_tag="VGB68_12645"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0050134.1"
                     /translation="IRRVRFHPYIACFPVRTETLPPATHTNCFIAGEREFVVVDAASP
                     FVEEQSALHEYIDSLIENGNFCRALILTHLHRDHISGVVALQNHLREKHDLHVPVAAH
                     KLTAKSVKDSIEVNRFIEDGEVFDLKISDAETLSLTALHTPGHARGHLCFYDARRGFL
                     LSGDNVLGAGSVLIAPPEGNMKDYLASLERMKNLPNLRFLCGSHGAAVFDAKSKIEEY
                     IAHRLEREKQILRALAGGAKNASEIAERVYPELDPKLFSLAVESVSAHLAKLYDEGVL
                     ESYFIKFER"
BASE COUNT         1897 a         1942 c         1893 g         2017 t
ORIGIN      
        1 ccgcatcgaa acaaaagcca tcaaaatcgc ctttggcgaa tacgcttaca aaatcccgat
       61 gagcgcgacc aaatcgcagc tcggtcatcc gcagggcgct tccggcgcgg ctggaatcgg
      121 cgcggcgctt ctcgccttaa acgaaaacat catcgcgccg acgataaatc tggatgagac
      181 ggacgaagcc tgcgatttgg attacgttcc gaacgcgtcg cgcgcggcaa aagtagaaac
      241 cgcgctctgc aactgcatcg gcttcggctc gaaaaattcg gctttgattt taggaaaagt
      301 taaataatcg tttaaaactc aacttgtatt aaaagctctt tagaaatcca tctaatccgc
      361 ttgtaaaata atccattttc ggaaaaaaag cgcggtaacg ctctgcttcg gcgtctgaaa
      421 tttggaaaac ttttactttc ggctgatttt tattggcgcg cattccgcga ataaacatcg
      481 agcggatggc taaatcttcc gacggcatcg aatatccgat aatggtgacg gagctcgcct
      541 ggctgatata ttcgatgccc gccagcacaa gttgttttag ctgcgcttcg gggtgaaatc
      601 tggcgagaga aggcgttatc aataagcttc tgagcggcgc atatccgcag tgacaggtga
      661 cctcgctgcc gtaattccgc gagtccgaaa aatctcccaa atccgctatc gcgtaaagaa
      721 gattgatgta gatttgttcg cacgaagggc atttcagcca atttaacgaa ccgtgtaatt
      781 tcagcagcgc ataggaatta ccgtcagccg gtcgtctgat gattttaccg gtcgtcacgc
      841 gtcgccagtc gaagccgtaa tcaagttctg ctgccggaat accagccttt tgaaaaacgc
      901 gctcgggaat taagtcgtaa ttacttgtaa taacaaccgt ctgtttaaaa cggcgcagca
      961 catccgcaaa cagccgaata ttttcattcg agtcaggctc gacatttaag acttcgatga
     1021 ttgcggcatc caccagatgg cgcaggcggc gtaattcgaa aaagccgtct ttaggcaaca
     1081 atgtgattcc ggacgaaata ctgaaatcaa tcagcgataa aacgtcggtg atacttaaat
     1141 ccctggcgct ggacgacaat cccggaatca gctgccggag agtctttttc aacaacgctc
     1201 tcttttcttt cgccctatca taatcggcaa aaagtgtttc gttggataaa ttttcaataa
     1261 tcttaggcaa aatttcatta ccgcctaaaa aacccagagc ttttgaagcg cccgcaccga
     1321 gaaaaataac attcataact acctatgtaa taaattaaac cgttttgccg cgacgttttt
     1381 ttgattcgct cgccgaatac tcatttcgtt aatttttgcc gggcattacc ggctgcgtcc
     1441 aggctatttt tccattcgat tcgattattt tagcgcgcac gtaagcctcg tctccgcgaa
     1501 attgataaac cgccggatta gtaatcgctt cgctcagtac gcgcccgcct ttgccgataa
     1561 actgcacgcg gtatttgctt gttctctctt cgttgatagt gacggcgatg tttttgtcgc
     1621 tgacttgata atcagccagt tcgactccgg tcgaggcata aaaatctcct ctctccattg
     1681 cttccagtat cgccgcgctc gaaagccgct ccgccctgac aaatatccag ccttgtccgg
     1741 gtcgcggcgc gcttttatcc cagggctttt tgaagacgtg cgcgtcgtcg acggcaattc
     1801 cgtaaagaag cttgccgccg gacaaaattg cgtcccacat ctcttccagt ccgggtttgc
     1861 cgccgccgcc gaaattgttg acctccgggt gaccgttgta gatttcaaaa agcttgttgt
     1921 tctccacttg tttcaggtcg tccgccgtta tcatccagcc gaaattcgga tgattgatgt
     1981 gcggaacgcc gcgcgcatcg cgaatggcat cgacgtttcg ctgaatgact tcgaccacgc
     2041 tttttccgcc ctgcggcttg accgcccgct cgacgttaag cccgttgacg tggattggtt
     2101 ttttgttgaa agtgtccgat acttcctcgc cgcgaataac gacgaatttt tcgtcggctc
     2161 cgtgcagagc gttaagtcct tcgacgttgg tcaaaaaatt gtggtcgctg agaacgagaa
     2221 aattatatcc gcgttcccga taccatcgca cgacctcgtc aggcgtcgaa tcgccgtcgc
     2281 tgttgatggt atgcgtgtgc gtgtttcctt tataccaatt caggcgtctc tggctcggcg
     2341 cgactttttg ctgcgcctga tgacccgcat taacggcgaa aatcgtataa agaaataata
     2401 atgaaaaaat agcggctaaa gatttttgca tcggaatatt aataaagcaa tttgcctgtc
     2461 gatgcaacaa aataactgat aactgataac tgataactga tagttttttg tttctttcac
     2521 tatcagttat cagttatcag ttataaaacg gttttcggcg caatcggttt tccgagttcg
     2581 ggctttctgc cgataattaa aaagcgctgc aaaagagtca gtttttcttc cggttctttc
     2641 ggaatctcga cgtattcgac aatgattttt tcgttttccg aacgaatctt aaaataattc
     2701 aaacgaaaag tcgggtaata aattccgggc agcaagccga aacgcaccga aacgcgcttt
     2761 aatattttca gccacgttcc ggtgttgata atgacgcgcc cttccggttc ctcgaccaga
     2821 aaagcgtcgt gcgtgtgtcc gaaaatataa gcgcacaccg ccggattttc cttgaaaatc
     2881 gttcgcgccc gatcgagata aatattgttc gcctcattgg tcggcgccga tttataggac
     2941 ggaaaaatct gcatccggaa aagcgtccgg cgaatatccc gataaacaaa ataaagcgga
     3001 atcgcaataa cgaaaacaaa aaaccagacc atcatactcg ccgtcaaaat ccagcgcaca
     3061 atgtcgcccg ccaagcccaa ccagcgaata aaagaatttt ccagcaaaat attgacatca
     3121 aaaatgccga gatatttgag catttgagcc aaaagcgcaa atattgtgac ggtcagcaaa
     3181 agcagaaacg gaagcaaaag ccagcgcaga aacacgttca tttcattgta gaaataattt
     3241 gagatgagcc agtcgggaat ctgccggaca tcgaccgagc ggatgtcttt gagccagtcg
     3301 cttgtgccga aaaccgaata tttgctcgcg ccgctgacca gagcctcggt tataaaatat
     3361 ccgagcggca gagcattttt atttccgtat tcggtcgccg cattatacgg gtcaatctgc
     3421 tgtccgtgtt cgacccagat ttttctgccg ccggtttcgc ggacgaccgc catcgaaagg
     3481 tcgagcgtga tgttgtagct tgccagcatc ggaacgaatt cgggatagca ggctaaatcg
     3541 taatcgtgat tgccgaccat cagcgtgatt tttattttct cgcccgtcgc ccgcagctgc
     3601 tcgaaaattc gcgtgtgatg cttgataatc tcctcgagct gcgccgttcc ttcgacgccg
     3661 gtcagctccc agaaaccgaa cgtatcgccc gcgataatta attcgatgtc tttgtcttcc
     3721 gccgccagct gctgcaaaaa cgcgataaat tcctcagtgt aatcgcaaat ctgcaattga
     3781 ccgtcgccgc cgaaatgcaa atcgctgacg aaataatatt ctgtggattc gctcttttcc
     3841 atcgtttcgc gcttcatagt ttatactatt gtatcgctta gatttcatat tttaacaatt
     3901 gataaaaatt aatgaacgta acaaatttat tttgctccgc ctgtgataaa acttacgcgc
     3961 cgaatcgcct ttacaatctc tgcgcggctt gcggcaagcc tttgctggtc gcttacgatt
     4021 tggaaaaagc cgccgctacc ttaacgaaag aagctttaaa atcgcgcatt ccgtcgctgt
     4081 ggcgatacga agaagttttg cccgtcgaaa acccggaaaa ccgcgtcacg ctcggcgaag
     4141 gaatgacgcc tctgcaccgg gcggaaaatt tagggcgaaa attaaattta aaaaatcttt
     4201 tcatcaagga cgaatccgcc aacccgaccg cttccttcaa ggcgcgcgga atgaccgccg
     4261 ccgtttcgat ggcgaaggaa ttaggcgtca aaaagctagc cgcgccgtcc gccggaaatg
     4321 ccgccggagc gctggcggct tacgcggcaa aagccgggat ggaagcgttt ttatttatgc
     4381 cgcgcgacac gccgcaagcc aacatcgtcg aatgcgagca gatgggcgca aatgtcacgc
     4441 tcgtcgacgg cttgattacc gattgcggaa aaatcgtcgc cgaaagaaaa gcggctgaag
     4501 gctggtttga cgtctcgacc ctgaaagagc cgtatcgcat cgagggcaaa aaaacgatgg
     4561 gttacgagat tgccgagcag atgaattttg aattgcccga cgtaattttt tatccgaccg
     4621 gcggcggaac ggggttgata ggaatgtgga aagcctttga cgaaatggaa aagctcggct
     4681 ggatcggcga caaacgcccg cggatgattt cggttcaggc agccggctgc gcgccgattg
     4741 tcgaaggctt cgagcaacac ttaaatcacg gcgcggaaat cgaaaacgct cataccgtag
     4801 cttccggctt gcgcgttccg aaagccatcg gcgattttat cattctcgaa atcgtgcgaa
     4861 aaagcggcgg aagcgccatc gccgtcacgg acgaagaatt gatttcggac gtcagggaaa
     4921 tcggcgcggg cgaaggcatt tttcccgcgc cggaaggcgc cgcctgcttg tccgcgctca
     4981 aaaaaatgat tgccaccggg acaatcgaaa gcgacgagca catcgtttta ttcaacacgg
     5041 gctcgggcgt taaatatctc gaagcgtttc aagcctgaaa aatgagcgat gaaaatttat
     5101 gagttttaaa tgttaaattt tgaatctgca atcaaacgaa tcattcaaaa ctcaaaactt
     5161 aaagcctaaa gctttctttc ttcagccgtc aaaaaaagac gcggcgacaa acaggttcca
     5221 ttatcaccgt tatttatacg gcaaattgtg aaaagcagga ttatttttta gatttaccgc
     5281 cttttttcgg cgcttttttg ccgccggtaa ctcccacggc ttccgctgcg cctttgaccg
     5341 cttcggttgc cgcgccgaca acttcgccgg ctactttttg agctttgccg attatgccgc
     5401 tatcggcttt ttttttggct gcgggtttcg ccgcagtttt cgctgccggt ttggcggctg
     5461 ccgttttagc cggagctttc gccgccggtt tggcggcgtt ttttattgtc gcgctcttag
     5521 ctgccggttt tgccgcagtt gccgctgctt tcttcgccgg agccttggcg gcggatgtgg
     5581 ttttcttagc cgtcggagcc ttcgctgccg ccggcttagc ggcggtgttc ttggcggcgt
     5641 tcgattttgc tgccggcgtc ttgccgtcgg gtttagccgc tgcttttgcc gctggagctt
     5701 tcgtcgccgt tttgtcaggc gcggctttct tagcgcccgt tttactcgat gctttagttg
     5761 ttgccatttt ttctcctttg attttcggaa tagaaattac tccgcaaaga atttaacacg
     5821 atttttatat aaaaaatcaa ggtttttttc acttcttcag gcaatacgcc gatttttttt
     5881 tgttgattag ttgtggttgt tttaaaaggc ggcacaatat aatccacaat tcgatgtttt
     5941 atgaaaaatt tttacgtcct ctataaatct gaaaatcggc tcgcccgaac cggattggcg
     6001 ggattttgcc ctttatcaaa agacggcgct cgattgtttt aaccttttag acgcttgagt
     6061 taaagtgttt taaaggcagt aatttttata atgtttcaga agcgaagcac aaaactcgag
     6121 ctcatcgata cgggcgatta cacggcgcag gaatacgagg gctgcctgcg cgagctgcga
     6181 cgcgtaaatc attatctagg cgatacctgg gctttgcgga aaacgctttt gcgcgaaatc
     6241 gaaaataaaa atccgccgca attttccgtg ctggatgtcg gcgccggttc gggcgagctg
     6301 ctgcgggcga ttgccgaacg ggcgaaaggc gagagaagcg cgcgtcttgt cggtttggag
     6361 cttaacgcgc gttcggcgcg ggcgattctg gaagaatcaa aaggctttgc ggaaattttt
     6421 tcgattcgcg ccgacgctct gcgtttgccg tttgccgacg cgtcgttcga ctacgcgatt
     6481 tgttcgcttt tcacgcatca ttttacggat gaaaaagtcg tccggattct gtcggaaatg
     6541 aagcgcgtcg ctcgccggaa aatttttgtc atcgaccttc accgacacgc tctggcgtat
     6601 tttctatata ccactgcggg caaacttttc ctttataatc gactcatccg gcacgacggc
     6661 gcgctctcga ttttgcgctc tttcaaaccg gatgaattaa aggatttagc ggcggcggca
     6721 aaactggaaa acgtctcggt ggaaagacat tttccgtttc ggctggtctt aagcggcagt
     6781 tgaagtcggc gatttttcgc gacggtcggc taatctgtcc ggaatgccgg aatattgcct
     6841 ttgttaaaat cctcacagat tgcttggagc gtcgccggat tgctcataaa tcatctctcg
     6901 aatttaataa aatacgactc taaaacacct tcgtcgtata atttcgcgag atgcgcggag
     6961 acggattcga cggcgagcga aaaaagcttc gggtcgagtt ccggataaac gcgctcggca
     7021 atttcggaag cgtttttcgc gccgccagcc agcgcccgca aaatctgttt ttctctttcc
     7081 agacgatgcg cgatgtattc ttcgattttc gatttcgcat cgaacaccgc cgcgccgtgc
     7141 gagccgcata agaatctgag attcggcaga tttttcattc tttcgagcga agccagataa
     7201 tctttcatat tgccttcggg cggcgcgatt aaaaccgagc ccgcaccgag aacgttatcg
     7261 cccgacagca gaaatccgcg ccgcgcgtcg tagaaacaaa gatgcccgcg cgcgtgtccg
     7321 ggcgtgtgca gcgccgttaa gctcagcgtt tcggcgtcgc ttattttcaa atcgaaaact
     7381 tcgccgtctt caataaaacg attgacttcg attgaatctt tcacgctttt cgccgtcagc
     7441 ttgtgagccg cgaccggaac gtgcaaatcg tgtttttcgc gcaggtgatt ttgcaacgcg
     7501 acgacgcccg aaatgtggtc gcgatgcaga tgcgtgagaa taagcgcgcg gcaaaaattc
     7561 ccgttttcaa tcaaagaatc tatgtattcg tgcagcgcgg actgttcttc gacaaagggc
     7621 gaagcggcat cgacgacgac aaactcgcgc tcgccggcga taaaacaatt ggtgtgcgtc
     7681 gcgggcggca gagtttcggt gcgaacggga aaacaagcaa tatacggatg aaatctgacg
     7741 cggcgaatt
//
LOCUS       DASWNE010000644         4939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_270217, whole genome shotgun sequence.
ACCESSION   DASWNE010000644 DASWNE010000000
VERSION     DASWNE010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270217"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..593
                     /locus_tag="VGB68_12650"
     CDS             138..593
                     /locus_tag="VGB68_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050135.1"
                     /translation="MKHFLNVTIVLLFSLILAGCAEKQPTNATIYQVKLSPKIQGAEE
                     KVFTKEVTTGFEDNPQPLKITLTSFLNDEACWKTAEIKVDRNGGDENVVVSITNHDIV
                     PPCALDMESASDQRFDTVSINTSFKYGLLGSGGKGAVFIRSDGNPAVTR"
     gene            851..1480
                     /locus_tag="VGB68_12655"
     CDS             851..1480
                     /locus_tag="VGB68_12655"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR00421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HEY0050136.1"
                     /translation="MELTVALTGASGTIYAYRTLQLLAESGAVSQVNLIMSGTSATVA
                     QVETGVNLREPDAEKINQWLGLAKDSKFIRFWRLDNLAAKPASGSYRQEGMIIVPCSM
                     GTLGAIASGAGANLIHRAADVTLKEGRKLVLVPRETPYNAIHLENMLRLARAGARILP
                     ASPGFYHRPQTINELVDHLCYRILDQFGIPHSKKSQWTGDEVPQQENKR"
     gene            1477..2181
                     /locus_tag="VGB68_12660"
     CDS             1477..2181
                     /locus_tag="VGB68_12660"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEY0050137.1"
                     /translation="MKILENRKIFFTFFLLIFAVSGLLAQERQERKVLPVDEGKTDAS
                     FSAFREKTLEAARRRDAKYILSIVEPNIKNTFGDSNGIEEFKKMWKLTSPQSEFWKEF
                     VTVLANGGAFWKRPGAPKKQIFQAPYTFTEFPADLDSFEYQSIFGSSVNLRSKPEPGA
                     PVVAALSYNVVKVDFENSVKDETREGKYLWLKVETLGGKKGFVNAGYVRSPVDYRAVF
                     EKRNGRWKLVTFIAGD"
     gene            2188..2943
                     /locus_tag="VGB68_12665"
     CDS             2188..2943
                     /locus_tag="VGB68_12665"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0050138.1"
                     /translation="MANTVERFTNRVENYVKYRPTYPPEVLRFLQNELNLQIDSVIAD
                     VGSGTGISAKIFLENGNTVYGVEPNAAMRAAAEDFLKNFPNFRSVDGTSENTTLSDQS
                     IDFVTAAQAFHWFDAEKTRKEFKRILKPGGSVVLIWNERRIDTNEFLREYEKFLLQFG
                     TDYAKVRHDYLPQERLGEFFPDGFQTAAFQNSQIFDFAGLKGRALSSSYMPSERDARF
                     EEMTKSLYGIFTKHQENDRITVLYDTNIYYGKL"
     gene            2958..3809
                     /locus_tag="VGB68_12670"
     CDS             2958..3809
                     /locus_tag="VGB68_12670"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HEY0050139.1"
                     /translation="MEAKAETRTISVAHSPDSDDAFMFYGLATNKLDTGNLKFEHTLK
                     DIQTLNENAKSGVYDVTAISFHAYAYVSDKYALLPHGASIGDKYGPILVSKEPRKAED
                     ISKMKIAVPGELTSAFLALRIYNQDFEYTIVPFDQIIDEVKRGKADAGLLIHEGQLFY
                     KQVGLDKVLDLGEWWHEKTGLPLPMGGNVIRRDLGEDLMRQVSKYLRQSIQYSMQNRE
                     DALAYAMQFARDMQPELADRFVAMWVNDLTLDYGERGREGVRRLLEEGFEKGIIPNRV
                     EIEFVEG"
     gene            3975..4802
                     /locus_tag="VGB68_12675"
     CDS             3975..4802
                     /locus_tag="VGB68_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050140.1"
                     /translation="MKKFKFFLLTAIVCLAAFGNAAAQDAITKNNLSQAEIDRIVKNF
                     TTKEGEFRAALTQYVFNRSASISTIGLGGNITGTYRRDSFMSITPEGERREKILFAPI
                     STLTEISVTPEDLEDLGGVNPFALEPSVVNLYNFTYVGKEKIDELDLYVFDVAPKVMP
                     DPKKTKQRFFIGRIWVDDRDLQMVKSKGKAIPETKQNKFPVVETLRINVDGKYWFPAD
                     SRSDDELVFDNGNVVKMKVRVKYTNYRQGRSDVKILDDEEEVKEDTKPAPTPTPKKP"
BASE COUNT         1388 a         1146 c         1243 g         1162 t
ORIGIN      
        1 attcgacttg atatattgtg gtttcaaaaa cgaccatttt cgctacttga aattagtaat
       61 aatcgaaaat gcattcaggc agctttctga aaaaatggaa agtatctata atcgccgaaa
      121 agctaacgag gtaaaatatg aaacattttc tgaatgtaac gattgttctt ttatttagtt
      181 taatcttagc gggctgcgct gaaaagcagc cgacaaacgc tactatttac caagtcaaac
      241 tttccccaaa gatacaggga gccgaggaaa aggtctttac taaggaagtt actacgggat
      301 ttgaagataa tccccaacca ttaaaaatta ccttgacatc atttttgaac gatgaagcgt
      361 gttggaaaac agccgagatt aaggttgaca gaaacggcgg cgacgaaaat gtcgtagtta
      421 gtatcacaaa tcacgacatt gttccgccgt gcgctttgga catggagtct gcgagcgacc
      481 agcgtttcga tacggtttca ataaatacca gcttcaaata cggtttgctc gggtcaggcg
      541 gcaagggcgc agtgtttatc agaagcgacg gcaatccggc ggtaactcga taactttcag
      601 attaaaaaga ccttctttaa tcttttcgcc ggtcaggcgc ggttctggag cgtattaaaa
      661 atcaagtgta aatccaggtt ttcgcagcaa gacaatttac acttgatttt ttcgcgccta
      721 tttttgaagc gcaaatttcg tttcgggaaa cctgaataat aactgttttt cgtcagcccg
      781 aatcaggacg ccgctaaagt tttgttttat ttgtgatatt cccgaaaaaa agtgtagagt
      841 aaaaatctat atggaattga cagtcgccct gacgggcgca tcgggcacga tttacgctta
      901 ccgcaccttg cagcttttgg cggaaagcgg cgcggtctcg caggtgaatt tgataatgtc
      961 gggcacgtcc gcaacggtcg cgcaggtcga aacgggcgtt aatttgcgcg agccggacgc
     1021 cgaaaaaatc aaccaatggc tcggcttggc aaaagactcg aaatttattc gcttctggcg
     1081 gctcgacaat ctggcggcga agccggcgtc cggttcttat cgtcaggagg gaatgataat
     1141 cgtgccgtgt tcgatgggaa cgctcggcgc aatcgcttcg ggcgcgggcg cgaacctgat
     1201 acatcgcgcg gctgacgtga ctttaaaaga aggcagaaag ctggttttag ttccgcgcga
     1261 gacgccgtat aacgcgattc atctggaaaa tatgctgcgc ctggcgcgcg ccggagcgcg
     1321 gattttgccc gccagtccgg gtttttatca tcgtccgcag acgattaatg aattggtcga
     1381 ccatctgtgt taccgcattt tagaccagtt cggaattccg cattcgaaaa aatcgcaatg
     1441 gacgggcgac gaagttccgc agcaggagaa caagcgatga aaattctgga aaaccggaaa
     1501 atatttttta ctttttttct tttaatcttc gccgtttccg gcttgctcgc gcaggaaaga
     1561 caggaaagaa aagtgcttcc cgtcgacgaa ggcaaaacgg acgcttcatt tagcgctttc
     1621 cgcgaaaaga ctttagaagc ggcgcgacgg cgcgacgcga aatacatttt gagcatcgtc
     1681 gagccgaata tcaaaaacac tttcggcgac agcaacggaa tagaagaatt caaaaaaatg
     1741 tggaagctga cgagcccgca gagcgagttc tggaaggaat tcgtcaccgt tctagccaac
     1801 ggcggcgcgt tctggaagcg accgggcgcg ccgaaaaagc agatttttca agcgccatac
     1861 acgtttaccg aatttcccgc cgatctggac agctttgaat atcaatccat tttcggcagc
     1921 agcgtaaatt taaggagcaa accggagccc ggcgcgccgg tcgtcgccgc tctttcttac
     1981 aacgtcgtca aagtcgattt tgaaaattcg gtcaaagacg aaacgcgcga aggcaaatat
     2041 ctatggctga aagtcgaaac gctcggcggc aaaaaaggtt tcgtcaacgc cggatacgtt
     2101 cgcagtcccg tcgattatcg cgccgttttt gaaaagcgaa acggcagatg gaaactggtg
     2161 accttcatcg ccggagatta aaagtttatg gcaaacacgg tcgaaagatt cacgaaccgc
     2221 gtcgaaaatt acgtcaaata ccgcccgacg tatccgccgg aagttttgcg gtttttgcaa
     2281 aatgaattaa atctgcaaat tgattcggtc atcgccgacg tcggttcggg cacgggaatt
     2341 tccgcgaaaa tttttctcga aaacggcaat acggtttacg gcgtcgagcc gaacgcggcg
     2401 atgcgcgcgg cggcggaaga ttttttaaaa aattttccga atttcagaag cgtcgacgga
     2461 acttcggaaa acactacgct ttccgaccaa tcaattgatt tcgtcacggc ggcgcaggcg
     2521 tttcactggt tcgacgcgga aaagacgcgg aaagaattta agcggatttt aaagcccggc
     2581 ggctcggtcg tcctgatttg gaacgagcgc cggattgata cgaacgaatt tctgcgcgag
     2641 tacgaaaaat ttcttttgca gttcggaacc gattacgcga aagtgcgcca cgattatctg
     2701 ccgcaggaaa gactcggcga atttttcccg gacgggtttc agaccgccgc ttttcagaac
     2761 tcgcagattt tcgactttgc gggcttaaaa ggcagggcgc tttcttcgtc ctatatgccg
     2821 agcgaacggg acgcgcgttt tgaggaaatg acaaaatcgc tctacggcat ttttaccaaa
     2881 caccaagaaa acgatagaat aacagtttta tacgacacca acatctatta cggaaaactt
     2941 taaaagaaga tttatttatg gaagcaaaag cagaaaccag aaccatcagc gtcgcgcatt
     3001 caccggattc ggacgacgcg tttatgtttt acggtctggc gacaaacaag ctcgacacgg
     3061 gcaatctgaa atttgagcac accttaaaag atattcaaac cttaaacgaa aacgcgaaat
     3121 cgggcgttta tgacgtgacg gcaatcagct ttcacgctta cgcctatgta tcggacaaat
     3181 acgcgctgct gccgcacggc gcgagcatcg gcgacaaata cggtccgatt ctcgtttcca
     3241 aagagccgcg caaagccgaa gacatctcga aaatgaaaat cgccgtgccg ggcgaattga
     3301 ccagcgcttt tctggcactc aggatttaca atcaggattt tgaatataca atcgtcccgt
     3361 ttgaccaaat catcgacgag gtaaaacgcg gaaaggcgga cgccgggctc ttgattcacg
     3421 aaggacagct tttttacaaa caggtcgggc tcgacaaagt tctcgatttg ggcgaatggt
     3481 ggcacgagaa aaccggcttg ccgctgccga tgggcggcaa cgtcatccgg cgcgatctgg
     3541 gcgaggattt gatgcgtcag gtttccaaat acctgcggca gagcattcag tattcgatgc
     3601 agaaccgcga ggacgcgctc gcctacgcga tgcagtttgc gcgcgatatg cagccggagc
     3661 ttgccgaccg ctttgtggcg atgtgggtca acgatttgac gctcgattac ggcgagcgcg
     3721 ggcgcgaagg cgtgcgccgc ctgcttgagg aaggcttcga gaaaggaatt attccgaacc
     3781 gcgtcgaaat cgaatttgtc gaaggttaag ttttaaaaac gcaaaacgca aagtgcaaaa
     3841 tattaggaat tagaatttaa aattaaattt cttcttccat tttgcacttt gcattttgcg
     3901 ttttgcatta atatacgcat cttttcacgg atttccggca tctatttttt aacttctatt
     3961 caagaggaaa aacgatgaaa aagttcaaat tctttttatt gacggcgatt gtttgtctgg
     4021 cggctttcgg aaatgcggcg gcgcaggacg caatcaccaa aaacaatctc agccaggcgg
     4081 aaatcgaccg catcgtcaaa aactttacga ccaaagaagg cgagtttcgc gcggcgctga
     4141 cgcagtatgt ttttaatcgc agcgcgtcga tttcgaccat cgggctgggc ggaaatatta
     4201 cgggcacgta tcgccgcgat tcttttatga gcattacgcc ggaaggcgag cgccgcgaaa
     4261 aaattctttt cgcgccgatt tccaccttga ccgaaatcag cgtgacgccc gaagatttgg
     4321 aagatttggg cggcgtcaat cctttcgcgc tggagccttc ggtcgttaat ctttacaact
     4381 ttacatacgt cggcaaggaa aaaatcgacg agctcgattt atatgttttc gacgtcgcgc
     4441 cgaaggtgat gcccgacccg aaaaaaacca aacagcgttt tttcatcggc agaatctggg
     4501 tcgacgaccg ggatttgcaa atggttaaat ccaaaggcaa agccattccc gaaaccaagc
     4561 aaaacaaatt tccggtcgtc gaaaccctgc gcatcaacgt tgacggaaaa tactggtttc
     4621 ccgccgattc gcgctcggac gacgagctgg ttttcgacaa cgggaatgtc gtgaaaatga
     4681 aagtccgcgt caaatacaca aattaccggc agggacgctc ggacgtgaaa attctcgacg
     4741 acgaagaaga ggtgaaggaa gatacaaaac ccgcgccgac accgacgccg aaaaaaccgt
     4801 aaaataactg ataaccggta gtggataacc gatactaaac cactctgcac tgaaagtgca
     4861 gaggtttaag aatcggaacg caaatagaat ttgcttgatt taatcttttc agacaaagaa
     4921 aagaaagcat aataaattc
//
LOCUS       DASWNE010000645         1542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_57641, whole genome shotgun sequence.
ACCESSION   DASWNE010000645 DASWNE010000000
VERSION     DASWNE010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57641"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..1470
                     /locus_tag="VGB68_12680"
     CDS             280..1470
                     /locus_tag="VGB68_12680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEY0050141.1"
                     /translation="MSEPITTPVNVSSNNSVKAPSPQMTSPNPAISQNTQAFHGVLHR
                     MLSVDPKISDLIFSPGRPPQVELTGDLQGVQIPGLERLTPPQIKAMADLMLHGNEQGL
                     ETLDKKGSADISYSVNGLCRFRVNIFRQRGTYAIVMRVIPTKPPNWEELNLPRAVMNA
                     AELKNGLVLVTGPTGSGKSTTLAAIIDLINSTKKYHIVTIEDPIEFIHEHKLGTVHQR
                     ELHSDTPDFALALRAALRQAPKVILVGEMRDRETIEVAMEAAETGHLVLSTLHTVDAA
                     KTIDRIIGVFPKVEESAIRTRLSQSFRRIISQRLMPKAGGGRIAAIEILVSNSRTREY
                     IEKGEKEGRSIVDAMNDGEIEGMQTFDRVLENFIREGLVRKEVAVAYATNQNNLLLSI
                     SDLG"
BASE COUNT          393 a          419 c          395 g          335 t
ORIGIN      
        1 gttttgccgc agttgcggca caaatagcgt tgtgtgccgc aatgagtttt cccgcttttt
       61 atctggtttt ctgaattttc acaatttgaa caagttggca ttgagaaagt ttatcttaac
      121 aacacttatt tagtcactcc caggattaca aagtcattga ttaattagcg gagaagatag
      181 taatattaaa ttgtcgaatc cgcagcgatt gacgccagcc tgtaataacg aacccgattt
      241 cttactttct acattcaacc aaagtaacaa taaaacaaaa tgagcgagcc gataacaact
      301 cccgtcaatg tttcttcaaa taattccgtc aaagcgcctt ctccgcaaat gaccagcccg
      361 aatccggcga tttcgcagaa cacgcaggcg tttcacggcg tcctgcaccg gatgctgagc
      421 gtcgacccga agataagcga tttaatattt tcgcccggtc gcccgccgca ggtcgagctg
      481 accggcgatt tgcagggcgt ccagattccc ggtctggaac gcctgacgcc gccgcaaatc
      541 aaggcgatgg ctgacctgat gctgcacggc aacgagcagg gcttggaaac gctggacaaa
      601 aaaggctccg ccgatatttc ctacagcgtc aacggcttgt gccgctttcg cgtcaatatt
      661 tttcgccagc gcggaaccta cgccatcgtg atgcgcgtca ttccgaccaa gccgccgaac
      721 tgggaagaac tgaatctgcc gcgcgccgtg atgaacgcgg cggaattgaa aaacggcttg
      781 gttctggtga cgggtccgac cggctctgga aaatcgacga cgctggcggc gattatcgac
      841 cttatcaact cgaccaaaaa atatcacatc gtcaccatcg aagacccgat agaatttatt
      901 cacgagcaca agctcggcac ggttcaccag cgcgagctgc actcggacac gcccgatttc
      961 gcgctcgccc tgcgcgccgc tttaaggcaa gcgccgaaag tgattctggt cggcgagatg
     1021 cgcgaccgcg aaaccatcga ggtggcgatg gaagccgccg aaaccggaca tctcgttttg
     1081 tccactttgc acacggtcga cgccgccaaa accatcgacc gcatcatcgg cgttttcccg
     1141 aaagtcgaag aatcggctat cagaacgcgc ctttcgcaat cgtttcgccg cattatttcg
     1201 cagcgcttga tgccgaaagc cggcggcggt cgaatcgccg ccatcgaaat tctggtgtcg
     1261 aattcgcgca cgcgtgaata tatcgaaaaa ggcgaaaagg aagggcgctc gattgtcgac
     1321 gcaatgaacg acggggaaat cgaaggaatg cagactttcg accgcgtgct cgaaaacttt
     1381 atccgcgaag gtctggtcag aaaagaagtc gccgtagctt acgcgacgaa ccagaataat
     1441 cttttgcttt ccatcagcga tttggggtaa cgcacgccgg aaatcgaatt gaaaaacggc
     1501 ttggttctgg tgacgggtcc gaccggctct ggaaaatcga cg
//
LOCUS       DASWNE010000646         3652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_22471, whole genome shotgun sequence.
ACCESSION   DASWNE010000646 DASWNE010000000
VERSION     DASWNE010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22471"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2406)
                     /locus_tag="VGB68_12685"
     CDS             complement(<1..2406)
                     /locus_tag="VGB68_12685"
                     /inference="COORDINATES: protein motif:HMM:NF025253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEY0050142.1"
                     /translation="MKLHTVLILLVLLFVAAGSAFGQTSVISYQGRLAEASAVENGEY
                     QFQFKLFDAAQGGNQIGSIVEVRTLVTNGAFSAGLDFGEKAFNGEDRYLEISIRKTES
                     DAYVPLTSAREKVNSVPYAIKANTATTAETVAGMTSTDISGSVQTVNNATPNNVGGAI
                     VKRDDQGQIAVSGVIFPDGTVLKSGSFTGGKVVNQTVDNIGGTGTTNRISKWIDGSGT
                     LGDSTISENSQGLVGIGNPNPTAKLSVLDGANAELRFAQGASGITPSLSVISTPSSMT
                     TGGGLTLATGTGGSSFVFSDNLPFYIVKDTKANVVNNNLGHGTLLFTVLPNGNVGAGV
                     VNPTERFEANGNIKISGEGSRLIFPDGTFMTTAGSGGGGNMTGTSIITAVNDASTSGL
                     ISDNRVSTNLARLTGANVWSAANVFSAGLSANNSLITNVGNPVNAGDAVNKIYADSNF
                     VKFVPGAEQLSVGDANGTAPMINLRGGSTCCSSPGGHTPAWFKVFQNGSFVATGNLGI
                     GVSPMQGKGYRTSWDSYKGAFRSGYADNEWDDAKVGFFSWAGGSNSTAEGLYALAFGD
                     TNFARSTSSIAFGSGNEVKGAAGFSAGAGNRVCDTYGVALGNNAKSGGPLINGKCDPD
                     TFNIRGLAAFALGYYVTADQDHSFALGKFATNNGFSGTFIWSDGSAQASADTFRNTAN
                     NEVAFRATGGFRFRTNLGGTTGCNLPAGSGVFNCTSSRFTKENFLKVEGFDVLSRLRK
                     IPVTSWNYISEGQQVRHIGPMAEDFYEQFKLGTGNTSIGVQDLAGVSIAAVKELDAQL
                     QEKN"
     gene            2654..2815
                     /locus_tag="VGB68_12690"
     CDS             2654..2815
                     /locus_tag="VGB68_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050143.1"
                     /translation="MKNAFAIVKITIGSVTIAVGSVTVGIAKLKIALGGQNSLLAERQ
                     ALSTRKNRF"
     gene            2928..>3652
                     /gene="mdh"
                     /locus_tag="VGB68_12695"
     CDS             2928..>3652
                     /gene="mdh"
                     /locus_tag="VGB68_12695"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEY0050144.1"
                     /translation="MLRRNKVTVVGAGNVGATAAHWIASKELADVVLVDIVEGTPQGK
                     SLDLAQAAPIDGFDVKLVGANSYEETADSDVVIITAGLPRKPGMSRDDLLKTNSDIVG
                     NVTDQVAKYSPDAFIIVVSNPLDAMTQVAFKRSGFPKNRVMGMAGVLDAARMRCFLAE
                     ALNVSVENVTAFVLGGHGDTMVPLPRYSTCAGIPITELLPKEQIDAIVKRTANGGAEI
                     VGLLKTGSAYYAPSLGAVEMAEA"
BASE COUNT          820 a         1013 c         1001 g          818 t
ORIGIN      
        1 atttttctcc tgcaactgcg cgtccaattc cttgaccgcc gcgatgctca cgcccgccaa
       61 atcctgcacg ccgatcgaag tgttgccggt tccaagcttg aactgctcgt agaaatcttc
      121 agccatcgga ccgatgtggc gaacctgctg accttccgag atatagttcc acgaagtaac
      181 cggaatcttg cggagtcttg aaagaacgtc gaagccttcg actttcaaaa agttttcttt
      241 cgtaaagcgg ctcgatgtgc agttgaaaac gcccgaacca gccggaagat tacagcccgt
      301 cgtgccgccg agattcgtgc ggaagcggaa gccgccggtg gcgcgaaaag cgacttcgtt
      361 gttcgcagtg tttctgaaag tgtcagccga agcctgcgcc gaaccgtcgc tccagataaa
      421 cgtgccgctg aaaccgttgt tcgtggcgaa tttgccgagc gcgaacgagt ggtcctggtc
      481 agccgtcacg tagtaaccga gcgcgaaagc agccagaccg cgaatgttga aagtgtcggg
      541 gtcgcatttg ccgttgatga gcggaccgcc cgacttggca ttgttgccga gcgcgacgcc
      601 gtaggtatcg caaacacggt tgcccgcgcc agccgaaaat ccagccgcgc ctttaacttc
      661 gttgccgctg ccgaaagcga tgctgctcgt gctgcgggca aagttcgtgt cgccgaacgc
      721 gagagcgtag agcccttcag ccgtcgagtt gctgccgcca gcccacgaga agaaaccgac
      781 cttcgcatca tcccattcgt tgtcggcata acccgagcgg aaagcgcctt tataagaatc
      841 ccaggaggtg cgatagcctt tgccctgcat cggcgaaacg ccgattccca ggtttccggt
      901 cgcgacgaaa ctaccgttct ggaaaacttt gaaccaagcc ggcgtgtgtc cgcccgggct
      961 gctgcagcag gtcgagccgc cgcgcaggtt aatcatcgga gccgtgccgt tggcgtcgcc
     1021 gaccgaaagc tgttccgcgc cgggaacgaa tttcacgaag tttgaatcgg cgtagatttt
     1081 attgaccgcg tcgccggcgt tgaccgggtt gccgacattg gtgataagcg agttgtttgc
     1141 cgacaagccg gcgctgaaaa cgttcgccgc agaccagacg ttcgcgccgg tcaggcgggc
     1201 gagattggtc gaaacgcggt tatcgctgat caagccgctc gtcgaagcgt cgttgaccgc
     1261 cgtgatgatg ctcgtgccgg tcatattgcc gccgccgccg cttccggcag tcgtcataaa
     1321 cgttccgtcc ggaaagataa gcctgctgcc ctcgccgctg attttgatgt tgccgttggc
     1381 ttcgaatctt tcggtcggat tgactacgcc cgcgccgaca tttccgttcg gcagaaccgt
     1441 gaaaagcaga gttccgtgtc cgagattgtt gttgacgacg ttcgccttcg tatctttaac
     1501 gatataaaac ggcaggttgt cgctgaaaac gaagctcgaa ccgcccgtgc cggtggcgag
     1561 cgtcaaaccg ccgcccgtcg tcatcgaaga cggcgtggaa atcacgctca ggctcggcgt
     1621 aatgcccgac gcgccttgtg caaaccgcag ttccgcatta gcaccgtcga gaaccgacaa
     1681 tttagccgtc gggttcgggt tgccgatgcc gaccaagccc tgcgaatttt cgctaatcgt
     1741 cgaatcgccg agcgttccgc ttccgtctat ccactttgaa atccggttcg tcgtgccggt
     1801 tccgccgata ttatccacag tttgattgac cactttaccg ccggtaaagc tgcccgattt
     1861 caaaaccgtg ccgtccggga aaatgacgcc gctgaccgca atctgtccct ggtcgtcacg
     1921 tttgacaatc gcgccgccga cattgttcgg ggtcgcgtta tttaccgtct gaacgctgcc
     1981 ggaaatgtcg gtcgaagtca ttccggcaac cgtttcggcg gtcgtcgccg tgttcgcttt
     2041 aatcgcgtaa ggaacggagt tgactttttc gcgcgcgctc gtcaaaggca cgtaagcatc
     2101 gctctcagtt ttgcggatgc tgatttccag ataacggtct tcgccgttga aagccttctc
     2161 gccgaaatcc agaccggcgg agaaagctcc gttcgtcacc agcgttctga cttcgacaat
     2221 cgagccaatc tgattgccgc cctgcgccgc atcgaaaagc ttgaactgaa actggtattc
     2281 gccgttttcg acggcgctcg cttcggctaa gcgtccctga taactaatga cggaagtctg
     2341 cccgaaagcc gaacccgccg cgacaaataa cagaaccagt aaaattaaga cggtatgtaa
     2401 tttcatgaat tttctcccaa acagtatgaa cttgtataaa tatgttcgtc ctcaaacgag
     2461 gcgcttctat taactaacgc gtaaaggaat tgaaaaaggt gtcagcccgg aaaaaaaaat
     2521 ttttgattcg gataaaacgg ataagttttc gtaaaaatcg cacgcatcaa attgaaaatc
     2581 gtgattgccg acataaaatc gctgttgccg ctgtgaaaat cacgattgcc gctgtgaata
     2641 tcgctattgc cggatgaaaa acgccttcgc cattgtgaaa atcacgattg gcagcgtgac
     2701 aatcgccgtt ggcagcgtga cagtcggtat cgcgaaactg aaaatcgcgc ttggcggtca
     2761 aaattcgctt ttggcggagc gtcaagcgct ttcgacgcga aaaaatcgct tttgaccttc
     2821 acgttttgga taaatttttg agaaaaaccg aatttgccgt ttgccttcaa ccgtgacaaa
     2881 ataacttttt atcaaacaca attaatttca aacagagagg aataaagatg cttagacgta
     2941 ataaagtgac ggtcgtcggc gcgggcaatg tcggcgcgac cgccgcgcat tggattgcca
     3001 gcaaagaact ggcggatgtg gttctggttg atattgtcga gggaacgccg cagggaaaat
     3061 ctctggattt ggcgcaagcc gctccgattg acggttttga cgtaaagctc gtcggcgcga
     3121 attcgtatga agagacagcc gattccgatg tggttatcat caccgccgga cttccgcgca
     3181 aacccggaat gagccgcgac gaccttctga aaaccaattc ggacatcgtc ggcaacgtca
     3241 ccgaccaggt tgcgaaatat tcgccggacg catttatcat tgtcgtttcc aacccgctcg
     3301 atgcgatgac gcaggttgcg tttaaacgtt ccggttttcc gaaaaaccgc gtgatgggaa
     3361 tggctggcgt gctggatgcg gcgcggatgc gctgttttct ggcggaagct ttaaacgtct
     3421 cggtcgaaaa cgtgacggct ttcgtcctcg gcggacacgg cgacacgatg gttcccttgc
     3481 cgcgctattc gacctgcgcc ggcatcccga tcaccgagct gctgccgaaa gaacagattg
     3541 acgccatcgt caagcgcacg gcgaacggcg gcgcggaaat cgtcggcttg ctgaaaaccg
     3601 gctcggctta ttacgccccc agcctgggcg cggtcgaaat ggcggaagcg at
//
LOCUS       DASWNE010000647         1506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_270315, whole genome shotgun sequence.
ACCESSION   DASWNE010000647 DASWNE010000000
VERSION     DASWNE010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270315"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..483)
                     /gene="sodX"
                     /locus_tag="VGB68_12700"
     CDS             complement(142..483)
                     /gene="sodX"
                     /locus_tag="VGB68_12700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-type superoxide dismutase maturation
                     protease"
                     /protein_id="HEY0050145.1"
                     /translation="MKNELPADDWREQLAYLCGFREIFLVQGDSMLPGLKDGDVVLVD
                     PHAALRIGDVVLARHPFKKSVQIIKRIREISPEGRYFLVGDNEDESTDSRSFGAIPAK
                     DILGKAVSRLK"
     gene            complement(552..1019)
                     /gene="sodN"
                     /locus_tag="VGB68_12705"
     CDS             complement(552..1019)
                     /gene="sodN"
                     /locus_tag="VGB68_12705"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HEY0050146.1"
                     /translation="MFEKFKEKLTANPAIAEAHCDAPCGVYDPAQTRVEAESVLQLTK
                     KILDLKRPEGDDATAHAAYQNTLTRFITIKETRADLAKHHLLVLWTDYFKPQHLEKYP
                     ELHELFWKTAKLCSACKQEISLEHAQELMDNIKRVHEIFWETKGKDVPWYTAS"
     gene            complement(1056..1388)
                     /locus_tag="VGB68_12710"
     CDS             complement(1056..1388)
                     /locus_tag="VGB68_12710"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY0050147.1"
                     /translation="MPKITAEAAQGTLAFEVEEGRKLVLCLEDNGIDILHRCGGNARC
                     TTCRVEVLAGDPGEIGEPEKAILATKTDLSDHTRLSCQVRVIDDLHVQVINQAGVKGI
                     DAGTRPVE"
BASE COUNT          351 a          400 c          356 g          399 t
ORIGIN      
        1 tatcacttca gaacatttta tcacttcgcc cggcaaattg tcgcttaacg acaagcgcgg
       61 gatttgagcc gaaatattaa agacaaattt taaataaaga gaaaatcgaa aacgagacga
      121 gcgagacgat tttcgtccgc ctcatttcaa acggctgacg gctttaccca aaatgtcttt
      181 cgccggaatc gcgccgaaac tgcggctgtc tgtgctttcg tcttcgttgt cgccgaccag
      241 aaaatatctg ccttcgggcg agatttccct gattcgtttt atgatttgaa cgcttttttt
      301 gaaaggatgc cgcgccagaa ccacgtcgcc gattcggagc gcggcgtgag gatcgactaa
      361 aaccacgtcg ccgtccttca agcccggcag catcgaatcg ccctggacga gaaaaatctc
      421 tcgaaatccg caaagatacg cgagctgttc ccgccaatcg tccgcgggta attcgttttt
      481 catcaaaata aaaaagatga gcgaagagtt ttttcaactc atccctcatc ccatatcctt
      541 catccttcaa actaagacgc cgtataccac ggaacgtctt tgcctttggt ttcccagaaa
      601 atttcgtgaa cgcgtttgat gttgtccatc aattcctgcg cgtgttcgag gctgatttcc
      661 tgtttgcagg cggagcagag ctttgccgtt ttccagaaaa gctcgtgcag ctcgggatat
      721 ttttccagat gctgcggctt gaaataatcc gtccacagaa ccagcaaatg atgtttcgcc
      781 aaatcggcgc gcgtttcctt aatcgtgata aagcgcgtca aagtgttttg ataagccgcg
      841 tgcgccgtcg cgtcgtcgcc ttcgggacgt ttcaagtcga gaattttttt cgtcagctgc
      901 aaaaccgatt cggcttcgac gcgagtctgc gccgggtcgt agacgccgca cggcgcgtcg
      961 cagtgcgctt cggcgattgc cggattggct gtcagttttt ctttaaattt ttcaaacatt
     1021 ttcccatttc tccttgcttc aataaaaaaa taaaattatt cgaccgggcg cgttccggcg
     1081 tcgatgccct tgacgccggc ttgattgata acctgcacgt ggaggtcgtc aatcacccgc
     1141 acctggcacg acaatctggt gtggtcgctc aaatcggtct tggtcgccag aatggctttt
     1201 tccggctcgc cgatttcgcc cgggtcgccc gccagaactt cgacgcggca cgtcgtgcag
     1261 cgcgcgtttc cgccgcagcg atgtaaaata tcaattccgt tatcttcaag acaaagaacg
     1321 agttttcgtc cttcctcgac ttcaaaagcg agtgttccct gagccgcttc cgcagtaatt
     1381 ttcggcattt ttcgtcaaac tcctgctcat taaatttggt aaaacttcga tactttgaat
     1441 ttggcacaat tcgggcgcga aaagcaagcg cgggagattt cgccccagac ggcaagaagg
     1501 gaaatt
//
LOCUS       DASWNE010000648         2354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_57946, whole genome shotgun sequence.
ACCESSION   DASWNE010000648 DASWNE010000000
VERSION     DASWNE010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57946"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VGB68_12715"
     CDS             <1..193
                     /locus_tag="VGB68_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050148.1"
                     /translation="FDEIKTTGENGIAGERFWIAFRDKEWNETKPPLKNLIEKGYKTG
                     APKLFEAQGLKAFLVEIRK"
     gene            complement(314..1714)
                     /gene="cysS"
                     /locus_tag="VGB68_12720"
     CDS             complement(314..1714)
                     /gene="cysS"
                     /locus_tag="VGB68_12720"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEY0050149.1"
                     /translation="MLRFHNNLSGQIEEFEPISGNEVRMYICGPTVWNFAHIGNFRTF
                     TFGDVLRRYLKYKGYNLTHVMNLTDVDDRIINESKNAGKTIDEFVQPYIEAFWEDADA
                     LGLERPEMAPRATHHIDEMIEIIRKLLDNNHAYEAGGSIYYRISAFPEYGKLSKINFE
                     GNIAGGGGRVDTDKYDKENAQDFALWKFVDADEAAGWDAPFGRGRPGWHIECSAMSIK
                     YLGETFDIHAGGMDLQFPHHENEIAQSEGATGQQFARYWMHGEFLKIDDVTMSKSKGN
                     FFTFRDLREQGYSPLAIRYLLLSIPYRHQLNFTFEGLQGAESTIKRLQDFRRRVKEAR
                     VESGLNAEVERFVLDAVRDFEAAMDDDLNTSQALAAIHNFVREANTALANDALREDDK
                     NLVLGAIEKFDSVLGIFGKDVEEMLDAEIEALIEERQQARRNRDFARSDEIRDLLSEK
                     GIVLEDTKDGVRWKRK"
     gene            complement(1912..>2354)
                     /locus_tag="VGB68_12725"
     CDS             complement(1912..>2354)
                     /locus_tag="VGB68_12725"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY0050150.1"
                     /translation="FAQDTENFACSKVFKTEASEYFENRVERQKFIARCEEKSRLDQI
                     KEFGAPPVKILGGCQWSNDGCPVSLIKPAFPKLAKALKIGGRIPVRIIIDEEGKVIFS
                     RVLGGHPLLKRAARKAACQSIFYPKTACGQKIKQSLIIVYNFVK"
BASE COUNT          578 a          611 c          544 g          621 t
ORIGIN      
        1 tttcgacgaa ataaaaacaa ccggcgaaaa cggcatcgcg ggcgaaagat tctggattgc
       61 gtttcgagat aaggaatgga acgaaacaaa accgccttta aaaaatctga ttgaaaaagg
      121 ctacaaaacc ggcgcgccga aactttttga agcgcagggc ttaaaggctt ttttagtcga
      181 aatccgaaaa taagtgaaaa aagtggtaag tggtaagtgg taagttcgtc gttttgaatt
      241 tcgagctttt atttaaaatt cgattgctcg gcgattgcag cttatcattt accgtttacc
      301 gcttaccgct tttttatttt cgtttccacc tgacgccgtc tttcgtgtct tccagaacga
      361 ttcctttttc gctcagtaaa tcgcggattt cgtcagagcg ggcgaagtct cgattgcggc
      421 gcgcctgctg gcgttcttcg attaaggctt cgatttcggc gtccagcatc tcttcgacgt
      481 ccttgccgaa aattcccaga accgaatcga atttttctat cgcacccagc accagatttt
      541 tatcgtcctc gcgcagagcg tcgttcgcca gcgccgtatt cgcttcgcgg acaaaattgt
      601 gaatcgccgc cagcgcctgc gaggtgttca aatcgtcgtc catcgccgct tcaaaatcgc
      661 gcacggcgtc cagcacaaaa cgctcgacct cggcgttcaa gccgctttcg acgcgcgctt
      721 ctttcacgcg ccggcgaaaa tcctgcaggc gtttaatcgt gctttccgcg ccctgcaaac
      781 cttcaaaggt aaagttcaac tggtgtcgat agggaatcga aagcagcaga taacgaatcg
      841 ccagcggcga atagccctgt tcgcgcaaat cgcggaaagt gaagaagttt cctttcgatt
      901 tcgacatcgt cacgtcgtcg attttcagaa attcgccgtg catccagtat ctggcaaact
      961 gttgtccggt cgcgccttcc gattgagcga tttcgttttc gtgatgcgga aactgcaaat
     1021 ccattccgcc ggcgtgaatg tcgaacgtct cgcccagata ttttatcgac atcgccgagc
     1081 attcgatgtg ccaacccggt cgaccgcgcc cgaaaggcgc gtcccagccc gccgcctcgt
     1141 ccgcatcgac aaatttccag agcgcgaaat cctgcgcgtt ttccttgtcg tatttgtccg
     1201 tatcgacgcg cccgccgccg cccgcgatgt ttccctcgaa atttatcttc gacagcttgc
     1261 cgtattcggg aaaagcggaa atgcggtaat aaatcgagcc gcccgcttcg taggcgtgat
     1321 tgttatccag aagcttccgt ataatttcaa tcatctcgtc gatgtgatgc gtcgcgcgcg
     1381 gagccatctc cggtcgctcc aagccgagcg cgtcggcgtc ttcccagaag gcttcgatat
     1441 agggctggac gaattcgtca atcgtctttc cggcgttttt cgattcgttg ataatgcggt
     1501 cgtcgacgtc cgtcaggttc atcacgtgcg tcagattata gcctttatat ttcagataac
     1561 gccgcaaaac gtcgccgaac gtaaacgtgc gaaaattgcc gatgtgcgcg aaattccaaa
     1621 cggtcggacc gcagatatac attcgcactt cattgccgct gataggctcg aattcctcga
     1681 tttgtcccga taagttgttg tgaaatctaa gcatatattt aaaattcgat gtgaaacaat
     1741 atcataaaag aaaacgacgc gaaaccggat ttttcaaacg gaaaatccga agtcaaaaca
     1801 aattaagcgc gtataaaagg gaatttacgg gcgacaggat tctttcggcg cgatgccgag
     1861 cgcgtgaagc aaatcttttg tcagaagcaa aaacatagac ggatgataac actattttac
     1921 gaaattataa acaataatca gactttgttt gattttttga ccgcaggcgg ttttcggata
     1981 aaaaatggat tgacacgccg ctttccgcgc cgcccgcttt aataacggat gcccgcctaa
     2041 aacgcgcgag aaaatcactt ttccttcctc gtcgataatt attctgaccg gaatccgccc
     2101 gcctattttt aatgctttgg ctaatttcgg aaatgctggt ttaatcaggc tgaccgggca
     2161 accgtcgttt gaccattgac agccgcccaa aatcttcacc ggcggcgcgc cgaattcttt
     2221 aatctgatca agtctgcttt tttcttcaca ccgggcgata aatttttgcc gctcgactcg
     2281 attttcaaaa tattcgctcg cttctgtttt aaatactttc gagcaggcaa aattctccgt
     2341 gtcctgggca aaaa
//
LOCUS       DASWNE010000649         2771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_444212, whole genome shotgun sequence.
ACCESSION   DASWNE010000649 DASWNE010000000
VERSION     DASWNE010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444212"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..1938)
                     /locus_tag="VGB68_12730"
     CDS             complement(28..1938)
                     /locus_tag="VGB68_12730"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEY0050151.1"
                     /translation="MNILSLENVSKNYGIRPLFENVTLGFEDGDKIGIIGANGSGKTT
                     LLRIIAGMEAPDTGRAVRANNKTLAFLSQNPPIDENATVLETIFAASGGVMKLIRDYE
                     IACRDLALQEANQKHLEKVSELQHQLELNGGWEIETNARNVLTRLGIIDTAAQMKTLS
                     GGQRKRVALAHELIVKPDILILDEPTNHLDADTIEWLENYLRRYAGALLLVTHDRYFL
                     DRVTNRIFEIDRAAVQSFAGNYAYYLEKKEEQETLRAVEGHKREQLIKKELAWLRRGA
                     KARTRKSKHRVAAAYDLMAQPKEKAKAEIDIAIGSRRLGSKIIELRNVSKSYGGQKLI
                     EDFTYLLKRDDRIGIIGANGAGKTTLLEIITSRAAPDAGEIEIGQTVAIGYYDQESRA
                     LNDERRVIDYIREVAEYVTTIDGNQITAGQMLEKFLFPPPAQYSVIGNLSGGERRRLY
                     LLRILMSAPNVLLLDEPTNDLDIPTLIALEEYLDDFAGALIVVSHDRYFLDRTIDTVF
                     RFDGAGKIREFPGDYSAYLEIREREQANDSEERAKASAEKTQTSAVEKDKDSAPKKLS
                     FKEKRELETLEAQIPENEKRLAEIESELTRFATDAFKVNQLFTEQQKLNEQLERDMER
                     WAELAERDAEKG"
     gene            2113..>2771
                     /locus_tag="VGB68_12735"
     CDS             2113..>2771
                     /locus_tag="VGB68_12735"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY0050152.1"
                     /translation="MQRCTPNREILSAGYIETFGKREVILRVPFQRVVVDEHKTNNFD
                     FIRLLAASMVVFSHSFYLLATPQRPLNESEPLTFLTGGQMTFGNLGVWIFFAISGYLI
                     TQSLLRSSSYATYFTKRSLRIFPALIVDIVIAAFIWGPIVTTLSLAEYFSSYGTYRYL
                     LNVNLYRIVYELPGVFTDNPIPHFVNGSLWTLPFEFTCYVGIVLLHFLFILRNRHVYA
                     A"
BASE COUNT          547 a          751 c          677 g          796 t
ORIGIN      
        1 tgcttctttt ctccgcgtct cccagcgtca ccctttctcc gcgtctctct ccgccagttc
       61 cgcccagcgt tccatatctc gttcgagttg ttcatttagc ttttgctgct cggtaaaaag
      121 ctgattgact ttgaaagcgt cggtcgcaaa ccgggttaat tcgctttcga tttcagccag
      181 ccgcttttca ttttcgggaa tttgcgcttc cagcgtttca agctcgcgct tttccttaaa
      241 cgagagtttt ttcggcgcgg aatctttgtc tttttcaacg gcgcttgttt gcgttttttc
      301 cgctgaggct ttcgcgcgct cttccgaatc gtttgcctgc tcgcgttctc tgatttccag
      361 atatgccgaa taatcgcccg gaaattcgcg gattttgccc gcgccgtcaa acctgaaaac
      421 cgtatcaatc gtgcggtcga gaaaataacg gtcgtggctg acgacaatca gcgcgcccgc
      481 gaaatcgtcc agatattctt cgagcgcaat cagcgtcggg atgtcgaggt cgttggtcgg
      541 ttcgtccaaa agcagaacgt tcggcgcgct catcaaaatt ctcagcaggt aaagcctgcg
      601 ccgctcgccg cccgaaagat ttccgattac cgaatattgc gccggcggcg gaaacaaaaa
      661 tttttcgagc atctgcccgg cggtgatttg attgccgtca attgtcgtga cgtattcggc
      721 gacctcgcga atgtagtcaa tcacgcgccg ctcgtcgttt aaggcgcggc tttcctggtc
      781 gtaataaccg atggcgaccg tctgcccgat ttcgatttcg cctgcgtccg gcgcggcgcg
      841 gctggtgata atttccagga gggtcgtttt tcccgcgccg tttgcgccga taatgccgat
      901 gcggtcgtcg cgttttaaaa gataggtgaa atcctcgatt aacttttgcc cgccgtagga
      961 tttggaaaca ttgcgcagct cgatgatttt agagccgagc cgcctggagc cgatggcgat
     1021 gtcgatttcg gctttcgctt tttctttcgg ctgagccatc aaatcgtaag cggcggcgac
     1081 gcgatgtttc gactttcgcg tgcgcgcctt tgcgccgcgc ctgagccacg ccagttcttt
     1141 tttaatcagc tgctcgcgct tgtgtccttc gacggcgcgc aaagtttcct gttcttcctt
     1201 tttttccagg taataagcgt agtttccggc aaagctttgc acggcggcgc ggtcgatttc
     1261 aaaaattcgg ttcgtcacgc ggtcgagaaa ataacggtcg tgcgtgacca gcagcagcgc
     1321 gcccgcatat cttcgcagat aattttccag ccattcgatg gtgtcggcgt ccagatgatt
     1381 ggtcggctcg tcgagaatca aaatgtccgg cttgacgata agctcgtgcg ccagcgcgac
     1441 gcgctttctc tgcccgcccg aaagcgtctt catttgcgcg gcggtgtcga taattccgag
     1501 ccgcgttaaa acatttcgcg cgttcgtttc gatttcccag ccgccgttta attccagttg
     1561 atgttgcagt tcggaaactt tttcgagatg tttttgattc gcttcctgaa gcgccaggtc
     1621 gcgacacgcg atttcataat cacgaatcaa tttcatcacg ccgcccgacg ccgcaaaaat
     1681 cgtctccaga acggtcgcgt tttcgtcaat cggcggattc tgcgacaaaa aggcgagcgt
     1741 tttattattt gcccgcacgg cgcgacccgt atcgggcgct tccattccgg cgataattct
     1801 taaaagcgtc gtcttgcccg agccgttcgc gccgataatt ccgattttgt cgccgtcctc
     1861 gaatccgagc gtgacatttt caaaaagcgg tctgatgccg taatttttgc tgacgttttc
     1921 cagcgagaga atattcataa atcagtttca actttactga attatacttt tatcgccaaa
     1981 ttcggaatcc ttttcagaga aatattgcgc gttaatagat ttttgcgccg gaattgcgct
     2041 tcccggcgcg gctaattctt tgtagttttc ttttcatctg ttaaactctc cgaggcaaaa
     2101 aacccactca aattgcagag atgtactccg aaccgggaaa ttctcagcgc aggttatatc
     2161 gaaacgttcg gaaaaagaga ggtgattctt cgcgtgcctt ttcaaagagt tgttgttgat
     2221 gaacacaaaa ccaacaattt cgattttatc cggctgctgg cggcttcgat ggtcgttttc
     2281 tcgcactctt tttatctgct ggcgacgccg cagcgacctt taaacgaaag cgagccgctg
     2341 acgtttctga ccggcgggca gatgactttc ggcaatctcg gcgtctggat atttttcgcg
     2401 ataagcggtt atctgataac gcagagcctt ttgcgcagtt cgagctacgc gacctacttt
     2461 accaaacgaa gcctgcggat ttttccggct ctgatagtcg atatcgtcat cgccgccttt
     2521 atctggggac cgattgtgac gacgctttct ttagccgaat atttttccag ctacggcaca
     2581 taccggtatt tattaaacgt caatctttac cgcatcgttt acgaattgcc cggcgttttc
     2641 accgataatc cgataccgca tttcgtcaac ggctcgctct ggacgctgcc gttcgaattt
     2701 acctgttacg tcggcatcgt cctgctgcac tttctcttta tcttaagaaa ccggcacgtc
     2761 tacgcggctt t
//
LOCUS       DASWNE010000650         4937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_618917, whole genome shotgun sequence.
ACCESSION   DASWNE010000650 DASWNE010000000
VERSION     DASWNE010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618917"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..4641)
                     /gene="rpoB"
                     /locus_tag="VGB68_12740"
     CDS             complement(178..4641)
                     /gene="rpoB"
                     /locus_tag="VGB68_12740"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEY0050153.1"
                     /translation="MIANPLQTNTNGLGRRTSQAPKRVDFSKIYTSARIPNLIEVQRE
                     SYNRFLQMDLLPEERDQIGLQSVFASVFPVSDFRGTATLDFVEYQIGNWQCKCGLLEG
                     LEHLRANCKECGSKIKVDPFSPAEVLCGSCGTFNSVRPKLCSNCGEPVGLKHKHDQQE
                     CQERGMSYSVPLKVKIRLTVYDKDAETGVSTIRDIKEEDVFFGEIPLMTDNGTFIING
                     TERVIVSQLHRSPGVFFKGDRGTYVAKVIPYRGSWIEFEYDQKNILYARLGKRKILAS
                     IFLRALGLWLNPQVEMKNLSDSILEEVIKNSNFSDADILKLFYATDEVRPQKGRLFVK
                     IRPEGESNLVGMRAETEIKGKDEPIVRAGKKIGATALREMRKLGIEEIEVGQTDFEGA
                     FALDDVINTETGEVILEANTELTSAKLQQVIEEGVESFTIFFPERDTIGGVITQTLKK
                     DTITKPVDALLEIYRKMRPGDPPTVPTAYKLLEGMFFDARRFDLSRVGRLKFNIKMGR
                     PERDRINDPLLSASDFTEVVSYLLRMKKDVEHFGQDDIDHLGNRRVRAVGELLENQFR
                     IGLERMERAIKEKMSIQQDMFTTMPRDLVNAKPVTAAVREFFGSSQLSQFMDQTNPLS
                     EITHKRRLSALGPGGLSRERAGFEVRDVHPTHYGRICPIETPEGPNIGLISSLSCFAR
                     INEFGFIESPYRKVEDGRVVEYVKIYNGGDTGFKPNEHVRLEDVEAANEKLKNDQRQA
                     EFVPEPFYLTAWEEDKWIIGQANIELDERGYIVNERNASRKAGEFITATRDEIQYLDV
                     SPKQLVSVAASLIPFLENDDANRALMGSNMQRQSVPLLRAEAPYVGTGMEQVAARDSG
                     AVVVAKRDGIVDYVDSERIIIKADHNVDGTISREVTADIYSLVKFTRSNQNTCINQRP
                     IVAVGERVKKGQVIADGPCTDKGELALGRNVMVAFMPWRGYNFEDAILVSERLVKEDY
                     YTSIHIEELEIEARDTKLGPEEITRDIPNVGEVMLRDLDESGIIRIGAQVKPGSILVG
                     KVTPKGETQLTAEEKLLRAIFGEKAGDVKDASLTAPPGIDGTVVDVQVFTRKGQDKDQ
                     RSLDIEQNEEDDLRRDLEDEIRILQEQRNERIYELFEGRKLVKDLSDAGEVVVKKGEA
                     LTREMLQGVDTKLLRKADVAGGTVDIASEIKDYEQRTERQINILSDIYEEKITKLKQG
                     DELPPGVIKMVKVFIAMKRKLSVGDKMAGRHGNKGVIARILPEEDMPYLPDGTPVDIV
                     LNPLGVPSRMNVGQILETHLGWAARVLGYHFATPVFDGATEAEIKQYIGEANAKFDEL
                     NLPPSVGPSGKTRLYDGLTGEQFEQKVTVGYIYMLKLSHLVDDKIHARSIGPYSLITQ
                     QPLGGKAQFGGQRFGEMEVWALEAYGAAHILQELLTCKSDDVAGRSKIYESIVKGDSD
                     FQAGIPESFNVLVRELQSLCLDVELIMEDEIDKEAINAGVGEVLSVGVGIE"
BASE COUNT         1092 a         1350 c         1240 g         1255 t
ORIGIN      
        1 cgaatctttt cgggcgaggc aaggctgatg cgaatcgctt caaaatttgg cgttaattgt
       61 gttttattat cttgtctaaa aagcattttt tctccataaa gttgactgag tcgtgagttg
      121 actgagtttg tgatttcaac tcagtcaact caattaactt ccagttaact ctgtcaacta
      181 ttcaatacca acaccaaccg ataaaacttc gccgacaccg gcgttaatcg cttctttgtc
      241 aatctcgtct tccataatca gttcgacgtc gagacagagc gattgcagct cgcgcaccag
      301 aacgttgaac gattcgggaa ttccggcttg aaaatcggaa tcgcccttga cgattgactc
      361 gtaaatcttc gagcgtcccg ccacgtcgtc cgacttacag gtcagaagct cctgtagaat
      421 gtgcgccgcg ccgtaagctt ctagcgccca gacttccatc tcgccgaaac gctgtccgcc
      481 gaattgcgct ttaccgccca gaggctgctg cgtaatcaac gagtaaggtc cgattgaacg
      541 agcgtgaatc ttatcgtcga ctaggtgcga aagcttcagc atatagatgt aaccgacggt
      601 taccttctgc tcgaactgtt cgcccgtcaa accgtcgtaa agacgagttt tgcctgacgg
      661 accgaccgac ggcggaagat tcaattcgtc aaatttggca ttggcttcgc cgatatattg
      721 cttgatttca gcttcggtcg cgccgtcaaa aaccggcgtg gcgaaatgat aaccgagtac
      781 gcgcgccgcc cagccgagat gcgtttccag aatctgtccg acgttcatac gcgaaggcac
      841 gccgagcgga ttcaaaacga tgtcgaccgg cgttccgtcc ggcaagtaag gcatatcttc
      901 ttccggcaga atgcgggcga taacgccttt gttgccgtga cgaccagcca ttttatcgcc
      961 gacgctcaat ttgcgtttca tcgcgataaa gactttgacc attttgatga cgcccggagg
     1021 aagctcgtcg ccctgcttca gcttggtgat tttttcctcg taaatgtcgc tcaaaatgtt
     1081 gatttgacgc tcggttctct gctcgtaatc cttgatttcc gaagcaatgt caaccgttcc
     1141 gcccgccacg tccgccttgc gaagcagctt cgtatcgacg ccttgcagca tttcgcgcgt
     1201 cagcgcttcg ccttttttga caacgacttc accggcgtcg ctcaaatctt tgacgagctt
     1261 gcgaccttcg aaaagctcgt aaatacgctc gttgcgctgt tcctgcaaga tgcggatttc
     1321 gtcttcgagg tcgcggcgca aatcgtcttc ttcgttttgc tcgatgtcca atgagcgctg
     1381 gtctttgtcc tgacctttgc gcgtgaaaac ctgaacatcg acgaccgtgc cgtcgattcc
     1441 cggaggcgcg gtcagagaag cgtctttcac gtcgccggct ttttcgccga agatggcgcg
     1501 cagaagtttt tcttccgccg tcagctgcgt ttcgcctttc ggcgtcactt tgccgaccag
     1561 aatcgagccg ggcttgacct gcgcgccgat gcggataatt cccgattcgt ccaaatcgcg
     1621 gagcataact tcgccgacgt tgggaatatc gcgcgtgatt tcttcgggtc cgagcttggt
     1681 gtcgcgcgct tcgatttcca gctcctcgat gtgaatcgaa gtgtaataat cttctttcac
     1741 aaggcgctcg gaaaccagaa tcgcgtcctc gaaattgtag ccgcgccacg gcataaacgc
     1801 gaccatcacg tttctgccca aagcgagctc gcctttatcg gtgcagggtc cgtcggcgat
     1861 aacctgacct tttttcacgc gctcgccgac cgcgacaatc ggacgttgat tgatacaagt
     1921 gttttgattc gagcgagtaa atttgacgag cgagtaaatg tccgccgtca cctcgcgcga
     1981 aatcgtgccg tccacgttat ggtcggcttt gatgataatg cgctccgaat cgacgtaatc
     2041 gacgatgccg tcgcgctttg ccacgacgac cgcgcccgaa tcgcgcgccg cgacctgttc
     2101 cataccggtt ccgacatagg gagcttcggc gcggagcagc ggcacggact ggcgctgcat
     2161 gttcgagccc atcaaagcgc ggttcgcgtc gtcgttttcg agaaacggaa tcagggatgc
     2221 cgcaaccgaa acaagctgtt tcggcgaaac gtccagatat tgaatctcgt cgcgcgttgc
     2281 cgtgataaat tcgccggctt tacgcgaagc gttgcgctcg ttgacgatat aacctctttc
     2341 atcgagctcg atatttgcct gaccgataat ccatttgtct tcttcccagg cggtcaggta
     2401 aaacggctcg ggcacgaatt cagcctgacg ctggtcgttt ttgagctttt cgtttgccgc
     2461 ttcgacgtct tccagacgaa cgtgttcatt cggcttaaat ccggtgtcgc cgccgttgta
     2521 gattttgacg tattcgacga cccgcccgtc ttcgactttg cgatacggcg attcgataaa
     2581 accgaactcg tttatccgcg caaagcacga caaactcgaa atcaagccga tgttcggacc
     2641 ttcaggcgtt tcaatcggac agatgcgacc gtaatgggtc gggtgcacgt cgcggacttc
     2701 gaatccggcg cgctcgcggc tcaagccgcc cggtccgagc gcggacaaac gacgcttgtg
     2761 cgtgatttcc gaaagcggat tcgtctggtc cataaactgc gacagctgcg acgaaccgaa
     2821 aaactcacga accgccgccg tgacgggctt cgcgttgacg aggtcgcgcg gcatcgtcgt
     2881 aaacatgtcc tgctggatgg acattttttc cttgatggcg cgttccattc tttccagacc
     2941 gatgcgaaat tgattttcca gcagctcgcc gacggcgcgg acgcggcgat tgcccaggtg
     3001 gtcaatatcg tcctgcccga aatgctcgac gtcttttttc atccttaaca gataagaaac
     3061 gacttccgta aaatccgacg ccgacaaaag cgggtcgtta atgcggtcgc gttccggtct
     3121 gcccatcttg atattgaatt tcaagcgtcc gacgcgcgat aaatcaaagc gccgcgcatc
     3181 gaaaaacatt ccttcgagca atttataagc ggtcggaacg gtcggcgggt cgcccggacg
     3241 cattttgcga taaatttcca aaagcgcgtc gaccggcttg gtaatcgtgt cttttttcaa
     3301 agtctgtgta atgacgccgc cgatggtgtc gcgttccgga aagaaaatcg taaagctttc
     3361 cacgccttct tcgattacct gctgcaactt ggcggaagtc agctccgtgt tggcttcgag
     3421 aataacttcg cccgtttccg tattgatgac gtcgtctaaa gcgaacgcgc cttcaaaatc
     3481 ggtctgcccg acctcgattt cttcgatgcc cagcttgcgc atttcgcgca gagcggtagc
     3541 gccgattttc tttccggcgc ggacgatcgg ctcgtcttta cctttgattt ccgtctccgc
     3601 gcgcatcccg accagattgc tttcgccttc cggtctgatt ttgacgaaca ggcgtccctt
     3661 ttgcgggcgc acttcgtccg tcgcataaaa taatttcaaa atatcggcgt cggaaaaatt
     3721 cgagtttttg atgacttctt ccagaatcga atccgaaaga tttttcatct cgacctgcgg
     3781 gttgagccac aagcccagcg cgcgcaggaa aatcgaagcc agaattttgc gcttgccgag
     3841 gcgcgcgtaa agaatatttt tctggtcgta ttcaaactcg atccacgaac cgcgatacgg
     3901 aatgactttc gccacgtaag ttccgcggtc gcctttgaaa aacacgccgg gcgagcggtg
     3961 gagctgcgaa acgataacgc gctccgtgcc gttgatgata aaagttccgt tgtcggtcat
     4021 cagcggaatc tcgccgaaga aaacgtcttc ttccttaatg tcgcgaatcg tcgaaacgcc
     4081 ggtttcggcg tccttgtcat aaaccgtgag acggattttg acttttagcg gaacgctgta
     4141 gctcatcccg cgttcctgac attcctgctg gtcgtgcttg tgtttcaaac cgaccggctc
     4201 gccgcagttt gagcagagct tcgggcgcac cgagttaaaa gtcccgcagc ttccgcagag
     4261 aacttccgcc ggactgaacg ggtcgacctt gattttcgag ccgcattcct tacaattggc
     4321 gcgcaaatgt tcgagacctt cgagaagtcc gcatttgcac tgccagttgc cgatttgata
     4381 ctcgacgaaa tccagcgtcg ccgtgccgcg aaaatccgaa accggaaaaa ccgaggcgaa
     4441 gaccgattgc agcccaatct ggtcgcgctc ttcgggcagt aaatccatct gcaaaaaacg
     4501 attgtatgat tcacgctgaa cctcgattag attcgggatg cgtgccgatg tataaatttt
     4561 tgaaaaatcg acccgcttag gcgcctggct cgtgcggcga cctaagccgt ttgtatttgt
     4621 ttggagcgga ttggcaatca tattttttaa aattctgaag aaaatttttg ttgctttgta
     4681 cacgttttgc tggtagtatt cgtaatcgtt gcaaacgaat cctcagcaaa caacttttta
     4741 acaaagacgg caaatgcggt cgagggcaaa ctttgcccag accgcttaaa attagcagtg
     4801 actgctacga aataaatttt ctgagtgtgc cgaaaaagga cacctattct cccgttttat
     4861 gtcggaaaaa accaaattta aagctcaaaa tctgctttga gctttaaaaa gaacgaaatc
     4921 ggcaagcgac gagtttt
//
LOCUS       DASWNE010000651         1690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_126871, whole genome shotgun sequence.
ACCESSION   DASWNE010000651 DASWNE010000000
VERSION     DASWNE010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126871"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /gene="sat"
                     /locus_tag="VGB68_12745"
     CDS             complement(<1..865)
                     /gene="sat"
                     /locus_tag="VGB68_12745"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HEY0050154.1"
                     /translation="MTPTIIKETHLIAPYGNTLKNLLVSPEETADFKKYANALPSVQL
                     SARAICDLEMLAVGAFSPLESFMNEADYRRVVEEMRLTSNHVFPIPVALPLDAESGIR
                     ENREIALRDAKNNLLATMKIEEIYEWNRAEFAREVLGTEDARHPLAAEMSGWGRLNVS
                     GSLRVVELPRYYDFPELRLSPAAVRARLESLGNECVVAFQTRNPLHRAHEELTKRALE
                     KTGGTLLLHPVVGLTRAGDIDYYSRVQTYKILTEKYYDKKSVLLALLPLAMRFAGPRE
                     ALWHAVIRRNYG"
     gene            complement(983..>1690)
                     /locus_tag="VGB68_12750"
     CDS             complement(983..>1690)
                     /locus_tag="VGB68_12750"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY0050155.1"
                     /translation="RPRDLDILLVGPSGEAFILMSDVAGSTDTADSNNVTITLADGSP
                     ALPSDAVGTTMLVSGTFRPTNNGNGEGTLPAPAPAGPYNNPPNAPGGTACTGCTSTFA
                     SVYAQPGVLANGVWSLYVDDDGTGGAVGANTMAGGWTLSVTTDGIPTTAAGANVSGRV
                     VNSEGRGIAGVKIGMIEPNGQTRYALANPFGYFNFANVPAGETYVFSVKAKGYQPTSI
                     TRAVAEDVADLEIVLHE"
BASE COUNT          342 a          486 c          485 g          377 t
ORIGIN      
        1 ctccatagtt gcggcgaatg acggcgtgcc acaaagcctc gcgcggtccg gcaaagcgca
       61 tcgccagcgg cagcagcgcc agcagaacgc tctttttgtc gtaatatttt tcggtcagaa
      121 ttttatacgt ctgcacgcgg ctgtaataat cgatgtcgcc cgccctggtc aaaccgacga
      181 ccggatgcaa aagcaaggtt ccgcccgttt tttctagcgc gcgtttggtt aattcttcgt
      241 gcgcgcggtg aagcggattg cgcgtttgaa atgcgacgac gcattcgttg ccgagcgatt
      301 cgagccgcgc gcgaacggcg gctggcgaca ggcgcaattc gggaaaatcg taataacgcg
      361 gaagctcgac cacgcgcagg ctgcccgaaa cgttcagcct gccccatccg ctcatttccg
      421 ccgccagcgg atggcgcgcg tcttcggttc ctaaaacttc gcgggcgaat tcggcgcgat
      481 tccattcgta aatttcctcg attttcatcg tcgccagcag attgtttttc gcgtctcgca
      541 gggcgatttc ccgattttca cgaattccgc tttcggcatc gagcggcaaa gcgacgggaa
      601 tcggaaaaac gtgattgctc gtcagacgca tttcttcgac cacacgtcga taatcagcct
      661 cgttcataaa gctttcgagc ggagaaaaag cgccgaccgc cagcatttcc aaatcgcaaa
      721 tcgcccgcgc cgaaagctgc acggacggca gagcgttggc gtattttttg aaatcggcgg
      781 tttcttccgg cgaaacgaga agatttttca gcgtgtttcc gtagggcgcg atgagatgcg
      841 tttcttttat tatcgtcggt gtcacaaaat attttccggg ctgatgatga ttttacaaaa
      901 gaagcgtgcc cgaccgaaat agtcgaacac gctctttaca gttttcagtt cgggacaggc
      961 atttgcctgc ctgaagcggt aattattcgt gtaggacgat ttccaaatca gccacatctt
     1021 ccgccaccgc tctggtgatg ctggtcggct gatagccttt cgccttgacg ctgaaaacat
     1081 aggtttcgcc agccggaacg tttgcgaaat tgaaatagcc gaacgggttg gctaatgcat
     1141 aacgcgtctg tccgttcggt tcgatcattc cgattttcac gccggcaatt ccgcgtcctt
     1201 cagaattcac cacgcgtccg ctgacgttcg cgcccgcggc ggtcgtggga atgccgtcgg
     1261 tcgtcacgct cagcgtccag ccgcccgcca tcgtgttggc gccgaccgcg ccgcccgtgc
     1321 cgtcgtcgtc cacgtagaga ctccagacgc cgtttgccag aacgcccggt tgagcgtaaa
     1381 cgctggcgaa agtcgaagtg cagccggtgc aggcggttcc gcccggagcg ttcggcggat
     1441 tgttatacgg accagccgga gcgggagcgg gcagcgtgcc ttcgccgttt ccgttattgg
     1501 tcgggcggaa agtgcctgag accagcatcg tcgtgccgac cgcatcggaa ggcagagcgg
     1561 gcgagccgtc cgccagcgtg atagtcacgt tatttgaatc cgcggtgtcg gtcgagcccg
     1621 caacgtcgga cattaaaata aacgcctcgc cgctcggacc gaccagaaga atatcgaggt
     1681 cgcgcgggcg
//
LOCUS       DASWNE010000652         2794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_270803, whole genome shotgun sequence.
ACCESSION   DASWNE010000652 DASWNE010000000
VERSION     DASWNE010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270803"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            399..>2794
                     /locus_tag="VGB68_12755"
     CDS             399..>2794
                     /locus_tag="VGB68_12755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050156.1"
                     /translation="MRYDEFTLTPSANLPTFAAGRWVDPNNAYRGNLTSIRSYTDPAD
                     NQYIETHNYYDKYGNLRKSVDGRGYESQTEYSADYACAYPTKSISPVPDPDNVNGSSA
                     ALETIISYDLNTGLPRYTIDPNNQITEIQYNDSLLRPTKAIAPNGQQTVTQCGAATSA
                     ATRFVKNKTQIDAEKWNEVDTYFDGLGRTIKTQSVDSDGDVFVITNYDEMGRVEEVSN
                     PFRNVSNPSCATNLECTSTTYDDFGRVWKVRTPDGAIVETTYALATAGAQIGTVTTVK
                     DQAAKERRSITNGLGQLTRVDEPDEAGQLGAIQNPNQPTVYAYDTLNNLTSVTQGAQT
                     RTFVYDALARLKSATNPESGTINYSYDAGGNLLAKTDARGVLTSYAYDPLNRILARAY
                     GNEPGGQTPTPAVAYFYDNIQNAKGKLVKVSNSHSTTEYTAFDVSGRPAAHKQTTDGQ
                     SYTTAYEYNLAGALLAETYPTGRVVKNILDADGELSTVQSKKNQNAGFWTYAAHFTYT
                     AAGAVSSMQLGNGHWKSTEFNSRLQPVRIALGATQNGNDLLKLDFTYNTPNAADNNGN
                     VRAQSITVPAAGGNPGFTAIQSYTYDSLNRIKQASETVGGVQTWKQTFKYDRYGNRNF
                     DTTGNQTTTLESGSLPKVVNPEIQASNNRFKTDQDSDGTADYLYDASGNTTRDALNRS
                     FIYDAENKQIEVLENNVSLGKYFYDGDGKRVKKVTNNETVIFVYDAIGKLIAEYSTQL
                     NPTPQVAYLTNDHLGSPRINTDALGSVISRHDYQPFGEEIERASYGTDDIRKQFTGYE
                     "
BASE COUNT          785 a          778 c          726 g          505 t
ORIGIN      
        1 gccggcgatg ccggcaactg gtatatccgc aacagctcga ccgggcaaac cagaatcgag
       61 cactggggaa ttacgggcga tattcccgtg ccggcttttt atcgaagata aaacctgaca
      121 attaaaaaca agaattaaat cgaggattaa atctgaaaac cagcgagcaa tacgatttta
      181 tattagtaga tgcgggtagt tccgcaagcc cggcgcaaac cgaaaacgcc gcgcagctgg
      241 cggctcgttt taacagtatt gtcccggtta aacggtcgga gcttgactac ctgtacaaca
      301 caaattataa ttcgccggat acgagtcttt tagggattgt catcgagcag cgagtaatca
      361 atccggcgaa caataatcaa atcgtctccc gagcccagat gaggtatgac gaattcacat
      421 tgacacccag cgccaatctg cctacgttcg ccgccggcag atgggtagac cccaataacg
      481 cttaccgcgg caacctgaca agcatccgca gctacaccga ccccgccgat aatcaataca
      541 tcgaaactca caattactac gataaatacg gcaatctgcg caaatcggtc gatggacgcg
      601 gctatgaaag ccagaccgaa tacagcgccg attatgcctg cgcgtatccg acaaaaagca
      661 tttccccggt tcccgacccg gacaatgtga acggttcgag cgcggcgctg gaaacgatta
      721 tttcttacga tttaaacacg ggattgccgc gctacacgat tgacccgaac aatcaaatta
      781 ccgagataca atataacgat tcgcttttgc gcccgaccaa agcaatcgcg ccgaacgggc
      841 agcagaccgt cacccaatgc ggcgcggcga cgagcgccgc cacgcgcttc gtcaaaaaca
      901 aaacgcagat tgacgccgaa aaatggaacg aagtcgacac ttactttgac ggtctgggca
      961 gaacgattaa aacgcagtcg gtcgattcgg acggcgacgt tttcgtgatt acgaattacg
     1021 atgagatggg acgagtggaa gaagtttcca acccgtttcg caacgtctcg aacccgagct
     1081 gcgcgacgaa tctggaatgc acttcgacga cttacgatga tttcgggcgg gtgtggaaag
     1141 tgaggacgcc cgacggcgca atcgtcgaaa cgacttacgc gctggcgacg gcgggcgcgc
     1201 aaatcggaac ggtgacgacc gttaaagacc aggcggcgaa ggaaaggcgc tcgattacca
     1261 acgggctcgg gcaattaacc cgcgtcgacg agccggacga agccggacag ctcggcgcga
     1321 ttcagaaccc gaaccagccg accgtttacg cttacgacac gctgaataat ttaaccagcg
     1381 tcacgcaggg cgcgcagacc aggacgtttg tttacgacgc gctggcgaga ttgaaatcgg
     1441 cgacgaatcc cgaatcggga accatcaatt attcgtatga cgccggcgga aatctgctgg
     1501 cgaaaacgga cgcgcgcggc gttttgacga gctatgcgta cgacccgctc aatcgcatcc
     1561 tcgccagagc ttacggcaac gagccgggcg gacagacgcc gacgccggcg gtcgcgtatt
     1621 tttacgataa tatccagaac gcgaaaggca agctcgtaaa agtctccaat tcacattcga
     1681 cgaccgaata caccgccttc gacgtttcgg gcagaccggc ggcgcacaag cagacgaccg
     1741 acgggcagag ctacacgacc gcttacgaat acaatctggc gggcgcgctg ctcgcggaga
     1801 cttacccgac gggcagagtc gtgaaaaaca ttctggacgc ggacggcgaa ttgtcgacgg
     1861 tgcaaagcaa aaaaaatcag aacgcgggct tttggactta cgccgcgcat ttcacataca
     1921 cggcagcggg cgcggtttcc tcgatgcagc tcggcaacgg gcattggaaa tcgaccgagt
     1981 tcaattccag attacagccc gtgcgaatcg ctctcggcgc gacacagaac ggaaacgatt
     2041 tattaaagct ggattttaca tacaacacgc cgaacgccgc cgacaataac ggcaacgtcc
     2101 gcgcgcagag catcaccgtt ccggctgcgg gcggcaatcc gggctttacg gcgattcagt
     2161 cttacacata cgacagcttg aaccgcatca aacaagcctc ggaaaccgtc ggcggcgttc
     2221 aaacgtggaa acaaaccttc aaatacgacc gctacgggaa tcgcaatttt gacacgaccg
     2281 gcaaccagac gacgacttta gaatcgggta gtctgccgaa agtcgtcaat cccgaaattc
     2341 aagcctcgaa caatcgtttc aaaaccgacc aggactcgga cggcacagcc gattacctgt
     2401 acgacgcatc aggcaataca acgcgtgacg ctctgaatcg aagcttcatc tacgacgccg
     2461 agaacaagca aatcgaagtg ctggaaaaca acgtttccct cggcaaatat ttctacgacg
     2521 gcgacggcaa gcgcgtcaag aaagtaacga ataatgaaac cgtcattttc gtttacgatg
     2581 ccatcggaaa actgattgcc gaatattcga cacagcttaa tccgacgccg caagtcgcct
     2641 atcttacaaa tgaccattta ggcagcccgc gcattaacac ggacgctctc ggcagcgtga
     2701 tttcccgcca cgattaccag ccgttcggcg aagagataga aagagccagc tacggcacgg
     2761 atgatataag aaaacagttt acgggttatg aaag
//
LOCUS       DASWNE010000653         2063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_270826, whole genome shotgun sequence.
ACCESSION   DASWNE010000653 DASWNE010000000
VERSION     DASWNE010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270826"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..443)
                     /locus_tag="VGB68_12760"
     CDS             complement(57..443)
                     /locus_tag="VGB68_12760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0050157.1"
                     /translation="MTNLNIAERRNNDITILDLDGKIKLGEGCAELHKALRLLIEKGE
                     RKVLLNLEKVSYIDSSGLGELVSGYAAFKRNDAEMKILNLSQSVHQLMVLTKLLTVFQ
                     IYDNETTAVENFDAPAVNTAHVSRLA"
     gene            complement(539..1021)
                     /locus_tag="VGB68_12765"
     CDS             complement(539..1021)
                     /locus_tag="VGB68_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050158.1"
                     /translation="MTNLPVNENLLDAEQRLKKATSDNIVALCEKYLQSLNAYREELY
                     KFQGAPEINRQQASASAREMVEQKRNAIRTELEIVTRKRNETESLLQSFISINGYEAV
                     NTFNRLEYKGFTSWELRANEVRPKNDDNNERMTVSEAVDTASLLRREAFITYQTTFLR
                     "
     gene            complement(1079..1501)
                     /locus_tag="VGB68_12770"
     CDS             complement(1079..1501)
                     /locus_tag="VGB68_12770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY0050159.1"
                     /translation="MRNLNITERRSGKVMIVDLEGNIRLGEGSMEFRQFIRRTVQNGE
                     KNILLNLAGITYIDSSGLGEIVAAYTSVQKIDGEMKLLHLTRRVNELMMITKLLTVFE
                     VFDNEAQALGSFQKEPENTGNKPLSAAAGKPNPALINL"
     gene            complement(1855..>2063)
                     /locus_tag="VGB68_12775"
     CDS             complement(1855..>2063)
                     /locus_tag="VGB68_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050160.1"
                     /translation="ENQTVWTFTADIPKLISEGWSPGAITVLIDKTDGHVLTEKQEAD
                     FHKNWENTRRRAGFVKPKRNAAGE"
BASE COUNT          435 a          521 c          475 g          632 t
ORIGIN      
        1 agccatcgga atttttctcg attttcttta tttttgtgca aagcaggtct ggtgctttac
       61 gctaaacgac tgacgtgagc cgtattaacc gccggcgcat cgaaattttc gactgccgtc
      121 gtttcgttgt cgtaaatttg aaaaaccgtc agaagtttgg tcaacaccat caactgatga
      181 acgctttgcg ataaattgag aattttcatc tccgcgtcgt ttcttttaaa ggcggcgtaa
      241 ccgctgacca gctcgccgag cccgctcgaa tctatatagg aaaccttttc cagattcagc
      301 agaactttcc tttcgccctt ttctatcaaa aggcggagag ccttgtgcag ctcggcgcag
      361 ccctcgccga gctttatttt tccgtctaaa tccagaatgg taatgtcgtt gttgcggcgt
      421 tcggcaatat taagatttgt cataaaattt tcctttttta ttttcaggca gtgtcgataa
      481 attaaacggt ttttatatta tcgaagtttc aaattaaacc gcgctcaatc ttttttcgct
      541 accgcagaaa tgtcgtctga taggtaatga aagcttcgcg ccggagcagg ctcgccgtat
      601 cgacggcttc gctgacggtc attcgctcgt tgttatcgtc gtttttcggg cgaacttcgt
      661 ttgccctcag ctcccagctg gtaaatccct tgtattcgag ccggttaaat gtatttaccg
      721 cctcgtagcc gtttatcgag ataaatgatt gaagcaggga ttcggtttcg ttacgtttgc
      781 gcgtgacgat ttccagctcc gtgcgaatcg cgtttctctt ttgctcgacc atctcgcgcg
      841 ccgaagcgct cgcctgctgc cgattgattt ccggcgcgcc ctgaaattta taaagctctt
      901 cgcggtaggc gttcagcgat tgaagatatt tttcacaaag cgcgacgata ttatcgctcg
      961 tcgctttttt cagcctttgt tcggcgtcga gcaggttttc attgaccggt aaattcgtca
     1021 ttaacataaa tcgccccgaa gctcgccgtt ttgacgacgg cttcaaatcc tttcggtttt
     1081 ataaatttat taatgccgga ttcggctttc cggcagcggc ggacaatggt ttattcccgg
     1141 tattttcggg ttctttctga aaactcccga gcgcttgcgc ctcgttatca aaaacttcga
     1201 aaaccgtcag cagttttgta atcatcatca gttcgttaac gcgccgggtc agatgcagga
     1261 gcttcatctc gccgtcgatt ttctgcaccg acgtgtaggc ggcgactatc tcgcccaaac
     1321 cgctcgaatc gatgtatgtg atgcccgcca ggttgagcag aatgttcttt tctccgttct
     1381 gcaccgtccg gcggataaat tggcgaaatt ccatactgcc ttcgccgagc ctgatgttgc
     1441 cttccaggtc tacaatcatt actttgccgc tgcggcgttc ggtaatatta agatttctca
     1501 tttttatttt cctttgattt ttattcgaca agctcaaaaa tcgagcttgc tgcataaacg
     1561 ccgcgccgcg ctgtattgcc cgacgcgccc gtttaaaatg ccgaaagaat tactcgatgc
     1621 ttatcgctca aatggtaagg cgattcgcct ggcggttttc ctttcggcat tttaagcggg
     1681 tgagctgccc tttctcactt tttaccggaa atttaagttt aaacaatcag acgcggcgcg
     1741 tctgtgcggc gccgcgctta taaatactat attttacagc gtttatttat cggcggcggg
     1801 tttcctgcga aacaataaaa acgaggcggg aaccttgccg gacggcagat tgctttactc
     1861 tccggcggca ttccgtttcg gttttacaaa gcccgcgcgg cgacgcgtgt tttcccagtt
     1921 tttatggaaa tcggcttcct gtttttccgt caaaacgtgc ccgtccgttt tatcaatcag
     1981 gacggtgatg gcgccgggcg accagccttc gctgatcagc ttcggaatat cggcggtaaa
     2041 agtccaaacc gtttgatttt cac
//
LOCUS       DASWNE010000654         1641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_514652, whole genome shotgun sequence.
ACCESSION   DASWNE010000654 DASWNE010000000
VERSION     DASWNE010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514652"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VGB68_12780"
     CDS             <1..402
                     /locus_tag="VGB68_12780"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY0050161.1"
                     /translation="VKVDPDTGFVRVTRAIEVSACGRIMNPKTSHSQEIGGVVWGIGM
                     ALEEATEVDHRFGRIMNPNLQHYHVPVNADIQEIETIFVEEDDRIVNPLGVKGMGELG
                     MVGIPAAIANAVFHATGKRIRELPITPDKLL"
     gene            479..751
                     /locus_tag="VGB68_12785"
     CDS             479..751
                     /locus_tag="VGB68_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050162.1"
                     /translation="MNKNTTKSEYAGDYAASNDFYRKSRQIGETLDGFVAARRRQIER
                     ALEFAARADELRRRIRHTLKLPDRIFRDNYTARLALYAGRQDADRQ"
     gene            complement(976..>1641)
                     /locus_tag="VGB68_12790"
     CDS             complement(976..>1641)
                     /locus_tag="VGB68_12790"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0050163.1"
                     /translation="KADLAVWRPSEGVWYVLGSTRGFFAAQFGANGDVPVAGDYDADG
                     KTDFAVFRPSNGSWYIQQSTLGFRATQFGISTDKPMIGDFEGDGKTDLAVWRASNGTW
                     YILQSSSSLLLGAKFGINTDLPLTGDFDGDNKTDLAVYRPNEGNWYIMNSLNNSLGVY
                     RFGVASDLPVPADYDGDGKTDVAVFRASENAWYRLNSTNGAFVAKNFGQNGDVPSPSS
                     MQP"
BASE COUNT          416 a          432 c          423 g          370 t
ORIGIN      
        1 gtgaaggtcg atccggacac cggatttgtc cgcgtcacgc gcgcgataga agtttcagcc
       61 tgcgggcgga ttatgaaccc gaaaacttcg cacagccagg aaatcggcgg cgtcgtctgg
      121 ggcatcggaa tggcgctcga agaggctacc gaagtcgacc atcgtttcgg gcgaataatg
      181 aacccgaatt tgcagcatta tcacgttccg gtcaacgcgg acattcagga aatcgaaacg
      241 attttcgtcg aagaggacga ccgaatcgtc aacccgctcg gcgtcaaagg aatgggcgag
      301 ctcggcatgg tcggaatacc ggcggcgatt gccaacgcgg tctttcatgc aaccggcaag
      361 cgcatcaggg aacttcccat cacgccggac aaactcttat aggagattct tttaaagggt
      421 tataagcgta tcgtcgggat tttgaaacct cgacgatacg cttttctctt aaattaccgt
      481 gaataaaaat acgacaaaat ctgaatatgc gggcgattac gccgcgtcaa acgattttta
      541 ccggaaaagc cgtcagattg gagaaacgct cgacggcttt gtcgccgccc gtcgccgtca
      601 aatcgaacgc gcgctcgaat tcgccgcgcg agccgacgaa cttcgccggc gcattcgtca
      661 cacgctcaaa ctgcccgacc ggatttttcg cgataattat acggcgcgcc tcgccttata
      721 cgccggtcgg caagatgccg accggcagta gaaactctgc gtcaacgagc cgttcgttcg
      781 ataaatttaa ttgccggttt cgcccgcgaa tatttttata ttttcataat aaagaaaacc
      841 gtcgagcggg aagtcgaatt ttttaaagcg attggaaaat agaagatttc agcgcttcga
      901 ttgaagtagt ggagttatca tcatagttgt aaacgactct taaatcggaa aaatcacgcc
      961 ttccatttta aagttttaag gctgcattga agaaggcgaa ggaacatcgc cgttttgacc
     1021 gaagtttttc gctacaaatg cgccgttcgt gctgttcaga cgataccagg cattttcaga
     1081 ggcgcgaaac accgccacat ccgttttgcc gtcgccgtcg taatcagccg gaaccggtaa
     1141 atcgctcgcc acgccgaaac gataaacgcc cagtgaattg ttcaggctgt tcataatata
     1201 ccagttgcct tcattcgggc gataaaccgc caaatccgtt ttattgtcgc cgtcgaaatc
     1261 tccggtcagc ggcaaatccg tattgatgcc gaatttagcg cccagcaaaa gactcgaaga
     1321 gctttgcaga atataccagg ttccgtttga cgctcgccag acagccaaat ccgttttgcc
     1381 gtcgccttcg aaatcgccga tcatcggttt atcggtcgag atgccgaatt gcgtggcgcg
     1441 aaatcctaaa gtgctctgct ggatatacca cgagccgttt gaaggacgga aaacggcgaa
     1501 atcggttttg ccgtcggcat cgtaatcgcc ggcgaccggg acatcgccat tggcgccgaa
     1561 ttgagcggcg aaaaatccgc gcgtgctgcc cagaacatac cagacgcctt cgctcggacg
     1621 ccagacggcg aggtcggctt t
//
LOCUS       DASWNE010000655         1819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_373780, whole genome shotgun sequence.
ACCESSION   DASWNE010000655 DASWNE010000000
VERSION     DASWNE010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373780"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..477
                     /locus_tag="VGB68_12795"
     CDS             196..477
                     /locus_tag="VGB68_12795"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HEY0050164.1"
                     /translation="MQLARVIGTVVSTIKNESLNGRKFLIVQTLDADLKPKGKPLVAL
                     DSVGAGVGELVFWCRGKEASFPFRRDSTPTDCTIVGIVDSDEHVYTGDN"
     gene            536..829
                     /locus_tag="VGB68_12800"
     CDS             536..829
                     /locus_tag="VGB68_12800"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HEY0050165.1"
                     /translation="MLLAKVIGNVVATQKNQRYEGTRVMLCRQITPEGEEMENTLIAL
                     DSVSAGEGDIVIVVQEGWSASTAATGKAGAAIDSAIIGVVDYIDLLPDEKKLQ"
     gene            829..1176
                     /locus_tag="VGB68_12805"
     CDS             829..1176
                     /locus_tag="VGB68_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050166.1"
                     /translation="MTNENTQKLAERIALLLQETEQPKDDFSALQTTLEKINRRLDKI
                     ESQFAAQNLNKTQHSALAAAHLKPHPSQEKFAIDEAVVNEILEHFEKEKACTFEPNGK
                     PCDHCAMCSSRGF"
     gene            complement(1178..>1819)
                     /locus_tag="VGB68_12810"
     CDS             complement(1178..>1819)
                     /locus_tag="VGB68_12810"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY0050167.1"
                     /translation="RKEIKTMRKTILLTIALLVAFGASGCSYNDLTAQQQGVRGKWAN
                     VESNLQRRADLIPNLVKAAQMSAIQEQEVFGQIADARSRLLNAVQAPPAGADGDKTPE
                     QKQAVIDANNSFGGTIGRLLSLQESYPVLRSNEAFMKVQDELSGTENRINTARLDYND
                     AVTKYNTARNSFPAVITASMFGFKEEPFFKADEGSRQAPNLGDPNSIRRDANK"
BASE COUNT          466 a          465 c          463 g          425 t
ORIGIN      
        1 aaaggcggcg aaaatctggc gcaggcgcat cgttatctgg gcggcatcta tatacagaaa
       61 aaacgcaacg ccgaagccat cgccgagctg cagaaatttc tggatttgtc gccgaaagcg
      121 ccggacgccg ataaaattaa agcgataatc gaagatttga aaaagaaaag ctaagaaagc
      181 gccggattca ggaaaatgca gcttgctcgc gtcatcggaa cagtcgtttc caccattaaa
      241 aacgaatcgc ttaacgggcg aaagtttttg attgtgcaga cgctcgacgc cgatttaaaa
      301 ccgaaaggca agccgctggt cgcgctcgat tcggtcggcg cgggcgtcgg cgaactggtt
      361 ttctggtgtc gcggaaagga agcctcgttt cctttccggc gcgacagcac gccgaccgac
      421 tgcacgattg tcggcattgt cgattcggac gaacacgtct acacaggtga taactgataa
      481 ctgatagttt ttctttcact atcagttatc ggttatcagt tatcagttaa aaaaaatgct
      541 tttagccaaa gtcatcggaa acgtcgtcgc cacgcagaaa aatcagcgtt acgaaggcac
      601 gcgcgtgatg ctctgtcgtc aaatcacgcc tgaaggcgaa gaaatggaaa acacgcttat
      661 cgcgctcgat tcggtttcgg cgggcgaggg cgatattgtt atcgtcgtgc aggaaggctg
      721 gagcgcttcg accgccgcga ccggaaaagc gggcgcggcg attgattcgg caatcatcgg
      781 cgtggtcgat tacatagatt tattgccgga cgagaagaaa ttgcagtaat gactaacgaa
      841 aacacacaaa aattagccga gcgaatcgcg cttctattgc aggaaacaga acagccgaaa
      901 gacgattttt cggctttgca gacaacgctc gaaaaaatta atcgaagact ggacaaaatc
      961 gaatcgcagt ttgccgccca gaatttaaat aaaactcagc actcggcgct tgccgccgcg
     1021 catttaaagc cgcatcccag ccaggaaaaa tttgcgattg acgaagcggt cgttaacgaa
     1081 atactcgaac attttgaaaa agaaaaggct tgcacgttcg agccgaacgg caagccttgt
     1141 gaccactgcg cgatgtgcag ctcgcgcggt ttctagttta tttgttcgcg tcgcggcgaa
     1201 tcgaattcgg gtcgcctaaa ttcggcgctt gacgcgagcc ttcgtcggct ttgaagaaag
     1261 gctcttcctt aaatccgaac atactcgccg tgataaccgc cgggaacgaa ttccgcgccg
     1321 tgttatattt cgttaccgcg tcattataat cgagccgcgc cgtattgata cggttttccg
     1381 ttcccgaaag ctcgtcctga actttcataa acgcttcgtt cgagcgcaaa accggataac
     1441 tttcctgcaa gctcaaaagc cttccgattg ttccgccgaa actgttgttc gcgtcgatca
     1501 ccgcttgttt ttgttccggc gttttatcgc cgtcagcgcc ggcgggcggc gcctgcacgg
     1561 cgtttaaaag acgcgagcgc gcatcggcaa tctgcccgaa aacttcctgt tcctgaatcg
     1621 ccgacatctg tgcggcttta accagattcg gaatcaaatc ggcgcgccgc tgcaaattgc
     1681 tttcgacatt cgcccatttt cctctcacgc cttgttgctg cgcagttaaa tcgttgtagc
     1741 tgcaaccgct tgcgccgaaa gcgacaagca gcgcgatggt taaaagaatt gtttttctca
     1801 tggttttaat ctccttccg
//
LOCUS       DASWNE010000656         3903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_514656, whole genome shotgun sequence.
ACCESSION   DASWNE010000656 DASWNE010000000
VERSION     DASWNE010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514656"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /gene="hprK"
                     /locus_tag="VGB68_12815"
     CDS             <1..940
                     /gene="hprK"
                     /locus_tag="VGB68_12815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HEY0050168.1"
                     /translation="IDISEFVEKSPAQFQIEVLGGRESLRSRRITSARIQKLGLALAG
                     FSHYIHEGRVQIVGQSEISYLNQLSVEKRIEAIRNLDLKKICCVLLTKNLEPPPEFVR
                     IAEEFDLPVLRTPQVSSAAIVVVSNFLQEMLAPRTMLHGVLMGMYGLGVFLQGESGIG
                     KSECALDLITRGHHLISDDAVWVKKIGGVLEGSSPELTYEHLEIRGLGIINIRDLFGV
                     SAIGKQKKIDLCIELKKWVDLKEIERLGLETQEEEIFGVRIPKFVLPVSSGRNVSTLV
                     ETAVRVHLLRTTGYDAAQKLIEKHTEILSAATIVND"
     gene            944..1834
                     /gene="rapZ"
                     /locus_tag="VGB68_12820"
     CDS             944..1834
                     /gene="rapZ"
                     /locus_tag="VGB68_12820"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEY0050169.1"
                     /translation="MKKNQQKGVPNFVIVTGLSGSGMSSATNAFEDLGYFCVDNLPVT
                     MLPPFARLLQPNEEEKPAIEKAALVIDIRERHFLSDFPSELKKLAKKNLEAFVLFFEA
                     SDEVLQRRFSETRRPHPFDQGDGLLTAIQAEREALQNVRKTADLIVDTSEHTVHTLRK
                     FLIERFGQTEEGTPLHVQVVSFGHKYGVPRQVDLLFDVRHLPNPYFVPELKHLPGTDE
                     NVIKFLDGQEEVHETTNRFADLLAYLLPLYKREGKSYLTIGVGCTGGRHRSVRVAYNL
                     GNFLKEKGFDVSVSHRDVQK"
     gene            1922..2350
                     /locus_tag="VGB68_12825"
     CDS             1922..2350
                     /locus_tag="VGB68_12825"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEY0050170.1"
                     /translation="MSEIKKIGGVIVSHGQLANELLSAAETVVGELSHIAAVSIGWHD
                     DVETAKDEIARAIKKVSGGRGVLLLTDMFGGTPTNISAMFIADGEIEIVTGVNLPMVI
                     KLASQNQDDATLQAMAKDVEAQGKQSIYCASELLAPQKLK"
     gene            2352..2624
                     /locus_tag="VGB68_12830"
     CDS             2352..2624
                     /locus_tag="VGB68_12830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEY0050171.1"
                     /translation="MPEANIKIINQLGLHARAAAQLVRLAGTFKSRVTLMRLDNLVAA
                     DAKSILSVLTLAASKGTELKLTVEGVDEQAAFEAVKKIFANGFGEI"
     gene            2628..3590
                     /gene="rocF"
                     /locus_tag="VGB68_12835"
     CDS             2628..3590
                     /gene="rocF"
                     /locus_tag="VGB68_12835"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HEY0050172.1"
                     /translation="MHSSDRDYPGKDKKVGIIGIPLGFAAGQAGSELGAMAIRLADIR
                     GKRLVEHIRDLGYGVNDYGDLMLAHPQAAANIEDNPKYLPEMLAACEVMSEAVSQIFQ
                     AGELPVILGGDHSVAIGTFSGISSFFRGEDKEIGLIWFDAHADMNTPETSPSGNLHGM
                     PLAILLGIGEEKLVNAGGFAPKLNPEFLAHVGARDLDLGERRLIRELGIREQFFTMSD
                     IDKRGMTDCVKDAIKVASRAKDGFVVTFDVDVIDPRFAPGSGTLVRGGITYREAHLAL
                     EIIAEHGGMRSFEIVEVNPTLDTANVTVELAGELILSALGKTIL"
     gene            3703..>3903
                     /locus_tag="VGB68_12840"
     CDS             3703..>3903
                     /locus_tag="VGB68_12840"
                     /inference="COORDINATES: protein motif:HMM:NF013942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050173.1"
                     /translation="MKTRIGVIFGGRSGEHEISIRSAQTVIEQIDKDKYEVVPIAISS
                     EGKWLNPVDALALLPSRTQEFLT"
BASE COUNT          973 a          936 c         1096 g          898 t
ORIGIN      
        1 catcgacatc agcgagtttg ttgaaaaatc tccagctcaa tttcaaatcg aggttttagg
       61 cggtcgggaa tctctgcgct cgcgccggat tacttcggcg cgaatacaaa agctggggct
      121 ggcgctcgcc ggattttctc attatattca cgaaggtcgc gtgcagattg tcgggcaaag
      181 cgagatttcc tacttaaatc agctgtcggt cgaaaaaaga atcgaagcca tcagaaatct
      241 cgatttgaaa aaaatctgct gcgttttgct gactaaaaat cttgagccgc cgccggagtt
      301 cgtccgcatc gccgaagaat tcgatttgcc cgttttgcgc acgccgcagg tcagctcggc
      361 tgccatcgtc gtcgtctcga attttttgca ggagatgctc gcgccgcgga cgatgctgca
      421 cggggttttg atgggaatgt acggcttggg cgtttttttg cagggcgaat cgggcatcgg
      481 gaaatccgaa tgcgcgctgg atttaatcac gcgcgggcat cacctgattt cggacgacgc
      541 cgtctgggtc aaaaaaatcg gcggcgtgct ggaaggcagt tcgcccgaat tgacttatga
      601 gcatttggaa atccgtggtc tgggcattat caatattcgc gatttattcg gagtttcggc
      661 aatcggcaaa cagaaaaaga ttgatttgtg tatcgagctg aaaaaatggg tagacctcaa
      721 ggaaatcgaa cggctcgggc tggaaacgca ggaagaagaa attttcggcg tcaggattcc
      781 gaaattcgtt ttgccggtca gctccggcag aaacgtctcg acgctggtgg agaccgccgt
      841 tcgcgtccat cttttgcgca cgacgggtta tgacgccgcc cagaaattga ttgaaaaaca
      901 cactgagatt ttatccgccg cgacaatagt aaacgactga tttatgaaga aaaaccagca
      961 gaaaggagtt ccgaattttg taatcgtcac gggtctttcg gggtcgggaa tgtcttcggc
     1021 gaccaacgcg tttgaagatt taggatattt ctgcgtcgat aacctgccgg tgacgatgct
     1081 tccgcctttc gcgcggcttt tgcagccgaa cgaagaagaa aaaccggcga ttgaaaaagc
     1141 cgcgctggtc atcgatattc gcgaacggca ttttctttcg gactttccga gcgaactgaa
     1201 aaaactggcg aagaaaaatc ttgaggcgtt cgtcttgttt tttgaagcat ccgacgaagt
     1261 tttgcagcgc cgcttcagcg agacgcgccg cccgcacccg tttgaccagg gagacggttt
     1321 gctgacggcg attcaagccg agcgcgaggc attgcaaaac gttcggaaaa cggcggattt
     1381 gattgtcgat acatcggagc acacggttca cacgctcaga aaatttctca tcgaaagatt
     1441 cgggcagacc gaagaaggaa cgccgcttca cgtgcaggtc gtcagcttcg gtcataaata
     1501 cggcgttccg cgccaggtcg atttgctttt cgacgtgcgg catctgccga atccgtattt
     1561 tgtgccggaa ctcaaacatc tgccgggaac ggacgaaaac gttataaaat ttctggacgg
     1621 gcaggaagaa gttcacgaaa cgaccaatag atttgccgat ttgctggctt atctgctgcc
     1681 gctttacaag cgcgaaggca aatcttattt aaccatcggc gtcggctgca ccggcgggcg
     1741 acatcgctcg gtgcgcgtgg cgtataatct gggaaatttt ttgaaggaaa aaggatttga
     1801 cgtcagcgta tcgcatcgcg acgttcaaaa atagaaactg ttcattagtc ctttgccggt
     1861 tgagaaataa atttagactg aatttaaatt caacaaacaa aaggacgaag gacgattaaa
     1921 tatgtctgaa attaaaaaaa tcggcggcgt catcgtctcg cacgggcagc tggcgaacga
     1981 gctgctctcg gcggcggaaa cggtcgtcgg cgaattgagt cacatcgcgg cggtttctat
     2041 cggctggcac gacgacgtcg aaacggcgaa agacgaaatc gcccgcgcca tcaaaaaagt
     2101 ttccggcggc aggggcgttc tgcttttaac cgatatgttc ggcggcacgc cgacgaatat
     2161 ttccgcgatg tttatcgccg acggcgaaat cgaaatcgtc accggcgtca acctgccgat
     2221 ggtgattaag ctggcttcac aaaatcagga cgacgcgaca ttgcaggcta tggcgaagga
     2281 cgtcgaggcg cagggcaagc agtcgattta ctgcgcgagc gagctgctcg ccccgcaaaa
     2341 actaaaataa gatgcccgag gcgaacatca aaatcatcaa tcagctcggg cttcacgccc
     2401 gcgccgccgc acaactggtg cggcttgccg gaacttttaa aagccgggtg acgctgatga
     2461 ggctcgacaa tctggtggcc gccgacgcga aatcaatcct cagcgtcctg acgctggcgg
     2521 cgagcaaggg cacggaattg aaattaaccg tcgaaggcgt tgacgaacaa gccgccttcg
     2581 aagcggtaaa aaaaatattt gcaaacggat ttggtgaaat ttaatttatg cattcatctg
     2641 accgggatta tccgggaaaa gataaaaaag taggaatcat cggcataccg ctcggttttg
     2701 ccgccggaca agccggaagc gaattaggcg cgatggcgat tcgcctggct gatattcgcg
     2761 gaaaacgact cgtcgagcat atcagggatt tgggctacgg ggtcaacgat tatggcgatt
     2821 tgatgctcgc tcacccgcaa gccgcggcaa atatagagga caatccgaaa tatctgccgg
     2881 aaatgcttgc cgcctgcgaa gtgatgtcgg aagcggtcag tcaaattttt caagccggcg
     2941 agcttcccgt cattctcggc ggcgaccatt cggttgcgat tggaactttt tccggcattt
     3001 cttcattttt ccgcggcgaa gacaaggaaa tcggcttaat ctggtttgac gctcacgccg
     3061 atatgaacac gccggagacg tcgccgtcgg gaaatctgca cggaatgccg ctggcgattc
     3121 ttttaggaat cggcgaggaa aaactggtca atgcgggcgg ctttgcgccg aaactgaatc
     3181 ctgaatttct ggcgcacgtc ggcgcgcgcg atttggattt gggcgagaga cgattgattc
     3241 gcgagctcgg catccgcgag cagtttttta cgatgagcga cattgataaa cgcggaatga
     3301 ccgattgcgt caaagatgcg attaaggtcg cttcaagagc gaaagacggt ttcgtcgtaa
     3361 catttgacgt ggacgttatc gacccgcgtt tcgcgcccgg ttcgggaacg ctggtgcgcg
     3421 gcggcatcac gtatcgcgaa gcgcatctgg cgctggaaat tatcgccgaa cacggcggaa
     3481 tgcgctcgtt tgaaatcgtc gaggtcaatc ccacactcga cacggcgaac gttacggtcg
     3541 agctcgccgg agagcttatt ttgtcggcgc ttgggaaaac tattctttga agttgcggaa
     3601 agctgcagat agttgcagag ttgcgggaag ttagaagaat attcttcaca ccgcaattat
     3661 ctgcagcttt ccgcaactat ctgaaacttc ctgtaaactt ttatgaagac tcgtatcggt
     3721 gtaatttttg gcggtcgttc gggcgagcac gaaatctcga ttcgctcggc gcagaccgtg
     3781 attgaacaga tagataaaga taaatatgaa gtcgtgccga ttgccatctc cagtgaagga
     3841 aaatggctca atccggtcga tgcgctggct ttgcttccgt cgcggacgca ggagtttctg
     3901 acg
//
LOCUS       DASWNE010000657         3679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_91956, whole genome shotgun sequence.
ACCESSION   DASWNE010000657 DASWNE010000000
VERSION     DASWNE010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91956"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VGB68_12845"
     CDS             <1..382
                     /locus_tag="VGB68_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050174.1"
                     /translation="VNPDDGFTLRETVAEYHQIITLQASEMKHLGIKKPLPMTETQEV
                     TLYGGGALIFDEFGRVKFHIRNRLLNPERQTRRLEYLWRYGEFQLSAQEKNSSRRFAQ
                     MHRARFNHIKEEGVADDNYLEKHR"
     gene            354..1733
                     /locus_tag="VGB68_12850"
     CDS             354..1733
                     /locus_tag="VGB68_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050175.1"
                     /translation="MTTISKNTDSAKQKSNSGSITKSSKPDKLTLCSYQMGFGDCYLL
                     TFHYGGEKRHILIDFGSTGKPADAPSDLMLRVATDIRQRCDGKLHIVVATHRHKDHIS
                     GFATNEAGNASGDIIRECAPDLVIQPWTEDPEAPVDATNSTTLSSGKSIAFSETLLHM
                     QTVAELIKNEAHSLRACDEMAALAEQLAFLGEDNISNVSAIKNLIKMSSGGRGRYVCA
                     DFKNPLNLDNLLPGVTVRVLGPPNLQQSKEITKMAHDDAAEFWQLSANAGQEGVGDGA
                     AKPLFQYIAKREAAQLIPPHTRWFIRRMDSMRGTQMLEIVRILDSVMNNTSVILHFEV
                     GGKKLLFPGDAQIENWSYALQEARNNPKLRELLEDVDVFKVGHHGSRNANPKSLWEMF
                     KKKNSAPSDKRLRTFVSTMEGKHGHAEKKTEVPRASLVEALQSQSSYFSTQELKKDEL
                     SREFTINLP"
     gene            complement(1907..2941)
                     /locus_tag="VGB68_12855"
     CDS             complement(1907..2941)
                     /locus_tag="VGB68_12855"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HEY0050176.1"
                     /translation="MIDLRSDTVTRPTPAMRRAMAEAEVGDDVYGEDPTVNLLQERAA
                     EMFGKQAALFVPTGSMGNQIAVKLHTRSGDEIIIEERGHIFNYELGAPSLIAGVVVRA
                     VRSQSPTGMLTWDEIAAALRINQVYYVSPTGLICLENTHNFGGGSVMSAGETTEICER
                     AHALDLPVHLDGARIFNAATALGESVADLSKHCDSVQFCLSKALGAPVGSMLLGEKDF
                     IEEARVWRKRLGGGMRQVGVLAAAGLVALEETPKLLHIDHENARALAEGAANLKGVSI
                     DAGKVVTNIVIFDVAASGKTSAEIVARLKEEEILAIGFGSAIRMVTHYEVSREDIEKT
                     IKALEKVLGE"
     gene            complement(2964..>3679)
                     /locus_tag="VGB68_12860"
     CDS             complement(2964..>3679)
                     /locus_tag="VGB68_12860"
                     /inference="COORDINATES: protein motif:HMM:NF019615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0050177.1"
                     /translation="AAKSAAHALFCEKVYGVNLCQHGLADVFQLNKLLEALRLSADER
                     VLDLGCGNGFITEYLQSETGAFFHGVDISAEAIRQARRRARNERLAFSVGNMNRLQFE
                     PRAFDCAVSIDTLYYVDNPEETLKQIVSILKPGGRIGIFYTQWINDLADREYLLPGNT
                     YLAKLSEKYDLKFRALDLTSNEAEHWRKKVEVLEEMKPQFEREGNLNLYNYRYTEAAR
                     YANWDLEKRSRYLYHITLS"
BASE COUNT          874 a         1027 c          943 g          835 t
ORIGIN      
        1 cgtgaatccc gacgacggat ttacgctgag agagaccgtg gcggaatatc accagattat
       61 cacgcttcag gcttcggaga tgaaacatct cggcatcaaa aagccgctgc ctatgacaga
      121 gactcaggaa gtgacgcttt acggcggcgg ggcgttgatt tttgacgaat tcggacgagt
      181 caaattccat attcgcaacc ggctcttaaa tcccgagcgg cagacgcgcc gccttgaata
      241 tctctggaga tacggggaat tccaattaag cgctcaggaa aagaactctt cgagacgctt
      301 cgcacaaatg caccgcgcac gattcaatca cattaaagaa gaaggagtgg cagatgacaa
      361 ctatctcgaa aaacaccgat agcgcaaaac aaaagagcaa ttccggctcg attactaaaa
      421 gctcgaaacc cgataaactg accttgtgca gttaccagat gggcttcggc gattgttatc
      481 tgctgacttt tcattacggc ggcgagaagc ggcacatact catagatttc ggcagtacgg
      541 gaaagcccgc cgacgctcct tcggatttga tgctgcgcgt ggcgacggac atcaggcaga
      601 ggtgcgacgg aaaattgcat atcgtcgtcg ccacgcaccg ccacaaagac cacatcagcg
      661 gctttgccac taacgaagcc ggaaatgcgt cgggcgacat cattcgcgag tgcgctcccg
      721 acctggttat ccagccctgg acggaagacc ccgaagcgcc ggttgacgct actaactcca
      781 ccactttgtc gtcgggcaaa agcattgcct tttccgaaac actgctccat atgcagacgg
      841 tcgccgagct gatcaaaaat gaagcgcatt ctctgcgcgc gtgcgacgag atggcggctc
      901 tcgccgagca gctcgctttt ctcggcgagg ataatatcag caacgtttcg gcaattaaaa
      961 atttaataaa aatgtcgagc ggcgggcgcg ggcgatatgt ctgcgccgat ttcaaaaatc
     1021 cgctgaatct ggataattta ctgccgggcg tcacggtgcg cgtgctggga ccgcccaatc
     1081 ttcagcaatc taaagaaatc accaaaatgg ctcacgacga tgcggctgaa ttctggcaat
     1141 tgagcgccaa tgccgggcag gagggcgtcg gtgacggagc agcgaagcct ctcttccagt
     1201 atatcgccaa gcgcgaggct gcgcagctga taccgccgca cactcgatgg ttcatccgtc
     1261 gtatggattc gatgcgcggc actcagatgc ttgaaatcgt tcgcattctc gacagcgtga
     1321 tgaataatac gagcgtcatt ctccacttcg aggtcggagg caaaaaatta ctctttcccg
     1381 gcgacgcgca aatagaaaac tggtcttatg ctttgcagga agccagaaat aatccgaagc
     1441 tcagagagct tctcgaagac gtggacgtgt ttaaagtcgg tcatcacggc agccgcaacg
     1501 ccaatccgaa atcgctctgg gagatgttta aaaagaaaaa ctccgcgcct tccgacaagc
     1561 gcctccggac attcgtttca actatggagg gcaaacacgg acacgccgag aaaaaaaccg
     1621 aggtgccgcg cgcgtcgctg gtcgaagcgc tgcaatcgca gagcagctat ttttccactc
     1681 aggagcttaa aaaggatgaa ttgagccggg agtttactat taacctgccg taattccggc
     1741 atctatagat tcggcgcggg ataatcctga atcgagcgga ggctggcttc gccgcagttt
     1801 tcaaattgat tttcccgcgc cgggaaatat tattaaaaga tggacaatac cgatatcaca
     1861 accggcggag ggcaataaat taatgcgtat ttcccgcgcg tccgttttat tctcctaaaa
     1921 ctttttccag cgcttttatc gttttttcga tgtcttcgcg cgaaacctcg tagtgcgtga
     1981 ccatccgaat cgcgctgccg aaaccgatag ctaagatttc ttcttctttc aaccgcgcaa
     2041 caatctcggc tgaagttttg ccggacgccg cgacgtcgaa aatcacgata ttggtcacga
     2101 ctttcccggc gtcgatagaa acgcctttca ggttcgccgc gccttccgcc aacgcccgcg
     2161 cgttttcgtg gtcgatgtga agcagtttcg gcgtctcttc cagcgcgacg agccctgccg
     2221 ccgccagaac gccgacctga cgcattccgc cgccgagccg tttgcgccag acgcgcgctt
     2281 cttcgataaa atctttttcg cccagaagca tcgagccgac gggcgcgccg agcgctttcg
     2341 aaagacaaaa ctgcaccgaa tcgcaatgtt ttgacaaatc ggcgaccgat tcgccgagcg
     2401 ccgtcgccgc gttgaaaatt cgcgcgccgt ccaggtgaac gggcaaatcg agcgcgtgcg
     2461 cccgctcgca gatttcggtg gtttctccgg cgctcatcac gcttccgccg ccgaaattgt
     2521 gcgtgttttc cagacaaatc aatccggtcg gcgatacgta atacacctga ttgatacgca
     2581 gcgccgcggc gatttcgtcc caggtcagca ttccggtcgg cgactggctg cgcacggcgc
     2641 gcacgacgac gcccgcgata agcgacggcg cgcccaattc gtaattaaaa atgtgcccgc
     2701 gttcttcgat gataatttcg tcgccgctcc gcgtgtgcag cttgacggcg atttgatttc
     2761 ccatcgagcc ggtcggcacg aaaagcgcgg cttgcttgcc gaacatctcg gcggcgcgct
     2821 cctggagcag attaaccgtc gggtcttcgc cgtaaacgtc gtcgccgact tccgcttcag
     2881 ccatcgcgcg gcgcatcgcg ggcgtcggtc tggtcactgt atcgcttctt aaatcaatca
     2941 tttccgctaa ttaaataatg cttttatgac aatgttatat ggtaaagata gcggctgcgt
     3001 ttttctaaat cccaattcgc gtagcgcgcc gcttccgtat acctgtaatt atacaaattc
     3061 agatttcctt cccgctcgaa ctgcggtttc atctcttcta aaacctcgac tttttttcgc
     3121 cagtgttcgg cttcgttcga cgtcaaatcg agcgcccgaa atttcaaatc gtatttttcc
     3181 gaaagttttg ccagatacgt gtttccgggc agcaaatatt cgcggtcggc gagatcgtta
     3241 atccactgcg tgtaaaatat tccgattcgc ccgccgggtt tcagaatcga aacgatttgt
     3301 ttcagagttt cttccggatt atcgacgtaa taaagcgtat ctatggaaac ggcgcaatcg
     3361 aaagcgcgcg gctcgaattg cagacgattc atatttccga cgctgaaagc cagccgctcg
     3421 tttcgcgctc tacggcgcgc ctgccggatc gcttcggcgg aaatatcgac gccgtgaaaa
     3481 aacgcgcccg tttcgctttg cagatattcg gtgataaaac cgttgccgca gcctaaatcc
     3541 aggacgcgct cgtccgcgct taaccgcaag gcttcgagca gtttatttaa ttgaaaaaca
     3601 tccgccaatc cgtgctggca gagatttacg ccgtaaacct tttcgcaaaa aagcgcgtgc
     3661 gccgcgcttt tggctgccg
//
LOCUS       DASWNE010000658         3618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_58389, whole genome shotgun sequence.
ACCESSION   DASWNE010000658 DASWNE010000000
VERSION     DASWNE010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58389"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="VGB68_12865"
     CDS             complement(<1..819)
                     /locus_tag="VGB68_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050178.1"
                     /translation="MRKLIFILGFLVLSVFSITISLKVAARVENADQEMQEDEPDLPG
                     SSKMDKEDFLRLRADHTATLQGMDTAKTDSRAKSIEQMEQGERARDQRRAALNQPLAP
                     MWIPLGPAPIPISATTSNSGRVSAIAVHPTNPNIVYVGTAQGGLYRSVNGGGTWTPLL
                     DGALTLAIGAVAIAPSDPSTIFVGTGESSLCSSGCYIGVGLYRITNADSNPVVSPVLN
                     KDAANNDVFTGRAVSEIIVHPTDPNILFTTTTSGIAGIGASTTGLTLPAAGLYRT"
     gene            complement(1262..2434)
                     /locus_tag="VGB68_12870"
     CDS             complement(1262..2434)
                     /locus_tag="VGB68_12870"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEY0050179.1"
                     /translation="MKKIIKIILSIFVVLLIAFAAVFFFVLPSKIEADLNPVSNRPPY
                     RIGEKARALHEKLFIADLHADSLLWNRNLLEKSERGQADVPRLLEGNVALQAFTVVTK
                     SPRGLNIERNADETDNIYWLALAQRQPLENLSSLTKRALWQASKLHEYAEKSNGKLVV
                     IKTKSDLRAFVERRRSEKIIGGWLGIEGAHALDGKPENVDILFDAGFRMMSPSHFFDN
                     DIGGSAHGVEKYGLTEKGREMIRRMEAKGMLVDVAHASPKVIEDVLRVATEPVVVSHT
                     GVKGTCSNQRNLSDEQLRAIAGTGGVIGIGFWDTAVCGEDAQSIAKAIRYAANAVGAQ
                     HVALGSDFDGSVKVPFDTSGVALITEALLNENFSEDEIAGIMGGNVLRLLSEQLPD"
     gene            complement(2490..3572)
                     /locus_tag="VGB68_12875"
     CDS             complement(2490..3572)
                     /locus_tag="VGB68_12875"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY0050180.1"
                     /translation="MAKKVGIGIIGTGFARTVQIPSFQKIEGAQIVSIASGRRENAEK
                     AARDFNIPHWTGDWRETIERADADLICITTPPPFHREMTLYALERGRHILCEKPMAMN
                     AGEALEMWEKSKEKNVLAVIDHELRYLNGRRRAHSMIRAGEIGKIRHAKYNFRAPHRG
                     NPDAPWNWWSDEKQGGGALGAIVSHIFDSLRWFTGAEIRQVFCQLQTHVKRRKDSGGE
                     RREVTSDDESNLILRFADNELTEDATANISASMVEYPMYQNRIELFGTSGALRVEYDG
                     ELFIGKPDDSGWQKIEVDLNERIETAANTGWNSGFIAFAEEIVKALQAGETTVENAAT
                     FEDGYKIQIALDAARESSASGCAVKI"
BASE COUNT          772 a         1041 c          908 g          897 t
ORIGIN      
        1 cgtgcggtaa agtccggctg cgggcaaggt caatcccgtc gtgcttgcgc cgatacccgc
       61 aataccgctg gtcgtcgtcg taaataaaat attcgggtcg gtcggatgaa cgatgatttc
      121 gctgaccgcg cgcccggtga aaacgtcatt gttcgccgcg tctttgttga gaaccggcga
      181 gaccaccgga tttgaatccg cgttggtgat gcgataaagt ccgacgccga tatagcaacc
      241 gctgctgcac aaactggatt ctcccgtgcc gacgaaaatc gtcgaagggt cggacggcgc
      301 aatggcgacc gcgccgattg ccagcgtcaa cgcgccgtcg agcagcggcg tccatgttcc
      361 gccgccgttg accgaacgat acaaaccgcc ctgcgccgtg ccgacataga cgatattcgg
      421 attggtcggg tgcaccgcga ttgccgaaac gcgcccgctg ttcgaggtcg tcgcgctgat
      481 cggaatcggc gccggaccga gcggtatcca catcggagcg agcggctgat tcagagcggc
      541 gcggcgctgg tctcgcgccc tttcgccctg ttccatctgc tcgatggatt tggcgcgcga
      601 atccgttttg gcagtatcca taccttgcag cgtggcggta tggtcagccc gcaggcgcag
      661 gaagtcttcc ttgtccattt tcgagctgcc cggcaggtcg ggctcgtctt cctgcatttc
      721 ctggtctgcg ttttcaaccc gcgccgcgac tttcaaactg attgtaattg aaaaaacgct
      781 cagtacgaga aagcctagaa taaatattaa ttttcgcatt atgatttctc ccggtagaaa
      841 ttttcgttcg tcctgttaat gaacggttat cgtaatttgt cttgaattac aacggaatcc
      901 gaagctgaca caaaagccgc gccggttcga caaaacagtt tcaaaacctt aaagattttt
      961 gattatgtga acgggcggac aaagaaacag agactcttag cttgaccgct ttcagctaaa
     1021 aaccggcgcg ttttatttac tactttcgcg cgccgcgcgc cttataagcg cgatttaaga
     1081 ttgttcggag aaagcgatta gccgagaatt gttataattt agcactgctt ttggattttc
     1141 gaaagctgat tgcgcctctt ttacaaagga tttttttctt gtttccgcct gaagcgccgt
     1201 ttgcgggcgc gtccgattat taataatgcg tcggcgcatt ttgttttttt gagtccggat
     1261 ttcaatcggg cagttgttcg gaaagcagtc tcaggacgtt tccgcccata attccggcga
     1321 tttcgtcttc ggaaaaattt tcattcagta atgcttccgt tatcaaagcg acgccgctcg
     1381 tatcgaacgg aactttcacc gagccgtcaa aatccgagcc gagcgcaacg tgctgcgcgc
     1441 cgaccgcatt tgccgcgtaa cgaatagctt tcgcaatact ctgcgcgtct tcgccgcaaa
     1501 ccgccgtatc ccagaaaccg atgccgatga cgccgcccgt cccggcgatt gcccgcaatt
     1561 gctcgtcgct caaatttcgc tgattgctgc aagttccctt tacgcctgta tgtgaaacga
     1621 caacgggctc ggtcgctacg cgtaaaacgt cttcgataac tttcggcgaa gcgtgcgcga
     1681 catcgaccag cattcctttg gcttccatcc ggcgaatcat ttccctgccc ttttcggtca
     1741 agccgtattt ctcgacgccg tgcgccgagc cgccgatgtc gttgtcgaaa aaatgcgacg
     1801 gcgacatcat ccgaaaaccc gcgtcgaaca gaatatcgac gttctccggc ttgccgtcca
     1861 gagcgtgcgc gccttcgatg ccgagccagc cgccgattat cttttcgctt cggcggcgct
     1921 cgacaaatgc gcgcaaatcg ctttttgttt tgatgacgac cagtttcccg ttcgattttt
     1981 cggcgtattc gtgaagcttc gacgcctgcc agagcgcgcg tttcgtcagg ctcgaaagat
     2041 tttccagcgg ctgtctctgc gccagcgcca gccagtaaat attatcggtt tcatcggcgt
     2101 ttcgctcgat attcaaaccg cgcggcgatt tcgtgacgac cgtgaaagcc tgcaaagcga
     2161 cgttgccttc caaaaggcgc gggacatccg cctgcccgcg ctcgcttttt tccagcagat
     2221 ttcgattcca taaaagcgaa tcggcgtgca gatcggcgat gaaaagcttc tcgtgcaggg
     2281 cgcgcgcttt ttcgccgatt cgatacggcg gtcgattgct aaccggattc aaatcggctt
     2341 cgattttcga gggcaaaacg aaaaagaaaa ctgccgcaaa cgcgatgagc aaaacgacga
     2401 agattgataa tataattttt ataattttct tcatcgcaag ttatcgagcc gacgcgaagc
     2461 ggctttatct gcaaacaatt tccgacgctt caaattttta ccgcgcagcc gctcgcgctc
     2521 gattcgcgcg ccgcgtccag cgcaatctga attttataac cgtcttcaaa cgtcgcggcg
     2581 ttttccaccg tcgtttcgcc cgcctgcaaa gcctttacga tttcttcggc gaaagcgata
     2641 aaaccgctgt tccagcccgt attcgccgcc gtttcgattc gctcgtttaa atccacctca
     2701 attttttgcc aaccggaatc gtccggtttt ccgataaaaa gctcgccgtc gtattcgacg
     2761 cgcagcgcgc cgctcgtgcc gaataattcg atgcggtttt gatacatcgg atattcgacc
     2821 atcgacgccg aaatgttggc ggtcgcgtct tcggtcagct cattgtcggc aaatcgcaga
     2881 atcaaattcg attcgtcgtc gctcgtcacc tcgcgccgct cgccgccgga atctttgcgc
     2941 cgcttgacgt gcgtctgcaa ttgacagaaa acctgccgga tttccgcgcc cgtaaaccag
     3001 cgcaaagaat cgaaaatgtg cgagacgatt gcgcccagcg cgccgccgcc ctgtttttcg
     3061 tcgctccacc agttccacgg cgcgtcggga tttccgcgat gcggcgcgcg aaaattgtat
     3121 ttggcgtgac gaatttttcc gatttcgccg gcgcgaatca ttgaatgcgc cctgcgtctg
     3181 ccgttcaaat aacgcaattc gtggtcaatc actgcgagca cgtttttttc tttcgatttt
     3241 tcccacattt cgagcgcctc gcccgcgttc atcgccatcg gcttttcgca caaaatatgt
     3301 cgcccgcgct ccagagcgta cagcgtcatc tcgcggtgaa aaggcggcgg cgtggtgatg
     3361 caaatcaaat ccgcgtcggc tctttcgatt gtctcgcgcc aatcgcccgt ccaatgcgga
     3421 atattaaaat cccgcgccgc tttttcggcg ttctcgcgcc gcccgctggc gatcgaaaca
     3481 atctgcgcgc cttcgatttt ttgaaaggaa ggaatctgca ccgtccgcgc aaaacccgtc
     3541 ccgataatcc cgattccgac ttttttagcc ataaaaaaat taaaacacgg cgaaaatgca
     3601 aaacgcaaaa tgcaaaac
//
LOCUS       DASWNE010000659         1561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_305769, whole genome shotgun sequence.
ACCESSION   DASWNE010000659 DASWNE010000000
VERSION     DASWNE010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305769"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(317..1504)
                     /gene="nifS"
                     /locus_tag="VGB68_12880"
     CDS             complement(317..1504)
                     /gene="nifS"
                     /locus_tag="VGB68_12880"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR03402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HEY0050181.1"
                     /translation="MFRRVYLDNSATTPVDEAVVTEMLPFLTEKFGNASSIHFFGQEA
                     RAAVDKARHQVSEILNARPNEIVFTSGGTEADNLAVRGLVEANQKYGRHIITSQIEHS
                     AVKNVCQDLEKRGFEVTYLPVYEDGLVRIEDVRGAIRQDTVLITIMTANNEIGTIQPV
                     EEIGRFVRELRAEGGKIWFHTDAVQAIGRMKTDVEEIGCDLLSMSAHKLYAPKGVGAL
                     YVRRGVRLQQQNIGGHQERERRGGTESVPHIVAFGKACELAQKTLSERISYVRSLRDR
                     FEQGVAERITSVVFNGDREKRLPHLSNISFRFIEGEGLLINLDMQGVAVSTGSACSSG
                     SLEPSPVIRALGRTDELARGAIRFSFGKDNTPEEVEYVLETLPRAVENLRKLSPAFVN
                     KKC"
BASE COUNT          327 a          412 c          431 g          391 t
ORIGIN      
        1 ttcttttgat agatgccgag tctaaactta agcagaaagc aggcagttat ttactctata
       61 tcggcggtgt tattgctctc ttccttattt tcaaatcatt attaatttgg tagttttctc
      121 tatttaaagc ttgaatcaat aatttcgctt ttggaattaa aagtgaaggt gtgtctacga
      181 cgcgcagccc actggcaggc agcatgcgcg tcggcggggg cgcagctttc ttttggtgga
      241 agagaaacgc gctcttgttt tttgagccgg gacgggaagt ttatttgatt ttccggttcg
      301 gctcaaaacg cgtatttcaa catttcttat taacgaatgc gggcgaaagc ttgcggagat
      361 tttcgaccgc gcggggcaga gtttccagaa cgtattcgac ctcttccggc gtgttgtcct
      421 tgccgaagct gaagcgaatc gcgccgcgtg ccagctcgtc ggttctgccg agcgcgcgaa
      481 tcacgggcga cggctcgagc gagccggacg agcaagccga gccggtcgag acggcgacgc
      541 cctgcatatc gagatttatc agcaagcctt cgccttcgat aaaacgaaaa gagatgttgg
      601 aaagatgcgg caggcgtttt tcgcggtcgc cgttaaatac gacgctcgta attcgctcgg
      661 cgacgccctg ctcgaatctg tcgcgcaagc tgcggacgta gctgattctt tcgcttagag
      721 ttttttgcgc cagctcgcaa gccttgccga aggcgacgat gtgcgggacg gattcggtgc
      781 cgccgcgccg ctcgcgttcc tgatgcccgc cgatgttttg ttgctggagg cgcacgccgc
      841 gccggacata taaagctccg acgcctttcg gcgcgtagag cttgtgcgcc gacatcgaaa
      901 gcaaatcgca gccgatttct tcaacgtccg ttttcatccg cccgattgcc tgcacggcgt
      961 ccgtgtgaaa ccagattttt ccgccttcgg cgcgcaattc gcgcacaaac ctgccgattt
     1021 cctcgacggg ctgaatcgtg ccgatttcgt tattggcggt cataatcgtg attaagaccg
     1081 tatcctggcg aatcgcgccg cgcacgtcct cgattcgcac caaaccgtct tcgtaaaccg
     1141 gcaggtatgt cacttcaaag ccgcgctttt ccaaatcctg acagacgttt ttgaccgccg
     1201 aatgctcgat ttgcgaagtg attatgtgac gaccgtattt ctgattcgcc tcgaccagac
     1261 cgcgtacggc gagattgtcg gcttccgtgc cgccggacgt aaaaacgatt tcgttcgggc
     1321 gcgcgttgag gatttcagag acttgatggc gggctttatc gacggcggcg cgcgcttcct
     1381 gaccgaaaaa atggatgctc gaagcattgc cgaacttttc ggtcagaaac ggaagcatct
     1441 cggtgaccac cgcctcgtca accggcgtgg tcgcgctgtt atccagataa acgcggcgaa
     1501 acatactgcg attttaccac gaagaaagtg gtaaacggta aacggtaagt ggtaagttcg
     1561 t
//
LOCUS       DASWNE010000660         1738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_271041, whole genome shotgun sequence.
ACCESSION   DASWNE010000660 DASWNE010000000
VERSION     DASWNE010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271041"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VGB68_12885"
     CDS             <1..615
                     /locus_tag="VGB68_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050182.1"
                     /translation="ITEGKYFNFAVDDVDKVQTNANVMAEAMSRAGYGLRDKVDIFLS
                     ANHIYVPSANLIGDDTTPIVLTKDNFYDHLVDLKVKLTEANIPEEGRFAIIPAFGEGL
                     LLKDDRFVAAGTSTSDNVIRNGKIGRAAGMDIYVSNNTPNTAGTKYKIIAGHPMALTF
                     AEQIVKMEAYRPEKRFADAVKGLHVYGAKLVRPYAWAVLTANRS"
     gene            679..1068
                     /locus_tag="VGB68_12890"
     CDS             679..1068
                     /locus_tag="VGB68_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050183.1"
                     /translation="MANPVSLTINDLSANAGTSLPTAQSIDTNGTINLPANSLTDRLL
                     IEVVNNDDAALTVTVKAGTSAVAHTARDLVVALAASGGGATAQRMIGPFESARFVKPD
                     GSIDVQFQAASGSPAASVRVYRLPRQI"
     gene            1271..>1738
                     /locus_tag="VGB68_12895"
     CDS             1271..>1738
                     /locus_tag="VGB68_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050184.1"
                     /translation="MKAKLTLNNPLWQTAERRRILDKVVQESAVELEGEIKRKILTGT
                     KSGRTYRRGAITKAASQKNIALGLRRARGNPKRVFAGSRFHRASAPGESPANDTGSLA
                     NSLRSVSVKPLHSKVFTSKRYAETLDNPQKLNRPFFSQTAQKFKKRFKEQIKNA"
BASE COUNT          472 a          469 c          425 g          372 t
ORIGIN      
        1 atcaccgaag gcaaatactt caactttgcc gtcgacgacg ttgataaagt ccagacgaac
       61 gccaacgtga tggcggaagc gatgagccgc gccggctacg gtctgcgcga taaagtcgat
      121 attttcctct cggcgaatca catttacgtg ccgtcggcga accttatcgg cgacgacacg
      181 acgcccatcg tcctgacgaa agacaatttt tacgaccacc tggttgattt gaaagtaaaa
      241 ttaaccgaag ccaatatccc ggaagaaggg cgtttcgcca tcatcccggc gtttggcgaa
      301 ggcttgctgc tcaaagacga ccgtttcgtc gcggcgggaa cctcgacgtc tgacaatgtt
      361 attcgcaacg gcaaaatcgg gcgcgctgcg ggaatggaca tttacgtttc caacaacacg
      421 ccgaacaccg ccggcacgaa atacaaaatc atagcgggac acccgatggc gctgaccttc
      481 gccgagcaaa tcgttaaaat ggaagcctat cgcccggaaa aacgtttcgc cgatgccgta
      541 aaaggacttc acgtttacgg cgcaaagctc gtcagaccgt atgcctgggc tgtcttgacc
      601 gccaacagat cctaatcctt tttaagtcta cccggaggcg agctatagtt cctcgcctct
      661 ttttcacttt ttaaaattat ggctaatcct gtaagtctca ctatcaacga tttatcggcg
      721 aacgccggta ccagcctgcc gaccgcgcaa agcatcgata ccaacggcac gattaatctt
      781 ccggcaaact cgctcaccga ccgcttgctt atcgaagtcg tcaacaacga cgacgcggct
      841 ctgaccgtca ctgtcaaagc cggaacttcc gcagtcgctc acaccgcacg cgatttagtg
      901 gttgcgctcg cagcttccgg cggcggcgcg acggcgcaaa gaatgattgg accttttgaa
      961 tcggctcgtt tcgtcaaacc ggacggctcg atagacgttc aatttcaggc ggcttcgggc
     1021 tcgcccgcgg cttcggttcg cgtgtatcgc ctgccccgtc aaatctgacc tttatcaaat
     1081 agtgataagt gagaggtgat aagggaattc cctgattgct tttcacttat cacttatcac
     1141 tttttactca atcttatgac caaatggttt aaacgaaacg acggacaatt atttgaaact
     1201 gacgaaacaa gtgcagagtt caaaatgatg tccaaaagcg gcgaatttac gccgtgcgac
     1261 ccgggcggca atgaaagcga aactgactct caacaatccg ctgtggcaaa cagccgagcg
     1321 ccggagaatt ctggacaaag tggtgcagga aagcgcggtc gagctggaag gcgagataaa
     1381 gcggaaaatt ctgacgggaa ctaaatccgg cagaacctac cggcgcggcg cgataaccaa
     1441 agctgcaagt cagaaaaaca tagctttagg tttgcgccgc gcccgtggca atccgaaacg
     1501 ggttttcgcc ggaagcaggt ttcatcgagc cagcgcaccc ggcgaaagcc cggcaaacga
     1561 tactggctca ctggcgaact ctttgcgaag cgtttcagta aagcccttac acagcaaggt
     1621 ttttacaagc aagcgatatg ctgaaacttt agacaatccg caaaaattaa atcgcccttt
     1681 ttttagccaa acggcgcaaa aatttaaaaa gcgattcaaa gaacaaatta aaaatgct
//
LOCUS       DASWNE010000661         5598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_271063, whole genome shotgun sequence.
ACCESSION   DASWNE010000661 DASWNE010000000
VERSION     DASWNE010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5598
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271063"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VGB68_12900"
     CDS             <1..667
                     /locus_tag="VGB68_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050185.1"
                     /translation="NFVQSTAKDEDPLEKAKRNFRLFPADSASGSKAEETAAPDIINF
                     VENQLPPKSEAPTFERKKSTAVKFEKIASAKPKSTIEKTGSIVKKPSARREISNQFSP
                     AAFSRKTGGESAADINFEFPKRKTDSANVFPSKAETAAETPNFSSPWKKSRIDVQEYL
                     NPERDKKTDNQTDNPKKFAAAASPWIDLPDETAFEAADDIQINLSENGHLRFLEGEQA
                     GKI"
     gene            688..882
                     /locus_tag="VGB68_12905"
     CDS             688..882
                     /locus_tag="VGB68_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050186.1"
                     /translation="MIFGIGLIVLGVLGGLGVYFGLLAPVVQTPEVTGAIGKKAAGII
                     CLIAAVALIIAGALKLAGIW"
     gene            952..1680
                     /locus_tag="VGB68_12910"
     CDS             952..1680
                     /locus_tag="VGB68_12910"
                     /inference="COORDINATES: protein motif:HMM:NF039279.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050187.1"
                     /translation="MERVAFLIEETGERIACLLNPESVVMRRAAGVRPRRSIGGSLTG
                     AELADDVLLFTGGGTTEMFLNLLFDVALTDPAAKIEDVRSLTSPLWKLAENTSAALNY
                     GEPPRVRFVWGKSWNIPGVVASVSEHLDYFDQTGTPLRSWLRMRFLRAVEPLAETTAK
                     APPLPPLNPTLAAPAVPDREVKIHTFAAGDRLDQLVRQQYGFYGHPSLWRLIASYNSI
                     SDPLNIPPGTILQFPPLTDLEEKK"
     gene            1677..3278
                     /locus_tag="VGB68_12915"
     CDS             1677..3278
                     /locus_tag="VGB68_12915"
                     /inference="COORDINATES: protein motif:HMM:NF017739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEY0050188.1"
                     /translation="MNRQLKTLPQISVEIGGAALELSERAALAQIRVQQRLSLPALCE
                     LTFFDANGFLSNGDLFSPGADLRVTVAESDTPLFAGQITAVEHVYEPSNLRKIRLRGY
                     DKLHLLRKRQKVRAFVQMTLNDLIGELVKDFSLSVEASENSALRERIIQFEQTDFDLM
                     AELAERNGLYFALREDTLHVFTLEGTSDSVALKLGEDLLEARVEINADKICRSVSTTG
                     WNALRVENFSGQASSVRNGRSIGAEIDDANFGEPFERIITDENITDELEAVALSQAEL
                     DWRAAQEVTFWGVAEGNPQLQPGTPVEVEGIAQNLNGSYVLASVNHTIDTIKGFVSEI
                     STVLPKPVLKQKQSLATFGIVTGLDDPENYGRVRVKLPNYNDVETDWMQVVGMGAGLG
                     KGLIALPDIDDTVLVFFPRGALTHGIILGGLYGSIQQEDWDWGIEQSNVKRFRLQTAG
                     NQKISFDDVKKILRMENSDGSFVEMSPEKVSLHSQRDLEITAPGRGILIKAKTIDFER
                     AEEVVEPPNFQQVRAAEEREDADSE"
     gene            3262..3600
                     /locus_tag="VGB68_12920"
     CDS             3262..3600
                     /locus_tag="VGB68_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050189.1"
                     /translation="MLILNNEAKLVCKHEPGKVKNKPSQNFVTIEKKPVLVENDPEGR
                     TISGCPVIPPIGRSCVTTLTVRTGYSDWIRVGGKRICLDTITGFTDGTPPGTILYKVN
                     FAGQEFVSEK"
     gene            3604..4035
                     /locus_tag="VGB68_12925"
     CDS             3604..4035
                     /locus_tag="VGB68_12925"
                     /inference="COORDINATES: protein motif:HMM:NF016829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HEY0050190.1"
                     /translation="MKIKRYQAWKFIHPDLEANEEFAGLEIAPTGGIEMVAERDSVRQ
                     AILLLLSTIPGERVMRPDYGCDLYRLIFSPNDDTTAGLAIHYVKQAIERWEPRAKIEF
                     LDAFPNREDRARLDISLEYRLITTQALDALSFSVNLAGEEN"
     gene            4035..>5598
                     /locus_tag="VGB68_12930"
     CDS             4035..>5598
                     /locus_tag="VGB68_12930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HEY0050191.1"
                     /translation="MPLKTPNLDDRHFSQMVEESLRRARETCPQWTDLSPNDPGVVLV
                     ELFAVLTEAMIYRLNRVPEKAYVEFLRLIGVTLTPPTAAVVKLNFSLKKAPNQSLSIP
                     GGTRVTLSRAGGGGSSGEPPVFTTLQTVVIPKGKLEAEALAYNCEPVEAESVGKGTGM
                     PGLSVAVKRAPIVAQTSDDLEILVGVEASAEELKDRPRAKEHNGKNYRIWKEVENFSN
                     LDDKYVYMVDRVTGTITFAPAVQLRGQDGNLATGSAVSGEKPPEGREILVWYASGGGA
                     QGNVAANTLTVLKTQIAGVSVTNPAAATGGRDTETLQNALVRGPQELHSLHRAVTADD
                     FELLALRSSGAVTRAKAFTKAMVWSHAAPGTIEVLLVPNVPEQQRSGGAVTLETLQAQ
                     ETDEARQLIRRALDERRPLGTVCLVNWVHYKKVGVSAQAVIHRGADAESVKSRVLKRL
                     HQTINPLPSEDLNSDGWTFGEPLRASHIYDILLAERDVSYVENVRLHLDEVPENDITS
                     IAVDYFQPQTWYS"
BASE COUNT         1335 a         1507 c         1627 g         1129 t
ORIGIN      
        1 aaacttcgtt caatcgacgg cgaaagacga agacccgctg gagaaggcaa agcgaaattt
       61 ccggcttttt ccggctgact ccgcttccgg aagcaaagcg gaggagaccg ccgcgccgga
      121 cattataaat tttgtcgaaa atcagctgcc gccgaaatcc gaagctccga ctttcgagcg
      181 aaaaaaatcc acggcggtaa aatttgaaaa aatcgcgtcg gcaaagccga aaagcacaat
      241 cgaaaagacg gggtcaatcg tcaaaaaacc gtctgcgcgg cgggaaattt ccaatcaatt
      301 ttcgcccgca gcgttttccc gaaaaacggg cggcgaatca gccgccgata taaattttga
      361 gtttccgaaa cgaaaaacgg attcagcaaa cgtttttccg tcaaaagccg aaacggcagc
      421 ggaaacgccg aatttttctt cgccctggaa gaagagccga atcgacgttc aggaatatct
      481 taatccggaa cgcgataaaa aaaccgacaa ccaaaccgac aacccgaaaa agtttgccgc
      541 cgccgcatcg ccctggatag atttaccgga cgagacggca tttgaagcgg cggacgacat
      601 tcaaataaac ctttccgaaa acgggcatct tcgttttctg gaaggcgaac aagcgggaaa
      661 aatatagaaa tataaggaga taaataaatg atttttggaa taggtttaat tgtgctcggc
      721 gttctgggcg ggctcggagt ttatttcggt ctgttggctc cggttgttca gaccccggag
      781 gtgacaggcg caatcggcaa aaaagctgcc gggataatct gcctcatcgc ggcagttgct
      841 ctgattattg ccggagccct gaagctggcg ggcatttggt aaacgatttt aattttacaa
      901 accagagcga aaattaaaat ctgttttgag gaatacaaac agtcgtaatc tatggaacga
      961 gtcgcgtttt tgattgaaga aacgggcgag cgcatcgcct gcctgctgaa tcccgaaagc
     1021 gtcgtgatgc ggcgggcggc aggcgttcgc ccgcgccgct caatcggcgg cagcctgacc
     1081 ggcgcggagc ttgccgacga cgttctgctg tttaccggcg gcggaacgac cgagatgttt
     1141 ctcaatctgc ttttcgatgt cgctctgacc gacccggcgg cgaaaatcga agacgtgcgc
     1201 agcttgacgt cgccgctctg gaagctggcg gaaaatacga gcgcggcgct aaattacggc
     1261 gagccgccgc gcgttcgttt cgtctgggga aaaagctgga acattccggg cgtcgtggct
     1321 tccgtctccg aacatctgga ttatttcgac cagaccggca cgccgcttcg ctcgtggctg
     1381 cgaatgcgat ttttgcgggc ggtcgagccg ctggcggaaa cgacggcgaa agctccgccg
     1441 ctgccgcctc tcaacccgac gctcgccgcg ccggcagtgc ctgaccgcga ggtcaagatt
     1501 cacacctttg cggcgggcga caggctagac cagctcgttc gccagcaata cggattttac
     1561 ggtcatccgt cgctgtggcg tctgattgcc agctataaca gcatcagcga cccgttgaat
     1621 attccgccgg gaacgatttt acagtttccg cctttgacgg atttggaaga aaaaaaatga
     1681 accggcaatt aaaaacgctt ccgcagatta gcgtcgagat tggcggcgcg gcgctcgaat
     1741 tgagcgaaag agccgcgctc gcccagattc gcgtccagca gcgactctct ttgccggcgc
     1801 tctgcgaatt gacgtttttc gacgcgaacg ggtttttaag caatggcgat ttattttcgc
     1861 ccggcgcgga tttgcgcgtc acggtcgccg aatccgacac gccgcttttt gccgggcaaa
     1921 tcacggcggt cgagcacgtt tacgagccgt cgaacctgcg caaaatccgt ttgcgcggct
     1981 acgacaagct gcatcttttg cgcaagcggc aaaaggttcg agcctttgtc cagatgactt
     2041 taaacgattt gattggcgaa ctggtcaagg atttttcgct ctcggtcgaa gcgtcggaaa
     2101 acagcgccct gcgcgagcga atcattcaat tcgagcagac ggactttgat ttaatggcgg
     2161 agcttgccga aagaaacggc ttgtatttcg cgctgcgcga agacactctg cacgttttta
     2221 cgctcgaagg cacgagtgat tcggttgcgc tgaaattagg cgaagatttg cttgaagcgc
     2281 gcgtcgaaat caacgccgat aaaatctgcc gctcggtttc gacgaccggc tggaacgccc
     2341 tgcgcgtcga aaatttttcc ggtcaagcga gcagcgtgcg caacggcaga agcatcggcg
     2401 cggaaatcga cgacgctaat ttcggcgagc cgttcgaacg cattatcacg gacgaaaata
     2461 tcacggacga attggaagcc gtcgctcttt cgcaagccga gctggattgg cgcgccgcgc
     2521 aggaagtgac gttctggggc gtcgccgaag gcaacccgca gcttcagccg ggaacgccgg
     2581 tcgaggtcga aggcatcgcg cagaatttaa acggaagcta cgttctagct tcggtcaatc
     2641 acaccatcga cacgataaaa ggctttgtct ccgagatttc gaccgtttta ccgaaacctg
     2701 ttctgaagca aaaacaatcg ctggcgacct tcggcatcgt gaccggactc gacgacccgg
     2761 aaaattacgg gcgcgtgcgc gtgaaattgc cgaattacaa cgatgtcgaa accgattgga
     2821 tgcaggtcgt cgggatgggc gcggggcttg gcaaaggctt gattgcgctg cctgatatcg
     2881 acgacacggt gctggttttt tttccgcgcg gcgcgttgac gcacggcata atcctgggcg
     2941 gactttacgg ctcgattcag caggaagatt gggattgggg catcgagcag agcaacgtca
     3001 agcgctttcg cctgcaaacg gcgggcaatc agaaaatcag cttcgacgac gtgaaaaaga
     3061 ttttgcgaat ggaaaactcc gacggcagct ttgtcgaaat gtcgcccgaa aaagtttcgc
     3121 ttcattcgca gagagatttg gaaatcaccg ccccgggacg cggaattttg ataaaagcca
     3181 aaacgattga tttcgagcgc gccgaagaag tcgtggaacc gccgaatttt cagcaagtgc
     3241 gggctgccga ggagagagag gatgctgatt ctgaataacg aagccaagct cgtctgcaaa
     3301 cacgagccgg gcaaagtgaa gaacaaaccg tcgcagaatt ttgtgacgat tgaaaaaaag
     3361 ccggtgctgg tcgaaaacga cccggaaggc agaacgatca gcggctgtcc ggtcataccg
     3421 ccaatcggca gatcgtgcgt cacgacgctg acggttcgca ccggatattc ggactggata
     3481 cgcgtcggcg gcaagcgcat ttgtctggac acgataaccg gttttaccga cggaacgccg
     3541 ccgggaacca ttttgtataa ggtcaatttt gcgggacagg aatttgtctc cgaaaagtaa
     3601 agaatgaaaa tcaaacgcta ccaggcatgg aaatttattc accccgacct cgaagcgaac
     3661 gaggaatttg cgggcttgga gatagcgccg accggcggaa tcgaaatggt cgcggaaaga
     3721 gattcggtgc gccaggcgat tttgcttctg ctttcgacca ttccgggcga gcgcgtaatg
     3781 cgccccgatt acggctgcga tttatatcgt ctgatttttt cgccgaacga cgacacgacc
     3841 gccgggctgg cgattcacta tgtcaagcag gcgatcgaac gctgggagcc gcgcgccaaa
     3901 atcgaatttc tcgacgcgtt tccgaaccgg gaagaccgcg cccgtctcga catctcgctt
     3961 gaataccgtt tgattacgac gcaggcgctc gacgctttgt cattttcggt aaatctcgct
     4021 ggagaagaaa attaatgccg ctgaaaactc caaatttaga cgaccgccac ttcagccaga
     4081 tggttgaaga atccctgcgc cgcgcccgcg aaacctgccc gcagtggaca gacctttcgc
     4141 cgaatgaccc gggcgtcgtg ctggtcgagc ttttcgctgt tctgaccgaa gcgatgattt
     4201 atcgcctgaa ccgcgtcccg gaaaaggctt atgtcgaatt tctgcgtctt atcggcgtga
     4261 cgctgacgcc gccgaccgcc gcggtcgtca aattgaattt cagcctgaaa aaagcgccga
     4321 atcaatcgct gtcgattccg ggcggaaccc gcgtaacgct cagccgcgcg ggcggaggcg
     4381 gcagcagcgg cgagccgccg gtttttacca ctctgcaaac cgtcgtgatt ccgaaaggca
     4441 agctggaggc ggaggcgctg gcttataact gcgagccggt cgaagccgaa tcggtcggca
     4501 aaggcacggg aatgcccggg ctttcggtgg cggttaagcg agcgccgatt gtagctcaga
     4561 caagcgatga tttggagatt ctggtcggcg tcgaggcgag cgcggaagaa ctcaaagacc
     4621 gaccgcgggc gaaagagcac aacggcaaaa actatcgcat ctggaaggaa gtcgaaaatt
     4681 tcagcaatct tgacgacaaa tacgtttata tggtcgaccg cgtgacggga acgattacct
     4741 tcgcgcctgc tgtccagctt cgcgggcagg acggcaatct ggcaaccggc tcggcggttt
     4801 caggcgaaaa accgccggaa ggacgcgaga tactggtctg gtatgcgtcg ggcggcggcg
     4861 cgcagggaaa tgtcgcggcg aatacgctga ccgttctgaa aacgcaaatc gcgggcgttt
     4921 cggtgacgaa tccggcggcg gcgaccggcg ggcgcgacac ggaaacgctg caaaacgcgc
     4981 ttgttcgcgg accgcaggag cttcattcgc tgcaccgcgc cgtcaccgcc gacgattttg
     5041 aattgctcgc gctgcgtagt tccggcgcgg tcacgcgcgc caaagccttt accaaagcga
     5101 tggtctggag ccacgccgcg cccggcacga ttgaagttct tctcgtgccg aacgtgccgg
     5161 aacagcagcg ctcgggcggc gcggtcacgc tcgaaacttt gcaggcgcag gaaacggacg
     5221 aagcccgcca attaatacgg cgcgcgctcg acgagcgacg cccgctcggc acggtttgtc
     5281 tggtcaactg ggttcactac aaaaaagtcg gcgtctcggc gcaagccgtg attcatcgcg
     5341 gcgccgacgc cgaatcggtc aaatcgcgcg ttctcaaacg ccttcatcaa accatcaacc
     5401 cgcttccgtc cgaggatttg aattccgacg gctggacgtt cggcgagccg ctgcgcgctt
     5461 cgcatattta cgacatcctg ctggcggagc gcgatgtttc ctacgtcgaa aacgtgcgcc
     5521 tgcatctgga cgaagttccc gaaaacgata ttacgtcaat cgcggtcgat tattttcagc
     5581 cgcagacgtg gtattcgg
//
LOCUS       DASWNE010000662         2518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_373972, whole genome shotgun sequence.
ACCESSION   DASWNE010000662 DASWNE010000000
VERSION     DASWNE010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373972"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            417..2450
                     /locus_tag="VGB68_12935"
     CDS             417..2450
                     /locus_tag="VGB68_12935"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0050192.1"
                     /translation="MRPIKHVEKGLTVVAGGVFNAIRAINQYKPNPSFTPKWSDKPML
                     KSWQKSKPTLGFPRTTDSLCPNCVIEAREQILSGDREVATLINEKVGEIKAQIIERDG
                     QIWMIKDCPEHGHFEDLMAIDSAFLKHIEDLFPGRDIQSHNDEKLHNHGTSSIKYGRG
                     AVLTVDLTNRCNMMCDPCFMDANQVGFVHELSMEDVKEILDNAIQIKPRRQMSVQYSG
                     GEPTLSPYFLDAVRYARKVGYNSVQAATNGIEFAKSKEFCREAAEAGLRFVYLQFDGI
                     GNDANSHRQIGNLFDVKLRAINNLHEAGVDIILVTTLVNGINNDQVGSIIRFALDNPK
                     KISFIAFQPVSFTGRDEHITEQRRLQQRYTLSHLAQDVQKQVGITEPTRDWFPLSLMG
                     AFADFADVVHGPEAEWGQVSCGCHPNCGVGTAVMVNKETKEMAPVPQFLNIQGLVTDM
                     KHITDTNRGKWFSNILMGLALLKNYNPYGSPKSLTLGGILKKFDKSFGLSGKDYGKVG
                     PDRTIEDIEKRRQDPWNFLFIAGMWFQDLFNYDFRRTEMCIIPYGTQEGEISFCAYNT
                     GIGWRNIIEHMHQNATVAQWYKDHGRHEVFARGKEVELGDKTHSLVLNEVDLARPNKP
                     EMEGPKTAAEEMQMMKKLYQQMVLEKNSIKGEKLVQIGGIKREKKDKSENASA"
BASE COUNT          730 a          574 c          610 g          604 t
ORIGIN      
        1 atatttaaat aataactttt ccacatctgt gcaaaagtta agcaaaagtt taaatcaaaa
       61 ataaacttca tataaaaaat aagggcgagc gcggcatcta ttttgtgtca aaagtctcaa
      121 aatgaagtgt cggcagggaa tttgtgtcag agtgctacaa attgggctcg cgcggcttaa
      181 tctcaaaaag cctgtaagtc atttaaaaca aacgatttat tttagccatt ccaatggcat
      241 aattattgct tagatattta aatagttaaa gaggttctac ttcgctcatt tataataact
      301 taattaaatg caatttaatt gattagacaa gaacaggcat tgttgtatat acgcacgagt
      361 gaaatcgaac attttataat tttttacata aagctttctt ccggagaata gagaatatga
      421 gacctattaa acacgtcgaa aaaggattaa ccgtcgttgc aggcggagtt tttaatgcta
      481 ttcgcgccat taatcaatat aaaccgaatc cgtcatttac accgaaatgg tcggacaaac
      541 cgatgttaaa atcctggcaa aaatcaaaac cgacgctcgg ttttccgcgc acgaccgaca
      601 gcttgtgccc gaactgtgtc attgaggcac gcgagcagat tttaagcggc gaccgcgaag
      661 tggcaacgct cattaatgaa aaggtcggcg aaatcaaagc acagattatc gagcgcgacg
      721 gtcaaatctg gatgattaaa gattgcccgg agcacggaca ttttgaagat ttgatggcga
      781 tcgactcggc gtttttgaaa catatcgaag atttgtttcc cgggcgcgac attcaatcgc
      841 acaacgacga gaagctgcat aatcacggaa cttcgtcaat caaatacggt cgcggcgcgg
      901 ttttaaccgt cgatttgacg aatcgctgca atatgatgtg cgacccgtgt tttatggacg
      961 ccaaccaggt cggcttcgtt cacgagcttt cgatggaaga cgtcaaggaa attctcgaca
     1021 acgcgattca aatcaaaccg cgccgacaga tgtcggtgca gtattcgggc ggcgagccga
     1081 cgctttcccc gtattttctc gacgcggttc gatatgcgcg taaagtcggt tacaactcgg
     1141 tgcaggcggc gaccaacggc atcgagttcg ccaagtcgaa agaattctgc cgcgaggctg
     1201 ccgaagccgg acttcgcttc gtctatctgc aattcgacgg catcggcaac gacgccaact
     1261 cgcaccgtca aatcggtaat ttatttgacg tcaagcttcg cgccatcaac aatctgcacg
     1321 aagccggcgt cgatatcatt ctcgtgacga cattggtcaa cggtattaac aacgaccagg
     1381 tcggctcgat tattcgcttc gcgctggata atccgaaaaa gatttcgttt atcgctttcc
     1441 agccggtttc ctttaccgga cgtgacgagc atattacgga gcagcgtcgc ctgcagcagc
     1501 gctatacgct ttcgcatctg gcgcaggacg tgcagaaaca ggtcggaatc accgagccga
     1561 cgcgcgattg gtttccgctt tcgttaatgg gcgcgtttgc cgatttcgcc gacgtggttc
     1621 acggtcccga agccgagtgg ggacaggttt cctgcggctg tcacccgaat tgcggcgtcg
     1681 gaaccgccgt gatggtcaac aaggaaacga aggaaatggc tccggttccg caatttctga
     1741 acattcaggg attggtcacg gatatgaagc acatcaccga tacgaatcgc ggaaaatggt
     1801 tctcgaatat cctgatgggc ttggcgctgt tgaaaaacta caacccgtac ggctcgccga
     1861 aaagtctgac gctcggcgga attttgaaga aattcgataa atcgttcggc ttgagcggaa
     1921 aggattacgg caaagtcggt cccgaccgca cgattgaaga catcgaaaaa cgtcgtcaag
     1981 acccgtggaa ctttcttttc atcgcgggaa tgtggtttca ggatttgttc aactacgatt
     2041 ttcgacgcac ggaaatgtgc atcattccct acggcacgca ggaaggcgaa atcagcttct
     2101 gcgcttataa cacgggaatc ggctggagaa atatcatcga gcatatgcac cagaacgcaa
     2161 ccgtcgctca atggtataaa gatcacggtc gtcacgaagt tttcgctcgc ggcaaagaag
     2221 tcgagctcgg cgacaagacg cacagtctcg tcctgaacga agtcgatctg gcgcgtccga
     2281 acaaaccgga aatggaagga ccgaaaaccg ctgccgaaga aatgcagatg atgaaaaaac
     2341 tctaccaaca aatggtttta gagaaaaaca gcatcaaagg tgagaagtta gtgcagatcg
     2401 gtggaatcaa acgcgagaaa aaagacaaat cggaaaacgc ttcggcatag tttttcgtta
     2461 aattaaaaaa gcgggacaga ttaataaaat tttattaatc tgtcccgctt ttttaatt
//
LOCUS       DASWNE010000663         2643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_619471, whole genome shotgun sequence.
ACCESSION   DASWNE010000663 DASWNE010000000
VERSION     DASWNE010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619471"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VGB68_12940"
     CDS             complement(<1..186)
                     /locus_tag="VGB68_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050193.1"
                     /translation="MKNEHILEILDEKAFADFDEAELGFIKAHAAQCVDCFQAFEAAR
                     ISAVLLRTHAAESFEPSP"
     gene            complement(188..799)
                     /locus_tag="VGB68_12945"
     CDS             complement(188..799)
                     /locus_tag="VGB68_12945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY0050194.1"
                     /translation="MKDYAELSDLELARASGAGDENAFAEIVRRYTPRVFRFVSRFFR
                     RRSVVEEVAQDVFLRIFTQLKSYEGRGSFEGWLTRITVNTCINQLRSAKRETELTVSA
                     LSEEENDWLEQKMSDAAAVRHQSDEEKLVAADLVNRVLQTMSADDRLVLTLIDGEGHS
                     IRDICEMTGWTESKVKIQAFRARRRMREAVENLMRKTHRQQKA"
     gene            1278..2621
                     /locus_tag="VGB68_12950"
     CDS             1278..2621
                     /locus_tag="VGB68_12950"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0050195.1"
                     /translation="MHFFNSFRRSGFFGVFILFLAALVFSAACGGSSGQGNRRGGGNG
                     NGNSQPIPDAGPVVTVTASKAVGREVPAFIQSTGTLVANETSDVAPKVAGKVVNVSAN
                     VGQFVGQGDVIAKIDDRDARLRLAEAQAGVTQAIAGVRQAEARLGLSPNGTFNASTIP
                     EVQAANASYEQALAELRQAEANERRYRDLAETGDVSIQNYEQYRTARDTARARVNNAR
                     QQQEAARNAARQNNQAIRAAQAGVEAARTQVGTAQQAVVDTVVRAPFAGFVSNRPVAV
                     GEYVSSASIIATILRANPIKLQMQVAEADVPYVTIGKAVSLEVDAYKDRKFGGTVTAV
                     NPAIDPSSRSAIIEASVENGDNALRSGMFATARIVREGGNAGVFVPRTALVNDQTTQS
                     YRVFVIQENVAKLRVVQLGTEEGDMVQILTGVNADETIAVSNLDQLYEGARVEIQ"
BASE COUNT          586 a          743 c          719 g          595 t
ORIGIN      
        1 cggcgacggc tcgaagcttt cagccgcgtg cgtgcgcaat aaaacggcgg aaatccgcgc
       61 ggcttcaaag gcttgaaaac aatccacgca ctgcgcggcg tgcgctttga taaagccgag
      121 ctccgcttcg tcaaaatcgg cgaacgcttt ttcgtccaaa atttctaaaa tatgttcgtt
      181 tttcatttta agccttctgc tgtctatgcg ttttccgcat taaattctcg accgcctcgc
      241 gcatacgccg ccgcgctctg aaagcctgaa tcttgacttt cgactcggtc cagccggtca
      301 tctcgcaaat gtctcgaatc gaatgtcctt cgccgtcgat taacgttaaa accagccggt
      361 cgtccgccga catcgtttgc aaaacgcgat tgaccaaatc ggcggcaacc agtttttcct
      421 cgtccgattg atgccgcaca gccgccgcgt ccgacatttt ttgttcgagc cagtcgtttt
      481 cttcctcgct gagggcggaa acggtcaatt cggtttcgcg cttggcgctg cgcagctgat
      541 tgatgcaggt gttgaccgtg atgcgcgtca gccagccttc aaaggagccg cgcccttcgt
      601 aacttttcaa ttgcgtaaaa atccgcagaa aaacgtcctg ggcgacttct tcgaccacgc
      661 ttcgccggcg aaaaaagcgg ctgacgaagc ggaaaactct cggcgtataa cggcgcacga
      721 tttccgcaaa ggcgttttcg tcgcccgcgc cgctcgcccg cgccagttcc aaatccgaca
      781 gttccgcgta atctttcaag caatgctccc ttgctttatc actttcgata aatttttttc
      841 gaattgtatc tactagaatg atattcacaa gcgccgtcga aagaaagtct gagaatcgtt
      901 ttaaaacctg cccttgctcg cctattttga taagacacga aatcggcgga aaaagttaca
      961 tccgattttt tgtttgattt cagaacggtt ttttctcgcg tttccggcgc tgaaagcatt
     1021 tgcaaacctc gcaaacgctc ccgcatctca cgccgatgtg cttgtgcggg aaacgcgaga
     1081 gcaagctaag cggcagctta aaagcaaagt tttgtaaaga agccggctca attgtaacca
     1141 tttaaagttt acgactgtct ataaaagtta cggcgatttt ttaaaacgcg ggcgggcgat
     1201 aatttaaaac tcaattcaga aagctctcgc cgcgggattt tttagcaaat cgtttgtttt
     1261 tgcggtaaaa caattgtatg cactttttta attctttccg ccggagcggt tttttcggcg
     1321 tctttatttt atttctggcg gctctggttt tttcagccgc ctgcggcggc agctccgggc
     1381 agggcaatcg ccggggcggc ggcaacggta acggcaacag ccagccgata ccggacgctg
     1441 gtccggtcgt tacggtgacg gcttcgaaag ccgtcgggcg cgaagttccg gcttttattc
     1501 aatcgaccgg aactctggtg gctaacgaaa cctctgacgt tgcgccgaaa gtggcgggaa
     1561 aagttgtcaa cgtgtcggcg aatgtcggtc agtttgtcgg gcagggcgac gtcattgcca
     1621 aaatcgacga ccgcgacgcg cggctgcgtc tggcagaagc gcaagccgga gtgacgcagg
     1681 caatcgccgg cgtccggcag gctgaagcgc ggctcggact ctcgccgaac ggaactttta
     1741 acgcttcgac gattcccgaa gttcaggcgg caaacgccag ttacgagcag gcgctcgccg
     1801 aattgcggca ggctgaagcc aacgaaagac gctaccgcga tttagcggaa acgggcgacg
     1861 tttccatcca gaattacgag caatatcgca ccgcgcgcga cacggcgcgc gcccgcgtca
     1921 acaacgcccg ccaacagcag gaagcggcgc gcaacgccgc tcgccagaat aatcaggcga
     1981 ttagagccgc gcaagccggg gtcgaagccg cgcgaacgca ggtcggaacg gcgcagcaag
     2041 cggttgtcga tacggttgtg cgcgctcctt tcgccggttt tgtcagcaat cgcccggtcg
     2101 ccgtcggcga atatgtttcg tccgcgtcaa tcatcgcgac gattttgcgc gccaatccga
     2161 ttaagttaca gatgcaggtc gccgaagccg acgtgccgta cgtgaccatc ggcaaagccg
     2221 tctcgctcga agtcgatgcc tacaaagatc gcaaattcgg cgggacggtg acggcggtca
     2281 atccggcgat tgacccgtcc tcgcgctcgg caatcatcga agcgtccgtt gaaaacggcg
     2341 acaacgccct gcgctccgga atgtttgcga ccgcgcgcat cgtccgcgaa ggcggcaacg
     2401 cgggcgtttt cgtgccgcgc accgcgctcg tcaacgacca gacgacgcag tcttatcgcg
     2461 ttttcgtcat tcaggaaaac gtcgccaaat tgcgcgtcgt ccaattgggc accgaagaag
     2521 gcgatatggt gcagatttta accggcgtca atgccgacga gacgattgcc gtcagcaatt
     2581 tagaccagct ttacgaaggc gcgcgcgtgg agattcaata ggattcagga ttcaggattc
     2641 agg
//
LOCUS       DASWNE010000664         5177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_305859, whole genome shotgun sequence.
ACCESSION   DASWNE010000664 DASWNE010000000
VERSION     DASWNE010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5177
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305859"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VGB68_12955"
     CDS             complement(<1..419)
                     /locus_tag="VGB68_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050196.1"
                     /translation="MKKSIYKNFTALLSLLLIFSSLAPVFAQKKRGGNTSAPVKKQTA
                     KVPAAEGGEKESCDVWTGTIEITQRRAETRTKVTNKGEHVSDQHYTGKAESKSSYDYK
                     GIFNVSNSTGGANTYEDGSKYLSLKGTVSAEALRVME"
     gene            complement(599..931)
                     /locus_tag="VGB68_12960"
     CDS             complement(599..931)
                     /locus_tag="VGB68_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050197.1"
                     /translation="MKNIEFIKTFSLGETVGIGKPKDVVEIFNGSRRRMVQVNLRSGE
                     ILSKHKANEPITVFCLAGNGTFKAGPLLEDEQKLVAGTLITLEGGVEHEVIAEPEISL
                     LVTKFKAE"
     gene            1113..1811
                     /locus_tag="VGB68_12965"
     CDS             1113..1811
                     /locus_tag="VGB68_12965"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY0050198.1"
                     /translation="MKTDKIEVLKQTELFRDLDDVILRVLAGHAVEKSLERNEILFLA
                     GDEAKGLYVVAAGAVRAFRTGADGREQVIHVERAVTTIAEVPVFDNGTYPSTVSAEEP
                     TLVYFLDKQEVFRLSLNHPEIALAATRLLARRLRKCAELVESLSLREVGQRLARLLLE
                     EAEASGLAVVNGVRFKQNLTHNQLAARIGTVREVITRVLYRLQIQGLIIVEGKDVVVP
                     DLELLANYADSEKR"
     gene            complement(1988..3949)
                     /locus_tag="VGB68_12970"
     CDS             complement(1988..3949)
                     /locus_tag="VGB68_12970"
                     /inference="COORDINATES: protein motif:HMM:NF020305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HEY0050199.1"
                     /translation="MRAASAERSDGRAAAAFAFTSWRRRCSKKINGELEDIKLKDGIL
                     VHESTKIDLETFTRQNGEKPVEPFMLIVARDTDHAETLMRVIKADDFFEGRYKDKVIT
                     VHSNQRGEESDETVARLLAIENPQESTEIVIHVNMLKEGWDVTNLYTIVPLRAANSRT
                     LVEQSIGRGLRLPFGKRVGVNAVDRLTIVAHDKFSEIIDEANRPDSVLKVVPVYIGKD
                     IAVEEKKNVTAAPALEAALFGEPAPETEMTGTQTANGENSTLPVQRKIAFHKPIEREI
                     AQTTLEVIRRFEHKPSSNDLRKPEVRAEILREVKESLPLRAQADFTEIINPPKFEEIV
                     EKVTEKFIEMTIDIPKIVVVPKGETIWTYRDFDLDTSNLNIPPTSHSILLQSLQTGKQ
                     ERLADRADNQRELLPENYLVKRLIDFDDISYDEHADLLYRLSGQMVSHLRSYMPDEAA
                     VCDTVQFESERLAKLIYAQMLAHQSQNVADFEVTVTKGFTTLRNNNYTIQAREQVRGF
                     RAAVESRQNIRSMHFGGFEKCLYQTQKFDSDSERRFSIVLENDAAVLKWFKPAPGRFR
                     IFFHQDKQYAPDFVVETQSEKFLCEVKREDDLEDAEVQSKKSAAEKWCEHASRHESEN
                     GGKPWRYVLVAHNSIAAASTLAGIVSRSI"
     gene            complement(3846..5174)
                     /locus_tag="VGB68_12975"
                     /pseudo
     CDS             complement(3846..5174)
                     /locus_tag="VGB68_12975"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
BASE COUNT         1179 a         1300 c         1189 g         1509 t
ORIGIN      
        1 tcttccatta cgcgcagggc ttcggcgctg actgtcccct tgagcgacag gtattttgag
       61 ccgtcctcgt aagtgttcgc gccgccggtc gaattggaga cattgaaaat ccctttatag
      121 tcgtacgacg attttgactc cgcctttccc gtgtaatgct ggtcgctgac gtgttcgcct
      181 ttattggtta ccttcgtacg agtttcggct cgacgctgcg tgatttcaat cgtcccggtc
      241 caaacgtcgc agctttcctt ttcgccgcct tcggcagccg gaactttagc cgtttgtttt
      301 ttaaccggcg ctgatgtatt gccgccgcgc tttttctggg caaaaaccgg agccagcgaa
      361 gaaaaaatga gcaataaact caacagagcc gtaaaatttt tataaatgct ttttttcata
      421 actttataaa tgttttctct attgtaaagc gtgaaacggg cgtaaaaagt atcgtgaaac
      481 tttcagcccg gctcgaatcc gcgtaaaacc gatttatcaa atcatcgatg tttgctcgcg
      541 tttgaatgtc ttttttcata tttttctatt caaatacttt tcccgcgcag gcagcgaatc
      601 attcagcttt gaattttgta accaacaaac ttatttccgg ttcggcaatg acttcgtgtt
      661 cgacgccgcc ttctaaagtt atcaatgttc cggcaacaag tttttgctcg tcttccagaa
      721 gaggtccggc tttaaacgtt ccgtttccgg ctaaacagaa aacggttatc ggctcatttg
      781 ccttatgttt cgacaagatt tcgccgcttc tcagattgac ctgaaccatt ctgcggcgcg
      841 agccgttaaa gatttctacg acgtccttcg gctttccgat tccgaccgtt tcgcctaacg
      901 aaaaagtttt tataaattcg atgtttttca tattattttt acctctgatt aattaccgga
      961 atttctttaa gatttattgt caaactctct tttgacgatt cgattgtcgc gccgtttgcc
     1021 gctctaaaaa gtgatttaag tcaccttttc aaactttttt gttaataagt aactatttgg
     1081 aaaatgaccg gaaagaccgt taaacttttc gggtgaagac agacaagatt gaagttttga
     1141 aacagacgga gcttttccgt gacctggacg acgtaatcct tcgcgtttta gccggtcacg
     1201 ccgtggaaaa gtcgttagag cgcaacgaaa ttctttttct ggcgggcgat gaagcgaaag
     1261 gcttatacgt ggttgcggcg ggcgcggtgc gtgcttttag aaccggcgcg gacgggcgcg
     1321 aacaggtgat tcacgtcgag cgcgcagtca caacaatcgc tgaggttccg gtattcgaca
     1381 acggaactta tccgtcgacg gtttcggcgg aagaaccgac attagtttat tttctggata
     1441 aacaggaagt tttccggctc agtttaaatc acccggaaat tgcgcttgcc gcgacgagat
     1501 tgctcgcaag acgtctccgg aaatgcgccg agttagtcga atcgctcagt ttgcgcgagg
     1561 tagggcagcg cctcgctcgc ttgcttttag aagaagccga agccagcggg ctcgcggtcg
     1621 taaatggagt tcgtttcaaa caaaacctga ctcacaatca gctcgccgcc cggattggaa
     1681 cggtgcgtga agtgattacc cgtgtccttt accgtctcca aatccagggt ttaattattg
     1741 tcgagggaaa agacgtcgtc gttccggatt tggaattgct ggcgaattat gccgattcgg
     1801 agaagcggta attcctcttt atagtaaata aatctttcga tttccggtaa aggctaaagc
     1861 ttgaaacttc cccggctggg attttcagca ggttttatcg gtttagagct ttttgccgaa
     1921 aaatctattt taaatctatc ttatcgattt tatttacaag ggtttgcgaa agatataatt
     1981 cccgatttta tatgctgcgc gaaacgattc ccgccagcgt cgaagccgct gcaatcgaat
     2041 tgtgcgcaac cagaacatac cgccagggct tgccgccgtt ttcgctttcg tgccggctgg
     2101 cgtgctcgca ccatttttcc gcggcggatt ttttgctctg cacttcggcg tcttccaaat
     2161 cgtcttcgcg ctttacttcg cacaaaaatt tttcgctctg cgtttcaacc acaaaatccg
     2221 gtgcgtattg tttatcctga tgaaaaaaaa ttcgaaaccg ccccggcgcc ggtttgaacc
     2281 atttcaaaac cgcggcgtca ttttccagaa ctatcgaaaa acgccgttcc gaatcggagt
     2341 cgaatttttg cgtctggtac agacattttt caaagccgcc gaaatgcata ctgcgaatgt
     2401 tttgacggct ttcgaccgcc gcgcggaagc cgcgcacctg ctcccgcgcc tgaatcgtat
     2461 aattattgtt tctgagcgtc gtaaagcctt tcgtcaccgt gacttcgaaa tcggcaacat
     2521 tctgtgactg atgcgcgagc atctgggcgt aaatcagctt ggcaagacgc tcgctttcaa
     2581 actgcaccgt gtcgcaaaca gccgcttcgt ccggcatata cgaacgaaga tggctgacca
     2641 tttgcccgct cagccgataa agcaaatcag cgtgctcgtc gtatgaaata tcatcgaaat
     2701 caatcaatct tttaaccagg taattttccg gcagcagctc gcgctgattg tctgcgcggt
     2761 cggcgagtct ttcctgtttg ccggtttgaa gactttgcag cagaatgctg tgcgaggtcg
     2821 gcggaatatt cagattgctc gtatccaaat cgaaatcgcg gtaagtccag attgtttcgc
     2881 ctttcggaac gacgacgatt ttcggaatat ctatcgtcat ttcgataaat ttttccgtca
     2941 ccttttcgac aatttcctca aacttcggcg gattgattat ttcggtaaaa tcagcctgcg
     3001 cccgcagcgg cagagattct ttgacctcgc gaaggatttc ggctcgaact tcgggttttc
     3061 ttaaatcatt cgagctcggt ttatgctcga agcggcgaat gacttctaaa gtcgtctgcg
     3121 cgatttcgcg ttcaatcggt ttgtgaaagg cgattttccg ctgaaccggc aaggtcgaat
     3181 tttcgccgtt tgccgtttgc gttcccgtca tttcggtctc cggcgccggt tcgccgaaaa
     3241 gcgcggcttc gagcgcgggc gcagccgtaa catttttttt ctcttcgacg gcgatgtctt
     3301 tcccgatata aaccggcacg acctttaaaa cggaatccgg acgattggct tcgtcgataa
     3361 tttcggaaaa cttgtcgtgc gcgacaatcg tcaggcggtc gaccgcattg acgccgactc
     3421 tttttccgaa cggaagacgc aagccgcgcc cgattgattg ctcgaccagc gtgcgcgaat
     3481 ttgccgcgcg aagcggaaca atcgtataaa gattcgttac gtcccagcct tccttgagca
     3541 tattgacgtg aataacgatt tcggtcgatt cctgcggatt ttcaatcgcc agcagacggg
     3601 cgaccgtttc gtccgattct tcgccgcgct gatttgaatg aaccgtaata actttatcct
     3661 tataacgccc ttcgaaaaag tcgtccgcct ttatgacccg catcaaagtt tcggcgtggt
     3721 cggtatcgcg ggcgactatc agcataaaag gctcgaccgg cttttcgccg ttttgtctgg
     3781 tgaaagtttc gaggtcaatc ttggtgcttt cgtgaacgag tatgccgtct ttaagcttta
     3841 tatcttcaag ctcgccattt atcttttttg agcagcgacg gcgccagctc gtaaaagcga
     3901 aagccgccgc cgcccttcca tccgaccgct ctgctgatgc cgcccgcatc ttcgccgtca
     3961 atcagttttt tcagacgcgg aataatgtgc gtgtagcaat gctcgcccaa ttcgaccatt
     4021 atccagcgcc gacgcatttt atgcgcgacc gcgccggtcg ttcccgaacc gccgaaagaa
     4081 tccaaaacca aatcgccttc gttcgtataa atttcaaaaa ctctttttat cagagcttcg
     4141 ggtttttttc catttttgaa ttcaacgccg ccttccgacg cgattccgtt ccaggaaata
     4201 tccgtccaga catcggtgat aatttcggcg ggaactttcg ttccgtcgac ttcgacgatt
     4261 cgatgttcgt aaaacgcaat ctgccttccg ttgacaatgt aaaaatcctt gacgtcttcg
     4321 ttcggatgaa cgaaaaccac gtctctctga ttttgagatt tttgaatggt cgaaagtcgt
     4381 ttgatttttg cgccgccgcc gatagccacc agctgaaaaa ctctcccggc attttccatc
     4441 gcataatccg ctatctcggc gtcgaagctt tctttcaatg cttttttggc ttctctcgca
     4501 ttaggaaatc ccagtttctc ggcgacgact tccgaaattc cgcgccagcg ccagttttca
     4561 taaggctcgt ttctgttttc cagcactttc gagtaaccgg cgtcgtaacc tttttccaca
     4621 tataatcttt tcgtcgttgc agatgattta ttttttgcaa atacaagaag ataattggcg
     4681 gtagaaaaaa gtttcgaaga cgtggcttta aaaccggaag ggtcgttggt cgttaaagta
     4741 atcgtgttga tatagtttga ccgcccgaaa acttcgtcca gaacgacttt tcagtaagcc
     4801 atttcttcat cgtctaaatg aacgattaaa aagccttcgt cggctaataa ctttcttaaa
     4861 atctcaaatc tgctcttcat ccggttgagc cattcggaat gttccagccc gtcgtcgtaa
     4921 taatcggttg cggcttgaat gttaaacggc gggtcgatgt aaacgcattt caccttgcct
     4981 gcaaattcct gctccagcgc tttaagcccg agcagattgt cgcctttgat taagacattg
     5041 tcgaaaatgt ctttgtccga gatgcgatgc ggagcgtgat acgagaggtt tttatcttcc
     5101 agcagaatgc gcggctcaag acgcgggcgc tcttcttttc caatccacat cagttcgagt
     5161 ttttgcttct tcataaa
//
LOCUS       DASWNE010000665         2597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_305860, whole genome shotgun sequence.
ACCESSION   DASWNE010000665 DASWNE010000000
VERSION     DASWNE010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305860"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..556)
                     /locus_tag="VGB68_12980"
     CDS             complement(77..556)
                     /locus_tag="VGB68_12980"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY0050200.1"
                     /translation="MPMRIGDARPSFEGATGWLNELQETADDYVNGQPTLVYFWATSC
                     GICKENMPKLQELKAKYKDRGLRTVAIHMPRYEADTDLENVRATIQQHCIDDICAVDN
                     EHKLKEAFLNEQGWVPVYYLFDADGKLKSRAAGEFGVNVLNGALEKMFPAQASEANA"
     gene            complement(563..688)
                     /locus_tag="VGB68_12985"
     CDS             complement(563..688)
                     /locus_tag="VGB68_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050201.1"
                     /translation="MANERDEPTTNENNGENQSSERPILTNLLPEKGILYFNITR"
     gene            complement(775..1362)
                     /locus_tag="VGB68_12990"
     CDS             complement(775..1362)
                     /locus_tag="VGB68_12990"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019258276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY0050202.1"
                     /translation="MATATGTATENTATPTLAKVGQPAPDFNMPSTKNMETLAENVKL
                     SDYKGKWLILLFYPLDFTFVCPTELTAFSDRIDELQGIGAEVIGVSTDSVHSHRAWIK
                     TPRDQNGVADLQYPLASDTGGRLAAKYNILVEEDGIALRGLFIINPEGILQYAVVHDL
                     NIGRSVDETLRVLQGLQTGGLCAADWTPGQENLKV"
     gene            complement(1390..1794)
                     /locus_tag="VGB68_12995"
     CDS             complement(1390..1794)
                     /locus_tag="VGB68_12995"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEY0050203.1"
                     /translation="MSVSVNIKDLGLTRQREVVLQVIRDAEEHLTANEVFDKAKQLLP
                     SISFATVYNSLRYLKDAGHIAEISFGNGASRFDSMTSRHDHAICTKCGKLVDMELDLP
                     IELVNLAAEYSKFKPESLELTLRGLCPNCSNT"
BASE COUNT          636 a          621 c          580 g          760 t
ORIGIN      
        1 caaaatgtaa atcctgttta cattttgcgc tccgtatctt gaatttatag acgtttaccg
       61 cctttttagt aaaaaactaa gcgtttgctt cgctggcttg cgcgggaaac attttttcga
      121 gcgcgccgtt caaaacgttg acgccgaatt cgcccgccgc gcgcgatttc agttttccgt
      181 ccgcgtcgaa cagataataa accggaaccc agccctgttc attcaaaaac gcttccttta
      241 atttgtgctc gttatcgacg gcgcaaatgt cgtcgataca gtgctgctga atcgtcgctc
      301 tgacattttc caaatcagtg tcggcttcgt agcgcggcat atgaatcgcc accgtgcgca
      361 agcccctgtc tttatatttt gcttttaatt cctgtaattt cggcatattt tccttgcaga
      421 ttccgcagct cgtcgcccag aaataaacga gcgtcggctg accgttgaca taatcgtcgg
      481 cggtttcctg caattcgttg agccatccgg tcgcgccttc aaacgacggg cgcgcgtctc
      541 caattctcat cggcatatta aatcacctcg taatattaaa atataaaata cctttttccg
      601 gcagaagatt ggttaaaatc gggcgttcgc tgctctggtt ttcgccgttg ttttcatttg
      661 tggtcggttc gtcccgctcg ttcgccataa attgtttttc tcctttgtgc gtaaaaaaag
      721 taagagacga aagaatatcg ctgttctctc gcctctcgta acttgttcac aaagttatac
      781 tttcagattt tcctgtcccg gcgtccagtc agcggcgcac aatccgccgg tttgcaagcc
      841 ctgcagaacg cgcagcgttt cgtcgaccga gcgtccgatg tttaaatcgt gaacgacagc
      901 gtattgcaga atgccttccg ggttgatgat gaaaagtccg cgcagcgcga tgccgtcttc
      961 ttcaaccaga atgttgtatt tcgccgctag cctgccgccc gtgtccgaag cgagcgggta
     1021 ttgcaaatcg gcaacgccgt tctggtcgcg cggagtttta atccaagcgc ggtgagaatg
     1081 aaccgaatcg gtcgaaacgc cgataacttc cgcgccgatg ccctgcagct cgtcgatgcg
     1141 gtcggaaaaa gcggtcaatt ccgtcgggca gacgaatgta aaatcgagcg ggtaaaacaa
     1201 taaaatcaac catttgcctt tgtaatcgga aagcttgacg ttttccgcca gcgtttccat
     1261 atttttggtc gaaggcatat taaaatccgg tgccggttgt ccgactttcg ccagtgtcgg
     1321 cgtcgcggta ttttccgtag ctgttcctgt agctgtcgcc ataaaaaaaa tctccttgat
     1381 ttgttaaaat taagtattac tgcaattagg gcacaatccg cgcagagtaa gctccaggga
     1441 ttcgggtttg aattttgaat attccgccgc taaattgacc agctcaatcg gcaaatcgag
     1501 ctccatatcg accagctttc cgcatttggt gcagatggcg tggtcgtgcc gggaagtcat
     1561 cgaatcaaaa cgactcgcgc cgttgccgaa actgatttcc gcgatgtgac cggcgtcttt
     1621 cagataacgc agcgaattgt aaaccgtagc aaaacttatg ctcggcaaaa gctgtttggc
     1681 tttatcgaaa acctcattcg ccgtcagatg ctcttcggcg tcacggataa cctgcagaac
     1741 aacttcgcgc tgcctggtca aacctaaatc ttttatattt actgatacgc tcatttatgt
     1801 cctaagttag atttagaata attctaattc taaaaaatgt caagctgatt ttacgggtga
     1861 gaatttaatg aaagtgtgtt actggaacga tgagttggca caaatgccgg cttatgtttc
     1921 taaaaataat gcgctatctt gtgcttccaa taaataatca cttaattttg attgttacgc
     1981 gggaatttta tctgctttta catatagccg agttcagttc tggagttaaa ttgtatgaag
     2041 gtagttttat tgtcctcttc tttttactcg tcttttaatg tcgatatcta tttatcactt
     2101 ataattctct cattactatc gccaaataat ctcatttgat ttaaaaactc aaacaaatgt
     2161 tttacccaac aaagtcttct atgttttatg ccttttttta ttagaagaat attgcgaatc
     2221 ctaatgtgaa aatttccgtg gtcgtgtagc cgcggaaagg tattttttcg gctcgcgccc
     2281 cggcgccgaa aagaaacgaa agaaaaatca gcgcggcggc gcgaaataag ccgattaaaa
     2341 ttctttttgc cttaaattgc cacgctgaat catctgccga aagcgggcag ccccgttatc
     2401 gacgccggct ccaatgcgct ggcggtcgat gaatcaggca atccgctgct taccgaccag
     2461 cgcggacttg tccgtatctt cggaagcgcg gttgagatcg gcgcggttga agtatcgaat
     2521 taataaatga catctgccgg agatttcata atttccggga attgaaatcg gcattttcgg
     2581 gaattgcatt ctgctcc
//
LOCUS       DASWNE010000666         7308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_271255, whole genome shotgun sequence.
ACCESSION   DASWNE010000666 DASWNE010000000
VERSION     DASWNE010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7308
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271255"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1200)
                     /locus_tag="VGB68_13000"
     CDS             complement(<1..1200)
                     /locus_tag="VGB68_13000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0050204.1"
                     /translation="MKSIKFIIFAATACFLSIFIISCGGSSAKNNNANSNTNTLPTVV
                     EATTATAIVQNVPTYFEATGSLASDAQTDVAPTVGGKIVEVNFDIGSYVQKGAVLVRL
                     DDRDARIRLEQAQAQVAQAQSNVRQAQAQVEQARASVRQTQSRLGITEGSNFDIETFS
                     QVRATKAQLELAEKELGRAERLLESGDIARTIYDQRKAQRDQLRAQLDESRSTAAVAV
                     SAIRTAMTQVSSAQAAVGTARSAEEAARTQISSAQKAIGDAVVYSPISGYVSERVADV
                     GEFISPNTPSSKVATIVRTSVLRMRIDVPEQNVGQVKAGQGISLQTSAYPDRNFAGTV
                     VRISPSVNATSRTLTVEAEVENGEGLLKPGQFATVRIAQGAPKQSVMIPTVAVKTEGD
                     TNRVYDIK"
     gene            complement(1235..1882)
                     /locus_tag="VGB68_13005"
     CDS             complement(1235..1882)
                     /locus_tag="VGB68_13005"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0050205.1"
                     /translation="MIKELDTTETTHGRMSGDERRSQILQVAIKLFSQNGFRGTTTKE
                     IARAAGVSEAMVFRHFATKDELYHAILDYKACEGRAVNPMEAMCESLAAKDDEQVFYQ
                     MILNALNKHEGDPQFMRLLFHSALEGHELAEIFIEENIVPIYEFMSAYIAQRQKDGVI
                     RQNINPRVIVRAIVGMMIHHALNNSLWDRKRRLLDITNEEAAKNFTEILLQGIKN"
     gene            2103..2435
                     /locus_tag="VGB68_13010"
     CDS             2103..2435
                     /locus_tag="VGB68_13010"
                     /inference="COORDINATES: protein motif:HMM:NF039524.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050206.1"
                     /translation="MAEIEETNNEQGLVKPKIYNGEDKAENDYVGEFKVQAQEYGQRL
                     NDAALKAKDYASEKFDLASEKFRELQAKDPKELIEDAKEYARQKPAQTILISAAVGLV
                     LGLLLKRR"
     gene            complement(2613..2963)
                     /locus_tag="VGB68_13015"
     CDS             complement(2613..2963)
                     /locus_tag="VGB68_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050207.1"
                     /translation="MAGKKFAVSDSVERMCSACDTETNHSVLTVTKQGQISKLTCDVC
                     AMESTYSRDVKTSVTVGRTKSGSPYDRTRKYRKGQAMVHDVFGQGEVMEVIEPQKIDV
                     LFGDRVRRLIHAQV"
     gene            complement(2982..4187)
                     /locus_tag="VGB68_13020"
     CDS             complement(2982..4187)
                     /locus_tag="VGB68_13020"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY0050208.1"
                     /translation="MNFKDLGLHPAIVAGCESLGYTEPTPIQQKAIPQVLNGTDIIAC
                     AETGTGKTAAFLLPIIQQLVEKKSKGTTVLVLAPTRELANQTDTACRELAPKKIRCAS
                     IIGGAGYGRQTDALRRGAEIVVATPGRLIDFMERGTIDFSKIETLVLDEADRMLDMGF
                     LPAIKRILKALPIKRQTLFFSATMSPEIEKIARSMMNDPKIIEVSQRGRPTELIEQTA
                     YPVAAQSKMTLLLDLLEREKFERVLVFTRTKRGADRLSHVLEARSHKATRIHGDRSQS
                     QREAALHSFKNGKTRVLVATDVAARGIDIDSVSHVINYDVPEVPEDYVHRIGRTGRAG
                     NKGRAITLVTSIEEPTMRAIERLTGQTVERVLLPDFGGVLPATIRPVQKTASAPTKSF
                     RSFRPRRAR"
     gene            complement(4633..4983)
                     /locus_tag="VGB68_13025"
     CDS             complement(4633..4983)
                     /locus_tag="VGB68_13025"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEY0050209.1"
                     /translation="MKKEKDNKAAEQQTEENEKTGKLSGLGAGVITGAAIGSTIVPVV
                     GTFAGALVGGVVGSEVGKKYGGKLLDKFGSKKSGAADKSDVTAELERLAALHKQGVLD
                     DEEFKAAKASLLNL"
     gene            complement(5090..6655)
                     /locus_tag="VGB68_13030"
     CDS             complement(5090..6655)
                     /locus_tag="VGB68_13030"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEY0050210.1"
                     /translation="MNFFEFLRQNWAELLALTREHIFLVFISTGAAVLLGVPLGVLLT
                     RIKSLQTTVLGIASIMQTIPSLALFGLLIPIPFIGGIGAKTAIIALALYSFLPVIRNT
                     VTGISGVDAKVREAATAMGMTDWQILKTVELPLAAPVILTGIRVAVVIAVGVATVAAA
                     VGAGGLGTYIFRGLRQNDNNLLLAGAISSAILALLADFALGQFEKSFEFKIRVSPSGR
                     KFAAIAATAFVALLLGLSFIPNFSYESANRISDSETASRKIRVGSKDFTESVILAEIL
                     AQILEKKGIAVERQFELGGNLAHDALLANQIDVYPEYTGTAFTAILKHQPVTNPAEVY
                     ERTKREYAGKFNLSLSPPLGFSNDFAILVRGDVARKLNLKTISDAVAHAGSWRAGFGQ
                     DFMSRADGYAGFAKAYNFNFAKQPREMDLSLTYRALASGEVDLIAGNSTDGLIASLDL
                     FQLEDDRRYFPPYEGVFIARADKSEILRETFEKLNKAISTEEMRKLNYEVDGKSLTPR
                     EAANKWLKDKGIL"
     gene            6748..>7308
                     /locus_tag="VGB68_13035"
     CDS             6748..>7308
                     /locus_tag="VGB68_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY0050211.1"
                     /translation="MTKQNFDVNNLRVASPCRVGWETMSGDERVRHCNLCRLNVYNLS
                     EMTADEVRGLITKSEGRICGRLHKRADGTVITKNCPVGFRAYQKRAARMAGAALAAII
                     GLFSVSYGQQEDEKSADASKAKIVRTINRKNILSGSIKDVSGAIVPGAEVVIFKSDDG
                     GEKLKVKSDDEGFYQTPLPAAGIYTLE"
BASE COUNT         1734 a         1948 c         1847 g         1779 t
ORIGIN      
        1 tttaatgtcg tagacgcgat tcgtatcgcc ctcggttttg acggcgacgg tcggaatcat
       61 cacggattgt ttcggcgcgc cctgcgcgat tctaacggtc gcaaattgcc cgggctttaa
      121 taatccttcg ccgttttcga cctcggcttc gaccgtcaaa gtgcgcgaag tagcgttcac
      181 gctcggggaa attctgacga ccgttccggc aaaattgcgg tcgggataag cgctggtttg
      241 cagggaaatt ccctgaccgg ctttgacctg cccgacgttt tgttcgggaa catcaatccg
      301 catccgcaga accgaagtgc gaacaatcgt cgcgaccttt gagctcggcg tgttcggcga
      361 gataaattct ccgacatcgg cgactcgctc ggaaacatag ccgctaatcg gcgagtaaac
      421 aaccgcgtcg ccaatcgctt tttgcgccga cgagatttgc gtccgcgccg cttcttccgc
      481 cgagcgcgcc gtgccgaccg ccgcctgcgc gctgctaacc tgcgtcatcg ccgtgcgaat
      541 cgcgctgacg gcgacggctg cggtcgagcg cgattcgtcg agttgcgccc gcagctggtc
      601 gcgctgcgct ttgcgctggt cgtaaatcgt gcgtgcgatg tcgccggatt ccagaagtct
      661 ttccgcgcgt cccagttctt tttcggctaa ttccagctgg gctttcgtcg cgcgcacctg
      721 cgagaaggtt tcgatgtcga aattgctgcc ttcggtgatg ccgagccgcg actgggtctg
      781 ccgcacgctg gcgcgcgcct gctcgacctg cgcctgcgcc tgccggacgt tcgactgcgc
      841 ctgcgcgacc tgcgcctgcg cctgttccag ccgaatccgc gcgtcgcggt cgtccagccg
      901 caccagcacc gctcctttct gcacgtacga gccgatatcg aaattgacct cgactatttt
      961 tccgccgacc gtcggcgcta cgtccgtctg cgcgtcgctc gccaggctgc ccgtggcttc
     1021 gaaataggtc ggaacattct ggacgatcgc cgtcgcggtc gtcgcctcga cgaccgtcgg
     1081 cagagtgttc gtattggagt tcgcgttatt gttttttgcg ctcgagccgc cgcagctaat
     1141 gataaaaatt gataaaaaac aagctgtcgc ggcaaaaatt ataaatttga ttgatttcat
     1201 aaaacgttcc gaagttttta tgttgttctg ccctttagtt tttaattccc tgaagtaata
     1261 tctccgtgaa atttttcgcc gcttcctcgt tggtaatatc cagcaggcga cgttttctgt
     1321 cccagagcga gttgttcagc gcgtggtgaa tcatcattcc gacaatagcg cgcacgatga
     1381 cgcgcgggtt tatattttgc cgtatcacgc cgtccttttg cctctgcgcg atatacgcgc
     1441 tcataaattc gtaaatcggc acgatgtttt cctcgataaa aatctccgcc agctcgtgac
     1501 cttcgagcgc cgaatgaaac agcagccgca taaattgcgg gtcgccttcg tgcttgttca
     1561 gcgcgttgag aatcatctga taaaaaacct gctcatcgtc tttggcggct aacgattcgc
     1621 acatcgcttc catcgggttg acggctctgc cttcgcaggc tttataatca agaattgcgt
     1681 gataaagctc gtctttggta gcaaaatgcc gaaaaaccat cgcttcggaa acgcccgccg
     1741 cgcgcgcgat ttccttggtc gtcgttccgc gaaaaccgtt ttgtgaaaat aatttaatgg
     1801 cgacctgtaa aatctgtgag cgtcgctcat cgcccgacat tctgccgtgc gtcgtttcgg
     1861 ttgtatccaa ttctttaatc aacaatttct cctcgaactt accagggaag caagtaagta
     1921 agcacttact ttacttaaaa tcaaaaaccg ttgtcaagtc gtatgtgaaa aatttaacaa
     1981 gaactttata aaaaatattt cgtatcaact tttagaagac acagggcatc caaacccaaa
     2041 ccaaagcgac gagttttagt ttggttattt tataaattct gggaaggcaa ggagatttaa
     2101 aaatggctga aatcgaagaa acaaacaacg agcagggctt agtcaagccc aagatttaca
     2161 acggcgagga caaagcggaa aacgattacg tcggcgaatt taaagtacag gcgcaggaat
     2221 acgggcagcg cctgaacgac gcggcgctca aagcgaaaga ttatgcgtcg gaaaagtttg
     2281 atctcgccag cgaaaaattc agggaattgc aggcaaaaga cccgaaggaa ttgattgaag
     2341 acgccaaaga atacgcgcgc cagaaaccgg cgcagacgat tctgatttcg gcggcggtcg
     2401 gcttggttct cggcttgctt ttaaaacgca gataattatt aacgacggga aagagaaaaa
     2461 ggcgattcaa tatttttgaa tcgccttttt ttatataaaa aggttttgaa aagctcggag
     2521 aaccgattat taaaccgtta aaaattgaga cacttcaacc gcaacttcag aatttccgaa
     2581 ctttcaataa tttcaatttg agcaggtaaa aactaaacct gcgcgtgaat caggcggcgc
     2641 actcggtcgc cgaaaagcac gtcgattttc tgcggctcga taacttccat aacctcgccc
     2701 tgtccgaaaa catcgtgaac catcgcctgt cccttgcggt atttgcgcgt ccggtcataa
     2761 gggctgccgg atttggttct gccgaccgtg acggacgttt tcacgtcgcg gctgtaagtg
     2821 ctttccattg cgcaaacgtc acaggtgagc ttggatatct gtccctgttt ggtaacggtc
     2881 aaaaccgaat gatttgtttc ggtatcacaa gccgaacaca tacgctcgac cgaatcgcta
     2941 actgcaaact ttttgcctgc cattttttta tttacctctc tttatctggc gcgtcgcgga
     3001 cgaaaagaac gaaaactttt cgttggagcg gacgcagttt tttgaaccgg acgaattgtt
     3061 gccggcaata caccgccgaa atccggcagt aaaactctct cgaccgtttg tccggtcagg
     3121 cgttcgatag cgcgcatggt cggctcttcg atagaagtga cgagcgtaat ggcgcgacct
     3181 ttatttcccg cgcgtccggt gcgtccgatg cggtggacgt aatcttccgg cacttccggc
     3241 acatcatagt ttatcacatg cgagaccgaa tcgatatcga tgccgcgcgc cgccacgtcg
     3301 gtcgccacta aaacgcgcgt tttgccgttt ttaaagctgt gcagagcggc ttcgcgctgc
     3361 gattgcgagc ggtcgccgtg aatccgcgtc gctttatgcg agcgggcttc aagaacgtgc
     3421 gaaagacggt ctgcgccgcg tttcgtgcgg gtgaaaacca gcacgcgctc gaatttttcg
     3481 cgttccagca aatcgagcag gagcgtcatt ttcgactgcg ccgcgaccgg ataagcggtc
     3541 tgttcgatta attccgtcgg tctgccgcgc tggctgacct cgataatttt cgggtcgttc
     3601 atcatcgagc gcgcgatttt ttcgatttcc ggcgacatcg tcgccgagaa aaagagcgtt
     3661 tggcgcttaa tcggcagggc tttaagaatc cgctttatcg cgggcagaaa acccatatcg
     3721 agcatccggt cggcttcgtc cagaacgagc gtttcgattt ttgaaaaatc aatcgtgccg
     3781 cgctccataa aatcgattaa gcgtcccggc gtggcgacga cgatttccgc gccgcgtctt
     3841 aaagcgtccg tctgacgacc gtagcccgcg ccgccgatga ttgacgcgca gcggattttt
     3901 ttcggcgcca gctcgcggca ggcggtatcg gtctgattcg ccagctcgcg cgtcggcgcc
     3961 agcaccagaa cggtcgtgcc tttcgatttt ttctcgacca gttgttgaat aatcggcagc
     4021 aaaaacgccg ccgttttgcc cgttccggtt tcggcgcagg cgataatgtc cgtgccgttc
     4081 agaacttgcg gaatggcttt ttgttggata ggcgtcggct ctgtatagcc gagcgactcg
     4141 catccggcga caatcgcggg atgcaagcct aaatctttga aattcatgct aaatatttaa
     4201 aattgtaatt ctcgaccgaa atcgaaagcg tctttaatta aacacaactg cccgcgataa
     4261 atttttgagc ggacaaaata cgactaacgc ttcccgaatt tcaggagtta aaaagaagtg
     4321 tctaacgcgg agtgttcctt agtgaaacgg tttgcagcga agggcttgcg ttcgaatgtc
     4381 cggacgaatc cgaacagctt ctatcagtta taaacgaacc gactctcgaa ctatccaaaa
     4441 cgccgccgta agaaatctat caaaggggac gaactaccgt cgaagaaaga gagctggaaa
     4501 aattgtcaat atctatgatg ccttaataat gcgtcgtgtt gcaagctaaa aaactattaa
     4561 gttcaatttt tacaaaaaat aaaagacttt cggaattgtc cgaaagcctt gcccggattg
     4621 atttattaaa ttttataaat tcagcagcga ggctttcgcg gctttgaatt cttcatcgtc
     4681 cagaacgccc tgtttatgca atgccgcgag ccgttccagt tccgccgtga cgtcggattt
     4741 gtcggctgcc ccggattttt tcgagccgaa tttatccagc agcttgccgc cgtatttttt
     4801 gccgacttcg ctgccgacca cgccgccgac cagcgcgccc gcaaaagtgc cgacgaccgg
     4861 gactatcgtc gagccgattg ccgcgcccgt aattacgccc gcgccgagac cggaaagctt
     4921 tccggtcttt tcgttttctt ccgtttgctg ttcggcggct ttgttgtctt tttctttttt
     4981 cattgcgatt tccttttcgt ttttcgtttt aatgaagaat tttgcgggct aatctcgatt
     5041 agcgctcaaa tttaaaaaaa cggctaattg aacgcccgtt tcaaatttat cacaaaatgc
     5101 ctttatcttt cagccattta tttgccgcct cgcgcggcgt caggcttttt ccgtcaactt
     5161 cgtaatttaa tttgcgcatt tcttcggttg aaatggcttt gtttaatttc tcgaaagtct
     5221 cgcgcagaat ttccgattta tctgcgcgcg cgataaaaac gccttcgtaa ggcggaaaat
     5281 accgccggtc gtcttcgagc tgaaacaggt cgagcgaggc aatcaagccg tcggtcgaat
     5341 ttcccgcaat caaatcgact tcgcccgatg ccagcgcccg atacgtcaaa gacaaatcca
     5401 tctcgcgcgg ctgtttggcg aaattaaaat tatacgcttt ggcgaatccg gcgtagccgt
     5461 ccgcgcgcga cataaaatcc tgaccgaatc cggctcgcca gctgcccgcg tgcgcgacgg
     5521 cgtcggaaat cgtttttaaa tttaatttgc gggcgacgtc gccgcggact aaaatcgcaa
     5581 aatcgtttga aaagccgagc ggcgggctca ggctcaaatt aaatttcccg gcatattcac
     5641 gtttcgtccg ctcgtaaact tccgccggat tggtaaccgg ctgatgtttc aaaatcgccg
     5701 taaaagccgt cccggtatat tcgggataaa cgtcgatttg attcgccaga agcgcgtcgt
     5761 gcgccagatt gccgcccagc tcgaactgtc tttcgaccgc gattcctttt ttctccagaa
     5821 tctgcgccag aatctcagcc agaatcacgg attcggtaaa atcctttgag ccgacgcgga
     5881 tttttcggct cgcggtttcg gaatccgaaa ttcgatttgc gctctcgtaa ctgaaattcg
     5941 gaataaagct caagccgagc aaaagggcga caaatgcggt cgccgcgatt gccgcaaatt
     6001 ttcgtccgga cggagaaaca cgaattttga attcgaaaga tttctcgaat tgtccgagcg
     6061 cgaaatccgc cagtaaagcc agaatcgccg aggaaatcgc gcccgccaaa agcagattat
     6121 tatcgttctg acgaagcccg cgaaaaatgt aagttcccag cccgcccgcg ccgaccgccg
     6181 cggcaaccgt cgccacgccg accgcgatga cgaccgccac gcggattccg gttaagataa
     6241 cgggcgcggc aagcggcagc tcgaccgttt tcaaaatctg ccaatcggtc attcccatcg
     6301 ccgtcgccgc ttcgcgcact ttcgcgtcga cgcccgaaat tcccgtgacc gtgttgcgaa
     6361 tgacgggcaa aaacgaataa agcgccagcg cgataatcgc cgttttcgcg ccgatgccgc
     6421 cgatgaacgg aatcggaatc aacaagccga acagcgcgag actcggaatc gtctgcataa
     6481 tgctggcaat gcccaaaacc gtcgtttgca gcgattttat gcgcgtcagc aaaacgccga
     6541 gcggcacgcc gagcaaaacc gccgcgccgg tcgagataaa aaccagaaaa atatgctcgc
     6601 gcgtcaaagc gagcagttcc gcccagtttt gtctgagaaa ctcaaaaaaa ttcatttttc
     6661 gtaatctgga ttgtagcaga aaaaaccggc gtcaaagcaa ctaaaaacgg atttcgccgc
     6721 atcgcttaag cagaaggaag aatcccgatg accaaacaaa attttgatgt taataatctc
     6781 cgcgtcgcct cgccgtgtcg cgtcggttgg gaaacaatgt ccggcgacga gcgcgttcgc
     6841 cattgtaatc tctgccgtct gaacgtctat aacctttcgg aaatgactgc cgacgaggtt
     6901 cgcggattaa tcacgaaatc cgaaggcaga atctgcgggc gtcttcacaa acgcgccgac
     6961 ggtacggtta taaccaaaaa ttgcccggtc ggatttcgcg cttatcaaaa gcgggcggcg
     7021 cgcatggcgg gcgcggcgct ggcggcaatt atcggtttgt tttccgtttc ctacgggcag
     7081 caggaagacg aaaaatcggc ggacgcctcg aaagcgaaaa tcgtcagaac aatcaatcgg
     7141 aaaaacattt taagcggaag cattaaagac gtcagcggcg cgattgtacc aggcgcggaa
     7201 gtggtgattt tcaaaagcga cgacggcgga gaaaagctga aagtcaaatc cgacgacgaa
     7261 ggcttttacc aaacgccttt accggcggcg ggaatttata ctttagaa
//
LOCUS       DASWNE010000667         1539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_127250, whole genome shotgun sequence.
ACCESSION   DASWNE010000667 DASWNE010000000
VERSION     DASWNE010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127250"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..1226)
                     /locus_tag="VGB68_13040"
     CDS             complement(36..1226)
                     /locus_tag="VGB68_13040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEY0050212.1"
                     /translation="MQQKSQKENQSRPIEVTIQKIVSGGYGLAFADGLTVFVALAAAG
                     DRLRVRVREKKGKVAFAEIVEILEPAPDRQTPPCPYFGRCGGCDFQQMNYAAQLAAKA
                     GIVRDCLQRIGKINYENEISIVASPDDLNYRLRAQWHVDSRLEKLGYFRRHSHDVIDV
                     ETCPILAPALKEKLSDIRRDINWKEFWAEKPEIEAATNGAEVSVYSSELIEPTDEIVF
                     EARGEKYYFDARSFFQGNRFLIDDLIRLATAGGEGETALDLFCGVGLFTLPLARKFKK
                     VFGVEANEKAIEFARQNAERAPLSNIEFSAENVSDFLAQSELPKIDFVLLDPPRAGAE
                     KETIYALLKLKPQQISYVSCDPATLARDLSLLTAGYKIESITAVDLFPQTHHVETVVR
                     LNCQ"
     gene            complement(1229..>1539)
                     /locus_tag="VGB68_13045"
     CDS             complement(1229..>1539)
                     /locus_tag="VGB68_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050213.1"
                     /translation="SAEKAPPAVQIRNGEDYMRLTLQSFKRVQLPPDFKYSETINSEK
                     LGNETFYSLDIQRTGYKQRFYATVRKGYSLFFTLTYITAADLETMKTVIRDSDFAWKG
                     "
BASE COUNT          345 a          411 c          400 g          383 t
ORIGIN      
        1 cttttacaat tctcatcctc ttttacaaat tacaattatt gacaattcag acgcactacc
       61 gtctcgacgt ggtgcgtctg cggaaataaa tccaccgcgg tgattgattc gattttgtaa
      121 ccggcggtca aaaggcttaa atctctggct aaggtcgccg ggtcgcagga aacataggag
      181 atttgctgcg gcttgagctt gagcagagcg taaatcgttt ccttttccgc gcccgctcgc
      241 ggcgggtcga gcagaacgaa atcgattttc ggcaattcgc tttgcgctaa aaaatcggag
      301 acgttttccg ccgaaaattc gatattggag agcggcgcgc gctcggcgtt ttgccgcgca
      361 aactcaatcg ccttttcgtt tgcctcgacg ccgaaaactt ttttaaattt gcgcgccaga
      421 ggcaatgtaa ataaaccgac gccgcaaaat aaatcgagcg ccgtttcgcc ttcgccgccg
      481 gcagtcgcca gacgtatcaa atcgtcgatc aaaaagcgat tgccctgaaa aaagctgcgg
      541 gcgtcgaaat aatatttttc gccgcgcgcc tcgaacacaa tctcgtcggt cggttcgatc
      601 agctcgctcg aatagacgga aacttccgcg ccgtttgtcg ccgcttcaat ctccggcttt
      661 tccgcccaga attctttcca gttgatgtcg cggcggatgt ccgagagctt ttcctttaac
      721 gcgggcgcga gaatcgggca ggtttcgacg tcgataacgt cgtgcgagtg acgccggaaa
      781 taaccgagct tttccaggcg cgaatcgacg tgccactgcg cgcgcaggcg ataattcaaa
      841 tcgtccgggc tggcgacaat cgaaatttca ttttcgtaat tgattttgcc tattctttgc
      901 aggcaatcgc ggacgattcc ggctttggcg gcaagctgcg ccgcgtaatt catctgctgg
      961 aaatcgcagc cgccgcagcg cccgaaataa gggcacggcg gcgtttgccg gtcgggcgcg
     1021 ggctccagaa tctcgacaat ctcggcaaag gcgactttgc cttttttttc gcggacgcga
     1081 acgcgcagcc ggtcgcccgc cgccgccagc gcgacgaaaa ccgtcaagcc gtcggcaaaa
     1141 gccagaccgt agccgccgga gacgattttc tgaatggtga cctcgattgg acggctttga
     1201 ttttcttttt gagatttttg ctgcaaattt atcctttcca cgcgaaatcg gaatcgcgga
     1261 taactgtttt catcgtttcc aaatccgcgg cggtaatata agtcaaagta aaaaacagcg
     1321 agtagccctt tcgcacagtg gcgtaaaatc tttgtttata gccggtgcgc tgaatatcga
     1381 gcgaataaaa agtttcgttt cccaattttt ccgagttgat cgtttccgag tatttgaaat
     1441 cgggcggcag ctgcacgcgc ttaaacgact ggagggtcag ccgcatataa tcctcgccgt
     1501 ttctgatttg cacggcgggc ggcgcttttt ccgccgaaa
//
LOCUS       DASWNE010000668         2025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_271294, whole genome shotgun sequence.
ACCESSION   DASWNE010000668 DASWNE010000000
VERSION     DASWNE010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271294"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1820)
                     /locus_tag="VGB68_13050"
     CDS             complement(<1..1820)
                     /locus_tag="VGB68_13050"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY0050214.1"
                     /translation="MKDGCTGQKTGKQRSIPLPVLKNSPAQKDEIRSSKTSRWRAAAL
                     IALTLLMIAHIIQWRLMGQTVTPIEPSEAMHTLQTGAVNAGFIFFSLAILATLIFGRF
                     VCGWGCHVLALQDFCAWLLKKFGITPKPFRSRLLMFVPLIVALYMFVYPTVFRFFVKP
                     KNEPLIPQFTNHLVTSEFWATFPPVWVAIPFLFICGFMTVYFLGQKGFCTYACPYGGF
                     FRLADKVSPGKIRVTDACNGCGHCTAVCTSNVLVHAEVKQYGMVVDQDCMKCMDCVSV
                     CPTEALYFGFGKPAVAVPKTIKKNYSLTWAEEAAAALVFLASFLAVWDVYQLVPMLMA
                     LGIATVTTFLALRTWRLFRAEDLSFHRYNLKMSGKMRRAGWAFMSFALLWVGLNAHSG
                     WVHYHESAGGRAYQNIKIPDELALARTNPDRWLSPSDRANIVEGKKQFHAAMNAGLLV
                     NSTALAKLAWIEYLSGNTEQAVNLLGDAAEHRQSQGKALSYYYRGAILNRLGRYEQAL
                     ASLDQALAERADLIVAHEERGESLWQLGRREEAVSAWSNAVQSNAGLALANNLLAGAA
                     ASMGKPEAAVAFENQADRSTPADPLFHWILGLRLQNVGMN"
BASE COUNT          510 a          544 c          532 g          439 t
ORIGIN      
        1 tggttcatcc cgacgttttg cagccgaagt ccgagtatcc aatgaaaaag cgggtctgcg
       61 ggagtggagc ggtcggcttg gttttcaaaa gcgactgccg cttccggttt gcccatagac
      121 gcggcagcgc cggcgagcag attatttgcc agcgcgagac cggcattgct ctgcacggca
      181 ttgctccagg ctgaaacggc ttcctcgcgg cgtccgagct gccagagcga ttcgcctctt
      241 tcttcatgag cgacaattaa atcggctcgc tccgccaaag cctggtcgag actcgccagc
      301 gcctgctcgt aacgccccaa acggttgaga attgcgccgc ggtaatagta gcttaaggct
      361 ttgccctgcg attgccggtg ttcggcggcg tcgccgagca aatttacggc ttgctcggtg
      421 ttgccggaca ggtattctat ccacgcaagt ttcgccagcg ccgtgctgtt tacgagcaag
      481 ccggcattca tagcagcgtg aaattgtttt tttccttcga cgatgttcgc gcgatcactc
      541 ggactgagcc atcggtccgg attggttcga gccagagcga gctcgtcggg aatcttgatg
      601 ttttgatatg cgcgcccgcc cgcggattcg tggtaatgca cccagccgct atgcgcgttt
      661 aagccgaccc acagcagcgc gaaactcata aatgcccatc ccgctcttcg cattttgccg
      721 gacatcttca aattataacg gtggaaagac aagtcttcgg ctcggaataa tcgccacgtt
      781 ctcagagcga gaaacgtggt gaccgtggca attccgagcg ccatcagcat cggaacgagt
      841 tgataaacgt cccagacggc taaaaagctc gccagaaaca caagcgccgc cgccgcttct
      901 tcagcccagg tcagagaata gtttttctta atcgttttcg gaacggcgac agccggtttc
      961 ccgaacccga aatataaagc ttccgtagga cagacgctga cgcagtccat acacttcata
     1021 cagtcctgat cgacgaccat cccgtattgt ttcacctccg cgtgaaccag aacgttcgaa
     1081 gtgcagacgg cggtgcaatg tccgcagccg ttgcaggcgt cggtaacgcg gatttttccg
     1141 ggcgagacct tatcggcgag tctgaagaag ccgccgtaag gacaggcgta ggtacagaaa
     1201 cccttctgcc ccaaaaaata gacggtcata aatccgcaga tgaagagaaa aggaatggcg
     1261 acccagacgg gcgggaaagt cgcccaaaat tcgctggtca ccagatgatt ggtaaactgc
     1321 ggaataagcg gctcgttctt cggcttgacg aaaaagcgaa acacggtcgg ataaacgaac
     1381 atatacagcg ccacgatgag cggcacgaac attagcagcc gcgaccgaaa cggcttcggc
     1441 gtaatcccga atttcttcag cagccaggcg caaaaatcct gtagagcgag aacgtgacag
     1501 ccccagccgc agacaaaacg cccgaaaatc agcgttgcca gaatcgccag gctaaagaat
     1561 ataaatcccg cgttgacggc gccggtttga agcgtatgca tcgcttcgga aggttcaatc
     1621 ggcgtaaccg tctgacccat cagtcgccat tgaatgatgt gcgcgatcat cagcaaggtc
     1681 agagcgataa gcgccgccgc ccgccagcgg ctggtcttcg agctgcgaat ctcgtctttt
     1741 tgagccgggg agttttttaa aacaggcagt ggaatcgacc gctgtttccc tgttttttga
     1801 cctgtacatc catctttcat aaatcgattt gagttctgcg cctgagaggt tgacagaaaa
     1861 acatattata cccgacttga ttaaagtacc taaaagggtg tataagaaaa gtctaaacct
     1921 gcgtaactga aaagttaaaa tccacaataa ctgaaaagtt ataatctact ggaattgata
     1981 caaactagag gtaatcattt tgtacagccc aactggctgg aaatc
//
LOCUS       DASWNE010000669         7954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_481473, whole genome shotgun sequence.
ACCESSION   DASWNE010000669 DASWNE010000000
VERSION     DASWNE010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7954
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481473"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VGB68_13055"
     CDS             <1..579
                     /locus_tag="VGB68_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050215.1"
                     /translation="ILSKVKAYLGAGGIEQIFNLSGAQGRFEKFLEATAKRHGELAQV
                     FPKIFRHQDKLSQILRQRALVTNAEHRFFLALLLNVEGKERIFSLVTERFPESDALEK
                     ILDWTYDLAQTKVFGSNMPNALGVEDFDDMDLFVLESLLKDRSEAEMLEALQTDYGTE
                     ATGETSRNLSAKAEKIRQAVIFEPLLGLGNKD"
     gene            complement(797..3103)
                     /locus_tag="VGB68_13060"
     CDS             complement(797..3103)
                     /locus_tag="VGB68_13060"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEY0050216.1"
                     /translation="MKQVGTTKRNRLKNAWVLMFALAWVILPFALNKTVVQADDLSDD
                     IRIRASMTGPAINGITPSGFAEHRTEDDGRRRLDVDVFSVSLPAGTVLNVVLNNAVIG
                     QVTLDGFGNAFFRRDTNNGQTVPPANNGNPIAVMNGNTTIISGVFGVVTPTPSPSGSP
                     TGSPTASPSVSPTGSPTGSPTASPTVSPTVSPSVSPTASPTGSPNPSPTVSPSPNVSP
                     SPSPGGNGDLFAGLTGPTLNGVLPNGFAQFEFHSSRIELEVRVRQVNLPGGTMLNVVV
                     NNATVGQMFISGGEGRLRLRSDEGQNVPVITAGSTIIIFNGSSAILSGIFSGASPSPS
                     PSPSVSPTGSPTGSPSPSPSVSPTVSPSPNVSPSPSPSVSPSPSPSPAQGRYFEGNLS
                     STVNNGSRGELKIFLNATETQAQVTGEFNLSSQQTTAKILSDIGGVVVHDFGTVGGTQ
                     GQVNVTFNVTPAQVQQIRAGLWYAVFGSVANPNEVRGVIRNSSRSSDFDGDGVVDMAV
                     FRPQDGAWYIQNSTGFSATTFGGVNDKLVSGDFDGDGRSDAAVYRSNANGAGIWDIKR
                     SSDGGQTTEQFGLSTDIPVRGDYDGDGLQDLAVFRPSNGYWYIKRSTDAGYTIVQFGQ
                     NGDKPVPADLDGDGKTDIAVFRPNVGDWYWLGSRDGQFRGLHFGVSEDLPIAGDFDGD
                     GRDDLTVYRPTTGSWYRINSSNDQFVAIQFGISTDVPVAGDYDKDGKMDIAVFRKAEG
                     AWYILRSSDGAFEARFFGLNGDIPIPAQ"
     gene            complement(3308..4732)
                     /locus_tag="VGB68_13065"
     CDS             complement(3308..4732)
                     /locus_tag="VGB68_13065"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY0050217.1"
                     /translation="MNRFIENNWLKACFVLLSIFIISGSGFGQSDNCNCEPNEILIDL
                     VNQNDLSAVAAQYKLIQTPISQVGTPPTYLMRINRDPVTGTTQTPLQIAAAMQNDARI
                     RQREVNRKINLAERPGLSWTLGRSWAIGRSWAIGGSSKGFNSQYFPDVIKLNQAHTIS
                     TGNNMVVAVLDTGIDLNHPAFAGRLVPGYDFVDNDNDPSEVGVLHQNPVYGHGTHVAG
                     IIALTAPNARIMPLRVLNTEGEGNLWRIMTAMLWAARNNNSTIDTTVINMSFGYPADL
                     TAQSNSFLHDLFTSCGELPPVPGQQQFCGAEDDALLIVAGAGNGGQIGNGSARIYPAA
                     ERGGVNDIDDNLLSVGASTRYDRLASFSTMANVVNRDVDRWVRTVAPGEDIISALPGG
                     RYGMWSGTSMATPIVAGVAALVKSANPNMQLTDLVERIEETGYEWDCIVQSRNIRMET
                     ARVDAYCALTNNEACYAPRTVCPE"
     gene            complement(5082..7892)
                     /locus_tag="VGB68_13070"
     CDS             complement(5082..7892)
                     /locus_tag="VGB68_13070"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEY0050218.1"
                     /translation="MLRANLAEKLISAGSEPERKRLLIESNKYADVKLAFALKEICYA
                     AWTTEPVKAQKAAQALKTLVKINPNEEISALSFWVSGIAELTGGRLESAVRNLDRAAE
                     IFFQIGREHDAAQTQVAKLIALAMLGRYDEAIETSKKTLKIFEKYGDELAAGKTEMNL
                     SNIVSRREMHAKAEKYCLSARKRFLKTGEKTWLTMAENGLANTYTELNDFQKAEKFYA
                     SALQNARESKMLVTEAEIEASRGNLALFRGRFDEALKSLELSRQIYETLEMPHQTAIA
                     ELEIADIYLELNLAKEAFSIYEKITEELRKLKLQGEEARARANFGRAAAVLNETKRAR
                     GQLKKSAQLYRREKNKVGAAVVKLTEANLEIEQGNFKKALRLADESANLLAGSENLRH
                     RLMNGWIQAEALRNLKDFKKAETLLNEVFTAAVKREQPQIVHLALNSLGKLFWQKGDT
                     RQAEKFFKKAIKLIETLRSPLPAEEFRMAFLANKLAPFENLARIYLAENKIKAAFLMI
                     EKARSRSLAESIADGNLQPAKNTASTRLTRRLEALREELNWFYSRLSRAENAEIANLQ
                     REAQKREKQIADVLRQIESTGENSAAKSESFDFAKLQKQIGGDKALIEFVSFDNCLSA
                     FVVTNKKIHFVKNLAGETKIFAFLEGLRFQFGALRYGAKSLGKFIDELKKRADFYLAK
                     LYACLVKPLEELLGNRDLAIVPVGVLHYVPFHALLRGRDEKYLIETRKITYEPSATVW
                     QFLEKKPARKIKNALLIGFADEKIPLVNREIETLKKIFPKAKTFSGKAATFEAYTKNA
                     PQFEVLHLACHGQFRPENPLFSSLHLADGWITVRDICAQRLNAGLVTLSACETGLNEI
                     FAGDEILGLARGFLTAGALSLVLSLWTVNDEATTALMREFYTQLRAGKETSKALQLAQ
                     CSFIRENAHPYFWSPFLLIGKF"
BASE COUNT         1779 a         2122 c         2050 g         2003 t
ORIGIN      
        1 attctttcaa aagtcaaagc ttacctcggc gcgggcggaa tcgaacaaat tttcaatctt
       61 tccggcgcgc agggtcgttt tgaaaaattt ctggaagcga ccgcgaaacg tcacggcgag
      121 ctggcgcaag tgtttccgaa aattttccgg catcaggaca agctcagtca aattttgcgc
      181 cagcgcgcgc tggtgacaaa cgccgagcat cgtttttttc tggcgctgct tttgaacgtc
      241 gaaggcaagg agcgaatttt ctcgctcgtc acagagcgtt ttcccgagag cgacgcgctg
      301 gaaaaaattc tggattggac ttatgatttg gcgcagacca aagttttcgg ctcgaatatg
      361 ccgaacgcgc tcggcgtcga agatttcgac gatatggatt tgttcgtgct ggaaagtctt
      421 ttgaaagacc gctcggaagc tgagatgctg gaggctttgc aaaccgatta cggaaccgag
      481 gcgaccggcg aaacgtcgcg caatctttcg gcaaaagcgg aaaaaattcg tcaggcggtg
      541 attttcgagc cgcttctggg tttagggaat aaagactgaa tcgagtgcta ttctgtttat
      601 tcctggttca gcctttatcc ctttaaaaaa agccccgacc gcgaggaggg caaagcagtc
      661 agggcttgca gttaactcca aattcccgtt cggagttaaa attattcaaa gcaactgttt
      721 gatgttttac gaccgattac tgttgctttg acgagaaaga ggctgagcgt ataactcagc
      781 ctctttcgcc tttttactac tgagccggaa tcggaatgtc gccgtttaag ccgaagaatc
      841 tggcttcaaa agcgccgtcg ctgctgcgca gtatatacca cgcgccctca gctttgcgga
      901 acaccgcgat gtccatttta ccgtccttgt cgtagtcgcc cgcgaccgga acgtcggtgc
      961 tgattccgaa ctgaattgcg acgaactggt cgttcgagct gttaatgcga taccagctac
     1021 cggttgtcgg gcgatagacc gttaagtcgt ctctgccgtc gccgtcgaag tcacccgcga
     1081 tgggcaaatc ttcgctgacg ccgaagtgca atccgcggaa ctgtccgtcg cggctgccga
     1141 gccagtacca gtcgccgaca ttcgggcgaa agaccgcgat gtccgtttta ccgtcgccgt
     1201 ccaaatctgc cggaacgggc ttgtcgccgt tctgtccgaa ttggacgatg gtgtaaccgg
     1261 cgtcggtcga gcgcttgata taccagtaac cgtttgacgg acggaagacc gctaaatcct
     1321 gcagaccgtc gccgtcgtaa tcgccgcgca ccggaatatc cgtgctgagt ccgaactgtt
     1381 cggtcgtctg accgccgtcc gagctgcgtt tgatgtccca gatgcccgcg ccgttggcgt
     1441 tgctgcggta aacagccgcg tcggagcgtc cgtcgccgtc aaagtcgccg gagacgagct
     1501 tgtcgttgac gccgccgaaa gtcgtggcgc taaagccggt gctgttctgg atataccacg
     1561 cgccgtcctg cgggcggaag acagccatat cgaccacgcc gtcgccgtca aagtcgctgc
     1621 ttctcgaact gttgcggatt acgccgcgca cttcattcgg attagccacg ctgccgaaga
     1681 ccgcatacca caatcccgcg cgaatctgct gaacctgtgc cggagtcacg ttaaaggtca
     1741 cattcacttg tccctgcgtg ccgccgacgg tgccgaagtc gtggacgacg actccgccga
     1801 tgtcagacag aattttggcg gtcgtctgct ggctcgacag attgaattcg cccgtaacct
     1861 gcgcctgtgt ttccgtcgcg ttcaagaaaa tcttgagttc gccgcggctg ccgttgttga
     1921 ccgtgctgct caaattacct tcaaaatatc tgccctgtgc gggagacggt gaaggtgaag
     1981 gcgagaccga cggtgacggc gaaggcgata cgttcggcga cggtgaaaca gtcggcgaaa
     2041 ctgacggtga aggtgaaggc gagccggtcg gcgaaccggt cgggctgacg ctcggactcg
     2101 gcgacgggct cgggctggcg ccggaaaaga ttccgcttaa aatagccgag ctgccgttaa
     2161 aaatgataat cgtcgagccg gcagtgataa ccggaacgtt ttgaccctcg tcgctgcgga
     2221 gtctgagtct gccctcgccg ccgctgatga acatctgtcc gaccgtcgca ttattaacga
     2281 cgacgttcag catcgtgccg cccggcagat tgacctggcg cacgcgaact tcgagttcga
     2341 tgcggctgct gtgaaattca aattgagcga aaccgttcgg cagaactccg ttcagcgtcg
     2401 gaccggttaa gccggcaaac aaatctccat taccgcccgg cgaaggagaa ggcgatacgt
     2461 tcggcgacgg cgaaactgtc ggcgacggat tcggtgagcc ggtcggcgaa gcggtcgggc
     2521 tgacgctcgg cgagacagtc ggcgagacag tcggagaagc ggtcggcgaa ccggtcggac
     2581 ttcccgtcgg gctaacgctc ggcgaagccg tcggcgagcc ggtcggcgaa ccagaaggcg
     2641 aaggcgtcgg cgtaacaacg ccgaagacgc cggagataat agtcgtgttt ccgttcataa
     2701 ccgcaatcgg atttccgttg ttggcgggcg gaacggtttg accgttattc gtatcgcggc
     2761 ggaaaaaagc gttgccgaaa ccgtcgagcg tcacttgtcc gatgaccgcg ttgttcagca
     2821 cgacgtttaa aaccgttcct gccggtaaac tgaccgaaaa aacgtcgaca tccagcctgc
     2881 gccgtccgtc gtcttccgtg cggtgttcgg caaagcctga cggggtgatg ccgttaatcg
     2941 ccggtcccgt cattgaagcg cgaattctta tgtcatcgct caaatcatct gcctgaacaa
     3001 ccgttttatt tagagcgaac ggcaggatta cccatgccag cgcaaacatc agcacccatg
     3061 catttttgag ccgattgcgc tttgttgttc ctacttgttt catattttga taccttcctt
     3121 aactacctgt ttaattcgtt tgtctatcgt ttctcgaatg cctttaaagc gatttgtttg
     3181 ctttgaaata tggctttctg ctatacagtg ataaaaatat tgtgaaaaag acaaaattga
     3241 tgattttttt ttcaaataaa aagccaatcg tttccaattg gctttttatt taatttacta
     3301 ttaaattcta ctcgggacag accgttcgcg gagcataaca ggcttcgtta ttcgttaatg
     3361 cgcagtaagc atcgactcgg gctgtctcca ttctgatatt tctcgactgc acgatgcagt
     3421 cccattcata gccggtttcc tcgattcgtt ccaccaaatc cgtcagctgc atattaggat
     3481 tggcggattt caccagagcc gcgactcccg ccacaatcgg cgtcgccatc gaagttccag
     3541 accacatacc ataacgcccg cccggcagcg cgctgattat atcttcgccc ggcgcaaccg
     3601 tgcgcaccca gcggtcgaca tctctattta ccacattagc cattgtcgaa aacgacgcca
     3661 gccggtcata gcgcgtgctt gcgccgacgc tcagcagatt gtcgtcgata tcgttgacgc
     3721 cgccgcgctc ggctgccgga taaattctcg ccgagccgtt accgatttgc ccgccgttgc
     3781 cggctccggc aacaattaaa agcgcgtcat cctctgcgcc gcagaattgc tgctgccccg
     3841 gaacaggcgg aagctcgccg cagctggtaa acaaatcgtg cagaaacgaa ttgctctgcg
     3901 cggtcaaatc cgccggatag ccgaaactca tattgatgac ggtcgtatcg atggtcgagt
     3961 tgttgtttcg cgccgcccag agcatcgcgg tcataatccg ccagagattt ccctcgcctt
     4021 ccgtgtttag aactcgcagc ggcataatcc tggcattcgg cgcggtcaag gcaatgattc
     4081 cggcgacgtg cgtgccgtgc ccgtaaaccg gattctgatg cagaacgccg acctcgctcg
     4141 ggtcattgtc gttatcgacg aaatcgtagc ccggaaccaa tcttccggca aaagccggat
     4201 gatttaaatc aattccggtg tccaaaaccg cgacgaccat attatttccc gtcgaaatcg
     4261 tatgcgcttg attgagtttt atgacgtcgg gaaaatattg actgttaaag cctttcgagc
     4321 tgccgccgat tgcccacgag cgtccgattg cccacgagcg cccgagcgtc cagctcagtc
     4381 cgggtcgctc cgccagattt atttttctgt ttacttcgcg ctggcgaatt cgcgcgtcat
     4441 tttgcatcgc cgcggcgatt tgcagaggag tttgcgtggt tccggtgacc gggtcgcggt
     4501 ttatgcgcat cagataagtc ggcggagtcc cgacctgtga gattggcgtc tggataagct
     4561 tatactgcgc ggcgacggct gataaatcgt tttgattaac caggtcgatc aaaatctcgt
     4621 tcggctcgca gttgcaatta tcgctctgcc cgaaaccgct gccggaaatg ataaaaatcg
     4681 aaagcaagac aaaacacgct ttcaaccagt tattttcgat aaacctgttc attgctgacc
     4741 tcataaacac aaatccccta tacgtattgc cgattacaca gtgcggttag tcccggcaaa
     4801 aagacactct aaatcacttt ttctaaaaaa attagtaagg aataaaagcc ggaggtaacg
     4861 gaaaatcatt accgggctat ccgcagatac aaaaaccgcc gggaaaatta atactccccg
     4921 aaacctattc tttaaaagcg tggcaaagct catacatttt tctccctcgc ccgctgaacg
     4981 gatttatagt cgagggatgc cgatagattt taagtaatcg ggattttagc caataaaccg
     5041 ggaaaaacca atatttagat tggtttaaga ataaggaaaa gctaaaattt tcctattaat
     5101 aaaaaaggcg accaaaaata ggggtgagca ttttccctga taaaactaca ctgtgcgagc
     5161 tgtaaggctt tagatgtttc ctttccggct ctgagttgcg tgtaaaattc ccgcatcaga
     5221 gccgttgtcg cctcgtcgtt gaccgtccag aggctcaaaa cgagagagag agcgccagcc
     5281 gtcagaaatc cgcgcgccag tccgagaatt tcgtcgcccg caaaaatttc gttcaagccg
     5341 gtttcgcacg cgctcagcgt caccagcccg gcgtttaatc tctgcgcgca gatatcgcgc
     5401 acggttatcc agccgtcggc gagatgaagg ctggaaaaca gtggattttc cgggcgaaac
     5461 tgcccgtgac aggcgagatg cagcacttca aactgcggcg cgttttttgt gtaagcctcg
     5521 aaagtcgccg ctttgccgga aaaggttttc gctttcggga aaatcttttt gagcgtttcg
     5581 atttcgcgat tgacgagcgg aattttctcg tcggcaaagc cgatcagcaa ggcgtttttg
     5641 atttttcgcg ccggcttttt ctctaaaaac tgccagacgg tcgcgctcgg ctcataagtg
     5701 atttttcgcg tttcaatcag atatttttcg tcgcgcccgc gcaggagggc gtgaaacggg
     5761 acgtaatgca gcacgccgac cggaacaatc gccaaatcgc gatttcccag cagctcttcg
     5821 agcggtttga ccagacacgc gtaaagcttc gccagataaa aatcggcgcg ttttttcagc
     5881 tcgtcaataa attttccgag acttttcgcg ccgtaacgca gcgcgccgaa ctgaaaccgc
     5941 aagccttcga gaaacgcgaa aatcttcgtt tctccggcta aattcttaac aaaatgaatt
     6001 ttcttattcg tgacgacaaa cgccgaaaga caattatcga aactgacgaa ttcgattaag
     6061 gctttatcgc cgccgatttg tttttgcagc ttggcaaaat cgaaagattc gctctttgcg
     6121 gcggagtttt cgccggtgct ttcgatttgc cgcaaaacgt cggcgatttg tttttcgcgc
     6181 ttttgcgctt cccgctgcag attggcgatt tccgcgtttt cggcgcggct caggcggctg
     6241 taaaaccagt ttaattcctc gcgcagagct tccagtcttc tggtcagcct ggtcgaagca
     6301 gtgttttttg cgggctgcag atttccatcg gcgattgatt ccgccagcga gcgcgagcgc
     6361 gctttttcaa tcatcagaaa cgccgctttg attttatttt ccgccagata aatccgcgcc
     6421 agattttcaa acggcgcgag cttatttgcc agaaacgcca tccgaaattc ctccgccgga
     6481 agcggcgagc gcagcgtctc tatcaattta atcgcttttt tgaaaaactt ttcggcttgc
     6541 cgcgtatcgc ctttttgcca gaataatttt ccgagcgagt tgagcgccag atggacaatc
     6601 tgcggctgtt ctcttttcac agcggcggta aatacttcgt ttaaaagagt ttccgctttt
     6661 ttgaaatcct tcaggtttct taaggcttcg gcttgaatcc agccgttcat taatcgatga
     6721 cgcagattct cgctgccggc gagcaaattt gcggattcgt ccgccagccg gagagctttt
     6781 ttaaaatttc cctgttcgat ttccagattg gcttcggtta atttgacgac cgccgcgccg
     6841 accttgtttt tttcccggcg gtaaagctgc gcggattttt ttaattgccc gcgcgcccgt
     6901 ttcgtttcgt tcaaaacggc tgccgcgcgt ccgaaattgg cgcgcgcccg cgcttcttcg
     6961 ccctgcaatt ttaattttcg cagctcttcg gtgatttttt cgtaaatcga gaaagcttcc
     7021 tttgccagat tcaattccag atagatatcg gcgatttcca gttcggcaat cgcggtttga
     7081 tgcggcattt ccagcgtttc gtaaatttga cgcgatagct ccagcgactt taaggcttcg
     7141 tcgaaccgcc cgcgaaaaag cgcgagattg cccctgctcg cttcgatttc cgcttcggtg
     7201 accagcattt tcgattcgcg cgcgttttgc agagcggaag cgtagaattt ttccgccttt
     7261 tgaaaatcgt tgagttcggt ataggtattg gcgagcccgt tttcagccat cgtcagccac
     7321 gttttttcgc ccgttttcaa aaaacgtttg cgcgccgaaa gacaatattt ttcagctttc
     7381 gcgtgcattt cgcggcgcga gacgatattg ctcagattca tttcagtctt gcccgccgcc
     7441 agctcgtcgc cgtatttttc aaaaattttc agcgtctttt tgctcgtttc gattgcttcg
     7501 tcatagcgcc cgagcatcgc gagcgcgatg agcttggcga cctgcgtctg cgccgcgtcg
     7561 tgttccctgc cgatttgaaa gaaaatttct gctgcgcggt caagatttct gacggctgat
     7621 tcgagcctgc ctccggttaa ttcggcgatg cccgaaaccc agaacgaaag cgccgaaatt
     7681 tcttcattcg gattgatttt gacgagagtt ttcaaggctt gcgccgcctt ttgcgctttg
     7741 accggctcgg tcgtccacgc cgcgtagcag atttccttta aagcaaaagc aagttttacg
     7801 tccgcgtatt tattactttc aatcaaaaga cgcttgcgtt ccggctcgct tccggcggaa
     7861 attaattttt cggctaaatt agcgcggagc atataaaaat atattaacaa taagaaaaat
     7921 tcatcacaaa gcggcgcgga tgaatgaaaa accg
//
LOCUS       DASWNE010000670         2863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_658811, whole genome shotgun sequence.
ACCESSION   DASWNE010000670 DASWNE010000000
VERSION     DASWNE010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658811"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..627
                     /locus_tag="VGB68_13075"
     CDS             85..627
                     /locus_tag="VGB68_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050219.1"
                     /translation="MKTANGNEFLFIALCLFTFAFCFPASALAQPEDIEVAPPPMKFI
                     SKEEKQQLEGEPNVKKHTLLALSLMEARLKTAETLTTENNYREALNTLGGFQILLENT
                     LSFLSKNNIENDKVQSNLKKLELTLREQTSRLEVMRRAMPSRYAFYVHKLIRIVRDAR
                     SKAIEPLFDDTVVPQSQLKP"
     gene            648..1220
                     /locus_tag="VGB68_13080"
     CDS             648..1220
                     /locus_tag="VGB68_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050220.1"
                     /translation="MRSAKFARPKTLFFSLTLLLVAYCSLATVDAQRRDYLTEQEADL
                     VREAQELDRRIDILTKAIDRRFLVLSGRQAELKDVEKWGELKGTKAALLFDISRILQS
                     AIDNIEYVAEKDASNKLFSKSIHTLADASRKYLPQLEIYKSRLNEKMEQAAILNSIDF
                     CNQIIEASARVPKDAPKEEKKKKSSKDESN"
     gene            complement(1422..1544)
                     /locus_tag="VGB68_13085"
     CDS             complement(1422..1544)
                     /locus_tag="VGB68_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050221.1"
                     /translation="MFTNFVRLIKLESFFRLLRAGASLPDFGFEVIFAARGRFG"
     gene            1628..2737
                     /gene="gcvT"
                     /locus_tag="VGB68_13090"
     CDS             1628..2737
                     /gene="gcvT"
                     /locus_tag="VGB68_13090"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEY0050222.1"
                     /translation="MENLKKTPLNEAHRAAGGKMVEFGGWDMPVQYTPGVIEEHLRTR
                     TSAGLFDVSHMGEIWVEGADAIEFVNRLTTNDVTKLVDGQAQYSALTNENGGVVDDLL
                     VYRFDFDKLLLVVNASTTEKDWDWITSHKKDEVITLTNASKDYCQIAVQGPRALNILQ
                     RLTDAELSDIKYYYFTTGKVDGVDSIISRTGYTGEDGFEVYAAPQYARQIWDSLLETG
                     NYGEADGILPCGLAARNTLRLESAMSLYGHELGDEITPLEAGLGWICKLNKGEFTGRA
                     SLARQKQEGIKRKLVGFEMIDRGIGRDGFDVYISGEKVGFVSSGSPAPYLKKNIGLAF
                     VPAEFANVGQEIKIDVRGKHLAAQIVPTPFYKRGK"
BASE COUNT          797 a          700 c          722 g          644 t
ORIGIN      
        1 ggaagaaagg aaaaaacgat gagaggtaaa aatgtcaaaa gtctccggtt cgggattata
       61 aaattccgtt tcttgaacgg cgaattgaaa accgcaaacg gcaacgagtt tctgtttatc
      121 gccctttgcc tttttacttt cgctttttgt tttccggctt ccgctctggc acagcccgaa
      181 gacatagagg tcgcgccgcc gccgatgaaa ttcatctcga aagaggaaaa acaacaactc
      241 gaaggcgagc cgaacgtcaa gaaacacact ctgctggctt taagcctgat ggaagcgcgg
      301 ctgaaaacag ccgagacgct gaccaccgaa aacaattatc gcgaagcttt aaacacgctc
      361 ggcggctttc agattttgct tgaaaacacg ctcagttttt tatcgaaaaa caatatcgaa
      421 aacgataaag tccagagcaa tctgaagaaa ctggagctca ccttgcgcga gcagacttcg
      481 cggctggaag tgatgcgccg cgcgatgcct tccagatacg ctttctacgt gcataagtta
      541 atcagaatcg tgcgcgatgc gcgaagcaaa gccatcgagc cgctttttga cgatacggtc
      601 gtgccgcagt cgcagctaaa accatgaaaa taaatttaaa acgccaaatg cgaagcgcaa
      661 aattcgcgcg cccgaaaacg ctctttttct ctctgacttt attgctcgtc gcttattgct
      721 cgctggcgac ggtcgacgcg cagaggcgcg attatctgac cgagcaggaa gccgacctcg
      781 tccgcgaggc gcaggagctc gaccgccgga ttgacattct caccaaagcg attgaccgcc
      841 gctttctggt tttgagcggt cggcaggcag agctcaaaga cgtcgaaaaa tggggcgagc
      901 tgaaaggcac gaaagccgcg ctgcttttcg acatctcgcg aattttgcag tcggcgattg
      961 acaacatcga atacgtcgcc gaaaaagacg cgagcaataa actgttttcg aaatccatcc
     1021 acactctcgc cgacgcgagc cgcaaatacc tgccgcagct ggaaatttac aaaagccgcc
     1081 tcaacgaaaa aatggaacaa gccgcgattc tgaattcgat agatttctgc aaccagataa
     1141 tcgaggcttc ggcgcgagtg ccgaaagacg cgcccaaaga agaaaagaaa aagaaatcct
     1201 cgaaagacga atcgaattaa acaaagacga atcgaattaa aaataaaaag gcgagcgatg
     1261 acggctcgct tttttatttt aattcgaatt tttcgtttat aacgccgaaa tattcgagaa
     1321 attaagaagt cgaaagattt ttcgccggat taaagaaatt cgacgtatat ccggcttccg
     1381 acgagccgcc cggcaattcc gccgaaaaac tttttcgccg tttagccgaa gcgcccgcgc
     1441 gcggcgaaaa ttacttcaaa gccgaaatcc ggcagggacg cgccggcgcg cagcaatcgg
     1501 aaaaacgatt cgagtttgat tagccgcaca aaattcgtaa acaaattttt ctcatttatc
     1561 gcatcttgac gcttgaagcg tcgggtgtta gtgttttttc tttacggtct tcttttgcgg
     1621 aaaatctatg gaaaacttga agaaaacgcc gctcaacgaa gcgcatcgcg cggcgggcgg
     1681 aaaaatggtc gagttcggcg gctgggatat gcccgtgcaa tacacgccgg gcgtcatcga
     1741 ggaacatctg cgcacgcgca ccagcgccgg acttttcgac gtttcgcata tgggcgagat
     1801 ttgggtcgaa ggcgcggacg ccatcgaatt cgtcaatcgt ctgacgacga acgacgtcac
     1861 gaagctggtt gacggacagg cgcagtattc ggctttaacg aacgaaaacg gcggcgtggt
     1921 tgacgattta ctcgtttatc gttttgattt cgacaagctt ttattagtcg tcaacgcttc
     1981 gaccaccgaa aaggattggg attggattac ctcgcataaa aaagacgaag tcatcacctt
     2041 gaccaacgcc agcaaagatt actgccagat tgccgtgcaa ggtccgcgag ccttgaacat
     2101 tttgcagcga ttgaccgatg ccgagctttc cgacatcaag tattattatt ttacgaccgg
     2161 aaaagtcgac ggcgtcgact cgattatttc gcgcaccggc tacacgggcg aagacggttt
     2221 tgaagtttac gccgcccctc agtatgccag acaaatctgg gacagcctgc tcgaaacggg
     2281 aaattacggc gaggcggacg gcattttgcc gtgcggtctg gcggcgcgca acactctgcg
     2341 gctggaatcg gcgatgtcgc tttacggtca cgagctgggc gacgaaatca cgccgctcga
     2401 agcgggttta ggctggattt gcaaattaaa caaaggcgaa tttaccgggc gcgccagttt
     2461 ggcgcggcaa aagcaggaag gcatcaagcg caagctcgtc ggctttgaaa tgattgaccg
     2521 cggaatcggg cgcgacggtt tcgacgttta catctcgggc gaaaaagtcg gcttcgtttc
     2581 ttccggctcg cccgcgcctt acctgaaaaa gaacatcggc ttggctttcg tcccggctga
     2641 gtttgcaaat gtcggtcaag aaattaaaat cgatgtcaga ggcaaacatc tggcggcgca
     2701 aattgtcccg acgccgtttt ataaaagagg aaaataattt tagatttgcg attgccggtt
     2761 ttcgattgga acataaaaaa tttattttct ttccgatcgc aaatctaaaa tctaaaatct
     2821 aaaatctaaa attaatttat ggcaaacatt ccggaaaatt tac
//
LOCUS       DASWNE010000671         5289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_199727, whole genome shotgun sequence.
ACCESSION   DASWNE010000671 DASWNE010000000
VERSION     DASWNE010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199727"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="VGB68_13095"
     CDS             <1..931
                     /locus_tag="VGB68_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050223.1"
                     /translation="TYGNLELPSGRLAKISRNDVLFGVLGKRRALKGFVGDVPASVKA
                     GDRLHLLNMGGLIGVCKGHHSSLSEAIEVEVLGIACDFEIAPQQARVLNIADRALKPC
                     NFLKECAPVVIVAGTCMNSGKTVAATEIIKQATHAGLKVAGAKMSGVAALRDTLNMQD
                     HGAAATASFLDCGLPSTVDVEDLAPVAKAILNHLNSFEPDLIVVELGDGIVGGYSVDS
                     ILQDFEIKRATSAFVFCASDYVGVIGGIAVLKNLGIEIDVVAGSVTDSQMGEDFVQHQ
                     FGINAGNARRDGARLFKMVGAANVEKFDYAPAA"
     gene            996..1391
                     /locus_tag="VGB68_13100"
     CDS             996..1391
                     /locus_tag="VGB68_13100"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0050224.1"
                     /translation="MKQSIAHIALVIDDYDEAIKFYTEKLNFTLLEDTPLSERKRWVL
                     VAPAGAEECCLLLAKGVGEEQRSRIGNQTGGRVFLFLKTDDFWRDYENMRQKGVGFVR
                     EPKTEDYGTVAVFQDLYGNLWDLVEFKNK"
     gene            1388..2434
                     /gene="argC"
                     /locus_tag="VGB68_13105"
     CDS             1388..2434
                     /gene="argC"
                     /locus_tag="VGB68_13105"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEY0050225.1"
                     /translation="MNEKIKIAIFGGSGYGGSELLRILLFHPNAEIALVTANEHAGKT
                     VGEVHKNLFGLTDLKFEKIDESQSALPARLEEMDCVFLALPHGQALNLAPKIPARVKI
                     IDLSGDFRIDDADVFRQFYKLEHTATALQKQFVYGLAETNREAIKQANRVANPGCFAT
                     AALLALAPVVKSGLLTGKIVVDAKTGSSGSGAKAAANTHHPQRVNSFYAYKPFTHQHV
                     PEIRQHLRAVGAFENDFVFMTHSLPVARGIFASCYVETTVNLTNEDLKKLYELYYKDS
                     FFVRLVEGSPDINWVKTTNFCDIAAHSDGKQIAVFAALDNLVKGAAGAAVQNMNLMFG
                     LDERTGLMLVGTNP"
     gene            2438..2662
                     /locus_tag="VGB68_13110"
     CDS             2438..2662
                     /locus_tag="VGB68_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050226.1"
                     /translation="MRVIYENPYFYSVLTDEKTGEYFLEVTCGRAAVYLFRIKLRKNE
                     IEKFLENENALEPLAFRIMDAPDSFLKRKI"
     gene            2676..3083
                     /locus_tag="VGB68_13115"
     CDS             2676..3083
                     /locus_tag="VGB68_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050227.1"
                     /translation="MEKEILNEETRRKLKSLSKAFLRLHKTLLDGAKVEYEAKNGRIM
                     SVNLYLQLVLDDPHFAWLRKMSSLIALIDEAVSVRRPASESEAQALFIEARLLLSFED
                     ADGEFNDKFQIALQKNSDAVLSYNDALKFTTEK"
     gene            3089..3616
                     /locus_tag="VGB68_13120"
     CDS             3089..3616
                     /locus_tag="VGB68_13120"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0050228.1"
                     /translation="MTESVEIRTASARDAETLARVGRKSFYDAFADHPKNAPGDLKIY
                     MDEAFSARTIAAELADKDVIYFIAEIGGEAVGYAKLKKNSREEATAGENPLELCRLYS
                     LNEFIGKGIGKALMRHSLDYARDNRHDFMWLGVWEYNFRAQKFYEKFGFEKCGEHVFQ
                     LGTDPQTDWLLQKKI"
     gene            3820..4986
                     /locus_tag="VGB68_13125"
     CDS             3820..4986
                     /locus_tag="VGB68_13125"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY0050229.1"
                     /translation="MKFEEIENLEEQFQLATYAKMRIAVERGRGAWIWTSDGEKYLDL
                     YGGHAVCATGHSHPRVVEALKNQAEKVLFYSNLVYSETRARAAEKLVSIAPESLTKAF
                     FCNSGTEANENAMRMARMATNREKIITFAGGFHGRTADSISATFLGKYREIGRPNVPG
                     HVCAEFGDIESVRALADEETAAIMLEPIQSMAGVREAEPSFFKHLREICDRKGIVLIF
                     DEVQTGIGRTGSWFFGGGELCDFVEPDIISLAKSLGSGVPVGACLVNQTIAGKIKIND
                     LGTTFGGGMLAMAAVPATLEAIEKDEMLENAKAVENHLRERLKEIPSVVAVRGKGCLL
                     GIEFRDKCAPVHAKLLENKIITGTSSNSNVLRLLPPLCVKKEEIDLLIETLAAK"
     gene            complement(5091..>5289)
                     /locus_tag="VGB68_13130"
     CDS             complement(5091..>5289)
                     /locus_tag="VGB68_13130"
                     /inference="COORDINATES: protein motif:HMM:NF012945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050230.1"
                     /translation="EGKASVGLQSIDRAHDFANLSGKDNAVLFYTNRYVELPLVIKGA
                     GAGADVTAAGVFADIIRAAGI"
BASE COUNT         1334 a         1283 c         1483 g         1189 t
ORIGIN      
        1 cacttacgga aatctggaat tgccgagcgg gcggctggcg aaaatcagtc gcaacgacgt
       61 tttgttcggc gttctcggca agcgccgcgc gctcaaaggt tttgtcggcg acgttcccgc
      121 gagcgtcaaa gccggcgaca ggctgcatct cctgaatatg ggcggcttaa tcggcgtctg
      181 caaaggtcat cattcttccc tttccgaggc gattgaagtc gaagttttag gaatcgcgtg
      241 tgattttgag attgcgccgc agcaggcgcg tgttttgaat atcgccgaca gggctctgaa
      301 accgtgtaat tttctcaagg aatgcgcgcc ggttgtcatc gtcgcgggaa cttgtatgaa
      361 ctccggcaaa acggtcgcgg cgactgaaat tatcaaacag gcgacgcacg ccggattaaa
      421 ggtcgcgggc gcgaaaatgt cgggcgtcgc ggctttgcgc gacacgttga atatgcagga
      481 tcacggcgcg gcggcgacgg cgagcttcct cgattgcggc ttgccgtcga cggtcgacgt
      541 cgaagattta gcgcccgtgg cgaaagcgat tttgaatcat ctcaattcgt tcgagccgga
      601 tttaatcgtc gtcgaactgg gcgacggcat tgtcggcggc tattcggtcg attcgattct
      661 gcaggatttt gaaatcaaac gggcgacttc ggctttcgtt ttttgcgctt cggattacgt
      721 cggcgttatc ggcggcatcg cggttctgaa aaatctcggc atcgagattg acgtcgtcgc
      781 cggttcggtg accgattcgc agatgggcga ggatttcgtg cagcaccagt ttggaataaa
      841 cgccggaaac gcgcggcgcg acggcgcgag gttgtttaaa atggtcggcg cggcaaatgt
      901 cgaaaaattc gattacgcgc cggcggctta aaaacgaaag aaggttattg cgtaaaaaaa
      961 ctcgtttttg atataaatag cgagcgaata aaattatgaa gcaaagcatc gcgcacatcg
     1021 ctttagtaat cgacgattac gacgaagcga taaaatttta taccgaaaaa ttaaatttta
     1081 cgcttctcga agacacgccg ctgagcgaga ggaaacgctg ggttctggtc gcgccggcgg
     1141 gcgcagaaga atgctgtctg ctgctcgcca aaggcgtcgg cgaagaacaa agaagccgca
     1201 tcggcaatca aacgggcggg cgcgtttttc tgtttttaaa aaccgacgat ttctggcgcg
     1261 attatgaaaa tatgcggcag aaaggcgtcg gttttgtgcg cgagccgaaa acggaagatt
     1321 acgggacggt cgccgtcttt caggatttat acggcaatct ctgggatttg gtcgaattta
     1381 aaaacaaatg aacgagaaaa taaaaattgc tatcttcggc ggttccggct acggcggcag
     1441 cgagcttttg cggattctgc tgtttcatcc gaacgcggaa atcgcgctcg tcacggcaaa
     1501 cgaacacgcg ggcaagacgg tcggcgaagt gcataaaaat ctgttcggct tgaccgattt
     1561 gaaattcgag aaaatagatg aaagccaaag cgcgctgccc gcgagactcg aagagatgga
     1621 ttgcgtcttt ctcgcattgc ctcacggtca ggcgttgaat ctcgcgccga aaattccggc
     1681 tcgggttaag ataatcgacc tttcgggcga ttttcgcatc gacgacgccg acgttttcag
     1741 gcaattttac aagctcgaac acacggcgac cgctttgcag aaacaattcg tttacggatt
     1801 ggcggaaacc aatcgcgaag ccatcaaaca ggcaaaccgc gtcgccaatc cgggctgttt
     1861 tgcgacggcg gctttgctgg cgctggctcc ggtggtcaaa agcggattgc tgacgggaaa
     1921 aatcgtcgtc gatgcgaaaa ccggctcgag cggttcgggc gcgaaagcgg cggcaaacac
     1981 gcatcacccg cagcgcgtga attcgtttta cgcctataag cctttcacgc accagcacgt
     2041 tccggaaatc cggcagcatc tgcgcgcggt cggcgcgttt gaaaacgatt tcgtgtttat
     2101 gacgcacagc ctgccggtcg cgcgcggcat tttcgcttcg tgctacgtgg aaacgaccgt
     2161 gaatttaacg aacgaagatt tgaaaaaact ctacgagctt tattacaaag attcgttttt
     2221 cgtccgcctg gtcgaaggct cgcccgacat caactgggtg aaaacgacaa atttttgcga
     2281 catcgcggcg cattccgacg gcaagcaaat cgcggttttc gccgcgctcg ataatctggt
     2341 caaaggcgcg gctggcgcgg ctgtgcagaa tatgaatttg atgttcgggc tggacgagcg
     2401 gacaggcttg atgctggtcg gcacgaatcc ttaaacgatg cgcgttatat acgaaaatcc
     2461 ttatttttat tcggttttga cggacgaaaa aaccggcgaa tattttctgg aagtgacctg
     2521 cgggcgggcg gcggtttacc tgtttcgcat aaaattgaga aaaaacgaaa tcgaaaaatt
     2581 tctggaaaac gaaaacgcgc ttgagccgct cgcttttcgg ataatggatg cgcccgacag
     2641 ttttctgaaa cgcaaaattt gataatctat ttgatatgga aaaggaaatt ttaaacgaag
     2701 aaacgcgccg gaaattaaaa tcactcagca aagctttttt gcggctgcat aaaaccctgc
     2761 tcgacggcgc aaaagtcgaa tacgaagcga aaaacggcag aattatgagt gttaacctgt
     2821 atcttcagct tgttctggac gacccgcatt ttgcgtggct gcgaaagatg tcttcgctga
     2881 tcgcgctgat tgacgaagcc gtctccgtcc ggcgtccggc gtcggaaagc gaagcgcagg
     2941 cgctttttat cgaagccaga ttgttattga gcttcgaaga cgcggacggc gaatttaacg
     3001 acaaatttca aatcgctctg caaaaaaatt ccgacgccgt cctgagctat aacgacgcgc
     3061 ttaaattcac gaccgaaaaa taatttttat gaccgaaagc gttgaaatca gaaccgcctc
     3121 ggcgcgcgat gccgaaacgc tggcgcgcgt cgggcggaaa agcttctacg acgcgtttgc
     3181 cgaccatccg aaaaacgcgc ccggagattt gaaaatatat atggacgaag ctttcagcgc
     3241 ccgcacgatt gccgccgaat tagccgataa agacgtaatt tattttatcg ccgaaatcgg
     3301 cggcgaagcg gtcggctacg ccaagctgaa aaaaaactcg cgcgaggagg cgacggcggg
     3361 cgaaaatccg ctggagcttt gccgtcttta ttctctgaac gaatttatcg gcaaaggcat
     3421 cggcaaggcg ttgatgcggc actctctgga ttacgcccgc gataaccggc acgattttat
     3481 gtggctcggc gtctgggaat acaattttcg cgctcagaaa ttttacgaga aattcggctt
     3541 tgaaaaatgc ggcgaacacg ttttccagct cggcacagac ccgcagaccg attggctttt
     3601 gcagaagaaa atttagccgc agagggcacg gagaacacag aggaattaac cacggatttg
     3661 cacagataaa cgcggatttt aacaggaggg gcagtataaa aagcaatgat gaacgatgaa
     3721 agcggaacgc ggaataaaaa attcatcatt catcgtttcg cgttcatcgt tttgcttcat
     3781 cctgtttgaa ttttctctgt gtcctctgtg gcaaaaatta tgaaatttga agaaatcgaa
     3841 aatctggaag agcaatttca gctcgcgact tacgcgaaga tgcggattgc cgtcgagcgc
     3901 gggcgcggcg cgtggatttg gacgagcgac ggcgaaaaat atctggattt atatggcggt
     3961 cacgcggtct gcgcgaccgg tcactcgcat ccgcgcgtgg tcgaagcctt aaaaaatcaa
     4021 gccgaaaagg ttttgtttta ttcgaacctg gtttactcgg aaacgcgcgc ccgcgccgcc
     4081 gaaaaactcg tttcgattgc gccggaaagc ctgaccaaag catttttctg caattcggga
     4141 acggaggcaa acgaaaacgc gatgcgaatg gcgcgaatgg ctacgaatcg cgaaaaaatc
     4201 atcacgtttg cgggcggctt tcacggcaga acggcggatt cgatttcggc gacgtttctg
     4261 ggaaaatatc gcgagatcgg gcgaccgaac gtgcccggtc acgtctgcgc ggagttcggg
     4321 gatattgaaa gcgtccgcgc gttagccgac gaggaaacgg cggcgattat gctcgagccg
     4381 attcagtcga tggcgggcgt gcgcgaagcc gagccttctt tttttaaaca tctgcgcgaa
     4441 atctgtgacc gaaaaggcat tgttttgatt tttgacgagg tgcagaccgg catcggcaga
     4501 accggaagct ggtttttcgg cggcggcgag ctctgtgatt ttgtcgagcc ggacattatc
     4561 tcgctcgcca aatcgctcgg cagcggcgtt ccggtcggcg cttgcctggt caatcagaca
     4621 atcgccggaa aaatcaaaat caatgatttg ggcacgactt tcggcggcgg aatgctggcg
     4681 atggcggcgg ttccggcgac tctggaagca atcgagaaag acgaaatgct cgaaaatgcc
     4741 aaagcggtcg aaaatcacct gcgcgaaaga ctgaaagaaa tcccgtcggt cgtcgccgtt
     4801 cgcggcaaag gctgtctgct cggcatcgaa ttccgcgaca aatgcgcgcc ggttcacgcg
     4861 aaactgcttg aaaacaaaat cattaccggc acctcgagca attcgaacgt tttgcgtctg
     4921 cttccgccgt tgtgcgtaaa aaaagaggaa atcgatttgt tgattgaaac tttagccgcg
     4981 aaatagcccg aaaaatcgcg aattgagagt attccgatgt tgcacggcag aaagcaactt
     5041 ttattgtttg cttgcgcctg cctgaagtaa tcatgcagcg cggtcgattt ttaaattccc
     5101 gccgcgcgga taatatccgc gaaaacgcct gccgccgtca cgtccgcgcc ggcgcccgcg
     5161 ccttttatga cgagcggcag ctcgacataa cgattcgtgt aaaacaaaac ggcgttgtcc
     5221 ttgcccgaaa gattggcgaa atcgtgagcc ctgtcgatgc tctgcaaacc gaccgaggct
     5281 ttgccttcg
//
LOCUS       DASWNE010000672         3397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_514984, whole genome shotgun sequence.
ACCESSION   DASWNE010000672 DASWNE010000000
VERSION     DASWNE010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514984"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="VGB68_13135"
     CDS             complement(<1..847)
                     /locus_tag="VGB68_13135"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEY0050231.1"
                     /translation="MKKDFNNNSFIRPAASSSGQFPARQPVSRRFLAFFVFAILLFQQ
                     FAFFVPASAETFTLLAARAAVAVSDDIVISQVYGGGGNAGSVYRNDFIEIFNRGTGAI
                     SVTGWSVQYASATGSSWQKTDLTGTIPPGGYYLIQQAAGTGGTTNLPTPNATGNIAMS
                     GTAGKVALVTNTTLLTCGGATNNCFPNAAIKDFLGYGTTANNYEGAPTANLSNTTAAI
                     RNGNGCTDTDNNSTDFTLAAATPRNSASPNAPCSTSTNPSGTGTANPASVVAGGSTLL
                     TVNVTP"
     gene            complement(1597..1782)
                     /locus_tag="VGB68_13140"
     CDS             complement(1597..1782)
                     /locus_tag="VGB68_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050232.1"
                     /translation="MESFRVKQGNPALNEKVLRRLYIAEHKTAADIAEMFGYAMPSIK
                     KMLWKLGIRKRDLKKKV"
     gene            complement(2223..2570)
                     /locus_tag="VGB68_13145"
     CDS             complement(2223..2570)
                     /locus_tag="VGB68_13145"
                     /inference="COORDINATES: protein motif:HMM:NF024001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3761 domain-containing protein"
                     /protein_id="HEY0050233.1"
                     /translation="MRKSVGQNGDALETLSAGASVEVIKQNGAWFLVKSGNREGWIHG
                     NAIRLAKTNNGGQTADTTTKPGSYTERRSYSTPEPRRNTSGASALCRDGTLSYSRNRR
                     GTCSHHGGVSQWY"
     gene            2587..2931
                     /locus_tag="VGB68_13150"
     CDS             2587..2931
                     /locus_tag="VGB68_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050234.1"
                     /translation="MTVAGSDNEFVFAAVGETVGAASDEFGVEAGAGVCNNGTGEAAF
                     EFVREIIALFEFCGLVFSTIEPITPMKPQIPNMAVTAIINPTGVLMPFLAGSVAERLK
                     ADAQLTQKRASG"
     gene            complement(3015..>3397)
                     /locus_tag="VGB68_13155"
     CDS             complement(3015..>3397)
                     /locus_tag="VGB68_13155"
                     /inference="COORDINATES: protein motif:HMM:NF024001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3761 domain-containing protein"
                     /protein_id="HEY0050235.1"
                     /translation="VARETRRAEALETPVPIYRSTPGPDYSQRDETSSLTTDESSSST
                     PTALCVDGTYSYSANRRGTCSWHGGVAEWLNSTPSSSSPETYPRSSSGDSTYRPKTVN
                     VRGYTRRDGTYVAPYTRSAPRRRN"
BASE COUNT          741 a          839 c          902 g          915 t
ORIGIN      
        1 ccggcgtaac gttaacggtt aaaagcgtgc tcccgcccgc gactaccgac gccgggttgg
       61 cagtgccggt tcccgatgga tttgtcgagg tgctgcacgg agcgttgggc gacgcactgt
      121 tgcgcggcgt cgccgcggca agggtaaaat cggtcgaatt attgtcggta tcggtacagc
      181 cgtttccgtt gcgaatggcg gcagtcgtgt tgcttaagtt agccgtcggt gcgccttcat
      241 agttattggc ggtcgttccg taaccaagaa aatctttgat cgccgcgttg ggaaaacagt
      301 tattggtcgc gccgccgcag gtcaaaagag tggtattggt gaccagcgcg actttgcctg
      361 ccgtcccgct catggcgata ttgcccgtcg cgttgggagt cggcagattg gttgttcctc
      421 cagttccggc ggcctgctga atcaaatagt agccgccggg cgggatcgtc cccgtcaagt
      481 cggttttctg ccatgacgag ccggtcgccg aagcatactg caccgaccag ccggtcacgc
      541 tgatcgcgcc ggttccgcgg ttaaagattt cgataaagtc gttacgataa acggagcccg
      601 cattgccgcc gccgccgtac acctggctaa taacaatatc gtcggagaca gcgactgccg
      661 cccgcgccgc gagcagcgtg aaagtttcgg cgctggccgg aacgaaaaac gcgaattgct
      721 gaaacagtaa aattgcgaag acgaaaaaag ccaggaagcg gcgcgaaacc ggttgccggg
      781 cgggaaattg accggaggag gatgccgccg gacggataaa cgaattatta ttaaaatctt
      841 tcttcatata gggaatacct taatctcctg aaataaacga accgcttttg aataatctgc
      901 agttttcaaa atcgcgcgag cgtcggtctg tgcttattct gtcaggcaga gagaatccgc
      961 gcgcttaaaa gaattcgaaa atattttgcc aaatgatgtg ggtagtttta tccagaccct
     1021 tagccagccc tcgggtgaaa gttctgttgg tcggataaaa ctgaataata tctcaaaccg
     1081 ggcagaaaag taagcgggca gatgtaaaat taagaagttt gtcacattct ccgaaaaaat
     1141 ttaagcgtct gcgagattct accggctcgg cgggctatcc gaaatgcctg ctatcacctt
     1201 gcaaattatt tatatttaag tatcatactt tttaattgcg ttagaaattc gagagcatcc
     1261 gttttgaatt tcggataacc tcgacagttc atttattgtt ttcaataaat gaacggaaaa
     1321 gagtcggtat catccggctt tttccgtttg acgagtagtt ttacagcggc ttcgcactta
     1381 tttggagttg aagttagttc gcctgcgggt tgccatcctt aatcttattt tgttatattg
     1441 cggacagccg attgagaaaa tcaattggtc ggataaacag aatagagcgg caagtttacg
     1501 ggcgagccgc cctttttttg ttccgctatt gaaactaaaa tatttaaacc cgccttttcg
     1561 cctgtcagaa atataaaggg ttccagcctg tttcgtttac acttttttct ttaaatcgcg
     1621 ctttctgatg ccaagtttcc agagcatttt ctttatactc ggcatcgcat acccgaacat
     1681 ttcggcaatg tcggctgccg ttttatgctc ggcgatatag agccgtcgca ataccttttc
     1741 gtttagcgcg ggattgcctt gcttgacgcg aaatgattcc actcttgttc gcagaggtat
     1801 cccatatctg attaggttgc ttttgaccgt cgacgggctg acgctgaatc gcgccgccgc
     1861 ttgccgcaga gtcagctttt cggtggaata taatttttcc agttcatctc tggataaagt
     1921 tttaagttta tctttccact ggcgagcacg ttggcgctta aggctcggtg atttcgtata
     1981 tcggcgtatc gggaaaccga actttttgaa caattgccgg actctttcac gggagaggtt
     2041 aaattcctgc ccgactttct gcagcgaaca atctttttca taccgggcgt acatgcgcgc
     2101 cacttcttct ctggtttttg gtttgtagcc tagcggcgcc ttgctcgtat ttaccacgct
     2161 tgaaaattaa tgggaaagcc cgtgccccgg ctcgccgatt ttagtaatct gatttctttc
     2221 tcttaatacc actgggagac gccgccgtga tgagagcagg tgccgcgccg gtttcgactg
     2281 taactaagcg tgccgtcccg gcacagagcg gaggcgcccg aagtgtttct acgcggttct
     2341 ggggtcgaat agctgcgccg ctctgtataa ctgcctggtt tggtggtcgt atcggcagtt
     2401 tgcccgccgt tattggtttt cgccagacga atcgcgttgc cgtgtatcca gccttcccga
     2461 ttgcccgatt ttaccagaaa ccaggcgccg ttttgtttta taacttcgac gctcgcgccg
     2521 gctgaaagcg tttccagggc gtcgccgttt tgcccgacgg atttacgcaa gacagcgttt
     2581 tcggctatga cggtagcagg cagcgataac gaatttgttt tcgccgcggt cggagaaacg
     2641 gtcggagcgg cgagcgacga gttcggtgtt gaagcaggtg ccggagtttg caataacggg
     2701 accggagaag cagcatttga gtttgttcgt gaaattatcg cgctattcga attttgcggc
     2761 ttggttttct ctactattga accaattacg ccgatgaaac cgcagattcc gaacatggcg
     2821 gtcacggcga ttataaaccc cacaggcgtg ctgatgcctt ttctcgcggg cagcgttgcg
     2881 gaaagactaa aagccgacgc gcaattaacg caaaagcggg catccggttg attttgcgcc
     2941 cggcagcgcg agcaggtttt cattcgttat tttcttattt tccttcaatt caacttcttt
     3001 ttaatctatt tcccttaatt ccttctgcgc ggcgcgctgc gggtataagg cgcgacataa
     3061 gtgccgtccc tgcgcgtgta gcctctcacg ttaaccgttt tcgggcgata agttgaatcg
     3121 cctgaagatg atcgcggata agtttctggg gaactcgaag aaggagtgga atttaaccac
     3181 tcggctactc cgccgtgcca cgaacaggtt ccgcgccggt tcgcgctgta actatatgtt
     3241 ccgtcgacgc acagagcggt cggcgtgcta ctgctgcttt catcggtcgt aagggacgac
     3301 gtttcatcgc gctgtgaata atcaggtccc ggagtgcttc ggtatattgg aacaggcgtt
     3361 tctaaagctt ccgcccgccg cgtctcgcgc gcaaccg
//
LOCUS       DASWNE010000673         4488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_92608, whole genome shotgun sequence.
ACCESSION   DASWNE010000673 DASWNE010000000
VERSION     DASWNE010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92608"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1113)
                     /gene="murG"
                     /locus_tag="VGB68_13160"
     CDS             complement(34..1113)
                     /gene="murG"
                     /locus_tag="VGB68_13160"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEY0050236.1"
                     /translation="MKVLIAAGGTGGHIYPGIAVAKEIMRRSAGASVKFVGTARGLET
                     RLVPDSGFELLTIDSAGLKNVGFAGKIKGLMLLPKSFLEARHLIKNFKPDVVAGAGGY
                     VSGPVLLMASLMRVPTLVMDSNALPGFTNRRLAPFVTKAALTFEEAVPFFGKKGIVTG
                     NPVRREFFEIPPKARNAQKFSLLIFGGSQGARAINNALVEALEHLRDYKKRLEITHQT
                     GETDFEKIKQGYADAGWSEAEVSAYIKNMPEHFEKNDLIICRAGATSCAELAAAGKAS
                     IMIPLPTAADDHQRKNAEALEKAGAARMILQKDLTGAGLAKQIIELIDAPARITEMEA
                     SARKLAKADAAEVTVDLIEELARRN"
     gene            complement(1238..2428)
                     /gene="ftsW"
                     /locus_tag="VGB68_13165"
     CDS             complement(1238..2428)
                     /gene="ftsW"
                     /locus_tag="VGB68_13165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEY0050237.1"
                     /translation="MAKKLRIDWFLFAIAATLALFGAVMVYSASAMISLQETKNNPEG
                     ASQFLYFYKQIGFTAAGLLVMYLATKFNYRWLQNERVVYGLLIVTAILLVAVFAFPPI
                     NGARRWIRFPGASFQPSELAKITLPVFLAYFLTKNEKNVGSLRETALWCVAVFGILSG
                     LILFEPDLGTVIVLAAIFVAVYFAAGARLLHIAALGAGCVSIFALALITSPFRRARVL
                     AFLNPWENSDGAAYQVIQGLYAIGSGGIFGEGFAKGEQKLFYLPYPHSDFIFAVVGEE
                     LGLIGTLAVVLAFGLLLWRGTRAALLAPERFGMLLGIGIITGIIVQALFNISVVISIL
                     PAKGIPLPFISYGGTSIVTTLFAVGLLLNLSQHAGFVSMEKNEPEQPRPKKRQKVSRT
                     RATA"
     gene            complement(2421..3788)
                     /gene="murD"
                     /locus_tag="VGB68_13170"
     CDS             complement(2421..3788)
                     /gene="murD"
                     /locus_tag="VGB68_13170"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEY0050238.1"
                     /translation="MVLGAGKSGIASAKFLAARGAVVALHDRKPIAEWTEEARSLKEQ
                     KVGLLDGQIPSWLLDQIDLVVISPGIPTKTVPARYVDRKDGEVIGEVELAYRFLKGRI
                     VGITGSNGKTTTTTLIGELLKNAGIKTRVGGNIGTPLLNLTETADEETWTVAELSSFQ
                     LETIKDFRPNVGLCLNVTPNHMDRYDFFTDYAAAKHRLFMNQTAEDLAILNADDAVTA
                     SWASGLKANVVLFSVKKELEEGLFLRGRDLVCRANGREKILATRDEIFLRGLHNVENV
                     LAGLAAGLACGASPDSMRETVASFKGVEHRIEFVADVRGVKFYNDSKATSVDATLKAL
                     EAFSEEAGKTILILGGLGKKAPYAPLIPLIEKSVRKLILIGEDAYNIDNQLSDFVETD
                     FADDLQQAVLKGFQSAEPGDNVLLAPACASFDWFSSFEQRGTIFKKEVGHLKSKIEKK
                     LRQNG"
     gene            complement(3949..>4488)
                     /gene="mraY"
                     /locus_tag="VGB68_13175"
     CDS             complement(3949..>4488)
                     /gene="mraY"
                     /locus_tag="VGB68_13175"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEY0050239.1"
                     /translation="FVLFGSSNAVNLTDGLDGLATSVTFIAMSALTGLTYLASDARWA
                     ERLDLTHKPEAAELTIFCGAMVGASLGFLWYNAPKAEVFMGDVGSLAIGGALGTVAIL
                     TKQEFLLPFIGGVFILEALSVMIQVVSFKLTGKRVFKMSPLHHHFEFKGWDEPKIVFR
                     FVIAAILFALLSLSTLKLR"
BASE COUNT         1117 a         1329 c         1019 g         1023 t
ORIGIN      
        1 tttttcgaaa aatctgtaat ctgtaatctg caatcaattt ctccttgcta actcctctat
       61 taaatccact gtgacctccg ccgcatcggc tttcgccaat tttcgggcgg aagcttccat
      121 ttccgtaatc cgcgcgggcg catcaatcaa ttcgataatc tgttttgcca acccagcgcc
      181 cgtcaaatcc ttctgtaaaa tcattcgcgc cgcgccggct ttttccaatg cttcggcgtt
      241 tttgcgctgg tggtcgtcgg cggcggtcgg cagcggaatc ataatcgaag cctttcccgc
      301 cgccgccagc tccgcgcagc tcgttgcgcc cgcccggcaa attatcaaat cgtttttttc
      361 aaaatgttcg ggcatatttt tgatgtatgc cgaaacttcc gcttcgctcc aaccggcgtc
      421 ggcgtagccc tgctttatct tttcaaaatc ggtttcgcca gtctgatgcg tgatttcgag
      481 acgttttttg taatcccgca aatgctctaa agcctcgacc agcgcgttgt taatcgcccg
      541 cgcgccctgc gagccgccaa aaatcaaaag cgaaaatttt tgggcgtttc tggctttcgg
      601 cggaatttca aaaaactccc ggcgcaccgg atttccggtc acgattccct tttttccaaa
      661 aaacggaacg gcttcttcaa acgtcaaagc ggctttcgtc acaaacggcg cgagccgtcg
      721 attggtaaaa ccgggcagcg cgttggaatc catcaccagc gtcggaacgc gcatcaggct
      781 cgccatcaaa agaaccggtc ccgaaacata accgcccgcg ccggcgacca cgtccggctt
      841 aaaattcttt atcaaatgcc gcgcttccag aaaacttttc ggcaaaagca tcaagccttt
      901 gatttttccc gcaaaaccga cgtttttcag cccggcgctg tcgattgtca aaagctcaaa
      961 accgctgtcg ggcaccagcc gcgtctccag cccgcgcgcc gtgccgacaa atttcacgga
     1021 cgctccggcg ctgcgccgca taatctcttt ggcgaccgcg attcccggat aaatatgtcc
     1081 gcccgttcct ccggcagcga ttagaacttt catacattta ttcaaacagg agataaatta
     1141 aaacgatgaa ccgggaacga tgaacgatga attctttatt ccgcgttccg cattcatagt
     1201 tccgcgttcc gcttcatcct gtttgaattc ctttttatca agctgtcgcc cgcgttctgg
     1261 aaaccttttg ccgtttcttc ggtctcggct gttccggttc atttttctcc atcgaaacaa
     1321 aaccggcgtg ctgcgacaaa ttcaataaaa gcccgaccgc gaaaagcgtc gtcacaatcg
     1381 aagttccgcc gtaggaaata aacggcagcg gaattccctt ggcgggcaaa atcgaaatca
     1441 cgacgctgat attaaaaagc gcctgcacga taattccggt aataatgccg atgccgagca
     1501 gcattccgaa tctttccggc gcgagcagag ccgcgcgcgt tccgcgccac aaaagaagcc
     1561 cgaatgccaa aacgaccgcc agcgttccga ttaagccgag ctcttcgccg acgacggcga
     1621 aaataaaatc cgaatgcgga tacggcagat aaaaaagctt ctgctcgcct ttggcaaagc
     1681 cttcgccgaa aatcccgccc gagccgatgg cgtaaagtcc ctgaatcacc tgataagccg
     1741 cgccgtcgga gttttcccag gggttcagaa aagccagcac gcgcgcgcgc cgaaacggcg
     1801 aagtaatcaa agcgagcgcg aaaattgaaa cgcagcccgc gcccagcgcg gcgatatgca
     1861 gaaggcgcgc gcccgcggcg aaataaaccg ccacgaaaat cgccgccagc acgataaccg
     1921 ttcccaaatc cggctcaaac aaaatcaaac cgctcaaaat gccgaaaacc gcgacgcacc
     1981 acaaagccgt ctcgcgcagg ctgccgacgt ttttctcgtt tttcgtcaga aaataagcca
     2041 gaaaaaccgg cagagtaatt ttcgccagct ccgacggctg aaacgacgcg cccggaaaac
     2101 gaatccagcg ccgcgcgccg tttatcggcg gaaaggcgaa aaccgcgacg agcaaaatcg
     2161 ccgtcacgat taaaagcccg taaactaccc tctcgttttg cagccagcga taattgaatt
     2221 tcgtcgccag atacatcacc agcagaccgg ctgccgtaaa accgatttgc ttgtaaaaat
     2281 acaaaaactg gctcgcgcct tccggattat ttttggtttc ctgcagggaa atcatcgccg
     2341 acgccgaata gaccatcacc gcgccaaaca gcgcgagcgt cgcggcaatc gcgaataaaa
     2401 accaatctat gcggagtttc ttagccattc tgtcttaatt ttttctcaat cttcgacttt
     2461 aagtgtccga cttccttttt gaaaatcgtt ccgcgctgct cgaaactgct aaaccaatca
     2521 aaactcgcgc aggcgggcgc aagcaaaaca ttgtcgcccg gttcggctga ttgaaaacct
     2581 ttcagcaccg cctgctgcaa atcgtcggcg aaatcggttt caacgaaatc gcttaattga
     2641 ttgtcaatat tgtaggcatc ctcaccaatt aaaattagtt tgcgaaccga tttctcaatt
     2701 aatggaatca acggcgcata gggcgctttt ttgcccagac cgccgagaat taaaatcgtc
     2761 tttccggctt cttccgaaaa cgcttcgagc gcctttaaag tcgcgtcgac cgaggttgct
     2821 ttcgaatcgt tataaaattt cacgcctctc acgtcggcga caaactcgat gcgatgctcg
     2881 acgcctttaa aactcgccac ggtctcgcgc atcgaatccg gcgacgcgcc gcaagccaag
     2941 cccgccgcca agcccgccag aacattctcg acattgtgca gaccgcgcag aaaaatctcg
     3001 tcgcgcgtcg ccagaatttt ttccctgccg tttgctcggc agaccaaatc gcgcccgcgc
     3061 aggaacaagc cttcttccag ctcttttttg acggaaaaca aaacgacatt cgctttcaaa
     3121 ccgcttgccc agctcgccgt caccgcgtcg tcggcgttta aaatcgccaa atcttccgcc
     3181 gtctgattca taaacagacg atgtttcgcc gccgcgtaat cggtaaaaaa atcgtatctg
     3241 tccatatgat tcggcgtgac gtttagacac aagccgacat tcgggcgaaa atctttaatg
     3301 gtctcgagct gaaagctcga aagctcggct accgtccagg tttcctcgtc ggcagtttcg
     3361 gtcagattca aaagcggcgt tccgatgttt ccgccgaccc gcgttttaat tccggcgttt
     3421 ttcagcagct cgccgattaa agtcgtcgtc gtggttttac cgttcgagcc cgtgattccg
     3481 acgattctgc ctttcaaaaa tctgtacgcc agttcgactt cgccgatgac ttcgccgtct
     3541 ttgcggtcaa cgtagcgcgc cgggacggtt ttcgtcggaa tgcccgggct gatgacgacc
     3601 aaatcaatct ggtctaaaag ccaggacgga atctgcccgt ccagcagacc gactttttgt
     3661 tctttcaaag accgcgcttc ttccgtccat tcggcaatcg gttttctatc gtgcagggcg
     3721 acgaccgcgc cgcgcgccgc cagaaatttg gcggaagcga tgccggattt tcccgcgccg
     3781 agaaccaaag ttttttttcc ttgaagctcc actttccaaa caaagattca aacaggatga
     3841 acaggataga taggatgaac tattgataat tcaattttta aaattcaatc aggttgataa
     3901 gctaatgaaa taatcctgtc tatcctgtcc atcctgtttg aatctgcctt atcgcaattt
     3961 cagcgtcgat aaactcaaaa gcgcaaaaag tatcgccgca atgacgaagc ggaaaacgat
     4021 tttcggctcg tcccagcctt taaactcgaa gtgatgatgc agcggcgaca ttttgaaaac
     4081 gcgttttccc gtcagcttaa acgacacgac ctgaatcatc accgacagcg cttccagaat
     4141 aaacacgccg ccgataaacg gaagcagaaa ctcctgtttc gtcagaatcg ccaccgtccc
     4201 gagcgcgccg ccgatggcga gacttccgac gtcgcccata aaaacttcgg ctttcggcgc
     4261 gttataccat aaaaatccca aagaagcgcc gaccatcgcg ccgcaaaaaa tcgtcaattc
     4321 cgccgcttcc ggcttgtgcg tcaaatcgag ccgctcagcc cagcgcgcgt cgctggcgag
     4381 atacgtcaaa cccgttaacg ccgacatcgc gataaacgtg acgctcgttg ccaaaccgtc
     4441 caagccgtcc gtcagattga ccgcgttcga cgagccgaaa aggacaaa
//
LOCUS       DASWNE010000674         1736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_554070, whole genome shotgun sequence.
ACCESSION   DASWNE010000674 DASWNE010000000
VERSION     DASWNE010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554070"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="VGB68_13180"
     CDS             <1..834
                     /locus_tag="VGB68_13180"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0050240.1"
                     /translation="AQKMVDLKPNTASYSRISYLRSLHGDTKGAIEAMKMAVESASPQ
                     NPENIAWSRVHLGDELMNDGRLTEAEHEYDYALYLFPEYHPALAAKARARYAAGDIDN
                     AIIFYKRAAERVPLPEYIAPLGDLYAKLNRADEAKQQYEQVEFIEKMGGAAGETYSRQ
                     LALFWADHDVRLDEALAAAERERATRSDIYTFDVLAWCLYKKGRFEEAKTAIGEALRL
                     NTRDPRLLYHAGMISLATGDNQKGSDYLKQALAINPSFDILQADVAKEKLNALKNATA
                     F"
     gene            924..1403
                     /locus_tag="VGB68_13185"
     CDS             924..1403
                     /locus_tag="VGB68_13185"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY0050241.1"
                     /translation="MFRKKITIAPIIFLILTAIVLSAGCAKQTESQAAQTGSVNKAPA
                     AANNNNISATPPNAPPVNNVSTATTVAADNRKPAPAVKEPTPQIGSGGDDMSLFTQVR
                     AALSPDKELFNAVIIEIKEGNATLNGKVSSEAQKAKAAQLVQSVKGIKSVKNNLRVS"
     gene            1468..>1736
                     /locus_tag="VGB68_13190"
     CDS             1468..>1736
                     /locus_tag="VGB68_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050242.1"
                     /translation="MRKIILAIMPALLLMTLASLSARAQGTVKKETEKTTTLDAWRQA
                     LPDGESSNTPPVVAMDESKDNVESEETPAQIEKRILDLETRLMEA"
BASE COUNT          481 a          459 c          456 g          340 t
ORIGIN      
        1 gcgcagaaaa tggtcgattt aaaaccgaat acggcttctt attcgagaat ctcctacctg
       61 cgctcgcttc acggcgatac gaaaggcgca atcgaggcga tgaaaatggc ggtcgaatcc
      121 gccagcccgc aaaatcccga aaatatcgcg tggagccgcg ttcatctggg cgatgaattg
      181 atgaacgacg gtcgattgac cgaggctgaa cacgaatacg attacgctct gtatctgttt
      241 cccgagtatc atccggcgct tgccgccaaa gcccgcgccc gttatgcggc gggcgatatc
      301 gacaacgcaa taatatttta taaacgcgcc gccgagcgcg tgccgctgcc cgaatatatc
      361 gctccgctcg gcgatttata cgcgaagctg aatcgcgccg acgaggcgaa acaacaatac
      421 gagcaggtgg agtttatcga aaaaatgggc ggcgcggcag gcgagactta ctctcgtcaa
      481 ttagctttat tctgggctga ccacgacgtg cgtctggacg aagccttagc cgccgccgag
      541 cgcgagcgcg ccacgcgcag cgatatttat acctttgacg ttctcgcctg gtgcctctac
      601 aaaaaaggac gatttgaaga agcgaagacc gccatcggcg aagccctgcg cctgaacacg
      661 cgcgaccctc gcctgcttta tcacgcgggt atgatttcgc tggcgacggg cgataatcaa
      721 aagggctccg actatctgaa acaggcttta gccattaacc cgtctttcga cattcttcag
      781 gcggatgtcg caaaggaaaa attgaacgcc ctcaaaaacg caacggcgtt ttgacggcgt
      841 tcccgaacaa aattttaacg gcggtcggat tcatataaat tcccgccgct ttcaaaagaa
      901 tttatttttt gagaatgaac acgatgttta gaaaaaaaat cacgatagcg ccaatcattt
      961 ttttgatttt gacggcaatt gttttgtcgg cgggctgcgc caaacagacc gagtcgcagg
     1021 cggctcagac cggcagcgta aataaagcgc ccgctgccgc aaataacaac aatatttccg
     1081 ctactccgcc gaacgcgccg ccggtcaata atgtttctac cgcgacgacc gtcgccgcgg
     1141 ataatagaaa gcccgcgcca gccgtcaagg aaccgacgcc gcaaatcggc agcggcggcg
     1201 acgatatgtc tttattcacg caggtgcgag ccgctctgag cccggataaa gaattgttta
     1261 atgcggtcat tattgaaatc aaggaaggaa acgcgacctt aaacggcaag gtttcgagcg
     1321 aagcccagaa ggcgaaagcc gcgcagctcg tccagagcgt caaaggaatt aaaagcgtca
     1381 aaaataattt gcgcgtttcg taataagtaa agaaacggct cgcctgatta taaaagagaa
     1441 gccgaaacag ggaaggacaa caaaccgatg cgaaaaatca ttttagctat aatgcccgcg
     1501 cttttgttaa tgacattggc gagcctcagc gcccgcgcgc agggcacggt gaaaaaggaa
     1561 accgagaaaa ccacgacgct cgacgcgtgg cgtcaggcgc tgccggacgg cgagtcctcc
     1621 aacacgccgc ccgtcgttgc gatggacgaa tcgaaagaca atgtggaaag cgaagaaact
     1681 ccggcgcaga ttgaaaaaag aattctcgac ctcgaaacaa gattgatgga agcctt
//
LOCUS       DASWNE010000675         6450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_334775, whole genome shotgun sequence.
ACCESSION   DASWNE010000675 DASWNE010000000
VERSION     DASWNE010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6450
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334775"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VGB68_13195"
     CDS             complement(<1..472)
                     /locus_tag="VGB68_13195"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY0050243.1"
                     /translation="MKSVFGRLLFSAILLSFLMFTFAVSGFAQDLDDVTISGKVADTN
                     GAAIVGATVTATQIEKNIERTIVTDDEGRYRLVDLQPGTYKVRATSQGFGTKEQIDLV
                     TIAGQNVQLDFSLAPAGLQVEQTVTVGGDDAPVVDTTRTVVGGTVTEREIEELPN"
     gene            complement(640..1047)
                     /locus_tag="VGB68_13200"
     CDS             complement(640..1047)
                     /locus_tag="VGB68_13200"
                     /inference="COORDINATES: protein motif:HMM:NF024979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0050244.1"
                     /translation="MKKKVKTYTISAVAELFDVHPQTLRLYEREGLLKPSRSEGNTRL
                     YTDTDLERLEVILSLTRDLGVNLAGVEIILNMREKMDAMQHEFERFFEYLRTHANDFP
                     RPSEQLSESEALIPISRLVLMQQTRRKGNGGDE"
     gene            complement(1171..2352)
                     /gene="dnaJ"
                     /locus_tag="VGB68_13205"
     CDS             complement(1171..2352)
                     /gene="dnaJ"
                     /locus_tag="VGB68_13205"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEY0050245.1"
                     /translation="MANKDYYGILGIKKDAKADEIKKAYRRLARKHHPDVNPNDKASE
                     EKFKEVQEAYDVLSDEKKRKVYDRFGYYNDNLDPDSPFGAGAGAGSGAGGFDFSGFTW
                     DASSGSSGGGGGTGGTSFRDIFSDLFGGSGSRAQTQPEPPRPLPKKGKDIEIPLALSF
                     EESVTGLTTNLTVERSEQCSRCQGAGDTGGPVVVCSTCHGSGQVQKQGGRLQFSQSCP
                     DCEGTGRRRQPCSQCHGKGVTPKTEQVKVRIPAGVDTGSRVRIPKKGHGGRLGAEPGD
                     LFILTNVGKHRFFTRKGDNIYVTVPITVPEAALGAKIEVPTVEGRAQLKIPAGTQSGQ
                     KFRLRERGVPSLRNPQLRGDQFVEVQIALPRIISEESKEILREFEKANSENPRKAMGL
                     E"
     gene            complement(2715..2969)
                     /locus_tag="VGB68_13210"
     CDS             complement(2715..2969)
                     /locus_tag="VGB68_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050246.1"
                     /translation="MSVFIELDEKLVAEIDAVAKDFNKNRVQYINESLRKTLHDDLRK
                     RKLSEEEIGKMYQEAYEKFPVQSDEFEVEDEQMEEFWKQV"
     gene            complement(3048..4988)
                     /gene="dnaK"
                     /locus_tag="VGB68_13215"
     CDS             complement(3048..4988)
                     /gene="dnaK"
                     /locus_tag="VGB68_13215"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEY0050247.1"
                     /translation="MSKIIGIDLGTTNSVVAVMEGGEPVVITNEEGGRTTPSVVAFTK
                     DGNRLVGQVAKRQAVTNPENTLYSIKRFMGRKYEEVQGEIKQVPFKVERAGNGDVRID
                     AQGKQYSPPEVSAMVLQKLKKAAEDYLGAKVDKAVITVPAYFNDAQRQATKDAGKIAG
                     LEVMRIVNEPTAAALAYGLDKKKDETIAVFDFGGGTFDISVLEVGEGVVEVKSTNGDT
                     HLGGDDVDEALIKWIIDEFKKDQGIDLTADKMALQRLKESAEKAKVELSSAMETEINL
                     PFITADQSGPKHLVMKMTRTKFEALTEPLLKRLMPPVEQALKDAGVEKGQIDEIVLVG
                     GSTRIPKVQEMVKEFFGKEPNKSVNPDEVVALGAAVQAAVLGGEKTDILLLDVTPLSL
                     GIETLGGVMTQMIGRNTTIPTRKSEIFSTASDNQTSVEVHVLQGERPMAADNKTLGKF
                     HLVGIPPAPRGVPQVEVTFDIDANGIVNVSAKDVGTGREQKITITASSGLSKEEIDKM
                     MKDAESHAGEDTKRKETIEARNRLDGLVYSVEKSFGENKDKLDAGAAGEIEAAISESK
                     TALGAEDADAMNNAFNRLQTASHKLAEVLYSQAGSAQTGGAETGGEQASSASAGAGET
                     SSTTSDDNVIDAEYVDVDEEKK"
     gene            complement(5521..>6450)
                     /locus_tag="VGB68_13220"
     CDS             complement(5521..>6450)
                     /locus_tag="VGB68_13220"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0050248.1"
                     /translation="NSQTNLLLDAQGNVYVAGDSVIPDQSINLVVFKLEPVAQLRAVP
                     FDFDGDKKADVAVFRPETGSWYVLKSTDGSFTAAQWGISTDKLVPADYDGDGKTDFGV
                     YRDGTWYVQKSADGNYFASRFGIASDKPVPSDFDNDGRADLSVFREGVWYSLQSSDGA
                     FKGVQFGSANDVPLPSDYDSNRRSDIAVFRGGSWYVKYQNELPLASTQFGISTDKPVP
                     ADYDGDKKTDYAVFRNGDWYVWQSSTNSMRVQHWGASGDVPVPADYDGDKRTDFAIFR
                     AGVWYILKSSDNQYYAVQWGLPSDKPVQAAFAQ"
BASE COUNT         1443 a         1766 c         1542 g         1699 t
ORIGIN      
        1 cgttcggaag ctcttcgatt tcgcgttcgg tgacggttcc gccgacgacc gtccgcgtgg
       61 tatcgacaac gggcgcatcg tcgccgccga cggtcacggt ttgctcgacc tgcaagccgg
      121 ctggcgcgag cgaaaaatcg agctggacgt tttgtccggc aatcgtcacc agatcaatct
      181 gctcttttgt gccgaagccc tgcgacgtcg ctctgacttt gtaagttccc ggctgcaaat
      241 cgacgaggcg gtaacgacct tcgtcgtcgg tgacgattgt gcgctcgata tttttttcaa
      301 tttgcgtggc ggtaaccgtc gcgccgacaa tcgccgcacc gtttgtatcg gcgactttac
      361 cgctaatcgt cacatcgtcg aggtcctggg caaaaccgct gaccgcaaat gtaaacatta
      421 aaaaactcag caagatagcg gaaaacaaaa gtcttccaaa tacacttttc attaaagctc
      481 tcctagaatt tttaaatagg tttcagccgc caatctgggc tgaaacgaaa tggacacctt
      541 ttaagggatt gtgaaagggt ctattgtgtc gcaagaaggc aggaaaagta aaggagaaaa
      601 cctgaatttc cggattttaa gggttaaatg aaagtaaaat tattcgtcgc cgccgtttcc
      661 tttccggcga gtttgctgca tcaggacgag gcgcgaaatc ggaatcaggg cttcggattc
      721 ggaaagctgt tcggacggac gcggaaaatc gttggcgtgc gtccgcagat attcaaaaaa
      781 gcgctcgaat tcgtgctgca tcgcgtccat tttttcgcgc atattgagga tgatttcgac
      841 gcccgccaga ttgacgccca aatcgcgcgt cagcgagaga ataacttcca gacgttcgag
      901 gtcggtatcg gtgtaaagcc gcgtgttgcc ttccgagcgc gaaggtttca gcaagccctc
      961 gcgttcataa agccgcaaag tctgcgggtg cacgtcaaaa agctcggcaa cggcgctgat
     1021 tgtgtatgtc ttaaccttct tcttcatact tttatgagtt gactgaaagt tgactgagtt
     1081 gactgaaagc ttgcggagtt aaaaactcag tcaactcggt taactcagcc aactcagtca
     1141 actcggttaa ctcagtcaac tttcagtcaa ctattccaac cccatcgcct tgcgcggatt
     1201 ttcggaattg gctttttcaa actcgcgcaa aatttccttg gattcttccg aaataattcg
     1261 cggcagagct atctgcactt cgacaaactg gtcgccgcgc aattgcggat ttctcaaaga
     1321 cggaacgccg cgctcgcgca ggcgaaactt ttgtcccgac tgcgttcccg ccggaatttt
     1381 caattgcgcc ctgccttcga cggtcggcac ttcgattttc gcgccgagcg ccgcctccgg
     1441 cacggttatc ggcacagtca cataaatatt atcgcccttg cgcgtaaaaa accggtgctt
     1501 gccgacgttc gttaggataa acaaatcgcc cggctcggcg cccaatcttc cgccgtgacc
     1561 ttttttcgga attcgcacgc gcgagcctgt atcgacgccc gccggaatgc gaactttcac
     1621 ctgctcggtt ttcggcgtga cgcctttgcc gtgacattgc gaacaaggct gacgtcgtct
     1681 gccggttcct tcgcaatcgg ggcagctctg cgaaaactgc aatcgtccgc cctgtttctg
     1741 cacctgcccg cttccgtgac aggtcgagca aaccacgacc ggaccgcccg tatcgcctgc
     1801 gccctggcag cgcgagcatt gctcggaacg ctcgaccgtc agattcgtcg tcagtcccgt
     1861 gaccgattct tcaaacgaca gagccagcgg aatttcaata tctttgcctt ttttcggcag
     1921 cggacgcggc ggctccggct gagtctgcgc gcggctgccg ctgccgccga aaaggtctga
     1981 aaaaatgtct cgaaagctgg ttccgccggt accgccgccg ccgcccgagc ttcccgagct
     2041 ggcatcccac gtgaaaccgg aaaaatcgaa tccgcccgcg ccgcttcccg ctccggcgcc
     2101 cgcgccaaac ggcgaatccg ggtcgagatt gtcgttgtaa taaccgaaac ggtcgtaaac
     2161 cttgcgcttt ttctcgtcgg aaagaacgtc gtaagcttcc tgaacttctt taaatttttc
     2221 ctcggaggct ttatcattcg gattcacgtc cggatgatgc ttgcgcgcca gacggcgata
     2281 cgctttttta atctcgtccg ctttggcgtc tttttttata ccgagtatgc cgtaataatc
     2341 tttgtttgcc atttattaaa atttgccagc cgcaaatttt cggtctgccg aagaccaatt
     2401 ctcatttatc aaaaccaaag gcgaatttta tcgcctcaaa aacttcctgc attctttcat
     2461 cggaaagatg cgtgataact ttgccgagct tttcttttgg acggtttgaa tattatccaa
     2521 attaaccgcg cgaatttcgc tcattccatc gctttcgtcc agccaaactt ccgacggaag
     2581 gtttctgata gttgtcgtta ccggagcaat tgttatccag gttaaatccg tgatggcagc
     2641 gctccgcgtc agaatcaaag caggacgacg tttgtcagga gatttaaacg tgtgacaaca
     2701 aacatctccc tgtctcatac ctgtttccag aattcttcca tctgctcgtc ttcaacctca
     2761 aattcatcgg attgcaccgg aaatttttcg taggcttcct gatacatttt gccgatctct
     2821 tcttcggaaa gtttccgttt gcgcaaatcg tcgtgcaaag ttttccgcaa agactcatta
     2881 atatattgca cgcgattttt attgaaatct ttcgctacag cgtcaatttc agcaaccagt
     2941 ttttcgtcca attcaataaa aacgctcata gaattttcca gtaaaatcgg tgaacagcga
     3001 acagattttc tgaacactgt tcaccgacga ttcaactcga cgagatttta ctttttctct
     3061 tcgtcgacat caacatattc ggcgtcgatg acgttatcgt cgctcgtcgt cgaagatgtt
     3121 tcacccgcgc ccgcgcttgc cgaagaggct tgttcgccgc cggtttcagc gccgccggtc
     3181 tgagccgagc cagcctggct gtaaagaact tccgcgagtt tgtgcgaagc ggtttgcaga
     3241 cggttgaaag cgttgttcat cgcgtcggcg tcttcggcgc ccagagccgt tttcgattcg
     3301 gaaatagcgg cttcgatttc gcccgccgcg cctgcatcga gcttgtcctt gttctcgccg
     3361 aaagattttt cgaccgagta aaccaagccg tccagacggt tgcgcgcttc gatggtttcc
     3421 ttgcgtttgg tgtcttcgcc cgcgtgcgat tcggcgtctt tcatcatctt gtcgatttct
     3481 tccttcgaca agcccgaaga tgccgtaatc gtaatcttct gctcgcgtcc ggttccgacg
     3541 tctttcgccg aaacgttgac gatgccgttg gcgtcgatgt cgaaagtcac ttcgacctgc
     3601 ggaacgccgc gcggtgcggg cggaatgccg accagatgga attttccgag cgttttattg
     3661 tcggcagcca tcgggcgctc gccctgcaga acgtgaactt ccacgctggt ttgattgtcg
     3721 gacgcggtcg aaaagatttc cgatttgcgt gtcggaatcg tcgtgttgcg cccaatcatc
     3781 tgcgtcatca cgccgcccaa ggtttcgata ccgagcgaaa gcggcgtcac gtcgagaaga
     3841 agaatatccg ttttttcgcc gccgagaacc gccgcctgaa ctgccgcgcc gagcgcgacg
     3901 acttcgtccg ggttgacgga tttgttcggc tcttttccga aaaattcctt gaccatttcc
     3961 tgaactttcg ggatacgcgt cgagccgccg accagaacga tttcgtcaat ctgtcccttt
     4021 tcgactccgg cgtctttcaa agcctgctcg accggcggca tcaaacgctt caaaagcggt
     4081 tccgtcagcg cttcaaattt cgtccgcgtc attttcataa ccagatgctt cggtcccgat
     4141 tggtcagccg tgataaacgg caaattgatt tcggtttcca tcgcggacga tagctcgact
     4201 ttggcttttt ccgccgattc tttcaggcgc tgcaaagcca tcttgtcagc cgtcaaatcg
     4261 atgccctggt cttttttaaa ttcgtcgata atccatttaa tcaaggcttc gtcaacgtcg
     4321 tcgccgccta ggtgcgtgtc gccgttggtc gattttactt cgaccacgcc ttcgccgact
     4381 tcgagaacgg aaatatcgaa cgttccgccg ccgaagtcga aaaccgcgat ggtttcgtct
     4441 ttctttttat ccaaaccgta agccagagcg gcggcggtcg gttcgttgac gatgcgcatc
     4501 acttccagac cggcgatttt gccggcgtct ttcgtcgcct gacgctgcgc gtcgttaaaa
     4561 tacgccggaa cggtgataac cgctttatcg actttcgcgc cgagataatc ttcggcggct
     4621 tttttcaatt tctgcaaaac catcgccgaa acttccggcg ggctgtattg cttgccctgc
     4681 gcgtcgatgc gcacatcgcc gtttccggct ctttccactt taaagggcac ctgtttgatt
     4741 tcgccctgca cttcctcgta ttttcgtccc ataaaacgct taatcgaata gagcgtattt
     4801 tcgggattcg ttacggcttg acgctttgca acttgaccga caaggcgatt gccgtctttt
     4861 gtaaaagcaa ccaccgacgg cgttgtgcgc ccgccttctt cgttcgtgat gaccactggc
     4921 tcgccgcctt ccatcacggc gacaaccgag ttggtcgtcc ctaaatcgat accaataatt
     4981 ttactcattt cttaacttct ccgaacttat aatcgctatt gcttctcatc aagccgtttc
     5041 tcactataaa gacaacagca agttgaatat gtttatagaa taatatgtga gtgagttgtt
     5101 gtcaagttta tttgcataaa cttttacaca aaaagagcgg gattttactc gcgcttaacg
     5161 gatttttgtt gtgtaaacag gataaataaa accgctcgga agactttcag aaagaaaatg
     5221 acggctaacg gaatatacaa atacatgctt tgcacaaaaa taaaggaaaa tcgagaaaaa
     5281 ttccgatggc taattgtaaa ttctcaatta ttaattctca attacttaac aaaagatacc
     5341 gaacatcaat ttagaattta caatttacaa tttacaattt tccctttctt tatttttgtg
     5401 caaagccaca aatacaggga taacaggaca tacaaagcat taataagaca tccgtctcat
     5461 cgctgcctat cctgttatcc ccgttaaatt tcttatctgc aataaattgg taaaagaaat
     5521 ttattgcgca aatgccgcct gaaccggctt atcgctcggc aagccccatt gcacggcgta
     5581 atattgattg tccgagcttt tcagaatata ccagacgccc gcgcggaaga ttgcgaaatc
     5641 cgtcctttta tcgccgtcgt aatctgccgg aacgggaacg tcgccgctcg cgccccagtg
     5701 ctgaacccgc atcgagtttg tgctgctctg ccaaacgtac cagtcgccgt tgcgaaaaac
     5761 ggcgtaatcg gtttttttgt cgccgtcata atcagccgga acgggcttat cggttgaaat
     5821 tccgaactgc gtcgaggcga gcggcaattc gttttgatat ttgacatacc acgagccgcc
     5881 gcggaaaacg gcgatgtcgc ttcgccggtt gctgtcgtaa tccgaaggca gcggcacatc
     5941 gtttgcggag ccgaactgaa cgcccttgaa agcgccgtcc gagctttgca ggctgtacca
     6001 gacgccttcg cgaaaaacgc tcaaatccgc gcgcccgtcg ttgtcgaaat cggacggaac
     6061 gggtttgtcg ctcgcaatgc cgaaccggct ggcgaaataa tttccgtcag cgctcttttg
     6121 gacgtaccac gttccgtccc gataaacgcc gaagtcggtt tttccgtcgc cgtcgtaatc
     6181 ggcgggcacg agcttatcgg tcgatatgcc ccactgcgcc gccgtaaacg aaccgtctgt
     6241 actcttcaat acataccagc ttccggtttc gggacggaaa accgcgacat cggctttttt
     6301 gtcgccgtca aaatcgaaag ggacggcgcg cagttgcgcg accggttcga gtttgaagac
     6361 aaccaaattt atggattgat cgggaattac ggaatcgccc gccacgtaga cattgccctg
     6421 cgcgtcgagc aaaagattcg tctgcgaatt
//
LOCUS       DASWNE010000676         2103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_554092, whole genome shotgun sequence.
ACCESSION   DASWNE010000676 DASWNE010000000
VERSION     DASWNE010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554092"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            310..1020
                     /locus_tag="VGB68_13225"
     CDS             310..1020
                     /locus_tag="VGB68_13225"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEY0050249.1"
                     /translation="MQIETATASWHFGNRGRVAVFIDGNNLFHAARFHNIDIDYNKLL
                     RVLLGDGRLLRAFFYTGVDAGAERQQGFLLWMRRNGFRVVQKELKTFYDGTRKANLDV
                     EIAVDMLSLAEKYDTAVLVSGDEDFVYAVNAVAYKGCRVEVAGFRSNTAPRLIDVADF
                     FIDLGDIAELVRKDITSERYEIPSFVPPEELPPSVINQNHANDYSRITREIGIEPPNF
                     AETDDDSLADFVRVTDDR"
     gene            1017..1394
                     /locus_tag="VGB68_13230"
     CDS             1017..1394
                     /locus_tag="VGB68_13230"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HEY0050250.1"
                     /translation="MKLFEPTEASDLIPVVRPILQKIQARHADIARFRESANAAAEAA
                     QFGGGGMRGGSRYVKLLYELGKLTLEIDNLGVQLKDYARGLIDFPSWREGRVVLLCWQ
                     LGEGDEIEWWHEVEAGFGGRQPL"
     gene            1629..>2103
                     /locus_tag="VGB68_13235"
     CDS             1629..>2103
                     /locus_tag="VGB68_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050251.1"
                     /translation="MASSLSVCPSCGTMMNDSVREELALKISPIVKSAKPAIQKEIPK
                     PPIAPPVQQTPLQPPVQAASTQAPIQPERRGETIEQTSIPQAPAQQAPAQPPVLPKPV
                     QSAAPKPQTSEIASKATSPTLIDFRHKNAVVPEWRLHLQNVVRKRQDRTGDTQTDA"
BASE COUNT          483 a          542 c          528 g          550 t
ORIGIN      
        1 gtcctttaac caaaggtctt cgtgaatttg ttcgggcttt ttttccatcg ctttttgatt
       61 atacgataaa tttgaaatct atcggtcaat tttcgcttga ccgattcgct cgcttaacgg
      121 cagactataa agagttatat aaaatcgctt ttattgtttt ttgacagccc ggacgcgaaa
      181 gagaaagcta ttaaacaagt ccatattttt cggctattta aaaagttttt atcaatcttt
      241 taaacaaact tcggctctct tttcgaaaac tccggcggct gtctattttt tcaaagaggg
      301 ttatcaaaaa tgcagattga aactgctacg gcttcctggc atttcgggaa tcggggtcgc
      361 gttgccgtct ttatcgacgg aaataattta tttcacgcgg cgcgttttca taatattgat
      421 atcgattaca acaagctttt acgggttttg ctcggcgacg ggcgacttct ccgcgctttt
      481 ttctatacgg gcgtcgacgc cggcgcggaa cgccagcagg gatttctttt atggatgcgg
      541 cgcaacggct ttcgagtcgt ccagaaagag ctgaaaactt tttacgacgg cacgcgcaag
      601 gcgaatctgg acgtggaaat cgccgtcgat atgctctcgc tggcggaaaa atacgatacc
      661 gccgtcctgg tttcgggcga cgaggatttt gtttacgccg tcaacgccgt cgcctataag
      721 ggctgccgcg tcgaggtcgc gggctttcgc tcgaacaccg cgccgcgcct gatcgacgtg
      781 gcggattttt ttatagattt gggcgacatt gccgagctgg tgcgaaaaga tattaccagc
      841 gagcgctacg aaattccttc attcgtgccg ccggaagaat tgccgccgtc cgtcatcaat
      901 cagaatcacg cgaacgatta ctcgcgcata acgcgcgaaa tcggcatcga gccgccgaat
      961 tttgcggaga cggacgacga cagtctggcg gattttgttc gcgtcaccga cgatagatga
     1021 aactttttga accgaccgaa gccagcgacc tgattcccgt cgtgcgcccg attttgcaga
     1081 aaatccaggc gcggcacgcg gatatcgcgc gtttccgcga atcggcaaat gcggcggcag
     1141 aggctgctca attcggcggc ggcggaatgc gcggcggctc gcgttacgtg aaactgcttt
     1201 acgagctggg caaattaact ctcgaaatcg acaatctcgg cgtccagctg aaagattacg
     1261 cgcgcgggct tatcgatttt ccctcgtggc gcgaaggtcg cgtcgttttg ctctgctggc
     1321 agctcggcga aggcgacgaa atcgaatggt ggcacgaggt cgaagccggt ttcggcgggc
     1381 gtcagccgct gtaatttccg tttttcttat tcataatccg gctttttttc cagtttttca
     1441 catttttttc gcgtttcaag ccaaaattcc ctgtattttc ataaatttac tattgactac
     1501 ttaaagtagt tcctgatagt ctgttcacag tttggtcata tcgtttggtt ttcaaccaaa
     1561 actcgcgttt cactttttcc aattttcaat attttaaaac tatgaattgt cccggttgca
     1621 ataaaatttt ggcttctagt ttgtcggttt gcccgagctg cggaacgatg atgaacgatt
     1681 ccgtgcgtga agaactggct ttgaaaattt caccgattgt aaaatccgca aaacccgcga
     1741 tacaaaaaga aattccaaag ccgcccattg cgccgcccgt tcagcagact ccgcttcagc
     1801 cgccggtgca ggctgcctct acgcaggctc cgattcaacc tgagcggcgc ggcgaaacga
     1861 ttgagcagac ttcaattccg caagcgcccg ctcagcaagc gcccgctcag ccgccggtgc
     1921 tgccgaaacc cgtgcagagt gccgcgccga aaccgcaaac ttcggaaatc gccagcaaag
     1981 cgaccagccc gacgctgatt gattttcgtc ataaaaacgc ggtcgttccc gaatggcgtc
     2041 tgcatttgca aaacgtggtg cgcaagcgtc aggatcgcac cggcgacacg caaaccgacg
     2101 cag
//
LOCUS       DASWNE010000677         2162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_271448, whole genome shotgun sequence.
ACCESSION   DASWNE010000677 DASWNE010000000
VERSION     DASWNE010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271448"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..1471
                     /locus_tag="VGB68_13240"
     CDS             215..1471
                     /locus_tag="VGB68_13240"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY0050252.1"
                     /translation="MNSKILSLVLLFLFSSVVSSAASAGDGEYTGQFEAALVPNTENF
                     ERVIFKYASAATVNSESRFDQKASLARGRLYDPQTATASVAALLVDEEDDEKKPLIFV
                     DLDGNGSFSNSEKFAFERTEKDNPYLWNITVNLPVKDNFFTACPLFLQYFKSVRIEKM
                     GEGDRLLTQSTEVLARGRVEVKGKKILAQYAYSFEDKKITPQKGWLGIDGNEDGAVDM
                     DELSPEAAKADDETIVFRVGQTYLSTKKVDLAKNQIVMREHAAKDYQRIELSVGKEFP
                     EFSFVDLTGKKRRFSEFRGKYILLDVWGFWCPPCRREMPFLREAAKRFQTRNLEIVGL
                     NTDVEMPLDVIKRNLEANDLKWTQARLESVFDLINKQLRIESFPTTFLIAPDGKILSM
                     SRHLRGEPDLRGKDLLETLDEILPKQ"
     gene            complement(1664..2053)
                     /locus_tag="VGB68_13245"
     CDS             complement(1664..2053)
                     /locus_tag="VGB68_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050253.1"
                     /translation="MPTLNRARFTSTTADDNKDKDTGVYVYVYTSNGATRLAQIENAD
                     NSDDDATEYNDNSRHSIDLSVLSASEKSDCVNFTFKVGSKANGNDKWKIRDASVELFF
                     TDGTNLTRSTGAFELNSKGSKYTETSF"
BASE COUNT          607 a          494 c          527 g          534 t
ORIGIN      
        1 cgaccggcgg gcttataaag ataagcgcca ctcagctcac aaacccgatg cggttttaat
       61 ttatcttatc aaataaaaaa aattattgat gactgccggc agattgtcga aaatccgtcg
      121 aagttttgcg cggtaaagcc cggctttgcg gatagcgcgg aaattgcgaa gcgaagccga
      181 acgccggtca tttctacaag gaaacaccaa aatcatgaat tctaaaatat taagtctcgt
      241 cctgcttttt ctgttttcga gcgttgtttc atccgccgct tcggcgggcg acggcgaata
      301 caccgggcaa ttcgaagccg cgctcgtacc gaatacggaa aattttgaaa gagttatttt
      361 taagtatgct tcggctgcaa ccgtaaacag cgaaagccgt tttgaccaga aggcgagcct
      421 ggcgcgcggg cggctttacg acccgcaaac cgcgaccgct tccgtggcgg cgctgctggt
      481 cgacgaagag gacgacgaaa aaaagccgtt gatttttgtc gatttagacg gcaacggcag
      541 tttttccaac agcgagaaat tcgcgttcga acggacggaa aaagacaatc cttacctgtg
      601 gaacatcacg gtcaatctgc ccgtcaaaga taattttttc accgcctgcc cgctttttct
      661 gcaatatttc aaatcggtgc gcatcgaaaa aatgggcgag ggcgaccgcc tgctgacgca
      721 atcgaccgaa gttctggcgc gcggcagagt cgaggtcaaa ggcaaaaaga ttctggcgca
      781 atacgcctac agctttgaag ataaaaagat aacgccgcag aaaggctggc tcggcatcga
      841 cggcaacgaa gacggcgcgg tcgatatgga cgagctttcg ccggaagccg ccaaagccga
      901 cgacgagacg attgttttcc gcgtcgggca gacgtatctc tcgacgaaaa aagtcgattt
      961 ggcgaaaaat caaatcgtga tgcgcgagca cgcggcaaaa gattaccagc gcatcgagct
     1021 gagcgtcggc aaagagtttc ccgaattttc gtttgtggat ttaaccggaa agaagcgccg
     1081 tttttccgag tttcgcggca aatatattct gctcgacgtg tggggtttct ggtgcccgcc
     1141 gtgtcgccgc gagatgcctt ttctgcgcga agccgccaag cgttttcaaa cgagaaatct
     1201 ggaaatcgtc ggtctgaata ccgatgtcga gatgccgctc gacgtcatca aaagaaatct
     1261 ggaagcgaac gatttaaaat ggacgcaggc gcggctcgaa agcgttttcg atttaatcaa
     1321 caagcaattg cgcatcgaat cgtttccgac gacgttttta atcgcgcccg acggaaaaat
     1381 tctttcgatg agccgccacc tgcgcggcga gccggatttg cgcggcaagg atttgctcga
     1441 aacgctcgac gaaattctgc cgaaacagta atttaaaggc aaaaagtaaa aagcgaaaag
     1501 gcaaaatgag aacaatttcg gcgttgcctc atagaccttg taaaaggcaa tctgtaaggc
     1561 aagttaagca gataaaaggc aaatattgaa tcaggttgaa ttttatttag ttatttcaac
     1621 ctgattcaat gcctatatat aaaccagtaa caacacaacg tctttagaac gaagtttcgg
     1681 tatattttga acctttgctg ttaagttcaa atgcgcctgt gcttctagtt agattcgtcc
     1741 cgtccgtgaa aaaaagttcc acacttgcgt cccgaatctt ccacttatca ttgccatttg
     1801 cctttgagcc aactttgaaa gtaaagttta cacagtcgct tttctcagaa gcacttagca
     1861 cactcaagtc gattgaatgt cttgagttgt cattatactc agtcgcatca tcatcggaat
     1921 tgtctgcatt ctcaatttga gcgagtctgg tagcaccatt ggacgtataa acgtagacgt
     1981 atacgccagt atccttatcc ttgttatcgt ctgctgtagt tgaagtgaaa cgagcgcgat
     2041 tcaaagtagg catagctatt tatcctccta atgttgtgta agaatcttaa agcagcagca
     2101 agtgtatata aggattgtga attggtcaag agtaaaattt tacagtggat taagcctgaa
     2161 at
//
LOCUS       DASWNE010000678         2333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_481723, whole genome shotgun sequence.
ACCESSION   DASWNE010000678 DASWNE010000000
VERSION     DASWNE010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481723"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..983)
                     /locus_tag="VGB68_13250"
     CDS             complement(186..983)
                     /locus_tag="VGB68_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050254.1"
                     /translation="MKLTNLTKLLMLACICFLSTATFAQDTQKTLQKPYQKWSQAEAM
                     KVISNQPWADQYQSERGLAAVSQSNQAREQSDTNLGGRNRGSGSRVLAPPPITIRLHS
                     ALPVRQAMVRLQQLQIGYDKMNEEDRNKFDDSKKVFLNCPACQNYYVVTITKWKDSSP
                     GAVDDGIFQTMKFDDLKGKVWLVNEKNERRELVQFTPPKGAGESAWFFFKRTDDKGNL
                     LLTPENKELRFVFDNKLLDDTNAYSALIPRAFEFKVAKMMVNNKVEF"
     gene            complement(1044..>2333)
                     /gene="rimO"
                     /locus_tag="VGB68_13255"
     CDS             complement(1044..>2333)
                     /gene="rimO"
                     /locus_tag="VGB68_13255"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEY0050255.1"
                     /translation="MMGQLKEAGYEITSQEADAEIVVVNTCGFIESAKQESVEAILEA
                     TSLKAEGKAKRVIVAGCLVERYRDDLIKELPEVDAFIGTNQINDILKAADADINFREL
                     PLLPIGNKTATYLYDFDTPRYRATDSYTAFIKIAEGCDRPCAFCSIPSMRGSFRSRRF
                     GSILKEAEDLARAGVREVVLIAQDSSRFGEDLGEVDALAKLVRALGEIEDLEWIRVMY
                     AYPTHISDAFLAAIAETPKAVKYLDMPLQHASRNVLKLMKRGGNRESLERLIARVREK
                     VPGITIRTTFITGFPGETEADFEELMAFVRNCEFDNVGVFTYSDEEGTPAFGLPDKVD
                     AKTAKRRRAALMKEQAKISKRKNKSKIGKTFKILFEGVSQESDLLFQGRLEGQAQEID
                     GYVLINDMPEDFEPAIGKFYSVKITDALNYDLIGEII"
BASE COUNT          565 a          600 c          523 g          645 t
ORIGIN      
        1 cttctttata attatcctgc tctatgcagc gggcagttta tgctccgccc gcgccgccga
       61 gtcagtaatt tttaactatc cgcatcgcag aaattcgcaa tcttagggca aatactctaa
      121 gattgcgaat tcgaaaacga cgtcgaaaat tccaaaacgg attctccccg aattgtcgcc
      181 taaaactaaa attcgacttt gttattgacc atcattttcg caactttgaa ttcaaaagcc
      241 cgcgggataa gcgcgctgta agcattcgta tcatccagca acttattgtc gaaaacgaat
      301 ctgagttctt tgttttcagg cgttaataaa agatttcctt tatcatcggt tcttttaaag
      361 aaaaaccaag ccgattcgcc cgcgcctttc ggcggcgtga actgcaccag ctcgcgtctc
      421 tcattttttt cgttgaccag ccagactttg ccttttaaat cgtcgaactt catcgtctga
      481 aatattccgt catcgaccgc accgggcgag gaatctttcc acttcgtaat ggttacgaca
      541 taataattct ggcaagccgg acaattcaaa aagacttttt ttgaatcgtc aaatttattc
      601 cggtcttctt cgttcatctt gtcataaccg atttgaagct gttgcaatct gaccatcgcc
      661 tgtcgcacgg gcaaagccga atgcagcctg atggttatcg gcggcggcgc gaggacacgc
      721 gaaccgctgc cgcgatttct tccgccgaga ttcgtgtcgg attgctcgcg cgcctgattc
      781 gactgcgaaa cggcagctaa acctctctcc gattgatact ggtcagccca aggctgattt
      841 gagataactt tcatcgcttc cgcctggctc catttttgat aaggtttctg caaagttttt
      901 tgagtatcct gcgcgaatgt cgccgtcgac aaaaaacaga tgcaagccag cattaataat
      961 ttagttaaat tagttaattt catatttgat tgtacttcct gagtaataga attttgtgga
     1021 aaaacctcgt tggtaaatgt atattaaata atctcgccaa tcaagtcata attgagcgca
     1081 tcggttattt ttactgaata aaatttgccg attgccggct cgaaatcttc gggcatatcg
     1141 ttgatcaaaa cgtagccgtc gatttcctga gcttgtcctt cgagtcgtcc ctgaaaaagc
     1201 aaatccgatt cctgcgaaac gccttcaaac aaaattttga aagttttgcc gattttcgac
     1261 ttgtttttgc gtttggaaat tttcgcctgt tctttcatca gggcggcgcg gcgacgcttc
     1321 gcggttttcg cgtcaacctt gtccggcaaa ccgaaggcgg gcgttccctc ttcgtcggaa
     1381 taagtgaaaa cgccgacgtt atcgaattcg cagttgcgta caaaagccat caattcttca
     1441 aaatccgctt cggtctcgcc cggaaagccg gtgataaaag tcgtgcgaat cgtgatgccc
     1501 ggaacttttt cgcggactct ggcaatcaat ctttccagac tttcgcgatt tccgccgcgc
     1561 ttcattaatt tcaggacgtt gcgcgaagcg tgctgcaaag gcatatccag atatttgacg
     1621 gctttcggcg tttccgcaat cgccgcgaga aaagcgtcgg aaatatgcgt cgggtaagca
     1681 tacatcacgc gaatccattc caaatcttca atctcgccga gcgctcgaac caatttcgcc
     1741 aacgcgtcga cctcgcctaa atcttcgccg aaacgcgaag aatcctgcgc gatcaaaacc
     1801 acttccctga cgcctgcccg cgccaaatct tcggcttctt ttaaaatcga gccgaaacgg
     1861 cgcgaacgaa acgagccgcg catcgaagga atcgaacaga aagcgcacgg gcggtcgcag
     1921 ccttcggcga ttttgatgaa agccgtgtaa gaatcggtgg cgcgatagcg cggcgtgtcg
     1981 aaatcgtaaa gataagtcgc cgttttattg ccgatcggca gcagcggcag ctcgcggaaa
     2041 ttgatgtccg cgtcggcggc tttcagaata tcgtttatct ggttggtgcc gataaatgca
     2101 tcgacttcgg gcaattcctt tatcaaatcg tcgcgatagc gctcgaccag acaaccggcg
     2161 acgataactc tttttgcctt tccttcggct ttcagtgatg tcgcttcgag aatcgcttcg
     2221 accgactcct gcttggcgga ttcgataaag ccgcaggtat tgacgacgac gatttccgcg
     2281 tccgcttcct gtgatgtaat ttcgtagccg gcttctttta attgtcccat cat
//
LOCUS       DASWNE010000679         3275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_271497, whole genome shotgun sequence.
ACCESSION   DASWNE010000679 DASWNE010000000
VERSION     DASWNE010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271497"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1418)
                     /locus_tag="VGB68_13260"
     CDS             complement(<1..1418)
                     /locus_tag="VGB68_13260"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEY0050256.1"
                     /translation="MKTILKGKRQTAKGKNKFANFARRFFAFYFLLFTFAFNCLHLRE
                     ASAQVRPVYDYGATGLAQLLKRLNTTASAMHIGAHPDDEDSALLAFLARGENARTAYL
                     SLTRGDGGQNIIGSELFESLGVIRTEELLQARRLDGAEQYFTSAFDYGFSKTLAEARE
                     KWDEKIILCDAVRAIRTFRPLVVISRFSGTPADGHGQHQFAGYIAPIAVKAAADSGQC
                     KDAGAPWQVLKFYVSQGFRSQAQPSLRLNTGRYDYLLGRSYFEIAMEGRSQHKTQEQG
                     VLELKGERFSGLNLVESKAAKVESETSVFDGIDTSLENIPRLFGKDYNSVGEYTKDRL
                     KIIRESAELAAKEYNPNNFQKIVPALVQGRKAADELFSSYTFFSGTEERKKKFDERIA
                     NEVDLSFIAYKKLLQFTETLKLAAGIQIEALADREPVAPGENFTASVKVFYPENPNIK
                     VAGVKLYAPQRSFRIQSAEEPE"
     gene            complement(1451..1624)
                     /locus_tag="VGB68_13265"
     CDS             complement(1451..1624)
                     /locus_tag="VGB68_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050257.1"
                     /translation="MNPDFQRRFCEIFERLFVAGETEAVIELTGELLAPHGGFLFDGL
                     RLDAPADWRKPVE"
     gene            complement(1664..2197)
                     /locus_tag="VGB68_13270"
     CDS             complement(1664..2197)
                     /locus_tag="VGB68_13270"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY0050258.1"
                     /translation="MKKKTSLEELLEITKEILRTKYASAEFAFLSGSIVRGEATAFSD
                     LDIVVIYKELPNAFRECFYFRDFPVETFVHTPETLSYFYEFDAKTFVPSLASMVAEGI
                     EVPSKNDLSARLKHSANEFLSRPPKISVEEIRDFRGWLTDLIDDIREPRSKAELTASG
                     TTLYAALADFYLRADGG"
     gene            complement(2175..>3275)
                     /locus_tag="VGB68_13275"
     CDS             complement(2175..>3275)
                     /locus_tag="VGB68_13275"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050259.1"
                     /translation="GVCFIVILAGFPEGVSLADGLYLAGSLGKLTTVDTSFNLQEKYT
                     IWSGLIGGLFLMLGYFGCDQSQVQRFLTAKSIGEGRTSLLMSAFLKIPMQFFILLIGV
                     LVFVFYQFNAPPIVFNVNEVAKAGQNAQFQTIQQNYAEAHAERREAAIKFATESREFR
                     QNYIEADKKFNESRREAVKFVRETSDPKFNDVNYVFPTFVLENMPMGVIGLIIAAIFA
                     AAMSSISAELNALATATTIDFYRRLYRPEATDAQTVAVGRISTFIWGIFACIVAVFAT
                     NLGSLIEVVNKFGSFFYGSLLGVFVLAFVVKRAKARGAFFGLLFGIASVWIASNYTNI
                     ELLWFNVIGCLVTVLFGYLISLTVRNEEKNVA"
BASE COUNT          800 a          899 c          813 g          763 t
ORIGIN      
        1 ttttcaggct cttcggcgga ctgaatccgg aagctccgct gcggggcata aagcttgacg
       61 ccggcaacct taatattcgg gttttcggga taaaaaactt ttaccgacgc tgtaaaattt
      121 tcgcccggcg cgaccggttc ccggtcggct aaagcctcga tttgaattcc cgccgccagc
      181 ttcagcgttt cggtaaattg aagcagtttt ttataggcga tgaaagaaag gtcgacttca
      241 ttggcaattc tttcgtcaaa tttctttttc cgttcttcgg ttcccgaaaa aaacgtgtag
      301 ctggaaaaca gttcatcggc ggcttttctt ccctgcacca gagccggcac gattttttgg
      361 aaattattcg gattatattc tttcgccgcc agctcggctg attcccttat tatctttaaa
      421 cggtctttcg tatattcgcc gacggaatta tagtctttgc cgaacaatct cggaatgttc
      481 tccagcgaag tgtcgatgcc gtcaaaaacg ctcgtctcgc tttcgacttt cgccgctttg
      541 ctctcaacca gattcaagcc cgaaaaccgc tctcccttaa gttccagaac gccttgttcc
      601 tgcgttttgt gctggcttct gccttccatc gcgatttcga aatacgagcg tccgagcaga
      661 taatcgtatc tgcccgtgtt caagcgcaag ctcggctgcg cctgcgagcg aaagccctgc
      721 gaaacataga attttaaaac ctgccacggc gcgcccgcgt ctttgcattg accggaatcg
      781 gcggcggctt tgacggctat cggcgcgatg tagccggcaa attggtgctg cccgtgaccg
      841 tcagccggag ttccggagaa acgcgagatg acgaccagcg ggcgaaacgt ccggatagcg
      901 cgcaccgcgt cgcacaaaat tattttttcg tcccattttt cccgcgcttc ggctagagtt
      961 ttggaaaaac cgtaatcgaa agcgctcgta aaatactgct ccgcgccgtc cagccgccgc
     1021 gcctgcaaaa gctcttcggt gcgaatcacg cccagggact cgaacaattc cgagccgata
     1081 atattctgcc cgccgtcgcc gcgcgtcagg gataaataag ccgtccgcgc attttcgccg
     1141 cgcgccagaa aggcgagcag cgctgaatct tcgtcgtccg gatgcgcgcc gatgtgcatc
     1201 gcgctggcgg tcgtgtttaa tcttttaaga agctgcgcca gaccggtcgc gccgtaatcg
     1261 taaacgggtc ggacctgcgc cgaagcttcg cgcaaatgca ggcaattaaa ggcgaaagta
     1321 aaaagcagaa agtaaaaagc aaaaaaccgg cgagcaaaat tcgcgaattt atttttgcct
     1381 ttcgccgtct gccttttgcc ttttaagatt gtcttcattc gtgattccgt ttcttttttc
     1441 caactttaat ttattcgacc ggctttcgcc aatcggcggg cgcatcgagc cgcaagccgt
     1501 caaacaaaaa accgccgtgc ggcgcgagca attccccggt cagctcgatg accgcttcgg
     1561 tttcgcccgc gacgaataat cgttcgaaga tttcgcaaaa tcgccgctga aaatcaggat
     1621 tcaattttcg caaggctcgg ggaatcgatt tgcttttcgc cgatcagccg ccgtctgcgc
     1681 gcagataaaa atccgccaga gcggcgtaaa gcgtcgtccc ggaagcggtc aattccgcct
     1741 ttgagcgcgg ctcgcgtatg tcgtcaatca aatcggtcag ccagccgcga aaatcgcgaa
     1801 tttcctcgac ggaaattttc ggcgggcgac tcaaaaattc gttcgccgaa tgtttcagac
     1861 gcgcggacaa atcgtttttc gatggaactt cgatgccttc ggcaaccatc gaggcaagcg
     1921 agggaacaaa agtttttgcg tcgaattcgt aaaaataact cagagtttcc ggcgtgtgaa
     1981 cgaaagtctc gaccggaaaa tcccggaaat aaaaacactc ccggaaagcg ttcggcaatt
     2041 ctttgtagat aacgacgatg tccaaatcgg aaaacgccgt cgcttcgccg cgcacgattg
     2101 agccggacaa aaaagcgaat tcggcggacg cgtatttcgt ccgcaaaatt tctttcgtga
     2161 tttcgagcag ctcttcaagc gacgtttttt tcttcatttc ttaccgttaa gctaatcagg
     2221 tatccgaaca aaaccgtgac cagacagccg atgacgttaa accataacaa ttcgatattc
     2281 gtgtaattgc tcgcaatcca aacggacgcg atgccgaaaa gcagaccgaa aaacgcgccg
     2341 cgcgcctttg cgcgtttgac gacgaaggcg agcacgaaaa cgccgagcag cgagccgtaa
     2401 aaaaatgagc cgaatttgtt gacgacttca atcagagagc ctaaattcgt cgcgaaaacc
     2461 gccacgatgc aggcgaaaat tccccagata aacgtcgaga ttctgccgac ggcgaccgtc
     2521 tgcgcgtcgg tcgcttcggg gcgatacaag cgccggtaaa aatcaatcgt cgtcgccgtg
     2581 gcgagggcgt tcagctcagc cgaaatcgag ctcatcgccg ccgcgaaaat cgccgcgatg
     2641 atcaagccga taacgcccat cggcatattt tcgagaacaa aggtcgggaa aacgtaattg
     2701 acatcgttaa atttcgggtc gctcgtctcg cgcacgaatt taacggcttc ccggcggctc
     2761 tcattaaatt ttttgtcggc ttcgatatag ttctgccgaa actcgcggct ttcggtcgca
     2821 aattttatcg cggcttcgcg gcgttcggcg tgcgcctcgg cgtaattttg ctgaatcgtc
     2881 tgaaactgcg cgttttgccc ggctttggcg acttcattga cgttaaaaac aatcggcggc
     2941 gcgttgaact ggtaaaaaac gaaaaccagc acgccgatca gcaaaatgaa aaactgcatc
     3001 gggattttca gaaacgcgct catcaaaagc gaagttctgc cttcgccgat cgatttcgcc
     3061 gtcaaaaatc tctgcacctg gctctggtcg cagccgaaat agccgagcat cagaaaaagc
     3121 ccgccaatca aacccgacca aatcgtgtat ttttcctgta gattaaagct cgtatcgacc
     3181 gtagtcagtt ttccgagcga gccggcgaga taaagcccgt cggcgagcga gacgccttcg
     3241 ggaaagcccg ccaaaatcac gatgaaacaa acgcc
//
LOCUS       DASWNE010000680         3476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_234195, whole genome shotgun sequence.
ACCESSION   DASWNE010000680 DASWNE010000000
VERSION     DASWNE010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234195"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..2299
                     /locus_tag="VGB68_13280"
     CDS             185..2299
                     /locus_tag="VGB68_13280"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY0050260.1"
                     /translation="MQHLKIFFLIAIFMSLPMISTAQKLEYPQTKKVEQTDNYHGTKI
                     ADPYRWLEDDNSAETKAWVEAQNKVTFAYLNRIPEREQIKKRLTELWNYEKYSAPFKR
                     GSKYFYYKNDGLQNQSVLYVADSVTDKGRVLLDPNKLSADGTIALSGLAISDDGKLMA
                     YGLAGAGSDWQEWRFMDVETGKDLPDVLKHIKFSGASWTLDGKGVYYSRYPETDAKSK
                     LSAENFHQKLYFHRLGTPQTEDALVYERTDDKEMFVGGFVSEDGNWLIINVGKGTSPM
                     NMVFVKNLSTADAPVIPVIDRLEADYSFVGNDGSTFYFRTDKDAPRGRMVSIDVLAKD
                     KKWNEVIAQSAETLEGVDFINNQFVGSFLKDAYTQFRVYDLGGKFVRNVELPGIGSAG
                     GFSGKRFDTETFYTYSSFNTPPTIYSYDMKSGKSEIFRKSDVKFNPADYEVKQIKYKS
                     KDGTEIPMFIVHKKGLKLDGSNPTLLYAYGGFNVSMTPGFSVSRLVWLERGGVFAMPN
                     LRGGGEYGEQWHAAGTKLQKQNVFDDFIAAAEWLIASKYTQPAKLAISGGSNGGLLVG
                     ATLNQRPDLFGAALPAVGVMDMLRFHKFTIGRAWTSDYGSADNAEEFKAIYAYSPIHN
                     IKKGAKYPAVLVTTADHDDRVVPAHSFKYAATLQEMQGGEAPVLIRIETKAGHGAGKP
                     TAKIIEEQADIYGFLMKNLGMK"
     gene            complement(2518..3111)
                     /locus_tag="VGB68_13285"
     CDS             complement(2518..3111)
                     /locus_tag="VGB68_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050261.1"
                     /translation="MNFKNILFRLVAVLTAFILGVAFFNAAQYLQTFFRTPESAALPS
                     AVKKENLFVPPRSAPTAVFDAPQTVAESEEDIGCEYDGYGDYLIEGDLPRGFKGFDRI
                     NIVTRNFKAKSKDCPIGTPIPPQGRLQTTKEYKFTRISIGNKQITFETETIKRISYKF
                     AGKFEPGKAEGLLIKIRDGKLAAESQVRFLLDEKGGC"
     gene            3259..>3476
                     /locus_tag="VGB68_13290"
     CDS             3259..>3476
                     /locus_tag="VGB68_13290"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEY0050262.1"
                     /translation="MKIEMLGEAAKDAKTRAERIADSTGNQIGAVRSARMGVMQITAA
                     DSTEVSDAGISDSTTIDKDVTAVVNISFA"
BASE COUNT          899 a          881 c          871 g          825 t
ORIGIN      
        1 atcgttcaag ggaacctttg aatcgtttca gggaaccttt gaatcgttca aggattccct
       61 gaaacgattc aaagcggcgc cggaatagtt tacgggctta aaacgtgttc ggcagtggct
      121 ttttctaatc ttcactccgt agtaaaatca ttggaaattc caaaacattc cggagaggta
      181 accgatgcag catctgaaaa tcttcttttt gattgctatt tttatgtctt taccaatgat
      241 ttctacggct caaaagcttg aatatccgca aacgaaaaag gtcgagcaga ccgataatta
      301 tcacggcacg aaaatcgccg acccgtatcg ctggctggaa gacgataatt ccgccgaaac
      361 aaaagcctgg gtcgaagcgc aaaacaaagt cacttttgct tatttaaacc ggattcccga
      421 acgcgaacag ataaaaaaac gtctgaccga gctttggaat tacgaaaaat attccgcgcc
      481 ctttaaacgc ggcagcaaat atttttatta caaaaacgac ggcttgcaga atcagagcgt
      541 cctatatgtc gccgattccg ttacggataa aggtcgcgtt ctgctcgacc cgaacaagct
      601 ttcggcggac ggaacaatcg ctctgtcggg cttggcgatt tccgacgacg ggaaactgat
      661 ggcttacggc ttggcgggcg cgggttcgga ctggcaggaa tggcgtttta tggatgtcga
      721 aaccggaaag gatttgcccg acgttttgaa acacatcaag ttttccggcg cttcgtggac
      781 gctcgacggc aagggcgtct attattcgcg ctacccggag acggacgcca aatcgaaact
      841 gtccgccgaa aactttcacc agaagcttta ttttcaccgt ctcggcacgc cgcagaccga
      901 agacgcgctg gtttatgagc gcacggacga caaggaaatg tttgtcggcg gctttgtctc
      961 cgaagacggc aactggctga ttatcaacgt cggcaaaggc acttcgccga tgaatatggt
     1021 tttcgtcaaa aacctttcga cggctgacgc gccggtcatt cccgtcattg acaggctcga
     1081 agccgattac agctttgtcg gcaacgacgg ctcgacgttt tattttcgca cggacaaaga
     1141 cgcgccgcgc gggcggatgg tttcaatcga cgttctggcg aaagataaaa aatggaacga
     1201 agtcatcgcg caatcggcgg agacgctcga aggcgtcgat tttatcaaca atcaattcgt
     1261 cggaagcttc ctgaaagacg cttacacaca gtttcgcgtc tacgacctcg gcggcaaatt
     1321 cgtccgcaac gtcgagctgc ccggaatcgg ctcggcgggc ggtttcagcg gcaagcgctt
     1381 tgacacggaa actttctaca cttattcgag ctttaacacg ccgccgacga tttacagcta
     1441 cgatatgaag tcgggcaaat ccgaaatctt tcgcaaatcc gacgtgaaat tcaatccggc
     1501 ggattacgaa gtcaaacaaa tcaaatataa aagcaaggac ggaacggaaa ttccgatgtt
     1561 catcgtccac aaaaaaggct tgaagctgga cggctcgaat ccgactttgc tttatgctta
     1621 cggcggcttt aacgtttcga tgacgcccgg cttttcggtt tcgcggctgg tctggctgga
     1681 acggggcgga gttttcgcga tgcctaacct gcgcggcggc ggcgaatacg gcgagcagtg
     1741 gcacgccgcc ggcacgaagc tgcaaaagca gaacgttttt gacgatttca tcgccgcggc
     1801 tgaatggctg attgccagta aatacacgca gcccgccaag ctcgccattt ccggcggctc
     1861 aaacggcggc ttgctcgtcg gcgcgacgct caatcagcga ccggatttat tcggcgcggc
     1921 gcttccggcg gtcggcgtga tggatatgct tcgttttcac aaatttacca tcgggcgcgc
     1981 gtggacttcc gattacggct cggctgacaa cgcggaagaa ttcaaagcga tttacgccta
     2041 ttcgccgatt cacaacatca aaaaaggcgc gaaatatccg gcggttctcg tgacgaccgc
     2101 cgaccacgac gaccgggtcg ttcccgcgca cagctttaaa tacgcggcta ccttgcagga
     2161 aatgcagggc ggcgaagcgc ccgtactgat tcgcatcgaa accaaagccg gacacggcgc
     2221 gggcaagccg acggcgaaaa taatcgaaga acaagccgac atttacggct ttttgatgaa
     2281 gaatctggga atgaaataaa gcctccggat aaaattaaaa gggcagacgc aaatctctgc
     2341 ccttttgctt tgcacaaaaa taaaggaaat cgagaaaaat tccgatggct aattgtaaat
     2401 tctcaattat taattctaaa ttgcttcaca aaagataccg aacatcaatt tagaatttac
     2461 aatttacaat tttccctttc tttatttttg tccaaagccc tgcccttttt cattttatta
     2521 acatccgccc ttctcatcga gcaaaaaacg aacctgactt tctgccgcga gttttccgtc
     2581 ccgtattttg attaaaagac cttccgcctt tccgggctcg aattttccgg cgaatttgta
     2641 gcttattctt tttatggttt cggtctcgaa agttatttgt ttattgccga tgcttattcg
     2701 agtaaattta tattctttcg tcgtttgcag gcgaccttgc ggcggaatcg gagttcctat
     2761 cgggcaatct ttcgatttag ccttaaaatt tcgggtgacg atgtttatcc ggtcgaaacc
     2821 tttaaatcct ctcggcaaat cgccttcaat caaataatcg ccgtaaccgt catactcgca
     2881 gccgatatct tcttcggatt cggcaactgt ctgcggcgcg tcgaaaacgg cggtcggcgc
     2941 ggagcgcggc ggcacaaaca aattttcctt tttgacggcg gacggaagcg ccgcagactc
     3001 cggcgtccgg aaaaaagttt gcagatattg cgcggcgtta aaaaaagcga cgccgagaat
     3061 aaacgccgtc aggacggcga cgagccgaaa caaaatgttt ttgaagttca taatccgctc
     3121 ccgcttattt ttattcgacg gcgaaactga tattgacgac cgccgtcata tctttatcta
     3181 tcgaagacgt gtcgctgatg ccggaatcgg aaacttcggt cgaatccgcc gcagtattac
     3241 tatactaagc tcggcgattt gaaaatcgaa atgctcggcg aggcggcgaa agacgcgaaa
     3301 acgcgcgccg aacgcatcgc cgacagcacc ggcaaccaaa tcggcgcggt gcgctcggca
     3361 agaatgggcg tgatgcagat tacggcggcg gattcgaccg aagtatcgga cgcgggcatc
     3421 agcgattcga cgacgatcga caaagacgtt acagcagtcg tcaatatcag ttttgc
//
LOCUS       DASWNE010000681         1787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_481808, whole genome shotgun sequence.
ACCESSION   DASWNE010000681 DASWNE010000000
VERSION     DASWNE010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481808"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VGB68_13295"
     CDS             complement(<1..310)
                     /locus_tag="VGB68_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050263.1"
                     /translation="MSSTASDDFKAYRISFERRPDYLYAYVGGERDSLAISLEFWREI
                     AAECQRAQAKKVLIEEDIEESVSMLEMYQIAAEIPQMGFADVLIAFVDRFLEQQSLNE
                     F"
     gene            1111..>1787
                     /locus_tag="VGB68_13300"
     CDS             1111..>1787
                     /locus_tag="VGB68_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050264.1"
                     /translation="MSIPGSNFPLRNRRIIAVLSALLLLAVVGSTLASAAEISLFGSF
                     KQLIGFNAESSVAASPQPVVTTQPLTIGTCDTAGPIEVESTGGTTAPTAYATLAAAFT
                     AINAGTHTGSINIEVCGNTTETASATLTASGLASTSYTDVTIRPVGGARIIEGSVVGA
                     IIRLNGADNVTIDGRQGGAGTARDLTVRNNSTAAATAAVWLSSVAAGNGAASNTVRNL
                     ELAGGTDT"
BASE COUNT          471 a          448 c          430 g          438 t
ORIGIN      
        1 cgaattcgtt taaactctgc tgttccagaa aacggtcgac gaaagctatt aaaacgtcgg
       61 cgaatcccat ctgcggaatt tcggcggcga tttgatacat ctccagcatc gagacggatt
      121 cttctatatc ttcttcaatc aaaacttttt tggcttgcgc cctttggcat tcggcggcga
      181 tttcgcgcca gaattccagc gaaatcgcca ggctgtcgcg ctcgccgccg acgtatgcgt
      241 aaagataatc cgggcgtcgt tcaaagctga ttcggtatgc tttgaagtca tctgaagcgg
      301 tgctgctcat aaatttaatc ttagtaattt tcaaacggaa aatgcaaagc ccgccctata
      361 aacaagaaag ctcgaaactg aaaaaccccg cgcggcggca aacggtttcg gacgcgaaca
      421 agtcttaatt aatatttatt atttgtgtat ttttacatat gatgttaaat gacttgacat
      481 taataacaat ttgcctataa ttcaacgagt tctaataatt ccaaacataa agctggtatt
      541 ctgcgaacac aaaagatcgc ccaggggcac aagtcgccca tttaattcag tttcctgaag
      601 ttaaaacgct cttttgtcgg ttgagcagac agataactac gtccaattat gcggttaatt
      661 aaacctgtaa cttgacccgc tttcgatcga taaggattga atgaagggta aaagtgtgct
      721 ttggcatagt ttataaacga accatcggga atagcagtcc ggcaataatt ttttaaagac
      781 aagcaatatt tttttaaaga caaatagttt gaaagcagtt cagagcgaca ggtatgcaga
      841 aacaatcgtt aactcccaaa tcatcagccg acacggtttt atctgtcctg cccgcattct
      901 gagaaaatat tcagattgcc gtatggataa aagataagcc tatatcaatt ttccggaagc
      961 gaaacgcttc gctgccggtc aggcagtcac ccacagcgtc aatgatatga gcgtagagtt
     1021 ttaatcgtgc ggcacttatc catttttatt gatagcgaat agcctgctcg ttatcaagca
     1081 aaaatttagt aacagttgag aggaagactt atgagtatcc ctggaagcaa tttccctttg
     1141 cgcaataggc gaattatcgc cgttttgagc gccttattat tgctggcggt tgtcggttcg
     1201 acactggcaa gcgccgccga aatttcactt tttggttcgt ttaagcagtt aatcggcttt
     1261 aacgccgaga gttcggttgc cgcctcgccg cagccggttg tgacgacaca gccgctgacg
     1321 attggaacct gcgacacggc aggaccgatt gaggtggaat cgaccggcgg aacgaccgcc
     1381 ccgaccgctt acgcgacatt agcggcagcg tttacggcga ttaacgccgg aacgcatacc
     1441 ggctcaatca acatcgaagt ctgcggcaac acgaccgaaa ccgcgagcgc cactttaacg
     1501 gcgagcggct tggcttcgac ttcttacacc gatgtcacca tccgtcccgt cggcggcgcg
     1561 cgcattatcg aaggctcggt agtcggcgcg attatccgtc ttaacggcgc tgacaatgtg
     1621 accatcgacg gtcgacaggg cggcgcggga acggcgcgcg acctgaccgt ccgcaacaac
     1681 agcaccgctg ccgcgacggc agccgtctgg ctttcgagcg tcgccgccgg caacggcgcg
     1741 gcgagcaaca cggttcgcaa tctcgaactc gccggcggca ccgatac
//
LOCUS       DASWNE010000682         2819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_481823, whole genome shotgun sequence.
ACCESSION   DASWNE010000682 DASWNE010000000
VERSION     DASWNE010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481823"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VGB68_13305"
     CDS             <1..317
                     /locus_tag="VGB68_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050265.1"
                     /translation="RNLNDNPYEFLPAQPGIGKVILSARPQVVPVFIAGLGNDLPKQV
                     LGNWRGGEKVRVWFGEPLELSEFYEKRDSLRTHKEIADFLMTKIGELAEKDKQIFAPE
                     KN"
     gene            complement(314..1480)
                     /locus_tag="VGB68_13310"
     CDS             complement(314..1480)
                     /locus_tag="VGB68_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050266.1"
                     /translation="MTRTIFLLLSTIFIFAFSIKAQTPTPSIVPEVTPYPVMQAPPLK
                     TNWGFDDKTAPEAEARLKKIPDAQHTPEDVEIRIELIGYYDRKETPESKKAAQAHRLW
                     LIRNRPERPYYNELGWWMTDEGLKNPDYIALKEEWLKQVNLKKTNDKIRLGAARFLRQ
                     LDDALAEKLLREGQQINPNLYDYSERLMEIYEYFVVVAVGDYESAKVNGREADIDAAL
                     RKLLAEGEKGLALIAKDENLATGSEHFELFVRLAEVALELNELKKARAFAEAVLNKPK
                     NGDDDDDGWRDTEKAIYYGNTLLGRVALREGNAAKAREHLSASLAFPEESDFELRELK
                     MDLAAEMLARGEKQLVLDYLARAEKLTTDEGNIKIFRRWQRLIKRNAFPCWEDD"
     gene            1590..2549
                     /locus_tag="VGB68_13315"
     CDS             1590..2549
                     /locus_tag="VGB68_13315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEY0050267.1"
                     /translation="MSNNSNLPLVIVNPKSASGSTESRWAQVASDLRAHFGAFQVAFT
                     KKRGDGSSLAKRGAESGRKFIIACGGDGTINEVANGILESGGDIELGVLPSGTGGDFR
                     RTLGMPSGAREAAIGLKTGETKKIDVGRVTFLNHAGRETSRYFLNVASFGLSASIIER
                     VKTKNKFDWLPHDLLRGRATFAVSTLQEVLDLNFVTVRVKIDEKEEKTLNTINFAVAN
                     SRYFGGGMKIAPDACINDGFFDIVNIGDMKTAKILINAYTLYNGSHLNLSEVNHINAK
                     RVEVKSADAQKEIQLETDGELPGKLPATFEIVPEALKVRVPKQ"
     gene            2595..>2819
                     /locus_tag="VGB68_13320"
     CDS             2595..>2819
                     /locus_tag="VGB68_13320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HEY0050268.1"
                     /translation="MLFDELTSKILEAGFEVSKELGAGFLESVYEKALLVALRQKGLN
                     AVSQVPLQVKFRGVIVGEFYADMLVENKVLL"
BASE COUNT          663 a          698 c          756 g          702 t
ORIGIN      
        1 aacgcaattt aaacgataat ccgtatgaat ttctgcccgc gcagcccggc atcggaaaag
       61 taatcctgag cgcccgcccg caggtcgtgc ccgtttttat cgccggtctg ggaaacgatt
      121 tgccgaaaca ggttctggga aactggcgcg gcggcgagaa agttcgcgtc tggttcggcg
      181 agcctctgga gctttccgaa ttttacgaaa aacgcgattc gctgcggacg cacaaggaaa
      241 tcgccgactt tctgatgacg aaaatcggcg agctggcgga aaaggacaaa cagattttcg
      301 cgcccgaaaa aaattaatcg tcttcccaac acggaaacgc gtttcgctta atcagccgtt
      361 gccagcggcg gaagattttg atgttgccct cgtcggtggt cagtttttcc gcgcgcgcca
      421 gataatcgag cacgagttgt ttttcgcctc tggcgagcat ctcggcggct aaatccatct
      481 tcagctctct cagctcgaaa tcgctttctt cgggaaacgc gagcgatgcc gaaagatgtt
      541 ccctggcttt cgccgcgttg ccttcgcgca gcgcgacgcg tccgagcaag gtgttgccgt
      601 aataaatcgc tttctcagta tcccgccagc cgtcgtcgtc gtcgtcgccg tttttcggtt
      661 tattcaaaac cgcttcggca aaggcgcgcg cctttttcag ctcgtttaat tcgagcgcga
      721 cttcggcgag cctgacgaac aattcgaaat gctcgctgcc ggtcgccaga ttttcgtctt
      781 tggcgatgag cgccaagcct ttttcgcctt cggctaaaag ctttctgagc gccgcgtcga
      841 tgtcggcttc cctgccgttg actttggctg attcgtaatc gccgacggcg acgacgacaa
      901 aatactcgta aatttccatt aagcgctcgg aataatcgta aagattcggg ttgatttgct
      961 gaccttccct gagcagtttt tcggcgagag cgtcgtccag ttgacgcaga aagcgcgccg
     1021 cgccgagccg gattttatca ttcgttttct ttaaattgac ctgtttcagc cattcctctt
     1081 tcagcgcgat gtaatccggg ttcttcaagc cttcgtcggt catccaccag ccgagctcgt
     1141 tgtaataagg tctttccggg cgattgcgga tgagccacag gcgatgcgcc tgcgccgcct
     1201 ttttcgattc gggcgtctct ttgcggtcgt aatatccgat cagctcgatt ctgatttcga
     1261 catcctcggg cgtgtgctgc gcgtcgggaa tttttttcag ccgcgcttcg gcttcgggcg
     1321 cggttttatc gtcgaatccc caatttgttt tcagcggcgg agcctgcatc accggatacg
     1381 gagtcacttc gggaacaatc gacggcgtcg gcgtttgcgc cttgatggaa aaagcaaaaa
     1441 tgaagatggt cgaaagcagt aaaaaaatcg tcctggtcat taaagtttcc ttttaattaa
     1501 aaaaaaatct gccgaatttt aacccgaaac gaaacgcttt gtaaaaattc ctttgcgaag
     1561 tcgttaaatt tttcttacac tttggcagaa tgtcaaataa ttccaatctt ccgctggtca
     1621 tcgtcaatcc gaaatcggct tcgggttcga ccgaaagccg ctgggcgcag gtcgcctcgg
     1681 atttgcgcgc gcatttcggc gcgtttcagg tcgcgtttac caaaaagcgc ggcgacggca
     1741 gctcgctggc aaagcgcggc gccgaatcgg ggcgaaaatt tattatcgcc tgcggcggcg
     1801 acggcacgat aaacgaagtg gcaaacggaa ttctggaatc gggcggcgac atcgagctcg
     1861 gcgttttgcc ttcgggaacg ggcggcgatt ttcgccggac gctcggaatg ccgagcggcg
     1921 cgcgcgaagc ggcgataggc ttgaaaaccg gcgagacgaa aaaaatcgat gtcgggcgcg
     1981 tgacgttttt aaatcacgcg gggcgcgaga cttcgcgtta ttttctgaac gtcgccagct
     2041 tcggcttgag cgcgtcgatt atcgagcgcg tcaaaacaaa aaataaattc gactggctgc
     2101 cgcacgattt gctgcgcggc agagcgacgt ttgccgtttc gaccttgcag gaagttctgg
     2161 atttaaattt tgtcacggtg cgcgtaaaaa ttgacgaaaa ggaagaaaaa accctgaaca
     2221 cgataaattt cgccgtcgcc aattcgcgct atttcggcgg cggaatgaaa atcgcgcccg
     2281 acgcgtgcat taacgacggc tttttcgaca tcgtcaatat cggcgatatg aaaacggcga
     2341 aaattttgat aaatgcctac acgctctaca acggctcgca tctgaattta tcggaagtca
     2401 atcatataaa cgccaaacgg gtcgaagtaa aatccgccga cgcgcaaaag gagattcagt
     2461 tggaaaccga cggcgaattg cccggaaagc ttccggcgac attcgagatt gtgcccgaag
     2521 cgttaaaggt aagagttcct aaacaataat tcaaagtagg cttcgcggct tgatgaacag
     2581 gatggacagg ataaatgctt tttgatgaac tgacgagcaa aattttggaa gcgggttttg
     2641 aagtgagtaa agaattaggt gcggggtttc ttgagtcggt ttatgaaaaa gcgttgctgg
     2701 ttgcattaag acaaaaagga ttaaatgcag tttcgcaagt tccgcttcaa gtaaaatttc
     2761 gcggtgttat cgttggtgaa ttttatgccg atatgctggt cgaaaacaaa gttttgtta
//
LOCUS       DASWNE010000683         2194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_374455, whole genome shotgun sequence.
ACCESSION   DASWNE010000683 DASWNE010000000
VERSION     DASWNE010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374455"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VGB68_13325"
     CDS             complement(<1..388)
                     /locus_tag="VGB68_13325"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0050269.1"
                     /translation="MISNQEFLQNSYDAFNKREIETLISQMRPDVKWANGLEGGFVYG
                     RDQAREYWTNQFRDIQPERETLKFETDENKRNIVTVHQIIIDLQGNVLAEATVHQIFT
                     IEDELISLYEIGDTETIQEMIRKTRAS"
     gene            730..1869
                     /locus_tag="VGB68_13330"
     CDS             730..1869
                     /locus_tag="VGB68_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050270.1"
                     /translation="MPKTPQSVELYNSWFMEFAPSAYRLDAGKTLLERNVWGQYATPP
                     KLADEILEYAETLLPPDSKIRFLDPPIGTGAFYSSLLKMFSSKRIERSMGFEKDERVA
                     VECLRLWESKGLDLHINDFTLAEIPQEERDKANLVICNPPYIRHHHLSGDDKARLKSL
                     SKAITDCDFGGLTGYYAYFMAICHVWMAQDAVAGWLIPSEFMDVNYGREIKKYLLSRL
                     TLLRIPRYDPEDVQFDDALVSSSIVWFTNTPPAPDHEIEFTFGGSLKKPEKRKFITTA
                     AASTARKWTILPLTRGKPKAAASACKLSDLFTVKRGLATGANEFFILSSAQVAEHNLP
                     AQFLTPILPSSRYLKDDIVEADSKGNPIPDQKLYLLNCSLPEEKI"
     gene            1964..>2194
                     /locus_tag="VGB68_13335"
     CDS             1964..>2194
                     /locus_tag="VGB68_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050271.1"
                     /translation="MQENRPPAPILCTYMGRNNKSTGKPFRFILNRSNATAANGFLMI
                     YPKWNLFNGDFNNPKFVTFIWRKLNNLSADDIT"
BASE COUNT          600 a          513 c          462 g          619 t
ORIGIN      
        1 tggaagctct tgtttttcga atcatctctt ggatcgtttc ggtatcgccg atttcataaa
       61 gactgattaa ttcatcctca atggtaaaga tttgatgaac tgtcgcctcc gcaagcacat
      121 tgccctgcaa atctatgatg atttgatgaa cggtgacaat attccgcttg ttttcgtccg
      181 tctcaaactt taatgtctcc cgctccggtt gaatatccct aaattgatta gtccaatatt
      241 cccgcgcctg atcgcgtccg taaacgaaac cgccttccaa cccgttcgcc catttcacgt
      301 ccgggcgcat ctgcgagatg agcgtttcaa tctcgcgttt gttaaaagca tcgtatgaat
      361 tttgcagaaa ctcttgattt gaaatcataa ttattcacca tacgctgcga atttcataca
      421 aacaaataag caaccaaaag ccgaacattt gtgaaaacac cgattctttc aattcatctc
      481 aaaaagagtc agtttcttta cttttcctgt tagacaaaat aactgaaatg cctacttaac
      541 cttttttatt agagcaaaac attactgacc aaaaacagta gtttttggtc actctgataa
      601 atcggttaat tatattagta aatatcggcg tccgaaaata atagaaagag tttttggtca
      661 gtgcccgata taaaatccgt tgtctcaaaa acgctcattt tctttactga ctttttattg
      721 ctcaatatta tgccgaaaac ccctcaatcg gtcgagcttt ataatagttg gttcatggaa
      781 ttcgcgccga gcgcttaccg actggacgcc ggaaaaacgt tgctcgagcg taatgtctgg
      841 ggtcagtatg cgactccgcc gaagctggcc gatgagattc tcgaatacgc cgaaacgctt
      901 ctgccgcccg attcgaaaat tcgattcctc gatccgccaa tcggaacggg agcattttac
      961 tcgtcattac tgaagatgtt ttctagtaaa agaatcgaga ggtcaatggg tttcgagaaa
     1021 gatgaaagag ttgctgtgga gtgcctgcgt ctttgggaaa gtaaaggact cgaccttcat
     1081 ataaatgatt ttacgcttgc cgagattccg caagaagaac gggataaagc taatttggtg
     1141 atttgcaatc cgccctatat ccgccatcat catctttcgg gcgatgacaa agcacgatta
     1201 aagtcgctat cgaaggcgat taccgattgc gatttcggcg gtctgaccgg atactacgct
     1261 tacttcatgg cgatctgcca cgtctggatg gcgcaagacg cagtagctgg ttggctcatt
     1321 ccgagcgaat tcatggacgt gaattacggc cgcgagatca agaaatatct attgagccgg
     1381 ctcactctgc tgagaattcc ccgttacgat ccggaagacg tccagttcga tgacgcgctc
     1441 gtttcctctt cgatcgtctg gtttacaaac acgccgcctg cgccggatca cgaaatcgaa
     1501 tttaccttcg gcggatcatt aaaaaaacct gagaagcgaa agtttattac gacagctgct
     1561 gcatccacag cccgcaaatg gacgatcctg ccgttaacga gaggtaaacc gaaagctgcc
     1621 gccagcgcct gtaaattgtc cgatctattt acggttaagc gcggattagc aaccggcgcc
     1681 aatgaattct ttattttatc gtcggcgcaa gttgccgaac ataacctgcc ggcgcagttt
     1741 ctaacgccga ttctaccaag ctcgcgctat cttaaagatg atattgtcga agcggattct
     1801 aaaggcaatc cgattcctga tcaaaagtta tatttgctca actgctccct gccggaagaa
     1861 aagatctagt cgggttttcc cttcactttg gaaatactta caaaaaggcg ttgaaatggg
     1921 cgttagtgaa agatatttgt gcaaaagccg cactctctgg tatttgcagg aaaatcgtcc
     1981 gcccgcgccg attctctgta cttacatggg acgcaataac aaatcaaccg ggaagccttt
     2041 ccgatttatc ttaaatcgct caaatgcgac ggctgccaat ggttttctaa tgatttatcc
     2101 taagtggaat ctgtttaatg gtgatttcaa taatccgaaa ttcgttactt tcatttggcg
     2161 aaagctcaat aatttatctg ccgatgacat aact
//
LOCUS       DASWNE010000684         1927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_127709, whole genome shotgun sequence.
ACCESSION   DASWNE010000684 DASWNE010000000
VERSION     DASWNE010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127709"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..837)
                     /locus_tag="VGB68_13340"
     CDS             complement(79..837)
                     /locus_tag="VGB68_13340"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEY0050272.1"
                     /translation="MRRFYAPPENFKGGEITLDFEQTKHLRDVLRLRAGENINIFDGE
                     GKEFSCAIQEIGKRETALKIIAEVNPRAPESVLDLTLAVALLKGEKFDLVLQKAVELG
                     VTRLVPLQTRRADVKLKDAGEIEKKLERWRKIVFEAAKQCGRAKLMQIENPQGFEKFI
                     EKKFIEKSSPPNSGAEIRVLFSERGGEKFSDLQAGKKIVAVIGSEGGWEDAEIELARA
                     NEFQIITLGGRILRAETAAIVVSAVLQNHFGDLR"
     gene            complement(943..1518)
                     /locus_tag="VGB68_13345"
     CDS             complement(943..1518)
                     /locus_tag="VGB68_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050273.1"
                     /translation="MYKSRTKNDLIIEVWEALDCESVGAKELVAIETAVRERFGQSAV
                     DSPMKMARLLADEGAELRHAEIMQLDVERRLESPYDAMFRNILKFSDFKQALVSIRNL
                     ENLRRKFQAENDKEGLRLVRETARRGKSRAQMIAGNEKVEESKRAEKAEIAEWFTIWL
                     QSPEIFENWVGLRQNSKDFKDRFRQDSGKSE"
     gene            complement(1665..>1927)
                     /locus_tag="VGB68_13350"
     CDS             complement(1665..>1927)
                     /locus_tag="VGB68_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050274.1"
                     /translation="PFMPETERAEIVSALKFVDYVTIFPEATVTELIRAVRPDFHAKG
                     TDYTTETVPEREIVREYGGRVAIVGDPKDHSSTDLIGKVAGS"
BASE COUNT          420 a          523 c          465 g          519 t
ORIGIN      
        1 gataaagctg aaatgcagaa gcgcggagac gccttttacc gcccggtgaa aaagcaaaat
       61 cccgataatt aagcgttttt agcgtaaatc gccgaaatgg ttttgcaaaa cggcggaaac
      121 gacgattgcc gccgtttccg cccgcaaaat tcttccgccg agcgtgatga tttgaaattc
      181 gtttgcccgc gccagctcga tttccgcgtc ttcccaaccg ccttccgaac cgatgacggc
      241 aacgattttt tttcctgcct ggaggtcgga aaatttttcg ccgccgcgct cggaaaataa
      301 aacgcggatt tcagcgccgg aattcggcgg cgacgatttt tcgataaatt tcttttcgat
      361 gaatttctca aagccctgcg gattttcaat ctgcatcagt ttggcgcgcc cgcattgttt
      421 cgcggcttcg aaaacgattt tgcgccagcg ttcgagcttt ttttcaatct ctccggcgtc
      481 tttcagcttt acgtcggcgc gtctggtttg aagcgggacg agccgcgtga cgcccagttc
      541 gaccgctttc tgcaagacca aatcaaactt ttcgcctttt aaaagcgcga cggcgagcgt
      601 caagtccagc acggattccg gcgcgcgcgg atttacttcg gcgatgattt taagagccgt
      661 ctcgcgcttt ccgatttcct gaatcgcgca cgaaaattct tttccttcgc cgtcgaaaat
      721 gtttatgttt tcgcccgcgc gcaggcgcag aacatcgcgc agatgtttgg tctgttcaaa
      781 atcgagcgtg atttcgccgc ctttaaaatt ttccggcgga gcgtagaaac gtcgcattgc
      841 aaaagaaagg gaataagtga ataagtgaat aagcgaatag gtgttcgggc gcagcatttc
      901 gcatttttac ttaatcacct gaacacttaa acacctattc acttattcgc tcttcccgct
      961 atcctgtctg aatctatctt tgaaatcctt ggaattttgc cgaagtccga cccaattttc
     1021 aaaaatttcg ggcgattgca gccaaatcgt aaaccactcg gcgatttccg ctttttcggc
     1081 gcgtttgctc tcctcgactt tttcgtttcc ggcaatcatc tgcgcgcggc tttttccgcg
     1141 gcgggcggtt tcgcgcacca gccgcaagcc ttccttatcg ttttccgcct gaaatttgcg
     1201 gcgcagattt tcgaggttgc gaatcgagac gagcgcttgt ttgaaatcgg aaaatttcag
     1261 gatgttgcga aacatcgcgt cgtacggcga ttccagacgc cgctcgacgt cgagctgcat
     1321 aatctcggcg tggcgcagct ccgcgccttc gtccgccagc aggcgcgcca ttttcatcgg
     1381 gctgtcgacg gcgctctgcc cgaaacgctc gcgcaccgcc gtttcaatcg caactaattc
     1441 tttcgcgccg accgattcgc aatcgagtgc ttcccagact tcaataatca aatcattttt
     1501 cgtgcgcgat ttatacataa tagttaacag gaagttgcag atagttgcag atagttgcag
     1561 atagttgcag atagttaaga gaactgtcga agattgaaac tttgaactgt ttttaatctc
     1621 ttccaacttc ctgcaactat ctgcgacttc ccgtcaactt tctgtcaact tcccgcaact
     1681 ttcccaatca aatcggttga cgaatggtct ttcgggtcgc cgacgattgc gacgcgaccg
     1741 ccgtattcgc gcacgatttc gcgttcggga acggtttcgg tcgtgtaatc cgtgcccttc
     1801 gcgtgaaagt ccgggcggac ggcgcgaatc aattccgtca cggtcgcttc gggaaaaatc
     1861 gtcacgtaat cgacgaattt cagcgccgaa acgatttcgg cgcgttccgt ttccggcata
     1921 aacgggc
//
LOCUS       DASWNE010000685         7974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_234343, whole genome shotgun sequence.
ACCESSION   DASWNE010000685 DASWNE010000000
VERSION     DASWNE010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7974
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234343"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..500)
                     /locus_tag="VGB68_13355"
     CDS             complement(27..500)
                     /locus_tag="VGB68_13355"
                     /inference="COORDINATES: protein motif:HMM:NF025734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HEY0050275.1"
                     /translation="MKRILKIIVIVLFTGFIVIQFIRPDMTNPPVVAEQTLEASTAIP
                     ENVKTILSRSCADCHTHNTLYPWYARISPFSWGLAFHIDDGRAHLNFSEWAKLEDRRK
                     KRKLEEVCEQVETREMPLPSYLWIHADAAMSDEEIRLLCDWTKTEIAKFSSGESK"
     gene            776..1240
                     /locus_tag="VGB68_13360"
     CDS             776..1240
                     /locus_tag="VGB68_13360"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEY0050276.1"
                     /translation="MENFSLFQDKHFPGETTTAFGERGERLALQYLLKKNYRVVCGNF
                     KTPVGRNRLGAIITGEIDLIAYDEAFLCFIEVKTRSSDEFASPLAAVDLRKQRQIIRA
                     ARMYRRIFHLQAAKIRYDVVSIVLKKDTKPEIELFKNYFSESRFEKKFWLED"
     gene            1432..3804
                     /locus_tag="VGB68_13365"
     CDS             1432..3804
                     /locus_tag="VGB68_13365"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY0050277.1"
                     /translation="MLKSLLVVLTVGASFLTFTAMAGFAQSKDSMPPLIDREIFFGNP
                     EISGAQISPDGRFISFIKPYKNVRNIWVKGVSEPFEKARVVTAEAKRPIPNYFWSHDG
                     KYILFVKDNDGDENFNVYAVNPAEAPASGADVPAARNLTDAKKVRAFIYNVPESDPDA
                     IYVGLNDREPAWHDLYKVKISTGERTLVRKNTDRLTGWVFDNKDQLRLATRSTPSGET
                     EILRVDADGKFTKIYSCGVFESCAPVAFHKDDKRVYMQTNKGDVDLIRLVLFDPEGGK
                     EEFVEEDPLKRVDFGGLYISDRTKEIVLTSYTDDKTRVYFKDKNFEKDYNTIKKQLGD
                     REISFNSSTKDENLWIIASFSDVDPGTVWLYDRRAKNLSTLYQVREKIPREALAKMQP
                     IRYKSSDGLEIPAYLTLPKGVEPKNLPLVVVPHGGPWGRDTWGYNSFAQFLANRGYAV
                     LMPNFRASTGFGKKFLNAGNKQWGQLMQDDITWGVKHLVAQGVADPKRVGIMGGSYGG
                     YATLAGVTYTPDLYAAAVAIVAPSNLITLLESIPPYWEAGKIIFYERMGNPNTPEGKA
                     LLLKQSPLTHADKIKTPLLVVQGANDPRVNKRESDQIVVALRDRGYPVEYLLAPDEGH
                     GFARPVNNMAMFARGEEFLAKYLGGRFQSGGTPEVMQRLKEITVDVKTVTVANKSGMN
                     VTSNLASAIKVAGKWNLTANVGGDVVEVVLDLKQTGADFTGTVSSMHGGGTVENGKVN
                     GTSLTGKMKVEIQGQPLEIQLEGKVEGDKMTGTMSGAGLPQITFTATKAP"
     gene            complement(3908..4819)
                     /locus_tag="VGB68_13370"
     CDS             complement(3908..4819)
                     /locus_tag="VGB68_13370"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HEY0050278.1"
                     /translation="MKNLIPAFISALLMTISVFGQANISEANYRIYDRQGNASSMEKI
                     VDSLKDVDVVFLGENHDDATAHYLQNEIFRRAFEKHGKERAIALSLEMFERDVQTVVD
                     EYLRDLISENHFLLSARAWGNYKTDYKPLLEFAKTGKLEVIAANAPRRYVNMVSRSGR
                     ESLNRLSPEARRWLAPLPYGEASDAYAKKFSALMGQNSDSATPVKHSPMLNAQALWDA
                     TMAFSIAEYLKRAKNPLVVHLNGAFHTENRLGTPEHLLKYSPKTRFLVVTMRSEDNFA
                     NFDKTKHADLGDFVILTDSKVPRSSQR"
     gene            5269..5964
                     /locus_tag="VGB68_13375"
     CDS             5269..5964
                     /locus_tag="VGB68_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEY0050279.1"
                     /translation="MNNQGSGSGGSGRGTFGEEASAIGDRVSGNVKDAYGAVTGNESV
                     EREGEAQANYGAQRQSNNRILTGMFRDRESAERAYNSVTSRGYTNDDVNLMMTDETRS
                     KYFSDADAGDEGLGSKALEGAGAGSAIGGTLGAIIGGIAAIGTNVLLPGLGLVVAGPL
                     AAALAGAGAGGLTGGLVGALVGSGIPEDRAKEYEEGVKSGGIVMGVNPRSDEDAEYFE
                     NEWRNHRGENIYR"
     gene            complement(6217..6819)
                     /locus_tag="VGB68_13380"
     CDS             complement(6217..6819)
                     /locus_tag="VGB68_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050280.1"
                     /translation="MSNLKKSIRLALILVFSFLIGGEGVKAQFRGDQAVINEAQRITK
                     DAFTLSTRTRQGARIYAVRQPNAKMLAAIDQGLDDLFDAAERNGYRRRLNYSDYVIFI
                     ANADRQVNRDGQYSPDIAVPTGQYAGSVYDQGGFIYAAGMVIGYNPLAFVIAEHDRDY
                     RRVSEAVRNEGEHLVLYHNDRRRYNATLDHSQGGGHPILQ"
     gene            complement(6938..7894)
                     /locus_tag="VGB68_13385"
     CDS             complement(6938..7894)
                     /locus_tag="VGB68_13385"
                     /inference="COORDINATES: protein motif:HMM:NF044507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050281.1"
                     /translation="MSVYENFAPIDLEKINTYELASRPSKVTVRDFSEPVGANDSIKN
                     FLEKLPNILAVQSLREIARQIRRAAELNKPIVWGIGGHVVKTGLAPVLIDLLRQGYVS
                     AIAANGSVLVHDTEIAMVGFTSEDVDASLGKGDFGAARETGEILNEAAKSGATDEIGL
                     GEAMGRAVSALNPPNAEVSILCQAYQNKIPFTAHLAIGADIGHFHASCDGAALGKTSH
                     TDFRLFCSIVKEINGGGVYLNLGSAVILPEIFLKAVTVVRNLGYPLADFTTANFDFIQ
                     GYRPNTNVVRRPTAGGAGRGFSVTGHHEIMIPLLAAQILCGK"
BASE COUNT         2042 a         1975 c         2059 g         1898 t
ORIGIN      
        1 ctctcataac tggcaacttg gtttaattat ttactttcgc cgcttgaaaa tttcgcgatt
       61 tccgttttcg tccagtcgca gagaaggcgg atttcctcgt ccgacattgc cgcgtcggcg
      121 tgaatccaga gatacgacgg cagaggcatc tcgcgcgtct cgacctgctc gcagacttct
      181 tcgagcttgc gttttttgcg ccggtcttcg agcttcgccc actcggaaaa attcagatga
      241 gcgcgcccgt cgtcaatatg aaacgccaag ccccacgaaa acggcgaaat ccgtgcatac
      301 cacggataga gcgtgttgtg cgtgtgacaa tcggcgcacg agcggctcaa aatcgttttc
      361 acgttttcgg gaatcgcggt tgacgcttcc agcgtttgct cggcgaccac cggcggattc
      421 gtcatatcgg gacgaataaa ttgaatgacg atgaaaccgg tgaaaagaac gatgacgatg
      481 atttttaaaa tgcgtttcat tttatttcaa gccggagatt tctccgttaa tatactgtca
      541 tcgattagcc gcccgaaata caagcgcgac cgtaaaaatg tctacaggct ttgcggcttg
      601 atttcgtgtt tttgcggcgc gctgaaatta aagactttcg gcagatttgc ggtgttttct
      661 ccggtcgtcg ttttcggctg tcaaacggcg attaaagccg tcaaaggcaa aacggcgaga
      721 acggcgaaaa aaccttgtat ttgtttttta ttcatatata ttacccttat tcggaatgga
      781 aaatttttct ttgtttcagg ataaacactt tcccggagaa acgacaaccg cgttcggcga
      841 gcgcggcgag cggctggcgc tgcaatatct tttaaagaaa aactatcgcg tcgtctgcgg
      901 gaatttcaaa actccggtcg ggcgcaaccg gctcggcgcg attatcacgg gcgagattga
      961 tttaatcgcc tatgacgaag ctttcctgtg ctttatcgaa gtcaaaacgc gctcgtccga
     1021 cgaattcgct tcgccgctcg cggcggtcga tttgcgaaag caaagacaga ttatccgcgc
     1081 ggcgcgaatg tatcggcgga tttttcattt acaagcggcg aaaatccgtt atgatgtcgt
     1141 cagcatcgtt ttaaaaaaag acacaaaacc cgaaatcgag ctttttaaga attatttcag
     1201 cgaaagccgg tttgagaaaa agttttggct ggaagattaa tcttcaaaag ttttgaatat
     1261 cgaaaagttt tgaatatcga actttaaatg aatcgttaac cgggcagaat caaaaccgga
     1321 aaattcaaaa cccggaattc ataattgaac aaaggcggaa cattcgattt tcggtcgccg
     1381 tagcttaacg gtaataatct ataaatttct ttcgaggtgt acaaaaaaaa aatgcttaaa
     1441 agtctactgg ttgttttgac tgtcggtgcg agttttttaa ctttcacggc tatggcgggc
     1501 tttgcgcaga gcaaagattc gatgccgccg ctgattgacc gcgaaatctt tttcggaaat
     1561 ccggaaatct cgggcgcgca aatctcgccc gacggcagat ttatctcctt tatcaagccc
     1621 tacaaaaatg tgcggaacat ctgggtcaaa ggcgtcagcg agccgtttga aaaagcgcgc
     1681 gtcgtgacgg cggaagccaa gcgcccgatt ccgaattatt tctggagtca cgacggaaaa
     1741 tacattctgt tcgtcaagga caacgacggc gacgaaaatt tcaacgtcta cgccgtcaat
     1801 ccggcggaag ctccggcaag cggcgcggac gtgcccgcgg cgagaaatct gaccgacgcg
     1861 aaaaaagttc gcgcctttat ctacaacgtt cccgaaagcg acccggacgc gatttacgtc
     1921 ggcttaaacg accgcgaacc ggcttggcac gacctttaca aagtcaaaat ctcgaccggc
     1981 gagcgcactc tggttcgcaa aaacaccgac cgtctgaccg gttgggtttt cgataacaaa
     2041 gaccagttgc gcctcgcgac gcgctcgacg ccgagcggcg agacggaaat tctgcgcgtc
     2101 gatgcggacg ggaaatttac gaaaatttat tcgtgcggcg tgtttgaatc gtgcgcgccg
     2161 gtcgcctttc ataaagacga caagcgcgtt tatatgcaga ccaataaggg cgacgtcgat
     2221 ttgattcgcc tcgttctgtt cgacccggaa ggcggcaagg aagaattcgt cgaggaagac
     2281 ccgttgaaac gcgtcgattt cggcggtttg tatatttccg acagaacaaa agagattgtt
     2341 ctgaccagct acaccgacga caaaacgcgc gtttatttca aagacaagaa ctttgaaaaa
     2401 gactacaaca cgattaaaaa acaactcggc gaccgcgaaa tcagctttaa ttcttcgacc
     2461 aaagacgaaa atctatggat tatcgcctcg ttcagcgacg ttgaccccgg aacggtctgg
     2521 ctctacgacc ggcgggcgaa aaatctctcg acgctttacc aggtgcgcga aaaaattccg
     2581 cgcgaagctt tggcgaaaat gcagccgatt cgatacaaat cgtcggacgg cttggaaatt
     2641 cccgcttatc tgactttgcc gaaaggcgtc gagccgaaaa atctgccgct cgtcgtcgtg
     2701 ccgcacggcg gaccttgggg acgcgatacg tggggttaca attctttcgc gcaatttctg
     2761 gcaaatcgcg gctacgccgt cctgatgccg aatttccgcg cttcgaccgg cttcggcaaa
     2821 aaatttctga acgcgggcaa taaacaatgg ggacagctga tgcaggacga catcacgtgg
     2881 ggcgtcaaac acctggttgc gcagggcgtc gccgacccga aacgcgtcgg gattatgggc
     2941 ggcagttacg gcggctacgc gacgctcgcc ggcgtcacct acacgccgga tttatacgcg
     3001 gcggcggtcg ccatcgtcgc gccgtcgaat ttgattacgc tgctcgaatc gattccgccg
     3061 tactgggaag cgggcaaaat tattttttat gaaagaatgg gcaatccgaa cacgcccgaa
     3121 ggcaaagcgc tgcttttgaa acagtcgcct ttgacgcacg ccgacaaaat caagacgccg
     3181 cttctggtcg tgcagggcgc aaacgacccg cgcgtcaaca agcgcgagtc cgaccagatt
     3241 gtcgtcgcct tgcgcgaccg cggttatccg gtcgaatacc tgctcgcgcc ggatgaagga
     3301 cacggtttcg cgcgtccggt caacaatatg gcgatgtttg cgcgcggcga agaatttctg
     3361 gcgaaatatc tgggcggtcg tttccagtcg ggcggcacgc cggaagtgat gcagcgcctg
     3421 aaggaaataa cggttgacgt gaagaccgtg accgtggcga acaaatcggg catgaatgtc
     3481 acgtctaatc tcgcgtcggc aattaaggtc gccggaaaat ggaatttgac cgcaaacgtc
     3541 ggcggcgacg tcgtcgaagt ggtgctcgat ttgaaacaga cgggcgcaga ttttaccggc
     3601 acggtctctt cgatgcacgg gggcggaacg gtcgaaaacg gcaaggtcaa cggaaccagt
     3661 ctgaccggaa agatgaaagt cgaaattcaa ggtcagccgc tggagattca attggaagga
     3721 aaggtcgaag gcgataaaat gaccggcacg atgtccggcg cgggacttcc gcagatcacc
     3781 tttacggcga cgaaagcccc ataaaggtaa aaagcgaaag agcggaaaag cgaaagagcg
     3841 aaaaagtcga gttgagtttt tctcagctta ctttttcact ctttcacttt ttcacgtttt
     3901 aactttatta tctctgactg ctgcgcggaa ctttcgagtc ggttaaaatc acgaaatcgc
     3961 ctaaatccgc gtgttttgtc ttatcgaaat tcgcgaaatt atcttccgaa cgcatcgtca
     4021 cgactaaaaa tctcgttttg ggcgaatatt ttaaaagatg ctcgggcgtt cccagacggt
     4081 tttcggtgtg aaaagcgccg ttcagatgga cgaccagagg atttttcgcg cgctttaaat
     4141 attcggcaat cgaaaaagcc atcgtcgcgt cccagagcgc ttgcgcgttg agcatcgggc
     4201 tgtgtttgac gggcgtcgcc gaatcggaat tttgtcccat cagggcgcta aattttttgg
     4261 cgtaggcgtc agacgcttcg ccgtacggca aaggcgcgag ccagcggcgc gcttcgggcg
     4321 aaagccgatt taaagattcg cgaccgctgc gcgaaaccat attgacataa cggcgcggcg
     4381 cgttggcggc gatgacttcg agtttgccgg ttttggcaaa ttcgagaagc ggcttgtagt
     4441 cggttttgta atttccccag gcgcgcgcgc tgagcagaaa atggttttcc gaaatcaaat
     4501 ccctcagata ttcgtcaacg accgtttgca cgtcgcgctc gaacatttcc agcgaaaggg
     4561 ctattgcgcg ttctttgccg tgtttttcaa aggcgcgtcg aaaaatttca ttttgcagat
     4621 aatgcgccgt cgcgtcgtcg tgattttcgc cgagaaaaac gacgtccaca tccttcagcg
     4681 aatcgacaat cttttccata ctcgaggcat ttccctgtct gtcgtaaatg cggtaattcg
     4741 cttcgctgat atttgcttgt ccgaaaactg aaatcgtcat taaaagtgcc gaaataaaag
     4801 ccggtattaa atttttcata aattgaatcc gaaaaagaca aatcacagat aaacacattt
     4861 ccgccgttgt ttcaaccgat tgtaattatc gtgctctgca caaaaatcaa gaaaagcgat
     4921 gaaaattcgg gtcgcagact gtaaattata aattataaat tggttgacca aagatattga
     4981 atatcaattt agaatttgta atttacaatc tgcgattttc ccttttcaat tgctttgcgg
     5041 agccgtgact atgtcttaaa ttattacaaa atatttgtcc gtaaatatta cgaaagataa
     5101 ttttaattcg cgccgggatt gatttcaagc cgcactaaat ccggctaata aacgatttat
     5161 tcatacaatt tattaattgg cacgatgctt gcactaaagc tttcaagtta tcaggctgac
     5221 aattttcagc agactcgaaa aattctaacg aacgaaggga gtttcaaaat gaacaatcaa
     5281 ggatcaggat cgggaggctc aggtagaggc acatttggcg aagaagcatc ggcaatcggc
     5341 gaccgcgtca gcggcaacgt gaaagatgca tacggcgcag taactggcaa cgaatcggtc
     5401 gaacgcgaag gcgaagcgca agccaattac ggcgcgcagc gccagtcaaa caatcgtatt
     5461 ttaaccggga tgtttcgcga ccgcgaaagc gccgagcgcg catataattc ggttaccagt
     5521 cgcggctata caaatgacga cgtcaatctg atgatgaccg acgagacgcg cagcaaatat
     5581 ttttcggatg ctgacgccgg tgacgaaggt ctcggctcga aagcgctgga aggcgctggc
     5641 gcgggttcgg caatcggcgg aactttaggc gcaattatcg gcggcatcgc ggcaatcgga
     5701 acgaacgttc tgcttccggg attaggattg gtcgtcgccg gaccgctcgc cgcggcattg
     5761 gctggcgcgg gcgcgggcgg cttgaccggc ggattggtcg gcgcattggt cggctcggga
     5821 attcccgaag accgcgctaa agagtatgaa gaaggcgtta aaagcggcgg tatcgtgatg
     5881 ggcgtcaacc cgcgcagcga cgaagatgcc gaatattttg agaatgaatg gcgcaaccat
     5941 cgcggtgaaa acatttaccg ctagtttgta aaattacatt ctcctgtcag aaaagacctc
     6001 gccgtaaaaa gcgaggtctt ttcgtattct aaaatcaatt aaagaactct aaatccgagc
     6061 gggctcgttt aatcgggaat gggacggtga ttttttaaga taaattaaga gcggttgaga
     6121 ttaatcaggg aaacttccac caatcccgac caatcctaat caatcagcgc ccattttttt
     6181 gcctgctttc acgacacaca tcttcagcct ttcaatttac tgcaaaatcg ggtgtccgcc
     6241 gccctggctg tggtctaatg tcgcgttgta gcggcgacgg tcgttgtgat aaagaaccag
     6301 atgctcgcct tcgttgcgca cggcttctga aactcgccgg taatctctgt cgtgttcggc
     6361 gatgacaaac gccagcggat tatagccaat caccattccc gccgcgtaaa taaagccgcc
     6421 ctgatcataa acgctgcccg cgtattgccc ggtcggaacg gcgatgtccg gcgaatattg
     6481 accgtcgcga ttcacctggc gatcggcgtt ggcgataaaa atcacgtaat ccgaataatt
     6541 caagcgtctt cgataaccgt ttctttcggc ggcgtcgaac aaatcatcca agccctggtc
     6601 aatcgccgcg agcattttcg cgttcggctg gcggacggcg taaattcgcg cgccctgccg
     6661 cgttcgggtc gaaagcgtaa aagcatcttt cgtaattcgc tgcgcttcgt tgataacggc
     6721 ttggtcgccg cgaaattgag ctttgacgcc ttcgccgcca attaaaaaac taaaaactaa
     6781 aattaatgca agccggattg atttttttaa attagacata atttgactct ccgccggttc
     6841 attttgcaaa cctcgtgcca gctcgattta ccgtaattta ttggctttta ttaaaaaatc
     6901 cgcacttttt ggagagtagg caagacgata tgcgtcatca cttgccgcaa agaatctgcg
     6961 cggcgagcaa cggaatcata atttcgtgat gtccggtgac ggaaaatccg cgccccgcgc
     7021 cgcccgccgt cggacgacga acgacattcg tattcggtcg atagccctgt ataaaatcga
     7081 aatttgcggt cgtaaaatcg gcgagcggat aacctaaatt tctgacgacc gtcacggctt
     7141 tgagaaaaat ttccggcaga atgaccgccg agccgagatt cagataaacg ccgccgccgt
     7201 ttatttcctt aactatcgaa caaaaaagtc gaaaatcggt gtgcgaagtt ttgccgagcg
     7261 ccgcgccgtc gcaagaggcg tgaaaatgcc cgatgtccgc gccgatcgcc agatgcgcgg
     7321 tgaaaggaat tttgttttga taagcctgac aaagtattga aacttctgcg ttcggcggat
     7381 tcagcgcgga aactgccctg cccatcgctt cgcccagacc gatttcgtcg gtcgcgccgc
     7441 ttttcgccgc ttcattcaaa atctcgcccg tttcgcgcgc cgcgccgaaa tcgcctttgc
     7501 ccaggctggc gtcaacgtct tcgctggtga aaccgaccat cgcaatttcc gtatcgtgaa
     7561 ccagaaccga gccgtttgca gcaatagcgg agacgtatcc ttgccgcagc aaatcaatca
     7621 aaacgggcgc gagtccggtt ttgacgacgt gaccgccgat gccccagaca atcggtttat
     7681 ttagctctgc ggcgcgccgg atttgccggg cgatttcgcg cagagattga acggcgagaa
     7741 tattcgggag cttttcaaga aaattcttta tggaatcgtt tgcgccgaca ggctcggaaa
     7801 aatcccggac ggttactttg ctcggacgcg aagcaagctc gtaagtattg attttctcca
     7861 aatctatcgg cgcaaaattt tcgtaaacgg acatagcaga ttctgccgat tataacacgc
     7921 tttagctctg cccggtgaaa gtataagtca gcagtcccgt cgtttccgcg ttcg
//
LOCUS       DASWNE010000686         2140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_692995, whole genome shotgun sequence.
ACCESSION   DASWNE010000686 DASWNE010000000
VERSION     DASWNE010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692995"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..158)
                     /locus_tag="VGB68_13390"
     CDS             complement(<1..158)
                     /locus_tag="VGB68_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY0050282.1"
                     /translation="MSKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITKVMSKHNPKMI
                     VRAFDSID"
     gene            complement(237..>2140)
                     /gene="fusA"
                     /locus_tag="VGB68_13395"
     CDS             complement(237..>2140)
                     /gene="fusA"
                     /locus_tag="VGB68_13395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY0050283.1"
                     /translation="ATTCFWKRNNFEHRINIIDTPGHVDFTMEVERSLRVLDGAVAVF
                     DGVAGVEPQSETVWRQADKYGVPRLCFINKLDRAGASFERSFDSILKRLGANAVALQI
                     PIGLEDQLKGVVDLITMKGLIWNDETKGAQYETTDIPAELVDEAKEWREKLVEAVSSI
                     DDDLMMKYLEGEEISEAEIRKALRQGTLELKIVPVVTGSAFKNKGVQTLLDAVVDYLP
                     SPLDVPPIEGINPKTDETETREAAGSAPFSGLVFKLMADKHLGQLAFVRIYSGTVNSG
                     SYVYNTIKGNKERVGRLMLMHANKREDVDSAAAGEIVAIGGMKNVTTGDTISDENKQI
                     ILESMEFPDPVVRVAVEPKTRQDQDKMGIALNRLAQEDPSFQVSTDHETGQTIIAGMG
                     ELHLEIIVDRMKREFGVEANVGKPQVAYRETITIPAPGKEIFKKQSGGRGQFAHVELE
                     VEPAPGEGFVFEDKITGGAIPRQFIKPTMEGIRDAMARGFLAGYELVDIKATLNFGSY
                     HEVDSDERSFHIAGSLAFQDAIKKAKPVLLEPIMRIEVVTPEEYMGSVNGDLNRRRGQ
                     IEGMTPRPGGVQVVTAFVPLSEMFGYTTDLRSATQGRATSTMHFERYDQAPRNVAEEV
                     IAKVKGVTT"
BASE COUNT          430 a          606 c          553 g          551 t
ORIGIN      
        1 ttgtcgatcg aatcgaacgc ccgcacaatc atcttcggat tatgcttgct catcactttc
       61 gtgatcgccg ccgtcaatgt cgttttcccg tggtcgacgt gcccgattgt gccgatgttt
      121 acgtgcggct tgcttctgtc aaatttctct ttgctcataa ctttaagctc tttgctcccc
      181 taaatttatg agaaggacga aagattttga gcctatcctt tcgccttcac ttttaattaa
      241 gtagtaacgc cttttacttt cgcgataact tcttcggcaa cgttacgcgg cgcttggtcg
      301 taacgttcaa aatgcatcgt cgaagtcgcg cgtccctgcg tcgcgcttct taaatcggtc
      361 gtgtaaccga acatttccga aagcggaacg aacgcggtca ccacctgaac gccgcccggg
      421 cgcggagtca ttccttcaat ctgcccgcga cgacgattta aatcgccgtt gaccgaaccc
      481 atatattctt cgggcgtgac gacctcgatt cgcataatcg gttcgagcag aacgggtttc
      541 gcttttttga tggcgtcctg aaacgccaag cttccggcaa tatggaaaga gcgctcgtcc
      601 gagtcgactt cgtgatacga gccgaaattc aaagtggctt tgatatcgac cagctcgtag
      661 cccgccagaa atccgcgtgc catcgcgtcc ctgatgcctt ccatcgtcgg cttgataaac
      721 tggcgcggaa tcgcgccgcc ggtgattttg tcttcaaaga caaagccttc gcccggcgcg
      781 ggttcgactt cgagctcgac gtgcgcgaac tgaccgcgac cgcccgactg cttcttgaag
      841 atttctttgc ccggcgcggg aatcgtaatc gtctcgcggt aagccacctg cggtttaccg
      901 acattggctt cgacgccgaa ttcgcgcttc atacggtcga cgataatttc aaggtgcagc
      961 tcgcccattc cggcgatgat ggtctgaccc gtttcgtggt cggtcgaaac ttggaaactg
     1021 gggtcttcct gtgccagacg attgagcgcg atgcccattt tgtcctggtc ctgacgagtt
     1081 ttcggttcga ccgcgacgcg cacgaccgga tcgggaaatt ccatcgattc gagaataatc
     1141 tgtttgttct cgtcggaaat cgtgtcgccg gtcgtgacgt ttttcatacc gccgatggcg
     1201 acaatttcgc cagccgccgc cgaatcgacg tcctcgcgct tgttggcgtg catcagcatc
     1261 aggcgaccga cgcgctcttt gtttccttta atcgtattgt agacgtaaga gccggagttg
     1321 accgttcccg aataaatacg cacgaaagcg agctgtccga gatgcttgtc cgccattaat
     1381 ttgaaaacca gacccgaaaa cggcgccgaa cctgcggctt cgcgcgtttc ggtttcgtcg
     1441 gttttcggat tgatgccctc gatgggggga acatccagcg ggctcggcaa ataatcgact
     1501 accgcgtcga gcagcgtctg cacgccttta tttttgaaag ccgagccggt gacgaccgga
     1561 acgattttca gttccagcgt tccctgacga agagctttgc gaatttccgc ttcgctgatt
     1621 tcttcgcctt ccagatattt catcatcaaa tcgtcgtcaa tcgaggaaac ggcttcgacc
     1681 agtttttcgc gccattcttt ggcttcatca accaattccg ccggaatatc cgtcgtttcg
     1741 tattgcgcgc ccttggtttc gtcgttccaa atcagacctt tcatcgtgat cagatcaacg
     1801 acgcctttca gctggtcttc caaaccgatc gggatttgca gcgcgacggc gttggcgccg
     1861 aggcgtttta aaatcgaatc gaacgagcgt tcgaacgaag cgccggcgcg gtctaattta
     1921 ttgataaagc agagacgcgg aacgccgtat ttgtcggctt gacgccagac ggtttccgat
     1981 tgcggctcaa cgccggcgac gccgtcgaaa acggcgaccg cgccgtccag aacgcgcaga
     2041 gagcgttcga cttccatcgt gaaatcgacg tgtcccggcg tgtcgataat attgattcgg
     2101 tgttcgaagt tgttgcgctt ccagaaacag gtcgtcgccg
//
LOCUS       DASWNE010000687         5840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_58947, whole genome shotgun sequence.
ACCESSION   DASWNE010000687 DASWNE010000000
VERSION     DASWNE010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5840
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58947"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..1117)
                     /locus_tag="VGB68_13400"
     CDS             complement(89..1117)
                     /locus_tag="VGB68_13400"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0050284.1"
                     /translation="MPKASSFSRFTRGVRHTVRAFASTKHPVLVHIVPMRRCNLACTY
                     CNEYDKTSEPVAIATMLKRIDKLAEFGTSVITISGGEPMLHPEIYDIIARIRHHGMIA
                     GLISNGYYFQPEKIRKLNEAGLDYLQISIDNVNPDDVSKKSLKVLDGKLKNLSEHARF
                     KVNINSVLGSGVANPDDALTIARRAVALGFSSTVGVLHDEMGLLKGLSEHEKDVYKKV
                     KALGTRSYARWNWFQDNLAEGEEYQWKCRAGARYLYIDEAGFVHWCSQQRGTPGIPLM
                     DYTQEDINREYITEKWCAPTCTIQCVHQVGHLDAWRDPQISLNDYNNRKGGKGLKKET
                     VAQVLGAE"
     gene            complement(1265..1960)
                     /locus_tag="VGB68_13405"
     CDS             complement(1265..1960)
                     /locus_tag="VGB68_13405"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HEY0050285.1"
                     /translation="MKKYFRSGFAAIAFALVFNVFAVTETKAQGGVNEILKRMDDHYK
                     VLSSLKADIIREKTNTQINETDRYQGNIVLLPGKGTKFSVRLNWTKPKEEILSVVNGQ
                     YVAYIPGQRRAYTGKSSSKTANEKGGNILKVMSMSKEELKANYDIVYLGKETVAGNIP
                     TWRLKLTPKKAASFKFAELWVDGNGMPVQGKVIALNNDTDTILLQNPVKNDTINGSIF
                     KIDLPKGTDVIKG"
     gene            complement(2090..2392)
                     /locus_tag="VGB68_13410"
     CDS             complement(2090..2392)
                     /locus_tag="VGB68_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050286.1"
                     /translation="MSFAKDIIQWNYGRGTWQYDVFCLLIIAFIFLTPKIWFDKRERL
                     ATQTARLIVKKEDFSPEKGALEKKVRELSGNENAEIIEWHERRNEQGEIFYEIDIR"
     gene            complement(2415..3101)
                     /locus_tag="VGB68_13415"
     CDS             complement(2415..3101)
                     /locus_tag="VGB68_13415"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0050287.1"
                     /translation="MKTILQTEDLTKNYKIGKIEVPALRGVSLAVEEGEFVAIMGPSG
                     CGKSTLLHLLGGLLSPSGGKILIDGEDLSQVSDAQRTDIRRRKIGFVFQRFNLFPTLT
                     ADGNLRLAERIHSGNGNGSAERRREVLRLLKLENKMHHKPLELSGGEQQRVALARAVV
                     NNPAIILADEPTGNLDTENSSIVLEMFRELNGRFNQTIIMITHNPEAAAGCHRIIRML
                     DGKIVDGERR"
     gene            complement(3258..4229)
                     /locus_tag="VGB68_13420"
     CDS             complement(3258..4229)
                     /locus_tag="VGB68_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050288.1"
                     /translation="MLKGENAAQEQLLAEVDRFARVNSMRAKMDLKFEDNSFAEFGVA
                     EKYKTADGEVVVQRPGKILLKVQIPIIKTDVAQMTSDGEKFRVAILEDGGTGKYKKFV
                     LGTNSADYTALQKEVDKIENGEAQALKQNVNAFANMRPQHFTEAMLLRPVDTAANVYV
                     QSEIFQEEFDINAKQKSPLRWVLRGYYLLDEISKQADGTMKISRRFWFDRVGGIRLAR
                     QQIFDASGDVESDIVYGREGDLTENGDYKKLPLRIEVTRPKEKYKMSLTYQDPTGVSI
                     GKTYPQAAFLLENLWNLPEVNLDEKLREMKTKQTQAEVEKSPTPNNK"
     gene            complement(4551..4907)
                     /locus_tag="VGB68_13425"
     CDS             complement(4551..4907)
                     /locus_tag="VGB68_13425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEY0050289.1"
                     /translation="MAEKHKTAGNAENTEHHHDEPHTVPLIYYFAVFAVLVVGTIITY
                     YVATIDLDSIFPGANTLVALLIAFFKMACVVLIFMHVRWMPRLIWLTALAGFFWLAIM
                     FAFTMQDYLTRSVLGR"
     gene            complement(4935..>5840)
                     /locus_tag="VGB68_13430"
     CDS             complement(4935..>5840)
                     /locus_tag="VGB68_13430"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HEY0050290.1"
                     /translation="SHDHHYHPPGLQHQFEDMGQQQESASIGMWMFLVQEIMFFGGLF
                     TVYLVFRSKYPIAFAAASNHLDAFWGGLNTLVLIVSSLTMALGVYYAQKSNRNMQVLM
                     IAVTMIFGTVFLGVKAIEYTDKYNHGLVPVDGWNKRMAGEAESVGANRVEPKSEPKTS
                     LDTPNSANEGHGNPRGEFKIDKDNDLKLVVQERAHLTESEKLGYFNNAGEIDADKFRD
                     KMRIFFWIYFTMTGLHALHMIIGLGIMAWLLWKAWRGSFTAEYYAPVEISGLYWHFVD
                     IVWIFLFPLLYLLGRHFMHGGGGGH"
BASE COUNT         1339 a         1540 c         1371 g         1590 t
ORIGIN      
        1 tttattttta ttcatactac aaagctccag cctttatcgt ttaaaccgcg gcgatattta
       61 ctttagactg aaagctttga caagattttt attcagcgcc gagaacctgt gcgaccgttt
      121 cctttttcaa gcctttgccg ccctttcggt tgttgtaatc gttgagcgaa atctgcgggt
      181 cgcgccacgc gtcgagatgc ccgacctggt gaacgcactg gatcgtgcag gtcggcgcgc
      241 accatttttc ggtgatgtat tcgcggttaa tgtcttcctg cgtgtaatcc atcagcggga
      301 tgcccggcgt tccgcgctgc tgcgagcacc agtggacgaa cccggcttcg tcgatgtaca
      361 ggtaacgcgc gccggcgcgg cacttccatt gatattcttc gccctcggcg agattgtcct
      421 gaaaccagtt ccagcgcgcg taggagcgcg tgccgagcgc cttcaccttt ttgtaaacgt
      481 ctttttcgtg ctcgctcaag cctttcagca agcccatctc gtcgtgcaga acgccgacgg
      541 tcgacgaaaa tccgagagcc acggcgcgcc gggcaatcgt cagagcatcg tccggatttg
      601 ccacgccgct gccgagaacc gagttgatat tgactttgaa cctggcgtgc tcggacaaat
      661 tttttaattt gccgtcgagc actttcaggc tctttttcga aacgtcgtcc gggttgacgt
      721 tgtcaatcga aatttgcaga taatccagac cggcttcgtt taatttcctg attttttccg
      781 gctggaaata atagccgttg gaaatcagac cggcaatcat tccgtgatga cggatgcggg
      841 cgataatgtc gtaaatttcg ggatgcagca tcggctcgcc gccggaaatc gtgatgacgg
      901 aagttccgaa ttcggcgagt ttgtcgatgc gcttgagcat cgtcgcaatc gccaccggct
      961 ctgaagtctt atcgtattcg ttgcaatagg tgcacgccag attgcaccgc cgcatcggga
     1021 cgatgtgcac caaaaccgga tgtttggtcg aagcaaaagc gcgaactgta tgacgaacac
     1081 cgcgcgtaaa gcgactgaaa ctgcttgcct tcggcatttt actctgattg tctcctgaaa
     1141 aactgctgga agcgtcaaat tgtaaattat agtctcattg ccgattatcg gcaaatcctg
     1201 tcggcgcgaa aggctccgcg cttttcagcc ggagcctttc gcgccgaata aaattcgctg
     1261 cccgttatcc cttgataacg tcggtgcctt tcggcaggtc gattttaaaa atcgagccgt
     1321 taatcgtatc gttctttacc ggattctgaa gcagaatcgt gtcggtgtcg ttgttcagcg
     1381 cgatgacttt tccctgaacc ggcataccgt ttccgtcaac ccacagctcg gcgaatttga
     1441 atgaagccgc ttttttcggc gtcagcttca agcgccaggt cggaatattg ccggcgaccg
     1501 tctccttgcc gagatacacg atgtcgtagt tagctttcag ctcttccttc gacatactca
     1561 tcactttcag tatgttcccg cctttttcat tggcggtttt cgagctcgac ttgcccgtgt
     1621 aggcgcgccg ctgaccggga atatacgcga cgtattgccc gttgacgact gaaagtattt
     1681 cttcttttgg cttcgtccaa ttaagacgaa ctgaaaattt tgttcctttt cccggcagta
     1741 aaacgatgtt tccctgatag cggtcggttt cgttgatctg cgtgttggtt ttttctctga
     1801 tgatgtcggc tttcagagat gaaagaactt tataatggtc gtccattcgt ttcaaaattt
     1861 cgttcacgcc gccctgcgct ttcgtttcgg tcacggcaaa gacgttgaaa acgagcgcga
     1921 aagcaatcgc cgcaaagcct gacctgaaat actttttcat ataataactc ctcgataact
     1981 ttgttttgct tttgtggaaa gtctctttga ttttaaattg cctgatttca aattccaaat
     2041 agcaattaat ccattaatca ggaatccgaa atttgtaaat tgaaattatt cagcgaatgt
     2101 caatctcgta gaaaatctcg ccctgctcgt tccggcgctc gtgccattcg atgatttcgg
     2161 cgttttcgtt tccgctcaat tccctgactt ttttctccag cgcgcctttt tccggcgaga
     2221 aatcttcttt tttgacgata agacgcgccg tttgggttgc aagcctttcc cgcttatcaa
     2281 accagatttt cggcgtcaga aaaataaaag cgatgattaa aagacaaaaa acgtcgtatt
     2341 gccacgtgcc gcgcccgtaa ttccattgga ttatgtcttt tgcaaaactc atctgtctct
     2401 gcttacggct tgccttagcg ccgctcgccg tctactattt ttccgtccag catacggata
     2461 atccggtggc agccggcggc ggcttccggg ttgtgcgtga tcataataat cgtctggtta
     2521 aaacgcccgt ttaattctct gaacatctcc agaacgatgc tcgaattttc cgtatcgaga
     2581 tttcccgtcg gctcgtccgc cagaataatc gccggattgt tgacgaccgc gcgcgccagc
     2641 gccacgcgct gctgctcgcc gccggaaagc tccagtggtt tatgatgcat cttgttttcc
     2701 agcttcaaaa gccgcaaaac ttcgcgccgg cgctcggcgc tgccgttgcc gttgccggaa
     2761 tgaattcttt ccgccagacg aagattgccg tccgccgtca aagtcgggaa tagattaaaa
     2821 cgctgaaaca caaaaccgat tttgcggcgg cgaatgtcgg ttcgctgcgc gtccgaaact
     2881 tgcgataaat cctcgccgtc aatcaatatt tttccgccgc tcggcgaaag cagtccgccg
     2941 agcagatgca gaagcgtcga ttttccgcag ccggaaggtc ccataatcgc cacaaattcg
     3001 ccttcttcga ccgccaggga aacgccgcgc agcgccggaa cttcaatctt cccgattttg
     3061 taatttttgg ttaaatcttc cgtctgtaga attgtcttca tcaaagcaaa tttttataaa
     3121 aacacagatt aacacagagt tttacaagta aaaagcaagc aaggcaaaag cgataaggca
     3181 aaaggcaaaa ggtagatttc ggaaatcctt taaccttttt tacttttgcc ttttgccttg
     3241 cttgcttttg ctatatctta tttgttattg ggcgtcgggc ttttttcgac ttccgcctgc
     3301 gtctgctttg ttttcatctc tcgcagtttt tcgtccaaat tgacttcggg cagattccag
     3361 aggttttcca gcagaaacgc cgcctgcgga taagttttgc cgatggaaac tccggtcggg
     3421 tcctgatagg tcaggctcat tttatatttc tctttcgggc gcgtgacttc aattctgagc
     3481 ggcagttttt tgtaatcgcc gttctcggtc aaatcgcctt ctctgccgta aacgatgtcg
     3541 gattcgacat cgcccgaagc gtcgaaaatc tgctggcgcg ccaggcggat gccgcccacg
     3601 cggtcaaacc agaagcggcg cgagattttc atcgtgccgt cagcctgttt gctgatttcg
     3661 tccagcagat aatagccgcg cagaacccag cgcaaaggcg atttctgttt ggcgttgatg
     3721 tcgaattctt cctgaaaaat ctcgctctgc acgtaaacgt tcgccgcggt atcgacgggg
     3781 cgaagcagca tcgcctcggt aaaatgctgc gggcgcatat tggcgaaagc gttgacgttc
     3841 tgcttgagag cctgtgcctc gccgttttcg attttgtcca cttctttctg caaagcggtg
     3901 taatcggcgc tgttcgtccc gagaacgaat tttttatatt tgccggttcc gccgtcttcc
     3961 agaatcgcca cgcggaattt ttcgccgtcc gaggtcatct gcgcgacgtc cgttttgata
     4021 atcggaatct gcactttcag cagaattttg cccgggcgct gaacgaccac ttcgccgtcc
     4081 gccgttttgt atttttccgc cacgccgaat tcggcaaacg aattatcctc aaacttcaaa
     4141 tccattttcg cccgcatcga gttaacgcgc gcgaagcggt ctacttcggc gagcaattgt
     4201 tcttgcgcgg cattctcgcc ttttaacaat ttcggtctgt ctattttttc cttgggcgag
     4261 atgcaaccgc ttaaaaatac agccgataaa aatattaaag ttaaaaataa tggcaatcgg
     4321 gcgctttgtt tcttccacat cagtcggttg tgatgtctga aataaagtgc ttgttgtctt
     4381 aaaaattctt ttaccacagt ttccataaaa gcaaaataaa accttattaa accgaaaata
     4441 atagcacgcc gccgtggttt gtcctaacaa acaaagacgc gaagccgaga agcattttgc
     4501 ttttcaaccc cgcgttcgta tcaatttttg ttcttatttc cttttaaata ttacctgccc
     4561 agaaccgagc gcgtcaggta atcctgcatc gtaaaggcaa acataatcgc cagccagaaa
     4621 aatccggcga gcgccgtcag ccagatgaga cgcggcatcc agcgcacgtg cataaaaatc
     4681 agcaccacgc aagccatctt gaaaaaagca atcagcaggg cgaccagcgt attcgcgccc
     4741 ggaaaaatgc tgtccaaatc aatcgtcgcc acataatagg taattatggt gccgacgacc
     4801 aaaaccgcga aaaccgcgaa ataatatata agcggaaccg tgtgcggttc atcgtggtga
     4861 tgttcggtat tttctgcgtt tcccgcagtt ttatgttttt ctgccataat aatatcccgt
     4921 tcaaatcctg aattttagtg tccgcctccg ccgccgtgca taaagtgtct gccgagcagg
     4981 tacagcagcg ggaaaagaaa aatccagacg atatcgacaa agtgccagta aagaccggaa
     5041 atttccaccg gcgcgtaata ttcggcggta aagctgccgc gccaggcttt ccacaaaagc
     5101 cacgccatga tgcccagccc gataatcata tgcagcgcgt gcaagccggt catcgtaaaa
     5161 taaatccaga agaaaatgcg catcttgtcg cggaatttat cggcgtcaat ctcgcccgcg
     5221 ttgttaaaat agccgagttt ttccgattcc gtcagatgcg ctctttcctg aaccaccagt
     5281 ttcaggtcgt tgtctttatc aatcttaaat tcgccgcgcg gattgccgtg tccctcgttt
     5341 gccgaattcg gcgtgtcgag agaggttttc ggctcgcttt tcggctcgac gcgattagcg
     5401 ccgacgcttt ccgcctcgcc cgccatgcgt ttgttccagc cgtcgaccgg aaccagcccg
     5461 tgattatatt tatcggtata ttcaatggct ttgacgccca gaaacaccgt gccgaaaatc
     5521 atggtgacgg caatcatcaa aacctgcata ttacgattgc ttttctgcgc gtaataaacg
     5581 cccagcgcca tcgtcaggct gctgacaatc aaaaccagcg tattcagacc gccccagaaa
     5641 gcgtccaggt gattgctcgc cgccgcaaac gctatcgggt atttggaacg aaacacgaga
     5701 taaaccgtga aaagcccgcc gaaaaacata atttcctgga cgagaaacat ccacattccg
     5761 atggaagcgg attcctgctg ctgtcccatg tcttcgaact ggtgctgcag tccgggcgga
     5821 tgatagtgat gatcgtgtga
//
LOCUS       DASWNE010000688         7975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_620006, whole genome shotgun sequence.
ACCESSION   DASWNE010000688 DASWNE010000000
VERSION     DASWNE010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7975
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620006"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2658)
                     /gene="ppdK"
                     /locus_tag="VGB68_13435"
     CDS             complement(<1..2658)
                     /gene="ppdK"
                     /locus_tag="VGB68_13435"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEY0050291.1"
                     /translation="MQPVSTEQEKMWSYLFEDCEAANTRDGKLLLGGKGAALAEMTGF
                     DLPVPPGFVITTDCCKKYHLNEKIFPDGMWRQAREKLTVIEEKLGKKFGDTENPLLVS
                     VRSGSPVSMPGMMDTVLNLGLNDKTVEGLARQTGDERFAWDAYRRFISMFSEIVLDVE
                     HEKFERVLERYKTGGRLDSDLTIEELKEIVAAYKRIIFTEESGFIFPENAESQLKMAI
                     AAVFDSWMGKRAVDYRRVNRISEDLGTAVTVQAMVFGNMGETSGTGVCFTRNPSTGEK
                     LLYGEYLLNAQGEDVVAGTRTPNPISRLQEELPEVFRQLSEITSSLEKHYRDMQDIEF
                     TIERGRLWMLQTRSGKRTGAAAVKVAVAMFKENLIDRKTALRRVSPEQVDQLLHPTID
                     PSAKVTVLATGLPASPGAAQGVAVFSPDEAEEMAKQGRKVILVRRETSPDDFHGMVAA
                     EAILTQRGGMTSHAAVVARGMGKPCVAGATVLNINYDHNEFSVGDETIPKGEWITVDG
                     STGRVFRGQIPTVQPELDEDFDLLMSWADETRRLKVRANSDTGHDSAVARNYGAEGIG
                     LCRTEHMFFGEDRLSVMREMILAGGLGEREKALAKLLPFQKQDFCEIFRAMDGLPVTI
                     RLLDPPLHEFLPSHTELMAELTELKYKARQVSPKEMDLLLDEIAEKRKLLRRVEHLSE
                     ANPMLGLRGCRLGLLVPEVTRMQVRAIFEAACEVQGEGATVLPEVMIPLVSTPEEFRD
                     QAALIREIGRKVMNEYGCELKYLVGTMIELPRAALVADKIAADAEFFSFGTNDLTQTT
                     YGLSRDDSGRFLPLYVEKKIYPDDPFQTLDTEGVGQLIKIAVERGRAVNPKLKIGVCG
                     EHGGDPRSIEFFNSLGLDYVSCSPFRVPIA"
     gene            3036..3800
                     /locus_tag="VGB68_13440"
     CDS             3036..3800
                     /locus_tag="VGB68_13440"
                     /inference="COORDINATES: protein motif:HMM:NF015060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HEY0050292.1"
                     /translation="MNGKPNRTEKIRQILVVLATAGVIIVNYLSGTGYINNATVGDVA
                     DKYPTLVTPAGYAFSIWGLIYLGMILFGVYQALPSQSANPHFLRTRTIYIASCAANCA
                     WIYFWLHERILIATAVIFILLAALAFINLNLKGANSSAETWLVRVPFGIYFGWVTVAS
                     ILNAAIALVFAGVEASNTTATVWACALIAVAAILGVLVRVKLGIGVYAITVAWALTAI
                     AVKQSGRTLIVTFCAFGVIALLIAFFTPFLRLNESK"
     gene            3797..5353
                     /gene="aroE"
                     /locus_tag="VGB68_13445"
     CDS             3797..5353
                     /gene="aroE"
                     /locus_tag="VGB68_13445"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEY0050293.1"
                     /translation="MNNGKICVSICAKTADELIQQIKRAEELADVIEIRFDCLDETVK
                     PESLWSDLKKLRQNFDGNLLATFRPAEQGGKRKLKLTERREFWSRSAVDEFVDWGDFE
                     LDFSGDDWHAYFRATLNRIIWSFHDFKGVPHNLDDIFLRLAELTEYPRSPRKKSDVVK
                     IAVQADDITDTIAVWKLLEKAKSENKKIIPIAMGESGKWTRILGLAHGAFMTYAALHT
                     GSETAPGQISARDLLEVYRAGELNEETAVYGIVGANTSYSMSPYIHNAAFKFHDLNSV
                     FVPLQVKNPDAFFRRMVKPETREIELNFKGFAVTIPHKRDIIKHLDSIDEAAREIGAV
                     NTVKIEDGKLSGYNTDARGFIEPLQNAFGDLKNARVAVFGAGGASRAACCALKKAGAE
                     VTVFARNADKAEALKTDFNVQIEQLSTAKNYKDFDILVNATPLGTKGEPANETPAVAA
                     QIENVKLVYDLIYNPFETKFIKEAKKAFVPALGGLSMLVAQAAAQQKIWTSLDAPIKE
                     MSAAALQRLS"
     gene            complement(5357..6091)
                     /locus_tag="VGB68_13450"
     CDS             complement(5357..6091)
                     /locus_tag="VGB68_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050294.1"
                     /translation="MNKILITLFLLFNFAAVSSAQDFVVPAESDYPQLRKEASKCEDF
                     VPQKWELVSKASGDLNRDNAPDCVLVIKGTDAKFRNKNDGLGTAVYDTNPRILVILFK
                     NAGKNLYELAKQSNSFIVIPDSPTMTEPFQEATVKNGVLQLSFEQWSSAGSWAAAQMS
                     YKFRFQNGDFVLIGADKTESMRNTGEMETRSYNFLTQKVRIETGNFSSDKKGKVRWKA
                     FKLDKLKTFDTFKAPFEWEIEPDYYI"
     gene            6151..7170
                     /locus_tag="VGB68_13455"
     CDS             6151..7170
                     /locus_tag="VGB68_13455"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEY0050295.1"
                     /translation="MNERYSRQILFGEIGRQGQEKLLNARVLLVGCGALGASHAETLA
                     RAGVGFLRIVDRDFVEFTNLQRQTLYSESDAGERLPKAIAAQNRIREINSEIETEAIV
                     ADVNHSNIESLVKDCDLVLDGTDNFQIRYLINDACVKLNKIWIYGAAVSSYGATMTVI
                     PSETPCLRCIFEEMPGAGSAPTCDTAGVIQPIITSVSAIQTTEALKILTGTLDRLHKS
                     LIQIDVWQNDWRKIKLGEPDADCKTCGRRNFEFLDAESAEFSAVLCGRDAVQIQPPKP
                     TEIDLVVLKEKLKNLGDVKQNEYLLRLTIDDFELTVFRDARAIVRGTDDVTTARSLYA
                     RYIGS"
     gene            7175..7684
                     /locus_tag="VGB68_13460"
     CDS             7175..7684
                     /locus_tag="VGB68_13460"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0050296.1"
                     /translation="MNTVLETERLVLRQWTLDDAPELFEICRDAEVMRYIGTRKPYKA
                     LDEAEKFLHRVGEYQKTNGFSRWAVIKKTSGKIVGSCGFAYTHEMPEPELGYLFERAV
                     WGKGFATEAARAVLNYGFEKLGFREIIAITDLENTASQRVLEKIGFTRRSVEKIDGED
                     SLIYLTRRV"
     gene            7696..>7975
                     /locus_tag="VGB68_13465"
     CDS             7696..>7975
                     /locus_tag="VGB68_13465"
                     /inference="COORDINATES: protein motif:HMM:NF024959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY0050297.1"
                     /translation="MNKIYGLLLSAALFALNVSASEPAIWTVDTRAEILRGDARGVSV
                     TDTGAVTLAPKLDRVFDTTQSYVWSSAVDASGNVYLGTGNDGRIFKVDA"
BASE COUNT         1862 a         2065 c         2146 g         1902 t
ORIGIN      
        1 cgctatgggc acgcgaaacg gcgagcagct gacgtaatcc aagccgagcg agttgaaaaa
       61 ctcgatcgag cgcgggtcgc cgccgtgctc gccgcaaacg ccgattttca gtttcggatt
      121 gacggcgcgc ccgcgctcga cggcgatttt tattaattgc ccgacgcctt ccgtgtcgag
      181 cgtctgaaac gggtcgtccg ggtaaatttt cttttcgaca tacaaaggca gaaatctgcc
      241 cgaatcgtcg cggctcaagc cgtaggtcgt ctgcgtcaaa tcgttggtgc cgaacgagaa
      301 aaattccgcg tcagccgcga ttttgtcggc gaccagcgcg gcgcgcggca actcgatcat
      361 cgtaccgacc agatacttca gttcgcagcc gtattcgttc atcacctttc tgccgatttc
      421 gcgaatcagc gccgcctggt cacgaaattc ttcgggcgtg gaaacgagcg gaatcatcac
      481 ttcgggcagc acggtcgcgc cttcgccctg cacttcgcag gcggcttcaa aaatcgcgcg
      541 cacctgcatt ctggttactt ccggcacgag caaaccgagc cggcagccgc gcaagcccag
      601 catcggattg gcttccgata aatgctcgac gcggcgcaac aattttcgtt tttccgcgat
      661 ttcgtcgagc agcaaatcca tttccttcgg actgacctga cgcgccttat attttaattc
      721 ggtcagctcc gccatcaatt ccgtgtggct cggcagaaat tcgtgaagcg gcgggtcgag
      781 caggcgaatc gtgacgggca atccgtccat cgcgcgaaaa atctcgcaaa aatcctgttt
      841 ttgaaacggc agaagctttg ccagcgcctt ttcgcgctcg cccaagccgc ccgccagaat
      901 catctcgcgc atcacggata agcggtcttc gccgaaaaac atatgctcgg tgcggcacag
      961 accgatgcct tccgcgccgt aatttcgggc gaccgccgaa tcgtgtccgg tgtcggaatt
     1021 ggcgcggact tttaaacgcc gcgtctcgtc cgcccacgac atcaaaaggt cgaagtcttc
     1081 gtccagctcc ggctgcacgg tcggaatctg accgcgaaaa actctgcctg tcgagccgtc
     1141 aaccgtaatc cactcgcctt tcggaatcgt ttcgtcgccg acgctgaatt cattatggtc
     1201 gtaattaatg ttcaggacgg tcgcgcccgc aacgcagggc ttgcccatac cgcgcgcgac
     1261 gaccgccgcg tgcgacgtca ttccgccgcg ctgcgtcaga atcgcttccg ccgcgaccat
     1321 tccgtgaaaa tcgtcggggc tggtttcgcg cctcacgaga atgactttgc gaccttgttt
     1381 cgccatttct tcggcttcgt cgggcgaaaa aacggcgacg ccctgcgccg cgcccgggct
     1441 tgccggaagt cctgtggcta aaaccgtgac ttttgcgctc gggtcgatcg tcgggtgcag
     1501 gagctggtcg acctgttcgg gcgagacgcg ccggagcgct gttttccggt caatcaaatt
     1561 ttccttaaac atcgccacgg cgaccttgac cgccgccgcg ccggtgcgtt ttccgctgcg
     1621 ggtttgcagc atccagagcc gcccgcgctc gatggtaaat tcgatgtcct gcatatcgcg
     1681 atagtgtttt tcgaggctcg aagtgatttc cgaaagctgc cggaaaacct ccggcagctc
     1741 ttcctgcagg cggctgatcg ggttcggcgt gcgcgttccg gcgacgacgt cttcgccctg
     1801 cgcgttcaga agatattcgc cgtagagaag cttttcgccc gtcgaaggat tgcgcgtaaa
     1861 gcagacgccc gtgccgctcg tctcgcccat attgccgaaa accatcgcct gtaccgtaac
     1921 ggcggttccc aaatcttccg agatgcgatt gacgcgtcga taatcgaccg cgcgcttgcc
     1981 catccacgaa tcgaagacgg cggcgatcgc cattttcagc tgcgattcgg cgttttcggg
     2041 aaaaataaaa ccggattctt cggtgaaaat aatgcgttta taagcggcga cgatttcttt
     2101 taattcttct atcgtcaaat ccgaatcgag ccgcccgccg gttttataac gttccagcac
     2161 gcgctcgaat ttttcgtgtt cgacgtccag cacaatctcg ctgaacatcg agatgaaacg
     2221 ccggtaagcg tcccacgcga atcgctcgtc gccggtctgc cgcgccaagc cttccacggt
     2281 tttatcgttt aagccgagat ttaaaaccgt gtccatcatt ccgggcatcg agacgggcga
     2341 gccgcttcgc accgacacga gcagcggatt ttccgtgtcg ccgaattttt taccgagctt
     2401 ttcttcgatt acggtcagtt tttcgcgcgc ctgccgccac attccgtcgg gaaatatttt
     2461 ttcgttcagg tgatattttt tgcagcagtc ggtcgtgatg acgaagccgg gcggcacggg
     2521 caaatcaaaa ccggtcattt cggcgagcgc cgcgcctttt ccgccgagca aaagcttgcc
     2581 gtcgcgcgta ttcgccgcct cgcagtcttc aaaaagatag ctccacattt tttcctgttc
     2641 ggttgataca ggctgcatag acacatttcc tctctgtcga aaaagattga actgcttaat
     2701 aaaaaaatct aaagacgcgg gagttttaga tttattccgc cggacgcggg cggcaattcc
     2761 tgatttgtgt gaagtataat ggtttttatc gaaagaaact agaagtaaac tcaaagaaag
     2821 atgacgcttc ggattgagaa attgaaattc gacttcgcgc gctttgcttc cggagcggga
     2881 aataatttct cgataaagtt aatcgagcgc atttgattcg taacgaaaat ttcgcgcgcg
     2941 aaggcgtcct gagagcgcga aattttgttt gaacattatt atttgaaacc ggcggtgaag
     3001 ttatcggtcg gcgattgtta taatatttaa aaactatgaa cggcaaacca aatcggacgg
     3061 aaaaaatcag gcagattctg gttgtcttag cgaccgccgg cgtgattatc gtcaattatc
     3121 tatcgggaac cggttacatt aacaatgcga cggtcggcga cgtcgccgac aaatatccga
     3181 cgcttgtcac gcccgccggt tacgcatttt caatctgggg cttgatttat ctcggaatga
     3241 ttctgttcgg cgtttatcag gcgctgccgt cgcagagcgc gaatccgcac tttctcagaa
     3301 ccagaacgat ttatatcgcg agctgcgcgg caaactgcgc gtggatttat ttctggctgc
     3361 acgaacggat tttgattgcg acggcggtga tttttatact gctcgccgcg ctcgctttta
     3421 taaatttgaa tttaaaaggc gcaaattcaa gcgccgaaac gtggctcgtt cgcgttccgt
     3481 tcggcattta tttcggctgg gtcacggtcg cgtcgattct caacgcggcg attgcattgg
     3541 ttttcgccgg cgtcgaagcg tcgaatacga cggcgaccgt ttgggcttgc gctttaatcg
     3601 cggtcgccgc gattttaggc gtgctggttc gcgtgaagct cggcatcggc gtctacgcta
     3661 taacggtcgc gtgggctttg acggcgattg cggtcaaaca aagcggcagg actttaatcg
     3721 tgactttttg cgctttcggc gtcatcgccc tgctgattgc gttttttacg ccgtttctgc
     3781 ggcttaacga atcaaaatga acaacgggaa aatctgcgtt tcaatctgcg cgaaaacagc
     3841 cgacgaattg attcagcaga taaaacgcgc cgaagaattg gctgacgtta tcgaaatccg
     3901 ttttgattgt ctggatgaaa ccgtcaagcc ggaaagtctc tggagcgatt taaaaaaact
     3961 caggcaaaat ttcgacggaa atctgctggc gacttttcga cccgccgaac aaggcggaaa
     4021 gcgaaaactg aaattgacgg aaagacgtga attctggagt cgttcagccg tcgatgagtt
     4081 tgtcgattgg ggcgacttcg agctcgattt ttccggcgac gattggcacg cttattttag
     4141 agcgacgttg aaccgaatca tctggtcttt tcacgatttt aaaggcgttc cccataatct
     4201 cgacgatatt ttcctgcgtc ttgccgaact tacggaatat ccgcgctctc cgcgtaaaaa
     4261 atccgacgtc gtaaaaattg ccgttcaggc agatgacatc acggacacaa tcgccgtctg
     4321 gaagctgctg gaaaaagcaa aatcggaaaa caaaaaaatc attccgattg cgatgggcga
     4381 gagcggcaaa tggacgcgga ttctgggctt ggcgcacggc gcttttatga cttacgcggc
     4441 gcttcacacg ggaagcgaaa ccgcgcccgg acagatttcg gcgcgggatt tgctcgaagt
     4501 ttaccgcgcc ggggaattaa acgaagaaac cgcagtttac ggcatcgtcg gcgcgaacac
     4561 gagctactcg atgtcgccgt atattcacaa cgcagcgttt aagtttcacg atttgaactc
     4621 ggttttcgtg cctctgcagg tcaaaaatcc ggacgctttt tttcgccgga tggtgaaacc
     4681 ggaaacgcgc gaaatcgaat tgaatttcaa aggctttgcc gtcacgatcc cgcacaagcg
     4741 ggacatcatc aaacatctgg attcgattga cgaagcggcg cgggaaatcg gcgccgtcaa
     4801 caccgtcaaa atcgaagacg gaaagctttc gggctacaac accgacgcgc gcggctttat
     4861 cgagcctctg caaaacgctt tcggagattt aaaaaacgcg cgggtcgccg tcttcggcgc
     4921 gggcggcgcg agccgcgcgg cgtgctgcgc gctgaaaaaa gcgggcgctg aagttaccgt
     4981 ctttgcgcga aatgcagata aagccgaagc gttaaaaacg gattttaatg ttcaaatcga
     5041 acagctctcg acggcgaaaa actacaagga tttcgatatt ctggtcaacg cgacgccgct
     5101 cgggacaaaa ggcgaaccgg caaacgaaac gcccgccgtc gccgcgcaga tagaaaacgt
     5161 caagctcgtc tacgatttaa tttacaatcc gtttgagaca aaatttataa aagaagcgaa
     5221 aaaggctttc gtcccggcgc tcggcggctt gtcgatgctc gtcgcgcagg cagcggcgca
     5281 gcagaaaatc tggacttcgc tcgacgcgcc gataaaggaa atgagcgcgg cggctttgca
     5341 aagattaagt tagaaatcag atgtaataat caggctcgat ttcccattca aacggcgctt
     5401 taaaagtgtc gaaagttttt aatttgtcga gcttgaaagc tttccatctg actttgcctt
     5461 ttttatccga gctaaagttt ccggtctcga tgcgcacctt ttgggtgagg aaattgtagc
     5521 ttctggtttc catttcgccg gtgtttctca tcgattcggt tttgtccgcg ccaatcaaaa
     5581 cgaaatctcc gttctggaaa cgaaatttgt atgacatttg cgccgccgcc cagcttcccg
     5641 cgctcgacca ttgctcgaaa ctcaattgca gaacgccgtt ttttaccgtc gcttcctgaa
     5701 aaggctccgt catggtcggc gaatcgggaa tgacgataaa actgttgctt tgcttcgcta
     5761 attcataaag atttttcccg gcgtttttga ataaaataac cagtatccgc gggttggtgt
     5821 cgtaaacggc agttcccaaa ccgtcgtttt tattgcgaaa tttggcgtcg gttcctttga
     5881 tgaccaaaac gcaatcgggc gcattgtcgc ggttcaaatc gccggaagct ttactcacaa
     5941 gctcccattt ctgcgggacg aaatcctcgc atttgctcgc ttctttcctt aactgcggat
     6001 aatccgattc cgccggtacg acgaaatcct gcgccgagga aacggcggca aaattaaaga
     6061 gcaaaaatag agtgattaat attttattca taaacttgct aaaatcgttt ttataatatc
     6121 aaagtgcttt cggaataaca ctcatttttt atgaacgaac gctacagccg gcagatttta
     6181 ttcggcgaaa tcggcagaca aggacaggag aagcttttaa atgcgcgcgt attgctggtc
     6241 ggctgcggcg cgctcggcgc atcgcacgcg gaaacgctgg cgcgcgccgg cgtcggtttt
     6301 ttgcggattg tcgaccgcga tttcgtcgaa tttacgaatt tgcagcggca gactttatac
     6361 agcgaatcgg atgccgggga gcgcttgccg aaagcgattg cggctcaaaa cagaatcagg
     6421 gaaatcaatt cggaaatcga gaccgaagcg attgtcgccg acgtcaatca ttcaaacatc
     6481 gaaagccttg ttaaagattg cgatttggtg ctggacggga cggataattt tcagattcga
     6541 tacctgataa acgacgcgtg cgtgaagctg aacaaaatct ggatttacgg cgcggcggtt
     6601 tcttcttacg gcgcgacgat gaccgtgatt ccttcggaga cgccgtgtct gcgctgtatt
     6661 tttgaagaaa tgccgggcgc gggcagcgcg ccgacctgcg acacggcggg cgtgattcag
     6721 ccgattatta cgagcgtttc ggcgattcag acgaccgaag ccctgaaaat tctgaccgga
     6781 actctcgacc ggctgcataa atctttaatt caaatcgacg tctggcagaa cgattggcgc
     6841 aaaatcaagc tcggcgagcc ggacgcggac tgcaaaacgt gcgggcggag aaattttgaa
     6901 tttttagacg cggaaagcgc ggaattttcc gccgttttgt gcggtcgcga cgcggttcag
     6961 attcagccgc cgaagccgac cgagattgat ttggtcgttt taaaggagaa acttaaaaat
     7021 ctgggcgacg tgaaacaaaa cgaatatttg ctgcgtctaa cgattgatga tttcgagctg
     7081 accgttttcc gggacgcgcg cgctatcgtg cgcggaaccg acgatgtcac gacggcgcgc
     7141 tcgctttacg ccagatatat cggaagctga acgaatgaat acagttttag aaaccgaaag
     7201 actcgttttg cgccagtgga cgctcgacga tgcgccggag ttgttcgaaa tctgccgcga
     7261 cgcggaagtg atgcgatata tcggcacgcg caagccttat aaagcgcttg acgaagccga
     7321 aaaattcctg catcgggtcg gcgaatatca aaagacaaac ggtttctcgc gctgggcggt
     7381 gattaaaaaa acaagcggga aaatcgtcgg cagctgcggt ttcgcctaca cgcacgaaat
     7441 gccggaaccg gagctcggct atttgttcga gcgcgcggtg tggggaaaag gttttgcgac
     7501 cgaagcggcg cgagccgttc tgaattacgg tttcgagaaa ttaggatttc gtgaaataat
     7561 cgctattacc gatttggaaa ataccgcctc gcagagagtt ttggagaaaa tcggttttac
     7621 tcggagaagc gtcgagaaaa tcgacggcga agacagttta atctatttaa caagaagggt
     7681 ttaggaataa tttgaatgaa taaaatttac ggtttgctat tgtctgcggc tttgtttgcg
     7741 ctgaatgtga gcgcgagcga gccggcgatt tggacggtcg acacgcgggc ggaaatcctg
     7801 cgcggcgacg cgcgcggcgt ttccgtgacg gacacgggcg cggtgacgct cgcgccgaag
     7861 ctcgaccgcg ttttcgatac gacgcaatcg tatgtctggt cgagcgccgt cgacgcttcc
     7921 ggcaatgttt atctcggaac cggcaacgac ggcagaattt ttaaagtcga tgcga
//
LOCUS       DASWNE010000689        24783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_482133, whole genome shotgun sequence.
ACCESSION   DASWNE010000689 DASWNE010000000
VERSION     DASWNE010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 24783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24783
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482133"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VGB68_13470"
     CDS             <1..323
                     /locus_tag="VGB68_13470"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEY0050298.1"
                     /translation="TEKEIDELVISQADDDEAWEKPIRVNREKVFSLNFPQPSEIVSE
                     KEILSGKPVFRGTRVPVSALLENPEAGVSLDEFLENFPTVRREQAVQVLDYFKKALAQ
                     LKAA"
     gene            371..640
                     /locus_tag="VGB68_13475"
     CDS             371..640
                     /locus_tag="VGB68_13475"
                     /inference="COORDINATES: protein motif:HMM:NF021552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEY0050299.1"
                     /translation="MCRNIKPLYNFEPATSDEEIRAAAIQFVRKISGFNKPSAVNAAA
                     FETAIEEIAAAAGKLLDSLVTPAAPRSRAVESEKARRRNQLRFGG"
     gene            complement(653..1153)
                     /locus_tag="VGB68_13480"
     CDS             complement(653..1153)
                     /locus_tag="VGB68_13480"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0050300.1"
                     /translation="MFSEQDIKNPAKREAIELFNEAFEAQQAGDYTEAIRLYKKSIAA
                     FPTAEAYTFLGWTYSFQGQYDLAIAECLAAISVDVNFGNPYNDIGSYLIAKGNYYDCV
                     RWFKLAMSAARYEARAYPHFNLAAVYEKRGKYLEAAKHYGLALDEQPNYAQAYKALRK
                     LQEKLN"
     gene            complement(1244..3256)
                     /locus_tag="VGB68_13485"
     CDS             complement(1244..3256)
                     /locus_tag="VGB68_13485"
                     /inference="COORDINATES: protein motif:HMM:NF025068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEY0050301.1"
                     /translation="MTKSNSFFSKALAVLVCTLLFAGFSLAQSLQQDLNQSFKAFKTF
                     RLDNNRLSSPATEPNGASLSVPTDAKTYVLNLTANDLRSPKYSAEEMTASGAQRLDFA
                     GVKTFKGKVSGEANSAARLTIDGGKVEGYFLVDAQKFYIEPARKYSESAAEADLIVYR
                     EDDFLNPETFDCHSELDKKIESGTQMVGLQSAAANLQTLRVIELATDADFEYVQAFGG
                     SSQANAEILSILNMVEGVYERELNLTFDIVFQHSWTTPDPFTGVNGDAFLRSFQNYWN
                     ANFSTVARDAAHLFTAKPNFVQQGFAFIGEICKPVSATRPSYAYGFSGKVDWAPAKYS
                     ITAHEIGHNLGASHNDTPDCAATLMNTTINALAPPTFCQASRTEIGNYVAANGTCLST
                     RNTGGSARFDFDGDGKSDLSVFRPANGSWYIINSGSNSFTGFQFGRVGDRIVPADYDG
                     DGRADAAVYRAGTWYLNRSSAGFTGVQFGLPDDIPAPADFDGDGKTDVAVFRPSTGYW
                     FISHSSTGAFIAVQFGVARDVPMPADFDGDRKADITVWRPSNGIWYRINSSDNTFFGA
                     QFGQLNDRPLMGDFDGDGKYDLAVFRPSNGYWYAAYSGSGGLLAAPFGIATDVPAPAD
                     FDGDGKTDLAVYRDGMWHRVTTANNSYSGVQFGIVGDVPAPSYYNQ"
     gene            3410..3862
                     /locus_tag="VGB68_13490"
     CDS             3410..3862
                     /locus_tag="VGB68_13490"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY0050302.1"
                     /translation="MWKSLVSSVWKRLPPVFRLKAIRLTQKKFTVSAVAVVLNEEGKI
                     LLLDHFLRPGASWALPGGFIEAGEQPEAAIKREIREETTLRLENVRLMGVRTINSHVE
                     ILFRAEATGKPVPNSTEINAAGWFSIDEMPSETSGVQKRIISEVLNQK"
     gene            3942..4556
                     /locus_tag="VGB68_13495"
     CDS             3942..4556
                     /locus_tag="VGB68_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050303.1"
                     /translation="MINLIPSPESVMEILQKTGAYRKGHFVYPNGKHASHYFQMPLAF
                     RYYDNARILSVGLSRMFRMDKTVSSRLPKVSIISPSPGGIMVAFGVREALNADQIYWA
                     ELEDGRRQFRQYIAEGDVYPAIIVDDILRSGRAIQETIDLVKGLGTEVIGIGTIVRFE
                     DSAPDFDGIAVKSLLTYDVNFYENEDQWRKSRSASDSEEETVRF"
     gene            complement(4674..6038)
                     /locus_tag="VGB68_13500"
     CDS             complement(4674..6038)
                     /locus_tag="VGB68_13500"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY0050304.1"
                     /translation="MKTSVKKLSLDDLIFHRQGSFVVRLIYAVFGFALCLFFRRIELV
                     NAESLPEKSGLIFVSNHPNALIDPALVFIALPRRIAFLAKSTLFKIPVLGFLIRTVGA
                     LPLYRQQDAGADVSKNQETFGVARELLKTGGAIALFPEGVSHNSPKLLPLKTGAARIA
                     LGAVSVGRESGSLDLQIVPVGLFYTNKTTFRSEALLHFGEPFPVSPVEFDGDGQPPKE
                     AVRHLTAQIEKALRRVTLNAESEAELSRARVAVEVFTSTTAHEENLAERLALLQRFVA
                     EPFAASGENLEKRLVEYDKRLGEHGIESEFLALAQYSKWFIFRQALRRLWKLLIFSPL
                     ALAGVILHFPAYQICKFLAFLQTRKGDYDMASTVKVLASIVLMPLTWAVFAAILYLYL
                     GWQIALISIPFSFLCGYVALRTLEEITELSGWAKAILLFIRKREKFLRLLAERGKLFE
                     KVKR"
     gene            complement(6144..6758)
                     /gene="recR"
                     /locus_tag="VGB68_13505"
     CDS             complement(6144..6758)
                     /gene="recR"
                     /locus_tag="VGB68_13505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEY0050305.1"
                     /translation="MLEYSEPVAKLIDEFKRLPGVGHKSAQRLAFYILRRPEEDIERF
                     VEALQEVKQKITFCTVCNNLTDVNPCMYCASANRDRSIICVIEEPYNLVAVEKTRSFR
                     GLYHILHGSLSPIRGVGPDELMLANIFPRLSPENNDGVEVREVIIATNPTTEGEATAN
                     YIARLLKPLGVRVTRIAMGMPVGSDLEFVDEVTMDRALANRHEI"
     gene            complement(6835..7143)
                     /locus_tag="VGB68_13510"
     CDS             complement(6835..7143)
                     /locus_tag="VGB68_13510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEY0050306.1"
                     /translation="MKFPGGFDMNSMMKQAQKMQEQMGKEMKAMTVDASAGGGAVSVK
                     MRGDFELLELTISPDLVKDGDVEMIQDLTLAAVNEAKRKVEESLKGKLGGMLPPGLGF
                     "
     gene            7407..10541
                     /locus_tag="VGB68_13515"
     CDS             7407..10541
                     /locus_tag="VGB68_13515"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEY0050307.1"
                     /translation="MPYLTEPSLAPDRREIVFVSGGDIWSVPSGGGTAQMLVAHPATE
                     SRPLFSPDGKRLAFVSNRTGNGDIYVLNLETNDLSRLTFDDGSDNLDAWSRDGRWIYF
                     SSSSRDISGMSDIFRVSAAGGTPQQVSNDRYASEFYAAPSPDGNSVAFTARGIANGQW
                     WRKGRSHIDESEIWLKNGDGGYEQLTQRGAKQLWTMWSADGSRIFYVSDRSGAQNVWT
                     QALKGQARQLTNFTDGRVLWANISYDGKQIVFERNFKIWTLNADTGRASEIAINLRGL
                     PAGTLTERVNLSSQIREIALSPDGKKISVVARGEIFAASSKDGGEATRVTNTVAPESF
                     AAWSPDSRKLVYSSEREGRLQIFQYDFAAETETNLTKSGDDFSPVYSPDGKFIAFIRN
                     ARSLWIYDVNTKQERELCKLYNDAPPLAGKRTIAWSPDNKWLAFLTYAPETRSYTNVS
                     VVSASGGAAQPLSFIANSNSGSLSWSPDNSFILFDTNQRTEEGSLARIDLRLRTPKFR
                     EDQFRDLFRQENPQDRPRPSPSPSPLASPSPIASPTPEDTKSNEIVFEDIRRRLSLLR
                     PGVNVNGQIISPDGKTLLILASAENQFNLYTMPLDELATDQSAKQITSTANFKSDAQF
                     SPDSKEVYYIENGRVNVVTLERREVRPLALNLEMNVNFAQEKMEIFKQGWRFLRDNFY
                     DDKYHGADWNAVEKTYEPLVAGARTTDEMRRLMSLMVGELNASHLGVQGNPGFTATPI
                     GKLGLRFDRNEYETSGRLKITEIIALSPVDITRSAKVGDYLLSIDGVQADARTNLDEL
                     LENKVGKRVELEISANADGSNKREVIVKPITTGAEKNLLYRHWVESNRAYVERISGGK
                     LGYVHLPDMGSGSLAQLYVDLDVQNQGREGVVVDIRNNSGGFINPYVIDVLARRGYLN
                     MKERGLWTVPARTALGQRALERPTILLTNQHSLSDAEDFTEGYRALKLGKVVGEPTAG
                     WIIFTWNVNTFDGTTVRLPRQLVTGGDGKNMELNPRPVDVQVTRPIGESLSGKDSQLD
                     AAVRELLAQTGRQP"
     gene            10688..11560
                     /locus_tag="VGB68_13520"
     CDS             10688..11560
                     /locus_tag="VGB68_13520"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY0050308.1"
                     /translation="MQIDWRTNPNQSPYLFRQMTEWSGVKLHRARVLPGRIIEQKAPW
                     HELHITLGGQLITEKISATGKLVTTRAAMGNLCLTPAGQAVAAHWDKPLDNMGILLNP
                     DFVVQTAVENRFASNFEFTEIYKNKDPLIQHIGLTLLAETGSETPAGRLYADSLIQTL
                     ILHLLKNYSTANAAAAENLNGGISGYKLRRVAEFIDANLEQDLSLAEIAAVADLSQYH
                     FARAFRKTTGLTPQQYVMQRRIERAKELLAKDDLPLVEISLRSGFKNQSHFTALFRKF
                     TKLTPKLWREMKLA"
     gene            11761..12465
                     /locus_tag="VGB68_13525"
     CDS             11761..12465
                     /locus_tag="VGB68_13525"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0050309.1"
                     /translation="MRKLPFLFIIVFALVAGSAAQTVRQSFEEGTRAAQNAEYEKALE
                     NYRKAILYTESEKTGDELLARIHFNIGVCLFHLKQNIEAVAELNRSIVLSKRGYQKAF
                     YVLGMAQAKLKNWRKAESAFRAAVKLKKDDAEAWFDLGLVLLEEKDFRAAEKAFQNSI
                     RYKTIAAAEAHNNIGVIYVLRGDFISAEKEFETALLESKGALGVARNNLQFCKAYKQI
                     FGGDLSAKLEFGRNEE"
     gene            12568..13020
                     /locus_tag="VGB68_13530"
     CDS             12568..13020
                     /locus_tag="VGB68_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050310.1"
                     /translation="MDKQQSMMAILKLYELRRDEKMRAARAWYFTEFAPNSAMDIIAL
                     YRGGEQASAYFRMVTSFWDMAASFVLNEAIDEKTFLDSGTEHIFVYAKIQPFLAEIRQ
                     MFGEPDYLINLEQLMHKIPGIETKLESRKRLIKLWTKENREFSATETK"
     gene            13253..15478
                     /locus_tag="VGB68_13535"
     CDS             13253..15478
                     /locus_tag="VGB68_13535"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEY0050311.1"
                     /translation="MKKYVIKRAGEGSLPEKLTRYREELNAEQFRVVTAKPQAALVVA
                     GAGTGKTRTITYRVAYLIEQGVSPQRILLATFTNRAAREMLRRVETLTGSQNVHRVWG
                     GTFHRIANLILRRHAVSIGYDSNYSILDAEDARDFVNVCIDEAGIDTKARRFPKAEVI
                     QDIISYANNTDTPVEDVIIRRYPYFEMLAQQIKRVDYIFQDRKRARNVMDYDDLLLNW
                     KRLLIEKPEIADLYAEQFQHILVDEYQDTNKLQAEIIDLLAVKHRNVMVVGDDAQSIF
                     AWRGAEFTNIYEFPQRYPEAATYKLETNYRSTPEILGLANTSIACNRKQFPKMLQAVK
                     KSKDFKPALIPCSNVEQQSAFIASRILELRDEGTSLEEIAVMYRSHYHSLELQLELTR
                     RNIPYRIQSGVRFFEQAHIKDVVSYLRIIVNPRDELAWKRILKMIPSVGSATANKIYE
                     SIAYAENPLAIIKSEALALKGASPRPRKDGEPNIEIDERPDSFEMSENENDAAPARAR
                     ASALPPHLPVSPKIRGKAGWQNFVDLIEILVRDENRNNPAKQIELILTNGYEQHLQET
                     FENAEARLEDLRQLALYASRYDSTESFLSELALLSTERFKEPQNVIGEDVIQGGEEDE
                     LLTLTSVHQAKGLEWKAVFLIWAAEGKFPSPRSLKEIDSEEEERRLWYVALTRAQDEL
                     YLTYPLMIVDYNRQTVLQKPSRFIMEVPPALFEVWGLEEEAPPLDAPIAVEDKGLEFL
                     N"
     gene            15596..16129
                     /locus_tag="VGB68_13540"
     CDS             15596..16129
                     /locus_tag="VGB68_13540"
                     /inference="COORDINATES: protein motif:HMM:NF038331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tsi3 family protein"
                     /protein_id="HEY0050312.1"
                     /translation="MCGAAAAAAFSKVKAIRLILFRLILALAAGCSPSPDAPGATTPS
                     PEENAREYVHKNGLTIELPEKFSVEETSEGFIIEPAGGANRNVRYPIEAQVILKKDEI
                     PPSREYAEQKTIGNRTIKYRINQNEGGSGGAEYNLTANEQIGDSVIYYSQSEQSEDVA
                     PHFKLCWTIIENTSLKK"
     gene            16332..17810
                     /locus_tag="VGB68_13545"
     CDS             16332..17810
                     /locus_tag="VGB68_13545"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0050313.1"
                     /translation="MMKFSLLKIRGIAAFALLVALLVTAAPVVLAQYDCDVPIPAEVR
                     TGAIAAGDPTQTSRVFRDGIPSSCTGGVPTAAPVAGSYRFDQYTFTNPTGLDACVKVF
                     LDASGCGGTANNSTQINAYSTFNPAMVMSGLIGKPGFSTIGTGSLNFPVAAGGSFTVV
                     AHEVVANGGCASYTLRVTYNTACRQAGFDRSSDNRADPTVFRPSNGTWYTTSTVSGEN
                     FIQFGSTNDIPAAGDYTGSNATDYGVYRPSNSTWYYGRNQTSPSTNFETIQFGASGDV
                     PAPGDYDGDGKQDVAVWRPSNGTWYVLRSGTTTLQVQQWGQNGDTPVWGDYDGDLQSD
                     FALVRNSGSNRLWLVLQSNFGYGFVLGCPTTAPICSNGVQWGLTTDKLVPGDYDGDAR
                     TDVAVWRPTDGNWYWIRSASKTVGNTPGATTSNTQWGANGDIPQPADYDGDKTMDFAV
                     FRSSPDPAQNFWYIRNSTGGTLTAIEWGQQGDQPATSPYRIQ"
     gene            17990..19285
                     /locus_tag="VGB68_13550"
     CDS             17990..19285
                     /locus_tag="VGB68_13550"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEY0050314.1"
                     /translation="MKKNGFLDSIRIASPCSQDWDEMSGNETVRFCEHCAFEVKNLSA
                     MKRREALRLVRQSEGRICVRYVKNPQTDAPVFAGKLYQITRRAGVAAGVLGASLTLST
                     LAYAQGEAVNPLSKPTIEASGKNQSDRDKTKSPTGTISGTTTDSTGAIIPNVTVKLSN
                     AESGESRNTTTDENGLYEFKDVLTGIYKVTAAATAGFAAYESQAIEVSENANIKLELS
                     MPLSLPRGASFTMGLVALARLEYEQPLLKAVSGENFEEVKALVAKGANVNAKDKNYGE
                     ATALHVAVENDNREIAKFLLDMGAKINARDKQRRTPLMSIDDDTKPELVRMLLDHRAK
                     VNALDAEGNTALIFAAQYENAEVLRVLIDAGARINAQNKEGKTALMLAAENDYYTSVE
                     VLLAAGADVHLRDKDGKTALDFADDEKIRDLLKSYNAKE"
     gene            complement(19392..20876)
                     /gene="crtI"
                     /locus_tag="VGB68_13555"
     CDS             complement(19392..20876)
                     /gene="crtI"
                     /locus_tag="VGB68_13555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HEY0050315.1"
                     /translation="MSKNAIIIGAGLGGLCAAIRLAKKGFAVTVLEKNAAVGGKVNIV
                     EASGYKFDTGASLLTMRHVLEELFVFAGKRVEDYLDIVPVEPICRYFWSDGTRFDASQ
                     NLAKTESEILRTTSRGEVENFRKFLADARRKYEVSEKTFLAHSLNDLPKLLRPKYLRD
                     LLAISSMRTLDAHVKNYFASPKLRQLFNRFATYNGSSPFQAPATFALIPYVEFGLGAW
                     YAKGGMYEIPRALGKLADELNVKIKTNSEVEKILVRNKKAIGVRLKNGERLESDFVIA
                     NSDAVETYRNLIESENTRIENLEPSSSGFVLLLGTKKRFPQLAHHNIFFSDDYFAEFE
                     AIFKDLRPARNPTVYVCAATRTDRTQAPEGCENLFVLINAPHTGAQTNWEKEKRDYRD
                     LIVGKLEDSGLKDLENSIDFEQIITPEDFEKKYRANRGSIYGVSSNGIFSAFLRPPNK
                     ARKIENLYFVGGATHPGGGIPLVLISGKLTADLILRQIQTGWTG"
     gene            21047..22039
                     /locus_tag="VGB68_13560"
     CDS             21047..22039
                     /locus_tag="VGB68_13560"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY0050316.1"
                     /translation="MKFCPTCQTRYDEEILRFCTKDGTPLVDENQPNFTAMPSESSID
                     DDDPGEQTVIRRNSPNVPPPVPDFAAGRTVQERIVVPTSEPVRAQTSPPPNVPPRKSS
                     TTAVVLLTLLGTLAVLGIGFGGWYLLQNRDADDANRNINVNTNPVNQNTNVNADNSPA
                     NFDFNINTNTNVNANVNTNVNANLKTPTPTPTRTPTPTPTRTPTPEANVNVNATVVNA
                     APASPTPTPTPRTTPTPSPQNVNVGMLNSRAVSLPKPAYPPTARQMNASGRVTVQVTI
                     DEEGNVLSAKAINGHVLLRNPAEAAARQSKFNPVRVGGQAVRASGVILYNFINQ"
     gene            complement(22138..23469)
                     /locus_tag="VGB68_13565"
     CDS             complement(22138..23469)
                     /locus_tag="VGB68_13565"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY0050317.1"
                     /translation="MTTDIAAGASACPVCSREAKDEYVPLVALEEDLQNLIRANAPDT
                     EEFQAVCARCVRLFERGKEQIIKDAAMQKDGSHVLSTPLRLDADERYTGGGITIAFLD
                     SGFFPHIDLTAPSNRILAYHNLSATGDGDASLFQSDVASWHGMMTSVVAAGNGSLSNG
                     FYRGIAPEANVVLIKLARTGRITEQNILDGLEWVLSNREKYGIRVVNISAGGDFEASY
                     LHDSLSQTVEECVREGITVVCAVGNAGHLATHPVFPPASAPSCIAVGGLDDNNSIKRS
                     KRGMYRSSYGPTVDGLQKPEIIAPSIWIPAPILPNTPTAQQAALLEQLDKAKDEELHK
                     IIEINPGIDGELDAALDRPPYMIRQIIALKIRQENVITRHYKYVDGTSFAAPIVSSVV
                     AQMLQANPNLTPQQIKRILISTAERLPHYEVDRQGWGVLDPRRAVEMAEKQ"
     gene            23714..24562
                     /locus_tag="VGB68_13570"
     CDS             23714..24562
                     /locus_tag="VGB68_13570"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY0050318.1"
                     /translation="MGRKKIGLALSGGAARGYAHLGVLKVLAEHDIPIDCIAGTSAGS
                     FAGAAFASGLSVDEIIAFSKKISWFNMTGFSYSPKGILSNAPMGNFIKQNFPFKTFEE
                     LPIPFAAVACDLETGEEVILKEGDLPFAVRASCAIPGVFVPLEDADSRKLIDGGVVAP
                     VPTKAVRKLGAEIVIAVDVISSGATYWGNPSTLLGVFFQSALMLLRTASKAHHYRADV
                     VIVPKIAHLRPDEIGKMDEFIKAGEEAALEKIDEIKKLVFPEDNSAAEDTENAEKENL
                     TTDLHR"
BASE COUNT         6133 a         6518 c         6452 g         5680 t
ORIGIN      
        1 taaccgagaa ggaaattgac gaactcgtaa tctcgcaggc tgatgacgac gaggcttggg
       61 aaaagccgat tcgcgttaac cgggaaaaag ttttttcttt gaattttccg cagccttccg
      121 agattgtcag cgaaaaggag attttaagcg gcaagccggt ttttcgcgga acgcgagttc
      181 ccgtttcggc tttattggaa aatccggaag ccggcgtttc gctcgacgag tttctcgaaa
      241 attttccgac tgtaagacgc gagcaagccg ttcaggtttt agattatttc aaaaaagctc
      301 tggctcaact gaaagccgct taatttcaaa ttaaaaagcc gccgaaactg acggcttttc
      361 atttttaatt atgtgcagaa atatcaaacc gctctacaat ttcgagccgg cgacgagcga
      421 cgaagagatt cgcgcggcgg cgattcagtt tgtccgcaaa atcagcgggt ttaataagcc
      481 ttccgccgtc aatgccgcgg ctttcgaaac ggcgattgaa gaaatcgcgg cggctgccgg
      541 aaagctgctc gattcgctcg tcacgcccgc ggcgcctcgg agccgcgccg tcgaaagcga
      601 aaaggcgcgc cgacgaaatc aactcagatt cggcggttaa ttcaattttt ggttaattca
      661 atttttcctg caatttcctg agcgctttgt aagcctgcgc gtaattcggc tgctcgtcca
      721 gagccaagcc gtaatgcttg gcggcttcga ggtattttcc gcgtttttcg taaacggcgg
      781 cgagattaaa atgcgggtag gcgcgggctt cataacgcgc ggcgctcatc gccagtttaa
      841 accagcgcac gcagtcgtaa taatttcctt tcgcaatcag gtaactgccg atgtcgttat
      901 acgggttgcc gaaatttacg tcaaccgaaa tcgccgccag acattcggca atcgccaaat
      961 cgtattgccc ctgaaaactg tatgtccagc ctaaaaacgt gtaggcttcg gcggtcggga
     1021 aagcggcgat tgatttttta taaagtctta tcgcttccgt gtaatcgccg gcttgctgcg
     1081 cttcaaacgc ttcgttgaaa agctcgatgg cttcacgctt tgccggattt tttatatctt
     1141 gctcggaaaa catctcagaa aaatttcaac acaaaattaa aagaggcaca aggacgaatc
     1201 cttgtacctc aaccctgctt gcgtattcag ttgtagagaa aacttattga ttgtaatacg
     1261 acggcgcggg aacgtcgccg acaatgccga actggacgcc cgaataagaa ttattcgccg
     1321 tcgtcacgcg atgccacata ccgtcgcgat aaaccgccaa atcggttttg ccgtcgccgt
     1381 cgaaatccgc cggtgcgggc acgtcggtcg cgatgccgaa cggcgcggcg agcaatccgc
     1441 cgcttccgct gtaagcggcg taccagtaac cgtttgacgg gcggaaaacc gccaaatcat
     1501 atttgccgtc gccatcgaaa tcgcccatca gcggtcggtc gttcaattgt ccgaactgcg
     1561 cgccgaaaaa cgtattgtcg ctgctgttga tgcgatacca gatgccgttt gacggacgcc
     1621 agacggtaat gtcggctttc cggtcgccgt caaaatcggc gggcatcgga acgtcgcggg
     1681 cgacgccgaa ctggacggcg ataaacgctc ccgtgctgct gtgcgagata aaccagtagc
     1741 cggtcgaagg tctgaaaacg gcgacatccg tcttgccgtc gccgtcgaaa tcggcgggcg
     1801 cgggaatatc atccggcaaa ccgaactgga ctccggtaaa gcccgcgctc gaacgattca
     1861 gataccacgt gccggcgcgg taaaccgccg cgtccgcccg cccgtcgccg tcgtaatccg
     1921 ccggaactat ccggtcgccg actcgcccga attgaaatcc tgtaaacgaa ttgcttccgc
     1981 tgttgatgat ataccagctt ccgtttgccg gtcggaaaac gctcaaatcg gatttgccgt
     2041 cgccgtcaaa atcgaaacgc gccgagcctc ccgtattgcg cgtgcttaaa caggttccgt
     2101 ttgccgcgac gtaattgccg atttccgtcc gcgaagcctg acaaaaagtc ggcggcgcga
     2161 gcgcatttat ggtcgtgttc atcagcgtgg cggcgcaatc gggcgtgtcg ttgtggctgg
     2221 cgcctaaatt atgtccgatt tcgtgcgccg tgatgctgta tttcgccgga gcccaatcga
     2281 ctttgccgct gaaaccgtaa gcataactcg ggcgcgtcgc cgaaaccggc ttgcaaatct
     2341 cgccgataaa cgcgaagccc tgctggacaa aattcggttt ggcggtgaaa agatgcgcgg
     2401 cgtcgcgcgc gacggtcgag aaattcgcgt tccagtaatt ctgaaacgag cgcagaaaag
     2461 catcgccgtt gacgcccgta aaagggtcgg gcgtcgtcca gctgtgctgg aaaacaatgt
     2521 cgaaagttaa attcagctcg cgttcgtaaa cgccttcgac catattcaaa atgctcaaaa
     2581 tttccgcgtt cgcctgactt gagccgccga aagcctgcac gtattcgaaa tcggcgtcgg
     2641 tcgccagctc aatcacgcgc agcgtttgca aatttgctgc cgccgactgc agcccgacca
     2701 tctgcgttcc gctttcgatt tttttgtcca gctcggaatg acaatcaaaa gtttcgggat
     2761 tcaaaaaatc gtcttcgcgg taaacaatca aatccgcttc cgcggcagat tccgaatact
     2821 ttcgcgccgg ttcgatataa aatttctgcg cgtcgacaag aaaataacct tcgacctttc
     2881 cgccgtcgat cgtcaggcgc gccgccgaat tcgcctcgcc cgaaactttt cctttgaacg
     2941 ttttaacgcc ggcgaaatcg agcctttgag cgccgctcgc ggtcatttcc tcggcgctgt
     3001 atttcggcga gcgcaaatcg tttgccgtca aattcaaaac ataagttttg gcatcggtcg
     3061 gaacgctcag cgaagcgccg ttcggctcag tcgcgggcga cgaaagccga ttattgtcga
     3121 gccgaaacgt tttgaaagct ttgaacgatt gatttaaatc ctgctgcagg ctttgcgcga
     3181 gcgaaaaacc cgcaaataga agcgtgcaaa caagtacagc taaagctttt gaaaaaaagg
     3241 agttagattt tgtcattgaa tcctctggcg gaaacgtata tggtttgaat tgtagaatgg
     3301 agctcggcta tggatatttg ccgataataa ataaaacgtt tatcgccgtc tattttccag
     3361 aaatgctttg gaaaaagatg atattgttcg aaaaaagttt gcctttaaaa tgtggaaaag
     3421 tctagtcagc agtgtctgga aacgattgcc gcccgttttt cgtctgaaag ccatccgcct
     3481 gacgcagaaa aaatttaccg tttcggcggt cgccgtcgtt ctcaacgaag aaggaaaaat
     3541 tcttcttctc gaccatttcc tgcgtccggg cgcgagttgg gcgctgcccg gcggatttat
     3601 cgaagcgggc gagcagcccg aagcggcgat taaacgtgaa atccgcgaag aaacgacgct
     3661 ccggctggaa aacgtccggc tgatgggcgt cagaacgata aacagtcacg tcgaaattct
     3721 ttttcgcgcc gaagcgaccg gcaagcccgt gccgaacagc acggaaatca acgccgccgg
     3781 atggttttcg attgatgaaa tgcccagcga gacgagcggc gttcaaaagc gaattatcag
     3841 cgaagtattg aatcaaaaat aatctcccgc cggcgggcac ttgttacccg tccgtttatt
     3901 cggctaagat taattaattc caaatttcag aggagagagc aatgattaat ttgattcctt
     3961 cgccagaatc ggtcatggaa attttgcaga aaacgggcgc gtatcgcaag ggtcatttcg
     4021 tttatccgaa cggaaaacac gcttcgcatt attttcagat gcctttggcg tttcgctatt
     4081 acgacaacgc gcggattctt tcggtcggtt taagccgtat gtttcgaatg gataaaaccg
     4141 tttcgagccg cctgcccaaa gtttcgatta tcagtccgag cccgggcggc attatggtcg
     4201 ccttcggcgt gcgcgaggcg ctgaacgccg accagattta ctgggcggag cttgaggacg
     4261 gcagacgtca atttcgtcaa tacatcgccg agggcgacgt ttatccggca ataatcgtgg
     4321 atgatattct gcgttcgggg cgcgccattc aggaaaccat cgacctggtc aaaggcttgg
     4381 ggaccgaagt tataggcatc ggcacgattg tcaggtttga agattccgcg cccgatttcg
     4441 acggcatcgc ggtcaagagc cttttgacct atgacgtaaa cttttacgaa aacgaagacc
     4501 aatggcgcaa gtcgcgcagc gcgtccgatt cggaagaaga aaccgtcagg ttttaaagcg
     4561 caaaagtgaa aaagtgaaag ggtgaaaaag gttgccgtcg aaaaataacg gttgcttttt
     4621 cacctttcta ctttttcact ttttcacctt ttcacttttt cactctttca cttttacctt
     4681 ttcacttttt caaacaattt gccccgttcc gccagcagcc gcaaaaactt ttcgcgtttg
     4741 cgaataaaaa gcagaatcgc ttttgcccag ccgctcaatt ccgtgatttc ttccagcgtc
     4801 cgcaaggcga cgtagccgca caaaaacgaa aacggaatcg aaatcaaggc gatctgccag
     4861 ccgagatata aatacaaaat cgcggcgaaa accgcccacg tcagcggcat taaaacgatg
     4921 ctcgccagca ctttaacggt cgaagccata tcgtaatcgc ctttgcgcgt ttgcagaaag
     4981 gcgagaaact tgcaaatttg atatgcggga aaatgcagaa tcacgcccgc cagcgcgagc
     5041 ggcgagaaaa tcaaaagctt ccataaacgc cgcaaagcct gccggaaaat aaaccatttc
     5101 gagtattgcg cgagcgcgag aaactcggat tcgatgccgt gctcgcccaa tcttttatcg
     5161 tattcgacga gccgtttttc cagattttcg ccgctcgcgg caaacggctc ggcaacaaat
     5221 ctttgcagca acgcgagccg ttcggcaaga ttttcttcgt gcgcggtggt cgatgtaaaa
     5281 acctcgacgg cgactctcgc cctcgaaagc tcggcttccg attcggcgtt gagcgtcacg
     5341 cgccgcaggg ctttttcgat ttgcgccgtt aaatgcctga ccgcttcttt cggcggctgc
     5401 ccgtcgccgt cgaactcgac cggcgaaacc gggaaaggct caccgaaatg cagcagagcc
     5461 tcgctgcgaa acgtcgtttt gttcgtgtaa aaaagcccga ccggaacgat ttgcaaatcg
     5521 agcgaaccgg actcgcgccc aaccgaaaca gcgccgagcg cgattcgtgc cgcgccggtt
     5581 tttaaaggca gaagcttcgg cgaattatgc gagacgcctt cggggaaaag cgcaattgcg
     5641 ccgcccgttt tcaaaagctc gcgggcgacg ccgaaagttt cctgattttt ggaaacgtcc
     5701 gccccggcgt cctgctggcg atagagcggc agcgcgccga ccgtgcgaat cagaaaaccg
     5761 agcaccggga ttttgaaaag cgtcgatttg gcgagaaagg cgatgcggcg cggcagcgcg
     5821 ataaaaacca gcgccgggtc aatcaaagcg ttcgggtgat tgctgacaaa aatcaaaccg
     5881 cttttttccg gcaggctctc cgcattgaca agctcgatgc ggcggaaaaa caaacacaga
     5941 gcgaagccga aaaccgcgta aatcagacgc acgacgaatg agccctgacg atgaaaaatc
     6001 aaatcgtcaa ggctcagttt ttttacgctg gttttcataa tcgataactg ataattgata
     6061 actgataact ggtaatggaa tttccaatcg ggcgttttca actctcagtt atcagttatc
     6121 agttatcagt tatcagtttt taactaaatc tcgtggcggt ttgccagtgc tctgtccatc
     6181 gtcacttcat cgacaaattc caaatcgctt ccgaccggca ttcccatcgc gattcttgtg
     6241 acgcggacgc ccaaaggctt cagcagccgc gcgatgtaat ttgccgtcgc ctcgccttcg
     6301 gtcgtcggat tcgtggcgat aataacctcg cggacttcga cgccgtcgtt gttttcggga
     6361 ctcagacgcg gaaaaatatt tgccagcatc aattcatccg gaccgacgcc gcgaatcggc
     6421 gaaagacttc cgtgcagaat gtgatacaaa cctctgaagg agcgtgtttt ttcgaccgcg
     6481 accagattgt aaggctcttc gatcacgcag attatcgagc ggtcgcgatt ggctgaggcg
     6541 caatacatac acgggttgac gtcggtcagg ttgttgcaaa ccgtgcaaaa ggtaattttt
     6601 tgtttgactt cctgcaaagc ctcgacgaag cgctcgatgt cttcttcggg gcggcgcaaa
     6661 atataaaacg ccagacgctg cgcgcttttg tgcccgacgc cgggcagacg tttgaattcg
     6721 tcgattaact tggcgaccgg ttctgaatat tcaagcataa ataataataa gcggtaaacg
     6781 gtaaacggta agctcgctgc cttgaaattg cagcttaccg cttaccgctt accactagaa
     6841 accgagtccg ggcggcagca ttccgccgag ttttcctttc agcgattctt cgactttgcg
     6901 ttttgcctcg ttaacggcgg cgagcgtcaa atcctgaatc atttccacgt cgccgtcttt
     6961 taccaagtcg ggcgaaatcg tcaattccag cagctcaaaa tcgccgcgca tctttaccga
     7021 gaccgcgccg ccgcccgccg aagcgtcgac ggtcatcgcc ttcatttcct tgcccatctg
     7081 ctcctgcatt ttctgggctt gcttcatcat cgagttcatg tcaaaaccac cgggaaattt
     7141 cattgcatta ttttcctttt atgttttaat ttatacatta atcgtaaccg tccgaagaat
     7201 taaatgcaaa cctttaaatc cgcccgtttt cgattatttt ttgaattacc tgaacaaaac
     7261 aattatcatc agcgtaagca tttctgcttt ctaaatttgt cactgaaaaa taaaaaaata
     7321 tgttgcctgt aaaattttcc gctcgatttt ttttcctttt aagctgcctt tgtttaaacg
     7381 ccgccgcggt tttcgctcaa gcgccgatgc cttatctgac cgagccgtcg ctcgcgcccg
     7441 accgccggga aatcgttttt gtttccggcg gcgacatctg gtcggttcca tccggcggcg
     7501 ggacggcgca gatgctcgtg gcgcatccgg cgaccgagtc gcgcccgctg ttttcgcccg
     7561 acggaaagcg tctggcgttt gtctcgaacc gcacgggtaa cggcgacatt tacgttttaa
     7621 atctggaaac aaacgattta tcgcgtctga cttttgacga cggctcggac aatctcgacg
     7681 cctggtcgcg cgacgggcga tggatttatt tttcgtcttc gagccgcgat atttcgggaa
     7741 tgagcgacat tttccgcgtt tcggcagcgg gcggaacgcc gcagcaggtc tcgaatgacc
     7801 gctacgcgag cgagttttac gccgcgcctt cgccggacgg aaacagcgtc gcttttaccg
     7861 cgcgcggaat cgccaacggg caatggtggc gaaaaggcag aagccacatc gacgagagcg
     7921 aaatctggct gaaaaacggc gacggcggtt acgagcagtt gacgcagcgc ggcgcgaaac
     7981 agctctggac gatgtggagc gccgacggct cgcgtatttt ctacgtttca gaccgcagcg
     8041 gcgcgcagaa cgtctggacg caagccctga aagggcaagc ccgccaattg acgaatttca
     8101 ccgacggcag agttttgtgg gcgaatattt cctacgacgg aaagcaaatc gttttcgagc
     8161 gcaattttaa aatctggacg ctgaacgccg acacgggacg ggcgtccgaa atagcgataa
     8221 atctgcgcgg gcttcccgcc ggaacgctga ccgaaagagt caatctctcg tcgcaaatcc
     8281 gcgaaatcgc gctttcgccg gacggcaaaa aaatctcggt cgtcgcgcgc ggcgaaattt
     8341 ttgcggcttc ttcaaaagac ggcggcgaag cgacgcgcgt cacgaatacg gtcgcgcccg
     8401 aatcttttgc ggcgtggtcg cccgacagcc gaaagctggt ttattcttcg gagcgcgaag
     8461 ggagactcca gatttttcaa tacgatttcg ccgcggaaac cgaaactaat ttaacgaaaa
     8521 gcggcgacga tttttcgccc gtctattcgc cggacggaaa atttatcgcc tttatccgca
     8581 atgcccgctc gctctggatt tacgacgtga atacgaaaca ggaacgcgag ctttgcaaac
     8641 tttacaacga cgcgccgcct ttagccggca aacgcacaat cgcctggtcg ccggacaata
     8701 aatggctcgc gtttttgact tacgcgccgg aaactcgttc ttatacaaac gtctcggtcg
     8761 tttcggcaag cggcggcgcg gcgcaaccgc tcagctttat cgccaattca aacagcggct
     8821 cgctgtcgtg gagtcccgat aattccttta ttttgttcga cacgaaccag cgcacggaag
     8881 aaggctcgct ggctcgcatc gatttgcgcc tgcgcacgcc gaaatttcgc gaagaccagt
     8941 ttcgagacct tttccggcag gaaaatccgc aggacagacc gcgcccgtcg cccagcccaa
     9001 gcccgctggc ttcgccttcg ccgattgcct cgccgacgcc cgaagacacg aaatccaatg
     9061 agattgtttt tgaagatata cgccgccgcc tgagcctttt gcgtccgggc gtcaatgtta
     9121 acgggcagat tataagtccc gacggaaaga cgcttttgat tctcgcgtcc gccgaaaatc
     9181 agtttaatct ctacacgatg ccgctcgacg agctggcgac cgaccaatcg gcaaaacaaa
     9241 tcacttcgac ggcgaatttc aaatccgacg cgcagttttc gcccgattcg aaagaggttt
     9301 attacatcga aaacggtcgg gtaaacgtcg tcacgctcga gcgccgcgag gttcgcccgc
     9361 tcgcgctgaa tctggaaatg aacgtcaatt tcgcgcagga aaaaatggag attttcaaac
     9421 agggctggcg atttttgcgc gacaattttt acgacgacaa atatcacggc gcggactgga
     9481 acgcggtcga aaaaacttat gagccgctgg ttgcgggcgc gcggacgacc gacgaaatgc
     9541 ggcgtctgat gtcgctgatg gtcggcgagc tgaacgcctc gcatctcggc gttcagggca
     9601 atccgggctt tacggcaacg cccatcggca agctcggctt gcgtttcgac agaaatgaat
     9661 atgaaacgag cgggcgcttg aaaattacgg aaatcatcgc tttgagtccg gtcgatatta
     9721 cgcgcagcgc gaaagtcggc gattatttgc taagcatcga cggcgtgcag gcggacgcgc
     9781 gaacaaatct ggatgaattg ctggaaaaca aagtcggcaa acgcgtcgag ctggaaattt
     9841 ccgccaacgc ggacggctcg aacaaacgcg aggtgattgt caaaccaatc acgaccggcg
     9901 cggagaaaaa tctgctttat cgtcattggg tcgaatcgaa tcgcgcttat gtcgagcgca
     9961 tcagcggcgg caagctcggc tatgttcatt tgcccgatat gggttcgggc agtctggcgc
    10021 agctttacgt cgatttggac gtgcagaatc agggcagaga aggcgtcgtc gtcgatattc
    10081 gcaacaacag cggcggattt atcaacccgt atgtgattga cgttctcgcg cggcgcggtt
    10141 atctgaatat gaaggagcgc ggtctgtgga cggttccggc gcgcaccgcg ctcggacagc
    10201 gcgcgctgga acgcccgaca attttgctga ccaatcagca ttcgctttcc gatgccgaag
    10261 attttaccga aggctatcgc gctttaaagc tcggcaaagt cgtcggcgag ccgaccgccg
    10321 gctggattat ttttacgtgg aacgtcaaca ctttcgacgg cacgaccgtg cgcctgccgc
    10381 gccagctggt gacgggcggc gacggcaaaa atatggagct taatccgcgt cccgtcgatg
    10441 ttcaggtcac gcgaccgatc ggcgaatctc taagcggaaa agactcgcag ctcgacgccg
    10501 ccgtgcgcga gcttctggcg cagaccggaa ggcagcctta atgcgaaacg caaaacgcga
    10561 agtgcaaaat attaggattt aaaattcaag attccgaaat taaatttctt ccattttgca
    10621 ttttgcgttt tgcgtttcgc gtttttaacg cttgcttttt ccccggacct gaattaaata
    10681 ttattttgtg caaatcgatt ggagaacaaa tccgaatcaa tcgccgtatc tatttcgcca
    10741 gatgacggaa tggtcgggcg taaagcttca ccgggcgcgt gttttgccgg gcaggataat
    10801 tgaacaaaaa gcgccctggc acgagctcca cattacgctc ggcggtcaat tgattaccga
    10861 aaaaatttcg gcgaccggaa aactcgtaac gaccagagcg gcgatgggaa atctttgtct
    10921 gacgcctgcc ggacaggcgg tcgcggcaca ttgggataag ccgctggaca atatgggaat
    10981 tttgctcaat cccgattttg tcgttcagac ggcggtagaa aatcgctttg cctcgaattt
    11041 tgagtttacc gaaatttata aaaacaaaga ccctttgatt caacacatcg gcttgaccct
    11101 gctcgccgag accggctctg aaacgcccgc cggcaggctt tacgccgatt ctttaatcca
    11161 gactctgatt ctgcatctgc taaaaaatta cagcacggca aatgccgccg cagccgaaaa
    11221 tttgaacggc ggcatttccg gctataagct gcgccgcgtc gccgaattta tcgacgccaa
    11281 tctggaacag gatttgagcc tcgcggaaat cgccgccgtc gccgatttga gccagtatca
    11341 tttcgcgcgc gccttccgca aaacgaccgg gctgacgccg cagcaatacg tgatgcagcg
    11401 gcgcatcgaa cgcgccaaag agctgctggc aaaagacgat ttaccgctgg tcgaaatcag
    11461 cctgcgctcc ggctttaaaa atcaaagtca tttcaccgcg cttttccgca aattcaccaa
    11521 gctgacgccg aaactctggc gcgaaatgaa gctcgcataa aagtgaaaaa gtgaaaaagt
    11581 gaaaaagttt gccgtcaaaa ataacgatgg ctttttactc tttcactttt aacctttttc
    11641 actttttcaa ctttatcagc aatttcagaa tataaaccgc aagcgggcga aagattcccg
    11701 gcgcacgtat tttttaaact cttttcaaga cttggaaaaa gtctgaggag atttaagatt
    11761 atgcgtaaac taccgtttct ttttattatc gttttcgcgc tcgtcgccgg ttccgcggcg
    11821 caaaccgtca ggcaatcgtt tgaagaaggc acgcgggcgg cgcaaaatgc cgaatacgaa
    11881 aaggcgctcg aaaattaccg gaaggcgatt ctttacacgg aaagcgaaaa aaccggcgac
    11941 gagcttctgg cgcgaattca tttcaacatc ggcgtctgtc tttttcatct gaaacaaaac
    12001 attgaagccg tcgcggaact caatcggtca atcgttctga gcaaacgcgg ttatcaaaaa
    12061 gccttctatg tgctcggaat ggctcaggcg aagctgaaaa actggcgaaa agccgaaagc
    12121 gcgtttcgcg cggcagtcaa gctcaaaaaa gacgacgccg aagcgtggtt tgatttagga
    12181 ttggttttgc tggaagaaaa ggattttcga gccgctgaga aagcgtttca aaattcaatc
    12241 cgctataaaa caatcgccgc cgccgaggcg cacaataaca tcggcgtgat ttacgtgctc
    12301 agaggcgatt ttatctcggc ggaaaaagaa tttgagacgg ctttgctcga atcgaaaggc
    12361 gcgttgggcg tcgcgcgaaa caatctgcaa ttctgcaaag cgtacaaaca aattttcggc
    12421 ggagatttgt ccgcgaaact ggaattcggc agaaacgaag aatgaaggcg aaagcaagca
    12481 aggcaaatca ggatttaact tttacttttt aacttccgct tttttacttt tttgtttctt
    12541 ttccgcaaaa tttttcagga gtataaaatg gacaaacagc aatcaatgat ggcgattctg
    12601 aagctttacg aattgcgccg cgacgagaaa atgcgggcgg cgcgagcgtg gtatttcacg
    12661 gaattcgcgc cgaacagcgc gatggatatt atcgcgcttt atcgcggcgg cgagcaggcg
    12721 agcgcgtatt tccggatggt cacttcgttc tgggacatgg cggcgtcttt cgttttaaac
    12781 gaagcgatag acgaaaaaac gtttctcgat tccggcacgg aacatatttt cgtctacgcg
    12841 aaaatccagc cgtttttagc ggaaatccgc caaatgttcg gcgagcctga ttatttaata
    12901 aatttagagc aattaatgca taaaattccg ggcatcgaga ccaagctcga aagccgcaaa
    12961 cgattgatta aactctggac gaaagaaaac cgggagtttt cggcaacgga aaccaaataa
    13021 ttttttcaaa taattttcaa aagcctttgc cgatttaatc catcggcaga ggctttattt
    13081 gtttagcgga tggataacga agtaaaataa ccgacaaatt atgcgctcgc cggtcgaaaa
    13141 acccgaaaaa agacttttac tggaattcag cgatctgccg catctttacg gagcgttatt
    13201 tagagcgttg aagcggcgaa aaccgggcgg cgaatgataa aataaaacct cgatgaaaaa
    13261 atacgtcatc aaacgcgcgg gcgaaggctc gctgccggaa aagctgacgc gctatcgcga
    13321 ggaactgaac gccgagcagt ttcgcgtcgt cacggcgaag ccgcaagccg cgctggtcgt
    13381 ggcgggcgcg ggaacgggca aaaccagaac gataacttat cgcgtcgctt atttgattga
    13441 acagggcgtt tcgccgcaga gaattcttct ggcgactttt accaatcgcg ccgcgcgcga
    13501 aatgctccgg cgcgtcgaaa cgctgaccgg ctcgcaaaac gtgcatcgcg tctggggcgg
    13561 cacgtttcac cggattgcga atctgattct gcgccgtcac gccgtatcaa tcggctacga
    13621 ttcgaattat tcgattctcg acgccgaaga cgcgcgggat ttcgtcaacg tctgcatcga
    13681 cgaagccgga atcgacacga aagccaggcg ttttccgaaa gccgaagtca ttcaggacat
    13741 aatttcatac gccaacaaca ccgacacgcc cgtcgaagac gtaatcattc gccgctaccc
    13801 gtattttgaa atgctcgcgc agcagatcaa gcgcgtcgat tatatttttc aggaccggaa
    13861 acgcgcgcga aacgtgatgg attatgacga tttgctttta aactggaaac ggcttcttat
    13921 cgaaaagccc gaaatcgccg acctttacgc cgaacagttt caacacattc tggtcgacga
    13981 atatcaggac acgaacaagc tgcaagccga aattatcgac cttctggcgg tcaaacatcg
    14041 aaacgtgatg gtcgtcggcg acgacgcgca aagcattttc gcgtggcgcg gcgcggaatt
    14101 tacgaacatt tacgaatttc cgcagcgtta tcccgaagcc gcgacctaca aactggaaac
    14161 gaattatcga agcacgccgg aaattttagg tctggcgaac acttcgattg cctgcaatcg
    14221 aaagcagttt ccgaaaatgc tgcaggcggt aaaaaaatcg aaggatttca aacccgcgct
    14281 gattccgtgt tccaacgtcg agcagcaatc ggcgtttatc gcttcgcgga ttctggaact
    14341 gcgcgacgaa ggcacgtcgc tggaagaaat cgccgtgatg tatcgctcgc attatcattc
    14401 gctcgaactg caactggaat taaccagaag aaatattccg tatcgcatcc agtcgggcgt
    14461 cagatttttc gagcaggcgc atatcaagga cgtggtttcg tatctcagaa tcatcgtcaa
    14521 cccgcgcgac gagctggcgt ggaagcgaat tttgaaaatg attccgagcg tcggcagcgc
    14581 gacggcgaat aagatttacg aatcaatcgc gtatgcggaa aatccgctgg caataattaa
    14641 atcggaagcg ctggcattaa aaggcgccag cccgcgtccg cgcaaggacg gagaaccgaa
    14701 tatcgaaatt gacgaaagac ctgattcttt cgaaatgtcc gaaaacgaaa atgatgccgc
    14761 tcctgcccgg gcgcgggctt ccgctttgcc gccgcatttg ccggtttcgc cgaaaattcg
    14821 cggcaaagcg ggctggcaga attttgtcga tttaatcgag attctcgtgc gcgacgaaaa
    14881 ccgaaacaat ccagccaaac aaatcgagct gattctgacg aacggctacg agcagcattt
    14941 gcaggaaact ttcgaaaacg ccgaagcgcg gctcgaagat ttacgccagc tggcgcttta
    15001 cgccagccgt tacgattcga ccgaaagctt tttgtccgag ctggctttgc tttccaccga
    15061 gcgctttaaa gagccgcaaa acgttatcgg cgaagacgtt attcagggcg gcgaagaaga
    15121 cgagcttttg actttaactt cggtgcatca ggcgaaaggg ctggaatgga aagccgtttt
    15181 tctcatctgg gcggcggaag gcaaatttcc cagcccgcgc tcgctgaaag aaatcgacag
    15241 cgaggaagaa gagcggcggc tctggtatgt cgcgctgacg cgggcgcagg acgagcttta
    15301 tctgacgtat cctttaatga ttgtcgatta caaccggcag accgttctgc aaaagccttc
    15361 gcgttttata atggaggttc cgcccgcgct gttcgaggtt tggggtctgg aagaagaagc
    15421 gccgccgctg gacgcgccaa tcgccgtcga agataaaggt ctggagtttt taaattagtc
    15481 ttcaaaaact ctttggcgca attgccgtca aattcagatt tcggatgggc gtcaagacaa
    15541 tctgtttttc cgattccgac agtttaagaa atttcgtttt ggagacatat ttaagatgtg
    15601 cggagcagcg gcagcagcgg cgttttcaaa ggttaaagca atccgtttaa tactgttccg
    15661 gcttatttta gctttagcgg cgggctgctc gccgtcgccg gacgcgccgg gagcaacgac
    15721 gccttctccc gaagaaaatg cgcgcgaata cgttcacaaa aacggtttga caatcgagct
    15781 gcccgaaaaa ttttccgtcg aggaaacgag cgaaggtttt attatcgaac ctgccggcgg
    15841 cgcgaatcga aacgttcgtt atccaatcga agcgcaggtc attctgaaaa aagacgaaat
    15901 accgccgagc cgtgaatatg cagaacaaaa aaccatcgga aatcgcacta taaaataccg
    15961 gattaaccaa aacgaaggcg gctcaggcgg cgcggaatac aatcttacgg ctaacgagca
    16021 aatcggcgac tcagtcattt attacagcca aagcgaacaa agcgaagacg tcgcgccgca
    16081 ctttaaactc tgctggacaa tcatcgaaaa cacctcgctc aaaaaataat gccgccttta
    16141 acgcggaaac ccgggctttg ttcttaaacg atgaatgagg cgccaaataa ctattgtaaa
    16201 ttttcgtaaa gaggcgtatt ttttaattgt tacctataaa tgacgctttt cattcgtaaa
    16261 gaaatctcga cggtcttttt tgtaaaaatt ttcggagacg cgcgcgtctc cgctgctaag
    16321 gagatttaga aatgatgaag ttttcccttt tgaaaatcag gggcatagct gctttcgctt
    16381 tgctcgttgc gcttcttgtg actgccgcgc cggttgtgct ggcgcagtat gattgcgatg
    16441 ttcctattcc ggcggaggtg agaaccggcg cgattgcggc gggcgacccg acgcagacga
    16501 gccgcgtttt ccgcgacggc attccatcga gctgcacggg cggcgtgccg acggcggctc
    16561 cggttgccgg aagttaccgt tttgaccagt atacttttac caatccgacc gggctggacg
    16621 cttgcgtgaa agtttttctc gacgcgagcg gctgcggcgg aacggcaaac aattcgacgc
    16681 agattaacgc ttattcgacc tttaatccgg cgatggtgat gagcgggctg attggaaaac
    16741 ccggattcag caccatcggc acaggctcgc tcaactttcc ggtggcggcc ggcgggtcgt
    16801 ttacagtggt cgcgcacgaa gtcgtggcga acggcggctg cgcaagctac acgcttcgcg
    16861 taacttacaa cacggcttgc cgtcaggcgg gctttgaccg ttcgagcgac aatcgcgccg
    16921 acccgaccgt ttttcgaccg tcaaacggca cctggtatac gaccagcacg gtgagcggcg
    16981 agaattttat tcaattcggc agcaccaatg acattcccgc ggcgggcgat tatacgggca
    17041 gcaacgcgac cgattacggc gtttaccgtc cgagcaacag cacctggtat tacggcagaa
    17101 atcaaacttc gccgagcacg aatttcgaaa cgattcagtt cggcgcgagc ggcgacgttc
    17161 ccgcgccggg cgattacgac ggcgacggca aacaggacgt cgcggtctgg cgaccgtcaa
    17221 acggcacttg gtatgtgctc agaagcggga ctacgacttt acaggttcag caatggggac
    17281 aaaacggcga cacgcccgtc tggggcgatt acgacggcga tttacagtcg gatttcgctc
    17341 tggttcgcaa cagcggctca aatcgcctgt ggctggtttt gcaaagcaac ttcggctacg
    17401 gtttcgtcct gggctgcccg acgaccgcgc cgatttgctc gaacggcgtc cagtggggct
    17461 tgacgaccga caagctcgtg cccggcgatt atgacggcga cgcccgcacg gacgtggcgg
    17521 tctggcgacc gaccgacggc aactggtatt ggattagaag cgccagcaag acggtcggaa
    17581 acacgcccgg cgcgacgacc tccaatacac agtggggcgc aaacggcgac attccgcagc
    17641 cggctgatta cgacggcgat aagacgatgg actttgccgt tttccgttcc agccccgacc
    17701 cggcgcagaa tttctggtat attcgcaaca gcacgggcgg aaccttgacg gcgatagaat
    17761 ggggacaaca gggcgaccag cctgccacgt caccgtatag aattcaatag ctttttagcc
    17821 ctcgccggct aaattcgttg aaaggcttga gaaaaatttt tctcaagcct cttttctttt
    17881 ttctttttcg cccgctgcaa ccaattccaa atctgaccgc atcttttacc gcataacgga
    17941 cacgaattcc ccaggcgcaa aattgaaatc tgaaaaggaa aattgattta tgaagaaaaa
    18001 cggtttcctc gattcgatac gaatcgcatc gccctgctcg caggattggg acgagatgag
    18061 cggcaacgaa acggtgcgtt tctgcgagca ttgcgcattt gaagttaaaa acctttcggc
    18121 gatgaagcgc cgggaagcgc tgcgtctggt gcgccaatcg gaaggcagaa tctgcgtccg
    18181 ctacgtcaaa aatccccaaa ccgatgcgcc cgtctttgcc ggaaagcttt atcaaatcac
    18241 gcgccgcgcc ggagttgcgg cgggcgtttt gggagcgtcg ctgacgcttt cgacgctcgc
    18301 ctacgcgcag ggcgaagcgg tcaaccctct ttcaaaaccg acaattgaag cgtcgggcaa
    18361 aaatcaatcg gacagagaca aaaccaaaag cccgaccgga acgatttcgg gaacgacgac
    18421 cgactcgacc ggcgcgatta ttccgaatgt gacggttaag ctttcaaacg cagaatccgg
    18481 cgaatcgaga aacacgacga ccgatgaaaa cggtttatat gaatttaaag acgttttgac
    18541 cggaatatac aaagtcacag ccgcggcaac cgcgggcttt gcggcttacg agagtcaggc
    18601 aatcgaggtt tccgaaaatg cgaacattaa actggagctg tcgatgccgc tgagtctacc
    18661 gcggggcgcg tcctttacga tgggcttggt cgcgctggct cgtttggaat acgagcagcc
    18721 gctcttaaaa gccgtctccg gcgaaaattt tgaagaagtg aaagcgctag tcgccaaagg
    18781 cgcaaacgtc aacgcgaaag acaaaaatta cggcgaagcg accgcgctgc acgtcgccgt
    18841 cgagaacgat aatcgggaaa tcgccaaatt cctgctcgat atgggcgcga aaatcaatgc
    18901 gcgcgataaa cagcgccgca cgcctctgat gtcgatcgac gacgatacta agcccgaatt
    18961 ggtcagaatg ctgctcgacc atcgcgcgaa agtcaatgct ttggacgcgg aaggaaacac
    19021 ggctctgatt ttcgccgcgc aatacgagaa cgccgaggtt ctgcgcgttt tgattgatgc
    19081 gggcgcgcgc atcaacgcgc aaaataagga aggcaaaacg gctctgatgc tcgccgccga
    19141 aaacgactat tatacgagcg tcgaagtttt gctcgccgcc ggcgcggacg ttcacctgcg
    19201 cgataaagac ggcaaaaccg cgctggattt cgccgacgac gaaaaaatcc gcgacttgct
    19261 gaaatcttac aacgcgaagg aataaacggc agattcaaac aggacggaca ggatttacag
    19321 gataaattgt ttttcaaatc aagattccgg ttttattcat aacttttgaa taaaagaagc
    19381 ttctatcctg cctatcctgt ccatcctgtt tgaatctgcc gcagaattaa atcggcagtt
    19441 aattttcccg aaatcaaaac cagcgggatg ccgccgcccg gatgcgtcgc cccgccgaca
    19501 aaatagagat tttcaatttt tcgagccttg ttcggcgggc gcagaaaggc gctgaaaatc
    19561 ccgtttgacg agacgccgta aatcgagccg cgattggcgc gatatttttt ttcgaaatct
    19621 tcgggcgtga tgatttgctc gaaatcaatc gaattttcca aatcctttaa accggaatct
    19681 tccagtttcc cgacaatcaa atcgcggtaa tcgcgctttt ctttttccca atttgtctgc
    19741 gcgccggtgt gaggcgcgtt gattaaaacg aacaaatttt cacagccttc cggcgcttgc
    19801 gtccggtcgg ttcgggtcgc cgcgcagaca taaacggtcg gattccgcgc cggtcgcaaa
    19861 tctttgaaaa tcgcttcgaa ttccgcaaaa taatcgtccg agaaaaagat gttgtgatgc
    19921 gccagctgcg gaaagcgctt ttttgttccg agcagcagaa cgaaaccgct cgacgacggt
    19981 tcgagatttt cgatgcgcgt gttttcagat tcaatcagat tccggtaagt ctcgaccgcg
    20041 tcggaattgg cgatgacgaa atcgctttcc aggcgttcgc cgtttttgag ccggacgccg
    20101 attgcttttt tatttcggac gagaattttt tcgacctcgg aattcgtttt tatttttacg
    20161 tttaattcgt cagccagctt tccgagcgcg cgcgggattt catacattcc gcctttcgca
    20221 taccacgctc ccaaaccgaa ttcgacatag ggaatcagcg cgaaagtcgc cggagcctga
    20281 aaaggcgacg agccgttata ggtggcgaaa cgattgaaaa gctgacgcag cttcggcgat
    20341 gcgaaatagt ttttaacgtg cgcgtccagc gttcgcatcg aagaaatcgc cagcaaatcc
    20401 ctcagatatt tcgggcgcaa aagcttcggc aaatcgttca gactgtgcgc cagaaaagtt
    20461 ttttcggaaa cttcgtattt gcgtctggcg tcggctaaaa actttctgaa attttcgacc
    20521 tcgccgcgcg acgtcgttcg caaaatctcg ctttcggttt tcgcgagatt ctgcgaggcg
    20581 tcgaaacgcg ttccgtccga ccagaaataa cggcaaatcg gttcgaccgg aacaatgtcg
    20641 agataatctt ccacgcgttt tccggcgaaa acaaaaagct cttccagaac gtggcgcatc
    20701 gtcaaaaggc tcgcgcccgt gtcgaattta tagccgctcg cttcgacgat attcaccttg
    20761 ccgccgaccg cggcgttctt ctccagaacg gtcacggcga aacctttttt cgccagccgg
    20821 atcgccgcgc aaagcccgcc caagcctgcg ccgataatga ttgcgttttt gctcatttga
    20881 aaaatttaaa gggagacgcc cggcattaag cctgtatctc aatgttcatt ctatatatta
    20941 tctttaaatt tattctttgg attggcattt ttcgtgctaa aatccgtaga agattttgac
    21001 cgaagcttcc ctgaaaccga aaaacggcat ctaacaattt gaaactatga aattttgtcc
    21061 gacctgccag acacgctacg acgaagagat tttgcgtttc tgcaccaaag atggaacgcc
    21121 gctggttgac gaaaatcagc cgaattttac ggcaatgccg agcgaaagtt cgattgacga
    21181 cgacgatccg ggcgaacaaa ccgtcattcg ccgcaactcg ccgaacgttc cgccgcccgt
    21241 tcccgatttc gctgccgggc gaaccgtaca ggagcgcatc gtcgtgccga cctccgagcc
    21301 ggttcgcgct caaacctcgc cgccgccgaa tgtgccgccg cgaaaatcga gcacgaccgc
    21361 cgtcgttttg ctgacgctgc tcgggacgct cgcggttttg ggaatcggtt tcggcggctg
    21421 gtatctttta caaaatcgcg atgcggacga cgcgaaccgt aacatcaacg tcaacaccaa
    21481 cccggtcaat caaaatacaa acgtcaacgc ggacaattcg ccggcgaatt ttgactttaa
    21541 tatcaacacc aacacaaacg tcaacgccaa cgtcaatacc aatgtcaacg cgaatctgaa
    21601 aacgccgacg ccgacgccga ccagaacgcc gacgccgacg ccgaccagaa cgccgacgcc
    21661 ggaagccaac gttaacgtta atgctactgt cgtaaatgcc gcgcccgcca gcccgacgcc
    21721 gacgccgacg ccgcgcacga cgccgacgcc gagcccgcaa aacgtcaatg tcgggatgct
    21781 gaacagccgc gccgtcagcc tgccgaaacc ggcgtatccg ccgacggcgc gccagatgaa
    21841 cgcttccggc agagtgaccg tgcaggtgac gattgacgag gaaggaaacg ttttgtcggc
    21901 aaaagcaata aacggtcacg tgcttttgcg caatccggcg gaagccgccg cgcgccagtc
    21961 gaaattcaat cccgtccgcg tcggcggtca agccgtccgc gccagcggcg tgattctata
    22021 taattttatt aatcaataag tccaacaata caaaatgcaa aatattagga tttaagattc
    22081 aagattccga attaaatctt tcctttcatt ttgcactttg cattttgcgt taatatccta
    22141 ttgtttttcc gccatctcaa cggcgcggcg cgggtcgagc acgccccagc cttgacggtc
    22201 gacttcgtaa tgcggcaggc gctcggcggt cgaaatcaaa attcttttta tctgctgcgg
    22261 cgtcaaattc ggattagcct gaagcatctg cgcgacgacc gaggagacga tcggcgcggc
    22321 aaacgaagtg ccgtcgacgt atttgtaatg gcgcgtgatg acgttttcct gcctgatttt
    22381 aagggcgata atctggcgaa tcatatacgg cgggcggtcg agcgcggcgt ccagctcgcc
    22441 gtcgatgccc ggatttatct cgataatttt gtgcagctct tcgtcctttg ctttgtctaa
    22501 ttgttcgagc agcgccgctt gctgcgccgt cggcgtattc ggcagaatcg gcgcgggaat
    22561 ccagattgaa ggcgcgatga tttccggttt ctgcaagccg tcgaccgtcg gtccgtaaga
    22621 gctgcgatac attccgcgct ttgaacgctt gattgaatta ttatcgtcca gaccgccgac
    22681 ggcgatgcac gacggcgcgc tggctggcgg aaaaacggga tgcgtcgcca gatgtccggc
    22741 gttgccgacc gcacagacga ccgtgatgcc ttcgcgcacg cattcttcga ccgtctgcga
    22801 taagctgtcg tgcagatagc tcgcctcaaa atcgccgccc gccgaaatgt tgacgacgcg
    22861 aatgccgtat ttttcacggt tcgataaaac ccattccaga ccgtccagaa tattttgctc
    22921 ggtaatgcgt ccggttcgcg ccagttttat caggacgacg tttgcttcgg gcgcgattcc
    22981 gcgataaaag ccgtttgaca ggctgccgtt tcccgccgcg acgaccgagg tcatcatccc
    23041 gtgccagctg gcgacgtcgg attgaaacaa agatgcgtct ccgtcgcccg tcgcgctcag
    23101 attgtgataa gccagaatgc gattgctcgg cgcggtcaaa tcgatgtgcg gaaaaaatcc
    23161 cgaatcgaga aacgcgatgg tgattccgcc gcccgtatac ctttcgtcgg cgtcgagccg
    23221 gagcggcgtc gaaagaacgt gcgagccgtc cttctgcatc gccgcgtcct tgataatctg
    23281 ttccttgccg cgctcgaaaa ggcggacgca gcgcgcgcag acggcttgaa attcttcggt
    23341 gtcgggcgcg ttggcgcgaa tcaggttttg caaatcttct tccagcgcca ccagcggcac
    23401 gtattcgtct ttcgcctcgc gcgaacaaac cggacaggca ctggcgcccg ccgcaatgtc
    23461 ggttgtcatc ggatttcgag aaaaacctga agtttcgtgc agagtcattt tagagattta
    23521 agtttttcat aaacaaaagt taaaatcaat atcatcatag agagaggaag acacgagaga
    23581 tagaaaaaga gatttagccg cgaattacac gaataacact aactggtttt tatactgtta
    23641 atctcgtcca gcctgttgaa attctctctc tctctctctc tctgtgcctc cgtatcggcg
    23701 gtggtaaaaa attatgggta gaaaaaaaat cggcttggct ttatccggcg gcgcggcgcg
    23761 cggctacgcg catctcggcg ttttgaaagt tttagccgaa cacgatattc cgattgattg
    23821 tattgccgga acgagcgccg gttcgttcgc cggcgcggct ttcgccagcg gcttgagcgt
    23881 cgatgaaata atcgctttct cgaaaaaaat cagctggttt aatatgaccg gcttttccta
    23941 ctcgccgaag ggaattctgt cgaacgcgcc gatgggcaat tttatcaagc agaattttcc
    24001 ctttaaaacc ttcgaagaat tgccgattcc ttttgcggcg gtcgcctgcg atttggaaac
    24061 gggcgaagaa gtgattttga aagaaggcga tttgcctttc gccgtccgcg ccagctgcgc
    24121 gattccgggc gttttcgtcc cgctcgaaga cgccgacagc cgaaagctca tcgacggcgg
    24181 cgtggtcgcg cccgtgccga ccaaagccgt cagaaagctc ggcgcggaaa tcgttatcgc
    24241 cgttgacgtt atttcttccg gcgcgacata ttggggaaat ccgagcacgc ttctcggcgt
    24301 tttttttcaa tcggcgctga tgcttctcag aacggcttcc aaagcgcatc attaccgcgc
    24361 cgacgtcgtg attgtgccga aaatcgccca cctcagaccg gacgaaatcg gcaaaatgga
    24421 cgagtttatc aaagcgggcg aagaagccgc gctggaaaaa attgatgaaa taaaaaagct
    24481 ggtttttcca gaagataatt cagccgcaga ggacacagag aacgcagaga aagaaaattt
    24541 aaccacagat ttacacagat aaatacgaga ttttaacagg atgacacgat tatcaggata
    24601 atttaaaatt aataactttg agctttcttt tttatcttgt tcatcctgtt catcttgtta
    24661 aaattttctc tgtgttctct gtgtcctctg tggctaattt ccggtttctt actcgtttga
    24721 agaatcttcg atttccgcca atcccgtaaa agcgggcgtg tttatcggat gaccgtcgat
    24781 gcc
//
LOCUS       DASWNE010000690         1596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_693065, whole genome shotgun sequence.
ACCESSION   DASWNE010000690 DASWNE010000000
VERSION     DASWNE010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693065"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="VGB68_13575"
     CDS             complement(<1..1158)
                     /locus_tag="VGB68_13575"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEY0050319.1"
                     /translation="MSSPNNLEIQGSLHEHPLAELLVEAAAAHLSGSFRLNSQAQKAI
                     IYLRNGEIVFAVSNARRFRLFEMILQSGKLKPEQITGIPNFTNDQALSEALLAQNSLS
                     TFDIHRLFTRQVEGIVKSAIGWQEGEWTFSALVRIKESINYKIDAGKILFDYARALPP
                     DKIVRRFRSFQETFGVNPTHESPLQLATHEAFLLSRFEKSFLKIQEIVVLGGLPEAVT
                     MQTLYALWLGGFLYRQNWNAAFSERKISAILSAKLALKADEIAEAAKEEEEEIAAAAE
                     EKPRRFHQIKAEIARPSEKKEETKTPAPEIVKAADPAEELRQTEAYLKRVEDAVTLYQ
                     VLGVAPEVRTAEIKSTYFSLAKRFHPDLFHKETGTETHRRIQAAFTEIAHAY"
     gene            1343..>1596
                     /locus_tag="VGB68_13580"
     CDS             1343..>1596
                     /locus_tag="VGB68_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050320.1"
                     /translation="MLHDFTLIGKRVRLWQRAGESYGHVLMKALGWAMFAAEYPHLEI
                     EVKVGLRYKPDLIAARENGDFDFWGECGANSLRKTGWILK"
BASE COUNT          367 a          377 c          441 g          411 t
ORIGIN      
        1 gtaagcgtgc gcgatttccg taaacgcggc ttgaatgcgg cgatgcgttt ccgttcccgt
       61 ttccttgtga aaaaggtcgg ggtgaaaacg tttggcgagc gagaaatacg tgcttttgat
      121 ttccgccgtc ctgacctccg gagcgacgcc caaaacctga taaagcgtga cggcgtcttc
      181 gacgcgcttg agataagctt cggtctggcg gagttcttcc gccgggtcgg cggctttgac
      241 gatttccggc gcgggcgttt tcgtttcttc ttttttttcg ctcggtctgg cgatttccgc
      301 tttgatttgg tgaaaccggc gcggcttttc ttcggcggca gcggcaatct cttcttcttc
      361 ttctttcgcc gcttcggcaa tctcgtcggc tttgagcgcg agctttgccg acaaaatcgc
      421 ggaaattttt ctttcggaaa aagcggcgtt ccagttttgc cggtatagaa aaccgccgag
      481 ccagagagcg taaagcgtct gcatcgtcac ggcttcgggc aagccgccga gcacgacgat
      541 ttcctgaatt ttcaaaaaag atttttcaaa ccgcgaaagc aaaaacgctt cgtgcgtggc
      601 aagctgcaac ggcgattcgt gcgtcgggtt aacgccgaaa gtttcctgaa agctgcgaaa
      661 acgccggacg attttgtcgg gcgggagagc tcgggcgtaa tcgaaaagaa tttttccggc
      721 gtcgatttta tagttgatgg attccttgat gcgaaccagc gcgctgaaag tccattcgcc
      781 ttcctgccag ccgatggcgg attttacgat tccctcgacc tgacgcgtga aaagcctgtg
      841 aatatcgaat gtagaaagcg agttctgcgc cagcaaagct tcgctcaatg cctggtcgtt
      901 ggtaaaattc ggaattccgg tgatttgctc cggtttcaat ttgccgctct gcaaaatcat
      961 ctcgaacaga cgaaaccggc gcgcgttgga aacggcaaaa actatttcac cgttgcgcag
     1021 gtagataatc gctttctgcg cctgagaatt cagccgaaac gagccgctca ggtgcgccgc
     1081 ggcggcttcg accagaagct ctgccaaagg atgttcgtgc aaactgcctt gaatttcgag
     1141 attattcggg gaagacatct gaatttattc ggtaatttat ttggtaattt atgcggaaga
     1201 ttcggattga acgggataaa tcgctttaac cccgagtcaa taataacaga ttcggcggcg
     1261 aatagtgaat agtttatgtt aattttgtta tccggctcgg tcgattaatc acagacagag
     1321 aaaaaaataa aaggcaaaac ggatgctgca cgattttaca ttgattggaa agcgcgttcg
     1381 cttgtggcag cgagcgggcg aatcttacgg gcacgttttg atgaaggcgc tcggctgggc
     1441 gatgtttgcc gcggaatatc cgcatctgga aattgaagtg aaggtcgggc ttcgctataa
     1501 acccgatttg atagccgccc gcgaaaacgg cgattttgat ttctggggcg aatgcggcgc
     1561 gaacagcttg cgcaaaaccg gctggatttt aaaaca
//
LOCUS       DASWNE010000691         4017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_659391, whole genome shotgun sequence.
ACCESSION   DASWNE010000691 DASWNE010000000
VERSION     DASWNE010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659391"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /gene="tyrS"
                     /locus_tag="VGB68_13585"
     CDS             complement(<1..897)
                     /gene="tyrS"
                     /locus_tag="VGB68_13585"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEY0050321.1"
                     /translation="MTIDEQIEFIKKGAVDLIREEDLKAKLEKSVKTGEPLRVKLGAD
                     PTAPDIHLGHTVVIRKLKAFQELGHTAIFLIGDFTGMIGDPTGKSVTRPPLSPEEITA
                     NAETYKAQIFKLLDPERTDIRFNSEWMEKFGAADFVKLAAKTTVAQILERDDFEKRMT
                     EEKPIALHELLYPLVQGYDSVALEADVELGGTDQKFNLLMGRNLQRESEQEPQVILTM
                     PLLKGLDGVQKMSKSLNNYIGIDEAPNEMFGKVMSISDELMWEWYELLTDLTPSEIGS
                     LRSRCANGEENPRNVKVNLAKSI"
     gene            1031..1873
                     /gene="proC"
                     /locus_tag="VGB68_13590"
     CDS             1031..1873
                     /gene="proC"
                     /locus_tag="VGB68_13590"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEY0050322.1"
                     /translation="MENNLKKTNLAFVGCGVMAESIIAGLLRKNLVGAQQIFGSHPRA
                     NRRRELNEKYGIQVFEKNIEAVDAVKSSENSIVALCVKPQRLKGVLQELKGVVTPNQI
                     VLSIIAGARLETISEALGNQKIIRAMPYTPSQIGAGMTAWTCTEEISESEKARVKALL
                     TALGKELFVETENMIDMATSLSATGPTYIFMVMEALTDAGVHMGFSREVSKELVQETM
                     LGAVLFAMESHRHPAELRNMVTSPGGTSAEAIYQMEKGSLRTVLSKAVYAAYRRAVAL
                     GEKK"
     gene            complement(1875..2360)
                     /locus_tag="VGB68_13595"
     CDS             complement(1875..2360)
                     /locus_tag="VGB68_13595"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0050323.1"
                     /translation="MLEIRKAVEADKPPVWEIIKAVISTGDTYIFSPDSTEEKMLAYW
                     FAPDRKTYVALRENKIVGTFFLKDNQPDLGSHVANAGYMVAPEAKGKRVGRAMAEFSL
                     EEARRIGYRAMQFNFVVKSNEVAVRLWQALGFEIIGEIPEAFNHAQRGLTNAYIMYRK
                     L"
     gene            complement(2353..2763)
                     /locus_tag="VGB68_13600"
     CDS             complement(2353..2763)
                     /locus_tag="VGB68_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050324.1"
                     /translation="MSFLQNAKIGDVFSWDEVSEIHRIRNGIYQRGGRLVSLLTDFGK
                     INPCYPDFHGSSPDTVFYTGAGRRGDQRLDSFNRAMFNAVESKHAVPLFNKLKVGRWQ
                     FLGFWKVSEGKYIFDRRQDRMVWLFTLQRVDKNA"
     gene            3045..>4017
                     /locus_tag="VGB68_13605"
     CDS             3045..>4017
                     /locus_tag="VGB68_13605"
                     /inference="COORDINATES: protein motif:HMM:NF013604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEY0050325.1"
                     /translation="MKKILGILAATLFVGLLIFGLVANRVDANKTVFNTGSQDEHERA
                     KQISIEVLRGRSLARGIGNSEEFEVQKVHIDELRMAHTRFRQSVGGVPVWEGEAIVHL
                     KSDGEVSTVTDDLKEGISVSTKPTLSAGDAIEIANGLYNGSAKESAKPKVSLWIYRGE
                     TRDHLTWRIETPRIDGSKETAVPVDFIDAHTGERVFTYNNLQTGSGSSLYSGTVTINT
                     SSVGSTYYMEDLTRKMGTFNMNSTGNETYGTGGTQSRYTDTDDVWNTTIQRAGVDAHY
                     GAAKTYDYFLNVHGRNGIDGSGGPGTTTAAANSSISLIASRVHFGSSG"
BASE COUNT          979 a         1036 c         1022 g          980 t
ORIGIN      
        1 aatcgacttt gccagattca ctttcacgtt gcgcggattt tcctcgccgt tcgcgcagcg
       61 tgagcgcagg ctgccgattt ccgacggcgt caaatcggtc aaaagctcat accattccca
      121 catcagctcg tcggaaatcg acattacttt gccgaacatt tcattcggcg cttcgtcgat
      181 gccgatgtaa ttgttcagcg atttcgacat tttctgcacg ccgtccaatc ctttcaaaag
      241 cggcatcgtc aagatgacct gcggctcctg ctcggattcg cgctgcaaat ttctgcccat
      301 cagaagattg aatttctggt cggttccgcc gagctcgacg tcggcttcca gcgcgaccga
      361 atcgtagccc tgcaccagcg gataaagcaa ttcgtgcagc gcaatcggtt tttcttccgt
      421 catacgcttt tcaaaatcgt cgcgttcgag aatctgggcg acggtcgttt tcgccgccag
      481 cttgacgaaa tccgccgcgc cgaatttttc catccattcg gagttaaagc gaatgtccgt
      541 gcgttcgggg tcgagcaatt taaaaatctg cgctttataa gtttcggcgt ttgccgtgat
      601 ttcttcaggc gacaagggcg ggcgagtgac cgattttccc gtcgggtcgc cgatcattcc
      661 ggtaaaatcg ccgatgagaa aaatcgccgt gtgtccgagt tcctgaaacg cttttaattt
      721 gcggatgacg accgtgtgtc cgagatgtat gtcgggcgcg gtcgggtcgg cgccgagctt
      781 gacgcgcaga ggctcgccgg ttttgacgct tttttccagc ttcgctttta aatcttcttc
      841 gcggattaaa tcgaccgcgc cttttttgat aaattcgatt tgttcgtcta ttgtcatttc
      901 tttcgtgaat cgtgaatcgt aaatcggtta atagaaaatc attctttatc aaaatataaa
      961 acaagaacga taaataccaa aaagaccgga cttgcatttt tacgatttac gattcacgat
     1021 ttacaaattt atggaaaaca atctgaagaa aacgaatctg gcttttgtcg gctgcggcgt
     1081 gatggcggag tcgattatcg ccgggctttt gcgaaaaaat ctggtcggcg cgcagcagat
     1141 tttcggcagc catccgcgcg ccaatcgccg ccgggaacta aacgaaaaat acgggattca
     1201 ggttttcgag aaaaatatcg aggcggtcga cgccgtcaaa tcgagcgaaa attcgattgt
     1261 cgctttatgc gtcaaaccgc agcggctgaa aggcgttttg caggaattga aaggcgtcgt
     1321 cacgccgaat caaattgttc tttcaatcat cgcgggcgcg cgtctggaaa cgatttccga
     1381 ggcattgggc aatcaaaaaa taattcgcgc gatgccgtat acgccttcgc aaatcggcgc
     1441 gggaatgacc gcctggactt gcacggaaga aatttccgaa tcggaaaaag cgcgggtcaa
     1501 agccctgctg acggcgctcg gcaaagagct ttttgtcgaa accgaaaata tgattgatat
     1561 ggcgaccagc ttgagcgcga ccggtccgac ctatattttt atggtgatgg aagctttgac
     1621 ggacgcgggc gtgcatatgg gattttcgcg agaagtctcg aaggagctcg tgcaggaaac
     1681 gatgctcggc gcggtgcttt tcgcgatgga atcgcacagg catccggcgg agctcagaaa
     1741 tatggtcacg tcgcccggcg gaaccagcgc cgaagcgatt tatcagatgg aaaaaggcag
     1801 cctgcgcacc gttttatcga aagccgtcta cgccgcttac agacgcgccg tcgcgctcgg
     1861 cgaaaagaaa taaattataa ttttcgatac attatataag cgttcgtcag accgcgctgc
     1921 gcgtgattaa acgcttcggg aatctcgccg atgatttcaa agccgagagc ttgccataag
     1981 cggacggcga cttcattgct tttgaccacg aaattaaact gcatcgcccg gtaaccgatt
     2041 cgccgcgctt cttccagcga aaattccgcc atcgctctgc cgacgcgttt gcccttggct
     2101 tcgggcgcga ccatatatcc ggcgttggcg acgtgcgagc ctaaatcggg ctgattgtct
     2161 ttcagaaaaa aagtgccgac gattttgttt tcgcggagcg cgacatacgt tttccggtcg
     2221 ggggcaaacc agtaagcgag cattttttct tccgttgagt cgggagaaaa aatgtatgtg
     2281 tcgccggttg aaatgacggc tttgatgatt tcccaaaccg gcggcttgtc tgcttcgacg
     2341 gcttttctta tttcaagcat ttttatcgac tctctgcaaa gtaaacagcc agaccattct
     2401 atcctgtctc cggtcaaaaa tatattttcc ttccgacact ttccagaaac ccaaaaattg
     2461 ccagcgtccg accttcaatt tattaaaaag cggaacggcg tgttttgatt cgaccgcgtt
     2521 aaacatcgcc cggttgaatg aatcgagccg ctggtcgccg cgcctgcccg cgccggtgta
     2581 aaaaacggtg tcgggcgagc tgccgtgaaa atccgggtaa cacggattga ttttgccgaa
     2641 atcggtcaga agcgaaacca gcctgccgcc gcgctgatag attccattgc ggatgcggtg
     2701 aatttccgaa acctcgtccc acgaaaaaac gtcgccgatt ttagcgtttt gcagaaaaga
     2761 catcgattgt tattttaaac gcgaatcgac ggcgcgcgcg actttacact ttttttcgga
     2821 tttagtccac aatctttctt gacaccctaa ataagaaaag tttatagtaa ctttcacctt
     2881 cttgtttttg taattctccg acgaactttg cagaaacggg tctgcttact ctcaatatat
     2941 ttggaaaaaa atttattccg cgcccatttt agcgcttatt ttatatacgg cttgataata
     3001 ctatcggcaa tttccaaatt taaggctaag gagtaattta gagcgtgaaa aaaattctag
     3061 gtattttagc tgcgacgctg ttcgtcgggc ttctcatatt cggcttggtg gcgaatcgcg
     3121 tggatgcgaa taaaaccgta ttcaataccg gctcgcagga tgaacacgaa agagcaaaac
     3181 aaatcagcat cgaggttttg cgcggtcgct cgctggcgcg cggcatcggc aactccgagg
     3241 aattcgaggt tcagaaagtc catattgacg agttaagaat ggcgcacaca cgctttcgtc
     3301 aatcggtcgg cggcgttccc gtctgggaag gcgaagccat cgttcactta aaatcggacg
     3361 gcgaagtctc gaccgttacg gacgatttaa aagaaggcat ttcggtcagc acgaagccga
     3421 cgctgtcggc gggcgacgcg attgagattg ccaacggttt gtataacggc tcggctaaag
     3481 agagcgcaaa accgaaagtc agcctctgga tttatcgcgg cgaaacgcgc gaccatttaa
     3541 cctggcgcat cgaaacgccg cgcatcgacg gctctaagga aaccgccgtg ccggtcgatt
     3601 ttatcgacgc gcacacgggc gaaagagttt tcacctacaa caacctgcaa accggctccg
     3661 gctcgtcgct ttacagcggc acggtgacca tcaacaccag ttccgtcggc tcgacctact
     3721 acatggaaga tttgacccgc aaaatgggca ctttcaatat gaacagcacc ggcaacgaaa
     3781 cctacggaac gggcggcacg caatcgcgct ataccgacac ggacgacgtc tggaacacga
     3841 caatccagcg cgccggcgtc gacgcgcatt acggcgcggc gaaaacctac gattattttc
     3901 tgaacgttca cggtcgcaac ggcatcgacg gctccggcgg tcccggaacg accaccgccg
     3961 ccgccaacag ctcgattagc ttaatcgctt ccagagttca cttcggcagc agcggtc
//
LOCUS       DASWNE010000692         8591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_59040, whole genome shotgun sequence.
ACCESSION   DASWNE010000692 DASWNE010000000
VERSION     DASWNE010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8591
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59040"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1357
                     /locus_tag="VGB68_13610"
     CDS             <1..1357
                     /locus_tag="VGB68_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050326.1"
                     /translation="IEYPGRQGYFYLSGIEIKSFGYLIPTPTAEETIMIQTVLTFITR
                     VNPDKRARLEEILEQVRQNLDDNSYIPFASLTKLHFAGFVIFDDDAYGPYLVFENNFD
                     GSLDAHLDELIRYAGAGLHQIYDCCQEYAGGSYEAPELAAYMRARVVRPSAYHIGNVG
                     RSAARIKQESSLRDRIQDFFDELIAAGKGKEAPGSLHKYVQQFVRDDSSLAWACGDIA
                     PRQTTAERILPWIKIAAVALGAIILLPVLIPAAIVWVIVLRIMENRDSKIPQTATSSH
                     IRKLNEQEDRIVQNHLASITLVKPGWFRRWTLRIVLWLANLIARTSNKGELRGIPTIH
                     FAHWSLIDKGRRLLFLSNYDGSWMSYLDDFIDKASIGLTGVWSNTVGFPAAKFLVFEG
                     AQNEAGFKSFSRDSQTPAIVWYSAYPDLTVQNIDNDSSIREDLFTTPDETAAKNWLRL
                     F"
     gene            1374..2798
                     /locus_tag="VGB68_13615"
     CDS             1374..2798
                     /locus_tag="VGB68_13615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEY0050327.1"
                     /translation="MTKPEDEAMVKLEDEDIQGFMFSSYARYLHCAAYLLLRITDPEP
                     SRRWLGELTDKITTGKQKSKEERRKEIFALNIAFTSNSLKNLGFSEKELSGFSRPFQE
                     GMVTEARAKTLSDTGDSDPQNWNWGSLGNPVDILLMVFAVDENVLAEQLKLRRAEIVA
                     CGGLQEIIILPQAGRQPDMKEHFGYLDGVGQPVIEGSGKAQNQLRRTGHATVIKAGEF
                     ILGYENELGVLDGVPGAGEMPEFGVNGTYLVFRQMEQHVSAFWNFLEEATCRPDGTSD
                     PDARERLGAKIVGRWKSGAPVTRYPDRDPFQGEPQKTDENDFGYDEHDKYGFGCPIGA
                     HIRRANPRDSLPPNPETALKSAKRHRLIRQGRSYGDHAENVFIEDNKERGLYFICLNS
                     DIERQFEFTQQTWINNKKFGGLYDEVDPLIGRHKSDNLFTVQEDPLRTRVHNLRKFVT
                     IKGGSYFFMPGISALRYVAGLKNS"
     gene            2974..4980
                     /locus_tag="VGB68_13620"
     CDS             2974..4980
                     /locus_tag="VGB68_13620"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY0050328.1"
                     /translation="MNQNNPDIAASAISPAAMENPRHRLISARISPMVEMARWIFELY
                     NIPYAEERHAPVLHVLATRRAGGGAEVPVIVTSEAVWSGAREFLINFDSKSPPGNRLL
                     GENDSERNENIALADSLFALLLKQVRRYVYFHLLPEPGLVKPLVTEGVPRWQKLFVGL
                     FYPVWSRFMSSGLDFSSSLISDAPRDIEQAFRFVEDRLGDGRQFLAGERPGIVDIIFS
                     SLAAPVVLPSQYGAGLPAMNDLPAELRNFVSACRNRPAGQLVLDTYAARRTESQPLLN
                     YKKRTIGLGNLIFGPAVKVHIAKFLVRFAPRVVVGEFAVVSRWNDVKDVFKRDTEFLI
                     APLNAPNIEEINGPFVLGMDRSEVMLSEQRQMYAGLSAVDFEAVRAQVQAESKRLLAA
                     ARETGAGKIDVVNGYARLVAARTAISLFGVSGPSEAEYARVVRQIFYHAFLNIGADKD
                     IRNKALAASQDLRRWTLAEIARRQSTGEQKSDIMSALLALRGSDPEALDDDGVRRTLM
                     GMLVGAVDTTATAVAYCTAVLLSKPDLKNRALQDANDPERFVGWCWEALRFMPHNAVL
                     ARFAHTGTKIADKQLKRDTKVVINILGAMHDADTFPSPGQLNPERPLGNYFHFGGGLH
                     PCAGRAINAVQVPELVRQLLLHNASYAARPRFDGPFVDELLVQL"
     gene            complement(5107..5781)
                     /locus_tag="VGB68_13625"
     CDS             complement(5107..5781)
                     /locus_tag="VGB68_13625"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY0050329.1"
                     /translation="MGKVCLSFALTVFCVFSTFAQTDNKFSVNAERVSDINIAPDKKA
                     NTTEFVEAPDAQDKTEELEFQKTRWSAMADSVSFKTKSLLAGKSLGSFSTGDTVIDGY
                     ILESSVRHNVDPLLIFAQMSQESSFRKKATSHKGASGLMQLMPATAMRWGVKNIYNPK
                     QNIEAGVKYMRWLLDQFGGDVKLALAAYNAGEGSVKKYGNKIPPYSETINYVKRISTH
                     YDKIKM"
     gene            6405..7721
                     /gene="purB"
                     /locus_tag="VGB68_13630"
     CDS             6405..7721
                     /gene="purB"
                     /locus_tag="VGB68_13630"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEY0050330.1"
                     /translation="MIERYTLPEMGALWSQQNKFQKWLDVEIAVCEVHAEDGTIPAEA
                     LDEIKAKAAFTVERIGEIEKTTDHDVIAFTTNLAENIGEAARFVHYGLTSSDVVDTAN
                     ALLLRDSCDILLKKIDALLEVLRRRAFEFKETPQIGRTHGIHAEPTSFGLTWALWYAE
                     TERNRERLLRAKETIAVGKISGAVGAFAHLAPDVEERVCARLGLKAAPVSTQVIQRDR
                     YAEYLCTLAIIASTLEKIALQVRHWQRTEVREAQEKFKIGQKGSSAMPHKRNPILSER
                     ICGMARTVRANSIVGLENIALWHERDISHSSAERIVLPDSSATTDYILAKTANLLDGL
                     IVYPENMLKNLNLTRGLVFSGQLLLALTRKGVSREDAYVYTQRNAMRVWDEGGEYKDL
                     VLRDADIRAKLSAEEIEKVFDVRHYLRNVEKVYARVFEANRKGQAG"
     gene            complement(7755..8147)
                     /locus_tag="VGB68_13635"
     CDS             complement(7755..8147)
                     /locus_tag="VGB68_13635"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HEY0050331.1"
                     /translation="MQNMNGKTALGLDANVGALICYLGNLVCALGLIYSIIVLVTDKT
                     NKLPRFHAMQSILLSVVGIIAAVVINIIVGIAVAANSGILATLGSLIYLVFALAMLAA
                     VIFAAIKAFQGQIFKLPIIGDLADKWSN"
     gene            8268..8513
                     /gene="purS"
                     /locus_tag="VGB68_13640"
     CDS             8268..8513
                     /gene="purS"
                     /locus_tag="VGB68_13640"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEY0050332.1"
                     /translation="MKAKVYVTLKPSVLDPQGKAIHHSVELLGFESIKDVRQGKYFEV
                     QLADEMSETEARELASKIAKDVLANPVIEDYKVEIVL"
BASE COUNT         2159 a         2117 c         2336 g         1979 t
ORIGIN      
        1 tatagaatat ccggggcggc aaggatattt ttacctgtct gggattgaga ttaaatcttt
       61 cggttattta attccgacgc cgaccgccga agagacaata atgattcaaa ccgttctgac
      121 atttattacc agagtaaatc ccgataaaag agcgcggctt gaagaaatac tcgaacaagt
      181 ccggcaaaat cttgacgata attcatatat tccgtttgct tctttaacca aattgcactt
      241 cgccggtttc gtcatcttcg acgacgacgc atatggtccg tatctcgtat tcgaaaataa
      301 ttttgacggc tcgctggacg cgcatctgga tgaattaata cgatatgccg gcgcgggact
      361 gcaccaaata tacgattgct gtcaggaata cgcgggcggg tcgtatgaag cgccggagct
      421 ggctgcttat atgcgggcgc gcgtcgtccg accgagcgcc tatcatatcg gaaacgtcgg
      481 tcgcagcgcg gcgagaatca aacaggaatc ttcgctgcgc gaccggattc aggatttttt
      541 cgacgagctt atcgccgccg gtaaaggcaa agaagctccc ggctcgctgc ataaatacgt
      601 tcagcagttt gtccgcgacg attcgtcgct ggcgtgggcg tgcggcgata ttgctccgcg
      661 ccagacgacc gccgagcgaa ttctcccgtg gattaagata gccgccgtcg cgctcggcgc
      721 gattatcctg ctgcccgttt taattcccgc cgccattgtt tgggtaatag ttttgcgcat
      781 tatggaaaac cgcgactcaa aaattccgca gacggcgacc agcagccaca tccgaaaact
      841 gaatgaacag gaagacagga ttgtccagaa tcatctcgcc agcattacgc tcgtcaaacc
      901 gggctggttc cggcgctgga cgctgagaat cgtgctctgg ctcgccaatc tgatcgcccg
      961 cacctccaat aaaggagagc tgcgcggcat tcccaccatc catttcgctc actggtcgct
     1021 gatcgataag ggacgccgtc tgctctttct gagcaactat gacggaagct ggatgagtta
     1081 tctcgacgat tttatagata aggcgagcat tggtctgacc ggcgtctgga gcaatacggt
     1141 cggctttccg gcggcgaaat ttctcgtctt cgaaggcgcg caaaacgagg cgggctttaa
     1201 gtccttttcc agagacagcc aaactccggc gatagtgtgg tacagcgcgt atcctgattt
     1261 gaccgtgcag aatatcgata acgacagttc gattcgcgaa gaccttttca cgacgccgga
     1321 cgaaacggcg gcaaaaaact ggctgcggct tttctgattg gagcggggaa attatgacga
     1381 aaccggaaga tgaagctatg gtgaaactgg aagatgaaga tattcagggc tttatgttta
     1441 gctcatacgc gagatatctg cattgcgccg catatttatt actccgaata accgacccgg
     1501 aaccaagccg ccgctggctc ggcgagctga cagacaaaat cacgacgggt aaacaaaaaa
     1561 gcaaggaaga gcgcagaaag gaaatctttg ccctgaatat tgcctttact tccaatagtt
     1621 taaagaatct gggattttcc gagaaagagc tttccggttt ttcacgtcca tttcaggaag
     1681 gaatggttac cgaggctcgc gccaagactc tgagcgatac gggagacagc gacccgcaaa
     1741 actggaattg gggcagcctc ggcaatccgg tggatattct tctgatggtt tttgccgtcg
     1801 atgaaaatgt gctggctgaa caattaaagc tgcggcgcgc tgaaatcgtc gcctgcggcg
     1861 gacttcagga gataataata ctgccgcagg cgggcaggca gcccgatatg aaagagcact
     1921 tcgggtattt ggacggcgtc ggtcagcccg tcatcgaagg cagcggaaaa gcccaaaatc
     1981 aacttagacg aaccggacac gcaaccgtta ttaaagcggg agaatttatt ctcggatacg
     2041 aaaacgagct cggcgttttg gacggcgttc cgggcgcggg cgaaatgccc gaattcggcg
     2101 ttaacgggac gtatctggtt tttcgccaga tggagcagca cgtcagtgcc ttttggaatt
     2161 ttcttgaaga agcgacctgc cgcccggacg ggacgagcga cccggacgcc cgcgagcggc
     2221 tcggcgcgaa aattgtcggg cgctggaaga gcggcgcgcc cgtcaccaga taccccgacc
     2281 gcgacccctt tcagggcgag ccgcagaaaa cggacgaaaa cgatttcgga tatgacgaac
     2341 acgacaagta tggtttcggc tgtccgatag gcgctcatat tcgccgcgcc aatccgcgcg
     2401 attccttgcc gcccaatccc gaaaccgcgc tgaaatcggc aaaacgacat cgcctgatca
     2461 gacaggggcg ctcatacggc gaccacgccg aaaatgtttt tatcgaggac aacaaagaac
     2521 gcggcttgta ttttatttgt ctgaacagcg acatcgagcg ccagtttgaa tttacccagc
     2581 agacgtggat taacaataag aaattcggcg ggctttatga cgaggtcgac ccgcttatcg
     2641 gcagacacaa aagcgataat ttatttaccg ttcaggaaga cccgctgcgg acgcgcgttc
     2701 ataatctccg taaatttgtg acgataaaag gcggcagtta cttttttatg cccggaatca
     2761 gcgctttgcg atacgtggca ggacttaaga attcttaagc cgatttatat tattgcagtc
     2821 ggcagacttt aaaatataac tttttcaaat aagcccggcg tcttctttcg gtcgaaattt
     2881 tagtaaaggg caagatgtta ttgacggcaa ataggcgagc gaatttttaa attcagccga
     2941 ttccggcgca gacggaaaag acaggagtgt aaaatgaatc aaaataatcc ggacattgct
     3001 gcttccgcga ttagtccggc ggcgatggaa aatccgcgcc accgtcttat ttccgcccga
     3061 atcagcccga tggtggaaat ggctcgatgg attttcgagc tttataatat tccctatgcc
     3121 gaggagaggc acgctcctgt cctgcacgtt ttagcgactc gccgggcggg cggcggcgca
     3181 gaagtcccgg tcatagtcac ttccgaagcc gtctggagcg gagcgcgcga atttcttatc
     3241 aacttcgact cgaaaagccc gccgggaaac cgtttattgg gagaaaatga ttcggagcgc
     3301 aacgagaaca tcgcattggc ggacagtctt tttgcgcttt tgttgaaaca ggtcagacgc
     3361 tacgtatatt ttcacctgct cccggaaccg ggcttggtaa agccgctcgt cacggaaggc
     3421 gtcccgcgct ggcagaagct gttcgtcggg cttttttatc cggtttggag ccggtttatg
     3481 agcagcggac tggatttttc ctctagtctt attagtgacg cgccgaggga tatcgagcag
     3541 gcattcaggt ttgtcgaaga ccgtctcggc gacggtcgcc aatttctcgc gggcgagcgt
     3601 ccgggcatcg tcgatataat tttctcatct cttgccgctc cggttgtttt gccgtcgcaa
     3661 tacggagccg ggctgcccgc aatgaatgat ttgccggcgg agcttcgaaa ttttgtttca
     3721 gcctgccgca accgcccagc cggacaattg gttttagata cctatgctgc ccgccgaacc
     3781 gaatcgcaac cgttgttaaa ttacaaaaaa agaacaatcg gtttggggaa tttaattttc
     3841 ggtccggcgg taaaagtcca tatagcaaaa tttctggttc gcttcgcgcc gcgcgtggtc
     3901 gtcggcgaat ttgccgtcgt ctcccgctgg aatgacgtga aggacgtctt caagcgtgat
     3961 acggagtttc ttatcgcgcc gctcaacgct ccgaacatcg aagagattaa cggaccgttc
     4021 gtgctgggta tggaccgcag cgaagttatg ctcagcgaac agcgtcaaat gtatgccgga
     4081 ctttccgccg tcgattttga agccgtccgc gctcaggtgc aggcagaatc aaagcgcctg
     4141 ctggcggcgg cgcgcgagac gggcgcaggg aaaatcgatg tcgtcaacgg ctatgcgcga
     4201 ttggtcgccg ctcggacggc gatttcgttg tttggagtga gcggtccgtc ggaagccgag
     4261 tatgcgcgcg tcgtgcgcca aatattctat cacgcctttc tcaatatcgg ggcggacaaa
     4321 gacattagaa ataaggcgct ggcggcttct caggatttaa ggcgctggac gctcgccgag
     4381 atagcccgac gccaatcgac cggcgagcaa aagagcgaca ttatgtcggc tctgctcgct
     4441 cttcgcggct cagacccgga agcgctggac gacgacggag tgcgtcggac tttaatggga
     4501 atgctcgtcg gcgcggtcga tacgacggcg actgccgttg cctattgcac ggcggtgctt
     4561 ttaagtaaac cggatttaaa aaatcgggcg cttcaggatg cgaacgaccc ggaacgcttc
     4621 gtcggctggt gttgggaagc attaagattt atgccgcata acgcggtact ggcgcgcttc
     4681 gctcacacgg gaacaaagat tgccgacaag cagctgaaac gcgatacaaa agtagtaatc
     4741 aatatactcg gcgcgatgca cgatgccgat acatttccgt cgccggggca actaaacccg
     4801 gaacgtccgc tgggtaatta ttttcacttc ggaggaggtc tgcacccgtg tgccggaagg
     4861 gcgataaacg ctgtacaggt gccggaactg gtcaggcagc tattgcttca taacgcttct
     4921 tatgcggcaa gaccccggtt tgacggacca ttcgtggacg agcttctggt acagctgtaa
     4981 aaaagcatat tctcaaacct attcaggttc aaagacggca aaccgcggga aaggatttta
     5041 cacggctgtg gcgtggcgga aagccttcgt taagcgtccg gcgcggagca ttcaatcggc
     5101 agcgctttac atcttgattt tgtcgtaatg cgtgctaatc cgtttcacgt aattgatggt
     5161 ttccgaataa ggcgggattt tattgccgta tttctttacc gagccttcgc ccgcgttgta
     5221 agcagccagc gccagtttga cgtccccgcc gaattggtcc agcagccacc gcatatattt
     5281 tacaccggct tcgatgttct gcttcgggtt ataaatattt ttgacgcccc agcgcattgc
     5341 cgtagcgggc atcagctgca tcaagccgct tgctcctttg tgcgaggtcg ctttttttcg
     5401 aaatgaagat tcctggctca tctgcgcgaa aatcagaagc gggtcgacgt tgtggcggac
     5461 gctggattct aaaatatagc cgtcgattac cgtatcgccg gtcgaaaaag agcccagcga
     5521 tttgcccgcc aacagtgatt tggttttaaa ggacaccgaa tcagccatcg cgctccatct
     5581 ggtcttctga aactccaatt cttcggtttt gtcctgagcg tcgggcgctt cgacgaattc
     5641 ggtcgtattt gcctttttat cgggagcaat gttgatatcg ctgacccgct cggcatttac
     5701 ggagaattta ttatcggttt gagcaaaggt agaaaaaaca caaaaaactg ttaaagcgaa
     5761 agaaagacag acttttccca ttaagtaaaa attcctttat cggttaaagc gcggacgaaa
     5821 ataagaaaac atttctgcgc tgcgaaagtt aagttatcca ggggttgtta agagcgattg
     5881 cggtaaaact ttccaaagat tatttgaacg ggcttgctta aatcgtaatc attcggttac
     5941 tgacttagac ggcaaaattc cgtttccggt tgcagacaaa gttaatcttt gcggaaaagg
     6001 tcggtcgcgc cggagcggag cagcggcgga aaccgaagcg aatcggctcg aaagacagag
     6061 cgcgcggcgg gcagaccgcc aaaaaataag aatcagagac agctctcgcc gattttgtaa
     6121 attaagctcc cgtttaatat ttggtttttt cgcggagagt tgaaaaacca aaagcctccg
     6181 gttccgcaaa aaccaaaagc ttcgatattt gaaaaccgga cagtctccgc cggttatggc
     6241 aaaaacaatc tatcaagtcc gatttgcaat ttcaaaagac tgtatccgaa aatatatgtg
     6301 ttacttgcgg ttgagcgccg ggtttcggag cgacgaacgg caaaaggcag tcgcgcgaaa
     6361 tcattccgta aagcttatgt aaccgacggt aaaatatacc atttatgatt gaacgctaca
     6421 ctttgcccga aatgggcgct ctctggtcgc agcagaataa atttcaaaaa tggctcgacg
     6481 tggaaatcgc cgtctgcgaa gttcacgccg aagacggcac gattccggcc gaagcgctcg
     6541 atgaaatcaa agccaaagcc gcttttacgg tcgagcgcat cggcgaaatc gagaaaacga
     6601 ccgaccacga cgttatcgct tttacgacga atctggcgga aaacatcggc gaagcggcgc
     6661 gctttgtgca ttacggctta acgtcgagcg atgtcgtcga tacggcaaac gcgcttttgc
     6721 tgcgcgattc gtgcgatatt ttattgaaaa aaatcgacgc gctgctggaa gttctgcggc
     6781 ggcgcgcctt cgagtttaaa gagacgccgc aaatcggcag aacgcacggc attcacgccg
     6841 aaccgacttc attcggtctg acgtgggctt tgtggtatgc ggaaaccgag agaaaccgcg
     6901 agcggctgct gcgggctaaa gaaacgattg cggtcggcaa gatttccggc gcggtcggcg
     6961 cgtttgcgca tctcgcgccc gacgtcgaag agagagtttg cgcgcggctc ggcttaaaag
     7021 ccgcgccggt ttcgacgcag gtcattcagc gcgaccgcta cgccgaatat ctgtgcacgc
     7081 tggcgattat cgcttcgacg ctcgaaaaaa tcgcgcttca ggttcgccac tggcagcgca
     7141 cggaagtccg cgaagcgcag gaaaaattta agattgggca gaaaggctcg agcgcgatgc
     7201 cgcacaagcg caatccgatt ttgtcggaga gaatctgcgg aatggcgcgc accgtgcgcg
     7261 ccaattcgat agtcggtctg gaaaatatcg ctctctggca cgagcgcgac atctcgcatt
     7321 cgtcggcgga aagaatcgtc ttgcccgatt ccagcgcgac gaccgattac attctggcaa
     7381 aaacggcaaa tctgctggac gggctaatcg tttatccgga aaatatgttg aaaaatttga
     7441 acctgacgcg cgggcttgtc tttagcggtc agcttctgct ggctttgacg cggaagggcg
     7501 tctcgcgcga ggacgcgtat gtttacacgc agcgaaacgc gatgcgagtc tgggacgaag
     7561 gcggcgaata taaagatttg gttttgcgcg acgccgatat tcgggcgaaa ttgtcggcgg
     7621 aagaaatcga aaaagttttt gacgtgcgtc attatttgcg aaacgtcgaa aaggtttacg
     7681 cgcgggtctt tgaagcaaat aggaaagggc aagccggtta gactcgccct ctcgttctct
     7741 gatttcgtat caaattagtt tgaccattta tcagccaaat cgccgataat cggcagcttg
     7801 aagatttgtc cctgaaaagc tttgatagcg gcaaaaatta ccgctgccag cattgccagg
     7861 gcgaagacca gataaatcag cgagcccagg gtcgccagta tgccggaatt ggcggcaacc
     7921 gcgataccga cgataatatt gatgacgacc gcggcgatga tgccgacgac cgataaaaga
     7981 attgactgca tagcatggaa acgcggcagt ttgttggttt tgtccgtaac cagcacgatg
     8041 atgctgtaaa tcaaaccgag agcgcagacc agattgccca ggtaacagat tagcgcaccc
     8101 acattggcat ctagtccgag agcggtttta ccgttcatat tttgcatttt gaatctcctt
     8161 tttttgggaa aaaatttata attgaattgc cttacttggc gaaaatgata taacaagacg
     8221 aaaatagaaa caagatttgc gaaagtattt taggagtttt atttaagatg aaagcaaaag
     8281 tttacgtcac gctcaaaccg agcgtgctcg acccgcaggg aaaagccatt catcactcgg
     8341 tcgagttgct gggttttgaa agcatcaaag acgtccggca gggcaaatat ttcgaagtcc
     8401 agctcgccga cgaaatgtcc gaaaccgaag cccgcgaact cgcatctaaa attgcgaaag
     8461 acgttctggc aaatccggta attgaagatt ataaagtgga aatcgtttta tagccggcag
     8521 ccgtcagcta tcagccgtca gccagaaaat ggcaagctgg tagctgatgg ctgacggctg
     8581 atggctaaaa t
//
LOCUS       DASWNE010000693         1816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_306538, whole genome shotgun sequence.
ACCESSION   DASWNE010000693 DASWNE010000000
VERSION     DASWNE010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306538"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VGB68_13645"
     CDS             <1..236
                     /locus_tag="VGB68_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050333.1"
                     /translation="DGVWYRINSGTDSFTATQFGVSQDKPVPADYDGDGRADLGVFRP
                     SEANWYLLLSTAGYTGVQFGANGDIPTPNAFIR"
     gene            1046..1660
                     /locus_tag="VGB68_13650"
     CDS             1046..1660
                     /locus_tag="VGB68_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050334.1"
                     /translation="MNANVTKKSSASKFDSAGETDAALEKKLDEIEKRMLQILLDAKT
                     EVSVFEFREVFERYTGLTLAGQTDEVGENEREVFRIINHKNSELGAKCLHRRNRLRLE
                     FHQRLASRDFLKLLAKISFFEAEKLRRAALEFATLLNDVETRRAIENLFAESPQISHG
                     YAVKQGEKEVVWNAKEHKPPLAKPISVPLTSRSMIATANLSNKD"
BASE COUNT          451 a          422 c          433 g          510 t
ORIGIN      
        1 cggacggagt ctggtacaga atcaacagcg gcacggattc cttcacggcg acgcagttcg
       61 gagttagtca agacaagcct gtgccggcgg attacgacgg cgacggacgt gctgatttgg
      121 gtgtgttccg tccgagcgaa gccaattggt atcttctctt atcaactgcc ggatataccg
      181 gcgtgcagtt tggagcgaac ggcgatattc cgacgccgaa tgcttttatt cgttagaaaa
      241 taaaacgggg cagccaatcg tgactgcccc aagtagtttg ttggagaatg tatcctttcg
      301 agaagggctt gttactttca agacaaataa ataagttgcc ctgaatagtt acacgccgtt
      361 cgtctgcggt tgttgcgaaa agaattgttc gatggcgctg cgggtttcga cgcgcttgcg
      421 ctgttgaaat tccgtgaccc agctggagac gtttgaaacc atttcccggg ctgctgcttt
      481 agttgatttt ttttcgattt ccgctgattg cggtgccgcc gctgctactg cctgcgttcc
      541 ttttttgatt acttttattt tccctttggt ggtcatgatt gctgtttcct ctttttattt
      601 ataaatcctt aatttattag ttcttgaaag atttgtttat tccctcgctc gtttggttag
      661 catttctcgt gccactcatt agacgatgcc cggaaatttt tttgcattct gcaaagctca
      721 taaataaaat tttgcgcttt gctaaattac gactaacgtc aaacgggtct tacaagttcc
      781 cggaattcct gcaaaaacac cgattatttc agcgtttccc aagagaacgc gccgccggaa
      841 aaaacttgca aaacggctgg cataaacctt gccattttct caaaatgtga tgaatgcgtt
      901 cgagattttt ataatttatc ttgcctgcgg tgcaccattc ggtgtctatt tcttttttca
      961 gaatcgtcat aagtcggttt tttcgactgc gctgctgaaa tcttttctta cagtcgttgt
     1021 ttggattcct tacgctttgc ggttattgaa cgcaaacgtt acaaaaaaat cgtctgcttc
     1081 aaaattcgat tccgcgggcg aaacggatgc ggcgctggag aaaaagctcg atgaaatcga
     1141 aaaacgaatg ctgcaaattc ttttggacgc caagacggaa gtttccgttt ttgaatttcg
     1201 cgaagtgttc gaacgctata cgggtctaac tttagccgga cagacggacg aggtcggcga
     1261 aaacgagcgg gaagtcttca ggattatcaa tcataaaaac tccgagctcg gcgcaaaatg
     1321 tttacatcgg cgcaaccgtt tgcggctgga atttcatcaa aggcttgcca gccgcgattt
     1381 tttgaaattg ctggcgaaaa tttctttttt cgaagcggaa aaacttcgcc gcgcggcgct
     1441 cgagtttgct acacttttaa atgacgttga aactcggcgc gcaatcgaaa atctatttgc
     1501 cgaaagcccg caaatcagtc acggttatgc cgtcaaacaa ggggagaaag aagtagtatg
     1561 gaacgccaaa gaacacaaac cgccgctcgc caagccgatt tccgttcctt taaccagccg
     1621 ttcaatgata gcgacagcca acttgtccaa caaagattaa acacgcttct ggcggcgaat
     1681 gccgaaatcg agcgcgaaaa aacgatgttt cgcaccgagc gcgagcatct ggaagccgaa
     1741 ctgatgaaaa gcccgctcgg caccgaaaaa gcgctagcct atttcggcgc gatgctcggg
     1801 ctttttccgc cgtttg
//
LOCUS       DASWNE010000694         3681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_374871, whole genome shotgun sequence.
ACCESSION   DASWNE010000694 DASWNE010000000
VERSION     DASWNE010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374871"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /locus_tag="VGB68_13655"
     CDS             <1..1248
                     /locus_tag="VGB68_13655"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEY0050335.1"
                     /translation="VVDKLRMLLDITKTISRSLDLDEVLTLVMDTLDSVIHYDAAGIY
                     LIEHDQTGENPYIFKSKVYRGYEITFELVEPRLKLGEGFIGYVAQTGESIISHDVSQD
                     ARYFHARRRTKSEMVAPIVSNEEVIGVFDLESDELNAYDQDDLALLQLLASQVAIIIE
                     KVQLHEVLVEKKRLQGQLEVARQVQLELLPDEDPHLEGFDISAYIFPTEEVSGDYYDW
                     VKIFDDQIGIIVADAVGKGIPAALLMAFLRASLRSGVQIGYAPHIALAKVSNLLWDSI
                     EDHQFITAIYGILDDTNKTFVFSNAGHNPPVLVKATGEYKFVEYGDLPLGMFRGMRYH
                     QHFLRLDRGDILVIYTDGITEAACESTEEYGRKRLAERIVAGIDLTAKELINFIYQDV
                     LSFSSSKCLDDDGTLFVVKRLDL"
     gene            1420..1914
                     /gene="rimP"
                     /locus_tag="VGB68_13660"
     CDS             1420..1914
                     /gene="rimP"
                     /locus_tag="VGB68_13660"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEY0050336.1"
                     /translation="MNKLVIAEQVRAIAAEAAGKNNLELVHVDVVGTAKSPTVRVFID
                     KPGGVSVGDCTQVSRQMEAVLDAEDFISSAYVLEVSSPGLERELYSLQDFEKFGGNLA
                     RVRTHEPINGQRNFRGRIKGIDGENIIFEDKTNGEVSFPYDLVAKANLEIDLEQEFKR
                     NEKR"
     gene            1972..3483
                     /gene="nusA"
                     /locus_tag="VGB68_13665"
     CDS             1972..3483
                     /gene="nusA"
                     /locus_tag="VGB68_13665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEY0050337.1"
                     /translation="MTSSIGQSIDALCTEHGLDRNMIIEAMKDAVRAAARKQFKAQDK
                     TGESIQVDWNNDEGMIEISAQKEVVEAVENPQTELSLVEAQEMAGDEIEIGDMLLLPL
                     PTEDLGRIAAQTAKQILVQKVREAVREKVYDEYIDRKGELINGTVKRFERGDMIIDLG
                     NNLEAVLPRREQVRSENWNQGERIRVVIAEVTKDPKGQQIIASRASAELLKRLFEMEV
                     PEIYDDTVVIKSAVREPGDRAKIAVASNEKDVDPVGACVGMKGSRVQSIIKELRGEKI
                     DIIEWSEEPSVFAANALSPAKVSQVRIVDINKRKMEVIVVEDQLSLAIGKKGQNVRLA
                     TRLVGWDIDIVSEEVIKKEIAQQMGKMMASGEAVPVSALEGVTPAQANDLKDAGVNDV
                     ETLAAMSVDDLVDMLDTSLDQAEKILTSAQAVVKAKEAAATAPADEQDEQAGDESIEA
                     EASAEEASETEESSDIEESSEAGDSSVEEASEAEESDVEETGSDTVSAEEAKQ"
BASE COUNT          960 a          900 c         1055 g          766 t
ORIGIN      
        1 gtcgtcgata agctgcggat gcttttggac atcaccaaaa ccatcagccg ctcgctggat
       61 ttggacgagg ttttgacgct cgtgatggac acgctcgatt cggtgattca ttacgacgcg
      121 gctggaattt atcttatcga gcacgaccag acgggcgaaa acccgtatat tttcaaatcg
      181 aaagtttatc gcggctacga gataacgttt gagctggtcg agccgcgtct gaagctcggc
      241 gaaggcttta tcggatatgt cgcgcagacc ggcgaatcga ttatcagcca cgacgtcagc
      301 caggacgcgc gctattttca cgcccgccgg cgaacgaaat cggagatggt cgcgccgatt
      361 gtttccaacg aagaagttat cggcgttttc gacctcgaaa gcgacgagct taacgcttat
      421 gatcaggatg atttggcgct tctgcagctg ctcgcctcgc aggtcgccat catcatcgaa
      481 aaagtgcagc tgcacgaagt gctggtcgaa aagaaacgcc tgcagggaca actcgaagtc
      541 gcgcggcagg ttcagctcga gcttctgccc gacgaagacc cgcatctgga aggcttcgac
      601 atcagcgcct atatttttcc gaccgaagaa gtttccggcg attattacga ctgggtgaaa
      661 attttcgacg accaaatcgg cattatcgtc gccgacgcgg tcggcaaggg aattccggcg
      721 gcgctgttga tggctttcct gcgcgcttcg ctgcgttccg gcgtccaaat cggctacgcg
      781 ccgcacatcg cgctggcgaa agtcagcaat ctgctgtggg attcaatcga agaccatcaa
      841 tttatcaccg cgatttacgg catcctggac gatacgaaca aaaccttcgt cttctccaac
      901 gccggtcaca atccgcccgt tctggtcaaa gcgaccggcg aatacaagtt cgtcgaatac
      961 ggcgatttgc cgctcggaat gtttcgcggg atgcgctacc accagcattt cctgcgcctc
     1021 gaccgcggcg atattctcgt catctacacg gacggcatca ccgaagccgc ctgcgaaagc
     1081 accgaagaat acgggcgcaa gcgcctcgcc gaaagaatcg tcgccggaat cgacctgacg
     1141 gcaaaagaac tgataaattt tatctatcag gacgttttga gcttctcgtc cagcaaatgc
     1201 ctcgacgacg acggaacgct ttttgtcgtg aaaagattag acctttagaa aatagaatat
     1261 tattagcttt gcgccgaaat tatcaggacg ccgccgggca taatccttcc ttgcttttta
     1321 cccgcacttt cgtgtatgat attcgtttgc tgaaaggcgc aggggacgtt ctcggtgggt
     1381 tcgcgcccac tttttatttg ggcgatagag tttgggaaaa tgaataaact ggtcatagcg
     1441 gaacaggttc gggcgattgc cgccgaggcg gcggggaaga ataatctcga actggttcac
     1501 gtcgatgtcg tcgggacggc gaagagcccg acggtgcggg tttttattga caagccgggc
     1561 ggcgtttcgg tcggggattg cacgcaggtc agccggcaga tggaagcggt tttggacgcg
     1621 gaggatttta tctcttcggc ttatgtgctg gaagtttctt cgccgggctt ggagcgcgag
     1681 ctttacagcc tgcaggattt tgaaaaattc ggcggaaatc tggcgcgcgt tcggacgcac
     1741 gagccaatta acgggcaacg aaattttcgc ggaagaatca aaggtattga cggcgaaaat
     1801 atcatcttcg aagacaaaac caacggcgaa gttagtttcc cttacgacct cgtagcaaaa
     1861 gctaatttag agattgattt agaacaagag ttcaaacgaa acgaaaaacg ataaacggaa
     1921 aacggggaat atttttccac tttccatttt taatttttca ttggcgaaat tatgacatca
     1981 agcatcgggc agagcattga cgcattgtgc acggaacacg gtctcgaccg taatatgatt
     2041 atcgaagcga tgaaggacgc ggttcgcgcg gcggcgcgca agcagttcaa agcgcaggac
     2101 aaaaccggcg agagcatcca ggtcgattgg aacaacgacg agggaatgat tgaaatttcc
     2161 gcgcagaagg aagtcgtcga agcggtcgaa aatccgcaga ccgagctttc actcgtggaa
     2221 gcgcaggaaa tggcgggcga cgaaatcgaa atcggcgata tgctgcttct gcctctgccg
     2281 accgaagatt tggggcgcat cgcggcgcag accgccaagc agattctcgt gcagaaagtg
     2341 cgcgaagccg tccgcgaaaa agtttacgac gaatatatcg accgcaaagg cgagttgatt
     2401 aacggcacgg tcaagcgctt cgagcgcggc gatatgatta tcgacctcgg caacaatctg
     2461 gaagcggttt tgcccagacg cgaacaggtc agaagcgaaa actggaatca gggcgagcgc
     2521 atccgcgtcg ttatcgccga ggtcacgaaa gacccgaaag gacagcagat tatcgcttcg
     2581 cgcgcttcgg cggaactttt gaaacgactg tttgaaatgg aagtgccgga aatttacgac
     2641 gacacggttg tgattaaatc cgccgtgcgc gagccgggcg accgcgcgaa aatcgccgtc
     2701 gcttcgaatg aaaaggacgt cgacccggtc ggcgcgtgcg tcggtatgaa aggctcgcgc
     2761 gtgcagtcga ttatcaagga attgcgcggc gaaaaaatcg acattatcga gtggtcggaa
     2821 gagccgtccg tttttgcggc aaacgctctg agcccggcga aagtcagcca ggtgcgcatc
     2881 gtcgacatca acaagcgcaa gatggaagtc atcgtcgtcg aagaccagct ttcactggca
     2941 atcggtaaaa aaggtcagaa cgtgcggttg gcgacacggc tcgtcggttg ggatatagat
     3001 attgtcagcg aagaagtcat caagaaagaa atcgcccagc agatgggcaa aatgatggct
     3061 tcgggcgaag ccgttccggt ttccgcgctc gaaggcgtca cgccggcgca agccaacgat
     3121 ttgaaagatg cgggcgtcaa cgacgtcgaa acgctggcgg cgatgtcggt cgacgatttg
     3181 gtcgatatgc tcgacaccag cctcgaccag gctgagaaaa ttttaacttc ggcgcaggca
     3241 gtggtaaagg ctaaggaagc cgctgccacc gctccggctg atgagcagga cgaacaggcg
     3301 ggcgacgaat caatcgaagc cgaagcttcg gctgaagaag cttcggaaac cgaagaatcg
     3361 tcagatatcg aagaatcttc agaggctgga gattcaagcg ttgaagaagc ttcggaggct
     3421 gaagagtcgg acgttgaaga aaccggaagc gatacggttt cggcggaaga agcaaaacaa
     3481 tgatagtgaa aacagggaac gcggtgcggt gaaaagttaa ataattttcc gctttctctt
     3541 cggcaaaagt caccgcgttc agaaccggag agaaagcaac cattatgact ctattaggca
     3601 ataaagttcg tgtttacgac ttagccaaag aattaaaaca agacacgaag cgcatcatcg
     3661 aagaattgcg gcgcgaaggt g
//
LOCUS       DASWNE010000695         2532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_162780, whole genome shotgun sequence.
ACCESSION   DASWNE010000695 DASWNE010000000
VERSION     DASWNE010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162780"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VGB68_13670"
     CDS             <1..494
                     /locus_tag="VGB68_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050338.1"
                     /translation="DVSENKNTSYYIFMAPLIALLFGFGILFVVNRFLLKDRWSLSLI
                     GRISLALMLMVTATAHFTATDLMVELMPEFLPLKRETVYFTGVIEFLAVAGLVIDKTS
                     RATSILLIIFFLAVLPANIVGSLKQVNLGGMENGAAYLFFRVPLQLFFILWAYYFGVR
                     INK"
     gene            complement(512..1936)
                     /locus_tag="VGB68_13675"
     CDS             complement(512..1936)
                     /locus_tag="VGB68_13675"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0050339.1"
                     /translation="MKKQFFSFLILISFVLSVSAQKAQVAKPDLTERNLRKHVSYLAS
                     DKLEGRRTGEQGATFAAGYVANMFAQYKLKTGFRAANGKMNFLQPFSYLPRDAKGEIP
                     ADAKPAEAYNVIGVLEGTDAVLKNEVVIIGAHYDHLGKGGAGSLAANSVEIHHGADDN
                     ASGVAAMLELARQLAGEKKNKRTIVFAAFGGEEEGLLGSKFYVNNPPFALDKTVAMIN
                     LDMVGRLHENKLTVGGIGTASEWRELTLRIAINMPTLGMFQTPEASKSKAEDNSKLST
                     PIASSQTPILSAVPPKIPVKDQAFNLQLNEDGFGPSDHSSFYAKQIPVLFFFTGTHAD
                     YHKPTDTAEKINYEGLLKITNYISEIVKTIDQNPKRPTYAVAKSSGTGGRTGFNVTIG
                     VVPSYAESSGGLLLDGVRDGSPAAAAGIKAGDKIVKFAGKEIRNISDYTSVLGELKAD
                     TEYEIEVVRGSERLTLKVKPAARK"
     gene            complement(1936..2406)
                     /locus_tag="VGB68_13680"
     CDS             complement(1936..2406)
                     /locus_tag="VGB68_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050340.1"
                     /translation="MKAIFSALLFCFLCVVPAFSQEAQIFDSFAALNCDDYLGRMDNV
                     FVAARANPSTGFYVLIYEGDELAYNARKNEIELMLPAYGSADAKIRSIKKYMSIRKLP
                     VNRFTFVKAGFRENAAVEIWTVPSGAPTPKPTPTITKMKYRKGKPVGFCTDCCG"
BASE COUNT          549 a          667 c          629 g          687 t
ORIGIN      
        1 cggatgtatc cgaaaacaaa aatacatctt actatatctt tatggctcct ttgattgctt
       61 tgctgttcgg tttcggaatt ttattcgtcg tcaatcgatt tctgctgaaa gaccggtggt
      121 cgctttcgct aatcggcaga atttcgctgg cgctgatgct gatggtgacg gcgacggcgc
      181 attttacggc gaccgatttg atggttgaac tgatgccgga atttctgccg ctgaaaagag
      241 aaaccgttta ttttacgggc gtaatcgaat ttctggcggt cgccgggtta gtaatcgaca
      301 agacgtcgcg cgcgacgtcg attctgctga ttattttctt tctcgccgtt ctgcccgcca
      361 atatcgtcgg cagcctgaaa caggtaaatc tgggcggaat ggaaaacggc gcggcgtatc
      421 tgtttttccg cgtgccgctc cagcttttct ttattttgtg ggcgtattat ttcggcgtca
      481 gaatcaataa ataaagcttt gcttcagcct cttatttgcg cgccgccggt tttaccttca
      541 aagtcaatcg ctcagagccg cgcacgactt cgatttcgta ttccgtgtcg gctttcaact
      601 cgcccagtac ggacgtgtaa tccgagatat tgcggatttc ctttcccgca aatttgacga
      661 ttttatcgcc cgctttgatt ccggctgcgg ctgccggcga gccgtcgcgc acgccgtcga
      721 gcagcaagcc gccgctcgat tcggcataac tcggcacgac gccgatggtc acgttaaaac
      781 ctgttctgcc gccggttccc gaagatttgg cgacggcgta agtcgggcgc ttcgggtttt
      841 ggtcgattgt tttcacgatt tccgaaatgt aattcgtgat tttcaataaa ccttcgtaat
      901 tgattttttc cgctgtgtcc gtcggtttgt gataatcggc gtgcgttccg gtgaaaaaga
      961 atagaaccgg aatttgttta gcgtagaatg atgaatggtc actcggtccg aagccgtctt
     1021 cgttaagttg cagattaaaa gcctggtctt tgacaggtat tttgggcgga actgctgaaa
     1081 gaataggcgt ttgcgaagat gcaatcggcg ttgacaattt tgaattgtcc tcagctttac
     1141 ttttagacgc ttccggcgtt tgaaacattc cgagcgtcgg catatttatc gcaattcgca
     1201 gcgttaattc ccgccactcg ctcgccgtgc cgatgccgcc gaccgttaat ttgttttcgt
     1261 gcaaacgccc gaccatatcc agatttatca tcgccacggt tttatcgagc gcgaaaggcg
     1321 gattgttgac gtagaatttc gagcccaaca agccttcttc ctcgccgccg aaagctgcga
     1381 aaacaatcgt ccgcttgttt ttcttttctc cggcgagttg gcgcgccagt tccagcatcg
     1441 ccgctacgcc cgaagcgttg tcgtccgcgc cgtgatgaat ttcgacggaa tttgccgcca
     1501 ggctgcccgc gccgcctttg ccgagatggt cgtaatgcgc gccgataatg acgacttcgt
     1561 ttttcaaaac cgcgtccgtg ccctccagaa cgccgatgac gttgtaggct tcggcgggtt
     1621 tcgcgtccgc cggaatttcg cctttcgcgt cgcgcggcaa gtaggaaaac ggctggagaa
     1681 aattcatctt gccgttggcg gcgcgaaaac ccgttttgag cttgtattgc gcgaacatat
     1741 tcgccacgta accggcggcg aaagtcgcgc cctgttcgcc cgtccgccga ccttcgagct
     1801 tgtcgctcgc cagatacgac acgtgcttgc gcagattccg ctcggtcaaa tccggcttgg
     1861 cgacctgcgc cttttgcgcc gaaacgctca gaacaaacga gattaaaatt aaaaaactaa
     1921 aaaattgttt tttcatcatc cgcaacaatc cgtacaaaat cctacgggct ttccttttcg
     1981 atacttcatt ttcgttattg tcggcgtcgg tttcggcgtc ggcgcgccgc tcggcacagt
     2041 ccagatttca accgccgcat tttcgcgaaa tccggctttg acaaacgtaa atctattgac
     2101 gggcagtttc ctaatcgaca tatatttttt tatcgagcga atcttcgcgt ccgccgagcc
     2161 gtaagccgga agcatcaatt caatttcgtt tttacgagcg ttatatgcca gctcatcgcc
     2221 ctcgtaaatt aatacgtaga atccggttga cggattagcg cgcgcagcaa cgaatacatt
     2281 atccattctg cccagataat cgtcgcagtt aagggctgca aagctgtcga atatctgcgc
     2341 ctcttgcgaa aaagccggaa cgacgcacag aaaacaaaaa agcagagccg aaaatatcgc
     2401 tttcataatt tactaatcga cccagtcggc gataaaaaca ttcgtgtcgc ctgcccgcgc
     2461 cgcgtttcgg ttcgaggcga aaacgagctt tttgccgtcg ggcgaaaaca tcgggaaacc
     2521 gtcgaaaact tc
//
LOCUS       DASWNE010000696         1653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_271980, whole genome shotgun sequence.
ACCESSION   DASWNE010000696 DASWNE010000000
VERSION     DASWNE010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271980"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VGB68_13685"
     CDS             complement(<1..194)
                     /locus_tag="VGB68_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY0050341.1"
                     /translation="MFCIKCGARNSDEAIYCQKCGSLLEAEEETRVARQPLKEAVRDE
                     PEPEEKEIFSVRPTLMFIYAG"
     gene            complement(385..1218)
                     /locus_tag="VGB68_13690"
     CDS             complement(385..1218)
                     /locus_tag="VGB68_13690"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY0050342.1"
                     /translation="MKKLSFFFTISCLLVINSFAQTRERRVFDNFDTVSGVQIIAPPQ
                     PATVVAPKNNPKKLIRKTGQSKASVQDGLALQNVSQPLNKLAMAAGNNLGGFTTGDAL
                     IDSYIVESSIRYNVDPLLIYAQMHQESSFKRKAVSYKGASGLMQLMPATARRFGVTEI
                     FNPKQNIDAGIKYMRWLLNTFNQDLRLALAGYNAGEGAVMKYGWQIPPYNETQEYVRR
                     ITSRYNSIRNPNYIARTVKKVSATEAAKLEQKESAPLTVYEPEPLAVRLPDGRMRLVS
                     R"
     gene            1449..>1653
                     /locus_tag="VGB68_13695"
     CDS             1449..>1653
                     /locus_tag="VGB68_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEY0050343.1"
                     /translation="MLYLSQILNRPIFDAEGEKIAAIRDVIVRYGREDYPPLVGLVAR
                     YRRRNFFMSRSRISALGESGAKLR"
BASE COUNT          383 a          402 c          410 g          458 t
ORIGIN      
        1 ccggcgtaaa taaacatcag cgtcgggcga acggaaaaga tttctttttc ttcaggttcg
       61 ggctcgtccc tgactgcttc tttcagcggt tggcgcgcga cccgcgtttc ttcttcggct
      121 tccagcaagc tgccgcattt ctggcagtaa atcgcttcgt cggaatttct cgcgccgcat
      181 tttatgcaaa acataatgtt taagtcgagc gtcgagagcc gcaagtcgag atgattttga
      241 ctctttacgc tcgactcttc actcctgact gtttaagcat catacaagaa acatttattt
      301 catacaaaaa gacggtcggc gaactttcgc cgaccgtcaa cagtcctttc cggactaagg
      361 aggataagag actccgtaag aaatttaccg gctgaccagc cgcatccgtc cgtcgggcag
      421 ccgaaccgcc agcggttccg gttcgtaaac cgttaacggc gcggattctt tctgctccaa
      481 tttggcggct tcggtcgccg agactttttt caccgtgcgg gcgatgtaat tcggatttcg
      541 gatggaattg taacgcgatg tgatgcggcg cacgtattcc tgcgtctcgt tatacggcgg
      601 aatctgccag ccgtatttca tcaccgcgcc ttcgccggcg ttatagcccg ccagcgcgag
      661 ccgcaaatcc tgattgaaag tattgagcag ccagcgcata tattttatgc ccgcgtcgat
      721 gttctgtttc ggattgaaaa tttccgtcac gccgaaacga cgcgccgtcg ccggcatcag
      781 ctgcatcaaa ccgctcgcgc ctttgtagga aaccgctttg cgcttaaagg aagattcctg
      841 gtgcatctgt gcgtaaatca gaagcgggtc gacgttgtat cgaatgctgg attcgacaat
      901 ataggaatca atcagcgcgt cgcccgtggt aaatccgccg aggttgtttc ccgccgccat
      961 cgcgagtttg ttgagcggct gagagacgtt ttgcaaagcc aagccgtcct gaacgctcgc
     1021 tttgctctgt ccggtcttcc tgattaattt tttagggtta tttttcggcg cgacgaccgt
     1081 cgccggctgc ggcggagcga tgatttgaac gccgctcacg gtatcgaaat tatcgaaaac
     1141 gcgtctttcg cgcgtctgag caaaagaatt aataaccaga agacagctaa tcgtaaagaa
     1201 aaaagacagt tttttcatct atggaatttc ctaatgtaaa ttgggtagag cttgggttga
     1261 agtggttttt gatattttga tactcgacaa aaagagcatc tattttatta gagattgcca
     1321 aagctcggag gtttccacaa atttttacaa tatttaaaaa gtttttacaa aagggcggat
     1381 atgtttttga aaataaagtt tctttatctt cgcttcaacc tttgaaaaat atcggttatc
     1441 tttagctaat gctgtatctt tcgcaaattt taaaccgtcc gatttttgac gcggaaggcg
     1501 aaaaaatcgc tgccatacgc gacgttatcg tgcgttacgg cagggaagat tatccgccgc
     1561 tggtcggatt ggtcgcgcgt tatcgccgtc gaaatttttt tatgtcgcgc tcgcgcattt
     1621 ccgctctcgg cgagagcggc gcgaaattgc gga
//
LOCUS       DASWNE010000697         2666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_234878, whole genome shotgun sequence.
ACCESSION   DASWNE010000697 DASWNE010000000
VERSION     DASWNE010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234878"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VGB68_13700"
     CDS             complement(<1..436)
                     /locus_tag="VGB68_13700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEY0050344.1"
                     /translation="MSELSDLYQEVILEHNKNPRNFREIENADKFAEGHNPLCGDQLK
                     LYLMMEDDTVKDVAFKGSGCAISKASASMMTQVIKGKSREEAEILFDEFHKMVLGEMD
                     EEREENRLGKLKIFAGVREFPARVKCASLSWHTLHAALNNETE"
     gene            complement(525..1031)
                     /locus_tag="VGB68_13705"
     CDS             complement(525..1031)
                     /locus_tag="VGB68_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050345.1"
                     /translation="MKRFIRFWSVLSFSLLFSNAAVAQLVSDAYCKDVENSVIKPLNP
                     THWYHNIVFNNECSFEFDIEKNVAVRLIIEKSKTAKAARKSLRSDIEMYEDQHFIDRG
                     KDFKLPGFGRNNFWDEAYFSESYGPMLLRKERTFITVFCDDKKLCPQIEKSLRGTRIL
                     REYRAEQR"
     gene            complement(1036..2280)
                     /locus_tag="VGB68_13710"
     CDS             complement(1036..2280)
                     /locus_tag="VGB68_13710"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020359689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEY0050346.1"
                     /translation="MKTAVKKMNNWDVEKIRKDFPVLSQTVNGKPLAYLDNAASSQVP
                     NLVIERGSKYLQEEHSNVHRGVHYLSQFATTAYEGAREKVKRFINAPEARECIFVRGC
                     TEGVNLVMHGYGRKFVKEGDEIIISAMEHHSNIVPWQMLCEEKGARLRVIPLNRKGEL
                     VLEEYENLLNERTKLVAITHVANSIGTIAPVKEMIATAHKFGVPVLVDGAQAVPHFRV
                     DVQDLDADFYTFSGHKMFAPTGSGVLYGKKELLDKMQPFQGGGSMIRTVSFEKTTYAG
                     IPEKFEAGTPAIASQIGLGAAIDYLNSIDLDAGFAHEHALLEYATEKLSNIEGVKIIG
                     TAAEKASVLSFTVEGVHPHDIGTILDQSGIAVRAGHHCAQPVMKFFEVPATARASFAF
                     YNTREEVDALAEAVEKVIEVFA"
     gene            complement(2280..2609)
                     /locus_tag="VGB68_13715"
     CDS             complement(2280..2609)
                     /locus_tag="VGB68_13715"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEY0050347.1"
                     /translation="MVKRGELYRVLKPTKRDPKNFRVFVVVGRQEVIESSYSTAICAP
                     VYSNCLGIETEVEIGVDEGLKHHSCIRCDELFSLHKSVLTNFVGALSEEKLNELNEAL
                     KIALVIK"
BASE COUNT          643 a          703 c          577 g          743 t
ORIGIN      
        1 cttcagtctc gttattaagc gcggcgtgaa gcgtgtgcca gcttaaagaa gcgcatttca
       61 cgcgcgccgg aaattcgcgc acgccggcaa agattttgag ttttccgagc cgattttctt
      121 cgcgttcctc gtccatttcg cccagaacca ttttgtgaaa ctcgtcgaac aaaatttccg
      181 cctcttcgcg cgatttgcct tttataacct gcgtcatcat cgaagccgac gcttttgaaa
      241 tcgcgcagcc cgaaccttta aaagcgacgt ctttgaccgt gtcgtcttcc atcatcagat
      301 acagcttcaa ctggtcgccg cagagcggat tgtgtccttc ggcgaatttg tccgcgtttt
      361 caatctcgcg gaaatttcgc ggatttttgt tgtgttctaa aattacttcc tgatataaat
      421 cgcttaattc cgacataaaa tattttcgat tgactcaagc aaaatcgtaa aactgttttc
      481 ggcagcttga atcagaattt gccgtagctt ttctacacta aaatttatcg ctgctcagcc
      541 ctgtattctc tcagaattcg agtgccgcgc aacgactttt caatttgcgg gcaaagtttt
      601 ttgtcgtcgc agaacacggt gatgaatgtt ctctcttttc gcagaagcat aggaccgtaa
      661 gattctgaaa aataagcttc atcccaaaaa ttgtttctgc caaatcccgg cagcttaaaa
      721 tcttttcctc tatctataaa atgctggtct tcatacattt caatatccga gcgcaaagat
      781 ttccgcgcgg cttttgcggt tttgcttttc tcgattataa gacgaacggc aacatttttt
      841 tcaatatcga actcaaatga acattcattg ttgaaaacaa tattatgata ccaatgagtc
      901 ggattgagcg gctttataac tgaattttca acatctttgc aataagcgtc tgaaactaac
      961 tgcgcgactg ccgcattcga gaaaagcaag ctaaaactca aaaccgacca aaatctaata
     1021 aaacgcttca taactctatg cgaaaacttc aatcaccttt tcgaccgctt ccgccaaagc
     1081 gtcgacttcc tcgcgcgtgt tgtaaaaagc gaacgaagcg cgcgccgtcg ccggaacttc
     1141 aaaaaatttc atcaccggct gcgcgcagtg atgtccggcg cggacggcga tgcccgactg
     1201 gtcgagaatc gtgccgatgt cgtgcgggtg aacgccttcg acggtgaacg acaaaacgga
     1261 cgctttttcg gcggcggttc cgataatttt gacgccttcg atattcgata atttttccgt
     1321 cgcgtattcc agaagcgcgt gttcatgagc gaaaccggcg tccaaatcaa tcgagttcag
     1381 ataatcgatt gccgcgccca gtccgatttg cgaagcgatg gcgggcgttc cggcttcgaa
     1441 tttttcggga attccggcat acgtcgtttt ctcgaagctg acggtgcgaa tcatcgagcc
     1501 gccgccctga aacggctgca ttttgtccag cagttccttt ttgccgtaaa gcacgccgct
     1561 gcccgtcggc gcaaacattt tatgaccgga aaacgtgtaa aaatccgcgt ccaaatcctg
     1621 cacgtcgacg cgaaaatgcg gaaccgcctg cgcgccgtca accagaacgg gaacgccgaa
     1681 tttatgcgcc gtcgcgatca tttccttaac cggcgcgatt gtgccgattg aattggcgac
     1741 gtgcgttatc gccactaatt tcgtccgttc attgagaaga ttttcgtatt cttccagaac
     1801 cagctcgcct ttccggttca ggggaatgac gcgcaggcgc gcgccctttt cctcgcagag
     1861 catctgccac ggcacgatgt tcgagtgatg ctccatcgcg ctgatgatga tttcgtcgcc
     1921 ttccttgacg aatttgcgcc cgtagccgtg catcaccaga ttgacgccct cggtgcagcc
     1981 gcgcacgaag atgcattctc tggcttccgg cgcgttgata aatcttttca ccttttcgcg
     2041 cgcgccttca taggcggtcg tcgcaaactg cgaaagataa tgcacgccgc gatgcacgtt
     2101 tgaatgctct tcctgcaaat atttcgagcc gcgctcgata accagattcg gcacttgcga
     2161 cgaagccgcg ttgtccagat acgccagcgg cttgccgttt accgtctgcg ataaaacggg
     2221 aaaatctttc cgaatttttt cgacgtccca attgttcatt ttttttactg ccgttttcat
     2281 tatttaatta ccaaagcgat ttttaacgct tcatttaatt catttaattt ttcttcggac
     2341 aatgcgccga caaagtttgt caaaaccgat ttatgcaaac tgaaaagctc gtcgcagcga
     2401 atgcagctat gatgtttcaa gccttcatca actccgattt caacttccgt ttcaataccc
     2461 aaacaatttg aataaaccgg cgcgcaaatc gccgttgaat aacttgattc gatgacttcc
     2521 tgccgcccga caacaacaaa gacgcgaaag tttttcgggt ctcgtttcgt cggttttaaa
     2581 actctgtaaa gttcgccgcg cttcaccatt cagcctgaaa ctccaaagtt tcttcaacct
     2641 gtttgttaat caaatcggcg ttttga
//
LOCUS       DASWNE010000698         1625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_620426, whole genome shotgun sequence.
ACCESSION   DASWNE010000698 DASWNE010000000
VERSION     DASWNE010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620426"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="VGB68_13720"
     CDS             <1..914
                     /locus_tag="VGB68_13720"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HEY0050348.1"
                     /translation="AKSDLNADGTLDYIIVLEKLKKPQEEIYADTSARPTLIIVRDKN
                     EKLKLAARNERVVFCSTCAGGMGDPFDDITIERGGFSITNRGGTRERWIESYDFKYSR
                     RDNNWRLVRVAEINYDSANPEPVRNKAYTPPKHFGKISFADFNPENFKGKGELSESQN
                     KTREVRIYLHRETEIKQLRTPYLVEVRRRVDARAPLAGAIRALLAGGTSEEEKNGMFS
                     FIYGVVLESVQIKNKTARVDFQFDKRTNSRLDPTMEALFHSALEKTATQFPGVKRVLV
                     CVDGVENFFLRKDLHKKCPANREKEKK"
     gene            911..1447
                     /locus_tag="VGB68_13725"
     CDS             911..1447
                     /locus_tag="VGB68_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050349.1"
                     /translation="MKKLILSFLVLAILAANFAPASGQRKRAGRRKNLKTVDVFLRAA
                     GEFDREKYPNELFPVRRRVEAGAPLHNTLEVWTKGANRRDTKLGLASSHFGLEYNCVT
                     LGADGTAIIRFTKPDEVKLPYDEIYGGDIVPPVFIKAAEQTAMQFPNVKKVVVCLDDN
                     LISAEGKSIDSCPGSRLN"
BASE COUNT          424 a          412 c          455 g          334 t
ORIGIN      
        1 atgccaaatc tgatttgaac gccgacggca cgctcgatta catcatcgtc ctggaaaagc
       61 taaaaaagcc gcaggaagaa atctacgccg atacgagcgc gcgcccgacc ttgataatcg
      121 tccgcgacaa aaacgaaaag cttaagctcg ccgcgcgcaa cgagcgagtc gttttctgct
      181 ctacgtgcgc gggcggaatg ggcgacccgt ttgacgacat aacaatcgag cgcggcggtt
      241 tctcgattac caacaggggc ggaacccgcg agcgttggat tgaaagttat gattttaaat
      301 attcgcgccg cgacaataac tggcggctcg tgcgcgtcgc cgaaatcaac tacgactcgg
      361 ctaatccgga accggttcgg aataaggctt acacgccgcc caaacatttc ggcaaaatca
      421 gctttgccga tttcaatccc gagaatttta agggcaaagg cgagctttcc gagtcgcaga
      481 ataaaacgcg cgaggtcaga atttatctgc atcgcgagac cgaaataaaa cagttgagaa
      541 ctccgtacct ggtcgaagta agacggcgcg tcgacgcccg cgcgccgctc gccggagcca
      601 ttcgcgcgct gctcgcgggc gggacttccg aggaagaaaa aaacggtatg ttcagtttta
      661 tctacggcgt cgtgctcgaa tcggtgcaaa taaaaaacaa gacggcgcgc gtggactttc
      721 aattcgacaa gcgaaccaac agccggctcg acccgacaat ggaagcgctt tttcacagtg
      781 cgctggaaaa gacggcgact cagtttcccg gcgtcaagcg cgtcctggtg tgcgtcgacg
      841 gcgtggaaaa tttcttttta cgcaaggatt tgcacaaaaa atgcccggca aaccgggaaa
      901 aggagaaaaa atgaaaaaac tgattttatc atttctggtt ctggcgattc tcgcggctaa
      961 ttttgctccg gcgtcgggac agcggaagcg agccgggcgg cggaaaaact taaaaacggt
     1021 ggacgtcttt ttgcgggcag ccggagagtt cgatagggaa aaatacccga acgagctgtt
     1081 tccggttaga cgccgcgtcg aagcgggcgc tccgctgcac aacacgctcg aagtttggac
     1141 gaaaggcgcg aatcgtcgcg atacgaaatt gggtttggct tcgtcgcatt tcgggctcga
     1201 atacaattgc gtcacgctcg gcgcagacgg cacggcaatt atccgtttta caaagccgga
     1261 tgaggtcaaa ctgccctacg acgaaatcta cggcggagat atcgtcccgc ctgtgtttat
     1321 caaggcggcg gaacagacgg caatgcagtt tccaaacgtg aaaaaggttg tcgtctgtct
     1381 ggacgacaac ctgatttcgg cggagggcaa atccattgat tcctgtccgg gctcgcgcct
     1441 gaattgaaaa ctaaaggaag aaaattatga gaatcgtttt attaatttta atcgcgctcg
     1501 gttcggtgtt tgcgaccttc gggcagattg ctccgaaagg ctggcgcttt cccgaccagt
     1561 gcgacatcaa gcgccggtgg aaggcatacg ttaaggagaa aaagaccgtg ccttatcgca
     1621 tagcg
//
LOCUS       DASWNE010000699         2722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_59258, whole genome shotgun sequence.
ACCESSION   DASWNE010000699 DASWNE010000000
VERSION     DASWNE010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59258"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1917)
                     /locus_tag="VGB68_13730"
     CDS             complement(<1..1917)
                     /locus_tag="VGB68_13730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050350.1"
                     /translation="MLRFARTFCRKGYITALLLNSLFLLSCFTAVRAAGEIDPTFNGG
                     AFTQPGGSLLSDTSNNIILTLPDGKFLVGGKFEAVNGANRNSLVRLNADGTVDSSFNP
                     LSLVGTITSAGLQSNGKIIIAGEFSLTFGAAVTATRLARLNADGTPDPTFTNLTNASN
                     FNGTIADIDVRPDDKILIGGNFSYTAVAGTRINLTRLSANGTDDGAFNLYNLFSSPVF
                     DIAVQPDGKILGFNLPNAGNERFLRRLNEDGTQDASFNVLVNNVIMAVKLQADGKILI
                     GGRFTNVNNVPRQMIARLNSNGTLDDTFASPISSSNEVVINDIEIGIDGMILVGGARF
                     ISGNLNTIVILYRLNNNGGIEPSLNFNGVIPTNGTYTSIVDIAPLPNGQIVIGGEMRR
                     LSVRGAALNRLNADGSVDGSFVTLFGNRALIEDVVVQPNGGIIIGGVFGAINNHVRPS
                     IARFNPDGSADTTFNPTITGANQDPIVHAVAVQPDNKVLVGGQFYGMLVRLNADGSTD
                     FTFATTTLHTSGPVWDIAVQPDGKIIGAGQLVESGGPGGTNTPRFVMRFNQNGTIDQS
                     FLMPPVTSGSQVRKVLVQTDGKVLIGGLFNSIGGNTRSNIARLNADGSLDASFNPLGG
                     ANNIVLDMSLQADGK"
     gene            2178..>2722
                     /locus_tag="VGB68_13735"
     CDS             2178..>2722
                     /locus_tag="VGB68_13735"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY0050351.1"
                     /translation="MKAFSTENIRNLAVIGHGDAGKTQLVSSLLHVAGATTRWGKVDE
                     GTTVTDYDEDSIERKVTLNNNLAHLEYKDTKINLIDTPGYAAFVAHARPALRIADCAL
                     VVVDGVNGIEVQTEKTWVYANEFLLPRFMVINKMDKERADFGHALDTATQSFARSIVP
                     FTLPIGQEANFRGVVDVVHMKA"
BASE COUNT          658 a          728 c          687 g          649 t
ORIGIN      
        1 tttgccgtca gcctgcaagc tcatgtcgag gacaatatta ttcgctccgc ccagagggtt
       61 gaacgacgca tccagacttc cgtcggcgtt caaacgcgcg atattgctgc gcgtattgcc
      121 gccgatgctg ttgaacaagc cgccaatcag gactttcccg tccgtctgca ccagaacttt
      181 tctgacttgt gaccccgatg taaccggcgg catcaaaaag ctttgatcaa ttgtaccgtt
      241 ctggttaaat ctcatcacga agcgcggcgt gttggtaccg cccggaccgc cggattccac
      301 cagttgaccc gcgccgataa ttttcccatc aggctggacg gcgatgtccc agaccggacc
      361 cgatgtgtga agcgtagtcg tggcgaatgt aaagtcggtc gagccgtccg cgttcagccg
      421 cacgagcatt ccgtaaaact gcccgccgac cagcaccttg ttgtccggct gaaccgcgac
      481 cgcgtgcaca atcgggtctt gattcgcacc ggtaatggtc ggattgaaag tagtgtccgc
      541 gctcccgtcc gggttaaatc gcgcgatgct cggacgcaca tgattgttga tcgcgccgaa
      601 aacgccgccg atgataattc cgccattggg ctgcacgacc acgtcttcaa tcaaagccct
      661 gtttccgaaa agcgtgacaa aagagccgtc gaccgagcca tccgcgttca aacgatttaa
      721 cgccgcaccg cgaacggaga gtcttcgcat ctctccgccg ataacgattt gcccgttcgg
      781 caagggcgcg atgtcgacaa tcgaggtgta ggttccgttt gtcggtatca cgccgttgaa
      841 attcagggac ggctcgatgc ctccattatt gttaagcctg tacaaaataa caattgtatt
      901 caagttgccg ctgatgaatc ttgcgccgcc gaccaaaatc atcccgtcga ttccgatttc
      961 gatgtcatta atgaccactt cattcgacga cgaaatcggg ctggcgaaag tatcgtcgag
     1021 cgttccgttc gagttcaaac gggcaatcat ctgacgaggc acattattga cgttggtgaa
     1081 tcttccgccg atgagaattt taccgtccgc ctgaagcttg acagccataa tcacgttatt
     1141 gaccaaaaca ttaaaagagg cgtcctgcgt gccatcctca ttcagacgcc ggagaaatct
     1201 ctcgttgccg gcgttgggca ggttaaagcc gagaatcttc ccgtcgggct ggacggcaat
     1261 gtcgaaaacc ggcgacgaaa acaggttgta taaattaaaa gcgccgtcgt ccgtgccgtt
     1321 cgcgctcaga cgcgtcaaat tgattctcgt tccggcgacg gctgtgtaag aaaaattccc
     1381 gccgatcaga attttatcgt ccggtcgaac gtcgatgtcg gcgatggttc cgttgaaatt
     1441 cgaagcattc gtaaggtttg taaatgtcgg gtccggcgtt ccgtcggcat tcaggcgcgc
     1501 cagacgcgta gctgtgactg ccgcgccgaa ggtcagcgaa aactctccgg cgatgataat
     1561 ctttccgttt gactgcaacc cggcgctcgt gattgttccg acgagcgata ggggattgaa
     1621 agaagaatcg accgtgccgt cggcgttaag ccgaaccaga ctatttctgt ttgcgccgtt
     1681 gacagcctca aactttccgc cgaccagaaa tttcccgtcc ggcagcgtca aaattatatt
     1741 gttcgatgta tcggagagga gagagccgcc cggctgcgtg aacgcgccgc cgttaaaagt
     1801 cgggtcaatc tcgcccgccg cccggactgc cgtaaaacag ctcagtaaaa ataaagaatt
     1861 taacaaaagc gcggttatat aaccttttcg gcaaaaagtt ctggcgaatc gaagcatatt
     1921 tttctcactt tcgtgggcag gtcgatttta attttgtatc agcggtttcg ctcatcagta
     1981 tatgcggttt cgattttcaa gtaaataaat tttaaaacgg aaaataaaaa tccttgtatc
     2041 tggcggatat ttcggctaaa ttaaattctt accagaagtc attccaaaat tttccaaccc
     2101 ttttatgctt gtctaagttt ttggactttc gactttggcg attaaaattt tcaattcaaa
     2161 aggaggtttt gccgaaaatg aaagcatttt ctacagaaaa cattcgcaat ctggctgtca
     2221 ttggacacgg cgacgcgggt aaaactcaac ttgtcagttc ccttttgcac gttgccggag
     2281 caaccacgcg ctggggaaaa gtagatgaag gaaccacagt caccgattac gacgaggatt
     2341 ccatcgagag aaaagtcacg ctcaacaata atctcgccca tctcgaatac aaggacacga
     2401 aaattaattt aatcgacacg cccggttacg ccgcgttcgt cgcgcacgcg cgtcccgccc
     2461 tgcgcatcgc cgactgcgct ctggtcgtcg tcgacggcgt caacggcatc gaggtgcaaa
     2521 ccgaaaaaac ctgggtctac gccaacgaat ttctgctgcc gcgctttatg gtcatcaata
     2581 aaatggacaa ggaacgcgcc gatttcggtc acgcgctcga cacggcgacg cagagctttg
     2641 cgcgctcgat tgttccgttt acgctgccta tcggacagga agcgaatttt cgcggcgtgg
     2701 tcgacgtcgt gcatatgaaa gc
//
LOCUS       DASWNE010000700         5443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_405706, whole genome shotgun sequence.
ACCESSION   DASWNE010000700 DASWNE010000000
VERSION     DASWNE010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5443
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405706"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VGB68_13740"
     CDS             <1..240
                     /locus_tag="VGB68_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050352.1"
                     /translation="RKMEGIIMQAIDVQSWKNVSDTVFVPHTEDEYKRLVEMLDNLID
                     EVGEDETHPLASLMDVLSALIENYENANVPELVLK"
     gene            355..996
                     /locus_tag="VGB68_13745"
     CDS             355..996
                     /locus_tag="VGB68_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050353.1"
                     /translation="MKKLSLVLLLILTSCSIAQNQKPQESINRKFFDSKNACQFLANV
                     GLDQGEFGISGNSEIPNRYMCLGYKFTELKCTSDEELFEIKNYCNRIEYKGYGVKDGV
                     VELKLTYFGGWDDVGHDEDIQIFIKNSDLLISNSLKVKLEDEAKKKILETFDLKTKNQ
                     GEIYRKKYDSAVLSIYRSVMPFGGSFRRKVEFTIYADEYWSDYKNVEREWKVK"
     gene            1018..2130
                     /gene="hppD"
                     /locus_tag="VGB68_13750"
     CDS             1018..2130
                     /gene="hppD"
                     /locus_tag="VGB68_13750"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEY0050354.1"
                     /translation="MTEKNPLGLKKIHHVEFYVGNAKQAEFYYRKAFGFSRAAYSGLE
                     TGNRETTSYVMRQNRVNLVLTTPLNPEHPAAEHIKLHGDGVRDIAFQVEDADQAFHEA
                     VARGARGFIEPHDAADENGSVRRAAIHTYGDTIHSFISYNTGNNGHNYNGAFLPGFVA
                     QEVKGEEVGIVLVDHIVGNVELGKMNFWCDFYRDVMGFERYITFDDKDISTEYSALMS
                     IVMSDGGHNIKFPINEPAEGKGGKSQIQEYIDFYRSAGAQHVALLCKDILQTVENLQA
                     NGVEFLRVPDTYYDELPKRVDCFDENIDDLKRLGILVDSDEEGYLLQIFSKPVEDRPT
                     VFYEILQRKGCKGFGKGNFKALFVSIEEEQRRRGNL"
     gene            2188..3192
                     /locus_tag="VGB68_13755"
     CDS             2188..3192
                     /locus_tag="VGB68_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050355.1"
                     /translation="MPGIKLFLPALILLTSSFTVFSQTEDWKQYSSSLAGLAVKYPSD
                     WQAVESAYERVRKVIFISPGVKDYDVTMNSSIGICSQPKGHVSNSSDSRSRCSRRDDH
                     LSESSKDKVVSEETLQINGLKIRRVVAEDRYRPAATYIYAFFSNKDRDFLVGSYFSRR
                     FNLDRYIPVFDRMLSTVRLLEEKTVLTYRNEKYDFALTYPVSWKSCPIGGNANQDEEE
                     ILNLVPEGRLCNGANYISVSRMTKFSYEKNNRDLKEFLAGKVFSKIVPYLEFGNIHAA
                     LGERADEKYIYRERYFYTNYPQTYELLKISEMCELNNEVCRNQAREILATAQRFLKNR
                     "
     gene            3196..3408
                     /locus_tag="VGB68_13760"
     CDS             3196..3408
                     /locus_tag="VGB68_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050356.1"
                     /translation="MDKAINKYCPRSGERVAADSLTAYKGFVVGFCNPGCRDDFQANV
                     SNRPNDTIYFDAVIKEFELERFAANP"
     gene            3412..3903
                     /locus_tag="VGB68_13765"
     CDS             3412..3903
                     /locus_tag="VGB68_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050357.1"
                     /translation="MDAEVSITFHKGGAKKQDAVVIESARQKHVFKAWRQGIIPHEMV
                     HYAVEKIFGELRGFIRFIGDGIKDADIMEKGGVQALYSEALVNAFQQELFGVLPPEND
                     AFIEYFNDISRRETKFSKAQDFFAYAPDKAKIEATRALLRELTRSWNNTPEGEQIKFS
                     LSL"
     gene            3916..5226
                     /gene="hmgA"
                     /locus_tag="VGB68_13770"
     CDS             3916..5226
                     /gene="hmgA"
                     /locus_tag="VGB68_13770"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEY0050358.1"
                     /translation="MTSPIAGKEELIYQSGFGNEFATEAVKGALPTGQNSPQKAPLGL
                     YAEQFSGTAFTAPRLLNKRTWTYRIRPSVLHKPFRQIENKLLKSSPFGEIVTTPNQLR
                     WNPLPLPERETDFIDGLTTIAGNGDLFSQAGIAIHIYRANRSMDERFFYNADGEMLIV
                     PETGRLGILTELGALRVGAGEIAVIPRGLKFRVALGDEEARGYICENYGAQFRLPDLG
                     PIGANGLANPRDFETPVAWFEDREGEMEIVAKFGGNLWACETDHSPLDVVAWHGNFAP
                     YKYDLLRFNTIGSISFDHPDPSIFTVLTSPSGEAGTANCDFVIFPDRWLVAENTFRPP
                     WYHRNVMSEYMGLIYGQYDAKEEGFVPGGGSLHNQMSAHGPDLDAFEKASNADLQPQK
                     LSGTLAFMFESRYIIRPTRFAMETDALQPEYFEVWQKLKKNFKM"
     gene            5232..>5443
                     /locus_tag="VGB68_13775"
     CDS             5232..>5443
                     /locus_tag="VGB68_13775"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEY0050359.1"
                     /translation="MLPQEKLKYTLDAYIELEKSSEERLEFWDGNIWSMSGGSSVHDQ
                     VQSNAHFALRLKLQGKPCRVFLSDMRV"
BASE COUNT         1480 a         1215 c         1382 g         1366 t
ORIGIN      
        1 aggaaaatgg aaggaataat tatgcaggca attgacgttc aatcctggaa aaatgtatcc
       61 gatacggttt tcgttccgca tacggaagat gaatacaaac gtctggtaga aatgctcgac
      121 aatctcatcg atgaagtcgg cgaagacgaa actcatccgc tcgcctcatt aatggatgtt
      181 ttgtcggcat tgattgaaaa ttacgaaaac gcgaatgtgc cggaattagt tttgaagtag
      241 aaaatttatg ctttactttt cgcgttcaat tttgcttttg atttgtctga tggtttattt
      301 gtgtcttttg tcggggttgt aaaatatagc ttatatttaa ttttcgtaac ccatatgaaa
      361 aaacttagct tagtattact tcttattctt acaagttgta gtattgcgca aaaccaaaaa
      421 cctcaagaat caataaatcg aaagtttttt gattctaaaa atgcttgtca attcttagca
      481 aatgttggtt tagatcaggg ggaatttgga atttcaggaa actcagaaat tccaaacaga
      541 tatatgtgtc ttggttataa atttactgaa ctaaaatgca cctcagatga ggagttattt
      601 gaaataaaaa attattgcaa tcgaattgaa tataaaggct acggtgtcaa agatggagtt
      661 gtcgagttga aacttacata ttttggagga tgggatgatg taggtcacga cgaagatatt
      721 caaattttta ttaaaaatag tgacttactt attagtaatt ccttaaaagt taagttagaa
      781 gatgaggcaa agaaaaaaat attagagact tttgacctaa aaactaaaaa ccaaggagag
      841 atttatagaa aaaagtatga ttctgcggtg ctatccatct atagaagtgt gatgccattc
      901 ggtggtagtt ttcggagaaa ggttgaattt actatttatg ccgatgaata ttggtcagat
      961 tataaaaatg tagagaggga gtggaaggta aaataaaaat attataacga ggcaattatg
     1021 acagaaaaaa atcctttagg tttgaagaaa atacatcacg tcgagtttta cgtcggcaac
     1081 gccaagcaag ccgagtttta ttatcgcaag gcgttcggtt tttcgcgcgc cgcctattcg
     1141 ggcttggaaa ccggcaaccg cgaaacgact tcgtatgtga tgcggcagaa tcgcgtcaat
     1201 ttggttttga cgacgccgct taatccggag catccggcgg cggaacatat aaagctgcac
     1261 ggcgacggcg tgcgcgacat cgcttttcag gtcgaagacg ccgaccaggc ttttcacgaa
     1321 gccgtcgcgc gcggcgctcg cggctttatc gagccgcacg acgcggcgga cgaaaacggc
     1381 tcggtgcgcc gcgccgcgat tcacacatac ggcgacacga ttcactcgtt tatttcctat
     1441 aacacaggca acaacgggca caattacaac ggcgcttttc tgcccggctt cgtcgcgcag
     1501 gaagtaaaag gcgaagaggt cggaatcgtg ctggtcgacc acatcgtcgg caacgtcgag
     1561 ctgggaaaaa tgaatttctg gtgtgatttt taccgcgacg tgatgggttt cgagcgttac
     1621 ataacgtttg acgacaaaga catttcgacc gagtattcgg ctttgatgtc gattgttatg
     1681 tcggacggcg ggcacaacat caaatttccg ataaacgagc cggcggaagg caaaggcggc
     1741 aaaagccaga ttcaggaata catcgacttt taccgttcgg caggcgcgca gcacgtcgcg
     1801 cttttgtgca aggacattct gcaaacggtc gaaaatctac aggcaaacgg cgtcgaattt
     1861 ctgcgcgtgc ccgacactta ttacgacgaa ttgccgaaac gagtcgattg ctttgacgaa
     1921 aacatcgacg atttgaagcg gctcggaatt ctggtcgaca gcgacgagga aggctatctg
     1981 ttgcagattt tcagcaagcc ggtcgaagat agaccgacgg ttttttatga aattctgcaa
     2041 agaaaaggct gcaaaggttt cggcaaaggc aatttcaaag cgctgttcgt ctcgattgag
     2101 gaagagcagc gccggcgcgg aaatttgtaa gcaagaaagc ccgccgccaa tcgttcagac
     2161 ggtgaaattc agaataagga gtttgaaatg cctggaatca aattgttttt gccagccttg
     2221 attcttctga cttcgagctt taccgtcttt tctcaaacgg aagactggaa gcaatacagt
     2281 tcttcgctgg ctggcttggc ggtcaaatat ccttccgact ggcaagccgt agaatcggct
     2341 tacgagcgcg tccggaaggt catttttatc tcgcccggcg tgaaagatta tgacgttacg
     2401 atgaattcct cgataggaat ttgcagccag ccgaaaggac acgtttcaaa ttcttccgac
     2461 agccggagcc gctgcagccg gcgcgacgac catttaagcg aaagctcaaa agacaaagtg
     2521 gtttccgaag agacgcttca gataaacggc ttgaaaatcc gccgagtagt cgccgaagac
     2581 agatacagac ctgcggctac ttatatttac gcgttttttt caaataaaga cagagatttt
     2641 ttagtcggca gttatttttc gaggcgtttt aatttagacc gttacatccc ggtttttgac
     2701 cggatgcttt cgaccgttcg gcttttggaa gagaaaacgg ttttaactta caggaacgaa
     2761 aaatatgatt ttgccctgac ttatccggtt tcgtggaaaa gctgtccgat tggcggaaac
     2821 gcgaatcagg atgaagaaga aattttgaat ctcgttcccg aaggaagatt atgcaacggc
     2881 gcaaactata tttcggtttc gagaatgacg aaattttcct acgaaaagaa caatcgcgat
     2941 ttaaaagagt ttctggcggg caaggttttt tctaaaatcg ttccgtatct ggaatttgga
     3001 aacattcacg ccgctctcgg cgaacgggcg gacgagaaat acatctatcg cgaaaggtat
     3061 ttttacacca attacccgca aacctatgag ctgttgaaaa tctccgagat gtgtgagtta
     3121 aataatgaag tctgccggaa tcaggcgagg gaaattctgg cgacggcgca gagatttctt
     3181 aaaaaccgtt aatatatgga taaagccatc aacaaatatt gcccgcgttc gggcgagcgc
     3241 gtcgccgccg attcgctgac ggcttacaaa ggttttgtcg tcggtttttg caatcccggc
     3301 tgccgcgacg attttcaggc gaacgtgtcg aatcgcccga acgataccat ttatttcgac
     3361 gccgtcatca aagaatttga gctcgaacga tttgccgcaa atccctaaat tatggacgcc
     3421 gaggtctcga taacttttca taaaggcggc gcgaaaaagc aggacgccgt cgttatcgaa
     3481 tccgcccgcc aaaaacacgt tttcaaggct tggcgacagg gaataattcc gcacgagatg
     3541 gttcattacg cggtcgagaa aattttcggc gagctgcgcg gtttcatacg ttttatcggc
     3601 gacggcatca aggacgccga cattatggaa aaaggcggcg ttcaggcttt gtattcggaa
     3661 gcgctggtaa acgcttttca acaggagctt ttcggcgttt tgccgcctga aaatgacgct
     3721 tttatcgaat atttcaacga tatctcgcgc cgcgaaacaa aattttccaa agcgcaggat
     3781 ttttttgcgt acgcgccgga caaagctaaa attgaagcga cgcgcgcgct tttgcgggaa
     3841 ttgacccgaa gctggaacaa tacgcccgaa ggcgagcaga taaaatttag tttatctttg
     3901 taaggaaaat ccgaaatgac atctccgatt gcgggtaagg aagaattaat ttatcaatca
     3961 ggtttcggca atgaatttgc gaccgaagcg gtcaaaggcg cgctgccgac cgggcaaaat
     4021 tcgccgcaga aagcgcctct gggtttatac gccgagcaat tttcgggaac ggctttcacc
     4081 gcgccgcgcc ttttgaacaa aagaacgtgg acgtatcgca ttcgcccgtc ggtgctgcac
     4141 aaaccgtttc gtcaaatcga aaataaactg ctgaaatctt cgccgttcgg cgaaatcgtc
     4201 acgacgccga accagctgcg ctggaatccg ctgccgctgc ccgagcggga aaccgatttt
     4261 atcgacggtt tgacgacgat tgccggaaac ggcgatttgt tttcgcaggc gggaatcgca
     4321 attcacattt accgcgccaa ccgttcgatg gacgagcgat ttttttataa cgccgacggc
     4381 gagatgctta tcgtgccgga gacgggacgg ctcggaattt tgaccgagct cggcgctctg
     4441 cgcgtcggcg cgggcgaaat cgccgtcatt ccgcgcggtt tgaaatttcg cgtcgcgctc
     4501 ggcgatgagg aagcgcgcgg ctacatttgc gaaaattacg gcgcgcagtt tcgcctgccc
     4561 gatttaggtc cgatcggcgc gaacggcttg gcgaatccgc gcgattttga aacgcccgtc
     4621 gcgtggtttg aagaccgcga aggcgaaatg gaaatcgtcg ccaaattcgg cggcaatctc
     4681 tgggcttgcg aaaccgacca ttcgcctctg gacgtcgtcg cgtggcacgg aaacttcgcg
     4741 ccttataaat acgatttgct acgttttaat accatcggct caatcagctt cgaccatcct
     4801 gacccgtcga ttttcacggt tttgacctcg ccttccggcg aagcgggcac ggcaaactgc
     4861 gatttcgtaa tctttcccga ccgctggctg gtcgccgaaa acacgtttcg cccgccgtgg
     4921 tatcaccgca acgtgatgag cgaatatatg ggcttgattt acggtcaata cgacgccaag
     4981 gaagaaggct tcgttccggg cggcggctcg ctgcacaacc agatgtcggc gcacggaccc
     5041 gacctcgacg cctttgaaaa agcctcgaat gccgatttgc agccgcaaaa attgtcggga
     5101 acgttggcgt ttatgttcga gtcgcgctac attattcgac cgacgcgttt tgcgatggaa
     5161 accgacgctt tgcagcccga atattttgaa gtctggcaaa aactgaaaaa gaattttaag
     5221 atgtagtagc tatgttgccg caggaaaaac tcaaatatac tttggacgct tacatcgagc
     5281 tcgaaaaatc aagcgaagaa cgcctggagt tttgggacgg aaacatctgg tcgatgagcg
     5341 gcggcagttc cgttcacgac caggttcaaa gcaacgcgca tttcgccttg cgcctaaaat
     5401 tacagggcaa gccctgccgc gtttttcttt ccgatatgcg cgt
//
LOCUS       DASWNE010000701         2726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_93656, whole genome shotgun sequence.
ACCESSION   DASWNE010000701 DASWNE010000000
VERSION     DASWNE010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93656"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VGB68_13780"
     CDS             complement(<1..436)
                     /locus_tag="VGB68_13780"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002636.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEY0050360.1"
                     /translation="MEHLKQLIREVPDFPKEGINFYDITTLLKDADGLKQTIDALADG
                     FQGQQIDTVIGVESRGFIFAAPLAYHLNAGFVPVRKPKKLPAEKVSVSYELEYGRDTL
                     EMHKDAVGEGHRVLIVDDLLATGGTAKAVTELVEGLGGQIVGL"
     gene            complement(538..813)
                     /locus_tag="VGB68_13785"
     CDS             complement(538..813)
                     /locus_tag="VGB68_13785"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEY0050361.1"
                     /translation="MVVARKFFISGLVQGVGYRFFAQRAAARHQILGYVRNLEDGRVE
                     ALAEGKADAVEGFKHELLAGPTFSKVEHIEELVLEPSKLYSSFRVER"
     gene            complement(922..1737)
                     /locus_tag="VGB68_13790"
     CDS             complement(922..1737)
                     /locus_tag="VGB68_13790"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEY0050362.1"
                     /translation="MTKPEARSPKPDYQIMELVELEKDAPENKPLRLVSDNREPQQFP
                     LIEKQFVNFIFFRVSPDWRKRDAETKTLFKQEFESVFNDFSREFLLFSYSLVGFDSKA
                     DLMFWRIGDSLDLLQDMTAKLYRTNLGGFLETTDNYLSVTKKMMFIPPSESENCEDRY
                     HIKAGEKKYHFVYPCAKNRDWYAKPDEERETMIEENFMVGKKFQNIKIHLTHAFGFSE
                     QEFIISFETDEPKDFLALAEELRQTQANRFTLRGTPIYTCRQRSLTECLDALG"
     gene            complement(1808..2305)
                     /locus_tag="VGB68_13795"
     CDS             complement(1808..2305)
                     /locus_tag="VGB68_13795"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY0050363.1"
                     /translation="MNLGQILAQELQYEAQSTRKMLERVPDKFAWQPHEKSMPLGRLA
                     THVAEIPQWTRAIVTRDELDFAKSDYKPETLNNAIELVELFDKIVADSVELLQNASDA
                     DLMRSWQLRNGEKVLLEMPKAAVVRSMVFNHLIHHRGQLSVYLRLNDVPLPSVYGPTA
                     DEQMF"
BASE COUNT          686 a          665 c          662 g          713 t
ORIGIN      
        1 gcaatccgac gatctgtccg cccaagcctt cgaccagctc ggtgacggcc ttggcggttc
       61 cgccggtggc cagcaaatca tcgacgatca ggacgcggtg cccctcgccg acggcgtcct
      121 tgtgcatttc caatgtgtct cgcccgtatt ccagttcata agacacggaa actttttccg
      181 ccggcagttt cttcggtttg cgaaccggga cgaatccggc gttcagatga taagcgagcg
      241 gcgcggcgaa aataaaaccg cgcgattcga cgccgataac cgtgtcgatt tgctgtccct
      301 gaaagccgtc agccaatgcg tcaatcgtct gctttaaacc gtcggcgtct tttaaaagcg
      361 tcgtaatgtc gtaaaaatta ataccctctt tcggaaaatc cggcacttcg cggataagct
      421 gttttaaatg ttccatactt tgagaattac gaattaaaaa ttacgaatta cgaattgaaa
      481 gaaagattcg ggacgttatc ggaaagtttt cttaattcgt aattcgtaat tgatttttta
      541 cctctcgact ctaaatgatg aataaagctt gctcggttct aaaaccaatt cttcgatgtg
      601 ttcgactttg gaaaatgtcg gaccggctaa caattcgtgt ttaaaacctt cgaccgcatc
      661 ggcttttcct tcggctaaag cttcgacgcg accgtcttcc agattgcgca catagcccag
      721 aatctgatgc cgcgccgcgg cgcgctgcgc gaaaaacctg taaccgacgc cctgcacgag
      781 cccgctgatg aagaatttcc tggctacaac cataattcga ttatgaatta cgaatgacga
      841 atttcaaatt acgaaggacg aattacgaat aaagaaaaca tacttttaat tcgtaattcg
      901 taatttgtaa ttcgtcattg tttatccgag cgcgtcgaga cattcggtca ggcttctctg
      961 ccggcaggtg taaatcggcg ttccgcgcaa tgtgaagcgg ttcgcctgcg tctggcgcaa
     1021 ttcttcggct aaagccagaa aatctttcgg ttcgtccgtc tcaaacgaga tgataaattc
     1081 ctgctcgctg aagccgaagg cgtgcgtcag gtgaatcttt atgttctgaa attttttgcc
     1141 gaccataaag ttttcttcaa tcatcgtctc gcgttcttcg tccggcttgg cgtaccagtc
     1201 gcgatttttc gcgcacgggt agacgaaatg atattttttc tcgcccgctt taatgtggta
     1261 gcggtcttcg cagttttcgc tttccgaagg cggaataaac atcatttttt tggtgacgga
     1321 aagataattg tcggtcgttt ccagaaagcc gcctaaattt gtgcgataaa gctttgccgt
     1381 catatcctga agcaaatcga gcgaatcgcc gatgcgccag aacatcaaat cggctttcga
     1441 atcaaaaccg accagcgaat agctgaacaa taaaaattcg cggctgaaat cgttaaagac
     1501 gctttcaaat tcctgcttga aaagagtttt cgtttccgcg tcgcgcttgc gccaatcggg
     1561 gctgacgcga aaaaaaataa aattgacaaa ctgtttttcg atgagcggaa actgttgagg
     1621 ctcacggttg tccgagacga gccgcagagg cttgttttcg ggcgcgtctt tttccaattc
     1681 gactaattcc ataatttgat aatcgggctt cgggcttcgg gcttcgggct tcgtcaaaga
     1741 atttgctcgc tgtcgagtaa aaaaaatcga agaccgaaga ccgaagacca aagaccgttt
     1801 ccattaatta gaacatctgt tcgtcagccg tcggaccgta gacggacgga agcggcacgt
     1861 cgttgagccg caggtaaacc gaaagctgac cgcgatgatg aatcaggtga ttaaaaacca
     1921 tcgagcggac gaccgccgct ttcggcattt ccagcaaaac tttttcgccg tttctcaatt
     1981 gccagctgcg catcaaatcc gcgtcggagg cgttttgtaa aagctcgacc gaatcggcga
     2041 cgattttgtc gaacaactcg acgagctcaa tcgcgttgtt taaggtctcg ggcttgtaat
     2101 cggatttggc aaagtccagc tcgtcccgtg tgacgattgc gcgcgtccac tgcggaattt
     2161 cggcgacgtg cgttgccagt cgtccgagcg gcatagattt ttcatgcggc tgccaggcga
     2221 atttatcggg aactctttcg agcatctttc gcgtcgactg cgcttcgtat tgaagctcct
     2281 gcgctaaaat ctgacctaaa ttcatatttt ctccgagcct aaattttttg aatttgagag
     2341 agaaatttaa cccgcttatt gtgccaattt ttcagcgcaa tgtccaaacc aaaaagactc
     2401 tttccgaacg gagaaatttt cgattaattt tcaggctggc ttccgacgac ggtttatttt
     2461 ttaaagcgag gcgtaaattt agccgattcg cgccgtgaga atcgaaaaag ggcgacatcg
     2521 cagtcgccct ttgttttcca aattgtccga aatctactaa aacggtaaaa agttgagagt
     2581 cgagaacgga aaattggaga tgttcggaaa gaccagcggc aggtcggtcg ccgataagcc
     2641 gaaccagcgg gacagcgtcg cggcgtaagc ttcgaccgga acggacggaa taaaccgtcc
     2701 gcgcgagtcg gcgtcgtcgg gaccgc
//
LOCUS       DASWNE010000702         6746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_24039, whole genome shotgun sequence.
ACCESSION   DASWNE010000702 DASWNE010000000
VERSION     DASWNE010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6746
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24039"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VGB68_13800"
     CDS             <1..627
                     /locus_tag="VGB68_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050364.1"
                     /translation="LVVERMHLRVPDNPTITAVNGANAINMTVEDVSVETASHPNNLR
                     VPPTNPSVGIYCPKVSNAARVRLKDVNIIGVNTAIKFSEHVVFDDVFIYRVLVALHVE
                     AGGYPNTGKISIEHCKQYMVWVGPSPVNLELSIERAQPGHWWSQDDGTKDLSAPDSRF
                     PHGIIRYIISHQGGGDPYSASSIMTVQNVLPQYLQLQNIFLPTQPQKY"
     gene            complement(897..973)
                     /locus_tag="VGB68_13805"
     tRNA            complement(897..973)
                     /locus_tag="VGB68_13805"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(937..939),aa:Pro,seq:cgg)
     gene            complement(1026..1730)
                     /locus_tag="VGB68_13810"
     CDS             complement(1026..1730)
                     /locus_tag="VGB68_13810"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEY0050365.1"
                     /translation="MEWLTDLLQYLNPKVLIDALLLKLGAFAYVGLFFIVFAETGLAI
                     GFFLPGDSLLVVAGLFAAAGKLDLWVLLITLFIAAVVGDAVGYYSGKKMGKAIFNRPK
                     SRLFNPNHLKKAHAFYEKYGGKTIIIARFVPIVRTFAPIVAGAAEMTYKSFLLFNVFG
                     GFLWVTSMLLAGYFLGGLVEQMMQSWFGIPNFKLENHIDKVVIVIVLLSILPMVIEYL
                     KARREKKREVATGAEV"
     gene            complement(1822..2469)
                     /locus_tag="VGB68_13815"
     CDS             complement(1822..2469)
                     /locus_tag="VGB68_13815"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEY0050366.1"
                     /translation="MIDEYEIPRQRMIQRLREHYQIQDERILNVMRRVPRHFFVPEAL
                     KVQAYADNALPIAGKQTISQPFIVARMTELLELTEQSRVLEIGAGSGYQTVILAQIAR
                     NVYAIERVPQLASEAQARLQQLKIHNVALRCSDGTLGWDVYAPFDAILVAAGSPEIPE
                     PLLQQLKIGGRLVLPVGQDQQTQRLVRVTRAEKGFQTEDFGACAFVPLIGEHGWK"
     gene            complement(2560..3345)
                     /gene="surE"
                     /locus_tag="VGB68_13820"
     CDS             complement(2560..3345)
                     /gene="surE"
                     /locus_tag="VGB68_13820"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEY0050367.1"
                     /translation="MPSEKVRILVTNDDGIHSCGIAELENALREIGDVYVVAPESEMS
                     GASHSLTLARPLRIRQIDERHWTVDGTPTDCVTLALNRILPPQERPHICASGINHGPN
                     LGDDATYSGTVAGAMEAAILGVPGLAFSLIATRNHDFTESAKVARQLTAKAIEEGLPE
                     GTLLNINVPKGVPKGFRITRQGLKNARPIISEHTDPRGKPYYWIGEERSGFHAEGGTD
                     FEAIDEGFVSVTPMRSDLTNYKAIELLKDWNGMKLQIVAGSCG"
     gene            complement(3473..3751)
                     /gene="rpsU"
                     /locus_tag="VGB68_13825"
     CDS             complement(3473..3751)
                     /gene="rpsU"
                     /locus_tag="VGB68_13825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEY0050368.1"
                     /translation="MAFVTVNNNESIESALRRFKRKVMTEEIIKDLKKHAHFVPPGQK
                     AKLKSAMARKRNKKRNRMRPMGNNNNFNNQSSQNQNTGGGFNREPRNG"
     gene            complement(3926..>6746)
                     /locus_tag="VGB68_13830"
     CDS             complement(3926..>6746)
                     /locus_tag="VGB68_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050369.1"
                     /translation="PSEKYVEKMLELVKSDNKVVRNAAVRNLSTLIPQKNPEVIRALL
                     PWLENPKWTKEVGSERRTLIGILDSMVIPESVPGLIAMLNEKETREINVPVVANTNAT
                     YSANSYNITGGMVRQVDSYPYRYSAINALAAQKDARAVPALRLLLSQNEEYERRLVTR
                     AIFLCGGYSVSEQVDALEFIAKNASLRNDEIETNTDTPANVGSAANVVREIDVTDRYV
                     RPNIAVGVNSAVYGATPDLKDARGILGLQIMEHTEPSNELVAGLLERIEILDRKDPKT
                     AAVLRKIIQAWRGAAVNSMLLRDLKNNKLDTDGIVRLLSLRKELREKQFDEISDVRGG
                     SSIAFGISACLTESNSDYDAILASENVESVESKIAMLGCARLIRAPLPVSLVAKHLQS
                     PNKLLALAAERYLESEDSPEARLRVLALYPNEARILGARNAFSYGAATGVNGIFLRDL
                     FISVDEAFTALPFYYFTTDFGELTATEKKLQKEVRENQELLGIYSYDRNYIWIYKDKA
                     VYRREEDASRYRERVLNKQEFDAFKNYLTAERVDEMPPFLSGCEECDARELVMLGRHG
                     GRRIFVRADEPPKFFVELEKMFEEMQKPPSKLHYWLEKNIAGLEILFEDENLQARSVW
                     KNGADFRVLVDNQARRKQIEEELEKQEETEQQQSEEGYDYDKAQQQNAERRAQRQYEN
                     FAWYRFEPAKLSDLIAQPPGVEFIPARDGLPGSVEDRQWKARTANFEIRADEEGLYKI
                     NGGRASKIRAGYYTKPLVTPNGRWAIATKYDDEGPPALVRVNLLTGKELKIKMEQYPT
                     FEAVAFIASVNKALIFGGTYQGEEYQDEESKENRYGNYFLLDVETGLAQPAKSEIRPL
                     AQQTFRPLQPTARPDEFWAAIPDEVKNETQIGIYNARTLAFKSIVKLPQIAFDSMNMW
                     VDEKERKIYFVYQGHLLGLPLPKGS"
BASE COUNT         1542 a         1866 c         1619 g         1719 t
ORIGIN      
        1 ctggtggtcg agcgaatgca cctgcgcgtg cccgataacc cgacgattac ggctgtaaac
       61 ggcgcgaacg ccatcaatat gacggtcgag gacgtcagcg tcgaaaccgc ttcgcacccg
      121 aacaatctaa gggttccgcc gacaaatccg tcggtcggca tctactgccc gaaagtctcg
      181 aacgcggcgc gcgtcaggct caaggacgtg aatataatcg gcgtcaatac ggcgataaaa
      241 ttttccgagc acgttgtttt cgatgatgtt tttatttacc gcgtattagt tgcgctgcac
      301 gtcgaggcgg gcggttatcc gaatacggga aaaatctcca tcgagcattg caaacaatat
      361 atggtctggg tcggaccgtc gccggtcaat ctggaactgt cgattgaacg cgcgcagccc
      421 gggcactggt ggtcgcagga cgacggcaca aaggacttaa gcgcgcccga cagccgtttt
      481 ccgcacggaa tcattcgcta cataatttca catcagggcg gcggcgaccc gtattccgct
      541 tccagtataa tgacggtgca aaacgttttg ccgcaatatc tgcagctgca aaatatattt
      601 ctgccgacac agccccagaa atattaaaat cgcgtaaatc gtgaacggca tttatcgttc
      661 agcataaaaa ttggcggcgt atgatattaa agcgaaaaga ttaatcaaca tttcacgatt
      721 cacgacttgc gattcgcgct ctgcccgccg gcgcgcacgc tttatccaac ccgattttta
      781 ttgcacgaaa ctgcttttgc aaaaaaattt catcaaactt ttttacaaca tcccgaccga
      841 aaaaagaaaa ggctttccgg attcgttcgg aaagcctttt cttttttttt aataaatggt
      901 cggggcgaga tgatttgaac atccgacctc acggtcccga accgtgcgct ctaccaggct
      961 gagccacgcc ccgataagtc aagttttaaa gtttaattca ccgccgcccc gaagtcaaac
     1021 ggcaattaga cttccgcccc ggtcgctact tcccgctttt tctcgcgccg tgctttcagg
     1081 tattcgataa ccatcggcag aatcgaaagc aaaacgatga cgatgacgac tttgtcgata
     1141 tgattttcga gcttgaaatt cgggattcca aaccagctct gcatcatctg ctcgaccaga
     1201 ccgccgagaa aatagcccgc cagaagcata ctcgtgaccc acaaaaaccc gccgaaaacg
     1261 ttaaagagga gaaagctttt ataagtcatt tccgccgcgc ccgcgacaat cggcgcaaag
     1321 gtgcggacaa tcgggacgaa gcgggcgatg ataatcgttt tgccgccgta tttttcataa
     1381 aacgcgtgcg cttttttcag atgattcgga ttgaacagac gcgatttcgg gcgattaaaa
     1441 atcgctttgc ccatcttttt tcccgaataa taaccgaccg catcgccgac caccgccgca
     1501 ataaacagcg ttatcagcaa aacccacaaa tcgagcttgc ccgccgccgc gaaaagtccg
     1561 gcgacgacca gcaaggaatc gcccggcaga aaaaaaccga ttgccagtcc ggtttcggca
     1621 aagacaatga aaaataatcc gacataggca aacgcgccca gcttgagcag cagcgcgtcg
     1681 attaaaactt tcggattaag atattgcagt aaatccgtca accattccat aagtcttata
     1741 atgcaaagtg cataatgcaa aattaaagaa aaaatttaaa tttgaatcct gaattccaaa
     1801 tcctgatatt ttgcatttaa ttcacttcca cccgtgctcg ccaatcagcg gcacaaacgc
     1861 gcacgcgccg aaatcttctg tctgaaagcc tttttccgcg cgcgtaacgc gaacgaggcg
     1921 ctgcgtctgc tggtcttgtc cgacgggcaa aacgagcctg ccgccgattt ttaactgctg
     1981 cagcagcggt tcgggaattt cggggctgcc cgccgcgacc agaatcgcgt caaacggcgc
     2041 gtagacgtcc cagccgagcg ttccgtccga gcagcgcaga gcgacattgt gaattttcaa
     2101 ttgctgaagt cttgcctgcg cctcgctcgc cagctgcgga acgcgctcga tcgcgtagac
     2161 gttccgggca atctgcgcca gaatcaccgt ttgataaccg gagcccgcgc cgatttccag
     2221 cactctcgac tgctccgtca gttccagcag ctcggtcatt cgcgcgacga taaacggctg
     2281 tgaaatcgtc tgttttcccg caatcggcag cgcgttgtcg gcgtatgcct gcacttttaa
     2341 ggcttcggga acaaaaaaat gacgcggcac gcggcgcatc acatttaaaa ttcgctcgtc
     2401 ctgaatctgg taatgttcgc gcaaacgctg aatcattcgc tgacgcggaa tttcgtattc
     2461 gtcaatcata agagttgaca ggaagttgac aggaagttgc ggatagttgc aggtagttat
     2521 taaagattag aactctggaa ctgttttaac tatccgcaac tatccgcaac taccggcaac
     2581 tatctgcaac ttcatcccgt tccaatcttt caataattcg atggctttgt aattcgtcaa
     2641 atcgctgcgc atcggcgtga cggaaacgaa gccttcgtca atggcttcaa aatccgtgcc
     2701 gccctcggcg tgaaaaccgc ttcgctcttc gccaatccaa taatacggtt ttccgcgcgg
     2761 gtcagtgtgc tcggaaataa tcggtcgagc gtttttcagc ccttgcctgg taatgcgaaa
     2821 acctttcgga acgcctttcg gcacattgat gttcagcaga gttccttcag gcaaaccttc
     2881 ctcgattgct ttggcggtca actgccgcgc aacttttgcc gactcggtaa aatcgtgatt
     2941 gcgcgtcgct atcaggctga aagccagacc gggaacgcct aaaatcgcgg cttccatcgc
     3001 gccggcgacc gttcccgaat aggtcgcgtc gtcgcctaga ttaggaccgt gattgatgcc
     3061 cgaagcgcaa atgtgcggtc gctcttgcgg cggcagaatg cggttcagcg caagcgtcac
     3121 gcaatcggtg ggcgtgccgt caaccgtcca atggcgttcg tcgatttgtc tgattctgag
     3181 cggtcgggct aaagtcaggc tgtgagatgc gcccgacatt tccgattcgg gcgcgacaac
     3241 gtacacatcg ccgatttcgc gtaaagcgtt ttcaagttcg gcaattccgc acgaatggat
     3301 gccgtcgtca ttcgtcacca gaatccgaac cttttccgaa ggcatagaaa aatttcgatt
     3361 ttcgattgcc gatttgcgac ttcgcaggaa attaattttt gattttcggt ttatccggct
     3421 cgcgccgtat ttcgtcttca atcaaaaatc gcaaatctaa aatctaaaat ctttagccgt
     3481 ttctcggttc gcggttgaag ccgccgccgg tattctggtt ctggctgctc tggttattaa
     3541 aattattatt gttgcccatc ggacgcatgc ggttgcgctt tttgttgcgc ttgcgagcca
     3601 tcgccgattt cagtttcgct ttctgtccgg gcggcacgaa atgcgcgtgt tttttcaaat
     3661 ctttaataat ctcttcggtc atcactttgc gcttgaaacg gcgcagcgcg ctttcgattg
     3721 actcgttatt atttactgtt acaaaagcca ttttaaaatt tccatctcct taatctccga
     3781 cacagtcggc tcgtctgcca tcaggcgaaa tgattattaa acactttttt caccgcaaag
     3841 gcaagttttg agatgaattc cggattcaaa attcaggatt ccgaatttga aatcccgaat
     3901 cctgaatttt gaattaagtt ttgcgttagc tgcctttcgg cagcggcaag ccgagcagat
     3961 gtccctggta aacgaaatag attttccgct ctttctcgtc aacccacata ttcatactgt
     4021 cgaaagcgat ttgcggcagt ttgacaatgg atttaaaggc gagcgtcctg gcgttataaa
     4081 tgccaatctg cgtttcgttt ttcacttcgt cgggaatcgc cgcccagaat tcgtccggtc
     4141 tggcggtcgg ttgaagcggg cgaaaagttt gctgcgcgag cgggcgaatc tcgctttttg
     4201 ccggctgcgc caatccggtt tcgacgtcga gcagaaaata attgccgtat ctgttctcct
     4261 tgctttcttc gtcctgatac tcttcgccct gataagtgcc gccgaaaatg agcgccttat
     4321 taaccgacgc aataaaagcg acggcttcaa aggtcggata ttgctccatt ttaattttga
     4381 gttccttgcc cgtcagcaaa ttaacgcgca ccagcgcggg cggtccttcg tcgtcgtatt
     4441 tggtggcaat cgcccagcgc ccgtttggcg taaccagcgg cttcgtataa tatccggcgc
     4501 ggattttact cgcccgaccg ccgttgattt tatacaaacc ttcctcgtcc gcccggattt
     4561 caaaattcgc cgttcgggct ttccattgcc ggtcttcgac gcttccgggc aagccgtcgc
     4621 gcgcgggtat gaattcgacg cccggcggct gagcaatcaa atccgaaagc tttgccggct
     4681 cgaatctata ccacgcgaaa ttttcatact gccgctgcgc tcgccgctcc gcgttctgct
     4741 gctgcgcttt gtcgtaatcg tagccttctt cgctttgctg ctgctcggtc tcttcctgtt
     4801 tttccagttc ctcttcgatt tgtttgcggc gcgcctgatt atcgaccagg actcgaaaat
     4861 cagcgccgtt tttccagacc gaacgcgcct gtaaattttc gtcctcgaac aaaatctcca
     4921 agcccgcgat gtttttttcc agccagtagt gaagtttgga gggcggcttt tgcatttctt
     4981 caaacatctt ttcaagctcg acgaaaaact tcggcggctc gtcggcgcgc acgaaaatcc
     5041 gccgtccgcc gtggcgaccg agcatcacta attccctggc gtcacactcc tcgcagccgg
     5101 acaaaaacgg cggcatctcg tcgactctct ccgccgtcag atagtttttg aaagcgtcga
     5161 attcctgttt gttcaaaacg cgctcgcggt agcgcgaagc atcttcttcc cttctgtaaa
     5221 ctgctttatc cttgtaaatc catatataat ttctgtcgta ggaataaatt cccaaaagct
     5281 cctgattttc cctgacctct ttttgcagtt ttttctcggt cgccgtcagc tctccaaaat
     5341 cggtggtgaa ataataaaac ggcagcgccg taaaagcttc atcgacgctg ataaacaaat
     5401 cccgcaaaaa aataccgttg acgccggtcg ccgcgccgta agaaaaagca tttcgcgcgc
     5461 cgagaattct cgcttcgttc ggatagagcg ccagaaccct cagtcttgcc tccggcgagt
     5521 cctcgctttc cagatagcgt tccgccgcca gcgcgagcag tttattcggg ctttgcagat
     5581 gtttggcgac caggctgacg ggcagcggcg cacgaatcaa gcgggcgcag ccgagcatcg
     5641 caattttgct ctcgacgctt tcgacgtttt cgctcgccag aatcgcgtca tagtcgctgt
     5701 ttgattcagt cagacaagcc gaaatcccga acgcaatcga gcttccgccg cgaacgtcgg
     5761 aaatttcgtc aaactgtttt tcccgcagct cttttcgcag gctcaaaagc ctgacgatgc
     5821 cgtcggtatc gagcttattg tttttcaaat cccggagcag catcgaattt accgccgcgc
     5881 cgcgccaggc ttggataatt tttcgcaaaa cggcggcggt tttcgggtct ttccggtcga
     5941 gaatctcgat gcgttcgagc aaaccggcga ccagttcatt gctcggctcg gtatgctcca
     6001 ttatctgaag tcctaaaatc cctctcgcgt ccttcaggtc gggtgtcgcg ccgtaaaccg
     6061 ccgaattgac gccgaccgcg atatttgggc gcacatacct atcggtcaca tcgatttccc
     6121 ggacgacgtt agctgccgag ccgacgttgg cgggcgtatc tgtgttcgtc tctatctcgt
     6181 cattgcgcag gctggcgttt ttggcgataa actcaagcgc gtcaacctgt tcggaaacgg
     6241 aatagccgcc gcataaaaat atagcccggg tgaccagacg gcgctcgtat tcctcgttct
     6301 gcgacagtag aaggcgcaac gccggaaccg cccgcgcatc tttctgcgcc gccagagcat
     6361 ttatcgccga gtagcggtaa ggatatgaat caacctgtct gaccattccg ccggtgatgt
     6421 tataagaatt agccgaatac gtcgcgttcg tattggcgac gaccggaaca ttaatttcac
     6481 gggtttcctt ttcattgagc atcgcaatca agcccggaac gctttcggga atcaccatcg
     6541 aatccagaat tccaatcaat gtccgccgct cgctgccgac ttcctttgtc catttcggat
     6601 tttccagcca cggcagcaga gcccttataa cttcgggatt tttctgcggg ataagcgtgc
     6661 tcaggttgcg caccgccgcg ttgcgcacga ctttgttgtc gcttttgacc agttcgagca
     6721 ttttctcgac gtatttttcc gaaggc
//
LOCUS       DASWNE010000703         2153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_306798, whole genome shotgun sequence.
ACCESSION   DASWNE010000703 DASWNE010000000
VERSION     DASWNE010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306798"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..1408)
                     /locus_tag="VGB68_13835"
     CDS             complement(233..1408)
                     /locus_tag="VGB68_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY0050370.1"
                     /translation="MKFSRRDFLRASALFLTGVAVSPKQVFSFESEDRDLQMLVLGDS
                     VMWGQGLLEKDKFWFLTKQWLENKTNRKVAAHVEAHSGATILWRKDQKYPKDRVTTFN
                     GELNLSTPTIMQQVDNAVKHYGNNSIKREDVDLVLVNGGANDIGIANLLNPSRNQSWM
                     QRKTAEILGEDMPKLLEKIYISFPQARVVVTGYYPAVSSETSTEHICNRLKAVSGIDR
                     YEKIINRFLKFLGVKNRRFDACATNFIKERVDRLVKTLSALSDIWQAESNRYLQQAVN
                     NLNEKYPLSPASGDDQRRAFFVEAPFGSKNAYAAPETYLWQVVLSEVPGNYKSNDVFY
                     QLREQVCGCKRIRLEGFSLTKCFIAGTGHPNIAGARKYADAIEQEFGEVLPLTGWGN"
     gene            complement(1536..1955)
                     /locus_tag="VGB68_13840"
     CDS             complement(1536..1955)
                     /locus_tag="VGB68_13840"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HEY0050371.1"
                     /translation="MSENIINSSRAPEPVGLYPHARRVGNLLFLSGVGPRERGRREIP
                     GVELDENGNIISYDIEAQCRSVFQNVRYILEESGSAWEKIIDVTVFLTNMKDDFQIYN
                     RVYAEYFKDNLPCRTTVEINKLPTPIAIELKVIAAIE"
BASE COUNT          538 a          523 c          489 g          603 t
ORIGIN      
        1 cgtcgtaagc gcattgattg ccgacgggcg gtttcagcag gtgccgatgt tcttcaatcc
       61 attgtttgaa attaaaaggt cttctgatag acataaattt taacctcgat tttcgctgtg
      121 catttccgat tttttacgaa atcgctcgat aaataaattg taatcccgaa ccgccttaaa
      181 agagaatatt ccgaagtaag acagtttatt tttccgcttt ttcggaaagc ttttaattac
      241 cccagccggt taaaggcaaa acttcgccga attcctgctc gatagcgtcg gcgtattttc
      301 ttgcgccggc aatattcgga tgtccggttc cggcgataaa acacttcgtc aaactaaaac
      361 cttcgagccg gatgcgcttg cagccgcaaa cctgctcgcg cagttgatag aaaacgtcgt
      421 tcgatttgta atttcccgga acctcgctca gaaccacttg ccacaggtag gtttcgggcg
      481 cggcgtaggc gtttttcgag ccgaaaggag cctcgacgaa aaacgcccgc cgttggtcat
      541 cgcccgacgc cgggctgagc gggtattttt cgtttaaatt atttaccgcc tgttgcagat
      601 atctgtttga ttccgcctgc cagatgtcgg acagagcgct gagcgtttta accagccggt
      661 cgactcgctc tttgataaaa tttgtcgcgc aggcatcaaa acgcctgttc ttaacgccta
      721 aaaatttgag aaatctgttg ataatttttt cataccggtc gattcccgaa acggctttta
      781 gacgattgca aatgtgctcg gtcgaggttt cgctggaaac cgccggataa taacccgtga
      841 cgacgacacg cgcctgcgga aacgagatgt aaatcttttc cagcagtttc ggcatatctt
      901 cgcccaaaat ctcggcggtt tttctctgca tccagctttg atttcgcgaa gggttcagca
      961 aattggcgat tccgatatcg ttggctccgc cgttgaccag caccaaatcg acatcttcgc
     1021 gcttgatgga attgtttccg taatgtttaa ccgcgttatc gacctgctgc ataatggtcg
     1081 gcgtcgaaag atttaattcg ccgttaaaag tcgtcacacg gtctttcgga tatttctggt
     1141 ctttacgcca gaggatggtc gcgccggaat gcgcttcgac gtgtgccgcg acttttcgat
     1201 tggttttatt ttccagccat tgtttcgtca gaaaccagaa tttatctttt tcgagcaagc
     1261 cctgtcccca catcaccgaa tcgccgagga cgagcatctg caaatcacga tcttctgatt
     1321 cgaaagaaaa gacttgtttc ggactgaccg cgacgccggt taaaaataat gccgaagctc
     1381 tgagaaagtc ccggcggctg aatttcataa taaggcgatt ctccttctaa aaactccgga
     1441 aaaggtgaaa actatattac acaagaattt gcgcggataa cagtgtaaac gattgtattc
     1501 atttgtattt agattattca cccggcgaag cgaagttatt cgattgcggc gatgactttc
     1561 agctcaatcg caatcggcgt cggaagtttg ttaatctcaa ccgtcgtgcg acacggcaga
     1621 ttgtccttaa aatattcggc gtaaacgcga ttgtaaatct gaaaatcatc tttcatattg
     1681 gtcagaaaaa ccgtcacgtc aatgattttt tcccacgccg agccggattc ttccagaata
     1741 tagcgcacat tctgaaaaac cgaccggcat tgcgcctcga tgtcgtaaga tatgatgttt
     1801 ccgttttcgt ccaattcgac gccgggaatt tctcgcctgc cgcgctcgcg cggtccgacg
     1861 ccggacagaa acaaaagatt tccgacgcga cgcgcgtgcg ggtataaacc gaccggctcc
     1921 ggcgctctgg aagaattaat aatgttttcg ctcatagctg aataattcag gattcaggat
     1981 tcaggattca ggattgaaga tttaagattg gagatttagg atttgccgtt atccaaagac
     2041 caatcccgaa tcctgaatcc tgaatcttga atcttaaatc ctgaatcttg aatcttgaat
     2101 cctgaattct taatccgttt tccgctcgcc gttgtaaacc gcgtaagcgc att
//
LOCUS       DASWNE010000704         1577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_620579, whole genome shotgun sequence.
ACCESSION   DASWNE010000704 DASWNE010000000
VERSION     DASWNE010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620579"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="VGB68_13845"
     CDS             complement(<1..981)
                     /locus_tag="VGB68_13845"
                     /inference="COORDINATES: protein motif:HMM:NF039826.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HEY0050372.1"
                     /translation="MFGLMRVKKCGMSEAEKNFRRLHYCGTCKTIGSLYGQKSRLLLN
                     HDAVFLAEILTALDNENAADWQSAYQSYNCLKLPKNEMPLPLEFAATANLILAEFKLA
                     DKITDENSRAGKIAQKTFSNSFQKAQGKLEEWKFPFEIVREILSAQEKIEIVSLRSNK
                     IPDEILNELSSPTAETTAIFFGEGVRLMGKSELENPVAEIGFRFGKLIYLLDAFEDYE
                     KDFRRKQFNAIRAAFRVDEEKISSEIKRKIVSILQKLESEIVDGIYKLPIAENQKALF
                     ASRLRENLQRKLKTSLPVLKTKKTCQPSRKQSFSERWKNASAAARKMASGF"
     gene            complement(990..1514)
                     /locus_tag="VGB68_13850"
     CDS             complement(990..1514)
                     /locus_tag="VGB68_13850"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HEY0050373.1"
                     /translation="MEEIKNQPRGVLTKEKRREIELEESLESLSRYLDDWVKIPVVGW
                     RFGLDALIGLVPNVGDMATSLLSFYILIAGVRYGVPKITLLRMAFNIGLDYVVGAIPF
                     VGDAFDFFWKSNKQNMDLIRERATGKDKGTTGDYLFVLATILILIAILIGSILVSLYI
                     LGTVFRGIWDSVNF"
BASE COUNT          398 a          407 c          341 g          431 t
ORIGIN      
        1 aaacccgctt gccatttttc gcgccgctgc cgacgcgttt ttccatcttt ccgaaaaact
       61 ttgtttacgg ctcggctggc aagttttttt cgttttcaaa acgggcagag acgttttcag
      121 ttttctttgc agattttccc gcaggcgcga ggcgaaaagc gctttttgat tttccgcaat
      181 cggtaattta taaattccgt cgacgatttc gctttccagt ttctgcaaaa tcgaaacgat
      241 ttttcgcttg atttcagacg aaattttttc ttcatcgaca cgaaacgcgg cgcgaatcgc
      301 gttaaattgt tttcgccgaa aatctttctc gtaatcttca aacgcgtcga gcaggtaaat
      361 caattttccg aagcgaaaac cgatttccgc aaccgggttt tcaagctcgc ttttgcccat
      421 caacctcacg ccttcgccga aaaaaatcgc ggtcgtttcg gcggtcgggc tggaaagctc
      481 atttaaaatt tcgtccggaa tcttgttcga gcgcagggaa acaatctcga ttttttcctg
      541 agcggataaa atttctcgaa caatttcaaa cggaaatttc cattcctcaa gtttcccttg
      601 cgccttttga aaactgttcg agaaagtttt ttgcgcgatt ttgcccgcgc gtgaattttc
      661 atccgtgatt ttgtcggcga gcttaaattc ggcgagaatc agattagcgg ttgccgcgaa
      721 ttcgagcggc agcggcattt cgtttttcgg cagcttcaga cagttgtagg actgataggc
      781 gctctgccag tccgccgcat tttcattgtc gagagcggtc aaaatctcag ccagaaaaac
      841 cgcgtcgtga ttaagcagaa gccgcgattt ttgcccgtaa agactgccga ttgtcttgca
      901 cgttccgcaa taatgaagcc gccgaaagtt tttctccgcc tcggacattc cgcatttttt
      961 gacccgcatc aagccgaaca tttttcacct cagaaattga cagaatccca aattcccctg
     1021 aaaacagttc ccaaaatata caagcttact aaaatcgaac caatcagaat ggcgattaaa
     1081 atcaggattg tcgccagcac gaaaagataa tcgccggtcg tgcctttgtc ttttcccgtc
     1141 gcgcgttcgc gaatcaaatc catattctgc ttgttcgatt tccagaaaaa atcgaacgcg
     1201 tcgccgacga acggaatcgc gccgaccaca taatcgagcc cgatattgaa agccattctg
     1261 agaagcgtga ttttcggaac gccatagcgc acgccggcga tgagaatata aaacgaaaga
     1321 agcgaggtcg ccatatcgcc gacgttcggc accaagccga tcagcgcgtc gagcccgaaa
     1381 cgccagccga cgaccggaat tttcacccag tcgtcgagat aacgcgacag gctttccagg
     1441 ctttcttcca gctcgatttc ccgccgcttc tcttttgtca ggacgccgcg cggttgattt
     1501 tttatttctt ccatattttt aactattcgg aatacaggaa aaatgaaacc acagatttac
     1561 acggatgaac aacgata
//
LOCUS       DASWNE010000705         3981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_163172, whole genome shotgun sequence.
ACCESSION   DASWNE010000705 DASWNE010000000
VERSION     DASWNE010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163172"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..141)
                     /locus_tag="VGB68_13855"
                     /pseudo
     CDS             complement(1..141)
                     /locus_tag="VGB68_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002784177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(247..753)
                     /locus_tag="VGB68_13860"
     CDS             complement(247..753)
                     /locus_tag="VGB68_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050374.1"
                     /translation="METLELDEDEVKTMTFDLKRELLRHLVAALTFRARIAVAGAPEN
                     FAAFKIDETVRTPAEILAHLGDLLQGSLYLMKGELVYLNSRPLAWKEESARFFSAAEK
                     LDSYLASDAPLNQPVEKITQGPIADALTHVGQIVMLRRAAGSPVRVESYFTVEIVPAE
                     IHTESFAR"
     gene            974..1630
                     /locus_tag="VGB68_13865"
     CDS             974..1630
                     /locus_tag="VGB68_13865"
                     /inference="COORDINATES: protein motif:HMM:NF018626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HEY0050375.1"
                     /translation="MNSFFRFFLSALLFLNFACESNSQFIKRRTAPASASIAPKVVEV
                     ESAEIQKMLESAFEQTSLTKAYDGAYVEIPYPNGDVPMETGVCTDVVIRAFRRAGTDL
                     QKEVHEDMRANFDFYPKKWGLPKPDTNIDHRRVPNLQTFFARRKKALEITRNAADYKP
                     GDVVSWDIDGKGMTHIGLVSNLWNEATKRFLIIHNIGGGAKAEDALFNWKITGHYRYF
                     "
     gene            complement(1737..2201)
                     /locus_tag="VGB68_13870"
     CDS             complement(1737..2201)
                     /locus_tag="VGB68_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050376.1"
                     /translation="MQNIELQNIRPQIRSYIKETNPAEQIAGFFYYSSGAILWTAGSL
                     GISAFLIGLLFSPGMTLGMSILGLIFGFPGWVWAGIGVFLFRGHKNYYQFGLKKDEAV
                     LLWVLTIAYNGAQAAYIFTYLNDTFAGNIIGGWNLIVAALAALALYFELKKY"
     gene            complement(2445..2861)
                     /locus_tag="VGB68_13875"
     CDS             complement(2445..2861)
                     /locus_tag="VGB68_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050377.1"
                     /translation="MPANEQDVINLIKAGKLDASELEQMFSSDASLSGLVTVTDWGIC
                     YSSATGQLSEFATVTSNSSSNPITGIGLIAYSSNSSKMFCVQYTNGFSSNSIATSIGT
                     TLYNPQTDGSQAACVVYGWTNNGSFYVPQTMTIGSC"
     gene            complement(3115..>3981)
                     /locus_tag="VGB68_13880"
     CDS             complement(3115..>3981)
                     /locus_tag="VGB68_13880"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0050378.1"
                     /translation="DVDGLHLHYQQFGERSAPVLLLIHGYTASTAVWRAVAPLLAERG
                     FQVIALDLVGFGYSSKPAWFDYSIQSQARVVSRFMNRLGVGKATVVGSSYGGAVASTL
                     ALDYPERVEKLVLVDAVCNDDAKRHPVLRLANLPGMGEIISAFLSDSPRFVRYRLKNT
                     LAPANHHLITDDRIQTIRRPLRARNAHHSVLMTARNWSACRIEQDAQFISQPTLLVWG
                     ENDTVIPIQNGYKLHDAILNSRLVVFKDCGHNPQEEKSENFVEVVAEFAADKKGGIAA
                     RENEPMRTDGNG"
BASE COUNT          993 a         1012 c          958 g         1018 t
ORIGIN      
        1 ttctctgacc tcttccgcat agccttgccg cttcggctct ggcgaaaaag ttttgccgca
       61 gtttcggcat aaataccgct gtgtgccgca gcgactcttt ccgtttttta tttgtttctc
      121 tgaattttca cagtttggac acgctggcat taataaactt tatcctatca acattcgttt
      181 agtcactccc tttctcgcgt gcagcatccc gccttgagcc ggttgaaagc gaataatctt
      241 ttttgtttaa cgcgcaaagc tctccgtgtg aatttcagcg ggaacgattt caactgtaaa
      301 ataactttca acccggacgg gcgaaccggc ggcgcggcgc agcatcacga tttgtccgac
      361 gtgcgtcagg gcgtcggcaa tcggaccctg cgtgattttc tctaccggtt gattaagcgg
      421 agcatccgaa gccaggtagg aatcgagttt ttcggcggcg gagaaaaacc tcgccgattc
      481 ctctttccac gcaagcggac gggaatttaa ataaaccaac tcgcctttca ttaagtagag
      541 cgagccttgc agtaaatcgc cgagatgcgc caaaatttcc gccggagtcc tgacggtttc
      601 atcaatttta aaggcggcga aattttccgg cgcgcccgcg acggctatcc tcgcgcgaaa
      661 agtcagagcg gcgaccagat gtcgcaataa ttcacgcttc aaatcaaatg tcattgtttt
      721 tacctcgtct tcgtcaagct ctaatgtttc catcccgtta tttttcgggt tgccgagaat
      781 acttgtaaaa acggctcctg tcaagcattt atatgtttgc acagcaaact ttcgccgaaa
      841 tgccggaaac gccttttatc gacggaacaa ttattgttta taaaacgttt attcttttcg
      901 ggaaaaagct tgcttttttc ctgaacgcat ctaaaatatt cggcggaaat ctttcgcttt
      961 aaaggatttt gtaatgaata gttttttccg gtttttcctg tccgctcttc tgtttcttaa
     1021 tttcgcctgc gagtcgaatt cacagtttat caaacgccgg acggctccgg cgtcggcgag
     1081 cattgcgccg aaggtcgtcg aggttgaatc cgccgaaatc caaaagatgc tcgaaagcgc
     1141 gtttgagcaa accagcctga cgaaagccta cgacggggcg tatgtcgaga ttccgtatcc
     1201 gaacggcgac gtgccgatgg aaaccggcgt ctgcacggat gtcgtgattc gcgcgtttcg
     1261 acgcgccgga acggatttgc agaaagaagt tcacgaagat atgcgggcaa acttcgattt
     1321 ttacccgaaa aaatggggct tgccgaaacc cgatacaaac atcgaccacc ggagagttcc
     1381 gaatctgcaa accttctttg cgcggcggaa aaaagcgctg gaaataacgc gcaatgccgc
     1441 cgattataaa cccggcgacg tcgtcagctg ggatatagac ggcaagggaa tgacgcatat
     1501 cggcttggtt tcaaatctct ggaacgaggc gaccaaaaga tttttaatca ttcacaatat
     1561 cggcggcggg gcgaaagccg aagacgctct attcaattgg aaaatcaccg ggcattatcg
     1621 gtatttctga tttttaaacc agttcattaa cgccgttgtc gacaacaaaa ccgctgccga
     1681 ccgcgagaat atcggcattt cgtaaaattg tcccggtttt cgtaattcgg gagtcttcag
     1741 tattttttca gctcgaaata aagcgccagc gccgcaagcg cggcgacaat caagttccaa
     1801 ccgccgatga tgtttccggc gaaagtatca tttaaataag tgaaaatgta cgccgcctga
     1861 gcgccgttat acgcaatcgt taaaacccaa agcaaaacgg cttcgtcttt tttcaagccg
     1921 aattggtaat aattcttatg accgcgaaac aaaaagacgc cgattccagc ccaaacccag
     1981 cccggaaaac cgaaaatcaa tcccagaatc gacatcccga gcgtcattcc gggcgaaaat
     2041 aaaagtccaa tcagaaatgc cgatatgccg agcgagcccg ccgtccagag aatcgcgccg
     2101 gacgaataat agaagaatcc ggcaatctgc tcggctggat ttgtctcttt aatataagac
     2161 ctgatttgcg gtcggatgtt ttgtaattcg atgttttgca ttttgccctc gctaatcgga
     2221 agtgttttga ctccgacagg cgagagagca aagctcgtgc cgcttgagaa aaataagaaa
     2281 aacctcgcat ttaccgtgct tttacaaaat ccggttttgc aaagtgccgc cgcgaggctt
     2341 tgattttcgc aaattgaaaa gaaatgtaaa aaggcaaaag gtaaaaaata agtcaacttc
     2401 ttacttttac cttttacctt ttgcctttta tctttcgcct ttttttagca cgagccgatg
     2461 gtcatcgtct gcggcacata aaagcttccg ttattcgtcc agccgtagac gacgcacgcc
     2521 gcctgactgc cgtcggtctg cggattgtag agcgtcgtgc cgatggaagt cgcaatcgaa
     2581 ttgctgctaa aaccgtttgt gtactgcacg caaaacattt ttgagctgtt gctcgaataa
     2641 gcgatcagcc cgatgccggt tatcggattg ctcgaactgt ttgaagtgac cgtcgcaaat
     2701 tcgctcaact gcccggtcgc cgaactataa catattcccc aatcggtaac cgtgaccaga
     2761 ccgcttagcg aagcatctga ggaaaacatc tgttcaagct cggacgcgtc gagttttccc
     2821 gctttgatta aattaatgac atcctgttca tttgcaggca taatatttgc tccttttttt
     2881 attttttaat gagttgaaga atatcttcaa gagcaacata ctcctatctt gtttgctttg
     2941 caatttcaaa tttaatattt atttatagcc gggcgataaa tgtttcggaa tttaacgagc
     3001 ctcgactttt ctcagctcaa agccgtcgaa ttcaaaagcg agcgacccgc tttccggaag
     3061 atattcgcgg ctgatttccg tcgttgacaa taaataaatt aaatctgccg gtaattaacc
     3121 gtttccgtcc gtccgcatcg gctcgttttc gcgggcggcg attccgcctt ttttatctgc
     3181 ggcaaattcg gcgacgacct cgacaaaatt ttccgacttt tcttcctgcg gattgtgacc
     3241 gcaatctttg aaaacgacga gccgcgagtt gagaatcgcg tcgtgcagtt tgtaaccgtt
     3301 ttgaatcggg ataaccgtgt cgttttcgcc ccagacgagc agcgtcggct ggctgataaa
     3361 ctgcgcgtcc tgctcgatgc ggcaggcgct ccagttgcgc gcggtcatca gaaccgaatg
     3421 gtgcgcgttt ctggcgcgga gcggtcgccg aatcgtctgg attctgtcgt ccgttatcaa
     3481 atgatgattg gcgggcgcga gcgtgttttt caggcgatag cggacaaagc gcggcgaatc
     3541 gctcaaaaat gccgagatta tttcgcccat tccgggcaga tttgccagcc gcagaaccgg
     3601 atgtcttttc gcgtcgtcgt tgcagacggc atcgacgaga accagttttt cgactcgttc
     3661 cgggtaatcg agcgcgagcg tcgaagcgac cgcgccgccg taagagctgc cgacgacggt
     3721 cgccttgccg acgcccagac gattcataaa acgcgaaacg acgcgcgcct gcgattgaat
     3781 cgaataatcg aaccacgccg gcttgctcga atatccgaaa ccgaccaaat caagggcgat
     3841 aacctgaaag ccgcgctcgg caagcaatgg cgcgaccgct cgccagacgg cggtcgaagc
     3901 cgtgtagccg tgaatcaaaa gcagaaccgg agcgctgcgc tcgccgaatt gctggtaatg
     3961 caggtgcaag ccgtcaacat c
//
LOCUS       DASWNE010000706         2637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_516183, whole genome shotgun sequence.
ACCESSION   DASWNE010000706 DASWNE010000000
VERSION     DASWNE010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516183"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="VGB68_13885"
     CDS             complement(<1..847)
                     /locus_tag="VGB68_13885"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEY0050379.1"
                     /translation="MNSSSNAIFQPSKSPLITFRIQFLTGSIDDPEGKEGLASLTAAM
                     LAQGGSKNLTYEQIVEQFYPMATGFGWQVDKEMTTFTGQTHADNLPKYYALIRQMLLE
                     PGFREEDFSRLKDEAINFFKVNLREGNDEELGKERLYNIIYAAHPYEHHSRGKVSRLE
                     KLTIQDVKDFYAENYTRANLVTGLSGGFDAKFSRQLTIDFQKLPKGARLMRKIGQPNL
                     ENGLKIDIVKRETRATAISLGLPIPVNRTSGKDYVALALVASYFGQHRSSNSYLYGRI
                     RELRGL"
     gene            complement(985..2331)
                     /locus_tag="VGB68_13890"
     CDS             complement(985..2331)
                     /locus_tag="VGB68_13890"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0050380.1"
                     /translation="MKLYIILIALLCLASVINSSAQARRVFPYQYKTDDLPNGLRVVT
                     VPTDYPNLVALYIVVGAGSRNEVEARKSGYAHFFEHLMFRGSENFPAGKFDEVMKKSG
                     ASSNAYTSDDRTVYHETFAKEDLDEVMRLEADRFQRLKFSEEQYKTEAGAVLGEYNKN
                     SANPTSKMHEVLRGTAFTNHTYSHTTMGYLEDIKDYPNQYEYAWQFFNRFYRPENTTI
                     IVVGDVKQPEVLAMVKKYWSDWKPGDYKQQIPVEKPQDKARTKHIEWTSQTLPHVVVS
                     YRAPAFSETEKDKAALDLLSAVAFGENSELYQKLVLQEQKAVWISPDFDNRIDAELFS
                     VWSQVKDEKDLNYVRDEIIKTYQEFTRELIPQKQLDETRSRLRYSFSMAMNSNDAIAG
                     TLAQYIALRRSPDTIDKVFALYDSITPEDIRFYAEKYFKPNSQTIITLATKKGGAK"
BASE COUNT          588 a          658 c          701 g          690 t
ORIGIN      
        1 taagcccgcg cagctcgcgg atgcgcccgt aaagatagct gttcgacgag cgatgctgac
       61 cgaaatacga agcgaccagc gcgagggcga cgtaatcttt gccgctcgtg cggttgaccg
      121 gaatcggcag accgagcgaa atcgccgtcg cgcgcgtttc gcgtttgacg atgtcgattt
      181 ttaagccgtt ttccagatta ggctgcccga tttttcgcat caggcgcgcg cctttcggca
      241 gtttttgaaa atcaatcgtc aattgtctgg aaaatttcgc gtcgaaaccg cccgaaagac
      301 ccgtgacgag atttgcccgc gtgtaatttt ccgcataaaa atccttaacg tcctgaatcg
      361 tcagtttttc cagtctcgaa acttttccgc gcgaatgatg ctcgtaagga tgcgcggcgt
      421 agatgatgtt gtaaagcctt tccttgccga gttcctcgtc gttgccttcg cgcagattga
      481 ccttgaaaaa attgattgct tcgtctttca gacgcgaaaa atcttcttcg cgaaaaccgg
      541 gttcgagcag catctggcga atcagcgcgt aatatttcgg cagattatcg gcgtgggtct
      601 gcccggtaaa ggtcgtcatt tccttgtcga cctgccagcc gaagccggtc gccatcgggt
      661 agaactgctc gacaatctgc tcgtaagtca gatttttcga gccgccctgc gcgagcatcg
      721 cggcggtcag cgaagccaag ccttccttgc cttcggggtc gtcaatcgag ccggtcaaaa
      781 attgaatgcg aaacgtgatg agcggcgatt tgctcggctg gaaaatggcg tttgagcttg
      841 aattcatagg ttttgacgcg ggttttgtgc tgccgcgctt ctgagcgaaa atcgaaaatg
      901 cgaagacggc gattaaagcc gcggagaagc aaagacgcag agaatcacac gttaaatttt
      961 gcagtttgcg ctttaaattt tgcattattt cgcgcctcct ttcttcgtcg ccagcgtgat
     1021 aatcgtctgg ctgttcggct tgaaatattt ttccgcgtag aagcgtatgt cttccggcgt
     1081 gatggaatca tatagcgcaa agactttatc aatcgtgtcg ggcgagcggc gcagagcgat
     1141 gtattgcgcc agcgtgccgg cgatcgcgtc gttgctgttc atcgccatcg aaaagctgta
     1201 gcgcaggcgc gaacgggttt cgtcgagctg tttctgcggt atcaattccc tggtgaattc
     1261 ctgataagtt ttaatgattt cgtcgcgcac gtaattcaaa tccttctcgt ctttgacctg
     1321 cgaccagacg gagaaaagct cggcgtcgat gcgattgtcg aagtccggcg aaatccagac
     1381 ggctttctgt tcctgcaaaa ccagcttttg ataaagctcg gaattttcgc cgaaggcgac
     1441 ggcgctcagt aaatccagcg ccgctttgtc tttttccgtt tcggaaaaag cgggcgcgcg
     1501 atagctgacg acgacgtgcg gcaaagtctg cgaagtccat tcgatgtgtt tggtgcgcgc
     1561 tttgtcctgc ggcttttcga ccggaatctg ctgcttgtaa tcgcccggtt tccaatcgct
     1621 ccagtatttt ttcaccattg ccagaacttc cggctgcttt acgtcgccga cgacgataat
     1681 cgtcgtgttt tcaggacgat aaaagcgatt gaaaaactgc cacgcgtatt cgtattgatt
     1741 cgggtaatcc ttgatgtctt cgagatagcc catcgtcgtg tggctgtatg tgtgattggt
     1801 aaaagcggtt ccgcgcagca cttcgtgcat ttttgaagtc ggattggcgg agtttttgtt
     1861 gtattcgccc aaaaccgcgc ccgcctcggt tttgtattgt tcctcgctga atttcaaacg
     1921 ctggaaacgg tcggcttcga gccgcattac ctcgtccaaa tcttctttag cgaaagtttc
     1981 gtgataaacc gtccggtcgt cagaggtata cgcgttgctg gacgcgccgg attttttcat
     2041 tacttcgtca aactttcccg ccggaaaatt ttcgctgccg cgaaacatca aatgctcgaa
     2101 aaaatgcgcg taaccgcttt ttctggcttc gacttcgttg cgcgagccag cgccgacgac
     2161 gatatacagc gcgaccagat tcggataatc ggtcggaacg gtcacgacgc gcaagccgtt
     2221 cggcaaatcg tcggtcttat attgatacgg aaaaaccctg cgcgcctgcg ccgaagaatt
     2281 aatgacggac gccagacaaa gcaaggcaat taaaattata taaagtttca tcttgacctc
     2341 tgtagaagag aatttttcat ttcgttagat gcagaaattt tactgaaaaa aacacatttc
     2401 cgctatagcg atttcacgga tattaaaact tcgcggcgaa aaactttact aacccggctc
     2461 tttgatattt actggaataa aggatttgcg gttcagcgcg atggaaacgg ttgacgaaaa
     2521 aatcacgaac gccgaggagc aggcgcaggc gcgagtttcg gcgtggcggc tcggcggctt
     2581 gtcgtggcgg acgctcgggc ggcgcgtctg gaacgagctt tacgccggcg cgctact
//
LOCUS       DASWNE010000707         1938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_306838, whole genome shotgun sequence.
ACCESSION   DASWNE010000707 DASWNE010000000
VERSION     DASWNE010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306838"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VGB68_13895"
     CDS             complement(<1..434)
                     /locus_tag="VGB68_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050381.1"
                     /translation="MHFAFCISSCSVPNLESPECSAARTAVREFYSNHFGGDMKFTPE
                     NLKAREKHLTPDLARLLQKFLTDSDPFTLSAPDDLPKAFRAGGCKVAAPDKTELQILL
                     FWKDDVRSEQREITVEAVKQNETWLINNISSNTSNLQNLLNH"
     gene            complement(488..769)
                     /gene="gatC"
                     /locus_tag="VGB68_13900"
     CDS             complement(488..769)
                     /gene="gatC"
                     /locus_tag="VGB68_13900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEY0050382.1"
                     /translation="MDVRKVAKLAHLEITDEEVALYTPQMDSIVAYVEQLNELDTENI
                     EPATGGLTAEGAATEADREDVPHESLGQKLALEQAPDAHSGHFRVPKVL"
     gene            complement(838..1311)
                     /locus_tag="VGB68_13905"
     CDS             complement(838..1311)
                     /locus_tag="VGB68_13905"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEY0050383.1"
                     /translation="MNELFRALPALLRQMEDNESLRQAVVFAAWRRIAGEGLREHAVA
                     LRLYQKHLIVAVMSETWKKHLEHLSGQMIFKLNSTLEQPLVTFIEFRVDEETIRQERE
                     KYQKKAIDEETQRQIALEQVTPKMRHAADAIKDENLRYQFLLAAGSCLARKKNME"
     gene            1382..>1938
                     /locus_tag="VGB68_13910"
     CDS             1382..>1938
                     /locus_tag="VGB68_13910"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEY0050384.1"
                     /translation="MIKLPRLILASGSPRRAEILTAAGWQFTKQIADIDESPGEAETP
                     ENYVRRLAREKAVAVAALHENALVLGADTTVVINDEIIGKPFDLDDARRMLKKLSGNR
                     HEVLTGVALVKVFTPPGYSAPQFETEVAFERTLVKFAAMNDAEVEFLVESGEPLDKAG
                     AYAVQAQAALFIEGIEGDYWNVVGLP"
BASE COUNT          425 a          498 c          507 g          508 t
ORIGIN      
        1 taatggttta aaagattttg taaattgctc gtattactgg aaatattgtt tatcagccag
       61 gtttcgtttt gtttgacggc ttcgacggtt atctcgcgct gctcgctccg cacgtcgtcc
      121 ttccagaaaa gcaaaatctg caattcggtt ttgtccggcg cggcgacttt gcagccgccg
      181 gcgcgaaacg ctttcggtaa atcgtccggc gcggaaagcg taaacgggtc tgaatcggtc
      241 agaaactttt gcagaagcct cgctaaatcc ggcgtcagat gtttttcccg cgccttcaga
      301 ttttcgggcg tgaatttcat atcgccgccg aaatgattcg agtaaaactc tctgaccgca
      361 gtccgcgccg cgctgcattc gggcgattcc agattcggaa cgctgcacga agaaatgcaa
      421 aacgcaaaat gcaaaacgca aaatataaac agccaatttt gaatttggaa tttggaattc
      481 tgcattatta aagaactttc ggcacgcgga aatgtcccga atgcgcgtcg ggcgcttgtt
      541 cgagcgcgag cttttgtccc agcgattcgt gcgggacatc ttcgcggtcg gcttcggtcg
      601 ccgcgccttc cgccgtcaat ccgccggttg ccggttcgat gttttccgtg tcgagctcgt
      661 ttaactgctc gacgtaggcg acgatgctgt ccatctgcgg cgtgtaaagc gctacttctt
      721 cgtctgtaat ttccaggtgc gccagcttgg ctacttttct aacgtccata aattaatgct
      781 cggtgctcaa ttgtcagtca cggattttgc cgtcgacggc tgcctttgac agctagctta
      841 ttccatattc ttttttcgcg ccagacagct tccggcggcg agcagaaact ggtagcgcag
      901 attttcgtcc ttgattgcgt cggcggcgtg acgcattttc ggcgttacct gttcgagcgc
      961 gatttgtcgc tgtgtttcct cgtcaatcgc ttttttctgg tatttctcgc gctcctgcct
     1021 gatggtctct tcgtctaccc gaaattctat aaaagtgacg agcggttgtt caagcgtcga
     1081 atttaattta aaaatcatct gcccgctcag atgttccaga tgttttttcc acgtctcgct
     1141 catcacggcg acaatcaggt gtttttgata aagccggagc gcgacggcgt gttcgcgcaa
     1201 gccttcgccc gcaatcctgc gccaggcggc gaaaacgacc gcctgacgca agctttcatt
     1261 atcttccatt tgccggagca gcgcgggcaa tgcccggaac agttcattca ttggtcatta
     1321 aagatttatc atttatcatt taaacaaata caaatgttaa atgttaaatg gtaaatgttc
     1381 aatgataaaa cttcccagat tgattttagc ttcgggcagc ccgcgccgcg ccgaaatttt
     1441 aacggctgcc ggctggcagt ttaccaaaca aatcgccgac attgacgaat ctcccggaga
     1501 agcggaaacg ccggaaaatt acgttcggcg tctggcgcgg gagaaagccg tcgcggttgc
     1561 cgcgcttcac gaaaacgcgc tggttttagg agccgacacg accgtcgtga ttaacgacga
     1621 gattatcgga aagccgttcg atttggacga cgcgcgccgg atgctcaaaa aactttcggg
     1681 aaaccggcac gaagttttga cgggcgtcgc gctggtgaaa gtgtttacgc cgcccggcta
     1741 ttcggctccg caattcgaaa ccgaggtcgc ttttgaaaga acgctcgtca aatttgccgc
     1801 gatgaacgat gcggaagtcg agtttttagt cgaaagcggc gagccgctcg acaaagccgg
     1861 ggcgtatgcc gtgcaggcgc aagccgcgct gtttatcgaa ggaatcgaag gcgattactg
     1921 gaacgtggtc ggtttgcc
//
LOCUS       DASWNE010000708         1597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_163268, whole genome shotgun sequence.
ACCESSION   DASWNE010000708 DASWNE010000000
VERSION     DASWNE010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163268"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1207
                     /locus_tag="VGB68_13915"
     CDS             <1..1207
                     /locus_tag="VGB68_13915"
                     /inference="COORDINATES: protein motif:HMM:NF012611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050385.1"
                     /translation="GASIMPGGRREVYEHVRPIFEAVAARADGEPCVAYMGNSSAGHF
                     VKMVHNGIEYGLMQILAETYDFMLRVLKMNYSRMSRTFSEWNDSELNSFLVEITAEVL
                     RKVDDESGKPLVEMILDKAAQKGTGKWTSQAAMDFGVPIPTIDSAVAMRQISAQKEAR
                     VLIAEKFGSRLPQLKPHSPEDGQIVSSRLVTSPKAARAEHEVETVVSEHHKQTIGEQA
                     AKTEGQAIGNHADEQKNAAMREEFAEAFPEFVEQLKNALLSSFIVTYAQGMSLLQTAS
                     KEKNYNLNLSEIAKIWRGGCIIRSALLEEMRRAFAENPELPNLILDDKFAEILNKNRE
                     DWRSITAKFTESRISALCLSSALAYFDAFRSERLPANLIQAQRDFFGAHTYQRIDKEG
                     IFHTPDWNE"
     gene            1330..>1597
                     /locus_tag="VGB68_13920"
     CDS             1330..>1597
                     /locus_tag="VGB68_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050386.1"
                     /translation="MDFNKKNRHRRRSREESDKAQGSHKLLIVTLALTVFFTALGFYF
                     FKGKFSGAENKQYATKQEIQQVVDNLKNKLNYLSISIPDDRRRVS"
BASE COUNT          440 a          392 c          406 g          359 t
ORIGIN      
        1 cggcgcgagc attatgccgg gcggcaggcg cgaagtttac gaacacgtcc gaccgatttt
       61 tgaagccgtc gccgcgcgcg ccgacggcga accgtgcgtc gcctatatgg gaaattcgag
      121 cgccggtcat ttcgtcaaaa tggttcacaa cggcatcgaa tacggcttga tgcagattct
      181 ggcggaaact tacgatttta tgctccgcgt tttgaagatg aattacagcc ggatgagccg
      241 gactttttcc gaatggaacg attcggagct gaattctttt ctggtcgaaa tcaccgctga
      301 ggttttgcgc aaagtcgatg acgaaagcgg aaagccgctg gtcgagatga ttctcgataa
      361 agccgcgcag aaaggaaccg gcaaatggac ttcgcaggcg gcgatggatt tcggcgtgcc
      421 gattccgacg attgattcag ccgtcgcgat gcgccagatt tcggcgcaga aagaagcgcg
      481 cgttttgatt gccgaaaaat tcggcagtcg cctgccgcag ctcaagccgc attcgccgga
      541 agacggtcag attgtttcga gccgtctcgt caccagcccg aaagccgccc gcgccgaaca
      601 cgaagttgaa accgtcgtca gcgagcatca caagcaaact atcggcgagc aggcggcgaa
      661 aactgaaggg caagcaatcg gaaatcacgc cgacgagcaa aaaaatgcgg ctatgcgcga
      721 agaatttgcg gaagcttttc ccgaattcgt cgagcaattg aaaaacgctc tcctgagctc
      781 gtttatcgtc acctacgcgc agggaatgtc gcttttgcag acggcttcaa aagaaaaaaa
      841 ttacaatctg aatttgtcgg aaatcgccaa aatctggcgc ggcggctgca ttattcgctc
      901 ggctctgctc gaagaaatgc gccgcgcttt cgccgaaaat ccggagctgc cgaacctgat
      961 tctcgacgac aaattcgccg aaattttaaa taaaaaccgc gaagactggc gctcgattac
     1021 cgccaaattt acggaaagcc gcatttccgc gctctgccta tcgtcggcgc tggcttattt
     1081 cgacgctttc cgctccgagc gtcttcccgc caatctgatt caggcgcagc gcgacttttt
     1141 cggcgcgcac acttaccaga gaatcgacaa agaaggaatt tttcacacgc cggactggaa
     1201 cgaataatgt attaaattcg ttattctatt acgcagaaga aaaataagag gaaattaatt
     1261 cagcacgatt cagattcaag ctaatcgcgc cgatataatg tccgattctc aataaaagga
     1321 gtaaatagaa tggattttaa taaaaaaaat cggcatcgca ggcgttcgag agaggaaagt
     1381 gataaagccc aaggaagtca taagctattg attgtgacgc tcgctttaac agtatttttc
     1441 acggcgctcg gtttttattt cttcaaaggt aaatttagcg gcgctgagaa caaacaatat
     1501 gctacgaagc aggaaatcca gcaggtcgta gataacctga aaaacaaact caattatttg
     1561 tcaatttcaa ttcctgatga ccggcgcagg gtttctt
//
LOCUS       DASWNE010000709         1642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_693664, whole genome shotgun sequence.
ACCESSION   DASWNE010000709 DASWNE010000000
VERSION     DASWNE010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693664"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..1060
                     /locus_tag="VGB68_13925"
     CDS             68..1060
                     /locus_tag="VGB68_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEY0050387.1"
                     /translation="MNLFPFADYWWFYGIFLLFVLLMLALDLGVFNRKAHVVSFKEAT
                     IWSIVWVALALVFNFIFYQYAASKFGAETGKQAALEFLTGYVIEYSLSIDNIFVFVLV
                     FSYFGIPPVYKHRVLFYGILGALVFRALFIALGSALMQFHWVIYLFGGFLIITGIKMM
                     FSNQEEIEPEKNFLIRIFKRFMPVTHEIDGKNFFVRKDGILHATPLFIALLFLEATDI
                     IFAVDSVPAIFAVTREPLIVFTSNIFAILGLRSMYFMLAGAITKFHLLKYGLAVVLVF
                     VGLKMVWLNDFYGGKFPISWSLLFILGTIALSVVVSLVFPLKPENAPDELTKVQ"
     gene            complement(1172..>1642)
                     /locus_tag="VGB68_13930"
     CDS             complement(1172..>1642)
                     /locus_tag="VGB68_13930"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0050388.1"
                     /translation="GENEMRKYWLPWLVGMPAETSRAICSLIFSGTLEKCPNLRICFA
                     HGGGSFPATIGRIEHGFLARPDLCAADNQCNPRDYLGKIYFDSLVHDAAMLDYLVKLA
                     SAEKVALGTDYPFPLGEREAGKLIEDGNFDQRTKALLLHGAALKWLDLNKEDFL"
BASE COUNT          354 a          403 c          396 g          489 t
ORIGIN      
        1 tcagaaaagt cgtgaaaggt ctttcctttt gcgctgaaat aataaatatt tccgaggagt
       61 tttttttatg aatctgtttc cctttgccga ctactggtgg ttttacggca tatttttatt
      121 attcgtttta ttgatgctcg cgctcgattt gggcgttttc aaccgcaagg cgcacgtcgt
      181 ttcgtttaag gaagcgacca tttggagcat cgtctgggtc gcgctcgcgc tcgtctttaa
      241 cttcattttt tatcaatacg ccgcgtcgaa attcggcgcg gaaacgggca agcaggcggc
      301 gctcgaattt ctgaccggct acgtcatcga atattcgctt tcaatcgata acattttcgt
      361 tttcgttctg gtcttcagct atttcgggat tccgcccgta tacaagcacc gcgttttgtt
      421 ttacggcatt ttgggcgcat tggtctttcg cgctctgttt atcgcgctcg gctcggcgct
      481 gatgcagttt cactgggtca tctatctttt cggcggcttt ctgattatca ccggaatcaa
      541 gatgatgttt tcgaatcagg aagaaatcga gccggaaaag aattttctga ttcgcatttt
      601 caaacgattt atgccggtca cccacgagat tgacggcaaa aatttctttg tccgcaaaga
      661 cggaattctg cacgcgacgc cgcttttcat cgcgcttttg tttctggaag cgaccgatat
      721 tatcttcgcg gtcgacagcg ttccggcgat tttcgccgtc acccgcgagc ctttaatcgt
      781 ctttacgtcg aatattttcg cgattctcgg acttcgctcg atgtatttta tgctggcggg
      841 cgcgattacg aaatttcatt tattgaaata cggactggcg gttgtgctgg tattcgtcgg
      901 cttgaaaatg gtctggctca acgattttta cggcggcaaa ttcccgatta gctggtcgct
      961 tctgtttatt ctcggcacga ttgccctttc ggtcgtcgtt tcgctcgttt ttccgctgaa
     1021 accggaaaac gctcccgatg aattaactaa agtccagtaa atataaaacc ttcggaatag
     1081 atgaaagatg ataattgata gattttatct ttcatctatc atctattccg agtcgtatta
     1141 cataacgcaa aatccgcgct gaaatctccg tttataaaaa atcttcttta ttcaaatcga
     1201 gccatttcag agccgcgccg tgcagcagga gcgcttttgt ccgttggtcg aaattgccgt
     1261 cttcaatcaa tttccccgct tcccgttcgc cgagcggaaa cgggtaatcg gtgccgagcg
     1321 cgaccttttc ggcgctcgcc agtttgacca gataatcgag catcgccgca tcgtggacga
     1381 gcgaatcgaa ataaattttg ccgagataat cgcgcgggtt gcattgattg tcggcggcgc
     1441 ataaatccgg acgcgcgaga aaaccgtgtt ctatccgccc gatggtcgcc ggaaacgagc
     1501 cgccgccgtg ggcgaagcaa atccgcagat tcgggcattt ttcgagcgtg ccggaaaaaa
     1561 tcaaagagca aatcgcgcgc gaggtttcgg cgggcattcc gaccagccag ggcagccagt
     1621 attttcgcat ctcgttttcg cc
//
LOCUS       DASWNE010000710         1569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_306893, whole genome shotgun sequence.
ACCESSION   DASWNE010000710 DASWNE010000000
VERSION     DASWNE010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306893"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1569
                     /locus_tag="VGB68_13935"
     CDS             <1..>1569
                     /locus_tag="VGB68_13935"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HEY0050389.1"
                     /translation="VVTDRRKWKYLAGDDLDWASAELDDSSWDELENSSINRQAPPPS
                     GWNGRGWFRLHFQVDEFMANKPVALEARQFGASEVYLNGRLVTKFGEIREQGEIEYNP
                     AYIPVPVEFRAGENVLAVRYSASVLAENDTLRGRWLRNGKVRPGFSFQFDDIEDFKTT
                     IQTYSDVVAGRFAVFYIGVLTALGLLHLLMFLFYRIERANLFYSIHAFAFAAHLVCIN
                     NFAYGHWGTIQNLIFASMNTLLVGALFVALLAFLHVAFERKFGKMFWLLAAAWTAAIA
                     VNVVFLRAFVWLPALTSVTTLLTFIFCIYLLVRALRQKRPGAWILMGGVILFTTALSI
                     TLLTVNGILPLSYNYYFYLEIFIVLSIPVAVSIFLALNFARTSQNLSERLAEVKQLSD
                     RQIEQERREAELRAENERRTRELEEARQLQISMLPKKLPELPNLEIAAYMKPATEVGG
                     DYYDFHVSEDGTLTVAVGDATGHGLKAGSVVTATKSLFNAFAGESDIARIFQKTSAAL
                     KKMNLRGLFMAMAML"
BASE COUNT          336 a          408 c          430 g          395 t
ORIGIN      
        1 cgtcgtaact gaccggcgca aatggaaata tctggcgggc gatgatttag actgggcgtc
       61 ggctgagctg gatgattcga gttgggacga gctggaaaat tcgagcataa accggcaggc
      121 tccgccgccg agcggctgga acgggcgcgg ctggtttcgg cttcattttc aagttgacga
      181 atttatggca aacaaacccg tcgcgcttga agccagacaa ttcggcgcat cggaagttta
      241 tttaaacggg cggctcgtca cgaaattcgg cgaaatcagg gaacagggcg aaattgaata
      301 caatccggct tacattccgg ttccggtcga gtttcgcgcg ggcgaaaacg tcctggcggt
      361 cagatattcg gcgtcggttt tggcggaaaa cgacacgttg cgcggcaggt ggctgcgaaa
      421 cggcaaggtc agaccgggtt tttcttttca gttcgacgac atcgaggatt ttaaaaccac
      481 gattcaaact tacagcgatg tcgtcgccgg gcgcttcgcc gttttttata tcggcgtgct
      541 gacggcgctc gggctgctgc atctattgat gtttttgttt taccgaatcg agcgcgccaa
      601 cttgttttac agcattcacg ctttcgcttt cgccgctcat ctggtttgca ttaataattt
      661 tgcttacggg cattggggaa cgattcagaa tctcatcttc gcttcgatga acacgctttt
      721 ggtcggcgcg ctgtttgtcg cgctgctcgc ttttttgcac gtcgcattcg agcgcaaatt
      781 cggcaaaatg ttctggctgc tcgccgcggc gtggacagcg gcaatcgcgg taaacgtcgt
      841 ttttctgcgc gctttcgtct ggctgcccgc gctgacgagc gtgacgaccc tcttaacttt
      901 tattttctgc atttatctgc tcgtccgcgc gctccggcaa aagcgccccg gggcgtggat
      961 tctgatgggc ggcgtcatac tctttacgac cgcgctcagc atcactttgc tgaccgtaaa
     1021 tggaattttg cctttatctt ataactatta tttttatctt gaaattttca tcgtgctgtc
     1081 gattccggtg gctgtttcaa tttttctcgc gctcaatttc gctcgcacca gccagaattt
     1141 aagcgagcgt ctagccgaag tcaaacaact ttcagacaga caaatcgagc aggaacgacg
     1201 cgaggcggag ctgcgcgccg aaaacgaacg ccgaacccgc gagcttgaag aagccagaca
     1261 gctgcaaata tcgatgttgc ctaaaaagct gcccgaactg ccgaatctgg aaatcgcggc
     1321 gtatatgaaa ccggcgacgg aagtcggcgg cgattactat gattttcacg tttccgaaga
     1381 cggcacttta acggtcgcgg tcggcgacgc caccggacac ggactcaaag ccggttcggt
     1441 cgtcaccgca acgaaaagcc ttttcaacgc cttcgcgggc gagtccgaca tcgcgcgcat
     1501 tttccaaaag acttccgccg cgctgaaaaa aatgaatctg cgcggtttgt ttatggcgat
     1561 ggcgatgct
//
LOCUS       DASWNE010000711         3016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_235234, whole genome shotgun sequence.
ACCESSION   DASWNE010000711 DASWNE010000000
VERSION     DASWNE010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235234"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="VGB68_13940"
     CDS             complement(<1..745)
                     /locus_tag="VGB68_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050390.1"
                     /translation="MQSSLLSRIFSISVILSLLVGLNVAVFAQSKPQKPDAPKGSQKK
                     NQRPVPKTEEELKKEEEERKRAEEEKNAVQDDEIVKIDTNVVNVDAVVYNKKTGQIIT
                     GLKKENFGVFENGVKQDITNFAMPEAPITVSLVVEYSKWTEVFGGAGTRGTFESGKYE
                     VIRPVAYFLSKFIKAPDDYASVIAFDMRPTTITDFTNDPNRLRATVDILLKNYPAFRE
                     NNLYDAVTFALVGGRGDSVVLENSKEETSE"
     gene            complement(923..1864)
                     /locus_tag="VGB68_13945"
     CDS             complement(923..1864)
                     /locus_tag="VGB68_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050391.1"
                     /translation="MKSKFFRVTLALSLLLCLCAMALADTIRLKDGSVIKGKIISFRD
                     GQFVVSIGDGARSRQMTFFADEVEAVEFDSLPLGATTTNPIKLTSPTTQNPRPQATPA
                     NNPTIPANTNTNNAGLNSNNNTNVIIVGKSNTNSQPTNSGGGANNDSNVNTAPSYTNS
                     TPTNPTPTGNVNANTIPVSTNTNPSTTRPKPIQLNVKVLADNTANGWTNSGFHVKKGQ
                     KFRVSGRGQISLGNGNFAKPEGIKSLADDKKLLPSEATGGLLAVIGDDNNDFIFIGAS
                     REFTATRDGMLFLGINENNLNDNSGSFEVTIEIEPIN"
     gene            complement(2273..>3016)
                     /locus_tag="VGB68_13950"
     CDS             complement(2273..>3016)
                     /locus_tag="VGB68_13950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0050392.1"
                     /translation="NCPQAIVVTDFDLHALWLCRNYERYFVALDETKFYLETLKFPAE
                     KISVTGIPIDPIFAETKDKRAMREKYNLDADKPTIILSAGGFGVGRIEILLKSLQTIR
                     QSVQILAMCGRNEELKNKLAQAADGNENCRVIPVGYTTEMDEYMSASDLVVGKPGGLT
                     TCEALAKGLIFVIVNPIPGQEERNSDHLLENGAGIRCNNLATLGYKVEQLLKDKERLN
                     FMKENALRFARPNAANDIAEILLKTETPA"
BASE COUNT          698 a          721 c          763 g          834 t
ORIGIN      
        1 attcggaggt ttcttccttg gaattttcca gcacgaccga atcgccgcgt ccgccgacga
       61 gggcaaatgt cacggcgtcg taaagattat tttcgcgaaa cgccggataa tttttcagca
      121 gaatatcgac cgtcgcgcgc aggcggttcg ggtcgttcgt aaaatcggta atcgtcgtcg
      181 gtcgcatatc aaaggcgatg accgaagcgt aatcgtccgg cgctttgata aatttcgaaa
      241 gaaaataagc gaccgggcga atgacttcgt attttcccga ttcgaatgtg ccgcgtgtgc
      301 cggcgccgcc gaaaacttcc gtccacttgc tgtattcgac gactaaagat acggttatcg
      361 gcgcttcggg catcgcaaaa ttggtgatgt cctgcttgac gccgttttcg aagacgccga
      421 aattttcttt ctttaaaccc gtgatgattt gtccggtttt cttgttgtaa accacggcgt
      481 cgacgttgac gacattcgta tcaatcttga caatctcgtc gtcctgaacg gcgttttttt
      541 cttcctcggc gcgtttacgt tcttcttctt cctttttcag ctcttcttcg gttttcggaa
      601 cgggacgctg gtttttcttt tggctgcctt tcggcgcgtc gggtttttgc ggtttcgatt
      661 gagcgaaaac cgcgacgttt aacccgacga gcagagataa gatgacactt atcgaaaaga
      721 tacgactaag gagacttgat tgcatattga tacctttcgg agaaaatcta ttgtcttaga
      781 ttccgatttt ccgcttttag ttggaagaag acaaataaga aattcaggat tcaggattca
      841 aaattcagga ttgtattttg aatccctaat cttaaatccc gaatcccgaa tcttaaatcc
      901 tgaatcttaa atcccgaatc ctttagttaa tcggttcaat ctcaatcgtc acttcgaacg
      961 agccggaatt gtcgtttaaa ttattttcgt taatgccgag aaacaacatt ccgtcgcgcg
     1021 ttgccgtaaa ttcgcgcgac gcgccgataa aaataaaatc gttgttgtcg tcgccgatga
     1081 cggcaagcaa tccgccggtc gcttccgagg ggagcagttt tttgtcgtca gccagggatt
     1141 tgatgccttc gggcttggcg aaattgccgt tgccgaggct gatctgaccg cgtcccgaaa
     1201 cgcggaattt ctgtcctttt ttcacgtgaa agcccgaatt cgtccagccg ttcgccgtgt
     1261 tgtcggcgag cactttaacg tttagctgaa tcggcttcgg tcgcgtcgtc gacgggttcg
     1321 tgttcgtgct gacgggaatc gtgttcgcgt tgacattgcc ggtcggcgtc gggttggtcg
     1381 gcgtcgagtt tgtataagaa ggcgctgtgt tgacgtttga atcgttgttt gcgccgccgc
     1441 cggaattggt cggttgcgaa ttggtgttgg atttgccgac gataatcaca tttgtattat
     1501 tgtttgaatt taagccggcg ttatttgtgt ttgtattggc tggaatcgtc gggttgttcg
     1561 ccggggtggc ttgcggtcgc ggattctgcg tcgtcgggct ggtgagctta atcgggttcg
     1621 tcgtagtagc gccgagcggc agcgaatcga actcgaccgc ttcgacctcg tcggcaaaaa
     1681 aagtcatctg ccgggagcgc gcgccgtcgc cgattgagac gacaaactgc ccgtcgcgaa
     1741 aactgatgat tttgcctttt ataacgcttc cgtctttcag ccgaatcgtg tctgccagcg
     1801 ccatcgcgca taaacacaaa agcaggctca atgccagggt cacgcgaaaa aatttggatt
     1861 tcaaaaacat attacctcca aaggatttca aattttcaga tttaaatggt gtcgaatttg
     1921 cgaccgtgca aagagatgtt ttaacgaaac cgttttcagg gaaagtggct tttaaaacta
     1981 attgccggaa caattttgag agttgaaaaa acagcgacgc acaaattcaa ggagattata
     2041 cagaaaattc tccgagttgt taaacaaaca ttgcggtttt atttgccgaa cgaccggaaa
     2101 gactgaaaat cagccgcaaa caaatacgaa agagcacgaa aaaactttgc cgccggcgat
     2161 acaaagaata agaaaaaaga aaattcagcc gcatataaac aaatggaaaa tttaaagagc
     2221 gtttttctta tcattttcca atatccgttt tccgttttcc ggacgcgctc gattacgcag
     2281 gcgtttccgt ctttaaaagt atttccgcga tgtcgttcgc cgcgttcggg cgggcaaagc
     2341 ggagagcgtt ttccttcata aaattcaatc tttccttatc tttcaaaagc tgttcgacct
     2401 tgtagccgag cgtcgccaga ttattgcagc ggatgcccgc gccgttttcc agcaggtggt
     2461 cggaattgcg ctcttcctga ccgggaatcg ggttgacgat gacgaaaatc aagcctttcg
     2521 ccagcgcttc gcacgtggtc aagccgccgg gcttgccgac gaccaaatcc gaagccgaca
     2581 tatattcgtc catctcggtc gtgtaaccga cgggaatgac gcggcagttt tcgtttccgt
     2641 ccgccgcctg cgcgagcttg tttttgagtt cttcgttgcg cccgcacata gcgagaatct
     2701 ggacgctttg ccggattgtt tgcagcgatt tcagcagaat ctcgatgcgc ccgacgccga
     2761 aaccgcccgc cgataaaata atcgtcggtt tatcggcgtc cagattgtat ttttcgcgca
     2821 tcgcccgttt gtctttcgtt tccgcaaaaa tcgggtcgat gggaatgccg gtcacggaaa
     2881 ttttttcggc ggggaatttg agcgtttcca gataaaattt cgtttcgtcc agcgcgacga
     2941 aataacgctc gtaattgcgg cacagccaga gcgcgtgcaa atcgaaatcc gtcacgacaa
     3001 tcgcctgcgg gcagtt
//
LOCUS       DASWNE010000712         1900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_200697, whole genome shotgun sequence.
ACCESSION   DASWNE010000712 DASWNE010000000
VERSION     DASWNE010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200697"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(386..1618)
                     /locus_tag="VGB68_13955"
     CDS             complement(386..1618)
                     /locus_tag="VGB68_13955"
                     /inference="COORDINATES: protein motif:HMM:NF018743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HEY0050393.1"
                     /translation="MKSFYLITALLVFCLSAYSFDESAAASNQARAKAKKIVTGAEQS
                     DEYLPYLKGKRVGILANQTTITTDGKHLVDFLLSKGINVVKVFGPEHGFRGNASAGVK
                     VADEKDPATGIKIISLYGAKRKPTREDMSDIDVMIYDVQDVGVRFYTNINVLRDVMES
                     VAEFKKELLVLDRPNPNGYLIDGPILDMSLKSGIGQFPVPIAHGLTIAEFAQMINGEG
                     WLAEKAVCPLKIVKVRNYSHEMEYVLPVKPSPNLNTQQSIMLYPSTCLFEGTILNHGR
                     GTYSPFAVLGSPLLKGKYDFSFTPVGIPGMSETPLHMNEVCYGLDLRNYDTKQLRKSK
                     KLNLKWMMELYKAYPEKEKFFDKSYSKQINDIDKLAGTREFKEQIKAGKTEQEIRQSW
                     EPGLSNYKKTRQKYLLYK"
     gene            complement(1695..>1900)
                     /locus_tag="VGB68_13960"
     CDS             complement(1695..>1900)
                     /locus_tag="VGB68_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050394.1"
                     /translation="LPVENSGRIMEKGMILQIDRFYTVAHSAFFVQFYLYSRRNTVMK
                     ISARNLLKGKIKSMEDSEKGYPA"
BASE COUNT          495 a          425 c          393 g          587 t
ORIGIN      
        1 gggctcgcgt tgtttgtatt acttttggga acaatttatc agctcgtagt tctaatgcaa
       61 atgttcgcga tactcgaccg agaacatttt tatttgactg agaaattagg tagaaaaaaa
      121 aatagcacga aaaaaaaaca attgtcaaca tatttttgag aaaaaaattc aagccgccaa
      181 aattgtcagt cggcgggcat ttatcggcga gataatcgac gcttggattt ctcagcttcg
      241 tcgattttac agccgaaact tcatcccttt tcaatctcgc tttaattcgg tttggataac
      301 cagactgtta ttttcaatag ctttgaataa ttttcggcac tgacaaaact aaatttacgc
      361 tcttgtagaa ttgcctgaac gcgcgttatt tatacagcag atatttttga cgcgtctttt
      421 tgtaattcga caagcccggc tcccagcttt gcctgatttc ctgttcggtt tttcccgctt
      481 tgatttgttc cttaaactct ctcgttccgg ctagcttgtc gatgtcgttg atttgtttgc
      541 tgtagctttt gtcaaagaat ttttcctttt ccggataagc tttgtaaagc tccatcatcc
      601 atttcagatt gagcttcttt gatttacgca attgtttggt gtcgtagttt ctcaaatcca
      661 agccgtaaca aacctcgttc atatgcagag gcgtctcgct cattccggga atgccgacgg
      721 gcgtaaacga gaaatcgtat ttacctttca acaaagggct gcccaaaacg gcgaacggcg
      781 aatatgtgcc tctgccgtga ttcagaatcg ttccctcaaa cagacaggtc gaaggataaa
      841 gcataatgct ttgctgcgtg ttcagattcg gcgaaggctt gaccggcaga acatattcca
      901 tctcgtgact gtaattgcga accttgacga ttttcaaagg gcaaaccgcc ttttcagcca
      961 gccagccttc gccatttatc atttgcgcga attcggcaat cgtcagcccg tgggcaatcg
     1021 gaacgggaaa ctgtccgatg ccggatttca agctcatatc caaaatgggt ccgtcgatta
     1081 aataaccgtt cgggttcgga cggtctaaaa cgagcagttc ctttttgaat tcggcgaccg
     1141 attccatcac gtctctgagg acatttatgt tcgtgtaaaa cctgactccg acgtcctgaa
     1201 cgtcgtaaat catcacgtca atatcggaca tatcttcacg agtcggtttt cttttcgcgc
     1261 cgtataacga aattattttt attccggtcg ccgggtcttt ttcgtcggcg actttaacgc
     1321 cggcgctggc gttgccgcga aagccgtgtt cgggtccgaa gactttgacg acgttgatgc
     1381 ctttcgacaa cagaaaatcc accagatgct tgccatcggt ggtaatggtc gtctgattag
     1441 ccagaattcc gactcgcttg cctttcaaat aaggcagata ttcgtcggat tgttccgcgc
     1501 ccgtcacgat ttttttagct ttagcgcgag cttgatttga agcggcggcg gattcgtcga
     1561 atgaataagc ggaaagacaa aaaacaagca atgccgtaat caaatagaat gatttcataa
     1621 attttaatta tcgaataatg gaattaaaaa gggatgaagc gaaaacttca tcctttaaaa
     1681 tttatctttg gattttacgc cggataacct ttctcgctgt cttccattga tttgattttt
     1741 ccttttaata aatttctcgc gctgattttc ataactgtat ttcgccgtga atataaataa
     1801 aattgcacga aaaatgccga atgcgcaact gtataaaatc ggtcgatttg caaaatcatt
     1861 cctttttcca ttatccgccc cgaattttca accggcaaat
//
LOCUS       DASWNE010000713         5382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_620795, whole genome shotgun sequence.
ACCESSION   DASWNE010000713 DASWNE010000000
VERSION     DASWNE010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5382
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620795"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..274)
                     /locus_tag="VGB68_13965"
     CDS             complement(14..274)
                     /locus_tag="VGB68_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050395.1"
                     /translation="MPKRSSSKRSRSKDENVLAKSVIDEIIELTESEAFQQAPEKNPA
                     AVMLGRRGGLKGGKARAEKLSAEERSEIARNAAIARWSKDEN"
     gene            complement(525..725)
                     /gene="thiS"
                     /locus_tag="VGB68_13970"
     CDS             complement(525..725)
                     /gene="thiS"
                     /locus_tag="VGB68_13970"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEY0050396.1"
                     /translation="MKVFINGETRDIESNLNLRELLRRFDLPSERIAVELNRRVVRKK
                     DWENIEIKDADKIEIVHFVGGG"
     gene            complement(754..1605)
                     /locus_tag="VGB68_13975"
     CDS             complement(754..1605)
                     /locus_tag="VGB68_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050397.1"
                     /translation="MTYHVQTEDKGVNTPLILSLVYDRGTILASKRSPYDDLLNGGLD
                     ERQLAERLQKQHHLICAAIKAGRIEDLRRMTMKESANKKKGLVAQKEIKVPAAEKPET
                     KREEIPMPELLTKNAAQPEQDFEPQLQSKPAAQTEEAIFEIPISILEEPIIEAVEIVE
                     EETILPVDAVEIINDFTKTETLVEEQLTIELLNSPTFKGGERKTLSILVHRGREESGT
                     GGAHVMVKVLGSSFRPLIFHAKTDSNGVAIVHLQLPQFKQGRAAILVKAMSGGEEVEL
                     RRVIQHN"
     gene            1966..2460
                     /gene="ndhC"
                     /locus_tag="VGB68_13980"
     CDS             1966..2460
                     /gene="ndhC"
                     /locus_tag="VGB68_13980"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEY0050398.1"
                     /translation="MSSVFDIGAYLPIVVMFVVAAGFAASQILVTQLIGPRKRTATKL
                     MPYECGKDPVGSAHDRFSVKFYMVGVAFLLFDIEVLFLIPFAVAFKSLMRDEISGAAF
                     GTIAFIEVMIFIATLIAGLVYVWKKGVFDWNLQARSEARAEARALTAATKRARTMQQE
                     KRAA"
     gene            2478..3110
                     /locus_tag="VGB68_13985"
     CDS             2478..3110
                     /locus_tag="VGB68_13985"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEY0050399.1"
                     /translation="MGLENTLFESLPDVITVKLDAVVNWARKSSLWPATFGLACCAIE
                     MMNTVSARHDLSRFGAETFRASPRQADVMIVSGRVSRKMAPVLRRIYDQMPEPKWVIS
                     MGACATTGGVFDNYAIVQGVDKIVPVDVYVPGCPPRPEMLVHAIMMLQDKIMKESVKD
                     RRDTFEEESRQSLVPGTLPVTMGSESITGSRRADTRPYTIPSKHERRKSS"
     gene            3178..3657
                     /locus_tag="VGB68_13990"
     CDS             3178..3657
                     /locus_tag="VGB68_13990"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEY0050400.1"
                     /translation="MSEQLLGYVEKLKAENAAWVADFKNTFGEVSVYVPRESIEDVCL
                     FLKTTHDFDLLSDICGADRGAEEDPRFEVNYHLFSTVHHNRLRLKVLLSEDAPNVPTV
                     TSVWRTANWHERETFDMFGVIFDGHPNLERILLPSDFDGHALRKDYPLRGYEPYSLT"
     gene            3735..4955
                     /gene="nuoD"
                     /locus_tag="VGB68_13995"
     CDS             3735..4955
                     /gene="nuoD"
                     /locus_tag="VGB68_13995"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEY0050401.1"
                     /translation="MSTATENLPGQFEVLDQPLESQMTISMGPQHPSTHGVLRLELVL
                     DGEMVVRAIPDIGYLHTGMEKLFEYKKYQQGIVITDRMDYLNPLGNNLAYVMAAEKLL
                     QIEIPERAQVIRVMMCELQRIASHMVWLGTHALDIGAMSVFFYCFRQREKILNLIEAA
                     SGGRMTPSYFRIGGLMMDLPAGFDRRCKQFLEDFPAALAQFETLVSGNTLWQSRTKDI
                     GYISREDAIDWGLTGPCLRGSGVELDLRRANPYSGYETYDFEIPVEPDCDVWARFRVR
                     MRELYESYKIVRQTLERLKPGPTKADAPKVVLPDRNDMRTHMDSLIHHFLIVAEGFNV
                     PVGEVYHAIEGSKGELGCYLRSNGGPKPDRVHFRGPSFVNLSALPAMSEGEMVADVVA
                     IIGSLDIVLGEIDR"
     gene            4983..>5382
                     /locus_tag="VGB68_14000"
     CDS             4983..>5382
                     /locus_tag="VGB68_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050402.1"
                     /translation="MLKNIFLKFALLILSFAAAGTANVFAQANSCAVKLDVSNFDRSG
                     AIKNAQATALNIENKQVYRSALKDGMPYFAQLTEGKYDLTVTKAGFKQTKESYTVWCA
                     QAEEGVLLALMLMEKGSSKETFTVPKFKFGD"
BASE COUNT         1262 a         1318 c         1381 g         1421 t
ORIGIN      
        1 ataacggttt agattaattc tcgtctttgc tccatctggc aatagccgca ttacgcgcaa
       61 tttcagagcg ttcttcggca gagagttttt cagctcgcgc cttgccaccc tttaagccgc
      121 cgcgacgacc tagcataaca gccgcaggat ttttctccgg cgcttgctga aaggcttcgc
      181 tttcggttag ctcaataatt tcatcaatta cacttttggc taatacgttt tcgtcttttg
      241 aacgacttcg tttactgctt gaccgctttg gcataataat tgattattgg cgcaatttat
      301 tgtagatgtc aagtttttta accctagatg ttgttgacta aaataattct acttgaaaag
      361 tttgtcaaga tattttttca aagacctaat ataattagat tataaattac gaattttttg
      421 cggaaaaagt tttattttgg ttagcaccac agatttaaga cactactaaa attagaaaat
      481 ttaatccgca attcgtaatt cgtaattttt aattcgtaat tgattcagcc gccgccgacg
      541 aaatgcacga tttcgatttt gtccgcgtcc ttgatttcga tattttccca atcttttttg
      601 cggacgaccc ggcgattcag ctcgacggcg atgcgctcgg aaggcaaatc aaaccggcgg
      661 agaagctccc gcaaattcaa attgctttcg atatctctgg tttcgccgtt gataaaaact
      721 ttcatcttcc aatcgcaaat ctaaaatcta aaatcaatta tgctgaatca ctcttcgcaa
      781 ttcaacttcc tcgccgccgc tcatcgcctt gaccagaatc gccgcgcgtc cctgtttgaa
      841 ctgcggcagt tgcaggtgga cgatggcgac gccgttgctg tcggtcttgg cgtgaaaaat
      901 caaagggcga aacgacgagc cgagaacttt gaccatcacg tgcgcgccgc ccgtgccgct
      961 ttcctcgcga cctcgatgaa ccagaatgct cagcgttttt cgctcgccgc ctttgaaggt
     1021 cggcgaattg agcagctcaa tcgtcagttg ttcttctacg agcgtttcgg tttttgtaaa
     1081 atcgttgatg atttcgaccg cgtcaaccgg cagaatcgtt tcttcttcga cgatttcgac
     1141 cgcttcgata atcggttctt cgagaatgct gatgggaatt tcaaaaatcg cttcttcggt
     1201 ctgagccgcc ggtttagact gaagctgcgg ctcgaagtcc tgctccggct gagcggcatt
     1261 tttggtcagc agctcgggca tcgggatttc ttcccttttc gtttccggtt tttccgccgc
     1321 cggaactttt atttcctttt gcgcgaccag accttttttc ttgttggcgg attccttcat
     1381 cgtcatccgc cgcaaatctt ctatgcgacc ggcttttatc gccgcgcaaa tgaggtgatg
     1441 ctgcttttgc aggcgctcgg cgagctgtcg ttcgtcgagc ccgccgttga gcaaatcgtc
     1501 gtatggcgag cgtttactag ccagaatagt gccgcggtca tagaccagcg acaaaatcag
     1561 cggcgtattc acgcccttgt cctcggtctg gacgtgataa gtcactccgt tatattcaat
     1621 gtctgtatta aaacccgtaa tcacggcaat ttggcgagaa aggcggcgaa tttcagacgc
     1681 attttgcgtc ctttatgaaa gtaacataag cgtttacgga cggtcaacga ataaatgact
     1741 ttgaagactc tgaaaagcgg agagttatgc gccggattga tttttttttt ataaaagtta
     1801 tttcttttga ttctcctaac tctccggact ctctatactc ttgactctcc gtctggcttc
     1861 acatacgatt aacttgtgcc ataattctca agctgttttt gacgagaatt tatcgtctat
     1921 cgagcgcatt tttgaagcaa caagagagga ctcaggagaa tctaaatgtc atcagtattt
     1981 gatattggag cgtatcttcc gattgtcgta atgtttgtcg ttgcggcagg ttttgcggcg
     2041 agccagattc tggtcacgca attaatcgga ccgcgaaaaa gaacggcgac gaaattgatg
     2101 ccgtacgagt gcggtaaaga cccggtcggg tcggcgcacg accgattttc ggtcaagttt
     2161 tatatggtgg gcgtggcgtt tctgctgttc gacatcgaag ttttgttttt gattcccttt
     2221 gcggtggctt tcaagagctt gatgagagat gaaatcagcg gcgcggcttt cggcacgatt
     2281 gcttttatcg aagttatgat ttttatcgcc accttaattg ccggtcttgt ttatgtttgg
     2341 aaaaaaggcg tgttcgactg gaatttgcag gcgcgttccg aagctcgcgc cgaagccaga
     2401 gcattgaccg ccgctacaaa aagagcgcgc acgatgcaac aggaaaaacg cgccgcttga
     2461 gaatttggag ttgaataatg ggtttagaga atacactttt tgaatcgtta cctgatgtta
     2521 ttaccgtaaa gctcgacgcg gtcgtcaact gggcgagaaa atcctcgctc tggcccgcta
     2581 cattcggtct ggcctgctgc gccatcgaga tgatgaacac ggtctccgcc cgtcacgacc
     2641 tttcgcgttt cggcgcggaa acatttcgcg ccagcccgcg gcaggcggat gtgatgattg
     2701 tttcgggacg cgtctcgcgt aagatggctc cggttttaag acgcatctat gaccagatgc
     2761 ctgagccgaa atgggttatc tcgatgggcg cctgcgcgac gacgggcggc gttttcgaca
     2821 attacgcgat tgtgcagggc gtcgataaaa tcgttccggt cgacgtgtat gttccgggct
     2881 gcccgccgcg ccccgaaatg cttgttcacg ccattatgat gctgcaggac aagattatga
     2941 aggaatcggt caaagaccgt cgcgatactt ttgaggaaga atcccggcag tcgctcgtgc
     3001 ccggcacttt gcccgttacg atggggagcg aatcgataac cggctcgcgc cgcgccgaca
     3061 cgcgtcccta cacgattccg tcgaaacacg aacgaagaaa gtcttcgtag taaaatgttt
     3121 taatgagcgg tgaaagattt acggaaaact tcaccgcctc tttttgtata agaaaatatg
     3181 tccgaacaat tgcttggata cgtcgaaaaa ttgaaagccg aaaacgcggc gtgggtcgcc
     3241 gattttaaaa acactttcgg cgaagtcagc gtctatgtgc cgcgcgagtc aatcgaagac
     3301 gtctgtcttt ttttaaaaac gacgcacgat ttcgaccttt tatcagacat ttgcggagcc
     3361 gaccgcggcg cggaagaaga cccgcgcttc gaggtcaatt atcatttgtt ttcgaccgtt
     3421 catcataatc gcctgcgcct gaaagttttg ctttccgaag acgcgccgaa cgttccgacc
     3481 gtgacaagcg tctggcgcac ggcaaactgg cacgagcgcg aaacgttcga tatgttcggc
     3541 gtaattttcg acggtcatcc gaatctggaa agaattttat tgccgtccga ttttgacggg
     3601 cacgcgctgc gaaaagatta tccgctgcgc ggatatgagc cgtacagctt gacgtaaagt
     3661 aaatcgcaca aatcgtgaat cgtaagtaga aacgagaagc gatttacgat ttacgattca
     3721 cgatttaaga aattatgagc actgcaaccg aaaatttacc gggtcaattc gaagttttag
     3781 accagccgct ggaatcgcag atgacgattt cgatgggacc gcagcatcct tcgacgcacg
     3841 gcgttctgcg tctggagctc gtgctggacg gcgagatggt cgtccgcgcc attcccgaca
     3901 tcggctatct gcacacgggg atggaaaagc ttttcgagta taaaaaatac cagcagggca
     3961 tcgtcatcac cgaccggatg gattatttaa atccgctcgg caacaatctc gcctacgtga
     4021 tggcggcgga aaagcttctg caaatcgaga ttcccgaacg cgcgcaggtt atccgcgtga
     4081 tgatgtgcga gctccagcgc atcgcgtcgc atatggtctg gctcggcacg cacgcgctcg
     4141 acatcggcgc gatgagcgtc ttcttttact gttttcgaca gcgcgaaaag attctgaatt
     4201 tgattgaagc cgccagcgga ggcagaatga cgccgagcta ttttcgcatc ggcggcttga
     4261 tgatggattt gcccgccgga tttgaccgtc gctgtaaaca gtttctggaa gatttccctg
     4321 ccgcgctcgc gcaatttgaa acgctcgtct ccggcaacac gctctggcag agccgcacca
     4381 aagacatcgg ctacatcagc cgcgaagacg cgattgactg gggcttgacc ggaccttgcc
     4441 tgcgcggcag cggcgtcgag ctcgatttgc gccgcgccaa cccgtattcg ggttacgaaa
     4501 cctatgattt tgaaatcccg gtcgagccgg actgcgacgt ttgggcgcgt ttccgcgttc
     4561 gtatgcgcga gctttacgaa tcatataaaa tcgtccggca gacgctggag agattaaagc
     4621 cgggaccgac gaaagccgac gcgccgaaag tcgtgctgcc cgaccgcaac gatatgcgga
     4681 cgcatatgga ttcgctgatt caccactttt tgattgtcgc cgaaggcttt aacgttccgg
     4741 tcggcgaagt ttatcacgcc atcgaaggct cgaaaggcga gctcggctgt tacctgcgct
     4801 caaacggcgg accgaaaccg gacagagttc attttcgcgg tccgagcttt gtcaatcttt
     4861 cggcgctgcc cgcgatgagc gaaggcgaaa tggtcgccga cgtggtggcg ataatcggct
     4921 cgctcgatat tgttctgggc gagatcgacc gttgattttc cgaaaggcgg aagaagataa
     4981 aaatgctgaa aaatattttt ttaaaattcg ccttattaat tctttcgttt gccgctgcgg
     5041 gcacggcgaa tgttttcgcg caggcgaatt cgtgcgcggt gaagctggat gtttcaaatt
     5101 tcgaccgcag cggagcgatt aaaaacgcgc aggcgacggc gttgaatatt gaaaacaagc
     5161 aggtttatcg ttccgcgctg aaagacggga tgccgtattt cgcccaactg acggagggca
     5221 aatacgacct taccgtcacc aaagcgggtt tcaaacagac gaaagagagc tacacggttt
     5281 ggtgcgcgca ggctgaagaa ggcgttttgc tggcacttat gttgatggaa aagggcagct
     5341 caaaggaaac tttcacggtt ccgaaattta aattcggaga ta
//
LOCUS       DASWNE010000714         2610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_483109, whole genome shotgun sequence.
ACCESSION   DASWNE010000714 DASWNE010000000
VERSION     DASWNE010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483109"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(321..2237)
                     /locus_tag="VGB68_14005"
     CDS             complement(321..2237)
                     /locus_tag="VGB68_14005"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY0050403.1"
                     /translation="MNGLNTASLINLLGFTVGIALYGLLLVMVMRHRKTQKFSSLDFL
                     LLATSLLGVLWNLGELSVFVWRDFGQSEVSPVLTAISYSALGFLPSVVAHSAWKNAEK
                     ETRNVRWLAIPAYGLSFVAAVLHFQSALFAGRAPSNSALQILTFGSLALLVGLLIFNF
                     KQRLEKKLVWASALLVFAVSALHLSGDAEGNSWFIELVAHQSSLPLALVILYQDFRFA
                     FADLFLKRALSLILLTLTAFGLYSFIAAPLLRYHETHDRNDVHAVSLIITLWVVTALV
                     YPSLHKFAVWLVDKIILRRTNYETLRAEIAREIEKRESGEEILREVCRRLAPALTAKS
                     ANWSESLERKINMPFVNFTPHDAEIFVPVAEAPFYKIGLKDFAGGRHLLSDEIKTLEA
                     VGLLTARRIDAVRVTHERYTREIREREFSRLATEAQLSALRAQINPHFLFNALTTIGY
                     LIQTAPDKAFSTLMRLTQLLRGVLRSTGEFSTLEAELKLIEAYLEIEKTRFEERLRVR
                     IEVPPDLLKARVPSLILQPLVENAIKHGISESKSGGEIGIYAELENESNGAFLKLTVS
                     NTGKGLDRAKFAERQILGVGLNNIKQRLQNYYGNAASFNIESKNGTQAKISLPVAATE
                     NLTQRREEARAQRK"
BASE COUNT          653 a          662 c          653 g          642 t
ORIGIN      
        1 gacgatttcc acgtcttcaa attcctgcaa aatatttttc aggaacatcc gcgccggacg
       61 ctcgtcgtcg gcgatgacga ctcgcagttt attcataatt ttttagccac agagggcgca
      121 gagaacacag agggaaatta accacagatt tttcacagat aaacgcggat tcaaacagga
      181 tggacaggat ttaaaggata aattgttttt caaatcaaga ttcgagtttt attcagaact
      241 tttgaataaa aaagtttcca tcctgcctat cctgttcatc ctgttaaatt attcgcgtta
      301 atccgtgtga atctacggtt tcatttcctt tgcgcccttg cctcctcgcg cctttgcgtt
      361 aaattttcgg tcgccgcaac cggaagcgat attttcgcct gcgtgccgtt tttgctttcg
      421 atattaaagc ttgccgcgtt tccgtaataa ttctgcaggc gctgtttgat gttgttcagc
      481 ccgacgccta gtatttgccg ctcggcaaat ttcgcccggt caagaccttt gcccgtgttg
      541 gaaacggtta atttcagaaa agcgccgttg ctttcgttct caagctcggc gtaaatcccg
      601 atttcgccgc cgcttttcga ttccgaaatg ccgtgtttaa tggcgttttc gacgagcggc
      661 tggagaatca gcgacggaac gcgcgctttt aataaatccg gcggcacttc gatgcgaacg
      721 cgcaggcgtt cttcaaatct ggttttttcg atttccagat acgcttcaat cagttttaat
      781 tcggcttcga gcgtcgagaa ttcgccggtc gagcgcagaa cgccgcgtaa aagctgcgtc
      841 aggcgcatca atgtcgaaaa cgctttgtcg ggcgcggttt gaatcagata accgatggtc
      901 gtcagcgcat tgaatagaaa atgcggattg atttgggcgc gcagcgcgga aagctgcgct
      961 tcggtcgcca gcctggaaaa ttcccgctcg cggatttcgc gcgtgtagcg ttcgtgtgtg
     1021 acgcgcacgg cgtcgatgcg ccgcgccgtt aaaagtccga ctgcttcgag cgttttaatt
     1081 tcgtcggaga gcagatgccg cccgcccgca aaatctttca aaccgatttt ataaaacggc
     1141 gcttcggcga cggggacgaa aatttccgca tcgtgcggcg tgaaattgac gaaaggcata
     1201 tttattttcc tttcgaggct ttccgaccag tttgccgatt tggcggtcag cgccggcgcg
     1261 agacggcggc aaacttcgcg caaaatttct tcgccggatt cgcgcttttc gatttcccgg
     1321 gcgatttcgg cgcgcagcgt ttcgtaattc gtccggcgca gaattatttt atcgaccagc
     1381 cacacggcga atttgtgcag cgaagggtag acgagcgccg tcacgaccca gagcgtgatg
     1441 attaagctga ccgcgtgcac gtcgttgcgg tcgtgcgttt cgtgataacg cagaagcggc
     1501 gcggcgatga agctgtataa gccgaaagcc gtcaaggtca gcaaaatcag ggacaaagcg
     1561 cgttttaaaa ataaatcggc gaaggcgaaa cgaaaatcct ggtacaaaat gacgagcgcc
     1621 agcggcagac tcgattggtg cgcgaccagc tcgataaacc acgaatttcc ttccgcgtcg
     1681 ccgctcaggt gcagagccga aaccgcgaaa accagcaaag cgctcgccca gaccagtttt
     1741 ttttcgagcc tctgcttgaa gttaaaaatc aataatccga ccagcagggc gagcgagccg
     1801 aaagttaaga tttgcagcgc cgaattcgac ggcgcgcgcc ccgcaaacaa cgccgattga
     1861 aaatgcagaa cggcggcgac aaaactcaga ccgtaagcgg gaatcgccag ccagcgcaca
     1921 tttctggttt ccttctccgc gtttttccag gcggaatgcg cgacgaccga cggcagaaaa
     1981 ccgagcgcgg aataagaaat cgccgttaaa accggcgaca cttcgctctg cccgaaatcg
     2041 cgccagacga aaaccgaaag ctcgccgaga ttccacaaaa cgccgagcaa cgaagtcgcc
     2101 agaagcagaa aatccagcga agaaaacttt tgcgttttcc ggtgacgcat caccatcacg
     2161 agcagcagcc cgtaaagcgc gatgccgacc gtaaagccga gcagattgat gagcgaggcg
     2221 gtatttaatc cgttcattgg taaaacttca aattaaattt gaaattagag tttagcaaga
     2281 tttttaaatt tcggttaata aaatagccac gaaatttcac gaaaaaacac gaaaattatt
     2341 tattcctttt tcgtgcctgt tcgtgttatt tcgcggctat ttcttttagc ggcgattttt
     2401 cgatgattaa tatttcatca attccgcgac ggctttctca acgtcttcaa tctgcaacgg
     2461 ctgagatgac gtggggcgaa acaccgcgca acttggtaaa tgaaacaacg tccgtgtttc
     2521 gccctcacgt ccattgaatt gttcggtgta tggttaatcg aagaaaggcg ttgtccaaac
     2581 acgctctttc caatttaacc aagcacgttg
//
LOCUS       DASWNE010000715         1609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_555184, whole genome shotgun sequence.
ACCESSION   DASWNE010000715 DASWNE010000000
VERSION     DASWNE010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555184"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="VGB68_14010"
     CDS             complement(<1..928)
                     /locus_tag="VGB68_14010"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEY0050404.1"
                     /translation="MNKIALAIHGGAGTILRSEMTAELEKDYRGGLTGALQTGWKILQ
                     KGGNALDVVEAAVVELENFPLFNAGKGAVFTHEGKNEMDACLMNGASLRAGAVAFVKN
                     VKNPIKLARLVMERTPHVLLAGRGADEFAREMQIELEDDKYFYTEFRYRQLLKAREAG
                     RIQLDHTASEPALIEKEIQADENLKAEIENAKSLGTVGAVACDLRGNVAAATSTGGMT
                     NKKFGRVGDTPLVGAGTYADNRTCAVSCTGHGEFFMLGVTAYDVAARMKYKNLNLADA
                     ARETVEHLTEIGGEGGLIAVDSQGNVALPFNSD"
     gene            complement(1079..>1609)
                     /locus_tag="VGB68_14015"
     CDS             complement(1079..>1609)
                     /locus_tag="VGB68_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEY0050405.1"
                     /translation="KEAIDELHRQKIEVVMMTGDNAKTAEAVARRLGIDKVFADVLPE
                     QKAEKVKELQALGKIVAMAGDGVNDAPALAQAEVGIAMGTGTDVAMESADITLLKGDL
                     NGILRARRLSQATMKNIRQNLFFAFIYNLVGVPVAAGVLYPLGILLSPMLASAAMTFS
                     SVSVILNALRLRSLKL"
BASE COUNT          333 a          477 c          401 g          398 t
ORIGIN      
        1 catcggaatt aaacggcaga gcgacgtttc cctgcgaatc gaccgcaatc aaaccgcctt
       61 cgccgccgat ttccgtcagg tgctcgacgg tttcgcgcgc ggcgtcagcc agattcagat
      121 ttttatattt catccgcgcc gcgacgtcgt aagccgtcac gccgagcata aaaaattcgc
      181 cgtgtccggt gcaggaaacc gcgcacgtac ggttgtcggc gtaggttccc gcgccgacga
      241 gcggagtatc gccgacgcga ccgaactttt tattggtcat tccgccggtc gaggtcgccg
      301 ccgcgacatt gcctctcaaa tcgcaggcga ccgcgccgac cgtgccgagc gattttgcat
      361 tttcgatttc ggctttcaaa ttctcgtccg cctgtatctc cttttcaatc aaagccggtt
      421 cgctcgccgt gtggtcgagc tggatgcgcc cggcttcgcg cgcttttaaa agctgccggt
      481 agcgaaactc ggtgtagaaa tatttatcgt cttcaagctc gatttgcatt tcgcgcgcaa
      541 attcgtcggc gccgcgcccg gcgagcagaa cgtgcggcgt ccgctccatc acgagccgcg
      601 ccagctttat cggattttta acgtttttga caaaagcgac cgcgccggct cttaagctcg
      661 cgccgttcat caggcaggcg tccatctcgt ttttgccttc gtgcgtgaaa accgcgcctt
      721 ttccggcatt gaaaagcggg aaattttcaa gctcgacgac cgccgcttcg acgacgtcaa
      781 gagcattgcc gcccttttgc agaattttcc agcccgtttg cagagcgccc gtcaagcctc
      841 cgcgataatc cttttccagc tcggcggtca tctcgctccg cagaatcgtg cccgcgccgc
      901 cgtgaattgc cagtgcaatt ttattcattt aattattttg ccacagagga cagcgggagc
      961 atagagaaat taacctggat aaagaagata aaccgggatt taaagaaaga agaaaaaatt
     1021 ttataaatct ccttcatttt ctctctcccg gttaattttc ctttattttc tttccgcatt
     1081 acagctttaa gctcctgagc cgcaaggcat tcagaatgac cgaaaccgag ctgaaagtca
     1141 tcgccgcgct tgccagcatc gggctgagca aaatgccgag cgggtataaa acgcccgccg
     1201 cgacgggcac gccgaccaga ttgtaaataa aagcgaaaaa gaggttttga cgaatattct
     1261 tcatcgtcgc ctgcgataat cgacgcgccc gaaggatgcc gttcaaatcg ccttttaaaa
     1321 gcgtgatgtc cgccgattcc atcgccacgt ccgtgccggt tcccatcgcg atgccgacct
     1381 cggcttgcgc cagagcgggc gcgtcgttga cgccgtcgcc agccatcgcc acgattttcc
     1441 ctagtgcctg cagctccttc actttttcag ccttttgttc gggcaaaacg tcggcaaaaa
     1501 ctttgtcgat gccgaggcgt cgagcgacgg cttcagcggt tttcgcgtta tccccggtca
     1561 tcattacgac ttcgattttc tgcctgtgca gttcgtcaat tgcttcttt
//
LOCUS       DASWNE010000716         3502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_335908, whole genome shotgun sequence.
ACCESSION   DASWNE010000716 DASWNE010000000
VERSION     DASWNE010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335908"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..1064
                     /locus_tag="VGB68_14020"
     CDS             81..1064
                     /locus_tag="VGB68_14020"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF008854.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HEY0050406.1"
                     /translation="MALLTIRDALNQALREEILRDENVFIMGEEVAEYDGAYKVTRGL
                     WKEFGDKRVIDTPITELGFAAVGVGAAMAGLRPVIEFMTFNFSILASDQIINHAAKML
                     YMSGGQFKVPIVFRGPNGSAYQVSSQHSQALEAFYANFPGLKVVMPSTAADAKGLLKS
                     AIRDDNPVVFLEQERMYGLKGEVPEDEDFTIPLGVADVKREGTDCTIVARSMTVPLAL
                     QAAEQAQKEFGVSVEVVDPRTIKPLDIETIVGSVKKTNRLIVVEESHAFASVGAEISH
                     QVMENAFDYLDAPVKRISCAEAPMPYAKNLEELALPDVPKIVAAIKEVCYL"
     gene            1153..1539
                     /locus_tag="VGB68_14025"
     CDS             1153..1539
                     /locus_tag="VGB68_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050407.1"
                     /translation="MKQTKAVLFDSFTVFDAELNSSRIDKLLNEAGKNSKNKAVIFVY
                     CGRACKYGEVEAHFRGIKIKMKLRGVPADRYLILAGGYREQTETELWFVPENACLPIP
                     NSTVDIQDVRFKDKFKGTFVPYECCP"
     gene            1572..3029
                     /locus_tag="VGB68_14030"
     CDS             1572..3029
                     /locus_tag="VGB68_14030"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HEY0050408.1"
                     /translation="MPKLSPTMEEGQISRWLKAEGDDIEANETIAEVDTDKATMEMTS
                     LEPGTLLKIIVPAGDNARLGQTIGIIGAKGEDFSALLDDASSSNAGSNGGGDGANVTS
                     EDKARRESEVEDIPKESKPPEAAGAESKSAAAVAAPAQEGSVSETAGRSYDYQRPQAV
                     EQAQSPQQPPTATQPQSESDGGGGSGGRMIVSPIAARMAAENGVDLKSVKGSGPNGRI
                     IKRDIEAAMESVQPSAAGAQPEQTQTAPSFAQASEIQNASRFREENTSRMRQVIAQRL
                     AQSIGPIPTFYLTVEIEMDKALEFRKQINASVKEDEKVSVNDIIVKVAAQALTKHPFV
                     NSSYQDKTIRFYEDADIGVAVAIDEGLITPVVRGANRKGITEISREIKEMAARAREKR
                     LQPEEYTGATFSISNLGMFGIREFTAIINPPEAAIIAVGAATPSAVVRDGEITTRNVM
                     NVTMSCDHRVIDGATGAKFLQTFKQMLENPILMLM"
BASE COUNT          933 a          890 c          933 g          746 t
ORIGIN      
        1 agcagtaact tacggtttaa aaagaagtcg tgagcgataa aaaccgctca cggctcaccg
       61 cttactgctc actaaaaaat atggcattac ttacaatccg cgacgctctg aatcaggcgc
      121 tgcgcgaaga aattttaaga gacgaaaacg ttttcataat gggcgaggaa gtcgccgaat
      181 acgacggcgc ttataaagtg acgcgcgggc tttggaagga attcggagac aagcgcgtga
      241 ttgacacgcc gattaccgaa ctcggttttg ccgccgtcgg cgtcggcgcg gcgatggctg
      301 gactgcgccc ggtgatcgag tttatgacgt ttaatttttc gattttggcg tcagaccaga
      361 ttatcaacca cgcggcgaaa atgctttata tgtcgggcgg gcagtttaaa gttccgatcg
      421 tctttcgcgg accgaacggc tcggcttacc aggtttccag ccagcactcg caggcgctcg
      481 aagcgtttta cgccaatttt ccggggctga aagtcgtgat gccttcgacc gccgccgacg
      541 cgaaaggtct tttaaaatcg gcaatccgcg acgataatcc ggtcgttttt ctcgagcagg
      601 aaagaatgta cggcttgaag ggcgaagttc ccgaagacga agatttcacg attcctctgg
      661 gcgtcgccga cgtcaagcgc gaaggaaccg actgcacgat tgtcgcgcgc tcgatgaccg
      721 ttccgctcgc tttgcaagcc gccgagcagg cgcagaaaga attcggcgtt tcggtcgagg
      781 tcgtcgaccc gcgcacgatt aagccgctcg acatagagac gattgtaggc tcggtgaaaa
      841 agacgaatcg gctgattgtc gtcgaagaat cgcacgcttt cgcctcggtc ggcgcggaaa
      901 tttcgcatca ggtgatggaa aacgccttcg attatctgga cgcgccggtg aaaagaatct
      961 cgtgcgccga agcgccgatg ccgtatgcga aaaatctgga agaactggcg ctgccggacg
     1021 ttccgaaaat cgtcgcggca atcaaagaag tctgttattt gtagttttat gcgaggttta
     1081 atttttgttt cgtattgcct ggtattttct gtctggacaa tcttcggaca gcaaaatttc
     1141 ctcgcactgg atatgaaaca gacaaaagcc gtactgtttg attcatttac agtttttgac
     1201 gctgaactta attcgtcacg gattgataag ttgctaaatg aagcaggtaa aaattcaaaa
     1261 aataaagctg ttatttttgt ttactgcggg cgggcttgca aatatggtga agtcgaggca
     1321 cattttcgcg gtatcaaaat aaagatgaag ctgagaggcg tgcccgccga ccgttatctg
     1381 atattggctg gcggttatcg tgaacaaaca gaaacggaat tatggtttgt tccggaaaat
     1441 gcctgtctgc cgattccgaa ttcgacagta gatattcaag atgtaagatt caaggataaa
     1501 ttcaaaggca cttttgttcc ttacgaatgt tgtccttaat taatagaaaa aaaatggcga
     1561 acaaaatttt aatgcccaag ctttctccga cgatggagga aggacagatt tcacgctggc
     1621 tcaaagccga aggcgacgat atcgaagcca acgaaacgat tgccgaagtc gataccgaca
     1681 aggcgacgat ggaaatgacc tcgctcgaac cgggaacgct tttgaaaatc atcgtcccgg
     1741 cgggcgacaa cgcccggctc ggtcagacca tcggcatcat cggcgcgaaa ggcgaagatt
     1801 tttccgcgct gctcgatgat gcttcttcgt caaatgcggg aagcaacggc ggcggagacg
     1861 gcgcaaacgt cacttccgaa gataaagcca gacgggaatc cgaagttgag gacattccga
     1921 aagaatccaa gccgcccgaa gctgccggcg ccgaatccaa atctgctgct gctgttgccg
     1981 cgcccgcgca ggaaggcagc gtttccgaaa ccgccggacg cagctacgat tatcagcgcc
     2041 cgcaggctgt cgaacaggct caaagcccgc aacagccgcc gaccgcaact cagccgcaaa
     2101 gcgaaagcga cggcggcgga ggttcaggcg gtagaatgat tgtttcgccg atcgcggcgc
     2161 gtatggcggc tgaaaacgga gtcgatttga aatccgtcaa aggctccggt ccgaacggtc
     2221 gcatcatcaa acgcgacatc gaagcggcga tggaaagcgt tcagccgtca gccgccggcg
     2281 ctcagccgga gcagacgcag accgcgccga gctttgcgca agcttccgaa atccaaaacg
     2341 cttcgcgctt ccgcgaagaa aacacttccc ggatgcgaca ggtcatcgcg cagcgtctcg
     2401 cccaatccat cggaccgatt ccgacgtttt atctgacggt cgaaatcgag atggacaagg
     2461 ctctggaatt tcgcaagcaa atcaacgcca gcgtgaagga ggacgaaaaa gtttcggtca
     2521 acgacattat cgtcaaagtc gccgcgcagg ctttaaccaa acatcctttc gtcaattctt
     2581 cctatcagga caaaaccata cgcttctacg aagacgccga catcggcgtc gccgtcgcca
     2641 tcgacgaggg gctaataacg ccggtcgtgc gcggcgcgaa ccgcaaaggc atcaccgaaa
     2701 tttcccgcga gataaaggaa atggcagcgc gcgcccgcga gaaacggctt cagcccgaag
     2761 aatacaccgg cgcgacgttt tcgatttcca atctcggaat gttcggcatc agggaattca
     2821 cggcaatcat caacccgccc gaagccgcca tcatcgccgt cggcgcggcg accccgagcg
     2881 cggtcgttcg cgacggcgaa atcacgacca gaaacgtaat gaacgtcacg atgagctgcg
     2941 accaccgcgt catcgacggc gcgacgggag cgaagttttt gcagaccttc aagcaaatgc
     3001 tcgaaaatcc gattctgatg ctgatgtaaa tttaattaag aattaaaaat tacgaattac
     3061 gaatcgtaaa agaaaggcga aagatgacgt ttaacagccg tcactttcgc cttttttaac
     3121 aataagccat tgtgcggagc aataaatcag ccgtgtgtaa aagtaagcgg ctcctgcgga
     3181 aaagttacgc gcgtgtagag aagcaacccg ccaatgtatg aaagttactc gctcgtgcaa
     3241 aaaagtaacc tgctcgtgta taaaagtaag ctgcccgtgc aggattgtaa cccggtcgcg
     3301 cggaaaagtt actgagccgt gtataaaagc cgctcggtcg tgcaagactt ttacctgctc
     3361 gcgcaaaact cgatttcgct cttgtcggaa agtcagccgt atcgaaataa tgcagcgggc
     3421 aatgataaag ggtaaataat aaatgttttc attcgtctcc gccgacgatt tgcttaaaca
     3481 cttcatcgtc gcgtatcgaa tc
//
LOCUS       DASWNE010000717         3974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_163656, whole genome shotgun sequence.
ACCESSION   DASWNE010000717 DASWNE010000000
VERSION     DASWNE010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163656"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VGB68_14035"
     CDS             <1..436
                     /locus_tag="VGB68_14035"
                     /inference="COORDINATES: protein motif:HMM:NF021080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="double-CXXCG motif protein"
                     /protein_id="HEY0050409.1"
                     /translation="FGHSVGRAAGQLSGFVWRAWWTISLESNALQKLVNAPLNLPVAV
                     KAELKFKKDVQEVFEFDLPLKGELINGVYDGIQIKYCYACGRDSASKPEEILINRLSI
                     PDDTDIFRVANFTTIILATERFVETVTNLNIKGAVFEEVKIV"
     gene            complement(468..>3974)
                     /locus_tag="VGB68_14040"
     CDS             complement(468..>3974)
                     /locus_tag="VGB68_14040"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY0050410.1"
                     /translation="RVAVNQTHVAFTYAGKIWLVERSGGAARRLTNTPNEETNPIFSP
                     DGKQIAFSRLNGSDWDVFVISADGRGEARRVTIMPEDDLVTAWTPDGREVIFETTRDQ
                     EGVTRLYKMAIENGTLATPLPMQQAYQGVFSPDGNFIAFNPRLGFGEWRYYRGGAIAP
                     IWITDLKTGALEKLPNPNYNDRSPQWVGDKIYFTSDRTGIFNLFAHDRKTRQVRQLTN
                     FKDFGVRAAAATTDAICFVHGGRLHLLDLNTNQTRAIDVSVAPDTAELRPRNVQAIRF
                     LEQILPSDSGDRIVFGARGEVLLFDTANGNSKNLTNSSGVAERYPVISPDGKSVAYFS
                     DESGEYALHVRSLENDSVKKIAVEPKPSFYWETIWSPDSKKIAFSDRQLNLWTADAEK
                     GTVEKIDTSVYSAQEGWTPNFSPDSRYLAYAKRLRNRAGTVYIRDLAQKKNVQITDGV
                     THTQLPVFDPNGKYLYFLSSANAGTSEFNWGVLNGVLANPLIVRRVHAFILSKDAPSP
                     LLPNGQPNPDAKINEAVSQVRIDFDNASARFVNLPLPQRDYSQLAAGKAGKLHLVVSE
                     WSKAPGDFTEPSQSRAVYLFDLAKGGRMEKIVDEINAVDITRDGSRILYRKGRDYFLT
                     GAETPAKPEDGRQDFNKMEVRVNPSEEWRQMFHESMRIMRDWFYDPNYHGQNLALLER
                     EYSAYLPTVTRRGDLNGLMRQMLGHVSVSHLGVGGGDSLPPQGGGNRIGLLGADYEIA
                     DGHYRFKKIYRSTPFTVANGSFSAPLDQPGVDVREGDFLLEVNGQKIEASKNILSYFE
                     NAAGRPTRITVSNNANGSNARAFTVYPASGENRLRRANWAEDNRKTVERLSGGKLGYI
                     FIEGYNPDGISNAIRGLSGYADKAGVIIDQRYNGGGITPDYLIEWMLRKPLYYYMFRG
                     GDDIPTPVNPAPPVKVLITNEFNGSAAETGAFMFKLGKVGQIVGKRTYGAGIGPYFFT
                     PRLIDNGGVQLPNRAAYNPNGTWEVENYGVVPDFDVEITPQDSMAGKDSQLEKAVEVA
                     LAQIQKNPVVQPKRPAFPIHPFDTLQTNSTGSLVLPKPGSAFPAPVEIKPTVGGKFAD
                     FAGKFDTPMGAVTFQQEGEKLIGNAGGERLELSPDASTKDKFVAQSASVTVTFERDAA
                     GKIAGVTIVIPSGREIKGKKID"
BASE COUNT          840 a         1088 c         1091 g          955 t
ORIGIN      
        1 atttggtcat tccgtcggtc gggcagccgg gcagcttagc ggttttgttt ggcgagcttg
       61 gtggacaatc tcgcttgagt cgaatgccct gcaaaaactc gtaaacgctc ctcttaatct
      121 gcctgtcgcc gtaaaagcgg aattaaaatt taagaaagac gttcaggaag ttttcgagtt
      181 tgatttacca ttaaagggcg agctgattaa cggagtttat gacggcattc aaataaaata
      241 ttgttacgcc tgcggtcgag acagcgcgtc aaaacccgaa gaaattttaa ttaatcgttt
      301 atcaattcct gacgacacgg acattttcag agtggctaat tttacgacga ttattttagc
      361 caccgaaaga tttgtcgaga cggtcacgaa tctgaatatt aagggcgctg tttttgaaga
      421 ggtaaaaatt gtctgacaaa atctttacct ctccgatcca aattacacta atcgattttc
      481 ttgcccttga tttccctgcc gctcggaata acgatggtta cgccggcgat ttttccggcg
      541 gcgtcgcgtt caaaggtgac ggtaacgctt gccgactgcg cgacgaattt gtcttttgtc
      601 gatgcgtcgg gcgagagttc gaggcgctcg ccgcccgcgt tgccgattag cttttcgcct
      661 tcctgttgga aagttacagc gcccatcggc gtgtcgaatt taccggcgaa atcggcgaat
      721 ttgccgccga cggtcggctt gatttcgacg ggcgcgggaa acgccgagcc cggtttgggc
      781 aaaaccagcg agccggtcga gttggtctgc agcgtatcaa aaggatgaat cggaaaagcc
      841 gggcgtttcg gctggacgac cggatttttt tgaatctgcg ccagcgccac ttcgaccgct
      901 ttttcaagct gcgaatcttt ccccgccatc gaatcctgcg gcgtaatttc gacgtcgaaa
      961 tccggcacga cgccgtagtt ttccacttcc cacgtcccgt tcggattata cgccgcgcgg
     1021 ttcggcagct gcacgccgcc gttgtcaatc aggcgcggcg tgaaaaagta aggtccgatg
     1081 ccagcgccgt aagtgcgctt gccgacaatc tgcccgacct tgccgagctt gaacataaac
     1141 gcgccggttt ccgccgccga gccgttaaac tcgttagtga ttaagacctt gacgggcggc
     1201 gcgggattga cgggcgtcgg gatgtcgtcg ccgccgcgaa acatataata ataaagcggt
     1261 tttcgaagca tccattcgat caggtaatcg ggcgtgattc cgccgccgtt atagcgctgg
     1321 tcgatgataa cgcccgcttt gtcggcgtag cctgaaagcc cgcgaatggc gttgctgatt
     1381 ccgtcgggat tgtagccttc gataaaaata tatccgagct tgccgcccga aagcctttcg
     1441 accgttttgc gattgtcttc cgcccaattg gcgcggcgca ggcggttttc gccgcttgcc
     1501 ggatagaccg taaaagcgcg cgcgtttgag ccgttcgcgt tattgctgac tgtaatgcgc
     1561 gtcggtctgc cggcggcgtt ttcaaaatag ctcagaatat ttttgctcgc ttcgattttc
     1621 tgcccgttga cctccaaaag aaaatcgcct tcgcggacat cgacgcccgg ctggtcgagc
     1681 ggcgcgctga acgagccgtt ggcgacggta aaaggcgtcg agcgataaat tttcttaaag
     1741 cggtaatgcc cgtcggcgat ttcataatcc gcgccgagca agccgatgcg atttccgccg
     1801 ccttgcggcg gcagcgaatc accgccgccg acgccgagat ggctgacgga gacgtgtccg
     1861 agcatctgcc gcatcagccc gtttaaatcg ccgcgccgcg tcacggtcgg caggtatgcg
     1921 gaatattcgc gctccagcaa agcgagattc tgcccgtgat aattcgggtc gtaaaaccag
     1981 tcgcgcataa tccgcatcga ctcgtgaaac atctgtcgcc attcttccga aggattaacg
     2041 cgcacttcca tcttgttgaa atcctgtctg ccgtcttcgg gtttagcggg cgtttccgcg
     2101 ccggtcagaa aataatcgcg tccctttcga tacaaaatgc ggctgccgtc gcgcgtgatg
     2161 tcgacggcgt ttatctcgtc cacgattttt tccattctgc cgcctttcgc caaatcgaaa
     2221 agatagacgg cacgcgattg cgacggctcg gtaaaatcgc cgggcgcttt cgaccactcg
     2281 ctgacgacca ggtgcagttt gcccgctttg cccgccgcga gctgcgaata atcgcgctgc
     2341 ggcagcggca ggttaacaaa acgcgccgac gcattgtcaa aatcaatcct gacctgcgat
     2401 acggcttcat taatttttgc gtccggattc ggctgcccgt taggcaaaag cggcgacggc
     2461 gcatctttcg acaagataaa tgcgtgcacg cggcgcacga ttagcggatt ggctaaaacc
     2521 ccgttcagaa cgccccagtt gaattcgctc gttccggcgt tggcgctgga gaggaaatag
     2581 agatatttgc cgttcgggtc gaaaacggga agctgcgtgt gcgttacgcc gtcggtgatt
     2641 tggacgtttt tcttctgcgc caaatcgcga atgtaaacgg ttccggcgcg attgcgcagc
     2701 cgtttcgcgt aagcgagata tcgcgaatcg ggcgaaaaat tcggcgtcca gccttcctgc
     2761 gccgaataaa cggacgtgtc gattttttct accgttccct tctcggcgtc agccgtccat
     2821 aaattcaatt gacggtcgct gaaagcgatt ttttttgaat cgggcgacca gattgtttcc
     2881 cagtaaaacg aaggcttcgg ctcgaccgcg atttttttga ccgaatcgtt ttccagcgag
     2941 cgaacgtgaa gcgcgtattc tccgctctcg tcggaaaaat aagcgactga ttttccgtcc
     3001 ggcgagatta ccggataacg ctcggcgacg ccggaagagt tcgtcagatt tttcgaatta
     3061 ccgtttgccg tatcgaataa taaaacttcg ccgcgcgcgc cgaaaactat ccggtcgccc
     3121 gaatccgacg gtagaatctg ttcgagaaaa cgaatcgcct gcacgtttcg cggtcttagc
     3181 tcggcggtgt cgggcgcgac cgaaacgtca atggcgcgcg tctgattcgt gttcaaatcg
     3241 agcaggtgaa gccgcccgcc gtgcacgaaa caaatcgcgt cggtcgtcgc cgcagccgcg
     3301 cggacgccga aatctttaaa attcgtcaac tgtctgacct gcctggtttt gcggtcgtgc
     3361 gcgaaaagat taaaaattcc cgtgcggtcg ctggtgaaat aaattttatc gccgacccat
     3421 tgcgggctgc ggtcgttgta attcgggttc ggcaattttt cgagcgcgcc cgtttttaaa
     3481 tcggtaatcc aaatcggcgc gatcgcgccg ccgcgatagt aacgccattc gccgaaaccg
     3541 agccgcgggt tgaaagcgat aaaattaccg tcgggcgaaa acacgccttg atacgcctgc
     3601 tgcatcggca gcggcgtcgc gagcgttccg ttttcgattg ccattttgta aagccgcgtg
     3661 acgccttcct ggtcacgcgt cgtttcaaaa atgacttccc tgccgtccgg cgtccaggca
     3721 gtcactaaat cgtcttccgg cataatcgtc acgcgccgcg cttcgccgcg accgtcggca
     3781 gaaataacga aaacgtccca gtcactgccg ttcaggcgcg aaaaagcgat ttgctttccg
     3841 tcgggcgaaa aaatcggatt cgtttcttcg ttcggcgtgt tggtcaaacg ccgcgccgcg
     3901 ccgccgcttc tttcgacgag ccagattttt ccggcgtagg taaaggcgac gtgagtttga
     3961 ttgacggcga cgcg
//
LOCUS       DASWNE010000718         7529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_516694, whole genome shotgun sequence.
ACCESSION   DASWNE010000718 DASWNE010000000
VERSION     DASWNE010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7529
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516694"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1673
                     /locus_tag="VGB68_14045"
     CDS             <1..1673
                     /locus_tag="VGB68_14045"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0050411.1"
                     /translation="ADARSLEAAAPMILKTFCDHLQASISCLWLPEESGAELSCIYLE
                     TSKDVGDVSAFIAETRRRRLARGVGLPGHVWAKNAPVWLPDTINEERFPRAAFAASVG
                     LHSAVGFPIKIGRDFYGVIEFFTRQPLLHDQALINMLDAIGSEIGQFIRRKRVEAERE
                     SLLVREKHLREQAEAASRLKDEFLATVSHELRTPLNAILGWGQILQAGKLEGEERDNA
                     LETIYRNAKSQVQLIDDLLDTSRLITGNLRLNLSPAPVVPLIEAAIDVVLPAAEAKGV
                     TLSAVYNSDNETITCDQQRLQQMIWNLLTNGIKFTPPGGEVEIRLEKSSDKVQIIVSD
                     TGQGIAPEFLPFVFDRFRQEDSSSTRMHNGLGLGLSIVRHLAELHGGSVQAESDGIDK
                     GASFIITLPRSMSVNAPAEPVQEEANGHKPHEIAEPELKGVRVLIVDDDVDTCEMLTF
                     ALNQWGAEAQASGSVSEAFTSIAEWQPDVLLTDINMPGEDGYALINRLRSLNNGNGAD
                     IPVIALTAMARPEDSEQALLAGFRLHLSKPVDIQELGEAIISLTKKSA"
     gene            2253..3482
                     /gene="hemA"
                     /locus_tag="VGB68_14050"
     CDS             2253..3482
                     /gene="hemA"
                     /locus_tag="VGB68_14050"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEY0050412.1"
                     /translation="MLFAFGLNHKTAPIEVREKLYIHESEIPALLARLRETLSECVIL
                     STCNRTEIYGVCEADADLDFYKELVIKFKNVEGIVGKEHFFTFISCAACQQLFKVATS
                     IDSKIIGDTQILQQLRNAYTLAKDNASTGKILNQLSQRAFKIGKKTYTETSIHKGAIS
                     ISLAAVELAIETFNSLKDKSVLIIGAGETAKLTAECLIKRQVGKIFITNRTRAHAAEL
                     LANLHKNHHFESEIIDFDDFKNYLNQTDIVISSTGSPVPILGAEDFNRQINKILLIDI
                     AVPRDIEPSVAENAAVVLKNIDDLHSIVDRNFERRMRDLPHVKKLISKEMSDFLMWYY
                     SLPLMPVFQKTRTKPDKSTVAEILEIKQFLIRNVSELHKLAMRNNGSVKDDLKNHVEL
                     IQKLQTMKNASFEVSDA"
     gene            3475..4431
                     /gene="hemC"
                     /locus_tag="VGB68_14055"
     CDS             3475..4431
                     /gene="hemC"
                     /locus_tag="VGB68_14055"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEY0050413.1"
                     /translation="MRKNNLIIGSRGSELALWQTNFVRSRLEEFFPALNLEIKIIKTT
                     GDKLLDVALAKIGDKGLFTRQIETALLGGEIDLAVHSLKDLQTVQPEGLIIGAVSERE
                     TPNDVLISKNAASIDDLPKGAKVATGSLRRKSQLLNYRPDLQIFEIRGNVPTRLKKFE
                     ESDLDAMILAFAGIRRLGLDAYIKQIIPFEIMLPAVSQGAMGVEIRSGDAELKELLRP
                     LNDENTQACVAAERSFLRALEGGCQVPIGAYAFLKDDKIYLEGMAGDLNGSVNLRDSI
                     SGEKENAARLGWRLAQNLIGKGADKLLERTRETVEKTKETVI"
     gene            4428..5183
                     /locus_tag="VGB68_14060"
     CDS             4428..5183
                     /locus_tag="VGB68_14060"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEY0050414.1"
                     /translation="MKRAPVILVVRQFDKFSSRLKDDGFEIINFPAIETVPVEDSGNL
                     DEKPKTLEKYDGLFFTSPKAAEIFLQRKTGSGFRGKVYVLGKRTKLLFENTNFEIVFC
                     ENANTARELINSFGESEFAGKKFLFLRGDKSLRAIPELLETIAEIDEIIVYRTIENAF
                     DKILIAESGEKLRRGEIDWICFFSPSAVESFVKTFGDLLPDEIKIAAIGTTTAKKAAA
                     ENLNVEFVSPKASAEDFALGLIEYLKEDSGFRI"
     gene            5339..6379
                     /gene="hemE"
                     /locus_tag="VGB68_14065"
     CDS             5339..6379
                     /gene="hemE"
                     /locus_tag="VGB68_14065"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEY0050415.1"
                     /translation="MSENSLFIRACKRQATERTPVWIMRQAGRYLPEYRAIRRKSDFL
                     TLCKTPELASEVTVQPIDIIGVDAAILFSDILVIPEAMGMRLEIIESKGPVFENPLRS
                     RDEINSLETEGVTERLSYVMDAIKLTKEKLNNRVPLIGFSGAPWTLATYMVEGKGSKN
                     FDVVKSFIYNEPKAAHALLQKLADAVVDYLNAKIRAGCDAVQIFDTWAGILSPTDFEE
                     FSLKYISYICERLETNGAPVIVFAKGVASVRKLADLKCDVLGIDWTKDIGDIFGEVGD
                     KKAIQGNLDPCVLFAPKEKIREETAKILRKFGRGAGHVFNLGHGILPTTPVENARYLV
                     NCVKELSIEYHE"
     gene            6583..>7529
                     /gene="hemN"
                     /locus_tag="VGB68_14070"
     CDS             6583..>7529
                     /gene="hemN"
                     /locus_tag="VGB68_14070"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEY0050416.1"
                     /translation="MSNFKEIDIDVLKKFNQPGPRYTSYPTAPVFSPDFTAADFKNEI
                     TDTNSASSSSEISLYFHFPFCESLCYFCGCNMMVSRNRQMIREYNEYLKKEIEQIAPL
                     VSPKRKIAQMHWGGGTPSYLFPEEILDVGSFIKSKFDFAADIEASVEIDPRGLTREHV
                     RAFSESGFNRLSMGVQDFNPQVQKAINRIQSEEITRAAIDWARELGFESINLDLIYGL
                     PFQTLDSFRETVEKIIDISPNRIAVFNYAHVPWLKKHQNVIPSEAIPAPDERLGIFKM
                     TIEKLMNAGYEYIGLDHFAKPSDELAVAQKNNTLYRNFQG"
BASE COUNT         1994 a         1833 c         1924 g         1778 t
ORIGIN      
        1 tcgccgatgc gcgcagcttg gaagccgccg cgccgatgat tctaaaaact ttctgcgacc
       61 atctgcaggc gagcatcagc tgcctctggc tgccggagga atcgggcgcc gagctctcct
      121 gcatttatct tgaaacatcg aaagacgtcg gagatgtttc ggcgtttatc gccgagacgc
      181 gccgccggag gctggcacgc ggcgtcgggc tgccgggaca cgtctgggcg aaaaacgcgc
      241 cggtttggct gcccgacaca attaatgagg aaagatttcc gcgcgccgcc tttgccgcgt
      301 cggtcggatt gcacagcgcc gtcggctttc ccatcaaaat cggcagggat ttttacggag
      361 tcatcgaatt tttcacgcgc cagcccttgc tccacgacca ggctttaatc aatatgctgg
      421 acgccatcgg cagcgaaatc ggtcaattta tccggcgcaa acgcgtcgaa gccgagcgcg
      481 aaagtctgct ggtgcgcgag aaacacctgc gcgagcaggc tgaagcggcg agccgtttaa
      541 aagacgagtt tctcgccacg gtctcgcacg agctgcgcac gccgctcaac gccattctgg
      601 gctggggaca aattctgcaa gccggaaagc tggagggcga agagcgcgat aacgcgctgg
      661 agacgattta ccgcaacgcc aaatcgcagg tgcagttaat cgacgatttg ctcgacactt
      721 cgcgcctgat taccggcaat ctgcgcctga acctgtcgcc ggcgccggtc gttccgctta
      781 tcgaagcggc gattgacgtc gttcttccgg cggcggaagc caaaggcgtg acgctctcag
      841 ccgtctataa ttccgataac gaaacgataa cctgcgacca gcaaagattg cagcagatga
      901 tttggaatct gctcaccaac ggcattaagt ttacgccgcc cggcggcgaa gtcgagattc
      961 gtctcgaaaa aagcagcgat aaagttcaga ttatcgtcag cgacaccgga cagggcatcg
     1021 cgcccgaatt tctgccattc gtgttcgacc gtttccggca ggaagacagc tccagcacca
     1081 ggatgcacaa cggtctggga ttaggcttgt caatcgtgcg gcatctggcg gagctgcacg
     1141 gcggttcggt tcaggcggaa agcgacggaa ttgacaaggg cgcgtctttt atcattacgc
     1201 ttccccgctc gatgtcggtc aacgcgcccg ccgagccggt tcaggaagaa gcaaacgggc
     1261 ataaaccgca cgaaatcgcc gagcccgagc tgaaaggcgt gcgcgtgttg attgtcgatg
     1321 acgatgtcga tacctgcgag atgctgactt tcgccctgaa tcagtggggc gcggaagcac
     1381 aggcgtccgg ctcggtttcc gaagctttca cttcgattgc cgaatggcag cccgatgttt
     1441 tgttgaccga tattaatatg ccgggcgagg acggttacgc gctgataaac aggctccgct
     1501 ctttgaacaa cggaaacggc gccgatattc cggtcatcgc gctgacggcg atggcgcgtc
     1561 ccgaagacag cgagcaggct cttttagccg gttttcggct tcacctgtca aaacccgtcg
     1621 atattcagga attaggcgaa gctattatca gcctgactaa aaaatctgcg tgagcgccga
     1681 gccgaaattt cgattgccgt tgagagccgg taaaccataa gcatcttctt tagcttattt
     1741 tttgtcggtg cagaatcgtt tttgcccgtg ccggagcaca ttttacgcgc gcgacgcata
     1801 ttttctccgt gcaaagcgca gtttccctgt gcaaactgta ttttgtgcgc gcaagacgat
     1861 ttttcgttgt gcagagcagt tattccgtct gcaacgaatt ttcaaggtaa acaaagcaac
     1921 cggctttgcc gcgtgttcaa cccgacaatt aatcactttt tcttatctgc gcaaatctgc
     1981 ggcggtctcg tttttcttga ttctccgcat tatttttttt cttttcacgc caatcgcggg
     2041 cggatattct caagccggtt tcgtcgcccg gacaaatcac ctgcatttat aaattgttct
     2101 tatctagtat tttttccgaa tttatgagga ctactcttta taacgcgggc tttttcgata
     2161 taatgaagtt tgtttaccgg aaaattaagc ttacgaatca acgaacaaga ggttttagaa
     2221 aaattcgctt ggaaaatatt gccgaccaaa aaatgctgtt tgcgtttgga ctcaaccaca
     2281 aaacagcgcc gatagaagtc agagaaaagc tctacattca cgaatcggaa attcccgcgc
     2341 tgctcgcgcg gctgcgggaa actctgtcgg aatgcgtgat tttatcgacc tgcaaccgca
     2401 cggaaattta cggcgtttgc gaggctgacg ctgatttgga tttttacaaa gaactcgtta
     2461 tcaaattcaa aaacgtcgag ggcattgtcg gcaaagaaca tttttttaca tttatttcgt
     2521 gcgccgcctg ccagcagctt ttcaaagtgg cgaccagcat cgattcgaaa atcatcggcg
     2581 acacgcaaat cttgcagcag ttaagaaacg cttatacgct cgccaaggac aacgcttcga
     2641 ccggaaagat tctcaaccag ctttcgcagc gggcttttaa aatcggcaaa aaaacttaca
     2701 ccgaaacctc gatacacaaa ggcgcgattt ccatcagcct ggcggcggtc gagctggcga
     2761 tcgagacgtt taattcgcta aaagacaaat cggttttgat aatcggcgcg ggcgagaccg
     2821 ccaaactgac cgccgaatgc ctgataaaaa gacaggtcgg gaaaattttt atcaccaatc
     2881 ggacgcgcgc tcacgccgcg gagcttctgg cgaatctaca caaaaatcat catttcgaaa
     2941 gcgaaattat cgacttcgac gatttcaaaa attatctgaa ccaaactgac atcgtcatca
     3001 gctcgaccgg ctcgcccgtg ccgattctgg gcgcggaaga tttcaaccgt caaatcaata
     3061 aaattctcct gatcgacatt gccgttccgc gcgacatcga gccgtcggtc gccgaaaacg
     3121 cagcggtcgt tttgaaaaat atcgacgacc tgcactcgat tgtcgaccgc aatttcgaaa
     3181 ggcgaatgcg cgacttgccg cacgtcaaaa agttgatttc aaaggaaatg agcgattttc
     3241 tgatgtggta ttactcgctg cccctgatgc cggtttttca gaaaacgcgc accaagcccg
     3301 ataaatcgac ggtcgcggaa atcctcgaaa tcaaacagtt tctcatccgc aacgtttccg
     3361 aattgcacaa gctggcgatg cggaataacg gcagcgtaaa ggacgatttg aaaaatcacg
     3421 tcgaactaat ccaaaaactc cagacgatga aaaacgcatc tttcgaggtt tcagatgcgt
     3481 aaaaacaatc tgattatcgg ctcgcgcgga agcgagctcg cgctctggca aacaaatttt
     3541 gtccggtcgc gtcttgaaga attttttccc gcgctcaatc tcgaaattaa aatcatcaaa
     3601 accacgggcg acaagcttct cgacgtcgcg ctggcgaaaa tcggcgacaa aggcttattt
     3661 acgcggcaga tcgaaaccgc gcttctaggc ggcgaaatcg acctcgccgt tcacagtctg
     3721 aaagatttgc aaaccgttca gcccgaaggc ttaatcatcg gcgcggtctc ggaaagagaa
     3781 acgccgaacg atgttttgat ttccaaaaac gccgcttcga ttgacgattt gccgaaaggc
     3841 gcgaaagtgg cgaccggcag cctgcgccgc aaaagccagc ttttaaatta ccgtcccgat
     3901 ttgcagattt ttgaaattcg cggaaacgtc ccgacgcgcc tgaaaaaatt tgaagaatcg
     3961 gatttggacg cgatgattct cgcctttgcg ggcatacgcc ggctcggctt ggacgcgtat
     4021 atcaaacaaa ttattccttt tgaaattatg cttccggcgg tcagccaggg cgcgatgggc
     4081 gttgaaatca ggagcggcga cgcggaatta aaagaactgc tgcgaccgct gaatgacgaa
     4141 aacacgcaag cttgcgtcgc ggcggaaaga agctttctgc gcgcgctcga aggcggctgc
     4201 caggttccga tcggcgcgta tgcttttttg aaagatgata aaatttatct ggaaggaatg
     4261 gcgggcgacc tgaacggaag cgtaaatctg cgcgattcga tttcgggcga aaaagaaaac
     4321 gccgctcgat tgggctggcg attggcgcaa aatttaatcg gcaaaggcgc ggataaatta
     4381 ctggagcgaa cgcgcgaaac tgttgaaaaa acaaaggaaa cggttatttg aaacgcgcgc
     4441 cggttatttt agtcgtccgt caattcgata agttcagctc gcggctgaaa gacgacggat
     4501 ttgaaattat aaattttccg gcaatcgaaa ctgtgccggt tgaagattcc ggcaatttgg
     4561 acgaaaagcc gaaaaccctc gaaaaatacg acggtttatt tttcacgagc ccgaaagcgg
     4621 cggaaatttt tctgcaaaga aaaaccggaa gcggctttcg cggaaaagtt tacgttttgg
     4681 gcaagaggac gaaattatta tttgaaaata cgaattttga aattgttttt tgcgaaaatg
     4741 cgaatacggc gcgagagctt ataaattctt tcggcgaaag cgaatttgcc ggtaaaaaat
     4801 ttctttttct gcgcggcgac aaaagcctgc gggcgattcc cgagcttttg gaaacaatcg
     4861 ccgagattga cgagattatt gtttaccgaa cgattgaaaa cgcgtttgat aaaatcttaa
     4921 tcgccgaatc gggcgaaaaa ttgcggcgcg gcgaaatcga ttggatttgc tttttcagcc
     4981 cttcggcagt tgagagtttt gtaaaaacgt tcggcgattt gttgccggac gaaataaaaa
     5041 tcgcggcaat cggcacgacg acggcgaaaa aagccgccgc cgaaaacttg aacgttgaat
     5101 tcgtttctcc gaaagcgagc gccgaagatt ttgctttggg attgattgaa tatttgaaag
     5161 aggattcagg attcaggatt taagattcaa gatttaagat tcaggattta agattcaaga
     5221 tttcagattt cagatttaga agtcagaagt caaaatataa tcctgaatcc tgaatcctga
     5281 atcttaaatc ctgaatcctg aatcctgaat ccagaatcct aattacattt aaattatttt
     5341 gagcgaaaat tcattattta tcagagcctg caaacggcag gcgacagagc gaacgccggt
     5401 ctggattatg cgccaagccg gtcgttatct gcccgaatat cgcgcgattc gccggaaaag
     5461 cgatttttta accttgtgca aaacgccgga actggcaagc gaagtgaccg ttcagccaat
     5521 cgatattatc ggcgtcgacg cggcgatttt gttttccgat attctcgtca ttcccgaagc
     5581 gatgggaatg cggctggaaa tcatcgaatc gaaaggtccg gttttcgaaa atccgctgag
     5641 aagccgcgac gagataaatt ctctcgaaac cgaaggcgtc accgagcggc tttcttatgt
     5701 aatggacgcg ataaagctga cgaaggaaaa attaaacaat cgcgttccgt taatcggttt
     5761 ttcgggcgcg ccctggactc tggcgactta tatggtcgaa ggcaaaggct cgaaaaattt
     5821 cgacgtcgtc aagagcttta tttacaacga gccgaaggcg gcgcacgcgc tcttgcaaaa
     5881 gctcgccgat gcggtcgtcg attatctgaa cgcgaaaatc cgcgccggct gcgacgccgt
     5941 gcagattttc gacacgtggg cgggaatttt atcgccgacg gatttcgagg aattttcgct
     6001 gaaatatata agctatattt gtgagcggct cgaaacaaac ggcgcgccgg tgattgtttt
     6061 cgccaaaggc gtcgcttccg tgcggaaact ggctgatttg aaatgcgacg ttctgggcat
     6121 cgactggaca aaagacatcg gcgacatttt tggggaagtc ggcgataaaa aagcgattca
     6181 gggaaatctc gacccgtgtg ttttgtttgc gccgaaagaa aaaatcagag aggaaaccgc
     6241 gaaaattctg cgaaaattcg ggcgcggcgc gggacacgta ttcaatctcg gacacggaat
     6301 tctgccgacg acgccggtcg aaaacgcgcg ttatctggtg aactgcgtca aggaattgag
     6361 catcgaatat catgaataag gaatcgcgca gacggcaaat gacacggatt aagaaaatga
     6421 aaccgcagat ttacacagat aaacgcgaat tttaacagga tacgcaggat aggcaggata
     6481 atttaaaact gagaacattg acctttcttt tttatcctgt ttatcctgtg tatcctgtat
     6541 gaatttctct atattctctg tgtcctctgt ggctgaagaa aaatgagtaa ttttaaagaa
     6601 atcgatattg atgtcctcaa aaagtttaac cagccgggac cgcgttatac ctcgtatccg
     6661 accgcgccgg ttttttcgcc cgattttacg gcggcggatt tcaaaaacga aataaccgac
     6721 accaattccg catcgagctc aagcgagatt tcgctttatt ttcatttccc tttctgcgaa
     6781 tcgctctgct atttctgcgg ctgcaatatg atggtttcac ggaaccggca aatgattcgc
     6841 gaatacaacg aatatctgaa aaaggaaatc gagcaaatcg cgccgctcgt ttccccgaaa
     6901 agaaaaatcg cgcagatgca ctggggcggc ggaacgcctt cgtatctttt tcccgaagaa
     6961 attctcgatg tcggaagctt tatcaaaagc aaattcgatt ttgccgccga tatcgaagcg
     7021 agcgtcgaga ttgacccgcg cgggctgacg cgcgagcacg ttcgagcctt tagcgaatcg
     7081 ggctttaatc gactgagtat gggcgtgcag gattttaatc cgcaggtgca aaaggcgata
     7141 aaccgcattc aatccgaaga aattacgcgc gcggcgattg attgggcgcg cgagctcgga
     7201 tttgaaagca tcaatctgga tttgatttac ggcttgccgt ttcaaacgct cgattctttc
     7261 cgcgaaacgg ttgaaaagat tatagatatt tcgccgaacc ggatcgccgt tttcaattac
     7321 gcgcacgttc cgtggctcaa aaagcatcaa aacgtcatcc cgtcggaagc catacccgcg
     7381 cccgacgagc ggctcggtat tttcaaaatg acgattgaaa aattgatgaa cgcgggctac
     7441 gaatacatcg gtctcgacca tttcgccaag ccttcggacg agctggcggt cgcgcagaaa
     7501 aacaacacgc tttaccgcaa ttttcaggg
//
LOCUS       DASWNE010000719         4960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_24796, whole genome shotgun sequence.
ACCESSION   DASWNE010000719 DASWNE010000000
VERSION     DASWNE010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24796"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VGB68_14075"
     CDS             complement(<1..340)
                     /locus_tag="VGB68_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050417.1"
                     /translation="MSNTIKNLHTAIRRYCMDRYSYWTEKYAELSSAGKDRAGYGYTD
                     EALCTFPRYNVLNAILFEIERYRPEEFATFDEAKRFFRLVAAEAENIFTRLPKGNLEQ
                     KAMGEERETLS"
     gene            541..939
                     /locus_tag="VGB68_14080"
     CDS             541..939
                     /locus_tag="VGB68_14080"
                     /inference="COORDINATES: protein motif:HMM:NF024886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HEY0050418.1"
                     /translation="MEQKPKLLEQVRAVAGARYLSHKTEDVYHNFIKRFILFHNKRHP
                     KEMGAKEITDFLTYLAVKENVSASTQNQALFALLFLYRDVLKIDLPRIEGVIRQRRAN
                     YADLHAPFEKQIFCQKSARHLSNCAACRFK"
     gene            complement(998..3361)
                     /locus_tag="VGB68_14085"
     CDS             complement(998..3361)
                     /locus_tag="VGB68_14085"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HEY0050419.1"
                     /translation="MATATKKAGTAKSKNINKQGQNDTTTPKAKKAAKNNDAKVLIKP
                     EGAIGDGGLLDHDATQKTAKSIKPEEPKKAARKNGKAATNAAARAKDYAGLDTQTLVG
                     LYRTIYTSRRVDDKEIQLKGQNKIFFQISGAGHEAMLVGAGLALKAGYDWFFPYYRDR
                     ALLLPLGMTPLEMLFSSVGAEADPNSHGRQMPSHWGHVKLNVPSQSSCTGTQALHAVG
                     AAEASYRASLVKELQDKITGFKGDEVVYMSVGDGTTSEGEWWEALNTACNLKLPVIFV
                     VEDNGYAISVPVEVNTAGGDISRLVAGFPNLYIQKCDGTNPIESYETFKRAAEYCRAR
                     KGPAFVHAKVIRPYSHSLSDDEKSYRPDEEREREAAIDPVKTFAEFLMTEGIASAEDL
                     EKLRKEVDAEINEATDVALATPQPAPESALRNVFSPDIDPTSKDFDTEEGAELSGNAG
                     TMVDLINRCLHEEMERDERIVIFGEDVADASREEYLERVKGKGGVFKVTANLQRKFGS
                     ARVFNSPLAEANIIGRAVGMSIRGLKPVVEIQFFDYIFPAFHQIRNEVAVTRWRSDGD
                     TKCPMVMRVPVGGYLKGGAVYHSQSGTTLFAHTPGLLIAYPSNALDANGLLRTSIRCD
                     DPVLFLEHKHLYRQIYNKSQYPSSEFLIPFGKAKVVREGSDVTIITYGALVQRSLEAA
                     KRLEKQGVSVELIDLRTLVPYDWERVAESVRKTSRAIVAHEDSVSYGYGAEIAARISD
                     ELFEYLDAPVRRVGATDTFVAYAPQVEDFILPQIEDVEKAVQELMKY"
     gene            complement(3559..3876)
                     /locus_tag="VGB68_14090"
     CDS             complement(3559..3876)
                     /locus_tag="VGB68_14090"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0050420.1"
                     /translation="MQNRIEVFRQMMESDPNNTMVLFGLANEYQKAEDWQKTIETLEN
                     YLAQADDEGAAYGMLARAYEKTGNREKARAAYEKGVEVSMKHGHPTMAQDYSATLDLD
                     YAD"
     gene            complement(4116..>4960)
                     /locus_tag="VGB68_14095"
     CDS             complement(4116..>4960)
                     /locus_tag="VGB68_14095"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0050421.1"
                     /translation="VNCYRGCIHACTYCFARRYHEFLGYGAGTDFETKIVAKINAPAV
                     LREELKRTREKIGQLEFSFTTDPYLPLEANYELTRKCLIICRDFRMPVGIITKSPLIT
                     RDLDVLTELDGMVFFSIPFPTIEKSKPFEPFVPVPEARFRAMETLAKAGVEVGIGIAP
                     IIPTYNDADIPILLEKARNAGATRAFINLIHFDSDSVEDYFVQKLKEKLPTKADKILN
                     ALRRERGGQLRHRNFAERGGKTEQWQTAVRLFDLHFRRLGFTKFRRIEKTEEKPAPEQ
                     PRLF"
BASE COUNT         1153 a         1286 c         1230 g         1291 t
ORIGIN      
        1 ttgaaagtgt ttcgcgttct tcacccattg ctttctgctc aagattacct ttcggcagtc
       61 gcgtaaatat attttcggct tcagcagcaa caagacgaaa aaagcgtttg gcttcatcaa
      121 acgtcgcaaa ttcttcagga cggtaacgct cgatttcaaa gagaattgcg ttcagcacat
      181 tgtaacgcgg aaaagtgcaa agtgcttcgt cggtgtagcc ataaccagcg cggtcttttc
      241 cagcagatga gagttcagca tatttctccg tccaatatga atatctgtcc atacaatatc
      301 ggcgaattgc agtgtgaaga tttttgatag tgttactcat aaagcattgc cgatgataaa
      361 aggaatacgg cgaacttatg attgtctgga atgattccag atagctgcca aaattcgtgt
      421 gttatcgcga accgctcttg gtaatacacg aaatttgtgt attatctgga atcattcggg
      481 ataataacct tatatacgga atgaaaataa actaattttc ccgcttttgc aataaaattt
      541 atggaacaga aaccaaaatt gttagagcag gtcagagccg ttgccggagc gagatatttg
      601 agtcataaga cggaagacgt ttatcataat tttatcaagc ggtttatttt gtttcacaat
      661 aagcggcatc cgaaggaaat gggcgcgaag gagattaccg atttcttgac gtatttggcg
      721 gtcaaggaga atgtttccgc ttcgacgcaa aatcaggcgt tgttcgcttt gctgtttctt
      781 tatcgtgacg ttttaaagat tgatttgccg cgtattgaag gcgtgattcg acaaagacgt
      841 gcgaactacg cagatttaca cgcacctttt gaaaaacaaa tcttttgtca aaagtccgct
      901 cgacatctga gtaattgtgc ggcttgtcgc ttcaaataaa aagcgcagga ttagctaaac
      961 cttgcgcttt ctaattttaa ttaaaaattt cgctctttta atatttcatc aattcctgga
     1021 cggctttttc cacgtcttca atctgcggca gaatgaaatc ttcgacctgc ggcgcgtagg
     1081 cgacaaacgt atcggtcgcg ccgacgcgcc ggacgggcgc gtcgagatat tcaaacaatt
     1141 cgtccgaaat gcgcgcggcg atttccgcgc cgtagccgta ggaaaccgaa tcttcgtgcg
     1201 cgacgatagc gcgcgaagtt ttcctgaccg attcggcgac gcgttcccaa tcgtatggaa
     1261 cgagcgtgcg caaatcaatc agctcgaccg aaacgccctg tttttccaga cgcttcgccg
     1321 cttccagact tctctggaca agcgcgccgt atgtgattat cgtgacgtcc gagccttctc
     1381 tgacgacttt cgccttgccg aacggaatca gaaattccga cgacggatac tgcgatttgt
     1441 tgtaaatctg ccggtagaga tgcttgtgtt ccaaaaacaa aaccgggtcg tcgcagcgaa
     1501 tcgaagttct caaaagcccg ttggcgtcca gcgcattcga cgggtaagcg atgagcaagc
     1561 cgggcgtgtg cgcgaaaagc gtcgttcccg attgcgagtg ataaaccgcg ccgcctttca
     1621 gataaccgcc tacgggaacg cgcatcacca tcgggcactt cgtgtcgccg tcagaacgcc
     1681 agcgcgtgac ggcgacttcg tttctgattt ggtggaacgc cgggaaaatg taatcgaaaa
     1741 actgaatctc gacgaccggc ttcagcccgc gaatcgacat tccgacggcg cgaccgatga
     1801 tgttggcttc cgccagaggc gaattaaaaa cacgcgccga gccgaatttt ctttgcaggt
     1861 tcgccgtcac tttgaacacg ccgcctttgc ctttgacgcg ctccagatat tcctcgcgcg
     1921 aggcgtccgc gacgtcttcg ccgaaaatca cgattcgctc gtcgcgctcc atttcttcgt
     1981 gcagacagcg gttaatcaaa tcgaccatcg ttcccgcgtt gccggaaagc tcggcgcctt
     2041 cttccgtgtc gaaatctttg gaagtcgggt cgatgtcggg cgaaaacacg tttcgtaagg
     2101 cgctttccgg cgcgggctgc ggcgtcgcca gcgcgacgtc ggtcgcctcg ttgatttcgg
     2161 cgtcaacctc tttgcgcagt ttttccaaat cttccgccga cgcgatgcct tcggtcatca
     2221 gaaattcggc gaaggttttg accgggtcga tcgccgcctc gcgctcgcgc tcttcgtcgg
     2281 gacgatacga tttttcgtcg tccgacaagc tgtgcgaata cgggcgaatc acttttgcgt
     2341 gaacgaaagc cggacctttg cgcgcgcgac agtattcggc ggcgcgttta aaggtttcgt
     2401 aagattcaat cgggttcgtg ccgtcgcatt tctggatgta aagattagga aaacccgcga
     2461 ccagcctgga aatatcgccg cccgccgtgt tgacttcgac cggaacgctg atggcgtagc
     2521 cgttatcttc aacgacgaaa attacgggca atttcagatt gcaagccgta ttgagcgctt
     2581 cccaccattc gccttcgctc gtcgtgccgt cgccgaccga catataaacc acttcgtcgc
     2641 ctttgaaacc cgtgattttg tcctgcaatt ccttgacgag cgacgcgcga tagctcgcct
     2701 cagccgcgcc gaccgcgtgc agagcctgcg ttcccgtgca ggaagactgc gacggaacat
     2761 ttaatttcac gtgtccccag tgcgagggca tctgtcgccc gtgcgagttc gggtcggctt
     2821 ccgcgccgac cgacgaaaaa agcatttcca gcggcgtcat tcccaatggc agaagcaaag
     2881 cgcggtcgcg gtaatacggg aaaaaccaat cgtagcccgc tttcaaagcc agccccgcgc
     2941 cgaccagcat cgcttcgtgt cccgcgccgc tgatttggaa aaagattttg ttttgaccct
     3001 tgagctgaat ttccttgtcg tcgacccgcc gcgaagtgta aatcgtgcgg taaagaccga
     3061 ccagcgtttg cgtgtccaga ccggcgtaat ctttcgcacg cgcggcggca ttggtcgccg
     3121 ctttgccgtt ttttcgcgcc gcttttttcg gttcttccgg tttgattgac ttggcggttt
     3181 tctgcgtcgc gtcgtggtcg agcagaccgc cgtcgccgat ggcgccttcg ggtttgatta
     3241 aaactttcgc gtcgttgttt ttcgcggctt ttttagcttt tggcgtagtg gtgtcgtttt
     3301 gcccttgttt attaatgttt ttagattttg ccgtgccggc tttcttggtt gctgttgcca
     3361 tttttataaa ttcccgattg aataaagatt tattatagac gaatcaacta ttttaaccgc
     3421 ttacagaggt aattttccag cagcggcgcg ctgtttctca ggaaacgccg aacgccgaac
     3481 ccggaacggt gaataaaaaa agaaacgtgg aaactgcaat tcacgcgttt cgcgcgttcc
     3541 gggttcagcg ttttaaaact aatcggcgta atccaaatcg agcgtcgcgc tataatcctg
     3601 cgccatcgtc ggatgaccgt gtttcattga aacctcgacg cctttttcat aagcggcgcg
     3661 cgctttttcg cggtttccgg ttttttcgta agcgcgggcg agcattccgt aagccgcgcc
     3721 ttcgtcgtcg gcttgcgcca gataattttc gagcgtctcg attgtttttt gccagtcttc
     3781 agccttttga tattcgtttg ccaatccgaa caaaaccatc gtattattcg ggtcggactc
     3841 catcatctgt cggaaaactt cgattcggtt ttgcataaaa caaaattaac atttgcgccg
     3901 aagaaagaaa aatgccgccc gcgcaaccgc tgctttcttc aaagcaacct attttataaa
     3961 cgaatacgcg cgggaaatct atgaaaaaac cgacgcttct tttaatattt ccgctcgccg
     4021 aaagcgaaag ggaattggag cgaaaggaac cggaaaaaca aaaaaactct tagctcggca
     4081 ataaaaagat ttattaattt tccaacggta aaattttaga aaagcctcgg ctgctcaggc
     4141 gcgggctttt cttccgtttt ctcgattcgt cgaaatttag taaaacccag acgcctgaag
     4201 tgcaaatcaa acagcctgac cgccgtctgc cattgctcgg tcttgccgcc tctttccgca
     4261 aaattgcgat gccgcagttg cccgccgcgc tcgcgccgca gcgcatttaa aattttatcg
     4321 gctttggtcg gcagcttttc tttcaatttc tgcacgaaat aatcttcgac cgaatcgctg
     4381 tcaaaatgaa tcaggttaat aaacgcccgc gtcgcgcccg catttcgagc cttttccaat
     4441 aaaatcggaa tgtccgcgtc gttataagtc ggaatgatcg gcgcgatgcc gatgccgacc
     4501 tcgacgcccg ctttcgccag cgtttccatc gcgcggaaac gcgcttccgg cacgggcaca
     4561 aaaggttcga aaggcttcga tttttcgatg gtcggaaacg gaatcgaaaa aaaaaccatt
     4621 ccgtcaagct cggtcaaaac gtctaaatcg cgcgtaatca gaggcgattt ggtaatgatt
     4681 ccgaccggca tccgaaagtc gcggcagatt attaaacatt tccgcgtcag ctcgtaattg
     4741 gcttccagag gcaggtaagg gtcggtcgta aacgaaaatt cgagctgccc gattttttcg
     4801 cgcgttcgtt tgagttcttc gcgcaaaact gccggcgcgt ttattttggc gacgattttc
     4861 gtttcgaaat ccgtgcccgc gccgtaacct aaaaattcgt gataccgccg cgcgaaacaa
     4921 tacgtgcagg cgtggatgca gccgcgataa cagttgaccg
//
LOCUS       DASWNE010000720         5234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_375750, whole genome shotgun sequence.
ACCESSION   DASWNE010000720 DASWNE010000000
VERSION     DASWNE010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5234
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375750"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..352)
                     /locus_tag="VGB68_14100"
     CDS             complement(221..352)
                     /locus_tag="VGB68_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050422.1"
                     /translation="MSGRDVSVCDEHDLQPVNKAFTPDRRLPSPATARASNYRFHRR"
     gene            390..2906
                     /locus_tag="VGB68_14105"
     CDS             390..2906
                     /locus_tag="VGB68_14105"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0050423.1"
                     /translation="MKVGQMLGSFKLLEVLGTGGQGTVFKALDTKLDRLVAIKILTPD
                     LKWRKKNLARFEREARLASSLDHPNICTIYGFYEDESFTYIVMPYIEGQNLQQLTLDG
                     RPLELRSALSITVQVADALVAAHAKGVIHRDIKAGNVIVTETGLAKVLDFGLAKLLEP
                     AAGADKSIAENPEEAHLTVAGIPYGTPSSSAPEMALGEPADHRADIFSTGVVLYQLLT
                     GRFPFLGKTVAEVRAKVVNQTPRSISEARGADLPVPPGLQEIADRALAKNPEDRFPTA
                     EEMRDALRAVLREIEPDEAALSYPVIPQTTAPRSARQKQQSAWSKRAIIFGSIALLLF
                     AAALSAYFLFPRPKHELESIAVLPFVIATGNEETEYLAEGVTESLIGSLAEIPNLRVR
                     SRDAVYRYRNSNQNTRDIGRALNVHVVLSGRIRQTGDEILINVELIDAKDDTHLWGSQ
                     YQGKLSEIVGLQREIVRDVAENLERPMSEAEERTLVKNYKTNSDAYQFYLQGRYFWNK
                     RRPDTLFKSIEYFERAVAADPDYAPAYAGLADSYSLLNVYNVSPATDFNTKARDAAVK
                     ALQIDPNLAEAYASLGTVNFRHEWNWREAEDNFERSIALDADYAPAHQWFAGLLSAEG
                     KHDAALPEIQKALELDPFSLSINADYGKYLYLARRFDEAAAAYRKTLELEPNYARAHL
                     ELGLVLAQQKQYEAAIREINRSAALSPQRGASLIENKPNETNETATLETSLSVNGNVR
                     AIAALGYVYGASGQRAQALEQIRQLQKLAQNRYVSPYYLAVVYAGLKEKDKALDELEK
                     SLEKRFNALVFLRVEPIFNDLQNERRFRKITETMDLPANR"
     gene            complement(2932..4389)
                     /locus_tag="VGB68_14110"
     CDS             complement(2932..4389)
                     /locus_tag="VGB68_14110"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY0050424.1"
                     /translation="MHISENRILKSYLSIVFIALGCTALYGQNIDEAIDKQIDRLFSS
                     YNSTTPGVAVAVVKDGKIIFKKGYGTANLEYDIPVTTKTVFQIASVSKQFTAFSIYLL
                     EKQGKISLEDDVRKYVPELPDAGKIIKIKHLLAHTSGIKDQAALLTLAGWRMDDVTTT
                     QQILKLISRQKELNFEPGSRYLYSNSGYTLLAEIVKRVSGASFADFTRKNIFEPLGMI
                     DTQFYDDYERTVKNRADSYELENGAYKKKSLSNSAAGASNLYTTVEDLAKWVSNFENP
                     KVGDAELIKRFNEASLLDNGDRVIWSISDGEPGYHAKGQIHWNYRGLHLMSHGGHAAA
                     FRSFLGRFPDQRLAVIALSNDEHYQNFNTSIRIAEFYLKDDLKPNQTANAPAALNKTA
                     PKPNADLKDFEGRFYNDELETAYSVKAAGGKLIFSHIRHADIELTDGGKDKFFGRIEF
                     PVEIEFVRAGNGAVTGFLISNFGAKNMKFDKTVGK"
     gene            complement(4740..4813)
                     /locus_tag="VGB68_14115"
     tRNA            complement(4740..4813)
                     /locus_tag="VGB68_14115"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4777..4779),aa:Arg,seq:cct)
     gene            complement(4913..>5234)
                     /locus_tag="VGB68_14120"
     CDS             complement(4913..>5234)
                     /locus_tag="VGB68_14120"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0106130 - purine phosphoribosyltransferase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="purine phosphoribosyltransferase family protein"
                     /protein_id="HEY0050425.1"
                     /translation="TAGSFFGLRTGTKPTKLPAEKVSVSYDLEYGQDTLEMHRDAVGE
                     GHRVLIVDDLLATGGTAKAVVDLVEGLGGKIVGLSFVVELNFLNGRAKFDGYNVSSLL
                     RYDV"
BASE COUNT         1302 a         1354 c         1314 g         1264 t
ORIGIN      
        1 cgaaacgcgc gtcgtcgccg tcgcccgcgg cttgagcaac aaggaaatcg ctcaggaatt
       61 caatatcagc gcgcgcacgg tcgaacacca tatccgaaat attttagcga aaaagaattt
      121 ctccaaccgc gtcgaaatcg cgctttacgt gagaaaccaa accgcgtcat aataacgcgc
      181 ctgacgtttt cgcgacggca agcgggcgcg aaagatttaa ttatcgacga tggaagcgat
      241 aattggaggc gcgagccgtc gcaggcgacg gcagccggcg gtcgggcgtg aaggctttgt
      301 ttacgggctg caaatcgtgt tcgtcgcaga cggaaacgtc gcgcccggac acgtctctaa
      361 cccgtcaatt aaaccgctga aaaactctta tgaaagtcgg acagatgctc ggcagcttca
      421 aacttctgga agtcctcggc acgggcgggc aaggaaccgt ctttaaagct ttggacacaa
      481 aactcgaccg cctcgtcgca atcaagattc tgacgcccga tttgaaatgg cgaaagaaga
      541 atctggcgcg tttcgagcgc gaagcgagac tcgcctcctc gctcgaccat ccgaacatct
      601 gcacgattta cggtttttat gaagacgaaa gcttcaccta tatcgtgatg ccctacattg
      661 aaggacaaaa cctgcaacag cttactctgg acgggcgtcc cctggagctg agaagcgcgc
      721 tctcgattac cgttcaagtc gccgatgcgc tcgtcgccgc tcacgccaaa ggagtcattc
      781 accgcgacat caaagccgga aacgtcatcg tgacggaaac cgggctcgct aaagtgctgg
      841 atttcggttt agccaagctg ctggaacctg ccgcgggcgc ggataaatca atcgcggaaa
      901 atccggagga agctcatttg accgttgcgg gaattcccta cggtacgccg tcctcctccg
      961 cgccggaaat ggctctggga gagcctgccg accaccgcgc cgatattttt tcgaccggcg
     1021 tggtgcttta tcaactgctg accgggagat ttccgttttt aggcaaaacc gttgccgaag
     1081 tgcgcgccaa agtcgttaat caaacgcccc gcagtattag cgaggcgcgg ggcgcagacc
     1141 tgcccgtgcc tcccggctta caggaaattg ccgaccgggc tctggcgaaa aatcccgaag
     1201 accgttttcc gaccgccgag gagatgagag acgcgcttcg cgccgttttg cgtgaaatcg
     1261 agccggacga ggcagcttta agctatcccg ttattccgca aaccaccgcg ccgcgttccg
     1321 cgcgtcaaaa acaacagagc gcttggtcaa aacgcgcaat cattttcggc tccatcgccc
     1381 ttttgctttt cgcggcggct ttatcggcat attttctgtt tccgcgcccg aaacacgaac
     1441 tcgaatcaat cgccgtcctg ccgttcgtaa tagcgacggg aaatgaggaa accgagtatc
     1501 ttgccgaagg cgtcacggag agtttgattg gcagtctggc ggaaattccg aatctgcggg
     1561 ttcgctcgcg cgatgcggtt tatcgctacc ggaattcaaa ccagaatacc agagatatcg
     1621 ggcgcgcgct taatgtccac gtcgttttga gcggcagaat ccggcaaacc ggcgacgaga
     1681 ttcttatcaa cgtcgaattg attgacgcca aagatgatac gcatttatgg ggcagccagt
     1741 atcagggcaa gctgtcggaa atcgtcgggc tgcaaagaga aatcgtgcgc gatgtcgccg
     1801 aaaatttaga acgaccgatg agcgaagcgg aagagaggac gctggtcaaa aattacaaaa
     1861 ccaattccga cgcctaccaa ttttacttgc agggacgtta cttttggaac aagcgccgcc
     1921 ccgatactct gtttaaaagc atcgagtatt tcgagcgagc cgttgccgcc gaccccgatt
     1981 acgctccggc ttatgccggt ctggctgact cctattccct gctgaacgtt tataacgtca
     2041 gcccggcgac ggattttaat accaaagcca gagatgccgc cgtcaaggct ctgcagattg
     2101 acccgaatct ggctgaagcc tacgcatcgc tcggaacagt taattttcgc cacgaatgga
     2161 actggcgcga agcggaagac aatttcgagc gcagcatcgc gctcgatgcc gattatgcac
     2221 cggcgcatca atggtttgcc ggcttattga gcgccgaagg caaacacgac gcggcgctgc
     2281 cggaaattca aaaagctctg gagctcgacc cgttttcgct ttcgattaac gccgattacg
     2341 gaaaatatct ttatttagcc cggcgttttg acgaagccgc cgccgcttat cgaaaaactc
     2401 tcgaacttga gccgaattac gcgcgggcgc atctcgaact gggcttggtt ctggcgcagc
     2461 agaaacaata cgaagcggca attcgggaaa ttaaccggtc ggcggcttta agtccgcaac
     2521 ggggcgcgag cttaatcgaa aacaagccga atgaaacaaa cgaaacggca actctggaga
     2581 cttcgctttc ggtaaacggc aatgtcaggg caatcgcggc gctcggctat gtttacggcg
     2641 cgagcggtca acgcgcgcaa gccttggagc aaatccggca gctacaaaaa ctggcgcaga
     2701 atcgctatgt atcgccgtat tatctggcgg tcgtttatgc cggattaaaa gaaaaagaca
     2761 aggctctcga cgagcttgaa aaatcgcttg aaaaaagatt caacgcgctt gtttttcttc
     2821 gggtcgagcc catttttaat gatttacaaa atgaaagacg gttcaggaag attaccgaaa
     2881 caatggattt accggcaaat cggtaatcgg ttaataaatt tcatttaaat ttcatttccc
     2941 aactgtcttg tcaaacttca tatttttcgc gccgaagttt gagattagga atccggtgac
     3001 cgctccgttt ccggctctga caaattcgat ctcgacggga aattcaattc tgccgaaaaa
     3061 tttatctttt ccgccatcgg tcagctcgat gtccgcgtgc ctgatgtgcg agaaaatcag
     3121 cttgccgccc gccgctttaa ccgagtaagc agtttccagc tcatcgttat aaaatctgcc
     3181 ctcaaaatcc ttcaaatcag cgtttggctt cggggcggtc ttgttcagag cggcgggagc
     3241 attggcggtt tgattcggct tcaaatcatc tttcagataa aattcggcaa tcctgatact
     3301 ggtattaaag ttttggtaat gctcgtcgtt actcagtgcg atgacggcta gacgctggtc
     3361 gggaaagcga ccgagaaagg agcggaaagc cgccgcgtgc ccgccgtgac tcatcaaatg
     3421 cagacctctg taattccagt gaatttgccc tttggcatga tagccgggct cgccgtcgga
     3481 gatgctccaa atgacgcggt ctccattatc gagcagcgaa gcttcattga atctttttat
     3541 caattcggcg tctccgactt tcgggttctc gaaattcgaa acccattttg ccaaatcttc
     3601 gaccgtcgta taaagatttg atgcgccggc ggcggaatta ctcaagctct tctttttgta
     3661 agcgccgttt tccaattcgt atgagtcggc tctgtttttg acggttcttt cataatcgtc
     3721 ataaaactgc gtgtcaatca ttccgagcgg ctcgaaaata tttttccttg taaagtcggc
     3781 aaacgacgcg ccgctgacgc gcttgacgat ttccgccagc agcgtgtagc ccgaattgct
     3841 gtaaagatat cggcttccgg gttcaaagtt cagttctttt tgccggctta tcaatttcag
     3901 aatttgctgc gttgtcgtca cgtcgtccat tcgccatccg gctaaagtca gcaaagcagc
     3961 ctggtcttta atgccgctgg tgtgcgcgag caggtgtttt attttgataa tttttccggc
     4021 gtccggcagc tccgggacat attttctgac gtcgtcttcg agcgaaatct ttccttgttt
     4081 ttcgagaagg taaatcgaga atgcagtgaa ttgctttgaa accgaagcga tttggaaaac
     4141 cgtcttcgtc gtgaccggaa tatcatattc cagatttgcc gttccataac cttttttgaa
     4201 aattattttc ccgtctttga cgacggcaac agccactccg ggcgttgtcg aattgtaact
     4261 ggaaaaaagc cggtcgattt gtttatcaat cgcctcatca atattttgcc cgtacaaagc
     4321 agtgcagccg agtgctatga aaactatcga caagtaggat tttagtattc gattctcgga
     4381 tatatgcata atttgtttta aacattaagt ttctaccgct gtcataaccg gtctgcaaaa
     4441 tggcggctta cttttcttcg attcaaaaga aatatcaatc tccgtctgca aattatgtaa
     4501 gattgtaatg cggcaacgat tattccgaac cgacttttcg ataaacggca caaataaccg
     4561 ttaagcggtc aggtcgagca tcattcctac attcgaaagt gttcttttca gagaattctt
     4621 tgtttcggac tgtcacgcga aagttaaatc ggcgagcgcg aaaacagaag aaaacagcga
     4681 ggaaatagaa aaaggtgcag acaccggcgg agccgcgctg tcaaacacct ttaagttatt
     4741 gctcccgaaa cgatttgaac gttcgaccta ccgcttagga ggcggttgct ctatccagct
     4801 gagctacggg agcaaacgat tttagatttt agattttaga ttgccgattg tccagaagtt
     4861 gcggcaaaaa taaaattacg cgctttgttt cgcggcttgc attcggcatt aattaaacat
     4921 cgtatcgaag cagcgagctg acgttgtagc cgtcgaattt ggcgcgcccg ttcagaaaat
     4981 tcaattcgac gacgaacgaa agaccgacga ttttgccgcc caagccttcg accaaatcga
     5041 cgaccgcttt cgccgttccg ccggtcgcca gcaaatcatc gacaatcaaa acgcgatgtc
     5101 cttcgccgac ggcgtcgcga tgcatttcca gcgtgtcctg cccgtattcc aaatcatacg
     5161 agaccgaaac tttttcggcg ggcagtttcg tcggtttcgt gcccgtccgc aagccgaaga
     5221 aactgcccgc cgta
//
LOCUS       DASWNE010000721         3798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_445679, whole genome shotgun sequence.
ACCESSION   DASWNE010000721 DASWNE010000000
VERSION     DASWNE010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445679"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..2056)
                     /locus_tag="VGB68_14125"
     CDS             complement(74..2056)
                     /locus_tag="VGB68_14125"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HEY0050426.1"
                     /translation="MKQFTRLFTFFFFLLASINVAAATTAAGDEVPSWFAQFRTMQAP
                     TYEKDVKAVVLQKDSIVTVGSDGKAVTTMTYVVRILSREGRREAVAVEPYLVSSGKIR
                     EIKGWLIRADGTTKAYGKDQVLDRISDPDDIYNELRLKIIDASDEADAGAIFGYQVTS
                     EEMPLFDQDSWLFQDNLPALVSRYTLNLPNGWKAESNTFNHTKIQPSVNGSTYVWELR
                     NLQPIAEEPESPSIQNLVPRLAVSYSPSATTTNFRTFKDWTEVSRWNTEISDPSVIID
                     DNIANKARELTANAKTEFEKIRAIGSFVQNLQYISIDIGVGRGNGYRPRPSTLVLQRG
                     YGDCKDKANLMRALLKVLKIEAYPVAIYSGDPNFVREEWASPRQFNHCIIAVKVGDET
                     IAPTIIAHPTLGRLMIFDATDPYTPVGDLPDYLQGSFALIVAGNSGGLMKMPVTAPEA
                     NKMMRSVEMTIADNGDISGVIRERAQGQQAASARRLQRAISVSDYNKMIEGWVVRGIS
                     TARVGKISPVDKNSGENQFDLDVELKAPVYGQLMQNRLLVFKPVVVDRLNSLRLTDNK
                     RTHPVIIEANAFSETAVFTLPSGFVVDETPDAVNLETPFGKYSTSYEIKDNSLIFKRS
                     LTLNSTTIPVDKYASVKDFFVKIRSAEQSPVVLMRK"
     gene            complement(2106..>3798)
                     /locus_tag="VGB68_14130"
     CDS             complement(2106..>3798)
                     /locus_tag="VGB68_14130"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEY0050427.1"
                     /translation="ARVIKADGSIVELQEKDIFEREIIKTDGLKVKAKSFAVPNIEPG
                     VIIEYRYREVIDDASARGMRLNFQRDVPVQNLSFYYKPYNDKTPNYQTFNTDVRFNKD
                     KNGYWLASQSNVVSKKEEPRMPPEDEVTPWLLIQGVSLNLINASMTSISFTIKDPSSP
                     QRYWGAVSAEKAELVKVMNKQNGDIKKTAEAVTASASTPEEKLQKLYEFAQTQVKNVS
                     FDAALTDEMRAKLPTNKSIADVLKRKSGNSGEVDMLFGAMATALGFDARIAFSGNRSE
                     KFFDPEMTNDFFIHPAAIAVNVDGNWKFFNPGVPFLPYGTLVWYEQGVWAMLVSEKGY
                     AWLKTPLADYSDSRARRTGKFKLAEDGTLEGSVRIEYSGQPGILRKLGGYDDSADKRV
                     ENLKEEMKRQMSTAEISDVTIENITDPVKPVVYAFKVRVPNYAQKTGKRLFFQPGFFE
                     YGATPVFSSASRKYDIYFQYPWSEQDSVEIEYPKTFDLDNADSPGAIADPKRITMLDI
                     SIKADRDNAVLFYNRKFHFGGGGNILFSAAQYQPLKGMFDAFHKADSHTITLRQK"
BASE COUNT          790 a         1030 c         1010 g          968 t
ORIGIN      
        1 atgaaaattg cattttcgaa tttccttcaa caaacacctg accgctgacg gctgatggct
       61 ggctacttta gttttatttt cgcatcagca cgacgggcga ctgttcggcg gagcggattt
      121 tcacgaagaa atccttgacc gaagcgtatt tgtcgaccgg aatcgtcgtc gagttcagag
      181 tgagcgagcg tttaaaaatc aggctgttgt ccttgatttc ataactcgtg gaatatttgc
      241 cgaaaggcgt ttccagatta accgcgtcgg gcgtttcgtc tacgacaaaa ccgctcggca
      301 gcgtgaaaac ggcggtttcg ctgaaagcat ttgcctctat aatcaccgga tgagttcgtt
      361 tattgtcggt caggcggagc gagttcaggc ggtcgaccac gacgggttta aagaccagca
      421 ggcgattttg catcaattga ccgtaaaccg gcgctttcaa ctcgacatcg aggtcaaact
      481 gattttcgcc gctgttttta tcgaccggcg agattttgcc gacgcgcgcc gtcgagatgc
      541 cgcgcacgac ccagccctca atcatcttgt tgtaatcgct gaccgagatt gcccgctgca
      601 gtcttcgggc agatgccgcc tgctgtccct gcgcgcgctc gcgaatcacg ccgctgatgt
      661 cgccgttgtc ggcgatggtc atttccacgc tgcgcatcat tttattggct tccggcgcag
      721 tgaccggcat tttcatcagc ccgccgctgt ttccggcaac gattaaagca aagcttccct
      781 gcaaataatc gggcaaatcg ccgaccggcg tatacgggtc ggtcgcgtcg aaaatcatca
      841 ggcgtccgag cgtcggatgc gcgataatgg tcggcgcgat ggtttcgtcg ccgactttga
      901 cggcgataat acaatgattg aactggcgcg gcgaagccca ttcttcgcgg acgaaattcg
      961 ggtcgcccga ataaatcgcc accggataag cttcgatttt gaggactttg agcagcgcgc
     1021 gcatcagatt cgctttgtct ttgcaatcgc cgtaaccgcg ctgcaaaacc agcgtcgaag
     1081 gacgcgggcg ataaccgttg cctctgccga cgccgatgtc aatcgagatg tattgcagat
     1141 tctgcacgaa gctgccgatg gcgcgaattt tttcgaattc ggttttcgcg ttcgccgtca
     1201 gctcgcgcgc tttgttggcg atattgtcgt cgataatcac ggacgggtcg ctgatttccg
     1261 tattccagcg cgagacttcc gtccagtctt taaaagtgcg aaaattcgtc gtcgtcgccg
     1321 acggcgagta gctgacggcg agccgcggca ccagattctg aatgctcggg ctttccggtt
     1381 cttcggcaat cggctgtaaa ttgcgcagtt cccagacata ggtcgagccg ttgacgctcg
     1441 gctgaatctt tgtgtggtta aaagtattgc tttccgcttt ccagccgttc ggcagattca
     1501 gcgtgtagcg cgaaacaagc gccggcagat tgtcctgaaa aagccagctg tcctggtcga
     1561 aaagcggcat ttcctcgctc gtcacctgat aaccgaaaat cgcgcccgcg tccgcttcgt
     1621 ccgaagcgtc gatgattttc aggcgcagct cgttgtagat gtcgtccggg tcggagattc
     1681 tgtccagaac ctggtctttt ccgtaagctt tcgtcgtgcc gtcggcgcga atcagccagc
     1741 ctttgatttc acggattttg cccgagctga ccagataagg ctcgaccgcg acggcttcgc
     1801 ggcgtccttc gcgcgagagg atgcgcacaa cataagtcat cgtcgtcacg gctttgccgt
     1861 ccgagccgac cgtaacgatt gaatcttttt gcagcacgac ggctttcacg tctttctcgt
     1921 aggtcggcgc ctgcatagtg cgaaactgag caaaccacga aggcacttcg tcgccggcgg
     1981 cggtggtcgc cgccgcgaca tttatcgacg ctaaaagaaa gaaaaagaaa gtaaataaac
     2041 gagtaaactg tttcatatta actccggaaa tcagggcaga cgggtgaaga ctgccccgtc
     2101 aaaaactact tttgtctgag cgtaatcgtg tgcgaatccg ctttgtgaaa agcatcaaac
     2161 atacctttca gcggctgata ctgcgccgcc gaaaacaata tattgccgcc gccgccgaaa
     2221 tggaatttgc ggttgtaaaa caacacggcg ttgtcgcggt cggctttaat gcttatgtcg
     2281 agcatagtga ttctcttcgg gtcggcgatg gcgccgggcg agtcggcgtt gtccaaatcg
     2341 aaggttttcg gatattcgat ttcgacgctg tcctgttccg accacggata ttgaaaataa
     2401 atgtcgtatt tacgcgacgc gctcgaaaac acgggagttg ccccgtattc gaaaaatccg
     2461 ggttgaaaaa agagtctctt gccggttttc tgcgcgtaat tcggcacgcg aactttaaac
     2521 gcgtaaacga cgggcttgac cgggtcggta atattttcaa tcgtcacgtc ggaaatctcc
     2581 gccgtgctca tctgccgttt catttcctct ttcaggtttt cgacgcgctt gtctgccgaa
     2641 tcgtcgtagc cgccgagttt tcggagaatt ccgggctgtc ccgaatactc gatgcgcacg
     2701 ctgccttcca gcgttccgtc ttccgccagt ttaaatttac ccgtccgccg ggcgcggctg
     2761 tcgctataat cggcaagcgg cgttttcagc cacgcgtagc ctttctcgct gaccagcatc
     2821 gcccaaactc cctgttcata ccagaccagc gtcccgtagg gcagaaaagg cacgcccgga
     2881 ttaaagaatt tccagtttcc atcgacattg acggctatcg ccgccggatg aataaaaaag
     2941 tcgttggtca tttccgggtc gaaaaacttt tcgctgcgat ttcccgaaaa ggcgatgcgc
     3001 gcatcgaatc cgagcgcggt tgccatcgct ccgaacagca tatcgacttc gccgctgttg
     3061 ccggatttgc gttttaaaac atcggctatc gatttattcg tcggcagctt ggcgcgcatt
     3121 tcatcggtca gcgcggcgtc gaaagagacg tttttgactt gcgtctgggc aaattcgtaa
     3181 agcttttgca gcttctcttc cggcgtcgaa gccgaggcgg tcacggcttc cgccgttttt
     3241 ttaatatcgc cgttctgttt gttcatcacc ttgaccagct cggctttttc ggcgctgacc
     3301 gcgccccagt atcgctgcgg gctgctcggg tctttgatgg tgaaggaaat cgaggtcatg
     3361 ctggcgttga tgagattcag gctgacgccc tgaatcaaca gccacggcgt cacttcgtct
     3421 tcgggcggca ttctcggctc ttcttttttc gagacgacgt tgctctgcga agcgagccaa
     3481 tatccgtttt tatccttatt gaaccgcacg tccgtgttga aagtctggta attcggcgtt
     3541 ttgtcgttat aaggcttgta ataaaaggag aggttctgca ccggaacatc gcgctgaaaa
     3601 ttaagccgca tacctcttgc cgaagcgtcg tcgataactt cccgatagcg atattcgatt
     3661 atcacgcccg gctcgatatt cggcacggcg aaagatttcg ctttgacctt cagaccgtcg
     3721 gtcttgatta tctcgcgctc gaaaatatct ttttcctgaa gctcgacaat gctgccgtcc
     3781 gctttgatga cgcgcgcg
//
LOCUS       DASWNE010000722         4065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_201032, whole genome shotgun sequence.
ACCESSION   DASWNE010000722 DASWNE010000000
VERSION     DASWNE010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201032"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..1320
                     /gene="hisB"
                     /locus_tag="VGB68_14135"
                     /pseudo
     CDS             215..1320
                     /gene="hisB"
                     /locus_tag="VGB68_14135"
                     /inference="COORDINATES: protein motif:HMM:NF012687.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
     gene            1342..1935
                     /gene="hisH"
                     /locus_tag="VGB68_14140"
     CDS             1342..1935
                     /gene="hisH"
                     /locus_tag="VGB68_14140"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEY0050428.1"
                     /translation="MKTAIVKYNAGNVESVRNALRRLSVEPILTDDAEILSSADRVIF
                     PGVGEASGAMRFLRERNLDSVIKNLRQPVLGICLGMQLLCEFSEENSTECLGILPYRV
                     RRFESENLKIPQIGWNKIFALKTGLFRTVAENAYVYFVHGYYVEAGAETIGACDYGVK
                     FSAALNYKNFYAVQFHTEKSGAVGEKILENFLKICLK"
     gene            1923..2645
                     /gene="hisA"
                     /locus_tag="VGB68_14145"
     CDS             1923..2645
                     /gene="hisA"
                     /locus_tag="VGB68_14145"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEY0050429.1"
                     /translation="MFEIIPAIDLIEGRCVRLRQGDFAQKTIYNENPLEAAKEFENAG
                     LKRLHLVDLDGAKNGCLKNLKILESLARGTNLEIDFGGGIKTDEDINAVFDAGAKCAS
                     VGSTAVKNAEKFFAWLEKYGSEKILLGADVRGEKLAVDGWQTETEIEILPFLREYSAR
                     GVTQAFVTDISRDGLLAGSSNELYAEIRREISHLKLIASGGVSRIEDIFELKRIGCAG
                     VIVGKAIYEGRIELKDLAKYAG"
     gene            2635..3390
                     /gene="hisF"
                     /locus_tag="VGB68_14150"
     CDS             2635..3390
                     /gene="hisF"
                     /locus_tag="VGB68_14150"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEY0050430.1"
                     /translation="MLAKRIIPCLDIKNGRTVKGTNFLNLRDAGDAVELAKAYSQQGA
                     DELILLDITATDENRQTLRQLVKRVAAEINIPFTVGGGINTVKDIEVLLDSGADKVSI
                     NTAAVKNPRILFEAAKNFGSQCVVLAIDAKKVDENWKVFLNGGRVETELEAVEWARRG
                     ARLGAGEILLTSMNADGTKTGFEIDLTRAVSESLKIPVIASGGAGAPEHFIEVFENGK
                     ADAALAASVFHFREIEIPALKNYLRERGVDVRI"
     gene            3414..4007
                     /gene="hisIE"
                     /locus_tag="VGB68_14155"
     CDS             3414..4007
                     /gene="hisIE"
                     /locus_tag="VGB68_14155"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF002747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HEY0050431.1"
                     /translation="MKVDFEKYADRLVPAIVQDAETQKVLMLGFMNAESLRLTRKSGK
                     ATFFSRSRQKFWTKGETSGNFLEVVEITLDCDADTLLIKAKSGGTVCHTGADTCFDEK
                     NENEDFLFELEKIILERKANPAEDSYTTKLFRRGINKIAQKIGEEAVELVIEAKDDDR
                     ELFKNEAADLLFHLLVLLAAKEVSLDEVMQTLKKRKR"
BASE COUNT         1113 a          917 c         1112 g          923 t
ORIGIN      
        1 acaaattttt gctcggcgag cgaatcgtcg tgcgcaaccg cagccgcgtc gcgctttgcg
       61 cgggctgcct gcgaattaca atcggcacgc cggcggaaaa cgaaagcttg attgattcgc
      121 tcggaaaatt cgccgccgga aaggctgaaa gcggagagaa agcagcggcg aaatcataaa
      181 aattttgttc gactctaata atgttgaaaa tcctatgaaa aaagttttat ttattgaccg
      241 cgacgggaca atcgttgacg agccggaaga ttttcagata gacgcctttg aaaagctcag
      301 cttcctgccg ggcgtcatta ccaatctcgg caaaatcgcg cgcgagatgg actacgagct
      361 ggtgatgatt tccaatcagg acgggctcgg aaccgaaagc tttcccgaag caaattttta
      421 tccggtgcat aactttattt tgcggacgct ggaaaatgaa ggcgttctct tctcggaagt
      481 ttgcatcgac cgttcgcacg ccgccgaaaa caaaccgacg cgcaagccgg agaccggact
      541 tttgcaaaaa tacttttccg gcgaatacga ccttaaaaat tcatttgtta taggcgaccg
      601 cgaaaccgat atcgagctgg cgaaaaatct gggcgcgaag gcgatattta ttaaaaatcc
      661 gaactttaaa gcggctgcag cggaaaacgt tttcatcgcg gacgactggc gtttgattta
      721 cgagttttta aaacttcctc cgcgcaaaac ggcgcatcga cgctcgacca aagaaacgga
      781 aattaaagtc gagttgaatc tggacggaag cggaacggcg gagattgcaa ccggaatctc
      841 gtttttcgac catatgctcg aacagcttgc gcgacacggc gcaattgatt tgaaaatcga
      901 agtgaaaggc gatttacaca tgacgagcat cacacgattg aagacgtcgc cattacttta
      961 ggcgaaacgt ttgcgctcgc gctcgccgat aagcgcggaa tggagcgata cggtttttcg
     1021 ctgccgatgg acgattgcct cgcgcaggtc gcaattgatt tcggcgggcg caactggatt
     1081 gtctgggaag cggaatttcg ccgcgaaatg atcgggaaaa tgccgacgga aatgtttttt
     1141 catttcttca aatcatttac cgacaaagcg cagtgtaatt tgaacgtcaa agccgaaggc
     1201 gcgaacgagc atcacaagat tgaagcgatt tttaaagcct ttgccagggc gataaaaatg
     1261 gctgtcagcc gcgatgcccg caattgcggg ctgctgccct cgacgaaagg agttttgtga
     1321 gagaacaaag ctgctaatga tatgaaaaca gccatcgtca aatacaatgc gggaaacgtc
     1381 gaatcggtca gaaatgcctt gcgccggctg agcgtcgagc cgattttaac ggacgatgcg
     1441 gagattttaa gctcagccga ccgggtgatt tttccgggcg tcggcgaagc gtccggcgca
     1501 atgcgttttc tgcgcgagag aaatctcgac tcggtcatca aaaatctccg ccagccggtt
     1561 ttgggaatct gtctcggaat gcagcttttg tgcgagtttt ccgaggaaaa ctcgaccgaa
     1621 tgtctgggaa ttttgccgta ccgcgtgcgc agatttgaat ccgaaaatct gaaaataccg
     1681 caaatcggct ggaataaaat tttcgcttta aaaaccggtc tgtttcgcac agtggcggaa
     1741 aacgcctacg tttattttgt gcacggttac tacgtcgaag cgggcgcgga gacgatcggc
     1801 gcgtgcgatt acggagtgaa attttccgcc gctttaaatt ataaaaactt ttacgccgtc
     1861 cagtttcaca cggaaaaaag cggcgcggtc ggcgaaaaga ttctggagaa ttttttgaaa
     1921 atatgtttga aataattccc gccatcgatt taatcgaagg cagatgcgtg cgcctgcgac
     1981 aaggcgattt cgcgcagaag acgatttaca acgaaaatcc tctggaggca gccaaagaat
     2041 ttgaaaatgc cggtctgaaa cgcctgcacc tggtcgattt ggacggcgca aaaaacgggt
     2101 gtttaaaaaa ccttaaaatt ctggaaagcc tcgcccgcgg cacgaatctg gaaatcgact
     2161 tcggcggcgg aatcaaaacg gacgaagata taaacgcggt tttcgatgcc ggagcgaaat
     2221 gcgcgagcgt cggcagcacg gcggtgaaaa acgcggaaaa gtttttcgcc tggctggaaa
     2281 aatacgggag cgaaaagatt ctgctcggcg ccgacgttcg cggcgaaaaa ttagccgtcg
     2341 acggctggca gacggaaacg gaaatcgaaa tcctgccgtt tttgcgggaa tattccgcgc
     2401 gcggcgtcac gcaggctttc gtgaccgaca tttccagaga cgggctttta gcgggcagct
     2461 caaacgagct gtacgcggaa attcgccgcg aaatttcgca tttgaaattg attgcttcgg
     2521 gcggcgtttc gcgtatcgag gacatttttg aattgaaaag aatcggctgc gcgggcgtta
     2581 tcgtcggcaa ggcgatttac gaagggcgaa tcgaattgaa agatttggcg aaatatgctg
     2641 gctaaaagaa taattccgtg tctcgacata aaaaacgggc gcacggtaaa gggaacgaat
     2701 ttcttaaacc tgcgcgacgc gggcgatgcg gtcgagctgg cgaaagctta ttcgcagcaa
     2761 ggcgccgacg agctgattct gctcgatata acggcgaccg atgaaaaccg ccaaaccctg
     2821 cggcagctcg taaaaagagt cgccgcggaa ataaatattc cgtttacggt cggcggcgga
     2881 attaatacgg tcaaggatat tgaagttctg ctcgattcgg gcgcggacaa agtttcaatc
     2941 aatactgcgg cggtaaaaaa tcctcggatt ttatttgagg cggcaaagaa tttcggctcg
     3001 caatgcgtcg tgctggcgat tgacgcgaaa aaggttgacg aaaactggaa ggtttttcta
     3061 aacggcgggc gtgtcgagac cgaattggaa gccgtggaat gggcgcgacg cggcgctcgg
     3121 ctcggcgcgg gcgaaatcct gctgacttcg atgaacgccg acggcacgaa aaccggcttt
     3181 gagattgatt tgacgcgcgc cgtttccgag tcgctgaaaa ttccggttat cgcttcgggc
     3241 ggcgcgggcg cgcccgaaca tttcatagag gttttcgaaa acggcaaagc cgatgccgcg
     3301 ctcgccgcaa gcgtttttca ctttcgcgaa atcgagattc cggcgctgaa aaattatctg
     3361 cgcgagcgcg gcgtcgacgt gagaatttaa aagttgcggc tgttattgga aatatgaaag
     3421 ttgattttga aaaatacgcc gaccgtttag ttccggcgat tgtgcaggac gctgaaacgc
     3481 aaaaagtttt aatgctcggt tttatgaatg ccgaatcgct caggctcacg cggaaatcgg
     3541 gtaaagcgac atttttttcg cgctcgcggc aaaagttctg gacgaagggc gaaacgagcg
     3601 gaaattttct cgaagtcgta gaaattacgc tcgactgtga cgctgatacg cttctgataa
     3661 aagctaaatc gggcggaacg gtttgccaca ccggagccga cacttgtttt gacgaaaaaa
     3721 acgaaaatga agattttctg ttcgagcttg aaaaaatcat tctggaaaga aaggcaaatc
     3781 cggcggaaga ttcatatacg acaaaacttt ttcgtcgggg aataaacaaa atcgctcaaa
     3841 aaatcggcga agaagccgtc gagctggtta tcgaagcaaa agacgacgac cgcgagcttt
     3901 ttaaaaatga agccgccgac ttactctttc atttgctggt tttgctcgcc gcaaaagaag
     3961 tttcactgga cgaagttatg caaactttga aaaagagaaa aagataatct tttccgggcg
     4021 gttgttcgat ttcactcttc ttttgggaat tgttggaaca attcc
//
LOCUS       DASWNE010000723         3471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_589236, whole genome shotgun sequence.
ACCESSION   DASWNE010000723 DASWNE010000000
VERSION     DASWNE010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589236"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VGB68_14160"
     CDS             <1..209
                     /locus_tag="VGB68_14160"
                     /inference="COORDINATES: protein motif:HMM:NF027528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050432.1"
                     /translation="VAIILSGSGSSREHIQRLGRILRKHEGKPAVLYEVVTRDTTEER
                     ISQRRTDVRQFQDAKTRNGGGLFD"
     gene            complement(221..1414)
                     /locus_tag="VGB68_14165"
     CDS             complement(221..1414)
                     /locus_tag="VGB68_14165"
                     /inference="COORDINATES: protein motif:HMM:NF017442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF790 family protein"
                     /protein_id="HEY0050433.1"
                     /translation="MLTSDLAINWRRGNKIFPRLIRTADAGFLRDAENLIAVFEDFEG
                     KTRGELEAELEEYVGTGTDYRILRGFIKLLTDRCEFETASVAEPEEIRQKIFLAARAF
                     QPVLPDSERREEILRLVAEEFETDTETIFSNLYADLSGNQKLISFETIAPGDLLDRYN
                     LAQAQALLYKCVELKIRIAPLDSVNYRAIFGWIKHFGLIHSVVGNASNGYEITLTGAA
                     SLFHRSQKYGIRMAVFLPALLLCTGWKMSAEIAHKEGNVFYEISSEQTELNSCYFDEP
                     PYKNPDIEKLKKAWEKFAGKWRLEENREVVDLGKTAFIPDLVLISTGNEKIYLDVIGF
                     WTPKSLKKRLEEFHAADFRQFVFAASQELRGSREEPLWESENVLFYKTKIEPHLLVNM
                     AENLK"
     gene            complement(1473..2420)
                     /locus_tag="VGB68_14170"
     CDS             complement(1473..2420)
                     /locus_tag="VGB68_14170"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEY0050434.1"
                     /translation="MDITAIEQARELIKPYVRRTALEYNRTLSQKLGTNVFVKLELFQ
                     KTGAFKVRGAFNKLLSLSDEEKRRGVVAVSGGNHAQAVAYASSVLGVDAVILMPSTTP
                     RNYVDATLGYGAKVDLQPTIAEAFAEIKRFEAEGRIFVHPFDDPLVIAGQGTVGLEIL
                     EDLPEVTDIFVSIGGGGLAGGVATAVKASKPEVRIWGVETIGADAMSQALAAGHPVEL
                     PAITSIAKTLGAPAVSPATLALTERLLESVTVVSDAEAVEALQFILERLKVLTEPAAS
                     CTLAAASRLKNQFNRDSNVVLIFCGSNLSLDDLCRYASF"
     gene            2650..3102
                     /locus_tag="VGB68_14175"
     CDS             2650..3102
                     /locus_tag="VGB68_14175"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEY0050435.1"
                     /translation="MTVYTIGHGRHAWADFLNLLQKHEIEFLCDVRSAARSRWVQFNG
                     AVLSENLRENGIGYEHLPECGGKTIAKPEDLAQGIERILEISAEVKTAIMCSESQPLT
                     NHKTPKANCHRVGLLSPLLKAKGALQIIHILPNGEAIEFDENTFSSIW"
BASE COUNT          880 a          873 c          833 g          885 t
ORIGIN      
        1 cggtggcgat aattctttcc ggctcgggct cctcgcgcga gcacattcag cggctcggac
       61 gaattttgcg caaacacgaa ggcaaaccag ccgttttgta tgaagtcgtc acgcgcgaca
      121 cgaccgaaga gcgcatcagc cagcgccgaa ccgacgtccg tcaatttcag gacgcgaaaa
      181 ccagaaacgg cggcggcttg ttcgattaag gaaattaaaa tcatttaaga ttttccgcca
      241 tatttacaag cagatgcggt tcgattttcg ttttgtaaaa aaggacgttt tcgctttccc
      301 agagcggttc ttcgcggctt ccgcgcagtt cctgcgaggc ggcgaaaacg aattgccgga
      361 aatcggcggc gtgaaattct tccagccgtt ttttcagaga tttcggcgtc cagaagccga
      421 taacatcgag ataaattttt tcattcccgg tcgagattaa aaccaaatcc ggtataaaag
      481 ccgttttgcc caaatcgaca acttcgcggt tttcttccag acgccacttg cccgcgaatt
      541 tttcccaagc ctttttcagc ttttcgatgt ccggattttt gtacggcggc tcgtcgaaat
      601 agcaggaatt cagctcggtt tgctcgctgg aaatttcgta aaacacgttg ccttccttat
      661 gcgcgatttc ggcggacatt ttccagcccg tgcaaagcaa taaagcgggc agaaaaaccg
      721 ccatccgaat cccgtatttc tgcgagcgat gaaaaagcga agccgcgccc gtcagagtta
      781 tctcgtaacc gttcgaggcg tttccaacga cggaatgaat caagccgaaa tgttttatcc
      841 agccgaaaat cgcccgataa ttaaccgaat cgagcggcgc gatgcggatt ttcaattcga
      901 cgcatttata cagcaaagcc tgcgcctgcg ccagattgta acggtcgagc aaatctccgg
      961 gcgcgatggt ttcaaaagaa attaattttt gattgccgga caaatcggcg taaagatttg
     1021 aaaaaatcgt ttccgtatcg gtttcgaatt cttcggcgac aagccgtaaa atttcttcgc
     1081 gccgctcgga atccggcaaa acgggctgaa aggcgcgcgc cgcgagaaag attttttggc
     1141 gaatttcttc cggctcggcg acgctcgccg tttcaaactc acatctgtcg gtcagcagct
     1201 tgataaaccc gcgcaggatg cgataatccg ttcccgttcc gacgtattct tcgagctcgg
     1261 cttcgagctc gccgcgcgtc ttgccttcaa aatcctcgaa aacggcgatt aaattttcgg
     1321 cgtcgcgcaa aaatccggcg tcggcggtac gaatcagacg cggaaaaatt ttattgccgc
     1381 gccgccagtt gattgctaaa tctgaagtca acattatttt tcagccgctc gcgcccgaat
     1441 ttcctgtttt cgaaaattcg gatttaagat aactagaaac tggcgtaacg gcataaatca
     1501 tcgagcgaaa gattgcttcc gcagaagatt aacactacat tgctatcccg attaaattga
     1561 ttttttaaac gcgaagccgc cgccagcgtg caggaagccg ccggttcggt caaaactttc
     1621 aatcgttcga gaataaattg cagagcttcg accgcttcgg cgtcggaaac gaccgtcacg
     1681 ctttccagaa gccgctcggt caatgccaga gtcgcgggcg aaacggcggg cgcgccgagt
     1741 gttttggcaa tcgatgtaat cgccggaagt tcgaccggat gaccggcggc gagagcctgc
     1801 gacatcgcgt cggcgccgat tgtttccacg ccccagattc tgacttccgg cttggacgct
     1861 ttgacggctg tcgccacgcc gcccgccaaa ccgccgccgc cgatactgac gaagatgtcc
     1921 gttacttcgg gtaaatcttc taaaatctcc aagccgacag tgccttgtcc cgctatcact
     1981 aaagggtcgt caaacgggtg aacgaaaatt cgaccttccg cttcgaagcg tttaatttcg
     2041 gcgaaagcct cggcgatggt cggctgcaaa tcgactttcg cgccgtagcc gagcgttgcg
     2101 tcgacgtaat ttcgcggcgt tgtcgaaggc atcaggatga cggcgtcaac gcccaaaacg
     2161 ctcgaagcgt aagcgaccgc ttgcgcgtga ttgccgccgc tgacggcgac gacgccacgc
     2221 cttttttcct cgtcggataa gctcagcagc ttgttaaaag ctccgcggac tttaaatgcc
     2281 ccggtttttt gaaaaagttc gagcttgacg aaaacatttg tccccagttt ttggctcagc
     2341 gtccggttat attccagcgc agttcgcctg acataaggct taatcagctc gcgcgcctgt
     2401 tcgattgcag taatatccat aatttaatgt gcgtttgttg ccggaaacgc aaagcggctt
     2461 ctggtaaaaa cttttcgcag acgaaacgga tttccgaaaa gctcccggag gcaagcttct
     2521 tcactatttt taaaagtctg cgaaaaattc aattcaccag cttatcgcta acctttcgct
     2581 tcggcgaatc tggactaaat tcatttgtaa tcttactata tccatattaa taaatcggaa
     2641 agaaatttaa tgacagttta caccatcggt cacgggcgtc atgcctgggc ggattttcta
     2701 aatctgctgc aaaaacacga aatcgaattt ttgtgcgatg tcaggagcgc ggcgcgctcg
     2761 cgctgggtgc aatttaacgg ggcggttttg tcggaaaatt tgcgcgagaa cgggatcggc
     2821 tatgaacatc tgcccgaatg cggcggcaaa acgattgcca aacccgaaga tttggcgcag
     2881 ggaatcgaac gtattctgga aatttccgcc gaagtcaaaa ccgctattat gtgttcggaa
     2941 tcacagcctt taaccaatca caaaacgccg aaagcaaact gtcatcgcgt cggtttatta
     3001 tcgccgcttt tgaaagctaa aggcgcgttg caaataattc atattttgcc gaacggcgaa
     3061 gcgattgaat tcgacgaaaa tacattttcg tcaatctggt aaagcagcgc cgcaaattag
     3121 agattagccg caagacggca taaaaagaag aataaagaaa atcagccgtg aataatacga
     3181 atgagaggaa ttattattta ttgtttttat tcgcctaatt cgtgctattt gtaactaaat
     3241 cttttttgcg tcctttcgcg gcaaaatttc tttgcctctt ttgtgatttc ttaagaataa
     3301 aacagcagaa ggcgaaagaa accgtaaaag atttctttcg ccttctgctt tgattgatgt
     3361 aagctatcgc agtaaaattt actgctttaa atcctgcgct tgtgttttga cttcatcggt
     3421 aattgttttc tgcgcttcat ttgcaacctg tttcacgcgg tcaacgaggt c
//
LOCUS       DASWNE010000724         1789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_516935, whole genome shotgun sequence.
ACCESSION   DASWNE010000724 DASWNE010000000
VERSION     DASWNE010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516935"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1789
                     /locus_tag="VGB68_14180"
     CDS             <1..>1789
                     /locus_tag="VGB68_14180"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY0050436.1"
                     /translation="GWDEWQFLIPSGEVVGELQITSPVAGQTFRGGQEVPFKWTITTP
                     FPNTSFNAFLILDGDRKIISLGGGTNTGTALTPKMPLVSTDSARFAVAAYGTGNRQKW
                     FFSEPFAIRPDSRFVDAAPQITMTSPAPNQPIPAGGAVPIAWSAADDEALRRFDVQAS
                     TDGGRMWINIAENLPPTATSFNWQLPPGGSSVNDVRVRVLAVDRRFQNSSDGANRIFQ
                     IVAPQNAPPSVQVTFPSNNASFQVGKSTFVAAAAADSDGTIQRVEFYAAQNFVGQPSR
                     TLIGSDAAAPYQIGWDLLFSGDYVITAEAIDNRGAVAASAPITVRVPQSGAPLPIGAP
                     ELTSPVQGQVFAPGSNITLSAFAGTANAGAVVEFYNGTQLIGSDASEPYSIVWNNVPA
                     GRYTISAKAVAANGAEAISEHIDIIVGSPQAIRRNAYDFDGDGKSDISVFRPTDGVWY
                     LNRSSQGFAAVQFGISNDKLAPADFDGDGKTDIAVYRDNTWYLLRSQAGFTAVQFGAA
                     GDVPQPADYDGDGRAELAVFRPSNGSWYVSSLATSQFNGVQFGASGDKPVAADYDGDG
                     KADYAVYRPSDGTWYLLRSQQGFTGVQFGI"
BASE COUNT          366 a          505 c          532 g          386 t
ORIGIN      
        1 aggctgggac gaatggcagt ttttaatacc gtcgggcgag gtcgtcggcg agctgcaaat
       61 cacttcgccg gtcgccggac agacttttcg cggcggtcag gaagttccgt ttaaatggac
      121 gataacgacg ccgtttccaa atacgtcttt caatgccttt ttgattctcg acggcgaccg
      181 taaaattatt tcgctgggcg gcggaacgaa taccggaacc gctctgactc cgaaaatgcc
      241 gctcgtcagc accgattcgg cgagatttgc cgtcgccgct tacggcacgg gaaatcgcca
      301 gaagtggttt ttcagcgagc ctttcgccat tcgccccgac tcgcgttttg tcgacgccgc
      361 gccgcaaatc actatgactt cgcccgcgcc gaatcaaccg ataccggctg gcggcgcggt
      421 tccgattgcg tggtcggcag ccgacgacga agcgctgcgc cgttttgacg tgcaggcttc
      481 gaccgacggc ggcagaatgt ggattaacat agccgaaaat ttgccgccga cggcgacaag
      541 cttcaactgg caattgccgc cgggcggcag ttcggttaat gatgtccgag tgcgcgtctt
      601 ggcggttgac cggcgttttc aaaattcatc cgacggcgca aatcgcatat ttcaaattgt
      661 cgccccgcaa aacgcgccgc cgagcgtgca agtcactttt ccgtcgaata acgcgagttt
      721 ccaggtcggc aaaagcactt ttgtcgccgc cgccgccgcg gacagcgacg gcacgattca
      781 gcgggtcgag ttttacgccg cgcaaaattt tgtcgggcag ccgagccgga ctttaattgg
      841 ttcggacgcc gccgcgccgt atcaaatcgg ctgggatttg cttttctcgg gcgattacgt
      901 gataacggct gaggcgattg ataatcgcgg cgcagttgcc gcttcggctc ccattaccgt
      961 cagagtgcca cagtccggcg cgccgctgcc gataggcgcg ccggaattga ccagccctgt
     1021 acaaggtcag gttttcgcgc ccggctctaa tattactttg agcgcatttg ccggaactgc
     1081 caacgccgga gcggtcgtgg agttttataa cggcactcag cttatcgggt cggatgcgag
     1141 cgagccttac agcatcgtct ggaacaatgt tccggctggg cgctacacga tttccgccaa
     1201 agccgtcgcc gccaacggcg ccgaagcgat ttcagaacac atagatatta tcgtcggcag
     1261 cccgcaggcg atacggcgca atgcgtatga tttcgacggt gacgggaaat cagacatttc
     1321 ggttttccga ccgacagacg gcgtctggta tctgaatcgt tcgagtcagg gattcgccgc
     1381 cgttcaattc ggaatttcta acgataaact cgcaccggct gatttcgacg gcgacggcaa
     1441 aacagatatt gcggtttacc gcgacaacac gtggtatctg cttcggtcgc aagccggttt
     1501 tacagccgtg caattcggcg ctgccggaga cgtgccgcaa ccggctgatt acgacggcga
     1561 cgggcgggcg gaattagccg ttttcagacc gtcaaacggt agttggtatg tgtcgagctt
     1621 agcgaccagc cagtttaacg gcgtccagtt cggcgcaagc ggcgataagc cggtggcggc
     1681 ggattacgac ggcgacggca aagccgatta cgccgtttat cgaccgtcgg acggaacgtg
     1741 gtatctgctc agaagccagc agggatttac aggcgtgcag ttcggcatt
//
LOCUS       DASWNE010000725         3891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_60114, whole genome shotgun sequence.
ACCESSION   DASWNE010000725 DASWNE010000000
VERSION     DASWNE010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60114"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1607)
                     /locus_tag="VGB68_14185"
     CDS             complement(<1..1607)
                     /locus_tag="VGB68_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050437.1"
                     /translation="MRAINFIAKNNQYQVEELNKNTRVSREKQAGKIVGSLNYKKITA
                     SIFSSLLYLCLSLFLSSTVFGQSQSTTNAADQTLRSNGRVNPSSLGMEMDIPLGSYPA
                     RGINLPLGLSYSSKLWRFEPYTTVDVGSHSNVYVIPKYSENAASGWTTSLSQPYIEYT
                     GEMNLFDDVGRPLQEVYSQGQQTPAGENYVRRITVYLPGGGSHELVAEMEPIATSGYG
                     QGAPASAWEGTFYATDGSGVKYVQNSAANPAVYRLYMPDGSFYDFNPTRENSLKQPRR
                     AYRLTDAHGNYAQFYAATTAYPNGYWKDTLGRDFPVMIPGEAPAMPSNTEVLTQSFAM
                     PGIDPNTPYKLRWKRLQGSSAATSAFTPNQNYQLKYAGHTQTGASPTTYGPTLFYDNT
                     QISSCSGDKLKVLESGVLFNPVVLSEVELPNGAKYRFSYNEYGEIDRIDYPTGGYESI
                     HYDKVASLAELGAPYQMSNRGVTLREIYESVNDSTADEWTYTAQSSANNYRTSVIAPD
                     GTRTDRFMHRGVPGPGCVGPDAVGTSM"
     gene            complement(1720..2994)
                     /locus_tag="VGB68_14190"
     CDS             complement(1720..2994)
                     /locus_tag="VGB68_14190"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0050438.1"
                     /translation="MSKKKRSNQRKSNEPISLGLSGAHSGQSTGNTVIDSSNPSQSDE
                     SSLTNKTGVLSYIKNSSNWWVIAIVVFFAIGAMGAGLKYLDESAKKERAANRNASSSQ
                     QNQSLLSKLNPFANPTPTPTPLPLSKEYIYAGSRLLAVEDANANAAPPADLAVWRPSS
                     GYWYVMGVTQQQWGSGSSNDKPVPGDYDGDGKTDFAIYRPTTGAGIWCVIKSSTGADE
                     YYNFGTSGDVPKAADFDGDGKSDAALFRPSNGTWYIRYSTTGNLVTQQFGTNGDVPVP
                     ADFDGDGKADIAVFRPGTSGYFYIFRSSDGQLQSAQFGLSGDEPHPADYDGDGKADFA
                     VWRTSNGNWHINQSSNNTTVSYQWGVYTQDTAVPNDYDGDGKVDVAVWRGSSSNSNEI
                     GSWYIRNSSTGQMRTESWGQTGDTPVPALYRR"
BASE COUNT          910 a          938 c          958 g         1085 t
ORIGIN      
        1 ttcatgctcg ttcctaccgc gtccggacct acgcagcccg gacccggcac gcctcgatgc
       61 ataaagcggt cggtgcgcgt gccgtccggc gcgatgacgc tggtgcggta gttattggct
      121 gacgactgcg ccgtgtaagt ccattcatcg gcggtcgaat cattgacgct ttcgtatatt
      181 tcccgcaacg tgacgccgcg gttcgacatc tggtaggggg ctccgagttc cgccaacgac
      241 gcgaccttat cataatgaat cgactcataa ccgccggtcg gataatcaat ccggtcaatc
      301 tcgccgtact cgttgtaact aaaacggtat ttcgccccgt tcggcagctc gacttccgac
      361 agcacgactg gattaaataa cactcctgat tccagcactt ttaatttatc gcccgaacag
      421 gagctgatct gcgtattgtc gtaaaaaagt gtcggaccgt aagtggtcgg cgacgcgccc
      481 gtctgggtgt gaccggcgta tttcaactgg taattctggt tcggcgtgaa ggcactcgtc
      541 gcggcgctac ttccctggag gcgtttccag cgcaatttat aaggggtgtt cgggtcgatg
      601 cccggcatgg caaatgactg cgtcagcact tcggtattgc tcggcatcgc cggagcttcg
      661 cccgggatca tcaccgggaa atcgcgtccc agagtatcct tccagtaacc atttggataa
      721 gcggtcgtcg cagcgtaaaa ctgggcatag ttgccgtgcg cgtcggtcag gcgataagcg
      781 cggcgcggct gtttcaagct attctcgcgc gtcgggttga aatcatagaa agaaccgtcc
      841 ggcatataaa gacggtagac ggcgggattg gcggcgctgt tctggacgta ttttacgccc
      901 gagccatccg tcgcgtagaa ggttccttcc caggcgctcg ccggcgctcc ctgcccgtac
      961 cccgatgtcg caatcggctc catctcggcg acgagctcgt gagagccgcc gcccggcagg
     1021 taaacggtga tccgccggac gtagttttct cccgcaggag tttgttgtcc ctgcgagtaa
     1081 acttcctgga gcggtctgcc gacatcgtca aagaggttca tctcaccggt gtattcgata
     1141 tagggttgcg acaaactggt tgtccagccc gaggcggcgt tctccgaata tttaggtatg
     1201 acgtaaacgt tcgagtggct gcctacgtct acagtcgtgt acggctcaaa ccgccagagt
     1261 ttcgacgaat agctcaagcc gagcggcaga ttaatgccgc gcgccggata tgaaccgagc
     1321 gggatgtcca tctccatacc gagcgacgaa ggattcacgc gcccgttcga gcgcagcgtc
     1381 tggtcggcgg cgttcgtcgt cgattgcgat tgaccaaaaa ctgtcgagga taaaaataag
     1441 ctcagacaca agtataatag ggacgagaaa atactcgcgg taattttctt ataattcaaa
     1501 ctgccgacaa ttttgcctgc ttgtttttct cgacttactc tggtgttttt attcaattcc
     1561 tcgacctgat attgattatt tttcgcaatg aagtttatcg ctcgcatatt ctgatcctca
     1621 attttgcccg gcgttgcttt tcttttattc ctttcggttt cgccgccggt agtttcaaaa
     1681 atttttatga aaaagctctg acttttagtt ctcttaattc tatcgacgat ataacgccgg
     1741 gacaggagta tcacctgttt gtccccaact ttcggttctc atctgcccgg tcgagctgtt
     1801 tctgatatac cagctgccga tttcgttcga gtttgaagaa ctgcctcgcc aaacggcgac
     1861 gtcgacttta ccgtctccgt catagtcgtt cggcaccgcc gtgtcctgag tgtaaacgcc
     1921 ccattgataa ctgaccgtcg tgttgttcga gctttgattg atatgccagt tgccgtttga
     1981 cgtgcgccag acggcgaaat ccgctttgcc gtcgccgtcg taatcagccg gatgcggctc
     2041 gtcgccgctt aatccgaact gagcggattg aagctgaccg tcggagcttc tgaaaatgta
     2101 gaaatatccg gaagtgcccg ggcgaaaaac tgcgatgtcg gcttttccgt cgccgtcgaa
     2161 atctgccggc acgggaacat cgccgtttgt gccgaactgc tgcgtgacca agtttccggt
     2221 cgtgctgtaa cggatatacc aggtgccgtt tgacgggcga aacagagcag catcggattt
     2281 accgtcgccg tcgaagtcag ccgccttcgg aacatcgccg cttgttccga agttgtagta
     2341 ttcgtccgcg cccgtgctgc ttttgatgac gcaccagatg cccgctccgg tggtcgggcg
     2401 gtagatcgca aaatcggtct tgccgtcacc gtcgtaatcg ccgggcacgg gcttatcgtt
     2461 gctggagccg ctgccccatt gctgctgcgt gactcccatt acgtaccagt aaccggatga
     2521 tggtcgccag acagctaaat ctgcgggcgg cgcggcatta gcattagcat cttcgactgc
     2581 cagcaatctg ctgccggcat agatgtattc ttttgataac ggtaatggcg tcggcgttgg
     2641 agtcggattg gcgaagggat tgagttttga taataagctt tgattttgtt gactggaaga
     2701 agcgtttcga ttggctgctc tttctttctt tgcggattcg tccagatatt ttagccccgc
     2761 gcccatcgct ccaattgcga agaatacaac aatggcgatg acccaccagt ttgacgagtt
     2821 tttgatatat gagaggactc ccgttttatt tgtcagactt gattcgtcag actggcttgg
     2881 attggatgaa tcgataacgg tattacccgt cgattgaccg gaatgagctc ccgacaagcc
     2941 taaactgata ggctcgtttg attttcgctg attgcttctt ttctttttgg acataatgtt
     3001 tcctgaaatc aaagactttt acttttgttt gctgagcgtt gagtgagcgc aaattctgcc
     3061 gctgaaagac aagctttcgc cgttaaatgg cacaacaaca agctcaatgt tttttatttt
     3121 gtagctgcct gaaaaggaaa aagcggtaga tgcagaatat caagtaaaga tatatctctt
     3181 gaggtattta tcgatttttg ttgataagac gcaaaaatcg aatgtttaat ttaccgatta
     3241 atcactgaga ttttcttttt cagaactgat ttttaagtgt tcccaactta ctgaacttaa
     3301 accctgctgg atgaaaataa ctgctaatca aaagcaatta ttaattgcta aagtgtaaac
     3361 tttctaattt tgagttgaaa taagtgttaa tagatgcgcg cttttaagaa aagttgcttg
     3421 aagttttaga gtgcaaaatt ttttgttaaa cagaaattca agaaattttt gtcgaatctt
     3481 agtatggagt cctgagaatt ctgatttgag acataaaaaa agacatagtt ttcactaggt
     3541 ttaaaaacta cactttctga gacattacgc tctaatactt ttcaaataat aatcgatgcg
     3601 caaaaagcct tgaaattgtg aaattttaaa cctagatttt ataggcatcc ggcaaattga
     3661 atttcttctg agcaaaatcc ttagtttttg cccaccctgt tttatactgc gagaacaaat
     3721 atttaatcag gcttccaaac atctatagcg atatacttct gtgctaaact cgttcgtaaa
     3781 aactaagatt ttaggcataa gaaaccatgt atttaaaaag gtcattcgca catattttga
     3841 atccattgcg attaagctct gaagaaattg aagaacttcg tgtgaatcgt g
//
LOCUS       DASWNE010000726         2255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_201139, whole genome shotgun sequence.
ACCESSION   DASWNE010000726 DASWNE010000000
VERSION     DASWNE010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201139"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1720)
                     /gene="nrfD"
                     /locus_tag="VGB68_14195"
     CDS             complement(143..1720)
                     /gene="nrfD"
                     /locus_tag="VGB68_14195"
                     /inference="COORDINATES: protein motif:HMM:NF025940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEY0050439.1"
                     /translation="MNYGFIIDNRKCIGCHACTVACKSEHEVPVGVNRTWVKYIEKGT
                     FPDTRRLFSVMRCNHCADAPCVEICPVTALFTRSDGIVDFDTRRCIGCKACTQACPYD
                     ALYMHPDEHTAAKCNYCSHRTDIGLEPACVNVCPEHAIISGDMDNPSSEISQLLAREV
                     VQVRKPEKGTKPKLYYIDGDTSSLKPTEANPDTDYMWSSQSSGVGHYAKFAEERIKTN
                     ADKSVPQKLVQIEPRGKSKSADKPRRVYDAPGKGVLWGWEVSAYILTKAIAAGAFLVP
                     VVGSYFMRVPETHTRIGAVIAFIFLAITGVLLIMDLGRPDRFLNVLFRPQWKSWLSRG
                     AYIITAYGGILTLWLLAAFLGFRDFLQYIQLFGIVFALLSAIYTAFLFAQAKGRDFWQ
                     SPMLGLHMLVHALMAGFAVFLLTGLFVQIDDRFLTSVTFLTIIALIFHLVTLAIELTT
                     THTTDDAHATVKMITNGEFSRAFWFGMILVGNILPLILLLAGNIRAFALAGVLILTGL
                     YFAQHIWVKAPQRIPLS"
BASE COUNT          595 a          540 c          560 g          560 t
ORIGIN      
        1 cttcacgtag agaatttgta attaaatcaa gaatatagtc aggtgatttt atgattgctc
       61 cgtttggcgc aagaattctt tcaatacgag caataagctc ttcaaaatct cttccggcac
      121 gtgacttctt tctcattttt atttaactca aaggaattct ctgcggcgct ttgacccaga
      181 tatgctgggc gaaatacaag cccgttaaaa ttaaaactcc ggctagagcg aaagcccgga
      241 tgtttccggc taacagcagt atcagaggta aaatgttgcc gaccagaatc attccgaacc
      301 agaaggcgcg gctgaattcg ccgtttgtaa tcatttttac ggtggcgtgc gcgtcgtcgg
      361 tcgtgtgcgt ggtcgtcagt tcgatggcta aagttaccag gtgaaaaatc aatgcgatga
      421 ttgttaaaaa cgtcaccgac gtcagaaaac ggtcgtcgat ttgaacaaat aaaccggtga
      481 gcagaaaaac ggcaaaacct gccatcagcg cgtgaaccag catatgcagt ccgagcatcg
      541 ggctttgcca aaaatctcgt cctttcgcct gcgcgaaaag aaatgcggtg taaatcgccg
      601 aaagcagtgc gaaaacgatg ccgaacagct ggatgtattg cagaaaatct ctaaaaccca
      661 gaaacgcggc gagcagccag agcgtcagaa ttccgccgta agccgtaatg atatacgcgc
      721 cgcgcgagag ccaggatttc cattgcgggc ggaaaagaac gtttagaaat ctgtccggtc
      781 gccccaaatc cataatcagc aaaacgcccg taatcgccag gaaaataaag gcgatgaccg
      841 cgccgattcg cgtgtgcgtc tccggaaccc tcataaaata gctgcctaca accgggacta
      901 aaaacgctcc ggcggcgatg gctttggtca aaatataagc ggagacttcc cagccccaga
      961 gcacgccttt gccgggcgcg tcgtaaactc ggcgcggctt gtcggctgat ttggatttgc
     1021 ctcgcggctc gatttgaacc aatttctgtg gcacggattt gtcggcgttg gttttgattc
     1081 gctcttcggc aaatttggcg taatgcccga cgccggacga ttggctcgac cacatataat
     1141 cggtgtcggg attggcttcg gtcggtttta aagaagaagt gtcgccgtcg atgtaataaa
     1201 gtttcggctt cgtgcctttt tcgggtttgc gaacctgtac gacttcgcgc gccagaagtt
     1261 gagaaatctc gctcgaagga ttgtccatat cgccggaaat aatggcgtgt tcgggacaga
     1321 cattgacgca ggcgggttcc aagccgatat cggtgcggtg cgagcagtag ttgcatttag
     1381 cggcggtgtg ctcgtccgga tgcatataaa gcgcgtcgta cgggcaggct tgcgtgcagg
     1441 ctttgcagcc gatgcagcgg cgcgtgtcaa aatcgacgat gccgtcgctg cgcgtaaaaa
     1501 gcgccgtcac cggacagatt tcgacgcacg gcgcgtcggc gcagtggttg cagcgcatca
     1561 ccgaaaaaag gcggcgcgta tctggaaaag ttcccttttc gatatacttt acccaggttc
     1621 gattaacgcc gaccggaact tcgtgctccg atttgcacgc taccgtgcag gcatgacaac
     1681 cgatacactt gcggttatca atgataaaac cataattcat tataaagccc tcattccagt
     1741 agcgttgttt ctgcacattt ttaagcggca aatgtgaaga aataagcgaa actcagtaaa
     1801 actataataa actaaaatgt gaagattgct gaataacccg aagtattccg aggctggaaa
     1861 aactaaaata aaatgtcctg taacactttt gcgcgtttag tctaaaactc ggattcttcc
     1921 cggctcaaca cttattgcag caaaaaatgg cgcgtaattc aaacaaaaac ggcgatttta
     1981 tttcgctttt cggttgctgc ctttttgtcc gctgaaaaag tgaaaaggcg aaaaagtgaa
     2041 aaagcgcctg acggttttcg acaagcggct tttttcactg tttgaccttt taatttttct
     2101 ccttttcccc ttttaacttt taaaccagct ttccataacc gaatcctgaa ggattatcgt
     2161 ctggtcgcgt tcgggtccgg tcgagattaa accgatttcg acgccgattg agctcgataa
     2221 aaattcgacg tattcacgcg ccttttccgg caaat
//
LOCUS       DASWNE010000727         2698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_94151, whole genome shotgun sequence.
ACCESSION   DASWNE010000727 DASWNE010000000
VERSION     DASWNE010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94151"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VGB68_14200"
     CDS             complement(<1..335)
                     /locus_tag="VGB68_14200"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY0050440.1"
                     /translation="MKILISGASGLVGTHLIPTLKAKNHEIFRLVRKATEAADEIRWD
                     AEKGFSDGERARLENFDAVVHLAGDNVASESWSEEKKRKIRDSRVVGTRVLVDALKSL
                     QNPPPVLVSA"
     gene            complement(345..698)
                     /locus_tag="VGB68_14205"
     CDS             complement(345..698)
                     /locus_tag="VGB68_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050441.1"
                     /translation="MSEEYPYQKKWERYRLYVRLWMIMFPLLFVPFVLLENDISFIPQ
                     KTLAYSIPLLWIVFIIVSLGVTSWKCPRCRRFYFALRSRINIASDFRCRNCGLEKYEG
                     SDIKSFNKKFGKRFG"
     gene            complement(789..1274)
                     /locus_tag="VGB68_14210"
     CDS             complement(789..1274)
                     /locus_tag="VGB68_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050442.1"
                     /translation="MKLLSIFLISLSLLFFPPQKDKIRLEIGAKLPAKLVAENKSIKL
                     IFTHTSQTRPYLEKEIKGVDYTIAYDGETRKIKYINTRDEDFVTGNKLRVGSEIEVKK
                     ENFYIIPQLEIRAPATPDGWQPVVGSDSDYIDFKSNFVDKLKDGETTKVTITGFSKGE
                     N"
     gene            complement(1359..1718)
                     /locus_tag="VGB68_14215"
     CDS             complement(1359..1718)
                     /locus_tag="VGB68_14215"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY0050443.1"
                     /translation="MLKLKVSLIFLFIVFLVVACVQTKSKRSGVVIAESKSYEASLYR
                     QNCAVCHGAEAYGKEIDGKFTPSLRFGEIEKKSEREIYEQIAHGKLPMPSFKNQLTEE
                     EIQKMVKFIRRDLQGKE"
     gene            complement(1748..2518)
                     /locus_tag="VGB68_14220"
     CDS             complement(1748..2518)
                     /locus_tag="VGB68_14220"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF017501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEY0050444.1"
                     /translation="MSDSFVLAGKTFNSRLIIGTGKYRSHREMQEAHAASGAEMVTVA
                     VNRVPLDRATESFLDYLSPQMRILPNTAGCYDAEHAIRTARLAREALETDWIKLEVIG
                     DKVTLLPDNEQTLEAARVLVKEGFVVLPYFSDDLIMAKKLLDIGCPAVMPLAAPIGSG
                     MGVQNPSNLRIMREQLPDAMIIVDAGVGVPSDAAIAMELGADAILMNTAIAEAGDAAQ
                     MATAMKLAVQAGRLAYLSGRMPKRLYASASSPIEGAIK"
BASE COUNT          658 a          685 c          628 g          727 t
ORIGIN      
        1 gcggaaacca gaaccggcgg cggattttgc agcgatttca aggcatcgac cagaacgcgt
       61 gtgccgacga cgcggctgtc gcggattttt cgtttctttt cttccgacca gctttcggag
      121 gcgacgttat cgcccgccag atgcacgacg gcgtcgaaat tttcgagccg cgcgcgctcg
      181 ccgtccgaaa aacctttttc ggcgtcccat cgaatctcgt ccgccgcttc cgttgctttc
      241 cgcacgagcc ggaaaatttc gtgatttttc gccttgaggg tcggaatcag atgcgttccg
      301 accagaccgc tcgcgcctga gattaaaatt ttcatagata aatattagcc gaacctttta
      361 ccgaattttt tgttaaagct tttaatatcc gagccctcgt atttttccaa gccgcaattc
      421 cggcaccgga aatcggacgc gatatttatt ctcgaccgca gagcaaaata aaaccggcgg
      481 cagcgcgggc atttccagga cgttacgccg agactaacga taataaaaac aatccagagc
      541 aaaggaatgc tgtaagccag cgttttttgc ggaataaaag aaatatcgtt ttccagcaat
      601 acgaacggca cgaacagcaa aggaaacatt atcatccaga gccgcacgta aagcctgtat
      661 ctctcccact ttttctgata cggatattct tcgctcaccg gttttttaat ttatccgaaa
      721 gcccgcgccc gcacggctcg cgcctgagac tggaattttc ataaataaac ccgattcggt
      781 tttaacttct aattttcgcc ttttgaaaag ccggtaattg tgactttcgt cgtttcgccg
      841 tcttttaatt tatcgacaaa attggatttg aaatcaatgt agtcggaatc gcttccgacg
      901 accggctgcc agccgtcggg cgtggcgggc gcgcgaattt cgagttgcgg aattatataa
      961 aaattctctt ttttaacctc gatttcgctg ccgacgcgca atttattgcc cgtcacgaaa
     1021 tcttcgtccc gcgtgtttat atactttatt ttgcgcgtct cgccgtcgta agcaatcgtg
     1081 taatcaacgc cttttatttc tttctccaga tacggtcgcg tttgactggt gtgcgtgaaa
     1141 atcaatttta tacttttgtt ttcggcaacc agtttcgcag gcagtttcgc gccgatttcg
     1201 agccggattt tatctttttg cggcggaaaa aatagcagac tcaagctgat aagaaatatt
     1261 gaaagtaatt tcataatcgt cacctttata agaaattcgt ttacaaccaa agaatagatg
     1321 ccggcaactg tcccgaaggt ttttacgcgc gagaaaaatt attcttttcc ctgtaaatcg
     1381 cgccggataa attttaccat tttctgaatt tcttcttcgg tcagctgatt tttaaacgaa
     1441 ggcatcggca gtttcccgtg agcgatttgc tcgtagattt cccgttcgct ctttttttcg
     1501 atttcgccga agcgcaggga aggcgtgaat tttccgtcga tttccttgcc gtaggcttcc
     1561 gcgccgtgac aaaccgcgca gttttgccgg taaagcgagg cttcgtaaga tttgctttcg
     1621 gcgataacga cgccgcttcg tttcgatttg gtttgaacgc aggcaacaac caaaaaaacg
     1681 ataaaaagaa aaattagcga aacttttaat ttcaacacaa ttttattttt acctttttcc
     1741 ttttgcctta ctttatcgcg ccttcaatcg ggctggacgc ggaagcgtaa agacgtttcg
     1801 gcattctgcc cgaaagatac gccagcctgc ccgcctgcac cgccagtttc atcgcggtcg
     1861 ccatctgcgc cgcgtctccg gcttcggcaa tcgccgtgtt catcagaatg gcgtccgcgc
     1921 cgagctccat cgcaatcgcc gcgtccgaag gaacgccgac gccggcatcg acgataatca
     1981 tcgcgtcggg cagctgctcg cgcataatcc gcagatttga aggattctgc acgcccattc
     2041 ccgaaccgat cggcgcggcg agcggcatca cggcgggaca gccgatgtcc aataattttt
     2101 tcgccataat caaatcgtcg gaaaaatacg gcaaaacgac gaagccttct ttgacgagaa
     2161 cgcgcgccgc ttccagagtc tgctcgttgt cgggcaaaag cgtgacttta tcgccgatga
     2221 cttcgagctt tatccaatcg gtttcgagcg cttcgcgagc cagccgagca gtgcgaatcg
     2281 cgtgttcggc gtcgtaacag ccagccgtgt tcggcaaaat ccgcatctgc ggcgagagat
     2341 aatcgagaaa cgattcggtg gcgcggtcga gcggcacgcg gttgacggcg accgtcacca
     2401 tctccgcgcc cgaagccgcg tgcgcttcct gcatttcgcg gtgcgagcga tattttcccg
     2461 tgccgatgat gaggcgcgag ttaaaagttt ttccggctaa aacgaatgaa tcggacataa
     2521 aatcaattac gaattaaaaa ttacgaatta cgagttttaa gaaatagatt gtaaatttac
     2581 agtttcaatc ttaacaaaaa atcaggcaaa agaaatgcgg actaaaatta gggtagtgtc
     2641 ttaaatctgt ggtgctattc ttaaaatatc ttttagcgtc caaaatgtct ttgtgatg
//
LOCUS       DASWNE010000728         2204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_517011, whole genome shotgun sequence.
ACCESSION   DASWNE010000728 DASWNE010000000
VERSION     DASWNE010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517011"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VGB68_14225"
     CDS             <1..517
                     /locus_tag="VGB68_14225"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEY0050445.1"
                     /translation="FVALNDEEDANWIQFIQNSVGAILSPFDSFLVLRGTKTLAVRME
                     KHDENGRAVANFLAEHPKVRKVYYPGLASHPQHEIAKRQQKGFGGMVSFETGSLEMAK
                     KVLESVKLCTLAESLGGVESLISHPATMTHASVPQEKRDQLGITDGLVRVSVGIEDIE
                     DIIEDLDQALS"
     gene            670..1533
                     /locus_tag="VGB68_14230"
     CDS             670..1533
                     /locus_tag="VGB68_14230"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEY0050446.1"
                     /translation="MLQIADSQTWTSSQDPSDFIIESQQFEVDASGVILAVSDGMGGA
                     LAGEVASKMAVETISEKILNEDPESTVSPHSTGDSLIAKLYEATIYANHLIHREQRSN
                     PEYSGMGATFTGIGVTREAVDIVQVGDSRAYLIRNAKIYQITKDQSLVQQLIDAQQIQ
                     PEEAETHALKNVILQALGAQNEIYPVPARLYPQRDDILLLCSDGLSNKLRGEDMLRIV
                     MENVDQLQMATALLVKEANERGGEDNITVILAKLTGSDLTEPTEEDVKLEIVNFNDIH
                     DTADQDTAEIV"
     gene            complement(1594..2082)
                     /locus_tag="VGB68_14235"
     CDS             complement(1594..2082)
                     /locus_tag="VGB68_14235"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEY0050447.1"
                     /translation="MEPYAWILWIVLGVILIIAETFTLGFVLFWFGIGALAAALAGFL
                     GLGTASQFFVFAVVSIALTVLSRTIFAKYYSHGDENAIKTGIDSLPGQIGTVSTASKG
                     ALGEGAVKVYGSTWTAFPEAGEEPLAEGEKVEVIRVQGSSIYVKRIGELPEWREDNSI
                     TN"
BASE COUNT          591 a          575 c          547 g          491 t
ORIGIN      
        1 tttcgtcgcg ctgaatgatg aagaagatgc caactggatt cagtttatcc agaacagcgt
       61 cggcgctatc ctgtcgcctt tcgattcgtt tctggttttg cgcggcacga aaaccttagc
      121 cgtgcggatg gaaaaacacg acgagaacgg gcgcgccgta gcgaattttt tagccgagca
      181 tccgaaagtc cgcaaagttt attatccggg actcgcctcg cacccgcagc acgagattgc
      241 caagcgccag cagaaaggct tcggcggaat ggtctcgttt gaaaccggct cgctggaaat
      301 ggcaaaaaag gttctggaaa gcgtcaagct atgcactttg gcggaaagtc tcggcggggt
      361 cgagagtttg atttcgcatc cggcgacgat gacgcacgct tccgttccgc aggaaaaacg
      421 cgaccagctc ggcattaccg acggtttggt gcgcgtctcc gtcgggattg aggacattga
      481 agatattatt gaagatttag accaagcgct tagttagtgc aaagtgctga gtactgagtg
      541 ctgagttttt atttctcact cagtactcag cactcagcac tcagcactta tttaattatg
      601 ttgattgttg agattcacgc cacgtcacac attggacgcg tacgccgcgg caatgaggac
      661 aattatcttt tgctgcaaat tgcagattca caaacctgga cgagttctca ggacccgagc
      721 gatttcatta tagaatcgca gcaattcgag gtggacgcga gcggcgtcat cttagccgtt
      781 tccgacggta tgggcggcgc gctcgccggc gaagttgcca gtaaaatggc ggtcgagact
      841 atcagcgaaa aaatcctgaa cgaagacccg gaatcgaccg tctcgccgca cagcacgggc
      901 gattctttaa ttgccaagct ctacgaggcg acgatttacg ccaatcatct gattcatcgc
      961 gaacagcgca gcaatcccga atacagcggt atgggcgcga cttttacggg catcggcgtg
     1021 acgcgcgaag ccgtcgacat cgtacaggtc ggcgacagcc gcgcatacct gattcgcaat
     1081 gcaaaaatct atcaaatcac caaagaccaa tcgctcgttc agcaattaat cgacgcacag
     1141 cagattcagc cggaagaagc ggaaacgcac gcgctgaaaa acgtaatcct gcaagcgctc
     1201 ggcgcgcaaa acgaaattta tcccgttccg gcgaggcttt acccgcagcg cgacgacatt
     1261 ctgcttttat gcagcgacgg cttgtcgaat aaactgcgcg gcgaagatat gctcagaatc
     1321 gtaatggaaa acgtcgacca gctgcagatg gcgacggctc tgctggtcaa agaagccaac
     1381 gaacgcggcg gcgaagataa tattacagtc attctcgcca aactaaccgg cagcgattta
     1441 accgagccga ccgaagaaga cgtcaaactc gaaatcgtca acttcaacga cattcacgac
     1501 accgcagacc aggacacggc ggaaatcgtt taaaattacg aattacgaat tacgaatgaa
     1561 ggaaatattc tttcaattcg taattcgtaa ttttcaattc gtaattgagt tatcttctct
     1621 ccattcggga agctcgccga ttctttttac ataaatcgaa gaaccctgaa ctcgaattac
     1681 ttcgactttt tcgccttcgg cgagcggttc ttcgccggct tccggaaaag ccgtccaggt
     1741 tgaaccgtaa actttgaccg cgccttcgcc gagagcgcct ttgcttgccg tcgagacggt
     1801 tccgatttgt ccgggcaaag agtcgattcc cgtttttatc gcgttctcgt cgccgtgcga
     1861 ataatatttc gcgaatatcg tgcgcgaaag cacggtcaga gcaatcgaaa caaccgcaaa
     1921 aacaaaaaat tgcgaggcag ttcccagccc gagaaaaccg gcaagcgccg ccgccagcgc
     1981 gccgatgccg aaccagaaca gcacgaaacc gagcgtaaag gtctcggcga taatcaaaat
     2041 cacgcctaaa acaatccaca aaatccaggc gtacggttcc atattgctcc tcgctgaaag
     2101 tctaaaacaa gcgaagacag tattgcaata attttatgat ttgtcattcg atttcgccat
     2161 ttgtcaccga atcaaagcct gtgataatct tttctgcgag tgct
//
LOCUS       DASWNE010000729         2146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_201183, whole genome shotgun sequence.
ACCESSION   DASWNE010000729 DASWNE010000000
VERSION     DASWNE010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201183"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VGB68_14240"
     CDS             <1..530
                     /locus_tag="VGB68_14240"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0050448.1"
                     /translation="RRIQVAFTEIAHAYEILKDEGSREIYDFKNRDKISASASGENTP
                     KAKAPANSAEAQAALAKESFDQGYSHLMDGDYEAALPLLARAVHMAPANARYHIFYGK
                     VLANGEKSVHKAESEMQTAIKLDPNNSVFRIMLAEFYIEVGLYRRAEGELQRLLATFP
                     NNKEAQDLLDSLPKK"
     gene            682..1413
                     /locus_tag="VGB68_14245"
     CDS             682..1413
                     /locus_tag="VGB68_14245"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY0050449.1"
                     /translation="MQTVQETNTDERLLKIAAEIDEFEGYAHPHATRIAALADEVAKK
                     FNLSPHDRYSLRQAALVHDIGEVLMNRDYIKANRVLRDDERVDMQRHTVIGEQEAAKR
                     GFSRAVQLLVRWHHEWWSGAGYPDALERDLIPLAARILRVCDTYAAMTDARPYSPAIS
                     EAEAKRYLTEWAGIEFDPSVVKAFLALEGLRELASFDLLEKNQSPDSLNANPADAAPA
                     ETKEDFSGGNAEQNSPYSQFWGDDN"
     gene            1479..>2146
                     /locus_tag="VGB68_14250"
     CDS             1479..>2146
                     /locus_tag="VGB68_14250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050450.1"
                     /translation="MIPSKTTSQGWLAFELNVLRRLEFTSAALPFTGEPNLGAYLKRG
                     NVRVLANDLTQSAFVKAVAAIQNNGEPLSETDVETVLEDAYVPRYRLQNPALRNWFNE
                     TDSWWFDNVRQNIERLPSLFTKATAMTLAMQAGNYLLSFTEETRELRQPLSTVFRRLR
                     SAYPKPVNNGQNNACHNKNAVEFIAENYTDLMFLRLPRAHNLSLRNAMGWTAWQEEWL
                     QAGDA"
BASE COUNT          522 a          587 c          588 g          449 t
ORIGIN      
        1 atcgccgcat tcaagtcgct tttacggaaa tcgctcacgc ttacgaaatc ttaaaagacg
       61 aaggctcgcg cgaaatttac gatttcaaaa accgcgacaa aatctcggct tcggcgtccg
      121 gcgaaaatac gccgaaagcc aaagcgcccg ccaattcagc cgaagcgcag gcggcgctgg
      181 cgaaagaaag cttcgaccag ggctacagcc atctgatgga cggagattat gaagcggcgc
      241 tgccgctgct ggcgcgcgcc gttcatatgg cgcccgctaa cgcccggtat cacatatttt
      301 acggaaaggt tctggcgaac ggcgaaaaat cggtgcacaa agccgaaagc gagatgcaga
      361 cggcaatcaa gctcgacccg aataactctg tctttcgcat tatgctcgcc gaattttaca
      421 tcgaagtcgg gctttatcgc cgcgccgaag gcgagctgca gagactgctg gcgacattcc
      481 cgaacaacaa agaagctcag gacttactcg acagtttgcc gaagaaatag taaaactcta
      541 gaaacgacct tttgatttcc ggtctgcgac ctttgagctt tcgagctttg accttctgtt
      601 ttaagttttt accgtcagcg tttaaactta aaacttcaaa gctcaaggct cacagaccgg
      661 aaatcgaaga cctatatttt tatgcaaact gttcaggaaa caaacacgga cgagcggctt
      721 ttgaaaatcg ccgccgagat tgacgaattc gaaggctacg cgcatcctca cgcgacgcgt
      781 atcgccgcgc tcgccgacga ggtggcgaaa aaattcaatc tctcgccgca cgaccgatat
      841 tctttgcggc aggcggcgct cgtccacgac atcggcgaag tgctgatgaa tcgcgattac
      901 atcaaggcaa accgcgtttt gcgcgacgac gagcgcgtcg atatgcagcg ccacacggtt
      961 atcggcgagc aggaagcggc gaaacgcggg ttttcgcgcg ccgttcaatt gctggtgcgc
     1021 tggcatcacg aatggtggag cggcgcgggc tacccggacg cgctggagcg cgatttgatt
     1081 ccgctcgccg cgcggatttt gcgcgtctgc gacacctacg cggcgatgac cgacgcgcgt
     1141 ccttacagcc cggcgatttc cgaagcggaa gcgaaaagat atctgaccga atgggcggga
     1201 atcgaattcg acccttcggt ggtcaaagcg tttctcgctc tggaaggctt gcgcgaattg
     1261 gcgtcgttcg accttttaga aaaaaatcag tcgccggact ctttaaatgc aaatccggcg
     1321 gacgccgcgc cggctgaaac gaaagaagat ttcagcggcg gtaatgccga acaaaattcg
     1381 ccttattcgc agttttgggg cgacgataat taaaacaaac gttactttga tttatcagaa
     1441 aagtctccgc aacaaaaagc gctgacatat tcgtgtatat gattccatca aaaacgacat
     1501 cgcagggctg gctcgcgttt gaactgaacg ttctgcggcg actggagttt acttccgccg
     1561 cgctgccttt taccggcgag ccgaatctgg gcgcttatct gaaacgcggc aacgtccgcg
     1621 tgctggcaaa cgatttgacg cagtcggctt tcgtcaaagc cgtcgccgcg attcaaaaca
     1681 acggcgagcc gctgtcggag acagacgtcg aaacggtttt ggaagacgct tatgttccgc
     1741 gctatcggct gcaaaacccg gctttaagaa actggtttaa cgagaccgat tcgtggtggt
     1801 tcgacaacgt gcgccagaat atcgagcggc tgccgtcgct gtttaccaaa gcgacggcga
     1861 tgacgctggc gatgcaggcg ggcaactatc ttttgtcttt cacggaggag acgcgcgagc
     1921 ttcgccagcc gctttcgacc gtctttcgcc gtctccgttc cgcgtatccc aagcccgtca
     1981 acaatggtca gaacaacgcc tgtcacaaca aaaatgccgt cgagtttatc gccgaaaact
     2041 acacggattt gatgtttctg cggctgccgc gcgcgcataa tctgagcctg cgcaacgcga
     2101 tgggctggac tgcctggcag gaagaatggc tgcaggcggg cgacgc
//
LOCUS       DASWNE010000730         3587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_24928, whole genome shotgun sequence.
ACCESSION   DASWNE010000730 DASWNE010000000
VERSION     DASWNE010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24928"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VGB68_14255"
     CDS             <1..224
                     /locus_tag="VGB68_14255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050451.1"
                     /translation="FSEKTVKGEIVLVIDRQENQRPKTEDRSPESLALRVEELEKDFD
                     HKSALKKAAKEFGLSRSEAYRMLISEKNR"
     gene            394..1038
                     /locus_tag="VGB68_14260"
     CDS             394..1038
                     /locus_tag="VGB68_14260"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY0050452.1"
                     /translation="MNKLIPAFCLTIISSVFAFAQTTDYKKSEFFVGYSNGQVDTGAN
                     SGDGVDEFFDDRLSFHGFNGSAVVNVNRYVGIKGDVSGTYKTNEFSFPVTTGTTTNTV
                     SFKTKSALYNFLGGVQVKDNASTGRLKPFAYALAGVGHGRIKVSNVSCPLGVDCSDFV
                     GDSETGLAGAFGGGLDIRVNDNIDFRVVKVDYNPIRFDSGTQHNIRLGIGVVFK"
     gene            complement(1185..2885)
                     /locus_tag="VGB68_14265"
     CDS             complement(1185..2885)
                     /locus_tag="VGB68_14265"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0050453.1"
                     /translation="MKKNLFAFLLLLTFILPSSALAQVAVKITPAERRAAEAITQEQL
                     KNYLYFIASDEMEGRNTPSRGLDLTAKFIALQLKQWGIKPAGDNGTYFQKINLRRDSI
                     DRANAFMEIGAQRFPYGEDFFKMRGSTNGTLSAPLVFVGDGWLVKSKNIDAHSNVDVR
                     GKIAVVQLYGAPTDPSLIILPKGVTRADLKPEARGTDWADPVTYAQQKGATGVVIVAS
                     PELQGFWKDVRQFLSGGGATRVEGLHEEPKTTGASIPVFLVSQTVGGALYEGEAGNPM
                     KSAAPVSAFEMNTAKKFGVAADSKAEVMTTQNVVAIIEGSDPILKSEMVAVGAHYDHV
                     GMNPNVPGDDKIFNGADDDGSGTTAVLAIAEALAKQPKKPKRSILFVWHAGEEKGLWG
                     SEYFVKYPTVDLKNVIAQLNIDMIGRSKKPGYQAPCDINPRPGQKPCNAELTGENEVY
                     VIGSEMMSSELGGITRGVNDSFLKMNYNYKYDDPKDENRFFFRSDHFHYATKGIPVAF
                     WFSGTHEDYHGVGDHADKIDYAKMEKVTRTIFMTLWELAEAKSRPKVDKQLPPELLQR
                     "
     gene            complement(3056..>3587)
                     /gene="lpxI"
                     /locus_tag="VGB68_14270"
     CDS             complement(3056..>3587)
                     /gene="lpxI"
                     /locus_tag="VGB68_14270"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF017989.4"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HEY0050454.1"
                     /translation="GLKQRNTDSLIGAVAAELAKDGIELIDSTFFIQDQLAQEGVLTK
                     RKPDSAEQGNIEYGLRIANEIARLDLGQTIVVRAKACVAIEAMEGTDACVKRAGELAK
                     GKLTVVKVAKPNQDMRFDVPVVGVPTIRTMSEAGATCLCLTAGKTLIFDKEEMLALAD
                     EKKIAVVAARAETNLI"
BASE COUNT          825 a          915 c          890 g          957 t
ORIGIN      
        1 ctttttcgga aaaaacggtc aaaggcgaaa ttgttctggt gatcgacagg caggaaaatc
       61 aaagaccgaa aaccgaagac cggagcccgg aatcgcttgc tttgcgggtt gaagaattag
      121 agaaggattt cgaccacaaa agcgctctga aaaaggcggc gaaggaattc ggcttgtcgc
      181 gttcggaagc gtatcgaatg ttaatttcgg aaaagaacag gtagaaaatt ttctcataaa
      241 tcaggcaact gttgccttta ttgtgacacc taaatattgt gacacctgaa aaagtgtcac
      301 aaacctaacg aatttagcag tatcaaattt gttaggcttt ttttattgct tcaagagcaa
      361 tttattaata aacaaggagt ctcaattttt attatgaaca aattaattcc ggcattttgc
      421 ctgactatta tttcaagcgt tttcgctttt gcgcaaacga ccgattacaa gaaatcggaa
      481 tttttcgtcg gttattcaaa cgggcaggtc gatacgggcg cgaacagcgg agacggtgtc
      541 gacgagtttt tcgacgaccg cttgagcttt cacggcttta acggttccgc cgtggttaat
      601 gtcaaccgtt acgtcggcat caagggcgac gtttcgggaa cttacaaaac caacgaattt
      661 tcctttccgg taacgaccgg aacgacgacg aacaccgttt cgttcaaaac gaaaagcgct
      721 ctgtataact ttttaggcgg cgtgcaggtc aaagataacg cttcgaccgg acgcctcaag
      781 ccctttgcct atgctctggc gggcgtcggt cacggcagaa ttaaagtctc gaacgtgtcc
      841 tgcccgctcg gagttgactg ctcggatttt gtcggcgatt cggaaaccgg tttagccggt
      901 gctttcggcg gcggcttgga catcagagtc aacgacaaca ttgatttccg cgtcgtcaaa
      961 gtcgactaca acccgattag attcgacagc ggaacgcagc acaacatccg cctcggcatc
     1021 ggcgttgttt tcaaataaac tttgctttca aagcaaaaat taaagggagc ttttatcaag
     1081 ctcccttttt tattgaatct gaatcgtgaa aaggcgaaaa agatggttta tcgaaaaata
     1141 acgaaaactt tttcactctt ttacattttc actctttcgc ttttttatct ttgcaaaagt
     1201 tccggcggaa gctgtttatc gactttcggt cgcgatttcg cttcggcaag ctcccataag
     1261 gtcataaaga ttgtccgcgt gactttttcc atcttcgcat agtcaatctt gtccgcgtgg
     1321 tcgccgacgc cgtgataatc ttcgtgcgtg ccgctgaacc agaacgccac cggaatgcct
     1381 ttcgtcgcgt aatggaaatg gtctgagcgg aaaaagaaac gattttcgtc tttcgggtcg
     1441 tcgtatttgt agttgtagtt cattttcaaa aacgaatcgt tgacgcccct ggtaatgccg
     1501 ccgagctcgc tgctcatcat ttccgagccg ataacgtaaa cttcattttc gcccgtcagc
     1561 tccgcgttgc acggcttctg tccgggtctc gggttgatgt cgcacggagc ttgatagccc
     1621 ggctttttgc ttcgaccaat catatcgatg ttcagttgag caatcacgtt tttcaggtcg
     1681 acggtcgggt atttgacaaa atattcgctg ccccacaagc ctttttcctc gcccgcgtgc
     1741 cagacaaaaa gtatcgagcg cttcggtttc ttcggctgtt tcgccagcgc ttcggcaatc
     1801 gccagcacgg cggtcgtgcc ggaaccgtcg tcgtccgcgc cgttaaagat tttgtcgtcg
     1861 ccgggaacat tcggattcat cccgacgtgg tcgtaatgcg cgccgacggc gaccatctcg
     1921 cttttcaaaa tcgggtctga gccttcgatg atggcgacca cgttttgcgt cgtcatcacc
     1981 tcggctttgc tgtccgccgc gacgccgaat tttttcgccg tattcatctc gaatgcggaa
     2041 acgggagcgg ctgatttcat cgggttgcca gcttcgcctt cgtacaaagc gccgccgacc
     2101 gtctgcgaaa cgagaaaaac ggggattgac gcgccggtgg ttttcggctc ttcgtgcagc
     2161 ccttcgactc gcgtcgcgcc gccgcccgac agaaattgcc tgacatcttt ccagaagccc
     2221 tgcagctccg gcgaagcgac gatgacaacg cccgtcgcgc ctttctgctg cgcgtaagtg
     2281 accgggtcag cccagtccgt tcccctggct tccggcttca aatcggcgcg cgtgacgcct
     2341 ttcggcagaa taatcagcga agggtcggtc ggcgcgccgt aaagttgaac gacggcgatt
     2401 ttgccgcgca cgtcgacatt cgaatgcgcg tcgatatttt ttgatttaac gagccagccg
     2461 tcgccgacaa aaacgagcgg cgcgctcagc gtgccgttcg tgctgccgcg catcttaaaa
     2521 aaatcctcgc cgtatggaaa acgctgcgcg ccgatttcca taaaagcatt ggcgcggtca
     2581 atcgaatcgc gccgcaaatt tattttttga aaataagtgc cgttgtcgcc cgccggtttg
     2641 atgccccatt gtttaagctg cagggcgata aatttggcgg ttaaatcaag cccgcgcgaa
     2701 ggcgtgttgc gaccttccat ttcgtcggaa gcgatgaaat acaggtagtt ttttaattgt
     2761 tcctgcgtaa tcgcttcggc ggcgcggcgt tccgccggtg taattttgac ggcaacctgc
     2821 gccagagcgg aagacggcag aatgaacgtc agcaaaagta aaaaagcaaa taaatttttc
     2881 ttcatttttt ctccgaaaag atttttcacc gcaggaacgc agagagagat atattcaaac
     2941 acgatgaaca ggataaatca gtttctaatc tgcgaaaatc tgcggttaat tctttcaaaa
     3001 atctccgtgt tttctctctc tgatgtgatg ataatttcct ttagttttac gtcggttaaa
     3061 ttagatttgt ttcggcgcgg gcggcgacca ccgcgatttt tttttcgtca gccagggcga
     3121 gcatttcttc tttatcgaaa atcagagttt ttcccgccgt caggcacaaa caggtcgcgc
     3181 ccgcttcaga cattgttcga atggtcggaa cgccgacgac tggaacgtcg aaacgcatat
     3241 cctgattcgg tttggcgact ttgacgaccg tcagtttgcc ttttgccaat tctccggcgc
     3301 gtttgacgca agcgtccgtg ccttccatcg cctcaatcgc cacgcaggct ttggcgcgca
     3361 cgacgatggt ttgtcccaaa tccagccgcg cgatttcatt ggcgattcgc aagccgtatt
     3421 cgatattgcc ttgttccgcg gaatcgggtt ttctttttgt cagaacgcct tcctgcgcga
     3481 gctggtcttg aataaaaaaa gtcgagtcga ttaattcgat tccgtccttg gcaagttccg
     3541 cggcgaccgc gccgataagc gaatcggtat ttctctgttt caagccg
//
LOCUS       DASWNE010000731         3209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_483477, whole genome shotgun sequence.
ACCESSION   DASWNE010000731 DASWNE010000000
VERSION     DASWNE010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483477"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1287)
                     /gene="gpmI"
                     /locus_tag="VGB68_14275"
     CDS             complement(<1..1287)
                     /gene="gpmI"
                     /locus_tag="VGB68_14275"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEY0050455.1"
                     /translation="MSSTKGRRPLALVILDGWGYSPRREGNAIALAHTPYYDEICARY
                     PKTLLEASGARVGLPEGVTGSSEVGHMNIGAGRIIHSEVAKIKQAIRSGEFFENRVLL
                     EAMRAAKNSALHLVGLLSDGDVHSSQENLFSLIRMAKKSGVQNVFVHAILDGRDVALR
                     TADIYVEALEIKLADIGLGTVATLCGRYYAMDKDQNWERTARAYTMLVHAEGERSRDA
                     VSAIRNSFLRGVADEFIQPIVLENPNGTPVTTVKNGDVVVFFNHRADRMRQLVKSLSG
                     SDADEFQTVGKPKIHMVCLTEYDRAFELPVAFPPAEESNVLAQVFARNGVLNCRLTET
                     EKYAHITYFFNGGVEVEHPCEQRVLVPSPKNSTHEFQPEMSSFKVTDKLLRGLEAGEN
                     DVFIVNIAAPDMVAHTGNLEKTIEAVQFVDSCLGGIV"
     gene            complement(1393..1872)
                     /locus_tag="VGB68_14280"
     CDS             complement(1393..1872)
                     /locus_tag="VGB68_14280"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0050456.1"
                     /translation="MPDLQGEIRDELKMSFHEWQNGEFIISTESARLQPEAIHKFLTE
                     ESYWARERTREQTATAIKNSLPFGVYKGENQIGFARVVTDYATFAYLGDVYILEQYRG
                     RGLSKWLMETIVNHPDLQNFRRWILATRDAHALYEKFGFAALKAPERWMERAAPNAY"
     gene            complement(1836..2048)
                     /locus_tag="VGB68_14285"
     CDS             complement(1836..2048)
                     /locus_tag="VGB68_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050457.1"
                     /translation="MFLIIGTKFISWGSQATAPMRCNQCNTVAAFKEKKGMRFITLFF
                     VIPIIPISGVKQLAECPTCKAKYEMN"
     gene            complement(2051..2428)
                     /locus_tag="VGB68_14290"
     CDS             complement(2051..2428)
                     /locus_tag="VGB68_14290"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY0050458.1"
                     /translation="MIEYLVDTNVLSKVFKNDFPVIRFVETLEAAIDTTIYIECLQGS
                     KSNYEKRVIEKYLIRFPLIPITAITSGRAIDLIRTYSNTYGLMIADALIAATALESDL
                     TIVTYNVDDFKFIQGLKYLKPAV"
     gene            complement(2425..2676)
                     /locus_tag="VGB68_14295"
     CDS             complement(2425..2676)
                     /locus_tag="VGB68_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050459.1"
                     /translation="MGQVVIEVPQDINLKFSVKSAEVVDEILQLIGKSPKQPETIQLT
                     YPYDLDDVDASEAYGIWADRKESAEEIAREIRDRNNGKI"
     gene            complement(2717..>3209)
                     /locus_tag="VGB68_14300"
     CDS             complement(2717..>3209)
                     /locus_tag="VGB68_14300"
                     /inference="COORDINATES: protein motif:HMM:NF005994.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEY0050460.1"
                     /translation="IGSSGAGKSTFARRLGEKTGLKVIHLDRLYWKPNWVETTDKDEW
                     KKVVREALAGEAWIIDGNYGGTMEMRLEKCDTVIFLDLPRALCVYRILKRVALYRPGA
                     RPDMAEGCDERFDWEFIKWVWNYPSRSKPKVEALLKRCENEKKIIRLKSRREIENFLL
                     NRS"
BASE COUNT          745 a          865 c          785 g          814 t
ORIGIN      
        1 cacgattccg cccagacagc tatcgacaaa ctgcacggct tcgatggttt tttccagatt
       61 ccccgtatgc gcgaccatat cgggcgcggc gatattgacg ataaatacgt cgttttcgcc
      121 cgcttcgagc ccgcgaagaa gcttgtcggt cactttaaaa ctgctcattt ccggctgaaa
      181 ttcgtgcgtc gaatttttcg gcgacggaac cagcacgcgc tgctcgcagg gatgttcgac
      241 ctcgacgccg ccgttaaaga aatacgtgat gtgcgcgtat ttttccgttt cggtcaggcg
      301 gcaattaagg acgccgttgc gcgcgaaaac ctgcgccagc acgttcgatt cctcggcggg
      361 cggaaaagcg acgggcaatt caaaagcgcg gtcgtattcg gtcaggcaaa ccatgtggat
      421 tttcggcttt ccgacggttt gaaattcgtc ggcgtctgag cccgaaagag atttgacaag
      481 ctggcgcata cggtcggcgc gatgattgaa aaagacgacg acatcgccgt ttttaacggt
      541 cgtgacaggc gttccgttcg ggttttccag aacaatcggc tggataaatt cgtcggcgac
      601 gccgcgcaaa aacgagttgc gaatcgccga cacggcgtcg cggctgcgct cgccttcggc
      661 gtgaacgagc atcgtgtaag cgcgggcggt gcgttcccag ttctggtctt tgtccatcgc
      721 gtaataacgc ccgcagagcg tggcgaccgt gcccaagccg atgtccgcca gcttgatttc
      781 cagagcttcg acgtaaatgt cggcggtgcg cagcgcaacg tcgcgcccgt ccaggatagc
      841 gtggacgaaa acgttttgaa cgccggattt tttcgccatc cggataagcg agaaaagatt
      901 ttcctgcgag gaatgaacgt cgccgtccga cagcagcccg accaggtgaa gcgccgagtt
      961 tttcgccgcc cgcatcgctt cgagcaaaac tctgttttca aaaaactcgc cgctgcgaat
     1021 agcttgtttg attttggcga cttccgaatg aataatgcgt cccgcgccga tattcatatg
     1081 accgacttcc gagctgcccg tgacgccttc gggcagaccg acgcgcgcgc ccgaggcttc
     1141 gagcagcgtt ttcggatatc tggcgcaaat ttcgtcgtaa tacggcgtgt gcgccagcgc
     1201 gatggcgttg ccttcgcgcc ggggcgaata gccccagccg tctaaaatta caagcgcgag
     1261 cggtcggcgt cctttagtgc tgctcatatc tgttccaaaa ctaaagctcc aatttccaag
     1321 tttaaatgtc cgcattggct tttaaccccg agccggaaat gccatgttcg gaaacatcat
     1381 aacacttttc gtttaataag cgttcggcgc ggctctctcc atccagcgtt cgggcgcttt
     1441 cagggcggcg aacccgaatt tttcgtaaag cgcgtgcgcg tcgcgcgtcg ccagaatcca
     1501 gcgccggaaa ttctgcaggt cgggatgatt gacaatcgtt tccatcagcc atttgctcaa
     1561 tccccgaccg cgatattgct ccagaatata aacatcgccg agataggcaa acgtcgcgta
     1621 atcggtgacg acgcgggcga atccgatttg attttcgcct ttatagacgc caaaaggcaa
     1681 ggaatttttt atcgccgtcg cggtctgctc gcgcgttcgc tcgcgcgccc agtaagattc
     1741 ttccgtcaga aatttgtgaa tcgcctccgg ctgcagccgc gcgctttcgg tgctgataat
     1801 aaattcgccg ttttgccatt cgtgaaagct catcttcagt tcatctcgta tttcgccttg
     1861 caggtcgggc attcggcgag ctgcttgacg ccgctgatcg ggataatcgg aatcacgaaa
     1921 aaaagtgtga taaaccgcat tccctttttc tctttgaacg ccgcgaccgt attgcattga
     1981 ttgcagcgca tcggagcggt cgcctgcgag ccccacgaaa tgaattttgt gccaatgatt
     2041 aagaacataa ttaaaccgcc ggtttcaaat attttaagcc ttgaataaat ttaaaatcat
     2101 ccacgttgta agtaacaatc gtcaaatcac tttcgagagc ggtcgccgca attaatgcgt
     2161 cggctatcat caagccatat gtattcgagt aagtacgaat caaatcaatc gcgcgaccgg
     2221 aagttatcgc agttattgga atcaaaggaa agcggattaa gtatttttca ataactcgct
     2281 tttcataatt agattttgag ccttgtaaac attcgatata aatcgttgta tcaatggcag
     2341 cttcgagggt ttccacaaat cgtatgaccg gaaaatcatt tttaaaaact ttggataaaa
     2401 catttgtatc aaccaaatat tcaatcatat tttcccgtta ttgcggtcac gaatctcccg
     2461 cgcgatttct tcggcagatt ctttgcggtc agcccagatt ccatacgcct ctgacgcatc
     2521 tacgtcgtcc aaatcatatg gataagttaa ctgaattgtt tccggctgct tcgggctttt
     2581 tccaatcaat tgcaaaatct catcaacaac ttcggcgctt ttgacgctga atttaagatt
     2641 aatatcctgc ggcacttcga taacgacttg tcccataaaa actctccctg ctcaaaaggc
     2701 attttaacac gaaaaatcac gaacggttca gcaggaaatt ctcgatttca cgcctcgatt
     2761 tcagacgaat tatctttttt tcattttcgc agcgtttcag cagcgcctcg actttcggtt
     2821 tcgagcgcga agggtaattc cagacccatt tgataaattc ccaatcgaat ctttcgtcgc
     2881 agccctccgc catatcggga cgcgcgcccg ggcgatagag cgcgactctt tttaaaatcc
     2941 ggtagacgca gagcgcgcgc ggcaaatcga gaaaaataac ggtgtcgcat ttttccagac
     3001 gcatctccat cgtgccgccg taattgccgt cgattatcca cgcctcgccc gccagagcct
     3061 cgcgcacgac tttcttccac tcatctttat cggtggtctc gacccagttc ggtttccagt
     3121 agagacggtc gaggtgaatg acttttaaac ccgtcttttc gcctaatctg cgagcaaagg
     3181 tagatttgcc cgcgccgctc gagccgatg
//
LOCUS       DASWNE010000732         1911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_94191, whole genome shotgun sequence.
ACCESSION   DASWNE010000732 DASWNE010000000
VERSION     DASWNE010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94191"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..602
                     /locus_tag="VGB68_14305"
     CDS             51..602
                     /locus_tag="VGB68_14305"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY0050461.1"
                     /translation="MFSRDIDENLKLILPLRQHAEELAAVVRGNLDQLKLWVPWATDA
                     YSVDSAREFIDENLKRLAESGCFALNLVWHGKIVGSIGFHNLDLNNKSAHVGYWLAKE
                     AQGKGLVTRCCRVLIDYLFDELKLNRVQINCNVENVKSRAIPERLGFELEGIHRQVEF
                     FDNRFGDWAVYAMLKQDWKKVDN"
     gene            670..>1911
                     /locus_tag="VGB68_14310"
     CDS             670..>1911
                     /locus_tag="VGB68_14310"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF002223.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEY0050462.1"
                     /translation="MIIVIIGAQWGDEGKGKVVDLLADRFDIVARYQGGHNAGHSVYV
                     GEKAFVLRLLPSGIIHEDSTCVLGNGMVIDPKAFFEEVDQMAEQGISITPERLKVSSR
                     AHLIMPYHRALDHTSEERLGNEKIGTTLRGIGPAYEDKAGRRGIRVSDALSPEVLRMR
                     IERNLEEANRIIVLYGQPPLYADQIFEEIAPLVERLRPFVTETSHFLAEARKEKKKIL
                     LEGAQATLLDVDHGTYPYVTSSNPTAGGASVGAGIPPHHITGVLGIVRTYATRVGEGP
                     FPTEMMDEEETVANLIRQRGNEYGSVTKRPRRCGWFDAVATRYAAELNGFDSLALTKL
                     DVLDALEEIKVCVGYEIDGERIDTFPAVSQDLRQIKPIYETLAGWQSETLGVTKIEDL
                     PEKARAYVEFLSNSIGVEIGLI"
BASE COUNT          469 a          471 c          557 g          414 t
ORIGIN      
        1 tcaatcccgc agataaaggc ggttgaaggt ttcgagtttc taataaaaat atgtttagcc
       61 gcgacatcga tgaaaacttg aaattaattt tgccgctccg gcagcacgcc gaagaattgg
      121 ctgccgtggt gcgcggaaat ctcgaccagt taaagctctg ggtgccgtgg gcgacggatg
      181 cttattcggt cgattcggcg cgcgaattta tcgacgagaa tttaaaacga ctggcggaaa
      241 gcggctgctt tgctctgaat ctggtctggc acgggaaaat tgtcgggagc atcggttttc
      301 ataatctgga tttgaacaat aaatccgctc acgtcggtta ctggctggcg aaagaagcgc
      361 agggcaaagg tctggtgacg cggtgctgcc gcgttttaat cgactatctt tttgacgagc
      421 tgaagctgaa tcgcgtacag attaactgca acgtcgaaaa tgtaaaaagc cgcgcgattc
      481 ccgaaagatt gggattcgaa ctggaaggaa tacaccggca ggtcgagttt ttcgacaatc
      541 gcttcggcga ttgggcggtt tacgcgatgc tgaaacagga ttggaaaaaa gttgataact
      601 gataactgat aaccgatagt ccggctcgtt tcactatcgg aaatcagtta tcaactttca
      661 cttaatttta tgataattgt aataatcggc gcgcaatggg gcgacgaagg aaaaggaaaa
      721 gttgtcgatt tgctggctga ccgcttcgat atcgtggcgc gttatcaggg cgggcacaat
      781 gccgggcatt cggtttacgt cggcgaaaaa gcgtttgtgc tcaggctttt gccttcggga
      841 attattcacg aagactcgac ctgcgtgctc ggcaacggaa tggtcatcga cccgaaagcg
      901 tttttcgagg aagtcgacca gatggcggag cagggaattt cgattacgcc cgaacgcctt
      961 aaggtttcga gccgcgctca tttgataatg ccgtatcatc gcgcgctcga ccacacttcg
     1021 gaagaacggc tcggcaacga aaaaatcggc acgaccttaa gaggcatcgg tccggcatac
     1081 gaagacaaag cgggcaggcg cggcattcgc gtctccgacg cgctttcgcc ggaagttttg
     1141 cgaatgcgca tcgagcgaaa tctggaagaa gcaaaccgca ttatcgtgct ttacggtcag
     1201 ccgccgcttt acgccgacca gattttcgaa gagattgccc cgctcgtcga gcgcctgcgc
     1261 ccgtttgtca cggaaacttc gcattttctc gccgaagcca gaaaagaaaa gaaaaaaatt
     1321 ctgctggaag gcgcgcaggc gacgcttctg gacgtcgacc acgggacgta tccttacgtt
     1381 acgtcctcga acccgacggc gggcggcgcg tcggtcggcg cgggcattcc gccgcatcat
     1441 atcacgggcg tcctgggaat cgtccgcact tacgcgacgc gcgtcggcga aggtccgttt
     1501 ccgaccgaga tgatggacga ggaggaaacc gttgcgaacc tgattcgaca gcgcggcaac
     1561 gaatacggct cggtgacgaa acgcccgcgc cgctgcggat ggttcgacgc ggtggcgacg
     1621 cgctacgcgg cggagcttaa cgggttcgat tcgctggctt tgacgaagct cgacgtgctg
     1681 gacgcgctgg aagaaatcaa ggtttgcgtc ggttacgaaa tcgacggcga aagaatcgac
     1741 acgtttccgg cggtctcgca ggatttgcgg caaatcaaac cgatttacga aacgctcgcg
     1801 ggctggcaaa gcgaaacgct cggcgttacg aaaatcgaag atttgccgga aaaagcgcgc
     1861 gcatacgtcg aattcttatc aaactcaatc ggcgtggaaa tcggcttaat c
//
LOCUS       DASWNE010000733         3495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_589412, whole genome shotgun sequence.
ACCESSION   DASWNE010000733 DASWNE010000000
VERSION     DASWNE010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589412"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VGB68_14315"
     CDS             <1..494
                     /locus_tag="VGB68_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050463.1"
                     /translation="YNPSVAANGNLYFSGLIEKDQKATAIFRSVYNNGVYGKAEKMPF
                     NDARYTFMDPSVSPDEKFIVFAFNGPGTMGSADIYIIRQKDGKWAEPVNLGASVNSAS
                     LENAPCLSPDGKTIYFTSMRTDVINFPKKKENTKELLKRLGSPLNGSRNIWQVDITSS
                     IND"
     gene            complement(831..2780)
                     /locus_tag="VGB68_14320"
     CDS             complement(831..2780)
                     /locus_tag="VGB68_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050464.1"
                     /translation="MNRVHTFAFRLLIAFLVLCSCFAAGTQAQTLESAPPLPPGMKGS
                     DANDPRAKLSPGMYDAGEAAMGIKHLLLLKKPDAFQLGSDNPDNPRVQKTLGQLGFGD
                     TSQFPKPLQLVFAQLAFANSDIAFQGNHLFQGNFYGVNIYDIANPAKTRLLTSLVCPG
                     GQGDVSVYRNLLFMSVEMPNGRLDCGPQGFPPEPPPVAGQENNQSLPAAQKERFRGVR
                     IFDISDIKNPKQVAAVQTCRGSHTHTLVVSPNDKNNVYIYVSGTSFVRPSEELAGCSD
                     APPDKDPNTALFRIEVIKVPLTAPQNAQIVSSPRVFMDTKTGALNGLNNGGTHDNNSL
                     QRPSETDQCHDITVYPSIGLAAGACSGNGILLDIKDPVNPKRVDAVNDPNYAYWHSAS
                     FSNDGKKVVFTDEWGGGLGARCRANDPNKWGANAVFRLTDNKLSFASYYKLPAAQGDS
                     ENCVAHNGSLVPVPGRDIKIQAWYQGGISVMDFTDPDHPVEIAYFDRGAIDPKVLVMG
                     GLWSAYWYNGYIYGSEIARGLDIFELTPTQYLTQNEIDAAKTVRLAELNVQNQEKIEW
                     SRQLVVAKAYVDQLERSQALPSVQIAALRQAIQKAESSNLNRRELGKLKSLAPSLEKS
                     AGLTKSGADAARLRDLAGILRRPAL"
     gene            complement(2791..3369)
                     /locus_tag="VGB68_14325"
     CDS             complement(2791..3369)
                     /locus_tag="VGB68_14325"
                     /inference="COORDINATES: protein motif:HMM:NF015658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEY0050465.1"
                     /translation="MVQPGAPGQPTRILPPTTRATLPPRSPKDVEFMQGMIMHHAQAV
                     EMTALVESHTENKDIRLLGARISKSQSDEIKFMKRWLESRGESVSPPVPKMPGMDMPG
                     HDMSSHHMTMPGMLTAKQMEALRKARGAEFDRLFLTGMIQHHNGALIMVKDLFDTAGA
                     GQDAELFNFASDVDSGQRAEIKIMQTMLGENP"
BASE COUNT          812 a          964 c          898 g          821 t
ORIGIN      
        1 tttacaatcc ttcggttgcg gcaaacggaa atctttattt ttcggggtta atagaaaaag
       61 accagaaagc taccgcgatt ttccgttcgg tttataataa cggagtttac ggtaaagccg
      121 aaaaaatgcc ttttaacgac gccagatata cgtttatgga cccgagcgtt tcgcccgacg
      181 aaaaattcat cgtttttgcc tttaacggtc ccggcacaat gggttcggct gatatttata
      241 tcatcaggca aaaagacggc aaatgggcgg agccggtaaa tctgggcgct tcggtcaatt
      301 cggcatcgct ggaaaatgcg ccgtgtctgt cgcccgacgg gaaaacgatt tattttacaa
      361 gcatgcggac ggatgtaata aatttcccga agaaaaaaga aaacacaaaa gaacttctaa
      421 agcggctcgg cagcccgtta aacggctcgc gtaatatttg gcaggtcgat attacttcgt
      481 cgataaatga ttgaagagga taaacagatt atcgaaaaac agtcgcaatt atgtgcaaaa
      541 acgcgcgaaa aaccggcagt ttacgagcga aatcccgagc ggatttaaaa tgaaaggcgc
      601 gtgagctgtt gatttcagat aagtataatc aaccgcttac gcgacttttt atttgccttt
      661 aacttaatca caagttgtca ctatgagcgt tttcacaagc tcaaaaagag ctatgttcat
      721 ttctcttaat gcaaactcgt ccaaagcctt tgattttcca aaaccggttt cctcaagcag
      781 cagcgacggc attttcgtct aaagcaattt cagctcgcgc ccgataagaa tcaaagcgcc
      841 gggcgacgca gaattccagc caggtcacgt aatcgggctg cgtctgcccc gctcttcgtc
      901 agtccggcgc tcttctccaa cgacggcgcg aggcttttga gttttccgag ctctcttcga
      961 ttcaggttcg aactctcggc tttttgaatc gcctgccgca gagctgcgat ttgaacggaa
     1021 ggcagagcct gcgaacgctc cagctgatcg acgtatgcct tcgccacgac taactgccgg
     1081 gaccactcaa ttttctcctg gttctgcaca ttcaattcag ccaaccgcac cgttttcgcg
     1141 gcgtcgattt cgttctgcgt caggtactgg gtcggcgtca gctcgaagat gtccaagccg
     1201 cgcgcgattt cagagccgta aatgtatcca ttgtaccagt aagcagacca gagtccgccc
     1261 atcacgagaa ccttcgggtc aatcgccccc cgatcgaaat aggcaatctc aacgggatgg
     1321 tcggggtcag taaagtccat aacggagatg ccgccctgat accaggcttg tatcttgatg
     1381 tcccgaccgg gaacgggaac gagcgagccg ttgtgcgcga cgcagttttc gctgtcgccc
     1441 tgcgcagccg gcagcttgta gtagctcgca aagctcagct tgttatccgt caagcggaaa
     1501 acggcattgg cgccccattt attcgggtcg ttcgcgcgac agcgcgcgcc cagcccgccg
     1561 ccccattcat cggtaaacac gacttttttc ccatcgttcg aaaacgaggc tgaatgccag
     1621 tatgcataat tcggatcgtt taccgcgtct actcgctttg gattgaccgg atctttgata
     1681 tccagcagaa ttccgtttcc cgagcaggcg ccggcggcga gtccgatcga cgggtacacg
     1741 gtaatatcat gacactggtc ggtttcggac ggtctctgca aactgttgtt gtcgtgcgtt
     1801 ccgccgttgt tcagcccgtt caaagcaccc gtcttggtat ccataaacac tctgggactc
     1861 gacactatct gagcgttttg cggtgccgtc aacggcacct tgatcacttc aatgcgaaag
     1921 agcgccgtat tcggatcctt gtccggcgga gcatccgaac acccagccag ctcttcgctc
     1981 ggacgaacaa aagaagttcc cgagacgtag atatagacgt tgttcttatc attcggggag
     2041 actaccagag tgtgcgtgtg cgagccgcga caggtttgca ccgccgccac ctgtttcgga
     2101 ttcttaatgt cgctgatatc gaaaatcctg actccgcgga aacgctcttt ttgcgctgcg
     2161 ggcaaagatt gattgttttc ttgtccggca acgggcggag gctcgggcgg aaaaccctgc
     2221 ggaccgcaat cgagcctgcc gttcggcatc tccaccgaca taaagagcag attcctgtaa
     2281 acggacacgt cgccctgccc gcccggacac accagcgaag tcaacaatct ggtcttcgcc
     2341 gggttggcga tgtcatagat gttgacgcca taaaagtttc cctgaaacag atggttgccc
     2401 tgaaacgcga tgtcggagtt ggcaaaagcg agttgagcaa aaaccagttg gagaggtttc
     2461 ggaaactgcg aagtgtcgcc gaagccgagc tgaccgagag tcttctgcac cctcggatta
     2521 tcaggattgt cggagccgag ctgaaacgcg tcgggctttt tgagcagcaa gaggtgcttt
     2581 atgcccatcg cggcttcacc cgcatcgtac attcccggcg acagtttggc gcgcgggtcg
     2641 ttggcatccg aacccttcat cccgggcggc agcggcggcg ccgattcaag agtttgcgcc
     2701 tgtgtcccgg cggcaaaaca cgagcacaaa acaaggaaag ctattagtaa acggaaagcg
     2761 aaagtgtgta cgcgattcat tgtttttcct ctacgggttt tcacccagca tagtttgcat
     2821 aatcttaatc tcagcgcgtt gaccgctgtc gacatcggat gcgaaattga acagctcggc
     2881 atcctgcccc gcgccagccg tatcgaacaa atctttgacc ataatcaacg cgccattgtg
     2941 gtgctgaatc attccggtca aaaacagacg atcgaactcc gcgcccctgg cttttctcaa
     3001 ggcttccatc tgctttgcgg taagcattcc gggcatcgtc atatgatgac tcgacatatc
     3061 gtgacccggc atatccatgc ccggcatttt cggcaccggc ggcgacacgg attctcctct
     3121 ggactcgagc caccgtttca taaactttat ttcgtctgac tgtgatttgc tgatgcgcgc
     3181 tccgagcagg cggatatctt tgttctccgt atgcgactca accagcgccg tcatctcaac
     3241 cgcttgcgcg tggtgcataa tcattccctg cataaactcc acatctttcg gcgagcgcgg
     3301 cggcagggtc gccctcgtcg tcggcggcag tattcgggtt ggctgccccg gcgcaccggg
     3361 ctgcaccacg accggcgact gagaatcaac ctgctgtgcg cagactggcg gcgataagaa
     3421 aaacgtgacg gcaccggcgg ctatcaaccc gcttcgcgat gccgcggcaa tgctcactaa
     3481 ccgcagcttt agcct
//
LOCUS       DASWNE010000734         7953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_272691, whole genome shotgun sequence.
ACCESSION   DASWNE010000734 DASWNE010000000
VERSION     DASWNE010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7953
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272691"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VGB68_14330"
     CDS             <1..849
                     /locus_tag="VGB68_14330"
                     /inference="COORDINATES: protein motif:HMM:TIGR03013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03013 family XrtA/PEP-CTERM system
                     glycosyltransferase"
                     /protein_id="HEY0050466.1"
                     /translation="KLPEARVLGTTANLEEIVHSEKIDRIVIAVRERRGAFPTEMLLR
                     MSLAGDVSIEECTSFYERITGQVHLDMLRPSWLIFAGRGRDTRLKTVFREAVHRTLGL
                     IGLVLSFPIALITAVLIKLDSKGPIFYRQERLGKNGRTFQVIKFRSMRTDAEANGKPV
                     WATADDDRATRIGKIIRVIRVDEIPQFWNIIRGEMSFVGPRPERPHFVSQLADEVPFY
                     EHRHLVAPGLTGWAQIKYPYGASVDDARQKLQFDLYYIKNQSLALDLVIVFETVKTVL
                     FGRGGR"
     gene            1007..1879
                     /locus_tag="VGB68_14335"
     CDS             1007..1879
                     /locus_tag="VGB68_14335"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEY0050467.1"
                     /translation="MKLVKFIRNTGLVLISGLVLSLGVSAQEAVQNQTTEATPTPQKR
                     ERVAKTEEVKPEDNSKVSPETKTETTDAPIAAPDDKRRESASEEEASILPYYNNYLKN
                     YRLGPEDIISVEVFAQCPNYCKTGIAIPPTAKISYPLIREGVFVGGKTVEQVADEITK
                     KLDEYIIDPKVTVTLERVGSARYSVLGKVAAPGVRLMTRRVSIYEAIVESGGVLKNGD
                     RKRVMIYRQSEKGLTQIPVDLQAIERGKAEMVYLVPGDQVFIPDAGFKLSVSNVFKVL
                     EKASLVRILFGVPF"
     gene            1985..3007
                     /locus_tag="VGB68_14340"
     CDS             1985..3007
                     /locus_tag="VGB68_14340"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEY0050468.1"
                     /translation="MFRKMNPVKKISFLFLFLFCFQIAEAGWTKQNSGTFAWLHSVYF
                     LDENKGWIVGSRGTFLSTEDGGSSWKQASRFTEDRVRDVYFTDEQNGWILCERDIYNL
                     GSAAPSYLMKTTDGGERWERIEFGEGRDRIARMFFTKSGYGYAVGESGVFFAMRDDKK
                     TWKKTPVPSRYLLLDGKFPDDSHGAIVGGGRTILFTEDAGLTWNKATVFGKSESKLNS
                     IFFVNRRTGWTVGSNGKIYATTNAGKIWREQISNVDKDLLDVYFTSPTEGWAVGDRGA
                     ILHTTTAGSLWLPVETNARHKLEKVFFAGRKGFAVGFGGTILVYDSTRSQSTVKSKPQ
                     MLKRNQ"
     gene            3154..3226
                     /locus_tag="VGB68_14345"
     tRNA            3154..3226
                     /locus_tag="VGB68_14345"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3187..3189,aa:Val,seq:tac)
     gene            3268..3344
                     /locus_tag="VGB68_14350"
     tRNA            3268..3344
                     /locus_tag="VGB68_14350"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3302..3304,aa:Asp,seq:gtc)
     gene            complement(3464..4078)
                     /locus_tag="VGB68_14355"
     CDS             complement(3464..4078)
                     /locus_tag="VGB68_14355"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY0050469.1"
                     /translation="MMQPNSRIYLEQLLAEMIEQPARQAEIARVIEDVFGQNKAVLVL
                     DMSGFCRTARDHSIVMVLSMIYQMRELLKPCVEAEGGQLIKAEADNLFCLFDTVGDAL
                     GGCRQIQNSLMEVNRQMAEHCRLNVSMGIGYGRILNIEDKDIFGEEVNLASKLGEDIA
                     GKGEILLTKNAFLQLQETESCVREETVEISGFSLRYHIVQGLLL"
     gene            4909..6564
                     /locus_tag="VGB68_14360"
     CDS             4909..6564
                     /locus_tag="VGB68_14360"
                     /inference="COORDINATES: protein motif:HMM:NF028240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HEY0050470.1"
                     /translation="MEEIKRQLREQQKEIEKLQILTAQQARMIEKLQNNSVQTTQMPT
                     ASTQASNNPASAQTETIESRLSKVEEQSKKTIEAIAGNRFGTLGFSGDLRMQYDSLYG
                     LLNNAANVENPAITGNELSSRQRIRYRLRFAVRGKIGNDVFTGAYAANGEKRTDKEFE
                     WGMRLTAGSLSNPISPNPVLTDFFSRKPIALDQVYVSWRPRPLPGLRLVAGKFEPTWM
                     RTEMIIDNDLQVEGVSEVYSRDIKNSFLKNITFSAWQLPMLERGTSFIRNADGTVNIQ
                     ESGRSGRDLALYGAQFQARLALSAKTNLTLAATNLHYANTNSINPIQVFGNNLQLPVT
                     VTIPGTATAPSQTVTGVATISRDLLISGNGNLGLAAASNNAVNRDGRLASGFNLIDFL
                     AQIEFKQFKHNPLTFIFDYVRNTQTRDVIVAGSSGNNVFLPNNENEGVWAELRLQNLR
                     RKRGSDFNAPVGGDFLFSYTFLQIEKDAVLTPFNWDDLVQPSDIRAHRLYLSYTVDPR
                     VAFNFTGLFNQRPNGLLGPFVTTPAGSLNRNTNRLQIDTVFRF"
     gene            complement(6631..7710)
                     /locus_tag="VGB68_14365"
     CDS             complement(6631..7710)
                     /locus_tag="VGB68_14365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050471.1"
                     /translation="MINFQNTGRQKSFQTLTAIILIAVCAVAAQAHVKWFFDYDVSQP
                     PTPIGEVLDGTFVKMFLVSVAACYLFFLADRYIYEKGILSEFDKKLKLFDNAANYIMR
                     VAAGVFFLALFGWWALGIGESFYLTPELKTASPFVPWLQLALALAVISRYTIFLTGLG
                     VFALYVAAASNYGIFHMLDYMIFLGIGYYLTVSQTKNKGLLKSGFVVLFACTGLTLIW
                     AAVEKFAYSQWTIAVLADKPHVTMGMSPPIFMKVSGFIEFFVTFILLGAVSVVGRLIA
                     LGFMSIFVLAVFEFGTLDAVGHLMIVAILWVLIVRGPTDARDMLVLPDKSLFTEAYFM
                     TGLYILAFVMVFILYYGIHHYAYGS"
BASE COUNT         2101 a         1953 c         1984 g         1915 t
ORIGIN      
        1 aaattgcccg aagcgcgcgt tctcggcaca acggcgaatt tggaagaaat cgttcacagc
       61 gaaaaaatcg accgcatcgt gattgccgtg cgcgagcggc gcggcgcgtt tccgaccgag
      121 atgcttttgc ggatgagcct ggcgggcgac gtctccatcg aagaatgcac ttcgttttat
      181 gaaagaatca ccgggcaggt tcatctggat atgctgcgcc cttcgtggct gatttttgcc
      241 ggacgcggac gcgacacgcg cctgaagacg gttttccgcg aggcggttca tcgcactctg
      301 gggttgatag gattggtttt atcgtttccg attgccttga ttacggcggt tttaatcaag
      361 ctcgattcga aaggtccgat tttttaccgg caggagcgat taggaaaaaa cggcagaacg
      421 tttcaagtca tcaagtttcg ctcgatgcgc accgacgccg aggcgaacgg caaacccgtc
      481 tgggcgacgg ctgacgacga ccgcgcgacg cgcatcggaa aaataatccg cgtcatccgc
      541 gtcgacgaaa ttccgcagtt ctggaacatt atcaggggcg agatgagctt tgtcggaccg
      601 cgaccggaac gcccgcactt cgtttcgcaa ttggcggacg aagtcccgtt ttacgagcat
      661 cgccatctgg tcgcgccggg cttgaccggc tgggcgcaaa tcaaatatcc gtacggcgca
      721 tcggtcgacg acgcgcgcca gaaactgcaa ttcgatttgt attacatcaa aaatcaaagc
      781 ctcgcgctcg atttggtgat tgtctttgaa acggtgaaga ccgttttgtt cgggcgcggc
      841 gggagatgat tcaggattca ggattcaaaa ttcagaattc aggattcaga attacaaatc
      901 ttgaatcttg aatcttgaat cttgaatcct gaatcctgaa tcctgaatcc taaaatttgg
      961 aattttgaat cagataggcg tttttacctt tgacggagaa agaaacatga agttagttaa
     1021 gtttattcgc aatacgggtt tggtcttgat aagcggtctg gttttgtcgc tcggcgtttc
     1081 ggcgcaggaa gcggtgcaga atcagacgac ggaagcgacg ccgacgccgc aaaagcgcga
     1141 gcgcgtggcg aaaaccgaag aagtcaagcc cgaagataat tcaaaggttt ctcccgaaac
     1201 aaaaacggag acgaccgacg cgccaatcgc agcgccggac gacaaacgcc gcgaatcggc
     1261 gtccgaggaa gaagcctcga ttctgccgta ttacaacaat tacctgaaaa attaccggct
     1321 cggacccgaa gatataattt ccgtcgaggt tttcgcgcaa tgcccgaatt actgcaaaac
     1381 gggcatcgcc attccgccga cggcgaaaat ttcctatccg ctgattcgcg aaggcgtctt
     1441 tgtcggcggc aaaacggtcg agcaggtggc tgacgaaatc acgaaaaaac tcgacgaata
     1501 catcatcgac ccgaaagtca cggtcacgct cgaaagagtc ggctcggctc gctacagcgt
     1561 gctcggcaag gtcgccgctc cgggcgtgcg gctgatgacg cggcgcgtca gcatttacga
     1621 agcgattgtc gaatccggcg gcgttctgaa aaacggcgac cgcaagcgcg tgatgattta
     1681 tcgccagtcg gaaaaaggct tgacgcaaat cccggtcgat ttgcaggcta tcgagcgcgg
     1741 caaagccgag atggtttatc tggttcccgg cgaccaggtt tttattcccg acgccggatt
     1801 taagctgagc gtcagcaacg tcttcaaagt tttggaaaag gcgagcctgg tgagaatttt
     1861 attcggcgtc ccgttttaat cgggaaaatt caaacaggat ggacaggatg aacaggataa
     1921 aaatcaagag taaatgcttt gaatttttct tatcctgcct atcctgtcca tcctgttaaa
     1981 ttttatgttt agaaagatga atccggtgaa gaaaatttca tttttattcc tgtttctgtt
     2041 ctgttttcag attgccgaag ccggttggac aaaacaaaat tccggcacct tcgcgtggct
     2101 tcactcggtt tattttctgg acgaaaacaa aggctggatt gtcggcagcc ggggaacttt
     2161 tctctcgacc gaagacggcg gaagctcctg gaaacaagcg agtcgtttta ccgaagaccg
     2221 cgtccgcgac gtttatttca ccgacgagca aaacggctgg attttgtgcg agagagatat
     2281 ttataatctc ggctcggctg cgccgtcgta tctgatgaaa acgacggacg gcggcgaaag
     2341 atgggagaga atcgagttcg gcgaaggtcg cgaccgcatc gcgcggatgt ttttcaccaa
     2401 aagcggttac ggctacgcgg tcggcgaatc gggcgtgttt ttcgcgatgc gggacgataa
     2461 gaaaacgtgg aaaaaaacgc cggttccgag tcgttatctg ctgctcgacg gaaagtttcc
     2521 ggacgattcg cacggcgcaa tcgtcggcgg cgggcgcacg attcttttta ccgaggacgc
     2581 cggtctgacg tggaacaaag cgaccgtttt cggaaaatcg gaatcgaagc tgaattcgat
     2641 tttcttcgtc aaccggcgta ccggctggac ggtcggctca aacggaaaaa tttacgcgac
     2701 cacgaacgcc ggaaaaatct ggcgcgagca gatttcaaac gtcgataaag atttgctgga
     2761 cgtttatttc accagcccga ccgaaggctg ggcggtcggc gaccggggcg cgattctgca
     2821 tacgacgacg gctggcagcc tctggcttcc cgtcgaaaca aacgcgcgcc acaagctcga
     2881 aaaagttttc ttcgccggac gaaaaggctt cgccgtcggc tttggcggaa cgattctggt
     2941 ttacgattcg acccgctcgc aaagcactgt aaaatcaaag ccgcaaatgc tgaaaagaaa
     3001 tcaataaagg ttttcggctc tcgcctgact tgacagcccg tgcgaaattt tcgtagcata
     3061 acagttcgct gtttggtgga agacagcgat gttttttttg acaaaaattg ggcaaaccga
     3121 tagcgtgccg tcaaaagaat atttgaaaat ttcgggcatg tacgtcaatt ggtagacagc
     3181 ctcccttaca aggaggaagt taggggttcg agtcccttcg tgcccactaa aagaatttca
     3241 aatctcaaat ttgagatttc agaatgcgga gtcgtagttc agttggttag aacgccagcc
     3301 tgtcacgttg gaggtcgcgg gttcgagtcc cgtcggctcc gccattaata tctttagaat
     3361 caacagattc ggaaccgtca ttggtggcgg ttttttgttt ttctttccct gtacccgaat
     3421 ttgtatcctc cggctcttcc ggcagctttc cggcaattag tccttataaa agcaagccct
     3481 gtacgatatg ataacgcaga gaaaatcctg aaatttcaac ggtttcctcc cggacgcagg
     3541 attcggtttc ctgtaattgg agaaaagcat ttttagttaa caaaatctct cctttaccgg
     3601 ctatgtcttc gcctaattta gaagccagat tgacctcctc gccgaatatg tccttatctt
     3661 caatattcag aatacgtccg tagccgattc ccatcgatac attcagacgg caatgctccg
     3721 ccatttgccg gttgacctcc atcaaactat tttgaatttg ccgacatccg ccgagcgcat
     3781 cgccgacggt atcaaacaag cagaaaagat tatcggcttc ggctttaatc aactgcccgc
     3841 cctctgcctc gacacaaggt ttcagcagct cccgcatttg ataaatcata gacaaaacca
     3901 taacgatgct gtggtcgcgc gcggttcggc aaaagccgct catatctaaa accaggacgg
     3961 ctttattttg tccgaaaacg tcttcgataa cccgagcgat ttccgcctgc cgcgccggtt
     4021 gttcaatcat ctccgcgaga agctgctcaa gataaattcg cgagttgggt tgcatcatat
     4081 cggctttcct gtcggttgtt tgaaagggcg gaaaatttaa cgctccttat cgtaaaaaca
     4141 ttaccgtctt tcgtttactt cgaatacttt accggggcat cgctcaaact caagccttaa
     4201 ttgcctccgt ccgtaaattg acgaacgagc ttatcccgga ttgcctgaaa gtcgaataaa
     4261 tcaataaatt tatcgaaacg gcaattatcc gcgcaaagtt ttgacaagtc caaacggcat
     4321 acgtattata aatgaaattt tcggtcgccc ggtcataaaa ctttatttct gcttggaaaa
     4381 agccttctta atgttcgatt tagattgacg gttgcagaga tatcgccaat ggcattaatt
     4441 aaatttttct ttcaggctct gaacaaaatt gatttgtatc gcaaggctgc gcgacaataa
     4501 tccagctttg ctgttttagg atataggtaa taactttggt caactataaa taacaaacgg
     4561 catttcgaaa aaatgaaagc cgcattgcgc gtttttcccg atgcctgccg gttttattga
     4621 gctttctgcg gtcgcgtcct tgcgttatca atgtgaaatt aattttttac atttctcttt
     4681 cttttatttg tcgataaata aacgagagag aaatgacggc aatcagataa ttgtttcgac
     4741 aaacataaat tgtcgcactt ttttatgaat aataatctgc gaatattata tctattacta
     4801 tctttttgtt ttctcaacgc cgccgtcccg gcacaatcgc ttacctctgt cgaggaggct
     4861 ggcaacactg cctcgacaac tgcttctaat tccacacgaa tttccgagat ggaagagata
     4921 aaaagacagc tgcgagaaca gcaaaaagaa attgaaaagt tacagatatt gactgctcag
     4981 caagccagga tgattgaaaa gctgcaaaac aactctgtgc agacgactca aatgccgacc
     5041 gcctcgacgc aagcctcgaa caatcccgct tccgcgcaga ccgaaacaat cgaatcccgt
     5101 ttgagcaagg ttgaagaaca atcgaaaaag acgattgaag cgatcgccgg aaaccgattc
     5161 ggcacgctcg gtttcagcgg cgatttgcga atgcagtatg attcgcttta cggtctgctc
     5221 aacaacgcgg caaacgtcga aaatccggca attaccggca acgaattgtc ttcgcgccag
     5281 cggattcgat atcgtctgcg tttcgccgtc agaggaaaaa tcggcaacga cgtattcacg
     5341 ggcgcatatg ccgcaaacgg cgaaaagaga acggataaag aatttgaatg gggaatgcgc
     5401 ctgaccgccg gcagtttatc caatcccatt tcgcccaatc cggtattgac cgattttttt
     5461 tcgcgcaagc cgattgcgct cgaccaggtt tatgtttcgt ggcgtccgcg cccgcttccc
     5521 ggtttgcggc tcgtcgccgg taaattcgaa ccgacgtgga tgcgcaccga gatgattatc
     5581 gacaacgatt tgcaggtcga aggcgtcagc gaagtttatt cgcgcgacat taagaattca
     5641 tttctgaaaa acatcacctt cagcgcgtgg cagctgccga tgctcgaacg cggaacaagc
     5701 tttatcagaa acgccgacgg gactgtgaat atccaagaat ccggacgttc cggaagagat
     5761 ttggcgcttt atggagcgca gtttcaagcc agactggcgc tttccgcgaa aacgaatctg
     5821 acgctggcgg cgacaaatct gcattacgca aatacgaatt cgataaatcc gatacaggtt
     5881 ttcggcaata atctgcagct gcccgtcacg gtcacgattc ccggcacggc gaccgcgccg
     5941 agtcagacgg taacgggcgt cgcgacgatt tcgcgcgatt tactaatttc cggcaatggc
     6001 aatttaggat tggcggcggc gagcaataac gccgtaaacc gcgacgggcg gctcgcgtcg
     6061 ggctttaatc tgattgattt tttggcgcaa atcgaattta agcaattcaa acataatccg
     6121 ttgacgttta ttttcgatta cgtgagaaac actcaaacgc gcgacgtaat agtcgccggc
     6181 tcgtccggca acaatgtttt tctgccaaac aatgaaaacg aaggcgtctg ggctgaattg
     6241 cgccttcaga atttgcgcag aaaacgggga agcgatttta acgcgccggt cggcggcgat
     6301 tttctgttca gttatacgtt tttgcaaatc gaaaaagatg ccgtcttaac gccgttcaac
     6361 tgggacgatt tggttcagcc gtccgacatt cgggcgcatc gcctgtattt gagctacacg
     6421 gttgacccgc gcgtcgcttt taattttacg ggtttgttta atcagcgacc gaacggtttg
     6481 ctcggtccgt tcgtgacgac tccggcgggc tcgctgaacc gcaataccaa tcgcttgcaa
     6541 atcgatacgg tattcaggtt ctaagttttt atagttttat aagccttgta aaaagtatcg
     6601 cccgaaaact gcggtgaaat gtgatttaaa tcaggaaccg taggcgtaat ggtgaattcc
     6661 gtaatagaga ataaagacca tcacaaacgc gagaatataa agcccggtca taaaataggc
     6721 ttcggtaaaa agcgatttat cgggcagcac gagcatatcg cgggcatcgg tcggaccgcg
     6781 cacgatcaaa acccacaaaa tagcgacgat cattaaatga ccgaccgcgt cgagcgttcc
     6841 gaactcgaaa accgcgagca cgaaaatcga cataaagccg agcgcaatca atcgcccgac
     6901 gaccgacacc gcgccgagca gaatgaacgt gacgaaaaat tcgataaagc ccgaaacctt
     6961 cataaagatc ggcggcgaca ttcccatcgt cacgtgcggt ttatccgcca gaaccgcaat
     7021 cgtccactgc gaataggcga atttttcgac cgccgcccag atcagcgtca gtcccgtgca
     7081 ggcgaaaagc acgacgaaac ccgatttcag caagcctttg tttttcgtct gcgaaacggt
     7141 cagataataa ccgataccga gaaaaatcat atagtcgagc atatgaaaaa tcccgtagtt
     7201 cgaagcggcg gcgacgtaaa gcgcgaaaac gccgagtccg gtcagaaaaa tggtatatcg
     7261 cgaaatgacc gccagagcca acgccagctg aagccacgga acgaacggcg aagcggtttt
     7321 cagctcgggc gtcagataaa aactctcccc gattcccaga gcccaccagc cgaaaagcgc
     7381 gagaaaaaaa actccggcgg cgacgcgcat aatgtagttg gcggcattgt cgaaaagctt
     7441 cagcttttta tcgaattcgg ataaaatccc tttttcgtaa atgtatcggt cggcgagaaa
     7501 aaacaggtag cacgcggcga ccgaaacgag aaacatttta acgaaagttc cgtccagaac
     7561 ttcgccgatc ggcgtcggcg gctggctcac gtcgtaatcg aaaaaccatt tgacgtgcgc
     7621 ctgcgccgcg accgcgcaga cggctattaa aatgattgcc gtcaaggttt gaaaagattt
     7681 ttgtctgccg gtattttgaa aatttatcaa gttgtttctc caaagctcgg aatttcgcct
     7741 caactttttc ctgtaatatg cgcccaaatc attacagacg ttgatgcgcg cgagagttac
     7801 tttcgttttg ataatttctg ctcccggatt aataaaatta tctgatttcc atctgattta
     7861 tgaacgtcgc ggcggaatct tccggtcgaa cgagcatttt ttgcctgccg gctgggacaa
     7921 atagacaaaa acaacgattt gccgcctgaa att
//
LOCUS       DASWNE010000735         3390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_555669, whole genome shotgun sequence.
ACCESSION   DASWNE010000735 DASWNE010000000
VERSION     DASWNE010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555669"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..1511)
                     /locus_tag="VGB68_14370"
     CDS             complement(171..1511)
                     /locus_tag="VGB68_14370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0050472.1"
                     /translation="MSTNLPLPTHPPQIGLSKGFAFSTAEDRERVLIVDDSSTVRKIF
                     ARSLSPRYACVEAASVSEAFAQLAETEFALVITDVLMPGLSGVELLRKIIETYPDTAV
                     IMVSGVNQPQRALDAVRLGAFDYLFKPCDLSVLELTVERALERRSLLLNARQYKRDLE
                     VRNNELVRGKAQLQRLQAQIVHSEKMASLGQLAAGIAHELNNPVGFVYGNLDILNQCI
                     NDLIKLLGYYDGANLPEDIAAGAASIKKEIDYEASIEDLSSIICDCRDGAERVRDIVQ
                     NLRTFSRLDEAEFKKTDIHEGIDSTVRLLSRYFSADNITLVRDYGKLPPIETFSGQLN
                     QVWMNLLVNAAQAVSAGGEVRITTRADGESVIISIGDTGGGIAPEHLNRIFDPFFTTK
                     PVGEGTGLGLSISFGIVERHGGKITVKTVLKQGTTFTVTLPVHIKAPSSAAAEF"
     gene            complement(1775..2386)
                     /locus_tag="VGB68_14375"
     CDS             complement(1775..2386)
                     /locus_tag="VGB68_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050473.1"
                     /translation="MKNLLLLLLASFLLGSNALAQSDIKMRTEYGSENIDLQSVLYFE
                     NIGLEKMIFSGSDLKDKDFQISIKEFVSGKQTRTEVVFDSKEDAYFKIKSDRFIFRVL
                     TKVTSENTVKFDFQFNGFSKQKEYKVAANQKAFSLKDFRGSRPEISMPLNTNTYILIY
                     MMPYLRKDGSTAYCEVAQSGISPEDFGKKYAIPTYFLIDIKFQ"
     gene            complement(2551..>3390)
                     /locus_tag="VGB68_14380"
     CDS             complement(2551..>3390)
                     /locus_tag="VGB68_14380"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY0050474.1"
                     /translation="GKYTHLFHKTLTETIDNRKQKYRFDLKIEIAQVEPPGETVPKLT
                     VKYYDSTSRRGTLNIADRRIVFGLTGTAGIYNGQYNNLYFDLNGDDKLDTQTRYSPEK
                     YKIPEKYVNIGEATYEFSVDRYGDNLTLKPLAEKMPARVDLSPGNSAPEIAFTDLEGK
                     ARRLSDFRGKVVLLDFWGLWCAPCVAEAPNLAAAYKRLKEKGFEIVSFDKGDTVENLR
                     KFIDKTQMNWTHSQLDEPLSRLYRIDSYPTYFLLDKEGKIISNTMRPGEELYKKIEEI
                     LGN"
BASE COUNT          848 a          796 c          813 g          933 t
ORIGIN      
        1 acccggagca gttcgagcgc ttctgcgccg gacgttgcgc cgacgacttc gtattgacgg
       61 cgaaaaaggc gttcaagcac ccgtaaatta gccgcttcat catcaacaat aagaattttg
      121 tagctcatat aatttattaa cctttcgttt tatatcgtga tttgtgacga ttaaaattca
      181 gccgccgcag aggacggcgc tttgatatgg acgggcaggg taacggtgaa agtcgttccc
      241 tgtttcaaga cggttttcac agtgatttta ccgccgtgac gctcgacgat gccaaaagag
      301 atggaaagcc ctaagccggt gccctcgccg accggtttgg tggtgaaaaa cgggtcgaag
      361 atgcggttca gatgctcggg tgcgatgccg ccgcccgtat cgccgatgga aataatcacg
      421 gattctccgt ccgcgcgcgt ggttatgcga acttcgccgc cggcgctgac cgcctgcgcg
      481 gcattgacca gcaaattcat ccacacctga ttgagctgcc cggaaaaagt ctctatcggc
      541 ggaagtttcc cgtaatcgcg aacgagcgta atattgtcgg cgctgaaata acgcgacagc
      601 agcctgacgg ttgaatcgat gccttcgtga atgtcggttt ttttaaactc cgcttcgtcg
      661 agcctggaaa aggtgcgcag attttggacg atgtcgcgga cgcgctcggc tccgtcgcgg
      721 caatcgcaga taatagagct taaatcttca atcgaggctt cgtaatcaat ttctttttta
      781 atcgaggctg cgccggcagc gatgtcttcc ggcagattcg ctccgtcata atagccgagc
      841 agttttatta aatcattgat gcattgatta agaatgtcca gatttccata gacgaaaccg
      901 accggattgt taagctcgtg ggcgatgccg gcggcaagct gaccgagaga agccattttt
      961 tcgctgtgaa caatctgcgc ctgaagccgc tgaagctgag ccttgccgcg caccaattcg
     1021 ttattgcgca cttccaaatc ccgcttgtat tgccgcgcgt tgagcagcag gctgcgcctt
     1081 tccagcgcgc gttcgacggt cagctccagg acgctcaaat cacaaggctt aaataaataa
     1141 tcaaacgcgc cgaggcgcac cgcatccagc gcgcgctgcg gttgattaac tcccgaaacc
     1201 ataataacgg cggtatccgg ataggtttcg attattttgc gcagcagctc gacgccggac
     1261 agccccggca tcagaacatc ggtaataacc agagcaaatt cggtttcagc cagctgcgcg
     1321 aatgcctcgc tgaccgaagc cgcttcgaca caggcgtagc gcggcgaaag gcttcgcgca
     1381 aatattttcc ggaccgtgct cgaatcatcg acaatcagaa cacgttctct gtcttcagcc
     1441 gttgaaaagg cgaatccttt cgataaaccg atttgtggcg gatgagttgg taaaggcaag
     1501 tttgtggaca ttgcttttta aatgttgttg cagtgaaaag tcgaaacaaa aaatcatagc
     1561 cgtaattatc atatcatctc ttttaaaaat tgttaatact ttttaaacga cctgagcaat
     1621 aatgaagaaa acgctcttat taacgatgag atttcgagcc cggcaaatac aaaaagcccg
     1681 atgcgcggtc ggcggcttcg aatttgaccg aaaatcgaca gccggtttta caataaattg
     1741 tttggcggca atatcgtttc gccggagcaa acatttattg aaattttatg tctatcaaaa
     1801 agtaggtcgg aattgcgtat ttctttccga aatcttcggg gcttatgccc gactgagcga
     1861 cttcgcaata agctgtagag ccgtctttcc ttaagtacgg catcatataa atcaaaatgt
     1921 aagtgtttgt gtttaaaggc attgaaatct caggtcggct tcctcgaaaa tctttcagag
     1981 aaaacgcttt ttgattggcg gcaaccttgt attctttttg tttggaaaat ccgttgaact
     2041 gaaagtcgaa tttgaccgta ttttccgaag tgactttggt caaaacgcga aagataaatc
     2101 tatcgctttt gattttaaaa taagcatctt ctttcgaatc gaaaacgact tccgttctcg
     2161 tttgttttcc gctcacaaat tctttgatgg aaatctgaaa atctttatct ttcaaatcgc
     2221 tgccggaaaa tatcattttc tccagaccga tgttttcaaa ataaagaacg ctctgcaaat
     2281 caatattttc cgagccgtat tcagtccgca tttttatatc ggattgagcg agagcgtttg
     2341 agcccagcaa aaacgaagcc agtaaaagta aaagtaaatt tttcatggtt ttatattttt
     2401 caagttatta ttttcccgct tttaacgagc cgtcgccgca tcaagagcga ttggacgaat
     2461 acaaatgatt agtttcgcat ttcacgaaaa gccttggatt tttatcactt acggctgcgc
     2521 cgtccgggcg aaatccatct gcggagatga ttaatttccg agaatttctt cgattttctt
     2581 atacagttct tcgcccgggc gcatcgtgtt ggaaataatc tttccttctt tatcaagcaa
     2641 aaaatacgtc gggtagctat caatgcgata cagccgcgag agcggttcat ccagttgaga
     2701 atgcgtccaa ttcatctgtg ttttgtcgat aaatttccgt aaattttcaa ccgtatcgcc
     2761 tttatcgaaa ctgacgattt caaagccctt ctcttttagt cttttgtaag cggctgccag
     2821 gttcggcgct tcggcgacgc acggcgcgca ccacaagccc cagaaatcca acaggacgac
     2881 tttgccgcga aaatcggaaa ggcggcgcgc tttgccttcg aggtcggtaa aggcgatttc
     2941 gggagcggaa ttgcccggcg ataaatcgac gcgggcaggc atcttttcgg cgagcggttt
     3001 cagcgtcaaa ttgtcgccgt aacggtcgac tgaaaattcg taggtcgcct ctccgatatt
     3061 gacgtatttt tccgggattt tatatttttc cggcgaatat cgcgtttggg tgtcgagctt
     3121 gtcgtcgccg tttaaatcaa agtaaagatt attgtattga ccgttgtaaa tgcccgccgt
     3181 cccggtcaag ccgaaaacga ttcggcggtc ggcgatattc agcgttccgc gccgcgaggt
     3241 ggaatcgtaa tatttgacgg taagcttcgg gacggtttct cccggaggct cgacttgcgc
     3301 aatttcgatt tttaaatcga accggtattt ttgttttcga ttatcgattg tttcggtcag
     3361 cgttttatga aacaggtgcg tgtatttgcc
//
LOCUS       DASWNE010000736         3434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_94325, whole genome shotgun sequence.
ACCESSION   DASWNE010000736 DASWNE010000000
VERSION     DASWNE010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94325"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VGB68_14385"
     CDS             <1..222
                     /locus_tag="VGB68_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050475.1"
                     /translation="KIKLDGAVKFEEPPPTEKPADTPPTPIDATTTSTESAPVAESNS
                     GGKKEKAPGKIKKKVKAKAEETVKEIIDN"
     gene            325..786
                     /locus_tag="VGB68_14390"
     CDS             325..786
                     /locus_tag="VGB68_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050476.1"
                     /translation="MRKFICYGFLILTFCAFGAAQETKKDDRKIKKLPGKIKGEIVIK
                     DATGNNLGEFKCANLRVFASRMQRDPYTKQKWMRESLAAGNFSARRCSFLITDVPVDS
                     GVFSIFAQLESAPCEQKSMKANASFPMEMKKGGETLTYNITVYELRCFVIK"
     gene            799..1206
                     /locus_tag="VGB68_14395"
     CDS             799..1206
                     /locus_tag="VGB68_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050477.1"
                     /translation="MNNQRIRQICGILICIVVLSALPAFSKLEIKEKTAVKKRIQKLI
                     YCPESVNFQLQTPAEWYAGLYDRILLKFESSATDGKVIYCEYQVNNAQYKNHVTLTRD
                     VPKNFRCRSDGLKSKRFTCEPNAPPIKIPKKNN"
     gene            complement(1299..>3434)
                     /gene="alaS"
                     /locus_tag="VGB68_14400"
     CDS             complement(1299..>3434)
                     /gene="alaS"
                     /locus_tag="VGB68_14400"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEY0050478.1"
                     /translation="PENPEKNRADLVNADEGDLTMEIWNLVFMQYNRVETAKGVYKLE
                     LLPAPSVDTGGGLERMTVVMEGVHSNYETSLVKPIINFIAELAGVKYEYESTEGFAMR
                     VVADHARATAFSITDGILPGNEGRNYVLRKIMRRAIYHGREHLGFKDLFFNKVCDFVV
                     EHMREAYPELEIQREFIGKMVRLEEERFGNTMTVGLQKLDEFFAKKDEADFIEFAKLY
                     DTFGTPRDLVRIKLEEKGIELGEDDFNQRFDTALQEIQSQSAVGKTERKSAISPIYHE
                     IAEKVGANQFHGYETTQVEEAKIVALLKDDRQIEELAEGDEGLIVLSETPFYAESGGQ
                     VGDTGFLMNETTKVKVQDTFSPVAGLILHKSKVEKGELKVGDAATAMVDTEKRDATRR
                     NHTATHLVHAALKEVVGTHVKQAGSVVAPNYLRFDFTHYQALSEAEIKEIEDLVNRYI
                     LQNEPVQTNIMAIEEAMRTGAVALFGEKYGADVRVLSVGEGVFSKELCGGTHVRATGD
                     IGSFKITSDEAIASGVRRIRAITGFDAFERFREDEKLIEKSLAHLRTQRDHLPSAIEK
                     LQEELKRTRREVEELKMKIATGAIGTASANGDEAKEINGVKVLAKTVEGLDKGGMRHL
                     SDTLLAKLKSGVVVLARSEEDKVSFIVRVSDDLTGKVKAGKIVQEIAPIVGGRGGGKP
                     DMAEGGGTDASKLNDALQASYGVVEQMLS"
BASE COUNT          840 a          911 c          822 g          861 t
ORIGIN      
        1 aaaatcaagc tcgacggcgc ggtcaaattc gaagagccgc cgccgaccga aaaaccggct
       61 gatacgccgc cgacgccgat tgacgcgacc acgacttcta ccgaaagcgc gccggtagcc
      121 gaatctaata gcggcggcaa aaaagaaaaa gcgcccggta aaatcaagaa aaaagtgaaa
      181 gcaaaggcgg aggaaaccgt gaaggaaatt atcgacaatt agacggcttc gagtttcgcc
      241 ggcagaaacg tttgtcaaaa aaaactgatg gaaccggcag gcgaaactca aaatttaccg
      301 gataaaacac aaaggagaga aagaatgcga aaatttatct gttacggttt tctgattttg
      361 actttctgcg ctttcggcgc ggcgcaggaa acaaaaaaag acgatagaaa aataaaaaaa
      421 ctgccgggca aaatcaaagg cgaaatcgtt atcaaggacg cgaccggcaa taatctgggc
      481 gagttcaagt gcgctaacct gcgggttttc gcctcgcgga tgcagcgtga cccttataca
      541 aaacaaaagt ggatgcgcga gagcctcgcc gccggaaatt tttcggcgcg gcgctgctct
      601 tttttaatta ccgacgtgcc ggtagattcc ggagtattca gcattttcgc ccaactggaa
      661 tccgcgccct gcgaacagaa gagtatgaag gcaaacgctt cgtttccgat ggagatgaaa
      721 aaaggcggcg aaaccctgac ttacaacatc acggtttatg aactcaggtg tttcgtcatc
      781 aaataaaggc tcggcgatat gaacaatcaa agaatcagac aaatctgcgg aatattaata
      841 tgtattgtgg ttttatcggc gcttccggcg ttttccaagc tcgaaattaa agagaagaca
      901 gcggtaaaaa agcgaattca aaaattaatt tattgtcccg aatcggttaa tttccagctt
      961 caaacgccag ccgaatggta tgcgggcttg tatgaccgga tacttttgaa atttgaaagc
     1021 tccgcgaccg acggcaaagt tatttactgc gaataccagg taaataacgc gcaatataaa
     1081 aatcacgtga cgctgacccg cgacgttccg aaaaatttca gatgccggag cgacggctta
     1141 aaatcgaaaa gattcacctg cgagccaaac gcgccgccga ttaaaattcc gaaaaagaat
     1201 aattagaggc tcggcgaaac gacttttcaa gcaaaaacag ctcggctgca ggatattttt
     1261 atctcgtcgc cgtgctgttt tatgcagtta gattttcgct agctcagcat ttgctcgacc
     1321 acgccgtagc ttgcctgcaa agcgtcgttt aatttgctcg cgtcggttcc gccgccttcc
     1381 gccatatcgg gtttgccgcc gccgcgaccg ccgacaatcg gcgcgatttc ctgcacgatt
     1441 tttccggctt ttacttttcc ggtcaaatcg tcggagacgc gcacgataaa cgaaactttg
     1501 tcttcttcgc tgcgagccag aacgacgacg ccggatttca gttttgccag cagcgtgtcc
     1561 gacaaatgac gcattccgcc tttgtccaag ccttccacgg ttttcgccag cactttgacg
     1621 ccgttgattt ctttggcttc gtcgccgttt gccgaagccg tcccgatggc gccggtcgca
     1681 atcttcattt tcaattcttc gacttcgcgc cgggtgcgtt tcaattcttc ctgcaatttc
     1741 tcaatcgcgc tgggcagatg gtcgcgctgc gtgcgcaaat gagcgagcga tttttcgatt
     1801 aatttttcgt cctcgcggaa acgctcgaac gcgtcgaagc cggtaatcgc gcggatgcgg
     1861 cgcacgcccg aagcaatcgc ttcgtcagaa gtgattttaa agctgccgat gtcgcccgtc
     1921 gcgcgcacgt gcgttccgcc gcaaagctct ttcgagaaaa cgccttcgcc gacgctcaaa
     1981 acgcgaacgt ccgcgccgta tttttcgccg aaaagcgcga ccgcgcccgt ccgcatcgct
     2041 tcttcaatcg ccattatatt cgtctgcacc ggctcgttct gcaaaatata acggttgacc
     2101 aaatcttcga tttccttgat ttccgcctcg ctcaaagctt gataatgcgt gaaatcgaat
     2161 cgcagataat taggcgcgac gaccgaaccg gcttgcttga cgtgcgttcc gacgacttcc
     2221 ttcaaagccg cgtgaaccag gtgcgtcgcc gtgtgatttc ttctggtcgc atcgcgtttt
     2281 tccgtgtcga ccatcgccgt cgccgcgtcg ccgactttga gctcgccttt ttctactttc
     2341 gatttatgca aaatcaaacc cgcgacgggc gagaaagtat cctgcacctt cactttcgtc
     2401 gtttcattca tcaaaaatcc ggtgtcgccg acctgcccgc ccgattcggc gtaaaagggc
     2461 gtttcgctca aaacgattaa gccttcgtcg ccttccgcca gttcttcgat ttgccggtcg
     2521 tctttgagca gagcgacaat tttcgcttct tctacctgcg tcgtttcgta accgtgaaat
     2581 tgattcgcgc cgactttttc agcgatttcg tgataaatcg ggctaattgc cgatttacgc
     2641 tcggttttgc cgaccgcgct ctgcgattga atttcctgca aagccgtgtc gaatctttgg
     2701 ttaaaatcgt cttcgcccag ctcgattcct ttttcttcca atttgattcg caccaaatcg
     2761 cgcggcgttc cgaaagtgtc gtaaagtttg gcaaattcga taaaatcggc ttcatctttt
     2821 ttcgcgaaaa actcgtcgag cttttgcagt ccgaccgtca tcgtattgcc gaatctttct
     2881 tcttcgagcc gcaccatttt gccgataaat tcgcgctgaa tttcgagttc gggataagcc
     2941 tcgcgcatat gttcgacgac aaaatcgcag actttgttga aaaacaaatc tttaaaaccg
     3001 agatgctcgc gcccgtgata aatcgcgcgg cgcataattt tcctgagaac gtaatttctg
     3061 ccttcgttgc cgggcaaaat tccgtcggtg attgaaaaag cggtcgcgcg ggcgtggtcg
     3121 gcgacgacgc gcatcgcaaa gccttcggtc gattcgtatt cgtattttac gcccgccagt
     3181 tcggcgataa aattgataat cggtttgacg agcgaagttt cataatttga atgaacgcct
     3241 tccatcacga ccgtcattcg ttcgagcccg ccgcccgtat cgaccgacgg cgcgggcaaa
     3301 agctctaatt tgtaaacgcc tttcgccgtc tcgacgcggt tgtattgcat aaagacgaga
     3361 ttccagattt ccatcgtcaa atcgccttcg tcagcgttga ccaaatcggc acggtttttt
     3421 tcgggatttt ccgg
//
LOCUS       DASWNE010000737         1617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_336442, whole genome shotgun sequence.
ACCESSION   DASWNE010000737 DASWNE010000000
VERSION     DASWNE010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336442"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..889)
                     /locus_tag="VGB68_14405"
     CDS             complement(143..889)
                     /locus_tag="VGB68_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050479.1"
                     /translation="MKIFIIFLALITIFPALAVSGFAQHTAAGVTDTYQIGNKQIVIP
                     SPNGFLEAGSQIPVIKQHFTATESGGNDFLAAHIPAEHLQRLKKGEIFDLTFYTKVSV
                     SKRLKTLDSTEADFAQLVATFKKGFPALSDPNGKEMKSTLKSISENLSNLNKTETTYT
                     LSQPLNVGLIEDSRNAYGTILLTQIKLTSGGKEIVRMMLGGISAIRVREKIVFIYTYR
                     VYEKEQDIAEIQQFSKQWLTRIIAANSNAK"
     gene            complement(996..1316)
                     /locus_tag="VGB68_14410"
     CDS             complement(996..1316)
                     /locus_tag="VGB68_14410"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY0050480.1"
                     /translation="MQEITATELKRRMDAGDDIQLIDVRQPNEYAFAKIEGAALIPLG
                     ELMNRIGEIDENRETVVHCHAGGRSARAIDAMQRAGFKGELKNLKGGITAWSDEVDPS
                     VPKY"
BASE COUNT          410 a          360 c          396 g          451 t
ORIGIN      
        1 aaaagcccgc gctcgcctgc cgcacaaagg ttgcggaagg aatgaaaatc gtcaggctga
       61 ccggcgaaat tgatttaagc gaaatgcttg acgccgccgg tgacaaatag attccttttt
      121 tgataatcgg gcagccggaa aattatttcg cgttgctgtt tgcggcaata atccgggtca
      181 gccattgttt tgaaaattgc tgaatctcgg cgatgtcctg ttctttttca tacactctgt
      241 aggtatagat aaacactatt ttttcgcgca cccgaatcgc cgaaatgccg ccgagcatca
      301 ttcgcacgat ttctttgccg ccgcttgtca gctttatttg cgtcagcaaa atcgttccgt
      361 acgcgttgcg gctgtcttcg ataagaccga cgttgagcgg ttgcgaaaga gtgtaggtgg
      421 tttcggtttt gttcagattc gaaaggtttt ccgaaataga ctttaaggtg gatttcattt
      481 ccttcccgtt cgggtcggaa agcgccggaa agcctttttt aaatgtggcg accaactggg
      541 cgaaatcggc ttcggtggag tctaaagttt ttaatctttt tgaaacggaa acttttgtgt
      601 aaaaggttaa atcgaaaatt tcgccttttt ttaaacgctg aagatgctcc gccggaatgt
      661 gcgcggctaa aaaatcattt cccccggatt cggtggcggt aaaatgctgt ttaattacgg
      721 gaatttgaga accggcttcg aggaaaccgt tcggcgacgg aatgacaatt tgtttattgc
      781 cgatttgata ggtgtccgtt acgcccgcgg cggtgtgttg ggcgaaacct gaaacagcga
      841 gcgccggaaa aattgtaatc aaagctagaa aaattataaa aatcttcatt aaatccttac
      901 ccgtttgccc ggcggcggga gaagtatccg aattaaatta accggtatta aaaattaaat
      961 taaccggtat taaaacacaa aaccgaagca ttgtcttaat atttcggaac gctcgggtca
     1021 acttcgtcgg accaggcggt gatgccgcct tttagatttt tcagctcgcc cttaaatccg
     1081 gcgcgctgca tcgcgtcgat cgcccgcgcg ctgcgcccgc ccgcgtgaca atgcacgacg
     1141 gtttcgcggt tctcgtcaat ctcgccgatt cgattcatca attcgccgag cggaatcagc
     1201 gcagcgcctt cgattttggc aaaggcgtat tcgttcggct ggcgcacgtc tatcaattga
     1261 atgtcgtcgc ccgcgtccat tcttcgtttc agctcggtgg cggtaatttc ctgcatattt
     1321 ttttcctctc aagtcgtttt aagtttttac aattctatac tttctgtaag gtatcggaca
     1381 aactgtgtcg tccgtagccg atataatttt tgttcacaaa agattatcgc aggctgaaag
     1441 atttgcttta aagttgccgg aaatgccgca ggtctttacg aatttcgtga ggaatgcggc
     1501 ataacgactt ttgtagtatt aaagccgaaa aaaagaaccg tgatggaaag gaaataataa
     1561 aggcggcacg agcgcgacag caaagcaatg tctctgaaag atacgctgaa agacgac
//
LOCUS       DASWNE010000738         2725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_272914, whole genome shotgun sequence.
ACCESSION   DASWNE010000738 DASWNE010000000
VERSION     DASWNE010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272914"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2725)
                     /locus_tag="VGB68_14415"
     CDS             complement(<1..>2725)
                     /locus_tag="VGB68_14415"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0050481.1"
                     /translation="ISAQSGDKLPPIKYTEFRLKNGLRVIFHEDRSTPIVAVNVWYHV
                     GSKNETVGKTGFAHLFEHMMFQGSKNWNTDYFKPLDAAGANVNGSTNADRTNYYEVLP
                     SNFLELALFLEADRMGNLLDAMTQEKLDNQRDVVKNERRQRYDNQPYGTAIEKIMDII
                     YPKGHPYNWTTIGSLDDLTAASQDDVKGFFRQYYVPNNASLVVAGDFNPKQARAWVQK
                     YFGAIKKGGEITRPKLPVPAMQGVIRKEFEDAVQSPRVYMVWHTVPQGNKDEAALDIL
                     SSIFAAGRGARLQSKLVYDKQIAQDVIAGHPTREIAGTFQIAVTARPNGPKDVEEMRT
                     NPGKFLAAIEEQVNIEIERIKKEPPTMEEVSRAVNRIEAQTIFGLQTVLGKADAMNSN
                     ATFYGKPDRFQQQLDEYRKVTPADVVRVANAYLNANHLVMNFVPRKDKTTSSQTNNAA
                     NTPTSKSSKEKPAVDQTKNLPKAGADPKFNLPTIEKQKLSNGLEVWLVRQPELPIVSM
                     NMVFKSGGTIDPSGKSGLASFTSSLLNSGTKTRSAVDISNQLSDIGANLGTGSNWDSA
                     NVSMQTLTKNLDKALDIYSDVLLNPTFPDKELDTLRRRSLFALQQRRDNPNAIAGVVY
                     NALLYGRNHPYGNSLGGDEISIKSFKQSDLENFYKTYYRPNNAVLIVVGDATMDTLKP
                     RLETAFANWKPAEVPSMKVPEAAMFDRPGIYVVDKPGAAQSIISIGQVGVPRDNPDYF
                     AIQVMNDMLGGSFSSRLNMNLREDKGYTYGAGSGFGFRRGAGPFTASAGVQTAVTKES
                     VLEFMKELNGIRGERPVTQEELETSKQSLIRSFPQGFETNGQIAGQLSTLATYDLPDS
                     YFNEYFSKINAVTLADINRVANKYLTPDKMAIVIVGDRSAIEPRLREIEIWG"
BASE COUNT          552 a          732 c          736 g          705 t
ORIGIN      
        1 gccccagatt tcgatttctc tgagtcgcgg ttcaatcgcg cttctatcgc cgacgataac
       61 aatcgccatt ttatccggcg tcagatattt attggcgacg cggttaatat cggcgagcgt
      121 cacggcattg attttcgaga aatattcgtt gaaatacgaa tccggcaaat cgtatgtggc
      181 gagcgtggaa agctgaccgg cgatttgccc gttggtttca aaaccctgcg ggaagctgcg
      241 aatcaggctt tgcttgctgg tttcgagttc ttcctgcgtg accggtcttt cgccgcgaat
      301 gccgttgagt tctttcataa actcaaggac ggattctttc gtgacggcag tctgcacgcc
      361 agccgaagcc gtgaaaggtc ccgcgccgcg ccggaagccg aagccgctgc ccgcaccgta
      421 agtgtagcct ttgtcctcgc gcagattcat attcaggcgc gacgagaaag agccgccgag
      481 catatcgttc atcacctgaa tcgcgaaata atcgggatta tcgcgcggga cgccgacctg
      541 accgatagag ataatcgact gcgccgcgcc gggtttgtcc accacataaa ttccgggtct
      601 atcaaacatc gccgcttccg gcactttcat cgacggcact tccgccggct tccaattggc
      661 aaaagcggtt tccagcctcg gcttgagcgt gtccatcgtc gcgtcgccga cgacaatcaa
      721 aaccgcattg ttcgggcgat aataggtttt atagaaattc tccaaatccg attgtttaaa
      781 ggatttaatc gagatttcat cgccgcccag agagttgccg tacggatgat ttctgccgta
      841 aagcaaagcg ttgtagacga cgccggcaat cgcgttcgga ttgtcgcggc gctgctgcaa
      901 ggcgaacagc gagcgtcggc gcagcgtatc cagttcctta tcgggaaacg tcgggttaag
      961 caacacgtcc gagtaaatat ccagagcttt gtcgaggttt ttcgtcagag tctgcatcga
     1021 aacgtttgcc gaatcccagt ttgagcccgt tcccagattc gcgccgatgt cggacaattg
     1081 attggaaata tcgaccgccg aacgggtttt cgttccgctg tttaaaagac tcgacgtaaa
     1141 cgaagccaag ccggattttc cgctcgggtc aatcgttccg ccggatttga aaaccatatt
     1201 catcgaaacg atcggcaatt ccggctggcg cacgagccag acttccaaac cgttcgaaag
     1261 cttctgtttt tcaatcgtcg gcagattgaa tttcgggtcg gctccggctt tcggcagatt
     1321 ttttgtctgg tcgaccgccg gtttttcttt cgacgatttt gaagtcggcg tgtttgccgc
     1381 attgtttgtc tgcgacgatg tcgttttgtc tttgcgcggg acgaaattca tcaccagatg
     1441 attggcgttc aggtaagcgt tggcgacgcg cacgacatcg gctggcgtca ccttgcgata
     1501 ttcgtccaac tgttgctgaa atcggtcggg cttgccgtaa aacgtcgcgt tgctgttcat
     1561 cgcatcggct ttgccgagaa cggtttgcag tccgaaaatc gtctgcgctt cgatgcgatt
     1621 gacggcgcgc gaaacttctt ccatcgtcgg cggttctttc ttgatgcgct cgatttcgat
     1681 attgacctgt tcctcgattg ccgccaggaa tttgcctgga ttagtgcgca tttcctcgac
     1741 gtcttttggt ccgttcgggc gcgcggtcac ggctatctga aacgttccgg caatctctct
     1801 ggtcgggtgc ccggcgataa cgtcctgcgc gatttgctta tcataaacca gcttgctttg
     1861 caggcgcgct ccgcgtccgg cggcgaaaat cgagcttaag atatcgagcg cggcttcatc
     1921 tttattgccc tgcggaacgg tgtgccagac catataaacg cgcggcgact gcacggcgtc
     1981 ttcaaattcc ttgcggataa cgccctgcat cgccggcacg ggcagcttcg ggcgcgtaat
     2041 ctcgccgcct tttttaatcg cgccgaaata tttctgtacc cacgcgcgcg cctgtttcgg
     2101 attaaagtct ccggcgacga ccagcgatgc gttattcggc acgtaatatt gacggaaaaa
     2161 tcctttgacg tcgtcctgcg aagcggcggt caaatcgtcg agcgagccga tggtcgtcca
     2221 gttatacgga tgacctttcg ggtaaataat atccattatt ttttcgattg ccgtgccgta
     2281 aggctggtta tcgtagcgct ggcgacgctc gtttttcacg acgtcgcgct ggttgtcgag
     2341 cttttcctgc gtcatcgcgt cgagcaggtt gcccatccgg tcagcttcca gaaacagcgc
     2401 cagttccaga aaattcgaag gaaggacttc gtaataattc gtgcggtcgg cgttggtcga
     2461 gccgttgacg ttggctcccg cggcgtcgag cggtttgaaa taatcggtgt tccagttttt
     2521 cgagccttga aacatcatat gctcgaaaag atgcgcgaaa ccggttttgc cgacggtctc
     2581 gtttttcgag ccgacgtgat accagacgtt aacggcgaca atcggcgtcg agcggtcttc
     2641 gtgaaaaatc acgcgcagac cgtttttcaa acgaaactcc gtgtatttaa tcggcggcag
     2701 cttgtcgccg ctctgtgctg aaatc
//
LOCUS       DASWNE010000739         2638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_589736, whole genome shotgun sequence.
ACCESSION   DASWNE010000739 DASWNE010000000
VERSION     DASWNE010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589736"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /gene="xth"
                     /locus_tag="VGB68_14420"
     CDS             <1..670
                     /gene="xth"
                     /locus_tag="VGB68_14420"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY0050482.1"
                     /translation="TKCTDDKFPAGVFLEAGYNSAFLGQKSYNGVAILSPSEIGDVQM
                     NFPDDDRDAPKRLIAATINGVRVVNTYIPNGTELWTDKFTFKLDWLQRLRRFFDETCD
                     KNSDVLLCGDFNVAPDERDVWNVGFWEGKLHFSKPERAAIHHVKQWGFVDVFRQLNGD
                     EKLYSWWNYREGAFFKNQGLRIDHIWTSRSLSEKCTACWIDRKPRGAERPSDHAPVVA
                     EFEI"
     gene            831..1571
                     /gene="pgl"
                     /locus_tag="VGB68_14425"
     CDS             831..1571
                     /gene="pgl"
                     /locus_tag="VGB68_14425"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEY0050483.1"
                     /translation="MKASTKNCEVEIFPNVAALNQAAARSIVEIAAKALQANDKFSIA
                     LAGGSTPEALYKMLASGEFKNRIDWTKTLVFFGDERCVSPENAESNYRMANAALLARV
                     PLPPENVFRLRGETEPEKAAFEYEETIKKTLGENARFDLILLGMGDDGHTASLFPRTE
                     ALSAAGKLVAANYVEKLKANRLTLTFRAINSARNVLFLVAGAGKAGMMKKVLSVETID
                     LPSSLIKPENGSCRWFLDPEAAALIEIL"
     gene            complement(1675..>2638)
                     /locus_tag="VGB68_14430"
     CDS             complement(1675..>2638)
                     /locus_tag="VGB68_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY0050484.1"
                     /translation="NGATLYEPAGLTPIFSGLAQIPVFPWKAAPTLGHIKGEARRADN
                     TPLDTAAVTIKNLDDNTTRAAATDGGGFYGGVDLTPGRYLVRAALGQDVLYSCVANVA
                     PGSVTTADLGAETVAPVTTVALAPAAPNGSNGWYTTDVTLSLAATDNCSGVERTEYST
                     DGGANWQPYAGNISISREGSTTISYRSIDRAGNIENTGSTTVNIDKTAPEFQLEATPT
                     RIFPPNGKPVTVKLTGTGSDSTSGLSQVSYAVTDEYGTPLSVPTRALSGNSASWIDNL
                     IVEARRNGDDSDGRLYRVTATVTDAAGNATSATVDIVVSHDRRP"
BASE COUNT          573 a          709 c          764 g          592 t
ORIGIN      
        1 aaccaaatgc acggacgaca aatttcccgc cggtgttttt ctcgaagccg gttacaattc
       61 ggcttttctc ggtcagaaat cctacaacgg cgtggcgatt ctctcgcctt cggaaatcgg
      121 cgacgtgcag atgaattttc ccgacgacga ccgggacgcg ccgaaacgcc ttattgcggc
      181 gacgattaac ggcgtgcgcg tcgtcaatac ttatattccg aacgggacgg agctttggac
      241 cgacaaattt acttttaaac tggattggct acagcggctg cggcgttttt tcgatgaaac
      301 ctgcgacaaa aattccgacg ttttattgtg cggcgatttt aacgtcgcgc ccgacgaacg
      361 cgacgtctgg aacgtcggtt tctgggaagg gaagctgcat ttttccaagc cggagcgcgc
      421 cgcgattcat cacgtcaaac agtggggctt tgtcgatgtt ttccgccagc tgaacggcga
      481 cgaaaagctg tattcgtggt ggaattaccg ggagggcgcg tttttcaaaa atcagggctt
      541 gcgcatcgac cacatctgga cttcgcgcag cctgtcggaa aaatgcacgg cttgctggat
      601 tgaccgaaaa ccgcgcggcg cggaaagacc gtcagaccac gcgccggtcg tggcggaatt
      661 tgagatttga gattaagagg cgaaagccgt cgcgcgaacg agggcgcatt tgatgagcaa
      721 ttctgcgcct ttgcccgcgc gccgtcttcc gcctttttga tttgcggcgg aacggcttga
      781 aatttctgag ccctggagca aaagttctat aaacttagtt tcataacttt atgaaagcct
      841 cgacaaaaaa ctgcgaagtt gaaattttcc cgaatgtcgc cgcgctcaat caagccgcgg
      901 cgcgctcgat tgtcgaaatc gcggcgaaag ctttgcaggc aaacgataaa ttttcaatcg
      961 cgctggctgg cggctcgacg cccgaagcgc tctataagat gctcgcttcc ggcgaattta
     1021 aaaacaggat tgattggacg aaaacgctcg tttttttcgg cgacgaacga tgcgtttcgc
     1081 ctgaaaatgc ggaaagcaat tatcgaatgg cgaatgcggc tctgctggcg cgcgttccgc
     1141 ttccgccgga aaatgttttt cgcctgcgcg gcgaaacgga gccggaaaaa gcggcgttcg
     1201 aatatgaaga aacgatcaaa aaaactctgg gcgaaaatgc gcgttttgat ttgattctgc
     1261 tcgggatggg cgacgacggg cacacggctt cgctgtttcc gcggacagag gctttgagcg
     1321 cagccggaaa gcttgtcgcg gcaaattacg tcgaaaaatt aaaggcgaac cggctgacgc
     1381 tgacgttccg cgccattaat tcggcgcgaa acgtcttgtt tctggtcgcc ggcgcaggga
     1441 aagccggaat gatgaaaaaa gttctctccg tcgaaacgat tgatttgcct tcgagcctaa
     1501 tcaagccgga aaacggaagc tgccgctggt ttttagaccc ggaagcggca gcactgattg
     1561 aaattttata aaagcgcatt cgcccggaag ctttgtaaaa cgctgattta aaagaattaa
     1621 tagagataag ttaatgctaa aatcaactta tctccattac tctaaaaaat ccggctacgg
     1681 tctccggtcg tgcgacacga cgatgtcgac cgtcgcgctc gtcgcgttgc cggcggcgtc
     1741 ggtgaccgtc gccgtgactc gatataagcg cccgtccgaa tcatctccgt tgcgtctcgc
     1801 ctcgacaatc agattatcta tccagcttgc cgaattgccg ctcaatgcac gggtcgggac
     1861 gctcagcggc gtaccgtatt cgtccgtcac cgcgtaactg acctgcgata atccggaagt
     1921 cgaatccgag ccggtgccgg ttaatttgac cgtgacgggt tttccgttcg gcggaaagat
     1981 gcgcgtcggc gtcgcttcga gctgaaattc cggcgcggtt ttgtcgatat tgacggtcgt
     2041 gctgccggta ttttcgatat tgcccgcgcg gtcgattgag cgatacgaaa tcgtcgtcga
     2101 gccttcccgg ctgatggaaa tatttcccgc gtaaggctgc cagtttgcgc cgccgtcagt
     2161 tgaatattcc gttcgctcga cgccggagca attgtcggtc gccgcgagac tgagcgtgac
     2221 gtcggtcgta taccagccgt tcgagccgtt cggcgcggcg ggcgcgagcg cgaccgtcgt
     2281 gaccggcgcg accgtttccg ctcccaaatc cgccgtcgtg accgaaccgg gcgcgacatt
     2341 ggcgacgcac gaataaagca cgtcctgacc gagcgccgct ctgaccagat agcgacccgg
     2401 agtcaaatcg acgccgccgt aaaatccgcc gccgtcggtt gccgccgcgc gcgtcgtgtt
     2461 gtcgtcgaga tttttgatgg tcacggcggc ggtgtcgagc ggcgtattgt cggcgcggcg
     2521 cgcttcgcct ttgatgtgcc cgagcgtcgg cgcggctttc cacggaaaaa cgggaatttg
     2581 cgccagcccg ctgaaaatcg gcgtcagtcc ggcaggctcg taaagggtcg cgccgttg
//
LOCUS       DASWNE010000740         3739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_164004, whole genome shotgun sequence.
ACCESSION   DASWNE010000740 DASWNE010000000
VERSION     DASWNE010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164004"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1174
                     /locus_tag="VGB68_14435"
     CDS             116..1174
                     /locus_tag="VGB68_14435"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY0050485.1"
                     /translation="MNNQLRNLKWWKTGWLFFALYTFYPLFFFPQYYFNGSHPAILVR
                     LFFQSYLWIILTPFMLWLGRRYSIVYPFRLKNIVILSLSPFLFAAAHVALFMLFWQVL
                     LAGAGLVPPVRLNIVFYMFTSATVFCAAILAFHQAFNYFRKFRDREFRLQEAELQLLK
                     SQLHPHFLFNSLNAISALIYISPRTAHETVSQLSDLLRASLKSGKAQEVSLEEELDFL
                     NKYVQIHRMLMGRRLDFVCNIEPEIMSAAVPNMILQPIVENSIQHGIAPLEQGGRIEL
                     SVSRQNRRLLLLQVTDNGKGLPEDKIVLNSNGIGLVNTKARLTHLYGDSCDFKITEPP
                     GGGVSVSLAIPYRDLNDE"
     gene            1176..1943
                     /locus_tag="VGB68_14440"
     CDS             1176..1943
                     /locus_tag="VGB68_14440"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HEY0050486.1"
                     /translation="MKIKTLIVDDVELAREHVKILLKDPEFEIVGECANGRETIRSIQ
                     ALKPDLVFLDVQMPEIGGFDVVEAIGAENMPLVIFVTAYDEFALRAFDVHALDYVLKP
                     IDEARFTRTVNHVKNQLRRGQNDDEAKLHSLLKQIGKERRYASRILIKSSRQTVLLPV
                     DEIDWVGAAGNYLEVHAGSKSYLLRERMNSFEQKLNPDTFARIHRSTIINLNRVKTMQ
                     VLYNDDHLVILHNGEKLRMSRGYFDKLASRLDAGQDN"
     gene            complement(2349..>3739)
                     /locus_tag="VGB68_14445"
     CDS             complement(2349..>3739)
                     /locus_tag="VGB68_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050487.1"
                     /translation="TRENHTTRSGHCQEKNNEPIDNTENKATNIRGDGSSLSNQKIDP
                     NSPDVLEGSAIIDNNDKNRPDAPKMPVTRISWKLRRCSPPLVITDVKFYHRRYPSPNT
                     WKEIKSENNDYTIDGNDVKIVATIVNLSGAQKSATVNFKDLTDNKDLLQGNVPASFAP
                     HEEKQVEYVWDTSGYAWKEAEPENYAVNSRRIQVRIPDDQKIEFVFVYPKPVVLIPGM
                     WSKPEKFEYLASYFKNQNVPWVIVVPPVYINRTAVSNAQIIDKAVREIQEKENAWHVD
                     LVAHSTGGLAARAYVDSLMPTQFDNRPTALHLVMIGTPNLGTPCASGGDNIITKNFNR
                     GAEAFGEISHENMLTFNETVTRRNGTRFMAVASKAISPTCRLDEPGDGVVPVISAIYK
                     SKTYAYTNSTYEDLGGDRDVFTHVRKWLALTPSHNFAPDNTAKLQDFGDERVPGTDEE
                     PAANRSARYFSR"
BASE COUNT          839 a          953 c          954 g          993 t
ORIGIN      
        1 tccgcgcagg gattccttgc cgcgcagttt ggaacaaacg gcgatattcc ggctgccgcg
       61 gctttccggc gctgaggatt tttatacttt cgccccggcg tattaaaata caaggatgaa
      121 caaccagctg cgaaacttaa aatggtggaa aaccggctgg ttgtttttcg cgctgtacac
      181 tttttatcct ttattctttt ttccgcagta ttactttaac ggttctcatc cggcaattct
      241 tgtcaggctt ttttttcaaa gctatttgtg gataatttta actcctttta tgttgtggct
      301 cgggcgacgc tattcaatcg tctatccgtt tcggttaaaa aatatagtca tcctgtctct
      361 gtcgccgttt ttatttgccg ccgcacacgt cgcccttttt atgcttttct ggcaggttct
      421 gctggcgggc gcgggcttgg tgccgccggt caggctgaac attgtttttt atatgtttac
      481 cagcgcgacc gttttctgcg ccgcgattct cgcctttcat caggcgttca attactttcg
      541 caaatttcgc gaccgcgaat ttcggctcca ggaagccgag ctgcagctgc ttaaatctca
      601 gcttcacccg cattttcttt ttaattcgct caatgccatc tcggctttaa tttatatttc
      661 gccgcgcacc gcgcacgaaa ccgtcagtca attgagcgat ttgctcagag cctcgctgaa
      721 aagcgggaaa gctcaggaag tctcgcttga agaagaattg gattttttga ataaatacgt
      781 gcagattcac cgaatgctga tgggaagacg gcttgatttc gtgtgcaata tcgaacctga
      841 aattatgagc gccgccgttc ccaatatgat tttgcagccg attgtcgaga actcgattca
      901 gcacggcatc gcgccgctcg aacagggagg acgaatagaa ttaagtgttt cgcggcaaaa
      961 ccggaggctg ctccttctgc aagtcaccga taacggcaaa ggattgcccg aagataaaat
     1021 cgtcttgaac agcaacggta tcgggctggt caacaccaaa gccagattga cgcatctgta
     1081 cggcgactcg tgcgatttca aaataacgga accgccgggc ggaggcgtca gcgtcagctt
     1141 ggcaatacca taccgggatt taaacgacga ataagatgaa aattaaaact ctgatagttg
     1201 acgatgtcga attggcgcgc gagcacgtca agatattgct caaagacccg gaatttgaaa
     1261 tcgtcggcga gtgcgccaac gggcgcgaga ctatccggtc aattcaagct ctcaaacccg
     1321 acctcgtttt tctcgacgtg caaatgccgg aaatcggcgg cttcgatgtg gtcgaggcaa
     1381 tcggcgcgga aaatatgccg ctcgttatat tcgtcaccgc ctacgacgaa ttcgcgcttc
     1441 gcgcctttga tgtccacgct ttggattacg ttttaaaacc gattgacgaa gccagattta
     1501 ccagaaccgt caatcacgtc aaaaaccagc ttcgacgcgg gcagaacgac gacgaggcga
     1561 agctgcattc gctgcttaaa cagataggaa aagagcggag atacgccagt cgaattctca
     1621 tcaaatcatc aaggcaaacc gttctgctgc ccgtcgacga gatagattgg gtcggcgcgg
     1681 cgggcaacta tctggaagtc cacgcgggaa gtaaatccta cctgctgcgc gagcgtatga
     1741 attcctttga acaaaaattg aaccccgaca ctttcgcgcg aattcatcgc tcgacaatca
     1801 tcaatctgaa tcgggtcaag acgatgcagg tgctttataa cgacgatcat ctggtgattt
     1861 tgcacaacgg cgagaaactc agaatgagcc gcggttactt cgataagctg gcttcacgcc
     1921 tcgacgccgg acaggataat taaagataac cgaaaacgag cccggcaaga tttgcggctt
     1981 caggcgttcc cgccgaattt ttgccgatag tttttgcttt gcacaaaaat aaagaaaggg
     2041 aaaattgtaa attctaaatt gtaaattcta aattgatgtc cggtatcttt tgtgaagtaa
     2101 tttagaatta ataatttaga atttacaatt agccatcgga atttttctcg attttcttta
     2161 tttttgtgca aagcagatag ttttaccttt ttcgttcacc aaggcagcgg aaacgttcgg
     2221 cgaatcgtaa agaacgagcg aaaaacgcga gccgcccgtg accggaattt cgatttccga
     2281 cgatgcgttt gccgccgtcc ggctgccggt cgcgaaattc ggttcgccat cgccgcgcgt
     2341 tttgaagctt accggctgaa atatcgcgcc gagcggtttg ccgccggctc ctcgtcggtt
     2401 cccggtacgc gttcgtcgcc gaaatcctgc aattttgcgg tattgtcggg cgcgaagttg
     2461 tggctcggcg tcagagccag ccatttgcga acgtgcgtga aaacgtctct atcgccgccc
     2521 aggtcttcat acgtcgagtt cgtataagca taagttttgc tcttgtaaat ggctgagatg
     2581 acaggcacga cgccgtcgcc cggctcgtcg agccggcagg tcggcgagat cgctttcgaa
     2641 gcgaccgcca taaacctcgt tccgttgcgg cgcgtgacgg tttcattaaa cgtcagcata
     2701 ttttcgtgcg agatttcgcc gaacgcttca gcgccgcggt taaaattttt ggtgatgata
     2761 ttgtccccgc ccgaggcgca cggcgttccc agattcggcg tgccgatcat caccagatgc
     2821 agcgccgtcg gtcggttgtc gaactgcgtc ggcataaggc tgtcgacata agcgcgcgcc
     2881 gccagaccgc cggtcgaatg cgcgaccaga tcgacgtgcc aggcgttttc tttttcctga
     2941 atctcgcgca ccgctttgtc gataatctgc gcgttcgaaa ccgccgttcg attaatgtaa
     3001 accggcggga cgacaatcac ccacggaacg ttctgatttt taaagtagct cgccagatac
     3061 tcgaactttt ccggcttcga ccacatcccg ggaatcagga cgaccggctt cggatagacg
     3121 aatacgaatt cgattttttg gtcgtccgga atgcgcacct gaatccggcg gctgttgacc
     3181 gcgtaatttt ccggctcggc ttctttccag gcgtagccgg aagtatccca gacgtattcg
     3241 acttgttttt cttcgtgcgg cgcgaaagaa gccggaacgt tgccttgcag caggtctttg
     3301 ttatccgtca agtctttgaa attaacggtc gccgactttt gcgcgcccga cagattgacg
     3361 atcgtcgcca cgatttttac gtcgttgccg tcaatcgtgt aatcgttgtt ctcgcttttg
     3421 atttccttcc aggtattcgg cgacggatat cgccggtgat aaaatttcac atcggtaatc
     3481 accagcggcg gcgaacagcg gcgcagcttc cacgaaattc tggtaaccgg cattttcggc
     3541 gcgtccggtc tgtttttatc gttattgtcg atgatcgcgc tgccttctaa aacgtcggga
     3601 gaattcgggt cgattttctg gttggagagc gagctgccgt cgcctcgtat attcgtcgct
     3661 ttgttctcgg tgttgtcgat cggctcgttg tttttctcct ggcagtgacc gctgcgggta
     3721 gtgtgatttt cgcgggtgt
//
LOCUS       DASWNE010000741         1624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_129077, whole genome shotgun sequence.
ACCESSION   DASWNE010000741 DASWNE010000000
VERSION     DASWNE010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129077"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..>1624
                     /locus_tag="VGB68_14450"
     CDS             20..>1624
                     /locus_tag="VGB68_14450"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0050488.1"
                     /translation="MSEDEARKIMQDADIIVNGAGNVTFNPPLESALRTNVVGSHNIL
                     QLARMMRRPTLVHVSTCFVAGKRSGTIWENEPVAGYFPRKNELVGTTFDVNKEIEDCA
                     RLSEQARQEATDAVQIAKFREQARERFIEEGRDPDDEDELKSAIFRERKMWIRERTTE
                     LGEARADYWGWTNIYTYSKSLAEQIIAQQDDIVKTLVRPSIVESANQYPFPGWNEGFT
                     TTAPLILIALRGQPIIPVNEKLVLDIIPVDMVAGAILGAAMNALIDDKPPLVFQACSG
                     DSNPSDMKRIVGLVGLYKREHFKEKETGNKLVNRAASMIEAIPVTHRTYELTSAPMLN
                     KLAKRANNLLDKAAPGWGGGRLGNIVSDLKKSVDGFERTTAETVAAFDLFKPFMIDNE
                     YLYRADNVRALMSLIKEKEKNLLLWFPEKLDWYDYWLKVHFPGLRKWVLPQLEEDLKI
                     QERRAYTYKDLLDLFDTSTKRFSTRVAMRIDRNGRKEQYTYDDVRELTLRAAGFFAAG
                     GIKHNDRVVLFSHNMPEWGLSYFGILK"
BASE COUNT          395 a          415 c          468 g          346 t
ORIGIN      
        1 cggcaatgaa tttctcggca tgagcgaaga cgaagccaga aaaatcatgc aggacgcgga
       61 cattatcgtc aacggcgcgg gcaacgtgac gtttaacccg ccgctcgaaa gcgctttgcg
      121 gacgaatgtc gtcggctcgc acaatatttt gcagctcgcg cggatgatgc ggcgaccgac
      181 gctcgtgcac gtttcgacgt gtttcgtcgc cggaaaacga agcggcacga tttgggaaaa
      241 cgaacccgtc gccggttatt ttccgcgaaa aaacgagctg gtcggcacga ctttcgacgt
      301 caacaaggaa atcgaagact gcgccagatt gagcgagcag gcgcggcagg aagcgaccga
      361 cgccgtgcaa atcgccaaat ttcgcgagca ggcaagagaa cgttttatcg aggaaggacg
      421 cgacccggac gacgaggacg aactgaaatc ggcgattttt cgcgagcgca aaatgtggat
      481 tcgcgagcgg acgaccgaat tgggcgaggc gcgcgccgat tattggggct ggacgaatat
      541 ctacacttat tcgaaatcgc tggcggagca aatcatcgcg cagcaggacg atatcgtcaa
      601 aactctggtg cgtccttcga ttgtcgaatc ggcaaatcaa tatccttttc cgggctggaa
      661 cgagggcttt acgacgaccg cgcctttgat tctcatcgcg ctgcgcgggc agccgattat
      721 cccggtcaac gaaaagctcg tgctcgacat aattccggtc gatatggtcg cgggcgctat
      781 cttgggcgcg gcgatgaacg ctttgattga cgacaagccg ccgctggttt ttcaggcgtg
      841 ttcgggcgat tcgaatccga gcgatatgaa gcgcatcgtc ggtctggtcg ggctttataa
      901 acgcgagcat ttcaaggaaa aagagacggg caataaactg gtcaatcgcg ccgcctcgat
      961 gattgaagcg atacccgtca cgcatcgaac ctacgaatta acctcggcgc cgatgctcaa
     1021 caaactggcg aaacgcgcca ataatctgct ggacaaagcc gcgccgggct ggggcggcgg
     1081 gcggctggga aatatcgtct cggatttgaa aaaatcggtt gacggctttg agcgcacgac
     1141 cgctgaaacc gtcgccgctt tcgacctttt caagccgttt atgattgaca acgaatatct
     1201 ctatcgcgcc gacaacgtgc gggctttgat gtcgctcatt aaggaaaagg aaaaaaatct
     1261 tctgctctgg tttcccgaaa aactggattg gtacgattac tggctgaaag tgcattttcc
     1321 gggcttgcgc aaatgggttc tgccgcagct cgaagaagat ttgaagattc aggagcgccg
     1381 cgcctacacg tataaggatt tgctcgattt gttcgacact tcgaccaaaa gattttccac
     1441 gcgcgtggcg atgcgcatcg accgcaacgg gcgcaaagag caatatactt acgatgacgt
     1501 gcgcgagctg acattgcgcg ccgccggatt tttcgccgcc ggcggcatca aacataacga
     1561 tcgcgtggtt ttgttttcgc ataatatgcc cgaatggggc ttgtcttatt tcgggatttt
     1621 aaaa
//
LOCUS       DASWNE010000742         6121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_201691, whole genome shotgun sequence.
ACCESSION   DASWNE010000742 DASWNE010000000
VERSION     DASWNE010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6121
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201691"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /gene="def"
                     /locus_tag="VGB68_14455"
     CDS             <1..422
                     /gene="def"
                     /locus_tag="VGB68_14455"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY0050489.1"
                     /translation="DEDLRRLVKDMFETMYAAEGVGLAAPQIGLNLRLFVMDCEGIKL
                     VAANPEIVSAIGEQSGQEGCLSVGKVPAVVIRASKVKLKAQNVKGEWFEREAEGYAAR
                     CFLHETDHCDGRLFIDHLPKMRREMVVKRFQKEKKWK"
     gene            complement(425..1225)
                     /locus_tag="VGB68_14460"
     CDS             complement(425..1225)
                     /locus_tag="VGB68_14460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEY0050490.1"
                     /translation="MYPYPNLISESLLITETIELLQSYGGRASAVDIVDIVMRISAPD
                     SDLAKMLVLDLVETDPRLQLIEETVEFIPLNHDSRKLLESDFVVFDLETTGAKCPPCR
                     VTEIGAYRIENGQIVGEFQTLVNPETSIPPFIVALTGITDSMVRNAPKFKEVVSDFLD
                     FIGDAVLVAHNAHFDIRFLNNEIGRIYDSYRVANPHLCTVQLSRKLLPQIENHRLHTV
                     ADYYSINIENRHRAGDDAYATAKIFVNLLNQLQNLGVRDLATAKKFRP"
     gene            1339..1680
                     /locus_tag="VGB68_14465"
     CDS             1339..1680
                     /locus_tag="VGB68_14465"
                     /inference="COORDINATES: protein motif:HMM:NF027572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0050491.1"
                     /translation="MREENIKIVEAYLNALKQMDLSLAPLADDIAFENPLAGKNTGAE
                     NFRAYLSGFLLAINDVHVHQHICEDDYVATHWEIDAVFGIIPILEKFRVRDGKIVEAF
                     GFFDPRPIVGS"
     gene            1778..2350
                     /gene="pyrE"
                     /locus_tag="VGB68_14470"
     CDS             1778..2350
                     /gene="pyrE"
                     /locus_tag="VGB68_14470"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEY0050492.1"
                     /translation="MQILNHFKETGALLEGHFVLSSGLHSPNYLQCALALQYPADALK
                     FGRAIAEHFTGENIETVASPAIGGLIIGHAVAQALNVRFIWTERENGSMTLRRGFSIR
                     ENERILVVEDVITTGGSTRECIEALESRGAKVAGAASIIDRSGGRADVGVPRIALATL
                     EVPSYKTEDCPLCARGEVAVKPGSRAQIKI"
     gene            2347..2814
                     /locus_tag="VGB68_14475"
     CDS             2347..2814
                     /locus_tag="VGB68_14475"
                     /inference="COORDINATES: protein motif:HMM:NF016178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEY0050493.1"
                     /translation="MKISPQIEKFWKEFCAANPQVKPDTFFQVWHFGIGQDDAEELLN
                     LVLEGKKTATASLPWEYEDKPEDAPVLGCYSVVTDFAGNPKCIVRTTEIRVLPFNEVD
                     AAFAFDEGEGDQSLDYWRAVHWDYFSRRCSDLGKEPDTEMLVNCERFELLYQR"
     gene            2811..3563
                     /gene="truA"
                     /locus_tag="VGB68_14480"
     CDS             2811..3563
                     /gene="truA"
                     /locus_tag="VGB68_14480"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEY0050494.1"
                     /translation="MNFKLLIQYDGTDFHGWQVQENQRTVQGELQRVLSLLENAEVHV
                     AGSGRTDAGVHAEGQVGSAKLAREFTPEKLRNAINGNLWRDLRILSVEKASDDFHARF
                     SARGKTYVYRVVNAPVMSPFWVRYAHHEARPLDVGRMNDTARLFLGEHDWTAFSSAQS
                     DSENKVRTITEFTVESFWDNRAKGSLIEFRITGEGFLRYMVRSIAGTLLEVGRGEKDF
                     DTIQTAIVSRDRNLAGATAPAHGLTLLKVYYD"
     gene            3569..3871
                     /locus_tag="VGB68_14485"
     CDS             3569..3871
                     /locus_tag="VGB68_14485"
                     /inference="COORDINATES: protein motif:HMM:NF017880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HEY0050495.1"
                     /translation="MLIYHIVLPEAWEKFKDLPFYEHESLESEGFIHCSYRNQLEEVL
                     RRYYKNEKKVVILHISPARLAADLIAERSTGGEVYPHIYGKINQTAIVNLEERILG"
     gene            3882..4676
                     /locus_tag="VGB68_14490"
     CDS             3882..4676
                     /locus_tag="VGB68_14490"
                     /inference="COORDINATES: protein motif:HMM:NF011405.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEY0050496.1"
                     /translation="MLEHFAGVVKPESEDAKLLAAIDWSRLPQHVAVIMDGNGRWARA
                     RGKPRIFGHRAGAESVRALVDTTARLGLKALTLYAFSTENWKRPQDEVSGLMQMLKRF
                     LKSELKTVHKNNIKFQAIGNINGLASDVQKQLAFAEEITAGNTGTILSVALNYGGRAE
                     ILEACRKAVRFLAENGDSIEDLREEHIERNLYTHNLPEVDLLVRTSGEMRISNFLLWQ
                     LAYSEIYVTPTLFPDFRRAQFLEAILDFQKRDRRFGGIAASKENRG"
     gene            4729..5139
                     /locus_tag="VGB68_14495"
     CDS             4729..5139
                     /locus_tag="VGB68_14495"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEY0050497.1"
                     /translation="MKVFLSYAHKDIDLAKKIEAVLEGEGLQVWNAETEILPGDNFAE
                     KISDALKASDAMVVLLTPESLNSKWIQWEIEYALSNKSYNRRVIPVLVGSEESVSLES
                     IPWILRKLQMIRLSKPEYAEREINQITEALKLAA"
     gene            5153..5566
                     /locus_tag="VGB68_14500"
     CDS             5153..5566
                     /locus_tag="VGB68_14500"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEY0050498.1"
                     /translation="MPPGEVFLSHSSLDKDFVDSIAETLGNHGIPVWYSRTNILGAHQ
                     WQDEIGQALRRCDWFIVILSPKSVQSMWVKRELAFALNDNKYNNKIIPILYQPCDFNT
                     LSWTLSLFEIIDFSGDLNTGFSELLRTWGIGYRNQ"
     gene            5567..>6121
                     /locus_tag="VGB68_14505"
     CDS             5567..>6121
                     /locus_tag="VGB68_14505"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEY0050499.1"
                     /translation="MKTRLLTALVALPVLILSVILPYFLPNRAEADWLFLLIAALGLG
                     AGLFEFFSMTKKLELKADAAIAYLGASAFFVAFLFDAPAKAPDLLILTLAFFLISVLV
                     TQMFRFQADFSKMLTGVGVTVLGVLYIAFLGGFLISTRVGFDATLVPHLSTKLLAFFF
                     LVAFGADTGAYFAGKNLGKHKLIPK"
BASE COUNT         1549 a         1432 c         1633 g         1507 t
ORIGIN      
        1 tcgacgaaga tttgcgccgt ctggtcaagg atatgttcga gacgatgtac gcggcggaag
       61 gcgtcggctt ggcagcgccg caaatcgggc ttaatttgcg attgttcgtg atggattgcg
      121 agggcatcaa gctcgtcgcc gccaatcccg aaatcgtctc ggcgatcggc gagcagagcg
      181 ggcaggaagg ctgcctgtcg gtcggcaagg ttccggcggt cgtaattcgc gcgtcaaaag
      241 taaaattaaa agcgcagaac gtcaaaggcg aatggttcga gcgcgaagcc gaaggctacg
      301 ccgcgcgctg ttttctgcac gaaaccgacc attgcgacgg caggctcttt atcgaccacc
      361 tgccgaaaat gcggcgcgag atggtcgtga aaagatttca gaaagagaaa aagtggaagt
      421 aaagttaagg tctgaatttc ttcgccgtcg ccaaatctct gacgccaagg ttttgcaatt
      481 gattcagcaa attaacgaaa attttcgccg tcgcgtaagc gtcgtcgccg gcgcggtgac
      541 ggttttcgat gttgattgag taataatcgg cgacggtgtg caggcggtga ttttcaatct
      601 gcggaagcag cttgcgcgaa agctgaaccg tgcacagatg cggattcgcc acgcgatagc
      661 tgtcgtaaat gcgcccgatt tcattgttca aaaagcgaat gtcaaaatgc gcgttgtgcg
      721 cgaccaggac ggcgtcgccg ataaaatcca gaaaatccga aacgacttcc ttaaacttcg
      781 gcgcgtttct caccatcgaa tcggtaattc ccgtcaaggc gacgataaac ggcggaatcg
      841 acgtctccgg attgaccagc gtttgaaatt cgccgacgat ttgcccgttt tcgatgcgat
      901 acgcgccgat ttccgtcacg cggcagggcg ggcatttcgc gccggtcgtc tccaaatcga
      961 aaacgacaaa atcgctttcg agcagcttgc gcgaatcgtg attcagcgga atgaactcga
     1021 ccgtttcctc gattaattgc aggcgcgggt cggtttcgac caaatccagc accagcattt
     1081 tcgccagatc ggaatcgggc gcggaaatgc gcataacaat atcgacaata tcgactgccg
     1141 acgcgcgacc gccgtaagat tgcaaaagtt caatcgtttc ggtgattagc aaggactcgg
     1201 aaattaagtt aggatacgga tacattgctg atttattcta aacacgaatt tctttgcgct
     1261 acaagcgcaa ccgtaaaatc ttccgcgaca aaagcttatt ttgtgacttt ttattaaatt
     1321 ttcgaggaga tttttgaaat gagagaagaa aatatcaaaa tcgtcgaagc ttatctgaat
     1381 gcgttaaaac aaatggattt atcgctcgcg ccgcttgccg acgacatcgc gtttgaaaac
     1441 ccgctggcgg gaaaaaacac gggcgcggaa aattttcggg cgtatttgtc cggttttctg
     1501 ctcgcgataa atgatgttca cgttcatcag cacatttgcg aagacgatta tgtcgcgacg
     1561 cactgggaga ttgacgcagt ttttggcata attccgattt tggaaaaatt ccgcgtgcgc
     1621 gacggaaaaa tcgtcgaagc attcggcttt ttcgacccca gaccaatcgt tggaagttga
     1681 gagttgactg aaagttgacc gagttgagcg agttatcaga taggctcgac taactcagcc
     1741 aacttcagcc aacttcagtc aacttcagtc aacttttatg cagattttaa atcatttcaa
     1801 agaaaccggc gcgcttctgg aaggtcattt tgttttgtcg agcggcttgc acagcccgaa
     1861 ttatctgcaa tgcgctctgg ctttgcagta tccggcggac gcgttaaaat tcgggcgggc
     1921 gattgccgaa cattttaccg gcgaaaatat cgaaacggtc gcctcgcccg ccatcggcgg
     1981 tttgataatc ggtcacgcgg tcgctcaggc tttaaacgtg cgttttatct ggaccgagcg
     2041 cgaaaacggc tcgatgacgc tgcggcgcgg cttttccatc agggaaaacg agcggatttt
     2101 agtcgtcgaa gacgtgataa cgaccggcgg ctcgacgcgc gaatgcatcg aagcgctgga
     2161 aagtcgcggc gcgaaagttg cgggcgcggc ttcgataatc gaccgctcag gcgggcgcgc
     2221 cgacgtcggc gttccgcgaa tcgctttagc gactttagaa gttccgagct acaaaaccga
     2281 ggattgtccg ctgtgcgcgc gcggcgaggt cgccgtcaag ccgggaagcc gcgcgcaaat
     2341 taaaatatga aaatttcacc gcaaatcgaa aaattctgga aagaattctg cgcggcaaat
     2401 ccgcaggtaa agcccgacac gttttttcag gtctggcatt tcggaattgg acaggatgac
     2461 gccgaagaat tattgaatct ggttttggaa ggcaaaaaaa cggcgaccgc atccttgccg
     2521 tgggaatatg aagacaagcc ggaggacgcg ccggttctcg gctgttacag cgtcgtgacg
     2581 gattttgcgg gcaatccgaa atgcattgtg cggacgacgg aaattcgcgt tttgcctttt
     2641 aacgaagttg acgcggcgtt tgcgtttgac gaaggcgaag gcgaccagag cctcgattac
     2701 tggcgagcgg ttcactggga ttatttttcg cgaagatgct ctgatttagg caaagagccg
     2761 gacacggaaa tgctcgtcaa ttgcgaaagg tttgaattgc tctatcaaag atgaatttca
     2821 agcttttgat tcaatacgac ggcacggatt ttcacggctg gcaggtgcag gaaaatcagc
     2881 gcacggtgca gggcgagctg cagcgggttt tgtcgctgct cgaaaacgcg gaagttcacg
     2941 tcgccggttc gggcaggacc gacgcgggcg tccacgccga aggacaagtc ggcagcgcga
     3001 agctcgccag agagtttacg ccggaaaaac tgcgcaacgc gattaacgga aatctctggc
     3061 gcgacctgcg gattttgagc gtcgaaaaag cgtcggacga ttttcacgcg cgtttttccg
     3121 ccagaggcaa aacttacgtt taccgggtcg tcaacgcgcc cgtaatgtcg ccgttctggg
     3181 tgcgctacgc gcatcacgaa gcccgcccgc tcgatgtcgg cagaatgaac gacacggcgc
     3241 gtttattttt gggcgagcac gactggacgg cgttttcctc ggcgcaatcc gacagcgaaa
     3301 acaaggtgcg cacgattacg gaatttacgg tcgaatcttt ttgggacaat cgcgccaaag
     3361 gctcgcttat agaatttcgc atcacgggcg aaggtttttt gcgttatatg gtgcgctcga
     3421 ttgccggaac tcttttggaa gtcgggcgcg gcgaaaaaga ttttgataca attcaaacgg
     3481 cgattgtcag ccgcgaccga aatctggcgg gcgcgaccgc tccggcgcac ggcttgacgc
     3541 tgctgaaagt ttattacgat taattaaaat gctgatttat catattgttt tgcccgaagc
     3601 ctgggaaaaa ttcaaagacc tgccgtttta cgaacacgaa agcctcgaaa gcgaaggctt
     3661 tattcattgc agctatcgca accagctgga agaggttttg cggcgctatt ataaaaatga
     3721 aaaaaaagtc gtgattctgc acatcagtcc ggcgcgtctg gcggcggatt taatcgccga
     3781 gcgctcgacc ggcggcgagg tttacccgca catctacggg aaaataaatc aaacggcgat
     3841 tgtcaatctc gaagaaagaa ttttaggata aaccagaagt catgcttgaa cacttcgccg
     3901 gagtcgtcaa acccgaatcg gaagatgcaa aattgctggc ggcaattgac tggtcgcgcc
     3961 tgccgcagca cgtcgccgtc attatggacg gcaacgggcg ctgggcgcgg gctagaggaa
     4021 agcctcgaat tttcgggcat cgcgccggag ccgaatcggt tcgcgcgctg gtcgatacga
     4081 ccgcgcggct cggcttgaaa gccttgacgc tttacgcctt ttcgaccgaa aactggaaac
     4141 gcccgcagga cgaagtttcc ggtttgatgc agatgctcaa acgctttctg aaaagcgagc
     4201 tgaaaaccgt ccataaaaac aatattaaat ttcaggcgat cggaaacatc aacggtttag
     4261 cttcggacgt gcaaaaacaa ctcgcctttg ccgaggaaat taccgccggc aatacgggaa
     4321 cgattttgag cgtcgccctt aactacggcg ggcgcgccga aattctcgaa gcctgccgca
     4381 aagccgttcg ttttctggcg gaaaacggag attcaatcga agatttgcgc gaagaacata
     4441 tcgagcgcaa tctttacacg cacaatctgc cggaagtcga tttgctggtc agaacgagcg
     4501 gcgagatgcg gatttccaat tttcttttgt ggcagctggc ttacagcgaa atctacgtca
     4561 cgccgacgct gtttcccgat tttcgccgcg cgcagtttct ggaagcgatt ctggattttc
     4621 aaaaacgcga ccggcgcttc ggcggtattg ccgcatcgaa ggaaaataga ggttgatttt
     4681 tcttgtttta actctaaaat aaaacaagaa ttcggatgtg aggtaaaaat gaaggttttt
     4741 ttaagttacg cgcataaaga cattgatttg gcaaagaaaa tagaggcggt gctcgaaggt
     4801 gaaggcttgc aggtatggaa tgctgaaacg gaaatattac ccggagacaa ctttgccgaa
     4861 aaaatttccg acgcgttaaa agcgtctgat gcaatggttg ttttgcttac accggaatcg
     4921 ttaaattcaa aatggattca atgggaaatc gagtatgctc tgagtaataa atcttacaac
     4981 cggcgcgtta tacctgtttt agtcggttct gaagagagtg tttctctgga aagtattccc
     5041 tggattttaa gaaaacttca aatgattcgg ttatcaaaac ccgaatatgc agaaagggaa
     5101 ataaatcaaa ttaccgaagc tctgaaatta gccgcctaac taaaaaaaat ttatgccgcc
     5161 tggagaagtc tttctttctc attccagttt agacaaagac tttgtcgatt caattgctga
     5221 aactttagga aatcacggaa ttccggtttg gtatagccgg acaaatattc tcggcgcgca
     5281 tcaatggcaa gacgagatag gtcaggcatt acgacgctgc gattggttta tcgttattct
     5341 ctctccaaaa tcagttcaat caatgtgggt gaaaagagag ctggcatttg cattaaatga
     5401 taacaaatac aacaacaaaa taattcctat tctctatcag ccttgtgact tcaacactct
     5461 ttcctggaca ttatctttat ttgaaataat tgatttctca ggcgatttga ataccggatt
     5521 tagcgagtta ttaagaactt ggggaatagg ttacagaaat caataaatga aaactcgact
     5581 tttaaccgcg ctggttgcgc ttccggtttt aattttatca gtcattctgc cgtatttttt
     5641 gccgaatcgc gccgaggcgg attggctttt tctgttgatt gccgcgctcg gtctgggcgc
     5701 ggggcttttc gagttttttt cgatgactaa aaagctggag ctcaaagccg acgccgcgat
     5761 tgcttatctc ggcgcttcgg ctttttttgt cgcgtttctt ttcgacgcgc cggcaaaagc
     5821 gccggattta ttaattctca cgctcgcgtt tttcctgatt tcggttctcg tcacgcagat
     5881 gtttcgcttt caggcggatt tctcgaaaat gctgacgggc gtcggcgtga cggttctggg
     5941 cgttttgtac atcgcttttc tgggcggatt tctgatttcg acgcgcgtcg gatttgacgc
     6001 cacgctcgtg ccgcatcttt cgacaaagct tctcgcattc ttttttctgg tcgctttcgg
     6061 cgctgacacg ggcgcgtatt ttgccggaaa aaatctcggc aaacataaat tgattccgaa
     6121 g
//
LOCUS       DASWNE010000743         1598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_376162, whole genome shotgun sequence.
ACCESSION   DASWNE010000743 DASWNE010000000
VERSION     DASWNE010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376162"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="VGB68_14510"
     CDS             <1..833
                     /locus_tag="VGB68_14510"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEY0050500.1"
                     /translation="KLNRTALVTGKKSLILPCLGRSEKDFQNGAEQFVSTENTMLQVQ
                     QSKGIFEPLSEHLRSEPWIIAQLAKAVLGEKTTVEWDALVSDYDRIREAISRVVAGCE
                     NYNEKIRKPGGFYMPNQPREGVFPNKVGKALFKTSELRRIELKDGELLMTTIRAHDQF
                     NTTVYEHNDRYRGIRGSRRVIFMNEADIEARNLRNGQVVDLTSYFTDGQRRAEKFIVV
                     SYPIPRKCAATYFPEANVLVPLDSTAEKSNCPTSKLVIITVAPRLENGEKIFSGEFNR
                     "
     gene            complement(856..1218)
                     /locus_tag="VGB68_14515"
     CDS             complement(856..1218)
                     /locus_tag="VGB68_14515"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HEY0050501.1"
                     /translation="MKLINKLFAAAIMIFAFVNLGCGSKLTAESTAQITRVAIDRDTK
                     TSRRKTSSNKKRKTTTSVETEIDYRYTVGGKTYEGYSEKDGDVQRDFPSGAQVVVCYN
                     PSNPEESDVFAGGYKCGG"
     gene            1347..>1598
                     /locus_tag="VGB68_14520"
     CDS             1347..>1598
                     /locus_tag="VGB68_14520"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEY0050502.1"
                     /translation="MTTNFSGYVLAGGKSSRMKTDKAFLKLRGETFLERAVRALSRAC
                     GERVKIVINENQKAKFAKSFPSFDFVFDVFSERGALGGIQ"
BASE COUNT          393 a          409 c          410 g          386 t
ORIGIN      
        1 cgaaacttaa ccgaaccgct ctggtgacgg gcaaaaagtc cttgattctg ccttgtctcg
       61 gacgctcgga aaaagatttt cagaacggcg cggagcaatt cgtctcgacc gagaacacga
      121 tgcttcaggt gcagcagtcg aaaggaattt tcgagcctct gtcggagcat ctgcgcagcg
      181 agccgtggat tatcgcgcaa ttggcgaaag ctgttctggg cgaaaaaacg acggtcgaat
      241 gggacgcgct tgtttccgat tacgaccgga ttcgcgaagc catctcgcgc gtcgtcgccg
      301 gctgcgaaaa ttataatgaa aaaatcagaa agcccggcgg tttttatatg ccgaaccagc
      361 cgcgcgaagg cgtgtttccg aacaaagtcg gaaaggctct ttttaaaacc agcgagcttc
      421 gccgcatcga attaaaagac ggcgaattgt taatgacgac gatacgcgct cacgaccagt
      481 tcaacacgac cgtctacgag cacaacgacc gttatcgcgg catccgcggc tcgcggcgcg
      541 tgatttttat gaacgaagcc gacatcgaag cgcgcaatct ccgaaacggt caggtcgtcg
      601 atttaaccag ttatttcacc gacgggcagc gccgggcgga aaaattcatc gtcgtttcgt
      661 atccgattcc acgaaaatgc gcggcgacgt attttcccga agcgaacgtt ctcgttccgc
      721 tcgacagcac ggcggaaaaa agcaattgcc cgacttcaaa actggttatt ataacggtcg
      781 cgccgcgcct ggaaaacggg gaaaaaatct tttcgggcga atttaacaga taaattaatc
      841 tttctattta tccgtctagc cgccgcattt atagccgccg gcaaaaacgt ccgattcttc
      901 cgggttcgat ggattataac aaaccacgac ctgcgcgccc gacggaaaat cgcgctgcac
      961 gtcgccgtct ttttccgagt agccttcgta ggttttgccg ccgaccgtgt accggtaatc
     1021 gatttcggtc tcgacgctcg tcgtggtttt tcgctttttg tttgagctgg tttttcgacg
     1081 gctggttttg gtatcgcggt cgatggcgac gcgagtgatt tgcgcggtcg attcggcagt
     1141 caatttcgag ccgcatccga gattgacgaa ggcgaaaatc attatagccg ccgcaaacaa
     1201 tttgtttatt aatttcattt tcataattcg gcagaccgca ttttacaaca tctgccgtca
     1261 cgttttccaa atctcgcgcg gacttttttc agctgtgaaa agatgtttgc aatagtcggc
     1321 gctgaattaa ataatcggaa gcgacaatga cgacaaattt ttcaggttac gtgctggcgg
     1381 gcggaaaatc ttcgcggatg aaaacggata aagccttttt aaaactgcgc ggcgagacct
     1441 ttctcgaacg cgccgtccgc gcgctttcca gagcttgcgg cgagcgcgtc aaaatcgtta
     1501 tcaacgaaaa tcaaaaagcg aaatttgcaa aaagcttccc gtcgttcgat ttcgttttcg
     1561 acgtcttttc agaacgcggc gcgctcggcg gaatccag
//
LOCUS       DASWNE010000744         5029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_25325, whole genome shotgun sequence.
ACCESSION   DASWNE010000744 DASWNE010000000
VERSION     DASWNE010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25325"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            691..1629
                     /locus_tag="VGB68_14525"
     CDS             691..1629
                     /locus_tag="VGB68_14525"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEY0050503.1"
                     /translation="MKFPFPLPADKEFDAVGFGTNAVDFLIVAPEYPQFNSKIELVDY
                     VQLAGGEIASTMAGLCRLGLKTAYVGRFGDDEAGNFGLQTLIAEGVDTSRAEQIEGAR
                     TQVGFILIDEQNGERTVMWKRDEKLSYTPAEAPIEIVKNTRVFHATPHDALACARMAR
                     AAREAGAIVSIDIDNLFEGIEALLPLIDIFISSEEFPEKLIGAKDRRESLREIKSRYG
                     YKIAGMTLGEKGSLLLCEDEFIETGGFAVPNGCKDTTGAGDAFRVGLLYGLLTGESVE
                     TAARMANAVAALKCRELGARTALPKIEELNWLLDSV"
     gene            complement(1785..2171)
                     /gene="crcB"
                     /locus_tag="VGB68_14530"
     CDS             complement(1785..2171)
                     /gene="crcB"
                     /locus_tag="VGB68_14530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEY0050504.1"
                     /translation="MQTEVLTRILSVAIGGAFGAAARYGLNLLFAKTLAPFPFATFFI
                     NVTGSFLIGFLVILFADKFTVGENLRFAVIVGFLGAYTTFSTFEMEIFELIRERAFVT
                     AFLYLFSSVLIGFIGVSGGVWLAKKI"
     gene            complement(2408..2962)
                     /locus_tag="VGB68_14535"
     CDS             complement(2408..2962)
                     /locus_tag="VGB68_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050505.1"
                     /translation="MFHALILIVFLIVGLISADDAFAQTPDAQQKTQEIAASLDKTKY
                     KHKVKKDFEFELYINIKNEAVVRNAAEYAGVYESSDASHRVELRAVGGKIEGSGYDTN
                     FENSARVNFTLKDARIEGALLTANRIYADGTTKRLEAVFVNRTIVQGANPNKIESRET
                     KFGLGFVEIFNMNSQNRVFCEYKP"
     gene            complement(3217..3909)
                     /locus_tag="VGB68_14540"
     CDS             complement(3217..3909)
                     /locus_tag="VGB68_14540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEY0050506.1"
                     /translation="MEISVIIPTFNEELSITKTLDALSRLINVDEVIIVDGGSADKTI
                     EIIEGYDRLKKIKLVKIDEPNRGRQLHEGTKHASGDIFWFIHADTRPVQGAGRQIKAN
                     MRYREVAGGNFEITFGGANRWARFLTWLYPYLRSIGLVYGDSAIFVRREIYEKIGGFR
                     FLPIFEDLDLYKRLQKRGRFVHINLAVTTSSRRFENRSFLLTFLRWSFFQGLYWVGFP
                     PRILAKFYRPIR"
     gene            complement(4017..>5029)
                     /gene="carA"
                     /locus_tag="VGB68_14545"
     CDS             complement(4017..>5029)
                     /gene="carA"
                     /locus_tag="VGB68_14545"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEY0050507.1"
                     /translation="FGEMVFNTSMTGYQEILTDPSYAGQIVCMTYPLIGNYGVNEEDT
                     ESRRPWVEGFVVREASRIASNWRSQETLDSYLKRYKIVGIEHLDTRALVRHIRDKGAM
                     RAVISTTDADEASLLEKVKNSPSMKNRELASCVTIDETYDFPAAGEEKFHVVCYDFGV
                     KTNSLREFSKYGCRLTIVPAETPAGEVLEMKPDGIFLSNGPGDPASMQKVVEEIKKLT
                     EEGVPVFGICLGHQILGQVFGGETYKLKFGHRGGNQPIKDLSTGKVEITSHNHGFAVD
                     AASLPAEVEVTHVNLNDNTVAGLKHKNLPVFSVQYHPESAPGPHDSEYLFRRFIELME
                     KN"
BASE COUNT         1302 a         1193 c         1207 g         1327 t
ORIGIN      
        1 ttttttcgcg cccgttgacg ctgataatca tttctttcga catatttttt cctaaaatta
       61 tgattaggtg tcagtggatt atttttattt gagtcggcag tcaattagat ttctattgac
      121 tgccgaacgc tcgcgtgccg ctgactactt tttcaggctt ggagattcgt tttgcagaaa
      181 aatttcggat tggagataca gttttactta ccgctcgttt tagaaagcag atttcgcgtt
      241 ttggaactta gattttttta gtttccggca aaatgtggac tttaccgaaa gaatttgagt
      301 tttcacgcag gaatccgtgt ttttacaaaa gcgacttttc gcttttccat aacggggagc
      361 aaatgattat tttagatttg cgattttaga tttgcgattt gcggttggaa agagggtaag
      421 tttttcttaa caaattacgg cgcgagccgt tccttatcca atcgaaaatc gacaatcgaa
      481 aacctaaaat ttctcaatgc tcgacccgaa gatattcttt taacaaagct tatttgctgt
      541 tcaatccttt taagtttgct gtcatcaatc aaaaacgttt caaacgaaac tccaggcaaa
      601 cttcgctaag tataaatgac tttttttgtt ttgtcaccaa atatcgcgct tactgtcaac
      661 aaaagccttt ttgttttata cttttgcaga atgaaatttc ctttcccgtt acccgcagac
      721 aaagaatttg acgccgtcgg attcggcacc aacgcggttg attttttgat tgtcgcgccg
      781 gaatatccgc agtttaattc caaaatcgaa ctggtcgatt acgtccagct ggcgggcggc
      841 gaaatcgctt cgacgatggc tggactttgt cgtttaggct taaaaaccgc gtacgtcggt
      901 cgtttcggcg acgacgaagc gggcaatttc ggcttgcaga cgttaatcgc cgaaggcgtc
      961 gatacgagcc gcgccgaaca aatcgaaggc gcgcgcacgc aggtcggttt tatcttaatc
     1021 gacgaacaaa acggcgagcg cacggtgatg tggaaacgcg atgaaaaact ttcctacacg
     1081 ccggcggaag cgccaatcga gattgtgaaa aacaccagag tttttcacgc gacgccgcac
     1141 gacgcgctcg cctgcgcgcg aatggcgcgg gcggcgcgag aggcgggcgc gattgtctcg
     1201 attgacatcg acaatctttt tgaaggcatc gaggcgctgc tgccgctgat tgatattttt
     1261 atttcatccg aggaatttcc cgaaaagctt atcggcgcta aagacagacg cgaatctctg
     1321 cgcgagataa aatcgcgcta cggctataaa atcgccggaa tgactctggg cgagaaaggc
     1381 tctctgcttt tgtgcgaaga cgaatttatc gaaaccggcg gattcgccgt gccgaacggc
     1441 tgcaaagaca cgaccggcgc gggcgacgcc tttcgcgtcg ggctgcttta cggacttttg
     1501 acgggcgaga gcgtagaaac ggcggcgcga atggcaaacg ccgtcgccgc cctaaaatgc
     1561 cgcgagctcg gcgcgcggac ggctctgccg aaaatcgaag aattgaattg gcttttggat
     1621 tcagtctaga ttcgggattc agtctagatt caagattcag gattcaggat tcaggattgg
     1681 tctttggata acggtaaatc ctaaaccttg aatcttgaat cctgaatcct gaatcttaaa
     1741 tcttaaatcc tgaatcctga atcctgaatc ctgaatcctg aatcctaaat cttcttcgcc
     1801 agccagacgc cgcccgacac gccgataaaa ccaatcagaa cgctcgaaaa aagatataga
     1861 aaagccgtga cgaatgcgcg ttcgcgaatt aattcgaaaa tctccatttc aaaagtcgaa
     1921 aaggtcgtgt atgcgcctaa aaatccgacg atgacggcaa atcgcagatt ttcgccgacc
     1981 gtaaatttat cggcgaaaag aatcactaga aagccgatca gaaacgagcc cgtgacgttg
     2041 ataaaaaacg tcgcgaaagg aaacggcgcg agagttttgg caaacagcag attcaagccg
     2101 tagcgagccg ccgcgccgaa tgcgccgccg attgccaccg ataaaatccg cgttaaaact
     2161 tccgtctgca aaacctcgtt ttattaaaat cgatactaag aaaatttcaa cacaaagccg
     2221 cagggaacac aaagcggatt cagccgcgaa aggacacgaa aaacacgaac aaaaggagat
     2281 tagccgcaaa taatacgaac ggcacgaatg aaaggaagga gaaatttcgt gttattcgta
     2341 ttattcgcgg ctaattttct taattcttat ttcgcgcctt tccgccttct ttcgcggcta
     2401 aagcttctta tggtttatat tcgcagaaaa ctctgttttg cgaattcata ttgaaaattt
     2461 cgacaaatcc cagaccgaat tttgtctcgc ggctctcgat tttattcggg ttcgcgccct
     2521 gaacgatagt gcgatttacg aaaacggctt cgagtctctt cgtcgttccg tcggcgtaaa
     2581 ttctgtttgc cgtcagcaat gcgccttcga tgcgcgcatc tttcaaagta aaatttaccc
     2641 gcgccgaatt ttcaaaattg gtgtcgtaac cgcttccttc gattttgccg ccgacggcgc
     2701 gcagctcgac gcgatgactg gcatcggaag attcgtaaac gcccgcgtat tcggcggcgt
     2761 ttctgacgac cgcttcgttt ttaatgttta tgtagagttc aaactcaaaa tcctttttta
     2821 ctttgtgttt atatttcgtc ttgtcgagcg aagcggcgat ttcctgcgtt ttttgctgcg
     2881 cgtcgggcgt ttgagcaaaa gcgtcgtccg ccgaaatcaa gccgacgatt aaaaatacga
     2941 ttagaattaa agcgtgaaac ataaaaattt ctccttttaa aatgggttct aatcatataa
     3001 acaccggaag attccgtttt gtgacaggaa aattcgggat tcgggattta agattcagga
     3061 ttcaggattc aggattcagg attcaggatt caggattcaa gattcaggat ttaaaattta
     3121 aaattcggcg tttgagatgc gccgtcagca aacgaccaat cctgaatctt gaatcttgaa
     3181 tcttgaatcc tgaatcttaa atcttgaatc ttaaattcat ctaatcggtc gataaaattt
     3241 cgccaggatg cgcggcggaa agccgaccca gtagagccct tgaaaaaacg accagcgcag
     3301 gaaagtcagc agaaacgagc gattttcgaa acgccgcgaa gacgtcgtca ccgccagatt
     3361 gatatgcacg aaacgcccgc gtttttgcag gcgcttgtat aaatccaaat cttcaaaaat
     3421 aggcagaaag cgaaaaccgc cgattttttc gtaaatttcg cggcgcacga aaatcgccga
     3481 atcgccgtaa accagaccga ttgagcgcag gtatggataa agccacgtca aaaatcgcgc
     3541 ccagcgattt gcgccgccga aggttatctc gaaatttccg ccggcgacct ctcggtaacg
     3601 catattggct ttaatctgtc tgcccgcgcc ctgtacgggg cgcgtgtcgg cgtgaataaa
     3661 ccagaaaatg tcgccgctcg cgtgtttcgt gccttcgtga agctgccgcc cgcgattcgg
     3721 ctcgtcgatt tttaccagtt tgattttttt caaacggtcg tagccttcga taatttcaat
     3781 ggttttatcc gcgcttccgc cgtcgacgat gataacttcg tccacgttta tcaaacgcga
     3841 caaggcatcg agcgtctttg taatcgaaag ctcttcgtta aaagtcggaa tgatgacgga
     3901 gatttccata acgcgaacag tttaaagtga aaaggtgaaa aagtgaaaga gtgaaaaagt
     3961 aagcccggcg gatttcgaca aaactttttc gccttttcgc tttttcgctt tttcgcttaa
     4021 tttttttcca ttaattcgat aaagcgtctg aacaaatact cggaatcgtg cggaccgggc
     4081 gcggattcgg gatgatactg gacgctgaaa accggcagat ttttatgttt caatccggcg
     4141 accgtgttgt cgttcagatt gacgtgcgtg acttcgacct cggcgggcag gctcgccgca
     4201 tcgacggcaa aaccgtgatt gtgcgacgtg atttcgactt ttccggtcga taaatcctta
     4261 atcggctggt tgccgccgcg atgcccgaat tttaatttat aagtttcacc gccgaaaacc
     4321 tgtcctaaaa tctggtgacc gagacagatg ccgaacacgg gaacgccttc ttcggtgagt
     4381 tttttgattt cctcaacgac cttttgcatc gaagccgggt cgccgggacc gttcgagaga
     4441 aaaattccgt cgggcttcat ttccagaact tcgccggcgg gcgtttccgc cggaacaatc
     4501 gtcaaacggc agccgtattt ggaaaactcg cgcagagaat ttgttttgac gccgaaatcg
     4561 taacagacga cgtgaaactt ttcttcgccg gcggcgggaa aatcgtatgt ttcgtcaatc
     4621 gtcacgcagc tggcaagctc gcggtttttc atcgacggcg aattttttac tttttccaaa
     4681 aggctcgctt cgtccgcgtc ggttgtcgaa ataacggcgc gcatcgcgcc tttgtcgcgg
     4741 atgtggcgaa cgagcgcgcg cgtgtcgagg tgctcgatgc cgacgatttt ataacgtttg
     4801 agatacgaat ccagcgtttc ctgcgagcgc caattggaag cgatgcggct cgcttcgcga
     4861 acgacgaagc cttcgaccca gggacggcgc gattcggtat cttcctcgtt gacgccgtag
     4921 tttccaatca gcggatacgt catacagacg atttgcccgg cataactcgg gtcggtcaga
     4981 atttcctgat aacccgtcat cgaggtatta aaaaccatct cgccgaaag
//
LOCUS       DASWNE010000745         4095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_556113, whole genome shotgun sequence.
ACCESSION   DASWNE010000745 DASWNE010000000
VERSION     DASWNE010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556113"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /gene="terL"
                     /locus_tag="VGB68_14550"
     CDS             <1..831
                     /gene="terL"
                     /locus_tag="VGB68_14550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEY0050508.1"
                     /translation="EELDEEFQHIRNSLPEEIRETARREDFIKSVTFIASNVYDNKKL
                     LESNPQYLANLLALPLVERERLLGGNWKVKASAGKVFNRQWFEIVDELPKIDLVCAYW
                     DFAATEKEIKGTKVDGKNDPDFTARTLIGYSKAEKKWYILDVLAVQQNPASVEKLFLE
                     TCKLDRDEAKRLGARFMVRWEIEPGSAAKRESHRLTTQLAGYDAKGKNFKGDKLTRAR
                     SLAVQAEAGNVKCKQGSWNGEFLSHMHGQPDLPHDDIMDSAAGAFNSTVGEGSQGFGF
                     "
     gene            complement(848..1405)
                     /locus_tag="VGB68_14555"
     CDS             complement(848..1405)
                     /locus_tag="VGB68_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050509.1"
                     /translation="MGRTIPFDPSKGIAPARLSALNGEAKKKMDVHAADGSLNFTEFG
                     AMLSDILSGPYNENEKCYIWTRIAHTKGQYVISNVGLRKENIDSYRPGTSAGHVVIGF
                     DDGYHCPLANLSTEDAIAGIKKHEKNEGGAVGAVGRKVFETFGGPPVNIGDEAASMNQ
                     ILALKAFIKEGFPGYVREWRALFLM"
     gene            1585..2940
                     /locus_tag="VGB68_14560"
     CDS             1585..2940
                     /locus_tag="VGB68_14560"
                     /inference="COORDINATES: protein motif:HMM:NF016988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEY0050510.1"
                     /translation="MPIQDIKPALARISNNQSKYKEFYDYYAGRHALNFASDKFKNKF
                     GKRLQKLRENLCKVVVRAPASRLEIINFLADKKNTENPAWKLWKQSKMPLVAGDVHRE
                     AFKTGDAYVIVWVDKKRKVKIYPQLANNITVWKDAETGEITKAAKAWKGDDGFYYLTL
                     YYPDVIEKYVTKGKHETLPLNPAAFDARHIDGESFPLPNTFGRVPVFHFKYDAEMCEQ
                     GNSLLADVIPLNDALNKSWADILGAQEENMRRRRYVAGMQVEIDEETGKKINPFKPDD
                     DILFAVDSETKFGEFSDADLEQMLKVLERAIMSICLVSGIPPSYFNLESTGNAISGDA
                     LTKLEARFIAIIQDAQRSFGETWGEVIDFGMELQNASSEELEIETQWTDAAPVDESEW
                     LDNALKKKSLGWSDEQLQRDFGLSNEQIDKMIKELRAREKAKVEAAAKFFDGGNSIVE
                     E"
     gene            2945..3118
                     /locus_tag="VGB68_14565"
     CDS             2945..3118
                     /locus_tag="VGB68_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050511.1"
                     /translation="MIRSEPTSLSCEPTYPNETDLVMFSIRFPDWTGHLKHKDCEKEC
                     PHKIEDCGLWKDK"
     gene            3115..4056
                     /locus_tag="VGB68_14570"
     CDS             3115..4056
                     /locus_tag="VGB68_14570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEY0050512.1"
                     /translation="MTPEQFAREQREFIIRTEKQSAILLLAEFRELKSEITQFLTTNL
                     TGTITIQKITENAFLERALDEIDVQIERITNPFSRIVTRGQQRVINFAAGALRRFLEI
                     ESDGIFSVDQPAAQKLIGRTQSGTSLSNFFRRLKQPLREKAKEQLIEGFALGESSRKI
                     AKRINDVADTGLARALGISRDSTVYAYRSASIDFYKQTKSVSGFRFMSSLDVRTCIIC
                     WGLHGKIFKLKHKPFVHYQCRCAIIPVFSVENNSIETGIEKFNKLEKGYQKQILSNKR
                     FELFENGNTLESFISMKKSKEFGEIPFIKPLADLVSD"
BASE COUNT         1145 a          988 c         1024 g          938 t
ORIGIN      
        1 gaagaactcg acgaagagtt tcaacacatc agaaactctc tgccggaaga aatccgcgaa
       61 acggcgcggc gcgaagattt cataaaaagc gtcaccttca tcgcgtcgaa tgtttacgac
      121 aataaaaagc tgctcgaatc aaatccgcaa tatctggcga acctgctggc tttgccgctg
      181 gtcgagcgcg agcgtctgct cggcggaaac tggaaagtca aagcgagcgc cggaaaagtc
      241 ttcaatcgtc aatggtttga aatcgtcgat gaactcccga aaatcgacct cgtttgcgct
      301 tactgggact ttgcagcgac ggaaaaagaa atcaaaggca caaaggtgga tggcaagaat
      361 gacccggatt tcaccgcgcg aactttaatc ggatattcaa aggcggaaaa gaaatggtat
      421 atcctcgacg tgctcgccgt gcagcagaat ccggcttcgg tcgaaaagct ttttctggaa
      481 acctgcaagc tcgaccgcga cgaagcgaaa cgcctgggag cgcgttttat ggttcgctgg
      541 gagattgagc ccggaagcgc ggcaaaacgc gaatcgcacc ggttgacgac gcagctcgcc
      601 gggtacgacg ccaagggcaa aaacttcaaa ggcgacaaac tcacgcgtgc gcgctcgctc
      661 gccgtccaag ccgaagccgg aaacgttaaa tgtaaacaag gctcgtggaa cggcgaattt
      721 ttatcgcata tgcacgggca gccggatttg ccgcacgacg atattatgga ctcggcagcc
      781 ggcgcgttta actcgaccgt cggcgaaggc tcgcagggat tcggtttcta agccgataac
      841 cgcattctta catcaaaaag agcgctctcc attcgcggac atagcccgga aatccttctt
      901 tgataaatgc ttttaaagcg agtatctgat tcatcgaagc agcttcatcg ccgatattga
      961 ccggcggtcc gccgaatgtt tcgaaaacct ttctgccgac tgcgccgacc gctccgccct
     1021 cgttcttttc gtgtttcttt atgccggcga tagcgtcttc ggtcgacagg ttcgccagtg
     1081 ggcaatgata gccgtcgtcg aaaccgataa cgacgtgccc cgcagatgtt ccgggacgat
     1141 aggagtcgat gttttccttt ctcagcccaa cattggaaat cacatactgc cccttggtat
     1201 gagcgattcg cgtccagata taacattttt cgttctcgtt atacggtccg cttaaaatat
     1261 cgctcagcat cgcgccgaac tcggtaaagt taagactccc gtcggcggcg tgaacgtcca
     1321 ttttcttttt tgcctcgcca ttgagtgcgg aaaggcgcgc cggcgcgatt cctttgcttg
     1381 gatcgaatgg aatagttctc cccatattat tattctgctc ctgtaagatt agtatttaat
     1441 cgactcgaat tttcggtctc atcattaaag cgaaaaaccg gaacgaaagg tatcaaccgc
     1501 taaacaaccg cttgcaattt ttaattcata atgaggctca aatgtaagcc tcattctttg
     1561 attaactcac ttcactaaat taaaatgcca atccaagaca tcaaacccgc attagctcga
     1621 atcagtaata atcaatccaa atataaagag ttttacgact attacgccgg gcgtcacgcc
     1681 cttaacttcg cctcggacaa gtttaaaaac aaattcggaa agcgcctcca gaagctgcgt
     1741 gaaaacttgt gtaaagtcgt cgtccgcgcg ccggcgtcgc ggctggaaat aatcaatttt
     1801 ttagccgaca aaaagaatac ggaaaatccg gcgtggaaat tgtggaagca gtcgaaaatg
     1861 ccgctcgtcg ccggcgacgt gcaccgcgaa gctttcaaaa ccggcgacgc ctatgtgatt
     1921 gtctgggtcg ataaaaagcg caaggtgaaa atctacccgc agctggcgaa taatatcacc
     1981 gtctggaagg acgccgaaac gggcgagatt accaaagcgg caaaagcgtg gaaaggcgac
     2041 gacggatttt attacctcac gctttattac ccggacgtaa tcgagaaata cgtcaccaaa
     2101 ggaaaacacg aaacgctgcc tttgaatccg gcggctttcg acgcccggca catcgacggc
     2161 gaaagctttc cgctgccgaa cactttcggg cgcgtcccgg tctttcattt caaatacgac
     2221 gcggaaatgt gcgagcaggg aaattctctg ctcgcggacg tcattccttt aaacgacgcg
     2281 ctcaataaat cctgggcgga catcttaggc gctcaggaag aaaatatgcg ccgacggcgt
     2341 tacgtcgccg ggatgcaggt cgaaattgac gaggaaaccg gcaagaaaat caatccgttc
     2401 aaaccggacg acgatatttt attcgccgtg gattccgaga cgaaattcgg cgagttttcc
     2461 gacgcggatt tagagcagat gctcaaagtg ctcgaacgcg ccattatgtc gatttgtctt
     2521 gtttcgggta ttccgccgtc ttatttcaat ctcgaatcga ccggcaacgc catttccggc
     2581 gacgcgctga caaagctcga agcccgcttc atcgcgatta tccaggacgc gcagcgctcg
     2641 tttggcgaaa cgtggggcga ggtgattgat ttcgggatgg agctgcaaaa cgcgagcagc
     2701 gaagaattgg aaatcgaaac gcagtggacg gatgccgccc cggtcgacga aagcgaatgg
     2761 ctcgataacg ctctcaagaa aaaatcgctc ggctggtccg acgagcagtt gcaacgcgat
     2821 ttcggtttga gcaatgagca aatcgacaaa atgataaagg aactcagagc gcgggaaaag
     2881 gcgaaagtcg aagcggcggc gaagtttttc gacggcggaa attcgatagt cgaggagtaa
     2941 aaagatgatt cgctcagagc cgacaagctt aagttgtgag ccaacatatc ctaacgaaac
     3001 ggatttagta atgttttcaa ttcgttttcc ggattggaca ggacatttaa aacataaaga
     3061 ttgtgaaaaa gagtgtcctc ataaaattga agattgcggc ttgtggaaag ataaatgacg
     3121 cctgaacaat ttgccagaga gcaacgcgaa ttcataatca gaaccgaaaa acaatcggcg
     3181 attttactgc tggctgaatt tcgggagttg aaatcggaaa tcacgcagtt tctgacgacg
     3241 aatctgacgg gcacgattac gattcaaaaa atcaccgaaa atgctttttt agaacgcgcg
     3301 ctcgacgaaa tcgacgtgca gatagagcgg atcacgaatc ccttttcccg aatcgtgacg
     3361 cgcgggcagc agcgcgtgat taatttcgcc gcgggcgcgc ttcgaagatt tctggaaatc
     3421 gaatctgacg gcattttcag tgttgatcaa ccggcagctc aaaagctcat cggtcgaacg
     3481 caaagcggca catcgctctc gaatttcttc cggagactta aacagccgct ccgcgaaaag
     3541 gcgaaagaac aattaatcga aggcttcgcg ctcggagaat cgagccggaa aatcgccaaa
     3601 aggattaacg atgtcgccga cacgggtttg gcgcgagcgc tgggaatttc cagagattct
     3661 accgtttacg cttatcgctc ggcttccatt gatttttaca agcaaacaaa atcggtttcc
     3721 ggctttcgtt ttatgtcgtc tttagacgtt cggacgtgca ttatttgctg gggactacac
     3781 ggtaagattt tcaaactgaa acataaaccc tttgtgcatt atcagtgccg atgtgcaatt
     3841 atccctgttt tttccgtcga aaataattcg attgaaacgg gaattgagaa attcaacaaa
     3901 ctggaaaaag gttatcaaaa gcaaattctc agcaacaaaa gatttgagtt atttgaaaac
     3961 ggaaacacgc tggaatcttt tatcagtatg aaaaaatcga aagaatttgg tgagattccg
     4021 tttataaaac cgcttgctga tttggtaagc gactaagaaa tgcctcgcag cgcgtcaggt
     4081 tgtaatcagc ttaaa
//
LOCUS       DASWNE010000746         8009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_273289, whole genome shotgun sequence.
ACCESSION   DASWNE010000746 DASWNE010000000
VERSION     DASWNE010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8009
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273289"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="VGB68_14575"
     CDS             <1..999
                     /locus_tag="VGB68_14575"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0050513.1"
                     /translation="YLIALGYVVNLYQRGTASLKRINEIQETVPTIGDEPSVEVQPSI
                     KGAVEFRNLTFGYNGKPVLKDVDLKIEPGKTVAFVGKTGSGKSTLVSLIPRLMDAPEN
                     TIFVDGISVRRYPLEQLRKSIGFVPQETFLFSDTLAENIAFGVESRESRVERQKMEGG
                     IQKSDVGRQDGANGSSFPTPDSRFEDDPRFDEIKRAAEIAGLANDIESFPYKYEQLVG
                     ERGITLSGGQKQRTAIARAVIRDPRILILDDSLSAVDTYTEETILHNLRDVRENRTTL
                     IVSHRVSTIRDADLICVLEDGRIIERGTHQELVNLNGEYADLYRRQLLEEELAATE"
     gene            1016..1471
                     /locus_tag="VGB68_14580"
     CDS             1016..1471
                     /locus_tag="VGB68_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050514.1"
                     /translation="MNQNPPKTGIETAYRTLVIVWLALLVSQIMLPVVIYFAKPEAFR
                     FDFSKPSLGENPLIIIVFALLAISNLALSFVLSRKNLNQAIAGQNIGLVQTAMIIGCA
                     LCEAISLFGVVLVFAFSYQYFFLWFALGIPGTILHFPKRENLVAAGYKR"
     gene            1650..4427
                     /locus_tag="VGB68_14585"
     CDS             1650..4427
                     /locus_tag="VGB68_14585"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY0050515.1"
                     /translation="MRKAFFLPALVLCLCAWCFAQDDDVTQGTLEAFGKKGNQLGACP
                     LKRTSVKAEISGFLTRVKVIQEFENNFYEPIEAVYTFPLPAGAAVDDMTMKIGERTIR
                     GLIMRREEAQKVYQTAKNEGKTASLIEQERPNIFTQSVANILPNKKIIVEISYVETLK
                     YEDGAYEFVFPMTVAPRYNPASVTPEDAQKISPPVIADSRDGHDISIEVSLNAGVPVE
                     DIRSKTHEIETFNLSPNQASIKLRNQTAIPNKDFVLRYDVSGKRIEDAVLAHRDARGG
                     FFTLILSPPEKFAVEDVAPKEIVFVLDTSGSMEGFPIEKAKESMKMALEGLYPNDTFN
                     LITFAGDTHVLFDEPVPATPDNLRKAQEFLDSRRGDGGTEMMEAVKIALAPSDAPDHM
                     RIVCFMTDGEVGNDTEIINEVKKYRNARVFAFGIGSSVNRYLLDNIAKEGRGEVEYVA
                     LEDDGSAAARRFHERIRNPLLTDVSIDWNGLPVADVYPQKIKDLFSAQPVVVHGRYTR
                     GFKGAIRLRGKVGGQDFVREIPFNLPDAESQNDALATLWARQKIDDLMFANWNAEEEE
                     TRVTPQLKAAVTKIGLDYRLLTSFTSFVAVEEKVVNYRGKPRKVRVPVYAPAGTVSAE
                     DELSTLGTGSGSGTGNGSGSATLPPSNMSVDVTNLTLSQTINGQEVIISSSKSRATMP
                     SATPVQTTGTPVQTTDTPVPKAISGGVVNGKAESLPKPDYPLAARAVRAAGAVSVQVV
                     IGETGSVISAQAVSGHPLLRQASVAAARKAKFQPTLRSGGAVKVTGVIVYNFVIPANS
                     QPQIDATLDKMQLPTEEIKLPEPLAPAAKRQKLIDEKFHLWLADLAARLDKNASPAAA
                     NEAKFVRDGKARLQIWLAEKTPETIEKLKQLGFEIVSEKDNKVFGLISLAKLVALSEI
                     EQVKYVLPNLK"
     gene            4542..5555
                     /locus_tag="VGB68_14590"
     CDS             4542..5555
                     /locus_tag="VGB68_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050516.1"
                     /translation="MLKHYMHNRERYFAMLNANRVVRPFEWGTEFINGSASGDDPRRI
                     FREYSKKVLADSDAFFHLSEINDYRLDGQQLTWTSTIETPSIENNTAYAQLFPHKENK
                     KSAVVVLPHWNAKAKTYFDLCRVFNRVGVTALRLTLPYHEERMPPELDRADYLVAPNV
                     GRTLQSLRQSVVDTRAAVRWLKQQGYEKVGVVGTSIGSCVGFFAFAHDLTIDAGVFNH
                     VSGYVADVVWRGLSTYHVKEGIGNNVSLDELREFWLPISPMVYMDKLAAMPPRPQRYI
                     YTLYDLTFPVDLSRRLMKALRDRDIEHSAVKIPCGHYTLGEKPWVYLDGWKIVSFLRK
                     HLR"
     gene            complement(5589..6461)
                     /locus_tag="VGB68_14595"
     CDS             complement(5589..6461)
                     /locus_tag="VGB68_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050517.1"
                     /translation="MIEKIEFHGANCLLLANGEIELIVSTAYGPRILFYGLGGGENIL
                     GWHPEARVETALGEWRPYGGHRLWIAPENMPLSYAPDNAQVEYSAENDFSARFVQPLE
                     NETKTQKEIVLTLGEKGSEASVEHRITNRGAAEIELSAWALTIMRGGGAAIIPNEPFK
                     PYSGENLLPVRSTAFWSYTDLTDSRWHIGKNFIRLSVDENKPEPQKIGVLNRQGWTAY
                     AWRNLLFVKRFDFIETAAYPDMNSNTEVYTAGSFVEVESLSPLRKLAPGESLVYTERW
                     QLFENVEIDELIEK"
     gene            6739..7689
                     /locus_tag="VGB68_14600"
     CDS             6739..7689
                     /locus_tag="VGB68_14600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0050518.1"
                     /translation="MTALAEKHKFIVEDAAARLLARAENSRRLEAADLSARVSASIEK
                     YVLRDNENASSAEIKEFIDALNADDLCLIIACERNDETAWNDLVTKYDGCVKAAARKI
                     SGNNEDAEDLAGSIWAELYGLKETGGVRRSKLGYYSGRGSLAGWLRAVVSQLAVDEFR
                     KQSKFVQIEEAREFENLANESSENSENSKIVSHSDNPEDVFSEKQTRKDISDALRQAI
                     ARLEAEDRLIMKLYYFDDLKLKDIGAALGFHEATASRKLVRIQTEIRKSVEKILCAEH
                     GWKQEEVKRYLSETASKLDLSIEKLFGWLALLAVVQDFLR"
     gene            7772..>8009
                     /locus_tag="VGB68_14605"
     CDS             7772..>8009
                     /locus_tag="VGB68_14605"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY0050519.1"
                     /translation="MTEEFDKEIDSLLRRAAKGESVQAFDAHMDADEVSLFAENALTA
                     KARARAAEHLAECAKCRKILSHLISFDAEPESETI"
BASE COUNT         1972 a         2087 c         2151 g         1799 t
ORIGIN      
        1 tatctgattg cgctcggcta cgtcgtcaat ctttaccagc gcggcacggc ttccttaaaa
       61 cgcatcaacg agattcagga aaccgtcccg accatcgggg acgagccgag cgtcgaggtt
      121 cagccgtcga ttaaaggcgc tgtcgaattt cgcaacctga ctttcggcta caacggcaag
      181 ccggttttga aagacgtgga tttgaaaatc gagccgggca aaaccgtcgc tttcgtcggc
      241 aaaaccggaa gcggaaaatc gacgctcgtc agcctgattc cgcgcctgat ggacgcgccg
      301 gaaaatacga ttttcgtcga cggcatctcc gtgcgccgct atccgctcga acagcttcgc
      361 aaatcaatcg gctttgtgcc gcaggaaact tttctgttca gcgacacgct ggcggaaaat
      421 atcgctttcg gagtcgagag ccgggaatcg agagtcgaga ggcaaaagat ggaaggcgga
      481 attcagaaat cggatgtcgg gcgtcaggac ggcgcgaacg gctccagttt cccgacgccc
      541 gactctcgct ttgaagacga cccgcgattc gatgaaatca aacgcgccgc cgaaattgcg
      601 ggtctggcaa acgacatcga aagctttccg tataaatacg aacagcttgt cggcgagcgc
      661 ggcatcacgc tttccggcgg acagaaacag cgcacggcga tcgcccgcgc cgtaattcgc
      721 gacccgcgca ttctgattct ggacgactcg ctttcggcgg tcgataccta cacggaagaa
      781 acgattttgc acaatctgcg cgatgtgcgg gaaaaccgca cgaccttgat tgtctcgcac
      841 cgcgtttcga cgattcgcga tgcggatttg atttgcgttc tcgaagacgg gcgcataatc
      901 gagcgcggaa cgcaccagga attggtaaat ctcaacggcg aatacgccga cctttatcgt
      961 cgccagcttc tcgaagaaga gcttgcggca accgaataaa aaggagattt ttgaaatgaa
     1021 tcaaaatccg ccgaaaaccg gcatcgagac ggcttatcgc acgctggtca tcgtctggtt
     1081 ggcgctgctt gtttcgcaaa ttatgcttcc ggtcgttatt tactttgcca aacccgaagc
     1141 gttcagattc gacttttcaa aaccgtcgct cggcgaaaac ccgttgataa ttatcgtttt
     1201 tgccctgctg gcgatttcga atctcgcgct ttcgtttgtg ctgagccgga aaaatctgaa
     1261 tcaggcgatt gccgggcaaa acatcggtct ggtgcagacg gcgatgatta tcggctgcgc
     1321 tttgtgcgaa gcgatttctc ttttcggcgt ggttctggtt ttcgctttct cgtatcaata
     1381 tttcttttta tggtttgcgc tcggcattcc gggaacgatt ctgcattttc cgaaacgcga
     1441 gaatctcgtc gccgccggct acaaaagata aagggcgaaa aatcgcaaca aaattttaaa
     1501 atcagcgtgc aaaaccgacg gcggtcgatt ttattttgca cgccgaattt ttttctcgcg
     1561 ctcagggaac caattgccgg gttgcggtgt ctaagctgtt gtagtccgcg tactccaaaa
     1621 tcaaaaactt gtctcaaagg agaaaattta tgcgaaaagc tttctttttg ccggcgctcg
     1681 ttttgtgtct gtgcgcctgg tgtttcgctc aggatgacga tgtaacgcag ggaacgctcg
     1741 aagccttcgg taaaaaaggc aaccagctcg gcgcgtgtcc tttaaagcgc acttcggtca
     1801 aagccgaaat cagcggtttt ctgactcgcg tcaaagtaat tcaggagttt gaaaataatt
     1861 tctacgaacc aatcgaagcc gtttacacgt ttcctctgcc ggcgggcgcg gcggtcgatg
     1921 atatgactat gaaaatcggc gaaaggacga ttcgcggtct gattatgcgg cgcgaagaag
     1981 cgcagaaagt ttatcaaacg gcaaaaaacg aaggcaaaac ggcgagcctg atcgagcagg
     2041 aacgcccgaa tatttttacg caatcggtcg ccaatatact gccgaataaa aaaatcatcg
     2101 tcgaaatcag ctacgtcgaa accttaaaat atgaagacgg cgcgtatgaa ttcgtgtttc
     2161 cgatgacggt cgcgccgcgt tataatccgg cttcagttac gccggaggac gcgcaaaaaa
     2221 tctcgccgcc cgtgattgcc gacagtcgcg acggacacga tatttccatc gaagtcagcc
     2281 tgaatgcggg cgttccggtc gaagacatcc gctcgaaaac gcacgaaatc gaaacgttta
     2341 acctgagccc gaatcaggcg agtatcaagc tcagaaatca aaccgcaatt ccgaacaagg
     2401 atttcgtttt gcgttacgac gtgtcgggaa agcgcatcga agacgccgtg ctggcgcacc
     2461 gcgacgcgcg cggcggattt ttcactttaa ttctgtcgcc gcccgaaaaa tttgcggtcg
     2521 aagacgtcgc gccgaaagag attgttttcg tcctcgacac gtcgggctcg atggaaggct
     2581 ttccgattga aaaagccaaa gaatcgatga aaatggcgct cgaagggctt tacccgaacg
     2641 acacttttaa tttaataact ttcgcgggcg acacgcacgt tttgttcgac gaaccggttc
     2701 cggcaacgcc cgataatctt cgcaaagcgc aggaatttct ggattcgcgg cgcggcgacg
     2761 gcggcacgga aatgatggaa gccgtcaaga ttgcgctcgc gccttcggac gcgccggacc
     2821 atatgcgcat cgtctgtttt atgacggacg gcgaggtcgg caacgatacg gaaatcatca
     2881 atgaagtcaa aaaatacagg aacgcgagag ttttcgcttt cggcataggc agctcggtca
     2941 atcgctattt gctggataat atcgcgaaag aagggcgagg cgaggtcgaa tacgtggcgc
     3001 tcgaagacga cggctcagcc gccgcgcggc gttttcacga aagaatccgc aatccgcttt
     3061 tgacggacgt ctcgattgat tggaacggtt tgcccgtcgc ggacgtttat ccgcagaaaa
     3121 tcaaagattt atttagcgcc cagccggtcg tcgttcacgg tcgctacacg cgcggcttca
     3181 agggtgcgat tcgcctgcgc ggaaaggtcg gcgggcagga tttcgtgcgc gagattccct
     3241 ttaatctgcc cgacgccgaa tcgcaaaacg acgcgctggc gacgctctgg gcacgacaaa
     3301 aaatcgacga tttgatgttt gccaactgga atgcggaaga agaggaaacg cgcgtcacgc
     3361 cgcaactgaa agccgccgtg actaaaatcg gattggatta ccgcctgctg acaagcttta
     3421 cttcgttcgt cgccgtcgaa gaaaaagtag tcaattatcg cggcaaaccg cgcaaagtcc
     3481 gcgttccggt ttacgcgccg gcgggaaccg tttccgccga agatgagctt tcgacgctcg
     3541 gaaccggttc gggttcggga acaggcaacg gcagcggttc ggcgacgctg cctccaagca
     3601 atatgtctgt cgatgtgacg aatttaacgc tttcgcaaac catcaatgga caggaagtga
     3661 ttataagctc ttcaaaaagc agagcgacga tgccgagcgc cacgcctgtt caaacgacgg
     3721 gcacgcccgt tcaaacgacg gacacgcccg ttccgaaagc tatttcgggc ggcgtggtca
     3781 acggaaaagc cgagagttta ccgaaacccg attacccttt agccgccaga gcggttcggg
     3841 cggcgggcgc ggtttccgtg caggtcgtca tcggcgaaac gggcagcgtg atttccgcgc
     3901 aagccgtcag cggtcatcct ttgcttcggc aggcgtcggt cgcggcggcg cgcaaggcaa
     3961 aattccagcc gacgctccgt tccggcggcg ctgtgaaagt gacgggcgtg attgtctata
     4021 atttcgttat tccggcaaat tcgcagccgc aaatcgacgc gacgctcgac aaaatgcagc
     4081 tgccgaccga agaaataaaa ctgcccgaac cgctcgcgcc cgccgcgaaa cggcaaaagc
     4141 tgattgacga aaagtttcac ctgtggctgg cggatttggc ggcgcgtctg gacaaaaacg
     4201 cctcgccggc ggcagcaaac gaagcgaaat ttgttcgcga cggcaaagcc cggctgcaga
     4261 tttggctggc ggaaaaaacg cccgaaacga ttgagaaact aaagcagctc ggttttgaaa
     4321 tcgtgtctga aaaggacaat aaagtcttcg gactaatttc tctggcgaaa ctcgtcgccc
     4381 tgtctgaaat cgaacaggtg aaatacgttc tgcccaatct taaataagtc cggagagtcc
     4441 ggagagtccg gagcccggag agtcgaaaga aaagttcgat ttgactcgcg actcccggac
     4501 tctctggact ctccacacta ttttccttac cataaatgtt tatgcttaaa cattatatgc
     4561 acaaccgcga gcgctatttt gcgatgctta acgccaatcg cgtcgtgcgt ccgtttgaat
     4621 ggggaacgga atttatcaac ggcagcgcta gcggcgacga cccgcgccga attttccgcg
     4681 aatactcgaa aaaggttctg gcagacagcg acgcgttttt tcatttgtcc gaaattaacg
     4741 attaccggct cgacggtcag caactgacct ggacgagcac gattgaaacg ccttcgattg
     4801 aaaacaatac ggcttacgct caactgtttc cgcataaaga aaataaaaaa tcggcggtcg
     4861 tcgtgctgcc gcattggaat gccaaggcga aaacctattt cgatttgtgc agggttttta
     4921 accgcgtcgg cgtgaccgcc ttaagactga cgctgccgta tcacgaagag cgaatgccgc
     4981 cggaactcga ccgcgccgat tatctggtcg cgccgaatgt cgggcgcact ttgcagtctt
     5041 taaggcaatc ggtcgtcgat acgcgcgcgg cggtgcgctg gctgaaacag cagggttatg
     5101 aaaaagtcgg cgtcgtcggg acgagcatcg gctcctgcgt cggatttttt gctttcgctc
     5161 acgatttgac gattgacgcg ggcgttttca atcacgtttc cggatacgtc gccgacgtcg
     5221 tctggcgcgg gctttcgact tatcacgtca aggaaggcat cggaaataat gtttcgctcg
     5281 atgaattgcg cgagttctgg ctgccgattt cgccgatggt ttatatggac aagctcgccg
     5341 ctatgccgcc gcgcccgcag cgctacattt acacgcttta cgatttgacg tttccggtcg
     5401 atttgtcgcg ccgtctgatg aaagccctgc gcgaccgcga catcgaacac agcgccgtca
     5461 aaattccctg cgggcattac actctgggcg aaaagccgtg ggtttatctg gacggctgga
     5521 aaattgtcag ttttttgcga aagcacctgc gctgaaaaag cggcaatcca tttttttccg
     5581 aaacattttt acttttcaat caattcgtca atctcgacat tctcaaacag ctgccagcgt
     5641 tccgtgtaaa cgagcgattc gcccggcgcg agcttccgca aaggcgaaag cgattcgact
     5701 tcgacgaatg aaccggcggt gtaaacttcc gtgttcgagt tcatatcggg ataagcggca
     5761 gtttcgatga aatcgaatct tttgacgaac aataaattac gccacgcgta agccgtccag
     5821 ccctgccggt tcaaaacccc gattttctgc ggctcgggct tgttttcatc gacgctcagg
     5881 cgaataaaat ttttcccgat gtgccagcgc gaatcggtca aatccgtgta tgaccagaaa
     5941 gccgtcgagc gcacgggcaa aagattttcg ccgctgtaag gcttgaaagg ctcgttcggg
     6001 attatcgccg cgccgccgcc gcgcataatc gtcaaagccc acgccgacaa ttcgatttcc
     6061 gccgcgcccc gattcgtaat gcgatgctcg acgctcgctt cgcttccctt ttcgccgagc
     6121 gtcagcacaa tttccttctg cgttttcgtc tcgttttcga gcggctgaac gaaacgcgcg
     6181 gaaaaatcgt tttccgcaga atattcgacc tgcgcattgt ccggcgcgta ggaaagcggc
     6241 atattttccg gcgcaatcca caggcgatgc ccgccgtaag gtcgccattc gccgagcgct
     6301 gtttcgacgc gcgcttccgg gtgccagcct aaaatgtttt ccccgccgcc gagcccgtaa
     6361 aataaaattc gcggaccgta tgcggtcgat acgattaatt cgatttcgcc gttggcgaga
     6421 agcaggcagt ttgccccgtg aaattcaatt ttttcaatca ttcgattatt ataacgaacc
     6481 gcgcgccgag tttcatttgt cgagcgggcg aaaacgaatt acgaattacg aattacgaat
     6541 tacgaatgaa agattgtttc ttctaattcg taattcgtaa ttgataattc gtaattcgta
     6601 attcaaagaa agcgtttccg cctgctcgcc cggcgtcatt aattattgat taaaataaaa
     6661 cgttatcgct catctgcaaa cttttagccg tgtcaaacgc atccaatttc tgctaaactc
     6721 tttccgattc gctaacagat gaccgcgctt gccgaaaaac ataaattcat tgtcgaagac
     6781 gccgccgcgc gactgctcgc ccgcgccgaa aattcgcggc ggctggaagc ggcggattta
     6841 tcggctcgcg tttcggcgtc aatcgaaaaa tacgttttgc gcgataacga aaacgcttcg
     6901 agcgcggaga taaaagaatt tatcgacgcg cttaacgccg acgatttatg tctgattatc
     6961 gcctgcgagc gcaacgacga gacggcgtgg aacgatttgg tgacgaaata cgacggatgc
     7021 gtcaaagcgg cggcgcgaaa gatttcgggc aataacgaag acgccgaaga ccttgccggc
     7081 tcaatctggg cggagcttta cggcttaaag gaaacgggcg gcgtcaggcg cagcaagctc
     7141 ggttattatt cgggtcgcgg ctcgctcgcg ggctggcttc gcgccgtcgt ttcgcaattg
     7201 gcggtcgacg aatttcgcaa gcagtcgaag tttgtccaga ttgaagaggc gcgcgagttt
     7261 gaaaatctgg cgaacgaatc ttcggaaaac tcggaaaatt caaaaatcgt ttcgcacagc
     7321 gacaacccgg aagacgtttt cagcgaaaaa caaacgcgca aagatatttc cgacgcgctc
     7381 cggcaggcga ttgcgcggct cgaagccgaa gacaggctga ttatgaagct ttattatttc
     7441 gacgatttaa aacttaaaga catcggcgcg gctctgggtt ttcacgaagc gaccgccagc
     7501 cgaaagctcg tccgcatcca gacggaaatt cgcaaatcgg tcgaaaaaat tctttgcgcc
     7561 gaacacggct ggaaacagga agaagtcaaa cgctatctgt cggaaacggc atccaagctg
     7621 gacttgagca tcgaaaaatt attcggctgg ctggcgcttc tggctgtcgt gcaagatttt
     7681 ttacgctgag gcgttctgac aatggcattg aacattgagc atttatcatc gaacgataag
     7741 cagccgaaaa atgataaatg ttaaccgata aatgacggaa gaattcgaca aggaaataga
     7801 ttctcttttg cgccgggcgg cgaaaggcga aagcgtgcag gctttcgacg cgcatatgga
     7861 cgcggacgaa gtttcgcttt ttgccgagaa cgccttgacg gcgaaagcgc gcgcccgcgc
     7921 cgccgagcat ctggcggaat gcgcgaagtg tcgaaaaatt ttgtcgcatc tgatttcatt
     7981 cgatgccgag cctgaaagtg aaacaattc
//
LOCUS       DASWNE010000747         3134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_236053, whole genome shotgun sequence.
ACCESSION   DASWNE010000747 DASWNE010000000
VERSION     DASWNE010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236053"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1156)
                     /locus_tag="VGB68_14610"
     CDS             complement(<1..1156)
                     /locus_tag="VGB68_14610"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0050520.1"
                     /translation="MKKLSTGFLLLVFIFVHAAPAQERLDEQALARIKAEGFQNSKIM
                     ETLSYVTDVFGGRLTNSPNLKNAQNWTRDRMISWGFENVGLESWGNWGKGWSVDKFSV
                     EMFEPTYDRLNAYPLAWTPSTSGAVSGNPLVVSIRSNADFAKYRGKLKGAVVLNGRFD
                     LNSPESRFQTPSRRFTDEELARASLVINPAKDGEINGGATTNYRDEEKDWQVGLARGK
                     EITKFFKDEGVAALILPSSRANGVLSVQGYYETDSSKNVPTFVLAREQYARIVRLNDK
                     NIAVRLELNLQARGHEDLAGYNVVGEIVGSDSRIKDEVVMLGGHFDSWHAGSGATDNA
                     AGCVVMMEALRILKKIGAKPRRTIRVALWSGEEQDYGGSVGYVRKHFGDPL"
     gene            complement(1354..>3134)
                     /locus_tag="VGB68_14615"
     CDS             complement(1354..>3134)
                     /locus_tag="VGB68_14615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEY0050521.1"
                     /translation="LLSKFEETLFIDGEAFELSSNQYPNKIYPEGFRYLKSFRLAPFP
                     VWNFEVNGIEIEKKVFMIDGENSTVCQWSVKDRRPQTADRRPEIKLELKPLLSFCDYH
                     ALQRENADFNPDYEVSENSVSIKPEDEMPALFFNHNARSVEKSGFWYRNFEYAIERER
                     GFDFREDLFQPFVLKFDLSETAVLIASTQKCDWRDAGNFEKSEILRREKLIEKAEAKD
                     DFTKQLVLAADQFVVARGAGSSVIAGYHWFSDWGRDTMIALSGLTLATNRAEIAKSIL
                     LEFSGHISEGMLPNRFPDSSEEAEYNTVDATLWYFEAIRAYAEKTGDYDFVRESLYEK
                     LAGIIVCHLRGTRYDIHVDTDGLLYAGTGGVQLTWMDAKIGDLVITPRIGKAVEIQAL
                     WYNALKIMADFAERFGDAEDKARYEAMAEAARYSFNQIFWNEPGECLFDVVINGQRDV
                     SVRPNQIFAVSLPHSMIEAERARKIVEKVEAELLTPVGLRSLSPKDAQYCPIYTGSPF
                     DRDSAYHQGTVWGWLIGAFVDAHRKVYPDGESRVGEILENFKNHLTEAGLGQISEIFD
                     AEAPFKPRGCIAQAWSVAEVLRVINS"
BASE COUNT          736 a          866 c          768 g          764 t
ORIGIN      
        1 tgagcgggtc gccgaaatgt tttcgcacgt agccgaccga gccgccgtaa tcctgttctt
       61 cgccgctcca taaggcgacg cgaatcgtcc ggcgcggctt tgcgccgatt ttctttaaaa
      121 tccgcagcgc ttccatcatc accacgcatc cggcggcgtt gtcggtcgcg ccgctgcccg
      181 cgtgccacga atcgaaatgc ccgcccagca tcacgacttc gtctttaatg cgcgagtcgc
      241 tgccgacgat ttcgccgacg acgttgtaac ccgctaaatc ttcgtgaccg cgcgcctgca
      301 gattcagttc cagcctgacg gcgatgtttt tatcgtttaa gcggacgatt cgcgcgtatt
      361 gctcgcgggc gaggacaaaa gtcggaacgt ttttgctcga atcggtttcg taataccctt
      421 gcacgctcaa aactccgttt gcgcggctgc tcggcagaat cagggcggcg acgccttcgt
      481 ccttaaaaaa tttcgttatt tcctttcctc gcgccagccc gacctgccag tccttttcct
      541 cgtcccggta attagtcgtc gcgccgccgt tgatttcgcc gtctttggct ggatttatga
      601 ccagcgacgc tcgggcgagc tcttcgtcgg taaagcgcct tgaaggcgtc tgaaaccgcg
      661 actcgggcga atttaaatca aatcgaccgt tcaaaacgac agcgcctttc agcttgccgc
      721 gatatttggc gaaatcggcg tttgagcgaa tggaaacaac gagcggattt cccgaaaccg
      781 cgccgctggt cgaaggcgtc caagccagag gataagcgtt cagacgatca taggtcggct
      841 caaacatttc gaccgaaaat ttatcgacgc tccagccttt gccccagttt ccccacgatt
      901 cgagcccgac gttctcgaag ccccacgaaa tcatccggtc gcgcgtccag ttctgcgcgt
      961 ttttcagatt cggcgaattc gtcagtcgcc cgccgaaaac gtccgtcaca tacgaaagcg
     1021 tttccataat tttggaattc tgaaatcctt ccgccttaat ccgcgccagc gcctgctcgt
     1081 cgagtctttc ctgcgccgga gcggcgtgaa caaaaataaa aactaaaagt aaaaagcctg
     1141 tcgataattt tttcatagcg tttttccgaa aatttaatta aatgggagat tacaacagat
     1201 tgcctgcaaa atatcgtcat cgtcaaaaat ggggattttt aaagaagcaa acagatggct
     1261 gtcgtttgag aaaatacgcg gaaaacgaca aaaagttttg aattttgagt ttgtgattca
     1321 acgaattatt caaaattcaa aactcaaaat caatcaggaa tttatgacgc gcaagacttc
     1381 cgccacgctc caagcctgcg cgatgcagcc gcgcggcttg aaaggagcct cggcatcgaa
     1441 aatttcggag atttgaccga gcccggcttc ggtcagatga tttttaaaat tttctaaaat
     1501 ctcgccgaca cggctttcac cgtcgggata aactttgcgg tgagcgtcga caaaagcgcc
     1561 gatgagccag ccccagaccg tgccctggtg ataggcggaa tcgcggtcga aaggcgagcc
     1621 ggtataaatc gggcaatatt gcgcgtcttt cggcgaaagc gagcgcaagc cgaccggcgt
     1681 taaaagctcg gcttcgactt tttcgacgat ttttcgggcg cgttccgctt caatcatcga
     1741 atgcggcagg ctgacggcga aaatctggtt tggtctgacc gaaacgtcgc gctgcccgtt
     1801 tatcacgaca tcaaacagac actcgcccgg ttcgttccag aaaatctgat taaagctgta
     1861 acgcgccgct tccgccatcg cttcataacg cgctttgtct tccgcgtcgc cgaagcgctc
     1921 ggcaaaatcc gccataattt tcagcgcgtt ataccacagc gcctgaatct cgaccgcttt
     1981 tccgatgcgc ggcgtaatca ccaaatcgcc gattttcgcg tccatccagg tcagctgcac
     2041 gcctccggtt ccggcgtaaa gcaagccgtc cgtatcgacg tgaatgtcgt agcgcgtgcc
     2101 ccgcagatga cagacgatta taccggcgag cttttcgtaa agactttcgc ggacgaaatc
     2161 gtaatctccg gtcttttcgg cgtaagcgcg aatcgcttca aaataccaga gcgtcgcatc
     2221 gaccgtgttg tattcggctt cttcgctgga atcgggaaag cgattcggca gcattccctc
     2281 ggaaatgtgt ccggaaaatt cgagcagaat acttttcgca atctcggcgc ggtttgtcgc
     2341 cagcgtcaag ccgctgagcg caatcatcgt gtcgcgtccc caatcggaaa accagtgata
     2401 accggcgata acgctgctgc ccgcgccgcg cgcgacgacg aactggtcag ccgccagcac
     2461 cagctgtttt gtgaaatcgt ctttcgcttc cgctttttcg attaactttt cgcggcgcag
     2521 gatttcggat ttttcaaaat ttccggcgtc gcgccaatcg catttttgcg tcgaggcgat
     2581 gagaaccgcc gtttcgctca aatcaaattt caaaacaaac ggctgaaaca agtcttcgcg
     2641 gaaatcaaag ccgcgctcgc gctcgatcgc gtattcaaaa ttccgatacc agaaaccgct
     2701 tttctcgacg cttcgcgcat tgtgattaaa aaaaagcgcg ggcatttcat cttcgggctt
     2761 aatcgaaacg ctgttttccg aaacttcgta atccgggtta aaatcagcgt tttcgcgctg
     2821 tagggcgtga taatcgcaaa aggataaaag cggctttaat tcaagcttga tttccggtct
     2881 gcggtctgcg gtctgcggtc tgcggtcttt taccgaccac tgacaaaccg tcgaattttc
     2941 gccgtcaatc ataaaaactt ttttctcgat ttcgattccg ttcacttcga aattccaaac
     3001 gggaaacggc gcgaggcgaa acgattttaa atatcgaaag ccttcgggat aaattttgtt
     3061 cggatattga ttcgaggaaa gctcaaacgc ctcgccgtcg ataaaaagag tttcttcaaa
     3121 cttggaaagc agcg
//
LOCUS       DASWNE010000748         4008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_94708, whole genome shotgun sequence.
ACCESSION   DASWNE010000748 DASWNE010000000
VERSION     DASWNE010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94708"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VGB68_14620"
     CDS             <1..642
                     /locus_tag="VGB68_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050522.1"
                     /translation="PENNANEYTQQDFSFQPSVEPQVSTNEYRFETPQQFETAPAFES
                     APTETFESYAPPVEAEAYAPVQEETSYGSFETVADKPQNGFGEAVETMTAAQPVQAAA
                     PVRTRFSERNVDLPIEVTEDERRLHNDARRFARLLVSEIKLYNEQKVKEGREANDLYE
                     RLREAIDRSREMYDKRVQPSVAAKFDYFHYELVNSLGDGEETRLGASYPGAVV"
     gene            complement(804..2897)
                     /locus_tag="VGB68_14625"
     CDS             complement(804..2897)
                     /locus_tag="VGB68_14625"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0050523.1"
                     /translation="MKKSIPSLLLIFLFLTTSVFSQDAKPTAPPIDDDVVKISTTLIQ
                     IDVSVTDKSGKIVSDLKPEDFEIYENGKKQDITNFSFIFTNQRQPEPSSTRNKNREKL
                     PDLPPATLRPEQVKRTIALVVDDITLSFSSTFFVREALKKFVDEQMQDDDLVAIIRTG
                     GGIGALQQFSYDRRQLRAAIEKIRWNPSGMGNIGTFTSIQDGSEPKDDVNEFRESIFA
                     SGTLGALNYVIRGMRDLPGRKSVMLFSDGLPLFITGANGMRQPSRILDSLRRLTDLAN
                     RASVVIYTMDAKGLEVGGLTAEDNTYGVSAAQVEQKLSARRDALFNSQEGLIYLARQT
                     GGLPIINQSDINKGIRRMLDDQKGYYLLGYQPDSETFDPKTRRYNKLEVKLKREDLRV
                     RYRSGFFGITDEQARPSNQTAPQIITAALTSPFAANDITLRLNAVFGNDASAGSFVNS
                     FLHIDANDLKFEKQPDGNHRAIFNLVAMSFGDNGLVVDELSRKYTMTMKEETYRRVLE
                     TGFVYNFTFPVKKPGAYQFRVVLHDSATNKVGSANQFIEVPNLKKERLTLSGIALDNS
                     GSDQREALNQSPTTEKGIDNMTATSLRRFRRGTIVRYGYEIYNAKSGKTQKPQITTQA
                     KVFRDGKVVFEGKPMTLDVSKQTDFRRLFATGILNFSNQTLPGDYVLQIVVTDNLAKE
                     KRRLATQYLPFEIVE"
     gene            complement(3116..>4008)
                     /locus_tag="VGB68_14630"
     CDS             complement(3116..>4008)
                     /locus_tag="VGB68_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050524.1"
                     /translation="TDEMTDNVKQTPQQQILNALLSPFAAGGINLRLNALFGNEAKSG
                     SFVRSFLHINAKDLKFTDEADGSHKATFDILGVSFGDNGIAVDQIGKTYTIVAKDETY
                     EQILRDGFVYSFVFPIKNPGAYQMRVALRDRNSEKVGSANQFIEVPNLKKERLTLSGI
                     VLENLTPAQWQKLAQERSEPVLTNPQSDTSLRRFKRGTVLRYAYEIYNIRLDAAQKPQ
                     AQTQMRIFRDGKPLLEGRQSALDVAGQTNMQKIAAGGAMSLGSALPAGDYVLQIIVTD
                     NLAKEKHRTTTQFVQFEIVE"
BASE COUNT          924 a         1044 c         1045 g          995 t
ORIGIN      
        1 ccggaaaaca acgccaacga atacacgcag caggattttt cttttcagcc gagcgtagag
       61 ccgcaggttt cgaccaatga ataccggttt gaaaccccgc agcaatttga aaccgcgccg
      121 gcttttgaat ctgcgccgac ggaaacgttt gaaagttacg cgccgccggt cgaagcggaa
      181 gcatacgcgc ccgttcagga agaaacgtct tacggttctt ttgagaccgt cgcggacaag
      241 ccgcagaacg gattcggcga ggcggtcgaa actatgacgg cggcacagcc cgttcaggcg
      301 gcagcgccgg tgcgcactcg tttcagcgag cgcaacgtcg atttgccgat tgaagtcacg
      361 gaagacgagc gccggctgca caacgacgcg cgacgttttg cgcgccttct ggtctcggaa
      421 atcaagcttt acaacgagca gaaagtaaaa gaaggacgcg aggcgaacga cctttacgaa
      481 cgcctgcgcg aagccatcga ccgctcgcgc gagatgtatg acaaacgcgt tcagccgtcg
      541 gtcgccgcca aattcgatta ttttcattac gaactggtca actcgctcgg cgacggcgag
      601 gaaaccaggc tcggcgcaag ctatccgggc gcagtcgttt agataaaaac acagcaatcg
      661 aaaaagccgt ctgacgtaaa aatcggacgg ctttttttat ttgcgcgcag aatgcaaaat
      721 taaatttagc tgcgtttgga aaaagatgcg cctgcattcg ctgtcacgaa gcttcaagcc
      781 cggcggacgc cggattcaga atgttactcg acaatctcaa acggcagata ttgcgtcgcg
      841 agccggcgtt tttcttttgc cagattgtcc gtcacgacga tttgcagaac ataatcgccg
      901 ggtaaagtct gattactaaa atttaaaatg ccggtcgcga ataatctccg gaaatcggtt
      961 tgtttcgaaa catcgagcgt catcggtttg ccttcaaaaa ccactttgcc gtcgcggaac
     1021 actttcgcct gcgtcgtaat ctgcggcttc tgcgttttgc cggatttggc gttataaatt
     1081 tcgtagccgt agcgcacaat cgtgccgcgc cggaagcggc gcagcgacgt ggcggtcata
     1141 ttgtcaatgc ctttttccgt ggtcggcgat tggttcagcg cctcgcgctg gtcgctgccg
     1201 gaattatcca gagcgatgcc ggaaagcgtc agacgttctt ttttcaaatt cggaacttcg
     1261 ataaattgat ttgccgagcc gactttgttg gtcgcgctgt cgtgcagcac gacgcgaaac
     1321 tgatacgcgc ccggcttttt gaccggaaag gtgaaattgt aaacaaaacc ggtttccaga
     1381 acgcggcgat aagtttcttc cttcatcgtc atcgtgtatt ttcgggaaag ctcgtcaacg
     1441 accaaaccgt tatcgccgaa gctcatcgcc actaaattaa aaatcgcccg gtgatttccg
     1501 tccggctgtt tttcgaattt caaatcgttg gcgtcgatat gcagaaacga attgacaaac
     1561 gaacccgccg acgcgtcgtt gccgaagacg gcattcaatc gcagcgtaat atcgttggcg
     1621 gcgaaaggcg acgtcaaagc cgccgtgatg atttgcgggg cggtttgatt tgacgggcgc
     1681 gcctgttcgt ccgtaatgcc gaaaaatccg ctccggtagc gaacccttaa atcttcgcgc
     1741 ttgagcttga cttcgagttt gttataacgc cgcgtcttcg ggtcgaaagt ttccgaatcg
     1801 ggctgataac cgagcagata ataacctttc tggtcgtcga gcatccggcg aattccctta
     1861 tttatatcgc tctgattaat aatcggcaga ccgcccgttt gccgcgccag ataaatcaag
     1921 ccttcctgcg aattgaacag cgcatcgcgg cgggcggaaa gtttttgctc gacctgcgcg
     1981 gcggaaacgc cgtaagtatt gtcttccgcc gttaagccgc cgacttccaa gcctttcgcg
     2041 tccatcgtat aaatcacgac cgacgcgcga ttagccaaat cggtcaatct tctgagcgag
     2101 tccaaaatcc gcgacggctg cctcatcccg tttgcgccgg tgataaaaag aggcaagccg
     2161 tccgagaaaa gcatcacgct tttgcgtccg ggcaaatcgc gcattccgcg aatgacgtaa
     2221 ttgagcgcgc cgagcgttcc gctggcaaag atgctttcgc gaaactcgtt aacgtcgtcc
     2281 ttcggctcgc tgccgtcctg tatcgacgtg aaggttccga tgtttcccat tccggacgga
     2341 ttccagcgaa ttttttcgat agccgcccgc agctggcgtc tgtcgtatga aaattgctgg
     2401 agcgcgccga tgccgccgcc ggtgcgaata atggcgacca ggtcgtcgtc ctgcatttgc
     2461 tcgtcgacaa atttctttaa tgcttctctg acgaaaaaag tgcttgaaaa tgaaagcgtt
     2521 atgtcgtcga cgacgagcgc gatggtgcgc ttgacctgtt cgggtctgag cgtagccgga
     2581 ggcaaatccg gcagcttctc gcggtttttg ttccgtgtcg aagacggttc cggttgacgc
     2641 tgattggtaa aaataaaaga aaagttggtt atatcctgct ttttgccgtt ttcgtaaatc
     2701 tcgaaatctt ccggctttaa atcgctgact atttttcccg atttatcggt caccgagaca
     2761 tctatctgaa tcagcgtggt ggaaatttta accacgtcgt catcaatcgg cggcgcggtc
     2821 ggtttggcgt cctgagaaaa aacgctcgtc gttaaaaaga gaaaaatcag taaaagcgaa
     2881 ggaatggatt ttttcattgc agctcctgaa ctaatgaata ttttgcgctt atatatgaga
     2941 gatacggcgc ttttgaaaaa tacattttaa gtaatcttta gaaaaacgcg aagccgcgaa
     3001 actttaaaag gcatctttta ttttttcgac gccaaaccgc tcgcgcgccc gcaaagcgac
     3061 gacaaagcta aatttttgat tgccgccggt tttcggtctg attaagcttt aatttttatt
     3121 cgacaatctc aaactgtacg aactgagtcg tcgtgcggtg tttttccttt gccagattgt
     3181 cggtcacgat aatctgcaga acgtaatcgc cggcgggcag agcgcttccc agactcatcg
     3241 cgccgcccgc cgcgattttc tgcatattgg tctgtccggc aacgtcgagc gcggattgcc
     3301 tgccttccag aagcggcttg ccgtcgcgaa aaatcctcat ctgcgtctgc gcctgcggct
     3361 tttgcgcggc gtcgagcctg atattgtaga tttcgtaggc atagcgcaag accgttccgc
     3421 gcttgaaacg gcgcagagaa gtgtccgact gcggattggt taaaaccggc tcgctccttt
     3481 cctgcgcgag cttctgccat tgcgcgggcg ttaaattttc caggacgatt cccgaaagcg
     3541 tcaggcgttc ttttttcaga ttcgggactt cgataaactg gtttgccgag ccgactttct
     3601 ccgaattccg gtcgcgcagg gcgacgcgca tctgatacgc gcccggattt ttaatcggga
     3661 aaacaaagct gtaaacaaag ccgtcgcgca aaatctgctc gtaagtttcg tctttcgcca
     3721 caatcgtgta agttttgcct atctggtcga ccgctatgcc gttgtcgccg aagctgactc
     3781 ccagaatgtc gaaagtcgct ttatggcttc cgtcggcttc gtcggtaaat ttcaaatctt
     3841 tggcgttgat gtgcaggaac gaacgaacaa aagaaccgct tttcgcttcg tttccgaaca
     3901 gcgcgttcag ccgcagattg atgccgccgg cggcaaaagg cgaaagcagc gcatttaaaa
     3961 tctgctgttg cggcgtctgt ttgacgttat ccgtcatttc atcggtga
//
LOCUS       DASWNE010000749         1792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_446503, whole genome shotgun sequence.
ACCESSION   DASWNE010000749 DASWNE010000000
VERSION     DASWNE010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446503"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VGB68_14635"
     CDS             <1..204
                     /locus_tag="VGB68_14635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050525.1"
                     /translation="QRLNNFSRRGSRNYYRGGYVEPLIGGGAYAESDSESRLSYLEQQ
                     RAGLIAEWQALEEEARRAGVRID"
     gene            285..1754
                     /gene="gatB"
                     /locus_tag="VGB68_14640"
     CDS             285..1754
                     /gene="gatB"
                     /locus_tag="VGB68_14640"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEY0050526.1"
                     /translation="MKNGWEAVIGMEIHAQLATETKIFCACATSFGQEPNSNTCPVCL
                     GLPGALPVLNRRVVDLGARAALALGLEIQATSVFARKNYFYPDLPKGYQISQYDKPFS
                     ADGELTIMTSERDESGRTVEWKPMTIHIERMHLEEDAGKNVHEGLPEVDKYSYIDLNR
                     AGTPLAEIVTAPDFRTSWQAYDYVNHIRRVLQWVGASDADMEKGNLRCEANVSVRKVG
                     DASFNNKVELKNLNSVRFMQKAIEYEIERQIAAHESGEGVNQETRLWDEKNNRTRVMR
                     SKEDAHDYRYFPEPDLQPLMVSSEFIERTKSAMPELPDAMRDRFMREYELSYADASQL
                     VTEKELAEFYERAARASGNPRVSANFILSELLRELNNSGTTAGASPVTAENLAELIKT
                     LDAGKINNNQAKEVLVEMFGSGKNADAVIKEKGFEQISDASAIEKIVDEIIENNQNQV
                     TAYRAGNEKLFGFFVGQVMKASQGKANPKIVNEILKNRL"
BASE COUNT          464 a          410 c          529 g          389 t
ORIGIN      
        1 cagcggctaa ataatttttc gcgtcgcggc agcagaaatt attatcgcgg cggctacgtc
       61 gagccgttga ttggcggcgg cgcttatgcg gagagcgatt ccgaatcgcg cctgagctat
      121 ctcgaacaac agcgcgccgg attaatcgcc gaatggcagg cgctcgaaga agaagcccgc
      181 cgcgccggcg tgagaataga ttaagcgata gaaattttgc aactaaataa aacgcggtta
      241 gaattatcta aatccgcgtt ttatttttta ttatcgaaaa aattatgaaa aatggttggg
      301 aagcagtgat tggaatggag attcacgcgc agctggcgac ggagacgaag attttctgcg
      361 cgtgtgcgac gagcttcggg caggaaccga attcgaatac ttgtccggtt tgtctgggac
      421 ttccgggagc cttgccggtt ttaaaccggc gcgtggtcga tttgggcgcg cgggcggcgc
      481 tggcgctggg gctggagatt caggcgactt ccgtttttgc ccgcaagaat tatttttatc
      541 cggatttgcc gaaaggttat cagatttcgc agtatgacaa gcctttttcg gcggacggcg
      601 agctgacgat tatgacttcc gagcgcgacg aatccggaag aaccgtcgag tggaagccga
      661 tgacgattca tatcgagcgg atgcatctgg aagaagacgc cgggaaaaat gtgcacgaag
      721 gcttgccgga agtcgataaa tattcttaca tcgatttgaa ccgcgccgga acgccgctgg
      781 cggaaatcgt gaccgcgccg gattttcgca cctcctggca ggcgtatgat tacgtcaatc
      841 atatccgccg cgtgctgcaa tgggtcggcg cttcggacgc cgatatggag aagggaaacc
      901 tgcgctgcga agcaaacgtc tcggtgcgca aggtcggcga cgcctctttt aacaacaagg
      961 tcgagctgaa aaatttaaac tcggtgcgct ttatgcaaaa ggctatcgag tatgagattg
     1021 agcgccagat tgccgcgcac gaatcgggcg agggcgtcaa tcaggagacg cgtctgtggg
     1081 acgagaaaaa taaccggacg cgcgtgatgc gctcgaaaga agacgcgcac gattatcgct
     1141 attttcccga gccggatttg cagcctttga tggtttcgag cgaatttatc gagcggacaa
     1201 aatcggcaat gccggagctg cccgacgcga tgcgcgatcg ttttatgcgc gaatacgagc
     1261 tttcttacgc ggacgcttcg cagctggtga cggaaaagga gctggcggaa ttttatgaac
     1321 gggcggcgcg cgcctcaggc aacccgcgcg tgtcggcaaa cttcatattg tcagagcttt
     1381 tgcgagaact gaataattcc ggcacgaccg ccggcgcgtc gcccgtcacg gcggaaaatc
     1441 tggcggaatt aatcaaaact ctggacgccg gaaaaattaa caataatcag gcgaaggaag
     1501 ttctggtcga gatgttcgga agcggaaaaa acgccgacgc cgtcattaaa gaaaaaggct
     1561 tcgagcagat ttccgacgct tccgcaatcg aaaaaattgt tgacgaaatt atcgaaaaca
     1621 atcaaaatca ggtaacggct tatcgcgcgg gcaacgagaa gcttttcggt ttctttgtcg
     1681 ggcaggtgat gaaggcttcg cagggcaaag cgaatccgaa aatcgtcaat gaaattctaa
     1741 aaaacaggct ttaaattctt ttgagtgaga ggaagggtat gaaaacggcg tt
//
LOCUS       DASWNE010000750         7754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_556159, whole genome shotgun sequence.
ACCESSION   DASWNE010000750 DASWNE010000000
VERSION     DASWNE010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7754
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556159"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2366)
                     /gene="gyrA"
                     /locus_tag="VGB68_14645"
     CDS             complement(<1..2366)
                     /gene="gyrA"
                     /locus_tag="VGB68_14645"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEY0050527.1"
                     /translation="METILERNQVNIEDEMRRSYLDYAMSVIIGRALPDVRDGLKPVH
                     RRVLWAMHELGNTHNKPYKKSARVVGDVIGKYHPHGDTAVYDAIVRLAQDFSMRYTLV
                     DGQGNFGSIDGDYAAAMRYTEVRMQKLTSEIMADIEKETVDFQPNYDESLAEPKVLPT
                     RVPTLLINGSEGIAVGMATKIPPHNLTEIVGATIELVKNPDITIDKLIELVPGPDFPT
                     GGFIYGREEIHKAYREGRGILQIRAKAGIDRVGRGEREKDAIVVTEIPYQVNKARLIE
                     KIAELVHEKRLDGISELRDESNREGMRIVIELKRDAVPQVVLNKLYKLTPMQSSFGII
                     NLAIIDGQPRVMNLKQILEAFVEFRREVVRRRTEYELKKARARAHILEGLHKAIDALD
                     YIIPLIRNSRSVDEAKAWLTGNYATLMEVKKWNGAPADKTLAGFLQDLQKVVGRLRFS
                     EIQAQAILDLQLRRLSALERQKIIDELEAILRFISELEAILANEQLLRQVIINELQEV
                     KKNFGDERRTEIIDAGIELKIEDLIPDEEVAITVTKAGYIKRTPVAIYSKQGRGGKGR
                     LGAKAKNEDFVEHLFTASTHNFLMIFTDDGQVYKMKVHEIPEADPSARGKALVNLIQI
                     SSERKLVAVMPVRDFSEEIYLTMVTKLGVIKKTLLSEYQNIRVNGINAINIDDGDELL
                     NVIRTDGKQQIFVATHDGMAVKFNETDVRPMGRVARGVRGVNLRKGDFAVSVCAVSPE
                     GAEKILSISEQGFGKQTQVMNYRLTKRGGIGVINMKTTDKTGKVVAAFP"
     gene            2606..3370
                     /locus_tag="VGB68_14650"
     CDS             2606..3370
                     /locus_tag="VGB68_14650"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY0050528.1"
                     /translation="MPSTTTAEKDERRMQRIGLSLPIRVEYKVNNSVSWSEVTRLRDI
                     STFGAGFMLPRPVKRGRLILMTIPLPRQLRCYDFMEPQYRVYGIVRNCIPHRKNAATP
                     ETYSIGTAFIGKYPPVSYIEDPSKLYDIKPYREEGTLWQVSAAEKNPDESNLPKEHRR
                     HSRFQIPVTVMLETLDEDGNVLAGEITVTENISLSGASVFTSLQADVGSFLRVSSEQY
                     NVSIVSIVRGKRVGPDSIPRLHIEFIDRFFPLEGIE"
     gene            3580..4218
                     /locus_tag="VGB68_14655"
     CDS             3580..4218
                     /locus_tag="VGB68_14655"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY0050529.1"
                     /translation="MKESNSNKYPSPVVIEAIRYFGTFISKIFWRIEFHGTENIPQNL
                     ESGLLVVPNHQSYFDPFWVCIPIRRKYRFMAWEKAFEWFLVGDVMRYLGAFPVNLERG
                     SKESLKQSVKTLREGATLMIFPEGAREFSDGKMLEFKNGAIKIAMMANVPILPVTIRG
                     ANKIWAQDMKFPRLGKVEIFYHPVLEIPREADRREQIDKLTDKLEEIIKSKM"
     gene            complement(4368..6137)
                     /locus_tag="VGB68_14660"
     CDS             complement(4368..6137)
                     /locus_tag="VGB68_14660"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0050530.1"
                     /translation="MIKAGEILQGRYRIERQIGAGGMGAVFVATDERFGSTVAIKETL
                     FTDERLLKAFEREARLLNSLRHPALPRVSDHFVDENGQFIVMEYIAGDDLAEMMEQRA
                     GAFALEDVLKWADQLLDALDFLHTQEMPVIHRDIKPQNLKLTPRGQIILLDFGLAKGS
                     VTNAESLTAAKSVFGYSRNYASLEQIQGTGTDPRSDLYSLAATLYHLMTGAPPSDALT
                     RAMSVLNGEKDPLIPAHHINAAIPAGVSEVLNRAMALNANHRPSSAAAMRELLRDGVN
                     ITDSEEFEAFADVSSTKSLFTQKTAVMADESRASSSELKTSILPVADFSKQTETEDET
                     SIKTNVIKNDSNPNKSTTIQPLAEMTQTAGNQKSRFAAAAMVLGVLLVLGGVASALYV
                     FKPEIFHSAEAPNGKRRLNPADAPKNESNDTSIADANSIVAGAATNTNAPSANPPVLT
                     TVTTKKENLPKADEKAASEIPPAAKNNSRDAKPQPDQPEVRVFERKDENIGDDEVKMT
                     ENRIETKDVIIDEKGIVTFKNPKAKPGRVYNVRPLLTPDQMRQLTPEQREKLRKLMEM
                     RRAMPKFEPPPTAPAPQVKPNQE"
     gene            6294..6860
                     /locus_tag="VGB68_14665"
     CDS             6294..6860
                     /locus_tag="VGB68_14665"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0050531.1"
                     /translation="MQNSPDVTQLLIDWSGGDRNALEQLMPLVYRELQLIAHRHLRRE
                     SQNHTLQTTALVHEAYLKLIDQTRINWQNRAQFFGVTAQAMRRILIDRARERLAEKRG
                     SGVERISLDDGAIDVRDERANELIMLDEALEKLAALDPERSRVVELRYFGGLSAEETA
                     EVMNVSLSTVMRHWRVAKAWLYKELANE"
     gene            7068..>7754
                     /locus_tag="VGB68_14670"
     CDS             7068..>7754
                     /locus_tag="VGB68_14670"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0050532.1"
                     /translation="MSETNWNKIVEIFEQAVDIAPAGRAEFLSSACAGDASLRREVEA
                     MLAADEKADDFIETPAAADASLSLFDKNVSPNTQTIGLAGQKIGAYRIEKEIGRGGMG
                     AVFLAERADGEFQRRVAVKIINRHLRNEFMIRRFRQERQILAALDHPNIARLYDGGTT
                     EDGSPYLIMEYVEGETLLEYCDENRLNVRERLELFGQICAAVEYAHRQSVLHRDIKPS
                     NILVKAGGTAK"
BASE COUNT         1740 a         2084 c         2027 g         1903 t
ORIGIN      
        1 ggaaaagccg ccacgacttt gcccgtcttg tcggtcgttt tcatattgat aacgccgatg
       61 ccgccgcgtt ttgtcaggcg ataattcatc acctgcgtct gtttgccgaa gccctgctcg
      121 gagatggaaa gaattttttc cgcgccttcg ggcgagacgg cgcagaccga tacggcaaaa
      181 tcgcctttgc gaagattgac gccgcgcacg ccgcgcgcga cgcgtcccat cgggcgcacg
      241 tccgtttcgt taaatttgac cgccattccg tcgtgcgtcg ccacgaaaat ctgctgtttg
      301 ccgtccgtgc ggatgacatt cagcagctcg tcgccgtcgt cgatgttgat ggcgttgatg
      361 ccgttgacgc ggatgttttg atactcgctc agcagtgttt tcttaatcac gccgagcttc
      421 gtcaccatcg tcagatagat ttcttcgctg aaatcgcgga cgggcattac cgccacgagc
      481 tttctttcgg aagaaatctg tatgagattg accagcgctt ttccgcgcgc cgacgggtcg
      541 gcttcgggaa tctcgtggac tttcatttta taaacctgcc cgtcgtcggt gaaaatcatc
      601 aggaagttgt gcgtcgaagc cgtaaacaga tgctcgacga aatcctcgtt tttcgccttc
      661 gcgcccagcc gacccttgcc gccgcgtcct tgtttcgaat agattgcgac gggcgttcgt
      721 ttgatataac cggctttcgt gacggttatc gccacttctt cgtcgggaat caaatcttcg
      781 attttcaatt cgattccggc gtcgatgatt tccgtccggc gctcgtcgcc gaagtttttc
      841 ttgacttcct gaagctcgtt gataatgacc tgacgcaaaa gctgttcgtt agcgagaatc
      901 gcttccagct cgctgatgaa tctcaaaatc gcttccagct cgtcgatgat tttctggcgt
      961 tccagagcgg acaaccggcg aagctgcaaa tcgagaatcg cctgcgcttg aatctcggaa
     1021 aaccgcagac gcccgacgac tttctgcaaa tcctgcaaaa atccggcgag cgttttatca
     1081 gccggcgcgc cgttccattt tttgacttcc atcagcgtcg cgtaatttcc ggtcagccac
     1141 gctttcgctt cgtccaccga gcgcgaattg cgaatcagcg gaatgatgta atcgagcgcg
     1201 tcgattgcct tgtgcaagcc ttccaggatg tgcgctctgg ctctggcttt cttcagctcg
     1261 tattcggtgc ggcggcggac gacctcgcgg cgaaactcga cgaaggcttc gagaatctgc
     1321 ttgagattca tcacgcgcgg ctgaccgtcg ataatcgcca gattgatgat gccgaacgag
     1381 gactgcatcg gcgtcaattt ataaagcttg ttcaaaacga cctgcggcac ggcgtcgcgt
     1441 tttaattcaa tcacgatgcg cattccctcg cggttcgatt cgtcgcgcag ctccgaaatg
     1501 ccgtccagtc ttttctcgtg gacgagctcg gcgatttttt caatcaatcg cgccttgttg
     1561 acctgatacg ggatttccgt gacgacgatg gcgtcttttt cgcgctcgcc gcgcccgacg
     1621 cggtcgattc ccgccttggc gcgaatctgc agaatgccgc gaccttcgcg atacgcttta
     1681 tgaatttcct cgcgcccgta gataaagccg ccggtcggaa aatccggtcc cggaaccagc
     1741 tcgataagct tgtcgatcgt aatgtcggga tttttgacca gctcgatggt cgcgccgaca
     1801 atctcggtca gattatgcgg cggaattttc gtcgccatac cgacggcgat gccttcggaa
     1861 ccgtttataa gcagcgtcgg aacgcgcgtc ggcaaaactt tcggctcggc taaagattcg
     1921 tcgtaattcg gctgaaaatc gaccgtttcc ttttcgatgt cagccattat ttccgaagtc
     1981 agcttttgca tacgaacttc ggtataacgc atcgccgccg catagtcgcc gtcaatcgaa
     2041 ccgaagtttc cctgaccgtc gaccagcgtg tagcgcatcg aaaaatcctg cgccaggcgc
     2101 acaatcgcgt cgtagacggc ggtgtcgccg tgcgggtgat atttgccgat aacgtcgccg
     2161 acgacgcgcg ccgatttttt atacggcttg ttgtgcgtgt tgccgagctc gtgcatcgcc
     2221 cacaaaacgc ggcggtgcac cggctttaag ccgtcgcgca cgtccggcaa agcgcgcccg
     2281 atgatgaccg acatcgcgta gtcgagatac gagcgccgca tctcgtcttc gatgttgact
     2341 tgattacgtt ctaaaattgt ttccatttaa atacctttcc aagtaatttc ctcttgattt
     2401 tctatgtgta tgtgagtttg gcaaaaggcg tgatacacaa ataaaagctt gtcatttatt
     2461 cacttttaat gtatattatt ttacaggaat ttgcctcgtt tggcaacctg cccgattggt
     2521 tcaaaaaaga cttctaaccc gttctcccgt caaaatttaa agtcttcaat ttcgtcaaaa
     2581 tctttaacaa aaccagaggt tcaatatgcc gtcgacaacc acagccgaaa aagacgagcg
     2641 ccgaatgcaa agaatcggac tttctctacc gattcgcgtc gaatacaaag tcaacaattc
     2701 agtttcgtgg tcggaagtaa cgcgcctgcg cgacatttcc accttcggcg cgggctttat
     2761 gctgccgcgc ccggtcaagc gcgggcgttt gattctgatg acgattccgc tgccgcgcca
     2821 gcttcgctgc tatgatttta tggagccgca ataccgcgtt tacgggatag tcagaaactg
     2881 catcccgcac agaaaaaacg ccgcgacgcc cgaaacttac tctatcggaa cggcttttat
     2941 cggcaaatat ccgcccgtca gctatatcga agacccttca aagctctacg acatcaagcc
     3001 ttatcgcgaa gaaggcacgc tctggcaggt gtcggctgcc gaaaaaaatc ccgatgaaag
     3061 caatctgccg aaagagcatc gccgtcactc gcgctttcag attccggtta ccgttatgct
     3121 ggagacgctg gacgaagacg gaaacgtcct ggcgggcgaa ataaccgtca ccgaaaacat
     3181 cagcttgagc ggcgcgtcgg tttttaccag cctgcaggcg gatgtcggct cgtttctgcg
     3241 cgtaagcagc gagcaataca acgtttcgat agtttccatc gtgcgcggca agcgcgtcgg
     3301 accggacagc attccgcgcc tgcacatcga gtttatcgac cgtttctttc cgctcgaagg
     3361 aatcgagtaa atccgccgcc cgaaaaaacc ggaaacgcgc gaaaaaaggc gtcccgcgtc
     3421 tttaacaatt ttcttatcgt cgccgacgat tattataaat ttctcattct ccgcacgccg
     3481 acggataccc ggaattcgtc aatgcgggtt ttcccgttga acgaatcgag gtaaattagt
     3541 tattcttgac ggtgtaaaat ttcttttctt taattgcgtg tgaaagaatc aaattcgaac
     3601 aaatatccat cgccggtggt tatcgaagcc attcgctatt tcgggacttt tatcagcaaa
     3661 attttctggc gaatcgagtt tcacggcacg gaaaacatcc cgcaaaatct ggaaagcggc
     3721 ttgctcgtcg tgccgaatca ccagagttat ttcgacccgt tctgggtttg cattcccatc
     3781 cggcgcaaat atcgctttat ggcttgggaa aaagcgtttg aatggtttct ggtcggcgac
     3841 gtgatgcgtt atctgggcgc ttttccggtc aatctggaac gcggcagcaa agaatcttta
     3901 aaacaatcgg tcaaaaccct gcgcgagggc gcgacgctga tgatttttcc cgaaggcgcg
     3961 cgcgaatttt ccgacggcaa aatgctcgaa ttcaaaaacg gcgcgataaa gattgcgatg
     4021 atggcaaatg tgccgattct gccggtcacg attcgcggcg caaacaaaat ctgggcgcag
     4081 gatatgaagt ttccgcgcct cggcaaggtc gaaatctttt atcacccggt attggaaatc
     4141 ccgcgcgaag cagaccgccg cgaacagatt gataaattaa ccgataagct ggaagaaatt
     4201 attaaaagca agatgtaaac ccggcggaag ccggaagatg gaaaaccgga aggtagaagt
     4261 ccggagatag aagaccacaa atcggcggtc ggaaagtcgg ttgaaataga attgagttct
     4321 caattttcat tcaaccagcc gccgactgcc gatttttggt ctgccgtcta ttcctgattc
     4381 ggttttacct gcggcgcggg cgctgtcgga ggaggctcaa atttcggcat cgcgcggcgc
     4441 atttccatta gttttctaag tttttctctt tgttcgggcg tcaactgccg catttggtcc
     4501 ggcgtcagga gcggtctgac attatagact ctgccgggtt tcgctttcgg atttttaaac
     4561 gtgacgatgc ctttctcgtc gatgattaca tccttggttt caatcctgtt ttcggtcatt
     4621 ttcacttcat cgtcgcctat attttcatct tttcgctcaa agacgcggac ttccggctga
     4681 tccggctgcg gcttggcgtc tctggaatta tttttggcgg cgggcggaat ttcggacgca
     4741 gctttttcat cggctttcgg caaattttct ttcttcgtcg tcacggtcgt taagaccgga
     4801 ggattagcgc tcggagcgtt tgtatttgtc gccgcaccgg caacaatcga gttggcgtcg
     4861 gcgatgctcg tgtcgttcga ttcatttttc ggcgcgtctg ccggatttaa acgccgtttg
     4921 ccgttcggag cttcagccga gtggaaaatt tccggcttga aaacgtataa agccgaggcg
     4981 acgccgccga gcacgagcaa tacgccgagc accatcgccg ccgcggcgaa acggcttttt
     5041 tgattgccgg cggtctgcgt catttcggcg agcggctgaa tcgtggtcga tttgttcggg
     5101 tttgaatcgt ttttaatgac gttcgttttg attgaagttt catcttccgt ctcggtttgt
     5161 ttggagaaat cggcgaccgg caaaatcgac gtcttgagtt cggaagacga agcgcgcgat
     5221 tcgtcagcca tcaccgccgt tttctgcgta aaaaggcttt tggttgaaga tacgtcggca
     5281 aaagcttcga attcttcgga atcggtaata tttacgccgt cgcgcaaaag ctcgcgcatc
     5341 gccgcggcag acgacgggcg atgattggcg tttaaagcca tcgcgcgatt taaaacttcg
     5401 gaaaccccgg cgggaatcgc ggcattgatg tgatgcgccg gaatcagagg gtctttttcg
     5461 ccgtttaaaa ccgacatcgc gcgcgtcagg gcgtcggacg gcggcgcgcc ggtcatcaag
     5521 tgataaagcg tggcggctaa agaatataaa tcgctgcgcg ggtcggttcc cgttccctga
     5581 atctgttcga gcgaagcgta attgcgcgaa taaccgaata cgctcttcgc ggcggtcagc
     5641 gattcggcat tcgtcacgct tcctttcgcc agaccgaaat cgagcagaat aatctgcccg
     5701 cgcggcgtca atttcagatt ttgcggcttg atgtcgcggt gaatcacggg catttcctgc
     5761 gtgtgcagaa aatcgagcgc gtcgagcaat tggtcagccc acttcaaaac gtcttcgagc
     5821 gcaaacgcgc cggcgcgttg ttccatcatc tccgccaaat cgtcgcccgc gatgtattcc
     5881 atcacgataa actgaccgtt ttcgtcgacg aaatggtcgc tgacgcgcgg cagcgccgga
     5941 tgccgcaggc tgttcagaag ccgcgcctcg cgctcgaagg ctttgagcag tctttcgtcg
     6001 gtaaaaagcg tttcctttat cgccaccgtg ctgccgaaac gctcgtccgt ggcgacgaaa
     6061 accgcgccca ttccgcccgc gccgatttgt ctctcgatgc gataacgtcc ctgtaaaatt
     6121 tctccggctt ttatcatttt tatagctccc gaaaatctga cgccattcta ctacaaatcg
     6181 tctcaaaaag ttattgcgaa atatcgctgg caaaaggttc tcaaaacccg ataaatcatt
     6241 tacgcgcgcg ggcaaaaata gtttatattg ttcaggtcga accgaaaccg aaaatgcaaa
     6301 attctcccga tgtcacgcaa ttattgattg attggagcgg cggcgaccga aatgctttgg
     6361 aacaattgat gccgttggtt tacagagagt tgcagctaat cgcgcatcgc catctgcgcc
     6421 gcgaatcgca aaatcacact ctgcagacga ccgcgctcgt tcacgaagcc tatctgaagc
     6481 tgattgacca gacgcgcatc aactggcaga accgcgccca gtttttcggc gtcacggcgc
     6541 aggcgatgcg gcggatttta atcgaccgcg cccgcgagcg tctggcggaa aaacgcggca
     6601 gcggcgtcga gcggatttcg ctcgacgacg gcgcaatcga cgtccgcgac gaacgcgcca
     6661 acgagctgat aatgctcgac gaagccctcg aaaagctcgc cgcgctcgac ccggaacgca
     6721 gccgcgtcgt cgaattaaga tatttcggcg gcttgtcggc ggaagaaacg gcggaagtga
     6781 tgaacgtcag cctctcgacc gtaatgcgcc actggcgcgt ggcgaaagcg tggctttata
     6841 aggaattagc gaacgaataa attcaggatt aggatttaag attcaggatt caggattcag
     6901 gattcagaat tacggatttc gaaatacatt cttcttatct cggcaacatc ggcagaggcg
     6961 gaaatcaatt tttcttttgg ctttctttgc gtgaaatcat tattgattgt aatcctgaat
     7021 cttgaatcct gaatcctgaa tcctgaatcc tgaatcctaa attctctatg tctgaaacaa
     7081 actggaacaa aatcgtcgaa atctttgagc aagccgtcga catcgcgccc gccggacgcg
     7141 ccgaattttt aagctccgcg tgcgcgggcg acgcgtcgct gcgccgcgaa gtcgaagcga
     7201 tgctggcggc ggacgaaaaa gccgacgatt ttatcgaaac gcccgccgcc gctgacgctt
     7261 cgctttcgct tttcgacaaa aatgtcagcc cgaacacgca aacaatcggg cttgccggac
     7321 agaaaatcgg cgcgtatcga atcgaaaagg aaatcgggcg cggcgggatg ggcgcggtct
     7381 ttttagccga gcgcgccgac ggcgagtttc agcgacgcgt cgccgtgaaa atcatcaatc
     7441 gccatttgcg aaacgaattt atgattcgcc gtttccggca ggagcggcag attctggcgg
     7501 cgctcgacca cccgaatatc gcgcggcttt acgacggcgg cacgaccgaa gacggctcgc
     7561 cgtatttgat aatggaatac gtcgaaggcg aaacgctgct cgaatactgc gacgaaaacc
     7621 gcctgaacgt cagagagcgc ctcgagctgt tcggacaaat ctgcgcggcg gtcgaatacg
     7681 cgcatcgcca gagcgttctg catcgcgaca tcaagccttc aaacattctg gttaaagcgg
     7741 gcggcacggc gaaa
//
LOCUS       DASWNE010000751         3083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_376517, whole genome shotgun sequence.
ACCESSION   DASWNE010000751 DASWNE010000000
VERSION     DASWNE010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376517"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            535..2490
                     /locus_tag="VGB68_14675"
     CDS             535..2490
                     /locus_tag="VGB68_14675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0050533.1"
                     /translation="MNKRLDSQSSVAYENVFAFYGLLSRDGFLLELKGKIFEENEAMI
                     EPELLVGHKFSETVFWQSASYVAEKLQAGIDEASKGRTTVSELEFRITAQQIQIVDLH
                     LVPQTDESGETAQIYFCAYDVTPRIREIEFYKERSEHFLYAAENSDIGLWFWDFAKGE
                     IFSTPKCNEFFGLAPNEIITPSYFIDAVHPEDRERVAAELQASQNSDKIYESEYRIIC
                     PDGSIQWVSARGKTYFDKDSFPQSMMGIARRITDRKLESEELKKIYDLERKARDEAEE
                     ANRAKDYFLAIVSHELRSPLNSILGWAKILLTKQVDDATRKNALETIERSAKSQAKLI
                     EDLVDMGRVASGKLRLELRPVNLFDIVNNIYNQQKPLAESKKIQLEFLHNAPRAEVFG
                     DAIRLQQVFTNLLNNALKFTPEGGKIKIDMEVTSNKVEVNIIDSGQGIAPEFLPNIFR
                     QFAQGDERTSSDKSGLGLGLSIVKTLVEKHNGFVKAQSQGIGHGSTFTVAFPLHVAAN
                     KEAQETKAERSAKELKLNNVSILLVEDDQDSRDVLALYLEQCGANITGAESAAEAMKY
                     LSNNHRFDVIISDLAMPVEDGYTFLKRVRALPEEKGGKIPAMALSAFTTVENKEKAFA
                     SGFQNYHTKPFEPDLLIKEIAALVNKK"
     gene            complement(2769..>3083)
                     /locus_tag="VGB68_14680"
     CDS             complement(2769..>3083)
                     /locus_tag="VGB68_14680"
                     /inference="COORDINATES: protein motif:HMM:NF023459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbK domain-containing protein"
                     /protein_id="HEY0050534.1"
                     /translation="AETNFNDELLTVKLRYKEPKESQSKLLTIGLSDRGNSIENASDN
                     LKFATAVAQFGLLLRNSRYKGSANFNNVLSLSQRSLGDDLKNYRSEFVDLIGKAKRLS
                     GN"
BASE COUNT          817 a          713 c          746 g          807 t
ORIGIN      
        1 gaaagaaaga cggtcgagac ggttcggagt tttaatcttc tggctaaagt tacacgctaa
       61 ctttcagcga atttaaagct tctttcggtt gagcgaatgc tcagccggat gcggctgctg
      121 agagtatatt ttttgtataa aatctatatt gtgacactgt tttccaatcg acgcgcggtc
      181 tgccgatgcc ggttcgggtt ccggctttcg ccgcttatgt tttcagcacg atagtgattt
      241 tacagactat agacatcagc gcctgaatac gggcgttcaa ttgattaaaa gaactttagg
      301 aagagacgag taaaaatgga aacgagcctt aattttcacc gctgttgttt tgattttttg
      361 gcgggcgttt ctgccggttt gtgagcttgt tttatcaaga taagcgcggt catttatttt
      421 ttgttcggtc ttattatttt taaagtccag tgttgatata tgtccggttt tgcttctaag
      481 aaaaattttt cgtcgtttat ttttgctgga aataaaggtc actccccgga aaggatgaat
      541 aaaagattgg attctcaatc ttcagttgct tacgaaaacg ttttcgcctt ttacggtttg
      601 ttaagccgcg atggttttct gctcgaactc aaaggaaaaa tctttgagga aaacgaggcg
      661 atgattgagc cggaactgct ggtcggacat aaattctccg aaacggtttt ctggcaatcc
      721 gcgtcgtatg tcgccgaaaa gctgcaagcc ggcatcgacg aagcgtcgaa aggcaggacg
      781 accgtctccg agcttgagtt cagaatcacg gcgcagcaaa ttcagattgt cgatttgcat
      841 ctcgttccgc agacagacga atccggcgaa accgcccaaa tttatttttg cgcctacgac
      901 gtcacgccgc gcatcaggga aatcgaattt tacaaagagc gcagcgaaca ttttctatac
      961 gccgccgaaa attccgacat aggcttgtgg ttctgggatt ttgccaaagg cgaaattttt
     1021 tccacgccga aatgcaacga gtttttcggg cttgcgccga atgagattat cacgccgagt
     1081 tattttatcg acgccgttca cccggaagac cgcgagcgcg tcgccgccga gcttcaggca
     1141 tcgcagaaca gcgataaaat ttacgagtcc gaatatcgca taatttgtcc ggacggcagc
     1201 attcaatggg tttcggcacg cggaaaaacg tatttcgaca aggatagctt tccgcagagt
     1261 atgatgggca tcgcgcgccg cataaccgac cgaaaactgg aaagcgaaga gcttaaaaaa
     1321 atctacgacc tcgaacgaaa ggcgcgcgac gaagccgaag aagccaaccg cgccaaggat
     1381 tattttctgg cgattgtctc gcacgaattg cgctcgccgc tgaactcgat tctcggctgg
     1441 gcaaaaatcc ttctgaccaa acaggttgac gacgcgaccc gaaaaaatgc gctcgaaacc
     1501 atcgagcgga gcgcgaaatc gcaggcgaaa ttaatcgagg atttggtcga tatgggacgc
     1561 gtcgcttccg gcaagctgcg gctcgaactg cgcccggtca atttattcga tattgtcaac
     1621 aacatctaca accagcaaaa accgctcgcc gaatcgaaga agattcagct cgaatttctt
     1681 cacaatgcgc cgcgcgccga ggttttcggc gacgctatcc gtttgcagca ggtttttacc
     1741 aatcttttaa acaacgctct gaaatttacg cccgaaggcg ggaaaataaa aatcgatatg
     1801 gaagtgactt ccaataaagt cgaagtcaac atcatcgaca gcggtcaggg catcgctccg
     1861 gaatttcttc cgaatatttt ccggcaattc gcgcagggag acgagcgcac gtcgagcgat
     1921 aaaagcgggc tcggcttggg tctttcaatc gtcaagacgc tggttgaaaa gcacaacgga
     1981 tttgtcaaag ctcagagtca gggcatcgga cacggttcga cctttaccgt cgcgtttccg
     2041 cttcacgtgg ctgccaacaa agaagcgcag gaaacaaaag cagagcgcag cgccaaagag
     2101 ttgaaactga acaacgtcag catcctgctg gtcgaagacg accaggattc gcgcgacgtt
     2161 ttggcgcttt atctcgaaca atgcggcgcg aatattacgg gcgcggaatc cgccgcggaa
     2221 gcgatgaaat atctttccaa taaccaccgc ttcgacgtta ttatttccga tttggcgatg
     2281 ccggtcgaag acggttacac gtttttgaag agggttcgcg ctctgcccga agaaaaaggc
     2341 ggaaagattc ccgcgatggc tttgagcgcg tttacgaccg tcgaaaataa agaaaaggct
     2401 ttcgcttcgg gctttcagaa ttatcacaca aaacctttcg agccggattt gctgattaag
     2461 gaaatcgccg ctctggtcaa taaaaaataa agaaaaagca aggattttta ttacggaaac
     2521 atagacattt tcgcgaaata tcaaggggag aaacatgatt atcaaagatg aaaaagatga
     2581 tacttactca tcagcctgca acactttgtg cctgttaatt ttttcgcttt gtttcgttat
     2641 tttacttttt ttattgcctt gaaatcaggg cgaaaaatgc gaatgtttac gagcgtgtcc
     2701 aatgcttaat attgcggcac gctcgtaaac attcgcattt ctgcctttgt cttttatttt
     2761 aattcgctct aatttccgga aagtcgtttt gcctttccaa tcaaatcgac aaactcgctg
     2821 cggtaatttt ttaaatcgtc gccgagcgat ctttgcgaca ggctcaaaac gttgttgaaa
     2881 tttgccgagc ctttgtagcg cgagttgcgc aaaagcaagc cgaactgcgc caccgccgtt
     2941 gcaaatttca gattgtccga ggcgttttca atcgagttgc ctctgtccga caagcctatc
     3001 gtcagtaatt tactttgcga ttccttcggt tctttgtaac gcagcttaac ggtcagaagc
     3061 tcgtcgttga aattcgtctc ggc
//
LOCUS       DASWNE010000752         3289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_694815, whole genome shotgun sequence.
ACCESSION   DASWNE010000752 DASWNE010000000
VERSION     DASWNE010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694815"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            531..1499
                     /locus_tag="VGB68_14685"
     CDS             531..1499
                     /locus_tag="VGB68_14685"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0050535.1"
                     /translation="MKNFLTISGKFGVLFVCILFLAATVSAQLALREALDTDNDGKAD
                     YTVFRPSSNIWYTLKSTGGFTAQQFGVSNTDYPTPGDFDGDNRGDVAVWRDTDGYWYR
                     LNSSNNTFSAAQFGTTGDEPIARDFDGDGKTDIAVVRRSNGQMFWYSIDSATGAFRGS
                     QFGLSTDFTAPGDYDGDGKFDLAVQRPGSTPTSQGIFYIINSQNGSLRVAPYGFSSDL
                     VVPGDYDGDGKTDLAVVREGSTPTSNLFWFINLSSTNQVAVHSFGITGTDLNAQADYD
                     GDGKTDIAVWRDTNGYFYSLRSSDGGFVGVQWGTGNDFPVASYDTH"
     gene            1601..2623
                     /locus_tag="VGB68_14690"
     CDS             1601..2623
                     /locus_tag="VGB68_14690"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY0050536.1"
                     /translation="MSNIADTIRQEFSRKKIVIVGDLVADQFLSGKIARISREAPVFI
                     LEHEQTETLAGGAANAAVNVAGLGGNACLVGVIGKDVSGEALLESLQKSNVNCTFIAA
                     SEKVQTTTKVRVLASQHYAARQQVIRIDYENKENIDAELIEKLKENLLVAAENADAII
                     ISDYNYGVTTAEIAALASRIAKDKKIPLLVDSRYRLQDFTGGTSATPNQEEAEQILGE
                     KFTEIAPLADACVRLREKLDYESLLVTCGNKGMFLIEKDRKPLYLEAVGSKEPVDVTG
                     AGDTVIAAYALALASGLSFGESANLANHAGGIVVMKKGTASVSVDELLSSIAKDERIE
                     HKTQTL"
     gene            2636..3127
                     /locus_tag="VGB68_14695"
     CDS             2636..3127
                     /locus_tag="VGB68_14695"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEY0050537.1"
                     /translation="MSSSVNSFAPILERNRLMARVAIERRGGARIVLANGCFDLFHVG
                     HIRYLAGAKALGEILIVGINSDEQVRKLKGENRPFMPETERAEIVSALKFVDYVTIFP
                     EATVTELIRAVRPDFHAKGTDYTTDTVPEREIVREYGGRVAIVGDPKDHSSTDLIGKV
                     AGS"
BASE COUNT          818 a          832 c          881 g          758 t
ORIGIN      
        1 gggcgcacgg atttcgccgt ctggcgaccg aatcagaacc cgaacgaatc gggaatcttt
       61 tacgtgcagc gcagcacagc cggattttcg gctttcggct ggggaaattc cggtatgaaa
      121 attcccgcta attcgatgca gagccagtaa ttgcagccgg aaatcggaaa atgaatttta
      181 agaaaatgta tttttctgcg cccgagccgc actgtataaa tagcggaaac gacgaaatag
      241 cgaaaatggc ggaaaatcgg cagggaaaaa acttttaacc gttgccggtc gctgccgcca
      301 attattttca gacggataca aacttttttc ctgaaccgcg cgtctaactt ccgtatgtcg
      361 gaaaaatctc gggaatagtt atattaatcc ggaaatcagc atttttagcc ggtattatct
      421 tgcaatcgca aggttttcgg tagtatattt atgaatccga gtctttatgg cgcggacgcc
      481 ccgaccccca ttggagaatt tctaaaagaa gtaagagaag gattaacgca atgaaaaact
      541 ttttgaccat tagcggcaaa ttcggtgttt tgtttgtctg cattttattt ttagcggcga
      601 ccgtctccgc tcagctcgcg ctgcgcgaag cactggatac cgacaatgac ggcaaagccg
      661 attacacggt ttttcgtccg agcagcaaca tctggtatac gctgaaaagc accggcggat
      721 ttaccgctca gcagttcggc gtctcgaata ccgactatcc gacgcccggc gacttcgacg
      781 gcgataaccg cggcgatgtg gcggtttggc gcgacacgga cggctactgg tatcggctga
      841 acagctcaaa caatactttc tctgccgcgc agttcgggac gaccggcgac gagccgattg
      901 cgagagattt cgacggcgac ggcaaaaccg atatcgcggt ggtgcgtcgt tccaacggtc
      961 agatgttctg gtatagcatc gacagcgcga ccggcgcgtt tcgcggctct cagtttggtc
     1021 tttcgaccga ttttaccgcg cccggcgatt atgacggcga cggtaaattc gacctcgccg
     1081 tgcagcgtcc cggctcgacg ccgaccagcc agggaatttt ttatatcatc aacagccaga
     1141 acggcagcct gcgcgttgcg ccttacgggt tcagcagcga tttggtcgtg ccgggcgatt
     1201 atgacggcga cggcaaaacc gatttggcgg tcgttcgcga aggctcgacg ccgacttcca
     1261 atcttttctg gtttatcaac ctgagctcga ccaatcaggt cgccgttcat tcgttcggaa
     1321 ttaccggcac ggatttaaat gcccaggctg attatgacgg cgacggtaaa accgacatcg
     1381 ccgtctggcg tgatacgaac ggctatttct acagcctgcg cagctcggac ggcggattcg
     1441 tcggcgttca atggggaacc ggcaacgact ttccggtcgc cagctacgat acgcactaat
     1501 ttgcgcgtca attggtcaaa gcaaaaggtt gaaggctgaa aacggtcttc aacctttttg
     1561 tttatctttt tgagttgtag taatttattt cttagctcga atgtcaaaca tcgcagacac
     1621 tattcgtcag gagttttccc gaaaaaaaat tgttatcgtc ggagatttgg tcgccgacca
     1681 atttctttcc ggcaaaattg cgcgtatctc gcgcgaagcg cccgttttca ttctggaaca
     1741 cgaacaaacc gaaactctgg cgggcggcgc ggcaaatgcg gcggtcaacg tcgccggatt
     1801 gggcggcaac gcctgtctgg tcggagtaat cggaaaagat gtgagcggcg aagcgctgct
     1861 tgaaagcctg caaaaatcaa atgtcaactg cacttttatc gccgcttccg aaaaagttca
     1921 aacgacgacg aaggttcggg tgctcgccag ccagcattac gccgcgcgcc agcaggtcat
     1981 tcgcatagac tacgaaaaca aggaaaatat cgacgcggaa ttaatcgaaa aactaaagga
     2041 aaatcttctc gtggcagccg aaaacgccga cgcgattatt atttccgatt acaattacgg
     2101 cgtgacgacc gcggaaatcg cggcgctcgc cagccggata gcgaaggata aaaaaattcc
     2161 tctgctcgtc gattcgcgtt atcgtttgca ggattttacc ggcggaactt ccgccacgcc
     2221 gaatcaggaa gaagccgaac aaatcctcgg cgaaaagttt acggaaatcg cgccgctggc
     2281 tgacgcctgc gttcgcctgc gcgaaaagct cgattacgag tccttgctcg tcacctgcgg
     2341 caataaagga atgtttctga ttgagaaaga ccggaaaccg ctctatctgg aagccgtcgg
     2401 ctcgaaagag ccggttgacg tgacgggcgc gggcgatacg gtcatcgccg cttacgcgct
     2461 ggcgctggct tcgggtttga gcttcggcga aagcgcgaat ctggcgaatc acgcgggcgg
     2521 catcgtcgtg atgaaaaaag gcacggcatc ggtcagcgtc gacgaattgc tttcttctat
     2581 cgccaaagat gaaagaatcg agcataaaac tcaaacgctg taaatttaaa tttttatgag
     2641 ttcttctgta aattcatttg cgccgattct ggagcgcaat cgcctgatgg cgcgagtcgc
     2701 catcgaaagg cgaggcggcg cgcgcattgt gctggcaaac ggatgtttcg acctttttca
     2761 cgtcgggcat attcgctatc tggcgggcgc gaaggctctg ggcgagattc tcattgtcgg
     2821 catcaattcc gacgagcagg tcagaaaact gaaaggcgaa aatcgcccgt ttatgccgga
     2881 aacggaacgc gccgaaatcg tttcggcgct gaaattcgtc gattacgtga cgatttttcc
     2941 cgaagcgacc gtgacggaat tgattcgcgc cgtgcgcccg gattttcacg cgaaaggcac
     3001 ggattacacg accgacaccg ttcccgaacg agaaatcgtc cgcgaatacg gcgggcgcgt
     3061 ggcaatcgtc ggcgacccga aagaccattc ttcgaccgat ttgatcggga aagttgcggg
     3121 aagttgacag aaagttgaca gaaagttgca ggaagttgga agagattaaa aacagttcaa
     3181 agtttcaatc ttcgacagtt ctcttaacta tctgcaactt tctgcaactt tctgtcaact
     3241 tcctgcaact tcctgttaac tattatgtgg aaatcgcgca cgaaaaacg
//
LOCUS       DASWNE010000753         2810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_556261, whole genome shotgun sequence.
ACCESSION   DASWNE010000753 DASWNE010000000
VERSION     DASWNE010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556261"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2810)
                     /locus_tag="VGB68_14700"
     CDS             complement(<1..>2810)
                     /locus_tag="VGB68_14700"
                     /inference="COORDINATES: protein motif:HMM:NF019136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEY0050538.1"
                     /translation="FAAASHAQYVPFRFEKFGVDNGLSNQIVTCTLRDATGYLWVGTE
                     NGLNRFDGYGFKHFLPDANNRTGLQDGSITTLFEDSKRNLWVGTKSGGLHLFDRRTET
                     FYVFRHDPKNQNGLSDNRIYRIYEDTDGSLWIGTPGGLDKAVGTGDAPEKVRFTSFRN
                     DPNNPESLSNNRVGAIVRDAAGNLWIGTKQGLNRMISESPARFERFAHDKNKPDSLPD
                     DEVYSLLSDKEGNLWIGLWGGGGLSKINASELSKPQPAFLRFPYDKKTQTGLEFDIVV
                     NLTEDSRGNIWLANGDHERLTYLAQEEKNKPNPRFVSFAGDEGNPFSLGSGAVYSVSE
                     DAQGLLWIAASTGGLNKLDLSKSGFKIYRYNPNNTNPSLLENNAGHAVEDFDGSIWIG
                     SEKGITQIVPGAEAFSQPTYTYYKTGADAKNGLRSQGIETLLLDSKGRLWAGTINGGL
                     KLRQKQGDKFNFISFEKDANNPQSLGNNTVLTLLEDSRGSIWIGTYQGLYKMVEPTAE
                     NPQTTFLAYRSKKGDAGSLSNDIIESLAEGADGSIWAGTVSGLNRLEPASGKITRYAP
                     EAENPKSLNNEFINNLFQSAKGALWIGTRKGLCRFDSATQTFTRLAPETGLADAGISA
                     MAEDAEGFLWVATTNGLFRFDPEKNASRHFGKNDGLPGDIFNRNAAFRGKNGTLYFAG
                     TQGLIAFNPRDIKDNAFTPPVVLTEFFLANQAQPIQAQSILPAHIQFLEKIVLNYTDN
                     IFGIEFSALNYKQPEDNRFAYKLEGLSDDWIETDGKNRRAMFTNLNPGEYVFRVKAAN
                     NDGVWNENGASVRVVVLPPWWKTWWAQTLFYSILIIGLLAVPFLRLSRLKKQRAALEK
                     QVAARTSELSETNQKLEEANRAKSVFLSNMSHELRTPLNAVIGFSQLLGRDSGLTSQQ
                     GETLGVIHRSGEHLLGLINDVL"
BASE COUNT          598 a          763 c          733 g          716 t
ORIGIN      
        1 aaagaacgtc gttgattaaa ccgagcagat gctcgcccga acgatgaatg acgccgagag
       61 tttcgccctg ctgcgacgtc aagcccgaat cgcgccccag caattgcgaa aacccgatga
      121 cggcattgag cggcgttctc aattcgtgac tcatattcga gagaaaaacg cttttcgcgc
      181 ggttggcttc ttccagtttc tggttggttt cgctcagttc ggacgtgcgc gccgcgacct
      241 gtttttccag agccgcgcgc tgctttttca aacgcgaaag acgaaggaaa ggcaccgcca
      301 gcagaccgat tattaaaatc gaataaaaca aagtttgcgc ccaccaggtt ttccaccagg
      361 gcggcaaaac gacgacgcgc acggatgcgc cgttttcgtt ccagacgccg tcgttattgg
      421 cggctttgac gcgaaaaaca tattcgcccg gattcagatt ggtaaacatc gcgcggcggt
      481 ttttgccgtc cgtctcgatc caatcgtccg acaagccttc cagcttataa gcaaagcgat
      541 tgtcttccgg ctgcttgtag ttgagcgcgc tgaactcgat gccgaaaata ttgtccgtgt
      601 aatttaagac gattttctca agaaattgaa tgtgcgccgg aagaatcgac tgcgcctgaa
      661 tcggctgcgc ctgattcgcc agaaaaaatt ccgtcaaaac gacgggcggc gtgaaagcgt
      721 tgtcttttat atcgcgcggg ttaaaggcga ttaagccttg cgttccggca aaatagagcg
      781 ttccgttttt tccgcgaaaa gcggcgttgc gattaaaaat gtcgcccggc aaaccgtcgt
      841 ttttgccgaa atgacggctc gcgttttttt ccgggtcgaa gcgaaacaac ccgttggtgg
      901 tggcgaccca cagaaagcct tcggcgtctt ccgccatcgc gctgatgccc gcgtcggcga
      961 gtccggtttc cggcgctaga cgcgtaaagg tctgcgtcgc cgaatcgaat ctgcacagcc
     1021 ctttccgcgt tccaatccac agagcgcctt ttgcagattg aaataaatta tttataaatt
     1081 cgttgttgag gctcttcgga ttttccgctt cgggcgcgta acgagttatt ttgcccgacg
     1141 ccggttcgag ccggttcaag ccggaaaccg tgccagccca gatgcttccg tccgcgcctt
     1201 cggcgagcga ttcgatgatg tcgttgctta aactgccggc gtcgcctttt tttgaacgat
     1261 acgcgagaaa agtcgtctgc ggattttccg ccgtcggctc gaccattttg tataagccct
     1321 gataagttcc gatccagatg ctgccgcgcg aatcttccag cagcgttaaa accgtgttgt
     1381 ttcccaggct ttgcggatta ttcgcgtctt tttcaaagct tataaagtta aatttatcgc
     1441 cttgcttttg ccgcagtttc aaaccgccgt tgattgtgcc agcccagagc ctgccctttg
     1501 aatcgagcag aagagtttcg atgccctgcg agcgcaatcc gttttttgcg tccgcgccgg
     1561 tcttgtaata agtgtaggtc ggctgggaaa aggcttcggc gccgggaacg atttgcgtaa
     1621 tgcccttttc gctgccgatc cagatgcttc cgtcgaaatc ttcgacggcg tgaccggcgt
     1681 tgttttccag caggctcgga tttgtgttgt tcggattgta gcggtaaatt ttaaatcccg
     1741 atttacttaa atccagcttg ttcaaaccgc ccgtcgaggc ggcaatccat aacaatcctt
     1801 gggcgtcttc ggaaacggaa tagaccgcgc cgctgccgag gctgaacgga tttccctcgt
     1861 caccggcaaa actcacaaaa cgcggattcg gtttattttt ctcttcctga gcgagatacg
     1921 tcaatcgctc gtggtcgccg ttggcaagcc agatgtttcc gcggctgtcc tcggttaaat
     1981 tcaccacgat gtcgaattcg agcccggttt gagttttttt atcataagga aaacgcagga
     2041 atgcgggttg cggttttgat agctccgaag cattgatttt agaaagaccg ccgccgcccc
     2101 acaagcctat ccagagattg ccttccttgt ccgaaagcag gctgtaaacc tcgtcgtcgg
     2161 gcaggctgtc cggtttattt ttgtcgtgcg cgaatcgctc gaagcgggcg ggactttcgg
     2221 aaatcatccg gttcagtccc tgtttcgtgc caatccacaa atttcccgcc gcgtcccgca
     2281 cgattgctcc gacgcggtta ttgcttaggc tttccggatt gttcgggtca tttctaaacg
     2341 aggtaaaacg aaccttttcc ggcgcgtcgc ccgtcccgac ggctttatcc agaccgcccg
     2401 gcgtgccgat ccagaggctg ccgtccgtgt cttcgtaaat tcgatagata cgattatcgc
     2461 tcaagccgtt ttgatttttc gggtcgtgac ggaaaacgta aaacgtttcc gtccggcggt
     2521 cgaaaagatg caagccgccg cttttcgttc cgacccacaa attccgcttg ctgtcttcaa
     2581 acagggtcgt gatggagccg tcctgcaaac ccgtcctgtt attggcatcg ggcagaaaat
     2641 gtttaaagcc gtagccgtca aaacgattca gcccgttttc cgttccgacc cagagatagc
     2701 ccgtcgcgtc gcgcaatgtg caggtcacga tttgattcga aagcccgttg tcgacgccga
     2761 atttttcaaa gcgaaacggc acgtattgag cgtgcgaagc agcggcgaaa
//
LOCUS       DASWNE010000754         1757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_661406, whole genome shotgun sequence.
ACCESSION   DASWNE010000754 DASWNE010000000
VERSION     DASWNE010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661406"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VGB68_14705"
     CDS             complement(<1..440)
                     /locus_tag="VGB68_14705"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY0050539.1"
                     /translation="MRRTSFLLAFAIAALFVFSNSGTQAVNNSFVEKFASFDDKKKQD
                     IDYSGAYSFDKAHSFIGFKVKHMGLIEVPGFFRDFTGTVNYDAKDVSKSSVEFTAKAT
                     SVDTGVAGRDKHLRTADFFDVEKFPDLTFKSTKVEKKGKNLMVTG"
     gene            complement(579..1184)
                     /gene="wrbA"
                     /locus_tag="VGB68_14710"
     CDS             complement(579..1184)
                     /gene="wrbA"
                     /locus_tag="VGB68_14710"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF002999.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HEY0050540.1"
                     /translation="MAKVAIIYYSSTGTTYQLAKAVEEGARAAGAETRLLKVRETAPE
                     AAINSNEKWKNHAEEARDIAEATMDDLKWADAVIFGTPTRYGGATSQLRAFIDQTGGL
                     WASGALVNKIGSSFTSTATSHGGQESTILSLNTIFYHWGAIIVSPSYADPIQFQSGNP
                     YGVSFTSQNGEASPDETALGAARFQGRRVAEVTAQFLKGRE"
     gene            complement(1243..>1757)
                     /locus_tag="VGB68_14715"
     CDS             complement(1243..>1757)
                     /locus_tag="VGB68_14715"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY0050541.1"
                     /translation="KSFDNSRYNDYSFYVMSELQKQLRQAKPFKSKEEELYLNLARTT
                     EALNWKVAELLKSHELTGVQYNVLRILRGAGEEGLICSEISERLITKDSDITRLLERM
                     ENRDLISRRRSAKDRRHVITKITDTGLKLLAELDEPVVSLHRQQLGHLGENLLEQLNA
                     LLVLARSQVN"
BASE COUNT          408 a          462 c          398 g          489 t
ORIGIN      
        1 cccgtcacca ttagattttt gcctttcttt tcgactttcg tgcttttgaa agtcaaatcg
       61 ggaaattttt cgacgtcaaa aaaatcagcc gtgcgcaaat gtttgtcgcg tccggcaacg
      121 cccgtatcga cgctcgtcgc cttcgccgta aactcgaccg aagatttcga cacgtctttc
      181 gcatcgtaat tgaccgtgcc ggtaaaatca cggaaaaagc cgggaacttc aatcaagccc
      241 atatgcttga ctttaaagcc gataaagctg tgcgccttat cgaagctgta agctccgctg
      301 taatcgatgt cctgtttttt cttatcgtcg aaagacgcga atttttcgac aaaagaattg
      361 ttgacggctt gcgtgcccga gtttgaaaaa acaaacagcg cggcaatcgc gaaagccagt
      421 aggaatgaag ttcttctcat ttttaatttt gctccttaat tgaatctttg atgaatgaat
      481 tacgcgataa aaatcgtttg atgcaaattt ataaaaaata gtcgattgat aattgataaa
      541 acttccccga attatcaatt atcaatcggc tatcgaaatt actctctgcc ctttaaaaac
      601 tgcgcggtta cttccgccac gcgcctgccc tgaaaacgag cggcgccgag cgcggtctcg
      661 tccggcgaag cttcgccgtt ttgcgacgtg aacgaaacgc cgtaaggatt gccggactga
      721 aactgaatcg ggtcggcata agacggcgag acgataatcg cgccccagtg ataaaaaatc
      781 gtgttgagcg aaagaatcgt cgattcctgc ccgccgtgcg aggtcgccgt cgaggtaaac
      841 gacgagccga ttttgttgac cagcgcgccg ctcgcccaaa gtccgcccgt ctgatcgata
      901 aacgcccgca gctgcgatgt cgcgccgccg taacgcgtcg gcgttccgaa aattacggcg
      961 tccgcccatt tcaggtcgtc catcgtcgcc tcggctatat cgcgcgcttc ctcggcgtga
     1021 tttttccatt tttcgttgga atttatcgcc gcttccggcg cggtttccct cactttcaga
     1081 agccgcgttt ccgcgcccgc cgcccgcgcg ccttcttcga cggcttttgc cagctgataa
     1141 gtcgttccgg tcgagctgta ataaattatt gccactttcg ccattttgat ttctctcctt
     1201 tttatacaat taataatcgt gataacaact gttttaatag aattaattga cctggctgcg
     1261 cgctaaaacg agcaaagcat taagctgttc caaaagattt tcgccgagat gcccgagctg
     1321 ctgccggtgc aggctgacga ccggctcgtc aagctcggct aaaagcttta agcccgtatc
     1381 ggtgattttc gtaatcacgt ggcggcggtc tttcgcgctt cgtcggcgcg agattaaatc
     1441 gcggttttcc atcctttcga gcagtctcgt gatgtcagag tcttttgtaa tcaggcgctc
     1501 gctgatttcg ctgcaaatca agccttcttc gccggcgccg cgcaaaattc tcaacacatt
     1561 gtactgaacg ccggtcagtt catggctttt gagcagttct gccactttcc agtttaatgc
     1621 ttccgtcgtg cgcgccagat ttaaataaag ctcttcttcc ttgcttttaa acggcttcgc
     1681 ctgcctgagc tgtttttgca attcactcat aacataaaaa gaatagtcgt tataacgact
     1741 attgtcaaat gattttt
//
LOCUS       DASWNE010000755         2642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_164381, whole genome shotgun sequence.
ACCESSION   DASWNE010000755 DASWNE010000000
VERSION     DASWNE010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164381"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VGB68_14720"
     CDS             <1..690
                     /locus_tag="VGB68_14720"
                     /inference="COORDINATES: protein motif:HMM:NF012995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HEY0050542.1"
                     /translation="QKQHLLHVPFENLDIHWRRLIVPDVEKFYGKIVEENRGGFCYEL
                     NGLFYRLLREIGFQSRMISARVAGGENGFGAEYDHLAILTKIDGEEFLVDVGFGSFTA
                     EPLKFTSDLEQQDKNGVFLIRKFDADYFEVLKKDAGAWKSQYIFTPFERDLSEFAGMC
                     RFHQTSNESHFTRGKICSLMTVEGRKTLTDRKFIETGNEEKREFSVDSEAEFNEILER
                     EFHIRPVFGQT"
     gene            775..1809
                     /gene="purM"
                     /locus_tag="VGB68_14725"
     CDS             775..1809
                     /gene="purM"
                     /locus_tag="VGB68_14725"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEY0050543.1"
                     /translation="MTKSISYSDAGVSIDKANEAVSKIREFAKATFNERTLTEIGSFG
                     GMFSAAFPEMSEPVLVASADGVGTKLKVAFLAGVHDTVGQDLVNHCVNDILVQGARPL
                     FFLDYFATGSLEPDVTASVVEGMAKACRENGCVLLGGETAEMPSMYSAGEYDLAGFIV
                     GIVDKSKVIDGKNIKAGDVVLGLPSNGLHTNGYSLARKLLLETGKYSISSVLPEMHST
                     LDEVLLKPHKSYLHVLEKLLDSGKIKGLAHITGGGLLENIPRILPENVSVEIKRGTWE
                     ELPIFGLMQKLGNVEEKEMFRTFNMGVGMVVICAAEDSQDLKEHFANAGEACDEIGRV
                     SDGSKQVSII"
     gene            1813..2418
                     /gene="purN"
                     /locus_tag="VGB68_14730"
     CDS             1813..2418
                     /gene="purN"
                     /locus_tag="VGB68_14730"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEY0050544.1"
                     /translation="MKIGILISGRGSNMVAIAEAVQSGRIPGSEITVVLSDKKSAQGL
                     EKAKERGIETIVVARNGRTREEHDAEIVAELKKRNVELVCLAGYMRLLSRDFVGAFPD
                     KIVNIHPSLLPAFPGLDAQRQAIEYGVKVSGCTVHFVDDDLDHGAIILQKTVEVKDED
                     TAETLSARILAHEHALYVEAIKHIAEGKIEIKGRKVFRKSN"
BASE COUNT          689 a          568 c          734 g          651 t
ORIGIN      
        1 cagaaacaac atcttttgca cgtgccgttt gaaaatctcg atattcactg gcggcgattg
       61 atcgttccgg acgtcgaaaa gttttacggg aaaatcgtgg aagaaaacag aggcggtttt
      121 tgttacgagc taaacggttt attttacagg ctgttgcggg aaatcgggtt tcaaagccgg
      181 atgatttccg cgcgggtcgc gggcggcgaa aacggtttcg gcgcggaata tgaccatctg
      241 gcgattctga cgaaaatcga cggtgaagaa tttctggtcg acgtcggttt cggaagcttt
      301 accgccgagc ctttgaaatt tacttcggat ttggaacagc aggacaaaaa cggcgttttt
      361 ctgattagaa aatttgacgc cgattatttt gaagtcctga aaaaagacgc gggcgcttgg
      421 aaaagccaat atatttttac gcctttcgag cgcgatttgt cggaattcgc cggaatgtgc
      481 cgttttcatc aaacctcgaa cgaatcgcat tttacgcgcg gcaaaatttg ctcgctgatg
      541 actgttgaag ggcgcaagac tttaaccgac aggaaattta tcgaaaccgg aaacgaagaa
      601 aagagagagt ttagcgtcga ttcggaagcg gagttcaacg agattttaga gcgtgaattt
      661 cacatcagac cggttttcgg acagacgtaa aacgcttctt ttcgagccgt aatttatccg
      721 tcgccgtttg tggtcaattt ttctccggcg gtttataatc gcgtttttaa agctatgaca
      781 aaatcaattt catattccga cgcgggcgtc tcgattgata aggcgaacga agcggtttca
      841 aaaatcagag aatttgcgaa agcgactttt aacgagcgaa ctttgacgga aatcggcagc
      901 ttcggcggaa tgttttcggc ggctttcccg gaaatgagcg agccggttct ggtcgcttcg
      961 gcggacggcg tcgggacgaa actgaaagtc gcttttctcg cgggcgttca cgatacggtc
     1021 gggcaagacc ttgtaaatca ttgtgttaac gacattttag tgcagggcgc gcgacccttg
     1081 ttttttctcg attattttgc gaccggaagc ctcgagccgg atgtgacggc ttcggtcgtc
     1141 gaaggaatgg cgaaggcttg ccgcgaaaac ggctgcgttc tgctcggcgg cgaaacggcg
     1201 gagatgcctt cgatgtattc ggcgggcgaa tacgatttgg cgggctttat cgtcggcata
     1261 gtcgataaat cgaaagtcat cgacggaaaa aacatcaagg cgggcgatgt cgttctgggc
     1321 ttgccgtcca acggcttgca cacgaacggt tattcgctgg cgcgaaagct tttgctggaa
     1381 acgggtaaat acagtatttc gagcgtttta ccggaaatgc attcgacgct ggacgaagtt
     1441 ttgctcaaac cgcataaaag ttatctgcac gtgctggaaa agctgctgga ttcaggaaag
     1501 ataaaaggct tggcgcacat taccggcggc ggtttgctgg aaaatattcc gcgcattctg
     1561 ccggaaaacg tttcggtaga aataaaacgc ggaacgtggg aagaattgcc gattttcgga
     1621 ttgatgcaaa aactcggaaa cgtcgaagaa aaagaaatgt ttcgcacttt taacatgggc
     1681 gtcggaatgg tcgtgatttg tgcggcggaa gattcgcagg acttgaaaga acattttgcg
     1741 aatgcgggcg aagcctgcga cgaaatcggg cgggtttcag atggaagcaa acaggtttca
     1801 ataatttaaa agatgaaaat cggcatttta atttccgggc gcgggtcgaa tatggtcgct
     1861 attgccgaag ccgttcagag cgggcgcatt cccggttccg agattacggt cgttctgagc
     1921 gataaaaaaa gcgcacaggg cttggaaaag gctaaagagc gcgggataga gacgattgtc
     1981 gtggcgcgaa acgggcgcac gcgcgaagaa cacgatgcgg aaatcgtcgc cgagctgaaa
     2041 aagcgaaatg tcgagctggt ttgtctcgcc ggttatatgc ggcttctgtc gcgggatttc
     2101 gtaggcgcgt ttcccgataa aatcgtcaac attcatccga gcctgctgcc ggcgtttccg
     2161 ggcttggacg cgcagcggca ggcgattgaa tacggcgtga aagtttcagg ctgcacggtt
     2221 cattttgtcg acgacgattt agaccacggc gcgataattt tgcaaaagac ggttgaagta
     2281 aaagatgaag atacggcgga aacgctctcg gcgcgtattc tggcacacga acacgcgctt
     2341 tatgtcgaag cgattaaaca catcgccgaa ggaaaaatcg aaattaaagg gcgaaaagtt
     2401 ttcagaaaat caaactaatt cgagccaaac gttttcgatt atttcgccga ttacggagat
     2461 ttccctttcg tttctcgacg cttttaaaga aaccggaata taaacgcggc gattatcctt
     2521 gctccgacga ttgtaaccgg caaagcctca atttcccttc accgacctgc cgcaagcaag
     2581 ggcgatttca ctctacggtt ttcgaaaagc tgcaattgtc tcgctcggtt tttgacataa
     2641 tc
//
LOCUS       DASWNE010000756         4763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_590448, whole genome shotgun sequence.
ACCESSION   DASWNE010000756 DASWNE010000000
VERSION     DASWNE010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590448"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VGB68_14735"
     CDS             <1..435
                     /locus_tag="VGB68_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050545.1"
                     /translation="GRKTDMKSLKLKQFAISLLAVTAFFVSSISACTCSHHQAKQETV
                     SASCHEHSAKTVQSRDDDLPETIETSISEAGCVCFQSARKAFAKSENVKFEKHAAAIS
                     PLTPPAIVSIPQNARVKLDFTKPFYLSDSFYNFSHGRAPPVL"
     gene            554..2533
                     /locus_tag="VGB68_14740"
     CDS             554..2533
                     /locus_tag="VGB68_14740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050546.1"
                     /translation="MKTLFLSAIFALLLGVNAFAHEPFKLVSSERKTVREADLAALEK
                     NQTVGAIDKTNLTFTAKEIRLVAVTGPEDDMLSYRIQGVRNPNLIVPSGALLRILFVN
                     VDVDMRHDIRFGHVAGDFPIAPEIAGTAGSQKLEKRAGDDALQAEEIVVQAKEDGAFK
                     YFCSIRGHAKGGMWSNILVDVKPGGNLKTATKTEHVHTEDEDRDTDEKKTNDKKPTEH
                     NHDMKTDEMKTDDKKSENMPEMKTDDKKMSGMPHDEKKMSAITDINAPMSRESSGTAW
                     LPDSSPLYGTMKMFKDGSMLMFHGNLFLRYTRAGSRRDFSAGGKGDNARFDAPSMFML
                     MYSKPIDRKSQIGLRAMVSLDPVIERGYGYPLLYQSGETYRGHPLHDRQHPHDFFSEL
                     SASYSYRINEKQSFFFYAGYPGEPALGPPTFMHRVSAMNNADAPLSHHWQDSTHITWG
                     VLTAGYNFGKFKFEASAFKGQEPDENRWNLDAPKLDSFSGRFSFNPNKEWAFQISYGY
                     LKNPEPSEPNLKILRRTTASAIYNKKFGDDKNWANSFVWGQNHGNGERTDAFLFETDY
                     QFRKNSIFGRVERVEKSGHELALDEIDHDKIFWVGAYSLGYVRELYRNKNLNVGLGAQ
                     AGFYQNPNGLNAYYGGTKHQSWQIFLRLRPSAMKH"
     gene            2543..>4763
                     /locus_tag="VGB68_14745"
     CDS             2543..>4763
                     /locus_tag="VGB68_14745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEY0050547.1"
                     /translation="MHEKHLREEMREDKSGTHIDPICGMTVNPATAAGSFKHEGTTYY
                     FCSKGCLQKFINQTSGAPPSGLVQLGRRKEIVRHGEMTAAPQGEFIDPVCGMSVAPET
                     AAGKYDFQGRSFYFCSTGCLHKFKQNPSNFLEKKKEETKAAASEGVEYTCPMHPEIVQ
                     IGAGTCPKCGMALEPKTVSLDDAPDPEYLDMKRRFWISAVLTLPVFLLAMAEMLPGFH
                     SFIAPERALWIQFILATPVVLYCGFPFFVRGAQSVKNLSPNMFTLIAVGTGAAYLYSL
                     AAVFFPGVFPASLHNPHTHLISVYFEAAAVITTLVLLGQVLELRARSQTSSALKELLG
                     LAPKTATALFDDGSEAEISLADVQKNARLRVRANEKIPTDGVILEGDSTIDESMVTGE
                     PIPIEKTKGDRVIGGTINGNRAFVMRVEKVGSETLLAQIVQMVGEAQRSRAPIQRLAD
                     IVSAYFVPAVIVVAIVTFLVWALLGSFTSGFVYAVAVLIIACPCALGLATPMSIMVGT
                     GRGAKAGVLVKKAEALEILEKINTIVVDKTGTLTEGKPRLQAVVSLSGSDENEILRFA
                     ASLEKSSEHPLAEAIVKGALERNVGFAKVSDFQSITGKGLIGKIDGKEIKIGNEKFLV
                     QENFKQDEQDKNPANPVRPVNSVSIRDKANELRAQGQTVMFVSVNGNLAGLVGVADTI
                     KESAKEAIAGLRRQKIEIVMMTGDNQATAEVVARQLGIDKVFGDVLPDEKASKVKELQ
                     "
BASE COUNT         1238 a         1179 c         1288 g         1058 t
ORIGIN      
        1 gggcggaaaa ctgatatgaa gagtttgaaa ttaaaacaat ttgccatcag ccttctggcg
       61 gtcacggctt tctttgtatc ctcgatttcg gcttgcacct gctcgcatca tcaggcgaaa
      121 caggaaaccg tatcggcttc ctgtcacgaa cattcggcga aaacagtcca aagtcgcgac
      181 gacgatttgc cggaaacaat cgaaacgtca atttccgaag ccggatgcgt ttgttttcag
      241 agcgcgcgga aggcttttgc caaatcggaa aacgttaaat ttgaaaaaca cgcggcggca
      301 atttcgcctt taacgccgcc cgcgattgtt tctatcccgc aaaacgctcg ggtaaagtta
      361 gatttcacaa aaccgtttta tctttccgat tctttctata atttctcgca cgggcgcgcg
      421 ccgccggttt tataaagcag aaaataccac acaaaaaagt tgggcaaatt gttttcgagc
      481 aatttgctac ggattgccgt ctgcaaaacg gcgatttccc gaaagactgt tttataaatc
      541 aggagatttt aaaatgaaaa cattattttt atcagcgatt ttcgcgcttt tactgggcgt
      601 gaacgccttc gcgcacgagc ccttcaagct cgtcagcagc gaaagaaaaa ccgtcagaga
      661 agccgatttg gcggctttgg aaaaaaatca gaccgtcggc gcaatagaca aaaccaatct
      721 gacttttact gcgaaagaga ttcgtctcgt cgccgtcacc ggtcccgaag acgatatgct
      781 ttcctaccgg atacaaggcg tccgaaatcc gaatttgatt gttccttcgg gcgcgctgct
      841 caggatttta tttgttaacg tcgatgtcga tatgcgccac gatattcgct tcggacacgt
      901 cgcgggcgat tttccgattg cgcccgaaat tgccggcacg gcaggcagtc aaaagctcga
      961 aaaaagagcc ggagacgacg ctttgcaggc ggaagaaatc gtcgtgcagg cgaaagaaga
     1021 cggcgctttt aaatatttct gctcaattcg cggacacgcc aaaggcggaa tgtggagcaa
     1081 tattttagtc gacgtcaaac cgggcggcaa tctgaaaacc gcgacgaaaa ccgaacacgt
     1141 tcacaccgaa gacgaagaca gggacacgga cgaaaaaaag acgaacgata aaaaaccgac
     1201 cgagcataat cacgatatga aaacggacga aatgaaaacg gacgacaaaa aatctgaaaa
     1261 tatgccggag atgaaaacgg acgataaaaa aatgtccgga atgccgcacg acgaaaagaa
     1321 aatgtccgcc attaccgaca tcaacgcgcc gatgtcgcgc gagagctccg gcacagcctg
     1381 gctgccggat tcatctccgc tgtacgggac gatgaaaatg tttaaagacg gaagtatgct
     1441 gatgtttcac ggcaacttat ttctgcgata cacgcgcgcc ggctcgcggc gcgatttttc
     1501 agccggcggc aaaggcgata atgcgcgttt cgatgcgccc tcgatgttta tgctgatgta
     1561 ttccaagccg attgaccgga aatcgcaaat cggcttgcgg gcgatggtca gcctcgaccc
     1621 ggttatcgag cgcggttacg gttatccgct gctttatcaa tcgggcgaaa cttatcgcgg
     1681 gcatccgctg cacgaccgcc agcacccgca cgattttttc tcggagcttt ccgcttcgta
     1741 ttcataccgg attaacgaga aacaatcgtt ttttttctac gccggttatc cgggcgagcc
     1801 cgctctgggt ccgccgactt ttatgcaccg cgtttcggcg atgaacaacg cggacgcgcc
     1861 tctttcgcat cactggcagg attcgacgca tattacctgg ggcgttttga cggcgggcta
     1921 caatttcgga aaatttaaat ttgaggcgag cgcgtttaaa ggacaggaac cggatgaaaa
     1981 ccgttggaat ctcgacgcgc cgaagctcga ttcgttcagc ggcagatttt cttttaaccc
     2041 gaataaggaa tgggcgtttc agatttcgta cggttatctg aaaaatcccg agccgtcgga
     2101 accgaatctg aagattttgc gtcgcacgac cgcttcggcg atttataata aaaagttcgg
     2161 cgacgataaa aactgggcga actcgttcgt ctggggacaa aatcacggca acggcgagcg
     2221 caccgacgct tttttgttcg agacggatta tcaattccgg aaaaattcaa ttttcgggcg
     2281 ggtcgaaaga gtcgaaaaat ccggtcacga actggctttg gacgaaatcg accacgataa
     2341 aatcttttgg gtcggcgcct attcgctcgg ctacgtgcgc gagctatacc ggaataaaaa
     2401 tctgaatgtc gggctcggcg cgcaggctgg tttttatcaa aacccgaacg gattaaacgc
     2461 ttattacggc ggcacgaaac atcaaagctg gcaaatcttt ttgcgccttc gcccgtcggc
     2521 gatgaagcac tgaggagaca aaatgcacga gaaacatttg cgcgaagaaa tgcgcgagga
     2581 taaatccgga acgcacatcg acccgatttg cggaatgacg gtcaacccgg cgaccgccgc
     2641 cggaagcttt aaacacgaag gcacgactta ttatttctgc tcgaaaggct gcctgcaaaa
     2701 atttatcaat cagacgagcg gcgcgccgcc gagcggtctg gttcagttag gcagaagaaa
     2761 agagattgtc cggcacggcg aaatgactgc cgcgccgcag ggcgaattta tcgacccggt
     2821 ttgcggaatg tcggtcgcgc cggaaaccgc ggcgggaaaa tacgattttc aaggacggag
     2881 tttttatttt tgctcgacgg gctgcctgca taaattcaag caaaatccgt cgaattttct
     2941 cgaaaagaaa aaagaggaaa cgaaagcggc ggcaagcgag ggcgtcgaat atacctgccc
     3001 gatgcacccg gaaatcgtac aaatcggcgc gggaacctgc ccgaaatgcg ggatggctct
     3061 ggaaccgaaa accgtctcgc tcgacgatgc gcccgacccg gaatatctgg atatgaaacg
     3121 gcgattctgg atttccgccg tgctgactct gccggttttt ctgctggcga tggcggaaat
     3181 gctgccgggt tttcattcgt ttatcgcgcc ggagcgcgcg ctctggattc aattcattct
     3241 ggcgacgccc gttgttttat attgcggatt tccctttttc gtgcgcggcg cgcaatcggt
     3301 taaaaactta agcccgaata tgtttacgct gattgccgtc ggcacgggcg cggcttatct
     3361 ttacagcctc gcggcggttt tctttccggg cgtttttccg gcttcgctgc acaatccgca
     3421 cacgcattta atcagcgtgt attttgaagc ggcggcggtg attacgactt tggttttgct
     3481 cggtcaggtt ttggagctgc gcgcccgcag ccagacttcg agcgctttaa aagagctgct
     3541 cggactcgcg ccgaaaacgg cgacagctct tttcgacgac ggaagcgaag cggagatttc
     3601 tctggcggac gttcagaaaa acgcgcgcct tcgcgtcagg gcaaatgaaa aaatcccgac
     3661 cgacggcgtg attttagaag gcgattcgac gattgacgaa tcgatggtga cgggcgagcc
     3721 gattccaatc gaaaaaacaa aaggcgaccg cgtcatcggc ggcacaatca acggcaatcg
     3781 cgctttcgtg atgcgggtcg aaaaagtcgg cagcgaaacg ctgctcgctc aaatcgtgca
     3841 gatggtcggc gaggcgcagc gctcgcgcgc gccgattcag agattagccg acatcgtttc
     3901 cgcttatttc gtcccggcgg taattgtcgt cgccatcgtt acatttctgg tctgggcttt
     3961 gctcggaagc tttacgagcg gctttgttta tgcggtcgcc gtgttgatta tcgcctgccc
     4021 gtgcgcttta ggattggcga cgccgatgtc gattatggtc ggaaccgggc gcggagcaaa
     4081 agcgggcgtg ctggtcaaaa aagcggaagc gctggagatt ctggagaaaa taaacacgat
     4141 tgtggtcgat aaaaccggca cgctgaccga aggcaaaccg cgcttgcagg cggtagtttc
     4201 gctgtcgggt tcggatgaaa acgaaatttt gagattcgcc gccagtctgg aaaaatcgag
     4261 cgagcatcct ttggcggagg cgattgtcaa aggcgctctg gaaagaaatg tcggtttcgc
     4321 caaagtctcg gattttcaat cgatcaccgg aaaaggttta attggaaaaa tcgacgggaa
     4381 agaaatcaaa ataggaaacg agaaatttct tgttcaggaa aatttcaaac aggatgaaca
     4441 ggataaaaat ccggcaaatc ccgtccgtcc tgtaaattcc gtttccatac gcgacaaagc
     4501 aaacgagctt cgcgcccaag gtcaaaccgt aatgtttgtc tcagttaacg gaaatctcgc
     4561 cgggcttgtc ggcgttgccg acacgataaa ggaatcggcg aaagaagcga ttgccggact
     4621 gcgccgtcag aaaatcgaaa tcgtgatgat gaccggcgat aaccaggcga cggcggaagt
     4681 tgtcgcccgc cagctcggca tcgacaaagt tttcggcgac gttctgccgg acgaaaaagc
     4741 aagcaaggtg aaagaactgc agg
//
LOCUS       DASWNE010000757         3224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_446842, whole genome shotgun sequence.
ACCESSION   DASWNE010000757 DASWNE010000000
VERSION     DASWNE010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446842"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="VGB68_14750"
     CDS             complement(<1..834)
                     /locus_tag="VGB68_14750"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY0050548.1"
                     /translation="MKVVLYTSFALVAFAFNSILCRLALGAEAIDAPSFTTIRLFSGA
                     MVLVLIKSFFGKKESEAKSGNWISAFFLFAYAICFSLAYINLTTATGALILFGSVQLT
                     MLGAALVKGERPQIPEWLGLFSAFAGLVYLVFPGLSAPPLLSCASMASAGAAWGFYTL
                     RGRASRNPLADTTGNFLRSVPMIILVALPFVYRIHLSPKGIIFAVLSGAIASGIGYSL
                     WYAALKYHAATRAAILQLCVPALAAIGGVIFLAEIISMRLWLASALILGGIALAILGK
                     RK"
     gene            complement(963..2528)
                     /locus_tag="VGB68_14755"
     CDS             complement(963..2528)
                     /locus_tag="VGB68_14755"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY0050549.1"
                     /translation="MKNPDAQNEPAGNHESVKTLRLWQMIYLGKGSFARQSLHAFISI
                     ALRLFFRRIEASNVEKVPKQGAVIFVLNHPNGAVDPALVFVSLPRRVSFLAKSTLFSI
                     PVGGFLLRTLEALPVYRRIDAAGDMSKNVRTFEVCRELLSRGRCIAIFPEGISHDETK
                     LQPMKTGAARIALGALSLDSTSSNEAEARASVKARNVAEAENHPQIDAASHNLKSDVP
                     GAVTENRFNLKIMAVGLYYTSKTAFRSEALIRYGEIFDVEPIEPDENGEPPRPAVQDL
                     TDKIEMALRNVTLNLESEQELDTVLKAEALFSSVYQNLIFKQTLTQSFQHWQNLAEKY
                     KLLGENEPEKMRRLNAKIARYENDLKTSGVTAESLSVLQHPTFYVLRYLVLRLLLLIM
                     LSPPAIVGAIIHSPAYVFSNFIGLMFRTHGADAAGSTYKILAACLFMPLTWLITAGVI
                     FWFFGWQFALASIPLTILCGYVALRSSETLIDMTVWLKSAWLLFRQKGLFLRLLLKRE
                     VLRQEIGELIEKD"
     gene            complement(2623..3027)
                     /locus_tag="VGB68_14760"
     CDS             complement(2623..3027)
                     /locus_tag="VGB68_14760"
                     /inference="COORDINATES: protein motif:HMM:NF018574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HEY0050550.1"
                     /translation="MFKDTESQLETIKEIFAQAKQEDNWNLDEPMLYSFYFVDESVDK
                     LEKLGLKLEADGYDFIDIFELGDEETNVSTGEYLLHIDKVEIHTPESLAERNVAFQKL
                     ADEYEIETYDGWEFGEVGDEDDEDNEEAGVEA"
BASE COUNT          856 a          840 c          772 g          756 t
ORIGIN      
        1 tttccttttt cctaaaatcg ccagagcaat tccgccgaga atcagcgcgc tcgccagcca
       61 taaacgcatt gaaataattt ccgccagaaa aatcacgccg ccgattgccg ccagcgccgg
      121 aacgcaaagc tgcaaaatcg ccgcgcgcgt cgccgcgtga tatttcagcg cggcatacca
      181 gagcgaatag ccgatgccgg acgcaatcgc gcccgataaa acggcaaaga taattccctt
      241 cggcgataaa tgaatccggt aaacgaaagg cagcgcgacc agaattatca tcggcaccga
      301 gcgcagaaaa ttccccgtcg tatcggctaa aggattccgg ctggcgcgcc cgcgcaaggt
      361 gtaaaagccc cacgcggctc ccgccgaagc catcgacgcg cagcttaaaa gcggcggcgc
      421 ggacaaaccc ggaaaaacca gatagaccag accggcaaac gctgaaaaca atccgagcca
      481 ttccggaatt tgcggtcttt cgcctttcac gagcgccgcg ccgagcattg ttaactgcac
      541 cgaaccgaac aaaattaaag cgcccgtcgc ggtcgtcaga tttatgtagg cgagcgaaaa
      601 acaaatcgcg taggcgaaaa gaaaaaaagc cgaaatccag tttccgcttt ttgcttccga
      661 ttccttttta ccgaaaaacg atttgattaa aaccagaacc atcgcgccgg aaaacagtcg
      721 aatcgtcgta aagctcggcg cgtcaatcgc ttccgcgccg agcgccagac gacacaaaat
      781 cgaattgaaa gcgaaagcga cgagcgcgaa agatgtatac aaaacgactt tcatcggata
      841 aaaagaagat tttaacagga tggacaggat aagcaggata aaaaatcaaa aatcaatgtt
      901 ttaaaatttt ccctatcctg tatatcctgt ccatcctgtt aaaatttcca cattaatcat
      961 tttcaatcct tctcaatcag ctcgccgatt tcctgccgca aaacttcgcg ttttaaaagc
     1021 aggcgcagga ataagccttt ttgtctgaac aaaagccacg ccgatttgag ccagacggtc
     1081 atatcaatca gcgtctcgga cgagcgcaga gcgacgtagc cgcacaaaat cgtcagcggg
     1141 atggaagcca gcgcaaactg ccagccgaaa aaccagaaaa tgacgcccgc cgtaatcagc
     1201 cacgtcagcg gcataaacaa acacgcggcg agaattttat aagtcgagcc ggcggcgtcc
     1261 gcgccgtgcg ttctgaacat caagccgata aaattcgaga aaacatacgc gggcgaatga
     1321 ataatcgcgc cgacgattgc cggcggcgaa agcataatta aaagcagcag gcgcaaaacc
     1381 agataacgca agacataaaa ggtcgggtgc tgcagcacgg aaaggctttc cgccgttacg
     1441 ccgctcgttt tcaaatcgtt ttcataacgg gcgattttag cgttcagccg acgcattttt
     1501 tccggctcat tttcgcccag gagcttgtat ttttccgcca gattctgcca atgctgaaag
     1561 ctttgcgtca gcgtttgctt gaaaatcagg ttttgataaa ccgacgaaaa caaggcttcg
     1621 gctttgagaa ccgtatcgag ttcctgttcg gattcgagat tcagagtgac gtttcttaaa
     1681 gccatctcga ttttgtcggt taaatcctgt acagccgggc gcggcggctc gccgttttcg
     1741 tccggttcga ttggttcgac atcgaaaatt tcgccgtaac gaatcagcgc ttcgcttcga
     1801 aaagcggttt tggaggtgta ataaagcccg accgccatta tttttaagtt aaaacggttt
     1861 tcggtcactg cgcctggcac gtcggatttc agattgtgtg aagctgcgtc tatctgcgga
     1921 tggttttccg cttccgcgac atttcgcgcc tttacggaag cccgcgcttc tgcctcgttt
     1981 gacgaggttg agtcgaggct gagcgcgccg agggcgattc gcgccgcgcc ggttttcatc
     2041 ggttggagct ttgtctcgtc gtgcgaaatg ccttcgggaa aaattgcgat gcagcgtccg
     2101 cgacttaaaa gctcgcggca aacttcaaaa gtccgcacat ttttcgacat atcgcccgcc
     2161 gcgtcaatcc gccggtaaac cggaagcgcc tccagcgtcc gcagcagaaa tccgccgacg
     2221 ggaatggaaa aaagcgtgga tttggcgaga aacgaaacgc ggcgcggcag actgacaaac
     2281 accagcgccg ggtcgaccgc gccgttcgga tgattgagaa caaagattac cgcgccttgt
     2341 ttcgggactt tttcgacatt gctcgcttcg atgcgccgaa aaaaaagtcg cagcgcgatg
     2401 ctgatgaaag cgtgaagcga ttggcgggca aacgagcctt taccgagata aatcatctgc
     2461 cacaaacgca aggtctttac gctctcgtga tttccggcag gctcattttg tgcgtctgga
     2521 tttttcaaat tttatccggg aaatttaccg aagacataag gagccgagga taaaagaaaa
     2581 taactaaatt tcctttatct tctttatccc ggtcaattat tcttatgctt cgacgcccgc
     2641 ttcctcgttg tcctcgtcgt cttcgtcgcc gacttcgccg aattcccagc cgtcgtaggt
     2701 ttcgatttcg tattcgtcgg cgagcttttg aaacgcgacg tttctttcgg ctaacgattc
     2761 gggcgtgtga atctcgacct tgtcgatatg aagcagatat tcgccggtcg atacgttggt
     2821 ctcctcgtcg ccgagctcaa aaatgtctat gaaatcatag ccgtcggctt cgagcttcag
     2881 accgagcttt tccagcttat cgacgctttc gtcgacgaaa taaaagctgt agagcatcgg
     2941 ctcgtccaga ttccaattgt cttcctgttt tgcctgcgca aaaatttcct tgattgtttc
     3001 cagttgcgat tcggtgtctt taaacatatt tcttcccttt gtcgatagat ttgttaataa
     3061 acaataagtc taacaaagca cgcgaagatt ttcacaacac gctccgatta ataatcacga
     3121 ccggagccgc aaaagttgtt tgcgacttcg tttataaact gatttttcag aaataacttc
     3181 aaacggtttc gaattttgaa aaggctttca ataaaaaaag gcga
//
LOCUS       DASWNE010000758         8889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_590459, whole genome shotgun sequence.
ACCESSION   DASWNE010000758 DASWNE010000000
VERSION     DASWNE010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8889
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590459"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VGB68_14765"
     CDS             complement(<1..319)
                     /locus_tag="VGB68_14765"
                     /inference="COORDINATES: protein motif:HMM:NF019606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEY0050551.1"
                     /translation="MSNRKLGIAIVGLGGAVGTTMVAGIELLKKGLIGTEGLPLAELD
                     ENLTGDLAAYDSLVFGGWDLFGEHLAKAAEEHDVLTHKQFVAVENELREIKPWRAVGN
                     REFL"
     gene            457..1023
                     /locus_tag="VGB68_14770"
     CDS             457..1023
                     /locus_tag="VGB68_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050552.1"
                     /translation="MKNFISVISAIALLAVLGAAISINETNLSVEREITASNESAPRE
                     CLKIFEEFFQYVYKGKSDIVKDMKAQDKWLSKNLKNAFTKHVEASGKPEENPDYPSNA
                     GFVGAWEYPAGYSVVGSRKYGNRAIIDVNYKWGKGQNYEGDERQMSFIFVFEEKSWKL
                     DDVYTFKSEYSSEQSLAYYFKNSRTLKN"
     gene            complement(1027..2085)
                     /locus_tag="VGB68_14775"
     CDS             complement(1027..2085)
                     /locus_tag="VGB68_14775"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEY0050553.1"
                     /translation="MSHDTKPIRAGEELNEAGLLAFLRENLGARDRQIEISQFPAGSS
                     NLTYCIKIGADEYVLRRPPFGNQVKSAHDMSREFNVLSKLSKVYAAAPEPLIYCADEA
                     VLGSEFYLMQRRNGLIIRGKSPEILESSPELQKKVCESFVENLANLHALDYRKIGLGD
                     LGKPEGYARRQVEGWTRRYFNAKTDENIELEKAIRWLNANIPASSSAALVHNDYKFDN
                     VMLNPENLTEITAVLDWEMTTVGEPLMDLGTTLGYWMSKEAGIEMLSMPFNPRVLMEN
                     ISRRELIEIYAEKSGRDLSGMLFYYAFGTFKIAAIAQQIYFRFARGFTRDRRFAAFNH
                     FVRALGKIALHAIETEKI"
     gene            complement(2082..2471)
                     /locus_tag="VGB68_14780"
     CDS             complement(2082..2471)
                     /locus_tag="VGB68_14780"
                     /inference="COORDINATES: protein motif:HMM:NF022443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2809 domain-containing protein"
                     /protein_id="HEY0050554.1"
                     /translation="MKFAFSLKYLLLAAVLFAVEVCIALFVKDAIVRPFVGDALVVIL
                     IYCFLRIFSDVANWKLALGVLVFACLVEILQHFDYVARLGLENNRVLSVMMGRTFEWM
                     DFVAYLAGFLFIILIDFFYRRKIRMDL"
     gene            2557..3747
                     /locus_tag="VGB68_14785"
     CDS             2557..3747
                     /locus_tag="VGB68_14785"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0050555.1"
                     /translation="MNFDYSTKTRELQKRLQDFMDKNIYPNESAYTREINSGDRWQPL
                     RLIEDLKEKARADGLWNLFLPEVSGLTNLEYAPLAEQMGRVYWSAEVFNCSAPDTGNM
                     EVLERYGSAEQKDAWLKPLLNGEIRSCFAMTEPDVASSDATNICSSIKKDGDRYVLNG
                     RKWWTTGAGDARCKLAIFMGKTDEKAPKHLQQSMILVPLDAGGVRVERILNVFGYDDA
                     PNGHAEISFENVRVPAANLILGEGRGFEIAQGRLGPGRVHHCMRLIGVAERALELMCE
                     RAKARTAFGKTIAEQGVVRNWISEARLEIEQARLLVLKTAWMMDEVGNKAARREIAMI
                     KAVVPRAALKIIDRAIQAHGGGGVSQDFPLANFWIIARTLRLADGPDEVHLESIAKME
                     LKKY"
     gene            3748..4239
                     /locus_tag="VGB68_14790"
     CDS             3748..4239
                     /locus_tag="VGB68_14790"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0050556.1"
                     /translation="MNVRCVTPGDAAQIAGIYNYYIETSHATFELDAIDSAEMENRMR
                     ESLARDYPFFVCEANGEILGYAYGRQYRPRRAYRHSIEVSVYIKNGAQGNGIGTRLYE
                     RLFPEIRQRDFHAIIAGISLPNEASIRLHEKFGFEKVAHFREVGFKFGGWIDVGYWEL
                     IKR"
     gene            4610..5491
                     /locus_tag="VGB68_14795"
     CDS             4610..5491
                     /locus_tag="VGB68_14795"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0050557.1"
                     /translation="MKTRNLQVINEETAADSASLETKKEPASSPSYVDLNNSGVKKAL
                     AEGDYKQAITLFLRAVELKPDCFTCRLNLGRSFLKLEKYDDAVGIFNQLTKIAPDNAN
                     AQASLGEALNAKGFYKESLPVFREALKLNSKDAITLCNYADALNLTGSYEEALQRLDE
                     SIRLQPDFAEAYGSRGYTLYALGRYKEALAALKKAEALNPKLPQVHNNLGVVHDRLGK
                     KKEALKHYLEAVRLQPDFAAAICNLAASHLANGNREAAYEQLKVLEKIDSRLAEQLRG
                     AMWGKYVINASETKKDN"
     gene            6238..7293
                     /gene="pilM"
                     /locus_tag="VGB68_14800"
     CDS             6238..7293
                     /gene="pilM"
                     /locus_tag="VGB68_14800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HEY0050558.1"
                     /translation="MLFGKKKSVAGLDVGSSAVKVVELEGKMNSLNLVSLGFENLPDN
                     TIVDGQIMELNTVSDVIQNVCNDHQIKAPQVVTGVSGHSVIVKNIVLPPMSRDELEES
                     IDWHAEEHIPYDLADVSLDYHITAETSEATTVLIAACKRERIDNLKQVIQFAGKQAVV
                     IDVDTFALQNCYEVNYQPSDTEVVTLLNIGASTMNVNIVQGNRSLFTRDITVGGSQFT
                     DVLQRSLGLSYQQAEAVKRGVVDAADGVEEKSIEPLMDNVMEMVAMEIQKTFDFYRAT
                     SEDGGVPVQKILISGGGSKLAGLPEELSTRLELPVEVLDPFRQIKVDARKFDPDYLSE
                     IMPEMAVAVGLALRGVE"
     gene            7298..8074
                     /locus_tag="VGB68_14805"
     CDS             7298..8074
                     /locus_tag="VGB68_14805"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEY0050559.1"
                     /translation="MIKINLLESVTDKQASAVVAVEKKVGSPTSRLLLMALVVTVLTI
                     TVIGWDIVSSQMAKAEAERQLEEQKKIALELDAVLKEQKELEAKIASIDSRINAIKTL
                     RASQSGPSSVLEAVRERIGMVAGLYLESIEQKGDQLTIKGNSPDEAAVTQFGRSLEFS
                     SGLFSNLNIETQRKEVQSNQVAAPTGMASGAAEVPKLETVNFTIRTSYTPGGKTNPDQ
                     QPTQANVTPGQTPNAPNAQTPNAQTPPNAANPQVARNQDK"
     gene            8132..8803
                     /gene="pilO"
                     /locus_tag="VGB68_14810"
     CDS             8132..8803
                     /gene="pilO"
                     /locus_tag="VGB68_14810"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEY0050560.1"
                     /translation="MEKFKSLAWHWQLLILVGVGSIIYLGVWYFMTSVTRAETQTIND
                     EVSQLQAKNQAAQIATQRINEFRSLYASKSAEYDELKVLLPEVREITNVLQGLQDTAR
                     DSRLIVMRFSPRDDTQQDGFMAKPVEVEVDSNFNNLRAFFEKMAKLQRIVSVTDFNLK
                     QLEKQSPDKTLHAQFLLTAYYASPENLTATNNPQQPGANPTAPAPNGQPVAPNAQTPP
                     APVAK"
BASE COUNT         2403 a         2194 c         2244 g         2048 t
ORIGIN      
        1 ccagaaactc gcggttcccg accgcgcgcc agggcttgat ttcccgcagt tcgttttcga
       61 ccgcaacgaa ttgtttgtgc gtcaaaacgt cgtgttcttc ggcggctttc gccagatgct
      121 cgccgaacaa atcccagccg ccgaaaacca aactgtcgta agccgccaaa tctccggtca
      181 aattttcgtc cagctcggcg agcggcaagc cttccgtgcc gatcaagcct ttttttaata
      241 attcgattcc ggcgaccatt gtcgtgccga ccgcgccgcc taatccgaca atggctatgc
      301 cgagttttct attgctcata aaaaccttaa ttctacaaca aaatcaaatc agccacgaat
      361 tagcaggaat cgtcacgaat aatttttcga agtttaccct ggagtttgag aaaaaatcgt
      421 tgactttcgt gaccttcagg atttacattg cgctttatga aaaactttat ttcagtaatt
      481 tccgcaatcg cgctgctggc ggttttgggc gcagcgattt ctattaatga aacgaatctt
      541 tctgtcgagc gagaaatcac ggcttcaaac gaaagcgctc cgcgcgagtg tttgaaaata
      601 tttgaagagt tttttcaata cgtttacaaa ggtaaatccg atattgtgaa agatatgaaa
      661 gcgcaggata aatggctgtc gaagaatttg aaaaacgcgt ttacaaagca cgtagaggct
      721 tccggcaaac cggaagaaaa tcctgattat ccttcaaatg ccggttttgt cggggcttgg
      781 gagtatcccg ccggttattc cgtggtcggc tcgagaaaat acggaaaccg agccattatc
      841 gatgttaatt ataaatgggg aaagggacaa aattatgaag gcgatgaaag gcagatgtcc
      901 tttattttcg tttttgaaga gaaaagctgg aaactcgacg acgtttatac ttttaaaagc
      961 gaatattcat ccgaacaaag tctggcgtat tatttcaaaa actccaggac tctcaaaaat
     1021 taattactaa atcttttccg tttcaatcgc gtgcagggcg attttgccga gcgcgcggac
     1081 gaaatgatta aacgcggcaa agcgcctgtc gcgcgtaaag cctctggcga agcgaaagta
     1141 aatctgctgg gcgatggcgg cgattttgaa tgtgccgaaa gcgtaataaa aaagcattcc
     1201 ggagaggtcg cgaccggatt tttcggcgta gatttcaatt agttcgcggc gggaaatgtt
     1261 ttccatcaaa acgcgcggat tgaaaggcat cgaaagcatc tcgatgcccg cttctttcga
     1321 catccagtaa cccaaagtcg ttcccaaatc catcagaggc tcgccgacgg tcgtcatttc
     1381 ccaatccagc acggctgtga tttccgtcag attttcggga ttcagcatca cgttgtcgaa
     1441 tttgtaatcg ttgtggacga gcgcggcgct gctcgacgcg ggaatattcg cgttcagcca
     1501 tcggatggct ttttccagct cgatattttc gtcggttttg gcgttaaaat aacgccgcgt
     1561 ccagccttcg acctgacggc gcgcgtaacc ttcgggtttt cccaaatcgc cgagaccgat
     1621 tttccggtaa tcgagcgcgt gcagattcgc cagattttcg acgaagcttt cgcaaacttt
     1681 tttttgcagt tccggcgagc tctccagaat ctcgggcgat ttcccgcgaa taatcaaacc
     1741 gttccggcgc tgcatcaaat aaaactccga gccgagcacc gcttcatcgg cgcagtaaat
     1801 caaaggctcg ggcgctgccg cgtaaacttt cgaaagcttt gataaaacat taaactcgcg
     1861 cgacatatcg tgcgccgatt tgacctgatt gccgaacggc gggcggcgca aaacgtattc
     1921 gtccgcgccg atttttatgc agtaagttaa attcgagctg cccgccggaa actgcgagat
     1981 ttcgatttgc cgatcgcgcg cgccgagatt ttcccgcaaa aacgccagca gtccggcttc
     2041 gttcagttct tcgcccgcgc gaatcggttt tgtgtcgtga ctcataaatc cattcgaatt
     2101 ttccgcctgt aaaaaaaatc aatcagtata ataaataaaa atcccgccag ataggcgacg
     2161 aaatccatcc attcgaaagt tctgcccatc attacggaaa gcacgcggtt attttccagc
     2221 ccgagccgcg cgacgtaatc gaaatgctgc aaaatttcga cgagacaggc gaaaacgaga
     2281 actccgagcg ccaatttcca attggcgacg tccgaaaata ttctcaaaaa acaatagatt
     2341 aaaatgacga ccagcgcgtc gccgacaaac gggcgaacaa ttgcgtcttt cacaaacagc
     2401 gcgatgcaga cttcgacggc aaacagcacg gcggcgagca aaagatactt cagactaaaa
     2461 gcaaacttca tttttcttcc gccgcatccg ccgaatattt tcccgccggc gcgattttca
     2521 tacaaaattt tccgtgttaa tctgtggctg aattttatga atttcgatta ttcaacgaaa
     2581 acgcgcgaac tgcaaaaacg tcttcaggat tttatggata aaaacattta tccgaacgaa
     2641 agcgcttata cgcgggaaat aaattcgggc gaccgctggc agccgctgcg attgattgag
     2701 gatttaaagg aaaaagcgcg ggctgacggt ttgtggaatt tgtttctgcc ggaagtttcc
     2761 ggtttgacga atctggaata cgcgccgctc gccgaacaga tgggcagagt ttactggtcg
     2821 gcggaagttt tcaactgctc cgcgcccgac acgggcaata tggaggtttt ggaaagatac
     2881 ggctcggcgg agcagaaaga cgcgtggctc aagcctttgt taaacggcga aatccgctcg
     2941 tgtttcgcga tgaccgagcc tgatgtcgct tcgtccgacg cgacaaatat ttgttcgtct
     3001 attaaaaaag acggcgaccg gtatgtttta aacggcagga aatggtggac gaccggcgcg
     3061 ggcgacgctc gctgcaagct ggcgattttt atgggcaaaa cggacgaaaa agcgccgaag
     3121 catcttcagc agtcgatgat tctggttccg ctcgacgccg gcggcgtcag ggtcgaaaga
     3181 attttgaatg ttttcggcta cgacgacgcg ccgaacgggc acgccgaaat ttcgtttgaa
     3241 aacgtgcgcg ttccggcggc aaacttgatt ctcggcgaag gtcgcggctt tgaaatcgcg
     3301 cagggacggc tcggtcccgg acgcgttcat cattgtatgc ggctgatcgg cgtggcggag
     3361 cgcgctctgg aattgatgtg cgaaagagcg aaagcgcgca cggctttcgg caagacgatt
     3421 gccgagcagg gcgtcgtcag aaactggatt agcgaggcgc gtctggaaat cgagcaggcg
     3481 cggctgctgg tcttgaaaac cgcgtggatg atggacgaag tcggaaacaa agcggcgcgc
     3541 agggaaattg cgatgataaa agccgtcgtc ccgcgcgcgg cgctgaaaat catcgaccgc
     3601 gcgattcagg cgcacggcgg cggcggcgtc tcgcaggatt ttccgctggc gaatttctgg
     3661 attatcgcca gaacgttaag gctcgccgac ggacccgacg aagttcacct ggaatcgatt
     3721 gcgaaaatgg aattgaaaaa atattaaatg aacgttcgct gtgtcacgcc cggcgatgcc
     3781 gcgcaaatcg ccggaattta taattattac atcgaaactt cgcacgcgac ttttgagctg
     3841 gacgcgatag attcggctga aatggaaaat cggatgcggg aatctctcgc gcgcgattac
     3901 ccgtttttcg tctgcgaagc gaacggcgaa atcctcggtt acgcttacgg gcggcaatac
     3961 agaccgcgcc gcgcttaccg tcattcaatc gaagtctcgg tttatataaa aaacggcgcg
     4021 cagggcaacg gaatcggcac gcggctctac gaaagattat ttcccgaaat tcggcaaagg
     4081 gattttcacg caatcatcgc cggaatctct ttgccgaacg aggcgagcat caggctccac
     4141 gaaaaattcg gctttgaaaa agtcgcgcat tttcgcgaag tcggttttaa attcggcggc
     4201 tggattgatg tcggatactg ggaactgata aaaagataaa accgcaaccc tgcattgttt
     4261 ctgcattctt tcctgaaact aattacatat taaaccgtag tttattggca gcagctttca
     4321 agacagatgc tccgaccgga tttgtggaaa gccgaatcat accttttttt gtcataaaac
     4381 cggattcggc ggtgtttcaa ctgtagaaag ccatttctta aacgctaaaa atccattttc
     4441 gggaaaagcc ggttgcggct taaaaagagc tttccttttt tgctcaaaac cgttctttga
     4501 attaatacgg acaaacgatt attcgtttgc tttaaggaga ataatcatca tgcagaaaat
     4561 atatatactt ctggcagtca taacgttgtc attgtcaata aacgcgcaga tgaaaacccg
     4621 caatttgcag gtcatcaacg aggaaacggc agccgattcc gcttcgctcg aaacaaagaa
     4681 ggaaccggca agctcgccga gctatgtcga tttgaataat tcgggcgtta aaaaggcgct
     4741 cgccgaaggc gattataaac aggcgattac attatttttg cgggcggtcg agcttaaacc
     4801 cgattgcttc acctgccggc ttaatctggg tcgctctttt ctgaaactgg aaaaatacga
     4861 cgacgcggtc gggattttca atcagctaac gaaaatcgct cccgataacg ccaacgctca
     4921 ggcgagcctg ggcgaagcgc tgaacgcgaa aggattttat aaagaaagcc tgcccgtttt
     4981 tcgcgaggct ttaaagctga attccaaaga cgcgataacg ctctgcaatt atgccgacgc
     5041 tttgaatttg accggctctt atgaagaggc tttgcagcgg ctcgacgaat caatcaggct
     5101 gcagcccgat tttgccgagg catacggcag ccgcggttat acgctttacg cgctcggtcg
     5161 ttataaggaa gcgctcgccg cgctgaaaaa agcggaagct ctcaatccca aactgccgca
     5221 ggttcacaac aatctcggag tcgtccacga ccggctcgga aaaaagaagg aagccctgaa
     5281 acattatctc gaagccgtcc gtctgcagcc ggattttgcc gcggcgatat gcaatctggc
     5341 ggcgagccat ctggcgaacg gcaatcgcga agcggcttac gagcagctga aagtcctcga
     5401 aaaaatcgat tcccggctag ccgaacaact gcgcggcgcg atgtggggca aatatgtgat
     5461 taacgcttcg gagacaaaaa aggataactg aatctgattt ctgaaaaata aatctttgcg
     5521 tcctttgcgt taaaatcttt ctttaaccgc aaagaacgca aagattttcg cggggcaacg
     5581 caaagagatt tttaatagtt tgtgcgaaaa aattttgccg gcaagattgt ttcggacaaa
     5641 tgaaaaaacc gaaaattcat tttgctcacg ccaacagttt tcccgcgccc gcgccggtgc
     5701 gaaaactgcg aaaactttgc ccggctcata cgcctagaga aacccgcgcc gttgcctgac
     5761 gcgcgcggcg tccgccttta ttgtttcaat actgctttta tacggactgg ctttcactat
     5821 ttaatttagc cgtatttatt caatctgacc gaatgctctc atcatcttgg aagaaaaact
     5881 ttcattttgc tgttaaacaa tgcaaatact tgttgacaat gtttgttaaa tcttgtagtt
     5941 taattttaac agcagcaaat actgcgctgt tcctatcgtc acctgaaagc acaaaaggta
     6001 tccaaatttc gcatacacaa gagtttatta aatgttgcgc tcatcaaaag atggaaaaca
     6061 aacgggtgtt ttggcttgag tcctacagtt tagcctgtcc gagagaagtc ctgtctagag
     6121 ttgctggttt cgccaaggtg acaaaatcca ttcaaaagaa aactaaaatg cccgtttaat
     6181 tttttaaatt tcaatcgcgc gccgcgataa aaaagctaaa agagtaagga gaagataatg
     6241 ctgttcggta aaaagaaaag tgtcgccggt ttagatgtcg gctccagtgc tgtcaaagtc
     6301 gtcgaactcg aaggcaaaat gaatagcctg aacctggtca gtctgggttt tgaaaacctg
     6361 cccgacaaca ccatcgtcga tggtcagata atggagctga acacggtttc agacgttatt
     6421 caaaacgttt gcaacgacca tcagataaaa gctccgcagg tcgtgacggg cgtcagcggt
     6481 cactcggtga ttgttaaaaa tatcgttctg ccgccgatga gccgcgacga gctggaagaa
     6541 tcgattgact ggcacgccga agagcatatt ccatacgatt tagcggacgt cagcctcgat
     6601 tatcacatca cggcggagac ctccgaagcc actacggttt taatcgccgc ctgcaagcgc
     6661 gagcgaatcg acaacctgaa acaggttatt caatttgccg gaaaacaggc ggtcgttatc
     6721 gacgtcgata cttttgcgct gcaaaactgc tacgaagtca actatcagcc gtcggatacg
     6781 gaagtcgtga ccttgcttaa tatcggcgct tcgacgatga acgttaacat cgtgcagggc
     6841 aatcgctcgc tgtttacgcg cgacatcacg gtcggcggca gccagtttac cgacgttttg
     6901 cagagaagtc tgggcttgag ctaccagcaa gccgaagcgg tcaagcgcgg cgtcgtggac
     6961 gcggcggacg gcgtcgaaga aaaatccatc gagcctttaa tggataacgt gatggaaatg
     7021 gtggcgatgg aaattcaaaa aactttcgat ttctatcgcg cgacctccga agacggcggc
     7081 gttcccgtcc agaaaatttt gatttcgggc ggcggctcaa agctcgccgg attgcccgaa
     7141 gagctttcga cgcgcctgga gctgccggtc gaagttctcg acccgtttcg tcaaatcaag
     7201 gtcgatgcca gaaagttcga ccccgattat ttaagtgaaa ttatgcctga gatggcggtc
     7261 gccgtcggtc tggcactaag aggagtggaa taagaagatg attaagataa accttttaga
     7321 atctgttacc gacaagcaag cctcagccgt cgtcgccgtc gaaaagaaag tcggcagccc
     7381 gacttcgcgc ctgcttctga tggcgctggt cgtaacggtt ttgacgatta ccgtcatcgg
     7441 ctgggacatc gtttcatcgc agatggcaaa agccgaagcc gagcgccagc tcgaagaaca
     7501 aaagaaaatc gctctggaac tggacgcggt attgaaagag cagaaagaac tggaggcaaa
     7561 aatcgccagc atcgactctc gcatcaacgc cattaaaacc ttgcgcgcct cgcagtccgg
     7621 tccgagctcg gtgctggaag ccgtgcgcga aagaatcggt atggtggctg gactttatct
     7681 ggaaagcatc gagcagaaag gcgaccagct gacgattaaa ggaaactcgc ccgacgaagc
     7741 cgccgtcacc caattcggac gcagcctcga attttcttcc ggtcttttct cgaacctgaa
     7801 catcgagacg cagcgaaaag aagttcaaag caatcaggtc gccgcgccga ccggtatggc
     7861 gagcggcgcc gccgaggttc cgaaactgga gacggtcaac tttacgattc gcacctctta
     7921 tacgccgggc ggaaaaacga atcccgacca gcagccgacg caggcaaatg tgacgccggg
     7981 acaaacgccc aatgcgccga atgcacaaac gccgaacgca caaaccccgc cgaacgcggc
     8041 aaatccgcag gtggcaagaa atcaggataa ataattcttt atctccagcc gattcgtttg
     8101 gcaggaaagt aacagaacga ggaaaactac gatggaaaaa tttaaatcac tcgcttggca
     8161 ttggcagctt ctgatattgg tcggcgtcgg gtcgataatt tatctgggcg tctggtattt
     8221 tatgaccagc gtgacgcgcg ccgaaactca aaccataaac gacgaagtat cgcagctgca
     8281 agccaaaaat caggcggcgc aaatcgccac acagcgaatc aacgaatttc gctcgttgta
     8341 tgccagcaaa tcggcggaat acgacgaatt gaaagttttg ctgccggaag tgcgcgaaat
     8401 caccaacgtt ttgcagggac ttcaggacac cgcccgcgac agccggctga tagtgatgcg
     8461 cttttcaccg cgcgacgaca cgcagcagga cggatttatg gcaaagccgg tcgaggtcga
     8521 agtcgacagt aatttcaata acctgcgcgc cttttttgaa aaaatggcga aactgcaaag
     8581 aatcgtctcg gtcaccgact tcaatctgaa acagctggaa aaacagtcgc ccgacaaaac
     8641 gctccacgcg cagtttttgc tgacggctta ttatgcttcg cccgaaaatt taacggcgac
     8701 taacaatccg caacagcccg gcgccaaccc gaccgcgccc gctccgaacg gtcagcccgt
     8761 cgcgcccaac gcgcagacgc cgcctgctcc cgttgccaaa taaatctaaa agctaaagag
     8821 ttttgttgaa gagagaaaaa gccatgatga aaaaatcgaa aatgttacgt ttcttaagtt
     8881 caagtgtcg
//
LOCUS       DASWNE010000759         4361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_236242, whole genome shotgun sequence.
ACCESSION   DASWNE010000759 DASWNE010000000
VERSION     DASWNE010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236242"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..735)
                     /gene="yihA"
                     /locus_tag="VGB68_14815"
     CDS             complement(154..735)
                     /gene="yihA"
                     /locus_tag="VGB68_14815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEY0050561.1"
                     /translation="MKILSSEFVKSSFASEHWTTDGRPEISFLGRSNVGKSSLINSLL
                     QRKGLARTSNTPGRTQSINFFLINERFYFVDLPGYGYAKVSKTMRADWGVMAEEYLSQ
                     RRELVLSVQLIDSRHSPTGLDRDLNEWLVFHQKNHIIVATKADKLSSNKLRKSLDEIQ
                     NVLPTSKIIPYSSETGKGRDAVWREIENALAHG"
     gene            813..1652
                     /locus_tag="VGB68_14820"
     CDS             813..1652
                     /locus_tag="VGB68_14820"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HEY0050562.1"
                     /translation="MSKSDSTLIAIGGGEFSEASDILEEFLGLLKNKSDARIVVMTIA
                     TNQPEDAARKYNSLFRARGINHVNTVDVDERDDAFKESALKKIRDADALFFTGGDQLN
                     VTSLLGGTPLHNVIYERYTEGFVIAGTSAGAMMMSNSMITEGRNDVAPRVSAIKIAPG
                     MDLISETIIDTHFSQRGRHGRLLTAIAHYPQDLGIGIDEKTAICVTGNEFKVLGNGVV
                     TVMDGNQMRHSNLAYRHDNEPLGLFGVCTHILPAGYKYDLKKREPISPALKNMTAPAA
                     NED"
     gene            1658..2575
                     /locus_tag="VGB68_14825"
     CDS             1658..2575
                     /locus_tag="VGB68_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050563.1"
                     /translation="MRLVMVNAPEGRGKDIAQIAFSADISKVSHRQVTSHHADGKTET
                     KDVVDVETSTPKAKRYIDGVLAADFYNPEDVTISVRQPRAIISKDDLRELTKPLVEPA
                     TDIFEELWQFNHITYGFVGRIFLAACLLAYGLIQQQTLLIIAGLLFLPLLPLLLAVGF
                     GSWTRNWKLSIQGVLAFLTATILLMAGGAAIAAVSDPPLKYSEFNSILVGFLISVAVG
                     IAAGLANIDDVGRREMIGLAATAQIAILPVWFGICLVFGFPATMSESEITNRALSFLI
                     NVPTIIVVSLAVYVLTGAASRFLARLKDR"
     gene            2582..3772
                     /locus_tag="VGB68_14830"
     CDS             2582..3772
                     /locus_tag="VGB68_14830"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0050564.1"
                     /translation="MLTLIENGEIYSPEPMGKGSILLVENQILKVGEINRRAVESLGV
                     EFEIIDARNCLISPGFIDPHLHLLGGSGEKGFSTQTPEISATEIIGAGITTVVGCLGV
                     DTTMKTMPGLLAKAKALKEEGLNAFIWTGGYNVPPTTISKSARDDIMFIEEVIGAGEI
                     AISDERSTEPDFHELAHLAHDAYVGGMLSKKAGVTHFHVGETGKRLHLLRDLIDKDDC
                     KPEWLYPTHITRSEELMLEAIALAARGCFVDIDTVEEDLEKWLKFYKDNGGDMKKLTV
                     SSDSSITSPATFFEQIRACVVGQKFTLEEVLPLVTANTAKALKLDAKGTIEIGKSADC
                     LVLEKDSFELREVIAGGRRLFKDGKIAFKEAFLKDSNRSINLTGEKKPKRAKASKAKG
                     TRQK"
     gene            complement(3822..4262)
                     /locus_tag="VGB68_14835"
     CDS             complement(3822..4262)
                     /locus_tag="VGB68_14835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050565.1"
                     /translation="MNKAESADLILKLYDLRREEKMREARSWITSFFPESAADIMQVM
                     VSAETSAYFRMVTSYWDMAASFVNHGAIDEEMFLESNGECVLIFSKVEPFLEEMRQIM
                     GSPNYLKNLETLIMKMPDAKQMLAARREMMKRWMQMRAETAKTA"
BASE COUNT         1087 a         1134 c         1085 g         1055 t
ORIGIN      
        1 tcaaaaccct gagttttact gagatgccta attattaccc agtttaactc aagaaatcaa
       61 ctaaaaaata gcgatttctt tattttaaaa tattaatccg tccaaagtta caaaaaattc
      121 atacgccgaa cccgattttc aattatttgc ggcttagccg tgcgccagcg cgttttctat
      181 ttcgcgccag accgcatctc tgcctttgcc cgtttccgac gaatacggaa tgattttgct
      241 ggtgggcaaa acattctgga tttcgtctaa actttttcgg agtttgttgg atgagagttt
      301 atcggctttg gttgcgacga ttatgtgatt tttttgatga aaaacgagcc attcgttcaa
      361 gtcgcggtcg agtccggtcg ggctgtggcg cgaatcgatt aattgaaccg acaaaaccag
      421 ctcgcgtctt tggcttaggt attcttccgc cattacgccc cagtcggcgc gcatcgtttt
      481 cgacactttc gcgtaaccgt agccgggcaa atcgacgaaa taaaatcttt cgttaatcag
      541 aaaaaaattg atgctctgcg ttctgcccgg cgtgttcgaa gttcgcgcca agcctttgcg
      601 ctgcaaaagc gaattgatga gcgaagattt gccgacgttc gagcgtccga gaaaggaaat
      661 ttccggtctg ccgtcggtcg tccaatgttc gctagcgaac gaacttttaa caaattcgga
      721 tgataaaatt ttcataacat ctttaattga acatcttaaa gatattaaaa tagaaacaca
      781 accaagttta cgaacaaggg gaaagaaaaa atatgagcaa atccgattcg acactgattg
      841 ccatcggcgg cggtgaattt tcggaagctt ctgatatttt ggaagaattt ttaggtcttt
      901 tgaaaaataa atccgacgct cgaatcgtcg tgatgaccat cgccaccaac cagcccgaag
      961 acgccgctcg aaaatacaat tctctgtttc gagcccgcgg aattaatcac gttaataccg
     1021 tcgacgttga cgaacgcgac gacgctttca aagaatccgc attaaaaaaa atccgcgacg
     1081 ccgatgccct tttttttacg ggcggcgacc agttaaacgt gaccagcctg ctcggcggca
     1141 cgccgcttca taatgtgatt tacgaaagat acacggaggg cttcgtcatc gccggaacga
     1201 gcgcgggcgc gatgatgatg tcgaactcga tgattaccga aggcagaaac gacgttgcgc
     1261 cgcgcgtcag cgccattaaa atcgcgcccg ggatggattt aatcagcgaa acgattatcg
     1321 acacgcattt ttcccagcgc gggcggcacg ggcgactgct gacggcgatt gcgcattacc
     1381 cgcaggattt gggaatcgga atcgacgaaa aaacggctat ctgcgtcacc ggcaacgagt
     1441 ttaaagttct cggcaacggc gtcgtcacgg taatggacgg caaccagatg cgccacagca
     1501 atctcgccta ccggcacgac aacgagccgc tcggactgtt cggcgtgtgc acgcatattc
     1561 tgcccgccgg ttataaatac gatttgaaaa aacgcgagcc gatcagcccg gcattaaaaa
     1621 atatgactgc ccccgcggca aacgaggatt aaaatatatg cgtttagtta tggtcaatgc
     1681 gccggaaggc agaggcaaag acatcgctca aatagcgttt tcggcggata tttcaaaggt
     1741 ttctcatcgt caggtgacca gccatcacgc cgacggcaaa accgagacga aagacgtggt
     1801 cgacgtcgaa acctcgacgc cgaaggcaaa gcgttatatt gacggcgttc tggcggcgga
     1861 tttttacaac cccgaagatg tcaccatttc cgtccggcag ccgcgcgcga ttatttcgaa
     1921 agacgatttg cgcgagctga ccaaaccgct ggtcgaaccg gcgaccgata tttttgagga
     1981 actctggcaa ttcaaccata taacttacgg ctttgtcggg cgcatttttc tcgccgcctg
     2041 tctgctggct tacggcttga ttcagcagca gactttgctg attatcgccg gacttttgtt
     2101 tttgccgctg ctgccgctgc tgcttgccgt cggtttcggt tcgtggacgc gcaactggaa
     2161 actttcaatt cagggcgttc tggcttttct gacggcgacg attttgctta tggcaggcgg
     2221 cgcggcgatt gcagccgttt ccgacccgcc gcttaaatac agtgagttca attcgatttt
     2281 agtcggtttt ctgatttcgg tggcggtcgg catcgccgcc ggactggcga atatcgacga
     2341 cgtcggcaga cgcgaaatga tcgggctggc ggcgaccgcg caaatcgcca ttctgcccgt
     2401 ctggttcggc atctgtctgg tcttcggttt tccggcgacg atgagcgaaa gcgaaatcac
     2461 caatcgcgcg ctttcgtttc ttatcaatgt cccgaccatt atcgtcgttt cgctggcggt
     2521 ttatgtttta acgggcgcgg cgagccgttt tctggcgcgg ctcaaggata gataatttta
     2581 aatgctgact cttattgaaa acggagaaat ctactcgccc gaaccgatgg gcaaaggctc
     2641 gattctgctg gttgaaaatc agattctgaa agtcggcgag attaaccgcc gcgccgtcga
     2701 atcgctgggc gtcgaatttg aaatcatcga cgcgcgcaat tgtttaatct cgcccggttt
     2761 tatcgacccg cacctgcatc ttttgggcgg cagcggcgaa aaaggctttt cgacccagac
     2821 gcccgaaata agcgcgaccg aaatcatcgg cgcgggaatc acgacggtcg tcggctgtct
     2881 cggcgtcgat acgacgatga aaacgatgcc gggactgctg gcgaaagcca aagcgctcaa
     2941 agaagaaggc ttgaacgctt tcatctggac gggcggctat aatgtcccgc cgacgacaat
     3001 cagcaaaagc gcgcgcgacg acataatgtt tatcgaagaa gtcatcggcg cgggcgaaat
     3061 cgccatttcc gacgagcgct cgaccgagcc ggattttcac gagctggcgc acctggcgca
     3121 cgacgcatac gtcggcggaa tgctcagcaa aaaagccggc gtcacgcatt ttcacgtcgg
     3181 agagaccggg aaacgcctgc atcttctgcg cgatttaatc gacaaagacg attgtaagcc
     3241 cgaatggctt tacccgacgc acatcacgcg ctccgaagag ctgatgctcg aagccatcgc
     3301 gctcgccgcg cgcggctgtt ttgtcgatat cgacacggtc gaagaagatt tggagaaatg
     3361 gctgaaattt tacaaggata acggcggcga tatgaaaaag ctgaccgttt cctccgattc
     3421 ttcgattacc agtccggcga cgttttttga acaaattcgc gcgtgcgtcg tcgggcaaaa
     3481 attcacgctc gaagaagttc tcccgctcgt tacggcaaac acggcgaaag ctttaaaact
     3541 cgatgcaaaa ggcacaatcg aaatcggcaa atccgccgat tgtctggttc tggaaaaaga
     3601 ttccttcgag ctacgcgaag ttatcgccgg cggtcggcgg ctgttcaaag acggcaaaat
     3661 cgcctttaag gaagcctttc tcaaagacag caaccgcagc atcaatctga ccggcgagaa
     3721 aaaaccgaaa agggcgaaag caagcaaggc aaaaggcacg aggcaaaagt aaagatatct
     3781 aaatcttatt tttacctttg tgccttgtgc cctgaaagcg gttacgccgt ttttgccgtt
     3841 tccgcgcgca tctgcatcca gcgcttcatc atttcgcgcc gcgcggcgag catctgcttc
     3901 gcgtccggca ttttcataat cagcgtttcc agatttttca gataattcgg gcttcccata
     3961 atctgccgca tctcttcgag aaacggctcg accttcgaga aaatcaagac gcattcgccg
     4021 tttgattcga gaaacatttc ctcgtcaatc gcgccgtgat tgacaaagct cgccgccata
     4081 tcccagtaac tggtgaccat ccgaaaatac gcgctcgttt ccgcgctcac catcacctgc
     4141 ataatgtccg ccgcgctttc cggaaaaaac gaagttatcc agcttcgcgc ctcgcgcatc
     4201 ttttcctcgc gccgcaaatc atacaatttc agaatcaaat ccgccgattc agctttgttc
     4261 atcttattgc tcctttttgt atatttttat atttagaatt gcctgcccag tttaattatt
     4321 ttgtatgcgc aggacaaacc aacagaccgg atttcaactg c
//
LOCUS       DASWNE010000760         2526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_517910, whole genome shotgun sequence.
ACCESSION   DASWNE010000760 DASWNE010000000
VERSION     DASWNE010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517910"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(288..1760)
                     /locus_tag="VGB68_14840"
     CDS             complement(288..1760)
                     /locus_tag="VGB68_14840"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEY0050566.1"
                     /translation="MKIKFPLLVVLLLLCFPAIFTIDVRAQDIPFFTNDFPPQEFAAR
                     RAKIFDALGENGLALIQGAPSPAGYVRFRQTNEFYYLCGIESPHAYLLLDGRQRRASV
                     YLPHRNAARERGEGKVLSAEDAELVRQLSGIDNVFAADVLSEHLARYARSGQEMKLFT
                     PFSPSEGVATSRDSAIRAVADIASDSWDGSPSREGHFINLVRTRFPQFEVRDLTPILD
                     RLRLIKSPREIALIKKATRLSGLSLMEAMRSTVPGIQELELDAVAKYIYYRNGAQGDA
                     YYSLIASAGNAWYPHYNAGKRVMRDGDFLLMDYAPDVGYYMSDVTRMFPVNGKFNSWQ
                     RELYGFYLDCYKAILKNIKPGLTARQVKQAAVKEMNQLLAKSRFSKPVYEKAAREFVE
                     NYADSAKNERTSLGHWIGMATHDVGGNDGSPLREGMVFSIEPALRVEEEKIYIRLEDV
                     IVITKTGADILTTFVPAEIDEIEKLMSEDGMLQRYPKDKN"
     gene            complement(1918..2499)
                     /locus_tag="VGB68_14845"
     CDS             complement(1918..2499)
                     /locus_tag="VGB68_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY0050567.1"
                     /translation="MKYKKFDYLFFIIVVLLLMLLRASVRAQTGAGSELQSSGGTFVL
                     TKSAIAGGGKQKQLAPFNENGTSGQPIAGTRSSGGNFSLYSGFWTQESFAPTAATAVA
                     GGRILTADGRGIRGVIVTILFPGGETQRAISSNYGYYRFAEIPAGETYVFTVSAKRHA
                     FSQPSQARQIQEDTQDIDFIADASEPLSSSPLQ"
BASE COUNT          611 a          670 c          617 g          628 t
ORIGIN      
        1 ctataattaa actgatattt ttgcctggct ttaacatatt tttttctccg aaaagaacat
       61 ctaagttaaa atcgcagaaa aaaataataa aacggctcaa ggtttttcgc gctcgtcagg
      121 tttttataat ttctcttgta aaacgcgcgg cgagcccgga aggggcagct atttactcgc
      181 agaaagattt attgtcgacg cccgcctcgg aattttgacg ttttatgatt atcctgcccg
      241 gaagaatatg attatccgtc gggttcctga ttaaggcttt cttatcttca atttttgtct
      301 ttcggatagc gttgcagcat tccgtcttca ctcattagtt tttcgatttc gtcaatctcc
      361 gccggaacga aagtcgttaa tatatcggcg cccgtttttg tgatgacaat cacgtcttcg
      421 agccggatat aaattttttc ttcttcgacg cgaagcgccg gttcaatcga aaaaaccatt
      481 ccttcccgga gcgggctgcc gtcattaccg ccgacatcgt gtgtcgccat accaatccag
      541 tgtcccagcg aagtgcgctc gtttttcgcc gagtcggcgt aattttcgac aaattcacgc
      601 gccgcttttt cataaacggg tttggaaaaa cgtgattttg cgagcaattg attcatttct
      661 ttgaccgccg cctgtttgac ctgccgcgcc gtcagcccgg gcttgatatt tttaagaatc
      721 gctttgtagc agtccagata aaagccgtac agctcgcgct gccaactgtt gaacttcccg
      781 ttgacgggaa acatacgggt gacgtcgctc atgtaatagc cgacgtccgg cgcgtaatcc
      841 atcagcaaaa aatcgccgtc gcgcatcacg cgtttacccg cattgtaatg cggataccag
      901 gcgttgcccg cgctggcaat cagcgaataa taagcgtctc cctgcgcgcc gttacggtaa
      961 tagatatatt tggcgacggc atcgagttcc aactcctgaa ttccgggcac ggtcgagcgc
     1021 atcgcttcca tcagcgagag tcccgaaagc cgcgtcgctt ttttaatcag cgcaatctcg
     1081 cgcgggcttt taatcagccg caggcggtcg agtatcggcg tcaaatccct gacctcaaac
     1141 tgcggaaatc gcgtcctgac gaggttaatg aaatgtcctt cccgcgaagg acttccgtcc
     1201 caggaatccg aagcgatgtc ggctaccgcg cgaatcgcgc tgtcgcggct ggtcgccacg
     1261 ccttcggacg ggctgaaagg cgtgaaaagt ttcatctcct gaccgcttcg cgcgtaacgc
     1321 gccaaatgtt cgctcaaaac gtcggcggcg aatacattat cgatgcccga aagctgtcga
     1381 acgagctcgg cgtcttccgc cgacaggact tttccctcgc ctctttcgcg cgctgcattg
     1441 cggtgcggaa gataaacgga agcccggcgc tgacgcccgt caagcagcag ataagcgtgc
     1501 ggcgactcga tgccgcacag ataataaaat tcgtttgtct gccggaatcg gacgtatccg
     1561 gcaggactcg gcgcgccctg aatcaaagcc agaccgtttt cgccgagcgc gtcgaatatt
     1621 ttggcgcgcc gcgcggcaaa ttcctgcggc ggaaagtcgt ttgtaaaaaa cggtatatcc
     1681 tgcgcccgaa catcaatcgt aaaaatcgcc ggaaagcata acagcagcag cacaaccaaa
     1741 agaggaaact tgattttcat gcagatagat taaacgcttg aaaatatttc cgcaaatgaa
     1801 tttgataatt aaaatgagaa agcgaaagaa tcaaatccga agatgcttcc ggctcggaag
     1861 aatcatttcc gaaacggaat ttaccggaaa atttctactc tttacattta ataattacta
     1921 ctgtagcggc gagcttgaaa gcggctccga cgcatcggcg ataaaatcga tatcctgcgt
     1981 gtcctcctga atttgtctgg cttgcgacgg ctggctgaaa gcgtgacgtt tggcagaaac
     2041 ggtgaaaacg tatgtttcgc ccgccggaat ttcggcgaag cgataataac cgtaattact
     2101 cgaaatcgcc ctttgcgttt cgccgcccgg aaagaggatt gttactataa cgccgcgtat
     2161 tcctcttccg tcggcggtca aaatacgacc gccggcaaca gcggtcgccg ctgtcggcgc
     2221 gaagctttcc tgagtccaga agccggaata aagagagaaa ttaccgccgc tcgaacgcgt
     2281 tccggcaatc ggctgcccgc tcgtgccgtt ttcgttaaaa ggcgcgagct gtttctgctt
     2341 tccgccgccg gcgatggcgg atttcgtcag cacaaacgtt ccgcccgatg attgcagttc
     2401 actgcccgcg ccggtttgcg ctctaacgga cgctctcaaa agcatcagca gaagcacgac
     2461 gataataaaa aaaaggtagt cgaatttttt atatttcatt gttttagctc ctttaaccgg
     2521 acgccg
//
LOCUS       DASWNE010000761         6352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_694975, whole genome shotgun sequence.
ACCESSION   DASWNE010000761 DASWNE010000000
VERSION     DASWNE010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6352
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694975"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VGB68_14850"
     CDS             <1..188
                     /locus_tag="VGB68_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050568.1"
                     /translation="YLAVNDALKLPNIGAAIPLAYMGFMAFLVLSAILIIPSFMVGLL
                     TGKIQQVIFSKLIVTYH"
     gene            589..>6352
                     /locus_tag="VGB68_14855"
     CDS             589..>6352
                     /locus_tag="VGB68_14855"
                     /inference="COORDINATES: protein motif:HMM:NF014503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0050569.1"
                     /translation="MKNSALKVCLFVSLLAVSAGFNLSYAKIGGSSSGKKNSLKASSE
                     NVDKSKDSTKAEGSQAAVKITDLPIAFEENKGQAGSGVKFLSRSRNYNLFLNADGSVL
                     QFRDTECEAKRKLDKDSRGKTMISASKPCKIYRLGMKMLGINGEAAIQGTDELIAKSN
                     YIIGNDSSKWQTGITNFQGVFYRGVYDGIDLVFRGAEQKLEYDFHVAPGADPNLIRLE
                     FDGTKKIKIDRDGDLVFKFKGMEIRHQQPFAYQIIDGQKKQISARFISLGKNKIGFIV
                     GDYDKTKELVIDPIIYATYLGGSRQDYTEDITVDSAGYIYLASDAQSENFLDPNGTII
                     PGVANDKDIFVTKLSPDGTQIIFNTVVGGNNSDIPWAIDIDGFQNIYVGGQTSSTNFP
                     MVNARQTSHSIADSSDVEGYVFKLNASGNSLAYSTYHGATSNANGYHTDWVESVNVDS
                     SGNLYAVGLSGGTNFPLLNAYSTSIRGNFSAYLSKLSPSGQLLYSTFFTGIESFCWTA
                     AYDVDVDDNGFAYISGEVAGAGHVPTTPGAYKTSGTGFIAKFDTEKTGSGSLVYATHS
                     PSIGEAIGIDSYGNAAVVDSGSRFRIGNGVKINSAGTAAVFTFTTRGSAGDLAINAAG
                     NIYYTEDIDDPTNPSRTDVLVKGLSPTGALLGTEVLHATASDRATGIAIGLDNLIYVV
                     GYTGSLDFPTTSGALQPNSRYAIPGGSSLQGFFAKVRLDPPEPLEPLIFIPGVAGSDL
                     YEADADGNPKPVSIDTPVPRIWLSGAVTDIFGFSLLNKLDLTPPASSHPNIVAVDVLR
                     DELLGAYKVYGPLFRKLEEVGYVEYDIQKNPKRRNVQCDPSWTDVNGKKPTLFVFAYD
                     WRLSNAEAAQKLSAYIDCVRSFYSDKKVNILTHSMGALVARRYILDHQNEQKVKRLVT
                     LVAPFLGAPKAIDALFTGRFVGKAGYYAHKDAIKKVVSYAPGAHELLPSEWYYDLESG
                     ADKTPFAYKSPYLQLTTDYNYAQAKNYLNNYFPSKPYDNNQSFHNYSNNAKLQDNWSS
                     DSSGIKYYHIYGVERGESTIGKVIHAPYIVFPQSDEQRDYGVELEYTKGDGTVPLLSA
                     KRARENLNPILLAPNTKMKQCNAPFTIFNSGYDHNGVLTNPVIWEELLAMLEDRNINQ
                     PECVTNFSNSPLTEEGGGEGNTAENGGGGESNETSGGQQITNDASLNYLQVYKVNRLQ
                     ITDESGNTNTQLNPDADKAVPDIDYEYGSSAVDSLVEPHEVSFAAGKKVDIKFVAPAN
                     RVKIVNSVGTDRNNPAQSVKYMDLQLPAGATAWLKFTETGVENLRLDANNDGTFETEI
                     QPTFTLTGAAATDKTPPTVSISYTVNNNVATVTASAADNETGINQIRYIINSENIDRL
                     YNSPFTIDLTQTQLIYVCAEDNAGNRSLLAKWLDLAVPNTSVNQTPPANSAGWNTSDI
                     TLELKAVDDIGGAGVESLTYGGSGAQPFSQQTLAQPDAPLTYPKPNTATDFLAKTFNF
                     YTEGVTTLTFFAKDKAGNVEATKTHFVKIDKSDPISQHTLVDNGQQTTFTLNAADAVS
                     GVSEIVYSIDGAAAQTYTAPFSVSNTGGHYVTFYARDLAGNEEAPHTIYINAAQVSKS
                     VLVSEFRTRGSLGADDEFIELYNSGDTNVDISGWVVSIKQGSVSTPTTLATINSGAVI
                     PARGHYLLRKAGASNYSLSAYAEADQTYSGAMGDNVGIALFSTASIIIDAVGFSSVTD
                     TTYREGTALAPAAGISTSGQYSFVRNYNSRGFPVDSNSNRADFIFVATDGATYNNLKA
                     VLGSPGPENLSSPVYRSGAILVNQVDDKVTLAESPNRVYDYTATSQQYPVGILSLRRK
                     LTNNTGSTVTGLRLRITGLTTRNSAVIFPQQAIIRLINSADISVTSGNGQTTIPLKGL
                     TIDAIPTTVDGGFNTSLTLNL"
BASE COUNT         1696 a         1644 c         1557 g         1455 t
ORIGIN      
        1 gctatctcgc cgtcaatgac gctttgaagc ttcccaatat cggcgcggct attccgctcg
       61 cttatatggg atttatggcg tttttggtgt tgtctgcgat actcataatc ccgtccttta
      121 tggtcggttt gctgaccgga aaaatacagc aggtcatttt ttcaaaacta atcgtgacct
      181 atcactaata tttttcttca attccggcag gcgagcgcac gggctgtttt ttatataaaa
      241 tttcaattgc aaaacctgcg aaaatccgtt ccgccgaatt tctaattccg cgcggaaaaa
      301 aaacgaaagt ttcttgacat aatcgccgcg ctgggctaat cttccttcgt tcttctttac
      361 acaggtaaaa tcttagcgtt ttctaatcgc gaaaattcgt tgtctaacaa ccgggttgga
      421 aaactacgat tctattttga gttctccctg cttcggtttt gaggaacgtg attcttccac
      481 attcagcccg gttaagactt ttccctaagc tgctgcgttg ttgagttggc aataatcact
      541 ttgtcagaaa tttagctcta tccaaatata tttccttgag ataaaaaaat gaaaaattct
      601 gcactcaaag tgtgtctgtt cgtgtctctg ttagccgtct cggctggttt taacttaagt
      661 tacgcaaaaa tcggcggcag ctcttccgga aaaaaaaaca gtcttaaagc ttcttcggaa
      721 aacgtcgata aatcaaaaga ttcaactaaa gccgaaggct cgcaagcggc ggttaaaata
      781 accgatttgc cgattgcttt tgaagaaaac aaaggtcaag ccggcagcgg cgtcaaattt
      841 ctgtcgcgca gccgaaacta taatctgttt ctcaacgccg acggaagcgt ccttcagttt
      901 cgagatacgg aatgtgaagc aaaaaggaaa ctcgataaag acagtcgcgg aaaaacgatg
      961 atttccgctt ccaagccctg caaaatttac aggctgggca tgaaaatgct cggcattaac
     1021 ggcgaagccg caattcaggg cacggacgag ctcatcgcca aaagcaatta tataatcggc
     1081 aatgattcga gcaagtggca gaccggaatc accaattttc agggcgtttt ctaccgcggc
     1141 gtctacgacg gcatcgacct cgtttttcgc ggcgcggagc aaaagctgga atatgatttt
     1201 catgtcgcgc ccggagccga cccgaatttg attcgactcg aatttgacgg gacaaaaaaa
     1261 ataaaaattg accgcgacgg cgatttagtc ttcaaattta aaggcatgga aatccggcat
     1321 caacagccgt ttgcctatca aataatcgac gggcagaaaa agcaaatttc cgccagattt
     1381 atttcgctcg gaaaaaataa aatcggattt atcgtcggcg attacgataa aacaaaagag
     1441 ctggtcatcg acccgattat ttacgccact tatctcggcg gaagccgtca ggattacacc
     1501 gaggacatta cggtagattc cgccggttat atctatctgg cgtcggacgc ccaatcggaa
     1561 aattttcttg accccaacgg gacgattatt ccgggcgtcg ccaatgacaa ggatattttt
     1621 gtcaccaaac tcagcccgga cggcacgcag ataattttca acaccgtcgt cggcggcaac
     1681 aatagcgata ttccgtgggc gatcgatatt gacgggtttc agaatattta cgtcggcgga
     1741 caaacttcct cgacgaattt cccgatggtc aacgcgcggc agacttcgca cagtatagcc
     1801 gactcgtccg acgtcgaagg ctatgtgttc aagctgaacg cttcgggaaa ctcactcgcc
     1861 tacagtactt atcacggcgc gacttcaaac gccaacggct atcacaccga ttgggtagaa
     1921 tcggtcaatg tcgattcgag cggaaatttg tatgccgtcg gcttgagcgg cgggacgaat
     1981 ttccctttgc ttaacgctta cagcacgtcc atcaggggca acttcagcgc gtatctttca
     2041 aaactcagcc cgtcgggaca attgctttat tcaacctttt tcaccggcat agaatccttt
     2101 tgctggacgg ctgcctacga cgtcgatgtc gacgataacg gatttgcata catttccggc
     2161 gaggtcgcgg gcgcaggtca tgtgccgacc acgcccggcg cttataaaac ctccggcacg
     2221 ggatttatcg ccaagtttga caccgaaaaa acgggtagcg ggtcattggt ttacgccacg
     2281 cattcgcctt ctatcggcga agcgataggc atcgacagtt acggaaatgc ggcggtcgtc
     2341 gacagcggaa gccgatttag aatcggcaac ggagtcaaaa tcaacagcgc cggaaccgcc
     2401 gccgtcttta cctttacgac gcggggcagc gccggcgacc tcgccatcaa cgccgccgga
     2461 aatatatatt acacggaaga catcgacgac ccgacgaatc cttccagaac cgacgttctg
     2521 gtcaaaggct taagtccgac gggagctttg ctgggaactg aagtgctgca cgctactgcg
     2581 agcgaccggg cgacgggcat tgccatcggt ttggataatt tgatttatgt ggtcggctac
     2641 acgggttccc tggattttcc gaccaccagc ggcgcattgc agccgaacag ccgatacgcc
     2701 attcccggcg gcagctcgct gcaaggattt tttgccaaag tcaggctcga tccgcccgag
     2761 cctctagagc cgttaatttt cattcccgga gtagccggaa gcgaccttta tgaagcggat
     2821 gccgacggaa atccgaaacc ggtttcgatt gacactccgg ttcccagaat atggctttcc
     2881 ggtgcggtga cggatatctt tggcttctcg ctgttaaata aattggattt aacaccgccg
     2941 gctagctcgc atccgaacat cgtcgccgta gatgttttac gagacgagct tctcggagct
     3001 tataaagttt acggtccctt attcaggaaa ttggaagaag tcggatacgt agaatacgat
     3061 attcagaaaa atccgaagcg ccgcaacgtg caatgcgacc caagctggac ggatgtaaac
     3121 ggtaaaaaac ccacgctttt cgtttttgct tacgactgga gactctcaaa cgccgaagcc
     3181 gcgcagaaat tgtctgccta tatcgattgc gttcgctcat tttacagcga caaaaaagta
     3241 aatattctga ctcacagcat gggcgcgctg gttgccagac gttatattct cgaccatcag
     3301 aacgaacaga aggtcaaaag actggtgacg ctggtcgctc cgtttttggg cgcgccgaag
     3361 gcgatagacg ctctgtttac agggcgattt gtaggcaaag cgggttatta cgctcataag
     3421 gacgctatca aaaaggtcgt ttcatatgcg cccggcgctc acgaattgct tccgagcgaa
     3481 tggtattacg atcttgaatc gggcgcggac aaaactccgt ttgcctataa atctccttat
     3541 ttgcagctga ctacggatta taattacgca caggcgaaga attatctaaa caattatttc
     3601 ccgagcaagc cttacgataa taatcagtct tttcacaact attcaaataa cgccaagctt
     3661 caggacaatt ggtcttccga ttcgagcggc atcaagtatt accacatcta cggagtggaa
     3721 agaggagaaa gcaccatcgg taaagttatt cacgctccat atatcgtttt tccgcaatcc
     3781 gacgaacaaa gggattacgg agtggagctg gaatatacca aaggagatgg aaccgtgccg
     3841 ctcttaagcg cgaaacgcgc gcgggaaaat ttaaatccca tactgctcgc gccgaatacg
     3901 aagatgaagc aatgcaatgc gcctttcact attttcaata gcggctacga ccataacgga
     3961 gtgctgacaa atccggtcat ctgggaagaa ctattggcga tgctcgaaga ccgaaatatt
     4021 aatcagccgg aatgcgtcac caatttttcc aattctccgc tgactgaaga aggcggcgga
     4081 gaaggcaaca ccgcagaaaa cggcggcgga ggggaaagca acgagacatc gggcggacag
     4141 caaataacga atgatgcgag cctgaattat ttacaggttt acaaagtcaa tcgtctgcaa
     4201 attacggatg aatcgggaaa tacaaacact cagctcaacc ccgacgcgga caaagccgtg
     4261 ccggatatag attatgaata cggctcaagc gccgtcgatt ccctggtcga accgcacgaa
     4321 gtatcttttg ccgcaggcaa aaaagtcgat ataaaattcg tcgcgcccgc caatcgggtt
     4381 aaaatcgtaa attccgtcgg aaccgaccgc aacaatccgg cgcaatcggt taaatatatg
     4441 gatttgcagt tgcccgcagg cgcgacggcg tggctgaaat ttacggaaac cggagtcgaa
     4501 aatctgcgcc tcgacgctaa caatgacgga acttttgaaa ccgagataca accgacgttt
     4561 actttaacgg gcgctgccgc gactgacaaa acgccgccga ccgtcagtat ttcatacacc
     4621 gttaataata atgtggcaac cgtcaccgcc agcgccgccg ataacgagac gggcattaac
     4681 cagattcgtt atattattaa cagcgagaac atcgaccgcc tttacaattc gcctttcacg
     4741 attgacctga cgcaaacgca gctcatatac gtctgcgccg aggataatgc cggaaaccgc
     4801 agcttgctgg ctaaatggct ggatttagcc gtcccgaaca cttccgtcaa tcaaacgccg
     4861 cccgccaaca gcgcgggctg gaatacgagc gacatcacac ttgagctgaa agccgtcgat
     4921 gatattggcg gagcgggcgt cgaatccctg acttacggcg gaagcggcgc gcagccgttt
     4981 tcgcagcaga cattggcgca gccggatgcg ccgctcacat atccgaagcc gaataccgcc
     5041 actgattttc tggcgaaaac tttcaatttc tacaccgaag gcgtcacgac cctaactttc
     5101 tttgccaaag ataaagccgg aaacgtcgaa gcgacgaaaa cgcacttcgt caaaatcgac
     5161 aagagcgacc cgattagcca gcacacgctg gtcgataacg ggcaacagac cacttttacc
     5221 ttaaatgctg ccgacgccgt cagcggagtc agcgagattg tttactcaat cgacggcgcc
     5281 gccgcgcaaa cttataccgc tcctttctcc gtttctaata cgggcgggca ttacgtgacc
     5341 ttctacgcca gggatttagc cggaaatgaa gaggctcccc acacaatcta cattaacgcg
     5401 gcgcaagttt cgaagagcgt gctcgtcagc gaattcagaa cgcgcggctc gctcggcgcg
     5461 gacgatgaat ttatcgaact atataacagc ggcgacacta atgtggacat cagcggctgg
     5521 gttgtcagca tcaagcaagg cagcgtttcc acaccgacga cgctggcgac tatcaacagc
     5581 ggcgcggtga taccggcgcg cggacattat cttttaagaa aagcgggcgc gagcaattac
     5641 agtctttccg cttatgccga agccgaccag acatattccg gcgcaatggg cgacaatgtc
     5701 ggcatcgctc tgttttccac ggcatcgata attatcgacg ccgtcggatt ttcgagcgtc
     5761 accgacacta cctatagaga aggaacggcc ctcgctccag ccgccggaat ttcgaccagc
     5821 ggtcagtata gcttcgtcag aaattataac tcccggggtt ttccggtcga ttccaacagc
     5881 aaccgcgcag attttatatt cgtcgcaact gatggcgcga cctataataa cctgaaagcc
     5941 gttctcggct cgcccggacc agaaaatcta agcagtccgg tttatcggag cggggctatt
     6001 ctggtaaatc aggtcgatga caaggtaact ctggcggagt cgccgaaccg ggtatacgat
     6061 tacaccgcga cgagccagca atatccggtc ggaatcttgt cgctgcgccg gaagctgacg
     6121 aataatacgg gcagcaccgt aaccggtctg aggttgagaa ttaccgggct gacaaccagg
     6181 aacagcgcgg tcatctttcc gcagcaggcg attatccggc tcatcaactc cgcggatatt
     6241 tcggtgacca gcggtaacgg gcagacgacg attccgttga agggcttaac cattgacgcg
     6301 attccaacca ccgtcgatgg cggcttcaat accagtttga cgcttaattt ag
//
LOCUS       DASWNE010000762         1525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_273583, whole genome shotgun sequence.
ACCESSION   DASWNE010000762 DASWNE010000000
VERSION     DASWNE010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273583"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VGB68_14860"
     CDS             <1..223
                     /locus_tag="VGB68_14860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050570.1"
                     /translation="GGQGISKARVFIAAAADETVQASATGNFGYYRFNDISAGQTYIL
                     GASHKSVNFSPQAVTVLEEVENINFVALP"
     gene            complement(393..>1525)
                     /locus_tag="VGB68_14865"
     CDS             complement(393..>1525)
                     /locus_tag="VGB68_14865"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY0050571.1"
                     /translation="VDEQNRTIYFLAGGRERGRNPYFSHFYKVGFDGKNLQLLTPEDG
                     NHSITLSPSGRYFVDSFSKQDTPPVIVLRDAEGKLISNLEKADISRLTAAGWKPPTPI
                     VVKARDNQTDLYGVMYTPSNLDPNRKYPIINYIYPGPQGGSVATWSFAHAVSDHQSLA
                     ELGFIVVAIEGTCNPLRSKKFHDACYGNMADNTLPDQITAMQQLARKYPFIDLNRTGI
                     WGHSGGGFATAAAMFRYPDFFKVGISESGNHDSRNYEDDWGERYIGLLTPTADGKSSN
                     YEAQANQIYAKNLKGKLLLAHGMMDDNVPPYNTLLVVDALVKANKDFDLIMFPQARHG
                     FGSASLYMTRRRWDYFVRHLLGVNPPTEYELKSTDDPRNTLP"
BASE COUNT          329 a          403 c          414 g          379 t
ORIGIN      
        1 cggcgggcag ggaatttcga aagcccgcgt tttcattgcg gcggcggcgg acgaaacggt
       61 tcaagcgtcg gcgaccggta acttcggtta ttaccgtttt aacgatattt cagccggtca
      121 gacatatatt ctcggcgctt cgcataaatc cgtaaatttc tcgccgcagg cggtcaccgt
      181 tttggaggaa gtggaaaata tcaacttcgt cgcgcttccc tgaaacaatt ctcggagttt
      241 aacttgatta caaaaatcat tcgggctcgg ttttacactt ggaaaccgca gcttttaagt
      301 gtaaaaccgg agtttttaat ctcgaaggcg gaaaccgcgc cctatagctt ttaaatcttc
      361 cgaagatatt ccgcctgttt tatttccaac ctttacggca gagtattgcg cgggtcgtcc
      421 gtggatttca gctcgtattc cgtcggcgga ttgacgccca acaggtggcg cacgaaataa
      481 tcccagcggc ggcgcgtcat atacaggctc gcgctgccga agccgtgacg ggcttgcgga
      541 aacattatca aatcaaaatc cttgtttgcc ttaacaaggg cgtcgacgac gagcagagtg
      601 ttatacggcg gcacattgtc gtccatcatt ccgtgcgcga gcagcagttt tcctttcaga
      661 tttttggcgt aaatctgatt cgcctgcgct tcgtaattcg acgatttgcc gtccgccgtc
      721 ggcgtcaata aaccgatata tctttcgccc caatcgtctt cgtaattgcg gctgtcgtga
      781 ttgcccgatt cggaaatgcc gactttgaag aaatcgggat agcgaaacat cgccgccgcc
      841 gtcgcaaaac cgccgccgga atgtccccag atgcccgtcc ggttcaggtc gataaacgga
      901 tatttccggg cgagctgctg cattgcggtt atctggtcgg gcagcgtgtt gtcagccata
      961 ttgccgtaac aggcgtcgtg aaatttcttc gagcgcagcg ggttgcacgt tccttcgatg
     1021 gcgacgacga tgaagcccaa ttcggcgagc gattgatggt cgctgacggc gtgcgcgaaa
     1081 gaccaggtgg cgacgctgcc gccctgcgga cccggataaa tgtaattgat aatcggatat
     1141 ttccggttcg ggtcgagatt cgagggcgta tacattacgc cgtaaaggtc tgtctggttg
     1201 tcgcgcgcct tgacgacaat cggcgtcggc ggtttccagc cagccgccgt caggcgcgaa
     1261 atatcggctt tctcaagatt tgaaatcagc tttccttccg cgtcgcgcag gacgataacc
     1321 ggcggcgtgt cctgtttcga aaaactgtcg acgaaatagc gccccgacgg cgacagcgtg
     1381 attgaatgat ttccgtcttc cggcgtcagc agttgaagat ttttcccgtc aaagccgact
     1441 ttataaaaat ggctgaaata cgggttgcgc ccgcgctcgc gcccgcccgc gagaaaataa
     1501 atcgtccggt tttgctcgtc gactt
//
LOCUS       DASWNE010000763         1586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_129779, whole genome shotgun sequence.
ACCESSION   DASWNE010000763 DASWNE010000000
VERSION     DASWNE010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129779"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            356..1255
                     /gene="cyoE"
                     /locus_tag="VGB68_14870"
     CDS             356..1255
                     /gene="cyoE"
                     /locus_tag="VGB68_14870"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEY0050572.1"
                     /translation="MENIATEIQEAKAFNAREKASAYFELTKPRIAFMLVLTAAAGFY
                     LGSDMGFDWLLFLNSMLGITFLAFGVATLNQFIERNIDALMERTARRPLPSGKLNGAE
                     ALIFGILLCLGAEIYLVFLVNGLTAILGLIVIVGYVLLYTPLKTRTSASTAIGALPGA
                     MPPLMGWTAAAGEITLGAWILFAMLFLWQFPHFLAIAWMYKEQYKKAGILMLPVVEPE
                     GKITARQIVLFTVLLLPVSLAPYFIGLAGLIYLIGAIILGGWFLYSSIQAARAKSIPQ
                     ARKLLLVSVLYLPLIFALMVFNH"
     gene            1279..>1586
                     /locus_tag="VGB68_14875"
     CDS             1279..>1586
                     /locus_tag="VGB68_14875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050573.1"
                     /translation="MSLEVGTAETTIDVEPEKKRKRSSLGGGGNPGNGGRKRGGGGGG
                     DDGDNRNKDRSFEEIEEFRPDKWRVAMWFTLLVVVMTFGGLISAYIVLSTNQDLEWKP
                     "
BASE COUNT          361 a          357 c          418 g          450 t
ORIGIN      
        1 tgcggcgctt ccttgacgac agtttcttct aaaacggaca taaagtgcga acgtcggaat
       61 ttaaaacgca aaattcaaat gttttgaata ctctaattaa taagtcgggt tttgcgcttt
      121 atagtctaaa aaaaatcata acttatgcgg gttttattgt catcccgcta aaaattagtt
      181 tctcttttgt gaaaaagaag ttttgttttg cctgttgcag tttggagaat tgcaaaggcg
      241 aggttattct tgaaaatatc cgacctttga agtttgaact tagactttta tcaagtcgat
      301 tttcaaaggt cttaagttca tcaaaggtct taagttcaaa ggtcaaaaat ctgtaatgga
      361 aaatatcgca actgaaattc aggaagccaa agcctttaac gcgcgcgaaa aagcctcggc
      421 ttattttgag ctgaccaagc cgcgcatcgc gtttatgctg gttttgaccg ccgccgccgg
      481 attttatctc ggctccgata tgggcttcga ctggcttttg tttctcaatt cgatgctcgg
      541 catcacgttt ctggcgttcg gcgtggcgac gctcaaccag tttatcgaaa gaaacatcga
      601 cgcgctgatg gaaagaacgg cgcggcgtcc gcttccgtcg ggaaaattaa acggcgccga
      661 agctttgatt ttcggcattc tgctttgtct gggagcggaa atttatctcg tttttctggt
      721 caacggcttg acggctatcc tgggtttaat cgttatagtc ggctacgttt tgctttacac
      781 gcctttgaaa acgcgcacct cggcttcgac ggcgatcggc gcgctgccgg gcgcgatgcc
      841 gcccttgatg ggctggactg ccgccgccgg cgagatcacg ctcggcgcgt ggattttgtt
      901 tgcgatgctt tttctgtggc agtttccgca ttttctggcg attgcgtgga tgtataaaga
      961 gcaatacaaa aaagccggaa ttttgatgct gccggtcgtc gagccggaag ggaagataac
     1021 ggcgcgccaa atcgtgctgt tcaccgtttt gcttttgccg gtcagcctcg cgccgtattt
     1081 tatcggtctg gcgggtctga tttatttaat cggagcgata attttaggcg gctggttttt
     1141 gtattcttcg attcaggcgg cgcgcgccaa atccattccg caggcgcgaa aactgctgct
     1201 cgtttcggtt ttatatttac ctttgatttt tgccttgatg gtttttaatc attagcattc
     1261 agctctcgct tttgttttat gagtttagaa gtcgggacag ccgaaaccac aattgatgtc
     1321 gagccggaaa aaaaacgaaa gcgctcaagc ctgggcggcg gcggaaatcc gggaaacggc
     1381 gggcgcaagc gcggcggcgg cggtggcggc gacgacggcg acaatcgaaa caaagaccgg
     1441 agttttgagg aaatcgaaga attccgcccg gacaaatggc gcgtcgcgat gtggtttacg
     1501 ctgctggtcg tcgtgatgac cttcggcggc ttaatcagcg cgtatatcgt cctttcgacc
     1561 aatcaagacc tcgaatggaa gccttt
//
LOCUS       DASWNE010000764         3812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_407521, whole genome shotgun sequence.
ACCESSION   DASWNE010000764 DASWNE010000000
VERSION     DASWNE010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407521"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VGB68_14880"
     CDS             complement(<1..438)
                     /locus_tag="VGB68_14880"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEY0050574.1"
                     /translation="MVDWNSFINLKKPKVFINYRRADAPGHTGRLYSDLARSFGEQNV
                     FMDNSTIRLGEEYKQRIRNEVSACDVFIVVIGKQWLEILSQRQGTEDFVRLEITQALG
                     EKTLIVPVTVENAPVLRAEDLPADLKPITERQTFDIRDGRWSDD"
     gene            complement(637..1566)
                     /locus_tag="VGB68_14885"
     CDS             complement(637..1566)
                     /locus_tag="VGB68_14885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050575.1"
                     /translation="MLQSLKNKLKKSAFIHGTYMRLKRAFAQELVGMTSKTEQDYFAV
                     YGEKTYSGKGEIVDLGCWLGSTTIPLVKGLLKNPAFAESNRKVYAYDLFIWFEWMNES
                     AAGTRLAGKYKEGDNFVEEFKARTKEFARRIEIREGDLKQIGWSGENIEFLLVDAMKN
                     WDLANAVVRDFYPSLVPGESIVLHQDYAHFFTPWIHLLQWTFRESFAFAGEVPKSQSV
                     IFRYENKIAGELLNKNYSFESFSDREVDSAFDYSLNLVSTEKLPNVAAAKVMWFLHQN
                     EREKAKAEFENLLKRGIPLEKDLFVVKEMLEKE"
     gene            complement(1627..1914)
                     /locus_tag="VGB68_14890"
     CDS             complement(1627..1914)
                     /locus_tag="VGB68_14890"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY0050576.1"
                     /translation="MPISSDKRQIQRTNRFSRDIRRLPDNIQKEAFQIAARLSEDIFD
                     SLLNIKPMTGFRGIYRVVVVRDYRMIFSFDDTNIYLLRVGHRREIYRNLEI"
     gene            complement(1895..2101)
                     /locus_tag="VGB68_14895"
     CDS             complement(1895..2101)
                     /locus_tag="VGB68_14895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050577.1"
                     /translation="MKNITLQISEEELREVTQAYKTLQNFLERIISPNELYTDEFLDG
                     LREAQDEVNDKNFVEVKNFADFIR"
     gene            complement(2131..3555)
                     /locus_tag="VGB68_14900"
     CDS             complement(2131..3555)
                     /locus_tag="VGB68_14900"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY0050578.1"
                     /translation="MFQSIHFNAKPRRTTNASKPEVVEKLQKIVGAENVLVEPEKVEP
                     YGADAVKEKFPPEAVVFPETTRQMVEILKLANEYLFPVTARGGGVGYTGGAVPVDGGI
                     VVGTDRMKKIIEISVDDLYVVCQPGLTTYELQQAVEKHGLMFPPDPASYKDSYIGGNI
                     AENAGGMRTPKYGVTKHSVLGLEVVTATGEIIRTGGKTVKNVVGFDLTGLMCGSEGLL
                     GIITEATLKLLPMPEATSTVRANFKTMEEACRVLTKFTPEGLLPMAMEVIDKYCIEAV
                     EGNFAFGLSKEAGAILLIAVDGFREEVEKNAQTIERIIKANGGFDVLRAKNKEEEDKL
                     WDVRRAISPSLMKYGTLKINEDVVVPRSRVPELVARIEEIGRRHDTFVANFGHAGDGN
                     IHVNFLCDRDNPESVRRARRCVKETFALSVELGGSISGEHGIGYVKAAYLDMAIDAPT
                     LAIMKNIKKVFDPNGVLNPGKMFV"
BASE COUNT          906 a          967 c          889 g         1050 t
ORIGIN      
        1 atcgtcactc cagcgcccgt ctcgaatgtc gaacgtctgc cgctcggtta tcggctttaa
       61 gtcagccggc aaatcttcgg ctctcaaaac cggcgcgttt tcaaccgtga ccgggacaat
      121 aagcgttttc tcgccgagcg cctgagttat ttccagccgc acaaaatctt ccgttccctg
      181 tcgctgcgat aatatctcaa gccattgttt accgataact acgataaaaa catcgcaggc
      241 ggaaacttca tttctgattc tttgcttgta ctcttcgcct aaccttatgg tgctgttgtc
      301 cataaagaca ttttgctctc cgaaagacct ggctaaatcc gagtataacc gtccggtatg
      361 tccgggcgcg tcggcacgtc gatagttgat gaataccttc ggtttcttta aatttatgaa
      421 tgaattccag tcaaccatat tcgatgaaag cagattaaac agcgagatgc atattatcac
      481 caaaccatcc gatacgccat aaaaattcca aacgaacggc gcgccggaat ttagaacgcc
      541 gttcggctca aaaactttcc tcgcgccgtt cctgatttcc agcgtccggg tattaattgt
      601 taaagaatgg tgaggaagat ttgccgaacg cgtattttat tctttttcca gcatttcttt
      661 gaccacaaac aaatcttttt ccagcggaat tccgcgtttc agcaaatttt caaattcggc
      721 tttcgccttt tcgcgctcgt tttgatgaag aaaccacatt actttcgccg ccgctacgtt
      781 cggcagtttt tccgtcgata cgagattcag cgaataatcg aaagccgaat ctacttcgcg
      841 gtcggaaaaa gattcgaaag aataattttt gtttaataat tcgccggcga ttttgttttc
      901 gtaccggaaa atcacgctct gcgatttcgg gacttcgccc gcaaacgcga agctttcgcg
      961 aaaagtccat tgcagaagat gtatccacgg cgtgaagaaa tgcgcgtagt cctggtgcag
     1021 aacgatgctc tcgccgggga cgagcgaagg gtaaaaatcc ctgacgacgg cgtttgccaa
     1081 atcccagttt ttcatggcgt cgacgagcag aaactcgata ttttcgccgc tccagccgat
     1141 ttgttttaaa tcgccttcgc ggatttcgat gcggcgggca aattctttcg ttcgagcctt
     1201 gaattcttcg acgaaattgt cgccttcttt atattttccc gcaaggcgcg ttccggcggc
     1261 gctttcgttc atccattcga accagatgaa taaatcgtag gcgtaaactt tccggttcga
     1321 ttcggcaaaa gccggatttt ttaataaacc tttgaccagc ggaatcgtcg tcgagccgag
     1381 ccagcagccc aaatccacga tttcgccctt gcccgaataa gttttttcgc cgtacacggc
     1441 aaaataatcc tgctcggttt tcgaagtcat cccgaccagt tcctgcgcga aagctctttt
     1501 caggcgcata tacgtgccgt gaatgaaagc gctctttttg agtttatttt ttaagctttg
     1561 cagcatccgc ttatattaaa caaacatttt gcccggattc aaaacgccgt tcgggtcgaa
     1621 gacttttcaa atttccaaat tgcgataaat ctcgcgccgg tgcccgacgc gaagtaagta
     1681 aatatttgta tcgtcaaacg agaaaatcat ccggtaatcg cgcacgacga caacgcgata
     1741 aatgccgcga aagccggtca tcggcttgat gttcagcaat gaatcgaaaa tatcttcgga
     1801 aagtcgtgcg gcaatttgaa aggcttcttt ttgaatattg tccggcaggc ggcgaatgtc
     1861 tctgctaaag cgattagtgc gttgaatttg tcgtttatcg gatgaaatcg gcaaaatttt
     1921 tcacctcgac gaagttttta tcattaactt catcttgagc ttcccgcaaa ccgtctaaaa
     1981 attcgtcggt ataaagctca ttcggcgaaa taatcctttc gagaaaattt tgcagagttt
     2041 tataagcctg cgtcacctcg cgcaattctt cttcagatat ttgcagtgta atatttttca
     2101 taacttcccg attatctcac aaaaaagatt tcaaacaaac atttttcccg gattcaaaac
     2161 gccgttcggg tcgaagactt ttttgatgtt tttcataatc gcgagcgtcg gcgcgtcgat
     2221 tgccatatcc aggtaagcgg ctttgacgta gccgatgccg tgctcgccgg agatcgagcc
     2281 gccgagctcg accgagagag cgaaagtttc tttcacgcag cggcgggcgc gccgaacgga
     2341 ttcgggatta tcgcggtcgc acaggaaatt gacgtgaatg ttgccgtcgc cggcgtgtcc
     2401 gaagttggcg acgaacgtgt cgtggcggcg tccgatttcc tcgatgcgcg cgaccaactc
     2461 gggaacgcgc gagcgcggca cgacgacgtc ttcgttgatt ttcagcgtgc cgtatttcat
     2521 cagggacggc gaaatggcgc gccgcacgtc ccagagcttg tcttcttcct ctttattttt
     2581 ggcgcgtaaa acgtcgaagc cgccgttggc tttgataatt cgttcaatcg tctgcgcgtt
     2641 tttttcgact tcctcgcgaa atccgtcgac ggcgatgagc agaatcgcgc ccgcctcttt
     2701 cgagagtccg aaggcgaaat tcccttcgac ggcttcgatg cagtatttgt cgatgacttc
     2761 catcgccatc ggcagcaagc cttcgggcgt gaatttcgtc aaaacccggc aggcttcttc
     2821 catcgttttg aaattggcgc gcaccgtcga agtcgcttcc ggcatcggca gcagcttcag
     2881 agtcgcttcg gtgatgatgc ccaaaagtcc ttccgagccg cacatcaagc cggtcaaatc
     2941 gaaaccgacg acgtttttga cggtttttcc gcccgtgcgg ataatttcgc ccgtcgccgt
     3001 gacgacctcc aagcccagaa ccgaatgttt cgtcacgccg tatttcggcg tgcgcattcc
     3061 gcccgcgttt tcggcgatgt tgccgccgat gtaggaatct ttgtaggaag ccgggtcggg
     3121 cggaaacata agcccgtgtt tttcgaccgc ctgctgcagc tcgtaagtcg tcagtccggg
     3181 ctggcagacg acgtataaat catcgacgct tatctcgatg attttcttca tccggtcggt
     3241 gccgacgacg atgccgccgt caaccggaac cgcgccgccc gtgtatccga cgccgccgcc
     3301 gcgcgccgtg acgggaaaaa gatattcatt ggcgagcttg agaatttcga ccatttgccg
     3361 cgtcgtttcg ggaaagacga ccgcctcggg cggaaatttt tccttgacgg cgtccgcgcc
     3421 gtaaggctcg accttttccg gttcgactaa aacattttcc gcgccgacaa ttttttgcaa
     3481 tttttcgacg acttcaggtt ttgaagcgtt cgttgtccgg cgcggtttgg cgttgaaatg
     3541 gattgattga aacattttgt aattacttta gtaaatttcg atttctgaac tctttaacta
     3601 atgaatagca gtttgcaaac aaaatagtaa aaggcgtgag aattaggaat aattcaatca
     3661 tcatcgaaac acctaataat tctaatattt taccagggaa ataatctgaa attatttgat
     3721 ttaatgttct ttcgctttca cagattatga ataccgtttg cattaaaagt gccgtgattg
     3781 aagacgttat aagtatcaaa agccatctga tt
//
LOCUS       DASWNE010000765         2002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_273773, whole genome shotgun sequence.
ACCESSION   DASWNE010000765 DASWNE010000000
VERSION     DASWNE010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273773"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..>2002
                     /locus_tag="VGB68_14905"
     CDS             197..>2002
                     /locus_tag="VGB68_14905"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0050579.1"
                     /translation="MKHQTSQFLKFFFSFVLVISAPVFGSAQAKTVSASDSSLAPAND
                     NFANAEQIPMPNPAFVTTQGDVYQAGSEPGEPQHAEKPGGASLWYKITPTETCVMRFL
                     TSNSNFNTLLAVYTGASVDNLTVVGFNDNRYALTNDSQLEVMLTGGQTYYIAVDSFGG
                     IQNPAPHTFSLTAAKFNAPGNDNFAAPYLIAQDTEGNYSGTNYNATKEANEPLIYTSN
                     PNGKSVWFKWTPSISYSATFEITENFNSQIGVYTSASANPTLSQLSRIANNADLNGYA
                     PNRHQATVFAEAGKTYWILVDWHTGGQDIETGNFQLKLARNKFRYSSRLYDHTTKTAV
                     SVFRPSEGVWHDMRDIIQTGLSRTVKFGQNGDVPVAADYDGDGVADHAVARNENGKKM
                     WYILRSDNLGFEAIQWGLSGDRALVGDFDGDGRGDLVAVRSTLQGYVWYIRQSTNGAL
                     RAHQFGALGDRLALGDYDGDRMTDLTAIRNAGGKLVWYISTNPTNAPNNVQAINFGLS
                     SDAAVVEDYYNDGKTDVAVYRAGEMSYWYILRSSDGQFQEIQFGASGDKPQPGDYNGD
                     GKTDVAVFRPSTGSWYIAKPTGVPAQNFYSIQWGAA"
BASE COUNT          481 a          574 c          523 g          424 t
ORIGIN      
        1 aatttcaacc gcaaaaaacc cgactaccga ttcaggtggt ttttcttccg ccggaaaaat
       61 cgcgttttct ttaaatcatt gtaaacacag gaattttctc tattgaaata tgtggcgaag
      121 ctatgtttgg tttccgttat ctttaactta acaacgccgg caacaaaaaa ggcggcggaa
      181 aataaggaga ttaataatga aacatcaaac atcgcaattc ttaaaatttt tcttttcgtt
      241 cgtgttagtt atttccgccc ccgtcttcgg ctcggcgcag gcaaaaaccg tttcggcgag
      301 cgattcgtcg cttgcgcccg ccaacgacaa tttcgccaac gcggaacaga ttccgatgcc
      361 taaccccgct ttcgtcacga cgcagggcga cgtttaccag gcgggcagcg agccgggcga
      421 accgcagcac gcggaaaaac cgggcggcgc gagcctctgg tataaaatca ctccgacgga
      481 aacctgcgtg atgcgtttcc tgacttcgaa cagcaacttt aacactttgc ttgccgtcta
      541 caccggcgca agcgtcgata atttaaccgt cgttggattc aacgacaatc gctacgcttt
      601 gaccaatgac agccagctcg aagtgatgct caccggcggg cagacttatt acatcgccgt
      661 cgattctttc ggcggcattc aaaatcccgc accgcacact ttttcgctga ccgccgcgaa
      721 attcaatgcg cccggcaacg acaatttcgc cgcgccgtat ttaatcgcgc aggatacgga
      781 aggaaattat tcgggcacga attacaacgc gacgaaagag gcaaacgagc ctttgattta
      841 cacttcgaac ccgaacggaa aatccgtctg gttcaaatgg acgccttcca tcagctacag
      901 cgcgactttc gagataaccg aaaattttaa ttcgcaaatc ggcgtttata catcggcgag
      961 cgccaatccg acgctttcgc agctttcccg aatcgccaac aacgccgacc tgaacggcta
     1021 cgccccgaac aggcatcagg cgaccgtttt cgccgaagcc ggaaagactt actggatttt
     1081 ggtcgactgg cacacgggcg gtcaggacat cgaaaccggc aattttcagt tgaagctggc
     1141 gcgcaacaaa ttccgctatt cctcgcggct ctacgaccac acgaccaaaa ccgccgtctc
     1201 cgttttccgc ccgtccgaag gcgtctggca cgatatgcgc gatattatcc agacgggact
     1261 cagccgaacc gtcaaattcg gtcaaaacgg cgacgtgccg gtcgcggcgg attacgacgg
     1321 cgacggcgtc gccgaccacg ccgtcgcgcg caacgagaac ggcaaaaaaa tgtggtatat
     1381 cctgcggtct gacaatctcg gcttcgaggc gattcaatgg ggtttatccg gcgaccgcgc
     1441 tctggtcgga gattttgacg gcgacggtcg gggcgatctg gtcgccgtcc gctcgacgct
     1501 tcaaggctat gtctggtata tccgtcagag cacgaacggc gcgctgcgcg ctcatcaatt
     1561 cggcgctctc ggcgaccggc tcgcgctcgg cgactacgac ggcgatcgga tgaccgattt
     1621 aaccgcgatt cgcaacgcgg gcggcaagct ggtctggtat atttcaacca atccgaccaa
     1681 cgctccgaat aacgtgcagg cgattaattt cggtttaagt tccgacgcgg cggtcgtcga
     1741 agactactac aacgacggca aaacggacgt cgcggtttat cgcgccggcg agatgagcta
     1801 ctggtatatt ctgcgcagca gcgatggtca gtttcaggaa attcaattcg gcgcgagcgg
     1861 cgacaaaccg cagccgggcg attacaacgg cgacggcaaa acggatgtcg cggtgtttcg
     1921 cccgtcgacc ggaagctggt atattgccaa gccgaccggc gttccggcac agaatttcta
     1981 ctcgattcaa tggggcgcgg ca
//
LOCUS       DASWNE010000766         1973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_518326, whole genome shotgun sequence.
ACCESSION   DASWNE010000766 DASWNE010000000
VERSION     DASWNE010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518326"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..808
                     /locus_tag="VGB68_14910"
     CDS             41..808
                     /locus_tag="VGB68_14910"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEY0050580.1"
                     /translation="MKDFSTPLNTENFKADAPENLKILLWDIDGTLMRSTRQGGYVEY
                     FSRTMRQIFGSAGKLDQIQASGMTDTQIMYEALREEGFTPEQIFAAKDDLLKIFKSEM
                     TDVLSKNGEPYEVLGGARRILAETAKNPRFVNALLTGNLSVAAEIKLKSVGLWHYFEN
                     APNAFSEISHDRRELATEAGKLFNDFYKFDFRSEQFIVIGDTPNDIACARALGAKAVA
                     VATGKNHPPQELAAFEPDVLLEDLQDTKKVLEILKNL"
     gene            complement(803..1627)
                     /locus_tag="VGB68_14915"
     CDS             complement(803..1627)
                     /locus_tag="VGB68_14915"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HEY0050581.1"
                     /translation="MRVESRERPDSPWRNRLHEIVFEADTRAGRAFDLTLIWLIVLSL
                     VIIMLESVKSIRENYGEALFVVEWIFTILFSFEYLLRLLSVKKPFRYVLSFYGLVDLL
                     AIAPTYISLLVPGTHYLLMIRVLRLLRIFRILKLSEYLMEANVITSALKASRRKISVF
                     LFAVLAMVTVIGSLMYVIEGEENGFTDIPTSIYWAIVTLTTVGYGDLSPKTALGKILA
                     SLVMIGGYAIIAVPTGIVTAELTRTTKNVSTQICPECHAGAHDADAVFCKYCAAKL"
     gene            complement(1682..>1973)
                     /locus_tag="VGB68_14920"
     CDS             complement(1682..>1973)
                     /locus_tag="VGB68_14920"
                     /inference="COORDINATES: protein motif:HMM:NF012991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050582.1"
                     /translation="LDLASVALIKELTHLPVIVDPSHATGKRSLISATAKAALAIGAD
                     GVIVEVHPQPEIALSDKEQQLTFQGFAEMMRNLQSPVRRILQTAELELAICT"
BASE COUNT          533 a          531 c          493 g          416 t
ORIGIN      
        1 gctgaaagag ccggttaatt aaaaagataa gttttattcg atgaaagatt tttcaacgcc
       61 tttgaacacg gaaaatttta aagcggacgc gccggaaaac ctgaaaattc tcctctggga
      121 cattgacgga actttaatgc gctcgaccag acagggcgga tacgtggaat atttttcgcg
      181 gacgatgcgg caaattttcg gcagcgccgg caagctcgac caaatacaag cgtcgggaat
      241 gaccgacacg cagattatgt atgaagccct gcgcgaagaa ggctttacgc ccgaacaaat
      301 tttcgcggcg aaagacgatt tgctgaagat ttttaaaagc gaaatgacgg acgtgctcag
      361 caaaaacggc gagccgtacg aagttttggg cggtgcgcgc cggattctgg cggaaaccgc
      421 taaaaatccg cgattcgtca acgctctttt gaccggcaat ctttcggtcg cggcggaaat
      481 caaactgaaa tccgtcggac tctggcatta tttcgaaaac gcgccgaacg ccttcagcga
      541 aatatcgcac gaccggcgcg agctggcgac ggaagccgga aagcttttta atgatttcta
      601 caaattcgat ttccgctccg aacaatttat cgtcatcggc gacacgccca acgacatcgc
      661 ctgcgcccgc gctttaggcg ccaaagccgt cgccgtggca accggcaaaa atcacccgcc
      721 gcaggaacta gccgcatttg agcccgacgt tttgctggaa gatttgcagg acacgaaaaa
      781 ggttctggag attctgaaaa atctataatt tagccgcgca gtatttacaa aaaaccgcgt
      841 ccgcgtcgtg cgcgccggcg tgacattcgg gacaaatctg cgtcgaaacg tttttcgtcg
      901 tacgcgtaag ctccgccgtg acgattccgg tcggaacggc gataatcgcg taaccgccta
      961 tcatcacgag cgatgccaga attttgccga gcgcggtttt cggcgacaaa tcgccgtagc
     1021 cgaccgtcgt caatgtcaca atcgcccagt aaatgctggt cggaatgtct gtaaaaccgt
     1081 tttcctcgcc ttcgatgaca tacatcagcg agccgataac cgtcaccatc gccagcaccg
     1141 cgaaaagaaa aacgctgatt ttgcggcggc tggctttcag cgccgaagtg ataacgttcg
     1201 cttccatcag atattccgaa agctttaaga tgcgaaaaat ccgcagcagc cgcaaaacgc
     1261 gaatcatcag caggtaatgc gtgccgggca cgagcaggct gatataagtc ggggcaatcg
     1321 ccagcaaatc gaccaagccg taaaagctca aaacataacg aaatggcttt ttgacgctca
     1381 gaagccgcaa aagatattca aacgaaaata aaatcgtgaa aatccattcg accacgaaaa
     1441 gcgcctcgcc gtaattttcg cggatgcttt tgacgctctc cagcataatt atgacgaggc
     1501 tcaaaacgat gagccaaatc agagtcaaat cgaaggcgcg accggcgcgc gtgtccgcct
     1561 cgaaaacaat ttcgtgcagg cggtttcgcc agggtgaatc gggacgctct cgactttcga
     1621 ctcgcattga aaattattta atcagaccca aagccaaaac gccagattcg gaagcggaat
     1681 cttacgtgca aatcgctaat tccaattccg ccgtttgcaa aatccgccgc acggggcttt
     1741 gcagatttcg catcatttcc gcgaatccct gaaacgtcaa ttgctgttcc ttatccgaca
     1801 gcgcaatctc cggctgcggg tgaacctcga caatcacgcc gtcagcgccg attgccagcg
     1861 ccgctttcgc cgtcgcgctg attaagcttc gctttccggt ggcgtgcgac gggtcgacga
     1921 taacgggcag atgcgtcaat tccttaatca atgcgaccga agccaaatcc agc
//
LOCUS       DASWNE010000767         1584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_202315, whole genome shotgun sequence.
ACCESSION   DASWNE010000767 DASWNE010000000
VERSION     DASWNE010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202315"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VGB68_14925"
     CDS             <1..811
                     /locus_tag="VGB68_14925"
                     /inference="COORDINATES: protein motif:HMM:NF013198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050583.1"
                     /translation="FARGDAHDAETAHGDAQAAARHAHDEEHNTAHDDHDDEEHHHHV
                     DENFKPHESPWMMWVPLAVLAVLSTFGGLVGVPYALSGGAIPNAFEHTLEPIIAKVGH
                     KEAGHGAAEKKDVKHFYASSSESTTGKQGGEANEHPHSPEEIAAERMLALVSVLLAVG
                     GIVIGWLMFRNSPLRQMPKLFERKWYVDEFYNGYIVDPLTRLSREGLWKGFDLTFIDG
                     IVNGAGHFVAAFSGVVRQLQVGFVRSYAAFILLGALVVIGYFIYYGFKLIS"
     gene            890..>1584
                     /locus_tag="VGB68_14930"
     CDS             890..>1584
                     /locus_tag="VGB68_14930"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY0050584.1"
                     /translation="MDFIAENLLTILILLPTLGAVLALAHNMFWKQESHLKWLTLGFT
                     LINFLVSLVLLADRGAVSTSGFFFEKNVPWIRAINTNYHVGVDGLSLWLVILTTFIMP
                     IAVISTWRAVEKRAAAFYIFLLLLESAMIGVFVSLDLLVFYLFFEASLIPMFFLIGIW
                     GGENRVYSAIKFFVYTMLGSLLMLVAIIGLYYIHAEQTGVGTFDFVTLANSLRSGQMA
                     FAAPAGSILFWAFA"
BASE COUNT          317 a          426 c          444 g          397 t
ORIGIN      
        1 ttttgcccgc ggcgacgcgc acgacgccga aacggcgcac ggcgacgctc aagcggcggc
       61 gcgccacgcg cacgacgagg agcacaatac ggcgcacgac gaccacgacg acgaagaaca
      121 tcatcaccac gtcgacgaga atttcaagcc gcacgaatcg ccgtggatga tgtgggttcc
      181 gctggcggtg ctcgccgtcc tttcgacttt cggcggtttg gtcggcgttc cgtatgccct
      241 gagcggcggc gcgattccga atgctttcga gcacacgctc gaaccgatta tcgccaaagt
      301 cgggcacaag gaagccggac acggcgcggc ggagaaaaaa gatgtcaaac atttctacgc
      361 ttcctcgtcc gaatcgacga ccgggaaaca gggcggcgaa gccaacgagc atccgcattc
      421 gccggaagaa atcgcggcgg aaagaatgct ggcgctggtt tccgttcttc tggcggtcgg
      481 cggaatcgtt atcggctggc tgatgttcag aaacagcccg ctgcggcaaa tgccgaaatt
      541 gttcgaacgc aaatggtatg tcgatgaatt ttacaacggc tacatcgtcg acccgctgac
      601 cagactttcg cgcgagggct tgtggaaagg cttcgattta acctttatcg acggcatcgt
      661 caacggcgcg gggcatttcg tcgcggcatt cagcggagtc gtccgccagc ttcaggtcgg
      721 attcgtccgc agctacgccg cgtttatcct gctcggcgcg ctggtcgtca tcggttattt
      781 tatttattac gggtttaagc taattagtta gcagtcagca gtcagcagtc agccgtcagc
      841 taaaactgac cgttgactgc tgactgctga ctgccgacca ctaaaaaaaa tggatttcat
      901 agcagaaaat cttttaacaa ttcttatttt gcttccgacg ctcggcgctg ttctggcgct
      961 cgcgcacaat atgttctgga agcaggaaag tcacctgaaa tggctgacgc tcggctttac
     1021 gctaatcaat tttctcgtct cgctcgtttt gctcgccgac cgcggcgcgg tttcgacgag
     1081 cggatttttc tttgagaaaa acgttccgtg gattcgggcg ataaacacga attaccacgt
     1141 cggcgtcgac ggtctcagct tatggctggt aattttgacg acgtttatta tgccgattgc
     1201 cgtcatctcg acgtggcggg cggtcgaaaa acgggcggcg gctttttata tctttctgct
     1261 gctgctggaa agcgcgatga tcggcgtttt cgtatctttg gatttgctgg ttttctatct
     1321 gttcttcgag gcttcgttga ttccgatgtt tttcctgatc ggcatctggg gcggcgaaaa
     1381 ccgcgtctat tcggcaatca aattttttgt ttacacgatg ctcggctcgc ttttgatgct
     1441 cgtcgccatc atcgggcttt attatattca cgccgagcag accggcgtcg gaacgttcga
     1501 tttcgtcacg ctcgccaatt cgctgcgaag cgggcagatg gcttttgccg ctccggcagg
     1561 ctcgattctt ttctgggctt ttgc
//
LOCUS       DASWNE010000768         4870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_337495, whole genome shotgun sequence.
ACCESSION   DASWNE010000768 DASWNE010000000
VERSION     DASWNE010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337495"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VGB68_14935"
     CDS             complement(<1..769)
                     /locus_tag="VGB68_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050585.1"
                     /translation="MKKELFIQAAFIFLILICGTTAFAAPANDNYQNAVVTTLPVPLM
                     ELQGSNFGATKEPGEPAHANNIGGQSVWYRIVVPETTVVKIWIENTSAANFMNTLLAV
                     YKGSTPSDFNLVAHNDNMRDTLLSKVQVTLKPGNIYHVAVDGFNDNGNIAAGNFRIRF
                     EKLGAPANDLIDAWNPDARLPMSTHGFTSGTNRNATKEPGEPNITNNVGGKSVWYFWR
                     APDTRSMTVKLTCNPFVQECFDMQLAVFKGMNDKVASN"
     gene            831..977
                     /locus_tag="VGB68_14940"
     CDS             831..977
                     /locus_tag="VGB68_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050586.1"
                     /translation="MKKTGRHTTFLSSKKRLVFQSIGLKIAVFPKIFPPPLGIYVAAA
                     ETFI"
     gene            1105..1911
                     /locus_tag="VGB68_14945"
     CDS             1105..1911
                     /locus_tag="VGB68_14945"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0050587.1"
                     /translation="MAENILLRNNVKISGRGKQPLMFAHGFGCDQNMWRFITPAFEED
                     YKIVLFDYVGSGKSDLKAYQPARYGDLSGYARDILEICDALALEDVIFAGHSVSSMIG
                     VLAAIERPELFKSLVLVVPSPCYINHPPDYVGGFERADIEGLLDMMDKNYIGWASFLA
                     PVIMKNPEHPELSRELEESFCSTDPRIARRFAEATFYSDNREDLSKLKTPSLVIQVTD
                     DAIAPVSVGEFMHRNLPESTLHLMDATGHCPHMSHPHETIKIIKDYLNKN"
     gene            1926..3467
                     /locus_tag="VGB68_14950"
     CDS             1926..3467
                     /locus_tag="VGB68_14950"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0050588.1"
                     /translation="MDELLDNAPCGFLAFADDGKIVVANTTLLRLLGYGRDEILELHI
                     EKIFSIAGRIFYQTHFFPLLKLKNQVEEVYLSLRSKSGADIPVLVNAARRERGGRFFN
                     DCVFMPMRQRNQYEDEILKAKKEAEEATRAKDEFLSVVSHELRTPLNGILGWAQILQT
                     RNPDSETVKRAVDAIVRGARAQSKLIEDILDFTRIISGKLRLDVRQVDLVLIVESAMD
                     VITPAANAKNISLQTVLDSNSFVSGDIERLQQILWNLLSNAVKFTPKGGRVQIRVQRV
                     NSSVEIVVSDTGKGISPEFLPFVFERFKQSDNSTSRRYGGLGLGMAITRHIVELHGGT
                     IRAESPGEDLGTTFTVSLPVSVSSSARPVPAEETGEISPADENGSRADSLPPLDGLCV
                     LIVDDERDARDLLIAVLSRQGANVVVAETVADAVKKFQAQAPDLIISDIEMPDEDGFS
                     LIKKLRAFNRTQTRKIPAVALTAHARPSERLKILSAGFQMHLAKPVDTSELIAVIANF
                     ADLNK"
     gene            complement(3527..4138)
                     /locus_tag="VGB68_14955"
     CDS             complement(3527..4138)
                     /locus_tag="VGB68_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050589.1"
                     /translation="MFCPGCGLEERQSNQFCRACGTDLRQIRFALERPDNITASAVSA
                     RDEIGRAVAAKIRETQSARELKKVAEDVLPAIEKFLESPEEKRLRRVRSGVIVASIGI
                     GAAISFFYASLLMADTGILLIAGAGAITFFIGLSLIINALFFTVPRKSLPDNSIDAES
                     QRALDRTDAETNELLLPESNNIFSSVTEHTTQHLREKQRSVKR"
     gene            complement(4314..4760)
                     /locus_tag="VGB68_14960"
     CDS             complement(4314..4760)
                     /locus_tag="VGB68_14960"
                     /inference="COORDINATES: protein motif:HMM:NF024287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3828 domain-containing protein"
                     /protein_id="HEY0050590.1"
                     /translation="METNAANISGAAQQTAPGALVKDLYKTHDKDNGAILDGKSRRLL
                     DKYFDKTLADFIWKDLTTHKDEVGVLDFDPFYNAQEVQIKNFKIGAPKVVGEKAAVTV
                     TFQNFDRREALNYALVRQGGAWKISDIKYTDGTSLLGYFKEDAKRK"
BASE COUNT         1121 a         1267 c         1269 g         1213 t
ORIGIN      
        1 cattcgaagc gactttatcg ttcataccct taaaaacggc gagctgcata tcaaaacatt
       61 cctgcacgaa cggattgcag gtcagtttca ccgtcatcga acgagtgtcc ggcgcgcgcc
      121 agaaatacca gaccgatttg ccgccgacat tatttgtaat gttcggctcg cccggctctt
      181 tggtcgcgtt gcggttggtt ccgctggtaa acccgtgcgt ggacatcggc agccgggcgt
      241 ccggattcca ggcatcgatc aggtcattag ccggcgcgcc cagcttttca aaccggattc
      301 tgaaattgcc ggcggcgata ttgccgttat cgttaaatcc gtcaaccgcg acgtgataga
      361 tgtttcccgg tttaagggtc acctgaacct tgctaagcaa agtatcgcgc atattgtcgt
      421 tatgcgcgac gagattgaaa tcgctcggag tagaaccttt gtaaacggca agcagcgtat
      481 tcataaagtt tgcggcagaa gtattttcta tccaaatctt gacgacggtc gtctccggca
      541 cgacgattct gtaccagacg gattgcccgc cgatattatt ggcgtgagcg ggttcgcccg
      601 gctctttcgt cgccccgaaa ttcgagccct gaagctccat caaaggcacg ggcagggtcg
      661 tcaccacggc attttgatag ttgtcgttgg cgggagcggc aaacgcagtc gtgccgcaaa
      721 ttaatatcaa aaaaataaat gccgcttgaa taaatagttc ctttttcata attgctcctt
      781 ataattttcc gcccgtcgaa tctttaattc ttcaggatgt ttctaagata atgaaaaaga
      841 cggggcggca caccacgttt ttaagtagta aaaagcggct ggtttttcag tcaatcggct
      901 taaaaatcgc ggttttcccc aaaatatttc cgccgccgct gggtatttac gtagcggcgg
      961 cggaaacctt tatttaatcg gtatctgaag gcgatgaatc aatcgccggg aaaagaagcg
     1021 attaatttaa aatttgcttt cgcgcgaaga ttactttacc gtttatagat acggttcaat
     1081 acacggattt tctctggatt taagatggct gaaaatattc tcctgcgcaa caacgtcaaa
     1141 atttcggggc gaggcaagca gcctttgatg ttcgctcacg gcttcggctg cgaccagaat
     1201 atgtggcgct ttatcacgcc cgcttttgaa gaagattaca aaatcgtttt gtttgattac
     1261 gtcggttccg gcaaatccga cctcaaagct tatcagcccg cgcgctacgg cgatttgagc
     1321 ggttacgcgc gcgacattct ggaaatctgc gacgcgctcg cgctggaaga cgtgattttt
     1381 gccggtcatt cggtcagcag tatgattggc gtgctggcgg ctatcgaaag accggagctt
     1441 tttaaaagcc tggttctggt cgtgccttcg ccgtgttata tcaatcatcc gccggattac
     1501 gtcggcggtt tcgagcgcgc cgacatcgaa ggcttgctcg atatgatgga caaaaattac
     1561 atcggctggg cgagctttct cgcgccggtg attatgaaaa atcccgagca ccccgagctg
     1621 agccgggagc tcgaagaaag cttctgctcg accgacccgc gcattgcccg ccggtttgcc
     1681 gaagccacgt tttattcgga taatcgcgag gatttgtcga aattaaaaac gccttcgctg
     1741 gttatacagg tcaccgacga cgcgattgcg cccgtttcgg tcggcgaatt tatgcaccga
     1801 aatttaccgg aaagcactct gcatttgatg gacgcgaccg ggcactgccc gcatatgagc
     1861 catccacacg agacaatcaa gattatcaaa gattatctga ataagaatta acccgttccg
     1921 cgccgatgga tgaattatta gacaatgcgc cgtgcggatt tctcgccttt gccgacgacg
     1981 gtaaaatcgt cgtggcgaat actactctgc tccggctgct cggatacggg cgcgacgaga
     2041 ttttagagct tcacatagaa aaaatcttct ccatcgccgg gcgcatcttt tatcaaacgc
     2101 atttttttcc gctgctcaaa ttaaaaaatc aggtcgaaga agtttatctc tccctgcgct
     2161 cgaaatcggg cgcggacatt cccgttttgg ttaatgccgc gcggcgcgag cgcggcgggc
     2221 gtttttttaa cgactgcgtt tttatgccga tgcgccagcg caaccagtat gaagacgaga
     2281 ttttaaaggc gaaaaaggaa gccgaagagg cgacgcgcgc caaggacgaa tttttgtcgg
     2341 tcgtctcgca cgagctgcgc acgccgctca acggaattct cggctgggcg cagattcttc
     2401 agacccgaaa tcccgattcg gagacggtca agcgcgccgt cgacgccatc gtccgcggcg
     2461 cgcgggcgca atccaagctt atcgaagata ttctggattt tacgcgcatt atttccggca
     2521 agctgcggct cgatgtccgg caggtcgatt tggtattgat tgtcgaatcg gctatggacg
     2581 tgataactcc ggcggcaaac gccaaaaaca tcagcctgca gacggttctg gattcgaaca
     2641 gttttgtttc cggcgacatc gagcgattgc agcagatttt atggaatctg ctctcgaacg
     2701 ccgttaaatt cacgccgaaa ggcgggcgcg tgcagattcg cgtgcagcgc gtcaattcga
     2761 gcgtcgaaat cgtcgtcagc gatacgggca aaggcatcag cccggaattt ctcccttttg
     2821 tcttcgagcg atttaagcag tcggacaatt cgacgagccg gcgctacggc ggcttgggtt
     2881 tgggaatggc gattacgcgt catattgtcg agcttcacgg cggcactatt cgggcggaaa
     2941 gccccggcga agacctcggc acgaccttta cggtcagttt gcccgtttcc gtttcaagct
     3001 ccgccagacc ggttcccgcc gaagaaaccg gagaaatctc tccggctgac gaaaacggct
     3061 cacgggcaga ttcgttgccg ccgctggacg ggctgtgcgt tttgattgtc gatgacgaac
     3121 gcgatgcgcg cgatttgctg attgccgttt tatcgaggca aggcgccaac gtcgttgtcg
     3181 ccgaaaccgt cgccgacgcg gttaaaaaat ttcaagcgca agcgcccgac ctgattatca
     3241 gcgacatcga aatgccggac gaagacggtt tttccctgat aaaaaaactc cgcgcgttta
     3301 accggacgca gacgcggaaa attcccgccg tcgcgctgac ggcgcacgcg cgcccgtccg
     3361 aaaggctgaa aattttgtcg gcgggctttc aaatgcatct tgccaaaccg gtcgatactt
     3421 cggagctgat tgcggtcatt gccaactttg cggatttgaa taaatagagc aggcaggcga
     3481 aaaacggtaa acggcaagcc gccggtcgca gttgaaaagc atcgagctac cgtttaacgc
     3541 ttctttgttt ttcccttaaa tgctgcgtcg tgtgttcggt cacggaagaa aagatattgt
     3601 tcgattcggg caataaaagc tcgttggtct cggcgtcggt acggtcgagc gcgcgctggc
     3661 tttcggcgtc aatcgaatta tcgggcaggc tctttcgcgg aacggtaaaa aacaaggcgt
     3721 ttatgataag gctcagaccg ataaaaaaag ttatcgcgcc cgccccggca atcaaaagga
     3781 tgcctgtgtc tgccatcaaa agactggcgt aaaaaaagct tatcgccgcg ccgatgccta
     3841 tcgaagcgac aatcacgccg ctgcgcacgc ggcgcagacg cttttcttcg ggcgattcca
     3901 gaaatttttc gattgccggc aaaacgtctt cggcaacctt tttcaattct cttgccgatt
     3961 gcgtttcgcg aattttcgcc gccaccgcgc gcccgatttc gtcgcgcgcc gagacagccg
     4021 aagccgtgat attgtcggga cgctccagcg cgaaacgaat ctgccgcaaa tccgtcccgc
     4081 acgcgcggca aaactgatta gactggcgct cttccaaacc gcaacccgga caaaacatat
     4141 ttttcggaat ctccctgaat ctggatttct gtatcgaccg aaaaagtata cgctcaaaat
     4201 ttcgctttag ttcgacggcg acaagcaaac gaattttaac gtgcaaaatt tattcggcgt
     4261 taaaattcta cgaaaataag attgtgtctt taccgggaaa acgcctctgc cgcctatttt
     4321 cttttcgcgt cttctttaaa atatccaagt aaactcgtgc cgtccgtgta tttgatgtcc
     4381 gagattttcc acgcgccgcc ttggcgcacg agagcgtaat tcaaagcttc acggcggtcg
     4441 aaattttgaa acgtgacggt aacggcggct ttttcgccga cgactttcgg agcgccgatt
     4501 ttaaaatttt taatctgcac ctcctgcgcg ttgtaaaacg ggtcgaaatc cagcacgccg
     4561 acttcgtcct tgtgcgtcgt caaatctttc cagataaaat cggcgagcgt tttatcgaaa
     4621 tatttgtcga gcagtcggcg gcttttgccg tcgagaatcg cgccgttgtc cttgtcgtgc
     4681 gttttgtata aatctttaac gagcgcgccg ggcgcggttt gctgcgccgc gccgcttatg
     4741 ttcgcggcgt ttgtttccat cgtttcggcg ctcgcgtttg attcgattgt cgttgcggtt
     4801 gccgcgttat ttgccgtttt cgttctgttt gcggaaacgc agctaaaagc cgaaattaaa
     4861 atcgtcaccg
//
LOCUS       DASWNE010000769         5141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_61489, whole genome shotgun sequence.
ACCESSION   DASWNE010000769 DASWNE010000000
VERSION     DASWNE010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5141
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61489"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VGB68_14965"
     CDS             <1..425
                     /locus_tag="VGB68_14965"
                     /inference="COORDINATES: protein motif:HMM:NF028473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="delta-60 repeat domain-containing protein"
                     /protein_id="HEY0050591.1"
                     /translation="PNGEIMVLFSYKIMILNADGSFNRDVLSGPVTINRKLFSPFRFD
                     VQPDGKIVSLGSVRWGSTDQIAVARFNPDGEVDTSFAARGLYLRQTNQPAALDRILIQ
                     PDGKILGVSGVFTVTDIALMRLLGSSSPAPSTGKPLDR"
     gene            complement(580..1488)
                     /gene="uvsE"
                     /locus_tag="VGB68_14970"
     CDS             complement(580..1488)
                     /gene="uvsE"
                     /locus_tag="VGB68_14970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009650 - UV protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UV DNA damage repair endonuclease UvsE"
                     /protein_id="HEY0050592.1"
                     /translation="MEAKMQCVKSNLGLVCITNSDAVRYKTVTRKRLLSFDEATQKEM
                     LRALYRENINRLNRGIEFCTTNGINLYRLTSGLFPFSDEPVGAEILDEFTARLAETGK
                     TAIAKNLRLVIHPDQFVVLSSDSENVIKNSVKILKMHARTLDLLRQPRSEWTTINIHG
                     GKGDRIDRLVQSIENLPDEIRSRIAFENDEHAYSSAQILEVCRRAGVRMVFDAHHHVC
                     REKLDDYNHPSVAESFWAARETWKNPEWQLVHISNGREKFQDRAHSDLIFTMPEVFRF
                     APWIEVEAKHKELAIAKLQANWLNAE"
     gene            complement(1553..3985)
                     /locus_tag="VGB68_14975"
     CDS             complement(1553..3985)
                     /locus_tag="VGB68_14975"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HEY0050593.1"
                     /translation="MFCHLFRSIFLCLFVGCALILTPVAAQKKDKKTKKEEIKREIPT
                     PKSVLGFHPTDDKTIADWSQITNYFGKLDAASDRLKVETLGETTLKKPFIVAFISAEE
                     NIRNLDKYREINARLADPRKIKDNAELESLIAQGKTVVSISCSIHSTEIVASQMSMNL
                     AYGLATAQDAETKEILQNTILMLIPSANPDGIDIVAEWYRKTLGTLYEGSNPPELYHH
                     YAGHDNNRDWFMMNLAETRLVTKLFWQQWFPQIVYDVHQMGQSGARFVIPPFFDPPNP
                     NIPPLVLREVGLIGYKMAADLQAKNVAGVATNTTFDTWWHGGFRSAPYYHNSIGILSE
                     AASANLMSPVRVSEEKMRERRERGLSSPFEYSTAQPNPWRPTVWGPREIAEIEMIASR
                     SLLQMAAKFRANYLRNFAELNRSSAQTGANPAQPLAYVVFAGQAREEIVARFLEILMW
                     QGIEVYKMNSELRTLMPAMGEAPMEVPLGSFLVFLDQPQRNNVEALFGRQVYPNRVDE
                     KGNVDQPYDVAGWTLPLQMDIEYHAVTQIVEHPTERRLEKMIDINQVRQTLNLEAARA
                     SFVKLSNPLKSTPKIGIYKGLATSSDEGWTRWTFDTFQIPFQSVKDADVKNGNLKARF
                     DVIILPSQREEQILKGLKEENYPKELTGGIGAAGAANLQKFVEDGGRLICFDDSCELV
                     IKQFNLPLKNVLSGLKRSEFYCPGSILQIDVDTVNPLAEGLKKQMPAYFIDSSAFEIS
                     DANKVKSVASYAQKNALLSGWLLGEKYLNGKTALAETAHGKGKIVLFGFRPQHRGQSF
                     ATFPFVFNALEN"
     gene            3974..4786
                     /locus_tag="VGB68_14980"
     CDS             3974..4786
                     /locus_tag="VGB68_14980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY0050594.1"
                     /translation="MTEHLSNKPFAELIEQNQKIVFPVEKFSTESRAAGKLRYYYGWF
                     VAALLLLFIATPALVFLWLINRRKWLYPLASWGARTWLKACGARVAVRGLENIKRDEN
                     YVFISNHRSYLDTAMLFAYTGHRIGLVAKRELLKVPVLGQGMNFVNIIAMDRSDPERA
                     RRSMQKAKEVLESGYSFGVFAEGTRAMRGELLPFKKGAFHLALQTEATIVPVAIKNTD
                     SMMGKKTGVAYRGTIEMVLLPPIETKNLSAEKDLMNLLKRTRAVIAEELDNR"
BASE COUNT         1165 a         1337 c         1326 g         1313 t
ORIGIN      
        1 tgccgaacgg cgagataatg gttcttttct cctataaaat aatgatctta aatgcggacg
       61 gcagttttaa ccgggacgtt ttaagcggac ccgttacgat aaatcgaaag ctattttctc
      121 ctttcaggtt cgatgtgcag ccggacggca aaatagtctc tctcggctcg gtgcgctggg
      181 gcagtacgga tcagattgcc gtcgccagat tcaacccgga cggcgaggtc gatacatcgt
      241 ttgccgccag aggattatac ctgcggcaaa caaatcaacc ggcggcgctc gatagaatct
      301 taatccagcc ggacgggaaa atcctcggcg tcagcggcgt gtttaccgtg acggacatcg
      361 ccctgatgcg gcttctcggc agcagctcgc ccgcgccgtc gaccggcaag cctttagacc
      421 gctaaagctc tttccggctt tttttcttcg atagttcagc ataaaaagca gcgccggaaa
      481 attttaaccg gcgctgcttt ttattttatt cgcccgtcaa accggaaagc ggcaagcaaa
      541 cgccggaatc gcggcgcggg cggaatatac agccggcttc tattccgcgt ttagccagtt
      601 cgcttgcagc tttgcgatcg ccagttcctt gtgtttcgct tcgacctcaa tccacggcgc
      661 aaagcgaaaa acttccggca tcgtaaaaat caaatcgctg tgcgcgcggt cttgaaattt
      721 ttcgcgcccg ttcgagatgt gaacgagctg ccattccgga tttttccacg tttcacgcgc
      781 cgcccagaag ctttccgcca ccgacggatg attgtaatcg tccagctttt cgcggcagac
      841 gtgatgatga gcgtcgaaaa ccatccgcac gccggcgcgg cggcaaactt ccagaatctg
      901 agccgaactg taggcgtgtt cgtcgttttc aaacgcgatg cgcgagcgga tttcgtcggg
      961 cagattttcg attgattgaa caagcctgtc gatgcggtcg ccttttccgc cgtgaatgtt
     1021 tatcgtcgtc cattccgaac gcggctgccg cagtaaatcc aaagtccgcg cgtgcatctt
     1081 caaaattttg acgctgtttt taataacgtt ttccgaatcc gagctcaaga ccacaaactg
     1141 gtctggatga atgacgagcc gcagattttt ggcgattgcc gtttttccgg tttcggcaag
     1201 ccgagcggta aattcgtcta aaatctccgc tccaaccggc tcgtcggaaa aaggaaaaag
     1261 cccggacgtc agacgataga gatttatccc gttcgtcgtg cagaattcga ttccccggtt
     1321 caggcgattg atgttttccc gataaagcgc gcgcagcatt tccttctgcg tcgcctcgtc
     1381 aaacgacaaa agccgcttgc gcgtgacggt tttgtaacga acggcgtcgg aattggtgat
     1441 gcaaaccagc ccgagattgc ttttgacgca ttgcattttc gcttccataa atctatttaa
     1501 ccgcaaagac ggcgagcgat gcaaagaagt aggaaacggc aaaagcagaa gattaatttt
     1561 ccagagcgtt aaaaacgaac ggaaacgtcg cgaagctttg accgcgatgc tgcgggcgaa
     1621 agccgaacaa aacgattttg cctttgccgt gagcggtttc ggcgagcgcg gttttgccgt
     1681 tcagatattt ttcgcccaaa agccagccgg aaagcagcgc gtttttctgc gcgtagctcg
     1741 ccaccgattt cactttattc gcgtccgaga tttcaaacgc cgagctatcg ataaaatagg
     1801 cgggcatttg ttttttcaag ccttcggcaa gcggatttac agtgtccacg tcaatctgca
     1861 aaatcgagcc gggacaatag aattcgcttc gtttcaagcc ggacaggaca ttcttcagcg
     1921 gcagattaaa ttgtttgata accagctcgc acgagtcgtc aaagcaaatc agcctgccgc
     1981 cgtcttcgac aaacttttgt aaattcgccg cgcccgccgc accgattccg cccgtcagtt
     2041 ctttcggata attttcttct tttaagcctt tgagaatttg ttcttctctc tgcgacggca
     2101 gaattatgac gtcgaaacgc gcctttagat ttccgttttt cacgtccgca tcctttaccg
     2161 attgaaacgg aatttgaaac gtgtcaaacg tccagcgcgt ccagccttcg tccgacgaag
     2221 tcgctaagcc tttgtaaatt ccgattttcg gcgtcgattt caaaggattc gataatttaa
     2281 cgaaagacgc gcgcgccgct tcgagattca gcgtctgcct cacctgattg atgtcaatca
     2341 ttttttccag ccgcctttcg gtcggatgtt cgacgatttg cgtgacggcg tggtattcga
     2401 tgtccatctg aagcggcagc gtccagcccg ccacgtcata cggctggtcg acgttgcctt
     2461 tttcgtcgac ccgattcgga taaacctgtc tgccgaaaag cgcttcgacg ttgttgcgct
     2521 gcggctggtc gagaaaaacc agaaatgagc cgagcggcac ttccatcgga gcttcgccca
     2581 tagcgggcat caaggttctc aattcgctgt tcattttata aacctcgatg ccctgccaca
     2641 tcagaatttc cagaaaacgc gcgactatct cttcgcgcgc ctgcccggcg aaaacgacgt
     2701 aagccagcgg ctgcgccgga ttcgcgccgg tttgcgcgct tgagcgattc aattcggcaa
     2761 aatttcgcag gtaattggcg cggaatttcg ccgccatctg caaaagcgag cgcgaggcaa
     2821 tcatctcgat ttcggcaatc tcacgcggtc cccagacggt cggtcgccac ggattcggct
     2881 gcgccgtcga atattcaaac ggcgaactca gcccgcgctc gcgccgctcg cgcatttttt
     2941 cttcgctcac gcggacgggc gacatcaggt tcgcgcttgc cgcctccgac aaaattccga
     3001 ttgagttgtg ataatacggc gcggagcgaa aaccgccgtg ccaccacgta tcgaacgtcg
     3061 tgttcgtcgc tacgcccgcg acgtttttcg cctgtaaatc cgccgccatt ttgtagccga
     3121 ttagaccgac ttcgcgcaga acgagcggcg gaatattcgg attcggcggg tcgaaaaacg
     3181 gcggaatgac aaagcgcgcg cccgattgtc ccatctggtg cacgtcgtaa acaatctgcg
     3241 gaaaccattg ctgccaaaag agttttgtga ccagccgcgt ttccgcgaga ttcatcataa
     3301 accagtcgcg gttgttgtcg tgtccggcgt aatgatgata aagctccggc ggattagagc
     3361 cttcgtaaag agttccgagc gttttccggt accattcggc gacgatgtcg atgccgtccg
     3421 gatttgccga aggaatcagc atcaaaatcg tgttttgcag aatttccttt gtttccgcgt
     3481 cctgcgccgt cgccagcccg taagccagat tcatagacat ctgcgaggcg acgatttccg
     3541 tcgagtgaat cgagcaggaa atcgagacga cggttttgcc ctgcgcaatc aagctttcga
     3601 gctcggcatt gtctttgatt tttcgcgggt cggcgagcct ggcgttgatt tccctgtatt
     3661 tatcgagatt ccggatgttt tcttccgccg aaatgaaagc gacgatgaaa ggttttttca
     3721 aagtcgtttc gcctaaagtt tcgactttca ggcggtcgct cgccgcgtcg agttttccaa
     3781 aataatttgt aatctgcgac caatcggcga tggttttatc gtcggtcggg tgaaagccga
     3841 gaacggattt cggcgtggga atttcgcgtt ttatttcttc cttttttgtt tttttatctt
     3901 tcttttgcgc ggcgacaggc gttaaaatca aagcgcagcc gacgaacaga cacaaaaaaa
     3961 tcgaacgaaa cagatgacag aacatttatc taataaacct ttcgcggaat tgattgagca
     4021 gaatcaaaaa attgtttttc ctgtcgaaaa gtttagcaca gaatcgcgcg ccgccggcaa
     4081 gctgcgttat tactacggct ggtttgtcgc cgccctgctg cttttattca tcgccacgcc
     4141 ggcgctggtt tttttgtggc ttatcaatcg ccggaaatgg ctttatccgc tggcgagctg
     4201 gggcgcgcga acctggctca aagcgtgcgg cgcgcgcgtc gcggtgcgcg ggctggaaaa
     4261 tataaaacgc gacgaaaatt acgtttttat ctcaaatcat cgctcgtatc tcgacacggc
     4321 gatgctgttt gcctatacgg gacatcgaat cggtctggtg gcgaaacggg agcttttgaa
     4381 agtgcccgtt ctcggtcagg gaatgaattt cgtcaacatc atcgcgatgg accgcagcga
     4441 cccggaacgg gcgcgccgct cgatgcagaa agcgaaggaa gttctggaaa gcggctattc
     4501 tttcggcgtc tttgccgaag ggacgcgggc gatgcgaggc gagcttttgc cgtttaaaaa
     4561 aggcgcgttt catctggcgc tgcaaaccga agcgacgatt gtcccggtgg cgataaaaaa
     4621 cacggattcg atgatgggca aaaaaaccgg cgtcgcttat cggggaacaa tcgagatggt
     4681 tttgctgccg ccgattgaaa cgaaaaattt gtcggcggaa aaagatttga tgaacttact
     4741 gaaaaggacg cgggcagtga ttgcggagga gctggataac cggtagtgga tagctttgtt
     4801 tttcactgtt tcagcacgct agtagaagct atgcaatttc attgcaagaa tttattatgc
     4861 tttcttttct ttgtctgaaa agattaaatc aagcaaattc tatttgcgtt ccgattctta
     4921 aacctctgca ctttcagtgc agagtggttt agtctttcac tttttttact tttctatctt
     4981 ttattcgccg ttcatttcct cgaaatccag ctgctccaga acgcggcggg cttcttcgga
     5041 ttcgtattca aaggcttgct gttcgagcac gttgcggacg atttcaaaat aaaccgggtc
     5101 gtcaatcatc tgcataagcg ccgagaccgg tacgagcgcg a
//
LOCUS       DASWNE010000770         3937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_518491, whole genome shotgun sequence.
ACCESSION   DASWNE010000770 DASWNE010000000
VERSION     DASWNE010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518491"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..731)
                     /locus_tag="VGB68_14985"
     CDS             complement(9..731)
                     /locus_tag="VGB68_14985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050595.1"
                     /translation="MNQIKLLKRAAMAIGFSLPLAIAAHAQSGDIKTPYTGKVSCGQI
                     LKWGTKGTIHYYEKRLNAETKWAYGDYNRCKFDRNSAGLKKLSADKQKIVERLRDALE
                     LYFRSFYTMQAITSGGGEPFELDSLASHSDVEDLIGKAVLIFSKPSAPQPAWRKQAEA
                     LLSKAETRLPELTKTPEAEDFDWLDATKEDDRETIKSSQADYKKEADEMRESLKNFRA
                     LLKDLPDSLALLSSELLDDILP"
     gene            complement(839..2182)
                     /locus_tag="VGB68_14990"
     CDS             complement(839..2182)
                     /locus_tag="VGB68_14990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050596.1"
                     /translation="MQNLEHITEESLIAYGKRELAAAETLAVQKHISACEICREKLAR
                     KFDAPKMFSALREDFAFDDLADEPEHLPYERLALFADGKLDDVEREIAESHFAICAEC
                     SKDLTDLRSFQTIAAAPVEPKKSAETQSFWRKLFAFDSIGSFAPVAAAILLAVLFGAW
                     FLLRGDKNEIAQTNGNKSTPAPTISISNVNPANDSPNVDALPENSASPDVSPAPENLS
                     KNEPLLALDDGQVTVDENGNVKGLENLSPQSQKAIRQSLQTGRVSVSNAVNSLGGVSG
                     GVLMSGGNEENGVPFALASPIGKVIRENQPVLRWKPLKDATAYSVTIVDDKFRVVADS
                     GKLTATSWKPSKPLPRGANYSWQVTAIKVDGSETVSPASPAPQARFRVVEQTTIDEIG
                     RLEKSGRRSHLVLGVLYAQAGLKQEASREFEMLVKQNPKSTLARKLLAGVQGKSK"
     gene            complement(2320..3369)
                     /locus_tag="VGB68_14995"
     CDS             complement(2320..3369)
                     /locus_tag="VGB68_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050597.1"
                     /translation="MNAGQTDSLLRLFAQTSDADEQDRALSALLTEHAAPVVSKIIRY
                     KTRGANEEGEEIYSDVMLQLVGRLQQLRAVGNGSSIDDFNSYVAVTTYNACDRFLSRK
                     YPQRRRLKNGLRYLLAHREGFALWQDEKQNYVGGFRRWQFGASRDDSSLKIQSLREDA
                     RSFERDVAVQNSWSEQKNAYELLTAIFEWTGAPVEIDLLVNVCAAWWNITDETIEVDA
                     ANTNEKGEQRPQMQLADARPDAYTETERRDYLQRLWNEIADLPPRQRASVLLNLKDEN
                     GRGVIDLWLVVGVASVEKIAAVLEMSTEAFAELWNDLPLDDNRIAEKLNLTRQQVINL
                     RKSARDRLARRMKGG"
BASE COUNT          887 a         1059 c          933 g         1058 t
ORIGIN      
        1 cgtaaatttt acggcagaat gtcgtcgagc agctcgcttg aaagcagcgc cagcgaatcg
       61 ggcaaatcct tcagaagcgc gcggaaattt ttcaaacttt cccgcatctc gtccgcctct
      121 tttttataat ctgcctgcga actcttaatc gtttcgcggt cgtcttcctt tgtcgcatcg
      181 agccaatcga aatcctctgc ttccggcgtt ttcgtcaatt caggcaatcg cgtctcggct
      241 ttcgacaata acgcttcggc ttgttttcgc caggcgggct gcggcgcgga cggtttcgag
      301 aaaatcaaaa cggctttccc gattaaatct tccacgtccg aatggctcgc caagctatcg
      361 agctcaaacg gttcgccgcc gccggacgta atcgcctgca tcgtgtaaaa cgagcgaaaa
      421 taaagctcaa gcgcgtcgcg caatctttcg acaatttttt gtttgtcggc ggaaagtttt
      481 ttcaatccgg cggaattccg gtcgaattta cagcgattgt agtcgccata cgcccatttc
      541 gtttcggcgt tcagccgttt ttcgtagtaa tggattgtgc ctttcgttcc ccatttcaaa
      601 atctgcccgc acgaaacttt gcccgtgtaa ggcgttttaa tgtcgccgct ctgcgcgtgc
      661 gcggcgattg ccagcggcaa agaaaagcca atcgccattg ccgccctttt taaaagtttt
      721 atttgattca taaatcctcg ccgataattt gttcgagaaa aattcgttct ttcccgtaaa
      781 caaaacgagg cggaaaaagc gcggatatta ccggagaaat tacggaaatt tttgagtttt
      841 atttcgattt gccttgaacg ccggcgagaa gcttgcgggc gagcgtggat ttcggatttt
      901 gcttgaccag catttcaaac tcgcggctgg cttcctgctt caaacccgct tgcgcgtaaa
      961 gcacgccgag cacgagatgc gagcgcctgc cggatttttc aagcctgccg atttcgtcaa
     1021 tcgtcgtttg ttcgacaacg cgaaaacgcg cttgcggcgc gggcgacgcg ggcgagacgg
     1081 tttcgcttcc atcgacttta atcgccgtca cctgccacga ataatttgcc ccgcgcggca
     1141 gcggtttaga cggtttccag cttgtcgcgg ttaatttccc gctgtcggcg acgacgcgga
     1201 atttatcgtc gacaatcgtg accgaatagg cggtcgcgtc tttcaagggt ttccagcgca
     1261 aaaccggctg gttttccctg ataaccttgc cgattggcga agcaagcgcg aacgggacgc
     1321 cgttttcctc gtttccgccg ctcatcaaaa cgccgccgct gacgccgcca agcgagttta
     1381 ccgcgtttga aacggaaacc ctgcccgttt gcagactttg gcgaatcgct ttttgcgact
     1441 gcggagaaag attttccaaa cctttcacat tgccgttttc gtcgaccgta acctgcccgt
     1501 cgtcgagcgc gagcaaaggt tcgtttttcg ataaattttc cggcgcgggc gaaacatccg
     1561 gcgatgccga attttccggc aaggcgtcga cattcggcga atcgtttgcc gggttgacgt
     1621 ttgaaatcga aatcgtcggc gcgggcgtcg atttattgcc gtttgtttgc gcgatttcgt
     1681 ttttatcgcc gcgcagcaaa aaccacgcgc cgaacaaaac cgccagcaaa atcgccgccg
     1741 cgacgggcgc gaaactgccg attgaatcaa aagcgaagag tttacgccaa aaagattgtg
     1801 tttcagcaga ttttttcggc tcgaccggcg cggcggcaat cgtttgaaac gagcgcaaat
     1861 ccgtcaaatc ttttgagcat tccgcgcaaa tcgcaaaatg gctttcggca atttcccgtt
     1921 cgacatcgtc taattttccg tcggcgaaaa gcgcgagtcg ttcgtacgga agatgctccg
     1981 gctcgtccgc caaatcgtca aacgcgaaat cttcccggag agccgaaaac atttttggcg
     2041 catcgaattt ccgtgccaat ttttcgcggc atatttcaca cgcggaaata tgtttctgca
     2101 cggcgagagt ttccgccgcc gcgagctcgc gtttcccgta ggcgatcaag ctttcttcgg
     2161 taatgtgttc taagttctgc atttatctta tttccgccgt cgccggaaaa acgaaaacgg
     2221 tgttcgtcat taaaatcgag tcggaaacga gcggcttatt accaacatta aaaaaaattc
     2281 ggcaaaaatt ttcgcttttt acgtcacttg cattttcaat caacctcctt tcattctccg
     2341 cgccaatctg tcccgcgccg attttcgcaa attgataacc tgctggcgtg ttaaattcaa
     2401 tttctccgcg atgcgattgt cgtcgagcgg caaatcgttc cacaattcgg caaaggcttc
     2461 cgtgctcatt tccaaaacgg cggcgatttt ttccacgctc gcgacgccga cgacgagcca
     2521 caaatcaatc acgccgcgcc cgttttcgtc ttttagattc agcaaaacgg aggcgcgctg
     2581 gcgcggcggc aaatcggcga tttcattcca caatctttgc agataatcgc gccgctcggt
     2641 ttcggtgtaa gcgtcggggc gcgcgtcggc gagctgcatt tgcgggcgct gttcgccttt
     2701 ttcattcgtg ttcgccgcat cgacctcaat tgtttcgtcc gtaatattcc accaggcggc
     2761 gcaaacgttt accagcaaat caatctcgac gggcgcaccg gtccattcaa aaatcgccgt
     2821 caataactca taagcgtttt tctgctcgct ccaggaattt tgaaccgcca cgtcgcgttc
     2881 aaacgaccgc gcgtcttcac gcagactttg aattttcaaa ctcgaatcgt cacggcttgc
     2941 tccaaattgc cagcgccgaa aaccgccgac ataattttgt ttttcatcct gccagagcgc
     3001 aaaaccttcc cggtgcgcca gcaaataacg caaaccgttt ttcaaacgcc gccgctgcgg
     3061 atatttgcgg ctcaaaaacc ggtcacaggc gttataagtc gtcacggcaa cgtaggaatt
     3121 aaaatcgtcg atcgagctcc cgtttcccac cgctcgcaat tgctgaagcc gccccacaag
     3181 ctgcagcatt acatcgctgt aaatctcttc cccttcttcg tttgcgccgc gtgttttgta
     3241 acgaataatt tttgagacga ccggcgcggc gtgttcggtt aaaagcgcgg acaaagcgcg
     3301 gtcctgctcg tccgcatcgg aagtctgcgc gaaaagacga agcagagaat ctgtttgccc
     3361 ggcattcatt atattgttta tacaatcgaa tgaattttac tcatagtcgg agaggactct
     3421 aaaccaatat tttttgcact ttccggcagc gtatgcttcc attttgagct ttccgccgac
     3481 tgatggttcc gaaaacaaaa agcggcttaa tttaagctgc tcgcaagttg ttgattacac
     3541 ggttgcacag tgacgactta tagtttcgct cgctgccaat ttatggattt aattacaatt
     3601 ttggctgaag cataagcggg agattctgcg atttatgttt ccgaaacact ttgccgactt
     3661 ttgctttctg cggcgggttt gccaagtgat ttttccaaag taagagaccg ctttaaaatg
     3721 atgccgaaaa caaaaagccg cacgaacaga cacgcgcggc ttaataaata gaatttaaat
     3781 tcttgaaaat aaactatgaa tttttctttg ctctggttct tctgggcggt ttggcaggct
     3841 cgccggttat ttcccttttc gccttcagtt tatttccgtc atcaattatt gtaatgaccg
     3901 tcggagtttt cgacctttct attctgaaca tggcttc
//
LOCUS       DASWNE010000771         1656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_95506, whole genome shotgun sequence.
ACCESSION   DASWNE010000771 DASWNE010000000
VERSION     DASWNE010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95506"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..1366
                     /gene="larC"
                     /locus_tag="VGB68_15000"
     CDS             161..1366
                     /gene="larC"
                     /locus_tag="VGB68_15000"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HEY0050598.1"
                     /translation="MRTLYFDCFAGASGNMILGALIALGVGESELIEQIRLLDVSGFN
                     VEVKTVDRSGISAVHADVKVPHEHAHRHLHTIEKIINDSRLSESVKTRAIAIFTRLAE
                     AEAKIHGIDIQKVHFHEVGAMDAIIDVVGACIGFEMLGIEKFACSKIHVGSGFAQMAH
                     GKFPIPPPAVAELLKNAFVYSTEIEGELMTPTGAAIIATVCEEFGRIPEMKIERTAYG
                     AGTREYKGFPNALRLILGESSQPSAISGQPKTEAHRLKTENLILLETNIDDLSPQILG
                     FVMERAFEIGAADCWFTPIQMKKNRPATLLSILCEKDKKEILTEMLFRETSTIGLRVG
                     ETERICLPRRIERIETKFGEIDVKIAGFGGEILNAKPEFDQLKAIAGREKLPLREVEA
                     EVLKSLKNG"
     gene            1359..>1656
                     /locus_tag="VGB68_15005"
     CDS             1359..>1656
                     /locus_tag="VGB68_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050599.1"
                     /translation="MVKPRVKICCISSIAEANLAIRYGASALGLVSEMPSGPGVIALE
                     QIAEIAEKVPPPVGTFLLTCKQTVDEIAAQHEICRTNTIQICDDFVDSYEDLRKA"
BASE COUNT          435 a          386 c          452 g          383 t
ORIGIN      
        1 gtcaggacga tcattactgc gacaagaagt tttaccatta attttttcat tattatttct
       61 cctttaaagg tttagaaaga atggcgtaaa atttggggga aaagtatcag gcggcgacgg
      121 ctttttcttt tccgcgtaaa aaagctagaa ttttatcttt atgcgaacac tttatttcga
      181 ttgttttgcg ggcgcgagcg gcaatatgat tctcggcgct cttattgcgc tcggcgtcgg
      241 cgaaagcgaa ttaatcgagc aaatcaggct gctcgacgtt tccggtttta acgtcgaagt
      301 caaaacggtc gaccgttcgg ggatttccgc cgtgcacgcc gacgtcaaag ttccgcacga
      361 acacgcgcac cggcatctgc acacaatcga aaaaattatt aacgattcgc gtctgagcga
      421 aagcgttaaa acgcgcgcga ttgcaatttt caccaggctt gccgaagccg aagcgaaaat
      481 tcacggcatc gacattcaga aagtgcattt tcacgaagtc ggcgcgatgg acgcgataat
      541 cgacgtggtc ggcgcgtgca tcggtttcga aatgctcgga atcgaaaaat tcgcctgctc
      601 gaaaatccac gtcggcagcg gtttcgcgca gatggcgcac gggaaattcc cgattccgcc
      661 gcccgccgtc gccgagcttt tgaaaaacgc tttcgtttat tcgaccgaaa tcgagggcga
      721 attgatgacg ccgaccggcg cggcgattat cgcgaccgtc tgcgaagaat tcgggcggat
      781 tccggaaatg aaaatcgagc gcacggctta cggcgcggga acgagagaat acaagggttt
      841 tccgaatgcg ctgcgattaa ttttggggga aagcagtcag ccgtcagcaa tcagcggtca
      901 gccgaagacc gaagcgcata gactgaaaac cgaaaacctg attttgctgg aaaccaatat
      961 cgacgattta tcgccgcaaa ttctgggttt tgtgatggag cgagcttttg aaatcggcgc
     1021 ggcggactgc tggtttacgc cgattcagat gaagaaaaat cgtccggcga cgttgctttc
     1081 gattctttgc gaaaaagaca aaaaggaaat tttgaccgaa atgctttttc gggaaacttc
     1141 gaccatcggc ttgcgcgtcg gcgagacgga aagaatttgc ctgccgcgcc gaatcgaaag
     1201 aatcgaaacg aaatttggcg aaatcgatgt aaaaattgcc ggattcggcg gcgaaatcct
     1261 taacgcgaaa ccggaattcg accagttgaa agcgattgcc gggcgcgaaa aactgccttt
     1321 gcgcgaagtc gaagccgaag ttttgaagag cttgaaaaat ggttaagccg cgtgtaaaaa
     1381 tctgctgcat ttcttcaatc gcggaagcga acctggcgat aagatacggc gcttcggcgc
     1441 tcggcttggt gtcggaaatg ccgtccggtc cgggcgttat cgctttggaa caaatcgcgg
     1501 aaatcgccga aaaagttccg ccgccggtcg ggacgttttt gctcacctgc aagcagacgg
     1561 ttgacgaaat cgcggcgcag cacgaaattt gccggacaaa tacgattcag atttgcgatg
     1621 attttgtcga ttcttacgag gatttgcgaa aagcgc
//
LOCUS       DASWNE010000772         4719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_557042, whole genome shotgun sequence.
ACCESSION   DASWNE010000772 DASWNE010000000
VERSION     DASWNE010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557042"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..533
                     /locus_tag="VGB68_15010"
     CDS             42..533
                     /locus_tag="VGB68_15010"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY0050600.1"
                     /translation="MKKYFERCLTLYPTTGFTLFFVGLIPANKFIRKKEIIIMGNTET
                     VKNVKTISTEELAKRLQQNEKFEFWNVLTDEYFQNKLIKGSRRVALDLIGRETANTNL
                     PKDTEIIVYCAGPNCPQSGMAAEKLQTLGYTNVTAYEGGIEEWEAAGLEIEQEKAKSS
                     CCH"
     gene            691..1182
                     /locus_tag="VGB68_15015"
     CDS             691..1182
                     /locus_tag="VGB68_15015"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-responsive transcriptional
                     regulator"
                     /protein_id="HEY0050601.1"
                     /translation="MKKAFSYIQDEQAAGSSGKQLKIGEVSRRSGIGIEALRFYEKSG
                     LLDRPGRTYSGYRLYDESVLERLAFIKQAQVLGFTLDEIKQIITHKQEGESPCAEVRE
                     IVRVRLKELNERIEQMIQYRDDLTAALAEWDETREADGHVCGLIEGAHIEHEISHKKV
                     GKS"
     gene            1257..4040
                     /locus_tag="VGB68_15020"
     CDS             1257..4040
                     /locus_tag="VGB68_15020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEY0050602.1"
                     /translation="MKTEKKLQTAATAATATGEKVVDPICGMTVSPETARGEIEFEGN
                     QYYFCSKGCLETFKRKNNIADSAEQANLMKDGHSPQTAKKETYIDPICGMTVKPETAA
                     AEYEHDGEMVYFCSAGCKTKFVSQLKSQESGNKPAADAHGGHHHHRHHQPEKQIVSHG
                     GMTATPEGEFIDPVCGMSVAPETAAGKYDFDGKTYYFCSTGCQNKFRQNPQNFLEAKK
                     EEKLEAESQGVEYTCPMHPEIVQIGPGSCPICGMALEPKVLTLDDAPDPEYLDMKRRF
                     RISAALTIPVFVLAMAEMLPSFHEIVSPTVSIWIQFVLATPVVLWGGFPFFQRAWASV
                     KNASPNMFTLIAIGTGAAFIFSLFALFTPDLFPVSMRNAHTGLIAAYFEAAAVITTLV
                     LLGQVLELRARSQTSSAIKELLGLAPETAIVVFDDGSEAEIALKDVQIGARLRVKANE
                     KIPTDGVILEGETSIDESMVTGESIPIEKTAGDKVIGGTINGRRGFMMQAEKVGSDTL
                     LAQIVRMVGEAQRSRAPIQRLVDVVSAYFVPAVIVVAIAAFVIWFIFAGFSYALVAAV
                     SVLIIACPCALGLATPMSIMVGTGHGARHGVLVKKAEALEILEKVNTIVVDKTGTLTE
                     GKPRLQKVIALSGFDENEILKFAASLEKSSEHPLAEAIIKGAEERNVEFSKVENFESV
                     TGKGVLGTINGRKILLGNAKLMQENGIDLSQDKNADELRTEGQTVMFVAVDGKFAGLV
                     GVADTIKESAKEAINELRRQKIEVVMMTGDNAKTAEAVAKKLGIDKVFADVLPEQKAE
                     KVKELQAQGRITAMAGDGVNDAPALAQAEVGIAMGTGTDVAMESADITLLKGDLRGIL
                     RAKKLSAATMKNIRQNLFFAFIYNLIGVPIAAGVLYPIFGILLSPMIASAAMTFSSVS
                     VIVNALRLRSLKL"
     gene            4086..>4719
                     /locus_tag="VGB68_15025"
     CDS             4086..>4719
                     /locus_tag="VGB68_15025"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0050603.1"
                     /translation="MNNRDAKILIADDDDSLRRVLEFQLQEAGYTVLTAENGAQALEV
                     FSSRAVDCLVTDWRMPQLSGAELIQRAGVINSETPIIVITAFGDVETAVQAMRGGAFD
                     FITKPFNRQAILLTIEKALKYGQVFAENRRLRRLVHDDFRLESVIGSSEKMRQVFSLV
                     ERVAKTDVTVLIEGESGTGKEIIAKGIHFSGARRDNPFVAVNCAAIPEGLI"
BASE COUNT         1271 a         1091 c         1318 g         1039 t
ORIGIN      
        1 cgctctttct cttcttagtc ggaaaaagca ttttttaacc gatgaaaaaa tattttgaaa
       61 gatgcttgac tctgtatccg actacagggt ttacactgtt tttcgtcggg ttaattccgg
      121 caaataaatt tatcagaaaa aaggaaatca taatcatggg aaatacggaa acagtcaaaa
      181 acgtgaagac aatttcgacc gaagaattag caaaacgtct ccagcaaaac gaaaaattcg
      241 agttctggaa cgttttaacg gacgaatatt ttcagaacaa actgattaaa ggctcgcgcc
      301 gcgtggcgct ggaccttatc ggtcgtgaaa cggcaaatac gaatcttccg aaagatacgg
      361 aaatcatcgt ttactgcgcc ggaccgaact gcccgcagag cggaatggcg gcggaaaaac
      421 ttcaaacgct cggatacacg aacgttacgg cttacgaggg cggcattgaa gaatgggaag
      481 ccgccggact cgaaatcgag caggagaaag cgaagtcatc ctgctgccac taaaaaagtt
      541 aaaagaaggc ttgactctgc atctgactac agagtttata atcaaattaa tggttcagtt
      601 tgaaccaact tgaaaagcga gcggcggagt cggttaaaca gcgattccgc catcatctac
      661 aaaacaaatt tcggagagac gcgagcagtt atgaaaaaag cttttagtta tatacaggac
      721 gagcaagcgg cgggcagcag cggtaaacaa ttaaagattg gggaagtttc gcgccggagc
      781 ggcatcggca tcgaggcatt gagattttac gaaaaaagcg gcttgctcga ccgtcccggt
      841 cggacttaca gcggttatcg cctgtatgac gagagcgttt tggaacgcct ggcttttatt
      901 aagcaggcgc aggttttggg attcactctc gatgaaatca agcagattat cacccacaag
      961 caggaaggcg aaagtccctg cgcggaagtc cgggaaattg tcagagttcg gttaaaggaa
     1021 ctcaacgagc ggattgagca gatgattcaa taccgcgacg acctgacggc ggcgctcgcc
     1081 gaatgggacg aaacccgcga agccgacggg cacgtctgcg ggcttatcga aggagcgcat
     1141 atcgaacacg aaatcagcca taaaaaagtc ggaaaaagct aaattaaagc aaaacaaata
     1201 ttacaaaggc ggcggaatcg ggcgaatgcc gagccgatta aatcgaagga agaattatga
     1261 aaacggagaa aaaattacaa acagcagcaa cagcagcaac agcaacggga gaaaaagtcg
     1321 ttgacccgat ttgtggaatg accgtttctc ctgaaacggc gcggggcgaa atcgagtttg
     1381 aaggtaatca gtattatttt tgctcgaaag gttgtttgga aacctttaaa cggaaaaaca
     1441 acattgccga ttccgcagag caagctaatt taatgaaaga cggtcattcg ccgcaaactg
     1501 ctaaaaaaga gacgtatatc gacccgattt gcggaatgac cgtcaagccg gagacagccg
     1561 ctgccgaata cgaacacgac ggcgaaatgg tctatttttg ttccgccggc tgtaagacca
     1621 agttcgtttc gcaattaaag agccaggaaa gcggcaacaa gcctgcggca gatgctcacg
     1681 gagggcatca ccatcaccgg catcatcaac ccgaaaagca aattgtttcg cacggcggca
     1741 tgaccgcgac gccggaaggc gagttcatcg atccggtctg cggaatgtcg gtcgcgccgg
     1801 aaacggcggc gggaaaatac gatttcgacg gtaaaactta ttatttttgt tcaaccggct
     1861 gccagaataa attccgccag aatccccaaa actttctgga agcaaagaaa gaggaaaagc
     1921 tcgaagcgga aagccagggc gtggaatata cctgcccgat gcacccggaa atcgtgcaga
     1981 taggtccggg aagctgtccg atttgcggaa tggctttaga gccgaaagtt ttgactctgg
     2041 acgatgcgcc cgacccggaa tatctggata tgaagcggcg attccggatt tccgccgcgt
     2101 tgacgattcc ggtttttgtg ctcgcgatgg cggagatgct gccgagtttt cacgagattg
     2161 tttcgccgac tgtttccatc tggattcaat ttgttctggc gacgccggtt gttctctggg
     2221 gcggctttcc gttttttcag cgggcgtggg cttcggtaaa aaatgccagc ccgaatatgt
     2281 tcactttgat tgccatcgga acgggcgccg catttatttt tagtcttttt gctttgttta
     2341 cgcccgactt gtttcccgtc tcgatgcgaa acgcgcacac cggactcatt gccgcgtatt
     2401 ttgaagcggc ggcggtgatt acgacgctcg tccttctcgg acaggttctg gaacttcgcg
     2461 cgcggagtca gacttcaagt gcaattaaag agctgctcgg acttgcgccg gagacggcaa
     2521 tcgttgtttt cgacgacggc agcgaggcgg aaatcgcgct caaagacgtg caaatcggcg
     2581 ccaggctgcg cgtcaaagcc aatgaaaaaa ttccgaccga cggcgtgatt ttagaaggcg
     2641 agacttccat cgacgagtcg atggtgacgg gcgaatcgat tcccatcgag aaaaccgccg
     2701 gcgataaagt tatcggcgga acaatcaacg gcagacgcgg ctttatgatg caagccgaaa
     2761 aggtcggcag cgacacgctt ctggcgcaaa tcgtccggat ggtcggtgaa gcccagcgtt
     2821 cgcgcgctcc cattcagcgt cttgtcgatg ttgtttcagc ttattttgtt ccggcggtta
     2881 tcgtcgtagc gattgccgct tttgttatct ggtttatttt cgccggtttt tcttatgctc
     2941 tggtcgcggc agtatccgtt ttgattatcg cctgcccgtg cgcattaggc ttggcgacgc
     3001 cgatgtcgat tatggtcggc acgggacacg gcgcgcggca cggcgtttta gtcaagaaag
     3061 ccgaggcgct ggagattctg gaaaaggtca acacgattgt cgtcgataaa accggaacct
     3121 taaccgaagg caagccacgc ttgcaaaaag tgatcgcatt atccggtttt gacgaaaatg
     3181 aaattttgaa atttgccgca agtcttgaaa aatcgagcga gcatccgctg gcggaagcga
     3241 ttatcaaagg cgcggaagaa agaaacgtag aattttcaaa ggttgagaat tttgaatccg
     3301 tgaccggcaa gggcgttctc ggaacgatta acggcagaaa gattttgctc ggcaacgcga
     3361 agctgatgca ggaaaatggc attgatttat cgcaggacaa aaacgcggat gagcttcgca
     3421 cggaaggtca aaccgtgatg ttcgtcgcgg ttgacggaaa atttgcgggt ctggtcggcg
     3481 tcgccgacac aatcaaggaa tcggcgaaag aagcgataaa cgagctgcgc cgccagaaaa
     3541 tcgaagtcgt gatgatgacc ggcgataatg cgaaaacggc ggaagccgta gcaaagaaac
     3601 ttggcattga taaagttttc gccgacgttt tgccggagca aaaagcggaa aaggtcaagg
     3661 aattacaagc gcagggcaga atcacggcga tggcgggcga cggcgtgaac gacgcgccgg
     3721 cgctggcgca agccgaagtc ggcatcgcga tgggaacggg cacggacgta gcgatggaat
     3781 cagccgacat cacgctttta aaaggcgatt tgcgcggaat cctgcgggcg aagaaattaa
     3841 gcgcggcgac gatgaaaaac attcggcaga atctgttctt cgcgtttata tacaatctga
     3901 ttggcgttcc gattgcggcg ggagttctgt atccgatttt cggaatactt ttaagtccga
     3961 tgattgccag cgcggcgatg accttcagct ccgtttcggt tatcgtcaac gcgctgcgtt
     4021 taagaagtct aaaattataa actgccagcg ggcgagttta acttttgagg taagataact
     4081 ttaacttgaa taacagagac gcgaaaattt taattgccga cgacgacgat tcactgcgcc
     4141 gggtgctgga atttcaattg caggaagccg gttacacggt tctgacggcg gaaaacggcg
     4201 cccaggcgct ggaggttttt tcgtcgcgcg cggttgactg tctggtcacc gactggcgga
     4261 tgccgcagct ttctggcgcg gaactcattc aacgggcagg cgtcatcaac tcggagacgc
     4321 cgattatcgt gatcacggct ttcggcgatg tcgaaacagc cgttcaggcg atgcgcggcg
     4381 gcgcgttcga ctttattacc aaacccttta accggcaggc gattctgctg acgattgaaa
     4441 aagctctgaa atacgggcag gtctttgccg aaaaccgccg cctgcgaagg cttgttcacg
     4501 atgacttccg gctcgaatcg gtcatcggct cgtcggagaa gatgcggcag gttttcagcc
     4561 tggtcgagcg cgttgccaaa accgacgtca ccgttttaat tgagggtgaa tcgggaaccg
     4621 gaaaagagat tatcgctaaa ggcatccact tttccggcgc cagacgagac aatccttttg
     4681 tagctgtaaa ctgcgcggcg attccggagg gtttaatcg
//
LOCUS       DASWNE010000773         2301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_26311, whole genome shotgun sequence.
ACCESSION   DASWNE010000773 DASWNE010000000
VERSION     DASWNE010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26311"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..1694)
                     /locus_tag="VGB68_15030"
     CDS             complement(30..1694)
                     /locus_tag="VGB68_15030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY0050604.1"
                     /translation="MTKNFFITAIIIVLTGAVLGGIFGKLPARSSADSAVTAEKISGD
                     YREAVEAIGNNYVSKIDHEKVSESSIQGMLWTLDPHSSFFTRDEFRKLYEDQSSKFFG
                     IGVSILQHRDGVYVQSIVPDTPAEKAGLRYGDRFLEVDGKDAKAWTSAEVSKNVRGER
                     GTPVKIRIERAGQEKPVEFEITRDAVPYPSIRNYFLVRNDIGYIGLTGGFQETTAAEL
                     DQALSDLKKQGMKQLVLDLRGNPGGLLPQAVEVVSRFIARGQTVVSVKGRSRYAKSEE
                     LRSVGSRTEDFPLVVLINGGSASASEIVAGAIQDYGRGLIVGSDSFGKGLVQRVFNLP
                     LGTGLTLTTARYYTPFGRSLQRDYSTGSIYDYYTHHGEDDAGKTTPTPDAEPKPQGNP
                     VTTAGGRVFYGGQGISPDLTAPALAFTNLRFRLNEASFFFTRQLISGQIEGLENYRVE
                     RQKFNQSLRPDDFQINDKLLDAFRAFVARDKASGLTAENVNSQLDYAKSRLREEIATA
                     NYSSEAGQQVLLETDPQVLKAIDALPEAAKLARSVKENSMAHLVNK"
     gene            1865..>2301
                     /locus_tag="VGB68_15035"
     CDS             1865..>2301
                     /locus_tag="VGB68_15035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0050605.1"
                     /translation="MKNTLSIVIPAFDESERLGSTVHQILAYIEKQNLSAELIVVDDG
                     SKDNTAAVAEKAFAEFPAIEAKVVRYEKNRGKGYAVKIGLQEAKNDIALFSDADLSTP
                     IEETPKLVELIETGECDVAFGSRALDRSLIGTHQPWRREQGGK"
BASE COUNT          517 a          608 c          626 g          550 t
ORIGIN      
        1 tgctttttct tgtttctttc gggctaactc tatttattaa ccagatgcgc catcgaattt
       61 tccttcacgc ttcgcgccag tttcgccgct tccggcaggg cgtcaatcgc ttttaagacc
      121 tgcgggtcgg tttcgagcaa aacctgctgt ccggcttcgc tcgaataatt ggcggtggcg
      181 atttcctcgc gcagacggga tttggcgtaa tcgagctggc tgttgacgtt ttccgccgtc
      241 aagccgctgg ctttgtcacg ggcgacgaaa gcgcggaaag cgtcgagcag tttatcgtta
      301 atctgaaaat cgtccggtcg cagagattga ttgaatttct gacgctcgac gcggtaattt
      361 tccaagcctt cgatttgtcc ggagatcaac tggcgcgtga agaaaaacga ggcttcgttg
      421 aggcgaaagc gcagatttgt aaaagcgagc gccggcgcgg tcaaatcggg cgaaattccc
      481 tgcccgccgt aaaaaacccg tccgcccgcc gtcgtcaccg ggtttccctg cggcttcggc
      541 tcggcgtcag gcgtcggcgt cgtcttgccg gcgtcgtctt cgccgtgatg cgtgtagtaa
      601 tcgtaaatcg agccggtcga ataatctctc tgaagggagc gtccaaaagg cgtgtaatag
      661 cgcgccgtcg tcagggtcaa gccggttccg agcggcagat tgaaaactct ctgcaccaag
      721 cccttgccga agctgtcgct gccgacaatc aatccgcgac cgtaatcctg tatcgctccg
      781 gcgacgattt ccgaagccga tgccgagccg ccgtttatca aaacgacgag cggaaaatct
      841 tccgtacggc ttccgaccga gcggagttcc tcggatttgg cgtagcgcga gcgacccttg
      901 acggaaacga ccgtctgccc gcgcgcgata aagcggctga cgacttcgac cgcttgcggc
      961 agaagcccgc ccggattgcc gcgcaaatcc agcacgagct gtttcattcc ctgttttttc
     1021 aaatccgaaa gcgcctggtc tagctcggcg gcggtcgtct cctgaaatcc gcccgtcaga
     1081 ccgatatagc cgatgtcgtt gcggacgaga aaataattgc gaatcgaagg atacggaacc
     1141 gcgtcgcgcg taatttcaaa ttcgacgggc ttttcctgac cggcgcgctc gattctgatt
     1201 ttgacgggcg tgccgcgctc gccgcgcacg tttttggaaa cctcggcgct cgtccacgct
     1261 ttggcgtctt taccgtcgac ttccagaaag cggtcgccgt atctcaaacc ggctttttcg
     1321 gcgggcgtgt cgggcacgat tgattggacg taaacgccgt cccggtgctg caaaatcgag
     1381 acgccgatgc cgaaaaattt tgaagactgg tcttcgtaaa gcttgcgaaa ttcgtcgcgg
     1441 gtaaagaacg aggaatgcgg gtcgagcgtc cagagcattc cctgaatcga gctttccgaa
     1501 actttctcgt ggtcgatttt gctgacgtaa ttattgccga tggcttcgac tgcctcacga
     1561 taatcaccgc tgattttttc ggcggtaacg gcgctgtccg ccgaggagcg cgccgggagc
     1621 ttcccgaaga ttccgcccag aaccgccccg gttaaaacaa tgataattgc ggtgataaaa
     1681 aaattctttg tcattactcg ttccgatacc tcttggaaat aatagcataa tcggactttt
     1741 tcgagatagt gaaaagttcc ggtgaaatga ttagatgcaa tgtctcgaaa cgggttttgc
     1801 ccgcactgta tttgttgaaa ataaatttat ctgctatcaa taaactttta attttacaac
     1861 tataatgaaa aacactcttt ccattgttat tccggctttc gatgaatcgg agcgtctggg
     1921 aagcacggtt catcaaattt tagcttatat cgaaaagcaa aacttaagcg ccgaattaat
     1981 cgtggtggac gacggctcga aagataatac ggcggcggtt gccgaaaaag cctttgccga
     2041 atttcccgcg attgaagcga aagtcgtgcg ttacgagaaa aatcgcggta agggctacgc
     2101 cgtcaaaatc ggtcttcagg aagcgaaaaa cgacatcgca ttgtttagcg acgccgattt
     2161 atcgacgccg attgaagaga cgccgaagct cgtcgaattg attgaaacgg gcgaatgcga
     2221 cgtggctttc ggctcgcgcg cgctcgaccg cagccttatc gggacgcatc agccgtggcg
     2281 tcgcgaacag ggcggaaaaa t
//
LOCUS       DASWNE010000774         2822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_274162, whole genome shotgun sequence.
ACCESSION   DASWNE010000774 DASWNE010000000
VERSION     DASWNE010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274162"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1107)
                     /locus_tag="VGB68_15040"
     CDS             complement(<1..1107)
                     /locus_tag="VGB68_15040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050606.1"
                     /translation="MDLAQYRRELKNYYAAVEKNRLEKYTGAAQTIPSPAIAERFSDL
                     FSRAAISDLEKSLENTGELTETETKARQNLLNISRLGFLRSQTEEISAEYESCRQSAR
                     VSFQNENLTFSEALAKIQIEENSAVRREIYARLGEAQAFCADLWLEKFKIRRENAKKI
                     GFENCPKLFEAVAQIDFESFGQKAEKFLVETEEMYFRALSEVFPEAKSLHVADFYFRQ
                     KQSERKEIFDGKKLSNFYRLLLENFNFQPEKISNLKLLEVSGERRTDFFRAGFPSPEV
                     RFAVAARDGNAAFTSFLKTFGKAQQAAWTSADLADRFPEFVFSPDAVLGEAYGILFRN
                     LLADERFLRRNFGLWNEKLTGKIARENNFRLIFEI"
     gene            complement(1344..1652)
                     /locus_tag="VGB68_15045"
     CDS             complement(1344..1652)
                     /locus_tag="VGB68_15045"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY0050607.1"
                     /translation="MFVVLYRWRIKPELEAQFIEAWSEITAYYSRNEHWRGSRLHRGA
                     DGIFYSYAQWESDELRRKAFENRPDFKAGAKMREAIEESFPEVVLEIVADFLILQEKK
                     "
     gene            complement(1764..>2822)
                     /locus_tag="VGB68_15050"
     CDS             complement(1764..>2822)
                     /locus_tag="VGB68_15050"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEY0050608.1"
                     /translation="VVSASHNPFHDNGIKIFSPDGKKIGGATERRIEQDIFDNSKFKI
                     QNSKPEKLDSSKSAEFQNAYLDYLADEFKNLALENFKIVIDCANGASSKLAPRLLERF
                     GAQIVSINCQPDGRNINAGCGSLHIEGLQAKLAAQNADFGAAFDGDADRSLFVDEKGN
                     TVDGDAVLWAMAGHLRAHGKLNHDTVIATVMSNLGLEVALDSLGIKLMRTSVGDKYVL
                     EELLKTNASIGGEQSGHIIFPFRSLVGDGMLTMLFLLEAMQESGKTLSELTAGFVRFP
                     QVLVNVKVKDKRPFDDVGEISMISKQVEAELGQKGRLLLRYSGTENLARVMIEGENQH
                     QIESQAHRLAEVIKASLG"
BASE COUNT          691 a          713 c          672 g          746 t
ORIGIN      
        1 aatttcaaaa atcagccgaa aattattttc ccgcgcgatt tttccggtca gtttttcatt
       61 ccacaaaccg aaatttcgcc gcagaaacct ttcgtccgcc agcagattac gaaacaaaat
      121 gccgtaagcc tcgcctaaaa ccgcgtccgg cgaaaaaacg aattcgggaa aacgatcggc
      181 taaatcggcg gatgtccaag ccgcctgctg agcttttccg aacgttttta aaaacgatgt
      241 aaaagccgcg tttccgtcgc gcgcggcgac ggcaaaacga acttccggcg acggaaaacc
      301 ggcgcgaaaa aagtctgtcc gtctttcgcc ggaaacttcc agaagcttaa gatttgaaat
      361 tttctccggc tgaaaattga agttttcgag cagcagacga taaaaattcg acagtttttt
      421 tccgtcgaaa atttctttcc gttccgattg cttttgccgg aaataaaaat cggcgacgtg
      481 cagggatttt gcttcgggaa aaacttcgga aagcgcccgg aaatacattt cttcggtttc
      541 gacgagaaat ttttcggctt tttgcccaaa actttcaaaa tcaatttgcg cgacggcttc
      601 aaacaatttc gggcagtttt caaagccgat ttttttagcg ttctcgcgcc ggattttaaa
      661 tttttccagc cataaatcgg cgcagaaagc ttgcgcttcg ccgagccgcg cgtaaatttc
      721 gcggcgcacg gcggaatttt cttcaatttg aattttggcg agcgcttcgg aaaaagtcag
      781 gttttcgttt tgaaaagaaa cgcgcgccga ttggcggcag gattcgtatt cggcggaaat
      841 ttcttcggtt tgactacgca gaaaaccgag ccgcgaaata ttcagcagat tttgccgcgc
      901 ttttgtttcc gtctcggtta gctcgccggt gttttccagc gatttttcga ggtcggaaat
      961 cgccgcccgc gaaaacaagt cgctaaagcg ctcggcaatc gccggcgacg gaattgtctg
     1021 cgccgcgccg gtatattttt ccagccgatt tttctcgacg gcggcataat aatttttcag
     1081 ttcgcggcgg tattgagcca aatccatttt ttgagattaa accgaaatgc attttggaag
     1141 caaaatcaaa gagccgcaag tttttagctt gcggctctta aaaagagtgg gcaggcgaag
     1201 caacgtggaa gtgcgccgcg cctgccaatg cgtgattact gtcctcaccc cagcaaccac
     1261 tagagacgga ggctccacca accgtctcga ttgaagctac tatacaacta tttttatttc
     1321 gattgcaaga agaaaaatgg attttatttt ttttcttgca aaatcagaaa atcggcgaca
     1381 atttccagaa cgacttccgg aaaactttcc tcgattgcct cgcgcatttt cgcgccggct
     1441 ttgaaatcgg gtcgattttc aaacgccttt cggcgcagct cgtccgattc ccattgcgcg
     1501 taggaataga aaattccgtc tgcgccgcga tgcagacgcg agccgcgcca atgctcgttt
     1561 cggctgtaat aggcggtaat ttccgaccac gcttcgataa actgcgcttc cagttccggc
     1621 ttgattcgcc atcgataaag cacgacaaac ataattttat aaaagcgaaa aagtaaaaaa
     1681 gtaaaaaagt gaaaaggtaa ttgctgtttt tcgccagcca ctttttcaca ctttcacttt
     1741 ttcacacttt cactttttca ctttcatccc agacttgctt taatgacttc cgccaggcgg
     1801 tgcgcctgcg attcgatttg atgctggttt tcgccttcaa tcatcacgcg ggcgagattt
     1861 tccgtgccgg aatagcgaag cagaagtctg cccttttgac cgagctcggc ttcgacctgt
     1921 tttgaaatca tagaaatttc gccgacatcg tcaaacgggc gtttgtcttt gactttcaca
     1981 ttgaccaaaa cctgcgggaa acgaacgaat ccggcggtca gctccgacaa ggtttttccg
     2041 ctttcctgca tcgcttcgag cagaaaaagc atcgtcagca ttccgtcgcc gaccagcgaa
     2101 cgaaacggaa aaattatatg accggactgt tcgccgccga tgctggcatt tgttttcaga
     2161 agctcttcga gaacatattt atcgccgacc gacgtgcgca tcagtttgat gccgagcgaa
     2221 tcgagcgcga cttccagacc gagattgctc atcacggtgg cgataaccgt atcgtgattg
     2281 agcttgccgt gagcgcgcag atgtccagcc atcgcccaca aaaccgcgtc gccgtcgacc
     2341 gtatttccct tttcgtcaac aaacagcgag cggtcggcgt cgccgtcgaa agccgcgccg
     2401 aaatcggcgt tttgagccgc gagcttagcc tgcaagccct cgatatgaag cgagccgcag
     2461 ccggcgttaa tattgcgccc gtccggctga caatttatcg acacgatttg cgcgccgaat
     2521 ctttccagaa ggcgcggcgc gagctttgaa gaagcgccgt tggcgcagtc aatcactatt
     2581 ttgaaatttt cgagcgcgag atttttgaat tcgtcggcga gataatccag atatgcgttt
     2641 tgaaattcgg cggattttga cgaatcgagc ttttccggtt tcgagttttg aattttgaat
     2701 tttgaattgt cgaaaatatc ctgttcgatg cgccgctcgg tcgcgccgcc gatttttttt
     2761 ccgtccggcg aaaaaatttt gatgccgttg tcgtgaaagg ggttatgcga tgcgctgacg
     2821 ac
//
LOCUS       DASWNE010000775         2965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_622468, whole genome shotgun sequence.
ACCESSION   DASWNE010000775 DASWNE010000000
VERSION     DASWNE010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622468"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VGB68_15055"
     CDS             complement(<1..311)
                     /locus_tag="VGB68_15055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050609.1"
                     /translation="MRLTKAVLLLMLVFNLSANFFAQTKTPDDTDEIIRVDTQLVDVP
                     VVITDKTGKPLLNLKQNNFVIYEDGKPQEISDFVATAAPFEVALLLDTSGSTRSDLPL
                     I"
     gene            632..2209
                     /locus_tag="VGB68_15060"
     CDS             632..2209
                     /locus_tag="VGB68_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050610.1"
                     /translation="MVSVFANFTLFVSANTTPQPLPFTQNWTNTGLITANDDWTNVPG
                     IVGYLGDIGTTTQANLDPRAITQDFATQDVIANQTNPDTQASGGVAEFEITNPTIALQ
                     GSGTADAPNIVIYLNTTGQSNIRVTFNARDIDASMDDAVQQLAVQYRVGTTGNFSNVP
                     GPGAYFADVTTTATATQVTPVAVTLPPTANNQPNVQVRIITTNAAGSDEWVGIDDINI
                     TGGFAAPNIFRAESDFNGDGRTDYAVTRGLATLKQWIYSFAPNTEANVVQFGNLTDRE
                     VPADYDGDGRTDIAVYRPAAAPNSFFYVLQSSNSTLLYNELGASGDDPTIVGDYTGDG
                     KADMAVYRASGGQGSFFYRPSEAPGGIVFVPFGSGTTTRAIPGDYDGDGRFDFCVRFN
                     GGNNQAVFAVARSTGGTSYTQWGLMSDSVAPGDYDGDLRWDFTALRPTSGNYFWYVLE
                     ADGGTFGVQWGLTTDTQVPGDYDGDGRTDIAVWRGSVDPTQNFYYFRQSSANFALGAV
                     EFGQVGDYPVQNFRVRQ"
     gene            complement(2328..>2965)
                     /locus_tag="VGB68_15065"
     CDS             complement(2328..>2965)
                     /locus_tag="VGB68_15065"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY0050611.1"
                     /translation="PRAMSAEEARQFQGLILDVRNADEYGAAHISGSLNIGLGGQFAS
                     WAGTLIESGAPLVIVAESEEKTDEAVMRLSRVGIESVEGFLKGGIEAWKNAGFEVKTI
                     EQVSVREAQKRLAATPSAQFVDVRRGGEYESGHAPEALNLTLSDLDKLTAKLDQSRPT
                     YVICQGGYRSSAATSILERKGFRELYNIAGGTAAWIKEGLETEAETKSQAA"
BASE COUNT          670 a          843 c          766 g          686 t
ORIGIN      
        1 tgaatcagag gcaaatccga gcgcgtcgag cctgaagtgt cgagcagcag agcgacttcg
       61 aacggcgcgg cggtggcgac gaaatcagag atttcctgcg gcttgccgtc ttcgtaaatg
      121 acgaaattgt tttgtttcag attcaaaagc ggcttgcccg ttttgtccgt gataacgacc
      181 ggaacatcga ccaattgcgt atcgacgcgg ataatttcgt ccgtatcatc cggcgttttc
      241 gtctgcgcga aaaaatttgc cgaaagatta aaaaccagca tcaacagtaa aacagctttc
      301 gtcaaacgca tctttaacct ctcaaaacgg aatttgctta cgattttagc agaaagcgtc
      361 cgccagacaa gcgcccggcg gacggacggg aaaatttatt gtcaaagccg gaaaattttc
      421 cgaaaaaaaa gcggtttccg aaaaattcgt taacgttttt ctaacttttc tatgttattc
      481 tctttgtaaa tcgaattgta aatcgaaaaa catttcactt cggacaaagc ttccgcttcc
      541 gcgaagaacg catttgtctt gagatttttt gaagttttaa gaggagaaaa ggatgagaag
      601 gattattttt tttagcgttt ccgctttact aatggtaagc gtgtttgcaa actttacatt
      661 gtttgtttca gccaacacca cgccgcagcc attgccgttc acacaaaact ggacgaacac
      721 cggattgatt accgctaacg acgattggac aaatgttccg ggcatcgtcg gctatctggg
      781 tgatatcggg actaccacgc aagccaatct tgatccgcgc gccataacgc aggacttcgc
      841 cacacaggat gtgattgcca accagacgaa ccccgacacg caggcatcgg gcggcgtggc
      901 ggaatttgaa atcaccaacc ccaccatcgc tctgcaaggc tcaggcacgg ctgacgctcc
      961 gaacatcgtt atttatctga ataccacagg acagagcaat atccgcgtga ctttcaacgc
     1021 ccgcgacatc gacgcttcga tggacgacgc cgtccagcag ctcgccgttc aataccgcgt
     1081 cggcacgacg ggaaatttca gcaacgttcc cggtccgggc gcttatttcg ccgacgtgac
     1141 gaccaccgct acggcgacgc aggtcacgcc ggttgccgtt accctgccgc cgacggcgaa
     1201 taatcagccg aacgtgcagg tgcgcattat aacgaccaat gcggcgggca gcgacgaatg
     1261 ggtcggcatc gacgacatca acatcacggg cggcttcgcc gcgccgaaca tctttcgcgc
     1321 cgagtcggac tttaacggcg acggcagaac cgactatgcg gtgaccagag gcttagccac
     1381 tctgaaacag tggatttaca gctttgctcc caacaccgag gcgaatgtcg tgcaattcgg
     1441 caacctgacc gaccgtgaag ttccggctga ctatgacggc gacggcagaa ccgatatcgc
     1501 ggtttatcgt ccggcggctg cgccaaactc cttcttctac gtgctgcaaa gttcgaacag
     1561 tacgcttttg tataacgagc tcggcgcttc cggcgacgac ccgacaatag tcggcgatta
     1621 taccggcgac ggcaaagccg atatggcggt gtatcgcgcc agcggcggac agggctcttt
     1681 cttctatcgt ccgagcgaag ctccgggcgg cattgttttc gttcccttcg gcagcggcac
     1741 tacgaccaga gccattccgg gcgactatga cggcgacggc agattcgact tctgcgtgcg
     1801 cttcaacggc ggcaacaatc aggcggtttt cgccgtggct cgctcgaccg gcggaaccag
     1861 ctacacccag tggggactga tgtccgattc ggtcgcgccg ggcgattacg acggcgacct
     1921 gagatgggac tttaccgcac ttcgcccgac cagcggcaac tatttctggt atgttctgga
     1981 agccgacggc ggaaccttcg gcgttcaatg gggattgacc accgacacac aagtgccggg
     2041 cgattacgac ggcgacggca gaaccgacat tgcggtctgg cgcggttcgg ttgacccgac
     2101 gcagaatttc tactacttca gacaatcgtc ggcaaacttc gctctcggag cagtcgaatt
     2161 cggacaagtc ggcgattatc cggtgcagaa tttccgcgtt cgccagtagc tgaaattcag
     2221 ccaaatgcaa aatcggagag attttttcag gtctctccga ttttttgttt ttatccgcga
     2281 aaatctgcgg ctgatttctt cttaatagtt tacgccgcct gcatatttta cgccgcctgc
     2341 gatttcgttt ccgcttccgt ctccaagcct tctttaatcc acgccgccgt cccgcccgcg
     2401 atattgtaaa gctctcgaaa accttttcgc tctaaaatgc tcgtcgccgc gctggagcga
     2461 taaccgccct gacagatgac gtatgtcggg cgcgattggt cgagctttgc ggtcagttta
     2521 tccaaatccg ataaggttaa attcaatgct tcgggcgcgt gcccgctttc gtattcgccg
     2581 ccgcggcgca cgtcgacaaa ctgcgcggac ggcgttgcgg caaggcgttt ttgcgcctcg
     2641 cggacggaaa cctgctcgat ggttttcact tcaaaaccgg cgtttttcca cgcttcgatt
     2701 ccgcctttca gaaatccttc gaccgattcg atgccgacgc gcgacaggcg catcacggct
     2761 tcgtccgttt tttcttccga ttcggcgacg atgaccagcg gcgctccgct ttcaatcagg
     2821 gttcccgccc acgaggcgaa ttgcccgcca agcccgatat tcaaagagcc ggaaatatgc
     2881 gccgcgccgt attcatcggc atttcgcacg tctaaaatca agccttgaaa ttgtcgagct
     2941 tcttctgcgc tcatcgctcg cggct
//
LOCUS       DASWNE010000776         1676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_377247, whole genome shotgun sequence.
ACCESSION   DASWNE010000776 DASWNE010000000
VERSION     DASWNE010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377247"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="VGB68_15070"
     CDS             complement(<1..1059)
                     /locus_tag="VGB68_15070"
                     /inference="COORDINATES: protein motif:HMM:NF019156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050612.1"
                     /translation="MPVNSFVDKFFKKVLGSNSDVFLKKVKPTVAEINSIEPELENLS
                     DDELRAKTAQFKEEIQNAIAGIEDKKERRKREQEVLHEILPEAFALVREASRRVTGMR
                     HFDVQLIGGVALHQGRIAEMRTGEGKTLVSTLPAYLNGLTGRGVHIVTVNDYLALRDA
                     EWMGKIHTFLGLTVGCIQNDMDDYERKEAYACDITYGTNNEFGFDYLRDNMKFDVESL
                     VQRDHYFAIIDEVDSILIDEARTPLIISGASDEATDKYFVANEIIPKLVRGHKDEETK
                     VTTGDYLLDEKNHSAVLTEQGVDKAEKLLGVSNLYDPANMDLLHCVEQALKAHTLYKV
                     DHHYVVQEGEVIIVDDFTG"
     gene            complement(1179..>1676)
                     /locus_tag="VGB68_15075"
     CDS             complement(1179..>1676)
                     /locus_tag="VGB68_15075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050613.1"
                     /translation="SIEKFNFGYSEFQFKRNYFSLCDANGKPRGIAQAETNDSRILSS
                     WVSAAVTDAVYKFEDWNKFSDCLPNGKIEKSNAESFSTKISYDKAGGLKVELIIDKDS
                     KQSAEHLAGSFNQAFKAYEVEQDEPIVKEISKNTSFKSENNQIFIVTRLPRGSIDSLL
                     AKNAN"
BASE COUNT          368 a          407 c          411 g          490 t
ORIGIN      
        1 cccggtgaaa tcgtcgacga tgatgacttc gccttcctgc acgacataat gatggtcgac
       61 tttgtagagc gtgtgcgctt tcagagcctg ctcgacgcag tgcagcaaat ccatattcgc
      121 cgggtcgtag agattggaaa cgccgagcag cttttccgct ttgtccacgc cctgctcggt
      181 taaaaccgcc gagtgatttt tttcgtccaa aagataatcg ccggtcgtga ctttcgtttc
      241 ttcgtccttg tgaccgcgca cgagtttggg aataatctcg ttggcgacga aatatttgtc
      301 ggtcgcttcg tccgaggcgc cggaaataat cagaggcgtt ctggcttcgt caatcaaaat
      361 cgagtcgact tcgtcgataa tcgcaaaata atggtcgcgc tgaaccaggg attcaacgtc
      421 gaatttcata ttgtcgcgca ggtaatcgaa gccgaattcg ttgttcgttc cgtaggtgat
      481 gtcgcaggcg taggcttctt tgcgctcgta atcgtccata tcgttctgga tacagccgac
      541 cgtcaagcct aaaaatgtat ggattttgcc catccattcc gcgtcgcgca gagcgagata
      601 atcgttgacc gtgacgatgt gaacaccgcg tccggtcaag ccgttcagat aagcgggcaa
      661 ggtcgaaacc agcgttttac cttcgcccgt tcgcatctcg gcgattctgc cctgatgcag
      721 ggcgacgccg ccgatcagct gaacgtcgaa atggcgcatt ccggtcacgc ggcgcgaggc
      781 ttcgcgcacc agagcgaagg cttcgggcag aatttcgtgc aggacttcct gctcgcgctt
      841 gcggcgctcc ttcttgtctt cgataccggc gatcgcattt tgaatttctt ctttaaactg
      901 cgccgttttc gctcttaatt catcgtccga aagattttcc agttccggct ctatggagtt
      961 aatttccgcg acggtcggtt tgaccttttt cagaaacacg tcgctgttgc tgccgagaac
     1021 ttttttaaaa aatttatcta caaaactgtt tacaggcata aattcctcat ctgtcgagct
     1081 gaaaagcaaa tcgctattag ctcataaaat tgttaattat taaagttcag gcgagttttt
     1141 ttaggaaaga ttggattcgt tcgcttgcct ctcgccgcct agtttgcgtt tttggcgaga
     1201 agcgaatcta tcgagccgcg cggcaggcgg gtgacgatga aaatctggtt attttcagat
     1261 ttaaaggaag tgtttttgga tatttctttg acgattggct cgtcctgttc aacttcgtaa
     1321 gccttgaacg cctgattaaa gcttccggcg agatgttcgg cagattgttt cgagtctttg
     1381 tcaataataa gctcgacttt taagccgcct gccttgtcat aactgatttt agtgctgaag
     1441 ctttcggcgt tgcttttttc aattttgccg tttggcaaac aatcggaaaa tttattccag
     1501 tcttcaaact tataaaccgc atcagtcacc gcggcggaaa cccacgaact gagaatccgc
     1561 gagtcattcg tttcggcttg tgcgatgccg cgcggttttc cgtttgcgtc gcagaggcta
     1621 aagtaatttc ttttgaattg aaactcgctg tagccaaagt tgaatttttc aatgct
//
LOCUS       DASWNE010000777         6190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_377268, whole genome shotgun sequence.
ACCESSION   DASWNE010000777 DASWNE010000000
VERSION     DASWNE010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6190
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377268"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..508
                     /locus_tag="VGB68_15080"
     CDS             53..508
                     /locus_tag="VGB68_15080"
                     /inference="COORDINATES: protein motif:HMM:NF024297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0050614.1"
                     /translation="MKKFTVLFILVAVGSLFYGYGVWADEKEKDAARVPLENYLKGHA
                     SGNPEFMRKAFHTEGKLIFIREGKYSTRTFEEYINGMKDGKPAADEAQRKRWIESVEV
                     SGNAGVGKIILDYPSGKFVDYMTLLKIDGEWKIVNKSFHFEPKAQPAAK"
     gene            686..1768
                     /locus_tag="VGB68_15085"
     CDS             686..1768
                     /locus_tag="VGB68_15085"
                     /inference="COORDINATES: protein motif:HMM:NF025854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY0050615.1"
                     /translation="MSLFNFKNKFPSFWTIQLAGWIVYFVTIYITFLTVAQPGNFLNL
                     FYLKGFRALTGFFLTSVLMRPIYRRFGNRLSIGWLVFLVLICASVFGYIWTGIEFCYA
                     YLTNPVLNLDNYLARSPRIALDYAMTLTAWSALYLGVKSWLSWQTERENALQSSALAQ
                     KAQLEMLRYQLNPHFLFNALNSIRASVDEDSTRAKQMITQLSEFLRYSLLNETARKIS
                     LRDEMEAVRNYLAIEKIRFEDKLAIEFDVETRAENFKVPCFLLNPLVENAIKHGLQTS
                     PKPLKIKISARVEANKLFLEISNTGNLQNNPSRNGNGIGLKNVRERLENLPDEKGKFE
                     LLQENGLVKARIEIAKLGKLTTDERG"
     gene            1925..2641
                     /locus_tag="VGB68_15090"
     CDS             1925..2641
                     /locus_tag="VGB68_15090"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY0050616.1"
                     /translation="MKYKAIIIDDERLARKEMHGLLSEFDEISVAGEAENLTEAINLI
                     EKEKPNIVFLDIQLSGENGFDLLEKIERNFKLVFVTAFDAYAIRAFEVNALDYLLKPV
                     NPERLAKAIERLSEENTAKNDFRPLEFDDRIFLELGARSVFLKVRDISHINAAGDYSE
                     IFTVDKRKLLIEKSLREWEERLPEKNFLRIHRQTIVNLEEIEHIESWFNRTFQIRLKN
                     YRESFPVSRRYSVKLKNRFN"
     gene            2644..3225
                     /locus_tag="VGB68_15095"
     CDS             2644..3225
                     /locus_tag="VGB68_15095"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEY0050617.1"
                     /translation="MLKAELRKIYLARRKSLPAKERGEKSRRIAAVFFQNFDLSKIRY
                     LHCFLPIEKFGEIETEPIFRRVWLDFPQIETLVPRVDFRADEIENLKFTSATALVRNA
                     WQIDEPAHDESVEPEKIDLVLVPLLCFDEKGFRVGYGKGFYDRFLKSCRPDCLKIGLS
                     YFSPVDEIEDAQNHDVKLDFCVTPERIFSHREL"
     gene            complement(3338..3793)
                     /gene="mscL"
                     /locus_tag="VGB68_15100"
     CDS             complement(3338..3793)
                     /gene="mscL"
                     /locus_tag="VGB68_15100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEY0050618.1"
                     /translation="MWKEFRAFIARGNILDLAIAVIMGAAFSSIVKTFTDGIIMPFVG
                     VLAGKIDFKDKVWNLGDMPVTTADDIVRAQAAKQPLIMYGQFINDIINFLIVAFVMFL
                     LARYAMKLFKRFEAAATPPTGEEVLLAEIRDILKAREFSAAGSTQGKRE"
     gene            4084..4293
                     /locus_tag="VGB68_15105"
     CDS             4084..4293
                     /locus_tag="VGB68_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050619.1"
                     /translation="MKDLLYAIVALIAVGIAIWQFIVFIGQPPRNVSYTPIIIAGICA
                     LIALVCGGLFLSGRVNKTDDIHITE"
     gene            complement(4401..5336)
                     /locus_tag="VGB68_15110"
     CDS             complement(4401..5336)
                     /locus_tag="VGB68_15110"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY0050620.1"
                     /translation="MLKQISFCLVLMLLFSVFVFAQKKTTPLKSNAVLPSGAPPTLDE
                     RINAAIRDFKGKVWIYAKNLDTRETFSLRGGERVRTASTIKLPIMVETYFQINEGKLR
                     WTDEIVLTKEKKVGGSGILGEFSDNTKIDLRTAVNLMIVVSDNTATNLVLEKVTSDAV
                     NARMESLGFTDTKSIRRIFGSEKDSRVAADPVIKNFGLGMSSPKDMVGILEMLELGQL
                     INAEASKSMLDVLRRQQYKDGIGRNALDTVPVASKSGTLDRLRADVGIVYTRRGRIAM
                     AITVDDMQVVHYATDNPGSLLIWQLSQILQEGLAR"
     gene            5608..6171
                     /locus_tag="VGB68_15115"
     CDS             5608..6171
                     /locus_tag="VGB68_15115"
                     /inference="COORDINATES: protein motif:HMM:NF020295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEY0050621.1"
                     /translation="MPKIADIQETPNPNAVKFILKEPVSHGTSHSFKTAEDAADNALA
                     KSIFDIGNVVSVFYMDKMITVEKTDEADWDEILPTLAVPIRAAEAVSVTNGNGAAKSV
                     GGAIAAALDDNPQVREIEALLDERIRPYLASDGGWLEIVGLEDKTLRIRYEGACGSCP
                     SSLTGTLMAIENMIKEEIDPEISVITV"
BASE COUNT         1648 a         1444 c         1490 g         1608 t
ORIGIN      
        1 ccgggtttat cgacgattaa ttttcaggaa ataaaatcaa ggagcaatac tgatgaaaaa
       61 atttacggtt ttatttattt tggtcgccgt cggcagcctg ttttacggct acggcgtctg
      121 ggcggacgaa aaggaaaagg atgcggcgag agttccgctc gaaaattatt tgaagggaca
      181 cgcctcggga aatcccgaat ttatgcgcaa agcttttcac acggaaggca agctgatttt
      241 tatccgcgaa ggcaaatact cgacgcgcac tttcgaagaa tacatcaatg gaatgaagga
      301 cggaaaaccc gcggcagacg aagcgcagcg caaacgctgg attgaaagcg tcgaagtttc
      361 gggaaacgcc ggcgtcggca agattattct cgattatccg agcggaaaat ttgtcgatta
      421 tatgacgctt ttgaaaatcg acggcgagtg gaaaatcgtc aacaagagtt ttcatttcga
      481 gccgaaagcg caaccggcag ctaaataatc cgagctggac gtaaaacgga aactgaagag
      541 atagcccgcg aaagcgcacg aaaaacgcgc gaaaaagatt aagaaaaatt atttaatctt
      601 tgcctttgtt tttattttcg cgttttttcg tgcgctctcg cggacaaaat cttttaaagt
      661 tttataatcg gcggtttgtt taaaaatgtc cctgttcaat tttaaaaata aatttccgtc
      721 attttggaca attcagctgg ctggctggat tgtttatttt gtgacgattt acattacctt
      781 tctgacggtc gcgcagccgg gaaattttct gaatctgttt tatctgaaag gttttcgcgc
      841 gctgaccgga ttttttctga cctcggttct gatgcgcccg atttaccgtc gtttcggaaa
      901 ccggctttcg attggctggc tggtttttct ggtcttgatt tgcgcttcgg ttttcggcta
      961 catctggacg ggaatcgaat tttgctatgc gtatctgaca aatccggttt tgaatctgga
     1021 caattatctc gcccgcagcc cgcgcatcgc gctcgattac gcgatgactc tgacggcttg
     1081 gagcgctttg tatctgggcg taaaaagctg gctgtcgtgg cagacggagc gcgaaaacgc
     1141 tttgcaatct tcggctctgg cgcaaaaggc gcagctggaa atgcttcgtt accagctaaa
     1201 tccgcatttt ctttttaacg ctttaaattc aattcgcgcc tcagttgacg aagactcgac
     1261 gcgcgccaag cagatgatta cgcaattgtc cgaatttctg cgctattcgc tgctgaatga
     1321 aaccgccaga aaaatttctc tgcgcgacga aatggaagcc gtgcgaaatt atctggcgat
     1381 tgagaaaatt cgttttgaag ataaattagc gatcgagttt gatgtcgaaa cgcgcgcgga
     1441 aaatttcaaa gtcccgtgtt ttctgcttaa tccgctggtc gaaaacgcga taaaacacgg
     1501 tttgcaaacc agcccgaaac cgctgaaaat taaaatctcg gcgcgcgtcg aggcgaacaa
     1561 attgtttctg gaaatctcga acacgggaaa tctgcaaaac aatccgagcc gaaacggaaa
     1621 cggcatcggt ctgaaaaacg tgcgcgagcg gctcgaaaat ctgccggacg aaaaaggcaa
     1681 attcgagctg ttgcaggaaa acggtctggt gaaagctcgg attgagattg ccaagctagg
     1741 gaaattaacc acggatgaac gcggataaac gcagattcga acaggatgga cagaatacgc
     1801 aggataaaaa acgaaactcg ctgcttgctc ttaaattatc ctgtccatcc cgttaaatta
     1861 tccgcgttta tccgtgtaaa tctgtggctg aattctctct ttccctctgc ggctgattaa
     1921 aaaaatgaaa tacaaagcaa tcatcatcga cgacgagcgt ctggcgcgaa aggagatgca
     1981 cgggcttttg tcggaatttg atgaaatcag cgtcgccggc gaagccgaaa atctgacgga
     2041 agcaattaat ttaattgaaa aggaaaagcc gaacatcgtt tttctggaca tccagctttc
     2101 cggcgaaaac ggctttgatt tgctcgaaaa aatcgagcgg aatttcaaac tggttttcgt
     2161 cacggcgttt gacgcgtatg cgattcgcgc ttttgaagtc aacgcgctcg attatctttt
     2221 aaagccggtt aacccggagc ggctggcaaa agcgattgag cgattaagcg aagaaaatac
     2281 tgctaaaaac gattttcgcc cgctcgaatt cgacgaccgc atttttctcg agctcggcgc
     2341 gcgctccgtg tttttaaaag tccgggacat ctcgcacatc aatgcggcgg gcgattattc
     2401 cgaaattttt acggtcgata aaagaaagct tctgattgaa aaatctctgc gcgagtggga
     2461 agagcgcctg ccggaaaaga attttttgcg gattcatcgt caaaccatcg ttaatcttga
     2521 agaaatcgaa cacatcgaat cgtggtttaa tcgcacgttt cagattcgcc tgaaaaatta
     2581 ccgcgaaagc tttccggtca gccgccgcta ttcggtcaag cttaaaaaca ggtttaatta
     2641 aaaatgctga aagccgagct ccgcaaaatt tatctcgccc gccggaaatc gctcccggcg
     2701 aaagaaagag gcgagaaaag ccgccggatt gcggctgttt tttttcaaaa ttttgatttg
     2761 agcaaaatcc ggtatctgca ttgttttctg ccgattgaaa agttcggcga aattgagaca
     2821 gagccgattt tccgccgggt atggctggat tttccgcaga ttgaaactct cgttccgcgc
     2881 gtcgatttcc gcgccgatga aatcgaaaat ttgaaattta cgtccgcgac cgcgctcgtc
     2941 cgaaacgcct ggcagattga cgagccggcg cacgacgaat cggtcgagcc cgaaaaaatc
     3001 gatttggttc tggttccgct tttgtgcttt gacgaaaaag gctttcgcgt cggttacggc
     3061 aaaggttttt atgaccgttt tttgaaaagc tgccgccccg attgtttaaa aatcggtctg
     3121 agttattttt cgccggtcga cgaaatcgaa gacgcgcaaa accacgacgt aaagctcgat
     3181 ttttgcgtca cgccggaaag aattttcagc cacagagagc tttaagagaa aagccgcaaa
     3241 ttcacgcgga caaacgccgc cgaggagaaa gattttagct gttaattatg tcaatcaagc
     3301 cgcgaagcct gatttgaatc cgcgtttatc cgtcgtttta ttctctcttt ccctgtgtgc
     3361 tcccggcggc tgaaaattct cttgctttca aaatgtcgcg tatttccgcc agcagaactt
     3421 cctcgcccgt cggcggcgtt gccgcggctt caaaccgctt gaaaagcttc atagcataac
     3481 gcgccagcaa aaacatcacg aaagccacta tcaaaaaatt gataatgtcg ttgataaatt
     3541 gcccgtacat aatcagcggt tgttttgccg cttgcgccct gacaatgtcg tcggcggtcg
     3601 ttaccggcat atcgcctaaa ttccaaactt tatctttaaa atcgattttg cccgccagca
     3661 cgccgacaaa cggcattata attccgtcgg taaaagtttt gacaatcgag ctgaaagccg
     3721 cgcccataat cacggcaatc gccaaatcga ggatatttcc gcgcgcgata aacgctctaa
     3781 attctttcca catatttatt agtccctgtc gtttattttg tttcgttaga ttgaaaaagc
     3841 ataaaatatt tatcttatag cccggcgcgc gtcaaggatt acgtctgaaa taaaagccgt
     3901 tttataaaaa aatcttgcct tttcgataaa aatagcgtta ctattgattg cattccataa
     3961 agcagtttga aagtctaaaa ataattttcg aggctcggtt tgccaacgcc gggttgtggt
     4021 cataaatcca gtagtacttt ggaaggcaag caagcccttc ctcgaaaacg gaggtgattg
     4081 attgtgaagg atttgctgta cgcaattgta gcactgattg ccgtcggtat cgctatctgg
     4141 caatttattg tttttatcgg gcagccgccg cgaaacgtca gttatacgcc gataatcatt
     4201 gccgggattt gcgcattgat tgcgctggtt tgcggcggtc tgtttttatc gggcagagtc
     4261 aacaaaactg acgacattca tattaccgaa taacttcggc taatttgcga tgagcggtca
     4321 gactggaact ttccgctcat catcttcgaa aacctgtttt taacaggata gagttttact
     4381 gcgcccgaaa tccgtttttc ctacctcgcc aagccttcct gcaaaatctg cgacagctgc
     4441 cagattaata agcttcccgg attgtccgtc gcgtaatgca cgacctgcat atcgtcaacc
     4501 gtaatcgcca tcgcgattcg cccgcgccgc gtgtagacga tgccgacatc cgcgcgcagg
     4561 cggtcgagcg ttcccgattt tgaagcgacc ggaaccgtgt ccagagcgtt gcgcccgatg
     4621 ccgtccttgt attgctggcg tcttaaaacg tcgagcatac tttttgaggc ttcggcgttt
     4681 atcaattgtc cgagttcgag catttccagt atcccgacca tatctttcgg cgaagacatt
     4741 ccgagtccga aattttttat caccgggtca gcagcgacgc ggctgtcttt ctcgctgccg
     4801 aaaattctgc ggattgattt ggtgtcggta aaacccaggc tttccattcg cgcgttgacc
     4861 gcatccgacg tcactttttc caggaccaga ttggtcgccg tgttgtctga gacgacaatc
     4921 atcaggttaa ccgccgtgcg caaatcgatt ttcgtattgt cggaaaactc gcccaaaatg
     4981 ccggagccgc cgactttttt ttctttggtc agaacgattt cgtccgtcca acggagcttg
     5041 ccttcattaa tctgaaaata agtttcgacc attatcggca gctttatcgt gctcgccgtg
     5101 cggacgcgct cgccgccgcg cagggaaaat gtttctcgcg tatcgaggtt tttcgcgtaa
     5161 atccagactt ttcccttgaa atctctgatt gccgcattga tgcgctcgtc gagcgtcggc
     5221 ggcgcgccgg acggcaaaac cgcattcgac tttaacggcg tcgttttttt ctgagcgaaa
     5281 acgaaaactg aaaacagcag catcaaaacc agacaaaaag aaatttgttt cagcattaat
     5341 tatctccgac cttttcctga taaattgcgg cgggcgcgag cggcgcgcgg taaagaaacc
     5401 ttataatttt atatttcccc gccgcaaata aaccactgta atttatctca gaaaaccctg
     5461 caaaacaaga cgcgattttt tcatccgacg cttttaacgc ttcgcggaaa tgcaaaaggc
     5521 tccgagaagc gttataataa agttgacaaa aatgtaaaga tttataaaat tgaatttttt
     5581 tacagaggtt aaacagaaaa agtagtaatg ccaaagatag cagacataca ggaaacacca
     5641 aatccgaacg cggtgaaatt tattttaaaa gagccggtca gccacggcac ttcgcattct
     5701 ttcaagacgg cggaagatgc cgcggacaac gcgctggcga agtctatttt cgacatcggc
     5761 aatgtcgttt cggttttcta tatggacaag atgataacgg tcgagaaaac cgacgaagcc
     5821 gattgggacg agattctgcc tacattggca gtgccgattc gagcggcgga agccgtcagc
     5881 gtgacaaacg gaaacggcgc ggcaaaatcg gtcggcggcg cgattgcggc ggcgcttgac
     5941 gataatccgc aggttcgcga aatcgaagcg cttctggacg agcgcattcg cccatatctg
     6001 gcgagcgacg gcggctggct ggaaatcgtc gggttggaag acaagacttt acggattcgc
     6061 tatgaaggcg cctgcggcag ctgcccgtcg agcttgaccg gaacgctgat ggcgattgaa
     6121 aatatgatta aagaagaaat cgacccggaa atttccgtta tcacggttta aaacattagc
     6181 aattaataat
//
LOCUS       DASWNE010000778         3148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_484845, whole genome shotgun sequence.
ACCESSION   DASWNE010000778 DASWNE010000000
VERSION     DASWNE010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484845"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="VGB68_15120"
     CDS             <1..975
                     /locus_tag="VGB68_15120"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY0050622.1"
                     /translation="AVSTSSAAPQSMTLATSGATALQAEPLPQNNPIVSGNPKLEAAM
                     RRIEQSRRMYYAEEEKPKPHLTAVPQQLAQPKTFPYAVPNPATESPKPENIPLKAQPK
                     TFIIEKKAENMPEKKAEKFDTNKLPPLSQVVKPASSLESRSISFKTTENKINAAAPVN
                     DFDEEINDVIAYGEVEAFDPIAEEFEEIDDCAPVSLRFNAGLFDLIIGSFLSFILLSP
                     FMLLNGNLFTLQGLLAFLATFAIVMFIYMTVSVGFLGRTLGMRLFSLEIIDIEENDYP
                     TFHQAAVNSCAYLFSLALGGIGFLPLFFNSEKRAAHDLLAGTMVVREE"
     gene            1068..2615
                     /locus_tag="VGB68_15125"
     CDS             1068..2615
                     /locus_tag="VGB68_15125"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HEY0050623.1"
                     /translation="MNEATLEAVRKELEKQLVNRPFGKIFQLARLTFAIDFRLHEGNY
                     LFIGVEPVNPRVYLIKRKLRDLEKQSINPSPFVLFVRKRLSNAVLQKITKIENERILR
                     FEFFAQDEIEGLKKYALIAQLTGRSANIFLLDERDFILDCLRENSGDGQEIASRYQPP
                     KRESKSEPPAAAGALTPQENSPQINADENGLENKDRSPKNKARKFQSNEVFPKDNFAT
                     LSEALDVYYQNSESEKRFQAKAKSAAAKIKQEIGRREKLLGKLNQDLTNHGEAEKWKR
                     YGDLILANLADAARVGDKVLVVDYFDENTPAIEIEIDENSSLTEAAEKFFKRYTKARN
                     AKEEIAGRLKELETQIADYKLRRERLEQAIAEKDEPFLSEIGGESTSGKTQKTRDKES
                     GAIKGVRKFASSDGFEILVGKGSKDNDYLTFRIAKSLDFWLHASDYGGSHVVVRNPNR
                     LENLPPQTLLEAAEIAAFYSQGRNQVKAAVNYTQKKFVNKPKGSAAGLVSLSSFKTIL
                     VEPKISV"
     gene            complement(2705..>3148)
                     /locus_tag="VGB68_15130"
     CDS             complement(2705..>3148)
                     /locus_tag="VGB68_15130"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY0050624.1"
                     /translation="PAVPAAIMQAELKGLDGGKFKLEDYKGKVVLINLWATWCGPCKG
                     EMPHLVELQNTHKDKGFEILGLDTDESDTEAKVKSFAEEMKLNYTLGWVDEKTYNELL
                     KLSKFPGIPQSFLISREGQILSIFTGGSPSTIAKLKDMVGKTVSE"
BASE COUNT          854 a          788 c          772 g          734 t
ORIGIN      
        1 gcagtttcga ccagcagcgc cgcgccgcaa tcgatgacgc tggcaacgag cggcgctacg
       61 gcgctgcaag ccgagccgct cccgcaaaat aatccgattg tttccggcaa tccgaagctg
      121 gaagccgcga tgcgccgcat cgagcaatcg cgccggatgt attacgccga agaggaaaaa
      181 ccgaaaccgc atctgaccgc cgtgccgcag cagttggcgc agccgaaaac ttttccctac
      241 gccgtcccga atccggcgac ggaatcgccg aagccggaaa acattccgct gaaagctcag
      301 ccgaaaactt ttattatcga aaagaaagct gaaaatatgc ctgagaaaaa ggctgaaaag
      361 tttgacacca acaagcttcc gcctttgtcg caggtcgtaa agccggcttc gagcctcgaa
      421 agccgttcga ttagtttcaa aacgactgaa aataaaataa acgccgcagc gccggttaac
      481 gattttgacg aagaaattaa tgacgttatc gcttacggcg aagtcgaagc cttcgacccg
      541 atagcggaag aatttgagga aatcgacgat tgcgcgccgg tttcgctgcg ctttaacgcc
      601 gggcttttcg atttgattat cggctcgttc ctgagcttta ttctgctgtc gccgtttatg
      661 cttttaaacg gcaatttgtt tacgctgcaa ggtttgctgg catttctggc tacttttgca
      721 atcgttatgt ttatttatat gacggtcagc gtcgggtttc tgggcagaac tctgggaatg
      781 cgtcttttct cgctggaaat catcgatatc gaagaaaacg attacccgac gtttcatcag
      841 gcggcggtta attcctgcgc ttatcttttc tcgctcgcgc tcggcggcat cggttttctg
      901 ccgttgtttt tcaacagcga aaagcgcgcc gctcacgacc tgctggcggg aacgatggtc
      961 gtcagagaag aataacgaaa ttcgaaaaat ttaaaaattc cggcgagtag ctttgaaaag
     1021 ttactcgccc tttttattgt gctaatctgt ttatttaata tcggtttatg aacgaagcga
     1081 cacttgaagc cgtcaggaaa gaactggaaa aacaactggt caatcgtccc ttcggcaaga
     1141 ttttccagct cgcgcgcctg acgtttgcga ttgattttcg ccttcacgaa ggaaattatc
     1201 tttttatcgg cgtcgagccg gttaatccgc gcgtttattt aattaaaaga aagctgcgcg
     1261 atttggaaaa gcagtcaatc aacccgtcgc cgtttgttct tttcgtccgc aaaaggcttt
     1321 ccaacgcggt tttgcaaaaa atcacaaaaa ttgaaaacga gcgcattctg cgttttgaat
     1381 ttttcgctca ggacgaaatc gagggcttaa aaaaatacgc gctcatcgca caactgaccg
     1441 gacgctcggc aaatattttt ctccttgacg aacgcgattt tattctcgac tgcctgcgcg
     1501 aaaattccgg cgacggtcag gaaatcgcca gtcgttatca accgccgaaa cgagaatcaa
     1561 agtcagaacc gcctgcggca gcgggcgctt taacgccgca agaaaatagt ccacagataa
     1621 acgcggatga aaacggatta gaaaacaaag accggagccc gaaaaacaaa gcccgaaaat
     1681 ttcaatcaaa cgaagttttc ccgaaagaca atttcgcaac gctttcggaa gcgctcgacg
     1741 tttattatca aaattcggaa agcgaaaaac gatttcaggc gaaagcaaaa tccgccgcgg
     1801 cgaaaatcaa acaggaaatc ggcaggcgcg aaaagctgct cgggaaactc aatcaggatt
     1861 tgacgaatca cggcgaggcg gagaaatgga agcgttacgg cgatttgatt ctggcgaatc
     1921 tggcggacgc cgcgcgcgtc ggcgacaagg ttctggtcgt cgattatttc gacgaaaata
     1981 cgcccgccat cgagattgaa attgacgaaa attcgagcct gaccgaagcc gccgaaaaat
     2041 ttttcaagcg ttatacaaag gcgcgcaacg cgaaagaaga aatcgccgga agattaaagg
     2101 agctggaaac tcagattgcg gattacaaat tgcggagaga gcgactcgaa caggcgattg
     2161 cggaaaagga cgagccgttt ttatcggaaa ttggcggcga aagcacaagc ggcaaaacgc
     2221 agaaaaccag agacaaagaa agcggagcga taaaaggcgt gcgaaaattc gcttcttccg
     2281 acggctttga aatcctcgtc ggcaaagggt cgaaggacaa tgattaccta acttttcgca
     2341 tcgccaaatc gctcgatttc tggctgcacg cgtctgacta cggcggctcg cacgtcgtcg
     2401 tcagaaatcc gaaccggctc gaaaacctgc cgccgcagac gcttctggaa gccgccgaaa
     2461 tcgccgcctt ttacagccag ggcagaaatc aggtgaaagc cgccgttaac tacacgcaga
     2521 aaaaatttgt gaataagccg aaaggctcgg cggctggtct ggtcagtctt tcgagcttta
     2581 aaacgattct ggtcgagccg aaaatcagcg tctaaaagac ggaggcaaaa ataaagcagc
     2641 cgcaagccgg taaaatttat cttccggctt gcggctgctt tattttgtcc tttgccttaa
     2701 agacttactc gctgacggtt ttgccgacca tatctttcag cttcgcaatc gtcgaaggac
     2761 tgccgcccgt gaaaatactc aaaatctgtc cttcgcggct gatgagaaaa ctttgcggaa
     2821 tgccgggaaa cttgctgagt tttaaaagct cgttgtaggt tttttcgtca acccagccga
     2881 gcgtgtaatt gagcttcatt tcctcggcga aacttttcac cttggcttcc gtatcgcttt
     2941 cgtccgtatc caatccgaga atttcgaagc ctttgtcctt gtgcgtgttt tgcagctcga
     3001 ccagatgcgg catttcccct ttacaaggtc cgcaccacgt cgcccagaga tttatcaaaa
     3061 cgactttgcc tttgtaatct tcgagcttaa actttccgcc gtccaaacct tttaactcag
     3121 cctgcattat cgccgccgga acagccgg
//
LOCUS       DASWNE010000779         7891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_61820, whole genome shotgun sequence.
ACCESSION   DASWNE010000779 DASWNE010000000
VERSION     DASWNE010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7891
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61820"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="VGB68_15135"
     CDS             complement(<1..1019)
                     /locus_tag="VGB68_15135"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEY0050625.1"
                     /translation="MNGNRFSPRTIGLLLADAAIIFGAIILALCLRLSVEGTIYQLNE
                     NNGWLKVTLATLVCLLVLYFYDLYDYTVMGNRRELLLRMIQALGIAWALLALLFYFVP
                     PLMIGRGTSVYSVIIVLILLLAWRVLIHLLTGHPEIGEKILIVGAGQAAIDTAQAILK
                     RRDAGYRIIGFITEDYESVKMKLPEARVLGTTANLEEIVHQEKIDRIVIAVRERRGAF
                     PTEMLLRMSLAGDVSIEECTSFYERITGQVHLDMLRPSWLIFAGRGRDTRLKMVFREA
                     VHRTLGLIGLVLSFPIALITAILIKLDSKGPIFYRQERLGKNGRTFQVIKFRSMRTDA
                     EANGKP"
     gene            complement(1131..2144)
                     /locus_tag="VGB68_15140"
     CDS             complement(1131..2144)
                     /locus_tag="VGB68_15140"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEY0050626.1"
                     /translation="MSILRALQKKQSEQSAEQSPQTAVAVSNVVPFEKNATTRRANTP
                     PELFAGQDIKIGSPNPAFIEQNNDSSIGMALPNAGTEFTAGATLNAVSLTRAADAALP
                     DFIRWTVEAERVEPRLVAITSPHSTYCEEYRSLRTHVLHKSQKQKLQSIVVASVSPSE
                     GKSVTALNLSWLLAQTDGVKALLIDSDLRMPSLTDYLGIETDKGLSEILAGKAVLKDS
                     IIKLEPSGLHLLPGGEARSDVAELISGPKFKEILREAKEMFDYVIIDAPPLGIFTDAN
                     VLINHADGALLVVRANKTRYSIVDRVLETIPRERMMGVILNQSEDVLEETHYNYGYYG
                     KKK"
     gene            complement(2253..3326)
                     /locus_tag="VGB68_15145"
     CDS             complement(2253..3326)
                     /locus_tag="VGB68_15145"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0050627.1"
                     /translation="MYKEFYGLEDLPFTLTPDPRFIVFTPSYNEVLASLYYGLENAKG
                     LIVLTGEVGTGKTTALRWILRRLDSSVLAAYVFNPRLSIDEFYHHVTQMLGIKDWNNK
                     AELLSLMGRVLEERHRRGLRTVLIIDEAHELSDYVLEEIRLLMNFESDNAKHLQIVLT
                     GQPELREKLNQPNLRQLKQRVALRCKIHSLPNVDETERYITERLLIAGSDQPNVFTPG
                     AIDFIFQCSEGIPRQINNLCDNSMLAGYAAGEQIIGRAIIEEVADNLDLLPRRENLLA
                     SEQKIENMGAPRLLTDEAREELWNNKSRIEPAKPKMFQAEAKAVNGTLNGASTNGHKN
                     GKYNSYSDLDDEINLEIGEGGFY"
     gene            complement(3433..5094)
                     /locus_tag="VGB68_15150"
     CDS             complement(3433..5094)
                     /locus_tag="VGB68_15150"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEY0050628.1"
                     /translation="MAVEFRQKSMGEIIRMLKRRKWLMLLPTLAAALAVAYVVSKLPS
                     IYESTTLLTLKPPTISEKVVQSLSDEDLSQQLQTINQEVLSRSSLEPMIAKYKLFEME
                     KEGGMPTELIIEKMKNNIRVEPERSDNEKVAAFRITYKDRKPEAARNVAAELASKYVN
                     AQIIASTQSAEVTREFIDNQLGQAKTALDTIEKQRLDIMMQNVETLPESAQGLIAQLE
                     GLRKREETIAKEKETLILERGRLNDSIRALNSQARLIEDYGEKETQEATTKAAQIEDT
                     PAYGQLIQKRAELTARLEKFKLQYREKHPDVVDTQTQIDKVNEELAALAKNTDLRVKQ
                     ANRTSSRKAELQKKNLEIEKQKAESQIGLIEQQFQMKDEELRQNNVLISGLEAKINTI
                     PNVKVALEAVNNQYLSAKTTYDDLLKKKNDASLQVERESNAQGETIRVVDAANLPQSP
                     VNASKRYMAVLMGAAVGLVVGLFLAALFEVPRLFRIQNIEDAKHYTGLPVLASVPPLL
                     TQQEIAWQKRSGWLRLMAGVIAALASIPLLIMALQATRIFEKIVS"
     gene            complement(5139..5306)
                     /locus_tag="VGB68_15155"
     CDS             complement(5139..5306)
                     /locus_tag="VGB68_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050629.1"
                     /translation="MFQGELLNVNLLSADVPESLALLVFGVCLIGATVGLRRLLKRYD
                     EEKTDKSLRQK"
     gene            complement(5311..7695)
                     /locus_tag="VGB68_15160"
     CDS             complement(5311..7695)
                     /locus_tag="VGB68_15160"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0050630.1"
                     /translation="MSFRECQFTKISLLAVLLGLSLLFSSACTGEATKQKHLTRGEEF
                     LQKRKFRQAVMEFRAAADIDKNSAEAHYGLARAHENLGEVYEAIKELQEVGQLAPANL
                     DAKARLGNYFLLIAPPQTDEAQKMIDEIFAVNPNFVEGHILKASLLSAQNRPETQILE
                     VLNTAIEIEPRRVETYISLARFFMKQEKAPEAEQTIQKAIAVGDKSPVGYIEYGRFYD
                     FAGRPADAEAQFKKAVELAPQNYEARQASASFYLAQRQLEKAEQAYKDLAQILENSAE
                     GLTELAEFYVTVEREEDAIKTFQAILNDAPEHAPARYRLAEIYLERREMAKVSEQVEK
                     LLAINNTDAEALMLRARVKLQENSTEEAIKDLEEVLKRQPSFKSALFYMAQARLALGQ
                     VDQARAFIGDLEKYHPNYLSSKILKIQASFTANEPQKALQEANDLIEIVQTAYPTAET
                     SAQQLQQLRVRAISSRGLAYIELGKLAEARADLEEIVRLSPNSASAFVNLAKVSAAQK
                     NLAEALGFYEKALAIDKQNFDALSGAVGVLTSQKQFAAAHEKLDRAIAENAGDKKVLP
                     AFHYLKADVARSENNLPAAESELAKAIELDDTYLPAYSAYAALLISQNQIERAIEQYR
                     RVVEKKQSASVYTLIGMLEDARGNYDEAEKNYRKALEIAPETPMAANNLAWNIADRES
                     GNLDEALTLAQSCVDKNSASAGFYDTLGWVYFKKGLMSPAVEQLKKAVALEDAGAARS
                     GRAANPAYRLRLGQALASAGDKPSARREVELALEQEKDLTQKEVQDAKSLLSSL"
BASE COUNT         1647 a         2203 c         1984 g         2057 t
ORIGIN      
        1 ggtttgccgt tcgcctcggc gtcggtgcgc atcgagcgaa acttgatgac ttgaaacgtt
       61 ctgccgtttt ttcctaatcg ctcctgccgg taaaaaatcg gacctttcga atcgagcttg
      121 attaaaatcg ccgtaatcaa ggcaatcgga aacgataaaa ccaatcctat caatcccaga
      181 gtgcgatgaa ccgcctcgcg gaaaaccatc ttcaggcgcg tgtcgcgtcc gcgtccggca
      241 aaaatcagcc acgaaggtcg cagcatatcc agatgaacct gcccggtgat tctttcataa
      301 aacgaagtgc attcttcgat ggagacgtcg cccgccaggc tcatccgcaa aagcatctcg
      361 gtcggaaaag cgccgcgccg ctcgcgcacg gcaatcacga tgcggtcgat tttttcctga
      421 tgaacgattt cttccaaatt cgccgttgtg ccgagaacgc gcgcttccgg caatttcatt
      481 ttcaccgatt cgtaatcttc ggtgataaag ccgataatgc gatagccggc gtcgcggcgt
      541 ttcaaaatcg cctgagccgt gtcaatggcg gcttgtccgg cgccgacaat caaaatcttt
      601 tcgccgattt ccgggtgtcc ggtcaaaagg tgaatcaaca cgcgccacgc cagcagcaaa
      661 atcaggacga taatgaccga atagacggaa gttccgcgcc cgatcatcaa aggcggcacg
      721 aaatagaaaa gcagggcgag cagcgcccac gcgatgccga gcgcctgaat catccgcaaa
      781 agcagctcgc ggcgattgcc catcacggtg taatcgtaaa ggtcgtaaaa ataaagaacc
      841 agcagacaaa ccagcgtcgc cagggtcact ttcagccagc cgttgttctc gttcagctgg
      901 taaatagtcc cttcaacgct gagccgcaaa cacaaagcga ggattatcgc gccgaagatt
      961 atcgcggcat cggcaagaag aagtccaatc gtgcgagggc tgaagcggtt tccgttcatt
     1021 gttttgtggc gacgcggcga tgcggtgact aagcgacacg gagaaattga ttcaagcttt
     1081 cagcatttcg attttttcgc cgcgtcgccg cgtctctcat tgtccgcgtc ttattttttc
     1141 ttcccgtaat agccgtaatt gtaatgcgtc tcttctaaaa catcttccga ttgattcaaa
     1201 atcacgccca tcatacgctc gcgcggaatc gtctccagca cgcggtcgac gatgctgtag
     1261 cgcgtcttgt tggcgcgcac gacgagcagc gcgccgtcgg cgtgattaat caaaacgttg
     1321 gcgtccgtaa aaattccgag cggcggcgcg tcgatgatga cgtaatcgaa catttctttg
     1381 gcttcgcgca gaatttcttt aaacttcggt cccgaaatca gttccgccac atccgagcgc
     1441 gcttcgccgc ccggcagaag atgcaagccg gaaggctcta atttaataat cgaatctttc
     1501 agaacggctt ttcccgccag gatttccgac aagcctttat cggtttcgat gccgagataa
     1561 tcggtcaggc tcggcatccg caaatcgctg tcgataagca gcgctttgac gccgtcggtc
     1621 tgcgccagca gccacgataa attaagcgcc gtgaccgatt tcccttcgga cgggctgacg
     1681 ctggcgacga cgatcgactg caatttctgc ttctggcttt tgtgcagaac gtgcgtgcgc
     1741 aggctgcggt attcttcgca gtaggtcgaa tgcggcgacg tgatggcgac aagtcttggc
     1801 tcgacgcgct cggcttcgac cgtccacctg ataaaatcag gcagagcggc gtcggcggcg
     1861 cgcgtcaagc tgacagcgtt caaagttgcc ccagcagtaa attccgttcc cgcattaggg
     1921 agtgccatcc caattgaaga atcgttgttc tgctcaatga acgctggatt cggcgaaccg
     1981 atttttatat cctgtccggc aaacaattcc ggcggcgtat tggcgcggcg cgtcgtcgcg
     2041 tttttttcaa acggcacgac attgctgacc gccacagccg tctgcggcga ctgctcggcg
     2101 gattgttcgg attgtttttt ctgtaatgct ctaaggatgc tcattcgatt actccgtaat
     2161 catttctgat tcaagattcc gaattccgta tttaaggctg cggattgaat gctgcgttct
     2221 caatcccgaa tcttaaatcc cgaattttga atttaataaa acccgccttc tccgatttcg
     2281 agattgattt catcgtccaa gtcgctgtac gagttgtatt taccgttttt atgcccgttg
     2341 gtcgatgctc cgttcaaggt tccgttgacg gctttcgctt ccgcttgaaa cattttcggt
     2401 tttgccggct cgatgcgcga tttgttattc caaagctctt cgcgcgcctc gtcggtcaga
     2461 aggcgaggcg cgcccatatt ttcaatcttt tgctcgctcg ccagcagatt ttcgcggcgc
     2521 ggcagcaaat ccagattgtc ggcgacttct tcgataatgg cgcgcccgat gatttgttct
     2581 ccggcggcgt aaccggcaag catcgagttg tcgcacagat tgttaatctg gcgcggaatg
     2641 ccttccgagc attgaaagat aaaatcgatc gctccgggcg taaaaacgtt cggctgatca
     2701 gagccggcaa tcaaaagacg ctcggtaata taacgctcgg tctcgtcgac gttcggcagc
     2761 gaatgaatct tgcagcgcag cgccacgcgc tgtttcagct ggcgcaggtt aggctggttc
     2821 agcttttcgc gcagctcggg ctgcccggtc agaacgattt gcagatgctt ggcgttgtcc
     2881 gattcgaaat tcatcaaaag gcgaatttct tccagcacgt aatcggacag ctcgtgcgct
     2941 tcgtcgataa tcaaaaccgt gcgaagcccg cgccggtgac gctcttccag aactctgccc
     3001 atcagcgaga gcagctcggc tttattgttc cagtccttga tgccgagcat ctgcgtgacg
     3061 tgatgataaa attcgtcaat cgacagacgc ggattgaaga cgtaagccgc caaaacggaa
     3121 gaatccagac ggcgcaaaat ccatctgagc gcggtcgttt ttccggttcc tacttcgccg
     3181 gtcaggacaa ttaagccttt ggcgttttcc agaccgtaat acaggctcgc caaaacctcg
     3241 ttatagctcg gcgtaaacac tataaaacgc gggtccggcg tcagcgtgaa aggtaaatct
     3301 tctaaaccgt aaaattcttt atacattttt cgcctctttt ccaaaggatt caggatttcg
     3361 gattcaggat tcaggattcc gaagaagttt tcaatcttga atcctgaatc ctgaattttg
     3421 aatcccgaat atctaagaaa caatcttttc aaaaattcgg gtcgcctgca gcgccataat
     3481 caaaagcgga atgctcgcca gcgccgctat cacgccagcc attaatctga gccagccgga
     3541 gcgcttctgc caggcgattt cctgctgcgt aagcagcggc ggcacggacg ccagcacggg
     3601 caagccggtg taatgcttgg cgtcctctat gttttgaatt ctgaacaggc gcggaacttc
     3661 aaacagcgcc gctaaaaaca aaccgacgac cagcccgacc gccgcgccca tcaaaaccgc
     3721 catatagcgt ttcgaggcgt tgacgggcga ctgcggcagg tttgccgcat cgaccacgcg
     3781 aatcgtttcg ccctgcgcgt tcgattcgcg ctcgacctga aggctggcgt cgtttttctt
     3841 tttcaaaagg tcgtcgtaag tcgttttcgc cgaaagatac tgattattaa cggcttcgag
     3901 cgcgaccttg acgttcggaa tcgtgttgat tttcgcttcg agcccggaaa tcagcacgtt
     3961 gttctggcgc agttcttcgt ctttcatctg aaactgctgt tcgatgagcc cgatttggct
     4021 ttcggctttc tgcttttcga tttccagatt tttcttttgc agctcggctt tgcgcgagct
     4081 ggtccggttt gcctgcttaa cgcgcaaatc ggtgtttttc gccagcgccg ccagctcttc
     4141 gttgacttta tcaatctgcg tctgcgtatc gaccacgtcc ggatgctttt cgcggtactg
     4201 caatttgaat ttttccagcc tcgccgtcaa ttcggcgcgt ttttgaatta attgtccgta
     4261 tgccggagtg tcttcgattt gcgccgcctt ggtggtcgcc tcctgcgttt ccttttcgcc
     4321 gtaatcttcg atgagccgcg cctggctgtt caaagcgcga atgctgtcgt tcaggcgtcc
     4381 tctttcaaga atcaaagttt ccttttcctt ggcgatggtt tcctcgcgct tgcgcaagcc
     4441 ctcgagctgc gcaatcagcc cctgcgccga ttcgggcaga gtctcgacgt tctgcatcat
     4501 tatatcgagc cgctgttttt cgattgtgtc cagcgcggtt ttcgcctgac ccagttgatt
     4561 gtcgataaac tcgcgcgtga cttccgcgct ctgcgtcgaa gcgataatct gcgcgttgac
     4621 gtatttgctg gcaagctccg ccgcgacgtt gcgcgccgct tccggcttgc ggtctttata
     4681 ggtgatgcga aaggcggcga ccttttcgtt gtcgcttctt tccggctcga ccctgatatt
     4741 gtttttcatc ttttcgataa tcagctcggt cggcatcccg ccttcttttt ccatctcgaa
     4801 aagcttgtat ttcgcaatca tcggctccag cgaagaacgc gacaaaactt cctgattaat
     4861 cgtctgcagc tgctgcgaca aatcctcgtc cgaaagcgac tggacgactt tttccgaaat
     4921 cgtcggcggc tttaaggtta aaagcgtggt cgattcgtaa atgctcggaa gctttgagac
     4981 gacatacgcg accgccagag cggcggcgag cgtcggcaaa agcatcagcc atttgcgccg
     5041 cttcagcatc cggataattt cgcccatcga cttttgtctg aattcaaccg ccatttcctt
     5101 aatcctcttt tcgcctttaa ctttttactt ttgccttttt acttctgcct taacgattta
     5161 tcggtctttt cttcgtcgta gcgtttcagc aggcggcgca agccgactgt cgcgccaatc
     5221 aggcacacgc cgaaaaccag cagcgccagc gattccggca catccgccga aagcaaattt
     5281 acgttgagca attccccttg aaacatattt ttataaactg ctcagaaggc ttttcgcgtc
     5341 ctgaacttct ttctgagtta agtctttttc ctgttcgagc gccagctcca cttccctgcg
     5401 cgccgaaggt ttgtcgccgg cggaagccag cgcctgtccc aaacgcagcc ggtacgccgg
     5461 atttgccgct ctgccgctgc gcgccgcgcc cgcgtcttcg agagcgacgg cttttttcaa
     5521 ctgctcgacg gcgggcgaca tcaagccttt tttgaaataa acccagccga gcgtgtcgta
     5581 aaatccggcg ctggctgaat ttttgtccac gcacgattgc gccagcgtca gcgcctcgtc
     5641 cagatttccg ctttcgcggt cggcgatatt ccacgccaga ttgtttgccg ccatcggcgt
     5701 ctcgggcgcg atttccaaag ctttgcgata atttttttcg gcttcgtcgt aatttccgcg
     5761 cgcgtcttcg agcattccga tcagcgtgta aaccgatgcc gactgctttt tctcgacgac
     5821 cctgcggtat tgctcgatgg cgcgctcaat ttgattctgc gaaataagca gcgccgcgta
     5881 agccgaataa gcgggcagat acgtatcatc gagttcgatg gctttggcaa gttcggattc
     5941 agccgccggc agattatttt ccgagcgcgc gacgtccgcc ttcagataat gaaaagcggg
     6001 cagaactttt ttgtcgccgg cgttttcggc aatcgctcta tcgagttttt cgtgcgccgc
     6061 cgcaaactgt ttctggctcg tcaggacgcc gaccgcgccg cttaaggcat cgaaattctg
     6121 tttatcaatc gccagcgctt tttcataaaa accgagcgct tcggcgagat ttttctgcgc
     6181 cgccgaaacc ttcgccagat ttacaaaagc gcttgccgaa ttcggcgaaa gcctgacgat
     6241 ttcttccaga tcggcgcgcg cttccgccag ctttccgagc tcgatataag ccaagccgcg
     6301 agatgaaatc gcgcggacgc gaagctgctg caattgctgc gccgaggttt ccgccgtcgg
     6361 gtaagccgtc tggacaatct caatcaaatc gttggcttcc tgcaaagctt tctgcggctc
     6421 gtttgccgta aagctcgcct gaattttcag aatcttgctg gacagataat tcggatgata
     6481 tttttccaaa tcgccgataa aggcgcgcgc ctgatcgacc tgtccgagcg cgagccgcgc
     6541 ctgcgccata taaaacaagg cggatttaaa gctcggctgg cgttttaaaa cttcttccaa
     6601 atcctttatc gcctcttcgg tcgagttttc ctgcaatttg acgcgggcgc gcagcatcag
     6661 cgcttcggcg tcggtgttgt tgattgccag aagcttttcg acctgctcgc tgactttcgc
     6721 catttccctg cgttccagat aaatttcggc gaggcggtaa cgcgccgggg cgtgttcggg
     6781 cgcgtcgttt aaaatcgcct gaaacgtctt tatagcgtct tcttcgcgtt cgaccgtcac
     6841 gtaaaattcg gctagctccg tcagcccttc cgcggaattt tccagaatct gcgctaaatc
     6901 tttataagcc tgctcggctt tttccaactg ccgctgcgcc agataaaacg acgccgacgc
     6961 ctgacgcgcc tcgtaatttt gcggcgccag ctcgaccgct ttcttaaact gcgcttcggc
     7021 atccgccggg cgaccggcaa aatcgtaaaa acgcccgtat tcgatatagc cgaccggcga
     7081 tttatcgccc accgcaatcg ctttttgaat tgtctgctcg gcttcgggcg ctttctcctg
     7141 cttcataaaa aagcgggcga ggctgatgta cgtctcgacg cggcgcggct cgatttcgat
     7201 tgccgtgttc aacacttcga gaatttgcgt ttccggtcgg ttctgcgccg acagcaggct
     7261 ggctttcagg atgtgacctt cgacaaaatt cgggttgacg gcaaaaatct cgtcaatcat
     7321 tttttgcgct tcgtcggttt gcggcggcgc aatcagcaga aaataattgc cgagccgcgc
     7381 tttggcgtcc agattcgccg gcgccagctg cccgacttcc tgcaattcct ttatcgcttc
     7441 gtaaacttcg ccgagatttt cgtgcgctct cgccaagccg taatgcgctt cagccgaatt
     7501 tttatcgata tcggcggcgg cgcgaaattc catcacggct tggcggaatt tgcgtttctg
     7561 caaaaattcc tcgccgcgcg tcagatgttt ttgtttggtg gcttcgcccg tgcaggcgga
     7621 agaaaaaaga aggctcagac cgagcaggac ggcaagcaga ctgattttag taaattggca
     7681 ctctctaaaa ctcatttttt ttaagacgac tttccatagg tcaaacgcgg ctgaaatttt
     7741 ggtttttgca atcgggcaat gactgcaaaa acctttcagc gaattttagc gggcgcttcg
     7801 gggcacgaaa acgaccattt tttcgacctg gaatttattt cagtttttca atacagcaga
     7861 ccgttttctc taacagtctc aatgattacc g
//
LOCUS       DASWNE010000780         1901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_274408, whole genome shotgun sequence.
ACCESSION   DASWNE010000780 DASWNE010000000
VERSION     DASWNE010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274408"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VGB68_15165"
     CDS             complement(<1..187)
                     /locus_tag="VGB68_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050631.1"
                     /translation="MDKLVIEYKHGIAESVDRAIIRAVGKDPKNTGCMAVEPFARDLV
                     FEFRTEEAALSAKARVEM"
     gene            complement(275..1498)
                     /locus_tag="VGB68_15170"
     CDS             complement(275..1498)
                     /locus_tag="VGB68_15170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050632.1"
                     /translation="MFNNETNLNQRQSALGEYAGIPREMQNAPDTAIIQPDNADRKKS
                     RFVVNVGDSITPFEKYADSTRAFGGVVLEKYLTLRHTGGMLYRCQITELKQFMKDENP
                     MMLAPDDRKTASTGYVVKNGQFNGATGMWEDKTVSGSVYLPFYPGDEIVDMTRAKQSL
                     ETGGTVEVTALAGKTAKERDAFQQFYFPNWNRLQAGLDQFPRTLRELRARIEERLKIA
                     VAEVDADLVSVGRDLLKSCTEYRNWALRYIKVQTQIVQAAKLQAGALVTGYNDMAEML
                     MEILEFKREDAILSELNNIAAANYGQVQRIEAQAPSAEADARIAAILENQNRLIEALF
                     NKLEGNETPAPTQASAPSPADEQAIREFEEFQAWRASRSADASNEVEKPAPAPANSRG
                     RGNRPAEKPEMSEVK"
     gene            complement(1532..>1901)
                     /locus_tag="VGB68_15175"
     CDS             complement(1532..>1901)
                     /locus_tag="VGB68_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050633.1"
                     /translation="AEGFSCYGSYRPPAEQDLIALGNIKRDKEKQTAAQSQSPYEALS
                     EETKKRLAASTKNYYADQYKRMQRSAQIYVDNAVEFTKPLMDAMKVNLSEREIKEIVR
                     SATNQEIDCAAEEMRRRLDR"
BASE COUNT          352 a          515 c          499 g          535 t
ORIGIN      
        1 acatttcaac tcgtgctttg gcggataagg ctgcttcctc agttcgaaat tcaaagacta
       61 aatcacgagc aaaaggctcg acagccatac agcccgtatt cttcgggtct tttccgactg
      121 ctctgattat tgccctgtca accgattcag ctattccgtg tttatattca ataactaatt
      181 tatccatatt cgcttttcct tttcttgtat tagtttttat tgcgccgtta ttattccgcg
      241 acggcgcgag cgcgctaccc cttacgggaa atctttattt aacttcggac atttcgggct
      301 tttcagccgg acgattcccg cgccctctgc tattcgctgg tgccggtgcc ggtttttcaa
      361 cctcgtttga ggcatcggcg ctgcgggaag cgcgccacgc ctgaaattcc tcgaattccc
      421 tgattgcctg ttcgtcggca ggcgatggcg cagatgcctg agtcggcgcg ggtgtttcat
      481 tgccttcgag cttattgaac aaggcttcaa tcaaacgatt ctgattttcg agaatggcgg
      541 caatgcgcgc gtcggcttcg gcgctcggcg cttgagcttc aatgcgctga acctgaccgt
      601 aattggcggc ggcgatgttg ttgagttccg acaaaatcgc gtcctcgcgt ttgaattcca
      661 gaatttccat caacatctca gccatatcgt tatagccggt gacgagcgcg ccggcttgca
      721 atttcgccgc ctggacgatt tgcgtctgca ctttgatata acgcagcgcc caatttcgat
      781 attctgtgca ggatttcaaa aggtcgcgcc cgacgctgac taaatcggcg tcgacttcgg
      841 cgacggcaat tttcaatctt tcttcgatgc gagcgcgcag ctcgcgcaat gtgcgcggga
      901 actggtcaag cccggcttgc aggcggttcc agttcggaaa ataaaactgc tggaaagcgt
      961 cgcgctcttt cgccgttttt ccggcgagcg cggtgacttc gaccgttccg cccgtttcca
     1021 gagactgctt ggcgcgcgtc atatcgacga tttcatcgcc cggataaaac ggcaggtaaa
     1081 ccgaacccga aacggttttg tcttcccaca tcccggtcgc gccgttgaac tgcccgtttt
     1141 tgaccacgta gccggtcgat gccgttttgc ggtcgtccgg cgcgagcatc atcgggtttt
     1201 cgtccttcat aaactgcttg agttccgtta tctggcaacg gtaaagcatc ccgcccgtat
     1261 gccgcagcgt gagatatttt tccagcacga cgccgccgaa agcccttgta gagtcggcgt
     1321 atttttcgaa cggcgtaatc gagtcgccga cgttgacgac gaaacgcgat tttttgcggt
     1381 cggcgttgtc gggctggatg attgccgtgt ccggcgcgtt ttgcatctcg cgcgggattc
     1441 cggcgtattc gccgagcgcg gactgtcttt gattcaggtt ggtttcgtta ttaaacattt
     1501 cggtttctcc taaaaaaata ttgcttggtg tctatctatc gaggcggcgg cgcatttctt
     1561 cggcggcgca gtcgatttcc tgattcgtcg cgctacggac gatttctttt atttctcgct
     1621 cgctcagatt aactttcatc gcgtccatca gcggcttcgt aaattcgacg gcgttatcga
     1681 cgtagatttg agccgaacgc tgcatacgtt tgtactggtc ggcgtaataa tttttcgtcg
     1741 aagcggcaag gcgctttttc gtttcttccg acaaagcctc gtaaggcgat tgcgactgcg
     1801 cggcggtctg cttttcctta tcgcgcttga tattgccgag agcgattaaa tcctgttcgg
     1861 cgggcgggcg gtatgagccg tagcacgaaa agccttccgc t
//
LOCUS       DASWNE010000781         2446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_695657, whole genome shotgun sequence.
ACCESSION   DASWNE010000781 DASWNE010000000
VERSION     DASWNE010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695657"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..1836)
                     /locus_tag="VGB68_15180"
     CDS             complement(37..1836)
                     /locus_tag="VGB68_15180"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HEY0050634.1"
                     /translation="MTKTLFSLLFSAAFLTLNVFAQSESMNIPTEWQTFAEQTDYRKT
                     PRYSETIEYSQKLDKSSDLIIYKSFGKSGEGRDLPLLIAADEKTFTPEAARKKGKAVV
                     LIQACIHAGESDGKDAGLALLRDIAITKTRLSLLESVVILYVPIYNVDGHELFSPYNR
                     INQVGPDEMGFRGTSANLNLNRDYMKADAPETRAFLKLWNDWKPDFFIDSHVTDGADF
                     RYNITYEFAHFEEVSPFIKSWMNEIFEKKIVPKVEKEGNLLTHYLEFNGREVSSGVTT
                     FIAAPRFATGYAALRNRGGLLIESHSLKPYKSRVRGTYDVFWKTLEEINLNKESLFEA
                     NRRADAETIRRGQAYDANNKFPLRFEFTKNSVPFDFKGVEYKMEDSEVSGAKRIVYGT
                     KPLDVTIPKFDEVKISAAVAPPLYYIIPPQWQSVIEVLQTHGIRLQRISKPLKIEIES
                     YRLTEPRWSPASFESRLPMSYKTNSLKEMREFPANSVVAALNQETGNVLVHLFEPQSG
                     DSFAAWGFFNAIFEQKEYFSDYIMEPIAREMLSKDENLRREFEERLKDENFRKNANAR
                     LRFFYERSPYFDNRIGLYPVGRIVTKLDAGIFK"
     gene            2092..>2446
                     /locus_tag="VGB68_15185"
     CDS             2092..>2446
                     /locus_tag="VGB68_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050635.1"
                     /translation="MISKVWIRKALSLCLVVAIYATYSMVGLASPDKLMGELTVSGKA
                     VNGEIPVVKVNGEAAQSGRAIFSSSTITTSESSSAVINLGKIGKIELAPNTTISVSFS
                     ENGLNGDLAAGKVTAL"
BASE COUNT          617 a          586 c          560 g          683 t
ORIGIN      
        1 ttcttttctt tagccatttg cctttgacct tttgccttat ttaaaaattc ccgcatccag
       61 tttcgtcacg attctaccga ccggataaag cccgatgcga ttgtcgaaat acggcgagcg
      121 ctcatagaaa aaccttaagc gggcgttcgc gtttttacga aaattttcgt cctttagtct
      181 ttcttcaaat tcccttcgca aattttcgtc tttcgacagc atctcgcgcg cgatcggctc
      241 cattatgtaa tcgctgaaat attctttctg ctcgaaaatc gcgttgaaaa atccccaggc
      301 agcgaacgaa tcgcccgatt gcggctcaaa cagatgaacc agaacgtttc ccgtttcctg
      361 attcagcgcg gcgaccacgg agtttgccgg aaactcgcgc atctctttga gcgaattggt
      421 tttataactc atcggcagcc ggctttcaaa cgaggcgggc gaccatcgcg gctcggtcag
      481 gcgatagctt tcaatctcga ttttcagagg cttcgagatt ctctgaagac ggatgccgtg
      541 cgtctgtaaa acttcgatga cgctttgcca ttgcggcgga ataatgtaat aaagcggcgg
      601 cgcgacggcg gcggaaattt ttacttcgtc gaatttcgga atcgttacgt ccagcggttt
      661 tgtgccgtaa acgatgcgtt tcgcgccgga aacttcgctg tcttccatct tgtattcgac
      721 gcctttaaaa tcgaacggaa cggaattttt tgtaaactcg aaacgaagcg gaaatttatt
      781 attcgcatcg taggcttgcc ctcgccgaat cgtctcggcg tcggctcggc gatttgcttc
      841 aaataagctt tctttattca gatttatttc ttccagcgtt ttccagaaaa cgtcgtaagt
      901 tccgcgcaca cgcgacttat acggcttcaa ggaatgcgat tcaatcaaga gtccgccgcg
      961 gtttcttaag gcggcgtagc cggtcgcaaa acgcggcgcg gcgataaaag tcgtcacgcc
     1021 ggagctcacc tcgcgcccgt taaattccag ataatgcgtc aaaagattgc cttctttttc
     1081 cactttcggc acgatttttt tctcgaaaat ctcgttcatc cagcttttga taaacggcga
     1141 gacttcttcg aaatgcgcga actcatacgt gatgttgtag cgaaaatccg cgccgtccgt
     1201 tacgtgcgag tcgataaaaa aatccggctt ccaatcgttc cagagcttta aaaaggcgcg
     1261 cgtctccggc gcgtccgcct tcatataatc gcggttcaga ttcaggttcg ccgaagtccc
     1321 gcgaaagccc atctcgtcgg gtccgacctg attgatgcgg ttatacgggg aaaaaagctc
     1381 gtgaccgtca acgttgtaaa tgggaacgta gagaatcacg accgattcga gcaggctcag
     1441 acgagttttc gttatcgcaa tatcgcgcag cagcgccaag cccgcatctt tgccgtccga
     1501 ttcgcccgcg tgaatgcagg cttgtatcaa aacgacggct tttccctttt ttcgcgccgc
     1561 ctcgggcgta aacgtcttct cgtccgccgc aatcagaagc ggcaaatcgc gaccttcgcc
     1621 gcttttgccg aaagatttgt aaattatcaa atcggaagac ttatctaatt tttggctgta
     1681 ttctatcgtt tcggaataac gcggcgtttt ccggtaatcg gtctgttcgg caaaagtttg
     1741 ccattcggtc ggaatattca tcgattcact ctgagcaaag acgtttaaag tcagaaaagc
     1801 ggctgaaaac agcagcgaaa aaagcgtttt agtcatattc tcgtttaacg aaacaatttt
     1861 ctacgataat aaaccatttt tcatacaaaa agaactttct tatagggcaa aaatctgtta
     1921 agataatttt catcaaattt atttgttctg agttcgtaag tcgtataaaa acttagtctt
     1981 aaatttagca attgtcaccg ttttttattg acatcggcaa ataattcgtg taaaagatac
     2041 aaggcataga ttttttaaat tattgattca aagcggttca agaggagaaa tatgattagc
     2101 aaagtttgga ttcggaaagc actttccctg tgcttggttg tcgctatcta tgcgacatac
     2161 tcaatggtcg ggttggcaag tcctgacaaa ttgatgggcg aactgacggt ctcgggcaaa
     2221 gccgtcaatg gtgaaattcc ggttgttaaa gttaacggtg aggcggcaca gagcggtcgc
     2281 gcaatctttt cgtcaagcac gattacaaca tctgaaagtt ccagcgcggt tatcaacctg
     2341 ggcaaaatcg gcaaaattga acttgccccg aacacgacca tttcggtgtc tttcagtgaa
     2401 aacggtttaa atggcgacct tgctgccggt aaagtaacgg ctttaa
//
LOCUS       DASWNE010000782         2688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_26540, whole genome shotgun sequence.
ACCESSION   DASWNE010000782 DASWNE010000000
VERSION     DASWNE010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26540"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..717
                     /locus_tag="VGB68_15190"
     CDS             199..717
                     /locus_tag="VGB68_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050636.1"
                     /translation="MKSEHRCPAKLALFLLLIFASGFIVKAQDGRSVVLTGRELTAVT
                     PTGFYFEGQSAPTQMRNAAAVRFGGNRHALAGLVDTSGYSTDITAKYQGFFITDSRIR
                     IGGRELPTGAYGFGFRGERFNVFDVGGNPVVEVDARSDRALRRPRPLMMTKNGNEIRL
                     YAGRSYVVITAN"
     gene            950..>2688
                     /locus_tag="VGB68_15195"
     CDS             950..>2688
                     /locus_tag="VGB68_15195"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0050637.1"
                     /translation="MNRLFFNRSRMRKPFSIACALFLLVSAFSFGIPAQNILPPTNKQ
                     PVDRTRLEVIEELSESLANDLLELSVATRDRDLELTAEFFPKQLAAKAFPSRPLPAKA
                     QVKWVGTHGWESGNKTDSAIALATMPSAEFLRNWSEFLAHFSEIEDARFKVKEANFDE
                     KAQAVLGAEQPTAVVGASGKARIAFYVIGRDTEGRREWARGTFMTNVRAAANKHWQFD
                     SFDLISFDSMVAGVDLFSEVAVPAGVGVNIPSFGTPENSGFIWHGAAAADFNADGWID
                     LFVTAAARNYLYLNDGKGSFRDASAEAGVKTLASGTAPLVFDYDNDGDPDVFISAVGQ
                     QILLQNTFKQNGKLEFSDVSLESGVAKNAIGFSAAAGDVNGDGRLDVYVTSYNRYGQV
                     TPDSWFRATNGTPNLLFVSQPDGTFREEAAKWGVDDRRWSYAAEFADVNGDGKIDLYV
                     ANDVGEKAMFINRGDKFVDEAMERGVLDPGNGMGVSFGDYNNDGRLDIHASNMSSTAG
                     NRILTRLFPNSSAKDNVLKKLAAGNNLFENTGDGKFKDVTAEVGGFSGGWAWGGGFID
                     FDNDGWEDIYTPNG"
BASE COUNT          626 a          695 c          755 g          612 t
ORIGIN      
        1 gagataaaaa acaagctctg aaatataatg aaatccggtg cggttttccg acggaaaatt
       61 tatttagccg gctgccgttt tgtaaattcg ccgccgcttc atttgcaaat aatcgcatcg
      121 ccgcttttcc gcacgcgccg gtttctgtag tatattggca ttcatttcag acaaaaaata
      181 aatcaaacgg gagaatttat gaaatcagag catcgctgtc cggcaaaatt agctttattt
      241 ttgctgttga ttttcgcgtc cggctttatc gttaaggcac aggacggcag aagcgtggtg
      301 ttgaccggca gggaattgac cgccgtgacg ccgaccggct tttattttga agggcaatcc
      361 gcgccgacgc agatgcgcaa tgcggctgcc gtcagattcg gcggaaaccg ccacgcgctc
      421 gccggtctgg tcgatacttc gggttactcg acggatatta ccgctaaata tcagggattt
      481 tttattaccg attcgcgaat cagaatcggc ggcagagagc tcccgaccgg agcttacggc
      541 ttcggcttcc gcggcgagag gtttaatgtt ttcgacgtcg gcggcaatcc ggtcgtcgaa
      601 gtcgacgcgc gcagcgacag ggctctgcgc cgaccgcgcc cgttgatgat gacgaaaaac
      661 ggcaatgaaa tcaggcttta cgccggacgc tcttacgtcg ttataacagc caattgaaag
      721 tttaagaata aaccgtggcg cgaaaataaa aacgctgctt tttccggcgg cagcggcggc
      781 tgaaataaaa gaagcggttt ccggcgcatt ttctttgcaa accgtttatc aaatagctaa
      841 gattagcact gcgtttgttc tctaaagcac aatacaacca aataccgcgt tactttacgg
      901 gcagagcctc gctcggcttc cgttatattc cgttatataa ggtaaataaa tgaatcgtct
      961 atttttcaat cgttccagga tgcgaaagcc tttttccatc gcctgcgcgc tgtttttgct
     1021 ggtttccgca ttttcgtttg gaattccggc gcaaaatatc ttgccgccga cgaataaaca
     1081 gccggttgac cgcacgcgtc tggaagttat cgaagagctt tccgagtcgc tggcgaacga
     1141 cctgctggag ctttcggtgg cgacgcgcga ccgcgatttg gaactgacgg cggaattttt
     1201 cccgaaacaa ctggcggcaa aagcatttcc gtcacgccct ctgcccgcca aagctcaggt
     1261 caaatgggtc ggcacgcacg gctgggaaag cgggaataaa accgattcgg cgattgcctt
     1321 ggcgaccatg ccgagcgccg aattcctgcg aaactggtcg gaatttttag cgcatttttc
     1381 cgaaatcgaa gacgcgcgct ttaaagtcaa ggaagcgaat tttgacgaaa aagcgcaggc
     1441 tgttctcggc gcggagcagc cgacggcggt cgtcggggcg agcggcaaag cgcgcatcgc
     1501 gttttacgtc atcgggcgcg atacggaagg caggcgcgaa tgggcgcgcg gcacatttat
     1561 gaccaatgtt cgcgccgcgg caaataaaca ctggcagttt gattcgttcg acttaatcag
     1621 tttcgattcg atggttgcgg gcgtcgattt attttcggaa gtcgccgttc cggctggcgt
     1681 cggcgtaaat attccttcgt tcggaacgcc cgaaaacagc ggtttcatct ggcacggcgc
     1741 ggcggcggcg gattttaacg ccgacggctg gattgacctt ttcgtgaccg ccgccgctcg
     1801 caattatctt tatttaaacg acggcaaagg ctcttttcgc gacgcttccg ccgaagcggg
     1861 cgtgaaaact ttagccagcg gaaccgcgcc gctcgtcttt gattacgaca acgacggcga
     1921 cccggacgtt ttcatttcgg cggtcggtca gcagattctt ttgcaaaaca ctttcaaaca
     1981 aaacgggaag ctcgaattca gcgacgtttc gctcgaatcg ggcgtggcga aaaacgctat
     2041 cggcttcagc gccgcggcgg gcgacgtcaa cggcgacggg cggctcgacg tttacgttac
     2101 ttcttataat cgctacgggc aggtgacgcc ggattcgtgg tttcgcgcga ccaacggcac
     2161 gccgaatctg cttttcgtca gccagccgga cggcactttc cgcgaggaag ccgccaaatg
     2221 gggcgtcgac gaccggcgct ggagctacgc ggcggaattt gccgacgtca acggcgacgg
     2281 aaaaatcgat ttatacgtcg ccaacgatgt cggcgaaaaa gcgatgttca taaatcgagg
     2341 cgataaattc gtcgacgaag cgatggagcg cggcgtcctc gacccgggca acggaatggg
     2401 cgtctctttc ggcgattaca acaacgacgg tcggctcgac attcacgcct cgaatatgag
     2461 ctcgaccgcc ggaaaccgca ttttgaccag actttttccg aattcgagcg cgaaagacaa
     2521 cgttttgaaa aagctcgccg ccggaaacaa tctttttgaa aatacgggcg acggaaaatt
     2581 taaagacgtg acggcggaag tcggcggatt ttcgggcggc tgggcttggg gcggcggttt
     2641 tatagacttc gacaacgacg gctgggaaga catctacacg ccgaacgg
//
LOCUS       DASWNE010000783         2697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_309549, whole genome shotgun sequence.
ACCESSION   DASWNE010000783 DASWNE010000000
VERSION     DASWNE010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309549"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..1688
                     /locus_tag="VGB68_15200"
     CDS             93..1688
                     /locus_tag="VGB68_15200"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEY0050638.1"
                     /translation="MEYAATTFNLASILEQHARLAPEKEAVVWNEMRFTYGQLDGLAN
                     RVANALTEMGIGYGDKVALACPNLPFFPIVYYGIVKTGAIIVPVCILFKSREVEYHLR
                     DSDAKAVFTFEGTPEFPLGNIVKEGFDRVEACEHLVVMTAKPTAPSPFPEHKTLTEIT
                     AAQPETFETYPTKPEDTCAILYTSGTTGQPKGAELTHFNLITNITTAYSIHLPVLDFT
                     EGRQKTCLITLPLFHTTGQTMQMNTNIYAGNRIVLLPKFEPQATLEAMEKEKVNFWVG
                     VPTMYWALLRYVEETGYDVSRIRENMKVPTSGGAPMPVEVMKEFEQVFGLRILEGYGL
                     SETSPLACFNHFEKPSKPGTVGQAIFGVEVRCVDETDAEVPRGTRGEVVIRGSNVMKG
                     YYKRPAATAEAFRNGWFHTGDIGIMDDEGYLSIVDRKKDMIIRGGYNVYPRELEEIIM
                     THPAVSLVAVIAVPDERLGEEVKAFIVRKPGATLTEEEMIEWCKKQFAANKYPRHIEF
                     RDELPVGNTGKILKRALREEKEK"
     gene            1778..2017
                     /locus_tag="VGB68_15205"
     CDS             1778..2017
                     /locus_tag="VGB68_15205"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEY0050639.1"
                     /translation="MKVQVLFFGATAQATGEREIEFDLAERTKAAEAFRQIVRRFPQL
                     EQHSLLFAVNQQYASGGETIQNGDELAVFTAVSGG"
     gene            2081..2512
                     /locus_tag="VGB68_15210"
     CDS             2081..2512
                     /locus_tag="VGB68_15210"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEY0050640.1"
                     /translation="MTNDFFELTGEPLDVGAIARRVVLPECGATVTLDGYVRRFTKGR
                     ETLYLVYEAYQAMALSEMEKLCERAREQFEIAHVGIVHRLGKLEIGETSVVISVSAPH
                     RRAAFEACEWVIKELKRTVPIWKKEVYADGETWVEGDSRKL"
BASE COUNT          680 a          681 c          732 g          604 t
ORIGIN      
        1 ggaagctgca aatagttgag agttgcagaa actttacaaa tcgttaaaat ttaaactgtc
       61 tgcaactttc tgcaagctct ggaaacttat ttatggaata cgctgcaacg acgtttaatc
      121 tggcttcgat tctggaacaa cacgctcgtc ttgcgccgga aaaggaagcc gtcgtctgga
      181 acgagatgcg ttttacatac gggcagctcg acgggctggc gaaccgcgtg gcgaacgctt
      241 tgaccgagat gggcatcggc tacggcgaca aagtcgcgct ggcttgcccg aatctgcctt
      301 tttttccgat tgtttattac ggcatcgtca aaaccggcgc gattatcgtg ccggtttgca
      361 tattattcaa atcgcgcgaa gtcgaatatc acctgcgcga ttccgacgcg aaagccgttt
      421 ttacttttga aggaacgccg gaatttccgc tcggaaacat tgtcaaggaa ggcttcgacc
      481 gcgtggaagc gtgcgagcat ctggttgtga tgaccgctaa accgaccgcg ccgagtccgt
      541 ttcccgaaca caagacttta accgagatta cagcggcgca gcccgaaact tttgaaactt
      601 atccgaccaa gccggaagac acttgcgcga ttctctacac ttccggcacc accggacagc
      661 cgaaaggcgc agagctgaca catttcaacc tgataacaaa tattacgacc gcctacagca
      721 tacatcttcc ggttctggat tttaccgaag gcaggcagaa aacctgcctg ataacactgc
      781 cgctgtttca cacgaccgga caaaccatgc agatgaacac caatatttac gccggaaacc
      841 gcatcgttct gctgccgaaa ttcgagccgc aggcaacgct cgaagcgatg gaaaaagaaa
      901 aggtgaattt ctgggtcggc gtgccgacga tgtattgggc gcttttgaga tacgtcgagg
      961 aaaccggcta cgacgtcagc cgcatccgcg aaaatatgaa agtcccgact tcgggcggcg
     1021 cgccgatgcc cgtcgaagtg atgaaggaat tcgagcaggt tttcggcttg cgtattctcg
     1081 aaggctacgg tttatcggaa acttcgccgc tcgcctgttt caatcatttc gaaaagcctt
     1141 caaagccggg aacggtcggg caggcgattt tcggcgtcga ggtcagatgc gtcgatgaaa
     1201 cggacgcgga agttccgcgc gggacacgcg gcgaggtcgt cattcgcggc tcaaacgtga
     1261 tgaaaggcta ttacaaacgt cccgctgcga ccgccgaagc gtttcgcaac ggctggtttc
     1321 acacgggcga catcggcatt atggacgacg aaggctatct gtcaatcgtc gaccgcaaaa
     1381 aagatatgat tattcgcggc ggctataacg tctacccgcg cgagctggaa gaaataatta
     1441 tgacgcatcc ggcggtctcg ctcgtcgccg tcatcgccgt tccggacgaa agactgggcg
     1501 aagaagtcaa agcgtttatt gtcagaaaac cgggcgcgac cctcaccgaa gaagaaatga
     1561 ttgaatggtg caaaaaacag tttgcggcga ataaataccc gcgccacata gagtttcgcg
     1621 acgagctgcc ggtcggcaac acgggaaaaa ttttgaaacg cgcgttgcgc gaggagaagg
     1681 aaaagtgaaa aagtgaaaag gcgaaaaagt tttattgatt tactgctcaa ctcgcctttt
     1741 tcactttttc accttttcac tttttcactc tatttttatg aaagtgcagg ttttattttt
     1801 cggcgcgacg gcgcaggcga ccggcgaaag agaaatcgaa tttgatttgg ctgaacggac
     1861 gaaagctgcc gaagctttcc ggcagattgt ccggcgattt ccgcagctcg aacaacattc
     1921 gctgctgttc gccgttaatc agcaatacgc gagcggcggc gagacgattc aaaacggcga
     1981 cgagctggcg gtttttacag ccgtttcggg cggctagata catttatcat ccggcattta
     2041 agatttaccg aaacgtccga tgatatgtgc taagtgataa atgacaaatg attttttcga
     2101 actcaccggc gaaccgctcg atgtcggcgc gattgcgcgc agagtcgttt tgcccgaatg
     2161 cggcgcgacc gtcactctgg acggatacgt gcggcggttt acaaaaggac gggaaacgct
     2221 ttatctggtt tacgaagcgt atcaggcgat ggctttgagc gagatggaaa agctctgcga
     2281 gcgggcgcgc gagcagtttg aaatcgcgca cgtcggcatc gttcatcgcc tcgggaagct
     2341 ggaaatcggg gaaacgagcg tggtcatttc ggtttccgcg ccgcatcgcc gcgccgcttt
     2401 cgaagcctgc gaatgggtga ttaaggagct gaaacgcacc gtcccgattt ggaaaaagga
     2461 agtttacgcc gacggggaaa cttgggtcga aggcgacagc cggaaacttt agatttcgga
     2521 ttgaataagg aagatttaat ttatcgtacc gggctggaag ccgaataagc ttcaatccaa
     2581 aatcgaaaat cgtcaattgc cgaacttttg ccgggctttt tccgccagct catcgaatcg
     2641 ccgttccagt tgagcgttcg cgccgcgcaa gcccgccacc actttttcgc cccattc
//
LOCUS       DASWNE010000784         1877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_377644, whole genome shotgun sequence.
ACCESSION   DASWNE010000784 DASWNE010000000
VERSION     DASWNE010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377644"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VGB68_15215"
     CDS             <1..442
                     /locus_tag="VGB68_15215"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0050641.1"
                     /translation="YEHFGSFQDKPIIGDFDGDGKSDLTVFREGVWYQMRSQLGFYAE
                     RFGLASDKPIAADYDGDGKTDIAVFRPGNGFWYLQRSTAGFTAMQFGTSNDKPVPADF
                     DGDGKTDIAIHRDDRWYLQQTSSGFRVVQLGLPGDMPIHTAFLP"
     gene            696..1328
                     /locus_tag="VGB68_15220"
     CDS             696..1328
                     /locus_tag="VGB68_15220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0050642.1"
                     /translation="MNEPDTANAPAPRLFLGRTRAGNIWRFRADVPEKLCARLDALCA
                     DEPPVKAEFKVPPRHFETYVRLLESAAPVREVSNGLAYQFAESELPSERRLIAVTENN
                     PEILQGDFEKLVEELPDWQPFVALVEEIGAVSVCRSVRITPEAHEAGVETLPDFRGKG
                     YAKDVVLEWARLVRAAGANPLYSTSWENTSSQALARKLRLKCYGTNFSVT"
     gene            complement(1667..>1877)
                     /locus_tag="VGB68_15225"
     CDS             complement(1667..>1877)
                     /locus_tag="VGB68_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050643.1"
                     /translation="LKLYSGTQFNQMLSAARNGQAGDERIMSSKHNWKYILIYPAWNL
                     VTFGAEEISFVEETLCFCLLPFSKY"
BASE COUNT          457 a          448 c          494 g          478 t
ORIGIN      
        1 atacgaacat ttcggcagct ttcaggacaa accgattatc ggcgatttcg acggcgacgg
       61 aaaaagcgat ttgaccgtct ttcgtgaagg cgtctggtat cagatgagaa gccagctggg
      121 attttacgcc gagcggttcg gcttagcttc ggataaacct atcgcggcgg attacgacgg
      181 cgacggcaaa accgatatcg ccgtttttcg acccggaaac ggcttttggt atttacagcg
      241 ctcgaccgcc ggttttacgg cgatgcagtt cgggacgtca aacgataaac cggttccggc
      301 ggattttgac ggcgacggca agacggacat tgccatacac cgcgacgacc gctggtatct
      361 gcaacaaaca tcgtcgggtt ttcgcgtcgt gcaattgggt ttgccgggcg atatgccgat
      421 tcacacggcg tttcttccgt aacgtaaaga gcaataaaaa taagcgctcc ggacaaatat
      481 tttcaatccg tttcgccttt tgtctctgaa aagcgggcga tacgacaatt tgttttttcg
      541 gcagattgct ctcggcacgc tcgaccgccg gcgcgtaaat gcgagatttg tttgccgtat
      601 aataaaaaaa cggttgtatg ctgactgatt tcgagttaat gaacattcac gttcgggctt
      661 tattcactca cgatgctgat tcgcggcttt tgtttgtgaa tgagccggat accgcgaatg
      721 ctcctgctcc tcgtctgttt ttgggtcgaa cgcgggcggg caatatttgg cggtttcgcg
      781 ccgatgtccc ggaaaaactc tgcgcgcggt tggacgcgct gtgtgcggac gaaccgcccg
      841 taaaggctga gttcaaagtg ccgccgcgtc atttcgagac atacgtccgg ctgctcgaaa
      901 gcgccgcgcc ggtgcgggaa gtgtcgaatg gcttggcata tcaatttgcc gaatccgagc
      961 tgccttcgga gcgtcggctt atcgccgtca cggaaaataa tccggagata ttacaaggcg
     1021 attttgaaaa attggttgaa gagcttcccg actggcagcc ttttgtcgcc ctcgtcgagg
     1081 aaatcggagc cgtgtcggtc tgtcgcagcg ttcgcattac gccggaagct cacgaggctg
     1141 gcgtcgagac gctgccggat tttcgcggga aagggtatgc caaagatgtc gtgcttgagt
     1201 gggcgcgatt ggtgcgggct gccggcgcaa atccgttgta cagcacgtcc tgggaaaata
     1261 cttcttcaca ggcattggcg cgaaagctgc gcttaaaatg ttacggcact aatttcagcg
     1321 tgacttaaca atcagttgga tttccgacgg tttgtcgttg tgtgacgcgc gatataaaag
     1381 gtcattgagc gcgcagcgtg cgagcagcgt ccttgttatt tagcctgcgt gtttttagct
     1441 ttgcttatcc gcgacaggct cgctcaaatc atcgcaatcg ttctgcttct caaaaatgtt
     1501 ctttcagagc gctaaccttt ttccttattc gtttcgaaaa cgataaagtc tttggaatag
     1561 acataaaacc ccgaaaccca gacacgcgcc taaagtgttt agaagaatgt catcaatatc
     1621 cacaacccta aaattgccga cgaatctgga aaaaaattga attgcttcaa tacttagaga
     1681 aaggcaaaag gcaaaaacac aaagtctctt ccacgaatga aatctcttca gcgccaaagg
     1741 taacaagatt ccaagcggga taaataagaa tatatttcca attatgtttt gatgacataa
     1801 tacgctcatc tccggcttgc ccgtttcgtg cggcagaaag catctgattg aattgagtgc
     1861 cggaataaag tttaagt
//
LOCUS       DASWNE010000785         2629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_96222, whole genome shotgun sequence.
ACCESSION   DASWNE010000785 DASWNE010000000
VERSION     DASWNE010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96222"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..585)
                     /locus_tag="VGB68_15230"
     CDS             complement(55..585)
                     /locus_tag="VGB68_15230"
                     /inference="COORDINATES: protein motif:HMM:NF024712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4087 domain-containing protein"
                     /protein_id="HEY0050644.1"
                     /translation="MPKFLSYFLILFFLSFSISSCRSVRQVDISSNASNAAAQNSKKE
                     PQQKAEAVHVAPKEANADEQKKSSADSATTSAGGKFENRCGWFSNPTPANAWLEDRDG
                     EWIIGIQGGHQAEGDYPEFDDDKWIKTNVNYGYGCACMKVKVDYKSHRVLEIASATAK
                     PLSACRQDPALKEPVN"
     gene            complement(642..1136)
                     /locus_tag="VGB68_15235"
     CDS             complement(642..1136)
                     /locus_tag="VGB68_15235"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY0050645.1"
                     /translation="MKLIAELNNEKHEVEFKRDGEKISASVGGREYALEASEVEPNVY
                     LLKHENQIFQIYVSPSETPAVTLGDHSFEIKIADPKRLRGSGALAGNADGTVEIKTAM
                     PGKVVRILAEQGAEIKQGESVLVVEAMKMQNEMKSPKDGIVKEIRVAEGATVNAGDVL
                     AIIE"
     gene            complement(1145..2269)
                     /gene="speY"
                     /locus_tag="VGB68_15240"
     CDS             complement(1145..2269)
                     /gene="speY"
                     /locus_tag="VGB68_15240"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF001980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HEY0050646.1"
                     /translation="MAKKKSRFLSGKRIDPTPIDKNTALADLVDESFMAYNAARLREA
                     CQLFTKKMLEPDVTIGVTLTGALTPAGLGISALIPLIQAGFIDWIISTGANLYHDTHF
                     GIGLSLHQGDAKLDDRVLREEEVVRIFDIFFDYSVLLDTDEFFRRLIEGEEFQKTMSS
                     AEFHYLCGKYIRERENKLGIKNKSLLATAYEYGVPIYTSSPGDSSIGMNIAAKELQGG
                     KLILNPNLDVNETASIVLAAKRGEIHGDKEEGGKSAIFILGGGSPKNFALQTEPQIQE
                     VLGIDEKGHDYFLQVTDARPDTGGLSGATPAEAVSWGKVDPDKLPDAVVCYVDSTVAL
                     PILTAYALARHERREPKRLYDRRDEFMNLLKREYEKSTRR"
BASE COUNT          575 a          673 c          663 g          718 t
ORIGIN      
        1 attttccgtg ttcaaatgcg ttgaaaaatc tttcatcgaa taaaacgtat ctttctaatt
       61 aaccggctct ttcagcgccg ggtcctggcg gcaagccgaa agcggtttag ccgtggcgct
      121 cgcgatttcc agaactctgt gagatttata atcgacttta actttcatac acgcgcagcc
      181 gtatccgtaa ttgacattgg tctttatcca tttatcgtcg tcgaattcgg gataatcgcc
      241 ctcggcttga tgtccgccct gaatgccgat aatccattcg ccgtcgcggt cttcgagcca
      301 ggcgttggcg ggcgtcgggt tgctgaacca gccgcagcgg ttttcgaatt tcccgcccgc
      361 ggaagtcgtc gccgaatcag ccgaagattt cttttgctcg tcggcgttcg cttctttcgg
      421 tgcgacgtgg acggcttcgg ctttttgctg cggttctttt tttgaatttt gcgccgccgc
      481 atttgaagcg ttagacgaaa tatcgacttg ccggacgctt cggcaggacg aaatcgaaaa
      541 gctgagaaag aaaagtataa ggaaataaga caaaaatttc ggcatatttt tattttgaaa
      601 tctcaccggc aagcaaaaaa gtctctggaa aagaaacaat tttactcgat aatcgccagc
      661 acgtcgcccg cgttgacggt cgcgccttcg gcgacgcgaa tttctttgac gatgccgtct
      721 ttcggcgatt tcatttcgtt ctgcatcttc atcgcctcga cgaccagaac gctttcgccc
      781 tgtttgattt cggctccctg ttcggcgaga atgcgcacga ctttgccggg catcgcggtt
      841 tttatttcga ccgtgccgtc ggcgtttccg gctagagcgc ccgagcctcg caggcgtttc
      901 gggtcggcga tttttatttc aaacgaatga tctccgagcg tgacggcagg cgtttcggac
      961 ggcgaaacgt aaatctgaaa aatttgattt tcgtgtttta aaagataaac gttcggctcg
     1021 acttccgacg cttccagcgc gtattcgcgc ccgccgaccg aagcggaaat cttctcgccg
     1081 tcgcgtttaa attcgacttc gtgtttttcg ttgtttagtt cggcaattag tttcattgtt
     1141 ggaattatct tcgcgtcgat ttttcgtatt ctctcttaag taaattcata aattcatcgc
     1201 gccggtcgta aagccgtttc ggttcgcgcc gctcgtgccg cgccagcgcg taagccgtca
     1261 gaatcggcag agcgacggtc gaatcgacat agcagacgac cgcgtcgggc agtttgtccg
     1321 ggtcgacctt gccccagctg accgcttcag ccggagtcgc gccgctcaat ccgcccgtgt
     1381 cggggcgcgc gtccgtcacc tgcaggaaat aatcgtgacc tttttcgtcg atgcccagaa
     1441 cttcctgaat ctgcggctcg gtttgcagcg cgaaattttt cgggctgccg ccgccgagaa
     1501 taaatatcgc ggattttccg ccttcttcct tatcgccgtg aatttcgccg cgttttgccg
     1561 ccagcacgat ggaagcggtt tcgttgacgt ccaggttcgg attcaggatg agctttccgc
     1621 cctgcagctc cttggcggcg atgttcatcc cgattgacga atcgccgggc gaagacgtgt
     1681 aaatcggaac gccgtattca taagcggtcg ccagcagcga tttgtttttg atgccgagct
     1741 tgttttcgcg ctcgcggatg tattttccgc acaggtaatg aaactccgcc gagctcatcg
     1801 ttttttgaaa ttcttcgcct tcgataagac ggcggaaaaa ttcgtcggtg tcgagcaaga
     1861 ccgagtaatc gaaaaagatg tcgaaaatcc gcacgacttc ttcctcgcgc aaaacgcggt
     1921 cgtcgagctt cgcgtcgccc tgatgcaggg ataagccgat gccgaaatgc gtgtcgtgat
     1981 aaagattagc gcccgtcgag ataatccagt cgataaatcc ggcttgaatc aacggaatca
     2041 gcgcggaaat tccaagtcct gcaggcgtca gcgcgccggt cagagtgacg ccgatagtga
     2101 cgtccggctc aagcattttt ttggtaaaaa gctggcacgc ctcgcgcaga cgcgccgcgt
     2161 tatacgccat aaaagattcg tcgaccaaat ccgccagagc cgtattttta tcaatcggcg
     2221 tcgggtcaat ccgcttgccg ctcaaaaacc tgcttttctt tttcgccata gaaagattat
     2281 tttggaattt gcgaaaaatt gcaaagcaaa aggctgcaaa atattttgca gcctttcagg
     2341 aaaattcgac gcggctcgag ttttgttaaa cagtatttta cctgcaagat gactctttta
     2401 cgcgcaagat gactctttta cacgcaagtt cggactttta ggcgcaagat tgaactttta
     2461 cgcgcaagat tggactttta cgcgcaagat gaagcttttg cctgcaatac cgaacttttg
     2521 cgcgcaagat gaaactttcg ctcgaaatac gacattttca gtttaaagct gattttaata
     2581 aagaatttgc ggaaggtttg ttattcggtg gtcaacgatg tggtttcgt
//
LOCUS       DASWNE010000786         3654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_237708, whole genome shotgun sequence.
ACCESSION   DASWNE010000786 DASWNE010000000
VERSION     DASWNE010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237708"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(311..1486)
                     /locus_tag="VGB68_15245"
     CDS             complement(311..1486)
                     /locus_tag="VGB68_15245"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEY0050647.1"
                     /translation="MSDTELNFQDTKTAFADKSDAELKEKYRLFKMMNSPVLTDFGTS
                     AAQMALRLGLPVKGLIKSTVFKQFCGGETIEECQPTIDKLGESNIGAILDYSIEGKSE
                     EAVFDSTKNEIYRTVTRAKEDDAIPFAVFKVTGLARVELLEKVGSDKELFVGEQEEWD
                     KAKARVEEICEYAHSLEQPVFIDAEESWVQDAIDALANEMMEKYNREKPVIYNTIQLY
                     RHDRLEFLKQSHRKAKADGYILAVKLVRGAYMEKERTRAAEMDYPSPIQPDKEATDRD
                     FDAALEYCVENVEQIAFVAGTHNEKSIQLLTQLMDKHKVEPSHPHVNFSQLYGMSDNL
                     SYVLAKNGYCVSKYVPYGPVKDTIPYLIRRAQENTSVVGQMSRELDLISRELKRRKV"
     gene            1711..3183
                     /gene="proS"
                     /locus_tag="VGB68_15250"
     CDS             1711..3183
                     /gene="proS"
                     /locus_tag="VGB68_15250"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEY0050648.1"
                     /translation="MSKGLPKRSENYSEWYNEIVKRAELAENSAVRGCMVIKPYGYAI
                     WEKMQRALDDMFKATGHQNAYFPLFVPKSFLEKEEEHAEGFAKECAVVTHYRLKPNPE
                     GKGLIVDPNAKLEEELIIRPTSEAIIWNAYKGWIQSWRDLPLLINQWANVVRWEMRTR
                     IFLRTAEFLWQEGHTAHATEAEAIEESEKMLGVYATFAEDWMALPVLKGIKTPNERFA
                     GAEETYCIEGLMQDGKALQAGTSHFLGQNFARSFDVQFTNREGKLEYAWATSWGVSTR
                     LMGALIMAHSDDNGLVLPPKLAPIQVVIVPIYKSEEDLAKISEKVLPVFEELKARGIS
                     VKYDDDDKQRSGWKFAEYELRGVPVRLGIGLRDLENGTIEVARRDTLTKESRSLEGVA
                     GYVADLLDEIQTSIYQKALNFREQNTFRIDTWEDFNTQIEKGGFISAHWDGSGETEEK
                     IKQETKATIRCIPLDAPEEAGACVYSGKPSNRRVIFARAY"
BASE COUNT          906 a          891 c          897 g          960 t
ORIGIN      
        1 acaaaacaat caaaaacatt gcgaaaaccg caatcatcga tgacgttaaa aattttttca
       61 ttttttctct ctccttattc tattaccgga aaccggcaat ctgttttcaa gttttagact
      121 ctcaaacgac ctgaacggtc ggaaaatttc ggggagagtg acgaaagctt tgaatatttc
      181 gaggcggaaa cggttgtccg gaattagcga ttagccgttt ggctgccggc gaaagatttg
      241 tacgcggcag gcgtttaatg ctcaaagccg ctcgccgaag gcgaacgtct aatcgccgtc
      301 gctccgagcc tcaaaccttt cggcgtttca attcccggct gatcaaatcg agctcgcggc
      361 tcatctgacc gacgaccgaa gtgttttcct gcgcgcgccg aatcaggtac ggaatcgtat
      421 ctttgaccgg accgtaaggc acgtatttcg agacgcagta accgtttttc gccagaacgt
      481 aggaaagatt gtcgctcatc ccgtaaagct gtgaaaaatt gacgtgcggg tgtgacggtt
      541 cgactttgtg cttgtccatt aactgcgtca gaagctggat gcttttctcg ttgtgcgttc
      601 cggcgacgaa agctatttgc tcgacattct cgacgcaata ttccagagcg gcgtcaaaat
      661 cccggtcggt cgcttcctta tcgggctgaa tcggcgacgg gtaatccatc tcggcggcgc
      721 gcgtccgttc tttttccata tacgcgccgc gcacgagctt gacggcgaga atgtaaccgt
      781 ccgctttcgc cttccggtgc gattgtttca gaaattccag acggtcgtgg cggtaaagct
      841 gaatcgtatt gtaaattacc ggcttttcgc ggttgtattt ttccatcatt tcattcgcca
      901 gcgcgtcgat ggcgtcctgc acccaggatt cttcggcgtc gataaaaacg ggctgttcga
      961 gcgaatgcgc gtattcgcag atttcttcga cgcgcgcttt cgccttgtcc cattcttcct
     1021 gctcgccgac gaacagctct ttgtccgaac cgactttttc cagcagctcg acgcgcgcca
     1081 agcccgtcac tttaaaaacg gcgaaaggaa tggcgtcgtc ttctttggcg cgcgtgacgg
     1141 tgcggtaaat ctcgtttttc gtgctgtcga aaacggcttc ttcggatttg ccttcgatgg
     1201 aataatcgag aatcgcgccg atgttcgatt cgcccagctt atcaatcgtc ggctggcatt
     1261 cctcgatggt ttcgccgccg cagaattgtt tgaaaaccgt gctttttatc aagcctttga
     1321 ccggcagccc gagccgcaga gccatctgcg ccgcgctcgt gccgaagtcg gtcagaaccg
     1381 gcgagttcat cattttgaaa agacgatatt tttccttgag ttccgcgtcc gatttatcgg
     1441 caaaagcggt tttcgtgtcc tgaaaattca attcggtatc gctcattttt ttgcttttag
     1501 caatcagccg tcagctttca gctttcagct tttttgtttt cctcaaaatt atttactaat
     1561 tgaattatac acaaacgaaa gctcgatggc ggataaattc aactaatttt aaatgcgaaa
     1621 ttgtttgttt tcttattttg ctttatcatt tttcctttac ttgaaataca aaaagctgac
     1681 cgctgacagc tgacagctaa tagctaattt atgagcaaag gtttaccgaa acgaagcgaa
     1741 aattattcag aatggtataa cgaaatcgtc aagcgggcgg agctggcgga aaactccgcc
     1801 gtgcgcggct gtatggtgat aaagccgtac ggctacgcga tttgggaaaa aatgcagcgc
     1861 gcgctcgacg atatgttcaa ggcgaccggg catcaaaacg cctatttccc gctgttcgtg
     1921 ccgaaatctt ttctggaaaa agaagaggaa cacgccgaag gctttgccaa agaatgcgcc
     1981 gtcgtgacgc attatcgtct gaaaccgaac cccgaaggca agggcttaat cgtcgacccg
     2041 aacgcgaaac tcgaagaaga gcttatcatt cgcccgacgt cggaagccat catctggaac
     2101 gcttataaag gctggattca atcgtggcgc gacctgcctc tgctcatcaa tcaatgggca
     2161 aacgtcgtgc gctgggaaat gcggacgcgt atctttttaa gaacggcgga atttctgtgg
     2221 caggaaggac acacggcgca cgcgaccgaa gccgaagcaa tcgaagaaag cgaaaaaatg
     2281 ctcggcgttt acgcgacctt tgccgaagat tggatggctc tgcccgttct gaaaggaatc
     2341 aagacgccga acgaaagatt tgccggcgct gaagaaactt attgcatcga aggcttgatg
     2401 caggacggaa aagccctgca agccgggact tcgcactttt taggacaaaa tttcgcgcgc
     2461 tctttcgatg tgcagtttac aaacagagaa ggcaagctcg aatacgcctg ggcgacgtcg
     2521 tggggcgttt cgacgcgtct gatgggcgct ctgattatgg ctcattccga cgacaacggc
     2581 ttggttctgc cgccgaagct cgctcccatt caggtcgtga ttgtgccgat ttataaatcg
     2641 gaagaagatt tggcgaagat ttccgagaaa gtcctgcccg tttttgaaga gctgaaagcc
     2701 agaggcattt cggtcaaata cgacgacgac gataaacagc gttccggctg gaaatttgcc
     2761 gaatacgagc tgcgcggcgt tcccgtccgg ctcggcatcg gcttgagaga tttggaaaac
     2821 ggaacgattg aagtcgcgcg ccgcgacact ctgacgaaag aatcgcgttc gttggaagga
     2881 gttgccggtt atgtggcgga tttgctcgac gaaattcaaa cgagcatcta tcaaaaagcg
     2941 ctgaatttcc gtgagcaaaa cactttccgc attgatacct gggaagattt caacacgcaa
     3001 atcgaaaaag gcggctttat ctcggcgcac tgggacggca gcggcgaaac cgaagaaaaa
     3061 atcaagcagg aaaccaaggc gacgattcgc tgcattccgc tcgacgcgcc ggaagaagcg
     3121 ggcgcgtgcg tttattcggg caaaccgtca aacaggcgag tcattttcgc gcgcgcttat
     3181 taaattattg gacgagcgaa agtaggaaac ctttgttagg ttttaaggga aaaatctgta
     3241 aatttcccta cggtgaagaa atctaatgaa atatacaatt tcgttttcga ttttcagacc
     3301 gaaccggtaa tctcccaacc taatacgaaa gtagtcctga tgccctcgca gttttttaca
     3361 acctgctatt gattctgtag tttccgcaat ttcgacactt tcaattattt tcttgattcg
     3421 ttgacgcaat gtcttatctc ttaccggtcg taagtctttt aaaaaacttt cgcggaactc
     3481 tactttcaat ttttgttctc caaaaggtca aaaatctctg cgcgcgaaac caaagatgtt
     3541 tgctcgcctt ctttaatcgc ttgcagcaat ccgatttttt ctttttcctc tgagactcta
     3601 tccggaaaat ctctattttt tctgacgatt tcaaaaatcg tttttgaaga gtaa
//
LOCUS       DASWNE010000787         3313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_662687, whole genome shotgun sequence.
ACCESSION   DASWNE010000787 DASWNE010000000
VERSION     DASWNE010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662687"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..712
                     /gene="tsf"
                     /locus_tag="VGB68_15255"
     CDS             44..712
                     /gene="tsf"
                     /locus_tag="VGB68_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEY0050649.1"
                     /translation="MAEITASAVKSLREKTGAGMVDCKNALVEASGDESAAVEILRKK
                     GMATASKKQGRVTAEGAVGSYIHMGGKVGVLVEINCESDFVARGEEFQQLVKDVAMHI
                     AAVDPRFVSREEVPTEVLQKEREILLEQLKNDPKNANKPEDVLNKIIEGRLNKFYEES
                     VLIDQPFVKDPSKTIGELVTEKISSIKENISIRRFARFKMGEGIEKKQDDFASEVASM
                     VGGN"
     gene            772..1518
                     /gene="pyrH"
                     /locus_tag="VGB68_15260"
     CDS             772..1518
                     /gene="pyrH"
                     /locus_tag="VGB68_15260"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEY0050650.1"
                     /translation="MSTSAVESAKPAFQRILLKLSGEALMGAQSYGIDTNVAESVARE
                     IKAIYDLGIEIAIVVGGGNIFRGVSKSAGNMDRGSADYIGMLATVMNGVVLQDALERI
                     GVPTRVMSAIDIPELAEPFIRRRALRHLEKNRVVIFAAGTGNPYFTTDSAAALRALEI
                     KAEVIFKATKVDGIYTEDPFINPRATRFESISYQEVLEKQLKVMDSSAISLCMDNKLP
                     IMVFNMKESGNIIKAVKGDLTIGTLVGGNA"
     gene            1552..2112
                     /gene="frr"
                     /locus_tag="VGB68_15265"
     CDS             1552..2112
                     /gene="frr"
                     /locus_tag="VGB68_15265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEY0050651.1"
                     /translation="MSAEDVIKETRPRMEAVVEDFKRKLGNVRTGRATVGLLDSITVD
                     YYGTPTPLSQMASIAVPEPQLLTVQPWDMSQVGAIEKAILAANLGFNPSNDGKIVRLP
                     IPPLNEERRRQLAKQVHEIAEEHRVAVRNVRHSSNDTLKKLLKDKAISEDSERDGLDQ
                     IQKLTNTFVSKIDELAKTKEQEIMHV"
     gene            complement(2238..2993)
                     /locus_tag="VGB68_15270"
     CDS             complement(2238..2993)
                     /locus_tag="VGB68_15270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050652.1"
                     /translation="MKKRYLLPLVLISAIGVSAQVEQKKNEPKTDKTTTTVSGVKTDA
                     SSLDLAKATLAAHGGEKLIKIKNIVVRGTAELSAGASAQTMPAAFAITISGARFRYDI
                     QSQFFNYKQIYDGQQNYSSISSFSLPLDKVGMELLRKINDREYTVSALPEKWKKKKGF
                     RITSPEGFYTDFVVDEKTNQVKEYESSYEVNGETLTTSVAVDKYREIDGVFINEKFSQ
                     RMELSQGTFYANFKAKDILINSEIADDVFAMPK"
     gene            complement(3104..>3313)
                     /locus_tag="VGB68_15275"
     CDS             complement(3104..>3313)
                     /locus_tag="VGB68_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050653.1"
                     /translation="GVSDSPYNLTLGIQFSNLLNRNNRGIPVGSLNSRLFGESVSTGG
                     SFGFFGGGGSSSGNRRVELQARFSW"
BASE COUNT          885 a          792 c          853 g          783 t
ORIGIN      
        1 aagctgaccg ctgacggctg attgctaaca gatggagatt attatggcag aaattacagc
       61 aagtgcagta aaatccctgc gcgaaaaaac cggcgcggga atggttgatt gtaaaaacgc
      121 actggtcgaa gccagcggcg acgagagcgc ggcggtggaa atccttcgta aaaaaggaat
      181 ggcgaccgcc agcaaaaaac agggacgcgt gacggcggaa ggcgcggtcg gctcctacat
      241 tcatatgggc ggaaaagtcg gcgtgctggt cgaaatcaac tgcgaatcgg atttcgtcgc
      301 gcgcggcgaa gaatttcagc agctcgtcaa agacgtggcg atgcacatcg ccgccgtcga
      361 cccgcgcttt gtcagccggg aagaagttcc gactgaggtt ttgcagaaag agcgcgagat
      421 tttgctcgaa caattgaaaa acgacccgaa aaacgcgaac aagcccgaag atgttctgaa
      481 caaaatcatc gaaggtcgcc tgaacaagtt ttatgaagaa tcggttttaa tcgaccagcc
      541 tttcgtcaaa gacccttcga aaacaatcgg cgagctggtg acggaaaaaa tttcctcgat
      601 taaggaaaac atctcgattc gccgttttgc gcgtttcaag atgggcgaag gcatcgagaa
      661 gaaacaggac gatttcgcat cggaagtcgc ttcgatggtc ggcggaaatt aatttttcag
      721 caaaaggctc aaggttaaaa actttgagcc ttttttgtat agtattaaat tatgtcaact
      781 tcggcggtcg aatcagcgaa accggctttt cagagaattt tattaaagct ttcgggcgaa
      841 gccctgatgg gcgcgcaaag ttacggcatc gatactaatg tcgccgagtc ggtcgccaga
      901 gaaatcaagg cgatttacga tttgggcatc gagattgcga ttgtcgtcgg cggcggcaac
      961 atttttcgcg gcgtctcgaa atcggctgga aatatggacc gcggcagcgc cgattacatc
     1021 ggaatgctcg ccacggtgat gaacggcgtc gttttgcagg acgcgctcga acgaatcggc
     1081 gtgccgacgc gcgtgatgtc cgcgattgat attcccgaac ttgccgaacc gtttatacgc
     1141 cgccgcgccc tgcgtcattt agagaaaaac cgcgtcgtga ttttcgccgc cggaacggga
     1201 aatccctatt ttacgaccga ttccgccgcc gccttacgcg cgctggaaat caaggcggaa
     1261 gttattttta aagcgacgaa agtcgacgga atttacaccg aagacccgtt tatcaatccg
     1321 cgcgcgacgc gctttgaatc gatttcctac caggaagttt tggaaaagca gctgaaagtg
     1381 atggattcgt cggcgatttc gctttgtatg gacaataagc tgccgataat ggtttttaat
     1441 atgaaagaat ccggcaatat tatcaaagcc gtgaaaggcg atttgactat cggcactttg
     1501 gtcggaggca acgcttaagt tataatcttt attacgtttt acgaggaaaa tatgagcgca
     1561 gaagatgtaa tcaaagaaac cagaccgcga atggaagcgg tcgtcgaaga ttttaaacgc
     1621 aagcttggaa acgtccgcac gggacgcgcg accgtcggct tgctcgacag cataacggtc
     1681 gattattacg ggacgccgac gcctctgagc cagatggctt cgattgccgt tcccgaaccg
     1741 cagcttttga cggttcagcc gtgggatatg tcgcaggtcg gcgcgattga aaaagcgatt
     1801 ctcgccgcca atttaggatt taatccctcg aacgacggca aaatcgtgcg gctgccgatt
     1861 ccgcctttaa acgaagaacg ccgccggcag cttgccaaac aggttcacga aatcgcggaa
     1921 gaacatcgcg tggcggttcg caacgttcgc cacagctcaa acgacaccct gaaaaaatta
     1981 ttaaaggaca aggcgatttc cgaagacagc gagcgcgacg gtttagacca gattcaaaaa
     2041 ctgacgaaca ctttcgtcag taaaatcgac gagctggcga aaaccaaaga acaggaaata
     2101 atgcacgtct gatttcagga acggacgcgg agacaaagaa agacgcgggg acgcggagat
     2161 ggtttgctaa tctcatactt gagatttaaa aacaaaattc tccgcgtcgc cgcgtcgccg
     2221 tttctctttg tcacaaacta tttcggcatc gcaaaaacat cgtccgcgat ttcggaattt
     2281 atcaaaatat cctttgcctt aaagttcgca taaaaagttc cctgcgacaa ttccatccgc
     2341 tgcgaaaact tttcgttgat aaaaacgccg tctatctcgc ggtatttatc gaccgccacc
     2401 gaggtcgtca gagtctcgcc gttaacctcg tagctcgatt cgtattcttt cacctgattg
     2461 gttttttcgt caacgacaaa atcggtgtaa aagccttcgg gcgaagtgat gcggaaacct
     2521 tttttctttt tccatttttc gggcagcgcc gaaaccgtgt attcccggtc gttgattttc
     2581 cgcagaagct ccattcccac tttatccagc ggcaggctga agctgctgat tgacgagtaa
     2641 ttttgctgac cgtcgtaaat ctgcttgtaa ttaaaaaact gcgattgaat atcgtaacga
     2701 aacctcgcgc ccgaaatggt gatggcgaag gcggcgggca tcgtttgcgc cgaagctccg
     2761 gctgacagtt cagccgttcc gcgcacgact atattcttga tttttatgag tttttctccg
     2821 ccgtgcgccg caagcgtggc ttttgccaaa tccagcgagg aagcatcggt tttaacaccg
     2881 gagacggtgg tggttgtttt atcggtttta ggctcgtttt ttttctgttc gacctgagcg
     2941 ctgaccccga tggcagaaat taaaacgagc ggcaaaagat agcgtttttt cattttgact
     3001 ccttaaattt taggaaagac ttattatccg taaaatacga aagatggaga atgaaagttt
     3061 aacttaattc attctccatc tttttaaccg attttagcgg aaattaccag ctaaaacgcg
     3121 cctgcaattc gacgcggcga ttgccggagc tcgaaccgcc gccgccgaag aaaccgaacg
     3181 aaccgcccgt cgagaccgat tcgccgaaca atctggaatt taagctgccg accggaatac
     3241 cgcgattgtt gcggttaagc aaattggaaa actgtattcc gagcgttaag ttatacgggc
     3301 tgtcgctgac gcc
//
LOCUS       DASWNE010000788        12460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_130712, whole genome shotgun sequence.
ACCESSION   DASWNE010000788 DASWNE010000000
VERSION     DASWNE010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12460
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130712"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VGB68_15280"
     CDS             <1..336
                     /locus_tag="VGB68_15280"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0050654.1"
                     /translation="NKILKMKFTIEEFLEKLPLPANEKWKDGVWDVEPFKKGNVTLVF
                     FAPRAADYQTFHEDDEFYFIARGNAELIVENERFACETGDCFFVPAQKKHHFENMSDE
                     FATWAIFFK"
     gene            complement(390..1013)
                     /locus_tag="VGB68_15285"
     CDS             complement(390..1013)
                     /locus_tag="VGB68_15285"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001886.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HEY0050655.1"
                     /translation="MLRLNKAHPWHGISIGDKVPNEVTVFIEIVPRDTVKYEIDKETG
                     YLKIDRPQQYSNVVPANYGFVPQTYCGQRVARLAKEKSGKEITLGDGDPLDILVLSEH
                     HIPRGDIILKATPIGGFCLIDNGEADDKIIATLVGDKIYENYKDLSELPKSIVNRLQH
                     YFLTYKTLPSEKSIIDIAYIYGREESYDVIGAAIEDYVELRAKLSEV"
     gene            1409..1981
                     /locus_tag="VGB68_15290"
     CDS             1409..1981
                     /locus_tag="VGB68_15290"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY0050656.1"
                     /translation="MSQSIIVEKSGKAAIIRFNRPAIKNPLSVETLENLEQIFTLLNF
                     DTTVKKIIFTGSGDTFASGANLNEVAALTTETAREFGLRGQNLMRKIYRAEKITFAAI
                     DGFCMGGALDLALSCRRRVASPRSIFAHPGASLGIITGWSGTQILPRLVGKKKAFEIF
                     LTAKKVSAQEALEIGLIDKISADPLQFATA"
     gene            complement(1996..4614)
                     /locus_tag="VGB68_15295"
     CDS             complement(1996..4614)
                     /locus_tag="VGB68_15295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEY0050657.1"
                     /translation="MATAIIKLDKSLSVKSFPKGKKTAVTETAPAAKIVNNRKSALKK
                     WIFSISLTLLFCSALAAIGFYYFYSQAAMIIDQRMKRGFWQTRSGIYAAPHVLKTGQK
                     IKTEEIVAILRHSGYVEGENRNIWNGNFTVKGDAVSIQTNHSAGDVPEIINLEISKGK
                     ISKIKNGNHALETYELQPEMISGNSEAKRDSINTLKFEQIPELLRNAIIYTEDRRFFE
                     HGGLDPRGILRAFSRNLSDNQISQGGSTITQQFVKNVFLSNEKTFGRKFSEAFLALAL
                     EKNLSKEDIFALYCNEIYFGQHGSIGIHGVEQASRSYFGKRIENLSLAEAATLAAMIK
                     SPHKYAPNRNYEAARERRNLVIQKLSEEGLISPQEAEKAQAEEIVLAAPAKNDKSIAP
                     YFVDAVHKEISGEKLPERNLRVYTTIDPQLQEMAEDSVNKKLAELDKTFAKKGVTPQA
                     ALVAIDPKNGHVLAMVGGRDYAASQLNRATEARRQPGSTFKPFVYATALEQGKLPANL
                     IYDQPTAFAIDRKPYKPANYGNSYAMHEITLKTALAKSSNVAAVEIALESGLQKVAGT
                     AEKFGMKRPEAYPSMALGTEEVTPLELAAAYAAFAGGGKRVNPVFVSQILSGEGNPVY
                     KNKNADTQIISPQTAYMITDMLGAVVERGTAQSAKDSLGKDVAFVGKTGSSKDGWFVG
                     YTPNLVCVVWIGFDENKDIGFTGGEVALPVWVDFMRKTTEARPEFGGSAFPMPKGLVE
                     VTIDPETGMLADDFCPQREKVVVPSKSFSNLECLRHQPKPDALYAQFQENETIAPENY
                     QTIRTETETTQNNRGVEQYKSINGIPVSRDEKNEKITVERNDKLKTGEEKFDSVEETG
                     DLRKSKKEATVGADGR"
     gene            complement(4828..5589)
                     /locus_tag="VGB68_15300"
     CDS             complement(4828..5589)
                     /locus_tag="VGB68_15300"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY0050658.1"
                     /translation="MSEILINQENSICYLTLNRPEKRNALNDALVNSLKDALRDADGN
                     RELRAIVIKGAGNDFCSGADLSALQKISESNVLENWDDAHNLMELFALIRKVKIPVVA
                     AVHGRALAGGCGLATACDLVLAGASARFGYPEVKIGFVPAMVMAILRRNVSEKRAFEL
                     ATQGFEFGAETAREFGLVNRIFEDSVFAEEVKNYVSIYEKVSRSAIVLSKRLLYQMDG
                     LTFESALESGVDVNTIARLTDDCQNGISRFLNKDK"
     gene            complement(5582..5935)
                     /locus_tag="VGB68_15305"
     CDS             complement(5582..5935)
                     /locus_tag="VGB68_15305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEY0050659.1"
                     /translation="MKDNPVLEKSLVFALRIVRLYRYLVEEKKEYVLSKELLISGTHI
                     GKHIKEAVNAESRQSFVHEMGVARRKASEAEYWLQLLHQGDYLSDKEFASIETDRAEI
                     FKLLTSIVKTSKENE"
     gene            complement(6032..8278)
                     /locus_tag="VGB68_15310"
     CDS             complement(6032..8278)
                     /locus_tag="VGB68_15310"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEY0050660.1"
                     /translation="MRNSFLRVFFVCLLAFSVVAQSTPNEKSLLGFSAAGSARQTALE
                     EKFDSYLRRENLRDWMKRLSARPHHVGSPYGKQNAEFMAALFKSWGFETRIEQFDVLF
                     PTPKTRLLEMTAPEKFTAKLIEPELKEDATSNQQSEQLPIYNAYSIDGDVSGDLVYVN
                     YGIPADYEELEKRGIDVKGKIVIARYGGSWRGIKPKVAAEKGAIGCLIYSDPRDDGYF
                     QGDVYPKGAYRNENGAQRGSVADMPLFPGDPLTPGVGSTKDAKRLDRKDAPTLTKIPV
                     LPISYGDALPLLRNLGGAVVPQSWRGALPITYHFGGANSLKVRLKLEFNWDIKPAYNV
                     IAKMNGAEFPDQWIIRGNHHDAWVNGADDPISGMVALMEEARAVGELAKTGWKPKRTI
                     IYAAWDAEEPGLLGSTEWAELHADELRQKAVVYINSDSNGRGFLGVGGSHTLEKFANE
                     VAKNVIDPQTKISVWQRLKALQAVNGSPNSRREALERGDLRIYALGSGSDYTPFLQHL
                     GIASFNIGFGGEDGGGSYHSVYDSFDHFTRFGDPNFDYGIALAKVCGRTTLRFANAEI
                     LPFEFTNFADTISTYVREVTTLADNLREETRMTNRMISDRMFAVVQDPAETFVAPKPK
                     ADVPFLNFAPLQNALAKLQESAKKYEAASKNKQLSPAAHKSLGEILYRAERALTRSEG
                     LPRRSWFKHQIYAPGFYTGYGVKTLPGVREAIEQRDWKEASEQIEIAARTIENFASEI
                     DKATNLIR"
     gene            complement(8457..9317)
                     /locus_tag="VGB68_15315"
     CDS             complement(8457..9317)
                     /locus_tag="VGB68_15315"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0050661.1"
                     /translation="MSEITFDYAQIGGVRLHYATAGEGERLVILLHGFPEFWYSWRHQ
                     LAALGDEYTVVAPDMRGYNLSDKPTGVSDYEIDKMADDVVGLIHHFGRERAAVIGHDW
                     GATVAWALASKHPEYLWKLGALQVPPVAVWRKNQTFRQFAASWYMFFFQIPLLPENLL
                     KLKDFALLENALKNSTAEKDVFTDEDIAKYKKTWSEPFALTAMLNYYRANIVKRFFTK
                     LEDTPKIKVPTVFIYGEKDKAVLPETVRGIGEMMDAPFEEFRIPTSGHWVQQEASEAV
                     TEILREFLAE"
     gene            complement(9314..9658)
                     /locus_tag="VGB68_15320"
     CDS             complement(9314..9658)
                     /locus_tag="VGB68_15320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050662.1"
                     /translation="MKVELLKLAHARSGDKGDTANVGVIALKDEFYPLLVREVTAEKV
                     KQHFGAMVKGEVERFELPNLNALNFLLHESLGGGGTLSLMTDAQGKTFSTALLRLKIE
                     ITETEANDLGVL"
     gene            complement(9655..10209)
                     /locus_tag="VGB68_15325"
     CDS             complement(9655..10209)
                     /locus_tag="VGB68_15325"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY0050663.1"
                     /translation="MNDKPETWKREASKQLADCRVFRVREDSSRRESDDLQHSFFVVE
                     SPDWVNIVALDAENRVVLIEQFRHGTEEIVLEIPGGMIDEGEAPDAAARRELLEETGF
                     ASDEFVFLGRSRPNPAIQNNWIYHFAALNCRKIQETAFDEHESVVTKMVSLDEIPQLI
                     ESEQITHSLVLAGFYKLNLHLKNL"
     gene            10282..11322
                     /locus_tag="VGB68_15330"
     CDS             10282..11322
                     /locus_tag="VGB68_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050664.1"
                     /translation="MNGKLFFIAILTIFISTFAFAQRTVTKKTPSPTPTPMSPVSPVP
                     TPSPTPVTLEIILTEAEKQTQNYRQAFNSLLAEETKTFEEYNRDGEVKKRRTVESNFL
                     VYQASKNENWIVEYRNVVKVDGKSVGDSNKRAEDFFEDVLKSTSAEAELQKILAESTR
                     YDKTLNISGFTLNQSPILAAHIRPALDFRLSGQETLNGRKVYAITYLQKAKSPYLLFN
                     ERPANTEKLYINYEVELPKQIKEPNASLRGKLWIDAETFEVWREEREQTIQIDNNPAV
                     VFRTEFEYQKNEPGFVTPKKIILTYYDVRRKDEQFIVVKDTVATFEYTKFTKSDVEVN
                     SGDVKSSETPKP"
     gene            complement(11678..>12460)
                     /locus_tag="VGB68_15335"
     CDS             complement(11678..>12460)
                     /locus_tag="VGB68_15335"
                     /inference="COORDINATES: protein motif:HMM:NF018938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEY0050665.1"
                     /translation="EDWDKIAAGTIAGHIIECGAQCSGGNCQYDWQNIPDLANVGFPI
                     VEAFPDGEFVVTKHEGTGGIVNIPSIKEQLLYEMGDPKSYITPDVVADFTSINLKTDG
                     ENRVRVHGIKGNPNTEFYKVSIAYAAGWKAVGTLVYAYPDAYAKAQAADKILRARLER
                     LGLKFDVILTEFVGVNATHGHLAGEPPKDIPEVQLRFGVRGQNKADVERFTKELAPLI
                     LTGPPAVTGFAGGRPKVEEIMAYFPALIPKTLIETKVEVITA"
BASE COUNT         2963 a         3164 c         3059 g         3274 t
ORIGIN      
        1 aataaaattt taaaaatgaa atttacgatt gaggaatttt tagaaaagct gcctttgccg
       61 gcgaatgaaa aatggaaaga cggcgtttgg gacgtcgagc cgtttaaaaa aggaaatgtc
      121 acgctcgttt ttttcgcgcc gcgcgcagcc gattatcaaa cttttcacga agatgacgaa
      181 ttttatttta tcgcgcgcgg aaatgccgag ctaatcgtcg aaaacgagcg ttttgcctgc
      241 gagacgggcg actgtttttt cgttccggcg caaaagaaac atcatttcga gaatatgtct
      301 gacgagtttg cgacgtgggc gatatttttt aagtgagaac tgataaatga taactgagaa
      361 tggaaacatc gccatttatt cagctctcat cacacttccg atagttttgc acgcaattcc
      421 acataatcct caatcgccgc gccgattaca tcgtaagatt cctcgcgccc gtagatataa
      481 gcgatgtcga tgatgctttt ctcgctcggc agagttttgt aggtcagaaa ataatgctga
      541 agacgattga cgatgctttt gggcagttcg gaaaggtctt tgtagttttc gtaaatcttg
      601 tcgccgacca gcgtggcgat aattttatcg tccgcctcgc cgttgtcgat caggcaaaat
      661 ccgccgatcg gcgtcgcttt cagaataatg tcgccgcgcg gaatgtggtg ctccgagagc
      721 accagaatat cgagcgggtc gccgtcgcct aacgtaattt ccttgccgga tttttctttt
      781 gccagccgcg ccacgcgctg cccgcaataa gtttgcggaa caaaaccgta attggcggga
      841 acgacatttg aatactgctg cgggcggtcg attttcagat aacccgtttc cttgtcgatt
      901 tcatatttga ccgtatcgcg cggaacgatt tcgataaaaa ccgtgacttc attcggcact
      961 ttatcgccaa tcgagatgcc gtgccaggga tgtgctttgt tgagtcttaa cattattaaa
     1021 ccttgcttgc agttacaatg tgttatgcgt cgttaccgta tgaaaacgaa gcgattcgga
     1081 tgattcggca aaactccgta aaaacaggta acaggtacat tttccgctac agtcttcaga
     1141 aaatgcaaat ccgtttcgta tttgccagcc aatcgaatca cgcgctgaat ttgtatttgg
     1201 cagccaatcg attcacgcac tggaattgat ttccccggct tcagaaaact cgttttaacc
     1261 gcgaattcga cctttcggca aaactaaaat ttgatttttt ataaagtatt gtatttatta
     1321 tcatttagat atacaaaaaa cttgtataac gtgtaaatca gtgacttcat tttctctcct
     1381 tatttgcgtt aaaattttta ccgtaaatat gagccaatca atcattgtcg aaaaaagcgg
     1441 gaaagcggcg attattagat tcaatcgtcc cgcaataaaa aatccgctct ccgtcgaaac
     1501 actggaaaat ctcgaacaaa tttttacgtt attgaatttt gatacgacag ttaaaaaaat
     1561 tatttttacg ggttcgggcg atacgtttgc ttcgggcgca aatctgaacg aggttgccgc
     1621 attgacaacg gaaacggcgc gggagttcgg cttgcgcggg caaaatctga tgcggaaaat
     1681 ataccgggcg gaaaaaataa cctttgccgc catcgacggg ttttgtatgg gcggcgcgct
     1741 ggatttggct ttatcctgcc gtcggcgcgt cgcttcgccg cgctcgattt tcgcgcatcc
     1801 cggcgcgagc ctgggcatca tcaccggttg gagcggaaca cagattctgc cgcgtctggt
     1861 cggtaagaaa aaggcgttcg agatattttt gacggcgaaa aaagtttcgg cgcaggaagc
     1921 tctggaaatc ggtttgattg ataaaatttc ggctgaccct ttgcaatttg cgacggcttg
     1981 agcagacgcc ttattttatc tgccgtccgc gccgaccgtc gcctcttttt tagactttct
     2041 taaatcgccc gtttcctcga ccgaatcgaa tttctcttcg ccggtcttta acttatcgtt
     2101 tctctcgacc gtgatttttt cgtttttctc gtcgcgcgag accggaatgc cgttgattga
     2161 tttgtattgt tcgacgccgc gattgttttg cgtagtttcg gtctcagttc gaatcgtctg
     2221 gtaattttcc ggcgcgattg tttcattctc ctgaaattga gcatacagcg cgtcgggctt
     2281 tggctggtgg cgcaggcatt ccaggtttga aaaggatttt gacggcacga cgactttttc
     2341 gcgctgcggg caaaaatcgt cggcgagcat tccggtttcg gggtcaatcg tcacttcaac
     2401 cagccctttc ggcatcggaa acgcgctgcc gccgaattcc gggcgcgcct cggttgtttt
     2461 tctcataaaa tcgacccaga ccggaagcgc gacttcgccg ccggtaaagc cgatgtcctt
     2521 gttttcgtcg aagcctatcc agacgacgca aaccagattc ggcgtgtagc cgacaaacca
     2581 gccgtctttc gagctgccgg ttttgccgac aaaggcgacg tcttttccca gagagtcttt
     2641 tgccgactgc gccgtgccgc gctcgacgac cgcgccgagc atatcggtta tcatatacgc
     2701 cgtctgcggg ctgataatct gcgtgtccgc atttttgttt ttataaaccg gattgccttc
     2761 gccggagagg atttgcgaaa cgaaaaccgg attgacgcgt ttcccgccgc ccgcgaaagc
     2821 ggcgtaagcg gcggcgagtt cgagcggcgt gacttcttcc gttccgagag ccatcgaagg
     2881 ataagcctcc ggtcttttca ttccgaattt ttcggcggtt ccggcgactt tctgcaaacc
     2941 gctttcgagc gcgatttcga ccgccgcgac attcgaagat tttgccagcg cggttttcag
     3001 agtaatctcg tgcatcgcgt aggaatttcc gtaatttgcc ggtttataag gcttgcggtc
     3061 gatggcgaaa gcggtcggct ggtcgtatat cagattcgcc ggcaattttc cctgttccag
     3121 cgcggttgcg taaacaaacg gcttaaaggt cgaaccgggc tggcgacgcg cttcggtcgc
     3181 gcgatttagc tgcgatgcgg cataatcgcg cccgccgacc attgccagaa catgcccgtt
     3241 tttcgggtca atggcgacga gcgcagcttg gggagtaacg ccttttttgg caaaagtctt
     3301 atcgagttcg gcgagtttct tgttaacgga gtcttcagcc atctcctgca attgcgggtc
     3361 aatcgtcgtg taaacgcgca gatttctttc aggcagtttt tcgccggaaa tttctttgtg
     3421 aaccgcgtcg acaaaatacg gcgcaatcga cttgtcgttt ttcgccggtg cggctaaaac
     3481 gatttcttcc gcctgagctt tttccgcctc ctgcggcgaa atcaatcctt cttcggataa
     3541 tttttggata accagattgc ggcgttccct ggcggcttca taattacgat tcggcgcgta
     3601 tttatgcggg ctttttatca ttgccgccag cgtcgccgct tccgccaggc taagattttc
     3661 tatccgtttg ccgaaatagc tgcggctcgc ctgctcgacg ccgtgaatgc cgatcgagcc
     3721 gtgctgtccg aaataaatct cgttgcagta aagcgcgaaa atgtcttctt ttgacagatt
     3781 tttttccagc gccagcgcca gaaaagcttc ggaaaatttg cgtccgaaag ttttttcatt
     3841 gctgagaaaa acgtttttga cgaattgctg cgtgatggtc gagccgccct gactgatttg
     3901 attgtcggag aggttgcggg aaaaagcgcg aaggatgccg cgcgggtcga gcccgccgtg
     3961 ttcaaaaaaa cgccggtctt cggtgtagat aatcgcgttt cgcaatagtt cgggaatctg
     4021 ttcgaatttc agtgtgttga ttgaatcgcg cttggcttcg gagtttccgg aaatcatttc
     4081 cggctgcaat tcgtaggttt ccagtgcgtg atttccgttt ttgattttcg agattttgcc
     4141 tttcgaaatt tccagattga taatttccgg cacgtcgcct gcggaatgat tggtctgaat
     4201 cgagacggcg tcgcctttga cagtaaaatt gccgttccag atgtttctgt tttcgccttc
     4261 gacatagccg gaatgacgca aaatggcgac gatttcttcg gttttgattt tctgcccggt
     4321 ttttaaaacg tgcggcgcgg cgtaaattcc cgagcgcgtc tgccagaaac cgcgtttcat
     4381 tcgctggtcg atgatcatcg ccgcctgcga ataaaaataa taaaagccga ttgccgccag
     4441 cgccgagcag aaaagaagcg tcaggctgat actgaaaatc cattttttaa gagcgctctt
     4501 tcggttgtta acaattttag cggcgggcgc agtttccgtg actgcggttt ttttgccttt
     4561 aggaaaactt ttgacgctta aagatttatc cagcttaatt attgccgtcg ccatattttt
     4621 gcccaccctt gctcgaaatc gattatgctc gaagctatgg caatgcgcgt tccacaatga
     4681 aaataatatt cagacgggat tgcaaactga tgaaatcaaa taaattaagc cggcagggaa
     4741 gtctttatca aggcagagtt tcattaaaat aaaaagaccc gaagcgtaaa tttgcgatac
     4801 tgaaatttgt atcaagttaa tacaacatta tttatctttg tttaaaaacc tggaaatccc
     4861 gttctggcaa tcgtcggtga ggcgggcaat cgtgttgacg tcgacgccgg attcgagcgc
     4921 cgattcaaac gtcaaaccgt ccatttgata aagcaggcgc ttcgacaaaa cgattgccga
     4981 gcgcgagact ttttcgtaaa ttgaaacgta attttttact tcttccgcaa aaaccgaatc
     5041 ttcaaaaatc cgatttacca gaccgaattc gcgggcggtt tccgcgccga attcaaagcc
     5101 ctgcgtcgcc agctcaaaag cgcgtttttc cgaaacgttg cgccttagaa tcgccatcac
     5161 catcgccgga acaaaaccga ttttgacttc cggatagccg aatcgcgccg acgcgcctgc
     5221 cagaaccaaa tcgcaagccg tcgccagacc gcagccgccc gccagagcgc gaccgtgaac
     5281 cgccgcgaca accggaattt tcacctttct aatcaaagcg aaaagctcca tcagattgtg
     5341 cgcgtcatcc caattttcca aaacattgct ttccgaaatt ttttgcagag cagataaatc
     5401 cgcgcccgaa caaaaatcat tgcccgcgcc tttgataaca atcgcgcgca gttcccggtt
     5461 tccgtctgcg tcgcgcagcg cgtctttgag cgagtttacc agcgcgtcgt taagggcgtt
     5521 tcgcttttcg gggcgattta aagtcagata acaaatcgag ttttcctgat tgattaaaat
     5581 ttcactcatt ttctttcgac gttttaacga ttgaagtcaa taatttaaaa atctcggcgc
     5641 ggtcggtctc aatcgaagca aattccttgt cgctcaaata atcgccctga tgcagaagct
     5701 gaagccaata ttcggcttcc gacgccttcc ggcgggcaac gcccatttcg tgaacgaaac
     5761 tctggcgcga ttccgcgttg acggcttctt tgatgtgttt cccgatatgc gttcccgaaa
     5821 ttaacaactc cttgcttaat acgtattctt tcttttcttc aactaaataa cggtaaagcc
     5881 tgactattct taaagcaaag acgagagatt tttctaaaac cggattatct ttcataaatt
     5941 attaattcta aattctaaat tcattcgcat acaaataaaa catattgtcc aggataattt
     6001 agaattaata atttagaatt cagaattgga tttagcgaat caaattcgtc gctttatcaa
     6061 tttccgaagc gaaattttcg attgttcgag ccgcgatttc gatttgctcg ctcgcttctt
     6121 tccagtcgcg ctgctcgatt gcttcacgga cgccgggcag agttttcacg ccgtagccgg
     6181 tgtaaaatcc gggcgcgtaa atttggtgct tgaaccacga gcggcgcggc aagccttcgc
     6241 tgcgcgtcaa tgcgcgttcg gcgcgataca gaatttcgcc cagagattta tgcgcggcgg
     6301 gcgacaattg tttgtttttc gatgccgcct cgtatttttt cgcgctttcc tgaagctttg
     6361 ccagcgcatt ctggagcggc gcaaaattca aaaacggcac gtccgctttc ggtttcggcg
     6421 cgacgaaagt ttccgccggg tcctggacga ccgcaaacat tctgtcggaa atcattcggt
     6481 tcgtcatcct ggtttcttcc cgcagattgt cggcgagcgt cgttacctct ctgacgtaag
     6541 tcgaaatcgt gtcggcaaaa ttcgtaaatt caaacggcag aatctcggcg ttggcgaagc
     6601 gcagggttgt ccgcccgcaa actttcgcca gcgcgatgcc gtaatcgaaa ttcgggtcgc
     6661 cgaagcgcgt aaaatggtcg aaagaatcgt aaaccgaatg atacgagcca ccgccgtctt
     6721 cgccgccgaa accgatatta aaggaagcga tgccgagatg ctgcaaaaac ggcgtgtagt
     6781 ccgagccgga gccgagcgcg taaatccgta aatcgccgcg ctccaacgct tcgcggcgcg
     6841 aattgggcga gccgttgacc gcttgcagcg ctttcaaacg ctgccagacg ctgattttcg
     6901 tctgcgggtc gataacgttt ttagcgactt cattggcgaa tttttcgagt gtgtgcgagc
     6961 cgccgacgcc gagaaaaccg cgtccgttcg aatcggaatt gatgtaaacg acggcttttt
     7021 gccgcagctc gtcggcgtga agctccgccc attcggtcga gcctaaaagt ccgggctcct
     7081 cggcgtccca agccgcgtaa ataatcgtcc gcttcggctt ccagcctgtt tttgccagct
     7141 cgccgacggc gcgcgcttct tccatcagcg cgaccattcc gctaatcggg tcgtcagcgc
     7201 cgttgaccca cgcgtcgtgg tgattgccgc gaataatcca ttggtcggga aattccgcgc
     7261 cattcatttt ggcgatgacg ttgtaagcgg gcttgatgtc ccagttaaat tccagcttca
     7321 gacgaacttt caacgaattt gcgccgccga aatgatatgt aatcggcagc gcgccgcgcc
     7381 agctctgcgg gacgaccgcg ccgcccaggt ttcttaataa aggcagggcg tcgccgtagg
     7441 aaatcggcaa aacgggaatt ttcgtcagcg tcggcgcgtc ttttctgtcg agccgtttcg
     7501 cgtctttggt cgagccgacg cccggcgtca gcgggtcgcc cggaaaaagc ggcatatcgg
     7561 cgaccgagcc gcgctgcgcg ccgttttcgt tgcgatacgc gcctttcggg taaacgtcgc
     7621 cctgaaaata accgtcgtcg cgcgggtcgg aataaatcag acagccgatg gcgccttttt
     7681 cggcggcgac tttcggcttg atgccgcgcc acgagccgcc gtagcgggcg atgacgattt
     7741 ttcctttcac gtcgatgccg cgtttttcca gttcttcgta atcggcggga atcccgtaat
     7801 tgacatagac gaggtcgccg ctgacgtcgc cgtcaatcga ataagcgttg taaatcggca
     7861 gctgctcgct ttgctggttt gaagtcgcgt cttcttttaa ttccggctcg attaattttg
     7921 ccgtaaattt ttcgggcgcg gtcatttcca gaagccgcgt tttcggcgtc ggaaacaaaa
     7981 cgtcgaactg ctcaatccgc gtctcgaaac cccacgattt aaacagcgca gccataaatt
     8041 cggcgttttg ttttccgtaa ggcgaaccga cgtgatgcgg gcgcgccgac agccgcttca
     8101 tccaatcgcg cagattctcg cgtcttagat acgaatcgaa tttttcttcc agagccgttt
     8161 gtcgagcgga gcccgcagcg gaaaagccga gcaaagattt ttcattcggc gtcgattgcg
     8221 cgacgacgga aaaagccagc agacagacaa agaaaactcg cagaaatgaa tttcgcatac
     8281 gcggactcct gaaatttagc ggatttggaa acgaagtgaa attattgtaa agaaaattcg
     8341 ggaaattagc cacacggaaa gaaacgatga atgatgaacg cggaacgatg aactgcaaat
     8401 tctgcgtttc attatttttt attcatcatt catcgttccg cgttcgtcgt ttattattat
     8461 tccgccagaa actcgcgcag aatttccgtc accgcttcgc tcgcttcctg ctgaacccag
     8521 tgcccgctgg tcggaatccg aaattcctca aacggcgcgt ccatcatctc gccgattccg
     8581 cgcacggttt ccggcaaaac cgctttatcc ttctcgccgt aaatgaaaac ggtgggaact
     8641 tttattttcg gcgtatcttc gagcttcgtg aaaaatcgct taacaatatt ggcgcggtaa
     8701 taattcagca tcgccgtcag cgcaaagggc tcgctccagg tctttttata tttggcgata
     8761 tcctcgtccg taaagacgtc cttttccgcc gtcgaatttt tcagagcgtt ttccagaagc
     8821 gcaaaatcct tgagctttaa caaattttcg ggcagcagcg ggatttggaa aaagaacata
     8881 taccagcttg ccgcaaactg cctgaacgtt tgatttttgc gccagacggc aaccggcgga
     8941 acttgcagcg cgccgagttt ccagagatat tcgggatgct tcgaagccaa agcccaagcc
     9001 acggtcgcgc cccaatcgtg cccgatgacc gccgctcttt cgcgcccgaa atgatgaatc
     9061 aagccgacga cgtcgtcagc cattttatcg atttcataat cggaaacgcc ggtcggctta
     9121 tcgctcagat tatagccgcg catatcgggc gcgacgaccg tgtattcgtc gcctaaagcc
     9181 gccagctgat gccgccacga ataccagaat tcgggaaatc cgtgcagtaa gataacgagc
     9241 cgctcgcctt cgcccgccgt tgcgtaatgc agccggacgc cgccgatttg cgcgtaatcg
     9301 aatgtaatct cgctcataaa actcctaaat catttgcttc ggtttcggtg atttcaattt
     9361 tcaaacgcag cagcgcggtc gaaaaagtct tgccctgcgc gtcggtcatc agggacaaag
     9421 ttccgccgcc gccgagagat tcgtgcaaaa ggaaattcaa ggcgttcaga ttcggcagct
     9481 caaaacgctc gacttcgcct ttaaccatcg cgccgaaatg ctgcttgacc ttttccgccg
     9541 tcacctcgcg aaccagaagc gggtaaaatt cgtctttcaa agcaatcacg ccgacgtttg
     9601 ccgtgtcgcc tttatcgccg gaacgcgcgt gcgccagttt taacagttca actttcataa
     9661 atttttcaaa tgcagattta gtttataaaa tccagccaaa acaagcgaat gcgtgatttg
     9721 ctcgctttcg attaattgcg gaatttcgtc gagcgaaacc attttcgtga cgacgctttc
     9781 gtgctcgtca aaagccgtct cctgaatttt ccggcagttt aaggcggcga aatgataaat
     9841 ccagttgttt tgaatcgccg gattcgggcg cgatcttccc agaaaaacga attcgtcgga
     9901 cgcaaagccg gtttcttcga gcagctcgcg ccgcgccgcc gcgtcgggcg cttcgccttc
     9961 gtcaatcatt ccgcccggaa tctctaaaac gatttcttcc gtgccgtgac gaaattgttc
    10021 gattaaaacg actcgatttt ccgcatccag cgcgacgata ttgacccaat ccgggctttc
    10081 gacgacgaaa aagctgtgct gtaaatcgtc cgattcgcgg cggctcgaat cctcgcggac
    10141 tcggaaaacg cggcagtcgg caagctgttt tgaagcttcg cgcttccagg tttcgggttt
    10201 gtcgttcata aaaatagtga cgcgattatt aaaaatatgt ttgaataata aaacaagttt
    10261 acattttttc agggggaatt tatgaacggg aaattatttt ttatcgcaat tttgacaatt
    10321 tttatttcga cctttgcctt cgcgcagcga accgttacaa aaaagacgcc gtcgccgacg
    10381 ccgactccga tgtcgccggt ttcgccggtt ccgacgcctt cgccgacgcc cgtcacgctg
    10441 gaaattattc tgaccgaagc cgaaaagcag acgcagaatt atcgacaggc tttcaacagc
    10501 cttctggcgg aagaaacgaa aactttcgag gagtacaatc gtgacggcga agtcaaaaaa
    10561 cgccgcacgg tcgaatcgaa ttttctcgtt tatcaagctt ccaaaaacga aaattggatt
    10621 gttgaatatc gcaacgtcgt gaaagttgac ggcaaatcgg tcggcgacag caacaagcgc
    10681 gccgaagatt ttttcgagga cgttctgaaa tcgacgagcg ccgaggcgga actccaaaaa
    10741 attctcgcgg aaagcacgcg ttacgacaaa accctgaaca tttccggctt tacgcttaat
    10801 caatcgccga ttctcgccgc gcatattcgt cccgcgctgg actttcgcct gagcggtcag
    10861 gaaactttaa acggcagaaa agtttacgct ataacttacc tgcagaaagc aaaaagcccg
    10921 tatcttttat ttaacgagcg cccggcaaat accgaaaagc tttatatcaa ttacgaggtg
    10981 gaattgccga aacagataaa agagccgaac gcctctttac gcggaaaatt gtggattgac
    11041 gccgaaacgt ttgaagtctg gcgtgaagaa cgcgagcaga caattcaaat cgacaataat
    11101 ccggcggttg ttttcagaac cgaattcgaa taccagaaaa acgagcctgg ctttgtcacg
    11161 ccgaaaaaaa tcatcctgac ctattacgat gtcagacgta aggacgaaca gtttatcgtg
    11221 gtcaaagaca ctgtagcgac gtttgaatac acgaaattca cgaaatccga cgtcgaagta
    11281 aactccggcg acgttaaatc gtcggaaacg ccgaagcctt aaagactttg ttcgccggca
    11341 cgtgaaaatc gcatccggcg cgtgaaagat tgatatttca cgcaaaagat tgaaatttcg
    11401 agtgaaagta caccattgcg tgcaaaagta tcatattgcg gatatttgag cgttattgcg
    11461 gacggttgat tcatcttgcg gctgaatgac taatcttgca gacgtttgaa tgatattgcg
    11521 ggcgttcgat tcatcctgca ggtaaaaggc tgttaacaaa acggtttctg cgttggaatg
    11581 aaacttaaga gagcggtaaa cggtaaacgg taagcggtaa gttgcagtta taaaataacg
    11641 agcttaccgt ttaccgttta ccgtttattc ctgagtctta cgccgtaatt acttcgactt
    11701 tcgtctcgat cagagttttc ggaatcagcg ccggaaaata agccataatt tcttcgactt
    11761 tcggtctgcc gcccgcgaaa cccgttacgg cgggcggtcc ggtcaggatt aaaggcgcca
    11821 gctctttggt aaatctttcg acatcagctt tattttgccc gcgcacgccg aagcgcagct
    11881 ggacttcggg aatatctttc ggcggctcgc ccgccagatg cccgtgcgtg gcgttgacgc
    11941 cgacgaattc cgttaagatt acatcgaatt ttaaaccgag tcgttcgagc cgcgcgcgca
    12001 ggattttgtc ggcggcttgc gccttcgcgt atgcgtccgg gtaagcgtaa acgagcgttc
    12061 cgacggcttt ccagcccgcc gcgtaagcga tggaaacctt gtaaaattcc gtgttcgggt
    12121 tgcctttgat gccgtgtact cgcacccggt tttcgccgtc cgttttcaaa ttaatcgaag
    12181 tgaaatcggc gacgacatcc ggcgtgatat aagatttcgg gtcgcccatt tcatacaaaa
    12241 gctgttcttt tatcgaagga atattgacga ttccgcctgt tccttcgtgt ttcgtgacga
    12301 cgaattctcc atccggaaaa gcttcgacaa tcgggaagcc gacatttgcc aaatcgggaa
    12361 tattctgcca gtcgtactgg cagtttccgc ccgagcattg cgcgccgcat tcgatgatat
    12421 gaccggcaat cgtgcccgcc gctatcttgt cccagtcttc
//
LOCUS       DASWNE010000789         5164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_485528, whole genome shotgun sequence.
ACCESSION   DASWNE010000789 DASWNE010000000
VERSION     DASWNE010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485528"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1264
                     /gene="zwf"
                     /locus_tag="VGB68_15340"
     CDS             <1..1264
                     /gene="zwf"
                     /locus_tag="VGB68_15340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEY0050666.1"
                     /translation="KERTYYTGGDFDDDKKLFRDLKDLLAEVCESHQIPENFFYYLAT
                     PPSLFANVTGKIHKNGLAQEKNGNWRRFIYEKPFGHDLESAETLNAELLKILDERQIY
                     RIDHYLGKETVQNILVFRFGNSIFEPIWNRNYIDHVQITVAEQLGVESRGNYYETAGA
                     LRDMVPNHIFQLITLTAMEPPVSFEADAVRDEQAKILQAIQPFTEEDVLRKAVRGQYD
                     AGEMNRKEVAAYRSEPNVAPESNTETYAALKLSIDNWRWADVPFYVRTGKRLPARHSD
                     IVIQFKKAPFILFRDTSIERLTTNRIVIHIQPDEGITLHFGAKIPGPIVNMGAVDMDF
                     NYLEHFSETGSTGYERLLFDCMIGDATLFQRADMVEASWQIVSPILDIWRAIPARSFP
                     NYQAGTWGPKEADELLAHDGRSWRNVGT"
     gene            complement(1416..1841)
                     /locus_tag="VGB68_15345"
     CDS             complement(1416..1841)
                     /locus_tag="VGB68_15345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050667.1"
                     /translation="MDINISDFLTPRNVMLAILVLPFLTMLVFVPFLYTISRNAREWL
                     TIFGFLGFIANIVLAVECCYRILNSILAYATQKPTLVFDQKSSRMVYQQEPYDFRYIG
                     YALIFFGISFLTWQLLNKFSPWETKRNKESEVISLDLNK"
     gene            complement(1891..3213)
                     /locus_tag="VGB68_15350"
     CDS             complement(1891..3213)
                     /locus_tag="VGB68_15350"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY0050668.1"
                     /translation="MRFVFSRRFYILLALGLIPLSLSWNLPALQTAVFAADALLVLAA
                     IIDYFVSRKLPEDFTVRREFSRRFAVGDETEARLIIENLSPHTFTVQIKDEFPSEMRL
                     VGEREARLKIEGQTFVRVSYGLIPPRRGRYEFGHTAVRFLSKLGLVWCQKNLNAAQAV
                     KVYPNMRRAREMELKALGARSFLAIQRKAVRRGEGREFESMRDYVRGDELRHISWTAT
                     ARRAKLTTRQYQIERDQTIIVALDAGRLMTGRIGDETKFDTAIHSSLALMSAAARGGD
                     NCGLMVFGRRIKKYLPPKKGVEHIDSVLEALHDLEPELIEPSYARAFQFIAANLKKRT
                     FVVILTDLVDKESSKELLNSLKLLRPRHLPLVVTIGDRDLNATVSRIPKELKEVFVQS
                     AAEEIIRQREAALRMVESLGGLALDVTTNSLAPRLLETYLRVKERGLL"
     gene            3417..3965
                     /locus_tag="VGB68_15355"
     CDS             3417..3965
                     /locus_tag="VGB68_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050669.1"
                     /translation="MKTVGELVQEAVDFMNAGSYEAAFVPTCAAINETAKKTFDAENY
                     QRFIRENWQLISFMGLPRALPLPLNIPFALKRIVPSFNSHHGAKEIIFHLVQQTLATG
                     KMPSSFAFASTDIFEIKKNKLLIPLSLLGGLLGVVIVQSVNRDEIIDEKYWMNIADFK
                     MFISELWGRIDLAERIMRFYLE"
     gene            4007..4720
                     /locus_tag="VGB68_15360"
     CDS             4007..4720
                     /locus_tag="VGB68_15360"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY0050670.1"
                     /translation="MRKIYYFLIAFAALPGVFYGLWELSKSRTFQAFGEIVPRVETSR
                     KLVALTFDDGPTPAHTDEILRVLKEENVEATFFLIGGDLEKHPSEGKKIVAAGHEIGN
                     HSYSHTRMVLVSASFVAGEIERTDALIRAAGYESPTHFRPPYGKKLFTLPYYLSQTGR
                     KSITWDVEPESFPKIAAKTDEIVKYILANTRAGSIILLHVMYDPERKSLNAVKPVIEG
                     LRGQGFQFVTVSELMAQSN"
     gene            4786..5106
                     /locus_tag="VGB68_15365"
     CDS             4786..5106
                     /locus_tag="VGB68_15365"
                     /inference="COORDINATES: protein motif:HMM:NF020908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HEY0050671.1"
                     /translation="MSLGKFIDEQIRNSIEAGDFDNLEGAGRPIDLDSYFNTPEDLRM
                     AYSVLKSSKFVPEEVERLKEIGELKEKVKSCADDEERKKINKILQERQLAFSLLMERN
                     KRRR"
BASE COUNT         1376 a         1279 c         1291 g         1218 t
ORIGIN      
        1 taaggaaaga acctattaca cgggcggcga ttttgacgac gataaaaaat tgttcaggga
       61 tttaaaggat ttgctggcgg aagtctgcga aagccatcag attccggaaa acttttttta
      121 ctatctcgcc acgccgccgt cgcttttcgc caacgtcacc ggcaaaattc ataaaaacgg
      181 tctggcgcag gaaaaaaacg gcaactggcg acgctttatt tacgaaaaac ctttcgggca
      241 cgacctcgaa tccgccgaaa ctttaaacgc cgagctttta aaaatcctcg acgagcggca
      301 gatttaccgc atcgaccatt atctgggcaa agaaaccgtc cagaatattc tggtttttcg
      361 cttcggcaac agcattttcg agccgatttg gaatcgaaac tacatcgacc acgtccagat
      421 tacggtcgcc gaacagctcg gcgtcgaatc gcgcggaaat tattacgaaa cggcgggcgc
      481 gctgcgcgat atggttccga atcacatttt ccagctcatc accctgaccg cgatggagcc
      541 gcccgtctcg tttgaagccg acgccgtgcg cgacgagcag gcgaaaattt tgcaggcgat
      601 tcagcctttc accgaagaag acgttttgcg taaagccgtt cgcgggcaat acgacgccgg
      661 cgagatgaac agaaaagaag tcgccgctta tcgaagcgag ccgaacgtcg cgcccgaatc
      721 gaacacggaa acctacgccg cgctgaagct ctcgattgac aactggcgct gggctgacgt
      781 tccgttctac gtccgcaccg gcaaacgcct gcccgcgcgt cattccgaca tcgtcattca
      841 atttaaaaaa gcgccgttca tccttttccg cgacacttcg attgagcgcc tgacgaccaa
      901 tcgaatcgtc attcacatcc agccggacga aggcataacc ttgcatttcg gcgcgaaaat
      961 tccgggtccg attgtcaata tgggcgcggt cgatatggat ttcaattatc tggagcactt
     1021 cagcgaaacc ggcagcaccg gctacgaacg ccttctcttc gactgtatga ttggcgacgc
     1081 cactctgttt cagcgcgccg atatggtcga ggctagctgg caaatcgtct cgcccattct
     1141 cgacatctgg cgcgccattc cggcacgctc tttcccgaat tatcaagccg gaacgtgggg
     1201 accgaaagaa gccgacgaat tactggcgca cgacggcaga agctggcgaa atgtggggac
     1261 ttaacggttg aatcgacgcg ggcgcaatcg acattgaatc aacaaaattc gggatttcgt
     1321 tatggatatt ccggggattg gataatttaa tttctgattt ttacgatgta taaactttaa
     1381 acggaacgat tatttgcaga accttaccgc cggggttatt tattcaaatc aagcgaaata
     1441 acttccgact ctttatttct ctttgtttcc cacggagaaa atttattgag taattgccag
     1501 gtcaaaaatg agatgccgaa aaatatcaat gcatagccaa tgtatctaaa gtcgtaaggc
     1561 tcctgctgat aaaccatccg gctcgacttt tggtcaaaaa ctaatgtcgg tttttgtgtg
     1621 gcgtaagcta aaattgaatt caggattcga tagcagcact cgacggcgag cacgatattt
     1681 gcaataaatc ccaaaaaacc gaagatagtc agccattccc tcgcatttct ggaaatcgtg
     1741 taaagaaaag gcacaaagac gagcattgtc aaaaacggca aaaccaggat tgccagcatc
     1801 acattccgtg gagttaaaaa atccgaaata tttatgtcca tctcaataaa gccataacaa
     1861 aatacaagga ataaaaagtc cggcagacgt ttaaagcaag cctctttcct tgactctcaa
     1921 ataagtttcc agaagacgcg gcgcgaggga attggtcgtc acgtcgagcg cgagcccgcc
     1981 gagagattcg accattctca aggcggcttc gcgctggcgg atgatttctt cagccgccga
     2041 ctggacgaaa acttctttta attcttttgg aatccggctg accgtcgcgt ttaaatcgcg
     2101 gtcgccgatg gtgacgacca aaggcagatg gcgcgggcgc agaagcttga gcgaattcag
     2161 gagctctttc gagctttcct tatcgactaa atcggtcaaa attacgacga aggtgcgttt
     2221 ttttaaattg gcggcgatga attgaaaagc tctggcgtag gacggctcaa tcaattccgg
     2281 ttcgaggtcg tgcagcgctt cgagaacgga gtcgatatgc tcgacgcctt ttttcggcgg
     2341 caggtatttt ttgatgcggc gtccgaaaac catcaagccg cagttgtcgc cgccgcgcgc
     2401 cgccgccgac attaaggcaa gactggaatg aatcgccgtg tcgaatttcg tctcgtcgcc
     2461 gatgcgaccg gtcatcaggc gtccggcgtc gagcgcgacg ataatcgtct ggtcgcgctc
     2521 gatttgatat tggcgcgtcg tcagctttgc gcgccgcgcc gtcgccgtcc acgaaatatg
     2581 ccgaagctcg tcgccgcgca cgtaatcgcg catcgactcg aactcacgac cttcgccgcg
     2641 ccggacggct ttgcgctgaa tcgccagaaa cgagcgcgcg ccgagcgctt tcaattccat
     2701 ttcgcgcgcg cgccgcatat tcgggtaaac cttgacggct tgcgccgcgt tcagattttt
     2761 ttgacaccag accagaccga gctttgataa aaaacggact gccgtgtgcc cgaattcgta
     2821 acgcccgcgc cgcggcggaa tcaaaccgta gctaacccga acgaaagttt gcccttcgat
     2881 tttgagtctt gcctcgcgct cgccgacgag ccgcatttcg gacggaaact cgtctttgat
     2941 ttgaacggta aacgtgtgag gcgaaagatt ttcgataatc aaacgcgctt ccgtctcgtc
     3001 gccgacggcg aaacgcctgg aaaactcgcg cctgacggtg aaatcttccg gcaatttgcg
     3061 actgacgaaa taatcgatta tcgccgccaa aacgagcagc gcatccgccg cgaaaacggc
     3121 tgtttgcagc gcgggcagat tccacgaaag cgacaacgga atcaagccga gcgccagcag
     3181 aatgtaaaat ctacgggaga agacaaagcg cataacttgc cgtcgattat acagaaaagc
     3241 ggcgcaaaga aataggacgc cgattaatca agacagtttg cgattattca ggattaactg
     3301 aaataaaatc ctaatccgtc ttattaaatc ctgaataatc gccagcgctc gctaaaattt
     3361 attttcataa atgcttttcg tttataatcg aaacagcggc gaggtgaaca gtagttatga
     3421 agacagtggg cgagctcgtg caggaagcgg tcgattttat gaacgcaggc tcgtatgaag
     3481 cggctttcgt gccgacctgc gcggcgatta acgaaacggc gaaaaagact ttcgacgcgg
     3541 aaaattacca gcggtttatc agggaaaact ggcagctgat ttcgtttatg ggcttgccgc
     3601 gagctttgcc gctgccgctc aatattccgt ttgcgctgaa aagaatcgtg ccgtccttta
     3661 actcgcatca cggcgcgaaa gagattattt ttcacctcgt tcagcagacg ctggcgacgg
     3721 gaaaaatgcc ttcgtcgttc gcctttgcct cgaccgatat ttttgaaatt aaaaaaaata
     3781 agcttctgat tccgcttagt ttgctcggcg gtctgctcgg cgtcgtgatt gttcagagcg
     3841 tcaaccggga cgagattata gacgagaaat actggatgaa cattgccgat tttaaaatgt
     3901 ttatctccga gctttggggc agaatcgatt tggctgagcg aattatgagg ttttatctcg
     3961 aataaaaatt tttgcgaaaa ggtgttttta aattaaaatt caaccgatgc gaaaaatcta
     4021 ttatttttta attgctttcg cggcgcttcc gggcgtcttt tacggactgt gggaattgag
     4081 caagtcgcgc acctttcagg ctttcggcga aatcgtgccg cgcgtcgaaa ccagccgaaa
     4141 actggtcgcg ctgactttcg acgacggacc gacgcccgcg cacaccgacg aaatactgcg
     4201 cgtcttaaag gaagaaaacg tcgaggcgac tttcttttta atcggcggcg atttggaaaa
     4261 acatccgtcc gaaggaaaaa agattgtcgc cgccgggcac gaaatcggca atcattctta
     4321 ttcgcacacg cggatggtgc tcgtctcggc ttcgttcgtc gcgggcgaaa tcgagcgaac
     4381 cgacgcgctg attcgcgccg ccggttacga aagcccgacg cattttcgcc cgccttacgg
     4441 caagaagctt tttacgctgc cttattatct ttcgcagacc ggcagaaaaa gcatcacgtg
     4501 ggatgtagag ccggaatctt ttcccaaaat tgccgcgaaa accgacgaga ttgtgaaata
     4561 tattcttgcc aacacgcgcg ccggctcaat catcttactg cacgtaatgt atgaccctga
     4621 gcgcaagtct ctgaacgccg tcaagcctgt catcgaaggt ttacgcggtc aaggttttca
     4681 gtttgtgacg gtttcggaat tgatggctca atcaaattaa tttaggttta atctcaaact
     4741 tttcaattta aaatttcgtt acagtattaa agagaaaaaa gttttatgtc cttaggaaaa
     4801 tttatcgacg aacaaatcag aaactctatt gaagcgggcg attttgataa cctcgaaggc
     4861 gcgggacgcc cgatagattt ggattcttac tttaacacgc cggaagattt gcgaatggcg
     4921 tactcggttt taaaaagcag caaattcgtg ccggaagaag tagaacgcct gaaggaaatc
     4981 ggcgaattga aagaaaaagt aaaaagttgc gccgacgacg aagaaagaaa aaaaatcaac
     5041 aaaattttgc aggaaagaca gctcgctttt tcgcttttga tggaaagaaa caagcgcaga
     5101 agataattgc gctcagacaa gaattctgcg gcggcatcgg gattttctga aattgcgggg
     5161 cgcg
//
LOCUS       DASWNE010000790         3357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_662742, whole genome shotgun sequence.
ACCESSION   DASWNE010000790 DASWNE010000000
VERSION     DASWNE010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662742"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2548
                     /gene="dnaE"
                     /locus_tag="VGB68_15370"
     CDS             <1..2548
                     /gene="dnaE"
                     /locus_tag="VGB68_15370"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY0050672.1"
                     /translation="KMKADGALKYSFGDYLERLNIEIDIIRRMGFPGYFLIVWDFIQY
                     AKSRDIPVGPGRGSAAGSLVAYCLEITDVDPLQYDLLFERFLNPERVSMPDIDIDFCV
                     RGRAEVINHVTEVYGRDSVCQIATFGTMASKAAIKDVGRALNMPYSDVEKIAKLIPPP
                     VRGRNISITQALEQVGELRQAMETDEKVKELVDLARRLEGCARHTSVHAAGVVISPKP
                     LHELVPTAISAKNELTSQYTMTDLEKVGMLKMDFLALTTLTIINDCLKSIKQRIGAEI
                     DWTTVSLSDEKTMALFGEGRTEAIFQFESSGMQEICRRLKPRELEDLAALNALYRPGP
                     LDGGMVDDFILRHRGEKRVSYIVPEMKDILSNTYGILVYQEQIMQLAQKLAGYTLGEA
                     DMMRRAMGKKKREEMAVHEEKFIQGAVARHIKREKAEEIFKLMAQFADYGFNRSHSVA
                     YALLAFQTAYLKAHYSAYFYAAVLSNESQDTAKIYKYSTELRHAGLQLLPPDVNESDS
                     GFTPTEDAVRFGLNAIKGIGSTSVQAILEARESGPFTSLYDFTARLEPGAINRRGLES
                     LITAGAFDSLKPPAQSINLWRACLFAAIDGSLSIAQKQWNDKIKGQNILFGAQETTNG
                     TIDVELPVVAAWSQHKISQEEKNAIGFYLSTHPLDDYKETLTHLKIKNIVEFQGLKPG
                     EKITMAGIVSGAQTRHSKKGNRFCIFKLEDQSTGAKCLAWSETFTKYAECLQENELLI
                     VNGKIESNEGQEMTVIVEDAQKLADALPTRAQKVLVTLPSTERFSEEFLDELFAMFSR
                     NRGSCDVYFNLELDKTISVNLLSQPIRISGTKFLENELLQKGCRVEWQFT"
     gene            2555..>3357
                     /locus_tag="VGB68_15375"
     CDS             2555..>3357
                     /locus_tag="VGB68_15375"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004344.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEY0050673.1"
                     /translation="MAKTAIERVATARHPERPYTLDLLASVFTDFVEIQGDRRFSNDP
                     AMICGFAKLGEVEVAVIGQQKGRDTNARKHRNFGMPKPEGYRKALRLMRLAEKFGRPV
                     ITFIDTPGAYPGIDAEERGQAEAIAFNLREMAGLKVPIIVLVIGEGGSGGALAIGVGD
                     RVLMMENAVYSVISPEGCAAILWKDASQAPRAADCLRLTAEDLKRLGVIDEIIPEPFE
                     WRIKDDADAESVDEVTENLKNSLLRHLKELLKFSAPQRLTRRYEKFRKMG"
BASE COUNT          851 a          872 c          901 g          733 t
ORIGIN      
        1 caaaatgaag gcggacggag ccttaaaata ttcttttggg gattatttgg agaggctgaa
       61 tattgaaata gatattatca gacgcatggg ttttcccggc tactttctaa tcgtctggga
      121 ctttattcaa tacgccaaga gcagagacat tccggtcggt cccgggcgcg gctccgccgc
      181 gggctcgctg gtcgcatact gtctggaaat tacggacgtc gacccgctcc agtatgattt
      241 gcttttcgag cgttttctga accccgaaag agtgtcgatg ccggatatcg atatcgactt
      301 ctgcgtgcgc gggcgcgccg aagtcataaa ccacgtgacg gaagtttacg ggcgcgattc
      361 ggtctgccag atcgccactt tcggaacgat ggcttcaaaa gcggcgatta aggatgtcgg
      421 gcgcgccttg aacatgccgt attcggatgt ggaaaaaatc gccaagctca taccgccgcc
      481 cgtgcgcggt cgcaatatca gcatcacgca ggcgctggaa caggtcggag agttaagaca
      541 ggcgatggaa accgacgaga aggtcaagga gttggtcgat ttggcgcgac gcctcgaagg
      601 ctgcgcgcga cacacatcgg ttcacgccgc cggtgtggtt atctcgccca aacctctgca
      661 cgaactcgtt ccgacagcca tctccgccaa aaacgagctg accagccaat acaccatgac
      721 tgacctggaa aaagtcggca tgcttaagat ggattttctt gctttaacta ctttaacaat
      781 cattaacgat tgcttaaaaa gtattaagca gagaatcggc gccgaaatcg actggacgac
      841 cgtttctctg agcgacgaaa aaacgatggc tctgttcggg gaaggcagaa ccgaggctat
      901 attccaattc gaatcctccg gcatgcagga aatttgccgg cgtttaaaac ccagagagct
      961 ggaagattta gccgccctga acgcgctcta tcgccccgga ccgctcgacg gcggaatggt
     1021 cgacgacttt atccttcgtc atcgcggcga aaagcgcgtc agctatattg ttccggaaat
     1081 gaaagacatt ctttcaaaca cttacggcat tctcgtctac caggaacaga tcatgcagct
     1141 ggcgcagaaa ctggctggct acacgctcgg cgaagccgat atgatgcggc gcgcgatggg
     1201 caaaaagaag cgcgaggaaa tggctgtcca cgaggaaaaa tttattcagg gcgccgtcgc
     1261 tcgtcacatc aaacgcgaaa aagccgagga aatctttaaa ctgatggcgc agttcgccga
     1321 ctacggattt aaccgcagtc acagcgtcgc ttatgcttta ttggcgtttc aaactgctta
     1381 tcttaaagcg cattattccg cttactttta tgcagccgtt ttatcgaacg aatcccagga
     1441 caccgccaaa atctacaaat actcgaccga gctgcgacac gccggactgc agctcttgcc
     1501 gccggacgtc aatgaaagcg attcgggatt tacgcccacc gaagacgccg ttcgtttcgg
     1561 gctgaacgcc attaagggaa tcggctcgac gagcgtgcag gcgattttag aagcgcggga
     1621 aagcggtccc tttacttccc tgtacgactt tacggcgaga ctcgagcccg gagccatcaa
     1681 ccggcgcggt ctggagagcc tgattaccgc cggcgccttc gattcgctta agccgcccgc
     1741 gcaatcaatc aatctctggc gcgcctgcct gtttgccgcc atcgacggtt ccctgtcgat
     1801 agctcagaaa cagtggaatg acaagattaa agggcagaac attcttttcg gcgcgcagga
     1861 gacgacaaac ggcacgatcg acgtcgaatt gcccgtcgtg gcggcgtggt cgcagcataa
     1921 gatttcgcag gaagaaaaaa acgccatcgg cttttatctt tccactcacc cgctggacga
     1981 ttacaaggaa actctgacgc atctgaaaat caaaaatatc gtggaatttc aagggcttaa
     2041 acccggagaa aaaattacga tggcgggcat cgtctccggc gcgcaaaccc gtcacagtaa
     2101 aaaaggaaac cgcttctgca ttttcaagct cgaagaccaa tcgaccggcg ccaaatgtct
     2161 cgcctggtcc gaaactttca caaaatatgc ggaatgcctc caggaaaacg aacttttgat
     2221 tgttaatggt aaaatcgaat cgaacgaggg acaggaaatg accgtcatcg tcgaagatgc
     2281 gcagaaactg gcggacgcgc tgccgacgcg ggcgcagaag gttctggtga ctctgccttc
     2341 gaccgagcgc ttttccgaag aatttttgga cgagttgttc gccatgttca gtcgaaatcg
     2401 cggctcatgt gatgtatatt tcaatctgga actggacaaa accatctccg tcaatctgct
     2461 ttcgcagccg atacgaattt ccggcacaaa gtttctggaa aatgagctgc tgcaaaaagg
     2521 atgccgggtc gaatggcagt ttacataaat ttttatggct aaaacagcaa tagagagggt
     2581 cgccacggcg agacatcccg aacgaccgta tactttggat ttacttgcca gcgtctttac
     2641 ggattttgtc gaaattcagg gcgaccggcg cttttccaat gacccggcga tgatttgcgg
     2701 atttgccaaa ctgggcgagg tcgaggtcgc cgttatcggg cagcaaaagg gacgcgatac
     2761 aaacgcacgg aaacacagaa atttcggtat gccgaagccg gaaggctacc gcaaggctct
     2821 ccgtctgatg cgcctggcgg agaaattcgg cagacccgtt atcactttta tcgatacgcc
     2881 cggcgcttat ccgggcatcg atgccgaaga acgcgggcaa gccgaagcca tcgctttcaa
     2941 tctgcgtgaa atggctggtc tgaaggttcc gattatcgtg cttgtcatcg gcgaaggcgg
     3001 ctccggcggc gcgctggcga ttggcgtcgg cgaccgtgtc ttaatgatgg aaaatgccgt
     3061 ctattccgta atttcgcccg aaggctgtgc ggcgattctc tggaaagacg cttcacaggc
     3121 tccgcgcgcg gctgattgcc tccgtctgac cgccgaggat ttaaagcggc tcggagttat
     3181 cgacgagatt attcccgagc cgttcgaatg gcgaataaag gacgacgcgg acgccgaatc
     3241 ggtcgatgaa gtgaccgaaa accttaagaa ttctcttttg agacacctga aagagctttt
     3301 aaaattttcc gctccgcaac gtctgacccg gcgctacgaa aaatttagaa aaatggg
//
LOCUS       DASWNE010000791         1896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_274647, whole genome shotgun sequence.
ACCESSION   DASWNE010000791 DASWNE010000000
VERSION     DASWNE010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274647"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VGB68_15380"
     CDS             complement(<1..334)
                     /locus_tag="VGB68_15380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050674.1"
                     /translation="MNEAFFKNNLITLAKIGQKLADSAGMRDSLGKTLATIHESTLFV
                     RSFAALYESQTEKLSLYAAEGLNVNEYRRLENRLNKGFLGGEANGEKRILVERAAMEV
                     SFDFVSPRE"
     gene            751..1677
                     /locus_tag="VGB68_15385"
     CDS             751..1677
                     /locus_tag="VGB68_15385"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY0050675.1"
                     /translation="MDETLHFQVDKSSHKLRLDEFLFDKIRGVSKMYLRDLIKTEKCL
                     VNGTVENRGYKLRTNDFVEIEMDRRVERAMKPEPLPLEIIFEDAEIIVVNKAAGMLMH
                     PTFAQKTGTLLNALAFYLNFSAHSCEDSEKNTKDKGQGKGDESKFIRPGLIHRLDRQT
                     SGLLVVAKTPRAHRVLSDHFRRKLVEKRYFALVEGVVGEDSGTILAPIGRIEEIRLWN
                     VKEDGKHAETRFRVKERFSDSTLLELEPVTGRTNQLRIHCAHIGHPIIGDEWHSRREF
                     PRLCLHAYKLAFWHPDGNRWMEFEPARENYFA"
     gene            complement(1702..>1896)
                     /locus_tag="VGB68_15390"
     CDS             complement(1702..>1896)
                     /locus_tag="VGB68_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050676.1"
                     /translation="GGDERAVRQTFHPQMLSYFDQLPTVNIDGGGNFIFVYESNKTAP
                     SENLNWFIGERIKYLNLFGS"
BASE COUNT          548 a          457 c          433 g          458 t
ORIGIN      
        1 cttcgcgcgg agaaacaaaa tcgaaagaaa cttccattgc ggcgcgctcg accaggatgc
       61 gtttttcgcc gttcgcctcg ccgcccaaaa aacctttatt caaacgattt tccagccgcc
      121 gatattcgtt gacgttgagt ccctccgcgg cgtagagcga aagtttttcg gtttgcgact
      181 cgtacaaagc ggcaaagctc cggacgaata aagtcgattc gtgaatcgtc gccagagttt
      241 tgccgaggct gtcgcgcatt ccggcggaat cggcgagctt ttgcccgatt ttagccaatg
      301 ttataagatt gtttttaaag aaagcttcgt tcatttccta caaaattgta ttacaaataa
      361 aattacatta caaaattgta gccagctata aaaagcgcga taaaataaag aaaaggctcg
      421 ctaaaataac cgtcattgcc gcccgtttta aacttaaccc gacaaatttg taggaatttg
      481 aaaacgacca aaaattatcg aaacgataaa actccggtat ttattggcta aaaagtaaat
      541 tttaactttt ggcatcgcgg ttgcaataga gaaaaaagga tttattgatt gctcaacttc
      601 cgaaatgatt ttgagcgaaa gttatcgctt ttcggcaaat cgcaaaatac aacttcttga
      661 aggaggcgat aactttcgct caaaatcgaa ttctcatttc tcaaatcccg cgaatttcat
      721 tttccgaaca aattctttaa aatttacctg atggacgaaa cgcttcattt tcaggtcgat
      781 aaaagctcgc acaagctgcg gctcgacgaa tttttattcg acaaaatcag aggcgtcagc
      841 aaaatgtacc tgcgcgattt gataaaaacc gaaaaatgtc tggtcaacgg cacggtcgaa
      901 aatcgcggtt ataaattgcg gacaaacgat tttgtcgaaa tcgagatgga ccggcgcgtc
      961 gagcgcgcaa tgaagcccga accgctgccg ctggaaataa ttttcgaaga cgcggaaata
     1021 atcgtcgtca ataaagcggc gggaatgctg atgcacccga cgtttgcgca aaaaaccgga
     1081 acgcttttaa atgcgctggc tttttatctg aatttttccg cgcacagctg cgaagactca
     1141 gagaaaaaca caaaggacaa aggacaagga aaaggggacg aatcaaaatt cattcgtccg
     1201 ggcttgattc atcgcctcga ccgccagact tccggcttgc tggtcgtcgc caaaacgccg
     1261 cgcgcgcacc gcgttttatc tgaccatttc cggcgaaagt tagtcgaaaa aagatatttc
     1321 gcgctggtcg aaggcgtcgt cggggaagat tcgggaacaa tcctcgcgcc gatcggacgc
     1381 attgaggaaa tcaggttgtg gaacgtcaag gaagacggta aacacgccga aacccgcttc
     1441 cgggtcaaag aaagattttc cgattccact cttctcgaac tcgaacccgt caccggcaga
     1501 acaaatcaac tgcggattca ctgcgctcac atcgggcacc cgattatcgg cgacgaatgg
     1561 cacagcaggc gcgaatttcc gcgtctgtgc ctgcacgctt ataaactggc gttctggcat
     1621 ccggacggga atagatggat ggaatttgag cccgcgcgtg aaaattattt tgcctgacga
     1681 cgaaaccggc gcgcggctgt tttaactgcc aaacaaattc aaatatttta tccgctcgcc
     1741 gataaaccag ttcaaatttt cgctcggcgc ggtcttgttc gattcataga caaaaataaa
     1801 atttccgccg ccgtcgatgt tcaccgtcgg taattgatca aaatacgaca gcatttgcgg
     1861 gtgaaatgtc tgccgcacag ccctctcgtc tccgcc
//
LOCUS       DASWNE010000792         1524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_165867, whole genome shotgun sequence.
ACCESSION   DASWNE010000792 DASWNE010000000
VERSION     DASWNE010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165867"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1471)
                     /locus_tag="VGB68_15395"
     CDS             complement(<1..1471)
                     /locus_tag="VGB68_15395"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0050677.1"
                     /translation="MSYCHECGSEVAKDDVYCPYCGIALQNDAGVSTTTVATPDSALP
                     FEATQLDVPLESPIQPVQQSEEVVIKDISLPAMPKDFDSGRHSLDTPLKTDTSENIAF
                     QTDEAIGLESNLSNAESAAPQTGEAPVFAPLAQPEIYLPAAENEAAGKIDQPNFESVP
                     PMASENAADQPGIGETGGEESAPPNVENQTPEPDFDSTIAYTPADIAQQEAVASKLAE
                     NQAAAPQKETAPAESENEIKPPATPLAEVEEEDLPATLISEQLPPFEQKAPDAARYAQ
                     QNQPLAEKSDVQISEPDMPISEPVEKASEVSASDAEKSGDVQAARSNTSPNVNSIDTG
                     GKKAKLKTLSEGTLLNGRYEVVRKIGGGGMGAVYLASDKNLGGVLRAVKEMVQSYIEE
                     TQQEKAINDFKRESLLLTSLEHSSIPTIYDYFYDEKEGRFYLVMKYISGGDLASRLRS
                     SPEGRIDEQSVTEWAIQVADVLDYLHNRQPPIVYRDLKPS"
BASE COUNT          281 a          409 c          430 g          404 t
ORIGIN      
        1 tcgacggctt taaatcgcgg taaacaatcg gcggctggcg gttgtgcagg taatccagca
       61 cgtcggcgac ctgaatcgcc cattcggtga cgctctgctc gtcaatcctg ccttccggcg
      121 acgagcgcag gcgcgacgcc aaatcgccgc ccgaaatata tttcatcacc agatagaatc
      181 tgccttcttt ttcgtcgtaa aaataatcgt aaatcgtcgg aatcgaagaa tgttcgagcg
      241 aagtcagaag cagggactcg cgcttgaaat cgttaatcgc tttttcctgc tgcgtttcct
      301 cgatgtagga ctgcaccatt tccttgacgg cgcgcagaac gccgcccagg tttttgtcgc
      361 tggcgagata aaccgcgccc attccgccgc cgccgatttt gcggacgact tcgtaacgcc
      421 cgtttaagag cgtgccttcg gagagtgttt tcagcttggc tttcttgccg ccggtatcaa
      481 tcgaattgac gttcgggctg gtgttggagc gcgcagcttg tacgtcgccc gatttttccg
      541 cgtcagaagc ggaaacttcg gacgcttttt cgaccggctc ggaaatcggc atatcaggct
      601 cggaaatctg aacatcgctt ttttccgcta acggctgatt ttgctgagcg tatcgcgccg
      661 catccggcgc tttctgctca aacggcggaa gctgctcgga aatcagcgtc gccggaaggt
      721 cttcttcttc gacttcggcg agcggcgtcg cgggcggctt tatttcgttt tcagattccg
      781 ccggcgcggt ttctttttgc ggcgcggcgg cttgattttc cgccagtttc gaggcaaccg
      841 cttcctgctg cgcaatatcg gcgggcgtgt aggcaatcgt cgaatcgaaa tccggttcag
      901 gcgtttgatt ttcgacgttc ggcggcgcgg attcttcgcc gccggtttcg ccgatgcccg
      961 gctggtctgc ggcgttttcg ctcgccattg gcggaacgga ttcaaaattc ggctggtcga
     1021 tttttccggc ggcttcgttt tccgctgccg gcagataaat ttcgggctgc gctaaaggcg
     1081 caaaaacggg tgcttctccg gtttgcggcg cggcgctctc ggcgtttgaa agatttgatt
     1141 ccagcccgat ggcttcgtcg gtctgaaaag cgatattttc ggacgtgtcg gttttcagag
     1201 gcgtgtcgag cgaatgcctg ccgctgtcaa aatccttcgg catcgcgggc agcgaaatat
     1261 cttttatgac gacctcttcg ctttgctgca ccggctggat aggactttcc aaaggcacgt
     1321 ctaattgcgt cgcttcaaac ggcagagcgg agtccggcgt ggctacggtc gttgtcgaga
     1381 ctcccgcgtc gttttgcaga gcgattccgc aataagggca gtaaacatca tctttggcga
     1441 cttccgagcc gcattcgtga caataggaca ttattttttg ttagctagtt caaatttatt
     1501 ggtttatgcg aaaacgtaaa aatg
//
LOCUS       DASWNE010000793         1702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_378070, whole genome shotgun sequence.
ACCESSION   DASWNE010000793 DASWNE010000000
VERSION     DASWNE010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378070"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1527)
                     /locus_tag="VGB68_15400"
     CDS             complement(<1..1527)
                     /locus_tag="VGB68_15400"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEY0050678.1"
                     /translation="MNDTTLTTSLPTINVSGILENEPPEVKSAKALAMRYRLPYIDLL
                     PPDKKSPIDYEALANIPVEMMLRNHFVPLKKEGRNLHVAMADPTNLEQLDELENALNV
                     RIVPYVSTLGAIEVILKKGDATQRVLQEAASTFKISLVKETDQGEEVLDLDRLASDSD
                     MSPIIKLVDTIIYNAMESRASDIHIETRERDVQVKFRIDGALYAKVDPIDIAYHQTLI
                     SRIKVMSELDIAERRIPQDGRFRVRYKGRTVDFRVSIMPTVFGEDAVIRILDKEQISE
                     EFKNLSLEVVGFNEDDLKHFRVYIKEPYGMVLVTGPTGSGKTTTLYGALNEIRNDEDK
                     IITIEDPVEYQLHGIVQIPVNEKKGLTFARGLRSILRHDPDKIMVGEIRDEETAQIAI
                     QSALTGHLVFTTVHANNVIDVIGRFINMGVEPYNFVSALNCVLAQRLVRKLCATCKRA
                     YEPSEDELRDSGLPPAAVVDKHIFMSVGCDACNHTGYRGRTAIHELLDMSDTIREMIV
                     E"
BASE COUNT          380 a          448 c          442 g          432 t
ORIGIN      
        1 ctcgacgatc atttcgcgaa tcgtatcgga catatccaaa agctcgtgaa tcgccgttct
       61 gccgcgatag cccgtatgat tgcacgcgtc gcagccgacg ctcataaaaa tgtgtttatc
      121 gacgaccgcg gcgggcggca agccggaatc gcgcagctcg tcctcgctcg gctcataagc
      181 gcgcttgcag gtcgcgcaga gctttctgac cagacgctgc gccagaacgc agttgagcgc
      241 ggaaacgaag ttgtacggct cgacgcccat attgataaat cgcccgataa cgtcgatgac
      301 gttgttggcg tggacggtcg taaaaacgag gtgtccggtc agcgccgact gaatcgcaat
      361 ctgcgcggtt tcctcgtcgc ggatttcgcc gaccatgatt ttgtccgggt cgtgacgcag
      421 gatggagcga agcccgcgag caaacgtcaa accttttttc tcgttgaccg gaatctgcac
      481 gatgccgtga agctggtatt cgaccgggtc ttcgatggtg atgattttgt cttcgtcgtt
      541 gcggatttcg tttaaagcgc cgtaaagcgt cgtcgtttta cccgaaccgg tcggacccgt
      601 caccagaacc atcccgtaag gctctttgat gtaaacgcgg aaatgtttta aatcgtcctc
      661 gttaaagccg acgacttcga ggctcaaatt tttaaattct tcggaaatct gctctttgtc
      721 gagaatacgt ataacggcgt cttcgccgaa aacggtcggc ataatcgaga cgcggaaatc
      781 gaccgtgcga cctttgtatc gaacgcgaaa cctgccgtcc tgcggaatgc ggcgctcggc
      841 gatgtccagc tcggacatca ccttgatgcg cgaaatcaaa gtctggtgat aggcgatatc
      901 aatcgggtcg acctttgcgt acagcgcgcc gtcgatgcga aatttgacct gcacgtcgcg
      961 ctctctggtt tcgatgtgaa tatcggaagc gcgcgattcc atcgcgttgt agataatcgt
     1021 gtcgaccaat ttgataatcg gcgacatatc cgaatccgac gccaggcggt cgaggtcgag
     1081 aacttcttcg ccctggtcgg tttccttgac gagcgagatt ttaaaggtcg aggcggcttc
     1141 ctgcaagact ctctgcgtcg cgtcgccttt tttcaaaatg acctcgattg cgccgagcgt
     1201 cgaaacatac ggcacgatgc ggacgttcag agcgttttcc agctcgtcga gctgctccag
     1261 attcgtcggg tcagccatcg ccacgtgcag atttctgcct tcttttttga gcgggacgaa
     1321 atgattgcgc agcatcatct cgaccggaat attcgccagc gcctcgtaat caatcggcga
     1381 ttttttgtcc ggcggcagca ggtcgatata cggcaggcga tatcgcatcg ccaaagcctt
     1441 cgccgatttc acctcgggcg gctcgttttc cagaatcccc gaaacgttaa tcgtcgggag
     1501 cgaggtcgtc aatgttgtat cgttcataaa tttagcgatc agctatcagc catcagcgtt
     1561 cagcttttgg atgaatgaag ctaacatttt tttaatttcc agtaacaaat tttccataac
     1621 cttataactt tctaaattta ataacttaag gtcattagct aaaagtaagt gatattctaa
     1681 ttcacttgcc gagccgaatg ct
//
LOCUS       DASWNE010000794         3326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_26984, whole genome shotgun sequence.
ACCESSION   DASWNE010000794 DASWNE010000000
VERSION     DASWNE010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26984"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VGB68_15405"
     CDS             <1..521
                     /locus_tag="VGB68_15405"
                     /inference="COORDINATES: protein motif:HMM:NF042732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6683 family protein"
                     /protein_id="HEY0050679.1"
                     /translation="VKQSNASAQQKADAVKIIEQMVIIYADTATKDGFPANDLAYAFQ
                     YYVVQNYHAYHNVFENPVVIPYTNTIDVSKTPNYISSFGEKALFNQFKAVLTQNPSVT
                     KLSDLQKQQYTELLAVITNINFTAYSEGLKRKDRKIIEQARAAAKQNLERLFGVSADK
                     IVIGDAGLSFKK"
     gene            548..1288
                     /locus_tag="VGB68_15410"
     CDS             548..1288
                     /locus_tag="VGB68_15410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050680.1"
                     /translation="MAFGISFVDIELLIRLSRNQMAESEPVLISGEAAISPEAIISVL
                     SETSFSSDSFSANNSGTSLVAALTENIPPVSLAADSGAEKSTESCDEENYKAAAIVFN
                     LTSRIAASSIKQSTTGEMPAANNFSAARALRQNQLDAEMIFRRLLEKFRTSVIYDYEA
                     GDAVRLEEDAARKHKPFESFSRRFADSNENRAETNRKKAIRDKENLKSEALRKTGDDF
                     ENQSSDERIRESKKRELLKKIREANKFL"
     gene            1505..>3326
                     /locus_tag="VGB68_15415"
     CDS             1505..>3326
                     /locus_tag="VGB68_15415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0050681.1"
                     /translation="MSFNESESHIENRFRAILEQSPFSIQIMSPDGYTIQVNKAWEQL
                     WGVTLEQIKGYNILDDEQLIKKGIMPYIRRGFDGEATEIPPILYDPEESIPEMSRHAE
                     PKRWTKAVIYPIKDSGGKVVEVVLMHEDITAQMLAEEKLKMSEERYRMLFETTLDCVM
                     VVDEQGKYIDVNESLCRKLKASREQLIGSHFSEYMVPERLGEARKAFVALRDTGVYEG
                     EFPMRAADGSIIEFEWISRANFMPGLHVCFARDVTESKLAEEALRESETRYRSLLENA
                     NDIIYSHDLKGNYLSINRAGEIITGYTREEVLGGLNIAQLIAPEHLQLAKEMTARKLR
                     DPQPTIYEIDIINKEGRRLTLEVSTRVSYNNDVPVAVEGVARDVTERKRVEKEKAQLA
                     EQIESQRKHLQAMVSSVPGVVWEAWGEPDENNQRIDFVSEYVEKMLGYSVEEWLSTPN
                     FWLSIVHPEDREIAASNAVETFKSGEPGINEFRWIAKNGRAVWVESQSVAIFDERGIP
                     IGMRGVTMDISERKQKQAAEKFLAEASTTLASSLEYETTLCTVAQLAVPHFADWCSVD
                     IANEEGTLSRLAVAHIDPEKVSWAQEIAKRYPPDRTAPQGI"
BASE COUNT          911 a          844 c          884 g          687 t
ORIGIN      
        1 tggtcaaaca atcgaacgcg tcggcgcagc aaaaagccga cgcggtgaaa attatcgagc
       61 agatggtgat tatctacgcc gacacggcga cgaaagacgg ctttcccgcc aacgatttgg
      121 cgtacgcttt tcaatattac gtcgtgcaga attatcacgc ttatcacaac gtttttgaaa
      181 atccggtcgt cattccctac acgaacacga ttgatgtcag caaaacgccg aattacatca
      241 gcagcttcgg cgaaaaagcc ctgttcaatc aattcaaggc ggtgctcacg caaaacccgt
      301 cggtgacgaa actgagcgat ttgcagaaac agcaatacac ggagctgctc gccgtcatca
      361 cgaacataaa tttcacagcc tatagcgaag gcttgaaaag aaaagaccgg aaaatcatcg
      421 agcaggcgcg ggcggcggcg aaacaaaatc tcgaacgctt gttcggcgtt tcagccgaca
      481 aaatcgttat cggcgatgcg ggcttaagct ttaaaaaata gaggttgata aaaaatggag
      541 gttgaaaatg gctttcggga tttcattcgt ggacatagag ttattgattc gcctttcgcg
      601 aaatcaaatg gcggaaagcg agccggtttt gatttccggg gaagccgcga tatcgcccga
      661 agcgataatt tccgttttat ccgaaacgtc ttttagttcc gattcctttt ccgcaaacaa
      721 ttccgggacg agccttgtcg cagcgcttac ggaaaatatt ccgcccgtat ctttagcggc
      781 ggattccggc gcggagaaat cgacggaaag ttgcgacgaa gaaaactaca aagctgccgc
      841 cattgttttt aatttaactt cgcggattgc tgcatcgagc ataaaacaat caacgaccgg
      901 cgagatgccg gcggcgaata atttttccgc cgcgcgagct ttaaggcaaa atcaattgga
      961 cgccgagatg atatttcgcc gtctgctgga aaaattcaga acgtcggtca tttacgatta
     1021 tgaagcaggc gacgccgttc ggcttgaaga agacgcggcg agaaaacaca aaccgttcga
     1081 gagtttcagc cgccgctttg ccgattcaaa cgaaaaccgc gccgaaacga acagaaaaaa
     1141 agcgattcgg gacaaagaaa atcttaaatc ggaagcttta agaaaaaccg gcgacgattt
     1201 tgaaaatcaa tcgagcgacg agcgaatccg cgaaagcaaa aaacgagagc tgttgaaaaa
     1261 aattcgtgag gcgaataaat ttctctgaga tttaccgttt cgaaatttat atcgggttca
     1321 ggcgattcgg cgcgaatcgc cttttttatt ttctgctgta accgcggcaa agaaacgcag
     1381 cggcacggcg ctgaatttct ttgccgcggt tcgatttgaa gcgggcgcaa cacgtcgttt
     1441 attgccttaa tttccctgtt tagcctatga taagaggtgt tcatttttat aagtcgtgaa
     1501 tcccatgtcc tttaacgaat cagaatccca tatcgaaaac cgctttcgcg ccattcttga
     1561 acaatctccg ttcagcatcc agataatgtc gcccgacggt tacaccattc aggtcaataa
     1621 agcttgggaa caattgtggg gcgtgacgct cgaacagatt aaaggctaca acatactcga
     1681 cgacgagcag cttatcaaaa aaggaataat gccttatata cggcgcggat ttgacggcga
     1741 agccacggaa atcccgccga ttctctacga cccggaagag tcgattcccg agatgagccg
     1801 ccacgccgag ccgaagcgct ggacgaaagc cgttatctac ccgattaagg attccggcgg
     1861 aaaggtggtc gaagtcgtgc tgatgcacga ggacattacc gcgcagatgc tcgccgaaga
     1921 gaaattgaaa atgagcgagg agcgctaccg gatgcttttc gagaccactc tggactgcgt
     1981 tatggtggtc gacgagcagg gcaaatatat cgacgtcaac gaaagcctgt gccgcaaact
     2041 gaaagcctcg cgcgagcagt taatcggctc tcatttcagc gaatatatgg ttccggaaag
     2101 gctcggagaa gcgcggaagg cgtttgtcgc tttgcgcgac acgggcgttt acgaaggcga
     2161 attcccgatg cgcgccgccg acggcagcat tatcgaattc gaatggattt cgcgcgccaa
     2221 ttttatgccc ggactgcacg tctgtttcgc gcgcgacgtg actgaaagca agctcgccga
     2281 agaagcgctg cgcgaaagcg aaacgcgtta ccggagcctt ctggaaaacg ccaacgacat
     2341 tatttattca cacgacctga aaggcaatta tctttccatc aaccgcgcgg gcgagataat
     2401 aaccggctac acgcgcgaag aagtgctcgg cggcttgaat atcgcgcagc tcatcgcgcc
     2461 cgaacatctg caactggcga aagagatgac cgcgcgcaaa ttgcgagacc cgcagccgac
     2521 catctacgag attgacatta tcaataaaga aggtcgccgc ctgacgctcg aagtcagcac
     2581 gcgggtttca tataacaacg atgttccggt cgccgtcgaa ggcgtcgcgc gcgacgtcac
     2641 cgagcgcaag cgcgtcgaaa aggaaaaagc gcagcttgcc gagcagatcg aatctcagcg
     2701 aaagcacctg caggcgatgg tttcgagcgt tcccggcgtc gtctgggaag cgtggggcga
     2761 gccggacgaa aataatcagc gcatcgattt cgtcagcgaa tacgtggaaa aaatgctcgg
     2821 ctacagcgtc gaggaatggc tctcgacgcc gaatttctgg ctctcaatcg tgcatcccga
     2881 agacagagaa atcgccgcga gcaatgccgt cgagactttt aaaagcggcg agccgggcat
     2941 caacgagttt cgctggattg ccaaaaacgg gcgcgcggtc tgggtcgaat cgcaatccgt
     3001 cgctattttt gacgagcgcg gaatcccaat cggaatgcgc ggcgtaacga tggatatcag
     3061 cgagcgcaaa cagaagcaag ccgccgaaaa atttctcgcc gaagccagca cgacgctcgc
     3121 ttcatcgctc gaatacgaaa cgacgctctg cacggtggcg cagcttgccg tgccgcattt
     3181 cgccgattgg tgcagcgtcg atattgcgaa tgaagaggga acgctgagcc gtctggcggt
     3241 cgctcacatc gacccggaaa aagtttcgtg ggcacaggaa atcgctaagc gttatccgcc
     3301 tgaccgaacc gcgccgcaag gcatct
//
LOCUS       DASWNE010000795         1756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_274850, whole genome shotgun sequence.
ACCESSION   DASWNE010000795 DASWNE010000000
VERSION     DASWNE010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274850"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1405
                     /gene="recG"
                     /locus_tag="VGB68_15420"
     CDS             <1..1405
                     /gene="recG"
                     /locus_tag="VGB68_15420"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008165.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEY0050682.1"
                     /translation="EFFWVSFALQLKRGERAKDPKGAIIELNETIRERLRAVLPFTLT
                     GAQERVVERIFADMISDSPMNRLVQGDVGSGKTIVALLAMLAAMENGYQTALMAPTEI
                     LAEQHARNFKRLLAATPFTTELLIGSLRAAEKRKVQQMIAEGAVDAVIGTHAVIQDAV
                     AFQKLGLAVIDEQHRFGVLQRAELRARGYSPDILVMTATPIPRSLAMTVYGDLDVSVI
                     DELPPGRTPIKTVVVGEDKRDGVYKGIVREIELGRQVYIVYPLVEESEKMDLKAATAM
                     FEELRDKVFPNYKIGLLHGKMKAGEKEAVMQSFTEGDIQILVSTTVIEVGVDVPNASL
                     MIIEHAERFGLSQLHQLRGRVGRGAEQSYCVLLTNDKKTAVAKERLGIMEETTDGFRI
                     AEKDLELRGQGEILGTRQSGVQTFRIGNIIRDLEILETARKEAEFYLTTKRFTQETSK
                     LIELARSDARFKLAGIG"
BASE COUNT          439 a          415 c          500 g          402 t
ORIGIN      
        1 agaatttttc tgggtctcgt ttgccctgca attaaaacgc ggcgagcgcg ccaaagaccc
       61 gaaaggcgcg attatcgagc tgaacgaaac gattcgggaa aggctcagag ccgttttgcc
      121 gttcacctta acgggcgcgc aggagagagt cgtcgaaaga attttcgccg atatgatttc
      181 cgattcgccg atgaatcgtc tggtgcaggg cgatgtcggc agcggaaaaa cgatcgtcgc
      241 gctgctggcg atgctggcgg cgatggaaaa cggttatcaa accgccctga tggctccgac
      301 cgaaatcctt gccgaacagc acgcgcgaaa cttcaaacgg cttttagccg ccacgccttt
      361 tacgaccgag cttttaatcg gcagcctgcg cgcggcggaa aaacgcaaag tccagcagat
      421 gattgccgaa ggcgcggtcg acgcggtcat cggcacgcac gccgttattc aggacgcggt
      481 cgcgtttcag aaattaggtt tagccgtgat tgacgagcag catcgtttcg gcgttctcca
      541 gcgcgccgag ttgcgcgcgc gcggctacag cccggatatt ctggtgatga cggcgacgcc
      601 gattccgcgc agcctggcga tgacggttta cggcgatttg gacgtttcgg tgattgacga
      661 gctgccgccc ggtcggacgc cgataaaaac ggtcgtcgtc ggcgaggaca agcgcgacgg
      721 cgtctacaag ggaatcgttc gcgaaatcga gctcgggcgg caggtttaca tcgtttaccc
      781 gctggtcgaa gaatcggaaa agatggattt aaaagcggcg acggcgatgt ttgaagaact
      841 gcgcgacaag gtttttccaa actacaaaat cggtctgctg cacggcaaaa tgaaagcggg
      901 cgaaaaggaa gccgtgatgc agagctttac cgagggcgac atacagattc tcgtttcgac
      961 gaccgtcatc gaagtcggcg tcgatgttcc gaacgccagc ttgatgatta tcgagcacgc
     1021 cgagcgtttc ggtttgtcgc agctacacca gctgcgcggg cgcgtcgggc gcggcgcgga
     1081 gcaaagctac tgcgtgcttt tgacaaacga caaaaaaacg gcggtcgcca aggagcggct
     1141 gggaattatg gaagaaacga cggacggttt tcgcatcgcc gaaaaggatt tggagctgcg
     1201 cggtcagggc gagattctag gaacccggca atcgggcgtg cagacgtttc gcatcggcaa
     1261 tatcatccgc gatttggaga ttctggagac ggcgcgcaag gaagccgaat tttatctgac
     1321 gaccaagcgc tttacgcagg aaacttcaaa attaatcgag cttgccaggt cggacgcgcg
     1381 ctttaagctg gcgggaatcg ggtaataaaa taaacgcgga atgatgaaag agaaacgccg
     1441 aataaagaat ttgccgactg tcgtcccgcg ttcgcgcgtt tcgttttatc cgggtttaac
     1501 tacctaaatg ggtggtttca ttttcctttc attgtgttac ttatatcttg tcaggaatat
     1561 ttcggaatcg ggaattttta aaaaaaagta atttttcgta aagagtgatg aaaagcattg
     1621 aattttttcg cctaaatcgt ttatattcgt ctgtaacagt ttctctaaat catttataaa
     1681 ctaagagaat cttaattgat tgcaaaatgc tcgactgaat tttctcaaaa attcccaatt
     1741 ttagttcgga caattt
//
LOCUS       DASWNE010000796         1664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_27093, whole genome shotgun sequence.
ACCESSION   DASWNE010000796 DASWNE010000000
VERSION     DASWNE010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27093"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="VGB68_15425"
     CDS             <1..786
                     /locus_tag="VGB68_15425"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HEY0050683.1"
                     /translation="RYMRNGRDLGQWVHIDVLFQAYFQAFLVISGLGVPADDGNPYKN
                     SATQIGFGTFGGPHIATLLCEVSTRALKAVWNQKWFVHRRLRPEVFAERVDRRAYHGA
                     NYPVHQEILNSVTTATRLGGYLPAGNAFLPQAFPEGSPTHPAYGAGHATVAGACVTIL
                     KAWFNENTVIQNPVVPDSTGTSLVSYSGSENLTVGGELNKMAANVALGRNTAGVHWRS
                     DGTESMLLGEALAISILRDQKTGYNEQFNGFSLTKFDGTTITV"
     gene            864..>1664
                     /locus_tag="VGB68_15430"
     CDS             864..>1664
                     /locus_tag="VGB68_15430"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050684.1"
                     /translation="MPKRTDIHKILIIGSGPIVIGQAAEFDYSGTQACGALKQEGFEV
                     VLVNSNPATIMTDPEIADKTYVEPLTIETVSAIIEKERPDALLPTVGGQTGLNLSVAL
                     YESGILDKFGVKLIGANVASIKVGEDRELFKKAMDEIGIPSPKGGFAHTWEEAQKIVE
                     ATGYPAIVRPSFTLGGTGGGTAFNPEEFEEIAKGGLAASPTSQILVEESILGWKEYEL
                     EVMRDLNDNVVIICSIENFDPMGVHTGDSITVAPAQTLTDVEYQRLRDM"
BASE COUNT          402 a          445 c          457 g          360 t
ORIGIN      
        1 cgctatatgc gcaacgggcg cgacctcggg caatgggttc acatcgacgt tttgttccag
       61 gcgtattttc aggcgtttct ggttatttcg ggcttgggcg ttccggcaga cgacggaaat
      121 ccgtataaaa attcggcgac gcaaatcggt ttcggcacgt ttggcggacc gcacatcgcc
      181 acgcttttat gcgaagtctc gacgcgcgcc ctgaaagcgg tctggaatca aaaatggttt
      241 gtccatcgcc gtctgcgacc ggaagttttc gccgaaagag ttgaccgcag agcttatcac
      301 ggggcgaatt acccggttca tcaggaaatt ttaaattccg tcacgaccgc cacgcgcctc
      361 ggcggatatt tgcccgccgg aaacgctttt ctgccgcagg cttttcccga aggctcgccg
      421 acgcatccgg cttacggcgc gggacacgcg acggttgccg gcgcctgcgt aactattctg
      481 aaagcgtggt ttaacgaaaa caccgtcatt caaaacccgg tcgtcccgga ctcgaccgga
      541 acgtctctgg tttcctacag cggctcggaa aatttaaccg tcggcggcga attaaataaa
      601 atggctgcca acgtcgcgct cggacgcaac acggcgggcg ttcactggcg ctcggacggc
      661 acggaatcga tgctgctcgg cgaagcgctc gccatcagca tcctgcgcga ccagaaaacc
      721 ggctacaatg aacaattcaa cggttttagc ctgaccaaat tcgacggcac gacgattacg
      781 gtttagcgaa aagctttgaa tcgataaaaa gaaagacgcg gttatattga ccgcgttttt
      841 ttctgtataa tcgaaaattt aatatgccga aacgcacaga cattcataaa atcctaatca
      901 tcggttcggg tcctatcgtc atcggtcagg cggcggagtt cgattattcg ggaacgcagg
      961 cttgcggcgc tctgaaacag gaaggctttg aagtcgtact ggtcaattcc aatccggcga
     1021 cgattatgac cgacccggaa attgccgata aaacttacgt cgagccttta acaatcgaga
     1081 ccgtcagcgc gattatcgaa aaagaacgcc ccgacgcgct tttgccgacg gtcggcggtc
     1141 agaccggttt gaatttatcg gtcgctttat acgaatcggg aattctggac aaattcggcg
     1201 tcaagcttat cggcgcaaac gtcgcgtcaa tcaaagtcgg cgaagaccgc gagcttttca
     1261 aaaaagcgat ggacgaaatc ggtattccca gcccgaaagg cggcttcgcg cacacctggg
     1321 aagaagcgca gaaaatcgtc gaggcgacgg gttatccggc gattgtccga ccgagtttta
     1381 cattgggcgg cacgggcggc ggcacggctt tcaatcccga agaattcgag gaaatcgcca
     1441 aaggcggttt agccgcttcg ccgacctcgc agattctggt cgaagaatcc attttaggct
     1501 ggaaggaata cgagctggaa gtgatgcgcg atttgaacga taacgtcgtc attatctgtt
     1561 caatcgaaaa cttcgacccg atgggcgtgc acaccggcga ttcgataacg gtcgcgcccg
     1621 cgcagacctt gaccgatgtc gaataccagc gcctgcgcga tatg
//
LOCUS       DASWNE010000797         7369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_592214, whole genome shotgun sequence.
ACCESSION   DASWNE010000797 DASWNE010000000
VERSION     DASWNE010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7369
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592214"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VGB68_15435"
     CDS             complement(<1..382)
                     /locus_tag="VGB68_15435"
                     /inference="COORDINATES: protein motif:HMM:NF026035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050685.1"
                     /translation="MKKNFRAACLTALLSALFFQLFSQNLPAQRKIVPPQKSVEVFSV
                     SGVDIPEKLSPEAERRLETFRLVWQTVKDNYFDQSFGGLNWDKIKTEFEMRSLGSRTD
                     AELHLILQEMINRLNKSHFVIIPPE"
     gene            complement(622..1629)
                     /gene="gap"
                     /locus_tag="VGB68_15440"
     CDS             complement(622..1629)
                     /gene="gap"
                     /locus_tag="VGB68_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEY0050686.1"
                     /translation="MGIKVGINGFGRIGRNVLRTCIGDADVDFVAVNDLTDTRTLAHL
                     LKYDSIMGNLSNDITHTDDTIKVDADEFRVFSEKDPSLIDWESVGAEIVIESTGRFTK
                     AEDAAKHLRGSVKKVIISAPAKGEDVTIVLGVNEDMYDPENHNIISNASCTTNCLAPV
                     AKVIHEKFGIRNALMNTIHSYTNDQQLLDLPHKDLRRARAAALSMIPTSTGAAKAVAL
                     VIPELKGKFDGISVRVPTPNVSLVDVVMNVEKSTSTEEVNQVLKDAANGELKGILAFS
                     EEPLVSIDFRGNPNSSIVDAENTKVIDGTAIKILSWYDNEWGYSCRVRDLIKFIAAKG
                     L"
     gene            1941..3752
                     /locus_tag="VGB68_15445"
     CDS             1941..3752
                     /locus_tag="VGB68_15445"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY0050687.1"
                     /translation="MKLFYRTAFFALALSLSTGLCDLRSVQAQTPATVPQIAKNSIEP
                     SVEKSSEKPEEKIEKPVESNNVNILHRVGVQTSATLPLTLNEAIRRALENNNAIEVAR
                     DDVRFQETQLRSQLGIYDPVFTVSPTLTRNSTTGSRATNDFRLNSDVSGLVRRGGGNY
                     RVFFDNVRTENSFSQQQLSSGNTATGGALYSSGIGVGYTQPLLRDFRIDNTRRQIKIQ
                     RRFLQQTDADFRRQTIDVIAQVQRGYWDLVFALRDQQNRVANLNLARENLRQVEAKIE
                     AGSSAPLERAEVATELANREGEVLQSTQQVTVAENNLKTLLLRDIAAPEWNSSLVPTD
                     RPVYSDETIALDDAIKDAVENRPELQRLKLAREINSINIDYFKNQTKPQLDLNTTFSL
                     NGFSQGIQNNSEFTVPQFTGNDEILRLRLNTLLPPNMQIDNPLIVVPASPGFLVGGFN
                     RSFLNLFRSDAPNYTVGVTISFPLRNRTARANLAGARVQEEQLSAQTRAQEQTIIADV
                     RNAVQAVETARQRVLVARRARANAEIQLEGERKKYEVGFSSTFLLFQRENALANARNA
                     EIRAETDFNKSLADLQRATSTTFRANNIEVESPVNDK"
     gene            3956..4744
                     /locus_tag="VGB68_15450"
     CDS             3956..4744
                     /locus_tag="VGB68_15450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0050688.1"
                     /translation="MKISVVLIAFNEEKNIRRAIESADWADEILVVDSESVDRTREIA
                     ESLGAKVVVRKWLGFAAQKQFAVDCAANEWIFSLDADEKISAELTEEIRCLKNSSPAA
                     DGYKIPRLSFYMNRPIRRGGWYPDWQLRFFDRRKGNWKDVLIHESVEMNAGARIEKLK
                     GDILHYSVENAAHHHRMIGERYAPLAARRMFEDGRRTSRSRIFFAGLLAFVQTYFLKL
                     GFLDGFAGFCIARFAAHHAFLKHLLLWELQTEGEKPETETGKTN"
     gene            4849..5484
                     /locus_tag="VGB68_15455"
     CDS             4849..5484
                     /locus_tag="VGB68_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050689.1"
                     /translation="MQKIFFSISAVIIFSAFAFGQQQGGKGIDSQTRQINQDNNKATS
                     RPNDAGGRSWDWGKEKTKVRERLANPYRMAARRDVLVENILNLLKERKIIVDDASSRV
                     SDGFIVTQPFLFAKGSVTSENELNRYAVLPAFSNVWTRGRYTLTIEIQSIDGIQNNVA
                     VTAKVEGRTANGIGSEWTTLQSSGAAEDEFLSRLVENVTGKSLETEPVNQQ"
     gene            5494..6024
                     /locus_tag="VGB68_15460"
     CDS             5494..6024
                     /locus_tag="VGB68_15460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050690.1"
                     /translation="MATRFSQLIFLFFVMGFAALGVNAQLPDASSRDPRTGRPEELPK
                     ALKENIQKGRIEEEKKEYEEMIKRGEEAAKLSEELSKSFEQSQTLSPEDVKKLARLEK
                     LVKKIRDDLGGEDDKDIDASKPSSLMNALKSVQENASSLLSALKKTTRLTISAVAIES
                     SNAVLKLVKFIRFYKN"
     gene            6090..6956
                     /locus_tag="VGB68_15465"
     CDS             6090..6956
                     /locus_tag="VGB68_15465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0050691.1"
                     /translation="MLFTLCISVNAQDDDEVIKVDSSIVLVNATVSDAKGKPAAGLKQ
                     NQFKLFVDGAEQEILSFTTESTPFAAIILLDTSGSMEQRVSLARSAAMQFLDGLRADD
                     LTAIYNFDSKVSLVQDFSNSRDIVPTIFDLKAYGMTVLNDAIYKAAQELQNRPERRRA
                     IIVLSDGADTKSARSADKALKAALSANATIYTVDMSSVEANSRNRMQNQGVLKNFAEK
                     SGGRFIPTPGGIAMREAFKNIVAELGRQYTLSFQPPESKNDGKWHTIELKHSNPELTV
                     RTRKGYNAPKKR"
     gene            complement(7030..7311)
                     /locus_tag="VGB68_15470"
     CDS             complement(7030..7311)
                     /locus_tag="VGB68_15470"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEY0050692.1"
                     /translation="MSVAKNIEITSSSARSFEDAINRGIARASKSLNNIRGAWIKEQK
                     ISIENGQATEYRVMMILTFVLDDDEEAVEEEVKTADTKKAGKAGKGKKK"
BASE COUNT         1870 a         1871 c         1841 g         1787 t
ORIGIN      
        1 cttccggcgg aataatgaca aaatgcgatt tgttcaggcg attaatcatt tcctgcaaaa
       61 tcaggtgcag ctcggcgtcc gtccgcgagc cgagcgagcg catctcgaat tcggttttta
      121 ttttgtccca atttaagccg ccgaagctct ggtcgaaata attgtctttg acggtttgcc
      181 aaacgaggcg aaacgtttcc agacgccttt cggcttcggg cgaaagcttt tcgggaatat
      241 cgactccgga aaccgaaaag acctcgacgg atttttgcgg cggaactatt tttcgctgcg
      301 cgggaagatt ctgcgaaaat aactgaaaaa ataatgccga taaaagagcg gtcaggcaag
      361 ctgcccgaaa attttttttc attggctaat ctttctgaaa tcggattgaa ttttgaaccg
      421 cttgaattta accgcttgaa ggaatgggcg cggacgggtc tcgtcgcgca cagaaagatt
      481 agtttcgttg ggctgttttg tcaataaaat tttgcggaaa attaacaggg atgacaggat
      541 gaacagggat tttaagaaat tgatttatcc ctgctcatcc tgtcatccct gttaattccg
      601 tcctatcggc tgtgctataa attacaagcc tttagccgcg atgaatttta ttaaatcgcg
      661 gacgcggcac gaataacccc attcgttgtc gtaccaggac aaaatcttaa tcgccgtgcc
      721 gtcgatgact ttcgtgtttt cggcatcgac aatcgaagaa ttcgggttgc cgcgaaaatc
      781 aatcgagacg agcggttctt ccgagaaagc taagattcct ttcaattccc cgttcgccgc
      841 gtcttttaaa acctggttga cctcttcggt cgacgtcgat ttttcgacgt tcatcacgac
      901 gtcgactaag gaaacgttcg gcgtcggaac gcggacggaa atgccgtcga attttccttt
      961 cagctccgga atgacgagcg cgacggcttt tgccgcgccg gtcgaggtcg gaatcatcga
     1021 aagcgcggcg gcgcgggcgc ggcgcaaatc cttgtgcggc aaatccagca attgctggtc
     1081 gttcgtgtaa gaatgaatcg tgttcatcaa agcgttacgg atgccgaatt tttcgtgaat
     1141 cactttcgcc acgggcgcga gacagttggt cgtgcaggaa gcgttggaaa taatattgtg
     1201 attttcgggg tcatacatat cttcattgac gccgagcaca atggtcacgt cctcgccttt
     1261 tgccggagcg gaaatgatga cttttttgac cgagccgcgc aggtgtttcg ccgcgtcttc
     1321 ggctttggta aagcgcccgg tcgattcgat gacgatttcc gcgccgaccg attcccaatc
     1381 gatcagcgac gggtcttttt cgctgaaaac gcgaaactcg tccgcgtcga ctttgatagt
     1441 gtcatccgtg tgcgtaatgt cgttcgacaa attgcccatt atcgaatcgt attttagaag
     1501 atgcgccagc gtgcgcgtgt cggttaaatc gttgacggcg acaaaatcaa cgtccgcatc
     1561 gccgatacac gttcgcagaa cattgcgccc gatgcgcccg aaaccgttaa taccaacttt
     1621 aatgcccatt taattaaaaa cctcctgaaa tatttttgtg taataaaaac ttaaagataa
     1681 cttgtttgcc ggacgcgtga caagtgagca aagcagtagg cacgattgcg attttatgcg
     1741 gctcaggcaa aagtgtaaac gggcgcaaag gcgaaagtta acgagcgcgt ctgaagatta
     1801 aacattcgac gcgcccgctg accgccctga tttgcgactg tttttttttg aaactaacgc
     1861 ccggaattta tcgtagtaag gcttgagttg ttaatcgctt tatctactaa aaagttttca
     1921 tatctggagc caattttaag atgaaattat tttatcgcac tgcttttttt gctctcgctc
     1981 tgagtctgtc cacaggtttg tgcgatttac gttcggttca ggcgcagacg cccgcgaccg
     2041 tgccgcagat tgccaaaaac agcatcgagc cgagcgtcga aaaatcttcc gaaaagccgg
     2101 aagaaaaaat cgaaaaaccc gtcgaatcga ataatgtcaa tattttgcat cgggtcggcg
     2161 ttcaaacctc cgccaccttg ccgctcactc tgaatgaagc cattcgccgc gctttggaaa
     2221 acaataacgc gattgaagtg gcgcgcgacg acgtgcgttt tcaggaaacg cagctgcgct
     2281 cgcagctcgg aatttacgac ccggttttca ccgtttcgcc gactctgacg cgcaattcga
     2341 cgaccggctc gcgggcgacc aatgattttc gtctgaattc ggacgtgtcc ggattagtcc
     2401 gtcgcggcgg cggaaattat cgagtctttt tcgacaacgt gcgcacggaa aacagctttt
     2461 cgcagcagca gctgagctcg ggaaacaccg cgacgggcgg cgcgctttat tcgtcgggca
     2521 tcggtgtcgg ctatacgcag ccgcttttgc gcgattttcg gattgacaac acgcgccgcc
     2581 agataaaaat tcagcgtcgt tttctgcagc agaccgacgc cgattttcga cgccagacga
     2641 ttgacgtcat cgcacaggtt cagcgcggct actgggattt ggttttcgcc ctgcgcgacc
     2701 agcaaaatcg cgtggcgaat ctgaatctgg cgcgggaaaa tctgcgacag gtcgaagcca
     2761 aaatcgaagc cggaagctcc gcgccgctcg aacgcgccga agtcgccacc gagcttgcca
     2821 atcgcgaagg cgaagttttg cagtcgacgc agcaggtgac ggtcgccgag aataatctga
     2881 aaacgcttct gctcagagac atcgccgcgc ccgaatggaa cagctcgctg gttccgaccg
     2941 accgaccggt ctacagcgac gagacaatcg cgctggacga cgcgataaag gacgcggttg
     3001 aaaatcgacc cgaactgcaa agattaaaac tggcgcgcga aatcaactcg attaatatcg
     3061 attattttaa aaatcagacc aaaccgcagc ttgatttgaa caccaccttc tctctgaacg
     3121 gattttcgca gggaatccaa aacaactccg agtttaccgt tccgcagttt accggaaacg
     3181 acgagattct gcggctgaga ttgaacacgc tgctgccgcc gaatatgcag attgataatc
     3241 cgctgattgt cgtcccggcg tcgcccgggt ttctggtcgg cggatttaat cgctcgtttc
     3301 tgaatctgtt tcgcagcgac gcgccgaatt acacggtcgg cgtgacgatt tcgtttcctc
     3361 tgcgcaaccg cacggcacgc gccaacctcg ccggggcgcg cgtgcaggaa gaacaactga
     3421 gcgcgcaaac ccgcgcccag gagcaaacga taattgccga cgttcgcaac gcggtgcagg
     3481 cggtcgaaac cgctcgccag agagttctgg tcgcccgccg cgcgcgcgcc aacgccgaaa
     3541 ttcagctcga aggcgagcgc aaaaaatacg aagtcggttt ttcctcgacc tttctgcttt
     3601 tccagcgcga aaacgctctg gcaaacgcgc gcaacgccga gattcgcgcc gagacggatt
     3661 tcaacaaatc gctcgccgat ttgcagcgcg ccacttcgac cacgtttcgc gccaacaata
     3721 tcgaagtcga atcgccggta aatgataaat aagatatcgc ccgcaaaaga cacggaacga
     3781 cggcaaaact tgccgattaa gccttcgtat atttcgcgcg accggtctgg aaatcattta
     3841 taagaccggt taaacattta ttttttaaca gcttaaataa ttttctgaga tttcacttta
     3901 agagtcgctt cgccgactct ttatgttttt cctgaactct gccagaatac caagcgtgaa
     3961 aatttccgtt gttctcatcg ctttcaacga ggaaaaaaat attcgccggg caatcgaatc
     4021 cgccgattgg gcggacgaga ttttggtcgt cgattcggaa agcgtcgacc ggacgcgtga
     4081 gattgccgaa agcctgggcg caaaagtcgt cgtcaggaaa tggctcggat ttgccgcgca
     4141 aaaacaattt gcggtcgact gcgccgccaa cgaatggatt ttcagcctcg acgccgacga
     4201 gaagatttcc gccgaattaa cggaagaaat tcggtgtctg aaaaattctt cgcccgccgc
     4261 cgacggttat aaaatcccgc gcctttcctt ttatatgaat cgaccgattc gccgcggcgg
     4321 ctggtatccc gattggcagc tgcgtttttt cgaccgccgg aaaggcaatt ggaaagacgt
     4381 tttgattcac gaatcggtgg agatgaacgc cggcgcgcga atcgaaaagc taaaaggcga
     4441 cattcttcat tacagcgtcg aaaatgccgc tcatcatcac cggatgatcg gcgagcgtta
     4501 cgcgccgctc gctgctcgcc ggatgtttga agacggcagg cgcacgagcc gttcaaggat
     4561 attttttgcc ggtctgctgg cattcgtcca aacctatttt ctcaagctcg gttttctgga
     4621 cggctttgcc ggattttgca tcgcccgctt tgccgcgcat cacgcttttc tgaaacatct
     4681 gcttctctgg gaattgcaga ccgagggcga aaaacctgag acggaaaccg ggaaaaccaa
     4741 ttgataattt ttactttttc gcttttacaa ctattcaaaa atttctcaca tcaaaccttt
     4801 agttcctgcc gcttttacgc tatcatttga aaaatgcggg ttatttttat gcaaaagatt
     4861 ttcttttcaa tttcggcggt cattattttt tcagccttcg ccttcgggca gcagcaggga
     4921 ggcaaaggca ttgattcgca aacgcggcaa atcaatcagg ataataataa agcgaccagc
     4981 cgaccgaatg acgcgggcgg acggtcgtgg gattggggca aagaaaaaac gaaagtgcgc
     5041 gaaagactcg ccaatcctta tcgaatggcg gcgcggcgcg acgtgctggt cgaaaatatt
     5101 ctcaatcttt taaaggaaag aaaaattatt gttgacgacg cttcgtcgag agtaagcgac
     5161 ggctttatcg tgacgcagcc gtttcttttc gccaaaggtt cggtcaccag cgaaaacgaa
     5221 ttgaaccgct acgccgtttt gccggctttc agcaatgttt ggacgcgcgg tcgttatact
     5281 ttaacgattg agattcaatc tatcgacgga attcaaaaca acgtcgccgt gacggcaaag
     5341 gtcgaaggca ggacggcgaa cggtatcggt tccgaatgga cgacattgca gagcagcggc
     5401 gcggcggaag atgaattttt atccagactg gttgaaaacg tgaccggcaa atcgcttgag
     5461 acggagcccg ttaaccagca gtaaataaag cagatggcta ccagattttc acaattgatt
     5521 ttcctttttt tcgtgatggg ttttgccgct ttgggcgtca atgcccagct tcccgatgct
     5581 tcgtcacgcg acccgcgaac gggcagaccg gaagaactgc cgaaagcgct caaggaaaat
     5641 atccagaaag gtcgtatcga ggaagaaaaa aaagaatacg aagagatgat aaaacgcggc
     5701 gaggaagccg ccaagctcag cgaagaattg agcaaatctt ttgaacaaag ccagacgctt
     5761 tcgccggaag acgtcaaaaa gctcgcgcgg ctggaaaaac tggtcaagaa aatccgcgac
     5821 gatttgggcg gcgaagacga taaggatatc gatgccagca agccttcttc gctgatgaac
     5881 gcgctcaaat cggtgcagga aaacgcttcg agtcttttat ccgctttgaa aaaaaccacg
     5941 cggctgacga tttcggcggt cgccatcgaa agctcaaacg cggttttgaa gctggtcaaa
     6001 tttatccgct tctacaaaaa ctaagtttta aaatgcaaag cgtaaaaagc aaaaggcaaa
     6061 attcaggacg cggtctttta ttttgccttt tgctttttac gctttgcatt tcagtaaacg
     6121 cgcaggacga tgacgaagta atcaaagtag attcgtcgat tgtccttgtc aacgcgacgg
     6181 ttagcgacgc gaaaggcaaa cccgcggcgg gcttaaaaca aaatcaattc aagcttttcg
     6241 tcgacggcgc ggaacaggaa atcctttctt ttactaccga atcgacgcct tttgccgcca
     6301 tcattctgct ggacacttcc ggcagtatgg aacagcgcgt cagtctggcg cgttcagccg
     6361 cgatgcagtt tctcgacggg cttcgcgccg acgatttgac cgccatttac aatttcgatt
     6421 caaaagtctc gctcgtccag gatttttcca acagccgcga catcgtgccg accattttcg
     6481 atttgaaggc ttacgggatg acggttttaa acgacgccat ttataaagcc gcgcaggaac
     6541 tgcaaaaccg tcccgaaaga cgccgcgcca ttatcgttct gtccgacgga gccgatacga
     6601 aaagcgctcg ctcagccgat aaagccctga aagcggcgct ttccgccaac gccacgattt
     6661 acacggtcga tatgtcttcg gtcgaggcga acagcagaaa tagaatgcag aatcagggcg
     6721 ttttaaaaaa tttcgcggag aaaagcggcg gcagatttat cccgacgccg ggcggcatcg
     6781 cgatgcgcga ggcttttaaa aacatcgtcg ccgaactcgg tcggcaatat acattatctt
     6841 ttcagccgcc cgaatcgaaa aacgacggca aatggcacac catcgaactc aaacattcca
     6901 atcccgaatt aaccgtcaga acgcgcaaag gctataacgc gccgaaaaaa agatagctga
     6961 cggaaaacgg gtatcggaaa aaaattttat ctattttccg atacccgttt tccgttttcc
     7021 gtttacgaat tattttttct ttcccttgcc tgctttacca gcctttttcg tatcggcggt
     7081 tttgacttct tcttcgaccg cttcttcgtc gtcgtccaaa acgaaagtca gaatcatcat
     7141 cacgcggtat tccgtcgcct gaccgttctc gatgctgatt ttctgctcct ttatccacgc
     7201 gccgcgaata ttattcaacg atttcgaagc gcgggcgatt ccccgattga tggcgtcttc
     7261 aaagcttctg gcagaagacg acgtaatctc gatgttttta gctacggaca taaaatttct
     7321 ccctgaaaat tagcgatttg ttttgttcct ggatttatca ttatgccta
//
LOCUS       DASWNE010000798        20357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_274906, whole genome shotgun sequence.
ACCESSION   DASWNE010000798 DASWNE010000000
VERSION     DASWNE010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 20357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20357
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274906"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..2717)
                     /locus_tag="VGB68_15475"
     CDS             complement(9..2717)
                     /locus_tag="VGB68_15475"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0050693.1"
                     /translation="MKINATKLLMTLLLGVFSLFSATHAQTTTRISGTVTDQNNAVIV
                     GAQVILRGTISRAVVTNERGEFAFENPPAGDYELKISANGFSEQSQKITVGDRSQTLN
                     VRLTIGSPTVSVTAEINQEVDRVNLPQAVNLIGSQEIMERANPIIAQAAQEEAGINLQ
                     RTSPTMGGIFVRGLVGAKVNVYVDGVRYTTSAQRGGVSTFFNLNEPTGLQSIEVLRGS
                     NGAQYGSDALGGTVSLLTRVPMLGDTTPEFHGEFNTGFNSADRSFGGSTLLSYGTNRL
                     GGYVNLAARRVNNLRVARGIDTHSALTRFLGLPSDILGERLPGTAFTQYGGAFRLNYS
                     PTKDSQLIFNYQRSQQDGGKRYDQMLGGDGNLIADLRNLMLDFGYLRYVKTDFLGFNS
                     ASFAVSFNSQREERVNQGGQGDPFGAITHQYERTTATGFSFFLDKNLPARNSFLFGGD
                     IYSEKVNAPAYTYNPRTNIFALSRPRVPDEAKYLLGGLFVQDAWQAIPNRLRISGALR
                     YNVANYKVRGSDSPVVGGLRLWNDDSLSVGDFSGRIGAVLRVYDELRLAVNYSRGFRT
                     PSITDLGTLGLTGDGFEVDYTAANNLGGTIGTTAAATAVSTGLPVAKQRSEITDSYDL
                     GLRYGNKRFETEATVFLIDLKDAITKQALILPQGAVGQFLGDQQITSQLPNGVVFVPL
                     STSPVLVRANYTGARIYGFEFEAEAKITKELKFTGNYTYIHAKDTLTGLPPNIEGGTP
                     PATGFVSFRYEPLGRPFWFEFYSTIAAKQDRLSSLDLSDRRTGATRTRGQIQNFFRRG
                     ACVQGLTNNPDGICGSGDETILLPTGETLTQVQNRVLGVGVNSAPLFTYLPSYAIMNL
                     RGAFRLRETHQFFWEFENIFDRPYRNPSWGIDGAGRSLRVAYRFRF"
     gene            complement(2735..3367)
                     /locus_tag="VGB68_15480"
     CDS             complement(2735..3367)
                     /locus_tag="VGB68_15480"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY0050694.1"
                     /translation="MSHIVLLGDSIFDNKSYVGAEPDVVSHLRQMIPADWRATLNAVD
                     GSVVETVGAQLSKIPVEATHLIISVGGNNALMNADVLRMPAGSAAEVLNALAERASDF
                     EFQYRQMLSNILRRDLPTAVSTIYYPNFPDSLIQKIATTALTVFNDVIIRQAISAGVP
                     FLDLRLICNETSDYANEIEPSGKGGAKIAAKILEVVETHNFSNHRTCVYF"
     gene            complement(3548..3976)
                     /gene="rsfS"
                     /locus_tag="VGB68_15485"
     CDS             complement(3548..3976)
                     /gene="rsfS"
                     /locus_tag="VGB68_15485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEY0050695.1"
                     /translation="MENTLKEKSLQHETATVTITESRTPFSELDENIRLALACAADKK
                     AVNTIVLDLREIASFTEFFVITGGTNQRQVQAIADEIQEQLKKQLNSRPIRIEGYNTA
                     EWILLDYGDFIAHVFDQKSREFYDLERLWRDARKVEIPNE"
     gene            complement(3986..4645)
                     /gene="nadD"
                     /locus_tag="VGB68_15490"
     CDS             complement(3986..4645)
                     /gene="nadD"
                     /locus_tag="VGB68_15490"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEY0050696.1"
                     /translation="MKRIAFYGGSFDPVHDGHLTIARKLSELFEIDEFVFIPAFHAPH
                     KKDKKPTSAFHRFAMLCLATSDEPQIKVSKMEIEAPERPFTVETLTRLKNELTDTKIF
                     FVMGADSWQDITTWREWEKVLTMTNIIVVTRPDYEIVFSHVTEEIRERIVDLRNRRQT
                     VRDSRLTIDDSRIFITDAVRIDVSATEIRRKIRDGDRSWQKLLPAEVIKHIEKYGLYL
                     K"
     gene            complement(4645..4959)
                     /locus_tag="VGB68_15495"
     CDS             complement(4645..4959)
                     /locus_tag="VGB68_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050697.1"
                     /translation="MPNEEAKKMNAEQRDKWNKTRQKGQFRFILNGFFLYGLAGAFLF
                     ILNDYVFEFFFRAAPRYLHESDRFANKILFHLVVFSLIGIYVKNSLWNEKEEEFFRNP
                     EE"
     gene            complement(4960..5100)
                     /locus_tag="VGB68_15500"
     CDS             complement(4960..5100)
                     /locus_tag="VGB68_15500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050698.1"
                     /translation="MDFSFRGYDFAIDRRLGDFRFLAENQDCSKEILLEVCDHFRQLS
                     EK"
     gene            complement(5183..6226)
                     /gene="obgE"
                     /locus_tag="VGB68_15505"
     CDS             complement(5183..6226)
                     /gene="obgE"
                     /locus_tag="VGB68_15505"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEY0050699.1"
                     /translation="MFIDRAKIKIKAGDGGNGVTAMRREKFVPRGGPSGGDGGRGGSV
                     WLEADEGLNTLLHLRYNPEHKAERGRHGEGSNRHGKDGEDITVKVPVGTQVFDTESGE
                     IIFDFTEAGQRFLAAKGGKGGWGNARFATPTRRAPKFHYTGRPGEEKELQLELKLIAD
                     VGLVGFPNAGKSTLISVISAAKPKIADYPFTTLEPNLGVVDLGDYKTFIVADIPGLIE
                     GASEGAGLGDRFLRHVERTKLILHLVDVSSISGRNPVEDYEIINRELANYNQDLAQRP
                     QIIVATKIDALDEPERLENLRERARADDKDFLEISSVTNQGTKELVSAVAKMLGEISA
                     AGLELKIELLNED"
     gene            complement(7350..7637)
                     /gene="rpmA"
                     /locus_tag="VGB68_15510"
     CDS             complement(7350..7637)
                     /gene="rpmA"
                     /locus_tag="VGB68_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEY0050700.1"
                     /translation="MAHKKGVGSSRNGRDSAAQRLGLKKFGGEKVRGGNILARQRGTK
                     WKPGNNVGRGKDDTLFALIDGIVTFENKGQKGKFISVYAEAATAEVESQAA"
     gene            complement(7726..8016)
                     /gene="rplU"
                     /locus_tag="VGB68_15515"
     CDS             complement(7726..8016)
                     /gene="rplU"
                     /locus_tag="VGB68_15515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEY0050701.1"
                     /translation="MSYAIIKTGGKQYPVEAGQTLRVPSIQGNEGESVDVETLLVSKD
                     GNTSTEGATLKATIVSHGKADKVIVFKKKRRKQYKRKQGHRQGYTEIKIDTI"
     gene            complement(8721..9209)
                     /locus_tag="VGB68_15520"
     CDS             complement(8721..9209)
                     /locus_tag="VGB68_15520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEY0050702.1"
                     /translation="MESRLKISKDEFRAALGRFASGVAVVTTKDNENRLHGITVSAFC
                     SVSLEPPLILVCIDKEAGSHRAFEQSRAFVVNILREDQQYLSDRFASHLRDKFEGVKY
                     RDGIDHLPVLEDVLVNLECRLVNAHDDGDHTIYIGEIVVSTVNDGNPLVYFKGVYRKL
                     GS"
     gene            9311..10189
                     /locus_tag="VGB68_15525"
     CDS             9311..10189
                     /locus_tag="VGB68_15525"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF045485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEY0050703.1"
                     /translation="MMELKEFFEASRKAVDETLDALIPNSEAEPKKLHAAIRWSVFAG
                     GKRFRPALLFAAGKTFGADIEKLLKTAAAIEMIHAYSLIHDDLPAMDDDDLRRGRETC
                     HIKFDEATAILAGDRLQSLAFQAIAEESNLPSEIRLRLVAELADSAAKMVDGQHFDLE
                     AEGKTISIKELKAIHQNKTGAMISAAARGGAIIAVAGEKELEAISDYASSLGLLFQIT
                     DDLLDVTQTTEVLGKTAGKDISAEKATYPAFYGIEETRKLAAEAHAEAIAALNRIDRN
                     TDLLRALVGYILQRQK"
     gene            complement(10451..11923)
                     /locus_tag="VGB68_15530"
     CDS             complement(10451..11923)
                     /locus_tag="VGB68_15530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050704.1"
                     /translation="MQSNYLIKFAVLVICLISSTAAFADIKIKTRQTAGGQTSENTTY
                     IKGKRQRSEMMDGKMINITQCDLRRDVQIMPDGKVYMVNPFGDAAAQEDKTVSQSQTT
                     TKLETGGTVTMTYTTKDTGERRQMFGYTAKHLIITMESVASPDACSKNNSKMVMDGWY
                     IDATWYFQCDRGYANGYTPKSQKAGCQDKYLMKQVGTANRGYPVLEKMTMYDETGREM
                     MTSLSEVVELSQATLEASLFDVPQGFREVKNSSELYASMSSQSSSQSSYSSTTSSKNG
                     GDYDAPSNSGMSRNVKNMSKSDESVSSTVGAKKEGTVRIGLAGVKTGAIGEGLNATEL
                     AGAIQSTLGEYLKGTKIELVQIEARLPTAIDAEAKQKECDYVLYAQVSHKKGGGGFGM
                     FSKIAPALGSVVPVAGMGSRAGQVAGQVANTTIYTAANAAGNVKSKDELTLEIKLNAA
                     GGGAAALAKQYKAKAKSDGEDIISPLVEQAAAAILETVGK"
     gene            complement(12173..14062)
                     /locus_tag="VGB68_15535"
     CDS             complement(12173..14062)
                     /locus_tag="VGB68_15535"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEY0050705.1"
                     /translation="MKKLVFLTVLFLSIFSLEAFSAVKTWDGGGADANWQTAANWVGD
                     VAPAANDDLVFPATAAQQATNNNLFFFIAFNSLTFEGGAYTVSGNPITLRGLNAVAGT
                     HSINTAVSVSGAQTFNAGQAAVMTIVILNVGGNPLTLAGAGSFGIGLLSGSGAITKNG
                     IGASLIAGSSGYSGALNINDGILVVDANLPNSVVTINSSSTGGAFGISGLGGTGTVGA
                     TTVTNGGISAGTLDSPTGILTVNGNLNFAPTGNFVAKIGGTTPGANGHDQLNVNGAVT
                     LNNARLTPLPWNAFRPAINDSFVILRNDGTDAINGTFLNAPEGAVFGGALNTAFRITY
                     QGGDGNDIVITRINKAGFDFDGDGKSDVSVFRPSDGTWYALLSGSGALFGRQFGASGD
                     QPAPADYDGDNKTDTAVFRPANGYWYVLRSADLTFTATQFGANGDYAVPNDFDGDGRA
                     DVAVFRPSDGGWYQLRSLTNQFVAQQFGNSQDKPVMGDFDGDGIGDLAVFRNDGNWYI
                     LRSSDGAFYGLNFGFGTDKICPADYDGDGRTDIAVFRGSNNPSEADFYFLPSGDPGQL
                     RGVPFGAAGDLPVVADYDGDGKSDFAVFRPGENAWYLLRSTAGFTGVVFGLAGDKPVP
                     AAFIP"
     gene            14268..15167
                     /gene="accD"
                     /locus_tag="VGB68_15540"
     CDS             14268..15167
                     /gene="accD"
                     /locus_tag="VGB68_15540"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEY0050706.1"
                     /translation="MSWFSRKKPKLEEQPADEDRKVKTEGIFVKCLECDTALYKGELE
                     EAQQVCKECGYHFRLGARERLKSLFDDEQYEKLDEDVTSTDPLEFVDTKPYKDRLKAA
                     KAASGLPEAIVSGKGRVGGHPVYAGAMDMSFIGGSMGSAVGEKITRLIERAIKTRGAV
                     IIFSASGGARMQEGTLSLMQMAKISSALSQLHAAQLPFISVLTDPTTGGVTASFAMLG
                     DIIIAEPKALIGFAGPRVIEQTIRQKLPKGFQRSEFLLEHGMVDMVVDRREIRGAIIR
                     LLNFMMNAEIKPKEDFFVSGSNP"
     gene            15199..15642
                     /locus_tag="VGB68_15545"
     CDS             15199..15642
                     /locus_tag="VGB68_15545"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HEY0050707.1"
                     /translation="MLKEALLEIFERDLDKLANEINLYADEDKLWITGGEISNSAGNL
                     CLHLLGNLNHFVGAVLGENGYVRNRESEFGSKNVTRQDLLAGIEKTKSAVAQTLNKLS
                     DESFEQNFPVEKHDKTVKTDFMLLHLLAHFNYHLGQINYHRRLTA"
     gene            15655..16974
                     /locus_tag="VGB68_15550"
     CDS             15655..16974
                     /locus_tag="VGB68_15550"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEY0050708.1"
                     /translation="MNFAESLSYLLSLGNEVLAMKLGLASTEKLLAALGNPQNNFLKI
                     QVAGTNGKGSTCAFLDSICLGANIKTGLYTSPHLVSITERIKLGGEEIGEEDFARHAT
                     RVREISENLVAKGELETVPTFFEQVTAIAVSAFAEAKIELAILETGLGGRFDSVTATR
                     AEIAALTPIDYDHEKILGGTLSEIASEKAAIIRERMKIVVSPQKAEAMRVILERCVEA
                     RVSPLLNGCEYFPDDCGEVGFKTPNEIYPRVRIGLEGEHQYENACLAINLAEILSMDF
                     GFSISGGEIYEGLGKARHKGRLEWWNHTRRNILFDGAHNIAGARALRNYLQKYCPESK
                     IAFVFGAMRDKNLSAIAEILFPLAEDLILTEPDNARCEKVEELEKYAERVIDKSKIFL
                     VPDVFDAVDSAIRIALKYPAESNSFVCVTGSLYLVGEAQKALTESVL"
     gene            17016..17579
                     /locus_tag="VGB68_15555"
     CDS             17016..17579
                     /locus_tag="VGB68_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050709.1"
                     /translation="MKRKVLWSFLSLILFASVNAQAQQKQVMTAANKIVSALKNKDMK
                     TVATFVHPAKGLRFAPYSYFGDSNLTFKKAQVPSLFLLRRTYLWGEFDGSGDPINLGF
                     PAYYKQFVYDRNFAKAPKISYEKRLGCGNGVFNVREVYPKAKFVEYHFPKGDDGNPAG
                     WNSLYLVFEKSGAIWYLAAIAHDEWTI"
     gene            17707..18465
                     /gene="gpmA"
                     /locus_tag="VGB68_15560"
     CDS             17707..18465
                     /gene="gpmA"
                     /locus_tag="VGB68_15560"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF010713.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEY0050710.1"
                     /translation="MHKLVLIRHGESEWNRENRFTGWKDVDLSAKGREEAKAAGQLLK
                     AEGFTFDEAYTSVLKRAIRTLWIILDEMDLMWIPETKTWLLNERHYGGLQGLNKAETA
                     AQYGDEQVLIWRRSYDTLPTELLETDERWLGKDPRYSGIEAGKFPKAEALKQTVERVV
                     PYFQTEIMPKVQAGRRLIIAAHGNSLRALVKYLNAISDEDIVQHNIPTGIPLVFEFDE
                     NFKPVNSYYLGDQEAIRAAQEAVASQGKAKYDKA"
     gene            complement(18555..19346)
                     /locus_tag="VGB68_15565"
     CDS             complement(18555..19346)
                     /locus_tag="VGB68_15565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050711.1"
                     /translation="MIRFSQLLTVFALLFGAAFFSFAQANKPDEKAEAILAKAVQKLG
                     GEKYLQIKSVVGSGNFSIYREGQSQITQSFIDIIVFPDRERTEFKTGGTRTIQTNVGE
                     TGWLADTSAKVIKEQDKDQIEGFKRSMRTSLDNLLRGSWRRDAALSFVGRREASLGKR
                     NNVVRLTYPDNFTVEFEFSDEGLPMKSVYKRTNAEGAEVREEDRYAQFIDVQGIYAPF
                     IIDHFQNGAQTSRINYLKIEYNKIIPDSIFNKPADAKELKKDLKF"
     gene            complement(19339..20064)
                     /locus_tag="VGB68_15570"
     CDS             complement(19339..20064)
                     /locus_tag="VGB68_15570"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY0050712.1"
                     /translation="MHKLLSTSLFLICACVSGFAQDAQPKPTATPGNGIGSGNGGGEI
                     KSSGSSKSANVRGVRILSKPRANYTQAARMNDIEGYVRLRITFLASGEIGDVIPITGL
                     PYDLTEQAIAAARQIKFEPQMKDGIPVTVSKIVEYGFWIYYRENDKDLAKNASILKMP
                     APEHPQKSDFRKIGGKVQVKVVFDTDGSLRVTEVSSDLPKDFQDAARKAASQIKFDPA
                     IHKNGNVVAQSKVIEYEFKPQND"
     gene            complement(20162..>20357)
                     /locus_tag="VGB68_15575"
     CDS             complement(20162..>20357)
                     /locus_tag="VGB68_15575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050713.1"
                     /translation="NRFAQTAPTMIFSLLQQLQALGLNVPDVLAQLGVNQDGKSANGS
                     EKTEIKLVEEIKPIEDGNGR"
BASE COUNT         4854 a         5205 c         5230 g         5068 t
ORIGIN      
        1 ttttcacttt agaaacgaaa gcgataagca actctcaagc tgcgtcccgc gccgtcgatt
       61 ccccagctcg gattgcgata agggcggtcg aagatgtttt caaactccca gaaaaactga
      121 tgagtttcgc gcaatctgaa agcgccgcgc agattcataa tcgcgtatga aggcagatac
      181 gtgaaaagcg gcgcggaatt aacgccgacg cccagaacgc ggttctgcac ctgcgtcaga
      241 gtttcgcccg tcggcagcag aatcgtttca tcgcctgagc cgcagatgcc gtccggattg
      301 ttggtcaagc cctgaacgca cgcgccgcgc cggaaaaagt tctggatttg cccgcgcgtg
      361 cgggtcgcgc ccgttcgcct gtccgataaa tccaggctcg aaagacggtc ttgtttagcg
      421 gcgatagtcg aataaaattc aaaccagaac ggtctgccca gaggctcgta gcgaaagctg
      481 acaaagccgg tcgccggcgg cgttccgcct tcgatgttgg gcggcaagcc ggtcagcgtg
      541 tctttggcgt gaatatacgt gtagtttccg gtaaatttta attccttcgt tattttcgct
      601 tccgcctcga attcaaaacc gtaaattcgc gcgcccgtgt aatttgcgcg aaccagaacg
      661 ggcgaggtcg aaagcggcac gaaaacaaca ccgttcggca actggctggt aatttgctgg
      721 tcgccgagga attgcccgac cgcgccctgc ggcagaatca aagcctgttt ggtgatggcg
      781 tctttcaggt caatcagaaa aacggtcgct tcggtttcaa aacgcttatt accgtagcgc
      841 agacccaaat catagctgtc ggtgatttcc gagcgctgtt tggcgacggg caagccggtc
      901 gaaacggcag ttgcggcagc cgtcgtgccg attgtgccgc ccagattgtt tgccgccgtg
      961 taatcgactt caaaaccgtc gccggtcaag ccgagagttc ccaaatcggt gatgctcggc
     1021 gtgcgaaaac cgcggctgta atttaccgcc aagcgcagtt catcgtaaac tctgagaacc
     1081 gcgccgatgc gccccgaaaa atcgccgacg ctcaaactgt cgtcgttcca caatctcaaa
     1141 ccgccgacaa cggggctatc agagccgcga actttataat tcgcgacgtt ataccgcagc
     1201 gcgccgctga ttctcaaacg gtttggaatc gcctgccacg catcctgaac gaacaaaccg
     1261 cccaaaagat attttgcctc gtccggcacg cgcgggcgcg aaagcgcgaa aatattcgtg
     1321 cgcggattat aggtgtaagc cggcgcgttg accttttcgc tgtagatgtc gccgccgaat
     1381 aaaaaggaat tgcgcgcagg caggttttta tcgaggaaaa agctgaagcc ggtcgccgtc
     1441 gtccgctcgt attgatgtgt gattgcgccg aacgggtcgc cttgaccgcc ctgattgacg
     1501 cgctcttcgc gctggctgtt aaagctgacg gcaaacgacg cgctgttaaa accgagaaaa
     1561 tccgtcttca cgtagcgcag atagccgaaa tcgagcatta aatttctcaa atcggcaatc
     1621 aggttgccgt cgccgccgag catctggtcg taacgcttgc cgccgtcctg ctggctcctt
     1681 tgataattga aaataagctg gctgtctttg gtcggcgagt aattcagacg aaacgcgccg
     1741 ccgtattgcg taaaagccgt gccgggcaga cgctcgccga gaatgtcgga cggcaagccc
     1801 agaaatctgg tcagagccga gtgcgtgtcg attccgcgcg ccacgcgcag attattaacg
     1861 cgccgggcgg cgagattaac gtaaccgccg agtctattcg tgccgtagct gagcaaagtc
     1921 gagccgccga aagatcggtc ggcgctgttg aaaccggtgt tgaattcgcc gtgaaattcg
     1981 ggcgtcgtat cgccgagcat cgggactctg gtgagcaggc tgaccgtgcc gccgagcgcg
     2041 tccgagccgt actgcgcgcc gttcgagccg cgcaatactt cgattgactg taagcccgtc
     2101 ggctcgttca gattaaaaaa cgtgctgacg ccgccgcgct gcgccgaggt cgtgtagcgc
     2161 acgccgtcca cataaacgtt gactttcgcg ccgaccaaac cgcgcacgaa aattccgccc
     2221 atcgtcggac tcgtgcgctg caaatttatt ccggcttctt cctgcgccgc ctgcgcgata
     2281 atcgggtttg cgcgctccat aatttcctgg ctgccgataa gattgacggc ttgcggaaga
     2341 ttcacgcggt cgacttcctg attgatttcc gcagttacgg aaacggtcgg cgagccgatc
     2401 gtcaggcgca cgttaagcgt ctggctccgg tcgccgaccg tgattttctg cgattgctcg
     2461 ctaaatccgt tggcggaaat ctttaattca taatcgcccg ccggaggatt ttcaaaggcg
     2521 aattcgcccc gctcgttggt cacgacggca cgcgaaatcg tgccgcgcag aatcacctgc
     2581 gcgccgacga tgacggcgtt attctggtcg gtgacagtgc cggaaattcg agttgtcgtt
     2641 tgagcgtgag ttgcggagaa aaggctgaag acgccaagca aaagcgtcat caacagtttg
     2701 gtcgcgttaa ttttcattat ttcttcctat tacattaaaa ataaacacaa gttctatggt
     2761 ttgaaaaatt gtgggtttcg acgacttcca gaatcttagc ggcgattttc gctccgcctt
     2821 tgccggacgg ctcgatttcg ttggcgtaat cgctcgtttc attacaaatc aagcgtaaat
     2881 ccagaaacgg aacgcccgcc gaaatcgcct gtcgaataat aacgtcgtta aaaaccgtca
     2941 gcgcggtcgt cgcgattttt tgaatcaaag aatcgggaaa gttaggataa tatatcgtag
     3001 aaacggcggt cggtaaatct cggcgcaaaa tattcgaaag catttggcga tattgaaatt
     3061 cgaaatcgct cgcccgttct gccagagcgt ttaaaacttc cgccgccgaa ccggctggca
     3121 tccgcaggac atcggcgttc atcagcgcat tgtttccgcc gacgctgata atcaggtgcg
     3181 tcgcttcgac gggaattttc gagagctgcg cgccgaccgt ttcgaccacg ctgccgtcga
     3241 cggcattgag cgtcgcgcgc cagtccgccg gaatcatctg ccgcaggtgc gagactacgt
     3301 caggctccgc gccgacatag gacttgttgt cgaatattga atctcccagt aaaacaatgt
     3361 gagacataaa aatttattgc cggagctgcg ggttaaatgc cgccgagatt acttgcagaa
     3421 aacgcttcgg aatagataag agatgaaaga tgagagccga tagttttccg tttttatctt
     3481 tcatctatcg ggaattatct gttccgaagc gtttttctat cgccacgaat ccccaaacat
     3541 tatcctttta ttcattggga atctcaactt ttcgcgcgtc gcgccagagc cgttccaaat
     3601 cgtaaaactc gcgcgatttc tggtcgaaga cgtgcgcgat aaaatcgccg taatcgagca
     3661 gaatccattc cgccgtgttg tagccttcga tgcggattgg acgggaattt aattgttttt
     3721 tgagctgctc ctggatttcg tcggcaatcg cctgcacctg tctttgattc gtgccgccgg
     3781 taatgacgaa aaattccgta aaactggcta tttcgcgcaa atccagaacg attgtattga
     3841 cggctttttt gtcggcggcg caggcgagcg cgagccggat attttcgtcg agctccgaaa
     3901 aaggcgtgcg cgattcggta atcgtaacgg ttgcggtttc gtgctgcaaa gatttttctt
     3961 taagcgtatt ttccatttct gtagattatt tcaaatatag accgtatttt tcaatatgtt
     4021 ttatgacttc cgccggcaga agcttttgcc agctccggtc gccgtcacga attttccggc
     4081 gaatttccgt cgccgaaacg tcaatccgga cggcatcggt tataaaaatt cgtgaatcgt
     4141 caatcgtcaa tcgcgaatcg cggacggtct gccgacggtt tcgcaaatcg acgattcttt
     4201 cccgaatttc ttcggtcacg tgcgaaaaaa cgatttcata atcgggacgc gtgacgacga
     4261 taatattcgt cattgtcaaa actttttccc attcgcgcca cgttgttata tcttgccacg
     4321 aatcggctcc catgacgaag aaaattttcg tgtcggtcag ctcatttttc aggcgcgtca
     4381 gcgtttcgac cgtaaaaggg cgttcgggcg cttcgatttc cattttggaa actttgattt
     4441 gcggctcgtc gctcgtcgcc agacaaagca tcgcgaaacg atgaaaagcg gaagtcggct
     4501 ttttatcttt tttatgcggc gcgtgaaaag cgggaatgaa aacgaattcg tcaatctcga
     4561 aaagctccga cagctttcgc gcaatcgtca gatgcccgtc gtgaaccggg tcgaagctcc
     4621 cgccgtaaaa tgcgattcgt ttcattattc ttccggattt cgaaaaaatt cctcttcctt
     4681 ttcattccac agagagtttt ttacataaat gccgataagc gagaaaacaa ccagatgaaa
     4741 caagattttg ttagcgaacc tatccgattc gtgcagatat ctcggcgcgg ctctaaagaa
     4801 aaactcaaaa acataatcat tcagaatgaa taaaaatgct cctgccaatc cataaagaaa
     4861 aaaaccgttt aaaataaagc gaaattgtcc tttttgacgt gtcttattcc atttgtctct
     4921 ttgttcggca ttcatttttt ttgcctcttc gttcggcatt catttttccg aaagctgccg
     4981 gaaatggtcg cacacttcaa gtaaaatctc tttggagcag tcctgatttt ccgcgagaaa
     5041 ccggaaatcg cccagccgcc tgtcgattgc aaaatcatag ccgcgaaatg aaaaatccat
     5101 ccgaactccc gtaaaatcag cttatttctc cgggcaaatc cgcaaattcg gcgagccgcc
     5161 cggcgtcaga aagttaataa gctcaatctt cattcagaag ctcgattttc aattcgagcc
     5221 cggcggcgga aatttctccc agcattttcg ccaccgccga gaccagttcc ttcgtgccct
     5281 gattcgtcac cgaggaaatt tccagaaagt ctttgtcgtc tgcgcgcgcc cgctcgcgca
     5341 gattttccag tctttccggc tcgtccagcg cgtcgatttt cgtggcgacg ataatctgcg
     5401 ggcgctgcgc caaatcctga ttgtaatttg ccagctcgcg attgatgatt tcgtaatctt
     5461 ccaccggatt tctgcccgaa atcgaggaaa catcgacaag atgcaaaatc aatttcgttc
     5521 tttcgacgtg ccgcagaaaa cggtcgccga gccccgcgcc ttccgacgcg ccttcgatca
     5581 aaccgggaat atcggcgacg atgaaagttt tgtaatcgcc cagatcgacg acgccgagat
     5641 tcggctccag cgtggtgaaa ggataatcgg caattttcgg cttggcggcg gaaatcaccg
     5701 aaatcaaagt cgatttcccg gcgttcggaa aaccgaccag cccgacatcg gcaatcaatt
     5761 tcaattcgag ctgtaattcc ttttcctcgc ccggtctgcc cgtgtaatga aacttcggcg
     5821 cgcggcgcgt cggcgtggca aaacgcgcgt ttccccaacc gcctttgccg ccttttgccg
     5881 ccaggaaacg ctgtccggct tcggtaaaat cgaaaataat ctcgcccgat tccgtgtcga
     5941 aaacctgcgt tccgaccgga actttaacgg tgatgtcttc gccgtccttg ccgtgccggt
     6001 tcgagccctc gccgtgtcgc ccgcgctcgg ctttgtgttc gggattatag cgcaggtgca
     6061 gaagcgtatt taaaccttcg tcagcttcca gccagacgct gccgcctctg ccgccgtcgc
     6121 cgccggaagg tccgccgcgc ggcacgaatt tttcgcgacg catagccgtc acgccgtttc
     6181 cgccgtcgcc ggctttgatt ttaatttttg cgcggtcgat aaacatatat aactttagat
     6241 tttagagttg cgggttgcga ttggcaaacg aggcggaaac ccggacgtgc tttaaggacg
     6301 gaaacatctt agggaaatga gaggctgatg aatcgggatt agataagatt gattaagata
     6361 aaatcctgat tcattccaaa taatcgtatt aaatcagtgc cgtatttctt tgaaaaaaag
     6421 aaagcgctgt cttaagaaat taagacagcg ctttctttat ccttaaagaa taccgaagta
     6481 tatcggataa ggttggcgat tgtcacatgt acacgcttca atcgccgatt ttgtattccg
     6541 aagagcctcg cggcgagcgc atctccggtc agcacacgaa atgtttgcac atcccgctcg
     6601 cgcccttaaa gcgcagtgcc gttaggtttt tgttgtggtg gcttttcctc caccgccggt
     6661 tacgtccccg gcaacgaggc tcacttatcg ctttcgcgcg tgagcggcat tgctcccttt
     6721 cggctgttcc tttccaaaag gcgttgtccg tttttatccg gacaactcga aaagtcttgc
     6781 gacctttctt gagccaagcc tttcggcttc gattcagggc gtaatagcag ctaatttccg
     6841 tctttcactg ctctcggaca atgcgtcgcc gccttcgtcc gcctctccct gtatcggtca
     6901 aacgcccata agcaatctga ctgtaaaatg gattctacac cagttttttt tcctgtcaag
     6961 aagaaaatac tttaagtttt gccgctgttt tacgagtcga tttcgagcag gctgtgggaa
     7021 atttcagcct cagcctgtgg aaaaaaatgt ggaaaacttc gcaagccgtt tgagtttaaa
     7081 atttaaaaat ccgggcgatt ttcccgagct gcaaaatcgc ttgtggaaaa ttacggaaac
     7141 tgacacgtaa aaattttttt ccaaagtaaa aaaaatgatt ggggcgacgg atttaaatga
     7201 atttttgtcg aaaacagctt caaaattaaa attgccgccg aaattttccg aaattctttc
     7261 cgaaaaccgg cgtaaaaaga tgtcgatact cttaagaata tcgacatctt tttaaaacta
     7321 aagccggaaa caatctgaag ctcgaaagcc tatgcggctt gcgattcgac ttccgccgtc
     7381 gcggcttcgg cgtaaacgct gatgaatttg cctttttgac ctttgttttc aaaggtgacg
     7441 atgccgtcaa tcaaagcaaa aagcgtgtcg tcttttccgc gcccgacatt gttgcccggt
     7501 ttccatttcg ttccgcgctg gcgagccaga atgtttccgc cgcgcacttt ctcaccgccg
     7561 aattttttga gtccgaggcg ctgtgcggct gagtcgcgcc cgtttctcga tgaacctaca
     7621 ccttttttat gtgccataat tttattcggg attcaaaatt caaaattcag gattgcggaa
     7681 taatagcgca aatcctaaat cctaaatcct aaatcctaaa tcctcctaaa tcgtgtcaat
     7741 tttgatttcc gtgtaacctt gacggtgacc ttgcttgcgt ttgtattgtt tgcggcgttt
     7801 ctttttgaaa acaataactt tatcggcttt gccgtgcgaa acgatggtcg ctttgagcgt
     7861 cgcgccttcg gtcgaagtat tgccgtcttt gctgactaaa agcgtttcga catccacgct
     7921 ttcgccttcg tttccctgaa tcgaaggaac gcgtaaagtc tgtccggctt cgaccggata
     7981 ttgttttccg cctgttttga tgattgcgta actcattgtg atttatctcc ctcgcataag
     8041 acaagatttt gcttgtcagt cgaaaagaac aaattatacg aaatcaaacc gccaaaggtc
     8101 aaatcgaggc gaaagagagc ggcggaaaaa gcgcaggaaa tgagccgaaa gtgaaaaatc
     8161 cggctttctg ctcaatcgct caaaatccga aatactatca aaattgatag acagcctctg
     8221 aaaatactat caattttgat agtatttcgg attttcggag aatctccagt ttctttgtaa
     8281 cattagctac ttcaatactt agagaaatat ttcgccggtt cgcatttatg gcacgagaat
     8341 tggtatataa gactgcgttg tcggttatca cggatgattc ttcgctgcct cctggcaatt
     8401 ttgctgacac cgaatcatcc gtgatttaac tttaggtaat cggtcgatga atataaagcg
     8461 gtgcggtcga aaggcaaatc gaaaaaccgg cacgctgtat tcgcagtttt caaatctcgg
     8521 caacggacaa taaatgactg gcacagccga cgagccgtcg ttttgatgaa aaggcttttg
     8581 ataattttaa atattcggtg gattcgccga ataccttgtc gctaacgacg aacaaccggg
     8641 caaaaatact cacttacgtc ggtttgcccg ctatctcaac gttagcgaca ttattttttt
     8701 aagccctaat cttttcggtt tcagcttcct agctttcgat aaacgccctt aaaataaacg
     8761 agcggattgc cgtcattgac ggtcgagacg acgatttcgc cgatgtaaat cgtgtggtcg
     8821 ccgtcgtcgt gagcgttgac cagacgacat tccagattga ccagaacatc ttctaaaacg
     8881 ggcaggtggt ctattccgtc gcgatatttc acgccctcga atttgtcgcg cagatgcgaa
     8941 gcgaaacggt cggaaagata ctgctggtct tcgcgcagaa tattgacgac aaacgcccgg
     9001 ctttgctcga aagcgcggtg acttccggct tccttgtcga tgcagaccag aatcagcggc
     9061 ggttccagcg agaccgagca aaacgcgctg accgttattc cgtgcaggcg attttcattg
     9121 tctttggtgg tgacgaccgc gacgccgctc gcaaagcgtc cgagcgcggc gcgaaattcg
     9181 tcttttgaaa ttttcagacg gctttccatt aaaatatttt taccacacgc gcgcgaaaga
     9241 tgcagaaaca ggaaaatgaa ttttcaaatt ttgcgctttt ccgctccggc aggctattct
     9301 ttttctgtcg atgatggaac taaaagaatt ttttgaagcg agccgaaaag cggtcgacga
     9361 aacgctcgac gcgcttattc caaactctga agccgagccg aaaaaattgc acgcggcgat
     9421 tcgctggagc gttttcgcgg gcggcaagcg ttttcgtccc gcgctgcttt tcgccgccgg
     9481 aaaaactttc ggcgcggaca tcgaaaagct tttaaaaacc gccgccgcga ttgagatgat
     9541 tcacgcttat tcgctgattc acgacgattt gcccgcgatg gacgatgacg atttgcggcg
     9601 cgggcgcgaa acctgtcaca taaaatttga cgaagcgacg gcgattctgg cgggcgaccg
     9661 cttgcagtcg ctggcttttc aggcgattgc cgaagaatcg aatttaccgt cggaaattcg
     9721 cctccgactg gtcgcggaat tggctgattc agccgcgaaa atggttgacg gtcagcattt
     9781 cgacctcgaa gccgaaggaa aaacaatttc gattaaagag ctcaaagcga ttcatcaaaa
     9841 caaaaccggc gcgatgattt cggcggcggc gcgcggcggc gcgattatcg ccgtcgccgg
     9901 cgagaaagag ctcgaagcga tttcagatta cgcttcgtcg ctcggtctgc tgtttcaaat
     9961 tactgacgat ttactggacg tgacgcagac gaccgaagtt ctgggaaaaa cggcgggcaa
    10021 agacatttcg gcggagaaag ccacgtatcc ggcgttttac ggaatcgaag aaacgcggaa
    10081 gctagccgcc gaagctcacg ccgaagccat cgcggcattg aaccggattg atagaaatac
    10141 ggatttgctg agagctttgg tcgggtatat tctgcagcgt caaaaataat tcaaacgtat
    10201 ctgacagcac tgtaaaaaaa gattagccgc aaagaacacg gagctgacag agaaaagacc
    10261 gtcatcgttt ctctttattt cctgattttt ttgcggctga aattctttcg ccgtccttca
    10321 tcgaacagtt acggcggcgt gcttcggctt ttattttcaa gacaattatt tatgcgggct
    10381 taaattaaaa aaagagccgg tcgagcgaag accgtctcat caaggaggtt tgtgatgaca
    10441 cctggctgat ttatttgccg accgtttcga gaatcgccgc ggcggcttgt tcgacgagcg
    10501 gggaaatgat gtcttcgccg tcggatttgg ctttcgcttt atattgtttg gcgagagccg
    10561 ccgcgccgcc gcccgccgcg ttaagcttga tttcgagcgt cagctcgtct ttcgatttga
    10621 cgttgccagc cgcgtttgcc gccgtgtaaa tcgtggtgtt ggcgacctgt ccggcgactt
    10681 gcccggcgcg gcttcccatt cccgccaccg gcacgacgct gccgagcgcg ggcgcgattt
    10741 tcgagaacat tccgaaaccg ccgccgcctt ttttgtgcga aacctgcgcg taaagaacgt
    10801 aatcgcattc cttctgtttg gcttccgcgt cgatagccgt cgggagcctg gcttcgattt
    10861 gaaccagctc aatcttcgtg cctttcagat attcgccgag cgtgctctga atcgctccgg
    10921 caagctccgt cgcatttaag ccctcgccga ttgctccggt tttgacgccc gccaagccga
    10981 tgcgaaccgt gccttctttt ttcgcgccga cggtgcttga aacgctctcg tcggatttcg
    11041 acatattttt tacattccgg ctcatacccg aattcgaagg agcatcataa tcgccgccgt
    11101 ttttgctgct cgtcgtgctg ctgtagctcg actggctgct cgattggctc gacatactcg
    11161 catacagctc ggacgaattc ttgacttccc ggaagccctg cggaacgtcg aacagcgaag
    11221 cttccagagt ggcttgcgaa agctcgacga cttcgctcag cgacgtcatc atttctctac
    11281 cggtctcgtc gtacatcgtc attttttcca gaaccgggta gccgcggttc gccgtgccga
    11341 cctgcttcat caggtatttg tcctgacagc cggctttctg gcttttcggc gtgtaaccgt
    11401 tggcatagcc gcggtcgcat tgaaaatacc acgtagcgtc gatataccag ccgtccatca
    11461 ccattttgct gttgtttttc gaacaggcat cgggcgaagc gaccgattcc atcgtgataa
    11521 tcagatgttt cgccgtgtag ccgaacatct gcctgcgctc gccggtgtcc ttggtcgtgt
    11581 aggtcatcgt gacggttccg ccggtttcga gcttggtcgt cgtctgcgat tgcgaaacgg
    11641 ttttgtcttc ctgcgccgcg gcgtcgccga acgggttgac catataaact ttgccgtccg
    11701 gcataatctg aacgtcgcgg cgcaaatcgc actgcgtgat gttgatcatc tttccgtcca
    11761 tcatctccga gcgctggcgc ttgcctttga tgtaggtcgt attttcggaa gtctgcccgc
    11821 cggctgtctg ccgcgtcttg attttaatgt cggcgaaagc agcggtcgaa gaaattaaac
    11881 agataactaa aactgcgaat ttaataagat aattgctttg cattttgtat ctcccttatg
    11941 gaaaagaatc gttctctacc agctaaagcg gaaaggaaat acaaaaaggt atcaggcggg
    12001 aataaaaatt ttcaggattc aggatttaag attcaggatt caggattcag gattcaggat
    12061 tcaggattca ggatttaaga ttcaggattt aagattaaga tttaagattc aggattgccg
    12121 gtagccgtcg agcaatcctg aatcctgaat cctgaatcct gaatccttat ccttacggga
    12181 tgaacgccgc cggaaccggt ttatcgcccg ccagcccgaa gacgacgccg gtaaatccgg
    12241 cggtgcttct cagcaaatac caggcgtttt cgcccggacg aaaaacggca aagtcggatt
    12301 tgccgtcgcc gtcgtagtcc gcgacgaccg gaaggtcgcc ggctgcgccg aagggaacgc
    12361 cgcgcagctg acccgggtcg ccgctcggca gaaaataaaa atcggcttcc gacggattgt
    12421 tcgagccgcg aaaaacggcg atgtcggttc tgccgtcgcc gtcataatcg gcgggacaaa
    12481 ttttgtccgt gccgaaaccg aaattcaagc cgtaaaaagc gccgtccgag cttcgcagga
    12541 tataccagtt tccgtcgttg cggaaaacgg ctaaatcgcc gatgccgtcg ccgtcgaaat
    12601 cgcccatcac gggcttgtcc tggctgttgc cgaattgctg cgcgacaaat tgattggtca
    12661 agcttcgcag ctgataccag ccgccgtccg aaggacgaaa aaccgcgaca tcggcgcgcc
    12721 cgtcgccgtc gaaatcgttc gggacggcgt aatcgccgtt tgcgccgaac tgcgtcgcgg
    12781 tgaaagtcaa atcagcgctt cgcaaaacat accagtagcc gttcgccggt cgaaaaaccg
    12841 ccgtgtcggt tttgttgtcg ccgtcgtaat cggcgggcgc gggctggtcg ccgctcgcgc
    12901 cgaactgccg accgaacagc gcgccgctgc cgctcaaaag cgcataccac gttccatccg
    12961 acgggcgaaa aaccgaaacg tcggatttgc cgtcgccgtc gaaatcgaaa ccggctttat
    13021 tgatacgcgt gatgacaata tcgttgccgt cgccgccctg atacgtgatg cgaaaagccg
    13081 tgttcagcgc gccgccgaaa accgcgcctt ccggagcgtt cagaaacgtc ccgtttatcg
    13141 cgtccgtgcc gtcgttgcgc aaaatcacga acgaatcgtt gattgccgga cgaaaagcgt
    13201 tccacggcaa aggcgtgagc cgcgcgttat tgagcgtgac cgctccgttg acgttcaact
    13261 ggtcgtgacc gttcgcgccg ggcgtcgtgc cgccgatttt cgctacaaaa tttcccgtcg
    13321 gggcaaaatt taaattgccg ttgacggtca atattcccgt cggactatcg agagttccgg
    13381 cgctgattcc gccgttcgtc acggtcgtcg cgccgaccgt tcccgtgccg cccagaccgc
    13441 tgatgccgaa agcgccgccc gtcgagctcg aattaatcgt cacgacgctg ttgggcaaat
    13501 tcgcgtccac gaccagaatt ccgtcgttga tattaagcgc gccgctatag ccgctcgagc
    13561 ctgcaatcaa agacgcgccg attccgtttt tcgtaatcgc gcccgagccg gaaagcagac
    13621 cgatgccgaa gctgcccgcg ccggctaaag tcagcggatt gccgccgacg ttcaaaatca
    13681 caatcgtcat gacagccgcc tgcccggcgt taaaagtctg cgctccgctg accgagacag
    13741 ccgtgtttat cgagtgcgtt ccggcgacgg cgttcaagcc tcgcaatgtt atcgggtttc
    13801 cgctgaccgt atacgctccg ccttcaaaag tcagcgagtt aaaagcgata aagaaaaaca
    13861 gattgttgtt ggtcgcctgc tgcgccgccg tcgccggaaa aacaaggtcg tcgttggcgg
    13921 cgggcgcaac gtcgccgacc cagtttgcag ccgtctgcca gttggcgtcc gcgccgccgc
    13981 cgtcccacgt ttttacggcg gaaaaagctt cgagggaaaa aatcgagaga aataaaacgg
    14041 tcaaaaatac taattttttc ataaaacgag cctttggttt tgagatatcg gaaacttggc
    14101 tgaatttaac acaaaaaacg catccatcat aattataaat caccgataaa ggcttttccg
    14161 gctttctgag aagtttcggc gtgtggtaac cttgtgcaat gcaaaattta aaataaaata
    14221 gtcgtattca ttttgcgctt tgcattttga attttgcgtt aatttatatg tcctggttca
    14281 gtcgaaaaaa accaaaatta gaagaacagc ccgccgatga agaccgcaag gtaaaaaccg
    14341 agggcatctt tgtcaaatgc ctcgaatgcg acacggcgct ctataaaggc gagctggaag
    14401 aagcgcagca ggtttgcaag gaatgcggtt atcattttcg gctcggcgcg cgcgaacggc
    14461 tcaaaagtct tttcgacgac gagcaatacg aaaagctgga cgaagacgta acttcgaccg
    14521 acccgctcga atttgtcgat accaagccgt ataaagaccg cctcaaagcc gcgaaagccg
    14581 cttcgggctt gccggaagca atcgtttccg gcaaaggcag agtcggcggg catccggtct
    14641 atgcgggcgc gatggatatg agctttatcg gcggctcgat gggctcggcg gtcggcgaaa
    14701 aaattacacg cctgattgag cgcgcgataa aaacacgcgg cgcggtgata attttttcgg
    14761 cttcgggcgg cgcgcgaatg caggaaggaa ctttatcgct gatgcagatg gcgaaaattt
    14821 cctcggcgct ttcgcagctg cacgccgcgc agctgccgtt tatttccgtt ttgaccgacc
    14881 cgacgaccgg cggcgtgacg gcgagttttg ccatgctcgg cgacatcatt atcgccgagc
    14941 cgaaagcgct catcggcttt gccggaccgc gcgttatcga gcagacgatt cgccagaagc
    15001 tgccgaaagg ctttcaaaga agcgagtttc tgctcgaaca cggaatggtc gatatggtcg
    15061 tcgaccggcg cgagatacgc ggggcgatta tccggctgct gaattttatg atgaacgcgg
    15121 aaatcaagcc caaagaagat tttttcgtgt cgggcagcaa tccgtaaatg tttccaagat
    15181 aaaaaattcc tgaccgatat gttaaaagaa gcgctgctcg aaatattcga gagagatttg
    15241 gataaactgg caaacgagat taatttatac gccgacgaag ataaactgtg gataacgggc
    15301 ggcgaaatca gcaattcagc cggaaattta tgtctgcacc tgctcggaaa tctgaatcat
    15361 ttcgtcggcg cggttctggg cgagaacggt tatgtcagga accgggaaag cgagtttggc
    15421 tcgaagaacg tgacgcgtca ggatttgctg gcgggcatcg aaaaaacgaa aagcgcggtg
    15481 gcgcaaaccc tgaacaagtt atctgacgaa agcttcgagc aaaactttcc cgtcgaaaag
    15541 cacgataaaa cggtcaaaac ggattttatg ctgcttcacc tgctggcgca ttttaactat
    15601 catctcggac aaatcaatta tcaccgtcga ttgactgcct gacgctttag aaaagtgaat
    15661 tttgccgaat ctttatcgta tcttctctcg ctcggaaacg aagttctggc gatgaagctc
    15721 ggcttggctt cgaccgaaaa acttctcgcc gctctgggaa atccgcaaaa taatttttta
    15781 aaaattcagg ttgccggaac caacggcaaa ggctcgacct gcgcgttttt agattcgatt
    15841 tgcctcggcg caaatattaa aaccggactt tacacctcgc cgcatctggt ctccatcacg
    15901 gaaagaatca agctgggcgg cgaggaaatc ggcgaggaag attttgcgcg ccacgcgacg
    15961 cgcgtcaggg aaatctcgga aaatctggtc gcgaaaggcg agcttgaaac ggttcccacg
    16021 tttttcgagc aggtgacggc gattgccgtt tcggcttttg cggaagcgaa aatcgagctg
    16081 gcgattctgg aaacgggttt gggcgggcgt ttcgattcgg tcacggcgac gcgcgcggaa
    16141 atcgcggcgc tgacgccgat tgattacgac cacgagaaga ttctgggcgg cacgctgtcg
    16201 gaaatcgcgt cggagaaagc ggcgataatt cgcgaacgta tgaaaatcgt ggtttctccg
    16261 cagaaggcgg aagcgatgcg tgtgatttta gagcgctgcg tggaagcgcg cgtctcgccg
    16321 cttttaaacg gctgcgagta ttttccggac gactgcgggg aagtcggctt taaaactccg
    16381 aatgaaattt acccgcgcgt ccggattggc ttggagggcg agcaccagta tgaaaacgcc
    16441 tgtctcgcca tcaatctggc ggaaatttta agtatggatt tcggattttc catctccggc
    16501 ggcgagatat atgaaggact gggaaaagcg cggcacaaag gtcgcctgga atggtggaat
    16561 cacacgcggc gaaatatttt gtttgacggc gcgcacaaca tcgccggcgc gcgcgccttg
    16621 agaaattacc tgcaaaagta ttgtcctgaa agtaaaatcg cttttgtttt cggcgcgatg
    16681 cgcgacaaaa atttatccgc catcgccgag atattatttc cgctcgccga agacttaatt
    16741 ttaaccgagc ccgacaacgc gcgctgcgaa aaggtcgaag aattagaaaa atatgccgaa
    16801 agggtaattg ataaatcgaa aatctttctc gttcccgacg ttttcgacgc ggtcgattcg
    16861 gcaatccgaa tcgccctgaa gtatccggca gaaagcaatt cgttcgtctg cgtgaccggc
    16921 tcgctttatc tggtcggcga agcgcaaaaa gctttaactg aaagcgttct ttagaaggtg
    16981 agattttttt tcactttcaa acagggagaa attttatgaa aagaaaagtt ttatggtcgt
    17041 ttttatcatt gattttgttt gcttcggtta atgcgcaggc gcagcaaaaa caagttatga
    17101 cggcggcgaa taaaatcgtc agcgcgctga aaaacaaaga tatgaaaacg gtggcgactt
    17161 tcgttcaccc ggcaaagggc ttgcgtttcg cgccttacag ctattttggc gattcgaatt
    17221 taactttcaa aaaagctcag gttccgagct tgttcctgct tcgtcgaact tacctatggg
    17281 gcgagtttga cggttcgggc gacccgataa atctgggctt tccggcgtat tacaaacaat
    17341 tcgtctacga ccgcaacttt gcgaaagcgc cgaaaatttc ttacgagaaa cggctcggct
    17401 gcgggaacgg cgttttcaac gtgcgggaag tttacccgaa agcgaaattt gtcgaatatc
    17461 attttcccaa aggcgacgac ggcaatccgg cgggctggaa tagtttgtat ctggttttcg
    17521 agaaaagcgg cgcaatctgg tatctggcgg caatcgctca cgacgaatgg acgatttaaa
    17581 cgaacagcgt aattttttga aattgccttt aatcaaaaaa acagtttgac tcttcaaact
    17641 gttttttatt ttggctcggc ggtctgttaa aattgtcgtt cagactaaaa ttaaactttt
    17701 aaaattatgc acaaattggt tttaatcaga cacggcgaga gcgaatggaa cagggagaac
    17761 cgttttacgg gctggaagga cgtcgacctg tcggcaaagg gcagggaaga agcgaaagcg
    17821 gcgggacagc ttctgaaagc cgaaggcttt acgtttgacg aagcctacac ctcggtttta
    17881 aaacgcgcga tacgaacgct ctggattatt ctggacgaga tggatttgat gtggattccg
    17941 gaaacgaaaa cctggcttct aaacgagcgt cattacggcg ggctgcaagg cttgaacaaa
    18001 gccgaaaccg ccgcgcaata cggcgatgaa caggttttaa tctggcgcag aagttatgac
    18061 acgctgccga ccgagctgct cgaaaccgac gagcgatggc tcggcaaaga cccgcgatat
    18121 tcgggcattg aagcgggaaa atttccgaaa gccgaagcct taaaacaaac cgtcgagcgc
    18181 gtcgtgccgt attttcaaac cgagattatg ccgaaagtcc aagccggacg ccgcctgata
    18241 atcgccgcgc acggcaactc cctgcgcgcg ctggtgaaat atctgaacgc gatttcggac
    18301 gaagacattg tccagcacaa tattccgacc ggcattccgc tcgtttttga attcgacgag
    18361 aatttcaagc cggttaacag ttattatctc ggcgaccagg aagcgattcg agcggcgcag
    18421 gaagccgtcg ccagccaggg caaggcgaaa tacgataaag cctgatgaat cgataaacga
    18481 gaaacgcgga acgatgaatt tgatgtacag aatctgcaat tcatcgttcc gcgtttctcg
    18541 tttatcgttt gtctttagaa cttcaaatct ttcttgagtt ctttggcgtc cgccggttta
    18601 ttgaagattg agtcgggaat aattttgttg tattcgattt tcagataatt gatgcgcgag
    18661 gtctgcgcgc cgttttggaa atggtcgatg ataaacggcg cgtaaatgcc ctgcacgtcg
    18721 ataaactgtg cgtagcggtc ttcttcccgg acttccgcgc cttcagcgtt cgtgcgtttg
    18781 tagacggatt tcatcggcaa gccttcgtcg gaaaattcaa attcgaccgt gaaattatcg
    18841 ggataagtca atctcacgac gttgtttctt ttgccgaggc tcgcctcgcg gcgtccgaca
    18901 aagctgagcg ccgcgtccct gcgccaactg ccgcgcagca gattgtccag actcgtgcgc
    18961 atactgcgct taaagccttc aatctggtct ttgtcctgtt ccttgatgac cttggcggaa
    19021 gtgtctgcga gccagccggt ttcgccgacg ttggtttgaa tggttcgcgt gccgccggtt
    19081 ttaaattcgg tgcgttcgcg gtcggggaaa actataatgt cgataaagct ctgcgtgatt
    19141 tgcgattgac cttcgcggta aatcgaaaaa tttccgctgc cgacgacgga ttttatctgt
    19201 agatattttt cgccgccgag cttctggacg gctttcgcca gaatggcttc ggctttctcg
    19261 tccggtttat tcgcctgcgc gaaagaaaaa aacgccgcgc cgaatagtaa ggcaaaaact
    19321 gttaataatt gtgaaaatct aatcattttg cggtttaaat tcatattcaa ttacttttga
    19381 ctgagcgacg acgtttccgt ttttgtgaat cgcagggtca aattttattt gagacgcggc
    19441 ttttcgcgcg gcgtcctgaa aatcctttgg taaatcgctg gaaacttccg tgacccttaa
    19501 gcttccgtcc gtatcgaaga cgactttgac ctgcaccttg ccgccgattt ttctgaaatc
    19561 gcttttttgc gggtgctccg gcgcgggcat tttaagaatc gaggcgtttt ttgccaaatc
    19621 tttatcattt tcgcggtaat atatccagaa accgtattcg acaatcttcg acacggtaac
    19681 aggtattccg tctttcatct gcggctcgaa cttaatttga cgagccgccg caattgcctg
    19741 ctcggtcaaa tcgtaaggca gaccggtgat gggaatgaca tcgccgattt cgcccgacgc
    19801 taaaaatgtg atgcgcagcc ggacgtagcc ttcgatatca ttcatccttg ccgcctgggt
    19861 gtaattcgcg cgcggttttg aaagtatccg gacgccccgg acattggcgg atttcgaaga
    19921 gccggacgat tttatctcgc cgcccccatt tccgctgccg attccgtttc cgggcgtcgc
    19981 cgtcggtttt ggctgcgcgt cctgcgcaaa accggaaaca cacgcacaga ttaagaaaag
    20041 agaggtcgat aataatttat gcataaattt tctctggata agaaaggctt cagcgaaatt
    20101 ttatcaccga agcctttgat gcaaaagttt tgcgaaaagg ttgaatcgac cgtgattaaa
    20161 tttaccttcc gttgccgtct tcgattggtt ttatttcttc gaccagcttg atttcggttt
    20221 tttccgagcc gtttgcggat ttcccgtcct gattaactcc gagctgcgcg agcacgtcgg
    20281 gcacgtttaa tccgagcgcc tgaagctgtt gcagcagcga gaaaatcatc gtcggcgcgg
    20341 tctgcgcgaa acgattg
//
LOCUS       DASWNE010000799         7308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_27204, whole genome shotgun sequence.
ACCESSION   DASWNE010000799 DASWNE010000000
VERSION     DASWNE010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7308
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27204"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VGB68_15580"
     CDS             complement(<1..593)
                     /locus_tag="VGB68_15580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050714.1"
                     /translation="MKLSKFIIENMEAILTEWESFAETILPAAETMDSIALRDHAKQI
                     LEAVARDIETSQTAQEQSDKSKDLAPVPVGKETAATTHGALRHLAGFDLRQLGSEYRA
                     LRASVIRMWKARLTEIGDSEFNDMMRFNEAIDEALAASIVRYSDEVDRSRQTFLAILG
                     HDLRSPLQSILMAASYLLKSGLVEAKQLDVASRIKSSV"
     gene            952..1944
                     /locus_tag="VGB68_15585"
     CDS             952..1944
                     /locus_tag="VGB68_15585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY0050715.1"
                     /translation="MSATQRPKRDLRPALVFLSGDFLAVPIPLEREEVILGRALEADV
                     RVNDKKVSRQHARITTVQNGETEEPSYILTDLDSRNGIFLNGQRIKQETLQNGDKITI
                     GEHILRFEMLDEIDREYQRQIRRLLSHDDLTGLLSSRSFFSELRREASQPEAEKRQFC
                     VLMMDVDFFKNVNDTYGHLTGSKTLEEIGLCITQILRSGDVASRFGGEEFAAFLMDAE
                     LTQGIVAAERIRSTIEEQEFSVIRQGKPTESHHITISIGVASFPDDSSDPIELVEMAD
                     SALYRAKREGRNRVCAYRELSEEETKKAQLARREEHEPVSGNPDLTTRNQISEL"
     gene            2282..2914
                     /locus_tag="VGB68_15590"
     CDS             2282..2914
                     /locus_tag="VGB68_15590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050716.1"
                     /translation="MINKLNLATHPFRNRTLPYLIALACCTLAMVGVIFGFAKLRQIK
                     AESEIVESEVRLMETQLSELKGKGDQIQQTLTPEQRTLLIAAHKLVAQKSFGWSRLFA
                     DLEDVLPGGVSVSRINVENVYQTGDRTQAELQLSVLSRDYQGVMNMIDTMNSRPIFQA
                     ELRGQDLQKKEYFTYTEYTLRLIYTQPLGYSTAPAPANDVASVNEKGGQQ"
     gene            2920..3948
                     /locus_tag="VGB68_15595"
     CDS             2920..3948
                     /locus_tag="VGB68_15595"
                     /inference="COORDINATES: protein motif:HMM:NF022201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspMb/PilO family protein"
                     /protein_id="HEY0050717.1"
                     /translation="MSENLDPKDQPQQQAEIIRDDFTPQDSTVVVDEKDRTVILTEDE
                     TIVIEKQPTFDIAPKNRPRKVNLGIFGTTEYIVVGLGLLSIVATLLFYMFVVTPEQAK
                     LNNRKAERDQKEKELSTSKDKFADFTTTEEHVARLVGSVDDFESRFLPVSGNGRTALY
                     QRLNTLIAGYGLINTTGPDYAPLEINQREGQPEGEKGRAKYHSLFPGVYVTMTVEGSY
                     QNLRRFLRDLETSQQFIIVSTVELEPAENQDRQTDATTVTQTTQPSVTSPNQRFARPN
                     IDPTGAANPMMRNNPGQVTTTTTTTQQTTRPAPRGRTIGATVSLHIELAAYFRRNSAV
                     SILPPQQQ"
     gene            3962..5101
                     /locus_tag="VGB68_15600"
     CDS             3962..5101
                     /locus_tag="VGB68_15600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050718.1"
                     /translation="MNIFEGKTPSEKKKLIAAMVLGGLAVLTLVYNFSGLTGGRKTTV
                     TVSVSPTPSATISSSNGTQTSNIKLLSQDQINQEWLTSPVVYTPGSFYAPDAGRNIFA
                     FYEPPQPTPYEPVPVVETPLPTPPPTPVPTPLPILVSYVSPQSVYAGSRSFRLEVNGD
                     KFTPDSLIFWNGSQIPTSFVNTQKLTADISANLIAGEGQRSVEVRTPDGKLYSNPVMM
                     TVQAPPRPTVQYIGAEIARRGNNNTAYFREPGVPVEFGRRLSDIVNVNTVNNRFRVVS
                     ISVNEVILEDTSLGFRHRLPLARPSGAGGGTTGGGQTTPSRGGFDRNTTPGRFPNEGT
                     TVVPYNPTMPQTQEIPGIPTNIPRYVPPTPQQQKKDEEDDEDGDN"
     gene            5070..5852
                     /locus_tag="VGB68_15605"
     CDS             5070..5852
                     /locus_tag="VGB68_15605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050719.1"
                     /translation="MKKTMRTAIIKKAISHQRSAVSRRKRTPQSAIGNRQSGYSLVAL
                     MAMMTLLAITMMAVAPSVYQQVQREKELESIRRGEEVAEAIRQYVNHYNGAKFPSSMD
                     DLLEGIPYGTKKRQILRESAAIDPLSEDGKWRLVGVESKTMVNFARRVQNYNNGALPT
                     SENVHQSVINRLGRIITNTDANSEDDIKESADETDEDDGEVRTDNTPFIGVVSQSKNK
                     SFIAYYGIENHSKWVFTPLFRGTGSTPVGGSTSSKPRPVLQQ"
     gene            complement(5860..6765)
                     /gene="hslO"
                     /locus_tag="VGB68_15610"
     CDS             complement(5860..6765)
                     /gene="hslO"
                     /locus_tag="VGB68_15610"
                     /inference="COORDINATES: protein motif:HMM:NF001033.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEY0050720.1"
                     /translation="MDKLIHGTAAGGTIRVMAAITSDIVAEAVRRHQTSPTASAAFGR
                     VLTGTLLLGSSLKEFDRLTVKIDAQGAIEGIVAEANTEGKVRGYVRNPQAELPFKPDG
                     KFDVGGIVGGGMFHVIRESGFDIGLYREPYHGSVPIVSGEIAEDFAFYLAKSEQIPSA
                     VMLGVLLQNTEPFVKASGGVILQMMPGANEHIITMIEDTINHAPHLTTVINDETTPQD
                     LARLALGEIDFEVLEEKEIEFRCNCSFERAVSLIGSLGKAEVASMLAEDKGATMTCGF
                     CNETYSLNEADLKEILSSMENQEIL"
     gene            7125..>7308
                     /locus_tag="VGB68_15615"
     CDS             7125..>7308
                     /locus_tag="VGB68_15615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050721.1"
                     /translation="MSENKNLNHSILEIEQGGTTQFTKLLKICENFFGEYRINSSHYI
                     FKTPWQGDPRINLQKGK"
BASE COUNT         1901 a         1937 c         1873 g         1597 t
ORIGIN      
        1 acggagcttt tgatgcgcga ggcgacatct aattgtttag cttcgacgag tcctgatttc
       61 aaaagatagg aagccgccat taagatagat tgcagaggac ttcgcaggtc atgtccgaga
      121 atggctaaaa aggtttgtcg cgagcggtcg acttcatctg aatatcgtac aatcgaagcc
      181 gccagcgctt cgtcgatggc ttcgttaaac cgcatcatat cattaaactc ggaatcgccg
      241 atttccgtca gccgcgcctt ccacatcctg atgacgctcg ctcttaatgc ccggtattcg
      301 ctcccgagct gtcttaaatc aaatccggca agatggcgca gcgcgccgtg agttgttgcc
      361 gcggtttcct ttccaaccgg cacgggagcc aaatcttttg atttgtcgga ttgttcctgc
      421 gctgtctgac tggtctcgat gtccctggcg actgcctcca gaatctgttt ggcatggtcg
      481 cgtaaggcta tggaatccat tgtttccgcc gccggcagaa tcgtttcggc aaatgactcc
      541 cattcggtga gaatggcttc catattttcg ataataaatt tagaaagttt catttaatct
      601 gactatatca cttgttttac cagataacgc ttgataatta ccggcagagc gaaagcatta
      661 tttgctcgtg aatttcagta agcaaacagt tcggtatttc tccgaatata aatttacgca
      721 taatccgatt gaaaaagtta ttttaattcg tccgtgttcg agttaacggc ggcaggcgga
      781 tacgcacaaa cggtttccag cgccttaaat ttaaacggag aatcatttac ttaaaaaaat
      841 tgcggtcggc aaatccgcgt aaaatcgaaa attactagac tcgcggcttt aagatgagtt
      901 attttattag ttaggcgcta gcgcctttcc cactgccccg caaagcaaat tatgtcagcg
      961 acccaacgtc cgaaacgcga tttacgaccc gctctggttt ttttgagcgg cgactttctc
     1021 gccgtcccga ttccgctcga acgcgaggaa gtcatcttag gtcgcgcgct ggaagcggac
     1081 gtccgcgtca acgacaaaaa agtttcgcgc cagcacgcgc gcataacgac ggtgcaaaac
     1141 ggcgagacgg aagagccgag ctatatttta accgatttgg attcgcgcaa cggcatcttt
     1201 ctgaacggtc agcgcatcaa gcaggaaacc ttgcagaacg gcgacaaaat cactatcggc
     1261 gaacacatcc tgcgctttga gatgctggac gaaatcgacc gcgaatatca gcgccagatt
     1321 cgccgcctgc tctcgcacga cgatttgacc gggctgcttt caagccgctc gtttttttcc
     1381 gagctgcgcc gcgaagcctc gcagccggaa gcggaaaaac gtcagttctg cgttttgatg
     1441 atggacgtcg attttttcaa aaacgtcaac gacacttacg ggcatctgac cggcagcaaa
     1501 acgctggaag aaatcggttt gtgcatcaca cagattttgc gcagcggcga cgtcgcctcg
     1561 cgcttcggcg gcgaagaatt tgccgcgttt ttaatggacg ccgagctgac gcaaggcata
     1621 gtggcggcgg aaagaatccg ctcgaccatc gaagaacagg aattcagcgt catccggcag
     1681 ggcaagccga ccgaatcgca ccacatcacc atcagcatcg gcgtcgcttc ttttccggac
     1741 gattcgagcg acccgattga actggtcgaa atggctgatt cggcgcttta tcgcgccaaa
     1801 cgcgaaggca gaaaccgcgt ctgcgcctac cgcgaattga gcgaggaaga aacgaaaaaa
     1861 gcacagctcg cgcggcgcga agaacacgag cccgtctccg gcaaccccga ccttacgacg
     1921 cgaaaccaga tttccgagct ttaaataaaa agggaaacgt ctgtcgatag cggagcgcaa
     1981 aatgaattcc ggcttacatc tttgcgctcc gcattccggc gttttgcatt agttaagcct
     2041 ttagatataa aattttgcaa ctatttcgcc tcgctcgcgt gaaaacaatt cggtaagaaa
     2101 atcaaacgca aagacaaaga aaatttttcg tgataaactg gcgttttgtc gtttttcaga
     2161 cgattttgat gattggcaac aggtaatttg cggttgtcgg cagattattg caaacaacca
     2221 attgcctgct tccggctacc aatattaatt accgatttct taaaagcccg ataccaaaaa
     2281 agtgataaat aaactcaatc tcgcaacaca tccttttcgc aaccggacgt tgccgtatct
     2341 gatagcgctg gcgtgctgta cgctggcgat ggtcggcgta atcttcggtt ttgcgaagct
     2401 gcggcaaatc aaagcggaaa gtgaaatcgt ggagagcgaa gtccggctga tggaaacgca
     2461 gctgtcggaa ttaaagggca agggcgacca aatccagcag accttgacgc ccgaacagcg
     2521 cactctgctg attgcggcgc ataagctcgt cgctcagaaa agcttcggct ggtcgcgcct
     2581 gttcgccgat ttggaagacg ttctgccggg cggcgtcagc gtctcgcgca tcaatgtcga
     2641 aaacgtttat caaacgggcg accgcacgca agccgaattg caattgtccg tcttgagccg
     2701 cgattaccag ggcgtgatga atatgattga cacgatgaac tcgcgcccga ttttccaggc
     2761 tgagctgcgc gggcaggatt tgcagaaaaa agagtatttt acttacaccg aatacacgct
     2821 gcggctcatt tacactcagc cgctcggata ttcgaccgcg ccggcgccgg cgaacgacgt
     2881 cgcctccgtt aacgaaaaag gaggtcagca gtagcagaaa tgagcgaaaa tcttgacccg
     2941 aaagaccagc cgcagcaaca ggcggaaata attcgcgacg attttacgcc gcaggattcg
     3001 accgtcgtgg tggatgaaaa agaccgcacc gttattctca ccgaagacga aacgatagtc
     3061 atcgaaaaac agccgacctt tgacatcgcg ccgaaaaatc gcccgcggaa agtaaatctc
     3121 ggaattttcg gcacgaccga gtacatcgtc gtcgggctcg gtttgctgtc gatcgtcgcg
     3181 actctgctgt tctatatgtt tgtcgtcacg ccggagcagg cgaagctaaa caaccgcaaa
     3241 gccgagcgcg accaaaagga aaaagaattg tcgacgtcga aggacaaatt tgcagacttt
     3301 acgacgaccg aagaacacgt cgccaggctg gtcgggagcg tcgacgattt tgagagccgt
     3361 tttctgcccg tctccggcaa cggcagaacc gctttatacc agcgtctaaa taccttgatt
     3421 gccggttacg gtttgattaa cacaaccgga cccgattacg cgccgctcga aatcaatcag
     3481 cgcgaaggtc aaccggaagg cgaaaaaggc agagccaaat atcacagcct ttttccgggc
     3541 gtctatgtga cgatgaccgt cgaaggctcg tatcaaaatc tgcggcgttt tctgcgcgac
     3601 ctcgaaacca gccagcagtt tattattgtc agcacggtcg agcttgaacc ggcggaaaat
     3661 caagaccggc aaacggacgc gaccaccgtc acacaaacca cacagccgag cgtgaccagt
     3721 ccgaatcagc gatttgcgcg accgaacatc gacccgaccg gcgccgctaa tccgatgatg
     3781 agaaataatc cgggacaagt cacgaccacc acgacgacga ctcagcagac gacccgcccc
     3841 gcgccgcgcg gcagaacaat cggcgcaacc gtcagcctgc acatcgaact ggcggcgtat
     3901 tttagacgaa attcggcggt cagcatcctg ccgccgcagc agcagtaacc aacaagcgtt
     3961 tatgaacatt tttgaaggaa aaacaccatc ggaaaaaaag aaactcatcg cggcaatggt
     4021 tttgggcgga ctggctgtgc tgacgcttgt ttataatttc agcgggttga cgggcggcag
     4081 aaaaacgacc gtcacggtca gcgtctcgcc gacgccgtcg gcgaccatct cctcgtcgaa
     4141 cgggacgcag acaagcaaca ttaagctttt atctcaagac caaatcaacc aggaatggct
     4201 gaccagcccc gtcgtttaca cgcccggttc gttttacgcg ccggatgcgg gcagaaacat
     4261 tttcgccttt tacgagccgc ctcagccgac gccttatgag cccgttcccg tggttgaaac
     4321 gcctctgccg acgcctccgc cgacgccggt gccgacgccg ctgccgattt tggtcagcta
     4381 tgtttcgccg cagagcgttt acgccggttc gagaagtttc cggctggagg tcaacggcga
     4441 taaattcacg cccgattcgt tgattttctg gaacgggagc cagattccca ccagttttgt
     4501 caacacgcaa aagctgacgg cggacatctc cgccaatctg attgcgggcg aaggtcagag
     4561 gtcagtcgaa gttcgcacgc cggacggcaa actttattca aatccggtga tgatgaccgt
     4621 gcaggcgccg ccgcgaccga cagtccaata catcggcgcg gaaatagcga ggcgcggcaa
     4681 caataacacg gcttattttc gcgagccggg cgttccggtc gaattcggac ggcgcttaag
     4741 cgatattgtc aacgtaaaca ctgtgaacaa tcgctttcgg gtcgtcagca tttcggtcaa
     4801 cgaagtgatt ctcgaagata cgagtctggg tttccgccac cggctgccgc ttgcgcgacc
     4861 gtccggcgcg ggaggaggca ccacgggcgg cggacagacc acgcccagcc gcggcggttt
     4921 cgaccgaaat acgacgcccg gcagatttcc gaacgaaggg acaaccgtcg tgccgtataa
     4981 tccgactatg ccgcagacac aggaaattcc gggaattccg accaatattc cgcgctacgt
     5041 tccgccgacg ccgcagcagc agaaaaagga tgaagaagac gatgaggacg gcgataatta
     5101 aaaaagccat cagccatcag cggtcagcgg tcagcaggcg gaaaagaact ccgcaatccg
     5161 caatcggcaa tcggcagtcg ggttactcgc tcgtcgcgtt gatggcgatg atgacgcttc
     5221 tggcgattac tatgatggcg gttgcgccga gcgtttatca acaggttcag cgcgaaaaag
     5281 agctcgaatc catccggcgc ggcgaagaag tcgccgaggc gatacgtcaa tacgtcaatc
     5341 attacaacgg cgcgaagttt cccagttcga tggacgattt gctcgaaggc attccttacg
     5401 gaacaaagaa acgccagatt ttgcgcgaat cggcggcgat tgacccttta agcgaggacg
     5461 gcaaatggcg tctggtcggc gtcgaaagca agacgatggt aaatttcgcc cgccgcgtac
     5521 agaattacaa caacggcgct ttgccgacgt ccgaaaacgt ccatcagagc gttattaacc
     5581 ggctcggcag gattatcacc aatacggacg ccaacagcga agacgacatc aaggaatcag
     5641 ccgacgaaac cgacgaggac gacggcgaag tccgcaccga caatacgccg tttatcggcg
     5701 tggtcagcca gagcaaaaat aaatccttta tcgcttatta cgggattgag aaccattcga
     5761 aatgggtctt cacgccgctg tttcgcggaa ccggctcgac gccggtcggc ggctccacgt
     5821 ccagcaagcc aagaccggtt ctgcaacagt aaacgagcgt cataaaattt cctgattttc
     5881 catcgagctt aaaatttctt ttaaatccgc ttcattgagg ctgtaagtct cgttgcagaa
     5941 gccgcacgtc atcgtcgcgc ctttatcttc cgccagcatc gacgcgactt ccgcctttcc
     6001 cagagaacca atcagcgaaa cggctctttc aaacgagcag ttacaccgaa actcgatttc
     6061 cttttcctcc agcacttcaa aatcgatttc gccgagcgcc agccgcgcca ggtcctgcgg
     6121 cgtcgtctcg tcgttaatta cggtggtcag atgcggcgcg tgattaatcg tgtcttcaat
     6181 catcgtgatg atgtgctcgt ttgctccggg catcatctgc aatatcacgc cgcccgacgc
     6241 cttgacgaaa ggctcggtgt tctggagcag gacgccgagc attacggcgg acggaatttg
     6301 ctctgacttg gcgagataaa aggcgaaatc ttcggcgatc tcgccggaaa caatcggaac
     6361 ggagccgtga taaggctcgc gataaagccc gatgtcgaag cccgattcgc ggataacgtg
     6421 aaacattccg ccgccgacaa ttccgccgac gtcgaatttt ccgtccggtt taaacggcag
     6481 ctcagcctgc gggttgcgca cgtagccgcg cactttgcct tcggtattgg cttcggcgac
     6541 gatgccttcg attgcgcctt gagcgtctat tttcaccgtc aggcggtcaa attcctttaa
     6601 gctcgaaccc aaaaggagcg tgcccgtcaa aacgcgcccg aaagcggctg aggcggtcgg
     6661 cgaagtttga tgccggcgga cggcttcggc gacgatgtcg ctggtgatag ccgccatcac
     6721 gcgaattgtc ccgcccgcgg ctgttccgtg aatgagtttg tccataggta tagtttccga
     6781 aaatgagccc gggaaagtca aatttcaaat ttttcaaaaa ttatttaaga tttagaaata
     6841 atctttagcc cgcaaaagct aagaaattac attaagttac acgctggatt gagacggcgg
     6901 aaaaatttag cacaagatat tgtgtttttt ctattgacaa acgaaaatag cttttgtaag
     6961 attctcttca cttgagaagc gaaattcgac aactcaagtc tttttaagaa agttcggaaa
     7021 gattcttcca gtcccgcatt ccaagtaatg caagaacaat tataaaagag taaaattaaa
     7081 gattgagtcg tctttccaca cggcatttgg agaaggaaaa atccatgtcc gaaaacaaaa
     7141 atctaaacca ctcaattcta gaaattgagc agggtggaac tacgcagttt actaagttat
     7201 taaaaatatg tgaaaacttt tttggtgaat atcgaattaa tagtagtcac tatatattta
     7261 aaactccttg gcaaggtgac ccgcgaatca acttgcaaaa aggaaaag
//
LOCUS       DASWNE010000800         2843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_558186, whole genome shotgun sequence.
ACCESSION   DASWNE010000800 DASWNE010000000
VERSION     DASWNE010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558186"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VGB68_15620"
     CDS             complement(<1..389)
                     /locus_tag="VGB68_15620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050722.1"
                     /translation="MKSKIIFAVLFLVFATGYSFGQTAGDFEKKYGSAKYYEIRPYIL
                     VSPTFDKNGQLCRAEIIPSRHTLIKDKNAGTDFVEVPIRFSVTIYASSDTERLFPVTI
                     LDSAILREVFDELVPIKTRKGKAISSTV"
     gene            complement(401..2002)
                     /locus_tag="VGB68_15625"
     CDS             complement(401..2002)
                     /locus_tag="VGB68_15625"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HEY0050723.1"
                     /translation="MALQLRRKTHESIVYIDRDSAPRIMPFGEDFILEDIPIGTRVIY
                     PNPPIKGLPNREAAIRYAINHPLEAAPLYAQLEPGMRVTIAMDDISLPLPPMQTPDLR
                     QTMLEVVLDMCAANGVDDVHLIVANSLHRKMTAWEMERMVGKQIFNEFYPERFYNHDA
                     EDDENLITLGHTRHDEPLRVNRRAIESDILIYININLVPMDGGHKSVAVGLCDYESLR
                     AHHEPQTILDSHSYMDPPASELNHKVIRLGKLVDEHCNVFHIETALNNRMFPEGYDIL
                     TRNEDEFSFMDRMKWETMKRTFSKLPRRARRKMLHAIPAQYELIACYAGKTEPVHEKI
                     LEKNYQQYEIPVKGQCDIMITGIPDISPYNVYSALNPLLVQVMALGYHFNMFRNQPLL
                     RKGGVMIITHPCFDEFDHNFHPSYIEFFHRLLPESRDAFYLRDKYEREFATNPAYVEM
                     YRRGNAYHGAHPFFMWYWGENGRQHVGKVIVAGAENAHVPEILGWERANNLTEAIAQA
                     RSYMGRNAEITMLHQPVIGLCNVTD"
     gene            complement(2021..2386)
                     /locus_tag="VGB68_15630"
     CDS             complement(2021..2386)
                     /locus_tag="VGB68_15630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050724.1"
                     /translation="MTEAFQKLKSVDEKLEFQAANDWEKDMNHGYEDWNLPEKYKQRQ
                     FYLVRKIASDSQRAHEMTIWENNDGKMKLAAYQVVGSENGKTVFYGNQNTCLTTITPP
                     SIEIDLKQVIKNICTKIGC"
     gene            complement(2616..2774)
                     /locus_tag="VGB68_15635"
     CDS             complement(2616..2774)
                     /locus_tag="VGB68_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050725.1"
                     /translation="MALQLRRKTHESFSYSDSISDLPMLSIVGHAVAVNPDFRLKQTA
                     LCSTIGRF"
BASE COUNT          691 a          694 c          660 g          798 t
ORIGIN      
        1 acagttgaag aaattgcttt tcccttccgc gttttaatcg gaaccagctc atcaaaaact
       61 tctcgcagta ttgccgaatc caatattgta accggaaata gacgttccgt gtcggaactt
      121 gcgtagattg taacgctaaa ccttatcggc acttcgacaa agtcagttcc ggcgttttta
      181 tcctttatca gagtatgcct ggacgggata atttcagccc ggcaaagctg accgttttta
      241 tcaaacgtcg gactgaccag aatatacgga cgaatttcgt aatattttgc cgaaccgtat
      301 tttttttcaa aatcacctgc cgtttgcccg aaggaataac cggtggcaaa aaccagaaac
      361 aaaacggcga aaatgatttt tgatttcatc gattatttcc tcaatccgtt acgttacaaa
      421 gcccgattac cggctggtgc agcatcgtaa tctcggcgtt tctgcccata taggagcgcg
      481 cctgggcgat ggcttcggtc agattgttgg cgcgttccca gccgaggatt tcggggacgt
      541 gggcgttttc cgcgccggcg acgatgactt tgccgacgtg ctggcgaccg ttttcgcccc
      601 agtaccacat aaaaaacggg tgcgcgccgt gataggcgtt gccgcgtcga tacatttcca
      661 catatgccgg attggtcgca aactcgcgct cgtatttgtc gcgcagatag aatgcgtcgc
      721 gcgattcggg caaaagtcga tgaaaaaatt cgatatacga cgggtgaaaa ttatggtcga
      781 actcgtcgaa acacgggtgc gtgataatca tcacgccgcc ttttctcaaa agcggctggt
      841 tgcgaaacat attgaagtga tagccgagcg ccatcacctg caccagcaaa ggattcagcg
      901 ccgaataaac gttatacggc gagatgtcgg gaattccggt aatcattatg tcgcactgac
      961 ctttgaccgg aatttcgtat tgctggtaat ttttttcgag aatcttttcg tgaaccggct
     1021 cggtttttcc ggcgtaacag gctatcagct cgtactgcgc cgggatggcg tgcagcattt
     1081 ttcggcgcgc cctgcgcggg agctttgaaa acgtgcgctt catcgtttcc catttcatcc
     1141 tgtccataaa cgaaaattcg tcttcgttgc gcgtcaggat gtcgtagccc tcgggaaaca
     1201 ttcgattatt taaagccgtc tcgatgtgaa agacgttgca atgctcgtcg accagcttgc
     1261 cgagccggat gaccttgtga ttcagttccg acgcgggcgg gtccatatac gaatgcgaat
     1321 cgagaatcgt ctgcggctcg tggtgcgctc ttaagctttc ataatcgcac agaccgacgg
     1381 cgaccgattt atgcccgccg tccatcggga cgagattgat atttatataa atcaagatat
     1441 cgctttcgat ggcgcggcga ttgacgcgca acggctcgtc gtgacgcgtg tgccccagag
     1501 taattaaatt ttcgtcgtcc tcggcgtcgt gattgtaaaa gcgctcggga taaaattcgt
     1561 taaagatttg cttgccgacc attcgctcca tttcccacgc cgtcattttg cggtgcaagg
     1621 aattggcgac gatcaggtgc acgtcgtcca ctccgtttgc cgcgcacata tccagaacga
     1681 cttcgagcat cgtctggcgc aaatccggcg tctgcatcgg cggcaaaggc aaggaaatat
     1741 cgtccatcgc aatcgtcacg cgcattcccg gctcgagctg cgcgtaaagc ggcgcggctt
     1801 cgagcggatg atttatcgcg tagcgaatcg ccgcctcgcg gttcggcaag ccttttatcg
     1861 gcgggttcgg ataaatgacg cgcgtgccga tgggaatatc ttctaaaata aaatcctcgc
     1921 cgaaaggcat aatgcgcggc gcgctgtcgc ggtcgatgta aacgattgat tcgtgtgttt
     1981 ttctgcgtaa ttgcaaagcc ataattctga attgtatttc ttaacaacct attttcgtac
     2041 agatgttctt aattacctgt ttcaaatcaa tttctatcga aggcggagta attgtagtta
     2101 agcaagtatt ttggtttccg taaaatacag ttttgccgtt ctcacttccc acgacctgat
     2161 aagcagctaa tttcattttt ccgtcattgt tttcccagat agtcatttcg tgcgctcttt
     2221 gcgaatctga agcgattttc cgcaccagat aaaattgccg ctgcttgtat ttttccggta
     2281 aattccagtc ttcataaccg tgattcatat ctttttccca atcgttcgcc gcctgaaatt
     2341 ccagcttttc atcaactgat ttaagttttt gaaacgcttc cgtcatatcg gcggtaaatt
     2401 cttccctgga ataacaattt tttaaagata ttgagccttc ttcgtaaata tcgccgaatt
     2461 gtcgattctt aattttctcg tgaatgtttt ccagctttcc gacggaaatc tctttttcgc
     2521 ttttgcccgc gcagctacag gaaacgacgg ctgtaataaa aaacaaagct gaaatacata
     2581 acccgtgtcg aaacatttct cacctcactt taaattcaaa atcgcccaat cgtgctgcaa
     2641 agcgcggtct gcttcaaacg aaaatccggg ttgacggcga ctgcgtgacc gacaatcgag
     2701 agcatcggca aatccgagat gctgtcggaa taagaaaaag attcgtgcgt ttttctgcgt
     2761 aattgcaaag ccataaattt tataattgct tatttaactc taattattaa aggaatcgac
     2821 tcagaatcta tatatttatt tcc
//
LOCUS       DASWNE010000801         5040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_696214, whole genome shotgun sequence.
ACCESSION   DASWNE010000801 DASWNE010000000
VERSION     DASWNE010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5040
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696214"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..1312
                     /gene="dnaJ"
                     /locus_tag="VGB68_15640"
     CDS             203..1312
                     /gene="dnaJ"
                     /locus_tag="VGB68_15640"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEY0050726.1"
                     /translation="MSKRDYYEILGVSRTATDAEIKAAYRKMAIQHHPDKNPDNAEAE
                     EKFKEAAEAYSVLSDAQKRAAYDRFGHSGVGAGAGFGAGGAGFSGNIEDIFDLFGFGD
                     MFGGARGRRTSAQRGADLRYDLEITLEEAAAGKEEKLRIPRLEKCEECDGKGAEKGTQ
                     PEACITCQGSGQIRNQQGFFSVMRTCSNCRGTGQIIKNPCKKCHGAGRLEKEKSLEIK
                     IPAGVETGSRLRVSGEGEAGTNGGMHGDLFVVIHVREHELFERQGANLYASAPVSFAQ
                     AALGAEIQVRTLDGEETLKVPAGTQTGTVFRLKGHGMPNLGGRGKGDLFVAVTLVTPK
                     TLTKEQRKLLEKLAEVEDTEFNDESFMDKVRNIFG"
     gene            complement(1401..2345)
                     /locus_tag="VGB68_15645"
     CDS             complement(1401..2345)
                     /locus_tag="VGB68_15645"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEY0050727.1"
                     /translation="MHKKNKFLFPAFFGLLLCAAVLSFALSKLTAYGQNSNQMPVQNI
                     TQIAKLERPLTTAGKTVSDAPAISPTAAAENIRLKDSMAWAFGGKAQRGWYLYLSLIQ
                     ETIETDARAETIEFARAVAAWQNASGIDSTGVINEDTLYQMIKGWQARRLNSSQTPAS
                     ERLFDAPIADFYDPTHGADLLKVERETYDAYKRMVAAAAKELKLKTASSGELAPGESF
                     LKIISSYRSREHQARLRAASPNSGRAGLAVNSPHFTGNALDIYVGGEPVSTADANRAI
                     QVSTPAYKWLVKNASRFGFYPYYYEPWHWEYVPRNITK"
     gene            complement(2427..2879)
                     /locus_tag="VGB68_15650"
     CDS             complement(2427..2879)
                     /locus_tag="VGB68_15650"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEY0050728.1"
                     /translation="MSLKDRITGDLTAAMKAKDAARLSVLRMVKSNLMNRQIEKGGEL
                     TDEEITRALQSLVKQRKDSIEQYTNAGRNELAEKEQAEVVVIEEYLPQSATVEEIEAA
                     VAASISETGASSIKEMGAVMKAALARLQDKTADGKLVSETVKSKLQNL"
     gene            3072..4868
                     /locus_tag="VGB68_15655"
     CDS             3072..4868
                     /locus_tag="VGB68_15655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050729.1"
                     /translation="MKLRWRFGILAGIVLLVCGMYPQMKMWYLRGGDWQGHYAYNDID
                     EVAYAAYLRALIDGRARRNDPYTGRDDTAQHKQEESLFSIQFAAPYAVAIPARAFGIS
                     APTAMWLAGGIAAFLTALACFWLLGRITNDSFFAMTGTLVTLCGGALAAGEGAIGEIL
                     GTGFSYPYFPGFRRYIPAVPFAAFFAMCALVWLLVTGEDMRKRIVYCVLASLCFAFMV
                     FSYFYTWTTAAAWLACVVLVWLVVRPEGWQKDFKAFAALGIGCAIALVPYAILLSNRS
                     HTMDDVQLLVLTRAPDLLRVPAIICYAVLAMLILAILLKAINLKERSTLFAFSFALVP
                     LVVLNQQIITGRSLQPIHYQVFIGNYVAALALVVAIGLLWRGISPKNPDFSKTTLTIL
                     ALFAALWGIVECHYTVRVLDESNVDRDEGMAVARRLEELHRNETTRGVIMPFSMLQGD
                     DLPTVAPQSVLWARHQHVFAGLTWQENKERYYQYLYYMNLDENNLAQSMKKGDFVSII
                     ALFGWGRHTSRLNSGYKPLTYGEINEEAARYGEYRRNFGIAQASNPTLSYVVIPTDWQ
                     MNFTNLDKWYERGEAEIFGYYTLYQVKLRSGQ"
BASE COUNT         1152 a         1282 c         1360 g         1246 t
ORIGIN      
        1 ccagacttct tcgcaactca cttgaagaaa atcgcatctt atcgcaaaat taaaaaacgc
       61 cggttcattc gtttgggctg gcgttatctt tattttgtcc ttcgtcatta gtcgttcgtc
      121 cttagccggt cgaataaaaa ttaaggacgc aaattaattc gaggcatcga aggacgaagg
      181 acaaaggact aatgaccaat ctatgagtaa aagagattat tacgaaattt taggcgtttc
      241 gcgcaccgcg accgacgcgg aaatcaaagc cgcataccgc aagatggcga ttcagcatca
      301 tcccgacaaa aatcctgaca atgccgaagc ggaagaaaag ttcaaggaag cggcggaagc
      361 ttattcggtt ctttccgacg cgcagaaacg cgcggcttac gaccgtttcg gtcactcggg
      421 cgtcggcgcg ggcgctggtt tcggagcagg cggcgcggga ttttcgggca atatcgagga
      481 cattttcgat ttattcggtt tcggcgatat gttcggcggc gctcgcggtc gacgcacaag
      541 cgcgcaacgc ggcgcggatt tgcgatacga tttggaaatc actctggaag aagccgccgc
      601 cggaaaagag gaaaagcttc gtattccgcg tctggaaaaa tgcgaggaat gcgacggcaa
      661 aggcgcggag aagggaactc agcccgaagc ctgcatcacc tgccagggaa gcgggcagat
      721 tcgcaatcag caaggtttct tttcggttat gagaacttgc tcgaactgtc gcgggacggg
      781 acaaatcatc aaaaatccgt gtaagaaatg tcacggcgcc ggacgtctcg aaaaggaaaa
      841 atcgctggaa atcaaaattc cagccggcgt cgaaaccggc tcgcggctgc gcgtttcggg
      901 cgaaggcgaa gccggaacga acggcggaat gcacggcgat ttgttcgtcg tgattcacgt
      961 cagggaacac gaattatttg agcggcaggg cgcgaatctt tacgcttccg cgccggtctc
     1021 cttcgcgcaa gccgcgctcg gagccgaaat tcaggtgaga actctggacg gcgaagaaac
     1081 tctgaaagtt cccgccggaa cgcagaccgg aacggtcttt cggctgaaag gtcacggaat
     1141 gccgaatctc ggcggtcgcg gcaaaggcga tttgttcgtc gccgtgacgc tcgtgacgcc
     1201 gaagactctg accaaagaac agcgaaaatt gctggaaaaa ctcgccgaag tcgaagacac
     1261 cgaattcaac gacgaatcgt ttatggacaa ggtgcggaat attttcggat aaaagcgaca
     1321 ttcaagggat aaataaaaaa ggataaagct gattaatgtt cagctttatc cttttttatt
     1381 tatccctttt ttgaagtttt ttacttcgtt atatttcggg gcacgtattc ccagtgccac
     1441 ggctcatagt aatacgggta aaacccgaag cgcgaggcgt ttttgacgag ccatttgtaa
     1501 gcgggcgtcg acacctgaat cgcgcgattg gcgtcggcgg tcgaaaccgg ttcgccgccg
     1561 acataaatat ccaatgcgtt acccgtaaaa tgcggactat tgacagccaa accggcgcga
     1621 cccgaattcg gcgaggcggc acgcagtctt gcctgatgct cgcgcgaacg atacgacgaa
     1681 ataatcttca aaaaactttc tccgggcgca agctctccgc tcgacgcggt tttcagctta
     1741 agctcttttg ccgccgcggc gaccatacgt ttgtaagcgt cgtatgtttc gcgctcgact
     1801 tttaacaaat cggcgccgtg cgtcgggtcg taaaagtcgg caatcggcgc gtcgaaaagc
     1861 ctttcggacg caggcgtctg gctgctgttc agacgccgcg cctgccagcc tttaatcatc
     1921 tgataaagcg tgtcttcgtt tatcacgccg gtcgaatcga ttcccgaagc gttttgccag
     1981 gcggcgacgg cacgggcaaa ttcaatcgtc tcggcgcggg catcggtctc gattgtttcc
     2041 tgaatcaacg agaggtaaag ataccagccg cgctgcgcct ttccgccgaa agcccacgcc
     2101 atagaatcct ttagcctgat gttttcggct gcggcggtcg gtgaaatagc aggggcgtct
     2161 gaaaccgttt ttcccgcagt cgtaagcggg cgttcaagtt tggcaatttg agtgatattt
     2221 tgaacgggca tctgattgct gttttgcccg taagccgtaa gtttggataa ggcgaaactc
     2281 aaaactgcgg cgcacagcag caaaccgaaa aatgcgggaa acaaaaactt gttttttttg
     2341 tgcataatta aaagcgggtt ttaactatga tgtccggttt gccgcttgtg ttcgccgcca
     2401 attccggcat ttgaaattaa ccggaattaa agattttgca attttgattt gaccgtttcg
     2461 ctgacgagtt tgccgtccgc ggttttgtcc tgcagccggg cgagcgccgc tttcatcacc
     2521 gcgcccattt cctttatcga agacgcgccc gtttcggaaa tcgacgccgc gaccgccgct
     2581 tcgatttctt cgacggtcgc cgattgcggc aggtattctt caatcacgac gacttccgcc
     2641 tgttcctttt cggctaactc attgcgcccg gcattagtgt attgctcgat ggaatctttt
     2701 ctctgtttga ccagactttg cagcgctctg gtaatctctt catcggttaa ttcgccgcct
     2761 ttttcaatct gacggttcat taaattcgat ttcaccattc gcagaaccga aagccgggcg
     2821 gcgtctttcg ccttcatcgc ggcggtcaaa tcgccggtaa tcctgtcttt taaactcatt
     2881 atttataatc tcctgcgatt aattttagcg agtcgggagt ctattgtcca aagccggacg
     2941 agccgcaaat catatgaatt ccgcgaataa aagaaataaa aataatttgc ggaattcgcg
     3001 caatttgcgg ctcatccggc ttttcttatc cgcgtttatc tgtgctaatc tgtggtttct
     3061 ttttttccta tatgaaatta cgttggcgat ttggaattct ggcaggcatc gttctgctcg
     3121 tctgcggaat gtatccgcag atgaaaatgt ggtatctgcg cggcggcgac tggcaggggc
     3181 attatgccta caacgatatt gacgaagtgg cttacgccgc ctacctgcgc gctttgattg
     3241 acggcagagc gcgccgcaac gacccttata cgggacgcga cgatacggcg cagcacaaac
     3301 aggaagaatc gcttttctcg atacaattcg ccgcgccgta cgccgttgcg attccggcgc
     3361 gcgcctttgg aatttccgcg ccgaccgcga tgtggctggc gggcggaatc gccgcttttc
     3421 tgacggcgct ggcttgtttc tggctgctcg ggcgcatcac aaacgattcg ttttttgcga
     3481 tgacgggaac gctcgtcacc ctttgcggcg gcgcgctggc ggcgggcgaa ggcgccatcg
     3541 gcgaaattct ggggacaggt ttttcgtacc cgtattttcc cggcttccgg cgatacattc
     3601 ccgccgttcc gtttgcggcg ttctttgcga tgtgcgcgct tgtctggctg ctggtgacgg
     3661 gcgaagatat gcgcaaacgc atcgtttact gcgttctggc ttcgctgtgt ttcgccttta
     3721 tggtcttttc gtatttttat acgtggacga cggcggcggc gtggctggcg tgcgtcgttc
     3781 tcgtctggct cgtcgtgcgt cccgaaggct ggcagaaaga ttttaaagcg tttgcggcgc
     3841 tcggcatcgg ctgcgcgatt gcgctcgtgc cgtatgcgat actgctgtcg aatcgttcgc
     3901 atacgatgga cgacgtgcag ctgctggttt tgacgcgcgc gccggatttg cttcgcgttc
     3961 cggcgattat ctgctacgcg gtgctggcga tgctgattct ggcgattttg ctgaaagcca
     4021 tcaatctgaa ggaacgctcg acgctgtttg cgttttcttt cgccctggtt ccgctggtcg
     4081 ttctcaatca gcaaatcatc accggacgct cgctccagcc gattcactat caggttttta
     4141 tcggaaatta cgtcgccgcg ctggcgctgg tcgtcgccat aggcttgctc tggcgcggaa
     4201 tcagcccaaa aaatccggat ttttcaaaga cgactttgac gattctggct ttgttcgccg
     4261 cactctgggg aattgtcgaa tgtcattaca cggtgcgggt tctggatgaa tcaaacgttg
     4321 accgcgatga gggaatggcg gtggcgagac ggctcgaaga gcttcaccga aacgaaacga
     4381 cgcgcggcgt gataatgcct ttcagtatgc tgcagggcga cgatttgccg accgtcgcgc
     4441 cgcaatccgt cctttgggcg cgccatcagc acgttttcgc cggactcacc tggcaggaaa
     4501 acaaagagcg ctattaccag tatctttact atatgaatct ggacgaaaat aatctggcgc
     4561 agtcgatgaa gaaaggcgat ttcgtttcga taatcgcgct tttcggctgg ggcagacaca
     4621 ccagccgtct aaactccgga tacaagcctc tgacttacgg cgaaattaac gaagaagccg
     4681 cccggtacgg cgaatatcgc cgaaatttcg gcatcgcaca ggcttcaaat ccgacgcttt
     4741 cctacgtcgt cataccgacg gactggcaaa tgaattttac gaatctcgat aaatggtatg
     4801 aacgcggcga agccgaaatc ttcggctact acacgcttta tcaggtcaaa ttgagaagcg
     4861 gtcaatagtc gttttcttat agtcgtttgc tgcaaaagaa aggcgaaaat ataaatgaga
     4921 gctcgatttt tactcaatct tcaaaggaca gtaagacgaa gaaccgtcga ctaataaaaa
     4981 aagaccgccg aaaaccggcg gtcgcatttg gtattactta taaatataaa aacaactagg
//
LOCUS       DASWNE010000802         5941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_338521, whole genome shotgun sequence.
ACCESSION   DASWNE010000802 DASWNE010000000
VERSION     DASWNE010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338521"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VGB68_15660"
     CDS             <1..685
                     /locus_tag="VGB68_15660"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY0050730.1"
                     /translation="GPQTEAALRRFQSEHGLTRDGIYGPNTFRALQSATPRTNGNGNG
                     NTPQTEPPVNDGEAKEYRRWNVYSTGHRPARLSDGYEDLQAHHDYQSVNYVMRGLRLT
                     HRLEARDIVLTRPGQSNFGQAVPSPLEGKVLFAGNENDGYGNKVVVQNERTGQIMMVG
                     HLQSVNVRRGDTVSYGQNLGGQGSTGHSSGAHIHINADSSVIRRWVADLADGKFDGVR
                     GRFDVGQRP"
     gene            complement(767..1171)
                     /locus_tag="VGB68_15665"
     CDS             complement(767..1171)
                     /locus_tag="VGB68_15665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050731.1"
                     /translation="MSVKKTSSPLSSGSEALEPLSSKDELRRGAKAEKNFEAALAEVA
                     GQIEQASETGQTGAPARSALSQIASGSDLNTPEGAMQAVRESARFLVNSRLNEELRDS
                     SQGKKMTDDLSEYIAKDPFMHRKILSILHRLK"
     gene            1578..2537
                     /locus_tag="VGB68_15670"
     CDS             1578..2537
                     /locus_tag="VGB68_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050732.1"
                     /translation="MGIEINGVSASVRSDAIHSPERDNFQPQIDSKTENPPVETFFRQ
                     ANETLSDAYAARTGLSTEINAFSVRNRAVRQNPPPDTNFDGHFVGANGQTFSPDTALN
                     DVPAVTPRGGVTNNETLIYVNGIRTNVQGQLNSLQAIADQTGSRVVGVHNATQSSAAD
                     ILQSLGDKLDIGSNPAVDTLADTVYNELRAGRDVHLLAHSQGAIITSRALQDVKNRLM
                     AEDGMSRRDAERLMSNIKVETFGGASRRFPDGPRYVHYVNRNDGVPQAFGLRSWLNPF
                     AHAGRDAVTHYFREGKPLVSHGFEKFYLPERVPFEQARQGNFH"
     gene            2550..2966
                     /locus_tag="VGB68_15675"
     CDS             2550..2966
                     /locus_tag="VGB68_15675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone"
                     /protein_id="HEY0050733.1"
                     /translation="MTREEAKQQLETFFRRLGSEASIFNEQKFVQARIGESFVGFEFD
                     ETDDLLSCQALIYRFRREPTDEILDALFAEESDANSGGGRIVFDSETFSLYLQRDFEE
                     KIDDASFYEQVNALAWASMVWNGEIVEHAAAKVSAS"
     gene            3172..5187
                     /locus_tag="VGB68_15680"
     CDS             3172..5187
                     /locus_tag="VGB68_15680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050734.1"
                     /translation="MALKVDGGASSSVRREETDYKKTEVKSNPIRRDETTNGAVYANR
                     SDNNLQANVVRLKLNVQVGANVVAPTFTQAQARRRADEIIAANGGKDNLDTEGVGRDL
                     ANLARQNPADAWAVTQEILGAEIDQSGRGKIEENDKDEIAQSFADNLSDGELSAVGQN
                     SGGRAMLERLQAHLLSGSVHDDERDTALRLETAAKGYPPPELTGNPERDIQTVAEDLR
                     NIPADKRDDYLNEVLAHPFGRQALQRAGILNNADRDVLAGAIDEAFRQNPTAIRDHLN
                     AVTDLEKNYPYYEWTGLADVVRRTGNDDLIAGYAKHAMSVAATGGAEKGAWSVDALTA
                     LGGMSPEGLRNFINFGSARPPQYGSPSTIGRFIEQNRAAFDAMLTQSAGFLGGPANNS
                     GFENGWAFHPAMGDLLNVASRMTDASGKLTSEALDIFKTVVPLTGDNFFTKEAAGKFF
                     IEHAQQITDTFALKRNPDGAANRNFDPNVLKDFFINVVYSPISDLLQYNGASMVEAIM
                     GDGGGNDGVIGQVAQNIMSRAAQPGADVTYLGQEIGYLYGAISGGFLDSVEAYRDKFD
                     DDKKFREFLYGAVNKGLGALGEKVGLPLEKVGEFVRKLIEAEAETDKTERLKKFQEAF
                     LGLKNEMSQSLLDFQQRNPRAEGLETNFTIAWTDFITSYLATDWIAD"
     gene            complement(5346..>5941)
                     /locus_tag="VGB68_15685"
     CDS             complement(5346..>5941)
                     /locus_tag="VGB68_15685"
                     /inference="COORDINATES: protein motif:HMM:NF012236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EscN/YscN/HrcN family type III secretion system
                     ATPase"
                     /protein_id="HEY0050735.1"
                     /translation="AIAEYFRDQGKKVLFLMDSVTRFARALREVGLAAGEPPARAGFP
                     PSVFSELPRLLERTGNSDKGSITAFYTVLVEGDDMTEPIADETRSILDGHIVLSRDLA
                     AQNHYPAIDINESVSRVMSGIAEMRHIEAAGKLREVLATYAAQKDLILIGAYKKGSDP
                     KTDYAISKIEAVNNFLRQKTHEQMPAEIAIQKLIQLF"
BASE COUNT         1384 a         1601 c         1675 g         1281 t
ORIGIN      
        1 cggaccgcaa acggaagccg cgctcaggcg ttttcaatcc gaacacggct tgaccagaga
       61 cggcatttac ggacccaaca catttcgcgc gctgcaaagc gcgacgccgc gaacgaacgg
      121 caacggtaac ggcaacacgc cgcaaaccga gccgcccgta aatgacggcg aagcgaaaga
      181 atatcgccgc tggaacgtct acagcaccgg gcatcgtccg gcgcggctct cggacggtta
      241 cgaagattta caggcgcatc acgattatca atcggtcaat tacgtgatgc gcggtttacg
      301 gttgacgcat cggctcgaag cgcgcgacat cgttttgacg cgaccgggac agagcaattt
      361 cgggcaagcc gtcccgtcgc cgctggaagg caaagtttta tttgcgggca acgaaaacga
      421 cggttacggt aataaagtgg tcgtgcaaaa cgagcgcacc gggcagatta tgatggtcgg
      481 acacttgcag tcggtcaacg tccgccgcgg cgacacggtt tcttacgggc agaatctcgg
      541 cgggcagggc tcgaccgggc attcgagcgg cgcgcacatt cacatcaacg ccgattcgtc
      601 ggtcatccgg cgctgggtcg cggatttagc cgacgggaaa tttgacggcg tgcgcgggcg
      661 cttcgacgtc ggtcagcgtc cgtgataaag gcagaacgca aaaaacaaag cgcaaaataa
      721 agaaagttct ttattttgcg ctttgctttt tgcgtttata gacgacttat ttcaggcggt
      781 gcagaatgct gaggattttg cgatgcataa acgggtcttt ggcgatatat tcgctcaaat
      841 cgtcggtcat ctttttgccc tggctggaat cgcgcagctc ttcgttcaga cgcgaattga
      901 ccagaaaccg cgctgattcg cggacggctt gcatcgcgcc ttcgggcgtg ttcaggtcgg
      961 agccggaggc gatctggctc agggcgctgc gcgccggagc gccggtctga ccggtttcgc
     1021 tagcctgttc gatttgcccg gcgacttccg ccagcgccgc ctcgaaattt ttctcggctt
     1081 tcgcgccgcg ccgcaattca tcttttgagc tgagcggctc taacgcttcg gagccgctgc
     1141 tgagcggcga acttgttttc ttgaccgaca ttgcttttaa cctcaaactt tttcgcttaa
     1201 cacaaagatt atatttgaat ccggcgggtt tgttaaacga aaatttccgg agaattcgcg
     1261 gtgagcacgg ataattccgt taaaattccc ggtcgccgat gttcccgccg aagctccgga
     1321 tgagccttgc ggcgaaacgg attcccgttt ctcacctgtt tatagtatcg acgctttccc
     1381 gaaaaggttt cccagacttt ttatccgctt gttgccgccg ccgtctttcg actcgataaa
     1441 ttttgctgaa tcgggcgcgc cggcagttca tttttcgctt atttgataac tttcgacggc
     1501 agctcgaaaa aaatcggaaa ctttggaagt tttctgccga taatttgtct gtcgaagtaa
     1561 attcacggag aaaagtaatg ggcatagaaa taaacggcgt ctctgcaagc gtccgctccg
     1621 acgcgattca ttcgcccgaa cgcgacaatt ttcaaccgca aatcgattcg aaaacggaga
     1681 atccgccggt cgaaacgttt ttccggcagg cgaatgaaac tttgagcgac gcttacgctg
     1741 cgcggacagg gctttcgacg gaaatcaacg cgttcagcgt cagaaatcgc gccgttcggc
     1801 agaatccgcc gcccgacaca aatttcgatg gtcatttcgt cggcgcaaat ggtcagactt
     1861 tttctcccga tacggcttta aacgacgttc ccgccgtcac gccgcgcggc ggcgtcacca
     1921 acaacgaaac tttgatttac gtcaacggaa tcaggactaa tgtgcagggt cagctgaaca
     1981 gtctgcaggc gattgccgac caaaccggct cgcgtgtcgt cggcgtccac aatgcgacgc
     2041 agagttccgc cgccgatatt ctgcaaagtc tgggcgacaa gctcgacatc ggcagcaatc
     2101 cggcggtcga tactctggcg gacacggttt acaatgagct gcgcgccggt cgcgacgtgc
     2161 atctgctcgc gcacagtcag ggcgcgatta tcacgagccg cgccttgcag gacgtgaaaa
     2221 atcgcctgat ggctgaagac ggaatgtcgc ggcgcgatgc cgagcgtctg atgagcaata
     2281 ttaaagtcga gaccttcggc ggcgcttcgc ggcgttttcc cgacggaccg cgatacgttc
     2341 actacgtcaa ccgcaacgac ggcgtgccgc aggctttcgg cttgcgctcg tggctgaacc
     2401 cgttcgctca cgccgggcgc gatgccgtga cgcattattt ccgcgagggc aagccgcttg
     2461 tctcgcacgg cttcgagaaa ttttatttgc cggaacgcgt cccgttcgaa caggcgcggc
     2521 agggaaattt tcattaagcg gatttctaaa tgaccagaga agaagcaaaa cagcagctgg
     2581 aaactttttt tcgcaggctc ggcagcgaag cctcgatttt taacgagcag aaattcgtcc
     2641 aggcgcgcat cggcgaatcg ttcgtcggtt tcgagtttga cgagacggac gaccttttgt
     2701 cgtgtcaggc tttgatttat cgctttcgcc gcgagccgac cgacgagatt ctcgacgcgc
     2761 ttttcgccga agaatccgac gccaacagcg gcggcgggag aatcgtcttt gacagcgaaa
     2821 ctttttcgct ctatctgcag cgcgatttcg aggaaaaaat cgacgacgcc tcgttttacg
     2881 agcaggtcaa cgcgctggcg tgggcgagca tggtctggaa cggcgaaatc gtcgagcacg
     2941 ccgccgcgaa agtttcggca agctgaatta aaacccgttc ggaaacgcgt ttaatgcaaa
     3001 acgcggaatg cgaagcgcga aatcaaacag gatttgaatt ttgcgttttg catttttatt
     3061 ttccgcagaa aacttctctc gtttccgaaa taatcgggaa actttgcgcc ggaaacgccg
     3121 ataacttaac tgagccctat catttcaaaa ctgacttcgg gagagcgcat tatggcattg
     3181 aaagtggacg gcggcgcgag ctcaagcgtt cgccgggaag aaacggatta caaaaaaacc
     3241 gaagtaaaat cgaatccgat tcgccgcgac gagacgacta acggcgcggt ttacgccaat
     3301 cgcagcgaca ataatctgca ggcgaatgtc gtgcgcttaa aattaaatgt tcaggtcggc
     3361 gccaatgtcg tcgctcccac tttcacgcag gcgcaggcac gccggcgagc cgacgaaatt
     3421 atcgccgcca acggcggaaa agataatctg gacacggaag gcgtcgggcg cgatttggca
     3481 aatctggcgc ggcagaatcc ggcggatgcc tgggcggtca cgcaggaaat tctcggcgcg
     3541 gaaatcgacc agagcggtcg cggcaaaatc gaggaaaacg acaaggacga aatcgcgcag
     3601 agttttgccg acaatctttc cgacggcgaa ttgagcgccg tcgggcagaa ttcgggcggc
     3661 agggcgatgc tcgaaagatt gcaggcgcat cttttgtcgg gcagcgttca cgacgacgag
     3721 cgcgacacgg ctctccgatt ggaaacggcg gcgaaaggtt atccgccgcc ggagctgacc
     3781 ggcaatccgg agcgcgatat tcaaaccgtc gccgaagatt tacgaaatat tccggcggac
     3841 aagcgcgacg attatttaaa cgaagtgctg gcgcatcctt tcggcaggca ggctttgcag
     3901 cgcgctggaa tcctgaacaa cgccgaccgc gatgttttgg caggcgcgat cgacgaagct
     3961 tttcgccaaa atccgaccgc gatacgcgac catctgaacg ccgtcaccga cctcgaaaaa
     4021 aattatccgt attatgaatg gaccggtctg gcggatgtcg tgcgccgcac gggcaacgac
     4081 gatttaatcg ccggttacgc caaacacgcg atgagcgttg cggcaacggg cggcgctgaa
     4141 aaaggcgcgt ggtcggtcga cgcgctgacg gcgctcggcg gaatgtcgcc cgaaggcttg
     4201 cgcaatttta ttaatttcgg ctcggcaaga ccgccgcaat acggcagccc ttcgaccatc
     4261 ggacgtttta tcgagcagaa cagagcggcg ttcgacgcta tgctgacgca gtctgccgga
     4321 tttctgggag gaccggcaaa caactccgga tttgaaaacg gctgggcatt ccatccggcg
     4381 atgggcgatt tgttgaacgt cgcttcgcgg atgaccgacg caagcggaaa attgacttcc
     4441 gaggcgctgg atattttcaa aaccgtcgtg ccgctgaccg gcgataattt ctttaccaag
     4501 gaagcggcgg gcaaattttt tatcgagcac gcgcagcaga ttaccgacac gtttgcctta
     4561 aagcgcaacc cggacggcgc tgcgaatcga aactttgacc cgaacgtttt gaaagatttc
     4621 tttatcaacg tcgtctattc gccgatttcc gatttgctgc aatacaacgg cgcgtcgatg
     4681 gtcgaagcga ttatgggcga cggcggcggc aacgacggcg ttatcgggca ggtcgcgcag
     4741 aacattatga gccgcgccgc gcagccgggc gctgacgtaa cttatctcgg acaggaaatc
     4801 ggctatcttt acggcgccat cagcggcggc tttctcgatt cggtcgaggc ttatagggac
     4861 aaattcgatg acgacaagaa attccgcgaa tttctctacg gcgcggtcaa caaaggtctg
     4921 ggcgcgctcg gcgaaaaagt cggtctgccg ctcgaaaagg tcggcgaatt cgtccggaaa
     4981 ttaatcgaag ccgaagccga aaccgacaag accgagcggc tgaaaaaatt tcaggaagcg
     5041 tttctcggat tgaaaaacga aatgagccag agcctgctcg acttccagca gcgaaatccg
     5101 cgcgccgaag gcttggaaac gaatttcacg atcgcgtgga cggattttat aaccagctat
     5161 ctggcgaccg attggattgc cgattaataa aaagtttatc aagcgggcaa gtaacttctc
     5221 cttcaacttc ggctgtgatt attggcaatc acagcctttt ttttggaaac gatgaatgcg
     5281 gaacgatgaa ttcgatgcgt agaatctgca attcatcgtt ccgcattccg cattcatcgt
     5341 tttatttaaa acaactgaat caatttttga atggcaattt cggcgggcat ctgttcgtgg
     5401 gttttttgtc gtagaaaatt gttgaccgct tcgattttgg aaatggcgta gtcggttttc
     5461 gggtcgctgc cttttttata cgctccgatt aaaatcaaat ccttctgcgc ggcgtaagtc
     5521 gccaggactt cgcgcagctt tccggcggct tcgatgtgcc gcatttccgc gatgccgctc
     5581 atcacgcgcg agaccgattc gttgatgtca atcgccggat aatgattctg cgccgccaaa
     5641 tcgcgcgaca gaacgatgtg accgtccaga atcgagcgcg tctcgtcggc aatcggctcg
     5701 gtcatatcgt cgccttcgac cagaaccgtg taaaacgccg taatcgagcc tttgtcggaa
     5761 tttcccgtac gttccagaag gcgcggcagc tccgagaaaa ccgacggcgg aaaaccggcg
     5821 cgcgccggag gctcgcccgc cgccagaccg acttcgcgca gggcgcgggc aaaacgcgtc
     5881 acggaatcca tcaaaaacaa aacctttttg ccctggtcgc ggaaatattc ggcaatcgcc
     5941 g
//
LOCUS       DASWNE010000803         2051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_696323, whole genome shotgun sequence.
ACCESSION   DASWNE010000803 DASWNE010000000
VERSION     DASWNE010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696323"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..340)
                     /gene="trxA"
                     /locus_tag="VGB68_15690"
     CDS             complement(14..340)
                     /gene="trxA"
                     /locus_tag="VGB68_15690"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEY0050736.1"
                     /translation="MGNFAKQATDTNFETDVLSSDKPVLVDFWAEWCGPCRMIAPSVE
                     AIAEDYKDKASVYKMNVDENMNVPQRYGIRGIPTLIVFKNGEEKERIVGAVSREAIAN
                     VIEKYV"
     gene            532..762
                     /locus_tag="VGB68_15695"
     CDS             532..762
                     /locus_tag="VGB68_15695"
                     /inference="COORDINATES: protein motif:HMM:NF022716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3072 domain-containing protein"
                     /protein_id="HEY0050737.1"
                     /translation="MTDKLTQQHADESNTIKNPEDWTTGDEPMTGAQRSYLKTLSEEA
                     KEDFDENLTKAEASVRIDELQQKTGRGASAGK"
     gene            complement(836..1279)
                     /locus_tag="VGB68_15700"
     CDS             complement(836..1279)
                     /locus_tag="VGB68_15700"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR03562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY0050738.1"
                     /translation="MAIRTAKATWEGGLTDGKGTMALGSGAFEGSFSFNTRMGDEPGT
                     NPEELIGAALAGCYTMALNATLEKEGSKPNSVRTEAKVHFGKDGEGFAITRIDLQTEA
                     DIDGIEDAKFQEIAQQVKKACPVSKALTGTEIALDAKLIKAQAAG"
     gene            complement(1392..>2051)
                     /locus_tag="VGB68_15705"
     CDS             complement(1392..>2051)
                     /locus_tag="VGB68_15705"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0050739.1"
                     /translation="KRLGIETIDLYYQHRVDPETPIEDTVQAMAELVKEGKVRYLGLS
                     EASAETLRRADSVHKITALQSEYSLWTRDVEDNDVLATCRDLGVGFVAYSPLGRGFLS
                     GEIKRFEDLAEDDYRRFSPRFQGENFDKNIKLVEKVEEIAKEKGITASQLALAWVLAQ
                     GNDVVPIPGTKRRKYLEQNAAAADVILSQEDLASIEDVFPKDAAAGLRYPEAMMASVN
                     R"
BASE COUNT          480 a          580 c          441 g          550 t
ORIGIN      
        1 ctaaaatcaa aaattaaacg tatttttcaa ttacgttggc aatcgcctcg cgcgagacgg
       61 cgccgacgat gcgctctttt tcctcgccgt ttttgaaaac gattaaggtc ggaatgccgc
      121 gaatgccgta gcgctgcggg acgttcatat tttcgtccac gttcatttta taaacgctcg
      181 ccttgtcttt gtaatcctcg gcgatggctt cgaccgacgg cgcaatcatc cggcaaggtc
      241 cgcaccattc cgcccagaaa tcgaccagca cgggtttgtc cgaagacaaa acatccgtct
      301 caaaattcgt atccgttgct tgttttgcaa aatttcccat atgtttctcc taaaaattat
      361 tcgtaaaatt acgcgaaaac ggttaaattc ctttttcgca accgtttatt tcatcttagc
      421 ctgccgattg ttcgtttgcc aaatcttttg tttttctccg gtggcacgat ttgtgcagta
      481 tgtaggctag tgtttaatca ctgaaacaaa atcgaacacc aggagtaatt tatgactgac
      541 aaacttacac aacaacacgc cgatgaaagc aacaccataa aaaatccgga agattggacg
      601 accggcgacg aaccgatgac cggcgcgcag cgctcttatc tgaaaaccct gagcgaagaa
      661 gccaaggaag actttgacga aaatctgacc aaagccgaag cttccgtgcg catcgacgag
      721 ctgcagcaaa aaaccggacg cggcgcttcc gccggaaaat aaagtccaat ccatcaatcc
      781 atcgggaaag gatatttata ttttatttat cctttcccga tacttctttc aaatatcaac
      841 cagccgcctg cgctttgatt aatttagcgt caagcgcgat ttccgttccc gtcaaagcct
      901 tgctgaccgg acacgctttt ttcacctgtt gagcgatttc ctgaaacttc gcgtcttcga
      961 tgccgtcgat gtcggcttcg gtctgcaaat caatgcgcgt gatggcaaag ccttcgccgt
     1021 ctttgccgaa atgaactttc gcctcggtgc ggacgctgtt cggcttgctg ccttcctttt
     1081 ccagagtcgc gttcagcgcc atcgtgtaac agcccgccag cgccgcgccg attaattctt
     1141 cgggattcgt tcccggctcg tcgcccattc gcgtattaaa cgaaaatgag ccttcaaatg
     1201 cgccgctgcc cagcgccatc gtgcctttcc cgtcagtcaa accgccttcc caagttgctt
     1261 tcgccgttct aatagccata tttttttacc cttcccttat tttattaatg atgaatgata
     1321 aacgcggaac gcggaattgc agatatttag tatcgaattc atcgttcatc gttccgcatt
     1381 catcgtttct cttacctgtt tacgctcgcc atcatcgctt ccgggtagcg caagcccgcc
     1441 gccgcatctt tcggaaaaac gtcttcgatg ctcgccaaat cttcctggct caaaataacg
     1501 tcagccgcag ccgcgttttg ttccagatat ttgcgtcgtt tcgttcccgg aatcggcacg
     1561 acgtcgttgc cctgcgccag aacccaggcg agcgcgagct gcgaagccgt aatgcctttt
     1621 tctttcgcga tttcctcgac tttttcgacc agcttaatat ttttgtcgaa attttcgccc
     1681 tgaaaacgcg gcgaaaaacg gcgataatcg tcttccgcca aatcttcaaa ccgcttgatt
     1741 tcgcccgata aaaatccgcg tccgagcggc gaatacgcga caaaaccgac gcccaaatcg
     1801 cggcaagtcg ctaaaacgtc gttgtcttcg acgtcgcgcg tccacaaaga atactcgctc
     1861 tgcaaagccg tgattttgtg aaccgagtcg gcgcggcgca gagtttccgc cgacgcttcc
     1921 gacaagccta agtaacgaac ttttccttct ttcaccagtt ccgccatcgc ttgaacggtg
     1981 tcttcaatcg gcgtttccgg gtcgacgcga tgctgataat aaaggtcgat cgtttcgatt
     2041 cccagacgct t
//
LOCUS       DASWNE010000804         1901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_558320, whole genome shotgun sequence.
ACCESSION   DASWNE010000804 DASWNE010000000
VERSION     DASWNE010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558320"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /gene="ribD"
                     /locus_tag="VGB68_15710"
     CDS             complement(<1..616)
                     /gene="ribD"
                     /locus_tag="VGB68_15710"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEY0050740.1"
                     /translation="MTQRALELASFGVGQVSPSPLVGCVIVANGGETIGEGTYLYENV
                     THAEVLALRQAGKRANGATAYVSLEPHAHTGRTKPCTEALIEAGIRRVVCPVEDPNPL
                     VSGKGFAKLKENGIEVVTGILRDEASRLNEKFCVWHQKARPFVHLKMAMSFDGRISLS
                     SSVSTALSGAESAARVQSLRHEHDAILVGGNTAAVDNPSLTDRSG"
     gene            complement(642..857)
                     /locus_tag="VGB68_15715"
     CDS             complement(642..857)
                     /locus_tag="VGB68_15715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050741.1"
                     /translation="MNPDEMFKLAEKVYEGLSKEDIAEMEKVMLDRSNFSGERTKRLE
                     ELLEGVNEDNLHGETDWGKPVGKEIIE"
     gene            complement(1034..1465)
                     /gene="tnpA"
                     /locus_tag="VGB68_15720"
     CDS             complement(1034..1465)
                     /gene="tnpA"
                     /locus_tag="VGB68_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_044282811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEY0050742.1"
                     /translation="MSELIRKEHNVSVLLYHLVCPTKYRKAVITPDVDEKLKEICEEI
                     SLRYEIEFLEIGADADHVHFLVQSVPTYSPTKIVRTIKSITAKEIFRQMPEVKKQLWG
                     GEFWSDGYYISTVGAHSSEEAMKQYVKNQGGVSESTQPSLF"
     gene            complement(1501..>1901)
                     /locus_tag="VGB68_15725"
     CDS             complement(1501..>1901)
                     /locus_tag="VGB68_15725"
                     /inference="COORDINATES: protein motif:HMM:NF025224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEY0050743.1"
                     /translation="THLEVLLASDNYLTTLPVEILQLTNLRHLHLAANRLNIFPKEMN
                     QLVNLREIDLSANNLRKVPKEIFRIGNLEDIGLGNNPLTELPVEISSLKNLKFLHLHD
                     TKLKLPPEIEDGCGSPYRIISYYFNRSLKV"
BASE COUNT          462 a          474 c          373 g          592 t
ORIGIN      
        1 tcccgcttct gtccgtcaaa ctcgggttat caaccgccgc cgtatttccg ccgactaaaa
       61 tcgcgtcgtg ttcgtgccgc aaactctgca cccgcgccgc cgattccgcg ccggaaagcg
      121 ccgtcgaaac gctgcttgag agcgaaatcc tgccgtcgaa cgacatcgcc attttcaaat
      181 gcacgaacgg gcgcgccttt tgatgccaga cgcagaattt ctcgttcaac cgcgacgctt
      241 cgtcgcgcaa aattcccgtc acgacttcga ttccgttttc ctttaacttg gcaaaacctt
      301 tcccggaaac cagcggattc gggtcttcca cggggcagac gacgcgccgg attcccgctt
      361 cgatcagcgc ttcggtgcac ggcttcgtcc tgcccgtgtg cgcgtgtggc tccagcgaaa
      421 catatgccgt cgcgccgttt gcccgtttcc cagcctgccg cagcgcgagc acttccgcgt
      481 gcgtcacatt ttcgtaaagg taagttcctt caccgattgt ctcgccgcca tttgcgacaa
      541 tcacgcagcc gacgagcggg ctcgggctga cttgcccgac gccgaatgac gcgagttcca
      601 aagcgcgctg cgtcattttt atatcaaatt ctgaaaaagc gttattcaat tatttctttt
      661 ccaacaggtt tgccccaatc agtttctccg tgaagattat cttcattaac tccttccaac
      721 aattcttcga gtcttttggt tcgttcacca gagaaatttg aacggtcaag cattactttt
      781 tccatctcgg caatatcttc tttggaaaga ccttcataaa ccttttcagc taacttaaac
      841 atttcgtcgg gattcatctt cttattaatc atgcgaaatt attttcttaa ttcgcttttt
      901 cgtccaaacc taaaactgat ttattttcgc cttttcccaa aatctttttg cccgcgtttt
      961 tcaaaactgt tgccggattt aaatgcaatc caaaatgttt taataaattt ccgcgcggca
     1021 gagccgcgag gtattaaaag agcgatggct gagttgactc acttactccg ccttgatttt
     1081 tgacatattg tttcatcgcc tcttctgaac tatgcgcgcc gactgtgctg atgtaatatc
     1141 cgtcagacca aaactcgccg ccccataatt gcttttttac ttccggcatc tgccgaaaga
     1201 tttccttggc ggtgatgctt ttaattgttc gcactatttt cgtcgggctg taagtcggaa
     1261 cactctgcac taaaaaatgc acatggtccg cgtcagcacc gatttccaga aactcaattt
     1321 catagcgcaa cgatatttct tcgcatattt ctttgagttt ttcatcaaca tcaggcgtta
     1381 ttacagcttt ccgatatttt gtcggacaga ccaaatggta aagtaaaacg ctcacgttat
     1441 gttccttacg aatgagttcg ctcattcaca cagtatattc cgtagcagag ctacggggaa
     1501 ttagaccttt aaggaacgat taaaataata tgagataatt cgatatggac ttccgcaacc
     1561 atcttcaatt tccggtggaa gttttagctt agtatcatgt agatgaagga acttcagatt
     1621 tttcaaacta ctaatttcaa ctggcaattc agttaatgga ttattaccta aaccaatgtc
     1681 ttctaaattc cctattctaa atatttcttt tgggactttt cttagattat ttgcagataa
     1741 atctatttct cttaaattaa ctaattgatt catttctttt ggaaatatat ttaatctatt
     1801 agcggcgagg tggaggtgtc gtaagttagt caattgaagt atttctaccg gtaaagtagt
     1861 tagataattg tctgatgcta ataagacttc taagtgtgtt g
//
LOCUS       DASWNE010000805         1675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_558406, whole genome shotgun sequence.
ACCESSION   DASWNE010000805 DASWNE010000000
VERSION     DASWNE010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558406"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1111)
                     /locus_tag="VGB68_15730"
     CDS             complement(<1..1111)
                     /locus_tag="VGB68_15730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050744.1"
                     /translation="MKKIILNILVLLAAFVSSFAQTSQTTLFRQPTVSRTDIVFSYGG
                     DLWIVTRAGGDARRLTTGIGIETNPYFSPDGNWIAFTGEYDGNADVYIVPATGGVPKR
                     LTFHPSVDEVSGWTADGRSVLFASTRNSGFGASRLYTFPVDGSGLPFEIPLPMADRGW
                     LSPDGQYVAYEPLSQWQSDWKRYKGGQTQPIWIARLSDSTIEKVPRENSNDKCPMWVG
                     DKIYFLSDRAGGVVSLFSFDTKSKRVEHVVENKGLDIKYASANGDTIVYEQFGTIFTL
                     DAGSKTPKKVNIRVAGDFPGVRPRYEKVGGRIANVALSPTGIRAIFEARGEIISSPAD
                     KGDPRNLTNTTNVMERDPAWSPDGKWIAYISDEGGE"
BASE COUNT          412 a          438 c          435 g          390 t
ORIGIN      
        1 attcgccgcc ctcgtcggaa atatacgcaa tccatttgcc gtcgggcgac cacgccgggt
       61 cgcgctccat cacgttcgtc gtgttcgtca gattacgcgg gtcgccttta tcggcgggcg
      121 acgaaataat ctcgccgcgc gcttcgaaaa tcgcccggat gccggtcggc gaaagggcga
      181 cattcgcaat ccgaccgccg actttttcat aacgcgggcg cacgcccgga aaatctcccg
      241 cgacgcggat gtttactttt ttcggagttt tcgagcccgc gtcgagcgtg aaaatcgtgc
      301 cgaattgttc gtaaacaatc gtgtcgccgt ttgccgaagc gtattttatg tcgagccctt
      361 tgttttcgac gacgtgttcg acccgcttgg atttcgtgtc gaacgagaaa agcgaaacga
      421 cgccgcccgc gcggtcggaa agaaaataaa ttttgtcgcc gacccacatc gggcacttgt
      481 cgttcgagtt ttcgcgcggc actttttcga tggtcgaatc cgaaagccgg gcaatccaaa
      541 tcggctgcgt ctgcccgcct ttatagcgtt tccagtcgct ctgccattgc gaaagcggct
      601 cgtacgcgac atattgaccg tcgggcgaaa gccagccgcg gtcagccatc ggcagaggaa
      661 tttcaaacgg cagaccgctg ccgtcgaccg gaaaagtgta gaggcgcgaa gcgccgaagc
      721 cgctgttgcg cgtcgaagcg aataaaacgc ttctgccgtc cgccgtccag ccggaaactt
      781 catcgaccga cgggtgaaac gtcagccgtt tcggcacgcc gcccgtcgcc gggacgatat
      841 aaacgtcggc gtttccgtcg tattcgcccg taaaggcaat ccaatttccg tcgggcgaaa
      901 aatatggatt tgtttcgatg ccgatgcccg tcgtcaatcg acgcgcgtcg ccgcccgcgc
      961 gcgtcacaat ccacagatcg ccgccgtatg aaaaaacgat gtcggtacgg ctgacggtcg
     1021 gctgtcgaaa cagcgtcgtc tggctcgtct gcgcaaaaga cgaaacgaaa gcggcgagta
     1081 aaactaaaat gttcagaatg atttttttca cttcgatttt ctccggtaat tacacttttc
     1141 gataaatttt tgcccgagag cggcaatcgg cgcgccggtc gtgcggcaga tggctcaacg
     1201 gtctcagatt ctttattttt ctatttaatt tggataagca gaatgcctgc acggtttcgg
     1261 cagataactt caactcttca agaccgtaat tttgaaagct tttttgagtt tcggaaaata
     1321 ttccgggctc cgaatctgcc gcgccggatt tagccttaac ttccggcaat cgccgacgaa
     1381 tttactattg accgaactag atacgcacag gtttaggaaa agttatacct aaagataaat
     1441 tttcaggcaa gaaaattttt aaatggatga aaaaaaaatt aaacgaatcg ggaaatccgt
     1501 atcacgcaaa tttaataaag aagacgattc ctcgacggaa acgccttctc ctgtttgcta
     1561 aaaataaaac ggaaaggcgt gtaatcacct gttcgggtaa gcttgaacgg caaatttgat
     1621 agacgaaggc gaattgattt tgggaaaaaa agggcggcga gtagaaaacc cgccg
//
LOCUS       DASWNE010000806         2996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_275093, whole genome shotgun sequence.
ACCESSION   DASWNE010000806 DASWNE010000000
VERSION     DASWNE010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275093"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..907
                     /locus_tag="VGB68_15735"
     CDS             254..907
                     /locus_tag="VGB68_15735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050745.1"
                     /translation="MTTNNLKNFNLIFVLAIILTMTLGCSLISKLRAGSAPPTDADCS
                     NLPMGANCVTKSAPTPAIKANSEDFFESDALSEFEPKLSEKIGGEVKVLDLNVYENYL
                     KIQVQDPKKPENVDGYDYKDGVIAAPVPVRLYGGGNLEQNLFKLKEVNLARIPELIDE
                     AREKSQDLEGAKVSHVIVKKDLPFSNKVKIRIYLSGTRKSAWLVADSSGKVIEYKTF"
     gene            complement(1015..>2996)
                     /locus_tag="VGB68_15740"
     CDS             complement(1015..>2996)
                     /locus_tag="VGB68_15740"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050746.1"
                     /translation="PRFMNFGDKIELPVVVQNQTDNVMTVNVGVRATNATLTSGGGQK
                     VTVQPNDRVELRFPVSAEKAGTARFQIAATSGKFADAAEIELPVWTPATTEAFATYGT
                     TDENNAVVQPVQTPGDVFPQFGGLEITTSSTQLQELTDAFIYLYHYPYACSEQISSRM
                     ISIAALRDVLTAFKAKDMPKAEEIQKAFERDIKILQSRQREDGSFGLWKRDRERYEYP
                     FLTVHVALALSLAKAKGYKVPDEMLNKTKPYLKNIESKFDEWHRKSPQARWTISAYAL
                     YLRDMMGDKDTAKAKALLKEATLEKLPFEATGWILSVLADDKNSAAEVDAIKRFLMNR
                     TTETAATANFVTDYGDGQWLVMYSNRRADGVLLEALIKADPRNDLIPKLVRGLLAHRK
                     QGHWGSTQENVFILLALDKYFNAFEKVTPDFVTRIWLGNAYAGEEAFKGRSIDSNVLN
                     IPMSYLTEQGGISNLILDRQGAGRLYYRIGMKYAPKNLKLAPADYGFTVLRKYEALDN
                     AEDVRQNGDGSWVIKSGSRVRVRLTMVAQARRYHVALVDPLPAGLEILNPGLAVTEQI
                     PADNGGNTPVLEYGSRSIGHGSWWWRQHWFDHQNFRDERAEAFSALLWEGVYNYTYVT
                     RATTPGEFVVPPAKAEEMYHPETFGRTGTDFVRVE"
BASE COUNT          711 a          811 c          781 g          693 t
ORIGIN      
        1 gcgaagtctg gctttaccgc gtcgagcacc ggcatcaaac gccgatgaaa tattttcccg
       61 tctccttcga taccaagcgc ggcgctttcg ccggcagagt caaaagcgga accgtatcgc
      121 tactggttaa ggcataaaag aaacgcgccc tgacgaaagc cgagcgcggc gtagcgataa
      181 ttccgcgcgt atcgcctttc gctttgatgc gtttctttga ttgaaaaaac taaatcttta
      241 gaaaatataa aatatgacga caaataattt gaaaaatttt aatttaatct ttgttctggc
      301 aattatttta acgatgactt taggttgcag cttaatttcg aaattgcgcg ccggcagcgc
      361 tccgcctacc gacgccgatt gcagcaatct gccgatgggc gcaaactgcg tcacaaagag
      421 cgcgccgacg cccgccatta aagcgaattc ggaagatttt tttgaaagcg acgcgctgag
      481 cgaattcgaa ccgaagctgt ctgagaaaat cggcggcgag gtcaaagttc tggacttgaa
      541 cgtgtatgaa aactacctga aaattcaggt gcaggacccg aaaaaacctg aaaacgtgga
      601 cggctacgat tataaagacg gcgtcatcgc cgcgcccgtt ccggtcaggc tttacggcgg
      661 cggcaatctc gaacagaatc ttttcaaatt aaaggaagta aatctcgcca gaattcccga
      721 actcatcgac gaagcccgcg aaaaatcgca ggatttggaa ggcgcgaaag tgtcgcacgt
      781 catcgtcaaa aaagatttgc ctttcagcaa caaggtgaaa atcagaatct acctgagcgg
      841 aacgcgaaaa tcggcgtggc tggtcgccga ttcgagcgga aaagtcatcg aatataaaac
      901 tttctgagaa aaagcgaaac ggattttttt aaaaacattc tccgcagaaa ccaaagtaca
      961 cgaagattat taaaaatttc ttcgtgtcct ttgtgtcttc gtgctgaacc tatcttattc
     1021 gactctgacg aaatcggttc cggtgcgtcc gaaagtttcg ggatgataca tttcctcggc
     1081 tttggcgggc ggcacgacga actcgcccgg cgtggtcgcg cgcgtcacat acgtgtagtt
     1141 gtagacgcct tcccagagca gagccgaaaa ggcttcggcg cgctcgtcgc ggaagttctg
     1201 atggtcgaac cagtgttgtc gccaccacca ggagccgtgt ccgattgaac gacttccgta
     1261 ttcgagaacc ggagtgtttc cgccgttgtc agccggaatc tgctcggtga cggcaagacc
     1321 gggattcagg atttccaaac cagccggaag cgggtcgacc agcgcgacgt gataacgccg
     1381 cgcctgcgcg accatcgtca ggcgaacgcg gacgcgcgag ccggatttaa taacccacga
     1441 gccgtcgccg ttttgcctga catcctcggc attgtccaat gcctcgtatt tgcgcagaac
     1501 cgtaaatccg taatccgccg gagcgagctt cagatttttc ggcgcgtatt tcattccgat
     1561 tcggtaataa agccgtcccg cgccctgccg gtcgagaatc aggttggaaa ttccgccctg
     1621 ctcggtcaga taagacatcg gaatattcaa aacgttcgag tcaatcgagc gacctttgaa
     1681 agcttcctcg cccgcgtaag cgttgccgag ccagattcgc gtcacgaaat cgggcgtgac
     1741 tttctcgaaa gcattgaaat atttgtcgag cgccagcagg atgaaaacgt tttcctgcgt
     1801 gctgccccaa tgtccctgct tgcggtgggc aagcagcccg cgcacgagct tcggaatcaa
     1861 atcgttgcgc gggtcggctt tgattaacgc ttcgagcaga acgccgtcgg cgcggcgatt
     1921 cgaatacatc acgagccatt gaccgtcgcc gtaatcggta acgaaattcg ccgtcgcggc
     1981 ggtttccgtc gtgcgattca tcaaaaagcg ttttatcgca tcgacttccg ccgccgaatt
     2041 tttatcgtcg gcgagaaccg aaagaatcca gcccgtcgcc tcgaacggca atttttcgag
     2101 cgtcgcttct tttaacaaag ctttggcttt cgccgtatct ttgtcgccca tcatatcgcg
     2161 cagataaagc gcgtaagccg aaatcgtcca gcgcgcctgc ggcgatttgc gatgccattc
     2221 gtcgaatttg ctttcgatgt ttttcaagta aggcttcgtt ttgttcagca tctcgtccgg
     2281 cactttgtag cctttcgctt tcgccagaga caaagccaga gcgacgtgaa ccgtcagaaa
     2341 cgggtattcg tagcgctcgc ggtcgcgttt ccacaagccg aagctgccgt cctcgcgctg
     2401 gcgcgattgc agaatcttta tgtcgcgttc gaaagccttc tggatttctt ccgccttcgg
     2461 catatctttc gctttaaaag ccgtcagaac gtcgcgcaga gcggcaatcg aaatcatccg
     2521 cgaagaaatc tgctccgagc acgcatacgg ataatgataa agataaataa acgcgtcggt
     2581 cagttcctgc aactgcgtcg agctggtcgt aatttccaaa ccgccgaatt gcgggaaaac
     2641 gtcgcccggc gtctgtacgg gctgcacgac cgcgttattt tcgtcggtcg tgccgtaagt
     2701 cgcaaaagct tcggtcgtcg cgggcgtcca gacgggcaat tcgatttccg ccgcgtcggc
     2761 gaatttgccg gaagtggcgg caatctgaaa acgcgccgtg ccggcttttt ccgcgctgac
     2821 cgggaaacga agctccacgc ggtcgttcgg ctgaaccgtc accttctgac cgccgccgct
     2881 cgtcaatgtc gcgttcgtcg cgcgaacgcc gacattgacc gtcatcacat tgtcggtttg
     2941 attctggacg acgaccggaa gctcgatttt atcgccgaaa ttcataaatc gcggcg
//
LOCUS       DASWNE010000807         2118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_62912, whole genome shotgun sequence.
ACCESSION   DASWNE010000807 DASWNE010000000
VERSION     DASWNE010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62912"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(370..441)
                     /locus_tag="VGB68_15745"
     tRNA            complement(370..441)
                     /locus_tag="VGB68_15745"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(407..409),aa:Cys,seq:gca)
     gene            complement(522..596)
                     /locus_tag="VGB68_15750"
     tRNA            complement(522..596)
                     /locus_tag="VGB68_15750"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(562..564),aa:Gly,seq:gcc)
     gene            complement(692..>2118)
                     /locus_tag="VGB68_15755"
     CDS             complement(692..>2118)
                     /locus_tag="VGB68_15755"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0050747.1"
                     /translation="ASDAAADRVRNLMFNSLVRKNEKFEYVGELAKDVKIDAANLTVT
                     FTLQDNVKFHNGKDLTSADARYTLEALFKSGGYKAASFYDSVDGQKQQHITSIETPDA
                     KTLVLKVSRPALINQTLSNLVAIPIIPEGTIDQQKTAPVGSGAFKFVAFDQANNLVSL
                     EANQNYWEGAPKVQKVNVKTVTDANALQAELQSGRVDLVPNPTNISADTFNTLGKNPN
                     LQIVQGDGSNIRYIGFNVTGKPLDNVKMRQAIAYAIDREKIIRDLLSGQAKVAHSILP
                     EASWAYSAPVKYNYDPARAKQLLQEAGYKGEPIRFKFLSGSSAVSQYSQVIQSSLKEA
                     GINVEIETLEDNTLREQLKQGQFQMNTAQWIGGNQDPIFLRDLFDSEEFPDKKANGRN
                     RSRYSNPEFDKIVREAVNTTDKAKAAELYAKAQEIVAKELPYLPLWYPANMVIASKRM
                     NNIKNVNINASGDWSFVKDLTVGN"
BASE COUNT          494 a          509 c          519 g          596 t
ORIGIN      
        1 tctttatttt tgtgcaaagc cgcgatgatc agtagtgcta tctgccgaaa ttaaatggga
       61 caaacctctt cgcacctcct tagtcgatta ggactttgca aaaaacgaaa gcgtctaaaa
      121 aattagcgaa gaatttgttc aaggaagacg gaggcagaat gcttccggtt tattaacgag
      181 ccagaaatgg aagtcataat ttaatatcaa tatgaaaggc ttttccgaaa taattgaagc
      241 tgttattcag aaacccggtt ctgcattaaa agttcatctt atccggcgtt agttttattt
      301 cgtacacaat tcgtacacaa aataaaaaag ccacctttta gggtgactct aagttattga
      361 ttctattgaa ggcggcgatc ggaatcgaac cgatgaataa agcttttgca gagctctgcc
      421 ttaccacttg gctacgccgc cggaattgag aaggataaag gctaagggat aaaggcgaaa
      481 atttttatcc tttatccctt agcctttatc ctttaaaaat ttggagcggg aaacgagatt
      541 cgaactcgcg acttcgacct tggcaaggtc gcgctctacc actgagctat tcccgcacga
      601 ccgtaaatga taattctaca aatcgagaaa cttttgtcaa gccgcctgcg gaataattct
      661 tttccgggaa cttttgtcat tcgtaattta atcaatttcc gaccgtcaaa tctttgacaa
      721 agctccagtc gccgctcgcg ttgatgttga cattctttat attattcata cgtttgctgg
      781 cgataaccat atttgccgga taccagagcg gcagatacgg caattctttc gccacgattt
      841 cctgcgcttt ggcgtaaagc tcagccgctt tggctttgtc ggtcgtattg acggcttcgc
      901 ggacgatttt gtcgaattcc gggttcgagt agcggctgcg attgcgcccg ttggcttttt
      961 tatcgggaaa ctcttcggaa tcgaacaaat cgcgcagaaa aatcgggtcc tgattgccgc
     1021 cgatccattg cgccgtgttc atctgaaatt gcccctgttt taattgctcg cgcagcgtgt
     1081 tgtcttccag cgtctcgatt tccacgttga ttccggcttc tttcagagag ctttgaatga
     1141 cctgcgaata ctggctgaca gccgagctgc ccgaaagaaa tttaaacctg atgggctcgc
     1201 ctttataacc ggcttcctgc aaaagctgtt tggcgcgcgc cgggtcgtaa ttgtatttga
     1261 ccggcgcgga atacgcccac gaagcttccg gcaaaatcga atgagccacc ttcgcctgac
     1321 cgctcagcaa atccctgatg attttctcgc ggtcgatagc ataggcgatt gcctgacgca
     1381 ttttcacgtt gtcgagcggt tttcccgtca cgttaaagcc gatgtagcga atatttgagc
     1441 cgtcgccctg cacgatttgc agattcggat ttttgccgag cgtgttaaat gtatcggcgg
     1501 aaatgttggt cggattcggc acgaggtcga cgcgtccgga ttgcagctca gcctgcagag
     1561 cgttggcgtc ggtgactgtt ttgacgttta ctttttgaac tttcggcgcg ccttcccagt
     1621 aattctgatt ggcttcgagc gaaacgagat tattcgcctg atcgaaggcg acgaatttaa
     1681 acgcgcccga gccgaccggc gcggttttct gctggtcgat ggtgccttcg ggaataatcg
     1741 gaatcgccac cagattggaa agcgtctggt ttatcaacgc cgggcggctg actttcagca
     1801 ccagagtttt cgcgtcgggc gtttcgattg aggtaatatg ctgttgtttc tgcccgtcga
     1861 ccgaatcgta gaaactcgcc gccttgtagc cgccggattt aaacagcgct tcgagcgtgt
     1921 agcgggcgtc tgccgaggtt aagtctttac cgttgtgaaa cttgacgttg tcctgcagcg
     1981 taaaggtaac cgtcaaattg gcggcgtcga ttttgacgtc tttcgccaac tcgccgacgt
     2041 attcaaactt ttcgtttttc ctgaccagtg aattaaacat taaatttcgg acgcggtcag
     2101 ccgccgcgtc cgaagccg
//
LOCUS       DASWNE010000808         2827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_623401, whole genome shotgun sequence.
ACCESSION   DASWNE010000808 DASWNE010000000
VERSION     DASWNE010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623401"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VGB68_15760"
     CDS             <1..234
                     /locus_tag="VGB68_15760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050748.1"
                     /translation="EKMNLRQVPSHVNSIWVNMGAGNRDLPARLAGYNIQIRGANNAP
                     LDSDWARITVGTQQEMQVFFKALREALARSAKA"
     gene            257..952
                     /locus_tag="VGB68_15765"
     CDS             257..952
                     /locus_tag="VGB68_15765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050749.1"
                     /translation="MKIKSFLLLAAVVVLASASVFAQPLSTSSTSVWAVDFVKTRNGQ
                     QANYLKFIEQNWARARAFMKEKGIVSSYQVLSLPASEASDWDVLLMTRYKDPAAYEKR
                     EAVFNEFAEYRKTQPQTGDKINAREISEIRFNRTFTQPISSETAKMMLAVQTTDAELA
                     AARIPLEIYIKAHAGGDGGNLDKSFYPEAKIQGIAPADGKLISYAFKDYVKTFPGKPA
                     TDEAARTRRNERL"
     gene            1540..2703
                     /locus_tag="VGB68_15770"
     CDS             1540..2703
                     /locus_tag="VGB68_15770"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY0050750.1"
                     /translation="MKRVFLLITLLAAFSVAGLSQINQASQTEKSVREFADVADEIKF
                     YLTEAGYYESSGCLVYPDQKQLARVRVLASENKDLFLSMAGNELKNAPVEKTKYVVTV
                     LTYAGTEDNRPLMNLLTPLNLKTVFAQNANAILEQLNYHLKFGHDFTEKLYVIESLVA
                     LKHYAAVPEIERQLNEESNPHLGKMMKLLIDLNAGGEIFEFVQTKILNDLCNKRGYSV
                     DYGVKVKTVLETLQALSEEADSVYAASFEKIRRRIVFEPDEASLIKFANQSKSEQLRR
                     DAVLLLAVSKTDAGLKVILKRMKDPSNMVRMAAVQSLMVYPNPKEEAVEALITVYQTD
                     ADLYIRHSSIATLGTLGGNRAEEIIREAVNDKSPMIRELAQRIIGNLEKAENR"
BASE COUNT          783 a          677 c          710 g          657 t
ORIGIN      
        1 gaaaagatga atctgcggca agtgccgtcg cacgttaact ctatctgggt gaatatgggc
       61 gcgggaaaca gggatttgcc ggcgcggctc gccggataca acatccagat tcgcggcgcg
      121 aacaacgccc cgctggattc ggactgggcg cgaatcacgg tcgggacgca gcaggaaatg
      181 caggttttct tcaaagccct gcgcgaggcg ctggcacgga gcgcgaaagc ttaattttag
      241 aaacaacaaa accattatga aaatcaaatc gtttctttta ctcgccgccg tcgtcgtttt
      301 ggcgtcggcg agcgttttcg ctcagccatt atcgacctca tccacttcgg tctgggcggt
      361 cgatttcgtt aagaccagga acggtcagca ggcgaattac ctgaaattta tcgaacagaa
      421 ctgggcgcgg gcgcgagcct ttatgaaaga aaaaggcatt gtttcgagct atcaggtgtt
      481 gtctctgccg gcgagcgagg cgagcgattg ggacgttttg ctgatgaccc ggtataaaga
      541 cccggcggct tacgaaaagc gcgaagccgt ctttaacgag ttcgcggaat accgtaaaac
      601 tcagcctcaa accggcgaca agattaatgc gagggaaatt tccgaaatca ggtttaaccg
      661 gacatttact cagccaatct cgtcagaaac ggcgaaaatg atgctcgccg tccagacgac
      721 cgacgcggaa ctggctgccg cgcgcatccc gctcgaaatt tacataaagg cacacgccgg
      781 cggcgacggc ggcaatctcg ataaatcttt ctaccccgaa gctaaaattc agggaatcgc
      841 cccggcggac ggtaaattga taagttacgc gttcaaggat tacgtcaaaa cgtttcccgg
      901 caaaccggcg accgacgaag cagcgcgaac gcgccgcaac gagagactgt aaatcacgaa
      961 aaacgccgcc gtcgccaagc tcgtcttcga ttacccttca ataaaaatca ccgattattt
     1021 gctgcttctg aaaatcgacg gcgaatggaa aatcgtcaat aaaatcgcgc agggcgagcc
     1081 gaaaacgccc gccccgaaat aaagtgcata aatatatatt gaattacttt ggatgattaa
     1141 aagatattaa tcgtcgatgt cttcgcattg ttaaactgtt acgcggtcaa ttttaatcca
     1201 aacaatgaag gtaacttaaa atgatacaga acaatcgatt tcgctccaca ggcaaacatt
     1261 tatcatacgg tcttgcgcgt gtatttttca tcacactttt tttgctttta tgtcagaacg
     1321 cgcgcaccgc tcagctcagc ttttgaaaaa accgaatcag gttgccgtta ttgtccgcga
     1381 ttatgaaaag aacggttcga aaccgtttaa cagcccgttt cagccggttt ggctatcttt
     1441 aaattactta tcgaaaaatc tcgacggagc ccgggttcat ccgaaattat taatcctcgc
     1501 cgaaacaacc tttcttcgat tcggctcaac atatatttta tgaaaagagt cttcctttta
     1561 atcaccctgt tagccgcgtt ttccgtagcc ggtttatctc aaataaacca ggcgagccag
     1621 actgaaaaat cggttcggga atttgccgat gtcgcggacg aaatcaaatt ttatctgacc
     1681 gaggcgggtt attatgagag cagcggctgt ctggtttatc ccgaccagaa gcagctcgct
     1741 cgcgtgcgcg tgctggcgag cgaaaacaag gatttgtttt tatcgatggc gggtaatgaa
     1801 ttaaaaaacg cgccggtcga gaagacgaaa tatgtggtga cggttttgac ctatgccgga
     1861 acggaagata atcgcccgct tatgaatttg ctgacgccgc tgaacctgaa aaccgttttc
     1921 gcgcaaaacg ccaatgcgat tctggaacaa ttaaattatc acctgaaatt tggtcacgac
     1981 tttactgaga aactttacgt gattgagtcg ctggtcgctt tgaaacatta cgcggcggtg
     2041 ccggaaatcg agcgccagct caacgaagaa agcaatccgc atctcggcaa aatgatgaag
     2101 cttttgattg atttaaatgc gggcggcgag attttcgagt ttgtacagac taaaattttg
     2161 aatgatttat gcaacaaacg cggctattcg gtagattacg gcgtgaaggt aaaaactgtg
     2221 ttggagacgc ttcaggcgct ttcggaagaa gccgattcgg tttacgccgc atcgtttgag
     2281 aaaatccgcc gccggattgt tttcgagccg gatgaagcgt cgctgataaa atttgccaac
     2341 cagtcaaaaa gcgaacagct gcgcagagac gccgttttat tgctggcggt ttctaaaacc
     2401 gatgccggat taaaggtcat tctcaaacgt atgaaagacc cgagtaatat ggtcagaatg
     2461 gcggcggtgc aatctctgat ggtgtatccg aacccgaagg aagaagcggt cgaggctttg
     2521 attacggtct atcaaacgga cgcagacctc tatattcggc attccagcat cgccacgctc
     2581 ggcacgctcg gcggcaaccg cgccgaagaa ataattcgtg aagccgtcaa tgacaaaagc
     2641 ccgatgatcc gcgaactggc gcagagaatc atcgggaatc tggaaaaggc tgaaaacagg
     2701 taaaatccgg aaatattttg ccgaaaaccg gctcgaaagc cgcaagcggc aaaggatttc
     2761 aaaaacggca attacttaaa gaacgttttt tttccttgcg ggcggattct tccccgccgc
     2821 gcgcgac
//
LOCUS       DASWNE010000809         9309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_166364, whole genome shotgun sequence.
ACCESSION   DASWNE010000809 DASWNE010000000
VERSION     DASWNE010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9309
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166364"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..1352)
                     /locus_tag="VGB68_15775"
     CDS             complement(96..1352)
                     /locus_tag="VGB68_15775"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY0050751.1"
                     /translation="MKNLKVLIALVVVGLLLNPTIAPAQTEIRFDKGNFETAIRRNFN
                     GRVMGYQVILLKKGQIVSEMADGLARNPADGNVKMTVDTPANIGSTAKFFGGTALLQI
                     FQKTNEMDSWLGKEVYKFFPKVWQDEMDDSIKQIRFRDLLQHEGGFIHNDPDPTIKVY
                     FDYLRKGVTSDQSQTYAYGKRNYANANITTIGYVLAAVNNPLFLNMVNKKIADQNLKA
                     NDPAIEALLGQGFENYMKAKIFSQINPAISPSCDAPNEYPKKNIVYAKMYALPTTATP
                     GAEFSSKAKIGACHAAGGWYVTGRELATYVANYAASDEIVTKPTRDLMFDDDDAKERL
                     VWSMVEPDGSLLKNFGWNTSPYMGGDHPVGSSKAHATIVKLPNDYYAVGIINSDILNT
                     QGQPGGSRLLTQNIIEAFKAAVAANF"
     gene            1591..2151
                     /locus_tag="VGB68_15780"
     CDS             1591..2151
                     /locus_tag="VGB68_15780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0050752.1"
                     /translation="MVSENQNVTELLKKWSGGDAAALEELTPLIYAELHRLAKSYMRR
                     ERDGHTLQTTALVNEAYLRLIDWKAVKWENRAHFFGVSANLMRKILVDFARRRPKSKD
                     GKMQQVSLDEAFKITARSADLVALDEALNKLAAWDERKAKIVEMKFFGGLSVEETAEV
                     LKISGVTVMREWSRAKAWLYRELSQA"
     gene            2155..5043
                     /locus_tag="VGB68_15785"
     CDS             2155..5043
                     /locus_tag="VGB68_15785"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0050753.1"
                     /translation="MNAARYRQIDEIFAEALEIPDAAREAFLSERCGGDLELKTEVLS
                     LLKAQTGAEEFMENSAMNLLARELAEENPDAHLSLVGRQFGTYKIEKPIGAGGMGEVY
                     LALDSKLNRKVALKILPAEFLTDTERVKRFEREALAVSALNHPYIVTIHDVGSADGIN
                     YIATEYVEGETVRDLINRGAQFKQTLSVISQTCEALVAAHKAGIIHRDIKPENIMIRP
                     DGYVKVLDFGLAKLVEDTEFQQSLSNYTQKGIIIGTPAYMSPEQVGDDRVNHRTDLWS
                     IGVCLYEMLTGVNPFKGESRQSTFQKILSAEPPLVSELKPNLPAELDRILSKALEKDR
                     DVAYQTASELRADLKRVRREIDSSPSLESAGGTRTDKAAEKTRKNYLIPAFGVLLLTL
                     IGAGVWFFVFRQNRSVAIDWSKATNIQLTDRQGTEFFPALSPDGKTFVYASNAGGKFD
                     VFIQRIGGKNPTNLTKDSPADDTQPAFSPDGEQIAFRSEREPKGIYIMGASGENQRRV
                     ADFGFQPSWSPDGKEIVVGTFGLAAPNVRIGHNNGLWIVNVETGAKRELLKENATFPA
                     WSPNGKRIAYWYYPETFARRDIATIPASGGEPVVITKDFATSNWNPVWSPDGRFLYFV
                     SDKNGNLNFWRVSIDETTGEALSPPEPIVSPSKYSRHLNFSRDGKQMIYVQTDIQSNI
                     QGVEFDSPNERAVGDWFWITRGDRVISRAELSPDGKQFVMRQARRTQDDIVVVSRDGS
                     DWRDITSDAPFDRFPRWSPDGSQIAFASDRNGGMEIWVCDADGSNMRQVTFDYTPRLG
                     SSFPNWSPDGKQLIYTNDSQSWLIDLTKNWEQQSPQRVLPPEIENRFITWDWSPDGKK
                     IVGVFPHEKYAIGFYSLETKRLERLSDETADGETTQSWLADSRRFVFARENKIYIADT
                     ETKKIKEIFSHQPEDIRTPFVSRDGRLLYYTAHSSQSDIWLLDNSQNQ"
     gene            complement(5169..6308)
                     /locus_tag="VGB68_15790"
     CDS             complement(5169..6308)
                     /locus_tag="VGB68_15790"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY0050754.1"
                     /translation="MKASIMEDLQKLKTPTLVLDIERVRRNAQRISRIAKTNAVGLRP
                     HIKTHKCIEIAQIQTDGHGGAITASTLAEVRAFSKHGFNDITYAVPIEPGKFDEAIEI
                     AGSGVKLNLITDSPDIPPLLNNDLRRAAVRMNVFLKVDCGSHRCGVEPHSDEAFEIPR
                     LIANASNLNFAGILTHAGHSYSARSEEELAAVARHERDSMTELAARLESAGIEVPIVS
                     IGSTPTITHIDHLKGIDEIRPGNYILFDASQAALGSCDFEDCALTVLAAVIHIDRGRK
                     RVVLDAGAIALSKDRGPVGLDSTCGYGRVLDLEGNETGLRLESLSQEHGVFHVGDDAV
                     LERLRVGTRMRVLANHSCLTAAQHPFYNVLENGRIIDKWKIHYGW"
     gene            6827..7054
                     /locus_tag="VGB68_15795"
     CDS             6827..7054
                     /locus_tag="VGB68_15795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050755.1"
                     /translation="MADDKDKTAYIGIERGENSLKYIAKVFDEPRREITKQQYNAIVK
                     ALGKQDWKNSEIKDESSIISTIISSQLNPPA"
     gene            7439..8596
                     /locus_tag="VGB68_15800"
     CDS             7439..8596
                     /locus_tag="VGB68_15800"
                     /inference="COORDINATES: protein motif:HMM:NF044863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050756.1"
                     /translation="MNLGLGSSVYPAAKMVSARLEKYFSSHFSQLPVRNNGEFASVPD
                     VLTIEKMIDTAFWASLQREEGYAPKISLAYLPSEQAGQPMMFEESLPLDSHALIKLSP
                     AVERPSIYLGVWRDASNQLSVWGATRTVPQNCFVLEVVEPGLLVVKHRTKTGQGKFAN
                     VLILSGEQIKEIDEKGTSLADCPDILASMLRFGSSDSSDDSVGVLIQLAVSMREHKRG
                     GSLLIVPQKSETWRESIVLPARYSVTPSYTGLADLIRRPPGSERDERLHQEMLFDAVE
                     AIAGLTAVDGATVISDEFELRAFGAKIKRRDAAPQVEQTIITEPIIGNRASVVHPLQF
                     GGTRHLSAAQFVQDQPDSVALVSSQDGRFTVFAWSPCEAMVHAHRIESLLL"
     gene            complement(8697..8837)
                     /locus_tag="VGB68_15805"
     CDS             complement(8697..8837)
                     /locus_tag="VGB68_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050757.1"
                     /translation="MPSKRRKIYEAEPKSGAKAKPAFYINEIKRGSSYELGVLPKAIA
                     RG"
     gene            complement(8878..>9309)
                     /locus_tag="VGB68_15810"
     CDS             complement(8878..>9309)
                     /locus_tag="VGB68_15810"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0050758.1"
                     /translation="FGASGDKPVAADYDGDGKADYAVYRPSDGTWYLLRSQQGFTGVQ
                     FGIATDKPVVGDYDGDGKVDPAVYRDGIWYQLRSSQGFTAVQFGIQTDLPAPADYDGD
                     GKTDIAVYRDNSWYLQQSTQGFNGFQFGAIGDKPVPNAFVP"
BASE COUNT         2280 a         2446 c         2368 g         2215 t
ORIGIN      
        1 gagaaaaaca gaccgccggt gacgcggctg aaatcagtga aaatctgatt tgtccgtttc
       61 accggcggtc tgtttttttt gcgcattacc tgccgttaaa aatttgcggc gacggctgcc
      121 ttaaacgctt cgatgatatt ctgggtcaaa agcctcgaac cgccgggctg cccttgcgtg
      181 ttcaaaatat cggaattaat gattccgacg gcgtaataat cattcggcag cttgacgatc
      241 gtggcgtgcg ctttgctgct tccgaccggg tggtcgccgc ccatataagg cgaagtgttc
      301 cagccgaaat ttttcagaag actgccgtcg ggctcgacca tagaccaaac cagacgctct
      361 ttcgcatcat cgtcgtcaaa catcaaatcg cgcgtcggtt tcgtaacaat ctcgtcggat
      421 gcggcgtaat ttgccacata cgtagccagc tcgcgccccg taacgtacca gccgccagcc
      481 gcgtgacacg ctccgatctt ggcttttgaa gaaaattcgg ctcccggcgt agcggtcgtc
      541 ggcagagcat acatttttgc gtaaacgatg tttttcttcg ggtattcgtt cggcgcgtcg
      601 cagctcggcg aaatcgccgg atttatctgg ctgaaaattt tcgctttcat ataattttca
      661 aaaccctgac ctaataaagc ttcaattgcc gggtcattgg ctttgagatt ttggtcggcg
      721 atttttttat tgaccatatt taaaaacaac ggattattaa ccgccgccag cacataaccg
      781 atggtcgtaa tgttggcgtt ggcatagttt cttttcccgt aggcgtaagt ttggctctgg
      841 tcgctcgtca cgccttttct caggtaatcg aaataaactt taatagtcgg gtcggggtcg
      901 ttgtggataa agccgccttc gtgctgaagc aaatcccgaa acctgatttg cttgatgctg
      961 tcgtccattt catcctgcca gactttggga aaaaacttgt atacttcttt gcccagccaa
     1021 gagtccattt cgttcgtttt ttgaaatatt tgcagcagcg ccgtgccgcc gaaaaatttc
     1081 gccgtgctgc cgatattggc gggcgtgtcg acggtcattt tcacgttgcc gtccgccggg
     1141 ttgcgcgcca gaccgtccgc catttcggaa acgatttgac cttttttcag caaaatgacc
     1201 tgatagccca tcactctgcc gttgaaattc cttctaattg ccgtttcaaa atttcctttg
     1261 tcgaatctga tttccgtctg tgccggagcg attgtcggat tgagcagcaa cccgacaact
     1321 accagcgcga ttaaaacttt tagatttttc ataattatct ccttcgtata aattcggcgc
     1381 gttttccgcc gttcgatagc tatggcgtcg gagttcgcca aaacctatca cgccggagaa
     1441 aaaatatttt tcgttttgcc gcgactgtgt taaatttgaa ttcttaattt ttgccgaaac
     1501 ccgcgcgaaa tcaatgtctg accgcgataa ataagacatt tgctacgtgc ctgttccggg
     1561 catttttgac tttcgaattc gtaggcgaaa atggtttctg agaatcaaaa cgtaaccgag
     1621 ctcctcaaaa aatggagcgg cggcgatgct gccgcgctcg aagaattaac gccgttgatt
     1681 tacgccgagc tgcaccgcct agccaaaagc tatatgcgcc gcgagcgcga cggacacact
     1741 ttgcagacga ccgcgctggt caacgaagcc tatcttcgcc tgatcgactg gaaagccgtc
     1801 aaatgggaaa atcgggcgca ttttttcggc gtttcggcaa atttaatgag aaaaattctg
     1861 gtcgattttg cccgccgccg accaaaatcg aaagacggca aaatgcagca ggtttctttg
     1921 gacgaagcct ttaaaataac cgccaggtct gcggatttag tcgcgctcga cgaagccttg
     1981 aacaaactcg cggcttggga cgagcgcaag gcaaaaattg tcgagatgaa atttttcggc
     2041 gggctttcgg tcgaagaaac ggctgaagtg ctgaaaatct ccggcgtcac ggtaatgcgc
     2101 gaatggtcga gagcaaaagc gtggctctac cgcgagttaa gccaggcata aaggatgaat
     2161 gctgcccgtt acagacaaat tgacgagatt ttcgccgagg cgctggagat tccggacgcc
     2221 gcgcgcgaag cgtttttgtc cgaaaggtgc ggcggcgacc tcgaattaaa gaccgaggtt
     2281 ctgagtttat taaaagccca gaccggcgcg gaagaattta tggaaaattc ggcgatgaac
     2341 ctgctggcgc gggagctcgc ggaagaaaac ccggacgcgc atctttcgct ggtcggcagg
     2401 caattcggca catataaaat agaaaagcca atcggcgcgg gcggcatggg cgaagtttat
     2461 ctggcgctcg attcgaaatt aaaccgcaag gtcgcgctga aaattttgcc cgcggaattt
     2521 ctgaccgaca cggagagagt taaaagattc gagcgagaag cgcttgccgt ctccgccctg
     2581 aatcatccgt atatcgtgac gattcacgac gtcggaagcg cggacggaat caattatatc
     2641 gccaccgaat acgtcgaagg cgaaaccgtc agagatttga taaatcgtgg cgcgcagttt
     2701 aaacagacgc tttcggtcat ctcgcagacc tgcgaagcgc tggtcgccgc gcacaaagcc
     2761 ggaatcattc accgcgacat caagccggaa aatataatga ttcgccccga cggttatgtc
     2821 aaagttctgg atttcggtct ggcaaagctg gtcgaagata ccgaatttca gcaaagtctc
     2881 tcgaactaca cgcaaaaagg catcattatc gggacgcccg cctatatgtc gcccgaacag
     2941 gtcggcgacg accgcgttaa ccatcggacg gatttgtgga gcatcggcgt ctgtttatac
     3001 gaaatgctga cgggcgtcaa tcctttcaag ggcgagagcc ggcaatcgac gtttcaaaaa
     3061 atcttatccg cagagccgcc gcttgtttcc gagctgaagc ctaatcttcc cgccgagctc
     3121 gatagaattt taagcaaagc tctggaaaag gacagggacg ttgcttatca aaccgcgtcg
     3181 gagcttcgcg ccgatttgaa gcgcgttcgc cgcgagattg attcgtcgcc ttcgctcgaa
     3241 agcgccggcg ggacgcgcac ggacaaagcg gcagagaaga cgcggaaaaa ttatctgatt
     3301 ccggcgttcg gcgttttact tttaacttta atcggggcgg gcgtctggtt tttcgtattt
     3361 cggcaaaacc ggagcgtcgc gattgattgg agcaaagcga cgaacattca actgaccgac
     3421 cggcagggga cggaattttt tccggcgctc tcgcccgacg gaaaaacctt tgtttatgct
     3481 tctaacgcgg gcgggaaatt cgacgttttt attcagcgca tcggcggtaa aaacccaacg
     3541 aacttaacca aagattcgcc agccgacgac acgcagcccg ctttttcgcc ggacggcgag
     3601 caaatcgcct ttcgctcgga gcgcgagccg aaaggcatct atattatggg cgcgtcgggc
     3661 gaaaatcagc gccgggtcgc cgatttcggt tttcaacctt cctggtcgcc cgacggcaag
     3721 gaaatcgtcg tcggaacttt cgggcttgcc gcgccgaacg tgcgcatcgg acacaataac
     3781 ggcttgtgga ttgtcaacgt cgaaaccgga gcaaaacgcg agctgctcaa agaaaacgcg
     3841 acgtttcccg cctggtcgcc gaacggaaag cgcatcgcct attggtatta tccggagact
     3901 ttcgcccgcc gcgacatcgc cacgattccg gcaagcggcg gcgaacccgt cgttattacc
     3961 aaagattttg ccacgtcaaa ctggaatccg gtctggtcgc cggacggcag gtttttgtat
     4021 ttcgtttccg ataaaaacgg aaacctgaat ttctggcgcg tttcgattga cgaaacgacc
     4081 ggcgaagctc tttcgccgcc cgaaccgatt gtttcgccct caaaatacag ccgccatctg
     4141 aatttttcgc gcgacggaaa acaaatgatt tacgtgcaga ccgatattca atcgaacatt
     4201 cagggcgtcg aattcgattc gccgaacgag cgagccgtgg gcgattggtt ttggataacg
     4261 cgcggcgacc gggtgatttc gcgggcggag ctctcgccgg acggcaagca gtttgtgatg
     4321 cggcaggcgc ggcgcacgca ggacgacatc gtggtcgtca gccgtgacgg aagcgattgg
     4381 cgcgacatca ccagcgacgc gccgtttgac cgttttccgc gctggtcgcc ggacggcagt
     4441 caaatcgcct tcgcctcaga ccgcaacggc ggaatggaaa tttgggtttg cgacgccgac
     4501 ggcagcaata tgcggcaggt cacttttgat tatacgccca ggctgggctc gtcatttccc
     4561 aattggtcgc cggacggcaa acaattgatt tacacgaacg actcgcagtc gtggctgatt
     4621 gatttaacaa aaaactggga gcagcaatcg ccgcagagag ttttgccgcc ggaaatcgaa
     4681 aaccgattta tcacgtggga ctggtcgccc gacggcaaaa aaatcgtcgg tgtttttccg
     4741 cacgaaaagt atgctatcgg cttttattcg ctggaaacaa aacgtctgga acgtctctct
     4801 gacgaaaccg ccgacggaga aacgacccag tcgtggctcg ccgacagccg ccgcttcgtg
     4861 ttcgcgcgcg aaaataaaat ttacatcgcc gacaccgaaa caaaaaaaat aaaggaaata
     4921 ttttcacacc agcccgaaga tattcgcacg ccgttcgtca gccgcgacgg gcgtctgctt
     4981 tattacaccg ctcattccag tcaatccgac atctggctgc tcgataattc tcaaaatcaa
     5041 taaaaaagta agattgccag gatatatttc ataatttgct ttgattaaag cttataaaat
     5101 gtattttatt ttcgttttta atctgtaatc gaaaaactca aaaacgattc tttgcgagcc
     5161 ggtttctctt accagccgta atgaatcttc cacttatcga taatccgccc gttttcgagc
     5221 acgttataaa agggatgttg cgccgccgtc aggcacgaat gattcgccag aacacgcatc
     5281 cgggttccaa cccgcaaacg ctccagtacg gcgtcatccc cgacgtgaaa gacgccgtgt
     5341 tcctgagaaa gcgattcgag ccgcagtccc gtttcatttc cttccaaatc gagcactcgc
     5401 ccgtaaccgc aggtcgaatc gagcccgacc ggacctctgt ctttggaaag cgcgatcgcg
     5461 ccggcatcga gaacgaccct ttttctgccg cggtcgatat gtattaccgc cgccaaaacc
     5521 gttaaggcgc aatcttcaaa atcacagctt cccaaagcgg cttgcgaggc gtcgaacaaa
     5581 atgtaattgc cgggacggat ttcgtcgatg cctttcagat ggtcgatgtg cgttatcgtc
     5641 ggcgtcgagc cgatgctgac gatcggcact tctattccgg cgctctcgag ccgcgccgcc
     5701 agttccgtca tcgaatcgcg ctcgtgtcgc gcgacggcgg caagttcctc ttccgaacga
     5761 gcgctatagg aatgtccggc gtgagtcaat attccggcga aattcaagtt tgaagcattt
     5821 gcaatcagac gcggaatttc aaaggcttca tcggaatgcg gttcgacgcc gcagcgatgc
     5881 gaaccgcaat cgactttgag aaaaacattc atcctgacgg ctgcccgccg aagatcgtta
     5941 tttagaagcg gcggaatatc cgggctgtcg gtgatgagat tgagcttaac gccgcttccg
     6001 gcgatttcaa tcgcttcgtc aaactttccc ggttcaatcg ggacggcata agtaatatca
     6061 ttaaagccgt gttttgaaaa cgcgcggact tcggcaagcg tcgaagccgt aatcgcgccg
     6121 ccgtgaccgt ctgtttgaat ttgcgctatc tcgatgcatt tatgagtttt aatatgcggg
     6181 cgcagcccga ccgcgtttgt tttcgctatc cggctgattc tttgcgcatt gcgccgaacg
     6241 cgctcgatgt cgagcaccag cgtcggagtt ttcagttttt gcaagtcttc cataatcgag
     6301 gctttcaaaa agaaacgtgt aattaccgat tataattctc tatctaatgt aacaaactta
     6361 attttaagtt ttcccgaaag aatgaaatat tacccgcctt caggcttacc cgaaaatcaa
     6421 gcagtcaaaa attattctca tttcagcggc ggaaatcaaa tgccgtcgaa gcctgtttga
     6481 aatttcagga ttgtcgtctg aatatccgaa aaaatcaaac aatctcaaac cggtttttat
     6541 gtctgacgac caatcttaaa caaaactctc cgccgggatg ttccggtgat tcaacaaaga
     6601 cgcgatttca atccgaaacg cgccgtctga attttagctt tgtcctcgat tgcccggaca
     6661 acataaaagg cttttatcga cgcccgataa aattttagcg ccgcaccaaa aaactgttta
     6721 caaattcttc acaatcggtt aaaaaggaag cgaacaaaat aaaatccgat tttttcagta
     6781 tcaagcctta aaccgtaata tggcggatgt tcgaagagta taagcaatgg cagacgataa
     6841 agacaaaaca gcatatatcg gtatcgaaag aggagaaaat tctctgaaat acatcgcgaa
     6901 ggttttcgac gagccgcggc gagaaattac caaacagcaa tacaatgcca tcgttaaagc
     6961 gctgggcaaa caagattgga aaaatagcga gattaaagac gaatcgagca tcatcagcac
     7021 catcatcagc agccagctaa acccgcccgc gtaaatttcc gggcgaatcg aacggtaatg
     7081 ccgccgggct ttcgcgtccg cgcgcgcgtg atgagcttcg cctttctcta tcgctcttgc
     7141 cggcatttat gaaataaccg taatttgacc cggatgagaa tcgggcggct tacgaaacgc
     7201 cgaatgccgg agactcgtcg gcttatcaat atgcttatcc gcgccggatg gagattttaa
     7261 catggaaaaa agatacgaaa tttttgtcag cttgacctcc ggcgaaaaat agataatata
     7321 catcggtccg gcgtaattta agaacgacaa aagcgggaga aaacgtcttt acaacataaa
     7381 aaaatcaaat aaactcgccg tcttgataaa acggcgagtt tagtaaaaac tgcttgttat
     7441 gaatctcggc ttagggtcat ccgtctatcc ggcggcaaag atggtttccg ccagactgga
     7501 aaaatatttc tccagccatt tcagtcaatt accggttcga aacaatggcg agtttgcgtc
     7561 cgttcccgac gtgctgacaa tcgagaagat gattgatacg gctttctggg cgagcctgca
     7621 gcgtgaggaa ggttacgcgc cgaaaatctc gctggcgtat ctgccgtccg agcaagccgg
     7681 tcagccgatg atgtttgaag aatcgctgcc gctcgattct cacgcgctga ttaagctttc
     7741 accggcagtc gagcgcccga gcatctatct cggcgtgtgg cgcgacgcct cgaatcagtt
     7801 gtcggtctgg ggcgcgacgc gcacggttcc gcaaaactgt ttcgtgctgg aggtggtcga
     7861 accgggtttg ctcgtcgtca agcaccgcac taaaaccggt cagggcaaat tcgccaatgt
     7921 tttgatactg tccggcgaac aaataaaaga aattgatgaa aaggggacga gtctggcgga
     7981 ttgcccggat attctggctt cgatgcttcg cttcggctcg tccgattctt cggacgattc
     8041 ggtcggcgtt ctcatccaat tggcggtctc gatgcgcgag cataaacgcg gcggctcgct
     8101 tcttatcgtt ccgcaaaaat cggaaacctg gagagaatcg atcgttttgc ccgcgcgcta
     8161 ttcggtgacg ccttcttata ccggactcgc cgacctcatc cgccgcccgc ccggcagcga
     8221 aagggatgaa agactgcatc aggaaatgct tttcgacgcg gtcgaagcga ttgccggact
     8281 gacggcggtc gacggcgcga ccgttatcag cgacgaattc gaattgaggg ctttcggagc
     8341 taaaatcaaa cgtcgcgacg ccgccccgca ggtcgaacag acgataatca ccgagccgat
     8401 tatcggcaac cgggcttcgg tcgtgcatcc gctccaattc ggcggcacga ggcatctgtc
     8461 agccgcccaa ttcgttcagg accagccgga ttcggtcgct ctggtctcct ctcaggacgg
     8521 tcgctttacg gttttcgcct ggtcgccctg cgaagcgatg gttcacgcgc accggattga
     8581 atcgctgctg ctctaaagtc agccgcgatt gcagtcaacc cgggtcaatc cctaggttta
     8641 tccgcaataa aatcagacat tcctgctaaa caattatgca gcaatccgct gcaagctcag
     8701 ccgcgcgcga ttgctttcgg caaaacgccc aattcgtatg acgagccgcg tttgatttcg
     8761 ttgatataaa acgccggctt tgccttcgcg ccgctctttg gctcggcttc gtaaatcttc
     8821 cggcgctttg acggcatata atcttcggtc gattctccgt aattctgctt attttactta
     8881 cggcacaaaa gcgttcggca cgggtttatc gccgattgct ccaaactgaa aaccgttgaa
     8941 gccctgagtc gattgctgca aataccagga attatcgcga taaacggcga tatcggtttt
     9001 cccgtcgccg tcgtaatcgg cgggcgcggg caaatccgtc tggattccga attgaacggc
     9061 ggtaaatccc tgactgcttc tcaattgata ccagattcca tcgcgataca ccgccgggtc
     9121 gactttgccg tcgccgtcat aatcgccgac aacaggctta tcggtcgcaa tgccgaactg
     9181 cacgcctgta aatccctgct ggcttctgag cagataccac gttccgtccg acggtcgata
     9241 aacggcgtaa tcggctttgc cgtcgccgtc gtaatccgcc gccaccggct tatcgccgct
     9301 tgcgccgaa
//
LOCUS       DASWNE010000810         5498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_97311, whole genome shotgun sequence.
ACCESSION   DASWNE010000810 DASWNE010000000
VERSION     DASWNE010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5498
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97311"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..3125
                     /gene="bamA"
                     /locus_tag="VGB68_15815"
     CDS             24..3125
                     /gene="bamA"
                     /locus_tag="VGB68_15815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEY0050759.1"
                     /translation="MHILRAFGLVLLSFVLSAVPVAAETDDKANENPKTVSNSNQPKP
                     QQLVEEVDIQGNRRLRDEDLLYYIKTRAGDVFNPQQLERDLRELLSLNFFDKTATRVL
                     TEEGVRGGVKVIFEVQELPIIRDLRFEGNEAVPESEILKQFREQRVGISKEAVFDPVK
                     VNAAKRVIRELLASKGFPNATITEKIETVSATSVAVTFEVEQGRRSRIVDIQFEGNEV
                     FKDGELRNALQLVRETGLISRFQGQDILDLRKLQYDLQKNVRDYMFSKGYFQARIGEP
                     QVEGLGVRRTDFIPLITLPLPLISSKDDTLRIIVPVTEGKLYRVGDIKVEGNSIFSEQ
                     QILNGLGLRKGEVADGQRLRKALFEDLKKAYGSQGFVLYDAELDPQFKDNPANPNEGI
                     VDINIAIDEGKQFRLRRLEFAGNTFTRDRVLRREVLINEGDIYNQLALETSIIRLNQT
                     QYFDPLDKDKDVEIRTDEETGDVDLIVNVKEKGRQQISFNGGISGIGGSFFGLEYSTN
                     NLFGRGEVFAFNAGFGNRQTTLQLSFTEPYFRDRPISIGFSLFASRYKFFGEGTFLSQ
                     NQDLFFDAQNPFGTVLTDEDNLFTQTTYGASVFATAPLSEFLPKRRFSQFSRVGLTYQ
                     FSATSIKDPPVNEDGNAATEIPVVFRQPNITTSRITPSFVYDSRQPSANGIDTLRGTQ
                     LAVSVGFAGLGGDVRTYQPNLTFTHFVPVRRKRSPNAEVFGFRIQAGTIGAWSISNAV
                     NSANSLAFVNGVPFTERYFLGSEFDLRGYNVRAIGPIAPVDTFITSQNVVVATNPSGT
                     PTPITGLPPERVAEIAALGTFTGSGGANPGLISQSFTYIGGDTQLLGNFEYRVPIFGP
                     ISLAAFADIGSVFNLRKGKTQAINSAFLQDQPFLGAFNSLNIVFLRNNTQFQPSFSGG
                     LLLRDGRPLTRSEFQQIYCGNSPTTCPLTVPAGLQEVFLRGDAQTNQLVRVDESRFAK
                     LGDFRSSVGLELRVQVPVVNVPFRLIYYFNPNAKIGFTEELPNFFLPGKRNGLRFSVG
                     RTF"
     gene            3235..3849
                     /locus_tag="VGB68_15820"
     CDS             3235..3849
                     /locus_tag="VGB68_15820"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEY0050760.1"
                     /translation="MKTFRSIAVSLFFAALFTVSAFAQTTPTQPAAGRIVTINTAMFE
                     DSKAGITKYVNAMTALNNEFEPAYKELQTMNTRLQTLQKQYNDLYTEAQKPGSPIKQE
                     ALQAKLDEIQNLQVEFKRRQEDAKIKFERRQAVVMGPVMQDIMKAISEFTKQKGYGLV
                     LDAAKLDNAGIILGWDEAKVDVTKEFVVFYNARPATTAVTTPKP"
     gene            3934..4374
                     /gene="fabZ"
                     /locus_tag="VGB68_15825"
     CDS             3934..4374
                     /gene="fabZ"
                     /locus_tag="VGB68_15825"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEY0050761.1"
                     /translation="MTVLDSRAIQEILPHRYPFLLVDKIIELVPRERIVGIKQVTINE
                     YFFRGHFPEAPVMPGVLIIEALAQVGAILALREFDDRSKKIPFFSGIENARFRRPVVP
                     GDTLTLEVVPLRMGSKVQKMRGLARVDGQLAAEAEIMSVIADRG"
     gene            4461..5237
                     /gene="lpxA"
                     /locus_tag="VGB68_15830"
     CDS             4461..5237
                     /gene="lpxA"
                     /locus_tag="VGB68_15830"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR01852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEY0050762.1"
                     /translation="MTFIHQTAIVSPQAVIGDDCYIGAFSIVGGEVVLGDKVRLESHV
                     VVDGKTSIGEETRVFPFVSIGLAPQDLKYAGEATQTRIGKKNHIREFVTIHRGTAGGG
                     GLTEIGDNNLLMAQAHVAHDCRIGSEVIMANAATLAGHVTIEDRANVGAYSGVHQFCR
                     IGREAFVGGYSVVVKDALPFAISQGNHAKCYGLNRVGMRRRGYSRETIEKLHHAYHLL
                     LSSKLNTTQAIEKIKEEIRDCAEVDLLVDFIQTSSRGVVK"
     gene            5234..>5498
                     /locus_tag="VGB68_15835"
     CDS             5234..>5498
                     /locus_tag="VGB68_15835"
                     /inference="COORDINATES: protein motif:HMM:NF037151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050763.1"
                     /translation="MKYGLIAGNGKFPFLVVEGARKQGVALAVVAIKEETDKRIEEIA
                     DALIWVGIGQLGKMISFFKKEGVRQAIMAGQVKHVQIFSGALPD"
BASE COUNT         1318 a         1441 c         1427 g         1312 t
ORIGIN      
        1 aataataaaa gggaaccggc tttatgcaca ttcttcgcgc tttcggactt gttttactga
       61 gctttgtcct ctccgccgtg cccgtcgcgg cggaaaccga tgacaaggct aacgaaaatc
      121 cgaaaaccgt aagcaactcg aatcaaccga aaccgcagca gcttgtcgaa gaggttgata
      181 ttcagggaaa ccgtcgtctg cgtgacgaag accttctcta ttacatcaaa acacgcgctg
      241 gcgacgtttt taacccgcag cagctggaac gcgatttgcg cgaactgctt tcgctcaatt
      301 tttttgacaa aaccgccact cgcgttttga ccgaagaagg cgtgcgcggc ggcgtcaagg
      361 tgatttttga ggttcaggag ctgccgatta tccgcgacct gcggtttgaa ggcaacgaag
      421 ccgttcccga atcggaaatc ctcaaacagt ttcgcgaaca gcgggtcggc atctcgaaag
      481 aagccgtttt cgacccggtg aaggtcaacg cggcaaaacg cgttatccgc gagctgctcg
      541 cttcgaaagg ttttccgaac gcgacgatta ccgaaaaaat cgaaaccgtt tccgccacgt
      601 cggtcgccgt tactttcgag gtcgagcagg gcagacgctc gcgtattgtc gatattcagt
      661 ttgaaggcaa cgaggttttc aaggacggcg aattgcgcaa cgcgcttcaa ctggtcagag
      721 aaaccggact tatatcgcgt tttcagggac aggacattct cgatttgcgg aaactgcaat
      781 acgatttaca gaaaaacgtc cgcgattata tgttctcgaa aggatatttt caggcgcgca
      841 tcggcgagcc gcaggtcgag ggcttgggcg tcaggcgcac cgattttatt cctttgataa
      901 cgctgccgct gccgcttatc tcttccaaag acgatacgct ccgcattatc gttcccgtga
      961 cggaaggcaa gctttaccgc gtcggcgata ttaaggtcga aggcaattcg attttttcgg
     1021 aacagcagat tctgaacggt ctcggcttga gaaaaggcga ggtcgccgac ggtcagcgcc
     1081 tgcgcaaagc attgttcgaa gatttgaaaa aagcttacgg ctcgcagggc tttgtgcttt
     1141 acgacgccga gctcgacccg cagtttaaag acaatccggc gaatccgaac gaaggaattg
     1201 tcgacattaa catcgcaatt gacgaaggca aacagtttcg cctgcgccgg ctggaatttg
     1261 cgggcaacac gtttacgcgc gaccgcgttc tgcgccgcga agttttgatt aacgaaggcg
     1321 acatttataa ccagctggcg ctggagacct ccatcattcg tcttaatcaa acgcagtatt
     1381 tcgacccgct tgacaaggat aaggacgtcg aaatccgcac cgacgaagaa accggcgacg
     1441 tcgatttaat cgtcaacgtc aaggaaaaag gcagacagca gatttctttt aacggcggca
     1501 tttccggcat cggcggctcg ttcttcgggc ttgaatattc gaccaacaac cttttcgggc
     1561 gcggcgaagt tttcgcgttc aacgccggtt tcggcaatcg ccagaccact ttgcagcttt
     1621 cctttaccga gccgtatttc cgcgaccgac cgatttccat cggcttttcg ctgttcgctt
     1681 cgcgctacaa gtttttcggc gaaggaacgt ttctgtcgca gaatcaggat ttgttttttg
     1741 atgcgcagaa ccctttcgga acggttctga cggacgaaga taatcttttt acgcagacga
     1801 cctacggcgc gtcggttttc gccaccgcgc cgctttccga atttttaccg aagcgtagat
     1861 tttcgcaatt ttcgcgcgtc ggtttaacgt atcaattttc ggcgaccagc attaaagatc
     1921 cgccggtcaa cgaggacggc aacgccgcga cggaaattcc ggtcgtcttt cgccagccga
     1981 atatcacgac cagccgcatc acgccgagct ttgtctacga ttcgcgccag ccgtcggcaa
     2041 acggaatcga cactttgcgc ggaacgcagc tcgccgtcag cgtaggcttt gccggattgg
     2101 gcggcgacgt acgcacgtat cagccgaatc taacttttac gcattttgtt cccgtccggc
     2161 gtaaacgctc gccgaacgcc gaagttttcg gcttccgcat tcaagccggc accatcggcg
     2221 cgtggtcgat aagcaatgcc gtcaacagcg ccaattcgct ggctttcgtc aacggcgtcc
     2281 cgtttaccga gcgttatttc ctcggcagcg aattcgattt gcgcggttat aatgttcgcg
     2341 ccatcggtcc gattgcgccg gtcgacactt ttatcacctc tcaaaacgtg gtggtggcaa
     2401 cgaatccttc gggcacgccg acgccgatta ccgggctgcc gcccgaacgc gtggcggaaa
     2461 tcgcggcgct cggaactttt accggctcgg gcggcgctaa tcccgggctt atcagccaga
     2521 gctttaccta tatcggcggc gatacgcaat tgctgggaaa ttttgaatat cgcgttccga
     2581 ttttcggtcc catcagcctg gcggcgtttg ccgacatcgg ctcggttttc aatctgcgca
     2641 aaggcaagac gcaggctatc aacagcgcct ttctgcaaga ccagccgttt ctgggagctt
     2701 ttaattcttt gaatatcgtt ttcctgcgca acaacacgca gtttcagccg tcgttttcgg
     2761 gcgggctgct gctgcgcgac ggcagaccgc tgaccagaag cgaatttcaa caaatttatt
     2821 gcggaaactc gccgacgacc tgcccgctga ccgttcccgc cggattgcag gaagtatttc
     2881 tgcgcggcga tgcgcaaacg aatcaactcg tccgggtcga cgagagccgc tttgccaaat
     2941 taggcgattt tcgttcgagc gtcggtctgg aactccgcgt tcaggttccg gtcgtcaacg
     3001 ttccgtttcg ccttatttat tatttcaatc ctaacgcgaa gataggattt actgaagagt
     3061 tgccgaactt ctttctgccc ggcaaacgaa acgggcttcg attctcggtc ggaagaactt
     3121 tttaaaattt tttggaaaac acaagcaaca gttttggaaa aatccgtttc caatggtaaa
     3181 gtcaaaaatt tgaccagcct tttctaccag atttaaggag tattataaaa aatcatgaaa
     3241 acattccgtt caattgccgt cagcttattc tttgcggcac tttttaccgt ctcggctttt
     3301 gcccagacaa cccctacgca gccggcggca ggcagaattg ttacgattaa cactgctatg
     3361 ttcgaggatt cgaaggcggg aattaccaaa tacgttaatg cgatgactgc gctcaataat
     3421 gaattcgagc ctgcttacaa agaattgcag acgatgaaca ccagactgca aactctgcaa
     3481 aaacagtata acgatttata taccgaagcg cagaaaccgg gcagccccat caaacaggaa
     3541 gcccttcagg ctaaacttga cgagattcaa aatcttcagg tcgaattcaa gcgcagacag
     3601 gaagacgcga aaatcaagtt tgagcgccgt caggcagtcg ttatgggtcc ggtgatgcag
     3661 gacattatga aagccatcag cgaatttacc aagcaaaagg gctacggctt ggttctggac
     3721 gctgccaagc tcgacaatgc cggaatcatt ctcggctggg acgaagccaa agtcgacgta
     3781 acaaaagaat ttgttgtttt ctacaacgcg cgcccggcga cgactgccgt caccactccc
     3841 aaaccgtaac ttgctttagt tcagaaatca gaagtcagaa gtcggctctc gcggctggct
     3901 tctgatttct gtattttgta tcctaatttt cttatgaccg ttcttgattc gcgggcaatt
     3961 caggaaatcc tgccgcatcg ttatccgttt ctgctggtcg ataaaatcat cgaactcgtt
     4021 ccgcgcgagc gcatcgtcgg gattaaacag gtaaccatca acgaatattt ttttcgcggg
     4081 catttccccg aagcgcccgt tatgccgggc gttttgatta tcgaggcgct ggcgcaggtc
     4141 ggcgcgattc tcgctttgcg cgaatttgat gaccgcagta aaaaaatccc gtttttcagc
     4201 ggaatcgaaa acgcgcgttt tcgccgtccg gtcgtgccgg gcgatacgct gacgctcgaa
     4261 gtcgtgccgc tcagaatggg cagcaaagtg cagaaaatgc gaggactcgc gcgcgtcgac
     4321 gggcagctgg cggcggaagc cgagattatg tccgtgattg cagacagagg atagaattac
     4381 gaattacgaa ttgccgatta cgaatcagaa gaaataatct ttcgttcgta gccggtattt
     4441 tataattcgt aattgacttt atgactttta ttcatcagac cgcaatcgtc agcccgcagg
     4501 ctgttatcgg cgacgattgc tatatcgggg cgttttcgat tgtcggcggc gaagtcgttt
     4561 taggcgacaa ggttcgtctg gaatcgcacg tcgtcgtaga cggaaaaact tctatcggcg
     4621 aggagacgcg cgtttttccg tttgtctcga tcgggcttgc gccgcaggat ttaaaatacg
     4681 ctggcgaggc tacgcagacg cgaatcggca aaaaaaatca tatccgggag tttgtcacga
     4741 ttcatcgagg aactgcaggc ggcggcggtc tgaccgaaat cggcgacaat aatctgctga
     4801 tggcgcaggc gcacgtcgcg cacgattgcc gcatcggcag cgaagtgatt atggcaaacg
     4861 ccgccacttt agccggacac gtgacgattg aagaccgcgc caatgtcggc gcctattcgg
     4921 gcgttcatca attctgccgc atcgggcgcg aagccttcgt cggcggttac tcggtggtcg
     4981 tcaaagacgc tctgccgttt gccatctcgc agggaaatca cgccaaatgt tacggtttaa
     5041 accgcgtcgg aatgcgtcgg cgcggatatt ccagggaaac tatcgaaaag cttcaccacg
     5101 cttatcacct gctgctttcc tcaaaactga acacgacgca agccatcgaa aaaattaaag
     5161 aagaaattcg ggattgcgcg gaagtcgatt tactcgtcga ttttatccag acctcgtcac
     5221 gcggagtagt caaatgaaat acggcttgat tgccggaaac ggaaaatttc cttttctcgt
     5281 cgtcgaaggc gcgagaaaac aaggcgtcgc tctggcggtt gtcgccatca aagaggaaac
     5341 cgataaacgc atcgaagaaa tcgccgacgc gcttatctgg gtcggcatcg ggcagctcgg
     5401 caaaatgatt tcctttttta aaaaagaagg cgtccgacaa gcgataatgg ctggacaggt
     5461 caaacacgtg cagatttttt ccggcgctct gcccgatt
//
LOCUS       DASWNE010000811        13818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_97350, whole genome shotgun sequence.
ACCESSION   DASWNE010000811 DASWNE010000000
VERSION     DASWNE010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13818
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97350"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..594
                     /locus_tag="VGB68_15840"
     CDS             94..594
                     /locus_tag="VGB68_15840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050764.1"
                     /translation="MDAENPIIEKDVSELTEAEILERIIELGFDGDRHLYEKFCEKLK
                     AGLPPGTGVALRGSVVTNERYEDGRPFDAKGERTSDLDVTLIGDEARAMWTDDAYYIP
                     ALHTKPLGDKDPLIAPALNPLRVELQEMVGRPVNIQATANFIMFVRDVLLGQPYYMIV
                     EQEETA"
     gene            591..1340
                     /locus_tag="VGB68_15845"
     CDS             591..1340
                     /locus_tag="VGB68_15845"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY0050765.1"
                     /translation="MKLKILSYNIRFGGRERQAQIAEVIKAIAPDIVVFQEASDPRVL
                     EYLSDCCRMPFYAAKAKSSVGYVSRLKIAQHQWHHPAGAHHSFLEIQLAETEMRIFGL
                     HLKARFSKWSEQQRHAEIRALLESIKQHQHGFHVIVGDFNTLAPNEVFNLPLMPLWIR
                     ALIWISGRDIRRDTIQEMLNAGYLDGFRNLYPGDKGYTFPVWQPHVRLDYMFVPARYS
                     ELLKSCQVIKEPPVAAQASDHFPLLCEFEII"
     gene            1703..3982
                     /locus_tag="VGB68_15850"
     CDS             1703..3982
                     /locus_tag="VGB68_15850"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0050766.1"
                     /translation="MTLEAKSANQPPEESIPKSAGKNRDYYRRQLEAIAGNATLALFI
                     MDEQQHCVFMNPAAEQLTGYSFAELEGHPLHNYIHHTRPDGRPYPLEECPIDRVFPQD
                     NHEHGEEIFVHKNGSFYPVSFTASPIREGGKTVGTIIEVRNITREKEAERERERGAQE
                     KEKLLIAEKEAREESETLRRIGQIISAELNLQKIVQAVTDAATELTGAQFGAFFYNVL
                     DEEGASYMLYTLSGVAPEKFSNFPMPRATDMFGPTFRGEGTVRIADVHEDARFGKNEP
                     YHGMPKGHLPVTSYLAVSVTSRSGEVLGGLFFGHEKRGVFGERDERIVEGLAAQAAVA
                     MDNARLFEEAQRERAKAERIAADNARLLEEAKEANRLKDEFLATVSHELRTPLTSIIG
                     WVRMLRAGVLDEPQMTRALETIDRNTRAQTQIIEDLLDISRIISGKLRLDIRIIELAG
                     IIEAAIESARPAAEAKNIRLHMLLDRKASPVAGDADRLQQVVWNLVSNAVKFTPKGGR
                     IQVCLERINSHVEISVSDTGQGIEPEFLPHVFERFRQGDSSIARRFGGLGLGLAIVRQ
                     LVEMHGGTVAATSPGENQGATFTVMLPVAIVHNQSFEDSSFKDSEKTFHSPDTDAKIS
                     FRCPPHLEGLRVLIVDDEMDAREMLTFVLNQCQAQVRSAGSASEALAMIDDFNPHILV
                     SDVGMPEEDGYVLIRKVRAREKAANQKPIPAIALTAYTRIEDRMQALSAGFQTHVPKP
                     VEPAELAAVIASLSQWETR"
     gene            complement(4427..6079)
                     /locus_tag="VGB68_15855"
     CDS             complement(4427..6079)
                     /locus_tag="VGB68_15855"
                     /inference="COORDINATES: protein motif:HMM:NF014869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HEY0050767.1"
                     /translation="MKKILGIMGLLALAVSVFISIPYKTGASKNIFGKGTPDEIEKAR
                     EKSLSVIKDKAAQRGIGNDNDFKVKKVEIDKLNMAHTRFQQTLNDIPVWESEAIVHLN
                     ADGELRSVTDNLKGAPAVSTQPNFSAEQALSFAKSFAQISNTIRSKGLTEEPKIDLWI
                     YRGETRDHLVYRVEMPNVENSDNPSVPVVFVDAQTGEKVLEYNNLQTGSGTSLYSGTQ
                     TINTSSAGSTFYMEDLTRRMGTFNMNSTGNTMFGTGGKMARFTGTDDIWNATLEKAGV
                     DAHYGAAKTFDYFLNVHGRNGIDGAGGPRTTAAGANGSISLITSRVHFGKSYNNAFWH
                     NNQMTYGDGDGSQFSPMVTLDIAGHEMTHGVTQYSANLTYSNESGALNESMSDVFGVL
                     VELYADGGVITGNTWKIGEDAYTPGTAGDALRYMDNPHLAGNYGYTTNDDPDHYSERY
                     TGTGDSGGVHINSGIANHSFYLAANGGTHHLSGVNVTGMGTTDAGKIWYRALTVYMTS
                     GTNFAGARTAMQNAATDLFGSTSAQYNSVATAWCAVGVGTCP"
     gene            complement(6941..7303)
                     /locus_tag="VGB68_15860"
     CDS             complement(6941..7303)
                     /locus_tag="VGB68_15860"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0050768.1"
                     /translation="MPKTILVAEDYADARMLMKIMLEGFGYQVVEAADGLEAVEKAKQ
                     FHPDLILMDLAMPVMDGLTATSIISNLDGFDETPIIALTAYSSSYFHKSLQAGCAEMI
                     DKPLDYRKLEPLLEKYLV"
     gene            complement(7890..8435)
                     /locus_tag="VGB68_15865"
     CDS             complement(7890..8435)
                     /locus_tag="VGB68_15865"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY0050769.1"
                     /translation="MIYNSIAEIFKDIDETRAKLIERVSNLPEAEQNFRPSETAWTVK
                     ELTEHLAKTEASIIPLVFRLLKNAEAEGAKSDGTINPPMSLVEQKEKSRTAKFQAPDM
                     IRPEGTTSIPESLAKLEESRETINGIRPRLEAVDLSNTAFPHPAFGKLNLYQWLAFIG
                     LHEGRHVEQIEKILSQARESN"
     gene            complement(8456..9739)
                     /locus_tag="VGB68_15870"
     CDS             complement(8456..9739)
                     /locus_tag="VGB68_15870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050770.1"
                     /translation="MRNFSKILFVVSLALLAACQRNIFQSPTVAPATLRDVPALKLNF
                     RFEPDVPPPSATSQTQASEERVQSVQTDFDTNRQQEVLDKTLVSPDKNRVLAVYHKTE
                     DLQAEFRLDMYSADGRLLRKITPNGMAVHFPDTIVWSPDSTNVAFVGMIRVGNQASAS
                     PGGAPTPAVAEPTPDADANTNVDAETDANANANVAVPAVTPEPPASVLTFRTEQIYLC
                     NADGGDLKPITQNEGLIYFYFAWSPDSAALATLAATIREWQFGQIQADQRMEMFTPSG
                     RPRLVEKTGRIRLLDDNLTKVQPVWSPDSAKVAVAFEKDVRIYDAIGDAPTQAAIPLK
                     NPLLLSSQKFDNELRMKEQGGNVQATNANAEINTLPDENMLVSFNPITELEWTEPAML
                     YLQTGYIREMKNTADSARSYLRWHRLVFSPQAIAL"
     gene            complement(9830..11695)
                     /locus_tag="VGB68_15875"
     CDS             complement(9830..11695)
                     /locus_tag="VGB68_15875"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY0050771.1"
                     /translation="MKLRTDGKIILVLVCAIIFSMNQETSAQRKKPKPKKTTITVTET
                     VPVLPEISYTVSMSKPWTHLLEVEMRLKAPTLAEKTEILMPVWTPGSYLVREYARHVQ
                     DFSARDAAGGVRAWQKTNKNTWQIDSKGAGELLVSYKVYANELTVRTSELNDEHAFFT
                     NAALLMYPKNALAAPSTVKVIPFGNWKIATGLPKVAGQENTFRAENFDVLYDSPFEVA
                     DFAETTFEVRGKSHRWVVTGEGNYDLQKIARDSAKIVEEGAKIFGELPYNDYLFILNL
                     RGGGGLEHLNSTALQWNKFGFKPESRYTDFLTLVAHEHFHAWNVKRIRPDALGPFDYN
                     NENYTRLLWVAEGGTAYYESLLVQRAGLLSDKDFLSGKAAQIQSLQNRPGRFQTSVEE
                     ASFDAWIKYYRQDENAVNNQISYYDKGEIVNFLLDIEIRAASGGAKSLDDVMRHLYNE
                     FYKKNKNYTPEDYQKISEMMAGKSLNDFFSKYVRGREEIDYNSILSGVGLQITTSGRP
                     TAYLGANLSQQGDRLTVTSVPSDTPAYEQGLNTSDQILAVDGYRASQQFLQSYLGEKK
                     VGDKIRLTVFRFDALREIEITLGATKPQNYKIVAVENPTEPQRALYKAYLGTDLK"
     gene            11793..13061
                     /locus_tag="VGB68_15880"
     CDS             11793..13061
                     /locus_tag="VGB68_15880"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0050772.1"
                     /translation="MRQDNFAEKNPQTESEKFFTKPLIIIFVTVFIDLLGFGIVIPAL
                     PYYVESEAFRATPFDIGLLFASYSLMQFIFSPILGSLSDKYGRRPILFFSLLGSALGY
                     LFIGYALALWMVFAGRIIAGITGGNLSTAQAYIADVTTRENRAKGMGFFGATFGLGFI
                     FGPAIGGILSRYGIEVPFLFAAVLSFANAILLYFILPETVKKGSFEEAGKRKNRLAEL
                     FESLRDARFGTLTAIYFFLVTSFSIMTYAFVLYTIFRFGYSAEQNGYIFAFIGFLAVI
                     FQGGFFNRLVHKFGENSLTVVGCLMMTAAFFTIPYIAPDYGGLIGLLAVVAVMAIGNA
                     IAAPALSSLASKISSEKEQGRALGVMQSGASLARAIGPAVGGILLNNALNQVDNTTIQ
                     RTFWTAAAIMIVAVLIAVYFARMEQGQAIA"
     gene            complement(13147..13581)
                     /locus_tag="VGB68_15885"
     CDS             complement(13147..13581)
                     /locus_tag="VGB68_15885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HEY0050773.1"
                     /translation="MRKRFVIHEHHATRLHFDLRLEIGGVLKSWAVPKGVSMNPADKR
                     LAVAVPDHSIGYIGFEGRLAEGTYGAGEVRIWDKGEFETEGEAAEQLRKGKLIFTFYG
                     GKLKGEFALLKMWNQEKNWLIIKSRDAFADKDWKLETVLKKR"
BASE COUNT         3218 a         3513 c         3511 g         3576 t
ORIGIN      
        1 agacgcaaag tgcgcaaagc gtcaaaaatt aaaccggagc tttttcacac aaattatgag
       61 aggctcccaa aatattttaa gcaacaacga attatggatg ctgaaaaccc aatcattgaa
      121 aaagacgttt ccgaattaac cgaagcggaa attttagagc ggattatcga gctcggtttt
      181 gacggcgacc gccatcttta cgaaaaattt tgcgagaaac tgaaagccgg tttgccgccg
      241 gggacgggcg tcgcgctgcg cggcagcgtc gtcacgaatg aacgctacga ggacgggcga
      301 ccgtttgacg cgaaaggaga gcggacgagc gatttggacg tgactttaat cggcgacgag
      361 gctcgcgcga tgtggacgga cgacgcctat tacattcccg cgctgcatac gaaaccgctc
      421 ggcgataaag acccgctgat tgcgcccgcg ctgaatcctt tgcgcgtcga gcttcaggaa
      481 atggtcgggc gtccggtaaa catccaggcg acggcgaatt ttataatgtt tgtccgcgac
      541 gtccttctcg gtcagcctta ctatatgatt gtcgagcagg aagaaacggc gtgaagctaa
      601 aaattttaag ctacaacatt cgtttcggcg ggcgcgagcg acaggctcag attgccgaag
      661 tgataaaggc aatcgcgccg gacatcgtcg tttttcagga agcctcagac ccgcgcgtgc
      721 tcgaatatct gagcgattgc tgccggatgc cgttttatgc ggcaaaggcg aaaagctcgg
      781 tcggatacgt cagccggctg aaaattgccc agcatcaatg gcatcatccg gcgggcgctc
      841 atcattcgtt tctggaaata cagctggcgg aaacggaaat gcggattttc ggcttgcatc
      901 tgaaagcgcg tttttccaaa tggagcgagc agcagcgtca cgcggaaatc cgcgctttgc
      961 tcgaaagcat caaacagcat caacacggct ttcacgtcat cgtcggcgat ttcaacacgc
     1021 ttgcgccgaa cgaagttttc aacctgcctc tgatgccgct ctggattcgc gccctgattt
     1081 ggatttcggg acgcgacatc cgccgcgaca cgattcagga gatgctcaac gccggatacc
     1141 tggacggctt tcgcaatctt tacccgggcg ataaaggcta cacgtttccc gtctggcagc
     1201 cgcacgtccg gctcgattat atgtttgttc cggcgcggta ttccgaactt ttaaaatcct
     1261 gtcaggttat taaagaaccg ccggttgcgg cgcaggcttc agaccacttt ccgcttttgt
     1321 gcgagtttga aatcatctga aaaaaaattt taaagtaaag gcggcaactg ttcagagaga
     1381 cgataatctg ccacgataaa aggttttttg gagatacaac aggaacatta attatctttt
     1441 cttccttatg gctgcgagtc gcgaaaccgg ctcgtccttt gttaattttc tttgtttccc
     1501 gcgcgtattc gtcgtgtatt ttccttcctc ataaaatttg aaacattgcc gaattttgaa
     1561 attaaaagcg ggcgggcagg cggctttttc aatttttaaa atgcggcgcg ccaagaaaca
     1621 tcttgtgaag aagtttccgt ccgtttataa tttaatttca cgagcaagga ttattcgtct
     1681 ttaaaagcga gtagggaggg ttatgacgct agaggctaaa tctgcaaacc agccgccgga
     1741 agaatcgatt ccaaaatcag ccgggaaaaa tcgagattat tatcgccgcc agttagaggc
     1801 gattgccggt aatgccacgc tcgcgctgtt tattatggac gagcagcagc actgcgtttt
     1861 tatgaatccg gcagcggagc aattgacggg ttactctttc gccgagctgg aaggtcaccc
     1921 gctgcataat tacatccacc acacgcgccc cgacggtcgc ccctacccgc tcgaagaatg
     1981 tccgattgac cgcgtctttc cgcaggataa tcacgaacac ggcgaagaaa tcttcgttca
     2041 caaaaacggc tctttttacc cggtcagctt taccgccagc ccgattcgcg aaggcggtaa
     2101 aaccgtcggc acgatcatcg aggttcgcaa catcacacgc gaaaaagaag cggagcgcga
     2161 gcgcgagcgc ggcgcgcagg aaaaagaaaa acttttaatc gccgaaaaag aagcgcgcga
     2221 agaaagcgaa acgctccggc gcatcgggca gataatttcc gccgaattga acctgcaaaa
     2281 aatcgtgcaa gccgtcaccg acgcggcgac cgaattgacc ggcgcgcagt tcggcgcatt
     2341 tttttataac gttttggacg aagaaggcgc cagttatatg ctttacacgc tttccggcgt
     2401 cgcgcccgaa aaattctcga atttcccgat gccccgcgcc accgatatgt tcggtccgac
     2461 ttttcgcggc gaaggaaccg tgcgcatcgc cgacgtccac gaagacgcgc gtttcggcaa
     2521 aaacgagcct tatcacggga tgccgaaagg tcacttgccc gtcaccagtt atttagccgt
     2581 ttcggtcacg tcgcgctcgg gcgaagtttt gggcgggctg tttttcggac acgaaaagag
     2641 aggagttttc ggcgaacgcg acgaaagaat cgtcgaggga ctggcggcgc aggcggcggt
     2701 ggcgatggat aacgcgcgtc tttttgaaga agcgcagcgc gagcgcgcca aagccgaaag
     2761 aattgccgca gacaatgcgc gcctgctcga agaagccaaa gaagccaacc gcctgaaaga
     2821 cgaatttctg gcgaccgtat cgcacgagct gcggacgcct ctgacttcga taatcggctg
     2881 ggtcagaatg ctgcgggcgg gagtgctcga cgagccgcag atgacgcgcg cgctggagac
     2941 gattgaccgc aacacgcgcg cgcagacgca gattatcgaa gacctgctgg atatttcgcg
     3001 aattatttcc ggcaagctgc gcctcgatat tcgtatcatc gagctcgccg gtattatcga
     3061 agcggcaatc gaatcggcgc gtccggcggc agaagccaaa aacatcaggc tgcatatgct
     3121 gctcgaccgg aaagccagcc cggtcgcggg cgatgccgac cgcctgcaac aagtggtctg
     3181 gaatttagtt tccaacgccg tgaaatttac gccgaaaggc gggcgcattc aggtgtgtct
     3241 ggaaagaata aattcgcacg tcgaaatttc cgtcagcgac acgggacaag gcatcgagcc
     3301 cgaatttctg ccgcacgttt tcgagcgttt ccggcagggc gattcttcga ttgcccgccg
     3361 gttcggcggc ttgggattag gcttggcgat tgtgcgtcag ctggtcgaga tgcacggcgg
     3421 aacggtcgcc gcgaccagcc cgggcgaaaa tcagggcgcg acctttacgg tgatgctgcc
     3481 ggtggcgatt gtccataatc aatctttcga ggatagctct ttcaaagata gtgagaaaac
     3541 atttcattcg ccggatacgg atgcgaaaat ctcctttaga tgcccgccgc atttggaggg
     3601 gctgcgcgtt ctgatagtcg acgacgagat ggacgcgcgc gagatgctga cttttgtgct
     3661 gaaccagtgt caggcgcagg ttcggtcggc tgggtcggca tccgaagcgc tggcaatgat
     3721 agacgacttt aacccgcaca tactggtcag cgacgtcgga atgccggaag aagacggtta
     3781 tgtcttaatc cgaaaggtgc gcgccaggga aaaagccgcc aaccagaagc cgattccggc
     3841 tatcgccctg acggcttaca ccagaatcga agaccgtatg caggcgctct cagccggctt
     3901 tcaaacgcac gtgccgaaac cggtcgaacc ggctgaactg gcggcggtga ttgccagcct
     3961 gtcgcagtgg gaaactcgct aagtgttttt atgaacaatt taccggcaga aaaataatat
     4021 gcattttttt tggaactttc cgttaatctg ggcgtgttta tatttgaata tacggaattt
     4081 tctcctccga tgtttaatac agaatagctt aaagcccgtc tgaaaaggcg ggctcttttt
     4141 tcttgaaaat ccgccgcgtc gatttttcat tcctcgaaag cgaccggaca tttttaaaga
     4201 ttaccggatt tttattttgt gcgattgtgg aaaaaaatcc gtctaaaaaa atccgtctga
     4261 atagaagaac tttcatcgcc ggattttaat ttattcgaga aatttccgat ttttaattgg
     4321 aaagattagt tttaaaatcg gttcagggcg gaacaccgtc ttcaaatcta ctgcaataag
     4381 aagtccagtt atgccgccct gagtttcagt cgaatccgtt gtttagttac gggcaagttc
     4441 cgacgccgac tgcgcaccag gctgtcgcaa ccgaattgta ttgcgcgctg gtcgagccga
     4501 aaaggtcggt cgccgcattc tgcatcgccg ttctcgcgcc cgcgaaatta gtgcccgatg
     4561 tcatatacac ggtcaacgcg cgataccaga tttttcctgc atcggtcgtt cccattcccg
     4621 tgacgttgac gccgctcagg tgatgcgttc cgccgtttgc cgccagataa aacgagtggt
     4681 tggcgatgcc ggaattgatg tgaacgccgc cgctgtcgcc ggttcccgta tagcgttcgg
     4741 aataatggtc ggggtcgtcg ttggtcgtat aaccgtagtt tccggctaag tgcgggttgt
     4801 ccatatagcg aagcgcgtcg ccggctgttc cgggcgtgta agcgtcttcg ccgattttcc
     4861 aggtgttgcc ggttatcact ccgccgtcgg catacaattc gactaaaaca ccgaaaacgt
     4921 ctgacatcga ttcgttcagc gcgccggatt cgtttgaata agtcagatta gccgaatact
     4981 gcgtcacgcc gtgcgtcatt tcgtgtccgg cgatatccag cgtgaccatc ggcgaaaact
     5041 gcgagccgtc gccgtcgcca taagtcattt gattgttatg ccaaaaagcg ttgttgtagc
     5101 ttttgccgaa atgcactctt gaagtaatta agctgattga gccgttcgcg cccgcagcag
     5161 tcgttctggg accgcctgcg ccgtcgatgc cgttgcgacc gtgaacgttc aggaaataat
     5221 cgaaggtttt cgccgcgccg taatgcgcgt caactccggc tttttccagc gtcgcgttcc
     5281 aaatatcgtc cgtgccggta aaacgcgcca tctttccgcc cgtgccgaac attgtattgc
     5341 cggtgctgtt catattgaaa gtgcccattc tgcgggtcaa atcttccata tagaaggtcg
     5401 agccggcgga actggtatta atcgtctggg tgccgctgta aagcgaagtc cccgaacctg
     5461 tctgcagatt gttgtattcg agcacttttt cgcccgtttg cgcgtcgacg aaaacgaccg
     5521 gaacggacgg gttgtcgctg ttctccacat tcggcatctc gacgcggtaa actaaatggt
     5581 cgcgcgtttc gccgcgataa atccagaggt cgattttcgg ctcttcggtt aacccttttg
     5641 aacggatagt gttggagatt tgagcaaacg atttggcaaa gctcaatgcc tgttccgccg
     5701 aaaaattcgg ctgcgtgctg accgccggcg ctccttttaa attgtccgtt acgctgcgca
     5761 gttcgccgtc ggcattaaga tggacaatcg cctcgctttc ccagaccgga atatcgttca
     5821 gggtttgctg aaaacgagta tgagccatat tcagcttgtc gatttcgact tttttcacct
     5881 taaagtcgtt atcattgccg atgccgcgct gcgcggcttt atcttttata acgcttaaac
     5941 ttttttctct ggctttttcg atttcgtcgg gtgtgccttt accgaaaatg tttttgcttg
     6001 cgccggtttt gtaagggatt gagataaaaa ccgaaaccgc taaagcaagc agccccataa
     6061 tacctagaat ttttttcacg cttatttact ccttagagaa tttaattttt atttgccgat
     6121 aatatatcaa gcctgtatga atgaaatgaa gttttcaggc tgaaaagctt ctaagtataa
     6181 aatttaaact ggttgatttg tttaaagtaa agcatttgaa aatgggaaga ttttcaaagc
     6241 tgtcggtata aggttatctt taagagagta acatttacaa ttaaagtgtc aagaaaaaat
     6301 cgaccgggac ggcttcagcg aaaacatttt gataaaaacg ggtgattttt aagccgtgtt
     6361 ttcgagagat gctttcaatg ccgcgctcac ctgaagaatg attcttcatc gccgaacgct
     6421 tttgattaca acatagcgga cagcttattt tgtaagatta acagtcgaat gccgattgac
     6481 ttgtgattgc ggttaattca aaatagtttc ggtcaactac acagaatagt ttacgggctt
     6541 cgcgttgact gcccgctcgt tcactcatcg aacaaggcgc ttcacaaatt aggggacgcc
     6601 ttttttattt tcgcttccgg ctaaaaccgg ctcgcaaatc agtctgacaa gtaaaaaaat
     6661 cgctcaagaa ccggcgttgt cgccggcgct cgaagcggct gagtaatatt ccaaacgagt
     6721 taaataaaat gagttaaata aaatgaataa tcttcgcgct ccggctccaa gactattccg
     6781 agccggaaca ttattcatcg ttcaaatttt ttttgagcca aagccgtaaa tgtattgaag
     6841 cgcgtgactt tcgacaacat ttttttgagc cgccggaaaa taattgcttt ccggcggctc
     6901 aagattagaa caccgcattc tgcccgggtt tacgccaaca tcagacgaga tatttttcca
     6961 gcagaggctc gagttttcta tagtcgagag gcttgtcaat catctcggca cagcccgctt
     7021 gcagcgactt gtgaaagtat gaactgctgt aggcggtcag agctataatc ggagtttcgt
     7081 cgaaaccgtc taaattactg attatgctcg tcgcggtcaa gccgtccatc acgggcatcg
     7141 ccaaatccat caaaatcagg tcgggatgaa actgtttggc tttttcgact gcttccaagc
     7201 cgtctgccgc ttctactact tgataaccga atccttcgag cataattttc atcagcattc
     7261 gagcgtcggc gtaatcttcg gcgactaaaa tggttttggg catagtgtgg gtaccttttt
     7321 gttaaaacct atttaacatc atatagaaat aaagcattta atacaatatt tttattttgg
     7381 tcgcgatata aaaacctctt ccaccgtcag ggcgaccgct tattacatag ctttattaat
     7441 ttatgtcgca ggcgggaaaa tcaattcgag gcaataaaaa tcggcaagcc ggcgaattgc
     7501 cgaacttgcc gatttagctg taaatgccct cgataggatt tgaacctata accaacggat
     7561 tatgcctgcc agctacggct ttcgccgccc tttcggtttg tggtctggac tttctcttct
     7621 ctttacgagt ccgccgttaa gtctctacac cttccggttt ttcgaccggc ttggctcggg
     7681 attaccgtgt gtctttcgac atttaggctt ccccgaattt gacggattct actgaaatgc
     7741 tgtttataag acaacaaatc ggcaacccat tttttgaaac cgtatttttc aacttaggtt
     7801 tccgtcgttt gagtccgctg ctctaaccgt tgagctacaa gggcaaacag ggaatagttt
     7861 agtaaaaagt tttgcggcta gcaaaccgtt taatttgatt cgcgcgcctg cgacagaatt
     7921 ttctcgattt gttcgacgtg tcgtccttcg tgcagaccga taaaagccag ccattgataa
     7981 agatttaatt tgccgaaagc cggatgcgga aaagccgtgt ttgataaatc gacggcttcg
     8041 agccgcgggc ggatgccgtt aatagtttcg cgcgattcct caagcttcgc cagcgattcg
     8101 gggatgctcg tcgttccttc cgggcgaatc atatcgggcg cttgaaactt cgccgttctt
     8161 gacttttcct tctgctcgac cagcgacatc ggcggattta tcgtgccgtc ggactttgcg
     8221 ccttcggctt cggcattttt cagcagccga aaaacgagcg ggataatgct ggcttcggtt
     8281 ttcgccagat gctcggtcag ctctttcacc gtccaggcgg tttcggaagg tcgaaaattt
     8341 tgctcggctt ccggtagatt cgagaccctt tcaattagtt tcgctctggt ttcgtcgatg
     8401 tctttaaaaa tttcggcgat gctgttgtaa atcattcttc ggtttccttt tcttcttata
     8461 aagcgatggc ttgcgggctg aaaaccagac ggtgccagcg cagatagctg cgcgcgctgt
     8521 ccgccgtgtt tttcatctcg cggatatagc cggtctgcaa ataaagcatc gccggttcgg
     8581 tccattccag ctcggtaatc gggttaaacg agacgagcat attttcgtcc ggcaaagtgt
     8641 tgatttccgc gttcgcgttg gtcgcctgca cgtttccgcc ctgttctttc atacgcagct
     8701 cgttatcgaa tttttgcgag gaaagcagca gaggattttt cagcggaatc gcggcttgcg
     8761 tcggcgcgtc gccgatggca tcgtaaatcc gcacgtcttt ctcaaaggcg accgccactt
     8821 ttgccgaatc gggcgaccag acgggctgca ctttcgtcag attatcatcc agaagccgga
     8881 ttctgccggt tttttccacc aatcgcggtc tgcccgacgg agtaaacatt tccattcgct
     8941 ggtcagcctg aatctgtccg aattgccatt cgcgaatcgt cgccgccaaa gtcgccagcg
     9001 cggcgctgtc cggcgaccac gcaaaataaa aataaatcaa gccttcgttt tgcgtaatcg
     9061 gctttaaatc gccgccgtcc gcgttgcaga gataaatctg ctcggtgcga aacgtcaaaa
     9121 ccgacgcggg cggctccggc gtgacagccg gaacggcgac gttcgcattc gcgttggcgt
     9181 ccgtttcggc atcgacattc gtgttcgcat cggcgtccgg cgtcggctcg gcaactgccg
     9241 gcgtcggcgc tccgcccggc gacgcgctcg cctgattgcc gacgcgaatc atcccgacaa
     9301 acgcgacatt ggtcgaatcg ggcgaccaga caatcgtgtc cggaaaatga accgccattc
     9361 cgttcggcgt gatttttcgc aaaagcctgc cgtccgccga atacatatcc agccgaaatt
     9421 ccgcctgtaa atcttccgtt ttgtgataaa cggctaaaac gcgatttttg tccggcgaaa
     9481 cgagcgtttt atccagcact tcctgctgtc gattcgtatc aaaatcggtc tggacggatt
     9541 gaacgcgctc ctcgcttgcc tgcgtctgac ttgtcgcgga cggcggcgga acgtccggct
     9601 cgaatcgaaa attcagcttc aaagccggaa cgtcgcgcaa ggtcgcgggc gcgaccgtcg
     9661 gcgactggaa aatattgcgc tggcaagccg ctaaaagcgc aaggctgacg acgaaaagaa
     9721 tttttgaaaa gtttcgcatt tttcagaata gaattagaga agaaacgcgg aacgatgaat
     9781 gcggaacgcg gaatttgcaa ttcatcgttc atcgttccca gttcatcgtt tatttcaaat
     9841 cagttcccag ataggcttta tacaaagctc tctgcggctc ggtcggattt tcgacggcga
     9901 cgattttata attctgcggt ttggttgcgc cgagcgtgat ttcgatttct cttagcgcgt
     9961 caaagcgaaa aaccgtcagc cggattttat cgccgacttt tttctcgccc agatacgact
    10021 gcaaaaactg ctggctggcg cgatagccgt caaccgcgag gatttggtcg ctcgtattca
    10081 agccctgttc gtaggcgggc gtgtccgacg gaaccgaagt gacggtcagg cggtcgccct
    10141 gctgcgagag atttgcgccg agataggctg tcgggcgtcc gctggtcgtt atctgcaagc
    10201 cgacgccgct taaaatcgag ttgtaatcga tttcttcgcg cccgcgcacg tatttcgaga
    10261 aaaagtcgtt taaggatttc ccagccatca tttccgagat tttctggtaa tcttccggcg
    10321 tgtaattttt atttttcttg taaaattcgt tatacaaatg acgcatcaca tcgtccagcg
    10381 attttgcgcc gccggacgcc gcgcgaatct cgatgtcgag cagaaaattg acgatttcgc
    10441 ctttatcgta atacgaaatc tgattgttga ccgcgttttc gtcctgccgg taatacttaa
    10501 tccacgcatc gaagctggct tcttcgacgc tggtttgaaa gcgtcccggg cgattttgca
    10561 ggctttgaat ctgcgcggct ttcccgctca gaaaatcttt gtcggaaagc aaaccggcgc
    10621 gctgcacgag caggctttcg taataagccg tgccgccttc ggcaacccag agcaggcgcg
    10681 tgtaattttc gttgttgtaa tcaaacggac cgagcgcgtc gggacgaatg cgtttgacgt
    10741 tccacgcgtg aaaatgctcg tgcgcgacga gcgtcagaaa atccgtgtag cgcgattcgg
    10801 gcttaaagcc gaatttattc cattgcagcg ccgtcgaatt aagatgttcg agaccgccgc
    10861 cgccgcgcaa attcaggata aaaagataat cgttatacgg cagctcgccg aaaatcttcg
    10921 cgccttcttc gacgattttc gccgaatcgc gcgcgatttt ctgcaaatcg taattgcctt
    10981 cgcccgtcac tacccagcga tgcgatttgc cgcgcacttc aaaagtcgtt tcggcaaaat
    11041 cggcgacttc aaacggcgaa tcgtaaagaa cgtcgaaatt ttcggcgcga aaagtgtttt
    11101 cctgcccggc gactttcggc aagccggtgg cgattttcca atttccgaac ggtatgactt
    11161 tcacggtcga aggcgcggcg agagcgtttt tcggatacat cagcagcgcg gcattcgtaa
    11221 aaaaagcgtg ctcgtcattt aattcgctcg tgcggacggt caattcattg gcgtaaactt
    11281 tgtagctcac cagcaattcg cccgcccctt tcgaatcaat ctgccacgta tttttatttg
    11341 tcttttgcca cgcgcgaacg ccgccggcgg cgtctctggc ggaaaaatcc tgcacgtgcc
    11401 gcgcgtattc gcgcaccaga tagctgcccg gcgtccagac cggcatcagg atttccgttt
    11461 tttccgccag cgtcggagct ttcagacgca tttcgacctc gagcaggtgc gtccacggct
    11521 tcgacatcga aaccgtgtag ctgatctccg gcaaaaccgg aacggtttcc gttactgtta
    11581 tcgttgtttt tttcggtttg ggcttcttcc tttgcgcgct ggtttcctga ttcatagaaa
    11641 aaataatggc acagactaaa actaaaataa tcttcccatc ggttcgtaat ttcattgctt
    11701 tggcaaactc cgtaatgatg ttatgtttta attttgttat cttactacaa aacaaaaatc
    11761 tgagcagtaa aggctctaaa tttaaaattt taatgcgcca ggataatttt gccgaaaaaa
    11821 acccgcaaac ggaaagcgaa aagtttttta ccaaaccgct tatcattatt ttcgtcacgg
    11881 tttttatcga ccttttaggt ttcggcatcg tcattcccgc tctgccatac tacgtcgaaa
    11941 gcgaagcctt tcgagccacg cctttcgata tcgggctgct tttcgcttcc tacagcctga
    12001 tgcagtttat tttctcgccg attctgggca gcctctcgga taaatacgga cgccgaccga
    12061 ttttgttttt ttccctgctc ggttcggcgc tcggttatct gtttatcggc tacgccttgg
    12121 cgctctggat ggttttcgcc ggtcgcatta tcgcgggcat cacgggcgga aatctctcga
    12181 ccgcgcaagc ctatatcgcc gacgtgacga cgcgcgaaaa ccgggcgaaa ggaatggggt
    12241 ttttcggcgc gacattcggt ctgggcttta tcttcggtcc cgccatcggc ggaattttaa
    12301 gtcgctacgg catagaggtc ccgtttctgt tcgccgccgt tttgtctttc gccaacgcga
    12361 ttctgcttta ttttattctg ccggagaccg tcaaaaaagg ctcgtttgaa gaagcgggga
    12421 aacgcaaaaa tcgtctggcg gagctttttg aatctttgcg cgacgcgcgt ttcggaacgc
    12481 tgacggcgat ttactttttt ctggtcacgt cgttttcgat aatgacctat gccttcgtcc
    12541 tttacacgat ttttcgcttc ggctacagcg ccgagcaaaa cggttatatt tttgctttta
    12601 tcggcttttt ggcggtgata tttcagggcg gattcttcaa tcgcctggtg cataaattcg
    12661 gcgaaaattc tttaaccgtc gtcggttgtt taatgatgac cgcggctttt tttaccattc
    12721 cgtatatcgc gcccgattac ggcggattga tcggcttgct tgcggtcgtc gccgtgatgg
    12781 caatcggaaa cgcgatagcc gcgcccgctt tatcgagcct cgcgtcgaaa atatcgagcg
    12841 aaaaagaaca gggcagagcg ctcggcgtga tgcagtccgg ggcgagcctg gcgcgggcaa
    12901 tcggtcccgc cgtcggcggc attctgctga acaacgcgct caatcaggtt gataatacga
    12961 ccattcaaag aaccttctgg acggcggcgg cgattatgat tgtcgcggtt ttaatcgcgg
    13021 tctattttgc gagaatggaa cagggacagg cgattgcctg agaagaaacg acgaatgatg
    13081 aacgcggaac gatgaatagg aaaggcaaaa cgcggtaact gtaattcatc gttcatcgtt
    13141 ccgcgttcat cgtttcttta aaaccgtttc cagcttccag tctttatcgg caaacgcatc
    13201 gcgcgatttg ataatcagcc agttcttttc ctggttccac attttcagga gcgcaaactc
    13261 gccttttaat tttccgccgt aaaacgtaaa aatcagcttt ccctttcgca attgttccgc
    13321 cgcctcgccc tcggtttcaa attcgccctt atcccaaatg cgcacttcgc ccgcgccgta
    13381 agttccctcg gcgagcctgc cttcgaaacc gatataaccg atggaatggt cagggaccgc
    13441 gacggctaat cttttatccg cgggattcat cgaaacgcct ttcggaaccg cccacgattt
    13501 taaaacgccg ccgatttcca gacgcaaatc gaaatgcagc cgcgtggcgt gatgctcgtg
    13561 aatgacaaag cgctttcgca taattctatt ttgccacaga gaatttcaag gcaaaaggcg
    13621 aaagtaaaaa ggcaaaatta cggactgatt tattttttat ctttcgcctt ttgccttatt
    13681 ccgctctgtg gcaaaataaa aatatgaaaa gagccgtcgg cgtcggcggc atatttttca
    13741 aagcgcgaaa cccgaaagtt ttagcttaat ggtatgaaaa atatcccggc atcgcttgcg
    13801 gcggcaaagc ttacacgg
//
LOCUS       DASWNE010000812         6181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_166641, whole genome shotgun sequence.
ACCESSION   DASWNE010000812 DASWNE010000000
VERSION     DASWNE010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6181
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166641"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VGB68_15890"
     CDS             complement(<1..317)
                     /locus_tag="VGB68_15890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050774.1"
                     /translation="MSDVIRKFISRLNLSFVENRSARRYNLQVPVRITIEPDETNGKL
                     TTRRESLSASGETKDLSKSGIAFIVSSIRLREYYLVGENRTLQAELDLPNGKIRMQIV
                     GVR"
     gene            complement(405..1319)
                     /locus_tag="VGB68_15895"
     CDS             complement(405..1319)
                     /locus_tag="VGB68_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050775.1"
                     /translation="MFNKLSKANFHLKMSLFGACLSIAAVSIGCTASAKNHDETSAVQ
                     TEITEEKNNAAPNAAKNSYVKGRIQVKANSPADAVRTFYKNLRERRFREAMLMTNARP
                     AIEGLTDAEMQDLSADFEPIAKQVPPDIQINGEIVTGNLANVTAKMPNDETGVLEDKV
                     FNLRRENDAWIILTADEKAEALVKKEGKNYFFGIRVEVHHVEAQLMLERITKAQTIYA
                     LRNPNSFADMQTLIDQGLLAGDALGTESTGYRYSISVSPDKKKYFATAEPAVYGKTGK
                     LSFLVEVEGANKQARLKSQDKKGQSLKK"
     gene            complement(1466..3562)
                     /locus_tag="VGB68_15900"
     CDS             complement(1466..3562)
                     /locus_tag="VGB68_15900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY0050776.1"
                     /translation="MLHQNNMRLLFLKIFLCFSGLVFGFSVLTETAFLQNTPPKPQST
                     VKRTATPRPKKKASTNNAEIVMETREVTGIGNTATSDVPTPTPANSTVRGKVFYADTG
                     RAVKRAPIMLMREKGLGGPGTSPTGLTDNDGNFQMKGVQAGIYYAMVNAPGVVSPLAF
                     MDFSKARGGASEAEEFEKAAEGFEKIIVDGITDLDVQIPARRGGAIGGRLMYDNGDPA
                     IGVKVEALRKVNNKFMPVIPNFSAIFSMMTGSGSYQTDDRGIYRFSGLPAGEYIVKVT
                     ESVSHYESGEKRSYNEFETMFGSGNSLLSMFYPDVFEPKAANIINLELGQEIVEINMT
                     IPSRNLYKIEGKIVARKDKTPVKAKITLKREGDEDLFSIFSEISRRRHSSVTDENGNW
                     KFKELPKGNYKLLIEPVQTETDYGEVLDDYYNSNMTTNVQKNSPPKPKFAGKIQEIKL
                     EDKDLSEMVIELGYGATIAGTISVENGEKMPKSVTVAATNADNELSVSDVISNYDGSP
                     ITRTMSSNTNVIRSAGNVMQTTGSADNDFELENVAEGKTDIYIHIADENYYVKSAIAD
                     GVDLLGGKIEIKDGDVIRNVRVVLSKNVGTLKGKVVSENNEPLKNTQFTLAPTDAARR
                     TSATYFRLVKTDANGEFETKLPPWEFAVVFVDSENFPKKAAEIDEWYNKAVKDAQKVT
                     VEAGRTENLTVRKIKK"
     gene            complement(3667..6027)
                     /locus_tag="VGB68_15905"
     CDS             complement(3667..6027)
                     /locus_tag="VGB68_15905"
                     /inference="COORDINATES: protein motif:HMM:NF015148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HEY0050777.1"
                     /translation="MKRNPFLEKFSPYIPAAVTNMPELSIFPLIVGTLLGVVFGASSL
                     YLVLKVGLTVSASIPVAVISITLFRLLSKARITRDATILEHNIVQTAGSAGESIAFGL
                     GVTMPAILILGFDLELSRVMLVAVLGGLLGILMMIPLRRALIKDQHGILKYPEGTACA
                     QVLIAGASEESRAASIESEKVRADESATNGGKVIAIGFGIGFLFNTLMQVFKGWKEVP
                     GKQFDAPFRGGSISLENNPALLGVGYIIGPKIAGIMFAGGALSYLVLIPMIVYFGEFL
                     QTPLAPETTKTIHDLFYIPVADGGGFNFAAGIRNIQSSYVLYIGAGAVAAGGMISLAR
                     SLPTIWHGLKGGLADFRGSTGSSSAAETDNAPRTDRDLSMKWVVIGILALIIVITLAP
                     ALKMNLLGAILIVIFGFLFVTVSSRLTGEIGSSSNPISGMTVATLLLTCLVFLIIGWV
                     EPDPYFVTALSIGGIVCIAASNGGTTSQDLKTGFWVGGTPWKQQIAILIGATASALLL
                     GPILIQLNTSSTVYQKVEPAAFSQQFVVPEQQLSRDGAQLKGEKVADAYFNDQNTYRV
                     WHNTDSANGKPGRYLIDTQGKPVYLVDPGIGGTVTRRADNTEVVKYDPPKATLMSYII
                     KGILSQQLPWGLVLLGVMIAVVLELAGIPSLAFAVGLYLPISVSAPIFVGGIVRWAVD
                     IYLKKKLANRNLTEDEIIAETDKSPGVLLASGYIAGGALAGILLAVATEFLSKRMDAF
                     QTWSQANNPFFAGPNSDWLGLIPFWILAILLYYVGRELLLSGRKPR"
BASE COUNT         1391 a         1652 c         1564 g         1574 t
ORIGIN      
        1 tatctgacgc cgacgatttg cattcgtatt ttgccgttcg gcaaatccag ctcggcttgc
       61 aaagtgcgat tttcgccgac cagataatat tcgcgcaggc gaatcgagga gacgataaag
      121 gcaatgccgg atttgcttaa atcttttgtt tcgccgctgg cggaaaggct ttcccgcctg
      181 gtcgttagct ttccgttcgt ctcgtccggc tcaatcgtaa tcctgacggg cacctgcaaa
      241 ttatagcgcc gcgcacttcg attttcgacg aatgacaggt ttagcctgga aataaatttg
      301 cggattacat ctgacattta actgggcaac cgccttaaac ccgataaccg acgtttgcgg
      361 aagaataacg tcgccggttt atgataaatt tcaaccgctc tgcgttattt tttcagcgac
      421 tgaccttttt tatcttggct tttgagccgc gcttgtttat tcgcgccctc gacctcgacc
      481 agaaatgaaa gctttcccgt tttgccgtag acggctggct ctgccgtcgc aaaatatttc
      541 tttttgtccg gcgaaaccga gatgctgtag cgatagccgg tcgattcggt gcccagcgcg
      601 tcgcccgcca gcaagccctg gtcgattaat gtctgcatat cggcgaagct gttcgggttt
      661 cgcagggcgt aaattgtctg cgctttggtg atgcgctcaa gcatcagctg ggcttcgacg
      721 tgatgaacct cgacgcgaat gccgaaaaag taatttttgc cttctttttt gaccagcgct
      781 tcggcttttt catctgccgt caaaataatc caggcgtcgt tttcgcggcg cagattaaaa
      841 actttgtctt ccagaacgcc ggtctcgtcg ttcggcatct tcgccgtcac gttcgccaga
      901 tttcccgtga cgatttcgcc gttgatttga atatccggcg gaacctgttt ggcaatcggc
      961 tcgaaatcgg cgctcaaatc ctgcatttcc gcgtcggtca agccttcgat tgccggacgc
     1021 gcgttggtca tcagcatcgc ctcgcggaaa cggcgctcgc gcaggttttt ataaaacgtg
     1081 cgcacggcgt cggcgggcga attcgccttg acctggattc tgcctttgac atatgagttt
     1141 ttcgccgcgt tcggcgcggc attgtttttt tcttcggtga tttcggtctg gacggctgaa
     1201 gtttcgtcgt gattttttgc cgatgccgtg cagccgatgc tgacggcggc aatcgaaaga
     1261 caagcgccga aaagagacat tttcagatga aaattcgctt tggaaagttt gttgaacatt
     1321 gattttgact ccgaaaaaac tgacggaaaa ttttgaaatg aatggcgcga aagttttgcg
     1381 ggaacgggcg ttcaggagaa agcaaaacgc gctaccggtg attatagcgc gttccgcatc
     1441 gaaaagtaaa caatgttttg catttttatt tctttatttt tctaaccgtc agattttcgg
     1501 ttcttccggc ttcgacggtg actttttggg cgtctttcac ggctttgtta taccactcgt
     1561 cgatttccgc cgctttttta ggaaagtttt ccgagtcgac gaagacgacg gcaaattccc
     1621 acggcggcag cttcgtttcg aattcgccgt tcgcgtcggt tttgacgagg cggaaatacg
     1681 tcgcgctcgt gcgccgcgct gcgtcggtcg gagccagagt aaattgcgtg tttttgagcg
     1741 gctcgttgtt ttcgctgacg acttttccct tcagcgtgcc gacgtttttg ctgagaacga
     1801 cccgcacgtt tctgataacg tcgccgtcct ttatttcaat tttaccgccg agtaaatcga
     1861 cgccgtcggc aatcgcgctt ttgacgtaat aattttcgtc ggcgatgtga atatagatat
     1921 cggtttttcc ttcggcgacg ttttccaatt cgaagtcgtt gtcggcgctg cctgtcgtct
     1981 gcatgacatt accagccgag cgaattacat tggtgttgct gctcatcgtc cgcgtaatgg
     2041 ggctgccgtc ataatttgaa ataacgtccg acacgctgag ctcgttatcc gcgttggtcg
     2101 cggcgaccgt tacgctcttc ggcattttct cgccgttttc gacgctgatc gtgcccgcga
     2161 tggtcgcgcc gtagccgagc tcgataacca tttccgaaag gtctttatct tcgagcttta
     2221 tctcctgaat cttaccggca aatttcggtt tcggcggcga atttttctgc acgttcgtcg
     2281 tcatattgga attgtaataa tcgtccagaa cttcgccgta gtcggtttcg gtctgaaccg
     2341 gctcaatcag gagcttgtaa tttcctttcg gcaattcctt aaacttccag ttcccgtttt
     2401 cgtcggtcac cgagctatgt ctgcgccggc tgatttcact gaagattgaa aagaggtctt
     2461 catcgccttc acgctttagg gtaatttttg ctttgacggg cgttttatct ttgcgcgcga
     2521 cgattttgcc ttcgatttta taaaggtttc ggctcggaat cgtcatattg atttcgacta
     2581 tttcctgacc gagctcgaga ttaatgatgt ttgccgcttt cggctcgaaa acatcgggat
     2641 aaaacatact caaaagagag tttccgctgc cgaacatagt ctcgaactcg ttgtaagacc
     2701 ttttttcgcc gctttcgtaa tgcgaaacgc tctcggtcac tttgacgata tattcgcccg
     2761 ccggcagacc ggaaaaacgg taaattccgc ggtcgtcggt ctggtaggag ccgctgcccg
     2821 tcatcatcga gaaaatcgcg ctgaaattag gaatgacggg cataaattta ttattcacct
     2881 tgcgcagagc ctcgaccttg acgccgattg ccgggtcgcc gttgtcatac ataagccgcc
     2941 cgccgatagc gccgccgcga cgggcgggaa tctgcacgtc gaggtcggtt atcccgtcga
     3001 cgattatttt ttcaaatccc tcggcagcct tttcaaattc ttccgcttcc gacgctccgc
     3061 cccgcgcctt cgaaaaatcc ataaaagcca gcggcgagac gacgcccggc gcgttgacca
     3121 tcgcgtaata aatgccggct tgaacgccct tcatctgaaa attcccgtca ttatcggtca
     3181 agccggtcgg cgaggtgccc ggtccgccca agcctttttc ccgcatcagc ataatgggcg
     3241 cgcgcttgac cgctctgccc gtatcggcgt aaaaaacttt tccgcggacg gtcgaattgg
     3301 cgggcgtcgg cgtcggaaca tcgcttgttg ccgtgttgcc gattcccgta acctctctgg
     3361 tttccatcac tatttccgca ttattagtcg aagctttctt tttcggtcgc ggcgtcgccg
     3421 tgcgtttgac ggtcgattgc ggtttcggcg gcgtgttttg caaaaaagcg gtttccgtca
     3481 aaaccgaaaa accgaacacc aagccggaaa agcaaagaaa aatctttaaa aaaagcaagc
     3541 gcatattgtt ctggtgcagc atttcaagac tccggattca gagaaaattc gctctcgtta
     3601 aaacgaaaag ccgttctgcg cgagtataaa gcaaaacggc ttcggtttct aatttaatta
     3661 gaaaatctat ctcggttttc tgccgctcag aagcagctcg cgcccgacat agtaaagtaa
     3721 aatcgccagt atccagaacg gaatcagccc cagccaatcg gaattcggac cggcgaaaaa
     3781 cgggttgttg gcttgcgacc atgtctggaa ggcatccatt ctcttactca aaaactcggt
     3841 cgctacggct agtaagatgc ctgccaaagc tccgcccgcg atgtagcccg acgccagcag
     3901 aacgcccgga cttttatcgg tttcggcgat tatttcgtct tcggttaaat ttcggttagc
     3961 cagttttttc tttaagtaaa tatcgaccgc ccagcgcacg attccgccga cgaaaatcgg
     4021 cgcggagacc gaaatcggca gataaagccc gaccgcaaac gccagcgacg gaattcccgc
     4081 cagctccagc acaaccgcga tcatcacgcc taaaagcacc aatccccacg gcagctgctg
     4141 gctcaaaata cctttgatga tatagctcat cagagtcgct ttcggcgggt catacttgac
     4201 gacttccgtg ttgtcggcgc gtctggtcac cgtgccgccg atgcccggat caaccagata
     4261 aacgggcttg ccctgcgtat cgatcagata acgaccgggt tttccgttag cggaatccgt
     4321 gttgtgccag actcgatatg tattctggtc gttaaaatag gcgtcggcaa ccttttcgcc
     4381 tttcagctgc gcgccgtcgc gcgacaattg ttgttcggga acgacgaatt gctggctgaa
     4441 agccgccggc tcgacctttt gataaaccgt cgaagaagta ttcagctgaa tcagaatcgg
     4501 accgagcagc agagctgacg ccgtcgcgcc gatcagaatg gcgatttgct gtttccaggg
     4561 cgttccgccg acccaaaatc cggtttttaa atcctgcgaa gtcgtgccgc cgtttgaagc
     4621 ggcgatacaa acgatgccgc cgatactcag agccgtgaca aaatacgggt cgggctcgac
     4681 ccagccgata atcagaaaaa ccagacaagt caaaagcaga gtggcgacgg tcattccgga
     4741 aatgggattt gacgacgagc cgatttcgcc cgtcaggcgc gacgaaacgg tgacgaataa
     4801 aaacccgaaa atgacaatca gaatcgcgcc gagcaaattc atttttaaag ccggcgcgag
     4861 cgtgataacg ataattaacg ctaaaatacc gataaccacc catttcatcg aaaggtcgcg
     4921 atccgtgcgc ggcgcgttgt cggtttcggc ggcgctgctg ctgccggtgc tgccgcgaaa
     4981 atcggcgagt ccgcccttta agccgtgcca aatcgtcggc aggctgcgcg ccagcgaaat
     5041 cattccgccc gccgccacgg ctccggctcc gatgtataaa acgtaactgc tttgaatgtt
     5101 tctgatgcct gcggcgaaat taaagccgcc gccgtcggca accggaatat aaaacaaatc
     5161 gtgaatcgtt ttcgtggttt ccggcgcgag cggcgtctgc aaaaattcgc cgaaataaac
     5221 gatcatcggg attaatacga gataagacag cgcgccgccc gcaaacataa ttccggcgat
     5281 tttaggtccg atgatatagc cgacgccgag cagcgccggg ttattttcca gcgagatcga
     5341 gccgcctctg aaaggcgcgt cgaattgttt cccgggaacc tctttccagc ctttgaaaac
     5401 ctgcatcagc gtattgaaaa gaaaaccgat gccgaaaccg atggcgataa cttttccgcc
     5461 gttggtcgct gattcgtccg cgcgcacctt ttcggattcg atagacgcgg cgcgcgattc
     5521 ttcgcttgct cccgcaatca gaacctgcgc gcaagccgtt ccttccggat attttaaaat
     5581 tccgtgctgg tctttaatca aagcgcggcg cagcggaatc atcatcagga ttcccagaag
     5641 cccgccgagc acggcgacca gcatcacgcg cgaaagctcc aggtcgaagc cgagaatcag
     5701 aatcgcgggc atcgtcacgc ccagaccgaa agcgatcgat tcgcccgccg agccagccgt
     5761 ctgcacgata ttgtgttcga gaatcgtcgc gtcgcgcgtg attcgcgctt tcgacaaaag
     5821 tcgaaacagc gttatcgaaa tgacggcgac gggaatcgac gcgctgaccg tcaagccgac
     5881 cttcaaaacc agatacagcg acgacgcgcc gaaaacgacg ccgagaagcg ttccgacaat
     5941 cagcggaaaa atcgacaact cgggcatatt cgtcaccgcg gcgggaatat acgggctaaa
     6001 tttttcgaga aagggatttc tcttcactgg aaaagcctcc ttgaattttg taaaaacggg
     6061 gaaagttttt tttgaattga aacttgcgtt aatttaactt gccgaagccc tttgtggcaa
     6121 gcggtttatc agaaaacaag tcgaaaaacg gcttttcggc gaaaaaaaga ttttttttct
     6181 t
//
LOCUS       DASWNE010000813         1831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_204475, whole genome shotgun sequence.
ACCESSION   DASWNE010000813 DASWNE010000000
VERSION     DASWNE010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204475"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..1243
                     /locus_tag="VGB68_15910"
     CDS             89..1243
                     /locus_tag="VGB68_15910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEY0050778.1"
                     /translation="MPTRIDDLLRMAMSFGASDLHLRAGSFPVIRVSGELRPVSGVSR
                     LAQEETLEMAFSMMSNRQKQHFKEAYEVDIGYSVSGLGRFRVNIFQQRGSVGIVARVI
                     PDTIRNFAELGLPPVLSKIAEENRGLILVTGTTGSGKSTTLAAVVDYINQTRNTHIVT
                     VEDPIEFLHKDKKSFVTQREVDVDTRNFAEALRGSLRQDPDVILVGEMRDLETIETAL
                     VAAETGHLVLSTLHTLDASETIARIISAFPPFQQKSIRIQLAGLLKAVVSQRLMKEAN
                     GKGRVPAVEVLVSTPLIRDYILNEEKTAQVRDAISAGTSQYGMQTFDQSLFYLYQSGL
                     ITLEEALRGSTNPDEFRLRLAGIQNTTTIAREDMERQSGVTKVSDLISRI"
     gene            complement(1363..>1831)
                     /locus_tag="VGB68_15915"
     CDS             complement(1363..>1831)
                     /locus_tag="VGB68_15915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HEY0050779.1"
                     /translation="LGLNLADLNVSAGLPVQVISTGIKALGAPVVSLDALGRCRINSS
                     LLSEIYLSYGAIGCYAFTFETMEDASRIHARFFAPDDNIPEDPATGSAAGSLSGYLVY
                     HAALDAKKFTIEQGDFMQRPSRIHAEVTGEKGNIEKVRIGGQSVVVARGEVYL"
BASE COUNT          438 a          505 c          463 g          425 t
ORIGIN      
        1 cagttaaaat ctattaggta gtttggtatt tcctgtccag acgtttaaaa tctgtcccaa
       61 cccttggaaa agtttctatt cttcttgtat gccgaccaga attgatgatt tattgcggat
      121 ggcaatgagc tttggcgctt cggacttgca cctcagagcc ggttcttttc ccgtgattcg
      181 cgtgagcggc gagctgcgtc ccgtttcggg cgtctcgcgt ctggcgcagg aggagacgct
      241 ggaaatggct ttttcgatga tgtcgaatcg ccagaaacag cattttaagg aagcatacga
      301 agtcgacatc ggttacagcg tttcgggctt gggcagattt cgcgtcaata ttttccagca
      361 gcgcggctcg gtcggcatcg tcgcccgcgt cattcccgac acgattcgca atttcgccga
      421 gcttggactg ccgccggttc tgtcgaaaat cgccgaagaa aatcgcggct tgattctggt
      481 tacgggaacc accggctcgg gaaaatcgac gacgctcgcc gccgtcgtcg actacatcaa
      541 tcaaactcga aacacgcata tcgtcacggt cgaagacccg atcgagtttc tccacaagga
      601 caaaaaatct ttcgtcacgc agcgcgaagt cgatgtcgat acgcgcaatt ttgccgaagc
      661 tttgcgcggc tctctgcgac aagacccgga cgtgattctg gtcggcgaaa tgcgcgatct
      721 cgaaacgatt gaaaccgcgc tggtcgccgc cgaaaccgga catctcgttt tatcgacttt
      781 gcacacgctc gacgcttcgg agacgattgc gcgcatcatc tcggcttttc cgccgtttca
      841 gcagaaatca attcgcatcc agctcgccgg acttttaaaa gccgtcgttt cgcagcgcct
      901 gatgaaggaa gcgaacggca aaggcagagt tccggcggtc gaagttctcg tttcgacgcc
      961 tttgattcgc gactacatac tgaacgagga aaaaaccgcg caagtccgcg acgccatctc
     1021 ggcgggaact tcgcaatacg gaatgcagac gttcgaccaa tcgctgtttt atctttacca
     1081 atccggtctt atcacgctcg aagaagcttt gcgcggctcg accaacccgg acgaattccg
     1141 cctgcggctc gccggaattc aaaacacgac gaccatcgcc cgagaagata tggaacgcca
     1201 gagcggcgtc accaaagtca gcgatttgat ttcgcgaatc taaaaagttt acgggaagtt
     1261 tgcagacagt ttgcggatag tggcagatag ttaaatcaat cttgtaacgt ctgactctaa
     1321 aactatctgc cacttcccgc aactctgtaa acttcccgta aactataaat aaacttcgcc
     1381 tctggcgacg acgaccgatt gccctccgat tctgactttt tcaatatttc ctttctcgcc
     1441 cgttacttcg gcgtgaatcc ggctcgggcg ctgcataaaa tcgccctgtt caatcgtaaa
     1501 ttttttcgcg tcaagcgccg cgtgatatac cagataaccg gaaagcgagc cggcggcgct
     1561 gccggtcgcc gggtcttcgg gaatattatc gtcgggcgca aaaaatcggg cgtgaatgcg
     1621 tgaggcgtct tccatcgtct caaacgtaaa cgcgtaacag ccgattgcgc cgtaggaaag
     1681 ataaatttcc gaaagcagcg acgaattgat tcggcaacga cccaaagcat cgagcgaaac
     1741 gaccggtgcg cctagcgctt tgatgcccgt cgaaatgacc tgaacgggca aaccggcgct
     1801 gacattcaaa tccgccaaat tcagtcccaa g
//
LOCUS       DASWNE010000814         2056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_592799, whole genome shotgun sequence.
ACCESSION   DASWNE010000814 DASWNE010000000
VERSION     DASWNE010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592799"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VGB68_15920"
     CDS             complement(<1..469)
                     /locus_tag="VGB68_15920"
                     /inference="COORDINATES: protein motif:HMM:NF014336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HEY0050780.1"
                     /translation="MEINMDDSISLFNRAIAAEFFWSIPRHQIIADAAQKLLKQNQTA
                     LDEIGRILKPLKTDRLAAVAGWADTVKRRKPNPAKDDSFTVEFLESPKYKELNALNDT
                     WHYVNLPLDADAYDREKYKNFTRDDDVVQILKEAIRVLKTKGKHDRFSEVNALR"
     gene            complement(765..1436)
                     /locus_tag="VGB68_15925"
     CDS             complement(765..1436)
                     /locus_tag="VGB68_15925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050781.1"
                     /translation="MDELRRPFFIVALVLMFIVVLLEIGSPWLLEPIKGDFDRPGYGI
                     QYLVFVDAFLLYVTVLIGLALIIPERIQGRLQGCATLIISFFGCLGIIGAIIFAIVFL
                     LLLVTLLLSPIFGTIAYLAVYGDFDRTGANITLSLIMLLKIGYCIFLVLAHQRFLQNK
                     SLVLLTLTSLAANIIISFLHNLLPLILVSITDTLGAIVVAILALIWSIIFLIGSIIAI
                     VKSIG"
     gene            complement(1438..1944)
                     /locus_tag="VGB68_15930"
     CDS             complement(1438..1944)
                     /locus_tag="VGB68_15930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050782.1"
                     /translation="MNKIVIIVLVLIAAVFFIFVGVGSFESEPEKDPKKVKAPPGSET
                     LNNLFGGLQEKIVLRCDPASSANSSLQCQKLLMGTANIPPAKEPSLPFLKKTTFRTAK
                     IVLLSGQATIFYRDNKGISGQDNPRITNLTNPNDKNSNVESIVVLERGGTLNISCIGN
                     TSCQVGQQ"
BASE COUNT          551 a          484 c          483 g          538 t
ORIGIN      
        1 ggcgcagcgc gttgacttcg ctgaagcggt cgtgctttcc ttttgttttc aaaacccgta
       61 tagcttcttt caaaatctga acgacgtcgt cgtcgcgggt aaagttttta tatttttcgc
      121 ggtcgtaggc gtcggcgtcc agcggcaggt tcacgtaatg ccacgtatcg tttagcgcat
      181 ttaattcttt atacttcggg ctttccagaa attcgacggt aaacgaatca tctttggctg
      241 gattgggttt tcgtctttta acggtatcag cccagccggc gacggcggcg agccggtccg
      301 ttttcagagg tttcaggatt ctgccgattt cgtccagagc ggtctggttc tgctttaata
      361 atttctgagc ggcgtcggcg ataatctgat gtcgcggaat gctccaaaaa aactcggcgg
      421 caattgcgcg gttaaataaa gagatgctgt cgtccatatt gatttccatt tttttgctga
      481 ttgaagcgat tactaaaaat tcagagtcgg attggtaaat aatcggatta tgaatttttc
      541 gactgagcct gtttacaatc ggagccgcgc ggggcggcat cgggtcttaa actgtatttt
      601 ttacggcaat attccaacga ggaagattca gggaaaattc ccgcaaagca tttattgtgt
      661 tgtcggttga tagaaattat cacaggtcaa atttgcctgt caacgatttt ttcgaaccgg
      721 ttccttcggt tgaaatccgc cgtcaaaaaa ccgcctcggc tttctcaacc gatagatttg
      781 acgattgcga taatcgaccc gattaaaaag ataatcgacc aaatcagcgc cagaatcgcc
      841 accacaatcg cgccgagcgt atcggtgatg ctgaccagaa tgagcggcag cagattgtgc
      901 agaaaactga taattatatt tgccgcgagc gaggtcagcg tcagcagaac gagacttttg
      961 ttttgcaaaa agcgctggtg agccagaacc agaaaaatgc agtagccgat tttcaaaagc
     1021 attatcaagc tcagggtaat gtttgcgccg gtgcggtcga aatctccgta aaccgccaga
     1081 taagcgatgg ttccaaaaat cggcgaaaga agcagagtaa ccagcagaag cagaaaaaca
     1141 atcgcgaaaa taatcgcgcc gataattccc agacacccga aaaacgagat aatcagcgtc
     1201 gcgcagccct gcaatctgcc ttgaattctt tcgggaatta tcaaagccaa gccgatgagc
     1261 accgtcacat acaacagaaa agcgtcgacg aaaaccagat actgaatgcc gtagcccgga
     1321 cggtcgaagt ctcccttgat aggttcgagc agccacggcg agccgatttc aagcagcacg
     1381 acgataaaca tcagcaccag cgcgacgata aaaaacggtc tgcgaagctc atccatttta
     1441 ttgttgtcct acctgacacg aagtatttcc gatacaggaa atatttaaag ttccgcccct
     1501 ctccagaacg actatcgatt cgacgttcga attcttatca ttcggattcg tcaaattagt
     1561 tatccgggga ttatcctgcc cggagatgcc tttgttatcc ctgtaaaaga ttgtcgcctg
     1621 tccgcttaac aggacgattt tggcagtgcg aaaggtggtt tttttcaaaa aaggtaaaga
     1681 cggctctttc gcgggcggaa tatttgcggt tcccatcaat aatttctggc attgcagcga
     1741 cgaatttgcc gacgatgccg ggtcgcatct caaaacgatc ttttcctgca atccgccgaa
     1801 aagattgttt aaagtctcgc tgcccggcgg cgctttgact tttttcgggt ctttttccgg
     1861 ttccgattcg aaacttccca ccccgacgaa aataaagaag acggcggcga ttaaaaccaa
     1921 aacgataatg acgattttat tcatatctac caatcaatta cggtcaattt cggaaaacta
     1981 cggtgttttt cgcctcgaag cgacgactcg ccgcgcacgc gtagctattc gatttttatc
     2041 ccggaaataa aattcg
//
LOCUS       DASWNE010000815         2436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_131543, whole genome shotgun sequence.
ACCESSION   DASWNE010000815 DASWNE010000000
VERSION     DASWNE010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131543"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VGB68_15935"
     CDS             complement(<1..718)
                     /locus_tag="VGB68_15935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050783.1"
                     /translation="MFSHFKTFGLVVSLCLASVLSAAAQEDKTRDKPKKEEKPKQQQQ
                     QANDISKLTTADQVAESTIVIYGGLVGRQHLHQIRRTTVEVGRTAIVNAEGKTDQIPY
                     IKRILRGENLEKERIRIDQEISGSRYSWIYNGGTFVGLFNETVFTPKEETLSVFQNQL
                     WHGLEALLRYKENGATVTLGGREKYMNVEYYILDVTDKQNRQTRFFISTKTLRVMALE
                     YTQNAVKYARRFYDYRYAQGT"
     gene            894..1508
                     /locus_tag="VGB68_15940"
     CDS             894..1508
                     /locus_tag="VGB68_15940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050784.1"
                     /translation="MSEKIQNLLKEAETSDDGKSSVTSAEREFASAEEAVEAFDLFTK
                     KLYDINRWEADSGVSAFQLFDENGKGLETPKAQKGNFVRISLPGSGKYDWVKIVDIRD
                     ATDEIVITVQPTYDPTAKETDKDTTSHFFTSESTNNFCLQKAGKSLAVYVIGLHEKPN
                     TSESGGVIESVRNTAAATFGWLGFQKIEWKTFCENFLETEKMKK"
     gene            1583..2044
                     /locus_tag="VGB68_15945"
     CDS             1583..2044
                     /locus_tag="VGB68_15945"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEY0050785.1"
                     /translation="MLHIALVEPEIPPNTGNIARLCAASFTSLHIVGVAGFRLDDRAV
                     RRAGLDYWDEVRLFRHRHLNALYEALPDSRFLYLTTKTDKLYTDFAFQDGDCLVFGRE
                     TRGLPEDLLKANWERCLTIPMPNPKIRSLNLATSAGIVLYEALRQVGFFTK"
     gene            2147..>2436
                     /locus_tag="VGB68_15950"
     CDS             2147..>2436
                     /locus_tag="VGB68_15950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY0050786.1"
                     /translation="MKFIRHFLILAVAIFGVSAVSASAQRYADNDKRNSSRTLEQKVF
                     KQLIKLPYYGVFDNIKFELSGNTVILSGKVAVARNYDDAERAVKGITGVQRV"
BASE COUNT          617 a          612 c          594 g          613 t
ORIGIN      
        1 gcgtgccttg cgcgtagcga taatcgtaaa accggcgcgc gtatttgacc gcgttctgcg
       61 tgtattcgag cgccatcacg cgcaaggttt tcgtgctgat aaaaaagcgc gtctggcgat
      121 tttgcttgtc ggtcacgtcc aaaatgtaat actcgacgtt catatatttt tcgcgcccgc
      181 cgagcgtcac cgtggcaccg ttttccttgt atcgcaaaag cgcttcgagc ccgtgccaga
      241 gctgattctg aaaaacgctg agcgtttcct ccttcggcgt aaaaacggtt tcgttaaaaa
      301 gtccgacaaa cgtgccgccg ttataaatcc acgagtagcg cgaaccggaa atttcctggt
      361 cgatgcggat gcgctctttt tcgagatttt cgccgcgcag aatgcgtttt atatacggaa
      421 tctggtcggt cttgccctcc gcgttgacga tggcggttct gccgacttcg acagtggtgc
      481 ggcgaatctg atgtaaatgc tgtcgcccga ccaatccgcc gtaaatcaca atcgtcgatt
      541 cggcaacctg gtcggcggtc gtcagcttgc ttatatcatt cgcttgctgt tgctgttgtt
      601 tcggtttttc ttcctttttt ggcttgtctc gcgttttatc ttcctgtgcg gcggcagaca
      661 aaacacttgc cagacataac gaaaccacca aaccgaaggt cttaaagtgt gaaaacactc
      721 attgaaatct ccttagaact tgtggaattt gacactcgcg gattttagca caaactggaa
      781 aattcggcac actccgctat tttaaactat ctgaattgat gaaaattagc ttaatttaga
      841 tgcttcaaag aaaatacttg tcagacaatt ttttgcggct tactatagat aaaatgagcg
      901 aaaaaattca gaatttacta aaagaagccg aaacttccga cgacggaaaa agctcggtga
      961 ccagcgccga acgcgagttt gcgtcggctg aggaagccgt cgaagccttt gatttattca
     1021 cgaaaaagct ttacgacatt aaccgctggg aagccgattc gggcgtttcg gcgtttcaat
     1081 tatttgacga aaacggcaag ggtctggaaa cgccaaaggc gcaaaaagga aatttcgtgc
     1141 gcatttccct gcccggctcc ggcaaatacg attgggtgaa aatcgtcgat atccgcgacg
     1201 cgacggacga aatcgtgata accgtccagc cgacctacga cccgaccgca aaggaaacgg
     1261 acaaagacac cacgtcgcat ttttttacga gcgaatcgac taataatttc tgtctgcaga
     1321 aagccggaaa gtcgctggct gtttacgtca tcggtctgca cgaaaagccg aatacatccg
     1381 aaagcggcgg cgtcatcgaa agcgtccgca acacggcggc ggcgaccttc ggctggctcg
     1441 gttttcaaaa aatcgaatgg aaaacctttt gcgaaaactt tctggaaact gaaaagatga
     1501 aaaagtgaaa gcgtgaaaaa gccgcttgac ggcgctcagc agatttttca ctttttcacg
     1561 ctttcacttt ttcgctttaa aaatgctgca tatcgccctg gtcgagcctg aaattccgcc
     1621 gaataccggc aatatcgcgc ggctgtgcgc ggcgagcttt acttcgctgc acattgtcgg
     1681 cgtcgccgga tttcgtctgg acgaccgcgc cgtccgccgc gccggtctgg attactggga
     1741 cgaagttcgc ctttttcgtc atcgtcattt aaacgcgctt tacgaagcgc tgcccgattc
     1801 gcgttttctt tatctgacca cgaaaaccga taagctttac actgatttcg cgtttcagga
     1861 cggcgattgc ctcgtcttcg ggcgcgagac gcgcgggctg cccgaagatt tgctcaaagc
     1921 caactgggaa cgctgtctga ccatcccgat gccgaacccg aaaatccgca gtttgaacct
     1981 cgccacgagc gccggaatcg tgctttacga agcgctgcgg caagtcggat tctttacaaa
     2041 ataatcaaga ttacctgcat cttttcagca atttctcaca tcaaatccgt cgtaacaagt
     2101 gagtaatcaa attcataaat ttattcttga ttacgaggga gaacatatga aattcataag
     2161 acatttttta attctggcag tggcaatctt cggcgtttcg gcggtcagcg ccagcgccca
     2221 gcgatatgcc gacaacgaca aacggaattc ttcgcgaacg cttgagcaga aggttttcaa
     2281 acagttgatt aaactgccgt attacggcgt ttttgacaac atcaaatttg aattaagcgg
     2341 caacaccgtg attttgtccg gcaaggtcgc cgtggcgaga aactatgatg acgcggaaag
     2401 agccgtcaaa ggcatcacgg gcgtgcagcg ggtcat
//
LOCUS       DASWNE010000816         1797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_275381, whole genome shotgun sequence.
ACCESSION   DASWNE010000816 DASWNE010000000
VERSION     DASWNE010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275381"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VGB68_15955"
     CDS             complement(<1..605)
                     /locus_tag="VGB68_15955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050787.1"
                     /translation="MEAKICINCGKTKSLISFASNKKKDGTISYASKCRSCSTGTTPL
                     NSPFFDESKNQRVKLCKHCQQIKPVDEFKKGKYIGNCKNCTNEQNKISYAQKSPEEKK
                     LKAKIYGAKQRENKEIYNAKRREYYAKPEVKKRLLEAQRKRYKSCAGQKTKERYLKYK
                     ESIKKGMAAYQARKKGASVVVRFTKADIIKRDGLNCYLCDK"
     gene            complement(725..1147)
                     /locus_tag="VGB68_15960"
     CDS             complement(725..1147)
                     /locus_tag="VGB68_15960"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY0050788.1"
                     /translation="MNTKICKNCQLEKPLQMFAVKRGDFWSYAERCRPCRKLAKPFQR
                     FWKPLSPEICERNKMTRRARLAGASVIIPFTKAEIIRRDGLNCYLCGKLLTYKSATID
                     HVIPLSRGGYHAPSNAKIACFPCNRAKYNKNLQEFLNG"
     gene            complement(1128..1328)
                     /locus_tag="VGB68_15965"
     CDS             complement(1128..1328)
                     /locus_tag="VGB68_15965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050789.1"
                     /translation="MSCPKCHKNYTCADCRKKWIAERREKSANVESLELARLFVRTRN
                     GQINFGEFKQLIFSLQNEHENL"
     gene            complement(1325..1636)
                     /locus_tag="VGB68_15970"
     CDS             complement(1325..1636)
                     /locus_tag="VGB68_15970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050790.1"
                     /translation="MKKKTKYRKGKFPYTVLNTENGKLYVRKSVTLKGKQHQIWRVCE
                     PPISERAAEILEEIRLEVQAENSLIGYSKKDAELARVFMQVRDKEIGFFHFKQKVAIK
                     K"
BASE COUNT          404 a          402 c          352 g          639 t
ORIGIN      
        1 aatttatcgc aaaggtagca attcaatccg tcgcgcttga taatatcggc tttggtaaat
       61 ctgaccacca cagacgcgcc tttctttcgc gcctgataag ccgccattcc ttttttaatt
      121 gattctttat attttagata gcgttctttt gttttttgac cggcacaaga cttgtatcgt
      181 tttctttgag cctctaagag tctctttttc acttcgggtt ttgcgtaata ttcccgcctt
      241 ttagcattgt agatttcctt gttttctctc tgtttggcac cgtatatttt agcttttaat
      301 ttcttttctt cgggagattt ttgagcgtaa gaaattttgt tttgttcgtt ggtgcagttt
      361 ttacagttgc caatatattt tcctttttta aattcatcga caggcttaat ttgttggcag
      421 tgtttgcaga gttttacccg ctgatttttt gattcgtcaa aaaacgggct gtttagcggc
      481 gttgtgcctg tggaacaaga cctgcattta ctcgcataag aaatagttcc gtcctttttc
      541 ttatttgaag caaaagatat taaagattta gtttttccgc agtttataca aattttcgct
      601 tccataatca cctttgatat aaatttttcg cgctgttaaa aaatcgcctt tcgctttaaa
      661 tttcgcttcg cagagccagc aacggcgcgg cgaatcttgg aagttatgcc gaaaacattt
      721 gaatttatcc atttaaaaat tcctgtaaat ttttgttata tttcgccctg ttacaaggaa
      781 aacaggcgat tttcgcgttg ctcggcgcgt gatagccgcc ccgtgaaagc ggtataacgt
      841 ggtcaatcgt agcggattta taagtcaata attttccgca aaggtagcaa ttcaaaccgt
      901 cgcgccgaat gatttccgct ttggtaaaag ggattatcac gctcgcgccc gccagtcgcg
      961 cccgccgcgt cattttgttt ctttcgcaaa tttccggcga taatggtttc cagaatcttt
     1021 gaaacggctt cgccagtttc ctacacggtc ggcatctttc ggcataactc caaaaatcgc
     1081 cgcgcttgac cgcgaacatt tgcaaaggct tttccagctg gcagttttta caaattttcg
     1141 tgttcatttt gcagagaaaa aattaactgc ttaaactcac caaaattgat ttgaccgttt
     1201 cgcgttctga cgaacagccg cgccagttcc aaagattcga cgtttgccga tttttcgcgc
     1261 cgctcggcaa tccatttttt acggcaatcc gcgcaagtgt aatttttatg acatttcgga
     1321 caactcattt ttttatcgct accttttgct taaagtgaaa aaagccgatt tccttatccc
     1381 gcacctgcat aaagacccgc gccagttccg cgtctttttt gctgtatccg attaaggagt
     1441 tttccgcttg aacttccagc cggatttctt ctaagatttc ggcggctcgc tcactgatcg
     1501 gcggctcgca gacgcgccaa atttgatgtt gcttgccttt aagcgtgacg ctttttcgga
     1561 cataaagctt tccgttttcg gtattgagaa ccgtgtaagg aaatttgcct tttctgtatt
     1621 tcgtcttttt cttcataaat ttaagcgagg cgtttcagcc gtaatttttg gtcgggctta
     1681 tcgcgcctcg tttgaaaaga gattttttgt tatctacgaa ataactctct tttcgttttt
     1741 ccactatcgg tttatggctt tttcagacgt ccaatttcgt tcccgaaatt tcaggct
//
LOCUS       DASWNE010000817         7305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_97459, whole genome shotgun sequence.
ACCESSION   DASWNE010000817 DASWNE010000000
VERSION     DASWNE010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7305
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97459"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1640
                     /locus_tag="VGB68_15975"
     CDS             <1..1640
                     /locus_tag="VGB68_15975"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEY0050791.1"
                     /translation="AFASSRKFLWDAQGHTVGGNKVMAQSLYPKEASPLWDKFSTHAI
                     IHTLNVYSRYSFDYPYPSAISVSNRHVGGMEYPMICFNGPRPNPDGTYSSNAKYGLIG
                     VVIHEVGHNYFPMIVNSDERQWTWMDEGLNTFLQYLAEQEWEKNYPSWRGEPKNITEY
                     MLSENQVPIMTNSESVLQFGNNAYGKPATALNILRETVLGRDLFDFAFKEYSRRWKFK
                     RPQPADFFRTMEDASGTDLDWFWRGWFYSTDHVDISIENVRLYQMDTKNPEVEKELQR
                     KQANAEPRTLSQQRNEALPKRIDQYPSLRDMYNDTENNRFRVTEQDRQTYQAFYSSLS
                     DKEKALVNGNYHFYVVDLKNVGGLVMPLIFRVEYMDGTSEEVRVPVEIWRYNNWEVSK
                     LIVTRKEAKAIVLDPNLETADADLANNFFPRRAAPTRFQIFKQGQPNRAQTTTTTPGA
                     ANIAGRWNIAVDAGGQTINVVLNLTQQGNAISGTIESAQTGTIQISSGVMENGRFTLK
                     TTTPVELTFTGQATGNQIAGSVVSPQGTTTFTGSKAN"
     gene            1684..2235
                     /locus_tag="VGB68_15980"
     CDS             1684..2235
                     /locus_tag="VGB68_15980"
                     /inference="COORDINATES: protein motif:HMM:NF042765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6702 family protein"
                     /protein_id="HEY0050792.1"
                     /translation="MKRTSIHKNQIFRFAGVLLLFSLFTFHFSLFTVSAHRYHTSLTR
                     LDYNEKEKLVEISIQLFTHDLVPALEQKAGKRVDLEKTPEVDKLILGYLNENFVLRNK
                     NGESTALKWVGKELEVDAIWVYVEIPSTENPAGANLQNTILFESFPEQTNLVIARFGG
                     KKADLMFKVGDKFKEIRENAPKE"
     gene            2563..3402
                     /locus_tag="VGB68_15985"
     CDS             2563..3402
                     /locus_tag="VGB68_15985"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEY0050793.1"
                     /translation="MKLALNGATTMHADLETDIRAASAAGFDLIEIWAAKLRRFLKTN
                     TAADLKKLLKENDLQAYSINSIEHITFRTNDEYEKIKAETEELSQIAGELECPFVVVV
                     PGKLPENATKEEIVNESVRVLNELADIAGKHNVGLAFEFLGQTDCSVQTLDLCNEIVE
                     KVNRPNVGNVIDTFHFYAGGSSFEAIENMKPEKLFIFHINDAEDLPKEQLTDAHRLYP
                     GTGILPIREIKERFDKIGYNRMVSIEIFRPEYWNENPFEVAKKAKEATEKVLGLTPKG
                     ATA"
     gene            3549..4520
                     /locus_tag="VGB68_15990"
     CDS             3549..4520
                     /locus_tag="VGB68_15990"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY0050794.1"
                     /translation="MNKVKIGIIGTGYIGNVHARIHSRDERAEVAALYDIVPEKAERA
                     AKSVGGKVCRSLEELFAATDAILVCTPNKTHVEIASRAVAAGKHVFCEKPFAIGLDNA
                     RVLLETARNGTGVFQVGHNRRYAPVYAKLKELLAKDAAHSAHIKMNRGELKNPVWTGD
                     PDVTGGFLYETTIHLFDMMRFQFGEIEELVAYGSTHEYPEIDEFSIIFKFKNGFHSTF
                     ASSSDASWLFPFERIEVFCHHRTILTQEMEHLIDSRGCDANFEALSFHMTEKEERWGY
                     VQEDRAFIDAILNETEPPVTAFDGYKSVELVESVYKAIQTGEKIRFE"
     gene            4759..5238
                     /locus_tag="VGB68_15995"
     CDS             4759..5238
                     /locus_tag="VGB68_15995"
                     /inference="COORDINATES: protein motif:HMM:NF022070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LITAF-like zinc ribbon domain-containing
                     protein"
                     /protein_id="HEY0050795.1"
                     /translation="MVQCQNCGQVNNYGSNFCRFCGTKMVVGQQSQPPQQQQQNQSYE
                     YNPPKPYSWKTDEFQVQDAKHQQRKTQEINRVQPLSNFNQPPPQHLAYQQSPSMTHGY
                     RCPRCGTQNLPHVERKISTAGWIVFAALLLVFFPLFWIGFLIKEDVRVCPVCSLKVG"
     gene            complement(5299..6267)
                     /locus_tag="VGB68_16000"
     CDS             complement(5299..6267)
                     /locus_tag="VGB68_16000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050796.1"
                     /translation="MFKMIARKFFVVFVSTLLAANFCLAQPENENNQKKQQKVRTVTI
                     PISIFSKEELKQQIPQEFIEAGNIIVREDGDQQTILSIRSIGEAPMALAVLIQDDVSS
                     SVNLELKRLADFIRRLPKGSRVMVAYLRAGSIQVRQKFTEDLEKAAGSLRIIAGVASA
                     APSSPYQSVEEALGRFDALPIGRRAILLISDGLDASRGLDDSSPTGSLDLERAITKAQ
                     RKSVAVYSFYASASLTARASTTLILNAQGSLQKLSEQTGGRAFFQGLTTPVNFEPFFR
                     EVNLSLNRQFVLTYLSTHMKKGYHKVDVSSTNPEIKIEHPKGYYYR"
     gene            6379..7158
                     /locus_tag="VGB68_16005"
     CDS             6379..7158
                     /locus_tag="VGB68_16005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050797.1"
                     /translation="MNSLESQYYEVTRRDLSKDKALKFGAWVVPFVTAILPALVFFIL
                     FLFSSATPTAAMFFFLSLVSLVGGFIFGLIIGGGLMFYRSRWLKQIRERIAVDGIKAN
                     EVDFFQHELTSAERKSLREIENKDRMLADAYRETLASRLTATRILKTTRQELLLVRRR
                     EGKLKYLKSENSESLQKQLKDDQTKLDEIKTEAEQMRVQAETRLQMIEAASRRGTNFS
                     DTELALKRLTARSAELPLALEAIKMEEEIRRELEKEETENQ"
BASE COUNT         2029 a         1762 c         1802 g         1712 t
ORIGIN      
        1 tcgctttcgc ttcttcgaga aaattcctgt gggacgcgca gggacacacc gtcggcggca
       61 acaaggtgat ggcgcagtcg ctctatccga aggaagccag cccgctctgg gacaaattct
      121 cgacgcacgc gattattcac actttaaacg tctattcgcg ctactcgttc gattacccgt
      181 atccgagcgc gatttccgtc agcaatcgtc acgtcggcgg tatggaatat ccgatgattt
      241 gctttaacgg tccgcgcccg aacccggacg gaacgtattc gtcaaacgcc aaatacggct
      301 tgatcggcgt cgttattcac gaagtcgggc acaattattt cccgatgatt gtcaattccg
      361 acgagcgcca gtggacttgg atggacgaag gcttaaatac ctttctgcaa tatttagccg
      421 agcaggaatg ggagaaaaat tatccttcgt ggcgcggcga gccgaaaaat attactgaat
      481 atatgctttc ggaaaatcaa gtgccgatta tgaccaattc cgagtcggtc ctgcagttcg
      541 gcaacaacgc ttacggcaaa ccggcgaccg cgcttaacat tctgcgcgag accgttctgg
      601 gacgcgacct tttcgatttc gcatttaagg aatattcgcg ccgctggaaa ttcaagcgac
      661 cgcagcccgc cgattttttc cgcacgatgg aagacgcttc cggcacggat ttggactggt
      721 tctggcgcgg ctggttttat tcgaccgacc acgtcgatat ttccatcgaa aacgtgcggc
      781 tttaccagat ggacacgaaa aatccggaag tcgaaaaaga gcttcagcgc aaacaggcta
      841 atgccgagcc gcgcacatta tcgcagcagc gcaacgaggc gctgccgaaa cgcatcgacc
      901 agtatccgag cttaagagat atgtataacg acacggaaaa caatcgtttc cgcgtcaccg
      961 agcaggaccg ccaaacttat caggcgtttt attcgagcct gagcgacaag gaaaaagcgc
     1021 tcgtcaacgg aaattatcat ttctacgtcg tcgatttgaa aaacgtcggc ggcttggtga
     1081 tgcctttgat tttcagggtc gaatatatgg acggcacgag cgaggaagtc cgcgttccgg
     1141 tggaaatctg gcgttataac aactgggaag tttccaaatt gattgtgacg cgcaaagagg
     1201 cgaaagccat cgtgctcgac ccgaatctgg agacggcgga cgccgattta gcgaacaact
     1261 ttttcccgcg ccgcgccgcg ccgacgcgct tccagatttt caaacaaggt cagccgaacc
     1321 gcgcgcaaac aaccacgacc acgcccggcg cggcaaacat tgccggcaga tggaatatcg
     1381 cggtcgatgc cggcgggcaa acgattaacg tcgtgctgaa tctaacgcag cagggaaacg
     1441 cgatttcggg aacaatcgaa tcggcgcaga cgggcacgat tcagattagc agcggcgtga
     1501 tggaaaacgg caggtttacg ctgaaaacga cgacgccggt cgagttgacg tttaccgggc
     1561 aggcaaccgg caatcaaatc gccggctcgg tcgtctcgcc gcagggcaca acaaccttca
     1621 ccggttcaaa ggcaaactga aaacagtgaa aagcgaataa cgaaaagtga aaagttaaga
     1681 aatatgaagc gaacatcaat acataaaaat cagattttta gatttgcggg cgttttgctt
     1741 ctcttctcgc ttttcacttt tcacttttcg cttttcactg tttcggcgca tcgttatcac
     1801 accagcctga cgcggctgga ttacaacgag aaggaaaagc tggtggaaat ctcgattcag
     1861 ctttttacgc acgacctcgt gcccgctctc gaacaaaaag ccggaaagcg cgtcgatctc
     1921 gaaaaaactc ccgaagtcga taagcttatt ctcggttatc tgaacgaaaa ttttgttctc
     1981 agaaataaaa acggcgaatc gacggcgctg aaatgggtcg gaaaagagct tgaggtcgat
     2041 gcgatttggg tttacgtcga aattccctcg acggaaaatc cggcgggcgc gaatctgcaa
     2101 aacaccattt tatttgaaag ctttcccgaa cagacaaatc tggtcatcgc ccgtttcggc
     2161 ggaaaaaaag ccgatttgat gtttaaagtc ggcgacaaat ttaaggaaat ccgggaaaac
     2221 gcgccgaaag aataaagaaa aatcgccgtt taaaaagaga agaaagaagc ttgcgatttg
     2281 aagcttcttt ctttttctga ggtgagcggt gagatagacg gttcctcacg gacagacagc
     2341 aaccgacaaa tgctgcctgt aacggggttc cacttattaa tgactttttt gtcgatttat
     2401 tacagaactt tttaaaaaaa aagaaaaaag cggcaaacgg taagctcgtt tcttttttat
     2461 tgcaacttac cgtttaccgt ttaccgcttg ccgtttacca ctgcgaagcg gctcgtgtta
     2521 gaattttcac aaagtcaatc tttataaaat tcggagtaag aaatgaaatt agcattaaac
     2581 ggcgcgacga cgatgcacgc cgatttggaa accgacatca gggcggcgag cgccgccggt
     2641 tttgatttaa tcgaaatctg ggcggcaaag ctgcgccggt ttctgaaaac aaacaccgcc
     2701 gccgatttaa aaaaattgct gaaagaaaac gatttacagg cgtattcaat caattccatc
     2761 gaacacatca cttttcgaac caacgacgaa tacgaaaaaa tcaaagccga aacggaagaa
     2821 ttgtcgcaaa tcgcgggcga gctcgaatgc ccgttcgtcg tcgtcgtgcc gggaaaactg
     2881 ccggaaaacg ccaccaaaga ggaaatcgtc aatgaatccg tccgcgtttt aaacgagctt
     2941 gccgacatcg ccggtaaaca caacgtcggt ctggctttcg agtttctggg acaaaccgac
     3001 tgctcggtgc agacgctgga tttgtgcaac gaaatcgtcg aaaaagtgaa tcgcccgaac
     3061 gtcggaaacg tcatcgacac gtttcatttc tatgccggcg gttccagctt cgaagcgatt
     3121 gaaaatatga agccggaaaa gcttttcatc tttcatatca acgacgccga agatttgccg
     3181 aaagaacaat tgaccgacgc gcatcgcctg tatccgggaa ccggaatttt gccgattcgg
     3241 gaaattaaag agagattcga taaaatcggc tataaccgta tggtttcaat cgagattttt
     3301 cgccccgaat actggaacga aaatcctttc gaagtagcca aaaaagccaa agaagcgacc
     3361 gaaaaagttc tcggattaac gccgaaagga gcaaccgcat aagaagaatt caaacaggat
     3421 gcagtaggct tcgcggctgg ataaacagga taaattgttt ttcaaattaa gattcgagtt
     3481 ttattcaaag gtttgaataa aaaagctttt catcctgctt atcctgtcca tcctgttaaa
     3541 ttttcaatat gaataaagta aaaatcggaa tcatcggcac gggttacatc ggcaacgttc
     3601 acgcgcggat tcactcgcgt gacgagcgcg cggaagtcgc cgcgctttac gatatcgtgc
     3661 cggaaaaagc ggagcgcgcc gccaaatcgg tcggcggaaa agtttgtcgc tcgctggaag
     3721 aattgtttgc ggcgaccgac gcgattctgg tttgcacgcc gaacaaaacg cacgtcgaaa
     3781 tcgcttcccg tgcggtcgcc gccggaaaac acgttttctg cgaaaagcct ttcgccatcg
     3841 gtctggacaa cgcgcgcgtt ttgctcgaaa ccgcgcggaa cggcacgggc gtttttcagg
     3901 tcgggcacaa ccggcgttat gcgcccgtct acgcgaagct caaagaattg ttagcgaaag
     3961 acgcggctca ctcggcgcac atcaagatga atcgcggcga gctgaaaaat ccggtctgga
     4021 cgggcgaccc ggatgtgacg ggcggatttt tgtatgaaac gacgattcat cttttcgata
     4081 tgatgcggtt tcagttcggc gaaatcgaag agctcgtcgc ttacggctca acgcacgaat
     4141 atccggaaat agacgaattt tcgattattt tcaaatttaa aaacggtttt cactcgactt
     4201 ttgcttcgtc gtccgacgcc agctggctgt ttcctttcga gcgcatcgag gttttctgtc
     4261 atcaccggac gattttaacg caggaaatgg aacacctgat tgattcgcgc ggctgcgacg
     4321 caaatttcga ggcgctttcc tttcatatga ccgaaaagga agagcgctgg ggttatgttc
     4381 aggaagacag agcatttatc gacgcgattt taaacgaaac ggagccgccc gtgacggctt
     4441 ttgacggcta caaatcggtg gaactggtcg aatcggttta caaagccatt cagacgggcg
     4501 aaaaaatcag atttgaataa aaaatcagat ttgaataaaa aaatcagatt tgaataataa
     4561 atgaaactat aaacaaacta taaacaatgg aaacgatgaa tgctgaacga tgaatgcgca
     4621 aaatcgcatc ttccgcgctc gcgcgttcgt cgttccgcgt ttctttttcc cgctcggcgc
     4681 ttttaattaa agaacttttg ccgtaaagta tcgtaaaata tttcaggaaa gcttaaatat
     4741 cggattggaa gattcattat ggttcaatgt caaaattgcg ggcaagtcaa taattacggg
     4801 agcaatttct gtcgtttctg cggcacgaaa atggttgtcg gtcagcagtc gcagccgccg
     4861 cagcagcaac agcaaaatca atcttacgag tataatccgc cgaagcctta cagttggaaa
     4921 accgacgagt ttcaggttca ggatgccaaa catcaacagc gcaagacgca ggaaatcaat
     4981 cgcgtccagc cgctttcgaa ctttaaccag ccgccgccgc agcatttagc ctaccagcaa
     5041 tcgccgtcga tgacgcacgg ctatcgctgc ccgcgctgcg gcacgcaaaa tctgcctcac
     5101 gtcgaaagaa aaatttcgac ggcgggctgg attgtctttg ccgctctgct gctggtattt
     5161 ttcccgcttt tctggattgg gtttctgata aaggaagacg tgcgcgtttg cccggtttgc
     5221 agtctgaaag tcggttagga aagtgaaaag gtgaaaaagt ttgttgagtt tcaagttact
     5281 ttttcacctt ttcacttttt acctgtaata ataacctttc gggtgctcga ttttaatttc
     5341 gggattggtg ctcgacacgt cgactttgtg atagcccttt ttcatatgcg tcgaaagata
     5401 agtcaaaaca aattgacggt ttaaagacag attgacttcg cgaaaaaaag gctcgaaatt
     5461 aacgggcgtc gtcagcccct gaaaaaatgc ccgtccgccg gtttgttccg aaagtttttg
     5521 gagcgagccc tgcgcgttta aaatcagcgt cgtgctggcg cgcgcggtca ggctcgccga
     5581 agcgtaaaac gaataaaccg ccacgctttt gcgctgcgct ttggtaatcg cgcgttcgag
     5641 gtcgaggctg cccgtcggcg acgaatcgtc caacccgcgc gaagcatcca aaccatccga
     5701 gattaaaaga atcgcgcgcc tgccgattgg caaagcgtcg aaacgaccca gcgcttcttc
     5761 gacgctttga taaggactgc tcggcgcagc cgaagcaacg ccggcgataa ttcgcagcga
     5821 gcccgccgct ttttccaaat cttcggtaaa tttttgccga acctgaatcg aaccggcgcg
     5881 taaatacgcg accatcacgc gcgagccttt cggcagacgc ctgataaaat cggcgaggcg
     5941 tttgagctcc agattgacgc tcgaagaaac gtcgtcctga atcagaaccg ccagcgccat
     6001 cggcgcttcg ccgatgctgc ggattgagag aatcgtttgc tggtcgccgt cttctctgac
     6061 gatgatgttg ccggcttcaa taaattcctg cggaatctgt tgttttaatt cctctttcga
     6121 aaaaatcgaa atcggaatcg tcacggtgcg aactttttgt tgtttttttt ggttgttttc
     6181 gttttcgggc tgcgccaaac aaaaatttgc cgcaagcaaa gtcgaaacga aaacaacgaa
     6241 aaactttcga gcaatcattt tgaacatagg cgctttcagt ccttcaagct aataatatta
     6301 cattatcgtc aagccgttaa gttttttgat gcgatatatt tgaataaagt ttgtagagta
     6361 ggttagagta aaagccgaat gaacagttta gagtcgcaat attacgaggt gacgcgccgc
     6421 gatttgtcaa aagacaaggc gctgaaattc ggcgcgtggg tcgtgccgtt tgtgacggcg
     6481 attctgcccg cgcttgtttt ctttattctt ttcctattct ccagcgccac gccgacggcg
     6541 gcgatgtttt tctttctctc gctcgtctcg ctcgtcggcg gttttatttt cggtttgata
     6601 atcggcggcg gcttgatgtt ttatcgctcg cgctggctca aacagattcg cgagcgcatc
     6661 gccgtcgacg gcataaaagc caacgaagtc gatttttttc aacacgaatt aacttccgcc
     6721 gaaagaaaat cgctcaggga aatcgaaaat aaagaccgta tgcttgccga cgcttatcgc
     6781 gaaactctgg cttcgcgcct gaccgcgacg cgaattctga aaacgaccag gcaggagctt
     6841 cttttagtca ggcggcgcga gggcaaactg aaatacctga aaagcgaaaa ctccgaatcg
     6901 ctgcaaaaac agttgaaaga cgaccagacg aaactcgacg aaatcaaaac cgaagccgaa
     6961 cagatgcgcg tccaagccga aacgcgcctg caaatgatag aagccgcctc gcgccgcgga
     7021 acgaatttct cggatacgga gctcgcctta aaaaggctca cggcgcgctc agccgagctt
     7081 ccgctggcgc tcgaagccat aaaaatggaa gaagaaatcc gccgggaact ggaaaaagaa
     7141 gagacggaaa accagtgaaa agtaaaaaac taaaaagatg ctttgcacaa aaataaagga
     7201 aatcgagaaa aattccgatg gctaattgta aattctcaat tattaattct aaattactta
     7261 acaaaagata ccgaacatca atttagaatt tacaatttag aattt
//
LOCUS       DASWNE010000818         1760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_97537, whole genome shotgun sequence.
ACCESSION   DASWNE010000818 DASWNE010000000
VERSION     DASWNE010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97537"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..428)
                     /locus_tag="VGB68_16010"
     CDS             complement(123..428)
                     /locus_tag="VGB68_16010"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY0050798.1"
                     /translation="MSKTLLEVENLRTRFGAAKALDGVSFAVGEGEVLGIVGESGCGK
                     SVTALSIMRLIESPGEICGGRVIFRGGETEEIDLLKLSEKQLEKILGSRISLIFQDP"
     gene            complement(492..>1760)
                     /locus_tag="VGB68_16015"
     CDS             complement(492..>1760)
                     /locus_tag="VGB68_16015"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0050799.1"
                     /translation="TWFYYDIKGIQALKEGKGRADEVGVEALSERTVRIHSENGAIPH
                     LPALLAYQAAVPAPKHKAENNPEHWADTAEGFVSSVPMRLLRWEHNQRLEWDANPYYN
                     GPHKVGIQRLVQLLGAPAIGWLNSWLNKEIDYLPILQPQELAQVRNDAELEKYLHSFN
                     NFQIQYLSFDTLRPPFDNLKLRQALSHAIDREPFCRKVMMQTRVPAYSMLPPDFPAHN
                     PELKSVQDFNVEKARALLAEAGFPDGKDADGNQLVLEMFSNGRDVLLEYVKDQWERYL
                     DIGVNLQIVEGAVWSARRGRHEMPVYQGQYEYDFLDPANMLTRLWRSTSEKGSPRHAW
                     RNEKFDELVTQAAREIDEPKRIGLYKQAERILVEDVGGLFLTQTVTHQIWYPYLTGLE
                     PDKTGNVVFRYLDVSRFQMYIRNDVDEWRD"
BASE COUNT          393 a          499 c          440 g          428 t
ORIGIN      
        1 attccgacgc gccgcaaaag aaaaatcgcc tgctctctgg cttcttttcg cgataaaccg
       61 cgatgcacgc gcagagtttc cataatctga aacccgacgg acaaaaccgg attcagcgaa
      121 gtctaagggt cttgaaaaat cagcgagatg cgactgccga gaattttttc cagctgcttt
      181 tcggaaagct ttaataaatc tatttcttcc gtttcgccgc cgcgaaaaat cacccgcccg
      241 ccgcaaatct cgccgggcga ttcgataagc cgcataatcg aaagcgccgt cacgcttttg
      301 ccgcagccgg attcgccgac aatgcccaaa acctcgcctt ctccaacggc gaaagacacg
      361 ccgtcgaggg ctttcgccgc gccgaaacgc gtccgaagat tttcaacttc aagcaaagtt
      421 ttggacatag aattattgaa tcgccaatcg tcaatcgtgg agcttttcta ttttcgattg
      481 acgattggcg attaatcgcg ccattcgtcg acatcatttc ggatatacat ctgaaagcgc
      541 gaaacatcca gatagcgaaa aacgacattg ccggttttgt ccggctctaa gccggtcaga
      601 tacggatacc agatttgatg cgtgaccgtc tgcgtcagaa atagcccgcc gacgtcttcg
      661 accagaattc tttccgcctg tttgtaaaga ccgattcgtt tcggttcgtc gatttcgcgt
      721 gccgcctgcg tcaccagctc gtcaaatttc tcgttgcgcc acgcgtgtcg cggcgagcct
      781 ttttcgcttg tgctgcgcca gagccgcgtc agcatatttg ccgggtcgag aaaatcgtat
      841 tcgtattgac cctgataaac aggcatttcg tgccgccctc gcctagcgct ccagaccgcg
      901 ccttcgacga tttgcaggtt gacgccgatg tccagataac gttcccactg gtctttgaca
      961 tattccagca gaacgtcgcg cccgttggaa aacatttcca gaacaagctg attgccgtcg
     1021 gcgtcttttc catcagggaa accggcttcc gccaacagcg cccgtgcttt ttcgacgtta
     1081 aaatcctgta ccgatttcag ttcgggatta tgcgcgggaa agtccggcgg cagcatcgaa
     1141 tatgccggaa cgcgcgtctg catcattact ttccggcaaa aaggctcgcg gtcaatcgcg
     1201 tgcgacagcg cctggcgcag cttcagattg tcgaacggcg ggcgcaaggt gtcgaaagac
     1261 aaatattgaa tctgaaagtt gttgaaagaa tgcagatatt tttcgagttc cgcgtcgttg
     1321 cgcacctgcg caagctcctg cggctgcaaa atcggcaggt aatcgatttc cttgttcagc
     1381 cacgaattaa gccacccgat ggcgggcgcg ccgagaagct gcacgagccg ctgaatcccg
     1441 accttatgcg gtccgttata atacgggttc gcatcccatt cgagcctctg gttgtgttcc
     1501 cacctgagca ggcgcatcgg aacggacgaa acaaaacctt ccgccgtatc agcccagtgt
     1561 tccggattgt tttcggcttt gtgtttcggc gccggaacgg cggcttgata cgccagtaaa
     1621 gcgggcaggt gcggaatcgc gccgttttcg ctgtgaatgc gaacggttct ctcgcttaac
     1681 gcttcgacgc cgacttcgtc ggctcgtcct ttgccttctt ttaaagcctg aatgccttta
     1741 atgtcgtaat aaaaccacgt
//
LOCUS       DASWNE010000819         4360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_449205, whole genome shotgun sequence.
ACCESSION   DASWNE010000819 DASWNE010000000
VERSION     DASWNE010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449205"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VGB68_16020"
     CDS             <1..536
                     /locus_tag="VGB68_16020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050800.1"
                     /translation="SASVPVNYRWRVTPETLNVTSGAGTPSITVDTTGLGGQTITAEL
                     DVTDDVYGETCFQKNTVRTRVKPRIVVVPEKIRCDVFESTSFDDDKYRFDNCVIQLRN
                     RPDAQLYIIIYQGTDRLSRSRTTADLVRRRTMDYLVRTRGVDPRRIQVIMGGTRPRTS
                     IELWIVPPGASLPVPNE"
     gene            775..2172
                     /locus_tag="VGB68_16025"
     CDS             775..2172
                     /locus_tag="VGB68_16025"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF003211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEY0050801.1"
                     /translation="MSAPNIETIVSLAKRRGFVFPASEIYGGLGSSWDYGSLGVELAN
                     NIKRSWWRWLVHERDDIEGLDSSIIQNRLVWKYSGHETTFSDPLVDCRACKGRFRLDK
                     LQDVQCPKEPAVCAGSGKECDLTEAREFNLMFRTTVGAVSSDEDESALAYLRPETAQG
                     IFINFGNVLNTSRRKLPFGIAQIGKAFRNEVTPGNFIFRTREFEQMEMEYFCRPEDAP
                     HIHQEFIDAFQQWFYSLGISADNLRLYEQSAEELAHYAARTVDILYRFFPEREDEDKQ
                     FDELMGIANRKDFDLKAHSKKPENAEGKRLNPDSTDDLSYFDEATKQRFYPYVIEPAV
                     GVNRTLLAVLMDAYTEKTNDKNETRVILKLKPELAPIKVAVLPLAKNKPEIVALAKDL
                     KRQLLPAMRTVYDDTGGIGKLYARQDEIGTPFCVTVDHESLEDNAVTVRDRDTWEQER
                     IKVSELKNYLEKRLS"
     gene            2173..3021
                     /locus_tag="VGB68_16030"
     CDS             2173..3021
                     /locus_tag="VGB68_16030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050802.1"
                     /translation="MQKRFLILILLCLPLMALGQNSEYQGNYRALSLNFLTDDKFEAK
                     FEVAADGNILGKFFTGEQKIPVENDLIGKVDAKGKFEAQATRADGTIFSIKGELPKSA
                     GRVPIQFKKKLVVASKGKKQVGELETMGLLERLPSPPTVEAIFPDSGKTRLKIEYANS
                     IFDGVWENMPVKSIDETKPTGKTYRTLELKTENGDALRSLRFVLPLAENKTEWIVESG
                     KGWSVFYRENKGENKNLFFVTEAGSIHLAGEDERETVWQVKNLVMTNVRRTEKVKISG
                     YIHVAK"
     gene            3087..>4360
                     /locus_tag="VGB68_16035"
     CDS             3087..>4360
                     /locus_tag="VGB68_16035"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY0050803.1"
                     /translation="MAKLPEKIIRLAEIIRENGGRAMLVGGCVRDELMNVEPKDWDVE
                     VYNIEPPRLREILDSFGRVDAVGEAFTVYKIGQDLDVALPRRERKVGRGHKGFVVEGD
                     KDMTFAEAAKRRDFTINAILKDASTGEIVDVYGGRKDIENKILRVVSKETFAEDSLRV
                     LRAAQFAARLEFDIDAETVEICRRIDLTDLPKERIWGELEKILLQAEKPSTGLKRLYD
                     LGVVEQLFPELKSLVGVPQQPEWHPEGDVDVHTLMVADEARKLIDDLPYAKKAAVMLG
                     ALCHDFGKPPTTKFFDGKWRSHGHDEAGVEPTLSFLDKLGIFTLDGYDVRGQIVQLVR
                     YHLKPGEFYKAQPGDGAFRRLARRVEPDLLYRVAKADSLGRNPEWLPKEKWFKAEAQE
                     WFIEKVRELAIEKEAPKRILMGRHLIELGLKPS"
BASE COUNT         1142 a         1055 c         1215 g          948 t
ORIGIN      
        1 tttcggcgag cgtgccggta aattatcgct ggcgcgtaac gcccgagacg ctcaacgtca
       61 cgagcggagc cggaacgcct tcaatcacgg tcgatacgac cggactcggc ggtcaaacca
      121 tcaccgccga gcttgacgtg accgacgacg tttacggcga gacctgtttt cagaagaaca
      181 cggtcagaac gagagttaag ccgcggatag tagtagtacc ggaaaaaatt cgctgcgacg
      241 tgttcgaatc gacatcgttc gacgacgaca aatatcgctt tgacaactgc gtcatccagt
      301 tgcgcaatcg tccggacgct cagctttaca ttatcattta tcagggcacg gacagactca
      361 gccggtcgcg aaccacagcc gatttggtca ggagacgaac gatggattat ttagtcagga
      421 cgcgcggcgt cgacccgcgg cgaatacaag tcattatggg cggaactcgt ccgcgaacga
      481 gtatcgaact ctggatcgta ccgccgggcg catctctccc cgttccgaac gagtaatcgg
      541 aaacggaatg ttcaacggca aaaggacggc ttcggtcgtc cttttttatt ttgtcctttg
      601 tccgttgtcc tttgtccgtt gtaaaatcta cattttaaac tattaaatat cggaattgaa
      661 aaatcgttct tcgcagtttg cagtccgttg ccgctattca attgacaatg aacggctctg
      721 actgcgagcg gcggacaaaa tctaaaatcg caaatctaaa atcgaaaatt acttatgtca
      781 gcaccgaata tcgaaacgat tgtcagcctc gccaagcgca gaggctttgt ttttcccgct
      841 tcggaaatct acggcgggct cggaagcagt tgggattacg gctcgctcgg agtcgagctg
      901 gcgaataata tcaagcgcag ctggtggcgc tggctggttc acgagcgcga cgacatcgaa
      961 ggcttggatt cttcgattat ccagaatcgt ctggtctgga aatattccgg acacgagacg
     1021 actttttccg acccgctggt cgattgccgc gcctgcaaag gcagatttcg gctggataaa
     1081 ttgcaggacg tgcaatgccc gaaagagcct gcggtttgcg ccggaagcgg aaaggaatgc
     1141 gatttgaccg aggcgcgcga gtttaatctg atgtttcgga cgacggtcgg cgcggtttca
     1201 tccgacgaag acgaatcggc gctggcatac ttgagaccgg aaacggcgca gggaattttt
     1261 atcaatttcg gcaacgttct gaacacctcg cggcgcaagc tgccgttcgg catcgcgcaa
     1321 atcggcaagg cttttcgcaa cgaggtgacg cccggaaatt ttattttccg cacgcgcgag
     1381 tttgagcaga tggagatgga atatttctgc cgaccggaag acgcgccgca cattcatcag
     1441 gaatttatcg acgcttttca gcagtggttt tacagcctgg gaatttccgc cgacaatctc
     1501 cggctttacg agcagagcgc cgaagaactg gcgcattacg ccgcgcgaac ggtcgatatt
     1561 ttgtatcgct ttttcccgga acgcgaagac gaagacaagc agtttgacga attgatgggc
     1621 attgcgaatc gtaaggattt tgatttgaaa gcgcactcga aaaagccgga aaacgccgag
     1681 ggcaaacgtc tgaatccgga ttcgaccgac gatttatcgt atttcgacga agccacgaaa
     1741 cagcgttttt atccttatgt catcgaaccg gcggtcggcg tcaatcgcac tctgctcgcc
     1801 gttttgatgg acgcttacac ggaaaaaacg aacgacaaaa atgaaacgcg cgtgatttta
     1861 aaattaaaac ccgaattagc gccgataaaa gtcgccgtgc tgccgctggc gaaaaacaag
     1921 cccgaaatcg tcgcgctggc gaaagatttg aaacggcagc ttttgccggc gatgcgcacg
     1981 gtttacgacg acacgggcgg catcggaaaa ttatacgcgc ggcaggacga aatcgggacg
     2041 cctttctgcg tcacggtcga ccacgaatcg ctggaagaca acgccgtcac cgtgcgcgac
     2101 cgcgacacct gggaacagga aagaataaag gtttccgaac tgaaaaatta tctggagaaa
     2161 cgtttgagct agatgcaaaa aagatttcta attttaattt tattgtgtct gccgctgatg
     2221 gcgctcggac aaaattccga gtatcaaggc aattaccggg cgctgtcttt aaatttcctg
     2281 acggacgata aatttgaagc gaaatttgag gtcgccgccg acggcaatat tctcggaaaa
     2341 ttttttacgg gcgaacaaaa aattccggtc gagaatgatt tgattgggaa agtcgacgct
     2401 aaaggaaaat ttgaagcgca ggcgacgcgg gctgacggaa cgattttcag catcaagggc
     2461 gagctgccga aatccgccgg acgagtgccg attcaattta agaaaaaact cgtcgtcgct
     2521 tcaaagggaa aaaaacaggt cggcgagctc gaaacgatgg gcttgctcga acgcctgccg
     2581 tcgccgccga ccgtcgaagc gatatttccc gattcgggca aaactcgtct gaaaatcgaa
     2641 tacgccaact cgattttcga cggcgtttgg gaaaatatgc ccgttaaatc aatcgacgag
     2701 acgaaaccga cggggaaaac ttaccggacg ctggaattaa aaaccgaaaa cggcgacgcg
     2761 ctccggagtc ttcgattcgt cctgccgctg gcggagaaca aaaccgagtg gattgtcgaa
     2821 tcgggcaaag gctggtctgt tttttacagg gaaaacaaag gcgaaaacaa aaatctcttt
     2881 tttgtgaccg aagccggaag catccatctc gccggcgaag acgagcgcga aaccgtctgg
     2941 caggtgaaaa atctggttat gaccaacgtg cgaagaacgg aaaaagtgaa aatttcgggt
     3001 tacattcacg tcgcgaaata aaatttaaaa cgattcgggc gcgacaagcg gagagaattt
     3061 atcctgtttg cctgtccttt taatcgatgg cgaaacttcc ggaaaaaatc atcaggctcg
     3121 ccgagataat tcgggaaaac ggcggacgcg cgatgctcgt cggcggctgc gtgcgcgacg
     3181 aattgatgaa tgtagagccg aaggattggg acgtcgaagt ctataatatc gagccgccgc
     3241 ggctgcgtga aattctcgat tcttttggca gggtcgatgc cgtcggcgaa gcgttcaccg
     3301 tttataaaat cggtcaggat ttggacgtgg cgcttccgcg ccgcgaaaga aaagtcgggc
     3361 gcgggcataa aggtttcgtc gtcgaaggcg ataaagacat gacgtttgcc gaagcggcga
     3421 aacggcgtga ttttacgata aacgcgattc tcaaagatgc gtcgacgggc gaaatcgtcg
     3481 acgtttacgg cgggcgaaaa gatattgaaa acaaaatttt gcgcgtggtg tcaaaagaaa
     3541 cttttgcgga agattctctg cgcgtgctgc gtgcagcgca gttcgccgcg cggctcgagt
     3601 tcgatatcga tgccgaaacc gtcgaaatct gccgccgaat cgatttgacg gatttaccga
     3661 aggaaagaat ctggggcgag ctcgaaaaga ttttgctgca agccgaaaag ccttcaaccg
     3721 gtctgaaacg gctttacgat ttaggagtcg tcgagcagct tttccccgaa ctgaaatcgc
     3781 tcgtcggcgt tccgcagcag cccgaatggc atcccgaagg cgatgtcgac gtgcatactt
     3841 tgatggtcgc cgacgaagct cgaaaactga ttgacgattt gccgtatgcg aaaaaagcgg
     3901 cggtgatgct cggcgcgctc tgccacgatt tcggcaaacc gccgaccact aaattttttg
     3961 acggtaaatg gcgctcgcac ggtcacgatg aggcgggcgt cgagccgacg ctttcgtttc
     4021 tggacaagct cggaattttt acgctcgacg gctacgacgt acgcgggcaa atcgtccagc
     4081 tcgtgcgtta tcacctgaaa ccgggtgaat tttataaagc gcagcccggc gacggcgctt
     4141 ttcgacgcct ggcgcgaagg gtcgagccgg atttattgta tcgcgtggcg aaagccgatt
     4201 ctctcgggcg aaatcccgaa tggctgccga aggaaaaatg gtttaaggcg gaagcgcagg
     4261 aatggtttat cgaaaaagta cgcgaactgg caatcgaaaa agaagcaccg aaacggattt
     4321 tgatgggcag gcatcttatc gagctgggtt taaagccttc
//
LOCUS       DASWNE010000820         6822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_696889, whole genome shotgun sequence.
ACCESSION   DASWNE010000820 DASWNE010000000
VERSION     DASWNE010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6822
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696889"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VGB68_16040"
     CDS             <1..277
                     /locus_tag="VGB68_16040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050804.1"
                     /translation="VFCTNRSGRMECSFSFRESPTGEKKMSAYIQTGSSANSVEELAK
                     GYKREDVKIRDNAGNVINLADKVKVTGELNTTGDPNICFINVTKIEK"
     gene            277..834
                     /locus_tag="VGB68_16045"
     CDS             277..834
                     /locus_tag="VGB68_16045"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEY0050805.1"
                     /translation="MTRCDWAKNHLAIRYHDEEWGVPAHDDRRLFEFLILEGAQAGLS
                     WDTILRKRENYRAAFDAFQAEKIALYDAKKVAELLRDEGIVRNRLKIASTVRNAREFL
                     KIKDEFGSFDSYIWRFVGGAPIVNAWKNVSEVPAKTAISDAMSKDLKKRGFNFVGSTI
                     MYAFMQACGLANDHLVSCFRYEETI"
     gene            831..1373
                     /locus_tag="VGB68_16050"
     CDS             831..1373
                     /locus_tag="VGB68_16050"
                     /inference="COORDINATES: protein motif:HMM:NF015638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HEY0050806.1"
                     /translation="MNLPSFKYHPNPVETGSIEASENVCKCCEQVRGFIYTGPVYAAG
                     EFIDAICPWCIDDGTAHKKFDAQFTDSAGVGGYGEWGKVSDEIVEEVAYRTPGFSGWQ
                     QEMWFTHCGDAAEFLGVVGKEELEQLGTKAILAIKTEIGFEGEEWEDYFQYLDKNEGP
                     TAYIFKCRHCGEFGGYSDIH"
     gene            1351..1857
                     /locus_tag="VGB68_16055"
     CDS             1351..1857
                     /locus_tag="VGB68_16055"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEY0050807.1"
                     /translation="MVIPISIESMNYPVTPAIRVLREKKIFFTPHVFEYVEKGGTRHS
                     ARELGVDEHAVVKTLIFETNEKKPLIVLMHGDLQVSAKNLARHLGVKSAAPAAPEKAS
                     KLTGYMVGGTSPFGVKTRMPVYAEKTIFGLERIYINGGKRGFLIAIEPRVLRDVLQVE
                     EVEVAIKD"
     gene            1895..2446
                     /locus_tag="VGB68_16060"
     CDS             1895..2446
                     /locus_tag="VGB68_16060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050808.1"
                     /translation="MMLIDEFLPVYDFDEKHEINIRASAEKVYAALDSFDFNDSAVIR
                     WLFWLRGLASGNGGASSESSNLRDMTKSNFIILGEKPNEEIVLGLAGKFWALSGDLQK
                     LKAEEFSAFEKEGYAKAVWNFTLSEAGEAETCLKTETRVRCLGAAGHESFRFYWMFVK
                     PFSGWIRQEMLRLVKRKAEEAAR"
     gene            2453..2791
                     /locus_tag="VGB68_16065"
     CDS             2453..2791
                     /locus_tag="VGB68_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050809.1"
                     /translation="MSLYTRWACFDCRKSFAKDASETPRKCPECAGAMIDMGAYFEPP
                     RRLSKKRWEVMKVLADNGYKFNDRDSRVYIQNRILQVKNPRVADVIERIEAERRRKKE
                     SAADNTGKER"
     gene            2873..4159
                     /locus_tag="VGB68_16070"
     CDS             2873..4159
                     /locus_tag="VGB68_16070"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0050810.1"
                     /translation="MKTINVAQKIIISGLLFLCFAAAAGAQVTAIRAGRIIDPETGAV
                     SNNQIILVEGQNIKAIGANVQIPAGAIVVDLSKQVVLPGLFDAHTHLCMNMRHQRDAG
                     SYYITTLLDSNGMRAIQGVANARSMLEYGFTTVRDVGNAGNYADTDLRRAIDQETVPG
                     PTIINAGRIIAPYGGQFQMQPDKPNLGNPEYFYADTRDELKKAVRENIHYGARVIKLV
                     VDDQRYIYSVDDIKFVIEEARLAGMKVAAHCWTQQGARNAAEAGVASIEHGVEIDDAT
                     LEIARRNNVALVPTPFTETDARMRGEPEGGNKENDARWFADPVKRANAKGVTLVFGPD
                     VIFTTKEYPRGRLSIETIDNWVAAGIPPRVILQALTVNAAKLLGVEKTRGYLKAGMRA
                     DIIAVSDNPVEKIETLKNVGFVMKNGKVFKQNSVVK"
     gene            4466..5644
                     /locus_tag="VGB68_16075"
     CDS             4466..5644
                     /locus_tag="VGB68_16075"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY0050811.1"
                     /translation="MKEAVIVSAVRTAVGKAPKGTLKNTRPDEMGAVAIKEAVRQVPN
                     LDASAIDDVIMGCAFPEAEQGQNVARIAAIRAGLPVEVSAMTVNRYCSSGLQTIAIAA
                     DRIKTGGAEIIVAGGLETMSMIPMGGHSVRPNPVLVDTYPDYYLNMGLTAEALAQKYE
                     ISREAADEFSLESHRKAAAAIKAGKFKDEIVPLSVRIDETGEKGRVLRREIAFDTDEG
                     VRFDATMEGLAKLKPAFHAKGTVTAGNASQMSDGAAAVVIMSAEKAAELGAKPLARFV
                     AYATSGCLPEEMGIGPVYAIPKVLKMAGLTLDDIEVIELNEAFAAQSLAVIKALEMNP
                     EKINVNGGAVALGHPLGCTGAKLTATILREMERRGARYGMVTMCVGGGMGAAGILERI
                     "
     gene            5760..6005
                     /locus_tag="VGB68_16080"
     CDS             5760..6005
                     /locus_tag="VGB68_16080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050812.1"
                     /translation="MSEKRRIQGLDKAPLSMKLLYLASQKMVGKVVEPLKVQAHRPSI
                     AWFGNLLGLVIEKSGKVEARIHVLAQLRAAQIVECPF"
     gene            6006..6302
                     /locus_tag="VGB68_16085"
     CDS             6006..6302
                     /locus_tag="VGB68_16085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY0050813.1"
                     /translation="MDIGSAVGRQKDVVTDEELREVPNWQHSKILGERERAAIEYAEE
                     MSKTPVRVSDELFERLKKHFSDEQIVELSASIAYENYRARFNHALDIKSDNLYR"
     gene            6317..>6822
                     /locus_tag="VGB68_16090"
     CDS             6317..>6822
                     /locus_tag="VGB68_16090"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0050814.1"
                     /translation="MDATAQKKEITGGSFLIEERSASEVFTPEDLTEEQRMIGETTRE
                     FVDTEVRPNIPEMEKHNWRLARDLVAKGGELGLLGATIPEEYGGLALDQTSGVVIAEM
                     MGRAGGFGTTFGAQTSIGLLPILYFGSDELKEKWIPRIVSGEIVTAYCLSEAGSGSDA
                     LGAKCSARL"
BASE COUNT         1785 a         1556 c         2022 g         1459 t
ORIGIN      
        1 agttttttgc acgaaccgca gcggtcggat ggaatgcagc tttagcttca gggaaagccc
       61 gacgggcgaa aaaaagatga gcgcgtatat ccaaaccgga agctcggcaa acagcgtcga
      121 ggaactggca aagggctaca agcgcgaaga cgtgaaaatc cgcgacaatg ccggaaacgt
      181 tatcaatctc gccgacaaag ttaaagtgac gggcgaattg aacacgacgg gcgacccgaa
      241 catttgtttt ataaacgtga cgaaaattga aaaataatga cgcgctgcga ttgggcgaaa
      301 aaccatttgg cgattcgtta tcacgacgag gaatggggcg tgccggcgca cgacgaccgg
      361 cgtttgttcg agtttttgat tctggaaggc gcgcaggcgg gcttgagctg ggacacgatt
      421 ttgcggaaac gcgaaaatta ccgcgccgct ttcgacgcgt ttcaagcgga aaaaattgcg
      481 ctgtacgacg cgaaaaaagt cgcggagctt ttgcgggatg aaggaattgt ccgcaatcgt
      541 ctgaaaatag cctcgaccgt ccggaacgcg cgggagtttt tgaaaattaa agacgagttc
      601 ggctctttcg attcgtatat ctggcggttt gtcggcggcg cgccaatcgt caacgcctgg
      661 aaaaacgtgt ctgaagttcc ggcgaaaacc gcaatctcgg acgcgatgag caaagatttg
      721 aaaaagcgcg gctttaactt tgtcggctcg acgataatgt atgcgtttat gcaggcttgc
      781 gggctggcga acgaccatct ggtttcgtgt tttcggtatg aggaaacaat atgaatttgc
      841 cgagttttaa atatcatcct aatccggtcg aaacaggcag catcgaagct tcggaaaacg
      901 tttgcaagtg ttgcgaacaa gtcagaggat ttatttatac aggtcctgtt tatgctgcag
      961 gagagtttat tgacgcgatt tgtccctggt gtatcgacga cggaacggct cacaaaaaat
     1021 ttgacgcgca attcacggat tcagccggag taggcggtta cggcgaatgg ggaaaagttt
     1081 ccgatgagat tgtcgaggaa gtcgcctatc gaacgcccgg ctttagcggc tggcagcagg
     1141 aaatgtggtt tacgcattgc ggcgacgccg cagagttttt gggcgttgtc ggaaaagaag
     1201 agcttgagca attggggacg aaagcgattt tagctattaa gactgaaatc ggatttgaag
     1261 gcgaagaatg ggaagattat tttcagtatc ttgataaaaa tgaaggtcct accgcataca
     1321 tttttaaatg tcggcattgc ggagaattcg gtggttattc cgatatccat tgaaagtatg
     1381 aattacccgg tcacgcccgc gattcgcgtt ttgcgcgaga agaaaatctt tttcacgccg
     1441 cacgttttcg aatacgtcga aaaaggcggg acgcggcatt cggcgcggga attgggcgtc
     1501 gacgagcacg cggtggtgaa aactctgata tttgaaacga acgagaaaaa gcctttaatc
     1561 gtgttgatgc acggcgattt acaggtttcc gcgaaaaatc tggcgcggca tttaggcgtt
     1621 aaatcggctg cgcccgccgc gcccgaaaaa gcgtctaaat taacgggtta tatggtcggc
     1681 ggaacgtcgc ctttcggcgt gaaaacacgg atgccggttt acgccgagaa aacgattttc
     1741 gggttagagc ggatttacat caacggcggc aaacgcggat ttttgattgc gatcgagccg
     1801 agggttttgc gcgatgtttt acaggtcgaa gaagtcgagg tcgccattaa agattaatga
     1861 aaaagagctt tacagcttct gattgagaat aaaaatgatg ttgattgacg aatttttgcc
     1921 ggtttatgat ttcgacgaaa agcacgaaat aaacattcgc gcttcggctg aaaaagttta
     1981 tgcggcgctc gattcgtttg attttaatga ctcggctgtt attcgctggc tgttttggct
     2041 gcgaggtttg gcatccggaa acggcggcgc gagttcggaa tcttcaaatc tgcgcgatat
     2101 gacgaaatcg aattttatca ttttgggaga aaagccgaac gaggaaatcg tgctcggact
     2161 cgccggaaaa ttctgggctt tgtcgggcga tctgcaaaag ctgaaagcgg aggaattttc
     2221 cgcttttgaa aaagaaggtt acgccaaagc cgtctggaat tttactttat cggaagccgg
     2281 tgaagcggaa acctgcttga aaacggaaac cagagtgcgc tgtctgggcg cggctggtca
     2341 cgaaagcttt agattttact ggatgttcgt caagcctttt agcggttgga ttcgtcagga
     2401 gatgctgcgg ctggtgaaac ggaaagccga ggaagcggct cggtaaattt taatgagttt
     2461 atacacgcgc tgggcatgtt tcgattgccg gaaaagcttc gccaaagatg cgagcgagac
     2521 gccgcgaaaa tgtcccgagt gcgccggagc gatgattgat atgggcgcgt atttcgagcc
     2581 gccgcgccgt ctaagcaaaa agcgctggga ggtgatgaaa gtgctggctg ataacggcta
     2641 caaatttaac gacagggaca gccgggttta cattcaaaac cggattttgc aggtgaaaaa
     2701 tccgcgcgtg gcggatgtga ttgagagaat tgaagcggaa agacgacgaa agaaggaatc
     2761 agccgcagat aacacgggga aagaacgatg aaaccgccga tagacgccga tagacgccga
     2821 ttggaaacgg atttattttg attttattcg ggtaatttgc cgctaaaaaa ttatgaaaac
     2881 tatcaacgtg gcgcaaaaaa taattatctc cggtttgctt tttctgtgtt ttgctgcggc
     2941 ggcgggcgcg caggtgacgg cgattcgcgc gggcagaata atcgacccgg aaacaggtgc
     3001 ggtttccaac aatcagatta ttctggtcga agggcaaaac atcaaagcca tcggcgcaaa
     3061 cgtgcagatt ccggctggcg cgattgttgt cgatttgtca aaacaggtgg ttctgcccgg
     3121 tttgtttgac gcgcacacgc atctttgtat gaatatgcgg caccagcgcg atgcgggcag
     3181 ttattacatt acgacgctgc tcgattcgaa cggaatgcgc gcgattcagg gcgtggcgaa
     3241 cgcgcgctcg atgctcgaat acggtttcac gaccgtgcgc gacgtcggca atgccggaaa
     3301 ctacgccgac acggatttgc gccgcgccat cgaccaggaa accgttccgg gtccgaccat
     3361 cattaacgcc ggacgcatta tcgcgcccta cggcgggcag tttcagatgc agccggacaa
     3421 accgaatctg ggaaatcccg aatattttta cgccgacacg cgcgacgagc tgaaaaaagc
     3481 cgttcgcgag aatattcatt acggcgcgcg cgtgataaaa ctcgtcgtcg acgaccagcg
     3541 ttatatttat tcggtcgacg acatcaaatt cgtgattgaa gaagcgcggc tcgccggtat
     3601 gaaagtcgcg gcgcactgct ggacgcagca aggcgcgcgc aatgcggcgg aagcgggcgt
     3661 cgcatccatc gaacacggcg tcgagataga cgatgcgacg ctggaaatcg ccagacgcaa
     3721 caacgtcgcg ctcgtcccga cgccgtttac cgaaaccgac gcgcggatgc gcggcgaacc
     3781 ggagggcggc aataaagaga acgacgcgcg ctggtttgcc gatcccgtca aacgcgctaa
     3841 cgcgaaaggc gtgacgctcg ttttcggtcc cgacgtgatt tttacgacga aagaataccc
     3901 gcgcggacgg ctctcgattg aaaccattga taactgggtc gccgccggaa ttccgccgcg
     3961 cgtgattttg caggctttga cggtgaacgc cgcgaaattg ctcggcgtcg aaaaaactcg
     4021 cggctatctg aaagccggaa tgcgagccga cattatcgcc gtcagcgata atccggtcga
     4081 gaaaatcgag acgctcaaaa acgtcggttt cgtgatgaaa aacggcaagg tttttaaaca
     4141 aaattcagtc gttaaataga aggaaaaggt atgtcgcgcg taaaaattca aatttatcac
     4201 gaaaacaatg cggcgcgtga gagcaaaccg gctcggacga tttggattga aagaaattcg
     4261 gataaaaagt tgacgggtaa gcaggcgcgg caaattttag ccgaagaatt tcctcggttt
     4321 gtaaagagtt atgggatttt attaacgaaa accagcgaag gctggcgagc gagccggaca
     4381 ataagaccga ctgcgaaatg cgattatcat tacatttggg aaagggcaat tattgcggaa
     4441 gaagagtcac agtaaaggaa aaattatgaa agaagcagtt atcgtttcag ccgtgcgcac
     4501 ggctgtcgga aaagcgccga aaggaacttt gaaaaacact cgaccggacg agatgggcgc
     4561 ggtcgccatt aaagaagcgg tcagacaagt gccgaacctc gacgcgtccg cgattgacga
     4621 cgtgattatg ggctgcgcgt ttcccgaagc cgagcagggg cagaacgtcg cgcgcatcgc
     4681 cgcgattcga gccggtctgc cggtcgaggt cagcgcgatg acggtcaatc gttattgctc
     4741 gtcgggcttg cagacgattg cgattgcggc ggacagaatc aaaacgggcg gcgcggaaat
     4801 tatcgtcgcg ggcggcttgg aaacgatgtc gatgattccg atgggcggtc acagcgtgcg
     4861 cccgaatccg gtgctggtcg acacgtatcc cgattattat ctgaatatgg gcttgaccgc
     4921 cgaggcgctg gcgcagaaat acgaaatctc gcgcgaagcg gcggacgaat tttcgctcga
     4981 atcgcaccgg aaagcggcgg cggcaatcaa agccggaaag tttaaggacg aaatcgtgcc
     5041 tttaagcgtc cgcattgacg aaaccggcga aaaaggtcgc gttctgcgcc gcgaaatcgc
     5101 tttcgacacg gacgaaggcg tgcgttttga tgcgacgatg gaaggcttgg cgaaactcaa
     5161 accggcgttt cacgcaaaag gcacggtcac ggcgggcaac gcctcgcaga tgtcggacgg
     5221 cgcggcggcg gtcgtcatta tgtcggcgga aaaagccgcg gaattaggcg cgaagccgct
     5281 cgccagattt gtcgcctacg cgacttccgg ctgcctgccc gaagaaatgg gcatcggtcc
     5341 ggtttacgcg attccgaaag ttttgaaaat ggctggcttg acgctcgacg acatcgaagt
     5401 catagagctt aacgaagcct tcgccgcgca atctctggcg gttatcaaag cgctggaaat
     5461 gaaccccgaa aaaatcaacg tcaacggcgg cgcggtcgcg ctcgggcatc ctctgggctg
     5521 caccggcgcg aagctgacgg ctactatctt acgcgaaatg gagcgccgcg gcgcgcgtta
     5581 cggaatggta acgatgtgcg tcggcggcgg tatgggcgcg gcgggaattc tggaaagaat
     5641 ttagtttgag ccacagaggc acggaaacac agagattttt aagaatatga aaccacagat
     5701 tacacagata aacatagata gttttttgta tttctctgtg tttctatggt gaaaaaatta
     5761 tgagcgaaaa acgacgaata cagggcttgg ataaagcgcc gctttcgatg aagctgcttt
     5821 atctggcttc ccaaaaaatg gtcggcaagg tggtcgagcc tttgaaagtt caggcgcatc
     5881 gtccttcgat tgcgtggttc ggcaatctgc tgggactggt gattgaaaaa tccggcaagg
     5941 tcgaagcgcg gattcacgtc ctggcgcagc ttcgcgcggc gcaaatcgtc gaatgtcctt
     6001 tctgaatgga catcggctct gcggtcggca ggcaaaaaga cgttgtcacg gacgaggaat
     6061 tgcgcgaagt tccgaactgg cagcacagta aaattttagg cgagcgcgag cgcgcggcga
     6121 tcgaatacgc cgaagaaatg tcgaaaacgc ccgtgcgcgt atccgacgaa ttgtttgaaa
     6181 gattgaaaaa acatttcagc gatgagcaaa tcgtcgagct ttcggcgagc atcgcttacg
     6241 aaaactaccg ggcgagattt aatcacgcgc tggatataaa gagcgataat ttatacaggt
     6301 aatcaggaga aaatttatgg acgcaacagc acagaaaaaa gaaataaccg gcggctcgtt
     6361 tttgattgag gaaagaagcg cgagcgaggt tttcacgccc gaagatttga ccgaagagca
     6421 aagaatgatt ggcgagacga cgcgcgaatt cgtcgatacg gaagttcgac cgaacattcc
     6481 ggaaatggaa aagcacaact ggcggctcgc gcgcgatttg gtcgccaagg gcggcgaatt
     6541 gggcttgctc ggcgcgacga ttcccgaaga atacggcggg ctggcgctcg accagacttc
     6601 cggcgtggtg attgccgaaa tgatgggacg cgccggcggc ttcggcacga ctttcggcgc
     6661 gcagacctcc atcgggcttt tgccgattct ttatttcggc tcggacgaac tgaaggaaaa
     6721 atggattccg cgcatcgttt ccggcgaaat cgtcacggct tattgcctgt cggaagccgg
     6781 ctccggctcg gacgctctgg gcgcaaaatg cagcgcgcgg ct
//
LOCUS       DASWNE010000821         2131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_131796, whole genome shotgun sequence.
ACCESSION   DASWNE010000821 DASWNE010000000
VERSION     DASWNE010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131796"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            421..1536
                     /locus_tag="VGB68_16095"
     CDS             421..1536
                     /locus_tag="VGB68_16095"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY0050815.1"
                     /translation="MNLKRKIIVALFCLAALPLAATAQNTKGLPQSKLEKIEAAIAAW
                     MAQHKAPALSVAIVTNNQLSWSKGYGLADVENSVSAKADTAYRLASITKVLTAVAVMQ
                     LVEKGKIDLNAPVNKYCAAYTEKQSLKDAPDKQFSITVRQLLVHFSGVRHNKPDEVFS
                     TRHFSSINEAVGTFKDDPLVIEPEAKYSYSTPGYTLLGCAIEGASGTNYMDYLRENIF
                     KPAGMTRTFADDVYTIIPNRARGYAKTQAGEIRNAPLHDTSIKIPGGGTVSTAEDMAR
                     FAAALNAGLLVKRETLEQMWTKPKTRDGKESNYAFGFLVNTQGGQRKVFNDGSQAGTR
                     TYLFMIPEQKFAVALMTNLERAWCEELVPKIIEALKE"
     gene            1785..>2131
                     /locus_tag="VGB68_16100"
     CDS             1785..>2131
                     /locus_tag="VGB68_16100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050816.1"
                     /translation="MKVYSGSTPFFRYALLAVVLCLCGSSAFAQEKSEKRGNFCENYN
                     YSSGDDKISHREVRESTLSAGSLVNVDARRNGGIRVRGENRSDVLVRACVQTRAATEE
                     EARSIARGIRIETG"
BASE COUNT          588 a          518 c          514 g          511 t
ORIGIN      
        1 ttcataaaac tctatttaag gttgataaaa ctctatttaa ggttcataaa actctattta
       61 aggttgataa aactctattt aaggctcgct aaactcgatt tatagttcgt caaactctgt
      121 tcgaggctca taaaacttta attaagcttc gtcaaactcg attcagggct gacgaaactt
      181 gatttaatga acgtaaaact cgattcgctg ttcatcgaac tcagcgcgcg gtatgcaaaa
      241 ctcgatgcac cgcgccatat attcgattca tcgttcaatg ttattcgaat tcatcattca
      301 atgttattcg aattcattgt tcaatgtatt cgattcattg tattgtgtat tagatttaca
      361 aacgcttgaa tcgagtgtcg cgttaaggaa atttcgcagc cgtttctgga gactaaaccc
      421 gtgaatttaa aacgtaaaat aattgtcgct ttgttttgtt tggcggctct gccgctcgcg
      481 gctacggcgc aaaacaccaa aggcttgccg caatcgaagc tggaaaaaat cgaagcggcg
      541 attgcggctt ggatggcgca gcacaaagcg cccgcgctgt cggtcgccat cgtcacgaac
      601 aatcaattga gctggtcgaa aggctacggg ctggcggacg tggaaaattc cgtttccgcg
      661 aaagccgata ccgcataccg tctggcttcg ataaccaaag tcctgaccgc tgttgccgtt
      721 atgcagctgg tcgaaaaagg aaaaatcgat ttaaacgcgc cggtcaataa atactgcgcc
      781 gcttacacgg aaaagcagtc gctgaaagac gcgcccgaca aacaattttc gataaccgtc
      841 cggcaattgc tcgtgcattt cagcggagtg cgccacaaca agccggacga agttttctcg
      901 accagacatt tcagcagcat taacgaagcg gtcggaactt tcaaagacga ccctttggtt
      961 atcgaacccg aagcgaaata ttcatattcg acgcccggct acacgctgct cggctgcgca
     1021 atcgaaggcg cttccggcac aaattatatg gattacctgc gcgaaaacat ttttaagccc
     1081 gccgggatga cgcgtacttt tgccgacgac gtctacacga ttatcccgaa ccgcgcccgc
     1141 ggttacgcga aaacgcaggc gggcgaaatc agaaacgcgc cgcttcacga cacgagcatc
     1201 aaaattcccg gcggcggaac cgtctcgacc gccgaagata tggcgcgttt tgccgccgcg
     1261 ctcaacgccg gtttgctcgt caaacgagaa acgctcgaac aaatgtggac gaaaccgaaa
     1321 acgcgcgacg ggaaggaatc gaattatgct ttcggatttc tggtcaacac gcaaggcgga
     1381 cagcgaaaag ttttcaacga cggcagccaa gccggaacgc gcacgtatct gtttatgatt
     1441 ccggagcaga aattcgccgt cgctttaatg accaatctgg aaagagcctg gtgcgaggaa
     1501 ctcgtgccga aaattatcga ggcgctgaaa gagtagaaag gcaaaagtaa aaactttccc
     1561 ttttgctttt ttgggaaaga caaattcggt tttccgaaag taattcgaat taaacgccga
     1621 caaatccggc tcgaagaaaa ggactttact cgatttataa atcgggtaaa gtcttatttt
     1681 tttaagttct tccaatttag tgtcataaac cggcgacgcg cggtgtttac ctgatagaaa
     1741 acgcaatccg cgaaatttaa atactaatca ggagagaatt taaaatgaaa gtttattcag
     1801 gcagcacgcc atttttccgt tatgctttgc tcgcagtcgt tctttgtttg tgcggttctt
     1861 ccgcttttgc gcaggaaaag agcgaaaagc gcggaaattt ctgcgaaaat tataattatt
     1921 cgagcggcga cgataaaatc tcgcacaggg aagttcgcga atctactttg tcggcgggaa
     1981 gtctggtcaa tgtcgatgcc cggcgcaacg gcggcatccg cgtccgcggc gaaaaccgca
     2041 gcgacgtgct ggttcgcgcc tgtgtccaga cgcgcgccgc gaccgaagaa gaagcgcgct
     2101 caatcgctcg cggcatccgc atcgaaaccg g
//
LOCUS       DASWNE010000822         2413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_97699, whole genome shotgun sequence.
ACCESSION   DASWNE010000822 DASWNE010000000
VERSION     DASWNE010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97699"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /gene="dcd"
                     /locus_tag="VGB68_16105"
     CDS             <1..618
                     /gene="dcd"
                     /locus_tag="VGB68_16105"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEY0050817.1"
                     /translation="SLSFFYEHFMTIKSDVWLRRMAEEQKMIDPFLPALVRETNGSRI
                     ISAGASSYGYDMRLADDGFRIFSSVYGLEIDPKHFDENSLIEPMLYTAEDGSKYYLLP
                     PHHYGLGVTVETFQMPRNVTGIALGKSTYARAGLLVNTTPLEAGWTGRLVVEIANLAN
                     LPLRVYVNEGIGQILFFESDENCAVSYSDRGGKYQGQTGLTYAKV"
     gene            complement(726..>2413)
                     /locus_tag="VGB68_16110"
     CDS             complement(726..>2413)
                     /locus_tag="VGB68_16110"
                     /inference="COORDINATES: protein motif:HMM:NF018259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HEY0050818.1"
                     /translation="WRVQTWDLKMPEVSPRAILYNLPPVAIGTADAESLTGYISRLAQ
                     EHYLSPPVLLKNCVREIPKLPKLLLQDSISSVFARSLNGFSENAAAIVEILQNATFRD
                     DILYTTLLPWKGKLSNQKLLKKQMAWCPVCYEEQREEGIVYDKLIWTLEKIKACQVHE
                     LPLLDSCPHCSKKLKVLSGKSRPGYCSRCLRWLGSNLISFDDLRGFRNDKDKKIEVWR
                     AVKAGEFLAKSSPFFMSEDHPVFIENLNEFIQTFTVGNINDFAHKTRMWHMSIRRLLK
                     GEVLPTLEMLFNICFPLDISPIELFQQNTEFKGEETGSLSNENKPSSKDEMKICLDNL
                     LLEYPPPSANEVSSRTVWRIARWQRNFPEEYKAIVKRYTRHVKENIPKLTDEEIEKIL
                     LQATKENPAPSLQSVFRRIGCRNTGYRYYRRFPQLCEEISNRYKKLNDKTFDVKKARK
                     IMKSALEENPPPSFSEIARRIKCNRETLDKKLPELSKSLHERYESYLEENRKNNHREL
                     YNAIKNAIIKLQMKQSSVTENSVRKDMPRKWNDKTFKETYRKVKQDLEITTDS"
BASE COUNT          605 a          539 c          511 g          758 t
ORIGIN      
        1 tcgctttcct ttttttacga acattttatg acgattaaat ccgacgtttg gcttcgccga
       61 atggctgaag aacagaaaat gattgacccg tttctgcccg cgctggtcag ggaaacgaac
      121 ggcagccgca tcatcagcgc cggcgcttcc agctacggtt acgatatgcg cctggcagac
      181 gacggctttc gtattttttc atccgtctac gggctggaga ttgacccgaa gcattttgac
      241 gaaaattcat tgattgaacc gatgctttac acagctgaag acggctcgaa atattatctt
      301 ctgccgccgc atcattacgg cttgggcgtg acggtcgaaa cttttcaaat gccgcgcaac
      361 gtgacgggca tcgcgctcgg aaaatccacc tacgcgcgcg ccggcttgct ggtcaatacg
      421 acgccgctcg aagccggatg gacgggacgc ctggtcgtcg agattgcaaa tctggcaaat
      481 ttgccattgc gcgtttatgt caacgaaggc atcggtcaga ttctcttttt tgaatcagac
      541 gaaaactgcg ccgtttctta ttcagacaga ggcggaaaat accaaggtca gacaggttta
      601 acctacgcga aagtttaaga aattatgcta ttgttttttt aagcatcgtt tgtccgaaac
      661 ggacattcga tgcttaaata cactcgcgga cattcgatgc ttaaataatc cggcaaaata
      721 aaaaactagc tgtcggtggt tatctcaagg tcttgtttta cctttcgata agtttccttg
      781 aacgttttgt cgttccactt tctcggcata tcttttctga cggaattttc ggtgacggat
      841 gattgcttca tttgcagctt tataattgca ttttttatcg cgttataaag ctctcgatga
      901 ttgttctttc tattttcctc cagataactc tcatatcttt cgtgtaaaga ttttgataac
      961 tccggcagct tcttatccaa agtttcgcga ttgcatttta ttcttctggc gatttccgaa
     1021 aatgagggcg gcggattttc ttccaatgcg gatttcatta tctttctcgc ctttttaaca
     1081 tcaaaagtct tgtcgttcaa ttttttataa cggttggaaa tttcttcgca taattgggga
     1141 aatctacggt agtatcggta acctgtattt cggcaaccaa ttcttctgaa cacgctttgc
     1201 agcgacggcg cgggattttc tttggtcgcc tgaagcaata ttttttcaat ttcctcatca
     1261 gtcaatttcg ggatattttc tttaacgtgc cgggtatatc ttttgacaat tgctttgtat
     1321 tcttcgggaa agtttctctg ccaacgtgca attctccaga cggttcggct cgaaacttca
     1381 tttgccgatg gcggtggata ttccagcaaa agattatcca gacaaatttt catttcatct
     1441 tttgaacttg gtttgttttc gttactgagc gagccggttt cttcgccttt aaattcggta
     1501 ttttgttgaa atagttcaat aggtgaaatg tcgagcggaa aacaaatatt aaataacatt
     1561 tccaaggtcg gcaacacttc gcctttgagc aatcttctga tcgacatgtg ccacatccgt
     1621 gttttatgag cgaaatcatt aatgtttccc acagtaaatg tttgaataaa ctcgtttaga
     1681 ttttcgataa aaacaggatg atcttcagac ataaaaaaag gagaactttt tgctaaaaat
     1741 tctcctgctt tcactgctct ccaaacttct atctttttat ccttgtcgtt ccgaaagcct
     1801 ctcaagtcgt cgaaactaat aaggtttgaa ccgagccacc tcaaacacct ggaacaatat
     1861 ccggggcggg atttgcctga tagaaccttt aatttcttac tgcaatgcgg acacgaatcg
     1921 agcaaaggca gttcgtgaac ctgacacgcc tttattttct ctagtgtcca aatcagcttg
     1981 tcatagacaa ttccttcttc tctttgctct tcgtaacaga cggggcacca agccatctgt
     2041 ttttttaata atttttgatt ggaaagcttt cctttccaag gcagaagagt agtgtataaa
     2101 atgtcgtccc gaaaagtggc gttttgcaaa atttcgacga tagctgccgc attttcgctg
     2161 aacccgttca aacttctcgc aaaaacggag ctgatactgt cttgcagtag gagtttcgga
     2221 agtttcggaa tttcccggac gcagtttttc agcaataccg gcggcgaaag ataatgttcc
     2281 tgcgctaatc gggatatata acccgtaaga ctttcggcat cggcagttcc gatagcgacc
     2341 ggcggcagat tataaagaat tgctctgggc gaaacttccg gcatttttaa gtcccaggtt
     2401 tggacacgcc aga
//
LOCUS       DASWNE010000823         1518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_131862, whole genome shotgun sequence.
ACCESSION   DASWNE010000823 DASWNE010000000
VERSION     DASWNE010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131862"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VGB68_16115"
     CDS             complement(<1..516)
                     /locus_tag="VGB68_16115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050819.1"
                     /translation="MKNKLNLSLRVLAIFAFAISFTTAINAQATRTWVSGVGDDVNPC
                     SRTAPCKTFAGAISKTAANGEINALDPGGFGTINITKSMVIDGVNPGMASILAAGTTG
                     VIVNALSTDTVILRNLTINGAGTGVNGIRILNAKAVYIENCVIFNFVGSSGTTGRGIT
                     DERAHAGGFLYI"
     gene            complement(998..>1518)
                     /locus_tag="VGB68_16120"
     CDS             complement(998..>1518)
                     /locus_tag="VGB68_16120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HEY0050820.1"
                     /translation="KELGVAHIPEDRHNRGLLLDSDLEENSILGVHYRPPLTAGGFLN
                     ASAIERRTREIIENFDVRPPNPQLAAKALSGGNQQKLIIGREFELNPKLLLVSQPTRG
                     VDIGAIEFIHRKLIALRDAGSAVLLVSAELEEVTALADRLLVIYKGKFVGEVNPKETS
                     NEEIGLLMTGGR"
BASE COUNT          380 a          360 c          379 g          399 t
ORIGIN      
        1 gatgtacaga aaaccgcccg cgtgagcgcg ttcgtccgtg atgccgcgtc cggtcgtgcc
       61 gcttgagccg acaaaattga aaatcacgca gttttcgata tagacggctt tcgcgttcag
      121 aatacggatg ccgttaacgc cggttcccgc gccgttgatg gtcagattgc gcaggataac
      181 cgtgtcggtc gaaagcgcgt tgacgataac gccggttgtt ccggcggcga gaatcgaagc
      241 catacccgga ttgacgccgt cgattaccat acttttcgtg atgttaatcg ttccgaaacc
      301 gcccgggtcg agcgcgttga tttcaccgtt ggcggctgtt ttggaaatcg ctccggcgaa
      361 ggttttacaa ggcgctgtgc ggctgcacgg gttaacgtcg tcgccgacgc ccgaaaccca
      421 ggtgcgcgtc gcttgagcgt taattgctgt tgtgaaagaa atagcgaagg caaaaattgc
      481 caatactcgc aaagacagat ttaatttgtt tttcatcgaa cctccagatg ttttagaaaa
      541 tagtaattgt tgaaatcaac agagttgatt tttcggtaga acttttgtgc gtctagctga
      601 gagacgaacg cgattttatt ttttgctaaa ctttttgatt tttttatggt cagcgcctgt
      661 tgaaataatt aatcgtcgga tggacgttta aaatgcaatt tgaaacttac gttaatcaag
      721 tagtgttccg gaaatgccgg caggcttaaa attatgaaat ttcaagagtt agaaatctcg
      781 gcacgaccgc atccggcagg aaataatatc aattttgata ttcgagctat caatgttgat
      841 acagagtagt caattcaggc tattttttta gcacaatcaa aatacaccaa aacgtcgaaa
      901 actcaaaacg tcgaaagcac aaagttcaaa aagcaaagcg taaaatgaag ttgtttcttc
      961 cattttgcgc tttgcttttt aaattttgcg tttaatctta acgtccgccg gtcatcagca
     1021 aaccgatttc ttcgtttgaa gtctccttcg ggttgacctc gccgacaaat tttcctttat
     1081 aaatcacgag caggcggtca gccagcgccg tcacttcttc gagctccgcc gaaaccagca
     1141 aaacggcgct gcccgcatcg cgcagggcaa tcagcttgcg gtgaataaat tcgatggcgc
     1201 cgatatcgac gccgcgcgtc ggctgcgaaa ccagaagcag cttcggatta agctcgaatt
     1261 ccctgccgat gattagtttc tgctggttgc cgcccgaaag cgcttttgcc gccaactgcg
     1321 gattcggcgg gcggacgtcg aaattctcga taatttccct ggtgcgtctt tcgatggcgg
     1381 acgcgttcag aaaacctccg gctgtcagcg gcgggcgata atgaacgccg agaatagaat
     1441 tttcttccaa atcggaatcg agcagcaaac cgcggttgtg acggtcttcg ggaatatgcg
     1501 cgacgcccag ttccttgc
//
LOCUS       DASWNE010000824         5488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_559180, whole genome shotgun sequence.
ACCESSION   DASWNE010000824 DASWNE010000000
VERSION     DASWNE010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5488
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559180"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="VGB68_16125"
     CDS             <1..461
                     /locus_tag="VGB68_16125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050821.1"
                     /translation="GILSATTRNDLRQRIGADPKSRLTVNALEIGGEQVAEAVNHREV
                     SLTGLSERLKGVIAIAAEPVLVGGVGGRAAIMSALPESFTTTDEVHSFVASLLKHDQI
                     AFEQKRPKTRRTGVIGAASAPNAPPTTHIVRTRGGRKILERVRFVCRCCG"
     gene            complement(477..1382)
                     /locus_tag="VGB68_16130"
     CDS             complement(477..1382)
                     /locus_tag="VGB68_16130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050822.1"
                     /translation="MIRVLMLDLGDTLIDGNKHLFAHVPQALEILRGFETEAHEPLSL
                     CLVSDYYMPTADRTVEDIFQEYVSLLENLNLRQFFEPVERHVTLSTQAGVNKPKRRIF
                     ELAVERLGLDARLDECLFITENAGHTKSARRLGIKTLLFGETGAEAGFGDWSEAPLLI
                     AQMVNPESMSNLESALRLRLAVEHDLELIFVSEKSGDGIVRAQANKSHSLPVSGSESG
                     RNSIVTIPVNVEVEMNNRGRILAVDSEEPDKETLDEAAHYIKTLDDNKQISRKKGPLG
                     AGETHRETVNEKGEKRIKRERFTAF"
     gene            1675..2409
                     /gene="pgsA"
                     /locus_tag="VGB68_16135"
     CDS             1675..2409
                     /gene="pgsA"
                     /locus_tag="VGB68_16135"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEY0050823.1"
                     /translation="MNLPNYLTLARIVLVPLLVVILLTPFADEWFGISSYALAIAIFL
                     AASVTDILDGHLARRRNQVSNFGKLLDPIADKLLVSAALIVLVEKHLAPAWAVVVILG
                     REFIITGLRSVAASEGIVIAAQKIGKIKMWAQCVAIVALLVAGATGNPPVSNFGWSLP
                     TFRFWEVAEVQTAFAHLFSFSVNTDDWRVFGYLVGRGALWIAVITACWSMLDYFKYFF
                     VENKKRKTLAEAVFAKQETAEITSGK"
     gene            complement(2419..2697)
                     /locus_tag="VGB68_16140"
     CDS             complement(2419..2697)
                     /locus_tag="VGB68_16140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050824.1"
                     /translation="MKKPFWVKVGLFGVKSRGASIAWLYLLIALFILSPVIIFAVLTL
                     TKDTPLFPAIVRSLTGGALVFLTCVWSWLCIKWMDDNKGWAQIKSGSK"
     gene            complement(2777..3985)
                     /locus_tag="VGB68_16145"
     CDS             complement(2777..3985)
                     /locus_tag="VGB68_16145"
                     /inference="COORDINATES: protein motif:HMM:NF025198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HEY0050825.1"
                     /translation="MSTIRCTQCNLTNFATAVACKRCGYFFQPPAEGSPINPAAQSSF
                     EFGGQQQQAFNEFQTSVQPAGYTPPPQFPPPPRQNYYRQQQQYQSYQINKQKSGLAIA
                     SLVLGLIGCFVTSPIGLILGIVAMVKANRRPNEYGGKGLAIAGIVLNGLGLLFLPVIV
                     AIAVPNLMAARRAANEGSAIATIRTLSDAEQKHMASMFGKCGDIQTLIATKLFNDLSL
                     AKNEKNGYRFMVVNLPAGGCEIHATPLSTSHGTRSFFYSTEDNVIRAADKKGLAADKQ
                     DLPLGNSLREEREESLWGQPPSEYGALSTIRMLHSAQMTYAATVGNGSCGSLKDLANS
                     QLIKNDLADGEEGGYRFAVKKISQFGCELTATPVSGTAARSFYIGNEGVVRGKAKNGV
                     PADKNDPPIY"
     gene            complement(4099..5064)
                     /locus_tag="VGB68_16150"
     CDS             complement(4099..5064)
                     /locus_tag="VGB68_16150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050826.1"
                     /translation="MFLAVLASTVSASGQEPFRQQPRATPTPKLVINRIQRELPLAAR
                     NNLYCAGYIQNAPVNTDFEVVGASDEREQNIYAENDYVYISRGANRGVKVGDMFAVTR
                     PRGKFRTRFSRKGNLGIYVEEVGAVEVVNVKSEVSVARVMTSCDSFLLGDLLQPITNR
                     VSPVFEPRPMFDLFAEPTGKARGRIVLARDGREALSAEQVVYIDLGAEDNVRIGDYLT
                     IYRPLGTGGVLNSDQHISLSARDDGFQSERYRGGKFSNQAPRKSGSEAEGKIVSRRDA
                     KRRRPQDLRKIVGEMVILNVRERTATALITRNAQEIHTGDMVELQ"
     gene            5345..5420
                     /locus_tag="VGB68_16155"
     tRNA            5345..5420
                     /locus_tag="VGB68_16155"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5378..5380,aa:Thr,seq:tgt)
BASE COUNT         1238 a         1487 c         1396 g         1367 t
ORIGIN      
        1 gaggaattct gtcggcgacg acgcgcaacg acctccggca gcgaatcgga gccgacccca
       61 aatccagatt gaccgtcaac gctttggaaa tcggcggaga acaggtagcg gaagccgtca
      121 atcacaggga ggtttcgctg acgggactga gcgaaagatt aaaaggagta atagccattg
      181 cagccgagcc ggttctggtc ggcggagtcg gcggcagggc ggcgattatg agcgctcttc
      241 cagagtcttt cacgacgacc gacgaggtgc acagctttgt cgccagtctc ttaaagcacg
      301 accaaatcgc ttttgaacaa aaacgcccga agactcgccg gacgggcgtg attggcgcgg
      361 ctagcgctcc gaacgctccg ccgactactc atatagtgcg gacgcgcggc ggcaggaaaa
      421 tcctggagcg tgtgcggttt gtgtgccgct gctgcggata atggcgaaag ctttatttaa
      481 aaggctgtga agcgctcgcg cttgatgcgc ttctcgcctt tttcattaac cgtttcccgg
      541 tgcgtctcgc ccgcaccgag cggtcctttc ttacgcgata tctgcttgtt atcatccagc
      601 gttttaatgt aatgagccgc ctcgtccagc gtttccttat cgggttcttc gctgtcgacg
      661 gctaaaattc gtccccggtt attcatctct acctccacat ttacaggaat cgtcacgatg
      721 gagttccgcc cgctttcaga cccggaaacc ggcaaagaat gcgatttatt cgcctgagcg
      781 cggacgatgc cgtccccgga tttttcgctg acgaatatca gctccaaatc gtgttcgaca
      841 gccagccgca gcctgagagc gctttcgaga ttgctcatac tttcgggatt gaccatttga
      901 gcaatcagca gcggagcctc cgaccaatcg ccgaaaccgg cttccgcgcc ggtctcgcca
      961 aacagcagag tttttatccc caggcggcgt gcgcttttag tgtgtccggc gttttccgta
     1021 atgaataagc actcgtcgag cctcgcgtcc agaccgagcc gctcgacggc aagctcaaaa
     1081 atacggcgct tcggtttgtt tacgcccgcc tgcgtcgaaa gcgtgacgtg ccgctcgacc
     1141 ggctcaaaaa attgccgcag gtttaaattt tccaacaagc ttacgtattc ctgaaaaata
     1201 tcctcgaccg ttctgtccgc cgtcggcata taataatcgg aaaccagaca gagagataat
     1261 ggctcgtgcg cctcggtttc aaatcctcga agaatctcga gggcttgcgg aacgtgagca
     1321 aagagatgtt tatttccgtc gatgagcgta tcgcctaaat cgagcatcaa aacgcggatc
     1381 atagcaagcc tcctttacca gcccgccttt ctatcctgag agcgataccg caccgctctt
     1441 tgcaaagata gaatattgcg gcagactata ataattttat cacacttgtt aattaatcca
     1501 gccgctataa gcctgtcgga aaaatctcgg cgcatgtgtt ttataaattc gccgttatcg
     1561 accgaagccg cgccgtctcg cttaaaaatt aaatctttat gacatcgctc gtctttattc
     1621 ttgacacgtt ttttttgcac gcctaaactt tagcttgaga ggcttttaaa ccgagtgaac
     1681 ctgccgaatt atctaacttt agcgcgcatc gtactggttc cgctgctggt cgttatactg
     1741 ctcacgccgt ttgccgacga gtggttcggc atcagcagct acgcgctcgc catcgccatt
     1801 tttctcgccg cttccgtcac cgatattctc gacgggcatc tggcgcgacg ccgcaatcag
     1861 gtttccaatt tcggcaagct gctcgacccg attgccgata agctgctggt ttccgccgct
     1921 ctgattgtgc tggtcgaaaa acatctcgcg ccggcgtggg cggtcgtcgt gattttgggc
     1981 agagagttta ttattacggg tttgcgctcg gtcgcggcga gcgagggcat cgtcatcgcg
     2041 gcgcagaaaa tcggaaaaat aaaaatgtgg gcgcaatgcg tggcgattgt cgccctgctc
     2101 gtcgcgggcg cgaccggaaa tccgccggtc tcgaatttcg gctggagcct gccgacgttt
     2161 cgcttctggg aagtcgccga agtgcagacg gctttcgctc atcttttcag tttttccgtc
     2221 aataccgacg actggcgcgt tttcggttat ctggtcgggc gcggcgcgct ctggattgcc
     2281 gtcattacgg cttgctggtc gatgcttgat tactttaaat attttttcgt cgaaaataaa
     2341 aaacgaaaaa ctcttgccga agccgttttc gccaaacagg aaacggcgga aatcacttcc
     2401 ggcaaataaa gtttttcgtt atttagagcc tgattttatt tgcgcccagc ctttattatc
     2461 gtccatccat ttaatgcaca gccaggacca gacgcacgtc aggaaaacga gcgcgccgcc
     2521 cgtcaggctg cggacaatcg ccggaaacag cggcgtgtct ttggttaagg ttaaaacggc
     2581 gaatattatc accggagaca aaataaacaa cgctattaac aggtaaagcc aggcgatgga
     2641 agcgcctctg cttttgacgc cgaacagccc gactttcacc cagaacggtt ttttcataac
     2701 tcgtatcaaa atttatttcg gtcaagccgc gcttcgaaac aaaacggctg acgaactaac
     2761 gtttatgagc cggtttctaa taaatcggcg ggtcgttttt atcggcgggc acgccgtttt
     2821 tcgccttgcc gcgtacgacg ccttcattgc cgatgtaaaa agaacgcgcg gcggtgccgg
     2881 aaaccggcgt agcggtaagc tcgcaaccga attgagagat ttttttaacc gcaaaacgat
     2941 agccgccttc ctcgccgtcg gctaaatcat ttttaataag ctgcgagttt gccaaatcct
     3001 ttaaactgcc gcagctgccg tttccgaccg tagccgcgta ggtcatctgc gcgctgtgaa
     3061 gcatacggat cgtggaaagc gccccgtatt cgctcggcgg ctgtccccat aaagattctt
     3121 cccgctcttc gcgtaaactg ttgccgagcg gcaaatcctg tttatcggcg gctaagcctt
     3181 ttttgtcagc cgcgcgaatt acattgtctt cggtcgaata aaaaaatgag cgcgtgccgt
     3241 gcgaagtcga aagcggcgtg gcgtgaatct cgcagccgcc ggcgggaaga ttgaccacca
     3301 taaagcgata gccgttcttt tcgtttttcg ccaggctcag gtcgttaaat aatttcgtcg
     3361 caatcagcgt ttgaatatcg ccgcatttgc cgaacatcga agccatatgt ttttgctcgg
     3421 cgtcggaaag cgtgcgaatg gtcgcaatcg cgctgccttc gtttgccgcg cggcgcgccg
     3481 ccatcagatt cggaacggcg atggcgacga taaccggcaa aaacaaaagc cccaaaccgt
     3541 tcaggacgat gcccgcaatc gccagcccct ttccgccgta ttcattcgga cgtcgattgg
     3601 ctttcaccat cgcgacgatg cccagaatca atccaatcgg cgaagtaaca aaacagccga
     3661 taagaccgag caccagcgaa gctatcgcca aaccgctttt ctgtttgttt atttgataag
     3721 actggtattg ctgctgctgc cggtaataat tttgtcgcgg cggaggcgga aactgcggcg
     3781 gaggcgtata gccggcgggc tgaacagatg tctgaaattc attaaaagcc tgctgctgct
     3841 gcccgccgaa ttcgaaagaa gattgcgccg ccggattaat cggagagcct tctgccggag
     3901 gctggaaaaa ataaccgcag cgcttgcagg cgaccgccgt ggcaaagtta gtcagattgc
     3961 attgcgtaca tctgatagtg ctcatatcgt tttaaagatg tgtaaaaaaa gcaggcggaa
     4021 agcggaaagc gcaaggcaga caaatttctg gctgcttact gccttccgct ttccgctttc
     4081 cgccttcata tcaatttatt attgaagctc gaccatatcg ccggtgtgaa tttcctgcgc
     4141 gttgcgcgta atcaaggcgg ttgcggtgcg ttccctgaca tttaaaatca ccatctcgcc
     4201 gacgattttt ctcaaatcct gtgggcgacg gcgtttcgca tcgcggcgcg agacgatttt
     4261 tccttcggct tcgctgcccg atttgcgcgg cgcttgattc gagaattttc cgccgcgata
     4321 acgctcgctt tgaaatccgt cgtcgcgggc gctcagcgat atgtgctggt cggaatttaa
     4381 aacgccgccc gttccgagcg gacgataaat ggtcagataa tcgccgatcc gcacgttgtc
     4441 ttccgcgccc aaatcaatat aaacaacctg ctcggcggat aaagcttctc gcccgtcgcg
     4501 cgccagcacg attctgccgc gggcttttcc cgtcggctcg gcaaaaaggt caaacatcgg
     4561 gcgcggctca aacaccgggc tcacccgatt cgtaatcggc tgaagcaaat cgccgagtaa
     4621 aaagctgtcg cacgaagtca tcacgcgagc caccgaaact tccgatttga cgttgacgac
     4681 ttcgaccgct ccgacttctt cgacgtaaat tcccagattt ccctttctgg aaaaacgcgt
     4741 tctgaatttt ccgcgcggac gcgtcaccgc aaacatatcg ccgaccttaa cgccgcgatt
     4801 cgcgccgcgg ctgatataaa cgtaatcatt ttcggcgtaa atgttttgtt ccctttcgtc
     4861 gctcgcgccg acgacttcaa aatcggtatt gaccggcgcg ttctgtatat atccggcgca
     4921 atagagattg tttctcgccg ccaagggcag ctctctctgt attcgattga tgaccaactt
     4981 cggcgtcggc gtcgctcttg gctgctgccg aaacggctcc tgcccggagg cgctgaccgt
     5041 cgaggcaaga accgccaaaa acattatcgc aagacttttc gcactaatct gcaaagcttt
     5101 cactggaatt tctcctttta tttgtccgag attattattt gataacaagt aaagaggtct
     5161 tttggaaaag atgtctgaac accctttgcg ggcatattct atcgtaaccg ttttgcaaaa
     5221 caatcgtcaa caaatatttt aacggttaaa cgaatcggga aaattctttt tcctgcttgc
     5281 gaaaaatttc cggtttttgt agtattttcg tttgcggctg cgaaaggtcc cgcggcaaaa
     5341 acaagccggt gtagctcagt tggtagagca gttgatttgt aatcatcagg tcggcggttc
     5401 aagtccgttc accggctcca atgttttcaa tagtttaggg catctgaccg gatgcccttt
     5461 tcccttttgg tgtgtcaatg gtgggtca
//
LOCUS       DASWNE010000825         2964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_275685, whole genome shotgun sequence.
ACCESSION   DASWNE010000825 DASWNE010000000
VERSION     DASWNE010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275685"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..973)
                     /locus_tag="VGB68_16160"
     CDS             complement(77..973)
                     /locus_tag="VGB68_16160"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0050827.1"
                     /translation="MHIETLKVFCDLVDSQSFSLAAERNFITQSAVSQQIRSLEDRFK
                     RRLLERVRGRREVRLTPAGEVFYRESKNVLGGYDQLQESMRGLVGTISGTVKVATVYS
                     VGLHELPVKVRDFMTKFPSAKIDLEYSRTTRVVRDVLSGIVELGVVAFPEPRRGLTIT
                     QMQSDRLVLICPPDHEFAEREQVKSKELKGRDFVLFERDVPTRKATDKILKSYGVEVR
                     KAAEFDNIETIKRAVEVGFGLAIVPQPSVMDEEKNKQLAVIQLAEKEWVRPVGVIYRS
                     DRQLSIAAKKFVQLLENGSLKI"
     gene            complement(1016..1366)
                     /locus_tag="VGB68_16165"
     CDS             complement(1016..1366)
                     /locus_tag="VGB68_16165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEY0050828.1"
                     /translation="MRDSVLLEKSYNFALRMIKLFKFLAEEKREYVLSKQLLRSGTSI
                     GANIEEANQAESKVDFIHKLSIANKEAFETNYWLRLLRDSDILTKKQAESLIDDCEEL
                     QKLLTSSIKTSKNI"
     gene            1583..2314
                     /locus_tag="VGB68_16170"
     CDS             1583..2314
                     /locus_tag="VGB68_16170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050829.1"
                     /translation="MAIKISRTFILRKLHQLTGIVPLGAFFFVHLFTNSKAMSGEKVF
                     NDAVADIHHIPYLLFVEIGGIFLPLLFHSVYGIFISMEARANVGNYGYGRNWFYVFQR
                     ATGVFLFFFLLFHILNLRFGMIPGLESYGNPVAGNADKAFDIVAAEFKNIAILIFYIL
                     GVTATAWHLAYGFWLFALDWGILIGERAQKMALYASIGLAIFLSAVGINAAIAFVKPC
                     GLLPRALCEEAEKKGVVEPNAPRRF"
     gene            2398..>2964
                     /locus_tag="VGB68_16175"
     CDS             2398..>2964
                     /locus_tag="VGB68_16175"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0050830.1"
                     /translation="MASKGIRIAVIGGGLAGLAAAMKIAEAGHDVDLFSVVPVKRSHS
                     VCAQGGINGAVNTKGEGDSPWKHFDDTVYGGDFLANQPPVQKMCEMAPQIIYLFDRMG
                     VPFSRTREGLIDFRRFGGTLHHRTAFAGASTGQQLLYALDEQVRKWESAGKVTKYEGW
                     EMMSLVLDDHQVCRGLVAMNLQTLELKAF"
BASE COUNT          723 a          691 c          747 g          803 t
ORIGIN      
        1 gggagatttc gcccgctcaa gacgcaaaag gcgcgaaaga ataaatcttc aaatattttt
       61 taaaataacg gcgcgtctaa attttcagag agccgttttc cagaagctgg acgaattttt
      121 tcgccgcaat cgaaagctgc cggtcgctgc ggtaaatcac gccgacgggg cggacccatt
      181 ctttttcggc gagctggatg accgccaact gtttgttttt ttcctcgtcc atcaccgacg
      241 gctgcgggac gatcgccaag ccgaaaccga cttcgacggc gcgtttgatg gtttcgatgt
      301 tgtcgaactc ggcggctttg cgcacctcga cgccgtagga ttttaaaatt ttgtcggtcg
      361 ccttgcgcgt cggcacgtcg cgctcgaaaa gaacgaaatc gcgtcccttc agctctttcg
      421 atttaacctg ctcgcgctcg gcaaactcgt ggtcgggcgg gcaaatcaaa accaggcggt
      481 cgctctgcat ctgcgtaatc gtcaagccgc gccgcggttc gggaaaagcg acgacgccga
      541 gctcgacaat tccgctcagc acgtcgcgca ccacgcgcgt cgtccgcgaa tattccaaat
      601 caattttggc ggacggaaat ttcgtcataa aatcgcgcac tttgacgggc agttcgtgca
      661 gcccgacgct gtaaacggtg gcgactttga cggttccgct aatcgtgccg accagcccgc
      721 gcatactttc ctgaagctgg tcgtagccgc cgagaacgtt tttcgattcg cgataaaata
      781 cttcgcccgc cggcgtcagg cgaacttcgc gccgaccgcg cacgcgctcc agaaggcggc
      841 gcttaaagcg gtcttcgagc gagcgaatct gctggctgac cgccgattga gtaataaaat
      901 tacgctccgc cgccagcgaa aaactctgcg aatcgaccag gtcacaaaat acttttaagg
      961 tttcgatatg cataaattta actgataact gataactgat aactgataat gtatttcata
     1021 tattttttga ggttttgatt gaagaagtta gaagtttttg tagttcttca caatcgtcta
     1081 ttaaagattc tgcttgcttt ttagttaaaa tatcgctgtc tcgtaaaagt ctgagccagt
     1141 aattagtttc aaatgcctct ttgtttgcaa tcgaaagctt atgtataaaa tcaactttcg
     1201 actcagcctg atttgcttcc tcgatattag ctccaatgga tgttcccgaa cgcagcagtt
     1261 gttttgacaa gacatattcc cgcttctctt ctgccagaaa cttaaacaac ttaatcattc
     1321 ttaaggcaaa gttataagat ttttccaata atacgctgtc tctcaaaata aacctcgaaa
     1381 ctatcagtta tcagttatca gttatcaact gaaataataa gtttttctac acaatttctg
     1441 caagaaaatt cattttatct tataaatcaa aaaacgtatg atgataaata tgtgattgcg
     1501 gattgccgat ttacgtttca gaatctaaaa tttagagaca cgcagcaata ttcgcgattc
     1561 aaatttaatc gaggcttggg aaatggctat caaaatcagc aggactttca ttttaagaaa
     1621 gctgcaccag ttgaccggca ttgtgccttt gggagcgttt tttttcgtcc atcttttcac
     1681 gaattcaaaa gcgatgagcg gcgaaaaggt ttttaacgat gcggtcgccg atattcatca
     1741 tattccgtat ttattgtttg tcgagattgg cggaattttc ctgccgcttt tgtttcattc
     1801 ggtttacggc atttttatct cgatggaagc gcgggcgaac gtcggaaact acggttacgg
     1861 gcgcaactgg ttttacgttt ttcagcgcgc gaccggcgtt tttcttttct tctttctgct
     1921 gtttcatatt ttaaatttgc gtttcggaat gattccgggc ttggaatcat acggcaatcc
     1981 ggtggcggga aatgccgaca aagcgtttga tattgtcgcg gcggaattta aaaacatcgc
     2041 tatcttgatt ttttatattc tcggcgtgac ggcgacggct tggcatctgg cttacggctt
     2101 ctggctgttt gccctcgact ggggaatttt gattggcgag cgggcgcaga aaatggcgct
     2161 ttacgcgtca atcggattgg cgatttttct gtcggcagtc ggaattaacg cggcgatcgc
     2221 gtttgtcaaa ccgtgcggct tattgcccag agcattgtgc gaagaggcgg aaaagaaagg
     2281 cgttgtcgaa ccgaacgcgc cgagaagatt ttaattaact gaaagtggat aactgataac
     2341 cgatagtgaa aaactaagaa ctatcagtta tcagttatca gttatcaact aaattttatg
     2401 gcttcaaaag gtatcaggat agcggtcatc ggcggcggtc tggcggggct ggcggcggcg
     2461 atgaaaatcg ccgaagcggg acacgatgtc gatttgtttt cggtcgttcc ggtcaaacgc
     2521 tcgcactcgg tttgcgcgca gggcggcatc aatggcgcgg tcaatacaaa aggcgaaggc
     2581 gattcgccgt ggaaacattt cgacgacacg gtttacggcg gcgattttct ggcgaaccag
     2641 ccgcccgtgc aaaaaatgtg cgagatggcg ccgcaaatca tctatctgtt tgaccggatg
     2701 ggcgttccgt tttcgcggac gcgcgaaggc ttgattgatt tccggcgctt cggcggaacg
     2761 cttcatcacc gcacggcgtt tgccggcgct tcgaccggac agcagctgct ctacgcgctg
     2821 gacgaacagg tgcgcaaatg ggaatcagcc ggaaaggtga cgaaatacga aggctgggaa
     2881 atgatgtcgc tcgttttgga cgaccatcag gtctgccgcg gactggtggc gatgaatctg
     2941 caaacgctgg aactgaaagc tttt
//
LOCUS       DASWNE010000826         1636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_167083, whole genome shotgun sequence.
ACCESSION   DASWNE010000826 DASWNE010000000
VERSION     DASWNE010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167083"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..1326
                     /locus_tag="VGB68_16180"
     CDS             202..1326
                     /locus_tag="VGB68_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050831.1"
                     /translation="MGEHSPLQWLEQALGALLLLLILTDIFLIVLYARIGSGIISNRL
                     AHLVWRLFRLVSKPFGSYRGIILSFCGPIILIFLVIFWALTLTCAAAMLIHPNLGSSV
                     KSGSGETPTDFITALEAGGGSLAIVGASDFSPQSGGFRLLYLFNSLIGMSVVSLTLTY
                     LMQVYNALQRRNALGLRAYLLTAETGDAAELVAGLGTDGQFSSGYSDLANWAGEMSRV
                     KESHHFYPVLFYFRFKESFYSTSQMTLIAFDTVSIIKSALDDEEYSWLKNATAVTQIR
                     RAAMMLVTTLSDTFLPSGTPDDQNEPDEETLERWRKRYSAALQKLREANIKTIDDEES
                     GAETYIALRREWEHYITALAPTMAYRMDEIDPAGNHPREK"
BASE COUNT          397 a          412 c          441 g          386 t
ORIGIN      
        1 aaacaggact cttgtattaa agtcgagctc aagccgggaa taaacggcaa cgatggagcg
       61 cgctggaaaa aacggataga gtttaccgat aatcgttcag acgccggcgg gaacggctaa
      121 atcgaagccg ttttcgggct cgcttccggt tttggtcaca ctaacttcac gaataatgtc
      181 agcgaggttt tgaaaaacgt catgggcgaa cacagtccgc ttcaatggtt agagcaggcg
      241 ctcggcgcgc tgctgctgct gcttattctg acagatatat ttctgattgt gctttacgcg
      301 cgtatcggca gcggcatcat cagcaaccgg ctggcgcacc tggtgtggcg tcttttccgt
      361 ctggtatcaa aacctttcgg cagctatcgc ggcatcatct tgtctttctg cggtccgatt
      421 attctcatct ttctggtcat cttctgggcg ttgacgctga cgtgcgccgc ggcgatgctt
      481 attcatccga atctgggttc gtccgttaaa tccggcagcg gcgagacgcc gacggatttt
      541 atcacggcgc tggaagcggg cggcggcagt ctggcgatag tcggggcgag cgatttttcg
      601 ccgcagtcag gcggattccg tctgctttac ctgtttaact cgcttatcgg aatgtcggtc
      661 gtctcgctga ctttgacgta cctgatgcag gtttacaacg cgctgcagcg gcgaaacgcg
      721 ctcggattgc gggcttacct gctgactgcc gaaacgggcg atgccgccga actggtcgcc
      781 ggactgggaa ccgacggaca attcagcagc ggttattcgg atttggcaaa ctgggctggc
      841 gaaatgtcga gagtcaaaga atcgcatcat ttctatccgg ttttgtttta tttccggttc
      901 aaagaatcct tctattcgac gtcgcagatg acgctcatcg cctttgatac ggtgtcgatt
      961 atcaaaagcg ctctggacga cgaggaatat tcgtggctga agaatgcgac ggctgtgacg
     1021 cagattcggc gcgccgcgat gatgctcgtc acgacactgt ccgacacttt tcttccgagc
     1081 ggaacgcccg acgaccagaa cgagcccgac gaagaaacgc tcgaacgctg gcgcaaacgc
     1141 tactccgccg ctttgcagaa actccgggaa gcaaacataa aaacaatcga cgacgaggaa
     1201 tccggcgcgg aaacctacat cgcgctgcgc cgcgaatggg agcattacat cacggcgctc
     1261 gcgccgacga tggcttaccg gatggatgaa atcgacccgg cgggaaatca tcccagggaa
     1321 aaataaaatc ggaaattggc gggcaggcat ataaaagcgc ctttttcata aacgtccatt
     1381 cgtctgataa atcagacggc gaatccatcg aaatacctgc tccggcgaga atggcgattt
     1441 ttctatccgg tgtaaataaa ttatccgaac gcatcgcagc ggtcataatt tatccaatta
     1501 atgcgcgggt atttaatttg ggtctatggt agcaaacaag caatgataaa cttgtaagct
     1561 actgtaatga aacaactaaa ccgatattat cggtgcgcta aaatttctga gcggaaaatt
     1621 cgtcaaatca tcaagt
//
LOCUS       DASWNE010000827         4166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_449674, whole genome shotgun sequence.
ACCESSION   DASWNE010000827 DASWNE010000000
VERSION     DASWNE010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449674"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VGB68_16185"
     CDS             <1..260
                     /locus_tag="VGB68_16185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050832.1"
                     /translation="AGLLQKFVTDSDPFTLSEDFPKAFRVGGCKTLAPGKTESQVLLF
                     WKDDVRSEQQELKIEAVKQNDKWLISNISNNTSNLQNILNH"
     gene            268..1668
                     /gene="gatA"
                     /locus_tag="VGB68_16190"
     CDS             268..1668
                     /gene="gatA"
                     /locus_tag="VGB68_16190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEY0050833.1"
                     /translation="MSEIKNSVEKTLDNAEKLNETLNSFLTVEREYAIKRAAEVENNP
                     ENVKLKGFAVAVKDNICTDWTQSSCGSHILGNYRPQYNATVVEKLNAAGAIIVGKTNM
                     DEFAMGSSNENSAFGVVRNPWDTERVPGGSSGGSAVAVASGVVRASLGSDTGGSIRQP
                     ASFCGVFGIKPTYGRVSRYGLVAFGSSLDQIGVFGQTAKDVAEVMKIIAGRDAHDATT
                     ADVPVPNYSTELEKDIKGLKIGVPRALFGEGLDDEVRNAVEQAIENYRALGAEIVDVE
                     LPLVKYGIAVYYIIATAEASSNLARFDGVRYGFRAEESGGLRQMYFKTREEGFGAEVK
                     RRIMLGTYVLSSGYYDAYYAKAQKVRTLLKNDFQTAFQKSDAIITPTSPTVAFKIGEK
                     SDDPLAMYLNDVYTVSANLAGIPGISVPCGLSSDGLPIGLQLLGNNWSESLLLNLSNV
                     YEKSFPLKAKPKIFAE"
     gene            complement(1844..4069)
                     /locus_tag="VGB68_16195"
     CDS             complement(1844..4069)
                     /locus_tag="VGB68_16195"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0050834.1"
                     /translation="MENKERNNNAYASILLPIGFAALAWSIYAFPVEKISAGLIALAV
                     VTVFFSSYLGIQLPRTKIHLTISDALILLSLLIYDGEVAVLLAGLESCYTSLKFRSKG
                     INIKTKTVFINIAIASVSTFLTAQVVHWFFGSTQEIISSGSATVFIGLLTVMALTQFI
                     VNSICVAGFAALKSGKSVWQVWNEYCLNALVMFLTGAVLAGVGAKAIQQINMFLFAVI
                     IGFFAVAYMTYRRYVDDVKETAAKAEQAERERAEQAERHIVELQHHIAEQDRISQALR
                     ESKERFRHAAFHDALTDLPNRNQFIETLKFQLEKSRQTPNFNFAVLFLDLNRFKTINE
                     SLGHSTGDNLLKKVGERLKKLIGEDDMLARFGGDEFGIVLSQVKTTEEAVSFAQLINY
                     KLSSPFAIGGRQVFITTSIGIALGNPTYHAAEEVLRDADIAMYYAKDHEKNYEIFDQS
                     MHTRAVTLLQIETDLRYAVERNELLAYYQPIIDLETMQLGGFETLIRWNHPQRGLVSP
                     GDFIPVSEETGLIVPITLWILRESCRQMVAWQNLDAANKPLILSVNLSGKHFAQGNLV
                     EEIQKIIVETGISPSCLKLEITESAVMENAELVIAMLKQLKELGLQLSIDDFGTGYSS
                     LSYLHRFPIDTLKVDRSFVGTMEDGTENGEIVRTIIALAKALGMDVVAEGIETIHQLH
                     QLRILGCEYGQGYLFSRPVPHDEAEKIVLDKTRWQSILPTPKLVIPPHSKEIPHLRLA
                     K"
BASE COUNT         1019 a         1065 c         1109 g          973 t
ORIGIN      
        1 tggcggggct tttgcaaaaa ttcgtcaccg attccgaccc gtttacgctt tccgaagatt
       61 ttccgaaagc gttccgcgtc ggcggctgca aaacgctcgc gcccggcaaa accgaatcgc
      121 aggttttgct tttctggaaa gacgacgtgc gcagcgagca gcaagagctg aaaatcgaag
      181 ccgtcaaaca aaacgacaag tggctgataa gcaatatttc caataatacg agcaatttac
      241 aaaatattct gaatcattaa tttttcaatg agcgaaataa aaaattccgt cgaaaaaact
      301 ttagacaacg ccgaaaaact gaatgaaacg ctcaactcgt ttctgaccgt cgagcgtgaa
      361 tacgcgataa aacgcgccgc agaagtcgaa aacaatccgg aaaacgtcaa attaaaaggc
      421 tttgccgtcg ccgtcaaaga caacatctgc accgattgga cgcaatcctc gtgcggctcg
      481 catattctgg gaaattatcg cccgcagtat aacgcgacgg tcgtcgaaaa actgaacgcg
      541 gcgggcgcaa tcatcgtcgg caaaacgaat atggacgaat ttgcgatggg ttcgtcaaac
      601 gaaaattccg cgttcggcgt cgtccgcaac ccgtgggaca cggagagggt tccgggcgga
      661 agctcgggcg gttcggcggt ggcggtcgcc tccggcgtcg ttcgcgcttc tttaggctcg
      721 gacacgggcg gctcgattcg ccagccggct tctttctgcg gcgttttcgg catcaagccg
      781 acttacggca gagtttcgcg atacgggctg gtcgcgttcg gttcttcgct cgaccaaatc
      841 ggcgttttcg gacagacggc gaaagacgtg gcggaagtta tgaaaatcat cgccggacgc
      901 gacgcgcacg acgcgacgac ggcggacgtt cccgtgccga attattcaac cgagcttgaa
      961 aaagatataa aaggcttgaa aatcggcgtt ccgcgcgcgt tgttcggcga aggcttggac
     1021 gacgaagtca gaaacgcggt cgagcaggca atcgaaaact atcgcgctct gggcgcggaa
     1081 atcgtcgacg tcgagctgcc gctggtcaaa tacggaattg ccgtttatta cattatcgcc
     1141 acggctgaag cgtcgtcgaa cctggcgcgt ttcgacggcg tgcgttacgg tttccgggcg
     1201 gaagaatcgg gcggcttgcg ccagatgtat ttcaaaacgc gcgaggaagg tttcggcgcg
     1261 gaagtcaaac gccggattat gctcggaact tacgttttgt cgagcggtta ttacgacgct
     1321 tattacgcga aagcgcaaaa ggtgcggacg cttttaaaaa acgattttca aaccgctttt
     1381 caaaaatccg acgccattat cacgccgact tcgccgaccg tcgcctttaa aatcggcgaa
     1441 aaatcggacg acccgctggc gatgtatctg aacgacgttt acacggtttc ggcaaatctg
     1501 gcgggcattc ccggcatttc cgtgccgtgc ggtctgtcgt cggacggctt gccgatcggt
     1561 cttcagcttt tggggaacaa ctggtcggaa agcctcttgc tgaatctttc aaacgtttac
     1621 gaaaaatctt ttccgctcaa ggcgaaaccg aaaattttcg ccgagtagaa tgaacccgcc
     1681 gcaaaaatca cgaaagaagg aatcgacagt gataattaaa gttaaataaa tctttattat
     1741 caccgtcgat tccttctctt tcgtgttttt ctctatgctc tgcgccggct aaaattcttt
     1801 gttcttcgtt cgtgcttttt cgcgtatggt cgcggctgaa tctctatttc gccaatctga
     1861 gatgcggaat ttctttcgaa tgcggcggga tgacgagctt cggcgtcggc agaatcgact
     1921 gccagcgcgt tttgtccaga acgatttttt cggcttcatc gtgcggcacg gggcgcgaaa
     1981 acaaatagcc ctgaccgtat tcgcaaccca ggattcgaag ctggtgaagc tggtgaatcg
     2041 tttcgattcc ttcggcgacc acgtccattc cgagcgcctt ggcgagcgcg ataatcgtgc
     2101 ggacaatctc gccgttttcc gttccgtctt ccatagtgcc gacaaaagag cggtcgactt
     2161 tcagcgtatc gatcgggaaa cgatgcaggt agcttaaaga agaataaccc gtgccgaaat
     2221 cgtcaatcga aagctgcaag ccgagttctt tcaattgttt cagcatcgca atcaccagct
     2281 cggcgttttc catcacggcg ctttccgtga tttccagctt cagacaggac ggcgaaattc
     2341 cggtttcgac aataattttt tgaatttctt cgaccagatt gccctgcgca aagtgtttgc
     2401 ccgaaagatt gacgcttaaa atcagaggtt tattcgccgc gtcgaggttt tgccacgcaa
     2461 ccatctggcg gcaggattcg cgcagaatcc acagcgtaat cggcacgata agaccggttt
     2521 cctcgctgac gggaataaaa tcgcccggcg agacgagccc gcgctgcggg tgattccagc
     2581 gaatcagcgt ttcgaagccg ccgagctgca tcgtttccaa atcgattatc ggctggtaat
     2641 aggcgagaag ctcgttgcgc tcgacggcgt agcgcaaatc ggtttcgatc tgcaaaagcg
     2701 tgacggcgcg cgtgtgcatc gactggtcga aaatctcgta atttttttcg tggtctttgg
     2761 cgtaatacat cgcgatgtcg gcgtcgcgca aaacttcttc ggcggcgtga taagtcggat
     2821 tgccgagcgc gattccgatg ctcgtggtga tgaaaacctg acgaccgccg atggcgaacg
     2881 gcgacgacaa tttgtaattg ataagctgtg caaagctgac ggcttcttcg gtcgttttga
     2941 cctggctcaa gacaatgccg aattcgtcgc cgccgaaacg cgccagcata tcgtcttcgc
     3001 caatcagctt tttcaatctt tcgccgactt tctttagcag attgtcgccc gtcgaatgcc
     3061 cgagactttc gttgatggtt ttaaaacggt taagatcgag aaacaagacg gcaaaattaa
     3121 aattcggcgt ctggcggctt ttttccagct ggaattttaa ggtttcgata aactggttgc
     3181 gattcggcaa atccgtcaaa gcgtcgtgaa aagcggcgtg gcgaaagcgc tccttgcttt
     3241 cgcgcagagc ctggctgatc ctgtcctgct cggcgatgtg atgctgaagt tcgacgatgt
     3301 ggcgctcggc ttgctcggcg cgctcgcgct cggcttgctc ggctttggcg gcagtttcct
     3361 tgacgtcgtc cacatagcgg cgataagtca tataagcgac ggcgaaaaat ccgataatta
     3421 ccgcaaacaa aaacatattg atttgctgga tggcttttgc gccgacgcca gccagaaccg
     3481 cgcccgtcag aaacatcacc agcgcgttta gacaatattc gttccaaacc tgccagacgc
     3541 tctttccgct tttcagcgcg gcaaaaccgg cgacgcaaat cgaattgacg ataaactgcg
     3601 tcagcgccat caccgtcagc aacccgataa aaacggtcgc gctgccgctt gagataatct
     3661 cctgggtcga gccgaaaaac cagtggacga cctgcgccgt cagaaaagtc gagaccgaag
     3721 cgatggcgat gttgataaag acggtcttgg ttttaatgtt gatgcctttg ctgcgaaact
     3781 tcagcgacgt gtagcacgat tccaaaccag ccagcaggac ggcaacctcg ccgtcgtata
     3841 tcaaaagcga aagcagaatc agagcgtcgg aaatcgtgag atgaattttc gtccgcggca
     3901 gctgaatgcc gagatacgag ctgaaaaaga ccgtcacgac ggctagggca atcagcccgg
     3961 cgctgatttt ctcgaccgga aaagcgtaga ttgaccacgc gagagcggca aagcctatcg
     4021 gcagtaaaat cgaagcataa gcgttattat ttctttcttt gttttccact tgttcggata
     4081 aatcgggaat ctgggaataa tttagaaggt atgtacaaga acctgaattt cggaaggcgc
     4141 ggctcggaaa agcaacttaa gccttt
//
LOCUS       DASWNE010000828         9681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_410121, whole genome shotgun sequence.
ACCESSION   DASWNE010000828 DASWNE010000000
VERSION     DASWNE010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9681
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410121"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1279
                     /locus_tag="VGB68_16200"
     CDS             <1..1279
                     /locus_tag="VGB68_16200"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0050835.1"
                     /translation="TEDNFGRIYVGTARGINRVEPETGRIKLYTQADGLPGSAVSRCA
                     RDEAGNLWFSQKFNLARLEVHADENFAPPIFIGDLRVNGETVKKLSELGETTVENLDF
                     NSEQRQIQIGFFALGFGTGETLRYQYKLAAADWSEPSAQRTVNLNLAPGSYNFLVRAV
                     NAEGTPSENPARVAFTIARPVWQRWWFLITAALVISFLIYAVYRYRVAQIIRLERVRT
                     RIATDLHDDIGSSLSKIAILSEVVRQKSGSTDANEPLAIIADTSREMVDSMSDIVWAI
                     NPERDKLSDLIQRMRRFAEELLDAQDIGYQFFVPENLKDVALGADVRREIYLIFKECV
                     NNLAKHSGASEAYMKVDLENESLVVEVRDDGRGFDQSAKSNGNSHGFGGNGLINMRRR
                     AESLGGALAIDSEPGKGAKIVLRVPVGKKLFAV"
     gene            1367..2059
                     /locus_tag="VGB68_16205"
     CDS             1367..2059
                     /locus_tag="VGB68_16205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0050836.1"
                     /translation="MLQQAIMQTIMTENNEAAAIIKVAIIEDQRDIREGLGTLISYTD
                     GFQCVGKYGSMEEALPGIRHRQPDVVLSDIGLPGMDGIEGIKRIKEQYPQMTILMLSV
                     YQDDDRIFHALCAGAVGYLLKKTPPTKILDSLREAVAGGAPMSPEVARKVITIFKDFR
                     PPEQVDYDLTPHEVRILKLLVEGHSYKTAAKQLNVTTSTVSFHLKNIYEKLQVHSKSE
                     AVAKALQHRLIK"
     gene            complement(2922..3965)
                     /locus_tag="VGB68_16210"
     CDS             complement(2922..3965)
                     /locus_tag="VGB68_16210"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0050837.1"
                     /translation="MKMQNLLAARRRVFLLISLAVLIPVFSANTYAQFGNTISGHIFG
                     AQREPLGDINVELLDSYSRFIGRARTNGSGRYIFTGLGSGRFVIRVLSSGTDYEEQEQ
                     EVEIVNFAVRSSNGDSRPSGFSNEQKDFYLRPRRRGELGVTGTIFAQEVPENAKKLYE
                     KGVAELGDKKEKEGLGNLKSAIEAFPKYYAALERLGTEYVRLGHFEAAEILLTLAVDV
                     NPRGYKSWYGLAYSLYSLKKNTEAGAAIQKAIEMNQYSAEAFLLSGVLKRQNKQFDES
                     EKQLVKAKELSKGTMPLVNWHLALLYGYDLKRYRDAARELKLFLKAQPDAKDGEKIRA
                     LIKEFEDKAQTAG"
     gene            complement(4030..4620)
                     /gene="ribA"
                     /locus_tag="VGB68_16215"
     CDS             complement(4030..4620)
                     /gene="ribA"
                     /locus_tag="VGB68_16215"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEY0050838.1"
                     /translation="MERVAAADLPTQTGKFKIVGYRSLNSSEEFVVLVKGEMKADVPT
                     LVRIHSQCLTGDVFGSIKCDCGPQLQRAMEMIEAEGRGAIVYQQQEGRGIGIINKIRA
                     YALQDEGADTVEANEKLGFAVDAREYQQCAEILFDLGLCKVRVMSNNPDKIRALEEAG
                     LQVLERVPIEVESEEPAAHYLKVKREKLGHLLNVDE"
     gene            complement(4860..5276)
                     /locus_tag="VGB68_16220"
     CDS             complement(4860..5276)
                     /locus_tag="VGB68_16220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050839.1"
                     /translation="MINKGKTTTTTTTEDLMIQGLLGNYLRVRSADAIISNDKHLDDD
                     SLTAFVEGNLSERESTPILGHLVDCSFCRQVTAELVRLDFAFAGEEAPVPVAQNAPTS
                     VSDVLNNLLSRIFGTSDSAVLAHQQPEEEDEDEKKS"
     gene            complement(5358..6386)
                     /locus_tag="VGB68_16225"
     CDS             complement(5358..6386)
                     /locus_tag="VGB68_16225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050840.1"
                     /translation="MSPETNQTDELLNQCEPVIEEVFARCDENAPNFGVRLECFKESL
                     KKTARKYLFADESNTPTIEEVCAFFREIQAGDLFMAIACANGNERAWWEFDHQHRAYL
                     ERVARHLASSEMNAQEVIDTVYVELYGTRVVDGERVSKFATYSGRGSLRGWLRTVVWH
                     SLVDLHRTSHDEVSLDEMTEKVGEGYTHSTFAEPVLGGETEMLDHLTRERYRKATVSA
                     IENAFSTLDNHEKLLLLYYHVENLKLREIARLVEVPTSPLRFWFQRKSDKRDQNPATR
                     IHESTIMRWLEKTYAKILKLFRDDLSSGLSLKNEEIELCMKIATQDLASPNLYRNLMT
                     TETPNMSV"
     gene            complement(6441..8285)
                     /locus_tag="VGB68_16230"
     CDS             complement(6441..8285)
                     /locus_tag="VGB68_16230"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0050841.1"
                     /translation="MSEEAKKFHEEEEIGKTYDLRVIRRLLRYLKPYWHIAAVALVLT
                     LLSNILLSLQPRFTQSAVDDFIVPKQADGIWLFAFAFFGLFLFRFIFSYFQEILVNNV
                     GQRVMFDLRTEIFTKLQHQEISYYDKHPVGRIITRITSDVDALNELFTSGVIDVLGDL
                     VIIFAIVGVMFWMDWRLALVTLVTVPLLFTATNWFRKHARRGFDRVRTRTAKLNAFLQ
                     EHISGAQSVQLFNAESKAQKRFHDINDDYRNANIETIYYYSIFYPLVDFISAVGIAAV
                     IFAFGAEYLSVVAAGGEPLTIGVLAAFIQYSGMLFQPIRDLSDKFNIFQAAIVASHRI
                     FILFDLPVQVETPQNPKKSGKAVGDIEFQNVWFAYKKEDWVLKDISFTFKAGESIALV
                     GATGSGKTTVTNLLMRFYDVQKGRILLDGVDLRDWDLQSLRENFAVVLQDVFLFSGTI
                     EDNIRLGKKEIADERTVWAAREVHAAEFVERLENGYKTELKERGAGLSVGQKQLISFA
                     RALAFDPKILVLDEATSSIDTETEQLIQAAVERVMRERTSFVVAHRLSTIQNCNRILV
                     FHHGELRESGTHHELLAVRGLYWRLYQLQYADRTAETPGLANPALSQT"
     gene            8375..9349
                     /locus_tag="VGB68_16235"
     CDS             8375..9349
                     /locus_tag="VGB68_16235"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEY0050842.1"
                     /translation="MSRFIDERKNNWQKLEGLLEILETSPLRGLPRAQVRELGELYRR
                     AASDLAIARAESRDPKLVNYLNSLVIRAHGRIYRADTKGANLVWQFFVRDFPQAFRRS
                     WRYTALAFATFTIFGIASFLLCYYDLNFADDLGLSSLRYSVQSDHKWWESLNDANQIG
                     SSEILTNNIMVAIRAFAFGAFFGIGTLFVLIFNGVYIGGVLGVCYAINPAFGNALVTF
                     MVGHGVIELSCIFIAGGAGMLIGYSMINPGDLSRTQALKKNGMEAARLAIGCACLLVV
                     AGIIEGFLSPSLLPAWIKYGTGILTGIAMYSYLFLVGREKEEKREEKI"
BASE COUNT         2386 a         2448 c         2404 g         2443 t
ORIGIN      
        1 cacggaagat aatttcgggc gcatctatgt cggcacggcg cgcggcatca atcgcgtcga
       61 gccggagacg ggacgcatca agctctacac gcaagccgac ggtttgcccg gcagcgccgt
      121 cagccgctgc gcgcgcgacg aagcgggcaa tttatggttt tcgcaaaaat tcaatctggc
      181 tcgcctcgag gttcacgcgg acgaaaattt tgcgccgccg atttttatag gcgatttgcg
      241 cgtcaacggc gagacggtga aaaaactatc ggagctcggc gaaacgacgg tcgaaaacct
      301 ggattttaat tccgagcagc ggcagattca aatcggattt ttcgcgctcg gtttcgggac
      361 gggcgagact ttgcgctatc aatacaagct cgccgccgcc gattggagcg agccttcggc
      421 gcaacgcacc gttaatttaa atctcgcgcc gggcagctat aattttctgg tgcgcgccgt
      481 caatgccgaa ggcacgccga gcgaaaatcc ggcgcgcgtc gcctttacga ttgcgcgtcc
      541 cgtctggcag cgctggtggt ttttgattac ggcggcgctc gtaatttctt ttttgattta
      601 cgcggtttac cgttaccgcg tcgcgcagat tatccggctc gaaagagtca gaaccagaat
      661 cgccaccgat ttgcacgacg acatcggctc gtcgctttca aaaatcgcga ttttgtccga
      721 agtcgtgcgg caaaaatccg gcagcaccga cgccaacgag ccgctggcga taatcgccga
      781 cacctcgcgc gagatggtcg attcgatgtc ggacattgtc tgggcgatta accccgaacg
      841 cgataaatta agcgatttga tacagcgaat gcgccgtttc gccgaagaat tgctcgacgc
      901 gcaggacatc ggctatcaat ttttcgtgcc ggaaaattta aaggatgtcg cgctcggcgc
      961 ggatgtgcgg cgcgagattt acctgatttt caaggaatgc gtcaacaatc tggcgaagca
     1021 ttccggcgcg agcgaagcct atatgaaagt cgatttggaa aatgaaagtc tggtggtcga
     1081 agtccgcgac gacgggcgcg ggttcgacca gtcggcgaaa tcgaacggga attcgcacgg
     1141 tttcggcggc aacggtttaa ttaatatgcg gcggcgcgcc gaaagcctcg gcggcgcgct
     1201 cgcgattgac tccgaaccgg gaaaaggcgc gaaaatcgtt ctgcgcgtgc cggtcggcaa
     1261 aaaattattt gccgtttaat aaaattcgct acgcgaatac gtggtgcaaa aacggcttaa
     1321 aaaaattatt ctgcttgcgt cagacatttt aaggaaaatt tttattatgt tacagcaggc
     1381 aattatgcaa acgataatga cggaaaacaa cgaagcggcg gcgattatta aagtggcgat
     1441 tatcgaagac cagcgcgaca ttcgcgaagg tttgggaacg ctcatcagct acacggacgg
     1501 attccagtgc gtcggcaaat acggctcgat ggaagaagct ctgcccggca ttcgccaccg
     1561 gcagccggac gtggttttgt ccgacatcgg cttgcccgga atggacggca tcgaaggcat
     1621 caaacgcatc aaagagcaat atccgcagat gacgattctg atgctctcgg tttatcagga
     1681 cgacgaccgc atttttcacg ctctgtgcgc gggcgcggtc ggttatcttt taaagaaaac
     1741 gccgccgacg aaaattctcg acagtctgcg cgaagcggtc gccggcggcg cgccgatgtc
     1801 gccggaagtg gcgcgcaaag tcatcacgat ttttaaggat tttcgaccgc ccgaacaggt
     1861 cgattacgat ttaacgccgc acgaagtccg tattttgaaa ttgctcgtcg aaggtcacag
     1921 ctacaaaacg gcggcgaaac agctcaacgt cacgaccagc acggtttcgt ttcacctgaa
     1981 aaatatttac gaaaaattgc aggtgcactc aaaaagcgaa gccgtcgcca aagccttgca
     2041 gcaccggctt ataaaataag gcgcgcggcg cgcggcggaa aatctgcgcg ccgggcgcga
     2101 ggcgttgaaa gtaaattttt ccgggcaatc cggattataa aaaatcaatt aatcgaataa
     2161 aaaagcgaat gaattataag atttcattcg cttttttatt tctccggaaa acctcaccgc
     2221 ccgcaaacct actgtgtaaa tacgcggcga gcgggcgatt tgaatttttt acaccggctc
     2281 gcgtcgcgtt tgaagaattt tattcggcga ggaaaaaact cgaattcaaa tgacattacg
     2341 ggtctggtgg gctttgcaga aaaataaaga aagggaaaat tgtaaattct aaattgtaaa
     2401 ttctaaattg atgttcggta tcttttgtga agcaattgag aattcataat tgagaattta
     2461 caattagcca tcggaatttt tctcgatttt ctttattttt gtgcaaagca tacgttttac
     2521 attttgcatc gttcattttt tgattataga tttcgtctga ttaatcgaga atgtaaaatt
     2581 gattaatcga aaatgtaaaa tgcgacgcgg gaatctttac cgctttttct ttatttttgt
     2641 atcgagccgt tcgaatctac aaaaagtcga tttaagtttc ccgccgaaca attagtcgga
     2701 agcccaggct tcggcgcaag ctatttgaga gaaagtgccg gaaataatac attgaaaaat
     2761 ggaaaactct tctgtcgctg attaagcgct tgtccgggct gatcagcttt tatgaaatgc
     2821 taaaaataag taagggaatt gcctgaatta caattattca ggcaattccc ttacttattt
     2881 tatagagccg caaaaaaatg cggtttagat ttaattaaaa gctaaccagc cgtctgcgcc
     2941 ttatcctcga actctttaat aagagccctg attttttcac catccttggc atccggttga
     3001 gctttcagaa aaagcttcag ctcgcgcgcg gcatcgcgat aacgctttaa atcgtagccg
     3061 tagagcaagg ctagatgcca gttaacgagc ggcattgttc ctttagacaa ttctttagcc
     3121 ttgaccaatt gcttttcgga ttcgtcaaat tgcttatttt gtctctttaa aacgcccgat
     3181 aataaaaacg cttccgccga atattgattc atctcaattg ccttttgaat cgcggctccg
     3241 gcttcggtgt ttttcttaag cgaataaaga gagtaggcga gcccgtacca gctcttatag
     3301 ccgcgcgggt tgacgtcgac cgctaaagtc agaagaattt cagcggcttc gaaatgtccc
     3361 agccggacgt attccgttcc gagtctttca agggcggcat aatatttcgg aaaagcttcg
     3421 atagccgatt tcagattgcc taaaccttcc ttttctttct tatcgcctaa ttccgcgacg
     3481 cctttttcat aaagtttttt ggcgttttcg ggaacttcct gcgcaaatat cgtgccggtt
     3541 acgcctaatt cgcctcttcg gcgcgggcgc agatagaaat ccttctgttc attgctaaaa
     3601 cccgatggtc tgctgtcgcc gtttgatgag cgaacggcga aattaacaat ctcgacttcc
     3661 tgttcctgtt cttcataatc cgtgccggag gaaagaaccc ggatgacaaa gcgtcccgaa
     3721 ccgagcccgg taaatatata tcgaccggag ccattagtgc gcgcgcgccc gataaaacga
     3781 gaatagctgt ccagcaattc gacattgata tcgcctaaag gctcgcgctg agcgccgaaa
     3841 atatgcccgc tgattgtgtt tccgaactgc gcataagtat tagcggaaaa gactggaatc
     3901 agtaccgcca ggctaatcaa taaaaatact ctgcgacgcg ctgcgagtaa attttgcatt
     3961 ttcattacta ctctccttta taacttcagc ttaagctttg tttcttaatt tgacggttta
     4021 tcgacggatt tattcgtcga catttaatag atgtcccagt ttttctcttt taactttcag
     4081 ataatgcgcc gccggctctt ccgattccac ttcgatcggg acgcgttcca gaacctgtag
     4141 accggcttct tccaaagcgc gaattttatc cggattgttt gacatcacgc gcactttaca
     4201 gagacccaaa tcgaaaagaa tctccgcgca ttgctgatac tcgcgcgcgt cgacggcaaa
     4261 gccgagtttt tcgttggctt cgaccgtgtc cgcgccttcg tcctgcaatg cataggcgcg
     4321 aatcttgttg ataatgccga ttccgcgacc ttcctgttgt tgatatacta tcgcgcctct
     4381 accttcggct tcaatcattt ccatcgcgcg ctgcagctgc ggtccgcagt cgcatttgat
     4441 tgagccgaaa acgtcgcccg tcaggcattg cgaatgaatt ctaaccagcg tcggaacatc
     4501 cgccttcatc tcgcctttca ccagcacgac aaattcctcg gagctgttca gcgaacggta
     4561 gccgacaatt ttaaattttc cggtttgcgt cggcaaatct gccgccgcta ctctttccac
     4621 agagagttgc cgaattttcg gcaaacaagt ttcaatgttt cctgtcacgt agatttgcta
     4681 acctcgcaaa taaaattaga aggtttcctt aaattataac tctacggtcg cggaaattat
     4741 aggcttaaag tgattcggca ggatattttg cacaataaat gggcgaattc gtaaaaaaag
     4801 gttagctgtc cggctcttta acaaagccgg acggctaacc ttttcaattt aaagaaaatc
     4861 taggattttt tttcgtcttc gtcttcttct tccggctgct ggtgggcgag caccgcgctg
     4921 tcggaagttc cgaaaatgcg cgaaagaaga ttatttaaga catccgaaac gctggtcggc
     4981 gcattttgcg cgaccggcac gggcgcttcc tcgccggcaa atgcaaaatc gagtctgacc
     5041 agctcagccg ttacctggcg gcaaaacgag cagtcaacca gatgaccgag aatcggcgtc
     5101 gattcgcgct cgcttaaatt accttcgaca aaggctgtca gcgagtcgtc atccagatgt
     5161 ttatcgttac ttataattgc gtctgccgaa cgaacccgca aataattgcc gagtaatccc
     5221 tgaatcatta aatcttccgt cgttgttgtc gttgtcgttt tccccttgtt tatcataatt
     5281 ttccagcctc ccgagagata gacgcaaatt ttcgcggcat ttgcgtaagt ttctcaattg
     5341 gaggctaata agttatttta gacgctcata ttcggcgttt cagttgtcat caaattgcga
     5401 tacagattcg ggctcgccaa gtcctgcgtg gcgattttca tacaaagctc gatttcctcg
     5461 tttttcaaac tgagtccgga gctgagatcg tcgcgaaaca gttttaagat tttcgcgtaa
     5521 gttttttcca gccagcgcat aatggtcgat tcgtgaatcc gcgtcgccgg attctggtcg
     5581 cgcttgtcgg attttctttg aaaccagaac ctgagcggcg aagtcggaac ttcgaccagc
     5641 cgcgcaatct cgcgcaattt taaattttcg acgtgataat aaagcaaaag cagcttttcg
     5701 tgattgtcga gcgtcgaaaa agcgttttcg atcgccgaaa cggtcgcctt gcggtaacgc
     5761 tcgcgcgtca ggtggtcgag catttccgtc tcgccgccga gcacgggttc ggcaaaagtc
     5821 gagtgcgtgt agccttcgcc gaccttttcc gtcatctcgt cgagcgagac ttcgtcgtgg
     5881 ctcgtccggt gaaggtcgac cagcgaatgc cagacgaccg tgcgcagcca gccgcgaagg
     5941 cttccgcgcc cgctgtaagt cgcaaatttt gaaactcttt cgccgtccac gacgcgcgtt
     6001 ccgtaaagct cgacgtagac cgtgtcgata acttcctgcg cgttcatttc cgagctcgcc
     6061 agatgcctcg ccactctttc cagataggcg cgatgctgat ggtcgaattc ccaccacgcg
     6121 cgctcgttgc cgttggcgca ggcaatcgcc ataaacaaat cgcccgcttg aatttcgcgg
     6181 aaaaacgcgc agacttcttc gattgtcggc gtgtttgact cgtcggcaaa aagatatttt
     6241 cgggcggttt tcttcagcga ttccttaaag cattcgagcc ggacgccgaa attcggcgcg
     6301 ttttcatcgc agcgcgcgaa aacttcttcg ataacaggct cgcactgatt cagcagttcg
     6361 tcggtttgat ttgtttcagg tgacatcttg ggaactatga ccgcaattat aaacgaattt
     6421 tgctatttgt catagcaatc ttacgtttgg ctcaaagccg gattcgccaa tccgggcgtt
     6481 tccgccgttc ggtcggcgta ttgcagctgg taaagccgcc agtaaagacc gcgcacggcg
     6541 agcaattcgt gatgcgttcc gctttcgcgc agctcgccgt gatgaaaaac caaaatgcgg
     6601 ttgcagtttt gaatcgtcga aagcctgtgc gccacgacaa acgacgtacg ctcgcgcatc
     6661 acgcgctcga ccgcggcttg aatcagctgc tcggtttccg tgtcgatcga gctggtcgct
     6721 tcgtcgagca caagaatttt cgggtcgaaa gccagcgcgc gcgcaaagct gattaattgt
     6781 ttctgcccga cggaaagtcc tgcgccgcgt tctttcagct cggttttata accgttttcc
     6841 agccgctcga cgaattccgc cgcgtgaact tcgcgcgccg cccagacggt tcgctcgtcg
     6901 gcgatttcct tttttccgag ccgtatattg tcttcaatcg tgccgctgaa aagaaaaacg
     6961 tcctgcaaaa cgaccgcgaa attttcgcgc agcgactgta aatcccaatc gcgcaaatcg
     7021 acgccgtcca gcaggattct gcctttctgc acgtcgtaaa aacgcatcag cagattcgtc
     7081 acggtcgttt tgcccgagcc ggtcgcgccg accagcgcga tcgattcgcc cgctttgaac
     7141 gtaaaggaaa tgtcctttaa aacccaatct tcttttttat aagcgaacca gacgttttga
     7201 aactcgatgt cgccgacggc tttgccggat tttttcgggt tttggggcgt ttcgacctga
     7261 accggcaaat cgaacaaaat aaaaatccgg tgcgaggcga caatcgccgc ctgaaagatg
     7321 ttgaatttat ccgaaaggtc gcgaatcggc tgaaacagca ttcccgaata ttgtatgaaa
     7381 gccgccagca cgccgatggt caaaggctcg ccgcccgccg cgacgaccga taaatattcg
     7441 gcgccgaaag cgaaaatcac cgccgcgatg ccgaccgcgc tgataaaatc cacgagcgga
     7501 taaaaaatcg agtagtaata aatcgtctcg atattcgcgt tgcgataatc gtcgttgatg
     7561 tcgtgaaaac gtttttgcgc cttcgattcc gcgttgaaaa gctggacgct ctgcgcgccg
     7621 gaaatatgtt cctgcaaaaa cgcgttgagc ttcgccgttc gggtgcgaac gcggtcgaat
     7681 ccgcgccgcg cgtgtttgcg aaaccagttg gtcgccgtaa acagaagcgg aaccgtgact
     7741 agcgtgacca gcgccagccg ccaatccatc cagaacatca cgccgacaat ggcgaaaata
     7801 atcaccaaat cgcccagaac gtcaatcacg cccgacgtga aaagctcgtt cagcgcgtcg
     7861 acgtccgatg tgatgcgcgt gataatgcgc ccgaccggat gtttgtcgta ataagaaatt
     7921 tcctgatgtt gcagcttggt gaaaatctcg gtgcgcaaat cgaacatcac gcgctgaccg
     7981 acattgttga ctaaaatctc ctgaaaatag ctgaaaataa accggaaaag aaacagaccg
     8041 aaaaaggcga aggcaaacag ccagatgccg tccgcctgct tcggcacgat aaaatcgtcg
     8101 acggcggact gcgtaaaacg cggctggagg ctgagcagaa tattgctcaa aagcgtcagc
     8161 accagcgcga ccgccgcaat gtgccaatac ggcttcagat aacgcaaaag cctgcgaata
     8221 acgcgcaaat cgtaagtttt accgatttcc tcttcttcgt gaaatttttt cgcttcttcc
     8281 gacataaaat attgttttta gattatctta aatcgggcga gaatcaaatt ttcaattgcc
     8341 aatccggaat ctaaactcta aaatcaaaat caaaatgagc cgctttatcg acgaacgcaa
     8401 aaacaactgg cagaaactgg aaggcttgct ggaaattctg gaaacttcgc ctctgcgcgg
     8461 tctgccgcgc gcgcaggttc gcgagctcgg cgagctttac cggcgcgccg cttcggattt
     8521 ggcgattgcc cgcgccgaat cgcgcgaccc gaagctcgtc aattatctga acagtctcgt
     8581 cattcgcgcg cacggcagga tttaccgcgc cgacacgaag ggcgcgaatc tggtctggca
     8641 atttttcgtc agggattttc cgcaggcgtt tcggcgcagc tggcgctata cggcgctggc
     8701 ttttgcgact tttacgattt tcggcatcgc gtcttttctt ttgtgttatt acgatttgaa
     8761 ttttgccgac gacctcggat taagctcgct gcgctacagc gtgcagtcgg accacaaatg
     8821 gtgggaaagc ttgaacgacg ccaatcaaat cgggtcgagc gaaattttga ccaacaatat
     8881 tatggtcgcc atcagagcct tcgctttcgg cgcgtttttc ggcatcggga cgctttttgt
     8941 tttaatcttc aacggagtgt atatcggcgg cgttctgggc gtttgttatg cgataaatcc
     9001 ggctttcggc aatgcgctgg taacgtttat ggtcgggcac ggcgtgattg agctttcctg
     9061 cattttcatc gcgggcggcg cgggaatgct catcggttat tcgatgatta atccgggaga
     9121 tttatcgcgc acgcaggctt taaagaaaaa cgggatggaa gcggcgcgtc tggcaatcgg
     9181 ctgcgcgtgc ctgctggtcg tcgcgggaat tatcgaaggc tttctctcgc cgtcgctgct
     9241 gcccgcgtgg ataaaatacg gcacgggaat tttaaccgga atcgcgatgt attcttacct
     9301 gtttctggtc ggacgcgaaa aggaagaaaa aagggaagaa aaaatttaac gcctgaagcg
     9361 caaagcgtta gggacgcaaa gggaaaataa ataagcgagt aaaaatcagt aaatcaaatt
     9421 cgtcttttcg cttttacctt ttgtcttaaa acccttacgt tcttaccgct gctattttgc
     9481 gtccccgcgt taaaaatttc tgaaaaggac taagaatgac gaaaaccgaa caagcgagtg
     9541 aaaaaaatcc tgccgaacag cattccgcgc cgtgcgtgat gataatcttc ggcgcgacgg
     9601 gcgatttgac gaaacgcaag ctgtttccgg cgctttacaa tctggcgaaa gacggctatc
     9661 tgcccgaaaa ttttgccatc g
//
LOCUS       DASWNE010000829         4482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_520861, whole genome shotgun sequence.
ACCESSION   DASWNE010000829 DASWNE010000000
VERSION     DASWNE010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520861"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1968)
                     /locus_tag="VGB68_16240"
     CDS             complement(<1..1968)
                     /locus_tag="VGB68_16240"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HEY0050843.1"
                     /translation="MSDKDFQIPESLKDALRSGLVVPFIGAGVSLAVKKRNEDGEKSE
                     QSLFPSWKGFVEILAQALKGETKFNEADYVLSSVNIKKPKYLEAMQHAREELGARLWY
                     KVFEDNFDKSEDEADEDSLKLPKLIWKLGNNLIVTTNVDRVFQWTCPKLAEYRYLDVQ
                     NVEYARLQSEQTPKRPTVWHLHGHLDHKENVIFTFDQYRAFYEERNNEAKLQTLLNFL
                     TDRTFLFIGFSLDDAYIREQLEYVHKIYKGGADSFYILLRQSEIEKANLPGYVTPVPF
                     SDFGKPLEDLIEELSNIANGNSAPPKPPTEPINPKDALKNHFNVPFASKGEAFVGREG
                     LREKIWQSLNQSGRAAIGQAVSIEGFGGLGKTQLAVEFAHAYSDRYENGVFWLTADEN
                     LDNQLIRIGEDLRWISQSDTGFNQAELVRNRFRKLSDCLIIFDNVDEEHPIDNYLPER
                     DARPHILVTSREEQDGFYPINLGILNKEEARILLLQIAERSPKLDAEEKALENILQEL
                     DGLPLAIELVGGYLKRRGSISFQDYYEFLQNEPLERLEEKFSDKTFTKHNKSIIRTLK
                     ISEKLLSETKYLEEILDILAWSGKSTMGYSLLRELVGTQDKLLLKEALGVALELHFIK
                     QEESAERYAIHRLLAKVRRYEKPIEQNQEWHK"
     gene            2188..4155
                     /locus_tag="VGB68_16245"
     CDS             2188..4155
                     /locus_tag="VGB68_16245"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0050844.1"
                     /translation="MFSDNFRRGEGKGKGKTGSKAASKLADIGWKAFQAVNKALPEGE
                     SIKPEWAAEPLLKSYERTSPPLGFPRETDSLCPTCVKQVREGVTSGSIPLEILMNSHP
                     GEIKAQILEENGQVLMRKTCPTHGEFEDVLATDAKFLDRIEKLFYGRDFKAAEDKHVH
                     HHGTSDIKFGRGAVLTVDLTNRCNMMCNPCFMDANQVGYVHEPTFEDTKAILDRAISF
                     KPRRQIIILFSGGEPTIAPHFLESVAYAKKIGFYRILAATNGIRYAEDIEFCKAAKEA
                     GQHGVYLQFDGVSEEKNKHRGVGNLFDVKLKAIENLASVGIKVTLVTTIVNTVNNDGI
                     GDIVKFAAENIDKVQTIAFQPVSFTGRDEDISDADRKQQRYTLAGMTHDLNKQLEGSL
                     DTQPLRDWFPLSSYSAFTSVMDMLQGADAPWGWSSCNCHPNCGIFTLVVVNKQTGAMK
                     SLFEFFNYEQFMKDVAVITDTARGRKLTMAQLGMAIMRNYNASNAPEGFPISQILNLF
                     KPSSANSNSDRNDRMKAQPKDESEDVWRVLCVEGMWFQDLFTYDFRRTEMCVIPYGTQ
                     EGEISFCAYNTGVGWRQIIEEMHKTASLSEWYKEHSRHAVYAKGKEVPGIKKTRSLSL
                     PVIAKIVSEDLDAPSVSPYLVEEEENETVGV"
     gene            4190..4399
                     /locus_tag="VGB68_16250"
     CDS             4190..4399
                     /locus_tag="VGB68_16250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050845.1"
                     /translation="MPDERDVANDKRVLQQNEREGTTAEMEGRHPEHAPQPGQKTPQE
                     HEDKNAIDESGNLLHPKEKDENDGM"
BASE COUNT         1168 a         1067 c         1118 g         1129 t
ORIGIN      
        1 cttatgccac tcctgatttt gttcaatcgg tttttcgtaa cggcgaactt tggctaatag
       61 acggtgaatt gcataacgtt ccgcgctttc ttcctgttta ataaaatgca attctaaagc
      121 cacgcccaga gcttctttta ataaaagctt gtcttgtgtt ccaactaatt cgcgcaaaag
      181 tgagtaaccc attgtagatt ttccactcca cgctaaaata tctaaaattt cttccagata
      241 tttggtttca ctaagaagtt tttcgctaat ctttagagtg cggataatac tcttattatg
      301 tttggtaaaa gttttgtcgg aaaatttttc ttcgagtcgt tccagcggct cattttgcag
      361 aaattcatag taatcctgaa aagaaatgct gccacgacgc tttagatagc cgccgacgag
      421 ttcgatagct aaaggcagac cgtcgagttc ctgtaaaatg ttttccagcg ctttttcttc
      481 cgcgtccagt ttcggagaac gctcggcaat ttgaagcaat agtattcgtg cttcctcttt
      541 gtttaaaata cctaaattta tcggataaaa accatcttgc tcttcacgcg aagtcaccaa
      601 aatatgcgga cgagcatctc tttcaggcag ataattatca ataggatgct cttcatcaac
      661 gttatcaaaa ataatcaaac agtcggaaag tttgcgaaaa cggtttcgca ccagttcagc
      721 ttgattaaaa cccgtatcgg attggctgat ccaacgcaag tcttcaccaa ttctaattag
      781 ctgattgtcg aggttttcgt cagccgtcag ccaaaacact ccgttttcat aacggtcgct
      841 ataagcgtga gcaaattcga cagcaagttg ggttttaccc aaaccgccaa aaccttcaat
      901 actgacggct tgaccgatag cagcgcgtcc gctttgattg agcgattgcc aaatcttttc
      961 gcgcaagcct tcgcgcccta cgaaagcttc gcctttggag gcgaacggca cgttgaaatg
     1021 gtttttcagc gcgtctttcg gattaatcgg ttcggttggc ggcttcggcg gcgcgctgtt
     1081 gccgtttgcg atattggata attcttcaat taaatcttcc agaggtttgc cgaaatcgga
     1141 aaagggaacg ggcgtgacgt atccgggcag attcgctttt tctatctcgc tttggcgaag
     1201 cagaatataa aaggaatccg cgccgccctt gtaaattttg tgaacgtatt cgagttgctc
     1261 gcgaatgtaa gcgtcgtcga ggctgaaacc gataaaaaga aacgtgcggt cggttaaaaa
     1321 attaagcaga gtctgaagtt tcgcttcgtt gtttctttct tcgtaaaagg ctctgtattg
     1381 gtcgaacgtg aaaattacgt tttccttgtg gtcgagatga ccgtgcagat gccagaccgt
     1441 cggacgtttc ggcgtctgct cgctttgcag ccgcgcgtat tcgacattct gcacgtcgag
     1501 gtatctgtat tccgcgagtt tcgggcaagt ccattgaaaa acgcggtcaa catttgtcgt
     1561 gacaatcaga ttattgccga gtttccagat aagttttgga agcttcaaag aatcctcgtc
     1621 ggcttcatct tcggatttat caaaattatc ttcaaagact ttataccaga gccgtgcgcc
     1681 gagttcttcc cgcgcgtgct gcatcgcttc gagatatttc ggctttttga tgtttacgct
     1741 ggaaagaacg taatcggctt cattaaattt ggtttcgcct tttaatgctt gagcgagaat
     1801 ttcgacaaaa cctttccagc tcggaaaaag agattgttcg gatttttcgc cgtcctcgtt
     1861 ccttttcttt acggcaagcg agacgcctgc gccgataaag ggaacaacca gtccgctccg
     1921 aagcgcatct ttaagagatt cagggatttg gaaatctttg tccgacatga gagccttgcc
     1981 cgagtttgtt ctcaataaaa cgcattgagc cggtcgtgat aaaagcgtta acggcgaaaa
     2041 actgtcgtca ttatgcgtca atttttgtaa agtttccagc atttgcgcgg gcgaaaattg
     2101 gcaaacgggc tgaaatttgt tagcctttag cttggctttt tgtgtctcta tcttaaaaat
     2161 tctatttaaa ctgttacaat aaaatctatg tttagtgata attttcgtcg cggcgaagga
     2221 aaagggaagg ggaaaaccgg ctcgaaagca gcatcgaagc ttgccgatat cggctggaag
     2281 gcgtttcagg cggtgaacaa ggctctgccg gaaggcgagt cgattaagcc cgagtgggcg
     2341 gctgaaccgc ttttgaaatc gtacgagcgc acgtcgccgc cgctcggttt cccgcgcgaa
     2401 accgattctt tgtgcccgac ctgcgtcaag caggtgcgcg agggcgtaac gagcggcagc
     2461 attccgctcg aaattttgat gaactcgcat ccgggcgaaa tcaaggcgca gattctggaa
     2521 gaaaacgggc aggtgctgat gcggaaaacc tgtccgacgc acggcgaatt tgaagacgtt
     2581 ctggcgacgg acgcgaagtt tttagaccgc atcgaaaaat tgttttacgg gcgcgatttc
     2641 aaagccgccg aagacaagca cgtgcatcat cacggcactt ccgatataaa attcgggcgc
     2701 ggcgcggtgc tgaccgtcga tttgacgaat cgctgcaata tgatgtgcaa cccgtgtttt
     2761 atggacgcca accaggtcgg ctacgtccac gagccgacgt ttgaagacac gaaagctatc
     2821 ttggaccgcg cgatttcgtt caagccgcgt cgtcaaatca tcattttatt ttcgggcggc
     2881 gagccgacga ttgcgccgca ttttctggaa tcggtcgcct acgctaaaaa gattggcttt
     2941 taccgcattt tagccgcgac caacggcatt cgctacgccg aggacataga gttttgcaag
     3001 gcggcgaaag aagccggtca acacggcgtt tacctgcaat tcgacggcgt ttcggaagag
     3061 aaaaacaagc atcgcggcgt cggcaatttg ttcgacgtga aattaaaagc gattgaaaat
     3121 ctcgcgtcgg tcggcattaa agtgacgctg gtgacgacga tcgtcaacac cgtcaacaac
     3181 gacggcatcg gcgatatcgt gaaatttgcc gccgaaaaca tcgacaaagt tcagacgatc
     3241 gccttccagc cggtttcttt tacgggacgc gacgaggaca tttcggacgc cgaccgcaaa
     3301 cagcagcgct acacgctggc gggaatgacg cacgatttga ataagcagct cgagggaagt
     3361 ctcgatacgc agccgctgcg cgattggttt cctttaagct cttattcggc ttttacttcc
     3421 gtgatggata tgcttcaggg cgcggacgcg ccgtggggct ggtcgtcgtg caattgccac
     3481 ccgaactgcg gaatctttac gctcgtcgtc gttaacaaac agacgggcgc gatgaaatct
     3541 ctgttcgagt ttttcaacta cgagcagttt atgaaagacg tcgccgtcat caccgacacg
     3601 gcgcgcggca ggaaattgac gatggcgcag ctcggaatgg cgattatgcg caactacaac
     3661 gcatcgaacg cgccggaagg cttcccgatt tcgcagattt tgaatctttt caaaccttcg
     3721 tcggcaaact cgaactccga ccgcaacgac cgtatgaaag cgcagccgaa agacgaatcg
     3781 gaagacgtct ggcgcgttct gtgcgtcgag ggaatgtggt ttcaagacct tttcacatac
     3841 gatttccgcc gcacggaaat gtgcgtcatt ccctacggca cgcaggaagg cgaaatcagc
     3901 ttctgcgctt ataacacggg cgtcggctgg cggcaaatca tcgaggaaat gcacaaaacc
     3961 gcctctttat ccgaatggta taaagaacac agtcgtcacg cggtttacgc caagggcaag
     4021 gaagttccgg gcatcaagaa aacccgcagc ctgagcctgc cggtgattgc caaaatcgtt
     4081 tccgaagacc tcgatgcgcc gagcgtttcg ccttatctgg tggaagagga agaaaacgaa
     4141 acagtcggag tttaatcaaa cacagaacga atgacgcaga tataaaatta tgccggacga
     4201 aagagatgtc gctaacgaca aaagggtttt gcagcaaaac gaaagagaag gaacgaccgc
     4261 cgaaatggaa ggcaggcatc ccgaacacgc gccgcagccc ggacagaaaa cgccgcagga
     4321 acacgaagat aaaaacgcga ttgacgaaag cggcaatctg cttcatccaa aggaaaagga
     4381 cgaaaacgac ggaatgtaat tcgtccgcga attaaaaaag ccttgaggtt ttgcaaaacc
     4441 tcaaggcttt tttaattcgc ggacgaatta cattccgtcg tt
//
LOCUS       DASWNE010000830         4453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_339326, whole genome shotgun sequence.
ACCESSION   DASWNE010000830 DASWNE010000000
VERSION     DASWNE010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339326"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VGB68_16255"
     CDS             <1..267
                     /locus_tag="VGB68_16255"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0050846.1"
                     /translation="ESPNLVLMDVAIPVVDGLEATRRIREFAASDVMPVVFISGYTQN
                     SLRLMALDSGGNDYLIKPIDFKELEKMIDKHIKKTGATYSLNDL"
     gene            344..1207
                     /locus_tag="VGB68_16260"
     CDS             344..1207
                     /locus_tag="VGB68_16260"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY0050847.1"
                     /translation="MTIKKLATNESNNINGFAYKASLSKLQNRELPSRRRALSGRLMK
                     RRGQSAPEASQIKKSFSNQLLNSLTEGDYARLSPYLETVSLSSGEELNKPGESINYVY
                     FPEDMTVSQFQGFADGSTAEIIMVGNEGMVGLNAVFASTPSPFWTQVMTEGKVFRIKT
                     AVLKDEFLHNPSLQFLLLNYANTYIAQISQRVSCNIRHVAEARFCTWLLMLQDRTASE
                     CLRLTQEQIAVCLGINRPTVTNLAQSLKENGIIDYVRGCIKILDRARLENSACECYQA
                     TKEYLQFSVLH"
     gene            1481..2674
                     /locus_tag="VGB68_16265"
     CDS             1481..2674
                     /locus_tag="VGB68_16265"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY0050848.1"
                     /translation="MTDNLTETPDIPNYDLVCLSHLRWDFVYQRPQHLLSRFARERRV
                     FFIEEPIFSDEPTHLAVSPREDNLSVVVPYLNHADAADNTIENLIRRLLDEMFDERSI
                     KDFVLWYYTPMAVGFTDHLQPQAIVYDCMDELSMFKNAPPQLIKNEAKLFEKADVVFT
                     GGQSLYEAKRHQNPNVWAFPSSIDTAHFNKARKIAGEEMEDQKAIPHPRLGYAGVIDE
                     RIDIDILREIAEKRPEWQIVMIGPVVKIDEADLPRPGNIHYLGMKDYQQLPSYIAGWD
                     VALMPFALNDSTRFISPTKTPEYLAAGKPVVSTAIRDVVRPYGEQNLVAIAKNADEFI
                     EAAQQAMNENRAEKLLAVDKFLLQTSWDKTWRRMVDLIAESIAENRAKAAEKERFGRK
                     TTAGT"
     gene            2739..3938
                     /gene="glf"
                     /locus_tag="VGB68_16270"
     CDS             2739..3938
                     /gene="glf"
                     /locus_tag="VGB68_16270"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HEY0050849.1"
                     /translation="MFDYLIVGAGFAGSVLAERLANGSGKKVLICDKRPHIAGNAYDC
                     YNNDGILIHKYGPHIFHTNSRDVFDYLSRFTEWRHYEHRVLASVDGMLLPIPINLDTI
                     NKLYGLNLTSFEMVDFLKNLGEKRDHIRTSEDVVVSTVGQELYEKFFRNYTRKQWGLD
                     PSELDASVTARVPTRTTRDDRYFTDSYQAMPKYGYTRMFENLLDSPNIKIALNCDYRE
                     LLEDVKYKEMIYTGPVDEFFDYRYGKLPYRSLEFKHETHDAETFQPAPVVNYPNEQAF
                     TRVTEFKYLTGQEHKKTSIVYEYPQAEGDPYYPVPRKENAEIYNKYKLLADARDDVHF
                     VGRLATYKYYNMDQIVAQALTQYGKIVGVKRQQAAQLARPAKAGASSIYIPKVTGNGN
                     GKAVTEF"
     gene            complement(4149..>4453)
                     /locus_tag="VGB68_16275"
     CDS             complement(4149..>4453)
                     /locus_tag="VGB68_16275"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY0050850.1"
                     /translation="TLRDVKSASRHDLGAVCFADGKIFKANDFANLEVLDRVGSGDAF
                     AAGLIYGLLAGKDFEYAVNAGAASSALTMTTLGDGSSATLAEVEKLVHSRELNLSR"
BASE COUNT         1207 a         1088 c         1098 g         1060 t
ORIGIN      
        1 gaatctccga atctggtttt aatggatgtg gcgattccgg tcgtcgacgg tctggaagcc
       61 acgcgccgca tccgcgagtt tgccgcatcg gacgtgatgc ccgttgtttt catttccggt
      121 tacacgcaga acagccttcg cctgatggcg ctcgattccg gcggaaacga ttatctgatt
      181 aagccgattg atttcaaaga actggagaag atgattgaca aacatattaa aaaaacaggt
      241 gcgacttata gtttgaatga cctgtaacgc gcgcgggcgg cgtcggcatt atgtagtttt
      301 taagcgtaaa agcgattgaa ttttatcaag gcaggtgggg ttaatgacaa taaaaaaatt
      361 ggcgacaaat gaatcgaata atataaacgg ctttgcttat aaagcttccc tttcaaaact
      421 tcagaacaga gaattgccga gccgccgccg cgctctgagc ggcaggctga tgaaaagacg
      481 cgggcaatcg gcgccggagg catcgcaaat caaaaaatca ttttccaatc agctgcttaa
      541 ctctctgacg gaaggcgatt acgcgcgcct ttcgccgtat ctggaaaccg tctcgctttc
      601 gagcggcgaa gaattaaata aaccgggcga gagtataaat tacgtctact ttcccgaaga
      661 tatgacggtt tcgcagtttc agggttttgc cgacggcagc acggctgaga ttattatggt
      721 cggcaatgaa ggtatggtcg gcttaaatgc cgttttcgcc tcgacgccgt cgcctttctg
      781 gacgcaggtg atgaccgagg ggaaagtctt ccgcattaaa accgccgtgc tcaaagacga
      841 atttttgcat aacccgagcc tgcaattttt actgctgaat tacgccaaca cttatatcgc
      901 gcagatttcg cagcgcgttt cctgcaatat ccgccacgtt gccgaagcgc gtttctgcac
      961 ctggctgctg atgctgcagg accgcaccgc aagcgaatgc ctgcggctga cgcaggaaca
     1021 gattgccgtc tgtctgggaa tcaatcgccc gaccgtcacg aacctggcgc agtcgctcaa
     1081 agaaaacggc attatcgact acgttcgcgg ctgtatcaaa atcctcgacc gcgcgcggct
     1141 ggaaaactca gcgtgcgaat gctaccaggc gacgaaggaa tatttgcagt tttccgtttt
     1201 gcattaattc gaaaatgaag gatggaaagg tttcgaattt tgaattttga ataattcgct
     1261 ttatcgcaaa ctcaaaattc gaaacctgaa actttttgtc cttcatccct agtattggcg
     1321 gcataataaa cataaaaatt taataatgtc agctaacgta catacaattt gcgcctcgca
     1381 atgatagact aaatttatat ataatttaga gaatatttta tcagccggct aatttcggca
     1441 aggttatttt tgaaaaataa tgaacaaagg gaatgatttt ttgacagata atttaacgga
     1501 gacgcccgat attccaaact atgatttagt ttgtctctcg catttacgct gggatttcgt
     1561 ctatcagcga ccgcagcact tattgagccg tttcgcccgc gaaagacgcg tctttttcat
     1621 cgaagaaccg attttttcgg acgagccgac gcatctcgcc gtcagcccgc gcgaagataa
     1681 tctatcggtc gtcgtgcctt atctgaatca cgccgacgcc gcagataaca caatcgaaaa
     1741 tttaatccgc cggctgctgg acgaaatgtt cgacgagcgc tcgattaaag atttcgtttt
     1801 gtggtattac acgccgatgg cggtcggctt taccgaccat ttgcagccgc aggcgattgt
     1861 ttacgactgt atggacgaat tgtcgatgtt taaaaacgcg ccgccgcagt tgataaaaaa
     1921 cgaagcgaaa ttgtttgaaa aagccgacgt cgtttttacc ggcggtcaga gcctttacga
     1981 ggcaaagcgt caccagaatc cgaacgtctg ggcttttccg tcgagcatcg acacggcgca
     2041 tttcaacaaa gcgcgcaaga ttgccggaga ggaaatggaa gaccagaaag cgattccgca
     2101 tccgcgcctc ggctacgcgg gcgtgattga cgagcgcatc gacatcgaca ttttgcgcga
     2161 aatcgccgaa aagcgtcccg aatggcagat tgtgatgatt ggtccggtcg tcaaaatcga
     2221 cgaagcggat ttgccgcgtc ccggaaatat tcattatctg ggaatgaaag attaccagca
     2281 gctgccgtcg tatatcgccg gttgggacgt cgcgctgatg ccgtttgcgc tgaatgattc
     2341 gacgcgcttt atcagcccga ccaagacgcc cgaatatctc gccgccggaa aacccgtcgt
     2401 ctccaccgcg attcgagacg tcgtgcgacc ctacggcgag cagaatctgg tcgccatcgc
     2461 caaaaatgcc gacgaattta tcgaagcggc acagcaggcg atgaatgaaa accgtgcaga
     2521 gaagcttctg gcggtcgata aatttctgct gcaaacttcg tgggataaaa cgtggcggcg
     2581 aatggtggat ttgattgccg aatccatcgc cgaaaatcgc gcgaaggcgg cggaaaaaga
     2641 gcgtttcggc agaaaaacga cggctggaac gtaaattgct aaacataggt ttacgctcta
     2701 tatattgtct taaaaacgaa caggggaaag gtaataaaat gtttgactat ctaattgtcg
     2761 gagccgggtt tgccggcagt gtcttggcgg agcgtcttgc aaacggctcg ggaaaaaagg
     2821 ttttgatttg tgacaagcgc ccgcatatcg ccggaaatgc ttacgattgt tataacaacg
     2881 acggcattct gattcacaaa tacggaccgc atatttttca caccaattcg cgcgatgtct
     2941 ttgattatct atcgcgtttt accgaatggc gacattacga gcaccgcgtt ttagcgtcgg
     3001 tcgacggaat gcttctgccg attccgatta atctcgacac gattaataaa ctttacgggc
     3061 tgaatctgac ctcgtttgag atggtcgatt ttctgaaaaa cctcggcgaa aaacgcgacc
     3121 acatcagaac ttcggaagac gtggtggttt ccacggtcgg gcaggagctt tacgaaaagt
     3181 ttttccgcaa ttacacgcgc aagcagtggg gcttagaccc gtcggagctc gacgcttcgg
     3241 tgaccgcccg cgtgccgacg cgcacgacgc gcgacgaccg ttattttacc gattcttacc
     3301 aggcaatgcc gaaatacggc tacacgcgga tgtttgaaaa tctgctcgac agcccgaaca
     3361 tcaaaatcgc gcttaactgc gattaccgcg agctgctcga agatgtcaaa tataaggaaa
     3421 tgatttacac cggaccggtc gatgaatttt tcgattatcg ttacggaaaa ttgccgtatc
     3481 gctcgctgga attcaaacac gaaacgcacg acgccgaaac tttccagccc gcgcccgttg
     3541 tgaattaccc gaatgagcag gcttttacca gagtcaccga attcaagtat ttaaccggtc
     3601 aggagcacaa aaaaacgagc atcgtttacg aatatccgca ggcggaaggc gacccgtatt
     3661 atccggttcc gcgcaaggaa aacgccgaga tttataataa atacaagctg ctcgccgatg
     3721 cgcgcgacga cgtgcatttc gtcggacgac tggcaactta caaatattac aatatggacc
     3781 agattgtcgc gcaggcgctg acgcaatacg gcaaaatcgt cggcgtcaaa cgccagcagg
     3841 cggctcaact tgcccgaccg gcaaaagccg gcgccagctc aatctatatt ccgaaagtaa
     3901 ccggcaacgg caacggtaaa gcagtaaccg aattttagaa atagagcggt aaaaaaataa
     3961 aagcacgaga tgatttaatt atcatctcgt gcttttttaa tatgaattcc gaaaactatt
     4021 tttatcttta agacaaaaga acacaacgag gaaaaacgcg cggaaatgac cggacgaatt
     4081 gatgatatat aaaaatcgtc tttcacgttg ctgtcagctc ttttttgtct gtttgcggcg
     4141 aatttccatt aacgggaaag attcagctcc cggctgtgaa ctaatttttc cacttccgcg
     4201 agcgtcgccg acgaaccgtc gccgagcgtc gtcattgtca atgccgaact ggcagcgccc
     4261 gcgttgaccg cgtattcaaa atctttaccg gctaacaaac cgtaaatcaa accggcggca
     4321 aaagcatcgc cgctgccgac gcggtcgagc acttccaaat tcgcaaaatc gtttgcttta
     4381 aaaattttgc cgtcggcaaa acaaaccgcg cccaaatcgt gtcgcgatgc cgatttcacg
     4441 tcgcgcaagg ttg
//
LOCUS       DASWNE010000831        11284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_559496, whole genome shotgun sequence.
ACCESSION   DASWNE010000831 DASWNE010000000
VERSION     DASWNE010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11284
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559496"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..925
                     /locus_tag="VGB68_16280"
     CDS             29..925
                     /locus_tag="VGB68_16280"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEY0050851.1"
                     /translation="MKNQNWFAVEITVDAEAAEAVEFAFNELGSAGTEINNLRKNAGA
                     PVCVAGYFNEKLDAGIVRSQLDEALRIYGFSPDAVHKIEPREIEDTDWLSEWKKHWRP
                     TETARFIIAPTWEKPENPDKFVIYIEPSMAFGTGTHETTRLCLKAIEEDFAGEMSFLD
                     VGTGTGILAIAAAKLKIQNSKFKILACDTDEDSIKIARENAELNGAAKEIEFYVGSIS
                     EKTPEFDFVCANLTADVIIPLLPLLAKKTKRILVLSGILKEQENLIVSELKNVEISDF
                     KIETDGEWISVLAENKKRKTEN"
     gene            complement(954..2981)
                     /locus_tag="VGB68_16285"
     CDS             complement(954..2981)
                     /locus_tag="VGB68_16285"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEY0050852.1"
                     /translation="MKLKKNTIGRGLAGFFNRGFSASFFGFFKAFCVLCVLCGSITAQ
                     KQNALGWVWQNPLPQGNPLYSIGFAKDRETGFAVGADGTILSTLDGGFSWKSQIPPIN
                     TIFFGVYVKDKLRAVAVGARGVILLTDNGGKDWREIKSPIKDHLYSVTFAGEGQQNGW
                     AVGTYGRILRTSNGGLTWQEQISNTSEHLVKISFFNQTDGAAVGANGVILTTKNGGFT
                     WKVNTPCPELAMTNVVFLSKEKLIAVGYGGCVVRSDDAGETWSKINVFTRADFLALAF
                     TDEKNGWMTDSNGMVWLTGDGGSTWLPFNVRTRPKLVSLHFSDNFTGWSVGENGLIIR
                     TDDGGYSWQRLSEGGRDDVNDLTFYNENLGWSVGSRGRIWLTNDGGQNWLPVFSDVEA
                     NLNAVYFLNDERGFIVGDKGTVLRTINGGASWGKGIAGATEDLSGVAFINEKTGFAVG
                     ANGTILRSDSAGAYWEYVDSGVKVWLEDIKFTDARHGFIVGDKGTVLRTEDGGLTWQK
                     VKTNLRENLYAISFFDEKTGFTVGANGTILSTVDGGATWIDQETNLQMNLYAVSVFRK
                     NEAIAVGEQGAVLRTLDGGKTWEIQPNVTSKSLQAVFYRGSTDLWVAGRGGAILKRSQ
                     TLSTVKTTSSQKAPPILRGSTRPRPRLKTPILTITDDGDIPPAVPPKKENQ"
     gene            complement(3021..3479)
                     /locus_tag="VGB68_16290"
     CDS             complement(3021..3479)
                     /locus_tag="VGB68_16290"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase H family protein"
                     /protein_id="HEY0050853.1"
                     /translation="MKEVTIVCDGSSLGNGKGTTRAAAVAVLGYRGFWRAFGEYLGNA
                     TNQQAEIAAAWVGLKTLREPCKVKVLTDSRYVVETMTGNFRKKTNLEWWAKLDKEASR
                     HQIKWEWVKGHAGHEVQEVADSMARRMAELGRVDEAILDEAAAEIGVKEI"
     gene            complement(3790..6471)
                     /locus_tag="VGB68_16295"
     CDS             complement(3790..6471)
                     /locus_tag="VGB68_16295"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEY0050854.1"
                     /translation="MHITRVELENIKSHAQATFEFQRGTTAITGENGAGKTTIIEAIA
                     WTLFDLLDYKKDDFARRGAKTKGIARVTFESSLDEREYVIYRDTANGYYVFDPQLKTR
                     IAEKKEEVSRFLWQHLGVEAGTDLETLFRRAIGVPQGTFTAIFMETATERKKAFDKLL
                     KVEEYRQGAEKLRETVNFVRNKITDAREKIARAEGELMRLETVENEHQAVAGQIENLS
                     GSIEKLNAEAAERRAAVKSLDEIETKVNELKTRRDRLQSELATAEVVRGQKETETNQA
                     RAASEKLKLVEADYQTHVSALAELKNLDAQRVERDRIRNQLTKIENEIIKTQAERKNL
                     NENLAKIVEAKEKIVSLQPQTVEQNELEARREQLIKEKAGAAAFEKTIASLEEKISRL
                     RESFRENNAKLKEAETKTNDARQVEILQKRDSDLMSRLAEFRADLARDEKFQSEIRNG
                     LCPILSEKCLNLKPGESLEGFIKTQFSETKKQIITLEVEQKEVFEALRTAREAEKFLG
                     GLEAYRQREREIKEEGIRLKTEQDALKAHVENLPSLEKQIAETESRLKSLDNPKARVL
                     AFEREARRETEFTENLSKIQRNLARLETEKSGFGEQLSKFEALDAEWKRFSDERERTA
                     AAHREFLTNEALAKTLAARETEFAEATGKAAQLKIGLENASGEFESASQTYDRERHLS
                     EKAALLEAEKRLAETRATFEAAKRRESELSAELRRLKVIRVTMQAEFQEKERLEKIHE
                     ATDFIRETLKEAAPRVARNYVYHVSLEANQMYREITGNAERTLKWTEDYGIVLEENGY
                     DRPFSNLSGGEQMAAALSVRLALLKQLSDVRLAFFDEPTTNMDAARRENLATQIGNIR
                     HFDQLFVISHDDTFEGYVDNVISISAEADENPAATEE"
     gene            complement(6598..7554)
                     /locus_tag="VGB68_16300"
     CDS             complement(6598..7554)
                     /locus_tag="VGB68_16300"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEY0050855.1"
                     /translation="MFFPLLTKSPAKAAEFIRRGGLSAFPTETVYGLGADVFDERAVE
                     KIFIAKGRPPDNPLIAHVGNLEQIKLLASEITPAAQEFIEAFFPSPLTLVLPKAKKVP
                     LAATANLQTVGVRMPNSPLALEFLKACATPVVAPSANLSGKPSPTTWQAVFEDLNGRI
                     DCILQGEMTEIGLESTVVDCTCDVPLVLRAGAVSLEDLQKIVPETRTYRVKENEAVRS
                     PGLKHRHYSPRARVILVNSGFGIQDSGFQISDSSAFIGLNKPAQNFDLTKICRSVEEY
                     AHEVFNFFRECDRRAVEIIYCQTVEEKGIGAALMDRLKRSSQ"
     gene            7748..8179
                     /locus_tag="VGB68_16305"
     CDS             7748..8179
                     /locus_tag="VGB68_16305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050856.1"
                     /translation="MKITLTVLLILFFNLILQSGSGCGGEGGNGGGSQCPTPFYPPYD
                     VRVRFDPYQGGIVVSWNERGYKDGYQVFRCVYNCVQVGQDGKYYANLGSFEAITNNRF
                     AGTFYVDKNLSHGTTYHYAIQNRYQSCGVNWSTNYATITAP"
     gene            complement(8311..8574)
                     /locus_tag="VGB68_16310"
     CDS             complement(8311..8574)
                     /locus_tag="VGB68_16310"
                     /inference="COORDINATES: protein motif:HMM:NF039182.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5654 family protein"
                     /protein_id="HEY0050857.1"
                     /translation="MARETTRMILQSMIALSSAALGLVAALAWNDAIKETIKQIMDGD
                     ESLASKYTYAILATIIAVIVVLVLARVAAKVGGEAAIAREAEG"
     gene            8702..10540
                     /gene="uvrC"
                     /locus_tag="VGB68_16315"
     CDS             8702..10540
                     /gene="uvrC"
                     /locus_tag="VGB68_16315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEY0050858.1"
                     /translation="MTLEEKLKNLPVAAGVYLHKNADGKIIYVGKAKNLRNRVRQYFQ
                     SSRNQDRKTRELVRRITDFEFIVVDSEVEALVLESNLIKKHKPRFNVLLKDDKQYPHL
                     KMTNEPFPKVVITRKILRDGASYYGPFLPANLARKTLDLINRTFQLRTCDIEIDGKLP
                     RPCLEYHLKRCLAPCVKELCKPDEYAQAASDVKLLLEGKNKELADALQQRMWHFAETE
                     NYELAAKYRDLHKTVLALGEQQKMATTAERDVDIFGFYREGTRLALQLFTMREGKIVG
                     RREFFWEDLDADNFSISDFLSEVLSQYYSTDYVPLEINVPADFEDREILEKALTERRG
                     RKVRILDPKRGQKREMIALVENNAKIAFEQRFRVLQPAAQKLLEDLMEILELPHFPER
                     IESFDISNISGAENVAGIVVYENGKPNRAEFRRLIIKSVEGANDFASMNEAVFRRYKR
                     LLNEDKPLPQLVFIDGGKGQLTAAAAAMQALDLEGVITLVGLVKPPKRHNEISHLLRF
                     GSEDRPIYFEMGVPAFRLVQQIRDETHRTAIQYHRKRREMRDFTSELTAIPGIGDKRK
                     LKLLRNFGSLEKVAQASAEQLAPFVGAKAAQEIFEHFERQRKLAEN"
     gene            complement(10614..10799)
                     /locus_tag="VGB68_16320"
     CDS             complement(10614..10799)
                     /locus_tag="VGB68_16320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050859.1"
                     /translation="MFVEQVEMFVERAETVVEWLEGCFVGMSKTVGWTLSATGGARFL
                     KRSAALKMRVDGFVMRD"
BASE COUNT         2579 a         3146 c         2784 g         2775 t
ORIGIN      
        1 tttcgccgag gtggattatt agcgcgcaat gaaaaatcaa aactggtttg cagtcgaaat
       61 cacggttgac gcagaagcgg cggaagccgt cgagtttgcc ttcaacgaac tcggttccgc
      121 cggaacggaa atcaataatc tgcggaaaaa cgccggcgcg cccgtctgcg tcgccggtta
      181 tttcaacgaa aaactggacg ccggaatcgt tcggtcgcag ttagacgaag ccttgcggat
      241 ttacggcttt tcgcccgacg ccgttcataa aatcgagccc cgcgaaatcg aagataccga
      301 ctggctttcc gaatggaaaa aacactggcg accgaccgaa accgcgagat tcattatcgc
      361 gccgacctgg gaaaagccgg aaaaccccga caaattcgtg atttacatcg agccttcgat
      421 ggctttcgga acaggcacgc acgaaacgac gcggctctgc ctgaaagcga ttgaagaaga
      481 ttttgcgggc gagatgagct ttctcgacgt cggcacggga accggcattc tggcgattgc
      541 cgccgcgaaa ctgaaaattc aaaattcaaa attcaaaatt ctcgcctgcg atacggacga
      601 agattcgata aaaatcgccc gtgaaaacgc cgagcttaac ggcgcggcaa aagaaatcga
      661 gttctacgtc ggctcgattt ccgaaaaaac tcccgaattt gatttcgtct gcgcgaactt
      721 aaccgccgac gtcatcattc cgcttttgcc tttgttggct aaaaaaacga aacgcattct
      781 ggttttgtcg ggaatcttga aggaacagga aaatttaatc gtttccgaat taaaaaatgt
      841 cgagatttca gatttcaaaa tcgaaaccga cggcgaatgg atttcggttc tggcagaaaa
      901 caaaaaacgg aaaacggaaa actaaaattt tcttcatttc catttcccgt ttttcactga
      961 ttttctttct tcggcggaac ggcgggcgga atatcgccgt cgtcggtaat ggttaaaatc
     1021 ggcgttttca gtctcggtct ggggcgcgtc gagcctctta aaatcggcgg cgctttctgc
     1081 gacgaagtgg ttttgaccgt cgaaagcgtt tgcgagcgct tcagaatcgc gccgccgcgt
     1141 ccggctaccc agaggtctgt gctgccgcga taaaacaccg cctgcaaaga tttggaagta
     1201 acattcggct gaatctccca ggtttttccg ccgtccagcg tccgcaaaac cgcgccctgc
     1261 tcgccgacgg cgatcgcctc gtttttccga aaaaccgaca cggcgtaaag attcatctgc
     1321 aaattcgttt cctggtcaat ccacgtcgcg ccgccgtcaa cggtggacaa aatcgtgccg
     1381 ttcgcgccga cggtaaatcc ggttttctcg tcaaaaaaac tgatggcgta aaggttttcc
     1441 cgcagatttg ttttgacctt ctgccacgtc aaaccgccgt cttcggtgcg cagaaccgtg
     1501 cccttgtcgc cgacgataaa accgtgtctg gcgtcggtaa attttatgtc ttcgagccag
     1561 actttgacgc ccgaatcgac atattcccag taggcgcccg ccgaatcgga acgcaggata
     1621 gtcccgtttg ctccgaccgc aaaacccgtt ttttcgttga taaaagcgac gcccgacaaa
     1681 tcttccgtcg ccccggcgat gcctttgccc cagctggcgc cgccgttgat ggtgcgcaga
     1741 accgtgccct tgtcgccgac gataaaaccg cgctcgtcgt tcaaaaaata cacggcgttc
     1801 agattggctt ccacgtccga aaataccggc agccagtttt gcccgccgtc attcgtcagc
     1861 cagattctgc cgcgcgagcc gaccgaccag ccgagatttt cgttgtaaaa agtcaaatcg
     1921 ttgacgtcgt cgcgaccgcc ctcggacagt ctctgccagc tgtagccgcc gtcgtcggtg
     1981 cggataatca agccgttttc gccgaccgac caacccgtaa aattatcgct gaaatgaagc
     2041 gaaaccaatt tggggcgcgt gcgcacgtta aagggcagcc acgtcgagcc gccgtcgccc
     2101 gtcagccaga ccatcccgtt ggagtcggtc atccagccgt ttttttcatc ggtaaaagcg
     2161 agcgccagaa aatcggcgcg cgtaaagaca ttgattttcg accacgtttc gccggcgtcg
     2221 tcgctgcgca cgacgcagcc gccgtaaccg accgcgatga gtttttcctt gctgagaaaa
     2281 acgacgttgg tcattgccaa ttcggggcac ggagtgttta ctttccacgt aaaaccaccg
     2341 tttttggtcg tcaggataac gccgtttgct ccgaccgccg cgccgtcggt ttgattaaaa
     2401 aacgaaattt tgacgagatg ctcgctcgtg tttgaaatct gttcctgcca cgtcagaccg
     2461 ccgttcgacg tccgcagaat tcgcccgtaa gttccgaccg cccagccgtt ttgctgtccc
     2521 tcgcccgcaa aagtcacgct gtagagatgg tctttgattg gagattttat ttcgcgccag
     2581 tccttgccgc cgttgtcggt cagcaaaatc acgccgcgcg cgccgacggc gacggcgcgc
     2641 agcttgtctt tgacatacac gccgaaaaaa atcgtattga tgggcggaat ctgcgatttc
     2701 cagctaaaac cgccgtccag cgtcgaaaga atcgtgccgt ccgcgccgac ggcaaaaccg
     2761 gtttcgcggt ctttcgcaaa accgatggaa tagagcggat ttccctgtgg cagaggattt
     2821 tgccagaccc agccgagcgc gttttgcttc tgcgcggtaa ttgagccgca cagaacacag
     2881 agaacacaga aagctttaaa aaacccaaag aaagacgcag aaaaaccccg attaaaaaat
     2941 cctgcgagtc cgcgtccaat tgtgtttttc ttcagtttca tttcttctct caatgttcgc
     3001 cgcgcgctct ctctctgcgg ctaaatttcc ttgactccta tttcggcggc ggcttcgtcc
     3061 aaaatcgcct cgtcgacgcg tccgagctcc gccattcgcc gcgccatcga gtcggcgact
     3121 tcctgaactt cgtgtccggc gtgtcctttg acccattccc atttgatttg atgccggctc
     3181 gcttccttgt ccaatttcgc ccaccattcg agattcgttt ttttacgaaa atttcccgtc
     3241 atcgtttcga cgacgtagcg cgaatcggtc aaaaccttta ccttacaagg ctcgcgcagc
     3301 gttttcaagc cgacccacgc cgccgcgatt tccgcctgct ggttcgtcgc gtttcccaag
     3361 tattcgccaa acgcgcgcca gaagccgcga taaccgagca ccgcgaccgc cgccgcgcgc
     3421 gtcgttccct tgccgttgcc gaggcttgag ccgtcgcaga cgattgttac ttctttcata
     3481 aaactacttt aaatttaaac gccgcgacgc aaattttgat tgaatgagtt tgaaaactaa
     3541 tcctaataaa aggctgaata ttattgcaat aaaaatgtcg gcgattaaac cgaaccatag
     3601 atatcgttcg accctaatga ctccgccaga ctcataataa cgaaacggaa atccccacga
     3661 ttgcatacag tcgaaacaga taaatctgtt tgcccctctg tcaacgtata aattaatcgc
     3721 aacaaacaaa atgaatccgg tcacgaatcc gacattgtat gccttatttg taatcaaatt
     3781 tcgcattgct tattcttccg tcgccgccgg attctcgtcc gcctccgcgc tgattgaaat
     3841 cacattatcc acataacctt caaacgtatc gtcatgcgaa atgacgaaaa gctggtcgaa
     3901 atggcggatg ttgccgattt gcgtcgccag attttcgcgc cgcgccgcgt ccatattcgt
     3961 cgtcggctcg tcaaaaaacg ccaaacgcac gtcggaaagc tgtttcagga gcgcgagccg
     4021 gacgcttaaa gccgccgcca tctgctcgcc gcccgacaaa ttggaaaaag gtcggtcgta
     4081 gccgttttcc tccagcacga tgccgtaatc ttccgtccat ttcagcgtcc gctcggcgtt
     4141 tcccgtgatt tcgcggtaca tctgattggc ttccagcgaa acgtgataga cgtaatttcg
     4201 cgcgacgcgc ggcgcggctt ccttcagagt ttcgcggata aaatccgtcg cctcgtggat
     4261 tttttcaagg cgttcctttt cctgaaattc agcctgcatc gtgacgcgga ttactttcag
     4321 tcgccgcaat tcagcgctta attcgctttc gcgtcgcttt gccgcttcaa aagtcgcgcg
     4381 cgtttccgcc agccgttttt ccgcttcgag cagcgcggct ttctcgctca gatgccgttc
     4441 gcggtcataa gtttggctcg cgctttcaaa ttcgccggac gcgttttcga gcccgatttt
     4501 caattgcgcg gcttttccgg tcgcttcggc aaattccgtc tcgcgcgccg cgagcgtttt
     4561 cgccagcgct tcgttcgtca aaaattcgcg atgcgccgcc gccgtccgct cgcgctcgtc
     4621 ggaaaaacgt ttccactcgg cgtccagcgc ctcaaacttc gataactgct cgccgaagcc
     4681 tgatttttcc gtttccagcc gcgccagatt ccgctggatt ttcgataaat tttccgtgaa
     4741 ttcggtttcg cgccgcgcct cgcgctcaaa cgccagcacg cgcgctttcg gattgtccaa
     4801 agatttaagg cggctctcgg tttcggcaat ctgtttttcc agcgacggca gattttcgac
     4861 gtgcgctttc agcgcgtcct gctcggtttt caagcgtatg ccttcttctt taatttccct
     4921 ttcgcgctgg cgataagctt ccagaccgcc gagaaatttt tccgcctcgc gcgccgttcg
     4981 caaggcttca aaaacttctt tctgctcgac ttccaaagtg atgatttgct ttttcgtctc
     5041 ggaaaactgt gttttgataa agccttcgag cgattcgccc ggcttcagat tcagacattt
     5101 ttccgagaga atcgggcaca agccgttgcg aatttcgctc tgaaattttt cgtcgcgcgc
     5161 cagatcggcg cgaaactcgg cgagccggct catcaaatcg ctgtcgcgtt tttgcagaat
     5221 ttcgacctga cgggcgtcgt tggttttcgt ttcggcttcc ttgagcttgg cgttgttttc
     5281 cctaaagctt tcgcgcaggc gcgagatttt ttcttcgagg ctggcaatcg ttttttcaaa
     5341 cgcggcggct ccggcttttt cttttatcaa ttgctcgcgg cgggcttcaa gctcgttttg
     5401 ctcgacggtt tgcggctgga gcgaaacaat cttttctttc gcctcgacga tttttgcgag
     5461 attctcattc agattttttc gctcggcttg cgtcttgata atctcgtttt cgattttcgt
     5521 gagctgattg cgaatgcggt cgcgctcgac acgctgcgcg tccagatttt tcaattcggc
     5581 gagcgccgaa acgtgcgttt gataatcggc ttcgacgagc ttgagctttt ccgacgcggc
     5641 gcgcgcctga ttcgtttccg tttccttttg cccgcgcacg acctcggcgg tcgccagttc
     5701 cgactgcaag cggtcgcggc gcgttttcag ctcattaact tttgtttcga tttcgtcgag
     5761 gctttttacc gccgcgcgtc tttccgccgc ttccgcgttt aatttttcta tgctgccgga
     5821 aagattttcg atttgtccgg cgaccgcctg atgctcgttt tcgaccgttt cgagccgcat
     5881 cagttcgccc tcggcgcggg cgattttctc gcgcgcgtcg gtgattttat tgcgcacgaa
     5941 attgaccgtc tcgcggagct tttccgcgcc ctgacgatat tcttcgacct ttaaaagctt
     6001 gtcgaaagct ttttttcgct cggtcgccgt ttccataaaa atcgccgtaa acgtgccctg
     6061 cggaacgccg atggcgcggc gaaaaagcgt ctccaaatcc gttccggctt cgacgccgag
     6121 atgctgccac aaaaagcgcg agacttcttc ctttttctcg gcgatgcgcg tctttagctg
     6181 cgggtcgaaa acgtaataac cgttggcggt atcgcgataa atgacgtatt cgcgctcgtc
     6241 gagcgagctt tcaaaagtca cgcgcgcgat gcctttcgtc tttgcgccgc gccgcgcgaa
     6301 atcatccttt ttgtaatcga gaaggtcaaa gagcgtccag gcgatggctt cgataatcgt
     6361 cgttttgccc gcgccgtttt cgcccgtaat ggcggtcgtc ccgcgctgaa actcgaaagt
     6421 tgcctgagcg tgagatttga tgttttccag ttcgactcgt gtgatgtgca taaaacagtc
     6481 gtcagcggtc agctatcagc ggtcagtgga agacttctga tttatcgccg gaatttgccg
     6541 caagaatttg attttatccg caaagccgac ggctgacaac cgaccgctgt cggctgacta
     6601 ctgactgctt cttttcagcc tgtccatcaa agccgcgccg attccctttt cttcaaccgt
     6661 ctggcaataa attatttcga cggcgcgacg gtcgcactcg cggaaaaaat taaaaacctc
     6721 gtgcgcgtat tcttcgaccg aacggcagat tttcgtcaaa tcaaaatttt gcgccggttt
     6781 attcaaaccg ataaacgcgc tcgaatctga aatttgaaat cctgaatcct gaatcccgaa
     6841 tcctgaattt accagaataa ccctggcgcg cggcgaataa tgcctgtgtt tcaagcccgg
     6901 acttctgacc gcttcatttt ccttcacccg gtaagtcctg gtttcgggaa ctattttttg
     6961 caaatcttcg agcgacaccg cgcccgcgcg caaaacgagc ggaacatcgc aggtgcaatc
     7021 gacaaccgtc gattcgagcc cgatttcggt catttcccct tgcagaatgc aatctatgcg
     7081 cccgtttaaa tcttcaaaaa ccgcctgcca cgtcgtcggg ctcggcttgc cggaaagatt
     7141 cgccgacggc gcgacgaccg gcgttgcgca ggcttttaaa aattccagag cgagcgggct
     7201 gttcggcatt cgcacgccga cggtctgtag attggcggtc gccgcgagcg gaactttttt
     7261 cgctttcggc aagaccagcg tcagaggcga cgggaaaaac gcttcgataa attcctgcgc
     7321 ggcgggcgtg atttcactgg cgagcaattt aatttgttcg agatttccga cgtgcgcgat
     7381 aagaggatta tccggcgggc gacctttggc gataaaaatt ttctcgaccg cccgctcgtc
     7441 gaaaacatct gcgccgagcc cgtaaaccgt ctcggtcgga aacgccgaca gaccgccgcg
     7501 ccggataaat tcggcggctt tggcgggcga tttggtcaac agcggaaaaa acatatcgta
     7561 atgatggaaa ttcgacttta ttgttctttt taatcttttc tgatttttaa gcgaaagtgc
     7621 aaacagtctt gcattttatc tgaccggggc ggcgaaaaat atttgcgctt cgggaatgtt
     7681 tgctttataa atctacaagc ccattttcaa cccgataaac attttaacta atctaaggag
     7741 aagacgaatg aaaatcaccc taactgtgct tttaatctta ttctttaatc tgattctgca
     7801 aagcggcagc ggctgcggcg gcgagggcgg aaacggcggc ggcagccaat gcccgacgcc
     7861 gttttacccg ccgtatgatg taagagtcag atttgaccct tatcagggcg gaattgtggt
     7921 ctcctggaat gaacgcgggt ataaagacgg ctatcaggta tttcgctgtg tttacaattg
     7981 cgttcaggtc ggacaggacg gaaaatatta tgccaatctc ggaagttttg aagcgataac
     8041 gaacaataga tttgccggca ctttctatgt ggacaaaaat ctttcgcacg gcacgaccta
     8101 tcattacgcg attcagaacc gctatcaatc gtgcggcgtt aactggtcaa ctaattatgc
     8161 gacaattaca gcgccgtaat tcgtatgtca gccgtatttg atttacgtat tgcgtccggc
     8221 tgactttcga aatccgctct gaaaagcaga aaggcaaaag acggaatctt ttgccttttt
     8281 gctttattac tttttacttt tgcctttatc ctagccttca gcctcgcgcg cgatcgccgc
     8341 ttcgccgccg acttttgccg ccacgcgcgc cagaaccaga acgacgatga ccgcgatgat
     8401 cgtcgccaga atcgcgtaag tgtattttga agcgaggctt tcgtctccgt ccataatttg
     8461 tttgatggtc tctttgatgg cgtcgttcca ggccagcgcc gcgaccaaac cgagcgcggc
     8521 ggacgaaagc gcaatcatcg actgcaaaat cattctcgtt gtttctcttg ccatagtttt
     8581 ctcctcttga ttcgaaaaaa tatcctgaaa agcgtgtttg agttattttt tcaaaatgcc
     8641 ggaaaaagtc aatacatttt tcgctttcca ctctcagccg cttcgcgtta gaatttaatc
     8701 aatgacgctg gaagaaaaac tgaaaaattt gcccgtcgcc gcgggcgtct acctgcacaa
     8761 aaacgcggac gggaaaatca tctacgtcgg caaggcgaaa aatctgcgca accgcgtccg
     8821 gcagtatttt caatcctcgc gcaaccagga ccgcaaaacg cgcgagcttg tcaggcgcat
     8881 cacggatttc gagtttatcg tcgtcgacag cgaagtcgaa gcgctcgttc tggaatcgaa
     8941 tctcatcaaa aaacacaagc cgcgctttaa cgttttatta aaagacgaca agcagtatcc
     9001 gcatctgaaa atgacgaacg agccttttcc gaaagtcgtc attaccagaa aaatcctgcg
     9061 cgacggcgcg agttattacg gaccttttct gcccgccaat ctggcgcgaa agactttgga
     9121 tttaatcaac cgcacgtttc agctgcgcac ctgcgacatc gaaatcgacg ggaagctgcc
     9181 gcgtccgtgt ctggaatatc acttgaagcg ctgtctcgcg ccgtgcgtca aggagctttg
     9241 caaacccgac gaatacgcgc aggcggcaag cgacgttaaa ttattgctcg aaggcaaaaa
     9301 caaggaatta gccgacgcgc ttcagcagag aatgtggcat ttcgccgaga cggaaaacta
     9361 cgagctggcg gcgaaatatc gcgatttgca caaaaccgtg ctggcgctcg gcgagcagca
     9421 gaaaatggcg acgaccgccg agcgcgacgt cgatattttc ggcttttacc gcgagggcac
     9481 gcggctcgcg ctccagcttt tcacgatgcg cgaaggcaag atagtcggtc ggcgcgagtt
     9541 tttctgggaa gatttggacg ccgataattt cagcatctcc gattttctga gcgaggttct
     9601 gtcgcagtat tattcgaccg attacgtgcc gctcgaaatc aacgtgcccg ccgatttcga
     9661 agaccgcgag attttagaaa aggctctgac ggaaaggcgc gggcgaaagg tgcggattct
     9721 cgacccgaag cgcgggcaga agcgcgagat gatcgcgctg gtcgaaaaca acgcgaaaat
     9781 cgctttcgaa cagcgtttcc gcgtgctcca gcccgccgcg cagaaactgc tggaagattt
     9841 gatggagatt ctggagctgc cgcatttccc ggagcgcatc gagtctttcg acatctcgaa
     9901 catttccggc gcggaaaacg tcgccggcat cgtcgtctac gaaaacggca agccgaaccg
     9961 cgccgagttt cgccgcctga taatcaaatc ggtcgaaggc gcaaacgatt tcgcctcgat
    10021 gaacgaagcc gtctttcgcc gctacaagcg tcttttgaat gaagacaagc ccctgccgca
    10081 gctggttttc atcgacggcg gcaaagggca gttaacggcg gcggcggcgg cgatgcaggc
    10141 gctggatttg gaaggcgtca taacgctggt cggtctggtc aagccgccga agcggcataa
    10201 tgaaatctct catcttttgc gcttcggctc cgaggataga ccgatttatt tcgagatggg
    10261 cgttccggct tttcgcctcg tccagcaaat ccgcgacgaa acgcaccgca ccgccatcca
    10321 gtatcatcgc aagcgccgcg agatgcgcga tttcacgtcg gagctgaccg ccattcccgg
    10381 catcggcgac aagcgcaagc tgaagcttct gagaaacttc ggctcgctcg aaaaagtcgc
    10441 ccaggcaagc gccgaacagc tcgcgccctt cgtcggagcc aaagccgcgc aggaaatttt
    10501 cgagcatttc gagcggcaaa gaaagcttgc ggaaaactaa cttccaatcg tttccgccgc
    10561 gccgccgccc gaaatcgcgc gaaaaaactt cttgaaattc attttattcg gtgctaatct
    10621 cgcatcacaa aaccgtcgac gcgcattttc aaagcagccg aacgcttcaa aaaacgcgcg
    10681 ccgccggttg ccgacaaagt ccagcccacg gtctttgaca tcccgacaaa acacccttcc
    10741 agccattcga cgaccgtttc agctcgttcg acgaacattt caacttgttc gacgaacatt
    10801 tccatacgtg cagcggcggc gtcaaatcat tcaacgcccg cttcaggacg cgcaacggac
    10861 acttcaactc gcgcaacggg catttcaagt cattcaacga acagttaaag tcgtgcagta
    10921 aagacttcga ctcgtgcaat gaacacttca agtcgtgtaa tgaacacttc aagtcgtgca
    10981 atgaacactt caagtcgtgc aatgaacact tcaactcgtg caatgaagac tttaacttgc
    11041 gtaacaaaga cttcgactcg tgtaacgggc atttcaagcc gttcaatcat cgtttcgact
    11101 cgattaagcg cgctttccaa agcccgcgga tgcattaaga cataccgcat cgcggacaat
    11161 aatcaaccgt tctttaatat cgaccggcaa cgcttacaaa ataaaattcg atgtgaaaag
    11221 gagataaatt gaaatgaaat cagcccagca ggcaaaactt aatatgtatg acgccgttaa
    11281 aacc
//
LOCUS       DASWNE010000832         2608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_593175, whole genome shotgun sequence.
ACCESSION   DASWNE010000832 DASWNE010000000
VERSION     DASWNE010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593175"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VGB68_16325"
     CDS             complement(<1..323)
                     /locus_tag="VGB68_16325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050860.1"
                     /translation="MNRKLIVCFSFLLAFVFAQNAFAQTDLSDYEQARREGWKAFDKQ
                     NWQKFDFTKRKITRAQLEKLDVSAAFSQLELLRGVVFGKRGRVFKERSIQDYLEKQAW
                     YKANP"
     gene            complement(420..839)
                     /locus_tag="VGB68_16330"
     CDS             complement(420..839)
                     /locus_tag="VGB68_16330"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY0050861.1"
                     /translation="MSDYKATIQYAGNEFFIGTSPSGHAQVIDGKGERKSAASPLELL
                     LIAVGACTAVDVVSILEKKRQTVTDYKIIVEGERREEYPRAFTKIHVHHIVYGRNVSE
                     QAVAGAVRLSDEKYCSVAATVRPTAQIQTSFEIIEVE"
     gene            1029..>2608
                     /locus_tag="VGB68_16335"
     CDS             1029..>2608
                     /locus_tag="VGB68_16335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050862.1"
                     /translation="MNRHRNVFSALLAIGLIFFASHAQQVSAQRTQQVNEREVRSLLR
                     QLTVKLDDFQYNLDSELRQNSASQTDEEDLRNSLDGLQTEINNFQNKFTRRRDSQSDV
                     SQILSSARTVEENLSRLRLSQKMRREWIDTQKLLDQLANNYGVRPDSNDDDNSQLPDD
                     QTPSTQYPNPTPNTNFSYGLTGTYQLDASRSENAADVAERAVGGTNTQNREEARRDLQ
                     EKLEAPERLAIDVKGNQVTLASSLATQVTVTADGRDRTETLSDGRTLRVRTTLRGQEL
                     TVSSVGGNNDYTVTFASIENGKSLKVTRRVTTDYLNQTVFAESVYTKTDSIARYDIYN
                     TNPTPNTNYPPTTTNGRTGEFIVPNGTIITGNLENDITTKVSQNNDRFRMIVSAPNQF
                     RGAVIEGYISGINRSGKVSGRSQLTLNFERIKMPTGETYDFAGFLTSVTDQNGKTVKI
                     DTEGSVKGDSQTKETVKRGGIGAGLGAIIGAIAGGGKGAAIGAIIGGGAGAGSVYAQG
                     REDLELKAGSSITVQASSP"
BASE COUNT          652 a          704 c          670 g          582 t
ORIGIN      
        1 ttcggattcg ctttatacca ggcttgtttt tccagataat cctgaatcga gcgttccttg
       61 aaaacgcgcc cgcgtttgcc gaaaacgact ccgcgcagca gctcgagctg gctgaaggcg
      121 gcggaaacgt cgagcttttc aagctgggct ctggtgattt tccttttcgt aaaatcgaat
      181 ttttgccagt tctgcttgtc aaacgctttc cagccttctc ttctcgcctg ttcgtaatcc
      241 gataaatcgg tctgcgcgaa agcgttttga gcaaaaacga acgccagcag gaaactaaaa
      301 caaacaatta attttctgtt catatttctt ttctttgtca tttttgcgtg gaaaatttaa
      361 ttttaatcaa attacttttc ataatttccg tatttaattt ataaagactt cgccgtttac
      421 tattcgactt cgataatctc aaaactcgtc tgtatctgcg ccgtcgggcg gacggtcgcc
      481 gcgacgctgc aatatttttc gtcggaaagc cgaaccgcgc cggcgacggc ttgctcggaa
      541 acgttgcgac catacacgat atggtgaacg tgaatttttg taaaggcgcg cgggtattct
      601 tcgcgccgct cgccctcgac gatgattttg taatcggtca ccgtctgccg ttttttctcc
      661 agaatcgaaa cgacgtcgac cgccgtgcag gcgccgacgg cgattagaag cagctccagc
      721 ggcgaagcgg cggattttct ttcgcctttg ccgtcaatga cttgcgcgtg accgctcggg
      781 ctggttccga taaaaaactc gtttccggcg tattgtattg tcgctttgta atcagacatt
      841 ttccctttgc ttgaacgaaa gaataaaata attttgcgcg attctaacac aaaaaccgtc
      901 tattttaata aaagattttt cgacaactat tcggcagagc aaaacaacta atttactggc
      961 acaaaaaatg ctgtcagagt gtgcgtcata actttatacg gggacgtttt tcggaagaag
     1021 gagaaaatat gaacaggcat cgaaacgttt tttcagccct tttggcaatc ggcttaatat
     1081 tttttgcatc gcacgcgcag caagtcagcg cgcagcgcac gcagcaggtc aacgagcgcg
     1141 aggtgcgcag cctgctgcgc cagctgaccg tcaagctcga cgattttcaa tataatctgg
     1201 attctgagct gcgtcagaat tccgccagcc agacggacga agaagatttg agaaacagcc
     1261 tcgacgggct gcaaacggaa atcaataatt ttcaaaataa attcacgcgc cgccgcgaca
     1321 gccagtcgga cgtttcgcag attctctcgt cagcccggac ggtcgaagaa aatttgtctc
     1381 gcctgcgctt aagtcaaaag atgcgccgcg aatggattga cacgcagaag cttctcgacc
     1441 agctggcgaa taattacggc gtgcgcccgg attcaaacga cgacgataac tcgcagctgc
     1501 cggacgatca gacgccgtcc acgcaatacc cgaatccgac gccgaacacc aatttcagct
     1561 acggcttgac cggaacttac cagctggacg cgtcgcgcag cgaaaatgcc gccgatgtcg
     1621 ccgagcgcgc ggtcggcggg acaaatacgc agaaccgcga agaagcgcgg cgagatttgc
     1681 aggaaaagct cgaagcgccg gaacgcctcg ccatcgacgt caaaggaaat caggtgacgc
     1741 tggcttcttc gctcgccacg caggtgacgg tcacggctga cggtcgcgac cggacggaaa
     1801 ctctgtcgga cggcagaacc ctgcgcgtgc gcacgaccct gcgcggtcag gaattgaccg
     1861 tctccagcgt cggcggcaac aacgattaca ccgtgacttt cgcttctatc gaaaacggca
     1921 aaagcctgaa ggtgacgcgc cgcgtgacga ccgattatct gaatcagacc gttttcgccg
     1981 aaagcgttta tacgaaaacc gattcgatcg cgcgatacga catttacaac accaacccga
     2041 cgccgaacac gaattatccg ccgactacga ccaacggtcg cacgggcgaa tttatcgtgc
     2101 cgaacgggac gattatcacc ggcaatctcg aaaacgacat tacgaccaaa gtctcgcaaa
     2161 acaacgaccg ttttcggatg attgtttccg cgccgaatca atttcgcggc gcggtcatcg
     2221 aaggttatat ttcgggaatc aatcgttcgg gcaaagtttc gggacgctcg cagctgacct
     2281 taaactttga aagaatcaaa atgccgacgg gcgagactta cgatttcgcc ggttttctga
     2341 ccagcgtcac cgaccagaac ggcaaaaccg ttaaaatcga caccgaaggc tcggtcaaag
     2401 gcgacagcca gaccaaagaa accgtcaagc gcggcggcat cggcgccggc ttgggcgcga
     2461 ttatcggcgc gatcgcgggc ggcggaaaag gcgcggcgat cggcgcgatt atcggcggcg
     2521 gcgcgggcgc cggctcggtt tacgcgcaag gcagagaaga cctcgaactg aaagccggtt
     2581 cgtcgattac cgtgcaggct tcttcgcc
//
LOCUS       DASWNE010000833         2272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_520943, whole genome shotgun sequence.
ACCESSION   DASWNE010000833 DASWNE010000000
VERSION     DASWNE010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520943"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1729
                     /gene="thrA"
                     /locus_tag="VGB68_16340"
     CDS             <1..1729
                     /gene="thrA"
                     /locus_tag="VGB68_16340"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF006959.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HEY0050863.1"
                     /translation="VTYREAMELSHFGAKVIYPPTIQPVMAKGIPVRVKNTFAPDDFG
                     TLIEAEILEEKEIIRGISSIDRISVLNLEGSGMVGIPGFSRRLFGALARAQINVILIT
                     QSSSEHSICVAIEESCGRLAKQIVDREFEYEIAGGKIEPLRVENGFSILALVGDNMKN
                     HPGISGKMFAALGAQGVNIHAIAQGSSERNISTIISSNDVRKAVNTLHEEFFSDGKKQ
                     INLFVVGVGTVGSRLLEQLKQQQQYLSEKMRLNVRVVGLANSRRMIFDEEGIDLTGWR
                     EKLNEESSEARRAKPGEQSSDFANLIIEKNLRNSIFVDITASAAIVETYAKLLAKSVS
                     IIACNKIACSSEFAEYQNLKDLAREFNTVFLFETNVGAGLPVINTLNDLMRSGDKINR
                     IEAVLSGTLNFVFNNYDGARSFSTVVRQAQAEGYTEPDPRLDLSGTDVARKILILARE
                     AGFKLEMADVENKSFLPESCLQGTVEDFYIEMEKHESYFRRLLESAKARDLSLKYIAS
                     FDEGKASVGLQSIDRAHDFANLSGKDNAVLFYTNRYVGLPLVIKGAGAGADVTAAGVF
                     ADIIRAARI"
     gene            1828..2193
                     /locus_tag="VGB68_16345"
     CDS             1828..2193
                     /locus_tag="VGB68_16345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050864.1"
                     /translation="MLLINIAVKSFSDSIRIHPGERLAQKNKETFIFNPEPHYLYKAK
                     RELNQYFFCEAIGKRRSPKEKRHLPIGKQDLKVRKHHFLIRKLEILTSKSRLLTGKRQ
                     LLTGKPRLIVSKRLLIVSK"
BASE COUNT          618 a          531 c          582 g          541 t
ORIGIN      
        1 cgtcacctac cgcgaagcaa tggagctttc gcatttcggc gcgaaggtga tttacccgcc
       61 gacgattcag cccgtgatgg cgaaaggcat tccggttcgg gtgaaaaaca cgtttgcgcc
      121 ggacgatttc ggcactttga ttgaagcgga gattttggag gaaaaggaaa tcattcgcgg
      181 catttcgagc atcgaccgaa tctcggtttt gaatctcgaa ggcagcggaa tggtcggcat
      241 tcccggcttt tccagacggc ttttcggcgc gctcgcgcgc gctcaaatca acgttattct
      301 gattacgcag agttcgtccg agcattcaat ctgcgtcgcc atcgaggaaa gctgcggtcg
      361 gctggcgaaa caaatcgtcg accgcgaatt cgaatacgag attgcgggcg gaaaaatcga
      421 gcctttgcgc gtggaaaacg gcttttcgat tctcgccctg gtcggcgaca atatgaaaaa
      481 tcatccgggc atcagcggaa aaatgtttgc cgcgctcggc gcgcagggcg tcaacattca
      541 cgccatcgcg cagggctctt cggaaagaaa tatctccacg attatcagct cgaacgacgt
      601 gcgcaaagcc gtcaacacgc tgcacgagga atttttctcg gacggcaaaa aacaaataaa
      661 tcttttcgtc gtcggcgtcg gcacggtcgg cagccgcctt ctcgaacagc tcaaacagca
      721 gcagcaatat ctgtcggaaa agatgcgctt aaatgtgcgc gtcgtcggct tggcaaacag
      781 ccgccgaatg atttttgacg aagagggaat cgatttgacc ggttggcggg aaaagctgaa
      841 cgaagaatct tccgaagccc gaagagcgaa gcccggagag caaagctcgg atttcgctaa
      901 tttaatcatc gaaaaaaatc tgcgaaactc gatttttgtc gatatcacgg cgagcgcggc
      961 aatcgtcgag acatacgcga agctgctggc aaaaagcgtt tcgattatcg cctgcaataa
     1021 gattgcctgt tcttcggaat ttgccgaata tcagaacctg aaagatttag cgcgcgaatt
     1081 caacacggtt tttctgtttg aaaccaacgt cggcgcggga cttcccgtca tcaatacgct
     1141 caacgatttg atgcgcagcg gcgataaaat caaccgcatc gaagcggttt tgtcgggaac
     1201 tctgaatttt gtttttaata actacgacgg cgcgcgctcg ttttccacgg tcgttcgaca
     1261 ggcgcaggcg gaaggctaca ccgagcccga cccgcgactc gatttaagcg ggacggacgt
     1321 ggcgcgaaag attttaattc tggcgcgcga agccggtttt aaactggaaa tggctgatgt
     1381 cgaaaacaaa agctttttgc ccgaatcgtg tttgcaggga acggtcgaag atttttatat
     1441 cgaaatggaa aaacacgaaa gctattttcg ccggcttctg gaaagcgcga aagcgcgaga
     1501 tttatctttg aaatacatcg cttcattcga cgaaggcaaa gcctcggtcg gtttgcagag
     1561 catcgacagg gctcacgatt tcgccaatct ttcgggcaag gacaacgccg ttttgtttta
     1621 cacgaatcgt tatgtcgggc tgccgctcgt cataaaaggc gcgggcgcgg gcgcggacgt
     1681 gacggcggca ggcgttttcg ccgatatcat tcgcgcggcg agaatttaaa aatcgaccgc
     1741 gaaataataa aatcatttgc cggcgaggcg attcgagaaa ttcagtttcg gataaaagct
     1801 cgatcggttt tctttgagcc ggcgcggttg cttttaataa atatcgccgt gaaaagtttt
     1861 tccgattcaa tccgcattca cccgggcgaa aggctcgcgc agaagaacaa agaaacattt
     1921 atttttaatc ctgaaccgca ctatttatat aaagccaaac gcgaattaaa tcaatatttt
     1981 ttttgcgagg caatcggaaa gcgccgctcg ccgaaagaaa aacgtcactt gccgatcgga
     2041 aaacaggatt tgaaagttag aaaacatcat tttcttatta gaaaacttga aattctaact
     2101 agtaaaagcc gcttactaac tggtaaacgt caattactaa ctggtaaacc gcgtttgata
     2161 gttagtaagc ggcttttgat agttagtaaa tgagacttac taatcagaaa acggcgttta
     2221 ctagttagta aatcggaaat actaactagt aaacgccgtt ttctttctta aa
//
LOCUS       DASWNE010000834         6136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_663910, whole genome shotgun sequence.
ACCESSION   DASWNE010000834 DASWNE010000000
VERSION     DASWNE010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663910"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..1412
                     /gene="wecB"
                     /locus_tag="VGB68_16350"
     CDS             186..1412
                     /gene="wecB"
                     /locus_tag="VGB68_16350"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEY0050865.1"
                     /translation="MEKIDNKSAEASSAAVGEINDSRRKVLVLFGTRPEAIKLAPVIH
                     ELRAKNFRTVVVSSSQHKDLLKPFLKLLAVEIDYDLKVMSRNQTPTEVCSRVLSKLDK
                     ILDAEKPDLILVQGDTTTTLAGALAGFNRKIKVGHIEAGLRSGNLYSPFPEEMNRRLT
                     SQITSFHFAATEKNKRNLLAEGVSNEKIFVAGNTVVDSLQFILKNLVPSEKISALIEQ
                     TKGLKRILLTTHRRESFGETMSGNLQVMADFVEKRKNVCLIFPVHPNPNVREATEEIL
                     ADRERIFLLEPLDYMDFVALMKKSWLIVSDSGGVQEEAPSLGKPLLILRENTERPEAV
                     ESKIARLVGGNPKVLRKMLEENYAIETWIKSVREIKNPFGDGRSAKKIVEILEKEFRA
                     KTQRTPGTMASGLKPF"
     gene            1522..3228
                     /locus_tag="VGB68_16355"
     CDS             1522..3228
                     /locus_tag="VGB68_16355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0050866.1"
                     /translation="MNQPRENKKHLLTVLVEDYFHVGAFENLIQQRNWSRFEPRYEQN
                     TLKTLDLLDEFDTKATFFVLGWIAEQNPKLIREIIARGHEVASRGFYHRSLRQLTPEE
                     FREDLRKSNRALEDAGGQKIIGYRAAEKLSYEKDAWIFDILAEEGFVYDASFLPSRKQ
                     EKPKRFAHQVHTGGKAIWEFPYSTRDFGLGLLPISGGNYFRQIPYTLMRKSVRQWTEN
                     HDEPFVFYFHVWELDPEQPRISAASSYNRVRHYRKLDKMEWILKENLQLYDFVDAAEF
                     LGLKEEIKVESRESRVESPIVELRTQNSALGTPISIVIPCYNEEPTLPYLANTLKSLE
                     EGLSRNYKPEFIFVDDCSKDATFERLQEIFGAKENVRIVRHEINQGVAAGIMTGMCEA
                     ATEIVCSMDCDCTYDPHELEKMLPLLSENVDMVTASPYHAQGGVRNVPEWRLFLSKGA
                     SWLYRRVLRSKLDTYTSCFRVYRRSSVIDLNLRETGFLGVAEMLGKLDLGGGRIVEYP
                     AVLEVRLFGFSKMKTARTIFGHLKLLSHLAKLRFFGNKDAIKTSLPKEKLKPQIYTDK
                     HK"
     gene            3336..4562
                     /locus_tag="VGB68_16360"
     CDS             3336..4562
                     /locus_tag="VGB68_16360"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY0050867.1"
                     /translation="MTPTLQPEIIKLPSDQDASGRTFGAEEIAALTEVLQSGTLITTK
                     GKFGKLLEESFAKRLGAKYAYACNSGSAAVHTAIAAVNPNPGDEIVTTSITDMGALTP
                     IIFQGAIPVFADVDPKTLNVTAETIEKVLSEKTKAIIVTHLFGNPCEMKAIMELADSR
                     NIPVIEDTAQSFLAEYDGKYVGTIGKIGAFSFQQGKHMTTGEGGIVVTNDDEIARRMY
                     LFINKAWGYGDANPDHYFASLNYRMTELQAALAYEQLKKLDACVEQRQRMARLLDEKI
                     AGIDGIESYKPAESSTMTYWKYCLRVDDRLIEDGAVGLAKALKAYDVASAPRYVVKPA
                     YKCRVFTEQNTFGDSHYPFNLARPEAVDYNDDNFQGTLKGLHDVLVLPFNERYEEKHV
                     EFLASSIREAVENTRK"
     gene            4680..5684
                     /locus_tag="VGB68_16365"
     CDS             4680..5684
                     /locus_tag="VGB68_16365"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY0050868.1"
                     /translation="MEKLKFALIGTGGIAQTYAQAFQTSRCCRLVAVADVRKESADAF
                     AEPFAAKSYADYKTLAENESVDAVIVSTPPDSHPEIAMFFMNRGVHVLCEKPLCLRAA
                     EAREMLDCAEKTGVKFTMASKFRYVEDAIKAKSMVASGILGDVVQFENAFTAKVDMSR
                     RWNSDKAASGGGVLIDNGTHSVDIIRYFLGAIEEVLAVETGSTQNLVVDENVKMFVKT
                     KNGIAASIDLTWGINKELPYFISIYGTSGTLHIGWRESKYKLNSNPDWIQFGTGYDKV
                     QAFRSKIENFARAIGGTEDLLIKPEDALASVEVIEAAYKSLNQNLWQKVIEKSGAAAR
                     "
     gene            5738..6010
                     /locus_tag="VGB68_16370"
     CDS             5738..6010
                     /locus_tag="VGB68_16370"
                     /inference="COORDINATES: protein motif:HMM:NF014821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HEY0050869.1"
                     /translation="MENKTYALTGADGKPYQSASKGELGGHRGDKIYGTLDCAGAKMW
                     IAKGHYVKQRVFFADEETAIAAGFRPCARCLPEKYRQWKAARNLQK"
BASE COUNT         1636 a         1476 c         1630 g         1394 t
ORIGIN      
        1 accggttttc acgaagttaa cttgcatttt tcaaatttcg cttggagaaa tccttataaa
       61 atgtcagaat ctcgccggag cagccgcttc ttttccaacc cgaaagattt taaaaggaat
      121 taaaggcttt ttataaattt agtagaactg ggttttacgc gttaattcgt gtgtccctaa
      181 attaaatgga aaaaatagac aataaaagcg cagaagcatc atcagccgcg gtcggcgaaa
      241 tcaacgattc gcggcgtaaa gttctcgtct tgttcggcac gcgtcccgaa gcgataaagc
      301 tggcgcccgt cattcacgag ctgcgggcga aaaattttcg caccgtcgtc gtttcgtcga
      361 gccagcataa agatttgctg aagccttttt tgaagctgct cgccgtcgaa atcgactacg
      421 atttgaaagt aatgagccgg aatcagacgc cgaccgaggt ttgctcgcgc gtgctttcaa
      481 agctggacaa gattctggat gcggaaaagc cggatttgat tctggtacag ggcgacacga
      541 cgacgactct ggcgggcgcg ctcgccggat ttaaccgcaa aatcaaggtc ggacacatcg
      601 aggcgggctt gcgctcggga aatctttaca gcccgtttcc cgaagagatg aatcgccgcc
      661 tgacgtcgca gataacgagc tttcattttg ccgcgaccga gaaaaacaag cgcaatctgc
      721 tggcggaagg cgtttcgaac gagaaaattt tcgtcgccgg aaatacggtc gtcgactcct
      781 tgcaatttat tctgaaaaat ctcgtgccga gcgaaaaaat cagcgcgctg attgaacaaa
      841 ccaagggctt gaaacgcatt ttgctgacga cgcaccgccg cgaaagcttc ggcgaaacga
      901 tgagcggaaa cttgcaggtg atggctgatt ttgtcgaaaa aagaaaaaat gtctgcctga
      961 tttttcccgt tcatccgaat ccgaacgtgc gcgaggcgac cgaagaaatt ttagccgatc
     1021 gagaaagaat ttttctgctc gagccgctcg attatatgga ttttgtcgcg ctgatgaaaa
     1081 aatcgtggct gattgtctcc gattccggcg gcgtgcagga agaagcgccg agcctcggaa
     1141 aaccgcttct tattcttcgt gaaaacaccg agcgtcccga agccgtcgaa tcgaaaatcg
     1201 cgcggctggt cggcggcaat ccgaaggttt tgcggaaaat gctcgaagaa aactacgcga
     1261 ttgaaacgtg gataaagagc gtgcgcgaaa tcaaaaaccc gtttggcgac ggcaggagcg
     1321 cgaaaaaaat cgtcgaaatt ctggaaaaag aatttcgcgc gaaaacgcaa agaacgccgg
     1381 gaacgatggc gagcggttta aagccgtttt gaaatttcga acctgaaatt cagtctcagg
     1441 gcttcgttgt aaaatctgaa attgaaatct gaaaccagag tctcggattt taatttcaaa
     1501 tttggaaatc cggaatttgt gatgaatcaa cctcgcgaaa ataaaaagca tcttttgacc
     1561 gttttggtcg aagactattt tcacgtcggc gcgtttgaga atctcatcca acagcgaaac
     1621 tggtcgcgtt ttgagccgcg ttacgaacag aacactttaa aaactttgga tttactggac
     1681 gaattcgaca cgaaagcgac gtttttcgtg ctcggctgga ttgcggaaca gaatccgaag
     1741 ctcattcgcg aaatcatcgc gcgcggtcac gaagtagcct cgcgcggttt ttatcatcgc
     1801 tctttgcgtc aattaacgcc cgaagaattt cgcgaggatt tgcgcaaatc caatcgggcg
     1861 ctggaagacg ccggcgggca gaaaattatc ggctatcgcg cggcggaaaa gctttcctac
     1921 gagaaagacg cgtggatttt cgacattctg gcggaagaag gctttgttta cgacgcttcg
     1981 tttctgccgt cgcggaaaca ggaaaaaccg aagcgtttcg cgcatcaggt gcacacgggc
     2041 ggcaaggcaa tctgggaatt tccctattcg acgcgcgatt tcggcttggg actgctgccg
     2101 atttccggcg gaaattattt tcgccagatt ccctacacgc tgatgcgcaa atcagtccgc
     2161 caatggacgg aaaatcacga cgagccgttt gttttttatt ttcacgtctg ggagctcgac
     2221 ccggaacagc cgcgcatctc ggcggcttcg agctataatc gcgttcgcca ttaccgcaag
     2281 ctcgacaaaa tggaatggat tttaaaggaa aatctacagc tttacgattt tgtcgatgcc
     2341 gccgaatttc tcggactcaa ggaagaaatc aaggtcgaga gccgcgagtc gagagtcgag
     2401 agtccgattg ttgagctcag aactcagaac tcggcactcg gcaccccgat ttccatcgtc
     2461 attccgtgct acaacgaaga accgaccttg ccttatctgg caaatactct gaaaagtctg
     2521 gaagaaggtt taagccgaaa ttacaagccg gaatttattt tcgtcgacga ttgcagcaag
     2581 gacgcgactt ttgagcgatt acaggaaatt ttcggcgcga aggaaaacgt gcggattgtc
     2641 cggcacgaaa tcaatcaggg cgtcgcggcg ggaattatga ccggaatgtg cgaggcggcg
     2701 acggaaatcg tctgctcgat ggattgcgac tgcacttacg acccgcacga gctggaaaaa
     2761 atgctgccgc ttttgagcga aaacgtcgat atggtgacgg cttcgccgta tcacgcgcag
     2821 ggcggcgtcc gcaacgttcc ggaatggcgt ctgtttttgt cgaaaggcgc gtcgtggctt
     2881 taccggcgcg ttttgcgctc gaagctcgac acttatacaa gctgttttcg cgtctatcgc
     2941 cgcagctcgg tcatcgattt aaatctgaga gaaaccgggt ttttgggcgt cgcggaaatg
     3001 ctcggcaagc tggatttggg cggcgggcgc attgtcgaat atccggcggt gctggaagtc
     3061 agattgttcg gattttccaa gatgaaaacg gcgcggacga ttttcgggca tttaaaactg
     3121 ctttcgcatc tggcaaaatt gcgttttttt ggaaataagg acgctattaa aacttcttta
     3181 ccaaaagaaa agctgaaacc acaaatttat acggataaac acaaatgaac taaccgagga
     3241 tgaagggata aaggagataa agacaaaaca ttaaataatc atttcttttt atccctgtta
     3301 atctccttta tcctcgattg atttccctaa aaaatatgac accgacatta caaccagaaa
     3361 ttatcaaact gccgtccgac caggacgctt cgggcagaac cttcggcgcg gaagaaatcg
     3421 ccgccttgac ggaagttctg caatcgggaa ctttaatcac gaccaaaggc aaattcggca
     3481 agcttttgga agaaagcttt gccaagcgtc tgggcgcgaa atacgcctac gcgtgcaatt
     3541 ccggctcggc ggctgtgcat acggcgattg ccgctgtaaa tccgaatccg ggcgacgaaa
     3601 tcgtcacgac ttcgataacc gatatgggcg cgctgacgcc gattatcttt cagggcgcga
     3661 ttccggtttt tgcggacgtc gacccgaaaa ctctgaacgt cacggcggag actatcgaaa
     3721 aagttttgag cgagaaaaca aaagcgatta tcgtcacgca cctgttcgga aatccgtgcg
     3781 agatgaaggc gattatggag cttgccgatt cgcgaaacat tccggtaatc gaggacacgg
     3841 cgcaaagctt cctcgccgaa tatgacggaa aatacgtcgg caccatcgga aaaatcggcg
     3901 cgttttcctt ccagcaaggc aaacatatga cgacgggcga aggcggaatc gtcgtcacga
     3961 acgacgacga aatcgcgcgc cggatgtatc ttttcatcaa caaagcgtgg ggctacggcg
     4021 atgcgaatcc cgaccattat ttcgcttcct tgaactatcg aatgaccgaa ctgcaggcgg
     4081 cgctggctta cgagcagttg aaaaagctcg acgcctgcgt cgagcagcgg cagagaatgg
     4141 ctcggctgct ggacgaaaaa atcgcgggca tcgacggcat cgaaagctac aaaccggcgg
     4201 aaagctcgac gatgacttac tggaaatact gcctgcgcgt cgacgaccgc ttaatcgaag
     4261 acggcgcggt cggtctggca aaagccctga aggcttacga tgtcgcttcc gcgccgcgct
     4321 acgtcgtcaa accggcttat aaatgccgcg ttttcaccga gcagaacacc ttcggcgaca
     4381 gccattaccc gtttaatctg gcgcgtcccg aagcggtcga ttacaacgac gataattttc
     4441 agggaacgct caaaggcttg cacgacgttc tcgttctgcc ttttaacgag cgctacgagg
     4501 aaaaacacgt cgaatttctg gcttcatcaa tccgcgaagc ggtcgaaaat acgaggaaat
     4561 aatttagcgg gagaacggta agggattaga tgaataagtg attaagtgtt tatttgacga
     4621 gaaccgcatt ttgcataatc gcctaaacgc ctaatccctt cttccctttg aataaaatta
     4681 tggaaaaact caaatttgca ttaatcggaa cgggcggcat cgctcaaact tacgcgcagg
     4741 cttttcagac cagccggtgc tgccggctcg tggcggtggc ggacgtgcga aaggaatcgg
     4801 cggacgcgtt tgccgagcct ttcgccgcga aaagctacgc ggattacaaa acgctcgcgg
     4861 aaaatgagag cgtcgacgcc gttatcgttt ccacgccgcc ggattcgcat ccggaaatcg
     4921 cgatgttttt tatgaatcga ggcgttcacg tgctttgcga aaaaccgctt tgcctgcgcg
     4981 ccgcggaagc gcgggaaatg ctcgattgcg ccgaaaaaac cggcgtaaaa ttcacgatgg
     5041 cttcaaaatt tcgctacgtc gaagacgcca tcaaagcgaa atcgatggtc gcctccggca
     5101 ttttgggcga tgtcgtgcag tttgaaaacg ctttcacggc gaaagtcgat atgtcgcggc
     5161 gctggaattc ggacaaagcg gcgagcggcg gcggcgtttt gattgataac ggcacgcatt
     5221 cggtcgatat tatccgttat tttctcggcg caatcgaaga agtgctggcg gtcgagaccg
     5281 gctcgacgca gaatcttgtc gtagatgaaa acgtcaagat gtttgtaaaa accaaaaacg
     5341 gcatcgccgc cagcattgat ttgacgtggg gaattaataa agaattgcct tattttataa
     5401 gcatctacgg cacgagcgga actttgcaca tcggctggcg cgaatcgaaa tacaagctca
     5461 attcaaatcc cgattggatt cagttcggca cgggctacga taaagtgcag gcgtttcgct
     5521 cgaaaatcga gaatttcgcc cgcgccatcg gcggcacgga agatttgctg attaaaccgg
     5581 aagacgcgct cgcctcggtc gaggtcatcg aagcggcgta taaatcgctg aaccagaatc
     5641 tgtggcagaa agtgattgaa aaatccggcg cggcggcgcg ctgatacgtt aacgaggcga
     5701 aactcggctg gcgtcgcgcg cgaaaagttc gggatttatg gaaaataaaa cctacgctct
     5761 taccggcgca gacggcaagc cttatcaaag cgcatcgaaa ggcgagcttg gcgggcatcg
     5821 cggagataaa atctacggaa cgctcgactg cgcgggcgcg aagatgtgga ttgccaaagg
     5881 tcattacgtc aaacagcgcg tctttttcgc cgacgaagaa acggcgattg cggctggctt
     5941 tcgtccctgc gcgcgctgtc tgccggaaaa atacaggcaa tggaaagccg caaggaattt
     6001 acagaaataa gcaggcttcg cggcttgccg aaccaggggt taagtacaat ctgcaaatat
     6061 ctatttatcc ctgacaatcg ctgttattcc ggcaattttc cttgttttct cttttgtaaa
     6121 attaaatggt aaagat
//
LOCUS       DASWNE010000835         3283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_132156, whole genome shotgun sequence.
ACCESSION   DASWNE010000835 DASWNE010000000
VERSION     DASWNE010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132156"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VGB68_16375"
     CDS             <1..627
                     /locus_tag="VGB68_16375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050870.1"
                     /translation="ASFSMRFLLFLCFILWLSTAVGGVFYLARYENTPAGKNFSYPPA
                     FPPESHIKRDVQRSTLIFFAHPKCPCTRASLRQLARLMNDADGKLQAYVVFIKPKDES
                     QEWTETDLRVNAEAIPNVRVLIDEEERETKIFNAQTSGLILLYDRDGNLRFDGGITAS
                     RGHEGDNAGSRAIFEILTEEAVKRAETAVFGCPLQKKDCQGELIEKAQ"
     gene            658..>3283
                     /locus_tag="VGB68_16380"
     CDS             658..>3283
                     /locus_tag="VGB68_16380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0050871.1"
                     /translation="MKSTVKGQAEQIYAGHRQQIYGQTDRMFAVLMAIQWLAGIAAAF
                     FISPRTWIGQTSDVHIHVWAAVFLGGIISFFPIILAILRPGEALTRYTIAAGQMLMSS
                     LLIHLSGGRIETHFHVFGSLAFLSFYRDWRVLVPATAIVALDHMLRGIFYPQSVFGVL
                     STENWRWLEHAAWVIFEDIFLVLSCLRGQREMRQIAENTARLDANEARYRIVTDSASD
                     AIITVDETGTILFINQTVEKIFGYGKEELIGEKFFMLMPKKMRPKLERFMLSGKRNIF
                     TQAVEVTAEHKDGRQFLIEITFGEYDSDGEKVYTAVVRDITDRKQAEEALRKSEEYRN
                     LFKLANDAIIIFEPEGEIILDVNDYACKMYGYPREELIGGSLKNISEQVEKGERQLAG
                     LLNNEAIEPFESVHFQKDGTRINVLINPSMIEFEGKPAVMSINRDVTSRIKADTALRE
                     SEYKLRTLLENMSEGLLHLDENDCIIFTNTRFCEMTGYREEDLAGQNASELFLDEEGR
                     EIFQKSNERSRLGFSENYELPLRTAEGKMIWSLVGVVPTFNEDGKIVGSMSVLTDITE
                     RKLAEAQLLHNAMHDALTGLPNRALFLEHLRRAMGHSPLRKKTFAVLFLDFDGFKLIN
                     DSLGHLEGDALLKLIARRLESLVRADDVVARLGGDEFTILLDELTGSKDALFVVERIK
                     DIFNVPFNLGGREVFISASIGIALRDAKYKTPEDMLRDADIAMYRAKSNGKGQHEIFN
                     KAMREEVSNRLRLETELREALERGEFSVFYQPIMQIAANQLIGFEALVRWFHPQRGQI
                     MPGEFIPIAEETGLILPLGEWILRESCRQVHEWQQRFPARSDLTISVNLSCKQFMQLD
                     LADRVAEILRETGLEARFL"
BASE COUNT          794 a          825 c          902 g          762 t
ORIGIN      
        1 gcaagctttt ctatgagatt cctgcttttt ctttgtttta tattgtggct ttcgaccgct
       61 gttggaggcg ttttttatct ggcgcggtac gaaaatacgc ctgccggtaa gaatttttct
      121 tacccgccgg ctttcccgcc ggaaagtcat attaagcgcg acgtccagcg ctcgacgctt
      181 atcttttttg cgcacccgaa atgtccgtgc acgcgggcga gcctgcgcca gttggcgcgt
      241 ttgatgaacg acgccgacgg caaattgcag gcatatgtag tttttatcaa gccgaaagat
      301 gaaagccaag aatggaccga gaccgatttg cgcgtcaacg ccgaagccat tccgaatgtg
      361 cgcgtattga ttgacgagga ggagcgcgaa acaaaaattt ttaacgcgca gacgtccggg
      421 ttgatactgc tctacgaccg cgacggaaat ctgcggtttg acggaggaat taccgcttcg
      481 cgcgggcacg aaggcgacaa tgcgggaagc cgcgcgattt ttgaaatcct taccgaagaa
      541 gcggttaaac gggcggaaac ggctgttttc ggctgtcctc tgcaaaaaaa agattgtcag
      601 ggcgaattaa tcgaaaaagc acaataaata tgcttcgaat acagctaata aatcaagatg
      661 aaaagcactg ttaaaggaca agccgaacaa atttacgccg ggcatcgaca gcagatttac
      721 gggcagaccg accgtatgtt tgcggttttg atggcgatac agtggctggc gggtatcgcc
      781 gcggcttttt ttatttcgcc gcgcacctgg attggacaaa ccagcgacgt tcacatacac
      841 gtctgggcgg ctgtctttct cggcggcatc atcagttttt ttccgattat actggctatt
      901 ttgcgtcccg gagaggcgct gacgcgctat acgatcgccg ccgggcagat gcttatgtcg
      961 agccttctga ttcatctgtc gggcggtcgc atcgagacgc attttcacgt cttcggctcg
     1021 ctggcttttc tttcgtttta tcgcgattgg cgcgtgctgg ttccggcgac ggcgattgtc
     1081 gcgctcgacc atatgctgcg cggcattttt tatccgcagt cggttttcgg ggttttatcg
     1141 acggaaaact ggcgctggct ggaacacgcg gcgtgggtta ttttcgaaga cattttcctg
     1201 gttttatcgt gcctgcgcgg gcagcgtgag atgcgccaga ttgccgaaaa cacggcgcgg
     1261 ctcgacgcca atgaagcgcg ttaccgcatc gttaccgact cggcttcgga tgcgattatt
     1321 acggtcgatg aaaccggcac gattttgttt atcaatcaaa cggtcgaaaa gattttcggt
     1381 tacggaaagg aagaattaat cggcgaaaag ttttttatgc tgatgccgaa aaaaatgcgc
     1441 ccgaaattgg agcgctttat gctgagcgga aagcgaaata tcttcacgca ggcggtagaa
     1501 gttacggcgg agcacaaaga cggtcgccag tttttaatcg aaatcacctt cggcgagtat
     1561 gattctgacg gagaaaaagt ctataccgcc gtcgtgcgcg acatcaccga ccgcaagcag
     1621 gcggaagaag ccctgagaaa atccgaagaa tacagaaatc ttttcaagct cgccaacgac
     1681 gccataatca tcttcgagcc ggaaggcgaa atcatactcg atgtcaatga ttacgcctgt
     1741 aaaatgtatg gttatccgcg agaggaattg attggcggca gtttgaaaaa catttccgaa
     1801 caggttgaaa aaggagagcg gcagcttgcc ggactattga ataacgaagc catcgaaccg
     1861 tttgaaagcg ttcattttca aaaggacgga acgcgcatta atgtcctgat aaatccttcg
     1921 atgatcgaat ttgaaggaaa gcccgcggtt atgagcatca atcgggacgt gacttcgcgc
     1981 atcaaggcgg acacggcgct gcgcgagagc gaatacaagc ttcgcacact gcttgaaaat
     2041 atgagcgaag gcttgcttca tctggacgaa aacgactgta ttatctttac caacacacga
     2101 ttttgcgaaa tgaccggcta tcgcgaagaa gacttagccg gacagaacgc ttccgaattg
     2161 tttctggacg aagaaggtcg cgaaattttc cagaaatcca acgagcgcag tcgcctcggt
     2221 ttttcggaaa attacgaatt gccgctgcga accgccgagg gcaaaatgat ctggtctctg
     2281 gtcggcgtcg ttccgacctt taacgaagac ggcaaaatcg tcggctcgat gagcgtcctg
     2341 accgacatca cggagcgcaa gctcgccgaa gcgcagctgc tgcacaacgc aatgcacgac
     2401 gcgctgaccg gcttgccgaa ccgcgcgctt tttctcgaac acctgcgccg ggcgatggga
     2461 cactcgccgc tgcggaaaaa gacctttgcc gtcctgtttc tggatttcga cggcttcaaa
     2521 ttaatcaacg attcgctcgg tcatctggaa ggcgacgcgc ttctgaaatt gatcgcccgg
     2581 cggctggaat cgctggtgcg cgccgacgac gtagtggctc ggctcggcgg cgacgaattt
     2641 acaattctgc tcgacgaatt gaccggctcg aaagacgctt tgttcgtcgt cgagagaatc
     2701 aaagacattt tcaacgtgcc tttcaatctc ggcgggcgcg aggtttttat cagcgccagc
     2761 atcgggattg ccctcagaga tgccaaatat aaaacgccgg aagatatgct gcgcgacgcc
     2821 gatattgcga tgtatcgcgc caaatcgaac ggcaaaggac agcacgagat ttttaacaag
     2881 gcgatgcgcg aagaggtcag caatcgcctg cggctcgaaa ccgagctgcg cgaggcgctc
     2941 gaacgcggcg aattttccgt tttctaccag ccgattatgc agattgccgc caatcaacta
     3001 atcggcttcg aggcgctggt tcgctggttt cacccgcagc gcgggcaaat tatgccgggc
     3061 gaatttatcc cgattgccga agaaaccggg ttgattcttc cgctcggcga gtggattctg
     3121 cgcgaaagct gccgtcaggt tcacgagtgg cagcagcgtt ttcccgcccg cagcgacttg
     3181 accatcagcg tcaatctatc ctgtaaacag tttatgcagc tcgatttagc cgaccgcgtg
     3241 gcggaaattc tgcgagaaac cgggctcgaa gcgcgattcc tgc
//
LOCUS       DASWNE010000836         6652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_624028, whole genome shotgun sequence.
ACCESSION   DASWNE010000836 DASWNE010000000
VERSION     DASWNE010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6652
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624028"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..1871)
                     /locus_tag="VGB68_16385"
     CDS             complement(159..1871)
                     /locus_tag="VGB68_16385"
                     /inference="COORDINATES: protein motif:HMM:NF013395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEY0050872.1"
                     /translation="MKKFYFIFAGLILFAVCFGLSYKTSVKAETEDNLVSPNIVISQF
                     QVAGGGSNSANDEFIELHNTSANNVDLNGYRLVYRSAAGTNDVAFVEWTTSTVIAPGA
                     YYLIASTAYDGTVTPNLTYNPTTCSCSMSATGGGLAIRNGALNTGIIIDSVGYGSASN
                     AFIETTPTGAPAANSAQARLNVACQDTDNNANDFLNINPAMPRNSAATPNVCSGGGNS
                     IFASGAANPNPVLPGGTTLFTVSVSPATTPPSTFLTVVANLSQVGGASSQTFFDNGTN
                     GDVTPGDNIFSFQYTIPATIGAGAKIIPFVAADAQGRTASSVITLNVSVPPTTDNPIL
                     FGNPSNATTDVANENNYLMVKPQYTLSYNRSRATANWVAWRLDSSWIGDAPRQDDFRP
                     DPQLPAGWYQVTDQDYSGSGYDRGHMTPSGDRTRSIPDNSATFLMTNIVPQIAANNQG
                     PWEEFESYCRTLAQAGQELYIISGVEGSIGTIAQGRIVVPRNTWKVVIVLPNGSDDLQ
                     RVSKSTRVIAIVVPNHPPLNINAPWRQFRKSVDQVEALTGYNFFSNIPKNTQEIMERR
                     RDVQ"
     gene            complement(2189..5971)
                     /locus_tag="VGB68_16390"
     CDS             complement(2189..5971)
                     /locus_tag="VGB68_16390"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEY0050873.1"
                     /translation="MKKRLALFLAIIAAFGFFAFRQTAESSQLNPVKKQKEFFTELQP
                     QMVQASGFGVSVPVSSLAPAQPETGKTSGKLGRAEQQARAVPNKLPFRKQIAGAPQDE
                     ESNLARVSVEQMPAPALSFNGISSNEVAAAFGFRIIPPDPNGDAGLNHYVQSVNALTR
                     VFDKNGNPLTPPFKLSSLFSVLGTPCSMRDDGDPVVLYDALADRWFLSQFCNNFPPFR
                     QMIAVSKTGDPTGEFYVYEFVMPNVKLNDYPKFGIWTDAIYMSTDEFLGGDYAGSGMF
                     AFDKRKLYAGEPLASYIYFDLASPTTIRFGGLLPSDLDGLNPPPAGAPNIFSGYIADE
                     YGDARDALRLFEFRPNFVNPLASTFTERAESPIAVAPFNPTSPEGRADIQQPPPSEPL
                     DSQSDRLMYRATYRNFGTHDALVLNQTVRVSPVGQTYRAGVRVYELRRNLLSVPPSSP
                     FVVHEQATIGDTAASRWMGSAAQDYQGNIAVGYSYGSEEKKPSITYSGKLASEPAGTF
                     RTERNLIEGTGVQTAFGFRWGDYTAMNIDPTDDCTFWYTNQYYTLESQEESPFGWLTR
                     VGKFKFAECAGAPRATIIGVITNAADGQPIQNATVTASAYSRTTNASGSYGNLLVLPN
                     TYTLTASARGFRSQSVTITVANGQTLIQNFSLQPIAVLENTGTQIAAESCQPNSAVDP
                     GETVTINVALRNTGAQNTTNLTATLLPTGGVTNPGAPQNYGALPVNGQSVSRPFTFTA
                     SAAINCGNPVTLTLQLQDGAENLGTISVVLNTGVLRTALQENFDSVTAPNLPDGWASS
                     ATGAQQNWKTVTNRHQSPPNSAFSPDPNQIGINELVSPAFAVVSPNAEISFRNWYELE
                     TTFLRNKLYDGSVLEIKIGGSGWQDIEAAGGAFLSGGYDGVLDGCCQNPLAGRRAWSG
                     RSGPNQTSEFITSKARLPASAQGQNVQLRWRVGTDLGTFREGQYIDDLVVTDGYVCNC
                     QPAQTNRAPFDFDGDGKTDLSVFRPNDNGGEADFYVQQSGNDSFTGAAWGSVGDLAAN
                     ADYDGDGKTDYAVFRPTANTWFVLQSGNGTFRTQNFGLAGDRLAPADYDGDGKADVAV
                     FRPSDGTWYVSQSSNGQLRATQFGAAEDLPVPADFDADGKADVAVFRPSVGTWFYLQS
                     SNGVFRAAQFGTNGDKPVGGDYDGDGKADFVVFRPSNQTWYLLRSNQGFTAVRFGLGE
                     DRPLQADFDGDGKRDIAVFRPSTGNWFYLRSSNGSFASAQFGLGTDMIVPSIFANF"
     gene            6366..>6652
                     /locus_tag="VGB68_16395"
     CDS             6366..>6652
                     /locus_tag="VGB68_16395"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HEY0050874.1"
                     /translation="MKTKKIFRPLSGWNLLLIVSLVSVLSIIILSSINFPAAEAAGSI
                     TGRVFHDYNSNGAYDTTSGLNSIDRGVTGVTVTAYDAAGVSRGTTTTNASG"
BASE COUNT         1515 a         1804 c         1819 g         1514 t
ORIGIN      
        1 aaaacggaaa aaagattttt cataactcaa aatatttttt tacgggcaag ttaaaagaga
       61 aaaatgggac gtccgatatt attaagatgt gtcaaaagta tttaaggttt atcttaaata
      121 ctctcgggca atcttaaaaa aacggcgtcc cgcgtctttt actgcacgtc tctgcgtctt
      181 tccataattt cctgcgtatt tttcggaatg tttgaaaaga agttataccc ggtcaaagcc
      241 tcgacctggt cgacgctctt gcggaactgc cgccacggcg cgttgatatt cagcggcgga
      301 tgattcggaa cgacgatggc aatcacgcgc gtcgatttgc tgacgcgctg caaatcgtcg
      361 cttccgttcg gcagaacaat cacgactttc caggtgttgc gcggcacgac gattctgccc
      421 tgcgcaatcg tcccgatgct gccttcgacg cccgaaatta tataaagctc ctgcccggct
      481 tgcgccagcg tgcggcagta actttcaaat tcttcccacg gtccctgatt gttcgccgcg
      541 atttgcggca cgatgttcgt catcagaaac gtcgccgaat tgtcgggaat cgagcgcgtc
      601 cggtcgcccg aaggcgtcat atgaccgcgg tcgtaacccg aaccggaata atcctggtca
      661 gtcacctgat accagccagc gggcaattgc gggtcgggac gaaaatcgtc ctgacgcggc
      721 gcgtcgccaa tccagctcga atccaaacgc cacgccaccc aatttgccgt cgcgcggctg
      781 cggttataag agagcgtgta ttgcggcttg accatcaaat aattgttttc gttcgccacg
      841 tccgtcgtcg cattgctcgg attgccgaac aaaatcggat tgtccgtagt cggcgggacg
      901 gagacgttca gcgtaatgac cgagcttgcc gtgcgtccct gcgcgtcggc ggcgacaaaa
      961 ggaatgattt tcgcgcccgc tccgatggtc gccggaatcg tgtattggaa tgaaaatata
     1021 ttgtcgccgg gcgtcacgtc gccgttcgtg ccgttgtcga aaaacgtttg cgaagacgcg
     1081 ccgccgacct gcgataaatt agcgaccacg gtcagaaaag tgctcggagg cgtcgtcgcc
     1141 ggagaaaccg aaacggtaaa caaggtcgtc ccgccgggca gaaccgggtt cggattagcc
     1201 gcgccgctgg cgaaaatcga gttgccgccg cccgagcaaa cgttcggcgt cgcggcgctg
     1261 ttgcgcggca tcgccgggtt gatgttcaaa aaatcgttgg cgttgttgtc cgtgtcctgg
     1321 caggcgacat tcagccgcgc ctgagcggag ttggcggcag gtgcgccggt cggcgtcgtt
     1381 tcgataaaag cgttgctcgc cgaaccgtaa ccgaccgaat ctataattat tcccgtgttc
     1441 agcgcgccgt tgcgaatggc aagcccgccg cccgtcgccg acatcgaaca cgaacaggtc
     1501 gtcggattgt aggtgagatt cggcgtgacc gtgccgtcat aggcggtcga ggcaatcagg
     1561 taataagcgc ccggcgcaat caccgtgctg gtcgtccatt cgacaaacgc gacgtcgttc
     1621 gttccggcgg cagagcgata aaccaatctg tagccgttca ggtcgacgtt gtttgcgctg
     1681 gtgttatgca gctcgataaa ttcgtcgttc gcggagttgc tgccgccgcc ggcgacctga
     1741 aactggctga tgacgatgtt cggcgaaacc agattatctt cggtttcggc tttaacgctt
     1801 gttttgtaag aaagtccgaa acagactgca aacagaatca gaccggcaaa aataaaataa
     1861 aacttcttca ttagaataaa aacttccttt cgattttact gacagggcgg gcttattaac
     1921 ggcaagtaac ctttgtaaca caatttgccg caaaatgtaa tttgaacggt cgcaaaaagg
     1981 atttcagccg ccgccggaca ctgaggttct ttagagaaat taaccgcaga ttgacgcgaa
     2041 taaacgcgga tagaattaat ttgcaatttc aaatttgcga taacaaaatc gcaatctttg
     2101 cttgtcaatc cgcgtttatt cgcgtcaatc tgcggcttct agttcttaaa atcccagtat
     2161 cctgtgtgcc ctctgcggct gaaatttctt aaaaattagc aaaaatcgac ggaacaatca
     2221 tatccgtgcc cagaccgaac tgcgcgctcg caaaactgcc gtttgagctt cgcaggtaaa
     2281 accagttacc cgtgcttgga cgaaaaaccg cgatgtcgcg tttgccgtct ccgtcaaaat
     2341 ccgcctgcaa gggtctgtct tcgccgagcc cgaaccggac ggcggtaaat ccctgattgc
     2401 ttcgaagcaa ataccacgtc tggtttgacg ggcgaaaaac gacgaaatcg gctttgccgt
     2461 cgccgtcgta atcgccgccg accggcttat cgccgtttgt cccgaattgc gcggcacgaa
     2521 aaacgccgtt cgagctttgc agataaaacc acgtgccgac ggacgggcga aagaccgcta
     2581 catccgcttt tccgtccgca tcgaagtctg ccggaacagg caaatcttcc gccgctccaa
     2641 actgcgtcgc gcgtaactga ccgttcgagc tttgcgaaac ataccaggtt ccgtctgacg
     2701 gtcgaaaaac agcaacatcc gctttgccgt cgccgtcata gtcggcgggc gcgagacggt
     2761 cgcctgccag accgaaattc tgcgtgcgaa acgttccgtt tccgctttgt aaaacgaacc
     2821 acgtgtttgc cgtcggacgg aaaacggcat aatcggtttt gccgtcgccg tcgtaatcgg
     2881 cgtttgcggc gaggtcgccg acgcttcccc acgccgcgcc ggtaaacgaa tcgtttccgc
     2941 tttgctgaac gtaaaaatca gcctcgccgc cgttgtcgtt cgggcgaaaa acgctcaaat
     3001 cggttttgcc gtcgccgtca aaatcaaacg gcgcgcgatt cgtctgcgcg ggctggcagt
     3061 tgcagacgta accgtccgtc acgaccagat cgtcgatata ctgaccttcg cggaaagttc
     3121 cgaggtcggt gccgacgcgc cagcgcagct ggacgttttg cccttgcgcc gaggcgggca
     3181 gcctggcttt cgaggtgata aactccgacg tttgattggg tccgcttctg cccgaccagg
     3241 cgcgtctgcc cgcgagcgga ttctggcagc agccgtctaa aacgccgtcg taaccgccgg
     3301 atagaaacgc gccgccagcc gcttcgatgt cctgccagcc gctgccgccg atttttattt
     3361 ccagaaccga gccgtcgtaa agcttattgc gcaaaaacgt cgtttccagc tcataccagt
     3421 tgcggaaact gatttcggca ttcggcgaga cgactgcaaa cgcgggcgaa acaagctcgt
     3481 taatgccgat ttgattcggg tcgggcgaaa aggcggaatt cggcgggctt tgatggcgat
     3541 tggttacggt tttccaattt tgctgcgctc cggtcgcgct gctcgcccag ccgtccggca
     3601 aattcggcgc ggttacggaa tcgaaatttt cctgcaaagc ggtgcgcaga acgcccgtat
     3661 tcaaaactac cgaaatcgtg ccgagatttt ccgcgccgtc ctgcaattgc aaagtcaaag
     3721 tcacgggatt gccgcaattt atagccgcgc tcgccgtgaa agtgaacggg cgcgaaacgc
     3781 tctgtccatt gactggcagc gcgccgtaat tttgcggcgc gccgggattc gtgacgccgc
     3841 cggtcggtaa aagcgtggcg gttaaatttg tcgtgttctg cgcgccggtg ttgcgaagcg
     3901 cgacgtttat cgtcacggtt tcgccggggt cgacggcgct gttcggctgg cagctctccg
     3961 ccgcgatttg cgttccggta ttttccagta cggcgatcgg ctgcagcgaa aaattctgta
     4021 tcaaggtttg cccgttcgcc accgtaatcg taacgctctg tgaacgaaag cctctggcgg
     4081 aagcggttag cgtgtaagta tttggtaaaa ccaataaatt gccgtagctg ccgctcgcgt
     4141 ttgtggttct ggaataagcc gaagccgtga cggttgcgtt ttgaatcggc tgaccgtcgg
     4201 cagcgttggt aataacgccg ataatcgtcg cgcgcggcgc gcccgcgcat tcggcgaatt
     4261 tgaattttcc gacgcgcgtc agccagccga aaggactttc ttcctgactt tccagagtgt
     4321 aatactgatt cgtataccag aatgtgcagt cgtcggtcgg gtcgatgttc atcgcggtgt
     4381 aatcgcccca gcgaaaaccg aaagccgtct gcacgcccgt gccttcaatc aaatttctct
     4441 ccgtgcgaaa cgtccccgcc ggctcgctcg ccagtttgcc cgaataggta atcgaaggtt
     4501 ttttttcttc gctgccgtaa ctgtagccga cggcgatatt gccctgataa tcctgcgccg
     4561 cgctgcccat ccagcggctc gcggctgtat ctccgatggt tgcctgttcg tgaacgacga
     4621 aaggcgagga cggcggaacg gaaagcagat tccggcgcag ttcgtaaacg cgaactccgg
     4681 cgcgataagt ctgaccgacg ggcgaaacgc gcaccgtctg atttaaaacc agcgcgtcgt
     4741 gcgtgccgaa atttctgtat gtcgcgcgat acatcaggcg gtcgctttgc gaatcgagcg
     4801 gctcgctcgg cggcggttgc tgaatatcgg cgcgaccttc ggggctggtc gggttaaaag
     4861 gcgcgacggc tatcgggctt tcggcgcgtt cggtaaaggt cgaagcgaga ggattgacaa
     4921 aattcgggcg aaactcgaaa agccggagcg cgtcgcgcgc gtcgccgtat tcgtcggcga
     4981 tgtaaccgga gaaaatattc ggcgcgccgg cgggcggcgg attcagcccg tccaggtcgg
     5041 aaggcaaaag cccgccgaag cgaatcgtcg tcggcgaagc caaatcgaag taaatataac
     5101 tcgccagagg ctcgcccgcg tagagcttcc gcttatcgaa ggcaaacatt ccgctgcccg
     5161 cgtaatcgcc gcccaagaat tcgtccgtcg acatataaat cgcgtccgtc cagatgccga
     5221 atttcgggta atcgttgagc ttgacgttcg gcatcacgaa ttcgtaaacg taaaactcgc
     5281 cggtcgggtc gcccgttttt gaaaccgcaa tcatctggcg aaaaggcgga aaattattgc
     5341 agaactggct cagaaaccat cggtcggcga gcgcgtcgta caaaacgacc gggtcgccgt
     5401 cgtcgcgcat cgagcaaggc gtgccgagaa cggaaaacaa gctgctcaat ttaaaaggcg
     5461 gcgtcagcgg attgccgttt ttatcaaaaa cgcgggtcag cgcgttgacc gattggacgt
     5521 agtgatttaa accggcgtcg ccgttcgggt cgggcggaat gatgcgaaat ccgaaagcgg
     5581 cggcgacttc attgctggaa attccgttga aagacagcgc aggcgcgggc atttgttcga
     5641 ccgaaactcg cgctaaattc gattcctcgt cctgcggcgc gccggcgatt tgtttgcgaa
     5701 acggcagttt gttcggcacg gcgcgcgcct gctgttcggc gcgtccgagc ttgccgctgg
     5761 tttttcccgt ttcgggctgc gcgggcgcta aactgctgac cggaacggag acgccgaaac
     5821 cggaagcctg caccatctgt ggctgaagct cggtgaaaaa ttctttttgc tttttgaccg
     5881 gatttagctg tgagctttcc gccgtttgcc tgaaagcgaa gaaaccaaac gccgcaatga
     5941 tggctaagaa taacgccagc cgttttttca tgattaaacc ttttcgtgaa gaaattttaa
     6001 gacgatgata acagaatctc tttctaacct aaaaaaaaat ccgcgatttc acaaattaat
     6061 tcagcgtttt tccgcgtttt aaaccggatt tttttaatat agccggctgt tttgaaaaac
     6121 attgttaaaa acagttgaaa aaaacattta aagctgttac tttattatct ggttaagtgc
     6181 tgcccgcaga cgattaccac aaccacgcca gagccaagct cttcaaagct ctgtaaatct
     6241 accgaccaaa atctaaaaaa taaaacaata cccggaattg cggatacagt ttgcgattgt
     6301 ttgtgcgtca ggaagtttca gacttcattt ctaaattcgc cgttccgcgt ccaaaaagat
     6361 aaaagatgaa aactaaaaaa atcttccgcc ctttatccgg ctggaatctg ttacttatcg
     6421 tcagtctcgt tagcgtactt tcaataataa ttttatcgag cattaatttt ccggctgccg
     6481 aagcggcggg aagcatcacc ggcagagtat ttcacgatta caactcgaac ggcgcttatg
     6541 atacgacgag cggattaaac tcgatagacc gcggcgtaac cggcgtgacg gtcacggcgt
     6601 acgacgcggc tggcgtctcg cgcggcacga cgacgaccaa tgcgagcggc aa
//
LOCUS       DASWNE010000837         2473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_98469, whole genome shotgun sequence.
ACCESSION   DASWNE010000837 DASWNE010000000
VERSION     DASWNE010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98469"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1191)
                     /locus_tag="VGB68_16400"
     CDS             complement(<1..1191)
                     /locus_tag="VGB68_16400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050875.1"
                     /translation="MKKSLFRHALPAFVIGLFFIFNQSIDAQSPLDGFDPNANGTVRA
                     VTVQPDGKILIGGDFTTLAPNDGAPVTRNRFARLNPDGTLDAAFNTSFNGSVYSITVQ
                     PDGKILVGGAFGSVTPNGGAPFSCGDIVRLNANGTVDTIFNPRADGFVYTIVVQPDGR
                     ILIGGYFATLSPNGGAQVVRFRFARLDPDGTVDAAFAPNPNNSVQAIALQADGKIIIA
                     GYFNLLAPEGGGTIAVNKIIRFNVDGSLDTSFDPNPNNGVRAMAVQSDGRILIAGNFT
                     TLAPNGGAPVARNCFARLNPDGTLDATFDPNVSGGSLIVYSIALQADGKILIGGEFSR
                     LAPNGGASVTRFNIARVNADGTVDAIFDPRVDHIVNAVAVQPDGKILIGGYFRFLAPS
                     GGASV"
     gene            complement(1188..>2473)
                     /locus_tag="VGB68_16405"
     CDS             complement(1188..>2473)
                     /locus_tag="VGB68_16405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050876.1"
                     /translation="NMTQPEFVLSGGFTGAGANRLNANHSYDTAFRTLIGIRGNVLNI
                     APLTNGQIVIGGDFNAVNRTIRRPGISRLTAAGILDTRFDPAFIGMFYYSFVRSVTPL
                     ADNRVFAGGLFLERLNLDGSEDPTFNASIPSAVRFNDIAVQPDGKVIAVGQSIPYQGD
                     TCCVLRFNADGSLDSSFQFPGLGGASVKKVLIQPDGKILIGGQFSRIGYSARSSIARL
                     DADGSLDTSFNPVGGASGIVADIALQPDGKVLISGEFSQVNGVGRQGIARLNADGSLD
                     ATFDAAANSPVFTVELQTDGKILVGGAFTSIGGAARNRIARLNPNGSIDSTFDVGAGA
                     DGNVHVIALDTSGRVLAGGEFVRYDNTPKAGIVRLLNDTTAPRATPFEYDAKSGQVKN
                     YDVGDEFSLFAPGQQNLRLHYREYPENIIYPETIK"
BASE COUNT          562 a          705 c          699 g          507 t
ORIGIN      
        1 aactgacgcg ccgccgctcg gcgcgaggaa acggaaatag ccgccgatta aaatcttgcc
       61 gtccggctgc accgcgacgg cattgacaat gtggtcgaca cgcgggtcga aaatcgcatc
      121 aaccgtgccg tcggcgttca cgcgggcgat attgaagcga gtaacggacg cgccgccgtt
      181 cggcgcgagc ctcgaaaact cgccgccgat aagaatcttg ccgtccgcct gcaacgctat
      241 tgaatagaca attaaagagc cgccgctgac attcgggtcg aaagtcgcgt cgagcgtgcc
      301 gtccgggtta agccgggcaa aacaattgcg cgcgaccggc gcgccgccgt tcggcgcaag
      361 cgttgtgaaa ttaccggcaa tgagaatcct gccgtccgac tgcacagcca ttgcccggac
      421 gccgttgttg ggattcgggt cgaaactcgt atcgagcgag ccgtcgacgt tgaagcgaat
      481 gattttattg acggcaatcg ttccgccgcc ttccggcgcg agcaggttaa aataaccggc
      541 gataataatc ttgccgtccg cctgcaacgc gattgcctgg acgctgttgt tgggattcgg
      601 ggcgaaagcc gcatcgaccg tgccgtccgg gtcaaggcgg gcgaaacgaa agcgaacaac
      661 ctgcgcgccg ccattcggcg aaagcgtagc gaaatagccg ccgattaaaa tcctgccgtc
      721 cggctggacg acgattgtat agacgaagcc gtcagcgcgc ggattaaaaa tagtatcgac
      781 cgtgccgttg gcgttcaggc ggacaatatc gccgcaggaa aagggcgcgc cgccattcgg
      841 cgtaaccgaa ccgaatgcgc cgccaactaa aattttgccg tccggctgta ccgtgattga
      901 ataaacacta ccgttgaaac tcgtattaaa agccgcgtcg agcgtgccgt ccgggttcaa
      961 tcgggcaaaa cgattgcgcg tcaccggcgc gccgtcgttc ggcgcgagcg ttgtaaaatc
     1021 gccgccgatt aaaatctttc cgtccggctg gacggtaacg gcgcgaaccg tcccgttggc
     1081 attcgggtcg aaaccgtcca gcggcgattg agcgtcgatt gattgattga agataaaaaa
     1141 taagccgatt acaaaagcgg gtagagcgtg ccgaaataag gattttttca tttgattgtc
     1201 tccgggtaaa ttatattttc aggatattcg cgataatgaa ggcgaaggtt ttgttgaccg
     1261 ggagcaaaca aagaaaactc atctccgaca tcataatttt ttacttgtcc gctctttgcg
     1321 tcgtactcaa acggcgtcgc ccgcggcgct gtggtgtcgt tcaacaggcg gacgatacct
     1381 gctttcggcg tattgtcgta tctgacaaac tcgccgccgg ctaaaactct gccgcttgtg
     1441 tccagcgcaa tcacgtggac gttgccgtcc gctcccgctc cgacgtcgaa ggtcgaatcg
     1501 attgagccgt tcggattcag acgagcgatg cggtttcgcg ccgcgccgcc gatactggta
     1561 aacgcgccgc cgaccagaat ttttccgtcc gtctgaagct cgaccgtgaa aaccggcgaa
     1621 ttcgccgccg cgtcaaaagt cgcatccagc gacccgtccg cgttcagacg ggcgatgccc
     1681 tgtctgccga caccgttgac ttgagaaaac tcgccgctta tcagcacttt tccgtccggt
     1741 tgcagggcga tgtcagcgac gataccgctt gctccgccca cagggttaaa cgacgtatcg
     1801 agcgagccgt ccgcgtctaa tcgcgcgatg ctgctgcgcg ccgaatagcc aatgcgggaa
     1861 aactgtccgc cgataagtat tttcccgtcg ggctgaatga gaactttttt gaccgaagcg
     1921 cctcccagcc cgggaaattg gaacgaggaa tcgagcgagc cgtccgcgtt aaatctcagc
     1981 acacaacagg tatcaccctg gtacggaatt gattgaccga cggcgataac tttgccgtcc
     2041 ggctgcaccg caatatcgtt gaaacgtacc gcagatggaa tgcttgcatt aaaagtcggg
     2101 tcttccgagc cgtccagatt caaccgctcc agaaacagtc cgccggcgaa aaccctgttg
     2161 tccgccagcg gcgtcaccga acgaacgaac gagtaataaa acatgccgat aaacgccggg
     2221 tcaaaccttg tgtcgagaat gcccgcggcg gtcaggcgcg agatgcccgg acgacgaatc
     2281 gttcggttga ccgcattaaa atctccgccg ataacgattt gcccgttcgt gagcggcgca
     2341 atgttcagga cgtttccgcg aattcctatc agcgttcgga aagcggtgtc gtatgagtgg
     2401 ttagcgttta gtcgatttgc ccccgcgcct gtaaatcctc ctgacaatac aaattcgggc
     2461 tgggtcatat tgc
//
LOCUS       DASWNE010000838         2204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_521378, whole genome shotgun sequence.
ACCESSION   DASWNE010000838 DASWNE010000000
VERSION     DASWNE010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521378"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1105
                     /locus_tag="VGB68_16410"
     CDS             <1..1105
                     /locus_tag="VGB68_16410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0050877.1"
                     /translation="KIYRMIWSGMARKFFLIILLLIFAAAAAFGQSGRIKPTPEELPS
                     KDRERIIYVPTQLPNPKAKPTPTPTPKTNDDDDGEIIRVQSALVAIPVSVTDPQGRAV
                     TALKLEDFLLEIDGKASEISELSRSSNTPVRLALLFDNSSSVRIAREFEKKAAIRFFR
                     QVIRPEKDLVALFSVASTTELEQPLTRDVSSLVRAIELFAEPAGGTQLLDGIIQAAQY
                     LNSTEGRRIIVIVSDGEDNLSDSTLEQTVRAAQLNNCQIYVVKTTDFENIKRTGARGG
                     NANTIFLSAERRMQELARQTGGAVYSPVDEKELDAAFTRISAELSQQYILNYYPAEDE
                     KDKRGEFRAISLKVKNRENLTVRTRKGYYVPKK"
     gene            1257..2171
                     /gene="xerC"
                     /locus_tag="VGB68_16415"
     CDS             1257..2171
                     /gene="xerC"
                     /locus_tag="VGB68_16415"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEY0050878.1"
                     /translation="MLEEYLRQFLEHLRYERNVSAHTLRNYSSDLEQFREYLVTIGKT
                     GDIAVSQIDHLTIREWMASLHGLNKKKTSIARKLASLRTFFQFLVRESVLETNPAKLV
                     STPRVERKLPNHLSMEDAVRFIETPDLQTDLGKRDRAILEFLYATGVRVGELVNLNMK
                     DIDFRERLARVTGKRKKQRILPFGEPSLQALMLYLSETRPIFLNNCPPSERDDQAVFL
                     NYQGTRITTRSVGRMVDKYIKLCADIHDISPHSLRHSFATHLLDSGADLRDIQELLGH
                     ARLSTTQIYTHVSMEKLIEVYDRAHPKA"
BASE COUNT          558 a          572 c          579 g          495 t
ORIGIN      
        1 gaaaatttac cggatgattt ggagtggtat ggctcgtaaa ttttttctta taatattgct
       61 tctgattttt gcggcggcgg cggctttcgg gcagagcggg cggattaaac cgacgccgga
      121 agaattgcct tcgaaagacc gcgagcgcat catttacgtt ccgacgcagc tgccgaatcc
      181 gaaagccaaa ccgacgccga ccccgacgcc gaaaacaaat gatgacgatg acggcgaaat
      241 catccgcgtt caatccgcgc tggtcgcgat tccggtttcc gtaaccgacc cgcagggacg
      301 cgccgtgacc gctcttaagc tcgaagattt tctgctggaa atcgacggca aagcgtcgga
      361 aatcagcgag ctttcgcgct cgtccaatac gcccgtgcgg ctcgcgcttt tgtttgacaa
      421 cagctcgagc gtccgcatcg cgcgcgaatt cgagaaaaaa gccgcgattc gctttttccg
      481 gcaggtgatt cgtccggaaa aggatttggt cgcgctgttc agcgtcgcct cgacgaccga
      541 gcttgaacag ccgctgacca gagatgtttc ctcgctcgtc agagccatcg agcttttcgc
      601 cgaaccggcg ggcggaacgc agcttctgga cggcatcatt caggcggcgc aatatttaaa
      661 ttccaccgaa gggcggcgca ttatcgtcat cgtttcggac ggcgaggata atctgagcga
      721 ttcgaccctg gagcagacgg ttcgcgccgc gcagcttaat aactgccaga tttacgtggt
      781 gaaaacgacc gattttgaaa acatcaagcg gacgggcgcg cgcggcggaa atgccaacac
      841 catttttctt tcagccgagc gccgaatgca ggaactcgcc agacagacgg gcggcgcggt
      901 ttattcgccg gttgacgaaa aggagctgga cgcggcgttt acgcgcattt ccgccgagct
      961 ttcccaacaa tatatcctga attattatcc agccgaagac gaaaaagaca agcgcggcga
     1021 atttcgcgcg atttcgctga aagtcaaaaa ccgcgaaaac ctgaccgttc gcaccagaaa
     1081 aggttattac gttccgaaga agtgaaaacc ggcaatcgaa aatcgtgaat cgtagatttc
     1141 gaaaatcgaa aaccgcgaat cgccgtttgg ttttagctga aatatgttaa attctaaacg
     1201 gtttttctat tcccgattgc cggttttcga ttttcgaaaa tcacgattca cgaaatatgc
     1261 ttgaagaata tctgcgacag tttctcgaac atctgcgtta cgagcgcaat gtcagcgcgc
     1321 acacactgcg aaattattcc agcgatttgg agcagtttcg cgaatatctg gtcactatcg
     1381 gaaaaaccgg cgacatagcc gtcagccaga ttgaccatct gacgattcgc gaatggatgg
     1441 cttcgctgca cgggctcaac aaaaagaaaa cttcgattgc gcgcaagctc gcctctttaa
     1501 gaaccttttt tcaatttctg gtgcgcgaaa gcgttctgga aacaaatccc gccaaattgg
     1561 tctcgacgcc gcgagtcgaa agaaagctgc cgaatcatct ttcgatggaa gacgccgtgc
     1621 gctttatcga aacgccggat ttgcagacgg atttagggaa acgcgaccgg gcgattttag
     1681 aatttctcta tgcgacgggc gtgcgcgtcg gcgaactggt caatctgaat atgaaggata
     1741 tagattttcg cgagcggctg gcgcgcgtga ccggaaagcg caaaaaacag agaattctgc
     1801 ctttcggcga gccgtcgcta caggctttaa tgctttatct ttcggaaacg cgcccgattt
     1861 ttctaaacaa ctgcccgcct tcggagcgcg acgaccaggc ggttttctta aattatcagg
     1921 gaacgcgcat cacgacgcgc tcggtcgggc gaatggtcga taaatacatc aaattgtgcg
     1981 ccgacattca cgacatttcg ccgcactctc tacgacattc ctttgcgacg cacctgctgg
     2041 attccggcgc ggatttgcgc gatattcagg agcttctggg acacgcgcgg ctttcgacga
     2101 cgcagattta cacgcacgtt tcgatggaaa aactgatcga agtttacgac cgtgcgcacc
     2161 cgaaagcgtg ataaaatatc cgaataaaag aacgaaaaat atgg
//
LOCUS       DASWNE010000839         5228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_28221, whole genome shotgun sequence.
ACCESSION   DASWNE010000839 DASWNE010000000
VERSION     DASWNE010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28221"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /gene="folP"
                     /locus_tag="VGB68_16420"
     CDS             <1..865
                     /gene="folP"
                     /locus_tag="VGB68_16420"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY0050879.1"
                     /translation="FFTFSLSMYWQTARRRLSLEKTILMAILNVTPDSFSDGGEFFSI
                     DDALRQAEKSIEAGADILDIGGESTRPASKRVSSVEEIRRVVPVIEAVAKRFDIPISI
                     DTSKSEVAEKSIEAGAEIINDVSGLRFDERIAQIAAAKNAGLVLMHLRGDFETMHRQK
                     PVADIFREVSEGFRRSIEKAESFNVKKENIALDVGIGFGKTFNQNLELLAKLDKLIKE
                     FPEFPMLVGTSRKSFIGKILGDVPAAERLNGSLASAAIAVWNGAKIVRVHDVKATVEA
                     LKVVEAIKKEL"
     gene            862..1413
                     /locus_tag="VGB68_16425"
     CDS             862..1413
                     /locus_tag="VGB68_16425"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HEY0050880.1"
                     /translation="MKFIRFTAIVLFLLAVSGFTECYKPVTNSGLPKRIKKVAVPAFQ
                     FEARGLRYRVESRFTEAVTKEIIRRGRGLKVQGSREGADAVVEGTIRDFNFTGVLLDR
                     EGRARIYEVTIVVAVTVRDLQENKILYDNQNFIFRDSFEFSEDPRSFFNEEDPAVERM
                     SRAFAESVVSSVVNGLGVKEEKK"
     gene            1446..2471
                     /gene="holA"
                     /locus_tag="VGB68_16430"
     CDS             1446..2471
                     /gene="holA"
                     /locus_tag="VGB68_16430"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEY0050881.1"
                     /translation="MKILSREDLRNQLIRGEIAPVYLLFGDETYLRDLAAKIIAERAL
                     NDSGLREFNETAFSLADSKIDYALSAAEQMPMISSRRVVTITEVVVSGTGKKDSLKEE
                     DEAVLMRYLSRPSETSVVIFVADELDKRRKISKLLLENCLSVEFTPLSDNELLDEVKK
                     QLKKTNAQIEERAARYLVGLVGNNLQKLMTEAEKLATAALPDSLITFELVEQLVPNSR
                     ELSNFELTDYLLAKNRTRSLQILHKILDDGAEPLMLLGLIANNFRRLFLAKELMRSGV
                     DRTEVARTMRLPFSKQEEFLATARRADTERLSWNLRRIAQADLAIKTSQATPRLQIEM
                     LVCELTK"
     gene            complement(2680..2946)
                     /gene="rpsT"
                     /locus_tag="VGB68_16435"
     CDS             complement(2680..2946)
                     /gene="rpsT"
                     /locus_tag="VGB68_16435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEY0050882.1"
                     /translation="MPNHKSAEKRVRQNEKRRLVNRNNRSSLRTQIKKLRTAIAGSDN
                     QQSQELLVPTISAIDKAVNKGILHKNTAARYKARLTGHVNKAVQ"
     gene            complement(3405..4217)
                     /locus_tag="VGB68_16440"
     CDS             complement(3405..4217)
                     /locus_tag="VGB68_16440"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEY0050883.1"
                     /translation="MVDYYKVLKISPKASTAEIKSAYRRLARKMHPDVNGGTEEATRE
                     FALIAKSYEILSNAQERAHYDKQYFKIFHNGSLHDSDSVMYSDNSHASRMRQIAFENR
                     YNRIVDSIIDAERKEVLALQKVIFPIVALFLSTCFVAIFKPKFWSNSEIIGKIVLLTL
                     FITGVIHLVNRLRAGFDHYTYNNKLHDSILEETEEETRPYSRLTAVAFLLIGIGISLV
                     VGMVIGNYLEMFIAASMPKLFSPTLRLEFIFYPPIVVLLVDAMHGVASKVDV"
     gene            complement(4545..5009)
                     /locus_tag="VGB68_16445"
     CDS             complement(4545..5009)
                     /locus_tag="VGB68_16445"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY0050884.1"
                     /translation="MSEAKPNINVTPLIDVLLVLLIIFMVISPSKPSTFKAKVPQETK
                     ADKLPPHPHTLIVTINADSSLKLNQEDSGSVGAPQSLTEKLSAKFEERSQSGIYDENL
                     VARNDLPNEEKILKTVFIKAPKNFSYGEVAKVVDAVKLSGANPVGLQIDGLD"
BASE COUNT         1406 a         1205 c         1300 g         1317 t
ORIGIN      
        1 ctttttcact ttttcacttt ctatgtactg gcagaccgcg cgccgcagat tgtcgttgga
       61 aaaaactatc ctgatggcga ttttaaacgt cacgccggat agtttttccg acggcggcga
      121 gtttttttcg attgacgatg ctttgagaca agccgaaaaa tcgattgaag cgggcgcgga
      181 cattctcgac atcggcggcg aaagcacgcg accggcgagc aagcgagttt cgtccgtcga
      241 agaaatccga cgcgtcgtgc ccgtgattga agcggtcgct aaaagatttg atattccgat
      301 ttcgattgat acaagcaaat ccgaagtcgc cgaaaaatcg attgaagcgg gcgcggaaat
      361 catcaacgac gtttccggct tgcggtttga tgagagaatc gcacagattg cggcggcgaa
      421 aaacgcgggt ttggttttaa tgcatctgcg cggcgatttt gaaacgatgc accggcaaaa
      481 gccggtcgcc gatattttca gggaagtttc cgaaggcttt cgccggagca tcgaaaaagc
      541 cgaaagcttt aatgttaaaa aagaaaatat agctctggac gtcggcatcg gattcggcaa
      601 aacttttaat cagaacttag agcttttggc aaaattggat aaacttataa aagagtttcc
      661 cgaatttccg atgctcgtcg gcacgtctcg aaaatcgttt atcgggaaaa ttttaggcga
      721 tgttcccgca gccgaaagat tgaacggaag cctggcgagc gcggcgatcg ccgtttggaa
      781 cggcgcgaaa atcgtccgcg ttcacgacgt aaaagcgacg gtcgaagccc tgaaagtcgt
      841 cgaggcgatc aaaaaggaat tatgaaattc attcgtttta cagcaattgt tttattttta
      901 ttagcggttt ccggctttac cgaatgctac aagccggtga cgaattccgg tctgccgaaa
      961 cgcattaaaa aggtcgccgt gccggcgttt caattcgaag cgcgcggctt gcgttaccgc
     1021 gtcgaatcgc gttttaccga agccgtgaca aaggaaatta ttcgtcgcgg tcgcggcttg
     1081 aaagtgcagg gaagccgcga aggcgcggac gccgtggtcg aaggcacgat tcgggatttt
     1141 aattttacgg gcgttctgct cgaccgcgaa ggtcgcgcgc gcatttatga agtgacgatt
     1201 gtcgtcgcgg tgacggtcag ggatttgcag gaaaataaaa ttttgtacga caatcaaaat
     1261 tttattttcc gcgattcgtt cgagttttcc gaagacccgc gctcgttttt caacgaggaa
     1321 gacccggcgg tcgagcgaat gtcgcgcgcc tttgccgaat cggtcgtctc gtcggtcgtc
     1381 aacggcttgg gcgtgaaaga agagaaaaaa taattgcgcc ggagaatagt gccagtcgtt
     1441 attttatgaa aattttgagc cgcgaagatt tacgcaatca gttaatcagg ggcgaaatcg
     1501 cgcccgttta cctgcttttc ggcgatgaaa cttacctgcg cgatttggcg gcgaaaatta
     1561 tcgccgagag agccttaaac gattcgggct tgcgcgaatt taacgaaacc gctttcagcc
     1621 tcgccgattc gaaaatagat tacgcgcttt ccgccgccga gcagatgccg atgatttcct
     1681 cgcgccgcgt cgtcacaatc acggaagtcg tcgtttccgg aacgggcaaa aaagacagtt
     1741 taaaggaaga agacgaagcg gttttaatgc gctatctttc gcgcccgtcg gaaacgagcg
     1801 tcgtgatttt tgttgccgac gagctggaca agcgccgtaa aatttccaaa ttgctgctgg
     1861 aaaattgcct ctcggtcgaa tttacgccgc tttccgacaa tgaattgctc gatgaagtaa
     1921 aaaaacagct taaaaaaacc aatgctcaaa tcgaagaacg cgcggcgcgc tatctagtcg
     1981 gcttggtcgg gaataatctg caaaaattaa tgacggaagc cgaaaagctg gcgaccgccg
     2041 cattgcccga ttcgctgatt acgtttgagc tggtcgaaca gttagtgccg aattcgcgcg
     2101 agctttcgaa tttcgagctg acggattatc tgctggcgaa aaaccgcacg cggtctttgc
     2161 aaatactgca caaaatcctc gacgacggcg cggagccctt gatgcttctg ggtttgattg
     2221 cgaataattt ccggaggctt tttctggcga aggaattgat gcgcagcggc gtcgaccgca
     2281 ccgaggtggc gcgcacgatg cggctgcctt tcagcaaaca ggaagaattt ctggcgaccg
     2341 cgcgccgcgc cgatacggaa aggctttcgt ggaatctgcg gcgcatcgcg caagccgatt
     2401 tagctattaa aacttcgcag gcgacgccgc gcctgcaaat cgaaatgctg gtctgcgaat
     2461 tgactaaata gccccaaaaa gacgccggaa acgcgaaagg gaaatcagga aataccgggc
     2521 gcgaataaca cgaattaatt ttatttcttt tattcgtgat tactcgcggc taaatctttt
     2581 cgtggttatt cgtagttaaa tgaaaaaaga cagaagcgag ttttacgtcg ctcctgtcgt
     2641 ccagtcaaag gagaaaaact ataaagaaat tgtgagaatt tattggactg ctttgttaac
     2701 gtgtcccgtc aggcgcgctt tataacgagc cgccgtgttt ttgtgcaaaa tgcctttatt
     2761 gaccgctttg tcgatggcgg aaatcgtcgg caccagcagt tcctggcttt gttgattgtc
     2821 gctgccggcg atcgccgtgc gaagcttctt aatctgcgtg cgcaggctgc tgcggttgtt
     2881 gcggttgacc agtcggcgtt tttcgttttg tctgacgcgc ttttcggctg atttatgatt
     2941 cggcattaaa aatctccgtt tatatccaaa gtgaaaatat tctcattcag agaaaacatt
     3001 aagtataaat tttagaatta acgatgtcaa ggcgggttta attgtgataa tgtctgagcc
     3061 tctgtactgg tagtttagat taaattcagg tcaatcggcg cgaaataatt ttagaccaga
     3121 taattttcaa agcgaacccg accgcaaaac tacaagccaa cgcaattaga atgtcggcaa
     3181 ttaaaccaga ccaaaagaca taggtgctgt aattgcccga tattccttgt ctatacatca
     3241 aaaaaggaaa gcctctgtca ataatgatat tagaaagaac attggtttca gagggaataa
     3301 atattcaata taaggaatat tataatcccg ctccaaaaac ctacaataaa tgctgtattt
     3361 tgaaatagtt ttttcatagc atcacaaggt tagagtgact aactctaaac gtcaactttg
     3421 gacgcgacgc cgtgcatcgc atcgaccagc agaacgacaa tcggcgggta gaaaataaat
     3481 tccagcctca aggtcggcga aaaaagcttc ggcatcgagg cggcgataaa catttccaga
     3541 taatttccga tgaccatccc gacgacgagg ctgattccta ttccaatcag caaaaacgcc
     3601 accgccgtca gacgcgaata cgggcgcgtt tcctcttcgg tttcttcgag aatcgaatcg
     3661 tgcagtttat tgttgtatgt gtaatggtcg aacccggcgc gcaggcgatt aaccagatga
     3721 atgacgccgg taataaaaag cgtcagcaaa acaatcttgc cgataatttc cgagtttgac
     3781 cagaatttcg gtttgaagat ggcgacgaaa caggtcgaaa gaaaaagcgc gacgattgga
     3841 aaaatcactt tttgcagcgc cagaacttcc ttgcgctcgg cgtcgatgat gctgtcgacg
     3901 attcgattat agcgattttc gaaagctatc tgccgcatcc ggctggcgtg cgagttgtcg
     3961 gaatacataa ccgaatccga atcgtgcagc gagccgttat gaaaaatttt aaaatattgt
     4021 ttatcgtaat gggcgcgttc ctgcgcgttg ctcagaatct cgtaggattt ggctatcaaa
     4081 gcaaattcgc gcgtcgcttc ttccgttccg ccattcacgt ccgggtgcat tttgcgcgca
     4141 agccggcgat aagccgattt tatctcagcc gtggaagcct tcggtgaaat ttttaaaact
     4201 ttgtaatagt ccaccatcgc tttttgaaaa ggagagccgg agagctaagc gcgaagccgc
     4261 tccgccttat gtcaggcaaa ggtgattata acataaatcc gtttcacaaa aaatcttttc
     4321 gcgctcctta acacacgcct ttgcgttatc agctttccat aaaaaagtaa cgcaaaggag
     4381 atggcaaaag tttctgaatt gttgcctcaa aaacagtgaa tttgcctgcg ctcgcctgcg
     4441 aaaaacttcg cgtccttggc gttaagaaaa aaaatcgccg ccgggagcgc aaaattttaa
     4501 gacgaaaaga gaagccgggc tgatttgcag ggcgttataa aaatttaatc caagccgtca
     4561 atctgcaagc cgaccggatt cgcgcccgaa agcttgaccg catcgaccac tttcgccacc
     4621 tcgccgtaag aaaaattttt cggcgctttg ataaaaacgg tttttaaaat tttttcttca
     4681 ttcggcaaat cgtttcgcgc gaccagattt tcgtcataaa ttccgctctg cgagcgctcc
     4741 tcgaatttcg ccgaaagctt ttccgtcaag ctctgcggcg cgccgaccga gccggaatct
     4801 tcctggttga gcttcagcga agaatcggca tttatcgtca cgattaaagt atgcggatgc
     4861 ggcggcaatt tatcagcctt cgtctcctgc ggcactttcg ccttaaaagt cgaaggcttc
     4921 gacggcgaaa taaccataaa aataatcagc agaacaagca aaacgtcgat taaaggcgtg
     4981 acattgatat tcggtttagc ttctgacata agcggtttct ccttttcaaa aagttgtctg
     5041 attagacacc ggaaaagaga aaaagttcca tatgtttaaa taaatttccc ctgaacggcg
     5101 gaaagcagag ccttcgcttt aacaagcgtt tcctcgtatt ctttttcggg aatgctgtca
     5161 atcacgatgc cgccgccgac attgaaaatt gcttcgttgc cgcgcaccac catagtgcgg
     5221 atggcgac
//
LOCUS       DASWNE010000840         2462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_410568, whole genome shotgun sequence.
ACCESSION   DASWNE010000840 DASWNE010000000
VERSION     DASWNE010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410568"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..1454)
                     /locus_tag="VGB68_16450"
     CDS             complement(153..1454)
                     /locus_tag="VGB68_16450"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0050885.1"
                     /translation="MKPIAKVENLSKQYQLGNRAASDTTLRDALGRILRSPLEVFSRR
                     NRKDRKTFWALRDVSFEVEPGEVVGIIGRNGAGKSTLLKILSRVTKPTGGQVRLYGRT
                     NSLLEVGTGFHPELTGRENIFVNGAILGMKRREIERKFDEIVAFAGIDEFLDTAVKHY
                     SSGMYARLAFSVAAHLDSEILIIDEVLSVGDAEFQKKCLGKVTDAARAGRTIFFVSHN
                     MAAIENLCRRGIVLEKGKVTFIGKQTEAISHYLAKADKNLKMDLSRHGDRTGSGEVRV
                     TAIEIRDTRGVHITAAASGQDIDICFHFETAPGYKKKNVMMGFMVRTYWDVPVFLQHN
                     RLTRDEWNALPPKGVFVCRIRRLPLPPSSYRLGFSVMFDDEYLDRIDDASELIVTDGD
                     FYGSGEVPPVSHGCCLVDAEWRLLDSDTAVGLETEKAGLEA"
     gene            complement(1492..>2462)
                     /locus_tag="VGB68_16455"
     CDS             complement(1492..>2462)
                     /locus_tag="VGB68_16455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY0050886.1"
                     /translation="AQIKKFRPRVLLVADYSTFTADFLKNIKRECPFIRLVLGWCGAP
                     YNDASVFGEWDIVLSCIPELIADFRSKGHRCFHVDHAFEPRILDKLDSAQPPSVDFAF
                     VGSILKQSRFHIERERILSELVRHTDLQIWSEIKRPSFKQQRKAFVRRKASEAVAAAN
                     NAGLPRNLINALPLVRRVAQWKPTPIAEQDIDGRIARRTRPPVFGVKMFQLLRDSRVV
                     LNTHIDVSPLSASNMRLFEATGAGACLLTDWKENLARLFEPDKEVLTYSSAAECVEKI
                     RYILAREDERRAIADAGQRRTLREHNFDNRAARIDEIIRQYFAAHK"
BASE COUNT          545 a          710 c          652 g          555 t
ORIGIN      
        1 caaaaaccgt catcccttcg cgcagatact ccagataaaa aatctgttcg gcgcgctcga
       61 cctccgcgta agatgcgggt tcggaggcgg gcgcggcgaa atttcggatt cgccgtttca
      121 gggaattcag aatatttatc atcaatcgca gtttacgctt cgagcccggc tttttcagtt
      181 tctaaaccta ccgccgtatc ggaatccaga agccgccatt ccgcatccac cagacagcag
      241 ccgtgactga ccggcgggac ttcgcccgag ccgtaaaaat ctccgtccgt gacgatcagc
      301 tcgctggcgt cgtcgatgcg gtcgagatat tcgtcgtcga acataacgct gaagcccagg
      361 cgatagctcg acggcggcaa aggcagacgg cgaatgcggc agacaaagac gcctttcggc
      421 ggcagagcgt tccactcgtc gcgcgttaag cggttgtgct gaagaaagac cggcacgtcc
      481 cagtaagttc tgaccataaa acccatcatc acgttctttt ttttgtaacc gggcgcggtt
      541 tcaaagtgaa aacagatgtc gatgtcctga ccggaagcgg cggcggtgat atgaacgccg
      601 cgcgtatctc tgatttcaat cgcggtcacg cgcacctcgc ccgagcccgt gcggtcgccg
      661 tgtcggctca aatccatttt aagatttttg tccgcttttg ccaggtaatg cgaaatagcc
      721 tcggtttgtt tgccgataaa ggtcactttt cctttttcca gaacgattcc gcgacgacac
      781 agattttcga ttgccgccat attgtggctg acgaaaaaaa tcgtccgacc ggctcgcgcc
      841 gcatccgtca ccttgccgag acattttttc tgaaactcgg cgtcgccgac cgacaaaacc
      901 tcgtcgataa tcaagatttc ggaatccaga tgcgcggcga ccgaaaaagc gagccgcgca
      961 tacattccgc tcgaataatg cttgacggcg gtgtcgagaa attcgtcaat tccggcaaaa
     1021 gcgacgattt cgtcaaattt tcgctcaatc tcgcggcgct tcattccaag aatcgcgccg
     1081 ttgacgaaaa tattctcgcg ccccgtcaat tccgggtgaa agcccgtgcc gacttccaga
     1141 aggctgttcg tccgaccgta taaccggacc tgcccgccgg tcggcttcgt cacgcgcgac
     1201 aaaattttga gcagcgtgga tttgcccgcg ccgttgcgcc cgatgatgcc gacgacctcg
     1261 cccggctcga cctcgaaact gacgtcgcgt aaagcccaga aggttttgcg gtcttttcga
     1321 ttccggcgag aaaaaacctc cagcggcgag cgaagaattc tgccgagcgc gtctcttaaa
     1381 gtggtatcgg acgcggcacg atttccgagc tgatactgtt tacttaagtt ttcgaccttc
     1441 gctatcggtt tcattggatt ccggtttatc ggctttgtcc gggcgcattc tctacttgtg
     1501 agccgcaaaa tattgcctga tgatttcgtc aattcgcgcc gcccggttat cgaaattatg
     1561 ttcgcgcagc gtgcggcgct gtccggcgtc ggcgatggcg cgtcgttcgt cttcgcgcgc
     1621 gagaatgtat cggatttttt cgacgcattc cgccgccgag ctgtaagtca gcacctcttt
     1681 atccggctcg aataaccgcg ccagattttc cttccaatcc gtcagcaggc aagcgcccgc
     1741 gccggtcgcc tcgaaaaggc gcatattgga cgcgctgagc ggcgatacgt cgatgtgcgt
     1801 attaagaacg acgcggctgt cgcgaagcag ctgaaacatt tttacgccga aaaccggcgg
     1861 tcgcgtccgg cgcgcaatgc gcccgtcaat atcctgctcc gcaatcggcg tcggcttcca
     1921 ctgcgcgact ctgcgcacca acggcagcgc atttatcaaa ttccgcggca agcccgcatt
     1981 atttgccgcc gcgaccgctt ccgaggcttt gcggcgaaca aaggcttttc gctgctgctt
     2041 aaaggacggg cgttttattt ccgaccagat ttgcaaatcc gtgtgcctga ccagctcgga
     2101 taaaatccgc tcgcgctcga tgtgaaaacg gctttgcttt aaaatcgagc cgacaaaggc
     2161 aaaatcgacg gacggcggct gagccgaatc gagcttgtcc aaaatgcgcg gctcgaaagc
     2221 gtgatcgacg tgaaagcaac ggtgtccttt acttcggaaa tcggcgatta attccggaat
     2281 gcacgacagc actatatccc attcgccgaa aaccgacgcg tcattatagg gcgcgccgca
     2341 ccagccgagc accagccgga taaaaggaca ttcgcgtttt atgtttttga gaaaatcggc
     2401 ggtgaaagtg gaataatccg cgaccagaag cacacgggga cgaaactttt taatctgcgc
     2461 gg
//
LOCUS       DASWNE010000841         5075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_167855, whole genome shotgun sequence.
ACCESSION   DASWNE010000841 DASWNE010000000
VERSION     DASWNE010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167855"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1558
                     /locus_tag="VGB68_16460"
     CDS             <1..1558
                     /locus_tag="VGB68_16460"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HEY0050887.1"
                     /translation="TFIKGDRMTAELYANKKVRSTLVYGNAYLKQTAPDRATEVSANE
                     LNANFAENQQVQNAKASGSANVTLVPAQPQDFTKATMSAPVAVRLTFANNVLNQMQTE
                     GRTTIALTAPQGKADASNKKLIADTVKTDLNAGGRDILKAEAVGNAELYVEPLQANAE
                     NYKTTITAPRFDCDFYAGNNPKTCVADKKAKAVQQPTIAAENRGTRTLTADKLTSSFN
                     QNTHDVERFDAVGNAKFSELDRNGIASQIIFTANDGTVRLRGGEPTVWDSVGRAKATE
                     IDWDTRGEKSLLRGRVSTTYYSQKQMGGTTPFGKTSAPVFVTAERADFDHRGEVAAYT
                     GNARAWQENNYVRAEKLVFQQKTKRMDGEGKVQSLLYNAKRKENGKEFTQPVFASSER
                     ISYTDQNRQLRYEGNVDIRQGTDRIVSGITDVFLNENNEVTQTIAQNNVVVTQPNRRA
                     AGDYAQYTTADEVVILRGNPASVEDAEQGSSQSAQLTVNLRDNRIVSQGSTKQTGTGR
                     IRSVYKVKKQ"
     gene            1725..2510
                     /gene="lptB"
                     /locus_tag="VGB68_16465"
     CDS             1725..2510
                     /gene="lptB"
                     /locus_tag="VGB68_16465"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012322358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEY0050888.1"
                     /translation="MFKVFRSNNSQHSNGSYEEYPTNALAAVHLQKTYGRRRVVDDVS
                     LFVERGEVVGLLGANGAGKTTTFYMMTGLETAEHGNIFLSGQNVTTLPMYLRARLGLG
                     YLPQEPSIFRKMSAEQNILAVLETMKISRADRFARLEELLEEFGVVHVRKTRGDALSG
                     GERRRVEIARCLATEPQFILLDEPFAGIDPIAIDEIREIILYLKSRGIGILITDHNVR
                     ETLGITDRAYIMAEGKILRTGKPEDLVKDPEVRRLYLGERFSL"
     gene            2707..4278
                     /gene="rpoN"
                     /locus_tag="VGB68_16470"
     CDS             2707..4278
                     /gene="rpoN"
                     /locus_tag="VGB68_16470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEY0050889.1"
                     /translation="MSSLKLTQSLKQQMVLTPQLRQRIEMLQMTSLELTDLIQQEMIA
                     NPILEEVTNDEEVQEISEKILDQNSDGYEDPHENGYDEGTAEFAQKALSEFEDFTPEA
                     VSENGFKEEHGGDVENDDILPHESGDAFEEVDFGREFQDYLDPGYKTQEIEYKEDAPS
                     FEQFLTRSESLTEHLEWQLNLQQISEQLEEAGICIIGNIDGDGRLLASNEEIGEMCGC
                     TPEQVEKARQIVLKLEPVGCGARTVQECLAAQLEAIGEGESLAAELVKNHLHDLQPHR
                     LQHLSKQTGVELHELNEEINKIRGLDPYPGRRYSSEEPIFVSPEVYIEKLEDDYVIYF
                     TDDGSPRLRISSTYQSLLEKNNTTKETKDFIKEKVRSAVDLLRNIEHRRQTIYRVVEC
                     IVQRQREFLDKGVEHIKPMMLKDVAEDIGMHLSTVSRVVNRKYAHTPQGVIELRRFFS
                     EGMLNEDGEEVSTRIFKLRIKKMIEDEDTKKPLTDDEIAKILSKEGVKLSRRTVAKYR
                     DQMQIPGSRERKTVV"
     gene            4409..4939
                     /gene="raiA"
                     /locus_tag="VGB68_16475"
     CDS             4409..4939
                     /gene="raiA"
                     /locus_tag="VGB68_16475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEY0050890.1"
                     /translation="MKFEYTGRHLEVTPALRSHVENHFDKLKHLFNGQATNAHVIIEV
                     AKGRHRAEIIVNWRDHVLTANTTVSDMYKALTQTITKLEKQATRLKDKVIDQHHRATK
                     TSIVATPVDEIPPAPGAPTIINSSSYMVKPMTPEEAILNLQTEENQFLVFRDAENEKV
                     SVIYRRADGNFGLIQP"
BASE COUNT         1451 a         1270 c         1328 g         1026 t
ORIGIN      
        1 aacttttatt aaaggcgacc ggatgacggc ggagctttac gcaaataaaa aagtccgcag
       61 cactctggtt tacggcaacg cttacctgaa acaaacagcg cccgaccgcg cgaccgaagt
      121 ttcggcgaac gagctgaacg ccaatttcgc ggaaaatcaa caggttcaaa atgcgaaagc
      181 gagcggttcg gcaaatgtta cgctcgttcc cgcgcagccg caggatttca cgaaagcgac
      241 gatgtccgcg ccggtcgcgg tgcgtctgac ttttgcgaat aacgttttga atcagatgca
      301 gacggaaggt cgcacgacca tcgcgctgac cgcgccgcag ggaaaagccg acgcttccaa
      361 taaaaagctg atcgccgaca cggtgaaaac cgacttgaat gcgggcggca gagatatttt
      421 gaaagccgaa gccgtcggca acgcggaatt gtacgtcgag cctttgcagg cgaatgcgga
      481 aaactacaaa acgaccatca ccgcgccgcg tttcgattgc gatttttatg cgggaaacaa
      541 tccgaaaacc tgcgtcgccg acaaaaaagc gaaagccgtc caacagccga caatcgccgc
      601 cgaaaatcgc ggaacccgca cgctgacggc ggacaagctc acttcgagtt ttaatcagaa
      661 cacgcacgac gtcgaacgat tcgacgccgt cggcaacgcg aaattttcgg agctcgaccg
      721 caacggcatc gccagccaga taatttttac ggcgaatgac ggaaccgtgc gcctgcgcgg
      781 cggcgagccg accgtctggg attcggtcgg gcgcgccaaa gcgacggaaa tagactggga
      841 cacgcgcggc gaaaaatcgc ttttgcgcgg tcgcgtttcg accacctatt acagccagaa
      901 acagatgggc ggcacgacgc ctttcggcaa aacgagcgcg ccggttttcg tcacggcgga
      961 acgcgccgat ttcgaccatc gcggcgaggt cgccgcttac acgggcaatg cccgcgcgtg
     1021 gcaggaaaat aattacgtgc gcgccgaaaa actggttttt cagcagaaaa caaaacgaat
     1081 ggacggcgaa ggaaaagttc aaagcctgct ttacaacgcg aaacgaaagg aaaacggcaa
     1141 ggaattcacg cagccggttt tcgcttcgtc cgagcgaatt tcctataccg accagaacag
     1201 gcagctgcgc tacgaaggaa acgtcgacat ccggcaggga accgaccgca tcgtctccgg
     1261 cataacggac gtttttctta acgaaaataa cgaagtgacg cagacgattg cgcagaacaa
     1321 cgtcgtcgtc acgcagccga accggcgcgc cgccggcgat tacgcgcaat acacgaccgc
     1381 cgacgaagtc gttatcctgc gcggcaatcc ggcgagcgtc gaagacgccg agcagggctc
     1441 atcgcaaagc gcgcaattga cggttaatct gcgcgataac cgcatcgtca gccagggctc
     1501 gaccaaacaa accggaaccg gcagaatccg ctcggtttat aaagtaaaaa aacagtagcg
     1561 gaataagacg aaagggaaaa agtaaaaaag caaaagcgtg gaattggttt ctcacttttg
     1621 ctttttactt tttccctttc gtctttcttt ttttcttatc tcaaaattac attaaaataa
     1681 acggcgaatg aaaaaaagga aaaattaagc taaactttct ctgaatgttt aaagttttcc
     1741 gttccaacaa ttcgcagcac tcaaacggca gctatgaaga atatccgaca aacgcgctgg
     1801 ctgccgtgca tttgcagaaa acttacgggc ggcggcgcgt ggtcgacgat gtcagtcttt
     1861 ttgtcgagcg cggcgaggtg gtcgggcttc tgggcgcgaa cggcgccgga aaaacgacga
     1921 cgttttatat gatgaccggt ttggagacag ccgagcacgg caatattttt ctgagcggtc
     1981 agaacgtaac gacgcttccg atgtatctgc gcgcgcggct cggcttgggt tatctgccgc
     2041 aggagccttc gattttccgc aaaatgagtg ccgagcagaa cattctcgcc gtgctggaaa
     2101 cgatgaaaat ctcgcgcgcc gaccgttttg cccgtttgga agaattgctg gaagaattcg
     2161 gcgtcgtgca cgttcgcaaa acgcgcggcg acgcgctttc cggcggcgag cgtcgccgcg
     2221 tcgagattgc gcgctgtctg gcgaccgagc cgcagtttat tcttctggac gagccttttg
     2281 ccggcatcga cccgatcgcc atcgacgaaa tccgcgagat tattctttat ctgaaaagcc
     2341 ggggcatcgg cattttaatc accgaccaca acgtccgcga aacgctcggc atcaccgacc
     2401 gcgcctacat tatggcggaa ggcaaaatcc tgcgcacggg caagcccgaa gatttggtga
     2461 aagacccgga agtccgaagg ctttatctgg gcgaaagatt cagcctgtag ggaattacga
     2521 attacaaatt acgaattacg aattacgaat gaaggaaatt ttccgaaaaa catctcgaat
     2581 ctttcttcca attcgtaatt cgtaattcgt aatttatttt cacctctctt actttttccg
     2641 tgcaaaatcg gtataatctt ctcaaggaat atacaaagga taacgagcca aaagtcttag
     2701 caaaatatgt cgtcattaaa actgactcaa agtctgaaac aacaaatggt tttgactccg
     2761 cagcttcgcc agcggataga aatgttgcag atgaccagtc tcgaactcac cgatttgatt
     2821 cagcaggaaa tgattgccaa tccgattctg gaagaagtga ccaacgacga ggaagtgcag
     2881 gaaatttccg aaaaaattct tgaccagaac tctgacggct acgaagaccc gcacgaaaac
     2941 ggttatgacg aaggaacggc tgaatttgcg cagaaagcgc tgagcgaatt tgaagatttt
     3001 acgcccgaag ccgtcagcga aaacggtttt aaggaagaac acggcggcga tgtcgaaaac
     3061 gacgacattc tgccgcacga atcgggcgac gcttttgaag aagtcgattt cgggcgcgag
     3121 tttcaggatt atctcgaccc gggatacaaa acacaggaaa tcgaatataa agaagatgcg
     3181 ccgagtttcg agcagtttct gacgcgctcg gaatcgctga ccgagcatct ggaatggcag
     3241 ctgaatctcc agcaaatcag cgaacaactg gaagaagccg gaatctgcat catcggaaat
     3301 atcgacggcg acgggcgctt gctcgccagc aatgaagaaa tcggcgagat gtgcggctgc
     3361 acaccggaac aggtcgagaa agccagacaa atcgtcttaa agctcgagcc ggtcggctgc
     3421 ggcgcgcgaa ccgttcagga atgtctggcg gcgcagctcg aagcgattgg cgagggcgaa
     3481 agcctggcgg ctgagctggt caaaaatcac ctgcacgatt tgcagccgca tcgccttcag
     3541 catctgtcga aacaaaccgg cgtcgagctg cacgaactga acgaggaaat caataaaatt
     3601 cgcggtctcg acccgtatcc cggcagacgt tattcgtcgg aagaaccgat tttcgtctcg
     3661 cccgaagttt atatcgaaaa gctggaagat gattacgtta tttattttac cgacgacggc
     3721 agcccgcgcc tgcgcatcag ctcgacttac cagtcgcttt tggaaaaaaa caacacgacc
     3781 aaggaaacca aggatttcat caaggaaaaa gtgcgctcgg cggtcgattt attaagaaat
     3841 atcgagcacc ggcggcagac gatttatcgc gtcgtcgagt gcatcgtcca gcgccagcgc
     3901 gagtttctgg acaagggcgt cgagcatatc aagccgatga tgctgaaaga tgtggcggaa
     3961 gatatcggaa tgcatctttc gaccgtctcg cgcgtcgtca atcgcaaata cgcgcacacg
     4021 ccgcagggcg tcatcgagct gcgccggttt ttctcggaag gaatgctcaa cgaggacggc
     4081 gaggaagttt cgacgcgcat ttttaaattg agaatcaaga aaatgattga agatgaagat
     4141 acgaaaaagc ccctgaccga tgacgaaatc gccaaaatcc tcagcaagga aggcgtcaag
     4201 ctgtcgcgtc ggacggtggc gaaataccgc gaccagatgc agattcccgg ctcgcgcgaa
     4261 agaaaaactg tagtttgata aatcaaacgg tcttcggttt tcggtcttcg gtcttcgtta
     4321 aaaaattatg ctcgacattg agcggtaaaa aatcgaagac cgaagacccg aagtccgaag
     4381 accgtaaaaa gaacaagagg tgcgatttat gaaatttgaa tataccggaa gacacttaga
     4441 ggtaactcct gcattgcgtt ctcacgtcga gaaccatttt gacaaactga aacatctttt
     4501 taacggtcag gcgacaaacg cccacgttat tattgaagtc gcgaagggaa gacatcgcgc
     4561 cgaaattatc gtcaactggc gcgaccacgt gctgacggcg aacacgacgg tttcggatat
     4621 gtataaagcc ttgacgcaga cgataacgaa actggaaaaa caggcgacaa ggttaaaaga
     4681 taaggtcatt gaccagcatc atcgggctac gaaaaccagc attgtcgcga cgccggtcga
     4741 cgaaattccg cccgcgccgg gcgcgccgac cattatcaat tccagcagtt atatggttaa
     4801 gccgatgacg ccggaagaag caattttgaa tctgcaaacg gaagaaaatc agtttcttgt
     4861 tttcagagat gccgaaaatg aaaaagtttc ggttatctac cgtcgtgcgg acgggaactt
     4921 cgggctaata caaccgtaag gaattttaga ttttcgattt tcgattttag attggataag
     4981 gaaaataaat tttaaaatcc ttatccaatc gcgaatcgca aatccaaaat cgaaaatcag
     5041 gaatgtcttc aaacttcaaa aacgaaaaac cgagc
//
LOCUS       DASWNE010000842         4879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_339822, whole genome shotgun sequence.
ACCESSION   DASWNE010000842 DASWNE010000000
VERSION     DASWNE010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339822"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..4134
                     /locus_tag="VGB68_16480"
     CDS             13..4134
                     /locus_tag="VGB68_16480"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0050891.1"
                     /translation="MASNLTGQVRNIAAVTTAVANGDLSKKITVDVQGEILELKDTIN
                     TMVDQLSSFASEVTRVAREVGTEGKLGGQAVVRGVSGTWKDLTDNVNFMASNLTSQVR
                     NIADVTTAVATGDLSKKITVDVKGEILELKNTINTMVDQLSSFASEVTRVAREVGTEG
                     KLGGQAVVRGVSGTWKDLPDNVNFMASNLTSQVRNIATVTTAVANGDLSKKITVDVQG
                     EILELKITINTMVDQLNSFASEVTRVAREVGTEGKLGGQAEVKGVAGVWKDLTDNVNF
                     MASNLTTQVRGIARVVTAVANGNLNRKLEVEALGEIAELADTINAMIDTLATFADQVT
                     TVAREVGIEGELGGQANVPGASGTWRDLTDNVNQLAANLTNQVRAMADVATAVTKGDL
                     TRSITVDASGEVAFLKDNVNEMIRNLRETTQKNTEQDWLKTNLAKFTRLLQGQRDLLT
                     VARLILSEMAPLVEMQHGVFYITETVEDETILKLLASYAYRERKSVANEFRLGESLVG
                     QCALEKERIIITNVPPDYVKINSGLGEAVPKNIVVLPVLFEGQVKAVIELASFEAFNE
                     IHFGFLDQLTESIGIVINTIEANMRTEELLKQSQSLAQELQVGQAELQETNRRLELQA
                     QSLQTSEELLKQQQEELQQTNQELEEKAQLLLEQKSEVEQKNREIELARVALEDKAKQ
                     LALSSKYKSEFLANMSHELRTPLNSMLILSKMLSENPDGTLNEKQIEFAETIHSSGAD
                     LLALINEILDLSKIESGTMGVEIGEVHFGELQGDIERTFNQIAQDKGLQLVIELDEKL
                     PRHMQTDVKRLRQVLKNLLSNAFKFTHQGQVAVQVCTAESGWSYDNDNLNRASSVIAF
                     KVSDTGIGIPEDKQQLIFEAFQQADGTTSRKYGGTGLGLSISREIARLLGGEITLESR
                     PGEGSQFTFYLPENYQPTVLTPQDDEEEFSAANGHARPSLISLSRESEDEAISRVQII
                     VSDDRAEIKPDDRVLLIVEDDSTFARILVDIAHEQGFKCLVAQRGEDALMTARKYRPD
                     AVTLDLTLPGMHGYAVLDRLKHDPSTRHIPVQIISVIDDLRRVLKLGALGHITKPVNR
                     EKLVEGVVSLKDFVERPLKKLLLVEDDETLQRSINELIGGEDLEITSVGTGEEALNAL
                     QQTRFDCAIVDLMLPDVEWSELIGKIKQKSGFPELPVIVHTGKDLTKRETSRIKKLAE
                     TIIIKDADSLERLVDETALFLHRDVADLSASQKNMMMRVNRVDPRLEGKKVLIVDDDI
                     RNIFALTSALERQQMQVVYAENGRDGIETLKKTPGIEGVLMDIMMPEMDGYEAMHEIR
                     QIPKFKSIPIIAVTAKAMKGDRQKCLEAGASDYITKPVDTEQLLSLLRIWLDK"
     gene            4209..>4879
                     /locus_tag="VGB68_16485"
     CDS             4209..>4879
                     /locus_tag="VGB68_16485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0050892.1"
                     /translation="MASEYQANVLLVDDRPENLLAMETILADLGQNLIRASSGREALR
                     FLLVEDVALILLDVQMPGLNGFEMAELIRERERTQRTPIIFVSATSKDEQYVFKGYSL
                     GAVDYLTKPFEPEILKSKVRFFTELFRQNQEIKRQAALLEEANAELDDLNSELEERVR
                     RRTTQLEAANRELENEIAVRKQSEARLATEHSITRTLADARSLEAAAPVILKTFCDHL
                     QASIS"
BASE COUNT         1244 a         1302 c         1379 g          954 t
ORIGIN      
        1 aacgtaaact ttatggcgtc gaacctgacc ggacaggtgc gcaacatcgc ggcggtaaca
       61 accgcagtgg caaacggcga cctttccaaa aaaatcaccg tcgacgtgca gggagaaatt
      121 ttagagctca aagacaccat caacacgatg gtcgaccagc tttcgtcttt tgcatcggaa
      181 gtgacgcgcg tggctcgtga agtgggaacc gaaggaaagc tcggcggtca agccgtcgtg
      241 cgcggcgttt cgggaacgtg gaaggatttg accgacaacg taaactttat ggcgtcgaac
      301 ctgacttcgc aagtgcgcaa catcgccgac gtgacgacgg ctgtggcgac gggcgacctt
      361 tccaaaaaaa ttacggtgga cgtaaaaggc gagattctgg aattgaaaaa caccattaat
      421 acaatggtcg accagctttc gtcattcgcc tcggaagtga cgcgcgtggc gcgtgaagtg
      481 ggaaccgaag gaaagctcgg cggtcaagcc gtcgtgcgcg gcgtgtcggg aacgtggaag
      541 gatttgcccg acaacgtaaa ctttatggcg tcgaacctga cttctcaggt gcgcaacatc
      601 gcaactgtga cgaccgccgt ggcgaacggc gatttgtcga aaaaaatcac ggtcgacgtg
      661 cagggcgaaa ttcttgagct gaaaatcacg attaacacga tggtcgacca gctcaattca
      721 ttcgcatcgg aagtgacgcg cgtggctcgc gaagtgggaa ccgaaggaaa gctcggcggt
      781 caggcggaag taaaaggcgt cgcaggcgtc tggaaagact tgaccgacaa cgtgaacttt
      841 atggcgtcga acctgacgac gcaggtgcgc ggtatcgcgc gcgtcgtgac cgccgtggca
      901 aacggcaacc tgaatcgcaa gctcgaagtg gaagcgctcg gcgaaatcgc cgagcttgcc
      961 gacaccatca acgcgatgat cgacacgctc gccaccttcg ccgaccaggt gacgaccgtg
     1021 gcgcgcgagg tgggaatcga aggcgaattg ggcggacagg caaacgtgcc gggcgcttcg
     1081 ggaacgtggc gcgatttgac cgataacgtg aaccagctcg ccgccaacct gaccaaccag
     1141 gtgcgcgcga tggctgacgt ggcaaccgcc gtgaccaagg gcgacctgac gcgctcgatt
     1201 acggtggacg cgtccggcga agtcgcgttt ttgaaagata acgtaaacga gatgattcgc
     1261 aacctgcgcg aaacgacgca gaaaaacacc gagcaggact ggctgaaaac caacctcgcc
     1321 aaatttacgc gcctcttgca gggtcagcgc gatttgctga cggtggcgcg gcttattctt
     1381 tcggaaatgg ctccgctcgt cgagatgcag cacggcgttt tctacattac cgagacggtc
     1441 gaggacgaaa cgattctcaa gctcctcgcc tcttacgctt accgcgaacg taaatccgtc
     1501 gccaacgagt ttcgcctggg cgaaagtctg gtcgggcaat gcgcgctgga aaaagaaagg
     1561 attatcatca cgaacgtgcc gcccgattac gttaaaatca attccggctt gggcgaagcc
     1621 gttccgaaaa acatcgtcgt cttgcccgtt ctgttcgagg gacaggtcaa agccgttatc
     1681 gagcttgcat cttttgaagc gtttaacgaa attcacttcg gttttctcga ccagttgacc
     1741 gaatcaatcg gtatcgttat caacacgatt gaagcgaata tgagaaccga agagctgctc
     1801 aaacaatcgc agtcgctcgc gcaggaattg caggtcggtc aggcggaatt gcaggaaacg
     1861 aaccgccgct tggaacttca ggcgcagtcg ctgcaaactt ccgaagagct gctcaaacag
     1921 cagcaggaag agctccagca gaccaaccag gagctcgaag aaaaagcgca gcttctgctc
     1981 gaacaaaaga gcgaggtcga acagaaaaac cgcgaaatcg agctggcgcg cgtcgccctc
     2041 gaagacaaag cgaaacagct cgctctctcg tcgaaataca aatccgagtt tctggcgaat
     2101 atgtcgcacg aattgcgcac gccgctcaat tcgatgttga ttctatctaa aatgctttcg
     2161 gaaaacccgg acggcacgct caacgaaaaa caaatcgagt ttgccgaaac gattcactcg
     2221 tcgggcgcag accttctggc gctgattaac gaaattctcg atttatcgaa aatcgaatcc
     2281 ggcacgatgg gcgtcgaaat cggcgaagtc cacttcggcg aattgcaggg cgacatcgag
     2341 cggacattta accagattgc gcaggacaaa ggcttgcagc tggtcatcga gctggacgaa
     2401 aaattgccca gacatatgca aaccgacgtc aaacgcctgc gtcaggtgct caaaaatctg
     2461 ctctcgaatg cctttaaatt tacgcatcag ggacaagtcg ccgtgcaggt ctgcacagcc
     2521 gaaagcggct ggagctatga taacgacaat ctgaatcgcg cttcctcggt tatcgctttt
     2581 aaagtcagcg acaccggcat cggcattccc gaagacaagc agcagcttat tttcgaggcg
     2641 tttcagcagg cggacggcac gaccagccgc aaatacggcg gaacgggtct ggggctttcc
     2701 atcagccgcg agattgcgcg tcttctgggc ggcgaaatca ccctggaaag ccgaccgggc
     2761 gaaggcagcc agtttacttt ctacctgccg gaaaattacc agccgaccgt tttaacgccg
     2821 caggatgatg aagaagaatt ctcggcagcc aacggtcacg cgcgaccgag cctgatttcc
     2881 ctctcgcgcg aatccgaaga cgaagcgatt tcgcgagtgc aaatcatcgt cagcgacgac
     2941 agagccgaga ttaagccgga cgaccgcgtg ctgctgattg tcgaagacga cagcactttc
     3001 gcgcgcattc tggtcgatat cgcccacgag cagggcttta aatgcctggt cgcgcagcgc
     3061 ggcgaagacg ccctgatgac ggcgcgaaaa tatcgtcccg atgccgttac gctcgattta
     3121 accttgccgg gaatgcacgg ctacgccgtt ctcgaccgcc tgaaacacga cccttcgacg
     3181 cgtcatatcc cggtgcagat tatttcggtc atcgacgatt tgcggcgagt tctgaagctc
     3241 ggcgcgctcg gacacatcac caagccggtc aatcgtgaaa aactggtcga aggcgtcgtc
     3301 agcctgaagg actttgtcga gcgtccgttg aaaaaacttc ttctcgtcga agacgacgaa
     3361 acgcttcagc gcagtatcaa cgaattaatc ggcggcgaag acttagagat aacgtcggtc
     3421 gggacgggcg aggaagcttt gaacgctttg cagcagacgc gttttgactg cgcgattgtc
     3481 gatttgatgc tgcccgatgt ggaatggtct gagctgatcg gcaaaatcaa acagaaatcg
     3541 ggctttccgg agcttccggt catcgtccac acgggcaagg atttgacgaa gcgcgaaact
     3601 tccagaatca aaaagctcgc cgaaacgatt atcatcaaag acgccgattc gctggaaaga
     3661 ttagtcgacg aaaccgcgct gtttctgcat cgcgacgtcg ccgatttaag cgcctcgcaa
     3721 aagaatatga tgatgcgcgt caatcgcgtc gacccgcggc tggaaggcaa aaaagtgctt
     3781 atcgtcgacg acgatattcg caatatcttc gctttgacca gcgcgctcga acgccagcag
     3841 atgcaggtcg tctacgccga aaacgggcgc gacggcatcg aaacgctcaa gaaaacgccc
     3901 ggcatcgaag gcgttctgat ggacattatg atgccggaaa tggacggtta cgaggcgatg
     3961 cacgagattc gccagattcc gaagttcaag tcgattccga ttattgccgt gaccgccaaa
     4021 gcgatgaagg gcgaccgcca gaaatgtctg gaagccggag cgtcggatta tattactaag
     4081 cccgtcgata ccgaacaact gctttcgctc ttgcgtattt ggctcgataa gtagaaaaat
     4141 agaagagtgg aaagcaaatg tcccgaaact gaggcatttg ctttccgctt tgagattaag
     4201 gagggttaat ggcttcggaa tatcaggcaa acgttctgct ggttgacgac cgaccggaaa
     4261 atctgctggc gatggagacg attctggcgg atttgggtca gaatttaatt cgcgcctcgt
     4321 ccggtcgcga agccctgcgt tttctgctcg tcgaagatgt cgccttgatt ctgcttgacg
     4381 ttcagatgcc gggtttgaac ggttttgaaa tggcggaatt gattcgcgag cgcgagcgga
     4441 cgcaacggac gccgattatt tttgtctccg ccaccagcaa ggacgagcag tacgtcttta
     4501 aaggctactc gctcggcgcg gtcgattatc tgaccaagcc gttcgagccg gaaattctca
     4561 aatcgaaagt tcgttttttc accgagcttt tccgccagaa tcaggaaatt aaacgacaag
     4621 ccgctctgct cgaagaagcc aacgccgagc tggacgattt aaactcggaa ttggaagagc
     4681 gcgtccgccg gcgcacgacg cagctcgaag ccgccaaccg cgagctggaa aacgagatag
     4741 ccgtccgcaa acaatccgaa gcgcggctgg cgaccgagca ttccattacg cgaacgctcg
     4801 ccgatgcgcg cagtctggaa gccgccgcgc cggtgattct aaaaactttc tgcgaccacc
     4861 tgcaggcgag catcagctg
//
LOCUS       DASWNE010000843         1967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_98720, whole genome shotgun sequence.
ACCESSION   DASWNE010000843 DASWNE010000000
VERSION     DASWNE010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98720"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            599..1924
                     /locus_tag="VGB68_16490"
     CDS             599..1924
                     /locus_tag="VGB68_16490"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEY0050893.1"
                     /translation="MELVAFEVLLVFVLLIVNGIFSMSELSVVSARRIRLRQQAENGS
                     KGAQTALDLAESPNKFLSTVQIGITLVGILSGAFGGATIAGHLGDLLNGFAFIAPYGE
                     AVSFALVVAVITYFSIIIGELIPKSIALNSPEKVASMVSRPMKFLSRLSSPLVWLLTA
                     PTAFVLKLFKVHASVEPPVTDEEIKGLIDAGTKAGVFEETEQDLIESIIHLGDRAIVS
                     VMTPRTKIAWLNTDDSPEKIKEELINSGYSRLPVGEGSLDAVIGYASAKSLLSYFLKE
                     SEIDLKATLKQPLYVPETLTALELLEQFKEANTLFAMVVDEFGGVEGLVTMHDVLEAI
                     IGDLPTSQRTASKQDMTLLENGSWLASGQVPTIDFKETLGIKELPFEERDQYHTLAGF
                     AISQLGKVPEAGENFEWNNFRFEIVAMDRNRVDKILVSPLSSAAEDTSD"
BASE COUNT          508 a          435 c          483 g          541 t
ORIGIN      
        1 cgaaaattaa gtaaacaaaa aatgttgtca agataaattc cgtcctgatt tgcgcttgct
       61 catttcgctt ttttcctgag acataaataa taatccgcgc cgcaatccaa aaaggcagtg
      121 ttaccgcaat caggaaaatt attgatttga ataaatgatt catcaggctt tctgattcgt
      181 ccggctacaa ttagacgcaa gttggacgag tttttatacc gctaaacagc cgcatacaac
      241 tcaaatacgt gaaaataccg caaatccgtc tcaaaaaggg gcgtttacgg gacagctttc
      301 gattttttga aatcgaaagc tgattctgct atttacttaa taagcaacag gttggttttc
      361 gataaaatgc gtttataaaa ttttctcatt gttcccgctt ttatttaata aaaacgcttg
      421 tcgtgcaaaa tcgtcgcgaa aaaggtcgcc ggtgaacttt ttcaaaagga ctatattata
      481 gattttattt ctatgaaact ataacaaatc tttcaaaacg ggaatccggt gtaaaatatc
      541 tttaattaat gcaaagctgt gattattaca gcgcatttgt gcgttcaccg gagtttatat
      601 ggaattagtg gcttttgagg ttttgctggt ttttgtgttg ttgatcgtta atggaatatt
      661 ctcaatgtcg gaactgtcgg tcgtctcggc gcggcggatt cgcctccggc agcaagccga
      721 aaacggaagt aaaggcgctc aaactgcttt agatttggct gaatcgccga ataagtttct
      781 ttctaccgtc cagattggta ttactctggt gggtattcta tccggcgctt tcggaggagc
      841 gaccattgcc ggacatttag gcgacctgct taacggattc gcctttatag ctccgtacgg
      901 cgaagctgtc agttttgctt tagtggtcgc cgtcattact tatttttcca ttatcatcgg
      961 cgagctgatt ccgaaaagca ttgctcttaa ttcgcccgaa aaagttgctt caatggtttc
     1021 gcgcccgatg aaatttctgt cgcggctttc ttcgccttta gtgtggctcc tgaccgcgcc
     1081 gaccgctttc gttttaaagc ttttcaaagt tcacgcatcg gtcgagccgc ccgtgacgga
     1141 cgaagaaata aaaggcttga tagacgccgg cacgaaagcc ggagtttttg aggaaacgga
     1201 acaggatttg attgaaagca ttattcattt aggcgaccgc gcaatcgtct cggtcatgac
     1261 gccgcgcacg aaaatcgcct ggctcaacac ggatgattct cctgaaaaaa ttaaggaaga
     1321 attgataaac agcggttatt cgcgacttcc tgtcggcgaa ggcagtctgg acgccgtcat
     1381 cggttatgct tcagccaaat ctttgctgtc gtatttcctg aaagagtcgg aaattgattt
     1441 gaaggctact ttaaaacaac ctttatatgt gcccgagacg ctcacggctc tggaattatt
     1501 ggagcaattt aaggaagcca atactctttt cgccatggtc gtcgacgagt tcggcggcgt
     1561 cgaggggctg gtgacgatgc acgacgtgct ggaagcgatt atcggcgatt tgccgacgag
     1621 ccagcgaacc gcgtcaaaac aggatatgac gctgctggaa aacggctcct ggctggcgag
     1681 cgggcaggtt ccgacgattg acttcaagga gaccctcggt atcaaagagc tgccgtttga
     1741 ggagcgcgac cagtatcaca cgctcgccgg gttcgccata tcgcagctgg gcaaagtgcc
     1801 ggaagcggga gaaaatttcg agtggaataa cttccggttc gagatcgtgg caatggacag
     1861 aaaccgcgtg gacaaaatac tggtttcgcc gctttcgtcg gcggcggaag atacgagcga
     1921 ttaagagtcg ttattcctga gccgttccgg cagacgcctg cacctct
//
LOCUS       DASWNE010000844         2656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_379722, whole genome shotgun sequence.
ACCESSION   DASWNE010000844 DASWNE010000000
VERSION     DASWNE010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379722"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..1087)
                     /locus_tag="VGB68_16495"
     CDS             complement(140..1087)
                     /locus_tag="VGB68_16495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY0050894.1"
                     /translation="MLAVLGALVANGLITILKFIGALLTGSSGMMAESLHSLADTTNQ
                     VFLLLGLRFYKRPASEKHPFGYGKERFFWSFIAAIFIFGVGATYAIYEGIEKVRHPHE
                     PENLFWAYWILGISFVLEAGSIALALYQEINEAHHEGMTFGEYLRESKDPTAKTVIFE
                     DSAALLGIVIAALGLLLGQYHVPPYEGRLWDGIASITIGVVLAIVAFGLARSSRGLLL
                     GEAATAKNVEKIRQAVKTHPNVVEVVELLTMHLAPKQILVNAHVNLKDDLTNNEVIQT
                     IAEIEEAMKKAEPKVDMIFLETARQKESSVPALLPQHIG"
     gene            complement(1251..2354)
                     /locus_tag="VGB68_16500"
     CDS             complement(1251..2354)
                     /locus_tag="VGB68_16500"
                     /inference="COORDINATES: protein motif:HMM:NF024790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY0050895.1"
                     /translation="MNVLEFSKRQLLVAHVFDIPVRVDYRWFGVLILISVLTAVNIPV
                     ELIENFGARLVLAFISTLIFFASILFHELAHAFTARREGVEVLEIVLHPFGGLARFRR
                     EPDTPRAEFRIAIAGPFASFLLALVFLLLTALSLFLQTDVLTPLLRMLFFGNMLLAIF
                     NLFPGYPLDGGRVLRAFLWRRGTDLNEATITTGRAGQIIAIALISVGLYISFVRGDFF
                     TGIWTVLVGLFLLDAATGIIKQASKNENFLVEEAMTLPVAVEPDASVLHFIDHILPVH
                     RQSVFLVAKDRQLYGILTLADLKKIPREDWNKKQVLEVMRPVTTDYFVENNSPIADAR
                     ELMRANGIGALGVIDENGELVGFLQRGKIRKRS"
BASE COUNT          721 a          680 c          638 g          617 t
ORIGIN      
        1 tgcattatgg aaattcggat ttttgtcggg catttcagat gcgaatccgg cgcgtctgaa
       61 gctaaaaact aaaacgactg cctctggttt gaagttcgac aaactgtttg aagttgccaa
      121 agaaacattt tttttcgcgt cagcctatgt gttgcggcag cagcgcgggc acactgcttt
      181 ctttttgccg cgccgtttcc agaaatatca tatcgacttt cggctcggct tttttcatcg
      241 cttcttcaat ttccgcaatc gtttgaatga cttcattatt cgttaaatcg tcttttaagt
      301 tgacgtgcgc gttgaccaga atttgcttcg gtgcgagatg catcgtcaat aattcgacga
      361 cctcgaccac gttcgggtga gttttgacgg cttgacgaat tttttcgacg tttttcgccg
      421 tcgccgcttc gcccaaaaga agtccacgcg aagagcgtgc caaaccgaaa gcgacgatcg
      481 ccagcacgac gccgatagta atggaagcaa ttccgtccca gagtcttcct tcgtacggcg
      541 gcacgtgata ctgccccaaa agcaagccga gcgccgcaat cacgatgccg agcaaagccg
      601 ccgaatcttc aaagataacg gtctttgccg tcgggtcttt cgattcgcgc aggtattcgc
      661 cgaaagtcat tccctcgtga tgcgcctcgt taatttcctg atacaaagcc agcgcgatcg
      721 aaccggcttc gagcacaaac gaaattccca gaatccagta agcccaaaac agattttccg
      781 gctcgtgcgg gtggcgaact ttttcgatgc cttcgtaaat ggcgtaagtc gcgccgacgc
      841 cgaaaataaa aatcgcggcg ataaacgacc agaaaaagcg ctccttaccg tagccgaaag
      901 gatgtttttc cgacgccgga cgcttgtaaa aacgcagccc gagcagcaga aaaacctgat
      961 tcgtcgtgtc cgccagcgag tgcagcgact ctgccatcat accggacgag ccggttagca
     1021 acgcgccgat aaatttcaaa atcgtgatta aaccatttgc aactaaagcg cctaaaacgg
     1081 caagcattca atagctcctt taaacaaaat aattttattg attatgtgat aaaagcgagg
     1141 taaatcgcaa agaattcgaa ccgatttatt caaggctaaa ggctaagggc taagggcaaa
     1201 taaaatttct tcttctattt tgcctttagc cctttgcttt ttgccttgtt ttaacttctt
     1261 ttcctgattt taccgcgttg cagaaagccg accagctcgc cgttttcgtc aatcacgccg
     1321 agcgcgccga tgccgttggc gcgcatcaat tcgcgagcgt cggcaatcgg cgaattattt
     1381 tcgacaaaat aatcggtcgt cacggggcgc attacttcca gaacctgttt tttattccag
     1441 tcttcgcgcg gaatcttttt caaatccgcc agcgtcagga tgccgtaaag ctgacggtct
     1501 ttcgcgacca gaaaaacgct ctggcggtga accggcagaa tatggtcgat aaaatgcaaa
     1561 acgctggcgt cgggctcgac cgcgaccggc agagtcatcg cttcttcgac cagaaaattt
     1621 tcgtttttgg aagcttgttt aatgattccg gtcgccgcgt ccagcagaaa aagtccgacc
     1681 agaaccgtcc agattccggt aaaaaaatcg ccgcgcacaa atgaaatata caatccgacg
     1741 ctaatcagag cgatggcgat aatctgtccg gcgcgtcccg tcgtgatggt cgcttcgttt
     1801 aaatccgtcc cgcgccgcca caaaaaagcg cgcagaacgc gcccgccgtc gagcggataa
     1861 cccggaaaaa gattgaaaat cgccagcagc atattgccga aaaacaacat cctcaaaagc
     1921 ggcgtcagaa cgtccgtctg gagaaaaagc gaaagcgcgg tgagcagcaa aaacacgagc
     1981 gccagcagaa agctggcgaa aggtccggca atggcgatgc gaaactcggc gcgcggcgta
     2041 tccggctcgc ggcgaaaccg cgccagaccg ccgaacggat gcagaacgat ttccagaacc
     2101 tcgacgcctt cgcgccgcgc cgtaaaagcg tgcgccagct cgtgaaacaa aatcgaagca
     2161 aaaaaaatga gcgtggaaat aaaagctaaa acgagccgcg cgccgaagtt ttcgatcagc
     2221 tcgaccggaa tattgaccgc cgtcaaaacg gaaatcaaaa tcaaaacgcc gaaccatcga
     2281 taatcgaccc gcacgggaat atcgaaaacg tgcgcgacga gaagctggcg tttggaaaac
     2341 tcaaggacgt tcatctttta cctgtaagga taaattatga acgagagcga ttgaaatagt
     2401 ctagcttccg tctagctttt aatttttatt tagattggga attgcttgaa tgttatttga
     2461 gactaacggc tgaaatgacg agggcaaaaa ccggctgagt gcaagccgca ggttcagaag
     2521 aaccactata agaaagtagc tgtttttgcc tcacgtccat tgatttgtta gatgccgcct
     2581 aatcaggaag gtacgcatcg ttttcagcgt agtaaattac cgccgcacat ttatctagtg
     2641 cagttggatt tcttcc
//
LOCUS       DASWNE010000845         3164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_339882, whole genome shotgun sequence.
ACCESSION   DASWNE010000845 DASWNE010000000
VERSION     DASWNE010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339882"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VGB68_16505"
     CDS             complement(<1..363)
                     /locus_tag="VGB68_16505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050896.1"
                     /translation="MNKKSILIKSFVVLVCLISLSLPAAAQKRKTSRRTTPKPVATSV
                     SSSTTTNALEIKNGAEKVSAQLVNVTRFIYLLGGIARNIEDIDKESRSGGTTSRTGGN
                     KVAQNETNKRNVIASIRNL"
     gene            493..1170
                     /gene="lipB"
                     /locus_tag="VGB68_16510"
     CDS             493..1170
                     /gene="lipB"
                     /locus_tag="VGB68_16510"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEY0050897.1"
                     /translation="MNKRPLKVCRLGRIDYDEALALQKQTEQAVIAREAPDTLFLLEH
                     PHTFTLGRSAKMAGVLATAEVLKSLDVSVYETNRGGKVTYHGLGQIVGYPIINLSPDR
                     EDVHKYVRDVEEALIRTMADFDVEAFRIEGLTGVHTRDGKVAAIGVHLKRWVTTHGFA
                     LNVNTDLSFFNWIIACEGEPVSSMEKILGRELMLAEVEDRIIKNFVDVFGFEKYEELQ
                     SAIAVEA"
     gene            1324..2298
                     /locus_tag="VGB68_16515"
     CDS             1324..2298
                     /locus_tag="VGB68_16515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050898.1"
                     /translation="MKFTKLFAFLMAVGIFAACGAPATNTNTNTNGNMRAADNVNATP
                     ATPAVQNLSEVPRPQKIQEMTAQRGEQEQAAPALKIVAPADGSTVNSSTVKVKLNLSG
                     DLKGYKPMKDMATQMGNHIHVILDNQPYEAYYNLEQEFELRNVANGEHTLRVFPSRPW
                     HESYKTEGAFQMVKFTVTNGGANANQPVTTNSGQQMSNANTNANTSAAPEGKDMANST
                     AGTVDKTKPLLTYSRPKGEYKGADAEAIMIDFWLANARLAGDGGEYRVRYSVDGGEAK
                     FIDKWQPIWLSGWTDGKHSVKVELVDKNGNLVDNGGYNSTTREITVAK"
     gene            2508..2828
                     /gene="sugE"
                     /locus_tag="VGB68_16520"
     CDS             2508..2828
                     /gene="sugE"
                     /locus_tag="VGB68_16520"
                     /inference="COORDINATES: protein motif:HMM:NF008512.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HEY0050899.1"
                     /translation="MAWVFLFIAGILEIGWAIGLKYTEGWTRLVPSIVTVAIMVASFY
                     FLSLALKTLPIGTAYAVWTGIGTVGATVLGMFIFGEPRDAARILCVALIIAGIAGLKS
                     TSTQ"
     gene            2907..>3164
                     /locus_tag="VGB68_16525"
     CDS             2907..>3164
                     /locus_tag="VGB68_16525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050900.1"
                     /translation="MRRDTTSWYSHNLNMDMPLVAYGEAGVPLLMFPTAAADYLEYER
                     FLLIDAIKPFIESGRIRAYSINSVNRYSLLNKNESPRRKAEL"
BASE COUNT          826 a          762 c          820 g          756 t
ORIGIN      
        1 caggtttctg atgctggcga taacgtttcg cttgttggtt tcgttctgcg cgactttatt
       61 tccgcccgtc cgcgatgtgg ttccgccgct tctggattcc ttgtcgatgt cttcaatatt
      121 gcgggcgatt ccgcctaaaa gataaataaa tctggtcacg ttgacgagct gcgcggaaac
      181 cttttccgcg ccgtttttga tttcgagcgc gttggtcgtc gtgctggaag aaacggaagt
      241 cgccaccggc ttgggcgtcg tccggcgcga cgttttgcgt ttctgcgccg ccgccggcag
      301 gcttaaacta atcagacaaa ccaaaacaac aaacgatttt attaaaattg attttttatt
      361 cattttttcc tcttaaagat tgccgcccgg caaacgcgtt tgaaatttca aatcagcggc
      421 gcacgagcca gccgccgaac ggcaagtttt tattttcaga tagcatcaag taaaatctta
      481 acataagcaa agatgaataa aagaccttta aaagtttgcc gtctcggtcg cattgattac
      541 gacgaggcgc tcgccctgca aaaacaaacc gagcaagccg ttatcgcgcg cgaagcgccg
      601 gacacgcttt ttctgctcga acacccgcat acctttacgc tcggcaggag cgcgaaaatg
      661 gctggcgtgc tggcgaccgc cgaggtttta aaatctctgg acgtttcggt ttatgaaacg
      721 aatcgcggcg gcaaggtgac gtatcacggg ctcggacaaa tcgtcggcta cccgattata
      781 aatttatcgc ccgaccgcga ggacgtgcat aaatatgtgc gcgacgtcga agaagctttg
      841 attcgcacga tggcggattt cgacgtcgaa gcgtttcgca tcgaaggctt gaccggcgtt
      901 cacacgcgcg acggaaaagt agcggcaatc ggcgttcacc tgaaacgctg ggtgacgaca
      961 cacggcttcg cgctgaacgt caacacggac ctgagctttt tcaactggat tatcgcctgc
     1021 gaaggcgaac ccgtatcttc gatggaaaag attctgggac gcgaactgat gctcgccgaa
     1081 gtcgaagacc ggataattaa aaattttgtc gatgttttcg ggtttgaaaa gtatgaagaa
     1141 ctgcaatcgg caatcgccgt cgaagcctga gaaatcttct ttgcgagctt tgcttctcaa
     1201 ggcagcaaac ccgtttcctg aaaattgata aatgtcggca cagataaaaa gatttctcac
     1261 gcttgacgga caaagctcgc aaagttattt ggtaacaatg ttttaagaag aataaggaga
     1321 attatgaaat ttacgaaatt attcgcattt ttaatggcgg tcggcatttt cgcggcttgc
     1381 ggcgcgccgg cgacgaatac taatacgaac acgaacggaa atatgagagc tgccgataac
     1441 gtcaatgcaa ctccggcgac gccagccgtc cagaatttga gcgaagtacc gcgcccgcag
     1501 aaaatccagg agatgacggc gcagcgcggc gagcaggagc aagccgcgcc ggctttgaaa
     1561 atcgtcgcgc ccgccgacgg ctcgaccgtc aatagctcga ccgtcaaagt caagctcaat
     1621 ctgagcggag atttaaaagg ctataagccg atgaaggata tggcgacgca gatgggcaat
     1681 catattcacg ttattctcga caatcagccg tatgaagcct attacaatct agaacaggaa
     1741 ttcgagctga gaaacgtcgc caacggcgag cacaccctgc gcgttttccc gtcgcgcccg
     1801 tggcacgaaa gctataaaac ggaaggcgcg tttcagatgg tgaaatttac cgtgacaaac
     1861 ggcggcgcga atgcaaatca gccggttacg acgaattcgg gacagcaaat gtcgaacgcc
     1921 aatacaaatg ctaatacttc agccgcgccc gaaggcaagg atatggcaaa ttcgactgcc
     1981 ggcacggtcg ataaaacgaa accgcttttg acctacagcc gcccgaaagg cgaatataaa
     2041 ggcgcggacg ccgaggcgat tatgattgat ttctggctgg caaacgccag gctcgccggc
     2101 gacggcggcg aatatcgcgt gcgctactcg gttgacggcg gcgaggcgaa atttatcgac
     2161 aagtggcagc cgatttggct ttccggctgg acggacggca aacacagcgt caaggtcgaa
     2221 ctggtcgata aaaacggaaa cctcgtggac aacggcggtt ataattcgac gacgcgcgaa
     2281 attacggtcg ctaaataatt ggaaacttgt tttgagtttt tcctcgaaca atcggtatcc
     2341 gaaataatcg gataccgttt aaatactcga tacaaaaaca ttcattttgc cgggcacatt
     2401 taaaaacgat aacctgaaca ggctgcgata aacgcacgac tatatagaga cggctttata
     2461 tagcaatcga gattttggga cgacccgaac cttatttgga gaaaaatatg gcttgggtct
     2521 ttctttttat cgcgggaata cttgaaatcg gctgggcaat cggcttgaaa tacacggaag
     2581 gctggacgcg tcttgtgccg agcattgtca cggtcgcgat aatggttgcg agtttttatt
     2641 ttctttcgct tgcgcttaag actttaccaa tcggcacggc ttatgccgtt tggacgggaa
     2701 tcggcacggt cggcgcgact gttttgggaa tgtttatttt cggcgagccg cgtgacgcag
     2761 cacggatttt gtgtgtcgcg ctaatcatcg ccggaatcgc cgggttaaaa tcaacttcga
     2821 cgcagtgaaa ttttatattc tttcgttata aaatcgaaaa gttttataca atctgaattt
     2881 aacaactttt tggagctgaa tattttatgc gacgtgatac gacttcgtgg tatagccaca
     2941 atctgaatat ggatatgccg ctggtagctt acggcgaggc gggcgttccg cttttgatgt
     3001 ttccgaccgc tgccgccgat tatctcgaat acgaaaggtt tttactgatc gacgcgatca
     3061 agccgtttat cgaaagcggt cgcattcgtg cgtattcgat caactcggtc aaccgctaca
     3121 gccttctgaa caaaaacgaa tcgccgcgcc gcaaagccga gctt
//
LOCUS       DASWNE010000846         1614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_98838, whole genome shotgun sequence.
ACCESSION   DASWNE010000846 DASWNE010000000
VERSION     DASWNE010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98838"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1090)
                     /locus_tag="VGB68_16530"
     CDS             complement(<1..1090)
                     /locus_tag="VGB68_16530"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0050901.1"
                     /translation="MMKELSLQNSRLDGRYDITSLLGRGSYAEIYVARDTLAAPDSPH
                     KQVVVKALNVFMQNDLDADLERTLVENFQNEAIALDRVRHPNVVSRLGHGTARDLRGT
                     VFHYLVLEYLTGGDLARLCRQKSINLLQALKYIEQVCAGLGHAHKNGVIHRDIKPQNL
                     LLTEDRETVKIADFGVARFSASDSPITRVGTNIYAPPEHSPMVTDDGNVATVTELTPA
                     ADVYSLAKSAYVLITCESPRFFANQPVKELPFDARREIWADDLLRVLQKATQNNPKDR
                     YQTVDEFWKDLSRIKLLAEPGRREIKPEVSSPQSASPRAHVAEGYTPLAPLRPQFNTS
                     RDLKLKNLGIPENAPNLVVKLDSNYQPPA"
     gene            complement(1292..>1614)
                     /locus_tag="VGB68_16535"
     CDS             complement(1292..>1614)
                     /locus_tag="VGB68_16535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050902.1"
                     /translation="NIEGTPNQGGISPKLNGGVHNFKRFLERWTDPSNSAFRTYLNYS
                     GSLINLFNSRNNNGAFKCCNTVYNPPVRNWVFDSTFLDASRLPPGTPFFQYVQITGFQ
                     RTND"
BASE COUNT          372 a          405 c          470 g          367 t
ORIGIN      
        1 gtgccggcgg ctggtaattc gaatccagct tgaccaccag attgggagcg ttttccggaa
       61 tgccgagatt tttcagcttc aaatcgcgcg aagtgttgaa ttgcgggcgc agcggcgcca
      121 gcggcgtgta gccttcggcg acgtgcgcgc gcggcgaagc ggattgagga gaagaaacct
      181 ccggcttgat ttcgcgtctg cccggttcag ccaaaagttt tatgcgggat aaatctttcc
      241 agaattcgtc gaccgtctga tagcggtctt tcgggttgtt ttgcgtggct ttttgcagaa
      301 cgcgcagtaa atcgtccgcc cagatttccc ggcgcgcgtc gaagggaagc tccttgaccg
      361 gctgattggc gaaaaagcgc ggcgattcgc aggtaatcag gacgtaagcc gattttgcca
      421 gcgagtaaac gtcggcggcg ggcgtcaatt ccgtgacggt cgccacgttt ccgtcgtcgg
      481 tcaccatcgg gctgtgttcg ggcggcgcgt agatatttgt gccgacgcgc gtaatcggcg
      541 aatcggaagc ggaaaagcga gcgacgccga aatcggcgat ttttaccgtt tcgcggtctt
      601 cggtcaaaag caggttctgc ggcttgatgt cgcggtgaat gacgccgttt ttatgcgcgt
      661 gtccgagccc ggcgcaaacc tgctcgatgt atttcaaagc ctgcaaaagg ttgatgcttt
      721 tctggcggca cagtcgcgcc aaatcgccgc ccgtcagata ttccaaaacc agataatgaa
      781 aaaccgttcc gcgcaaatcg cgcgccgtgc cgtgaccgag gcggctgacg acgttcgggt
      841 gacgaacgcg gtcgagcgcg atggcttcgt tctgaaaatt ttcgaccagc gtccgttcca
      901 aatccgcgtc caaatcgttt tgcataaaaa cgttgagagc tttgacgaca acctgtttgt
      961 gcggcgaatc aggcgcggcg agcgtgtcgc gagcgacata aatttcggca tagcttccgc
     1021 gtccgagcag agaagtaata tcgtatcgcc cgtcgagccg cgagttttgc aaggaaagtt
     1081 ctttcatcat caatcaagta aatgctacat caaaatgcgg gacttcacca ataaactgat
     1141 acgaaagaag cgagcgaaag ttcaatttcg caagaagacg gaagacagaa gacggaagtc
     1201 aaaataaaag acggaagccg gaaggcggtc agcagtaaat aaactggctg cctttcgact
     1261 tccgtcttcc gacttccgtc ttctgttttc cttagtcgtt cgtgcgctga aatccggtta
     1321 tctgcacgta ctggaaaaac ggcgtgccgg gcggcaggcg cgaagcgtcc agaaacgtgc
     1381 tgtcgaatac ccagttgcga accggcggat tgtaaaccgt gttgcagcat ttaaacgcgc
     1441 cgttgttgtt gcgcgaattg aaaagattaa tcagggaacc ggaataattc agataagtcc
     1501 tgaaagcgga attggacggg tccgtccatc tttccagaaa gcgcttgaag ttgtgaacgc
     1561 cgccgtttaa tttcggcgaa atgccgccct gattcggcgt gccttcgata ttgg
//
LOCUS       DASWNE010000847         3926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_240367, whole genome shotgun sequence.
ACCESSION   DASWNE010000847 DASWNE010000000
VERSION     DASWNE010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240367"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2463)
                     /locus_tag="VGB68_16540"
     CDS             complement(<1..2463)
                     /locus_tag="VGB68_16540"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY0050903.1"
                     /translation="MTKLFTNIFSALLISALLLTSVVQGQTLAPQSNAKNLEKSASQS
                     EVFPKYVEDLSSLSKQGRTTPAAEGLESEIEKVKAAFSLKEKKGTILVDKTRTKSRAV
                     VEMVAAWMRTETAPLGLRQKKILRLDLPSLLNDAQTEAEAVALLQNTLKSVETANAIL
                     FIEDVSTLSKNNPMFGFAVADVIREAMDGSKLQVVTNGTPDDYKLEVVADFGLRKQLR
                     KIDLDEAGEIIGNDGFVGDKISPDLQALLGTDPNKMVKVILQSDDIDNNELQAVLKRN
                     NVAIESRAESLNMLVLELPARVAEEIAAVQSAKHLSLDLEMKVLGHIDTTTGVLAAKT
                     QLGNSILDGRGIGIAIVDSAVFDAHHAFVGANGNKRIAEHKEFVSGGSEDKFGHGTHV
                     AALAAGRGGKPGDNNSINVLKNYQGVAPEAKIIAVRALDNEGRGSTAKLIEALNWIFT
                     NRAKHNIRVVNLSLGTAAVETWRNDPVCRAVRNLTAAGVVVVAAAGNNGKDAQGNKIY
                     GAIHAPGNDPSVITVGAANTFGTDKRDDDAIATFSSRGPTRSFYTENGVKKYDHLIKP
                     DLVAPGNKIIAARAKDNELAEGNPQLEINGDGNNDDYQLMYLSGTSMATPIVSGTVAL
                     MLQANPKLTPNMVKMILQYTAQPLAGFNTLEQGAGNLNVEGAVRVAKLVRQDLTATTP
                     LGTPMLTSGALLPNHTSTIGGTRFQWSGGVTAGYGLLTGTELISEYQKVYANGLLLSD
                     GLLLSDGLLLSDGLLLSDGLLLSDGLLLSDGLLLSDGLLLSDGLLLSDTTLVANSSAL
                     FSSTAQLKGGTLFMPAGNLYNSN"
     gene            complement(2727..>3926)
                     /locus_tag="VGB68_16545"
     CDS             complement(2727..>3926)
                     /locus_tag="VGB68_16545"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY0050904.1"
                     /translation="TNKNAENFRIVRAPVADPSEKNWKEFVPHNPSIKIDGVDFFKDY
                     AVLSELENGLEYLKVLDLKTKRAPRRIATPESVYTMNMWVNPEFDTPVIRYTYASMIT
                     PNSTYEYNFKTRESEILKQQEIPSGYDKTKYETTRVWATARDGVKVPVSVMMEKGTKL
                     DGAAPMLLYAYGSYGISMTPSFSTNRLSLVDRGMIYAIAHIRGGSELGEKWRQEGRMM
                     KKLNTFHDFIDSAKWLIANKYTSPDRLVIQGGSAGGLLMGAVVNMSPETFKAAIVQVP
                     FVDVMNTMLDDSLPLTTGEWIEWGNPNEKEAFDYMIKYSPYENLKAQNYPNMLIETSL
                     YDSQVMYWEAAKYAAKLRAVKTDDNIVMLKTNMGAGHGGSSGRFDRLKEIAFDYAYAL
                     NQVGITK"
BASE COUNT          800 a         1071 c         1049 g         1006 t
ORIGIN      
        1 gttgctgtta tacagattgc cggcaggcat aaagagcgtg ccgcctttca gctgcgccgt
       61 cgaactgaac aacgccgagg aattggcaac cagcgtggta tcgctgagca gcaagccatc
      121 gctcagcaat aaaccgtcgc tcagcagcaa gccgtcgctc agcaatagac cgtcgctgag
      181 cagcaaaccg tcgctcaata acaagccgtc gctgagcaat aaaccgtcgc ttaataaaag
      241 accatcgctg agcagcaagc cgttggcata gactttctgg tattcagaaa taagctccgt
      301 gccggtcagc aaaccgtagc ccgccgtcac tccgccagac cactggaagc gcgtgccgcc
      361 gatggtcgaa gtgtgattcg gcagtaatgc gccggaggtc agcatcggcg tgccgagcgg
      421 agtcgtcgcg gtcaagtcct gacgaacgag ttttgccacg cgaaccgcgc cttcgacgtt
      481 cagattgccc gcgccctgtt cgagcgtgtt gaagcccgcg agcggctgcg ccgtgtattg
      541 cagaatcatc ttgaccatat tcggcgtcag cttcggattg gcttgcagca ttaaagcaac
      601 cgttccggaa acaatcggcg tcgccatcga agtgcccgac agatacatca actggtaatc
      661 gtcattgttg ccgtcgccgt tgatttccaa ctgcggattt ccctcggcta gctcgttatc
      721 cttagctctg gcggcaatta ttttattgcc cggcgcgacc aaatcgggtt tgatgagatg
      781 gtcgtatttt ttcacgccgt tttcggtgta gaacgagcgc gtcggaccgc gcgagctgaa
      841 cgtcgcaatc gcgtcgtcgt ctcgcttgtc agtgccgaaa gtgttggctg cgccgaccgt
      901 gatgaccgac gggtcgttgc ccggagcgtg aatcgcgccg tagattttgt ttccctgcgc
      961 gtctttcccg ttgtttccgg cggcggcgac gacaacgacg ccggcggcgg tcaagttgcg
     1021 gacggcgcgg caaaccgggt cgttgcgcca cgtttcgacc gccgccgtgc cgaggctcag
     1081 gttgacgacg cgaatattat gtttggcgcg gttggtgaaa atccagttaa gcgcttcgat
     1141 gagcttggcg gtcgagccgc gtccttcgtt gtcgagcgcg cgaaccgcga tgattttcgc
     1201 ttccggcgcg acgccctgat aatttttcag aacgttgatg gaattgttgt cgcccggttt
     1261 gccgccgcga ccggcagcga gagcggcaac gtgcgttccg tgtccgaatt tatcttccga
     1321 accgccgctg acgaattctt tatgttcggc gatgcgtttg tttccgttcg cgccgacaaa
     1381 agcgtgatga gcgtcgaaaa ccgccgagtc aacaatggcg atgccgatgc ctctgccgtc
     1441 aaggatgctg ttgccgagct gtgttttagc cgccagcacg ccggtcgtcg tgtcgatgtg
     1501 accgagaacc ttcatttcca ggtcgagcga aagatgtttg gcgctctgca cggcggcgat
     1561 ttcttcggcg acgcgcgccg gcagttccag cacgagcata ttcaggcttt cggcgcggct
     1621 ttcgattgcc acgttgttgc gttttaagac cgcctgcaat tcgttgttgt cgatgtcgtc
     1681 cgattgcaga atgactttca ccatcttgtt cgggtcggtg ccgagcagag cctgtaaatc
     1741 gggcgagatt ttatcgccga caaagccgtc gttgccgatg atttcgcccg cttcgtccaa
     1801 atcgattttg cgaagctgtt tgcgcaagcc gaaatcggcg acgacttcca gtttgtaatc
     1861 gtccggcgtt ccgttggtca cgacctgaag cttactgccg tccatcgcct cgcgaatcac
     1921 gtcggcgacc gcgaaaccga acatcggatt atttttggaa agcgtcgaaa cgtcttcgat
     1981 aaataaaatg gcattcgccg tctcgaccga ttttaaagtg ttttgcaaaa gcgcgaccgc
     2041 ttccgcttcg gtttgcgcgt cgtttaaaag cgacggcaaa tcgagccgca gaattttctt
     2101 ctgccgtaag ccgagcggag ccgtttcggt tctcatccac gcggcgacca tttcgacgac
     2161 cgcgcggctt ttcgtccgcg ttttatcgac cagaatcgtg ccttttttct cttttaagga
     2221 aaaagccgct ttgacttttt cgatttcgct ttcgagacct tccgcggcgg gcgtcgttct
     2281 tccctgtttc gacagggatg aaaggtcttc aacatatttt ggaaaaactt cggattggct
     2341 cgcgcttttt tccaaatttt tcgcattact ttggggagct aacgtctgcc cttgaacgac
     2401 cgaagtcaga agaagcgcgg agattagcaa tgccgaaaaa atgtttgtga ataattttgt
     2461 catttctttt cctctattcg tttcaccctg caaaataaaa atatttcgcg cctttctttt
     2521 ggcaacgccc gtgccagaag cgaatatttg ttattagaca gcaaacatcg aggttttaat
     2581 ataaattgaa acaagtcggt catctgcaaa agacctgcgt cggtcaattt gaacgacttt
     2641 ttctcaattt gaccacaaag caaaacggcg aaagaccttt cggtcaatcg ccgtcgtcgt
     2701 aaaaataacc gccggattcg ccgcttttat ttggtaattc cgacctgatt cagcgcgtat
     2761 gcgtaatcga aggcaatctc cttcaaacgg tcgaacctgc cgcttgagcc gccgtgtccg
     2821 gcgcccatat tggttttgag cataacgata ttatcgtccg tttttacggc gcgcagcttt
     2881 gccgcgtatt tcgccgcttc ccaatacatc acctggctgt cgtagagcga cgtttcgatg
     2941 agcatattcg gatagttctg cgccttcagg ttttcatacg gcgaatactt aatcatataa
     3001 tcgaaggctt ccttttcgtt cggattgccc cattcgatcc attcgccggt cgtcagcggc
     3061 agcgaatcgt cgagcatcgt attcatcaca tcgacaaacg gaacctgcac gattgccgct
     3121 ttgaaagtct ccggcgacat attgaccacc gcgcccatca aaagaccgcc cgcgctgccg
     3181 ccctgtatca ccagacggtc cggcgaagtg tatttgttgg caatcagcca tttggcggag
     3241 tcgatgaaat cgtgaaacgt gttgagcttt ttcatcatcc tgccttcctg tcgccatttt
     3301 tcgccgagct ccgagccgcc gcgaatgtgc gcgatggcgt aaatcattcc tctatcgacc
     3361 agactcaggc gattggtcga aaagctcggc gtcatcgaga tgccgtaaga gccgtaagcg
     3421 taaagcagca tcggcgcagc tccgtcgagt ttggtgccct tttccatcat caccgaaacg
     3481 ggaactttca cgccgtcgcg cgccgtcgcc cagacgcgcg tcgtctcgta tttcgttttg
     3541 tcgtagccgc tgggaatttc ctgctgtttg agaatctcgc tttcacgggt tttgaaattg
     3601 tattcgtagg tcgagttcgg cgtaatcatc gaagcgtagg tgtaacgaat gacgggcgtg
     3661 tcgaattcgg gattgaccca catattcatc gtgtagacgc tttccggcgt ggcgatgcgg
     3721 cgcggcgcgc gctttgtttt caaatccaga accttcaggt attccagacc gttttcgagc
     3781 tccgaaagga cggcgtaatc tttgaaaaag tcgacgccgt cgatttttat cgaaggattg
     3841 tgcggcacga attccttcca gtttttctcg gacgggtcgg cgaccggcgc gcgcacgatg
     3901 cgaaagtttt cggcgttttt gttggt
//
LOCUS       DASWNE010000848         3219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_487653, whole genome shotgun sequence.
ACCESSION   DASWNE010000848 DASWNE010000000
VERSION     DASWNE010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487653"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="VGB68_16550"
     CDS             <1..1167
                     /locus_tag="VGB68_16550"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0050905.1"
                     /translation="APDFSGVLTSQGYNLFGNTAGTTITGFGTGNLVNVNALLDPILR
                     DNGGSTRTHALLPNSPAIDKGSPLSPALLIDQRELTRPIDFPSIPNALNGNGSDIGAF
                     ERQTVEILRKTRFDYDGDGKADLSVFRPNTGSWYLSHSSNNAFIAVQFGANGDLIAPA
                     DFDGDGKTDICVFRPSDGGWYRLNSSNNTFTPAQFGTNGDLPVPGDFDGDGKADLTVY
                     RPSAGSWYRINSSNNQFVAAQFGVAEDKPLVGDFDGDGKSDLTVFRPSNGTWYRINSA
                     TDTFSPAQFGAPGDLPAAADYDGDGKTDLAVYRPSVGDWYIINSSNGSFTGLHFGVTE
                     DKPAPADFDGDGKADLVVFRPSSGTWYLLRTTAGFTGFQFGASGDVPTPNAFVR"
     gene            1452..3170
                     /locus_tag="VGB68_16555"
     CDS             1452..3170
                     /locus_tag="VGB68_16555"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEY0050906.1"
                     /translation="MKNIIILFVVFVFFVSPIASAPKADKKQERWVEKTLKSMSLREK
                     IGQMINVRLVGDFTNFQSERFADYRREVEQNKIGGFTVYRGDANTIANLTNELQRISK
                     VPLFFSADYERGLRMNVRNGTPFTTNMGVGAAGDVQAAYRQGKIICEEMRAIGVNWLF
                     APVVDINNNPDNPVINVRSYGENPQKVGEFGSALAKGVKDAGCLATLKHFPGHGDTAT
                     DSHIGLSIVPIDKARLDAVELVPFKMAIQSGVDSVMTAHVALPQITNDEIPSTLSAKV
                     STDILRRELKFDGIISTDAMEMGAIIKTYSEEKSVVMAVQAGADLILLPLNSTSAINA
                     VEAAVKRNEITEQRIDESVRRLLKAKYRIGLTQNTQVDLTKVNQLVENPLNVREANSV
                     AEKSITLLRNANGMLPLNIEKANRTLFVVAAADDDPVEGAAFMPEVQKRAPNAKVLRL
                     DFRTTREEFEKVSADAQNFDSIVLAVFVKRAASKGTVALPENLTGFVQQMVTIKDKNV
                     AVVAFGSPYLIRQFPEARVYAVTYAIEEVGQNAAVRTLFGEVPFQGKLPVRIPNLFEI
                     GAGITK"
BASE COUNT          804 a          840 c          842 g          733 t
ORIGIN      
        1 gcgccggatt tttccggggt gcttacctcg caaggttaca acctcttcgg caacacggcg
       61 ggaaccacaa ttaccggctt cggaaccggc aatttagtta acgtcaacgc gctcttagac
      121 cctattttgc gcgataacgg aggctcgacg cggacacacg ctctgctgcc caacagtccg
      181 gcaatagaca aaggcagccc gctgtcgcca gccttattaa tcgaccagcg cgaactgact
      241 cgtcccattg atttcccgtc aattccaaat gctttgaacg gcaacggctc ggacatcggc
      301 gcttttgaac ggcagacagt cgaaattctg cgaaaaaccc gtttcgatta tgacggcgac
      361 ggcaaagcag acctttcggt attccgtccg aatacgggaa gctggtattt atcacacagc
      421 tcaaacaacg cttttattgc cgttcaattc ggcgcaaacg gagatttaat cgcacccgcc
      481 gattttgacg gcgacggcaa aaccgacatc tgtgtgtttc gtccttctga cggcggctgg
      541 tatcgtttga attcttcaaa caacactttt actccggcac aatttggaac aaacggagat
      601 ttacctgtgc cgggtgattt cgacggcgac ggcaaagcgg atttgacggt ttatcgaccg
      661 tcggcgggaa gttggtatcg catcaatagc tcgaataatc agtttgtcgc cgcacagttc
      721 ggagtggcgg aagacaaacc tttagttgga gattttgacg gcgacgggaa atctgattta
      781 actgttttcc gtccttcaaa cggcacgtgg tatcgcatca acagtgcaac cgatacattt
      841 tctccggcac agtttggcgc gcccggagat ttgcccgcgg cggctgatta cgacggcgac
      901 ggcaaaacgg atttagccgt ctatcgtccg tctgtcggag attggtacat tatcaacagc
      961 agcaacggtt ctttcaccgg actgcacttc ggcgtgacgg aagacaaacc cgcgccggct
     1021 gatttcgacg gcgacggaaa agccgacttg gttgttttcc gtccgtcgtc cggaacctgg
     1081 tatctgctcc gaacaacagc cggctttacc ggatttcagt ttggcgcaag cggcgatgtt
     1141 ccgactccga acgcttttgt caggtaagga aatatttcca tttaaaggct ttttttcaat
     1201 ttggaaaaaa gcctttaaat gagtttttta gaaaaaacgt cgaaagtaaa cgaagccgac
     1261 tgaatcaagc cgtttaatcg tcaatctcgc acaaacagtt tcggaacagg caaagcctgt
     1321 ttataaaaga ttccgaatca gacgcgtcac agtttctttc tctccttctc cttttctccc
     1381 tttctcctct tcttctttcg cgcccttgcc ccgcgcgcgg gcttctgcct acaatactca
     1441 agaggtcagt cgtgaaaaat ataataattt tattcgtcgt tttcgttttc tttgtttcgc
     1501 ctatcgcttc cgcgccgaaa gcggacaaaa aacaggaaag atgggttgaa aaaactctta
     1561 aatcaatgag cctgcgcgag aaaatcgggc agatgataaa cgtccggctg gtcggcgatt
     1621 tcacgaattt tcagagcgaa agattcgccg attaccggcg cgaggtcgaa cagaacaaaa
     1681 tcggcggttt tacggtttat cgcggcgatg cgaatacgat tgccaatctc accaacgagc
     1741 ttcagcgaat ttcgaaagtt ccattattct tttccgccga ttacgagcgc gggctgcgta
     1801 tgaacgtccg caacggcacg cctttcacga cgaatatggg cgtcggcgcg gcgggcgacg
     1861 tgcaggcggc gtatcgacag ggaaaaatta tttgcgaaga aatgcgcgcc atcggcgtca
     1921 actggctttt cgcgccggtc gtagacatca acaataatcc cgacaatccg gtgataaatg
     1981 tccgcagcta cggcgaaaat ccgcagaaag tcggcgaatt cggctcggcg ctggcgaaag
     2041 gcgtcaagga cgccggctgt ctggcgactt taaaacattt tcccggtcac ggcgacacgg
     2101 cgaccgattc gcacatcggg ctttcaatcg tgccgattga taaagcgcgg ctcgatgcgg
     2161 tcgagctggt gccgtttaaa atggcgattc agagcggcgt cgattcggtg atgacggcgc
     2221 acgtcgctct gccgcaaatc acgaacgacg aaattccttc gactttaagc gcgaaagtct
     2281 cgaccgatat tctgcgcagg gaattaaaat ttgacggaat tatctcgacc gacgcgatgg
     2341 aaatgggcgc gattatcaaa acttactcgg aggaaaaatc ggtcgtgatg gctgtgcagg
     2401 cgggcgctga cctgattctt ctgccgctta attcgaccag cgcgataaac gccgtcgaag
     2461 ccgccgtcaa gcgcaatgaa atcaccgaac agcgcatcga cgaatcggtc aggcgcttgc
     2521 tgaaagccaa ataccggatt ggcttgacgc aaaacacgca ggtcgatttg acgaaagtca
     2581 accagctcgt ggaaaatccc ttaaacgtcc gcgaggcgaa ttccgtcgcc gaaaaatcaa
     2641 tcacgctttt gcgcaacgcg aatggaatgc tgccgctaaa tatcgaaaaa gcgaaccgga
     2701 ctttattcgt cgtcgctgcc gccgacgacg acccggtcga aggcgcggcg tttatgccgg
     2761 aagtgcaaaa acgcgcgccg aacgcgaaag tcctgcggct cgattttagg acgacccgag
     2821 aagagtttga aaaagtttca gccgatgcgc aaaattttga ttcaatcgtt ctggcggttt
     2881 tcgtcaagcg cgcggcgagc aagggaaccg tcgcgctgcc ggaaaatctg acgggctttg
     2941 tgcagcaaat ggtgacgata aaagataaaa acgtggcggt cgtcgccttc ggctcgccgt
     3001 atctgattcg ccagtttccc gaagcgcgcg tttacgccgt tacttacgcc atcgaggaag
     3061 tcgggcagaa cgccgccgtc aggacgctgt tcggcgaagt tccgtttcag ggaaaattgc
     3121 ccgtgcgcat tccgaacctg ttcgagatcg gcgcgggaat tacgaaataa atcagcccgc
     3181 gaaacacgga aagaatgaga ataagaatcg acgaaatca
//
LOCUS       DASWNE010000849         2615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_664618, whole genome shotgun sequence.
ACCESSION   DASWNE010000849 DASWNE010000000
VERSION     DASWNE010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664618"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2492
                     /locus_tag="VGB68_16560"
     CDS             <1..2492
                     /locus_tag="VGB68_16560"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HEY0050907.1"
                     /translation="RLEYDFVVSPGSNPKQIALKFEGAKKLEIGAEGDLLLHLEDGLV
                     KQNKPVIYQEINGERKEIAGKYVLKNETQVGFQIDEYDISQSLVIDPVLAYATYLGSS
                     EYEISHGIAVDAAGNSYVTGYAIPPTFPTTPGSVRPAVNSDNLTPFVAKFNPTGSALV
                     YSTYLGGAGEGIAVDAAGNAYTTGYPHPELFTTTPGALDLGRGGVGITKLNPQGNQRI
                     YAARFGSSGSDLPREIAIDSQGNAVVVGSAGCFTSCDFPILNAPQPTPPTQGGGGFVA
                     KLNSTGSALVYSTYLGTGTEEIWGVDVDPAGNAYVTGNTTSFNFPTTPGAFDRTFACP
                     EPPFCNPDSFVTKIPPAGGAFAYSTYLGGRGGERGWAIAVDDAGNAYAAGYTDGRFSG
                     FPDEGFPTTPGAFKMFGSVEAYVTKLNPAGSALVYSTLLGGEGTSCGEERARDIDVDR
                     LGNAYVVGLTTCNNFPVANALQSAPSSFQPEGFVTKFNPAGSGLVYSSYLGGPRYDEA
                     YNVAVDTTGAAYITGTTSGDFQLITPGAFDTTWNCGQFCEHPDDIFVMKINGELAPLR
                     NKIADFDGDGRSDISVFRNGNWYLQQSQAGFAATQFGISTDRLAPADYDGDGKTDIGV
                     YRGGNWYYLQSSNNQFRAVQFGVSDDIPQAGDYDGDGKADFAVFRPSNGTWYLQQSAA
                     GFSGIQFGQTGDRPVSADYDGDGKFDAAVYRADTWYVLQSRDGFRAVQFGVSTDTPVV
                     GDYDGDGKSDFAVYRSGVWYLQRSAQGFAAIQFGVASDVPTPADYDGGGKTDIAVFRN
                     GTWYLQQSTAGFASAQFGQENDVPVPSASVP"
BASE COUNT          565 a          713 c          762 g          575 t
ORIGIN      
        1 gccggttgga atatgatttc gtcgtttcgc ccggctctaa tccgaagcaa atcgcgctta
       61 aattcgaagg cgcgaaaaag ctcgaaatcg gggcggaagg cgatttgctg ctgcatcttg
      121 aggacggttt ggtcaaacag aacaagcccg ttatttacca ggaaataaac ggcgagcgta
      181 aggaaattgc cggaaaatac gttttgaaaa acgagacgca agtcggtttt cagattgatg
      241 aatacgacat ctcgcaaagt cttgtaattg acccggtgct cgcttatgcg acttatctgg
      301 gaagcagcga atacgaaatc agtcacggca tcgcggtcga tgcggcggga aattcatacg
      361 tcacaggtta cgccattccg ccgacttttc cgaccacgcc cggctcggtt cgcccggcgg
      421 ttaattccga caatctgacg ccgtttgtcg ccaaatttaa tccgacgggc agcgcgctgg
      481 tgtattcgac gtatctcggc ggcgcgggcg aaggcatcgc ggtcgacgcg gcgggcaatg
      541 cctatacgac cggctatccg catccggagt tatttacgac gacgcccggc gcgctcgatt
      601 taggcagagg cggagtcggc atcacgaaac tcaacccgca gggaaatcaa cgaatttacg
      661 ccgcgcgttt cggcagctcc ggaagcgacc ttccgcggga aattgcgatt gattcacagg
      721 gaaatgcggt cgtcgtcggt tcggcgggct gttttacgag ctgcgatttt ccgattttga
      781 acgcgccgca gccgacgccg ccgacacaag gcggaggcgg tttcgtcgcc aagctgaact
      841 cgacgggttc ggctctggtt tactcgactt atctcggcac cggaacagag gaaatctggg
      901 gcgtcgacgt tgacccggcg ggaaacgctt atgtgaccgg caatacgact tcgttcaatt
      961 ttccgaccac gcccggcgct ttcgaccgaa cttttgcctg cccggaaccg cctttctgca
     1021 atcctgacag ctttgtgact aaaattccgc ctgccggcgg cgcgttcgct tattcgactt
     1081 atctcggcgg caggggaggc gagcgcggct gggcaatcgc ggtcgacgac gccggcaacg
     1141 cgtatgcggc gggatatacg gacggaagat tttccggctt tcccgacgaa ggatttccga
     1201 cgacgcccgg cgcatttaaa atgttcggct cggtcgaagc ctatgtgaca aagctgaatc
     1261 cggcaggctc ggcgctcgtc tattcgacct tgctcggcgg cgagggtacg agctgcggcg
     1321 aggaaagagc gcgcgatatc gacgtcgacc gtctgggaaa cgcttacgtc gtcggcttga
     1381 cgacctgcaa caattttccg gtggcgaacg cgctgcaatc tgcgccgagc agttttcaac
     1441 ccgagggctt cgttacaaaa tttaatccgg cgggcagcgg gctcgtttat tcgtcgtatc
     1501 tgggcggacc gcgttacgac gaggcataca acgtcgcggt cgatacgacc ggcgcggcat
     1561 acatcaccgg cacgacatcg ggcgattttc aattgataac gcccggcgct tttgatacga
     1621 cctggaactg cgggcaattt tgcgaacatc cggacgacat ttttgtgatg aaaatcaacg
     1681 gcgagctggc tcctttgcga aacaaaatcg ccgatttcga cggcgacggg cgctcggata
     1741 tttccgtctt ccgcaatgga aactggtatc tccagcaaag tcaggctggc tttgcggcga
     1801 cgcagttcgg aatctcgacc gaccgccttg cgcccgccga ttacgacggc gacggcaaaa
     1861 ccgatatcgg ggtctatcgc ggcggcaact ggtattacct gcaaagctcg aacaatcaat
     1921 tccgagccgt gcagttcggc gtgtcggacg atattccgca agccggagat tacgacggcg
     1981 acggcaaggc ggatttcgcc gttttccgtc cttcgaacgg aacctggtat ctacagcaga
     2041 gcgcggctgg attttcggga attcaattcg ggcaaacagg cgatagaccg gtttccgcgg
     2101 attacgacgg cgacggcaag ttcgatgccg ccgtctatcg cgccgatacg tggtacgttt
     2161 tgcaaagccg cgacggtttt cgcgctgtgc agttcggcgt ttcaaccgac acgcccgtcg
     2221 tcggcgatta tgacggcgac ggaaaatccg acttcgccgt ttaccggagc ggcgtctggt
     2281 atttgcagcg aagcgcgcag gggtttgcgg caattcaatt cggcgttgct tccgacgtgc
     2341 cgacgccagc cgattacgac ggcggcggca aaaccgatat cgccgttttc cgtaatggga
     2401 cgtggtatct gcagcaaagc accgccgggt ttgcgagcgc gcaattcgga caggaaaacg
     2461 atgttcccgt tccatcggca agcgtgcctt agcgattact aaaatttaga gcggttttct
     2521 aatatatttg ccggaactcc gaaatagata aaagataaaa gatgataacc gtcgctttca
     2581 tcttttaact tttatctatt cgattcgcct ttttt
//
LOCUS       DASWNE010000850         1502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_205948, whole genome shotgun sequence.
ACCESSION   DASWNE010000850 DASWNE010000000
VERSION     DASWNE010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205948"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VGB68_16565"
     CDS             complement(<1..650)
                     /locus_tag="VGB68_16565"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEY0050908.1"
                     /translation="MKRFPIISIIARSQLLYALIAIFVVISIGAFGFHLIEGFSVLDS
                     IYLATETVTTVGYGDLSPKSTTGRVFAIFFMLVGAGTVLYALTSLVQTLFQAELIEML
                     DSRRKIKEMEKLQNHYIVCGAGRVGKRIIRSLQKQNIPFVVIEREERKGAPVADYCDY
                     VIIGDATLEETLKRAGVERAKGLASCLADDAANVYVVLTARGLNGNMHIVARAVEE"
     gene            828..1253
                     /locus_tag="VGB68_16570"
     CDS             828..1253
                     /locus_tag="VGB68_16570"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY0050909.1"
                     /translation="MTENPKCCAPDTSLQEVAKMMVEIDCGCIPVVDENSKPIGMITD
                     RDITVRTVAEGKNPLDLTAGKVMTSDVYTVTPETSIEECCRLMEKKQVRRIAVVDSQG
                     ICCGMVAQADVANYASRKQTAEVVQEVSKSPAFSQSATT"
BASE COUNT          411 a          361 c          388 g          342 t
ORIGIN      
        1 tcttcttcga cggcgcgggc gacgatgtgc atatttccgt ttaagccgcg cgccgtcagc
       61 acgacgtaaa cattcgccgc gtcgtcagcc agacagctcg ccaagccttt cgcgcgctcg
      121 actccggcgc gtttgagcgt ctcttcgagc gtcgcgtcgc cgatgatgac gtaatcgcag
      181 taatcggcga cgggcgcgcc ttttcgctct tcgcgctcga tcacgacaaa cggaatgttc
      241 tgcttttgca gcgagcgaat aatgcgtttg ccgacgcgac ccgcgccgca gacgatgtaa
      301 tgattctgta gtttttccat ttcttttatc ttccggcgtg aatccagcat ttctatcagt
      361 tccgcctgaa aaagcgtctg aacgagcgag gtcagggcat acaaaaccgt tccggcgccg
      421 acgagcataa aaaaaatcgc aaacactctg cccgtcgtgg atttcggcga caaatcgccg
      481 tagccgacgg tcgtcacggt ttcggtggcg agatagatgc tgtcgagcac cgaaaagcct
      541 tcgattagat gaaatccgaa cgcgccgatg gagatgacga cgaaaatcgc aatcaaagca
      601 tacaaaagct gcgagcgggc gattattgat attatcggaa atcgtttcat taacggttga
      661 aagtatagtt ttcaaagcgt caaacttcaa tatatatttt tttggaacac ggcttgcaca
      721 gaggcaacgt attaagcaaa aaagcttcga cttatgtgtg ggaatcatac aaaagtactt
      781 tattttgcaa caaaaaggag aacatcatga ccaatgtaaa gataattatg acggaaaatc
      841 ccaagtgctg cgcgccggac acatctttgc aggaagtggc gaaaatgatg gttgagattg
      901 attgcggctg tattccggtc gtggacgaaa actcaaaacc catcggtatg ataaccgacc
      961 gcgacatcac cgttcgaacg gttgccgaag gaaaaaatcc gctcgatttg acggcaggca
     1021 aggtaatgac cagcgatgtg tacacggtca cgccggagac cagcatcgaa gaatgctgcc
     1081 gtttgatgga aaaaaaacag gtgcggcgga ttgcggtcgt cgattcgcag ggcatctgct
     1141 gcggaatggt cgctcaggct gatgtggcga actacgcttc gcgcaaacag acggctgaag
     1201 tggtgcagga agtttcaaaa tcgcctgcgt tttcacagtc ggcgactaca taaagcgaat
     1261 ccggacagag aagacggaaa acgcggaagc gcggagcgat aaatgataaa ttcatcgttc
     1321 cgcgtttccg cgtttgcgct tccgcgtttc tttttgctgg acataacgcg ccgaaagttt
     1381 ataaaatttc aatggctctt tatccgacga gtcaaactct gccggcggat tcgacatcta
     1441 acccgaaaaa gttttccttc cgaaaataat taatggcaaa gcagatagaa gtaaaaagca
     1501 aa
//
LOCUS       DASWNE010000851         3108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_240436, whole genome shotgun sequence.
ACCESSION   DASWNE010000851 DASWNE010000000
VERSION     DASWNE010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240436"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1496
                     /locus_tag="VGB68_16575"
     CDS             <1..1496
                     /locus_tag="VGB68_16575"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0050910.1"
                     /translation="PSDFNLVAHNDNMRDTLLSKVQVTLKPGNIYHVAVDGFNDNGNI
                     AAGNFRIRFEKLSAPANDLIDAWNPDARLPMSTHGFTSGTNRNATKEPGEPNITNNVG
                     GKSVWYFWRAPDSRSMTVKLTCNPFVQECFDMQLAVFKGMNDKVASNDNYDNSTQTSS
                     VTFVAEEDVTYGFAVDGIKLPNGAVGEGNFFLDFYPTEYRYDTNFDATDRKADVSVFR
                     PDDGTWYTLQSSTNQISAVKFGVSGDVPVPADYDGDGVTDYTVVRNTPQGKYWYFLNS
                     SNNSFQAISYGLPDDKILAGDYDGDGKADMAAVRQTAQGLMWYIRPSSAPNNINAYPF
                     GLNTDQPVLGNFLKGFDTIGGLLATDLAVVRAEPDGKKTWHIQDITGFNYKQMQFGLA
                     SDVNVPADYDRDGYTDIAVWRPSNGTWYIFDESANKLLVRQWGMNGDIPLPAQFDDTG
                     APELVVYRPSTGVWWIYSNAGSGFTSSIKWGLSTDKPVSALTPLMNP"
     gene            complement(1635..>3108)
                     /locus_tag="VGB68_16580"
     CDS             complement(1635..>3108)
                     /locus_tag="VGB68_16580"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0050911.1"
                     /translation="ERRHIVGGAVCTQDDLIEGYKIDVGSSAHIMIHLTPVVRELELE
                     KFGLVYIDCDPFAFAPMADGKSAIYFWKDVDKTCESIARISPEDAERYKKFVAEWEKL
                     NEGVFEAFLKPPTAFNLARHMIFGTKSKNPADMVRKLMTSYGALVKQTFKHEGLRAAL
                     AWLAAQSGPPPTAAASGDFLGWHSMIHRSGVKRPRGGSGALTQAMAKCLAHHGGEILL
                     NAEVARIEVESGATRGIILANGERIEAARIVSNAHVHTTFLKLIGEENLPAGLAERIE
                     NVRIGNGFGMIVRCAVSELPDYSSAPSGGKPADCHRGLQLLCPSMDYLEKSYADYLNG
                     IPSRNPSALAMTFSAVDDTLAPAGKHTLFIWGQYFPYELSNGENWENIQEREADKLID
                     VVNAYAPNVKNSIIDRFVQSPLDLERRLGLLRGNVMHVEMDFDQMFLFRPLPEMSAYK
                     TPIKNLYLTGASTHPGGGVFAASGYNTAQVVLKDVKGGWF"
BASE COUNT          733 a          857 c          817 g          701 t
ORIGIN      
        1 ctccgagcga tttcaatctc gtcgcgcata acgacaatat gcgcgatact ttgcttagca
       61 aggttcaggt gacccttaaa ccgggaaaca tctatcacgt cgccgttgac ggatttaacg
      121 ataacggcaa catcgccgcc ggcaatttta gaatccggtt tgaaaagctg agcgccccgg
      181 ctaatgacct gatcgatgcc tggaatccgg acgcccggct gccgatgtcc acgcacgggt
      241 ttaccagcgg caccaaccgc aacgcgacta aagagccggg tgagcctaac attacgaata
      301 atgtcggcgg caaatcggtc tggtatttct ggcgcgcgcc ggactctcgt tcgatgacgg
      361 tgaagctgac ctgcaatcca ttcgtgcagg aatgttttga tatgcagctc gccgttttta
      421 agggtatgaa cgataaagtc gcttcgaatg ataattacga caacagcacg caaacatcga
      481 gcgttacgtt tgtcgccgaa gaagacgtaa cttacggttt cgccgttgac ggcatcaaac
      541 tgccgaacgg cgccgtcggc gaaggaaatt ttttcctgga cttttacccg accgaatatc
      601 gctacgacac caattttgat gcgaccgacc gaaaagccga cgtcagcgtt tttcgccccg
      661 acgacggcac ctggtataca ttgcagtctt ctaccaacca aatctcagcc gtcaaattcg
      721 gcgtcagcgg cgacgttccc gtgccggctg attatgacgg cgacggcgtg accgattaca
      781 cggtcgtcag aaacacgccg cagggcaaat actggtattt cctgaacagc tcgaacaaca
      841 gttttcaggc aatctcctac ggtttgccgg atgacaaaat actggcgggc gattacgacg
      901 gcgacggcaa agcggatatg gctgccgtgc gccaaaccgc gcagggttta atgtggtata
      961 tccgcccgag cagcgcgccg aataatatca acgcgtatcc tttcggatta aacacggacc
     1021 agcccgttct cggcaacttt ttgaaaggtt tcgatacgat cggcggattg ctggcgaccg
     1081 atttagcggt cgtccgcgcc gaaccggacg gaaagaaaac ctggcacatt caggacataa
     1141 cgggttttaa ttacaaacag atgcagttcg gtctggcgtc ggacgtcaat gttccggctg
     1201 attacgaccg cgacggttat acggacatcg cggtttggcg tccctcgaac ggaacctggt
     1261 atatcttcga cgaaagcgct aataaactgc tggtcagaca atggggaatg aacggagaca
     1321 ttccgctgcc cgcccagttt gacgacaccg gcgcgcccga gctcgtcgtt tatcgtccga
     1381 gcaccggcgt ctggtggata tattccaatg ccggttccgg gtttacttcc tctatcaaat
     1441 ggggattatc gaccgacaag cccgtttccg cattgactcc tttgatgaat ccttaatctg
     1501 aatcaatcaa tttcaaatta aaaaaaggga cgaggctgcg ctttcgttga gagcagcctc
     1561 gtcccttttt ttatcggcga gcaacgcaga gcatttaaaa attcactaaa tgatgccgaa
     1621 aagtatttaa aatattaaaa ccagccgcct ttcacgtctt tcaaaacgac ctgcgccgtg
     1681 ttataacccg aagcggcgaa cacgccgccg ccgggatgcg tcgaagcgcc ggtcagatag
     1741 agatttttta tcggcgtttt ataggcggac atttccggca gcgggcggaa aagaaacatc
     1801 tggtcgaaat ccatctcgac gtgcatcacg tttccgcgca gcagaccgag ccgccgctcc
     1861 aaatcgagcg gcgattggac gaatctgtca attatcgaat ttttgacgtt cggcgcgtag
     1921 gcgttgacca cgtcgattaa tttatcggct tcgcgctcct ggatgttttc ccaattttcg
     1981 ccgtttgata attcatacgg aaaatattgt ccccagatga aaagcgtgtg tttgccggcg
     2041 ggcgcaagcg tgtcgtcgac ggctgaaaaa gtcatcgcca gcgccgacgg atttcgtgac
     2101 ggaatgccgt tcagataatc ggcgtaggat ttttccagat aatccatcga agggcagaga
     2161 agctgtaaac cgcgatgaca atcggcgggc tttccgccgc tcggagccga tgaataatcg
     2221 ggcaattccg aaacggcgca gcgcacaatc atcccgaagc cgttgccgat gcggacgttt
     2281 tcgattcttt ccgccaagcc cgcgggcaaa ttttcctcgc cgattagttt gagaaaggtc
     2341 gtgtgaacgt gcgcgttgga aacgatgcga gccgcttcga tgcgctcgcc gtttgctaaa
     2401 attattccgc gcgtcgcgcc cgattcgact tcgattcgag cgacttccgc gtttaataaa
     2461 atctcgccgc cgtgatgcgc caggcatttc gccatcgcct gcgtcagcgc gcccgaaccg
     2521 ccgcgcggac gctttacgcc cgaccggtga atcatcgaat gccagccgag aaaatcgccc
     2581 gaagccgcgg cggtcggcgg cggaccggac tgcgccgcca gccaggcaag cgccgcgcgc
     2641 aagccttcgt gtttgaaagt ttgtttcacg agcgcgccgt aagaagtcat cagcttgcgc
     2701 accatatcgg cgggattttt cgatttcgtg ccgaaaatca tatgccgcgc cagattgaaa
     2761 gcggtcggcg gtttcaggaa agcttcaaaa acgccctcgt tcagtttttc ccattcggcg
     2821 acgaattttt tatagcgctc ggcgtcttcg ggcgagatgc gggcgattga ttcgcaggtt
     2881 ttatcgacgt ccttccagaa ataaatcgcg cttttcccgt ccgccatcgg cgcaaacgcg
     2941 aacgggtcgc agtcgatata aaccagaccg aatttttcca gctccaattc gcgaacgacc
     3001 ggcgtcagat gaatcataat atgcgcgctg gagccgacgt caatcttgta gccttcaatc
     3061 aaatcatcct gcgtgcaaac cgcgccgccg acgatatgac gccgctca
//
LOCUS       DASWNE010000852         3325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_521481, whole genome shotgun sequence.
ACCESSION   DASWNE010000852 DASWNE010000000
VERSION     DASWNE010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521481"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="VGB68_16585"
     CDS             complement(<1..711)
                     /locus_tag="VGB68_16585"
                     /inference="COORDINATES: protein motif:HMM:NF039894.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY0050912.1"
                     /translation="MYEFAVTPAITQLRRPGVLITEVTPESLAEELELAPNDRIVRVN
                     GRTVRDYLDFRFQTAGETELTLQVKKSGGETWELEIEREEGEDFGLMFEQIVPRQCAN
                     ECIFCFCKGNPEDARPSLFVRDEDIRLSFLYGNYTTLSSITEDELKRIVEQRLSPQYV
                     SVHATDLKTRAFLLGVDEKRADISDKLKRLLDNDIEIHAQVVLCPEINDGKILEKTVR
                     DLAAHYPKIVSTAIVPVAL"
     gene            840..1259
                     /locus_tag="VGB68_16590"
     CDS             840..1259
                     /locus_tag="VGB68_16590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0050913.1"
                     /translation="MSDEKLPAGNDSVSPKTKILLADDDTSIRRFLEIVLGRLNYDVT
                     AAEDGLAAMKLALSDDFDLVVADAVMPHLTGYDLCRMLKQNPKYKNVPLVILSGMENN
                     SENADRCIADAYLLKGENLKQQLAETLSSLLAQRIAA"
     gene            complement(1227..1634)
                     /locus_tag="VGB68_16595"
     CDS             complement(1227..1634)
                     /locus_tag="VGB68_16595"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEY0050914.1"
                     /translation="MSVRIKILAALYILVLAGIVVLADVKETQYLFRPVRRLPYGDKI
                     GHFLLMGMFSLVVNLILKARSVRVWKLSYLLGSLIVLAIVTIEEFSQIFIRGRSFDVA
                     DLAADWAGIIIFGEIARLACRKFLRGDSLRQKA"
     gene            complement(1725..2864)
                     /gene="ald"
                     /locus_tag="VGB68_16600"
     CDS             complement(1725..2864)
                     /gene="ald"
                     /locus_tag="VGB68_16600"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEY0050915.1"
                     /translation="MKIGLPKEIKDNEYRVGLTPAGVNALVNAGHEVYVENRAGEGSG
                     FANELYEKAGATVLQTADDVWQTGEMIVKVKEPIAPEYPRMRENQLLFTYLHLAPEFE
                     LTKQLLERKVTGVAYETITDRRGTLPLLTPMSEVAGRMAVQVGATYLEKMNGGRGILL
                     GGVPGVPAANVVIIGGGVVGTEAAKMAVGLGAKVTIIDINLDRLRQLDDIFLSKVQTL
                     ASSRYAIHEAISHADLVIGGVLIPGAAAPKLVTRDMLKDIPNGAVLVDVAVDQGGCFE
                     TTHATTHSNPTFYEEGVLHYCVANMPGAVPRTSTFALTNATLPYALALANKGFEKAIQ
                     EDAGLAEGVNTYAGKLTYEAVAESQNLEYTALNSLIDGIAAKATN"
     gene            complement(3130..>3325)
                     /locus_tag="VGB68_16605"
     CDS             complement(3130..>3325)
                     /locus_tag="VGB68_16605"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0050916.1"
                     /translation="TGICYVNSATIGAEVHLPFGGTKGTGNGHREAGTQVLDIFSEWK
                     SLYIDYSGKLQKAQIDLVEI"
BASE COUNT          769 a          898 c          823 g          835 t
ORIGIN      
        1 cagggcgacc gggacaatcg ccgtcgagac gattttcgga taatgcgccg ccaaatcgcg
       61 cacggttttt tccagaattt ttccgtcgtt gatttccggg cagagaacga cctgcgcgtg
      121 gatttcgatg tcgttgtcta aaaggcgttt taatttatcg gaaatatcgg cgcgtttttc
      181 gtcgacgccg agcaggaagg ctcgggtttt taaatccgtc gcgtgaaccg agacgtattg
      241 cggcgacagg cgctgctcga cgatgcgctt cagctcgtct tcggttatcg aagaaagcgt
      301 cgtgtaattt ccatacaaaa acgaaaggcg gatgtcttcg tcgcggacga aaagcgacgg
      361 gcgcgcgtcc tcggggttgc ctttgcagaa acaaaaaatg cattcgttgg cgcactggcg
      421 cggcacgatt tgctcgaaca tcaagccgaa atcttcgcct tcttcgcgct cgatttccag
      481 ttcccacgtt tcgccgccgg attttttaac ttgcagcgtc agctcggtct cgcccgccgt
      541 ctgaaagcga aagtccagat aatcgcgcac cgttcgcccg ttgacgcgga cgattcggtc
      601 attcggcgcg agctccaatt cttccgccag gctttcgggc gtgacttccg taattaaaac
      661 tccggggcgg cgcagttgcg tgattgcagg tgtaacggca aattcgtaca tttggaaaat
      721 tcaataattt gaacaactta gagttatttc attgacaaac ggttgccagt tcttttattc
      781 taaaaaatgg ttttgcattt gccaaaatcg caaaaccagg ttcaacaaac ggagaattta
      841 tgtctgatga aaaattgccg gcaggcaatg attcagtttc tcccaaaacg aaaatcctgc
      901 tcgctgacga tgatacttcc attcgacgct ttctcgaaat cgttttaggg cgattaaatt
      961 acgatgtcac ggctgccgaa gacggcttgg cggcaatgaa actggcgctt tccgacgatt
     1021 tcgatttggt cgtcgcggat gcggttatgc cgcatttgac cggctacgat ttgtgccgta
     1081 tgttaaagca aaatccgaag tataaaaacg tgccgctcgt tattctaagc ggcatggaaa
     1141 acaattcgga aaacgccgac cgctgtatcg ccgatgccta tctgctgaaa ggcgaaaatc
     1201 tcaaacaaca gcttgccgaa acgctctcaa gccttctggc gcaaagaatc gccgcgtaaa
     1261 aatttgcggc acgccagccg cgcaatctcg ccgaaaataa taattcccgc ccaatccgcc
     1321 gccaaatccg ctacgtcaaa cgagcgcccg cgaataaaga tttgcgaaaa ttcttcaatc
     1381 gtgacgattg ccagaactat caggctgccg agcaggtaac ttaatttcca gacgcgaacg
     1441 cttctcgcct tcaaaatcag attaacgacc agcgaaaaca ttcccatcag cagaaaatgt
     1501 ccgattttat cgccgtacgg aagccgccga accggtcgga aaagatactg cgtttccttt
     1561 acgtcggcta aaacgacgat gcccgcgaga accaaaatat aaagcgcggc taaaattttt
     1621 attctcacgg acattaattt agtggatagt ggatagttga tagttgatag ttgatagtta
     1681 atagttgaaa atcaaactat cagttatcaa ctatcagttt tcagttaatt tgtggctttc
     1741 gccgcgattc cgtcaatcag gctgttgagc gcggtatatt cgagattttg tgattcggcg
     1801 acggcttcgt aggtcagctt tccggcgtaa gtattgacgc cttcggctaa acccgcgtct
     1861 tcctgaatcg ctttttcaaa gcctttgttt gccagcgcca gcgcatacgg caaagtggcg
     1921 ttggtcaggg caaaggtcga cgtgcgggga accgcgcccg gcatattcgc cacgcagtaa
     1981 tgcaggacgc cttcttcgta aaacgtcggg ttcgagtgcg tcgtcgcgtg cgtcgtttca
     2041 aaacagccgc cctggtcaac cgcgacatcg acgagaaccg cgccgttcgg gatgtctttc
     2101 agcatatcgc gcgtgaccag tttcggcgcc gccgcgcccg gaatcagaac gccgccgata
     2161 accaaatcgg cgtgtgaaat cgcttcgtga atcgcgtagc gcgaagaggc gagcgtctgc
     2221 actttcgata aaaagatgtc gtccagctgg cgcagccggt cgagattgat gtcgataatc
     2281 gtcactttcg cgcccaagcc gaccgccatt ttcgccgctt ccgtgccgac cacgccgccg
     2341 ccgataatga cgacgtttgc cgccggaacg cccggaacgc cgccgagcag aattccgcgc
     2401 ccgccgttca ttttttccag ataagtcgcg ccgacctgca ccgccattct gcccgccact
     2461 tccgacatcg gcgtcaaaag cggcagagtc ccgcgccggt cggtgattgt ttcgtaagcg
     2521 acgcccgtca ccttgcgttc cagaagctgt ttggtcagtt caaattccgg cgccagatgc
     2581 agataggtga aaagcagctg gttttcacgc attcgcgggt attccggcgc aatcggctct
     2641 ttgaccttca caatcatctc gcccgtctgc cagacgtcgt cagccgtctg caaaactgtc
     2701 gcgcccgctt tttcgtaaag ctcgttggca aaacccgagc cttcgccggc gcggttttcg
     2761 acgtaaactt cgtgtcccgc gttaaccagc gcgttaacgc ccgccggcgt cagcccgacg
     2821 cgatattcgt tatccttaat ttctttcggc aatccgattt tcatttatag ctctccaaac
     2881 ttgtattttt gataattaaa aacgtaactt tcaattttac gtggttttcg ccgaattgtt
     2941 aaagcgaact gttgtaattc cgatactttt tcaaatcttc ttcggtcgcc gcatttaaac
     3001 tgacctctgc gccgccgcct tccgccggtg cggttttcag tggcttaact ctctttatct
     3061 tccaaaaaac aaaatcggct gtaagtcgaa cagttttcga cttacagccc gcgtggcttt
     3121 tgtcaaaaat taaatttcga ctaaatcaat ctgcgctttt tgcagcttgc ccgaatagtc
     3181 gatgtagagc gatttccact cggagaaaat atcgagaacc tgcgttccgg cttcgcggtg
     3241 cccgttgccg gtgcctttcg tgccgccgaa cggcaggtgg acttcagcgc cgatggtcgc
     3301 cgaattgacg tagcagattc cggtg
//
LOCUS       DASWNE010000853         1566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_28511, whole genome shotgun sequence.
ACCESSION   DASWNE010000853 DASWNE010000000
VERSION     DASWNE010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28511"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1233
                     /locus_tag="VGB68_16610"
     CDS             <1..1233
                     /locus_tag="VGB68_16610"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF006006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY0050917.1"
                     /translation="TADKMKTTAGSLALLDAPTPRADAFLVQRLRQAGAVIIGKTNLS
                     EWANFRSTKSSSGWSARGGQTRNPYILDRNACGSSSGSGVAIAANLAALAVGTETDGS
                     VICPAATCGIVGIKPTLGLVSRSGVIPIAHSQDTAGPMTRTVTDGAILLSAMVGYDAS
                     DSITSQANKGAKDYTQFLQKDGLRGMRIGVGRQFFERSAKIDAVIEPHLQTLKDGGAT
                     LIDVQFPNLNKFGDAEFEVLLYEFKADLNKYLAQRNSPYKTLADLIKFNEDNREREMP
                     YFGQEIFLMAEKKGDLNTREYRMALQKSKLLTQAQGLDMVITQNKLDAVVAPSGGAAW
                     MIDLVNGDCGVFESGSMAAVAGYPIVTVPAGYVQGLPAGISFFGRAFSEPALIKIAYA
                     FEQATTARVAPKFLPTYQ"
BASE COUNT          416 a          386 c          444 g          320 t
ORIGIN      
        1 accgccgaca aaatgaaaac gaccgccgga agcttggcgc ttttagacgc gccgacgccg
       61 cgcgccgacg cttttctggt tcagcgattg cgccaggcgg gcgcggtcat tatcggcaaa
      121 accaacttga gcgaatgggc gaattttcgc tcgaccaaat cttcgagcgg ctggtcggcg
      181 cgcggcggac aaacccgaaa tccgtatatt ctggacagaa acgcctgcgg ctcgagctcc
      241 ggctcgggcg tggcaatcgc ggcaaatctg gcggcgctcg cggtcggcac ggaaaccgac
      301 ggctcggtta tctgtccggc ggcgacgtgc ggcatcgtcg gcatcaaacc gacgctcgga
      361 ctggtctcgc gttcgggcgt gattccgatt gctcattcgc aggatacggc gggaccgatg
      421 acgcgcaccg tcacggacgg cgcgattctt ttgagcgcga tggtcggcta cgacgcgagc
      481 gattcgataa cttcgcaggc aaacaaaggc gcgaaagatt acacgcagtt tctgcaaaaa
      541 gacggcttgc gcggaatgcg aatcggcgtc gggcggcagt ttttcgagcg cagcgcgaaa
      601 atagacgccg ttatcgagcc gcatctgcaa acgctgaaag acggcggcgc gactttgatt
      661 gacgttcagt ttccgaatct gaataaattc ggcgacgcgg aattcgaggt tttgctttac
      721 gagttcaaag cggatttgaa caaatatctg gcgcagagaa attcgccgta taaaacgctc
      781 gccgatttaa tcaaattcaa cgaggacaac cgcgagcgcg agatgcccta tttcggtcag
      841 gaaatttttc tgatggcgga aaagaaaggc gatttaaaca cgcgcgaata tcgaatggct
      901 ttgcagaaat caaagctttt gacgcaggcg cagggcttgg atatggtcat tacgcaaaat
      961 aagctggacg cggtcgtcgc gccttcgggc ggcgcggcgt ggatgattga tttggtcaac
     1021 ggcgattgcg gcgtctttga aagcggctcg atggcggcgg tcgccggtta tccgattgtg
     1081 accgttccgg ctggttatgt gcagggactt cccgccggaa tttcattttt cggacgcgcc
     1141 ttttccgaac cggcgctgat aaaaatcgct tacgctttcg agcaggcgac cacagcgcgc
     1201 gtcgcgccga agtttttacc gacgtatcaa tagtaaaaag aaggcagtcg gcagaaagcg
     1261 gaaagcagta atgaattcga atgataatcg tctctaacag atgaacaatc acctctaatg
     1321 agtgataatc gtctacaacg aatgtgcaat cgcctaaaac gagcgtgcaa tcgcctaaaa
     1381 cggactgaca atcacctaaa acgggctgac aattagctaa aacggatgaa taactagcta
     1441 aaacgagtga acaattagct aaaacgagtg ataatcgtct aaaacggata tgcaatcacc
     1501 taaaacggat gaaaatcgtt taaaacgagt gaaaattgac aaaaacgagc ggaaatcgtg
     1561 aaaaac
//
LOCUS       DASWNE010000854         4249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_521584, whole genome shotgun sequence.
ACCESSION   DASWNE010000854 DASWNE010000000
VERSION     DASWNE010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521584"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..751)
                     /locus_tag="VGB68_16615"
     CDS             complement(146..751)
                     /locus_tag="VGB68_16615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050918.1"
                     /translation="MKEDYLWDKTGEDPDIEQLENALQVFRYKETAPFELPAAKVLPF
                     RKEETAPPRRIFSFTLAAAACLIFGIIALGVWLQVLNNNAEVQTDLTEKAKPEIAVPP
                     PPPVADPDGLRVNNVENAKRFPARKIAAIRGKVPVNIRRKQTKAPNARARKPADRLTK
                     EEQYAYDQLMLALSITSSKFKLVKDKVEGTEEKNALLGDGQ"
     gene            complement(755..1333)
                     /locus_tag="VGB68_16620"
     CDS             complement(755..1333)
                     /locus_tag="VGB68_16620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0050919.1"
                     /translation="MTGNLLFFASAEATAAADATATAAKVKAEARLVERVRAGDREAF
                     GELYKMFAPLVHGIVLARVPRDEADDIAQNVFLSAYKNLHNLRDYNAFGAWLAMIARN
                     YSNEFYRHEKPTEELSEELAQTDAPHAEAREILSAIRSLPDAYRETLVLRLVDGMTGP
                     EIAEQTGLTPESVRVNLHRGMKLLRRKLGVGG"
     gene            complement(1600..2403)
                     /locus_tag="VGB68_16625"
     CDS             complement(1600..2403)
                     /locus_tag="VGB68_16625"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HEY0050920.1"
                     /translation="MFTDNQFTLSQIQNWMQSALVQPDSQTEPLKPEDFINESSRLTA
                     RRHLNIYRQSYIARLRECMKNQFSALAFALGEELFQMFADQYLDACPSASYTLNDLGK
                     KFPDFLVETRPDAAEQEKEEWVDFIIELARFEYELSVIFDERSEEKFEPANDATPDEA
                     LKLAPLFYLFHHRHPVSRYYLDVTQKKAPELPFEEETFCAVVRNDYRLALSELKPAQY
                     YFLERLSKGESVARAKEYFINTYNFDAGKLDAVWKEWRQNFIASGFFRA"
     gene            complement(2420..3382)
                     /locus_tag="VGB68_16630"
     CDS             complement(2420..3382)
                     /locus_tag="VGB68_16630"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HEY0050921.1"
                     /translation="MNKEVFRECLRRLPEPALGLGLRSRHFNYILENNPAVDWFEVIS
                     ENFMDSGGRPRSVLRRIAERYPVVMHGVSLSIGSADALNVEYLKKLKALAAEIEPLWI
                     SDHLCWTGVNGLNTHDLLPLPLTEESLKHVCRRVNQIQDFLQRPVIFENPSTYLTFKQ
                     STVPEWDFLRRMTEETGCGLLLDVNNVYVSGFNNDFDPAHYIRQLPPESIVQIHVAGH
                     RHCGDYIIDTHDDYVTRDVWELFALAWQLTGGAPTLLEWDSNIPDFEGYHAELLKAKD
                     FMSAFAADRDIEKQTEYGEAGVSNPVDFLVADVQYEFNRQPESF"
     gene            complement(3375..4079)
                     /locus_tag="VGB68_16635"
     CDS             complement(3375..4079)
                     /locus_tag="VGB68_16635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050922.1"
                     /translation="MDNKELPTIENLCGGGGGCSTPSPAPTPAPSATTVTAPPLELHI
                     CMGLNACKGHDRYGTNDCAGKGWCATAQAHTCHTLNNCRGQGGCGLYGDGEEQNYPGA
                     NDCAWQGSCAVPVQAERFSTLGPNKGKSVWVLARRLFEERMRKANRTFGASPYKCGPP
                     ENWLTEIMGEYDSCGNSGDKYCTFGFNDPAKDAQELCQKSQPHLCGSSASEANHCSSP
                     SEANRGGAAEKNSSDE"
BASE COUNT          939 a         1160 c         1100 g         1050 t
ORIGIN      
        1 agcactcaaa tccatcgcaa agtaacgcag gagaagccga aatcgcttct ctgcaatatg
       61 ggaacgccgg taataacggt ttaacccaga catatcagaa gtttagcact accttttggt
      121 ctctaaactt ctctagaccc ataaattatt gtccgtcgcc gagaagggcg tttttttctt
      181 ccgttccttc gaccttgtct tttactaatt tgaacttcga gctcgtaatc gaaagcgcga
      241 gcatcagctg gtcgtaagcg tattgttcct ctttcgtcag ccggtcagcc ggttttctcg
      301 cccttgcgtt cggcgctttt gtttgcttcc ggcgaatatt taccggaact tttccgcgaa
      361 tcgcggcaat tttacgcgcg ggaaatcgct ttgcattttc gacgttgtta actcgtaacc
      421 cgtccgggtc ggcaaccggc ggcggcggcg gcactgcaat ttcaggctta gctttttccg
      481 tcaaatccgt ctgaacttca gcgttattat ttaaaacctg aagccagacg cccagagcga
      541 ttatgccgaa tatcagacag gctgccgcgg caagagtaaa cgaaaaaatc cggcgcggcg
      601 gcgcggtctc ttcttttctg aaaggcagaa cttttgcggc gggcagctca aacggcgcgg
      661 tttctttata acgaaacact tgcagcgcgt tttccagttg ctcgatgtcg gggtcttcgc
      721 ccgttttgtc ccacagataa tcttctttca ttatttaccc tccgacgccc agcttccggc
      781 gcaaaagctt catccctcgg tgcagattca cgcgcaccga ttccggcgtc aacccggtct
      841 gttcggcgat ttccggtccc gtcattccat cgaccagacg caaaaccaga gtctctctat
      901 acgcgtcggg caaagaccgg atagccgata aaatctcgcg cgcttccgcg tgcggcgcgt
      961 ccgtttgagc cagttcttcg gataattcct cggtcggttt ttcgtgacgg taaaactcgt
     1021 ttgaatagtt gcgggcaatc atcgccagcc acgcgccgaa cgcattgtaa tcgcgcaggt
     1081 tgtgcagatt tttataagcc gagagaaaaa cgttttgagc aatatcatcg gcttcgtcgc
     1141 gcggcacgcg agccaggacg atgccgtgaa ccagcggcgc gaacatcttg taaagctcgc
     1201 cgaatgcttc gcggtcgccg gcgcgcacgc gttcgaccag ccgcgcttcg gctttgacct
     1261 tcgccgccgt cgcggtcgca tccgccgccg ccgtcgcttc ggcgctcgca aaaaaaagta
     1321 aattgccggt catttcgctt tcaggcatcc gtctaaattg tgtcgggcga tgaaacaaat
     1381 aaaaatgaat atgaaaaaat tattaaaccg cttcatcgcc cgacggcaaa cggcttttaa
     1441 tatcctgccg attatataga cgagtcaaga ccggaaagtg ttaacagggt tttcgagaaa
     1501 attttcggcg aagtcgtgaa acaaaaaacg tgcccgcgaa cggaaatata ggggcggacg
     1561 cgccggcgcg tgcgccccta tatttccgtt acccggattt caggcgcgga aaaagcctga
     1621 cgcgataaag ttctgtctcc attctttcca gacggcgtcc agttttccgg cgtcgaaatt
     1681 gtaagtatta ataaaatatt cttttgcccg cgcgaccgat tcgcctttcg acagtctttc
     1741 gagaaaataa tattgcgccg gtttcagctc cgataaagcg agccggtaat cgtttcgcac
     1801 gacggcgcaa aaggtttctt cctcgaaagg aagctccggc gcttttttct gcgtcacgtc
     1861 cagataatat ctcgaaaccg ggtggcgatg atgaaaaaga taaaacaaag gcgcgagctt
     1921 taaggcttcg tcgggcgtcg cgtcgtttgc cggttcgaat ttttcttccg aacgctcgtc
     1981 aaaaatcacc gacaactcgt attcgaagcg cgccagctcg attataaaat ccacccattc
     2041 ctctttttcc tgctcggcgg cgtcgggtct ggtctcgacg agaaaatcgg gaaacttttt
     2101 gccgaggtcg tttaaggtat agctcgcgga cgggcaggcg tcgagatact ggtcggcgaa
     2161 catttgaaat aattcttcgc ccagcgcaaa ggcgagcgcg ctgaactggt ttttcataca
     2221 ttcgcgcagg cgggcgatat agctctgccg gtagatattc aaatgccgcc gcgccgtcag
     2281 acggctcgat tcgttaataa aatcttccgg cttgagcggc tcggtttgag aatccggctg
     2341 gacgagcgcc gactgcatcc agttttgtat ttgtgaaagc gtaaattgat tgtccgtaaa
     2401 cattattaat gattgatgct taaaaagatt cgggttgccg gttgaattcg tactgcacgt
     2461 ccgcgaccag aaaatcgacc ggattcgaga cgcccgcttc gccatattcg gtttgtttct
     2521 ctatatcgcg gtcggcggca aaggcgctca taaaatcttt tgcctttaac aattcggcgt
     2581 gatagccttc aaagtccgga atattgctgt cccattccag cagagtcggc gcgccgcccg
     2641 tcagttgcca cgccaatgcg aaaagctccc agacatcccg cgttacataa tcgtcgtgcg
     2701 tgtcgataat gtaatcgccg caatgacgat gcccggcgac gtgtatctgg acgatgctct
     2761 cgggcggcag ttgccgtata taatgcgccg ggtcgaaatc gttattgaat cccgaaacat
     2821 agacgttatt gacgtccagc aacaagccgc agcctgtctc ttccgtcata cggcgcagaa
     2881 aatcccattc gggaacggtc gattgtttga aagtcaggta agtgctcgga ttttcaaaaa
     2941 taacggggcg ttgcagaaaa tcctgtattt gattaacccg ccggcagacg tgttttaagg
     3001 attcttcggt cagcggcaga ggcagtaaat cgtgcgtgtt caagccgttg acgcccgtcc
     3061 agcataaatg gtcgctaatc cagagcggct cgatttccgc agccagagcc ttcagttttt
     3121 tcaggtattc gacatttaaa gcgtcggcgc tgccgataga aagcgaaacc ccgtgcatga
     3181 ccacggggta gcgttcggca atccggcgca ggactgagcg cggtctgccg ccggaatcca
     3241 taaaattttc ggaaatcacc tcaaaccaat cgacagccgg attattttcc agaatataat
     3301 taaagtgcct gcttcttaag cccagcccga gcgcgggctc gggcagccgc cgaagacact
     3361 cgcgaaacac ttctttattc atcggatgag tttttttccg ccgcgccgcc gcggtttgcc
     3421 tcgctcggag agctgcaatg attcgcctcg ctcgccgaag agccgcaaag gtgcggctgg
     3481 cttttttgac aaagctcctg cgcatctttg gcggggtcgt taaatccgaa cgtgcagtat
     3541 ttatcgccgc tgttgccgca cgaatcgtat tcacccataa tctcggtcag ccagttttcc
     3601 ggcggtccgc atttatacgg cgacgctccg aaagtgcggt tggctttgcg cattctctct
     3661 tcaaacaatc tgcgcgccag cacccagacg ctttttcctt tattcggtcc cagcgtgctg
     3721 aagcgctcag cctgcaccgg aacggcgcag cttccctgcc aggcgcagtc gttagcgccg
     3781 ggataatttt gttcttcgcc gtcgccgtat aaaccgcagc cgccctgccc gcggcagttg
     3841 ttcaaagtat gacaggtgtg cgcctgtgcc gtcgcgcacc agccctttcc cgcgcagtcg
     3901 ttcgtgccgt agcggtcgtg acccttgcag gcgtttaagc ccatacagat gtgcagctcc
     3961 agaggcggcg cggttactgt agttgcgctt ggagccggcg tcggcgcggg cgacggcgtc
     4021 gaacagccgc ctccgccgcc gcagagattt tctattgttg gaagctcttt attgtccata
     4081 gtcgtaattt ttcgatgtca gataatcagt tcgaattgat gacgaagcgg cgaaaacgcg
     4141 ccgcgtcagt taagcggaac gtccggcaaa tcctgaaatc tttgcgccag ataggaaatg
     4201 ttcggataca gggcgacggc gttgccgtaa agctcgagca gctgcgctt
//
LOCUS       DASWNE010000855         1538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_624517, whole genome shotgun sequence.
ACCESSION   DASWNE010000855 DASWNE010000000
VERSION     DASWNE010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624517"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1538
                     /locus_tag="VGB68_16640"
     CDS             <1..>1538
                     /locus_tag="VGB68_16640"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0050923.1"
                     /translation="NTASLVEQRKDGTEKDSKDSWFYSKVEGFYMWLLKLSMRHRWAI
                     VGICILVVVSIYPLFKLVGMAFLPDEDESAFQVSVRGPQGTSLAATQSILDRISRDIR
                     QQIPGVQNTVVLAGGFGGGGGSGNAGSINVALAPVSERKFSQAELINRTRGLVRRYNT
                     KDYRVNVSGSSSIGSSLGLGRGGSGVGFYIAGPDIQKLDEYSNALVEKLRADDETFRD
                     ADRSLELGSPEIRVLIDRAKAADLGVEAGDVATAINILSAGQRVSTFAEGSDQYDVVV
                     QADEQFRRSRNNLQYFTVASTNGTGTVGLEKLVRLEEGLSPASINRLNRQRQVTISAS
                     LPPNASESDALAKLQQHAASLNMPQDYRTGVTGQSQELQRAYDAFLLAFLLSFVFMYL
                     ILAAQFESFIHPITILLTLPLSIPFALLSTLIAGQTLNIFSALGILLLFGIVKKNAIL
                     QIDHTNGLRAKGMNRYDAIIQANRDRLRPILMTTIALVAGMIPLVVSSGAGAGTNRSI
                     GVLV"
BASE COUNT          306 a          428 c          447 g          357 t
ORIGIN      
        1 cgaataccgc gtccttagtc gaacagcgga aagacggaac cgaaaaagac agcaaggatt
       61 cgtggtttta cagcaaagtc gaaggctttt atatgtggct cctgaagctt tcgatgcggc
      121 atcgctgggc gatagtcggg atttgtatcc tggtggtcgt ttcgatttac ccgctcttta
      181 aattagtcgg gatggctttt ctgcccgacg aagacgaatc ggcttttcag gtttccgtcc
      241 gcggtccgca agggacgagc ctcgccgcga cgcagagcat tctcgaccgc atctcgcgcg
      301 acattcgcca gcagattccg ggcgtgcaga acacggtcgt gctcgccggc ggtttcggcg
      361 gcggcggcgg ttcgggcaac gcgggttcga ttaacgtggc tctcgcgccg gtcagcgagc
      421 gcaaattttc gcaagccgaa ttgattaacc ggacgcgcgg attggtcagg cgttacaaca
      481 cgaaagatta tcgcgtcaac gtttccggct cgtcgtccat cggttcgagt ctcggcttgg
      541 gacgcggcgg ttcgggtgtc ggtttttaca tcgccggacc cgacattcaa aagctcgacg
      601 aatattcgaa cgcgctggtc gaaaaactgc gcgccgacga tgaaactttc cgcgacgccg
      661 accgctcgct ggagctcggc agcccggaaa ttcgagtttt aatcgaccgc gcgaaagcgg
      721 cggatttggg cgtcgaagcc ggcgacgtgg cgacggcgat aaatattttg tcggctggac
      781 agcgcgtttc cacttttgcc gaaggctcag accaatacga cgtcgtcgtg caggcggacg
      841 agcaatttcg ccgctcgcgc aataatttgc agtatttcac cgtcgcttcg accaacggta
      901 cgggaaccgt cggtttggaa aaactcgtgc ggctcgaaga aggtttaagc ccggcttcga
      961 ttaaccgtct gaatcgccag agacaagtta cgatttccgc gagcctgccg ccgaacgctt
     1021 cggaatcgga tgcgctggcg aagcttcaac agcacgccgc ttcgctgaat atgccgcagg
     1081 actatcgcac cggcgtgacc ggacaatcgc aggaattgca gcgcgcttac gacgcgtttc
     1141 tgctcgcgtt tctgctttcg ttcgtcttta tgtatctgat tctggcggcg cagttcgaat
     1201 cgtttattca cccgattacg attcttctga ctttgccgct ttcgattccg tttgcgctgc
     1261 tctcgactct gattgccgga cagacgctga atattttctc ggcgctcggc attctgctcc
     1321 tgttcggaat cgtcaagaaa aacgcgattt tgcagattga ccacacgaac ggcttgcggg
     1381 caaaaggaat gaatcgttac gacgccatta ttcaggcgaa ccgcgaccgc ttgcgaccga
     1441 ttctgatgac gacgatcgcg ctggtcgccg gtatgattcc gctcgtcgtt tcgtcgggcg
     1501 cgggcgcggg aacaaaccgc tccatcggcg ttctggtc
//
LOCUS       DASWNE010000856         9788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_132809, whole genome shotgun sequence.
ACCESSION   DASWNE010000856 DASWNE010000000
VERSION     DASWNE010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9788
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132809"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VGB68_16645"
     CDS             <1..665
                     /locus_tag="VGB68_16645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050924.1"
                     /translation="AERKPVEACNFDLMEKLETFYSAAPDKFRDAEADSQFLKELKTC
                     PREQTINSIAALQKARREDGEIQAKTAYLLIKLDRDKKVNQKALFSVFPAYRDKTGGR
                     FRRDHILDLIGDVVINEDTGEKRFLADAFDLAPLVNAATAEMLSGILAGEFEKNPEIF
                     LLSLKNKPKAVKREVYSFISYQLPKDVLSKHLSSIPETSDAYRTGKEMSEFAENRKRV
                     SG"
     gene            735..3101
                     /locus_tag="VGB68_16650"
     CDS             735..3101
                     /locus_tag="VGB68_16650"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEY0050925.1"
                     /translation="MFKNSLFSAPVSLCALILFFAFSAFAQTKIPEPKDVLGFTPGDD
                     RKLASWNQTVDYFQKLDAASSRVQFQEIGRTTLDRPFVYAVISAPENLKNLEKYKAIN
                     AKLADPRRIMRSRASPAGIERQLLKDGKTIVLITCGIHSTEVGSTLSSMLIAHRLASA
                     GEPEIQKILQNTIILLVPSLNPDGVDIVKNWYDKTLGTPFEGTSPPELYHRYVGHDNN
                     RDWYAFTQVETQLTVDKIHSEWHPQIVHDIHQQGERGSRLFLPPYMQPVEPNVPQEIV
                     EGYTELGNFMADDLRAKGFQGITTNSTYDAWTPARAYSHYHAGVRILSETASAKLATP
                     VSVKFEDLRNGLGYDVRKESTNFSPVWLGGEWKLRDITNYMTAATFSLLNHAADNRAA
                     WLGRFYAVGKEAVRPRRAGELFAYVLPYPKSAESESGSEEYFRREELLGIFRRAGVET
                     KFAVNLRINGEKYPAGTVVIPIAQPYGNFAKALLEKQVYPNLRDEKGNPVAPYDVTAH
                     TLSLLMGVEAKPIYKSFAYKVTKNGLEQGRGCGCGGGSSEPGYLIYQSKVASMDEGWT
                     RWVTENAERFLNRCCFAGGSVTDREIKSQTFKSNYIIFPDQSSNQILNGYAKGSMPDE
                     YTGGVGAEGVKNLRRFVEDGGTLVFLNRASDFAIKELGAPVRDVTAGLQRRDFYIPGS
                     ILKTEIDTAHKIAAEMPKESIAWFENSPAFEIAGDAANVEIVARYPEKAENILLSGWA
                     LGAEKIAGKAALVSINIGKGKIILFGFRPQYRGQSLATFPLFFNAIAQ"
     gene            3163..3606
                     /locus_tag="VGB68_16655"
     CDS             3163..3606
                     /locus_tag="VGB68_16655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050926.1"
                     /translation="MFLFDDIDFVPAQSGELSRLWYFLPIGYLTTILIETPILLIGLS
                     PKLSLKQRFACGVWLTACTYPVVILVLPALLLDGSDRSRLVYLAVAETFAPAAECFFF
                     WLAFRGKNLFEQKDWIRSFAAIVVANLASFGIGEILNAAAWFGLF"
     gene            3690..4337
                     /locus_tag="VGB68_16660"
     CDS             3690..4337
                     /locus_tag="VGB68_16660"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY0050927.1"
                     /translation="MDLRVTEIFLSIQGESTHAGRPCAFVRQTGCPMRCRWCDSEYTF
                     TGGERLSFDEIFGRLKDFSCRLVEVTGGEPLAQKNVFPFIERLCDAGYEVLIETGGFL
                     STEKIDARAKIILDVKCPASGEAERNHWANLERLRAASDEIKFVIADLSDWEFARRII
                     EKYDLENRARSVLISPVFGVENLPEIAETVSRSGLKVRLNLQLHKYIWGADVHGV"
     gene            4341..4586
                     /locus_tag="VGB68_16665"
     CDS             4341..4586
                     /locus_tag="VGB68_16665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050928.1"
                     /translation="MTHYEKLSTMFFRILGAFLLLVGIIIAVTALITIPVNGLFPPVF
                     FIIFYSIPLMVLGILFFSASRKPAKWVCFDFDKINER"
     gene            4576..5256
                     /gene="queC"
                     /locus_tag="VGB68_16670"
     CDS             4576..5256
                     /gene="queC"
                     /locus_tag="VGB68_16670"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEY0050929.1"
                     /translation="MNDKKTAICLVSGGMDSCVTSAIAKSENEEIALLHVSYGQRTER
                     RERKAFQEIADFYGATRRLAVSIEYLARIGGSSLTDESIEIAEANLEASEIPTSYVPF
                     RNANMLSIATSWAEVAGANRIYIGAVAEDSSGYPDCRPEFYEAFEKAIDAGTKPETRI
                     EIRTPIIHLSKAEIVRKGLELNAPLHLSWSCYRNEDLACGTCDSCALRLRGFKLAGVK
                     DPIAYENY"
     gene            complement(5250..5771)
                     /locus_tag="VGB68_16675"
     CDS             complement(5250..5771)
                     /locus_tag="VGB68_16675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050930.1"
                     /translation="MKYAGFLSFLIFGFLFSANCERAKVERNTAEIETVTRQSENFSQ
                     KAETPEEKAVRLAEEFVRQNGYTDAAADENNLSRETVEFYEDPDDLLKQRRNSLEPKA
                     YGISPGARGNKKGWTVVFRYSGRFRDNLEAKDKSTGNLEKGGRAVTMNENFQNLLVEH
                     KDFRLEKIEKKLQ"
     gene            complement(5926..6618)
                     /locus_tag="VGB68_16680"
     CDS             complement(5926..6618)
                     /locus_tag="VGB68_16680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050931.1"
                     /translation="MESLNVKKIFLYLMICSIALSALMGIWAILSGDVEYRVLGTTLT
                     IVGTSILGLACGAFLENPRSQKFPLIAIPVVGIILSVLSALITIWLIWMKTNWSDEVL
                     FKTLAVSSIFAFALSHLSLLSIAQLSRRFRWSLITAFVVIFALASIISFIIVFEPPSE
                     SGIVLRLIGVLAVIDAALTVMIPIFHRLSRTEFVDIPSVAEIEAKIAGLKAQITRLEK
                     QKEDVLRAEQPA"
     gene            complement(6669..6914)
                     /locus_tag="VGB68_16685"
     CDS             complement(6669..6914)
                     /locus_tag="VGB68_16685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050932.1"
                     /translation="MKTISKLLIIIGTFIVFVADSYALWNFFGSFNAMKSSEASGIGA
                     FATAIDSAYQGVLLSFVGIFFLFIGCILLLIPTRAKN"
     gene            complement(6976..8259)
                     /locus_tag="VGB68_16690"
     CDS             complement(6976..8259)
                     /locus_tag="VGB68_16690"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0050933.1"
                     /translation="MKENIEITRFTNCLTILTEKMPDVRSATLGFFFKKGSRHEPAEL
                     NGISHFIEHTVFKGTEKRSALEIAVETDRLGGNFDAFTMQEMTGFAMKTVDKDLPRAF
                     DLLADMLTNPRFDQTELEREQQVIIEEMRMVEDSPEEFLGELFNQAIFPKHPLGLPIE
                     GTEKTVRTFSREVTTAFHGRVFQPENLVIAVAGNVEHAPMVELAEKFFGQSSAAGGQP
                     PEFEDSGVPQISAPILLKKKRGLEQAHFVLATPWLDARSERRYAAHLLENILGNGTSS
                     RLWQTIRERHGLAYSVGASGVSFLDCGFFSVYAGTAPNQIGKAIDLSVAELKRIKREG
                     VTAEEIELAKQQSVATILLGLEDSSNRTANLAQQEITFGKQIPLEETLRNIENVTAAE
                     IRALALEFFQTERMALAVLGNLNGLKIDRERLDVS"
     gene            complement(8421..8684)
                     /locus_tag="VGB68_16695"
     CDS             complement(8421..8684)
                     /locus_tag="VGB68_16695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050934.1"
                     /translation="MTYVLIKLNQAKEILASFENDRIEIGVPESFAEQWTKTSEVGFG
                     AEQKLSGGETLKILVEKDFACLAVREDEDETDAFPHPQAGQTC"
     gene            complement(8920..9162)
                     /locus_tag="VGB68_16700"
     CDS             complement(8920..9162)
                     /locus_tag="VGB68_16700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050935.1"
                     /translation="MSGYDFDKYFPLQGTCFHCGRDLRHHTLEKITERFAFGESVNKL
                     ADDYNVPPQAIELAIARNSTYPLGRTITPMVRARAA"
     gene            9311..>9788
                     /locus_tag="VGB68_16705"
     CDS             9311..>9788
                     /locus_tag="VGB68_16705"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0050936.1"
                     /translation="MNKITRRELLRLAGAWAAASLFINCAGAPEKQTIITNSNEPTRT
                     PQGRILPPDAAPPEKQVLYESANEPRHLDIARDIYGAGVAINWGGEPLLRRDQNQNLV
                     PAMAESWQAGENMEFWDFTIRAGSRWSDGVPITAHDWVFTFRHLASPQLDNPWTWFY"
BASE COUNT         2410 a         2456 c         2520 g         2402 t
ORIGIN      
        1 atgcggaacg gaagccggtc gaggcgtgca attttgattt gatggaaaaa ctcgagactt
       61 tctacagcgc cgcgccggac aagttccggg acgcggaagc ggattcgcag tttctgaaag
      121 aattaaaaac gtgcccgcgc gagcagacga taaattcgat tgccgcgctg caaaaggcgc
      181 gtcgggaaga cggcgagata caagccaaaa ccgcttatct gcttattaag ctcgaccgcg
      241 ataagaaagt taatcaaaaa gcgcttttct ccgtatttcc cgcttacaga gataaaaccg
      301 gcggcagatt tcgccgcgac cacatactcg acctgattgg cgacgtggtt ataaacgagg
      361 atacgggcga aaagaggttt ctcgccgacg cttttgattt ggctccgctg gttaacgccg
      421 cgacggcgga aatgctctcc ggcattctgg caggcgagtt tgaaaaaaat cccgaaatct
      481 ttcttctcag cttaaaaaac aaaccgaaag cggttaagcg cgaagtatat tcttttatct
      541 cttaccagct gccgaaagac gttttatcaa aacatttatc ctcgattccc gaaacgtccg
      601 acgcttaccg gacaggcaaa gaaatgtcgg agtttgccga aaacaggaag cgggtttccg
      661 gataattaca atcagccgta tcgggtcgtg ttttttgaca aaacggctga tacgattcga
      721 ggtttcaaaa ttaaatgttc aaaaacagtt tattctccgc tcctgtaagt ctttgcgcgc
      781 tgattctttt ttttgctttt tccgcttttg cgcagacgaa aattcccgag ccgaaagacg
      841 ttctcggctt tacgccgggc gacgaccgca agctcgcttc gtggaatcag acggtcgatt
      901 attttcagaa gctggatgcg gcgagcagcc gcgttcaatt tcaggaaatc ggcagaacga
      961 cgctcgacag accttttgtc tacgccgtaa tttccgcgcc ggaaaattta aaaaaccttg
     1021 agaaatacaa agcgataaac gccaaacttg ccgacccgcg ccggattatg cgttcgcgcg
     1081 cgtcgccggc gggcatcgag cgccagttgt taaaggacgg caaaacgatt gttttaatca
     1141 cctgcgggat tcattcgacg gaagtcggct cgacgctttc ctcgatgctg atcgcgcacc
     1201 ggctggcgtc ggcgggcgag ccggaaattc aaaagattct gcaaaacaca atcattctgc
     1261 tcgtgccgtc tttaaacccg gacggcgtcg atatcgtcaa aaattggtat gacaaaactt
     1321 tgggaacgcc gtttgaagga acttcgccgc cggagcttta tcaccggtac gtcgggcacg
     1381 acaacaaccg cgactggtat gctttcacgc aagtcgaaac gcagctgacg gtcgataaaa
     1441 tccacagcga atggcatccg caaatcgtcc acgacataca ccagcagggc gagcgcggct
     1501 cgcgtctttt tctgccgccg tatatgcagc cggtcgagcc gaacgtgccg caggaaatcg
     1561 tcgaaggcta cacggagctc ggcaatttta tggctgacga tttgcgcgcc aaaggttttc
     1621 agggaattac gaccaactcg acctacgacg cgtggacgcc cgcccgcgct tattcgcatt
     1681 atcacgccgg cgtgcggatt ttgtcggaga cggcttcggc gaaattagcg acgccggtaa
     1741 gcgttaaatt tgaagacctg cgaaacggtc tcggctacga cgtgcgaaag gaatcgacga
     1801 atttttcgcc cgtctggctc ggcggcgaat ggaaattgcg tgacattaca aactatatga
     1861 cggcggcgac tttttcttta ttgaatcacg ccgcggataa tcgcgcagcg tggctcggga
     1921 gattttacgc cgtcggcaag gaagccgtca gaccgcgccg cgccggagag ttgtttgctt
     1981 acgttttgcc gtatccgaaa agcgcggaga gcgaaagcgg ttccgaagaa tattttcgtc
     2041 gcgaagagct tctcggaatc ttccggcgcg ccggtgtcga aacaaaattc gccgtcaatc
     2101 ttcgaataaa cggggaaaaa tatcctgccg gaaccgttgt cataccgatt gcccagccat
     2161 acggaaattt cgcaaaggct cttttagaaa aacaggttta tccgaatttg cgggacgaaa
     2221 aaggaaatcc cgtcgcgccc tatgacgtga cggcgcacac cctctcgctt ttaatgggag
     2281 tcgaagcaaa gccgatttac aagtctttcg cttataaagt cacgaaaaat gggcttgaac
     2341 aaggtcgcgg ctgcggctgc ggcggcggca gctcagaacc cggttatctt atttaccagt
     2401 caaaggtcgc ttcgatggat gaaggctgga ctcgctgggt gaccgagaat gcggagcgat
     2461 ttcttaaccg atgctgcttc gccggcggtt ccgtcaccga ccgggaaata aagagtcaga
     2521 cgtttaaaag caattacata atctttcccg accagtcgtc gaatcaaatc ctgaacggct
     2581 acgcgaaagg ctcgatgccc gacgaataca cgggcggcgt cggcgcggaa ggcgtgaaaa
     2641 atctgcgacg ttttgtcgaa gacggcggaa cgctcgtttt cctgaatcgc gcctcggatt
     2701 tcgccatcaa agagctcggc gcgcccgtcc gcgacgtgac cgcgggcttg cagcgcaggg
     2761 atttttatat tcccggctcg attttaaaaa cggaaatcga caccgcccac aaaatcgccg
     2821 cagaaatgcc gaaagaatca atcgcgtggt ttgaaaactc gcccgccttc gagattgccg
     2881 gcgacgccgc aaacgtcgaa atcgtcgccc gctatcccga aaaagccgaa aacattctgc
     2941 tttccggctg ggcgctcggc gcggaaaaga ttgccggaaa agcggcgctc gtttcgataa
     3001 atatcggcaa aggcaaaatt atacttttcg gctttcgccc gcagtatcgc ggtcaatcgc
     3061 tggcgacctt tccgcttttc tttaatgcga tagcgcagtg aaaaacgaat aaccgaaagt
     3121 aaaaaacgcg cggcgaaaag tttttcactt tttactaaat ttatgtttct attcgacgac
     3181 atcgatttcg ttccggcgca gagcggcgag ctgagccgtt tgtggtattt tttgcccatc
     3241 ggctatctga cgacgatttt gattgaaacg ccgattttgc taatcggtct ttcgccgaaa
     3301 ttgagtttaa agcaaagatt cgcctgcggc gtttggctga cggcttgcac ctatccggtc
     3361 gtcatactgg tgctgcccgc gcttctgctc gacggctccg accgttcaag actcgtctac
     3421 ctggcggtcg ccgaaacttt cgcgcccgca gccgaatgtt ttttcttctg gctcgccttt
     3481 cgcggaaaaa atttgtttga gcagaaggac tggattcgct cgtttgcggc aatcgtcgtc
     3541 gccaatctgg cttcgttcgg catcggcgag attttaaacg ccgccgcgtg gttcggattg
     3601 ttttaacttt cgagtaagat tcaaatacga taaaagaaat aatgaatgac taacggcgaa
     3661 ttccgcattc atcattccgc gtttcaatta tggatttgcg cgtaacggaa atttttcttt
     3721 cgatccaggg cgaatcgacg cacgccggtc gcccgtgcgc cttcgtgcgt cagacgggct
     3781 gcccgatgcg ctgccgctgg tgcgattcgg aatacacttt tacgggcggc gagcgcctga
     3841 gcttcgatga aatcttcggc agattaaaag atttcagctg ccgcctggtc gaggtaacgg
     3901 gcggcgagcc gctcgcgcag aaaaacgttt ttccttttat cgagcggctc tgcgacgccg
     3961 gttacgaggt tttaatcgaa accggcggct ttctttcgac ggaaaaaatc gacgcgcgcg
     4021 caaagataat tttagacgtc aaatgtccgg cttcgggcga agccgaacgc aatcactggg
     4081 cgaacttgga acgcttgcgc gccgcgagcg acgaaataaa atttgtcatc gccgatttaa
     4141 gcgattggga atttgcgcgc cggattatcg aaaaatacga tttggaaaac cgcgcgcgca
     4201 gcgttttgat ttcgcccgtt ttcggcgtgg aaaatttacc ggaaatcgcc gaaaccgtct
     4261 cgcgaagcgg cttaaaggta agattaaact tacaactgca caaatacatc tggggcgcgg
     4321 acgtccacgg agtttagctt atgacgcatt acgaaaaact ttcgacgatg ttttttcgca
     4381 ttttgggcgc ttttcttttg cttgtcggaa taataatcgc cgttacggcg ctgattacga
     4441 taccggtcaa cggacttttt ccgccggttt tctttatcat tttttattcg attccgctaa
     4501 tggttttagg aattttattt ttttccgcga gccgaaaacc ggcaaaatgg gtttgctttg
     4561 atttcgataa aattaatgaa cgataaaaaa actgcaattt gtcttgtctc cggcggaatg
     4621 gattcgtgcg tcacctcggc gattgctaaa tccgaaaacg aggaaatcgc gcttctgcac
     4681 gtctcctacg ggcaaagaac cgaaaggcgc gagcgaaaag cgtttcagga aatcgccgat
     4741 ttttacggcg cgacgcggcg gctggcggtc tcgattgaat atcttgcccg aatcggcggt
     4801 tcgagcctga ccgacgaatc aatcgaaatc gccgaagcga atctcgaagc gtcggaaatt
     4861 ccgacttctt acgttccttt tcgcaacgcg aatatgctct cgattgcgac gagctgggcg
     4921 gaagtcgccg gcgcaaatcg aatctacatc ggcgcggtcg ccgaagattc gagcggttat
     4981 cccgattgcc gtccggaatt ttacgaggcg ttcgaaaaag cgattgacgc cggaacgaag
     5041 ccggaaacgc gcatcgaaat ccgcacgccg attattcatt tatcaaaagc ggaaattgtc
     5101 cggaaaggct tggagttaaa cgcgccttta cacctttcgt ggtcgtgtta tcgaaacgag
     5161 gatttggctt gcggaacgtg cgattcctgc gcgctgcgcc tgcgcggatt taaactggcg
     5221 ggcgtcaaag acccgattgc atacgaaaat tattgaagct ttttttcaat cttttccagc
     5281 cggaagtctt tgtgttcgac cagaagattc tgaaaatttt cgttcatcgt cacggctcgt
     5341 ccgccttttt caagattgcc ggtcgatttg tctttcgctt caagattatc tctgaatcgc
     5401 cctgaatatc gaaaaacgac cgtccagcct tttttgtttc cgcgagcgcc gggcgaaatg
     5461 ccgtaagctt tcggctccag agaattgcgt ctttgtttga gcaaatcgtc cgggtcttca
     5521 taaaattcga ccgtttcgcg cgacaaatta ttttcgtccg ccgcggcgtc cgtatagccg
     5581 ttttgccgga cgaattcttc ggcgaggcgg acggcttttt cttccggcgt ttccgctttt
     5641 tgcgaaaaat tttcagattg tctcgtgacg gtttcaattt cggcggtatt tcgttcgact
     5701 tttgcccgtt cgcaattcgc ggaaaataaa aaaccgaaga ttaaaaagct caggaatccg
     5761 gcgtatttca taaattactc ccgatgatga acgggtagat ttatcataaa ttgcgcataa
     5821 accggcaaaa cattctatct tttttctgat tttaaacgtt tgaatttccc gaaattcccg
     5881 gcgggtttcg gcgaaagcga aagagcggat taattataaa aattttcagg caggctgctc
     5941 ggctcttaaa acatcttcct tttgtttttc gaggcgggta atctgcgctt tcagcccggc
     6001 gattttcgct tcgatttcgg caaccgacgg aatatcgaca aattcggtgc ggctcaggcg
     6061 gtgaaaaatc ggaatcatca ccgtcagcgc ggcgtcgatt acggctaaaa cgccgattag
     6121 acgtaaaact attccgcttt cgctgggcgg ttcgaaaacg ataataaatg aaattatcga
     6181 agccagcgcg aaaatgacga cgaaggcggt aatcagcgac cagcgaaaac gcctggaaag
     6241 ctgcgcgata gataaaagcg acaggtgcga gagcgcaaac gcgaaaatcg aagaaacggc
     6301 gagggtctta aaaagaactt catcagacca gtttgttttc atccaaatca gccagattgt
     6361 aatcagcgcc gacaaaacgg agagaattat tccgacaacc ggaatggcga tgagcggaaa
     6421 tttctgcgag cgcggatttt cgagaaaagc gccgcaggct aaacccagaa tgctggttcc
     6481 gacaatcgtc agagtcgtgc ccagaacccg atattccacg tcgcccgaga gaatcgccca
     6541 gatgcccatc aacgcgctca gagcaatcga gcaaatcatc aggtaaagaa agattttttt
     6601 aacgttaagg ctttccacgc cgccatttta tgccggatgc ggacggctgt caataaattt
     6661 tattgaattt aattttttgc tctggtagga attaaaagca gaatacagcc gataaaaaga
     6721 aaaaatatcc cgacaaagga aagcaggacg ccttgataag ccgaatcgat tgccgtcgca
     6781 aacgcaccga tgcccgaagc ctcgctgctc ttcatcgcgt taaagctgcc gaaaaaattc
     6841 cacagcgcgt aagagtcggc gacgaagact ataaaagttc cgataattat aagcagtttt
     6901 gaaatggttt tcatatttag tccgaaaaga tttcgaagag tttaacttat cttttctcaa
     6961 caatccagag aagtttcacg aaacgtcgag cctttcgcgg tcgattttta aaccgttcag
     7021 atttcccaaa accgccagcg ccatcctctc ggtttgaaaa aattccagcg ccagcgcgcg
     7081 gatttccgcg gcggtcacgt tttcgatatt tcgcagcgtt tcttcgagcg gaatttgttt
     7141 gccgaaagtg atttcctgct gcgcgagatt cgccgtgcgg tttgacgaat cttcgagtcc
     7201 gagcagaatc gtcgccacgc tctgctgttt ggcaagctcg atttcttccg ccgtaacgcc
     7261 ttcgcgcttg attcgcttca gttcggcgac ggataaatca atcgctttgc cgatttgatt
     7321 cggcgcggtt ccggcgtaaa cgctgaaaaa tccgcaatcg agaaacgaaa cgccgctcgc
     7381 gccgacgctg taggcaagcc cgtggcgctc gcgtatcgtc tgccaaaggc gcgacgaagt
     7441 gccgttgccg agaatgtttt cgagcaggtg cgccgcgtaa cgccgctcgc ttcgcgcatc
     7501 gagccacggc gtcgccaaaa cgaaatgcgc ctgctccagc ccgcgtttct ttttcagcag
     7561 aatcggcgcg gaaatttgcg gcacgccgga atcttcaaat tccggcggct gaccgccggc
     7621 ggctgacgac tgaccaaaaa acttttccgc cagctcgacc atcggcgcgt gctcgacgtt
     7681 tccggcgacg gcgatgacca gattttccgg ctgaaaaacc cgcccgtgaa aagcggtcgt
     7741 cacctcgcgg ctaaaagtgc gaacggtttt ttccgttcct tcaatcggaa gcccgagcgg
     7801 gtgtttcgga aaaatcgcct gattaaaaag ctcgcccagg aattcttcgg gcgaatcttc
     7861 gaccattcgc atttcttcga tgatgacctg ctgctcgcgt tccagctccg tttggtcaaa
     7921 gcgcggattg gtcagcatat cggcgagcaa atcaaaggcg cgcggcaaat ccttgtcgac
     7981 cgttttcatg gcgaagccgg tcatttcctg catcgtaaaa gcatcgaaat ttccgcccaa
     8041 acggtcggtt tcgacggcga tttccagcgc ggaacgtttt tccgtgccct tgaaaacggt
     8101 gtgttcgata aaatgcgaga ttccgttcag ctcggcgggc tcgtgacgcg agcctttttt
     8161 gaagaaaaat ccgagcgtcg ccgagcgcac gtcgggcatt ttttcggtga gaatggttaa
     8221 acagttggta aagcgtgtga tttcgatatt ttctttcatt gcggcaaaag aaaatcgtat
     8281 cacggaaacg cggaatgatg aatgcggaac gatgaagtga gttttgaatt ttgagttttg
     8341 aattaatgat tcacggttaa cttaaaactc aaaactttct tttgttcatc gttcatcgtt
     8401 cctgattcat catttctctt ttaacaggtt tgaccggctt gcgggtgcgg gaaagcgtcg
     8461 gtttcgtcct cgtcttccct gacggcgagg caggcaaaat ctttttcgac gaggattttg
     8521 agcgtttcgc cgccggataa tttttgctct gcgccgaagc cgacttccga ggtttttgtc
     8581 cattgttcgg caaacgattc gggaacgccg atctcgattc ggtcgttttc aaagcttgcc
     8641 agaatttctt tcgcttgatt tagcttaatt aagacatacg tcaatcgccg atttccgaaa
     8701 gaaatcgaat cttcgacgcg tgagtttcgg gcaaagtcgg aaacttccgg ttgcgataat
     8761 ctgaggcgga tcgaattgcc tttgattctc aatttcatat ttcaaaagca attgtaaacg
     8821 ggcgcggcgc aaaataaaaa cccgcgtcgg ctcgggaatt ttcaaccggg aaaattcact
     8881 aagccggtgc gggtaaaatc aagtaagtcc gcctgcggtt caggcggctc tggcgcgcac
     8941 catcggcgtg atggttctgc ctagcggata agtcgaattg cgcgcaatcg ccagctcaat
     9001 cgcctgcggc ggcacattgt aatcgtcggc aagcttgttg acggattcgc cgaaagcaaa
     9061 gcgctcggta attttttcca gcgtgtgatg ccgcaaatcg cgcccgcaat gaaaacacgt
     9121 tccctgtaaa ggaaaatatt tatcgaaatc gtaaccgctc ataatcagac ccccgggaat
     9181 tttattggct ttgggaaaat taataatagc agaagccgcg ccgataaaca atattttttt
     9241 aaggcgactt gaaaatcctt taaaaattat atattaaata tgaaccgatt ttataaatcg
     9301 atttcgttaa atgaataaaa tcacccgtcg cgaattatta cgtcttgccg gggcttgggc
     9361 tgccgcaagc cttttcatca actgcgcggg cgcgccggaa aaacaaacga ttatcacaaa
     9421 ctcaaacgag ccgacgcgga cgccgcaggg acgaattctg cccccggacg ccgcgcctcc
     9481 ggaaaaacag gttttgtatg aatcggcgaa tgagccgcgt cacctggaca tcgcgcgcga
     9541 tatttacggt gcgggcgtgg cgataaactg gggcggcgag ccgcttctgc gccgcgacca
     9601 gaatcagaat ctcgttccgg caatggcgga aagctggcag gcgggcgaaa atatggagtt
     9661 ttgggatttt acgattcgcg ccgggtcgcg ctggagcgac ggcgtgccga ttacagcgca
     9721 cgattgggtt ttcacttttc gacatctcgc ttcgccgcag ctggacaatc cgtggacttg
     9781 gttttatt
//
LOCUS       DASWNE010000857         4936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_206241, whole genome shotgun sequence.
ACCESSION   DASWNE010000857 DASWNE010000000
VERSION     DASWNE010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206241"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="VGB68_16710"
     CDS             <1..779
                     /locus_tag="VGB68_16710"
                     /inference="COORDINATES: protein motif:HMM:NF024573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0050937.1"
                     /translation="WLIRSHYESLLKSEFQEKRKQKLDKVIATLIKILPDINEIRFSE
                     KPKNNNSQPIVEFHTSYGRVEAKYLSLGYRTSMAWMIDFAARMFDLYPESKNPLSEPA
                     VVLIDEIDLHLHPKWQRELIDYLTNLFPNTQFIVTTHSPLIVQAAVARNANIVVCRRS
                     GDHVVIEQITENLKNMRVDQILTADYFNLPTARPKEIEKLLKEKEDILSKSKLTKKDE
                     KRLKELEEEIGFLPASESHEDNEAMEVIRNAADWLKTQAK"
     gene            789..1487
                     /locus_tag="VGB68_16715"
     CDS             789..1487
                     /locus_tag="VGB68_16715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050938.1"
                     /translation="MISIKKPKTAPKVLQEKGKTESESLCQKYEDGEREFEFNSDIYG
                     HKEVKEKLIKAQKEKCFLCESRVRHISYGDVEHFRPKAAYCQDDADEMHKPGYYWLAY
                     NWKNLFLACQICNQRFKKNLFPLEDNSKRVTSHSEDINQEKPLFINPVEDNPEDFISF
                     RGVMPFAINGNLKGQLTIENAGLKRPELVENRRELYGKMKALYNLAHGYPETPQREQA
                     MRVLKKRAAKMPIT"
     gene            complement(1532..2035)
                     /locus_tag="VGB68_16720"
     CDS             complement(1532..2035)
                     /locus_tag="VGB68_16720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050939.1"
                     /translation="MPGEIRGYKVHRAKVSVQTETGENNPKEDFSVVVNFDEPKLVKV
                     SPLGATFEVFGEMTVFEQSGKIDFLTFKDFRVNGLKVEIEEYREFFEIEKNKPARLKK
                     PFKIFVSAAQSIKGGLKEIFRSEKHWQVTGRVFVFGKFKKLGFKFKRVVPVDVNLKIV
                     NPLKLST"
     gene            complement(2200..3426)
                     /locus_tag="VGB68_16725"
     CDS             complement(2200..3426)
                     /locus_tag="VGB68_16725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0050940.1"
                     /translation="MEELREAFLIDSISKLDNLQDVLQSEAFSPELQREIFRALHTIK
                     GTAQTFGLNAAARLAHGIENLLEAARDNQIQSDEKTISLLREGLKYLSDALKSAGETD
                     FPQEFADKIGQIVKIDFPAPKDFLVQKIPPEFLARLSPHEQNQIATALENGKNLFLIE
                     VGFEFAGFDEDFKKLKNTLSETGDAAAAFPSPKFADKIGFQIFFITEIKPDAVAGLVA
                     PFGADIIFHLVKPETAFPTNPQGVLRQAVEGGRETARRLGKNVEFDLSLDKPEASVKL
                     LKTLSEVLLHLVRNAIDHGIDRDGKIKIELASKPGGVFLKVSDDGRGIDLEKVRRKAI
                     EKNLIAEDADLSEAEILNLIFTHGFSTGAAVSEISGRGVGLDVVNDLTEKSGGEITVK
                     SERGRGTTFEILFKEK"
     gene            complement(3460..3927)
                     /gene="nusB"
                     /locus_tag="VGB68_16730"
     CDS             complement(3460..3927)
                     /gene="nusB"
                     /locus_tag="VGB68_16730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEY0050941.1"
                     /translation="MGTRRKARECALQMLFAADVTKTSENVLTRYYWHELGEPEIDEQ
                     TREFANNLAVGTLRQMDAIDERIRSRAEHWRIPRMAIVDRNILRLAVYEFLFEQTPHT
                     VVINEALEIARRFSTFEATQFINGILDAIKHDLEKNPVNKPPEGESQHASSSK"
     gene            complement(3966..4463)
                     /gene="ribE"
                     /locus_tag="VGB68_16735"
     CDS             complement(3966..4463)
                     /gene="ribE"
                     /locus_tag="VGB68_16735"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF000812.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEY0050942.1"
                     /translation="MQPKVHQGLLTAEGFRFAIVSARWNDFLTARLTEGALDALERLG
                     AKETAVEIFKVPGSFEIPLTAQMVAESGRFDAVICLGTVIRGQTPHFDFVAGEAAKGI
                     GQVGMRTGVPVLFGIVTADTLEQAIDRAGVKAGNKGFEAAMSAVELVNLYKEMQGGKN
                     ENRRK"
     gene            complement(4642..>4936)
                     /locus_tag="VGB68_16740"
     CDS             complement(4642..>4936)
                     /locus_tag="VGB68_16740"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY0050943.1"
                     /translation="QIGEFSAAEALKSGLINKITNSEEVETEAFALAEKISRLAPLAI
                     RACLKAVAEGSHSSLEEGLKIDIELFSQIFSTADMREGTRAFLEKRKPVFTGE"
BASE COUNT         1349 a         1128 c         1099 g         1360 t
ORIGIN      
        1 aatggctcat tcgctcacat tatgaatcat tacttaagtc agaatttcag gaaaaacgaa
       61 agcaaaagtt agataaagtt attgcaacac ttataaagat tttgccggac attaatgaaa
      121 ttaggtttag tgaaaaacct aaaaataata attctcaacc aattgtcgaa tttcatactt
      181 cttatggtcg agttgaagct aaatacttaa gccttggtta tcgaacttcg atggcttgga
      241 tgattgattt tgccgcgcga atgtttgacc tttatcctga aagcaaaaat cctttatctg
      301 agcctgctgt tgttttgatt gacgagattg atttgcattt gcatccgaaa tggcaacgcg
      361 aactgattga ttacttgact aatcttttcc caaacacaca gttcatcgtt acaacacata
      421 gcccattaat tgtgcaggct gctgtcgcac gtaatgccaa tatcgttgtg tgtcggcgta
      481 gcggtgacca tgttgtgatt gaacaaataa ctgaaaattt aaaaaatatg cgggtcgacc
      541 agattttaac ggctgattat tttaatctac cgactgccag acctaaagaa atcgaaaaac
      601 ttctaaaaga aaaagaggat attttgtcta aatctaaact aacgaaaaaa gacgaaaaac
      661 gtctgaaaga actcgaagaa gaaatcggat ttttacctgc cagtgaaagt cacgaagata
      721 atgaagcaat ggaagttatc agaaacgcgg cggattggtt gaaaactcaa gccaaataat
      781 ctctcactat gatttcgata aagaagccga aaacagcacc gaaagtttta caggaaaaag
      841 gaaaaactga gtctgaatct ttgtgtcaaa aatacgaaga cggcgaaagg gaatttgagt
      901 ttaattctga tatttatgga cacaaagaag taaaagagaa gttaataaaa gcccaaaagg
      961 aaaaatgttt tctttgtgaa tcaagagttc gccatatttc ttacggcgat gtagaacatt
     1021 ttcgacctaa agcagcttat tgtcaggatg atgcagatga aatgcacaaa cctggttatt
     1081 attggctggc ttacaattgg aaaaatttgt tcctagcctg tcaaatctgc aatcagcgct
     1141 ttaagaaaaa tctttttcct ttagaagata atagtaaaag agtgacatct catagtgaag
     1201 atataaatca agagaaacct cttttcatta atccggtaga agataacccg gaagatttta
     1261 tttctttccg tggagttatg ccttttgcta taaacggaaa tttaaaaggt cagcttacca
     1321 tcgaaaatgc aggtttaaaa agacctgaat tggtcgaaaa tcggagagag ctttacggga
     1381 agatgaaggc tctttacaat ttggctcacg gctatcctga aacacctcaa agagaacagg
     1441 ctatgagagt tctaaaaaaa agagctgcga aaatgccaat tacctaaaca tcaatatata
     1501 gcaatgttca atgcagcaat aagagacaat tttaagtact aagttttaat ggatttacga
     1561 ttttcagatt cacgtccacc ggcacgacgc gtttgaattt aaagcccaat tttttgaatt
     1621 tgccgaaaac gaaaactctg cccgtcacct gccaatgctt ttcggagcga aaaatttctt
     1681 ttaagccgcc tttgattgat tgcgcggcgc tgacaaagat tttaaacggt ttcttcagac
     1741 gcgcgggttt gtttttctcg atttcaaaaa actctctata ttcttcgatt tcaaccttta
     1801 aaccgttgac gcgaaagtct ttaaaagtta aaaaatcgat ttttccgctt tgttcgaaaa
     1861 cggtcatctc gccgaaaact tcaaaggtcg cgccgagcgg cgagactttg acgagtttcg
     1921 gttcgtcgaa attaaccacg acgctgaaat cctctttcgg attgttttcg ccggtttcgg
     1981 tctgcacgga aactttcgcc cggtggactt tgtaaccgcg aatctcgccg ggcaaatcct
     2041 gcgctaagaa tgaaatagca aaaagaaaaa taaagccgca gaggcacaca aaagcagcga
     2101 gtttacgcgc aaaaggcaaa agggaaaagg taaaaagaga gaatttgttt cttaattttg
     2161 ccttttgcct tttccctttt gccttgtttt ctttgtattt catttttcct taaataaaat
     2221 ctcgaatgtc gttccccgac cgcgttcgct tttgactgta atctcgccgc cggatttttc
     2281 cgtcaaatca ttgacgacat cgagcccgac gccgcgcccc gaaatttccg atacggcagc
     2341 gccggtcgaa aaaccgtgcg tgaaaattaa attcaaaatt tcggcttccg ataaatcggc
     2401 atcttccgca atcaaatttt tctcgattgc tttgcgcctg actttttcca aatcgattcc
     2461 gcgcccgtcg tccgaaactt tcaagaaaac gccgcccggt tttgacgcca gctcgatttt
     2521 tattttgccg tcgcggtcga tgccgtggtc aatcgcgttg cgaaccagat gcaaaagaac
     2581 ttccgacaaa gttttcagaa gctttaccga agcttccggt ttatcaagcg agaggtcgaa
     2641 ctcgacattt tttccgagcc tccgcgccgt ctccctgccg ccttcgaccg cttgccgcaa
     2701 aacgccttgc ggattggtcg gaaaagccgt ttccggtttt acgagatgaa aaattatatc
     2761 tgcgccgaaa ggcgcgacca gcccggcgac agcgtccggc ttgatttcgg tgataaagaa
     2821 aatttgaaaa ccgattttat ccgcaaattt cggactcgga aaagcggcgg cggcgtcgcc
     2881 cgtttcgctc agagtgtttt tcagcttttt aaaatcctcg tcaaagcccg caaactcgaa
     2941 gccgacctcg attaaaaaaa gattttttcc gttttcgagc gccgtcgcaa tctgattttg
     3001 ttcgtgcggg gaaagccggg ctaaaaactc gggcggtatt ttctgaacga gaaaatcttt
     3061 cggcgcggga aagtcgattt tgacgatttg cccgatttta tcggcaaatt cctgcggaaa
     3121 atcggtttct ccggcagatt taagagcgtc gctcaaatac ttcaagccct cgcgcaaaag
     3181 ggaaatcgtc ttttcgtcgc tttgaatttg attgtcgcgg gcggcttcga gaagattttc
     3241 gattccgtgc gccagacgcg cggcggcgtt taaaccgaaa gtttgcgcgg ttcctttaat
     3301 cgtatgcagc gcgcgaaaaa tttcgcgctg caattcgggc gagaatgctt cgctttgtaa
     3361 gacgtcttgc agattatcta attttgaaat tgaatcaatg agaaacgctt cacgaagttc
     3421 ttccatcggt ttttcttttg ccggaagaat tttctggaat tacttgctcg acgaagcgtg
     3481 ctggctttcg ccttccggcg gcttgtttac gggatttttt tctaaatcgt gtttgatggc
     3541 atcgagaatg ccgttgataa actgcgtcgc ttcaaaggtc gaaaaccggc gcgcgatttc
     3601 cagcgcctcg ttgatgacga ccgtgtgcgg cgtctgctcg aacagaaatt cgtagacggc
     3661 gagccgcaaa atattccggt cgacaatcgc catccgcgga atgcgccagt gttccgcccg
     3721 cgagcgaatc cgctcgtcga tcgcgtccat ctgccggaga gttccgaccg ccagattatt
     3781 ggcaaactcg cgcgtttgct cgtcgatctc cggttcgccg agctcgtgcc agtaatagcg
     3841 cgtcaaaacg ttttccgacg ttttggtcac atctgccgca aataacattt gtaaagcgca
     3901 ttcacgcgcc ttacgtctgg ttcccatagc ccttgattat tttcagtgaa tcggcggaaa
     3961 atttattatt ttcgtcggtt ttcatttttt ccgccctgca tttctttata taaattgacc
     4021 agctcgactg ccgacatcgc ggcttcaaag cctttgttgc ctgctttaac gccggcgcgg
     4081 tcaatcgcct gttcgagcgt gtcagccgtg acgatgccga ataaaaccgg cacgcccgtg
     4141 cgcattccga cctgcccgat gcctttcgcc gcttcgcccg ccacaaaatc gaaatgcggc
     4201 gtttgcccgc gaatcacggt tcccagacaa atcacggcgt cgaatctgcc gctttcggca
     4261 accatctgcg ccgtcaaagg aatttcaaac gaacccggga ctttgaaaat ttcaaccgcc
     4321 gtttcttttg cgccgagtct ctccagcgcg tcgagcgcgc cttcggtcag ccgcgccgtc
     4381 agaaaatcgt tccagcgcgc cgagacaatc gcaaaacgaa atccttctgc cgttagtagt
     4441 ccctgatgaa ctttgggttg caaaattttc tccttcaaac gagcaaagct tcagatgttt
     4501 agtataggag agatttgcgg agcgtgacaa gagccgaaaa gggaaaagtg agcggtaagc
     4561 agtaagcggt gagcagccgg gttttgaaaa tcgatgttga tttcttaaac tctttactgc
     4621 ttactgttta ccgcttactg ctcactctcc cgtgaaaacc ggctttcgtt tttccagaaa
     4681 agcgcgtgtg ccttcgcgca tatcggcggt cgaaaaaatt tgcgagaaaa gctcgatatc
     4741 gattttcaag ccttcttcga gcgacgagtg cgagccttcg gcgacggctt tcagacaggc
     4801 gcgaatcgcc agaggcgcaa ggcgggaaat tttttcggcg agcgcaaaag cttccgtttc
     4861 gacttcttcg gaattcgtta ttttatttat taagccggat tttaaagctt cggcggcaga
     4921 aaattctccg atttgc
//
LOCUS       DASWNE010000858         4343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_168215, whole genome shotgun sequence.
ACCESSION   DASWNE010000858 DASWNE010000000
VERSION     DASWNE010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168215"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VGB68_16745"
     CDS             complement(<1..659)
                     /locus_tag="VGB68_16745"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEY0050944.1"
                     /translation="MRVIIHLSDIHFGRVDYAVVERLREKIEEISPHLVVVSGDLTQR
                     ARSAQFIEAREFLESLPQPQIVVPGNHDVPMYNILARFFSPLEKYKRYITKDLQPFYA
                     DDEIAVVGVNTARSLTIKDGRINEKQVAEIREKMCDLNDKMLKIIVTHHPFDLPAGYA
                     ESDIVDNAAEAMPQIAECGADVFLSGHLHVSHISHTAERYKLDSGRSALVIQAGTATS
                     TR"
     gene            complement(750..1679)
                     /locus_tag="VGB68_16750"
     CDS             complement(750..1679)
                     /locus_tag="VGB68_16750"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEY0050945.1"
                     /translation="MPPSVEVIINAGSGSVEHEETRRRLAELFEENGVEANVSMAENG
                     DDITELAQRAAAGNAEIIVAGGGDGTISAVAVEAIKSKKTLGILPLGTLNNFSKDLRI
                     PQDLEEAVRVIAEKNTKQIDVGEVNGRIFVNNSSIGLYPSLVRRRERQQRLGYGKWRA
                     AFWAAFKILRRSPFLNVKLNIGGEEILRRTPFVFVGNNEYEMDFFNIGRRAKLDDGKL
                     SVYLLHRSGRSGLFLLALRMAFGRLRQAEDFEEINTEEITIETRRKRMPVAFDGEVAV
                     LQMPLNYIIHAQALRVIAPKENAEESPAGGKDG"
     gene            complement(1862..2698)
                     /locus_tag="VGB68_16755"
     CDS             complement(1862..2698)
                     /locus_tag="VGB68_16755"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: protein motif:HMM:NF008808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEY0050946.1"
                     /translation="MDLKHKIENLFEADKTEFDATDRAVFEDFKAALRRGEIRSAEPD
                     ADGNWKVNAWVKRGILLGFRMGKMVTMSFSGETFQFFDKETFPLRPMNLDDRIRIVPG
                     GSTIRDGSYVAPNVVLMPPCYVNVGAYVDEGTMIDSHALVGSCAQIGKRVHLSAAAQI
                     GGVLEPVSANPVVIEDDVLIGGNTGVYEGTIVRARAVLASGVILTRSTPVFDLVKSAI
                     YKSDGEKPLEIPAGAVVVQGARAVTSGFGKENGLSIYAPIIVKYRDERTDAATKLEDY
                     LR"
     gene            complement(2816..4018)
                     /locus_tag="VGB68_16760"
     CDS             complement(2816..4018)
                     /locus_tag="VGB68_16760"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEY0050947.1"
                     /translation="MKKILSVFLIFSFLVLSVGAQTKPRARGLGVPFEGATGQYNAIT
                     DVKGVEVGYVTLISGEGKKAVRTGVTAILPQGKDFKGRVFAAWHALNGNGEMTGTTWI
                     EESGGLGSPVMITNTHSVGVVRDAVIEWGTKRFAEGIGYSGDFSLPVVAETYDGFLND
                     INGFHVKKEHVFQALDAAKTGAVAEGNVGGGTGMMAHGFKGGTGTSSRVLDARFGGYT
                     VGVLVQANHGTRQFFSVAGVPIGKEITDLRPTLGQKNATLIGEKKSALNDEGQGSIIV
                     VAATDAPLLPHQLKRLVRRVSLGIGNVGGRGENSSGDIFIAFSTANTAEFGKNEGLAN
                     LTMLPNDRINPLFWATTQATEEAILNALVAAETMKGLNGNTAYALPQERLKEILKKYN
                     RLQETAKK"
     gene            complement(4029..>4343)
                     /locus_tag="VGB68_16765"
     CDS             complement(4029..>4343)
                     /locus_tag="VGB68_16765"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0050948.1"
                     /translation="VADAERFYKMLENASRTIAAFEGERIVGFARALCDDVSNGYIGT
                     IAVAAEHRRKGVGRELVERLVGDDKNITWVLRAGRGSEKFWKKMGFNFSETAMEKTRA
                     AK"
BASE COUNT          936 a         1267 c         1080 g         1060 t
ORIGIN      
        1 cgggtcgagg tcgccgtgcc cgcctgaatg acgagcgcgc tgcgccccga atccaattta
       61 taacgctcag ccgtgtgtga aatatggctg acgtgcaggt gtcccgacag aaaaacgtcc
      121 gcgccgcact cggcgatttg cggcatcgct tcggcggcgt tatcgacgat gtcgctttcg
      181 gcatagcccg cgggcaaatc gaaaggatga tgcgtgacga taattttcag cattttatca
      241 ttcaaatcgc acattttttc gcggatttcg gcgacctgtt tttcgttgat gcgcccgtct
      301 tttatcgtca gcgagcgcgc cgtgttgacg ccgacgacgg cgatttcgtc gtccgcgtaa
      361 aacggctgta aatcttttgt aatgtaacgt ttatattttt cgagcgggct gaaaaaccgc
      421 gccagaatat tatacatcgg aacatcgtga ttgccgggca cgacgatttg cggctgcggc
      481 agagattcta aaaactcgcg cgcttcgata aactgcgccg agcgggcgcg ctgcgtcaaa
      541 tcgccggaga cgaccaccag gtgcgggctg atttcttcga ttttttcccg cagccgctcg
      601 accaccgcgt aatcgacgcg cccgaaatgt atgtcggaaa gatgaattat tactcgcata
      661 tttaaaaaaa gaatttcgtt tgccgccaaa cgccggacac ggatttgaaa tcctaaattt
      721 gaaacggcaa aatcgcccgc ttcgccggct tacccgtctt taccgccggc gggcgattct
      781 tcggcatttt ctttcggcgc aatgacgcgc aaagcctgcg cgtgaattat ataattgagc
      841 ggcatctgca aaaccgcaac ttcgccgtca aatgccaccg gcattcgttt ccttcgcgtt
      901 tcgatggtta tttcttcggt gtttatttcc tcgaaatcct cagcctgccg gagcctgccg
      961 aaagccatcc gcaaggcgag cagaaacaaa cccgaacgcc cgctccggtg cagaagataa
     1021 acgctcaatt tgccgtcgtc cagtttcgcg cgcctgccga tattgaaaaa atccatttcg
     1081 tattcgttat tgccgacaaa cacaaacggc gtccggcgca gaatttcttc gcccccgata
     1141 tttaatttca cgtttaaaaa cggcgagcgg cgcaaaattt taaaagccgc ccagaaagcg
     1201 gcgcgccatt tgccgtatcc gagccgctgc tgtcgctcgc gccgccgcac gaggctcgga
     1261 tacaaaccga tgctcgaatt attgacgaaa attcgcccgt taacctcgcc gacgtcgatt
     1321 tgttttgtat ttttttcggc gataacgcgg acggcttctt ccaaatcctg cggaatccgt
     1381 aaatctttcg aaaaattatt gagcgttccc agcggcagaa tgccgagcgt tttcttggat
     1441 ttaatcgcct cgacggcaac cgcgctgatc gttccgtcgc cgccgcccgc gacaattatt
     1501 tcggcatttc cggctgcggc tctctgagcc aactcggtga tgtcatcgcc gttttccgcc
     1561 attgaaacgt ttgcttcgac gccgttttcc tcgaacaatt ccgccaggcg ccgtctcgtt
     1621 tcttcgtgtt cgacgcttcc tgaacccgca ttaataataa cttcgacaga tggcggcata
     1681 tctagagatt tcaacacaaa gagacgagga atacaaagaa gaatcagccg cagataaagg
     1741 cgggcagctc ggataagaaa ataatattat cctattcgca aggctgcgaa gcttattcca
     1801 tcgagccgcg aagcctgctt ttaatttccc tttgtgctct ttgcgtcttc gcgcccaaaa
     1861 actaccgcaa ataatcttcc agcttcgtcg ccgcgtcggt gcgctcgtcg cggtatttga
     1921 cgataatcgg cgcgtaaatc gacaagccgt tttccttgcc gaagccgctc gtaaccgccc
     1981 gcgcgccctg cacgacgacc gcgcccgccg gaatttccag aggtttttcg ccgtcggatt
     2041 tgtaaatcgc gctttttacc aaatcgaaaa ccggcgtcga gcgcgtcagg ataacgcccg
     2101 aagccagaac ggcgcgcgcg cgaacaatcg tgccttcgta aacgcccgtg ttgccgccga
     2161 ttagaacgtc gtcttcaatg acgaccggat tggcgctgac cggctctaaa actccgccga
     2221 tttgcgccgc cgccgaaaga tgcacgcgct tgccgatttg cgcgcacgag ccgaccagcg
     2281 cgtgcgagtc aatcatcgtg ccttcgtcga cgtaagcccc gacattgacg taacacggcg
     2341 gcatcaggac gacgttcggc gcgacatacg agccgtcgcg aatcgtcgag ccgccgggca
     2401 cgatgcggat acggtcgtcc agattcatcg gacgaagcgg aaaagtttcc ttgtcgaaaa
     2461 actggaaagt ctcgccgcta aacgacatcg tcaccatttt gcccattcgg aagccgagca
     2521 gaattcctcg tttgacccag gcgttcacct tccagtttcc gtccgcgtcc ggttcggcgg
     2581 aacgaatctc gccgcgcctg agcgccgctt taaaatcttc gaaaacggcg cggtcagtcg
     2641 catcgaattc ggttttatcg gcttcaaata aattttcaat tttatgtttc aagtccatct
     2701 ttatataact tccattttat ccgcatctgc ggcgaatcgc gatttgattg ctttccgcgc
     2761 tgcgggcgtc ataaaaaacg ccgaaccggc aaacatcggg ttgattaatc cgagcttatt
     2821 tcttcgccgt ttcctgcagg cgattgtatt ttttcaaaat ctctttcaat ctttcctgag
     2881 gcagagcata agccgtgttg ccgtttaagc ctttcatcgt ttccgccgcg accaacgcgt
     2941 tgagaatcgc ttcttcggtc gcctgcgtcg tcgcccaaaa caaaggattg attctgtcat
     3001 tgggcagcat cgtcaaattt gccaaacctt cgtttttgcc gaattcggca gtgttggcgg
     3061 tcgaaaaggc gataaaaata tcgcccgaag aattttcgcc tctgccgccg acattgccga
     3121 tgccgagcga gacgcgccgg acgagccgtt tcaattgatg cggcagaagc ggcgcgtccg
     3181 tcgccgcgac gacgataatc gagccttgtc cttcgtcgtt cagcgcggat tttttctcgc
     3241 cgattaacgt cgcatttttc tgtcccaaag tcggacgcaa atcggtgatt tctttcccaa
     3301 tcggaacgcc cgcgaccgag aaaaactggc gcgtgccgtg gtttgcctgc acgagaacgc
     3361 cgaccgtgta gccgccgaat ctcgcgtcca gaacgcgcga cgaagttccc gttccgcctt
     3421 tgaagccgtg cgccatcatt ccggttccgc cgccgacatt tccttcggcg accgcgcccg
     3481 tttttgccgc atccagcgct tgaaaaacgt gttctttttt aacgtgaaag ccgttgatgt
     3541 cgttcaaaaa accgtcgtaa gtttccgcca cgaccggcaa gctgaaatcg cccgaataac
     3601 cgatgccctc ggcaaaacgt ttcgtgcccc attcgataac cgcatctctg acgacgccga
     3661 cgctgtgcgt attggtaatc atcacgggcg agccgagccc gccggattct tcaatccacg
     3721 tcgtgccggt catttcgccg ttgccgttta aagcgtgcca ggcggcgaag acgcgccctt
     3781 tgaaatcttt gccctgcggc aaaatcgccg tcacgcccgt gcggacagcc tttttgccct
     3841 cgccggagat taaggtcaca tagccgactt cgacgccttt gacatcggtt atcgcgttgt
     3901 attgtccggt cgcgccttca aacggaacgc ctaagccgcg cgctcgcggc tttgtctgcg
     3961 ccccgaccga taaaaccaga aaagaaaata tcagaaaaac ggaaagtatt tttttcatca
     4021 ttttattctt attttgcggc gcgcgttttt tccatcgccg tttcggaaaa gttaaagccc
     4081 atttttttcc agaatttctc gctgccgcga ccggcgcgca aaacccaggt gatgttttta
     4141 tcgtcgccga cgagtctttc gaccagttcg cgaccgacgc ctttgcggcg atgttcggcg
     4201 gcgaccgcga ttgttccgat atagccgttg gaaacgtcgt cgcacagggc acgggcgaaa
     4261 ccgacgattc tttcgccttc aaacgcggcg attgtgcggc tcgcgttttc caacatcttg
     4321 taaaatcttt ccgcatcggc gac
//
LOCUS       DASWNE010000859         4248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_340139, whole genome shotgun sequence.
ACCESSION   DASWNE010000859 DASWNE010000000
VERSION     DASWNE010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340139"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VGB68_16770"
     CDS             complement(<1..375)
                     /locus_tag="VGB68_16770"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0050949.1"
                     /translation="MIKKFIFVLTLFGIIGAGLLSWSQFESVSAQASEEKAVRDVLTK
                     EAAAVEKGDLAALDKLWANDESVTVFESGHANYGWADYRNNHLAPELKEFKNTKYAFS
                     DLRIKVDGKTAWATFKYTIAADL"
     gene            707..1024
                     /locus_tag="VGB68_16775"
     CDS             707..1024
                     /locus_tag="VGB68_16775"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEY0050950.1"
                     /translation="MKKNNKESKSAAHGGIEVKHDRQLPRLSRIEGQVRGLQKMIDAE
                     RNCLDIVHQISAIINALRRVQTDMMRDHLTVLGEAIVAENLSPQMRREMADELAALFK
                     QLS"
     gene            complement(1138..>4248)
                     /locus_tag="VGB68_16780"
     CDS             complement(1138..>4248)
                     /locus_tag="VGB68_16780"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0050951.1"
                     /translation="NGLPGVERVRSQSGIGLSIVYVEFGWGTDVYRNRQLVAEKITEA
                     REQLPEGVSPFLAPISSIMGEIMLISVTSRDGKTNPLDVRTLADWTVRPRLLTIAGVS
                     QVIPIGGGVKQYQALVSPEKLAQFGITIEDVTTALEKSNANSTGGFVDAQSQEYLVRN
                     LGRFYTIDELKQTVVAYRSNTPIRLGDVASVEFGARIKRGDAGSNGQPAVIMSVQKQP
                     GASTIELTEKVDQAVRELQTTLPPDIEINTRLFRQANFIEASISNVIEALRDGAILVA
                     IVLFLFLLNFRTTAITLTAIPLSFVVTFLIFYAFGVTINTMTLGGLAVAIGELVDDAI
                     VDIENIFRRLRENYALAVPRPALEVIYHASLEIRSSIVYATIIVALVFLPLFMLTGVE
                     GRLLAPLGLAYITSLVASLFISLTVTPVLASYLIPQLFKNKSGRKSYWSRWFENLKAR
                     FRRNEDRENVETDSTVSADSSSEEDSFIVRWLKKRDERLLHWTLRHPYKVIAGAAILF
                     FLTMATLPFVGTSFLPDFNEGTLTINVLAQPGTSLTESNRIGTIAEKLILEVPEVVST
                     GRRTGRAELDEHAEGVHYTEIDVDLKESERTRAEILADIREHLAVIPGVSVNVGQPIS
                     HRLDHLQSGVRAQIAVKLFGDDLGVLRSKAEEIRNAMQTVEGATDVQIEKQVLIPQIR
                     FNVDRARAAQYGLQPGEITETLETALNGRRVSEAIEGARRYDVVVRFDDASRNNLDSL
                     RSVTVDTPQGTQIPVSAVATIENLPGPNQILRENTQRRIVISANTSGRDLGSVVQDIE
                     QRIGAQVTLPQGYFVEYGGQFQASQEATRTLSVLSLFSLVAIFFLLIKALGDWRAALQ
                     VMVNIPLALIGAVIALLLTGGVFSIATLVGFISLVGITSRNGIMMISHYLHLMREEGE
                     DFTEAMIIRGSLERLVPVMMTALTAGLSLIPFILAADAPGKEILHPLAVVVLGGILTS
                     TLLDQLVTPAVFYKFGRKISERVINERGRAGNGKNEWGEAETDSDRDEDIPPFSEKLP
                     KPVLGD"
BASE COUNT          990 a         1198 c         1114 g          946 t
ORIGIN      
        1 caggtctgcg gcgattgtgt atttaaaggt cgcccaggcg gttttgccgt caaccttaat
       61 tcgcaaatcg gaaaaggcgt atttcgtgtt tttaaactct ttcagttcgg gcgccaggtg
      121 attgttgcgg taatccgccc agccgtaatt ggcgtgcccg ctttcaaaaa ccgtcactga
      181 ctcatcattc gcccagagct tgtcgagcgc ggctaaatcg cctttttcga ctgctgccgc
      241 ttcttttgtc aaaacatcgc gcacggcttt ttcttctgaa gcttgtgccg aaacgctctc
      301 aaactgactc cagctcaaca aacctgcgcc gataatgccg aaaagcgtta aaacgaaaat
      361 gaatttttta atcattaaag attctcccta ttttaaatcc caaaaccgaa tgttacttcc
      421 gtgtcgattc gcattgcgca aacccgggtc tgtttgtttg tccatttaaa acgccctttg
      481 cctgcttaat tcaaaattcc gagccgcatt cataaaaccc cttttctaca tctaaagtcg
      541 ccgagccttt acaaaagccg cgtctttggc gaagattttt agaaagcgac ggagcgaatt
      601 ggcgattcgt gccccgccgc tcgctttcga cggtatttta gctgctaact aaaacgaccg
      661 tctgttctcg ccgaacgcct taaaccaagg taaggagtca gcgaaattga aaaaaaacaa
      721 caaagaatca aaaagtgcgg cgcacggcgg cattgaagtc aaacacgacc ggcagcttcc
      781 ccgcttgagc cgcatcgaag gtcaggtccg gggcttgcaa aagatgattg acgcagagcg
      841 caattgtctc gacatcgtgc atcagatttc agctattatt aacgctctgc ggcgcgtcca
      901 gaccgatatg atgcgtgacc acctgaccgt cctcggcgaa gccatcgtcg ccgaaaatct
      961 ctccccgcaa atgaggcgcg aaatggcgga cgaactcgcc gcgctgttca aacagttgtc
     1021 ttaaaatttg gaagaatctt acgtactcgt aattttcaaa gaaccgtttt tatctgcggg
     1081 cgggcggcga cgcgtctggc taaaaaccaa cgcccccggc gctatcccgc aaaatttcta
     1141 atcgcctaaa actggctttg gcagtttttc cgaaaacggc ggaatatcct catcccggtc
     1201 gctgtcggtt tccgcttcgc cccattcatt tttgccgttt ccggctcgcc cgcgctcatt
     1261 gattacccgt tcggaaatct ttcgcccgaa tttgtaaaac acggcgggcg tgacgagctg
     1321 gtcgagcagc gtcgaagtta aaatgccgcc gagaacgacg accgcgagcg ggtgcagaat
     1381 ctccttgccg ggtgcgtccg ccgcgagtat gaacggaatc agagaaagac ctgcggtcag
     1441 agcggtcatc attactggca ctaatctttc caagcttccg cgaataatca tcgcttcggt
     1501 aaaatcctcg ccttcttcgc gcattaagtg cagataatga gaaatcatca tgatgccgtt
     1561 gcgcgaggtg atgccgacga gcgagataaa accgacgagc gtcgcaatcg agaacacgcc
     1621 gcccgtcagc agcagcgcaa taacggcgcc gatcaaagcg agcgggatat tgaccatcac
     1681 ttgcagcgcc gcgcgccagt cgccgagagc cttgattaaa agaaagaaaa tggcgacgag
     1741 cgagaaaagc gaaagcaccg aaagcgtgcg ggtcgcttcc tgcgacgcct ggaactgacc
     1801 gccgtattcg acgaaatagc cttgcggcag cgttacctga gcgccgatgc gctgctctat
     1861 atcctgcacg accgagccta aatcccgccc gctcgtattt gccgaaataa caatccggcg
     1921 ctgcgtgttt tctcttaaaa tctgattcgg acccggcaga ttttcgattg tcgcaaccgc
     1981 cgaaaccgga atctgcgtcc cctgcggcgt gtcaacggtc acgctgcgca gagagtccag
     2041 attgttccgc gaagcgtcgt caaagcgcac gacaacgtcg taacggcgcg ctccttctat
     2101 cgcctcggaa actcttcgcc cgttcaaagc cgtttccagc gtttcggtaa tctcgcccgg
     2161 ctgtaagccg tactgcgccg cgcgggcgcg gtcaacgtta aaccttattt gcggaatcag
     2221 aacctgcttt tcaatctgca cgtcggtcgc gccttcgacc gtctgcatgg cgttgcggat
     2281 ttcttccgcc ttcgagcgta agacacccaa atcgtcgccg aacagcttaa cggcaatctg
     2341 cgcgcggacg cccgactgca aatggtcgag gcggtgcgaa atcggctgcc cgacgttgac
     2401 ggaaacgccc ggtataactg ccagatgctc acgaatgtcc gccagaattt ctgcgcgcgt
     2461 gcgctcggat tctttcaggt caacgtcgat ttccgtgtag tgaacgccct cggcgtgctc
     2521 gtcgagttcg gcgcgtcccg tgcgccgccc ggtcgagacg acttccggca cttccaaaat
     2581 caatttttcg gcaatcgtcc cgatgcggtt cgattcagtc agagacgtgc cgggctgcgc
     2641 cagcacattt atcgtcagcg tcccttcgtt aaaatccggc aaaaaggaag ttccgacaaa
     2701 tggaagcgtc gccatcgtca aaaagaataa aatggcggct ccggcgatta ctttatatgg
     2761 gtggcgcagc gtccagtgca gcagacgctc gtcacgcttt ttcagccagc gcacgatgaa
     2821 gctgtcttct tcggaggaag agtctgcgct gacggtactg tccgtctcga cgttttcccg
     2881 gtcttcattt ctcctgaatc tggctttcaa attttcaaac cagcgcgacc aataagattt
     2941 tcgtccgctt ttgttcttaa aaagctgcgg tatcagataa gaagccagaa cgggcgtcac
     3001 cgtcaggctg ataaaaagcg acgcgaccag ggacgtgata taggctagtc ccaaaggcgc
     3061 gagaagcctg ccttcgacac cggtcagcat aaaaagcggc aaaaagacga gcgcgacgat
     3121 gattgtcgcg taaacaatcg aagagcggat ttctaaagat gcgtgataaa tgacttcgag
     3181 cgccgggcgc ggaacagcaa gcgcgtaatt ttcccggagc cggcggaaaa tgttttcgat
     3241 gtccacaatc gcgtcgtcca ccagctcgcc gatggcgact gccagtcctc cgagcgtcat
     3301 cgtgttgatg gtgacgccga aggcgtaaaa aatcaggaac gtgacgacga acgagagcgg
     3361 aatcgccgtc agcgtaatcg cggtcgtgcg aaagtttaag agaaataaaa acaggacgat
     3421 agcgactaag atagcgccgt cgcgcaaagc ttcgatgacg ttcgagatgg aagcttcgat
     3481 aaaattcgcc tgccggaaaa gtctggtgtt gatttcgatg tcgggcggca gagtggtttg
     3541 cagctcgcga actgcctggt caactttttc ggtcagttca atcgtcgaag cgccgggctg
     3601 cttctgcacg gacataatca cggcgggctg accgttgctg ccggcgtcgc cgcgcttgat
     3661 gcgcgcccca aactcgaccg atgcgacatc gccgagccta atcggcgtgt tgctgcggta
     3721 ggcgacgacc gtttgtttca gctcgtcaat cgtgtaaaac cgccccaaat ttctaaccag
     3781 atattcctga ctttgggcat cgacgaaacc gccggtcgag ttggcgttcg atttttcgag
     3841 cgcggtcgtc acatcttcaa tcgtgatgcc gaactgcgcg agcttttccg gtgaaacgag
     3901 cgcctgatac tgcttgactc ctccgccaat cggaatcacc tgcgagacgc ccgcaatggt
     3961 cagaaggcgc gggcgcaccg tccagtcggc taaagttcgc acatcgagcg gattcgtttt
     4021 cccgtcacga ctcgtgacgg agatgagcat aatctcgccc ataatcgagg aaatcggcgc
     4081 cagaaagggc gagactcctt cgggaagctg ctcgcgggct tcggtgattt tttcggcgac
     4141 gagctggcgg ttgcggtaaa cgtcagttcc ccagccgaac tcgacataga caatcgacag
     4201 cccgattccg ctctgcgaac ggacgcgctc gacgcccggc aaaccgtt
//
LOCUS       DASWNE010000860         3283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_411412, whole genome shotgun sequence.
ACCESSION   DASWNE010000860 DASWNE010000000
VERSION     DASWNE010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411412"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VGB68_16785"
     CDS             complement(<1..222)
                     /locus_tag="VGB68_16785"
                     /inference="COORDINATES: protein motif:HMM:NF015418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050952.1"
                     /translation="MIPISEALEIIKREAFALEAEAVDLEKSVGRVLAEEIRADTDLP
                     PFDRSQMDGFAVRVEDTKNAPAKLKIIGEA"
     gene            complement(445..1803)
                     /locus_tag="VGB68_16790"
     CDS             complement(445..1803)
                     /locus_tag="VGB68_16790"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEY0050953.1"
                     /translation="MKTINKILFSFFLIFCLLNGNFVSAQNKSNEKLPPLPKLLSQRE
                     QLRVREEWLKKRFDTVLLPMMRKHNVSMWIVTNEEFHADPVTEYIAPPIPYVGRRDFF
                     ILADNGRTLDKIAVVRYAEEHLRNFYTVLNPPGNKIGETLKQIVSERNPKTIALNMGG
                     NRGQSDGITRDAYKFLADALGKDYESRFFSGGKFITDYLDTRIPEELEHYRQAVLLTD
                     ILTRRAFSNEVITPGKTTVGDVRWWLLQQVNNFGLTTWFQPDLRVQRLAKPNENSTQF
                     LEVTSENVRLERGDLIHVDFGLNYMGLSTDWQKHAYILKKGERDAPQGLKDALKNTNR
                     LQDALFAVARAGMTGPEVYAATMAQMKKQNIQAMIYSHPLGTHGHGLGAAIDFRRGIT
                     AADAERLRLGSYTSIELNTSTKVPEWNNQMVTIMAEDDAFMTEKGYEFFRPRQTEFYL
                     IR"
     gene            1923..3149
                     /locus_tag="VGB68_16795"
     CDS             1923..3149
                     /locus_tag="VGB68_16795"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY0050954.1"
                     /translation="MIDFELTEEHIALQKTVREFCRQEVAPYIKEWDEKSHFERSILG
                     KMADLGILGVCIPEQYGGAGFDYVSLGLVCEELEACDTFLRVIMSVHVGLNSMSVFSW
                     GTEEQKQKYLAPQARGEKISTFGLTEPNAGSDVIGLRSSARRDGDDWILNGEKMWISL
                     GDVADHFLFFCWTDEEKRKKRDHTGMSCFIVERTMPGFSSGTIHGKLGIKAGNTGYFS
                     LQDVRVPQANMLGQEGEGFKIAMFSLENGRYTVASGATGVIRASRDASVAYANTRETA
                     GVKIGEHQLVKQKIAEMEADYQMSHLLWLKTGYLKNHGLPSAKAASLAKWQATVRSEK
                     AASMAIEVHGANGYTNDYPVERYLRNCKAAVIYEGTRDIHTIMQADWALGLKREKQAR
                     VTLPPYKSNEAKDATA"
BASE COUNT          840 a          803 c          827 g          813 t
ORIGIN      
        1 cgcctcgccg atgattttca atttcgcggg cgcgtttttc gtgtcttcaa cccgcacggc
       61 aaaaccgtcc atctgcgagc ggtcaaacgg cggtaaatcc gtatctgcgc gaatttcctc
      121 agccaaaacc cgaccgaccg atttttccaa atcgaccgct tcggcttcga gcgcgaaagc
      181 ttcgcgtttg ataatttcta atgcttcgga aatcggaatc ataaatttta gccacagagg
      241 gcacagagaa cacagagaga atttaaccac agatttacac agataaacgc ggatttgaaa
      301 caggatggac aagatataca ggataaaagc ttttaaatga caaaattctg aataaagctc
      361 agatgttgat taaaaaacaa tttatcctga atatcctgtc catcttgtta aaatttccct
      421 ctgtgtcctc tgtggctaaa tttcttaacg aatcaaataa aactcggtct ggcgcgggcg
      481 gaaaaactcg tatcctttct ccgtcataaa agcgtcgtct tccgccataa tcgtcaccat
      541 ctgattgttc cattcgggca ctttggtcga agtgttcagc tcaatcgaag tgtaagagcc
      601 gagccgcagg cgttcggcgt cggcggcggt tatcccgcgc cggaaatcaa tcgccgcacc
      661 gagcccgtgt ccgtgcgtgc cgagcgggtg cgaataaatc atcgcctgga tattttgttt
      721 tttcatctgc gccatcgtcg ccgcgtaaac ttctggtccg gtcattccgg cgcgggcgac
      781 ggcgaataaa gcgtcctgca aacggttcgt gtttttcagg gcgtctttca gcccctgcgg
      841 cgcgtcgcgc tcgccttttt tcaaaatata cgcgtgtttc tgccagtcgg tcgaaagccc
      901 catataattc aagccgaaat cgacgtgaat caaatcgccg cgctccagac ggacgttttc
      961 cgacgtcact tcgagaaact gcgtcgaatt ttcgttcggt ttcgccagcc tctgaacgcg
     1021 caaatccggc tgaaaccagg tggtcagacc gaaattgtta acctgctgca aaagccacca
     1081 gcgaacgtcg ccgacagtcg ttttgccggg cgttataact tcgttggaaa acgcgcggcg
     1141 cgtcaaaata tcggtcagta aaaccgcctg ccggtaatgt tccagctctt cgggaatacg
     1201 cgtgtccaga tagtcggtga taaattttcc gccggaaaaa aagcgcgatt cgtaatcttt
     1261 gccgagcgcg tcggctaaaa atttgtaagc gtcgcgcgtg atgccgtcgg attgcccgcg
     1321 attgccgccc atatttaaag cgatggtttt cggatttctc tcgctgacga tttgcttcag
     1381 agtctctccg attttattgc ccggcggatt caaaaccgtg taaaaatttc tcagatgttc
     1441 ttcggcgtag cgcacgacgg cgattttatc gagcgtccgc ccgttgtcgg cgagaataaa
     1501 aaaatcgcgt ctgccgacat acggaatcgg cggcgcgatg tattccgtca ccggatcggc
     1561 gtgaaattcc tcgttggtga caatccacat cgaaacgttg tgtttgcgca tcatcggcaa
     1621 aagcaccgtg tcgaaacgct ttttaagcca ttcttcgcgg actctcaact gctcgcgctg
     1681 acttaaaagc ttcggcagcg gcggcagctt ctcgttcgat ttattctgtg cagaaacgaa
     1741 atttccgttc agcagacaga aaatcagaaa gaaagaaaac agaattttat tgattgtctt
     1801 catatataaa tattgttttt atttccggct tttagcgcaa atttttacaa aatgataaac
     1861 tgatttttgt ttcgactgcc ttaatttacc ataaaaattt atttgagagg attttataaa
     1921 ccgtgattga ttttgaattg accgaagaac atatcgcttt gcaaaaaacc gtccgcgaat
     1981 tctgccggca ggaagtcgcg ccgtatatca aggaatggga cgaaaaatcg catttcgagc
     2041 gctcgattct gggaaaaatg gctgatttgg gaatcctggg cgtatgcatt cccgaacaat
     2101 acggcggcgc cggattcgat tacgtttcgc tcggcttggt gtgcgaagaa ctcgaagcct
     2161 gcgacacgtt tttgcgcgtg attatgtcgg ttcacgtcgg cttgaattcg atgagcgttt
     2221 tcagctgggg aaccgaagaa cagaagcaga aatatctcgc gccgcaggcg agaggcgaaa
     2281 aaatctcgac cttcggtctg accgagccga acgcgggttc cgacgttatc ggcttgcgct
     2341 catcagccag acgcgacggc gacgactgga ttttgaacgg cgaaaaaatg tggataagct
     2401 tgggcgacgt cgccgaccat tttcttttct tctgctggac ggacgaagaa aaacgaaaaa
     2461 aacgcgacca cacgggaatg agctgtttta tcgtcgagcg cacgatgccg ggattttctt
     2521 ccggcacgat tcacggcaaa ttaggaatta aagcgggaaa caccggatat ttttcacttc
     2581 aggatgtgcg cgtgccgcag gcgaatatgc tcggacagga aggcgaaggt tttaaaatcg
     2641 cgatgttttc attggaaaac ggacgctaca cggtagcttc gggcgcgacg ggcgtgattc
     2701 gcgcttcgcg tgacgcttcg gtcgcgtatg cgaacacgcg cgagacggcg ggcgtcaaaa
     2761 tcggcgagca tcagctcgtc aaacaaaaaa tcgccgaaat ggaagccgat tatcaaatgt
     2821 cgcatctttt gtggctcaaa acgggttatc tgaaaaacca tggcttgccg agcgcgaaag
     2881 ccgcctcgct ggcgaaatgg caggcgaccg tcagaagcga aaaagcggct tcgatggcaa
     2941 ttgaagttca cggcgcaaac ggttacacga acgattatcc ggtcgaacgc tatctgagaa
     3001 actgcaaagc tgccgttatc tacgagggaa cgcgcgacat tcacacgatt atgcaggcgg
     3061 attgggcttt gggcttgaaa cgcgaaaaac aagcccgcgt gacgctgccg ccgtataaat
     3121 cgaatgaagc gaaagacgcg acggcataag tagggaaacc cgaaaatgca aagcgcaaaa
     3181 tattaggctt tggaattcag gattcaggat taaatcaact ctgccgtttt gcgctttgca
     3241 ttttcgtgtt ttcgaccttt ttatcttctt tctcatccaa taa
//
LOCUS       DASWNE010000861         6191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_624691, whole genome shotgun sequence.
ACCESSION   DASWNE010000861 DASWNE010000000
VERSION     DASWNE010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6191
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624691"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(520..3342)
                     /locus_tag="VGB68_16800"
     CDS             complement(520..3342)
                     /locus_tag="VGB68_16800"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0050955.1"
                     /translation="MKIAKSYLIVICLLVSTISVFAQKPIEKTTFKVDYEKFTLANGL
                     EVIFHVDRSDPVVAVALTAHVGSAREKAGRTGFAHLFEHLLFLESENLGKGGLDKMSA
                     RIGGSGANGSTSRDRTNYFQTVPKDALEKMLWAEADKLGWFINTVTEPVLAKEKQVVK
                     NEKRQSYDNSPYGHTSYVIDKALYPQDHPYNWQVIGSLDDLQNATLADVKEFFRRWYV
                     TNNVTLVVAGDFDTAQAKKWVEKYFSEIKRGEDIKPLAKRPGAVKETVKFYHEDNFAK
                     LPELTMAWIAAEQYHPDSYALDVLAQYLAQGKKAPFYQVLVENKKLAPSVRISNDTSE
                     LAGQFELAVRAFEDKDLDEVAKAIDEAFAKFEKEGISEKDLSRIKAGQETQFYNSLSS
                     VLGKGFQLAQYNIFADDPGFIEKDIKNILAVTPADVVRVYQKYIKNKNYAATSFVPKG
                     KPALALENSKKAEIAEEKIVTGAEDDVDPNITATYEKTPSSFNRSVEPPYGDAPEVKI
                     PAIWEQKLSNGLRILGIENAEVPLVQFEIVIDGGQFLENINKVGVSNLTARMMTQGTL
                     RKTPQELEEAIQQLGATINVIAGTEDVRISVNTLARNYEPTLALVEEILFEPRWDAKE
                     FDLVKQSTISQIRQQEANPNAVAQNNFNLLIYGKDNIRSRNILGTIESVNSITLDDLK
                     AFYNKNFSPSVARMHVVGALDKASITKSLNGINKNWKSKKVEIPEYKTPSAPAKSQIY
                     FYDVPDAKQSVIRFGYPALAQTDKDFYPATIMNYILGGGSFASQLTQQLRETKGYTYG
                     ISSNFSGTKSPGAFAIASGVRSNVTLESAQLIKSILQDYPKNYSENDLETTKSFLIKS
                     NARAFETAGAKLNMLENISKYGWRYDYVKDREQIVKAMSVQQIKDLSQKYLNADKMIW
                     LVVGDAKTQLPRLKELGFGDPVLLNK"
     gene            complement(3574..>6191)
                     /locus_tag="VGB68_16805"
     CDS             complement(3574..>6191)
                     /locus_tag="VGB68_16805"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0050956.1"
                     /translation="GFFAAGGIKHNDRVILFSHNMPEWGLSYFGILKTGATVIPIDPA
                     SSVDEITAFAKAGEASAIVLSPKLASENPDLAEKLLEAGIEIPLWTFEEVFELHSETE
                     EAKRLALLPNKVLSNSVASLIFTSGTTGTPKAVMLSHKNFVNMISMLSSVLDMDITDG
                     VLSVLPMHHTFEFSAGFLTPFSNGTQITYLDELSSEELTRAIENGHVTGMVGVPALWE
                     MLHRRIKTRLRERGDWFADLADNVIEFNAWVRDNTPFNLGPIIFFPIHQGMGGRMRYL
                     ISGGSALSEKVQKDLHGLGFTVLEGYGLTESSPVLTVARPGNKLLRGSVGKPLPGVEV
                     KINNPDENGVGEVLARGQNIMLGYYNNEEATEAVLHDRWLRTGDLGKLDEDGNLYIVG
                     RSKDVIIDSNGKNIYPDEIEDLYGKSPFIKELSIVGLPDEDGGEKIAALVVPDYEHDI
                     SLSRSDTNKKVEEHFREISASLAFFKRVKVLHLTPFELPRTATRKVKRPEVVQMLQNL
                     EDKARRKTKAVAESKGDDNVLWLRKIVGSVSNRPLSDVALDDKLADLGFDSLMFVELQ
                     AAVEDAGGRVPSPDNLNEVQTVRELLTAVQRVDKSKKIADEPRVEEKKEDELFIPSIV
                     RRVGNQLVGLAQNTLYSNVLQTSVEGESNVPPHTNFIVAANHSSHLDMGLVKYALGDD
                     VAEQTVAVAAADYWFDTKYKRAYMNNFTTLVPIERTGSLRQSLRHVTQILNEGYNTLI
                     FPEGGRQISGIIAEFKPIIGYLSLNNKVGILPVYLWGTFEAFPKGATIPKAQSIGAKI
                     GAKIGRFLEYEELAEMTKGVPNTEAYRLIAARVQHEIENMRDDRRDKFDVAAVRKKWR
                     AERRKTRKQQPVIDE"
BASE COUNT         1375 a         1591 c         1529 g         1696 t
ORIGIN      
        1 caaaccgaat ttacaaattc ttcaccgccg gttaagcgat tgttcggaaa gtttttttat
       61 tgttaatcga cttttacaat taaagacaag agttcggcgt ctgatactcg catatttcac
      121 gagggtcatt ctgtttacaa caaactttaa cgcagcagga cgcatcctgg acgcagtaat
      181 cgccgtagac cagacagtaa ttcgcctccg caatattttg ttcggcaaaa agaaaagcgc
      241 aagaggcgag cataaccggc agagcaaaca ttctaagttt cttcataaat ttctccttta
      301 gctttttcgg caggtttata aattccgttt aatttaagct ttatcgatta cataaagttt
      361 tgcctgtaat caattttgca aaaatcgtct tgtaatgcag attcacatac actatagact
      421 tatgattacc aattgtaaat aaaaagccgg taaactctta gctttctctg cggaggaaaa
      481 agcaagattt taccggcttt tttatcttct ctcgcccgtc tatttattca gcaaaacggg
      541 gtcgccgaat ccgagctctt tcagacgcgg caattgggtt ttggcatcgc cgacgaccag
      601 ccagatcatt ttatcggcgt tcagatattt ctgcgataaa tcttttattt gctgcacgct
      661 catagctttg acgatttgct cgcggtcttt cacgtaatcg tatctccagc cgtatttact
      721 gatattttcc agcatattca gctttgctcc ggcggtttca aaggcgcgcg cgttgctttt
      781 aatcagaaag cttttcgtgg tttccaaatc attttccgag taatttttcg ggtaatcctg
      841 caaaatgctt ttaattaact gagccgattc cagagtcacg ttgctgcgga cgccgctggc
      901 gatggcaaac gcgccggggc ttttcgtgcc gctgaagttc gagctgatgc cgtatgtgta
      961 gccctttgtc tcgcgcagct gctgggtgag ctgcgaggcg aaactgccgc cgccgagtat
     1021 gtagttcata atcgtcgccg ggtaaaagtc cttatcggtt tgagccagcg ccgggtaacc
     1081 gaaccgtatg acggattgtt tcgcgtcggg aacgtcgtag aaatagattt gcgattttgc
     1141 cggcgcagac ggcgttttat attcgggaat ctcgactttt ttacttttcc agtttttatt
     1201 gattccgttt aatgactttg taatcgacgc tttatccaaa gctccgacga cgtgcatacg
     1261 cgcgaccgac ggcgagaagt ttttattata aaaagccttt aaatcgtcga gcgtgatgga
     1321 attgaccgat tcgatagtgc ctaaaatatt gcgcgagcga atgttgtctt tgccgtaaat
     1381 caaaaggttg aaattatttt gggcgaccgc gttgggattg gcttcctgct ggcggatttg
     1441 gctaatcgtg ctttgtttga ccaaatcgaa ttctttggcg tcccagcgcg gctcgaatag
     1501 aatttcttcg accagcgcca gcgtcggctc gtaatttctc gccagcgtgt taaccgaaat
     1561 tctgacatct tcggttccgg cgatgacgtt aatcgtcgcg ccgagctgct gaatcgcttc
     1621 ttcaagctcc tgcggagttt ttctgagcgt cccctgcgtc atcatccgcg ccgtaagatt
     1681 cgaaacgccg actttattga tattttccag gaactgcccg ccgtcgatga cgatttcaaa
     1741 ctgcacgagc gggacttccg cgttttcgat gcctaaaatg cgcaagccat tggaaagttt
     1801 ctgttcccag attgccggaa ttttaacttc cggcgcgtcg ccgtaaggcg gctcgacgct
     1861 gcggttaaag ctcgaaggcg ttttttcgta agtcgccgtt atattcgggt cgacgtcgtc
     1921 ttccgcgccc gtcactattt tttcttcggc gatttcggct tttttcgaat tttccagcgc
     1981 cagcgccggt ttgcctttcg gcacgaagct cgtcgccgca tagtttttat tcttgatgta
     2041 tttttgataa acgcgcacga catcggcggg cgtgacggct agaatatttt taatatcctt
     2101 ttcgataaag ccggggtcgt cggcaaaaat attgtattgc gcgagttgaa atcccttgcc
     2161 gagaacgctc gaaagagaat tataaaactg cgtttcctgt ccggctttga tgcggctgag
     2221 gtctttttcc gaaatccctt ctttttcaaa tttggcgaaa gcctcgtcaa tcgcttttgc
     2281 cacttcatcg aggtctttat cttcaaaagc gcgcacggct aactcgaatt gaccggcaag
     2341 ctccgaggtg tcgttggaaa ttctcacgct cggcgcgagt tttttattct cgaccagaac
     2401 ctgataaaaa ggcgcttttt tgccttgcgc caaatattgc gccagaacgt cgagcgcgta
     2461 ggaatccgga tgatactgct cggcggcaat ccacgccata gtcagctcgg gcaattttgc
     2521 aaaattatct tcgtgataga atttgaccgt ttctttaacc gcgcccggtc ttttcgccag
     2581 cggcttgatg tcctcgccgc gttttatttc gctgaaatat ttttcgaccc atttttttgc
     2641 ctgcgccgta tcgaaatcgc ccgccacgac gagcgtcacg ttgttggtca cgtaccagcg
     2701 ccggaaaaat tccttcacgt cggcgagcgt cgcattctgc aaatcgtcga gcgagccgat
     2761 gacctgccag ttgtacggat ggtcctgcgg gtagagggct ttatcgatga catacgaagt
     2821 gtgaccgtac gggctgttgt cgtagctttg ccgcttctcg tttttgacga cctgtttttc
     2881 ctttgccaga accggttcgg tgacggtatt gataaaccag ccgagcttgt cggcttccgc
     2941 ccagagcatt ttttccagag cgtctttcgg aacggtctgg aaataattgg tgcggtcgcg
     3001 cgaagtcgaa ccgtttgcgc cggaaccgcc gatgcgggcg ctcattttat cgagtccgcc
     3061 ctttcccaga ttttccgatt cgagaaacaa aagatgctcg aaaaggtgtg caaatccggt
     3121 gcgccccgct ttctcgcgcg ccgagccgac gtgagccgtc agggcgacgg cgaccaccgg
     3181 gtcggaacgg tcgacgtgaa aaatcacttc caaaccgttc gccagagtaa atttttcgta
     3241 atcgacctta aacgtcgttt tttcaatcgg cttttgtgcg aaaacggaaa tcgtcgaaac
     3301 cagcaggcaa ataacgataa gataagattt tgcaattttc ataattattt taaggcggcg
     3361 agctgaaaag ccgcgctatt tcaaaatcga gaaacgagtt ctttgtaatt gatgttagta
     3421 cgaacgcact ttaatctttg gtttcaaaac tttgtcgaat ttattttcaa agccagagaa
     3481 ttcaccgcaa ctgaaagcgg taagaataat atgctctgaa tttaactcga acgataattt
     3541 gcggcaatta tttttaaatt gcgtttttca tttttattca tcaattaccg gctgctgttt
     3601 gcgcgtcttt ctgcgctcgg cgcgccattt tttgcgcacg gcggcgacgt cgaatttatc
     3661 gcgccggtcg tcgcgcatat tttcgatttc gtgctggacg cgggcggcaa tcaggcgata
     3721 ggcttccgtg ttcggaacgc ctttcgtcat ttccgccagc tcttcgtatt ccagaaagcg
     3781 cccgattttc gcgccgattt tcgcgccgat gctctgggct ttcggaatcg ttgcgccttt
     3841 cggaaacgct tcgaaagtgc cccacagata gacgggcagg attccgactt tattattcag
     3901 agacagatag ccgattatcg gcttgaattc ggcgatgatg cccgaaatct gccgcccgcc
     3961 ttcggggaaa atcagagtgt tgtagccttc gtttaaaatc tgcgtcacgt ggcgcaaaga
     4021 ttggcgcagg ctgccggttc gctcgatcgg gacgagcgtc gtgaaattgt tcatatacgc
     4081 gcgtttatat ttcgtgtcga accagtaatc agccgccgct accgcgaccg tttgttccgc
     4141 cacgtcgtcg ccgagcgcgt attttaccaa gcccatatcg agatgcgacg agtgatttgc
     4201 cgcgacgatg aaattcgtgt gcggcggcac gttcgattcg ccttcgacgc tcgtttgcag
     4261 aacgttgctg taaagcgtgt tctgtgccag accgaccagt tgatttccga cgcggcggac
     4321 aatcgaggga ataaaaagct cgtcttcctt tttctcttcg actcgcggct cgtcggcgat
     4381 tttcttcgat ttatcgacgc gctgaaccgc cgtcagaagc tcgcggacgg tctgcacttc
     4441 gttcagatta tcgggcgacg gaacgcgccc gcccgcatct tcgaccgccg cctgcaattc
     4501 gacaaacatc agtgaatcaa agcccaaatc ggcgagctta tcgtccagcg ccacgtccga
     4561 aagcgggcga ttggagacgc ttccgacgat tttgcggagc cacaaaacat tgtcgtcgcc
     4621 tttggattcg gcaacggctt tggttttccg gcgcgcttta tcttcgagat tttgcagcat
     4681 ctggacgact tcgggacgct tgaccttgcg cgtcgccgtg cgcggcagtt caaacggcgt
     4741 caggtgcaag accttgacgc gcttgaaaaa cgccagagaa gcggaaattt cgcgaaaatg
     4801 ttcttcgact tttttattcg tatcgcttcg gcttaaggaa atatcgtgct cgtaatcggg
     4861 cacgaccaga gccgcgattt tttcgccgcc gtcttcgtcc ggcaagccga cgattgacaa
     4921 ttccttgata aaaggagatt ttccgtataa atcctcgatt tcgtccgggt aaatattttt
     4981 gccgttcgag tcgataataa cgtctttcga gcgcccgacg atataaagat ttccgtcttc
     5041 gtcgagtttc cccaaatcgc cggtgcgcag ccaacggtcg tgcaaaacgg cttcggtcgc
     5101 ttcctcgttg ttgtaatagc cgagcataat attctgcccg cgcgccagaa cttcgccgac
     5161 gccgttttcg tccgggttgt tgattttgac ttccacgccc ggaagcggtt tgccgacgct
     5221 tccgcgcagc agtttattgc cgggacgcgc gacggtcaga acgggcgaag attcggtcaa
     5281 accgtagcct tcgagaaccg tgaaaccgag tccgtgcaaa tctttctgga ctttttcgct
     5341 taaagccgag ccgcccgaaa tcagataacg catccgtccg cccattccct gatgaatcgg
     5401 gaaaaagatg attggaccca ggttaaaagg cgtgttgtcg cgcacccagg cgttaaattc
     5461 gataacgtta tcggctaaat cggcaaacca gtcgccgcgc tcgcgaaggc gggtcttgat
     5521 tcgccggtgc agcatttccc agagcgccgg cacgccgacc attcccgtca cgtgaccgtt
     5581 ttcgatggcg cgcgtcagct cttcgctgct caattcgtcg agatacgtaa tctgcgtccc
     5641 gttggaaaaa ggcgtcagaa aaccggcgga aaattcaaac gtgtgatgca tcggcagaac
     5701 cgacaaaacg ccgtcggtga tgtccatatc cagaacgctc gacagcatcg aaatcatatt
     5761 gacgaaattt ttgtgcgaga gcatcacggc tttcggcgtt ccggtcgtcc cggaagtgaa
     5821 aatcaaagac gccaccgaat tcgataaaac tttgttcggc aaaagcgcga gccgtttggc
     5881 ttcttccgtt tcgctgtgca gctcgaaaac ttcttcaaac gtccagagcg gaatctcgat
     5941 gcccgcttcg agcaattttt cggctaaatc cggattttcg ctcgccagct tcggcgacaa
     6001 aacaatcgcc gaagcctcgc ccgcctttgc aaaagcggtg atttcgtcga ccgagctcgc
     6061 cgggtcaatc ggaatcacgg tcgcgccggt ttttaaaatc ccgaaatacg acaagcccca
     6121 ttctggcata ttatgcgaaa acaaaatcac gcggtcgtta tgcttgatgc cgccggcggc
     6181 aaaaaatccg g
//
LOCUS       DASWNE010000862         5752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_522164, whole genome shotgun sequence.
ACCESSION   DASWNE010000862 DASWNE010000000
VERSION     DASWNE010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5752
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522164"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..1598
                     /gene="lpdA"
                     /locus_tag="VGB68_16810"
     CDS             189..1598
                     /gene="lpdA"
                     /locus_tag="VGB68_16810"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEY0050957.1"
                     /translation="MAEQFDVTIIGSGPGGYVAAVRGAQIGLKVALVEKEKDARLGGT
                     CGLRGCIPTKALLQAAHLYEKAEHFADWGIKVENLGFDWAQVQKRKSDVVAKNSAGVS
                     YLMKKHKITVFNGFGKIAGKGKVEVELDGGKKETIDTKNIIIATGSVVRPIPGFEVDG
                     KQVVNSDQILELDRVPKSMLVMGSGAVGVEFASVYSRFGCETTIVELMERIVPVEDAE
                     VSKELARAFKKRGIKIETGIKLDKLQKSKTGVKATGKDSKGKDVAFEAEMFLVAVGRM
                     PYLEKLGLENTKVVVNPRGTIKVNGYCETDEPNVYAIGDVIDTAWLAHLASKEGILVV
                     EKIAGKKVEPINHRLVPNCTYCDPEVASVGLTEAKAKEEGYDVKVGKFPFSASGKARI
                     LGETDGFVKIVSEKKYDEVLGVHIIGPHATELLAEACVAMQLETTADELGRTIHAHPT
                     VSESVMEAAEGVHDLTIHI"
     gene            complement(1767..2708)
                     /locus_tag="VGB68_16815"
     CDS             complement(1767..2708)
                     /locus_tag="VGB68_16815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0050958.1"
                     /translation="MKKEKSEFYKVFQEIIRENGKHNPKTVAEEAELIGISAAQLSRI
                     KSGNSSLTKRVIDLIAQTFGINEESKRLIRERLEFSKAQDVLKESEPTVKTALEEFHN
                     RFFHTNRGEKRIICGAFFDIAQSTQKGRNPEYADASADVIRNGLSFACFQPFGPRENI
                     LKLVKKAVDAKNQELIAIWNYIYRLADSVHEGLEKTKESLKKEETLGQIVLYEAANVP
                     SLTNCNLHSRLYYGERLSGDNKIQIVQLLLGKTGNSEYTEHYIECINESAFQSVVAEQ
                     FSPVLNYWRVTGKLPTIEEQIHKTLGKNTICPWKIYT"
     gene            2927..3796
                     /locus_tag="VGB68_16820"
     CDS             2927..3796
                     /locus_tag="VGB68_16820"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY0050959.1"
                     /translation="MRNKESQTFSHIFVGVLVHLIAIGIISILSYFAFNLILPFLNEP
                     LDWGYLGVSVIAASVISYVIFVFIYSRITDRTKLKRNYGFHLISFLLILSLNASISAT
                     AIYVSKMSREKIEKAEMNVSDILNKCKDDFNIKLRSYSLRCAAVYYDQKGDKLRMPKI
                     GCSGQNCNSALAGNNYTVNKKGIDETQAQACLGSGIFGYVFKNHEPVNELNLQKPQNS
                     KICYKPVENMDFEQDISILCVPIDYQQKVKVGVVCVSSPKQTKFTEEKINEMKNFANQ
                     LSESINVLSEQYR"
     gene            3917..4684
                     /locus_tag="VGB68_16825"
     CDS             3917..4684
                     /locus_tag="VGB68_16825"
                     /inference="COORDINATES: protein motif:HMM:NF042851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HEY0050960.1"
                     /translation="MNLRTVKVMRYVTPLREGGSLPAIVEADDDGLYVLKFRGAGQGV
                     KALIAELVAGEIGRALGLRLPEIVFVELDARLARTEPDPEIQDLIKASDGLNLALDYL
                     PGSITFDTLIEKPAAALASKIVWFDAFAANVDRTPRNANLLWWHKNLWLIDHGAALFY
                     HHNMEAFAANSRNPFRQIKDHMLLPFADEIEKADAEASGRLTPKVLREIVNLIPDQWL
                     GEAEKERAGYLEFLTARLEAPREFVKEAVDARRNLHV"
     gene            4659..5045
                     /locus_tag="VGB68_16830"
     CDS             4659..5045
                     /locus_tag="VGB68_16830"
                     /inference="COORDINATES: protein motif:HMM:NF022681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HEY0050961.1"
                     /translation="MHEEICTFDYAVFRLVPRVEREEFFNVGVIVSCPAQKFLEARTG
                     LDEEKLKAFAPALDAKTIQRYLEIIPKICAGEAAAGVIGRLTPRERFYWLTAQRSTII
                     QASPVHTGFCADAGDVLRRLFDKMVG"
     gene            5086..>5752
                     /locus_tag="VGB68_16835"
     CDS             5086..>5752
                     /locus_tag="VGB68_16835"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0050962.1"
                     /translation="MATTQTSYPRSKIKILLLENISESAVEEFKESGYAEIRKISGAL
                     SEADLVREIKGVHILGIRSKTRITEKIIEKADRLLAIGAFCIGINQVNLKAATAKGVA
                     VFNAPYSNTRSVAELVIGLCVMLIRRITDKNMAAHRGVWQKDAAGSFELRGKTLGIIG
                     YGNIGSQVSVMAEALGMNVIYYDVLTKLPHGNAKQERDLRALLKKSNIVTLHVPSDAT
                     TRNM"
BASE COUNT         1588 a         1315 c         1344 g         1505 t
ORIGIN      
        1 aaagaagatg acctttgagt tttgagtctg taactttgat ttggcacaat cggcaaaggt
       61 cgaaaatcaa tcattcgtaa ttcgtaattt ttaattcgta attgtcctaa ctagcttgtc
      121 gccggataac ttcttataat aattctttcg cggtttttat aaaattttaa cagaagaggt
      181 ttaaaacatt ggcagaacaa tttgacgtaa cgattatcgg ttcgggaccc ggcggatacg
      241 tcgccgccgt tcgcggagcg cagataggtt taaaagtggc tttagtcgaa aaggaaaaag
      301 atgcgcgcct cggcggaacg tgcggcttgc gcggctgtat tccgaccaaa gcgcttttgc
      361 aggcggcgca cctgtatgaa aaagccgaac attttgccga ttggggcata aaggtcgaaa
      421 atctgggttt tgactgggcg caggtgcaga aacgcaaaag cgatgtcgtc gccaaaaact
      481 cggcgggcgt ttcctacctg atgaagaaac acaaaatcac cgttttcaac ggcttcggca
      541 aaattgcggg caaaggcaaa gtcgaagtcg agctcgacgg cggcaaaaaa gaaacgattg
      601 ataccaaaaa cattatcatt gcgacgggct cggtcgtgcg cccgattccg ggctttgaag
      661 tggacggcaa acaggtcgtt aattccgacc agattctgga actggaccgc gttccgaaat
      721 ctatgctggt gatgggttcg ggcgcggtcg gcgtcgaatt cgcttccgtc tattcgcgtt
      781 tcggctgcga gacgacgatc gtcgaactga tggagcgcat agttccggtc gaggacgcgg
      841 aagtttccaa agaactcgcc cgagccttta aaaaacgcgg catcaaaatc gaaaccggca
      901 tcaagctcga caagctgcaa aaatcgaaaa ccggcgtcaa agcgaccggc aaagattcaa
      961 aaggcaaaga cgtagccttc gaagccgaga tgtttctcgt cgccgtcggg cgtatgcctt
     1021 acctggaaaa gctcggcttg gaaaacacga aagtcgtcgt caacccgcgc ggcacaatca
     1081 aagtcaacgg gtactgcgaa accgacgagc cgaatgtcta cgccatcggc gacgtcatcg
     1141 acacggcgtg gctggcgcat ctcgcctcga aagaaggcat tctggtggtc gaaaaaatcg
     1201 cgggcaaaaa ggtcgagccg attaatcacc gcctcgtccc gaactgcacc tactgcgacc
     1261 cggaagtcgc ctcggtcggc ttgaccgaag ccaaagccaa agaagaaggc tacgatgtaa
     1321 aagtcggcaa attccctttc tcagcctccg gtaaagcgcg cattttgggc gaaaccgacg
     1381 gttttgtaaa aatcgtctcc gagaaaaaat acgacgaagt cctcggcgtc cacatcatcg
     1441 gaccgcacgc cacggaacta ttagccgaag cctgcgtcgc catgcaattg gaaactaccg
     1501 ccgacgaatt aggcagaaca atccacgcgc acccgaccgt cagcgaatcc gtgatggaag
     1561 cggcggaagg cgttcacgat ttgacgattc atatctaagt atggagagga tttttaactt
     1621 gtggaattta tttttgacga tttgacacaa taaattccac aagttttaaa gtccgctttc
     1681 caaataaaag atggttaaat taaaaagata agcgttttcg cttactaaaa gtaaaattag
     1741 ggtaatttcc aagaactaaa atactattat gtataaatct tccacggaca gatcgtattt
     1801 ttacctaaag ttttatgtat ttgttcttct atagttggca atttgcccgt gactctccaa
     1861 taattcaaaa ctggagaaaa ttgctctgca acaacagact gaaaagctga ctcattaata
     1921 cactcgatgt aatgctcggt atattcgcta tttccggttt ttcctaataa taactgtacg
     1981 atttgaattt tattatctcc agacaatcgc tcgccgtaat acagacggga atgaagatta
     2041 caatttgtta aggatggaac atttgcagcc tcataaagaa ctatttgccc taaggtttcc
     2101 tcttttttaa ggctctcctt agttttttct aatccctcgt gaacgctgtc ggctaatctg
     2161 taaatataat tccaaatcgc aattaactct tgatttttcg catctacagc tttttttacc
     2221 aatttcaaaa tattttctct gggtccgaac ggttgaaaac aagcaaagga aagtccattc
     2281 ctaatcacgt ccgcacttgc atcagcatat tccggatttc tgcccttttg cgtactttga
     2341 gcaatatcaa agaatgctcc gcaaattatt cttttttcgc cgcgattagt atggaaaaaa
     2401 cgattgtgaa attcttctaa cgcagttttc accgtcggct cagattcctt taatacatcc
     2461 tgagctttgg aaaattccaa tctttctctt attaatcttt tactttcttc atttatgccg
     2521 aatgtttgag ctattaaatc aataactctt tttgttaaag atgaattacc acttttaatt
     2581 ctactaagtt gtgcagccga aatgcctatt aactcagctt cctcagctac ggtcttagga
     2641 ttatgttttc cattttctcg gataatttcc tgaaaaactt tataaaattc tgatttttct
     2701 tttttcatcg acttatttat ataggaaact ttaaatcata ttctattgat ttttattgac
     2761 gtttcattca gaacacttta accctaaaat taaaataacg caaactattg tacaaggtac
     2821 gctatttgca taaaatacgt aaaattacgc aatccttctt ctttccccaa aattgcgcaa
     2881 cgacagcaat gcggcagcat cagcgaaaag actttaagag gtaaaaatga gaaacaaaga
     2941 atcacaaacg ttttcgcata tttttgtcgg cgttctcgtc catttaatag caatcggaat
     3001 catttccatt ttatcgtatt tcgccttcaa tctaattttg ccgttcttaa atgaaccgtt
     3061 ggactggggt tatctaggcg tttcagttat tgccgcaagt gtaattagtt acgtaatttt
     3121 cgttttcata tattcgcgta ttacggatag aacaaagttg aaaagaaact acgggtttca
     3181 tcttatatcc tttttactga ttttatcctt gaacgcctct atctcggcga cggcaatcta
     3241 tgtcagcaaa atgagcagag aaaaaatcga gaaagcggaa atgaatgtct ccgatattct
     3301 taacaaatgt aaagatgatt tcaatatcaa gcttcgatca tattcgctcc gatgcgcggc
     3361 ggtttactat gaccaaaagg gcgataaatt acgaatgcct aaaatcggat gcagcggaca
     3421 aaattgcaat tctgcgcttg ctggcaataa ctacacggtg aataaaaaag ggattgatga
     3481 aactcaagct caagcgtgtc tcggcagcgg catattcggc tatgtcttta agaatcatga
     3541 gcctgtaaat gaactcaatc tccaaaagcc gcagaatagt aaaatttgtt acaagcccgt
     3601 agaaaatatg gattttgaac aggatatatc aatactgtgt gttcctattg attatcagca
     3661 aaaagtcaaa gttggagtcg tatgtgtttc ctccccgaaa caaacaaaat ttaccgagga
     3721 aaaaataaat gaaatgaaaa actttgcaaa tcagttatca gaatcaataa atgttttgtc
     3781 agaacaatac cgatagacaa aatagagaaa ttgtttttct tgccgattag gttttatctt
     3841 atctgacaac aaaacggacg ggaacggttt attcgttctc gtccgttttg ttataatcac
     3901 actttttatt tcaaatatga atttgcgaac cgtgaaagtt atgcgatacg tgacgccttt
     3961 gcgcgaaggc ggttctctgc ccgcgattgt cgaagccgat gacgacgggc tttacgtgct
     4021 gaaatttcgc ggcgcaggac aaggcgtcaa ggctctgatt gcggaactgg tcgccggcga
     4081 aatcgggcgc gcgctcggac tgcgcctgcc ggaaatcgtt tttgtcgagc ttgacgcgcg
     4141 gctggcgcgg actgagcccg acccggaaat tcaggatttg attaaagcga gcgacggatt
     4201 aaatttagcg ctcgattacc tgcccggctc gattaccttc gacacgctga ttgaaaagcc
     4261 tgcggcggcg ctggcttcaa aaatcgtctg gttcgacgct ttcgcggcga acgtcgaccg
     4321 cacgccgcgc aacgccaatc tgctctggtg gcacaaaaat ctgtggctga tcgaccacgg
     4381 cgcggcgctt ttttatcacc acaatatgga agcgttcgcg gcgaacagcc gcaatccttt
     4441 ccggcagata aaagaccata tgctgctgcc gtttgcggac gaaatcgaaa aggcggacgc
     4501 cgaagcgagc gggcggctga cgccgaaagt cctgcgcgaa atcgtcaacc tgattcccga
     4561 ccagtggctc ggcgaggcgg aaaaagagcg cgccggttac cttgagtttt tgactgctcg
     4621 cctggaagcg ccgcgcgaat ttgtaaagga ggcggttgat gcacgaagaa atctgcacgt
     4681 ttgattacgc cgttttccgc ctcgtgccgc gcgtcgagcg cgaagaattt tttaacgtcg
     4741 gcgtcatcgt ttcctgcccg gcgcaaaaat ttctcgaagc gcgtaccggg ctcgacgagg
     4801 aaaaattaaa ggctttcgcg cccgcgctcg acgcgaaaac cattcaaagg tatttggaaa
     4861 tcattccgaa aatctgcgcc ggagaggcag ccgcgggcgt catcggacgc cttacgccgc
     4921 gcgagcgttt ttactggctg accgcccagc gcagcacgat tatccaggct tcgcccgtgc
     4981 ataccggatt ctgcgccgac gcgggcgacg tgctccggcg actgttcgac aaaatggtcg
     5041 gctaaggaca tttttccccg aatttgctat catctacaat ttcttatggc aaccacgcag
     5101 acatcttacc cgcgcagtaa aatcaaaatt ctgctgctcg aaaatatttc ggaatcggcg
     5161 gtcgaggaat ttaaagagag cggttacgcc gaaatcagaa aaatttccgg cgctttgagc
     5221 gaggcggatt tggttcgcga aatcaagggc gttcacattc tcggcattcg ctcgaagacg
     5281 cggattacgg aaaagattat cgaaaaggct gaccgtttgc tggcaatcgg cgctttttgc
     5341 atcgggatta atcaggttaa tttgaaagcg gcgaccgcga aaggcgtcgc ggtttttaac
     5401 gcgccttatt caaatacgcg gtcggttgcc gagctggtta tcggtttgtg cgtgatgctg
     5461 attcgccgaa ttacggacaa aaatatggcg gctcatcgcg gcgtctggca gaaagacgcg
     5521 gcgggcagct ttgaattgcg cggcaagact ctgggaatta tcggttacgg caatatcggc
     5581 tcgcaggttt cggtgatggc ggaggctttg ggaatgaacg ttatttatta cgacgttttg
     5641 accaagctgc cgcacggaaa cgccaagcag gaacgcgatt tgcgcgcgct tctgaaaaag
     5701 tcgaacatcg tgacgctgca cgtgccgtcg gacgcgacga cgcgcaatat ga
//
LOCUS       DASWNE010000863         5159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_380249, whole genome shotgun sequence.
ACCESSION   DASWNE010000863 DASWNE010000000
VERSION     DASWNE010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380249"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="VGB68_16840"
     CDS             <1..924
                     /locus_tag="VGB68_16840"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0050963.1"
                     /translation="ELARRNAELVEAHERTNRVFSALTETLPGKVLDDKYRLDLKIGT
                     GGFGVVYRATHLTLERAVAVKVFRPTPGNDSPDALERFRREGVAASKFVHPNAVEVYD
                     FGISANGIAYIVMELLRGFTLSETLSSKGVLPLERSVEIIIPVCAALAEAHAAGVVHR
                     DVKPSNIFLHQTKHGEVVKLVDFGIAKQTRRASEDGFDAPDTGYVLGTSDYMAPERFL
                     SGACDGQVDVYSVGVMFYRMLVGQMPFKSDSPKARLLDDPIPVGGIDASIPAEIQILL
                     ANMLAREPSARPTAAQLTEKLRHLSNAFETV"
     gene            complement(1216..1677)
                     /locus_tag="VGB68_16845"
     CDS             complement(1216..1677)
                     /locus_tag="VGB68_16845"
                     /inference="COORDINATES: protein motif:HMM:NF024297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0050964.1"
                     /translation="MKNLALILFIVLTFCASNALAQTTTTSPAADQTAAIKQTALDYA
                     EGWYEGNAERMERALHPDLAKRIVRNDDKGNSRLENMGALTLVQVTRRGFGKQTPKEQ
                     QQKDVTILDMMENSAVVKLEMRDWVDYMQIGRLNGRWVIVNVLWEMKPPKK"
     gene            complement(1805..2878)
                     /locus_tag="VGB68_16850"
     CDS             complement(1805..2878)
                     /locus_tag="VGB68_16850"
                     /inference="COORDINATES: protein motif:HMM:NF019932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0050965.1"
                     /translation="MNLSKIFPILLLTLLTCCFASAQNAEQQVRKLERAWLDAYEQHD
                     AKAMDAIVADDFTITFTNGSMQTKPQIMNMIKAPRSAGNSSKIYTEEVQARVYGDTVI
                     LIGRVITEWQQNGQTMKEQNRYTDTYVRRGGRWQVVASHLSNVPKPKTRTAEVTSNTG
                     KGRSVNKNKIISQENPIVRIEVDEQLQNVGILNFILKNVARVERYVYASRDENKRIRR
                     LFIAQFEAYLPGVKGGYTFQVINATRLGNFDYQTDIGVYNFAERIAANPGAEPEVTKA
                     LFDRSGLKADEDYLVARYARITDAVDKRHELILFYFESLSDSGFTRAQLESNGKPTPE
                     AEKLYREFAERAARSFKVTDGNS"
     gene            complement(3080..3862)
                     /locus_tag="VGB68_16855"
     CDS             complement(3080..3862)
                     /locus_tag="VGB68_16855"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0050966.1"
                     /translation="MRNIRVLIVDDEPLARRGIRRLLERETDFEIVGEAGNGQAAAAE
                     IESEKPDLVFLDIQMPLAGGFSLIEKAGAKNLPEIVFVTAYDEHAIKAFEAGALDYVL
                     KPIDPERFQKTLGRVRRRINAREAPAVETELIDLLRGLAKSETQNAPHAFLERIPVKE
                     AGRVFFIDAREIDWIGACGNYIEIHTGREKHLLRETMDGIEGKLNPSDFLRIRRSNIV
                     RIEKIKELQPLFNGEFAVILQDGTELASSRRYRKNLETILKF"
     gene            complement(3865..5013)
                     /locus_tag="VGB68_16860"
     CDS             complement(3865..5013)
                     /locus_tag="VGB68_16860"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY0050967.1"
                     /translation="MSPDDQNNSGGTSFFRGATAKGFVIFAGWTLLAFFFSIQAYLNY
                     AYVGRPSPFGRILVAWLSCVYIWAPLTPLVIALAQRFPVERDTIWRRLLVHLFAASLI
                     STLQIAIYTFVRQALLGDATQPFSPVRSFQNLFVAEFHINLLLYGAVLGLTQAYDYYR
                     RYRERERRATQLEIAAAQLETRLAQAQLDALKMQIQPHFLFNTLNTISVLMQDDVDAA
                     NKMLIRLSELLRVALKSEKTQEISLRQELEFLRGYLEIEQTRFQDRLRVDFEVDAETL
                     DSLVPNLILQPLVENAIKHGIAPRAEAGTIRVEARRQNGRVELSVRDDGAGLTESKNH
                     SMGGIGLSNTRARLEKLYGAQHSFEIVSPAAGGLEVKVSIPFRSEAQN"
BASE COUNT         1127 a         1406 c         1392 g         1234 t
ORIGIN      
        1 gagctggcgc ggcgaaacgc cgaactcgtg gaagctcacg agcggacgaa tcgcgttttt
       61 tcggctctga ccgagacttt gcccggaaag gttttggacg acaaataccg actggatttg
      121 aaaatcggca cgggcggctt cggcgtcgtc tatcgcgcca cgcatctgac tctggaaaga
      181 gccgtcgccg tcaaggtttt tcgcccgacg cccggcaacg atagccccga cgcgctcgaa
      241 agatttcggc gcgaaggcgt cgccgccagc aaattcgtgc atccgaacgc ggtcgaggtt
      301 tacgatttcg gcatttctgc aaacggcatc gcgtatatcg taatggaact tttgcgcggc
      361 tttaccttga gcgaaacgtt gagcagtaaa ggcgttttgc cgctcgaacg ctcggtcgag
      421 ataatcattc cggtctgcgc cgcgctcgcc gaagcgcacg cggcgggcgt cgttcaccgc
      481 gacgtaaagc cgtcgaacat tttcctgcac cagacaaaac acggcgaggt cgtcaagctg
      541 gtcgatttcg ggattgccaa acaaacgcgg cgcgccagcg aagacggctt tgacgcgccg
      601 gatacgggtt acgttctcgg cacttccgat tatatggcgc ccgaacgctt tctgagcggc
      661 gcgtgcgacg gtcaggtcga tgtttacagc gtcggcgtga tgttttatcg aatgctggtc
      721 gggcagatgc ctttcaaatc cgattcgccg aaagcgcgtc tgctcgacga cccgataccg
      781 gtcggcggca tcgatgcttc aattccggct gaaattcaga ttctgctggc aaatatgctc
      841 gccagagaac cgtccgcgcg cccgacggcg gcgcagttaa cggagaagct caggcatctt
      901 tcaaacgcct tcgaaactgt gtaacccgtt acagcggcag ccgcgccgaa cgcacgggca
      961 aatgcaaggt ttacactgac gaccgcggag tttgcactga gaaccgcgcg gattacaccg
     1021 gcaactgcgg catttacact ggcgaccgcg gcaattacaa cgacgaccgc agagattaca
     1081 ctcgccaccg cggcaattac aatgacgacc gcagagatta cactgacgac cgcagcaatt
     1141 acacactcgc gaccgcgcgt tacatactag cgaccgcaga aaacacgccg gcgactgcga
     1201 aatgtgcttt tgtgtctatt ttttcggcgg cttcatttcc catagaacgt tgacgatgac
     1261 ccagcgaccg ttgagcctgc cgatttgcat ataatcgacc cagtcgcgca tttccaattt
     1321 tacgaccgcc gaattttcca tcatatcgag aatcgtcacg tccttttgct gctgctcttt
     1381 cggcgtctgc ttgccgaagc cgcgccgcgt cacctgcacc agagtcagcg cgcccatatt
     1441 ttcgaggcgg ctgttgcctt tgtcgtcgtt gcggacgatg cgcttggcta aatccggatg
     1501 cagcgcgcgc tccattcgct cggcgttgcc ttcgtaccag ccttcggcgt aatcgagcgc
     1561 ggtttgttta atcgccgccg tctggtcggc agccggcgaa gtcgtcgtcg tttgcgccag
     1621 ggcgttcgac gcgcagaaag ttaaaacgat aaaaagtatt aaagccaggt ttttcatttt
     1681 gtgttccttt tagtatttat tgattttcta aagcctcagc gcgagacttt ttcatagacg
     1741 aaagatatgt cctgaaacag atacgtgatt tcgccgattt tgttttcggg aaattccata
     1801 aagcttacga gttcccgtcg gtgactttaa agctgcgcgc ggctcgctcg gcaaattcgc
     1861 ggtaaagctt ttcggcttcc ggcgtcggtt tgccgtttga ttcgagctgg gcgcgggtaa
     1921 agcccgaatc gctcaggctt tcaaaataaa acaaaatcaa ttcgtggcgt ttatcgaccg
     1981 cgtcggtgat tcgcgcgtag cgggcgacga gataatcttc gtccgccttc aagccgctgc
     2041 ggtcgaataa agctttcgtt acttccggct ccgcgccggg attggcggcg attctttcgg
     2101 cgaaattata aactccgatg tcggtctggt aatcgaaatt tccgagccgc gtcgcgttta
     2161 taacctgaaa cgtgtagccg cctttaacgc cgggcagata tgcctcaaac tgcgcgataa
     2221 aaagtcgccg gattcttttg ttctcgtcgc ggctggcgta aacgtagcgc tcgacccgcg
     2281 cgacgttttt caaaataaaa ttcaaaatcc cgacgttttg cagctgctcg tcaacctcga
     2341 tgcgaacaat cggattttcc tgcgagatga ttttattttt gtttaccgag cgccctttgc
     2401 ccgtgtttga cgtgacttcc gccgtccggg ttttcggctt cggaacgttc gataaatgcg
     2461 aggcgacgac ctgccagcgc ccgccgcgcc tgacgtaagt gtcggtatag cggttttgct
     2521 ctttcatcgt ctgaccgttt tgctgccatt cggtaatgac gcgcccgatt aaaataaccg
     2581 tgtcgccgta aacccgcgcc tgcacttctt cggtgtaaat cttcgacgaa ttgccggcgc
     2641 tgcgcggcgc ttttatcata ttcatgattt gcggcttggt ttgcatcgag ccgttcgtga
     2701 aagtgattgt aaaatcgtcg gcgacgatag cgtccatcgc tttcgcatcg tgctgctcgt
     2761 aagcgtcgag ccacgcgcgc tccagtttgc gcacttgctg ttcggcattt tgagcggacg
     2821 cgaagcaaca ggtcaggagc gtcaaaagca aaatcggaaa aatttttgat aaattcatta
     2881 cggtttctcg cgtttgaaaa aggtttgtat cgaacaggcg acagcttatt gttatttgcc
     2941 gcgtcgcgca acggttttgt gatatgcggc tggaaatgag tgagaaaagg cggaaacgcg
     3001 agtgtttatg gaacggaaag tggagaacgg aaaataaaat cactgtttaa attttccgtt
     3061 ctcgcgtttc cgcctttgtt tagaatttta aaatcgtttc caaatttttg cgatagcggc
     3121 ggcttgaggc aagctcggtt ccgtcctgca ggatgacggc gaactcgccg ttaaaaagcg
     3181 gctgcaattc cttgattttt tcgatgcgaa cgatgttcga gcggcggatg cgcagaaaat
     3241 ccgacgggtt cagcttgcct tcgatgccgt ccatcgtttc gcgcaaaagg tgtttttcgc
     3301 gaccggtatg aatctcgatg taattgccgc acgcgccgat ccagtcgatt tcgcgggcgt
     3361 ctataaaaaa aacgcgaccg gcttctttga ccggaatgcg ttcgagaaaa gcgtgcggcg
     3421 cgttttgcgt ttcgcttttt gccagtccgc gcagcaggtc gattaattcc gtctcgacgg
     3481 cgggagcctc ccgcgcgttg atgcggcggc ggacgcgtcc gagcgttttt tgaaagcgct
     3541 ctgggtcaat cggtttcaaa acgtaatcga gcgcgcccgc ttcgaaagct tttatcgcgt
     3601 gctcgtcgta agcggtgacg aaaactattt ccggcagatt tttcgcgccc gctttttcga
     3661 tgagcgaaaa tccgcccgcc agcggcattt gaatgtcgag aaaaacgagg tcgggttttt
     3721 cgctctcgat ttcggctgcg gcagcctgcc cgtttccggc ttcgcccacg atttcaaaat
     3781 ccgtttcgcg ctccagcagg cgacgaatgc cgcgccgcgc cagcggctcg tcgtcaacga
     3841 ttaatacacg aatattacgc atttttaatt ttgcgcctca gagcgaaagg gaatcgacac
     3901 cttgacttcc aaaccgcccg ccgccggcga aacgatttcg aagctgtgct gcgcgccgta
     3961 gagtttttcg agtcgcgccc gcgtgttgga aagcccgatg ccgcccatcg aatgattttt
     4021 cgattcggtt aatcccgcgc cgtcgtcgcg taccgaaagc tcgacgcgac cgttttgccg
     4081 gcgcgcctcg acgcgaatcg ttcccgcttc ggcgcgcggc gcgatgccgt gtttgatggc
     4141 gttttcgacc agcggctgga gaatcaggtt cggcacgagc gaatccagag tttcggcgtc
     4201 aacctcgaaa tcgacccgca agcggtcttg aaaccgcgtc tgctcgattt ccagatagcc
     4261 gcgcagaaat tccagttcct gccgcagcga aatttcctgc gttttttcgc ttttcagcgc
     4321 gacgcgcagc aattcgctca gacgaatcag cattttgttg gcggcgtcga cgtcgtcctg
     4381 catcagcacg gaaatcgtgt tgagcgtatt gaacagaaaa tgcggctgaa tctgcatttt
     4441 caaagcgtcg agctgcgcct gcgccagtcg ggtctcaagc tgcgcggcgg caatctcgag
     4501 ctgtgtcgcc cgccgctcgc gctcgcggta gcgccggtaa taatcgtaag cctgcgtcag
     4561 cccgagaacc gccccgtaaa gcagaagatt gatgtgaaac tcggcgacaa acaaattttg
     4621 aaacgaacgg acgggcgaaa acggctgcgt cgcgtcgccc aaaagcgcct gccggacaaa
     4681 cgtgtaaatc gctatctgca aagtcgaaat caagctcgcg gcaaacagat gaacgagcaa
     4741 tctgcgccaa atcgtatcac gctcgaccgg aaaacgctgc gccagagcga tgacgagcgg
     4801 cgtcagcggc gcccagatgt aaacgcagct cagccaggcg accagtattc tgccgaatgg
     4861 cgacggtctg ccgacatacg cgtaattcag ataagcctga atcgagaaaa agaacgccag
     4921 cagcgtccag ccggcaaaga taacaaatcc ttttgccgtc gcgccgcgaa aaaaagacgt
     4981 tccgcccgaa ttgttttggt cgtccggcga catacagacc tattattact cttaaaagcg
     5041 ataaaaaagt caaatcattt tgaactgata actgagaact gataactgat aactgaaatt
     5101 gttttttatc tctcagttct ctgttattaa aaatcttgac ttgtgggacg taagtattt
//
LOCUS       DASWNE010000864         3986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_522209, whole genome shotgun sequence.
ACCESSION   DASWNE010000864 DASWNE010000000
VERSION     DASWNE010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522209"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="VGB68_16865"
     CDS             <1..855
                     /locus_tag="VGB68_16865"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEY0050968.1"
                     /translation="EAYSGASNPLRVGIEKAAANIFAAVDTACDNAGLNQGDIIAIEC
                     GLAGVRRADLRQILRRLVSHRFRVKTVEVITDAEIALFAATQGKEGLCVIAGTGSVCI
                     GQSSNGEKAIAGGWGPLAGDEGGGAGIARRALQAVAKASDGRGQSTKLSDLAVDYFRA
                     GRLEDLSVAIYAPPIDNARIAGFARSVIETAQAGDPIACAVLDEAGRELGLAAKAVIK
                     QLKLARRKFPIGYVGGIFHAGELICQPMLETVHEIAPKAFISKPEFSPAVAAAQMALA
                     TFRNGGKR"
     gene            994..1890
                     /gene="murQ"
                     /locus_tag="VGB68_16870"
     CDS             994..1890
                     /gene="murQ"
                     /locus_tag="VGB68_16870"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF003915.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEY0050969.1"
                     /translation="MLPITEQENPNTSRIDRVSTLEAIRLINAEDKKVALAVEKALPE
                     IALAIDKIVERMQNGGRLFYVGTGTSGRLGVLDASEIPPTFGVSYDLVQGVIAGGYDA
                     LYKATESSEDDGGAGTEDLKKRGLNERDAVVGIAASGRTPYTIGALEYARRLGCFTAC
                     VTCVPDSAITKAAEIALIAEVGAEAIAGSTRMKAGTAQKLILNMISTAAMIRLGYVKG
                     NRMTNVKSSNAKLKERSVRILMTETDLDEAGAQNLLNEANGDLRVAIVMQKTNVSRVI
                     AEKALTDNNFVIEKAIGVCAGK"
     gene            1895..2521
                     /locus_tag="VGB68_16875"
     CDS             1895..2521
                     /locus_tag="VGB68_16875"
                     /inference="COORDINATES: protein motif:HMM:NF040120.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HEY0050970.1"
                     /translation="MNKKSIIGFIISVISVLGINFIPLEMLLYKDYSGETTMVVYALE
                     NVLTIFLATVFILLFAPRRDQTGEIKTRKELVQVYLISTLSLTVGSGVLLSIFIFWIL
                     RADVTFSAVATAMIWISGFLVLEFIADFMMLRPLSLAQADNFLTRSVRRVFLLFLCVF
                     LGIFLAAFVSSGFVAPFIIMKTLADIADQIEIYQGFRGKTVGNKLNRA"
     gene            2647..>3986
                     /locus_tag="VGB68_16880"
     CDS             2647..>3986
                     /locus_tag="VGB68_16880"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEY0050971.1"
                     /translation="MQTLDLVIIFGYLIGVTVFGVWFSSRQETTEDYFVGDRNVPWWA
                     IAASIVATETSTITFISVPGIAFAKNGNFQFLQLVFGYMLGRVVISLIFIPLYFKGEL
                     QTVYQLLGERFGTKVKMLASALFVVMRNIADGIRLLLTAIVLGYVYLAFHLKSYPDAN
                     QNEILATAIIVSIILLGIVMIIFTFYGGMEAVIWVEVVQLAIYIVGAIAAAFVIANQV
                     DGGFSGAIAAGEQFQKFSLIDWGFRQTSYNLPIPFLSDGWQFSLPFDFTKTYTFWAGL
                     VGGCFLTMSTHGTDQYLVQRYLCTDKPRRAALALLSSGAVVLGQFIGFLFIGVLLFAF
                     YQPFNAPEYAAATGAFPFTGGDKVFPDFITKFMPSGLSGLVVAAIFAAALSSSLNSIA
                     ATAVNDLYKPFAADKSDRHLLRIAGWLTVAIGIVQIGVALVVMKQERSALDSALS"
BASE COUNT          866 a          981 c         1095 g         1044 t
ORIGIN      
        1 gaagcatact cgggggcgtc aaatcctctg cgcgtcggaa tcgaaaaagc ggcggcgaac
       61 attttcgcgg cggtcgatac cgcctgcgac aatgccggat taaatcaggg cgatattatc
      121 gccatcgaat gcgggctggc aggcgtgcgc cgcgccgatt tacggcaaat tctgcgccgc
      181 ctggtctcgc accgttttcg cgtcaaaacg gtcgaagtca tcaccgacgc cgaaatcgcg
      241 cttttcgcgg cgacgcaggg caaagaaggt ttgtgcgtga ttgccggaac cggctcggtc
      301 tgcatcggtc aaagctcgaa tggcgaaaaa gccatcgccg gcggctgggg accgctcgcc
      361 ggcgacgaag gcggcggcgc gggaattgcc cggcgcgcgc tgcaagccgt cgccaaagct
      421 tcggacgggc gcgggcaatc aacaaagctg agcgatttgg cggtcgatta ttttcgcgcc
      481 gggcggcttg aagatttgtc ggttgccatt tacgcgccgc cgattgataa tgcgagaatc
      541 gccggatttg cgcgctcggt catcgaaacg gcgcaggctg gcgacccgat agcctgcgcc
      601 gttttggatg aagccggtcg cgaactcgga ttggcggcaa aagccgtgat aaagcaatta
      661 aaactggcgc gccgaaaatt tccgatcggc tacgtcggcg gaatttttca cgcgggcgaa
      721 ctgatttgcc agccgatgct ggaaacggtt cacgaaatcg cgccgaaagc atttatttcc
      781 aagccggaat tttcgcccgc cgtcgccgcc gcgcagatgg ctctggcgac gtttagaaac
      841 ggaggaaagc gatgaagttt tttttcgcgg gcttttaata atttcccttt gtcgcttttc
      901 ggcggcaaat caaacagcaa attcgtaacc gttgccaaag ttgtcgtaaa tctatttacg
      961 ttttattaaa tatctattga cgttctataa aaaatgctgc cgattaccga acaggaaaat
     1021 ccgaacacta gccgcatcga ccgcgtctcg acgctcgagg cgattcgtct aatcaacgcc
     1081 gaagacaaaa aagtcgccct cgcggtggaa aaagctttgc ctgaaattgc gcttgcgatt
     1141 gacaaaatcg tggagcgaat gcaaaacggc gggcggcttt tttatgtcgg aacgggaacg
     1201 agcgggcggc tcggcgtttt ggacgcgagc gagattccgc cgaccttcgg cgtttcgtat
     1261 gatttggtgc agggcgttat cgccggcggc tacgacgcgc tttacaaagc gacggaatcg
     1321 agcgaggacg acggcggcgc gggcacagaa gatttgaaaa aacgtgggct gaacgagaga
     1381 gatgcggtcg tcggaatcgc cgcttcgggc agaacgcctt acaccatcgg agcgctcgaa
     1441 tacgcgcggc ggctcggttg ttttacggct tgcgtgacct gcgttcccga ctccgcgata
     1501 acgaaagcgg cggaaattgc gctaatcgcc gaagtcggcg cggaagcgat tgccggctcg
     1561 acccgaatga aagccggaac ggcgcagaaa ttgattttga atatgatttc gacggcggcg
     1621 atgattcgac tcggctacgt caagggaaac cgtatgacga acgtcaaatc ctcgaacgcc
     1681 aagctcaaag agcgctctgt aagaatttta atgacggaaa ccgatttgga cgaagccggg
     1741 gcgcagaatt tattaaacga ggcaaacggc gatttgcgcg tcgcgattgt gatgcaaaaa
     1801 acgaacgttt cgcgtgtaat tgcggaaaaa gctctgacgg ataataattt tgtgattgaa
     1861 aaagcaatcg gcgtttgcgc cggaaaataa aattttgaat aaaaaatcta tcatcggctt
     1921 tatcatctcg gttatttcgg ttctcggaat caatttcatt ccgcttgaaa tgctgcttta
     1981 taaagattat tcgggcgaaa cgacgatggt agtttacgcg ctcgaaaacg tgctgacgat
     2041 atttctggcg accgttttca tcctgctttt cgcgccgcgc cgcgaccaaa cgggagagat
     2101 taaaacgcgc aaagagcttg tgcaagtgta tcttatatcg acgcttagcc tgacggtcgg
     2161 aagcggagtt cttctttcga tttttatttt ctggattttg cgcgcggacg ttactttttc
     2221 ggcagtcgcg acggcgatga tttggatttc cggctttctg gtgctggaat ttatcgccga
     2281 ttttatgatg cttcgaccgc tttcgctggc gcaggcggac aactttctga ctcgcagtgt
     2341 ccgcagagtt ttcctgcttt ttctctgcgt ttttctcggc atatttctcg ccgcttttgt
     2401 cagcagcggg ttcgtcgcgc cgttcatcat tatgaaaacg cttgccgata tcgccgacca
     2461 gattgagatt tatcagggat ttcgcggtaa aacggtcggc aacaaattga atcgagcgta
     2521 aaaggctttc tattttcgag gctaaattgc aaaataaagc gtaaccggtt gaccaatcgt
     2581 cgttttccgg ataaaaattt tttatagctt ttagaaatca ttttcttttt tcttatcgct
     2641 ggcacaatgc aaactttaga tttggtcatc attttcggtt atttaatcgg cgtcacagtg
     2701 ttcggcgtct ggttttcgag ccggcaggaa acgacggaag attattttgt cggcgaccgg
     2761 aacgtgccct ggtgggcgat tgccgcttcg attgtggcga ccgaaacctc gacgattacg
     2821 tttatctccg tgccgggaat cgcttttgcg aaaaacggga atttccagtt tttgcagctc
     2881 gttttcggtt atatgctcgg tcgcgtcgtg atttcgctga tttttattcc gctttatttc
     2941 aaaggcgaat tgcagaccgt ttaccagctt ttgggcgagc gcttcgggac gaaagtcaaa
     3001 atgctcgctt cggcgctgtt cgtcgtaatg cgtaatatcg ccgacggaat ccgcctgctt
     3061 ttaaccgcga ttgtactcgg ttacgtttat ctggcttttc atttgaaatc ttacccggac
     3121 gccaatcaaa atgagattct cgccacggca attatcgttt ccataattct gctgggaatc
     3181 gtgatgatta ttttcacttt ttacggcgga atggaagcgg tgatttgggt cgaagtcgtg
     3241 cagctcgcca tctacatcgt cggcgcgatt gccgcggctt ttgtcatcgc caatcaggtc
     3301 gacggaggat tttcgggcgc gattgccgcg ggcgagcaat ttcaaaaatt cagtttgatt
     3361 gattggggct ttcgccagac gagctacaat cttccgattc cgttcttatc cgacggctgg
     3421 cagttttcgc ttccctttga ttttacgaaa acatacacgt tttgggctgg actcgtcggc
     3481 ggctgttttt taacgatgtc gacgcacgga accgaccaat atctcgtcca gcgctatcta
     3541 tgcaccgaca agccgcgtcg tgccgcgctc gctctgcttt cgtcgggcgc ggtcgtttta
     3601 ggacagttta tcggttttct tttcatcggt gttttgcttt tcgcgtttta tcaaccgttt
     3661 aacgcgcccg aatacgcggc ggcgacgggc gcgtttcctt ttaccggcgg cgacaaggtt
     3721 tttcccgatt ttattacgaa atttatgcct tccgggcttt ccggtttagt ggtggcggcg
     3781 attttcgcgg cggctctctc ctcgtctctt aattcgattg ccgccacagc cgttaacgat
     3841 ttatataaac cttttgccgc ggataaatcc gacaggcatc tgctgcgaat cgccggttgg
     3901 ctgacggtcg caatcggcat cgtccaaatc ggcgtcgctc tggtagtgat gaaacaggaa
     3961 cgctcggcgc tggattcggc gctttc
//
LOCUS       DASWNE010000865         3094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_99477, whole genome shotgun sequence.
ACCESSION   DASWNE010000865 DASWNE010000000
VERSION     DASWNE010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99477"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="VGB68_16885"
     CDS             <1..965
                     /locus_tag="VGB68_16885"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEY0050972.1"
                     /translation="LGGAMTHNSISGVAHFRTKNEAECIEKIRQLVSELPKKEETKVS
                     ITEPAKPKNKPEKLYEILPADHRAPYDMHEVLRCFLDDGAIDEFQADYAREMICGTAR
                     IGGILVGVIANSRGMVKGKSGKPPKFGGIVYTESAEKTAYFIENCNRHGTPLLFVQDV
                     SGFMVGADAEHSGIIRAGARFVEAMACAVVPKIVLTINHASGAGYYAMAGQGFDPDFI
                     FSLPTGRMGVMEGDSAVQAIFGTQLEKLKKEGGEPDEALKAEMEKVRETYERELDSKH
                     AAARGLVDAIVTPERLHSALQIALQTCLNTDKPHIGAFVLPPLE"
     gene            1033..2610
                     /locus_tag="VGB68_16890"
     CDS             1033..2610
                     /locus_tag="VGB68_16890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050973.1"
                     /translation="MPPRVEGTNQNQEVYKPKPVEPKIEKPKTVEVNQLSNTDKNLFL
                     RQNSLGADTLKISLFARFEQANFRQNPTAPPVNQADPQKVEDAVNLIKDRLSEGFLGD
                     VSHGDLSDIQNSFRNLNSAEANEVFRGLSDGDLQKWADELDGTLGGFSGDEKKALFNE
                     LAGKLDDQQLARFINALGDEGNIQSLADSVANNASGATKAALIGRMTGNVETNERAAI
                     AVAELIGGMGGNPRELEGVLQNMSQTQLAAVMKAASQEEFHSNAMPFGGVSTYTTFEP
                     QPLVDMLSAVSRTGNAELKANVFQAASMELKRIEEAGGLLNQVPGRADAARDVRNALT
                     GLLQSDTTGIMTELEHNFRNGDGISAYTKSMLNAGETTELGNIVARLSKGNDLRGDPL
                     ARFGTQERGTDGNPHYRNAQVLGYFAGALFSGAKQITSDRSRQADMLKNVFGTIAGAT
                     GAANPASGVVSSILNGLTQQIVSDVTNGLNKGTMDMEEALEQLIFPRSAQTGDTYEGA
                     AEADYDSAFSRVVLRNS"
     gene            2764..>3094
                     /locus_tag="VGB68_16895"
     CDS             2764..>3094
                     /locus_tag="VGB68_16895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050974.1"
                     /translation="MKIEEIPLPPEARAGAIEGRYEIELDGATNTILSELKKNYDKTE
                     EKILFLSNEAEPSKIFEFYQPKLAEKGFSRDASVQASGRNYQQTIWRNESWLGGQAVA
                     VAVIDAGK"
BASE COUNT          804 a          775 c          886 g          629 t
ORIGIN      
        1 cgctcggcgg cgcgatgacg cataactcga tttccggtgt agcgcatttt cgcacgaaaa
       61 acgaagcgga atgcatcgag aaaatccgtc agctcgtgtc tgaattgccg aaaaaagagg
      121 aaacgaaggt gtccatcacc gaaccggcaa aaccgaaaaa taaacctgaa aaactttacg
      181 agattctgcc cgcagatcat cgcgcgccgt atgatatgca cgaagttctg cgctgctttt
      241 tggacgacgg agcgattgac gaatttcaag ccgattacgc gcgcgagatg atttgcggca
      301 cggcgcgcat cggcggcatt ctcgtcggcg tcattgccaa ctcgcgcgga atggtcaagg
      361 gaaaatcggg aaaaccgccg aaattcggcg gcatcgttta cacggaatcg gctgaaaaaa
      421 cggcttattt tatcgaaaac tgcaatcgtc acgggacgcc tctcctgttc gtgcaggacg
      481 tttccggctt tatggtcggc gcggatgccg aacattcggg aatcatccgc gccggcgcga
      541 gatttgtcga ggcgatggct tgcgccgtcg tgccgaaaat cgttttgacg attaatcacg
      601 cttcgggcgc gggttattac gcgatggctg gacagggttt cgacccggat tttattttca
      661 gtttgccgac gggcagaatg ggcgtgatgg aaggcgattc ggcggtgcag gcgattttcg
      721 gaacgcagct cgaaaaattg aaaaaagaag gcggggaacc ggacgaagca ttgaaagccg
      781 agatggaaaa agtgcgcgaa acctacgagc gcgagctgga ttcgaaacac gccgccgcgc
      841 gcggtctggt cgatgcgatt gtcacgcccg aacgcttgca cagcgctttg cagattgcct
      901 tgcaaacctg tctgaatacg gacaaaccgc acatcggcgc gttcgttttg ccgccgctcg
      961 aataaaaaat caggaaactc aggcgagatt gtgccgataa tgtaggcaag acatttttat
     1021 caggagaaaa atatgccgcc gagagtcgag ggaacaaatc agaatcagga agtttacaaa
     1081 ccgaaaccgg ttgagccgaa aatcgaaaag ccgaaaacgg ttgaggtcaa tcagctttca
     1141 aacacggata aaaatctgtt tctcagacaa aacagcctgg gcgccgacac gctgaaaatc
     1201 agcttattcg ccagatttga acaagctaat ttccgccaaa atccgaccgc gccgcccgtt
     1261 aatcaagccg acccgcaaaa ggtcgaagac gccgttaatt taatcaaaga ccgcctgagc
     1321 gagggttttc tgggcgacgt ctcgcacggc gatttgtccg acattcaaaa ctctttcaga
     1381 aatttgaaca gcgccgaagc caacgaagtt tttcgaggtc tttccgacgg cgatttgcag
     1441 aaatgggcgg acgaattgga cggcacgctc ggcggcttca gcggcgacga aaagaaagct
     1501 ctttttaacg agttagccgg aaaactggac gaccagcagc tggcgcgttt tataaatgcg
     1561 ctcggcgacg agggaaatat tcagagcctc gcggattcgg tcgccaacaa tgcttcgggc
     1621 gcgaccaaag ccgcgcttat cggcagaatg accggcaatg tcgaaaccaa cgaaagagcg
     1681 gcgatcgccg tcgccgaatt gattggcgga atgggcggca atccgcgcga gctggaaggc
     1741 gttctgcaaa atatgagcca gacgcagctg gcggcggtga tgaaagccgc ttcgcaggaa
     1801 gaatttcatt cgaacgccat gcccttcggc ggcgtatcga cttacaccac attcgagccg
     1861 caaccgctcg tcgatatgct cagcgccgtt tccagaaccg gcaacgcgga gctgaaagcg
     1921 aacgtttttc aggcggcgtc gatggaattg aaacgtatcg aagaagcggg cggactgttg
     1981 aatcaggttc ccggacgggc tgacgcggcg cgcgacgtgc gcaacgctct gaccgggctt
     2041 ctgcaaagcg acacgaccgg aattatgacc gagctcgaac ataatttccg caatggcgac
     2101 ggcatctcgg cttacaccaa gtcgatgctg aacgcgggcg agacgaccga actcggaaac
     2161 atcgtcgcgc ggctttccaa aggaaacgat ttgaggggcg acccgctggc gagattcggc
     2221 acgcaggagc gcggaaccga cggcaacccg cattacagaa acgcacaggt gctcggctat
     2281 ttcgcgggcg cgctgttttc gggcgcaaaa caaatcacat ccgaccgcag ccgccaggcg
     2341 gatatgctga aaaacgtttt cggcacgatt gcgggcgcga ccggcgcggc gaatccggct
     2401 tccggcgtcg tctcttcgat tctcaacggc ttgacgcagc agattgtgtc ggacgtgacg
     2461 aacggcttga acaaaggcac gatggatatg gaagaagcgc tggagcaatt gattttcccg
     2521 cgcagcgcgc aaaccggcga cacttacgaa ggcgcggcgg aagccgatta cgattccgcc
     2581 ttcagccgcg tggttttgcg aaattcgtaa gcgatttcga tagcccacaa attaaagcgg
     2641 agagaatttt ctttccgctt tttttcgttt gatgtgttat attttttgcc gatgaagaag
     2701 ttttaccgaa ttttaccgat tatttttctt gcgttattat tttcgtgcaa tcaaaaggct
     2761 tttatgaaaa ttgaagaaat tccgctgccg cccgaagcgc gggctggcgc aatcgaaggc
     2821 agatatgaaa tcgagcttga cggcgcgacc aatacgattt tgagcgagct gaaaaagaat
     2881 tacgacaaaa ctgaggaaaa aattctgttt ttgtcgaatg aagccgagcc gtcgaagatt
     2941 tttgagtttt atcaaccgaa gctggcggaa aaagggtttt cccgcgacgc gagcgttcag
     3001 gcaagcggtc ggaattatca gcaaacaatc tggcgcaatg aaagctggct tggcgggcaa
     3061 gccgtcgcgg ttgccgtaat cgacgcggga aaag
//
LOCUS       DASWNE010000866         5121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_411489, whole genome shotgun sequence.
ACCESSION   DASWNE010000866 DASWNE010000000
VERSION     DASWNE010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411489"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="VGB68_16900"
     CDS             complement(<1..595)
                     /locus_tag="VGB68_16900"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY0050975.1"
                     /translation="MNQTEKVRDFFDVVSEEYRGKYEKRQVFHNYFFNQRLEQATGGL
                     DFGGKTVLDIGAGTGNLYDYIVAKNDTVDFYACDISAKMLEQSNIPADNRFVGKCYEI
                     DFPIRKFDYIFMLGVTTYLSSEEMEKTAEFIYGKLADDGLAIITFTNRQGLDTITRTL
                     SKNVIKLFKLKNKVISQSFRIYTYSLNQVKSLYQGKLR"
     gene            complement(744..2627)
                     /gene="asnB"
                     /locus_tag="VGB68_16905"
     CDS             complement(744..2627)
                     /gene="asnB"
                     /locus_tag="VGB68_16905"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEY0050976.1"
                     /translation="MCGIVGIVNQISRPVEREILEKMNRAIRHRGPDEDGFYLNENVG
                     LAMRRLSIIDLAGGQQPIHNPSKTAWIVFNGEIYNFQELRAELEKRGHRFYTNSDTEI
                     ILHLYDEHGADCVTHLRGMFAFAIWDEREKSLFLARDRVGKKPVLYSHQPNGDLIFGS
                     EFQALLQHPDVDREVDYEAIHQYLTFSCVPAPLTAFRQIRKLEPGHWLRWKNGEIKTE
                     RYWTPDFSAKIKISEQDAVEETLRILREATKMRLISEVPLGAFLSGGVDSSTVVALMA
                     EASEKPVKTFSIGFEEQDFSELKYARRVAEHIGAEHHEFIVKPDAIEVLPTLVEHYGE
                     PFADSSAIPTFYVSKETRKHVTVALNGDGGDETFAGYERYFAIQLAEKYKRIPSILRK
                     SVIEPAAAFLPTSMGQHSRVKSAKNFLAVSSMPKSSLYLRWMSAINEDLKAELYSENF
                     KQKTSNDSTASVLQSWFARANGSGAIDAMLLTDTMTYLPNDLLVKVDIASMANSLEAR
                     SPFLDHKVIEFAASLPEKIKVRGTETKYLLKKAASRVVPPEVLYRRKMGFGIPVGYWL
                     RNEMKDFMFEILLSEKSFNRGLFKPEIIKKLVAQHVAEKQNHTYPLWTLLMLELWFRQ
                     MID"
     gene            complement(2652..3998)
                     /locus_tag="VGB68_16910"
     CDS             complement(2652..3998)
                     /locus_tag="VGB68_16910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050977.1"
                     /translation="MEKVLLVLCVVFGLIISVFLFPDGMGATLLCAVTASIAVMIINR
                     HEDEKEFLVQVFLLGLFFRMVLGATIYTFGWGEFLGGDSRTYDQLGYTLLQVWYGEVP
                     ETNEYAKRATMMSGSGWGMYYLVAGIYSIFGRNPLAAQMFSSVIGAATAPAAYICSYG
                     IFHNKRVAKASALIVALFPSLILWSCQLLKDGFIIFLLILAMALVMRLQEKFDYVSTA
                     LLILSLFAILTLRFYIFYMVAIAVVGSFVIGLSNSAQAIVRRAIVIMFIGLGLTYLGV
                     LRNAGTEIEQYGNLERIQQSRQNLAEAGSGFGEDLDVSTSAGAIAAIPVGFLYLMLAP
                     FPWQIGNFRQAITLPEQILWWCSIPLLLGGLWYTIKYRLRNAIAILIFTIMLTLAYSI
                     FQGNVGTAYRQRAQIQVFLFIFVAAGWTLMKERRENTKRLHDFERDRVRQRLQVKT"
     gene            complement(4003..>5121)
                     /locus_tag="VGB68_16915"
     CDS             complement(4003..>5121)
                     /locus_tag="VGB68_16915"
                     /inference="COORDINATES: protein motif:HMM:NF028171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEY0050978.1"
                     /translation="KVMGLAAYGKPEYYDFFSKKVLTTNGTGDFHYNIKILDHHLAKH
                     YQFPEGIVKELGEPRKKGEELTEHHWNIASSAQKVVEDTAIYLAHRIKEMTGEENLCM
                     AGGVAFNSVMNGRIFHETPFKNFYVQPAAGDAGCSLGAALMVWHQKLGNPRKFVMNHS
                     YWGPKFSNEECRKALDEANLKYETLSDEVLLPRLAKMISEGAIIGWFNGRMEWGPRAL
                     GARSFLADPRRADMREVLNHKVKLREWFRPLAPSMLEEYGREVFGVEHHDPFMITVIQ
                     VAEEYKKKIPAVVHVDGTARPQMVSREVNPRYWNLINEFKKLTGIPMLLNTSFNIQEP
                     IVCTPQDAINTFRNANFDALVLENNLVLNSSSSSSSGK"
BASE COUNT         1264 a         1356 c         1249 g         1252 t
ORIGIN      
        1 ttcgcagctt gccctgataa agactcttga cttgattcag tgaataggtg taaatccgaa
       61 aagactgcga aatcacctta ttttttaatt taaacagctt gataacattt ttgctgagcg
      121 tccgcgttat tgtatctaat ccctgccgat tagtgaaggt gatgattgcc aaaccgtcgt
      181 ccgccagctt gccgtaaata aattccgccg ttttttccat ctcctcgctg ctcagataag
      241 tcgtcacgcc gagcataaaa atataatcga atttccgaat ggggaaatct atctcgtaac
      301 attttccgac aaacctgttg tcggcgggaa tgttgctctg ctccagcatt ttagcggaaa
      361 tatcgcacgc gtaaaaatcg accgtatcat ttttggcgac gatatagtca tacaaattgc
      421 cggttcccgc gccgatgtcg agaacggttt tgccgccgaa atccagcccg ccggtcgcct
      481 gttcgagtct ttgattgaaa aaataattgt gaaaaacctg acgcttttca tactttccac
      541 gatattcttc ggaaacgacg tcaaagaaat cgcgcacttt ttcggtttga ttcataattt
      601 tataaaacaa tgcaaagatg ccgcaaagaa atgaaaccac agatttgcac ggataaacac
      661 gagatattga acaggacgga aaggataata tctttctttt atccgcgtcc gtccgtgtaa
      721 atctgtggtt aaaaattctt ccgtcagtca atcatttgcc ggaaccagag ttccagcatc
      781 aacagcgtcc agagcggata cgtgtgattt tgcttttccg cgacgtgctg cgcaaccagc
      841 tttttaataa tttccggctt gaaaagcccg cgattgaagg atttttccga cagcagaatc
      901 tcaaacataa aatccttcat ctcgtttctg agccagtagc cgaccggaat tccgaaaccc
      961 atcttccggc gatacaaaac ttccggcggc acgacgcggc tcgccgcttt ttttaaaaga
     1021 tacttggtct ccgtgccgcg cacttttatt ttttcgggca aactggcggc aaattcgatg
     1081 accttgtggt cgagaaacgg cgagcgggct tcgagcgagt tcgccatcga agcgatatcg
     1141 accttgacca gcaaatcgtt cggcagataa gtcatcgtgt ccgtaagcag catcgcgtca
     1201 atcgcgcccg agccgtttgc tctggcaaac cagctttgca gaacgcttgc cgtcgaatcg
     1261 tttgacgttt tttgtttgaa attttcgctg taaagctcgg ctttcaaatc ttcgttgatt
     1321 gccgacatcc agcgcaggta aaggctcgat ttcggcatcg aagaaaccgc cagaaaattt
     1381 ttcgccgatt tcacgcggct gtgctgtccc atcgacgtcg gcagaaacgc ggctgccggc
     1441 tcaatcacgg attttcgcag aatcgacgga attcttttat atttttccgc cagctgaatg
     1501 gcaaaataac gctcgtaacc ggcgaaagtc tcgtcgccgc cgtcgccgtt taaagcgacc
     1561 gtgacgtgtt ttctggtttc cttcgaaacg taaaaggtag gaatcgccga agaatcggcg
     1621 aaaggctcgc cgtaatgctc gaccagagtc ggcaaaacct cgatggcgtc gggcttcacg
     1681 ataaattcgt gatgctccgc gccgatgtgc tcggcgacgc ggcgcgcgta tttcagctcg
     1741 ctgaaatcct gttcttcaaa gccgatggaa aaggttttga cgggcttttc gctcgcttcc
     1801 gccatcagcg cgacgaccgt cgaagaatcg acgccgccgg aaagaaacgc gccgagcgga
     1861 acttcgctga ttaagcgcat ttttgtagct tcgcgcaaaa tcctcaaagt ttcttcgacc
     1921 gcatcctgct cggaaatttt tattttggcg gaaaaatccg gcgtccagta tctctcggtt
     1981 tttatttcgc cgtttttcca tctgagccag tgtccgggtt cgagcttgcg gatttggcga
     2041 aaagccgtca gcggcgcggg cacgcaggaa aacgttaaat attgatgaat cgcctcgtaa
     2101 tcgacttcgc ggtcgacgtc cggatgctgt aataatgcct gaaattccga gccgaaaatc
     2161 aagtcgccgt tcggctgatg cgaataaaga accggttttt tgccgaccct gtcgcgcgcc
     2221 agaaacagcg atttttcgcg ctcgtcccaa atggcaaagg cgaacattcc gcgcaggtgc
     2281 gtgacgcagt cggcgccgtg ttcgtcgtaa agatgcagaa tgatttccgt gtcggaattg
     2341 gtgtaaaacc ggtgtccgcg tttttccagc tcggcgcgca gttcctgaaa gttgtaaatc
     2401 tcgccgttaa aaacaatcca agccgttttt gaagggttgt gaatcggctg ctgtccgccc
     2461 gccaaatcga taatcgaaag cctgcgcatc gccagcccga cgttttcgtt cagatagaaa
     2521 ccgtcttcgt cgggtccgcg atggcggatt gcccgattca ttttttcgag gatttcgcgc
     2581 tcaaccgggc gggaaatttg attgactata ccgacaattc cgcacattta taaatcactt
     2641 gacgaaaaat cttacgtttt aacctgcagt ctctgcctca cgcggtcgcg ctcaaaatcg
     2701 tgcagacgct tggtgttttc gcgtctttcc ttcatcagag tccagcccgc cgcgacaaaa
     2761 ataaaaagaa aaacctgaat ctgcgcgcgc tgccggtacg ccgtgccgac gttgccctga
     2821 aaaatcgaat aagccagcgt cagcataata gtaaaaatca aaatggcaat cgcgtttcgc
     2881 agccgatact tgatcgtata ccacaaaccg cccagcagaa gcggtatcga acaccaccag
     2941 agaatctgtt cgggcagcgt aatcgcctgg cggaaattgc cgatttgcca cggaaacggc
     3001 gcgagcatca gataaagaaa accgaccgga atcgccgcga tggcgcccgc gctggtcgag
     3061 acgtcgaggt cttcgccgaa tcccgaaccg gcttccgcca gattctgccg gctctgctgg
     3121 atgcgctcga gattgccgta ttgctctatc tcggtgcccg cgtttcgcaa aaccccgaga
     3181 taagtcagtc ccaaaccgat aaacattatg acgattgcgc gccggacgat tgcctgcgcg
     3241 gaattgctca gaccaatcac gaagctgccg acgacggcaa tcgccaccat ataaaagata
     3301 tagaaacgaa gcgtcaggat ggcgaaaagc gaaagaatca gcaaagccgt cgaaacgtaa
     3361 tcgaattttt cctggagccg catcaccagc gccattgcca gaatcaaaag aaagatgata
     3421 aatccgtctt tcagcaattg gcacgaccag agaatcagcg agggaaacaa ggcgacaatc
     3481 agggcggaag ctttggcgac gcgtttgtta tgaaaaatcc cgtaagagca gatataagcg
     3541 gctggcgcgg tggctgcgcc gatgaccgag ctgaacatct gcgcggctaa aggatttcgc
     3601 ccgaagatgg aatagatacc ggcgaccaga taatacattc cccaaccgga accggacatc
     3661 atcgtggcgc gcttggcata ttcgttggtt tcgggaactt ctccatacca gacttgcagc
     3721 agcgtataac cgagctggtc gtatgtcctc gagtcgccgc cgagaaattc gccccagccg
     3781 aaggtgtaaa tagtcgctcc cagaaccatc cggaaaaata agccgagcag aaatacctgg
     3841 acgagaaatt ctttttcgtc ctcgtgacga ttgataatca taaccgcgat actggcggtg
     3901 acggcgcaga gcagtgtcgc gcccattccg tcgggaaaca gaaagacgct gattatcagc
     3961 ccgaatacta cacaaaggac gaggaggact ttttccattt agctacttcc cactactact
     4021 actactacta ctgtttaaaa ccagattatt ttccagaacg agggcgtcga aattggcgtt
     4081 tctgaaagta ttgatggcgt cctgcggcgt gcagacaatc ggttcctgaa tattgaacga
     4141 agtgttcaga agcatcggaa tgccggtcag cttcttaaac tcgttgatta aattccagta
     4201 gcgcggattg acctcgcggc tgaccatctg cgggcgcgcc gtgccgtcga cgtggacgac
     4261 cgccggaatt ttctttttgt attcttcggc gacctgaatg acggtaatca taaacgggtc
     4321 gtgatgctcg acgccgaaaa cctcgcgccc gtattcttcg agcatcgacg gcgcgagcgg
     4381 gcgaaaccat tcgcgcagtt ttactttatg attcagaacc tcgcgcatat cggcgcggcg
     4441 cgggtctgcc agaaagcttc tcgcgcccag cgcgcgcggt ccccattcca tccgaccgtt
     4501 aaaccagccg ataatcgcgc cttcggaaat cattttcgcc agtcttggaa gaagcacttc
     4561 gtcggagagc gtttcgtatt tcagattggc ttcgtccaga gctttccggc attcctcgtt
     4621 ggaaaatttc ggtccccaat acgaatgatt catcacgaat tttcgcgggt ttcccaattt
     4681 ttgatgccag accatcaagg ctgcgcccaa agaacagccc gcatcgcccg ccgccggctg
     4741 gacatagaaa tttttaaacg gcgtttcgtg aaaaatccgc ccgttcatca ccgaattgaa
     4801 agccacgccg cccgccatac acaagttttc ctcgcccgtc atttccttga tgcggtgcgc
     4861 cagataaatc gccgtatcct cgacgacttt ttgcgcgctc gaagcgatgt tccagtggtg
     4921 ctcggtcaat tcctcgcctt ttttgcgcgg ctcgcctagt tctttgacga tgccttcggg
     4981 aaattggtaa tgcttcgcca ggtgatggtc taaaattttt atgttgtaat gaaaatcgcc
     5041 cgtgccgttg gtcgtcaaaa cctttttcga gaaaaaatcg taatactcgg gctttccgta
     5101 agccgccaag cccatcactt t
//
LOCUS       DASWNE010000867         2093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_380349, whole genome shotgun sequence.
ACCESSION   DASWNE010000867 DASWNE010000000
VERSION     DASWNE010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380349"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="VGB68_16920"
     CDS             <1..1333
                     /locus_tag="VGB68_16920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050979.1"
                     /translation="ENNSPVAVFWREKAVQAAYFNVSVPLDAELTQPYFLKSPRQNYH
                     YVQQDFTNQNFPFQYPLLAGEATLEINGETIPFVQSVEYRYADDIRGEVRRDLNVVPK
                     VSLNLDQKLLIVPLGRNRKRQITMSVTNNSSGAVKGRAKLNLPERWQAQPEQADFDLK
                     IKGAKTSIVFNVTIPRMSAVANYKIGAEAEADGEKFTREMHTLAYPHIETHRYYTAAE
                     TAVNVFDLKSAPVRVGYVMGSGDAVPEAIRQMGLSVELLSENDLNGGDLSRFDVIVVG
                     IRAFQVRQDLISNNQRILEYARSGGNLIVQYQRPEYENLLPFPAKIGARVADENAKVT
                     ILEANNPVFNFPNKITDKDFENWVQERNLYSFSTFDGGYKPLLESHDANEAENKGGLV
                     VAEIGKGKYVYTSYAFFRQLPAGVSGAYRIFANLLSLPKSAAPRRNTKAES"
     gene            complement(1330..>2093)
                     /locus_tag="VGB68_16925"
     CDS             complement(1330..>2093)
                     /locus_tag="VGB68_16925"
                     /inference="COORDINATES: protein motif:HMM:NF016354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HEY0050980.1"
                     /translation="RYNTDERLTRVTPEFCRRTIREIEKLQKELRKTLGVTFAFLGDE
                     IYLKAGLPIPTRRHYGNYPQIEDGVGMIRSFLNSFESVLKKAEKRESAKAETNYRAAE
                     AQSFTENVRTHKLALVPKTKDRIQTTEARNQNSKSLTGTILTGEMFAPILRRKIEELN
                     SLIGSKLDVLAVKNNYFGGDVAVAGLLTGQDYLAAKDRIRGDFVIIPQHTIKSDEPIL
                     LDGMNFDDLKAQFPVPVYPLDLKGLIQFIQNRSGF"
BASE COUNT          507 a          509 c          544 g          533 t
ORIGIN      
        1 tgaaaacaat tctccggttg ccgttttttg gcgcgaaaaa gccgttcaag ccgcttattt
       61 caatgttagc gtcccgcttg acgcggaatt gacgcagcct tattttctta aatctccgcg
      121 tcaaaattac cattacgtcc agcaggattt tacgaatcaa aattttcctt ttcaatatcc
      181 gctgctcgcc ggagaggcga cattggaaat taacggcgaa acgattcctt tcgttcaatc
      241 cgtcgaatat cgttatgccg acgacattcg cggcgaagtc cggcgcgatt taaacgtcgt
      301 gccgaaagtc tccttaaatc tcgaccagaa gcttttaatc gttccgctcg gcagaaaccg
      361 gaagcggcag attacgatga gcgtgacgaa taattcgtcg ggcgcggtca aaggacgggc
      421 gaagctgaat ctgcccgagc gctggcaggc gcagcctgag caggcggatt ttgatttgaa
      481 aataaaaggc gctaaaacgt caatcgtttt taacgtgacg attccccgga tgtccgccgt
      541 ggcgaattat aaaatcggcg cggaagccga ggcggacggc gaaaaattta cgcgcgaaat
      601 gcacacgctg gcttacccgc atatcgaaac gcaccgttat tacaccgccg ccgaaaccgc
      661 ggttaacgtc ttcgatttaa aatccgcgcc cgtcagggtc ggttacgtta tgggcagcgg
      721 cgacgccgtt ccggaagcga tccggcagat gggtttgagc gtcgagcttt taagcgaaaa
      781 cgatttaaac ggcggtgatt tatcgcgttt cgacgtgatc gtcgtcggaa ttcgagcctt
      841 tcaggtgcgg caggatttga tttcaaataa tcagagaatt ctcgaatacg cgcgcagcgg
      901 cggaaattta atcgtccagt atcaacgccc cgaatacgaa aatctattgc cttttccggc
      961 gaaaatcggc gcgcgcgtcg ccgacgaaaa cgcgaaagtg acgattctgg aagcgaataa
     1021 tccggttttt aattttccga ataaaattac ggacaaagat tttgaaaact gggtgcagga
     1081 acgaaatctt tacagctttt cgacttttga cggcggttat aaaccgctgc tcgaatcgca
     1141 cgacgccaat gaagcggaaa ataaaggcgg cttggtcgtg gcggaaatcg gaaagggaaa
     1201 atacgtctac acttcttacg ctttttttcg ccagcttccg gcgggcgtct ccggcgcata
     1261 tcgaattttt gcgaatcttt tgagcttgcc gaaaagcgcc gctccgcgta gaaacacgaa
     1321 agcggaaagc taaaatccgc tccggttttg gataaattgg attaaacctt ttaaatccaa
     1381 aggataaacc ggcacgggga attgcgcttt gaggtcgtcg aaattcattc cgtccagcag
     1441 aatcggctcg tcggatttga ttgtgtgctg cggaataatg acgaaatcgc ctctgattcg
     1501 gtctttcgcg gcgagataat cctgtcccgt taaaagtccc gcaacggcga cgtcgccgcc
     1561 gaaataatta tttttaaccg cgagaacatc gagctttgag ccgatcagcg aatttaactc
     1621 ttcgattttc cgccgcagaa tcggcgcgaa catttcgccc gtcagaattg tgccggtcag
     1681 agattttgaa ttttgatttc gggcttcggt cgtctgaatt cggtctttgg ttttgggaac
     1741 aagcgctaat ttgtgagttc tgacgttttc ggtaaaactc tgcgcctctg cggctctgta
     1801 gttagtttcc gcctttgccg actcgcgctt ttccgccttt ttcagcacgc tctcgaatga
     1861 attcagaaac gagcgaatca tcccgacgcc gtcttcaatc tgcgggtaat tgccgtaatg
     1921 acggcgcgtg gggataggca gaccggcttt cagataaatt tcgtcgccga gaaaagcgaa
     1981 ggtgacgccg agcgttttgc gcaattcttt ttgcagcttt tcgatttcgc ggatggtgcg
     2041 gcggcaaaat tcgggcgtca cgcgcgttaa tctttcgtcc gtgttgtagc gcg
//
LOCUS       DASWNE010000868        10162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_488262, whole genome shotgun sequence.
ACCESSION   DASWNE010000868 DASWNE010000000
VERSION     DASWNE010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10162
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488262"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..683)
                     /locus_tag="VGB68_16930"
     CDS             complement(72..683)
                     /locus_tag="VGB68_16930"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HEY0050981.1"
                     /translation="MNDVKFNKILIFINSLVPLALLISDAVGGRLGANPIEFFLRTTG
                     VLTLVFLLITLAVTPLRKMSGWNNLIKFRRMLGLYAFFYGFLHLITYGIFDRNFNFSA
                     IAGDVWQRPFIAVGMLAFFLLIPLALTSTGGMIKRLGGKNWARLHKLSYLIAILGVIH
                     FWMIVKSDLTYPFFFAFILAILLGYRIYSSLKSKAANPKLAAK"
     gene            complement(741..1700)
                     /gene="msrP"
                     /locus_tag="VGB68_16935"
     CDS             complement(741..1700)
                     /gene="msrP"
                     /locus_tag="VGB68_16935"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003767.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HEY0050982.1"
                     /translation="MVIKKPGEIKSSQITDEKTYLNRRNFLRAGLLAGTPPATAGLYR
                     FFNQPPPKEIKTEIIESVAKSSLSVDDKLNSFEEITNYNNFYEFSTGKTAVARASENF
                     VTRPWTVEVGGMVQNPKTFDIEEILKFEQEERIYRFRCVEGWSMVIPWVGFPLKKILD
                     AVEPLGGAKYVAFQTLYDSAQMQSSFSAGIDFPYVEGLRLDEALHPLTILATGLYGKL
                     MPNQNGAPLRLVVPWKYGFKSIKSIVKITLTDIEPPTAWNIAARSEYGFYSNVNPQVA
                     HPRWSQSTERRIGEYAQRETLLFNGYAEQVASLYQGMDLKKFF"
     gene            2059..3936
                     /locus_tag="VGB68_16940"
     CDS             2059..3936
                     /locus_tag="VGB68_16940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050983.1"
                     /translation="MLISRFISLLVIFSIACGSVFAQLPTKKSDPEKEKAASELEKNA
                     VELLEQAVGETSALKLPENRALIYAMAGDLLWSKDEKRARSLFRGAAGEIVQIINTKA
                     ENSDSRFPETGNGVTPAFNPRQAEIFGLRQMVLRTLAEHDAEMALEILQTTRPTEVAA
                     EMQTYSMPAPAPNPAQKQPTPAPVARNFRVEQEIRLEQGLLAKAAEQDPSKAAQRIRE
                     NLEKGFSTEILSALQKIYKKDAELATKLFDETIQKLLGSDLSKNQNMIFAFNLLRPYT
                     FPPKENPNVKSPPQLSIDEKSAKDIANKIADTFMKATNAREIAFLNSVMSILQKIVPE
                     RVAQLKQKEAALRKQTPQNTRAIQAPASLNDPNATPEKMIADATRAQPQMRGSLYRQA
                     AFRGVSGGDAEKIRALLQSQPQSKERDDALAYLDANLVLNQLRAGKTDEARRIIDRLP
                     FGAAKAELLVQLAVALYRLNTKESKENALRIMSEAREMVKDFPEDKDEMDGLVKVIAG
                     FAVIDPERAFTMLSPVVEQVNEVINAQAVLARFNKQTQSFRDGELVITNSFGALNSKL
                     FRYGRELKMLAESDFTRTRGLIDQMRRDDVRVFVKLFVAQSILKEKIGLAGGISFGGG
                     Q"
     gene            4097..5959
                     /locus_tag="VGB68_16945"
     CDS             4097..5959
                     /locus_tag="VGB68_16945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050984.1"
                     /translation="MKPFRFLCLPLILVFVFNSIFAQTAPNPAGDAEKEKARREQEKQ
                     QEVEKKAVAMLGEIAAAAETLKLAVNRVSVFSSLAVLLWKYDEKQARNLVNIAVEEII
                     VAQNADDDEQSDASQLTWQIMESRRQLVQSLAAHDADFALRIFRRTRAPEIAALMQSP
                     KNVLPNGNDYNRLQNEINTEQMLINEIVRQDPKRAIKLAREILEKGVSYALLDLIERI
                     REKEPEEAARLADEIVRKFQEMDFSARHNERHLAVSFISRLSQKNESGGAEKSKFVVD
                     ENGLRALAEKIAVFFLRDPQVENYYYQISGFITIIEKFSPSRANQLRGREIEIKQKQA
                     QTNPYERMNQINQDSDADTLLKEAQNAPSELKNQYYTYAANKLIGSDDTERARAVINS
                     ISDRRAREYALQNLNSNLFYKALGAGNVAEARRLAAQSINKSQAIAFYIQIFNYARQK
                     EDDKLARRILDEAASLVTSNPENSVEMGALFQLANAYSSVEPETSLTLIESSIGKFNE
                     LLNASILLNRFAGNETNREDEITSQFFHSTLSQYGFYFNQSDFARLATVSFERAKGLG
                     DGFQRPEARIFTRLMIAHSILSQIRSNNNGVYNLTKERNRELAVLPVYRIQTTP"
     gene            complement(5935..6081)
                     /locus_tag="VGB68_16950"
     CDS             complement(5935..6081)
                     /locus_tag="VGB68_16950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050985.1"
                     /translation="MQARQKYGRSAAAKIARQASGAQSNIVGELLIESFQGIESFQGV
                     VCIL"
     gene            6194..7894
                     /locus_tag="VGB68_16955"
     CDS             6194..7894
                     /locus_tag="VGB68_16955"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0050986.1"
                     /translation="MLAINEILQGRYRILRRLGHGGMGAVYEAMDERFGSPVALKEII
                     IDLDRVHNASQQAMMRQAFEREARILAHLHHEAFPHVRDYFSETDRQFLVMELIGGDD
                     LADLLARRQEPFLLEDVLKWADQLLDALDYLHTLEMPVIHRDLKPQNLKLTARGKIKL
                     LDFGIAKGTDAQIANTITNKTFIAATLNYSPLEQILPVLDPSVREFIIYSHGEKVDDI
                     LQQPTDARSDLYALGATLYHLSTGAVPTDVLKRTLEIWAGKPDPLPNPATVNPNIPKA
                     VSDVLLKAMEVERGNRYNSAKEMREALSQAAATRRTVEEEARYLLWLAHQDQLKAEQD
                     RLNNEAPALQTQQQTPENQAAGITDPVAAAVPTQNSSEFTRQSYLAAILPEPPKPEPA
                     KEETSVRTLPAADKNVAAAPPKNRKNLWLLPVAALFLLTLGGAIFGMWWIMGKTVNET
                     AKPTPTSTGTPESVVEATPELPTSTFDSSVVVPAQSPSITPTSQTPTSQTPTSTPRLP
                     IASTPAPAVSTPTPRLSTPPPKTPPPARTPPPTPRPQSGCPQAPPRGCRQLPSCVIEC
                     "
     gene            8143..9861
                     /locus_tag="VGB68_16960"
     CDS             8143..9861
                     /locus_tag="VGB68_16960"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0050987.1"
                     /translation="MPAINEVLQQGRYCIIQPLGQNGMGAVYEAYDNVRETKVLFKEI
                     SVNLRKVTTLNQQETLKRRFADEAKILTGIKHESLLPVHDYFSEIDRHYLVMEMTDGN
                     NLSELLERGKKPFALSEITNWAEQLLDALHYLHTQTPPVIHRDIKPQNVKLTADGKIK
                     LALGIQNPDAKMHATVTNQTLHYLPLEQIWVGLDSASQKVIVNSYDEKSERILEQPAD
                     AKSDIYALGATLYHLLTARLPVDALERSIEILEGKPDPLTLPARLNPDIPPEISDILM
                     KALEIKRENRFDSAVIMRQAWRTALVRVRERAGPEAKKQDEDVLEIPFAGHKQPKPER
                     RLAAPESLKTEAENAQQIELIKRQLQEAEAQRLKAEQRAAEAEKQLAEKQSEVPVNTE
                     SAATTIKSTEAFIKTSPAPTAISPDVLAYGATPEQSPDEFKDLFVVPPKENNFFRRMF
                     AVAAALVILCGAFWGIWILLKSSPIESNRTTSSQTMSLTDTGKPASSVEAAPSPSVET
                     TPETSAIETPALAPDAAETTAASPTLKNKPLSTAPVRAKKQTSPSTKPAAEKKAVTVD
                     DIINDN"
     gene            9972..>10162
                     /locus_tag="VGB68_16965"
     CDS             9972..>10162
                     /locus_tag="VGB68_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050988.1"
                     /translation="MKFASLPRVKLTYPYLFSFFLLTLSMLSLTFVPQSARAQQMPLS
                     LADILIGLRSKKVILYERN"
BASE COUNT         2635 a         2752 c         2575 g         2200 t
ORIGIN      
        1 taagctgttt cgtgcttttt ctgtttcgtg ctttttcttt ttcgcctcaa tttttttcgc
       61 gttctttcct gttatttcgc ggctaatttc ggattcgccg ctttcgattt caggcttgaa
      121 taaatcctgt agccgagcag aatcgccaga ataaaggcga agaaaaacgg gtaggtcaaa
      181 tccgatttca caatcatcca aaagtgaatc acgcctaaaa tcgcaatcag ataactcaat
      241 ttatgcaggc gcgcccagtt ttttccgccg agccgtttta tcattccgcc ggtcgaggtc
      301 aaggcaagcg gaatcagcag aaaaaacgcc agcatcccga cggcgataaa cgggcgctgc
      361 cagacatctc cggcaatcgc cgaaaagtta aaattcctgt caaaaatgcc gtatgtaatc
      421 aaatgcagaa agccgtaaaa gaaagcgtaa agtccgagca tccggcgaaa ttttatcaga
      481 ttattccagc ccgacatttt tctgagcggc gtcaccgcta aagttatcaa caaaaaaacc
      541 agagtcaaaa cgccggtcgt ccgcaaaaaa aactcgattg gatttgcgcc caatctcccg
      601 ccgacggcgt cgctgatgag caaagcgagc ggcacgaggc tgttgataaa gattagaatt
      661 ttgttgaatt ttacatcgtt cataaagtaa ttagccgtca gtaatcagcc gtcagcaggc
      721 tgaaaactga ctgctgacgg ctaaaaaaac tttttcaaat ccattccttg ataaaggctc
      781 gccacctgtt cggcgtagcc gttaaaaaga agcgtttcgc gctgcgcgta ttcgccgatg
      841 cgtctttccg tcgactgcga ccagcgcggg tgcgcgacct gcgggttgac gttggaataa
      901 aaaccgtatt cgctgcgcgc cgcgatgttc caggcggtcg gcggctcgat gtcggtcaga
      961 gtgattttga cgattgattt gatgctcttg aagccgtatt tccagggcac gaccagccgc
     1021 agcggcgcgc cgttctgatt cggcatcagt tttccgtaaa gtcccgtcgc cagaatcgtc
     1081 aaaggatgca aagcttcgtc cagccgcaag ccttcgacat acgggaaatc gattccggcg
     1141 ctgaatgaag actgcatctg cgcggaatcg taaagcgtct gaaaagcgac gtattttgcg
     1201 ccgccgagcg gctcgaccgc atccagaatc ttctttaaag gaaaaccgac ccagggaatg
     1261 accatcgacc agccttcgac gcagcgaaag cgatagattc tttcctcctg ctcgaatttt
     1321 aaaatttctt cgatgtcgaa agttttcgga ttttgcacca ttccgccgac ctcgaccgtc
     1381 cacgggcgcg tcacaaaatt ttcagacgcg cgcgcgacgg cggttttgcc ggtcgaaaac
     1441 tcgtaaaaat tgttgtaatt ggtgatttcc tcgaaagaat tgagtttatc gtcgacgctc
     1501 agactggatt tcgcgacgct ttcgattatc tcggttttaa tttccttcgg cggcggctga
     1561 ttgaaaaagc ggtaaagccc ggcggtcgcc ggcggcgttc ccgccagcag tccggcgcgc
     1621 agaaaattgc ggcggttcag ataagttttc tcgtccgtta tctggctgga ttttatctcg
     1681 cccggctttt tgataaccat aattctttat tcgctccggc ttgcgggttg gtttcaatct
     1741 acaaaatatt cctgatttat attatcttat gctaagattt tattccctga aacttcttcc
     1801 ggcaaaaagg agataaagat gcggcgagaa gctcgctttg ccggcgacgg cggatttccg
     1861 gcgggcgaaa gttttgattg aacaatttcg ccgcgaagaa ttgcgcctgc cggtcaaagc
     1921 ctctgacgag gcagagctat tttcaaagaa aaaccggctc tgcggaaacg aattcttttt
     1981 attacgaggg attttaggcg aatcattcgc gcagaattta tttttacgct tttctctgga
     2041 aagaaaaagg agattttaat gttgatttcg cgatttattt cactgctggt tattttttca
     2101 atcgcctgcg gctcggtttt cgctcagctt ccgacgaaaa agtcagaccc ggaaaaagaa
     2161 aaagccgcgt ccgagcttga gaaaaacgcg gtcgaattac tggaacaagc cgtcggcgag
     2221 acctccgcgc tgaagcttcc ggaaaaccgc gcgttgattt acgcgatggc gggcgacctg
     2281 ctctggtcaa aagacgaaaa acgcgcgcgc agtctttttc gcggcgcggc aggcgaaatc
     2341 gttcaaatta ttaatacgaa agcggaaaat tccgattcgc gttttccgga aacgggaaac
     2401 ggcgtaacac cggcgtttaa cccgcgacag gcggaaattt tcggcttgcg gcagatggtt
     2461 ttgcgaacgc tggcggagca cgacgcggaa atggcgctcg aaattttgca gacgacgcgc
     2521 ccgaccgaag tcgccgccga aatgcaaacc tactcgatgc ccgcgcccgc gccgaacccg
     2581 gctcaaaaac aaccgacgcc cgcgcccgtc gcgcgcaatt ttagagtaga gcaggaaata
     2641 cggctcgaac agggtctgct cgccaaagcc gccgagcagg atccttcaaa agccgcgcag
     2701 agaattcggg aaaatctgga aaaaggcttt tcgacggaaa tcctttcggc tctgcaaaag
     2761 atttataaaa aagacgccga gctggcgacc aaactttttg acgaaacgat acaaaagctg
     2821 ctcggctcgg atttatcaaa aaaccagaat atgattttcg ccttcaacct gctgcgccca
     2881 tacacttttc cgccgaagga aaatccgaac gtgaaaagcc cgccgcagct gtcgattgac
     2941 gaaaaatcgg cgaaagacat cgccaacaaa atcgccgaca cgtttatgaa agcgacgaat
     3001 gcgagagaaa tcgcctttct gaactccgtg atgtcgattt tacagaaaat cgtgccggaa
     3061 agagtggcgc agctgaaaca aaaagaagcc gcgctgagaa aacaaacgcc gcaaaatacg
     3121 cgcgcgattc aagctccggc ttcgctgaac gacccgaacg cgacgcccga aaaaatgatt
     3181 gccgacgcga cccgcgccca gccgcagatg cgcggctcgc tctatcgtca agccgctttt
     3241 cgcggcgttt ccggcggcga tgccgaaaag attcgcgctc ttttgcagag ccagccgcaa
     3301 agcaaagagc gcgacgacgc gctcgcttat ctggacgcca acctggtgct gaatcagctg
     3361 cgcgcgggaa aaacggacga ggcgcgcagg attatcgacc gcctgccgtt cggcgctgcc
     3421 aaagccgagc tgctggttca gttagccgtc gccttatatc gtctgaatac aaaggaaagc
     3481 aaagaaaacg ccttgcggat aatgagcgag gcgcgcgaga tggtcaagga ttttcccgaa
     3541 gacaaggacg aaatggacgg cttggtgaaa gtcatcgccg gtttcgccgt tatcgacccg
     3601 gaacgcgctt tcacgatgct ttcgcccgtc gtcgaacagg tcaacgaagt cattaacgcg
     3661 caggcggtgc tggcgcgctt taacaagcag acgcaaagct tccgcgacgg cgagctggtc
     3721 atcaccaaca gtttcggcgc gcttaattca aagctttttc gctacgggcg agaattgaaa
     3781 atgctggcgg aaagcgattt tacgcgcacg cgcgggctga tcgaccagat gcgccgcgac
     3841 gacgtgcgcg tattcgtcaa gctttttgtc gcgcagagca ttctgaagga aaaaatcgga
     3901 ctcgccggcg gcatatcttt cggcggcggt caataaaccg acgcggcggg tttatcgcat
     3961 actttgcacg cgcgccggat tttctgaatt taaaatcggc acgcgcgccg aaaggattta
     4021 taatttccgg cgcgcgtgca accttttctt tcttcaatcg ttcaaaattt ataattcgcc
     4081 cggagaagga aaatctatga aaccgtttcg ctttctttgc ctgccgctga ttcttgtttt
     4141 tgttttcaat tcgatatttg cccaaaccgc gccgaatcct gccggcgacg ccgaaaagga
     4201 aaaagcgcgc cgcgaacagg aaaagcaaca ggaagtcgag aaaaaagcgg tcgcgatgct
     4261 cggcgaaatc gccgccgcag ccgaaactct gaagctcgcc gtcaaccgcg tctcggtttt
     4321 ctcttcgctc gccgttctgc tttggaaata cgacgaaaaa caggcgcgaa atctggttaa
     4381 tatcgccgtc gaggaaatca tcgtcgctca aaatgccgac gacgacgagc agagcgacgc
     4441 ttcccagctc acctggcaga taatggaatc gcgccgccag ctcgtccagt cgctcgcggc
     4501 gcacgacgcc gattttgcgc tccggatttt tcgccggacg cgcgcgccgg aaatcgccgc
     4561 gctgatgcag tcgcccaaaa acgttctccc gaacggcaac gactataacc gcctgcaaaa
     4621 tgaaatcaac accgagcaga tgctcatcaa cgaaatcgtc cgccaggacc cgaaacgagc
     4681 cattaagctg gcgcgcgaaa ttctcgaaaa aggcgtctcc tacgcgctgc tcgatttaat
     4741 cgagcgcata cgggaaaaag aacccgaaga agcggcgcgg ctggcggatg aaatcgtgcg
     4801 caaatttcag gagatggatt tttcggcgcg ccataacgag cgccacctcg ccgtcagctt
     4861 tatctcgcgt ttatcgcaaa agaacgaatc gggcggcgcg gaaaaatcga agttcgtcgt
     4921 cgacgaaaac ggcttgcgcg cgctggcgga aaaaatagcc gtttttttcc tgcgcgaccc
     4981 gcaggtcgaa aactactatt accagatttc ggggtttatc acgattatcg aaaaattctc
     5041 gccgtcgcgt gcaaatcaat tgcgcgggcg cgaaattgaa atcaaacaaa aacaggcgca
     5101 gaccaacccg tatgaacgga tgaaccagat aaatcaggac tcggacgcgg atacgctgct
     5161 caaggaagcg cagaatgcgc cgtcagagct taaaaaccag tattacactt acgccgccaa
     5221 caagctcatc ggcagcgacg acacggagcg cgcccgcgca gtgataaatt cgatttccga
     5281 ccgccgcgcg cgcgaatacg ctctgcaaaa tctcaacagc aacctgtttt acaaagcgct
     5341 cggcgccgga aacgtcgccg aggcgcgccg gctcgccgcg cagtcgatta acaaaagtca
     5401 ggcgattgct ttttatatcc aaatttttaa ttacgcgcgc cagaaagaag acgacaagct
     5461 cgcccgccgg attttggacg aagccgcttc gctcgtcacg tcaaatcccg aaaacagcgt
     5521 cgagatgggc gcgcttttcc aactggcaaa cgcttattcg agcgtggagc ccgaaacctc
     5581 tctgacgctg attgaatcaa gcatcggaaa attcaacgag cttttaaacg cctcgattct
     5641 gcttaaccgg tttgccggca atgaaacgaa tcgagaggac gaaatcacgt cgcagttttt
     5701 tcacagcacg ctctcgcaat acggttttta ttttaaccag agcgattttg ccaggctggc
     5761 gacggtcagc tttgagcgcg caaagggttt gggcgacggc tttcagcgac cggaagcgcg
     5821 gatttttacg cgtttgatga ttgcgcactc gattttgtcg caaattcgca gcaataataa
     5881 cggcgtttat aatttgacga aagaaagaaa tcgagagctt gccgttttac ccgtctacag
     5941 aatacagacg acgccttgaa agctttcgat tccctgaaag ctttcgatta ataattcgcc
     6001 gacgatattt gattgcgcgc cgctcgcttg ccgcgcaatt ttcgccgccg ccgaacgtcc
     6061 gtatttctgc cgcgcctgca aatttaacgt aaatttcgcg cccgttattt tagctaaacc
     6121 ttgcttttct tttttgagaa tttagttaat aatcggctga agaaactttt cgcgttttaa
     6181 aactacaggc aaaatgctgg cgataaacga aattttacag ggacgctatc gaatcctccg
     6241 acggctcggg cacggcggta tgggcgcggt ttacgaggcg atggacgagc gtttcggctc
     6301 gccggtggcg ctcaaagaaa tcattatcga tttggacaga gttcataatg ccagccagca
     6361 ggcgatgatg cggcaagcct ttgagcgcga agccagaatt ctcgcccacc tgcatcacga
     6421 agcgtttccg cacgtccgcg attatttttc cgaaaccgac cgccagtttc tggtgatgga
     6481 attaatcggc ggcgacgatt tagccgatct gctggcgcgc cgccaggagc cttttttgct
     6541 cgaagacgtc ttgaaatggg ctgaccaatt gctcgacgcg ctcgactatc tgcacacgct
     6601 ggagatgccg gtgattcacc gcgatttgaa gccgcagaat ctgaagctga cggcgcgcgg
     6661 caaaataaaa ttactcgatt tcggcatcgc caaaggcacg gacgcgcaga ttgccaatac
     6721 gattaccaat aaaacgttta tcgccgcgac gctcaactac tcgccgctcg aacagatttt
     6781 gcccgtgctc gacccgtccg tccgcgagtt tataatctac agccacggag aaaaagtcga
     6841 cgacattctg cagcagccga ccgacgcgcg aagcgattta tacgcgctcg gcgcgacgct
     6901 ttatcatctt tcgaccggcg cggttccgac cgatgtttta aaaagaactc tggaaatctg
     6961 ggctggaaag cccgaccctc tgccaaatcc ggcgacggta aatccgaata tcccgaaggc
     7021 agtttcagac gttttgctga aagcgatgga agtcgagcga ggcaatcgct acaactcggc
     7081 aaaggaaatg cgcgaagcgc tgagccaagc cgcagccacg cgccgcacgg tagaagaaga
     7141 agccagatat cttttgtggc tggcgcatca ggaccagctg aaagccgagc aggacagact
     7201 gaataacgag gctccggcgc tccaaaccca acagcagacg ccggaaaacc aagccgccgg
     7261 aatcaccgac ccggtcgccg ccgccgtccc gacgcaaaac tcttcggagt ttacccgcca
     7321 gtcatatctg gcggcaattt tgcccgaacc gccgaaaccc gaaccggcga aagaagaaac
     7381 gagcgtccgg actctgcccg ccgccgacaa aaacgttgct gccgcgccgc cgaaaaatcg
     7441 aaaaaattta tggcttttgc ccgtcgcggc tttgttttta ttgacgctcg gcggcgcgat
     7501 tttcggtatg tggtggatta tgggcaaaac cgtaaacgag acggcgaagc cgacgccgac
     7561 ttcaaccgga actcctgaat cggtagtcga agccactccc gaactcccga catcgacgtt
     7621 cgacagctcg gtcgtggttc ccgcgcaatc gccttcaatt acgccgacct cgcaaacgcc
     7681 gacttcgcaa acgccgacct cgacgcccag gctgccgata gcttcgacgc ccgctccggc
     7741 agtttcgacg ccgacgccga gattatcgac tccgccgccg aaaacgccgc cgccggcgcg
     7801 aactccgccg ccgactccgc gcccgcaatc gggctgtccg caggcgccgc cgcgcggctg
     7861 tcgtcaattg cccagctgcg ttatcgagtg ttaatacgcc ggcgggtaag ctcaatttat
     7921 taaccccttc tctagtcacc ggctttcgcg ccggacgctt atctaacgcc tatcgaaaca
     7981 cgattctctg gcgacaaacg gcttcttacc gtcacgaaat aaacttaaaa acaggatttg
     8041 taaaaatttg tttaaaattt taaaacaata tctttcattt tttgcggaaa tggttataat
     8101 gtatttagca gacacttcac ctgtttgttc aaacccaata atatgcctgc cataaatgaa
     8161 gttttacaac aaggacgcta ctgtataatt caaccgctcg gacaaaacgg gatgggcgcg
     8221 gtgtatgaag cctacgacaa tgttcgcgaa acaaaagtcc tgttcaagga aatttcggtt
     8281 aatttaagaa aagtcacgac cctgaatcaa caggaaacgc tcaagcgccg ctttgccgac
     8341 gaagcgaaga ttctgaccgg catcaaacac gaatctctgc tgcccgtcca cgattatttt
     8401 tcggaaatcg accgtcatta tctggtgatg gagatgaccg acggcaataa tctgagcgag
     8461 ctgctcgaac gcggcaaaaa gccgtttgcg ctttcggaga taaccaattg ggcggagcaa
     8521 ttgctcgacg cgcttcacta tctgcacacg cagacgccgc ccgttattca tcgcgatata
     8581 aaaccgcaaa acgtgaaact gaccgcggac ggcaaaatca agctcgccct cggcattcaa
     8641 aatccggacg ccaagatgca cgcgaccgtg accaatcaaa ccctgcatta tttaccgctc
     8701 gaacagattt gggtcgggct cgattcagcg tcgcagaaag taatcgtcaa cagctacgac
     8761 gaaaaatcgg agcggatttt agagcagccg gctgatgcca aaagcgatat ttacgcgctc
     8821 ggcgcaacgc tttatcatct gctgacggcg cgacttccgg ttgatgcgct ggagcgctcg
     8881 attgaaattc tggaaggcaa acccgaccct ttgacgcttc ccgcgcggct taatccggat
     8941 attccgccgg agatttccga cattttaatg aaagctctgg aaatcaagcg tgaaaaccgt
     9001 tttgattcgg cggtaataat gcgtcaggct tggcggacag ccctggtgcg cgtcagggaa
     9061 cgtgccggac cggaagcgaa aaaacaagac gaagacgtat tggaaattcc ttttgccggg
     9121 cataagcagc cgaagccgga acgcagactc gccgcgccgg aaagcttaaa aacggaagcg
     9181 gaaaacgcgc agcagataga gttaatcaag cgccaattgc aggaagccga agcccaaaga
     9241 ttaaaagccg aacagcgcgc cgccgaagcg gaaaagcagc tggctgaaaa acagtctgaa
     9301 gttccggtca ataccgaatc tgcggcgaca actataaaat cgacggaagc ttttatcaaa
     9361 acatcgcccg cgccgacggc tatttcgccc gacgtactcg catacggcgc aacaccggaa
     9421 caatcgcccg acgaatttaa agatttattt gtcgtcccgc cgaaggaaaa taattttttc
     9481 cgtcgaatgt ttgccgtcgc cgcggctctg gtaatattat gcggcgcgtt ttggggaatt
     9541 tggattttat tgaaatcgtc gccaatcgag tcgaaccgga cgacctcgag ccagacgatg
     9601 tctctgaccg ataccggcaa acccgcgtcg agcgtcgaag ccgcgccgtc gccgagcgtt
     9661 gaaacgacgc ccgaaacgag cgccatagaa acgcccgctt tagcgccgga cgcagccgaa
     9721 acgaccgccg cttcgccgac gctcaaaaat aaacctcttt cgacggctcc ggtgcgagcc
     9781 aaaaaacaaa cctcaccttc gaccaaaccg gcggcggaaa agaaagccgt cacggtcgac
     9841 gatattatca atgataatta aataagccgc ccgaaaacat tataagagtt gctgtttcgg
     9901 agcgggcttc agacttttaa attttgccga aaaatttaag ccaatctatc cattcatcaa
     9961 ggaacaaaag tatgaaattc gcatctcttc cccgagtcaa attaacttat ccgtatcttt
    10021 tctcgttttt tttattaacg ctgtcgatgc tttcgctgac gttcgttccg caaagcgcgc
    10081 gggcgcagca gatgccgctc agcctggcgg atattttaat cggcttgcgg tcaaagaaag
    10141 taattttgta tgaaagaaac ag
//
LOCUS       DASWNE010000869         2169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_312426, whole genome shotgun sequence.
ACCESSION   DASWNE010000869 DASWNE010000000
VERSION     DASWNE010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312426"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..370
                     /locus_tag="VGB68_16970"
     CDS             107..370
                     /locus_tag="VGB68_16970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050989.1"
                     /translation="MEAAENQITINTLAVGTRLVVQCKKDWRMAVVSAISEEKIVLQI
                     CSPSGRTYRKSCTLETIIDSSGAIPFLGEGVWQESLVKYDSRW"
     gene            complement(542..1108)
                     /locus_tag="VGB68_16975"
                     /pseudo
     CDS             complement(542..1108)
                     /locus_tag="VGB68_16975"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
     gene            complement(1169..1336)
                     /locus_tag="VGB68_16980"
     CDS             complement(1169..1336)
                     /locus_tag="VGB68_16980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050990.1"
                     /translation="MKMTLFLLLATCFGLAACNMENAVLTAENRNVGNETSPLAMEKT
                     PVLVELYTSEG"
     gene            1437..>2169
                     /locus_tag="VGB68_16985"
     CDS             1437..>2169
                     /locus_tag="VGB68_16985"
                     /inference="COORDINATES: protein motif:HMM:NF036484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050991.1"
                     /translation="MQASVKTKEVIEVRGARVHNLKNISFSIPVNELTVVTGVSGSGK
                     SSLAFDTVYAEGQRRYVESLSAYARQFLERMDKPDVDEVIGIAPAIAIRQKNSTRNPR
                     STVATQTEIYDYLRLLFARVGQTFCHVCGREVKKDSPESAAEETLKTLAEGARFYVIF
                     PASDADLSYETKSKVTAHLLSLMQKGFTRLFRGGETLELQKPEDYNFSDFDDTFVLID
                     RLKADESIRQRLVDSLEICFRESRTA"
BASE COUNT          529 a          542 c          530 g          568 t
ORIGIN      
        1 caatggagcg cgccgatatg gaaaaagttt taagctggct cgacgcgggc aaaaatccgt
       61 cgctgattca gatgccggtc gaatattata aatcttatca aaactcgtgg aagctgccga
      121 aaatcaaata accattaaca ctctcgccgt cggaacgcgt ctggtcgtgc aatgcaaaaa
      181 agactggcga atggcggtcg tctcggcgat ttccgaagaa aaaatcgttt tacagatttg
      241 ctcgccttcg gggagaacct atcgaaaaag ctgcacgctc gaaactatca tcgattcgag
      301 cggcgcgatt ccctttttag gcgaaggcgt ctggcaggaa agcctcgtca aatacgattc
      361 acgctggtga aggattttta ccatcaggat acaaagaaca caaagaagaa tttacagtga
      421 taaaggggat ggacagggat aaaacaaaag ccttatgatt ttgctttatc accttaatcc
      481 cctttatccc tgtcaatttc ttgttcttaa aatctttgtg tcttcgtagt gaaattttca
      541 gctaaagcgc gattcgcttg acgccgtaga tttttctatt agtatttccc tgcacaaaaa
      601 ccacgagctt caaattttcc tttttccagc ccggctgcgt ttgaaaagcg gtttccattt
      661 caaacgattt atcctgcgcg ctcaggtttc cgattgattt cagctcgcgc gcgaccgaaa
      721 cgtgttccag agttttcccc gaattttcgc cgcgcctgac tttggaaacc agattgtctt
      781 cggcaatcgc cagaaacacg ctcgcgtttt cgtgttcggg cattttggag atttttattt
      841 ttaatttttc gccgtcaatc gacaattcaa tcgtcgcttt cttatctttt gccgcttcca
      901 gaatttcctt cgtcgcgcga ttgccgtcgc cgccggtaaa ctggacgctt ccgtcgacaa
      961 tcatctgcgg cgtgtaaacg tccggttgtt tgaatcgctg cgcgtagagc gactggcgct
     1021 ggctgaaaag cggcgacgaa aactcgtccg tccagccgag ccggttccag taatcgacgt
     1081 gcagcgcgag cgtaatgatt tcggcttgcg agacgggctg ctgtttttcg aaaaaggcta
     1141 acgcacggtc agccggcggg cagctgcttc agccttcgct ggtgtaaagc tcgaccagaa
     1201 ccggcgtttt ttccatcgcg agcggagatg tttcattgcc gacatttcta ttttccgccg
     1261 tcagaaccgc attttccata ttgcaggcgg ctaagccgaa acacgtcgcg agcagcaaaa
     1321 acaaagtcat tttcataaat aaattatacc tgaaatttac gaggtttatt cccgctttga
     1381 gtctttgatg cggtttgtta aaatactctt ttgccttttt gccgaaattc ttaactatgc
     1441 aagctagcgt taaaacaaaa gaagtaatcg aagttcgcgg ggcgcgcgtt cacaatctga
     1501 aaaatatctc attttcgatt cccgtcaatg aactcacggt cgtcaccggc gtctccggct
     1561 cgggcaaatc ctcgctcgcc ttcgatacgg tttacgccga aggtcagcga agatatgtcg
     1621 aatcgctgtc ggcttacgcg cggcaatttc tggaaagaat ggacaagccg gacgtcgacg
     1681 aagttatcgg catcgctccg gcgattgcca tccggcaaaa aaattcgacg cgcaacccgc
     1741 gctcgaccgt ggcgacacaa accgagattt acgattacct gcgcctgtta ttcgcgcgcg
     1801 tcgggcagac tttctgtcac gtttgcgggc gcgaggtgaa aaaagattcg cccgaatcgg
     1861 cggcggagga aactctgaaa acgctggcgg aaggcgcgcg tttttacgtt atttttccgg
     1921 catccgacgc ggatttaagc tacgagacga aatcgaaggt cacggcgcat cttttaagcc
     1981 tgatgcagaa aggctttacg cgtctttttc gcggcggcga aacgctcgag ctgcaaaaac
     2041 cggaagatta taatttcagc gattttgacg acactttcgt tttgattgac cgcttgaagg
     2101 cggacgaatc catcaggcag cgtctggtcg attcgctcga aatctgtttt cgcgaaagcc
     2161 gcacggcga
//
LOCUS       DASWNE010000870         6474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_522307, whole genome shotgun sequence.
ACCESSION   DASWNE010000870 DASWNE010000000
VERSION     DASWNE010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6474
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522307"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VGB68_16990"
     CDS             complement(<1..531)
                     /locus_tag="VGB68_16990"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0050992.1"
                     /translation="MSPDKWKRLEEIFNQAVVLPTEKRQTFLDEACRGDRELREQADI
                     LLDSDSEAEDFIESSPYVSYLENVVEEKNGNNGGDSTIGKKIGVYRITKEIGRGGMGA
                     VYLAVRDDREFQQNVAIKLIKRGMDSDQVLRRFRNERQILAALNHPNIARLLDGGTTD
                     DGLPYFVMEYIEGLPVT"
     gene            1115..1693
                     /locus_tag="VGB68_16995"
     CDS             1115..1693
                     /locus_tag="VGB68_16995"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0050993.1"
                     /translation="MSAEKTQEITQLLIKLSNGQRAAVDDLLPLIYDELRSLASNYLR
                     RERVSHTLQPTALVHEAYLKLVDVNQVTWQNRAHFFGVAANLMRRILIDYARQHKADK
                     RGGEFQKLALDENIDKAAEQSTELVALDDALETLAKIDPVKSRIVELRYFGGLTVEET
                     AEVMGISTITVKRHWRMAKAWLYGQLSNDDAD"
     gene            1677..4346
                     /locus_tag="VGB68_17000"
     CDS             1677..4346
                     /locus_tag="VGB68_17000"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0050994.1"
                     /translation="MTTPISATRWQKIEDIFQTALDKPTPAKREAYLAEACAGDASLR
                     AEVEKLLAQFDEAEDYAELDETEPDNRSLLSTLLSTDKDDEDPVIGNRLGAYRVEAEI
                     GRGGMGTVYSAVRDDREFNKKVAVKLVKRGMDTDYILRRFRKERQILASLEHPFIAAL
                     LDGGTSPDGRPYFVMELVEGKNFYEYCDDNRLNLRERLRLFLKIAESVNFAHQNFVIH
                     RDLKPSNILITAQGLPKLLDFGIAKLLNPENPNEAVSQTFTLMRAMTPKYASPEQIKG
                     DALTFATDVYSLGVLLYEIFCGHRPYVPAGAQAHELARVICEEIPELPSAAIARNDAG
                     VLDEYKNKPGSPVDLIAEKRASSLEQLQKELTGALDSIVMKCLEKRPRKRYAGVRDLI
                     ADIDAYLTGNALNIPFYTPPSGQDTSTTTGGNAVAVLPLKLMGKGDGDYLSLGLADAL
                     ISRLSQVKRFTVRPTSSVLRYAQEETFDPLQAGRELNVNYVLDGWIKQFGGRIRISLQ
                     LLEVREGNVVWAGKFDEKSGDVLEIEDSISAQVGEALVPQLSGDEREKLARRPTDNAA
                     AFEAFLRGRYHFNTFTEEGFAKAIAAYNQAIELDPGYALAYAGIADYYNWLGVYGVLP
                     ARECFQAAKNNSLKAIELNENLSEAHAALGFAAVAGDYDWKRGEQACRRALELNPHNA
                     QAHVWYSLQFFMEARFDEGEHHARRGVELDPLTPFNLYNLAWCLYFARRYEDSIRQYE
                     KTIAENPLYSIAYYGLSWTLRKIGRYEEALNASRRALELSGEDSLFNLAQYGQTLACA
                     GRREEALEVLARLDAASAERFVSPYHTAIIYCFLGDKEKTFEYLERSFAEKEAWLVWT
                     GVEPIFDEIRADRRFKDILRRINYPRLSERRAS"
     gene            complement(4564..4824)
                     /locus_tag="VGB68_17005"
     CDS             complement(4564..4824)
                     /locus_tag="VGB68_17005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050995.1"
                     /translation="MKFVFNGIVNVTGEGDDMTFEFIHQNDAQRINALGLDNNQLYGS
                     CRIGMSGSDPGNFYCVDDGCDNGECQLRNSGSSYWCSCVVAA"
     gene            complement(4966..5322)
                     /locus_tag="VGB68_17010"
     CDS             complement(4966..5322)
                     /locus_tag="VGB68_17010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0050996.1"
                     /translation="MKIKLNFIVKILAVLMIIGMMDSAAFSQTRLRFARGRTSTTVSG
                     KISSGSSRSYILGASRGQTMTVGVRSGNGDVHLDIGGNDVGTGTTIQLRSTDDYIITV
                     HNEGGATGYSLYVSIR"
     gene            complement(5547..6071)
                     /locus_tag="VGB68_17015"
     CDS             complement(5547..6071)
                     /locus_tag="VGB68_17015"
                     /inference="COORDINATES: protein motif:HMM:NF020177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthrone oxygenase family protein"
                     /protein_id="HEY0050997.1"
                     /translation="MTRQFSIAQIFLWLFVITLGITIGGGLYETLVVMPLWTAAPPES
                     VIAYHRHNVANPQFTLDQGGRFWIFLTPLLALLTIAALLSGLRTRPEHRKWRMTATVL
                     ALVVVISTFAWFIPNIIKLTGEGVTTMNPDQITSLTNWWSRLNWLRAILYFAAWLAAL
                     RALSISPKMERLYN"
BASE COUNT         1559 a         1762 c         1657 g         1496 t
ORIGIN      
        1 cgtcaccggc aagccttcga tgtattccat cacgaaatac ggcaaaccgt cgtcggtcgt
       61 gccgccgtca agcagacgcg cgatattcgg atgatttaag gcggctaaga tttgccgctc
      121 gttgcggaag cggcgcaaaa cctggtcgct gtccattccg cgcttgatta atttaatcgc
      181 gacgttttgc tgaaactcgc ggtcgtcgcg gacggcgaga taaaccgcgc ccataccgcc
      241 gcgcccgatt tccttggtga tgcggtaaac gccgattttt ttgccgatag tcgaatcgcc
      301 gccgttattg ccgttttttt cctcgacgac gttttccaga taagatacgt agggcgaaga
      361 ttcgataaaa tcttcggctt ccgaatcgga atcgagcaga atatcggctt gctcgcgcag
      421 ctcgcggtcg ccccggcaag cttcgtcgag aaaagtctgg cgtttttcgg tcggaagaac
      481 aaccgcttga ttaaagattt cttcaagcct cttccattta tcgggtgaca tctaattata
      541 gttttgcggt ttaaaaagct cgcctcttat ccgggctaaa cggcactgac gaatcgacag
      601 tgaagcgaaa ttttaacata ctttccagca agtaatctaa gaagcagatt aaacctgcgg
      661 cgttgtccga aactaaagca atcttttgca aataaaaaca cttccgatta aataatttag
      721 ccgcgcgcga gcgccgtaaa tcagacgctg aaaccggcaa aaattgcgaa gaaatccagt
      781 agccgggagc gctgcggaaa atccggcaaa tgaaaccgcg catacgcgcc gcgacgaacg
      841 cggaacgatg aataacgaat tgcgggttcc gcgctttgcc tatccgcgct cattatttct
      901 tatcccgcgg cgtcaagccg cgaagcccgc tgcgccgggt ttgaatccgc ctttatccgt
      961 gtaaatccgc ggtttcattt gctgcggatt gtctccggcg ctgccgacgt ctgaaatctt
     1021 tcattttcaa taacaatttg gaaatgtcgc gtcgtctcgt ttatactctg ctttgacctt
     1081 ttaaaacaaa gattcggaga ggttcaagaa agccgtgtcc gccgaaaaaa cacaagaaat
     1141 aacgcagtta ttgataaagc tttccaacgg tcagcgcgcc gcggttgacg accttttgcc
     1201 gttgatttac gacgaattgc gcagtctcgc ctcgaattac ctgcggcgcg agcgcgtctc
     1261 gcatactttg cagccgacgg cgctcgtcca cgaagcgtat ctgaagctgg tcgacgtcaa
     1321 tcaggtgacg tggcagaatc gcgcgcattt tttcggcgtc gcggcaaatc tgatgcggcg
     1381 catcctgatt gattacgccc gccagcacaa agccgacaag cgcggcggcg aatttcaaaa
     1441 actcgcgctg gacgaaaaca tcgacaaagc cgccgagcaa agcacggagc tcgtcgcgct
     1501 ggacgacgcg ctcgaaactc tcgcaaaaat cgaccccgtc aaaagccgca tcgtcgagct
     1561 gcgctatttc ggcggcttga ccgtcgaaga aaccgccgaa gtgatgggca tctccaccat
     1621 taccgtcaag cgtcactggc gaatggcgaa ggcttggctc tacggacaac tcagcaatga
     1681 cgacgccgat tagcgcgacg cgctggcaaa aaatcgaaga catttttcag acggcgctcg
     1741 ataaaccgac gcccgccaaa cgcgaagcgt atctcgccga agcctgcgcc ggcgacgcga
     1801 gcctgcgcgc cgaagtcgaa aaactgctcg ctcaatttga cgaagccgaa gattacgccg
     1861 agctcgatga aaccgagccc gataatcgct cgcttttatc gactttgctg agcacagata
     1921 aagacgacga agacccggtt atcggcaatc ggctcggcgc gtatcgcgtc gaggcggaaa
     1981 tcgggcgcgg cggtatggga acggtttact cggcagtccg cgacgaccgc gagtttaata
     2041 aaaaggtcgc cgtcaaactg gtcaaacgcg gaatggacac cgattatatc ctgcgccgct
     2101 ttcgcaagga acgacagatt ttagcgtcgc tcgaacatcc gtttatcgcc gcgctgctcg
     2161 acggcggaac gtcgcccgac ggtcgcccgt attttgtgat ggagctggtc gagggcaaaa
     2221 atttttacga atactgcgac gacaatcgct tgaacctgag agaaaggctc agactctttt
     2281 taaaaatcgc cgaatcggtc aatttcgcgc atcagaattt tgtcattcac cgcgatttaa
     2341 agccctcgaa tattcttatc acggcgcagg gcttgccgaa acttctggat ttcggcatcg
     2401 ccaagctttt aaatcccgag aatccgaacg aagccgtctc gcagactttc acattaatgc
     2461 gcgcgatgac gccgaaatac gcctcgcccg aacaaatcaa aggcgacgcg ctgacatttg
     2521 cgaccgacgt ttacagcctc ggcgttttgc tttacgagat tttctgcggt catcgcccgt
     2581 atgttccggc tggcgcgcag gcgcacgagc tggcgcgcgt catctgcgag gaaattcccg
     2641 agctgccgag cgccgcgatt gcgcgaaacg acgccggcgt gctggacgaa tacaaaaaca
     2701 aaccgggttc gcccgtcgat ttaatcgccg aaaaacgcgc gtccagcctt gagcagctgc
     2761 aaaaggaatt aacgggcgcg ctcgacagca tcgtgatgaa atgcctggaa aaacgcccgc
     2821 gcaagcgtta cgccggcgtc agggatttaa tcgccgacat cgacgcttat ctgaccggaa
     2881 acgctttaaa tattccgttt tacacgccgc cttccggaca ggacacatcg accacgacgg
     2941 gcggaaacgc ggtcgccgtg ctgccactga aactgatggg caaaggcgac ggcgattatt
     3001 tgagcctcgg tttggctgac gctctgattt cgcgtctgag ccaggtgaaa agattcaccg
     3061 tgcgcccgac aagctccgtt ttgcgttacg cgcaggaaga aactttcgac cctctgcaag
     3121 ccgggcgcga gcttaatgta aattacgttc tggacggctg gataaaacaa ttcggcgggc
     3181 gcattcgcat ctcgctgcag cttctggaag tgcgcgaagg caatgtcgtc tgggcgggca
     3241 aattcgacga gaaatccggc gacgtgctgg aaatcgaaga ctcgatttcg gcgcaggtcg
     3301 gcgaagcctt agtgccgcag ctgtcgggcg acgagcgcga aaagctcgcc cggcgaccga
     3361 ccgacaacgc cgccgctttc gaagcttttc tgcgcggtcg ctatcatttc aacactttta
     3421 cggaagaagg tttcgccaaa gcgattgccg cctacaatca ggctatcgag ctcgacccgg
     3481 gttacgcgct cgcctacgcc ggcatcgccg attattacaa ctggctcggc gtctacggcg
     3541 ttttgcccgc gcgcgagtgt tttcaggcgg cgaaaaataa ttcgctgaaa gccatcgaat
     3601 taaacgaaaa tctctcggaa gcgcacgccg cgctcggttt cgccgccgtc gcgggcgatt
     3661 acgactggaa acgcggcgag caagcctgcc gccgcgcgct cgagctgaac ccgcacaacg
     3721 cgcaggcgca cgtctggtat tcgctccagt tttttatgga agcgcgtttc gacgagggcg
     3781 agcatcacgc ccgccgcggc gtcgagctcg acccgctgac gccgtttaac ctgtataatc
     3841 tggcgtggtg tctgtatttc gcgcgccgtt atgaagacag catccggcaa tacgaaaaaa
     3901 cgattgccga gaacccgctc tacagcatcg cttattacgg cttgagctgg acgctgcgaa
     3961 aaatcgggcg ttacgaggaa gccttaaacg cctcgcgccg cgcgctggaa ttatccggcg
     4021 aagacagcct tttcaatctc gcccaatacg gtcaaacgct cgcctgcgcc ggacggcgcg
     4081 aagaagcgct ggaagttctg gcgcggctcg acgccgcttc cgccgaacgc ttcgtttcgc
     4141 cctatcacac cgccatcatc tattgctttc tcggcgataa agaaaaaact ttcgaatatc
     4201 tggaacgctc gtttgcggaa aaagaagcgt ggctcgtctg gacgggcgtc gagccgattt
     4261 tcgacgaaat ccgcgccgac cggagattta aagacatcct gcgacgcatt aattatccga
     4321 ggctttcgga aagacgggca tcataaattc cggcgcgctt tgccggacaa agctttccgt
     4381 cgcgttcata tttctcctcg acgagaaagc aaggcagcgg tttgcctgcc gccgccgaac
     4441 aggcgctgag cggcaagcca atcgctttga ccgattaatt taattggttc tccgaccgaa
     4501 atatccggaa gctatttcaa aaatggtttc ttaacaaaat aaagattccg gttttgtaat
     4561 ctttcaggcg gcaaccacgc agctgcacca ataactcgac ccggagtttc ttaactggca
     4621 ctctccgtta tcgcatccgt cgtcaacgca ataaaaattg ccgggatcgc tgccgctcat
     4681 accgattcga caactaccgt acaactggtt attgtcgaga cccaaagcgt taattcgctg
     4741 agcgtcattc tgatgaataa attcaaaggt catatcgtcg ccttctcccg ttacattaac
     4801 aatgccatta aaaacgaact tcataatttt tttctcctcg tttgaatttt acgcagccta
     4861 taaaggcggc ttaccctttt ttaccaataa actcggcgaa agctgtaaag ctcaccgacc
     4921 ttaatagtta tctttttaca atttcgcttt cgatgccggt aaaagtcagc gaatcgaaac
     4981 ataaagcgaa tagccggtcg ctccgccttc gttgtgaacc gtaataatat aatcatcggt
     5041 cgaacgcaat tgaatcgtag tgccggtgcc gacatcgttg ccgccgatgt ctaaatgaac
     5101 atcgccgttt ccggacctga cgccgacggt catcgtctgc ccgcgggacg cgcctaaaat
     5161 atagctgcgg ctgctgcccg aactgatttt ccccgaaacg gtcgtcgatg tccgaccgcg
     5221 cgcaaagcga agcctcgtct gcgaaaaagc cgccgaatcc atcatcccga ttatcatcaa
     5281 aaccgccaga attttaacga taaaatttag ttttattttc actgtcgttc tcctttgtcg
     5341 ttttactgaa atgctttttt atatatgcga aattttccat caggaaaggt caaattattt
     5401 tcaccgcgca aagttccgct acaatgatgt ttttttccgc cgcagagcca taacgccaat
     5461 gtataaatcg ttccctgccg agtttttgca cgcgcgcttt ttgcgttcga ttgtttatcc
     5521 ggctgtgcgt tgattaaggt gtggattcag ttataaagtc tctccatttt cggcgaaatg
     5581 ctcaatgctc gcagggcggc gagccaggcg gcgaaatata ggattgcgcg cagccagttc
     5641 agccgcgacc accagtttgt caggctggtt atctggtcgg gattcattgt tgtgacgcct
     5701 tcgccggtta gtttgatgat gttcgggata aaccaggcaa atgtggagat gacgacgacg
     5761 agcgccagaa cggtcgccgt catccgccat ttgcgatgtt cggggcgcgt tctcaaaccc
     5821 gaaagcaaag cggcgatggt cagcaaggcg agcagcggcg tcaggaaaat ccagaatctg
     5881 ccgccctgat cgagcgtaaa ctgcgggttg gcgacgttat gccggtgata ggcgataacc
     5941 gattccggcg gcgcggcagt ccacagaggc atcacgacca gcgtctcgta taaaccgccg
     6001 ccgatcgtga ttcccagcgt aatcacgaaa agccatagaa aaatttgggc gattgaaaac
     6061 tgtcttgtca tttatttctt ctcatttccg ttcggaaaaa cgtataaaac gtagccgccg
     6121 gattttgatt cgcactgaat tgtttttatt ggaagcagac ttttatatcg ttaacagtct
     6181 gataagtcaa gaaaaactcg ttttattgcc gaatttagcg aatattcgcc gaatcgggaa
     6241 attgctttcg taaattcctc gaaactcttt tagccagccg ccgtctattg ttttttgcct
     6301 gcaagcgcgg ttgatttctt ctatctttcg gcaggcgggt ttagcataga aaaagccgcg
     6361 ttctcttaaa catttaagag aacgcggctt tagaacagaa cacttttaat ttactacgga
     6421 gtcgttccgc cccggacgga aatgttgccc gattcgatgg tttccagagg gaaa
//
LOCUS       DASWNE010000871         2690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_624891, whole genome shotgun sequence.
ACCESSION   DASWNE010000871 DASWNE010000000
VERSION     DASWNE010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624891"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VGB68_17020"
     CDS             complement(<1..615)
                     /locus_tag="VGB68_17020"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0050998.1"
                     /translation="MKYRKLGSSDLEVSAVGLGCMGMSDFYSGRDDAESLATIHRALE
                     LGVNFLDTADMYGPFTNEELVGKAIKGRRDDFVIATKFGIVRDSNDASFRGISGKPDY
                     VRQAVEGSLKRLGIETIDLYYQHRVDPETPIEETVEAMAKLVKEGKVRYLGLSEASAE
                     TLRRADAVHKITALQSEYSLWTRDVEDNDVLAACRDLGIGFVAYS"
     gene            complement(619..1527)
                     /locus_tag="VGB68_17025"
     CDS             complement(619..1527)
                     /locus_tag="VGB68_17025"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0050999.1"
                     /translation="MKVFLTGASGYVGAVIAEKLAGKGYKVTGLARDDAAEAKLRERK
                     ITPTRGELENFETLKKAAGEADAVIHTAFSHNFSDYNDAAKLDRNVIAAFAESLSRTN
                     KPFIVTSSSAVLGDTKGNEADEDYPFDQNSTRLLRGEAERDVLQLASKGIRGIVLRLP
                     LFVYGRGGSSFVPFMIRQARAAGAARYVETGEQKVSAVHVEDAADLYALALETSTAKG
                     LYNVAAESVSLKDLNESIARLLNVKAQSISKEKAKEAFGKMFDFLSINNRLSAERAQR
                     GLGWRANANKTILDDIENGSYRNFKE"
     gene            complement(1549..2190)
                     /locus_tag="VGB68_17030"
     CDS             complement(1549..2190)
                     /locus_tag="VGB68_17030"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY0051000.1"
                     /translation="MKIGIIGAGHIGANAARLFVEAGHEIAIANSRGAETLQDLVAEL
                     GERAQAVNAEEAAKFGEIVFLSIPFGKYKDLPADAFDGKIVIDSNNYYPDRDGSFAEL
                     DKNETTSSELLAEHLGSGARVVKGFNTIWFEHLKTQGDKSLPPEERRAIFIAGDDAAA
                     KETVAKLIADIGFAAVDTGDLADGGLAQQPGTALYNRNLTAGEAQAILGSEGY"
     gene            complement(2210..>2690)
                     /locus_tag="VGB68_17035"
     CDS             complement(2210..>2690)
                     /locus_tag="VGB68_17035"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY0051001.1"
                     /translation="LKGLKVIGTASSEQKLELVTKLGADAGVNYTESDWTDEVLRATE
                     GKGADWIIEMVGGEIVGQNLKVLAKNGTMWIYGAANGEDFPLSVLSLMQKNHIVRGYW
                     LRNETTENRIAFTRELQGYIRDGRLQIQVTEFPLEQAAEAHKAIEGRKTTGKVVLTV"
BASE COUNT          535 a          832 c          629 g          694 t
ORIGIN      
        1 ggaataagcg acgaaaccga ttcccaaatc gcggcacgcc gccagaacgt cgttgtcttc
       61 gacgtcgcgc gtccacaatg aatattcgct ctgcaaagcc gtaattttat gaaccgcatc
      121 ggcgcggcgc agagtttccg ccgacgcttc cgacaagccc aggtagcgaa cttttccttc
      181 tttgaccagt ttcgccatcg cttcgacggt ttcctctatc ggcgtttccg ggtcgacgcg
      241 gtgctggtaa taaaggtcta tcgtttcgat tccgagccgc ttcagactgc cttcgaccgc
      301 ctgcctgacg taatccggct ttccgctaat gccgcgaaat gacgcatcat tcgagtcgcg
      361 gacgatgccg aattttgtcg caatcacaaa atcgtcgcgc ctgcctttaa tcgccttccc
      421 aaccagctct tcattcgtaa aaggtccata catatccgcc gtgtcgagaa aatttacgcc
      481 cagttccagc gcgcggtgga ttgtggcgag agattccgcg tcgtcgcgtc ccgaataaaa
      541 atccgacatt cccatacagc ccagaccgac ggctgaaact tccaaatcgc tgcttcccaa
      601 ttttcgatat ttcatttatt actccttaaa attccggtac gacccgtttt cgatgtcgtc
      661 taaaatcgtt ttattggcat ttgcccgcca gcccagcccg cgctgcgctc tttcggctga
      721 aagccggttg ttaatcgaaa gaaaatcgaa cattttccca aaagcttcct tcgctttttc
      781 ctttgaaatg ctttgcgctt tcacatttaa aagccgcgct atcgattcgt tcaaatcttt
      841 gagcgagacg ctttccgccg cgacgttata gagccctttc gccgtgcttg tttccagcgc
      901 cagagcgtat aaatcggcgg cgtcctcgac gtgaaccgcc gaaactttct gctcgcccgt
      961 ctcgacgtaa cgcgccgcgc ccgccgcccg cgcctgccgg atcataaacg gcacgaacga
     1021 gctgccgccg cgcccgtaaa cgaaaagcgg gagccgcaga acgatgccgc ggatgccttt
     1081 tgacgctaac tgcaagacgt cgcgctcggc ttcgccgcgc agcaatctgg tcgaattctg
     1141 gtcgaaagga taatcttcgt ccgcttcgtt gcctttcgtg tcgcctaaga cagccgagct
     1201 ggaagtgacg ataaaaggct tgttcgtccg cgacaggctt tcggcaaaag cggcaatcac
     1261 gtttctgtcc agttttgccg cgtcgttgta atcggaaaaa ttatgactga acgcggtatg
     1321 aataaccgcg tcggcttcgc ccgcggcttt tttcaaagtt tcaaaatttt ccagctcgcc
     1381 gcgcgtgggc gtgatttttc gctcgcgcag tttcgcttcc gccgcgtcat cgcgcgccaa
     1441 acccgtcact ttataacctt ttccggctaa tttttccgcg atgaccgcgc cgacatagcc
     1501 gctcgcgccc gtcaagaaaa ctttcatatt tccgctcaat cttctttatc aatatccttc
     1561 cgagcctaaa atcgcctgcg cctcgcccgc cgtcagattg cggttataaa gcgccgttcc
     1621 gggctgttgc gccagaccgc cgtccgccaa atcgcccgta tcgaccgccg caaaaccgat
     1681 gtccgcaatc aatttcgcca ccgtttcctt cgccgccgcg tcgtctccgg cgataaaaat
     1741 cgcgcggcgt tcttcgggcg gcaaactttt atcgccctgc gttttcagat gctcgaacca
     1801 aatcgtgtta aagcctttga cgacgcgcgc gccgctgccg agatgctccg ccagcagctc
     1861 gctcgaagtc gtttcgtttt tatcgagctc ggcaaagctg ccgtcgcggt cgggataata
     1921 attgtttgaa tcaatcacga ttttgccgtc gaaagcgtcc gccggaaggt ctttgtattt
     1981 cccgaaaggt atggagagaa aaacgatttc gccgaatttt gccgcttctt ccgcgttaac
     2041 ggcttgcgcc cgctcgccta actcggcaac caaatcctgc aaagtttccg cgccgcgcga
     2101 atttgcgatg gcgatttcgt gtccggcttc gacaaaaagc cgagccgcgt ttgcgccgat
     2161 gtgtcccgcg ccgataattc cgattttcat ttgtcttttc tcccgtttct caaaccgtca
     2221 atacgacttt acccgtcgtt tttctgcctt cgattgcctt atgcgcttca gccgcctgtt
     2281 cgagcggaaa ttccgtcacc tgaatctgaa ggcgtccgtc gcgaatgtat ccctgcaatt
     2341 ctctggtgaa agcgattcga ttttcagtcg tttcgtttct caaccagtag ccgcgcacaa
     2401 tatgattttt ctgcatcagg ctcagtaccg acaggggaaa atcttcgccg ttcgccgcgc
     2461 cgtaaatcca catcgtgccg tttttcgcca gcactttcag attctgcccg acgatttcgc
     2521 cgccgaccat ttcgataatc caatccgcgc ctttgccttc ggttgcgcgc aaaacctcgt
     2581 cggtccagtc gctttccgtg taatttacac ccgcgtccgc gccgagttta gtgaccagtt
     2641 ccagcttctg ctcgctcgaa gcggtgccga taacctttaa gcctttcagc
//
LOCUS       DASWNE010000872         1511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_624949, whole genome shotgun sequence.
ACCESSION   DASWNE010000872 DASWNE010000000
VERSION     DASWNE010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624949"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VGB68_17040"
     CDS             complement(<1..536)
                     /locus_tag="VGB68_17040"
                     /inference="COORDINATES: protein motif:HMM:NF036634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051002.1"
                     /translation="MKRTIKAPTGTELTCKNWLSEAAYRMIQNNLDADVAFDPDNLIV
                     YGGRGKAARNWECFDAILDSLKNLEADETLLVQSGKPVAVFKTHTDAPKVLLANSNIV
                     PAWATQENFDKWERAGLIMYGQMTAGSWIYIGTQGILQGTYETFGSLARQNNWGTLAG
                     KFVLTAGLGEMGGAQAQA"
     gene            602..>1511
                     /locus_tag="VGB68_17045"
     CDS             602..>1511
                     /locus_tag="VGB68_17045"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HEY0051003.1"
                     /translation="MSNIFELVERPDEEIFFRRNDPNDVRLGEIVPNVRYEEAGVVIV
                     GCPEDEGVRRNNGREGTALAPDAIRREFYRFTPFGIGAKIFDAGNIKVEGSLEETHDA
                     LQTVASQILKDGKKIISLGGGNDISYPAGAAMAETFGAGNWIATNVDSHFDVRADRER
                     NSETAYRQLLEEKLLRSDYLYEVAYQTQLASPIYYRYLQNLNVNLISLEQLRSRTEAD
                     YEVRELMKQKFINHSSSLNSFFGFDLSAVRMSDAPGVTTPNPTGLRAGELITLVKFAA
                     SLVNTRIIGFTEVNPKFDIDNRTVKLV"
BASE COUNT          360 a          400 c          383 g          368 t
ORIGIN      
        1 atcgcctgcg cctgagcgcc gcccatttcg cctaaacccg ctgttaaaac aaactttccg
       61 gcgagcgttc cccaattatt ttgtctggct aggcttccga aagtttcgta agttccctgc
      121 aaaatcccct gcgtgccgat ataaatccag cttccggcgg tcatctgtcc gtacattatc
      181 agccccgcgc gctcccattt gtcgaaattt tcctgtgtcg cccaggcggg aacgatgttt
      241 gagtttgcca gcagaacttt cggcgcgtcc gtatgcgtct tgaaaacggc gaccggcttg
      301 cccgactgca cgagaagcgt ttcgtcggct tcgagatttt tcaagctgtc cagaatcgcg
      361 tcgaaacatt cccaattccg cgccgctttg ccgcgcccgc cgtagacgat taaattatcg
      421 gggtcaaacg ccacatccgc gtccagattg ttctgaatca ttcgataagc ggcttccgac
      481 agccagtttt tacaggtcag ctccgttccg gtcggggctt tgatagttcg tttcatattt
      541 ttctatttat aatgacgaat ggttttaaat atgatagttt tagactatca atcaagcaac
      601 tatgagcaat atttttgaat tggtcgagcg accggacgaa gagatttttt ttagacgcaa
      661 cgacccgaac gacgtgcggc tcggcgaaat cgttccgaat gtcagatacg aagaagcggg
      721 cgtcgttatc gtcggctgcc cggaagatga aggcgtaagg cgcaataacg gacgcgaagg
      781 cacggcgttg gcgcccgacg cgattcgccg cgagttttac agattcacgc cgttcggcat
      841 cggcgcgaag attttcgacg cgggaaatat aaaggtcgaa ggctctctgg aagaaacgca
      901 cgacgcgctt cagacggtcg ccagccaaat tctgaaagac ggcaaaaaga ttatcagtct
      961 cggcggcgga aacgatatat cttatccggc gggcgcggcg atggcggaaa ctttcggcgc
     1021 gggaaactgg attgcgacca acgtcgattc tcatttcgac gtgcgcgccg accgcgaaag
     1081 aaacagcgag acggcttatc gccaattgct cgaagaaaag cttctgcgct cggattatct
     1141 ttatgaagtc gcgtatcaaa cgcagcttgc ttcgcccatt tactaccgct atctgcaaaa
     1201 tctgaatgtg aatctcatca gcctcgaaca gcttcgctca cggacggaag ccgattatga
     1261 agtccgcgaa ttgatgaagc aaaaatttat caatcacagc tcttctttaa attctttctt
     1321 cggtttcgat ttgagcgccg tccgaatgtc ggacgcgccg ggcgtcacca cgccgaatcc
     1381 gaccggcttg cgcgccggcg aattgattac gctggtcaaa tttgcggcaa gtctggtaaa
     1441 cacccgaatc atcgggttta cagaggtcaa tccgaaattt gatatcgaca atcgcaccgt
     1501 aaaactggtt g
//
LOCUS       DASWNE010000873         3663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_625003, whole genome shotgun sequence.
ACCESSION   DASWNE010000873 DASWNE010000000
VERSION     DASWNE010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625003"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..2256
                     /locus_tag="VGB68_17050"
     CDS             244..2256
                     /locus_tag="VGB68_17050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051004.1"
                     /translation="MEKNEQSPADGFLKRWLLKGVKEVEGPHEPEGRHHQHPWWKVMC
                     LTGVDYFSTLGYQPGIAYLAAGALSPLATLVLVLLTLFGALPMYHRVAEESPHGDGSI
                     SMLENLLSRWKGKMFVLALLGFVATSFIITITLSAADATAHIIENPFVEHNLHFLNHR
                     IIVTLVLIGLLGAVFLKGFNEAIGLAVGIVIVYLTLNVVIIFYGLYQIFVVHPEALSN
                     WQGLLWTHPDVSGSVTWMAVAALVLFPKLALGLSGFETGVVVMPLIKSDNVSAPDAKN
                     IHTSQIDGQEMPAAEQKHLEGRIRNGKKLLTGAAVIMSFMLVGSSIITSTLIPPEQFQ
                     EGGEAYGRAISYIAHEYLGDIFGTFYDISTILILWFAGASAMAGLLNIVPRYLPRYGM
                     APDWARATRPLVLVFTVICFLVTILFRADVSAQGGAYATGVLMLMTSAAVAVTISAWK
                     RAEKKWIFFLLITLVFCYTTIVNMIEQPNGIKIASFFIIGIIFASFVSRAMRTTEVRV
                     EEIVLDEKAQEFLDELSGGEIRIVTNRRETGDIAEYRFKEHEKRVDNHIPSSDPILFY
                     EIELGDASEFTGKLHIRGVDVAGYKILRTQAPAVPNAIAGFLLYLRDKTGKIPHVYFG
                     WSEGNPIMYLARYILFGEGDTAPVTREILRQAEEDPERRPSVHVGG"
     gene            complement(2329..2715)
                     /locus_tag="VGB68_17055"
     CDS             complement(2329..2715)
                     /locus_tag="VGB68_17055"
                     /inference="COORDINATES: protein motif:HMM:NF024843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HEY0051005.1"
                     /translation="MSQFESRGLSGYEPLISAAEEHEIEDQSINCIDCGQDFTWTVGE
                     QVFFRDKGLKNPPKRCKPCKQAKNERLAAITAAQEAGIKQRIEVAVHCANCDAFTTVP
                     FYPSQGRPVFCRSCFLAMNPNLTAEK"
     gene            2960..>3663
                     /locus_tag="VGB68_17060"
     CDS             2960..>3663
                     /locus_tag="VGB68_17060"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:NF013254.4"
                     /note="catalyzes the formation of
                     N(1)-(5-phospho-D-ribosyl)glycinamide from
                     5-phospho-D-ribosylamine and glycine in purine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEY0051006.1"
                     /translation="MRILVVGSGGREHALCRAFKKSRRVTKIFCADGNAGIAEIAECV
                     GIKPDEIVRLADFAAEKEIDLTFVGGETALALGIVDEFEKRNLKIIGASRQAAQLEAS
                     KAFAKDFMFRQRIPTAGYEVADSTEKAVEILRNGKFGDENSPVVVKADGLAAGKGVVV
                     AKDRNEAAEAIDELENIVGKEAASKIVLEECLVGREVSLILFADGKNFALMPPARDHK
                     RIGENDTGANTGGMGV"
BASE COUNT          881 a          870 c          989 g          923 t
ORIGIN      
        1 acctaggggc gtcggtgtgg gagacgtcgc cctttaactt ttttatttgc cgattctacc
       61 gttttttttc attaaaactt gacctaaact ccaataagcg taaaatttag ctttgtaata
      121 tataaataaa ttgaaggctg accgtttttt attttactgt cagtgaaaaa ggtaaaaacg
      181 ttacaatgct tcgaaaagac cttagatacc gcacggtcgt ctaacggtct ttaagtattt
      241 tatatggaaa aaaatgaaca atctcccgca gacggttttt taaaacgctg gttgttaaaa
      301 ggcgtaaaag aagtcgaagg acctcatgag ccggaaggca ggcatcacca gcacccgtgg
      361 tggaaagtga tgtgtctgac gggcgtcgat tatttttcta cgctcggcta ccagccgggc
      421 atcgcgtatc tggcggcggg cgctctgtcg ccgctggcga cgctggtttt ggtgctgctg
      481 acgttgttcg gcgcgctgcc gatgtatcat cgcgtggcgg aagaaagccc gcacggcgac
      541 ggttcgattt cgatgctcga aaatctgctt tcgcgctgga aaggcaaaat gttcgtgctg
      601 gcattgctcg gcttcgtcgc tacgagcttt atcattacca tcacgctttc ggcggcggac
      661 gcgacggcgc acattatcga gaatcctttc gtcgagcaca atcttcattt tctcaatcac
      721 cgaatcatcg tcacgctggt tttaatcgga ttgctgggcg cggtattttt gaagggcttt
      781 aatgaagcca tcggtctggc ggtcggaatc gttatcgttt atctgacgct caacgtcgtg
      841 attatcttct acgggcttta tcaaatattc gtggttcatc ccgaagcttt gagcaactgg
      901 cagggtttgc tctggactca cccggacgtg agcggaagcg tgacctggat ggctgtcgcc
      961 gcgctggttc tttttccaaa actggcgctc gggctttccg gctttgaaac cggcgtggtc
     1021 gtgatgccgc tcatcaaaag cgacaatgtt tcagcgccgg acgcaaaaaa cattcacacc
     1081 tcgcagatag acgggcagga gatgccagcc gccgagcaga aacacctgga aggtcgcatc
     1141 cgcaacggca aaaaacttct gaccggcgcg gcggtcatta tgagctttat gctggtcggc
     1201 agcagcatta ttacctcgac gctgattccg cccgaacagt ttcaggaagg cggcgaagcc
     1261 tacgggcgcg ccatttccta tatcgcacac gaatatttag gcgatatttt cgggacgttt
     1321 tacgacatca gcacgatttt gattttgtgg tttgcgggcg cctcggcgat ggctggattg
     1381 ctgaatatcg tgccgcgcta tctgccgcgc tacggtatgg ctcccgattg ggcgcgagcc
     1441 acgcgtcctc tggtgctggt ttttacggtc atttgttttc tcgtcacgat attgtttcgt
     1501 gccgacgtca gcgcgcaggg cggcgcttac gcgaccggcg ttttaatgct gatgacttcg
     1561 gcggcggtgg cggtcacgat ttccgcctgg aaacgagccg aaaagaaatg gatatttttt
     1621 ctgttgatta cgctggtctt ttgttatacg accatcgtca atatgatcga gcagccgaac
     1681 ggtataaaaa tcgcttcgtt ctttatcatc gggattattt tcgcttcgtt cgtctcgcgc
     1741 gcgatgcgaa cgaccgaggt gcgcgtcgag gaaatcgttc tggacgagaa ggcgcaggaa
     1801 tttctggacg agctttccgg cggcgagata cggattgtga ccaaccggcg cgagacgggc
     1861 gatatcgcgg aatatcgctt taaggaacac gaaaagcgcg tggacaatca tattccgtcg
     1921 tcagacccga ttctttttta cgaaatcgaa ttgggcgacg cttccgagtt tacgggcaaa
     1981 ctccatattc gcggcgtcga tgtcgccggt tataaaattc tgcgcacgca agcgcccgcc
     2041 gtgccgaacg ccatcgccgg atttttgctt tacttaagag ataaaaccgg aaagattccg
     2101 cacgtttatt tcggttggag cgagggcaat ccgattatgt atctggcgag atatatcctg
     2161 ttcggcgaag gcgacaccgc gcccgtgacc agagaaattc tcagacaagc ggaagaagac
     2221 ccggaacgtc gtccgagtgt gcacgtcggc ggttaattgg atttaagatt caggattcag
     2281 gattactttc catttcaaat cctgaatctt aaatcttgaa ttctgagatt atttttcagc
     2341 ggtcaggttc ggattcatcg ctaaaaagca ggaacggcaa aatacggggc gtccctgtga
     2401 aggataaaaa ggaacggtcg tgaaagcgtc gcagttggcg cagtgaacgg cgacctcgat
     2461 gcgctgcttg atgcccgctt cctgcgcggc ggtgatagcg gcgagccgct cgtttttggc
     2521 ttgtttacag ggtttacagc gcttcggcgg attttttaag cctttatcgc ggaaaaaaac
     2581 ctgttcgccg acagtccagg taaaatcctg accgcagtcg atgcagttga ttgattggtc
     2641 ttcgatttcg tgttcttcgg cggcagagat taacggttca taaccgctta agcctctcga
     2701 ctcgaattgt gacattactt gttccctccc tttgcctcgt tcccaacaca attgagtttg
     2761 taataatcgg tggaagaccc agtttttcat cgggaaatga taaacttgtc tccgcaattc
     2821 tacataaaag attatattat aatttcaagg ttcttttgac aaaattcgca agtctcaaag
     2881 atttaacgag agtaggtata gacttgaagt tatacttatc cgagaaaagt gtcaaggaaa
     2941 ataaatttaa aacatcaaga tgagaatatt agtagtaggt tcgggcggac gcgaacacgc
     3001 tctttgccgc gcttttaaaa aaagtcggcg cgttacgaaa attttttgcg ccgacggtaa
     3061 tgccggaatc gccgagatag ccgagtgcgt cggcatcaaa ccggacgaaa tcgtcaggct
     3121 cgccgatttt gccgccgaaa aggaaatcga tttgacgttt gtcggcggcg aaacggctct
     3181 ggctttgggg attgtcgatg aatttgaaaa aagaaactta aaaatcatcg gggcgagccg
     3241 ccaggcggcg caactcgaag cgagcaaagc gtttgccaaa gattttatgt ttcgccaacg
     3301 gattccgacc gccggatacg aagtcgccga ttctacggaa aaagccgtcg agattctccg
     3361 aaacggaaaa ttcggcgatg aaaattcgcc ggtcgtcgtc aaagccgacg gcttggcggc
     3421 gggcaaaggc gtcgtcgtgg cgaaagatag aaacgaagcc gcagaggcaa tcgacgagct
     3481 ggaaaacatc gtcggaaaag aagccgccag caaaatcgtc ctcgaagaat gtctggtcgg
     3541 cagggaagtt tcgctgattt tgttcgccga cggtaaaaat ttcgcgctga tgccgcccgc
     3601 gcgcgaccac aagcgcatcg gcgaaaacga caccggcgca aacaccggcg gaatgggcgt
     3661 cat
//
LOCUS       DASWNE010000874         2113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_206775, whole genome shotgun sequence.
ACCESSION   DASWNE010000874 DASWNE010000000
VERSION     DASWNE010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206775"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VGB68_17065"
     CDS             <1..203
                     /locus_tag="VGB68_17065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051007.1"
                     /translation="LQPFISRHRRVQLVQSFFQSHKFFRLSPFISRLLKRKNAGKRLR
                     VSINKRLLGNNPFLFRIENKQI"
     gene            894..1940
                     /gene="mtnA"
                     /locus_tag="VGB68_17070"
     CDS             894..1940
                     /gene="mtnA"
                     /locus_tag="VGB68_17070"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEY0051008.1"
                     /translation="MIIPVKWSDEGVLMLDQRLLPTEEKWLTLRTYTEVAAAIRDMVV
                     RGAPAIGVSAAYGIALGVKNFVGTNLLDLEDDFDYIAETLGKTRPTAVNLFWAIDRMR
                     RTFQKAKSEGKSIGEIKQILLVDAKAIHEEDIESQRLIAQFGGELIEDNSTVLTHCNA
                     GALATGGVWGTALGVIRGAVNQGKHINVFADETRPYLQGARLTAWELLQDDIPVTLIT
                     DNMSGHIMKQGKIQAVVVGSDRIAANGDVANKIGTYMVAVLARRHNIPFYVAAPLSTV
                     DLNCPTGEQIPIEERNIREITHIQDIQLSPEGISVSNPAFDVTPNDLVSAIITEKGVA
                     RAPYTESLKKMFEE"
BASE COUNT          567 a          510 c          504 g          532 t
ORIGIN      
        1 atttgcagcc atttattagc cgtcaccgcc gagtgcagct tgtccagagt tttttccaaa
       61 gtcataaatt tttccgcctt tcgccattta tttcccgctt gttaaagcgt aagaacgccg
      121 gaaaacggct ccgtgtttca ataaataagc gtcttcttgg caacaatcca tttcttttta
      181 gaattgaaaa taaacaaata tagctggcgt tcggctattg tctgttgtat attgcttgca
      241 atttaatagt ctgaaatatg ccaacggctg cggctgctaa attttgacgt gaaacgagcg
      301 tttcccggta atctcgcaag catcgagagc caaattctaa tccgatgctc tcgcccgtta
      361 caaggtgatt aattagcgat taggacacca attacgactt ataaaggaga aaaaatgctt
      421 aaaaacgcgg taattctgaa acgaatatta atcctggcga tgttttcagc ttgtttaggc
      481 tggattagct ttgacaacac aaaacccgtt caggcagcag cttatgtttg ttgcagggat
      541 tgtaatgcga tgaccgaagc ttgtgcagca aattgtaata atcagacctg tatcgacaaa
      601 tgtctggaac aggaagaaac ctgttttgcg acgtgcacta acagcgttct tctttgccct
      661 taaagcgatg aggcagacag cgcgcatcat tttgaagtaa tcgagccgcc gtcaaaatgc
      721 acggcaaaaa tatcggcgta atcggcacgg caggagcgtg ctcgattatc ttgaatcgca
      781 gggtaattgt ccggcaatta ccttgttcgc ttttctgctg aaagctgcgc ggcgaatttg
      841 ttccttcctg taaaaaatat gtaataattg tttttagaag gaaaattaaa cgaatgataa
      901 tacctgtcaa atggagcgat gaaggcgttt tgatgctcga ccagcgtctt ttaccgaccg
      961 aagaaaaatg gctgacgctc agaacctata cggaagtcgc cgccgccatc cgcgatatgg
     1021 tcgtgcgcgg cgcgcccgca atcggcgttt ccgccgcgta cggcatcgcg ctcggcgtga
     1081 aaaattttgt cggcacgaat ctgctggatt tggaagacga ctttgattat atcgccgaga
     1141 ctctaggcaa aacgcgcccg accgccgtca atcttttctg ggcgattgac agaatgaggc
     1201 gcactttcca gaaagcgaaa tccgaaggca aatcaatcgg cgaaatcaaa cagattttgc
     1261 tggtcgatgc caaagccatt cacgaagaag acattgaaag ccaaagatta atcgcgcagt
     1321 tcggcggcga gcttatcgaa gataactcga ccgttctgac gcactgcaac gcgggcgcgc
     1381 tcgccaccgg cggcgtctgg ggaacggcgc tcggcgtcat tcgcggcgcg gtcaaccagg
     1441 gaaaacacat caacgttttc gccgacgaga cgcgcccgta cctgcaaggc gcgcgcctga
     1501 cggcttggga actgctgcag gacgatatcc cggtgacgtt gattaccgac aatatgagcg
     1561 gtcacattat gaaacaggga aaaattcagg cggtcgtcgt cggctcggat cgcatagccg
     1621 ccaacggcga cgtcgccaac aaaatcggaa cttatatggt cgcggttctg gcaagacgtc
     1681 acaacattcc gttttacgtc gccgcgccgc tttcgaccgt cgatttaaac tgcccgaccg
     1741 gcgagcagat tccgattgaa gaacgcaata tccgcgagat aacgcatatt caggacattc
     1801 agctttcgcc cgaaggcatc agcgtttcca atccggcgtt tgacgtgacg ccgaacgatt
     1861 tagtctcggc aatcatcacc gaaaaaggcg tcgcccgcgc gccttacacg gaaagcttaa
     1921 agaagatgtt tgaagaataa attacgaatt acgaattacg aatgaaggaa atattctttt
     1981 aattcgtaat tcgtaatttg taattcttaa ttgaaaaagc gcgctattcc gccgcgcttt
     2041 ttttaatatc cttttgcagc catttccacg ccgattcgat aatttccttt aaatcctgac
     2101 gttccggttt cca
//
LOCUS       DASWNE010000875         4768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_698856, whole genome shotgun sequence.
ACCESSION   DASWNE010000875 DASWNE010000000
VERSION     DASWNE010000875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4768
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698856"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VGB68_17075"
     CDS             <1..346
                     /locus_tag="VGB68_17075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0051009.1"
                     /translation="VEDYEDTRDLMKFLVESYGYQVIEAADGIEAVDKVKQLHPDLIL
                     MDISLPMVDGLTATKFIRQVDPLSKVPIIAVTAFGKSYYQKAIEAGCNDLIPKPLDFD
                     TLEPIINQYLAA"
     gene            complement(528..1127)
                     /locus_tag="VGB68_17080"
     CDS             complement(528..1127)
                     /locus_tag="VGB68_17080"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEY0051010.1"
                     /translation="MSIKVPRQFYTRDETLKVARDLLGKILVVPTESGERVSGMIVET
                     EAYLGAIDKAAHSYGNRRTPRTEMMFAEGGTVYIFFIYGMYFQFNIVAGAVDSPHAVL
                     IRAVAPVEGIEIMRERRGQMKDANLTSGPGKLCIAFGIDKTFNGENLLGERVWLEDGE
                     PFTDAQIASGKRIGIDYAQEFAEKPWRFWVKDNLFVSRK"
     gene            complement(1254..1607)
                     /locus_tag="VGB68_17085"
     CDS             complement(1254..1607)
                     /locus_tag="VGB68_17085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0051011.1"
                     /translation="MSELRFAGTVDHLAVKCEDLSKSVEEYKRLGFAVETVYEDWAMM
                     RDAKGFGIALLPPNSKHPPHIGMKVETLEELREAAARENRPIKPHRDGTSSFYTKGID
                     GNIVELIYYPEDSGK"
     gene            1740..2687
                     /locus_tag="VGB68_17090"
     CDS             1740..2687
                     /locus_tag="VGB68_17090"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEY0051012.1"
                     /translation="MRIASLLPSATEIVCALGLEENLIGITHECDYPPRVAGKPHLTA
                     SRISHETMSSAEIDHAVRSQLDGHGSIYDLNTALLAELKPDLIITQELCEVCAVSYKT
                     VERAARMYVAGAQVVSLEPNTIEDIFANIKTVGELCNVSEKAEEVVTNLRERLGRLRE
                     QSAAAETRPKVFMLEWLEPPFAPGHWVPEQTEAAGGISLLGKAGEKSVMTTYEKILET
                     APDRLVLIPCGYYAADILRQLPNTIFPESFRELPAVKTGEVWALDATAYFSRPAPRVI
                     DGAEILAKIVHPEIFGAPAETQAVRVSKNLLRFSPAVSN"
     gene            2831..4180
                     /locus_tag="VGB68_17095"
     CDS             2831..4180
                     /locus_tag="VGB68_17095"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY0051013.1"
                     /translation="MKILIALFYIAVCSLASLAQTNAEILASANGQKFTVADLPPDLR
                     EAYKQRNTKTAQLRQDILTQMIVDTLLESEAAARKTDVEKLEAEARAKAAAPTEAQIK
                     AVYNANKAKIGERTLEETREQIVAFLRRENEQKALVAFVKSLQAKYKIASLKPLNAPN
                     LKAADILVTVGAKKIAAGQFEQVARREIYEYRAEIYDAVVNTLEEVIYLKLVEAEAQK
                     SGIASGDLLAREITDKMRDFSNEESEKLRYDFRQSLFRKYNVQILVKEPEPFAQNIST
                     DGDPFQGKANAPVTVVMFSDFQCSVCAATHPILQKVLAGYGDKVRFVVRDFPLTPIHK
                     NAFLAAQAANAANAQGKFFEYTEILYRNQAALDADSLKKYAADSGLNVGQFELDLQSG
                     KFAGEVRRDMADGKRYGITGTPTIFINGVKVRVLSAKSFRDAIEKALAKTKVSAGSR"
     gene            4247..>4768
                     /locus_tag="VGB68_17100"
     CDS             4247..>4768
                     /locus_tag="VGB68_17100"
                     /inference="COORDINATES: protein motif:HMM:NF016952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016603 - glutaminyl-peptide
                     cyclotransferase activity [Evidence IEA];
                     GO_process: GO:0017186 - peptidyl-pyroglutamic acid
                     biosynthetic process, using glutaminyl-peptide
                     cyclotransferase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HEY0051014.1"
                     /translation="MIFAIFAFACGGETPPINANSTVKNTAGNNSLSPTSTAANNAAA
                     PPNVSTYEIVNTFRHDSKAFTQGLVFQNGFFYESTGEYGDSTLRKVEPQSGKVLQEHE
                     LAREYFAEGMTVLNGKIYQITWQENTAFVYDLENFKLLREIKYQGSGWGLTTDGTHLI
                     LSDGTHIIKFLDPE"
BASE COUNT         1227 a         1222 c         1208 g         1111 t
ORIGIN      
        1 tgttgaagat tatgaagata cacgcgattt aatgaaattt ctcgtggaaa gctatggtta
       61 tcaggttatc gaggcggcag acggaattga agcagtagat aaagttaagc agctccaccc
      121 tgaccttatc ctgatggata tttcccttcc gatggtcgac ggcttgacgg cgacaaagtt
      181 tatcagacag gtcgatcctt tatctaaagt gccgattatc gcggttaccg cattcggtaa
      241 atcgtattat caaaaggcaa tcgaagcggg ctgtaatgat ttaataccca aaccacttga
      301 ctttgataca ctggagccga ttatcaatca gtatctggcg gcgtagccga aagaaagttt
      361 taaacaaaat aaaaaagtcg tccgtgtgaa aatacacgga cgactttttt attttgttta
      421 aatgctttag taatcgtccc gatacggaca aataccggaa atgtgaaaaa tcgcgccttt
      481 gttccggcat ccgtcgcctg acggacgata aattatcatc cgtaaaatta ttttcggctg
      541 acaaacaaat tatctttcac ccagaagcgc cagggctttt cggcaaattc ctgcgcataa
      601 tcgatgccga tgcgctttcc gctggcaatt tgcgcgtcgg taaacggctc gccgtcttcc
      661 agccagactc tttcgccgag caaattctcg ccgttaaagg ttttatcgat gccgaaagcg
      721 atgcagagct ttccgggacc ggaggtgaga ttcgcgtctt tcatctgtcc gcgccgctcg
      781 cgcataatct ctatgccttc gacgggcgcg acggcgcgaa tcaaaaccgc gtgcggcgaa
      841 tcgaccgcgc ccgcgacgat attaaactgg aaatacatcc cgtaaataaa aaaaatgtaa
      901 accgtcccgc cctcggcaaa catcatctcg gtgcgcggcg tccggcgatt tccgtaagaa
      961 tgcgcggctt tgtcaatcgc gccgagatag gcttccgttt cgacaatcat tcccgaaact
     1021 ctttcgccgc tttcggtcgg aacgacgagg atttttccga gtaaatcacg cgcgactttg
     1081 agcgtctcgt ctcgcgtgta aaattggcgc ggcactttta ttgacataga gaaatttaac
     1141 aggatggaca ggataaacag gataaatcgt ttttcaaatc gagattcggg tttcattcag
     1201 agcttttgaa taaaaaagct tccatcctgt ttatcctatc tatcctgttt gaatcacttc
     1261 ccggaatctt cgggataata aatcaattcg acgatattgc cgtcgatgcc tttcgtgtaa
     1321 aaggaagaag ttccgtcgcg gtgcggtttt atcgggcgat tttcgcgcgc ggcggcttcc
     1381 ctgagctctt ccagagtttc gactttcatc ccgatatgcg gcggatgctt cgaattgggc
     1441 ggcagcagcg cgatgccgaa gcctttcgcg tcgcgcatca tcgcccaatc ttcgtaaacc
     1501 gtctcgaccg caaagccgag ccgcttgtat tcttcgaccg atttcgacaa atcctcgcac
     1561 ttgaccgcca ggtggtcaac cgttcccgca aaacgcaact cagacatttt atctcaaacc
     1621 tccgagatga ttttaccaaa gaaagtcgag agtccggagc ccggagtcga gaaaaatctt
     1681 tgactcgctg ctctggactc tcgactcgac aaccggattt acggtaaatt taactgttta
     1741 tgagaatcgc ttcgcttttg ccttcggcga cggaaatcgt ctgcgcgctc ggtctggaag
     1801 aaaatctaat cggcatcacg cacgagtgcg attatccgcc gcgggtcgcc ggaaagccgc
     1861 atttgaccgc cagccggatt tcgcacgaga cgatgagcag cgccgagatt gaccacgccg
     1921 tccgctcgca gctcgacggt cacggctcga tttacgattt aaacaccgcg ctgctggcag
     1981 agctgaaacc cgatttgatt atcacgcagg agctttgcga ggtctgcgcc gtcagttata
     2041 aaaccgtcga gcgagcggcg cgaatgtatg tcgccggcgc gcaggtcgtt tccctggagc
     2101 cgaacacgat tgaagatatt tttgccaata tcaaaaccgt cggcgaattg tgcaatgttt
     2161 cggaaaaagc ggaagaagtc gtaacgaatt tgcgggagag gctcgggcgg cttcgcgagc
     2221 aatcagccgc agccgaaacg cgcccgaaag tttttatgct cgaatggctg gagccgccgt
     2281 ttgcgcccgg tcattgggtt cccgaacaga cggaagcggc gggcggcatt tccctgctgg
     2341 gcaaagcggg cgaaaaatcg gtaatgacga cttacgaaaa aatcctcgaa accgcgcccg
     2401 atcgtctggt tctgattccg tgcggctatt acgcggcgga cattttgcgg cagcttccga
     2461 acacgatttt tcccgaaagt tttcgcgagc ttccggctgt aaaaacaggc gaggtctggg
     2521 ctttggacgc gaccgcttat ttttcgcgtc ccgcgcctcg cgtcatcgac ggcgcggaaa
     2581 ttctggcaaa gattgtgcat cctgaaatct tcggcgcgcc cgctgaaacg caagccgttc
     2641 gcgtttccaa aaatttgttg agattcagcc cggcggtttc aaattaatac gccgataaat
     2701 ttatacggaa gcgcgttgcg caaagcgaaa aacgggacgc ggatttgtta ggattgatta
     2761 agatttatta agataaaatc ctattttatc ctgatttatc ttattaatcg ggcgctccgc
     2821 ctgttttatt atgaaaattc ttatcgcact tttttacata gccgtttgtt cgctcgcctc
     2881 gctcgcccag acgaacgccg agattctcgc cagcgcgaac gggcagaaat tcaccgtcgc
     2941 cgacctgccg ccggatttgc gcgaggcgta taaacagcgc aatacgaaaa ccgctcagct
     3001 gagacaggac attttgacgc agatgattgt cgatacgctg ctggagagcg aagccgccgc
     3061 gcgcaaaacc gacgtcgaaa aactggaagc agaagccaga gccaaagccg ccgcgccgac
     3121 cgaagcgcaa atcaaagccg tctacaacgc gaacaaagca aaaatcggcg agcgaactct
     3181 ggaagagacg cgcgagcaga tcgtcgcttt tctgcgccgc gaaaacgaac aaaaggctct
     3241 cgtcgctttt gtcaaaagcc tgcaagccaa atacaaaatc gcgagcctca agcccttaaa
     3301 cgcgccgaat ctgaaagccg ccgacattct ggtcacggtc ggggctaaaa aaatcgccgc
     3361 cgggcagttc gagcaggttg ccaggcgtga gatttacgaa tatcgcgcgg aaatttacga
     3421 tgcggtcgtc aatacgctgg aagaagtgat ttatttaaag ctggtcgaag cggaagcgca
     3481 aaaatccggc attgcttcgg gcgatttgct ggcgcgcgaa atcaccgaca aaatgcgcga
     3541 tttttccaac gaagaaagcg agaaactgcg ctatgatttc cggcagagcc ttttcagaaa
     3601 atacaacgtg cagattctcg tcaaagagcc tgagcctttc gcgcaaaaca tttcgaccga
     3661 cggcgaccct tttcagggaa aggcgaacgc gcccgtgacg gtcgtgatgt tttcggattt
     3721 tcaatgctcg gtctgcgcgg cgacgcatcc gattttgcaa aaggttttag ccggatacgg
     3781 cgataaagtc cgttttgtcg tccgcgattt tccgctgacg ccgattcaca aaaacgcttt
     3841 tctcgccgcg caagccgcga atgccgccaa cgcgcagggc aaattttttg aatacaccga
     3901 gattctttat cgaaatcagg cggcgctcga cgccgattcg ctcaaaaaat acgccgccga
     3961 ttcgggttta aatgtcgggc aatttgagtt agacttgcaa agcggaaaat tcgccggcga
     4021 agtgcgccgc gatatggctg acggaaaacg ctacggcatc accggaacgc cgacgatttt
     4081 catcaatggc gtcaaggttc gcgtattatc ggcaaaatct ttccgcgacg cgatagaaaa
     4141 agcgctggcg aaaacaaaag tcagcgccgg ttcacgctaa ggaatttgcc acagggataa
     4201 atattttttc gaatcaggat ttataaaaat gcgtcttttc ggcttaatga tatttgcaat
     4261 ttttgccttt gcctgcggcg gcgaaacgcc accgataaat gccaattcta ccgtgaaaaa
     4321 taccgccgga aacaatagcc tcagcccgac cagtacggcg gcaaacaacg ccgccgcgcc
     4381 gccgaatgtt tcgacttacg aaatcgtcaa tacttttaga cacgattcga aagcttttac
     4441 gcagggcttg gttttccaaa acggtttttt ctatgaaagc accggcgaat acggggactc
     4501 gactttgcgc aaggtcgagc cgcaaagcgg aaaggtcttg caggagcacg agttagccag
     4561 agaatatttc gccgagggaa tgaccgtctt aaacggcaaa atttatcaaa tcacgtggca
     4621 ggaaaatacg gctttcgttt acgacctgga aaattttaaa ctcctgcgcg aaataaaata
     4681 tcagggttcg ggctggggct tgacgacgga cggcacgcac ctgattctga gcgacggcac
     4741 gcacatcata aagtttctcg acccggaa
//
LOCUS       DASWNE010000876         2365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_276981, whole genome shotgun sequence.
ACCESSION   DASWNE010000876 DASWNE010000000
VERSION     DASWNE010000876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276981"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..1897
                     /locus_tag="VGB68_17105"
     CDS             197..1897
                     /locus_tag="VGB68_17105"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0051015.1"
                     /translation="MTTTDFLTGTVKGPGELPHEYLFITKDNEKTRIGEFVYYRVKDG
                     EQTRQIIGTIKGRKIVRNLPDAFLADPSTPPALVSALIGLNGDACEIYEIQVETIGYF
                     DGNLKDFVNPRIPPNPGDAVFMTSSETLSEMLSPKRSTELGSAHIGSLLTRNAGEVPV
                     VLSIKDVVSTHLAILASTGSGKSYTAGVLVEELMNQYNRAAVLIVDPHGEYHTMQSIQ
                     GDDQFFGADGYKPEVKIFTPDKIKVRFSTLTEADVKYLLPEGTSDKMLHFLAQAFRSL
                     NERLRAERKQDYAYAYHDLRTEVMAQKYGDEKKAGDGGNVSSIDGLLWRLDSRFDRPD
                     SLFSAQDHIPLKDLFAPGRCTILELSDIEQHEQQVIVGTLLRRVNKARMLTTRGEAQA
                     NTENHLPYPVFTLLEEAHRFAPAGASVVSTNILKQILAEGRKFGVGIGLITQRPGKLD
                     QDVLSQCMTQIIMRIVNPIDQQTIAQSVEGAGRAMLAELPALTKGQAVISGVGINTPV
                     MCRIRKRLTVHGGETFDAPTEWMRWHSPDEQERREMDTSPYLKQSSDKKPEKIGNIRI
                     "
     gene            1959..>2365
                     /locus_tag="VGB68_17110"
     CDS             1959..>2365
                     /locus_tag="VGB68_17110"
                     /inference="COORDINATES: protein motif:HMM:NF016874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HEY0051016.1"
                     /translation="MADTKITTPKTFCFVLMPFSEEFDDVYRIGIKEACDKAGAYCER
                     VDEQIFSERILDRVYNQIAKADIVIADMTGRNPNVFYEVGYAHALGKLTILLTRNADD
                     IPFDLKHFTHIVYGTKISILRDELGKRVKFFIE"
BASE COUNT          635 a          626 c          558 g          546 t
ORIGIN      
        1 agaatcatcg ataaaacccc aacccccagc cagatttgcc gcagactcaa tctaaaactc
       61 tctttcattt ttgtgcctcc tgtaaagttt gctgctttct tagacaccgc ccgcaccttt
      121 ttgttcccga acaattaaaa ctttcgtgct atactttttc cgaattttcc aaaatcgaat
      181 ttccagtttt acaaatatga caaccacaga ttttctaacc ggcacggtca aaggtcctgg
      241 cgaattgccg cacgaatacc tttttatcac caaagacaac gaaaaaacgc gcatcggcga
      301 gtttgtatat tatcgcgtca aggacggcga gcagacgcgc cagattatcg gcacgatcaa
      361 aggaagaaaa atcgtccgca atctgccgga cgcattttta gccgacccga gcacgccgcc
      421 cgcgctggtt tccgcgctta tcggattaaa cggcgacgcc tgcgagattt acgagattca
      481 ggtcgagacc atcggctatt tcgacggaaa tttaaaggat ttcgtcaacc cgcgcattcc
      541 gccgaatccg ggcgacgcgg tttttatgac ctcgtcggag acgctctccg agatgctttc
      601 gccgaaacgc agcacggagc tcggttcggc gcatatcggc agccttctga ccagaaacgc
      661 gggcgaagtt cccgtcgttt tgtcaatcaa agacgtcgtt tcgactcatc tggcgattct
      721 cgcttcgacc ggttcgggaa aatcctacac ggctggcgtt ctggtcgaag aattgatgaa
      781 tcaatacaac cgcgccgccg ttttaatcgt cgacccgcac ggcgaatatc acacgatgca
      841 gtcgatacag ggcgacgacc agtttttcgg cgcggacggt tacaagccgg aagtcaaaat
      901 cttcacgccc gacaaaatca aggttcgctt ttcgactctg accgaagcgg acgtgaaata
      961 tcttctgccc gagggaactt cggataaaat gcttcatttt ctggcgcagg cgtttcgcag
     1021 cctgaacgaa agactgcggg cggaaagaaa gcaggattac gcctacgcct atcacgattt
     1081 gcgaaccgag gtaatggcgc aaaaatacgg cgacgagaaa aaagccggcg acggcggaaa
     1141 cgtttcctca atcgacggtc ttttatggcg gctcgattcg cgtttcgacc gtcccgacag
     1201 ccttttttcg gcgcaggacc acattccgct caaagacctt ttcgcgcccg gacgctgcac
     1261 gattctcgaa ctctcggaca tcgaacagca cgagcagcag gtcatcgtcg gaaccttgct
     1321 gcgccgcgtc aacaaagccc gtatgctgac cacgcgcggc gaagcgcaag ccaacacgga
     1381 aaatcatctg ccgtatccgg ttttcactct gctcgaagaa gcgcaccgct tcgcgcccgc
     1441 cggggcgagc gtcgtctcga ccaacatcct gaaacagatt ctcgccgaag gtcgcaaatt
     1501 cggcgtcggc atcggcttaa tcacgcagcg tccaggaaag ctcgaccagg acgttctctc
     1561 gcaatgtatg acgcaaatca ttatgcgcat cgtcaatccg atcgaccagc aaaccatcgc
     1621 ccaatcggtc gaaggcgccg gacgcgcgat gctcgccgaa ctgcccgccc tgaccaaagg
     1681 gcaagccgtc atctccggcg tcggcatcaa cacgcccgtt atgtgccgca tccgcaaaag
     1741 attaacagtg cacggcggcg aaaccttcga cgccccgacc gaatggatgc ggtggcattc
     1801 gccggacgaa caggaaaggc gcgaaatgga tacttcgccg tatctgaaac aaagctcgga
     1861 caaaaagccc gaaaagattg gaaatattag gatttgattc tcatgttaga ataaatttaa
     1921 ttgaaatagt tcgagcatac tcaagaattc aaattgttat ggctgacact aaaatcacga
     1981 cacctaaaac cttttgtttt gtcttaatgc ctttcagcga agagttcgat gatgtttacc
     2041 gcataggaat taaagaggct tgcgataagg cgggagcata ttgcgagcgt gttgacgaac
     2101 aaatatttag cgaaagaatt ctcgaccgcg tttataatca aatagctaaa gccgatatag
     2161 tgattgctga tatgacgggt cgtaatccta atgtttttta tgaggttgga tacgctcacg
     2221 ctttaggaaa attaacaata ttacttaccc ggaatgctga tgatattccc tttgacttaa
     2281 aacactttac gcacattgtt tatggaacaa aaatttcaat tcttcgagat gaattaggaa
     2341 aaagagttaa attttttatt gaaaa
//
LOCUS       DASWNE010000877        14480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_99994, whole genome shotgun sequence.
ACCESSION   DASWNE010000877 DASWNE010000000
VERSION     DASWNE010000877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 14480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14480
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99994"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..612)
                     /locus_tag="VGB68_17115"
     CDS             complement(76..612)
                     /locus_tag="VGB68_17115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051017.1"
                     /translation="MNAIRCPNCNSINQLSAVQCHQCQMPFGSLPQTAYVSVPTEQTF
                     EAQTLSFNPPPLSIPQDNEIGRKTFLWYRVYCGFLMVMYLLVTLLGVFLAAFAPDTRE
                     YKSEETFIMGIIYAVLGVVFFIVFAVALFLPRKPYNWIVGIVMMALGMTSCCFLPFLI
                     PLIIYWVKPETKAYFGRN"
     gene            complement(825..1730)
                     /locus_tag="VGB68_17120"
     CDS             complement(825..1730)
                     /locus_tag="VGB68_17120"
                     /inference="COORDINATES: protein motif:HMM:NF013395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEY0051018.1"
                     /translation="MKNKALTGGLIALAIIVLIIVLCFWVGKKGLDNLRGTDDTNITK
                     PDGQPQSPPAQPQTPEQAAQVYLALGNPSNANASDPNNYLLVNNYMAISYSRERAIPN
                     WVAWRVTRADMGDVDREDSFRPDDRLPKGWARATPADYTGSGFDRGHICPNADRNGSP
                     DSMAATFLMTNMTPQTPDLNRGPWQKLEAYLRTLVTRGSDVYIVSGVYGENGKIKKKV
                     TIPSNNWKIAVAVPAGSNLSAVNENTRVIAVDVPNVKGIKNADWQTYRTTVRDIERKT
                     GYNLLSNLPQNLQDALENRKDNINN"
     gene            1828..2910
                     /gene="rlmN"
                     /locus_tag="VGB68_17125"
     CDS             1828..2910
                     /gene="rlmN"
                     /locus_tag="VGB68_17125"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEY0051019.1"
                     /translation="MEKLHLTGLTQIELADFVVNQGEPKFRARQIFKGLHRRRALDFN
                     EITDLPKAFREKLGEIAAVSTLKVESRYVSEDGTRRFLMKTHEGLPVETVFIPTENRD
                     TICFSSQSGCPLKCDFCLTAKLGLLRNLTAGEIVEQIIIVLNDVYGAGAETPHGTNLV
                     AMGAGEPFLNFENLTRALHIMADEEGLFIVPNRVTVSTAGIVPKIYEFAKLRQRPHLA
                     ISLSAPNDELRDKLMPINKRWNLEELFAAAKEFEKSLRRGERFTFEYVLLGGVNDSDE
                     HARALANLLNKYGLQRVKINLIPHNSAEPLEYKPSSPEQVERFKAILESKGVSAYVRR
                     PRGRDIYAACGQLAAKTSPNLVQITA"
     gene            3011..3469
                     /locus_tag="VGB68_17130"
     CDS             3011..3469
                     /locus_tag="VGB68_17130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051020.1"
                     /translation="MEILILGIILVALMAYVSTRIKKSAAQAFEKETIETEDFRLVKP
                     EGFLSPASGNSGYAFEAYSKDFGEDDAAEYRKATVHIRKKTGRGEILPDETEKTEKGV
                     TIQTFRKILKTNKSGEIYELEISVLKEYREQFFDKIEETRNSFSLKQENL"
     gene            3466..3942
                     /locus_tag="VGB68_17135"
     CDS             3466..3942
                     /locus_tag="VGB68_17135"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HEY0051021.1"
                     /translation="MKNNAIKFILLAGLLFAIGAFSPEINAQSIDGSIGNGTVKRGGA
                     TRGTIVLNIPGGLHVNSNRPNNEYAIPTVVSISSREAKVSAVSYPRGASRKFGFSEKP
                     ISVYEGRATFGFNVTVPASFKGNVVRIRAVVKYQSCNDEVCFPPKTQEITLSARVQ"
     gene            complement(4087..4857)
                     /gene="pgeF"
                     /locus_tag="VGB68_17140"
     CDS             complement(4087..4857)
                     /gene="pgeF"
                     /locus_tag="VGB68_17140"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEY0051022.1"
                     /translation="MKILVCRALEEKGFVNGFSTRLGGVSKMPENDLNLSGFDDDSEE
                     NIMENRRRFLNVFDGDFTLATAWQVHGVDVKTVGNPDDAKDGNARFDALVSNLPGILV
                     GVKTADCVPVLLGDAKTKSFAAIHAGWRGTAQSIAAKAIEKMREAYDTKPEDLICAIG
                     PAAVCNYEIGQDVIDDFRKNFPDNSEHLFAPTRDGHAFVDLHRANFEQLAATGVLPEN
                     IYSAPFCTLERTDLFFSYRKEKRLYGKTGRLLSVIGRK"
     gene            4988..5368
                     /locus_tag="VGB68_17145"
     CDS             4988..5368
                     /locus_tag="VGB68_17145"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEY0051023.1"
                     /translation="MILSVGIDIIEVYRIRETIERTPRFVERVFTGGERAYCEAKGAA
                     AAQSYAARFAAKEACLKALKTGWRGRITWHDIEITNDENGVPSLQIRGAAREILESLG
                     ADSIHLSLSHTSEHAIATVILEKI"
     gene            complement(5809..6369)
                     /locus_tag="VGB68_17150"
     CDS             complement(5809..6369)
                     /locus_tag="VGB68_17150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEY0051024.1"
                     /translation="MRFEQRLSSRICGSRQNLARLLVEKNIRLVYGGGNIGLMGVIAD
                     EVMRGGGAVVGVIPDSLFKKEVGKREITELHVVGSMHERKAMMAELADGFIAMPGGIG
                     TFEEFFEILTWSQLGFHEKPCGLLNIANYYDGLLALCDHAVGEGFLRSVHRALILDDS
                     DAAALLEKMNNFQPPVIEKWIAKDDL"
     gene            complement(6390..6908)
                     /locus_tag="VGB68_17155"
     CDS             complement(6390..6908)
                     /locus_tag="VGB68_17155"
                     /inference="COORDINATES: protein motif:HMM:NF019932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0051025.1"
                     /translation="MKNFVIVLLFILTFSFTAFGQRTKAVVVKPDAKKEVQAAFERLT
                     EGIKQSDAKKVMSVYENSDRILFFNNNGTATIGWENMRTNRESLYAKTKNVTLDITGL
                     RVEMLGANSAYVSCKWKQTQEYNGKLESASGRMTLVFRLIGKEWKVIHLHTSPDNPDA
                     TRPVFPSERETQ"
     gene            complement(7160..7363)
                     /locus_tag="VGB68_17160"
     CDS             complement(7160..7363)
                     /locus_tag="VGB68_17160"
                     /inference="COORDINATES: protein motif:HMM:NF044816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0051026.1"
                     /translation="MEENLLSVAQVAEKRGVTIGRIHQLIQDGTLVAKKYGNQYLIKE
                     ADADALEIYGKAGRPPKEKIENK"
     gene            complement(7597..8280)
                     /locus_tag="VGB68_17165"
     CDS             complement(7597..8280)
                     /locus_tag="VGB68_17165"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEY0051027.1"
                     /translation="MCGRFTNRAKPEQIRDEFKVGKKNPDLYKERYNIAPAQLIDVVF
                     QPETERILSELKWGLVPSWAKDADIGNRMINARAETLTEKPSFREAFKKRRCIIPASG
                     FYEWQKKGSGAKQPFYFYLKEKEVFGFAGLWEEWIDKESGDVLETCTIITTEANDVLK
                     PVHERMPVILKAADYDQWLDPKENNTDRLKKLLAPYPAVEMSSHTVSKSVNIPDADSD
                     ELIKPLNSL"
     gene            complement(8326..8547)
                     /locus_tag="VGB68_17170"
     CDS             complement(8326..8547)
                     /locus_tag="VGB68_17170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051028.1"
                     /translation="MKKEYDVCEGFTIELLNKEVNEALSDGWECQGGVDVTHFVDVEN
                     KDVDFENLIFHQAIVREVEDEQNEDAQVK"
     gene            complement(8789..9970)
                     /locus_tag="VGB68_17175"
     CDS             complement(8789..9970)
                     /locus_tag="VGB68_17175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0051029.1"
                     /translation="MTTQNQVRENSGWSEERFRQLLESVKDYAIFITDLDGIIETWNK
                     GAEIIFGYTAEEAIGQLEAIIFTPEDRAGKIPEMEMKLARENSSAADERWHIRKDGSR
                     FYASGFQTALYENGKVTGYAKIARDLTERVRLEEQLHQTNTDLEARIQERTADLQNEI
                     IERKSSEEIRVKLLRRIVSTQEDERKRISRDLHDHIGQKLTALSLHLELVKQRCDDAE
                     LCRLIEHAQERAKEVDSELDFLAWELRPASIDELGLETALKNFANEFSRHFKVPVEFH
                     SRNFHNKRLLPEIEINLYRIAQEALNNIAKHAKASNVNVLLEKPDHHIVLIVEDNGIG
                     FDPREKANRNKGLGLVGMGERAALIGGSVEIESEDGKGTSIYARVPAKFVDEENTQTE
                     S"
     gene            complement(10057..10353)
                     /locus_tag="VGB68_17180"
     CDS             complement(10057..10353)
                     /locus_tag="VGB68_17180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051030.1"
                     /translation="MTDEDKFVSYDLIEAIKSFPIHSEIKHCGSTFSVSPFDIYTVCP
                     NCGTQIKLRSLSGAAELEDVFDAAFEWMAHPTAKEHFEKRQKEIIEDTDKDSVS"
     gene            complement(10995..13022)
                     /locus_tag="VGB68_17185"
     CDS             complement(10995..13022)
                     /locus_tag="VGB68_17185"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEY0051031.1"
                     /translation="MNARAATFTVTKTADTADGICNSDCSLREAIEAANAATSNDAII
                     FDATVFAVAQTIILNGGPTLQIDNSGTLIINGTGANLLTISGNNEYRVLVILNNANVT
                     INDLRIANGRLGGGGAGIRINGVLTLNNSIVSNNTGVTGAGGISNVGTTTINNSIISG
                     NSATSNGDGGGIQNGSGRLTINNSTISGNSSSFSGGGIHNSHGNITITNSTISGNTSV
                     GSGAGIYNTSGTISLMNTTISNNSALFNGGGIFVTGGSSSNLTNVTIAGNNASGRGGG
                     IYNSFTDTVLVRNTIIATNTGDAPDFNEGGISSQGYNLIGVGTTGVATDIIGQNPMIA
                     SLAANGGPTLTRALLPGSPAIDAADRNIFPGTDQRGAGRPRDGDNNGVTRADIGAYEF
                     DGVISNRTRFDFDGDGRADISVFRPAGRDWYIQNSATPTLYIRQFGANGDLIAPADFD
                     GDGKTDISVFRPSDGGWYRLNSSNNAFSALQFGSNGDLPVPGDFDGDAKADITVYRPS
                     AGSWYRINSSNNQFISAQFGIAEDKPLIGDFDGDGKSDLAVFRPSNGTWYRINSSNDT
                     FSPNQFGISEDKPVPADFDGDGKTDLAVYRPSVGDWYIINSSNSSFTGTHFGVTEDKP
                     APADFDGDGKADLAVFRPSTGTWYLLRTTAGFTGFQFGATADVPIPNAFVR"
     gene            complement(13251..13736)
                     /locus_tag="VGB68_17190"
     CDS             complement(13251..13736)
                     /locus_tag="VGB68_17190"
                     /inference="COORDINATES: protein motif:HMM:NF022461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2846 domain-containing protein"
                     /protein_id="HEY0051032.1"
                     /translation="MKDIRKDKKMRLFRSVQLLLAFVALIGFEQSIYAQSDDKSGASN
                     ADAVVYIYRYKQFIGYTVEPSVYCNEVPLARMDNGRYFKVVLSPGTYTFRSNDKQSGI
                     EMELKSGEQYYMRVEIVQGLWSQHGRLVLMAKEQGAYEIKKLKPLGQSKIKDRKRVVV
                     D"
     gene            14129..14227
                     /locus_tag="VGB68_17195"
     tRNA            14129..14227
                     /locus_tag="VGB68_17195"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14163..14165,aa:Sec,seq:tca)
BASE COUNT         3685 a         3452 c         3335 g         4008 t
ORIGIN      
        1 gtctgctgcg ccgcggcatt aaaggcgaaa accagaacga ataaaacaat caggattttt
       61 cttttcataa taaatttaat tacgtccgaa atacgctttg gtttcaggtt tgacccaata
      121 aataatcagc ggaatcaaaa aaggcagaaa acagcagctc gtcattccca gagccatcat
      181 cacaatgccg acgatccagt tataaggctt gcgcggcaga aaaagagcga cggcgaaaac
      241 gataaagaaa accacgccca gcaccgcgta aatgatgccc ataataaagg tttcttccga
      301 tttgtattcg cgcgtatccg gcgcaaaagc cgctaaaaat acgcccagaa gcgtgacgag
      361 taaatacatc accatcaaaa agccgcaata gacgcgatac cagagaaaag ttttgcgccc
      421 gatttcgttg tcctgcggga tggatagcgg cggcgggttg aagcttaaag tctgcgcttc
      481 aaaggtctgc tcagtcggga cggaaacata agcggtttgc ggcagggaac cgaaaggcat
      541 ctggcattga tgacactgga cagccgaaag ttgattaatc gaattacagt tcgggcagcg
      601 aattgcgttc attggtaaaa ctccatttat ttcggatttt ttatttgaaa attataaatc
      661 ggcgaagatt ttgcttttaa atgcgaaggc ggacgggtcg cgtgtcgcct cgaagtcgac
      721 attgatgatt ttaacttaaa gtttgattat ttgaaacaga tttgtcggcg cggaataata
      781 aaatctgcgc cgtttataaa ttaactttta aattacggct gaaattaatt gttaatgtta
      841 tcttttctgt tttccagcgc atcctgtaaa ttttgcggca gattcgacaa aaggttgtag
      901 ccggttttcc gctcgatgtc gcggacggtc gtgcgatacg tttgccagtc ggcgtttttg
      961 atgcctttca cgttcggcac atcgacggcg ataacgcgcg tgttttcgtt gacggcggag
     1021 agattcgaac cggcgggaac ggcgacggcg attttccagt tattgctcgg aatcgtcact
     1081 ttctttttaa tttttccgtt ttcgccgtaa actcccgaaa cgatgtagac gtcgctgccg
     1141 cgcgtcacga gagttctcaa ataagcttcc agcttctgcc acggtccgcg gttcaaatcc
     1201 ggcgtctgcg gcgtcatatt cgtcattaaa aacgttgccg ccatcgaatc ggggcttccg
     1261 tttcggtcgg cgttcggaca gatgtgcccg cggtcaaagc cgcttcccgt ataatcggcg
     1321 ggcgtggcgc gcgcccagcc tttcggcagg cggtcgtcgg ggcgaaaaga atcttcgcgg
     1381 tcgacgtcgc ccatatcggc gcgcgtcacg cgccacgcca cccagttcgg aatcgcgcgc
     1441 tcgcggctgt agctgattgc catataatta ttgacgagca aataattatt cgggtccgag
     1501 gcattggcat tgctcggatt tccgagcgcc agataaacct gcgcggcttg ttcgggcgtc
     1561 tgcggctgcg cgggcggcga ttgcggctgt ccatcgggtt tcgtaatatt cgtatcgtcc
     1621 gtgccgcgaa gattatccaa accttttttg ccaacccaaa agcacaaaac gataatcaaa
     1681 acgataattg ccagcgcgat caaaccgccg gtaagagctt tatttttcat aaattccgtg
     1741 tgcaggcgac atttacgaaa agtttaacat aaacgcgtga aattttcggt ggtgcgtttt
     1801 tggcgaaaat tagttaaatt aaattcaatg gaaaaacttc atctcacagg cttgacgcaa
     1861 attgagcttg ccgattttgt tgtaaatcag ggcgagccga agtttcgcgc ccgccagatt
     1921 tttaaaggtt tgcaccggcg gcgcgcgctg gatttcaatg aaatcacgga tttgccgaaa
     1981 gcgtttcgcg aaaagctcgg cgagattgcc gccgtttcga ctctgaaagt cgaatcgcgc
     2041 tacgtttccg aagacggcac gcgccgcttc ctgatgaaaa cgcacgaggg actcccggtc
     2101 gagaccgttt ttatcccgac cgaaaaccgc gacacgattt gtttttcgtc gcagtcgggc
     2161 tgtcctctga aatgcgattt ttgtttgacg gcaaagctcg gattgctcag aaacctgacg
     2221 gcgggcgaga ttgtcgagca gattattatc gttctgaacg acgtttacgg cgcgggcgcg
     2281 gagacgccgc acggcaccaa tttagtcgcg atgggcgcgg gcgagccgtt tctgaatttt
     2341 gaaaatctga cgcgggcgct gcacattatg gctgacgaag aaggtctttt catagtgccg
     2401 aatcgcgtga cggtctcgac cgccggaatc gtgccgaaga tttacgaatt tgccaagctc
     2461 cggcagcgac cgcatctggc tatttcgctg tctgcgccga atgacgagct gcgcgacaaa
     2521 ttaatgccga ttaataaacg ctggaatctg gaagagcttt tcgcggcggc gaaagaattt
     2581 gaaaaatctc tgcggcgcgg cgagcgattt acgtttgaat acgttctgct gggcggcgtc
     2641 aacgattccg acgaacacgc ccgagcgctg gctaacttgt tgaataaata cggcttgcag
     2701 cgtgtgaaaa tcaacctgat tccgcataat tccgccgaac cgctggaata taaaccttcg
     2761 agcccggaac aggtcgaaag atttaaagcg attctcgaat cgaaaggcgt ttcggcatac
     2821 gtgcgccgac cgcgcgggcg cgatatttac gcggcttgcg ggcagctggc ggcgaaaact
     2881 tcgccgaatc tggtgcagat tacggcttga aagtgaaaaa gggtaaaagt gaaaaagtta
     2941 gaaagtgaac gagtgaaaaa gtaatcgtta ttttccgacc gcaaacattt gcactttttc
     3001 actctatatt atggaaattc ttattttggg aattattctg gtcgcgctga tggcatatgt
     3061 ttcgaccaga ataaaaaaaa gcgcggcgca ggcttttgaa aaagaaacaa tcgaaaccga
     3121 agatttcagg ctcgtcaagc cggaaggttt tctgagtccg gcgagcggaa attccggata
     3181 tgcgtttgag gcttattcaa aagatttcgg cgaagacgac gcggcggaat accggaaagc
     3241 gaccgtccac attcgcaaaa aaaccgggcg cggcgagatt ttaccggacg aaaccgagaa
     3301 aaccgaaaag ggcgtcacga ttcagacgtt tcgtaagatt ttgaaaacga ataaatcagg
     3361 cgaaatttac gagcttgaaa tttcggtttt gaaagaatat cgcgagcaat tttttgataa
     3421 aatcgaggaa acgcggaaca gcttttcttt gaaacaagag aatttatgaa aaacaacgca
     3481 atcaagttta ttttattagc cggtctgctt tttgcaatcg gcgctttttc gcctgagatt
     3541 aacgcgcagt caatcgacgg ctcaatcggc aacggcacgg tcaagcgcgg cggcgcgacg
     3601 cgcggcacga ttgttttgaa tattccgggc gggctgcacg tcaattccaa ccgtccgaac
     3661 aacgaatacg cgattccgac ggtcgtcagc atcagctcgc gcgaagcaaa agtgagcgcc
     3721 gtctcatatc cgcgcggcgc aagtcgcaaa ttcggctttt ccgaaaaacc gattagcgtc
     3781 tatgaaggac gcgcgacctt cggttttaac gtcacggttc cggcaagctt taaaggcaat
     3841 gtcgtccgaa tccgcgccgt cgtcaaatat caatcctgca acgacgaagt ttgttttccg
     3901 ccgaaaacgc aggaaattac tttgagcgcg cgcgttcaat aaatcggatt caggattcag
     3961 gattcaggat ttaagattcg ggatttaaga ttcgggattt aagattcagg attcaggatt
     4021 gccggtagta aacgaccaat cctgaatcct gaatcctgaa tcctgaatcc tgaatcttaa
     4081 atccttttat tttcttccaa tcaccgaaag cagcctgccg gttttgccgt acagccgctt
     4141 ttcttttcga tacgagaaaa acaaatccgt tctttcgagc gtgcagaagg gcgcggagta
     4201 aatgttttcc ggcagaacgc cggtcgccgc gagctgttca aaattcgcgc ggtgtaaatc
     4261 gacaaaagcg tgaccgtcgc gcgtcggcgc aaaaagatgc tcgctgttat ccggaaaatt
     4321 tttcctgaaa tcgtcgatta cgtcctgtcc gatttcgtaa ttgcaaacgg ctgccggacc
     4381 gattgcgcaa attaaatctt ccggtttcgt atcgtaagct tcgcgcattt tttcaatggc
     4441 tttcgcggca atcgattgcg ccgttccgcg ccatccggcg tgaatcgcgg caaacgattt
     4501 tgtttttgcg tcgccgagca aaaccggaac gcagtccgcc gtttttacgc cgaccagaat
     4561 acccggcaga tttgaaacga gcgcgtcgaa tctcgcgttt ccgtctttcg cgtcgtccgg
     4621 atttccaacc gtttttacgt cgacgccgtg aacctgccac gccgtcgcca gcgtaaaatc
     4681 gccgtcgaaa acattcagaa aacggcggcg gttttccata atgttttctt cgctgtcgtc
     4741 atcgaaaccg gaaagattca aatcgttttc cggcatcttc gagacgccgc cgagccgcgt
     4801 cgaaaagccg ttaacaaaac ctttttcttc cagcgcgcgg caaactaaaa ttttcacgcc
     4861 gtttttttcg cgccagtaaa aacctttttc tgataaaatt tggtcttcag actcagtccc
     4921 gaagccggct gtttggtttt cggatttttc cggctgactg ctgattgctg actgctgacc
     4981 gctttctatg attctatccg ttggcatcga cataattgaa gtttaccgta tccgggaaac
     5041 tatcgaaaga acgccgcgtt ttgtcgagcg cgtttttacc ggcggcgagc gcgcttactg
     5101 cgaggcgaaa ggtgcggctg cggcgcagag ttacgccgcc cgctttgccg ccaaagaagc
     5161 gtgcctgaaa gccttgaaaa ccggctggcg cgggcgcatt acgtggcacg acatcgaaat
     5221 cacaaacgac gaaaacggcg ttccgagtct gcaaattcgc ggcgcggcgc gtgaaatcct
     5281 ggaatcttta ggcgcagatt ccatacatct ttcgctttcg catacgagcg aacacgcgat
     5341 tgcgacggtt attttggaaa agatttaaat tctttgggac gttttatttt agacaatatt
     5401 gccgtatccg tatttgctcc gagcagagct gttctcgaac aaatactcac aaaaaattcc
     5461 acgttgctgc cgtaagtata aacgagatat ttctttccga tttcaaatcc ataaccgcag
     5521 tcgccgccgc cgatgcctgt cgtgaccgat aaaatcgacg aattttttcc tttccagttt
     5581 ttatcgaccc tgaattttac cgttacaaat gatgagtttg gatttttgat tattctcagc
     5641 gcctcgccgg aaaaaatcgc attagaatct ttataagctt ttctgattgc ctgatttaga
     5701 gtcgtcttta caaacggagc actgcaggag caagccgttg tttcacttac gctcgcaaaa
     5761 accgtcagga cgcaaacaac ggaaaccaaa aaaagttttt tcataagttt acaaatcgtc
     5821 tttagcaatc catttttcga tgaccggcgg ctggaaatta ttcatttttt cgagcagcgc
     5881 agcggcgtcc gaatcatcca gaatcaacgc gcggtgaacg gagcgcagaa aaccttcgcc
     5941 gacggcgtgg tcgcaaagcg ccagcaagcc gtcgtaataa ttggctatgt tcagaagccc
     6001 gcacggcttt tcgtgaaagc ccaattgcga ccaggtcagg atttcaaaaa attcctcaaa
     6061 cgtgcctatt ccgccgggca tcgctataaa accgtcggca agctcagcca tcatcgcctt
     6121 gcgctcgtgc atcgagccga cgacatgaag ctccgtaatc tcgcgctttc cgacctcttt
     6181 tttaaaaagc gaatcgggaa ttaccccgac gaccgcgccg ccgccgcgca tcacttcgtc
     6241 ggcgataacg cccatcaagc cgatattgcc gccgccgtaa acaaggcgaa tatttttttc
     6301 gaccagaaga cgcgccaaat tttggcggct tccgcatatt cgggacgaaa gccgctgctc
     6361 gaaccgcaga aaacggtaat atttttcatt tactgggttt ctctttcaga cgggaaaaca
     6421 ggtcgcgtcg cgtccggatt gtcgggcgaa gtgtgcaggt gtatgacttt ccattccttg
     6481 ccgatcagac gaaagaccag agtcattctg ccggaagccg attcgagctt gccgttgtat
     6541 tcctgcgtct gtttccattt gcagcttaca taagcgctgt tggcgccgag catttcgacg
     6601 cgcaagccgg tgatgtcgag cgtgacgttt ttcgtcttgg cgtaaagcga ttcgcgattt
     6661 gtccgcatat tttcccagcc gatggtcgcc gttccgttgt tgttgaaaaa cagaatccgg
     6721 tcgctgtttt cgtacacgct catcactttt ttcgcgtccg attgcttgat gccttcggtc
     6781 aagcgttcaa aagccgcctg cacttctttt ttcgcgtcgg gtttgacgac gacagccttg
     6841 gtgcgctgtc cgaaggcggt aaaactgaaa gttaaaataa aaagtaaaac gatgacaaaa
     6901 tttttcattt tgatagtcct ttccagagaa acattttaca aagaaagtaa atgaataagc
     6961 tatttgttgt aatcacggat tttagccgga atgtaaactt tagagataca aataagaggc
     7021 aaaaacgtaa gcgatacaaa gcggacgaaa caaaacgtgc cttttcacac gaaatttgtt
     7081 aaactgccct tgcattagaa aacagcttat tcagttttca aaacaccgaa gcctttgatt
     7141 ttacgagaat cagaggcttt tatttatttt caatcttttc tttcggcggt ctgccagcct
     7201 tgccgtagat ttccagcgcg tccgcgtccg cctctttaat caaatactgg ttgccgtatt
     7261 ttttcgcaac gagcgttcca tcttgaataa gttgatgtat tcgcccgatg gtcacgccgc
     7321 gtttttccgc cacctgcgct acagataaaa gattttcttc cataacaaaa attattctaa
     7381 attattttca aaaaaactgc aaactcttct agttttaatc gcgctattct gtaagagttt
     7441 atagaataat tgcaagttaa gcggttttaa aaaacaaatc aaaaagaaat caatcaaatg
     7501 tttattttaa aaggaatcga acagcttcaa aacgcaatcg acaaagcgca ctggcataag
     7561 cagccacact cttttattcc gcttcgcaag gtaaatttag agactgttta aaggtttaat
     7621 taactcgtcc gaatcggcat cgggaatatt cacgcttttg cttacagtgt gtgagctcat
     7681 ttcaacagca ggataaggag cgagcagttt tttcaggcgg tcggtattat tttctttcgg
     7741 gtcgagccat tggtcataat cagcggcttt aagaatcacc ggcatccgct cgtgaacggg
     7801 tttcagaaca tcgttcgctt cggtcgtaat gatggtgcag gtttccagca catcgcccga
     7861 ttccttatca atccattctt cccagagtcc ggcgaatccg aaaacttcct tttccttgag
     7921 ataaaagtaa aacggctgct ttgcgcccga gcccttcttc tgccattcgt agaaaccgga
     7981 ggcgggaatg atgcaccggc gctttttaaa ggcttcgcgg aatgacggct tttccgtcag
     8041 agtctcggcg cgggcattta tcattctatt tccgatgtcg gcgtctttcg cccacgatgg
     8101 aaccaagccc catttcaact cggaaagtat tctttcggtt tcaggctgaa aaacgacatc
     8161 aatcagctga gccggcgcga tgttatatcg ctctttgtaa aggtcgggat tcttttttcc
     8221 gactttaaat tcatctcgta tttgttcggg ttttgcccgg ttcgtgaatc ttccgcacat
     8281 acttttattt cctgcatttt ttaccggata tgggaaattt tcatattatt taacctgtgc
     8341 gtcttcgttc tgctcgtcct ctacttctct gacgattgcc tgatgaaaaa taagattttc
     8401 aaaatcaaca tccttatttt cgacatcgac aaagtgcgtc acatctacgc cgccctgaca
     8461 ttcccagccg tcacttagtg cctcattaac ctctttgttt aacaattcaa tagtgaagcc
     8521 ttcacaaaca tcgtattctt ttttcattat caattctccc gttgccttgt ttaaattact
     8581 tataaataca atcatagcaa gtttacacgc aatattttat ctcattaaat aacggagccg
     8641 gaattattgt tctggagcgc gtcaaaaacg tttcaaaagc catacgtttt cgagatttta
     8701 gtcgtgagac ggtttatgtg ccgaaactga aaaattcgga aaccgggttt tgagccttaa
     8761 tcagctttca ataagcattt cagaatattt acgattcagt ttgcgtattt tcttcatcga
     8821 caaatttagc gggaacacgg gcataaatac ttgttccttt gccgtcctcc gattctattt
     8881 ccaccgaccc gccaatcagc gccgctcttt ctcccattcc aaccagaccg agccctttat
     8941 ttctgtttgc cttttctctt gggtcaaatc cgattccgtt gtcttcgaca atcaaaacaa
     9001 tatgatggtc gggcttttcc aaaaggacgt ttacattact ggctttggcg tgtttggcaa
     9061 tgttattaag tgcttcctga gcaatgcggt agagattgat ttctatttcg ggaagcaggc
     9121 gtttattgtg aaaattacgc gaatgaaatt cgacgggcac tttgaaatga cgcgaaaatt
     9181 cattggcgaa gttcttaaga gcggtttcca gtcctaattc gtcaatcgaa gcaggtctga
     9241 gttcccaggc gagaaaatcc agttccgaat cgacttcctt agccctttcc tgcgcgtgtt
     9301 cgataagccg gcataattcg gcatcatcgc atctttgttt gaccagctca agatgtagtg
     9361 aaagagccgt taatttctgt ccgatatggt cgtgtaaatc acgcgatatt ctttttcttt
     9421 cgtcttcctg agtgctgacg attctgcgca gaagttttac acgtatttcc tccgagcttt
     9481 ttcgctcgat aatttcattc tgcaaatcag ccgttctttc ctgaatcctc gcctctaaat
     9541 cagtgttcgt ttggtgaagc tgttcttcaa gcctgactcg ttcggttaaa tctctggcga
     9601 tttttgcata accggttact ttgccgtttt catagagggc agtttgaaat ccgctggcat
     9661 aaaaccgtga gccgtccttg cgaatgtgcc agcgttcatc cgccgcgctg ctattttcgc
     9721 gcgctagttt catttccatt tcgggaatct tcccggctct gtcttcgggc gtaaatataa
     9781 tcgcttcaag ctgaccgatg gcttcttctg ccgtatagcc gaatattatt tccgcgccct
     9841 tgttccacgt ttcgatgata ccgtccaaat cggtgataaa aatggcgtaa tctttaaccg
     9901 attccagcaa ttggcgaaag cgctcttcgc tccagcctga gttttcccgg acctggtttt
     9961 gtgtagtcat agctttgttt tgcagaaaaa tcatatcaaa aatcgatgtt tcacaataac
    10021 gaaaaaccag taggagccgg gctcagaaag gctcgtttac gacacagaat ccttatctgt
    10081 atcttcaatg atttctttct gtcgtttttc aaaatgttct ttagctgtcg gatgagccat
    10141 ccattcaaac gcggcatcaa aaacatcttc caattcggct gctccgctaa gagaacgtag
    10201 tttaatctga gtcccgcaat ttggacaaac agtataaata tcaaatggcg aaacagaaaa
    10261 agtcgaacca caatgcttga tttcgctatg aataggaaaa gactttattg cctcaatcaa
    10321 atcataagaa acaaatttat cttcatctgt catatcttaa aatgaatttc ctgaataagt
    10381 ctcaaaaagg gtcgtttatg agacggcaga atcatttttc agggtttgtc gcgccccaaa
    10441 taatcagacc gagaaataac gtaacgacac tagtgaaaaa gtattcccac tctttgtcat
    10501 tatcggtgaa cagactccac agcaatcgta aaaactttag aatcgaaagt acaaaaaaca
    10561 ttccataaaa tgaacgtgcc gtattgcttc cgaatatttt cataaatggt ttttatgtgc
    10621 ctcaaaaagg ctcgtttacg gcgcggcatc gacgtatccg catccacaac actcttgtaa
    10681 tactcgaagc ggaaaaaaat agtaatagag cgggacgctg tttagtccga tagcaacggc
    10741 agccagaaag atattcgcgt tgagcaggag tcgctccata tattgaatat attggaattg
    10801 aattgtgaaa accagctttt tcttaatcac ttcaaactct gaacaaattt acgacaaata
    10861 agtctgaaaa aggcttgttt ctaaaacagg tattatcaca ctctttccat ttcgtttttg
    10921 agataatcgg acttgcaaaa aaggcttcac tcgaaattga acgaagccct ttttactttc
    10981 aatagaactg gattttacct gacgaaagca ttaggtatcg gcacgtctgc ggttgctcca
    11041 aactgaaagc ctgtaaagcc agccgttgtt cggagcagat accaggttcc cgtcgatggg
    11101 cgaaaaacag ccaaatcagc ttttccatct ccatcgaaat ccgccggcgc cggtttatct
    11161 tccgtcacac cgaaatgggt tccggtaaac gatgagttac tgctgttgat aatataccaa
    11221 tctccgaccg acggacggta aaccgccaaa tcagtcttgc cgtcgccgtc gaaatcagcc
    11281 gggacaggct tgtcttcact gattccaaac tgattcggcg aaaacgtatc gttgctgctg
    11341 ttgatacgat accacgtccc gtttgacgga cgaaaaacgg ctaaatcact ttttccgtcg
    11401 ccgtcgaaat ctccgattaa aggcttgtct tcggcgatac cgaattgggc ggaaatgaat
    11461 tgattgttgg aactgttgat tcgataccag cttcccgctg acggtcgata aaccgtgatg
    11521 tcggctttcg cgtcgccgtc gaaatctccg ggcacgggta aatctccatt gctgccgaat
    11581 tgcaacgccg agaaagcatt gtttgatgag ttcaagcgat accagccgcc gtccgacggt
    11641 cgaaaaacag aaatatcggt tttgccgtcg ccgtcaaagt cggctggcgc gattaaatcg
    11701 ccgttcgcgc cgaactggcg aatgtaaagt gttggagtcg cgctgttctg gatataccaa
    11761 tccctgccag ccgggcgaaa taccgagatg tcggctctcc cgtcgccgtc aaaatcgaat
    11821 cgtgtgcggt tagaaataac gccgtcgaac tcgtatgcgc cgatgtcagc ccgggtaacg
    11881 ccattattat ctccgtcccg tggtcgccct gccccgcgct ggtctgttcc gggaaaaata
    11941 ttacggtcgg cggcgtcaat ggcggggctg ccggggagta aagcccgagt tagcgtcgga
    12001 cctccatttg ccgctagaga ggctatcatc ggattttgtc cgattatgtc ggtcgccaca
    12061 cccgtcgttc cgacgcctat caggttatag ccctgcgagc tgattcctcc ctcgttgaaa
    12121 tcgggcgcgt ctcccgtatt ggtcgcaatg atggtattgc gcaccagcac cgtatccgta
    12181 aaactattat aaatgccgcc gccgcgcccg ctggcgttgt tgccggcaat cgtcacgttt
    12241 gtcaggttgg aggagctgcc gccggtgacg aaaattccgc cgccgttaaa taacgcggaa
    12301 ttgttgctga tagtggtatt catcaaactt atggttccgc tcgtattgta aatgcctgcg
    12361 ccggagccaa cgcttgtgtt tccgctgatg gttgagttgg taatcgttat attaccgtga
    12421 gagttatgga tgccgccgcc gctgaaggac gaggaatttc cgctaatcgt cgagttgtta
    12481 atggtcagtc gcccgcttcc gttttgaatt ccgccgccat cgccgttact ggtggcggag
    12541 ttaccgctga taatcgaatt gttaatggta gtcgttccga cattgctgat gccgccggct
    12601 ccggtgacgc cggtattgtt gctgacgatt gagttgttga gcgtgagcac tccgttgatg
    12661 cgaatgccgg cgccgccgcc gccgagcctg ccgttagcaa ttcttaaatc gttaatcgtc
    12721 acatttgcat tattcaagat aaccaaaacc cggtattcgt tgttgccgct aatcgttaat
    12781 aagttggctc cggttccgtt aataatgaga gtgccgctgt tatcgatttg cagtgtcggt
    12841 cctccattca ggatgatggt ttgcgcgacg gcaaacacgg tcgcgtcaaa aatgatcgcg
    12901 tcattagagg tcgccgcatt tgccgcttct attgcttcgc gcagggaaca atccgagttg
    12961 cagataccgt cggctgtgtc tgcggttttt gtgacggtaa aggtcgctgc ccgggcattc
    13021 aatattaata atattaacac tagaaatatt aacgatattg aattcctggt aattttcgtt
    13081 ttttcgatgg ataaaaaaac tgtcaaaaag gtgttttgtt tcataatcat ttaactgctt
    13141 gttctgttga agaataattt tttggagtac agtcgtttct gactgccggt attcgtccga
    13201 aaagggtcgt tttcgagatt tgatatctat tattgttttt gctgttgctg ttaatcaact
    13261 acaacccttt tacgatcttt aattttactt tgaccgagcg gctttagctt tttgatttca
    13321 taggctccct gctcctttgc catcagtacg agtctgccgt gttgactcca taaaccctgc
    13381 actatttcta ctctcatata atattgttcg ccgcttttca attccatctc gatacctgac
    13441 tgcttatcat ttgagcgaaa agtgtaggtg ccgggactga gcacgacttt gaaataacgt
    13501 ccattatcca ttcgcgccag aggcacttca ttacaataaa cggacggctc gaccgtataa
    13561 ccaataaact gtttgtaacg gtagatataa actacggcat cggcgtttga cgcgcccgat
    13621 ttgtcatcag actgtgcata aatcgattgc tcaaaaccga ttaaagctac aaaagccagc
    13681 aataattgaa cgctcctaaa caaacgcatc tttttgtcct ttctaatatc tttcatatct
    13741 tttacctgag tgcctcaaaa aagggcgttt ttgagccgga attattctaa taaaaatata
    13801 tctttcagtc agttgccgca tagctttcgg caaagatgag atttacagtc attttaacat
    13861 tctttacgcg cctgtcttta attgaaacaa cagttttcgg tgttccataa aaggcttcgg
    13921 attcttctat tgaaaaaagc tcccgattag attcaacagc agtcattata ctccaaacaa
    13981 atagaccgta gttaccaaag acctcggttg attctctttt ataagtcttt ctcgcggcga
    14041 tttaatattt tatgcgcgtt gaaaagataa gaaacttgac agtcgttcgc acaaaagttt
    14101 agttttaatt cctgactcgt ctaaatgcgg aggcgcatct gttccggtga atggcgcggt
    14161 cttcaaaacc gtttgtgaga ctgtgagagc aggctcaggt cggttcgact ccgacacgcc
    14221 tccgccaaaa taatttcgga tttcagattt tccattggcg aatggataag gaagaaatga
    14281 aacacggatt ttttcggatt gatatagatt gggattttta tttaaatgaa tactgaataa
    14341 ttcttatcaa tcttgttaaa tcggcatcgg cttctttatc gaaatagaaa atctgaaatc
    14401 ccaagcccga accaatatga aagaatggat ttctctgaat atgactccat tcgtcggacc
    14461 tcgcgcggcg acgaagctgc
//
LOCUS       DASWNE010000878         1675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_665541, whole genome shotgun sequence.
ACCESSION   DASWNE010000878 DASWNE010000000
VERSION     DASWNE010000878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665541"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(744..1559)
                     /locus_tag="VGB68_17200"
     CDS             complement(744..1559)
                     /locus_tag="VGB68_17200"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY0051033.1"
                     /translation="MLTTFLCFLTEVYAAPGDLDTTFSLDGKSFEPIGTGGSDIIRGT
                     ALQSDGKAVVAGYSFTGSNYDFSLVRYNTDGSLDTAFDSDGKVTTAVSGSTEEINALG
                     IQADGKIVAAGYANSGAGANFAVSRYETTGALDTSTYGTSGNVNFDVRGGSSDVIYGA
                     ALNSIGRAVVVGEASGLTTVARILGDTAPRLVNFSVSGRVQSGAGGISGVIVTLVDSN
                     GETHRTRTNAFGYYQFSEVSPGTSVITVSAKRFVFAVPTRIVDIGDVFFTAIE"
BASE COUNT          448 a          407 c          411 g          409 t
ORIGIN      
        1 ctgcaaatca gcttccgcca catacagcga aagtctgtaa acgtccggcg cgatttccgt
       61 gattttcata attttctcct tctaaagttt taaagcgaaa aatcgctttt caaagacatc
      121 acgtttgagg aaatcgaaag gttacaacgc tggaatttta tttttgctga ttagcttttc
      181 ctacctatcg acaacacttt ctctaaaaaa tcacaaatga ggatggatat gactttttat
      241 tattactaat aacaaagaat gattgaaata aatcaaaaga agaaattttc attttatgcg
      301 ctgaaaatgg cggaaatttt cactttatga gcgaatattt tttcatttta cgcgcgaatt
      361 atttttctaa gttgttgatt ttgcgggaag ccgattttca atttatggaa aatccgacgg
      421 gcgcgcgcta attgacagat aaagagaaat tcttatagcg taaagtgaaa acggcgggtt
      481 ttgtaatgaa agtatcagat agctgacaat tcagacagaa aggcataaaa ccagtctatg
      541 cctttctact gtcgggctct tgagagaatc gttccggagc gatgccgaaa cggctttcgc
      601 tcctttgcgc cggacgagcg cggcgagact gtcaggcgcg catccaaagc attaaacacg
      661 gtttctttga taaattgaat acccggcagc atatccggat attcctatcg gaacggctat
      721 gtttgagata gcttccggtt cgattattca atcgccgtga aaaatacgtc gccgatgtcg
      781 acgatgcgcg tcggaactgc aaaaacaaag cgcttggcag agacggtgat gacgctcgtg
      841 ccgggcgaaa cttccgagaa ctggtaatag ccaaacgcat ttgttcgcgt cctgtgtgtt
      901 tcgccgttcg aatcgaccag cgtgacgatg acgccggaaa tgcctccggc tcccgattga
      961 acgcgtccgc tgaccgagaa attaacgagg cgcggcgcgg tgtcgccgag aattcgggca
     1021 acggtcgtca agccggaagc ttcgccgacg acgacggcgc gaccgatgga attgagcgcc
     1081 gcgccgtaaa tcacgtcgct gcttccgccg cgcacgtcga agtttacatt gccgctcgtt
     1141 ccgtaagtag aagtgtcgag cgcgcccgtt gtctcgtagc ggctgaccgc gaaattagcg
     1201 cccgcgccgc tgttggcgta accggcggcg acgattttgc cgtccgcctg aatgcccaga
     1261 gcgttgattt cttcggtcga gccggaaaca gccgtcgtca ctttgccgtc cgagtcgaaa
     1321 gcggtatcga gcgagccgtc cgtattgtag cgaaccagag aaaaatcata attcgagccg
     1381 gtaaaactat aaccggcgac gaccgctttg ccgtccgatt gcagggctgt tccgcgaata
     1441 atatcgctgc cgcccgtgcc gatcggctca aacgatttgc cgtcgagcga aaaggtcgta
     1501 tccaaatcgc cgggcgcggc gtaaacctct gttaaaaaac acaaaaacgt ggttaacaaa
     1561 agacacgctg caaaagctgt ggacttcgcg aacaacaagg taataaataa gccataaaat
     1621 ccttgtaaat ccgcgccctt tcaccttcaa tcagacttcc cagatgattt cgatg
//
LOCUS       DASWNE010000879        10208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_380765, whole genome shotgun sequence.
ACCESSION   DASWNE010000879 DASWNE010000000
VERSION     DASWNE010000879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10208
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380765"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1592)
                     /locus_tag="VGB68_17205"
     CDS             complement(<1..1592)
                     /locus_tag="VGB68_17205"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HEY0051034.1"
                     /translation="MIRAKNRKSLLLFSKRIVIFLAAVLIFAPAGGNARAQLFEDFDD
                     VTLLPGRGWFKQNNSNPVGLNWFQGNHNVFPAQSGTPTSYIGANYGSTTGENTISNWL
                     LTPNLTLYNGGVIKFWTRTTSNVTFPDRLELRLSTNGASTNVGTTPTSVGDFTRLLLT
                     INPDLTEDGYPTVWTQYTISLSGLPPQGVSGRVAFRYFVTDGGTMGNNSFYIGVDTFS
                     YLPIIGDPFPRAPLDYNGDGKTDFAVVRQTGGGLGGQVTWFIHNGTTHTQTAWGSTTD
                     FFVAGDFDGDGKTDITVYRPQGGSSFFYTLRSSNGTFTARQLGTAIDDPTVVGDYDGD
                     GIADYAVYRDGGQPGQKSFWYYLGSATPGGAIRYVEWGQNGDAPIPGDFDGDGKNDFC
                     VQRPDGGGLGVFHLNKSGGGLETVYWGRASDSVLPGDYDGDGKDDFAVTRGSGSGHIA
                     WNVLGRNNNNIIHSGLLWGVNGNDFPAPGDYDGDGKNDIAVWSPNANSQQTYFFVRQS
                     SNAALRTFEWGQQGDYPVANYN"
     gene            complement(1802..3889)
                     /locus_tag="VGB68_17210"
     CDS             complement(1802..3889)
                     /locus_tag="VGB68_17210"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEY0051035.1"
                     /translation="MQKRFFPSLVLASAVILLSVFSVLAQQVQRAPFDVSNYRMEVQL
                     NPSENQLQAVADVTFTPLAETRTVAFELNGSLKVESISRVGSAAATITPATPKGKTTA
                     PSTASQSQITFVQDQVGVSDLGPSVKVDLGDLIPANVPVTLRFKYSGVLATPEGGPLL
                     TKRLAYVGDKNGYLMYAARWFPFHNYAADRATSDITITLPSGYQIIGYSDAPVSATGG
                     KYRFTQSKAALPGNFAYGRYTPKTLRFADYELQFYTKAGSDALISTYGETLGRALDFY
                     TKQYGEPDSGKRLIIVQTDDETLDFYSAPGMIFIADKFFDQSRQIAAERLQREAAYQW
                     WGQTVGLKSFDDAWLSQGLAEWSALELREAGISNTKLEALQRETLEKALTFEQTASLL
                     RAPSALDDTSTAYQYIMYGKGALVYRLLKETLGAQKFSQLLRQFLAQYRGKNASIDDF
                     EKLTTQVAGQPMRYFFARWVEGTGVPEFAVEYQIIRTREGKFVTRGTIKQNYDNLRLP
                     VDVQLVAEGEGELKTQTVLIEDASADFNIESNGKPLEVVIDPKYKLLRISDDLKVSSV
                     ARRGIEQFKEGNLAEAQQQFEAALKLDRNNSWIYYNLGNLFLEQRNYDLAIDNYKATL
                     SGNLRPNWLEVWANIKMGNAYDAKGDRTRAVAAYTRAQASGDDYDNAQEAVKRYMATP
                     YDPRQKTTTTAVK"
     gene            complement(4027..4500)
                     /locus_tag="VGB68_17215"
     CDS             complement(4027..4500)
                     /locus_tag="VGB68_17215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051036.1"
                     /translation="MLEVHIEVDDELAARGYRKALRAKAVNDIVYFYASKLMVVWLVV
                     AFVDAYAGVARLIPLHLLFLSGLGVAASVYSYLDWEKRVSQMKGWSFRARLDEMGVTT
                     TAPQENWYEWSSYKGYREFDDYLEIERLPGEISFLPKTPDLFQAIEFTKEKIPPK"
     gene            complement(4505..5308)
                     /locus_tag="VGB68_17220"
     CDS             complement(4505..5308)
                     /locus_tag="VGB68_17220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HEY0051037.1"
                     /translation="MELAVEKYKVESEPYYLPVAQEVELFQAAYAAKLPVMLKGPTGC
                     GKTRFVEHMAWRLGRPLITVACHEDLSATDLVGRFLLEGDETVWHDGPLTKAVRAGAI
                     CYLDEVVEARKDTVVIIHPLTDDRRRLPIEKRGTVVDAPPEFMLVVSYNPGYQSILKD
                     LKQSTRQRFVAMEFDYPPAESETEIVAREGGVDEATARDLVTIGQKVRNLRGHGLEEG
                     VSTRLLIYAAQMISKGIAPVNAAEVALCSPITDDRELQRSIREIVTTVI"
     gene            complement(5563..6345)
                     /locus_tag="VGB68_17225"
     CDS             complement(5563..6345)
                     /locus_tag="VGB68_17225"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY0051038.1"
                     /translation="MTNYETILVEKRESVAIITINRPEKLNALGRKVHEEGVAALDEL
                     RRDAAVCVVVVTGAGEKSFVAGADIAEFAGETPASQRNLFYERTLFNSIDTFPKPVIA
                     MINGFCLGGGCELALACDIRVCSENARFAQPEINLGIMCGGGGTQRLTRLIGEGRAME
                     MILTGDMIDAAKALQFGLVNYVWTREELETKTLELAGKIAQKAPIALQLSKEAVKLAS
                     RSNLDEGLRREVDLFAICFSTEDKEEGVAAFLEKRKPAFKGK"
     gene            complement(6431..6808)
                     /locus_tag="VGB68_17230"
     CDS             complement(6431..6808)
                     /locus_tag="VGB68_17230"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY0051039.1"
                     /translation="MSTNLQPQYEEKRFALRMALRLPIVVSGRADDGAAWSEPTETDD
                     ISTTGALFHLNQKVAQGENLYIRSHRPDGVPVEVQARVVRVSPSNYGTARIGVAITET
                     KENWLRLFVAWIADDQPSDEEEK"
     gene            7057..8064
                     /gene="trxB"
                     /locus_tag="VGB68_17235"
     CDS             7057..8064
                     /gene="trxB"
                     /locus_tag="VGB68_17235"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011178815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEY0051040.1"
                     /translation="MPETEINRKVVILGSGPAGLTAAIYASRAQLQPMVIDGPQPGGQ
                     LTITTDVENYPGFAKGIMGPELMNEFRAQAERFGTEFLNVWIDKVDLSQRPFKLYGKA
                     SPDSAEISTIVKAETLIISTGASAKWLGVPGEEPAPAGLGGNGVSACATCDGFFFRGK
                     EVVVAGGGDTAMEEATFLTRFASKVTIVHRRDSFRASRIMQERALANEKINVLWNTEI
                     REIQGTKEAGVTGIKIYNNQSKEETVFPTEGVFIAIGHKPNTDLFKDWLELNEVGYIQ
                     TEGRTMKTNIEGVFACGDAQDHYYRQAVTAAGTGCMAAIDAERFLAEHGEAERVTATN
                     W"
     gene            complement(8167..8736)
                     /locus_tag="VGB68_17240"
     CDS             complement(8167..8736)
                     /locus_tag="VGB68_17240"
                     /inference="COORDINATES: protein motif:HMM:NF019819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HEY0051041.1"
                     /translation="MRKWILAICALTLVSAATCVQAQNTKTGELKTVSFVDVKRYGGT
                     WYEIARYANKAQKQCVGNTTATYNFRAGGEVEVVNKCLTKDGKTEDATGMAKIIDTQT
                     NAKLKGSFAWLSSAPYWVIDLDENYQYAVVGNPDRKYLWILSRTPEINETTYQNILRK
                     VEIAGYNPAKLEKTPQKLETVKGAVVKKQ"
     gene            8980..9312
                     /locus_tag="VGB68_17245"
     CDS             8980..9312
                     /locus_tag="VGB68_17245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEY0051042.1"
                     /translation="MPIYEYRCADCAAHLETMQKISDAPLTVCEACGGKLEKQISRSG
                     FQFKGSGWYVTDYSNKGKSDTKTEAKSETEAKSETKTEKSSAGESTAGGDAASKPADS
                     SPKTSKTE"
     gene            9319..>10208
                     /locus_tag="VGB68_17250"
     CDS             9319..>10208
                     /locus_tag="VGB68_17250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0051043.1"
                     /translation="MKFSAKKALFLSLCVLLISVSALAQSRRSAKDTSGDGGTILSVV
                     AARANGSTEPIKAENLFLYENGIEQKIKNFTFDPSPSRIVVLVDNSQTLRAEVETLKN
                     AVKEFVYEIYEGDQVFVVAYDETPEIIQEWTDDAKKVETSLNTFRKQGNPYLFDALNA
                     AAREILLPLMPGTRKTAVVLISDGLDRGSKTAFDKILGELQSDNITVYALQLPDRTNG
                     AYRRNQPKAAQVITELTEGTGGKIFDFEQPQVAAKAICDELRKNRYLLSYFSLNASSF
                     DARRVFLLGQEGISVRTKNA"
BASE COUNT         2372 a         2787 c         2612 g         2437 t
ORIGIN      
        1 ctgttgtagt ttgccaccgg ataatcgccc tgctgtcccc attcaaaggt gcgcagagcg
       61 gcgtttgacg actgcctgac gaagaagtaa gtctgctgcg agttggcgtt cggactccag
      121 acggcgatat cgtttttccc gtcgccgtcg taatcgcccg gagccggaaa atcgttcccg
      181 ttcactcccc acaacagtcc cgaatggata atgttgttat tgtttcgtcc cagtacgttc
      241 caggcgatgt gtccgctgcc gctgccgcgc gtcaccgcaa agtcgtcctt gccgtcgccg
      301 tcataatcgc ccggcaagac tgaatcgctc gccctgcccc agtagaccgt ttccagcccg
      361 ccgcccgact tatttaaatg aaagacgccc agcccgccgc cgtcggggcg ctgcacgcag
      421 aagtcgtttt tgccgtcgcc gtcgaaatcg cccggtatcg gagcatcgcc gttctgtccc
      481 cattcgacgt atctaatcgc gccgccgggc gtcgcgctgc ccagataata ccagaagctc
      541 ttttgccccg gctgcccgcc gtcgcgataa acggcgtaat cggcaatgcc gtcgccgtcg
      601 taatcgccga ctacggtcgg gtcgtcaatc gccgttccca actgacgcgc ggtaaatgtg
      661 ccgttcgaac tgcgcaaggt gtagaaaaac gagcttccgc cctgcgggcg gtaaacggtg
      721 atatccgtct tgccgtcgcc gtcgaaatcg ccggcaacga aaaaatcggt cgtgctgccc
      781 caggcggttt gggtatgtgt cgtcccgtta tggataaacc acgtcacctg accgcccaaa
      841 ccgccgccgg tctggcgcac gaccgcgaaa tcggttttgc cgtcgccgtt gtaatcgagc
      901 ggcgcgcgcg ggaaaggatc gcctatgatc ggtaggtaac taaatgtatc aactccgatg
      961 taaaaggagt tgttgcccat cgttccgcca tcggtgacga aataacgaaa agcgacgcga
     1021 ccgctcacgc cctgtggcgg cagcccggac aaactaatcg tgtattgcgt ccagacggtc
     1081 ggatagccgt cctccgtcag atccggattg atagtcagca agagccgcgt gaaatcgccg
     1141 accgaggtcg gagtcgtgcc gacattggtg cttgcaccgt tagtcgagag gcgaagttcc
     1201 agcctgtccg ggaaggtaac attggaggtt gttcgcgtcc agaatttaat gacgccgccg
     1261 ttgtacaatg tcaaattcgg cgtgagcagc cagttgctga tggtgttttc gccggtcgta
     1321 ctcccgtaat ttgctccgat gtaagaggtt ggcgtgcccg attgagccgg aaaaacgtta
     1381 tggttgccct gaaaccagtt cagtccgacc gggttgctgt tgttctgctt aaaccacccg
     1441 cgccccggaa gcagagttac gtcgtcgaaa tcttcaaata actgcgcccg cgcattgccg
     1501 cccgccggcg caaaaatcag aacagccgcg agaaaaatca cgattcgctt tgaaaaaagc
     1561 agcaacgatt ttctgttttt agcccgaatc ataagaatta tctccttaac gagactgaag
     1621 ccgaaaagcg cttgagccga tttttctatc tgagcagatg ttgaatggaa tttactttct
     1681 ttgtttttga aagtcaagag tttttcaatt acgaattacg gattacgaat tataaataaa
     1741 aaaaggagat tcgtcggcaa aagccccgaa tctccttcat tcgtaattcg taattcgtaa
     1801 tttacttaac cgccgttgtc gtcgttttct gtctcgggtc gtacggcgtc gccatatagc
     1861 gtttgacggc ttcctgcgcg ttgtcgtaat cgtcgccgct ggcttgggcg cgcgtgtagg
     1921 cggcgacggc gcgcgtgcgg tcgcctttgg cgtcgtaggc attgcccatc ttgatattcg
     1981 cccagacttc gagccagttc ggacgcaggt ttccggaaag cgtcgccttg taattatcaa
     2041 tcgccaaatc gtaattgcgc tgttcgagaa acagattgcc gagattgtag taaatccacg
     2101 aattgttgcg gtcgagcttt aaagccgctt cgaactgctg ttgcgcttcg gcgagatttc
     2161 cctctttgaa ctgctcgatg ccgcgccgcg cgacgctcga gactttcaaa tcgtcggaaa
     2221 tgcgcaaaag cttgtatttc gggtcgatga cgacttcgag cggcttgccg ttcgattcga
     2281 tgttgaaatc ggcgctcgcg tcttcaatca gaaccgtctg cgttttcagc tcgccttcgc
     2341 cctcggcaac cagctggacg tcgaccggca ggcgcaggtt gtcgtagttt tgttttatcg
     2401 tgccgcgcgt gacgaattta ccttcgcgcg tgcggatgat ttgatattcg acggcaaatt
     2461 cgggaacgcc cgtgccttcg acccagcgcg cgaagaaata gcgcatcggc tgtccggcaa
     2521 cctgcgtcgt cagcttttca aaatcgtcaa tcgaagcgtt tttgccgcga tactgcgcta
     2581 aaaactggcg caggagctgg ctgaatttct gtgcgccgag cgtttctttc aaaaggcgat
     2641 aaaccagcgc gcccttgccg tacattatgt attgatacgc ggtcgaggta tcgtcgagcg
     2701 cggacggcgc gcgcaaaagc gatgcggtct gctcgaaggt caaagctttt tccagcgtct
     2761 cgcgctgcag agcttcgagc ttggtgttgg aaattcccgc ttcgcgcagc tccagcgcgc
     2821 tccattccgc caagccctgc gagagccacg cgtcgtcaaa cgatttcaag ccgacggttt
     2881 gtccccacca ttggtaagcg gcttcgcgct gcaatctttc ggcggcaatc tggcgcgact
     2941 ggtcaaaaaa tttgtcggcg atgaaaatca ttccgggcgc cgagtaaaaa tcgagcgttt
     3001 cgtcgtcggt ctggacgata atcaggcgct tgccgctgtc cggctcgccg tattgtttgg
     3061 tgtaaaaatc gagcgcgcgt cccagtgtct cgccataggt cgaaatgagc gcgtcgcttc
     3121 ccgctttagt gtaaaactgc aattcgtaat cggcgaaacg cagcgttttc ggcgtgtatc
     3181 tgccgtaggc gaaattgccc ggaagcgccg ccttcgattg cgtgaaacga tattttccgc
     3241 cggtcgcgga aacgggcgcg tcgctgtatc cgattatttg ataaccgctc ggcagcgtta
     3301 tcgtgatgtc ggaagtggcg cggtcagccg cgtaattgtg aaacggaaac cagcgcgccg
     3361 catacatcag ataaccgttt ttgtcgccga cgtaagcgag ccgtttggtc aggagcggtc
     3421 cgccttcggg cgtcgccagc acgccgctgt atttaaaacg aagcgtgacg ggcacgttag
     3481 ccggaatcaa atcgcccaaa tcgactttca cgctcggtcc cagatcggaa acgccgacct
     3541 gatcctgaac gaatgtaatc tggctttgcg aagcggtcga aggcgcggtc gttttgcctt
     3601 tcggcgtcgc cggcgtgatt gtcgccgccg ccgagccgac gcgcgaaatg ctttcgactt
     3661 ttaaagagcc gttcagctca aaagcgacgg tgcgcgtttc ggcgagcggc gtaaaagtaa
     3721 cgtcggcgac ggcttgaagc tgattttcgg aaggattgag ctgcacctcc attcgataat
     3781 tcgagacatc aaacggagcg cgctgaacct gctgcgcgag gacggaaaaa actgagagta
     3841 aaattacggc tgaagccagc acaagcgacg gaaagaaacg tttctgcatt tttatcacct
     3901 tttggaatgt tgctgtaaaa ctattttttg caaaactatg atgtctgaaa aattcttttc
     3961 gtttgcctgc cggcaacaga attaagtttg ttataaaagc gaaaccggat tttaaccgcc
     4021 cgatatttat ttcggcggaa ttttttcttt cgtaaattct atggcttgaa acaaatcggg
     4081 agttttcggc agaaagctga tttcgcccgg cagacgctct atttccagat aatcgtcaaa
     4141 ttcgcggtag cctttataag agctccattc ataccagttt tcctgcggag ccgtcgtcgt
     4201 gacgcccatt tcgtcgagcc gcgcgcgaaa gctccagcct ttcatttgag agactctctt
     4261 ttcccaatca agatagctgt aaacggacgc ggcaacgccc agacccgaaa gaaacagcaa
     4321 atgcagcggg attaaacgag cgacgccggc gtaagcatcg acaaaagcga cgaccagcca
     4381 caccaccatt aatttcgacg cgtaaaaata aacaatatca ttgacggctt tcgcgcgcag
     4441 cgctttccgg tagcctctgg cagccagttc gtcatcaacc tctatatgca cttccagcat
     4501 aattttaaat aaccgttgtc acgatttcgc ggatgctgcg ctgcaattcc cggtcgtcgg
     4561 taatcggcga gcacaaggcg acctcagccg cattgaccgg cgcgatgcct ttggaaatca
     4621 tctgcgccgc gtaaatcaaa agtcgcgtcg aaacgccttc ttccagaccg tgaccgcgca
     4681 ggtttctgac tttttgcccg atggtgacga ggtcgcgcgc cgtcgcttcg tcgacgccgc
     4741 cttcgcgggc gacgatttcc gtctccgatt cggcgggcgg ataatcgaat tccatcgcca
     4801 cgaatctctg ccgcgtcgat tgttttaaat cctttaaaat cgactgataa cccggattat
     4861 acgaaacgac cagcataaat tccggcggcg catcgaccac cgttccgcgt ttttcaatcg
     4921 gtagacgccg acggtcgtcg gtcagcgggt gaatgatgac gaccgtatct ttgcgcgctt
     4981 cgacgacttc gtccagatag caaatcgctc cggcgcgcac cgctttcgtc aaaggtccgt
     5041 cgtgccagac cgtctcgtcg ccttcgagca gaaatctgcc gacaaggtct gttgccgaca
     5101 aatcttcgtg acaggcgacc gttatcagcg gtctccccag acgccacgcc atatgctcga
     5161 caaaacgcgt ttttccgcag cccgtcggac ctttcagcat cacgggcagc tttgccgcat
     5221 aagcggcttg aaaaagctcg acttcctgcg ccaccggcag ataataaggc tcgctttcaa
     5281 ctttgtattt ttcaacggct aattccataa ataattgctc gactggtaaa aatttatctt
     5341 aacattcgcc cgtctttata caaaaactga aaccgccgca aaaattgcag cggcaagccg
     5401 caccgcctgc ccggctgagc gcagcgaaaa agcgtttaaa taaaatagcc gcgaaatagc
     5461 gcgaaagaca cgaaataaat gataaaaata tttcgtgtct ttgcgcgttc tttcgcggct
     5521 gatatttttt agcggctcgg cgctaaaatc ttctttttta aattattttc ccttgaacgc
     5581 gggttttcgc ttttccagaa aggcggcgac gccttcttcc ttgtcttcgg tcgaaaaaca
     5641 aatcgcgaaa agatcgactt cgcggcgcaa gccttcgtcc agattcgagc gcgaggcaag
     5701 cttgacggct tccttcgaga gctgcagcgc gatcggcgct ttctgcgcga tttttccggc
     5761 gagctccagc gttttcgttt cgagctcttc gcgcgtccag acataattga cgagcccgaa
     5821 ctgcaaagct ttcgcggcgt caatcatatc gcccgtcaga atcatctcca tcgctctgcc
     5881 ttcgccgata agacgggtca gacgctgcgt cccgccgccg ccgcacataa ttccgagatt
     5941 gatttccggc tgcgcaaacc gcgcgttttc gctgcacacg cggatgtcgc aggcaagcgc
     6001 cagctcgcag ccgccgccca gacaaaaacc gttaatcatc gcgatgaccg gcttcggaaa
     6061 cgtgtcaatc gaattaaaaa gcgtccgctc gtaaaataaa tttctctgcg acgccggagt
     6121 ctcgccggca aattccgcga tgtccgcgcc ggcgacgaaa gatttttcgc ccgctcccgt
     6181 gacgacgacg acgcacaccg ccgcgtcccg tcgcagctcg tcgagcgcgg cgacgccttc
     6241 ttcgtgaacc tttcttccga gagcattaag tttttcgggg cggttaatcg ttataatggc
     6301 aacgctttcg cgcttttcga ccaggatagt ttcgtaattt gtcatttttc gtaaatagta
     6361 aatcgcaaat cgtgaagcgt aacctgcatt tctgcaattc acgcttcacg attcacgatt
     6421 tacgaaaatt ttatttttct tcttcgtcgc tcggctggtc gtcggcaatc cacgcgacga
     6481 aaagcctgag ccagttttcc ttcgtttcgg taatggcgac gccgatgcgc gccgtcccgt
     6541 aatttgacgg cgaaacccgc acgacgcgag cctgcacctc gaccggaacg ccgtcgggtc
     6601 gatgcgagcg aatatataaa ttttcgccct gcgcgacttt ttgattcagg tgaaacagcg
     6661 ctccggtcgt cgaaatatcg tcggtttccg tcggctcgct ccacgccgcg ccgtcgtccg
     6721 cgcgtcccga tacgacaatc ggcaaccgca aagccattct cagcgcgaag cgtttttcct
     6781 catactgtgg ttgcagatta gttgacatga caagttttga agaatcgaaa actttaggta
     6841 gaacgtgcca gctagtttaa cacgaaaacc gtttaaacaa acaatggatt ttaaaaaagt
     6901 gaataaaatt taaacgcggc gaaatccata aataaaccgt tttatttatc attcgactag
     6961 gcgttctgtg atagttatac taaagcttac gcaaaaatgc caaactgccg caaaaaattt
     7021 tgtaaacttt aagaacagga gcttttgttc aagaatatgc cagaaacgga aattaatcgt
     7081 aaagtcgtta ttttaggttc gggtccggcg ggtttgaccg ccgccatcta cgcctcgcgc
     7141 gcgcagcttc agccgatggt catcgacgga ccgcagccgg gcggacaatt gacgattacg
     7201 accgacgtgg aaaattatcc gggctttgcc aaaggcatta tgggaccgga attgatgaac
     7261 gagtttcgcg cccaagccga gcgcttcggc acggaatttc tgaacgtctg gattgataaa
     7321 gtcgatttaa gtcaaagacc tttcaagctt tacggaaaag ccagcccgga cagcgccgag
     7381 atttccacca tcgtcaaagc cgaaactttg attatctcga ccggagcgtc ggcgaaatgg
     7441 ctcggcgttc cgggcgaaga acccgcgccc gccggtttgg gcggcaacgg cgtttcggct
     7501 tgcgccacct gcgacggatt tttctttcgc ggaaaagaag tggtcgtcgc gggcggcggc
     7561 gatacggcga tggaagaagc tacttttctg acgcgctttg cttcgaaagt cacgattgtc
     7621 caccgccgcg actcgtttcg cgcctcgcgc attatgcagg agcgcgcgct cgccaacgag
     7681 aaaatcaatg tcctctggaa cacggaaatc agggaaattc agggaacgaa agaagccggc
     7741 gtgaccggca tcaaaatcta taacaatcag tcgaaagaag aaaccgtttt tccgaccgaa
     7801 ggcgttttta tagccatcgg gcacaagccg aacacagacc ttttcaaaga ctggctcgaa
     7861 ctgaacgaag tcggctatat tcaaaccgaa ggacgcacga tgaaaaccaa tatcgaaggc
     7921 gttttcgcct gcggcgacgc gcaggatcat tactatcgcc aagccgtcac ggcagccgga
     7981 accggctgta tggcggcaat cgacgccgaa agatttttag ccgaacacgg cgaagccgaa
     8041 agggtgacgg cgacgaactg gtagcttttc gattacgaat tacgaattac gaattacgaa
     8101 tgattgaatc gaatcgaacg tttgatttat tcaaacccaa ttcgtaattc gtaattcgta
     8161 attttcctat tgtttcttaa ccacggctcc tttcaccgtt tcgagcttct gcggcgtttt
     8221 ctcgagcttt gccggattgt agcccgcgat ttctactttt cgcaaaatat tttgataagt
     8281 cgtctcgttg atttcgggcg tgcggcttaa aatccataaa tacttgcggt cgggattgcc
     8341 gacgacggcg tattgataat tttcgtccaa atcaatcacc cagtacggcg cggacgaaag
     8401 ccacgcaaac gagcctttca gtttcgcatt cgtttgcgtg tcgatgattt tcgccattcc
     8461 ggtcgcgtcc tcggtttttc cgtctttggt cagacattta ttgacgacct cgacctcgcc
     8521 gcccgctctg aaattataag tggcggtcgt attgccgacg cattgttttt gcgctttgtt
     8581 cgcgtatctg gcgatttcgt accacgttcc gccgtaacgc ttgacgtcga cgaacgaaac
     8641 ggttttcagt tcgcccgtct ttgtattctg agcctgcacg cacgtcgccg ccgacaccaa
     8701 agtcagggcg caaatcgcta aaatccattt tctcacttgt cgagcctccc ggttctcgtt
     8761 aagtttaata aattgcttta taaatattct gccacaattt gctttcgtcg atgcaagatt
     8821 tttataattt ttctgaaacc gtctcaaaat cactttgcga acccggcgtt ttttctttgc
     8881 gccaatcggt tgaaattttt cttaaccgca gagaacgcaa agagcggttt atgagaaaat
     8941 ctgtagttat taataattta agaggttata ataatttata tgccgattta tgaatatcgc
     9001 tgcgccgatt gtgcggcgca tctcgaaacg atgcagaaaa tttccgacgc gcctttgacc
     9061 gtctgcgaag cctgcggcgg aaaactggaa aaacaaattt cgcgctccgg gtttcagttt
     9121 aaaggctcgg gctggtatgt caccgattac tcgaacaaag gcaaaagcga tacgaaaacc
     9181 gaagccaaat ccgaaaccga ggcgaaatct gaaaccaaaa cggaaaaaag ttcggcgggc
     9241 gaatcaaccg ccggaggcga tgctgcatca aagccggcag acagttcacc caagacatct
     9301 aaaacggaat gaggaaaaat gaaattttcg gctaaaaaag cgttgttttt gagtttatgc
     9361 gttttgctga tttcagtttc ggcgcttgcc cagtcgcgcc gcagcgcgaa agatacgtcc
     9421 ggcgacggcg gaacgattct gagcgtcgtc gcggcgcgcg ccaacggttc gaccgagccg
     9481 ataaaagccg aaaatctttt tctatacgaa aacggcatcg agcagaagat aaaaaatttt
     9541 actttcgacc cgtcgccttc gaggatagtc gtgctggtcg acaattcgca gaccttgcgc
     9601 gccgaggtcg agacgctgaa aaatgcggtc aaggaattcg tttacgaaat ttacgaaggc
     9661 gaccaggttt tcgtcgtcgc ttatgacgaa acgccggaaa tcattcagga atggaccgac
     9721 gacgcgaaaa aggtcgagac ctcgctcaac acttttcgca aacagggcaa tccttaccta
     9781 ttcgacgcgc tgaacgccgc cgcccgcgaa attctgctgc cgctgatgcc cggaacgcgc
     9841 aaaaccgccg tcgtgctcat cagcgacggg ctcgaccgcg gcagcaaaac ggcttttgat
     9901 aaaattctcg gcgagctgca atcggataac atcacggttt acgcgctcca gcttcccgac
     9961 cgcacgaacg gcgcgtaccg gcgcaaccag ccgaaagccg cgcaggtaat taccgagctg
    10021 accgaaggca cgggcggcaa aattttcgac ttcgaacagc cgcaggtcgc cgccaaagcc
    10081 atctgcgacg agctgcgcaa aaaccgttat ctgctttcgt atttttcgct taacgcttcg
    10141 tccttcgatg cccggcgcgt tttcctgctc ggtcaggaag gcatcagcgt ccgcaccaaa
    10201 aacgcgca
//
LOCUS       DASWNE010000880         5539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_207034, whole genome shotgun sequence.
ACCESSION   DASWNE010000880 DASWNE010000000
VERSION     DASWNE010000880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5539
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207034"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="VGB68_17255"
     CDS             complement(<1..1078)
                     /locus_tag="VGB68_17255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0051044.1"
                     /translation="MAQENKPKILIFCDFYLPGYKSGGGMRTLVNMVDRLGEKFDFWI
                     VTRDHDGKTDKTPYKNVKINDWNEVGKARVYYLSKDNIKPKSVKTLIEDVQPKSIYAN
                     SFFSTLTVFILWLRKFRKIPNLKIIVAPEGELAPGALGLKKNKKQLYLKFARALSLYE
                     NIVWKATAEFEKEEIEKVKGKDGKIFIAPNMPPKQIFPEFNLKNKPYKISGELNAVYL
                     SRLHPIKNLKFLLEILPNIKDKVYLDIFGPVDNHQYVEECEQLIKKLPKNLSVQMKGE
                     VSHENVMKTLEKYNFFVLPTQGESFGHIIAEALASGCPLIISNKTPWTNLEEKKIGWD
                     LPLDDKNRWIDTLKYCIALDNDSYS"
     gene            complement(1062..2132)
                     /locus_tag="VGB68_17260"
     CDS             complement(1062..2132)
                     /locus_tag="VGB68_17260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEY0051045.1"
                     /translation="MDKILVYRIGSIGDTLVALPSFWAIRNAFPQAHITYFTNGETSD
                     PNDLIAKKVLPEGIFDEWLVYPNDFAKASAGLILKLRRKKFDSLFYLMTRNRGYYRVK
                     RDVLFFRLAGIKKFFGVNYLLENLLTMAPEKPVKTVESELEYLLKSLYFENLELPPID
                     SFKPNLKLTFDEIKKSRDWLESNCPAAWREKRLIAVMAGTNWSSKIWAEENYIEIVSR
                     LIKEKNVFPVVFGGKNDREQGDRFLEAWKTGANAAGELGIRGDAGLLRECNLYLGTDT
                     GTMHLAGSVGTPCVAVFAAIDYVGRWTPFGSGHRIFRERVECEGCFAPVCFNRHKCLN
                     LISPQSVFRACAEILENNGSGK"
     gene            complement(2142..3113)
                     /gene="rfaE1"
                     /locus_tag="VGB68_17265"
     CDS             complement(2142..3113)
                     /gene="rfaE1"
                     /locus_tag="VGB68_17265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HEY0051046.1"
                     /translation="MENFDKLIARFSKVKILIVGDVMLDRYWWGSVSRISPEAPVPVV
                     QLEKTSLAAGGAANVAVNVSTLGAVPRLIGVIGGDEEGKYLSDALDKSKISPDLLLTL
                     KERPTTVKTRIVAHNQHVVRIDNEKTDFLTSEQEEALWLSIESELDKCDLILISDYAK
                     GLLSKNLLKRLITKAAVNNKHVLVDPKGKDYTKYSGATLLTPNRREAIEACHFEEHQV
                     ADDITIAGETLISELNLEALLITQGEKGMTLFQKEEMPVHFQTKARTVYDVTGAGDTV
                     IATLAVALAAGANLLQAAELSNISAGLVVEEVGTTTINQEKLLKALT"
     gene            complement(3120..4136)
                     /gene="gmhA"
                     /locus_tag="VGB68_17270"
     CDS             complement(3120..4136)
                     /gene="gmhA"
                     /locus_tag="VGB68_17270"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR00441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HEY0051047.1"
                     /translation="MEKVVFTNGCFDILHPGHIDLLERARRLGTKLIVGINSDKSVRE
                     IKGSPRPFLSQEARASILRALRCVDEVRIFEEPTPAKIIEQIKPDVLVKGGDWQVEQI
                     IGADFVLKRGGEVYSLALKDNFSSSAIVEKIQSDTNGDKDITNSLNDLVSTSLNEHAE
                     VFRKVLESETERIEKCADIIFEAFADGKKILICGNGGSAADAQHIAAEFVGRYETERR
                     GLPAIALTTDTSALTALSNDYGFERIFSRQVEALASAGDVLIGFSTSGNSPNVISAVM
                     KAREIGCKTIGLTGIDGKKLASLCDACILVPSRRTARIQEAHITIGHIWCEIIDSKFK
                     EGEF"
     gene            complement(4162..>5539)
                     /locus_tag="VGB68_17275"
     CDS             complement(4162..>5539)
                     /locus_tag="VGB68_17275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051048.1"
                     /translation="YLMPWCLLTGAVIAAPSLYLYYKNSFNLFHPLVFAAWSYFFPAF
                     FVGGFVLSAGFSQPFFLNFVQDERYNLPLTLVYVMLGYIGLSVGFFLPFGRKIGERIN
                     RWMPEWNWKPEQLLAPGLLLLAIGLFNTIFAFAAGVIGFQQVEEIGIFDGTIFLLTLF
                     WLEAAFLLWLMVFRTKELNFNHYLVIGLLLVISLGKSAIQGNRGSLIQVFILIAFAFV
                     SSGRTIKFQHKMLGGILLALALLVGMIYGTTFRNIKQSQERVSIDEYVGNIFEAFGKI
                     SDQGVFASMEEGFVALAERIDAVSSLAVVVSNYEQLAPYEESYGLDNNIWKDSITFLI
                     PRVVWNDKPLATDPSKYGDLYFNFSENSFTLTPIGDLLRNFGPVGIPLGMILLGFFIR
                     IFYACLIENQSVSFWRATVYYMLLTSISYEGSYGLIIPYLIKVGFIAVLGLLIVWFFV
                     RNSSYKRS"
BASE COUNT         1444 a         1424 c         1167 g         1504 t
ORIGIN      
        1 cactgtaact atcgttatct aaagcgatgc aatatttcaa tgtatctatc cacctgtttt
       61 tatcgtcgag cggcaaatcc caacctattt tcttctcttc caaatttgtc cagggagttt
      121 tgttgcttat tattaaagga cagcctgaag ccaaagcctc ggcgataata tgcccgaagc
      181 tttctccctg tgtgggcaaa acaaaaaaat tatacttttc cagcgttttc attacatttt
      241 cgtgcgaaac ttcgcctttc atctgtacgc tcaaattttt cgggagtttt tttataagct
      301 gttcgcactc ctctacgtat tgatgattat cgaccggacc gaaaatatcc aaataaactt
      361 tatctttaat gtttggtaat atttccagca aaaacttcaa gttctttatc gggtgcagtc
      421 gggacaaata caccgcattc agctcgcctg agattttata aggcttattt ttcaggttga
      481 attcgggaaa aatctgcttt ggaggcatat tcggcgcgat aaaaatcttt ccgtctttcc
      541 cttttacctt ctcaatctct tctttttcga attcggcggt cgctttccaa acgatatttt
      601 catacagact gagcgcgcgg gcgaacttta gatacaattg tttcttattt tttttcagcc
      661 cgagtgcgcc cggcgcgagc tcgccctcgg gcgcgacgat tatcttcaaa ttcggtatct
      721 ttcgaaactt tctcagccat aaaataaaaa ccgtaagcgt cgagaaaaaa ctgtttgcat
      781 agatagattt aggctgtacg tcttcaatta atgtcttaac cgatttcggc tttatattat
      841 ccttcgataa ataataaacc cgcgcttttc cgacttcgtt ccaatcattt atttttacgt
      901 ttttatatgg agttttgtcg gttttgccgt cgtggtcgcg cgtaacaatc caaaaatcga
      961 atttttcgcc gagccggtca accatattta cgagcgtgcg cataccgccg ccgcttttat
     1021 agcccggtaa ataaaaatcg caaaaaatca gaatcttcgg tttattttcc tgagccattg
     1081 ttttccagaa tctccgcgca ggctcgaaag acgctttgcg gcgaaatcaa atttaaacat
     1141 ttgtgccggt tgaaacaaac cggcgcaaaa cagccttcgc actcaacccg ctcacgaaag
     1201 attcggtgcc cgctgccgaa aggcgtccag cgcccgacat aatcgatggc tgcgaagacc
     1261 gccacgcagg gcgttccgac cgaaccggcg agatgcatcg ttccggtgtc ggttccgaga
     1321 tataaattac attcccgcaa aagtccggca tcgccgcgaa tccccaattc gcccgcagca
     1381 tttgcgcccg tcttccacgc ttcgagaaat ctgtcgccct gctcgcggtc gttttttccg
     1441 ccgaagacga ccggaaaaac atttttttct tttataagcc gggaaacaat ctctatataa
     1501 ttttcttccg cccaaatctt tgagctccaa tttgttccag ccattaccgc aatcaatctt
     1561 ttttcacgcc aagccgccgg gcaattcgat tccagccaat cacgcgactt tttgatttcg
     1621 tcgaaggtta gcttcaaatt cggtttgaag ctatcgatcg gcggcaattc cagattttca
     1681 aaatagagac ttttaagcaa atactcgagc tcgctttcga ccgttttgac gggcttctcg
     1741 ggcgccatcg tcaacagatt ttccagcaga tagtttacgc cgaaaaactt ttttattccc
     1801 gctaatctga aaaacagaac atctcttttc acgcgataat aaccgcgatt tctggtcatc
     1861 agataaaata acgaatcgaa ttttttccgc cgaagcttta aaattaagcc tgccgaagct
     1921 tttgcaaaat cgttcggata caccagccat tcatcaaaaa tcccttccgg caaaaccttt
     1981 ttcgcaatca ggtcgttcgg gtcgctcgtc tctccgttgg taaaatatgt aatgtgcgcc
     2041 tgcggaaacg catttctgat tgcccagaac gacggcagcg cgacgagcgt atcgccgatg
     2101 ctgccaatcc ggtaaactaa tattttatcc atccgggaaa attaagttaa agcctttaga
     2161 agcttttcct ggttaattgt tgtcgttccg acctcttcga ccaccaagcc ggcgctgata
     2221 ttcgacaact ccgccgcctg cagaagattc gcgcctgccg ccagcgcgac tgctaaagta
     2281 gcgataaccg tgtcgcccgc gcccgtaaca tcgtaaaccg ttcgcgcttt cgtctgaaaa
     2341 tgaaccggca tttcttcttt ttgaaataag gtcattccct tttcgccctg cgttatcagc
     2401 aaggcttcca gattaagctc ggagattaaa gtttctccgg ctattgtaat atcgtcagcc
     2461 acctgatgtt cttcaaaatg acaggcttct atcgcctcgc gccggttcgg agttaaaagc
     2521 gtcgcgcccg agtattttgt gtaatccttt cctttcgggt cgactaaaac gtgtttgtta
     2581 tttactgccg ctttcgttat cagacgcttg agcagatttt tactgagcaa tcctttggcg
     2641 taatccgaaa ttaatattaa atcacattta tccagctcgc tttcgattga aagccagagc
     2701 gcctcctcct gttcggaggt caaaaaatct gttttttcat tgtcgattct gacgacgtgc
     2761 tgattgtgcg cgacgattcg cgtcttgacg gttgtcgggc gctcttttag agtgagcagc
     2821 aaatcgggtg aaatttttga tttatcgagc gcgtctgaca gatattttcc ttcttcgtcg
     2881 ccgccgatga cgccgatcaa ccgcggaacg gctcccagag tcgacacatt gaccgccacg
     2941 tttgccgcgc cgccggcggc gagactggtt ttttccagct gcacgaccgg caccggcgct
     3001 tcgggcgaga ttcggctgac acttccccac caataacggt cgagcattac atcaccgaca
     3061 attaatattt ttactttaga aaagcgagcg ataagtttgt cgaaattttc cacttctgtc
     3121 taaaactcgc cttccttgaa ttttgaatct atgatttcgc accagatgtg accgatggtg
     3181 atgtgcgctt cctgaatccg ggcggtgcgc ctgctcggaa ccaaaatgca ggcgtcgcaa
     3241 agcgaagcta gtttttttcc gtcgatgccg gtcaaaccga tggttttgca gccgatctct
     3301 ctcgctttca taacggctga aatgacgttc ggcgaatttc cgctggtgct gaaaccgatt
     3361 aaaacgtcgc cagccgaagc taatgcttcg acctgtcggc tgaaaattct ttcaaatccg
     3421 taatcgttcg acagcgccgt caaggcggaa gtgtccgtcg tcaaagcaat cgcgggcagc
     3481 ccgcgccgct cggtttcgta ccgcccgacg aattcagccg cgatatgctg cgcgtccgcc
     3541 gcactgccgc cgttgccgca aattaaaatt ttctttccgt cggcaaacgc ttcaaaaata
     3601 atgtctgcgc atttttcaat ccgctccgtc tcggattcca gaacttttcg gaaaacctca
     3661 gcgtgttcat tcaaagacgt tgaaactaaa tcgtttagcg aatttgtaat atctttatcg
     3721 ccatttgtgt cgctctgaat tttctcgacg attgcgctgg acgaaaaatt gtctttcaat
     3781 gccagcgaat aaacttcgcc gccgcgtttc aatacaaaat ccgcgccgat tatctgttcg
     3841 acctgccaat cgccgccttt cactaaaacg tcgggcttta tctgctcgat aattttcgcc
     3901 ggcgtcggct cttcaaaaat ccttacttcg tcgacgcaac gcaaggcgcg aagaatcgaa
     3961 gcgcgcgctt cctgcgataa aaacgggcgc ggcgagcctt ttatctcgcg caccgattta
     4021 tcgctgttta tcccgacgat aagctttgtt cccagacggc gcgcgcgttc cagcaaatcg
     4081 atatgtcccg gatgaagaat gtcgaaacag ccgttggtaa atactacttt ttccatcgaa
     4141 ttaaagactt atgctttaag gctaacttct tttataactt gagtttctga caaaaaacca
     4201 aacgattaac aatcccagaa cggcgataaa accgaccttt atcaggtaag gaataatcaa
     4261 tccgtacgag ccttcatagg aaatactggt taaaagcata tagtaaacgg tcgcgcgcca
     4321 gaaggaaaca ctttgatttt caatcaaaca ggcataaaat atgcgtataa aaaatcccag
     4381 aagaatcatt cccagcggaa ttccgaccgg accgaaattt ctcagaaggt cgccgattgg
     4441 cgtcagcgta aaggaatttt cgctgaagtt gaaatataaa tcgccatatt tggacgggtc
     4501 tgtcgcgagc ggcttatcgt tccagacaac gcgcggaatc aaaaacgtaa tactgtcttt
     4561 ccagatatta ttatccagac cgtagctttc ctcgtaaggc gctaattgtt cataatttga
     4621 gacgacgact gccagcgagc tgacggcgtc tattctttcc gccaaagcta caaaaccctc
     4681 ttccatactg gcgaacacgc cctgatcgga aattttcccg aaagcctcga aaatgtttcc
     4741 gacgtattcg tcaatcgaaa ctcgttcctg cgattgtttg atgttgcgaa aggtcgttcc
     4801 gtaaatcatt ccgaccagca gcgcgagcgc taaaagaatt ccgcccagca ttttgtgctg
     4861 aaattttatg gttctgcctg acgatacaaa cgcgaaagca ataagaataa aaacctgtat
     4921 caggcttccg cgatttccct gtatagcgga tttgcccagc gaaattacca gcagcaagcc
     4981 gataacgaga tagtgattga agttaagctc tttcgtccgg aaaaccatca gccacaataa
     5041 aaatgcggct tccagccaga agagcgtcaa aagaaaaatc gtcccgtcaa aaatgccgat
     5101 ttcttcgacc tgctgaaaac cgatgacgcc ggcggcaaag gcgaaaatgg tgttaaacaa
     5161 cccgattgcc agcagaagca gaccgggagc caataactgc tccggtttcc agttccattc
     5221 cggcatccag cggttgattc tttcaccgat ttttctgccg aagggcaaaa agaatccgac
     5281 cgataaaccg atgtagccga gcatcacata aaccagcgtc agcggtaaat tgtaacgttc
     5341 gtcctgaacg aaatttaaga aaaaaggctg agaaaatccg gctgataaaa caaaaccgcc
     5401 gacaaaaaac gccggaaaaa aatatgacca cgcggcaaac acgagcgggt gaaaaagatt
     5461 aaagctgttt ttgtaataca gatacaggct cggcgccgcg atcaccgcac ccgtcagcag
     5521 acaccagggc atcagataa
//
LOCUS       DASWNE010000881         5021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_29353, whole genome shotgun sequence.
ACCESSION   DASWNE010000881 DASWNE010000000
VERSION     DASWNE010000881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5021
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29353"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VGB68_17280"
     CDS             complement(<1..294)
                     /locus_tag="VGB68_17280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051049.1"
                     /translation="MNIQFSPGKIKNTLSLVFIGGVFLCVTLGCSLLKNQRGARHPGW
                     KMQEACAYFSDVNLKFTGEPTNELCRGGGFSRADGLLYHQGSPENIGSISYRVE"
     gene            complement(376..2352)
                     /locus_tag="VGB68_17285"
     CDS             complement(376..2352)
                     /locus_tag="VGB68_17285"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: protein motif:HMM:NF008559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HEY0051050.1"
                     /translation="MTRSRRRNLFCLQIAVLLCFLLCASALQAAATHPLDPLDENEIT
                     SAVKILQSAANFPKTALFSTVVLNEPPKAEVLSFKTGAKFRREAFAIVLDREKNKTFE
                     AVIDLRSGKILSWKEIAGVQPLVFLEEYEVLPDIVKADKRWQEAMRKRGITDFEKVAV
                     DGWAVGQAPTQLSGRLLRALSYFKGDAANYYGRPIEGVVAIVNMNTRAVVDVTDTGVV
                     PVSKTSQDFDEKSVGKLREKPKPLLITQPEGASYQIIGNEVAWQKWRFRYTMHPREGL
                     VLHTIGYDDEGKTRPILYRASLSEMVVPYGDPDENWRWRAAFDVGEYSVGRLASPLEP
                     KLDAPENAQLIDATFADDSGKPYVLEKSVGIYERDGGILWKHYDSVAGKNETRRAREL
                     VMFFVATIGNYDYAVNYVFKQDGSLEVDLALTGIMLAKGVREKRADENHQMMNDGMSG
                     HLVAENIVAPHHQHFFSFRLDFDVDGVNNSVTEMNTSAMPAGANNPYLNGFVMRESVF
                     KKETEAQRKMDMQSARVWAVMNPSAKNSLGHNTSFILVPGANSLPYIAPEALVRKRAQ
                     FINNHFWATRYNAAEMFAAGVYPNQSKGGDGLPQFVANDEALENTDVVVWYTLGVTHI
                     PRPEEWAVMPVTHVGFKMIPGGFFSRNPGLDVPR"
     gene            complement(2471..3838)
                     /locus_tag="VGB68_17290"
     CDS             complement(2471..3838)
                     /locus_tag="VGB68_17290"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY0051051.1"
                     /translation="MARKAILVVDDEKSQREILEMILSGEGYDVTTASSGEAALKFVA
                     DRRFDLVLSDLKMTGMSGIDLLKELVAYDKSIMVILLTAHGTVDSAFEAKDMGAFAYL
                     QKPYDREKLLETVSRALNKLTQLDTEIVSVSPEMDKVKKLILKVAKSNSTVLIRGESG
                     TGKELIARSIHQNSPRASEVFQAVNCAAINENLLESELFGHEKGSFTGAVGEKKGLFE
                     VADAGTLFLDEIGELDISLQAKILRALQERQIRRVGGTRELNVDVRVVAATNRDLLKM
                     TQDGRFREDLYYRLNVLSIEIPPLRERQSDIPVLIDYFIGKHTKNSSQKIKGLAADAR
                     KLLLDYAYPGNVRQLESAIERAVLLAEGDTITLDDLPPEMQSNERAGATGGGGDGTNF
                     KLPANGINFEEVERGLILQAMDRTDYNITKAAKLLGLTFRTLQYRLEKFGIKRDGDAA
                     EEAEE"
     gene            4008..5003
                     /locus_tag="VGB68_17295"
     CDS             4008..5003
                     /locus_tag="VGB68_17295"
                     /inference="COORDINATES: protein motif:HMM:NF033852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin fulvocin C-related protein"
                     /protein_id="HEY0051052.1"
                     /translation="MNKKYNLALFTLAFAAICGLFASNIFAQKTDSPKSEPTTKQCSG
                     EKFVMPQSIAVGSDAFDNYLKIASLSGYERKNAFGKLSNEQKASFIKVNLALQFVKRP
                     AMTKEQREFVLDTISKVSADIYDNSNPEKAGSREQSEMEIVNKAFGLFSRQEAGDFVE
                     PLNMNKAVEIGLLQKYNDLLKNGDITRRKIAKEMPVNDRVNIWKVQMAYHLATGKFSK
                     AQNEFILEWLTSLSPETFAPRTNLTAEEEAKALEALESRVFSIFTKPEGYAIFMTIGI
                     QKFVADDNLLEQPSCNCRWYCGLTGSCNGQCANKTKICGPSDNEECTARCVYPNN"
BASE COUNT         1190 a         1351 c         1234 g         1246 t
ORIGIN      
        1 ttcgacccgg tagcttatcg agccgatgtt ttccggcgag ccttgatgat ataaaagtcc
       61 gtcggcgcgc gagaacccgc cgccgcggca taattcattg gtcggctcgc ccgtaaattt
      121 caaattcacg tcgctgaaat aagcgcacgc ttcctgcatt ttccaacccg gatggcgtgc
      181 gccgcgctgg ttttttaaca gcgaacagcc gagtgtgaca cacagaaaaa ctccgccgat
      241 aaagacgaga gaaagtgtat ttttaatttt gcctggcgaa aattggatgt tcatcggtaa
      301 atcttcctca gtttgttttt gctcgcccgg ggcggcgcaa tccgaaaaat cggcttaaaa
      361 tttcccgcga taaacttatc gcggcacgtc caatcccgga ttgcggctga aaaatccgcc
      421 gggaatcatt ttaaagccga cgtgcgtgac gggcattacc gcccattctt ccggtcgcgg
      481 aatatgcgtg acgccgagcg tgtaccagac gacgacgtcc gtgttttcga gcgcttcgtc
      541 gttggcgaca aactgcggca agccgtcgcc gcctttgctt tgattcggat aaactccggc
      601 ggcaaacatt tccgccgcgt tataacgcgt cgcccagaaa tgattgttga taaactgagc
      661 gcgtttccga acgagcgctt cgggcgcgat atacggcagc gaatttgcgc ccggaactaa
      721 aataaagctc gtattgtgtc ccagagaatt tttcgccgac ggattcatta ccgcccagac
      781 gcgagccgac tgcatatcca tttttcgctg cgcttccgtc tcttttttga aaacgctctc
      841 gcgcatcaca aaaccgttta gataaggatt attcgcgccg gcgggcatcg cgctcgtgtt
      901 catttcggtc acggaattat tcacgccgtc gacgtcgaaa tccagacgaa agctgaaaaa
      961 atgctggtga tgcggcgcga cgatgttttc ggcgacgaga tgtccgctca ttccgtcgtt
     1021 catcatttga tgattttcgt cggcgcgttt ttccctgacg cctttcgcca gcataattcc
     1081 ggtcaacgcc aaatcgactt cgagcgagcc gtcctgtttg aaaacgtaat tgacggcgta
     1141 atcgtaattg ccgatggtgg cgacgaaaaa catcaccagc tcgcgcgcgc ggcgcgtttc
     1201 gtttttgccc gcgacgctgt cgtaatgctt ccacaaaatt ccgccgtcgc gttcataaat
     1261 tcctaccgat ttttccagaa catacggttt tcccgaatcg tcggcaaaag tcgcgtcaat
     1321 caactgcgcg ttttccggcg cgtcgagctt cggttcgagc gggctggcga gccgcccgac
     1381 gctgtattcg ccgacgtcga acgccgcccg ccagcgccag ttttcgtccg ggtcgccgta
     1441 cggcacgacc atctcggaaa gcgacgcgcg atacaaaatc ggtctggttt ttccctcgtc
     1501 gtcgtaaccg atggtgtgca gaaccaagcc ttcgcgcggg tgcatcgtat agcgaaaacg
     1561 ccatttctgc cacgcgacct cgtttccgat gatttgatag ctcgcgcctt cgggctgcgt
     1621 aatcaataaa ggcttcggct tttcgcgcag ttttccgacc gatttttcgt caaaatcctg
     1681 gctggttttg gaaacgggaa cgacgcccgt atccgtcacg tccacgaccg cgcgcgtgtt
     1741 catattgacg atggcgacga cgccttcaat cgggcgaccg taataatttg ccgcgtcgcc
     1801 tttgaaatac gaaagcgcgc gcaaaagtct gcccgaaagc tgcgtcggcg cttgtccgac
     1861 cgcccagccg tcgaccgcga ctttttcaaa atccgtgatg ccgcgtttgc gcatcgcttc
     1921 ctgccagcgt ttgtcggctt tcacgatgtc gggcaaaact tcgtattctt ccagaaaaac
     1981 cagcggctga acgcccgcaa tttctttcca cgagaggatt ttgccgcttc ttaaatcgat
     2041 taccgcctcg aatgttttat ttttctcgcg gtctaaaacg atggcgaaag cctcgcggcg
     2101 aaactttgcg ccggttttaa agcttaaaac ttccgctttc ggcggctcgt tcagaacgac
     2161 cgtggaaaaa agcgcggttt tcggaaaatt cgccgcggat tgcaggattt tgaccgcgct
     2221 tgtaatctcg ttttcgtcga gcgggtcgag cggatgcgtc gccgcagcct gcaaagccga
     2281 agcgcacaat aaaaaacaca ataaaacagc tatttgtaaa caaaacagat tccggcgacg
     2341 gcttcttgtc attcttccta attattctcc aaaaaaattt aaaacaggat gggcaggata
     2401 tacaggataa atcaaattca ttatcctgta tatcctgcct atcctgttct aattgccttt
     2461 ggcgaattta ttattcttcc gcttcttccg ccgcgtcgcc gtcgcgcttg atgccgaatt
     2521 tttccagccg gtattgcagt gtccgaaacg tcaagccgag cagtttcgcg gctttcgtga
     2581 tgttgtaatc ggttctgtcc atcgcctgca aaatcaagcc gcgctcgact tcctcgaaat
     2641 taatcccgtt cgccggcagt ttaaaattcg tgccgtcgcc gccgccgccg gttgcgccgg
     2701 cgcgctcgtt tgactgcatt tcgggcggca aatcgtcgag cgtaatcgtg tcgccttccg
     2761 ccagcagaac ggcgcgctcg attgccgatt caagctggcg aacgttgccc ggataagcgt
     2821 aatcgagcag cagcttgcgc gcatccgcag ccaagccttt aattttctgc gagctgttct
     2881 tcgtgtgttt gccgataaaa taatcaatca ggaccggaat gtccgattga cgctcgcgca
     2941 aaggcggaat ctcgattgaa agcacgttca ggcgataata taaatcttcg cgaaagcgtc
     3001 cgtcctgcgt cattttcagc aaatcgcggt tggtcgccgc gacgacgcgg acatcgacgt
     3061 tcagctcgcg cgtgccgccg acgcgccgga tttgtctctc ctgcagggcg cgcaggattt
     3121 tggcttgcag agaaatatcg agctcgccga tttcgtccag aaacagagtg ccggcgtcgg
     3181 cgacctcaaa caagcctttc ttttcgccga ccgcgcccgt aaacgagcct ttttcgtgtc
     3241 cgaaaagctc cgattccagc aggttttcgt taatcgcggc gcagttgacc gcctgaaaga
     3301 cctcgctcgc gcgcgggctg ttttgatgaa tcgagcgcgc aatcaattct tttcccgttc
     3361 ccgattcgcc gcgaatcaaa acggtcgaat tcgatttcgc cactttcaga atcagctttt
     3421 tcaccttgtc catttccggc gaaaccgaga cgatttccgt gtccagctgc gtcagcttat
     3481 tgagcgcgcg cgagacggtt tcgagcagtt tttcgcggtc gtacggcttt tgcagatagg
     3541 cgaacgcgcc catatctttc gcctcgaacg ccgaatcgac cgtgccgtgc gccgtcagca
     3601 gaatgaccat aatcgattta tcgtaggcaa ccagctcttt caataaatcg atgccgctca
     3661 ttcccgtcat tttcaaatcc gacagaacga ggtcgaagcg ccggtcggcg acaaatttca
     3721 aagccgcttc gccgctcgac gcggtcgtca cgtcgtagcc ttcgcccgac agaatcattt
     3781 ccagaatttc gcgctgactt ttctcgtcgt caaccactaa gattgctttt cttgccataa
     3841 tgttcacatc taatgtaaaa aatccttgtt atctgattcc agtttataat atagtgttcg
     3901 ctattagtaa tgctcaggca agcgcccatt cttgcttccg ctctcaaaag gctcttccat
     3961 ccgcgttatt taacttgaag tctgaaacca ctaaggagga tagttaaatg aataagaaat
     4021 acaatttagc gttatttacg ctcgcttttg ccgcgatttg cggtcttttt gcttcaaaca
     4081 ttttcgcgca gaaaacggat tcgccgaaaa gcgagccgac gacgaaacaa tgttccggcg
     4141 agaaattcgt tatgccgcaa agcatcgcgg tcggcagcga cgcgtttgat aattatttga
     4201 aaatcgcgtc gttgtcggga tacgagagaa aaaatgcttt cggcaaactg tcaaacgagc
     4261 aaaaagcgtc ttttattaaa gtcaacctgg ctttgcaatt cgtcaagcga ccggctatga
     4321 cgaaagagca gcgggaattc gttctggaca ccatctcgaa agtttccgcc gatatatacg
     4381 acaactccaa tcccgaaaaa gcaggaagcc gcgaacaaag cgagatggag attgtaaata
     4441 aagccttcgg actgttttcg cggcaggaag cgggcgattt tgtcgagcct ttgaatatga
     4501 acaaagcggt agaaatagga ctcttgcaaa aatacaacga tttgctgaaa aacggggaca
     4561 taacgagaag aaaaatcgct aaggaaatgc ctgtcaacga ccgggtaaat atctggaaag
     4621 ttcagatggc gtatcatctg gcgacgggta aattttcaaa agcgcaaaac gaatttattt
     4681 tagagtggct gacatcttta tcgcccgaaa cctttgcccc gcgcacaaat ctgacggctg
     4741 aagaagaagc taaagccctt gaagcgttgg aatcgagagt attcagcatt ttcacgaaac
     4801 ccgaaggcta cgcgattttt atgaccatcg gcatccagaa attcgttgcc gacgataacc
     4861 ttctcgaaca accttcttgt aattgccgct ggtattgcgg gctgaccgga agttgcaacg
     4921 ggcaatgcgc gaataaaaca aaaatatgcg gacctagtga taatgaggaa tgcaccgcca
     4981 gatgcgttta ccctaataac taacgcggga aaaataaaaa a
//
LOCUS       DASWNE010000882         2187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_313200, whole genome shotgun sequence.
ACCESSION   DASWNE010000882 DASWNE010000000
VERSION     DASWNE010000882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313200"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..538
                     /locus_tag="VGB68_17300"
     CDS             41..538
                     /locus_tag="VGB68_17300"
                     /inference="COORDINATES: protein motif:HMM:NF003819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEY0051053.1"
                     /translation="MAKENSFDIVSKTDYAEITNALNQTVKEVSQRFDFKGSKATVEL
                     QDKDLVLSAEDETRLRNMNDILQGKLIKRGISLKALDYQKIEPAAGGTVRQTVKIQQG
                     IPIEKAKEIVKYIKDAKLKVQASIQGETVRVSGKDRDTLQDTIAKLKAKDFGIDMTFD
                     NYRSN"
     gene            553..1239
                     /locus_tag="VGB68_17305"
     CDS             553..1239
                     /locus_tag="VGB68_17305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051054.1"
                     /translation="MFKSLIAFLLTALIATGAAAQEPNPVSPSTLTDKILNELKLDAT
                     TPEDAVRLLGKPNKDEFDDFSLGGKHGMFQVSIERIIQTKENEKAYRKLLYKKLGGTD
                     DLTLRFYEGKLVHIIFDYNLGRKEKNIPAYSLSEKYGVDFIILQGIAKDSKLSDFEGQ
                     KENTVPKVYPVLYTLLSVQKDKIYFVRVENNNSKAFWRTLISKPTKEMFPGYVLEMHL
                     ISRALTQKSK"
     gene            1249..2115
                     /locus_tag="VGB68_17310"
     CDS             1249..2115
                     /locus_tag="VGB68_17310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEY0051055.1"
                     /translation="MKSLDYYWKALRWTLHHYRPEHDVTIKTDNGLLSCSSRDWLIGK
                     HLFVRRNYELDFIKSSIEFLQSEGFLKTGKNRAVMDIGANLGMICIALLRENFFEKAI
                     AFEPSPRNFRLLRKNVRQNDLSGKIECFPMALSSENKKIEFEIDETNSGDSRVRKTAG
                     SGELKEHKRRTVSVEAKTFDAFLRENKTIEPDEIDLFWIDIQGHEGHFFKGAREFFQK
                     HKIPVVNEFWGYGILRSGITREEYCETVCATFEKFYYWTENQFQVKSTTEIEKLFERY
                     SKPREIANIILV"
BASE COUNT          671 a          490 c          539 g          487 t
ORIGIN      
        1 cgattttcaa taaagggcgg gcagtttact ataaatcatt atggcaaaag aaaattcatt
       61 cgacatcgtt tcgaaaaccg attacgcgga aataaccaac gccttaaacc agacggttaa
      121 ggaagtctcg cagcgctttg actttaaagg cagtaaagcg acggtcgaat tgcaggacaa
      181 agacctcgtt ttatcagccg aagacgaaac ccggctgcgc aatatgaacg acattttgca
      241 gggcaagctc atcaagcgcg gaatttccct gaaagctctg gattaccaga aaatcgagcc
      301 ggcagcgggc ggaaccgtcc ggcagacggt aaaaatccag cagggaatcc cgattgagaa
      361 agcaaaggaa atcgtcaaat acatcaagga cgcgaagctg aaggtgcagg cttcgattca
      421 gggcgaaacc gtgcgcgtct ccggcaaaga ccgggacact ttgcaggaca cgattgcgaa
      481 attgaaggca aaagatttcg gcatcgatat gacgtttgac aattatagaa gtaattaagg
      541 caggttttcg ttatgttcaa atcgctaatc gcctttttgc tgacggctct gatcgcgacc
      601 ggcgcggcgg ctcaggaacc gaatccggtt tcgccttcaa ctttgacgga taaaatttta
      661 aatgaattaa agctggacgc gaccacgccg gaagatgccg tccgactgct cggaaagccg
      721 aataaagacg agtttgacga tttcagcctg ggcggaaaac acgggatgtt tcaggtcagc
      781 atcgagcgca ttattcaaac gaaggaaaac gaaaaggctt accggaaatt actgtataaa
      841 aaactcggcg ggacggacga cctgacgctg cgtttttatg aaggcaagct cgttcacatt
      901 attttcgatt acaatctggg acgaaaggaa aaaaacattc cggcatacag cctttcggag
      961 aaatacggcg tggattttat aatccttcag ggcatcgcga aagattcaaa gctgtcggat
     1021 ttcgaagggc agaaggaaaa caccgtaccg aaagtttatc cggttcttta cacgctttta
     1081 agcgtgcaaa aagacaaaat ttactttgtc cgcgtcgaaa ataataattc gaaggctttc
     1141 tggcgcacgc tcatcagcaa gccgaccaaa gaaatgtttc cgggctatgt gctggaaatg
     1201 cacttgatca gccgcgctct gacgcaaaag tcgaaataaa aatatttagt gaaaagcctc
     1261 gattattact ggaaagccct gcgatggacg ctgcatcatt atcgccccga acacgacgtg
     1321 accatcaaaa ccgataacgg tttattaagc tgcagcagcc gcgactggct gataggaaaa
     1381 catctttttg tccggcgcaa ttacgaactg gattttatca aaagctcgat tgagtttctc
     1441 caaagcgaag gctttttgaa aaccggaaaa aaccgggcgg taatggacat cggcgcgaat
     1501 ctcggaatga tttgcatcgc tcttttgcgc gagaactttt ttgagaaagc aatcgctttc
     1561 gagccgagcc cgcgtaattt ccggcttttg cggaaaaacg tccgtcaaaa cgatttgagc
     1621 ggcaaaatcg aatgttttcc gatggcgctc tcgtcggaaa ataaaaaaat cgagtttgaa
     1681 atcgacgaaa caaactcggg cgacagccgc gtgcggaaaa ccgccggaag cggcgagctg
     1741 aaggaacaca aacgccggac ggtttcggtc gaggcaaaga ctttcgacgc gtttctgcgc
     1801 gaaaataaaa caatcgagcc ggacgagatc gatttattct ggattgacat acagggtcac
     1861 gaaggacatt ttttcaaagg cgcgcgcgag tttttccaaa agcataaaat tccggtcgtc
     1921 aacgaattct ggggctacgg aatcctgcgc tccggcatca cgcgcgaaga atactgcgaa
     1981 accgtctgcg ccacattcga aaagttttat tactggacag aaaaccagtt tcaggtaaaa
     2041 tcaactacgg aaatcgaaaa acttttcgag cgttacagca aaccgcgcga gattgcgaat
     2101 attatcttag tgtgagaaga acggaaaacg gagaacggaa aacggaaaat gccggttttc
     2161 aaattttcca ttttccactt tccattt
//
LOCUS       DASWNE010000883         3094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_100467, whole genome shotgun sequence.
ACCESSION   DASWNE010000883 DASWNE010000000
VERSION     DASWNE010000883.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100467"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(453..1829)
                     /locus_tag="VGB68_17315"
     CDS             complement(453..1829)
                     /locus_tag="VGB68_17315"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY0051056.1"
                     /translation="MKLRLIFIAFTVLFLTVNLRAAVLTVSTLDDSDDAACDAHCSLR
                     EAVKFAVAGDTIIFDRVLRGGTIQLTRTLLIDKALTIDAPNKRRITLKGNAAFRIIEA
                     RGILSIDGLIIRDGAAADGDGGGIYVRPPSSAVLNLTNVAILNNTAQRGGGIYMNNGT
                     LYLFDSAVAGNTANAANGAGGVDVFRTTTRITNSTFSGNQSTSTESGVGAIKITDSQI
                     WQIIHSTIVYNSSNGSQATGSVGGLLAQNGAAGPFWNTIIARNTGLNPDFYGRAGGSH
                     SLVGVVPENSGMIDGVNGNLVGTATAPLNPQIGVLNDNGGGLPTHALLSSSPAIDAGN
                     NDLSFDRHGDPLTVDQRGYHRIINSTVDMGAYEFNAQPLLKPSLVTGQVRGANGRGIS
                     GAIVQLRGESGETRFTVTNPFGFYRFVNVAASASSYTVECLNKRRAFPAQNVLLEEVV
                     EYINFRAN"
     gene            complement(1962..>3094)
                     /locus_tag="VGB68_17320"
     CDS             complement(1962..>3094)
                     /locus_tag="VGB68_17320"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY0051057.1"
                     /translation="GLARYTSTGQLDSSFGNGGKVITETQNVSALRQIAIQSDGKIIA
                     VGSTTVSDFAVVRYTTGGALDTTFGGGDGIAITDFYNEADNATSVKLQSDGKIVVAGG
                     AVEPDFLTTLFAVARYNTNGSLDTSFNGNGLKTVFTECGPDALGCGAETMGIQPDGKI
                     AVAAGNDDIYLARFNADGSQINGVITTNIGGSSDKAYSMAIDSAGAILIAGSTLAKGD
                     ENFFVAKYLSSGVLDQSFDNGDGIVITPLGSNSDIGHAIAVQPNAGIIVGGSKGDFGA
                     KEFALVRYLGVQPTAANALITGRIVSANGSGISNVRVALTNGQGTTVYAITSSFGYYS
                     FYDIPVGQTYIITPNSTRYTFTPSSLVITLMDDLTTANFTAQ"
BASE COUNT          746 a          787 c          828 g          733 t
ORIGIN      
        1 ggcggcggta atcaagccgt gaagaattcc cgcgacggct ccgagcataa aagatttttg
       61 ccacggatta tcaaacgaac cgataggctc catctggatg ccgccggata acagaaagag
      121 cgagagactg cagcaaatta tagaactgac tgaaatatta aggagaaccg gtaagaaaac
      181 ttgaaaaaat gatgacatat cggattccat tttcgcagct tgaattaaaa tttaagaaga
      241 cttgaaatgc tgaagctcgc cggctttcag catcgtgatt tcggcggctg aagactccgc
      301 tttttgctac aaacaatttt aacaaaagaa tgatttgttt gtatataaat taaattgccg
      361 gtcgagtgga atcagcctgt ctgccgtcgt ttttcgaggc tcgaatcgag gcgcgcggtc
      421 agacagggca gggaactatt cgttttcaga agctagtttg ctcgaaaatt gatatattcg
      481 acgacttctt ccagcaaaac attctgcgcc gggaaagcgc gacgcttgtt taggcattcg
      541 acggtgtagg acgacgcgct cgcggcgaca ttgacaaaac ggtaaaagcc gaaaggattc
      601 gtcacggtaa atctggtttc accgctttcg ccgcgcaatt ggacgatcgc tccggaaata
      661 ccgcgaccgt ttgcgcccct gacctgcccg gtgacgagag aaggtttcaa cagcggctgc
      721 gcattaaatt cgtaagcgcc catatcgacg gtcgagttta taattctatg gtagccgcgc
      781 tggtcgacgg tcaaagggtc gccgtgacgg tcgaatgaaa ggtcgttgtt gcccgcgtcg
      841 attgccgggc tggaagataa aagcgcgtgg gtcggcaaac cgccgccgtt atcgtttaag
      901 actccgattt gcggattaag cggagcagtc gccgtcccga ccagattacc gttgactccg
      961 tcaatcatac cgctgttttc aggaacaact ccgaccaaac tgtgactgcc gccggcgcga
     1021 ccgtaaaagt cgggattaag tcccgtattc cgagcgataa tcgtattcca gaacggaccg
     1081 gcagccccgt tctgagccag taaaccgccg acgctgcccg tcgcctgcga gccgtttgaa
     1141 gaattataaa caatcgtgct gtggataatc tgccagattt gcgaatcggt tattttaatc
     1201 gcgccgacgc cggattcggt cgaagtggat tgattgccgg aaaaagtgct gtttgtaatt
     1261 cgcgtcgtcg tgcggaaaac atccactccg cccgcgccgt tagccgcgtt tgccgtattg
     1321 ccggcaacgg cgctgtcaaa caggtagaga gttccgttgt tcatgtaaat tccgccgccg
     1381 cgctgcgccg tattgttgag aatggcaacg tttgtcaggt ttagaactgc gctgctcggc
     1441 ggtctgacat aaattccgcc gccgtcgccg tccgccgccg cgccgtcgcg gataatgaga
     1501 ccgtcgatag agagtattcc ccgcgcctcg ataattctga aagcggcgtt gcctttaagc
     1561 gtaatgcggc gcttgttcgg cgcgtcgatg gtcaacgctt tatcaatcaa aagagttctg
     1621 gttaattgga ttgttccgcc gcgcaaaaca cggtcaaaaa taatcgtgtc gccggcgacc
     1681 gcgaatttga cggcttcgcg caggctgcaa tgcgcatcgc acgccgcatc atcgctatca
     1741 tccagagtgc tgacagtcag gacggcagcc cgtaaattaa cagtcaaaaa gagaacggta
     1801 aaggctatga agatgagtcg taatttcatt ttttcgggtg aaagcaagtg ttgacgacaa
     1861 agtgcaaaag gaaagaatat tagtataatt atttgtcttt ccttttgcaa attgccgcat
     1921 aagtgccgct ctgaggcttg agccgccaag cttccgcttg attactgggc ggtaaaattc
     1981 gcggtcgtca aatcatccat taaggtaatc acgagcgagg acggagtaaa agtataccgc
     2041 gtcgaattcg gggtgatgat ataggtttga ccgaccggaa tatcgtaaaa gctgtaataa
     2101 ccgaacgagc tcgtgatggc gtaaacagtc gttccctgac cattggtcaa ggcaactctc
     2161 acgttggaga ttccgcttcc gtttgccgaa acgatacgtc cggtaattaa ggcgtttgcc
     2221 gccgtcggct gaactcccaa atatctgacc agcgcgaatt ctttggcgcc gaaatcgcct
     2281 tttgagccgc cgacgatgat tcccgcgttc ggctggacgg cgatggcgtg accgatgtcg
     2341 ctgtttgagc cgagcggcgt aatgacgatg ccgtcgccgt tatcaaacga ctggtcgagc
     2401 acgccgctgc tcagatattt tgccacgaag aaattctcgt cgcctttggc gagggttgag
     2461 ccggcgatga gaattgcgcc cgccgaatca atcgccatcg aatatgcttt atcggagctg
     2521 ccgccgatgt tggtggtaat taccccgttt atctgagacc cgtcggcgtt aaagcgcgcc
     2581 aggtaaatat cgtcgttgcc ggcggcaacc gcaatcttcc cgtcaggctg aatgcccatc
     2641 gtttccgcgc cgcaccccaa agcatccggt ccgcactcgg taaaaactgt ttttaagccg
     2701 tttccattaa aactggtgtc gagtgacccg ttcgtattgt atctggcgac ggcaaacagc
     2761 gtggttaaga aatccggctc gaccgctccg ccggcaacca cgattttccc gtcggattgt
     2821 aatttaaccg acgtggcatt gtctgcttca ttataaaaat cggtaatggc aatgccgtcg
     2881 ccgccgccga aagtggtgtc taatgcgccg ccggtcgtat aacgcacaac ggcaaagtcg
     2941 ctgacggtcg ttgagccgac ggctataatt ttcccgtcgc tctggatagc aatctggcgc
     3001 agggcggaga cattctgggt ctcagtgata acttttccgc cattaccgaa agacgaatca
     3061 agttgtccgg tcgaagtata tctggctaac ccga
//
LOCUS       DASWNE010000884         6077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_522808, whole genome shotgun sequence.
ACCESSION   DASWNE010000884 DASWNE010000000
VERSION     DASWNE010000884.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522808"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="VGB68_17325"
     CDS             <1..833
                     /locus_tag="VGB68_17325"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0051058.1"
                     /translation="KLVFRKFREFFGGSLRSCITGGAALSDDIYYIFNGAGVSIMQGY
                     GLTETSPVISTNNPMSSRVGSVGKPLRNNEVRIAADGEIEASGPGVMLGYYNKPQATR
                     EAFTEDGWFKTGDIGRIDADGFLIITDRKKELFKTSGGKYIAPAPIEQMIKASRFVNQ
                     VVLVGNERNFPAALIVPNFESLAHYVKLKGIDAGSHAEMCRHPRIVNLLERQIAELTK
                     DLSQFEKVKKIALLENELSVEGGELTPTLKVKRRVVNEKYRDVIEKIYTQSEKEKPVS
                     "
     gene            complement(875..1855)
                     /locus_tag="VGB68_17330"
     CDS             complement(875..1855)
                     /locus_tag="VGB68_17330"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HEY0051059.1"
                     /translation="MKRNILWIFAITLCLAQIAFAQEKVSTQTVSQVAPRGYLVGPGD
                     VIEGKVLGEKDFDFTATIDEDGNFEVPFFEKPVPAVCRTEKELRSEVTRLVSKYVRNP
                     QVSVQVRERKSRPPATIYGEVRQQQQVILTRRARLLELLSSAGGVTEKAGGMIQIFRT
                     QPLLCADPNEVNDWKTEAGNGLDVPSRVYSLKSVRQGKDESNPVIYPGDIIVVQKAAP
                     VYIVGEILRPGEMTIPEGGLPLMQAIAMASGISREAKTKNIKIYRRKEGSTQPEVIMV
                     NYDQIKKGQQKDVILEPFDIVEVDKAKKKFTDYLIDFAIGIPNRLPIIPL"
     gene            2034..4019
                     /locus_tag="VGB68_17335"
     CDS             2034..4019
                     /locus_tag="VGB68_17335"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HEY0051060.1"
                     /translation="MKDSSLDKNQTEERFWFLRRPFQFLADLCVLSGAFFLAYLLRFD
                     FRLSEYYLENALNQLPFVILVQFSSLFLIGAYSILWRYVSLEDIKAFLKAAFISGVVL
                     IAFRLLLSPERFIRWQVPLSIILMDTMFAFAGLLALRVLRRFAYEFSEKRIVTGSRRR
                     LKPKSTLFVGAGRIAAAAVKEVAGRADAELQVKGFVDDDRRKKGGSVSGIKVLGTTDD
                     LARLADELSIEQVVIAIDHAQGKEIRRILDICREIPVKAQIVPSLNEIVHGRVNVSRI
                     RDVQIEDLLGRDPVQLDDKNLHEFLTGKTVMVTGAGGSIGSELVRQVANFLPAQLLLV
                     ERTEFALFQIERELVKDFTDLKFLPLLADVSDAPRMREIFEKFKPQVVFHAAAHKHVP
                     LMETNPTEAIKNNVFATKQIAELAGEFGANAFVLISTDKAVNPTSIMGASKRVAELVV
                     QSLRRRYDKTRYMAVRFGNVLGSAGSVVPIFREQILKGEPITVTDPKMTRYFMTIPEA
                     SQLVLQAGALGAGGEIFILDMGEPVKILDLAEDMIRLSGLQPYEDIDIIFTGIRQGEK
                     LFEELEITGENLLKTQHPKIFIGKIAEFSAEEVTQILSNFETAVNESSETKIRALLNH
                     FLPEAKINEPKIPENTEINPKEEKFFTKPPQLGLVEK"
     gene            4064..4495
                     /gene="nikR"
                     /locus_tag="VGB68_17340"
     CDS             4064..4495
                     /gene="nikR"
                     /locus_tag="VGB68_17340"
                     /inference="COORDINATES: protein motif:HMM:NF002169.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HEY0051061.1"
                     /translation="MSETIRFGVSIDQDLLENYDRLIAERGYATRSEALRDLIREALI
                     QQKIETQSEIHALGSLTLVYDHHASHLLQEMAQLQHNFHQLILSVMHLHVSHDDCMEI
                     IALRGVAAEIAELANGLLSLKGIKNGKLFLTLPSSGITNSK"
     gene            4651..>6077
                     /locus_tag="VGB68_17345"
     CDS             4651..>6077
                     /locus_tag="VGB68_17345"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY0051062.1"
                     /translation="MKNRIFLRHFFTFLSIIALGVIFNVHLFAQSERTFSGVVLTQQF
                     ELVPNVSIEVRTSDGKLETTTDAEGKFSLKVPSEPISAKFSGRNLQTVTRIFAVTEAL
                     ENLQIKITYIVPPVAENVVIEAGALTPEVERRNDTIYKNTLFGRDDQLIFTLNAGINA
                     GQHEGGGKSLEIRRFGFNTDHGGVNGGVKILVDNIQQNQGTQGHGQGYLGNLKSLSPE
                     LVQDVSIVNGPFSAAYGDFSGLGVVRIRLKESLPQRFTARVQAGSFNTARGFFAYSPQ
                     WKNIDAFLAYEPAYTDGPFENRLRYRRDNVSGNFTYKFSEAQALGFKLNIGRSDFTSS
                     GQIPLDSAARGELDRFGFIDPDNGGKVRLGTIGTYYRREWKSGATLKADAFVSRSLFD
                     LWSNFTFFLNDPIYGDEIQQHDSRLQEGANVQYLQPYKLFGRQSLLTVGGNLHFNQIN
                     VGLYPSAGRNPNRKFLPENIDNPNVL"
BASE COUNT         1468 a         1546 c         1567 g         1496 t
ORIGIN      
        1 ataaattggt tttcagaaaa tttcgcgaat ttttcggcgg cagcctgcgt tcgtgcatca
       61 cgggcggcgc ggctctgtcc gacgatattt attacatttt caacggcgcg ggcgtctcga
      121 ttatgcaggg ctacggcttg accgaaacct cgcccgttat ctcgaccaac aacccgatgt
      181 cgagccgcgt cggcagcgtc ggcaagccgc ttcgcaacaa cgaggtgcgc atcgccgccg
      241 acggcgaaat cgaggcgtca ggtccgggcg tgatgctcgg ctattacaac aagccgcagg
      301 cgacgcgcga ggcgtttacc gaagacggct ggtttaaaac cggcgacatc ggcagaatcg
      361 acgccgacgg ttttttgata atcaccgacc gcaaaaaaga gcttttcaag acttccggcg
      421 gcaaatacat cgcgcccgcg ccgatcgagc agatgataaa agcctcgcgc tttgtcaatc
      481 aggtcgtttt agtcgggaac gagcgcaact ttccggcggc gttaatcgtg ccgaatttcg
      541 aatcgctcgc gcattacgtg aagctgaaag gcatcgacgc cggctctcac gccgaaatgt
      601 gccgtcatcc gcgcatcgtc aatcttttgg aaagacagat tgccgagctg accaaagatt
      661 tatcgcagtt tgaaaaggta aaaaaaatcg ctttgctgga aaacgaattg tcggtcgaag
      721 gcggtgaatt gacgccgaca ttgaaagtca agcgccgcgt cgtgaacgaa aaataccgcg
      781 acgttatcga aaaaatctac acgcaatccg aaaaagaaaa gcctgtgagt taatgattct
      841 cacaggcgcg aataaatatt tttgcaaatt tcgtttagag aggtattatc ggcagccggt
      901 tcggaatgcc gatcgcaaaa tcaatcagat aatcggtaaa ctttttcttg gctttatcga
      961 cctcgacaat atcgaaaggc tcaagaatca cgtccttttg ctgtcccttt ttgatttggt
     1021 cataattgac cataataact tccggctggg tcgagccttc tttacgacga taaattttaa
     1081 tatttttggt tttcgcctcg cgcgatattc cgctcgccat cgcaatcgcc tgcattaaag
     1141 gcagacctcc ttcgggaatc gtcatttcgc ccggtcgcag aatttcgccg acgatataaa
     1201 ccggcgcggc tttctgcact acgataatat cgcccggata gattaccgga ttcgactcgt
     1261 ccttgccttg tctgacactt ttgaggctgt atactcgcga aggcacgtcc aaaccgttac
     1321 cggcttccgt tttccaatcg tttacttcgt tcgggtcggc gcacaataaa ggctgggtgc
     1381 ggaaaatctg aatcataccg ccggcttttt ccgttacgcc gccggcagaa gaaagaagct
     1441 ccagcagacg cgcgcgacgc gttaagataa cctgctgctg ctggcgaact tcgccgtaga
     1501 tggtcgcggg cgggcggctt ttgcgttccc tgacctgcac gctgacctgc gggttgcgca
     1561 catatttcga aacgagcctg gttacttccg agcgcagttc cttttcggtg cggcaaactg
     1621 ccggaacggg tttttcgaaa aacggaactt caaaattgcc gtcttcgtca atggtggcgg
     1681 taaaatcgaa atctttttcg cccagaactt ttccttcgat aacgtcgccc ggtccgacta
     1741 aataaccgcg cggggcaacc tgcgaaaccg tctgcgtcga aaccttttcc tgcgcgaaag
     1801 cgatttgagc aaggcaaagc gttatcgcaa aaatccacaa aatatttctt ttcatcgtgt
     1861 taaaacctct ccgggatgtg tggcagctaa taagagtatc ctagaaaagc tttcgattca
     1921 acttcccacg ccttgagttg tctgccgcgt cagaaacatt taatgttata aaaactattt
     1981 gacaaggcaa tgttttgccg agtaacttca ttttagagaa gtttgttcag ccgatgaagg
     2041 attcttcctt agataaaaat caaaccgaag agcgtttctg gtttctgcga cgtccgtttc
     2101 agtttctggc ggatttgtgc gttttgagcg gagcgttttt tctggcgtat ctgcttcgtt
     2161 ttgatttccg gctttccgaa tattatctgg aaaacgcgct gaatcagctt ccttttgtga
     2221 ttctggttca gttttcttcg cttttcttaa tcggcgcgta ttcgattttg tggcgctacg
     2281 tcagcctcga agacattaaa gcttttctga aagccgcttt tatttccggc gtcgttttga
     2341 ttgcctttcg ccttttgctt tcgcccgagc gttttattcg ctggcaggtt ccgctttcca
     2401 ttattttgat ggacacgatg tttgcgtttg ccggacttct ggctctgcgc gtgctgcgaa
     2461 gatttgctta cgagttttcc gaaaagcgta tcgtgacggg cagccgtcgg cgtctgaaac
     2521 cgaaatcgac gctttttgtc ggcgcgggca gaatcgcggc ggcggcggtc aaggaagtgg
     2581 cgggtcgcgc cgacgccgag cttcaggtga aaggttttgt cgacgacgac cggcgtaaaa
     2641 aaggcggcag cgtcagcggc atcaaggttc tgggaacaac cgacgatttg gcgcggttgg
     2701 ctgacgaact gagcatcgag caggtcgtca ttgcgattga ccacgcgcag ggcaaggaaa
     2761 ttcggcgcat tctcgacatc tgccgcgaga ttcccgtcaa agcgcaaatc gtgccgagcc
     2821 tgaatgaaat cgttcacggt cgcgtcaatg tcagccgcat ccgcgacgtg cagattgaag
     2881 accttttagg acgcgacccc gttcagctgg acgacaaaaa cctgcacgaa tttctgaccg
     2941 gcaaaaccgt aatggtgacg ggcgcgggcg gctcaatcgg ctcggagctc gtccgtcagg
     3001 tcgccaattt tctgcccgcg cagcttcttc ttgtcgagcg aacggagttt gcgctgtttc
     3061 aaatcgagcg cgaactggtc aaagatttca cggatttaaa attcctgccg cttctcgccg
     3121 acgtttcgga cgcgccgcga atgcgcgaga ttttcgagaa attcaagccg caagtcgttt
     3181 tccacgccgc cgcgcacaag cacgttccgc tgatggagac gaacccgacc gaagcgataa
     3241 aaaacaatgt ctttgcgacc aaacaaatcg ccgagctggc gggcgaattc ggcgccaacg
     3301 cctttgtttt aatttcgacc gataaagccg tcaatccgac ttcgattatg ggcgcgtcaa
     3361 aacgcgtcgc cgagctcgtc gtgcaaagcc tgcggcgcag gtatgacaag acgcgatata
     3421 tggctgtccg tttcggcaac gtcctgggtt cagccggttc ggtcgtgccg atttttcgcg
     3481 agcaaatcct gaaaggcgag ccgataaccg tcaccgaccc gaaaatgacg cgttatttta
     3541 tgacgattcc cgaagcctcg cagcttgttc tgcaagccgg cgcgctcggc gcgggcggcg
     3601 agatttttat tctcgatatg ggcgagccgg tgaaaattct ggatttggcg gaagatatga
     3661 ttcggctttc cggcttgcag ccctacgaag acatcgacat tatttttacg ggcatccggc
     3721 agggcgaaaa gctgtttgaa gaactggaaa tcacgggcga aaatttattg aaaacacagc
     3781 atccgaaaat ttttatcggc aaaatcgcgg aattctcggc ggaagaagtg acgcagattc
     3841 tgtcgaattt tgaaaccgcc gtcaacgaat cgagcgaaac gaaaatccgc gctttgctga
     3901 accatttcct gcccgaagcg aaaatcaacg aacctaaaat accggaaaat accgaaatca
     3961 atccgaagga agaaaagttt tttaccaagc cgccgcaatt aggtctggta gagaaataac
     4021 cggatttccg ggcagttagg gaaacaggca aggagcagaa tcattgagcg aaacaatcag
     4081 attcggagtg agcatcgacc aggatttgct ggaaaattat gaccgcctga tcgccgagcg
     4141 cggttacgcg actcgttccg aagccctgcg cgatttgatt cgcgaagcct tgattcaaca
     4201 gaaaatcgaa acgcagagcg agattcacgc gctgggcagc ttaacgctcg tttacgacca
     4261 tcacgcgagt catctgctcc aggaaatggc gcagctccag cacaatttcc atcaattgat
     4321 tttatccgtg atgcatctcc acgtcagcca cgacgattgt atggaaatca tcgccctgcg
     4381 cggcgtggca gcggaaatcg ccgagctggc aaacggtctt ctgagcctga aaggcatcaa
     4441 aaacggcaag ctgtttctga ccctgccgtc gtcgggcatt acgaattcga aatagtttaa
     4501 gataattcaa aataatttga aaccgattgt ttaaattgag cgaatgtata aatatcaaag
     4561 agtgttacta attttgcttt cgtgttacga cgattggtca aattgatttt aagcggcatc
     4621 gggatttcaa ttgatttcgg atttatcttt atgaaaaaca gaatctttct tcggcatttt
     4681 tttacttttc tttcaataat cgcgctcggc gttattttca atgttcatct gttcgcgcag
     4741 agcgaaagaa ctttttcggg cgttgttttg acgcagcaat tcgagcttgt tccgaacgtc
     4801 tcgatcgaag tccgcacatc ggacggaaaa ctggaaacga cgaccgacgc ggaagggaaa
     4861 ttttctctga aagttccgtc cgagccgatt tccgcaaaat tttccggcag aaatctgcaa
     4921 accgtgacgc gaattttcgc cgtcacggag gcgctggaaa atctacaaat taaaatcacg
     4981 tatatcgttc cgccggtcgc cgaaaacgtc gttatcgagg cgggcgcgtt gacgccggaa
     5041 gtcgaacggc gcaacgacac gatttataaa aacacgcttt tcgggcgcga cgaccagttg
     5101 attttcactc tgaacgcggg aatcaatgcc ggacagcacg aaggcggcgg gaaatcgctg
     5161 gaaatccggc gtttcggttt caacacagac cacggcggcg tcaacggcgg cgtcaagatt
     5221 ctggtcgata acatccagca gaatcaggga acgcagggac acgggcaagg ctatctggga
     5281 aatctgaaat cgctctcgcc cgaactcgtg caggacgtct ccatcgtcaa cggacctttt
     5341 tccgccgctt acggcgattt ttccggcttg ggcgtcgtcc ggattcgttt gaaagaatct
     5401 ctgccgcagc gctttacggc acgcgttcag gcgggaagct tcaacaccgc gcgcggattt
     5461 ttcgcctaca gcccgcagtg gaaaaacatc gacgcctttc tggcgtatga gcccgcttac
     5521 accgacggac ctttcgaaaa tcggcttcgc tatcgacgcg acaatgtttc gggaaatttc
     5581 acatacaaat tcagcgaggc gcaggcgctc ggctttaaac tgaatatcgg gcgcagcgat
     5641 tttacttctt cgggacaaat tcctctggat tcggcggcgc gcggcgagct cgaccgtttc
     5701 ggttttatcg acccggacaa cggcggaaaa gttcgtctgg gaacaatcgg gacgtattat
     5761 cgacgggaat ggaaatcggg cgcgacgctt aaagccgacg cttttgtttc gcgctcgctt
     5821 ttcgatttat ggtcgaattt tacgtttttt ctaaacgacc cgatttacgg cgacgaaatc
     5881 cagcagcacg attcgcgttt gcaggaaggc gcgaacgttc aatatcttca gccgtataaa
     5941 ttgttcggca ggcaatcgct tttaaccgtc ggcggaaatc tgcatttcaa tcaaatcaac
     6001 gtcggtttgt atccttccgc cgggcgcaat ccgaaccgca agtttttgcc ggaaaacatc
     6061 gataatccga acgtgct
//
LOCUS       DASWNE010000885         3145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_207362, whole genome shotgun sequence.
ACCESSION   DASWNE010000885 DASWNE010000000
VERSION     DASWNE010000885.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207362"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VGB68_17350"
     CDS             complement(<1..589)
                     /locus_tag="VGB68_17350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051063.1"
                     /translation="MLKRFVVFLLAVFALCGATWAQSGRKVQPTPTPAPVVEEESSYS
                     ESAPSKTRRIYPRNWRSDRDKSKDKPAPKTRTQPTPNTQNAPNAQTSAPTADATIDEQ
                     DVIKVETNLISIPVSVFDRNGLYIPNLRQSDFKIFEDGKEQEVAYFGTSEKPFTVILL
                     IDTSPSTQYKIEEIQTAATAFVDQLKPQDNVMVIEF"
     gene            987..2144
                     /locus_tag="VGB68_17355"
     CDS             987..2144
                     /locus_tag="VGB68_17355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051064.1"
                     /translation="MRYFSLIVLLLVLSVSCLGQEVKPEGKSKPKLRTTPAKPRAAKA
                     KSASKPEPQTKAGQSKPAEIKPQDKPKAETTPAKSDSTKARPADKEKSQASDSKPNPT
                     QDESKGKPAAQTKAAPSKQTKAGSKDKPKPDTAKQSGATKAAIKAKPKTIKAETGKAK
                     RVKVESKDKSKQTAVEPKEKQKTLTPNPPTQTAPVKSKADAVSVKPKADDVPVKPKTD
                     AVSVKPKPAPKTGFQPCDLKDAPAIRSLRLGMSRREVDGIIPTAGRVKYINSSTITAY
                     PDAASGFENIGQVTAQFDEDRLNVLEIDYDRSAVQWKSVEEFAGNLSDNLKLPRNAWD
                     FRFKKWNRAEMKCKGFSLKINSEMNELTLENSDSPAASGQEASGNKKSFKP"
     gene            complement(2318..2512)
                     /locus_tag="VGB68_17360"
     CDS             complement(2318..2512)
                     /locus_tag="VGB68_17360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051065.1"
                     /translation="MDENVTIVFHGFLNLMTKEKLQLINEINNYFDSNDRDPIRRDNE
                     VKFKNIDFSSPAKSCKCCGK"
BASE COUNT          935 a          742 c          700 g          768 t
ORIGIN      
        1 caaactcgat aaccatcacg ttgtcctgcg gttttaactg gtcgacgaaa gccgtcgcag
       61 cggtttgaat ttcttcgatt ttatattgcg tcgaagggct ggtatcgata agcagaatca
      121 ccgtgaaagg cttttcggaa gtgccgaaat aagcgacttc ctgttccttg ccgtcttcaa
      181 aaatcttgaa atccgattgg cgcaggttcg ggatataaag cccgttgcgg tcaaaaaccg
      241 agacgggaat cgaaatcaga ttggtttcga ccttaattac gtcctgctcg tcaatagtcg
      301 cgtcggctgt cggcgcgctc gtttgggcgt tcggcgcatt ttgagtgttc ggcgtcggtt
      361 gagttcgcgt tttgggcgcg ggtttgtctt tcgatttatc acggtcgctc cgccagtttc
      421 gcgggtaaat ccggcgagtt ttcgagggcg cggattccga gtagctcgat tcttcttcga
      481 cgacgggcgc gggcgtcggc gtcggctgaa cttttctgcc cgactgcgcc caggttgcgc
      541 cgcacaaagc aaaaaccgcg agtaaaaaaa caacgaatct cttcagcata atttcctctt
      601 taatcaaacg ggtaatcagc cgctttgatg cagaaaatgt caaaaaggtt agcaatttat
      661 ctgctcgaac aaagtaaaga attgtaaaaa aatcgaaaat gaacagcgga aaatgcaaaa
      721 taaaattatt tttttcgttt gcgctttgca tttttaattt tgcattaatt acaattctac
      781 caaaatcatt ctgagaggaa tcaaaaaatg caacccaatg ccgccgcctt atcaatgata
      841 agttaacctt ttcgtccgct ataagacata gccggaaagt tttgatgttt atttataatg
      901 catttatcaa tgtgttcgcg cttttttcgc tttccatacg gataaaatat tgacgaaaga
      961 aaagaaaaag tcttatcatc tttggcatga gatattttag cttgattgtg ctcctgctgg
     1021 tgctttccgt ctcctgtctc ggtcaggaag ttaaaccgga aggtaaatcc aagcccaaat
     1081 tgcggacgac gcccgccaaa ccgagggcgg cgaaagctaa atccgcgtcc aagccggagc
     1141 cgcagacgaa agccgggcaa tccaagccgg ctgagataaa accgcaggac aagccgaagg
     1201 cggaaaccac gcccgccaaa tccgattcca ccaaagcccg accggcggac aaggaaaaat
     1261 cccaggcgtc ggattcaaaa ccgaatccga cgcaggacga gtcgaaaggc aagcccgccg
     1321 cgcagacgaa agccgcgccg tcaaaacaga cgaaagccgg ttcgaaagat aaaccgaaac
     1381 ccgatacggc gaaacaatcc ggcgcgacca aagccgcgat aaaggctaag cccaaaacga
     1441 ttaaagccga aaccggcaaa gcaaaacggg tgaaagtcga atcgaaagat aaatcaaaac
     1501 aaacggcggt cgagccgaaa gagaagcaga aaacgctgac gccgaatccg ccgacccaga
     1561 cggctccggt caaatcgaaa gccgacgccg tttccgttaa gcccaaagcc gacgatgttc
     1621 cggttaaacc gaagaccgac gccgtttcag tcaaaccgaa accggctccg aaaaccggat
     1681 ttcaaccgtg cgatttgaag gacgcgcccg ccattcgcag cctgcgactg ggaatgtcga
     1741 gaagagaggt cgacgggata ataccgaccg cagggcgagt taaatacatc aattcttcga
     1801 ccattaccgc ttacccggac gcggcgagcg gttttgaaaa catcggtcag gtcacggcgc
     1861 agtttgacga agaccggctc aacgttctgg aaatcgacta cgaccgcagc gccgtccagt
     1921 ggaaaagcgt cgaggaattt gccggaaatc tttccgacaa tctcaaactg ccgcgtaacg
     1981 cctgggattt ccgtttcaaa aaatggaacc gcgccgaaat gaagtgcaag ggattttcct
     2041 taaaaataaa ttcggagatg aacgagctga cgctggaaaa ttctgattcg cccgccgcct
     2101 cggggcagga agccagcggc aataagaaaa gttttaagcc ttaaacggct ttatcttcga
     2161 taagccggaa acacgctcgg taaaggagcg tgctgattta gtgaaaaacg aaaagtgaaa
     2221 ggcgttctca ttttcactat tcacttttcg tttttcgttg aatgaccgtt tccgcctttc
     2281 ttgaatcaaa ccgaaccgga atcaaactgc attcgctcta tttcccgcag catttacagc
     2341 ttttcgcggg cgagctaaaa tctatatttt taaatttcac ctcgttgtcg cgccttatcg
     2401 ggtcgcggtc gttcgaatcg aaataattgt taatctcatt aattaattgc agcttttctt
     2461 tagtcatcag atttaaaaac ccgtgaaaaa cgattgtcac attttcatcc ataattttat
     2521 ttccttaaat ttatgcggct ccatattccc gcatgaccgc ataaaccgcc agagccggca
     2581 gagccgcttg caaaggcgcg tgaatcggct ttgcacaaaa ataaagaaag ggaaaattgt
     2641 aaattctagg ctttgcacca aaactccatt ttgcaccaaa agtgcttaag tctgcaccaa
     2701 tactattgca aagatgtagc taaaagcgtt atgaaaaaaa taatgcctga caacaaaaaa
     2761 tattgcttga catctaagga aaattaaatc attatgtaga aaaatttttt ttaaaagaga
     2821 tataggcagg cttgccgtag atatagactt tttccaaaac agaaaccagc gcattaatcg
     2881 cgctaattga cgcgcagatt acgccaatta aagctactat ttccagaaaa ctccatttca
     2941 taaccgcatt atcttacttt gagaatagag agtcaacaaa aatttttgta aataaaagga
     3001 gagacatttc acctctcctt ttattattta tgtgctttgc acaacttcaa caacatcatt
     3061 aatgggaaaa cctacttgta actgtggttc tattccttta cttaacgcat tatcgtattc
     3121 ttctttatta gttacgatga cacta
//
LOCUS       DASWNE010000886         2676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_380956, whole genome shotgun sequence.
ACCESSION   DASWNE010000886 DASWNE010000000
VERSION     DASWNE010000886.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380956"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VGB68_17365"
     CDS             <1..369
                     /locus_tag="VGB68_17365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051066.1"
                     /translation="FAVVFVLLQFFIEPSARHETTKDLIVTANSQGFADARILTLHNT
                     SHNAEFYGAGRLQRLANGKQRYLYGVAEVKEFIEQNENKPVLVLVPLEYLKDLTESDL
                     VSSRVLKDNGKLAIAVVNLR"
     gene            539..1345
                     /gene="trpA"
                     /locus_tag="VGB68_17370"
     CDS             539..1345
                     /gene="trpA"
                     /locus_tag="VGB68_17370"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEY0051067.1"
                     /translation="MEKSRIAESFDRLKNENRKGFIPFITAGDPDLKRTKDLILMLAR
                     EQATVIELGVPFSDPMADGVVIQRASERALKHDFGIAEILDLVREARAETNAAIILFS
                     YFNPLLQYGLEKLCREARAAGVDGFLVTDLVPEEAAEFSRLLRQNNLDMIFLVAPTSS
                     DERLKMVAERAGGFIYAVSRAGVTGAREQTSQSAEVLVERVRKFSDLPVAVGFGISNA
                     GQIAETWKYADAAVVGSAIVREIENSSGEDGLVEKIGEFVRKLLPESPSN"
     gene            1653..2306
                     /locus_tag="VGB68_17375"
     CDS             1653..2306
                     /locus_tag="VGB68_17375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051068.1"
                     /translation="MTEKQNIIEKRVNELNRRGQKIDAVLDKIKQTDSQPLQEVRVKL
                     LSAREVVAGQFARYELQKQKIELVRLQNAVSPYLFQMARLDEIEMENGLAAIERTARE
                     INHIRLNLTRYDAIDFPKRTLPEKENFLSQLAETESSCEKLREALLAKQAAHALAGIH
                     PLEETLNLPNTKDLAHAAETFNLQTTLTDFSESFDQLEREYKRLKAEDEVSRKFLDE"
     gene            2310..2609
                     /locus_tag="VGB68_17380"
     CDS             2310..2609
                     /locus_tag="VGB68_17380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051069.1"
                     /translation="MAKIETGEMVVLVMHSPREKIFGVLHEINASGAFVRGIDLNAFD
                     DWTRAIANDEPFFGMNDYFFPMWRVERMTRDERSGEIPSMAEQFEQRTGLKISEF"
BASE COUNT          684 a          630 c          703 g          659 t
ORIGIN      
        1 tttgctgtcg tttttgtgct tttgcagttt ttcatcgagc cttccgcgcg gcacgaaaca
       61 accaaagatt taatcgtcac ggcaaattcg caaggatttg ccgatgccag aattctgact
      121 ttgcataaca cttcgcacaa cgccgaattt tacggcgcgg gcagattgca gcgattggct
      181 aacggaaaac agcgttatct ttacggcgtc gcggaagtca aggaatttat cgaacaaaac
      241 gaaaacaagc cggttctggt tctggtgccg ctcgaatatt tgaaagattt aaccgaaagc
      301 gatttggttt cgagcagggt tttgaaagac aatggcaagc tggcaatcgc ggtggtaaat
      361 ttaagataat tcaactgccg gcaaaaaagc cgaagaaaaa cgagaccgca gacggacgcg
      421 gacaaacgcg gataatttaa ccgggcagaa tcggtttcgc ggcttgattt atcgaattga
      481 atcttttttc tatctgcgct catctgcgtt taattttttt ctgtgctgaa ataaatttat
      541 ggaaaaatcc cgaatagcgg aaagcttcga ccgcctgaaa aatgaaaatc gcaaaggttt
      601 tattccgttt ataacggcgg gcgacccgga tttgaaacgg acgaaagatt tgattttgat
      661 gctggcgcgc gagcaggcga ccgttatcga gcttggagtt ccgttcagcg acccgatggc
      721 tgacggcgtc gttattcagc gcgcttcgga acgcgcttta aaacacgatt tcggcatcgc
      781 ggaaattctg gatttggtgc gcgaggcgcg tgcggaaacg aacgcggcga taattctttt
      841 cagctatttc aatccgcttt tgcaatacgg tctggaaaag ctctgtcgcg aggcgcgcgc
      901 ggcgggcgtt gacggttttc tggtgacgga tttagtgccc gaagaagccg ccgagttttc
      961 tcgccttttg cggcaaaaca atctggatat gatttttctg gtcgcgccga cctcatcgga
     1021 cgagcggctg aaaatggtcg ccgagcgggc gggcggcttt atctacgccg tctcgcgcgc
     1081 gggcgtcacc ggcgcgcgcg agcagacgag ccaatccgcc gaagttttgg tcgaaagagt
     1141 gagaaagttt tcggatttgc ccgtcgccgt tggtttcggg atttctaacg ccggacaaat
     1201 cgccgaaacg tggaaatatg cagacgcggc ggtcgtcggc tcggcgatcg tgcgcgagat
     1261 tgaaaattcg agcggcgaag atggtttagt ggagaaaatc ggcgagtttg tgcgaaaatt
     1321 gttgcccgaa agcccttcga actgagaata tgctcgcaaa ctgcgcttac tgtaaatttt
     1381 atatccggct cgacgacgaa ttttgtttaa actgcgggat gtccgcgcgt gccgtcgata
     1441 ccgggaaacc ttatataaac tttctgactg tcggcggctt gctttgcgcg gcggcggctt
     1501 acctgattat tgatatatta gggaaaaacc tgttgccaca cactccgttt attgccaatt
     1561 tcatatttgt tttttgcctt ctgctgattc ccgcctttat cgtcttcgaa cttttttaca
     1621 acaaaaaatt aaacaaaaag cttctttcaa atttgactga aaaacagaac atcatcgaaa
     1681 aacgcgttaa tgaattgaac cggcgcgggc agaaaatcga cgccgtttta gataaaatca
     1741 aacaaaccga cagccagccg ctgcaggaag ttcgcgtcaa gcttctctcg gcgcgcgaag
     1801 tcgtcgccgg tcaatttgcg cgctacgagc tgcaaaagca gaaaatcgag cttgtccgcc
     1861 tgcaaaacgc cgtttcgcca tatcttttcc agatggcgcg cctggacgaa atcgagatgg
     1921 aaaacggctt ggcggcaatc gagcggacgg cgcgcgaaat caatcacata cggctgaatt
     1981 taacgcgcta cgacgcgatt gattttccga aaagaacttt gccggaaaag gaaaattttc
     2041 tctcgcagct tgccgaaacc gaatcttcgt gcgaaaaact gcgcgaggct ttgctggcaa
     2101 aacaggcggc gcacgccctt gccggcattc atccgctcga agaaaccctg aatctgccga
     2161 atacgaaaga tttggcgcac gcggctgaaa ctttcaacct tcagacgacg ctgaccgatt
     2221 tcagcgaatc gtttgaccag ctggagcgcg aatacaaacg cctcaaagcg gaagacgaag
     2281 tcagccgaaa atttttagac gagtaattta tggcaaaaat cgaaacaggt gaaatggtcg
     2341 ttctggtaat gcacagcccg cgcgaaaaaa tcttcggcgt gctgcacgaa atcaacgctt
     2401 cgggcgcgtt tgtccgcggc atcgatttga atgccttcga cgactggacg cgcgccatcg
     2461 ccaatgacga gccgtttttc ggaatgaacg attatttttt cccgatgtgg cgcgtcgagc
     2521 gaatgaccag agacgagcgc agcggcgaaa ttccgtcaat ggcggaacaa ttcgaacaga
     2581 gaaccggctt gaagatttcg gaattttaag atttttgctt tttaaaagcg tcggctttcg
     2641 ctttgagagc ttcgcttttt tgtctgaaat attttg
//
LOCUS       DASWNE010000887         7346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_340903, whole genome shotgun sequence.
ACCESSION   DASWNE010000887 DASWNE010000000
VERSION     DASWNE010000887.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7346
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340903"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1392
                     /locus_tag="VGB68_17385"
     CDS             <1..1392
                     /locus_tag="VGB68_17385"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEY0051070.1"
                     /translation="YSVVFGEDNYDDIANQFVWMEKHARDPKTGLLYHGWDESRRQKW
                     ANKETGLSPHVWGRAMGWYAIALVDVLDYFPKNHPRRGELVAILRREAEAITKYQDKD
                     GLWWDIIDLAGKDKNYHESSAAAMFVYALAKGVRQGNLPDKYLNTAQKGWAGIRKEFI
                     RERADGTLDWEGTVSVSGLGGNPYRDGSYDYYMSEKLRTNDAKGLGPAVMAAIEMENL
                     ERGRTGAGKTVLLDDYFNREVRKDGSVWHYKWNEKNHPGFYAWGKQFESYGAALDTLS
                     SAPTAANLKNADVYIIVDPDTEKETEKPNFVSRADAGTIAGWVKAGGVLVLMGNDFGN
                     SEFDNFNVLAKQFGIEFNRDSKYRVQNNNFAEGKITVAGSNPIFKTAKTLYLKELSTL
                     SVSTPAGAILEANGDKIMAAAKYGKGTVFAVGDPWLYNEYVDGRKLPAEYENLKAAND
                     LSAWLLARARKKK"
     gene            1407..1925
                     /locus_tag="VGB68_17390"
     CDS             1407..1925
                     /locus_tag="VGB68_17390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY0051071.1"
                     /translation="MIKNIFGIFVLTCALTLGSAAQTREPAAPIRPFVVLKKQSLGDL
                     QKKLSVDNKVEDLIGGAGMELRVAVQHDRTRESAEAESHDASDDVYYVLEGAAMLTLG
                     GKLENPRETSAGEWRSARIVGGERFEIKKGDLIIVPRGTPHHRVNAKGKQFSLILIKI
                     FADPLPAARARN"
     gene            2080..2847
                     /locus_tag="VGB68_17395"
     CDS             2080..2847
                     /locus_tag="VGB68_17395"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEY0051072.1"
                     /translation="MSDSKHDIFKTIEIEKGINTSEEVVSQLRNMIHSGALSAGDRLP
                     PERDLAKLLGVSRPTLRAGIRSLASVGILQSRQGAGTFVAQAEESPTLDSSPLRLMAS
                     LHGFTSDEMFEVRLSLEMSIAGLAAERATAEQMTQMAEEVAGMYASLDEPEQYLVHDM
                     RFHQIIAAASQNRILTALMNMVATILFEYRSKTVKRAKDLKESAEHHRNIYRAMRDRN
                     PEAARKAMRDHLVETQKAQRLEVEGNFPGENGHGKKS"
     gene            2953..4572
                     /locus_tag="VGB68_17400"
     CDS             2953..4572
                     /locus_tag="VGB68_17400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009038058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="HEY0051073.1"
                     /translation="MKRKILIVIIGLLSIFSPDTAAQPTKSKVWVADNGDGTYKNPIL
                     HADYSDPDAIRVGEDFYMTASSFNAAPGLPVLHSKDLINWRLINYVFTRQPPFEVFDK
                     PQHGGGVWAPSIRYHDGEFYIFYPDPDFGIYMTKTKNPAGEWSKPLLIKEAKGWIDPC
                     PLWDADGSAYLVSAFAGSRAGIKTVLIVSRMSADGTRLLDDGVMVFDGHDAHPTVEGP
                     KFYKRNGFYYIFAPAGGVPTGWQTVLRSKNIYGPYDEKIVLAQGSTQINGPHQGAWVD
                     TPTGEDWFLHFQDKGAYGRIVHLQPMKWVDDFPVMGIDKDGDGTGEPVLSFKKPNVGK
                     TFPVETPPESDEFNDAKLGLQWQWQANFQPTWAFTYPPKGFLKMFSVELPENYKNLWD
                     VPNLLLQKFPAEEFTVTTKATLTPRFEGEKFALVVAGLDYSYIGVTNKGGRLYVAQAT
                     ARDADKGNAEAENVPFLLNEKTFYLRVKVSKGAMCSFSFSADGKNFTNAGVPFKARQG
                     KWIGARVGLFFTRKGKFNDAGSADVDWFRIE"
     gene            4690..5679
                     /gene="fbp"
                     /locus_tag="VGB68_17405"
     CDS             4690..5679
                     /gene="fbp"
                     /locus_tag="VGB68_17405"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF006778.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEY0051074.1"
                     /translation="MITTLQQHVVQEQAAMSDSPDELQWIFSGLALATKMIQAQVRRA
                     ALVNLLGAVDEINVQGEIQQKLDVYANETLIDCFSLRDSIGIIASEENEHPITVGHTS
                     PNAQYAIIFDPLDGSSNIDVAVTIGTTFSIFRRPDDGSDGDPLQWVLQPGRKQIAAGY
                     VVYGSSTVLVYSIGNGVHGFTLDPSVGSYILTHENIRMPERGSYYSINEAYCGKFPEV
                     CNRFIEKLKSGALGRNYSLRFVGSMVADFHRTLLRGGIFLYPTTGSFPQGRLRLLYEA
                     NPIAFLAQQAGGLATDGKQNILDVEPIDLHQRTALMVGGKFEMTEFERCCSEI"
     gene            complement(5851..6501)
                     /gene="eda"
                     /locus_tag="VGB68_17410"
     CDS             complement(5851..6501)
                     /gene="eda"
                     /locus_tag="VGB68_17410"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEY0051075.1"
                     /translation="MNKSEVISQITGTGVIPVIRAASADEARRAIDAIKTGGIDIFEI
                     TMTVPGAVSLIEKLVASYGAEALIGAGTVLDAATARECIDAGAQFIISPALNLDTILF
                     CNQAETVVMPGALTPTEILVAWNAGADFVKVFPAGALGGASYLKSLKAPLPHIKLIPT
                     GGVSLQTAADFIRAGASAVGVGADLVDLEALRNNQDDLIAERAREYLKIVRTARQE"
     gene            complement(6508..>7346)
                     /locus_tag="VGB68_17415"
     CDS             complement(6508..>7346)
                     /locus_tag="VGB68_17415"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0051076.1"
                     /translation="NVGAIVAPLTVPLIAVNYGWQWAFIITGALGFIWLVFWLMIYRQ
                     PEEDPKLTPAEFAHIQSDLEEPVAKIPWRRLLPHRQTWAFAIGKFLTDPIWWVYLFWL
                     PKFLNTKFNLNILELGLPLIVIYVIADVGSIGGGWLSSYLIRRGWTVNAGRKTAMLIC
                     AIAVVPIVFASMTDNLWVAVCLIGLAAAAHQGWSANIFTTASDMFPRQAVGSVVGIGG
                     MAGSIGGMLIASTVGLILQYTNNSYFSVFIIAGSAYLIALLIIHLLAPNLEPAQIESS
                     AA"
BASE COUNT         1814 a         1912 c         2036 g         1584 t
ORIGIN      
        1 tattccgttg ttttcggcga agataattac gacgacatcg ccaaccagtt cgtctggatg
       61 gaaaagcacg cccgcgaccc gaaaaccgga ttgctttatc acggctggga cgaatcgcgc
      121 cggcaaaaat gggcgaacaa ggaaaccggc ttgagcccgc acgtctgggg acgcgcgatg
      181 ggctggtacg cgattgctct ggtcgatgtt ttggattatt tcccgaaaaa tcatccgcgc
      241 cgcggcgagc tcgtcgccat tttgcggcgc gaagccgaag cgattacgaa atatcaggac
      301 aaggacggct tgtggtggga cattatcgat ttggctggaa aagataaaaa ttatcacgag
      361 tcgtcagcgg cggcgatgtt cgtctacgcg ctggcgaaag gcgtccggca gggaaatctg
      421 cctgataaat atttaaatac ggcgcagaaa ggctgggcag gaatcaggaa agaatttatc
      481 agggaaaggg ctgacggaac gctcgactgg gaaggcacgg tttcggtttc cggcttgggc
      541 ggaaatcctt atcgcgacgg cagctacgat tattatatga gcgaaaagct gcgcacgaac
      601 gacgccaaag gcttgggtcc ggcggtaatg gcggcaatcg agatggaaaa tctcgaaaga
      661 ggcagaaccg gcgcgggcaa aaccgttctg ctcgacgatt atttcaaccg cgaagtccgc
      721 aaagacggtt ccgtttggca ttacaaatgg aacgagaaga atcatccggg tttttacgcc
      781 tggggcaaac aatttgaatc ttacggcgcg gcgctcgaca ctctttcgag cgcgccgacc
      841 gccgcaaatt tgaaaaacgc ggacgtttac atcatcgtcg accccgacac ggaaaaagaa
      901 accgaaaagc cgaatttcgt ttcgcgggcg gatgccggga cgattgccgg ctgggtgaag
      961 gcgggcggag tgctggtttt gatgggcaat gatttcggta attcggaatt tgataatttt
     1021 aacgttctgg cgaaacaatt cggcatcgaa tttaacaggg acagcaaata tcgagtgcag
     1081 aataacaatt ttgcggaagg caaaattacg gttgccggct cgaatccgat ttttaaaact
     1141 gcaaaaacgc tttatttgaa ggagctttcg accttaagcg tttcgacgcc agccggagcg
     1201 attctggaag cgaacggcga caaaattatg gcggcggcaa aatacggcaa gggaacggtt
     1261 ttcgccgtcg gcgacccgtg gctttacaac gagtatgtcg acggcagaaa gctgcccgcc
     1321 gaatacgaga atcttaaagc ggcgaacgat ttgagcgcgt ggctgctcgc ccgggcgcga
     1381 aagaaaaaat aacggcgcga aaaattatga taaaaaatat atttggaatt ttcgttttaa
     1441 cctgcgcttt gacgctcggc tcggctgcac agacgcgcga gcccgccgcg ccgattcgtc
     1501 cgtttgtcgt tttgaaaaag caatcgctcg gcgatttgca gaagaaattg agcgtcgata
     1561 acaaagtcga agatttaatc ggcggcgcgg gaatggagtt gcgcgtcgcc gtccagcacg
     1621 acaggacgcg cgaatccgcc gaggcggaaa gccacgacgc ttccgacgac gtttattacg
     1681 tactggaagg cgcggcgatg ctcacgctcg gcggaaagct ggaaaatccg cgcgaaacgt
     1741 cggcgggcga atggcgcagc gcgcgcattg tcggcggcga gcggtttgaa ataaaaaaag
     1801 gcgatttgat aatcgttccg cgcggcacgc cgcatcatcg cgtgaacgct aaaggaaagc
     1861 agttctcttt gattttaatc aaaatttttg ccgacccgct gcccgccgcc cgcgcgcgaa
     1921 actaaaatta aaaggcagat tgcgccgtcg tcgtaagtca gattaaagcc gtcgccgcct
     1981 catctaaaaa atttcgacaa aactttacat ctgcgctact ataaagctaa gttggagcga
     2041 gcgtgcaaaa tccttcggcg gcttcgacat aaagattaaa tgtcagactc gaaacacgac
     2101 atttttaaaa cgatagaaat cgaaaagggc atcaacacct cggaagaggt cgtttcgcag
     2161 ctgcgaaata tgattcacag cggcgcgctt tcggcgggcg accgcctgcc gcccgagcgc
     2221 gacctcgcca aactgctcgg cgtcagccgc ccgactttgc gcgccggaat tcgctcgctg
     2281 gcaagcgtcg gaatcctgca atcgcggcag ggcgcgggaa ctttcgtcgc ccaagccgaa
     2341 gaatcgccga cgctcgacag cagccctctg cgcctgatgg cgtccctgca cgggtttact
     2401 tcggacgaga tgttcgaggt gcgcctgtcg ctggaaatga gcatcgccgg attagccgcc
     2461 gagcgcgcta cagccgagca gatgacgcag atggcggaag aggtggcggg aatgtacgct
     2521 tcgctcgacg agccggagca atatctcgtc cacgatatgc gctttcatca aatcatcgcc
     2581 gccgcttcgc aaaatcggat tttgacggct ttgatgaata tggtggcgac gattttgttt
     2641 gaatatcgca gcaaaaccgt taaacgcgcg aaagacctca aggaatcagc cgaacaccac
     2701 cggaacattt accgcgcgat gcgcgaccgc aatccggaag ccgcgcgcaa ggcgatgcgc
     2761 gaccatttgg tcgaaacgca gaaagcacaa cgtctggaag tcgaaggaaa tttcccgggc
     2821 gagaacgggc acgggaaaaa atcctgaaaa tgcttgcgcc cgcagctttt caaatttttt
     2881 agaaaacaat ttttcgccgg gttttctttt atatcgcctg atataagagc cgggcttttt
     2941 tcgcgcaaat ttatgaaacg taaaattctt atcgttataa tcggactgct gtcgattttt
     3001 tcgcccgata cggcggcgca gccgacgaaa tcgaaagtct gggtcgccga caacggtgac
     3061 ggaacatata aaaatcctat cctgcacgcc gattattccg acccggacgc gattcgcgtc
     3121 ggcgaggatt tttatatgac ggcttcgagt tttaacgccg cgcccggttt gcccgttctg
     3181 cattcgaagg atttaatcaa ctggcgtttg ataaattacg tttttacgag acagccgcct
     3241 tttgaagttt tcgataaacc gcagcacggc ggcggcgttt gggcgccgtc gattcgctat
     3301 cacgacggcg aattttatat tttctatccc gacccggatt tcggtattta tatgacgaaa
     3361 acgaaaaatc cggcgggcga atggtcaaag ccgctgttga ttaaagaagc taagggctgg
     3421 attgatccgt gcccgctctg ggacgcggac ggcagcgcct atctggtcag cgcgtttgcc
     3481 ggaagccgcg ccgggataaa gaccgtttta atcgtcagcc gtatgagcgc ggacggaacg
     3541 cggctgctgg acgacggcgt gatggttttt gacgggcacg acgcgcaccc gaccgtcgaa
     3601 ggtccgaagt tttacaagcg caacggtttt tattatattt tcgctccggc gggcggcgtc
     3661 ccgaccggct ggcagacggt cttgcgttca aaaaatatct acggacctta tgacgaaaaa
     3721 atcgttctgg cgcaaggctc gacccagata aacggaccgc atcagggcgc ctgggtcgac
     3781 acgccgacgg gcgaagactg gtttctgcat tttcaagaca agggcgctta tggcagaatc
     3841 gtgcatctac agccgatgaa atgggtcgac gattttccgg tgatgggaat tgacaaagac
     3901 ggcgacggga cgggcgagcc ggttttgagt ttcaaaaaac cgaacgtcgg gaaaacattt
     3961 ccggtcgaaa cgccgccgga atccgatgaa tttaatgatg caaagctcgg cttgcaatgg
     4021 cagtggcagg cgaattttca gccgacgtgg gcgtttacct acccgccgaa aggctttttg
     4081 aaaatgttct cggtcgagct gccggaaaat tataaaaatc tgtgggacgt gccgaacctg
     4141 cttttgcaga aatttcccgc cgaagaattt accgtgacga cgaaagcgac tttgacgccg
     4201 cggtttgaag gcgaaaaatt cgcgctcgtc gtggcgggac tcgattattc atacatcggc
     4261 gtgacgaaca aaggcggcag gctttatgtc gcgcaggcga cggcgcgcga tgctgacaag
     4321 ggaaacgcgg aagcggaaaa cgtgccgttt ctgctgaacg aaaagacgtt ttacctgcgc
     4381 gtgaaagttt ccaaaggcgc gatgtgcagc ttcagcttca gcgccgacgg caaaaatttt
     4441 acgaatgccg gcgtgccgtt caaagcgcgc cagggaaaat ggatcggcgc gcgcgtcggt
     4501 ttgtttttta ccagaaaagg aaaatttaat gacgccggtt cagccgatgt cgattggttt
     4561 cggatagaat aattcaggat tcaagattca ggattcagga ttcaggattt aagattcaag
     4621 attcaagatt caagattagt cgtttgctaa cggcaaatcc tgaatcctga atcctgaatc
     4681 cacaatcata tgattaccac gcttcaacaa cacgtcgtgc aggaacaggc ggcaatgtcc
     4741 gattcgccgg acgagctgca atggatattt tccggtctgg cgctggcgac gaaaatgatt
     4801 caggcgcagg ttcggcgcgc ggcgctggtc aatcttttag gcgcggtcga cgaaatcaac
     4861 gtgcagggcg aaatccagca aaagctcgac gtttacgcca acgaaactct gatagattgt
     4921 ttcagcctgc gcgacagcat cggcattatc gcttcggaag aaaacgagca tccgataacc
     4981 gtcgggcaca cttcgccgaa cgcgcagtac gcgattatct tcgacccgct cgacggctct
     5041 tcgaacatcg atgtcgccgt caccatcggc acgacctttt cgatttttcg acgcccggac
     5101 gacggcagcg acggcgaccc tctgcaatgg gttttgcagc cgggcagaaa gcagattgcc
     5161 gccggttacg tcgtctacgg ttcttcgacc gttctggttt acagcatcgg caacggcgtt
     5221 cacggcttta cgctcgaccc ttcggtcggc tcgtatattc tgacgcacga aaatattcgg
     5281 atgcccgagc gcggcagtta ttactcgata aatgaagcgt attgcggcaa atttcccgag
     5341 gtctgcaatc gttttatcga aaaactgaaa agcggcgctc tgggcagaaa ttattctctg
     5401 cggtttgtcg gctcgatggt cgccgatttt caccgcacgc tgctgcgcgg cggcattttt
     5461 ctatacccga cgaccggcag tttcccgcaa ggcagactgc ggcttttata cgaggcgaat
     5521 ccgattgctt ttctggcgca gcaggcgggc ggattggcga ccgacggaaa acaaaatatt
     5581 ttagacgtcg agccgattga tttgcatcag cgaacggcgc tgatggtcgg cggcaaattc
     5641 gagatgacgg aattcgagcg ttgctgttcc gaaatataat cgaaacggca aaggcggaaa
     5701 ccggagcctt gacggagcgc attctttagc gattcgaggc gctgaattct gacgcgctcg
     5761 ttcacattcg catttccgcc tttttgcttt atttcattgt aatttgcttt agggctcgac
     5821 gcgattaggt tctgagcctg agcgtttcgc ttattcctga cgcgccgttc gaacgatttt
     5881 cagatactcg cgcgctcttt cggcgattaa atcgtcctga ttgtttcgca aagcttccaa
     5941 atcgaccaaa tccgcgccga caccgacggc gctcgctccg gctctgatga aatccgccgc
     6001 cgtttgcagg gaaactccgc ccgtcggaat taattttatg tgcggcagag gcgctttcaa
     6061 cgatttcaga tagctcgcgc cgccgagcgc tccggcgggg aaaactttga cgaaatctgc
     6121 gcccgcattc caggcgacaa ggatttccgt gggcgtcagc gcgccgggca tcacgaccgt
     6181 ctccgcctga ttgcaaaaca ggatcgtatc gagatttaac gccgggctga tgataaattg
     6241 cgctccggca tcgatgcatt cgcgcgccgt cgccgcgtcc aaaaccgttc ccgcgccgat
     6301 taaagcttcc gccccgtatg acgcgacgag cttttcgatt agcgaaaccg cgcccggaac
     6361 ggtcatcgtt atttcaaaaa tatcgatgcc gcctgtcttt atcgcgtcaa tcgctcgcct
     6421 cgcttcgtcc gccgacgcgg cgcgaatgac gggaatgacg cccgtcccgg taatttgact
     6481 gattacctcg gatttattca taaagcctta agccgccgaa ctttcaatct gcgccggttc
     6541 gagattcggc gccagcagat gaataatcaa cagcgcaatc aggtatgccg aacccgcgat
     6601 tataaaaacc gaaaaatacg agttgttggt gtattgcaga atcaagccga ccgtgctggc
     6661 gataagcatt ccgccgattg agccagccat tccgccgatg ccgacgaccg agccgaccgc
     6721 ctgccgcgga aacatatccg aagcggtcgt gaaaatattt gccgaccagc cctgatgagc
     6781 cgccgccgcc agcccgatta aacaaaccgc gacccagaga ttatccgtca tcgaagcgaa
     6841 aacaatcggc acgaccgcaa tcgcgcaaat cagcatcgcc gttttcctgc cggcgttgac
     6901 cgtccagccg cgcctgatta aatatgacga aagccagccg ccgccgattg agccgacgtc
     6961 ggcgataacg taaatgacga tgagcggcaa gccgagctcc agaatgttta aattgaattt
     7021 cgtgttcaga aacttcggca gccagaaaag ataaacccac caaatcgggt cggtcagaaa
     7081 ttttccgatg gcaaacgccc acgtctgccg atgcggaagc agacgccgcc agggaatttt
     7141 tgcgaccggc tcttccaaat ccgattgaat atgcgcaaat tcggcgggcg tcaatttcgg
     7201 gtcttcttcc ggttgtcgat aaatcatcag ccagaaaacg agccagataa aaccgagcgc
     7261 gcccgtaatg ataaacgccc attgccagcc gtaattaacg gcaatcagcg gcacggtcaa
     7321 aggcgcgaca atcgcgccga cgttcg
//
LOCUS       DASWNE010000888         2951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_207527, whole genome shotgun sequence.
ACCESSION   DASWNE010000888 DASWNE010000000
VERSION     DASWNE010000888.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207527"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VGB68_17420"
     CDS             <1..208
                     /locus_tag="VGB68_17420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051077.1"
                     /translation="IAEIIAQRSEQFETREFNRQLFSELCIQSAVTFEKYLRRRRNFT
                     IYCRISAVSTAGATCFSISIYLKL"
     gene            341..>2951
                     /locus_tag="VGB68_17425"
     CDS             341..>2951
                     /locus_tag="VGB68_17425"
                     /inference="COORDINATES: protein motif:HMM:NF024972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease family protein"
                     /protein_id="HEY0051078.1"
                     /translation="MQKKLSLYIVLILAINLLVAGTLAAQSAQNGVWQQISDSALQQR
                     TDVERQIIPRFYSTFQLDKAALRQILNRAPKEFSDPARGGNEVILTIPMPNGGFSRFR
                     IENSSIMEPELAAKYPEIQSYRGQGIDNPTETSRFDISPNGFRAMIYSNQGTILIEPY
                     AWGDTLNYISFFKSDVPRSSDPWICMTGEDIVMPQTEQISFVPAAPEVTSGATLRTYR
                     LAMAATGEYTSVFRQAGDTDAQAKARALAAINTSVNRINQVYERDVAIRMILVANNDL
                     IIYTDAASDPYTNVSDNATLSTNTTTLNEVIGTANYDIGHVFNTDGGGVASLNGPCGG
                     SKARGTTGLPNPIGDAFDIDYVAHEVGHQFGANHTFNSTIGGCASPNRSSSNAFEPGS
                     GITIMAYAGLCGSNNLGFNSIDTFHVRSIEAIVNYTTTLTGNTCAAQTPTGNTVPTVP
                     SINGSSFNIPKQTPFSLAATSTDANGDALTYDWQQYDAGGSTGGASTVPNSDADGIAR
                     PIFRPYLPTTNGTRTFPRLTHILNNANVPPNTTGGLMTGEILPAITRTMNFQVIVRDN
                     RSGGGGVNTAAVIVNVDGNSGPFAVTAPNTAVTWNGGSTQTVTWNAANTAAAPVNAAN
                     VKISLSTDGGQTFPTTLLAGTANDGTETITVPNITTTQARIKIEAVGNIFFDISGANF
                     TINQAAVVAKAPYDFDGDGKTDLAVFRPADSVWYLQRSQAGFTAAQFGVATDKTVAAD
                     FDGDNKTDIAVWRPANGTWYRLNSSNGTFVGAQFGANGDIPQPADFDGDGKDDLAVWR
                     PLTGEWFRINSGNGQFVGAQFGASGDRPVVGDYDGDAKADMTVFRPSNGTWYRINSSN
                     GAFSGVSFGLASDL"
BASE COUNT          716 a          819 c          774 g          642 t
ORIGIN      
        1 catcgcagaa attatagcgc aaagaagcga acagtttgaa acacgagaat ttaatagaca
       61 actttttagt gaattatgta ttcaaagcgc agtaactttt gaaaaatatt tgcgccgtcg
      121 ccgaaatttt acgatttatt gtcggatttc agcagtttct acagccggtg caacttgttt
      181 ttcgatttca atttatttaa agttataagc actcattcta caaccgattt atctaattgt
      241 tcctcgttgt agcttttcat aatcaataaa acaagcaggg tcggcaatca tttgattaat
      301 ctccaaattt tttgggatat attttaagga gtcacgataa atgcagaaaa agctttccct
      361 ttatattgtc ttgattttgg caataaattt actcgtcgcc ggcacgcttg cggcgcagag
      421 tgcgcagaac ggcgtctggc agcagattag tgattcggcg cttcagcaaa gaaccgacgt
      481 tgagcgacag attattccgc ggttctattc gacattccaa ttggataaag cggcgctgag
      541 acaaattctg aatcgcgcgc cgaaagaatt ttccgacccg gcgcgcggcg gcaatgaagt
      601 tattctgaca attccgatgc cgaacggcgg tttctcgcgt ttcagaatcg aaaactcttc
      661 gattatggaa ccggaactgg cggcaaaata tcccgaaatt cagagctatc gcgggcaggg
      721 aatcgacaac ccgaccgaaa ccagccgctt cgacatttcg cccaacggct tccgcgcgat
      781 gatttactcg aatcaaggca cgattctcat cgagccctac gcctggggcg atacgttaaa
      841 ctacatcagt ttcttcaaga gcgatgtgcc gcgctcaagc gacccgtgga tttgcatgac
      901 gggcgaagac atcgtcatgc cgcagaccga gcagattagt tttgtgcccg cagcgccgga
      961 agtgacgagc ggagccactc tcagaaccta tcgtctggcg atggcggcaa cgggcgaata
     1021 cacatcggtt ttccggcagg cgggcgacac cgacgcgcag gcgaaagcca gagcgctggc
     1081 ggcgattaac acatcggtca atcgcatcaa tcaagtgtat gaacgcgatg tcgcgatcag
     1141 aatgatttta gtcgccaaca acgatttgat catttacacg gacgcggcga gcgaccctta
     1201 caccaacgtc agcgacaacg caacattgtc aaccaatacg acgactttaa acgaagtcat
     1261 cggcacggct aattacgata tcggtcacgt atttaacacc gacggcggcg gcgtcgcttc
     1321 gcttaacggt ccgtgcggcg gcagcaaagc ccgcggcaca accggcttgc cgaatccgat
     1381 cggcgatgct ttcgatattg attatgtcgc gcacgaagtc ggacaccagt tcggcgcgaa
     1441 tcacactttc aacagcacga ttggcggctg tgccagcccg aaccgcagtt cctccaacgc
     1501 ttttgagccc ggcagcggca tcacgattat ggcttacgcc ggattgtgcg gctcgaataa
     1561 tctgggtttc aacagtatcg acactttcca cgtcagaagc atcgaagcga ttgtcaatta
     1621 caccacgact ctgacgggca acacctgtgc ggcgcagacg ccgaccggaa atacagtgcc
     1681 gacagttccc tcgattaacg gcagctcctt taatattccg aaacagacgc cgttttcttt
     1741 ggcggcgact tcgaccgacg cgaacggcga tgcgttgaca tatgactggc agcaatatga
     1801 cgccggcggt tcgaccggag gggcttcgac tgttcccaat tccgacgccg acggcatcgc
     1861 gcgcccgatt ttccgaccgt atttgccgac gacgaacgga acgcgcactt tcccgcgcct
     1921 gacgcatatt ttaaacaacg ccaacgttcc gccgaatacg acgggcggtt tgatgaccgg
     1981 cgaaattctt ccggcgataa cgcgcacgat gaattttcag gttatcgtgc gtgacaaccg
     2041 ctcgggcggc ggcggcgtca acacggcggc ggttatcgtc aacgtcgacg gcaacagcgg
     2101 accgtttgcc gtcaccgcgc cgaacacggc agtgacgtgg aacggcggct cgacgcaaac
     2161 cgtgacgtgg aatgcggcga atacggctgc cgctccggtc aacgcggcga acgtaaagat
     2221 ttcgctttcg accgacggcg gtcagacttt cccgacgacg cttctcgccg gcacggcaaa
     2281 tgacggaacg gaaaccatta ccgttccgaa catcacgacg acgcaggcgc gcatcaaaat
     2341 cgaagcggtc ggaaacatct ttttcgacat ctccggcgca aactttacga ttaatcaagc
     2401 cgctgttgtc gcaaaagctc cttatgactt tgacggcgac ggcaaaaccg atttagcggt
     2461 cttccgcccg gcggattcgg tctggtatct gcagcgctcg caagccggct ttaccgccgc
     2521 ccagttcggc gtcgccaccg ataaaacggt cgccgccgat ttcgacggcg acaataaaac
     2581 cgatattgcc gtttggagac ccgctaacgg aacctggtat cgtctgaatt catccaacgg
     2641 aacatttgtc ggagcgcagt tcggagcgaa cggcgacatt ccgcagcccg ccgactttga
     2701 cggcgacggc aaagacgatt tggcggtctg gcgtccttta accggcgaat ggttccgcat
     2761 caatagcggc aacggtcaat tcgtcggcgc gcagttcggc gcaagcggcg accgcccggt
     2821 cgtcggcgat tacgacggcg acgcgaaagc cgatatgacc gtctttcgcc cgtcaaacgg
     2881 aacctggtat cgcatcaaca gctcaaacgg tgcattctcc ggcgtttcct tcggattggc
     2941 gagtgatttg g
//
LOCUS       DASWNE010000889         1757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_340954, whole genome shotgun sequence.
ACCESSION   DASWNE010000889 DASWNE010000000
VERSION     DASWNE010000889.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340954"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VGB68_17430"
     CDS             <1..693
                     /locus_tag="VGB68_17430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY0051079.1"
                     /translation="YGYLAFNPIIDLVLEILLGFGMVIVILEKVLGDVRVSNEKLREA
                     HEKLEHLVQTDPLTTAFNRHAFYGFLKKRGDAEKTVSGCVGFFDIDNLKPINDRYGHA
                     AGDLAIRAVVRAIRGVMRAEDLIFRWGGDEFFVIMVSMNEEMARSRMDRLEAMLTDIM
                     LEGTNERLTVGVSYGFKDFAEISELEQAVKCADEEMYRRKQERKEYARQMQMNLVSTV
                     HESQPSTVSITN"
     gene            complement(784..1518)
                     /gene="bshB1"
                     /locus_tag="VGB68_17435"
     CDS             complement(784..1518)
                     /gene="bshB1"
                     /locus_tag="VGB68_17435"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HEY0051080.1"
                     /translation="MSEVDILAIFAHPDDMELAVGGTLLKMKSLGYKTGTLDVTRGEM
                     GTRGTPEGRAGEALEAARILKLDVRENLQLPDGHVWVTEETRTKLVRVLRRLKPKLIL
                     THQMDDPHPDHNHIAELVRESARLASMQKYDGDFGQQRIDVPTVAHNTFSRRITPTFI
                     VDISDFLAEKMESIRAHASQFHNPKSGDLETRLSAKGFLDELENRSRYYGSLIGVKAG
                     EPFYVREALNVEDPIALLTRPMNLYS"
BASE COUNT          413 a          446 c          472 g          426 t
ORIGIN      
        1 tacggttatc tggcgtttaa tccgattatc gacctcgtgc tggaaattct gctcggtttc
       61 ggaatggtca tcgttattct ggaaaaagtt ctcggcgatg ttcgcgtcag caacgaaaaa
      121 ctgcgcgagg cgcacgaaaa gctcgaacat ctggtgcaaa ccgacccgct gacgaccgct
      181 ttcaaccgcc acgcttttta cggctttctg aaaaaacgcg gcgatgccga aaaaacggtt
      241 tccggctgcg tcggattttt cgacatcgat aatctgaaac cgataaacga ccgctacggt
      301 cacgcggcgg gcgatttggc gattcgcgcc gtcgttcgcg cgattcgcgg cgtgatgcgg
      361 gcggaagatt tgatttttcg ctggggcggc gacgaatttt tcgtgattat ggtcagtatg
      421 aacgaggaaa tggcgcggtc gcggatggac cggctggaag cgatgctgac cgatattatg
      481 ctggaaggca cgaacgagcg tttaaccgtc ggcgtttcct acggctttaa ggatttcgcc
      541 gaaatatcgg agcttgagca agccgtcaaa tgcgccgacg aagaaatgta tcgccgcaaa
      601 caggagcgca aggaatacgc ccggcagatg cagatgaatc tggtctcgac ggttcacgaa
      661 tctcaaccga gcactgtttc aattacgaat taaaaattac gaattacgaa ttacgaattg
      721 acttgaattt actcgaacgg tcgagtttct tcaatcattc gtagttttta attcgtaatt
      781 gtttcacgaa tacagattca tcggacgcgt cagcagcgca atcgggtctt cgacgttcag
      841 agcctcgcgc acgtagaaag gctcgcccgc tttgacgccg atcaggctgc cgtaatatcg
      901 cgagcggttt tcgagctcgt ccagaaaacc ttttgccgag aggcgcgttt cgaggtcgcc
      961 ggatttcggg ttgtgaaact gcgatgcgtg agcgcgaatc gattccattt tttccgccag
     1021 aaaatcggaa atatcgacga taaacgtcgg tgtgatgcgg cgcgagaaag tattatgcgc
     1081 gacggtcgga acatcgatgc gctgttgccc gaaatcgccg tcgtatttct gcatcgaagc
     1141 cagccgcgcc gattcgcgca ccaattcggc gatgtgatta tggtccggat gcgggtcgtc
     1201 catctggtgc gtcaggatta atttcggctt cagacggcgc agaaccctga ccagtttcgt
     1261 gcgcgtctct tccgtcaccc agacgtgacc gtcgggcagt tgcagatttt cgcgcacgtc
     1321 aagttttaaa atccgagccg cttccagcgc ttctccggcg cgaccttcgg gcgttccgcg
     1381 cgttcccatc tcgccgcgcg tcacatccag cgtgccggtt ttgtagccca gagatttcat
     1441 tttcaaaagc gtgccgccga cggcgagctc catatcgtcg ggatgcgcga aaatcgccag
     1501 tatatcaact tcactcataa gataaattta ccacacggag gacacagaga aaattagacg
     1561 aagatgaacg ggattatgga gatgaacaca aaatcactaa acatcttttt caaaatcgct
     1621 gtcaatcgcc ttcgtcctcg tttaatttct ttttgatatt ataaagctat gaaattaatc
     1681 gttctcggtt cgggcacggc ggttccgcat ccgcggagaa gcagctcggg ttactggctg
     1741 gaaaccgacg cgggcac
//
LOCUS       DASWNE010000890         1625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_134142, whole genome shotgun sequence.
ACCESSION   DASWNE010000890 DASWNE010000000
VERSION     DASWNE010000890.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134142"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VGB68_17440"
     CDS             complement(<1..216)
                     /locus_tag="VGB68_17440"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HEY0051081.1"
                     /translation="MATSGGKIDNYTKFWDFYVLEHSNPLTRWFHFTGTTLGLILLVW
                     FVQLGAWYYLPLCLVTGYAFAWFSHFFI"
     gene            complement(222..545)
                     /locus_tag="VGB68_17445"
     CDS             complement(222..545)
                     /locus_tag="VGB68_17445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051082.1"
                     /translation="MKIATFLITALVNAAVSFVMFFMLLLGLNGFSGDQAEPGLILYI
                     VWSFLSAIITGVSGIWLANYLATKKSMNKIAATMVSSLIFIAVGVVSVIVSFFAAVIL
                     TSAMR"
     gene            complement(655..1182)
                     /locus_tag="VGB68_17450"
     CDS             complement(655..1182)
                     /locus_tag="VGB68_17450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051083.1"
                     /translation="MPLNVIILFALISVFGAACANNFARKPEVEQSLYSVSGESCAIS
                     EDDIFTLPDRKVFGECEIRKNPERFNGKLLRIKARYNFMMHGALLESSAECGLSRSID
                     EAISIGFRSEKDYEIIRNLQALPVDIIAVGEFSASAPSRESDSIRDRTPYRFTGFCLE
                     EAKAADKKNNGSEPR"
     gene            complement(1232..>1625)
                     /locus_tag="VGB68_17455"
     CDS             complement(1232..>1625)
                     /locus_tag="VGB68_17455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0051084.1"
                     /translation="RQIGGDAHGFRFIQLPFNLAMPEALLLPNQQFGGQALSTVEAAR
                     NLDVSVACSASILQGKLARNLPANVRETLGNLESDALASLQFVRSTPFVTTALIGMSR
                     EKHVEENMRLARVEPVAAETYQQLFEQG"
BASE COUNT          426 a          449 c          365 g          385 t
ORIGIN      
        1 gataaaaaaa tgcgaaaacc aggcgaaagc gtagcctgtc acgaggcaaa gcggcagata
       61 ataccacgcg ccgagctgca cgaaccagac cagcagaatc aacccgagcg tcgtccccgt
      121 aaaatgaaac cagcgcgtca aaggattgct gtgttccagc acgtaaaaat cccagaattt
      181 cgtgtaatta tcaatttttc cgccgcttgt cgccataaat ttcacctcat tgccgatgtc
      241 aaaatcacag ccgcgaaaaa gctgacaatc accgagacga cgccgaccgc gataaaaatc
      301 aaactggaaa ccatcgtcgc cgcgatttta ttcatcgact ttttcgtcgc cagataattt
      361 gccagccaga ttcctgaaac gcccgtgatt atagccgaaa gaaagctcca gacgatatac
      421 aaaatcaaac ccggctcggc ttggtcgccg ctaaaaccgt tcagcccgag cagcagcata
      481 aagaacatca caaaactgac ggcggcgttt accagcgcgg tgattaaaaa tgtcgctatt
      541 ttcatgttta aaatttacga aaacgattgc cgctaagttt ccgtcaaaaa cgatttgatt
      601 tcaaccgccg tcccattttc ccaaaaatct tttttccggc ttcgaccggc gcgtttatcg
      661 cggctcgctt ccgttatttt ttttatccgc ggctttcgct tcttcgagac agaatcccgt
      721 aaaacgatag ggcgttctat cgcgaatcga atcgctttcg cgtgacggcg cggacgcgga
      781 aaattctccg acggcgatta tatcgacggg aagcgcctgt agatttcgga tgatttcata
      841 atctttttcc gaacggaaac cgatgcttat cgcttcgtca atcgagcgtg aaagcccgca
      901 ttctgcgctg ctctcgagta aagcgccgtg catcataaaa ttgtatctcg ctttgattct
      961 gagcagcttt ccgttaaatc gctcaggatt ttttctgatt tcgcactcgc cgaaaacttt
     1021 cctgtccggc aaagtaaaaa tatcgtcttc ggaaatcgcg cagctttctc cgcttaccga
     1081 gtaaagcgat tgttcgacct ccggtttccg ggcaaaatta ttcgcgcaag ccgcgccgaa
     1141 aaccgaaatc agcgcgaaca gaataattac atttaaaggc ataaacatcc tcataatttt
     1201 cagacgccgc ccgtttctga aatttttcga atcagccctg ctcgaaaagc tgttgataag
     1261 tttccgccgc aaccggctcg acgcgcgcca gacgcatatt ttcttcgacg tgtttttccc
     1321 tgctcatccc gattagcgcc gtcgtgacaa aaggcgtcga gcggacgaat tgcaggctgg
     1381 cgagcgcgtc cgattcgaga tttcccagcg tttcgcgaac gttcgcgggc agattgcgcg
     1441 ccagttttcc ctgcaaaatc gaagcgctgc acgcgaccga gacgtctaaa ttccgcgccg
     1501 cttcgacggt cgataaagcc tgcccgccaa actgctggtt aggcaaaagc agagcttccg
     1561 gcatcgccag attaaacggc agctgaataa atcgaaaacc gtgagcgtcg ccgccgattt
     1621 gccgc
//
LOCUS       DASWNE010000891         4410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_381179, whole genome shotgun sequence.
ACCESSION   DASWNE010000891 DASWNE010000000
VERSION     DASWNE010000891.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381179"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..498)
                     /locus_tag="VGB68_17460"
     CDS             complement(97..498)
                     /locus_tag="VGB68_17460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051085.1"
                     /translation="MNTKLLMISSALLMAVGGVLLQFFPHEILNRLGASSAGIAPLFA
                     QITGALYLGFAMTNWMAKTVLIGGIYARPLAVGNFAHFLIGALALLKYAFTVPDSRLV
                     WVLTVVYSLLAILFALVFFTHPLKTNNASQN"
     gene            complement(677..3322)
                     /gene="cphA"
                     /locus_tag="VGB68_17465"
     CDS             complement(677..3322)
                     /gene="cphA"
                     /locus_tag="VGB68_17465"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: protein motif:HMM:NF010623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEY0051086.1"
                     /translation="MKIELIKSFSGANIYSHQPVLMIELDLEELKGKKSREISGFNEQ
                     LLELLPNLYEHYCDSGKAGGFVERLRKGTHFNHVIEHAAVELLALAGIDLRHEKICNG
                     DEKDDSRAVIETTTVETTRYSMTAAAELVEALVSEKTFFIEEKIAEAKDIAAETELGP
                     SAAAIVEAAEKRGIPWRRENTSSLIQLGYGKNLRQVQAAITDVTGVIGADLAKDKDLT
                     KRRLSDFSIPVPDGEIVRTEQEAARALELIGAPVVVKPLDGRQGKGVSLNLKTPAEVI
                     EAFHIAREYSRKVLVEELFEGKNYRVLVVDGKMVAASERVPCGVIGDGEHTVGQLIDI
                     ENQNPLRGEGHEKPLTKIKLDPIILSALQKEGWKMEDVPPAGERVSLCAGMNLSTGGT
                     ARDVTDEVHRSVKTLCERAARVIGLDICGVDLVLEDISAPMPKEKGGVIEINAAPGLR
                     MHTFPSEGKPRDVGGAIIEMLYPNGKQARIPIISVTGTNGKTTVTRMISHILNDGSLT
                     IGTTTSTGIYLNGELIVEGDTTGPISARTILGDKAVDVAVLETARGGIVRRGLAYDWS
                     DISVLTNITEDHIGQDGIESIEDLISIKSLIAERVRPGGTLILNADDETSRRVLEREN
                     LKRIDRQIVYFSLDENNARVREHAGRGGTAYVAKNNFIVEITGAESRRLVDSREIPVT
                     LNGTADFQTANAMSAIAACRAYGMSPEQIVSRLKTFRTDSDNPGRNNLYKVGKGYVLV
                     DYGHNPDGFAAICRMVSRWEGKTITGIINIAGDRDDRIVREAAKIAAGCFRRVIATED
                     IDRRGRAPGEIPRLMCETIRQENPDCHCDIMPDEAEAVSKAIREIAENEVIVIFYDRL
                     TTVLEILAQNRAVPVSSFEETASAQ"
     gene            3978..4259
                     /locus_tag="VGB68_17470"
     CDS             3978..4259
                     /locus_tag="VGB68_17470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051087.1"
                     /translation="MKKFITLSLLLGSMVFGVSSANAETNSAASLTANAVEPQIRIQI
                     GRNNRRVRRTVTTTRTIRVGRQRFRETIRTTYLPNGRTRTQIISRVRVR"
BASE COUNT          980 a         1204 c         1107 g         1119 t
ORIGIN      
        1 ggttagaagc ttctgccgtt atctttgccg cttcggagcc ggtttcggct ttgcctgcgc
       61 cgaagattgt ttcaaattaa atttaccgcc agtcggctaa ttttgagagg cgttgtttgt
      121 cttgagcgga tgagtaaaaa agacgagcgc aaataaaatc gccagcagcg aataaactac
      181 cgtcagaacc caaacgagcc gcgaatccgg aaccgtaaag gcgtatttga gcaaagccag
      241 agcgccgatg aggaaatgcg cgaaattgcc gacggcgagc gggcgcgcgt agattccgcc
      301 tatcaggacg gttttcgcca tccagttcgt catcgcaaag cccaaatata aagcgccggt
      361 aatctgtgcg aacaacggcg cgatgccggc agaagaagct ccgaggcgat tcagaatttc
      421 gtgcgggaaa aattgaagca aaacgccgcc gaccgccatc agcagggcgc tggaaatcat
      481 caataatttt gtattcatcc gaaagatgta cgccgccgcc cgcaaaaagt ttcagcccga
      541 ccaagagaag accgtgcttt gtacgggatt attttaaagg ctcgaagcaa tccgagaccg
      601 gctcgaaacc ggctctcaaa attgaacggc gacccgaagc ttcaggtcaa gatattttta
      661 aattcgcgcg actcgcctat tgagcgctcg ccgtttcctc gaatgacgag acgggcacgg
      721 ctcggttttg cgcgagaatt tccaaaactg tcgtcagccg gtcgtaaaaa ataacgatta
      781 cctcattttc cgcgatttcc cgaattgctt tggaaaccgc ttcggcttcg tcaggcataa
      841 tgtcgcaatg acagtccgga ttttcctgcc tgattgtttc gcacatcagg cgcggaatct
      901 cgccgggcgc gcgcccgcgc cggtctatat cttcggtggc gatcacgcgc cggaaacagc
      961 cggctgctat ctttgcggct tccctgacaa tacggtcgtc gcggtcgccg gcgatattga
     1021 ttattccggt tatcgttttg ccttcccagc gcgacaccat ccggcaaatc gccgcgaagc
     1081 cgtccggatt gtgaccgtaa tcgaccagca cataaccttt accgactttg tacagattgt
     1141 ttctgcccgg attgtcgctg tcggtgcgaa aggttttcag gcggctgacg atttgctcgg
     1201 gggacattcc gtaggcgcgg caggcggcaa tcgccgacat cgcgttcgcc gtctgaaaat
     1261 cggctgtccc gttcagagtg accggaattt ctctggaatc gacgagccgg cggctttccg
     1321 cgcccgttat ttcgacgatg aaattatttt tggcgacgta agccgtgccg cctcgcccgg
     1381 cgtgttcgcg cacgcgcgcg ttattttcgt cgagtgaaaa ataaacgatt tgccggtcga
     1441 ttcgctttag gttttcgcgt tccagaacgc gcctcgaagt ttcgtcgtcg gcgtttaaaa
     1501 tcaaagttcc gcccggacgg acgcgctcgg cgattaacga tttgatagag attaaatctt
     1561 cgatcgattc gatgccgtcc tgcccgatgt ggtcttcggt gatgttcgtc aaaacggaaa
     1621 tgtccgacca gtcgtaggcg agcccgcgcc ggacgattcc gccgcgcgcc gtttccagca
     1681 cggcaacgtc gaccgcctta tcgccgagaa tcgtccgcgc cgaaatcggt ccggtcgtgt
     1741 cgccttcgac aatcagctcg ccgttcaaat aaattccggt gctggtcgtc gttccgattg
     1801 tcaggctccc gtcgttgaga atgtgcgaaa tcatccgggt gacggtcgtt tttccgtttg
     1861 ttccggtgac ggaaataatc gggatgcgcg cctgctttcc gttcgggtaa agcatctcga
     1921 taatcgcgcc gccgacgtcg cgcggctttc cctcgctcgg aaaagtgtgc attcttaagc
     1981 ccggcgcggc gttgatttct atgacgccgc ctttttcttt cggcatcggc gcggaaatgt
     2041 cttccagcac gaggtcgacg ccgcagatgt ccagaccgat aacgcgcgcc gctctttcgc
     2101 acaatgtttt aacgctccgg tgaacctcgt ccgtcacgtc gcgcgccgtg ccgccggtcg
     2161 aaagattcat tccggcgcac aggctgacgc gctcgccggc gggcggcacg tcttccattt
     2221 tccagccttc tttctgcaaa gcggaaagaa taatcgggtc gagttttatt ttcgtcagag
     2281 gtttttcgtg cccttcgccg cgcagaggat tttgattttc aatatcaatc aactgcccga
     2341 ccgtgtgctc gccgtcgccg attacgccgc acgggacgcg ctcgctcgcc gcgaccattt
     2401 ttccgtcgac aactaaaacg cggtaatttt tcccctcaaa caattcttcg accagaactt
     2461 ttcgagaata ttcgcgggcg atgtgaaaag cctcgataac ttccgcgggc gtcttcagat
     2521 taagcgagac gccttttccc tgccgaccgt cgagcggctt gacgacgacc ggcgcgccga
     2581 ttaattcgag cgcgcgggcg gcttcctgtt cggtgcggac gatttcgccg tccggaacgg
     2641 gaatcgaaaa gtcgctcaaa cgccgtttgg tcaaatcctt atctttggct aaatccgcgc
     2701 cgatgacgcc ggttacgtca gttattgccg cctgcacctg acgcagattt ttcccgtaac
     2761 caagctgaat caggctggat gtattctcgc gccgccacgg aattccgcgt ttttcggcgg
     2821 cttcgacaat cgccgccgcg ctcggaccga gttccgtttc cgccgcgatg tctttggctt
     2881 ccgcgatttt ttcttcgata aaaaacgttt tttcgctgac cagggcttcg accagctcgg
     2941 cggcggcggt catcgaatag cgcgtcgttt cgacggtcgt ggtttcaatc acggctctgg
     3001 agtcgtcctt ttcgtcgccg ttgcagattt tttcgtgacg caggtcgatg cccgccagcg
     3061 ccagcaattc gacggcggcg tgctcgatga cgtgattaaa atgcgttcct ttgcgcagcc
     3121 gctcgacaaa accgcccgct ttgccggaat cgcaataatg ctcgtacaaa ttcggcagca
     3181 gctcgagcag ttgttcatta aagccggaaa tctcgcggct cttttttcct ttcagctctt
     3241 ccaggtcgag ctctatcatc agcaccggct ggtgactgta aatgtttgcg ccggaaaaag
     3301 attttatcaa ttcgattttc ataaccttgg ctttcccaat ctacttttta aatctccaac
     3361 tccttaatta tgcagtttga ttaaaagaat actcgtgcct cgacaaacat aaaaatattg
     3421 cccgctccga cgaaacggca attactgaaa gagacggcgg acgcctctat tttaatttcc
     3481 gccggacaaa acgaattaac gtttcaatga aagaataaaa agtgtccggc aaataaaagt
     3541 gacgcgaata gaattaataa aattccgctg taaattatca ccctttcatt tccgccgaga
     3601 gttctaaatt gcataagttt gtttccgcaa cccgcatttt atcagtctta gagcagatag
     3661 aatacgacgg caaattattg ttgtctgttc tgtataaaac atttctaatc ctcgccgacc
     3721 gcgcccggga ttcttcattt cgggattcgc ttttgagctt ttgtcgttca aaagctttgc
     3781 gtccggtcag gcgtcgccat tcgttaatag aatttttgtc tcaaaacgga taaatttatg
     3841 ccttttagtg ccggtcgcta aacgcccggc gacaagtatt cttttatatt cctatattta
     3901 acgctctggc acagctattg cttaatagag gtcaggtcac agacctgaat ttcaaatttt
     3961 aaaaggggta gaaaacaatg aaaaaattca taacgctttc attattactt ggttcgatgg
     4021 ttttcggagt ttcgtccgcc aacgcggaaa caaatagtgc cgcctcgctt acggcaaacg
     4081 cggtcgagcc gcagattcgc attcaaatcg ggcgcaacaa ccgtcgcgtg cgccgcacgg
     4141 tgacgaccac gcgcaccatt cgcgtcggca ggcagagatt tcgcgagact attcgcacca
     4201 cttatcttcc gaacggcaga acgcgcacgc agatcatcag ccgcgtcaga gttcgctaat
     4261 ccttcgggaa aacattacag ctaaatttaa aagccggtca gagcatttcg atttgaccgg
     4321 cttttcctat ttgtaaaata atgtcgccgg ttccggttaa tccgagcctt tatttctcta
     4381 tggtttgatg ctttgcacaa aaataaagga
//
LOCUS       DASWNE010000892         5577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_665942, whole genome shotgun sequence.
ACCESSION   DASWNE010000892 DASWNE010000000
VERSION     DASWNE010000892.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5577
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665942"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1336
                     /locus_tag="VGB68_17475"
     CDS             <1..1336
                     /locus_tag="VGB68_17475"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY0051088.1"
                     /translation="KGNYYISEPQKKIRYIFLSTSKIGEKTSIPEADLKAEYDKLPED
                     KRNSGVQAQQIVLRIAKPEFDAQVLEKANQIVERARSAEGGKISEQAFGELAQGQSED
                     PNTARAGGKISRPVTQNPNNPTDPLQRLFTLQEGEITEPIKFGEKYYILRRGALVPKS
                     FEDAKKELEVSLRNRRAYTIAAELAQKVTDDLKQTKNVEATAQKFAAEANMSVKDMVR
                     ETGFIKPGDEVENVGISPQFEEGIKTLENANDVGEKIPIKDGFGIPLLVEKKEPRDET
                     FEEAKNKVAEALKLEKARAQVEQIARDIAAGATGVEALNAAAQSKGLKAQESKSFILG
                     SPLGQGPSAATSEELENAIYALKAGEASKTPVKVGDNWYVVGVAKREEASTEEFAKQR
                     DQLLQSSLQQKRGQVFSDYLEDIRRRMEASQQIKIYKEALNKVDGMDADADA"
     gene            complement(1460..2164)
                     /locus_tag="VGB68_17480"
     CDS             complement(1460..2164)
                     /locus_tag="VGB68_17480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0051089.1"
                     /translation="MLTLENVSVNYGAIEALTDISLHVKQGEVVTLIGANGAGKTTTL
                     RTITGLLEPKSGRVVYEGKEINGMATHRLVPMGMVMSPEGRGVFANLSVRENLEMGAY
                     IRNDKAGIAKDMERGFTMFPRLKEREQQKAGTLSGGEQQMLAMARALMSRPRLLLLDE
                     PSLGLAPLVVHTIFQAIEEIRSEGTTILLVEQNANAALRHSDRAYVLETGRIIMEGDS
                     KTLANDPRVKEAYLGE"
     gene            complement(2208..2564)
                     /locus_tag="VGB68_17485"
     CDS             complement(2208..2564)
                     /locus_tag="VGB68_17485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEY0051090.1"
                     /translation="MLAKSFEDVEIWKKAHAWVLKIYRLSENFPKHELFGLISQLRRA
                     AISVPANFAEGFKKLGKADKLRFYNIAQGSLEECRYYLILARDLNYGEVSELQKDSEV
                     ISKMPDSYMKMIAKDK"
     gene            complement(2593..3363)
                     /locus_tag="VGB68_17490"
     CDS             complement(2593..3363)
                     /locus_tag="VGB68_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0051091.1"
                     /translation="MENGNHILQTDKATIRFGGLIAVNELTMQVQKGEIYGLIGPNGA
                     GKTTIFNLLTGVYEPTSGDIHIVGKRINGLRPYQISRLGVSRTFQNIRLFPSMSVLEN
                     VVTACHRHANQNMIDAVFRLPRYHRDEREHRKFALELLEIFDLAKYRNEGGTSLPYGN
                     QRRLEIVRALATRPQLLLLDEPAAGMNPSEQEDLTRLIKEIRDRFGLTIVLVEHNMRV
                     VMGVCDRIQVVDYGKSIALGLPQEIKNDPKVIEAYLGD"
     gene            complement(3420..4544)
                     /locus_tag="VGB68_17495"
     CDS             complement(3420..4544)
                     /locus_tag="VGB68_17495"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0051092.1"
                     /translation="MSSWLKSLIVGIILVVVLYVLNAMMSSSGFFGMGVDDYQARLLV
                     FICINIILATSLNLINGFTGQFSIGHAGFMAVGAYASAYFTVNYGKALEGSFGFMGET
                     GAASVVLLIAVFIGAVVAAIMGLIVGIPSLRLKGDYLAIVTLGFAEIIRIVILNIDAV
                     GGATGYAVPGYASFLWIGLFTIITLVVIHNIVKSDSGRALISIREDELAAEAMGVNVT
                     RYKVTSFVVGSAFAGIAGVLFAHYNKFLHTNDFQFIRSFEIIIMIVIGGMGSMTGAIL
                     GAIIVTLLPELLRQLPAMQVGATTFQFADLRLVIFALILILTMILRPQGILGTREFGL
                     NWLKRPRKTDEGNAAVGGNAGVAEAQQEEAKRLQSEEDKK"
     gene            complement(4627..>5577)
                     /locus_tag="VGB68_17500"
     CDS             complement(4627..>5577)
                     /locus_tag="VGB68_17500"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY0051093.1"
                     /translation="SPLSALSTFYFMDTFVQQLINGLTIGSIYALIALGYTMVYGILR
                     LINFAHGDIYMVGAYAGFILATALGFAASPSVFGFIVTLLGAMAIAAAIGMAIERFAY
                     RPVRKYSKMTTLITAIGMSLLIEYLFIFFFSPTPRSFPQLINNDNYTLFGNAKISTTQ
                     ILIFVVSVLLMIALQYIIFYTKIGKAMRAVSFNLNSAKLMGINTDFVIAFTFALGSAL
                     AAAGGVLTAQYNPKIDPLMGILYGLKAFVAAVLGGIGNIPGAVLGGLVIGAAETLVVG
                     YGGYIGVPSTYRDAVAFAILILVLLIRPAGLLGSNVQEKV"
BASE COUNT         1484 a         1494 c         1408 g         1191 t
ORIGIN      
        1 caaaggcaat tactatattt ccgagccgca gaaaaagatt cgctacattt tcctgagcac
       61 ttcgaaaatc ggcgaaaaga cttcgattcc cgaagccgat ttgaaagccg agtacgacaa
      121 gctgccggaa gacaaacgaa actccggcgt gcaggcgcag cagattgttt tgcgcatcgc
      181 caagccggaa tttgacgcgc aggtcttgga aaaagccaat caaatcgtcg aacgcgcccg
      241 cagcgccgag ggcggaaaaa tttccgagca ggctttcggc gaattggcgc agggtcaatc
      301 agaagacccg aacacggcgc gcgcgggcgg aaaaatttcg cgtcccgtga cccaaaaccc
      361 gaataatccg accgacccgc tgcagcggct ttttacgctg caggaaggcg aaatcaccga
      421 accgattaaa ttcggcgaaa aatattacat cctgcggcgc ggcgcgcttg ttccgaaatc
      481 gtttgaagac gcgaaaaagg aactcgaagt cagcctgcgc aatcgccgcg cttacacgat
      541 tgccgccgaa ctggcgcaaa aggtcaccga tgatttgaag caaaccaaaa acgtcgaagc
      601 gacggcgcag aaatttgccg ccgaagcgaa tatgagcgtc aaggatatgg tgcgcgagac
      661 gggctttatc aaaccgggcg acgaagtgga aaacgtcggc atctcgccgc agttcgaaga
      721 aggcatcaag actctggaaa acgccaacga cgtcggcgaa aaaatcccga ttaaagacgg
      781 tttcggcatt ccgctgctgg tcgagaaaaa agagccgcgc gacgagactt tcgaagaagc
      841 gaaaaacaaa gtcgccgaag ccttaaagct ggaaaaagcg cgcgcgcagg tcgagcaaat
      901 cgccagagac atcgcggcag gcgcaaccgg cgtcgaagcg ctcaacgccg ccgcccaatc
      961 gaaaggcttg aaggcgcagg aatccaagag ctttattctc ggctcgccgc tcggacaagg
     1021 tccgtcagcc gccaccagcg aagaactgga aaacgcgatt tacgcgctta aagcgggcga
     1081 agcttcaaaa acgccggtta aggtcggcga taactggtat gtcgtcggcg tagcgaaacg
     1141 cgaagaagcc agcacggaag aattcgccaa acagcgcgac cagcttttgc agtcgtcgct
     1201 ccagcaaaaa cgcgggcagg tcttttcgga ttatctggaa gacatccgcc gccggatgga
     1261 agccagccag caaatcaaaa tttacaagga agcgctcaat aaagtcgacg gtatggacgc
     1321 cgacgcggac gcttaagatt taaggcaaag gtaaaaaggc ggaagaaatt tcttctgcct
     1381 ttgcatattc taaaacgaaa aagacgcgga gagtttttaa gctttccgca tctttttcgt
     1441 tttcaatggc gtaaaaaatt tattcgccca ggtaagcttc cttgacgcgc gggtcgttgg
     1501 cgagcgtttt cgaatcgcct tccatgatta ttctgccggt ttccagaaca tacgcgcggt
     1561 cggagtgacg cagcgcggcg ttggcgttct gctcgaccag cagaatcgtc gtgccttccg
     1621 agcgaatttc ttcaatcgcc tgaaaaatcg tgtgaacgac cagcggcgca agcccgagcg
     1681 acggctcgtc gagcagcaaa agacgcgggc gcgacatcaa tgcgcgcgcc atcgccagca
     1741 tctgctgctc gccgccggaa agagttccgg ctttctgctg ctcgcgttcc ttcaaacgcg
     1801 ggaacatcgt aaaaccgcgc tccatatcct tggcgattcc ggctttgtca ttgcggatat
     1861 acgcgcccat ttccagattt tcgcggacgc tcagattggc gaaaacgccg cgcccttccg
     1921 gcgacatcac cattcccatc ggcacgagcc ggtgcgtcgc catcccgttg atttccttgc
     1981 cttcatagac gacgcgaccg cttttcggtt cgagcaatcc ggtaatcgtg cgcagcgtcg
     2041 tcgttttgcc cgcgccgttt gcgccgatta aagtgacgac ttcgccttgt ttgacgtgca
     2101 gtgaaatgtc ggttaaggct tcgattgcgc cgtaattgac tgatacgttt tctaaagtca
     2161 acataatttt taagaagccg gaagatagaa gtcagaagcc tgtagaatta tttgtctttt
     2221 gcaatcattt tcatatagct gtcaggcatc ttgcttatta cttccgaatc tttctgtaat
     2281 tcactaactt ctccgtagtt taaatctctg gctaaaatca aataataacg acattcttcc
     2341 agtgagcctt gagcgatatt ataaaaacgt agtttgtccg ctttgcctaa ttttttaaag
     2401 ccttccgcga aatttgccgg aactgaaatt gcggctcgac gtaattgtga aatcagaccg
     2461 aaaagttcat gttttggaaa attctcggaa agcctgtaaa ttttcaaaac ccaggcgtga
     2521 gcctttttcc aaatttctac atcctcaaaa ctcttcgcaa gcattttcta ctgtcttccg
     2581 acttccgact tcctaatctc caagatacgc ctcgataact ttcgggtcgt ttttaatttc
     2641 ctgcggcagt ccgagcgcga tcgatttgcc ataatcgacg acctgaatgc ggtcgcagac
     2701 gcccatcacg acgcgcatat tgtgttcgac cagcacaatc gtcagaccga agcggtcgcg
     2761 gatttcctta atcaaacgcg tcaaatcttc ctgctcggac ggattcattc ccgccgccgg
     2821 ttcgtcaagc agaagaagct gcgggcgcgt cgccagcgcg cggacgattt ccagtcgccg
     2881 ctggtttccg tatggcaggc tggttccgcc ctcgttcctg tattttgcca aatcgaaaat
     2941 ttccagcagc tcgagcgcaa atttgcgatg ctcgcgctcg tccctgtgat agcgcggcag
     3001 gcgaaaaacc gcgtcaatca tattttgatt ggcgtggcgg tgacaagccg tgacgacgtt
     3061 ttccagaacg ctcatcgagg gaaacaggcg aatattttga aacgtgcgcg agacgccgag
     3121 ccgcgagatt tgatacggtc gcaagccgtt gatgcgtttt ccgacgatgt gaatatctcc
     3181 ggatgtcggc tcgtaaacgc ccgtcaaaag attaaaaatc gtcgttttgc ctgcgccgtt
     3241 cggaccgata agaccgtaaa tctcgccctt ttgaacctgc atcgtcagct cgttgacggc
     3301 aatcagaccg ccgaaacgaa tcgtcgcttt atcagtttgc agaatgtgat ttccgttttc
     3361 cattggtaaa tttttataag tgaaaagtcg tcagcgaaaa gctttttact tttcactttt
     3421 tactttttat cttcctcgct ttgcaaacgt ttggcttctt cctgctgggc ttcggcgacg
     3481 cccgcatttc cgccgaccgc cgcattgcct tcatccgttt tgcgcgggcg cttgagccag
     3541 ttcagaccga attctctggt tccgagaatt ccctgcgggc gcagaatcat cgtcaaaatc
     3601 aaaatcaaag cgaaaatcac gaggcgcaaa tcggcgaatt gaaacgtcgt cgcgccgacc
     3661 tgcatcgccg gcagctggcg caaaagctcg ggcagcagcg tcacgataat cgcgcctaaa
     3721 atcgcgcccg tcatcgagcc cattccgccg atgacaatca taataataat ctcgaacgaa
     3781 cggataaact gaaaatcgtt ggtgtgcaga aatttattat aatgcgcgaa aagaacgccc
     3841 gcgatgcccg cgaaagccga gccgacgaca aaggaggtga ctttatagcg cgtcacgttg
     3901 acgcccatcg cttcagccgc cagttcgtcc tcgcgaatgg agataagcgc gcgccccgag
     3961 tcggacttga cgatattgtg aatcacgacg agcgtaatta tcgtaaaaag tccaatccag
     4021 agaaaactgg cgtagcccgg aacggcatag ccggtcgcgc cgccgaccgc gtcgatattt
     4081 aaaatgacga tgcggataat ctcggcaaat ccgagcgtca cgattgccag ataatcgccc
     4141 ttcaggcgca aactgggaat gccgacaatc aagcccataa tcgccgcgac gacggcgccg
     4201 ataaaaaccg cgattagaag cacgacgctc gcagcgcccg tctcgcccat aaatccgaac
     4261 gagccttcga gcgctttgcc gtaattgacc gtaaaataag ccgacgcata agcgccgacc
     4321 gccataaatc cggcgtgccc gatggaaaac tgcccggtaa agccgttaat cagatttagc
     4381 gatgtcgcca ggattatgtt gatgcagata aaaaccagca ggcgcgcttg ataatcgtca
     4441 acgcccatac cgaaaaatcc gctgctgctc atcatcgcgt tcaaaacata gagaacgacg
     4501 acgaggatga ttccaactat taaactcttc aaccaggaag acatatattt tagtggatag
     4561 tggatagttg atagttgata atgaaagata aaaactctca gttatcagtt atcagttatc
     4621 aactaattaa actttttcct gcacattcga gccgagcaaa cccgccggac gaatcaatag
     4681 aaccagaatc aaaatggcaa atgcgacggc gtcgcgataa gtcgacggaa cgccgatgta
     4741 gccgccgtag ccgaccacca gagtttccgc cgcgccgatg accagcccgc ccagaaccgc
     4801 gcccggaata ttgccgatgc cgcccagaac cgccgcgaca aatgccttta agccgtaaag
     4861 aatgcccatc agcgggtcga ttttcggatt atactgcgcc gtcaaaacgc cgcccgccgc
     4921 cgccagcgcc gaccccagag caaacgtaaa ggcaatcacg aaatccgtgt tgatgcccat
     4981 caattttgcc gagttcaggt taaacgagac agcccgcatc gccttgccga ttttcgtgta
     5041 aaaaatgatg tattgcagag caatcatcag caaaaccgaa acgacgaaaa ttaaaatctg
     5101 cgtcgtggaa atcttcgcgt tgccgaaaag cgtgtagtta tcgttgttaa tcaactgcgg
     5161 aaacgagcgc ggcgtcggcg aaaaaaagaa gatgaaaaga tactcgatta aaagcgacat
     5221 tccgattgcc gtaatcagcg tcgtcatttt tgaatacttt ctaacggggc gataagcgaa
     5281 gcgctcgatt gccatcccga tggcggcggc aatcgccatc gcgccgagca gcgtgacgat
     5341 aaagccgaaa accgacgggc tggcggcaaa gccgagcgcc gtggcgagaa taaagcccgc
     5401 gtatgcgccg accatatata tgtcgccgtg cgcaaagtta atcaggcgca gaatgccgta
     5461 aaccatcgtg tagccgagcg caatgagcgc gtaaatcgag ccgatggtca agccgttgat
     5521 aagctgctga acaaaagtgt ccataaaata gaaagtagag agagcggaga gcggaga
//
LOCUS       DASWNE010000893         4809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_29749, whole genome shotgun sequence.
ACCESSION   DASWNE010000893 DASWNE010000000
VERSION     DASWNE010000893.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29749"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VGB68_17505"
     CDS             <1..371
                     /locus_tag="VGB68_17505"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEY0051094.1"
                     /translation="EVAAEKRVRTLVINWWSFAAEETKAVFGEDGGHAGWNETAYIQA
                     IDPTLVKKERYKDDLATAYPAAGTWQAVPFPSSIGLYQKGQGYPKFDQQKADEYFRKV
                     NDKVANLINEIIRKWDMAGL"
     gene            complement(523..1395)
                     /locus_tag="VGB68_17510"
     CDS             complement(523..1395)
                     /locus_tag="VGB68_17510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051095.1"
                     /translation="MSLITCPDCGNACSLEAVACPQCGRPLAPPIVEPIPQREVIIQE
                     PRVEKSFPPWAIALIAILGAGLLFALFMMMRGNSDEDNRNLSVNVSQRESANLRTPRV
                     ETGSSATDVTVPRTVPPSQSSEVTVPPSQSTSINTPPTDTRTATADNVPVGKSSLSVE
                     AKIRGTTGREQAVQKETFYLLKKDLRSILEEADIEDEEGQGVVNAFGMSIVYPGRYPE
                     TRRKALAAISKYTVAKTLTDAQGKAKFPDVKPDSYYLFGITKTPEGFSVWDQPITLSE
                     GQMPNLILQPSGGR"
     gene            1562..2167
                     /locus_tag="VGB68_17515"
     CDS             1562..2167
                     /locus_tag="VGB68_17515"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY0051096.1"
                     /translation="MAVTVQTQEVRESILDATDRILSSRGYKKMTIDDLAQEVGIGKG
                     SVYLHFTSKEEIALSHIDRIVERLKEKLHRIAAENDAPENRFKKMLICRVLHRFDSVQ
                     HYTVSINDLLAAIRPRLLERRQKYFAEEAQIFAGIVVEGQLNGVFQDGDALEIAETLM
                     TATNSLLPVSLSTEELGERDEIEGKITRLADLLLSGVLKRA"
     gene            2332..3435
                     /locus_tag="VGB68_17520"
     CDS             2332..3435
                     /locus_tag="VGB68_17520"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY0051097.1"
                     /translation="MKNSIIALAVMVLFVLDIYGQQNANQPPDLPIDAATKAQVVNDL
                     TRELNESYVFAETAKKMETDVRQRLQNKEYDKITSSREFADKLTSDLQAVSRDKHLRV
                     RFSYEKIPARQDRREPSKEELENQQRYVRQINFGFEKIERLQGNIGYVDLRGFSDAEA
                     GVETVAAAMNFLTNTDALIFDLRQNGGGSPEMVALISSYLFGEKPVHLNSLYWRKGDK
                     TEEFWTKAAVAGKRYGETKDVYVLTSNRTFSAGEEFTYNLKNLKRATIIGETTGGGAH
                     PGGMFRINDHFGAFVPSGRAISPVTKTNWEGTGVEPDVKVSKEIALKTAYAMALNKVA
                     DKTTDEQRKRALKELADKAQKEIDEMKKVAKTN"
     gene            3721..4188
                     /locus_tag="VGB68_17525"
     CDS             3721..4188
                     /locus_tag="VGB68_17525"
                     /inference="COORDINATES: protein motif:HMM:NF019896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HEY0051098.1"
                     /translation="MVNLNMSLSENFKLLEFVTSVTASNHGIDNSPNNTEIEHLRILC
                     RKILQPARRAAGPLKISSGFRSEQLNRLVGGAPDSDHRRGFAADVVPLSGLTRELAVW
                     VARNCREFDQIILEFGTDSRPEWIHLSVAPKLRRQILRATKKDGQTIYRAITL"
     gene            complement(4203..4661)
                     /locus_tag="VGB68_17530"
     CDS             complement(4203..4661)
                     /locus_tag="VGB68_17530"
                     /inference="COORDINATES: protein motif:HMM:NF042704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-phosphate processing receiver
                     domain-containing protein"
                     /protein_id="HEY0051099.1"
                     /translation="MSILRSLLMKLGLVNLKADRSPITVFLLDDDRRRHRWFSKRFDG
                     DELDIAETVEEAKELLAENSYDAIFLDHDLLPHHYESNDHDDFATTGYAIAEWLHENK
                     DLQRAATIIVHTRNADAAIRMVQKLRDSGRNCEYCAFPMLDLKIRNYWKR"
BASE COUNT         1299 a         1192 c         1223 g         1095 t
ORIGIN      
        1 cagaagtagc ggctgagaaa cgggtgcgaa cgctggtcat aaattggtgg tcgttcgctg
       61 ccgaagaaac caaagcggtt ttcggcgaag acggcgggca cgcgggctgg aacgaaacgg
      121 cgtatattca ggcgattgac ccgacgctgg tgaaaaagga aagatacaag gacgatttgg
      181 cgaccgcgta tccggcggcg ggaacgtggc aagccgtgcc gtttccctct tcaatcgggc
      241 tttatcaaaa aggtcagggc tacccgaaat tcgaccagca aaaagccgac gaatatttcc
      301 gcaaagtcaa cgacaaggtg gcgaacctga taaacgaaat cattcgcaaa tgggatatgg
      361 cgggactttg aaaggcgaaa ggctgaaggc gaaaggcgaa attaagaaaa gttttaattt
      421 ttgaattttg aattattcat ttagttgcaa atgcaagtag ctgcaaattc aaaattcaaa
      481 aattaaaatt aaaattcaaa atctttcgcc tttcgccttt aactatcgcc cgccgctcgg
      541 ctgtagaatc aaattcggca tttgtccttc gctcagggta atcggctggt cccagacgga
      601 aaagccttcg ggcgttttcg tgatgccgaa aagataataa ctgtccggtt tcacgtcggg
      661 aaatttggct ttgccctgcg cgtcggtcaa agttttggcg acggtgtatt tgctgattgc
      721 cgccagcgcc ttgcgtctgg tctccgggta tctgccggga taaacgattg acataccgaa
      781 agcattgact actccctgac cttcctcgtc ttcaatatcg gcttcttcca gaatactccg
      841 caaatctttt ttcaaaagat aaaaagtttc tttctggacg gcttgttccc tgccggtcgt
      901 tccgcgaatt ttcgcctcga cgctcaaaga gcttttgccg accggaacat tgtccgccgt
      961 cgccgtgcgc gtatcggtcg gcggcgtatt gatgctcgtg ctctgcgagg gcggaacggt
     1021 cacttccgag ctttgcgaag gcggaacggt tcgcggaacg gtgacgtccg tcgcgctcga
     1081 accggtttcg acgcgcggcg tccgcaaatt tgccgattct ctttgagaga cattgacgct
     1141 gagattgcga ttgtcctcgt cgctattgcc gcgcatcatc ataaacaacg cgaaaagcaa
     1201 gcccgcgcct aaaatcgcaa tcagagcaat cgcccacggc ggaaagcttt tttccacccg
     1261 cggctcctga atgataactt cgcgctgcgg aatcggctcg actatcggcg gcgccagcgg
     1321 acgaccgcac tgcgggcaag cgaccgcttc cagcgaacag gcattgccgc agtcgggaca
     1381 agttattaaa gacatttgta atttcctccc tttactcgtt cgttgtcgaa acaacgttta
     1441 tctgatttaa gaataataca tttcgccgta aaaatgttta taaatctttt ttgaaactaa
     1501 ttcgaccgat ttggcgttta tcttcttgac taaaattcag tttcagtcaa acatagtaaa
     1561 tatggctgtt acagttcaaa cacaggaagt acgcgagtcg attctggatg cgaccgaccg
     1621 cattctttcc agtcgcggct acaagaaaat gacgattgac gatttggcgc aggaagtcgg
     1681 catcggaaaa ggcagcgtct atctgcattt tacgagcaag gaagaaatcg ccctgtcgca
     1741 catcgaccgc atcgtcgaaa ggttaaagga aaaactgcac cggattgccg ccgaaaacga
     1801 tgcgcccgaa aatcgtttta agaaaatgct aatctgccgc gttctgcacc gcttcgacag
     1861 cgtccagcat tacacggtca gcatcaacga tttgctggcg gcgattcgcc cgcgtctgct
     1921 ggagcggcga cagaaatatt ttgccgaaga agcgcagatt ttcgccggaa tcgtcgtcga
     1981 aggacagctc aacggagttt ttcaggacgg cgacgcgctg gaaatcgccg aaactctaat
     2041 gacggcgacc aattcgcttc tgccggtcag tctcagcacc gaagaactcg gcgagcgaga
     2101 tgaaatcgaa gggaaaatca cgcggcttgc cgatttgctt ctcagcggcg ttttaaaacg
     2161 cgcgtaaaaa atttatgccc agtcaagcca taaatttttg tttttaatcg tctgcctcgg
     2221 atgaggcggg caaatgagcg ggacgctttc tcaaggcgtt caaaacacga gccggaaatt
     2281 tcaaagttca caacgtttta tataaacgtt tcatataaag gagttataaa catgaaaaat
     2341 tcaatcatcg cactagccgt aatggtttta ttcgtgctgg acatttacgg tcagcaaaac
     2401 gccaatcagc cgccggattt accgattgac gcggcaacca aagcacaagt cgtcaacgac
     2461 ctgaccagag agctcaacga atcctacgtt ttcgccgaaa ccgcgaaaaa gatggagacg
     2521 gacgtcaggc aaagactgca aaacaaggaa tacgataaaa tcacgagctc cagagagttt
     2581 gccgataaat tgacctccga tttgcaagcc gtgagccgtg acaaacacct gcgcgttcgt
     2641 ttttcttacg aaaaaattcc cgctcgccag gacagacgcg agccttcaaa agaagaattg
     2701 gaaaatcaac agcgctacgt tcgccaaatc aatttcggtt ttgaaaaaat cgagcgcctg
     2761 cagggcaaca ttggatacgt ggatttgcgc ggcttttccg acgcggaagc gggcgtcgaa
     2821 accgtcgcag cggcgatgaa tttcctgacc aacacggacg ctctgatttt cgatttgcgg
     2881 cagaacggcg gcggcagccc ggaaatggtc gcgctgatca gctcgtatct tttcggcgaa
     2941 aaacccgtcc acctgaacag cctctactgg cgcaaaggcg ataaaacgga agaattctgg
     3001 acgaaagccg ccgtcgccgg caaacgctac ggcgaaacga aagacgttta cgttctgacg
     3061 agcaaccgca ctttcagcgc cggcgaagaa ttcacctaca acctgaaaaa tctgaaacgc
     3121 gcgaccatta tcggcgagac gaccggcggc ggcgcgcatc cgggcggaat gtttcgcatc
     3181 aacgaccatt tcggcgcatt cgtcccgtca ggacgcgcca tcagccccgt tacgaaaacg
     3241 aattgggaag gcacaggcgt cgagccggac gtaaaggttt cgaaagaaat cgcgctcaag
     3301 acggcttacg cgatggcgct gaataaagtc gcggacaaaa caaccgacga acagcgaaaa
     3361 agagccttga aagaattagc cgacaaggcg cagaaggaaa tcgacgagat gaaaaaagtc
     3421 gctaaaacca attagacggg ctgaaagctg aaacgcaaaa aggcaacccg cgcgggttgc
     3481 ctttttgcat ttctccgggc ttttttattg acactaaatt ttcgtctgtg ttatcgtgct
     3541 atccaatcta cgaccgggac gctaatttca ggctgaagct tgtgatttcc ggctgtcaat
     3601 aaaccggcga aacctgctgt tttttgcagt cgaataaaaa aattcttcaa aacgttaaat
     3661 cattcgaagg gctttgttcg atttctttca ccgaaaataa aaggagataa aataaaatat
     3721 atggttaatc tgaatatgtc tctgtcggaa aacttcaagc tgctggagtt tgtgacatcc
     3781 gttacggctt cgaatcacgg tattgataat agtccgaaca atacggaaat cgaacatctc
     3841 cgcatattat gccgaaaaat tctgcagccc gcccgccgtg ccgccggacc tttaaaaatc
     3901 agttcgggat ttcgctccga acagttaaac cggctggtcg gcggcgcgcc cgacagcgac
     3961 caccggcgcg gctttgcggc ggacgtcgtg ccgctgagcg ggctgaccag agaattagcc
     4021 gtctgggtag ccagaaattg ccgggagttt gaccagataa ttctggaatt cggcacggac
     4081 agccgccccg aatggattca tctgagcgtc gcgccaaagc tgcggcgaca gattttaaga
     4141 gcgaccaaaa aagacggtca aacaatctac cgggcgataa cgttgtagag cttgtttgaa
     4201 aattatcgtt tccaatagtt tctaatcttc aaatccagca tcggaaacgc gcagtattcg
     4261 cagtttctgc cggaatcgcg cagcttctgc accatccgaa tcgcggcgtc ggcgttgcgc
     4321 gtgtggacga taatcgtcgc cgcgcgctgc aaatccttgt tttcgtgcag ccattcggca
     4381 atcgcgtagc cggtcgtggc gaaatcgtcg tggtcgttcg attcgtaatg atgcggcaaa
     4441 aggtcgtggt cgagaaaaat cgcgtcgtaa ctgttttccg ccaaaagctc tttcgcttcc
     4501 tcgaccgttt cggcgatgtc gagctcgtcg ccgtcgaatc ttttggaaaa ccatcggtga
     4561 cggcggcggt cgtcgtcgag cagaaaaacc gttatcgggc tgcggtcggc tttcagattg
     4621 accaagccga gtttcatcaa caaactgcgt aagatgctca taattcaaca actaaacgaa
     4681 agctttaata taacacacga cggcgaccgt gaaaatttgg cagggagtga ctaaatgagc
     4741 gtagttaaga taaactttgt tgatgcagtc ctgtccaaat tgtgaaaact gtgagaaaca
     4801 aataaaaag
//
LOCUS       DASWNE010000894         3149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_666060, whole genome shotgun sequence.
ACCESSION   DASWNE010000894 DASWNE010000000
VERSION     DASWNE010000894.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666060"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1535
                     /gene="uvrA"
                     /locus_tag="VGB68_17535"
     CDS             <1..1535
                     /gene="uvrA"
                     /locus_tag="VGB68_17535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY0051100.1"
                     /translation="DYTNLTIEESIERIGEISLSQREEKIAGLVLKEIKDRLGFLQAV
                     GLGYLTLERPSATLSGGEGQRIRLATQIGSQLRGVLYVLDEPSIGLHPRDNQKLLETL
                     HELRDLGNTVLVVEHDEETIEKADYVVDLGPGAGTHGGEVVAEGTPADIEKNADSITG
                     QYLSKKLAIEIPELRRLPNVNGITVKGARAHNLKNIDVRFPLGIFTVVTGVSGSGKST
                     LVEDILYPALARHVYGSLVEPLEHDEIEGLELVDKVIEIDQTPIGRTPRSNPATYTGL
                     FTPIRELYAMLPESRERGYKAGRFSFNVKGGRCEACDGDGMKRIEMNFLPDVYVLCDV
                     CRGARYNRETLAVKYKGLSIADLLDTTIEDALPILENIPQIKQKLQTLLDVGLGYIKI
                     GQSSTTLSGGEAQRIKLAKELSKRATGKTIYILDEPTTGLHFADVHKLLDVLQRLVDT
                     GNTVIVIEHNLDVIKSADYVIDLGPEGGEGGGKLVATGTPEDVVAVRKSYTGKALISV
                     LN"
     gene            1694..2992
                     /gene="murA"
                     /locus_tag="VGB68_17540"
     CDS             1694..2992
                     /gene="murA"
                     /locus_tag="VGB68_17540"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY0051101.1"
                     /translation="MDKFLIRGGKPLRGKIAISGAKNSALPCLAAALLSAETVTLHNV
                     PYVKDLITQRRLLEDLGATVLTPELRTHKITARNIEVFEAPYHLVKTMRASVLALGPL
                     LARFGQAKVSLPGGCAIGTRPIDLHLKAFEQLGAIVSLEAGDVVARAPSGRLIGADVS
                     FEKVTVTGTENVMMAAVLARGKTTIHNAAREPEIEDLAELLNKMGARVNGAGTSTIEI
                     EGVEALNGAEHTIIPDRIETGTFIVAAAITGGELEITNCEPEHLRAAIGKMREAGAEI
                     EELNPSTLLVRCGGKGLAAKEVKTEPHPNFPTDMQAQYMSLMTQAEGVSVIQETIFEN
                     RFMHASELVRMGAKIEISGNTAKVHGKTALSGAPVIASDLRASASLVLAGLAARGETL
                     IDRVYHIDRGYENIVKKLGAVGANIERVTDNLTMTATEEI"
BASE COUNT          791 a          798 c          876 g          684 t
ORIGIN      
        1 ccgattatac aaacctgacg attgaagaat cgatcgagcg aatcggagaa atttcgctca
       61 gccagcgaga ggaaaaaatc gccggtctgg tgttaaagga aatcaaagac cggctcggat
      121 ttctgcaggc ggtcgggctc ggttatctga ctctggaaag accgtcggcg actttgagcg
      181 gcggcgaagg gcagcgcatt cggctggcga cgcaaatcgg ctcgcaattg cgcggcgttc
      241 tttatgtttt ggacgagcct tccatcgggc tgcacccgcg cgacaatcaa aaactgctgg
      301 aaactctcca cgaattaaga gatttgggca acacggttct ggttgtcgag cacgacgagg
      361 aaacgattga aaaagccgat tatgtcgtcg atttaggacc gggcgcggga acgcacggcg
      421 gagaagtcgt cgccgaagga acgcccgccg acatcgaaaa aaacgcggat tcgataaccg
      481 ggcaatatct ctccaaaaag ctcgccatcg aaattcccga attgcggcgc ttgccgaacg
      541 tcaacggcat caccgtcaaa ggcgcgcggg cgcacaatct gaaaaacatc gacgtgcgct
      601 ttccgctcgg cattttcacg gtcgtgacgg gcgtttccgg ctcgggtaaa tcgacgctcg
      661 tcgaagacat tctttacccg gcgctggcgc gtcacgtcta cggctcgctg gtcgagccgc
      721 tcgaacacga cgagatcgag ggcttggaat tagtcgataa agtcatagag attgaccaga
      781 cgccgatcgg cagaacgccg cgctcgaatc cggcgaccta caccggactt ttcacgccga
      841 tccgcgagct ttacgcgatg ctgccggaat ccagagaacg cggctacaaa gccggacgtt
      901 tttcgtttaa cgtcaaaggc ggacgctgcg aagcctgcga cggcgacgga atgaaacgca
      961 tcgagatgaa ttttttgccc gacgtttacg tgctctgcga cgtttgccgc ggcgcgcgct
     1021 acaaccgcga aactttagcc gtcaaataca aaggtctttc gattgccgat ttgctcgata
     1081 cgacaatcga agacgctctg ccgattctgg aaaacattcc gcagattaaa caaaaactgc
     1141 aaaccctgct cgacgtcggg ctcggctaca tcaaaatcgg tcagtcttcg acgaccttga
     1201 gcggcggcga agcccagcgc atcaagctgg cgaaagaatt atcgaaacgc gccaccggaa
     1261 aaacgattta cattctcgac gaaccgacga ccggcttgca tttcgccgac gtccacaaat
     1321 tattggacgt tttgcaaaga ttggtcgata cgggcaacac ggtcatcgtc atcgaacaca
     1381 atctggacgt gataaaatca gcggattacg tcattgattt aggacccgaa ggcggcgaag
     1441 gcggcggaaa gctggtggcg accggcacgc ccgaagacgt ggtcgccgtg cgaaaatcct
     1501 ataccggaaa agctttgata tcggttttga attgattttg aaatccagaa aaaacgagtt
     1561 tgtatgaagt ctttaattag ctcctgcaaa ctcgtttttt atttgcctca aaaaatagtt
     1621 attaattttc tttcatatat tgctcgtgat aagatttgtt tttaactttc ataagtgata
     1681 gttgtcgtaa acgatggata aatttttaat tcgcggcgga aagccgcttc gtggaaaaat
     1741 tgcgattagc ggcgcgaaaa attcggcttt gccttgtctg gcagcggctc ttttatcggc
     1801 tgaaaccgtt actttgcata atgttccgta tgtcaaggat ttgattacgc agcgtcgtct
     1861 gctggaagat ttgggcgcga cggttttgac gcccgagctg cgcacgcaca aaattacggc
     1921 gcgaaacatc gaggttttcg aagcgccgta tcacctggtc aagacgatgc gggcgagcgt
     1981 tctcgccctc ggtccgctgc tggcgcgttt cgggcaggcg aaagtcagct tgccgggcgg
     2041 ctgcgccatc gggacgcgac cgattgattt gcatctgaaa gccttcgagc aattgggcgc
     2101 gattgtttcg ctcgaagcgg gcgatgtcgt tgcccgagcg ccgagcggaa gattaatcgg
     2161 cgcggatgta tcgtttgaaa aagtgacggt cacgggaacg gaaaacgtga tgatggcggc
     2221 ggttctggcg cgcggtaaaa cgacgattca caacgcggcg cgcgagccgg aaatcgaaga
     2281 tttagccgag cttttaaata aaatgggcgc gcgcgtcaac ggcgcgggaa cttccacaat
     2341 cgagattgaa ggcgtcgaag ccttaaacgg cgcggagcac acaatcattc ccgaccgcat
     2401 cgaaacggga acttttattg tcgccgccgc gattaccggc ggcgagcttg aaatcacgaa
     2461 ttgcgagccg gaacacctgc gcgccgccat cggaaaaatg cgcgaagccg gcgcggaaat
     2521 cgaagagctg aatccgagca cgctgctcgt cagatgcggc gggaaaggtt tagccgccaa
     2581 agaggtgaag accgagccgc acccgaattt tccgaccgat atgcaggcgc agtatatgtc
     2641 tctgatgacg caagccgaag gcgtttcggt gatacaggag acgatttttg aaaatcgttt
     2701 tatgcacgct tcggagctgg tacggatggg cgcgaaaatc gaaatttccg gcaacacggc
     2761 aaaagttcac ggcaaaaccg cgctgtccgg cgcgcccgtt atcgcttcgg atttgcgcgc
     2821 ctcggcgtcc ctggttctgg cggggctggc ggcacgcggc gaaactttga ttgaccgcgt
     2881 ttatcatata gaccgcggtt atgaaaatat cgtcaaaaaa ctcggcgcgg tcggcgcgaa
     2941 tatcgagcgc gtaacggata atttgacgat gaccgcaaca gaagaaatct aaagagttgt
     3001 aaatttctaa agaaaaagcg aaggaatcaa cgcattgatt ccttcgcttt tcaaatttaa
     3061 tgattgagct ttttttctcg cctttaccaa ccccaagttc ccggagcgcc tttgaaaggt
     3121 ccgacaatat cttttgttat ccaaccgcc
//
LOCUS       DASWNE010000895         8328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_277669, whole genome shotgun sequence.
ACCESSION   DASWNE010000895 DASWNE010000000
VERSION     DASWNE010000895.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8328
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277669"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1843)
                     /locus_tag="VGB68_17545"
     CDS             complement(<1..1843)
                     /locus_tag="VGB68_17545"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0051102.1"
                     /translation="MIEVKEITNKHTNKSKSEEILSKPAVIDAKASLSFTEEMKGYIT
                     EGETDYDAGYRTGESAETFIKFRLTISIDDVNRFITDAAHEAAAAGVVSCESLWGDSE
                     VEKGIFNLFVDTENPSVKHMLYRLYFRDAEGPLTLSGHKLIRDDPGFDVWEDTTTLFT
                     KIFRGHVSAEEEARAEVYAAGIMHIYMLDFLKQLSTFRVDAPRLSDRAQALSAFGKFF
                     VGTLWDIYISRVPQPSENGNGDKPGSAAQGGKDAVGNGRAVPLFTLEGVKDAEVSTHY
                     FSTEDKLGLSMLRFNRAACDDVVLIIHGLTTSSDMFIMPEHYNLVSYLLDNGFTDVWC
                     LDYRMSNRLPYNLAPHRFNMDDIALFDYPAALAKIREQIGNRRLHVICHCLGAVSFTM
                     SLFGKAVDNITSVIANSAALTPRVPGWSKIKLNVAPFLVEFIVGFPYINTRWSEDPGI
                     TRGKVFNKVVSLFHRECDVPACHMLSMMWGTGWPALYSHENLHDVTHRRGGDLYGGTS
                     MHYYRHVRRMYKAGRAVKYAPKEQKYNRLPDDYLQHARDIETPVLFMTGGNNRVFTDS
                     NIHCYNELEQLTPGRHQLHVFPNYGHQDVFMGKNCHLDIFPRMLQFLN"
     gene            complement(1836..3530)
                     /locus_tag="VGB68_17550"
     CDS             complement(1836..3530)
                     /locus_tag="VGB68_17550"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY0051103.1"
                     /translation="MDFEVVIVGSGFGGSVMAYRLAKAGYKVCVLERGKSYPPGSFPR
                     SPLAMQNNFWDPSKGLQGLYNVWSFRGSEALVASGLGGGSLIYANVLLRKDTAWFTEA
                     DLIGRDELDFHYDNVEAMLKPQKYPMDFSPYNKTPKTLALKFAAEELMKQNPQNIEWM
                     LPNLAVMFAADQNQKPALAEPLLPDSHNIHKLQRYTCRLCGECDIGCNYGSKNTLDHT
                     YLSEAVRLGAEIRTRCEVRSFRPGDEAGYVIDYVVHEPENEGREFDTAKLPSKTITSD
                     KLVLAAGTLGTTYLLLKNRKAFNGRLSKTLGTRYCGNGDLLTFVMKSKITADGKKIPR
                     VINANYGPVITSAIRGADITEGGSGPGFYIQDAGFPEFLNWVIQSVDLGNSLKRYARF
                     LSRYICGVLGLNTDSDLSAEIAEVFTASKYSYASLPLLGMGLDSPDGNMKLDAKEKYL
                     DVDWNKRGSNPLFKQLTKTSRKIADILQAKFQENPLMYLSRTITVHPLGGCPMGINPA
                     MGVVNSFGEVFNCPGLYIADGSVMPGPVGANPSLTIAALADRFAAELIRKESKTDD"
     gene            complement(3975..5723)
                     /locus_tag="VGB68_17555"
     CDS             complement(3975..5723)
                     /locus_tag="VGB68_17555"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY0051104.1"
                     /translation="MKSSNFYYYSTLFAYCFGYYCAAGLAINIAYHRCLSHRSLRLWK
                     PLERVLVTLGLPAGTPVQWVGNHRFHHRHTDVESDPHSPVTDGFWHSHVGWYIGTKNP
                     LACFLYSIAGPLRTIYDGWNRPRTNQEHNRLAADVAGDGYYRFVSRPAFFALACWLHA
                     LVFYGISYFVWGRVGFILLWITTVIIYNLGDAIDSVAHLYGKRPFQANHFARNNHLLG
                     ILTLGEGWHANHHVFPSSARHGLLANQFDAMWKVVCLFEFIGAASEIKLPTAEQIQSK
                     LLSPPQERKKDNLMLPEKYSKQFDINKFDVADPDPWMALYVDRSIQIDDEAKAALLIS
                     MRSKSRFLFPLIKPFLWLSLHAITLFRIIIPNLFTSSKLLHRMIYIGLKYFVSPEANF
                     IILRHFHIGSEILEFIAENVPDVEIKLNPLRPEKLEDVLDEIFLKHDLNLFNFIINLN
                     IELRELDRQIETPEKLNFNSITDGQFPMERFPKRWTNFLDVQSAIEIYTPIYQLFLSG
                     ASFWRASNSLQLDETVGLYVSRLLGNNQYLGLLNNKHPLVPLPTSGAGYRLLLHGLGS
                     EMLHAILREHKRKQNK"
     gene            complement(5736..7658)
                     /locus_tag="VGB68_17560"
     CDS             complement(5736..7658)
                     /locus_tag="VGB68_17560"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEY0051105.1"
                     /translation="MLPKNSVYITSFGKFLPGEPVANNEIEDYLGLIGGRQSRLKSRI
                     LRQNGIRTRHYAIDAGGKVGFWNYELAANAVIDSLKQSEIDAKDVQFLAASTSQSDLL
                     APGFASLVHGASRLPPCEIASFQSICAGSIMALQAAFTGILSGVRENSVVCGSEFTSR
                     FFRPGLFEETASFQKNGKLPFDAEFLRWMLSDGAGAAVLENRPNRKGVSLKIEWIDLI
                     SYGSSFDVCMYSGANKQADGTLEKSWYDYPNFAAAARDGALLLKQDVKILENVLKLGV
                     KRYFELIEQGRINSKDIDWFVCHYSSHVFRSKIIELLEKGNGMISEERWFTNLYTKGN
                     TGAASIFIMLEELFGSGKLQPGQKILCMVPESGRFVVSFMMLTVVETAAERQNEILGE
                     ADVIDAAAAEQHSISPFVDKNKHYAEKAELQSTLWRKLTDVWLDFESRLNQIPIIERL
                     NRGKLRIEDYRNLLLNLRQQVVEGGRWISRAASNITAEAFPLRSLFIGHAKEEHRDFQ
                     MLERDFVSVGGSLEEIQKAEKNIGSEALSAWMFHCSSRENPFELLGAMFIIEGLGQKM
                     ANKWGNRIREQLNLQAAQVSFLLYHGANDENHLEKLEQALASGILNAELVERIVKTAK
                     VTARLYALQLEEIGNF"
BASE COUNT         2182 a         2045 c         1910 g         2191 t
ORIGIN      
        1 catttaggaa ttgaagcatt ctcgggaaaa tatcgagatg acagttttta cccataaata
       61 cgtcctgatg cccgtaattc gggaaaacgt gcaactgatg cctgccgggc gttaactgtt
      121 cgagctcatt gtaacaatga atgttcgagt cggtgaatac gcgattattt cctccggtca
      181 taaacaaaac aggggtttca atatctctcg catgttgcag gtaatcgtcg ggcaggcgat
      241 tatatttctg ctccttcgga gcgtatttaa ccgcccgccc cgccttatac atgcgccgca
      301 cgtgtctgta ataatgcatg ctcgtcccgc cgtataaatc gccgccccgg cgatgagtta
      361 catcgtgtaa attttcgtga ctgtataaag caggccatcc cgtgccccac atcatactga
      421 gcatatgaca agccggaacg tcgcattcgc ggtgaaataa gctgaccacc ttattaaaca
      481 ctttacctct tgtaattccg gggtcttcac tccaacgcgt atttatatac ggaaatccga
      541 cgatgaattc cactaaaaaa ggcgctacat ttaatttaat cttcgaccag ccgggcactc
      601 tcggcgtcaa agcggcgcta ttggcaatca cgctcgtaat attatcaacg gcttttccga
      661 ataagctcat cgtaaaggaa accgcgccga ggcagtggca gattacatgc agacgacgat
      721 tgccgatttg ctcgcgaatt ttggcgagcg ccgccggata gtcgaacaat gcaatatcgt
      781 ccatgttaaa gcgatgcgga gccaggttat aaggaagcct attgctcatg cggtaatcga
      841 gacaccagac atccgtaaaa ccgttatcga gcaggtagct caccagattg taatgctcgg
      901 gcatgatgaa catatccgac gaggtagtca gcccgtgaat tataagcacg acgtcatcgc
      961 atgcggcgcg attgaacctg agcatactca aacccaactt atcttccgtg ctgaaatagt
     1021 gcgtcgagac ctcggcgtct ttcacgcctt cgagggtgaa taacggaact gctcgcccgt
     1081 ttccgacggc atctttgccg ccttgcgcag ccgaaccggg cttgtctccg tttccgtttt
     1141 cggacggctg cggcactcgg cttatataaa tatcccacaa agtgccgaca aagaatttac
     1201 caaacgcgga taaagcctga gctcggtctg acagccgcgg agcatctacg cgaaaagtag
     1261 aaagctgctt gagaaaatca agcatataaa tgtgcatgat accggctgcg tagacctctg
     1321 cgcgagcctc ttcttcggcg cttacgtgcc cgcgaaagat ttttgtaaag agagtcgtcg
     1381 tatcttccca gacatcgaag cccgggtcat ctcgaatcaa tttatgcccg ctcagcgtaa
     1441 gcggaccttc ggcgtcgcga aagtaaagac gatagagcat gtgctttaca ctcgggtttt
     1501 cagtatcgac gaaaagattg aaaatccctt tttcaacctc cgaatctccc cagagagact
     1561 cgcagctgac gacgcccgcc gccgccgctt cgtgagcggc gtcggtaatg aagcggttga
     1621 cgtcgtcaat cgagattgtc agccgaaatt tgataaaagt ttctgcggat tctcccgttc
     1681 gataaccggc atcgtaatcg gtttcgcctt cggtgatgta acctttcatt tcttcggtaa
     1741 aactcaaaga agctttcgcg tcaataacag caggcttaga caaaatttct tcgcttttgc
     1801 tcttattcgt gtgtttgttc gttatttctt ttacttcaat catctgtttt gctctctttt
     1861 ctaattaatt ccgccgcaaa gcggtcggcg agcgccgcga tagtaaggga tggattagcg
     1921 ccgaccggac ccggcattac cgaaccgtcg gcgatataca aacccggaca gttaaaaacc
     1981 tcgccgaaag aattaaccac tcccatcgcc ggattaattc ccatcggaca gccgccgagc
     2041 gggtgaacgg ttatcgtcct gctcagatac attagcggat tctcctggaa ttttgcctgt
     2101 aggatgtcgg ctattttccg ggaagttttc gtcagttgct tgaaaagtgg gtttgagccg
     2161 cgtttgttcc aatcgacgtc cagatatttc tctttagcgt cgagcttcat attgccgtcc
     2221 gggctgtcaa gccccatccc gagcagcggt agcgaagcat acgaatattt actcgccgta
     2281 aaaacttccg caatttcggc gcttaaatcg ctgtcggtat ttaaaccgag cacaccgcag
     2341 atatatctgg aaagaaaacg cgcgtatcgc ttcagcgagt tgcccaaatc aacgctctga
     2401 ataacccagt ttaaaaattc aggaaaaccg gcgtcctgta tataaaatcc cggtccgctg
     2461 ccgccttcgg ttatatccgc gccgcgaatg gcgctggtta tcacgggacc gtaattggcg
     2521 ttgataacgc gaggaatttt tttgccgtcc gccgttattt tacttttcat cacaaaagta
     2581 agtaaatcgc cgttgccgca atagcgcgtg ccgagcgttt tgctgagcct tccgttaaag
     2641 gctttgcggt ttttcagcag caaataagtc gtgccgagcg ttccggcggc taatacgagt
     2701 ttgtcggagg taatcgtttt cgaaggcagc ttggcagtgt cgaattccct tccttcattt
     2761 tccggttcgt gaaccacgta atcaataacg taaccggctt cgtcaccggg tctgaaggag
     2821 cgaacctcgc accgggtgcg gatttctgca ccgagccgca ctgcttcgga caagtaagtg
     2881 tggtcgagcg tatttttact gccgtaatta cacccgatgt cgcattcgcc gcacaaacgg
     2941 caagtgtatc tttgaagttt gtggatattg tgagaatccg gcagcagcgg ttctgctaaa
     3001 gcgggctttt ggttttggtc ggcggcaaac atcaccgcca gattagggag catccattcg
     3061 atattttgag gattttgttt cattaactcc tcagccgcga atttcagagc cagagtttta
     3121 ggcgtcttat tataaggcga gaaatccatt ggatattttt gcggcttgag catcgcctcc
     3181 acgttatcat aatgaaaatc cagctcgtct ctgccgataa ggtctgcttc cgtaaaccag
     3241 gcggtgtcct tgcgcagtaa aacattggcg taaattaatg aaccgccgcc caatccgctc
     3301 gccaccagag cttccgagcc tctgaatgac cagacgttat aaagcccctg cagccctttg
     3361 ctcgggtccc aaaaattatt ttgcatcgcc agcgggcttc tcggaaaaga acccggagga
     3421 taagactttc cgcgttccag aacgcagact ttataaccgg ctttagccag ccggtaagcc
     3481 atgaccgagc cgccgaaacc ggaacctaca atgacaactt caaaatccat tgaataatct
     3541 ttagtaataa aatttaagtc ttttctgccg gagatttttt tgataagaaa tcaaataaat
     3601 tatggtttaa ttggtcgggg cttttgtttc gcgtcagcaa ataacgcggg gtttatcaga
     3661 aattccacgt ccgaaaatat tatcggaaac aggaaaagac tttttctaaa atgaaccgta
     3721 aagaatgaag aattatttct aattcttttt aataaaccgc aatttccgct gtaaagtgat
     3781 ggaatactaa ttgaaaatac cgcatttgtc aatacttttt cagtcgcctg ctgccattct
     3841 tattcgccgg gcaaatcggc ttcccgcgca aattaacctg aaaattcacg gtctaagttc
     3901 ttttctcatt tgtatatgat aaaagtccgc gaaaccttac aatgtttttt ttacaggcgg
     3961 aaaaccgcga aaaattattt gttttgcttt cgtttatgct cgcgcagaat ggcgtgcagc
     4021 atctcggagc ccaagccgtg cagcaacagg cgataaccgg ctcccgatgt cggcagcggc
     4081 acgagcggat gcttgttgtt caaaagaccg agatattgat tattgccgag caggcgcgat
     4141 acgtagagac ctaccgtttc gtcgagctgc agcgaatttg aagcgcgcca gaagctcgct
     4201 cccgacaaaa acagttgata aatcggcgtg tatatctcga tagccgattg aacgtccaga
     4261 aaattcgtcc agcgtttggg aaagcgctcc atcggaaact gcccgtccgt tatactgttg
     4321 aagtttaatt tttccggcgt ctcaatctgc ctgtccagtt cgcgcagctc gatattcaag
     4381 ttaataatga aattgaataa attcaaatcg tgcttgagaa aaatctcgtc cagaacgtct
     4441 tccagctttt ccggtcttaa aggattgagc ttgatttcaa cgtccgggac attttccgcg
     4501 ataaattcga gaatctccga accgatatga aaatgacgca gaattataaa attcgcttcg
     4561 ggactgacaa agtatttcag cccgatataa atcatccggt gcaggagttt cgacgacgtg
     4621 aataaattcg gaataattat gcgaaacagc gtaatggcgt gcaggctgag ccagagaaaa
     4681 ggcttaatca acggaaacag aaatctcgat ttcgagcgca tcgaaatcag cagcgcggct
     4741 tttgcttcat cgtcgatttg aatcgagcgg tctacataaa gcgccatcca cgggtccggg
     4801 tcagcgacat caaatttatt aatatcaaac tgcttggagt atttttccgg taacattaaa
     4861 ttatcctttt ttctttcctg cggcgggctg agtaatttcg attgaatttg ttcggcggtc
     4921 ggaagcttga tttcgcttgc cgcgccgatg aattcaaata agcacacgac tttccacatg
     4981 gcatcaaact gatttgccag aagtccgtgt ctggcgctgc tcggaaaaac gtggtgattg
     5041 gcgtgccagc cttcgccgag agttaaaatt ccgagcaggt ggttattgcg cgcgaaatga
     5101 tttgcctgaa aaggtcgttt cccgtaaaga tgtgcaacgc tgtcaatcgc atctccgaga
     5161 ttgtaaataa ttacggtcgt aatccaaagc agaataaaac cgacccgccc ccagacgaaa
     5221 taagaaatcc cgtagaaaac cagcgcgtgc agccagcagg cgagcgcgaa aaacgccggg
     5281 cggctgacga aacggtaata accgtcgccc gcgacatccg ccgccaaacg attatgctcc
     5341 tgattggtgc gagggcgatt ccagccgtcg tatatcgtcc gcagcggtcc cgcgattgaa
     5401 tagagaaaac aagccagcgg gtttttcgtt ccgatatacc agccgacgtg agaatgccag
     5461 aatccgtccg tgacgggcga atgcgggtct gattcgacat cggtatggcg gtgatgaaaa
     5521 cggtgatttc ccacccattg aacgggcgtt ccggcgggca gaccgagcgt gaccagaact
     5581 ctctccagcg gtttccataa acgaagagag cgatggctca aacatctgtg ataagcgata
     5641 ttgatagcca gcccggcagc gcagtagtaa ccgaaacaat aagcgaaaag ggtcgaataa
     5701 taataaaaat ttgagctttt catcaatggc ggcgtttaaa aatttcctat ctcttccaat
     5761 tgcagcgcgt aaagccgcgc cgtgactttt gctgttttga cgattctttc gaccaattcc
     5821 gcgtttaaaa ttccgcttgc cagagcttgt tcaagttttt ccaggtgatt ttcgtcgttc
     5881 gcgccgtgat acagcaaaaa cgaaacctgt gcggcttgca gattcagttg ctcgcgaatc
     5941 cggttgcccc atttattcgc cattttttgt cccaagccct cgatgataaa catcgcgccg
     6001 agcagctcga acggattttc ccggctcgag caatgaaaca tccatgccga caaagcttct
     6061 gagccgatat ttttttcggc tttctggatt tcctccaagc tgccgccgac cgaaacaaag
     6121 tcgcgttcga gcatttgaaa atcccggtgt tcttccttcg cgtgaccgat aaataaagag
     6181 cgtagcggaa aagcttctgc cgtaatattt gaagcggcgc gtgaaatcca gcgcccgccc
     6241 tcgacaactt gttggcgtaa attcagcaaa agattgcggt aatcttcaat acgaagcttg
     6301 ccgcgattca gacgctctat tatcggaatt tgattgagac ggctttcaaa atccagccag
     6361 acatcggtta attttctcca taaagtgctc tgaagctctg ctttttcggc ataatgctta
     6421 ttcttatcga caaaaggcga aattgaatgc tgctctgcgg cggcagcgtc aatcacgtcg
     6481 gcttcgccta aaatttcgtt ttgacgttca gccgccgtct cgacgacggt caacatcata
     6541 aacgagacca caaaacgccc gctttccgga accatacaga gaattttttg accgggctgc
     6601 aattttcccg agccgaaaag ctcttccagc ataataaata tcgaagccgc gccggtattt
     6661 cctttagtgt agagattggt aaaccaacgc tcttcggaaa tcatgccatt tcctttctcc
     6721 agtagttcaa taatcttaga acgaaaaacg tgcgaagaat aatgacagac aaaccagtct
     6781 atgtcttttg aatttatacg tccctgctcg ataagctcga aataacgctt gactccgagt
     6841 tttaagacgt tttccaaaat tttcacatct tgtttcagaa gaagtgcgcc gtcgcgcgcc
     6901 gcggcggcga aattcggata atcataccag gatttttcca gcgtgccgtc agcctgttta
     6961 tttgctcccg aatacatgca aacatcgaaa ctgctgccat atgatattaa atctatccat
     7021 tctattttga gagaaactcc ctttctatta ggacggtttt ccagaacggc tgcgccggct
     7081 ccgtccgaca gcatccagcg caaaaattcg gcgtcaaaag gcagctttcc gtttttctga
     7141 aaacttgccg tttcctcaaa caaaccagga cgaaagaatc gactcgtaaa ttcgctgccg
     7201 cagaccacgg aattctctct gactcccgac aaaatcccgg taaaagccgc ttgcagagcc
     7261 attatgctgc ccgcgcagat gctttgaaag ctcgcgattt cgcaaggcgg cagacggctg
     7321 gcgccgtgga ctaagctggc gaagcccgga gccagcaaat cgctctgcga ggtggacgcc
     7381 gccaaaaatt gaacgtcctt cgcgtctatc tccgattgct tcagggaatc gatgacggca
     7441 ttcgccgcca gttcataatt ccagaatccg actttgccgc cggcatcaat tgcgtaatgt
     7501 ctggtgcgga ttccgttctg ccttagaata cggcttttga ggcgtgactg tctgccgccg
     7561 attaagccca gataatcctc gatttcattg tttgcgacgg gctcgcccgg aagaaactta
     7621 ccgaacgagg taatataaac cgaatttttt ggtaacatat tgaacttcaa aaggtttgca
     7681 gtaaaaataa ccctgagggc atagcgggtg taacgaacgc tttccagcaa taatacaaga
     7741 tgaatatttc tgccacagaa tgttgagttt agaaaaatta tcgccgaaac tccggcttgg
     7801 aatataaaag tatttgcggt tagcgactct atcaaataga ctctgcactg tcaagatttt
     7861 taaacaccag atagtgtccc ggtctaatct ttttcaaatc ttaatcggtg cgcaaaaagc
     7921 catgaaattg tgaagtgtta aacctaaatt ttataagcct ctaatagatt taattctcgc
     7981 cgagcaaaat ctctggtttt tgcacaggtc ggaattgaat gaatattgaa aacattcaaa
     8041 gccattctct ttaccgggaa tggttttgaa tgtttgctgt tttatttgcc atttcaatta
     8101 tccatcacaa aaactattga cactcatcag gaaatcaact agagtcactt ttaaccgacg
     8161 accaattaaa tccattagca ttcgagggga aatgctaaaa ccggattatt tgcagatttt
     8221 gttatttgca gattttcggg tgcggattta gggcgtcgtt ttttgtttga aataactctc
     8281 gacccaggta aatcttcacc attttgagat cgtttaagcg aaatgatg
//
LOCUS       DASWNE010000896         4905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_134469, whole genome shotgun sequence.
ACCESSION   DASWNE010000896 DASWNE010000000
VERSION     DASWNE010000896.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4905
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134469"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VGB68_17565"
     CDS             <1..365
                     /locus_tag="VGB68_17565"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY0051106.1"
                     /translation="NNSSGGQIMPAPEEPVRDALPRVRAGEKPIAKAVVNSAAISLPK
                     PEYPAAAKAVRAAGTVQIQVTIDEQGNVISVKAVSGHPFLQMAAVKAARQAKFRPTLL
                     SGTPVKVTGIIVYNFVAQ"
     gene            378..908
                     /locus_tag="VGB68_17570"
     CDS             378..908
                     /locus_tag="VGB68_17570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEY0051107.1"
                     /translation="MATAVPTDFTNQVEYTEEVADFSPEVVEEMRSHLVKYPPERKRS
                     ALIPLLFVIQRERGYVDNPGVNFLAKFLEIEVTDVWETVTFYSMFNLRPVGRHHIQIC
                     KTLSCRIMGEPEITEHICHRLGIHAGETTEDKKFTVSLVECLGSCGTAPMMQIGFDYY
                     EDLTIGKVDKILDSLE"
     gene            988..2316
                     /gene="nuoF"
                     /locus_tag="VGB68_17575"
     CDS             988..2316
                     /gene="nuoF"
                     /locus_tag="VGB68_17575"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEY0051108.1"
                     /translation="MEIKAIGCEPLQLKRMHIKDGHTLKVYQETGGYESLKKAMNMSP
                     DEIINEVKASALRGRGGAGFPTGMKWSFVPRNSPKPKYVVCNADESEPGTFKDRYLME
                     RDPHSLIEGMIIAAFALGATAGYIYTRGEYKYLIDIMDVALAEARQAGFLGKNILGSD
                     FSHEIYTHTGAGAYICGEETALLSSLEGYRGHPRMKPPFPAVEGVYACPTVVNNVETL
                     TAVPQIIEMGGTAWRDLGTEKSGGTKLWSVSGHVKKPGVYELPMGYKDMEKFIMEDCG
                     GMLRADKKLKAVIPGGSSVYIMNAEQIIGKNVTLDYEGLVAGGSMVGSGGFIVMDESV
                     DVMESTKNLTEFYKHESCGWCTPCREGTDWLVKIFNRISDGGGRPEDAQLLLDICDNI
                     EGKSFCPLGDAAAWPIQSAIKRFPEDFRKWLVDKANAGGNGDARLATAKV"
     gene            2496..4802
                     /locus_tag="VGB68_17580"
     CDS             2496..4802
                     /locus_tag="VGB68_17580"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY0051109.1"
                     /translation="MSQEQIKVTINGQEFEVEKGAQLIEVCREKGFGIPSFCYYSDLA
                     LQASCRMCLVRIEKMPKLQTSCTITCTDGMVVTTQSEEIEKAQRAMGEFLLANHPLDC
                     PVCDRGGECELQEMIFDWGDLEERFTERKNATPEKFLSPIVANDPQRCILCKRCTRVC
                     DEWMGEEAIEAGNRGVNTVIGTYGGWLNCSQCGNCIEVCPTGTLLDGVYRHETRPWEM
                     DQTISTDVYGSDGMQLSIGSRGGEIHRIVARDRYVNGLNGEFLDVKARFAHGFINHPD
                     RIKTPLIRYKKGGRLIPATWDDAVGFVANKLRESGNNVGVVASSRLTNESIFTLKRFA
                     TEVVKTDNYAVSDGYDLAPFFDNLSAPLCTHKEIRHASTILLIGGDPEEEQTFTAKQM
                     RQAIRNGGAKLIVVHHTPLRITEKAAHFLHVNPDSLDAFALAFADAATAEIATKLGIE
                     KSQIDDMLKTIGETEGDFIVMFGGDLSAEAQIVLANSVGNYASDTRRALLHPLAAYNN
                     SVGAHDMMAGRNSLEEVLRNSRALLIAGSLSDENAGLLADKDFVVVQELFETATTEYA
                     DVVLPAASFAESDGTFTNNTGFVQRVRKAIEPLHQSRTDWMITARIAREMDVDFGYGF
                     GSTAVFKSIADTVAPYAGLRYPDLKDESKPVQVKHEIQAKRDLSNEIDVLNRRVESMP
                     DTAEKYTETPRIGYKLHRVTTMTGKTPQFHLLAHGNPKPENLLVSPLVQFNLDGTPKI
                     EEMAEAAAVGVKDRLDDKETVPNTVVLR"
BASE COUNT         1268 a         1228 c         1381 g         1028 t
ORIGIN      
        1 aaaataattc ttccggcggg caaataatgc ctgctccgga agagcccgtg cgcgacgctt
       61 tgccgagagt gcgagccggc gagaaaccga tagctaaagc cgtcgtcaac agtgccgcga
      121 taagtttacc gaaacccgaa tatccggcgg cggcaaaagc ggttcgcgcg gcggggacgg
      181 ttcagattca agttaccatt gacgaacagg gaaacgtaat ttcggtaaaa gccgtctccg
      241 ggcatccgtt tctgcaaatg gcagccgtca aggcggcgcg gcaggctaaa ttcagaccga
      301 cactgctttc gggaacgccg gtcaaagtta cgggaattat cgtttataat tttgtcgcac
      361 aataaatttt agaaaatatg gcaacagcag ttccgaccga ttttaccaat caggtcgaat
      421 ataccgaaga agtggcggat ttttcgcccg aagtcgtcga ggaaatgcgc tcgcatctcg
      481 tgaaatatcc gccggaaaga aagcgttcgg cgctgattcc gcttcttttc gtcattcagc
      541 gcgagcgcgg ctacgtcgat aatccgggcg tgaattttct ggcgaagttt ctggaaatcg
      601 aagtgacgga cgtttgggaa accgtgacgt tttattcgat gttcaacttg cgtccggtcg
      661 ggcgtcatca catccaaatc tgcaaaacgc tttcgtgcag gattatgggc gagccggaaa
      721 tcaccgagca tatttgtcac aggctcggca tccacgccgg agaaacgact gaagataaaa
      781 agtttacggt ctcgctcgtc gaatgtctcg gaagctgcgg aaccgcgccg atgatgcaga
      841 tcggttttga ttattacgag gatttgacga tcggcaaagt cgataaaatt cttgattcgc
      901 tcgagtaaaa tgcgagcttt tatcgctcgg gcaatttttg ttttccgttg tccggcaatc
      961 ggaaaagttg taaagatttt ttagtttatg gaaatcaaag caatcggatg cgagcctcta
     1021 cagctcaagc gtatgcacat caaagacggg catacgctaa aggtttatca ggagaccggc
     1081 ggctatgaat cgctgaaaaa agcgatgaat atgtcgccgg acgagattat taacgaagtc
     1141 aaagcgtcgg ctttgcgcgg gcgcgggggc gcgggttttc cgaccggaat gaaatggagc
     1201 ttcgttccgc gcaattcgcc gaagccgaaa tacgtcgtct gcaacgccga cgaatcagag
     1261 ccgggcacgt tcaaagaccg ttatctgatg gagcgcgacc cgcattcgct gattgaagga
     1321 atgataatcg cggctttcgc gctcggcgcg accgccggat acatttacac acgcggcgaa
     1381 tacaaatatc tgattgatat tatggacgtt gctctagccg aagcgcgcca agccggtttc
     1441 ttaggcaaaa atattttagg ctcggatttt tcgcacgaaa tctacacgca caccggcgcg
     1501 ggcgcgtata tctgcggcga ggaaaccgcg cttttatcga gcctggaagg ctatcgcggg
     1561 cacccgcgta tgaagccgcc gtttccggca gtcgaaggcg tctacgcctg cccgacggtc
     1621 gtcaataatg tcgaaacgct gacggcggtt ccgcagatta tcgaaatggg cgggacggct
     1681 tggcgcgatt tgggcacgga aaaatcgggc ggcacaaagc tctggtcggt ctccggtcac
     1741 gtcaaaaaac cgggcgttta cgagcttccg atgggctata aagatatgga aaagttcatc
     1801 atggaagact gcggcggaat gcttcgggcg gacaaaaaac tgaaagcggt cattcccggc
     1861 ggctccagcg tttatattat gaacgccgaa cagattatcg gcaaaaatgt tacgcttgat
     1921 tatgaaggtt tagtggcggg cggctcgatg gtcggctcgg gcggtttcat cgtgatggac
     1981 gagagcgtag acgttatgga atcgacgaaa aacctgactg aattttataa gcacgaatcc
     2041 tgcggctggt gcacgccgtg ccgcgaagga accgattggc tggtgaaaat tttcaatcgt
     2101 atttccgatg gcggcgggcg accggaagac gcgcagctgc tgctcgatat ttgcgataac
     2161 atcgaaggaa aatcgttctg cccgcttggc gacgcagcgg cgtggcctat ccaaagcgcc
     2221 attaaacgat ttccggaaga ctttagaaaa tggcttgtcg ataaagcgaa cgccggcggc
     2281 aacggagatg caagacttgc gacggcgaaa gtttaattaa ttaaagggag aaaagaaaaa
     2341 tgggaaaagg cgataaaaga acacgacgcg gaaaaatttt tgccggcagc ttcggcaaat
     2401 cgcgtccgaa accgaaagtt aaaacagcga ataccaataa agaacataaa accgaacaaa
     2461 aagagtcata aaatctttta ttgaaattat ttaagatgtc tcaagaacag ataaaagtta
     2521 cgattaacgg acaggaattc gaagtcgaaa aaggcgcgca gctgattgaa gtgtgccgcg
     2581 agaaaggctt cggaattccc tcgttctgct attacagcga ccttgctttg caggcatcgt
     2641 gccggatgtg cctggtgcgc atcgaaaaaa tgccgaagct gcaaacgtcg tgcacgatta
     2701 cctgcaccga cggaatggtc gtcaccacac aaagcgaaga aatcgaaaaa gcgcagcgcg
     2761 cgatgggcga atttctgctg gcgaatcacc cgctggattg tcccgtctgc gaccgcggcg
     2821 gcgaatgcga attgcaggag atgattttcg actggggcga tttggaagag cgctttaccg
     2881 aacggaaaaa cgccacgccg gaaaaattcc tgtcgccgat tgtcgccaac gacccgcagc
     2941 gctgtatttt atgtaaacgc tgcacgcgcg tctgcgacga gtggatgggc gaagaagcca
     3001 tcgaagccgg aaatcgcggc gtcaacacgg tcatcggaac ttacggcggc tggctgaact
     3061 gctcgcagtg cggaaactgt atcgaggtct gcccgacggg aacgctgctc gacggcgttt
     3121 accggcacga aacgcgaccg tgggaaatgg atcaaaccat ctcgaccgac gtttacggct
     3181 cggacggaat gcagctttca atcggctcgc gcggcggcga gattcatcga attgtcgccc
     3241 gcgaccgtta cgtcaacggc ttgaacggcg agtttctcga cgtcaaagcg cgtttcgcgc
     3301 acggatttat caatcatccc gaccgcatca aaacgcctct gattcgttac aaaaaaggcg
     3361 gcagattgat tcccgcgacg tgggatgacg cggtcggttt tgtggcgaac aagctcagag
     3421 aaagcggaaa caacgtcggc gtcgtcgcgt cgtcgcgcct gacgaacgag tcgattttta
     3481 cgctcaaacg ttttgcgacg gaagtcgtca aaacggacaa ttacgccgtt tccgacggtt
     3541 acgatttagc gccgtttttc gataatctga gcgcgccgct ctgcacgcac aaagaaattc
     3601 gacacgcctc gacgattctg ctcatcggcg gcgacccgga agaggaacag acctttacgg
     3661 cgaaacagat gcgacaggct atccgcaacg gcggcgcaaa attgattgtc gtgcatcaca
     3721 cgccgcttcg cattaccgaa aaagccgctc attttctcca cgtcaatccg gattcactgg
     3781 acgcttttgc attggctttt gccgacgcgg cgaccgcgga aatagcgacg aagctcggca
     3841 tcgagaaatc gcagattgac gatatgctga agacaatcgg cgaaaccgaa ggcgatttta
     3901 tcgtgatgtt cggcggcgat ttatcggcgg aagcgcagat tgtgctggca aattcggttg
     3961 gcaattacgc ttccgacacg cgccgcgcgc tgcttcaccc gctcgccgct tacaacaact
     4021 cggtcggcgc gcacgatatg atggctggaa gaaattcgct ggaagaggtt cttcgaaatt
     4081 cgcgggcgct gctcatcgcc ggaagccttt cggacgaaaa cgccggactg cttgccgata
     4141 aagatttcgt cgtcgtccag gaattatttg aaacggcgac gaccgaatac gccgacgtgg
     4201 ttctgccggc ggcgtcattt gccgaatccg acggaacttt tacgaacaac accggatttg
     4261 ttcagcgcgt gcgcaaggca atcgagccgc ttcatcaatc gagaaccgac tggatgatta
     4321 cggcgcggat cgcgcgcgaa atggatgtcg atttcggcta cggcttcggc tcgaccgccg
     4381 ttttcaaatc aatcgccgat acggtcgcgc cttacgcggg cttgcgttac ccggatttaa
     4441 aagacgaatc gaaaccggtg caggtcaaac acgaaattca agcgaagcgc gatttgtcga
     4501 atgaaatcga cgttttgaac cggcgcgtcg aatcgatgcc cgacacggcg gaaaaataca
     4561 cggaaactcc gcgaatcggg tacaaactgc accgcgtgac gacgatgacc ggcaaaacgc
     4621 cgcagtttca cctgctggcg cacggcaatc cgaaaccgga aaacctgctg gtttcgccgc
     4681 tcgtgcagtt taatctggac ggcacgccga aaatcgaaga aatggcggaa gcggcggcgg
     4741 tcggcgtcaa agaccgtctg gacgataagg aaaccgtgcc gaacacagtc gttctcagat
     4801 aatttgcggg atcgctttac gccgggcgcg ataacagttt aaatttaaag ttcgcaaaat
     4861 ttaaaatacc ggcggcacgc gccgcacttc ggcaggcttt aaaaa
//
LOCUS       DASWNE010000897         2563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_65560, whole genome shotgun sequence.
ACCESSION   DASWNE010000897 DASWNE010000000
VERSION     DASWNE010000897.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65560"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="VGB68_17585"
     CDS             <1..701
                     /locus_tag="VGB68_17585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051110.1"
                     /translation="FIVGATVNITNDSSVKPLKDEKALKIIDEYIETIGGRENLKKLN
                     SLAASGKVEMKQAGTIVEGEVEIHRKFPNKIVKVLKIESLGEIREVFDGEKSFIQTDF
                     MGTQKMEIRTNEINLAANFRELLDAREIYQSITFESAFENKGKKINLVKAVSKQGTSV
                     YFAFDAATKLLISRAGTSVSAYYDDYRQVGEWLFPFRISESIMTYKLKEIKPNAPVDD
                     NRFVEKESCFTRVD"
     gene            821..2140
                     /locus_tag="VGB68_17590"
     CDS             821..2140
                     /locus_tag="VGB68_17590"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY0051111.1"
                     /translation="MNKKTIRDIDIKGKRVFIRVDFNVPIKDGRIEDDTRIRGALPTI
                     KYAVEQGAKVIVASHLGRPLKDKKKAEEKGMPYDINSYSLNPVYEYLRALPELQSVSK
                     SGSDEPVVYANEQAAALGKAEVKNDKIFFAGDCVGDVVRAQVGKLKEGEVLLLENLRF
                     YEGEEKNDEEFARALAEYADVYVNDAFGAAHRAHASTAGITKFVPAAVAGLLMEKELN
                     FLSKALHNPERPFVAILGGAKVSDKIPVIESLIERKVDKLLIGGAMAYTFFKAKGYTI
                     GKSLVENEMLETALEIEQKAKDAGVELILPTDHQVVDSYDPLNSRKTIPVEFTNKGLV
                     GLDIGIETVAIFKQALEGAKTIVWNGPMGMFEEKPFDEGTVAIAQAVADATDKGATSI
                     VGGGDSVSAVNQAGLDEKISHISTGGGATLEFLAGDELPGVAALNDK"
     gene            2374..>2563
                     /locus_tag="VGB68_17595"
     CDS             2374..>2563
                     /locus_tag="VGB68_17595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051112.1"
                     /translation="MAVTKSRRALVAIINVQDHAPRNVRWLYRFIDASGAIASDLLAI
                     NYGKYEKLYGKNATGKRFL"
BASE COUNT          747 a          618 c          680 g          518 t
ORIGIN      
        1 gctttatcgt cggcgcgacg gtaaatatca cgaacgattc gagcgtcaag cccttgaaag
       61 acgaaaaagc tctgaaaata attgacgaat acatcgaaac catcggcggc agggaaaatc
      121 tgaaaaagct gaattcgctc gcggcaagcg gcaaagtgga aatgaaacaa gccggaacga
      181 tagtcgaagg cgaagtggaa attcaccgca agtttccgaa caaaatcgtc aaagtcctga
      241 aaatcgaatc gctgggcgaa atcagagaag ttttcgacgg cgagaaatcc tttatccaga
      301 ccgattttat gggcacgcag aaaatggaaa tccggacgaa tgaaataaat ctggcggcaa
      361 atttccgaga gcttctggac gcgcgcgaaa tttatcaatc aatcacgttc gagtccgctt
      421 ttgaaaataa aggaaagaaa atcaatctcg tcaaagccgt ctccaaacag ggaacgagcg
      481 tctattttgc tttcgacgcc gcgacgaagc ttttaatcag ccgcgccgga acgtccgttt
      541 ccgcttatta cgacgattac cgccaagtcg gcgaatggct tttcccgttc cggatttcgg
      601 aaagcattat gacttacaaa ttaaaggaaa ttaagccgaa cgcgccggtc gacgacaacc
      661 gctttgtcga aaaggaaagc tgttttacca gagtcgattg agcaggcagc tttaaaaagt
      721 ggtaaacgat aaaagataaa cggcgagcga acgtaaaaac ttgccgttta gcctttatcg
      781 tttaccactt tccgtgtctt tgcgttaaaa tttttgcatt atgaacaaga aaacaatcag
      841 agacatcgac atcaagggca aaagagtttt cattcgcgtt gatttcaacg tgccgataaa
      901 ggacggacgg atagaggacg acacgcgcat cagaggcgcg ctgccgacga taaaatacgc
      961 cgtcgaacag ggcgcgaaag ttatcgtcgc gtcgcatctg ggcagacctc tgaaggacaa
     1021 aaagaaagcc gaagaaaagg gaatgccgta tgacataaat tcatactcgc tcaatccggt
     1081 ttacgaatac ctgcgcgctc tgcccgaatt gcagagcgtt tcaaagagcg gcagcgacga
     1141 gccggtcgtc tacgcgaacg agcaggcagc ggcgctcggc aaagccgaag tcaaaaacga
     1201 taaaattttc ttcgccggcg actgcgtcgg cgacgtggtg cgggcacagg tcggaaaatt
     1261 aaaggaaggc gaagttctcc tgctcgaaaa tttgcgtttt tacgaaggcg aagaaaaaaa
     1321 cgacgaagag tttgcgcggg cattagccga atacgccgac gtctacgtca acgacgcttt
     1381 cggcgcggcg caccgcgcgc acgcttcgac cgccggaatc acgaaattcg tcccggcggc
     1441 ggtcgccgga cttttgatgg aaaaggaatt aaactttctg tcgaaagccc tgcacaatcc
     1501 cgaacgcccg tttgtcgcta tcctgggcgg cgcgaaagtc tccgacaaaa ttcccgtcat
     1561 cgaatctttg attgagcgca aagtcgataa attattaatc ggcggcgcga tggcttacac
     1621 gtttttcaag gcgaaaggct acaccatcgg caaatctctg gtcgaaaacg aaatgctcga
     1681 aacggcgctg gaaatcgagc aaaaagcaaa ggacgcgggc gtcgagctaa tcctgccgac
     1741 cgaccatcag gtcgtcgatt catacgaccc gctcaattcg cgcaaaacga ttccggtcga
     1801 attcaccaac aaaggcttgg tcggtctgga catcggcatc gaaacggtcg ccatcttcaa
     1861 acaagctctg gaaggcgcga aaaccatcgt ctggaacgga ccgatgggaa tgttcgagga
     1921 aaaaccgttt gacgaaggca cggtcgccat cgctcaagcc gtcgccgacg ccaccgataa
     1981 aggcgcgact tcaatcgtcg gcggcggcga ttcggtctcg gctgtcaatc aagccggttt
     2041 agacgaaaag atttctcata tttcaacggg cggcggcgca actctggaat ttctggcggg
     2101 cgacgaactg cccggcgtcg cggctttgaa cgataagtaa acagattaaa gctcaatacg
     2161 gcgagagctc agaccagctt caggcgctcg ggctgaaaaa gaaatccgaa tataaaagtc
     2221 cgggcgaaaa aacgtcggaa ccgccgacgc cgtaaggctc ggaaaatgcg gaacgcaaag
     2281 cgcagagtgc aaaatgaatc cgctttcttt attttgcatt gcgcgcttat taagggttat
     2341 atatttaccg cgttcaaaca gaaagaaaaa tttatggcgg tgacgaaaag caggcgcgcg
     2401 ctggtggcga taataaacgt tcaggatcac gcgccgcgaa atgtgcggtg gctttaccgg
     2461 tttatcgacg cttcgggagc gattgcttcg gatttgctgg cgattaatta cgggaaatac
     2521 gagaagctct acgggaaaaa tgcgaccgga aagcggtttc tcg
//
LOCUS       DASWNE010000898         1670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_666154, whole genome shotgun sequence.
ACCESSION   DASWNE010000898 DASWNE010000000
VERSION     DASWNE010000898.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666154"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="VGB68_17600"
     CDS             <1..877
                     /locus_tag="VGB68_17600"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEY0051113.1"
                     /translation="TDVKLLELSNADLPVLGQLALRAPGTNQHSHAVGQLAEEACRAV
                     GANALLARIGALYHDIGKTAAPEHFVENQLGKNPHDRLKPAQSAKIIIAHVNYGIKLG
                     KEIGLPARIIDFIPQHHGTRTLHYFLRKAQAQAGENQEVEESDFRYPGPKPQFKEAAI
                     MMIADSCEAAARSLSQPTPENIRYIITKILDAILSDDQLDECDLTLRELTLIRESMIK
                     SLVAIYHSRIDYPGYTPPASFAKLIVPADEDSEERGLKYSNPAEIPVSRGGEVEDEAV
                     DRTEQPDKAEAKTVK"
     gene            1200..>1670
                     /locus_tag="VGB68_17605"
     CDS             1200..>1670
                     /locus_tag="VGB68_17605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051114.1"
                     /translation="MITVELDIFSGNPNPVWTLTAQEEKELIARVVAEPSLTLPPKSA
                     GGLGYRGFIISAGKEARNTLEKSGLPSRFYIESNENADAEAALLNSIEAGKRVADDVR
                     QVALESIRATNNIWSEYWRTHTTSDSLALDIPTAESYEPSVNSLNANIPLDAARP"
BASE COUNT          443 a          470 c          407 g          350 t
ORIGIN      
        1 gaccgacgtc aaactgcttg agctttccaa cgccgatttg cccgttctcg gacaactggc
       61 gctgcgcgcg cccggaacga atcagcattc gcacgccgtc ggacagcttg ccgaagaagc
      121 ctgccgcgcg gtcggcgcca acgctctttt agccagaatc ggcgcgcttt atcacgacat
      181 cggaaaaaca gcagcgccgg aacatttcgt cgaaaaccag ctcggcaaaa atccgcacga
      241 ccgcttaaaa ccggcacaaa gcgctaaaat catcatcgcc cacgtcaact acggcatcaa
      301 gctcggcaag gaaatcggct tgcccgcgcg tatcatcgac ttcatcccgc aacaccacgg
      361 cacgcgcacg ctgcattatt ttctgcgcaa agcacaggcg caggctggcg aaaatcagga
      421 agtcgaagaa tcggattttc gctatccggg accgaagccg cagtttaaag aagccgcgat
      481 tatgatgatt gccgacagct gcgaagccgc ggcgcgctcg ctttctcaac cgacgccgga
      541 aaacattcgc tacatcatca ccaaaatcct cgacgcaatc ctcagcgacg accagctcga
      601 cgaatgcgat ttgacgctgc gcgagctgac cttaatccgc gaatcaatga ttaaatctct
      661 cgtcgccatc tatcattcgc gcatcgatta tccgggctac acgccgcccg cttcattcgc
      721 caagctcatc gttccggcag acgaagattc ggaagaacgc ggcttaaaat actcgaatcc
      781 ggcggaaatc ccggtcagcc gcggcggcga agtcgaggac gaagccgtcg accgtacgga
      841 gcagcccgat aaagccgaag ccaagacggt gaaataattt tttacaaatc gatttttaca
      901 aatcgatttt aacgcaagtc aaatgattcg ttgatttcac tattaaaagc gagcgtaaat
      961 gcccggcatc ggaaaaccag cctgccgccg gcggcttgca ttttgttttg ccggatgtta
     1021 ttacagtgat aacaacaaac atgtggcggt tatcgcttga atcggatgcg gcgatagaaa
     1081 aaccgcttag cccagcatcc tctcaaatca taatttggga ggttaaacct gaaaaagccc
     1141 atagcgcctt tctctttttc ccttccaaaa caatcacaat cgcttgcaca ggaggattta
     1201 tgattactgt cgaactagac attttttcgg gaaatccaaa tcccgtctgg actttaaccg
     1261 cgcaggaaga aaaagagtta attgctcgcg tcgtcgccga gccgtcttta acgcttccgc
     1321 cgaagagcgc cggcggactg ggttatagag gatttatcat ttccgcaggc aaagaagcga
     1381 gaaacacgct cgaaaaatcc ggtctgccct caaggtttta cattgaatcg aacgaaaacg
     1441 ccgatgcgga agccgccctg ctcaattcca tcgaagccgg aaaaagggta gctgacgatg
     1501 tgcggcaggt tgctctggag tcaatcaggg cgactaataa tatttggtct gagtattggc
     1561 ggacgcatac cacgagcgat tcgctggctt tagacattcc gaccgccgaa agttacgagc
     1621 cttcggttaa ttctttaaac gcgaacattc cgctcgatgc ggcgcgaccg
//
LOCUS       DASWNE010000899         3775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_699687, whole genome shotgun sequence.
ACCESSION   DASWNE010000899 DASWNE010000000
VERSION     DASWNE010000899.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699687"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2226)
                     /locus_tag="VGB68_17610"
     CDS             complement(<1..2226)
                     /locus_tag="VGB68_17610"
                     /inference="COORDINATES: protein motif:HMM:NF018470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein IcmF/TssM N-terminal
                     domain-containing protein"
                     /protein_id="HEY0051115.1"
                     /translation="MTAWYGNQLKFALGLGGLFSFYGVVAMVVWVAGTKMNYPVNSRI
                     VIIALILLTLPVALIIGYLASRRKAKKEAAKTDEAAGAKAEQKTDAVKPQKLAAPDTN
                     GDLTKSAEEVAQFLKSSNLDVYSLPWYLVMGTPKAGKSSLVLGSNLNFQNLPSQRQSE
                     QKFIRPTRGVDWRVTSEAVFVDTAGRYQTEGLDGEEWSSLLETIKKYRGARPLDGMIL
                     SVSAERVLHADEKEIEEQAKVLRARIDEAVQRTKVRFPIYLVFTHADAIEGFRDSFSN
                     SQKEGQNLVWGATIPLENADNAHSLFDAEYELLQNSIMKRRLIRLSAPFPPVRQLRIF
                     NFPLHFGSARRKLGAFVSTLFRPNPFSESPFLRGFYFTAVPVTRPKMPQGGQTLAGIP
                     QTIGQTYFTEKLFREVVLRDKDLVATLQAQRQKPPILGWLATITGAFLVALLLGLSAY
                     SLYQNKKLLDEASANGEKVLTLYKSDAGRDPFTKTPDEALVELNTIENLRKNLVDFDE
                     YERSGAPWLMRMGLYSGDGIYRERLLKIYFNAVEQRFKAPVVKRLEEDLRKFTESQNV
                     ANPAALTQAEEEALGKNYDLLKAYLMLSGEPKYREHAEASTIVNSLAGYWNTESKLGT
                     GLEMASQQQLEFWAKQVDREEFPFIKLKDNLVTGARKKLQAFPAWQRYYKRKTTDISK
                     ELNAKNGDLTVANILAKEGADASFIEGGYRVPNAFTMEGVELMDAAVHNAEQELRADE
                     WV"
     gene            complement(2305..3018)
                     /gene="icmH"
                     /locus_tag="VGB68_17615"
     CDS             complement(2305..3018)
                     /gene="icmH"
                     /locus_tag="VGB68_17615"
                     /inference="COORDINATES: protein motif:HMM:TIGR03349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HEY0051116.1"
                     /translation="MSEQRAQSSRRSQQQQQNANDLVTFAAPVFDLILRLQAGIVKPS
                     NELRPKIKSLLDDFEQRAERYRFNHKVVNVAKFALASFVDETVLTGDFHLKEEWEKYP
                     LQLEYFGEQLAGNKFFEKLQAMIAQIEQTADAVEIYYVCMLLGFKGRYAVYEHEKLHS
                     IMQQTADALVKAGKITKVELSPHWQANDQPKPPEKRGMPTWAKVAAFGGLAFGLLLYL
                     VMFLLSSKFLDDAMQKLQL"
     gene            complement(3077..>3775)
                     /gene="tssK"
                     /locus_tag="VGB68_17620"
     CDS             complement(3077..>3775)
                     /gene="tssK"
                     /locus_tag="VGB68_17620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HEY0051117.1"
                     /translation="SLGEQRRQRNASLADFTTSEVAVFWLLHTINSSIPTMAHYFRSP
                     LLHPEKLYLEMSQIVGQLMTFSTDLHPKDIVKYDHDDLHFTFLNLSVQLRDLLETVIP
                     SRCVPIPLEKINPTLYVGRVEDERLLKEAGFFLAVRAQMPEAKIIEDVPRVIKIASRD
                     VIDTVVGRALPGVNLTHSNPPPAPIPTRVGFRYFQLESSGAYWEGIKGSKIIAVYVPE
                     KIPDEKLEMYAVKP"
BASE COUNT          845 a         1042 c          971 g          917 t
ORIGIN      
        1 cacccactcg tcggcgcgca attcctgctc ggcgttgtgg accgccgcgt ccatcagttc
       61 cacgccttcc atcgtgaatg cgttcggcac gcggtagccg ccttcgataa agcttgcgtc
      121 cgcgccttct ttcgccagaa tattggcgac ggtcaaatcg ccatttttcg cgttcagctc
      181 tttggaaatg tcggtcgttt tgcgcttgta ataacgctgc cacgccggaa acgcctgcag
      241 ttttttgcgc gcgccggtga cgagattgtc tttcagcttg ataaacggaa attcttcgcg
      301 gtcaacctgt ttcgcccaga attcgagctg ctgctgcgac gccatctcca agcccgtgcc
      361 gagcttcgat tccgtgttcc agtaaccggc gagcgagttg acgatcgtcg aggcttcggc
      421 gtgttcgcga tatttcggct cgcccgaaag catcagataa gctttcagca aatcgtaatt
      481 tttgccgaga gcttcttctt cagcctgcgt cagcgccgcc ggattcgcca cgttctggct
      541 ttccgtaaat ttccgcaaat cttcttccag acgtttcacg accggcgctt taaatcgctg
      601 ctcgacggcg ttaaagtaaa ttttcagaag gcgttcgcga taaattccat cgcccgaata
      661 aagacccatc cgcatcagcc acggcgcgcc gctgcgctcg tattcgtcga agtcaaccag
      721 attttttcgc aaattttcaa tcgtgttcag ctcgactaaa gcctcgtccg gcgttttcgt
      781 aaacgggtcg cgtccggcgt ccgacttgta aagcgtcaga actttttcgc cgtttgccga
      841 cgcttcgtcc agcaattttt tattttgata aagcgaatac gcggaaagtc cgagcaataa
      901 ggcgaccaga aacgctcccg taatcgttgc gagccagcct aaaatcggcg gtttctgacg
      961 ctgcgcctgc aaggtcgcca ccaaatcctt atcgcgcaga acgacttcgc ggaaaagctt
     1021 ttcggtaaaa tacgtctgcc cgatggtttg cggaatgccg gcgagcgtct gcccgccctg
     1081 cggcattttc gggcgcgtga ccggcacggc ggtaaaataa aaaccgcgca gaaacgggct
     1141 ttcgctaaac gggttcgggc ggaaaagcgt cgaaacaaag gcgccgagct tgcgccgcgc
     1201 cgagccgaaa tgaagcggga aattaaaaat tctcaattgg cgaacgggcg gaaacggcgc
     1261 gcttaagcga atcaggcggc gtttcattat cgagttttgc agaagctcgt attcggcgtc
     1321 gaacagcgaa tgcgcgttgt cggcgttttc cagcggaatc gtcgcgcccc agaccaggtt
     1381 ttgcccttcc ttctgcgaat tggaaaaaga atcgcgaaag ccttcaatcg cgtcggcgtg
     1441 cgtgaaaacc agataaatcg gaaagcggac tttcgtgcgc tgcaccgctt cgtcaatccg
     1501 cgcgcgcaaa accttcgcct gttcttcgat ttccttttcg tcggcgtgca gaacgcgctc
     1561 ggcgctgacg ctcagaatca ttccgtcgag cgggcgcgcg ccgcgatatt ttttgattgt
     1621 ttccagcagc gaagaccatt cttcgccgtc caagccttcg gtttgatagc gtcccgccgt
     1681 atcgacgaaa accgcttcgc tcgtcacgcg ccaatcgacg ccgcgcgtcg ggcggataaa
     1741 tttctgctcg gattgacgct ggctcggcag attttgaaag tttaagttcg accccagaac
     1801 gagcgacgat tttccggctt tcggcgttcc catgaccaga taccacggca gcgaataaac
     1861 gtccagattc gagcttttca gaaactgcgc gacttcttcg gcgcttttcg tcaaatcgcc
     1921 gtttgtgtcc ggcgcggcaa gcttctgcgg tttcacggca tcggttttct gctcggcttt
     1981 tgcgcccgcc gcttcgtccg ttttagcggc ttctttttta gctttgcggc gcgaagcgag
     2041 gtagccgatt atcaacgcaa ccggcaaagt caaaagaatc aaggcgataa tgacaatccg
     2101 cgaattgacc ggataattca ttttcgtccc ggcaacccag acgaccatcg cgacgacccc
     2161 gtaaaaagaa aagagtccgc caagccctaa ggcaaattta agttggtttc cgtaccaggc
     2221 agtcataaaa aagtagtcgg aagttagaag tcagaagtca gtagaaattt tttattctgt
     2281 ctactgactt ctgtcttccg tcttctacaa ttgaagtttc tgcattgcgt cgtccagaaa
     2341 tttcgacgac agtaaaaaca tcaccagata aagcaaaagc ccgaaagcca gaccgccgaa
     2401 agcggcgact tttgcccagg tcggcattcc gcgtttttcg ggcggcttcg gctggtcgtt
     2461 cgcctgccag tgcggcgaaa gctcgacttt cgtgattttt ccggctttca ccagcgcatc
     2521 cgccgtctgc tgcataatgc tgtgcagctt ttcgtgctcg tagacggcgt aacgaccttt
     2581 aaagcccaga agcatacaga cgtaataaat ctcgaccgcg tccgccgtct gctcaatctg
     2641 cgcaatcatc gcctgcagct tttcaaaaaa tttgtttccc gccagctgct cgccgaaata
     2701 ttcgagctgc aaaggatatt tttcccattc ttcctttaaa tgaaaatcgc cggtcagaac
     2761 cgtctcgtca acaaaagaag cgagcgcgaa tttcgccacg ttcacgactt tgtgattaaa
     2821 gcggtaacgc tcggcgcgct gctcgaaatc gtccagcagc gatttgattt tcgggcgcag
     2881 ctcgttcgaa ggcttgacga ttcccgcctg caatcgcaga atcaaatcga aaacgggcgc
     2941 ggcaaacgtc accaaatcgt tggcgttttg ttgctgttgc tgcgagcggc ggctggattg
     3001 tgctctttgt tctgacatcg ttccttaatg taatgcgtaa aatagcagaa aaaaatgtca
     3061 cgtcgaacga aaatttttac ggtttgacgg catacatttc cagtttttcg tccggaattt
     3121 tttccggcac gtaaacggcg atgattttcg agcctttgat gccttcccaa tacgcgccgc
     3181 tcgattccaa ctggaaatag cgaaaaccga cgcgcgtcgg aatcggcgcg ggcggcggat
     3241 tcgagtgcgt caggttgacg cccggcagag cgcgaccgac gaccgtgtcg ataacgtcgc
     3301 gcgaggcgat tttgataacg cgcggaacgt cttcgatgat tttcgcttcc ggcatctgcg
     3361 cgcgaaccgc cagaaaaaat ccggcttcct tcagcagacg ctcgtcttcg acgcgaccga
     3421 cataaagcgt cggattgatt ttttcgagcg gaatcgggac gcagcggctc ggaatcacgg
     3481 tttcgagcaa atctctcaac tggactgaaa gattcagaaa cgtgaaatgc aaatcgtcgt
     3541 ggtcgtattt gacgatgtct ttcggatgta aatcggtcga aaaagtcatc agctgaccga
     3601 cgatttgcga catttccaga taaagctttt cgggatgcag cagcggcgag cgaaaataat
     3661 gcgccatcgt cggaatcgaa gaattgatcg tgtgcagcag ccagaaaacc gcgacttccg
     3721 acgttgtgaa atccgctaaa gaagcgtttc tctgccgccg ctgttcgccg aggct
//
LOCUS       DASWNE010000900         3160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_562098, whole genome shotgun sequence.
ACCESSION   DASWNE010000900 DASWNE010000000
VERSION     DASWNE010000900.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562098"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="VGB68_17625"
     CDS             <1..1038
                     /locus_tag="VGB68_17625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HEY0051118.1"
                     /translation="APVKLKIVGEAVAGRGFDGKIKKGEAIRIMTGAPVPKGADAVQK
                     VELTKENGGFIEILEATKEKQNIIRRAEEIEKGAKVFSAGEIITENMIAALASFGYAK
                     VKAGAKPKVSILATGSEIVDVSEKPAQDQIRNSNSVMLDAFARKSGAETEVLPLVKDD
                     FQILKNQIAAAVGLNDQSRISNFKSQILLITGGVSVGDYDFTKPALRELGAEIFFEKV
                     SLKPGKPTVFAKLNDKLIFGLPGNPVSVAVTFYLFVRAALLKMQGARDCELKKGFAVT
                     GEKIKGAKERDSFLPVSLATDDAGKLIIESLRFRGSSNFIAFSRADALVFVPQGEILE
                     AGAVARIAYLP"
     gene            1182..1649
                     /gene="moaC"
                     /locus_tag="VGB68_17630"
     CDS             1182..1649
                     /gene="moaC"
                     /locus_tag="VGB68_17630"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEY0051119.1"
                     /translation="MSKLSHYDETGEVKMVDVSGKAVTKRRAVASARVLMNAATVRAV
                     REQTNPKGNPLEIARIAGIMAAKQTANLIPLCHQLPLSKVEVKAELKDDGVYLEAEAV
                     TNAQTGVEMEALTAVSVAALTVYDMCKAVQKDMQITEIRLESKEGGKSGTITN"
     gene            1848..2636
                     /locus_tag="VGB68_17635"
     CDS             1848..2636
                     /locus_tag="VGB68_17635"
                     /inference="COORDINATES: protein motif:HMM:NF024790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEY0051120.1"
                     /translation="MKYRLAEDAKPQLWLLLVATIITIALWFIPFADYLVYPIRLFVT
                     FIHESSHALVAFLTGGAVQSLTIASDGSGVVYSAPSGFFGAILTSSAGYLGTTAFGVL
                     LLFLIRRSISPNKVLMACGALVAFVTAFFGILMPIFNIFSLQVGFSSLLFTTFCGAIL
                     SAGLFALGKYASLRTANFAVAFLAVQCLLNAFLDLKTLFFINAPLIGSDLQNDATNMA
                     NATGVPGFVWVLIWIGISVVMISVGLRVYAVSRKGSQTDLPFED"
     gene            2681..>3160
                     /locus_tag="VGB68_17640"
     CDS             2681..>3160
                     /locus_tag="VGB68_17640"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEY0051121.1"
                     /translation="MKLNLVWLFLFVFCINAWAQETVSVKNLVNDYAEVLDEQTEKFL
                     ESRLKDFRAGTDPPVEIAVVTVKTTNKKPIFDYSLAVARNWRKTSRLKNSEGLLLFIS
                     LEDRKYFTQTSSAIQKDLPDAAVAQIQRDFLVPAFRAENYAKGITDTLNAYIAKLAAA
                     "
BASE COUNT          814 a          730 c          796 g          820 t
ORIGIN      
        1 gcgcccgtca aattgaaaat cgtcggcgaa gcggtggcgg gcagaggctt tgacggcaaa
       61 atcaaaaaag gcgaagcaat cagaataatg accggcgcgc ccgtcccgaa aggcgcggac
      121 gcagttcaga aggtcgagct cacaaaagaa aacggcggtt ttatagagat tttagaagcg
      181 accaaagaaa aacaaaatat tattcgccgc gccgaagaaa tcgaaaaagg cgcgaaggtt
      241 ttttcggcgg gcgagataat cacggaaaat atgattgccg cgctggcgtc tttcggctac
      301 gcgaaagtaa aagccggcgc gaaaccgaaa gtttcgattc tagccaccgg aagcgagatt
      361 gtcgacgtct ccgaaaaacc ggcgcaagac caaatccgca attcaaattc ggtaatgctc
      421 gacgctttcg cccgaaaatc gggcgcggaa accgaagttt tgcctttggt gaaagacgat
      481 ttccagattc tgaaaaatca aattgccgct gccgtcggat taaacgacca atctcgaatc
      541 tcgaatttca aatcgcaaat cctgcttatc acgggcggcg tttcggtcgg cgattacgat
      601 tttacgaaac ctgctttgcg cgaattgggc gcggagattt tttttgagaa agtgagcttg
      661 aagcccggca agccgacggt tttcgcaaaa ttaaacgata aattgatttt cggtctgccg
      721 ggaaatccgg tttccgtggc ggttacgttt tatctattcg tgcgagcggc gctcttgaaa
      781 atgcagggcg cgcgcgattg cgaattaaaa aaaggctttg ccgtgacggg cgaaaaaata
      841 aaaggcgcga aagagcgcga ttcgtttttg cccgtctcgc tcgcaacgga cgacgccgga
      901 aaactcatca tcgaatcgct gcgctttcgc ggctcgtcga attttatcgc tttttcgcgc
      961 gccgacgctt tggttttcgt tccgcagggc gaaattttag aagccggagc ggtcgctcga
     1021 attgcttatt taccttaaag agaaattaac agggataaag gggataaaca gggattttta
     1081 agacaaaagg caaaattatg atttaccttc tgtcttttct tatctccttc atccccttta
     1141 tccctgttaa ttttcctgtt gtgctaatct tattttcggt tatgtctaaa ttaagtcatt
     1201 acgacgaaac aggcgaggtt aaaatggttg acgtttcggg aaaagccgta acgaaacgcc
     1261 gcgccgtcgc ttccgccaga gttttgatga acgcggcaac cgtccgagcc gtcagagagc
     1321 aaacgaatcc gaaaggaaat ccgctggaaa ttgcgcgcat cgccggaatt atggcggcaa
     1381 aacaaaccgc gaatctgatt ccgctgtgtc atcagctgcc tttgtcaaaa gtcgaagtaa
     1441 aagccgagct gaaagatgac ggcgtttatc tcgaagccga agccgtgacg aacgcgcaaa
     1501 ccggcgtgga aatggaagct ctgacggctg tctcggtcgc cgcgctgacc gtttacgata
     1561 tgtgcaaagc cgtgcagaaa gatatgcaaa tcacggaaat ccgcctggaa tcgaaggaag
     1621 gcggaaagag cgggacaatt acgaattaaa aattgcgaat tacgaattaa aaagatttaa
     1681 cctttgagct ttgattttta atctttttta tcgagcaaaa tcaaaggtta agactcgtca
     1741 attcgtaatt cgtaattcgt aattcgtaat tgatgagaaa caattacttt gaactttgca
     1801 tcagaaaaag cgtagaatgt tttgtattga ggaaaggagc tttttaaatg aaatacagat
     1861 tagccgaaga tgccaagccg caattatggc tgctgctggt ggcgacgatt attacgattg
     1921 ctttgtggtt tattccgttt gccgattatc tggtttatcc gattcggctt tttgttacct
     1981 ttattcacga aagcagccac gcgctggtcg catttttgac cggcggcgcg gtgcagagcc
     2041 tgaccatcgc ttcggacgga agcggcgtag tttattcggc gccgtcggga tttttcggcg
     2101 caatcctgac ctccagcgca ggctacctgg gcacaacggc gttcggcgtt ttgcttcttt
     2161 ttttaattcg ccgctcaatt tcgccgaata aagttttgat ggcttgcggc gcgctcgtcg
     2221 cttttgttac cgctttcttc ggaattctga tgccgatttt taatatcttt tcgcttcagg
     2281 tcggtttttc gagtttgctt tttacgactt tctgcggagc gattctttcc gccggacttt
     2341 tcgcgctcgg caaatacgcg agcctgagaa cggcgaattt tgccgtcgct tttcttgccg
     2401 ttcaatgtct gctgaacgcc tttttagatt tgaaaacgct cttttttatc aacgcgccgc
     2461 tcatcgggtc tgatttgcaa aacgacgcga cgaatatggc aaacgcgacc ggcgtgccgg
     2521 gcttcgtctg ggttttaatc tggattggaa tttcggtcgt gatgatttcc gtcggcttgc
     2581 gcgtttacgc cgtcagccgg aaaggcagcc agaccgattt gccgtttgaa gattaagaga
     2641 aatttccgcg tcggcgcggc agataaccga agaaataatt atgaagctaa acctggtttg
     2701 gttgttttta tttgttttct gtattaatgc ctgggcgcag gaaacggttt cggtcaaaaa
     2761 tttagttaac gattacgcgg aagtccttga cgagcaaact gagaaatttt tagaatccag
     2821 attaaaggac ttccgcgccg gcaccgaccc gcccgtcgaa atcgcggtcg tcaccgtcaa
     2881 aaccactaat aaaaaaccga ttttcgatta ttctctggct gtcgcccgca actggagaaa
     2941 aaccagccgg ctcaaaaaca gcgaaggtct gcttcttttt atttctctgg aagaccgaaa
     3001 atatttcacg cagaccagca gcgcgattca aaaagatttg cccgacgcgg cggtcgcgca
     3061 aatccagcgc gattttctcg ttcccgcttt tcgcgcggaa aactacgcga aaggcatcac
     3121 ggacacgctt aacgcttata ttgccaaact cgcggcggca
//
LOCUS       DASWNE010000901         2048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_341289, whole genome shotgun sequence.
ACCESSION   DASWNE010000901 DASWNE010000000
VERSION     DASWNE010000901.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341289"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..1036
                     /locus_tag="VGB68_17645"
     CDS             155..1036
                     /locus_tag="VGB68_17645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051122.1"
                     /translation="MATRKKRLQEVKPATAAAGAATTPKETPRYQDAFQRRTERRIED
                     VSRKVEGKGKTILYALGALAVLAILLGIFVSWNRRSNAAAQAALGKAIETSEAQVTTQ
                     PPLAGSTAKTFKTEKERAEASIAEFKGVADKYGSPVREKAQYFIAVNKLSIDRAAAVA
                     ELENLAKSGGEVGTLSKFALAQAKEGDGKFDEAAALYGELAALKDPVISKDTINFALA
                     SIYEKQGKKEDAANLYYNIAKPAYDAKDADGKAIQPSQTAREARTKLQTLDPTRAAEI
                     KEPAPEMPQMPGGFPIG"
     gene            1318..>2048
                     /locus_tag="VGB68_17650"
     CDS             1318..>2048
                     /locus_tag="VGB68_17650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051123.1"
                     /translation="MGEIAQIKTHEVSAGETSVKADFSGKTTSIRVSPNSYFVGIFLT
                     MFLAAFFIYLHMDFAGVLAIIISWLVIPSLLLTDKIIFDGENLTRTGVVPNLWAWINR
                     EPNKLNVGDVEQVETQAMRALKRGGNVFYRYRTAVTGKGLNFAFASGGEDYRQMVKSL
                     FSRLNEDTLDNRSIELRDYLTEPKETLMKAEFARLPSAEVLENSVSEFQISNRNGKRK
                     DSETVVEDHEKAEYLRRLANELRLAG"
BASE COUNT          558 a          510 c          530 g          450 t
ORIGIN      
        1 tcgaaatttt taaaatttgt ttagttcgcc ttgcctttga tgcataaaac agctttgctc
       61 acttgtcact cgccggcatt ttacgctaca tttgagtttt tcgtacaaac tcaagttatc
      121 atccgaacgt ttaagaaagc agaatattgc agtaatggct acaagaaaga aaagattaca
      181 ggaagtaaaa ccggcaaccg cggcggcagg cgcggcgacg acgccgaaag agacgccgcg
      241 ttaccaggac gcttttcagc gccggacgga aagacgcatc gaagacgtca gccgaaaggt
      301 cgaaggcaaa ggcaaaacga ttctttacgc gctgggcgcg ctcgccgttc tggcgattct
      361 actcggaatt ttcgtttcgt ggaatcgtcg ctcgaacgcc gccgcgcaag ccgcgctcgg
      421 caaagctatc gagacctccg aagcgcaggt gacgacgcag ccgcccttag ccggttcgac
      481 cgcgaaaacc tttaaaaccg aaaaggaacg agctgaagcc tcgattgccg aatttaaagg
      541 agtcgccgat aaatacggca gcccggtacg cgaaaaggcg cagtatttta tcgccgtcaa
      601 caagctttcg attgaccgcg ccgccgccgt cgccgaactg gaaaatctgg cgaaatccgg
      661 cggcgaagtc ggcacgcttt cgaaattcgc tctggcgcag gcaaaagaag gcgacggaaa
      721 atttgacgaa gccgccgcgc tctacggcga gctggcggcg ctgaaagacc cggttatctc
      781 caaagacaca atcaatttcg cgctcgcatc gatttacgaa aagcagggca aaaaagaaga
      841 tgccgccaat ctttattaca acatcgccaa gccagcctac gatgccaagg acgcggacgg
      901 aaaagcgatt cagccctcgc agacggcgcg cgaggcgaga accaagcttc agacattaga
      961 cccgacccgc gcggcggaaa tcaaggaacc ggcgccggaa atgccgcaga tgccgggcgg
     1021 ttttccgatt ggctagcttg tcgaaacagg aatttgaaga aacagcccgt cgcgtaagcg
     1081 aaaggggaga aagaaaaaga aatccttaaa ttctttcttt cattttcgtt tggctcgcgg
     1141 gctgatttct ttaagattta aagcatttaa ttcaatttag attcaaaaat ccgatatccg
     1201 aagccgtttg tttattcggt ttcggatatc ggatttttta tttaaggacg gaaaaatttg
     1261 aaaaatcccg tggaattagg tatttataac aatatagact ttttaaagga agaaaacgtg
     1321 ggagaaattg cccagataaa aacgcacgag gtttcggctg gcgaaacttc cgtcaaagcc
     1381 gacttttccg gcaaaaccac cagcattcgc gtatcgccga acagttattt cgtcggcatt
     1441 tttctgacga tgtttctggc ggcgttcttt atctatctgc atatggattt cgccggagtc
     1501 ctggcgatta tcatcagctg gctggtcatc ccgtcgctgc ttttgacgga taaaatcatt
     1561 ttcgacggcg aaaacctgac gcgcaccgga gtggttccga acctttgggc gtggattaat
     1621 cgagagccga ataaattgaa tgtcggcgat gtcgagcagg tcgaaactca ggcgatgcgc
     1681 gctttgaaac gcggcggaaa tgttttttat cgctaccgga ccgccgtcac cggcaaaggt
     1741 ctgaatttcg ctttcgcttc gggcggcgaa gattatcgac agatggtcaa atcgctgttt
     1801 tcccgcctta atgaagacac gctcgacaat cgctcaatcg agctgcgcga ttatctgacc
     1861 gagccgaaag aaaccttgat gaaagccgaa tttgcacggc tgccttcagc cgaagtgctg
     1921 gaaaactcgg tcagcgaatt tcaaatttcc aatcgaaacg gcaaaagaaa agattcggaa
     1981 acggttgtcg aagaccacga gaaagccgaa tatctgcgcc ggctcgccaa cgagctgcgg
     2041 ctcgccgg
//
LOCUS       DASWNE010000902         4326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_666209, whole genome shotgun sequence.
ACCESSION   DASWNE010000902 DASWNE010000000
VERSION     DASWNE010000902.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666209"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..1510
                     /locus_tag="VGB68_17655"
     CDS             254..1510
                     /locus_tag="VGB68_17655"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEY0051124.1"
                     /translation="MNVLVLNCGSSSLKFQLIATDLERIAQDADRRLAHGQIERIGGA
                     AIITFAAEGREPQRSAAPVRDVRAALEAVLRWITSEASGVEGVGGLADIDAVGHRVVH
                     GGEKFRQSVLISDEILRGIEDCIELAPLHNPANIKGILAAREIFGRNIPQAAVFDTAF
                     HQTLPEQSYLYALPYQLYRRHRIRRYGFHGTSHRYVAYRYRRLRNLPRDQVNIITLHL
                     GNGCSIAAIKNGNSMDTSMGLTPLEGLVMGTRAGDLDPAILEFIGDKEGLTIHEVEML
                     LNKQSGLLGISGLTNDMRELLAEADESGDRRARLAIEIFCYRARKYIGAYLAATNGAD
                     AVIFTGGIGENSAQIRSLICAQLTWLGVELDGERNQSHTSGREGVISRDDSRLSVYVV
                     PTNEELLIARDAVRLIHSSGTNPSQG"
     gene            complement(1613..2536)
                     /locus_tag="VGB68_17660"
     CDS             complement(1613..2536)
                     /locus_tag="VGB68_17660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051125.1"
                     /translation="MNKTVLLIFISFIWAFSACGAADFAAGKNSRTLSLNTGKASAES
                     LESTAEKSVDEETENQSNNVTGVYQSKDANHFNEFKIEARGGDLKVEFMGLYEFKTEG
                     GMAMANTDQTDVLTATLKGDTAIVKIPDYPKCRYTLLFAGEKLTVRRDGTIMDCNLGG
                     QIAPEGVYRKVSSKTPVFGEWFEEKKDQAKETPPENQNEAVQPATAKRIRFQPGSSEA
                     VVSGKITNAGAVAYLVNARKGQTLEFRVLEGGKYNDVVAEVFAPDGANLTGEDYGTFW
                     RGELPQSGDYKVSVGTIETENTNFKIQIAIK"
     gene            2956..4311
                     /locus_tag="VGB68_17665"
     CDS             2956..4311
                     /locus_tag="VGB68_17665"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEY0051126.1"
                     /translation="MRFLSGFESTHIFGSGTDVLELTGHTEFVEEDLKLAHSCGLDLL
                     RYSAPWHSIERAPGVYDWRWMDKALDCMRDLGIAPILDPLHHTSFPEWLEGGFANPDF
                     SRVYLKFCTALAERYPWVRHYTVINEPFVTTWFCGHEGVWYPYASGANNFVPMILNVV
                     EAISSVSRMLVERMPDVCLIHVDAAEKHRAVDEVSEPHTAFHNEIRFLVPDLVLGRVD
                     KRHALYNYLRRHGASEKRLARLRAKPARIDVLGLDYYSHCELEWCSRGRVYPNQAPEG
                     LVPTAMEYAERYKLPLMLTETNIRGFVHDRISWLKFMVEQCEEIERRLLPLGLSFEGF
                     CWYPFIDSTDWCSLVKEANGNIDPQGIFYLDKDCRKRNASELSEIFTALARGRISSKD
                     IPAYRFQAPLDHQLEAFLPMMAHWQWREPVTHDAKHVARLQLNRLERKAKRVLVKEAA
                     A"
BASE COUNT          989 a         1120 c         1174 g         1043 t
ORIGIN      
        1 aagattaacg aaaaaaataa aatttttctg tctgtttgct tacataggag tctatcacca
       61 atcacggaat ttttaaatga attttggcga agcaataaaa aaatcaatca ggcagcgaaa
      121 aagacatcgc gccaacagga atgctcggcg caggtctggc atacatttat tttcgcgcgt
      181 tttgccgacc gtaaatttta caagtctttc ggattccgta taaactcatt caacaatcgg
      241 agacaaattt cggatgaatg ttctggtgtt aaattgcggc agctcgtcgc tcaagtttca
      301 gttgattgcg accgacctcg aacgaatcgc gcaggatgcc gaccggcggc tggcgcacgg
      361 tcaaatcgag cgcatcggcg gcgcggcgat tattactttt gccgccgaag gacgcgagcc
      421 gcagcgctcc gccgctccgg tcagagacgt ccgcgcggcg cttgaagcgg ttttgcgctg
      481 gattacgtcg gaagcttcgg gcgtcgaagg cgtcggcggt ctggctgaca tcgacgccgt
      541 cgggcaccgg gtcgtccacg gcggcgaaaa attccgccaa tcggtgctta tctcggacga
      601 aatcctgcgc ggcatcgaag actgcatcga gctggcgccg ctgcataatc cggcaaatat
      661 caaaggcatt ctggcggcgc gcgaaatatt cggcagaaac attccgcagg cggcggtttt
      721 cgatacggcg tttcatcaaa ccctgcccga acaatcgtat ctctatgcgc tgccttatca
      781 gctttatcgc cgtcatcgca tccgccgcta cggttttcac ggcacttcgc atagatatgt
      841 cgcgtatcgc tatcgccgcc tgcgaaactt gccgcgcgac caggtcaata tcatcacgct
      901 gcatctcgga aacggctgct cgattgccgc catcaaaaac gggaactcga tggatacttc
      961 gatgggcttg acgccgctcg aaggcttggt gatggggaca cgcgccggcg acctcgaccc
     1021 ggcaattctg gaatttatcg gcgataagga aggcttgacg attcacgaag tcgagatgct
     1081 tttaaataag cagtcaggtc ttctcggcat ttccggctta accaacgata tgcgcgagct
     1141 gctagccgaa gccgacgaat caggcgaccg ccgcgcgcgg ctcgccatcg agattttctg
     1201 ctatcgcgcc cgcaaataca tcggcgctta cctggcggcg acgaacggcg cggacgcagt
     1261 tatttttacg ggcggaatcg gcgaaaattc cgcgcaaatc cgctcgctga tttgcgcgca
     1321 gttaacctgg ctcggcgtcg agctcgacgg cgagcgaaac caatcgcaca catcgggacg
     1381 cgaaggcgta atcagccgcg acgattcgcg tctgagcgtc tatgtcgtcc cgaccaacga
     1441 agaattattg attgcccgcg acgcggttcg ccttattcat tcttccggca caaacccgtc
     1501 gcaaggctag tcctggaatg caaaacgcga aatgctgaaa cgttcgccgg aaaattttat
     1561 ttccagactg cgctcgtaac atttaatgat ttcatcggcg gatgaaactt ttttatttaa
     1621 tcgcaatctg aattttaaaa ttcgtattct cggtttcgat cgtgccgacg gaaactttat
     1681 aatcgccgct ctgcggcagc tcgccgcgcc agaacgtgcc gtaatcttcg cccgtcaggt
     1741 tagcgccgtc cggcgcgaaa acttccgcga cgacatcgtt atatttgccg ccttcgagaa
     1801 ctctgaattc cagcgtctga ccttttcgcg cgttgaccag ataagcgacg gctccggcgt
     1861 tcgtgatttt gccggaaacg accgcttcgc ttgagcccgg ctgaaagcgt atgcgcttcg
     1921 ccgtcgcggg ctgcacggct tcgttttgat tttcgggcgg cgtttcttta gcttgatctt
     1981 tcttctcttc aaaccactcg ccgaaaacgg gcgttttgct gctgactttg cggtaaacgc
     2041 cttcgggcgc gatttgtccg ccgagattgc aatccataat cgtcccgtcg cgccggacgg
     2101 ttaatttttc gcccgcaaaa aggagcgtgt agcggcattt cggataatcg gggattttga
     2161 caatcgccgt gtcgcctttg agcgtcgccg tcagaacgtc cgtctggtcg gtgtttgcca
     2221 tcgccattcc gccttcggtt ttaaactcgt aaagccccat aaattcgact ttcaggtcgc
     2281 cgccgcgggc ttcgattttg aattcgttaa agtgattcgc gtctttcgac tggtaaacgc
     2341 cggtgacgtt gttcgattga ttttccgttt cctcgtcgac ggatttttct gcggtcgatt
     2401 ccagactttc cgccgaagcc ttcccggtat taagagaaag cgtccggcta tttttgccgg
     2461 cggcgaaatc tgccgcgccg caagccgaaa aagcccagat aaagctgata aaaattaaaa
     2521 gtacggtttt attcattggt attttttcaa aaaaaacaga ccggaaattt gcctgcaaaa
     2581 gtaaggcgta aaaaatacct ttttggtatc acccgaatcg tgaagtcaaa acgattttcg
     2641 ggagccttgt cggtttaaaa acggaagtgt ggtaaaatca atagtttgcg gaaaaagacg
     2701 gcgagagaga tttttccttc gccaggcttc ggcgagcggc gggcgtccgc cggatgaata
     2761 attttcgaaa gcttgaatga agctaatcgt cagcgccgcg gaaaatgaaa attgcgccgc
     2821 ttttgtctac taaccgacag aaaggcgctt ttggaatgtg cttgaattat ttaataagta
     2881 tttgcagcaa ttagagaaat agaaattcaa ggaacatcaa caactttacc agagaaattc
     2941 aagggggaaa cggttttgag atttttgagt ggttttgaaa gcacgcatat tttcggcagc
     3001 ggaaccgatg tgctggaatt gactggacac acggaatttg tcgaagaaga tttgaaatta
     3061 gcgcattcgt gcgggttgga tttgctgcgg tattccgcgc cgtggcacag catcgagcgc
     3121 gcgccgggcg tttacgattg gcgttggatg gacaaagcgc tcgattgtat gcgcgatttg
     3181 ggcatcgcgc cgattctcga cccgcttcat cacacgagct ttcccgaatg gctcgaaggc
     3241 ggtttcgcca atccggattt ttcccgcgta tatcttaaat tctgcacggc tctcgccgag
     3301 cgttacccgt gggtgcgtca ttacacggtt atcaacgaac cgtttgtgac gacgtggttt
     3361 tgcgggcacg aaggcgtctg gtatccgtac gcgagcggcg cgaataattt tgtaccgatg
     3421 attttaaacg tcgtcgaagc aatcagctcg gtcagccgga tgctggtcga aaggatgccg
     3481 gacgtttgtt tgattcacgt cgacgcggcg gagaaacacc gcgccgtcga cgaagtgtcc
     3541 gagccgcaca ccgcgtttca caacgaaatc agatttctgg tgcctgacct tgttttgggc
     3601 agggtcgata agcgacacgc gctttataat tacctgcggc gacacggcgc gagcgaaaaa
     3661 cgactggcgc ggctgcgcgc aaagcccgcg cgaatcgacg ttctggggct cgattattat
     3721 tcgcactgcg agctggaatg gtgcagccgg ggcagagttt atccgaatca agcgccggaa
     3781 ggcttggtgc cgaccgcgat ggagtacgcc gagcgctaca aattgccgct gatgctgacg
     3841 gaaacgaata ttcgaggctt tgttcacgac cgcatttcgt ggctcaaatt tatggtcgag
     3901 caatgcgagg aaatcgagag gcgtctgctg ccgctcggac tttcctttga aggtttctgc
     3961 tggtatccgt ttatcgattc gaccgattgg tgctcgctgg tgaaggaagc caacggcaat
     4021 atcgacccgc agggcatttt ttatctcgat aaagactgcc ggaagcgaaa cgcttcggaa
     4081 ctgagcgaaa ttttcacggc gctggcgcgc gggcggattt cctcgaaaga tattccggct
     4141 tatcgctttc aagcgccgct cgaccaccag ctcgaagcct ttctgccgat gatggcgcac
     4201 tggcaatggc gcgaacccgt tacgcacgac gccaagcacg tagccagact tcagttaaac
     4261 cggctggaaa gaaaagccaa acgtgttctg gtcaaagagg cggcggcttg atgccggcaa
     4321 aatcat
//
LOCUS       DASWNE010000903         4255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_134627, whole genome shotgun sequence.
ACCESSION   DASWNE010000903 DASWNE010000000
VERSION     DASWNE010000903.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134627"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="VGB68_17670"
     CDS             complement(<1..786)
                     /locus_tag="VGB68_17670"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY0051127.1"
                     /translation="MPHPPFIILDERPGQPIYRQIYETIRRSILNGEFHSGRQLPASR
                     LLAKQLGVSRMTVINAYEQLLAEGYLESRAGAGTFVAEHLPEEFLQTPRLERRKGEAE
                     AAPRSLKFSVYGKNISKQIGRILQNNQATPIVPFQHGLAAIDEFPFDIWSKLASKCYQ
                     TLSRNRFGYGEPAGFYPLRESVAAYLKSARAVNCTPEQVIVTNGAQHAFDLIGRILLE
                     AKTEMWVENPCYSGARHSFQSFGAKIVPVPVDKNGFDLAAALRQ"
     gene            918..1529
                     /locus_tag="VGB68_17675"
     CDS             918..1529
                     /locus_tag="VGB68_17675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051128.1"
                     /translation="MNIFKHWRIYLLLGGAIFVVQTHRVFAQPNTDASKTNSRQAAVE
                     RDGQNDFDFEIGTWKTHLKRLLNPLSGSNTWTEYEGITVVRKVWGGRANLVELAVDGP
                     AGRFEGLSLRLYNPQSRQWSLNFAGVKSGQLSAPTIGGFRNGRGEFYNQETYNGRAIF
                     VRFVISDITPNSCRFEQAFSDDGGKTWEVNWIAVDTRVKDESK"
     gene            1539..2063
                     /locus_tag="VGB68_17680"
     CDS             1539..2063
                     /locus_tag="VGB68_17680"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY0051129.1"
                     /translation="MKKSDINPMPVYFERYINAVEAVEFAEAFAESIRQLDRLDKSLL
                     TALGDKRCDAGKWTIREIFQHLIDWERILSYRALLFARCKGSASQNLDENRLASNMNA
                     GRRSIDGLIDELKTVRASTGAMFESFDEKTSLNTGICWKYEISVLATGFTIIGHQAHH
                     LKIIEEKYCQPPNL"
     gene            2292..3140
                     /locus_tag="VGB68_17685"
     CDS             2292..3140
                     /locus_tag="VGB68_17685"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY0051130.1"
                     /translation="MQKILRLYALCFAALALAVFAHGQARPSSDDKTTPLVIGETFTI
                     NSRILNETRRINVYMPPGYAESAGARLPVLYMPDGGIAEDFLHVAGLVQVSVGNGTMR
                     PFLLVGIENTERRRDMTGPTENENDKKIAPRVGGSQPFRKFIRDELMPQIKRRYRTTD
                     ETAIVGESLAGLFVIETFLVEPDLFDTYIAFDPSLWWNNQKLVSGAGARLRARAKPEK
                     TLYFASSGEDTAEITQRFAEILGRSAASGVRWHYEKMPDEKHSTIYHPAALKAFRALF
                     KPATGK"
     gene            3181..3843
                     /locus_tag="VGB68_17690"
     CDS             3181..3843
                     /locus_tag="VGB68_17690"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY0051131.1"
                     /translation="MSETLNPTKRTKLKRIPKLGNFERETIYRILDEAFICHIGFAVG
                     EQPFVIPTSFGRVGDNLVIHGSAASRMMRALSGEIEICFNVTLIDGLVLARSAFHHSM
                     NYRSVVIFGKAKIVTDETEKLCALKAFTEHIVPNRWREVRPPTANELKATTVLSLSLQ
                     EASAKIRTGDPKDDPADYQSDIWAGVIPLRLTAGKPLNDAQLKNGIEVPPSVSGYKYF
                     KK"
BASE COUNT         1082 a         1044 c         1119 g         1010 t
ORIGIN      
        1 ctgcctgagc gccgccgcca aatcaaaacc gtttttatcg accggaacgg gaacaatttt
       61 cgctccgaaa gattgaaacg aatgccgggc gccggaataa cacgggtttt ccacccacat
      121 ctccgttttc gcctccagca aaattctgcc tattaaatcg aaagcgtgct gcgctccgtt
      181 cgtaacgatg acttgttccg gcgtgcagtt gacggctctg gcggatttca gatacgccgc
      241 cacgctctcg cgcaagggat aaaatcccgc cggttcgccg tagccgaagc ggttgcgaga
      301 gagagtttga taacatttac tcgccagttt cgaccagata tcgaagggaa actcgtcaat
      361 cgccgccagc ccgtgctgaa aaggaacaat cggcgtggct tggttatttt gtaaaatcct
      421 gccgatttgc tttgaaatat ttttcccgta gacggaaaat tttaagctgc gcggcgcagc
      481 ttccgcctcg cctttccgcc tttctaaccg gggcgtttgc aaaaattcct ccggcagatg
      541 ttcggcgaca aaagttcccg cgcccgcccg gctttccaga taaccttccg ccagcagctg
      601 ctcgtaagcg tttatgacgg tcatccgcga tacgccgagt tgttttgcca gcaggcgcga
      661 agcgggcagc tgcctgccgg aatgaaattc gccgttcaaa atcgagcggc gaatcgtttc
      721 gtaaatctgc cggtaaatgg gctgtccggg gcgttcgtcg agtatgataa aaggcgggtg
      781 cggcatattc aaaaattata aattggtctg cctcgtttgt ccaaaactgg ctattgtgac
      841 atcaccaatt ttcattataa tctcctacca gacgataaaa aatcaataaa agaatttaaa
      901 gaaaaaagcg tatggcgatg aacatattta aacattggcg aatttatctg ctgttaggcg
      961 gcgcgatttt tgtcgtgcaa acacatcgag ttttcgcgca gccgaacacg gacgcatcaa
     1021 aaacaaactc ccggcaagcc gccgtagagc gagacgggca aaacgatttt gactttgaaa
     1081 tcggcacctg gaaaactcac ttgaagcgtc ttctgaatcc cttatccggc tcgaatacct
     1141 ggacggaata tgaaggaatt accgtcgtgc gcaaggtttg gggcgggcgc gccaatctgg
     1201 tcgagctggc ggtcgacgga ccggcgggtc gtttcgaagg actgagcctg cggctctaca
     1261 atccccaatc gcgccagtgg agcctgaatt ttgccggcgt caagagcggt cagctgagcg
     1321 cgccgacaat cggcggcttc agaaacgggc gaggcgagtt ttacaatcag gagacatata
     1381 acgggcgagc tatttttgtc cgatttgtca tctcggatat tacgccgaat tcctgtcgtt
     1441 ttgaacaagc cttttctgat gacggcggca aaacctggga agtaaactgg attgcggtgg
     1501 acacgcgcgt aaaagatgaa tcaaaataaa attatccgat gaaaaaatca gacatcaacc
     1561 cgatgcccgt gtatttcgag cgctacatta acgcggtcga ggcggtcgag ttcgcggagg
     1621 cttttgccga aagcatccgg cagctcgacc gattggacaa aagcctgctg acagcgctcg
     1681 gagacaaacg ctgcgatgcc gggaaatgga caatccggga aatttttcag cacctcatcg
     1741 actgggagcg cattttgtct tacagggctt tgctttttgc cagatgcaaa ggctcggctt
     1801 cgcaaaatct cgacgaaaat cggctcgcgt cgaatatgaa cgccggacgc cgttcgatag
     1861 acggattgat tgacgaattg aagacggttc gcgcttcgac cggagcgatg tttgaaagct
     1921 tcgatgaaaa gacctcgctc aatacgggaa tttgctggaa gtatgaaatt tcggttttgg
     1981 cgacgggttt tacaatcatc ggacatcagg ctcatcacct gaaaatcatc gaagaaaaat
     2041 actgtcagcc gcctaatctg taatcgagcg gcttttattt tgtccttaac atttgtgaac
     2101 agttgaaaat tcaggctttg cacaaaaata aagaaaggga aaattgtaaa ttctaaattg
     2161 taaattctaa attgatgttc ggtatctttt gtgaagtaat ttagaattaa gtaaattaat
     2221 aattgagaat ttacaattag ccatcggaat tgttctcgat tttctttatt tttgtgcaaa
     2281 gcagaaaatt tatgcaaaaa attttgagat tatacgcctt gtgcttcgct gctctggcgc
     2341 tggcagtctt cgcgcacgga caagctcgcc cgtcttcgga tgataagacg acgccgctgg
     2401 tcatcggcga gacgtttacc atcaattcca gaattctgaa cgaaacgcgg cgcatcaacg
     2461 tttatatgcc gcccggctat gccgaatccg ccggcgcgcg gctgcccgtg ctttatatgc
     2521 ctgacggcgg catagccgaa gactttctgc acgtagccgg tctggttcag gtatcggtcg
     2581 gcaatgggac gatgcgacca ttcctgctgg taggcatcga aaataccgag cgccgacgcg
     2641 atatgaccgg acctaccgaa aatgagaacg ataaaaagat tgcgccgcgt gtcggaggct
     2701 cccagccttt tagaaaattt atccgcgacg agctgatgcc gcagataaaa cggcgctacc
     2761 gcacgaccga cgaaacggca atcgtgggcg agtcgcttgc cggtttgttc gtgatcgaga
     2821 cttttctggt cgagccggat ttgtttgata cttatatcgc cttcgacccg agcttgtggt
     2881 ggaacaatca aaaactggtg agcggcgcgg gcgcgcgttt gcgcgcgcgg gcgaagccgg
     2941 aaaagacttt gtattttgcg agcagcggcg aagacacggc ggaaatcacg cagcgttttg
     3001 cggaaattct gggcaggagc gcggcgtccg gcgttcgctg gcattatgag aaaatgccgg
     3061 atgaaaaaca ttcgacgatt tatcatccgg cagcgctgaa agcctttcgc gcgctgttca
     3121 aaccggcgac aggcaagtaa ccgcagatgt cgtcggcgac gagcaaagcg tctgaaaaat
     3181 atgagtgaaa ctttgaatcc gaccaaaaga acaaaactga aacgaatacc gaagctcggc
     3241 aatttcgagc gtgaaacgat ttaccggatt ctggatgaag cctttatctg tcatatcggt
     3301 tttgcggtcg gcgagcagcc gttcgtcatt ccgacttctt tcgggcgcgt cggcgataat
     3361 ctggtgattc acggctcggc ggcaagccgg atgatgcgag ctttgagcgg cgagatagag
     3421 atttgcttta acgtcacgct gattgacggg ctggtgctgg cgcggtcggc gtttcatcat
     3481 tcgatgaatt accgctcggt cgtgattttc ggcaaagcga aaatcgtcac ggacgagacg
     3541 gaaaagctct gtgcgctgaa agcctttacc gagcatattg tcccgaaccg ttggcgggaa
     3601 gttcgcccgc cgaccgccaa tgagctaaaa gcgaccaccg tgctttcgct ttctttgcag
     3661 gaagcgtcgg cgaaaattcg gacgggcgac ccgaaagacg acccggcgga ttaccagtcc
     3721 gacatctggg cgggcgtcat accgctgcgt ctaacggctg gcaaaccgct caacgacgcg
     3781 caattaaaaa acggaatcga agttccgccc tcggtttccg gttataaata tttcaaaaaa
     3841 taaaccgggc aatctgtgtt ttttaaacaa atgcgctctt tatttcgcat taaatcgcgc
     3901 gcgagcggcg acgccgaaag tgccgaaaaa actttaggac aaagctcgcg aattttgttt
     3961 ttcgccggca gagcgaaaaa gccgcgggca tttaaaatat aaagaccccg gcatatccgc
     4021 agcgattacc cgatttcgtt tcggcgggcg gttaaatgca aagacgccgc ttccgataat
     4081 tactatcttt ctgcgtaggc gaattgcgat aaattcacgg caaaaactaa atcgtcgttc
     4141 gtgcagggct ggaatatctt tttcgacccg tctcgctttc gctaatgaac agcgcttttt
     4201 tttgcggttt tacgtcgcag agcgctttca gcccgccgcg aaaaaacaaa aactt
//
LOCUS       DASWNE010000904         8785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_65646, whole genome shotgun sequence.
ACCESSION   DASWNE010000904 DASWNE010000000
VERSION     DASWNE010000904.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8785
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65646"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VGB68_17695"
     CDS             complement(<1..513)
                     /locus_tag="VGB68_17695"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0051132.1"
                     /translation="MPAVAVENLSKTYPASFPRLKKLLRISIKPPVEALKNVAFEIET
                     GEIFGLIGRNGAGKTTLTKIIATLVQPTAGKVSVKGFDSIRDEVKVRALVGLATAEER
                     SFYWRLSCEQNMMFFARLYGMNDREARRKIGELFEKLNLNELAKRRFSTISTGNKQRL
                     AVARALLPEPP"
     gene            complement(516..803)
                     /locus_tag="VGB68_17700"
     CDS             complement(516..803)
                     /locus_tag="VGB68_17700"
                     /inference="COORDINATES: protein motif:HMM:NF025742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEY0051133.1"
                     /translation="MNTTDKTEKELTFEVAEADYEEGLEKGWTSDDMLKPGVYKVRRP
                     ARFRQKPGEKVTISLDGDIVDFFRQRASESYEEEINAELRKLIEREKLKAA"
     gene            complement(1043..1858)
                     /locus_tag="VGB68_17705"
     CDS             complement(1043..1858)
                     /locus_tag="VGB68_17705"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0051134.1"
                     /translation="MNRLTYIGIFIAVVLIIAAIFAPLIATQDPVAQNLAMRHAAPSA
                     EHWFGTDAIGRDIFSRLVYGARVSLEVGIIVTLVSTLIGMFIGGIAGFYGGIVDKILS
                     GYIFNVFLAFPGLLLAIALVAFLGAGLEKVILALCIIGWVGYARVMRGQVLKVREYDF
                     VQAARALGASDRRILFTHILPNAIQPLIVQASLGMAGAVLSEASLSFLGLGIPPPAPS
                     WGTMIDEARGIDTMYEAPHVLLFPGIAIALTVLAFNFIGDGLREYLDPKQRKR"
     gene            complement(1886..2032)
                     /locus_tag="VGB68_17710"
     CDS             complement(1886..2032)
                     /locus_tag="VGB68_17710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051135.1"
                     /translation="MIWKFNDAQSRRENNISKEQILNKAFKIAGGSNFLWKNIERRIE
                     EFNL"
     gene            complement(2053..3051)
                     /locus_tag="VGB68_17715"
     CDS             complement(2053..3051)
                     /locus_tag="VGB68_17715"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0051136.1"
                     /translation="MKYKILNFARRLALMLLVVWTVVSLVTLLIIIIPGRPSLAILGE
                     NATGPQIEQFERKHGLDKPPFFFSGSIILNNPFASEIKWHGFDNQYVNYFAGLLRGDL
                     GKSFRTDRPVADMILERYPNTIKLALAAMLVAVTIAIPLGVLAGTNKGSLIDNFSSFF
                     ALLGISLPSFVIGPFLVYLFAVKLNWFAPTGSLNPEDIILPAVTLGAALSAILTRMIR
                     SSVIEELGEDYVRTARAKGLSEKIVIYKHVLKNGLIPVVTILGLQLGVLLAGAIITEK
                     IFGWNGLGTLLLDDGIAKLDYRLIQGCVLIISITYILANSLTDMVYRWLDPRIRLG"
     gene            complement(3086..3814)
                     /gene="ubiE"
                     /locus_tag="VGB68_17720"
     CDS             complement(3086..3814)
                     /gene="ubiE"
                     /locus_tag="VGB68_17720"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEY0051137.1"
                     /translation="MPNERTADELKHARAVREMFSGIAGKYDFLNHFLSVNTDKRWRR
                     LVAKKLQDVLDKPGAMVLDVACGTGDLSLEMQKSGKAKIIGTDFCRPMLSVAREKNQK
                     NQTAIPYIEGDGMNLSFADDTFDAVTIAFGLRNFSNWLDGLKELHRVLKPNGKLAILE
                     FSTPIIPGFKQIFNLYFTQVLPRIGGAVSGSRGAYEYLPNSVSRFPDQKNLALMMGEV
                     GFSDVEYKNLTGGIAALHSGAKKL"
     gene            complement(3817..5103)
                     /locus_tag="VGB68_17725"
     CDS             complement(3817..5103)
                     /locus_tag="VGB68_17725"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0051138.1"
                     /translation="MNYFKRTAFLLVLMLFAANFSFAQSAAQPRQEKLLNGLKVLIWN
                     EPKAEKVSLRLRIHSGSAFDPKDKMGTMALLTDILFPTEQTKNFFEEDLEGSLEVTSN
                     YDYIQINATAKSDEFLTILETIATVVSNPAITQENFVKVRDARLKAVEEMQKNPAYIA
                     DRAIAKRLLGDFPYGRPADGTPESLKLIERADLLTAKDKFFTADNATLAITGSVKHDF
                     AYMAARRLFGAWKKADKLVPATFRQPDDPDTKALSAELPAGEKEQIMRFAIRGLARND
                     KDFWASAVLFKILQNRSLDEFKTRNSFIRHEARLLPGLIVFGSPVQFEVPNGSQPPKP
                     LNFIGKRIQNAISAAEFEKAKAEILDDFNKKNATDLWLDVDTYKLASVKDDAQNAKNI
                     APEDVQRAFERLKTAPEVIILFSPRAATGESTPPKN"
     gene            5383..6699
                     /locus_tag="VGB68_17730"
     CDS             5383..6699
                     /locus_tag="VGB68_17730"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY0051139.1"
                     /translation="MGKIVKYCAACEEGFAAKFAFCPNCGGALTAYELNPVADKANVN
                     QAENANPQAAASAPLMAETSFTEKAPVEPETVAFATDHKEEKFLDDEYLPEETAAFTA
                     NANGSDFRADDFSESQYQTNFQPGGYQPTDAGGYHITVIEERNSGIRNVLLLGSCAFV
                     ITMCLAGVIYSLFTKELYAAGLSNTDLMNAMVVDDVPAEVEEQPKKNKDKGGGGGGGG
                     KDEEDPVSKGQLARQTPVQLTPPSARMPRFEAELKTPVSTQGNQERKITDDPYGDPLS
                     QSSKLSDGGGRGGGQGNGQGTGQGNGRGTGQGNGIGSGSGNGDGSGNGDGKGDGDGDG
                     VTPPPLKRPEPKPVGPTEGVKIISKPRANYTDAARTNNIQGVVRVRVTFLANGTIGSI
                     SPVSNLGYGLTEQALAAARSIRFEPAKKGGVPYSVTKVVEYTFTLY"
     gene            complement(6779..7159)
                     /locus_tag="VGB68_17735"
     CDS             complement(6779..7159)
                     /locus_tag="VGB68_17735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051140.1"
                     /translation="MRCYQKFEIGTALAASVGFVVIFFLFMRFAFQSEPADPQEIFHF
                     GCYIFGGWAVSLVLVAGAYYDVTKPMDKAGRIMILTGWAYIALVWGGTAFFIFIWGAP
                     VAALITVSPVILSSFTVLMAYSND"
     gene            complement(7175..8644)
                     /locus_tag="VGB68_17740"
     CDS             complement(7175..8644)
                     /locus_tag="VGB68_17740"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEY0051141.1"
                     /translation="MKSNELRKHFFIFSIFAAVVTFVLVTLPFIEPVLRQYVVVFFGT
                     TDARIGETAGSLFDTLLKVVKIFIWMGLIFAVVRFFNSLITGTILRNTGSSELTTLIR
                     NVISIVIFVVAFTIVVKSQFDTDLTTFFAGSTILAVVIGLALQDTLGNLFAGIAMQAD
                     QPFQVGDVVNIPNKGTGVVENVTWRGVKIRTFQNKIVLISNAVLGKEAIEVAPRDNLN
                     ARLVFFNTLYSASPARTIHLIREVVRQVENVSPKIRPIVRVRNLGDNGIDWEIKYWLE
                     DYSKFNDTDALIRLRVWYAFQREDIHFAFPTRTLYIENQSLEPNFEESSGAIFERLSE
                     VPLFAPLTDEETEKLARASEAKIFSPNEPIVRKGQEGGSMFVIHHGSVKVQIAENGIP
                     KTINTLTEGEFFGEMGLFTGEPRSANVVAAEETEVLEIKQAALKPLFDANPELVEALS
                     KTIAERRALLTQKNTENNQTTEEESKGIFNSIKSFFGLS"
BASE COUNT         2201 a         2279 c         2254 g         2051 t
ORIGIN      
        1 cggcggctcg ggaagcaaag cgcgcgcgac ggcgagccgc tgcttattgc cggtcgaaat
       61 cgtggaaaaa cgacgctttg ccagctcgtt taaattcagt ttttcaaaca gctcgccgat
      121 tttccggcgc gcctcccggt cgttcattcc gtaaagccgc gcgaaaaaca tcatattctg
      181 ctcacaggaa agccgccagt aaaagctgcg ctcttcggcg gtcgccaagc cgaccagcgc
      241 gcgcacttta acttcatctc tgattgaatc gaaacccttc accgaaactt ttccggcggt
      301 cggctgaacc agcgtggcga tgattttcgt gagcgtcgtc ttgcccgctc cgtttctgcc
      361 gataagcccg aaaatttcgc ccgtttctat ttcaaacgca acgtttttca aggcttcgac
      421 cggcggttta atcgaaattc tcagaagctt tttgaggcgc ggaaatgacg ccgggtaggt
      481 tttggaaaga ttttcgacgg cgacggcggg catttttatg cggctttcag tttttcccgt
      541 tcaattagtt tccgcagctc ggcattaatt tcttcttcgt aagattcgct cgctcgttga
      601 cgaaaaaaat cgacaatgtc gccgtccagt gaaatcgtca ctttttctcc aggcttttga
      661 cgaaatcggg cggggcggcg gactttataa actccgggct tgagcatatc gtcactcgtc
      721 cagccctttt ccaagccttc ttcataatcg gcttcagcca cttcaaacgt caattccttt
      781 tcggttttat ccgttgtatt cattataaat tctttcctcg cgcgtctcgg cttttctggc
      841 tgagattagg cgaataagct ctcgtccgtc tttttcctga acgctataaa caacaaataa
      901 tagtttttcg cccgataacc cgacccggca gaatctttct tcttcgtctg aatgaaaatc
      961 atcaaaaaac tcgattgcgt ttaaattgtt gccttattgt cgtcccaaac aaattccata
     1021 gaaccgtaaa gattttacca tattatcttt tacgttgttt cgggtctaaa tattcgcgca
     1081 agccgtcgcc gatgaaatta aaagccagca ccgttaaagc aatcgcaatg ccggggaaaa
     1141 gcaagacgtg cggcgcttca tacatcgtat ctattccgcg cgcttcgtca atcatcgtgc
     1201 cccagctcgg cgcgggcggc ggaattccca gaccgagaaa cgacaaagac gcttcggaca
     1261 aaaccgcgcc cgccattccc aaactcgcct gcacgattaa aggctgaatc gcattcggca
     1321 gaatatgcgt gaacaaaatt cgccggtcgc tggcgcccaa agcccgcgcc gcctgcacaa
     1381 aatcgtattc gcggactttt aaaacctgcc cgcgcatcac gcgcgcatac ccgacccagc
     1441 cgataatgca gagcgcgaga atcacttttt cgagtcccgc gccgagaaaa gcgaccagcg
     1501 caatcgccag cagcaagccg ggaaaggcga gaaagacgtt gaaaatatag cccgacagaa
     1561 ttttatcgac gatgccgccg taaaatccgg cgattcctcc gataaacatc ccgatcagcg
     1621 tggaaacgag cgtgacgatt attccgactt ccagcgaaac ccgcgcgccg tagacgagcc
     1681 gcgaaaaaat gtcgcgcccg atggcgtccg ttccgaacca gtgctcagcc gaaggcgcgg
     1741 cgtggcgcat cgccagattc tgcgcaaccg ggtcttgcgt ggcgataagc ggcgcgaaaa
     1801 tcgccgcgat tatcagcacg acggcgatga agattccgat atatgtaagt ctattcatta
     1861 atgactcgca gcagcagcag cttctttaca gattgaattc ctcaatccgg cgctcgatgt
     1921 ttttccacaa aaagttactg ccgcccgcga ttttaaaagc cttgtttaaa atttgttctt
     1981 tggagatatt gttttcgcgc cggctttgag cgtcattaaa tttccaaatc aatcgaaaaa
     2041 gtgttcacaa atttaaccta atctgattct cgggtcgagc cagcggtaaa ccatatcggt
     2101 caggctgttt gccagaatat acgtgatgct gataatcaga acgcagcctt gaatcaaccg
     2161 gtaatcgagc ttggcgattc cgtcgtcgag cagcaaggtt ccgagcccgt tccagccgaa
     2221 aattttctcg gtaataatcg ctccggcgag aagcacgccg agctgcaagc ccaaaatcgt
     2281 gaccaccgga atcaagccgt ttttcaaaac gtgcttgtag ataacgattt tctcgctcaa
     2341 gcctttggcg cgcgccgtgc ggacgtaatc ttcgccgagt tcttcgatga ccgacgagcg
     2401 aatcattcgc gtcagaatcg ccgaaagcgc cgcgcccaaa gtgacggcgg gcagaatgat
     2461 gtcttccggg ttcaagctgc cggtcggcgc aaaccagttt aatttgacgg cgaacaaata
     2521 aactaaaaac ggtccgatga cgaagctcgg caggctgatg ccgagcagcg cgaaaaagga
     2581 cgaaaaatta tcaatcagag agcctttatt agtgcccgcc agcacgccga gcggaatcgc
     2641 aatcgtcacc gccacgagca tcgccgccag cgccagcttt atcgtgttcg gatagcgctc
     2701 cagaatcata tcggcaaccg ggcggtcggt gcggaaagac tttcccaaat cgccgcgcaa
     2761 aagtccggcg aaataattca cgtattggtt gtcgaaaccg tgccatttga tttccgaagc
     2821 gaaaggatta ttcagaatga tgcttccgct gaagaaaaac gggggtttgt cgagcccgtg
     2881 cttgcgctcg aattgctcga tttgcggtcc ggtcgcgttt tctcccagga tggctaacga
     2941 cgggcgaccc ggaattatga tgatgagcag ggtaacgagc gagacgaccg tccagacgac
     3001 gagcagcatc agcgcgagac ggcgtgcgaa atttaaaatt ttgtatttca tcggaaagaa
     3061 gcgcgcgaaa agccttcgaa aatagttata actttttcgc gcccgaatgc aaagcggcga
     3121 ttccgcccgt cagatttttg tattccacgt cggaaaatcc gacctcgccc atcatcagcg
     3181 ccagattttt ctggtcggga aagcgcgaaa ccgagtttgg gagatattca taggctccgc
     3241 gcgagccgct gaccgcgccg ccgatgcgcg gcagaacctg cgtgaaataa agattgaaaa
     3301 tttgtttgaa gcccggaata atcggcgtgg aaaattcgag aatcgcgagt tttccgttcg
     3361 gcttcaaaac gcggtgaagc tcttttaagc cgtccagcca gtttgaaaaa tttcgcaagc
     3421 cgaaagcgat ggtcacggcg tcgaaagtgt cgtcggcaaa cgaaagattc attccgtcgc
     3481 cttcgatgta gggaatggcg gtctggtttt tttgattttt ttcacgggcg acgctgagca
     3541 tcgggcggca aaaatccgtg ccgatgattt ttgctttgcc ggatttttgc atctccagcg
     3601 acaaatcgcc agtgccgcag gcgacgtcca gaaccatcgc tccgggcttg tccaaaacgt
     3661 cctgcaattt tttggcgacc agcctgcgcc agcgtttatc ggtattgacc gacaggaaat
     3721 gattcagaaa atcgtatttt ccggcaatgc cggaaaacat ctcgcggacg gcgcgggcgt
     3781 gttttaattc gtcggcggtt ctttcgttcg gcatttttaa tttttaggcg gagtcgattc
     3841 tccggtcgcg gctctgggag aaaacaggat gattacttcg ggcgcggttt ttaagcgctc
     3901 aaaggctctc tgaacgtctt ccggagcgat gtttttggcg ttttgcgcgt cgtcttttac
     3961 cgaagccagc ttataagtat cgacatccag ccaaaggtcg gtcgcattct tcttattgaa
     4021 atcatctaaa atttcggctt tcgccttttc gaactcggcg gcggaaatcg cgttttgaat
     4081 ccttttcccg ataaaattaa gcggtttagg cggctgagag ccgttcggaa cctcaaactg
     4141 aaccggcgag ccgaaaacaa tcaagcccgg cagaagccgc gcttcgtggc ggataaagga
     4201 gttccgggtt ttaaattcgt cgagagagcg gttttgcaga attttaaaca gcacggcgga
     4261 cgcccagaaa tccttgtcgt ttctcgccag cccgcgtatt gcaaaacgca ttatttgttc
     4321 cttttcgccc gccggaagct cggctgacaa tgctttcgtg tccgggtcgt ccggctggcg
     4381 aaaggtcgcc ggaactaatt tgtcggcttt tttccaggcg ccgaacaatc ggcgcgccgc
     4441 catatacgca aaatcgtgct tgacgctgcc ggtaatcgcc agcgtcgcgt tgtcggcggt
     4501 aaaaaatttg tctttcgccg tcagcaaatc ggcgcgctcg attaatttca ggctttccgg
     4561 cgttccgtct gccggtctgc cgtacggaaa atcgccgaga agacgtttgg cgattgcgcg
     4621 gtcggcgata taagccggat ttttctgcat ttcctcgacc gctttcagtc gcgcgtcgcg
     4681 gactttgacg aaattttcct gcgtgattgc cgggttggaa acgacggtcg cgatggtttc
     4741 cagaatcgtc agaaactcgt ccgatttcgc cgtcgcgttg atttgaatgt aatcgtaatt
     4801 gctcgtgact tcgaggctgc cttccaaatc ttcttcgaaa aagttttttg tctgctcggt
     4861 cggaaataaa atgtcggtca gcagcgccat cgtgcccatt ttatctttcg ggtcaaacgc
     4921 cgagccgctg tgaatacgca ggcggagcga gactttttcg gctttcggct cgttccagat
     4981 taaaactttc aagccgttca gaagcttttc ctgacgcggc tgcgccgcgc tctgggcgaa
     5041 agaaaaattc gccgcgaaaa gcattaaaac tagcaaaaac gcggttcttt tgaaataatt
     5101 catattagtt gtcttttatc atttcagccg ggacgcaaaa aacatagatg tcgaatgata
     5161 aatcttagat gcccgcaggc atcccgtcaa ataaactgag catccatatg aatgcaagta
     5221 acgcatctat aatattttgt taagtatatt caaaaactta acattcctga aaatcgtatt
     5281 ataataaaca taaccctttg gcggtcgttc cagtcttgga gggcatcgag tgaacataga
     5341 aaaggtggtt tcattctagt atttgagagg tattgagtga agatgggaaa gatagttaag
     5401 tattgtgccg catgtgagga aggctttgcc gcgaagtttg cgttttgtcc aaactgcggc
     5461 ggagcgttga ccgcttacga attaaatccg gtagcggaca aagcaaatgt caatcaagct
     5521 gaaaacgcga atccgcaagc cgcggcttcc gcgccgctga tggcggaaac ttcatttacg
     5581 gaaaaagctc cggtcgagcc ggaaaccgtc gcttttgcga ccgaccataa agaagagaaa
     5641 tttctggacg acgaatatct gcccgaagaa accgcggcat ttacggcgaa cgccaacggc
     5701 agcgattttc gcgccgacga tttcagcgaa agccaatacc agacaaattt tcaacccggc
     5761 ggttatcagc cgaccgacgc cggcggttat cacatcacgg ttatcgaaga aagaaattcg
     5821 ggcatccgca acgtgcttct tctaggctcg tgcgcgtttg tcataactat gtgtctcgcc
     5881 ggcgtcatct actcgctttt taccaaggaa ttatacgccg ccggactgag caacacggat
     5941 ttgatgaatg cgatggtggt ggacgatgtt cctgccgaag tcgaagaaca gccgaaaaag
     6001 aataaggaca aaggcggcgg cggcggcggc ggcggtaaag acgaagaaga ccccgtttcc
     6061 aaaggtcagc ttgccaggca aacgccggta caactgactc cgccgtcggc gagaatgccg
     6121 cgttttgaag ccgagctgaa aacgccggtt tcgacgcagg gcaatcagga aagaaaaata
     6181 accgacgacc catacggcga cccgctttcc caatccagta aattatctga cggcggcggt
     6241 cgcggcggcg gtcagggcaa cggtcaggga accggacaag gcaacgggcg cggaaccgga
     6301 caagggaacg gcatcggctc cggcagcgga aacggcgacg gcagcggaaa cggcgacggc
     6361 aaaggcgatg gtgacggcga cggcgtcacg ccgccgcctc tcaaaagacc tgaaccgaaa
     6421 ccggtcggtc cgaccgaagg cgtaaaaatt atctcgaaac cgcgcgccaa ttacacggac
     6481 gcggcgcgga caaacaatat tcagggcgtc gtccgggtgc gcgtgacgtt tctggctaac
     6541 ggaaccatcg gcagcatctc gccggtcagc aatctgggct acggtttgac cgagcaggcg
     6601 ctggcggcgg cgcgctcgat tcgtttcgaa cccgcgaaaa aaggcggcgt tccttattcg
     6661 gtgacaaagg tcgtggaata cacctttacg ctgtattaaa aaccgcgttt aataaaaaaa
     6721 caaaaaaggc tggcagtctc aaaaagccag cctttttatg tgttttaaag agcctccgtc
     6781 aatcattgga atatgccata agaacggtaa acgatgacag tataaccgga ctaaccgtta
     6841 ttaaagctgc gacgggcgcg ccccagataa aaataaaaaa cgccgtgcct ccccagacaa
     6901 gcgcaatata agcccatccg gttaaaatca taattcttcc cgccttgtcc atcggttttg
     6961 taacatcata ataagccccg gcgacgagca ctaatgatac cgcccagccg ccgaagatgt
     7021 aacagccgaa atgaaagatt tcctgcgggt ctgccggttc ggattgaaaa gcgaagcgca
     7081 taaataaaaa aaagataacc acaaaaccga ctgaagccgc cagcgccgtg cctatctcaa
     7141 atttttgata acatcgcata ttttttcgcg ccgtctaact cagaccgaaa aaactcttaa
     7201 tcgaattaaa aatgcctttg gattcttctt ccgtcgtctg attattttcg gtatttttct
     7261 gcgtcagaag cgcgcggcgt tcggcgattg ttttgcttaa ggcttcgacg agctccgggt
     7321 tggcgtcgaa aagcggcttg agggctgcct gtttgatttc cagaacctcg gtttcttcgg
     7381 cggcgacgac gtttgccgag cgcggctcgc ccgtaaaaag tcccatctcg ccgaaaaatt
     7441 cgccttcggt cagggtgttg atggttttcg gaataccgtt ttccgcaatc tggactttaa
     7501 ccgagccgtg atgaatgacg aacatcgagc cgccttcctg acctttgcga acaatcggct
     7561 cgttgggcga aaagattttc gcttcgctcg cccgggcgag cttttcggtt tcctcgtccg
     7621 tcaaaggcgc gaaaagcgga acttcactta aacgctcgaa aatcgcgccg ctcgattctt
     7681 caaaattcgg ctccagcgat tgattttcga tatagagcgt gcgcgtcggg aaagcgaaat
     7741 gaatatcttc gcgctggaaa gcgtaccaga cgcgcaggcg tataagcgcg tcggtatcgt
     7801 taaatttcga ataatcttcg agccagtatt tgatttccca gtcgatgccg ttatcgccca
     7861 ggtttcggac gcgcacaatc gggcgaatct tcggcgagac gttttcgacc tgcctgacga
     7921 cttcgcgaat caggtgaatc gtccgcgcgg gcgaagccga ataaagcgtg ttgaaaaaaa
     7981 ccaggcgcgc gttcagattg tcgcgcggcg cgacctcgat cgcttccttg ccgagcacgg
     8041 cgttgctgat aagaacgatt ttgttttgaa acgtgcggat tttgacgccg cgccacgtga
     8101 cgttttcgac cacgcccgtg cctttgttcg gaatattgac gacgtcgccg acctgaaacg
     8161 gctggtcagc ctgcatggcg atgccggcga aaagattgcc gagcgtgtcc tgcagagcga
     8221 gaccgatgac gaccgccaga atcgtcgaac cggcaaagaa cgtcgtcaaa tcggtatcaa
     8281 actgcgattt gacgacaatc gtgaaggcga ccacgaaaat gactatggaa atgacgttgc
     8341 ggataagcgt cgtcagctcg gagctgcccg tattgcgcag aatcgttccc gttatcagcg
     8401 aattgaaaaa gcggacgacg gcgaaaatca agcccatcca gataaaaatc ttgacgactt
     8461 tcagcagagt gtcgaaaagg ctgcccgccg tctcgcctat tcgcgcgtcg gtcgtgccga
     8521 aaaaaacgac gacgtattgt ctgagaaccg gctcgataaa cggcagcgtc accagcacga
     8581 acgtgacgac ggcggcaaag atgctgaaaa tgaaaaaatg ttttcgcagt tcgttggatt
     8641 tcatattcgg aagcttcagc cacaaattaa cacgaatcgg ctgaaactgc gaggcagttt
     8701 caattacgaa ttacaaatta cgaattacga attggaagaa atgactttcg agcctgaaac
     8761 ttcgatatta ctcaatcttc aaaaa
//
LOCUS       DASWNE010000905         2390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_595536, whole genome shotgun sequence.
ACCESSION   DASWNE010000905 DASWNE010000000
VERSION     DASWNE010000905.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595536"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VGB68_17745"
     CDS             complement(<1..741)
                     /locus_tag="VGB68_17745"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0051142.1"
                     /translation="MLEARNISINYGECEVLQNLSFALRNGRTLAVLGANGAGKTTLL
                     RALNGFLPVSKGEIRLEGKTLREFSRREIAQRMSVVAQENETKFPVTVLEFVLAGRFA
                     HGGVFGWESERDTGLAKSALEICDLKNYEARLMNRLSGGERQRVVFARALATGAKILL
                     LDEPTANLDLSHQALMFRLVRARCEKGEAAAVIVTHDLNLASEFADEILLLKNGRIAA
                     KGEPKEVLNAETLREVFAVEVLLDENPVS"
     gene            complement(948..1853)
                     /locus_tag="VGB68_17750"
     CDS             complement(948..1853)
                     /locus_tag="VGB68_17750"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY0051143.1"
                     /translation="MLSFFIAVSLGSESLSFFDLSDRQQTILYDIRLPRVLLGLATGA
                     SLAVAGAAFQSLLRNPLAEPYLLGVSNGAALGTMFAFIFFSQFEFARPTAAFLGASLA
                     TFTVYKMAQNRAGMNTERLVLSGVIVTTFLSSIIVLLTTLLDAAKLRSFTFWLLGDLS
                     QATKNSFYLAIIAAVLGTILLTATARSLNLMMLGERDAFDFGVEVGRTRLIVFATASA
                     LVGIAVASSGSVGYVGLVVPHIVRLLLGSDNRRVIPATALAGAIFVVLADTIARTAIA
                     PRELPVGAITALIGAPMFIYLLRKN"
     gene            complement(1969..>2390)
                     /locus_tag="VGB68_17755"
     CDS             complement(1969..>2390)
                     /locus_tag="VGB68_17755"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY0051144.1"
                     /translation="TRRKIDALRRRVADIEARTATAADVKVFVQISKEPLFTIGADSF
                     LTEIINRAGGFSATAQIPTAYPRFSKETALALQPEAIILSESDDNTEPNEVFKDSPAV
                     KNGRIFKINADLLSRPGPRIVDALEQMAKDLHPESFR"
BASE COUNT          588 a          707 c          605 g          490 t
ORIGIN      
        1 actgaccgga ttttcgtcca gcaaaacttc gacggcgaaa acctcacgta aagtttccgc
       61 gtttaaaacc tctttcggct cgcctttggc ggcaatgcgc ccgtttttca aaagcagaat
      121 ctcgtcggca aattccgaag ccagattcaa atcgtgcgtg acgataaccg ccgcggcttc
      181 gcctttttcg cagcgggcgc gcaccagccg aaacatcagc gcctgatgcg ataaatcgag
      241 attcgccgtc ggttcgtcca gcagcagaat tttcgctccc gtagccagcg cccgcgcgaa
      301 aacgacgcgc tggcgttcgc cgccggaaag ccggttcatc aggcgcgctt cgtaattttt
      361 caaatcgcag atttccagcg cgcttttcgc caagcccgtg tcgcgctcgc tttcccagcc
      421 gaacacgccg ccgtgcgcga atctgcccgc cagaacgaat tccagaaccg tgaccggaaa
      481 cttcgtttcg ttttcctgcg cgacgaccga cattcgctgc gcgatttcgc gccgcgaaaa
      541 ctcgcgcagc gtttttcctt cgagccgaat ttcgcctttc gaaaccggca aaaaaccgtt
      601 cagagcgcgc agcagagtag ttttgcccgc gccgtttgcg cccaaaacgg cgagcgtccg
      661 accattccgc aaagcgaaag agaggttttg caaaacctcg cactcgccgt aattgattga
      721 aatgtttcga gcttccaaca tttaattttc gccgcagagc gcacagagat ttttaagaaa
      781 acgaaaccgc agatcgacac ggatacacgc ggataagaaa gaaatttgaa gcaacggatt
      841 aacacggcag aacgcggatt tatttttatc ctgtaaatcc cgttttaatc tgcgtttatc
      901 tgcgttcatt cgtggtttca ctctcttttt ttcgtgtcct gcggcggcta atttttccgc
      961 aataaataaa taaacatcgg cgcgccgatg agcgccgtta tcgcgccgac cggcaattcg
     1021 cgcggcgcaa tcgccgtccg cgcaatcgtg tccgccagca cgacgaaaat cgcgcccgcc
     1081 agagcggtcg ccggaatcac gcggcgattg tcgctgccga gaagaagccg cacgatatgc
     1141 ggcacgacca gaccgacata gccgaccgaa ccgctcgaag cgacggcgat tccgaccagc
     1201 gcgctcgccg tcgcaaaaac aatcaggcgc gtgcgcccga cttcgacgcc gaaatcgaaa
     1261 gcgtcgcgct cgccgagcat cattaaattt aaagagcgcg ccgtcgccgt taaaagtatc
     1321 gtccccagaa cggcggcgat aatcgccaga taaaacgaat ttttcgtcgc ctgcgacaaa
     1381 tcgcccaaaa gccagaacgt aaagcttcgg agctttgccg cgtccagcag agtcgtcagc
     1441 aaaacgatta tcgaagacaa aaacgtcgtc acgatgacgc ccgacaaaac cagtctttcc
     1501 gtgttcattc cggcgcggtt ctgcgccatt ttatacaccg tgaaagtcgc cagactcgcg
     1561 cctaaaaacg ccgccgtcgg gcgcgcaaat tcaaactggc tgaaaaaaat aaacgcgaac
     1621 atcgttccca gcgccgcgcc gttcgagacg ccgagcaggt aaggctcagc caacggattt
     1681 cggagcagcg attgaaatgc tgcgcccgcg accgcgaggc ttgcgcccgt cgccaagccg
     1741 agcaaaacgc gcggcaggcg aatgtcgtac aaaatcgtct gctgccggtc gcttaaatca
     1801 aagaacgaaa ggctttcgct gccgagcgag acggctataa aaaaggacag caaaagcaaa
     1861 ccgccgaaga caagaggcac agagataaat ttataaaaaa atccgtgttt atctgtgtca
     1921 atcactggtt tcatattcta aaaatctctg catcggcggc gctgaaaatc atcgaaaact
     1981 ttcgggatgc aaatccttcg ccatctgctc cagcgcatcg acaatgcgcg gaccgggacg
     2041 cgacagcaaa tcggcgttga ttttaaaaat ccgcccgttt ttcaccgccg gagaatcttt
     2101 gaaaacttcg ttcggctcgg tattgtcatc gctctccgac aaaattatcg cttccggctg
     2161 cagggcgagc gcggtctcct tgctgaatcg cgggtaagcg gtcggaatct gagccgtcgc
     2221 ggaaaagccg cccgcgcgat ttataatttc ggtcaaaaaa gaatccgcgc cgatggtgaa
     2281 aagcggttct ttcgagattt gcacgaagac ttttacatct gccgccgtcg cggttcgcgc
     2341 ctcgatatcg gcgacccggc gacgcagcgc gtcgatcttc cggcgcgttt
//
LOCUS       DASWNE010000906         2251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_241895, whole genome shotgun sequence.
ACCESSION   DASWNE010000906 DASWNE010000000
VERSION     DASWNE010000906.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241895"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VGB68_17760"
     CDS             <1..342
                     /locus_tag="VGB68_17760"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0051145.1"
                     /translation="QEKTINKEAMSALVAYNWQGNIRELQNAVERAFILSGAEEITVE
                     NLPPKILAFSQNSFEMRDAESRRPTLEEMERRYILEILSSVDEDKTEAANILGIDLST
                     LYRKLKRYEEI"
     gene            complement(370..903)
                     /locus_tag="VGB68_17765"
     CDS             complement(370..903)
                     /locus_tag="VGB68_17765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051146.1"
                     /translation="MNFFPKASIKSKSIWFLLLSIILSVSCGNQTEPNANGRTNSNAA
                     NIANNENCSVSNDDIAALGNQVLLPLVPEEVSFCEENVKDSGDKKLTAVLKFTEEDSK
                     NLVAQAEKHRPAEAVEIGTEQWFPLELSAQSRLSGNESLKGNAYAPTDFLQLPYKTGK
                     LVKIENSNYFILELATN"
     gene            1004..1825
                     /locus_tag="VGB68_17770"
     CDS             1004..1825
                     /locus_tag="VGB68_17770"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0051147.1"
                     /translation="MLIFRRIRAFIIRDFHLSLSYRVEFLIKILWILGITTTFYFISR
                     IFAGFPLARYQQWQNPLAAWLTGMAMLNYFLVGFSSLANAIRQEQMQGTLESVLLTPV
                     GVPTVIVSSSAWAYVEATFYSFLYLFFGWIFFNVQYKGNFLLALMFLLLTTLVLACLG
                     ILSASFTMAFKRGDPFAILLGAGTALFSGVFYPKEMLEDALGKTAGKVVSFINPSTHG
                     LDAIRGILIQGKAFAEVQSSFLALMSFLVVLLPFSLWVFSRAIRRAKREGSLIQY"
     gene            complement(1785..>2251)
                     /locus_tag="VGB68_17775"
     CDS             complement(1785..>2251)
                     /locus_tag="VGB68_17775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051148.1"
                     /translation="QLLTAHSQPLIEHSQPLNEDSQLLIEHSHVTDEDSHVTDEHSQP
                     TDEHSQPTDEHSQLTDGDSQLTDEDSQLTDTHSQVNGEHSQLNDEHSHVTGTDSHVND
                     EHSQVNDEDSQLNGEHSQLNDADSHVTGEDSQYKTARNRLNTELNCLRAWRV"
BASE COUNT          483 a          523 c          560 g          685 t
ORIGIN      
        1 caggaaaaaa ctattaacaa agaagcgatg tcggcgctgg tcgcgtataa ctggcagggc
       61 aatatccgcg aattacaaaa tgcggtcgag cgcgcattta ttctttccgg cgcggaagaa
      121 atcacggtcg aaaacctgcc gccgaaaatt ctcgcttttt cgcaaaactc atttgaaatg
      181 cgcgacgccg aaagccgtcg cccgacgctc gaagaaatgg aacggcgcta tattctggaa
      241 attctcagct ccgtcgacga agataaaacc gaagcggcga atattctggg catcgattta
      301 tcgacgcttt accgcaagct gaaacgctac gaggaaattt gaggtcggaa gcaagtggaa
      361 aattatcgtt taattcgtcg ccaattcaag aataaaataa ttcgagtttt caattttgac
      421 gagttttccc gttttgtacg gaagctgcag aaaatcggtc ggagcgtaag cgtttccctt
      481 taaagactcg tttccggaaa gccgcgattg agcgctcagc tccaacggga accattgttc
      541 cgtcccgatt tcaacggctt ccgccggacg atgtttttcc gcctgcgcga ccagattttt
      601 tgaatcttcc tcggtaaact tcaaaacggc ggtcagtttt ttatcgccgg aatctttcac
      661 gttctcttcg caaaatgaga cttcttcggg cacgagaggc aaaagcacct gattgccgag
      721 cgccgcgatg tcgtcgttgg agacggagca attttcgttg ttggcaatgt tggcggcgtt
      781 cgagtttgtc ctgccgttcg cgttcggttc ggtttgattg ccgcaggaaa ccgataaaat
      841 gattgacaat aataaaaacc agattgattt tgatttgatg gaagctttcg ggaaaaaatt
      901 catacggcaa ttttattttg attagcgttt gtttgcaatc aaacaatttg tcctgcgatt
      961 attaatacgt tttaaattaa gattggttcc cgggctttat tcgatgctta tttttcgccg
     1021 cattcgcgcc tttatcatcc gcgattttca tttgtcgctg tcttaccgcg tcgaattcct
     1081 gattaaaatc ctctggattt taggcatcac gacgacgttt tattttattt cacgaatctt
     1141 tgccggcttt ccgctcgccc gataccagca gtggcagaac ccgctggcgg cgtggctgac
     1201 gggaatggcg atgctcaatt attttctggt cggcttttcc agcctcgcca acgccatccg
     1261 gcaggaacag atgcagggca cgctcgaaag cgttctgctg acgccggtcg gcgttccgac
     1321 cgttatcgtc tcgagctcgg cgtgggctta tgtcgaagcg acgttttatt catttttata
     1381 tttgtttttc ggctggattt ttttcaacgt gcaatacaaa ggcaattttc tgctggcgct
     1441 gatgtttctg cttttgacga ccctggttct ggcgtgtctc ggaattttat cggcgagctt
     1501 tacgatggct ttcaaacgcg gcgacccgtt tgcgattctt ttaggcgcgg gcacggcgct
     1561 tttttccggc gtcttttacc cgaaagaaat gctcgaggac gcgctcggca aaaccgccgg
     1621 caaagtcgtt tcgttcatta atccctcgac gcacggtctg gacgcgattc gcggcatttt
     1681 gattcaaggc aaagctttcg cggaagtgca aagctctttt ctggcgctga tgagctttct
     1741 cgtcgtcctg cttccctttt cgctctgggt tttctcgcgc gccatcagac gcgccaagcg
     1801 cgaaggcagt ttaattcagt attaagtcga tttcgagcgg ttttatattg tgaatcttcg
     1861 ccggtcacgt gtgaatccgc gtcgttcagt tgtgaatgtt caccgttcag ttgtgaatct
     1921 tcgtcgttca cctgtgaatg ttcatcgttc acgtgtgaat ctgtgccggt cacgtgtgaa
     1981 tgttcgtcgt tcagttgtga atgttcgccg ttcacttgtg aatgtgtatc tgtcagttgt
     2041 gaatcttcat ctgtcagttg tgaatctcca tctgtcagtt gtgaatgttc gtctgttggc
     2101 tgtgaatgtt catctgtcgg ctgtgaatgt tcgtctgtca cgtgtgaatc ttcatctgtc
     2161 acgtgtgaat gttcaatcaa cagttgtgaa tcttcgttca acggttgtga atgttcaatc
     2221 aacggttgtg aatgcgcggt caacagttgt g
//
LOCUS       DASWNE010000907         1875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_241915, whole genome shotgun sequence.
ACCESSION   DASWNE010000907 DASWNE010000000
VERSION     DASWNE010000907.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241915"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VGB68_17780"
     CDS             complement(<1..706)
                     /locus_tag="VGB68_17780"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEY0051149.1"
                     /translation="MKEASVLQLEPKIHTRREARVFRAKAFLLQLKRNAENLVNSDIR
                     RFPQTFDLTGQLIVGESKTALWTEKDRAEMPLMAGKIHNLRLAIKKLNGIEIPANQIF
                     SFWKHVRRATRRRGFVRGRELREGCIIPNVGGGLCQISNALYDAALQANFEIVERHAH
                     TQIVQGSLAEKGRDATVFWNYVDLRFRSGGAFRIEAELTATHLIVRFRAKEKKQIPVR
                     LKTRKEFSILNNEPNSC"
     gene            complement(897..1472)
                     /locus_tag="VGB68_17785"
     CDS             complement(897..1472)
                     /locus_tag="VGB68_17785"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEY0051150.1"
                     /translation="MSIATKAGDRGKTALIGGERVSKADLRVEAYGTIDELGAQMAFA
                     RSICGDAGIDELTKSIQRELFMVSGSLANPNFADAPKPYLTPEMINRLTTEVTRIEKM
                     EGILTDWSLPGDVAAAAAFDIARTVCRRAERCVVRLQETGETVDAQVITYLNRLSDLL
                     WLVGRLLELQAGVDSRLRDDEHEGNKWSRAW"
     gene            complement(1627..>1875)
                     /locus_tag="VGB68_17790"
     CDS             complement(1627..>1875)
                     /locus_tag="VGB68_17790"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY0051151.1"
                     /translation="ALKAKDGKNIWLSGGGGLAKSFLDEDLVDEIYLGVTPLLLGSGL
                     PLFPELIREVPLRFVSCNVCRHKKEDNAMLELVYEVKR"
BASE COUNT          431 a          517 c          452 g          475 t
ORIGIN      
        1 tacaactgtt cggctcatta ttaagaatcg aaaactcttt tcgcgtcttc agccgaaccg
       61 gaatttgttt cttttccttc gctctgaaac gaacaatcaa atgcgtcgcg gtcagctcgg
      121 cttcgatgcg aaaagctccg cccgagcgaa aacgtaaatc gacgtaattc cagaaaacgg
      181 tcgcgtcgcg tcctttttcc gccagcgagc cctgcacgat ttgcgtgtgc gcgtggcgct
      241 cgacgatttc gaaattcgcc tgcaaagccg cgtcgtagag cgcgttggaa atctgacaca
      301 aaccgccgcc gacgttcggg ataatgcagc cttcgcgcag ctcgcgcccg cgcacgaaac
      361 ctctgcgccg cgtcgcgcgc ctgacgtgtt tccagaaact gaaaatctga tttgccggaa
      421 tttcgatgcc gttcaatttt ttaatcgcca gccgcagatt atgaatcttg cccgccatca
      481 gaggcatttc cgcgcggtct ttctccgtcc acagcgcggt tttcgattcg ccgacgatca
      541 attgaccggt caaatcaaaa gtttgcggaa aacgcctgat atcggaattg acgagatttt
      601 ccgcgttgcg cttaagctgc aataaaaaag ctttggcgcg aaaaacgcga gcctcgcggc
      661 gggtatgaat tttcggttcg agttgtaaaa cggatgcttc tttcattttc ggatttttgc
      721 tgtgagtatg aacgcataaa atcttgagtt ttgcaattat ccggagagat atttttataa
      781 aaaagccttt aaaaattcag gattcaggat tcaggattca ggattgaatc cgatttccaa
      841 tcctgaattc tcaattttga atcttgaatc ctattttgca ctttgcattc aaatacttac
      901 cacgcgcgcg accatttgtt tccttcgtgc tcgtcgtctc ttagccggga atcgacgccc
      961 gcttgcagtt ccagtagtcg cccgacgagc cagagcaaat ccgataaacg attcagataa
     1021 gtgatgacct gcgcgtcgac cgtttcgccc gtttcctgca ggcgcacgac gcagcgttcg
     1081 gctcggcggc agacggttcg ggcgatgtcg aaagcggcgg cggcggcgac atcgccgggc
     1141 aggctccaat ccgtcaggat gccttccatt ttctcgatgc gcgtcacttc ggtcgtcagg
     1201 cgatttatca tttccggcgt cagatacggc ttcggcgcgt ccgcgaaatt cggattcgcc
     1261 aacgagccgg aaaccataaa caattcgcgc tggattgatt tcgtcagctc atcaatgcca
     1321 gcgtcgccgc agatggagcg cgcaaacgcc atctgcgcgc caagctcgtc aatcgtgccg
     1381 taggcttcga cgcgcaaatc cgcttttgaa acccgctcgc cgccgattaa agcggttttt
     1441 ccccggtcgc ccgctttggt tgcaatgctc atatttcaaa attaatttta cggtcgtaaa
     1501 aaagcaaata agaattaaag attcggcgcg gcgaaaaggc gaaaaggcgg agagcgcctt
     1561 ccgattttat cgctcgtcgt taaagaacac gctttatcgc cgcccgtctt tccgctttta
     1621 tctgctttat cttttcactt cgtaaaccag ttcgagcatc gcgttgtctt cttttttgtg
     1681 cctgcaaacg ttgcaggaaa caaatcgaag cggaacttcc cgaatcaatt cgggaaagag
     1741 cggcaagccc gaaccaagca ggagcggcgt cacgcccaga taaatctcgt ccaccaaatc
     1801 ttcatccaga aagcttttcg ccagcccgcc gccgcccgaa agccagatgt ttttgccgtc
     1861 tttcgctttc agagc
//
LOCUS       DASWNE010000908         4404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_30027, whole genome shotgun sequence.
ACCESSION   DASWNE010000908 DASWNE010000000
VERSION     DASWNE010000908.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30027"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /locus_tag="VGB68_17795"
     CDS             complement(<1..1014)
                     /locus_tag="VGB68_17795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051152.1"
                     /translation="MKKTTLVIILAAALGLSNATGANAQTEQLPPPPKPKPTAEPTPR
                     LSTVLAQNLEQMKSVDIPRERREQAYAKLLEGQRYLWMMSRQRSQSAMIAGGNLARQS
                     LQKAVELDPTLAEGYTALSELILTTPPNDIEEAILLASLAAKVNANSYGARRILARLY
                     TIKSRLNGGVLDVNWAQRAIEEWKEIARLDPRSAEAWAFLSEFYNRTDKNSERIDALE
                     KWRSSATPLETRFYRTVMGTQEDLSPESASLKLGSALIKAERSREAVEILSRAVAENP
                     ENSEAIDLLREAVENADGASTAKAIEALQQAVYANPANLVLVELLAGVQSRLGKTGDA
                     VRTL"
     gene            complement(1027..1512)
                     /locus_tag="VGB68_17800"
     CDS             complement(1027..1512)
                     /locus_tag="VGB68_17800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051153.1"
                     /translation="MNETSPSINAQLLEKRYRTSLIAVCVQILIMLALTVVAWFVITY
                     SNNDISEQAGVALWGTILLIAAGSFVLRRMLFSWERLKSVALLKGIPGLLDSLQANTI
                     LLGALGEVVAIMGFLVATLSGNKFEMLRAGAISLIVFLVNFPRKSVWQKILAGAEKVL
                     S"
     gene            complement(1574..3103)
                     /gene="bamD"
                     /locus_tag="VGB68_17805"
     CDS             complement(1574..3103)
                     /gene="bamD"
                     /locus_tag="VGB68_17805"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEY0051154.1"
                     /translation="MFFRKYLLSILSLFLILSFVSANFAQRTDGTPAQRLEVLSQKLN
                     TMRRSVSGALSTLKDESKDDKTKKEDKTSVETPMARLKSLEKEISGANSELNNLRGKI
                     DRTEKYEISDIEQLEATVSALQARVDSTLLETAKERANPTSPVGSKREIKEKTKKILW
                     LIPYGKERDEYEELIGNVVPGRDRELFIVATREVRKNNNEVGRLLFQTIITTYPDSPY
                     LPMAKLAIADSFYLEGSTSSLIQAAAAYQDWLTFFPTHPLACRVMLKIAESEMRQIGL
                     PDREITRAKRAEQRLRAFLQQRQCADSPLRGEVDQRLVEVQDNLGLHNLNIANYYYTL
                     SVNQKKGGLKGAQSRYREIMDKYPQFSYMDEALYKIAVTYQIEEETDEAAKYFQRLIR
                     DFPNSEFLEKAKEQLQLMGATIPEPNPARKNVMPPKKDGFFDNFKNQFLGIYPLTIDK
                     NGVLMTNDFDKSKFEMIDQIIENQGDIAASQIPKALTTVIKTTQAQPQSLTQPQPQSQ
                     K"
     gene            complement(3235..3897)
                     /gene="rpe"
                     /locus_tag="VGB68_17810"
     CDS             complement(3235..3897)
                     /gene="rpe"
                     /locus_tag="VGB68_17810"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEY0051155.1"
                     /translation="MIEIAPSILSADFSRLGEEIEAIEAGGASVLHFDVMDGRFVPNI
                     TIGLPVLKSVRKITKLKIDAHLMIVEPSRYAVEFVEAGANMVSVHVEADVHLQRTLTA
                     IREAGAEAGIAINPATPLVALEEALPFADFVLLMSVNPGFGGQKFIHTSLDKLRRLRM
                     MIDARGLKTRIEIDGGIDAGNIGKIAEAGAEIVVAGSAVFGGGKPTEAVKELIDKSTV
                     WV"
     gene            complement(3945..>4404)
                     /locus_tag="VGB68_17815"
     CDS             complement(3945..>4404)
                     /locus_tag="VGB68_17815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051156.1"
                     /translation="SYRYSQENPNTVIEQAPKAGDTVKTGHQILVVVAQQDPEGTEAP
                     ATLKKDEKDEVESLSNSPDKPKATNKNANAKKPEKTRDVISSKANKNSNSGNSGSNSA
                     NSNSKSNSNGADNKNSSTPATNKSPNPAKTPAVKPDKPPAAGDTRNRRTP"
BASE COUNT          921 a         1195 c         1111 g         1177 t
ORIGIN      
        1 cagagtcctg accgcgtcgc cggtttttcc taaacgcgac tgaacgcccg ctaaaagctc
       61 gaccagaacc agattggcgg gattggcgta aaccgcctgc tgcaaggctt cgatggcttt
      121 ggcggtcgat gcgccgtcgg cgttttcgac ggcttcgcgc agcaaatcaa ttgcttcgct
      181 gttttccgga ttctcagcca ccgcgcggct taagatttcc accgcctcgc gcgagcgttc
      241 ggctttaatc agcgccgagc cgagcttgag cgatgcgctt tccggcgaaa ggtcttcctg
      301 cgttcccatc accgtgcggt aaaatctggt ttccagcggc gtcgccgatg accgccattt
      361 ttccagcgcg tcgatgcgct cgctgttttt atcggttcga ttgtaaaatt cgcttaaaaa
      421 agcccacgct tcggcgctgc gcgggtcgag gcgggcgatt tccttccatt cttcgatggc
      481 tctctgcgcc cagttgacgt ctaaaactcc gccgttcaga cgacttttga ttgtgtagag
      541 ccgcgccaga atacggcgcg cgccgtaact gttggcgttt actttcgcgg cgagactcgc
      601 cagcagaatc gcttcctcga tgtcgttcgg cggcgtggtc aggataagct cggataaagc
      661 cgtataacct tcagctaaag tcgggtcgag ctcgacggct ttttgcagcg attgtctggc
      721 aagatttccg cccgcaatca tcgccgactg cgagcgctgg cggctcatca tccacaggta
      781 acgctgacct tccagcaatt tggcgtaagc ctgctcgcga cgctcgcgcg gaatatcgac
      841 tgacttcatt tgttcgaggt tttgcgccag cacggttgaa agtctgggcg tcggctctgc
      901 cgtcggcttg ggcttcggcg gcggcggcag ttgttccgtt tgagcgttcg cgcctgtggc
      961 gttcgataat ccgagagccg ccgccaaaat tattacgagc gtcgtttttt tcattaataa
     1021 aaccccttaa cttaaaacct tctccgcgcc ggcgagaatt ttttgccaga ccgatttgcg
     1081 cgggaaattt accagaaaga ctatcagcga aatcgctccg gcgcgcagca tctcaaattt
     1141 attgccgctc aaggtcgcca ccagaaagcc cataatcgcc acgacctcgc cgagcgcgcc
     1201 gagcagaatc gtattcgcct gcaagctgtc cagaagcccc ggaattccct tcagcagcgc
     1261 gaccgatttc agtctttccc acgaaaagag cattcgccgc agaacgaacg agccggcggc
     1321 aatcagcaga atcgtgcccc acaaagcaac gcccgcctgc tcggaaatgt cgttgtttga
     1381 ataagttatg acaaaccagg cgacgaccgt caaagccagc attatcagga tttgaacgca
     1441 gacggctatc aatgaggttc gataacgctt ttcgagcagc tgagcgttaa ttgaaggcga
     1501 agtttcgttc attatcgaca aaaaaagaca gcgagcattt tttataataa cactcgctgt
     1561 ccgaaattac cgtttatttt tgcgactgcg gctgcggctg tgtcagcgat tgcggctgcg
     1621 cctgtgtggt tttaatcacc gtcgtcaaag ctttcggaat ctgcgacgcg gcgatgtcgc
     1681 cttgattttc gataatctgg tcgatcatct cgaatttcga tttgtcgaaa tcattggtca
     1741 tcaaaacgcc gtttttatca atcgtcagcg gataaattcc caaaaattga tttttgaaat
     1801 tatcaaaaaa gccgtctttc ttcggcggca ttacattctt tcgcgccgga ttcggctcgg
     1861 gaatggtcgc gcccatcaat tgcagctgtt ctttcgcctt ttccagaaat tcgctgttcg
     1921 gaaaatcgcg tatcaggcgt tgaaaatact tcgccgcttc gtccgtttcc tcttcaatct
     1981 ggtaagtcac ggcgatttta taaagcgcct cgtccatata gctgaattgc ggatatttat
     2041 ccataatttc gcgataacgc gactgcgcgc ctttcaagcc gccttttttc tggttgaccg
     2101 aaagcgtgta atagtaattg gcgatgttca aattgtgaag tccgagatta tcctgcactt
     2161 cgaccagacg ctggtcgacc tcgccgcgca agggcgaatc ggcacactgg cgctgctgga
     2221 gaaacgctct cagacgctgt tcggcgcgct tggcgcgcgt aatctcgcgg tcgggcaaac
     2281 cgatttgacg catttccgat tcggcgattt tcagcatcac gcggcaagcc agcgggtgcg
     2341 tcggaaaaaa cgtcagccaa tcctgatacg ccgccgccgc ctgaatcaaa gagctggtcg
     2401 agccttccag ataaaacgaa tcggcaatcg ccagcttcgc catcggcagg taaggcgaat
     2461 cgggataagt tgtgataatc gtctggaaaa gaagccgccc gacctcgtta ttgtttttac
     2521 gaacttcgcg cgtcgccacg ataaaaagct cgcggtcgcg tccgggcacg acgtttccga
     2581 ttaattcttc gtattcgtcg cgctctttgc cgtaaggaat cagccacaga atttttttcg
     2641 ttttttcctt gatttcgcgt ttgctgccga cgggcgaggt cgggttggcg cgctctttcg
     2701 ccgtttccag aagcgtgctg tcgacgcgcg cctgcagggc ggaaaccgtt gcttccagct
     2761 gctcgatgtc gcttatctcg tatttttccg tgcggtcgat tttgccgcgc agattgttga
     2821 gctcggagtt tgcgccggaa atctcttttt cgagcgattt caaacgcgcc atcggcgttt
     2881 cgaccgacgt tttgtcttct tttttcgtct tatcgtcttt gctttcatct ttcaaagtag
     2941 agagcgcgcc gctcaccgag cgccgcatcg tgttgagctt ctggctcaaa acttcgaggc
     3001 gctgagcggg cgttccgtcg gttctttgcg caaaatttgc cgagacaaag ctcagaatta
     3061 aaaacaagct taaaatagat aataaatatt tacggaaaaa cataattctt ctccaaaagg
     3121 atgactcgaa ttttagattt acgattggaa ccggaattcg gctcgcgccg gataaattaa
     3181 aaatttccct tgcgaaatcg caaatctaaa cctaaaagct aaaatcaaaa attattaaac
     3241 ccaaacagtt gatttatcaa tcaattcctt aacggcttcg gtcggcttgc cgccgccgaa
     3301 aacggctgag ccggcgacta caatctcggc gcccgcttcc gcgattttgc caatattgcc
     3361 ggcgtcgatg ccgccgtcaa tctcgatgcg cgtttttaat ccgcgcgcgt caatcatcat
     3421 tcgcaggcgg cgcagtttat ccagcgaggt gtgaataaat ttttgcccgc cgaagcccgg
     3481 attgaccgac atcagcaaaa cgaaatcggc aaacggcagc gcttcttcca atgcgaccag
     3541 cggcgtcgcc ggattaatgg caattccggc ttccgcgccc gcttcgcgaa tcgccgtcag
     3601 agttctttgc agatgcacgt ccgcttcgac gtgaaccgaa accatattcg cgcccgcctc
     3661 gacgaattcg acggcgtatc ggctcggctc gacaatcatc agatgcgcgt cgattttcaa
     3721 tttggtaatt tttctgaccg attttaaaac gggcaagcca atcgtaatat tcgggacaaa
     3781 tcgaccgtcc atcacgtcaa aatgtaaaac gctcgcgccg cccgcctcga ttgcctcgat
     3841 ttcctctccg agccgcgaaa aatcagccga aagaattgat ggagctattt caatcattta
     3901 tttgttgctc aaaatttatt atcggttgct tttttacaaa aactctacgg cgtgcgacgg
     3961 ttgcgcgtgt ctccggctgc cggcggttta tcgggtttga cggcaggcgt ttttgccgga
     4021 ttcggcgatt tattcgtcgc cggcgtcgag ctgtttttat tatcggcgcc gtttgaatta
     4081 ctctttgaat tactgttcgc cgaattgctg ccggagtttc cgctgttcga gtttttattc
     4141 gctttactgg aaattacgtc gcgcgttttt tccggcttct tggcgttcgc gtttttattt
     4201 gtcgctttcg gcttgtcggg gctgttgctt aaactctcga cctcgtcttt ttcgtctttt
     4261 ttaagcgtcg cgggcgcttc ggttccttcc gggtcctgct gcgcgacgac gactaaaatc
     4321 tggtgtccgg ttttcacggt atcgcctgct ttcggcgctt gctcgataac ggtgttcggg
     4381 ttttcctgac tgtagcggta cgag
//
LOCUS       DASWNE010000909         5802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_666449, whole genome shotgun sequence.
ACCESSION   DASWNE010000909 DASWNE010000000
VERSION     DASWNE010000909.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5802
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666449"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..1062)
                     /locus_tag="VGB68_17820"
     CDS             complement(211..1062)
                     /locus_tag="VGB68_17820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051157.1"
                     /translation="MKKFAIILLLLGLSVSVFSQTKSRKPSKKATKSAPVVKTTPKPQ
                     AVEPVEADAPQKKNSRDESTQTVTENKAPQKKNQAEKPNANFVKLEPKYNYYYEFTQK
                     EFLVRHIVIEHDENGKGKITFEKKDFDESISDPVQLSPASLEKIKALFQTLNFVDSTE
                     AYQSAERNYGHLGTMKIRRKSEGKERTVTFNWTENKDARALAEEYRKIAQQYVWQFDI
                     SVARENQPLEAPGLMDALESYLKRNEISDPPQMLPLLKELSNDERIPLMARNHATKII
                     KQIEKPK"
     gene            complement(1069..4878)
                     /gene="smc"
                     /locus_tag="VGB68_17825"
     CDS             complement(1069..4878)
                     /gene="smc"
                     /locus_tag="VGB68_17825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEY0051158.1"
                     /translation="MFRLQRLEITGFKSFADYTEVIFTGDGITAVVGPNGCGKSNVSE
                     SIAWVLGEQRAKALRGGEMQDVIFQGTSKRNPSGMAEVVLHLVRDEEAFFEDDEHELG
                     DIDEALSDIDERAVQIEDFEPEAEEVSANGNGHQENGFDAEAAEVVAAETGAALTTQT
                     KIKTKRHWRPRRLALDFAPGESVSVTRRLYRSGESEYLLNGKSCRLRDIQDLFAGTGL
                     SGAHYAIIEQGRIGQILSAKPSDRRTLIEEAAGISKFRTRQRAAETRLESAKTNLRRI
                     SDIVDEIEKQANSLRRQASKTRRFKILKEELRITLRRVFAAEGSYLTELVGELEINLD
                     EAVKTERELFARVAEKDQAFRAATRKAREAEEELSDLRAAHAENALQRDRAERDLQYK
                     QEQVANLQTRAEVLKGEIEATGQRLKLVKSETERLEKDERRERAEAEKHELALRAAEN
                     KYQEKIGELRNIETELEKTRAEVFQHTTAFERLSEIGRQHESTIERLTERAEGLKREG
                     VRAAETLAEHLAESKKLETELAKAHENQAKLQDEKRVLSGAAGEAREILKTAEKELSN
                     WREQFSRTRHRLESLQELEQRRAIYAPTVQKLFAEEKKIGVKFLGTLADKLNVEAEAE
                     KAVENLFGAFLQTVLVESDADARKTLAYLNENKLGRLGVLVFSAKPQNGKGAKENKAQ
                     RAKDEGQKVYDFLGVDGDFAKLLAEVFPREASAELVENLEKVKSKPEQIFVDSDGDLM
                     IGGRLFVGGKANASEKNSSLLAFRRELRELESAGEKLKKEIEKAEKETEKARRNLAGK
                     EDKIVDLQSFIVKFEREVLSQEIQARTLKQEIERAERHKRLVAEETKQIEKELEEIRA
                     KRAETGTNAGKAEKARIAAAEKLENLARNLSQARQKVESENADLNEKRTAAGIAAERR
                     RSVSASLRRIEAEATELESRLARQTLEINENEAGYENLTALISEIARKIESAKETEGA
                     ENEQLSEASANLKNARENADAMSAELAELNKRSAEARNARAELEVRQAESLTRLKNLN
                     EICSHELGQSLVELVETVEIAEDFDLDASRRQTDEMRERIESFGAVNMLALEELSETE
                     ERLLFLTSQRQDIIDGISAAEEALREIKQRSRERFRHAFEAINEYFREFFLELFGGGR
                     GEMTLLDAEDVLESGIEIVAQPPGKRLQNILLLSGGEKAMTAIALVMAIFKYRPSPFC
                     LLDEVDAPLDDANVGRFVNKIAEMSEKTQFIVITHNKRTMESARALYGVTMQEAGVSK
                     VVSVRFE"
     gene            5572..5742
                     /locus_tag="VGB68_17830"
     CDS             5572..5742
                     /locus_tag="VGB68_17830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051159.1"
                     /translation="MREYIAILNFADIDNSADARKFADSQKFARASLAGNPKYAGQRT
                     GFAGTKQPNADA"
BASE COUNT         1178 a         1548 c         1406 g         1670 t
ORIGIN      
        1 caaaggaatc ttaacgccag attctacgaa atcggcgatt tccaccgtcc gcaatctctc
       61 gcagcttgat ttttcggcga caatgccgcg aatcggcgac gacggcacga tttcagccag
      121 gtaaaaaata aagctaaata agaattaaaa aggcaaaagt aaagatttta ttccttactt
      181 ttgccttttt aatttttact tttctcttat ctatttcggt ttctcgattt gcttgattat
      241 tttcgtcgcg tgattgcgcg ccattagcgg aatgcgctcg tcgttggaga gctcttttaa
      301 aagcggaagc atttgcggcg ggtcggaaat ttcgtttctt ttcagatacg attccagagc
      361 gtccatcaaa ccgggcgctt cgagcggctg attttctcgc gcgaccgata tatcaaactg
      421 ccagacgtat tgctgcgcga ttttgcgata ttcttccgcc agcgcccgcg catccttgtt
      481 ttccgtccag ttaaacgtga ccgttcgttc tttgccttcg ctcttacggc gaattttcat
      541 tgtgccgaga tgcccgtaat ttctttcggc ggattgataa gcttcggtcg aatcgacaaa
      601 attcagagtc tgaaacaaag ccttgatttt ttccagcgac gccggcgaaa gctgaaccgg
      661 gtcgcttatc gactcgtcaa aatctttctt ttcaaaagtg atttttccct tgccgttctc
      721 gtcgtgctcg ataacgatgt gacggacgag aaattctttt tgcgtaaatt cgtaataata
      781 attatatttc ggctccagct tgacgaaatt tgcattcggt ttttccgctt gatttttttt
      841 ctgcggagcc ttgttttccg ttacggtttg cgtcgattca tcgcgcgagt ttttcttctg
      901 cggagcatca gcctctaccg gctcgacggc ttgcggtttc ggcgtcgtct ttacgaccgg
      961 cgctgatttg gtcgctttct tagacggttt tctggatttc gtttgtgaaa aaacggaaac
     1021 gctcaagccc aataataaaa gtataattgc gaatttcttc attttaattt attcaaatct
     1081 gaccgaaacg acttttgaaa cgcccgcttc ctgcatcgtc acgccgtaga gcgcgcgcgc
     1141 cgattccatt gtccgcttgt tatgcgtgat aacgataaac tgcgtttttt cagacatttc
     1201 cgcgatttta ttaacaaagc gtccgacgtt tgcatcgtcg agcggcgcgt cgacttcgtc
     1261 gagcagacaa aacggcgacg gtcgatattt gaaaatcgcc atcaccagcg ctatcgccgt
     1321 catcgctttt tcgccccccg aaagaagcag aatattttgc aggcgcttgc cgggcggctg
     1381 ggcgacgatt tcgatgcccg attccagaac gtcttcggcg tcgagcagag tcatctcgcc
     1441 gcgaccgccg ccgaaaagct caaggaaaaa ctcgcgaaaa tattcgttta tcgcttcaaa
     1501 ggcgtgacga aaacgctcgc gcgagcgttg tttgatttcg cgcagggctt cttccgccgc
     1561 cgagatgccg tcgataatat cctgccgctg cgatgtcaaa aacaaaaggc gttcttcggt
     1621 ttcgctgagt tcttcgagag ccagcatatt gaccgcgccg aagctttcga tgcgctcacg
     1681 catctcatcg gtttgccgcc gcgacgcgtc caaatcaaaa tcttcggcaa tttcaaccgt
     1741 ttcgaccagt tcgaccagcg attgaccgag ttcgtgcgag cagatttcgt tcagattttt
     1801 caggcgcgtc aggctttcag cctggcgcac ttcgagttcg gcgcgcgcgt ttctcgcttc
     1861 ggcggagcgc ttgttcagct cggcgagctc ggcgctcatc gcgtcggcat tttcgcgcgc
     1921 gttttttaaa ttcgcgctcg cctcgcttaa ttgctcgttt tccgctcctt cagtctcttt
     1981 cgccgattcg attttgcgcg cgatttccga gattaaagcg gttaaatttt cataaccggc
     2041 ttcattttcg ttgatttcga gcgtctggcg cgccagacgc gattccagct cggtcgcctc
     2101 ggcttcgatg cggcgtaagg aagcggaaac cgaacggcgg cgctcggcgg cgatgccggc
     2161 ggcggttcgc ttttcgttca aatcggcgtt ttcgctttcg actttctgcc gcgcttggct
     2221 taaatttcgc gcgagatttt ccagtttttc cgcggcggca attcgcgctt tctcggcttt
     2281 tccggcgttc gttccggttt ccgctctttt ggcgcgaatt tcttccagtt ccttctcaat
     2341 ctgtttcgtt tcttcggcga cgaggcgttt gtgccgctcg gcgcgctcga tttcctgttt
     2401 caaagttcgc gcctgaattt cctgcgacaa aacctcgcgt tcgaatttga cgataaacga
     2461 ttgcaaatcg acgattttgt cttccttccc ggctaaattt cggcgggctt tttccgtttc
     2521 cttttcggct ttttcaattt ctttttttaa tttttcgccg gcgctttcca gctcgcgcag
     2581 ctcgcgcctg aaagccagca gcgacgaatt tttctcgctg gcatttgcct ttccgccgac
     2641 gaaaagtctt ccgccaatca tcaaatcgcc gtcggaatcg acgaaaatct gctccggttt
     2701 cgatttaact ttttccagat tttcaaccag ttcggcggaa gcttcgcgcg gaaaaacttc
     2761 ggcgagaagt ttagcgaaat cgccgtcgac gcctaaaaag tcatagactt tttgtccttc
     2821 gtccttcgcc ctttgtgctt tgttttcctt tgcgcccttg ccgttttgcg gcttcgcgct
     2881 aaaaaccaaa acgccgagcc tgccgagttt gttttcattc agatacgcga gcgttttccg
     2941 cgcgtccgcg tcggattcga ccagaacggt ttgcagaaat gcgccgaaaa ggttttcgac
     3001 cgctttttcg gcttccgctt cgacatttag cttgtcggct aaagttccga gaaatttaac
     3061 gccgattttc ttttcttcgg cgaaaagctt ctgcacggtc ggcgcgtaaa tcgcgcgccg
     3121 ctgctcaagc tcctgcaagc tttcgagccg atgccgcgtg cgcgaaaact gctcgcgcca
     3181 attcgataat tctttttcgg cggttttcaa aatttctctg gcttcgcccg ccgcgccgct
     3241 taaaacgcgc ttttcatcct gcagcttcgc ttgattttcg tgcgctttgg caagttcggt
     3301 ttccagtttt ttgctttcgg cgagatgctc ggcgagagtt tccgcggcgc gtacgccttc
     3361 gcgctttaag ccttcggcgc gttcggtcag gcgctcaatc gtgctttcgt gctggcgtcc
     3421 gatttccgaa agacgctcga aggcggtcgt gtgctggaaa acttcggcgc gcgtcttttc
     3481 aagctctgtt tcgatgtttc ttaattcgcc gattttttcc tgatatttgt tttccgcggc
     3541 gcgcagggcg agctcgtgtt tttcggcttc ggcgcgttcc ctgcgctcgt ctttttcgag
     3601 tctttccgtt tccgatttaa ctaatttgag ccgttgtccg gtcgcttcga tttcgccttt
     3661 taaaacttcg gcgcgcgttt gcagattggc aacctgctcc tgtttatact gcaaatcgcg
     3721 ctcggcgcgg tcgcgctgta aagcgttttc ggcgtgcgcg gcgcgcaaat cgctcaattc
     3781 ttcttcggct tcgcgcgctt tccgcgtcgc cgcccgaaac gcctggtctt tctccgcaac
     3841 tcgcgcgaaa agctcccgct cggttttgac ggcttcgtcg agattgattt ccagctcgcc
     3901 gaccagctcg gttaaataac tgccttcggc ggcgaaaacc cgtcgcagag taatgcgcag
     3961 ttcttctttt aagattttga aacgacgcgt tttagacgcc tggcggcgaa gcgagtttgc
     4021 ctgtttttcg atttcatcga cgatatcgga aatgcggcgc agatttgttt tcgccgattc
     4081 cagacgcgtt tcggcggcgc gctggcgcgt gcggaatttg gagattccgg cggcttcctc
     4141 gattaaagtg cggcggtcgg aaggcttggc ggaaagaatc tgcccgatgc gtccctgctc
     4201 gataatcgcg taatgcgcgc cgctcaagcc cgttccggca aacaaatcct gaatatcgcg
     4261 caggcggcag ctcttgccgt tcagaagata ttcgctttcg cccgaacgat aaagccggcg
     4321 cgtgacggaa accgattcgc ccggcgcgaa atccagcgcc aggcggcgcg gtcgccagtg
     4381 gcgtttcgtt tttatttttg tttgagtcgt taaagccgcg ccggtttcag cagcgacgac
     4441 ttcggcggct tcggcgtcaa aaccgttttc ctgatgaccg tttccgttgg cggagacctc
     4501 ttcggcttcc ggctcgaaat cttcgatttg aaccgcgcgc tcgtcaatat cgctcaaggc
     4561 ttcgtcgatg tcgccgagct cgtgttcgtc gtcttcaaaa aacgcttcct cgtcgcgcac
     4621 cagatgcagc acgacctcag ccattcccga aggattgcgc ttgctcgtgc cctgaaaaat
     4681 cacgtcctgc atttccccgc cgcgcagggc tttggcgcgc tgctcgccca gaacccacgc
     4741 gatcgattcg gagacgttcg atttgccgca gccgttcggt ccgacgacag ccgtaattcc
     4801 gtcgcccgta aaaattactt cggtgtaatc ggcgaatgat ttaaaacctg tgatttcgag
     4861 tcgttgaagt ctgaacatca aaaaatagcg ctattttgtg aatgaaaggc agattagcac
     4921 tatattttgt tgcaggcgcg aagtcaaatc aagagacagc cgcaaagatt tacaaaggac
     4981 gcaaaaattt cgtgtaatcg ttcaaataaa aatattgaca aaagaaatac tttctcataa
     5041 gatacatcta ccgacaacac gacaaatagg gggttgaatt gagcgcatta ttaattgatg
     5101 tgtttgttaa acctcatatg ttcagatgaa gataattttc taatttatct ttactgacaa
     5161 aactattttt aattatgttg tctattctac atcaatcaaa aagcgaaagt cggtttcctg
     5221 tgttggcagc gaatcggctt tttaatttgc gcttagatgc cggaatcgaa catcatccgc
     5281 agcggcaatt tctgacaaaa tttaaggata ttttaaagga gccaaaatga aaaaacttat
     5341 cgcgtatcgc tctatttgtg cttttctcat tgttttttgt ttcttaacaa atgcgcttcc
     5401 tcaaagagat ttaggaagca ttgttttccc tcctcgtcct ccgcaagatc cgccaatggg
     5461 acccaggacg acggtaagag atatacttcc gccgccgtca actagtatat tcggaattag
     5521 cagcactagt tcgaacagag ccgcccggcg ctcttcgcct tttgtggaca gttgcgagaa
     5581 tatatcgcca ttctcaactt tgccgacatt gacaactctg ccgacgctcg gaaatttgcc
     5641 gacagtcaga aatttgcccg cgcctcgctc gccggaaatc cgaaatacgc gggtcagagg
     5701 acaggcttcg ccggtacaaa acaaccgaac gcggacgcct gagccggcga cgagaacgag
     5761 atgggttgtt tatgtttcaa attaaagaaa gacctcatcg aa
//
LOCUS       DASWNE010000910         1581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_278109, whole genome shotgun sequence.
ACCESSION   DASWNE010000910 DASWNE010000000
VERSION     DASWNE010000910.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278109"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            273..>1581
                     /locus_tag="VGB68_17835"
     CDS             273..>1581
                     /locus_tag="VGB68_17835"
                     /inference="COORDINATES: protein motif:HMM:NF013276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HEY0051160.1"
                     /translation="MKLFWLIGAILLLAAAAAEAQPAKQNPKSEIRNPKSFDLRVAAD
                     GAGDVRTVQEAIDRVPENNKKRFVIFIKPGVYKEQIRIPAGKPYISLVGEKAETTKLT
                     FDLNNKAAGSTSASYSFYVGAHDFYAENITFENSFGTGSQAVAVLTEADRAVFKNCRF
                     LGWQDTLYAKNGRQYYENCYIEGHVDFIFGQAAAVFENCVIHSKGDGYIAAPMRFAAD
                     EPSGFVFVNSKLTGEKTGKGVYLGRPWRDYGRTVFLDTEMGAHIRPEGWHHWLPEREK
                     TAYFAEFNSKGAGAKPETRVKWARQLTKDEAKVFRPENFLKGADGWNPKKADGDWQQK
                     NAPDYKLVSWNRVLDQPPLWYAVDEATRIANQVVVYQRENGGWGKNVDMAAMLTEAER
                     EKLIKEKSSTDTTIDNNATTMQLRYLAKVVTAKNIETHKAAFLK"
BASE COUNT          420 a          425 c          419 g          317 t
ORIGIN      
        1 ttccacatcc gcgaaggaat gaaattgcag gtgcgcgtcg aggcgattaa cgtccgcaac
       61 tgggtttatc tgggcgacgg aatccagctc gcttcaaaca acgcgagctt cggactggtc
      121 ggcggacaga gaaatctgcc gcgcgacatc caaatcggcg gcagatttac cttctagctc
      181 ccgacaggaa aaagccggtc ttttatttaa cgcataagcc ctttgcggcg ctaaacctcg
      241 aagggcttgt gttgtcttta cagaggatga agatgaaact cttttggctg attggcgcga
      301 ttttattgct cgctgcggct gccgccgagg cgcagccggc gaaacaaaat ccgaaatccg
      361 aaatccgaaa tccgaaatcg tttgatttaa gggtcgccgc ggacggcgcg ggcgacgtta
      421 gaaccgtgca ggaagcaatc gaccgcgttc ccgaaaacaa taaaaaacgt tttgtcattt
      481 tcatcaaacc cggcgtttat aaagagcaaa tcagaattcc cgccggcaag ccgtatattt
      541 cgctggtcgg cgaaaaagcc gaaaccacaa aattgacttt tgatttaaac aataaagcag
      601 ccggctcgac ttcagccagc tacagctttt acgtcggcgc gcacgatttt tacgccgaaa
      661 acatcacgtt tgaaaactct ttcggcaccg gctcgcaagc cgtcgccgtg ctgacggaag
      721 ccgaccgcgc cgttttcaaa aactgccgtt ttctgggctg gcaggacacg ctctacgcca
      781 aaaacggtcg gcagtattac gaaaactgct atatcgaagg tcacgtcgat tttattttcg
      841 ggcaagccgc cgccgttttt gaaaattgcg tcattcattc caaaggcgac ggctacatcg
      901 ccgcgccgat gcgctttgcc gccgacgagc cgagcggttt cgtattcgtc aattcaaaac
      961 tgacgggcga gaaaaccggg aaaggcgtct atctcggacg cccctggcgc gattacggca
     1021 gaaccgtttt tctcgacacg gaaatgggcg cgcacattcg cccggaaggc tggcatcact
     1081 ggctgcccga acgcgaaaaa acggcgtatt tcgccgaatt taactccaaa ggcgcgggcg
     1141 caaaacctga aacgcgcgtc aaatgggcgc ggcagttaac caaagacgag gcaaaagttt
     1201 tccgtccgga aaactttctc aagggcgcgg acggttggaa tccgaaaaaa gcggacggcg
     1261 attggcaaca aaaaaatgcg cccgattaca agctcgtttc gtggaatcgg gttttagacc
     1321 agccgccgct ctggtatgcg gttgacgagg cgacgcgcat cgccaatcaa gtggtcgtct
     1381 atcagcgcga aaacggcggc tggggaaaaa acgtcgatat ggcggcgatg ctcaccgaag
     1441 ccgagcgcga aaagcttata aaggaaaaat cctcgaccga cacgaccatc gacaacaacg
     1501 cgacgacgat gcagcttcgt tatctcgcca aagtcgtcac cgccaaaaac atcgaaacgc
     1561 acaaagcggc ttttttgaaa g
//
LOCUS       DASWNE010000911         1893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_489289, whole genome shotgun sequence.
ACCESSION   DASWNE010000911 DASWNE010000000
VERSION     DASWNE010000911.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489289"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1674)
                     /locus_tag="VGB68_17840"
     CDS             complement(<1..1674)
                     /locus_tag="VGB68_17840"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEY0051161.1"
                     /translation="MILSFVSTSTASTLFYTAKDKKAVPQNRFQPSEKAWKWADKKLK
                     KMSLDEKIGQLVHIGINARFLSQDSNEFQELKRQVTENKIGGIIVFVGGVYETVHLVN
                     RMQELAETPLLISADFETGVAMRFEDAVNFPWNMAVAATGNPEFARRQGEIVARESKA
                     LGVMQIFAPVVDVNNNADNPVINVRSYGENPADVSRFAIAFMEGLQANGVLATAKHFP
                     GHGDTNVDSHRGLPVINFSRERLEQTEFAPFRELIKAGVGSVMISHISLPQIDAGIVR
                     PLKQSVKATYTESEIITEGTTTPATLSSNVITDILRKDMNFEGLIVTDAMDMSGLTLY
                     FNQEEAAVRAISAGNDILLKPANPDAAVRGLKEAARTGRLTEDRINQSVRKQLAWKYR
                     LGLEKNKITPIDAIDRIVSSSETRKLTAEIAESSITLVKNEENALPLSPAGDKKVFVL
                     IITNGENPNAAGTSFLRLLRQSFQKPQNIDFGIVGENTTAAEIKRIEDRMKRSEIVLA
                     GLYGRVRSGAKNSVGLPETGARVLRDLLQSDKQTIGISFGNPYLLKSFPE"
BASE COUNT          455 a          487 c          481 g          470 t
ORIGIN      
        1 ttcggggaag ctttttaaaa gatacgggtt gccgaagctg atgccgattg tctgcttgtc
       61 gctttgcagc aaatcgcgca ggacgcgcgc gcccgtctcc ggcagaccga ccgagttttt
      121 cgcgcccgaa cgaacgcgcc cgtaaagacc ggcgagaacg atttcgcttc gcttcatacg
      181 gtcttcaatc cgtttgattt ccgccgccgt cgtgttttcg ccgacgattc cgaaatcaat
      241 attctgcggt ttttgaaagc tctggcgcaa aagtcttaaa aaactcgttc ccgccgcgtt
      301 cggattttcg ccgttggtga taatcaaaac gaaaactttt ttatcgccgg cgggcgaaag
      361 cggcagcgcg ttttcttcgt ttttgacgag cgtaatcgag ctttcggcaa tctcggcggt
      421 caattttctg gtttcgctgc tcgaaacaat ccggtcaatg gcgtcaatcg gcgtgatttt
      481 atttttttcc agaccgagcc ggtatttcca agccagctgt ttgcggacgg attgattgat
      541 tctgtcttcg gtcaggcgac ccgtccgggc ggcttctttc aatccgcgca cggcggcgtc
      601 cgggttagcc ggtttcagca gaatgtcgtt gccggctgag atagcgcgga cggcggcttc
      661 ttcctggttg aaataaagcg tcaagccgct catatccatc gcgtcggtga cgataagccc
      721 ttcgaaattc atatctttgc gcagaatatc ggtgatgacg ttcgacgaaa gcgtggcggg
      781 cgtcgtcgtg ccctcggtga taatttccga ttcggtataa gtcgctttga cggattgttt
      841 gagcgggcgg acgattccgg cgtcaatctg cggcaggctg atgtgcgaaa tcatcaccga
      901 cccgacgccc gctttgatta attcgcgaaa cggcgcaaat tccgtctgtt ccagacgctc
      961 gcgcgaaaaa ttgatgaccg gcaagccgcg atgcgaatcg acgttcgtat cgccgtgacc
     1021 gggaaaatgc ttcgccgtcg ccagcactcc gttcgcctgc aagccttcca taaaagcaat
     1081 cgcaaaacgc gaaacgtccg ccggattctc gccgtacgag cggacgttga tgacgggatt
     1141 gtcggcgttg ttgttaacgt cgacgacggg tgcgaaaatc tgcatcacgc cgagcgcttt
     1201 cgattcgcgc gcgacgattt cgccctgacg acgcgcaaat tccggattgc ccgtcgccgc
     1261 gacagccata ttccacggaa aattaaccgc gtcttcaaaa cgcatcgcca cgccggtttc
     1321 aaaatcggcg gaaatcagaa gcggcgtttc ggcaagctcc tgcattcggt taaccagatg
     1381 cacggtttca taaacgccac cgacgaagac gataatgccg ccgattttgt tttccgtcac
     1441 ctgtcgcttt aattcctgaa actcgttgct gtcctgactt aaaaaacgcg cgttgatgcc
     1501 gatgtgtacg agctgaccga ttttttcgtc cagactcatt tttttgagtt ttttatccgc
     1561 ccatttccag gctttttccg aaggctgaaa gcgattttgc gggacggctt ttttatcttt
     1621 ggctgtataa aaaagcgtcg aggcagttga ggtcgagaca aagctcagaa tcaacaaaag
     1681 cgcgcaagaa aattttaaaa aagaaaaaag cataaatata aactgaaaag aaccggagct
     1741 atctgaaaat cgcgaagaac gcggagtgat tttcagacag ccggacaaag ctgcaaaatc
     1801 aaaataacgg aaaataaaaa atctattcca aaacaataaa cggttaattc gcacgcgaaa
     1861 cattttttgt ttcggaaaaa ataaaactcg cca
//
LOCUS       DASWNE010000912         3658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_562321, whole genome shotgun sequence.
ACCESSION   DASWNE010000912 DASWNE010000000
VERSION     DASWNE010000912.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562321"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..1137)
                     /locus_tag="VGB68_17845"
     CDS             complement(124..1137)
                     /locus_tag="VGB68_17845"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY0051162.1"
                     /translation="MFQTTARERLLNFLSNQKTGAENIRLEVLTPDASTREFFRIDWH
                     NQTAIACIYGESFVAVEHNYLDVTELFLESRLPVAEIFAFDEASGIIILEDFGDRILR
                     DVLKKTNDEAREDFLNEAIRLIARIQAATPKAFELNSIAAKLKFDEEKLGWELNFFKT
                     HYFESLKKQKLSGADERRLSEEFTEIARELETKASVLCHRDFHTANLMFDRQNRLRII
                     DHQDARLGSVTYDLVSLLLDRVSAPPSRAWVREKRLFFLSEREKLGLETIDPDVFAGE
                     FHLQTIQRCLKATGTFSYQSVFREKAHFISFIEPMLQIVWQACERLGRFPNLRRIIGD
                     ELN"
     gene            complement(1164..2165)
                     /locus_tag="VGB68_17850"
     CDS             complement(1164..2165)
                     /locus_tag="VGB68_17850"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEY0051163.1"
                     /translation="MKAMILAAGFGTRLFPLTIDRTKPAIPFLGKPLVGYVAEYVARY
                     GFRDVVVNLHHQPESVKNALGDGSRFGVNIHYTIEAPEILGTSGALDNARHLLEDETF
                     LIINGKIITDIDIAAAVETHRKSGALATMVLKPNTRRERFTEVIIENGLIKSFGNFPE
                     PSTGAEVENRTSNIEHPLMFTGIHILEPRVFEYIPRGVYSDILPSVYIPAIENGEKIA
                     AHVSDANWFELSTIPRYLDISLAMMKNNNVIQGDNCRISPAANIFNSVIWDDVSIGEG
                     ATLNRAIIADGVRIKNGETFENVAIVRAEMLTHCEAIPEKAMKGYHQGENYIVPLKQ"
     gene            2262..3005
                     /locus_tag="VGB68_17855"
     CDS             2262..3005
                     /locus_tag="VGB68_17855"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEY0051164.1"
                     /translation="MSDSLNAKTYAIFSKPSNRKIISLLREAQNEVIEFPLVEAEKIE
                     LNSTQEAFIKNIFEFEWIIFPDAPAVDFFLEALAQANLEFFELDSLRILAFGEAVADR
                     LRFVQIHADVIPARIDAESVFAALRDYLFDEAEFKNLRVLLPKEAAFDTALTGMLRRK
                     ESVVTELNIYRLKPENAAGLAKLKALLKGGAVDEFVFTSPPDVSNLAAVFGEKLKDLL
                     NGTSVSATDETTFQTLREYELRPLYFKKN"
     gene            complement(3054..>3658)
                     /locus_tag="VGB68_17860"
     CDS             complement(3054..>3658)
                     /locus_tag="VGB68_17860"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEY0051165.1"
                     /translation="GDDIDDYAVYRGGASAGQQSFWYYRGSATANGGISVIQFGQNGD
                     FPAPGDYDGDGKYDVCVQRNNGSGQGVFLLRQSSNSTFRSVLWGTSSDLILPGDYDGD
                     GKDDFAVARGSGGQIVWSVLSSNGTSIIHYGQPWGLSATDFPVQGDYDADGKIDIAVW
                     RPNADVQQNFFYVRQSSNGALLTREWGQQGDYPVANYNSH"
BASE COUNT          869 a          932 c          922 g          935 t
ORIGIN      
        1 tatcggcgac caagttataa aaataaatct tgaagtataa atttgcagcc tttggggcag
       61 ataatttttc caggaaagct tcaccgctcg ggaatcaaaa ttcgcaaagc tttctatccg
      121 aatttaattt aactcgtcgc cgataattcg ccgcaaattc ggaaatcttc ccagccgctc
      181 gcaagcctgc caaacgattt gcagcatcgg ctcgataaac gaaatgaagt gagctttctc
      241 gcgaaaaacc gattgatagg aaaacgttcc ggtcgctttc agacagcgct gtatcgtctg
      301 caaatgaaat tctccggcga aaacgtccgg gtcgattgtt tcgagcccga gcttttccct
      361 ttcgctcaaa aagaaaagcc ttttttcgcg cacccacgcg cgcgagggcg gcgctgaaac
      421 tctatccaga agaagcgaga ccaaatcata ggtcaccgag ccgagccgcg cgtcctgatg
      481 gtcgataatc cgcaggcggt tttgacggtc aaacatcaga ttcgccgtgt gaaaatcgcg
      541 gtgacacaag acgctcgcct ttgtttccag ctcgcgcgcg atttccgtaa attcctcgct
      601 caaccggcgt tcgtctgcgc cggaaagctt ttgttttttc aggctttcaa aataatgagt
      661 tttaaaaaaa ttcaattccc agccgagctt ttcctcgtca aatttcagct ttgccgcaat
      721 cgagttcagc tcaaacgctt tcggcgtcgc ggcttgaatc cgcgcgatca ggcgaatcgc
      781 ttcgtttaaa aaatcctcgc gcgcttcatc gttcgttttt tttaaaacgt cgcgcaaaat
      841 ccggtcgccg aaatcttcga gaatgattat tcccgaagct tcgtcaaaag cgaaaatctc
      901 ggcgaccggc agacggcttt ccagaaaaag ctcggtcacg tccaggtaat tatgctcgac
      961 ggcgacaaaa gattcgccgt aaatacaggc gattgccgtt tggttatgcc agtcgatgcg
     1021 aaaaaactcg cgcgtcgaag catccggcgt gagcacttca agtcgaatgt tctcggcgcc
     1081 ggtcttttga tttgaaagaa aatttaataa acgctcgcgg gcggttgttt gaaacataaa
     1141 ttcgtcggcg ggaaaaatcc ggctcactgt ttaagcggaa cgatataatt ttcgccctga
     1201 tgatagcctt tcatcgcttt ttccggaatt gcctcgcagt gcgtcagcat ttcggcgcgg
     1261 acaatcgcga cgttttcaaa agtttcgccg ttttttatac gcacgccgtc ggcgatgatg
     1321 gcgcgattta aagtcgcgcc ttcgccgatg gaaacgtcgt cccagataac cgaattgaaa
     1381 atattcgcgg cgggcgaaat ccggcaatta tcgccttgaa tgacgttgtt gtttttcatc
     1441 atcgccaggc tgatgtccag atagcgcggg atggtcgaaa gctcgaacca attagcgtcg
     1501 gacacgtgcg ccgcgatttt ttcgccgttt tcgattgccg gaatatagac ggacggcaga
     1561 atgtccgaat agacgccgcg cggaatgtat tcgaaaacgc gcggctccag aatatggatt
     1621 ccggtaaaca tcagcggatg ttcgatgttc gacgttcgat tttcgacttc cgctccggtc
     1681 gacggctcgg gaaaattgcc gaaacttttt atcaagccgt tttcgattat aacttccgta
     1741 aatctttcgc gccgcgtgtt cggttttaaa accatcgtcg ccagcgcgcc cgatttccgg
     1801 tgcgtctcga cggcggcggc gatgtcgatg tcggtaataa tttttccgtt gatgattaaa
     1861 aacgtctcgt cttcgagcag atgccgcgcg ttgtcgagcg cgccgctggt tccgagaatt
     1921 tccggcgctt cgattgtgta atggatattg acgccgaaac gactgccgtc gccgagcgcg
     1981 tttttgaccg attccggctg gtgatgaaga ttgacgacca catctcgaaa gccgtatcgg
     2041 gcgacgtatt cggcgacata gccgaccagc ggcttgccga gaaacggtat cgccggtttg
     2101 gtgcggtcga tggttaaagg aaaaagccgc gttccgaaac cggcggcgag aatcatcgct
     2161 ttcatagcat tatttacgat aggtcaattt ttaaaattac ttttttctcg gttcaaatgc
     2221 aaatttgcag attttccatt gtcggctaaa atcgaaaatc tatgtcggat tctttaaatg
     2281 cgaaaaccta cgcgattttt tccaaaccgt cgaaccggaa aattatttcc ctgcttcgcg
     2341 aggcgcaaaa tgaagtgatc gagtttcctt tggtcgaagc cgaaaaaatc gaattaaatt
     2401 cgacgcagga agcttttata aaaaacattt tcgaatttga atggataata tttcccgatg
     2461 ccccggcggt cgattttttt ctggaagcgc tggcgcaggc gaacctggaa tttttcgagc
     2521 ttgattcatt gcggattctg gctttcggcg aagccgtcgc cgaccggctt cgttttgtgc
     2581 aaattcacgc cgatgtgatt cccgcccgga ttgacgctga aagcgttttt gcggctctgc
     2641 gcgattacct tttcgacgaa gcggagttta aaaatctgcg cgttttgctg ccgaaagaag
     2701 ccgccttcga tacggcgttg accgggatgc tgcgccgaaa agaatctgtc gtgaccgagc
     2761 tgaatattta ccgtttaaaa cccgaaaacg ccgccggatt agctaaactg aaagcgcttt
     2821 taaaaggcgg agccgttgac gagtttgttt tcacgtcgcc gccggacgtg tcgaatttag
     2881 ccgccgtttt cggcgaaaaa ttaaaagatt tattaaacgg cacgagcgtt tcggcaacgg
     2941 acgagacgac ctttcaaacg ctccgcgaat acgagttaag accgctttat ttcaaaaaaa
     3001 attgaggctg agaaatttct cagcctcaat tcttaagcaa ctaagcttgt gttttagtgg
     3061 ctgttgtagt ttgccaccgg gtaatcgccc tgctgtcccc attcgcgggt cagtaaagcg
     3121 ccgtttgatg attgtctgac gtagaagaag ttctgctgaa cgtctgcatt cggtctccag
     3181 acggcaatgt cgattttgcc gtcggcatca taatcgccct gaaccgggaa atccgtcgcc
     3241 gacaagcccc acggctgccc gtagtgaatg atgctggtgc cgttcgatga cagaacgctc
     3301 cagacgattt gtccgccgct gccgcgtgcc accgcaaagt cgtctttgcc gtcgccgtcg
     3361 tagtcgcccg gaagaatcaa atcgctcgaa gtgccccaca gaaccgagcg gaaagtgctg
     3421 ttgctcgact gtctgagcaa gaagacgccc tgaccggaac cgttgttgcg ctggacgcaa
     3481 acgtcgtatt tgccgtcgcc gtcataatcg cccggagccg ggaaatcgcc gttttgtccg
     3541 aattggataa ccgagattcc gccgttcgcc gtcgcgctgc cgcgataata ccagaagctt
     3601 tgctgtccgg ctgacgcgcc gccgcgataa accgcgtaat cgtcgatgtc gtcgccgt
//
LOCUS       DASWNE010000913         2684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_101389, whole genome shotgun sequence.
ACCESSION   DASWNE010000913 DASWNE010000000
VERSION     DASWNE010000913.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101389"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1310
                     /locus_tag="VGB68_17865"
     CDS             <1..1310
                     /locus_tag="VGB68_17865"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY0051166.1"
                     /translation="LLGSILSGVSASMEQLIIFRAIQGLGAGALVPLGMTIIGDIFTI
                     TERARMQALFSGVWGFSSVVGPIAGGFITDQLSWRWVFYINLPVGLAAAAIIGIALKE
                     PKVHTRPAIDYAGAGLLMAAITLLMLGLVEGGTGFAALVSPRNLTFFAGFAVLMAIFV
                     WVERRAADPKIPFRLFRNRTVAVSVYAGFLAGVAMFGAISFVPLFAQGALGSTATEAG
                     SLLTPLMLSWVGMSVVGGHLLLRVGYRSISIVGFVFLTVGFVFRSLFERETARFWLYV
                     DLVLIGAGLGLTMLTLLIAVQHAVKRTELGVATSLNQFSRSIGGAVGVAIMGAVLSAG
                     LTAELSYAAQSENAAITVEQAAEFAANPNALIEPQAQAGVSPATLDVLRSAMASAIHR
                     VFWVGTVLAALALLVSLFLPSAGKENNPAEADSISEMDSIKVS"
     gene            1827..>2684
                     /locus_tag="VGB68_17870"
     CDS             1827..>2684
                     /locus_tag="VGB68_17870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051167.1"
                     /translation="MFLCVQLLEVHSQKNSAETPDFRAILQKKLDDFQKIDTKSGQQI
                     KTDGVKLAEICSVDTDAVSRRVFADYGAIFVAEDVEFPQKCIFQNEREVLTYQNAASP
                     KTETIDGTSITLQEEAMEDFLKARRAATKRSLKITPRGGSIAAKRSYTDTRRLWDSRF
                     FPGLKHWVGRRKISPQEAERVKTLPTQQQVAKVLEWESKGWFFSTDFSKSILYSVAAP
                     GASQHLFMLALDVEQFANPAVREILAQHGWFQTVQSDLPHFTYLGLKETELPSKGLKA
                     VFIRGHKFWI"
BASE COUNT          546 a          687 c          767 g          684 t
ORIGIN      
        1 ttctgctcgg ctcgatattg tcgggcgttt ccgcttcgat ggagcagttg attatctttc
       61 gcgccattca agggctcggc gcgggcgcgc tggttcctct gggaatgacg attatcggcg
      121 atattttcac tattacggag cgggcgcgga tgcaggcgct ctttagcggc gtctggggat
      181 tttccagcgt cgtcggtccg attgccggcg gttttatcac cgaccagctt tcgtggcgct
      241 gggtttttta catcaatctg ccggtcggtc tggcggcagc ggcgattatc gggatagctt
      301 tgaaagaacc gaaagttcat acgcgtccgg cgattgatta cgcgggcgcg gggcttctga
      361 tggcggcgat tactttgctg atgctcggct tggtcgaggg cggaacgggt tttgcggctc
      421 tggtttcgcc gcgcaatctc acgttttttg cgggctttgc cgttttgatg gcgattttcg
      481 tctgggtcga gcgccgcgcc gccgacccga aaattccgtt ccggcttttt cgcaatcgaa
      541 cggtcgccgt ttcggtctac gccggatttc tggctggcgt ggcgatgttc ggcgcgattt
      601 cctttgtgcc gcttttcgcg cagggcgcgc tcggctcgac cgcgaccgaa gccggctcgt
      661 tattaacgcc gctgatgctc agctgggtcg gaatgtcggt cgtcggcggg catctgcttt
      721 tgcgcgtcgg ctatcgctcg attagcattg tcggcttcgt tttcctgacc gtcgggtttg
      781 ttttccggtc gctgttcgag cgcgaaacgg cgcgcttctg gctgtatgtc gatttggttt
      841 taatcggtgc gggcttaggt ttgacgatgc tgacgctgct gattgccgtt cagcacgcgg
      901 tcaagcggac ggagctcggc gtcgccacct cgctcaatca gttttcgcgc tctatcggcg
      961 gcgcggtcgg cgtggcgata atgggcgcgg tgctttccgc cgggctgacg gcagagctga
     1021 gttacgcggc gcaaagcgaa aatgccgcca tcacggtcga gcaagccgcc gaatttgccg
     1081 ccaatccgaa cgccttgatt gagccgcagg cgcaggcagg cgtctcgccc gcgacgctcg
     1141 acgttctgcg ctcggcgatg gcaagcgcga ttcaccgcgt tttctgggtc gggacggtgc
     1201 tcgccgcgct ggcgctgctg gtttcgctgt ttctgccgag cgcgggcaaa gaaaataacc
     1261 cggctgaagc ggattcaata tccgagatgg attcaataaa agtatcctga aagtaaagcg
     1321 cgagcggaaa cgataaaaga caggaattcc gccaaccgtt cacataattt cgtctttgcc
     1381 gccggtccgg cgaccggcgc agcgtctgcg atataagcgc cgattttaag acggtctgtc
     1441 ggtaaatttc gcttttaaat ccggcagcgc gtatcttcgg tttctccgtc tatcgacgag
     1501 cgaaatcggt ataaataaga aaagcctcgc gcccggcggg cacaaatata tttttttcgg
     1561 attaaatttc taccggcgct cgcggtcact cattttccaa ctcaaatcca aaactcttta
     1621 ttttaacctt tcgcaatttc agtcagcttc cgacgtctgg tttccggcgc aaagttcgat
     1681 taatttcaaa taatgggcgc gcgtcaaaag gtaatttgca aatgctgaaa aatctctggc
     1741 tttttgcaga aaaatactta taatagctgg tgtcgaatcc ctggacgatg aaatcacata
     1801 aaattagaat attttcgctc ttgttgttgt tcctttgtgt tcaattactg gaagttcatt
     1861 cccagaaaaa ctcggctgag acgccggatt ttcgcgcgat tctccaaaaa aagctcgatg
     1921 actttcaaaa gattgataca aaaagcgggc agcaaataaa gacggacggc gttaaactgg
     1981 ctgaaatttg ttcggttgac acggacgccg tttctcgccg cgtgtttgcc gattacggcg
     2041 caattttcgt tgccgaggac gtggaatttc cccaaaaatg tatttttcaa aatgaacggg
     2101 aagttttaac ctatcaaaac gccgcttctc cgaagacgga aacgattgac ggcacatcga
     2161 taacgcttca ggaagaagcg atggaagatt ttctcaaggc gcgccgcgcg gcgacgaagc
     2221 gcagtcttaa aatcacgccg cgcggcggct caatcgccgc caagcgttcg tacaccgaca
     2281 cccggagact ctgggattcg cggttttttc cgggacttaa gcactgggtc ggacgaagaa
     2341 aaatttcgcc gcaggaagcc gaacgggtaa agaccctgcc gacccagcag caggtggcga
     2401 aagttctgga atgggaaagc aaaggctggt ttttcagcac ggatttttcg aaatcgattc
     2461 tgtattcggt cgccgcgccc ggcgcttcgc agcatctttt tatgctggcg ctggacgtcg
     2521 agcaatttgc caatcccgcg gtgcgcgaaa ttttagcgca gcacggctgg tttcaaacgg
     2581 tgcagagcga tttgccgcat tttacttatc tcggtctaaa agaaacggag cttccgtcaa
     2641 aaggattaaa ggcggttttt atccgcgggc ataagttctg gata
//
LOCUS       DASWNE010000914         2746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_626076, whole genome shotgun sequence.
ACCESSION   DASWNE010000914 DASWNE010000000
VERSION     DASWNE010000914.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626076"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..>2746
                     /locus_tag="VGB68_17875"
     CDS             117..>2746
                     /locus_tag="VGB68_17875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051168.1"
                     /translation="MFSIFSFSKALCFGFILSLFPAAICLGQVPGEIDQTFYSGRTTT
                     KFGVSWNVFKATAIQPDGKIVAAGYHETGSRPNIAVARFNPDGTLDKSFSGDGKLSLA
                     LGDTQADANAVTIQYDGKIILAGFTTTGTKRDFMVARLNPDGSFDNTFSGDGIASTPV
                     SNSNSEIYSMTLQHDGKIAVAGKTGILPNCDFALARFNPNGTLDATFGNGGTLTMPFG
                     TGGDYITSITFRNNKLIVAGYTVNASFNKEIAIARFNLNGSLDNTFSQDGKATLAYLN
                     TDAVAFQVTTQNDGKLVIAGSVHLAGKDNFLLLRYNTDGTLDNTFEADGVVITSLGTG
                     DDAARSVSVQWNGKISVAGYTTTPDGEKHFAAARYNTDGSFDTTFDADGKSSFPIGGF
                     AYSYAMSLQADGKIVLVGASHHPTYQDAVVARLMTDGALDRTFGNRVFTNFGDDDDRA
                     NKVAVQSDGKILVAGWSSNGTNKDFAISRYNASGTPDFTFGNKGLVTTALGSGNDEAR
                     AIVVLPSGKILVAGYSALNNLNHFALARYNANGTLDATFDGDGKAVTAVGASHSIANA
                     MAVQADGKIVLAGIASESGNYDFAVVRYNQDGALDGSFDTDGKLITAISGLGDTARAV
                     VIQADGKIVVAGDGYNGGSNRDFALVRYNADGSIDTAFNNTGKSFVEMGSLNDVPNAL
                     AIQADGKIVVAGQGTFPDLNFVVARYTTDGFLDTDFDIDGRVMTDFIVGNGDSARALA
                     IQADGKIVVGGITTQASATSAAFARYNTDGSPDGEFDASAANPNGDGKTVIPIGESSD
                     FITDIAIQNNGRIVAVGYGQNGTDDDFQVIRLNGTQPALNSAARIPVGGRVLTANGAG
                     IRNVAVTITLPGGETRTTLSG"
BASE COUNT          669 a          740 c          748 g          589 t
ORIGIN      
        1 cgcgaaaaaa caaaaactta ctatatcttt atacggtggc tttcctttcc atctcgttta
       61 tcttcttcat tgttcgtgcc ggttggcaca aaaataaaga aggagaaaac aaaactgtgt
      121 ttagtatatt ttcattttca aaggcattat gtttcggctt tatcttaagt ttatttccgg
      181 cggcgatttg tctcggacaa gttccgggcg agattgacca aacattctac agcggcagaa
      241 cgacgacgaa gttcggcgtt tcctggaacg ttttcaaagc cacggcaatt cagccggacg
      301 gaaaaatcgt ggcggcgggc tatcacgaga ccggcagccg cccgaacatc gccgtcgcgc
      361 gtttcaaccc ggacggcacg ctggacaaaa gtttcagcgg cgacggcaag ctttcgctgg
      421 cgctcggcga tacgcaggct gacgcaaacg ccgtcacgat tcagtatgac ggcaagatta
      481 ttttagccgg attcactacc accggaacca aaagagactt tatggtggcg cgcctgaatc
      541 cggacgggtc atttgacaat actttcagcg gcgacggaat cgccagcact ccggtcagca
      601 acagcaacag cgaaatttat tctatgacgc tgcagcacga cgggaaaatc gctgtcgcgg
      661 gcaagaccgg aatacttccg aattgcgact ttgccctggc gcgtttcaat ccaaacggaa
      721 cgctcgacgc aaccttcggc aacggcggca ctttgacgat gcctttcggc acgggcggcg
      781 attatattac gtccataacg tttagaaaca acaaattaat tgtcgctggc tatacggtca
      841 acgcttcttt taataaagaa atcgccatcg cgcgctttaa tttgaacggc tcgctcgaca
      901 atacttttag ccaggacggt aaagctactc tggcgtattt aaataccgat gccgtcgctt
      961 ttcaggtgac gacgcagaat gacgggaaac tcgtcatcgc cggttcagtc catctggcgg
     1021 gaaaggacaa ttttctactg ctgcgctata acactgacgg cacgctggac aacaccttcg
     1081 aagccgacgg agtcgtaatc acttcgctcg gcacgggcga cgatgccgcc cgctcggttt
     1141 cagtccagtg gaacggtaaa atatccgtcg cgggctacac gacgactccc gacggcgaga
     1201 aacattttgc ggcggctcgc tacaatacgg acggctcatt cgacaccact ttcgacgcgg
     1261 acggaaaatc gtcgtttccc atcggcgggt ttgcttattc ctacgcgatg tcgctgcaag
     1321 ccgacggtaa gatagttctc gtcggcgcga gtcaccatcc gacgtaccag gacgccgtcg
     1381 tcgcgcgttt gatgaccgac ggcgcgctcg accgcacttt cggcaatcgg gttttcacaa
     1441 acttcggcga cgatgacgac cgggctaaca aggtcgccgt ccaatccgat gggaaaattc
     1501 tggtcgccgg ttggagcagt aacggcacaa ataaagattt tgcgatttcg cgttataacg
     1561 cctcgggaac gccggatttt actttcggca ataaaggtct ggtgacgacc gcgctcggaa
     1621 gcggaaatga cgaggctcgg gcgattgtcg ttttgccgag cggaaagatt ctcgttgccg
     1681 gatacagcgc tcttaataac ttaaatcatt tcgctctggc tcgttacaac gcaaacggaa
     1741 cgctcgacgc gaccttcgac ggcgacggaa aagccgttac cgcggtcgga gcttcacaca
     1801 gcatcgcaaa tgctatggct gttcaggcgg acggaaaaat cgttctcgcc ggaattgcct
     1861 ccgaatccgg aaactacgat ttcgcggtcg ttcgttataa tcaggacgga gcgctcgacg
     1921 gcagtttcga cacggacgga aaattaataa cggcgattag cggactcgga gacaccgcca
     1981 gagcggtcgt catccaagcc gacggaaaaa tagttgtcgc cggcgacggc tacaacggcg
     2041 gctcgaaccg cgatttcgcg ctggttcgat acaacgccga cggttcgatt gataccgcct
     2101 tcaataatac cggcaaatca tttgtcgaaa tgggaagctt aaacgatgtt cccaacgcgc
     2161 tggcgattca ggcggacggg aaaatcgtcg tcgccgggca aggaactttt cccgatttga
     2221 atttcgtcgt cgcgcgttac acgacggacg gttttctgga cacggatttt gatattgacg
     2281 gcagagttat gaccgacttt atcgtgggaa atggcgattc cgcccgcgcg ctggcgattc
     2341 aggctgacgg gaaaatcgtc gtcggaggaa taacgacaca agcctcggcg acaagcgcgg
     2401 catttgcccg ctacaacacg gacggctcgc cggacggcga attcgatgct tcggcggcga
     2461 atccgaacgg cgacggtaaa actgtgattc cgattggcga gagcagcgat tttatcaccg
     2521 acattgccat tcaaaacaac ggcagaatcg tcgccgtcgg ctacgggcaa aacggaacgg
     2581 acgatgattt ccaggtaata cgtctgaacg gcacacaacc ggctctaaac tctgccgcgc
     2641 ggattccggt cggcggacgc gttctgaccg caaacggcgc cggaatcagg aatgtcgcgg
     2701 taaccatcac attgccgggc ggcgaaacgc ggacgacgct ttcagg
//
LOCUS       DASWNE010000915         6298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_595760, whole genome shotgun sequence.
ACCESSION   DASWNE010000915 DASWNE010000000
VERSION     DASWNE010000915.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6298
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595760"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VGB68_17880"
     CDS             <1..309
                     /locus_tag="VGB68_17880"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEY0051169.1"
                     /translation="TQHVERLIKAGKMKAAGMKEVEAAKRDGRWQRAYESPKNMSVPE
                     DFLKELEKDAKAGAFFETLDKTNLYAIAFRLQTAKKPETREKRMRAILEMLSKGEKFH
                     "
     gene            complement(438..2888)
                     /locus_tag="VGB68_17885"
     CDS             complement(438..2888)
                     /locus_tag="VGB68_17885"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY0051170.1"
                     /translation="MKNIKVAFFVVVFVLANSTFSFAQSFYQKPPKEITDVLGAPASP
                     LTSTSPAKDKIALLEPLRYPPISELAQPMLRLAGLRINPNTTGQHRQPYYVKVTLKNV
                     ADGRETPVALPDGAQIISPSWSADGRYLAAGNVTPTGIDLWIIETATARARMVKNLKV
                     NTAFGSWQWMPDQKTLLVNAVAKNRGAAPGYQNLTPTGPNIQEGAGRGGAVQTFQDLL
                     RSPVDEALFDYYASSQLVAVDASGAKTIAEIGQPAIFETVSPSPDGKNILVARIRKPY
                     SYLYPYFRFPKEVEVWDAQGNRVYKLASVPLQDKIPVQGVQVEPRGYGWVPTEAATLM
                     WAEALDNGDPRTSITPRDKLVRLAAPFSAQPEEVLRTEQRYSGRTFGETAGTMFVYDY
                     NRDTQRRRIFMLDYRAKSAPRLISDLNINDRYNDIGNFVTKRLPNGYSVVQQNGDYVF
                     LTAEGSTPEGDRPFLRRFNLQTLKAEDIFRSRNDDYESFVAFADDKGMNFFTRKESIA
                     APPNLFINYRCEPKEVCTAPVPKQITNFTDTTPQLRGIKKQLVKYKRADGVDLSFTLY
                     LPPNYEEGTRLPTIVWAYPLEFTDASTAGQVSGSTNRFTQLGGTSHLFFLLQGYAVLD
                     EATMPVVGNPETVNDTFIRQIVDSARAAIDKGVEMGVVDPERVGVGGHSYGAFMTANL
                     LAHSDLFRAGIARSGAYNRTLTPFGFQGERRTFWEATDLYTKISPFFFAHKINEPILL
                     IHGEADNNQGTFPIQSERLYAAIRGNGGTSRLVMLPLESHGYSARESTEHTLWEMLSW
                     FDKYVKNAPPRKQTATKE"
     gene            complement(2941..3291)
                     /locus_tag="VGB68_17890"
     CDS             complement(2941..3291)
                     /locus_tag="VGB68_17890"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY0051171.1"
                     /translation="MKRFLLLNLAFSLMLAGGAISTNAKPKKIKTVRINISQQGYAPA
                     SVAVKKGETVRLVFYRADANNCGEELVFPALNIKRQIPVRRRVAVTLTPRRTGEISFA
                     CGMDMYRGKILVTN"
     gene            complement(3358..4722)
                     /locus_tag="VGB68_17895"
     CDS             complement(3358..4722)
                     /locus_tag="VGB68_17895"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEY0051172.1"
                     /translation="MKNFTAFIKYSALVSLSLLLFACADSPKQSTAGESKNESKNKAS
                     NSQAILVSAPDADSAEPAIAAAPEGGVYLIWVEHGAGRAADLYFQKFDAEAKAVGEKV
                     RVNPRAGEATAWRGDPPTIVVGANGEIFIGWTASVKTEKASGTNFYVSVSRDGGKSFG
                     APAKVNDDSEPASHGMHSLAADESGKIHAAWLDERNIKAPAHAGNKPEISNDFQYVKA
                     HHTPTPEVKKAQETEAAEPNSEIFYAVSKDGAKTFSTNKKISSEVCPCCKTSLLTNGD
                     GKAYVSWRQVLEGDFRHIAVASTIDGGESFSAPAIVSDDRWQIAACPVSGASMTLAET
                     GALKVAWFTAGAAGKPGLYTAESTDGGKSFSARTLINENATSGNPLLFAREKGGFAVV
                     WEAGGKLFRAGIEANQIGRAEEIADGTVPSAALSKGKIFIGFSRKETDKRGVWLAVFD
                     DLQT"
     gene            complement(4727..5728)
                     /locus_tag="VGB68_17900"
     CDS             complement(4727..5728)
                     /locus_tag="VGB68_17900"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY0051173.1"
                     /translation="MKKISALLILPLIVCLFFVSARAQKKTVPKKSAPTNVQKYEPPV
                     IENAPELQSVLSKAVGETLNKFISKKMQPDNLAVTLIDLRDPKNLKMANFRGDAKIYP
                     ASVVKMFYLVAVHEWLKNGKLKDTPELRRALKDMIVDSSNDATHQIVDVLTGATGGGE
                     LSPPELKIWSNKREAVNRYFKSKNYADINVVQKTYCEDLYGRERQFWNGGKYRNMLTT
                     NSTARLLAEIALGKAVSTQASVEMLELLKRNPYKQDKDLDNQDIGFTGIALKNIRNAN
                     LWSKAGWTSNSRHDAAYVETPDGQKFVLVVFTEKFANEREFIPDIAAKILQELRGKN"
     gene            5917..>6298
                     /locus_tag="VGB68_17905"
     CDS             5917..>6298
                     /locus_tag="VGB68_17905"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY0051174.1"
                     /translation="MTEEKLVRGISRWDLTAIAINTIIGTGIFLLPARVTGLIGSYSL
                     FAFVVCAVIVAFIVLCFAEVSSRFQATGGMYLYAREAFGSVVGFEVGWLYWIVRITTF
                     ATNCNALLLYIEFFNPSLAQGFWRV"
BASE COUNT         1340 a         1661 c         1743 g         1554 t
ORIGIN      
        1 acgcagcacg tcgagcgctt aatcaaagcg ggaaaaatga aagccgccgg aatgaaggaa
       61 gtcgaagcgg caaagcgcga cggtcgctgg cagcgcgctt atgaatcgcc caaaaatatg
      121 tcggttcccg aagatttttt aaaggaattg gagaaagacg ccaaagccgg agcttttttt
      181 gaaacgctcg acaaaacaaa tctctacgcc atcgcctttc gtctgcaaac ggcaaagaaa
      241 ccggaaacgc gcgaaaaacg aatgagagcg attctggaaa tgttgtcgaa aggcgagaaa
      301 tttcattgat tgcccggatt gacggtcgtt tagcgtcgag cggcggaagc atttatgcgg
      361 aagatattag taaaaaggcg aaaggcaaaa gtagcttgat tttcactttt gcctttcgcc
      421 ttcgcctttt tattttctta ttcttttgtc gcggtctgct tgcgcggcgg agcgtttttg
      481 acgtatttgt cgaaccagct gagcatttcc cagagcgtgt gctcggtcga ttctcgcgcc
      541 gaatagccgt gcgattcgag cggcagcata accaggcggc tcgttccgcc gttgccgcga
      601 atcgccgcgt agagccgctc ggattgaatc gggaaagttc cctgattgtt gtcggcttcg
      661 ccgtgaatca gcaaaatcgg ctcgttgatt ttgtgtgcga aaaagaacgg cgaaattttc
      721 gtgtaaaggt cggtcgcttc ccagaaagtg cggcgctcgc cctgaaagcc gaaaggcgtc
      781 agggttcgat tatacgcgcc cgagcgcgct atcccggcgc gaaacaaatc cgaatgagcc
      841 agcagattag ccgtcataaa cgcgccgtaa gagtgtccgc cgacgccgac tctttccggg
      901 tcgacgacgc ccatctcgac gcctttatca atcgccgctc gcgccgaatc gacgatttgc
      961 cggataaacg tgtcgttgac ggtttcggga ttgccgacga ccggcatcgt cgcctcgtcc
     1021 agcaccgcgt agccctgaag cagaaagaaa agatgcgagg ttccgccgag ctgcgtaaaa
     1081 cgattggtcg agccggaaac ctgtccggcg gtcgaagcgt cggtaaattc gagcggatac
     1141 gcccagacga tggtcggcag gcgcgtgcct tcctcgtaat tcggtggcag gtagagcgta
     1201 aacgacaaat cgacgccgtc ggcgcgtttg tatttcacca gctgtttttt gatgccgcgc
     1261 agctgcggcg tcgtgtcggt gaaattggta atctgtttcg gcacgggcgc ggtgcagact
     1321 tccttcggct cgcagcgata attgataaac agattcggcg gagccgcgat tgattccttg
     1381 cgcgtaaaga aattcattcc tttgtcgtcg gcaaaagcga cgaagctttc gtaatcgtca
     1441 ttgcgagagc gaaaaatatc ttccgccttt aaagtctgca ggttaaaacg acgcaggaac
     1501 gggcggtcgc cttcgggcgt cgagccttcc gccgtcagga aaacgtaatc gccgttttgc
     1561 tggacgacgc tgtaaccgtt cggcaggcgt ttggtgacaa aatttccgat gtcgttgtag
     1621 cggtcgttga tgtttaaatc ggagatgagc ctgggcgcgg atttcgcgcg gtaatcgagc
     1681 ataaaaattc gccgccgctg cgtgtcgcgg ttatagtcgt aaacgaacat cgtcccggca
     1741 gtttcgccga aagtcctgcc cgaatagcgc tgctcggtgc gcagcacttc ttcgggctgc
     1801 gcgctaaacg gcgcggcgag ccgcacaagc ttgtcgcgcg gcgtaatgct cgtgcgcggg
     1861 tcgccgttgt cgagcgcttc cgcccacatc aaagtcgccg cttcggtcgg aacccagccg
     1921 tagccgcgcg gctcgacctg cacgccctgc acgggaattt tgtcctgcaa aggaacgctc
     1981 gccagcttgt agacgcggtt gccctgcgcg tcccagacct cgacttcttt cggaaatcgg
     2041 aaatacgggt aaagatacga ataaggcttc cggatgcgcg ccacaaggat atttttaccg
     2101 tcgggcgatg gcgaaacggt ttcaaaaatc gcgggctgac cgatttcggc gattgttttc
     2161 gcgccgctcg cgtcgaccgc gaccagctgc gaagacgcgt agtaatcaaa caaggcttcg
     2221 tcaaccggcg agcgcagcaa atcctgaaaa gtctgcaccg cgccgccgcg tcccgcgcct
     2281 tcctgaatat tcggtccggt cggcgtcagg ttttgataac cgggagccgc cccgcgattt
     2341 ttcgcgacgg cgttgacgag cagggttttc tggtcgggca tccattgcca cgagccgaaa
     2401 gccgtgttca ccttcagatt tttaaccatt ctggctcgcg ccgtcgcggt ttcgataatc
     2461 cagaggtcga tgccggtcgg cgtcacgttt ccggcggcga gatacctgcc gtccgcgctc
     2521 cagctcggcg agataatctg cgcgccgtcg ggcagcgcga ccggagtttc tcgaccgtcg
     2581 gcgacatttt tcagcgtcac ttttacataa tacggctggc ggtgctgtcc ggtcgtattc
     2641 ggattaatcc ggagcccggc gagccgcagc atcggctgcg cgagctccga aatcggcgga
     2701 tagcggagag gttcgagcag cgcgatttta tctttcgccg ggctggttga tgtcaagggc
     2761 gaggcgggcg cgccgagcac gtccgtaatt tccttcggcg gtttctgata gaagctctgg
     2821 gcgaaagaaa acgtcgaatt tgccagaacg aaaacgacta caaaaaatgc gactttaatg
     2881 tttttcattt tttacctttt aaacttggaa tggctttaag aaaattttag cttaaagcgc
     2941 tcaattcgtc acgagaattt ttccgcgata catatccatt ccgcaggcaa acgagatttc
     3001 gcccgttcgc ctcggcgtga gcgtgacggc gacgcgccgc cgcacgggaa tttgacgttt
     3061 tatgttgaga gccggaaaaa ccagttcctc gccgcagtta ttggcgtccg cgcgataaaa
     3121 gacaagcctc acggtttcgc cttttttgac ggcgaccgaa gcgggcgcgt aaccttgctg
     3181 gctgatatta atgcggacgg tttttatttt cttcggcttg gcatttgtcg aaatcgcgcc
     3241 gccagccagc attagcgaaa aagctaaatt gagtaacaaa aatcttttca ttccaatctc
     3301 cttattgaag ataaattaag cgcgcagaga gatgatgatg cgaacaggtt tcgggcttta
     3361 cgtctgtaaa tcgtcgaaaa cagccagcca gacgccgcgt ttatcggttt ctttgcggct
     3421 gaaaccgata aaaattttgc ctttggaaag agcggcggaa ggcactgtgc cgtcggcgat
     3481 ttcttcggct ctgccaatct gattcgcctc gattcccgcg cggaataatt taccgcccgc
     3541 ttcccagacg acggcgaatc cgcctttttc gcgggcgaaa agcagcggat tgccggaggt
     3601 cgcgttttca tttatcaacg ttcgggcgga gaaagatttt ccgccgtccg tcgattcagc
     3661 cgtgtaaagt ccgggctttc cggctgctcc cgccgtgaac caggcgactt tcagcgcgcc
     3721 cgtttcagcc aaagtcatcg acgcgccgga aaccggacaa gccgcaatct gccaccggtc
     3781 gtcgctgaca atcgccggag cggaaaagct ttcgccgccg tcaattgtcg aggcgacggc
     3841 gatatgccgg aaatcgcctt ccagaacctg ccgccagctg acgtaagctt tgccgtcgcc
     3901 gttcgtcaac agcgaagttt tacagcaggg gcagacctcg ctcgatattt ttttattcgt
     3961 cgaaaacgtt ttcgcgccgt ctttggaaac cgcgtaaaaa atctcgctgt tcggctcggc
     4021 tgcttcggtt tcctgcgcct ttttaacttc gggcgtcggc gtgtgatgcg ctttgacgta
     4081 ttgaaaatcg tttgaaatct caggtttgtt tccggcgtga gccggcgctt ttatatttcg
     4141 ctcgtccagc cacgcggcgt ggatttttcc gctttcgtcc gccgccagag aatgcattcc
     4201 gtgcgaggca ggctcggaat cgtcgttgac tttcgcgggc gcgccgaaag attttccgcc
     4261 gtcgcgcgaa acggaaacgt aaaagttcgt gccgctcgcc ttttcggttt tcacgctcgc
     4321 cgtccagccg ataaaaattt cgccgtttgc gccgacgacg attgtcggcg ggtcgccgcg
     4381 ccacgcggtc gcttctccgg ctcgcggatt gactcggact ttttcgccga cggctttcgc
     4441 ttcggcgtcg aatttttgaa aatagaggtc ggcggctctg cccgcgccgt gttcgaccca
     4501 gatcagataa acgccgccct cgggcgcggc ggcgattgcc ggctcggcgg aatcggcatc
     4561 aggcgcggaa acaagaatcg cctgcgaatt tgaagctttg tttttcgatt cgtttttcga
     4621 ttcgccggca gtcgattgct ttggcgaatc ggcgcaggca aaaagcaaaa gcgaaagcga
     4681 aacgagcgcc gaatatttta taaatgctgt aaaatttttc atctaactaa ttttttccgc
     4741 gcagttcctg cagaatcttt gcggcgatgt cgggaataaa ctctcgctcg ttggcgaatt
     4801 tttcggtgaa aacgacgaga acgaatttct gaccgtcggg cgtttcgacg taagcggcgt
     4861 cgtgacgcga gttgctcgtc cagccggctt tcgaccagag atttgcgttg cgaatgtttt
     4921 tcagcgcgat gcccgtaaat cctatatcct gattgtccaa atccttgtcc tgtttatacg
     4981 gattgcgttt cagtaattcg agcatttcta cggacgcttg cgtcgagacg gcttttccca
     5041 gagcgatttc ggcgagcagt cgcgcggtcg aattggtcgt cagcatattg cggtatttcc
     5101 cgccgttcca gaactgccgc tcgcgcccgt agaggtcttc gcaataagtt ttctgcacga
     5161 cgttgatgtc cgcgtaattc ttcgatttga aatagcgatt gacggcttcg cgcttattcg
     5221 accagatttt caattccggc ggcgagagct cgccgccacc ggttgcgccg gtcagaacat
     5281 cgacgatttg atgcgtcgcg tcgttggacg aatcgacaat catatctttc agcgcgcggc
     5341 gcagctcggg cgtgtctttc aattttccat tcttcagcca ttcgtgtacg gcaaccagat
     5401 aaaacatttt gacgacgctc gccggataga ttttcgcgtc gccgcgaaag tttgccattt
     5461 tcaggttttt cgggtcgcgc aagtcgatca gagtgacggc gaggttgtcg ggctgcattt
     5521 ttttcgaaat aaatttattt aaagtttcgc cgaccgcttt cgacaaaacg ctctgcagct
     5581 ccggcgcgtt ttcaatcacc ggcggctcgt atttttgaac attggtcggc gcggattttt
     5641 ttgggacggt tttcttttgc gcccgcgcgg aaacgaaaaa aagacaaacg atcagcggca
     5701 aaattaaaag cgcggaaatt tttttcattc ggaaaactcc tcggaagtct ttgcggaaag
     5761 cgttaaagca gagtataaac gaaaagcgtt tgaaaattcc agattccagg ttcaagattc
     5821 aggattcaag attcaggatt caagattcag gattcaagat tcaagattta agattgaaag
     5881 aaaattccca atcctgaatc ctgaatcctt tcagatatga cagaagaaaa acttgttcgc
     5941 ggcatcagcc gctgggattt gacggcaatc gccatcaata cgattatcgg tacgggaatt
     6001 ttcctgctgc cggcgcgggt gacgggttta atcggcagct acagcttgtt tgcgtttgtc
     6061 gtctgcgccg ttatcgtcgc gtttatcgtt ttatgtttcg ccgaagtctc cagtcgtttt
     6121 caggcgacgg gcggaatgta tctttacgcc agagaagcgt tcggctcggt cgtcggtttc
     6181 gaggtcggct ggctttactg gattgtgcgc atcacgacct ttgccaccaa ctgcaacgcg
     6241 cttttactct acatcgagtt ttttaatccg tccctggcgc aaggcttctg gcgcgttc
//
LOCUS       DASWNE010000916         4067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_341810, whole genome shotgun sequence.
ACCESSION   DASWNE010000916 DASWNE010000000
VERSION     DASWNE010000916.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341810"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VGB68_17910"
     CDS             <1..708
                     /locus_tag="VGB68_17910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051175.1"
                     /translation="ESVDLIMLTGGITDIRLSVILNPLKDNDELRREIARHCNEKMFE
                     MLKKAATEFPNALIVVVGYYPFLSKYTPARTMLNNLLEIYEFPRPLKSAINNPLNRRL
                     LKRYRKKMIERSLIWAHDSTREFKKAVARLNTESGKQRAVFVESPIKEENAIAAVKPL
                     VYETGKKGRAQDALAEKRLKVCDETIDELKKLTDLKFRTRTCELATIGHPNVEGSKAI
                     AEAIKENLKPFFQSAAE"
     gene            complement(718..1098)
                     /locus_tag="VGB68_17915"
     CDS             complement(718..1098)
                     /locus_tag="VGB68_17915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051176.1"
                     /translation="MHDFVLYLQLVVYVLMVLVYLPKLVVQVISFISLLKKKKVERSK
                     EIKSSPAKFSLKISNMLPKKHAQSLLQEISDMRLEYYEALSEKKIWRAKCIVAFYYVG
                     LSWSVVMWISDKFKEVIGIIPKKN"
     gene            complement(1345..1632)
                     /locus_tag="VGB68_17920"
     CDS             complement(1345..1632)
                     /locus_tag="VGB68_17920"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY0051177.1"
                     /translation="MLLSKTEALILQIMSENGFREVYGLELLKLSNGGIKRGTIYTTL
                     QRMEDKGFVTSRQEEKPDDVSGIPRRLYEITGTGQRALQAYELMFGNIVMA"
     gene            1777..2886
                     /gene="mnmA"
                     /locus_tag="VGB68_17925"
     CDS             1777..2886
                     /gene="mnmA"
                     /locus_tag="VGB68_17925"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEY0051178.1"
                     /translation="MKIAVAMSGGVDSSSAAALLKEQGHELVGFTMQLWNQRRNINVD
                     ENGDPLPSRCCSLDDVYDARRVAESLGMPFYVLNLEKDFEESVVEPFVQSYLSGETPI
                     PCVACNSRLKFASLDKLAQSLGCDKVATGHYARVEYDEAANRYRLFRGKNHWKDQSYF
                     LWELTQDQLSRAFFPLGEMLKSEVREIAREANLYVAEKQESQEICFVPDGKYSEFIDR
                     YLEHENRSQELPEAGEIVNAKGEKMGEHSGIHRYTIGQRRGLGIANEKPLYVVQIEQA
                     RNQIVVGEKDELEQLEFTAKGVNWVAFDESSEPVRAEVKVRYRHEPAPATVYALPERR
                     ARIVFDEPQRAITPGQATIFYNGEEVIGGGWIVKI"
     gene            2944..3132
                     /locus_tag="VGB68_17930"
     CDS             2944..3132
                     /locus_tag="VGB68_17930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051179.1"
                     /translation="MAKEKKRLSKSAKAALKTLEESKLLAASKTITDSKADQTFKPSD
                     SVVKPSAPNKLRPEKKRG"
     gene            complement(3272..>4067)
                     /locus_tag="VGB68_17935"
     CDS             complement(3272..>4067)
                     /locus_tag="VGB68_17935"
                     /inference="COORDINATES: protein motif:HMM:NF012762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cellulose binding domain-containing protein"
                     /protein_id="HEY0051180.1"
                     /translation="DDKVALLHRLWNYTPQAPEHGCSDGRDADGDGLLDYPADPGCEN
                     AEDDDEFNAPIGSQGLELTTVTNNDWGTGYCREIRVRNTNNFDIDWLINQTIEGEITS
                     IWSGAYTRNGNEVGFGGTWNDILDAGKETTFGFCVTRPAPPPPACADGQDNDQDGLVD
                     LNDPGCATAQDNDEANAPPSSEGLTTDINIVNDWGGGYCADVTVANTTANPIDWTVSF
                     TIEGTVSGLWNVAYVQNGNTVTAEGFSWNNIIQPQGSLAFNFCANR"
BASE COUNT         1121 a         1003 c          958 g          985 t
ORIGIN      
        1 gaatcggtcg atttgataat gctcaccggc ggcatcacgg acattcgcct ttcggttatt
       61 ctcaaccctt taaaagacaa cgacgagctg cggcgcgaaa tcgccagaca ctgcaacgaa
      121 aagatgttcg aaatgctcaa aaaagccgcg accgaatttc cgaacgcgct gattgtcgtc
      181 gtcggctact acccgtttct gtcaaaatac acgcccgccc gcacaatgct caataatctt
      241 ctggaaatct atgaatttcc gcgcccgctg aaatccgcga ttaacaaccc gctgaatcgc
      301 cgcctgctca aacgctaccg caaaaaaatg attgagcgct cgctgatttg ggcgcacgat
      361 tccacgcgcg aattcaaaaa agccgtcgcg cgtctaaaca ccgagtccgg caaacaacgc
      421 gccgtcttcg tcgaatcgcc gatcaaagaa gaaaacgcca tcgccgccgt aaaacctcta
      481 gtttatgaaa ccggaaaaaa aggtcgagcg caggacgcgc tggctgagaa aagattaaaa
      541 gtctgcgacg aaacgattga cgaactaaag aaactaaccg atttgaaatt ccgcacccga
      601 acctgcgagc tcgccaccat cggacacccg aacgtcgaag gctcgaaagc catcgccgaa
      661 gcgataaaag aaaacctgaa accgtttttt caatctgctg cggaatgaga tagattgtta
      721 atttttcttt ggaataattc cgataacttc cttaaattta tctgaaatcc acatcacaac
      781 agaccaactt aaaccaacgt aataaaaagc aacgatacat tttgctcgcc agattttctt
      841 ttctgataat gcttcgtaat attctagtcg catatcagaa atttcttgaa gcaaactttg
      901 agcgtgtttt ttaggaagca tgttagaaat tttgagtgag aatttagcag gtgaactttt
      961 tatctcttta cttctttcaa ctttcttctt tttaaggagt gaaatgaaag atataacttg
     1021 cacaactaac tttggcagat agactaatac catgaggaca taaacaacaa gctgtaaata
     1081 taaaacgaaa tcatgcatag tcgtaaaatg ttattttgcc ttcacaaaag gttcgtatga
     1141 aattttatca aatttcatat ttaatacaaa cattttttta aacgctaaag ataaatttcg
     1201 aataaattca atcaggtcat taatagaacc gcttgttagc ttatttctta acacaattct
     1261 aaaatagaag tagccaccaa aatcaagcta cctgtcaaaa atgaaacagc taacgataac
     1321 ttcactacat taaactccaa caaactatgc catgacaata ttgccaaaca ttaattcata
     1381 agcttgcaaa gcacgctgtc ctgttcctgt aatttcataa agtcggcgcg ggattcccga
     1441 aacgtcgtcg ggcttttctt cctgtcgtga agttacaaaa cctttgtctt ccattcgttg
     1501 aagcgtcgtg taaatcgttc cgcgtttgat tccgccgttg gagagtttca gaagttccaa
     1561 gccgtagact tcgcggaagc cgttttccga cattatttgt aaaattaaag cctctgtttt
     1621 tgataaaagc atatttttgt cctttaaaat caccggtctt acaaaattta agagcggctt
     1681 ttacaaaaga taagacagtt gtttttgaaa gtcaagcaat ttttgaaaag tttccaacat
     1741 ccaagcaaac cgttatactg tcagtttgga gtgattatga aaatcgcggt ggcaatgagc
     1801 ggcggcgtcg attcttcgag cgcggcggct cttttaaaag aacagggaca cgaattggtc
     1861 ggctttacga tgcagttgtg gaatcagcga cggaatatta acgtcgacga aaacggcgac
     1921 ccgctgccgt cgcgctgctg ctcgctggac gacgtttacg acgcccggcg cgtggcggaa
     1981 agccttggaa tgccgtttta tgttttgaat ctcgaaaagg attttgaaga atcggtcgtc
     2041 gagccttttg tgcagagcta tctttcgggc gagacgccga ttccgtgcgt cgcgtgtaat
     2101 tcgcgtctga aattcgcgtc gctggacaaa ctggcgcaat cgctcggctg cgacaaagtg
     2161 gcgaccgggc attacgcgcg cgtcgaatac gacgaagcgg cgaatcgtta ccggctgttt
     2221 cgcggtaaaa atcactggaa agaccagagc tattttctct gggaattgac gcaggaccaa
     2281 ttgtcgcgcg ccttttttcc gctcggcgaa atgctcaagt ccgaagtgcg cgagattgcg
     2341 cgcgaggcga atttgtatgt cgccgaaaaa caggaatcgc aggaaatctg ttttgtgccg
     2401 gacggcaaat attccgagtt tatcgaccgc tatctggaac acgaaaatcg ctctcaggag
     2461 ctgcccgaag cgggcgaaat cgtcaatgcg aaaggcgaaa aaatgggcga gcattcgggc
     2521 attcatcgct acactatcgg tcagcgccgc gggctcggca tcgcgaatga aaaaccgctt
     2581 tatgtcgtgc agatcgagca agcgcgaaat caaatcgtcg tcggtgaaaa agacgagctc
     2641 gaacaactcg aatttacggc aaaaggcgta aactgggtcg cctttgacga atcctcggag
     2701 ccggttcgcg ccgaagtcaa agtccgttat cgtcacgagc ccgcgccggc gaccgtttac
     2761 gctttgcccg aaaggcgcgc gcgaatcgtt ttcgacgagc cgcagcgcgc tatcacgccc
     2821 ggacaggcga cgattttcta taacggcgaa gaagttatcg gcggcggctg gattgtaaaa
     2881 atctgattta atcggttaat ttataaaaac gacagacttc aaaacggaga aagggaataa
     2941 acaatggcga aagaaaaaaa gagattgagt aaaagcgcaa aagcggcgct aaaaaccctg
     3001 gaagaatcaa aattgctggc ggcttcgaaa acgataaccg actcgaaagc cgaccaaact
     3061 tttaagccgt cggactctgt ggttaaaccg agcgcgccga acaaactgcg accggaaaag
     3121 aaacgaggct gaaaataaac cgccgaccca agtccaataa tcgaaaccgg aataatgcgg
     3181 cgcggacaaa cggcgatttt gcatctcaaa aataatttag aggcgaaact gttcgcctct
     3241 aaattattga ttactgtttt tgcggcagct tttatctgtt agcgcagaaa ttaaacgcca
     3301 gcgagccttg cggctggatg atgttgttcc acgaaaaacc ttccgccgtg acggtatttc
     3361 cgttttggac ataggcgacg ttccagagac cggagacggt tccttcaatc gtaaaactca
     3421 ccgtccagtc aatcgggttc gcggtcgtat tcgccaccgt gacgtcggcg cagtaaccgc
     3481 cgccccaatc gttgacgata ttaatatcgg tcgtcaaacc ttcgctggac ggcggcgcgt
     3541 ttgcctcgtc gttgtcctgc gcggtcgcgc agcccgggtc attcaggtca actaatccgt
     3601 cctggtcgtt gtcctgaccg tcggcgcagg caggcggcgg cggcgcggga cgcgtgacac
     3661 agaaaccgaa cgtcgtttct tttccggcat cgagaatgtc attccacgtt ccgccgaagc
     3721 cgacttcgtt tccgtttcgc gtgtaagctc cggaccagat actcgtaatt tcgccttcaa
     3781 tggtctggtt gataagccag tcgatatcga aattgttcgt gttcctgacg cgaatttccc
     3841 tgcagtagcc cgttccccaa tcattgttgg taacggtcgt caactccaag ccctgactgc
     3901 ctatcggagc attaaactcg tcgtcgtctt cggcgttttc gcagcccggg tctgccggat
     3961 aatccagcaa cccgtcgccg tcggcgtcgc gcccgtcgga gcagccgtgt tccggcgcct
     4021 gcggcgtgta attccataag cggtgcagca gagcgacttt atcgtcc
//
LOCUS       DASWNE010000917         5062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_489350, whole genome shotgun sequence.
ACCESSION   DASWNE010000917 DASWNE010000000
VERSION     DASWNE010000917.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5062
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489350"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2010)
                     /locus_tag="VGB68_17940"
     CDS             complement(<1..2010)
                     /locus_tag="VGB68_17940"
                     /inference="COORDINATES: protein motif:HMM:NF020498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HEY0051181.1"
                     /translation="MRKALFGLAAVIFSIAFFGFYLPAQKAQENPFRNMEIITTLQRS
                     SQKNQTPPSAQIKNIQVFGEKSGMAHTSDALFRTDDDGETWREIILPRNNAEKIAAVY
                     FQNENAGLAILTDSRNARLELAKTLDGGNNWTKTPIELRSEDLQQADLEQVGLLIYES
                     EDFRDLELLLRAPTSSSFTAKIHYETTDGGNNWTFTKRSHSLRRSDESFENISGNWTL
                     KTEGDCEGFKTGCIQETKIFNRAREITPPRIKELARLEKENARLQAQNSVFAAPPNGS
                     TRISLNRGFDKCTAATAAQMQTWWNNSPFYDANIYISGRNRGCAQPQLTAAWVNQVSA
                     MGWGLIPTIVGYQAPCSVCTSCQKHSSDPALAETQGRGEADIAIVDANNLGLTQGAVL
                     YYDMERYDDVSGTGACSTPVKAFLKGWTDRLKEQGYISGVYGSPTNAVGDWINIPAPS
                     RMDAIWMARWDNVASVWFYASPSPMIPNDIWANHQRIKQYQAPHNETWGGVTFNIDGN
                     IADGPVAGVQPAKNKTADFDGDGRTDISVFRPETGSWYLVSSSNSGFSGINFGIANDI
                     LAPGDYDGDGKTDYAVFRPSDGTWYIQLRTTLRAQQFGTNGDIPVPADYNGDGFTDVA
                     VFRPSNGYWYIWNSSGSPNLLRVEQFGANGDKPAPGDYDGDGKTDL"
     gene            2304..2705
                     /locus_tag="VGB68_17945"
     CDS             2304..2705
                     /locus_tag="VGB68_17945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051182.1"
                     /translation="MARTELSSSASRPPVHHRIMANLQVQASWIDKVTGKTVLVEGLT
                     ENIGETSALVNLTTLPPVGSEVNLRIIDEEKTLIEVPTEVIRVERDPSKPLAALSVGE
                     NLKKWKTTVMTAAQEWVTRQWKLNYEEEWVN"
     gene            2868..3944
                     /locus_tag="VGB68_17950"
     CDS             2868..3944
                     /locus_tag="VGB68_17950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051183.1"
                     /translation="MKKFIALALILISAGFIFAQETPPQQKPLSQTEYVRLLYDLEKN
                     PKKRDEVIETVRKRGIGFTVTDGIKGLTVTKSRNDAELKRTLEEASRRRENPAASQPP
                     SEKESAEVLAKSREATLAAVDEMPDFVVKQMIQRSEAYAGTSNFKNRDRLIVAVSYRS
                     EGREEYKILSLNGVLQENPKAKSAYSEVGGTSSTGEFVTVLATIFKAESDTKFEVIDT
                     DLIRGRRAVVYGFDITKDKAKQQITASGIVSSSTLSGMKGKIWIDRESFRVLRVESAA
                     TEIPADFPIRAASRAIDYDWVMISEQKFLLPLMSDVRLTFRENRQLYETRNLIRFKEY
                     QKFGSDVKILDSDDEEVTEEKPNQ"
     gene            complement(3993..4169)
                     /locus_tag="VGB68_17955"
     CDS             complement(3993..4169)
                     /locus_tag="VGB68_17955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051184.1"
                     /translation="MKNSYKKVIFMMMPETPEKRKFPASQWIKRFGFAGFMFFLIKGL
                     LWLIVPALVAYLAW"
     gene            4198..4935
                     /locus_tag="VGB68_17960"
     CDS             4198..4935
                     /locus_tag="VGB68_17960"
                     /inference="COORDINATES: protein motif:HMM:NF013586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEY0051185.1"
                     /translation="MKKHFRRFTAPLFCFILFSSAVFAQSAAAPREENKREADLVELV
                     KLDKTIKLDIRYARNDNFVGKIVYTEARAFLQRPAAEALVRVHKQLKKQNLGLIIFDG
                     YRPWSVTKIFWEVTPEDKRKFVANPTTGSKHNRGCAVDLSIYDRKTGKLIEMPSDFDE
                     FTERASPDYQGGTEKERANRDLLRRLMEAEGFTVNANEWWHFDYKDWQDYAIYDIPFS
                     AIGSSRAKSSKANLKKNQPRKTKTSNP"
BASE COUNT         1321 a         1231 c         1256 g         1254 t
ORIGIN      
        1 caaatccgtt ttgccgtcgc catcataatc acccggcgcg ggtttatcgc cgtttgcacc
       61 gaattgttcg acgcgcaaaa gattcgggct gccggaactg ttccagatat accagtaacc
      121 gtttgatgga cggaaaaccg ccacgtccgt gaagccgtcg ccattgtaat ccgccggaac
      181 gggaatatcg ccgtttgtgc cgaactgctg ggcgcgcagg gtcgttcgca gctggatata
      241 ccaagtcccg tcagacggac ggaaaacggc gtaatcggtc tttccgtcgc cgtcgtaatc
      301 gccgggcgca agaatatcat tcgcaatgcc gaaattgatt ccgctaaagc cggaatttga
      361 actgctcacc agataccacg agcccgtctc cgggcgaaaa accgagatgt cggttctgcc
      421 gtcaccgtca aagtccgccg ttttattttt cgcgggctgg actccggcta cgggtccgtc
      481 ggcgatattg ccgtcgatgt taaatgtgac gccgccccag gtttcattgt gcggagcttg
      541 atattgtttg atgcgttgat gatttgccca aatatcattt ggtatcatcg gcgagggcga
      601 cgcataaaac cagaccgaag cgacattgtc ccagcgcgcc atccaaatcg cgtccatacg
      661 gctcggcgcg ggaatattta tccaatcgcc gacggcgttg gtcggcgagc cgtaaacgcc
      721 ggaaatatag ccctgctctt ttaagcggtc agtccaacct ttcagaaacg ctttgaccgg
      781 tgtcgaacaa gcgcccgtgc cgcttacgtc gtcgtagcgc tccatgtcgt aataaagcac
      841 cgcgccttgc gtcaatccga gattattggc atcgacgata gcgatgtcgg cttcgccgcg
      901 tccctgcgtt tcggcaagtg ccgggtcgga gctgtgtttt tgacagctcg tgcaaaccga
      961 acaaggcgcc tgataaccga caatcgtcgg aatcaatccc cagcccatcg cggagacctg
     1021 attgacccaa gccgccgtca attgcggctg cgcgcatccg cgatttctgc cgctgatata
     1081 aatgtttgcg tcgtaaaacg gcgaattgtt ccaccacgtc tgcatctgcg cggcggtcgc
     1141 ggcggtgcat ttatcgaagc ctcgattcag gctgattcgg gtcgagccgt tcggcggggc
     1201 ggcgaaaacg gaattttgcg cctggagtcg ggcgttttct ttttcgagtc gggcgagttc
     1261 cttaattcgc ggcggcgtga tttctctcgc gcggttgaaa atttttgttt cctgaataca
     1321 gccggttttg aagccttcgc aatcgccctc tgttttcaaa gtccagtttc ccgagatgtt
     1381 ttcgaaactt tcgtcggaac ggcgcaggct gtgagacctt ttcgtgaacg tccagttgtt
     1441 tccgccgtcc gtggtttcat aatgaatctt cgctgtgaaa ctggaactgg tcggggcgcg
     1501 caaaagcaac tccaaatcac ggaaatcttc actttcgtaa atcaataaac cgacctgttc
     1561 cagatcggct tgctgtaaat cttcgcttct caattcgatc ggagttttcg tccagttgtt
     1621 tccgccgtcc agcgttttcg caagttcaag ccgagcattt cgcgaatcgg tcaaaattgc
     1681 caaaccggca ttttcattct gaaaataaac agccgcgatt ttttcagcgt tgtttcgcgg
     1741 caaaataatc tcacgccagg tttcgccgtc gtcgtcggtt cggaaaagag cgtcgcttgt
     1801 atgagccatc cccgattttt cgccgaaaac ctgaatgttt ttgatttgcg cacttggcgg
     1861 agtttgattt ttttgcgacg agcgttgcag cgtggtgatt atctccatat ttcggaaagg
     1921 attttcctgc gctttctgcg ccggcaaata aaaaccgaaa aaggcgattg aaaaaataac
     1981 tgccgctaaa ccgaataaag cttttctcat aaggtgatgc ctcgaaatcc gggaatttgg
     2041 aaaactcgtg aataattaaa aaagtataac tgacgcgaat gaatttttac agatttttac
     2101 tgcaactatt tcacgtattc aggcgtccat agctcaattc gtaattgaat ttgttacaaa
     2161 tcgttcgcgt gaacggtttg agcggttttt ataaacttga cttttttgta agtctagtct
     2221 atttttatgt gacaatgttt gaagtctgac cagactgtta agcttcgggc attaatataa
     2281 caagcgagaa agggagggaa attatggcac gaacggagtt atcttcatcg gcgagtcgcc
     2341 cgccggttca tcatcggatt atggcgaatt tgcaggtgca ggcgagttgg attgataaag
     2401 tgaccggaaa aacggtcttg gtcgaaggct taacggagaa tatcggtgaa accagtgctt
     2461 tggttaattt gactactctc ccgccggtcg gaagtgaagt taatcttcgg attattgacg
     2521 aagaaaaaac tttaattgaa gtgccgacgg aagtaatcag ggtcgaacgc gaccccagca
     2581 aaccattagc cgcgctctct gtcggcgaaa acttgaaaaa gtggaaaacg actgtcatga
     2641 ccgcagctca ggagtgggtc actcgtcaat ggaaacttaa ctacgaagaa gaatgggtca
     2701 actagctttt taggcttcat ttcttattca taattaaacg gagaagctat ttaaggtagc
     2761 ttctccgttt cccgtttccg accggatttt tgtaaattta tgtaaatccg cgcccgccgc
     2821 tttcccgcag caaactgata aatcgtataa tgcatctttt tatagtaatg aaaaaattca
     2881 tagctttagc tttaatcctg atttcagccg gttttatttt cgcgcaggaa acgccgccgc
     2941 agcagaaacc tctgtcgcag accgaatacg tcaggctgct ttacgatttg gaaaaaaatc
     3001 cgaaaaagcg cgacgaagtt atcgaaacgg tcagaaaacg cggaatcggt tttaccgtga
     3061 ccgacggcat caaaggttta accgtcacca aaagccgcaa cgacgcggaa ttgaagcgca
     3121 ctctggaaga agcctcgcgg cggcgtgaaa acccggcggc ttcgcagccg ccttcggaaa
     3181 aagaaagcgc ggaagtttta gccaaatcgc gcgaagcgac gctggcggcg gttgacgaga
     3241 tgccggattt cgtcgtcaaa cagatgattc aacgctcgga agcctacgcc ggaacaagca
     3301 atttcaaaaa tcgcgaccgc ttgattgtcg ccgtcagcta tcgctcggaa ggcagggaag
     3361 aatacaaaat tttaagcctg aacggcgtct tgcaggaaaa tccgaaagcg aaatcggctt
     3421 attcggaagt cggcggaacc agctcgaccg gcgaattcgt gacggttctg gcgacgattt
     3481 tcaaagccga aagcgacacg aaatttgaag tcatcgacac ggatttgatt cgtgggcgtc
     3541 gcgccgtcgt ctacggtttc gacatcacga aagacaaagc caagcagcaa atcacggcgt
     3601 ccggcatcgt cagcagctcg accttgagcg ggatgaaagg aaaaatctgg atcgaccgcg
     3661 agagctttcg cgttttgcgc gtcgaaagcg cggcgaccga gattccggcg gattttccga
     3721 ttcgcgccgc cagccgggcg attgattacg attgggtgat gatttccgag cagaaatttt
     3781 tgctgccgct gatgtccgac gtgcggctga cgtttcgcga aaatcgacag ctttacgaga
     3841 cgcgtaattt aattcgcttc aaagaatatc aaaaattcgg gtcggacgtg aaaattctgg
     3901 acagcgatga cgaagaagta acggaagaaa aaccgaatca ataaaaagaa ttcccgattt
     3961 caaatttata atctgaaatc gggaattcga atttaccagg cgagataagc gaccagagcc
     4021 ggaacgataa gccagagcaa gcctttaatc agaaaaaaca taaatcccgc aaagccaaag
     4081 cgttttatcc attggctggc gggaaatttt cttttttcgg gcgtttccgg catcatcata
     4141 aaaataactt ttttgtatga atttttcaaa ccgcttattt accgttcagg cttaattatg
     4201 aaaaaacatt tccggagatt taccgcgccg cttttttgtt ttattctttt ttcgtccgca
     4261 gtttttgctc agagcgcggc agcgccgcga gaagaaaaca aacgtgaggc tgatttggtc
     4321 gaactcgtca aactcgataa aacaataaaa ctcgatattc gctatgcccg caacgataat
     4381 tttgtcggca aaatcgtcta tacggaagcg cgcgcttttc tccagcgtcc agccgccgaa
     4441 gcgctggtgc gcgtccataa acagctgaaa aaacaaaatc tcggcttaat cattttcgac
     4501 ggctatcgcc cgtggtcggt gacgaagatt ttctgggaag tgacgcccga agacaaacga
     4561 aaatttgtcg ccaacccgac aaccggctcg aaacacaatc gcggctgcgc cgtcgatttg
     4621 agcatctacg accggaaaac cggaaaatta atcgagatgc cctcggattt cgacgaattt
     4681 accgagcgcg cttcgcccga ttatcagggc ggcacggaaa aagaaagagc gaatcgcgat
     4741 ttgctccgcc gcctgatgga agccgaaggc tttaccgtca acgccaacga atggtggcat
     4801 ttcgattaca aagactggca ggactacgcg atttacgaca tcccgttttc ggcaatcgga
     4861 agctccaggg caaaatcttc caaagcgaat ttaaagaaaa accagcctcg aaaaacaaaa
     4921 acgtcgaacc cgtaaaattc aaaatgcaaa gtgcaaaata aaaatcatcg acttaaattt
     4981 tgcactttgc attttacgtt ttgcattttc gatgcgaatt actcgatttt aatcggattg
     5041 ccttccgcgt cgagataaga aa
//
LOCUS       DASWNE010000918         1909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_242230, whole genome shotgun sequence.
ACCESSION   DASWNE010000918 DASWNE010000000
VERSION     DASWNE010000918.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242230"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..647)
                     /locus_tag="VGB68_17965"
     CDS             complement(171..647)
                     /locus_tag="VGB68_17965"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF039181.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative metallopeptidase"
                     /protein_id="HEY0051186.1"
                     /translation="MAKEFTEAPEVKAIAEKLIEIFKPELEDWGIPIRYVFCSENPKK
                     DGRECIGLARKVTGFYAYLAGEPDGMFVLETGKPAYDALSPNQKIAYVHHELCHFGIG
                     EMGNLALIPHDIEEFNQVAEVHGAYFDRLQVFADAVEQGNSDTTTRDEIISRILNR"
     gene            complement(647..835)
                     /locus_tag="VGB68_17970"
     CDS             complement(647..835)
                     /locus_tag="VGB68_17970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051187.1"
                     /translation="MDNFRNFFISVLPSSPAFYLAIERPSWFTILTAVVMPCIFFIIS
                     LGVNVLVKIYLHDRSKKD"
     gene            complement(861..1181)
                     /locus_tag="VGB68_17975"
     CDS             complement(861..1181)
                     /locus_tag="VGB68_17975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051188.1"
                     /translation="MIQKNKEARELISKQEKRIETLEAEVAAEKENSASIGKSYESAK
                     SEIALLKESNAALSRAVALNEQTIELLKEDNAKQRGLVKKANKEKWKARFAAAFLIAI
                     QLLR"
     gene            complement(1201..1641)
                     /locus_tag="VGB68_17980"
     CDS             complement(1201..1641)
                     /locus_tag="VGB68_17980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051189.1"
                     /translation="MEENLKQFFKKWLWLIVLISVLAFGGFVFWLGSTLYGYLSEASF
                     NSTVQNNRTQSQTAVTEAQNAQQKAGGFAVERQIEDGLREKVIAPKLETARRNSQNSK
                     SDLERSKKKYFDETKNTQNLSANRADNCRDLNELFANTNFQYCH"
BASE COUNT          437 a          442 c          476 g          554 t
ORIGIN      
        1 gatttttttt gagataaccg gcgacggctt tctggcacgc ttgaatagaa ttccaatgcc
       61 ccagcttttc gataatttcg gcatacgtaa ttttgccgag ccgcagggcg acggcgtccg
      121 tcagcagttt ttgatattca atgtctttag gcgaggtgcg ggaggtcatt ttagcggttc
      181 aggattcgtg aaataatttc atcgcgcgtg gtcgtgtccg aattgccttg ctcgaccgcg
      241 tcggcaaaaa cctgcagccg atcgaaatac gcgccgtgaa cttccgccac ctggttgaat
      301 tcctcgatgt cgtgcgggat gagggcgaga ttgcccatct cgccgatgcc gaaatgacag
      361 agctcgtgat ggacataagc gattttctgg ttcggcgaaa gcgcgtcgta agccggtttg
      421 cccgtttcca gcacgaacat tccgtccggc tcgccggcaa gataggcata aaagccggtg
      481 actttgcgcg ccagaccgat acattcgcgc ccgtcctttt tcggattttc cgagcagaag
      541 acgtaacgga tcggaatgcc ccagtcttca agttccggtt tgaaaatctc gatgagcttt
      601 tcggcgattg cctttacttc cggcgcttcg gtgaattctt tcgccattag tcttttttgc
      661 tgcggtcgtg cagatagatt ttcaccagaa cattaacgcc cagcgagata atgaagaaaa
      721 tacaaggcat cacgaccgcc gtcaggatgg tgaaccacga cgggcgctcg atggcgagat
      781 aaaacgccgg cgacgacggc aaaacggaaa tgaagaaatt tctaaaattg tccattgagg
      841 tgaggctgat tcgacagctt ttaacgtaat aattgaattg caatcaagaa agcggcggca
      901 aagcgcgctt tccatttttc cttgttggct tttttcacta gcccgcgctg cttggcgtta
      961 tcttctttga gcagctcaat agtttgctcg ttaagggcga cggcgcggct cagagcggcg
     1021 tttgattcct taagcaacgc gatttctgat tttgccgatt cgtaggattt tccgattgaa
     1081 gcggaatttt ccttttccgc cgcaacttcg gcttcgaggg tctcgatgcg cttttcctgt
     1141 ttgctgataa gctcgcgcgc ttctttattc ttctgaatca aaaacgcgac ggttttatct
     1201 tcagtgacag tattggaagt ttgtgtttgc gaaaagctcg ttgaggtcgc ggcaattgtc
     1261 agcgcgatta gcagaaagat tttgagtatt ttttgtttca tcgaagtatt tctttttcga
     1321 ccgctccagg tcggattttg agttttgtga gtttcggcgc gccgtttcga gcttcggcgc
     1381 gattaccttt tcgcgcaacc cgtcttcaat ttggcgttca acggcgaagc caccggcttt
     1441 ctgctgcgcg ttttgcgcct ccgtaacggc agtttgagat tgcgtccggt tgttttgcac
     1501 cgtcgaatta aaagaggctt cagagagata gccgtaaagt gtgctgccga gccagaagac
     1561 aaatccgccg aaggcgagca cactaattaa gacaatcagc cacagccatt ttttgaaaaa
     1621 ttgttttaag ttttcttcca tattgtttgt aaaagcagcc ggattcgcgc ccggcagccg
     1681 tgctatgctt cagctgataa tttgaaatag ttccggaaag aatctttcac catctcgacg
     1741 ccgatatgaa tttgtttgcg gtatttatta attaacccaa gttgctagtt ctaaagtttg
     1801 atatttgaaa acagaaaagt cctcttttag aattttgtcg ccaaacaatt tctgaaaaag
     1861 aggactcgaa atgtgcgatt gcactcttgc aatatactgt tttattgac
//
LOCUS       DASWNE010000919         2205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_242281, whole genome shotgun sequence.
ACCESSION   DASWNE010000919 DASWNE010000000
VERSION     DASWNE010000919.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242281"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VGB68_17985"
     CDS             complement(<1..288)
                     /locus_tag="VGB68_17985"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HEY0051190.1"
                     /translation="MSEENKRKDDYSYEEELRTDLDDKTTDKPQGLALHTKILIGLLV
                     GVIGGLIVNWTSGGNPNVVWTVENITRPIGQLFLNLLLMIVVPLVFSSLVVG"
     gene            complement(334..744)
                     /locus_tag="VGB68_17990"
     CDS             complement(334..744)
                     /locus_tag="VGB68_17990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEY0051191.1"
                     /translation="MNYEEASGKNIKKKVEKFEDLVAWQKARELTKEIYEVTSIGEFS
                     KDYGLRDQIRRASVSIMSDLAEGFERGKLTKFHQFISISKASCAKLRSQLYVAWDVGY
                     LSKEDFKNLMLQAREVGKIIGGLRKSVEKKRDDK"
     gene            complement(790..1656)
                     /locus_tag="VGB68_17995"
     CDS             complement(790..1656)
                     /locus_tag="VGB68_17995"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEY0051192.1"
                     /translation="MVNQAIKHVGVVVKPNHAEAWQTAGELAAWLKNHDIKLIGNPRG
                     EAEICPLEEVGVEEFQEKADLIVVLGGDGTMIAAARLLGKREIPVLGINYGSLGYMTE
                     FRIEEMFTALENILAGFYEIDRRVMLEIEHRRDAEILGTGRVLNDVVINKSVLARIIE
                     IEVKLNEQYVNSFRADGLIVSTPTGSTAYNLSAGGPIVYPSMNAVVLTPICPFTLTNR
                     PIVTPDTAEIKLRLTSEAEGVVLTLDGQIGYQMQTGDTVVICKSETNFNLVQPPNRNY
                     FDVLRNKLKWGR"
     gene            complement(1686..2171)
                     /locus_tag="VGB68_18000"
     CDS             complement(1686..2171)
                     /locus_tag="VGB68_18000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051193.1"
                     /translation="MIIAETSFAEAVKEFKKFLAKNNQPSEILWLFLEDAFSRNTKFH
                     ETHFRLKLPLPEENEKLAEKLYGIGQQRKLGVCLSAFALCEGKVCCAVLVPKDETESE
                     YMLMSPEHVKLSYAMDMPLAEAVTSTFRWKLFRLLPFKYRQGNSSDYLPSKKDLQFSR
                     V"
BASE COUNT          552 a          557 c          478 g          618 t
ORIGIN      
        1 gccgacgacc agcgacgaaa aaacgagcgg aacgacaatc atcagcagta aattcagaaa
       61 taactgcccg attggacgcg tgatattttc caccgtccaa accacattcg gattgcctcc
      121 gctggtccag ttaacgatta aaccgccgat gacgccgacc agcagaccaa tcaaaatttt
      181 ggtgtgcaaa gccaagccct gcggcttatc cgttgtttta tcgtccaaat cggttcgcaa
      241 ttcttcttcg tatgaataat cgtcttttct tttgttttct tcgctcatat tttttagtgc
      301 tgggtgctga gtcctaagtc ctgagcaaca cagttatttg tcatcacgtt ttttctcgac
      361 ggattttctt aaaccgccta ttatttttcc gacctcgcgg gcttgcagca ttaaattttt
      421 aaaatcttct ttactcaaat aaccaacatc ccaggcaaca tataactgag aacgcagttt
      481 ggcgcaggat gctttgctta ttgagataaa ttgatgaaac ttcgtgagct ttccccgttc
      541 aaatccttcg gctaaatcgg acattatcga aactgacgcg cggcgaatct ggtcgcgtaa
      601 tccataatct ttagaaaact ctccaatcga agtgacttcg taaatctctt ttgttaactc
      661 tctcgccttc tgccaggcta ctaaatcttc aaatttttcg accttctttt tgatattctt
      721 accggatgct tcctcgtaat tcataacata aataatactc agcacttagc acacagcact
      781 cagaactctc taccgtcccc attttaattt gtttcttaaa acatcgaaat aattacggtt
      841 cggcggctgg acgagattaa aattcgtttc gcttttacag atgacgaccg tgtcgcccgt
      901 ttgcatctgg tagccgattt gcccgtccag agttaaaacc acgccttccg cttcgctcgt
      961 caaacgcaat tttatttccg ccgtgtcggg cgtcacaatc gggcgattgg tcagcgtaaa
     1021 cgggcaaatc ggcgtcagca cgacggcgtt catcgaagga taaacaatcg gaccgccggc
     1081 ggaaagattg taagcggtcg agcccgtcgg cgtcgagaca atcaagccgt cggcgcgaaa
     1141 ggaattgacg tattgctcgt ttaatttgac ttcgatttcg atgatgcgcg ccagcacgga
     1201 tttgttaatg acgacatcgt tcaggacgcg tccggttccg agaatctcgg cgtcgcgccg
     1261 gtgttcgatt tcgagcatca cgcggcggtc aatctcgtaa aaaccggcga ggatattttc
     1321 gagcgccgtg aacatctcct cgatgcgaaa ttccgtcata tagccgagac ttccgtaatt
     1381 gatgccgaga accggaattt cgcgttttcc gagcagccgc gccgcggcaa tcatcgttcc
     1441 gtcgccgccg agcacgacaa tcaaatccgc tttttcctga aattcctcga cgccgacttc
     1501 ttccaaaggg cagatttccg cttcgccgcg cggattgccg ataagcttta tatcgtgatt
     1561 tttcagccac gccgccaact cgccagccgt ctgccacgcc tcagcgtgat tcggtttgac
     1621 gacgaccccg acgtgtttga ttgcctgatt aaccatttga agacaaagat tttgtcaatg
     1681 attaactaaa cgcgggaaaa ctgcaagtct tttttagaag gtaaataatc ggaagagttt
     1741 ccctgcctgt atttgaacgg taaaaggcgg aaaagtttcc agcgaaacgt gcttgtgacg
     1801 gcttcggcga gcggcatatc catcgcatag ctgagcttta cgtgttcggg cgacataagc
     1861 atgtattcag actctgtttc gtctttcggc acgagaacgg cgcagcaaac ttttccttca
     1921 cataaagcga acgctgaaag acaaacgcct aattttcttt gctgaccgat tccgtaaagc
     1981 ttttccgcca gtttttcgtt ttcttccggc agcggcaatt tcagccggaa atgcgtttcg
     2041 tgaaactttg tgtttcgaga gaaagcgtct tcaagaaaca gccataagat ttcggacggc
     2101 tgattatttt tagccagaaa ctttttgaat tcctttactg cttcggcaaa tgaagtttcg
     2161 gcgatgatca taaatttatc gactcaattt aagcacaaaa aaatg
//
LOCUS       DASWNE010000920         4462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_341974, whole genome shotgun sequence.
ACCESSION   DASWNE010000920 DASWNE010000000
VERSION     DASWNE010000920.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341974"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..1558
                     /gene="atpA"
                     /locus_tag="VGB68_18005"
     CDS             20..1558
                     /gene="atpA"
                     /locus_tag="VGB68_18005"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEY0051194.1"
                     /translation="MEAIKANEINEIIRSQIENFDASMTVSEIGAVIKVGDGIAEIHG
                     LEKVMAGELLEFPHAVRGLALNLEEDKVGCVLFGDFQKIKEGDEVRRTKRIMSVPVGD
                     ALIGRVVDALGNPIDGKGEIVTDTFNPVERIAPGIIDRQPVKEPLQTGLKAIDAMVPI
                     GRGQRELIIGDRQTGKTAVAIDTIINQRGKDVICIYVAIGQKASTVAQVVKKLEDYGA
                     MDYTIVVSATASDPAAMQFLAPYSGVAMGEFFRDNGRHALTIYDDLTKQAAAYREISL
                     LLRRPPGREAYPGDVFYLHSRLLERAAKMSDARGGGSLTSLPFIETQAGDISAYIPTN
                     VISITDGQIFLESDLFNSGVRPAINVGNSVSRVGGSAQIKAMKQVAGTLRIDLAQFRE
                     LAAFAQFGSDLDKSTQSQLERGKRLTEILKQPQYQPMPVENQVFIIWTVSNGLADDIE
                     VADLKRFEDEIFKFIESSHPAVLETLRTKRSIDDDLKASMREAVEDFKSTRWTEAKAA
                     SAVV"
     gene            1703..2482
                     /locus_tag="VGB68_18010"
     CDS             1703..2482
                     /locus_tag="VGB68_18010"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY0051195.1"
                     /translation="MSKEKLSEEIASFEGLWEGGYYEGDPLNPLAKSTYGNYGYISVL
                     HATYLRCIKPYIKPDTIALEIGPGRGAWTKTMLDAKEVWALDALSAEYNRFFEYLNHP
                     KNVKYFQVQDFECKELPENYFTYMFSFGCLCHVSFDGISEYARNIFPKLKPGSNCFWL
                     VADKKKYNEFVETEQKFDIWQGLAPKRKKLAPVRIVFETVSKVTKPTFRKTDDFAEGQ
                     GHWYDAGTERTCEMLEKIGYKVVDPDVGTSPRDPIIHFIKP"
     gene            2500..3378
                     /gene="atpG"
                     /locus_tag="VGB68_18015"
     CDS             2500..3378
                     /gene="atpG"
                     /locus_tag="VGB68_18015"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEY0051196.1"
                     /translation="MPSLLDMRRRIKSVKNTQQITKAMKMVAAAKLKRATDRVTAARP
                     FAQKMSDVLGALSARIGDDFSHPLLDSRGDEKYLIVIVTADKGLAGAFNANVIKIAQA
                     FMKENTGKSVQMMPVGRKGRDFFKRREIAFIEEYVGLTGKGRIEYGEAVEIAQNVIRA
                     FTEDESIDKVFLVYSEFKSVLSQKPSIQQLLPIPKVTGEAAQGNGAQAEYIYEQPPAE
                     IFGRLLPKQVETQIYRAILESIASEQGARMTAMDSASKNAGELISTLTLNMNRIRQAA
                     ITKEIIEVVSGAAAAQ"
     gene            3547..4020
                     /locus_tag="VGB68_18020"
     CDS             3547..4020
                     /locus_tag="VGB68_18020"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEY0051197.1"
                     /translation="MTETALPKPKWRGRFFRYAPVILWIGAIFIASSNTGSMSSTSRI
                     IRPLLEFLFPATPEEILVVYHGYIRKFAHFAEYSALAFLAARAFSGSSVKTLRNYWFA
                     FALVLVVSVATADETNQSFLASRTSSVYDVLLDSAGGLTMILFFWLYRRFFSKND"
     gene            4108..>4462
                     /locus_tag="VGB68_18025"
     CDS             4108..>4462
                     /locus_tag="VGB68_18025"
                     /inference="COORDINATES: protein motif:HMM:NF024813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY0051198.1"
                     /translation="MSFFDKVKEAAMNQFIEVIEWLDNSGDTILYRFPVAGQEIKNGA
                     QLIVRESQAAVFVFEGQAADTFGPGRYTIDGGNTPILSKLGAWKYGFNSPFKSEVYFV
                     NTKQFTDLKWGTANPI"
BASE COUNT         1104 a         1098 c         1196 g         1064 t
ORIGIN      
        1 caactatcaa ctaaaaaata tggaagcaat aaaagccaac gaaattaacg agattattcg
       61 ttcacagatt gaaaattttg atgcaagtat gaccgtttcg gaaatcggcg cggtcatcaa
      121 ggtcggtgat ggtatcgccg aaattcacgg gcttgaaaaa gtgatggcgg gcgagcttct
      181 cgaatttccg cacgccgttc gcggtctcgc cctcaacctc gaagaagaca aagtcggctg
      241 cgttttgttc ggcgatttcc agaaaatcaa ggaaggcgac gaagtcagac gcaccaagcg
      301 tattatgtcg gttccggtcg gcgacgcgct gattggtcgc gtggtcgacg ctttgggaaa
      361 tccgattgac ggcaaaggcg agattgtgac cgacaccttc aatccggtcg agcgcatcgc
      421 gcccggaatt atcgaccgcc agcccgtcaa agagccgctc caaaccggct tgaaggcgat
      481 tgacgcgatg gtgcccatcg gtcgcgggca gcgcgagctt atcatcggcg accgccagac
      541 cggaaaaacc gccgtcgcca tcgacacgat tatcaaccag cgcggcaaag acgtgatttg
      601 catttacgtc gccatcggtc aaaaggcttc gaccgtcgcg caggtcgtga aaaaactcga
      661 agattacggc gcgatggatt acacgattgt cgtatcggcg accgcttcag acccggcggc
      721 gatgcagttt ctcgcgccct attcgggcgt cgcgatgggc gagtttttcc gcgataacgg
      781 acgccacgcg ctgacgattt acgacgattt gacgaaacag gcggcggcgt atcgcgagat
      841 ttccctgctg cttcgccgtc cgccgggtcg cgaagcttat ccgggcgacg ttttctatct
      901 ccactcgcgc cttctggaaa gagcggcgaa aatgtcggac gcgcgcggcg gcggaagctt
      961 gacttctctg ccctttatcg aaacgcaggc gggcgacatc tcggcttata ttccgaccaa
     1021 cgtgatttcg attaccgacg ggcagatttt ccttgaatcc gatttgttca actccggcgt
     1081 ccgtccggca atcaacgtcg gaaactcggt ttcgcgcgtc ggcggctcgg cgcaaatcaa
     1141 ggcgatgaaa caagttgcgg gaacgcttcg catcgacctg gcgcagtttc gcgagctggc
     1201 ggcatttgct caattcggtt cggatttgga caaatcgacg cagtcgcagc ttgagcgcgg
     1261 caagcgtctg accgaaatcc tgaaacagcc gcagtatcag ccgatgccgg tcgaaaatca
     1321 ggttttcatt atctggacgg tgagcaacgg tttggctgac gacatcgaag ttgcggattt
     1381 aaaacgcttt gaagacgaaa tctttaaatt tatcgaaagc tcgcacccgg cggttctgga
     1441 aacgctccgc acgaagaggt cgattgacga cgatttgaag gcgtccatgc gcgaagcggt
     1501 cgaagatttc aaatcgacgc gctggacgga agcgaaagca gcttcagccg tagtttagaa
     1561 aaaggtcgga accgcctgcg gtagcgggcg gtttataaaa ggattaaagg ctaagggata
     1621 aaggataaat tctttgtttt acttttgcct ttatccctta gcctttatcc ttttaagagc
     1681 cgcaggcggt tctgactttt gtatgtcgaa agagaaatta tccgaagaaa ttgcgtcgtt
     1741 tgaagggctt tgggaaggcg gttattacga aggcgacccg ctgaatccgc tcgccaaatc
     1801 gacttacggc aattacggtt atatctcggt tttgcacgcg acttatttga gatgtatcaa
     1861 gccgtatatc aagcccgaca cgattgcgct ggaaatcgga cccgggcgcg gcgcgtggac
     1921 gaaaacgatg ctcgacgcaa aagaggtctg ggcgctggac gcgctttcgg cggaatacaa
     1981 ccgattcttt gaatatctga atcatccgaa aaacgtcaaa tattttcagg tgcaggattt
     2041 tgagtgcaaa gagctgccgg aaaattattt tacgtatatg ttttcgttcg gctgcctgtg
     2101 ccacgtctct ttcgacggca tcagcgaata cgcccgaaac atttttccga agctgaaacc
     2161 gggctcgaat tgtttctggc tggttgccga caaaaagaaa tacaacgagt ttgtcgaaac
     2221 cgagcaaaaa ttcgatatct ggcagggact tgcgccgaaa cgaaaaaagc tcgcgcccgt
     2281 gcggatagtt ttcgagacgg tttccaaagt gacgaaaccg acctttcgca agacggacga
     2341 ttttgccgag ggtcagggtc actggtatga cgcgggaacc gaaaggacgt gcgagatgct
     2401 tgaaaaaatc ggctacaagg tcgtcgaccc ggacgtcgga accagcccgc gcgacccgat
     2461 tattcatttt ataaaaccat agaattttga aataaaacta tgccaagctt attagatatg
     2521 cgccggcgca tcaagtcggt caaaaacacg cagcaaatca ccaaagcgat gaagatggtc
     2581 gccgcggcaa agctcaaacg cgcgaccgat cgcgtgacgg cggcgcgtcc gtttgcgcag
     2641 aaaatgtcgg acgttctcgg cgcgttgagc gcgcgcatcg gcgacgattt ttcgcacccg
     2701 ctgctcgatt cgcgcggcga cgaaaaatat ttgattgtta ttgtcaccgc cgataaaggg
     2761 ctggcgggcg catttaacgc caacgtgatt aaaatcgcgc aggcttttat gaaggaaaac
     2821 accggcaaat ccgttcaaat gatgccggtc ggcagaaagg gacgcgattt ctttaaacgc
     2881 cgcgagatag cctttatcga agaatacgtc gggttgaccg gaaagggtcg catcgaatac
     2941 ggcgaagcgg tcgaaatcgc gcagaacgtt atcagagctt tcaccgagga cgaatcgatt
     3001 gataaagtct ttctggttta cagcgaattc aaatccgttc tgtcgcaaaa gccgtcgatt
     3061 cagcaattgc ttccgattcc gaaagtgacg ggcgaagcgg cgcagggaaa cggcgcgcaa
     3121 gccgaatata tttacgagca gccgcccgcc gaaattttcg ggcgtctttt accgaaacaa
     3181 gtcgaaacgc aaatttaccg ggcgattctc gaatcgatcg cctcggagca gggcgcgcga
     3241 atgacggcga tggacagcgc gtcgaaaaac gcgggcgagc ttatcagcac gctgacgctg
     3301 aatatgaacc gcattcgtca ggcggcgatt acaaaagaaa ttatcgaggt cgtctcgggc
     3361 gcggcggcgg ctcagtaaaa gccgattttc aatcaaaacc aaagcaagcg gagcattgaa
     3421 atcaatttga ttttcttata tgcttggctt gctttttcat ttccaaatca aaagctatca
     3481 ttcggaataa tcttttacgg ctatggctta tttacatcgt tcggcgacgt aattcatcga
     3541 taaataatga ctgaaaccgc tttgccgaaa ccgaaatggc gcggacgttt ttttcgttac
     3601 gcgcctgtta ttttgtggat tggcgcgatt tttatcgctt cgtcgaatac gggctcgatg
     3661 tcgagcactt cgcgcattat ccgcccgttg ctggaatttc tttttcccgc gacgcccgaa
     3721 gaaattctcg tcgtttatca cggctacatt cgcaaattcg cgcattttgc cgaatactcg
     3781 gcgctggctt ttctggcggc gcgcgctttt tccggttctt ccgtcaaaac tctgcgaaat
     3841 tactggttcg cctttgcgct ggttttagtc gtgtcggtgg cgacggcgga cgaaacaaac
     3901 cagagttttc tcgcttcgcg gacaagctcg gtctacgacg ttctgctcga cagcgcaggc
     3961 ggattgacga tgattttgtt tttttggctt taccggcgtt tcttttccaa aaacgattga
     4021 ttgccttgtt tcaaaccctt ttcccttttc attcacgaaa ctttatgcga tatttttcgt
     4081 taaggaattc aaaggagaaa ttataaaatg agttttttcg acaaagttaa ggaagccgcg
     4141 atgaaccagt ttatcgaagt catcgaatgg ctcgataatt cgggcgatac gattttgtat
     4201 cgctttccgg tcgccggtca ggaaatcaaa aacggcgcgc aattaatcgt gcgcgaatcg
     4261 caggcggcgg ttttcgtttt tgaaggtcag gcggctgaca ctttcggtcc cgggcgctat
     4321 acgattgacg gcggcaacac gccgattctg tcgaaattgg gcgcctggaa atacggtttt
     4381 aattcgcctt tcaaatccga agtttatttc gtcaacacca aacagttcac cgatttgaaa
     4441 tggggtacgg caaacccgat ta
//
LOCUS       DASWNE010000921         2042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_278461, whole genome shotgun sequence.
ACCESSION   DASWNE010000921 DASWNE010000000
VERSION     DASWNE010000921.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278461"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="VGB68_18030"
     CDS             complement(<1..1216)
                     /locus_tag="VGB68_18030"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY0051199.1"
                     /translation="MANRLIFLIICAAIILTTLFYGAVHQPILALFYTSAALVLLFWV
                     FDSFKTGELRFSKSPLQIPLAAIILFGLVQVIPFGSLAEVSGVENISRTISHEPFATK
                     STILHLIALLIFFAAMLAFIDDAKRLRKIVMVITIFGFLYAFYSILQAVLSPSRIYGI
                     YEPRFATPFGSFVNRHNFAAFMEMSLAVPLGLIFVGAVPRDKRLLYVTAIGLMGIALL
                     LSGSRGGLVALLAEIFFLIILTTKTRGYGQIALKIGLAVALFATIVVGSVLIGGESSL
                     TRFAETATSADITTNRTHIWNVTLSVIKNNPLLGAGLGAFPQVYTTFDTLNGMERVEQ
                     AHNDYLQVLADAGIVGLLIGAFFVFWLFRDGLRNVKTHNTFRRGVAVGALAGCFAIFV
                     HSIFDFVLHTTAI"
     gene            complement(1366..>2042)
                     /locus_tag="VGB68_18035"
     CDS             complement(1366..>2042)
                     /locus_tag="VGB68_18035"
                     /inference="COORDINATES: protein motif:HMM:NF014116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HEY0051200.1"
                     /translation="LFYATKERAADSLRVWNTLTPEQKTENESDARIIAQAFFEKKYF
                     RSAVGFTREVGIETDAQAEKIQNAGFESSIGKENDSYFNWKVIPTEKVDVRLDPSQKR
                     SGNRSLRIVFNGYAAAQYYQVYQIVAVEPNAKYRLNFWLRTENLKSAGNPLIEIVNAN
                     DEKPVKASEAFSDGTNEWQEMKIEFVAPPNAEGVTVRAARAFCGNQCPLFGSIWLDDF
                     SISRVN"
BASE COUNT          524 a          533 c          540 g          445 t
ORIGIN      
        1 aaatcgccgt cgtgtgcagc acgaaatcga aaatgctgtg gacgaaaatg gcaaaacagc
       61 ccgccagtgc gccgaccgcg acgccgcgcc ggaacgtgtt gtgagttttg acgtttcgca
      121 aaccgtcgcg gaataaccag aaaacgaaaa atgcgccgat gagcaagccg acgattccgg
      181 cgtcggctaa aacctgcagg taatcgttgt gcgcctgctc gacgcgctcc attccgttga
      241 gcgtgtcgaa ggttgtgtaa acctgcggaa acgcgccgag tccggcgccg agcagcggat
      301 tgtttttaat cacgctcaaa gtcacgttcc agatgtgcgt gcggttggtc gtaatatcgg
      361 cggaagtggc ggtttcggca aagcgcgtca gcgaagattc gccgccgata agcaccgagc
      421 cgacgacgat ggtcgcaaac aaagcgacgg ctaaaccgat tttcaaagca atctgcccgt
      481 agcctctggt tttcgtggtc aggattatca gaaaaaatat ctccgccagc agcgcgacca
      541 gaccgccgcg cgagccgctc aataaaagcg cgatgcccat cagcccgatt gccgtcacgt
      601 aaagcaggcg tttatcgcgc ggaaccgcgc cgacaaaaat caaaccgagc gggacggcga
      661 gcgacatttc cataaacgcc gcgaaattat gccggttgac gaacgagccg aacggcgtgg
      721 cgaaacgcgg ctcgtaaatt ccgtaaatgc gcgacgggct tagaacggct tgcagaatcg
      781 aatagaaagc atacagaaaa ccgaaaatcg taatcaccat aacgattttc cgcagccgtt
      841 tggcgtcgtc gataaaggcg agcatcgcgg cgaaaaagat taaaagagcg attaaatgca
      901 aaatcgtcga tttggtggca aacggctcgt gcgaaatcgt tcgcgaaatg ttctcgacgc
      961 cggagacctc agccagactg ccgaacggaa ttacctgcac cagcccgaaa agaataatgg
     1021 cagccagcgg aatttgcagc gggcttttgc tgaaacgaag ctcgccggtt ttgaaggaat
     1081 caaaaaccca gaacagcaaa acgagcgccg cgctcgtgta aaaaagcgcg agaatcggct
     1141 ggtgaaccgc gccgtaaaag agggtcgtca aaataatcgc cgcgcagatg attaaaaaaa
     1201 taagcctgtt cgccataaaa atttttcgcc acagagagaa attcaaacag gatggacagg
     1261 atttacagga taatttacag ctgagaacat tgagctttct tttttatcct atccatcctg
     1321 tctatcctgt ttaaatatcc gcgttcatct gtgtaaatct gcggttcaat tcacccggct
     1381 aatcgaaaaa tcgtcgagcc aaatcgaacc gaaaagcggg cattgatttc cgcaaaaagc
     1441 gcgcgccgcg cgcaccgtta cgccttcggc attcggcggc gcgacgaatt cgattttcat
     1501 ttcctgccat tcgtttgttc cgtcggaaaa tgcctcgctc gccttgacgg gcttctcgtc
     1561 gttggcgttg acgatttcaa tcaaaggatt tccggcgctt tttaaatttt cggtgcgcag
     1621 ccagaaatta agacgatatt tggcgttcgg ctcgaccgcc acgatttgat aaacctggta
     1681 atactgggcg gcggcgtaac cgttaaaaac gatgcgcagg ctgcggtttc cgctgcgctt
     1741 ttgcgacggg tcgagccgga catcgacctt ttccgtcggg attactttcc agttgaaata
     1801 agaatcgttt tccttgccga ttgacgattc gaaaccggca ttttgaattt tttccgcctg
     1861 cgcgtccgtc tcgattccga cctcgcgcgt aaagccgacc gccgagcgaa aatatttttt
     1921 ctcgaaaaac gcctgagcga ttatacgcgc gtcgctttcg ttttccgttt tctgttccgg
     1981 cgtcagcgtg ttccagacgc gcaggctgtc ggcggcgcgt tccttggtgg cgtaaaataa
     2041 ag
//
LOCUS       DASWNE010000922         1681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_562550, whole genome shotgun sequence.
ACCESSION   DASWNE010000922 DASWNE010000000
VERSION     DASWNE010000922.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562550"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VGB68_18040"
     CDS             complement(<1..362)
                     /locus_tag="VGB68_18040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051201.1"
                     /translation="MNGFKKYALAGAVAASLLFVLIHFFNNSKITDSSQIAEQTLIEN
                     DSPATPANTGAAAIDKEKIDDAYGKLPLHFEPNHGQTDAQVKFLARGQGYALFLTEKE
                     AVLSLQKRGKTKAQDKRAV"
     gene            complement(497..1381)
                     /locus_tag="VGB68_18045"
     CDS             complement(497..1381)
                     /locus_tag="VGB68_18045"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY0051202.1"
                     /translation="MSRSSNDRRRRDSRPNNRDKARSNINRGKEREFKRELAEKSSAK
                     GEPQYTMQQKRAVENLLSGIGTPEPQPLVPDDFQIEALEAIETQDVLVTAPTGSGKTW
                     IAREEIRRLLENGQRAWYTTPLKALTNSKYMEFSSEFGAENVGILTGDRKENWDAPLI
                     VGTTEIYRNQLFDALRQGEQVGTDFVILDEAHYLADEDRGHVWEEAIILSPPRIRLLL
                     LSATIGNADEFARWIEEVRGVRVRVINRAGSRPVELRAAFLAPDLRLFPLFNEQGKFN
                     SELERFAQEENRFERRRH"
BASE COUNT          391 a          400 c          439 g          451 t
ORIGIN      
        1 actgcgcgct tgtcctgcgc tttggttttt ccgcgttttt gcaaagacaa aacggcttcc
       61 ttttcggtca gaaaaagcgc gtagccctgc ccgcgggcga gaaattttac ctgcgcgtcg
      121 gtttgcccgt gattcggttc gaaatggagc gggagcttgc cgtaagcgtc gtcgattttt
      181 tctttatcaa tcgccgccgc gccggtattt gcgggcgtcg ccggagaatc gttttcgatt
      241 aatgtctgtt cggcgatttg tgaggaatcg gtaatcttag agttattaaa aaaatgtatt
      301 aaaacaaaaa gcagcgaggc ggcaaccgcg cccgccagcg catatttttt aaagccgttc
      361 ataatgagaa accccgtcag aaatatgaag ataatttatc aaccgctttt acagtgtcga
      421 aattatctcg aaaaagacag agggttatga agtttttgag atttctattg aggtttttat
      481 atcgttaaat tttcaatcaa tgtcttcgcc gctcgaaacg attttcttcc tgcgcgaaac
      541 gctcaagctc ggaattaaat ttcccctgct cgttaaataa cggaaaaagg cgcaaatccg
      601 gcgcgagaaa ggcggcgcga agctcgaccg ggcgcgaacc ggcgcgattg atgacgcgga
      661 cgcggacgcc gcgcacttct tcaatccagc gggcaaactc gtcggcgttt ccgatggtcg
      721 ccgacagaag caaaaggcga atgcgcggcg ggcttaagat aatcgcttct tcccagacgt
      781 gaccgcggtc ttcgtccgcc agataatgcg cttcgtccag aatgacgaaa tccgtgccga
      841 cctgctcgcc ctgccgcaga gcgtcaaaaa gctgattgcg ataaatttcc gtggtgccga
      901 cgattaaagg cgcgtcccaa ttctctttcc ggtcgccggt cagaatgccg acgttttccg
      961 cgccgaactc cgaggaaaat tccatatatt tggaattggt cagagccttt agaggcgtcg
     1021 tgtaccaggc gcgctgccca ttttctaaaa ggcgtcggat ttcttcgcgc gcaatccagg
     1081 tcttgccgct gccggtcggc gcggtgacca gaacgtcctg cgtttcgatg gcttcgagcg
     1141 cctcgatttg aaaatcgtcc ggaaccagcg gctgcggctc gggcgttccg atgccgctca
     1201 aaagattttc gacggcgcgt ttttgctgca ttgtatattg cggctcgcct tttgcggaac
     1261 ttttctctgc aagttcgcgt ttaaattcgc gttcctttcc acggttgatg ttggaacgcg
     1321 ctttatcgcg attgttcggg cgcgaatcgc gccgccggcg gtcatttgat gaacgagaca
     1381 taaaacgatt gtaaatgttt tgttgtcaat tgttaatagt ttttttgtca gtggtaagcg
     1441 gaaaacgtta agcggtaagt ttgttatatg gtaattgcag cttaacgttt agcacttacc
     1501 gtttagcact gattaacatt tgtttccaat cgccgttcaa agatggtaaa atagcaaatt
     1561 gcttcgcaac gatttttcgg agcgaagcgt ctaagaaaga cggcgagcag tccgcaagcc
     1621 ggtctcggga acaagggttt taaatatttg tatggaagaa aaagagcttt ttcaggatta
     1681 c
//
LOCUS       DASWNE010000923         3561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_595969, whole genome shotgun sequence.
ACCESSION   DASWNE010000923 DASWNE010000000
VERSION     DASWNE010000923.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595969"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..666)
                     /locus_tag="VGB68_18050"
     CDS             complement(151..666)
                     /locus_tag="VGB68_18050"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEY0051203.1"
                     /translation="MNKSGGKDTNMNPQFLTAEELGIRLEIAGNLAIWEASPIYKHQK
                     AVDRIRDSFKKTEEDGSVCECLDVADVYVQFPDGSLKRPDISVFCREPEEEEEAIRQV
                     PEAVVEVISKKYEAKDLEISPPIYLANGVKDVVVFNPYTNEVLHFREGETRNLVSPVE
                     IEFECGCSCTV"
     gene            complement(670..1332)
                     /locus_tag="VGB68_18055"
     CDS             complement(670..1332)
                     /locus_tag="VGB68_18055"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEY0051204.1"
                     /translation="MNSNTEQDYNLEIEEPAEETPAGDTRRFNTEGERVRVEVRDETE
                     NPKQNVKSQRETELENRLKEETMRREAAEAKLVGVQAKFEEVKTDMERETQEMRSRMQ
                     KSLEDRAKQGQFSFLTALLPVLDNLNRAIQASETDASFEHLSDGVKGTARSFEQALMS
                     VGVEAIPATGMDFNPELHEAIDMIPVEPEQDGKITAEYSRGYKFGERLLRPSKVQVGR
                     GK"
     gene            complement(1524..>3561)
                     /locus_tag="VGB68_18060"
     CDS             complement(1524..>3561)
                     /locus_tag="VGB68_18060"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0051205.1"
                     /translation="RTIQVLSRRTKNNPVLIGEPGVGKTAIVEGLAQRIVSGDVPETL
                     KNKKLVGLDLGAMLAGAKYRGEFEDRLKAVLREIEKSDGQIILFIDELHTLVGAGASE
                     GAIDASNMLKPALARGMLRCVGATTLNEYQKHIEKDKALERRFQQVYVGEPNVEDTIA
                     ILRGLKERYEVHHGVRIKDSAIVAASTLSNRYITDRFLPDKAIDLIDEAASRLRIEID
                     SLPQEIDVLEREILQLEIERQALSRETDEKSKERLADIERRIADLKEKSGAMKAKWQS
                     EKEEIEKMRGAKEQLEEAKLQLEQARQAGDLNKAAELQYGRIPELEKLLGEEQTRLAD
                     LQKDGVYLKEEVDEEDVAEVVAKWTGVPVSKMLEGEMQKLVTMEDKLRTRVIGQDEAL
                     EAVSNAVRRARAGLQDPNRPVGSFIFLGPTGVGKTETAKALGEFLFDDERAMIRLDMS
                     EYMEKHAVARMIGAPPGYVGYDEGGQLTEAVRRRPYSVVLFDEIEKAHPDVFNVLLQI
                     LDDGRLTDSKGRVVDFKNTVLIMTSNLGSREILAVGDDQNQVREAVQQVLRDYFKPEF
                     LNRVDDIVIFGQLSRGEIEKIIDVQLEKLRKNLAERGITIVLEDSAKQLLASEGYDPT
                     FGARPLKRAIQTMIQNPLAVKLLKGEIAGGQTVRVTADGGSLNFTPQTTATASA"
BASE COUNT          747 a         1017 c          814 g          983 t
ORIGIN      
        1 tgtaaccttt aaaagtagtt acttcatata actcaaaaca tttagttctt tctttcagtt
       61 aattaataat ttcggattta ttcatttatt tcggtgttct cctcagattt gttagcacaa
      121 ttgcctcacg agctttcaca aattctactt ttaaaccgta cagctacaac cgcattcaaa
      181 ttcaatctcg accggcgaaa ctaaatttcg cgtttcgccc tcgcggaaat gcaaaacctc
      241 atttgtataa ggattaaaaa cgaccacatc ctttactccg tttgccagat aaatcggcgg
      301 cgagatttcc aaatccttcg cctcgtattt tttgctgata acttcgacga cggcttcggg
      361 aacttgccta atcgcttctt cctcttcttc gggttcacga cagaaaacag aaatatcagg
      421 gcgcttgagc gagccatcgg gaaactggac gtaaacatcg gcgacgtcaa gacactcgca
      481 aacacttccg tcttcctcag tttttttgaa agaatcacga attcggtcaa ctgctttttg
      541 atgtttatag attggcgaag cttcccaaat cgctaggttt cccgcaattt ccaatctgat
      601 gccgagttct tccgcagtca aaaactgtgg attcatattc gtgtcctttc cgccggattt
      661 attcattctt tactttccgc gaccgacctg gactttcgac gggcgcaaga ggcgctcgcc
      721 gaatttgtag ccgcgcgagt attccgccgt gattttgccg tcctgttccg gctcgaccgg
      781 aatcatatcg atcgcctcgt gcagctccgg gttaaaatcc attccggtcg ccggtatcgc
      841 ctcgacgccg acgctcatca aagcctgctc aaaagaacgc gccgtgcctt tcacgccgtc
      901 cgataaatgc tcgaacgaag catcggtctc gctcgcctga atcgcgcggt tgagattgtc
      961 cagaacgggc agcagcgcgg tcaagaagct gaattgtccc tgtttggcgc ggtcttccag
     1021 agatttctgc atccgcgagc gcatttcctg cgtttcgcgc tccatatcgg ttttgacttc
     1081 ttcaaatttc gcctggacgc cgacgagctt ggcttcggcg gcttcgcgcc gcatcgtttc
     1141 ttccttcagg cgattttcca gctcggtctc gcgctgtgat ttgacgtttt gtttcgggtt
     1201 ttcggtttcg tctctgacct cgacgcgaac gcgctcgcct tcggtgttga agcggcgcgt
     1261 gtcgcccgcg ggcgtttctt ccgccggttc ttcaatttcc agattgtaat cctgctcagt
     1321 attgctgttc atatttttct tcttaaacca actcataaat tttgataaac ggattgctga
     1381 taaattagtc taacaaacca tctatcagaa gaaaatgatg aacgcgaaac gcggaacgat
     1441 gaattgcagt ttctaaatat cgaattcatc gtttatcgtt ccgcgttcat catttctttt
     1501 tatccgtctg tcctgttaaa aacttacgcc gaagcggtcg ccgtcgtctg cggcgtgaaa
     1561 ttcaaacttc cgccgtccgc cgtcacgcga accgtctgcc cgccggcgat ttcgcctttc
     1621 aaaagcttga cggcgagcgg attctgaatc atcgtctgaa tcgcgcgttt taaaggtcgc
     1681 gcgccgaaag tcgggtcgta gccttcgctc gccagcagtt gtttcgccga atcttcgagc
     1741 acaatcgtga tgccgcgctc ggcgagattt tttcgcaatt tttccagctg cacgtcgatg
     1801 attttttcga tttcgccgcg cgacagctga ccgaaaatca cgatgtcgtc gacgcggttc
     1861 aaaaattccg gtttgaaata atcgcgcaga acctgctgga cggcttcgcg cacctgattc
     1921 tggtcgtcgc cgacggctaa aatctcgcgc gagcctaaat tcgaagtcat aatcaaaacc
     1981 gtatttttga aatccacgac gcgacctttc gaatcggtca accgaccgtc gtcgagaatt
     2041 tgcaataaaa cgttgaaaac gtccgggtgc gctttttcga tttcgtcgaa taaaacgacc
     2101 gaatacggac gacgacggac ggcttcggtc aattgtccgc cttcatcgta gccgacgtat
     2161 cccggcggcg cgccgatcat ccgcgcgacc gcgtgtttct ccatatattc agacatatcg
     2221 aggcgaatca tggcgcgctc gtcgtcaaat aaaaattccc ctaaagcttt cgccgtctcg
     2281 gttttaccga cgcccgtcgg acctaaaaag ataaacgagc cgacggggcg attcgggtcc
     2341 tgcagaccgg cgcgagcgcg gcgaacagcg tttgagacgg cttcgagcgc ctcgtcctga
     2401 ccgataacgc gcgttcgcag cttgtcctcc atcgtcacca atttttgcat ctcaccttcg
     2461 agcatcttgg aaacgggaac gcccgtccat ttcgcgacga cttccgcgac gtcttcctcg
     2521 tcgacttctt ctttcagata aacgccgtct ttctgcaaat cggcgagccg cgtctgttct
     2581 tcgcccagaa gcttttcgag ttcgggaatt ctgccgtatt gcagctcggc ggctttgttt
     2641 aaatcgcccg cctgccgcgc ctgttcgagc tgtagcttcg cttcttcgag ctgctctttc
     2701 gcgccgcgca tcttctcgat ttcttctttt tccgactgcc atttcgcttt catcgcgccg
     2761 gatttttcct ttaaatcggc gatgcgtctt tcgatgtcgg cgagccgttc tttggatttt
     2821 tcgtcggtct cgcgcgaaag cgcctgacgc tcgatttcca gttgcagaat ctcgcgctcc
     2881 aaaacgtcga tttcctgcgg caggctgtcg atttctatgc gcagacgcga agccgcttcg
     2941 tcaatcaaat caatcgcttt gtccggcaaa aatctgtccg taatataacg gttcgacaaa
     3001 gtcgaggcgg cgacaatcgc cgaatccttg atgcggacgc cgtgatgaac ttcgtagcgt
     3061 tccttcaatc cgcgcaggat ggcaatcgtg tcttcgacgt tcggctcgcc gacgtaaacc
     3121 tgctggaaac gacgttccag agctttatct ttctcgatgt gtttttgata ttcgtttaaa
     3181 gtcgtcgcgc cgacacagcg cagcattccg cgcgccagcg ccggctttaa catattcgaa
     3241 gcgtcaatcg cgccttcgga cgcgcccgcg ccgaccagag tgtgcagctc gtcgataaat
     3301 aaaataatct gcccgtccga tttttcgatt tccctcaaaa cggctttcag acggtcttca
     3361 aactcgcctc gatatttcgc gcccgccagc atcgcgccca aatccaagcc gacaagcttt
     3421 ttatttttca aagtttccgg cacgtcgccc gaaacgattc gctgcgccaa accttcgaca
     3481 atcgccgttt taccaacgcc cggctcgccg attaaaaccg gattgttttt cgttcttctc
     3541 gataaaacct gaatcgtgcg g
//
LOCUS       DASWNE010000924         1677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_65978, whole genome shotgun sequence.
ACCESSION   DASWNE010000924 DASWNE010000000
VERSION     DASWNE010000924.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65978"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            530..901
                     /locus_tag="VGB68_18065"
     CDS             530..901
                     /locus_tag="VGB68_18065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0051206.1"
                     /translation="MSIRVLIVEDNDDYRDLVDFFLKSRGFEVIIATNGVEAVELSNS
                     EKPHIILMDINMPIVDGLEATRIISQNLSTKHIPIVAVSANCAGKMGEKVLNAGAVGC
                     VQKPVDIESLPDLILNYALND"
     gene            complement(995..>1677)
                     /locus_tag="VGB68_18070"
     CDS             complement(995..>1677)
                     /locus_tag="VGB68_18070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0051207.1"
                     /translation="FIDALAPDDRVAVIEFYDKVNLRNDFTIDRQITANSIRVSNGRG
                     KTQLYKALDFALEKLSKEGKRRKAIIVLTDGVDTALRDKDRDFLSSVKESEMATAIKP
                     EINETLNKILNNADRQGVTIYPLALPTGDPSKLAEPTPIQTAMYQAARERLKILANRT
                     GGTLNAINRLEEMGRFYALVAADIRSLYTVEYQPANDKRDGKWRAIKIEVANPELIAR
                     TREGYFGR"
BASE COUNT          426 a          389 c          401 g          461 t
ORIGIN      
        1 gggattaaaa gagcgaattt cccggcacac ttcaagactc gactggtcgc cgaaacgatt
       61 gtctaaaacg atggcgtcca attttttatt tcttgcgtga tgcaaacaat catccaacga
      121 atcgcaggac gttacttcaa aatccgcctg cttgaatata aacgttaaaa gttcgagatt
      181 atcctgatta tcatccacac aaaggattgt ctgttttgag ttttgcatta ttattcgcta
      241 tttaataatt gctcaggaac cgatttgaaa taaaatccgt cgtctgggaa gcaattaaaa
      301 ccttcgataa attattggtg tatgcaagaa atttgcataa ttgacgtgtc tgattgtagc
      361 acataaagta tacgtccgct atcgtcttaa cctctatccg ctcaataatc aagattcccc
      421 gccaataaaa atcggttcct cgtcaataaa aaataattta aagctcgcgt ttatggtaag
      481 atattcgctt gcccgtttaa ataaagggta aatagtcaag gggtgactta tgagcataag
      541 ggtattgatt gtagaagata atgatgatta ccgggatttg gtcgactttt ttctgaaatc
      601 gagaggattt gaagtcatca tcgccaccaa cggagtcgaa gcggtcgagc taagcaattc
      661 cgaaaagccg catattatcc tgatggatat aaatatgccg attgtcgacg gcttggaagc
      721 gaccagaata atcagccaga atctttcgac caaacacatc ccgatagttg cggtatcggc
      781 aaactgcgcc ggcaaaatgg gcgaaaaggt attaaatgcc ggcgcggtcg gctgcgttca
      841 aaagccggtc gacatcgaaa gccttcccga cttaatcctg aactacgcct tgaatgactg
      901 aatttaaaaa agccgagccg gagcgttttt gctccggctc ggctttttta aatttggaag
      961 ttcagcttaa cgcgggattt tctcctgaaa atcattatct gccgaaatag ccttcgcgcg
     1021 tgcgggcgat caattccgga ttggcgactt cgatttttat cgctcgccat ttgccgtcgc
     1081 gtttgtcgtt ggcgggctgg tattcgaccg tgtagagcga gcgaatgtcc gccgcgacca
     1141 gggcgtaaaa cctgcccatt tcttcgagcc gattgatggc gtttaaagtt ccgcccgtgc
     1201 ggtttgccag aattttcaga cgctcgcgcg ccgcctgata catcgccgtt tgaatcggcg
     1261 tcggctcggc gagcttgctc gggtcgccgg tcggcagcgc cagcggataa atcgtcacgc
     1321 cctgacggtc ggcgttattc aaaattttat ttaaggtttc attaatttcc ggtttgattg
     1381 ccgtcgccat ctcggattct ttgacgctcg acagaaaatc gcggtctttg tcgcgcagcg
     1441 ccgtatcgac gccgtcggtc aaaacgataa tcgccttgcg gcgttttcct tctttcgaga
     1501 gtttttcgag cgcgaaatcg agcgctttat aaagctgcgt cttgccgcgc ccgttcgata
     1561 cgcgaattga attggcggta atctgacgat caatcgtaaa atcgtttcgc aaatttacct
     1621 tgtcgtaaaa ttcgatgacg gcgacgcggt cgtccggcgc gagcgcgtcg ataaagc
//
LOCUS       DASWNE010000925         1614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_489586, whole genome shotgun sequence.
ACCESSION   DASWNE010000925 DASWNE010000000
VERSION     DASWNE010000925.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489586"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="VGB68_18075"
     CDS             <1..1071
                     /locus_tag="VGB68_18075"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEY0051208.1"
                     /translation="SPAGQLIKNLQAQNVNVSEIVFVTTLVAKDSVSIILTTGNKQKA
                     FTQNIPKENLSKLVFEFRSAVTDIGKNPKIAGKKLYDLLLKPLEIELAEAKATKIVWS
                     LDGVLRYLSIPALYDGKNYIVQRFASIQITFASDEKVLFPKVSNPTAIGLASSKPFEN
                     LSSLPAAKNELDCIFDDGRKLIINSTCAKGLIKGKKVADEDFTQEMFEVALKNYQLIH
                     FTSHFVLQAGDNSKSFLLLGGGKNRKYTMQEFSRQQLDNVEIMIMSACDTANFSPDGA
                     EFESFATMAQKQGAKAILGTLWSVADISTSKFMREFYWFYQIKKTDKAEAIRKAQIIV
                     AADKNFSHPFFWAPFVLFGNWK"
     gene            1283..>1614
                     /locus_tag="VGB68_18080"
     CDS             1283..>1614
                     /locus_tag="VGB68_18080"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEY0051209.1"
                     /translation="MNNPYFIIEAKGEKAPLILSVPHSGTDFPDELKAHYRAEQAAQI
                     DDTDWYVHDLYNFAPDLGVTVIHAGYSRWVIDLNRSPESAPLYTDGRIITGLTPATDF
                     LGNEIYVD"
BASE COUNT          461 a          374 c          385 g          394 t
ORIGIN      
        1 tctcccgccg gacagctgat taaaaatcta caggcgcaaa acgtcaatgt ctccgagatt
       61 gttttcgtca ccacgctggt cgccaaagat tccgtcagca taattctgac gaccggtaac
      121 aagcagaaag ctttcacgca aaatattccg aaagaaaatt tgagcaagct ggttttcgag
      181 tttcgcagcg cggtgacgga catcggcaaa aatccgaaaa tcgccggaaa aaagctttat
      241 gatttgctgc tcaagccgct ggagatcgag ctggcggaag ccaaagcgac aaaaatcgtc
      301 tggtcgctcg acggcgtttt gcgctatctc tcgattccgg ctttatatga cggcaaaaat
      361 tatatcgtgc agcgctttgc cagcattcag attactttcg ccagcgacga aaaggtgctt
      421 ttccccaaag tttcgaatcc gacggcaatc ggcttggctt cgtccaagcc gtttgaaaat
      481 ttatcgagcc tgccggcggc gaaaaacgaa ttggactgca tttttgatga cggcagaaag
      541 ctcatcatca actcgacctg cgccaaaggg ctgattaagg gaaaaaaggt ggcggacgaa
      601 gactttacgc aggaaatgtt tgaagtggcg ctgaaaaatt accagttaat tcattttacg
      661 agtcatttcg ttttgcaggc gggcgataat tcaaaatcct ttctgctgct cggcggcggg
      721 aaaaacagaa aatacacgat gcaggaattt tcccgccagc agctggataa cgtggaaata
      781 atgattatgt cggcttgcga cacagccaat ttcagcccgg acggagccga gtttgaaagc
      841 tttgcgacga tggcgcagaa acaaggcgca aaagccattt taggaacgct ctggtcggtc
      901 gccgatattt cgacctccaa gtttatgcgt gaattttact ggttttatca aatcaaaaaa
      961 acggataaag ccgaagccat cagaaaagct cagattatcg tcgccgccga caaaaatttt
     1021 tctcatcctt ttttctgggc gccgttcgtt ttgttcggca actggaaata agaccgcagc
     1081 aatcgataaa tttaactcac agccgaatta tctcgcgata atagattaca aatttcaaaa
     1141 tcgaatttaa cgtcggcaaa tcgtcgtccg actttttatt gtcgtgcgga tttgaaattt
     1201 gaaatttgaa atatcaacaa agccgcaaat tatctataat gttctcgacg ccggcgcccg
     1261 cagaaaacga aaaaccgaag taatgaataa tccatatttt ataatcgaag ctaaaggcga
     1321 aaaagcacct ttgattttga gcgttccgca ttcgggcacc gattttccgg acgagctgaa
     1381 ggcgcattat cgcgccgagc aggcggcgca gattgacgac acagactggt atgttcacga
     1441 tttgtataat tttgcgccgg atttgggagt taccgtgatt cacgccggat acagccgctg
     1501 ggtgattgat ttaaaccgca gcccggaaag cgcgccgctc tacacggacg gcagaatcat
     1561 cacgggactg acgccggcga ccgattttct cggcaatgaa atttacgtcg ataa
//
LOCUS       DASWNE010000926         2304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_278542, whole genome shotgun sequence.
ACCESSION   DASWNE010000926 DASWNE010000000
VERSION     DASWNE010000926.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278542"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1693)
                     /locus_tag="VGB68_18085"
     CDS             complement(<1..1693)
                     /locus_tag="VGB68_18085"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY0051210.1"
                     /translation="MQKFLRWLDEIARVNHENRLAGWLERIAFVFLVLMVLSAPHSIA
                     ATQTSWILGMLAWLIRLFLKPRPKFIRTPLDLALWTFFGWSVLTCAFSYAPDISVHSL
                     RGTALFLIFYFVVSNVRTRRAVYFLAFAMIFSCMVNVFLTPVQRIVGRGVEIHGVRAE
                     SALAKALLYEGDTLLEANRKKIKTPEDLVAEIERNETTKVRFYRPDFYFAVDVRRENL
                     LVGASALEKLGVESWKKSRNWRSTGFYGHWTTYAEVLQLIASLAFGLFVALIAGSKFF
                     DTKAQRESEQDLQDAKIFPGFRSPSLVALLLLLCLGGMLFALLLTATRASQAAFLVST
                     FVVVLLNGNRRMMLALAAIVLPLALGGLIFLQQSRNVGFIDEKDDSTRWRQTVYREGF
                     DLWTRNPRHFLLGVGMDSIKRYAGEWHLFDDGRLPMGHFHSTPLQLLVERGLFAFLLW
                     LWILRLYGKMLWRGLKAPDSESGAFNAEPEARKNKESNFAFGIPYSILEKGILLGCSG
                     GLIGFFTSGLVHYNLGDQEVAMIFFLLMGLSVKLIQMKNEAEARASARAESSWQSP"
     gene            1764..>2304
                     /locus_tag="VGB68_18090"
     CDS             1764..>2304
                     /locus_tag="VGB68_18090"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051211.1"
                     /translation="MSQENLRDDLSIKTEINLYYDMFVPETAKGRAPLLVAVHGYGAH
                     KRYMMREARAVAPDGFAIVSLQAPHQHFRPTDSGYKVGFGWLTDYKAQESVRLHHNFV
                     LGVIERLSGEDLIDPNRIYLYGFSQACALNFRFAFTFPDVARGVIGISGGIPGDLETS
                     EIYQPTKADVFYLYNDDDEF"
BASE COUNT          591 a          632 c          585 g          496 t
ORIGIN      
        1 gtggcgactg ccaactgctt tccgcccttg ctgacgcgcg ggcttccgct tcgtttttca
       61 tttgaatcag tttgacgctc aagcccatca gcagaaagaa aatcatcgcc acttcctggt
      121 cgccgagatt gtagtgcacc agcccgctcg taaaaaatcc aatcagaccg cctgaacagc
      181 cgagcaaaat tcccttttcg aggatggaat acggaatgcc gaatgcgaaa ttcgattctt
      241 tatttttccg agcttcaggt tcggcattga aagctccgga ttcggaatcc ggagctttca
      301 agccgcgcca aagcattttt ccgtaaagcc gcaaaatcca gagccagagc agaaacgcga
      361 ataatccgcg ctcgaccagc agttgcagag gcgtggagtg aaaatgtccc atcggcagcc
      421 tgccgtcgtc aaacagatgc cactcgcccg cgtaacgctt gattgaatcc atcccgacgc
      481 cgagcagaaa atggcgcgga ttgcgcgtcc acaaatcgaa gccttcgcga tagacggtct
      541 gccgccagcg cgtcgaatcg tctttttcgt cgataaaacc gacattccgg ctctgctgta
      601 aaaagattaa tccgccaagc gcgagcggca aaacaatcgc cgccagcgcc agcatcatcc
      661 ggcgattccc gttcagcaaa acaacgacaa aagtcgaaac cagaaacgcc gcctgcgaag
      721 cgcgcgtcgc cgtcaaaaga agcgcgaaaa gcattccgcc cagacaaagc aaaagcagga
      781 gagcgacgag agacggagaa cgaaaaccgg gaaatatctt tgcatcctgt aaatcctgtt
      841 ctgattctct ctgcgccttt gtgtcgaaaa atttgcttcc ggcgattaaa gcgacaaaaa
      901 gcccgaaggc gagcgaggcg atgagctgca aaacttccgc gtatgtcgtc cagtgcccgt
      961 aaaatccggt cgagcgccag ttgcggcttt ttttccagct ttcgacgccg agcttctcca
     1021 gcgcgctcgc gccgacgagc agattttcgc gccggacatc aaccgcaaaa taaaaatccg
     1081 gtcgataaaa tctgactttc gtcgtttcgt tgcgctcgat ttccgcgacc aaatcttcgg
     1141 gcgttttgat ttttttccgg ttcgcttcca gcagcgtgtc gccttcgtaa agcagggctt
     1201 ttgccagagc gctctcggca cgaacgccgt gaatttccac gccgcgtccg acgattcgct
     1261 gaaccggcgt gagaaaaaca ttgaccatac aggaaaaaat cattgcgaaa gccagaaaat
     1321 agacggctcg ccgcgtccgc acgttgctga cgacaaaata aaaaatcaga aaaagcgcgg
     1381 tgccgcgcaa gctgtgaacc gaaatatcgg gcgcgtagga aaaagcgcag gtcaacaccg
     1441 accagccgaa aaacgtccag agcgccaggt cgagcggcgt gcggataaat ttggggcgcg
     1501 gcttcaggaa aagacgaatc agccacgcca gcattcccag aatccacgaa gtctgcgtcg
     1561 cggcaatcga atgcggcgcg gacaaaacca tcaaaacgag aaaaacgaag gcgatgcgct
     1621 caagccatcc ggcaagccgg ttttcgtgat ttactcgcgc gatttcgtca agccatcgaa
     1681 gaaatttttg cataaattaa cacgatagat tttgctttat cctgccattc ctgtctatcc
     1741 tgtctgagat aaacttttaa aatatgagtc aggaaaatct gcgcgacgat ttatcaatca
     1801 aaaccgaaat caatctttat tacgatatgt tcgttcccga aacggcgaaa gggcgcgcgc
     1861 ctctgctcgt cgcggttcac ggctacggcg cgcacaaacg ctatatgatg cgcgaggcgc
     1921 gagccgttgc gccggacggt tttgcgattg tttcgctgca ggcgccgcac cagcattttc
     1981 gcccgaccga tagcggctac aaagtcggtt tcggctggct gaccgattac aaggcgcagg
     2041 aatccgtgcg gctgcatcac aatttcgtgc tcggtgtgat tgaaagattg tcgggcgaag
     2101 atttgattga cccgaaccgg atttatcttt acggtttttc gcaagcctgc gcgctgaatt
     2161 ttcgtttcgc ttttacgttt ccggacgttg cgcgcggcgt gatcggcatt tccggcggaa
     2221 ttcccggcga tttggaaacc agcgaaatat accagccgac taaagcggac gttttttatc
     2281 tttataacga cgacgacgag tttt
//
LOCUS       DASWNE010000927         3339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_278685, whole genome shotgun sequence.
ACCESSION   DASWNE010000927 DASWNE010000000
VERSION     DASWNE010000927.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278685"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="VGB68_18095"
     CDS             complement(<1..1089)
                     /locus_tag="VGB68_18095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051212.1"
                     /translation="MRRLNSTSDLFNGLIFYKRFITLVIIFSLSGSLFAQTRRAGSNT
                     ANNTKPTAQKCSGVWTGSVKYTLTQTHNESKTEPRFLGTVGEDTKRTELKYEYTAKVV
                     VAENAGKKRAKAVIDSKLTGTETTDAREKVSCDRGKTWKTQSGNFVRVTKVYGKSDDA
                     DANVTVGVNADGTYNVAVGLPQIRGESEASYTSTFSGQCREKKGENEKTPPTETSIQG
                     NSLISDGSHRIDPKNPNRLSGSYTRKGIAGMEETISWKLQRCGSPLMITEVELFQPVH
                     PSINNWVKIEGGNYTIDGNFVKVVAKIANVSGETKTTPIEFRELKENTLLPGGKESIT
                     VAPNEEKEIVYMWDTSGYAWKESGELNQP"
     gene            1260..1835
                     /locus_tag="VGB68_18100"
     CDS             1260..1835
                     /locus_tag="VGB68_18100"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0051213.1"
                     /translation="MNDNAPNDVTQMLFQLSSGDSAVLDELLPRIYAELRGLASNYLS
                     REYRKNHTLQPTALVHEAYLRLVNQREARWESRAHFFGAAANVMRQILVDHARRHTAE
                     KRGGELQKMQLEESIVIGTSGKSFGMLALDEALEELEKFDSQKSKIVELRYFGGLSVA
                     ETAAVLGVSEITVKRHWRVAKAWLYDKLKAE"
     gene            complement(1942..>3339)
                     /locus_tag="VGB68_18105"
     CDS             complement(1942..>3339)
                     /locus_tag="VGB68_18105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051214.1"
                     /translation="ADVKIKTRSTSGGASTESTTFIKGKRQRTEMGEQSVSIMQCDLR
                     RSVQLSPAAKTYLVSLFNQTGAAQSIVTKTDGQTETTFVRGGTVTTIVTTKDTGERKQ
                     MFGYAARRIITTMETEPSPDACSKDKTKMEMDDWVIDAEFALVCSEINQSGSVRSYGG
                     SCQDKQVVKRIGAAKSGYPLYSKITMFDENGKPGSSSVREVIELSKENLDAALFDVPG
                     DYREVKNSSELYAANYSSDESKNSSVKTSQMTVANVSSGSKSSADNSSGDVRNAPNNS
                     ADGAKIPGAKKAGVVRLGLAGVKTGSVGEGVNAQELSAAVQNTLAEYLKMPNVEIVRL
                     EAKLPSAIDAEAQEKECDFVIYANVSHKKGGGGMFGKALGKLSETVAGRAYGSADHVG
                     KATQITIMTAAEASGNVKAKDQLTLEIKLVAAGSQSPVAAKQFQAKAKSDGEDIISRL
                     VEQAAQMIVDASAKK"
BASE COUNT          705 a          897 c          850 g          887 t
ORIGIN      
        1 aggctgattc aattcgcccg attccttcca cgcgtacccg gaagtgtccc acatatagac
       61 gatttccttt tcctcgttcg gcgcgacggt gatcgactct ttaccgcccg gcaaaagcgt
      121 attttccttc aattcccgaa attctatcgg ggtggtcttc gtctcgcccg aaacattggc
      181 aatcttcgcc acgactttga cgaaatttcc gtcgatcgta taattgccgc cctcgatttt
      241 cacccagtta ttgatgctcg gatgaaccgg ctgaaaaagt tcgacttccg taatcatcag
      301 cggcgagccg cagcgctgca gcttccagct gatggtttct tccattccgg cgattccttt
      361 ccgcgtatag ctgcccgaaa gccggttcgg atttttgggg tcgattcggt gcgagccgtc
      421 gctgatcaaa gaatttccct gaatcgaggt ttcggtcggc ggcgtctttt cattctcccc
      481 ttttttctcc cggcattgtc cgctgaaagt cgaggtatac gacgcttcgc tttctccgcg
      541 aatctggggc aagccgaccg ccacattata ggttccgtcg gcattcacgc cgaccgtgac
      601 gttggcatcg gcatcgtcgc ttttaccgta aaccttggtg acgcgaacaa aatttccgct
      661 ttgagtcttc cacgttttgc cccggtcgca ggaaactttc tccctggcat cggtcgtttc
      721 cgtgccggtc agcttcgaat cgatcaccgc cttcgcccgt tttttgcccg cattttccgc
      781 gaccacgact ttcgccgtat attcgtattt caactcggtt cttttggtat cttcgcccac
      841 cgtccccaaa aaacgcggct cggtcttgct ctcgttatgc gtctgggtca gcgtgtattt
      901 gaccgaaccg gtccagacgc ccgaacattt ttgggcggtc ggtttagtat tgtttgccgt
      961 atttgacccc gcccgtctgg tctgggcgaa taatgaacct gaaagactaa aaataataac
     1021 taaagttatg aatcgtttgt aaaagataag tccgttgaat aaatcggatg ttgaattcag
     1081 tcgtcgcatc gtgaatctcc ttaaatcgtg ttcgtgccgg aatgccggct tcaatctaag
     1141 tgcgcaaaac gggcgataaa aggttcatac ttaaaataat aaattgtgaa attgcgaaaa
     1201 aagaggtaat tttaagacgc aagaccgata aacaaaataa acatgtattc tttgctgtaa
     1261 tgaacgacaa cgcaccgaac gacgtcaccc agatgctttt ccagctgagc agcggcgaca
     1321 gcgccgtgct ggacgaactg ctgccgcgca tctacgccga gctgcgcggt ctggcgagca
     1381 attatctgag ccgcgaatat cgtaaaaacc atactcttca gccgacggcg ctcgttcacg
     1441 aagcttatct ccggctggtc aatcagcgcg aggcgcgctg ggaaagccga gcgcattttt
     1501 tcggcgcggc ggcaaacgtg atgcggcaga ttctggtcga ccacgcgcgg cgtcacacgg
     1561 ctgaaaagcg cggcggcgag cttcaaaaaa tgcaattgga agaatccatc gtcatcggga
     1621 caagcggaaa atcttttgga atgctcgcgc ttgacgaagc gctcgaagaa ttggaaaaat
     1681 tcgattcgca aaaatcgaaa atcgtcgagc tcaggtattt cggcggcttg tcggtcgcgg
     1741 aaaccgccgc cgttttagga gtgtcggaaa ttacggtcaa acgccactgg cgcgtggcga
     1801 aagcctggct ctacgataaa ttaaaagccg agtaatcatt cataaaattt aaccggctgg
     1861 caatcgaaag gaaactaaaa agtatcaaca aaaaggcggt caatattgat cgcctttttg
     1921 tttggaattt gaaacggcgg tttatttttt cgccgaagcg tcaacgatca tctgcgcggc
     1981 ttgttcgacg agccgggaga tgatgtcttc gccgtcggat ttggctttcg cctgaaattg
     2041 tttcgcggcg accggcgact ggctgcccgc ggcgaccagt ttgatttcga gcgtgagctg
     2101 gtctttcgcc ttaacgtttc cggaggcttc ggcggcggtc ataatcgtaa tttgcgtggc
     2161 ttttccgacg tggtcggcgc tgccgtaagc tctgcccgca acggtttcgc tcaatttacc
     2221 gagcgccttg ccgaacattc cgcctccgcc ttttttgtgc gatacattcg cgtaaatcac
     2281 gaaatcgcat tctttttcct gagcttcggc gtcgatggcg gacggcagtt ttgcttcgag
     2341 ccggacgatt tcgacgttcg gcatttttaa atattcggcg agcgtgttct gaacggcggc
     2401 ggataattcc tgagcattga cgccttcgcc gacgctgccg gttttcacgc cagccaagcc
     2461 caatcgaacg acgcccgctt ttttcgcgcc gggaattttt gcgccgtcgg ctgaattgtt
     2521 cggcgcgttt ctaacgtccc cgcttgaatt atccgcagac gatttactgc cggaagaaac
     2581 gtttgcgacc gtcatttgcg aagttttaac cgagctgttt ttcgattcgt cgctcgaata
     2641 gttcgccgcg tacaattccg aagagttttt aacttcgcgg taatcgccgg gaacgtcgaa
     2701 caaggcggcg tctaaatttt ccttcgacaa ttcgatgact tctctgaccg aagaagaacc
     2761 gggcttgccg ttttcgtcga acatcgtgat tttcgaataa agcgggtaac cggattttgc
     2821 cgcgccgatt cgtttaacga cctgcttgtc ctgacagctg ccgccgtatg accggacgga
     2881 gccgctttga tttatctcgc tgcaaacgag agcgaattcg gcgtcgatta cccaatcgtc
     2941 catttccatt ttcgttttat ctttcgaaca ggcgtccggc gacggctcgg tttccatcgt
     3001 cgtgatgatg cgccgcgccg cgtagccgaa catctgtttg cgctcgcccg tgtcttttgt
     3061 ggtaacaatt gtcgtaaccg tgccgccgcg gacgaaggtc gtttcggttt gcccgtctgt
     3121 tttggtaacg attgactgcg ccgcgcccgt ttgattaaac aaacttacaa gataagtttt
     3181 cgccgccgga gaaagctgaa cgctgcgccg caaatcgcac tgcataatcg aaaccgactg
     3241 ctcgcccatt tcggtacgct ggcgtttgcc tttgataaaa gtcgtgcttt ccgtcgacgc
     3301 gccgcccgac gtgctgcgcg ttttgatttt gacatcggc
//
LOCUS       DASWNE010000928         1580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_666808, whole genome shotgun sequence.
ACCESSION   DASWNE010000928 DASWNE010000000
VERSION     DASWNE010000928.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666808"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VGB68_18110"
     CDS             <1..300
                     /locus_tag="VGB68_18110"
                     /inference="COORDINATES: protein motif:HMM:NF024704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YARHG domain-containing protein"
                     /protein_id="HEY0051215.1"
                     /translation="LIAGYEAKLREKLSTEVLTEDTLGGLFTEDLRILRNEIYARRGR
                     VFKDRELQKYFETTDWYQPNPDFKDDMLGEIEFKNLAAIKLAEENALSKFSEEEG"
     gene            452..1267
                     /locus_tag="VGB68_18115"
     CDS             452..1267
                     /locus_tag="VGB68_18115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEY0051216.1"
                     /translation="MNVVSPEEFELEKKRRKLERLKDRLADREEEMTQLRAELEQTEA
                     NYTMEVGRFYAELDEIEAQIAEEELKLVPDDEEIKKKVEELRRRAEESAADLAEANQN
                     CHNKFNPTPEAKKAYHNLARLIHPDLAIDDEERTRRHGLMARLNDAYAEGDQNLLNKL
                     VEEYRDSPDLVRGNSVGDELVRVIRQISQVAVRLRELKSEKLKAELSEAYAMREKVRA
                     EQSEGRNYFRQMSERTKTHIMKARRRLENLKNLNVAQEDYIQEKYGMDISEFR"
BASE COUNT          456 a          353 c          417 g          354 t
ORIGIN      
        1 ctgattgctg gatatgaagc gaaattacgc gaaaagcttt cgaccgaggt tttgactgaa
       61 gacactctcg gcggactttt caccgaggat ttgcgcattt tgcgaaacga gatttacgcc
      121 cgtcgcggtc gcgttttcaa agacagggaa ttgcagaaat attttgaaac caccgattgg
      181 tatcagccga atcccgattt caaagatgat atgctcggcg aaatcgagtt taaaaatctg
      241 gcggcaatca agctcgccga agaaaatgcg ttgagcaagt tcagcgaaga ggaaggctga
      301 taaagccaaa attcaagttc cgcgtgccgg agctataaaa agcaaaaaga cagacaggat
      361 aaagttttta ttccgtctgt ctttttattt cggtcgcggc tttgccgatg cgagctttgt
      421 cggtttatct taatgcggtt aaaatttcaa tatgaacgtc gtatcgcccg aagaattcga
      481 acttgaaaaa aagcgccgca aattggagcg tctgaaagac cggctggctg accgcgaaga
      541 ggaaatgacg cagctgcgcg ccgagcttga gcagaccgaa gcgaattaca cgatggaagt
      601 cgggcggttt tacgccgagc tcgacgaaat cgaagcgcag attgccgaag aggaattaaa
      661 gctcgtgccg gacgacgagg agataaaaaa gaaagtcgag gagctgcgcc gccgcgccga
      721 agaatccgct gccgacctgg cggaagcgaa tcagaattgt cacaataaat tcaacccgac
      781 gccggaagcg aaaaaggctt atcacaatct ggcgcgcctg attcatcccg atttggcgat
      841 tgacgacgaa gagcggacgc ggcgtcacgg cttgatggca aggctcaacg acgcttacgc
      901 cgaaggcgac cagaatcttt tgaataagct ggtcgaagaa tatcgcgatt cgcccgatct
      961 ggtgcgcggc aattccgtcg gcgacgagct ggtgcgcgtt attcgccaga tttctcaggt
     1021 cgccgtgcgc ctgcgcgagc tgaaaagcga aaaactgaaa gccgaattgt cggaagctta
     1081 cgcgatgcgc gaaaaggttc gcgccgaaca atcggaaggc agaaattatt tccgccaaat
     1141 gtccgagcgc acgaaaactc acattatgaa agcccgccgc cgcttggaga atctgaagaa
     1201 tctgaacgtc gcgcaggaag attatattca ggaaaaatac ggaatggaca tctcggagtt
     1261 tcgttaaaaa aaattaaccg cgaataaaca cggaggaatt tacccgggat gacggcgatt
     1321 gtcatcccaa gtaaattcct ttttcttatg cgcgtcgggc tgcctttgtc tgcggtttcg
     1381 ctttttaaaa atttccgtgt tccggtcaat ttcgcggttc atttctctcg gcaaaatcac
     1441 gtttttaaat gcgtccaata atcataaaat ttatggtttt aaattaataa agagaggaat
     1501 tatatggaca gcagatttgc gcagacgatt attaacattg ccaaagagga agcgacgccc
     1561 gagccgtacg ggaaggttaa
//
LOCUS       DASWNE010000929         5728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_314810, whole genome shotgun sequence.
ACCESSION   DASWNE010000929 DASWNE010000000
VERSION     DASWNE010000929.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5728
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314810"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VGB68_18120"
     CDS             <1..655
                     /locus_tag="VGB68_18120"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0051217.1"
                     /translation="MKKLFSLIVLLLALSPVNSFGQDKSAGAATQQKASVPVSYGIVV
                     DNSGSYRAILERIIEITKDIVEANRADDETFLMRFVSTDKIKLLQDFTVSKDEIHAAA
                     DEMFVEGGLTAILDAVDFAARHLAEKASGEASRRKVLILLTDGDDRRNKAKIEEVLKF
                     LKDNQIQVFVVGISEEKVSGKIIDKLTRETGGRKFVPKTPAEVAATIKELSAAIRAQ"
     gene            678..1148
                     /locus_tag="VGB68_18125"
     CDS             678..1148
                     /locus_tag="VGB68_18125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY0051218.1"
                     /translation="MAENTVGNRVCSTCGADVRPQALFCYSCGSSVAPEIENSERVEA
                     TDNARFQEEIVEPDIAAAKVTTNLNENHGTPIEKPFDTLVEDKTEKKSPLQAETKLKS
                     AAALRRGARAPAQKKTVEVIWEEPENAPNIWFLLAGFVLALFAVGILLAMLYIR"
     gene            1229..1549
                     /locus_tag="VGB68_18130"
     CDS             1229..1549
                     /locus_tag="VGB68_18130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HEY0051219.1"
                     /translation="MEALGMVECMGLVAMIEAADAMVKAANVRLVGYEKVDAGLVTAI
                     VRGEVGAVRAAVDAGAAAARRVGTVTAVHIIPRPHAEVADGIPVIETGETTRKKISGS
                     VPEK"
     gene            complement(1771..2907)
                     /locus_tag="VGB68_18135"
     CDS             complement(1771..2907)
                     /locus_tag="VGB68_18135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0051220.1"
                     /translation="MKRKSTNIFLLVSGLILTAAFGFFTYPKAQAQTSNTRPTAAPTK
                     APTVKPTASPVESPTPDIDDPNEIIKVKTELVNLNVRIIDRNNRPISNVQKREVKIYE
                     DNVLQEIDNFSRAEVPTNYALVIDNSGSLRHQLDKVIDASKVIVSTNRQDDETSVVRF
                     VSRDKISIEQPFTPNKADVMEALDNLFIEGGQTAVIDAVYLAAEAVDQYEKTRNPNDK
                     KRRALILVSDGEDRDSYYNEQQLFELLREADVQIYAIGFTTDLSKEGGFITKSPKAKS
                     EAFLRRLAEETGGKAYFPQAISELDTIAKDIAAEMRTQYIISYYPTNEEADSRFRNIK
                     VVVDDGPNKQKRIAVTRSGRTAAGDDPNAKPVLQTTTQSKPNNQ"
     gene            complement(2933..3922)
                     /locus_tag="VGB68_18140"
     CDS             complement(2933..3922)
                     /locus_tag="VGB68_18140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0051221.1"
                     /translation="MAVNDFVGISRLRGFPFRIFLCAALVFSAATAFAQTPQRTPPAG
                     DDDVIKVPTDLVTLTLTVTDQYGRYVSGLNKNAFTIFDNNQEQEITFFSDTDAPISIG
                     ILFDVSGSMSGDKIARARKALERFINTSHPMDEYFLIAFNTRAQLLLDRTRNGEAVLD
                     KLTLVQPKHNTALYDACYLGIERVTRGSHQKKAMLIISDGQDNSSRYNFGEVRRLMKE
                     ADVTVYAVGIVDGADSSSALGMQGQAFLDELAGVTGGKSFYPQTNVEMDEIFERIALE
                     LRHQYSIGYTPKDFTLDGKWRKVKVKVKPPRGLPRLIVRSREGYYATPNTPDN"
     gene            4245..5480
                     /locus_tag="VGB68_18145"
     CDS             4245..5480
                     /locus_tag="VGB68_18145"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY0051222.1"
                     /translation="MAEFICRLGTPSGEIITRIIEATAPGEAKTRLESEGFRVFAVSN
                     AERGLSAVLPMGANKKSRVKSGDFLLFNQQLSALLRAGIPVLQSINLLKSRSQSSAMR
                     TVLMDVEEKIKSGIPLSEAFEAQGIFPKIYTASLLAGERSGALDDVLARYVDYLKRSV
                     GVSRKLRGALAYPAFLLLAASFMVMFLTIYVVPRMSDLFKGLSANKGLPTITLVVMAI
                     SNFVSGNFWWLLPLILIFIFATYLWLRTPSGKLVLHAFLLRLPLAGNLIKQMTTAQLA
                     RSISTLLAGGITVPDSWDIASEAITNLELRRRSQAVLPMIREGRGFTEALDKANWMPE
                     LALDMIGIGEKSGSLREMLDEVANFYDAETEVRLEQLTTLLEPIILVVMAGVVITILL
                     AIYMPIIQSISSGPFGGRK"
     gene            5537..>5728
                     /locus_tag="VGB68_18150"
     CDS             5537..>5728
                     /locus_tag="VGB68_18150"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEY0051223.1"
                     /translation="MKVWEKSHQLTLLAYQATKNFPREELFGLTSQIRRACSSIPANI
                     AEGCGRQTERDFPRFLQIAF"
BASE COUNT         1343 a         1384 c         1584 g         1417 t
ORIGIN      
        1 aatgaaaaaa cttttttcgt taatcgtcct gcttctggct ttatcgccgg taaattcttt
       61 cgggcaggac aaaagcgcgg gtgcggcgac gcagcagaaa gcgtccgttc cggtcagcta
      121 cggcatcgtt gtggataatt ccggctcgta ccgcgcgatt ctggaacgga ttatcgagat
      181 aacaaaagat attgtcgagg caaacagggc ggatgacgag acgtttctga tgcgcttcgt
      241 cagcaccgat aaaatcaagc tgctgcagga ttttacggtc agcaaggacg agatacacgc
      301 ggcagctgac gagatgtttg tcgaaggcgg gctgacggca attctcgacg cggtcgattt
      361 cgccgcccgt catctcgccg aaaaagcgag cggtgaagcc tcgcggcgaa aagttttgat
      421 tctgctgacc gacggcgacg accgccggaa caaagcgaaa atcgaagaag ttttgaagtt
      481 tttaaaagac aatcagattc aggtttttgt cgtcggcatc tcggaagaaa aagtttcggg
      541 aaaaattatc gacaaactga ccagagaaac cggcggcaga aaatttgttc cgaaaacgcc
      601 ggcggaagtt gcggcgacga taaaagagct gtccgccgcg attcgcgctc aataagtatt
      661 atttaatctg catgataatg gctgaaaata cggtaggaaa tcgagtttgt tcgacttgcg
      721 gcgcggatgt ccgcccgcag gcgctttttt gctacagttg cggctcgtcg gttgcgcccg
      781 aaatcgaaaa cagcgaacgc gtcgaagcga cggacaatgc gcgatttcaa gaagagattg
      841 tcgagccgga catcgccgca gcgaaagtga ccaccaatct gaatgaaaac cacggtacac
      901 cgattgaaaa gccgttcgat acattggttg aagataaaac ggaaaaaaaa tctccgcttc
      961 aggcggaaac aaaattaaaa tccgccgccg ctctccggcg cggcgcgcga gctccggcgc
     1021 agaaaaaaac cgtcgaggtc atctgggaag agccggaaaa cgcgccgaac atatggtttc
     1081 tcctcgccgg ttttgtttta gcgcttttcg ctgtcggaat tttgctggcg atgttgtata
     1141 ttagataaaa attacaaggg cgcgccgtcg ccgcttttaa cgcagattcg gttggaaagg
     1201 caaacgaaaa tttcgtttta aaaagtttat ggaagcactc ggaatggtcg aatgtatggg
     1261 cttggtggcg atgattgaag ccgccgatgc gatggtcaaa gcggcaaacg tccgtctggt
     1321 cggctacgaa aaagtcgatg ccggactggt gacggcaatc gtgcgcggcg aagtcggcgc
     1381 ggtacgggcg gcggtcgacg ccggtgcggc ggcggcgcgg cgggtcggca cggtgacggc
     1441 ggttcacatc attccgcgcc cgcacgccga ggtcgccgac gggattccgg ttatcgagac
     1501 cggcgagacg acgcgcaaaa agatttccgg ctcggttccc gaaaaataaa tacaaaaaat
     1561 aaatgcagaa gcatcgcgtt tcaatcaagg cttatttttc gggttgataa aacagacggt
     1621 gccggagcag ccgagcgatt aattggaatt gcccgaaaat gccgggaaaa ttaattgacc
     1681 taaagtaaaa aaaaataatc gtcttcagat agaaaaagcg gattttccga attggagaat
     1741 ccgcttttta aattattcga gagcgaaagg ttattgatta ttcggtttcg attgcgtcgt
     1801 cgtttgcaaa accggtttcg cgttcgggtc gtcgccggcg gcggttctgc cggagcgtgt
     1861 aacggcgatt cgtttttgtt tgttcggtcc gtcgtcaacc acgaccttga tgtttcggaa
     1921 acgactgtcg gcttcctcgt ttgtcgggta atagctgatg atgtactgcg ttctcatttc
     1981 cgccgcgatg tctttggcga tggtgtccag ctcgctgatt gcctgcggaa aataagcctt
     2041 gccgccggtt tcttccgcca gccggcgtaa aaacgcttct gatttcgctt tcgggctttt
     2101 ggtaataaaa ccgccttctt tgctcaggtc ggtcgtaaat ccgatggcgt aaatctgcac
     2161 gtccgcttcg cgcagaagct cgaaaagctg ctgctcgtta taatagctgt cgcggtcttc
     2221 gccgtcggag accagaatca gggcgcggcg ttttttgtcg ttcgggttgc gcgttttttc
     2281 atattggtcg accgcttccg ccgccagata aaccgcgtcg ataacggcgg tttgcccgcc
     2341 ttcgataaaa agattgtcga gcgcctccat cacgtcggct ttgtttggcg taaaaggctg
     2401 ctcgatgctg attttatcgc ggctgacaaa gcgcacgacg cttgtttcat cgtcctgacg
     2461 attggtgctg acgataacct tgctggcgtc gatgacttta tcgagctgat gacgcagcga
     2521 gccggagtta tcaatcacca gcgcgtaatt ggtcggaact tccgcccttg aaaaattgtc
     2581 gatctcctgc aaaacgttat cttcgtaaat cttgacttcg cgtttctgga cgttgcttat
     2641 cgggcgattg ttgcggtcga taatgcggac gttgaggttg acgagctcag ttttaacctt
     2701 gatgatttcg ttcgggtcgt cgatgtcggg cgtcggcgat tcgacgggcg aagctgtcgg
     2761 tttgaccgtc ggcgctttgg tcggcgcggc ggtcgggcgc gtgtttgagg tttgcgcctg
     2821 cgctttcggg taagtaaaaa agccgaaagc cgctgttaaa atcaaaccgg agacaagcag
     2881 aaaaatattc gtcgattttc ttttcattta atttccaatc cgtagctcta aactaattgt
     2941 caggcgtatt cggcgtcgcg taatagcctt cacgactgcg gactattaga cgcggcaaac
     3001 cgcgcggagg tttgacctta acttttactt tccgccattt tccgtcgagc gtgaaatctt
     3061 tcggcgtgta gccgattgaa tattgatgac gcagctcgag cgcgatacgc tcaaaaattt
     3121 cgtccatttc gacgttggtt tgcggataga aagattttcc gccggtcacg cccgccagct
     3181 cgtccagaaa agcctgaccc tgcattccga gcgcgctcga agaatcagcg ccgtcgacga
     3241 ttccgacggc gtagacggtg acgtccgctt ctttcatcag gcggcgcacc tcgccgaaat
     3301 tatagcgcga gctgttgtcc tgaccgtcgc tgataatcag catcgctttt ttctggtgcg
     3361 agccgcgcgt gacgcgctcg atgcccagat agcacgcgtc gtaaagcgcc gtgttgtgtt
     3421 tcggctggac gagcgtcagc ttgtctaaaa ccgcttcgcc gttgcgcgtc cggtcgagca
     3481 gaagctgcgc gcgcgtgtta aaggcgatca gaaaatattc gtccatcggg tgactcgtgt
     3541 tgataaagcg ttcgagcgcc ttgcgcgccc gcgcgatttt atcgccgctc atcgagccgg
     3601 agacgtcaaa caggatgccg atggaaatcg gcgcgtccgt gtcgctgaaa aacgtgattt
     3661 cctgttcctg gttgttgtcg aaaatggtaa aggcgttttt gtttaagccg gagacgtagc
     3721 gcccgtactg gtcggtgacg gttaaggtca gcgttaccaa atcggtcgga acttttatta
     3781 cgtcgtcgtc acccgcgggc ggagttcgtt gaggcgtctg agcgaaagcc gtggcggcgg
     3841 aaaaaaccag cgcggcacac agaaaaatcc ggaacggaaa accgcgcaaa cggctgattc
     3901 ctacaaaatc atttacagcc ataaaatctc ctctaattta aaattcattt taggtgacgt
     3961 ccgctaaaca aagcaagata aatttttgat gtggctaaaa gaaatttcgt tgttccgcgc
     4021 caagaaaagt ttgcaacctt ttttcggaaa aaagcgtgat atactcgaca taatttgcga
     4081 ttttagattg acgattggcg atttgtcgag gaaaattaga tttttccctg aaattcgcgg
     4141 cgaactttta cctttttgac tcatcttata aaacttaaaa taatttttgc gaagcaaggt
     4201 cttcgcccaa tcgcaaatcg ctaaacgcgc aaatcgaatt taatatggct gaatttatct
     4261 gtcgtctggg aacgccttcg ggcgaaatta taacgcgcat tatcgaagcg accgcgccgg
     4321 gcgaggcgaa aacgcggctc gaaagcgagg gctttcgcgt tttcgccgtc tcgaacgccg
     4381 aaagaggttt atccgccgtt ttgccgatgg gcgcgaacaa aaaatcgcgc gtcaaatcgg
     4441 gcgattttct gcttttcaat caacagcttt cggctctgct gcgagcggga attcccgttt
     4501 tgcaatcgat taacctgctg aaatcgcgct cgcagtcttc ggcgatgcgc acggttttga
     4561 tggacgtcga agaaaaaatt aaaagcggta ttccgctgtc cgaagccttc gaagcgcagg
     4621 gaatttttcc gaaaatctac acggcgtccc ttttggcggg cgagcgcagc ggcgcgctgg
     4681 acgacgttct ggcgcgttac gtcgattatc tgaaacgaag cgtcggcgtc tcgcgcaaac
     4741 ttcgcggcgc gcttgcctat ccggcgtttc ttttgctggc ggcgtcgttt atggttatgt
     4801 ttctgacgat ttacgtcgtg ccgcgaatgt cggatttgtt taagggactc agcgccaaca
     4861 aaggtttgcc gacgataacg ctggtcgtga tggcaatctc gaattttgtt tcgggcaatt
     4921 tctggtggct gctgcctttg attttgattt ttattttcgc gacgtatctc tggcttcgga
     4981 cgccgagcgg aaaactcgtt ttgcacgcgt ttctgcttcg cctgccgctg gcgggaaacc
     5041 tgataaagca aatgacgacg gcgcagctgg cgcgctcgat ttcgactctg ctggcgggcg
     5101 gcatcacggt tcccgattcg tgggacatcg cctcggaagc gattacgaat ctggagctgc
     5161 gccgccgcag tcaagccgtt ctgccgatga tacgcgaggg cagaggtttc accgaagctc
     5221 tggacaaagc gaactggatg ccggaactgg cgctcgatat gatcggcatc ggcgaaaaat
     5281 cgggcagcct gcgcgagatg ctcgacgaag tggcaaactt ttacgacgcg gaaaccgagg
     5341 tgcggctgga acaattgacg acgctgctcg aaccgataat tctggtcgtc atggcgggcg
     5401 tcgtcatcac gattctgctg gcgatttata tgccgattat tcaatcaatc tcgtcgggac
     5461 cgttcggcgg cagaaaataa gcggtcagcc gtcagctatc agccgtcagc tttaagaaat
     5521 gagaaatttt agagaattga aagtctggga aaaatctcat caactgacgc tgctggctta
     5581 tcaagccaca aaaaattttc ccagagagga attatttgga ttaacgagtc aaattcgccg
     5641 tgcttgttct tctattcccg caaatattgc cgaaggctgc ggtagacaaa ccgaaagaga
     5701 tttcccaaga tttttacaga tagcattc
//
LOCUS       DASWNE010000930         4409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_170573, whole genome shotgun sequence.
ACCESSION   DASWNE010000930 DASWNE010000000
VERSION     DASWNE010000930.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170573"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VGB68_18155"
     CDS             complement(<1..371)
                     /locus_tag="VGB68_18155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051224.1"
                     /translation="MFAAQNGWQSISNADVNFFQNSNYYRLFAAGRNKNIAGLMHRQY
                     GGGQVFLFDYEYTTGSGKSTKVHTQTVVAYHSPHLNLPYFSLYPESFLSFIGEAFGYN
                     DIDFASHPHFSKRYKLSGGDE"
     gene            complement(631..1656)
                     /locus_tag="VGB68_18160"
     CDS             complement(631..1656)
                     /locus_tag="VGB68_18160"
                     /inference="COORDINATES: protein motif:HMM:NF021537.4"
                     /note="predicted Zn-dependent protease with a strongly
                     conserved HExxH motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing putative Zn-dependent
                     protease"
                     /protein_id="HEY0051225.1"
                     /translation="MKKLIAIALCLVICALGIFAQTAKVNDDPKKAKFVTSDIDNFWR
                     AFDLAAKETDRERKIAIYQAEYLDKGSVGLKDFVRLRIKSAKDLTETVESLPRFYASV
                     RASSLRVKEMEKQMRKAFVKFKKIYPEAVFPDVYFVIGVTNTGGTASNNGLLVGTELY
                     GLTGQTPRDEFFEAFRKRAPKDLDEKQLRERAERLLNIMLKPIENVTPLVAHESCHFN
                     QKYAALDTLLAKSVQEGACDFIAEKTAGETLNIQQKIYGDRHETELWKEFEAQMSGTD
                     YKNWMYNGITSGARPPDLGYYMGYKISHAYYRNARDKRQAIKDILETKDFKAFLEKSR
                     YRQKFSE"
     gene            complement(1821..2606)
                     /locus_tag="VGB68_18165"
     CDS             complement(1821..2606)
                     /locus_tag="VGB68_18165"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEY0051226.1"
                     /translation="MEWLSDPQLWIAFATLTVLELVLGIDNVIFISILSGKLPPAQQS
                     KARYIGLALALIMRVILLFSLSWVIGLTAPLFSIFGRDISGKDLVLLVGGLFLIAKST
                     HEIHGSLEGEEGHGSKKSYASFSGVIIQIVLLDIVFSLDSVITAVGMVSTEFGDAGIW
                     IMIGAVVISIAAMMLFAGPIGAFVQRHPTVKMLALSFLLLIGVMLVAEGFHQKIPKGY
                     IYFAMAFSVIVEILNMRLRKKADPVHLHNAYADDDEAKAIASS"
     gene            complement(2775..3170)
                     /locus_tag="VGB68_18170"
     CDS             complement(2775..3170)
                     /locus_tag="VGB68_18170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051227.1"
                     /translation="MFRKCALMAVFLTALGLLAPANQVAAQARARVRFARGASSATFR
                     GRINGYRYVDYIVGARAGQTLRLKLQTTGSAEIVVFTPPDENIDGADGLEYTGEIQYT
                     GDYKIRVLQPRAFARRNNASNYTLRISIE"
     gene            complement(3234..>4409)
                     /locus_tag="VGB68_18175"
     CDS             complement(3234..>4409)
                     /locus_tag="VGB68_18175"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY0051228.1"
                     /translation="ALIGHNSVRAKVMGLDNRAPSAEEQSKMEQLVEQAMKDGALGLS
                     TGLIYLPGTFAKTDEVIGLAKVASRYGGIYSSHIRDEGTEVVKAIEEAINIGEQANMP
                     IEISHFKISSKALWGNTPTTIGLVKSARERGLSVTVDQYVYTASSTSLTARMPAWAVA
                     GGLAEGKKRLADAATREKIVREMKEELKKKDFKDYSFAYVASYAPNPEFNGKNIAEIT
                     KMTRGKKKLDAQIEQIFEMYEKGGASMVYRVMSEEDVENILREPFTMIASDSSVRNFG
                     AGVPHPRGYGNNARVLSEYVRARKIISLEDAIRKMTSLPAQTFGLRERGQIREGMAAD
                     LVIFDENKIADKATFENPHQYAEGFQAVVVNGEIVFDGQKMTGKMSGAPLYGNGYQR"
BASE COUNT         1065 a         1139 c         1094 g         1111 t
ORIGIN      
        1 ctctcgtctc cgccgctcaa tttgtagcgt ttggaaaaat gcgggtgcga ggcaaaatca
       61 atatcattat agccgaaggc ttcgccgata aaactcaaaa atgattcggg ataaagcgaa
      121 aaataaggca aattcaaatg cggcgaatga taggcgacaa ccgtctgcgt gtggactttt
      181 gtcgattttc cgctgcctgt cgtgtattcg taatcgaaaa gaaaaacttg tccgccgcca
      241 tactgccggt gcatcaagcc ggcaatgttt ttatttctgc cggcggcaaa cagcctgtaa
      301 tagttcgaat tttgaaaaaa atttacgtct gcgttcgaga tagattgcca gccgttttgg
      361 gcggcgaaca tctgtagatt ccggattctc ttttgttttt gcttacggag aaaagctact
      421 aatccgacac tgagcaggaa tataattccc acaaatgaga aaatcagaat ggcaacgata
      481 gttaagcccg ttgccgccgc gccgttatcg cggccggttt gccaaagcag aaggaaatcg
      541 aatagaaaac acggaagctg catgaaataa ttcctcgcct gtttagttga gagttcttaa
      601 ttgaaaatcg ttccgcgcgg tgaaaactgc ttattcggaa aatttttggc gataacggct
      661 tttttccaga aaagctttga aatctttggt ttccagaatg tctttaatcg cctgtcgctt
      721 gtctctggcg tttcggtaat aggcgtggct gattttatag cccatataat aacccaaatc
      781 cggcggtcgc gcgccggatg tgatgccgtt gtacatccag tttttgtagt ccgtgcccga
      841 catctgcgct tcaaattcct tccacaattc ggtttcgtgt ctgtcgccgt agattttctg
      901 ctgtatgttc aaggtttcgc cagccgtttt ttcggcgata aaatcgcacg ctccttcctg
      961 cacggatttt gccagaagcg tgtcgagcgc cgcatatttc tggttaaaat gacaggattc
     1021 gtgcgccacc agcggcgtta cgttttcaat cggcttgagc attatattta aaagcctctc
     1081 ggcgcgctcg cgcaattgct tttcatccaa atcctttggc gcgcgctttc gaaaagcctc
     1141 aaaaaactcg tcgcgcggcg tctgtccggt caaaccgtat aactcggttc cgacgagcaa
     1201 accgttgttc gaagccgttc cgcccgtgtt cgtgacgccg atgacaaaat aaacgtccgg
     1261 gaaaacggct tccgggtaaa ttttcttaaa tttcacgaag gctttccgca tctgcttttc
     1321 catttcctta acgcgcaggc tgctcgccct gaccgaggcg taaaatcgcg gcagactctc
     1381 gaccgtctcc gtcaaatcct tcgcgctctt gattcgtaaa cgcacgaaat ccttcagacc
     1441 gacgctgccc ttgtccagat attccgcctg gtaaatggcg attttccgtt cgcggtcggt
     1501 ttccttcgcc gccaaatcaa acgcgcgcca gaaattatcg atgtccgaag tgacgaactt
     1561 cgcctttttc gggtcgtcgt tgactttcgc cgtttgcgca aaaatgccca aagcgcagat
     1621 gaccaaacac aatgcgattg cgattagctt tttcataact ataggatgtg agcagaatta
     1681 ctttaagcct gtttttcgat tcaatcaaca gaaatttcga aaaatttcgg tcaatcaaaa
     1741 agattatgtc aaaaaatcta aaaacaaaaa gacaggcacg gagcctgtct tttaaattca
     1801 aagcttcgga ttacaaaaag ctagctcgac gcaatcgctt tggcttcgtc gtcgtcggcg
     1861 taagcgttat gaagatgaac cgggtcggct tttttccgca ggcgcatatt taaaatttca
     1921 acgataaccg aaaacgccat cgcaaaatag atgtagccct tcggaatttt ctggtgaaat
     1981 ccttcggcga ccagcatcac gccgattaaa agcagaaagg agagcgcgag catttttacg
     2041 gtcgggtgac gctggacgaa cgcgccgatc ggtccggcaa aaagcatcat cgcggcaatc
     2101 gagataacga ccgccccgat cataatccag atgccggcat ctccgaattc cgtgctgacc
     2161 attccgaccg ccgtaataac ggaatcgagc gagaacacaa tatcaagcag cacgatttga
     2221 atgatgacgc cgctgaacga ggcgtatgat tttttggagc cgtgaccttc ttcgccttcg
     2281 agcgagccgt gaatctcgtg cgtggatttg gcaattaaaa acaatccgcc gaccagtaaa
     2341 actaaatcct tgccggagat gtcgcgtccg aaaatagaaa acagaggcgc tgttaatccg
     2401 ataacccagg aaagcgaaaa cagcagtatg acgcgcatta tcaaggcgag agccaagccg
     2461 atatatcgcg ccttcgactg ctgtgcgggc ggcagcttgc cggaaagaat cgatataaaa
     2521 atgacgttgt cgatgccgag aacgagttcc agaaccgtta aggtcgcaaa agcaatccaa
     2581 agctgcgggt cagatagcca ttccatttag aaagtctcct tgaaaaaatt attgatcaat
     2641 aaattccggc gttttaacac gcggaaaagc ttgatgacaa aaagttaaga tttgaaataa
     2701 aagcgagccg ggagacagga aaactgttgt ttccgcttcc cgatattttg cttgcggcgc
     2761 gcgggcgcgg gatttcattc gatgctgatg cgcagcgtgt aattggaagc gttgttgcgg
     2821 cgggcgaagg cgcgcggttg caggacgcgg attttatagt cgcccgtgta ttggatttcg
     2881 cccgtgtatt cgagtccgtc ggcgccgtcg atattttcgt ccggcggcgt gaagacgacg
     2941 atttccgcgc tgcccgtcgt ctgtagcttg agcctcaacg tctgaccggc gcgggcgccg
     3001 acgatgtaat cgacgtatct atagccgttg atgcgacctc taaacgtcgc ggaagaagcg
     3061 ccgcgggcaa accggacgcg cgcgcgcgct tgcgccgcga cctgattagc cggagcgagc
     3121 aaaccgagag cggttaaaaa taccgccatc aaagcgcatt ttctgaacat cggcttttaa
     3181 attttctccg aaaacaaaat ccgtaacaaa aatccgtcga aaacaaaaag attttatcgc
     3241 tgatagccgt tgccgtaaag cggcgcgccg gacattttgc cggtcatttt ctgcccgtcg
     3301 aaaacgattt cgccgttgac gacgactgcc tgaaagcctt cggcgtattg atgcggattt
     3361 tcaaacgtcg ctttgtcggc aattttgttt tcgtcgaaaa tcaccaaatc cgccgccatt
     3421 ccttcgcgga tttgaccgcg ttcgcgcagc ccgaaagttt gcgccggaag cgaagtcatt
     3481 ttacgaatcg cgtcttccag agaaattatt ttccgggcgc gcacgtattc gctcaaaacg
     3541 cgcgcgttgt tgccgtagcc gcgcggatgc gggacgcccg cgccgaaatt gcgcacgctg
     3601 ctgtcggagg cgatcatcgt aaacggctcg cgcagaatgt tttccacgtc ttcctcgctc
     3661 atcacgcggt aaaccatcga agcgccgcct ttttcataca tctcgaaaat ctgctcaatc
     3721 tgcgcgtcga gctttttttt gccgcgcgtc atttttgtga tttccgcgat gtttttgccg
     3781 ttaaattccg gattcggagc gtaagaggcg acgtacgcga agctgtaatc cttaaaatct
     3841 tttttcttga gctcttcttt catctccctg acgattttct cgcgcgtcgc cgcgtcagcc
     3901 agacgttttt tgccttccgc cagaccgccg gcaaccgccc acgcaggcat acgcgcggtc
     3961 agcgaagtcg aagaggcagt gtaaacatac tggtcgaccg tgacgctcag accgcgctcg
     4021 cgcgccgatt tcaccaagcc gatggtcgtc ggcgtattgc cccagagagc cttgctggaa
     4081 attttaaaat gcgagatttc gattggcata ttcgcctgct cgccgatgtt gatggcttcc
     4141 tcgatggctt tgacgacttc cgttccttcg tcgcggatgt ggctgctgta aattccgccg
     4201 tagcgcgagg cgactttcgc cagcccgatg acttcgtcgg ttttggcaaa cgtgccgggc
     4261 aggtaaatca agccggtcga aagcccgagc gcgccgtctt tcatcgcctg ttcgacgagt
     4321 tgttccattt tcgactgttc ttcggcagac ggggcgcgat tatccaaacc catcactttc
     4381 gcgcgaaccg aattatgacc gatgagcgc
//
LOCUS       DASWNE010000931         1634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_562973, whole genome shotgun sequence.
ACCESSION   DASWNE010000931 DASWNE010000000
VERSION     DASWNE010000931.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562973"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1063)
                     /locus_tag="VGB68_18180"
     CDS             complement(<1..1063)
                     /locus_tag="VGB68_18180"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051229.1"
                     /translation="MRKVTEAICQYLKESFGEAQQTLIVGHDSRFMGEKFTAIAAEIA
                     SRKGFRVLLCENPTPTPTISHAIRKEKAAGGINFTASHNPPEYQGIKFSTADGAPALP
                     EITKRIEEIIEKEPQAADAAGGTIERYDARPAYLDDLKTKIRFDLIAAGKGKFAYDAL
                     WGTGRGYLDKILRDSGLEVETLHDWRDVTFGGRSPEPGASHLDELRETVLKNGCLLGV
                     ATDGDGDRFGVLDSDGTFIEPNQLIAILFDYLAESRGWNLGIARSVATSHLVDRVAES
                     RNLKVYETPVGFKFIGELINKDEIILGGEESAGLSIRGHYPEKDGILACLLAVEAVTV
                     RQKSLTAQLNEIFERVGRLE"
BASE COUNT          410 a          413 c          360 g          451 t
ORIGIN      
        1 cttcgagtcg tccgactctt tcaaatattt cgtttaactg cgccgttaaa cttttctgtc
       61 tgaccgttac cgcttcgacc gctaaaagac acgccagaat tccgtctttt tcgggataat
      121 gcccgcggat tgaaagaccg gcggattctt caccgcccaa aataatctcg tctttgttta
      181 tcaattcgcc gataaactta aagccgaccg gcgtttcgta gactttcaaa tttctgcttt
      241 cggcgacccg gtcaaccagg tgcgatgtcg ctacgctgcg ggcgatgccg agattccagc
      301 cgcgcgattc cgccagataa tcgaataaaa ttgcaatgag ctggttcggc tcgataaatg
      361 tgccgtccga gtccagcacg ccgaatctgt cgccgtcgcc gtcggtggcg actccgagca
      421 ggcaaccgtt tttcaaaacc gtctcgcgaa gctcgtccag atgacttgcg cccggctccg
      481 gcgagcgacc gccgaatgtg acgtcgcgcc agtcgtgaag cgtctcgacc tctaaaccgc
      541 tgtcgcgcag gattttatcc agatagccgc gacccgttcc ccagagcgcg tcgtaggcga
      601 atttgccttt gcccgccgcg attaagtcaa agcgaatttt tgttttcaaa tcgtccagat
      661 aagccggacg cgcatcgtat ctttcaatcg ttccgccggc agcatctgcg gcttgcggct
      721 ctttttcgat aatttcttca atccgcttcg tgatttccgg cagagccggc gcgccgtccg
      781 cggtcgaaaa cttgatgccc tgatattcgg gcggattatg cgaagccgta aaatttattc
      841 cgcctgccgc tttctcttta cggattgcgt gcgagatggt cggcgtcggc gtcggatttt
      901 cacagagcag aactcgaaac ccttttcggc tggcaatctc ggcggcgatg gcggtaaatt
      961 tttcgcccat aaagcgtgaa tcgtgtccga caattaatgt ctgctgcgcc tcgccgaaac
     1021 tttccttcag atactggcag attgcttcag ttactttcct cacattcgca aaggtgaatt
     1081 catcggctat aatggctctc caacctgacg ttccgaagcg aatggtcata attttaccgg
     1141 cgaaagtatt tctgctgctt cccttattag ttcccttggt ttgtactgct attttagtgg
     1201 acttgcctga atcctggaat ttctactagt attaccttga aggtgaaaca aaatttagca
     1261 taacggaaaa ctctttgtaa agaagtttct ttaaagatta agctgtttat tttcaataat
     1321 ttgctaaaac atcctcaagt aaacgttaaa ctgactgctt aaagatgaac aaaaggtgaa
     1381 cgaaaatccg caaataatgc aaagcacgta ttttcctaga taatacgtta aatatttaaa
     1441 taataacttt tccacatctg tgcaaaagtt aagcaaaagt ttaaatcaaa attaaatttc
     1501 atataaaaaa taacggcgag cgcagtatct tttttgtgtc aaaaagctca aaacgaagcg
     1561 ccggcagaga atttgtatca gactgctaca aatcggactc gcgcggctta atctcaaaaa
     1621 gcctgtaagt catt
//
LOCUS       DASWNE010000932         5691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_563000, whole genome shotgun sequence.
ACCESSION   DASWNE010000932 DASWNE010000000
VERSION     DASWNE010000932.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5691
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563000"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VGB68_18185"
     CDS             <1..206
                     /locus_tag="VGB68_18185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051230.1"
                     /translation="SFYLNLQCWLTEKCYADSLNQSYNELQILGKLSPVFVLLTFWWG
                     IAIILSLIALFTNKQSDARQSKK"
     gene            complement(252..1805)
                     /locus_tag="VGB68_18190"
     CDS             complement(252..1805)
                     /locus_tag="VGB68_18190"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY0051231.1"
                     /translation="MAKAEKASEAKSKKTASKTTTKTFYNYIGGEWTKSASGEWFENI
                     NPADTNDVIGRFPLSNEEDVNRAVEAAKAAATKWRRTPAPRRAEILYRLGEILAKNKD
                     RYTRDMTREMGKVLKEAGGDVQEAIDCTYYTAGEGRRLHGFTTPAEMPNKFAMCVRQP
                     VGICGLITPFNFPMAIPSWKLIPALICGNTVVIKSGEDVPLSALNLIKSCEEAGIPKG
                     VVNLVNGHGADVGSPLVNHRDIRLISFTGSTDTGRIIAEQCARDNKIVSLEMGGKNAI
                     IVMDDADIDNAVEGSLWGAFGTSGQRCTASSRLVVHKKVYKKFCEKLVEKAKRLRVGN
                     GLDEKTEVGPVINQAAMEKILGYIEIGQKEDKATLACGGNRLTKGDYKNGYFIEPTVF
                     TDVAPKMRVGQEEIFGPVTSVIPFSTLDEAIDIVNDVKYGLSSAIYTQDVNQSFYAMQ
                     ELYTGICYVNSATIGAEVHLPFGGTKGTGNGHREAGTQVLDIFSEWKSLYIDYSGKLQ
                     KAQIDLVEI"
     gene            complement(1873..3447)
                     /locus_tag="VGB68_18195"
     CDS             complement(1873..3447)
                     /locus_tag="VGB68_18195"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY0051232.1"
                     /translation="MAGYCGEIKEQIWRAKRVWFVVLAAACLLLASGIVLGMLAGGRL
                     ITAETPENLRVAPETLSASFAEVSRRVEPAVVNIDTKGKVPEVTLKGEQQTEPTNPDD
                     ILEYFKRQLPRRPSYSVGSGFIVDKTGYILTNFHVVDDASRISVRLQNGEEYIAKVVG
                     TDDETDLAVLKVEAGKDLPTVKFGDSDAVQVGDWVLAVGSPFGLAQTVTAGIISQTRR
                     ETPYTTSFQRFIQTDAAINRGNSGGPLVNMNGEVIGVNSQIASTTGDYNGIGFALPSK
                     EAAYVYQQILANGKVRRGYLGVNLDSVKPEFAKVYNLGETKGAIVTDIRDRTGAASKA
                     GLQINDVITEFNGEAVVSALDLIAKVAATEPLKEINIAYLRESGDKLERQTTVIKLGE
                     RPSNSPTAEGDTTRKKLGVAPTAAPLAPLGLTLAEITPQLATTYKLEGQSGLVIKNID
                     PASFIADLKATNGADALNAGDLIQRINRTSVTDLKTFSDITEKLKTGDAVVLHVASYN
                     KFTRAVQQRIVQFTVQ"
     gene            complement(3582..4544)
                     /locus_tag="VGB68_18200"
     CDS             complement(3582..4544)
                     /locus_tag="VGB68_18200"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY0051233.1"
                     /translation="MKALRFENNNLKLTEIARPESETEAVVRVLKSGICNTDLEIVRG
                     YAGFQGTIGHEFVGVVEKAFGAPHLIGKRVVGEINAGCGVCASCAAGDSRHCPKRTVL
                     GIVGRDGCHAEFLTLPVRNLLEVPENVSDAEAVFAEPLAAAFGIGEQVEIFPDTEAAV
                     IGDGKLGTLCALGLALKSNKVVLIGKHREKLAIAEKRNVEGVLLADALKMKKRFEVVV
                     EASGSESGFALALDLVKPRGKLILKSTFQGTPRWEAWRVVVDEITIVGSRCGRFAPAL
                     DLLKNKQIDVADLISEEFDLKDGVRAIERAGEKGVMKVLLSITN"
     gene            complement(4836..5312)
                     /locus_tag="VGB68_18205"
     CDS             complement(4836..5312)
                     /locus_tag="VGB68_18205"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0051234.1"
                     /translation="MSADFEIFIRSADNKDCVKVQNLVFGILDEYGLTVETGGTDADI
                     ADIEANYLNRGGAFEVIEDASGNLLGTIGLYPLDAETIELRKMYFDRKLRGRGVGRKM
                     LERMIEKARALGFKRIYLETASVLKAAAALYEKYGFEPTTEGIHSKRCDAAYFLKL"
     gene            complement(5312..>5691)
                     /locus_tag="VGB68_18210"
     CDS             complement(5312..>5691)
                     /locus_tag="VGB68_18210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051235.1"
                     /translation="HIKSYVATLDNYYKESSVHREHVRADRLRAFERYPAINLQIDNI
                     KITPHSTVSATVVFDKTWTFKRPDRTSTGSVQQEMELVKQDGRWLINVEKDSKVYYIN
                     NRDNESNNTNQNADSGNSDADSN"
BASE COUNT         1206 a         1534 c         1441 g         1510 t
ORIGIN      
        1 cctctttcta tctgaattta caatgttggc tcacagaaaa atgctatgca gattcattaa
       61 atcaatccta taacgaacta caaatattag gtaaattatc accagtattc gtactgctca
      121 ctttctggtg gggaattgcc atcattcttt ctctaattgc tttatttact aacaaacaaa
      181 gcgatgctcg acaatcaaaa aagtaaggaa atctgagaag atttccttac ttttacgaaa
      241 tttacttttc gttaaatttc gaccaaatca atctgcgctt tttgcagctt gcccgaatag
      301 tctatgtaga gcgatttcca ctcggagaaa atatcgagaa cctgcgttcc ggcttcgcgg
      361 tgtccgttgc cggtgccttt cgtgccgccg aacggcaggt ggacttcagc gccgatggtc
      421 gccgaattga catagcagat tccggtgtaa agctcctgca tcgcataaaa agactggttg
      481 acgtcctgcg tgtaaatcgc cgacgataag ccgtatttca cgtcgttgac gatatcgatg
      541 gcttcatcga gcgtcgaaaa cggaatcacg ctggtgaccg gtccgaaaat ttcttcctga
      601 ccgacgcgca ttttcggcgc gacgtcggtg aaaacggtcg gctcgataaa atagccgttt
      661 ttgtaatcgc ctttggtcag acgatttccg ccgcacgcga gggtcgcttt atcttccttc
      721 tgaccgattt cgatgtagcc gagaatcttt tccatcgcgg cttgattgat gacgggaccg
      781 acttcggttt tttcgtccag accgttgccg acacgcaggc gtttcgcttt ttcgaccagt
      841 ttttcgcaga actttttata aacttttttg tggacgacca gccgcgacga agccgtgcag
      901 cgctgcccgc tggttccgaa cgcgccccag agcgagcctt cgacggcgtt gtcgatgtcg
      961 gcgtcgtcca tcacgataat cgcgtttttg ccgcccattt ccaatgaaac gattttattg
     1021 tcgcgcgcgc attgctcggc gatgattctg cccgtatcgg tcgagccggt aaacgaaatc
     1081 aggcgaatgt cgcgatgatt aaccagcggc gagccgacgt ctgcgccgtg tccgttgacg
     1141 agattgacga cgcctttcgg aattccggct tcttcgcagg atttaattaa atttaaagcc
     1201 gataaaggaa cgtcttcgcc cgatttgatg acgaccgtgt tgccgcaaat caaagccgga
     1261 atcagcttcc acgacggaat cgccatcgga aagttaaacg gcgtaatcaa gccgcagatg
     1321 ccgaccggct ggcgcacgca catcgcaaat ttattcggca tttccgccgg agtcgtaaaa
     1381 ccgtgcaggc gtctgccttc gcccgccgtg taatacgtgc agtcaatcgc ttcctgcacg
     1441 tcgccgccgg cttcttttaa aaccttgccc atctcgcgcg tcatatcgcg cgtatagcgg
     1501 tctttgtttt tcgccaggat ttcgcccaga cgataaagaa tttcggcgcg gcgcggcgcg
     1561 ggcgttcgac gccatttggt cgccgccgct tttgccgctt ccaccgcccg gttgacgtct
     1621 tcttcatttg aaagcggaaa acgcccgata acgtcgttcg tatcggcggg atttatattt
     1681 tcaaaccatt cgccggacgc ggatttcgtc cactcgccgc cgatatagtt gtaaaaggtt
     1741 ttcgttgttg ttttgcttgc cgtttttttt gattttgctt ctgatgcttt ttctgctttt
     1801 gccataattt agtaaatcgg aaatcggaaa tcgggaattg gtatcttttt tcccgatttc
     1861 cggttcacga ccttattgaa ctgtaaattg aacgattctt tgctgcacgg cgcgcgtaaa
     1921 tttattgtaa gacgcgacgt gcagaacgac ggcgtcgccg gtttttaatt tttcggtgat
     1981 gtcgctgaaa gtcttcaaat cggtgacgct cgtgcgattg atgcgctgaa tcaaatcgcc
     2041 cgcgttgagc gcgtcggcgc cgttggtcgc ctttaaatcg gcgataaagc ttgccgggtc
     2101 gatatttttg ataaccagac cgctttgtcc ttcgagcttg taggtcgtcg ccagctgcgg
     2161 cgtaatttca gccagcgtca gaccaagcgg cgcgagcggc gcggcggtcg gagcgacgcc
     2221 gagttttttt cttgtcgtat cgccttcggc ggtcgggctg tttgacggac gctcgccgag
     2281 tttaataacg gtcgtttggc gttccagctt atcgcccgac tcgcgcagat aagcgatatt
     2341 gatttccttg agcggctcgg tcgccgcgac cttggcaatc aaatccagcg cgctgacgac
     2401 ggcttcgccg ttaaattccg tgataacgtc gttgatttgc aaaccggctt tcgacgccgc
     2461 gccggtgcgg tcgcgaatgt cggtgacgat tgcgcctttc gtttcgccga gattgtaaac
     2521 ctttgcaaat tcgggcttga ccgaatcgag attgacgccc agataaccgc gccggacttt
     2581 tccgttcgcc aggatttgct ggtaaacgta agccgcttct ttagacggca gggcaaagcc
     2641 gatgccgttg taatcgccgg tggtcgaggc aatctgcgaa ttgacgccga tgacctcgcc
     2701 gttcatattg acgagcggtc cgccggaatt gccgcgatta atcgccgcgt ccgtctgaat
     2761 aaatctctga aacgaagtgg tataaggcgt ttctcgccgg gtttgcgaaa tgattccggc
     2821 ggtcacggtt tgcgccaagc cgaacggcga gccgaccgcc agaacccagt cgccgacctg
     2881 cacggcgtcg gaatcgccga acttgacggt cggcaaatcc tttccggctt cgaccttcaa
     2941 aacggctaaa tcggtttcgt cgtccgtgcc gacgactttc gcgatgtatt cctcgccgtt
     3001 ttgcagccgc acggaaatgc gcgaagcatc gtcgacgacg tgaaaattcg tcagaatata
     3061 gcccgtttta tcgacaataa aaccgcttcc gaccgagtag gacgggcggc gcggcagctg
     3121 gcgcttgaaa tattccagaa tgtcgtcggg gttcgtcggc tcggtctgct gctcgccttt
     3181 cagcgtaact tccggaactt ttcccttcgt gtcgatattg acgaccgccg gttcgacgcg
     3241 tctggaaact tcggcaaacg aagccgaaag cgtttcgggc gcgacccgca gattttcggg
     3301 cgtttccgct gtgattaagc gcccgcccgc cagcattccc aaaacaatgc ccgacgccag
     3361 cagcagacac gctgcagcta aaactacaaa ccacacgcgc tttgcgcgcc aaatctgttc
     3421 tttaatttct ccgcaatatc ctgccataag ccgtatttct cctaaatctt ttcggcgagt
     3481 aaaatttgca tttccgagta tagcaaaaga caattacgaa ttacgaatta cgaattacga
     3541 gaggattgat ttcccgattc ccaattcgta attcgtaatt tttaattcgt aattgaaaga
     3601 agcactttca tcacgccttt ttcgcccgcg cgctcgattg cgcggacgcc gtcctttaaa
     3661 tcgaattcct cgcttatcaa atcggcgacg tcaatttgct tgttttttaa taaatccagc
     3721 gccggcgcga aacgtccgca ccgcgagccg acaatcgtga tttcgtcaac cacgacgcgc
     3781 caggcttccc atcgcggcgt tccctgaaac gtggatttta aaattaactt gccgcgcggc
     3841 ttgactaaat caagcgccag cgcaaagccc gactccgagc cgctcgcctc gacgacaacc
     3901 tcaaaacgct ttttcatttt caaagcgtcc gccagcaaaa cgccttcgac attacgcttt
     3961 tcggcgatgg cgagcttttc gcgatgcttg ccgatgagga cgactttatt tgattttaaa
     4021 gccagaccga gcgcgcagag cgtgccgagc ttgccgtcgc cgataaccgc cgcttcggtg
     4081 tcgggaaaaa tttccacctg ctcgccgatg ccgaaagcgg cggcgagcgg ctcggcgaaa
     4141 accgcttccg cgtcggaaac gttttcggga acttccagaa gatttcgcac gggcaaagtc
     4201 agaaattcgg cgtgacagcc gtcgcgcccg acgattccca gaaccgtccg cttcgggcaa
     4261 tgccgcgaat cgcccgcggc gcacgaagcg caaacgccgc agcccgcgtt gatttcgccg
     4321 acgacgcgct tgccgattaa atgcggcgca ccaaaggctt tttcgaccac gccgacaaat
     4381 tcgtgaccga ttgttccctg aaatccggcg tagccgcgca cgatttcgag gtcggtattg
     4441 cagatgcccg atttcaaaac gcggacgacg gcttccgtct cgctttcagg gcgggcaatt
     4501 tcggttaatt tcagattgtt attctcaaat cttaaagctt tcattgttag tcggaacaaa
     4561 aaatcggcgt ttagttgaga tttcgagcct tgcaggcgat ctctgcaaaa gcgcagccgc
     4621 gcattttgac tttgccgaca atcattttaa gctcgcctgc atctctttta attctgtcga
     4681 caatcgtttt aagttcaacg acgccggttt cggttctcct gactcataat tgcatctcgc
     4741 cggcggtcat ctccgtttcg atgaagctcg tttccggatg ttttaactgt tatattttaa
     4801 ctgtttttac ggggcaggcg aatgattttt cccgtttaca atttcagaaa ataagccgcg
     4861 tcgcagcgtt ttgagtgaat gccttcggtc gtcggctcga aaccgtattt ttcgtaaagc
     4921 gcggcggcgg ctttcaaaac gctcgccgtt tccagataga tgcgtttgaa gccgagcgcg
     4981 cgggcttttt caatcattct ttccagcatc tttctgccga cgccgcgccc gcgcaatttc
     5041 cggtcgaaat acattttccg aagctcgatc gtttccgcat cgagcgggta aagcccgatt
     5101 gtgccgagca agtttcccga cgcgtcttcg ataacctcaa atgcgccgcc gcgattcaga
     5161 taatttgctt cgatgtcggc gatgtcggcg tccgtgccgc cggtttcgac cgttaagccg
     5221 tattcgtcca gaatgccgaa aaccagattt tgcactttca cgcaatcctt gttatcggcg
     5281 gagcgaataa agatttcaaa atcggcgctc attaatttga atcggcgtcg gaattgccgc
     5341 tgtcggcgtt ttgattggtg ttgttcgact cgttatcgcg gttgttgatg taataaactt
     5401 tcgagtcttt ttcgacgttt atcagccatc tgccgtcctg tttgacgagt tccatctcct
     5461 gttgaacgga gccggtcgag gttctgtccg gtcttttaaa cgtccaggtt ttatcgaaaa
     5521 cgacggttgc cgaaacggtc gaatgaggcg tgatttttat attatcaatc tggagattaa
     5581 tagccgggta gcgctcgaag gcgcgcaggc ggtcggcgcg gacgtgctct cggtggacgc
     5641 tgctttcctt gtaataattg tccagcgtgg cgacatagga tttgatgtgc g
//
LOCUS       DASWNE010000933         6576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_278738, whole genome shotgun sequence.
ACCESSION   DASWNE010000933 DASWNE010000000
VERSION     DASWNE010000933.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6576
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278738"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VGB68_18215"
     CDS             complement(<1..208)
                     /locus_tag="VGB68_18215"
                     /inference="COORDINATES: protein motif:HMM:NF024759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HEY0051236.1"
                     /translation="MLYEDITKKIFEACFEVSNEPGIGYPESVYEKALLIALRQKSLK
                     AENQIPLQVKFRGEVVGEFFVDILV"
     gene            complement(260..1780)
                     /locus_tag="VGB68_18220"
     CDS             complement(260..1780)
                     /locus_tag="VGB68_18220"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEY0051237.1"
                     /translation="MKRIPLLFLNVIIIATCGLIYELLAGTLASYVLGDSVTQFSLII
                     GIYLFAMGVGSWLSGFLDKNLARRFLEIELAVAIVGGFSAPILFLTFANVSYFNLILY
                     SMVFAIGTLVGLEIPLLMRILKDELDFKKLVSRVLALDYAGALVASLLFPIFLVPKLG
                     LNRTSLLFGMLNAGVGVWGTWLLENLLKKRDVTILRINGFIIILLLVIAFIKADTLTS
                     LAEDALFPDNIIFTKSSSYQRIVVTKGKTGYSLFLNGNLQFNSFDEYRYHEALVHPAF
                     AAFGANPKRILVLGGGDGLAVREILKYPSIESVTLVDLDPEMTKVSKSVPALGELNNH
                     SLEDARVSIINNDAFVWLDNNEIEPFDVAIVDFPDPNNFALGKLYTTRFYNLLKSKLK
                     PESAVVIQTTSPLFARRSYWCIIKTMETAGFSVKPFQTTVPSFGIWGYALAKIEPFET
                     PSKLSGNIELKFLNEQSFASMFVFPADISRIEDVEINRLDNQALVRYYEAEWRRFE"
     gene            complement(1870..2124)
                     /locus_tag="VGB68_18225"
     CDS             complement(1870..2124)
                     /locus_tag="VGB68_18225"
                     /inference="COORDINATES: protein motif:HMM:NF015927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HEY0051238.1"
                     /translation="MLNAIFKFHALAMIVKLDALLEVLVTTLIFVVIGIIFFAITYAI
                     LGRMFPIHKEIEEDQNTALGIVIGSIMIGIAIIIAAAING"
     gene            complement(2129..2278)
                     /locus_tag="VGB68_18230"
     CDS             complement(2129..2278)
                     /locus_tag="VGB68_18230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051239.1"
                     /translation="MLKTLFLVFSGGVLLLYLSSSWFGWEIANSGKNSRFGTPFFYGG
                     FRGGK"
     gene            complement(2401..4344)
                     /locus_tag="VGB68_18235"
     CDS             complement(2401..4344)
                     /locus_tag="VGB68_18235"
                     /inference="COORDINATES: protein motif:HMM:NF025158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HEY0051240.1"
                     /translation="MSVLKANCPSCAAPIEFKAGSTIVLVCPFCRSAVARTDRALEDL
                     GKVAEIVDSQSPLRLGLKGEWRGHRFELTGRAQLRHELGGVWDEWYATFSNGWVGWLA
                     EAQGKFYLTFFQKLPDGAQLPPFERLQLGQALTEIPSKTPLMVTEKGTAASVAADGEI
                     PYQLVPNERSNYADLAGAEHAFGTIDYGMNPPLAFVGEQVSLADIGLANARPAEREAR
                     RVASAAMGCPNCGGPLNLAAPDKSERVTCPNCDSLLEVNQGNLRWLGALKPSPDQHKF
                     DLEIGLKGAFKDGVEMTIIGAVVRSVTVEGIQYFWHEYLLYNPQIGFRWLTHSDNHWN
                     YVEPVNAAEVTSLGNTATYGGKTFKIFQDAPARVEYVKGEFYWRVEVGETVRAIDYIK
                     APLMLSCETTQNEMNWSLGTYMTRAEIEKIFGVQNLAAPWSVAPNQPYTGGFYIKWGF
                     AMLGLLCIVAIFMLPLTGSTATVMSESLTLQPTTGATTPQTVFSQPFQLKGNRNVRIS
                     ASAPVSNSYAELDIDLVNEQNNEVESVSIPIEYYQGVEDGESWTEGGQTNDSTISSLP
                     AGKYMLRLEGTWQNFQQPLPVAVKVEQNVTRGMNFCCAFLLLALVPFFSLIRKWMFES
                     RRWSESMFSGGNTGGDSSGGDDE"
     gene            complement(4341..4733)
                     /gene="speD"
                     /locus_tag="VGB68_18240"
     CDS             complement(4341..4733)
                     /gene="speD"
                     /locus_tag="VGB68_18240"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:TIGR03330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HEY0051241.1"
                     /translation="MLVGTEWIIDAFDCDAEKLRDIAALRAVFSLIVKDLQLKVIDEV
                     WHQFPGERGVTGFNLLSESHLACHTYPENATATFNLYCCRTRPEWNWTKNLTEMLGAE
                     KVSIQKVERGRSPSEIRNPKSKIAGGEA"
     gene            4874..5782
                     /locus_tag="VGB68_18245"
     CDS             4874..5782
                     /locus_tag="VGB68_18245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051242.1"
                     /translation="MIFSACLSISSNDSESKKTGSAADNQKTGDYKEPKIVGTIDSDE
                     ISESSGLVNSPCQPDVLWTHNDSGGSAFIFALDKTGKKLATFKVGGAKNIDWEDLAIR
                     KEQSECFLYIGEIGNNSRSRGEFAVYRVKEPSLSGKKESSKKKPLSTETAEAVKFEYP
                     ELRHDAETLLVHPGSGDIYVLTKRLSGASAVYKLKAGFDLNKTNTLEKIADFAVPAIP
                     NGLLTGGSISPDGKRVVVCDYFAAYEIVLPKQAKNFDEIWKQKPQKIELGQRAQGEAI
                     AYAADGKAIYATSEEKGSPLIEVKRK"
     gene            complement(5784..6533)
                     /locus_tag="VGB68_18250"
     CDS             complement(5784..6533)
                     /locus_tag="VGB68_18250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0051243.1"
                     /translation="MKLLFCPLILIFAFSGSVFAQTGKGIELYNQGDYESAIKILEKA
                     IDENRNDVTAFYYLGLNQEKLYQPKKAAEYYARAVDGWIKLAEATIEAELGKASLEFP
                     RESMAFYVRNKLPGELQTAVESARKFEQLNSKKASAKDWKIKILTLKFFSEPSESQKK
                     TADKKLNGKSIADSTRAANEKPKVGIIRFWILFLSNGEVGMVVPIKRLSETLTEQAFQ
                     MARKIKFEPKIENGKAVSTAKIVEYTVETTN"
BASE COUNT         1696 a         1699 c         1606 g         1575 t
ORIGIN      
        1 caactaaaat atcaacaaaa aactcgccga caacttcgcc tcggaattta acctgtaaag
       61 gaatttgatt ttcagccttt aaactttttt gccgcagagc aataagcaaa gccttttcat
      121 aaacagattc cggatagccg attcccggtt cattactaac ttcaaaacac gcctcgaaaa
      181 tcttctttgt tatgtcttca taaagcattt tacctatctc caaaaatccc tgtttatcct
      241 gttatccctg taaattctct tattcaaatc tgcgccattc ggcttcgtaa tatctgacca
      301 gcgcctgatt gtcgaggcgg tttatctcga cgtcttcgat tctggaaatg tctgccggaa
      361 agacgaacat tgaggcgaaa gattgttcgt ttagaaattt cagctcgatg tttccggaca
      421 atttcgaagg agtttcaaac ggctcgattt tcgcgagcgc gtagccccag atgccgaagc
      481 tcggcacggt cgtctggaaa ggtttcacgg aaaatccggc ggtttccatc gttttgataa
      541 tgcaccagta ggaccggcgc gcgaaaagcg gcgaggtcgt ttgaatgacg accgccgatt
      601 cgggcttcag tttcgatttc agcagattat aaaaccttgt cgtataaagc tttccgagcg
      661 cgaaattgtt cgggtcggga aaatcgacga ttgccacgtc gaaaggctcg atttcgttgt
      721 tgtcgagcca gacgaaagcg tcgttattga taatcgagac gcgcgcgtct tcgagcgaat
      781 ggttatttaa ttcgcccaga gccggaaccg atttcgaaac tttcgtcatt tccgggtcga
      841 ggtcgacgag cgtcacggat tcgattgacg gatatttcag aatttcgcgg acggctaaac
      901 cgtcgccgcc gccgagaacc agaattcttt tcggattcgc gccgaaagcg gcgaacgccg
      961 gatgaacgag cgcttcgtga tagcgatatt cgtcaaaaga attaaattgc agattgccgt
     1021 ttagaaaaag cgagtagccg gttttgcctt tggtgacgac aatccgctgg taagacgagc
     1081 ttttggtgaa aatgatgttg tcgggaaaaa gcgcgtcctc ggctagagaa gtgagcgtat
     1141 cggctttgat aaacgcgatg acgagcagca gaatgatgat gaagccgttg attcgcagaa
     1201 tcgtcacgtc gcgctttttg agcagatttt ccagaagcca cgtgccccag actccgacac
     1261 cggcgttgag catcccgaaa agcagagagg tgcggttaag cccgagcttc gggacgagaa
     1321 atatcgggaa caaaagcgag gcgaccagcg cgccggcgta atcgagcgcc agcacgcgcg
     1381 agacgagctt tttaaaatcc agctcgtcct ttaaaatccg catcaaaagc ggaatttcca
     1441 gcccgacgag cgttccgatg gcgaaaacca tcgaatagag aattaaatta aaataggaaa
     1501 cgttggcgaa agtcagaaaa agaatcggcg cgctgaatcc gccgacaatg gcgaccgcca
     1561 gctcgatttc cagaaagcgg cgggctaaat ttttatcgag aaagcccgac agccacgagc
     1621 cgacgcccat cgcgaaaaga taaatgccga ttatcagcga aaactgcgtg accgaatcgc
     1681 cgagcacgta actggcgagc gttccggcaa gcaattcata aatcaagccg caggtggcga
     1741 tgatgatgac gtttagaaat aaaagaggaa ttcttttcat ttttgtaacg aaaaatgcaa
     1801 aacggaaaac gggaaacgaa ataaacagca atcattttcc attttccact ttccgttttc
     1861 cgacaaaatt tacccgttta tcgcggcggc gatgatgatg gcgatgccaa tcattatcga
     1921 accgatgacg atgccgagcg ccgtattctg gtcttcctcg atttccttgt gaatcggaaa
     1981 cattctgccc aaaatggcgt aggtgatggc aaagaaaatt atgcctatga cgacgaaaat
     2041 caaggtcgtc accaaaactt ccaacaacgc atccagttta acaatcatcg ccaaagcgtg
     2101 aaatttaaaa attgcattca acatttaatt attttcctcc acgaaacccg ccgtaaaaaa
     2161 aaggcgttcc aaatcttgaa ttttttcccg aattggcaat ctcccagcca aaccaggatg
     2221 acgaaagata aagcagaagc actccgccgc tgaaaaccag aaaaagtgtt tttaacataa
     2281 attcgtcctt tgtcctgctg cgtgctttgc tctttgtctt ttacaggtcg aaataaatta
     2341 acgggtgtca ttttccgtaa acctgcgaat gacgaaggac aaagcgcgcg gaactaataa
     2401 ttattcatca tcgccgccgc ttgaatcgcc gcccgtattt ccgccgctaa acatactttc
     2461 gctccagcgc cgcgattcaa acatccattt gcgaatcagc gaaaagaacg gcacgagcgc
     2521 cagcagcaga aaagcgcagc agaaattcat cccgcgcgtg acgttctgct cgaccttgac
     2581 ggcgacgggc agaggctgct gaaaattctg ccaggtgcct tcgagccgca gcatatattt
     2641 tcccgccgga agcgacgaga tggtcgaatc attcgtctgc ccgccttcgg tccaggattc
     2701 gccgtcttcc acgccctgat aatattcgat cggaatactg accgattcga cctcgttgtt
     2761 ctgctcgttg accaaatcaa tatctaattc cgcataggaa ttgctgaccg gcgcggaagc
     2821 ggaaattctg acattgcgat ttcccttcag ctgaaaaggc tggctgaaaa ccgtctgcgg
     2881 cgtggtcgcg ccggtcgtcg gctggagcgt caggctttcc gacattacgg tcgccgtcga
     2941 gccggtcaga ggcagcataa aaatggcgac gatgcacaga agtccgagca tggcaaaacc
     3001 ccatttaata tagaaaccgc ccgtgtaagg ctggttcggc gcaaccgacc agggcgcggc
     3061 gagattctgg acgccgaaga ttttctcgat ttccgcgcgc gtcatatacg ttccgagcga
     3121 ccagttcatc tcgttctgcg tcgtctcgca cgagagcatc agcggagctt ttatgtaatc
     3181 aatcgcccgg acggtttcgc cgacctcgac gcgccagtaa aattcgcctt tgacgtattc
     3241 gacgcgcgcc ggggcatcct gaaaaatctt aaaggttttg ccgccgtaag tcgccgtatt
     3301 gccgaggctc gtgacttccg ccgcgttgac cggctcgacg taattccagt gattgtcgga
     3361 atgcgtcagc cagcgaaaac cgatttgcgg attgtagagc aggtattcgt gccagaaata
     3421 ttggatgcct tcgaccgtca cgctgcgcac gaccgcgccg ataatcgtca tctcgacgcc
     3481 atctttaaac gcgcctttca gcccgatttc caaatcgaat ttatgctggt cgggcgaagg
     3541 cttcagcgcg cccagccagc gcagatttcc ctgatttact tcgagcagcg aatcgcagtt
     3601 cggacacgtc acgcgctcgg atttgtccgg cgcagccaga tttaaaggtc cgccgcaatt
     3661 cggacagccc atcgccgccg aagccacgcg ccgcgcttcg cgttcggcag gtcgcgcgtt
     3721 cgccagaccg atgtccgcca gcgaaacctg ctcgccgacg aacgcgagcg gcggattcat
     3781 accgtaatca atcgtcccga aagcgtgttc agcgcccgcc aaatccgcgt aattgctgcg
     3841 ctcgttcggc acgagctgat aaggaatttc gccgtccgca gcaaccgaag ccgccgtccc
     3901 tttttccgtc accatcagcg gcgtcttact cggaatttcc gtcagggctt gccccagttg
     3961 caaacgttca aacggcggaa gctgcgcgcc gtccggcaat ttctggaaaa aagtcagata
     4021 aaatttccct tgcgcttcgg cgagccagcc gacccagccg tttgagaaag tcgcgtacca
     4081 ttcgtcccag acgccgccga gctcgtgccg gagctgcgcg cgtcccgtca gttcaaaacg
     4141 atgcccgcgc cattcgcctt ttaagccgag ccgcagaggc gactgcgaat cgacgatttc
     4201 ggcgactttt cccaaatcct ccagcgcgcg gtcggtgcgc gcgaccgccg aacggcaaaa
     4261 cgggcaaacc agcacaatcg tcgagccggc tttaaattcg atcggcgcgg cacacgaagg
     4321 acaattcgct ttcagaacgc tcaagcttca cctccagcga ttttggattt cggatttcgg
     4381 atttcggatg gcgaacgccc gcgctcgact ttttgaatcg acaccttttc ggcgcccagc
     4441 atttcggtta aatttttcgt ccagttccat tcgggacgcg tccggcagca atataaatta
     4501 aacgtcgccg ttgcgttttc gggataagtg tgacaggcga gatgcgattc gctcaaaaga
     4561 ttaaagcccg tcacgccgcg ctcgccggga aattgatgcc agacctcgtc gatgactttt
     4621 aattgtaaat ctttgacgat tagcgaaaaa acggcgcgca aggctgcaat atcgcgcagt
     4681 ttttcggcgt cgcaatcgaa ggcatcaatt atccattcag ttccaacgag cattaattga
     4741 ttttacgaaa aaagatttat aaagtttact gaaccttgca aaacgaatcg gcgttataat
     4801 cgtttgacga agtatgttga aaagaaatat acccaaaatc ttcgcttatg tcatttgctt
     4861 cggcgcaatt tgcgtgattt tttccgcttg tctttcaatt tcttcaaacg attcggaatc
     4921 aaagaaaacg ggaagcgccg ccgacaatca gaaaaccggc gattataaag agccgaaaat
     4981 cgtcggcaca atcgattccg acgaaatttc cgaaagcagc ggcttggtaa attcgccttg
     5041 ccagcccgac gttttatgga cgcacaacga ttccggcggc agcgccttta tcttcgcgct
     5101 cgataaaacc ggaaaaaaac tcgccacttt taaagtcggc ggcgcgaaaa atatcgattg
     5161 ggaagatttg gcgattcgca aagagcaatc ggaatgtttt ttatacatcg gcgaaatcgg
     5221 caataactcc cgctcgcgcg gcgaattcgc cgtttacagg gtcaaagaac ccagcctttc
     5281 cggcaaaaaa gagtcaagca aaaaaaaacc gctttcgacc gaaaccgccg aagctgtaaa
     5341 attcgagtat cccgagttgc ggcacgacgc cgaaactttg ctggtgcatc cgggaagcgg
     5401 cgacatttac gttttgacaa aacgcttgag cggcgcgtca gccgtttata aactcaaagc
     5461 cggatttgat ttgaacaaaa cgaatacgct ggaaaaaatc gcggatttcg ccgttccggc
     5521 tattccgaac gggcttctga ccggcggctc gatttcgccc gacggcaagc gcgtcgtcgt
     5581 ctgcgattat tttgcggctt acgaaatcgt cttgccgaaa caggcgaaaa attttgacga
     5641 aatctggaaa cagaagccgc agaaaatcga gctcggacag cgcgcgcagg gcgaagcgat
     5701 tgcttacgcg gcggacggca aggcgattta tgcgacgagc gaggaaaaag gttcgccgct
     5761 gatcgaggtt aaaagaaaat aaatcaattc gtcgtttcaa ctgtgtattc cacgattttt
     5821 gccgtcgaaa cggctttccc gttctctatt ttcggctcaa actttatctt tcgcgccatc
     5881 tgaaaggctt gttcggttaa agtttcggaa agacgcttta tcggaacgac catcccgacc
     5941 tcgccgttcg agagaaataa aatccaaaac cgaataatcc cgactttcgg cttttcgttc
     6001 gccgcgcggg ttgaatcggc aatggattta ccgttcagtt ttttatcggc agtctttttt
     6061 tgggattcgg acggctcgga aaagaatttc aaggtcagaa tttttatttt ccaatctttc
     6121 gcgctcgcct ttttggaatt taactgttcg aatttccggg cgctttcgac ggctgtctga
     6181 agctcgcccg gcaatttgtt gcgaacataa aaagccatgc tttctctggg aaattcgagc
     6241 gaagcttttc cgagttccgc ctcgattgtc gcttcggcga gctttatcca gccgtcgacg
     6301 gcgcgggcgt agtattctgc ggcttttttc ggttgataaa gtttttcctg gtttaagccg
     6361 agataataaa aagcggtcac gtcattccgg ttttcgtcga ttgccttttc cagaatttta
     6421 atcgccgatt catagtcgcc ttgattataa agctcgatgc ctttgccggt ctgggcaaaa
     6481 accgagcccg aaaaagcgaa tattaaaatc agcggacaaa ataacaattt cataaatata
     6541 gcctttcaat gctttcaaaa taattttaag caattt
//
LOCUS       DASWNE010000934         1503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_452621, whole genome shotgun sequence.
ACCESSION   DASWNE010000934 DASWNE010000000
VERSION     DASWNE010000934.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452621"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1503)
                     /locus_tag="VGB68_18255"
     CDS             complement(<1..>1503)
                     /locus_tag="VGB68_18255"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HEY0051244.1"
                     /translation="ASGVSAAYAQPEKNPSQNPADKPRNVKKEPVNKVYRDWVSKDVA
                     YIITKDEKKAFDKLTTDEERENFIENFWRRRDPNPDTEENEYREEFYERIAYANEHFT
                     SGIPGWKTDRGRVYITFGKPDSVESHPSGGAYDRPSYEGGGSTTTYPFEIWFYRHLDS
                     VGEGIEIEFVDPTGTGEYRIARSPYEKDAMAMVPGAGLTTAEQLGLSDKSQRNGAGGQ
                     NFMREQDNPFRRMEIIANLQRPPQVKFSDLQANLESPVIDNNPLDFDLRVDFFRQSDE
                     RVVTAFTIQTDNKELVFEQIGGLETARMNIFGRITAVAGKRSGIFEDSVTTNASPAEL
                     AEARDRKSIYQKAIALTPGTYKVDVVVRDVSSGSRGIRTLGFTVPKYEAEKLSASSLI
                     LASKLYSTTEKDIGSMFVVGNAKVIPNLTGVYKRGQEVGIYMQIYNAGIDQTTLRPSV
                     DVDYILLKDGKEVFRQKEDWQGLSDSGQRLTIARLLPTTMLPLGEYEIKIS"
BASE COUNT          326 a          395 c          380 g          402 t
ORIGIN      
        1 gaaattttga tttcgtattc gccgagcggc agcatcgtcg tcggaagcag gcgggcaatc
       61 gtcaggcgct gtcccgaatc gctcaagccc tgccaatctt ctttctgccg gaaaacttcc
      121 ttgccgtctt tcaacagaat ataatcgacg tcaaccgatg gacgcagagt cgtctggtcg
      181 attccggcgt tgtaaatctg catatagatg ccgacttcct gacctctctt gtaaacgccg
      241 gtcagattcg gaataacctt tgcgtttccg acgacaaaca tcgagccgat gtctttttcg
      301 gtcgtcgaat aaagcttgga agccagaatc aggctcgaag ccgagagttt ttccgcttcg
      361 tatttcggaa cggtaaaacc gagcgtgcgg attcctctgc tgcccgaaga aacatcgcgg
      421 acgacgacgt cgaccttgta tgttccgggc gttaaggcga tggctttttg ataaatggat
      481 tttcggtctc tggcttcggc aagctcggca ggcgaagcgt tggtcgtgac cgaatcttca
      541 aaaatgcccg aacgctttcc ggcaactgcc gtgatgcgcc cgaaaatatt cattctcgcc
      601 gtttccagcc cgccgatttg ttcaaagaca agctctttat tatcggtctg aatcgtaaag
      661 gcggtaacta cgcgctcatc cgattgacgg aagaaatcta cgcgcaaatc aaaatccagc
      721 ggattattat caatcaccgg agattccaga ttagcctgca aatcgctgaa ttttacctgc
      781 ggcggacgct gcaggttggc aataatttcc atccgtcgaa acgggttgtc ctgttcgcgc
      841 ataaaattct gcccgcccgc cccgtttcgc tgcgatttat cgctcaagcc taattgttcg
      901 gctgtagtca aaccagcgcc gggaaccatc gccatagcat ctttttcata cggcgagcgg
      961 gcgatgcgat attcgccggt tccggtcggg tcgacaaatt cgatttcgat tccttcgccg
     1021 acgctgtcga gatgccggta gaaccaaatt tcgaacgggt aagtcgtcgt cgaaccgccg
     1081 ccttcgtagc tcggacggtc gtaagcgccg ccggacggat gcgattcgac ggaatccggc
     1141 ttgccgaatg tgatatagac gcgaccgcgg tcggttttcc agccgggaat gcctgaagta
     1201 aagtgttcgt ttgcataagc gatgcgttcg taaaactctt cacgatattc gttttcttcg
     1261 gtatcgggat tcgggtcgcg gcgacgccag aagttttcga taaaattttc gcgctcttcg
     1321 tcagtggtta acttatcaaa cgctttcttt tcgtctttgg taataatgta ggcgacatcc
     1381 ttgctgaccc aatcgcggta aactttatta accggttctt tttttacatt tctgggttta
     1441 tctgccggat tctggctcgg atttttttcg ggctgggcat acgccgccga aactcccgaa
     1501 gcc
//
LOCUS       DASWNE010000935         3944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_452647, whole genome shotgun sequence.
ACCESSION   DASWNE010000935 DASWNE010000000
VERSION     DASWNE010000935.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452647"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VGB68_18260"
     CDS             complement(<1..474)
                     /locus_tag="VGB68_18260"
                     /inference="COORDINATES: protein motif:HMM:NF020768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0031221 - arabinan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinofuranosidase catalytic domain-containing
                     protein"
                     /protein_id="HEY0051245.1"
                     /translation="MILIKNFPALLLLSIFSLSCFPAAARAEFSMPAAINRRPKTYVL
                     DQISARPLAAYSTRRLRRTYVGAAIRVRRLSDGAEIDLVAGFSKAMADAFGSNLQIIK
                     IYDQSGNGRDLAIVGTPPKLSYLARPMIDFYECNDSTEFRSAATINLKEPTQFFAI"
     gene            complement(878..1717)
                     /locus_tag="VGB68_18265"
     CDS             complement(878..1717)
                     /locus_tag="VGB68_18265"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HEY0051246.1"
                     /translation="MKVESSKTNQAAERKVSSQNKNVGQTNVLLNKNDLTNSAPPPSK
                     SFAQILEETRNQNGEDEGFPINRKSDRARESADSDEAKNEKETSRDVLTRDAVEERER
                     KESDERQSDDGESNSGFGAFVFQADNKIYNSNSIPAARSILHVADLERIVSAIRAQNL
                     KNEQAIIISLKHSVLEGLQIKLTIEENGKLKAEFLAASEQIKNQLDARKDELTSIIRE
                     RGINLSEMSVRQGSDFSQNSETDENSLAEKNISSPEAAELSPDKISEEPDDEPNTHIS
                     YRV"
     gene            complement(1745..3094)
                     /locus_tag="VGB68_18270"
     CDS             complement(1745..3094)
                     /locus_tag="VGB68_18270"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0051247.1"
                     /translation="MSSPGKPFNFSENLTSKFRALLFLSLFGALSVIIHAQDDLRNTV
                     YGGGSKPSKTKKTASTTPKTAPMPKKRKPRLPAKTNLLPVVFTTQEARAEIWLNNRNI
                     GVTDGAAQLRRSLAPGLYQVMVKKGGQILNPVQSISVTPEQTDFLLVREAPKQNRQTT
                     PVVKTENPKPKSEQEIALEISQKVRETLENYADPTKTHSVTTADWELVLQAAQLGQLQ
                     NYTAVQIEAQRWFASGQIELARGNYPNAFKAFNQAVEYMPNSALPFYGLGITYLANKQ
                     HVDALKAFQKALQADSKNAMAHRGFADALRLTGKEKEAINSYKYAIQFGYRTPETRYY
                     LATMLSKTGKHPDAIKELDEVAKEAPNADVYIALGTAYQALKRDVSALEAYQKATELD
                     PNSAIAFDNLGDVYYKQREYVKAKDAFEKAVALDPEGKSLNLKQTQNKIREATSKIK"
     gene            complement(3188..>3944)
                     /locus_tag="VGB68_18275"
     CDS             complement(3188..>3944)
                     /locus_tag="VGB68_18275"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEY0051248.1"
                     /translation="TQPSPASQPVEPNPPAETNIETARPENVVDVAGGEITLGGGETG
                     RPLKRILVEDFAIAETEVTNEQYAEFVKATNHRAPSGWKNNEFPEGAADFPVSGVSFA
                     DAEAFCKWLAAKINLPVRLPSEAEWEYAARGRENNKYPWGNEWDAKGAASSETGGKVS
                     AVKSFPLNRSPFGAFDMAGNIWEWTQDKVSETDVTDEQVRAALKSGKILRVVKGGSAT
                     EKAAQISARSRFEIPEQTKVATVGFRYVVGRKR"
BASE COUNT          765 a         1028 c         1022 g         1129 t
ORIGIN      
        1 gatggcaaaa aactgcgtcg gctcctttaa attaatggtc gcggcggaac gaaactcggt
       61 cgagtcgttg cactcataaa aatcaatcat cgggcgggcg agatagctca gcttcggcgg
      121 cgttccgaca atggctaaat cgcgtccgtt gccgctctgg tcatagattt taatgatttg
      181 cagattgctg ccgaaagcgt ccgccatcgc tttcgaaaac ccggcgacga ggtcgatctc
      241 ggctccgtcg gacaaacgtc tgacgcgaat cgccgcgccg acatacgtcc gtcgcaggcg
      301 gcgcgtcgaa tacgcggcga gcgggcgagc cgaaatctgg tcgagcacat aagttttcgg
      361 gcgtcgattt atcgccgccg gcattgaaaa ctcagcccgc gccgccgccg gaaaacacga
      421 aagcgagaaa atcgacaaaa gcagaagtgc cggaaaattt tttatcagaa tcataatgct
      481 ccaatttact cgataaaagt cggttgctcg ttgaaagacg cgttctgcgg caatgccgcg
      541 ctagagagcg gcggcgacgc gcgaatattt cgatttatcg aagatagaac ccgcgcgcga
      601 atcgcggaat tattgcttca ggagtgttta ttcaatttca gttttcgttt gaatttattt
      661 ttgtgaacaa atagatgcgg ctttaatctg ccgcctgctg cgcggacgtc ttgataaata
      721 acatagcaga tgtttttgat gaaaatcaac aagttttaac agaatgggat ttttttttcg
      781 cgaggctcac ctatagccgg cggcgcgcgt tgatttaaat caacgcgcgc cggatgataa
      841 gctcattcgt tttcgtattt aagtcgattt ggcgcgatta gacgcggtag gaaatatgag
      901 tgttcggttc gtcgtccggc tcttcggaaa ttttgtcggg cgaaagctcc gcggcttccg
      961 gcgacgaaat atttttttcg gcgagcgaat tttcgtcggt ttcgctgttt tgtgaaaaat
     1021 ccgagccttg ccggacgctc atttcggaaa gatttatgcc gcgctcgcgg ataatgctcg
     1081 tcaattcgtc tttgcgcgcg tcgagctgat tttttatctg ctcgctggcg gctaaaaatt
     1141 ctgcttttaa tttgccgttt tcttcgattg tcaattttat ctgcaagcct tccagaaccg
     1201 agtgtttgag cgaaattatg attgcctgct cgtttttcag attttgcgcg cggattgcgg
     1261 aaacgattct ttcgaggtcg gcgacgtgca gaatcgagcg cgccgccgga atcgaattcg
     1321 agttgtagat tttattatcg gcttgaaaaa caaatgcgcc gaagccggaa ttggactcgc
     1381 cgtcgtcgga ctgccgttcg tccgattctt ttctctctct ttcttcgacc gcgtcgcgcg
     1441 tcagcacgtc gcggctcgtt tccttttcgt ttttggcttc gtcggagtcg gcgctttctc
     1501 tggcgcggtc ggattttcga ttaatcggaa agccttcgtc ctcgccgttc tgatttcgcg
     1561 tttcttccag aatctgcgcg aagcttttcg agggcggcgg cgcggaattc gtcaaatcgt
     1621 ttttgtttaa taaaacattt gtttgaccga cgtttttatt ctgggaagaa acttttctct
     1681 ccgccgcctg attcgttttt gatgattcga ctttcataaa aattctccgc cgaaaatttt
     1741 caaattattt tatttttgaa gttgcctcgc gaattttatt ctgcgtttgc ttcaagttca
     1801 gactttttcc ttcggggtcg agcgcgacgg ctttttcaaa cgcgtctttc gccttcacat
     1861 actcgcgctg cttgtaataa acgtcgccga gattgtcgaa agcgattgcc gaattcgggt
     1921 cgagctcggt cgctttctga taagcttcaa gcgcgctgac atcgcgcttg agcgcctggt
     1981 aagcggtgcc cagagcgatg taaacatcgg cgttcggcgc ttctttcgcc acttcgtcaa
     2041 gctcttttat cgcgtccgga tgctttccgg ttttcgacag catcgtcgcc agataataac
     2101 gcgtctccgg cgttcgatag ccgaattgaa tcgcgtattt ataagagttt atcgcttcct
     2161 tttcctttcc ggtcagccgc agcgcatcgg caaaaccgcg atgcgccatc gcgtttttcg
     2221 aatccgcctg cagcgctttt tgaaacgctt tcagcgcgtc gacgtgctgt ttattggcga
     2281 gataagtaat gccgagaccg taaaaaggca gcgccgaatt gggcatatat tcgacggctt
     2341 gattaaaagc cttgaaagcg ttcgggtaat tgcctctggc aagctcgatt tgaccggaag
     2401 cgaaccagcg ctgcgcttcg atttgcaccg ccgtgtaatt ttgcagctgc ccgagctgcg
     2461 ccgcttgcag aaccagttcc cagtcagccg tcgtgacgct gtgggttttg gtcgggtcag
     2521 cgtagttttc cagcgtttcc cgcaccttct ggcttatttc cagagcgatt tcctgctcgc
     2581 ttttcggttt cggattttcc gtttttacca ccggagtcgt ctgcctgttc tgtttcggag
     2641 cttcgcggac taatagaaaa tcggtctgtt cgggcgtcac gctgatcgat tggacgggat
     2701 tcagaatttg cccgcctttt ttgaccatta cctgataaag cccgggcgcg agacttctcc
     2761 tgagctgcgc cgcgccgtcc gttacgccga tgttccggtt gttcagccag atttccgctc
     2821 gtgcttcctg cgtcgtaaag acgaccggca gcaaatttgt tttcgccggc aggcgaggct
     2881 tgcgcttttt cggcatcgga gccgtctttg gcgtggtcga agccgttttt ttagttttcg
     2941 acggcttgct tcctccgccg tagacggtgt tgcgcaaatc gtcctgcgcg tggatgatta
     3001 ccgacagcgc gccgaataac gagagaaata gaagggcgcg aaatttcgac gttagatttt
     3061 cgctaaagtt aaaaggtttt ccggggctgc tcatcttttt acctctttta attacgattg
     3121 ttcgatgttc gtcttattat aatttatcaa gttaaaattt tcccgatttt gttagtaaaa
     3181 ccgcgtctca tcgttttcgt ccgacgacgt aacgaaatcc gaccgtcgcc acttttgtct
     3241 gttcgggtat ctcaaatctc gaacgggcgg aaatctgcgc cgctttttcg gttgccgagc
     3301 cgcctttgac gacgcgcaga atctttccgc ttttcagcgc cgcgcgcacc tgctcgtcgg
     3361 tcacgtccgt ttccgaaact ttgtcctgcg tccattccca gatgtttccc gccatatcga
     3421 aagcgccgaa cggcgagcga ttcagaggaa agcttttgac cgccgaaact tttccgcccg
     3481 tttcgctgga agcggcgcct ttcgcgtccc attcgtttcc ccacggatat ttattatttt
     3541 cgcgcccgcg cgccgcgtat tcccactcgg cttcggacgg caggcgaacc ggcagattga
     3601 ttttcgccgc cagccatttg cagaaagctt cggcgtcggc aaatgaaacg ccgctcaccg
     3661 gaaaatccgc cgcgccttcg ggaaactcgt tatttttcca gccggaaggc gcgcgatgat
     3721 tggtcgcttt gacgaattcc gcgtattgtt cgttggtgac ttccgtctcg gcgatggcaa
     3781 aatcttcgac cagaattcgt ttcagcggtc ttccggtttc gccgccgccg agcgtgattt
     3841 cgccgcccgc gacatcgacg acgttttccg gtctggctgt ttcaatattt gtttcggctg
     3901 gcggattcgg ctcgaccggc tggctcgccg gcgacggctg cgtt
//
LOCUS       DASWNE010000936         3633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_626632, whole genome shotgun sequence.
ACCESSION   DASWNE010000936 DASWNE010000000
VERSION     DASWNE010000936.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626632"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VGB68_18280"
     CDS             <1..244
                     /locus_tag="VGB68_18280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051249.1"
                     /translation="PGGETNLTTQIEVFDTNGRAVISSPVRPLVLKGNNDLARIPLTG
                     AIRQDLIAPGTYLLRVAVNDAIAKTKAVQQTIFTIE"
     gene            292..876
                     /locus_tag="VGB68_18285"
     CDS             292..876
                     /locus_tag="VGB68_18285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEY0051250.1"
                     /translation="MDENDKLFLPVLLGTNRQGRQSEQVARWVFGKMQEREEIETEFF
                     DARDFHLPVDDYGTEIARNFPEWRDAIIKADGLVIVTPEYNHGYPGILKAVLDLLLKE
                     YIHKAVAFVGVSAQVWGGVRVIEACVPMVRELGLAVTFTDLNFPRAHTQFDKDGKLLD
                     EAYNKRVAGFLDELVWMSRALRWGRRNLPSKYHQ"
     gene            1066..1479
                     /locus_tag="VGB68_18290"
     CDS             1066..1479
                     /locus_tag="VGB68_18290"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY0051251.1"
                     /translation="MAEDLAKKFVEALGKLEAERDLETISSLFGDDSEIGNVVTADNK
                     NGIGGARRFWQHYRDNFDRIKSTFRNQIVTGNTAALEWQSEGTSSDGNEFSYEGVSIL
                     EFDGDKISRFHAYFDPNKLGRQIVDEKSREAGNGG"
     gene            complement(1593..2468)
                     /locus_tag="VGB68_18295"
     CDS             complement(1593..2468)
                     /locus_tag="VGB68_18295"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0051252.1"
                     /translation="MTNSFQLSASEAEGDADFNQGSIFFIGTATVILRYAGFTILTDP
                     NFLHAGDHVHLGYGLTSERLTNPAMDIEDLPPLDLCVLSHYHGDHFDRIAEEKLNKDL
                     PIITTNHAREELEAKNFRKAIGLDTWEAVDFTKGDARLRISSMPGQHGPGVVDFALPP
                     VMGSVLDFETLDAERVLRLYISGDTLVMDELKEIPQRFPEIDLGLFHLGGTRIMGVML
                     TMDGEQGVEAIRIIRPEKAIPIHYNDYDVFKSPLEDFKRAAEEAGLSEKIIYLSHGET
                     YNFEVPASRRRQVSA"
     gene            2795..3136
                     /locus_tag="VGB68_18300"
     CDS             2795..3136
                     /locus_tag="VGB68_18300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051253.1"
                     /translation="MPTINRTLRVRTDADGRYETTQTFNPPGFWDYTIRAYAKLIAPA
                     DTTVNARIEISAANHTTDNPPKDFRLSTGETEDLGSWQLDGGDNTVRVTGQTSPARAN
                     EMLEIQVTAEI"
BASE COUNT          980 a          896 c          929 g          828 t
ORIGIN      
        1 cccgggcggc gaaacgaatt tgaccacgca gatagaggtt ttcgatacga acgggcgcgc
       61 cgtcatcagc agtcccgtgc gaccgctggt tttgaaagga aataatgatt tggcgcgaat
      121 tcccctgacg ggcgcgattc ggcaggattt gattgcgccg ggaacgtatc tactgcgcgt
      181 cgcggtcaac gacgcgattg ctaaaacgaa agccgtccag cagaccattt ttactatcga
      241 ataaaaaatt aaattcaaaa taaaaggcga agcttaagaa atataaaaga gatggacgaa
      301 aacgacaaac tttttctgcc cgttttgttg gggacaaacc gccagggacg ccagagcgag
      361 caggtcgcgc gctgggtttt cggaaaaatg caggagcgcg aggaaatcga aaccgagttt
      421 ttcgacgccc gcgattttca tctgcccgtc gacgattacg ggacggaaat cgcccgaaac
      481 tttcccgaat ggcgcgacgc gattatcaaa gccgacgggt tggttatcgt gacgccggaa
      541 tacaatcacg gttatccggg aattttgaaa gcggtgctgg atttgcttct gaaggaatat
      601 attcacaaag cggtcgcttt cgtcggcgta tcggcgcagg tctggggcgg cgttcgcgtc
      661 atcgaagcct gcgtgccgat ggttcgcgag ctgggtttag ccgtcacgtt taccgattta
      721 aactttccgc gggcgcacac gcagttcgat aaagacggca aattactgga tgaagcctac
      781 aacaaacgcg tcgcgggttt tctggacgaa ctcgtctgga tgagccgcgc cctgcgctgg
      841 ggacgccgga atctgccttc caaatatcat cagtaaataa gccgaaaaat cttgactaag
      901 cgcggtgcat agccgatgct attgttatag aaattgttct tcttagtaaa cgcttccccg
      961 agcggtcggc ttgttttcgt atccggagaa aacgggcttg ccgctcgacg tgtatttatt
     1021 tgagcggaca atctctaaaa tttacaaaat aaagggaagg ttaaaatggc agaagattta
     1081 gcgaaaaaat ttgtagaagc gctcggcaaa ctcgaagccg agcgcgattt ggaaaccatc
     1141 agctcattat tcggcgacga ttcggaaatc ggcaacgtcg tcaccgccga caacaaaaac
     1201 ggaatcggcg gcgcgcgccg attctggcag cactatcgag acaatttcga ccgaatcaaa
     1261 tcgacgtttc gcaatcaaat cgttaccggc aacaccgccg cgctcgaatg gcaatccgag
     1321 ggcacaagct cggacggcaa tgaattttct tacgaaggcg tcagcattct ggaattcgac
     1381 ggcgataaaa tctcgcgctt tcacgcctac ttcgacccga acaaattagg tcggcagatt
     1441 gtcgacgaaa aaagcaggga agcgggaaac ggcggctgat aaattgaaaa agcgataagg
     1501 tcaaaagaaa ttgatttgta ttgaacgaga aaaataaaga gaagccttcg gcaaacggct
     1561 tctctttatt tatgtttgtg tcaacggaaa tgttaagccg aaacctgccg gcggcggctc
     1621 gccggaactt caaaattata cgtttcgccg tgactgagat agatgatttt ttcgctcaaa
     1681 ccggcttctt ccgccgcgcg cttgaaatct tcgagcggcg atttaaaaac gtcgtaatcg
     1741 ttgtaatgaa tcggaatggc tttttcggga cgaataatcc tgatggcttc cacgccctgt
     1801 tcgccgtcca tcgtcagcat cacgcccata atccgcgtgc cgccgagatg aaacaaaccc
     1861 aaatcgattt cgggaaatct ctgcggaatt tcctttaatt cgtccatcac gagcgtgtcg
     1921 cccgaaatat aaagccgcaa aaccctttcg gcatcgagcg tttcgaaatc cagaacgctg
     1981 cccatcacgg gcggcagcgc gaaatcgacg acgccgggac cgtgttgacc gggcatcgaa
     2041 gagatgcgca ggcgcgcgtc gcctttcgta aaatcgaccg cttcccaggt gtctagcccg
     2101 attgccttgc gaaaattttt cgcttcgagc tcttcgcgcg cgtgattggt cgtgataatc
     2161 ggcaaatctt tattcagctt ttcttcggcg atgcggtcga aatggtcgcc gtgataatgc
     2221 gacaaaacgc ataaatccag cggcggcaaa tcttcgatgt ccatcgccgg attggtcagg
     2281 cgctccgagg tcagaccgta gccgagatgc acgtggtcgc ccgcgtgcag aaaattcggg
     2341 tcggttaaga ttgtgaaacc ggcgtagcgc aaaatgaccg tcgccgtgcc gataaaaaaa
     2401 atcgaacctt gattgaaatc agcgtcgccc tccgcttcgg acgccgaaag ttgaaatgaa
     2461 ttcgtcatgc aaatctccct tgctcgtcaa aaatatacag ttcggttgct gtataaaaag
     2521 gttatcacaa cggttttgaa acaaacaata tttttccgcg ccgcgcgccc gtctattttc
     2581 ctgaaattcg ccgcgcgccg gttgaatgat tcggtattcc gcgttgccgc cgtttttgac
     2641 tgcggctgac gcttgccggc aagttttttt gaagaatcca gccttaaatt tttgtgtgcc
     2701 gcccttaaag tcatttgtga tagaataatt tgaaaaaaag atgaaaatgt caggaaaagc
     2761 cgcgcggctc gaaaaaatca atcattaaaa tattatgccg accataaaca gaacactccg
     2821 agtcagaacc gacgccgacg ggcgctacga aacgacgcag acttttaatc cgcccggatt
     2881 ttgggattac accattcggg cgtatgcaaa acttattgcg cccgccgaca cgacggtcaa
     2941 cgccagaatc gaaatcagcg ccgccaacca cacgaccgac aatccgccga aagattttcg
     3001 cctttcgacc ggcgaaaccg aagatttagg cagctggcag ctcgacggcg gcgataacac
     3061 cgtcagggtg acgggacaaa cctcgcccgc gcgggcaaac gagatgctcg aaatccaggt
     3121 aacggctgaa atttagaatt atttcccgta aaaacaaccg cgctcaaata atcgtgcgcg
     3181 agcggcgtaa atattgaagc tgttgatttt taataaatca ccggattttc agcctgccgc
     3241 cgtcagctaa aaaagttgac aaaattcggg aaatagattt taatcctttc caagttaaag
     3301 ctttaaagca aaaaataccg acgacaatcg aattcgtccg ggtgaaattt tccgctcgcc
     3361 cgaaaaagcc cgcgaaagtt tcgggctgct tgcgcttcgc ttaaatctac agcctgcggg
     3421 cgcggcggat ttaatagttg gcggttttgt ttcgagccgg gaattaaaaa tttgttttcc
     3481 gcggcgtgcc ggagaagata gttaagacgg cgggtcggca tttctgaaaa atcgacgcaa
     3541 aaaagccgcg ccgcctgctc aaatctttgt ttattatcga ccggcgacat ttaagccagg
     3601 caatgatagg acataattag agggcgtatg aag
//
LOCUS       DASWNE010000937         3992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_209238, whole genome shotgun sequence.
ACCESSION   DASWNE010000937 DASWNE010000000
VERSION     DASWNE010000937.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209238"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..802
                     /locus_tag="VGB68_18305"
     CDS             209..802
                     /locus_tag="VGB68_18305"
                     /inference="COORDINATES: protein motif:HMM:NF044575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051254.1"
                     /translation="MFYPLPDALKLKARPIPRLFLPIKNLPTAHNDVFMKKYDKEEQK
                     LMADWAADCAERVLPFFEKAFPKDNRPRQAIQACRTWVRTGLFKMAEIRGASLAAHAA
                     AREAKAGSAACFAARAAGQAAAAAHVSQHAFGSAYYALKATAAVDLPSAEKNVLEEQE
                     RQVHLIATELRQEVLKRVIVEKRTGGIFIKVQKGDDF"
     gene            complement(836..1681)
                     /locus_tag="VGB68_18310"
     CDS             complement(836..1681)
                     /locus_tag="VGB68_18310"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY0051255.1"
                     /translation="MNSLKKHALFPDSLATAANPSFKVITFGSIKKMRSNQLKRARIK
                     ARLKNVFIWLRALAGSVLFLGLAGAAGALFLFAWLAEEVLEGDTEAFDETIRAFVHGF
                     AGEWLTGFMSFLTILGSTLFLSVVFICVFIVFYLIKWKRDAVLLTVTMVGAVILNFVL
                     KVSFARPRPVPFFDTPLPASYSFPSGHALFAVCFYGALAWLVAARMRERSLKILIWFF
                     AVPIVLLIGLSRIYLGVHYPSDVIAGYAAAIIWVLTVIWTDLLLKKSSFFNKQALKEK
                     IRSLL"
     gene            complement(1828..2811)
                     /locus_tag="VGB68_18315"
     CDS             complement(1828..2811)
                     /locus_tag="VGB68_18315"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEY0051256.1"
                     /translation="MNEQTSINQDKIINTIKPAAALALPLTLIINAASGSSDKADERK
                     ILADFFAEKGIEAEINLARSGAEIVQMAKSAARGASQTIIAGGGDGTISAVAAELVDS
                     DKTLGVLPLGTLNHFAKDLNISLDIKEAAQTIGDGYSVKVDVGEVNGQVFINNSSLGL
                     YPDIVRTREQRQRLGYGKWNSLLRSAFRVFTRYPFLDIRLNAEDKKINTRTPFVFVGN
                     NEYEMESFNIGGRTCLNGGHLSLYMTPHTGRFGLSRMALKALFGRLSQEKDFVSLCID
                     EVLIESRRRQLRVATDGEVSMMETPLHYRIRKGALRVIVPAPVENENVEKK"
     gene            complement(2820..3674)
                     /locus_tag="VGB68_18320"
     CDS             complement(2820..3674)
                     /locus_tag="VGB68_18320"
                     /inference="COORDINATES: protein motif:HMM:NF018436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HEY0051257.1"
                     /translation="MENRSTPLGIIKEQGERVEKSAGLWITLLARFGYAAKGIVYITI
                     GVLAAYAAFTPGGKTTDSRGALEEILFQPYGKYLLGAVALGLAGYALWRFVQAVKDTE
                     NKGSGAKGIALRLGYAAIAIIYIGLALSAVRMILGSGGESTGDSTSQEWTATLLAQPY
                     GQWLVGAVGLGFVAAALSHFYKAYTAKFREKLMTSQMGEKAQSFAHRSGQFGLAARGV
                     VFGLIGAFLIQAALHSNAGEARGLSGALAALGQQTYGQLLLGVVALGLVAYGFYMLVL
                     ARYRRIII"
BASE COUNT         1042 a         1052 c          975 g          923 t
ORIGIN      
        1 gtttgattta acggctcaaa aacgcttcct ttcgggacag tcaaaacgga ttaagatgtc
       61 tctcataaac gattcatttt gagacacata agagcggtaa ttcgcgccta tttcggattt
      121 accagccgct cggcggtata atgaccggtt aggcgtctat cttaaaataa cggtatgaag
      181 aaattaatca tctcaagttt tatatttttt gttttatccg ctaccggatg ctctcaaact
      241 aaaagcccga ccaattcccc ggctgttcct tcccattaag aatctgccga cggctcataa
      301 tgacgttttt atgaaaaaat atgacaagga agagcaaaag ttaatggctg actgggctgc
      361 ggattgtgcc gaaagggtgc tgccgttttt tgagaaagcg ttcccgaaag ataatcgtcc
      421 gcgccaagcc attcaagcgt gtcggacctg ggttcggacc ggcttgttta agatggcaga
      481 gatacgcgga gcgtcacttg cggcgcacgc cgccgcccgc gaagcgaaag ccggcagcgc
      541 ggcttgtttt gccgcacgcg ccgccgggca agctgcggct gcggcgcacg tttcgcaaca
      601 cgccttcggg agcgcgtact atgctcttaa agcgacggca gcggttgatt tgccttccgc
      661 cgaaaagaac gttctcgaag aacaggagcg tcaagtgcat ctcatcgcaa cagagttgcg
      721 gcaagaagtt ttgaaaagag tgatagtcga aaagcgcacg ggcggaatct ttatcaaagt
      781 gcaaaaggga gatgattttt aataaaaaga ccaattttac ttgtccgaat gaaggtcaga
      841 gcagcgagcg aattttttct ttgagagctt gtttgttgaa aaaactgctc ttcttaagca
      901 gcaaatccgt ccatataaca gttaaaaccc aaatgattgc ggcggcgtag cctgctatca
      961 catcgctcgg ataatgaacg cccaaataaa tgcgggagag tccaatcaat agaacaatcg
     1021 gaacagcaaa aaaccaaatt aaaattttca gggagcgttc gcgcatacgc gcggcaacga
     1081 gccaggcgag cgctccataa aagcaaactg caaaaagcgc atgcccgctg ggaaaactgt
     1141 atgaagcggg cagcggcgtg tcaaaaaacg gaaccggtcg agggcgggcg aaacttactt
     1201 tcaacacgaa attaagaatt acggctccga ccatagtcac cgttaataaa acggcatcgc
     1261 gtttccattt gataaggtag aaaactataa aaacacaaat aaaaacgacg ctcagaaaca
     1321 gcgtcgagcc gagtatcgtc aggaaactca taaaaccggt taaccattcg ccggcaaacc
     1381 cgtgaacgaa ggcgcggatg gtttcgtcga aagcctcggt gtcgccctcc agaacttctt
     1441 cagccagcca cgcaaagaga aataacgcgc ccgccgcgcc cgccaagccg agaaacaaaa
     1501 ctgacccggc taaggctcga agccaaataa aaacattttt caagcgtgct ttgattcggg
     1561 cacgttttaa ttgattcgag cgcatctttt ttatcgaacc gaaagttatt accttgaacg
     1621 acggatttgc ggctgtcgca agagaatcgg ggaaaagcgc gtgcttcttt aaactgttca
     1681 tcgtgcgccc gccgcgtcaa atcgccgaaa acggcgacga tattcgggtc gagcccggtt
     1741 atcaaaccga gcaacggctc aataatcgcc gggtcagcgc gtccgaaatg aatatcagac
     1801 aaatgcgcgg gcgttcgcat ttaacaatca cttcttttca acattttcat tttcgaccgg
     1861 cgcgggaacg atcacccgca gcgcgccctt gcgaatccgg tagtgcagcg gcgtttccat
     1921 catcgaaact tcgccgtccg ttgcgacgcg caattgcctg cggcggcttt ctatcaggac
     1981 ttcgtcgata caaagcgaga cgaaatcttt ttcctggctc agcctcccga aaagcgcttt
     2041 cagagccatt cgcgataatc cgaatcgtcc ggtgtgcggc gtcatataca aacttaaatg
     2101 cccgccgttc agacacgttc gcccgccgat attaaaactt tccatttcat attcattatt
     2161 tccaacgaag acgaaaggcg tgcgcgtgtt aattttcttg tcctcggcat taagccgaat
     2221 atcgagaaac ggataacggg taaatactct gaacgccgaa cgaagcagcg aattccattt
     2281 tccgtaaccg agtcgctgtc tttgctcgcg cgtgcgcacg atgtccgggt acagccccag
     2341 actggagtta ttaataaata cctgcccgtt gacttcgccg acgtcgactt ttaccgagta
     2401 accgtcgccg attgtctgtg ccgcttcttt aatgtcgagc gatatgttta agtctttggc
     2461 gaaatggtta agcgttccga gcggcagaac gcccagcgtt ttatccgaat cgactaattc
     2521 agccgccacc gcgctgattg ttccgtcgcc gccgccggcg ataatcgttt ggctggctcc
     2581 gcgcgccgcg cttttagcca tttgcacgat ttccgctccg ctgcgtgcca ggttgatttc
     2641 cgcttcaatt cctttctcgg caaagaaatc ggcgagaatc tttcgttcat ctgctttatc
     2701 gctgctgcct gatgcggcat tgataatgag ggtgagcggc agcgctaaag cagcagccgg
     2761 tttgattgtg tttataattt tatcttgatt aatcgatgtt tgttcattca ttttatttgt
     2821 tagatgataa tgcgtcgata acgcgccagc accaacatat aaaacccgta agcaactaat
     2881 ccgagcgcca ctacgccaag cagcagctgt ccataggtct gctgtccgag cgcagctaaa
     2941 gcgccgctca agccgcgcgc ctcgcccgca ttcgaatgca gcgcggcttg aatcaaaaat
     3001 gccccgataa gtccgaagac cacgccgcgc gccgccagcc cgaattgacc ggaacgatgc
     3061 gcaaagcttt gcgctttttc gcccatttga ctcgtcatca gtttttcgcg gaatttagcc
     3121 gtgtaagcct tgtaaaaatg ggataaagca gcggcgacaa aacctaatcc gacagcgccg
     3181 accagccatt gaccgtaagg ttgagcgagc aaagtcgcgg tccattcctg cgacgtggaa
     3241 tcgcctgtgc tttcgccgcc ggaaccgaga atcatccgta cggcggaaag agccaagccg
     3301 atgtaaataa tcgcgatagc cgcataaccg agccgaaggg caataccttt cgcgccggag
     3361 cctttgttct ccgtgtcctt tacggcttga acaaaacgcc acaacgcgta acccgccaat
     3421 ccgagcgcaa cggcgcccaa taaatattta ccgtaaggct gaaacaggat ttcttctagc
     3481 gcgccgcgcg agtcggtcgt tttgccgccg ggtgtaaaag cggcgtaggc ggcgagaacg
     3541 ccaatcgtaa tataaacaat tcccttcgct gcatagccga acctggcgag cagcgtaatc
     3601 cagagaccgg cggatttctc aacccgctcg ccctgttcct tgatgattcc gagaggcgta
     3661 cttctatttt ccattgatgc ttcctttaac cgaattaatt gacaaatctt actgcgaaaa
     3721 acatcgtgtg ttctcataat aacagcttaa ggaaaataat tcccgacgaa aaaacgcgcg
     3781 acgatgccga accggcttgg cgcagcgtcg tccgcaattt cgtctggaag cctgcggttt
     3841 gcagaaatgc ctcttttcga gacacttcaa ataagcgtct cgaaaagagg catttccggc
     3901 ggatgcagtc aaggattccg tgtcgctttc ctgcaaaatg cctgactaag gcgaagccgt
     3961 gaaatctacg tccgtcagct cgtccataac ag
//
LOCUS       DASWNE010000938         2534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_701240, whole genome shotgun sequence.
ACCESSION   DASWNE010000938 DASWNE010000000
VERSION     DASWNE010000938.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701240"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1021)
                     /gene="ccsA"
                     /locus_tag="VGB68_18325"
     CDS             complement(<1..1021)
                     /gene="ccsA"
                     /locus_tag="VGB68_18325"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEY0051258.1"
                     /translation="MSQVLTANEIRETKGGSSWTGYLPSILVILGAFAMLFLRLEIGA
                     KFISDTALMMLALACYILAALFQLTNLYAPSEMAQRIGLWSGALGVFFNLSSWLVRWV
                     AAYDFEIVQLRAAGSMETPWMFRYIPFANLYDLSLAFAFGAGITTLLLAKRENFQFLS
                     AFTLPLAAIILTLARFIGGEHSNLMPILDSYWRPIHVGVASLSYGVTLVCFAIAVIYL
                     LKDNVKVESMAIWASVFALGVFATISKFSVFTDFVYRAGIFVPGTPKPVSAPFRLEIP
                     YVGLSLVIAGVLCAGMIASFLLYLYKNNEAAKKIGHILLKLSLVAQVLAIAFLVSGIK
                     SATDVN"
     gene            complement(1074..>2534)
                     /locus_tag="VGB68_18330"
     CDS             complement(1074..>2534)
                     /locus_tag="VGB68_18330"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HEY0051259.1"
                     /translation="QPIGNRFLDFLSSVRFGVVLLCIMVFLAFMGMIIIQQNVQGFET
                     YFASLTPAEKWLFGNLGLFDIYHSWYFNFLLLVLSLNIVLASIDRFPGAWKYVSTPKT
                     DASRAWLLNQKFNAKISTHGESPQAVAEKIGEVFRRNGLKARVTKKNNRVYIFGESGK
                     WNRLGAYIVHVALLTLFLGHFVALQSGFDADVRLMPGQKTNEIQLIDFDLDDKQGLQT
                     QRYNVALPFSITCTDIEQKLIDPKKDIGIQNTLDWRTQIKIEDPEFGETVADVQLNKP
                     FSYRGFRFFQASAITVGSARNMTLELAPENGGQPLSVNLARNGSASLADGTKIDYEAF
                     FPDFTLSNGQADTRSPDYNNPAVKLNITTPSGEKKNAYAFFNKLPDGIPIGAPVAGYK
                     FRLADFEKVPLAHVLSIKYDPYHGAFIAWYFGGFGLIGALVFVFFLSHKRVWALIQKK
                     DLSESYEVVLGGNTNRSQQGFEDKFKRIVEKLETQT"
BASE COUNT          692 a          658 c          632 g          552 t
ORIGIN      
        1 cgttgacatc ggtcgccgat ttaatgcccg aaaccaaaaa ggcaatcgcc aacacctgcg
       61 cgacgagcga aagctttaac aaaatatgac cgattttttt cgccgcttcg ttgtttttgt
      121 aaagatagag cagaaagctc gcaatcatac cggcgcacaa aacgcccgca atcaccaggc
      181 ttagaccgac gtaaggaatt tccagacgaa acggcgcgga aacgggtttc ggcgtgccgg
      241 gaacaaaaat tccggcgcga tagacgaaat ccgtaaacac cgaaaatttg ctgatggtcg
      301 caaaaacgcc gagcgcgaaa acgctcgccc aaatcgccat cgactcgacc ttgacgttgt
      361 ctttcaacaa ataaatcacc gcaatggcaa aacaaaccag cgtcacgccg tagctcaaag
      421 acgcgacgcc gacgtgaatc ggtcgccagt aagaatcgag aatcggcatc agattagaat
      481 gctcgccgcc gataaagcgc gccaaagtca aaataatcgc ggcgagcggc aaggtaaacg
      541 cgcttaaaaa ttgaaaattt tcgcgtttgg cgagcaaaag cgtcgtaatg cccgcgccga
      601 aagcgaaagc gagactcaaa tcatacaaat tggcaaacgg aatatagcga aacatccagg
      661 gcgtttccat acttccggcg gcgcgaagct gcacgatttc aaaatcgtag gcggcgaccc
      721 accgcaccag ccagctcgaa agattgaaga aaacgccgag cgcgccgctc cagagcccga
      781 ttctctgcgc catttccgaa ggcgcgtaga gattcgtcag ttgaaacagc gccgctaaga
      841 tatagcaggc gagcgccagc atcatcagag ccgtgtcgga gataaatttc gcgccgattt
      901 ccagacgcag aaacagcatg gcgaacgcgc cgaggatgac taaaatcgac ggcagataac
      961 cggtccacga agaaccgcct ttggtttcac gaatttcgtt ggcagttaaa acttgcgaca
     1021 taattttata agtgctcgat gcaaagcgta aaatacaaat tatttttgaa tcttcaggtt
     1081 tgcgtttcca gtttttcgac gattcttttg aatttgtcct cgaaaccctg ttggctgcgg
     1141 tttgtattgc cgcccaaaac gacctcgtag ctttcgctta aatctttttt ctgaatcaaa
     1201 gcccagacgc gcttgtgcga aagaaaaaac acgaaaacga gcgcgccgat caagccgaaa
     1261 ccgccgaaat accacgcgat aaacgcgccg tgatacgggt cgtatttgat agacaggacg
     1321 tgcgcgagcg gaactttttc aaaatcagcc aggcggaatt tataacccgc gaccggcgcg
     1381 ccgatcggaa ttccgtcggg cagtttatta aaaaaagcgt aggcgttttt cttttcgccc
     1441 gacggcgtcg taatgttcag cttgaccgcc ggattgttgt aatcgggcga gcgcgtgtcg
     1501 gcttgcccgt tggaaagcgt gaaatcggga aaaaacgctt cgtagtcgat tttcgttccg
     1561 tccgcgagac tcgccgagcc gttgcgcgcc agattaacgc ttaaaggctg accgccgttt
     1621 tccggcgcga gctccagcgt catattgcgc gcgctgccga ccgtgatggc gctcgcctga
     1681 aaaaatcgaa agccgcgata gctgaaaggt ttgttcaatt ggacgtcggc gacggtttcg
     1741 ccaaactccg ggtcttcgat ttttatctgc gtccgccagt cgagcgtgtt ttggatgccg
     1801 atgtcttttt tcgggtcaat cagtttttgc tcgatgtcgg tgcaggtaat cgaaaagggc
     1861 agcgcgacat tgtaacgctg cgtctgcaag ccctgtttat cgtccaaatc gaaatcgata
     1921 agctgaatct cgtttgtttt ctgtccgggc atcaatcgca cgtcggcgtc gaaaccgctt
     1981 tgcagcgcga cgaaatgtcc cagaaacaag gtcaaaagcg cgacgtgaac gatatacgcg
     2041 cccaggcgat tccattttcc gctctcgccg aaaatataaa ctcgattgtt ttttttcgta
     2101 acgcgcgctt tcaagccgtt tcggcgaaaa acttcgccga ttttttcggc aaccgcctgc
     2161 ggcgattcgc cgtgcgtcga aattttcgcg ttgaatttct gattcaaaag ccacgcccgc
     2221 gaagcgtccg ttttcggagt cgaaacatat ttccacgcgc ccggaaagcg gtcaatcgaa
     2281 gccagaacga tatttaagga aagaaccaga agcaggaaat tgaaatacca gctgtgataa
     2341 atatcaaaca agccgagatt gccgaaaagc catttttccg ccggcgtcag cgaagcgaaa
     2401 tacgtttcaa agccctgcac gttttgctgg atgattatca ttcccataaa ggctaggaaa
     2461 accataatgc acagcaaaac gacgccgaac cgaaccgagc ttaaaaaatc gagaaaacgg
     2521 tttccgatcg gctg
//
LOCUS       DASWNE010000939         2009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_524873, whole genome shotgun sequence.
ACCESSION   DASWNE010000939 DASWNE010000000
VERSION     DASWNE010000939.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524873"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VGB68_18335"
     CDS             complement(<1..752)
                     /locus_tag="VGB68_18335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HEY0051260.1"
                     /translation="MITVDYPLWAWISFFAIVLTALAVDLGIANRRTHEPTRRETFAW
                     SAVWISLALGFNGFIYWMIYSHFGYNAAAHDLAIIKTKEYFTGYLIELSLSVDNLFVF
                     LLIFNYFKVPKKYQHRVLFWGIFGALVMRMTMIFAGAELVERFHWIIYIFGAFLVYTG
                     IKMFGGDDEDFHPEKSPLVSFITRFVRISKHYDKEKFIIIKDGKRTGTLLLLVLIVVE
                     VSDLIFAVDSIPAIFGISTDKFIVYTSNVFAIL"
     gene            1121..>2009
                     /locus_tag="VGB68_18340"
     CDS             1121..>2009
                     /locus_tag="VGB68_18340"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEY0051261.1"
                     /translation="MLKFFSRLEKTRNVLILIFGLLLVFSMVFFGASIIGTGNNTAVS
                     TGSTETVAKVGSESVTVGELIRQKENLSQMYGGRAPASKLLLDGMISQRIVRQEAERL
                     GLTASDAEVAASIREQNKPQDGKPLDIARYEQNVTEQYGSVPAYEETVRDSVSGQKVR
                     AFITSGVTVSEQEVLDDFKKRNTKFDVSYVPVSVADLAQTIKPTDAELQDFFNRNKGN
                     YYISEPQKKIRYIFLSTSKIGEKTSIPEADLKAEYEKLPEDKRNSGVQAQQIVLRIAK
                     PEFDAQVLEKANQIVERARS"
BASE COUNT          598 a          516 c          483 g          412 t
ORIGIN      
        1 agaatagcaa aaacattcga tgtatagaca ataaatttat cggtcgagat gccgaaaatc
       61 gccggaatcg aatcgaccgc gaaaatcaaa tccgacactt cgacgacgat taaaaccagc
      121 agcagcaaag ttcccgtccg cttgccgtcc ttaataataa taaatttttc tttgtcgtaa
      181 tgtttcgaaa tacgcacaaa ccgcgtgata aaggaaacca gaggactttt ttccgggtga
      241 aaatcttcat cgtcgccgcc gaacattttt attccggtgt aaaccagaaa cgcgccgaaa
      301 atataaatta tccagtgaaa ccgctcgacc agttccgcgc ccgcgaaaat catcgtcata
      361 cgcatcacca gcgcgccgaa gatgccccag aaaagaacgc ggtgctggta ttttttcggg
      421 actttaaaat aattgaaaat cagcagaaag acaaaaagat tatcgaccga cagcgaaagc
      481 tcgattaaat aacccgtgaa atattccttg gttttgatta tcgccaaatc gtgcgcggcg
      541 gcgttgtaac cgaaatggct gtaaatcatc caataaataa aaccgttaaa accgagcgcc
      601 agcgaaatcc agaccgcgct ccacgcaaag gtttcacggc gtgtcggctc gtgcgtgcgg
      661 cggttggcga tgcccaaatc gacggcgagc gccgtcagca cgatagcgaa aaaagaaatc
      721 caagcccaaa gcgggtaatc aacagtaatc ataaacagat aagatacaag cctcgaaagg
      781 caaaagtaaa aagtaaaaag gcaaaaaacg aattccaccc agcccgtctg ccgggcggcg
      841 gcaaaatttt taaatctccg acaaccgccg ccgcgcaaaa gacaactaaa tactttggac
      901 attttacgat ttatgccgcg taatgttaaa atcgcggttt gctagaaaag gcagaaacgt
      961 gcccgcggcg gcgagcgccg ttaattcaaa actcaaaatt caaaattata ttttgcgctt
     1021 tgcgttttcc gtttcgcagt tttcccgaga gccgcagcgc gccgccgcaa aacgtttctg
     1081 tccggtccga ttccaatttt cttaaaacag gtagtaaaca atgcttaagt ttttcagtcg
     1141 tttagaaaaa accagaaatg tcctgatttt gattttcggg cttctgctgg ttttcagtat
     1201 ggtctttttc ggcgcgtcga tcatcggcac cggaaataac acggcagtct cgaccggcag
     1261 caccgaaacc gtcgccaaag tcggcagcga atcggtgacg gtcggcgagc ttatccggca
     1321 gaaagaaaac ctcagccaga tgtacggcgg gcgcgcgccg gcgagcaagc ttttgctgga
     1381 cggaatgatt agccagcgca tcgtccggca ggaagccgaa cggctcggct taacggcgtc
     1441 cgacgcggaa gtcgccgctt ccatccgcga acaaaacaaa cctcaggacg gcaagccgct
     1501 cgatatagct cgctacgaac agaacgtgac cgagcaatac ggcagcgtcc cggcttacga
     1561 agaaaccgtc cgcgacagcg tcagcgggca gaaggtgcgc gcctttatca cgtcgggcgt
     1621 gacggtttcc gaacaggaag ttctggacga ttttaaaaaa cgcaacacca aattcgacgt
     1681 gagctatgtg ccggtcagcg tcgccgatct ggcgcagacc atcaagccga ccgatgccga
     1741 attacaggat tttttcaatc gaaacaaagg caattactat atttcggagc cgcagaaaaa
     1801 gattcgctac attttcctga gcacttccaa aatcggcgaa aagacttcga ttcccgaagc
     1861 cgatttgaaa gccgagtacg agaaactgcc ggaagacaaa cgaaactccg gcgtgcaggc
     1921 gcagcagatt gttttgcgca ttgccaagcc ggaatttgac gcgcaggtct tggaaaaagc
     1981 caatcaaatc gtcgaacgcg cccgcagcg
//
LOCUS       DASWNE010000940         3256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_490285, whole genome shotgun sequence.
ACCESSION   DASWNE010000940 DASWNE010000000
VERSION     DASWNE010000940.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490285"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..1272)
                     /locus_tag="VGB68_18345"
     CDS             complement(208..1272)
                     /locus_tag="VGB68_18345"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051262.1"
                     /translation="MGNKKTKAIFDTVLARRKIEQGRRAKWWRRVGGKSKGFAYLNAD
                     GKRVTEESHLARIKSLVIPPAWKYVRISPAASSRIQALGVDTSGRIQYLYHSRFAEKQ
                     QKKKFEKIERFGEYLPRLRKLTNEHIALEGFPREKVLAVVLRLINSLYIRVGTEKSVR
                     HYKTYGITTLQNRHLEIGENGELVFSFVGKHHIRHRKVLVDEELSGVMRDLKALGSAR
                     KLFHYLDADGKPRPIKPKDVNDYLKTATAPEFSAKDFRTWGGSLLTAVELAEIGTAEE
                     EKQIKQNICKAVKKVAEQLGNTPTVCRGSYIHPTIIKSYESGVTIDEFRPRKTRRINR
                     IESDYEPEEKALIKMFREGK"
     gene            1468..1977
                     /locus_tag="VGB68_18350"
     CDS             1468..1977
                     /locus_tag="VGB68_18350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051263.1"
                     /translation="MTLETTDNIVEFENVEAAQSKKDMILSLFKAGTTDIESIAVLSG
                     AKPSYVGSVLQREGLIDNYFDLYTSTAHSMNVYSKYFRGKLGFKNVKAAEGGVINLEQ
                     AYRLFSNAQDRAGQHHTLEMALTMLNRARWTGKLAEAEVYRRWLVGKLSQPLVEEKEN
                     TQQVLKLVA"
     gene            2100..3155
                     /gene="ruvB"
                     /locus_tag="VGB68_18355"
     CDS             2100..3155
                     /gene="ruvB"
                     /locus_tag="VGB68_18355"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF000868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEY0051264.1"
                     /translation="MALELVESPLRQNGLIVGEATIEDVSAELSLRPKKLDEYIGQTK
                     IVNNLRIFLKAALRRSEALDHVLLFGPPGLGKTSLAIILANELGASLKTVHAPSVDKS
                     GDLAAILTNLEEGDVLFIDEIHRLRPQIEEILYPAMEDYSLDLMIGQGTAARSIKIDL
                     PKFTLVGATTRAGMISAPLRGRFGIVNHLDFYPKKDLEIIIHRSAEILNVEIEATGAS
                     ELAKRARGTPRIANRLLRRVRDYAQVLGDGRITGALAQKGLDAMEVDRFGLDEVDRKL
                     LLTIIEKFDGGPVGVGTIAASISEDRESIEEVIEPYLIQIGFLNRTPRGRTVTGAAYK
                     HFGYKPPVMQEVQAMAS"
BASE COUNT          810 a          761 c          839 g          846 t
ORIGIN      
        1 atcgcatcga atcgaatgcc gattatgaac cggaagaaaa agctctgata aagatgtttc
       61 gggaaaacgg taaaaagtgg taaacggtaa aaagtggtaa gcggtaaacg gtaaacggta
      121 agttgcaatt gttgagcatc gagtttttat ttaaaacttg aaattctaac aacgaactta
      181 ccgtttaccg tttaccgctt accactttta tttaccttcc cgaaacatct tgattaaagc
      241 tttttcttcc ggttcataat cggattcgat tcgattgatg cggcgcgtct ttcgcgggcg
      301 gaattcatct atcgtgacgc ccgattcgta agattttatg atggtcggat gaatatacga
      361 gccgcgacag acggtcggcg tgttgccgag ctgctcggcg acttttttca ccgctttaca
      421 gatattttgc ttgatttgtt tttcttcttc ggcagttccg atttccgcca gctcgaccgc
      481 cgtcagaagg cttccgcccc aggtgcgaaa atccttcgcc gaaaattccg gcgcggtcgc
      541 cgttttcaga taatcgttga cgtctttcgg tttaatcggt ctcggcttgc cgtccgcgtc
      601 cagataatga aaaagcttgc gcgccgagcc gagcgctttt aaatcgcgca tcacgcccga
      661 caattcttcg tcaaccagaa cctttcggtg ccggatatga tgcttgccga cgaagctgaa
      721 aaccagctcg ccgttttcgc cgatttccag atgacgattt tgcagcgtcg tgatgccgta
      781 agttttgtaa tgcctgacgc ttttttccgt tccgacgcga atatacagcg aatttatcag
      841 gcgcaaaacg acggctagaa ccttctcgcg cggaaagcct tcgagcgcga tgtgctcgtt
      901 cgtcagcttt ctcaatcgcg gcaggtattc gccgaatctt tcgattttct caaacttttt
      961 cttctgttgt ttttctgcga atctcgaatg gtaaagatat tggattctgc cggaagtgtc
     1021 cacgccgagc gcctgtatcc tgctgcttgc ggcgggcgaa atccggacgt atttccaggc
     1081 gggcggaatg acgagagatt tgattcttgc caggtgagat tcttccgtga cccgctttcc
     1141 gtcggcattc agatacgcga aacctttcga ttttccgccg actctgcgcc accatttcgc
     1201 gcgcctgccc tgttcgattt ttcgtctggc aaggactgtg tcaaaaatcg ctttcgtctt
     1261 cttgtttccc atcgagccca accctttttc gttcttttta atgagtataa cttattattt
     1321 tcgttattgg aaaaataatt tcggtgcgcc cgcaaatgaa gaaatgatta ggtttaattt
     1381 ggcaaattta tctcgcgaaa atacttgaaa aggcaacttg tttcatctat gaaacatcaa
     1441 atataatgtt aaatggaggg tagaaaaatg actttagaaa cgaccgataa tattgttgaa
     1501 tttgaaaacg tcgaagccgc gcaaagcaaa aaagatatga ttttatcgct tttcaaagcg
     1561 ggcacgaccg atatcgaatc aatcgccgtt ttaagcggcg ccaagccgag ctatgtcggt
     1621 tcggttttgc agagagaagg tttgattgat aattattttg acctttatac ttcgaccgca
     1681 cattcgatga atgtctattc caagtatttt cgcggaaagc tcggttttaa gaatgtcaaa
     1741 gcggcggaag gcggagtgat taatctggaa caggcgtatc gcttgttttc aaacgcgcag
     1801 gaccgcgccg gacagcatca cacgctggaa atggctctga cgatgctcaa ccgcgcgcgc
     1861 tggacaggca agctggcgga agccgaagtt tatcgacgct ggctggtcgg caaattgagc
     1921 cagccgctgg tcgaggaaaa ggaaaacacg cagcaggttt taaagctcgt ggcatagttt
     1981 gcggcttgtg tttaaatttg caagccgcaa atttttcggc gtcttgtttc acagttgaaa
     2041 caatcttgca taaagagtaa aatagacagg taataaaaaa caaaaaagag gtttctcaaa
     2101 tggcattgga attagtggaa agtccgctca ggcagaacgg attaattgtc ggcgaggcaa
     2161 ccatcgaaga cgtttcggcg gagctcagtt tacgtccaaa aaagctcgac gaatacatcg
     2221 ggcagacgaa aatcgtcaat aatctcagga tttttttaaa agccgccctg cgccgcagcg
     2281 aagcgctcga ccacgttctg cttttcggtc ctccgggatt gggcaaaacg agtctggcga
     2341 taattctggc gaacgagctt ggcgcgagcc tgaaaacggt tcacgcgccg tcggtcgata
     2401 aatcgggcga tttggcggcg attctgacga atctggaaga aggcgacgtt ttgtttatcg
     2461 acgaaattca ccgcctgcgc ccgcaaatcg aagaaattct ctatccggcg atggaagatt
     2521 acagtctgga tttgatgatc gggcagggca cggcggcgcg ctcgataaaa atcgatttgc
     2581 cgaaatttac attggtcggc gcgacgacgc gagccggaat gatttccgcg cctctgcgcg
     2641 ggcgtttcgg aattgtgaat cacctggatt tttatccgaa aaaagatttg gaaatcatca
     2701 ttcatcgctc ggctgaaatt ctgaacgtcg aaatcgaagc gaccggcgcg tcggaattgg
     2761 caaaacgcgc gcgcggaact ccgcgcatcg ccaaccgcct gctgcgccgc gtgcgtgatt
     2821 acgcgcaggt tttgggcgac gggcgcatta cgggcgcgct ggctcagaaa ggcttggacg
     2881 cgatggaagt cgaccgtttc ggtctggacg aagtcgatag aaagctgctt ctgacgatta
     2941 tcgaaaaatt tgacggcggt ccggtcggcg tcggcacgat tgccgcatcc atcagcgaag
     3001 accgcgagtc aatcgaagaa gttatcgagc cttacctgat tcagatcggt tttctgaacc
     3061 gcacgccgcg cgggcgcacg gtgacgggcg cggcttataa acatttcggt tataaaccgc
     3121 cggtaatgca ggaagtgcag gcgatggcga gctaaagtag aagacagaag ccggaagccg
     3181 gtagtcagta gaattttgaa attcaatgat gaatattcaa atctaattct gactaccggc
     3241 ttccggcttc cggcgt
//
LOCUS       DASWNE010000941         2991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_413924, whole genome shotgun sequence.
ACCESSION   DASWNE010000941 DASWNE010000000
VERSION     DASWNE010000941.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413924"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VGB68_18360"
     CDS             <1..328
                     /locus_tag="VGB68_18360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051265.1"
                     /translation="STEGGDKFWALPAAEIEKFGENWLITEFYFDADWKTWEKHPHGE
                     EIVYLLSGAMDLILDKDGARRTIELRSKGLVVVPRDTWHTAKVFEPSNVLVITHGKQT
                     EIREAE"
     gene            351..617
                     /locus_tag="VGB68_18365"
     CDS             351..617
                     /locus_tag="VGB68_18365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051266.1"
                     /translation="MQYAWYDVLGSIGVGLIILTYIALQLEKIRSEQLIYSLLNAVGA
                     GLIVLSLCFSFNFSAFVVEFFWVLISLYGVGKYFMKGKRREVKK"
     gene            705..1772
                     /locus_tag="VGB68_18370"
     CDS             705..1772
                     /locus_tag="VGB68_18370"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEY0051267.1"
                     /translation="MTVKTSLKKSNNEPLSCTRETSLTKEQILELYRFVKMTRMFDEI
                     TVRLKRQAKLTGGVFTSLGQEATAVGTAYALDEQDFIAPLIRDIGACFVKGISPRTIF
                     AQYLGKANAPSRAADVQFHFADLEKGFVGPISHLGDMIPVMTGILLASRMKNETRVAV
                     AYLGEGASSTGTFHEGVNFAAVRKLPLITVIENNGYAYSTPTRSQCAAEHFVDKAIGY
                     GILGLSVDGNDIVACYETMKKAVEHARGGNGSVLIEAMTYRRKGHAEHDNQSYVPAGE
                     IDFWAQNNDPIDRFERFLTTEKIASEEELNEAVAKVSAYLEAELAIAEEAGAPEAISA
                     AYEVFDNRIVAPAFKKKVLEK"
     gene            1773..2297
                     /locus_tag="VGB68_18375"
     CDS             1773..2297
                     /locus_tag="VGB68_18375"
                     /inference="COORDINATES: protein motif:HMM:NF015317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HEY0051268.1"
                     /translation="MEWLVEKSRYLPLIGIFSMLVGSMAAFAVGAVKTIHVLQTAVTQ
                     YTEAEPTLYVLFEALDCFLVGTGLIVIAVGLYELFIGGLEVPDWMLVKSLDELKAKFV
                     FVIIPVMAAKFIQKILKQDSNAIETLYYGTAIAIVTLALSAFVFVSIKEKEVDREHSG
                     EEEERRAKDLIKNK"
     gene            2322..2900
                     /locus_tag="VGB68_18380"
     CDS             2322..2900
                     /locus_tag="VGB68_18380"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0051269.1"
                     /translation="MDFSNNGQGNFNNQDSLNNLMPDRSELECEELFAQADRLLNEGV
                     IIDAVEKLSQIVKRNPHFGKAYNHLGWLYETKYKNYARAEEYYKAAMQYAPHYNASYL
                     NYAYFLSNLGRFDELKAHLDRVAQIPGIAKDTVANEYAIMYEMQGNLQQAVDYYQKAA
                     IVTLDSAKLDKYKEGIDRCRKKLDILNPSNSF"
BASE COUNT          786 a          708 c          795 g          702 t
ORIGIN      
        1 atcgaccgaa ggcggcgata aattctgggc gctgcccgcc gccgaaatcg aaaaattcgg
       61 cgaaaactgg ctcatcaccg aattttattt tgacgccgac tggaagacct gggaaaaaca
      121 tccgcacggc gaagaaatcg tctatctgct ttcgggcgcg atggatttga ttctggacaa
      181 agatggagcg cgccggacga ttgagcttcg ctcgaaaggc ttggtcgtcg ttccgcgcga
      241 tacgtggcac acggcgaaag tttttgagcc gagcaatgtg ctggtgataa cgcacggcaa
      301 gcagaccgag attcgggaag cggaataaat ttttatctct ttttatattt atgcaatacg
      361 cctggtatga cgtgctcggc tcgatcggcg tcgggctgat aattctcaca tatatcgcgc
      421 tccagctcga aaaaatccgc agcgaacaac tgatttattc tttgctgaac gcggtcggcg
      481 ccggtctgat agtgctttcg ctgtgtttta gttttaattt ttccgctttc gtagttgaat
      541 ttttctgggt tttgattagc ctatatggag taggcaaata ttttatgaaa ggcaaaagga
      601 gagaagtgaa aaagtgaaaa actgaaaagg taaaaaggtt ttgttgaatt atcgctcgac
      661 ccgccttttt cactctttcg ctttttcact ttttcacttt aacaatgact gtcaaaacga
      721 gtctgaaaaa atcaaataac gagccgcttt cctgcacgcg ggaaacttcg ctgacgaaag
      781 agcagattct ggagctttac cgctttgtga aaatgacgcg gatgtttgac gaaatcaccg
      841 ttcggctcaa acgccaggcg aaattgacgg gcggagtttt tacgtctctg ggacaggaag
      901 cgacggcggt cgggacggct tacgcgctgg acgagcagga ttttatcgcg cccttaatcc
      961 gcgacatcgg cgcgtgtttc gtcaaaggca tcagcccgcg cacgattttc gcgcagtatc
     1021 tcggcaaagc caatgcgcct tcgcgcgccg ccgacgtgca gtttcatttt gccgatttgg
     1081 agaaaggttt tgtcggtccg atttcgcatc tcggcgatat gattccggtg atgaccggaa
     1141 ttttgctggc atcgcgtatg aaaaacgaaa ccagagtggc ggtcgcctat ctcggcgaag
     1201 gggcgagctc gaccggaacg tttcacgaag gcgtcaactt tgccgccgtc cggaaattgc
     1261 cgctgattac cgttatcgaa aacaacggct acgcttattc gacgccgacg cgcagccagt
     1321 gcgccgccga gcattttgtc gataaggcaa tcgggtacgg gattttgggc ttgtcggttg
     1381 acggaaacga catcgtcgcc tgttatgaaa cgatgaaaaa agcggtcgag cacgcgcgcg
     1441 gcggaaacgg ctcggtttta atcgaagcga tgacttaccg ccgcaaagga cacgccgaac
     1501 acgacaatca atcttacgtt ccggcgggcg aaattgattt ctgggcgcag aacaatgacc
     1561 cgatcgaccg tttcgagaga tttctgacga ccgaaaaaat cgcttccgaa gaagaattaa
     1621 acgaagcggt cgccaaagtt tccgcgtatc tcgaagcgga gctggcgatt gccgaagaag
     1681 ccggcgcgcc cgaagcgatt tcggcggctt acgaggtttt tgataatcga atcgtcgcgc
     1741 ctgcgttcaa aaagaaagtg ctggaaaaat aaatggaatg gctcgttgaa aaatcaagat
     1801 atctgccgct catcggtatt ttttcgatgc tcgtcggctc gatggcggct tttgcggtcg
     1861 gcgcggtgaa aacgattcac gttttgcaaa ccgccgttac gcaatacacg gaagccgagc
     1921 cgacgcttta cgttttgttc gaggcgctcg actgttttct ggtcggcacg ggtttgattg
     1981 tcatcgccgt cggattgtac gagcttttta tcggcggctt ggaagttccc gactggatgc
     2041 tggtcaaaag cctggacgag ctgaaagcca aattcgtatt cgtgataatt ccggtgatgg
     2101 cggcgaaatt catccagaaa attttgaaac aggacagcaa tgcgattgag acgctttatt
     2161 acggcacggc gatagcgata gtcacgctgg cgctgtcggc atttgtcttc gtcagcatca
     2221 aagaaaaaga agtcgacaga gagcattccg gcgaagaaga agaaaggcgc gccaaagatt
     2281 tgataaagaa taaatgaagt aaataaacgg aggtgaaatt tatggatttt tccaacaacg
     2341 gacagggaaa ctttaacaat caagattcgc tgaacaattt aatgcccgac cgaagcgagc
     2401 tcgaatgcga agaactgttt gcgcaagccg accgcctgct caacgaaggc gtgattatcg
     2461 acgcggtcga aaagctctcg caaatcgtca agcgcaaccc gcatttcggc aaagcttaca
     2521 atcatctcgg ctggctctac gaaacgaaat ataaaaacta cgcgcgcgcc gaagaatatt
     2581 acaaagccgc aatgcagtat gcgccgcatt acaacgcttc ttacttgaat tacgcttatt
     2641 ttctctctaa tctcgggcgt ttcgacgagc tgaaggcgca tctcgaccgg gtggcgcaga
     2701 ttccgggcat tgccaaagac accgtcgcca acgaatacgc cataatgtat gaaatgcagg
     2761 gcaatctcca gcaggcggtc gattactatc agaaagcggc gatcgtcacg ctggacagcg
     2821 cgaaactgga caaatataag gaaggcatcg accgctgccg caagaagctg gatattctga
     2881 atccgtcaaa cagtttttaa tcagtgctga gtgctaggtg ctgagtgccg agtgaaatat
     2941 aaaactcagc actcagcact cagcactcag cgcttaacac tatgcaaagc t
//
LOCUS       DASWNE010000942         2193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_525029, whole genome shotgun sequence.
ACCESSION   DASWNE010000942 DASWNE010000000
VERSION     DASWNE010000942.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525029"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VGB68_18385"
     CDS             <1..627
                     /locus_tag="VGB68_18385"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEY0051270.1"
                     /translation="QANIFDIPIAKISDEYLKYIRLMKSLDISVAGDFLVMAATLIEI
                     KSKMLLPREPSEENVEEMEDPRRELVNRLLEHQKFKNAAEMLWSRATVEQAVFVRGKI
                     ESDENNAETNATVFDLLSVFQKILARQKQEIELEIEREEMSLAQMLDNLKKQIFKAKE
                     INLREFFEQMRSKTELVLAFIAVLEIVRAESVTLVQERIFGDIILRKT"
     gene            747..1316
                     /gene="scpB"
                     /locus_tag="VGB68_18390"
     CDS             747..1316
                     /gene="scpB"
                     /locus_tag="VGB68_18390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEY0051271.1"
                     /translation="MTEEIDEIEKQPRAIAELMAICEALIFVSDEPIAVKTMADVLEE
                     ERESIQAAVEELANEYEQREGGLQLREIGGGWQISTRAEYHEHVRAFLKTRPSAKLSL
                     AALETIAVIAYKQPVTVPEILEIRGVQSASAIKTLLDKRLIVAKGRKETVGRPMQYGT
                     SKDFLIQFGLKDLSELPSIEDFEDLTQGI"
     gene            1318..1851
                     /locus_tag="VGB68_18395"
     CDS             1318..1851
                     /locus_tag="VGB68_18395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051272.1"
                     /translation="MRVKQSPNELIIQETPGCFWIFGSLFCLVGGLFVYGALGGFSNH
                     DAVSVWTLILTFFMGAIAFAVGFWVIFHAPVTKVRLDRLTETVTLERYGLAGKRKIVY
                     NFQEIKQFFLIEEKDDEDNLIWWLAMDLTGGEKVKISSMPSRSEDDKRSLVFETNQFM
                     YKQMPSHRDVLELEDES"
     gene            1884..>2193
                     /locus_tag="VGB68_18400"
     CDS             1884..>2193
                     /locus_tag="VGB68_18400"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HEY0051273.1"
                     /translation="MQERLQKLIAQAGIASRRRAEELIEAGLVTVNGEIVRELGTKAD
                     PETDHIKVNGKLINKLLEKRESVYVLLNKPKGYLSSAADPEGRKLVTDLVKGFGKLFP
                     V"
BASE COUNT          605 a          523 c          558 g          507 t
ORIGIN      
        1 caggcgaata ttttcgacat cccgattgcg aaaatctcgg acgagtatct gaaatacatt
       61 cgtctgatga aaagcctcga tatttccgtc gccggtgatt ttctggtgat ggcggcgact
      121 ttgatcgaga taaaatcgaa aatgcttctg ccgcgcgagc cttccgaaga aaacgtcgaa
      181 gaaatggaag acccgcgccg cgagttggtc aatcggctgc tcgaacacca gaaatttaaa
      241 aacgcggctg aaatgctctg gtcgcgcgcg acggtcgagc aagccgtttt cgtccgcgga
      301 aagattgaat ccgacgaaaa caacgccgaa acaaacgcca cggttttcga tttactaagc
      361 gtttttcaaa aaattctggc gcggcagaaa caggaaatcg agctggaaat cgagcgcgaa
      421 gaaatgtcgc tggcgcaaat gctggacaat ctgaaaaagc agattttcaa agcgaaagaa
      481 ataaatctgc gcgaattttt cgagcagatg cgctcgaaga ccgagctggt tttagcgttt
      541 atcgccgtgc tggaaatcgt ccgcgcggaa agcgtcactc tcgtgcagga aaggattttc
      601 ggcgacatta ttttaaggaa aacataaggg aaaaacagga cgctgaattt tttaaaatca
      661 agtaagattg attgagattt tatttaaaat tatcctgact aatcctaaat aatcttattc
      721 aatcggcgtt tcgtttcttt ttagcaatga cagaagaaat cgacgaaatc gaaaaacaac
      781 cgcgcgccat cgccgaatta atggcgattt gcgaggcttt aatctttgtt tccgacgagc
      841 cgatcgccgt caagacgatg gctgacgttc tggaagaaga gcgcgaatcg attcaagccg
      901 ccgtcgaaga actggcaaac gaatacgagc agcgcgaagg cggcttgcag ctgcgcgaaa
      961 tcggcggcgg ctggcaaatc tcgacgcgcg ccgaatatca cgaacacgtt cgcgcctttc
     1021 tgaaaacgcg tccgagcgcc aagctttcgc tcgccgcttt ggaaacaatc gccgtcatcg
     1081 cgtataaaca gcccgtcacc gttcccgaaa ttctggaaat tcgcggcgtt caatcggctt
     1141 cggcaatcaa aactcttctg gataaacgcc tgattgtcgc caaaggtcgc aaggaaaccg
     1201 tcgggcgacc gatgcaatac ggcacgtcga aggattttct gattcaattc ggcttgaagg
     1261 atttgtcgga attgccgagc atcgaggatt tcgaggattt aacgcaggga atttaaaatg
     1321 cgcgtcaaac agtcgccgaa cgagctgatt attcaggaaa cgcccggttg tttttggatt
     1381 ttcggctcgc ttttctgtct ggtcggcgga cttttcgttt acggcgcgct cggcggcttc
     1441 tccaatcacg acgcggtatc cgtgtggacg ctcatcttga ctttttttat gggcgcgatt
     1501 gctttcgccg tcggtttctg ggtgattttt cacgcgcccg tgacgaaagt ccgtttagac
     1561 cgcctgaccg aaaccgtcac gctcgaaaga tacggcttag ccggaaagcg gaaaatcgtc
     1621 tataactttc aagagataaa acaatttttt ctaatcgaag aaaaagacga cgaggacaat
     1681 ttgatttggt ggctggcgat ggatttgacc ggcggcgaaa aggtgaaaat ttcctcgatg
     1741 ccgagccgct ccgaagacga caaacgcagc ctcgtttttg aaacgaatca atttatgtat
     1801 aaacaaatgc cctcgcaccg cgatgttttg gaattggaag atgaaagctg agcgaaaata
     1861 agttagaatt tttgtttaac tttatgcagg aacgtttaca aaaacttatc gcgcaagccg
     1921 gaatcgcttc gcgtcgccgc gccgaagaat taattgaagc cggactcgtc accgtcaacg
     1981 gcgaaatcgt ccgcgagctc ggcacgaaag ccgaccccga aaccgaccac atcaaggtca
     2041 acggcaaact gataaataaa ctgctcgaaa agcgcgagag cgtctacgtt ttattgaaca
     2101 aaccgaaagg ctatctgtcc agcgccgccg acccggaagg cagaaagctc gtgaccgatc
     2161 tggtcaaagg cttcggcaag cttttcccgg tcg
//
LOCUS       DASWNE010000943         2436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_31027, whole genome shotgun sequence.
ACCESSION   DASWNE010000943 DASWNE010000000
VERSION     DASWNE010000943.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31027"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VGB68_18405"
     CDS             complement(<1..666)
                     /locus_tag="VGB68_18405"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0051274.1"
                     /translation="MKKALTLILFLLSANLTLSAATTVWDYDGDGKTDLFVLRQVNDE
                     NQWLILKSRDGFFSTTFGRYEPNVYGDGPIPGDFDGDGKYDIAVARGYVETPYRHFII
                     LNSGSNTLTFAQWGLRSDEIMLQDYDGDGKTDFGVYRGGWWYISNSSDGSFRAEKFGG
                     EPLGRRDEPIHGDFDGDGKADLAVITWFPSPFPPMPITFYIKLSTNGTWFSYQMGDKM
                     RDTS"
     gene            complement(987..1898)
                     /locus_tag="VGB68_18410"
     CDS             complement(987..1898)
                     /locus_tag="VGB68_18410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEY0051275.1"
                     /translation="MRKYEFYQLDVFTDKAFAGNPLAVFPEAGELSAEEMQKIAREMN
                     LSETVFVLPSERALRRLRIFTPMRELPLAGHPVVGTWNLLATLGVVPSQAEGTVTIEQ
                     ELNLGILPVDIEYRDEKPLQVTMTQGAFEAGAIISDEREISRLAQGLGLNLAELNFSA
                     GLPVQVVSTGIKALGVPVVSLDALGRCRINSSLLSEIYLSCGAIGCYAFAFETMEDAS
                     RVHARFFAPDDNIPEDPATGSAAGSLAGYLVYHDAIDTKKFTIEQGDFMKRPSRIHAE
                     VTGEKGSVGRVRIGGQSVVAAKGEVYV"
BASE COUNT          643 a          604 c          556 g          633 t
ORIGIN      
        1 cgaggtgtct ctcattttgt cgcccatctg ataactaaac caagtcccgt tcgtgctgag
       61 tttgatataa aaagttatag gcatcggggg aaacggtgac ggaaaccagg tgataacggc
      121 aaggtcggct ttaccgtcgc cgtcaaagtc gccgtgaatc ggctcgtcgc gcctgccgag
      181 cggttcgccg ccgaattttt ccgctcgaaa actaccgtcg gagctgttgc tgatatacca
      241 ccagccgccg cggtaaactc cgaaatcggt cttgccgtcg ccgtcgtaat cctgcagcat
      301 tatttcgtcc gacctcagcc cccattgggc gaaagtcaga gtgttgctgc cgctgttgag
      361 aatgatgaaa tgcctgtagg gagtttcgac gtaacctctg gcgacggcga tgtcgtattt
      421 gccgtcgccg tcgaagtcgc cgggaatcgg tccgtctcca taaacgttag gctcgtatct
      481 gccgaatgtc gtgctgaaaa atccgtctct gcttttcagg ataagccatt gattttcgtc
      541 gttaacctgc ctgagcacaa acaaatccgt tttgccgtcg ccgtcataat cccagaccgt
      601 agtcgcggct gacagcgtta agtttgccga cagcaaaaat aaaattaagg tgagtgcctt
      661 tttcataact gatttccggc ttctgagggc gaaaaaacat aaaacttctt tttcaatcgt
      721 attgccgcaa ggctttgcac aaaaataaag aaagggaaaa ttgtaaattg taaattgtaa
      781 attctaaatt gatgttcggt atcttttgtt aaacaattta gaattaataa ttgagaattt
      841 acaattagcc atcggaattt ttctcgattt tctttatttt tgtgcaaagc gatgccgcga
      901 atcttagcaa aggtatttcg ggaaagcaat tttttccgcc gaacttataa aaaataagat
      961 ttaacttttt aataatttcc gacgctttaa acgtaaactt cgcctttcgc cgcgacgacc
     1021 gattgcccgc cgattctgac tcgtccgacg cttcctttct cgcccgtcac ttcggcgtga
     1081 atccggctcg gacgcttcat aaagtcgccc tgttcaatcg taaatttttt tgtgtcgatt
     1141 gcgtcgtgat aaaccagata accggcaagc gagccagcgg cgctgccggt cgccgggtct
     1201 tcgggaatat tgtcgtcggg cgcaaaaaat cgggcgtgaa cgcgtgaggc gtcttccatc
     1261 gtctcaaacg caaacgcgta acagccgatt gcgccgcagg aaagataaat ttccgaaagc
     1321 agcgacgaat taattcggca acgccccaga gcatcgagcg aaacgaccgg aacgcccagc
     1381 gctttgatgc ccgtcgaaac gacctgaacg ggcaaaccgg cgctgaaatt caactccgcc
     1441 aaattcagtc ccaagccttg cgcgagcctc gaaatctctc tttcgtcgga aataattgcg
     1501 cccgcttcga acgcgccctg cgtcatcgtc acctgtaaag gtttttcgtc gcggtattcg
     1561 atatcgaccg gtaaaattcc cagattcaat tcctgctcaa tcgtcactgt tccttccgcc
     1621 tgagagggaa cgacgccgag cgtcgccaaa agattccagg tcccgaccac cgggtgaccg
     1681 gcgagcggca attcccgcat cggcgtaaaa attctcagac gacgcagagc ccgctccgaa
     1741 ggcaaaacaa aaaccgtctc gctcagattc atctctcgcg cgattttctg catttcttcc
     1801 gcagacagct cgccggcttc gggaaaaacc gccaaaggat tgcctgcaaa cgctttatcg
     1861 gtaaagacgt cgagctggta aaattcgtat tttctcataa ccggaattgc tgaattatga
     1921 acgatgaaag cggaattgta aatttccgtt ttgcccggga aaaccgttta ttttcacttt
     1981 tcttcaattt ttaccgtatt atcttgacat tcaatcactt aagagtgtaa atttttgtat
     2041 taatctgttc taatgagcgg cttatgaagt gatgcaaaga gcaaccctaa acactctttt
     2101 ctataggcat ttccgtaaaa ttccagccgg cgaattttca acttctaaag ctgtttgtag
     2161 gcgaaagcag tcccgcaagg tttcgtcgtg tgaaatatta cagattaaac gcgggcttgc
     2221 gcgcattatt tattagtagc tactttttga agggaattta acagaatttt tcatgacaaa
     2281 cgacgtaaca aaacaaactg aagatacgac ggcggaaaat aatcagccga cgaattcagt
     2341 tacggatgaa acaaaaaact ccgccggaac aatggcgggc gccacaactg atgaggaaca
     2401 aagctccaat gaaatggact ttggcgcgat tttaga
//
LOCUS       DASWNE010000944         1896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_102562, whole genome shotgun sequence.
ACCESSION   DASWNE010000944 DASWNE010000000
VERSION     DASWNE010000944.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102562"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..1385
                     /locus_tag="VGB68_18415"
     CDS             24..1385
                     /locus_tag="VGB68_18415"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEY0051276.1"
                     /translation="MYRLVRNQALRFVFLICCFVAASLDASAQQTFNKDEFRARRAKL
                     VERIGDGVAVIFGGESHTHAVKFRQSPDFYYLTGIEEPGSVLVVNGARKASYVFVPKR
                     TPGKIAAEGPGVRDMEKPTDFYGINVLPVENFYSISGFALSPASKLYLQLTPPDELQL
                     NRQEAFSHNAGMLENAVFRSEASITRLVGEMRKWKPEMQPADVSPLIDELRWVKSPYE
                     IERMRKAGQIGAESVREAMKGTKPGMFEYEIEAAARFINVKRGARGDAFIPIVASGPN
                     TIIYHYTKNNRQMQAGEVVYIDYGADYDYYTSDITRTWAVSGRFTAEQEKWYRCILEA
                     RNTIIAAMKPGARVRDLQKAAEPVYQKYGFLQDYINGGRYVGHFVGISVHDPGDETKP
                     FVPGVVFNVEPVIESSQQKIHMRLEDTVLITAAGAENLTAGVPAELEEVYALVSQPAF
                     GSK"
     gene            1620..>1896
                     /locus_tag="VGB68_18420"
     CDS             1620..>1896
                     /locus_tag="VGB68_18420"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051277.1"
                     /translation="MFDKLIGNGHVKEILQRMIAKRRVPNSLLFVGADGIGKREFALE
                     TAKAFACQNRRETGEACDACGACRRAEKFNFPNSRDKDDNEKIFFSEH"
BASE COUNT          469 a          476 c          519 g          432 t
ORIGIN      
        1 aaaataaaaa ggaataattt tttatgtatc gcttagtcag aaatcaggct ttaagatttg
       61 tgtttttaat ttgctgcttt gtcgccgctt cgctcgatgc gtcggctcag cagacgttta
      121 ataaagacga gtttcgcgcg cgccgagcga aattggtcga gcgcatcggc gacggcgtcg
      181 ccgttatttt cggcggcgaa tcgcacacgc acgcggtcaa gtttcgccag tcgcccgatt
      241 tttattatct gaccggaatc gaagaacccg gctcggtgct ggtcgtcaac ggcgcgcgca
      301 aagcgagcta cgttttcgtc ccgaaacgca cgccgggcaa aatcgcggcg gaaggtccgg
      361 gcgttcgcga tatggaaaag ccgacggact tttacggcat aaatgtttta ccggtcgaaa
      421 atttttactc gatttccggt ttcgcgctta gccccgcgag caaattgtat ctgcaattga
      481 cgccgccgga cgagcttcag ctgaatcgtc aggaagcctt cagtcacaac gccggtatgc
      541 tggaaaatgc ggttttccgc agcgaagcgt cgatcacaag gctcgtcggc gagatgcgga
      601 aatggaagcc ggagatgcag ccggcagacg tcagcccgct gattgacgag ctgcgctggg
      661 tgaaatcgcc atacgaaatc gagcggatgc gaaaagccgg acaaatcggc gcggaaagcg
      721 tgcgcgaggc gatgaaggga acaaagcccg gcatgttcga atacgaaatc gaagcggcgg
      781 cgcgttttat caacgtcaag cgcggcgctc gcggcgacgc ctttataccg attgtggctt
      841 cgggtccgaa cacgattatt tatcattaca cgaaaaataa tcgacagatg caggcgggcg
      901 aagtcgtcta tatagattac ggcgcggatt acgattacta cacttccgat attacgcgga
      961 cgtgggctgt gtcgggacgc tttacggcgg aacaggagaa atggtatcgc tgcattctgg
     1021 aagcgagaaa cacgattatc gccgcgatga aaccgggcgc gagagtcaga gatttacaaa
     1081 aggcggcgga gccggtttat caaaaatacg gctttttgca ggattatatc aacggcggac
     1141 gctacgtcgg gcattttgtc ggaatctccg tccacgaccc cggcgacgag accaagccgt
     1201 ttgtaccggg cgttgttttc aacgtcgagc cggtcatcga atcttcccaa caaaaaattc
     1261 atatgcgtct ggaagacacg gttttgatta ccgccgccgg cgcggaaaat ctgacggccg
     1321 gcgttccggc tgagctcgaa gaagtatacg cgctcgtcag tcagccggcg ttcggctcaa
     1381 aataatcggg aatttttacc gccgcagggc acagagattt tttaaaaaga aattaaccgc
     1441 ggataacagg ctgcaaagag ataattaaaa tcattcttct tatcttcaga ccattaccgt
     1501 tattcccggt taatttcttt ttgcgtctac gcggtatttt tgcggttcat ttttctcacc
     1561 ccgcacactt catcaattcg gatttgcgtt ttcaaaaggc tgtagccgaa aatctatcta
     1621 tgttcgacaa actcatcggc aacggtcacg tcaaggaaat tttgcagcgg atgatcgcaa
     1681 agcgccgcgt tccgaattcg cttcttttcg tcggcgcgga cggaatcggc aaacgcgagt
     1741 ttgcgctgga gacggcaaag gctttcgcct gccagaaccg gcgtgaaacc ggcgaagcct
     1801 gcgacgcgtg cggcgcttgc cgacgcgccg aaaaattcaa ctttcctaat tccagagaca
     1861 aggacgacaa cgaaaagatt tttttcagcg agcacc
//
LOCUS       DASWNE010000945         3236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_315559, whole genome shotgun sequence.
ACCESSION   DASWNE010000945 DASWNE010000000
VERSION     DASWNE010000945.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315559"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..573)
                     /locus_tag="VGB68_18425"
     CDS             complement(187..573)
                     /locus_tag="VGB68_18425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051278.1"
                     /translation="MNRKFLYAILSLILVSIAIGGFSAFVRSVEAQNFDTCITDDGSG
                     ASLRFNSDNGDYIFCAGGKTFTGTGSVTKMGKVITLQHNAADRRLAASVNTGTRTGSG
                     MIQSPVGKTVGSISDRNTADSSCRCR"
     gene            complement(849..2018)
                     /locus_tag="VGB68_18430"
     CDS             complement(849..2018)
                     /locus_tag="VGB68_18430"
                     /inference="COORDINATES: protein motif:HMM:NF021466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008195 - phosphatidate phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase domain-containing protein"
                     /protein_id="HEY0051279.1"
                     /translation="MSDWKAKIESVIEELDNFSDKVKTRLRRIKGFDESLIIVPFLGY
                     GNADKFLLSGRVLADKGEVISSEMDGKRRNLVNLYKRFATDEVAGARVRAVFQDIERE
                     VMTDGEGYFNLELNLSQPLQSDSPFQEIDLELLEPLKEDGRTIRAVGRVLVPPPDAAF
                     GIISDLDDTIITTNVTNRLKMFLTVALLNEYTRMPFKGVSAFYRALQKGASGAENNPI
                     FYVSSSPWNLYQFLTEFLKLHNIPLGTLFLKDFGNHTIFNSGAHSAHKINSIESILNT
                     YPHLPFILIGDSGEKDPEIYREIVKKYPRRIRTIYIRSIDTKPGRLAAIDRLIAEIAD
                     TGCQLVLAPDTEFAAVHAAAERLIATGELPNIREEKKEDENAPKAREIVESNPEI"
     gene            2224..>3236
                     /locus_tag="VGB68_18435"
     CDS             2224..>3236
                     /locus_tag="VGB68_18435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-60 repeat domain-containing protein"
                     /protein_id="HEY0051280.1"
                     /translation="MKKASRVVFCVLVFFVFGFVFSESSVTAAPGDLDQSFGVQGRRI
                     VLIPNSQPNFLIWNTAQDIVVQPDGKILVAGGANDTNTGFSYTVVRFNTDGSLDSGFA
                     NGGVFRFEFGSGDDQAQGIALQPDGKIVVAGEANVNPFAADTAFGVIRLNPNGTFDTT
                     FGTNGVVTTNFFSSLDEATEVAIQPDGKIIATGFVTQGGDNNGLSYDFAAVRYNPNGS
                     LDTTFGGGDGIAFIDFNGRGDIAQTSVLQPDGKIVLAGWVSIVAGADYDYGLARLNAD
                     GSPDTGFDGDGKVVTTFGEADWNELARGMALAPDGKIVVTGDSYNPPPVIGQSGNWDI
                     TVAR"
BASE COUNT          753 a          814 c          824 g          845 t
ORIGIN      
        1 accgtcgatt tgatgcgaaa ccttgccgga gattttgacg gagacgggaa agcggatttg
       61 gcggcgttca gaccaccgag cggaatttgc tatctgctac gctcggcgca ggtttttacc
      121 ggcgtccggt tcgggacaaa aggcgatttg cccatttcga acgcttacat cagataaaat
      181 ttgaatttag cgacaccggc aactgctgtc cgccgtgttg cggtcgctga ttgagccgac
      241 ggttttgccg accggcgatt gtatcatacc ggagccggtt ctggttcccg tgttgacgct
      301 ggcagccagt cggcggtcgg cggcgttgtg ctggagcgtg atgaccttgc ccattttcgt
      361 caccgagccg gttccggtaa atgtttttcc gcccgcgcaa aatatataat cgccgttgtc
      421 ggagttaaaa cgcagcgacg cgccgctgcc gtcatcggta atacaggtat cgaaattctg
      481 cgcctcgacg ctgcgcacaa aagcgctgaa gccgccgata gcgattgaaa ccagaattaa
      541 acttaaaatt gcgtataaga atttccgatt catattttac tccttttgtg tgccgtaaat
      601 ttacataata ttccgcttaa aagcaagatt tttcggaaca cttaaattag atgcataatt
      661 taaatatatt tgcgttaaag aatttccata ctgcatgaac aggatgaatt cagaggcttt
      721 cagaaaagaa cttttcaggg ttaggcgggc ttcgcggctt gataaacaag gataagaagg
      781 atattttttt atccctgctt tcccggctat tctcgtttaa ttcttcttcg tgctgaattt
      841 tccggcaatt aaatttcagg attgctttcg acaatttccc gtgctttcgg cgcgttttcg
      901 tcttctttct tttcctcgcg gatatttggc agttcgccgg tcgcaatcaa tctttcggcg
      961 gcggcgtgaa cggcggcgaa ttccgtgtcc ggcgcgagca cgagctggca gccggtgtcg
     1021 gcgatttccg caatcaatct gtcaatcgcg gcgagacgcc ccggttttgt gtcgatgctg
     1081 cggatgtaaa tcgtgcggat tcgacgcgga tattttttga cgatttcgcg atagatttcc
     1141 gggtcttttt cgccgctgtc gccgattaaa ataaacggca aatgcgggta tgtattcaga
     1201 atcgactcga tgctgtttat tttgtgagcc gaatgcgcgc cggaattaaa aatcgtgtga
     1261 ttgccgaaat ctttcaaaaa cagagttccg agcggaatat tgtgcaattt taaaaactcg
     1321 gtcagaaact ggtataaatt ccacgggctg ctcgaaacgt aaaaaatcgg gttgttttcc
     1381 gcgccgctcg cgcctttttg cagcgcgcga tagaaagccg atactccttt aaaaggcatc
     1441 cgcgtgtatt cgtttaacag cgcgacggtg agaaacattt tcagacgatt ggtgacattc
     1501 gtcgtgataa tcgtgtcgtc gaggtcgctg ataatcccga aagcggcgtc cggcggcgga
     1561 accaaaacgc gaccgacggc tcgaatcgtc cgcccgtctt cttttaacgg ctccagcaat
     1621 tccaaatcga tttcctgaaa cggcgaatcg ctttgcaaag gctggctcaa atttaattcc
     1681 agattaaaat aaccttcgcc gtcggtcata acttctcttt cgatgtcttg aaaaacggcg
     1741 cgaacgcgcg ctccggcaac ctcgtcggtc gcgaaacgct tgtataaatt gaccagattg
     1801 cgacgtttgc cgtccatctc gctggaaatc acctcgcctt tatccgccag cacgcgcccg
     1861 ctgagcaaaa atttatcggc gttgccgtaa ccgagaaacg gaacgattat caaagactcg
     1921 tcaaaacctt tgattctgcg taaacgcgtc ttaaccttat cggaaaaatt gtccagctct
     1981 tctattacgc tttcgatttt cgctttccaa tcactcatta ttttaaatta tcacttaaaa
     2041 agcccgcttc aaaaagcgtc gaatgccgcg ctttttctgc taaattcaac gaacaaataa
     2101 aatttccgcg tcaaacaaaa attttcttgt attttttcat acggtttacg acactgtgta
     2161 aatacgattt tacaaaaatc aatcgtcgtc cgcgtccacc gaacgaacat cttgggaggg
     2221 tttatgaaaa aggcttcgcg cgtagttttt tgtgtactgg ttttctttgt tttcggtttc
     2281 gtgtttagcg agtcgagcgt cacggcagcg ccgggcgacc tcgaccaatc gtttggcgtg
     2341 cagggcaggc gaatagttct gattccgaat tcccagccga attttttgat ttggaatacg
     2401 gcgcaggaca tcgtcgtcca gccggacgga aaaattctgg tcgccggcgg cgcaaacgat
     2461 acgaacaccg gtttcagcta caccgtggtt cgatttaaca cggacggaag cctcgattcc
     2521 ggttttgcca acggcggcgt ttttcgtttt gaattcggca gcggcgacga tcaggcgcag
     2581 ggaattgcgc ttcagccgga cggaaaaatc gtcgtcgcgg gcgaagccaa cgttaatccg
     2641 ttcgctgccg acacggcttt cggcgttatc cggcttaatc cgaacggcac gttcgatacc
     2701 actttcggga cgaacggcgt cgttaccaca aattttttct caagcctcga cgaagcgacg
     2761 gaagtcgcca ttcaacccga cggaaaaatc atcgccaccg gatttgtcac gcagggcggc
     2821 gacaacaacg gcttgtcata tgattttgcc gctgtccgct ataacccgaa cggcagcctg
     2881 gatacgacct tcggcggcgg agacggaatc gcttttatag actttaacgg cagaggcgac
     2941 atcgctcaga cgtccgtgct gcagccggac ggaaaaatcg tgctggcagg ctgggtttcc
     3001 atcgtcgccg gcgcggatta tgattacggg ctggcgcggc tgaacgcgga cggctcgccg
     3061 gatacaggct ttgacggcga cggaaaagtc gtcaccactt tcggcgaagc cgattggaat
     3121 gagcttgcgc gcggtatggc gctcgcgccc gacggcaaaa tcgtcgttac cggcgactca
     3181 tacaatcctc cgccggttat cggtcaaagc ggcaattggg atataacggt tgctcg
//
LOCUS       DASWNE010000946         4591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_667904, whole genome shotgun sequence.
ACCESSION   DASWNE010000946 DASWNE010000000
VERSION     DASWNE010000946.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667904"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VGB68_18440"
     CDS             <1..209
                     /locus_tag="VGB68_18440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051281.1"
                     /translation="FKEKIVRNNDRYFQIDENGRPVSDPFCSSCWDSEQKVIYLVSPQ
                     EDNENYHKCPKCKNEYGSPKIYFG"
     gene            complement(213..3305)
                     /locus_tag="VGB68_18445"
     CDS             complement(213..3305)
                     /locus_tag="VGB68_18445"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0051282.1"
                     /translation="MENEPKSLTGLARKLRNLPTDQKRAALEMSASLAGVSLRVSRAF
                     VEATPDAAKILDAETLRAWAEMGRKLAMANVDAGVKFFGDGIENFREVPAKARPFVFQ
                     ICMRQLVLSSSIAVETFNEIPRLAIEVDDDELFTDILKVANEVAQRSAKHSSDFLKAT
                     PKLAESLKFFDAEKRKVSKAIVSLATNFASRTGGMTADLWQILPDALQKLSAERAVRL
                     AKKAAEFLEYGGSVTLHFISAGGESLRRVEAVFGDWCEVLQKVAKHGNAVLVAFLRSS
                     PGFFAQISSVRNEAEIVSISRRVLQLVREIAETDAESALAAFRSSASALRKVSLVQFE
                     DWVLSGLQNNSANARARRSYFALETRQSHDFLHEGQEGLPLEKVQTILRIYVEGLTGK
                     EVEVAPLSAMPQESRFGDGKTIYLPATVAEFKDDELDFRLYKVLSAHAAGQIEFGTYD
                     RDSNGLKAAFAELNDLYEATAEQRDAFSLAGYIEDVQKGEKSLSDDEIRAELKKRARK
                     LPKNSDYRAVLQIFPEPSLAKKIFGTLENARIDRRLRKTYRGLVRDLDLMQAHLREKR
                     PFIFDLPVHQVPFELLFQITLCGGATDDSRKFYAQIVSEIESVIDSYLHDKTATVADT
                     LMATSRVYTLFQNISTESQAQESPENEDEKGEYAYEDKNSGEAVTENQIKREERPQKA
                     QDVRDLFSAWNNLEEDDGDPDDLQGAETWTHNEIPEQPLEAGDAAFSYDEWDRDLADY
                     RIGWSRVIEKKVRQGDRNFVELTRSRYRGVISSVRHQFQLMKPENLQKINREIDGEDY
                     DLNALVEYVVDRRADGQQSERIYTKRLRKQRDVAVSILLDQSSSTARTITRNPLQPYT
                     HPGRRIIEIEKEGLVLMSEALEAVGDVYSINGFTSEGRRNVKFYVVKDFDEKYSDEIE
                     RRIGGITFQNNTRLGAAIRHAAAKLLRQDSRTKLMIILTDGRPYDHDYGDARYAREDV
                     REALTEAKLNGITPFCITIDRESEAELRDLYGDVGYTIIDDVLSLPERMPNIYRRLTS
                     "
     gene            complement(3535..>4591)
                     /locus_tag="VGB68_18450"
     CDS             complement(3535..>4591)
                     /locus_tag="VGB68_18450"
                     /inference="COORDINATES: protein motif:HMM:NF039204.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5715 family protein"
                     /protein_id="HEY0051283.1"
                     /translation="TALALLLANSFGGGAGGKGDGNPTPAIIRSGLLIPVRVVDPLGG
                     EDPEELDDLIALFEVEEKELKVEDLGEVKSIAVDDSTKPSELNVPITFWQKQRDLVFA
                     PRTGVTGISPPGMEVPPELRGDGVVKQKAKLAEMMRGGYQQPMDFAELAQKRLSKELV
                     ELPMATDSFLLDVGGSATESDFTSFSFQSRDTPIAPNTPKYQTLLELANNFDGQKYDL
                     NNGRDRRQMRIRLLRMFHPRARPILKELADAYFQEFKRPLRVTSLTRSMDYQISLNRT
                     NANSFIVRGPGSLPPHTSGCAFDLGRAYMTVKEQNFVMKKLAEMENRGVLDALIEYNV
                     NACWHVFIYPDGKPPKM"
BASE COUNT         1019 a         1225 c         1189 g         1158 t
ORIGIN      
        1 agtttaaaga gaaaatagtt agaaataatg ataggtattt tcaaattgat gaaaacgggc
       61 gtccagtaag cgaccctttt tgctcaagtt gttgggattc tgaacaaaag gtcatttatt
      121 tggtttctcc tcaagaagat aatgaaaact atcataaatg tcctaagtgt aaaaatgagt
      181 atggaagtcc taaaatctac tttggttaaa ttctaactcg tcaaacgtcg ataaatattc
      241 ggcattctct ccggcaaaga taaaacatcg tcaataatcg tatagccgac atcgccgtat
      301 aaatctctca attcggcttc cgattcgcgg tcaatcgtga tgcagaaagg tgtgatgccg
      361 tttagtttcg cttcggtcag ggcttcgcgg acgtcttcgc gggcgtagcg ggcatcgccg
      421 taatcgtggt cgtaaggtct gccgtcggtc aggattatca ttaatttcgt gcgcgaatcc
      481 tggcgcagaa gctttgccgc cgcgtgacga atcgccgcgc ctaaacgcgt attgttttga
      541 aacgtgatgc cgccgatgcg ccgttcgatt tcgtcggaat atttttcgtc gaaatccttg
      601 acgacataga atttcacgtt gcgccgacct tccgatgtaa agccgtttat cgaataaacg
      661 tcgccgacgg cttcgagcgc ctcgctcatt aaaaccaagc cttctttctc gatttcgata
      721 atgcgccgtc ccgggtgcgt gtagggctgg agcggattgc gcgtaatcgt gcgcgccgtc
      781 gaggaagact ggtcgagcag aatcgaaacg gcaacgtcgc gctgttttct gaggcgtttc
      841 gtataaattc tttccgattg ctgaccgtcc gccctgcggt cgacgacgta ttcgaccagc
      901 gcgtttaaat cgtaatcttc gccgtcgatt tcgcggttga ttttttggag gttttccggt
      961 ttcatcaact ggaattgatg ccttacggaa gaaatgacgc cgcgatagcg cgagcgcgtc
     1021 agctcgacaa aattgcggtc gccctgacgg acttttttct cgatgacgcg gctccagccg
     1081 atgcggtaat ccgccaaatc cctgtcccat tcgtcgtacg aaaaagccgc gtcgcccgct
     1141 tccagaggct gttcgggaat ctcgttgtgc gtccacgttt ccgcgccttg caaatcgtcc
     1201 gggtcgccgt cgtcttcctc cagattgttc cacgcgctga ataaatcgcg tacgtcctgc
     1261 gccttttgcg gtcgttcttc gcgcttgatt tgattttcgg tgacggcttc gccggaattt
     1321 ttatcctcgt aggcgtattc gcctttctcg tcctcgtttt ccggcgattc ctgcgcctgg
     1381 ctctcggtcg aaatattctg gaaaagcgtg taaacgcggc tcgtcgccat caaggtatcg
     1441 gcaacggtcg cggttttatc gtggagataa gaatcgatga cgctttcgat ttccgagacg
     1501 atttgcgcgt aaaatttacg agaatcgtcg gtcgcgccgc cgcagagcgt aatttgaaac
     1561 aaaagctcga acggcacctg atgaacgggc aaatcgaaaa tgaacgggcg tttttcgcgc
     1621 agatgagcct gcatcaaatc caaatctctg accaagccgc gataagtttt tcttaaacgt
     1681 ctgtcgattc gcgcgttttc cagcgttccg aaaatctttt tcgccaggct cggttcggga
     1741 aaaatctgca aaacggctcg ataatcggaa ttttttggaa gttttcgagc gcgttttttc
     1801 aattccgcac ggatttcatc gtcgctcaga gacttttcgc ctttctgcac gtcttcgatg
     1861 taaccggcga gcgaaaaagc gtcccgctgc tcggcggtcg cttcgtataa atcattcagc
     1921 tcggcgaaag cggctttcaa tccgttcgag tcgcggtcgt aagttccgaa ttcgatttgc
     1981 ccggcggcgt gcgccgaaag cactttgtaa aggcgaaaat ccagctcgtc gtctttaaat
     2041 tcggcgaccg tggcgggcag gtaaatcgtt ttgccgtcgc cgaaacgcga ttcctgcggc
     2101 atcgccgaaa gcggcgcgac ttcgacttct ttgcccgtca atccttcgac gtaaatccgc
     2161 aaaatggtct ggactttttc gagcggcaag ccttcctgac cttcgtgcag aaaatcgtgg
     2221 ctctgacgcg tttcgagcgc gaaataactg cggcgcgcgc gcgcgttggc ggaattattc
     2281 tgcaagcccg acaaaaccca gtcttcaaat tgaacgagcg aaactttccg cagcgccgac
     2341 gcgctggagc gaaaagccgc taaagcgctt tcggcgtcgg tttcggcgat ttctctgacc
     2401 agctgcaaaa cgcggcgcga aatcgaaacg atttccgctt cgtttctgac gctcgaaatc
     2461 tgcgcgaaaa agccgggcga agaacgcagg aaggcgacta aaacggcatt tccgtgtttc
     2521 gcgacttttt gcaaaacttc gcaccaatcg ccgaaaacgg cttcgacgcg ccgcaaggat
     2581 tcgccgcccg ccgagatgaa atgaagcgtc acgctgccgc cgtattcgag aaactccgcc
     2641 gcttttttcg ccagcctgac ggctcgctcg gcggaaagct tttgcagcgc gtcgggcaaa
     2701 atctgccaca aatcggcggt cattccgccc gtgcgcgaag cgaaatttgt cgccagcgaa
     2761 acgatcgctt tcgaaacttt gcgcttttcc gcatcgaaaa attttaaaga ttccgccagc
     2821 ttcggcgtcg ctttcagaaa atccgagctg tgtttggcgc tgcgctgcgc gacttcgttg
     2881 gcgactttta aaatatcggt aaacaattcg tcgtcgtcga cttctattgc cagacgcgga
     2941 atttcgttaa aagtttcgac ggcaatcgag ctggacaaga cgagctggcg catacaaatc
     3001 tgaaaaacaa acgggcgcgc tttcgccgga acttcgcgaa aattttctat gccatcgccg
     3061 aaaaatttaa ctccggcgtc gacattcgcc attgctaatt tccgtcccat ttccgcccag
     3121 gcgcgtagag tttcggcgtc cagaatcttg gcggcgtccg gcgtggcttc gacaaaagcg
     3181 cggctgacgc gcagggaaac tccggcgagc gacgcgctca tttccagcgc cgcgcgcttc
     3241 tggtcggtcg gcagatttcg caatttgcgg gctaatccgg tgaggctttt cggctcgttt
     3301 tccatatttc aagtttagca aaattagccg cagagaacac agatagagaa cggagaaaga
     3361 aaatttaacc gctgatgaac gtagatttga acaagataaa tcgagcaagc agtataaaaa
     3421 taaatctgcg ctaatcattg tcagtccgtt gtttcaaaat ttttttatcc gcgtttatcg
     3481 gcgaaaatct gcggtttttc tttttcccgc gcgctgtttg cggctaaaaa aaaattacat
     3541 tttcggcggc ttgccgtccg gataaataaa gacgtgccag caggcgttga cgttatattc
     3601 gataagcgcg tccagaacgc cgcgattttc catttcggcg agttttttca tcacgaaatt
     3661 ctgctctttg accgtcatat atgctcgtcc gaggtcaaag gcacagcccg aagtatgcgg
     3721 cggcagactg cccggaccgc gcacgataaa cgaattggcg ttcgttctgt tcaggctgat
     3781 ttgataatcc atcgagcgtg tcaggctggt cacgcgcagc gggcgtttga attcctgaaa
     3841 ataagcgtcg gcgagctctt ttaagatggg tcgggcgcgc gggtgaaaca ttctcagcag
     3901 ccggattctc atctgccggc ggtcgcgccc gttgttcaaa tcgtatttct gaccgtcgaa
     3961 attattggcg agctcaagca gagtttgata ttttggcgtg ttcggcgcaa tcggcgtatc
     4021 gcggctctga aaactgaacg acgtaaaatc gctctcggtc gcgctgccgc cgacgtcgag
     4081 cagaaagcta tcggtcgcca tcggcagttc gaccaattcc ttgcttaaac gcttttgcgc
     4141 cagttcggca aaatccatcg gctgctggta accgccgcgc atcatttcgg cgagcttggc
     4201 tttttgtttg acgacgccgt cgccgcgcag ctccggcgga acttccattc ccggcggcga
     4261 gatgccggtc acgcccgtgc gcggcgcgaa aaccaaatcg cgctgctttt gccagaaggt
     4321 aatcggcaca ttcaattccg aaggcttcgt cgaatcgtca accgcaatcg atttaacttc
     4381 gccgaggtct tcgactttca gctctttttc ttcgacttca aataaagcta tcaaatcgtc
     4441 caactcttcc gggtcttcgc cgccgagcgg gtcgaccacg cgcacgggaa tcagcaagcc
     4501 ggagcgaata atcgcgggcg tcggattccc gtcgcccttt ccgcccgcgc cgccgccgaa
     4561 cgagtttgcc agcaaaagcg ccagcgcggt g
//
LOCUS       DASWNE010000947         2683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_66867, whole genome shotgun sequence.
ACCESSION   DASWNE010000947 DASWNE010000000
VERSION     DASWNE010000947.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66867"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VGB68_18455"
     CDS             <1..188
                     /locus_tag="VGB68_18455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051284.1"
                     /translation="NSDRADFVFEIAIFLSGIAIPKASIADFGFRIAESCFGLRRFRF
                     SYRDTLNEPPRASSPVS"
     gene            complement(247..1251)
                     /locus_tag="VGB68_18460"
     CDS             complement(247..1251)
                     /locus_tag="VGB68_18460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY0051285.1"
                     /translation="MVRRSASNVLKRITIGAHHEHHHEHDRRGAGKQTRLKIALGLTF
                     AYMLAEAVGGWLVNSLALLADAGHMLTDVAALSLTLAAIWFASRPATAKKTFGYYRLE
                     ILAAFVNGVALVLISLFVIYEAYERWAAPPEIKGGSLTVIAAGGLLVNIVCAYILHGD
                     HQHDLNMRGAWLHVIGDALGSVTAIIAGVLIIAFGWLWADAACSILISLIIVFSAWNL
                     IRESVNVLLEGTPAHINLAAVEDEILDTEGVNGIHDLHIWTITSGLEALSAHVRHDEG
                     IVQTELLKKLRQRLHDKFGIDHLTIQMETPDFEDETVHFCDTGAKCFESEVRIKTVNN
                     "
     gene            complement(1300..2538)
                     /locus_tag="VGB68_18465"
     CDS             complement(1300..2538)
                     /locus_tag="VGB68_18465"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEY0051286.1"
                     /translation="MEPKYRKFYVDWWNIKKSHVYGVIGTVVFLGLLIGAGWWVVKSG
                     WLFADPDNMEIPKDAARLESFEGDVRITRAATRQTEKVTTKTYVLAGDTIQTQADGRA
                     QIRMIDGAVLSIRPNSTVVIRNSTSIFGGTDVRVSLGDGQINVKTENQTEASQNIVEI
                     KESENRLFSQTDASFNINQKTSGGEIRISRGGVETNIGGEKTVIKDNEFATVNNGRLS
                     PKEKLLDAPKLIAPPALEQILTSSSGRADVTFRWQKTEGAAGSYHLQVSTSPFFVADG
                     MVKEADTLVAPSLLLANAAPGTYYWRIRATAASGQASEWSEPWRFTVLKREEGDVINA
                     TDWLVENLGGRVYRINGKTQPGATVRILGRETFATGDGGFVLQVVATSPEVTVEISDE
                     HGNRRRFVLSLDSAKVVRQY"
BASE COUNT          559 a          787 c          695 g          642 t
ORIGIN      
        1 aaaattctga ccgtgcagat ttcgtctttg aaatcgcgat ttttctctct ggtatcgcga
       61 ttccaaaggc aagtatcgca gatttcggct ttcgtattgc agaatcttgc ttcggattgc
      121 gcagattccg cttttcgtat cgcgatactt taaacgagcc gccgcgcgct tcaagtccgg
      181 tttcgtgata cttaagggca aatgtcgcga ttcggcagcc ggattcttta ctgccaaagg
      241 cttattttag ttatttacgg tctttatcct gacttccgac tcgaaacatt tcgcgcccgt
      301 atcgcagaaa tgaacggttt cgtcttcgaa atcgggcgtc tccatctgaa tcgtcaaatg
      361 gtcgatgccg aatttatcgt gcaggcgctg gcgcagtttt tttaaaagct cggtctgaac
      421 gattccttcg tcgtggcgaa cgtgtgccga aagcgcttcg agtcccgagg taatcgtcca
      481 gatgtgcaaa tcgtgaatgc cgttgacgcc ttcggtgtcg agaatctcgt cttcgaccgc
      541 cgccagattg atgtgcgcgg gcgtgccttc caaaagcacg ttgaccgatt cgcgaatcag
      601 attccacgcg ctgaaaacga taatcaaact tattaaaata ctgcacgccg cgtccgccca
      661 taaccagccg aaagcgataa tcaaaacgcc ggcgataatc gccgtcaccg agccgagagc
      721 atcgccgatg acgtgcagcc acgcgccgcg catattcaaa tcgtgctggt ggtcgccgtg
      781 taaaatatat gcgcagacga tattgaccag cagaccgccg gcggcgatga cggtcagcga
      841 gccgcccttg atttccggcg gcgcagccca gcgctcatag gcttcgtata taacaaacag
      901 cgaaatcagc acgagcgcca cgccgttgac gaaagcggcg aggatttcca gacgataata
      961 accgaaagtt tttttcgccg tcgccggacg cgaagcaaac caaatcgccg ccagcgtcag
     1021 gctcagcgcg gcgacgtcgg tcagcatatg cccggcgtcg gcgagcagcg ccagcgagtt
     1081 gaccagccag ccgccgaccg cttccgccag catatacgca aacgtcaaac cgagcgctat
     1141 cttcagccgc gtctgttttc ccgcgccgcg ccggtcgtgt tcgtgatgat gctcgtgatg
     1201 tgctccgata gttatcctct ttaaaacgtt ggatgcggaa cgacgaacga ttaattcgtt
     1261 ttttctttca tcgtttcgcg ttcatctttc atcatttcat taatattgcc tgacaacttt
     1321 tgccgaatcg agcgagagca cgaagcggcg gcggttgccg tgttcgtcgc tgatttcgac
     1381 cgttacttcc gggctggtcg caacgacctg caaaacaaag ccgccgtcgc cggtggcaaa
     1441 cgtttcgcgt cccagaatcc gcacggtcgc gccgggctga gtttttccgt tgatgcggta
     1501 aacgcgcccg cccaaatttt ccaccagcca atcggtcgcg ttgatgacat cgccttcctc
     1561 gcgtttcaga accgtgaaac gccacggctc gctccattcg ctcgcctgtc cggaagcggc
     1621 ggtcgcgcgg attcgccagt aataagttcc cggcgcggcg tttgccagca gcaggctcgg
     1681 cgcaaccagc gtgtccgctt ccttgaccat tccgtcggcg acgaaaaacg gcgaggtcga
     1741 aacctgcaag tgatacgacc cggcagcgcc ttcggttttc tgccagcgaa acgtcacgtc
     1801 cgccctgccg ctcgaagacg ttagaatttg ttcgagcgcg ggcggcgcaa tcagcttcgg
     1861 cgcgtccaaa agcttttcct tcggcgacag cctgccgtta ttgaccgtcg cgaattcatt
     1921 atctttgatg acggttttct cgccgccgat gttcgtctcg acgccgccgc gactaattcg
     1981 gatttcgccg ccgctggttt tctggttgat gttaaagctg gcgtcggttt gcgaaaacag
     2041 tcggttttcc gattccttga tttcgacgat gttctgcgag gcttccgtct ggttttcggt
     2101 tttgacgttg atttgcccgt cgcccagaga aacgcgcacg tccgtgccgc cgaaaatcga
     2161 agtgctgttg cgaatgacga ccgtcgaatt cgggcgaatg gaaagcaccg cgccgtcaat
     2221 catccgaatc tgcgctctgc cgtcagcctg cgtctgaatc gtatcgcccg ccagaacgta
     2281 agtctttgtc gtcacttttt cggtctggcg cgtcgcggcg cgcgtaattc gcacatcgcc
     2341 ttcaaaagat tccagccgcg ccgcgtcctt cggaatctcc atattgtccg ggtcggcaaa
     2401 cagccatccg cttttgacca cccaccagcc cgcgccgatt agaagcccga gaaacacgac
     2461 agtcccaatc acgccgtaaa cgtggctttt cttgatgttc caccaatcaa cgtaaaattt
     2521 gcgatacttc ggttccatct tgaaagtggc tgacccgttt taattttaga ccgattcgga
     2581 aattgtggga aataatctca gcccgccggc ggctcgtccg cgccgggcat ctcctttttc
     2641 cgtgccggaa aaaccagcga taaaattacc gaaagcacca gag
//
LOCUS       DASWNE010000948         4759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_342814, whole genome shotgun sequence.
ACCESSION   DASWNE010000948 DASWNE010000000
VERSION     DASWNE010000948.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342814"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="VGB68_18470"
     CDS             <1..983
                     /locus_tag="VGB68_18470"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY0051287.1"
                     /translation="IGLRSKKVILYERNRLLTDAVKQRGITFTLTPEIEKELVSTGAD
                     KELVEAIRQKSPVVKATPTPLPKALPTPVAAPVPIPVATPTPVPSPPAPDFAFYRKRA
                     DENVGKGEYALAVNDYSRAIELNPKEVSVYLNRGLAHYNQKNYDLAAADYDKAIELNP
                     KDSMLYFNRGNSFEKMGNLQKAVGDYRKAFELDAGNEAAKLSLQRLQTEESKVLPKSS
                     APEKTSVSEPSKAPQNVELGALIDRAVRLVKPVYPPNAQKMNIEGQVTVQVSLDEEGN
                     VISAKATAGPPLLRTSCEDAARKSKFKPALSGTQAVKATGFITYNFKAAN"
     gene            complement(1077..1961)
                     /gene="deoC"
                     /locus_tag="VGB68_18475"
     CDS             complement(1077..1961)
                     /gene="deoC"
                     /locus_tag="VGB68_18475"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEY0051288.1"
                     /translation="MANLTNGNYEKLVEQITDLVLSRLDGSSGDGYDGYNCPTFCSAD
                     VQRVVDAGASRIGIVLGETATAHDWASLIDHTLLKPEASEADIRKLCDEAAQFGFASV
                     CVNPSWVKRASEFLRGTSVPVCTVIGFPLGATLPDVKAYEVRRAIFNGAREVDMVINI
                     GALKSGDDCTVEDDIRAVSEAAHENGVLCKVIIETALLTDEEKVRACLASKNAGADFV
                     KTSTGFAKGGATVEDVSLMRRVVGAALGVKASGGVKGIEDARAMFEAGATRIGASVGV
                     KIAQEASGVKSNIVAGNY"
     gene            complement(1967..2743)
                     /locus_tag="VGB68_18480"
     CDS             complement(1967..2743)
                     /locus_tag="VGB68_18480"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEY0051289.1"
                     /translation="MAEDKETRDRVRALVKEVLANVPVEDDEPQETFPQRVVVNSLKE
                     EIGREFDRDESAKSLITEDDLRGLEPGARLRVSENARFTPLADDLIREKAINLIKKSE
                     RRGSAKVQSIALACDHGGFEFKEQLKEHLAQSGFRVHDFGTNSKDAVDYPDFAHAVAR
                     AVSDKTVDVGIIIDGAGIGSAMTANKVPGVRAAACYSVALAKNSREHNGANVLTLGSA
                     QNNFNEIREIVEAFLTSDLTEDRHKKRVGKIEAIERQYKR"
     gene            2891..3442
                     /gene="hpt"
                     /locus_tag="VGB68_18485"
     CDS             2891..3442
                     /gene="hpt"
                     /locus_tag="VGB68_18485"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEY0051290.1"
                     /translation="MTTEFTNPNLETLYSADQIKARVGELGEQISRDYAGRDLVLVGV
                     LKGSVVFLADLMRAIDLKLTIDFMAVSSYKDGTVSTGDVEILKDLTNPIRGRDVIVCE
                     DIVDTGLTLFRLLEILGSRGANSLKIATLLDKPEPRIKKDLKIDYCGFQIPNKFVVGY
                     GLDAAGRYRNLPFIGVVKNPDEI"
     gene            3475..4374
                     /locus_tag="VGB68_18490"
     CDS             3475..4374
                     /locus_tag="VGB68_18490"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0051291.1"
                     /translation="MFRKFPVVFLLLSFSTPAVFAHNKHENGQDVTVRTQKIGGSVSM
                     LQGRGGNVGASTGADGILIVDDDYAEVSPKIAEALKALGSEKPRFILNTHWHGDHTGG
                     NRYFGKDSLIVAHVNVRRRLSVEQTIFGEKVQPYPESALPVITYQQTMSIHFNGEEIR
                     LVHFPNGHTDGDSVIFFTKSNVVHMGDHYFKGSFPFVDLASGGSVQGLMKNIADLIRQ
                     LPADAKIIPGHGALANLDDLKTYHRMLVETTDVVQKGINSGKTLEGLKKDGLPEKYKS
                     WGAGFIKTDFWIETIHKSLSTKK"
     gene            4407..>4759
                     /locus_tag="VGB68_18495"
     CDS             4407..>4759
                     /locus_tag="VGB68_18495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051292.1"
                     /translation="MQKIFLIFAVFCAVFGFSARIAAQDKTVDKTHVKQLEAIYRELD
                     AATKKRELKTYEKYLDKTFTIEKNGTKISRAQILELMKQFLATFAEITESTSKIEKIR
                     VADGSYFLETSSVLRG"
BASE COUNT         1156 a         1302 c         1209 g         1092 t
ORIGIN      
        1 taatcggctt gcggtcaaag aaagtaattt tgtatgaaag aaacaggctt ttgaccgacg
       61 ccgtcaaaca gcgcggaatt acttttacgc tgacgccgga aattgaaaag gagctggtca
      121 gcaccggcgc ggacaaagaa ctggtcgaag cgattcgaca aaaaagcccg gtcgtcaaag
      181 caacgccgac gcctttgccg aaagctctgc cgacgccggt cgccgcgccc gttcccattc
      241 ccgtcgccac tccgactccg gttcccagcc cgcccgcgcc cgattttgcg ttttaccgga
      301 aacgcgcgga cgaaaatgtc ggcaaaggcg aatacgcgct cgccgttaat gattacagcc
      361 gcgcgataga attaaacccg aaagaagttt cggtgtatct gaatcgcggg ctggctcatt
      421 acaaccagaa aaattacgat ttggcggcgg ctgattacga caaggcaatc gagcttaacc
      481 cgaaagattc gatgctgtat ttcaatcgcg gaaattcttt cgagaaaatg ggaaaccttc
      541 agaaagcggt cggcgattac cggaaagcat ttgagctgga cgccggcaac gaagcggcga
      601 agctttcact gcaaaggctt caaaccgaag aatcgaaagt tttgccgaag tcgagcgcgc
      661 cggaaaaaac ttcggtttcg gagccgtcca aagcaccgca aaacgtcgag ctcggcgcct
      721 tgatcgaccg cgccgtccgg ctggtaaagc cggtctatcc gccgaacgcc cagaaaatga
      781 atatcgaagg acaagttacg gttcaggtct cgctcgacga agaaggaaat gtcatttccg
      841 ccaaagcgac cgccggtccg ccgctgctcc gcacgagctg cgaagacgcg gcgcgcaaat
      901 cgaaattcaa gcccgcgctt tcaggcactc aggcggtcaa agcgaccggc ttcattacct
      961 ataatttcaa ggcggcaaat tgaaatcctg agccgcgagt ctttcaaaaa accgaagccg
     1021 tcttaaaaac tttttaagac ggcttctttc gttcgcggtt tcattttcga cggcttttaa
     1081 taatttcccg cgacgatatt cgattttacg ccgctggctt cctgcgcgat tttcacgccg
     1141 accgaagcgc cgattctggt tgcgcccgct tcaaacatcg cgcgcgcgtc ttcgattccc
     1201 ttgacgccgc ccgacgcctt gacgccgagc gccgcgccga cgacccggcg catcaacgat
     1261 acgtcttcga ccgtcgcgcc gcctttggca aaaccggtcg aggttttgac aaaatccgcg
     1321 cccgcgtttt tcgacgccag acacgcccgc actttttctt cgtccgtcag cagcgccgtt
     1381 tcgataatga ctttgcacaa cacgccgttt tcgtgcgcgg cttccgagac ggctcgaatg
     1441 tcgtcttcga ccgtgcagtc gtcgccggat tttaaagcgc cgatgttgat gaccatatcg
     1501 acttcgcgcg cgccgttaaa aatcgcgcgc ctgacttcgt aagctttcac gtcgggcaaa
     1561 gtcgcgccga gcggaaaacc gatcacggtg caaaccggaa cgctcgtccc gcgcaaaaat
     1621 tcgctcgctc ttttgaccca gctcggattg acgcagacgg acgcaaagcc gaactgcgcg
     1681 gcttcgtcgc aaagttttct gatgtcggct tcgctcgctt cgggtttgag cagagtgtgg
     1741 tcaatcaggc tcgcccaatc gtgcgccgtc gccgtttcgc ccagcacaat gccgatgcgc
     1801 gaagcgcccg cgtcaaccac gcgctgcacg tcggcgctgc aaaacgtcgg gcagttatat
     1861 ccgtcgtagc cgtcgccgct gctgccgtcc agacgcgaca aaaccaaatc cgtaatctgc
     1921 tcgaccagtt tttcgtaatt tccgttggtt agattagcca ttttttttaa cgtttatatt
     1981 gtcgctcaat cgcttcgatt tttccaacgc gttttttatg gcggtcttcg gtcaaatccg
     2041 aagtcagaaa agcctcgacg atttccctga tttcattaaa attattttgc gcgctgccca
     2101 gagttagaac attcgcgccg ttatgctcgc gcgaattttt cgccagcgcg acgctgtagc
     2161 aagccgccgc gcggacgccg ggaactttgt ttgccgtcat cgccgagcca atcccggcgc
     2221 cgtcgatgat aattccgacg tcgaccgttt tgtcggaaac ggcacgggcg acggcgtgcg
     2281 cgaaatcggg ataatcgacc gcgtcttttg aattcgtgcc gaaatcgtgg acgcggaaac
     2341 cggattgggc gagatgttct ttgagctgtt ccttgaattc aaaaccgccg tggtcgcaag
     2401 ccagggcgat tgactggact ttcgccgagc cgcgtctttc tgattttttg atgagattaa
     2461 tcgccttttc gcggattaaa tcgtcggcga gcggcgtaaa tctggcgttt tcggaaacgc
     2521 gcaggcgcgc gcccggttcc agcccgcgca aatcgtcttc ggttatcagg cttttcgccg
     2581 attcgtcgcg gtcaaattcc ctgccgattt cttctttgag cgaattgacg acgacgcgct
     2641 gcggaaaagt ttcctgcggc tcgtcgtctt cgaccggaac atttgccagc acttctttaa
     2701 cgagcgcgcg cacgcggtca cgggtttctt tatcttcagc cataattttt tagtccggag
     2761 agcccggaga gcggcgagcc ggacggaaat ttcttttgac tttccagact cccggctctc
     2821 ccggctctcc ggacttgtaa cttatcactt gtttgaaata gtatttctaa aaccattttc
     2881 aatcaaactt atgacaaccg aatttaccaa tccgaatctc gaaactttat attcagccga
     2941 ccagatcaaa gcgcgcgtcg gcgagctggg cgagcagatt tcgcgcgatt atgccggcag
     3001 agacttggtt ttagtcggcg ttttaaaagg ctcggtcgtt tttctagccg atttgatgcg
     3061 cgccatcgac ttgaaactga cgatagattt tatggcggtt tccagctaca aggacgggac
     3121 ggtttcgacc ggcgacgtgg aaattttaaa ggatttgacc aacccgattc gcgggcgcga
     3181 cgtcatcgtc tgcgaagaca tcgtcgatac gggcttgacg ctttttcgcc tgttggaaat
     3241 tttaggctcg cgcggcgcga attctttaaa aatcgccacc ttgctcgata aaccggagcc
     3301 gcgcatcaaa aaagatttga aaatcgacta ttgcggcttt caaatcccaa ataaattcgt
     3361 cgtcggctac ggtttggacg ccgccggcag ataccgaaat ctgccgttta tcggcgtggt
     3421 gaaaaacccc gacgaaattt aattcaaaac gaaattaatt taatggagaa aagaatgttc
     3481 agaaaatttc cggtcgtatt tcttttactg tctttttcta cgcccgccgt ctttgcccat
     3541 aataaacacg aaaacgggca ggacgtaacg gtcagaacgc aaaaaatcgg cggcagcgtt
     3601 tcgatgctgc aaggtcgcgg cggaaacgtc ggcgcatcaa ccggcgcgga cggcattttg
     3661 attgtcgacg atgattacgc ggaggtttcg ccgaaaatcg ccgaagcttt aaaagcgctg
     3721 ggctcggaaa aaccgcgttt tattttgaac acgcactggc acggcgacca tacgggcggc
     3781 aacagatatt tcggcaaaga ctcgctcatc gtcgcgcacg ttaacgtccg ccggcggctt
     3841 tccgtcgaac agacgatttt cggcgaaaag gttcagccgt atccggaatc ggcgctgccc
     3901 gttattacct accagcaaac gatgtcgatt catttcaacg gcgaggaaat ccgcctcgtc
     3961 catttcccga acgggcacac cgacggcgat tccgttatct ttttcaccaa atcgaacgtc
     4021 gttcatatgg gcgaccatta tttcaaaggc agctttccgt ttgtcgattt ggcgagcggc
     4081 ggcagcgttc agggtttgat gaaaaatatt gccgacctta tcaggcagct tccggcagac
     4141 gcgaaaatca ttcccggtca cggcgcgctg gcaaatctcg acgatttgaa aacttatcat
     4201 cgaatgctgg tcgaaacgac cgacgtcgtg cagaaaggca tcaatagcgg caagactctg
     4261 gaaggcttga aaaaagacgg cttgcccgaa aaatataaat cgtggggcgc gggctttatc
     4321 aaaaccgatt tctggattga aacgattcac aaaagcctct cgacaaaaaa ataaattcga
     4381 agcgaaaaaa aaggaattca aacaaaatgc aaaaaatatt tcttatcttc gccgtcttct
     4441 gcgccgtctt cggatttagc gcgcgcatcg ccgcacagga caaaacggtt gacaagacgc
     4501 acgtcaaaca gctcgaagcg atttaccggg agctggacgc ggcgacgaaa aaacgcgagc
     4561 tgaaaactta cgaaaaatac ctcgacaaaa cttttacaat cgaaaaaaac ggcaccaaaa
     4621 tctcgcgcgc gcagattctc gaattgatga agcagtttct ggcgacattc gcagaaataa
     4681 ccgaatcaac ttcgaaaatc gaaaaaatca gggtcgccga cggcagctat tttctggaaa
     4741 cctcaagcgt cctccgagg
//
LOCUS       DASWNE010000949         3667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_31190, whole genome shotgun sequence.
ACCESSION   DASWNE010000949 DASWNE010000000
VERSION     DASWNE010000949.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31190"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VGB68_18500"
     CDS             complement(<1..615)
                     /locus_tag="VGB68_18500"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY0051293.1"
                     /translation="MSEQAGKLVGVEVSNLVLRAVCLNQAGEAVDLQEAPIDRRESVT
                     SQLIAFINQIKPRFGDFGGRIGVAVSGLIDRRTNRVALSRHTPEYVAVDLAAEVRSAT
                     GLQVVLENDANAAAYGEFALGAGRGTHNIFYVLLGTGIGGAFIIEGKLWRGGSGFAGE
                     FGHILVDSEEDLRLEDVANSANILRRIKHRVHQDSTSSLVRIRED"
     gene            856..1602
                     /locus_tag="VGB68_18505"
     CDS             856..1602
                     /locus_tag="VGB68_18505"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEY0051294.1"
                     /translation="MEQISQIIETYGIYAVFALCTVEGDITLLLSGVMAHSNFFGSYS
                     FLKVFFAGAIGGVVGDTFGYCVGRFSRHTVKDYAFYKMTQPRIERLIEKFGGFAIILS
                     KYIYGIRVAMCISSGVGRMPFLRFLAMDAISCSIWAFILAGLGYFFSGAITNIIGDFH
                     QIGIGLFFIVLTGVIIFYAIERFYLSQKVEAVKPETIHKIEEKIHAVEEVAQEKLHDL
                     GERLHLTSSPNREEKEKEKTKGKTKEKSSS"
     gene            1809..2816
                     /locus_tag="VGB68_18510"
     CDS             1809..2816
                     /locus_tag="VGB68_18510"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051295.1"
                     /translation="MIIEASAPTRVDLAGGTIDIPPLFLFHEGAATVNFAVDLMARCR
                     LETRDDDKIFIESIDRKEAFETSLANLHQLKDEPRLELLSKLVYFFKPETGFNMITES
                     EAPAGAGLAGSSTLNIACIGALNKLVGDRYAPEKFIPIAAAVECQVIKVPTGYQDYYS
                     AQYGSVSAIHFRPDGMHREALEIDTATLESRMVVIYTEEPRNSGTNNWDITKRHIDGD
                     REIFNIFEGIRDTSLNLREALLKSDWDTVGEILKKAFPHRKRLSPNITTPHMDDLIET
                     AMSSGAIAPKVCGAGGGGCIAFFCEDGKRGDVEKALSAAAGAEVLQWKLSKKGLVIKE
                     S"
     gene            complement(2904..3605)
                     /gene="purQ"
                     /locus_tag="VGB68_18515"
     CDS             complement(2904..3605)
                     /gene="purQ"
                     /locus_tag="VGB68_18515"
                     /inference="COORDINATES: protein motif:HMM:NF002957.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEY0051296.1"
                     /translation="MKFGVIVFPGSNCDHDAYHVVSKHVGQPVDFVWHKQTDLSGYDA
                     VIVPGGFSYGDYLRAGALARFSPVMNAVKDFAARGKFVFGICNGFQILCEAGLLPGAL
                     IRNANLHFICKHVNIKVENQNTPYTSELEAGEVLSIPVGHAEGNYVCDDATFDALEEN
                     NQIVFRYCNEAGEITDEANPNGARSNIAGICNQDRNVLGMMPHPERACEELLGSNDGR
                     DIFRSLTNTISKLGN"
BASE COUNT          907 a          922 c          959 g          879 t
ORIGIN      
        1 gtcttccctg attctgacca gcgaagaagt gctgtcctga tgaacgcgat gtttgatgcg
       61 gcgcagaata ttagccgaat tggcgacgtc ttcgagccgc aaatcttctt cggaatcgac
      121 cagaatatgc ccgaattcgc ccgcgaaacc cgaaccgccg cgccagagct tgccttcgat
      181 gataaacgcg ccgccgatgc ctgtgccgag cagaacatag aaaatattat gcgttccgcg
      241 tccggcgccg agcgcgaatt cgccgtaggc ggcggcgttg gcgtcgtttt ccagaacgac
      301 ctgcagcccg gtggcgctgc gaacttcggc ggcgaggtcg accgcgacgt attcgggcgt
      361 gtggcgcgac agcgcgacgc gattcgtgcg gcggtcaatc agaccggaaa cggcgacgcc
      421 gatgcgcccg ccgaaatcgc cgaagcgcgg ctttatttga ttaataaaag cgatgagttg
      481 tgatgtgacg ctctcgcggc ggtctatcgg ggcttcctgt aaatcgacgg cttcgcccgc
      541 ttgattaaga caaacggctc ttaataccag gtttgaaact tcgaccccga cgagttttcc
      601 ggcttgctcg ctcattttgc tcccttgttt cgtgaacctt gcttgatacc gcttcaggca
      661 ggaaacaatc ggaattgtat gaaagtcaca aagtaactgt caaataagcg attaagttat
      721 ttttccatta ttcggaaatg cttcccgcgc gaaaaaaaat gaaaatctta ataattaaaa
      781 acgtttgact caaaggctcg cagaaagtta ctcttttgat gagacgcgca aattgaaaaa
      841 agagtcactg gcacgatgga acagatttcg caaatcatcg aaacttacgg catttatgcg
      901 gtgttcgccc tgtgcacggt cgaaggcgat attaccctgc tgctgtcggg cgtgatggcg
      961 cacagcaatt ttttcggctc atacagtttt ttgaaagtgt ttttcgccgg agccattggc
     1021 ggcgtggtcg gcgatacgtt cggctactgc gtcgggcgtt tctcgcgcca cacggtcaaa
     1081 gattacgcct tttataaaat gacgcagccg cgcatcgagc gcctgattga aaaattcggc
     1141 ggctttgcga ttatcttatc caaatacatc tacggcatcc gcgtggcgat gtgcatttcg
     1201 agcggcgtcg gacgaatgcc cttcctgcgc tttctggcga tggacgccat cagctgttcg
     1261 atttgggcgt ttatcctggc gggtttggga tatttcttta gcggcgcaat cacgaacatc
     1321 atcggcgatt ttcatcaaat cggcatcgga ttgtttttta tagtcctgac gggcgtcatc
     1381 attttctacg cgattgagcg gttttatctc tcgcaaaaag tcgaggcggt caaaccggaa
     1441 accattcata aaatcgaaga aaaaatccac gccgtagaag aagtcgcgca ggaaaagctg
     1501 cacgacctcg gcgagcgcct gcacctgacc agctcgccga accgcgaaga aaaagaaaaa
     1561 gaaaaaacca agggaaaaac caaggaaaaa agcagcagtt aatgaaaaac gtaaataatg
     1621 caaaacgcaa aatgaaggaa cagatttaat cctgaatcct gaatcctgaa tcccgaatcc
     1681 tgaatcccga atcttaaatc cgaatatttt gcattttgca ctttgcattt tggattaatt
     1741 aaagtatttt ctgatttctg attctaatca gtaattttaa atccgtaata actttctgaa
     1801 gtaaatttat gattatcgaa gcatccgcgc caacgcgggt tgaccttgca ggcgggacga
     1861 ttgatattcc gccgcttttt ttgtttcacg aaggcgcggc gaccgtcaat ttcgccgtcg
     1921 atttgatggc gcgctgccgg ctggaaacgc gcgacgacga taaaattttt atcgaatcca
     1981 tcgaccgcaa ggaagccttt gaaacatcgc tcgccaatct tcatcaatta aaagacgagc
     2041 cgcggctgga actgctttcc aagctcgtct attttttcaa gccggagacg ggttttaata
     2101 tgattaccga atcggaagcg ccggcgggcg ccgggctggc gggttcttcg acgctgaata
     2161 tcgcctgtat cggcgcgtta aataaactgg tcggcgatcg ctacgcgccc gaaaaattta
     2221 tcccgattgc cgcggcggtc gagtgtcagg tcatcaaagt tccgaccggc taccaggatt
     2281 attattcggc gcaatacggc tcggtttcgg cgattcactt tcgcccagac ggaatgcacc
     2341 gcgaggcgct ggaaatcgac accgccacgc tcgaaagccg gatggtcgtc atttacacgg
     2401 aagagccgcg caactccggc acgaacaact gggacatcac gaagcgccac atcgacggcg
     2461 accgcgagat ttttaatatc tttgaaggca tccgcgacac atcgctgaat ttgcgcgaag
     2521 cattgctgaa aagcgattgg gatacggtcg gcgagatttt gaaaaaggcg tttccgcatc
     2581 gcaaacgcct ttcgccgaac atcacgacgc cgcatatgga cgatttgatt gaaacggcaa
     2641 tgtcgagcgg cgcgattgcg ccgaaagtct gcggcgcggg cggcggcggc tgcatcgctt
     2701 ttttctgcga agacggaaaa cgcggcgacg tcgagaaagc tctgtccgca gccgcgggcg
     2761 cggaagtttt gcagtggaag ctttctaaaa agggcttagt tattaaagag agttaaagaa
     2821 tcagggaaat taaggaaatc aggtgattag gaaatgcctt ttttcatttc ctaatcacct
     2881 gatttcctta atttccccat tccctaattt cccaatttac taatcgtatt ggtcagcgag
     2941 cgaaaaatat cgcgcccgtc gtttgagcct aataattctt cgcaggcgcg ctccgggtgc
     3001 ggcatcattc ccagaacgtt tctgtcctga ttgcagattc cggcgatgtt cgagcgcgcg
     3061 ccgttcgggt tggcttcgtc cgtgatttcg ccggcttcgt tgcagtagcg gaaaacgatt
     3121 tgattgtttt cttccagcgc gtcgaaagtc gcgtcgtcgc agacgtaatt gccttcggcg
     3181 tgcccgaccg gaatcgaaag cacttcgccc gcttccagct cgctcgtgta aggcgtgttt
     3241 tgattttcga ccttgatgtt gacgtgcttg cagataaaat gcagattcgc gttgcgaatc
     3301 agcgcgccgg gcagcaatcc cgcttcgcac aaaatctgaa agccgttgca gatgccgaaa
     3361 acaaattttc cgcgcgcggc aaaatctttg accgcgttca tcacgggcga aaaccgcgcc
     3421 agcgccccgg cgcgcaagta gtcgccgtag gaaaatccgc cgggcacgat aacggcatcg
     3481 taaccgctca aatcggtttg cttgtgccag acgaaatcaa ccggctgacc gacgtgtttc
     3541 gagacgacgt gataagcgtc gtggtcgcag ttcgagccgg gaaaaacgat aactccaaat
     3601 ttcataaata gtggtaagtg gtaagtggta agtggtaagt ggtaagcagc aaaaagaact
     3661 gtttctt
//
LOCUS       DASWNE010000950         5777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_209948, whole genome shotgun sequence.
ACCESSION   DASWNE010000950 DASWNE010000000
VERSION     DASWNE010000950.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5777
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209948"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VGB68_18520"
     CDS             <1..207
                     /locus_tag="VGB68_18520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051297.1"
                     /translation="NGLLRRFTRKKSAARKILRAALFFRVNLLNKPFAPESVLFFIKA
                     QKRLFMRQKGMELSESGQSESCRG"
     gene            384..857
                     /locus_tag="VGB68_18525"
     CDS             384..857
                     /locus_tag="VGB68_18525"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY0051298.1"
                     /translation="MNTVEKSIVVNVPVSTAYNQWTQFEEFPQFMEGVESVRQEGDSH
                     THWRANIAGKDVEWDADITEQTPDQRVSWRSTTGAHNAGTVSFQPAGAEQTNVTLRLE
                     YEPEGFFENIGSALGFVDSRVEGDLERFKTFIESRGNETGAWRGEIHGSQVENKG"
     gene            988..1713
                     /locus_tag="VGB68_18530"
     CDS             988..1713
                     /locus_tag="VGB68_18530"
                     /inference="COORDINATES: protein motif:HMM:NF018949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HEY0051299.1"
                     /translation="MFKSIVKIAAATVLFAGIHSLLASGTAKRKAAALLGDQRRNALY
                     RPFYNVLAVVTFGALMLYGAKLPDRRLYRIRAPFARLMQLIQLYFLLFLLNGARQIGF
                     LNFAGLPNIALWLRGRSPVPSEPEAQGPALGDDGRMKIAGPFRLTRHPLNFGMLPILW
                     LMPKMTVNLAVFNLITTLYLIVGSVHEEKRLKESYGRAYEDYRESGINFFVPPAGYLL
                     SRRKKELKPAGSNEFAGKNFDSQ"
     gene            1953..4346
                     /locus_tag="VGB68_18535"
     CDS             1953..4346
                     /locus_tag="VGB68_18535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY0051300.1"
                     /translation="MKSEETIIDLFGKMPYKPLVEEIKDFAIFYLDEEGRVLSWNPGA
                     EIIFRYSADEIIGQDFSILFTREDRAQGIHIKELEKARETGRADDTRWHLRQDDSLFF
                     ASGATTAIRSEDGTLLGYAKIARDETERRQIEEAFRESEERYRLLAETASDAIISIDA
                     RSIILFVNRAAERIFGYKIDEMLGQSLSMLMPEYLRHAHQTGIERYIKTGERHLSWEH
                     VEVAGLHRSGHEFPLELSFGEYKKSGERFFIGIARDITLRRRVQSEREQLLQREQAAR
                     REATNILERITDGFISVDRDFRYTYINRAGATMAGLAPEDFIGKTLWEVNPDAVGTKF
                     QIEYERALRENIPVSFTEYLSWVDLWLEVNAYPSETGLSVFFRDVTLKVRLEEERAKL
                     LEREQEARFQAEESNRIKDEFLATLSHELRTPLNSILGWSQILQTRNLDAGQALKALS
                     TIQRNARAQTQLIEDLLDVSRIITGKLSLKMCPVDLRNIIAAAADAARPAADAKEIAL
                     QTLFAEQAALISGDADRLQQVVWNLLSNAVKFTPGGGSILVSLEKAGDRHLEIIVSDT
                     GKGISEEFVPYVFERFRQSDGSMTRRHGGLGLGLSIVRQIVELHGGAVSVESAGEDRG
                     ATFRVKLPLLQIQAGNNSEIRQSSPSVEAQALSDCPPELSGLRVLIVDDEPDSLDLLN
                     VILDSCGVTTTAASSAAEAFELIQRERFDVIISDVGMPEEDGFALMSRIRELPAEKGG
                     KVPAIALTAYAREEDRKQAILSGFQTHLSKPFEPSELVSIVAKQAGRLKNSDRKEND"
     gene            4993..>5777
                     /locus_tag="VGB68_18540"
     CDS             4993..>5777
                     /locus_tag="VGB68_18540"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0051301.1"
                     /translation="MNIWQIIGRLFPFVKPYKFLVVGSVFLTLIGALAAQVNPIVLRY
                     TVDTIETLLREGRTAVGSSSLLFFISAVLFGKELVNIAVKYGQSMLGERIRINLSSRL
                     SQYAVEKILTYRYAYFADDENATGKLQTRLDRGAESLTRFVQRIFIDLMPLFANAALA
                     LVVMFSANFYVGLIASVIMPVYFLLSWRQAGLLKGVRRGLRTLRETKTNGLFNIIESI
                     IVIKSFVREAYEREKQAALQQNLIDAQLKQRKTNYSFDALKTFA"
BASE COUNT         1540 a         1386 c         1415 g         1436 t
ORIGIN      
        1 aatggcttat taaggagatt tacccgaaaa aaaagcgcgg ctcgcaaaat tttgcgagcc
       61 gcgctttttt ttcgggtaaa tctccttaat aagccatttg ccccggaaag cgttcttttt
      121 tttataaagg ctcaaaaaag actctttatg agacaaaaag gaatggagct atcggaatca
      181 ggtcaatcgg aatcgtgccg cggttgaatc aggcgggcag atttacgtta aaaactgaat
      241 ccgtatcttt atttaagacg aataaaagat atactggata aatcttcagc ctataattca
      301 cacttatgat attcataaga cctgaataaa atcggctgag tttaaggtaa tttaagaaca
      361 ataacaaggg aaggagagtt caaatgaata cagttgaaaa atcaatcgtc gtgaacgtac
      421 cggtcagtac ggcttacaat caatggacac aattcgagga atttcctcag tttatggagg
      481 gcgtcgaatc ggttcggcag gaaggtgaca gtcacactca ttggcgcgcc aatattgccg
      541 ggaaagatgt cgaatgggat gcggatatta ccgagcagac gcccgaccag agagtatcgt
      601 ggagaagcac gaccggcgcg cataatgccg gaacggtcag cttccaaccg gcgggcgcag
      661 aacagacgaa cgtaactttg cgactggaat acgagccgga aggatttttt gagaatatcg
      721 gcagcgcgct cggatttgtc gactccagag ttgagggcga tctggaaaga ttcaaaacat
      781 ttattgaaag ccgcggcaat gaaaccggag cttggcgcgg cgaaattcac ggctctcaag
      841 tcgaaaacaa aggttaatca aacaaaaaca taaacaaaaa gagagtgttg attcactctc
      901 tttttgttta tgtaattcgg ctttttgccg gtaatcggat tgcgttttgc caaatggcga
      961 taattgtctt gaatataaaa tttagacatg tttaaaagca ttgtaaaaat tgccgccgcg
     1021 accgttctgt ttgccggaat tcacagcttg ctggcatccg gtacggcaaa aagaaaggca
     1081 gccgctctac tcggcgacca gcggcggaac gctctctatc gaccattcta caatgtgctg
     1141 gctgtcgtca ctttcggggc gttaatgctc tacggcgcga aattgcccga ccgccggctg
     1201 taccggattc gcgctccatt cgcccgattg atgcagctta tccaacttta ttttttgctt
     1261 tttttgttaa acggcgcgcg acaaatcggc ttcttaaatt tcgcgggatt accgaatatc
     1321 gccttatggc tgagaggtcg ctcgccggtt ccttcggaac ctgaagctca aggtcccgct
     1381 ctcggcgacg acggtcgtat gaaaattgcc ggtccgtttc gtttaacacg gcatccgcta
     1441 aatttcggga tgctgccgat tctctggctg atgccgaaaa tgaccgtcaa tctcgccgtt
     1501 tttaatttga taaccacgct gtatttaatc gtcggctcgg ttcacgagga aaaacgcctg
     1561 aaagaatctt acggtcgcgc ttatgaagat taccgcgaaa gcggaatcaa tttctttgtt
     1621 cctccggcag gctatttact ttcccggaga aagaaagagt taaagccagc cgggagcaat
     1681 gaatttgcgg gcaaaaactt tgattctcaa taaaaacttt gattctcaat agagagataa
     1741 aaataccgct ttttctttta cccgcgcttt ttccgcttgc aaaaatgccg actgacgggc
     1801 aaacttttcc agcccaaata taatccgccg gctttcttcc agtattaatt atttcggctc
     1861 gcctctgtcg gtttccccaa actaaacgcg aatattttat tcccaatcat catttaaaat
     1921 agtatgttac taataataca acttatttga agatgaaatc agaagaaaca attatagacc
     1981 ttttcggtaa aatgccttac aaacctctgg ttgaggaaat caaggatttt gccatttttt
     2041 acctcgacga agaaggtcgc gttttaagct ggaatccggg cgccgagata atcttccggt
     2101 acagcgcaga tgaaatcatc ggacaggatt tctctattct ctttacgcgg gaagacaggg
     2161 cgcagggcat acatataaag gagctggaaa aggctcggga gacggggcgc gccgacgaca
     2221 cgcggtggca cttgagacag gacgattccc tttttttcgc ttccggcgct actaccgcca
     2281 ttcgcagcga agacggaacg ctcctcggct atgccaagat tgcccgcgac gagaccgagc
     2341 gcagacaaat agaagaagcc tttcgtgaaa gtgaagaaag atatcgcctc cttgccgaaa
     2401 ccgcctcgga cgcaatcatc agtatcgacg cgcgatcaat tattttattc gttaatcgag
     2461 ccgcggaaag aatcttcggt tacaaaatcg acgagatgtt aggtcagtcc ctgtcgatgc
     2521 tgatgcccga atacttacgt cacgcgcatc agacaggaat cgagcgctac ataaaaacag
     2581 gtgagcgtca tctttcctgg gagcacgttg aagtcgccgg gctgcaccgc agcggtcacg
     2641 aatttccgct tgaattatcc ttcggagaat acaagaaaag cggcgagcgt ttctttatcg
     2701 gtattgcccg cgacattacg ctgcgcaggc gagtgcaatc cgaacgagaa caacttttgc
     2761 agcgcgaaca ggcggcgcgc agggaagcga ccaatattct cgaaagaatc acggacggct
     2821 ttatttccgt cgaccgcgat tttcgttaca cctatatcaa ccgcgccgga gcgacgatgg
     2881 ctggtctggc tccggaagac tttatcggaa aaacgctctg ggaagtaaat ccggacgcgg
     2941 tcggaacaaa atttcagatt gaatatgagc gggcgctccg tgaaaatatt cccgtctctt
     3001 ttaccgaata tttgtcgtgg gtcgatcttt ggcttgaagt aaacgcttat ccgtccgaga
     3061 cgggtctttc ggttttcttc cgcgacgtga cgcttaaagt ccggctggaa gaagagcgcg
     3121 ccaagcttct cgagcgcgaa caggaagcgc gttttcaggc tgaggaatcc aatcgcatca
     3181 aagacgaatt tctcgctacg ctctcgcacg aattacggac gccgctgaac tcaattctgg
     3241 gatggtctca aatattgcaa actcgtaatc tcgatgccgg gcaggcgtta aaagcattat
     3301 ccaccatcca gcgcaatgcc cgggcgcaga cacagttaat cgaggattta ctcgacgtct
     3361 ccagaattat taccggaaaa ctcagtctta agatgtgccc ggtcgatttg agaaacatta
     3421 tcgcggcggc agccgacgcc gcccgaccag ccgcagatgc caaagaaatc gctctgcaaa
     3481 cgctttttgc cgaacaagcc gcactgatat cgggagatgc cgatcggcta caacaagtcg
     3541 tgtggaatct actgtcgaac gccgtaaagt ttacgcccgg cggcgggagc attctggtca
     3601 gccttgaaaa agccggcgac cggcatctgg aaatcattgt cagcgacacc ggcaagggaa
     3661 tctcggaaga gtttgtgcct tacgtctttg aacgctttcg tcagtccgac ggctcgatga
     3721 cccgtcgtca cggaggcttg ggcttgggct tgtcaattgt tcggcaaata gtcgagctgc
     3781 acggcggagc cgtctcggtt gaaagtgcgg gcgaagatag gggcgcaacc ttcagggtca
     3841 aattgccgct gcttcaaatt caagccggta acaacagtga aatacgacaa tcaagcccga
     3901 gcgtcgaagc acaggcgctt tccgattgtc cgcccgagct ttccggcttg cgggtgttaa
     3961 ttgtggacga cgaaccggac tcgctggatt tgctgaatgt aatcctcgat tcgtgcggcg
     4021 tgacgacgac ggcggcaagc agcgcagccg aagcattcga actaattcag cgagaacgtt
     4081 tcgacgtaat catcagcgat gtcggaatgc ccgaagaaga cgggtttgcc ctgatgagca
     4141 gaattagaga attaccggct gaaaagggcg gcaaagtgcc ggcaatcgca ttaaccgctt
     4201 atgcccgcga agaagatcga aaacaagcca tactttcagg tttccagacg catctctcga
     4261 aaccgttcga accttcggag cttgtcagta tcgtggcaaa acaggcaggg cggttgaaga
     4321 attccgaccg gaaagaaaat gattaaagaa ttcagcccgg gacgaaagga ttccgaaagc
     4381 agccttttcc tgtttagcgc cgccgtttta tctctttcgc ttatactcga taaaagattt
     4441 tacagtctgc ctttcatcgt tttagcaaca atcgtcgaca tcgaacagcc ggtacgaggc
     4501 tttgcacaaa aataaagaaa gggaaaattg taaattccaa attgtaaatt ctaaattgat
     4561 gttcggtatc ttttgtgaag taattgagaa ttcataattg agaatttaca attagccatc
     4621 ggaatttttc tcgatttcct ttatttttgt gcaaagcagt ttcgatgcgc ttttaatcaa
     4681 acgaactcgc aaacgacaag gtctgctcga caaagcaaat cgtcacgcgg gacgccggag
     4741 ccgggcattt gcagtttttg ctttccaatg aatgatttta ttcattctaa actgaaagaa
     4801 taaaggctgc gcggcgcttg accgtcattc tttcgggtcg ataaaaacag aacagccggt
     4861 gcggttaaaa tattttaacc gcaccggctg ttttttattc atttataaaa gccgaatttt
     4921 ctttatttgt ccggctcgac cgtttgaatt cttttgcgtc cgcctacgaa atcaaactaa
     4981 aatttataga caatgaatat ctggcagata atcggacggc tctttccctt cgtcaagcct
     5041 tacaaatttc tcgtcgtcgg ctcggtgttt ctgactttaa tcggcgcgct cgccgcgcag
     5101 gtcaacccga ttgttctgcg ctatacggtc gatacgattg aaactcttct gcgcgaaggc
     5161 agaaccgccg tcggcagctc gtcgctgctg ttttttatca gcgcggtttt gttcggcaaa
     5221 gagctcgtca atatcgccgt caaatacggg cagagtatgc tcggcgagcg cattcgcatt
     5281 aatctttcca gccgtttgtc gcaatacgcc gtcgaaaaaa ttctgaccta cagatacgct
     5341 tatttcgccg acgacgaaaa cgcgaccgga aagctgcaaa ccagactcga ccgcggcgcg
     5401 gaaagcctga ccagattcgt gcagagaatt tttatcgatt tgatgccgct tttcgccaac
     5461 gccgcgctgg cgctcgtcgt gatgttttcc gccaattttt atgtcggttt aatcgcctcg
     5521 gtgattatgc cggtttattt ccttttaagc tggcggcaag ccgggttatt aaaaggcgtg
     5581 cggcgcgggc ttcggacgct gcgcgagacg aagacgaacg ggcttttcaa catcatcgaa
     5641 tcaatcatcg tcatcaaatc ctttgtccgc gaagcatacg agcgcgaaaa acaagccgca
     5701 ttacagcaaa acctgattga cgcgcaatta aagcagcgaa aaaccaatta ttccttcgac
     5761 gcgctcaaga cctttgc
//
LOCUS       DASWNE010000951         2795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_279509, whole genome shotgun sequence.
ACCESSION   DASWNE010000951 DASWNE010000000
VERSION     DASWNE010000951.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279509"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..>2795)
                     /locus_tag="VGB68_18545"
     CDS             complement(13..>2795)
                     /locus_tag="VGB68_18545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051302.1"
                     /translation="CKFFLSFFLLFILLFQIAAPAFACGPSYITPVFDYKHAPEEPFE
                     NFAAGKIGIVKTSYRRVVLLAAYRYLNGGGFSAAEQKGLVDVWNAEFNNRSFDDDDVS
                     EAVKAWVERRREAVKAEEKPPSIYTERESGGYDFFPNCTKSAFETAAQTLSDRISSYG
                     AEDKNVQAWVKAQDEVFMNCASGQAIPEPAIPEAPEWLKKDREYQIAAANFYSMNYDT
                     ARTIWTKIADDSDSVWADTADYLIARTLMREASLSKSPEKSKEIYAQAEGHLYRLTAR
                     GGKFYGASLKLLNLIKYRLHPEERVRELAGSLAVPGGNDNFRQDVIDYHWLLDKFEKQ
                     ALEAEEERKKELARQRETEKAEQNSNETNANSASNTNSSASTSNISSSNTIYTGNTSS
                     NAAINQTPLEPITSEQVEAGEALGFGLTLSEGSYRYFRFLVSQTDEEIFAEVEQDIGR
                     KLTDKEKGIVQEARSASYRSKTNNQPYNAYEGGYYGSEETTLALLPDFLRQDDLTDWL
                     FTYQTGDEAAYRHALSRWEQSKSDLWLTTALAKAEANSNDLKRLLAEAERIDRNAPAY
                     ATVAYHAARLYIGQKKFAEARRVLDEILNSTLDLPVSTRNQFLRQRMSLAETLDEFLK
                     LAQRRPFGFDFDGSTGTIEQFIAEQKSWYDPKYNTEQTREEYEREVEDRLKEEKEWQS
                     RAMFDEDAVEIINQHFPLAVLLEARKSPALPDYLQKRLALAIWVRAVLLDNDAVARKI
                     SPEVIKFAPELTPLWMPYQNASTPLERKRAALFLILKNPILTPLIESGMGKTDNETSE
                     FDSDDWWCTPYDSEYDDSGANVGFRLPARPAFLTGAQSDAAQAERAKLKQLGDAPRFL
                     GDTVLEWQRLAPADKRVPEMLYIAYKANGWTKYGCGNNEELRKNIAQALKTRYPKSKW
                     TAKMIEEETGNN"
BASE COUNT          555 a          763 c          718 g          759 t
ORIGIN      
        1 aactatcagt tatcagttat ttcccgtttc ctcttcaatc attttcgccg tccatttgct
       61 tttcgggtag cgggttttca gcgcctgcgc aatatttttt cttaattcct cgttgtttcc
      121 gcagccgtat tttgtccagc cgtttgcctt gtaggcgata tacaacattt cgggaacgcg
      181 tttatcagcc ggagcgagtc tctgccattc cagaaccgtg tcgcctaaaa atcgcggagc
      241 gtcgccgagc tgcttcagct ttgcgcgttc ggcttgcgcc gcgtcgctct gcgctccggt
      301 cagaaacgcc ggtcgcgcgg gaagccggaa tccgacattc gcgccgctgt cgtcatattc
      361 ggaatcatac ggcgtgcacc accaatcgtc cgaatcaaac tcgctggttt cgttatcggt
      421 tttgcccata ccgctttcga taagcggcgt taaaatcgga tttttcagaa ttaaaaacaa
      481 cgcggcgcgt tttctttcga gcggcgtcga agcgttttga tacggcatcc agagcggcgt
      541 caattccggc gcgaatttga tgacttccgg cgagattttt ctcgcgacgg catcgttatc
      601 gagcaaaacc gcgcgcaccc aaatcgccag agccagacgt ttttgcagat aatccggcag
      661 cgcgggcgat ttccgggctt cgagcaaaac cgcgagcgga aaatgctggt tgatgatttc
      721 caccgcgtct tcgtcaaaca tcgcgcggct ttgccattct ttttcctctt tcaggcggtc
      781 ttcgacttcg cgctcgtatt cttcgcgcgt ctgttcggtg ttgtatttcg ggtcgtacca
      841 ggatttttgc tcggcgataa actgttcaat cgtgccggtc gagccgtcaa aatcgaaacc
      901 gaaaggtcgg cgctgcgcga gtttcaaaaa ttcgtcgagc gtttccgcca gcgacattct
      961 ttgccggaga aattgattgc gcgtcgaaac cggcaaatca agcgtcgaat ttaaaatctc
     1021 gtcgagcact cttctggctt cggcaaattt tttttgcccg atgtaaagcc tcgccgcgtg
     1081 ataagcgacg gtcgcgtaag ccggcgcgtt gcggtcgatt ctttccgctt cggcgagaag
     1141 tcgtttcaaa tcgttcgagt ttgcttcggc tttggcgagc gccgtcgtca gccacaaatc
     1201 ggatttgctc tgctcccagc gcgaaagcgc gtgccggtaa gcggcttcgt ccccggtttg
     1261 ataggtgaaa agccagtcgg tcaaatcgtc ctgccgcaaa aaatcgggca aaagcgcgag
     1321 cgtggtctct tccgagccgt aataaccgcc ttcgtatgcg ttgtagggct gattattggt
     1381 tttgctgcga tagctcgcgc tccgcgcttc ctgaacgatt cctttttcct tgtcggtcag
     1441 ttttctgcct atatcctgct cgacttccgc gaaaatttct tcgtcggtct gcgagactaa
     1501 gaatctgaaa taacggtaac tgccctcgct cagcgttaaa ccgaaaccca aagcctcacc
     1561 cgcctcaacc tgctccgaag taatcggctc gagcggagtt tgatttatcg ccgcgttcga
     1621 gcttgtattt cccgtgtaaa tggtgttgct gctgctgata ttgcttgtcg aggcgctcga
     1681 atttgtgttc gatgccgaat tcgcattcgt ttcgttgcta ttctgttccg ccttttcggt
     1741 ttctcgctgg cgcgcaagct cttttttacg ttcttcttcg gcttcgagag cctgcttttc
     1801 aaatttatcc agcagccagt gataatcaat gacgtcctgc cgaaaattat catttccgcc
     1861 cgggacggcg agcgagccgg ctaattcgcg gacgcgctct tcgggatgca ggcgatattt
     1921 gattaaattc agaagcttca gcgatgcgcc gtaaaacttt ccgccgcgcg ccgtcagccg
     1981 gtaaagatgt ccttcggctt gcgcgtaaat ttctttcgat ttttccggac ttttcgatag
     2041 gctcgcctcg cgcatcagcg ttcgcgcaat caggtaatca gccgtgtccg cccagaccga
     2101 atcggaatcg tcggcgattt tcgtccagat tgtgcgcgcc gtatcgtaat tcatcgaata
     2161 aaaattagcg gcggcgattt gatactcgcg gtcttttttg agccattcgg gcgcttccgg
     2221 aatcgccggt tcgggaatcg cctgaccgct ggcgcagttc ataaaaactt cgtcctgcgc
     2281 tttgacccag gcttggacgt ttttgtcttc cgcgccgtag cttgaaattc tgtcggagag
     2341 cgtctgcgcc gcggtttcaa acgcggattt cgtgcagttc gggaaaaaat cgtagccgcc
     2401 cgattcgcgc tcggtgtaga ttgacggtgg tttttcctcg gctttcaccg cttccctgcg
     2461 tctttcgacc caggctttga ccgcttccga aacgtcgtcg tcgtcaaagc tccggttgtt
     2521 aaattcggcg ttccagacat cgaccagacc tttttgctcg gcggctgaaa atccgccgcc
     2581 gtttaaataa cgatacgccg ccaaaagcac gacgcggcga tacgaggttt tgacgatgcc
     2641 gattttgccc gccgcaaaat tttcaaacgg ttcttcgggc gcgtgtttgt aatcgaaaac
     2701 cggcgtgata tagctcggac cgcaggcaaa agcgggcgcg gcgatttgaa aaagcaaaat
     2761 aaacagaaga aagaaagaca gaaaaaattt acacg
//
LOCUS       DASWNE010000952         3309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_342912, whole genome shotgun sequence.
ACCESSION   DASWNE010000952 DASWNE010000000
VERSION     DASWNE010000952.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342912"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VGB68_18550"
     CDS             complement(<1..211)
                     /locus_tag="VGB68_18550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051303.1"
                     /translation="MPTDNLPVISFESSAEWEEWLAVNHARSTGVWIRMFKKATGIAS
                     ITYAEALNEALCYGWIDGQKNKYDDE"
     gene            439..831
                     /locus_tag="VGB68_18555"
     CDS             439..831
                     /locus_tag="VGB68_18555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051304.1"
                     /translation="MSYLRKFGLVVSAIAFCLSFYVASADAQWRQNRNWENRERSIYS
                     DRNTNRRWNSSRNVYRGQNRGGYWQQNRYGRLTPREYWRLQRQRSRIYRSADRYSRDG
                     YLSSSERRRLMRQQNRYRRNVYRQSRDW"
     gene            complement(1011..1286)
                     /locus_tag="VGB68_18560"
     CDS             complement(1011..1286)
                     /locus_tag="VGB68_18560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051305.1"
                     /translation="MDSDNQEALRHDDGTNKILEAIGDLRREMNERLDSMDRRLFKLE
                     KEVSEMKDLQLTFDVRLDRLESMAHESLQIAYGVRADVKVLRAEVAA"
     gene            1418..2695
                     /locus_tag="VGB68_18565"
     CDS             1418..2695
                     /locus_tag="VGB68_18565"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEY0051306.1"
                     /translation="MKKIICLFLLFAFGVPVFAQTMPAGADAALWQRALKIHKKAIIV
                     DGHNDIPSPLVDDDFDLSTNSVGRFHRDGDPFHTDLARFKASGITGEFFSIYVSGADY
                     EKGGKMRRAMDLIDATMREVEKHPAQLTACNTAAQIRRAKKQNKVCVLMGIEGGYVIE
                     NSLYALRNFYRLGIRYMTLTHNVSHDWADAHRGEVKNNGLSDFGKEVVREMNRLGMLV
                     DISHVSDKVMNDVLDVTTAPIIASHSSARGVSNHTRNVPDDVLKRVARNGGVIMINFY
                     PSFLDERTNREENERSARLKPQLDALRERYKNDRQAYNEAERKLMAENPIYVASYTRI
                     VDHIDHIKKVAGIDCVGIGSDYDGVPFLPAPMNGMEDLVLVTYEMLRRGYSELEVRKV
                     LGENFLRAFARAEQTAKIDSRRISADGSLKKIK"
     gene            2696..>3309
                     /locus_tag="VGB68_18570"
     CDS             2696..>3309
                     /locus_tag="VGB68_18570"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0051307.1"
                     /translation="MQFCHICGGDLIPRFADVADAQTGEKFSVFACGRCGAAQTFPTP
                     EKLDKYYVDYHGRRHGFTADYCARRRLRWLEKSFDSGKKAKRLLDVGCGEGTFLAAAK
                     KHGWQAVGTETVAENFRDSDLEVFSDFAGVKAKYGAESFDAITMWHTLEHFQMPREAL
                     RQVFELLAPKGVLLVAVPDARGWQALASGKYWLHLDVPRHLFHFG"
BASE COUNT          784 a          867 c          865 g          793 t
ORIGIN      
        1 actcgtcgtc atatttgttt ttctgaccgt caatccaccc gtagcataac gcttcgttga
       61 gcgcttcggc gtaggtgatg gaagctattc ccgtcgcttt tttgaacatc cgaatccaga
      121 cgccggtcga tcgggcgtga ttgacggcga gccattcttc ccattcggca cttgattcaa
      181 atgaaataac cggtaaattg tctgtcggca tatgtatgtt aagtataaga ttatagtggc
      241 ttttacttca atataaggaa accgtctcga ttcgcggcag agcccggatt gaaacaaagc
      301 attttttcga tttctttttt tgacaacccg accgttaaaa ctttcatcac tcaaacacag
      361 catctttgct aaatcggaat tctttcgttc ttcggatggc ggaattccat aaatttttga
      421 acgaacaagg agaggaatat gagttatttg agaaaattcg gtctggtcgt ttcggcaatc
      481 gccttttgtt tgagcttcta cgtcgcttcg gcggacgctc agtggagaca gaacaggaat
      541 tgggaaaaca gggagcgcag catttacagc gaccgcaata cgaatcgtcg ctggaacagc
      601 agccgcaacg tttaccgcgg gcaaaatcgc ggcggttact ggcagcagaa tcgatacggg
      661 cgcttgacgc cgcgcgaata ctggcggctc cagcgccagc gctccagaat ttatcgcagc
      721 gccgaccgct attcccgtga cggttatttg agcagcagcg aacgccgccg gctgatgaga
      781 cagcaaaatc gctatcgccg caacgtttat cgtcaaagcc gcgattggta gtttacaacc
      841 gaataactct cccttgtaaa aagaacggtt tgcattttaa accgttcttt tttattttgt
      901 ttccgctctt caataaaaaa agaacagccc gcttgagagc tgttcccttt tcgccttttc
      961 agatatatcg tttaagcgac ttttctttgc aaatccgtca catcttctgc ctaggctgcg
     1021 acttccgccc gcagaacctt cacgtcggcg cgaacgccgt aagcaatctg caaggattcg
     1081 tgcgccattg attccagacg gtcgagacgg acatcgaaag tcagctgtaa atccttcatt
     1141 tcactcactt ccttttccag tttgaacaaa cgcctgtcca ttgaatcaag tcgctcattc
     1201 atttcccgcc gcaaatcgcc gattgcttcg agaatcttat tcgtgccgtc atcgtgtcgt
     1261 aaagcttcct gattatccga gtccattcta atttctccgc cgcctgaaaa ttctaacata
     1321 gaaacgcgcg cccaaaacaa aatgttgcgg tcattctcgc caaattttcg gaaaaacgct
     1381 aaacttatcg caaatctttc cccttgttcc gaagcttatg aaaaaaatta tctgtctgtt
     1441 tctgcttttc gctttcggcg ttcccgtttt tgcccagacg atgccggcgg gcgctgatgc
     1501 ggcgctctgg cagagggctt taaaaattca caaaaaagcg attatcgtcg acggtcacaa
     1561 cgacattccg agcccgctgg tcgacgacga tttcgattta tcgaccaatt ccgtcggcag
     1621 atttcatcgc gacggcgacc cgtttcacac cgatttagcg cgttttaaag cgagcggcat
     1681 cacgggcgag tttttctcga tttacgtctc gggcgcggat tatgaaaagg gcggaaaaat
     1741 gcggcgggcg atggatttga ttgacgcgac gatgcgcgaa gtcgaaaaac atcccgctca
     1801 attgacggcg tgcaatacgg cggcgcaaat caggcgggcg aaaaagcaaa ataaagtctg
     1861 cgtcctgatg ggcatcgagg gcggctacgt gattgaaaat tcgctctacg ccctgcgaaa
     1921 tttttaccgg ctcggcattc gttatatgac gctgacgcac aacgtatcgc acgattgggc
     1981 ggacgcgcat cgcggcgagg tgaaaaataa cggcttgtcg gatttcggca aggaagtcgt
     2041 gcgcgagatg aaccggctgg gaatgctggt cgacatctcg cacgtctccg acaaagtgat
     2101 gaacgatgtt ttagatgtga cgaccgcgcc gattatcgcc tcgcattcgt ccgcgcgcgg
     2161 cgtctcgaac cacacgcgca atgtgccgga cgatgtttta aaacgcgtcg cgcgaaacgg
     2221 cggcgttata atgataaatt tttacccgtc gtttctcgac gaaagaacga accgcgaaga
     2281 aaacgagcgc tcggcgcgcc tgaagccgca gcttgacgcg ctgcgcgagc gatacaaaaa
     2341 cgatcgccaa gcctataacg aagccgagcg caaattgatg gcggaaaacc cgatttacgt
     2401 ggcgagctac accagaatcg tcgaccacat cgaccatata aaaaaggtcg ccggcatcga
     2461 ctgcgtcggc atcggctccg attacgacgg cgttccgttc ctgcccgcgc cgatgaacgg
     2521 tatggaagat ttggttttag tcacttatga aatgctccgg cgcggttaca gcgagctgga
     2581 agtgcgcaaa gttctgggcg aaaattttct gcgcgctttt gcccgagccg aacagacggc
     2641 gaaaatcgat tcgcgccgga tcagcgccga cggcagctta aaaaagatta aataaatgca
     2701 gttttgccac atctgcggcg gcgatttgat accgcgcttc gcggacgtcg ccgacgcgca
     2761 gacgggcgag aagttttccg tctttgcctg cggcagatgc ggcgcggcgc agacttttcc
     2821 gacgccggaa aagcttgata aatattacgt cgattatcac ggcaggcggc acggctttac
     2881 ggcggattac tgcgcccgcc gtcgattgcg ctggctggaa aaaagcttcg attccggcaa
     2941 aaaagcgaag cgcctgctcg atgtcggctg cggcgagggc acttttttgg cggcggcgaa
     3001 aaagcacggc tggcaagcgg tcggaaccga gacggtcgcc gagaatttcc gtgattcgga
     3061 tttggaggtt ttttccgatt tcgccggggt caaggcaaaa tacggagccg aatcgttcga
     3121 cgcgataacg atgtggcaca cgctcgaaca ttttcaaatg ccgcgcgaag ctctgcggca
     3181 ggtctttgag ctgctcgcgc cgaaaggcgt tttgctcgtc gccgtgccgg acgcccgcgg
     3241 ctggcaggcg ctcgcctccg gcaaatactg gctgcatctg gacgtgccgc ggcatctttt
     3301 tcatttcgg
//
LOCUS       DASWNE010000953         1693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_342946, whole genome shotgun sequence.
ACCESSION   DASWNE010000953 DASWNE010000000
VERSION     DASWNE010000953.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342946"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="VGB68_18575"
     CDS             <1..676
                     /locus_tag="VGB68_18575"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF003766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEY0051308.1"
                     /translation="IPIERLYEICEIARKILDGEDKVGRVIARPFVGETAETFKRTEN
                     RHDYAVAPPPDNLLPLLKDNGLDVVCIGKIASIYDSCGVTEDLTAKNNDQSIDQSVKA
                     LNAESRGLIFSNLVDFDMLYGHRRDTEGYARALEHFDRRLPEILDAMRADDLLIITAD
                     HGNDPTKEGSDHTREYVPLLVYGKSARAGVDLGTRASLADIGQTIAENFDLRLKDGAS
                     FLNRIL"
     gene            847..999
                     /locus_tag="VGB68_18580"
     CDS             847..999
                     /locus_tag="VGB68_18580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051309.1"
                     /translation="MANRTSDRTGEPVRKPITKATYIMIAALILFLIIAAVLIFRFLS
                     APTGNP"
     gene            complement(1267..1461)
                     /locus_tag="VGB68_18585"
     CDS             complement(1267..1461)
                     /locus_tag="VGB68_18585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051310.1"
                     /translation="MITATGEFGNAGTAPSDPFGYYRFDGLSTGSPDTFAARQKQFVF
                     SAQNYTINKNINELNFTAAP"
     gene            complement(1514..1651)
                     /locus_tag="VGB68_18590"
     CDS             complement(1514..1651)
                     /locus_tag="VGB68_18590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051311.1"
                     /translation="MLCARTLQRASLICAVPYKMAEAKGEAQLAKTARENYDRISNKM
                     K"
BASE COUNT          410 a          450 c          421 g          412 t
ORIGIN      
        1 tattccgatc gaaaggcttt acgaaatctg cgagatcgcc cgaaaaattc tcgacggcga
       61 ggacaaagtc gggcgcgtga ttgcgcgccc gttcgtcggc gaaacggcgg aaactttcaa
      121 gcgcaccgag aatcgccacg attacgccgt cgcgccgccg ccggacaatc ttctgccgct
      181 tttgaaagat aacggtttgg acgtcgtctg catcggcaaa atcgcttcga tttacgactc
      241 ctgcggcgtc accgaagatt tgaccgcgaa gaacaacgac cagtcgattg accagagcgt
      301 caaggcttta aacgccgaat cgcgcgggct gattttcagc aatctcgtcg atttcgatat
      361 gctctacggg caccggcgcg acacggaagg ctacgccaga gcgctggaac attttgaccg
      421 ccgcctgccg gaaattttag acgcgatgcg cgcggatgat ttattgatta tcaccgccga
      481 ccacggcaac gacccgacca aagaaggctc tgaccacacg cgcgaatacg tgccgctgct
      541 ggtttacgga aaatccgcgc gcgcgggcgt cgatctcgga acgcgcgcca gccttgccga
      601 catcggtcag acgattgcgg agaatttcga tttaaggtta aaagacggcg cgagtttttt
      661 gaaccggatt ctttgatacc ggaaaacgat tttcattttg ttgtatcgga attatacgcc
      721 gcttccgttt cgttccgata cgataaaaac agtcttaatt ttttaaagcc gccgccttgc
      781 ggatttttaa ataaaatcgc cgcgcggcac gtcgcttgcc gtcgaattcc ggaaaggaga
      841 aaaacaatgg caaacagaac cagcgaccga accggcgaac ccgtccgcaa acctattacc
      901 aaagctacat acataatgat tgcggcgctt attttatttc taattattgc cgccgtgctt
      961 atcttcaggt ttttaagcgc gccgaccggc aatccgtaat cgattaatca aaccccgaac
     1021 tgataaccgg cgcgcggtcg gcaaaaatta cagcgccgat taaacggcgt gagttttcct
     1081 gataaaatcc gattaaacgg acggcttgct caacagtttt tcagttcgtt taaaaaaggc
     1141 ttaatcctgg ctgcgcgccg gaacccgaaa attttcgggt tccgacgcgc aaacatcttt
     1201 ttacctatcg atgccgccgc tgcttacccg gcaaatttct tttgccgatt aattatttcc
     1261 ggcgatttac ggcgcagccg tgaaattcaa ttcatttatg tttttattga ttgtgtaatt
     1321 ctgcgcagag aaaacaaact gtttttggcg cgcggcgaaa gtgtcgggcg agccggttga
     1381 aagcccgtcg aatcggtaat agccgaacgg gtcggacggc gcggtgccgg cgtttccgaa
     1441 ttcgccggtt gccgttatca aagcgccgga aaaccggttt tccggcggag tccgtcacgc
     1501 ggttaaaatt cttttatttc atcttgtttg aaatcctatc gtaattttcc ctggcggttt
     1561 tcgccagctg cgcctcgcct ttcgcttccg ccatcttgta gggaacggcg caaatcaacg
     1621 aagccctttg caaagttctc gcgcataaca tctgcgtcct gattcaatca atgaacgaac
     1681 tggaaatcaa gcg
//
LOCUS       DASWNE010000954         4871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_67010, whole genome shotgun sequence.
ACCESSION   DASWNE010000954 DASWNE010000000
VERSION     DASWNE010000954.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67010"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="VGB68_18595"
     CDS             complement(<1..972)
                     /locus_tag="VGB68_18595"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY0051312.1"
                     /translation="MKHNFKQTARLAFLALALSLFMSAFAFGQEVTGSIVGTVRDNTG
                     AAVPGATVTITDAQKDNIVVRTITTSDDGEFSAPNLTTSVFTVTVEAQNFKKSVNTDV
                     KIDVGQRRQLDIVLQAGRIEETVTVESDAVSVDLTTPTAGTVINGDQVRELSLNNRNF
                     VQLVALAPGVSSDLADQVYVGTTNPEGQANTINLSVNGARSSQNTFTVDGADITDRGS
                     NITIQAYPSVDSIGEFRVLRSLYPAESGRSGGGQVNVVTRSGTDRFSGNFFEFIRNEA
                     FNANDFFTNQNKAAGVDENGKAKRRPFRYNNFGWTLGGPVYFFNFGER"
     gene            complement(1171..2304)
                     /locus_tag="VGB68_18600"
     CDS             complement(1171..2304)
                     /locus_tag="VGB68_18600"
                     /inference="COORDINATES: protein motif:HMM:NF018743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HEY0051313.1"
                     /translation="MTVLNGIDVLEKEKFKRLENLRIGLVTNHTGKNLSGKPTIDVLK
                     EAPNVRLVALFAPEHGIRGQLDQEKISDSTDEKTGLPIYSLYGETRKPKPEQFADLDA
                     IVFDIQDVGVRFYTYISTLKGVLEEASKAKKTVFVLDRVNPINGSTVEGALADADKLN
                     FIATHPIVVRHGMTVGELAQMFNAESKIGADVRVVKMENWSRAMWFDESGQIWTNPSP
                     NMRSLTQATLYPGVGLLEVTNVSVGRGTDTPFEVLGAPWLDGQKLALYLNRRNLPGVR
                     FVPVRFKPGASVYKDENLGGVNVIITDRKAFESVRTGIEIAVALRRLYPGEWQVDRYA
                     RLLVNAEILERVKRGDAPEEIEKAWQSQLDEFKKRRASFLLYN"
     gene            complement(2389..3816)
                     /locus_tag="VGB68_18605"
     CDS             complement(2389..3816)
                     /locus_tag="VGB68_18605"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY0051314.1"
                     /translation="MKKFVSRKSSVVGLFSIDKLQNYSLRFRVSAAMFFAFCFLPFAF
                     AKAQSLPVALPQTVGMNAAKLNQIDALVAKDIADKKLPGAVVLVGHKGKIVFRKAYGN
                     RSLTPTIEKMTIDTIFDVASLTKPVATATSVMILVEQGKLRLNDTVGRFIPEIQDENA
                     RRVTIQQLLTHVSGYAPDFDLREKWETSEGMRQALYKEKLRSAPGTRFVYSDIGFIVL
                     GEIIERIGCQDYFDCSQKSNIDPAPLNNFANSEIFSRIGLKDTIFLNDSVYSVRDKIF
                     EGNKLVKNIREDLLRTAPTENIKGQQNYLGSKFEGDEKTGNQILRGKVHDPTAFRMNG
                     VAGHAGLFSTADDLARYCQMLLNEGTLDGKRILSAQTVARMTAPVVVSEDGATRGLGW
                     DMNTSFSSNRGDLFPLGSFGHTGFTGTSVWIDRVSQTFVVFLSNRVHPDGKGDVTPLR
                     SRVATVVASAIEDAPVEAIRQAEND"
     gene            complement(3813..4751)
                     /locus_tag="VGB68_18610"
     CDS             complement(3813..4751)
                     /locus_tag="VGB68_18610"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY0051315.1"
                     /translation="MANDQSFMEIRQLRAFVAIAEAKTFTAGAKRVHVTQAAISMQIR
                     QLEDEVGLPLFTRTPRRVLMTEAGDLLLERARKILREHDAAVEEIAEIAGAEYGRLRI
                     GSASAMFTTNQLPKILQNLKAKFPNAVVSVASGTSAALVQKIMNGESDVAFVSLPVEN
                     SNIQTELLFSDEIVAIANPKHPLAKQKVISAAALADEPLILGEQGGNTRRQIDDFFLQ
                     VGVKPNVVMELSRQSAINRMVENNMGVGIASAKTVKQSVAEGKFVAWWIEGATMNWEL
                     GLARLRGGYFSPIAHEFARLCRESFAEKEKELKSKK"
BASE COUNT         1098 a         1350 c         1225 g         1198 t
ORIGIN      
        1 gcgttctccg aaattgaaaa aatataccgg accgcccaat gtccagccga agttgttgta
       61 gcgaaacggg cggcgcttgg ctttaccgtt ttcatcaacg ccggcggctt tgttttgatt
      121 cgtaaaaaag tcgttggcgt taaacgcttc gttccggata aactcaaaaa agttgccgct
      181 gaaacggtcg gttccgctgc gcgtcacgac gttgacctgt ccgccgccgc ttctgccgga
      241 ttcagccggg tagagcgagc gcaaaacgcg gaattcaccg attgagtcaa cgctcggata
      301 agcctgaata gtaatgtttg acccgcggtc ggtaatgtcc gcgccgtcga ccgtgaacgt
      361 attctggctg ctgcgcgcgc cgttgaccga aagattgatg gtgtttgcct gcccttcggg
      421 attggtcgtt ccgacataaa cctggtcggc aaggtcgctg gagacgcccg gcgcgagcgc
      481 gacgagctgg acgaagttac gattgttgag cgaaagctcg cgcacctggt cgccgttgat
      541 gaccgtgccg gcggtcggcg tcgtcagatc gaccgaaacg gcatccgatt cgaccgttac
      601 ggtttcctcg atgcgcccgg cttgcaaaac gatgtcaagc tgccggcgct gaccgacatc
      661 aatcttaacg tcggtgttaa ctgatttttt aaaattttgt gcctcgaccg ttaccgtgaa
      721 aacgctggtg gtcaggttcg gagcggaaaa ttcgccgtcg tcgctggtcg taatggtacg
      781 cacgacgatg ttatcctttt gtgcatccgt gatggtgacg gtcgctcccg gcacagccgc
      841 tcccgtatta tcacgaaccg ttccgacaat cgagccggtg acttcctgcc cgaaagcgaa
      901 cgcgctcata aacaaactta aagctaaagc taaaaacgcc aagcgagccg tttgtttgaa
      961 attatgtttc atattctttc tattccttct tgagcaaatt tgctgattct ttaatgacaa
     1021 acgataagtt ttatttaaat ttttggaaaa accttcaaat tttcgttatg gatgggcgcg
     1081 aaaattggtc tgaccaaaat atagatttcc gattttaatt ggatattgcg aaaatgttac
     1141 gacttaagaa attttataac cctaaatccg ttagttgtaa agcaaaaatg atgcccgacg
     1201 ctttttaaat tcgtccagct gcgactgcca ggctttttcg atttcttcgg gcgcgtcgcc
     1261 gcgtttgacg cgctccagaa tttcggcgtt gaccagcagg cgggcgtacc tgtcgacctg
     1321 ccattcgccc gggtaaagac ggcgcagcgc gaccgcgatt tcgattcccg tcctgacgga
     1381 ttcaaacgct ttccggtcgg tgataatgac gttgacgccg ccgagatttt cgtctttgta
     1441 aaccgaagcg cccggcttaa aacgaaccgg cacgaagcgc acgcccggca aatttcgcct
     1501 gttcagatac aaagccagtt tttgcccgtc gagccacggc gcgccgagaa cctcaaacgg
     1561 cgtatcggtt ccgcgcccga ccgaaacgtt ggtgacttcc aaaagtccga cgcccggata
     1621 aagcgtcgcc tgcgtcaagc tgcgcatatt cggcgatgga ttcgtccaaa tctgtccgct
     1681 ctcgtcaaac cacatcgcgc gcgaccagtt ttccattttc acgacgcgca cgtccgcgcc
     1741 gattttactt tcggcgttga acatctgcgc cagctcgccg acggtcattc cgtgccggac
     1801 gacaatcgga tgcgtggcga taaaattgag cttatccgca tcggcgagcg cgccttcgac
     1861 cgtcgagccg tttatcggat tgacgcggtc gagcacaaaa accgtttttt tcgctttcga
     1921 cgcttcttcc agaacgcctt tgagcgtgga aatataagtg taaaaccgca cgccgacgtc
     1981 ctgtatatca aaaacaatcg cgtcgagatc ggcgaactgt tccggtttcg gcttgcgcgt
     2041 ctcgccgtag agcgaataaa tcggcaagcc ggttttttcg tcggtcgaat ccgagatttt
     2101 ttcctggtcg agctgcccgc gaatgccgtg ctcgggcgcg aaaagcgcga cgagacgaac
     2161 attcggagct tcttttaaaa catcaatcgt cggttttccc gaaagatttt tgcccgtgtg
     2221 attcgtcacc aaaccgattc gcaaattttc cagacgctta aatttttctt tttccagaac
     2281 gtcgatgccg ttcaaaaccg tcacgttttg cgccgctcgc tgattggcga ctgccgaccg
     2341 ctgaaccagt tctttaaacc tcggaatctg cgccgcgacc tgcgccgatt aatcgttttc
     2401 cgcctgacga atagcttcga ccggcgcgtc ttcaatcgcc gaagcgacga ccgtcgccac
     2461 gcgcgagcgc agcggcgtca catcgccttt cccgtccggg tgaacgcggt tggaaagaaa
     2521 gacgacgaaa gtctgcgaaa cgcggtcaat ccaaactgaa gttcccgtaa aacccgtgtg
     2581 accgaatgag ccgagcggaa aaaggtcgcc gcgattcgac gaaaaagaag tgttcatatc
     2641 ccagcccagc ccgcgcgtcg cgccgtcttc ggaaacaacg accggcgcgg tcattctggc
     2701 aacagtctgc gccgataaaa ttctttttcc gtccagcgtg ccctcgttca gaagcatctg
     2761 gcaatagcgc gccaaatcgt cggcggtcga aaaaagtccg gcgtgaccgg cgacgccgtt
     2821 catccgaaaa gcggtcgggt cgtgaacttt cccgcgcaaa atctgatttc cggttttctc
     2881 gtcgccttca aatttcgagc cgagatagtt ctgctgtcct tttatgtttt cagtcggtgc
     2941 ggtgcggagt aaatcttccc gtatattttt aactaattta ttgccttcga atattttatc
     3001 tctcacgctg taaaccgaat cgtttagaaa gattgtgtct ttcaacccta tccggctgaa
     3061 aatttccgaa tttgcaaagt tgttcaaagg cgcagggtcg atattgcttt tttgtgaaca
     3121 atcaaaataa tcctggcagc cgattctctc gataatctcg ccgagcacga taaaaccgat
     3181 gtccgaataa acaaatcgcg ttcccggcgc gcttcgcaac ttttccttgt aaagcgcctg
     3241 ccgcattcct tcgctcgttt cccatttttc ccgcaaatcg aaatccggcg cgtaaccgga
     3301 aacgtgcgtc aaaagctgct gaatcgttac gcgcctcgcg ttttcgtcct gaatttcggg
     3361 aatgaatctg ccgaccgtat cgtttaaacg aagctttccc tgctcgacga gaatcatcac
     3421 ggaagtcgcc gtcgcaaccg gtttggtcag cgacgcgaca tcgaaaatcg tgtcgatcgt
     3481 cattttctcg atcgtcggag tcaatgaacg attgccgtaa gctttgcgaa aaacgatttt
     3541 tcctttatgt ccgaccagaa cgaccgcgcc gggcagtttt ttatcggcga tgtctttggc
     3601 gactaaagcg tcaatttgat tgagctttgc cgcgttcatc ccgaccgtct gcggcaaagc
     3661 gaccggcagg ctctgtgctt tcgcaaaggc gaaaggcaaa aagcaaaagg caaaaaacat
     3721 cgccgccgaa acgcggaaac gcaaagaata attttgcagt ttatctatcg aaaacaaacc
     3781 gaccaccgac gatttacgac tgacaaattt cttcattttt tcgatttcag ttccttttct
     3841 ttttccgcga agctttcgcg gcagaggcgg gcaaattcgt gcgcaatcgg cgaaaaataa
     3901 ccgccgcgca gacgcgccaa gcccaattcc cagttcatcg tcgcgccttc aatccaccag
     3961 gcgacgaatt tgccttcggc gaccgactgc ttgacggttt tcgcgctggc gatgccgacg
     4021 cccatattat tttcgaccat tcgatttatc gccgactggc gcgacaattc catcacgacg
     4081 ttcggcttga cgccgacctg caaaaagaaa tcgtcaatct gccgccgcgt gttgccgccc
     4141 tgctcgccca gtatgagcgg ctcgtcggcg agcgccgcgg cgctgatgac tttttgtttg
     4201 gcgagcgggt gtttcgggtt ggcgatggcg acaatctcgt cgctgaataa aagctcggtt
     4261 tgaatgttcg agttttcgac cggcagcgag acaaaggcga cgtcgctttc gccgttcata
     4321 attttctgca ccagcgccgc gctcgttccc gaagcgaccg agacgaccgc gttcggaaac
     4381 ttcgctttta aattttgcag aatcttcggc aattggttgg tcgtaaacat cgccgacgcc
     4441 gagccgatgc gcaggcgacc gtattccgcg cccgcgattt cggcgatttc ctcgaccgcc
     4501 gcgtcgtgct cgcgcagaat ttttctggct ctttcgagca gcaaatcgcc cgcttcggtc
     4561 attagaacgc ggcgcggcgt gcgcgtaaac agcggcaagc cgacttcatc ttcgagctgc
     4621 cgaatctgca tcgaaattgc cgcctgcgtc acgtggacgc gcttggcgcc cgccgtaaaa
     4681 gtttttgctt ccgcgattgc cacgaaagct ctcaattgtc ggatttccat aaatgattgg
     4741 tcattcgcca tttatccttt gtgctccggc gaatcgggcg ttactcaaac ccgataaaga
     4801 taaacctcgc ccgagcgcaa aggacgtacg aatggagacg aaataaaaag acaaatatca
     4861 aaaagcctta t
//
LOCUS       DASWNE010000955         2747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_343013, whole genome shotgun sequence.
ACCESSION   DASWNE010000955 DASWNE010000000
VERSION     DASWNE010000955.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343013"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VGB68_18615"
     CDS             complement(<1..375)
                     /locus_tag="VGB68_18615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051316.1"
                     /translation="MKKNPILIFSVIALFSLTAHVFAQSKKAVPPIRSAPAAPAETSR
                     DNSRTNFSGFFDGSTYKNKFFGLKITVPESWMIQGNATNREIKQLGADSVKGKNEQTE
                     KALDAAIDRLTVLFTASKDILGM"
     gene            complement(409..1107)
                     /locus_tag="VGB68_18620"
     CDS             complement(409..1107)
                     /locus_tag="VGB68_18620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051317.1"
                     /translation="MNYFNYFSEIEETFNRRRGKYLFLSPLDWALIESWQERGVPLHI
                     ILRGIEKVFDGVDNQPKRRRSVKSLMYCREEIEAQYEEWLERQVGKSQTDGDGAAETE
                     IETSLFSDENVKTHLENAAQSLTRAQAKAKGELREVLLRVSARLAELEKSFNDAENLE
                     SALGDLEHLIDAALLETADEKTLASLKAETDKLMAQYRGKMEKEVYDRTFDLMLLKTL
                     REAAEIPRLSLFYL"
     gene            complement(1217..1477)
                     /locus_tag="VGB68_18625"
     CDS             complement(1217..1477)
                     /locus_tag="VGB68_18625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051318.1"
                     /translation="MELDENAKRAVRELGDAINSAIEKSERVIEAIERLREIGYEPNL
                     SLKLEIGLLEIQRDAEDAARDVSLKLTDEDLRTLRSMKIRLE"
     gene            complement(1708..2373)
                     /locus_tag="VGB68_18630"
     CDS             complement(1708..2373)
                     /locus_tag="VGB68_18630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051319.1"
                     /translation="MKDSQRRKLDKFEREEVFLNDNIADFPPASPGGKALAELSAVIS
                     QIRAFAAEQVSGAGSSRQHIGVKDEEVDALMRLIRNINRAANAFEDEIPGSDLKFRLP
                     RNRSEQNILATARAFLADAEPLKAKFIEYGLAADFTDKLQALIEAVDERAAAADSAGE
                     QKAGATGGLIDIVRKGMSISRRLDSIVRIKYEDNAAKLSAWTTASHLKKAPEKTQDEP
                     PPV"
     gene            complement(2390..2617)
                     /locus_tag="VGB68_18635"
     CDS             complement(2390..2617)
                     /locus_tag="VGB68_18635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051320.1"
                     /translation="MNDAHSQLTGRHSHVTGEDSQLTDEDSHVNDEDSQVNDEDSHVN
                     DEHSQVNDEHSHVTDTDSQVNGGDSQLKTTN"
BASE COUNT          583 a          728 c          683 g          753 t
ORIGIN      
        1 cattccgaga atatctttgg aagcggtaaa cagcacggtc aaacggtcga tagccgcgtc
       61 cagagccttt tcggtctgct cgtttttacc ctttaccgag tcggcgccga gttgttttat
      121 ctctcggttg gtggcgtttc cctgaatcat ccagctttcg ggaacggtta ttttcaagcc
      181 gaaaaactta tttttatatg tgctgccgtc aaagaagccg gaaaaatttg tccgggaatt
      241 gtcccggctc gtttcggcgg gcgcggcggg cgcgcttcgg attggcggaa ccgccttttt
      301 gctctgcgcg aaaacgtgag cggtcagcga aaataaagct atgacggaga aaattaatat
      361 cggatttttt ttcatatttt tcgttctcta tttcatattt ttcgttctct ataaataaaa
      421 caggctcagg cgcggaattt ccgcggcttc gcgcaaggtt ttgagcagca tcaaatcgaa
      481 agtccggtcg taaacttctt tttccatttt gccgcgatac tgcgccatca gtttatcggt
      541 ttcggctttg agcgaggcga gagttttttc gtccgccgtt tcgagcagag cggcgtcgat
      601 gagatgttcc aaatcgccga gcgcgctttc cagattttcc gcgtcgttaa agcttttttc
      661 aagctcggcg agccgcgccg aaactctcag cagcacttcg cgcaattcgc ctttggcttt
      721 cgcctgcgcg cgcgttaagc tctgcgcggc attttccagg tgcgttttga cattttcgtc
      781 cgaaaaaagc gaggtttcaa tttcggtttc cgccgcgccg tcgccgtcgg tctgactttt
      841 cccgacctga cgctccagcc attcttcgta ttgcgcttcg atttcctcgc ggcaatacat
      901 cagcgatttc acgctccggc ggcgtttcgg ctggttgtcc acgccgtcga aaactttctc
      961 gatgccgcgc aggataatgt gcagcggcac gccgcgctcc tgccacgatt caatcaaagc
     1021 ccagtcgagc gggcttaaaa acaggtattt cccgcgacgg cggttaaagg tttcttcgat
     1081 ttcggagaag tagttgaagt aattcaaggg attttagagt cggcaaacgg tcttcggtct
     1141 tcgttaaaga aactgctcga tatcgagaag aagaaaaatc gaagaccgaa gaccgaaaac
     1201 caaagaccgg agcccgttat tccagacgga ttttcatact gcgcagcgtg cgcaaatcct
     1261 cgtcggttaa tttcagagac acgtcgcgcg cggcgtcttc ggcgtcgcgc tggatttcca
     1321 gcaagccgat ttccagcttg agcgataaat tcggctcgta accgatttcg cgcaggcgct
     1381 cgatggcttc gataacgcgc tcggattttt cgattgccga atttatcgcg tcgcccagct
     1441 cgcgcaccgc ccgttttgca ttttcgtcaa gttccattgc cgttagtaag aaaatgctac
     1501 gtcagataaa tgcaaaacgc aaaatgcaaa gtgcaaaatg aagaagccgg aagacggaag
     1561 acggaagtca aaagacagaa gtcagaagtc agaagtcagt agttagaaga caaatttatg
     1621 tcgtcttccg tcttccggct gctgacttct atcttctgac ttccgtctgc ttattcatca
     1681 atcatcgttt cgccttcatc gttttcttta cacgggcggc ggctcgtctt gcgttttctc
     1741 cggcgctttt ttgagatggc tggcggtcgt ccacgcggag agcttggcgg cattatcttc
     1801 gtatttgatg cggacgatgg aatccagacg gcgcgagatg ctcattccct tgcgaacaat
     1861 gtcgatcagc ccgccggtcg cgcccgcctt ttgctcgccc gcgctgtcgg ctgccgccgc
     1921 tcgctcgtcc accgcttcga tcaacgcctg taatttatcg gtaaaatcgg ctgccagacc
     1981 gtattcgata aacttcgctt tcagcggctc ggcgtccgcc agaaacgccc gcgccgtcgc
     2041 cagaatattc tgctccgagc ggttgcgcgg caggcgaaac ttcaaatcgc tgcccggtat
     2101 ttcatcctcg aaggcgttgg cggcgcgatt gatattgcga atcaaccgca tcagcgcgtc
     2161 aacctcttca tccttgacgc cgatgtgctg gcgcgagctg cccgcgcccg aaacctgctc
     2221 ggcggcaaac gcccttatct gtgaaataac cgccgacagc tccgccagag ccttcccgcc
     2281 gggcgacgcc ggcggaaaat cggcaatatt gtcattcaaa aacacttctt cgcgctcaaa
     2341 cttatcgagt ttgcgcctct gactgtcttt catgggctta agtctccttt taattggttg
     2401 tttttagttg tgaatctccg ccgttcacct gtgaatccgt gtcggtcacg tgtgaatgtt
     2461 catcgttcac ctgtgaatgt tcatcgttca cgtgtgaatc ttcgtcgttc acttgtgaat
     2521 cttcatcgtt cacgtgtgaa tcttcatcgg tcagttgtga atcttcgccg gtcacgtgtg
     2581 aatgtctgcc ggtcagttgt gaatgtgcat cgttcacctg tgaatgctcg aaattcaccg
     2641 aaaatttaca acaagtcgcc gcttttggca agtaaaaaat aattttcaat gataaataaa
     2701 agaatccata aaaatgaagg ctttattttt gtcgtttgat gctctag
//
LOCUS       DASWNE010000956         5457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_243399, whole genome shotgun sequence.
ACCESSION   DASWNE010000956 DASWNE010000000
VERSION     DASWNE010000956.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5457
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243399"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VGB68_18640"
     CDS             <1..534
                     /locus_tag="VGB68_18640"
                     /inference="COORDINATES: protein motif:HMM:NF016952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016603 - glutaminyl-peptide
                     cyclotransferase activity [Evidence IEA];
                     GO_process: GO:0017186 - peptidyl-pyroglutamic acid
                     biosynthetic process, using glutaminyl-peptide
                     cyclotransferase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HEY0051321.1"
                     /translation="IYQITWRERTAFVYDVKDLNLLKEIRYQGDGWGLTHDATNLIMS
                     DGTHIIRFVNPENFQTVRTVAVFRENGQPLLNLNELEYVKGEIWANIWHSENNATDSE
                     HGFLPNIGKPNYIARIDPASGKVVGWIDLAGISPEDVKNSENTLNGIAYDAQNDRIFV
                     TGKRWKNLFEIKLKPKP"
     gene            558..1034
                     /locus_tag="VGB68_18645"
     CDS             558..1034
                     /locus_tag="VGB68_18645"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEY0051322.1"
                     /translation="MTEQDEYFMRRAIALAQNGIDSNSGGPFGAIVVRAGEIIGEGCN
                     QVTSTNDPTAHAEVVAIRAACAHLGSFQLDECVIYTSCEPCPMCLGAIYWARPARVFF
                     GCTREDAARIGFDDQFIYEEIEKNHEHRQMKLANFLRDEALVVFQNWENKADKKEY"
     gene            1117..1575
                     /locus_tag="VGB68_18650"
     CDS             1117..1575
                     /locus_tag="VGB68_18650"
                     /inference="COORDINATES: protein motif:HMM:NF016535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY0051323.1"
                     /translation="MSSFFNNYFFIVLAVAAGVMMPTQAAINNKLADAVESPILSAFI
                     SFGVGTLALLLYILATGVPLTNLASAKNAPLIAWTGGLLGAFFVAATVTVVSKLEVAL
                     TFSLIIAGQMLITLVIDHFGFLGVPVKEINIPRLLGVTLITIGVILIRRF"
     gene            1702..3090
                     /locus_tag="VGB68_18655"
     CDS             1702..3090
                     /locus_tag="VGB68_18655"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEY0051324.1"
                     /translation="MIKFILNHKSVETDLPAGTTVLDFVRYRQNLKGTKIGCREGDCG
                     ACTILVGELSGDAVKYRSMTSCLMPLANAAGKHIVTVEGINKPDGELTGVQQALVDES
                     GTQCGFCTVGFVMSLTGFCIDETPKTCESAISSIDGNICRCTGYKSIERAAENLTRAL
                     NEKNGKSLLDFAVEKEFVPDYFSGIKNRLSQLNQKTRNSELKTQNSFFVGGGTDLYVQ
                     RHDEMVYAAAENLFDNSALRGIVENGDRVEIGASATVTDLLVSPVMQRIFPNLYKHLK
                     LVSSTPIRNMATLAGNFINASPIGDMTAWFLGLDASVVFNDEREIPLRELYLGYKTLA
                     KTENEFITKIRFRRNFSHFNFEKVSKRTFLDIASVNTAISLKTADGKITEAHASAGGV
                     APIPLYLRKTSEFLRGGEINEATISQANEILQTEISPISDVRGSADYKRLLLRQLFTA
                     HFVELFGYQPSI"
     gene            3111..5390
                     /locus_tag="VGB68_18660"
     CDS             3111..5390
                     /locus_tag="VGB68_18660"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY0051325.1"
                     /translation="MKNVDSKSHVRGESIYLDDIPLIQGTLYACVYDSPVAHGVLKSV
                     EIAEAEKSEGVVKIILAKDLIGENQIGAVLPDEPLLADAEVHFQGMPVAIVLAETEEQ
                     ARIAAKKIKAEIEPLEVVTDPRAAWAKGDLIVPPKHFKLGDAGKAFKTSEHVFSGRAD
                     VNGQEHLYIETQGAYTVPTEQGGMRVYSSTQGPTAVQRCVSRVTALPMNLVEVDVARL
                     GGGFGGKEDQANAWAAICAVGTQLTKRPVKYALHRMEDMRMTGKRHPYSADYKIGLDK
                     DLKIVAYEAVFYQNAGASADLSPPVLERTLFHCTNAYFVPNVTATAYSCRTNLPPNTA
                     FRGFGGPQGMYVIESAIAHAAEKLNVSASEIQRKNLLRDGDEFPYGQKAESEAATSWT
                     QADAEFDFARLQKQTDEFNEQNKFVKKGLALMPICFGISFTKTPMNQARSLVHVYTDG
                     SVAVSTGAVEMGQGVNTKIAQVASTVFSMPITRVRVHTTNTLRIANTSPTAASAAADL
                     NGKATQIACETILKRLKDVAKDLVEAQSAEDISIENGFVFRKGESTNLDWLVLVNEAH
                     MQRVNLSEHAHYATAGINFDWSIAKGHPFAYHVYGTAAVGVTVDCLRGIYEVDFVKAC
                     HDFGSSMNTSVDYGQIEGGIVQGIGWMTMEEIFYDAEGKLRSNALSTYKIPDVYSAPK
                     QIDILPLETSRENLAIFNSKAVGEPPLMYGIGAYFAIRNAVKAFNTENLPAFDAPLTP
                     EKVLMNLYRDATKKMKAGK"
BASE COUNT         1354 a         1382 c         1482 g         1239 t
ORIGIN      
        1 atctatcaaa taacgtggcg cgaacgaacg gctttcgttt atgacgtgaa agacttaaat
       61 ttgctcaaag aaattcgtta tcagggcgac ggctggggtt tgacgcatga cgcgacgaat
      121 ctgattatga gcgacggcac gcatatcatt cgcttcgtca acccggaaaa tttccaaacc
      181 gtccgcaccg ttgcggtttt ccgcgaaaac ggacagccgc ttcttaacct taacgaactc
      241 gaatatgtca aaggcgaaat ctgggcgaac atctggcatt cggaaaacaa cgcgaccgac
      301 agcgagcacg gctttctgcc gaatatcggc aagccgaatt acatcgcccg catcgacccg
      361 gcttccggca aagtcgtcgg ctggattgat ttggcaggca tctcgcccga agatgtaaag
      421 aacagcgaaa atactttgaa cggcatcgct tacgacgcgc agaacgaccg catttttgtc
      481 acgggcaaaa ggtggaaaaa tctttttgag ataaaattaa aaccgaaacc gtaagcggca
      541 gcgatttgaa tcggaaaatg acggaacaag acgaatattt tatgcgccgc gcgattgctc
      601 tggcgcaaaa cggaatcgac tcgaactcgg gcggaccttt cggcgcgatt gtcgtgcgcg
      661 cgggcgaaat tatcggcgaa ggctgcaacc aggtgacttc gaccaacgac ccgacggcgc
      721 acgccgaagt cgtcgccatt cgtgccgcct gcgcgcatct cggctctttc cagctggacg
      781 aatgcgttat ttacacttcc tgcgagccgt gcccgatgtg tctgggcgcg atttactggg
      841 cgcgccccgc aagagttttt ttcggctgca cgcgcgagga cgccgctcga atcggttttg
      901 acgaccagtt tatctacgag gaaatcgaaa aaaaccacga gcacaggcaa atgaagctcg
      961 ccaatttttt gcgcgacgaa gcgctcgtcg tctttcaaaa ctgggaaaat aaagcggata
     1021 aaaaggaata ttaaaccgta agcggtaagt ggtaaacggt aagtgtaaat tttttcatat
     1081 cacttgccgt ttaccactta ccgcttacca ctaaaaatga gttctttttt caacaattat
     1141 tttttcatcg ttctcgccgt cgccgccgga gtgatgatgc cgacgcaagc cgccattaac
     1201 aacaagctgg ctgacgcggt cgaaagcccg attctatcgg cttttatctc atttggcgtc
     1261 ggcacgctcg ccctgcttct gtatattctg gcgaccggcg tgcctttaac gaatctggct
     1321 tcggcgaaaa acgcgccgct tatcgcgtgg acgggcggac tgctcggcgc gtttttcgtg
     1381 gcggcgaccg tgacggttgt ttcaaagctc gaagtggcgc tgacatttag cttaatcatc
     1441 gccggacaga tgcttataac tctggtcatc gaccatttcg gctttctcgg cgttccggtc
     1501 aaggaaatta atattccaag gcttttgggc gtcacgctga ttacgatcgg cgtgattttg
     1561 attcgtcgtt tttagtttac aataagaaaa ttaaccgagg attaagggga taaacaggga
     1621 ttaaaagaaa agattattaa tgattttatt ttatctcctt catcctcttt atcctcggtt
     1681 aattcttctt cttaattttg aatgattaaa tttattctca atcacaaaag cgtcgaaacg
     1741 gatttgcccg cgggcacgac cgtgctcgat tttgtccgct atcgtcaaaa tctgaagggc
     1801 acgaaaatcg gctgtcgcga aggcgactgc ggcgcgtgca cgattctggt aggcgagctg
     1861 agcggcgacg cggtgaaata tcgctcgatg acttcgtgcc tgatgccgct ggcaaatgcc
     1921 gccggcaaac atatcgtgac ggtcgaagga atcaataaac cggacggcga attaaccgga
     1981 gttcaacagg cgctggtcga tgaaagcgga acgcagtgcg gtttttgcac ggtcggtttc
     2041 gtgatgtcgc tgaccggatt ctgtatcgac gaaacgccga aaacctgcga gtcggcaatt
     2101 tcgtcgatcg acggaaacat ctgccgctgc accggctata aatcaatcga gcgcgcggcg
     2161 gaaaatctga cgcgcgcttt gaatgagaaa aacggaaaat cgctgctcga ttttgcggtc
     2221 gaaaaagaat ttgttcccga ttatttttcc ggcatcaaaa atcggctttc gcaattaaat
     2281 caaaaaactc ggaactcgga actcaaaact cagaactctt ttttcgtcgg cggcggaacg
     2341 gatttatacg tccagcgcca cgacgagatg gtttacgctg cggcggaaaa tcttttcgat
     2401 aattcggctc tgcgcggaat cgtcgaaaac ggcgaccggg tggaaatcgg cgcttccgcc
     2461 acggtcacgg atttgctcgt ttcgcccgtg atgcagagga tttttccgaa tctttacaaa
     2521 cacctgaagc tcgtttcctc gacgccgatt cgcaatatgg cgacgctcgc gggaaatttc
     2581 atcaacgctt cgccgattgg cgatatgacc gcctggtttc ttggcttgga tgcgagcgtc
     2641 gttttcaatg acgaacgcga aattcccctg cgcgagcttt atctcggcta caaaacgctg
     2701 gcgaaaacgg aaaacgaatt tatcacgaaa attcgttttc gccgaaactt ttcgcatttt
     2761 aatttcgaga aagtcagcaa gcgcacgttt ttagacatcg cttcggtcaa cacggcgatt
     2821 tcactgaaaa cggcagacgg caaaatcacc gaagcgcacg cgtcggcggg cggcgtcgcg
     2881 ccgattcctc tgtatctgcg aaagacttcc gaatttttgc gcggcggtga aattaacgag
     2941 gcgaccattt cacaggcaaa cgaaattctg caaacggaaa tcagcccgat ttccgacgtg
     3001 cgcggctcgg cggattataa aagactgctt ctgcgtcagc tttttacggc gcatttcgtg
     3061 gaattgtttg gctatcaacc gagtatttag ggtttgattt atagaacacg atgaaaaacg
     3121 tagattcaaa atcgcacgtg cgcggcgaat caatttatct ggacgacatt cctttgattc
     3181 agggaacgct ctatgcgtgc gtttacgatt cgcccgtcgc gcacggcgtt ttgaaatcgg
     3241 tcgaaatcgc cgaagccgaa aaatcggaag gcgtcgtgaa aatcattctg gcgaaagatt
     3301 tgatcggcga aaatcaaatc ggcgcggttt tgccggacga gcctctgctg gcggacgcgg
     3361 aagttcattt tcagggaatg ccggtggcga ttgtgctggc ggagacggaa gaacaggcgc
     3421 gaatcgccgc gaaaaaaatc aaagcggaaa tcgagccgct cgaagtcgtc accgacccgc
     3481 gcgcggcgtg ggcgaaaggc gatttaatcg tgccgccgaa acattttaaa ctgggcgacg
     3541 cgggcaaagc ttttaaaacc agcgagcacg ttttttcggg gcgcgccgac gtgaacgggc
     3601 aggaacatct ttatatcgaa acgcagggcg catacaccgt tccgaccgag cagggcggaa
     3661 tgcgcgtcta ttcttcgacg cagggtccga cggcggtgca gcgctgcgtc tcgcgcgtga
     3721 cggcgctgcc gatgaatctg gtcgaggtcg atgtcgcgcg gctcggcggc ggcttcggcg
     3781 gcaaggaaga ccaggcgaac gcgtgggcgg cgatttgcgc ggtcggcacg cagttgacga
     3841 agcgtccggt caaatacgcg ctgcaccgga tggaagatat gcggatgacc ggaaagcgcc
     3901 acccgtattc ggcggattat aaaatcggtc tggacaagga tttgaaaatc gtcgcctacg
     3961 aagccgtgtt ttatcaaaac gcgggcgcgt cggctgattt gtcgccgccg gttctggagc
     4021 gaactttgtt tcactgcacg aacgcttatt tcgttccgaa cgtgacggcg accgcttaca
     4081 gctgccgcac gaatctgccg ccgaacacgg cttttcgcgg cttcggcgga ccgcaaggaa
     4141 tgtacgtcat cgaatcggcg atcgcgcacg cggcggaaaa gctgaacgtt tccgcttcgg
     4201 aaatccagcg aaaaaatctt ttgcgggacg gcgacgaatt tccttacggt caaaaagccg
     4261 aaagcgaagc cgccacgagt tggacgcaag ccgacgctga attcgatttt gcgcggctgc
     4321 aaaaacagac ggacgaattt aacgaacaaa acaaattcgt caaaaaaggc ttggcgctga
     4381 tgccaatctg cttcggcatc tcgtttacca aaacgccgat gaatcaggct cgctcgctcg
     4441 tccacgttta cacggacggc tcggtggcgg tttcgaccgg cgcggtcgag atgggacagg
     4501 gcgtgaacac gaaaatcgcg caggtcgctt cgaccgtttt ttccatgccg ataacccgcg
     4561 tccgcgttca cacgacgaat actctgcgca tcgccaatac ttcgccgacg gcggcttcgg
     4621 cggcggcgga tttgaacggc aaagcgacgc agattgcctg cgagacgatt ttaaaacgcc
     4681 tgaaagacgt ggcgaaggat ttggtcgaag cgcagagcgc cgaagatatt tccatcgaaa
     4741 acggtttcgt tttccgcaaa ggcgaaagca cgaatctgga ctggctcgtg ctcgtcaacg
     4801 aagctcatat gcagcgcgta aacttaagcg agcacgcgca ttacgccacc gccggaatca
     4861 atttcgactg gtcgatcgcc aaaggtcatc cgttcgccta ccacgtctac ggcacggcgg
     4921 cggtcggcgt gacggtcgat tgcctgcgcg gaatttacga agtcgatttc gtcaaggctt
     4981 gtcacgattt cggcagctcg atgaatactt cggtcgatta cgggcaaatc gaaggcggaa
     5041 tcgtgcaggg catcggctgg atgacgatgg aagaaatttt ttacgacgcg gaaggcaagc
     5101 tgcgctcaaa cgcgctttcg acctacaaaa ttcccgacgt ttattccgcg ccgaaacaaa
     5161 ttgacattct gccgcttgag acgagccgcg aaaatctggc gattttcaat tcgaaagcgg
     5221 tcggcgagcc gccgcttatg tacggcatcg gcgcgtattt cgcgattcgc aacgcggtca
     5281 aagcgtttaa tacggaaaat ttgcccgcct ttgacgcgcc tttaacgccc gaaaaagttt
     5341 tgatgaattt gtatcgcgac gcgacaaaga aaatgaaagc cggaaaataa aattcgcgcc
     5401 ggttagcggt taattttctt ttcaaatgaa aaaagaatgg gaaatctggc aattcgt
//
LOCUS       DASWNE010000957         3714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_136155, whole genome shotgun sequence.
ACCESSION   DASWNE010000957 DASWNE010000000
VERSION     DASWNE010000957.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136155"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VGB68_18665"
     CDS             complement(<1..654)
                     /locus_tag="VGB68_18665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY0051326.1"
                     /translation="MAQISLDKFRNIGIMAHIDAGKTTTTERVLFYTGVSHKIGEVHE
                     GTATMDWMEQEQERGITITSAATTCFWKRNNIEHRINIIDTPGHVDFTMEVERSLRVL
                     DGAVAVFDGVAGVEPQSETVWRQADKYGVPRICFINKLDRAGASFERSFDSILKRLGA
                     NAVALQIPIGLEDQLKGVVDLITMKGLIWNDETKGAEYEVTDIPADLVDAATEAREKL
                     "
     gene            complement(740..1210)
                     /gene="rpsG"
                     /locus_tag="VGB68_18670"
     CDS             complement(740..1210)
                     /gene="rpsG"
                     /locus_tag="VGB68_18670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEY0051327.1"
                     /translation="MPRRRVAGKREVLPDPIYNSVMVSKFINGVMWQGKKSVAEQIFY
                     GAMQKLADRTGDEPLKVFKKALDNVSPTLEVKSRRIGGATYQVPLEVSKDRRNTLAIR
                     WIVSNARNRNEKTMQDRLVGELLDAINNRGGAVKKREDVHRMAEANKAFAHYRF"
     gene            complement(1306..1710)
                     /gene="rpsL"
                     /locus_tag="VGB68_18675"
     CDS             complement(1306..1710)
                     /gene="rpsL"
                     /locus_tag="VGB68_18675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEY0051328.1"
                     /translation="MPTINQLVRKGRQAVKYKTASPALQSSPQKRGVCTRVYTQTPKK
                     PNSALRKVARVRLTNGIEVTTYIPGIGHNLQEHSIVLIRGGRVKDLPGVRYHIIRGTL
                     DSSGVANRNQARSKYGAKRPKAGAAAAGAKKK"
     gene            complement(2236..2886)
                     /locus_tag="VGB68_18680"
     CDS             complement(2236..2886)
                     /locus_tag="VGB68_18680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051329.1"
                     /translation="MTFVGNTFSQNNNGNCVKCVGAGHGWICQAAPGGGDACTTDGYN
                     CTLINPCPSFGGANCPPNAQDPTKPKIILNISDELVREVGKSNPRLGVALIGLRNLPP
                     LSYSKGMINTAPVELSQNDIEEQLAATASSKDYVAQLRVKTGEAFKKGLLPTVYNFSI
                     EPSSAPDAYILKLQTAEVGSFGSLVSIDINLSEMKEATRDAKEPTRALKAGSWKID"
     gene            complement(3134..3505)
                     /locus_tag="VGB68_18685"
     CDS             complement(3134..3505)
                     /locus_tag="VGB68_18685"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY0051330.1"
                     /translation="MDRRILFLKQEVASNLQKQYSVEKLALSVNLSASHLQKLFKAET
                     GMSAVQYVRHLKMEKARELLEGSFLLIKEIRKEVGAPDLSHFVRDFKVSYGLTPSEYR
                     KQHWQKLEIENRDSKTDSIRH"
BASE COUNT          863 a          904 c          850 g         1097 t
ORIGIN      
        1 aagtttttcg cgcgcttcgg tcgcggcatc gaccaagtca gccggaatgt ccgtgacctc
       61 gtattccgcg cctttggttt cgtcgttcca aatcaagcct ttcatcgtga taaggtcgac
      121 gacgcctttc agctggtctt ccagaccgat cgggatttgc agagcgacgg cattagcgcc
      181 gaggcgcttt aaaatcgaat cgaacgagcg ttcaaacgaa gcgccggcgc ggtctaattt
      241 attgataaag cagatgcgcg gaacgccgta tttgtcggct tgacgccaga cggtttccga
      301 ttgcggctcg acgccggcga cgccgtcgaa aacggcgacc gcgccgtcca gaacgcgcag
      361 agagcgctcg acttccatcg tgaaatcgac gtgtcccggc gtatcgataa tattgattcg
      421 gtgttcgatg ttgttgcgct tccagaaaca ggtcgtcgcc gcggaagtaa tcgtgatgcc
      481 gcgctcttgt tcctgttcca tccagtccat cgtcgccgtt ccttcgtgaa cttcgccgat
      541 tttgtgcgaa acgcccgtat agaacagcac gcgctcggtc gtcgtggttt tacccgcgtc
      601 gatgtgcgcc atgatgccga tgtttctaaa tttgtctaag ctaatctgtg ccatttcatt
      661 aagtggtaaa cggtaagcgg taagtggtaa gtgaaagcaa cgaacttatc acttaccgtt
      721 taccactttc cactattcat taaaatctat aatgtgcgaa cgctttgttc gcttccgcca
      781 tacggtgaac gtcttcgcgt tttttcaccg cgccgccgcg attgttgatt gcgtcgagca
      841 gctcgccgac cagtctgtcc tgcatcgttt tttcgttacg attgcgcgcg ttcgagacaa
      901 tccagcgaat tgccagcgtg ttgcggcggt ctttcgaaac ttcgagcgga acctgatacg
      961 tcgcgccgcc gatgcgccgg gatttgactt ccagagtcgg cgagacgtta tccagtgctt
     1021 ttttgaaaac tttcaaaggc tcgtcgccgg ttctgtcggc gagtttttgc atcgcgccgt
     1081 aaaaaatctg ttcggcgact gattttttgc cctgccacat cacgccgttg atgaatttcg
     1141 agaccatcac gctgttgtaa atcggatcgg gcaaaacttc tctttttcct gcaactcttc
     1201 ttcttggcat aactttattc aattactaat tacgaatttc aaattacgag atacgagcga
     1261 ttgtattttt ctttcaattc gtaattcgta attgttaatt cgtaattact ttttcttcgc
     1321 tcctgctgcg gcagcaccgg ctttcggtcg tttcgcgccg tatttcgagc gcgcctgatt
     1381 gcgattcgcc acaccgctcg aatcgagcgt tccgcggata atgtgataac gaacacccgg
     1441 caagtctttg acgcgaccgc cgcgaatcaa aacgattgag tgctcctgca agttgtgccc
     1501 gatgcccgga atataagtcg tgacttcgat tccgttggtc aggcgaacgc gggcgacttt
     1561 gcgcagcgcc gagttcggtt ttttcggagt ttgcgtgtaa acgcgcgtgc aaacgccgcg
     1621 tttctgcggg ctcgattgca gcgccggaga cgccgttttg tatttcaccg cctggcgacc
     1681 tttgcgaaca agttggttaa tcgttggcat tctttttaaa ctctcttcaa aataaaagcg
     1741 aaagtaaccc ctaaacctgc ctcttgccga aactcgttcg gcagtcagcg caagcggcta
     1801 cttgcgactc aattaatgat taaaccttag ggaacgtctt ttttatttta tctgctgcgt
     1861 agattctgac ttccctggaa cttctcaagt cattaacttt tctgtggcgt ggctttattt
     1921 taataaaggt aagcaaactt ggttggtttg ccctgaattt ttaaaactca gttttacgcc
     1981 acaataactg ttcgcgtttg aaaccaaacg ctctacgcat tcaaaacaga ccgtattgct
     2041 actgtccatc tcagacgaaa tgtccgaaac ttcagattct acgaaaccgg ctatccaatg
     2101 tcaagtgaac ctttgcacaa aagcgaattt tcccgttttt gccgattact gtaaaaattg
     2161 ctatgaaaaa tgaaaaggtc gccctttgat ttccggcgac cttctttaat acgctttctt
     2221 aaaaattctt taaatttaat ctattttcca cgacccagcc ttgagcgctc tggtcggttc
     2281 ttttgcgtcg cgcgtcgcct ctttcatttc ggacaagtta atgtcgattg aaaccagact
     2341 gccgaacgag ccgacttcag cggtttgaag ctttaaaata taggcatccg gcgcggagga
     2401 aggctcgata gaaaaattat aaaccgtcgg gagtaatcct tttttaaaag cctcgccggt
     2461 tttgactctt aactgggcga cgtaatcttt ggagcttgcc gtcgcggcga gttgttcttc
     2521 gatatcgttt tggctcagct cgaccggcgc ggtgttaatc attcctttgc tgtagctgag
     2581 cggcggtaaa ttccttaagc cgataagagc gacgccgagt cgcggattgg attttcctac
     2641 ttctctgaca agctcatctg aaatattaag aataattttc ggttttgtcg ggtcctgcgc
     2701 gttcggcgga caattagcgc cgccgaatga aggacacggg ttaatgagcg tgcaattata
     2761 gccgtccgtc gtacaggcgt cgccgccgcc cggtgcagcc tgacaaatcc acccgtgacc
     2821 cgccccgacg catttaacgc aattgccatt gttgttttgt gaaaatgtat ttccgacaaa
     2881 agtcatacat aacactgaga atacgaccag cgaaaaaaaa gttcttctaa atttcttcat
     2941 catttcctcc attttaaaga tgcataatta aggctcaaaa caatcgccgc atcaacagca
     3001 attgcagcct gtagagacta gagatctagt ttagagagac taatttcctg agtcgtggga
     3061 gcaacccgtt gttttaatct tgaattttga gagattgagt agtcaagctt tttcattttg
     3121 ccaaaactgt tatttagtgg cgaatactat ctgttttcga gtcccggttt tcgatttcca
     3181 gtttttgcca gtgctgtttg cggtattcgg aaggcgttaa accatagctg actttaaaat
     3241 cccggacaaa atggctcaag tccggcgctc cgacttcttt tctgatttcc ttgataagca
     3301 ggaaactgcc ctccagcaac tcgcgcgctt tttccatctt cagatgtcga acgtactgga
     3361 cggctgacat tccggtttcg gctttaaaca gtttttgcag atgcgaagcg gaaagattta
     3421 ccgacaaagc caatttttcg accgagtatt gtttttgtag atttgatgcc acctcttgct
     3481 tgagaaataa gattcttcta tccattgcgg gtctccttgt aaaatggatt agattatcaa
     3541 ccagcaaaca acaaccaatt ttgcgatttt cgaagtttat cacaactgca ttttgaggta
     3601 aataaaaatt gttgctatgc tttttgtttt aatttccgca attttatgag gttctatgtc
     3661 aacgaaattt gaggtttttt taaatcaatt tgacgaaaaa gcgtggctcg atgc
//
LOCUS       DASWNE010000958         2368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_315818, whole genome shotgun sequence.
ACCESSION   DASWNE010000958 DASWNE010000000
VERSION     DASWNE010000958.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315818"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="VGB68_18690"
     CDS             complement(<1..1040)
                     /locus_tag="VGB68_18690"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEY0051331.1"
                     /translation="MNIPEFTEIIEPNDQTEARREHLEKLRALVGNVYPNKFDRSRIT
                     GAEDTISNVLAFEPVAQIVKELKDNTPEGEKPAPELKEQLNTKLREFGNARVSGRLTT
                     TPRTMGKAAFVHLSDGVNRLQIYVRRDDIKGIAGDGKNSEVNGWDLFKLLDGGDFVGV
                     GGFLFLTNTGELSVHVETIQFLSKALLPMPDKLHGINDPELQRRFRYADLIASSLQVE
                     REPGELTTREVFERRARLVSGMRRYLDEHGYIEVETPMLTPKATGAAAKPFETFHEAL
                     KIPLYARIAPELYLKRLTVGGFEKVYELNRNFRNEGLSQKHNPEFTMLEFYCAYMDVN
                     GMMDFAETMIKES"
     gene            1174..>2368
                     /locus_tag="VGB68_18695"
     CDS             1174..>2368
                     /locus_tag="VGB68_18695"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051332.1"
                     /translation="MISNIMSFSTEARTELNRLRDEIKRKTRVISLGGLTSVSAKAFV
                     LSQLQRETGKTFAVVAETNKELETWAGDLKFWSDQPSAVSRQPSEISALSTQHSALLT
                     LPSFETDVYSGISPHAETLEERALTLWKLTHAKPQFVLTSAKALITKTVAPQEIKNYG
                     AFLRRDEDFAPETLIRNLTSCGYVREEPIYNYGQFSMRGGIIDIWSPDAENPVRIEFF
                     GDTVDSIREFDAETQLSVNQIREVRLAPMREFCANEKDFKDWAFFARDRFSDEKFQRN
                     LKDRTDFADEGETFSGWEFLISLANPRKSNLFEYLSETIFVIDEPIVIEQTLASFYEN
                     LANRYAETQAADDIALAPEELFLSVEQLRDCLSRNNRVELRALGLAAAETDEQFQISD
                     SKLEFE"
BASE COUNT          564 a          611 c          598 g          595 t
ORIGIN      
        1 gattctttta tcatcgtttc ggcaaaatcc atcatgccgt tgacgtccat ataggcgcag
       61 taaaattcga gcatcgtgaa ttcgggattg tgtttttgcg acaagccttc gttgcggaaa
      121 ttgcggttca gctcgtaaac cttttcgaaa ccgccgaccg ttaatctttt cagataaagc
      181 tccggcgcga tgcgggcgta aagcggaatt ttcaaggctt cgtgaaacgt ctcgaaaggt
      241 ttcgccgccg cgccggtcgc tttcggcgtg agcatcggcg tttcgacttc gatgtagccg
      301 tgttcgtcca gataacggcg cattccgcta accaggcggg cgcggcgctc gaaaacctcg
      361 cgcgtcgtca attcgcccgg ctcgcgctcg acctgcaggc tcgacgcaat taaatcggcg
      421 tagcgaaaac gccgctgcaa ttccgggtcg ttgatgccgt gcagtttgtc gggcatcggc
      481 aaaagggctt tcgacaaaaa ctgaatcgtc tcgacatgga cacttaactc gccggtattc
      541 gtcaaaaaaa gaaaaccgcc gacgccgaca aaatccccgc cgtccagcag cttaaataaa
      601 tcccaaccgt taacttcaga atttttcccg tcgccggcaa ttccctttat atcgtcgcgg
      661 cgaacgtaaa tctgcaagcg gttgacgcca tccgacaaat gaacgaacgc cgctttgccc
      721 atcgtgcgcg gagtcgtcgt caatctgccc gaaactctgg catttccaaa ttctctgagc
      781 ttcgtattaa gttgttcctt caattccggc gcgggttttt cgccttccgg cgtgttgtct
      841 ttaagttctt tgacgatttg cgctaccggc tcaaaagcca gaacatttga aatcgtatct
      901 tctgcgcccg tgattcggct gcggtcgaat ttgttcggat aaacgtttcc gaccagagcg
      961 cgcagttttt ccagatgctc gcgccgcgct tcggtctggt cgttcggctc gataatttcg
     1021 gtaaattcgg gaatattcat aatgtttttg ctgacagaaa agaaattata aaccaccgta
     1081 aagcaattac gaattacgaa ttacgaatta gaagaaaatt tctttcattc gtaattcgta
     1141 atttgtaatt cgtaattcct aatgtggtaa tctgtgattt caaacattat gagcttttcg
     1201 accgaagcgc gaacagaatt aaatcgtctg cgcgacgaaa taaagcgcaa aacgcgtgtt
     1261 atctcgctcg gcgggctgac ttccgtttcc gccaaagcct ttgttttatc gcaattacag
     1321 cgcgagaccg gaaaaacttt cgccgtcgtc gccgaaacga acaaggagct ggagacctgg
     1381 gcgggcgatt tgaagttttg gagcgatcag ccgtcagccg tcagccgtca gccaagcgaa
     1441 atctcagcac tcagcactca gcactcagca cttttaactt tgccttcgtt tgagaccgac
     1501 gtttattccg gcatctcgcc gcacgccgag acgctggaag aacgcgcttt gacgctctgg
     1561 aaattgacgc acgcgaaacc gcagttcgtt ttgacttccg ctaaagcttt gattacaaaa
     1621 acggtcgcgc cgcaggaaat aaaaaattac ggcgcgtttc tgcgccgcga cgaggatttt
     1681 gcgcccgaaa cattgattcg taatttaacg agctgcggct acgtgcgcga agagccgatt
     1741 tataattacg ggcagttttc gatgcgcggc ggcatcatcg acatctggtc gcccgacgcg
     1801 gaaaatcccg tccgcatcga atttttcggc gatacggtcg attcgattcg ggaattcgac
     1861 gccgaaacgc agctttccgt caatcaaatc cgcgaagtcc ggctcgcgcc gatgcgcgag
     1921 ttttgcgcga atgaaaaaga ttttaaagac tgggcttttt tcgcccgcga ccggttttcg
     1981 gatgaaaaat ttcagcgaaa tctgaaagac cgcacggatt ttgcggacga aggcgagacg
     2041 ttttcgggtt gggaattttt gatttcgctc gcaaatccgc gaaaatcgaa tttgtttgaa
     2101 tacctgtcgg aaacgatttt cgtgattgac gagccgattg ttatcgagca aactctggcg
     2161 agtttttacg aaaatctggc gaatcgctac gccgaaacgc aggcggcgga cgacatcgcg
     2221 ctcgcgccgg aagaactgtt tttgagcgtc gagcagctgc gcgattgttt gagccggaac
     2281 aatcgcgtcg agctgcgcgc gctcggactc gccgccgccg aaaccgacga gcaatttcag
     2341 atttcggatt ccaaattgga atttgaga
//
LOCUS       DASWNE010000959         3344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_31498, whole genome shotgun sequence.
ACCESSION   DASWNE010000959 DASWNE010000000
VERSION     DASWNE010000959.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31498"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VGB68_18700"
     CDS             <1..208
                     /locus_tag="VGB68_18700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051333.1"
                     /translation="TNYPKCDVVFWDKPVTEPCPQCNASFLLEKTTKKDGTIRYCGKC
                     DYKTAAAGDAPVSTEAKDAVSLAS"
     gene            300..878
                     /locus_tag="VGB68_18705"
     CDS             300..878
                     /locus_tag="VGB68_18705"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY0051334.1"
                     /translation="MNEHLQFLQAFLKNPFYVGAISPSSPDLARAMIEGVQPDENNVV
                     LELGVGTGAITKFLREAVPDEKSYLGIEISQEFVEKLKADFSDLLIVCGNAGEACEIH
                     KNTGLGKVKYIVSGLPFASLPKDVSESILREVDKFMAAGCLFRTFQYAHAYRLPPAVK
                     FRKYMEERYGKVRRSSLVLKNVPPAYTLTWKT"
     gene            1044..2756
                     /locus_tag="VGB68_18710"
     CDS             1044..2756
                     /locus_tag="VGB68_18710"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0051335.1"
                     /translation="MLRRKLTVVFLLYAIFLPGLVFGQTPATAPATTTTAPTTTAPAV
                     KVYTAPKETIDRIKEEGLKNSQVMQTLSYLTDVIGGRLTNSPNMKRSNEWTRDMMKKW
                     GLQNAAAEPWGTFGRGWSLKSFSAQVVEPQVFPVIAYPKAWSPSTKGAVTSEVIYLDV
                     KSEADFAKYKGKIAGKIVLISGTRELKADFKGMGTRRTDEELAKMASAPDPALVPRPQ
                     QNQNQPPTAQQIERLQRFITSLKAFNFLVEEGAAAVIDNSFEGSGGTLFVQGANLAQE
                     IPANPQDLFRRNPNRLSPYQKEAEAKMIPQLTMASEDYNRLFRMIQQGVTPRMTLDVQ
                     TQYHDEDLQGYNTVAEIPGTDPTLKDEIVMLGGHMDSWHSSTGATDNAAGVAVAMEAV
                     RILQTLGLKPRRTIRVALWSGEEQGLLGSRGYVKNHFGSWQGEGEARKLETKPAYDKF
                     SAYYNLDNGTGRIRGVYLQGNSAVAPIFRSWLEPFKELGATTLTLSNTGGTDHLAFDA
                     IGLPGFQFIQDEIEYDARTHHSNQDNYDRIQAEDMKQAATIMAAFVYQTAMMDEKIPR
                     KTVK"
     gene            complement(2868..>3344)
                     /locus_tag="VGB68_18715"
     CDS             complement(2868..>3344)
                     /locus_tag="VGB68_18715"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0051336.1"
                     /translation="LFMKMEIIQAETPEAIEQARLLFREYEAWLGMSLCFQNFEEEVA
                     NLPGRYAAPDGRLFLAFVDENLAGCIALRKLEEGVCEMKRLFVREDFRGRKTGIILIE
                     KLIEEAKAIGYQKMRLDTYPTKMANAVKLYESHGFREIPPYYDNPYSGILYLEKEL"
BASE COUNT          863 a          838 c          863 g          780 t
ORIGIN      
        1 cacgaattat ccgaaatgcg acgtcgtttt ctgggataag cccgtcaccg agccgtgccc
       61 gcaatgcaac gcgtcgtttt tgctggaaaa aacgacgaaa aaagacggca cgattcgcta
      121 ctgcggcaaa tgcgattaca aaactgcggc ggcgggcgat gcgcccgtct cgaccgaagc
      181 aaaagatgcc gttagtttag cgagctaatt ggaaacaaca gattaacgcg gctttacacg
      241 gattatttta ttctgtgcta atctgtgtta atccgttgtt tattttaccg caaagattta
      301 tgaatgagca ccttcaattt ctccaggcat ttttaaaaaa tccgttttac gtcggcgcga
      361 tttcgcctag ctcgcccgat ctggcgcggg cgatgattga aggtgttcag ccggatgaaa
      421 acaacgtggt tctcgaactc ggcgtcggca cgggcgcgat tacgaaattt ttgcgcgagg
      481 ctgtgccgga cgaaaaatcc tatctcggaa tcgaaatcag ccaggagttt gtcgaaaaac
      541 tgaaagcgga tttttccgac ctgctgattg tttgcggcaa tgcgggcgag gcttgcgaaa
      601 ttcataaaaa caccgggttg ggaaaagtaa aatatatcgt ttccggtttg ccgttcgctt
      661 cgctgccgaa agatgtgagc gagtcgattc tgcgcgaagt cgataaattt atggcggcgg
      721 gctgcctgtt tcgcaccttt caatatgctc acgcttaccg gctgccgccc gccgttaaat
      781 tccgcaaata tatggaagag cgttacggga aagtccggcg cagctcgctc gtcctgaaaa
      841 acgtgccgcc ggcttacacg ctgacgtgga aaacgtaaaa actgatagtt gataactgat
      901 aactgatagc gaaagcaaaa caactatcag ttatcagtta tcaactatca gctattttga
      961 aaccttttac ataaatctgc tgtaataagt atcagcaaac gttctgcaaa ttcaaatcaa
     1021 tattttccaa ggagtattag aagatgttac gacgtaaact gacagtcgtt tttctgcttt
     1081 acgcaatttt tctaccgggt ttagttttcg ggcagacacc tgcgaccgcg cccgcgacga
     1141 caaccaccgc gccaaccacc actgctccgg cggtaaaagt ttataccgcg ccgaaagaga
     1201 caatcgaccg aatcaaagaa gaaggattga aaaattcgca ggtaatgcag actttaagtt
     1261 acctgaccga cgttatcggc ggacggctga ccaactcgcc gaatatgaag cgctccaatg
     1321 aatggacgcg cgatatgatg aaaaaatggg gcttgcaaaa cgccgctgcc gagccttggg
     1381 gaacattcgg acgcggctgg tcgctgaaat ctttttcggc gcaggtcgtc gagccgcagg
     1441 tttttcccgt tatcgcttac ccgaaagcgt ggtcgccttc gaccaagggc gcggtgacga
     1501 gcgaggtcat ttatctggac gtcaaatcgg aagccgattt tgccaaatac aaaggcaaga
     1561 tagccggtaa aatcgtgttg atttccggca cgcgcgagct gaaagccgat tttaagggaa
     1621 tgggaacgcg gcgcaccgac gaggagctgg cgaaaatggc aagcgcgccc gacccggcgc
     1681 tcgtgccgcg cccgcagcaa aatcaaaatc aaccgccgac ggcgcagcag atcgaacgcc
     1741 tgcagcgatt tataaccagc ctgaaagcct ttaatttttt ggttgaagaa ggcgcggcgg
     1801 cggtcatcga caacagtttt gaaggaagcg gcggaacgct tttcgtgcag ggcgcaaatc
     1861 tcgcgcagga aattcccgcc aatccgcaag accttttccg acgcaatccc aatcgcctgt
     1921 cgccgtacca gaaagaagcc gaagcgaaaa tgattccgca gctgacgatg gcgagcgaag
     1981 attacaatcg cctgtttcgt atgattcagc agggcgtgac gccgcgaatg acgctcgacg
     2041 tgcagacgca gtatcacgac gaagatttgc agggctacaa caccgtcgcc gaaattcccg
     2101 gcaccgaccc gacgctcaaa gacgaaatcg tgatgctcgg cggtcatatg gattcgtggc
     2161 attcatcgac cggcgcaacc gataacgccg ccggcgtcgc cgtcgcgatg gaagccgtgc
     2221 gcattctgca aacgctcggt ttaaaaccgc gccgcacgat tcgcgtggcg ctctggtcgg
     2281 gcgaagaaca gggactttta ggctcgcgcg gctatgtgaa aaatcatttc ggctcgtggc
     2341 agggcgaggg cgaagccaga aagcttgaga cgaaacccgc ttacgacaag ttttccgctt
     2401 attacaatct cgacaacgga acgggcagga ttcgcggcgt ttatttgcag ggaaacagcg
     2461 cggtcgcgcc gattttccgt tcgtggctcg aaccgtttaa agaattgggc gcgacgactt
     2521 tgacgctctc gaatacgggc ggaacagacc atctggcgtt tgacgccatc gggcttcccg
     2581 gtttccagtt tatccaggac gaaatcgaat acgatgccag aacgcatcac tcgaatcagg
     2641 ataactacga ccgcatccaa gccgaagata tgaaacaggc ggcgacgatt atggcggcgt
     2701 ttgtttatca aacggcgatg atggacgaga aaatcccgcg caaaacggtg aagtaaaata
     2761 agtaaatgag gtaaaaggca aaaggcaaaa gtttggaagt ttcttccggc ttttgccttt
     2821 tgctcattcg aaggttttgg gcgcgctttt tatttttgct ttttgcctta taactctttt
     2881 tccagataaa gaattccgct gtacggattg tcataataag gcggaatttc ccggaagccg
     2941 tgcgattcgt aaagctttac ggcgttcgcc attttcgtcg ggtaagtgtc gagccgcatt
     3001 ttttgatagc cgattgcttt ggcttcctca atcaattttt caatcagaat aatgcctgtt
     3061 ttacgcccgc gaaaatcttc cctgacgaat aatcttttca tttcgcagac gccttcttcc
     3121 agttttctga gcgcgataca tccggcgaga ttttcgtcga cgaaagcgag aaataaacgc
     3181 ccgtccggcg cggcgtatct gcccggcaaa ttagcgactt cctcttcgaa attctgaaaa
     3241 caaaggctca ttcccaacca cgcttcgtat tctcgaaaca acaatcgcgc ctgttcgatg
     3301 gcttcgggcg tctcggcttg aataatttcc attttcataa aaag
//
LOCUS       DASWNE010000960         1599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_279679, whole genome shotgun sequence.
ACCESSION   DASWNE010000960 DASWNE010000000
VERSION     DASWNE010000960.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279679"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            303..>1599
                     /locus_tag="VGB68_18720"
     CDS             303..>1599
                     /locus_tag="VGB68_18720"
                     /inference="COORDINATES: protein motif:HMM:NF016330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HEY0051337.1"
                     /translation="MPIFWTSEQVIAQASDDSSVKAGRSLAIISKWVSHGYDEHAVWG
                     ECKGSGQRPYQVTIELSEPAFKCSCPSRKFPCKHGLGLFLLYANNQLKENSRPEFCSE
                     WLAKRSQTQERKKEKSEAEPTPEKAEKREKAKINRIGEREAKVAAGLRELEIWIADMI
                     RQGLATVQSRPTEYWEKMAKRLIDAQAPNAARLVLQMSSLIYQKNKYQEKLAEKLLEH
                     LSGIYLICESYRRINELPAAIQADVKTAIGFTVKEEELQTVETIEDVWQIHGIRVFEE
                     EKLRVQRVWLSGENSRRSALILNFAFRNQPLDVSFAAGTKIKAELAFYPGNSAQRAFL
                     KTRRESQNLEMADGCETFTEFLRNYSEALAKNVWLETFPAMLKNVVPVRRDSIWFLRD
                     KEGKMLRIDADFGAIWKLFAICGNQPAAIFAEWDGETFLP"
BASE COUNT          473 a          350 c          398 g          378 t
ORIGIN      
        1 aatttaccga aagcgaatca gtcaaattat tttcgggttt aataacgctt tagaagaaaa
       61 attcgcgctt taattgaaaa acattagtgc acaaagcgaa aatttccggc attttcagcg
      121 cgtataagga aaatttccgc cgcgaataaa atctttgtag aatgccggaa aattttctat
      181 aatttatgcc ggaagttaac tcaaaaaaat ctccagacat ctaatatcaa ttttataagc
      241 gttttaaact atcggattat taatttttaa ttttagatac aaagctcgct tttcatattc
      301 gaatgccaat attttggact tctgagcagg taattgcgca agcgtctgac gattcgtccg
      361 tgaaagccgg acggagtctg gcgattattt ccaaatgggt ttcccacggc tacgacgagc
      421 acgccgtctg gggcgaatgt aaaggaagcg gacaaagacc ttaccaggtt acaatcgagc
      481 tttccgagcc ggctttcaag tgcagttgtc cgagccgcaa atttccctgt aaacacggac
      541 tcggattatt tttgctttac gcaaacaatc aactgaagga aaacagccga ccggaattct
      601 gttccgaatg gctggctaag cgcagccaaa ctcaggaacg aaaaaaagaa aaaagtgaag
      661 ccgaaccgac gccggaaaaa gcggaaaagc gcgaaaaagc caaaatcaac cgcatcgggg
      721 agcgagaggc aaaagtcgcc gccggattgc gggagctcga aatctggatt gccgatatga
      781 ttcggcaggg actggcgaca gtccagagtc gcccgaccga atattgggaa aaaatggcga
      841 aacgcctgat tgacgcgcaa gctccgaatg cggcgcggct cgtccttcaa atgtcgagcc
      901 ttatttacca aaaaaataaa tatcaggaaa agctggcgga aaaactgctg gaacatttga
      961 gcggaattta tttgatttgc gagagttacc gccgcataaa cgaattgcct gccgcgattc
     1021 aagccgacgt taaaacggca atcgggttta cggtcaaaga agaagagctg caaacggtcg
     1081 aaacaatcga agatgtctgg caaattcacg gaattcgtgt tttcgaagaa gaaaagctcc
     1141 gcgtgcagcg cgtttggctt tcgggtgaaa acagccggcg cagcgctttg atattgaatt
     1201 tcgctttccg gaatcaaccg cttgacgtta gtttcgccgc cggcacaaaa atcaaagccg
     1261 agctcgcttt ttatcccgga aattctgcgc agcgggcttt tttaaaaacg cgccgggaat
     1321 cgcaaaatct tgaaatggcg gacggttgcg aaacttttac ggagtttttg cggaattatt
     1381 cggaagctct ggcgaaaaac gtctggctcg aaacgtttcc ggcaatgttg aaaaatgtcg
     1441 ttcccgtgcg gcgcgactcg atttggttcc tgcgcgataa agaaggaaaa atgctgagaa
     1501 tagatgccga tttcggcgca atctggaagc tgttcgccat ctgcggcaat cagcccgcgg
     1561 cgatttttgc cgaatgggac ggggaaacgt ttctgcctt
//
LOCUS       DASWNE010000961         1783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_315868, whole genome shotgun sequence.
ACCESSION   DASWNE010000961 DASWNE010000000
VERSION     DASWNE010000961.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315868"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VGB68_18725"
     CDS             <1..618
                     /locus_tag="VGB68_18725"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEY0051338.1"
                     /translation="NSLGNIPEVTVNFPNTELGNQLKQIARLIRGRSVLAVNRQIFFV
                     ILDGFDTHTTQLAEQDRLLSQLSQASRAFYEEMVVQGLQNQVTQFTLSDFNRTFNPGG
                     SGALVGSDHAWANHMFVIGGGVLGGDFYGMNTSNGTPFPTLVQNGPDDADGGSNARGR
                     WIPTTSVEQYAATLAKWFGLPDEDRSYVFPNLANFPTTNLGFMSP"
     gene            complement(815..>1783)
                     /gene="ribD"
                     /locus_tag="VGB68_18730"
     CDS             complement(815..>1783)
                     /gene="ribD"
                     /locus_tag="VGB68_18730"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEY0051339.1"
                     /translation="YENVTHAEVLALNQADGRGKGGTAYVSLEPHAHRGRTAPCTEAL
                     INAGIKRVVAPIEDPNPLVSGKGFEQLRLNGIEVVTGILKEEAARLNEKFCHWHRAKR
                     PFVHLKMAISLDGRIATRTGDSRWITGEESRAKAHELRHEYDAILVGANTVTVDNPSL
                     TDRSGKKRRRPLVRVVVDNSLKISPASNLVLTAGEFPTLIFTDNRDADKIADLRSEGV
                     EVLQIAEGGRNLQGVLYELGRRDLQSVLIEGGARVAGSFYDAKLIDKISFFIAPIVIG
                     GEDAPTAIGGSGASRLTGAMHLENIEINGRGADLEVTGYPARQKEN"
BASE COUNT          386 a          542 c          427 g          428 t
ORIGIN      
        1 aattctttgg gaaatattcc cgaagtgacc gtcaattttc cgaacaccga gctcggcaac
       61 cagcttaaac aaatcgcgcg tcttattcgc gggcgcagcg tgctggctgt caaccgccag
      121 attttctttg tcattctcga cggcttcgac acgcacacca cgcagctcgc cgagcaggac
      181 agacttttgt cgcagctgag ccaggcatcg cgggcgtttt atgaagaaat ggtcgttcag
      241 ggattgcaga atcaggtgac tcaatttact ttgtcggatt tcaatcgcac gtttaatccg
      301 ggcggctcgg gcgcgctggt cggcagcgac cacgcctggg caaatcatat gttcgtcatc
      361 ggcggcggcg tcctcggcgg cgatttttac ggaatgaata cctcgaacgg tacgcctttt
      421 ccgacgctcg tccaaaacgg acccgacgac gccgacggcg gctcgaatgc gcgcgggcgc
      481 tggattccga cgacatcggt cgagcaatac gccgcgacgc tggcaaaatg gttcgggctg
      541 ccggacgaag accgaagcta tgtctttccg aatctggcaa actttccgac aacgaatctg
      601 ggctttatga gcccttgata aaacttatct gtaaagcgaa gataattttt caatgcgatt
      661 ccaattaaaa aagaacagcc gcacgcaaca ttgcgtgcgg ctgtccggct ttttatttcg
      721 tattagaaaa ccgctttctt ttaccggaat tccgccttcg ccgtgaaaac aaatctccga
      781 taaatattat ctcttcgtac gagattttgc ggctttaatt ttctttttgc cgcgccggat
      841 agccggtcac ttccaaatcc gcgccgcgcc cgttgatttc gatattttcc agatgcatcg
      901 cgcccgtcag acgcgacgcg ccgctgccgc cgattgccgt cggcgcgtct tcgccgccga
      961 taacgatggg cgcgataaaa aacgagattt tatcaatcaa tttcgcgtcg taaaacgagc
     1021 ctgccacgcg cgctccgccc tcaatcaaaa cgctctgcaa atcgcgacgc ccaagctcat
     1081 acaaaacgcc ctgcaaattc cttccgcctt ccgcaatctg caaaacctcg acgccttcgc
     1141 ttcgcaaatc ggcgattttg tccgcgtccc tgttatcggt aaaaatcaat gtcggaaact
     1201 ctccggcggt caaaaccagg tttgaagccg gcgagatttt caaagaatta tcgaccacca
     1261 ctcgcaccag cgggcgacgt cgttttttcc cgctcctgtc cgtcagactg ggattatcga
     1321 ccgtcacggt atttgcgccg actaaaatag cgtcgtactc gtgtcgcaac tcgtgcgctt
     1381 tcgcccgcga ttcttcgccc gtaatccagc gcgaatcgcc cgtccgcgtc gcaatcctgc
     1441 cgtccagcga aatcgccatt ttcaaatgca cgaacggacg ttttgcccgg tgccagtgac
     1501 aaaatttttc gttcagccgc gccgcttctt cctttagaat tcccgtcacg acttcgattc
     1561 cattcagccg caactgttca aagcctttcc ccgaaacaag cgggttcggg tcttcaatcg
     1621 gcgcgacgac tcgtttaatt cccgcgttta tcaaagcctc ggtgcagggc gcggttcttc
     1681 cgcgatgcgc gtgcggctca agcgaaacat aagccgtccc gcctttccct cgcccgtccg
     1741 cctgattcaa cgccagaacc tcggcgtgcg tcacattttc ata
//
LOCUS       DASWNE010000962         3022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_136249, whole genome shotgun sequence.
ACCESSION   DASWNE010000962 DASWNE010000000
VERSION     DASWNE010000962.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136249"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            279..1007
                     /locus_tag="VGB68_18735"
     CDS             279..1007
                     /locus_tag="VGB68_18735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051340.1"
                     /translation="MSPIVFALSFLLIASAGVFAQSPAQEVSETDGVPVIIKHLPEWE
                     NVQNKAALIKNTNDLRTALGDRPVFDLIDFEGGTEAVAAPYEAGKLLIVEYNTPQGSI
                     ETDEKVKQRLSESAPNPPIYYRRVGNYNVFVFDAADETSANALLDQIKYEKIVQWLGE
                     DPFLYERQQRSYALTLTEIFFATVMAIVSGIGLAILAGIGVGIAFFYMRKKQRDDMES
                     FSDAGGMLRLNLDELTPDIPLEEK"
     gene            complement(1121..2245)
                     /locus_tag="VGB68_18740"
     CDS             complement(1121..2245)
                     /locus_tag="VGB68_18740"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY0051341.1"
                     /translation="MNENIRKLFPALQKYTYLNSAAVSPMPTVAVEAAYSQLKDASEN
                     GTINYLDWIQTKQRARELIAEMLKVRSEQIAFMRNTSDGFSAVANGLDWAEGDNVVTF
                     EREFPANFYPWRMIRDKFGVELRLCPERDGRIDLDEFLSLIDSNTRLVSISAVQYASG
                     FRADLERIGRVAREADALFAVDIIQGFGAMPFDLPAQFVDIAAGASHKWLCSPEGCGI
                     LYLSDRAHRRVEPTFVGWISVEVPWNFEDSEQAWKPNALALESGTGGSSLFYGLEQSL
                     KLLRETGAEDIRNYLENLTDYLCELLRAKNYEIVSSRAKSEKSQIVCILPKANQTSAE
                     LAENLQNENIIVSPRGERIRIAPHFFNNRADIERLIEALP"
     gene            2455..>3022
                     /gene="lepB"
                     /locus_tag="VGB68_18745"
     CDS             2455..>3022
                     /gene="lepB"
                     /locus_tag="VGB68_18745"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY0051342.1"
                     /translation="MFDFSKKRQPEFGAVAIDDDRDIEVHRLKKESRHGLYSEGLKLV
                     RDIILVLTVFILLGVFVAQPVVVEGTSMLPELHDGERLLVNKLVYYKFKDVSWGHIER
                     GDIVVFWYPKEPDKSYVKRIIGLPGEMVEVRNGVVYINGEEMREPYLSSEHNQSLGSF
                     PPKKVEEHYYFVMGDNRDNSSDSRYWGLV"
BASE COUNT          783 a          736 c          727 g          776 t
ORIGIN      
        1 gcgtgccata aactatattc gtaggtttat aatttttgga tgcttataaa tgccttttta
       61 tcacaatatc ttgggcatca tatcttacac gccgaacatc ttaaaaagtt gcaaccgaaa
      121 ccgtgtagac gataacgcat atgatacacg tctgtttgtg tatctattca ttacagttat
      181 acgaccgttt atcataaatg tttaattttc acctcaataa tgctagtatc gaaatttccg
      241 aacagcgcga agaaatttca atgcggagaa gatttttaat gagtccgatt gtatttgctt
      301 tgagttttct cttgatagct tcagcgggcg tttttgccca gtcgccggca caggaagttt
      361 ccgaaaccga cggagtcccc gttatcatca agcatctgcc cgaatgggaa aacgtgcaga
      421 acaaagccgc tttaataaaa aacacaaacg atttgcgcac cgcgctcggc gaccgaccgg
      481 ttttcgattt gattgatttc gagggcggca cggaagccgt cgccgcgcct tatgaagcgg
      541 gaaagctttt gattgtcgaa tacaacacgc cgcaaggctc gattgaaacg gacgaaaagg
      601 tcaaacaacg attgagcgaa tccgcgccga atcccccgat ttattatcgc cgcgtcggaa
      661 attacaacgt ctttgtcttt gacgctgcgg acgaaacatc ggcaaatgct ctgcttgacc
      721 agattaaata cgaaaaaatc gtgcagtggc tcggcgaaga cccgtttttg tatgaacggc
      781 agcagcgaag ctacgctctg acgctgaccg agatattttt cgcgaccgtt atggcaatcg
      841 tttccggcat cggtctggca attctggcgg gcatcggcgt cggcatagcg tttttctata
      901 tgcggaaaaa acagcgcgac gatatggaat cgttttccga cgcgggcgga atgctgcgcc
      961 tgaatctgga cgaactgacg ccggacattc ctttagaaga aaaatagccg cgaaaaaaca
     1021 aaaggcgcaa agaaattaag ccgcgcaggg gcgcgaaaaa gcacgaaata atttttgttt
     1081 tatattcgtg agctttcgcg tctctgcgcg gctgattctt ttacggtaaa gcttcaatca
     1141 atctttcaat atcggcgcga ttattaaaaa aatgcggcgc gatgcggatt cgctcgccgc
     1201 gcgggctgac gattatgttt tcgttttgca gattttccgc cagctcggcg gaagtttgat
     1261 tcgctttcgg caaaatgcag acaatctgcg atttttcgct tttcgcgcgc gagctgacga
     1321 tttcgtaatt tttcgcccgc aaaagctcgc acagataatc cgtcaaattc tccagataat
     1381 tccggatgtc ttccgctccg gtttcgcgca aaagtttcag gctctgttcc aaaccgtaaa
     1441 ataagctcga accgcccgtt cccgattcta aggcgagcgc attaggtttc caagcctgct
     1501 cggaatcttc aaaattccac ggcacttcga cgcttatcca gccgacaaaa gtcggctcga
     1561 cgcgccggtg cgcgcggtcg gaaagatata aaattccgca gccttccggc gagcagagcc
     1621 atttgtggct cgcgcccgcc gcgatatcga caaattgcgc gggcaaatca aacggcatcg
     1681 cgccgaagcc ctgaataata tcgactgcga aaagcgcgtc cgcctcgcgc gcgacgcgtc
     1741 cgattctttc caaatcggcg cgaaagcccg atgcgtattg aaccgcgctg attgaaacca
     1801 gacgcgtgtt tgaatcgatt aaacttaaaa attcgtccaa atcaatccgc ccgtcacgtt
     1861 ccggacaaag ccgcagctcg acgccgaact tatctctaat cattcgccac ggataaaaat
     1921 tggcgggaaa ttcgcgctcg aaagtgacga cgttatcgcc ttccgcccaa tccaagccgt
     1981 tggcgacggc ggaaaaaccg tccgaagtat tgcgcataaa cgcgatttgc tcggagcgca
     2041 ctttcagcat ttcggcaatt agttcgcgcg ctcgctgttt ggtctgaatc caatccagat
     2101 aattaatcgt gccgttttcc gaagcgtcct taagctgtga atatgcggct tcgaccgcga
     2161 ccgtcggcat cggcgaaacc gccgcgctgt ttaaataagt atatttttga agggcgggaa
     2221 ataatttgcg aatattttca ttcataactt ctaaagcgaa aaagtgaaat agtgaaatag
     2281 tgaaaaagcc gattgattag taaacggcaa aactttttta cattttcacg ttttcgcgtt
     2341 ttcactttct cgttcttttg acaaaccaga ttgttacagc taattttgaa atggtttaga
     2401 aagaagtttt gcgccaacgg caaaggcttt ccaaacatcg aatctgcttg aattatgttc
     2461 gatttctcca aaaaaagaca gcctgagttc ggcgccgtgg cgattgacga cgaccgcgac
     2521 attgaagtgc atcgcctgaa aaaagaatcg cggcacggtt tgtattccga aggcttaaag
     2581 ctcgtccgcg acatcattct cgtgttgacg gtttttattc tactcggcgt ttttgtcgcg
     2641 cagccggtcg tcgtcgaagg aacctcgatg ctgccggagc ttcacgacgg cgagcgcctg
     2701 ctggtcaaca agcttgttta ttataaattt aaggacgtca gctggggaca tatcgagcgc
     2761 ggcgatattg tcgttttctg gtatccgaaa gagccggata aaagctacgt caaacgcatt
     2821 atcggtctgc cgggcgaaat ggtcgaggtt cgcaacggcg tggtttatat taacggcgaa
     2881 gaaatgcgcg agccttactt aagctccgag cacaatcagt ctttaggcag ttttccgccg
     2941 aaaaaagtcg aagaacatta ctatttcgtg atgggcgaca atcgcgataa ttcttccgac
     3001 tcgcgctact ggggcttggt tc
//
LOCUS       DASWNE010000963         3217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_525544, whole genome shotgun sequence.
ACCESSION   DASWNE010000963 DASWNE010000000
VERSION     DASWNE010000963.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525544"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2306)
                     /locus_tag="VGB68_18750"
     CDS             complement(<1..2306)
                     /locus_tag="VGB68_18750"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEY0051343.1"
                     /translation="MSSTNLQTAVGGKENKAGEPDNLQIFAEFNRWIERHLERDFPSG
                     DEHLRLGTSLAAKRRLAMTDLIRTDPERALSFAVAEELIQRLPSAVSANLEKHINDRG
                     DFTVLVFDEDFAGAAASHRTERRVKIGNISYRAFVYGRKQTMTTKNDIPVRGIVLDSL
                     MAVAENPVRKLDASEYDLRNVDIERLSADGVAAQVGGEIIYFADEREFVNFETELIDW
                     ERKIAPARSARQNPENAASPWTEGTKRLLFIRVDYSDRPGEPIDRTNQPLTPARAQAL
                     MDTQVSPFYVSNSYQKTSLQTTVTTVVRMPQPQSFYSPENLFALLVDARNAARAAGFN
                     TDEYDLDIVAFSYTPVLNFSGIAVVGAKGALLNGSFTFKTVTHELGHEYGLIHANLWR
                     TGDGTIIGQGSNVEYGDNYDMMGRAAQPFSHFSAAYKRRLDWLPESNVKTVTASGIHR
                     LFASDSPAVAKGIQLLKIPKHAARNYLVEYRQSYSSVPGLSNAALLRWDLLDAQGRFQ
                     TQMLDMTPATTDINVTDEMLPIGQTFTDSESGIKITILGKGNTAPESLDVRVEFLNSR
                     PITPFDFDGDGKADVSVFRPSNGVWYRLNSGANNSFVAAQFGSAQDKPAPADYDGDGR
                     TDIAVFRPSNGTWYLQQSNAGFSAAEWGASGDIPAPADFDGDGKADYAVYRPSNGVWY
                     LQRSSQGFAAVNFGLPEDIPVASDYDGDGKADIAVYRPSNGGWYRLNSASNQFVAVSF
                     GTGEDKPVPADFDADGKTDIAVFRPSEG"
BASE COUNT          750 a          833 c          793 g          841 t
ORIGIN      
        1 aagccttcag agggtcggaa aaccgcgatg tcggttttac cgtccgcgtc aaagtccgcc
       61 gggacgggtt tgtcctctcc ggtgccgaac gatactgcga cgaattgatt gctcgcgctg
      121 ttcaggcgat accagccgcc gtttgacggt cgataaacgg cgatgtccgc tttgccgtcg
      181 ccgtcgtaat cgctcgccac cggaatatct tccggcaagc cgaaattaac cgcggcaaaa
      241 ccctgactgc ttcgctgcag ataccaaacg ccgtttgacg ggcgataaac cgcgtaatcg
      301 gctttgccgt cgccgtcgaa atcggcgggt gcgggaatgt cgccggacgc gccccattcg
      361 gcggctgaaa atccggcgtt tgattgctgc agataccacg ttccgtttga cgggcggaaa
      421 acggcgatgt cggttctgcc gtcgccgtcg taatccgccg gagcgggttt atcctgcgcc
      481 gacccgaatt gagcggcgac aaaagaattg ttcgcgccgc tgtttaagcg ataccaaacg
      541 ccgtttgaag gtcgaaagac cgaaacgtca gccttgccgt cgccgtcgaa atcaaacggc
      601 gtgatggggc ggctgttaag aaattcgacc cgcacgtcca aactttccgg cgcggtattg
      661 ccttttccca aaatcgtaat tttgatgccg ctttcactgt cggtaaaagt ctgaccgatc
      721 ggcagcattt cgtccgtgac gttgatgtcg gtcgtcgccg gagtcatatc gagcatctgc
      781 gtttgaaatc tcccttgagc gtcgagcaaa tcccatcgga gcagcgcggc gttcgataag
      841 cccggcacgc tgctgtaaga ttgcctgtat tcgaccaggt aatttctggc ggcgtgtttg
      901 ggaattttca gaagctgaat gcctttcgcg acagccggcg aatcggaagc gaataatcgg
      961 tgaatgccgg aagcggtcac cgttttgacg ttcgattcgg gcagccagtc gaggcgtcgt
     1021 ttataagccg cgctgaagtg cgaaaacggt tgcgccgcgc gtcccatcat atcgtaattg
     1081 tcgccgtatt ccacgttgct gccctgcccg ataatcgtcc cgtcgccggt gcgccagaga
     1141 ttggcgtgaa tcaaaccgta ttcgtgtccg agctcgtgcg tgacggtctt aaaggtgaac
     1201 gaaccgttta gcagagcgcc cttcgcgccg acgacggcga tgccggaaaa attcagaacg
     1261 ggcgtatagc tgaacgccac tatgtcgagg tcatattcgt cggtgttaaa tccagccgcc
     1321 cgcgccgcat tacgggcatc gaccaaaagg gcgaataaat tctcgggcga atagaaagat
     1381 tgcggctgcg gcatcctgac aacagtcgta actgtcgttt gcagcgaagt tttctggtag
     1441 gaattgctca cataaaacgg gctgacctgt gtatccatca aagcttgcgc tcgcgcgggc
     1501 gtcagcggct ggttagtgcg gtcgatcggc tcgcccggtc ggtcggaata atccacgcga
     1561 ataaaaagca atctttttgt gccttccgtc cacggcgaag ctgcgttttc cgggttctgg
     1621 cgagcggaac gagccggagc gatttttctc tcccaatcaa tcaattcggt ttcgaaattt
     1681 acaaattccc gttcgtccgc aaaataaatt atttcgccgc cgacctgcgc ggcaacgccg
     1741 tccgcgctta accgctcgat atcgacgttg cgcaaatcgt attcggaagc gtctaatttg
     1801 cgaaccggat tttccgccac cgccatcaaa gagtccaaaa cgattccccg caccggaatg
     1861 tcgtttttcg tggtcattgt ttgttttctg ccgtagacga aagcccggta gctgatattg
     1921 ccgattttca cacgtcgctc agtccggtgg ctggcggcgg ctccggcaaa atcttcgtca
     1981 aaaaccaaaa ccgtaaaatc gccgcggtcg ttaatgtgct tttcgagatt tgcgctgacg
     2041 gctgacggca atcgttgaat cagctcttcc gcgacggcaa aactcagcgc cctttcgggg
     2101 tcagtccgaa tcaaatcggt cattgccagc cggcgcttcg ccgccagact cgtccctaag
     2161 cgaagatgct cgtctccgct cggaaaatcg cgctcgaggt gacgctcaat ccaccgatta
     2221 aactcggcga agatttgtaa attatccggc tcgccggctt tattttcttt cccgccgaca
     2281 gcggtttgca gatttgtaga agacacggat ttttcggctg ccgtgctgac gttttgccaa
     2341 acaaataagc ctgcgcataa taaaaacaat gttatataga gagtcaagac actcaaaatg
     2401 tgttttttcc agttaaattt catatccttt tcgcttaagc gcccgttgtc ggcgtttcta
     2461 aagattcggc atttcttccg ccttcttaaa aatcccacgc cccgcggcac attgtgtcag
     2521 aggctgtttt actttccctt tcgaacaaaa acaggtcggg aaacccggct ctccggctaa
     2581 atttccgagt acgaacttta ttatttgtat aagcccgcga atagctcttt gtgatgagcc
     2641 gtgaatagcc gttaccaaac gtttaattgc aggcaaaaca aatttttaac tattttgtct
     2701 gataaagcgt ttttcccgga atgcccggat aaagcgcgaa atcgggataa actattcaag
     2761 aatgagctgt tactacacaa aatcagctta atgtataaaa tcttttcagg ctttaatttg
     2821 tgccgaatag ttttaattcg tgctgaacag ttaaataggc agcccgtcaa tttcaaaaat
     2881 tcattttcct gatgtttatt attgacgatt caccacgtta tttcacgatt agtcacattc
     2941 cgctgttaaa agaattgccc tgcttactat attggtgcgg tatgccttta gttataaaga
     3001 aagcaatgaa aagccgcaac gcttcgttcc gttttaatac ttcttttgtc tttaaactta
     3061 ataatccttt tctgaccgaa attggagctg agtcataacc gatacattct aacttcggtt
     3121 agcacaattt taagtaattt tattttctcg gcgtattttg cgcttaaata ttaagataaa
     3181 cagtttgact aatattgcct ctcaatctat gatatga
//
LOCUS       DASWNE010000964         2900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_103101, whole genome shotgun sequence.
ACCESSION   DASWNE010000964 DASWNE010000000
VERSION     DASWNE010000964.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103101"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="VGB68_18755"
     CDS             complement(<1..682)
                     /locus_tag="VGB68_18755"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY0051344.1"
                     /translation="MMEKFATGEGTKRFAEKFKDAASGHFRDAQGLTVSSIGIGTYLG
                     DWDAATDESYTSAIARFVERGGNVIDTAANYRFQRSERNIGEALKILGEKGFGRDEIL
                     VCTKGGYLPFDGEPPRDVRAYFQENFVAKGIATFDDLVGGSHCMTPKYLESQVNQSLQ
                     NMRLDSLDVFYIHNPESQLSEIPATAFEARLARAFEKLEELRTNGKLQFYGAATWNGF
                     RVPPSNNGY"
     gene            complement(682..1167)
                     /locus_tag="VGB68_18760"
     CDS             complement(682..1167)
                     /locus_tag="VGB68_18760"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY0051345.1"
                     /translation="MAESLAFSKTKDRKVIYEEIAPQIEALVSGETDLVANLANIAAA
                     LKEAFGFFWVGFYIKKENQLVLSAFQGSIACTRIDFDKGVCGHCYTTRETAIVPDVEK
                     FPGHIACSSLSKSEIVLPIFGAAGEVFGVLDVDSDRLDDFSQADAEGLRKIVDIIERI
                     I"
     gene            1394..2263
                     /locus_tag="VGB68_18765"
     CDS             1394..2263
                     /locus_tag="VGB68_18765"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEY0051346.1"
                     /translation="MLEKELEVAVALAREAGKTILEFYALEIIAEEKLGADNFSEPVT
                     IADKTASRMIVGGLTEAFPADGVLSEEEDDLIEIRASRNRVWMIDPIDGTWGFIKKDG
                     DFGVQIGLTENGEVILGVVFLPVHNTLYYASKGAGAFMVQNDEAPKRLQVSGKTDFTE
                     MLLASSRNHRSPKMHRIIEDFGFRQEIQRGSVGIKIGLIAQQICDLYIHLSPRTKFWD
                     TCAPQIILEEAGGKMTDLFGYPMRYDLHDVQNHNGILATNTAAHEETVQLLKPLLREF
                     GRLRLKAKANENF"
     gene            2380..>2900
                     /locus_tag="VGB68_18770"
     CDS             2380..>2900
                     /locus_tag="VGB68_18770"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEY0051347.1"
                     /translation="MPKKQTSPFDNVAPLDEDTGDLHVVVDTPKGSRNKYEFDEKLGL
                     FKLGGVLAVGHSFPFDFGFIPNTLGGDGDPLDVLVLLDEPAFVGCLIPARLIGVVEAE
                     QTERDGETGRNDRLIAVASNSRVHEDVKSINDLNETLVDEIEHFFVSYNEAKGKKFKP
                     LGRCGAQKARKII"
BASE COUNT          714 a          771 c          722 g          693 t
ORIGIN      
        1 ggtaaccgtt atttgacggc gggacgcgaa agccgttcca cgtcgccgcg ccgtaaaatt
       61 gcagcttgcc gtttgttcga agttcttcga gcttttcaaa cgcccgcgcc agccgcgctt
      121 caaacgcggt tgcgggaatc tcggaaagct gcgattcggg attgtgaatg tagaaaacgt
      181 ccagcgaatc gagccgcata ttttgcagcg attgattgac ctggctttcc aggtatttcg
      241 gcgtcataca atgactgccg ccgaccaaat cgtcaaacgt cgcgatgcct ttagcgacaa
      301 aattttcctg aaaatacgcg cggacgtcgc gcggcggctc gccgtcaaac ggcagataac
      361 cgccttttgt gcaaaccagg atttcgtcgc gcccgaagcc tttttcgcct aaaattttca
      421 gcgcttcccc gatatttcgc tcgctgcgct gaaaacgata attcgccgcc gtgtcgataa
      481 cgtttccgcc gcgctcgaca aagcgcgcaa tcgcgctcgt atagctttcg tcggtcgccg
      541 cgtcccagtc tccgagataa gttccgatgc cgatcgacga aacggtcaag ccctgcgcat
      601 cgcgaaaatg cccgctcgcg gcatctttaa atttttcggc gaagcgcttt gtgccttcgc
      661 ctgtcgcaaa tttttccatc attaaattat tctttcaata atatcgacga tttttcgcaa
      721 accttcggcg tcagcctgcg agaaatcgtc gagccggtcg ctgtcgacgt ccaggacgcc
      781 gaaaacttcg cccgccgcgc cgaaaatcgg caaaacgatt tccgattttg aaagcgagct
      841 gcaggcgata tgaccgggaa atttttcgac atccggtaca atcgccgtct cgcgcgtcgt
      901 gtaacaatgc ccgcagacgc ctttgtcgaa atcaatgcgc gtgcaggcaa tcgagccttg
      961 aaacgcgctc aggacaagct ggttttcctt tttaatataa aatccgaccc agaaaaaacc
     1021 gaacgcttct ttgagcgccg ccgcgatgtt cgccagattt gccaccaaat ccgtttcgcc
     1081 cgaaaccagc gcttcaatct gcggcgcgat ttcctcgtaa attactttcc ggtctttcgt
     1141 ctttgaaaaa gccaaacttt ccgccataag ctcaagttta tcacgaaaaa tttcagccgc
     1201 agagaacaca gagattttta agaaattgaa accaaggatt tacacagatt aacgctcatt
     1261 attaacagga tagataggat ggataggata aaacttttct tttatctatg ttcatccgtg
     1321 taaatccgtg gtttcaattt ccttctctgt gcttctctgt gttctctgtg ttctctgtgg
     1381 taaattttaa gtaatgctgg aaaaagaact cgaagtcgcc gtcgccctcg cgcgcgaggc
     1441 gggcaaaaca atcctggaat tctacgcgct ggaaatcatt gccgaagaaa agctcggcgc
     1501 tgacaatttc tccgagccgg tcacgatagc cgacaaaacc gccagccgga tgattgtcgg
     1561 cggcttgacc gaagcttttc cggcggacgg cgttttgtcc gaagaagaag acgaccttat
     1621 agagatacgc gccagcagaa atcgcgtctg gatgattgac ccgattgacg gaacctgggg
     1681 ctttatcaaa aaagacggtg atttcggcgt gcaaatcggt ttgaccgaaa atggcgaggt
     1741 gattctgggc gtcgtttttc tgccggttca caatacttta tattacgcgt cgaaaggcgc
     1801 gggcgcgttt atggttcaaa acgacgaagc gccgaagcgc ctgcaagttt ccggcaaaac
     1861 ggattttacg gaaatgcttc tggcttcgag ccgcaatcac cgcagcccga aaatgcatcg
     1921 catcatcgaa gatttcggct tccggcagga aatccagcgc ggctcggtcg gcatcaaaat
     1981 cggtttgata gcgcagcaaa tctgcgattt atacattcat ctcagcccgc gcacaaagtt
     2041 ttgggacacc tgcgcgccgc agattattct ggaagaagcg ggcgggaaaa tgaccgacct
     2101 tttcggctat ccgatgcgct atgatttgca cgacgtccag aatcataacg gcattttagc
     2161 cacgaacacg gcggcgcacg aggaaacggt tcagctttta aaaccgctgc tgcgcgagtt
     2221 cggacgcctg cgcctgaaag caaaagcgaa cgaaaacttt taactgcggc gcgcttccgc
     2281 gcatttttta acttcgcgcg cattgcccgg aaagctgaaa atcctgtttt tgaaaattcg
     2341 acaaatatag ttgctaaaaa attttccctt aatcgtttta tgccgaagaa acaaaccagt
     2401 ccttttgata acgtcgcgcc gcttgacgaa gacacgggcg accttcacgt tgtcgtcgat
     2461 acgccgaaag gcagccgcaa caaatacgag tttgacgaaa agctcggtct tttcaaactc
     2521 ggcggcgtgc tggcggtcgg gcattctttt ccgttcgatt tcggttttat tccgaacacg
     2581 ctcggcggcg acggcgaccc gctcgacgtg ctggttttgc tggacgaacc ggcttttgtc
     2641 ggctgtctga ttccggcgcg gctcatcggc gtcgtcgaag ccgagcaaac cgaaagggac
     2701 ggcgaaaccg ggcgcaacga tagattaatc gccgtcgctt ccaattcgcg cgtgcacgaa
     2761 gacgtaaaat caatcaacga tttaaacgaa actctggttg acgagatcga gcattttttc
     2821 gtttcctaca acgaagcaaa aggcaaaaag ttcaaaccgc tcggacgctg cggcgcgcag
     2881 aaggcgcgaa aaattattga
//
LOCUS       DASWNE010000965        16648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_525605, whole genome shotgun sequence.
ACCESSION   DASWNE010000965 DASWNE010000000
VERSION     DASWNE010000965.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 16648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16648
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525605"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4690)
                     /locus_tag="VGB68_18775"
     CDS             complement(<1..4690)
                     /locus_tag="VGB68_18775"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0051348.1"
                     /translation="MSPRQQQIEKVFQAALTMGEAERTVFLAETCADEEMREEVEKLL
                     TGEMSENTLAAETTEDRELDAMLGRQIGVYRLVRELGRGGMGAVYLAERVDGAFSRQV
                     AVKLIKRGMDTDLVVRRFRNERQILATLNHPNITRLLDGGSTEKGLPYFVMEYVEGEQ
                     LYAYCDKRRLSIGERLRIFQKICEAIEEAHRHKIIHRDLKPSNILVKLDGTPKLLDFG
                     IAKLLDPEFGVTAMEPTATHMRMMTPQYASPEQISGEAVTPASDIYSLGVLLYELLTG
                     HRPYRVKSRAPYEIARAVREEEPTRPSEILTREDNFARSDTGTGEKPSLDIIFAARGA
                     TREALQYELAGNLDKIILKTLRKSASERYQSAAELSEDISNYLEKRPVRAQSFDLPEK
                     PAVQAEQATKNSIAILPFKLLVAERSDETGDEYLSIGLADALGSRLSGVKRLVVRPTS
                     SILRYADSADPFAAGRELNVEFIVDGNIRRSGERIRVTVQLLSIGENAARWAESFDEK
                     MTDVLEIEDLISERVVKILVSHLTGEEKNQLSKRATNNAEAYEAYLRGRHHWNQFTVE
                     GFAKALAFYRRAIELAPDYALPYAGIADYYNFLGIYAVRPFAETTEATKKAAQQAIAL
                     DPELAEGYAALGFAAQMGDFDWETAENYFRRAIELNPNYSIGRVWHAYHLGMCGRFDE
                     AFDQVNKALELDPFTPIVRQTLNWVLFYSRRYEEAIAGTYRLIEKEPHYGLAHLFLIG
                     VLARAGRYDEAIKVGHDAVALLGRSPHTLCWLTAAYAAAGRNDETRALIGEIENLSAT
                     HYVSPYLLAMIYANLGDAEKAFELLDKALEIRDGRLSWLAVDPHFDTVAADARYERLL
                     KAINYPSVRRQKAPGAIKDKEKSIAVLPLKMIGTMNTDDEYLGIGLTDALISRLSNVH
                     RFVVRPTSSILHFRENTDPFAAGGELNVDYVLDGNIRRSGERIRVSVQLLSVADNSTE
                     WAQSFDEKASDILEFEDLISERVVKSLVPHLTGEEQQKLARRATDKAEAYEAYLRGRF
                     NWNLHSEEGFARAIQFYRRAVELDPNYALAFAAMTEYYIFLGIHCVIPFAEGAKPARE
                     AAEKAVALDPSLAEGYAVLGFVEITYNWDWAAAEKHLRRAIELNPNSVTAHSWLNTVL
                     LQRGSFDAALDEINRVIELDPDSILSLHLQAWAFYHARRFDESIAVHRKMLENEPNYA
                     WGLQTLSWVLRQTGQYEEAVAASEKSVRLTGENPFYLTQSAAAYASAGNRAEAEKILA
                     RLREISATRFVSEYMLALVYCALGERDEAFENLEKSLQTRDGWLNWIGVDPQFDVLRG
                     DARYVDLLRRSGNPLAPPEEFKNISLDTGGARSIAVLPLKLFGTPTGAGEDEYLGIGL
                     TDALITRLSNVRRFVMRPTSSVLQYAQTEVDPFEAGQKLAVDYVLDGSVRRSGDRIRV
                     TVQLLSVSETATVWAESFDEKSSDVLELEDSISEKVARSLIPQLTGEERRQLEKRGTN
                     DPQAYEAYLRGRFHWNQFTPDALIKALESFQKAINLDPTYALAYVGLADFYTWANIYG
                     IVPSPVAHEKA"
     gene            4869..6557
                     /locus_tag="VGB68_18780"
     CDS             4869..6557
                     /locus_tag="VGB68_18780"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEY0051349.1"
                     /translation="MITSFNFRQIRSLFALAALVAFAAASFLLTPQSARMQSQISPRI
                     VFDRDKQVRVVNADGSADTMLAEGFDPVWSPDGTKIAYANGVEYNAEIYIMDADGENQ
                     IQLTQNWYSSYAPAFSPDGTKIAFVSNHEGDYRIYTINTDGTNQQRINFAGNFIGAHA
                     PVFSADGTKIVFVGTTVTETGTSRDEYYIANADGSAPVRLTFTGGFNRYQASVSPDGT
                     NILVSRWLDIYAVKTDGSGAMTNLTNSSPAETDADYSPDGSQIVFRRGGNVGLMIMNA
                     DGTNVTALNVLGYNPDWLPTAVSPTPTPTVTPTVTPTPSTDLNVQVSASAQNVNVGEI
                     VTFTVNVRNNGDAATNVKVFGAASIDGILNQVHYTSLNAPGGCTLVVGGSSVECQIGA
                     LAPNQSAVVTIGVRPNVAGSLIFNVGTSATEPDRNEADNAASAGVQVSTAPVCAANVT
                     SQVNIVRLPPIRNPRTNEYEQLIIARNTSNQNLHPRAMFVFDNLTSGVSVAPRSNPGT
                     TQCAAPIGSQYVTVNVGGNAWRPNQAAMVRVTFRNPQRRAVNYDLRVMAGANNP"
     gene            6773..9028
                     /locus_tag="VGB68_18785"
     CDS             6773..9028
                     /locus_tag="VGB68_18785"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0051350.1"
                     /translation="MNGSVLNVAAQTRRASNQQTPKSAAKCSGAWTGNVTYTRTQTTT
                     DSKTVKRVSNRGEDKREWEMRYNYKASVAVLEAPEKNGSNIGKGRIEHKFSSIDKTVA
                     REENSCDRGKTFQVMTGNFLTKTETTGNATNAEANVNVGVNADGTYTVSVGLPQIQGL
                     TTGKETSSFSGQCQPKKGKDLTLPEMPTSVDGNSLTSEGKDRVNPQDPNRLSGSYSKT
                     WQNVTETISWNLERCGAPLRITDLQFEDMKYPNWNDWQEIGEKTGTIDGNLVRIRAKI
                     LNESGETKYADVRFKETYKGDKWDGARPDAPLKDDVLSVRVEANSEETVELVWDSSGY
                     AWFDDGRPRLLQRIKAELEENSKKIGEMTENLKVAPKPLVLVHGLWSNWQAWESWQNI
                     LTTSHSYDWKAFPVGEKPEHGKMNTGGAFLSTDKTNSIGENAWELEKYVEYAQKDRNA
                     WHVDVVAHSMGGLISRYYISQVMKNNSEDGRPRVSHLVMLGTPNLGSPCADVMNVAIE
                     MLGKNVEAVRQLRVDQVANFNTVNKLRKGVKFSALAGNPLPTMCKEVVWNDGVVSVPS
                     AKWTIADTAESKSLHTDLTGTKDFSDFVKPRVAIGPKGNHNPEMPDMPNRAGQSDKNP
                     AGAYFMNASYKPRAETEDSIEQNAFKPDFAKSVKIGARQSVELEIPVGQALNFGLTFM
                     AASDISATLYDDKGAIAGKNLTKTPEAGSWFRSIYYDKPTTAGTWKLKLENASDRELE
                     AILATWKDAVK"
     gene            9204..9629
                     /locus_tag="VGB68_18790"
     CDS             9204..9629
                     /locus_tag="VGB68_18790"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HEY0051351.1"
                     /translation="MSYQLADNSNSINAQPSSFGLSPNAAALVSYIWVPVTSILVIAT
                     EKQNRMVRFHAWQSLFFGLGIFALTIVLSIVIGVLMLVAGAISPYLGIIISLGSLLVW
                     MILAAAILGVWIMCLVKAYKGEMYRLPVVGKYAENMSSK"
     gene            9874..10347
                     /locus_tag="VGB68_18795"
     CDS             9874..10347
                     /locus_tag="VGB68_18795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051352.1"
                     /translation="MKNQLNILLVLILLLGVSLACNRSEAKPGSTDLKNQTETRATKT
                     EDGGAATRLDSYSLRGLEFIYYKIPADLSREQLIETAQKLHEEEPKAQLILVDDDSQV
                     EDYVKYAKAVSGGDYDADFPKQWAEDHIVANVQKLLSGKWMVYESYGYKEIAELK"
     gene            10391..11197
                     /locus_tag="VGB68_18800"
     CDS             10391..11197
                     /locus_tag="VGB68_18800"
                     /inference="COORDINATES: protein motif:HMM:NF042732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6683 family protein"
                     /protein_id="HEY0051353.1"
                     /translation="MKKIIYLFAFIFTVFAFSAAPQNASAQYNSNYGSSIWANSNMNL
                     LFQKRWEAAQIRAAGKTQLADALEGRRSGGGNPTSSQSAPAENILRRVPLTETSFKSN
                     PEPIMPAVLGASLVDAKPEERQALVKVSRELLKNYEEMLVADKETRVRNNAAGAAAFA
                     LVTSRSVLTDGSRELSEKQTEALLQDINALLASSESFKKMSDVDKQKMYETFVITGGL
                     AMKLYREGTEKGDAETIAKGRDLAKTILSQFFRRSLNEISFTEDGVQFGF"
     gene            11245..11622
                     /locus_tag="VGB68_18805"
     CDS             11245..11622
                     /locus_tag="VGB68_18805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051354.1"
                     /translation="MKFARYTFLIAGIYGLIVLAPQYFLEAKNGLDFPPAITHPEYYY
                     GFTGVALAWQIAFLIISRAPSRYRPMMIPAVLEKLSFGAAAIVLFWQNRLPLMLLGAG
                     ILDLILGALFVVAFLKTKENRQT"
     gene            complement(11996..>16648)
                     /locus_tag="VGB68_18810"
     CDS             complement(11996..>16648)
                     /locus_tag="VGB68_18810"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0051355.1"
                     /translation="LQLFQQICAAVYYAHQNLIIHRDIKPSNILVTEGGVPKLLDFGI
                     AKLLNPDLIHETQEPTATAMRMMTPEYASPEQVSGNFVTSATDQYSLGVLLYEIVTGH
                     RPYLLENRSPLEIARVICEKSPATPSVIVGSSQQLVNSNKFSLEPVTLETIFRNRKTT
                     LDTLKIELESGLDALLLQSLHKEPHRRYSSVEQLSEDIKRFLLGQPIKAKPSFQMHQS
                     ETSETTGGTKQKTLAVLPFKLLNIIANSGAHDTGDEFLGIGLADALITRLGNMRRFSV
                     RPTGTVLQYATAEEDSLTIGKRLGVAYVLDGRILKVRDKIRVTVQLISIKDGASIWAE
                     QFDENVADVLALQDSLSTKVAAALIPQISTEELEQIAFVGTKDEAAHEKYLRGRYCWH
                     KFTEESLAKSIVYFYEAIALDPNYARAYSGVADYHTWLGIVGVLPPKECFGAARQAAE
                     KAVELDPHLAEAHASLAFSKWAFNWDFAEGEKLFRHALELNPNYFQTHEWLALFYSSL
                     GRYDEALNEINIAQRLNPISASIPIIASQILFKARRFEESLAQNNRAIQLEPSNSHVL
                     QSYGWILPEIERADEAVEFCRRAVEISERAPSALAAYGYALARKGKREEAEQILREML
                     EARVHRYVSQFYAALVQAALGNKKDALRSLRQAEKDKDYRMFWLETDARFDSLRGEKE
                     FQEIVESLAKLKNVNPIVAHFTFEKSVEPTDTDGAIHKTAVTKRFGETFRSEAPKSKT
                     PPREVAEKDARRSGWKPVAAALAVLLLIVGAGLGFSYYKYGTWTFSAPTVTGSGGEVQ
                     TFVPGKVIPLTSSSAVEKNPKLSPDKSQVLFVSNREGNSEIYVMNLDGTDLRRVTNNK
                     TEETSANWSPDGKRIVFESYSQIGVESDIWIMDADGANQQNLTNFPGLDMRPIFAPDG
                     SKIAFGSNRSLNDRREANIWIMNADGSNPKQLTDNTAFESDPNWSPDSKKIAYTKAMT
                     GSVFDIWMMNADGSEQTNVSNTDKSDEALPVFSPDGNRFVYSTNFESLSPRYDLWVMD
                     TSGENRQRVTINPANDLEAVWLSDTKLVFQSNREANYKIYQTDLNDSEVPAERRPKAV
                     NSIAILPFYLVNLNPADEHLSLGIAEAITSRLGQIKQLSVRPVTASSKKAASAEEAIN
                     IGRELAANYVLFTQLEKVNDNLQVTAALQNTEKGELVWSEKFSIHEKELSQLQDLISE
                     RVVQNLRLELSLNERETLNRKTTLNEEAYQFYLAGRYNLAKRKTENFKIALVNFEQAI
                     ALDPKFAQAYAGLADAYALMNLYPPAPPDGYRKARENAEKALNLNDQLADANATLGLV
                     EFYGERRFGEAEKYLRRAIELNPSYSQARHWLALVLSGSGKSDEALRQIEIAERLDPQ
                     SLFAVAAHGNILLYARQYGLAAEQYRKVLRRDAGFINAYMGLRRALELQGEFDEAMSV
                     LGQERIYHAGSNEFHLRLSLAETYAAGNKKAEAEKAIAEAFSLPDADKLQKTSAYHIS
                     TIYALLGNAGETLKWLRLAEAEKRSEINFIAVSPFFDNVRENAQFGEFRRRVLPSG"
BASE COUNT         3724 a         4618 c         4575 g         3731 t
ORIGIN      
        1 cggctttttc gtgcgccacc ggcgacggca caatgccata aatgttcgcc cacgtataaa
       61 aatccgccag accgacatat gccagagcgt atgtcgggtc gagattaatg gctttttgaa
      121 acgattcgag agctttaatc aaagcgtcgg gcgtaaattg attccagtga aaacgcccgc
      181 gcaggtaagc ttcgtaagcc tgcgggtcgt tcgttccgcg tttttccagt tggcggcgct
      241 cttcgcccgt cagttgggga atcagcgagc gggcgacttt ttccgaaatc gaatcttcca
      301 gttccagaac gtccgacgat ttttcgtcaa agctttccgc ccagaccgtg gcggtctcgc
      361 tgacgcttaa aagctggacg gtcacacgga tgcggtcgcc gctgcgccgc acgctgccgt
      421 ccagaacata atcgaccgcc agtttttgtc ccgcctcgaa cgggtcgact tccgtttgcg
      481 cgtattgcag aacggagctg gtcgggcgca tcacaaaacg ccggacgttt gaaagccgcg
      541 taatcagcgc gtcggtcaag ccgatgccga gatattcgtc ttcgcccgcg ccggtcggcg
      601 tgccgaataa tttcaaaggc agaacggcaa tcgaccttgc gccgcccgta tccaaactaa
      661 tatttttaaa ttcttcgggc ggagctaaag gatttcccga acggcgcagc aaatcgacat
      721 aacgcgcgtc gccgcgcaaa acgtcgaact gcgggtcgac gccaatccag ttgagccatc
      781 cgtcgcgcgt ctgaagcgat ttttcgagat tctcgaaagc ctcgtcccgt tcgccgagcg
      841 cgcagtaaac cagcgcgagc atatattcgg aaacaaaccg cgtcgcggaa atttccctga
      901 gccgcgcgag aattttttcg gcttcggcgc gattgcccgc gctggcgtaa gcggcagccg
      961 actgcgtcaa ataaaaagga ttttcgcccg tcagccgaac ggatttttcc gaagcggcga
     1021 cggcttcttc gtattgcccg gtttgccgta aaacccagct taaagtctgc aagccccagg
     1081 cataattcgg ctcgttttcg agcattttcc ggtgcacggc gattgattcg tcaaagcggc
     1141 gcgcgtgata gaaagcccac gcctgaaggt gcaggctcaa aatcgaatcg gggtcgagtt
     1201 cgatgacgcg atttatctcg tcgagcgcgg cgtcaaagct cccgcgctgc aaaagaaccg
     1261 tgttcagcca gctatgcgcc gtgaccgaat tcgggttcag ctcgatggcg cggcgcagat
     1321 gtttttccgc cgccgcccaa tcccagttat aggtaatctc gacaaagccc agaacggcgt
     1381 agccttcggc gagcgacggg tcgagcgcga cggctttttc cgccgcttct ctggcgggct
     1441 tcgctccttc ggcaaacgga ataacgcagt gaataccgag aaaaatataa tattcggtca
     1501 tcgccgcaaa ggcgagcgcg taattcgggt cgagctcgac ggcgcgccgg taaaactgaa
     1561 tcgcgcgggc aaagccttct tcgctgtgca gattccaatt gaaacgaccg cgcagataag
     1621 cttcataagc ttcggctttg tcggtcgcgc ggcgggcgag tttttgctgt tcttcgcccg
     1681 ttaaatgcgg aacgagcgat ttgacgacgc gctcggaaat caaatcttca aattcgagaa
     1741 tgtcggaagc tttttcgtca aagctctgcg cccattcggt cgaattatcg gcgacgctca
     1801 aaagctggac gctgacgcgg atgcgctcgc cgctgcgccg gatattgccg tccagaacgt
     1861 aatcgacgtt aagctcgccg cccgccgcaa acgggtcggt gttttccctg aaatgaagaa
     1921 tcgagctggt cgggcgaacg acaaaacgat gaacgtttga aagccgcgaa atcagcgcgt
     1981 cggtcagacc gatgccgaga tattcgtcgt ccgtattcat tgtgccgatc attttcagcg
     2041 gcagcacggc gattgatttt tctttatctt ttatcgcgcc cggtgctttt tgccgcctga
     2101 cggaaggata atttatcgct ttcaataatc gctcgtagcg cgcgtcggcg gcgaccgtgt
     2161 caaaatgcgg atcgaccgcc agccagctca gcctgccgtc gcggatttcg agcgctttat
     2221 cgagcaattc aaaggctttt tccgcgtcgc ccagattggc gtaaatcatc gccaaaagat
     2281 aaggcgaaac atagtgcgtc gcggataaat tctcgatttc gccgattaag gcgcgcgtct
     2341 cgtcgtttcg cccagccgcc gcgtacgccg cagtgagcca gcacagggtg tgcgggcttc
     2401 tgccgagcag cgcgacggcg tcgtgaccga cctttatcgc ttcgtcgtat ctgccggcgc
     2461 gcgccagcac gccgatcaaa aataaatgcg ccaacccgta atgcggctct ttttcaatca
     2521 gccggtaagt tccggcaatc gcttcttcat agcggcgcga gtaaaacaaa acccagttca
     2581 gggtctgccg gacgataggc gtgaaggggt cgagctcgag cgctttattg acctggtcga
     2641 aagcctcgtc aaagcgcccg cacatcccga gatgataagc gtgccagacg cgcccgattg
     2701 aataattcgg gttcagctcg attgcccggc gaaaataatt ttcggcggtt tcccaatcga
     2761 aatcgcccat ctgcgcggca aatccgagcg cggcgtagcc ttccgccagt tccgggtcga
     2821 gcgcgatcgc ctgctgcgcc gcttttttcg tcgcctcggt cgtttcggcg aaagggcgga
     2881 cggcgtaaat gcctaaaaaa ttgtaataat cggcgatgcc ggcgtaagga agcgcgtaat
     2941 cgggcgcgag ctcgatggcg cgccggtaga aagccagagc tttggcgaag ccctcgaccg
     3001 taaactgatt ccagtgatgg cgcccgcgca gataagcttc gtaggcttcg gcgttgttcg
     3061 tggcgcgctt cgacagttga tttttttcct cgcccgtcag atgcgaaacc aggattttga
     3121 cgacgcgctc ggaaatcaaa tcttcaattt ccagaacgtc agtcattttt tcgtcaaaac
     3181 tttcagccca gcgcgccgcg ttttcgccga tgctcaaaag ctggacggtg acgcggatgc
     3241 gctcgccgct gcgccggatg ttgccgtcga cgataaattc gacattcagc tcgcgcccag
     3301 ccgcaaacgg gtcggcgctg tcggcgtagc gcagaatcga gctggtcggg cgcaccacca
     3361 gacgcttaac gccgctcaga cgcgagccga gcgcgtccgc cagaccgatg ctcagatatt
     3421 cgtcgcccgt ctcgtcgctc cgctccgcta cgagcagttt aaacggcaga atggcaatcg
     3481 agtttttggt cgcctgttcg gcttggacgg cgggcttttc cggcaaatcg aacgactgcg
     3541 cgcggacggg acgtttttcc agataattgg aaatgtcctc gctcaattcg gcggctgatt
     3601 gataacgctc cgacgcgctt ttgcgcagcg ttttcaaaat gattttgtcg agattgcccg
     3661 ccagctcgta ctgcaaagcc tcgcgtgtgg cgccgcgcgc cgcaaaaatt atatcgagcg
     3721 acggcttttc gcccgttccg gtatcgctgc gcgcaaagtt gtcttcgcgc gtgaggattt
     3781 cgctcggtcg cgtcggttct tcttcgcgga cggcgcgggc gatttcatac ggcgcgcggc
     3841 ttttgacgcg atacgggcga tgtccggtca aaagctcgta cagcagaacg cccaggctgt
     3901 aaatgtcgga cgcgggcgtg accgcctcgc cggaaatctg ttcgggcgaa gcgtattgcg
     3961 gcgtcatcat tcgcatatgc gtcgccgtcg gctccatcgc cgtcacgccg aattccgggt
     4021 cgagcaattt ggcgatgccg aaatcgagca gcttcggcgt gccgtcgagc ttgaccagaa
     4081 tattcgacgg cttcaaatcg cggtgaataa tcttgtgacg atgcgcttct tcaatcgcct
     4141 cgcagatttt ttgaaaaata cgcagtcttt cgccgatgct caggcggcgt ttatcgcaat
     4201 aagcgtaaag ctgctcgccc tcgacgtatt ccatcacgaa atacggcaag cctttttcgg
     4261 tcgagccgcc gtcgagcagg cgcgtgatgt tcggatgatt tagggtggct aagatttgcc
     4321 gctcgttgcg aaagcggcgc acgaccaaat ccgtatccat cccgcgcttg ataagcttga
     4381 cggcgacctg tcgcgagaaa gcgccgtcga ctctttccgc cagataaacc gcgcccattc
     4441 cgccgcgccc gagctcgcgc accagccggt aaacgccgat ttgccgtccg agcatcgcgt
     4501 cgagctcgcg gtcttcggtc gtttcggcgg caagcgtatt ttccgacatt tcgccggtca
     4561 acagtttttc cacttcctcg cgcatctctt catccgcgca ggtttccgcc agaaacacgg
     4621 tgcgctccgc ctcgcccatc gtcagcgcgg cttgaaaaac tttttcgatt tgttgctgtc
     4681 tgggggacat cgttttttca gttaaaagat tgacgcaagt atagcgaaat tagccggagc
     4741 cgacaatttc ctgtcgaaat tattcgcccg cctgatactt ttttcttccg cttgacgcaa
     4801 ttacagacgg cgcggcgatc aagcgccgca aattttattt tattttttaa tcaacaggag
     4861 aaaccgcaat gattacatct tttaattttc gacaaattcg ctcgctcttt gctctggcgg
     4921 cgctggtcgc ttttgccgcc gcgtcgtttt tgctgacgcc gcaaagcgcg cggatgcaaa
     4981 gccaaatcag cccgcgcatc gtgtttgacc gcgacaagca agtgcgcgtc gtcaatgccg
     5041 acggctcggc ggacacgatg cttgccgagg gcttcgaccc ggtctggtcg ccggacggca
     5101 cgaaaatcgc ctacgctaac ggcgtcgaat acaacgccga aatctatatc atggacgctg
     5161 atggcgaaaa tcaaatccaa ctgacgcaaa actggtacag cagctacgcg ccggcttttt
     5221 cgcccgacgg cacgaaaatc gcttttgtca gcaatcacga aggcgattac cgcatctaca
     5281 cgataaacac ggacggcacg aaccagcagc gcatcaattt cgcgggcaat tttatcggcg
     5341 cacacgcgcc cgtgttttcg gctgacggca cgaaaatcgt cttcgtcggc acgaccgtca
     5401 ccgaaaccgg cacgtcgaga gacgaatatt atatcgccaa cgccgacggc tcggcgcccg
     5461 ttcgtctgac ttttaccggc ggctttaacc gctatcaggc atccgtttcg ccggacggca
     5521 caaacatttt agtctctcgc tggctggaca tctacgcggt caaaacggac ggcagcggcg
     5581 cgatgacgaa cctgaccaat tcgagccccg ccgaaaccga cgcggattat tcgcccgacg
     5641 gctcgcaaat cgtttttcgc cgcggcggca atgtcggctt aatgattatg aacgccgacg
     5701 gcacaaacgt cacggctttg aatgttctcg gctacaaccc cgattggctg ccgaccgccg
     5761 tcagcccgac cccgacgccg accgtcacgc cgacggtgac tccgacgcct tcgaccgatt
     5821 tgaatgtgca ggtcagcgcc tcggcacaga acgttaatgt cggcgagatt gtgactttta
     5881 ccgtcaacgt gcgcaacaac ggcgacgcgg cgacaaacgt gaaagttttc ggcgcggcat
     5941 cgattgacgg catactcaat caggttcact acacttcgct gaacgcgccg ggcggctgca
     6001 cgctcgtcgt cggaggctca agcgtcgaat gccaaatcgg agcgctcgcg ccgaatcaat
     6061 cggcagtcgt caccatcggt gtccgtccga acgtcgcggg cagcctgatt ttcaatgtcg
     6121 gcaccagcgc gaccgagcct gaccgcaacg aagcggacaa cgcggcttcc gccggtgttc
     6181 aggtcagcac ggctccggtc tgcgccgcca acgtcacctc gcaggtgaac atcgtccggc
     6241 tgccgccgat tcgtaatccg cgcaccaatg aatacgagca gctgattatc gcgcgaaata
     6301 cctcgaatca aaatctgcac ccgcgggcaa tgtttgtttt cgataatctg accagcggag
     6361 tctcggtcgc gccgcgctcg aatccgggca cgacgcaatg cgccgcgccg atcggcagcc
     6421 aatacgtgac ggtgaacgtc ggcggcaacg cgtggcgacc gaatcaggcg gcgatggttc
     6481 gcgtgacttt cagaaacccg cagcgccgcg ctgtcaatta cgatttgcgc gtgatggcgg
     6541 gcgcaaataa cccgtaaatt tatcgttaaa tctccagacg ataaacgaca ggcggcgcgc
     6601 gggaaaattt tccttcgcgc cgcctgatac ttttcgccgc cggtttacgc catacagtca
     6661 gaaacaaatt tgccgccgcc ggcgcgggtt gctttcaagg gaaatcatgc aaaaaatcgt
     6721 atcaaacgga cttttcaaaa aacttttttt aactgttttg tgcctcggcg ttttgaacgg
     6781 cagtgttctt aacgtggcgg cgcagacgcg gcgcgcttcg aatcaacaga caccgaaatc
     6841 cgcagcgaaa tgctcgggcg cgtggacggg caacgtcacc tacacgcgaa cgcagacgac
     6901 gaccgacagc aaaaccgtca agcgcgtctc gaatcgcggc gaggacaagc gcgaatggga
     6961 gatgcgctac aactacaaag cgtcggtcgc cgtcctcgaa gcgcccgaaa aaaacggctc
     7021 gaacatcggc aaagggcgca tcgagcataa gttttcatca atcgacaaaa ccgtcgccag
     7081 agaagaaaat tcgtgcgacc gcggcaagac ttttcaggta atgaccggaa atttcctgac
     7141 gaaaaccgaa acgaccggaa atgcgaccaa cgccgaggcg aatgttaatg tcggcgtcaa
     7201 tgcggacggc acatacacgg tcagcgtcgg cttgccgcag attcaagggc tgacgaccgg
     7261 aaaagaaact tccagcttca gcgggcagtg ccagccgaaa aaaggcaaag atttgacgct
     7321 gccggaaatg ccgacgagcg tcgacggcaa ctccttaacc agcgaaggca aagaccgcgt
     7381 caatccgcaa gacccgaatc gtctttcggg aagctattcg aaaacgtggc agaacgtcac
     7441 ggaaacgatt tcgtggaatc tggaaagatg cggcgcgccg ctgcggatta ccgatttgca
     7501 gtttgaagat atgaagtatc cgaactggaa cgactggcag gaaatcggcg aaaaaacggg
     7561 aacgattgac ggcaatctgg tgcgcatcag agcgaaaatt cttaatgaat cgggcgagac
     7621 gaaatacgcc gacgtgcgtt ttaaggaaac ctacaaaggc gataaatggg acggcgcgcg
     7681 accggacgcg ccgctgaaag acgacgtcct ctcggtgcgc gtcgaggcga actcggaaga
     7741 aacggtcgaa ctggtctggg attcgtccgg ctacgcgtgg tttgacgacg gtcgcccgcg
     7801 gctcctgcag cgcatcaaag ccgagctcga agaaaacagc aaaaaaatcg gcgagatgac
     7861 cgaaaatctg aaagtcgcgc cgaaaccgct ggttctggtt cacgggctct ggagcaactg
     7921 gcaggcttgg gagtcgtggc agaatatttt gacgacttcg cactcttacg attggaaagc
     7981 gtttccggtc ggcgaaaagc cggaacacgg caaaatgaac acgggcggcg cgtttctttc
     8041 gaccgataaa acaaattcca tcggcgaaaa tgcgtgggag ctggaaaaat acgtcgagta
     8101 cgcgcagaaa gaccgcaacg cgtggcacgt cgacgtggtc gcgcattcga tgggcggttt
     8161 gatttcgcgt tattacatca gccaggtgat gaagaataat tccgaggacg gtcgcccgcg
     8221 cgtctcgcat ctggtgatgc tcggcacgcc gaatttgggc agcccttgcg ccgacgtgat
     8281 gaacgtcgcc attgaaatgc tcggaaaaaa cgtcgaagcc gtccgtcaat tgcgggtcga
     8341 tcaggtcgcc aatttcaata cggtcaataa acttcgcaaa ggcgtgaaat tttccgcgct
     8401 tgccgggaat ccgctgccga cgatgtgcaa ggaagtcgtc tggaacgacg gcgtcgtatc
     8461 ggtgccgtcg gcaaaatgga caatcgccga caccgccgaa tcgaaaagcc ttcacacgga
     8521 tttgaccgga acaaaggatt tttcggattt cgtgaaaccg cgcgtcgcca tcggaccgaa
     8581 aggcaaccac aatcccgaaa tgcccgatat gccgaatcga gcgggacaat cggacaaaaa
     8641 cccggcaggc gcgtatttta tgaacgcgtc ttacaaaccg cgggctgaaa ccgaagattc
     8701 gatcgaacaa aacgctttca aaccggattt tgcaaagtcc gtgaaaatcg gcgctcgaca
     8761 atcggtcgag ctcgaaatcc cggtcggaca ggctcttaat ttcggtctga cgtttatggc
     8821 ggcgagcgat atttcggcga cgctctacga cgacaaaggc gcaattgccg gtaagaatct
     8881 gacgaaaacg cccgaagccg gctcgtggtt tcgctcgatt tattatgaca agccgaccac
     8941 ggctggaacg tggaaactga aactggaaaa cgcttcagac cgcgagctgg aagccattct
     9001 ggcgacgtgg aaagatgccg tgaaataaag aaaaagcaat ttgcggattg cccgcaaagc
     9061 tcgccgcggc gtttcttatc cgattcgtcg cggcgagctt ttctttttcc ggaaaacatt
     9121 tttccgcctg atacttttgc ccgcgattct ccgctttacc aattagaaaa aacttatatc
     9181 tatttatcgg caaaggattt tttatgtcat atcaactcgc agacaattcg aattcaatca
     9241 acgctcagcc gtcgagtttc gggctttcgc cgaacgcggc tgcgctggtc agctacatct
     9301 gggttcccgt cacgagcatt ctcgtcatcg ccaccgaaaa acagaaccga atggtgcgtt
     9361 ttcacgcctg gcaatcgctt tttttcgggc tcggcatttt cgccctgacg attgttttaa
     9421 gcatcgtcat cggcgtcctg atgctggttg ccggcgcgat ttcgccgtat ctcggaatca
     9481 tcatctcgct cggctcgctg ctcgtctgga tgattctggc agcggcgatt ctgggcgtct
     9541 ggattatgtg tctggtcaaa gcttataaag gcgaaatgta ccggcttccg gtcgtcggca
     9601 aatacgccga aaatatgagc tccaaataag catccggctt ttaccggttc ccgctccgtt
     9661 aatatattcg aggcgtttca gtgtttgctg aaacgcctct tttttagaag gctgaacgcc
     9721 gttcggattc gaaatatcag gcgataaact tcaattccga actgtcttcc gcctgccgac
     9781 tgtcgcccct gttcttccga ctttccctga tacttttccg gctcgatttg cgctttaaca
     9841 agtgaccgac aacttttaca ggagaacatc ccaatgaaaa accaacttaa tattttactc
     9901 gtcttaattt tacttttagg tgtcagcctg gcgtgcaatc gctcggaagc caaaccgggc
     9961 agcacggact taaaaaatca aaccgaaacc cgcgcgacga aaaccgaaga cggcggcgcg
    10021 gcgacgcgtc tcgacagcta cagcttgcgc ggtctggaat ttatatacta caaaattccg
    10081 gctgatttaa gccgcgagca gttgatcgaa acggcgcaga agctgcacga agaagagccg
    10141 aaagcgcagt tgattctggt tgacgacgac tcgcaggtcg aagattacgt caaatacgcc
    10201 aaagccgtca gcggcggcga ttacgacgcg gattttccga aacagtgggc ggaagaccac
    10261 atcgttgcca atgtccagaa gcttttgagc ggcaaatgga tggtctatga aagctacggc
    10321 tacaaggaaa tcgccgaatt gaaataattt aaaactgaag acgcgattaa atagaaaagg
    10381 agctcgcgct atgaaaaaaa taatttatct cttcgcattt atttttacgg ttttcgcttt
    10441 ttccgccgcg ccgcaaaacg cgagcgcgca gtataactcc aattacggca gcagcatctg
    10501 ggctaacagc aatatgaacc tgcttttcca gaagcgctgg gaagcggcgc aaattcgcgc
    10561 ggcgggcaaa actcagctcg ccgacgccct ggaaggcaga cgctccggcg gcggaaatcc
    10621 aacgagctct caaagcgcgc cggcggaaaa cattctgcgc cgcgtccctt taacggaaac
    10681 ttcgtttaaa tcgaatccgg agccgattat gcccgccgtt ctcggcgcgt cgctggtcga
    10741 tgccaagccg gaagagaggc aggcgctcgt caaagtttcg cgcgaactgc tcaaaaatta
    10801 cgaggagatg ctcgtcgccg acaaggaaac gcgcgtcaga aacaacgcgg cgggagcggc
    10861 tgcttttgcg ctcgtgacga gccgctcggt cttgacggac ggcagcagag agctttccga
    10921 aaaacagacc gaagcccttt tgcaggatat caacgcgctg ctcgcctcga gcgaaagctt
    10981 caaaaaaatg tccgacgtcg ataaacaaaa aatgtacgaa acgtttgtca tcaccggcgg
    11041 tctggcgatg aagctttacc gcgaaggcac ggaaaaaggc gatgccgaaa caatcgccaa
    11101 aggcagggat ttggcgaaga cgattttgtc gcagttcttt agacgctcgc tgaatgaaat
    11161 cagttttact gaagacggcg tgcaattcgg cttttaatgt agaggcgcac cgaattttcg
    11221 cgcgcgtctt tcgaccggag aaaaatgaaa tttgcccgat acacgtttct gattgccgga
    11281 atttacggct taatcgtact cgcgccgcag tattttctcg aagcgaaaaa cggtctggat
    11341 tttccgcccg cgataacgca tcccgaatat tattacggtt ttacgggcgt cgcgctggcg
    11401 tggcaaatcg cttttctgat tatctcgcgc gcgccgtcgc gttatcgccc gatgatgatt
    11461 ccggcggtgc ttgaaaaact cagtttcggc gctgccgcca tcgttttgtt ctggcaaaac
    11521 cgtctgccct tgatgcttct cggcgcggga attttggatt taattctcgg cgcgcttttc
    11581 gtggtcgctt ttcttaaaac gaaagaaaat cgccaaacgt aaagccgcgc cggaaacgat
    11641 tcggaaaaca aggcgaatcc ggaatcgccg cgttttccgg caaagcgcgc cgttcgtccg
    11701 gttttatcac gctctaaccg gcgacggacg gagtaaagca tttatttgca agattcggtt
    11761 atctccgcaa aatatgcttt actccggtct cgcgcgatta aaaggaaatt cagcgcgcgg
    11821 agcgcaaaaa gaaatagccc gccgacaaac cgccgccgag ccggataaga aatagattcg
    11881 aaatctgaaa tctgaaataa caaaatcgca attttcgctt gcctgtcggc gtccgcccgt
    11941 gtttatctgc gggcgattct tttccaaagc gttccttgcg tttttgcggc gaaaattaac
    12001 cggatggcaa aacgcgccgc cggaattcgc cgaactgggc gttttcacgc acgttgtcga
    12061 agaaagggct gacggctata aaatttatct cgctgcgttt ttcggcttcg gcgaggcgca
    12121 gccattttaa ggtttcgccc gcattgccga gcagcgcgta aatggtcgag atatgatacg
    12181 ccgacgtttt ttgcagttta tcggcgtcgg gcagtgaaaa ggcttcggca atcgcttttt
    12241 cggcttcggc ttttttattt cccgccgcgt aagtttccgc caggctcagg cgcaggtgaa
    12301 attcgttgct gcccgcgtga tagattcttt cctgaccgag cacgctcatc gcctcgtcaa
    12361 actcgccctg aagctcgagc gcgcggcgca agcccatata agcattgata aatccggcat
    12421 cgcggcgcaa aactttgcgg tattgctcgg cggcgagccc gtattgacgc gcgtataaaa
    12481 ggatattgcc gtgagcggcg acggcaaaca gcgactgcgg gtcgaggcgt tcggcgattt
    12541 ctatctgccg caaagcttcg tcggattttc cgctgccgct caaaaccagc gccagccagt
    12601 gacgcgcctg cgaatagctc ggattcagct cgatggcgcg ccgcagatat ttttccgcct
    12661 cgccgaaacg gcgctcgccg taaaattcga cgagcccgag cgtcgcgttg gcgtcggcta
    12721 actggtcgtt caaattcaac gccttttcgg cattctcgcg cgcttttcga taaccgtccg
    12781 gcggcgcggg cggataaaga ttcatcagcg cgtaagcgtc cgccagaccg gcataagcct
    12841 gcgcaaattt cgggtcgagc gcgattgcct gttcgaaatt aaccagcgcg attttgaaat
    12901 tttcggtttt gcgcttcgcc agattgtagc gaccggcgag ataaaattga taagcttcct
    12961 cgttgagcgt ggttttccgg ttgagcgttt cgcgctcgtt caggctcagt tcgaggcgca
    13021 gattctgcac gacgcgctcg gaaatcaaat cctgcagttg agaaagctct ttttcgtgga
    13081 tggaaaattt ttccgaccag accagctcgc ccttttccgt gttttgcaaa gcggcggtga
    13141 cctgcaaatt atcgttcacc ttttcaagct gcgtgaacaa aacgtaattg gcggctaatt
    13201 cgcgcccgat gtttatcgct tcttcggcgg aggcggcttt tttgctgctg gcggtcaccg
    13261 ggcgcacgga taattgtttg atttgaccga gcctcgacgt gatggcttcg gcgattccga
    13321 ggctcaagtg ctcgtccgcc ggattcagat tcaccagata aaacggcaga atcgcaatcg
    13381 aattgacggc tttcggtcgc ctttccgccg gaacctcgct gtcgttcaaa tcggtttgat
    13441 aaattttgta attagcttcg cggttgctct gaaaaacgag ctttgtatcc gaaagccaga
    13501 ccgcttccaa atcattcgcc ggatttatcg tcacgcgctg gcgattttcg ccgctcgtat
    13561 ccatcaccca caaatcgtag cgcgggctga ggctttcaaa gttcgtcgag tagacaaaac
    13621 gatttccgtc gggcgaaaaa acgggcaggg cttcgtcgga tttatcggtg ttgctgacgt
    13681 tcgtctgttc cgagccgtcg gcgttcatca tccagatgtc gaaaacgctg ccggtcatcg
    13741 cctttgtata agcgattttt ttgctgtcgg gcgaccagtt cgggtcggat tcaaacgccg
    13801 tgttgtcggt cagttgtttc ggattcgagc cgtcggcgtt cattatccaa atgttcgcct
    13861 cgcggcggtc gtttaaactt cggttgctgc cgaacgcaat cttcgagccg tcgggcgcaa
    13921 aaatcgggcg catatccaga ccgggaaaat tcgtcagatt ttgctggttc gcgccgtcgg
    13981 cgtccattat ccagatatcg ctctcgacgc cgatttgcga atagctttca aagacgatgc
    14041 gcttgccgtc cggcgaccag ttggcgctgg tttcctcggt tttgttgttc gtcacgcgcc
    14101 gcaaatcggt gccgtccaga ttcatcacgt aaatctcgct gttgccttcg cggttgctga
    14161 cgaacaaaac ctgcgatttg tcgggcgaaa gcttcggatt tttttcgacc gccgaagaag
    14221 acgtcagcgg aatgactttg ccgggaacaa aagtctgcac ttcgccgccg ctgcccgtga
    14281 cggtcggcgc ggaaaacgtc caggtgccgt atttataata cgaaaaaccc aaaccggcgc
    14341 cgacaatcag gagcaaaacg gcgagcgcgg cggcaaccgg tttccagccg gaacggcgcg
    14401 cgtccttttc ggcgacttcg cgcggcggcg ttttgctttt cggagcttcg cttctgaaag
    14461 tctcgccgaa tcttttcgtc accgccgttt tgtgtatcgc gccgtcggtg tcggtcggct
    14521 cgacgctttt ctcaaaagtg aaatgcgcga caatcggatt gacgtttttc agcttcgcca
    14581 gagattcgac aatttcctga aactctttct cgccgcgaag cgaatcgaaa cgcgcgtcgg
    14641 tttcgagcca gaacattcga taatctttat ctttttcggc ttgtctgagg ctgcgcagcg
    14701 cgtctttttt attgcccaga gccgcctgca ccaacgccgc gtaaaactgc gagacgtagc
    14761 gatgaactct ggcttcgagc atctcgcgca aaatttgttc ggcttcttcg cgtttgccct
    14821 tgcgcgccag cgcgtagccg taggcggcga gcgccgaagg cgcgcgttcg gaaatctcga
    14881 cggcgcggcg gcaaaactcg acggcttcgt cggcgcgctc gatttccggc agaatccagc
    14941 cgtagctttg caaaacgtga ctgttcgaag gctcaagctg aatggcgcga ttgttttgcg
    15001 ccagagattc ttcaaagcgc cgcgctttaa agagaatctg cgaggcgatt atcggaatcg
    15061 aagcggaaat cgggttgagc ctttgcgcga tattgatttc gttcagggct tcgtcgtatc
    15121 gaccgagaga gctgtaaaaa agcgcgagcc attcgtgcgt ttgaaaataa ttcggattca
    15181 gctccagagc gtggcggaaa agtttttcgc cttcggcaaa atcccagttg aaagcccatt
    15241 tggaaaacgc cagcgacgcg tgcgcttccg ccagatgcgg gtcgagctcg acggcttttt
    15301 ccgccgcctg tcgcgccgcg ccgaaacatt ccttcggcgg cagaacgccg acaattccga
    15361 gccacgtatg ataatcggcg acgcccgagt aggcgcgcgc gtaattcggg tcgagcgcga
    15421 ttgcctcgta aaaatagacg attgacttgg cgagcgattc ttccgtaaat ttgtgccagc
    15481 agtagcgccc gcgcaaatat ttttcgtgcg cggcttcgtc ttttgtgccg acaaaagcaa
    15541 tctgttccag ctcctcggtc gaaatctgcg gaatcaaagc ggcggcgact ttcgtcgaaa
    15601 gcgaatcctg caaagccagc acgtcggcga cgttttcgtc aaactgttcc gcccagattg
    15661 atgcgccgtc cttgatgctg attaactgaa ccgtgacgcg gattttatcg cggactttta
    15721 aaattcgacc gtccagaaca taagccacgc ccagccgctt gccgatggtc agcgaatctt
    15781 cttcggcggt ggcgtattgc agaaccgtgc ccgtcgggcg cacggaaaaa cggcgcatat
    15841 tgccgagccg cgtaatcaga gcgtccgcca gaccgatgcc taaaaattcg tcgcccgtat
    15901 cgtgcgcgcc gctgttggcg atgatgttca gcagcttgaa cggcaaaacg gcgagcgttt
    15961 tctgtttcgt tccgccggtc gtctcgctcg tctcgctctg atgcatttga aacgaaggtt
    16021 tcgcttttat cggctgaccc agcagaaagc gtttgatgtc ttcggaaagc tgttcgaccg
    16081 acgagtagcg tcgatgcggc tctttgtgca gagattgcag aagaagcgcg tccaaaccgc
    16141 tttcgagctc gatttttaaa gtgtcgagcg tcgttttgcg attgcggaaa atggtttcca
    16201 gcgtcaccgg ctcgagcgaa aatttattcg agttgaccag ctgctggctg cttccgacaa
    16261 tcacgctcgg cgtcgcgggc gatttttcac agatgactct ggcgatttcc agcggcgagc
    16321 ggttttccag caggtaaggt cgatgcccgg tcacaatctc gtaaagcaaa acgccgaggc
    16381 tgtactggtc ggtcgccgac gtgacgaaat ttccgctgac ctgttcgggc gaagcgtatt
    16441 cgggcgtcat catccgcatc gcggtcgccg tcggctcctg agtttcgtga ataaggtcgg
    16501 gatttaaaag cttggcgatg ccgaaatcga gcagcttcgg aacgccgccc tcggtgacga
    16561 gaatgttcga cggcttgatg tcgcggtgaa taatcaggtt ttgatgcgcg taataaaccg
    16621 ccgcgcaaat ctgttggaaa agctgaag
//
LOCUS       DASWNE010000966         2955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_243655, whole genome shotgun sequence.
ACCESSION   DASWNE010000966 DASWNE010000000
VERSION     DASWNE010000966.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243655"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..821
                     /gene="rpsD"
                     /locus_tag="VGB68_18815"
     CDS             183..821
                     /gene="rpsD"
                     /locus_tag="VGB68_18815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEY0051356.1"
                     /translation="MARYREAVCRLCRREGAKLFLKGDRCFKPSCPIEKRGTQPPGMH
                     GATARRKVLVGYGQQLREKQKVKRIYFVLEKQFRNYFEKASRQKGITGENLLFLLERR
                     LDNVVFRAGFSTSRRQARQLVNHGHVEVNGRKVDIPSFQVKQGDIIVIKEKSVNNPHV
                     EGAWSTAGGRGRPQWISAGASDALSVTVSGLPTRADIDATINEQLIVELYSK"
     gene            925..1944
                     /locus_tag="VGB68_18820"
     CDS             925..1944
                     /locus_tag="VGB68_18820"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003513.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEY0051357.1"
                     /translation="MTTNNLWTDFQMPNRLEVEKETLTERYGKFYAQPFERGFGTTIG
                     NSLRRALLSSIEGAAITAVKIEGVEHEFSSIKGVVEDATDVILNLKQIPFKLHGAGSK
                     TLTISKSEAGEVTSEDIDHDGDVEILDQTVHIATIGAGGELNIEMRLKHGRGYVSAEF
                     NNDEDLSLGYIPIDSVHTPIKKVNYTVEATRQGSNTEFDKLTIEVWTDGSVKPDDSIG
                     LAAKLVKDHMTIFINFEEEEDEYKYEDIARPPLMRNDLLDRSVDELELSVRSYNCLKN
                     ADIRSIRDLVHRTERDMMSTKNFGKKSLNEIKDLLHGMGLDFGMDFDEQGNPIPGSGG
                     RDLAD"
     gene            2031..2534
                     /gene="rplQ"
                     /locus_tag="VGB68_18825"
     CDS             2031..2534
                     /gene="rplQ"
                     /locus_tag="VGB68_18825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEY0051358.1"
                     /translation="MRHLKAHRKLGRTTEHRISLLRNLATSLINAEKEQIVTTVPKAK
                     ELRSFVEKAITLARKAQNLDGDDAKARAVYLRRQAARFFHAGNRTHKTELSRFRGKKG
                     EPKERPERTAGVAAVQRLFGELGTRYKDRNGGYTRIIKLGRRQGDNAEMAVIQLVDNP
                     QELAAKK"
     gene            2623..>2955
                     /locus_tag="VGB68_18830"
     CDS             2623..>2955
                     /locus_tag="VGB68_18830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051359.1"
                     /translation="MSQQATSAKKETTKKATKSSRAAKATEPSLNMDELRELAELVDA
                     HGFTDFEFENETIRVRLSKQMAPPQIVQAVQPVAPVQSAASPAAPAATKLETPHPGAK
                     ADAEAAADE"
BASE COUNT          813 a          728 c          789 g          625 t
ORIGIN      
        1 cacaacggat gtcgtccgcc gaaaagaaga agagtttgag aattttcgat cggcgatttt
       61 gcgttggaat cgaaaatttg atttaagttt taattatcta attttatcga aaggatgaag
      121 ggcggatttt attaatccgt ctgtaaactt ttccgtcaaa attacggagg cagaaatatt
      181 taatggctag atatagagaa gcggtgtgcc gtctgtgccg ccgcgaaggt gcaaaattat
      241 ttttaaaggg cgaccgttgc ttcaaaccgt cgtgcccgat tgagaaacgc ggaacgcagc
      301 cgccgggaat gcacggcgcg accgcacggc gcaaagtttt agtcggctac ggtcagcagc
      361 ttcgcgaaaa gcagaaagta aaacgcatat actttgtgct ggaaaaacaa ttccgcaatt
      421 atttcgagaa agcctcgcgc cagaaaggca tcacgggcga aaacctgctc tttttgttgg
      481 aacgccgtct ggataacgtc gttttccgag ccggtttttc cacgtcccgc cgtcaggctc
      541 gccaattggt caatcacggt cacgtcgaag tcaacggacg caaagtcgat attccctctt
      601 ttcaggtgaa acagggcgac atcatagtga ttaaagaaaa aagcgtgaat aatccgcacg
      661 tcgaaggcgc gtggtcaacc gccggcggtc gcggtcgtcc ccagtggatt tcagccggag
      721 cgtcagacgc cttgagcgtg actgtttcgg gcttgccgac gcgcgccgat attgacgcga
      781 ccatcaacga gcagttaatc gtcgagcttt acagtaaata acagttaaca gagttgactg
      841 agttcagcga gttgaccgag ttattttaac tcggtcaact ttcagtcaac tcggtcaact
      901 cgctgaactt tttaatataa aaatatgaca acaaacaacc tttggacaga ttttcaaatg
      961 ccgaatcgct tagaggttga aaaagaaacc ttaaccgagc gatacggaaa gttttatgct
     1021 cagccgttcg agcgtggttt cggaacaacc atcggaaact cactgcgccg cgcgttgctt
     1081 tcgtcgattg aaggcgcggc aattacagca gttaagattg aaggcgtcga gcatgaattt
     1141 tcgtctataa aaggtgtggt cgaagatgcc accgacgtta ttttaaatct caagcagatt
     1201 ccgttcaaac ttcacggagc gggttcgaag accttaacca tcagcaaatc cgaagcgggc
     1261 gaagtgacca gcgaggacat cgaccacgac ggcgacgtgg aaattctcga ccagaccgtg
     1321 cacatcgcga ccatcggcgc gggcggcgaa ttaaatatcg agatgcgtct gaaacacggg
     1381 cgcggctacg tttccgccga gttcaacaac gacgaagacc tcagcttagg ctacattccg
     1441 attgactcgg tgcacacgcc gattaaaaaa gtcaattaca cggtcgaggc gactcgccag
     1501 ggctcgaaca cggaattcga caaactgact atcgaagtct ggacggacgg ctcggtcaag
     1561 cctgacgatt ccatcggtct ggcggcaaag ctcgtcaaag accatatgac catcttcatt
     1621 aattttgaag aggaagaaga cgaatataaa tacgaagaca ttgcgcgtcc gccgctgatg
     1681 cgcaacgacc ttctcgaccg ctcggttgac gagctcgagc tctcggtgcg ttcctacaat
     1741 tgtttgaaaa acgccgacat tcgctcgatt cgcgatttgg tgcaccgcac cgagcgcgat
     1801 atgatgtcaa ccaaaaactt cggtaaaaaa tcgctgaatg aaatcaaaga ccttctgcac
     1861 ggcatgggct tggatttcgg gatggatttt gacgagcagg gcaatccgat tcccggttcg
     1921 ggcggacgcg atttagcgga ctaaattaga aatgatgagc gcggaatggt gaacgctgag
     1981 taaaaatcaa aattcatcat tcatcgttca tcgttcatca ttttattgtt atgagacatt
     2041 taaaagcaca tcgtaaattg ggtagaacta cggagcatcg tatatcgctg ctgcgtaatc
     2101 tggcgacttc gctgattaac gccgaaaaag aacaaatcgt caccaccgtg ccgaaggcga
     2161 aagagcttcg ctcgtttgtg gaaaaagcca tcacgctggc gcgcaaagcg cagaatctgg
     2221 acggcgacga cgccaaagcg cgcgcagttt acctgcgccg tcaggcagcg cgatttttcc
     2281 acgccggaaa ccgcacgcac aaaacggaat tgagccgttt tcgcggtaaa aaaggcgagc
     2341 cgaaagaacg tccggaaaga actgccggag tcgccgcggt ccagcgtctg ttcggcgagc
     2401 tcggcacgcg ctacaaagac cgtaacggcg gctacacgcg cattatcaag ctcggtcgcc
     2461 gtcagggcga caacgcggaa atggcggtca tccaactggt tgacaatccg caggagttgg
     2521 cggcgaaaaa atagtaagcg gagtaggcag gagcagtaag caggcagtcg atttgtgtca
     2581 agtgcattgc ctactgcctt ctttctttta gaaggagcaa aaatgagcca gcaagcgaca
     2641 tcggcgaaaa aagaaacgac gaaaaaggcg acaaaaagtt cacgcgcggc gaaagcaacc
     2701 gagccttcgc tgaatatgga cgagctgcgc gagcttgccg aactggttga tgcacacggt
     2761 tttacggatt tcgaatttga aaacgaaacc attcgcgtgc gtctgagcaa acaaatggct
     2821 ccgccgcaga ttgtccaagc cgttcagcct gtcgcgcccg ttcaatcggc agcgtcgccc
     2881 gcagcgccgg cggcgacaaa gctggaaact ccgcatccgg gcgcgaaagc cgacgcggaa
     2941 gcggcggcgg acgaa
//
LOCUS       DASWNE010000967         2930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_563929, whole genome shotgun sequence.
ACCESSION   DASWNE010000967 DASWNE010000000
VERSION     DASWNE010000967.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563929"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..2604)
                     /gene="topA"
                     /locus_tag="VGB68_18835"
     CDS             complement(52..2604)
                     /gene="topA"
                     /locus_tag="VGB68_18835"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEY0051360.1"
                     /translation="MGKNLVIVESPAKAKTINKYLGSDYLVMASIGHIKDLPMKELGV
                     DIENNFEPTYEIIPKKGNRESKQKIVSALKKAAKDADVIYLAADPDREGEAICQHLAE
                     EVVPKKPAKPYYRVMFNEITKNAIKDAFKDPKQVNQNLVDAQQARRILDRLVGYKVSP
                     ILWKTIGGKLSAGRVQTVAVRMIVEREREIENFVQTEYWTIIANLSAQLPPKFDARLY
                     KIEDLTVKTGGFDQDLKKNETHIKDEQTAKSIVEEAEKENFAVDSVTTKERKRNPVPP
                     FITSKLQQEASRKLSFPVKKTMTVAQKLYEGVEVGAEGAVGLITYMRTDSTRVSDAAL
                     NDLRDFIGASYGAKYLPEKPVFYKTKGGAQDAHEAIRPTDANRTPDSLKNYLNADELK
                     LYTLIWKRFVASQMMPAIFDQTTIDIKAGRFTFRATGSVQKFDGFLKVYQEGRDEKPA
                     EGEDEDAELTLPKVEKGEPLKLNQITPEQHFTEPPPRYTEATLVKALEEKGIGRPSTY
                     AAIMTTIQDREYVERIESRFHPTPLGTTVNDMLVASFDDLFNETYTARMENQLDEIEE
                     GKLDWRNALRGFYDKFSKDLETAVETIKNKKQTSIPTNEICEKCGSGMVIKFGRFGQF
                     LACANYPECKNTREVGSKKTDGGSDAQAQGNGADAENKSDAQSAEVPNCELCGREMSL
                     KKGRFGSFYGCTGYPECKNIRKIAKGEQKPVAPPVELDEICPKDGVKLVRRQGRFGEF
                     ISCSNYPKCDYIKRETLDIPCTKCKTGEIAVKKSKRGKVFYGCTNYPKCDVVFWDKPV
                     TEPCPQCNAPFVLEKTTKKDGTIRYCDKCDYKTAAGDVPVSTESKDAVSLAG"
BASE COUNT          600 a          771 c          696 g          863 t
ORIGIN      
        1 agcacagaat aaaataatcc gtgaaaagcc gcgtcaaccc gtcgtttcca attagcccgc
       61 taaactaacg gcgtctttag attcggtcga gacgggcacg tcgcccgccg ccgttttgta
      121 atcgcatttg tcgcaatagc gaatcgtgcc gtctttcttc gtcgtttttt ccagcacaaa
      181 cggcgcgttg cattgcgggc acggctctgt gacgggctta tcccagaaaa cgacgtcgca
      241 tttcggataa ttcgtgcagc cgtaaaagac ttttccgcgc ttggattttt taacggcgat
      301 ttcgcccgtt ttgcatttcg tacagggaat atccaaagtt tcacgcttga tgtaatcgca
      361 tttcggataa ttcgagcagg aaataaattc gccgaagcga ccttgccgcc tgactaattt
      421 cacgccgtct ttcgggcaaa tctcgtccag ttcgaccggc ggcgcgacgg gtttctgctc
      481 gcctttggca atcttgcgaa tgtttttaca ttcgggataa ccggtgcagc cgtaaaacga
      541 gccgaaacga ccttttttca aagacatctc gcgcccgcac agctcgcaat tcggaacttc
      601 cgccgattga gcgtcggatt tattctccgc gtccgctccg tttccctgcg cctgcgcgtc
      661 gcttccgccg tccgtcttct tgcttccgac ttcccgcgta tttttacatt cgggataatt
      721 ggcgcaagcc agaaactgcc cgaagcgtcc gaacttgata accattcccg aaccgcattt
      781 ttcgcagatt tcattggtcg gaatcgaggt ttgttttttg ttctttatcg tctcgacggc
      841 ggtttccaaa tccttcgaga atttgtcgta aaagccgcgc agagcgttgc gccaatcgag
      901 cttgccttct tcgatttcgt caagctgatt ttccatccgc gccgtgtaag tctcgttgaa
      961 caaatcgtcg aagctcgcca cgagcatatc gttgacggtc gtgcccaaag gcgtcgggtg
     1021 aaagcggctt tcgattcttt cgacgtattc gcggtcttga atcgtcgtca taatcgccgc
     1081 gtaagtcgaa ggtctgccga tgcctttttc ttccagcgcc tttaccagag tcgcttccgt
     1141 atagcgcggc ggcggctcgg taaaatgctg ttccggcgtg atttgattca gtttcaacgg
     1201 ctcgcctttt tcgactttcg gcagagtcag ctcggcgtct tcgtcctcgc cttccgccgg
     1261 tttttcgtcg cgcccttcct ggtaaacttt cagaaagccg tcgaatttct gcaccgagcc
     1321 ggtcgcgcga aaagtaaagc gtccggcttt aatgtcaatc gtcgtctggt cgaaaatcgc
     1381 cggcatcatc tgcgaggcga caaagcgttt ccagataagc gtgtaaagct tcagctcgtc
     1441 ggcgtttaag taatttttca ggctgtccgg cgtgcggttt gcgtcggtcg gtcgaatcgc
     1501 ttcgtgcgcg tcctgcgcgc cgcccttggt tttatagaaa accggttttt cgggcagata
     1561 ttttgccccg taactcgcgc cgataaaatc ccgcaggtca tttaaagccg cgtcggaaac
     1621 gcgcgtcgaa tcggtgcgca tatacgtaat cagaccgacc gcgccttccg cgccgacttc
     1681 gacgccttca taaagcttct gcgcgacggt catcgtcttt ttgaccggaa aacttaattt
     1741 gcgcgaggct tcctgctgca atttcgaggt gataaacgga ggaaccggat tgcgcttgcg
     1801 ctctttcgtc gtgaccgaat cgaccgcgaa attttccttt tccgcttctt cgacgattga
     1861 ttttgccgtc tgctcatctt tgatgtgagt ttcgtttttc tttaaatcct ggtcaaagcc
     1921 gccggttttt acagttaaat cttcaatctt ataaagccgc gcgtcgaatt tcggcggaag
     1981 ctgcgcgctc aaattggcga taatcgtcca atattcggtc tgcacgaaat tttcgatttc
     2041 gcgctcgcgc tcgacaatca ttctaacggc gaccgtttgc acgcgtcctg ccgaaagctt
     2101 tccgccgatg gttttccata aaataggcga gactttgtag ccgaccagcc tatcgagaat
     2161 gcggcgcgcc tgttgggcgt cgaccagatt ttgattgacc tgcttcgggt ctttaaaagc
     2221 gtctttaatc gcgtttttcg tgatttcgtt gaacatcacg cgataatatg gtttagctgg
     2281 ttttttcggg acaacttctt cagcaagatg ctggcaaatt gcttcgcctt cacggtcagg
     2341 gtcagccgcg agataaatta cgtcagcgtc tttcgctgct ttttttaacg cagaaacaat
     2401 tttctgctta ctttctctgt ttcctttttt tggaataatc tcataggttg gctcaaaatt
     2461 attctcgatg tccacgccga gttctttcat cggcaaatcc ttgatatgcc cgatggaagc
     2521 cattaccagg taatcgcttc cgagatattt gttaatcgtt ttcgccttcg cgggcgattc
     2581 gacaatgact agatttttgc ccattttttt gaaaaaaata tgtcagtgcg tttcccacgc
     2641 ctgacatttg acttctaaca cttgcctgag ccccgtgtca atctaaggtt aaaatttctt
     2701 tactttgctt tatcgtaaat ttcctgttta tgtcatttct tcggatgaag atttaccggc
     2761 tttagttcgc cgcaatcggg ctgacgccca acaagcggaa gcccgcgcaa aggattcggg
     2821 attcaggatt caggattcag gatttaagat tcaggattca ggattcagga tttaagattc
     2881 gggattcagg attggtcgcc tgctaatggt aacttccaaa tcctgaatcc
//
LOCUS       DASWNE010000968         1803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_383565, whole genome shotgun sequence.
ACCESSION   DASWNE010000968 DASWNE010000000
VERSION     DASWNE010000968.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383565"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(339..>1803)
                     /locus_tag="VGB68_18840"
     CDS             complement(339..>1803)
                     /locus_tag="VGB68_18840"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEY0051361.1"
                     /translation="NKIRLTNNGFNDHLPRWSPDGSKIAVGRCNADYLSCDIVVMNAD
                     GTEQNNLTSGNMGDDDTPQWTPDGSKIVYVAQNSSLDYNAFIVDVDGTNQMPLTNAAA
                     PQLYQIGQVSLNGKVGLQSSAVAIDTFEIATVGIDGNNLQNLTLNNVYDAFGRWSPDG
                     SRIAFISRRDTNNGEIYLMNADGNNIVRLTNNTAGESITDWRAAPAPTRAPFDFDGDG
                     KADLAVFRPTGGNWYISNSSNGSLIAVQFGAAGDLIAPADFDDDGKTDISVFRPAGGN
                     WYRLNSSNNTFSAAQFGANGDLPVPGDFDGDRKADICVYRPSAGSWYRVNSSNNQFVA
                     AQFGANGDKPLVADFDGDGKSDLAVYRPPEGTWYYINSSNDSFSAAQFGLVNDKPVPA
                     DYDGDRKDDLAVFRPSPHGPAWYRINSGNGAFVPTDFGVFGDLPVPSDYDGDGKADLA
                     MFNPVNGGWYMRRSTAGPFSIQFGAQGDKPAPGAFVR"
BASE COUNT          421 a          462 c          475 g          445 t
ORIGIN      
        1 cggaacttca ttttcagcct aaatcattaa acggctttta taaaatttaa gacgcgccgc
       61 ttgtaatttt gcaggcggcg tatttttttt cataaatttg cggaagtctt tttcgattga
      121 aactatagct tagtattcaa aacaatcgga cggactgaag gcggagaagg caacgggcgg
      181 ggtctttctt taaggaaaag agaaagaccc gaacgggcag ggagaatctc ccgatttccg
      241 gcaattttcg tccggtttaa tttgaatatt ttgacgccga aaacaaaaaa gacctgagaa
      301 gtaatttcag gtcttttttg taatttaccg acgctttttt atctgacgaa agcgcccgga
      361 gccggcttat cgccttgcgc gccgaattgt atgctgaaag gtcctgccgt cgagcgccgc
      421 atataccaac cgccattcac aggattgaac atggctaaat cggcttttcc gtcgccgtca
      481 taatcagatg gaacgggcaa atctccgaaa acgccaaaat cggtcgggac aaacgcgccg
      541 tttccgctgt tgatacgata ccaggcgggt ccatgaggag aaggacggaa aaccgccaaa
      601 tcgtccttgc ggtcgccgtc atagtctgcc ggaacaggct tgtcattgac caagccgaat
      661 tgagcagccg aaaatgaatc attgctgctg ttaatgtaat accaagttcc ttcgggcgga
      721 cgataaacgg ctaaatctga tttgccgtcg ccatcgaaat ccgcgacgag cggtttatcg
      781 ccgtttgcgc cgaactgcgc ggcgacgaat tgattgtttg agctattcac gcgataccag
      841 cttccggctg acggtcgata aacgcaaata tcggctttcc ggtcgccgtc gaaatcgccc
      901 ggcacgggca aatccccgtt tgcgccgaac tgcgccgccg agaaagtgtt gttcgacgag
      961 ttcaggcgat accagtttcc gccggcggga cgaaagacgg aaatatcggt ttttccgtcg
     1021 tcgtcgaaat cggcgggcgc gattaaatct ccggcggcgc cgaactgcac cgcaatcagc
     1081 gagccgttcg agctgttgct gatataccaa tttccgccgg tcggacgaaa aacggcgagg
     1141 tcggctttgc cgtcgccgtc aaagtcgaac ggagcgcgcg tcggcgcagg cgcagcccgc
     1201 caatcggtga tactctcgcc ggcagtgtta ttagtcaacc ggacgatatt gttgccgtcg
     1261 gcgttcatca ggtaaatttc gccgttgtta gtgtcgcggc gactgatgaa agcaatcctg
     1321 gagccgtcag gcgaccatct tccgaaagcg tcgtaaacgt tgttaagcgt cagattctgc
     1381 aaattgttcc cgtcgattcc gaccgtcgct atttcaaaag tatcaattgc gactgcgctg
     1441 gactgtaagc cgacctttcc gtttaatgaa acctgaccta tctggtaaag ctgcggagcc
     1501 gcggcattcg tcagcggcat ctggtttgtt ccgtccacat ccacgataaa ggcgttataa
     1561 tccagagacg agttttgtgc cacatacacg attttactgc cgtccggcgt ccattgcgga
     1621 gtatcgtcgt cgcccatatt gccggaggtg agattgtttt gctccgtgcc gtccgcattc
     1681 atcacgacga tatcgcagct tagataatcg gcgttgcagc gtccgaccgc tattttgctg
     1741 ccgtccggcg accatctggg aagatggtca ttaaagccgt tatttgtcag gcggatttta
     1801 ttg
//
LOCUS       DASWNE010000969         5051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_525642, whole genome shotgun sequence.
ACCESSION   DASWNE010000969 DASWNE010000000
VERSION     DASWNE010000969.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5051
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525642"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /locus_tag="VGB68_18845"
     CDS             complement(<1..1302)
                     /locus_tag="VGB68_18845"
                     /inference="COORDINATES: protein motif:HMM:NF025670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HEY0051362.1"
                     /translation="MQTRTAFSRTALAATFNLKTKGKKAFYAILTLIFALSLSVIWLA
                     NGVSSQAEELKNLRGEDAVKRLRQNGQYDSLTKAFSAVRRAERVQNDSASFTASEAMP
                     PTAKLYAPDGAPANFFGYSVALSGDTAIVGVPLDSLESINPASVSEGSAYIFTRSGTS
                     WTLQQKLTAPDGSNRDYFGANVSIDGDTAVVGALFDDVGANANQGSAYIFTRSGTVWT
                     QQAKLTAAQGAAGDRFGASVEISGETIVVGTKRDQGAAYVFTRSGTTWTQQQQLTPSN
                     GAAGDEFGASVAINVDTILVSAAGRPVSGADNQGAVYVFTRAGGVWSEQQILTAVDGA
                     AGDRFGSDLAITGDTAIIGANFDDIGGAADQGSAYIFTKSGSVWTQQAKLLAADGAAG
                     DHFGSSVALGGNTAVVGAPDNDFPGILDDIYVNAGSAYVFTG"
     gene            complement(1645..1717)
                     /locus_tag="VGB68_18850"
     tRNA            complement(1645..1717)
                     /locus_tag="VGB68_18850"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1685..1687),aa:Arg,seq:acg)
     gene            complement(2142..3266)
                     /locus_tag="VGB68_18855"
     CDS             complement(2142..3266)
                     /locus_tag="VGB68_18855"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY0051363.1"
                     /translation="MDAAKVIRKSFKFRIYPTKAQTVKLAQTLELCRELYNAGLQERR
                     DAWKLNKVSINYFDQQNQLPEIKSIRDDLSSIHSQILQDVLKRVDKTFKAFFNRIERK
                     EKAGFPRFKGRNRFDSFCFPQSGFTLTGNKLSLSKIGKVKIKFSREITGKVKALTVKS
                     ECGKWFVIFSVETATAPLPKTNKEIGLDAGIESFITLSDGTKIENFKYFESTEKKLRV
                     AQRKAARRKIGSNRRRKAVLLLKKIYAKIKNQRNDFQHKVSTRLVKDFDLIAIEKLNI
                     LGMSRSILAKQVNDAAWHSFFQKLKYKAENAGKSVIEVNPNFTSQDCSACGDRVKKRL
                     SERSHHCPKCGLKIHRDENAALNILKLGQSFLAQTKAVRL"
     gene            complement(4022..4192)
                     /locus_tag="VGB68_18860"
     CDS             complement(4022..4192)
                     /locus_tag="VGB68_18860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051364.1"
                     /translation="MKALQRRLSRTFLKMTKHYEKLTPEKKVKLWYLLEIVERELDAI
                     LADDTTEKTQKD"
     gene            complement(4460..4660)
                     /locus_tag="VGB68_18865"
     CDS             complement(4460..4660)
                     /locus_tag="VGB68_18865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051365.1"
                     /translation="MKANKTTIRKGDTVTATFAWTENEQPSDKIFFTGKVKNVIRKRG
                     GIIYFELENLSREVPADRVQLA"
     gene            4812..5024
                     /locus_tag="VGB68_18870"
     CDS             4812..5024
                     /locus_tag="VGB68_18870"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY0051366.1"
                     /translation="MISSVIKVKLKELLKEKDKTLLSLARETGISYNTLHKIKKNDVQ
                     SMSFDVLEKICKNLKCSTSDLLAIEE"
BASE COUNT         1260 a         1228 c         1075 g         1488 t
ORIGIN      
        1 gcccgtaaaa acatacgcgg aacccgcatt gacatatata tcgtctaaga tgccgggaaa
       61 atcgttgtcg ggcgcgccga cgaccgccgt attgccgccg agcgccacgc tcgaaccgaa
      121 atggtcgccc gccgcgccgt cggcagcgag cagtttcgcc tgttgcgtcc acacgctgcc
      181 gcttttcgta aaaatataag ccgagccctg gtcggctgct ccgccgatgt cgtcaaaatt
      241 cgcgccgatg attgccgtgt cgcccgtaat cgccaagtcg ctgccgaaac ggtcgcccgc
      301 cgcgccgtcg acggcggtta aaatttgctg ctcgctccag acgccgccgg cgcgcgtaaa
      361 aacatacacc gcgccctgat tatctgcgcc cgacacgggg cgacccgccg ccgaaacgag
      421 aatcgtatcg acattaatcg ctacgctcgc gccgaactcg tcgcccgccg cgccgttcga
      481 aggcgtcaac tgctgctgtt gcgtccacgt cgttcccgaa cgtgtaaaaa catacgccgc
      541 gccctggtcg cgctttgttc cgacgacgat tgtttcgccg ctgatttcca cgctcgcgcc
      601 gaagcggtcg cccgccgcgc cctgcgccgc cgtcagcttc gcctgctgcg tccagacagt
      661 tcccgaacga gtgaaaatat atgccgaacc ttgattggcg ttcgcgccga catcgtcgaa
      721 cagagcgccg acgactgccg tgtcgccgtc aatcgagacg ttcgcgccga aataatcccg
      781 gttcgagccg tccggtgcgg tcagcttttg ctgtaatgtc cagctcgtgc cgcttctcgt
      841 gaaaatgtaa gccgagcctt cgctgacgct cgccggattg attgattcga gactgtcgag
      901 cggcacgccg acgattgctg tgtcgccgct gagagcgacg ctgtaaccga aaaagtttgc
      961 gggcgcgccg tccggggcat acagcttcgc cgtcgggggc atagcttcgg acgcggtgaa
     1021 agaagccgag tcattctgaa cgcgctcggc gcgccgcacc gcgctgaacg ctttcgtcag
     1081 cgaatcatac tgcccgtttt gcctgagccg tttgacggcg tcttcgccgc gcaggttctt
     1141 caattcttcc gcctgcgacg aaacgccgtt cgccagccaa atcacgctca gcgataacgc
     1201 gaaaatcaaa gtcaatatcg cataaaatgc cttttttcct ttggtcttca aattgaatgt
     1261 cgctgctaaa gcagttctgg aaaaagccgt tctcgtttgc ataaatctct ccggtttaaa
     1321 ttttattttt gcggtcttgc aagtatatgc gtaaaaaccc ggctaagtca gccaggattt
     1381 ccgaaaaatt ttatttcggg gaaataaacc taaaatacag cgtcaatatt tcaaacagag
     1441 attataaata acggcaagaa ggcggtgtat ggaaagacgg cagcgcggat ttcggcaacc
     1501 gcctcgccag gttcccggcg ctatataaat cacgctgtca cggaaatttc ctttcgcgaa
     1561 actataaatt acaagaaatt aacttttatg gtgtgagtca atggaaaaag gcatcaaccg
     1621 atgcctttaa ctggttaaaa ataatggtac acccggcatg attcgaacat gcgacctctt
     1681 gattcgtagt cagattcgtt gttgcttttc cgcctgcccg caaatcgctg acaaattaaa
     1741 cctttagatt gtttcccagc ataccccgaa aaaaccgaat ctacatattt atctacatag
     1801 tttttaaaaa ttttcggttt ttttaagttt tgaacgtcaa aatatacttt aagtatagtt
     1861 gccgaccgga ctattaccga cgattcggac tgtctccggc aaagttaccg gtcggcgtgt
     1921 ttccgatggc gggcgcgctc atatttccgc aaaagtttcc ggtcggcaaa tctccgcgca
     1981 tatttatttc tcctttattc gtcttcgtcg aaaaattccg ccagctcccg gtaatcatcg
     2041 tccgtttcaa tccgcgacga aaacaaatcg tcgagcgcga ttaaatcgaa ttccgagatt
     2101 gacattccgc accttttaac gagcgcggat tctctgacaa ctcataacct aacggcttta
     2161 gtttgcgcca gaaagctctg tccgagcttc aaaatgttca aagcggcgtt ttcgtctcga
     2221 tggattttta agccgcattt cgggcaatga tgcgagcgtt cagacaagcg tttttttacc
     2281 ctgtcgccgc aagcagaaca gtcctgactc gtaaaatttg gatttacttc tattactgac
     2341 ttaccagcat tttcagcttt atatttcagt ttttgaaaga agctatgcca agccgcatca
     2401 ttgacttgtt tggctaaaat acttctcgac attccgagaa tgttaagttt ttcaatcgct
     2461 atcaaatcaa aatctttgac cagacgagtt gaaactttat gctgaaaatc gtttctttgg
     2521 tttttaattt tcgcataaat ctttttcagc agcaaaaccg ctttgcggcg gcgattactg
     2581 cctatttttc gccgcgctgc tttgcgctgt gcaactctta atttcttttc agtcgattca
     2641 aaatacttga agttttcaat cttcgttccg tccgaaagcg taataaatga ttcgattccg
     2701 gcgtccaatc cgatttcttt atttgtcttc ggcagtggcg cggtggcggt ttctaccgaa
     2761 aaaatcacga accattttcc acactcgctt ttgactgtga gtgctttaac tttgccggta
     2821 atttcacgcg aaaacttgat tttaactttt ccgatttttg ataaagaaag tttattgcca
     2881 gttaaagtga atccgctttg cggaaaacaa aaagaatcga atctattcct gcctttaaat
     2941 cttggaaatc cggctttttc tttcctttcg attcgattaa agaatgcctt gaaagtttta
     3001 tccactcgtt tcaaaacgtc ctgcaaaatc tgggaatgaa tcgaacttaa atcatcacgg
     3061 attgatttaa tctccggtag ttggttttgc tggtcgaaat aattaatcga aactttattc
     3121 agtttccagg cgtcgcgcct ttcctgtaat ccggcattgt ataattcgcg gcaaagctcc
     3181 aatgtctgcg ccagcttcac agtttgcgct ttagtcggat aaatccgaaa cttgaatgac
     3241 tttctgataa cttttgccgc gtccattttt atttacttac ctatataata taaacggtta
     3301 aaacctgtgt ttttccattg ttttcaataa cttaaaactc tttttctgtg tttattttct
     3361 tccgtaaaaa tcaagttctt gacgtataat cgacgcgcta actcgtaatt gccgtttgcc
     3421 ctgaatttcg cctcacaaag ccgacaacga cgcggcgcgg tctggaaatt gtgtcgaaaa
     3481 cattttaatc tatccattta aaactttatt tccttttgac gaattgcaat ttttacaagc
     3541 gatttttgcg ttattcggac aatgaaaacc accctgtgaa agcggtataa cgtggtcaat
     3601 cgttgcggtc tgataagtca gtaattttcc gcataagtag caattcaatc cgtcgcgcct
     3661 gattatttcc gattttgtaa aaggaattat cacgctcgcg cccgctaatc ttgcccgcct
     3721 tgtcattaga ttcttttctc tgatttccgg ctttagcggt ttccagaatc tttgaaacgg
     3781 cttcgccagt tttctacacg gtcggcatct ttcggcgtaa gtccaaaaac agccgcgctt
     3841 gaccacgaac atatttaaag gcttttcaag ctgacagttt ttgcagaatt ttgttttcat
     3901 aaattaggtg aggcgcgtct ttcgtaattt ttagtcggaa agctgatagc gcccctcgcc
     3961 tgaaaagata aagcacctac gaaatgcctc tcttttcgtc cgccttttgc cagtgcggaa
     4021 actaatcttt ttgtgtcttt tcggttgtat cgtccgccag aatcgcgtcc agctcgcgct
     4081 cgacgatttc cagcaggtac cacagcttca ctttcttttc gggcgtcaat ttctcgtaat
     4141 gtttcgtcat tttcagaaac gtgcggctca agcgcctttg tagtgctttc atctcgcgcc
     4201 gcctcgcttt ttaatctcga aacgatgtgc ggcgcgtttt aataaatccg cgtccgttac
     4261 gggaaaaaca gctttcacac aaagcgtccg atttgcgctc gtttcgctaa acaaaagggt
     4321 caaaatgtaa ttaactaaat ttaaaaatga atggaattta agtttgattc gtagtatatt
     4381 tttcacgtgt atatctcctg attttaatta ggggaaagag aggcttaggc aagttggcgc
     4441 ttacctaagc ctttcgcttt tatgccagtt gaacacggtc ggctggaact tcacggctca
     4501 agttttcgag ttcaaagtaa ataatcccgc cgcgtttgcg aatcacgttt ttgactttgc
     4561 cggtgaaaaa aattttgtct gacggctgct cgttttcagt ccacgcgaaa gttgcggtga
     4621 ctgtatcgcc ttttctgatt gtggttttgt tggctttcat tttttattct ccgttttgtt
     4681 tatttaatgt aatatcctgc cgacaagata aattatactg ctaacgatat aaaaatacaa
     4741 gcaaaaaaac tactgtcaat aggataaatt atcctattga caagatattt aaaaaatgtg
     4801 ataattttgc tatgatttcg agcgtgataa aagtaaaact taaagagctt ttgaaggaaa
     4861 aagataagac tttactttct ttagcaagag agacgggcat ctcttataat actttgcaca
     4921 aaattaagaa aaacgatgtg caaagtatgt cttttgacgt tctggaaaag atttgtaaaa
     4981 accttaaatg ctcgaccagc gatttattag cgattgaaga ataattctat ccgacaaagt
     5041 aaaatttgac g
//
LOCUS       DASWNE010000970         1743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_383601, whole genome shotgun sequence.
ACCESSION   DASWNE010000970 DASWNE010000000
VERSION     DASWNE010000970.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383601"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..977)
                     /gene="tpiA"
                     /locus_tag="VGB68_18875"
     CDS             complement(213..977)
                     /gene="tpiA"
                     /locus_tag="VGB68_18875"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEY0051367.1"
                     /translation="MRKPVIAGNWKMYKMLDEAVQTALALKSLVANANHCEVVIAPVY
                     VHLKTLADRLEGSNIRISAQNCAAEQKQSANTGEVSAEMLKDAGCTHIIIGHSERRQF
                     YGETDKSVSEKTRAALAAGLTAIVCIGETLQERESEKLFEVVERQLRAGLAGLTTADM
                     ERIIIAYEPVWAIGTGKTATPEQAQEMHGFIRRKLTETHSEETAQATRILYGGSVKPE
                     NIKILMSQADVDGALVGGASLEAESFAQIVNYKGSE"
     gene            complement(1144..>1743)
                     /locus_tag="VGB68_18880"
     CDS             complement(1144..>1743)
                     /locus_tag="VGB68_18880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051368.1"
                     /translation="NVRWLYRFIDASGAIASDLLAINYGKYEKLYGKNATGKRFLDVL
                     ENLGKDDSIRAVDVLVMIHGLDKKLIFADAGFDTAELSAEIRKLNLSNKLRLLYSTAC
                     YGLSHAGDFVNAGFSAAVGAKKVNANAAAEVPVLFTMWAGGSRLREAVAKGESPTTRL
                     PSDKAAGWFGKMNKTSWRDDVDSDKVIIGNDSITIKSDA"
BASE COUNT          407 a          481 c          393 g          462 t
ORIGIN      
        1 aatcgtctgc gttccgcgcg gaccgaaagc cgagctggaa atattgctgg taaacaaagc
       61 gcccgcgtcg gttttgcccg gctgcaaaag cacagtcgca atcaaaacga tacaggaaat
      121 aaaaaatagt gcgtaaagta aataagctaa catctgaaaa accgtgaatc atccgttcgc
      181 gaacgattaa taaaaacctt tcgcttccgg cgttattcac ttcctttgta attcacaatc
      241 tgcgcgaagc tttccgcttc caggcttgct ccgccgacta atgcgccgtc gacgtcagcc
      301 tgcgacatca aaattttgat attttctggt ttgaccgaac cgccgtaaag aattcgcgtg
      361 gcttgcgccg tttcctccga gtgcgtttcc gtcaatttcc ggcggataaa gccgtgcatt
      421 tcttgagcct gttcgggcgt ggctgtttta ccagttccaa ttgcccaaac aggctcgtaa
      481 gcgattatga tacgttccat atcggcggtt gtcaaaccag ccaaccccgc tcgaagctgt
      541 ctttcgacca cttcaaaaag tttttccgat tctctttcct gaagcgtttc gccgatgcag
      601 acaatcgccg tcaaacccgc cgccagagcc gcccgcgtct tttccgacac gcttttgtcc
      661 gtttcgccgt aaaactgtcg ccgctcggaa tgaccgataa ttatgtgcgt gcagccggcg
      721 tccttgagca tttcggcgga aacttcgccg gtgttcgcgc tctgcttttg ctcggcggcg
      781 caattctgcg ccgaaattcg tatattcgag ccttcgagcc ggtcggcaag cgtttttaaa
      841 tgaacgtaaa cgggcgcgat gacgacttcg caatgattcg cgttggcgac cagagacttt
      901 aaagcgagcg ccgtctgcac ggcttcgtca agcattttat acattttcca gtttcccgcg
      961 atgacaggtt ttctcattga ataaatttat ccgaaaaata atttggtaaa gcttaggtta
     1021 attttatcac aaacaaaaac ggcttggatt cgccaaaccg attttcgcaa actaaaattt
     1081 taaaacttta cggcggcgca caatattttc taaatagtta ttttcggcgc tttcacgggt
     1141 aaatcaagcg tctgatttga tggtgatgct gtcgtttccg atgattacct tgtcgctgtc
     1201 tacgtcgtcg cgccaactgg ttttgttcat tttcccgaac catccggctg ccttgtccga
     1261 cggcaggcgg gtggtcggcg attcgccttt cgcgaccgcc tctctgagtc gtgaaccgcc
     1321 agcccacatc gtaaacaaga ccggaacttc ggcggcggca ttggcattga ctttcttcgc
     1381 tccgaccgcc gcgctaaaac cggcgttgac gaaatctccg gcgtggctca agccgtaaca
     1441 ggcggtcgaa taaagcagcc ggagcttatt cgataagttc aatttccgta tctccgccga
     1501 tagctccgcc gtatcaaatc cggcatctgc aaaaatcagt tttttgtcca aaccgtgaat
     1561 catcaccagc acgtctactg cccgtatgga atcgtctttt cccaaatttt caagcacatc
     1621 gagaaaccgc tttccggtcg catttttccc gtagagcttc tcgtatttcc cgtaattaat
     1681 cgccagcaaa tccgaagcaa tcgctcccga agcgtcgata aaccggtaaa gccaccgcac
     1741 att
//
LOCUS       DASWNE010000971         1920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_31856, whole genome shotgun sequence.
ACCESSION   DASWNE010000971 DASWNE010000000
VERSION     DASWNE010000971.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31856"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            592..>1920
                     /locus_tag="VGB68_18885"
     CDS             592..>1920
                     /locus_tag="VGB68_18885"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEY0051369.1"
                     /translation="MFRPNRLFLFFTFVTLSFFTLTGCKSNTAEEIPSAPTQIQPQAP
                     PAPVVGDGSRLTYADVVDRVAPAVVMVTIEQRAKPSQQREAPFLDNPFRDLIPQQPQQ
                     PQRPQRGIGSGVIVSADGTILTNNHVIEGADKIRIELNDNRTFEARVVGTDPLSDLAV
                     VKIEAQNLPFLNLGDSDRVRVGDIILAIGNPLGIGQTVTAGIISAKGRRTGISDGSFE
                     DFLQIDAPINRGNSGGALVNLSGELIGINSQILAAPGGSGGNIGIGFSIPSNMAKSVM
                     EQLVKEGKVRRGKIGISIQNPTEEMREALGLKDTKGVLVGGVQAGSAAEKAGIRRGDV
                     IIAVNGEKVEDSNILRNRIAGTAPGTEINLTIMRDGSEQEVRATLDELKSEDAANSTN
                     PEENEKEPEKSAEGGKLGLDLQPLTPEMARRLKIPDTRGMLITGVDPNGAS"
BASE COUNT          503 a          500 c          508 g          409 t
ORIGIN      
        1 tctaaaaccg acaattccga cgcgcccgct tttctctgcg ccaagctcgt ctataaatcg
       61 ctggcatata tctttcaaaa cgacacgccg aaagttcgcg gaaaatctac ataaccgcga
      121 aatagctcga aaaaagcgga aacgagacgg ttccggacaa acaatcgact ttttaagtca
      181 ttcaatgcat ctcaaaaggc attcaaagcc ctgtccaggt cgttcaacag acaattcaag
      241 tcgttcaacg ggctgttgaa gtcattcaat gccttgttcg aatcgttcaa cgagcgattt
      301 caaccgttca ataaaccttt caagtcgtgc aacgaacttg aaatatcatt caaaaggctt
      361 ttcggggcgt tcaaagtgtt ttgcgagccg ttctattttt ccggacgccg aagtttataa
      421 aaagtttata aagatttgcg aatctggagc tttttttcaa attgtcatga acaaaaacga
      481 tttgtttctc gtaaccaatt cagtaaattc aaaagtattt cacgctttca atatttttcc
      541 gctttgaagt aaaattctta taagcttatt ttgaaagatt gagaggttaa aatgttcaga
      601 ccaaacagat tatttttatt ctttacgttc gtcacgcttt catttttcac gctgaccggc
      661 tgtaaatcca ataccgcgga agaaattccg agcgcgccga cgcagattca gccgcaagcg
      721 ccgcccgcgc cggtcgtcgg cgacggctcg cggctgactt atgcggacgt tgtcgaccgc
      781 gtcgctccgg cggtggtgat ggtcactatc gaacagagag ccaagccttc gcagcagcga
      841 gaagctccct ttctcgataa tccgttccgg gatttaattc cgcagcagcc ccagcagccg
      901 cagcgaccgc agcgcggaat cggttcgggc gttatcgtca gcgccgacgg aacgattctg
      961 accaacaacc acgttatcga aggcgcggac aaaatccgca tcgagctgaa cgacaaccgc
     1021 acttttgaag ccagggtcgt cggcacagac cctctgagcg atttggcggt cgttaaaatc
     1081 gaagcgcaga atctgccgtt tttaaatctc ggcgattctg acagggtgcg cgtcggcgac
     1141 atcattctgg caatcggaaa tccgctcggc atcgggcaga cggtgacggc gggaattatc
     1201 agcgcgaaag gtcggcggac gggcatcagc gacggcagct tcgaagactt tctgcaaatc
     1261 gacgcgccga ttaatcgcgg caattccggc ggcgcgctgg taaacctgag cggcgagcta
     1321 atcggcatca actcgcagat tctcgccgcg ccgggcggtt ccggcggcaa catcggcatc
     1381 ggtttttcga ttccgtcgaa tatggcgaag tccgtgatgg agcagctcgt caaagagggc
     1441 aaggtacggc gaggaaaaat cggcatcagc attcaaaatc cgaccgagga aatgcgagaa
     1501 gcgctcggtt tgaaagacac taaaggcgtt ctggtcggcg gcgtccaagc cggcagcgcg
     1561 gcggagaaag cgggcatcag gcgcggcgat gtcatcatcg ccgtcaacgg cgaaaaagtc
     1621 gaggacagca acatcctgcg caatcgcatc gccggaacgg cgcccggcac ggaaatcaat
     1681 ctgaccatta tgcgcgacgg cagcgagcag gaggtcagag cgactcttga cgagttaaaa
     1741 tcggaagacg cggcaaattc gacgaatccc gaagaaaacg aaaaagaacc ggaaaaatcg
     1801 gcggaaggcg gcaagctcgg tctcgattta cagccgctca cgccggagat ggctcggcgt
     1861 ctgaaaattc ccgatacgcg tggaatgctg attacgggcg tcgacccgaa cggcgcgtcg
//
LOCUS       DASWNE010000972         2692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_454070, whole genome shotgun sequence.
ACCESSION   DASWNE010000972 DASWNE010000000
VERSION     DASWNE010000972.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454070"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VGB68_18890"
     CDS             <1..508
                     /locus_tag="VGB68_18890"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY0051370.1"
                     /translation="DAVEEARTLLRTKRKIPPGEKDNFGILTPDAISGIKDRLFGTAS
                     IVILLVPTIALIVGAIVIMNIMLVSVTERTREIGIRKSLGARQSDILKQFLFEAATLS
                     AIGGVIGLLAAELLGVIITAFVFQTRIPLWAAFFAIGVSAAVGILAGLFPAWKAARLD
                     PIEALRAD"
     gene            513..1778
                     /locus_tag="VGB68_18895"
     CDS             513..1778
                     /locus_tag="VGB68_18895"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0051371.1"
                     /translation="MLNFLGSGVYENLKMAIDTLRSNKLRSFLTIFGVLVGVITVMLI
                     SSVISGFDMAIKKEVESFGTRSIFLYKFEIGFTHGNRTREERMRKSLTMDDAEAISKL
                     PTIETAVPFLDISNNFFGQKILVTGKNGKTSSAVNLQGTMPEIERTSSEVLLDGRWFS
                     QTESDMKTNVCLIGSTVAESYFQYGSPIGQTLEIGGSEFRIIGILEKREQLFGGGGGN
                     NDQSNIIYMPMGAALKLKPNADDLFILAVAREGMLQQAQDQVQDLLRIRRQVPFDKPN
                     NFSMATAASIIDQFRAITAGVAIAMIVISSVGLMIGGIGVMNIMLVSVTERTREIGIR
                     KAIGAKQSDILMQFLIEAATLTGLGGLLGLFIGWLLTFLVKMIMPSYVPLWAPIAGFG
                     ASVLIGIVFGLFPAWKAARLDPIESLRYE"
     gene            complement(1878..2678)
                     /locus_tag="VGB68_18900"
     CDS             complement(1878..2678)
                     /locus_tag="VGB68_18900"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HEY0051372.1"
                     /translation="MQEQNPVEWQAPPPPEAIKAEEPPQMSEAATLGNIFFEPGRTFE
                     DLRRKPRFILASVIICLLTTGFLFTFMQKMGEDRYRRFFAEQIDKSSQAGSLSSEQKQ
                     QSVNLQLTFSKYITYILPVLILAGLAMGGLVYWLAGKAMGGSLNYLHGVSVWVYASLP
                     IVVVSILANFIVLFLKSADEIDIAASQRGLISANPSFFIDGKEMPVLATLLGTLDFFQ
                     IWGWILAAIGLQRVARISSGSAWGIVLILALVSLAWRVVQALLTGNPM"
BASE COUNT          636 a          685 c          718 g          653 t
ORIGIN      
        1 cgacgcggtc gaagaagcgc gcacgctttt gcgaacgaaa cgaaagattc cgcccggcga
       61 aaaagataat ttcgggattc tgacgccgga cgccatcagc ggcattaaag accggctttt
      121 cggcacggct tcaatcgtca ttcttttagt tccgaccatc gcgctgattg tcggcgctat
      181 cgtcattatg aatattatgc tggtttccgt cacggaaagg acgcgcgaaa tcggcatccg
      241 caaatcgctc ggcgcgcgcc agtccgatat tctgaaacaa tttcttttcg aggcggcgac
      301 gctttcggca atcggcggcg tcatcggtct gctggcggct gaactgctcg gcgttattat
      361 cacggcgttt gttttccaga cgcgaattcc gctctgggcg gcgttttttg cgattggagt
      421 ttccgccgcg gtcggcattt tagccggact ttttccggcg tggaaggcgg cgcggctcga
      481 cccgatcgag gcgctgcgcg cggattaaat ttatgcttaa tttcctgggt tcaggcgttt
      541 acgaaaactt gaaaatggcg attgatacgc tgcgcagcaa caaactgcgc tcgtttctga
      601 cgattttcgg cgtgctggtc ggcgtgatta ccgtgatgct gatttcgtcg gttatcagcg
      661 gctttgatat ggctatcaaa aaggaagtcg aatcgttcgg cacgcgctcg atttttctat
      721 ataaatttga aatcggcttt acgcacggca accgcacgcg cgaggaaaga atgcgaaaat
      781 ctttaacgat ggacgacgcc gaagcgattt caaagctgcc gaccatcgaa accgccgtgc
      841 cttttctgga tatctccaac aattttttcg gtcagaaaat tctcgtcacc ggcaaaaacg
      901 gaaaaacttc ttcggcggtt aatttgcagg gaacgatgcc ggaaatcgag cggacaagct
      961 cggaggtgct gctcgacggg cgttggtttt cgcagacgga aagcgatatg aaaacgaatg
     1021 tttgtcttat cggctcgacc gtcgccgaaa gttattttca gtacggctcg ccaatcggac
     1081 agacgctgga aatcggcggc agcgagtttc gcattatcgg gattctggaa aaacgcgagc
     1141 agcttttcgg cggcggcggc ggcaataacg accagagcaa cataatttat atgccgatgg
     1201 gcgcggcgct gaaattaaag ccgaatgccg acgatttgtt tattctcgcc gtcgcccgtg
     1261 aaggaatgct ccagcaggcg caagaccagg tgcaggattt gctgcgtatt cgccgccagg
     1321 ttcccttcga caagccgaat aatttcagta tggcgaccgc cgccagcatt atcgaccagt
     1381 ttcgcgcgat aaccgccggc gtggcgattg cgatgatcgt catttcttcg gtcggtttga
     1441 tgatcggcgg catcggcgtg atgaatatta tgctggtttc ggtgacggaa agaacgcgcg
     1501 aaatcggcat ccgcaaagcc atcggagcca agcaaagcga catcctgatg cagtttttga
     1561 ttgaagccgc gaccctgacg ggcttgggcg gcttgctcgg tttgtttatc ggctggctgc
     1621 tgacgtttct ggtaaagatg attatgcctt cgtatgttcc gctttgggcg ccgattgccg
     1681 gattcggcgc gagcgtttta atcggcatcg ttttcggact ttttccggcg tggaaagccg
     1741 cgcggctcga cccgatcgaa tcgcttcgat acgagtaagc aacggataat tgataactga
     1801 taactgataa ctgataattt tcgcaatcaa acattatttt tcagttatca actatcaact
     1861 atcaactatc cgttatttta catcggattt cccgtcagaa gcgcctgcac gactcgccag
     1921 gctaaactga ccagagccaa tattaaaacg attccccagg ctgagccgga agatattctg
     1981 gcgactctct gcagcccgat ggcggctaaa atccagcccc agatttggaa aaaatcaagc
     2041 gtgccgagca gcgttgccag cacgggcatt tctttgccgt caataaaaaa gctcggattg
     2101 gcgctaatca aaccgcgctg gctggcggcg atgtcaatct cgtccgccga cttcaggaac
     2161 aaaacaataa aatttgccag tatggaaacc acgacaatcg gcaaactggc gtaaacccag
     2221 accgaaacgc cgtgcaggta attgagcgag ccgcccatcg cttttccggc gagccagtaa
     2281 accagaccgc ccatcgccag accggcgaga attaaaaccg gcaaaatata agtgatgtat
     2341 tttgaaaagg tcagttgaag attgacgctc tgttgttttt gttccgacga gagcgagccg
     2401 gcttgcgaac tcttgtctat ctgctcggcg aaaaatcggc ggtaacggtc ttcgcccatt
     2461 ttctgcataa aggtaaacaa aaagccggtg gtcaaaaggc atataattac ggacgccaga
     2521 ataaagcgcg gcttgcgccg caaatcctca aaagttctgc ccggctcaaa gaaaatattg
     2581 cctaaagtcg ccgcttcgga catctgcggc ggttcctcgg cttttattgc ttccggaggc
     2641 ggcggcgcct gccactcgac tggattttgt tcttgcataa ttctctcctt ca
//
LOCUS       DASWNE010000973         2360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_316133, whole genome shotgun sequence.
ACCESSION   DASWNE010000973 DASWNE010000000
VERSION     DASWNE010000973.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316133"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="VGB68_18905"
     CDS             complement(<1..665)
                     /locus_tag="VGB68_18905"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0051373.1"
                     /translation="MRHLYSFYLDAFLISVKSILEHKLRAFLTLIGIIIGVAAVVVVG
                     ASINGLKTYVVDKVTKVLGANHFMITRIASTGRMSDEEFEKRNRRNKDITWEEYLYVK
                     ANCLNCSEVGVQSFGGADLSENGVEMPSTRIFGVTDNASEIEDKTIAEGRFLTAEEVT
                     RAAKVCVIGTDVKEKYFPDKSPLGQTVKVRGIPLQIIGVEEKRGSFLGDSMDRHMYIP
                     ITL"
     gene            complement(990..>2360)
                     /locus_tag="VGB68_18910"
     CDS             complement(990..>2360)
                     /locus_tag="VGB68_18910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051374.1"
                     /translation="NVVRLNADGSPDRGFGAGGRTTLNSSPPLFTNLPIPAGSELPAK
                     LLVRENGDIFVAANSAQLVISRRNAPGFLGRAERKFLLAIFRLSPDGRLAGKNFTRVT
                     PAAQAISAYSRFQINGILAQPDGKILTYGSVDFLNFDAPSFGTPDPAIFLAGYTSISA
                     LNDTNNFYDYNFDGRAELAVYRPNSSGLGMWLFLFSDPDGSPAYQSVSREYGMPGDIP
                     APGDYDGDYVQDLAVFRPATGEWITRKIYLNNCAPMDCSEVVQFGASGDIPAPGDFDG
                     DGRTDRAVFRPSIGDWYILLSSGGVRGLHFGQNGDQPVAGDYDGDGKSDAAVIRRENG
                     KMFWYILQSSDNQFVGLQFGLSEDKAVVADYNADAKTDIAVWRPSNGTWYVLADYTNF
                     SAAQFGAPGDVPESFDYDGDRKADFAVFRPSAGVHYILRSSVGDSVGVQFGANGDVPI
                     ASAYVR"
BASE COUNT          566 a          621 c          646 g          527 t
ORIGIN      
        1 tgcagcgtta tcgggatata catatgccgg tccatcgaat cgcctaagaa agagccgcgt
       61 ttttcctcga cgccgataat ctgcagggga attccgcgca ccttgacggt ctgcccgagc
      121 ggcgatttgt cgggaaaata tttttccttg acgtccgttc cgatgacgca gaccttggcg
      181 gcgcgcgtga cttcctcggc ggtcagaaac cttccttcgg caatcgtttt gtcttcgatt
      241 tccgaagcgt tgtcggtgac gccgaagatt cgcgtcgacg gcatctcgac gccgttctcg
      301 ctcaaatccg cgccgccgaa actctgtacg ccgacctccg agcaattcag acaattggct
      361 ttcacataca ggtattcttc ccatgtgatg tccttgtttc ggcgattgcg cttttcgaat
      421 tcctcgtcgc tcattctgcc ggtcgaagcg atgcgggtaa tcataaagtg attcgcgccg
      481 agaacttttg tgactttgtc gacgacgtat gttttcagcc cgttaatcga tgcgccgacg
      541 acgacgaccg ccgcgacgcc gatgatgatg ccgataagcg tcagaaaagc gcgcagctta
      601 tgctccaaaa tagatttgac cgaaatcaaa aaagcgtcca gatagaatga ataaagatgt
      661 cgcataacaa tcgaaaactg cccgtttttt ggaattgacc gcgcttgccg cgctggagat
      721 attgagagtt acgaaatgtt ttcggagaat gtttcggtaa atgcggggat tggaaagttt
      781 tgcccgcgaa agactcgaaa aacgcgaata agaaagaaat tagccgcaga aaacacagag
      841 aaagaattag ccgcagaaaa cacagagaaa gaattaaccg aggataacgg gatttacagg
      901 gctaagaaaa attatttttc ttagccctgc ttatttattt ctctgattcg cgtcttttcg
      961 cgctttttcg cgggcaattt cttcttaaac tacctgacgt aagccgaggc aatcggaacg
     1021 tcgccgtttg cgccgaactg cacgccgacg ctgtcgccga cggaagagcg caaaatgtaa
     1081 tggacgccgg cagacgggcg gaaaacggca aagtcggctt tgcggtcgcc gtcataatca
     1141 aacgactccg gcacatcgcc cggcgcgccg aactgtgcgg cggagaaatt agtgtaatcc
     1201 gccagcacat accaggtgcc gttagacgga cgccagacgg cgatgtccgt tttcgcgtcg
     1261 gcgttataat cggcaacgac ggctttatct tcacttaaac cgaactgtaa gccgacgaat
     1321 tgattgtccg agctttgcag aatataccag aacatctttc cgttttcgcg cctgatgacg
     1381 gcagcgtcgg atttgccgtc gccgtcgtaa tcgcccgcta cgggctggtc gccgttttgc
     1441 ccgaaatgca gcccgcgaac tccgccggaa cttagcagaa tataccaatc gccgatagac
     1501 ggacgaaaga cggcgcggtc tgtcctgccg tcgccgtcaa aatcgccggg cgcgggaata
     1561 tcgccgctcg cgccgaactg aactacctcg gagcaatcca tcggcgcgca gttgttgaga
     1621 taaattttcc gggtaatcca ttcgcccgtc gccggacgaa agacggctaa atcctgaacg
     1681 taatcgccgt cgtaatcgcc gggcgccggt atgtcgccgg gcataccgta ttctcgcgaa
     1741 acagactggt aagcgggaga gccgtcgggg tcgctaaaca aaaacagcca cattccgaga
     1801 ccggaactgt tcgggcgata aacggcgagt tcggctctgc cgtcaaagtt gtaatcgtag
     1861 aaattattcg tatcgttcaa agcggaaatc gaagtatatc cggctaaaaa gattgccggg
     1921 tcgggagtcc cgaagctcgg agcgtcaaaa ttaaggaaat cgaccgagcc gtaagttaag
     1981 attttgccgt cgggctgggc gagaatgccg tttatctgaa accggctata cgccgaaata
     2041 gcttgagcgg caggcgtcac gcgcgtaaaa tttttgcccg cgagccgtcc gtcgggactg
     2101 agccgaaata tggctaaaag gaattttctt tcggcgcgcc cgagaaatcc cggcgcgttt
     2161 ctgcgggaaa taaccagttg tgcgctgttg gcggcgacga aaatatcgcc gttttcccga
     2221 accagaagct ttgccggaag ttcactgccg gctggaatcg gcagatttgt gaagagcggc
     2281 ggcgagctgt tcagagtcgt tctgccgccg gcgccgaagc ctctgtcggg cgagccgtcc
     2341 gcattcagtc gaacgacgtt
//
LOCUS       DASWNE010000974         3598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_280095, whole genome shotgun sequence.
ACCESSION   DASWNE010000974 DASWNE010000000
VERSION     DASWNE010000974.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280095"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1144
                     /gene="metK"
                     /locus_tag="VGB68_18915"
     CDS             <1..1144
                     /gene="metK"
                     /locus_tag="VGB68_18915"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEY0051375.1"
                     /translation="SESVTEGHPDKIADQISDAVLDAILEQDATGRVACETLVTTGLA
                     VIAGEITTNATVNYKKIVRDTIKDIGYDDASYGYDSNTLSVMDAVGTQSPDIAQGVDT
                     GGAGDQGLMFGFACNETPELMPLPIQLAHNLTRRLSEARRSGELPYLRPDGKSQVTIE
                     YRDGLPFRVDAVVISTQHAEEVSTEQLRKDILEKVIKATVSEDLLDENTKYHINPTGR
                     FVIGGPMGDAGLTGRKIIVDTYGGYAPHGGGAFSGKDPTKVDRSAAYMARYIAKNIVA
                     AGLADKCTVQLAYAIGVAEPVSVRIDTHNTGTVDEIRLAELVRENFQLTPKGIIETLD
                     LRRPIYKATAKFGHFGRTNDEFTWEKTDKAEALRTQAETVGAATGN"
     gene            1224..2501
                     /gene="ahcY"
                     /locus_tag="VGB68_18920"
     CDS             1224..2501
                     /gene="ahcY"
                     /locus_tag="VGB68_18920"
                     /inference="COORDINATES: protein motif:HMM:NF004005.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEY0051376.1"
                     /translation="MAKEGISMEYDIKDINLAPQGKQRIEWADREMPVLRLIRERFEQ
                     EKPLEGIKLVACAHITTETANLARTLQAGGAEAILIASNPLSTQDDVAASLVADWNIP
                     VFAHKGESTDTYMRHLRAALETNPNIIIDDGSDVVATMLKEKKELSETIIGTTEETTT
                     GIVRLKAMQKAGVLNFPSIAVNDAQTKHFFDNRYGTGQSTLDGIIRATNILLAGKNFV
                     VVGYGWCGKGCAMRARGMGANVIVTEIDPIKAIEAVMDGFRVLPMTEAAKLGDFFVTV
                     TGNRHVIDKEHFESMKDGAIVCNSGHFDLELNLEALREMSESVENRRPFVEEYMGKNG
                     KSVIVLGEGRLINLAAAEGHPASVMDMSFANQALSAEYLVKQQGKLEAGVHVLPPEVD
                     QEIASLKLRSLGISIDTLTPEMLEYMSSWETGT"
     gene            complement(2593..>3598)
                     /locus_tag="VGB68_18925"
     CDS             complement(2593..>3598)
                     /locus_tag="VGB68_18925"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEY0051377.1"
                     /translation="IYIIFTAFEMWRFAGTIDNGFSLLTSYLTYLLPHIYIQIAPSCL
                     MIATLATFVIKSRQNEVVTWTAAGQSVYRLLLPCFLLMAAIGLVNWQVQERVLPKTNR
                     IQDTLRTQIRNKGVLAAKEGKFWIASDNRVYSFDLNEKDFERQRVTNLYVFEFAEDGE
                     KLKSFIKSEEAIWSREKIELTGTAQKTLWQENSRIENKPLAATERALEASFNPFRRMY
                     TKPNQLTASETAEKLESSESESEKRTYAVALEKKYTTIVLPFIITLFTAPFALSLSRK
                     GKALTVGYAVGIWLLFMGITSTFEQFGLNGYVAPSIAVWSPLVLFTIIGIYLITRVKT
                     "
BASE COUNT          907 a          967 c          940 g          784 t
ORIGIN      
        1 ttccgaatcg gtcaccgaag gtcatcccga taaaatcgcc gaccagattt ccgatgccgt
       61 cctggacgcg attctggaac aggacgcgac cggcagagtc gcctgcgaaa ctttagtcac
      121 gacgggtctc gccgtcatcg ccggtgaaat taccactaac gccacggtta attataaaaa
      181 aatcgttcgc gacacgatta aggacatcgg ttacgacgat gccagctacg gctatgacag
      241 caacactcta agcgttatgg acgccgtcgg cacgcagtcg ccggacatcg cgcagggcgt
      301 ggatacgggc ggcgccggcg accagggatt gatgttcggt tttgcctgta acgaaacgcc
      361 ggaattgatg cctttgccga ttcagttggc gcataacctg acgcgcaggc tttccgaagc
      421 gcggcgcagc ggcgagcttc cctatctgcg tcctgacggc aagtcgcagg tgacaatcga
      481 ataccgcgac ggtctgccgt tccgcgtcga tgccgtcgtt atctcgacgc agcacgccga
      541 ggaagtgagc accgagcagc tccgaaagga tattctggag aaagtcatca aagcgaccgt
      601 ctccgaagac cttctggatg aaaacaccaa atatcacatc aatccgaccg gacgcttcgt
      661 tatcggcggt ccgatgggcg acgcaggctt gaccggacga aaaatcatcg tcgatacata
      721 cggcggctac gctccgcacg gcggcggcgc tttctccggc aaagacccga caaaggtcga
      781 ccgctcggcg gcttatatgg ctcgctacat cgccaaaaac attgttgccg cgggcttggc
      841 tgacaaatgc acggtacagt tggcatatgc catcggcgtc gccgagccgg tttcggttcg
      901 gattgacacg cacaatacgg gaacggttga cgaaataaga ctcgccgaac tcgtccgcga
      961 gaatttccag ttaacgccca aaggcattat cgaaacgctc gatttgcgcc gcccgattta
     1021 caaggcgacc gccaaattcg gacatttcgg acgcaccaac gacgagttca catgggaaaa
     1081 aaccgataaa gccgaagctt taagaacgca agccgaaacc gtcggcgcgg caaccggaaa
     1141 ttaatttgct tttgagcggt aagcatttgt ttaagaacaa cttgccgctc tttttctatt
     1201 taaaatcatt acaggaagaa tttatggcta aagaaggaat ttcgatggaa tacgatataa
     1261 aagatattaa tctcgccccg cagggcaaac aaagaatcga gtgggctgac cgcgaaatgc
     1321 cggttctgcg cttgattcgc gagcgtttcg agcaggaaaa accgctggaa ggaatcaagc
     1381 tcgtcgcctg cgcgcacatt acgaccgaaa ccgccaatct ggcgcgcacg ctgcaggcag
     1441 gcggcgcgga agcaatttta atcgcttcga acccgctctc gacgcaggac gatgtcgcgg
     1501 cttcgctcgt cgccgactgg aacattccgg ttttcgcgca caaaggcgaa tcgaccgata
     1561 cgtatatgcg tcatctccgc gcggcgcttg aaacaaatcc gaacatcatt atcgacgacg
     1621 gctcggacgt ggtggcgacg atgctcaagg aaaagaaaga gctttccgaa acaatcatcg
     1681 gcacgaccga agaaacgacg accggcatcg ttcgcctgaa agcgatgcag aaagccggcg
     1741 tgctgaactt cccttcaatc gccgtcaacg acgcgcaaac caaacatttc tttgataacc
     1801 gctacggcac tggacaatcg acgctcgacg gaattatccg cgcgaccaac attctgctcg
     1861 ccggaaaaaa ctttgtcgtc gtcggttacg gctggtgcgg caaaggctgc gcgatgcgcg
     1921 cgcgcggaat gggcgcaaac gttatcgtca ccgaaatcga cccgattaaa gccatcgaag
     1981 ccgtgatgga cggtttccgc gttctgccga tgacggaagc cgccaagctc ggcgatttct
     2041 tcgtcaccgt gaccggcaat cgccacgtta tcgacaagga acatttcgaa tcgatgaaag
     2101 acggcgcgat tgtctgcaac agcggtcact tcgatttgga attgaatctg gaagctcttc
     2161 gcgaaatgtc tgaatcggtc gaaaaccgcc gcccgtttgt cgaagaatat atgggcaaaa
     2221 acggcaagag cgttatcgtc ctcggcgaag gtcgcctgat taatctggcg gcggcggaag
     2281 ggcatccggc ttcggtgatg gatatgagct tcgccaacca ggcgctctcg gctgaatatc
     2341 tggtcaagca gcagggcaag ctcgaagcgg gcgtgcacgt tctgccgccc gaagtcgacc
     2401 aggaaatcgc cagcctgaag ctccgctcgc tcggcatttc aatcgatacc ttaacgccgg
     2461 aaatgctcga atatatgtcg agctgggaaa ccggaactta ataaaaccta gcggaaaacg
     2521 gaaaacggta agcggtaagt tgcagttata aaataacgaa cttaccgctt accgttttcc
     2581 gtttatcgct ttttacgttt taactcttgt aatcagataa ataccgataa tcgtaaataa
     2641 aacgagcggg ctccagacgg cgattgaagg cgcgacgtaa ccgtttaatc cgaactgctc
     2701 gaaagtgctg gtaatgccca taaaaagaag ccagatgccg accgcgtaac cgaccgtcaa
     2761 agcctttcct ttccggctga gcgaaagcgc aaacggcgcg gtaaacagag tgatgataaa
     2821 cggcagaacg atggtcgtat attttttctc cagcgcgacc gcatacgttc tcttttcgct
     2881 ttccgattcc gagctttcga gtttttcggc ggtttcggac gcggttaatt gattcggctt
     2941 tgtgtacatc cggcgaaacg gattaaacga tgcctccaaa gctctttccg tcgccgccag
     3001 cggtttgttt tcgatacgac tgttttcctg ccaaagggtt ttctgcgccg ttcccgtcaa
     3061 ttcgattttt tcccgcgacc agattgcctc ttcggatttt ataaacgatt tcagcttttc
     3121 gccgtcttcc gcaaactcga aaacgtataa attagtgact ctctgtcgtt caaaatcctt
     3181 ctcgttcaga tcgaacgaat aaactcgatt atcggacgca atccagaact ttccttcctt
     3241 ggctgcgaga actcccttgt tgcggatttg cgtgcgcaga gtgtcctgta tgcggttggt
     3301 tttcggcaga actcgctcct gaacctgcca gtttactaat ccgattgccg ccatcaatag
     3361 aaaacagggc agcaaaagac gatagacgct ctgcccggca gccgtccacg tgacgacctc
     3421 gttctgtctg gatttgatga cgaaagtcgc cagcgtcgca atcatcaggc acgacggcgc
     3481 gatttgaatg taaatgtgcg gaagcagata tgtgagatag ctcgttaaaa gactgaatcc
     3541 gttgtcgata gttcctgcga atcgccacat ctcgaaggct gtgaagataa tgtaaatc
//
LOCUS       DASWNE010000975         4411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_316216, whole genome shotgun sequence.
ACCESSION   DASWNE010000975 DASWNE010000000
VERSION     DASWNE010000975.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316216"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VGB68_18930"
     CDS             <1..465
                     /locus_tag="VGB68_18930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051378.1"
                     /translation="DVVIDSPTPEAIDSPLLENIEQAKTKPVAIEKTVAKKENNDDPA
                     PKTEPAEKIENPEKPKPAQVEVTRPVQTPRRSEPQRRNQTPARSAESQPVPDIESIFT
                     GRPSDRREERPRRQEQQRRQPPEDMSEEEWQEWRRQRREQRRRRQLRNTFPF"
     gene            complement(676..1740)
                     /locus_tag="VGB68_18935"
     CDS             complement(676..1740)
                     /locus_tag="VGB68_18935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051379.1"
                     /translation="MILKAARFLIILTVSIICFGETLYAQGMRDRISGNVCQSSSGLN
                     GVYRIDVDGSDKLYSVIEGATSTVPYGEQQQFFTDLAVRLTPPDLLAIECRGNRVSLG
                     SSRAARVDFVADGITRSIRTKDGGVLRSRIGLERSSLTFISSGGTNDSVNFTFTPLEN
                     GRRLRVTRRISAAELIEPVIIQTFYNKINEVVSWDIYAAEPLDNRVAGQTNEKTPTTI
                     NTRTRISGGGNNEANSLREALARWVDATNARNIEKQMTFYMPELKAFYLARNASKSSV
                     RIEKTRTFAAAKSIDVRAEEPEIIFQNGGRMAIMRFRKKYNIENGAKSRRGEVVQELR
                     WQQTAGGWKIFSERDIKVLR"
     gene            2029..2262
                     /locus_tag="VGB68_18940"
     CDS             2029..2262
                     /locus_tag="VGB68_18940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051380.1"
                     /translation="MRKKKIGCFRLSGGLSRHRHLCESLRIESLSPKYKRVNAKVAVI
                     AAPAVGDFGVFSLENSSGKLNARARKPSAHLLP"
     gene            2317..2754
                     /locus_tag="VGB68_18945"
     CDS             2317..2754
                     /locus_tag="VGB68_18945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051381.1"
                     /translation="MYCNQKYSGVVKGFLLGAVLVFSVMFLGSNSNKYQSTKNAVSFE
                     ESLSRIKSDDIKEVRIKNETAEFFKDGQLAFSTSVDSDFMKEALLGQISSNRISVSFA
                     PESSNPLGYLIQILFWLFFISPPIIVVQLLVIIKKMDASSSNR"
     gene            2832..3341
                     /locus_tag="VGB68_18950"
     CDS             2832..3341
                     /locus_tag="VGB68_18950"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY0051382.1"
                     /translation="MNVSIRQLTGDDWREFSEVRLKALETDPLVFGSNYEKESQFTET
                     DWRSRLQANDSAVFMLFAGETPIGITGVSVFRDDPTGKTALLWGSWLEPNFRGKGLSE
                     LMYKARLDWAKARATLEKIIVSHRASNLASKYANQKHGFTFTGTHEKLWSDGATEDDV
                     CYELYLKPE"
     gene            complement(3697..>4411)
                     /locus_tag="VGB68_18955"
     CDS             complement(3697..>4411)
                     /locus_tag="VGB68_18955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051383.1"
                     /translation="RVFPYHLLVAYTAASQNRDIPREITEALQDAMEIATENVPEIKG
                     KVYVFPDISGSMHSAVTGYRKGSTSAVRCLDVAALVAAAILRKNPSAEVVPFSDHIVQ
                     ADLNPRDSVMTNAQKLAKLPSGGTNCSAPLFSLNAKKAKGDVVIYVSDNESWVDKTRS
                     HSGTQTMIQWNEFKSRNADAKLVCIDIQPYAHTQAGERADILNVGGFSDQVFTLVSEF
                     AAGTLDAGHWVGVIEKVEI"
BASE COUNT         1111 a         1130 c         1091 g         1079 t
ORIGIN      
        1 gacgtcgtta tagattcgcc gacgcccgaa gcgattgatt cgcctttgct ggaaaatata
       61 gagcaggcta aaaccaaacc cgtggcaatc gaaaaaactg tcgccaagaa agaaaataat
      121 gacgatccgg ctcctaaaac agagcctgcc gaaaaaatag agaacccgga aaaaccgaag
      181 cccgctcagg ttgaagttac gcgtccggtc caaaccccgc gccgcagcga gccgcagcga
      241 cgcaatcaga ctccggcgcg cagcgccgaa tcacagccgg ttccggacat cgaatcgatt
      301 tttaccggtc gcccttcgga tcggcgcgaa gaacgaccac ggcgtcagga acaacagcgc
      361 cgccagccgc ccgaagatat gagcgaagaa gaatggcagg aatggcgtcg gcagcgccgg
      421 gaacagcgtc ggcgacgtca actcagaaac acttttccgt tttaaggaag attcgagcga
      481 ttctttcggt agaatagatg gacgataatt tatctcccgt ctattttttt ttgaaaaatt
      541 attcgagaat aaagtgaatt tcagataaat cacgggagat ttcggaataa ctgaaaaccg
      601 aaaactgaaa gtttttatcc gtcagctatc ggatttgagt tattccgaat ccgtttccat
      661 tgtttgaaac cggctttagc gcaaaacctt gatgtcgcgc tcgctgaaaa tcttccatcc
      721 gccggcggtt tgctgccagc gcagctcctg gacgacctcg ccgcggcggc ttttcgcgcc
      781 gttttcgata ttgtattttt tgcgaaaacg cattatcgcc atccgcccgc cgttctgaaa
      841 tattatttcc ggttcttcgg cgcgcacgtc gatggattta gccgccgcga aagtacgggt
      901 tttttcgatg cggactgagc ttttggaagc attgcgggcg agataaaacg cttttaattc
      961 gggcatataa aaggtcattt gtttttcgat atttcgcgca ttcgtcgcat cgacccagcg
     1021 cgctaaagct tcgcgcaggg aattggcttc gttatttccg ccgcccgaaa tccgcgtgcg
     1081 cgtgttaata gtcgtcggag tcttttcgtt tgtttgtccg gcgacgcggt tgtccagcgg
     1141 ttccgcggcg taaatatccc agctgacgac ttcattaatt ttgttgtaga aggtttgaat
     1201 aataaccggc tcgataagct cggcggcgga aatgcggcgc gtcacgcgca ggcgtctgcc
     1261 gttttcgagc ggcgtaaaag tgaaattcac gctgtcgttg gttccgccgc tggaaataaa
     1321 ggtcagactg ctgcgctcca gcccgatgcg cgagcgcagc acgccgccgt ccttcgtgcg
     1381 tatgctgcgg gtaattccgt cggcgacaaa atcgacgcgc gccgctcgtg aagaaccgag
     1441 cgaaacgcgg ttgccgcgac actctatcgc cagaaggtca ggcggcgtca ggcgcaccgc
     1501 caaatcggta aaaaactgct gctgctcgcc gtacggaaca gtgctcgtcg cgccttcgat
     1561 aacggaataa agcttatcgc tgccgtcgac gtcgattcgg taaactccgt taagcccgga
     1621 cgacgactgg cagacgttcc cgcttattct atcccgcatt ccctgcgcat aaagcgtttc
     1681 gccgaagcag atgattgaaa ctgtcagaat tattaaaaag cgcgcggctt ttagtatcat
     1741 ttcgccccct tgttaatttc attacactgt ttttgatagc tgtattatac gccgaattcg
     1801 acaaatttta tacaaaaaaa taatgccgtc cgggcatcca gccgggattt taaattcaag
     1861 ggctttttgc agccttaggt tgtgaaaaaa ggacgacaag aattaaaacg attcacagtc
     1921 ggcaggactc gaacctgctt ctccggctcg cgccggcgct ttactcctta agctacatcc
     1981 tattctggtt tggaatgtaa tcgttttggc attcgtcccg taaacgatgt gcgaaaaaaa
     2041 aagatcggct gctttcggct ttcaggcggt ctcagccggc atcggcattt atgcgaaagt
     2101 ttacgaatcg aaagtctctc gccgaaatac aaaagggtca acgccaaagt cgccgtgatt
     2161 gccgcgcccg cggtcggcga ctttggcgtt ttttcgctgg aaaattcgtc cggtaaatta
     2221 aatgcgcggg cgcgaaaacc gtccgcgcat ttattgccgt aaaatccttt ttcaaaattc
     2281 cccgatgtgt tacgattgcg gcattggagg aaaattatgt attgcaatca aaaatattcc
     2341 ggcgtcgtca aaggttttct gctcggagcc gttttagttt tttcggtgat gtttctcggc
     2401 tcgaattcga acaaatatca atcgacaaaa aacgcggtct cttttgaaga atcattatca
     2461 cgcattaaat cggatgatat aaaagaagtc agaatcaaaa acgaaacggc tgagtttttt
     2521 aaagacggtc agctcgcttt ctcgaccagc gtcgattccg actttatgaa ggaagctctg
     2581 ctcgggcaaa taagctcaaa tcggataagc gtcagcttcg cgcccgaatc ttcaaacccg
     2641 ctcggctacc tgattcaaat cctgttctgg ctgtttttta tcagcccgcc gatcatcgtc
     2701 gttcagctcc tggtaatcat caaaaaaatg gacgccagca gttcaaacag gtaaaacgga
     2761 gggcaagaga aatcttgtcc ttttctgctg cactacaaac tttttctctt ccgtcggcga
     2821 cgggaaaatt gatgaatgtt tcgattagac aattaaccgg agacgactgg cgcgagttct
     2881 ccgaggttcg cttgaaagcg ctcgaaaccg acccgctcgt tttcggctca aactacgaaa
     2941 aggaatcgca atttaccgaa accgattggc gcagccgtct gcaagcaaac gattccgccg
     3001 tttttatgct ttttgcgggc gaaacgccaa tcggaataac cggcgtttcc gtctttcgcg
     3061 acgacccgac aggcaaaacc gctcttttgt ggggctcctg gttagagccg aattttcgcg
     3121 gaaaaggctt atccgagcta atgtataaag cgcgcctcga ctgggcgaaa gcgcgcgcga
     3181 cccttgaaaa aataatcgtc tcgcaccgcg cctctaatct cgcctcaaaa tacgccaatc
     3241 agaaacacgg cttcacgttc accggcacgc acgaaaaact ctggtctgac ggcgcgaccg
     3301 aagacgatgt ctgctacgag ctgtatctca aacccgaata acaaacagcc atccaaatct
     3361 aaaaattggc gacaagttat gccgagagtt ccgcccgtca aacaaaatcg ggcgggaaag
     3421 aatcgaactt tctaaaccaa tgtactcacg agcggcattc gccaaataaa cgaagaatta
     3481 tttaatttaa ccggttttag ggataaaacc tttatataaa ggtttcatat ttttgatgaa
     3541 attcttacaa aaaataataa tatttgaaat aaagctattt gccgaatatt cattattatt
     3601 ttgaatcccg ctggaaatct caatccgtcg gaaggcagtt atcaggtaag aattgcagat
     3661 gaaaacaaat taattcattt tcatctgcaa tcctcattaa atctcgacct tctcaatcac
     3721 gccgacccag tgtccggcgt ccagtgttcc ggcggcgaat tccgagacga gcgtaaagac
     3781 ctggtcggag aatccgccga cgttcaggat gtcggcgcgt tcgccggctt gcgtgtgggc
     3841 gtaaggctgg atgtcgatgc agacgagttt ggcgtccgcg ttgcgagact tgaactcgtt
     3901 ccattgaatc atcgtctgcg ttccgctgtg cgaacgggtt ttatcgaccc acgattcgtt
     3961 gtcggaaacg taaatcacca cgtcgccttt tgcttttttc gcattcagcg agaagagcgg
     4021 cgcggagcag ttggttccgc ccgaaggcag tttcgcgagc ttctgcgcgt tggtcataac
     4081 cgaatcgcgc gggttcaaat ccgcctgcac gatgtggtcg ctgaacggga cgacttccgc
     4141 cgaaggattt ttgcgaagaa tcgccgccgc gaccagcgcc gctacgtcaa gacatctgac
     4201 ggcagaagtc gagcctttgc ggtagccggt caccgccgag tgcatcgagc ccgaaatatc
     4261 cgggaaaacg taaactttcc ctttgatttc cggcacgttt tcggtagcga tttccatcgc
     4321 gtcctgcaat gcttcggtga tttcacgcgg gatgtcgcga ttttgcgaag ccgccgtgta
     4381 cgcgacgagc agatggtacg gaaacacccg c
//
LOCUS       DASWNE010000976         1859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_67474, whole genome shotgun sequence.
ACCESSION   DASWNE010000976 DASWNE010000000
VERSION     DASWNE010000976.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67474"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..>1859)
                     /locus_tag="VGB68_18960"
     CDS             complement(38..>1859)
                     /locus_tag="VGB68_18960"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0051384.1"
                     /translation="VLYGYLADVHWKLEERQDALAAARKAVSIEPEYEWAWHAIKSWA
                     EKLDQPNLAAELARDLTAKKPKDVRAWLTCAGILDTDGTHFSQEQMDAIEQALKLEPQ
                     HVTALAMKADALADAHRFDEAIAVCQTKFSDGYRHEQLRYVQAGIEATRGNFEESIRQ
                     LEELAVTNPDYYPAWERLASIYSEWAEKKSDYLRVTREMTRLAPQNSVTFGYLGEACL
                     LNEKRDEAKKAFQQSINLTPEYEFGGMSLFDLYFEDSELQNCKAVLKTLKEFVKGDNT
                     LIREIAFYAKQNDLKNIEPLWKQLCFSETAKDFHFDYALEKLRRFKLINTPFVYKTLS
                     ESCADASANPLVGAYLIELCGERESVENCRKTLEGLRENEKVWTQAMKKFLNVMLQTK
                     SKPDVYSFIKKNWNELKKNDEVWALTGYALNAMEQDKKASEWFSDWRERKTVLPWMLW
                     NYVISLRRLGKAEEASLISRSALNLEKDAIVSLHRMMLGLDELHAGKFENAAQFFNQI
                     DTRVLDDWDRYFYYLLETGLSIYEKNAGGLTAEADELTREFVKNSLAQGNCWTDKMRL
                     TSFRRTLRTALSMSGSGWLKFYLHGRVTLSWLRFQINKSY"
BASE COUNT          391 a          519 c          455 g          494 t
ORIGIN      
        1 tacagtttat ggaaaaaccc ggaaagcggc ttgcccgtca gtaagatttg tttatctgaa
       61 aacgcagcca cgaaagcgtg acccgaccgt gcagataaaa tttcagccag ccgctgccgc
      121 tcatcgacaa agccgttcgc agagttcgcc gaaagctcgt gagcctcatt ttatccgtcc
      181 agcaatttcc ctgcgccaga gagtttttaa cgaattccct ggtcagctcg tcggcttcag
      241 ccgtcaagcc gcccgcgttt ttttcataaa tcgacaagcc cgtctcgagc agataataaa
      301 aatatctgtc ccagtcgtcc agcactctgg tatcgatttg attaaaaaac tgcgctgcgt
      361 tttcaaattt tccggcgtgc agctcgtcca gcccgagcat catccggtgg aggctgacaa
      421 tggcgtcttt ttccagattc aaagcgctgc ggctgatcaa gctcgcttct tcggctttgc
      481 ccagacgccg gagcgaaatc acgtaattcc agagcatcca cggcaaaacc gtttttcgct
      541 cgcgccagtc ggaaaaccat tcggaggctt ttttatcctg ctccatcgcg ttcagcgcgt
      601 agccggtcag cgcccacact tcgtcgtttt ttttcagctc gttccaattc tttttaataa
      661 aggaataaac gtccggtttc gatttggtct gaagcataac gttgagaaat tttttcatcg
      721 cctgcgtcca gactttttcg ttttcgcgca agccttcgag ggttttccgg cagttttcga
      781 cgctttccct ttcgccgcac aattcgatta aataagcgcc gaccagcgga ttggcgctcg
      841 cgtccgcgca gctttcactc aaggttttgt aaacgaacgg cgtatttatc aatttaaaac
      901 ggcgaagttt ttccagcgcg taatcgaaat gaaaatcctt cgccgtctcg gaaaaacaga
      961 gctgtttcca gagcggctcg atgtttttca aatcgttctg cttggcgtaa aaagcgattt
     1021 cacgaatcag cgtattgtcg cctttgacaa actctttgag agtcttcaga acggctttgc
     1081 agttttgcag ctcgctgtct tcaaaataca aatcaaaaag cgacataccg ccgaattcgt
     1141 attcgggcgt cagattgata gactgctgaa aggctttttt ggcttcgtcg cgtttctcgt
     1201 ttaaaagaca ggcttcgccc agataaccga aagtgaccga gttttgcggc gcgagtctgg
     1261 tcatttcgcg cgtcacgcgc aggtagtcgg atttcttttc cgcccattcg ctgtagatgc
     1321 tcgccaggcg ttcccaagcc gggtaataat ccggattagt gacggcgagc tcctcgagct
     1381 gcctgatgga ttcctcgaaa tttccgcgcg tcgcttcgat tccggcttgc acgtaacgca
     1441 attgctcgtg acggtaaccg tcgctgaatt tcgtctggca gacggcgatt gcttcgtcga
     1501 aacgatgcgc gtcggcgagc gcgtcggctt tcatcgccag cgccgtcacg tgctgcggct
     1561 caagctttaa ggcttgctcg atggcgtcca tctgctcctg cgaaaaatgc gtcccgtcgg
     1621 tgtcgagaat tcccgcgcag gtcagccagg cgcggacgtc tttcggtttt ttagcggtca
     1681 aatcacgcgc cagctcggcg gcgagattcg gctggtctaa tttttccgcc cagcttttga
     1741 tggcgtgcca agcccattcg tattccggct cgatcgaaac ggcttttcgc gccgccgcca
     1801 gcgcgtcctg cctttcctcc agcttccagt ggacgtccgc cagataaccg tataaaacg
//
LOCUS       DASWNE010000977         1613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_316261, whole genome shotgun sequence.
ACCESSION   DASWNE010000977 DASWNE010000000
VERSION     DASWNE010000977.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316261"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /locus_tag="VGB68_18965"
     CDS             complement(<1..1295)
                     /locus_tag="VGB68_18965"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HEY0051385.1"
                     /translation="MRDTIQSLPLVQIQRSAAAASFSMPQKQASFHLFDTETGKHILL
                     VNGSRIFTIPDEIAAALDGNPPDSGKLLEKLGLSAPDYITDEPVSNAPVRSLSLAIAQ
                     KCNLGCTYCYAQEGNFGSKNKDMSAEVATASVNLLFSEVTEGEIVNLAFLGGEPLTNR
                     KLLRECTTRAVALAESKNVPVNFSITTNGTLVTPEDGAFFERYKFAVTISLDGIGEVH
                     NRLRAFKSGSGSYRRILENVRPLLAMQRDMQVSARVTVTPDNLFLREALDEFIRLGFH
                     SVGFSPMLSSPSGTGEMNSNQLDVMLEQMISCGEEFERRVINGERFPFLNMANALREI
                     HKGTHRPYPCGAGAGYLGVSADGGLYACHRFVEDEEGAMGKVSDGVDREKQKRWLADR
                     HVHFQEPCRSCWARYLCGGGCHHEVIKRGRPACNYIRGWL"
     gene            complement(1413..>1613)
                     /locus_tag="VGB68_18970"
     CDS             complement(1413..>1613)
                     /locus_tag="VGB68_18970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051386.1"
                     /translation="RPDGTSAGNNWMQKDRTPETPKVYMVDDWMDPNLVNYTDLFRDW
                     ENSLRFIIGGKDEEPPPKQEKE"
BASE COUNT          375 a          451 c          403 g          384 t
ORIGIN      
        1 agccagcccc gaatataatt gcaggcgggt cgcccgcgtt taattacttc gtgatgacaa
       61 ccgccgccgc acagatagcg cgcccagcag ctgcggcacg gctcctgaaa atgcacgtga
      121 cgatcggcaa gccaacgctt ctgtttttcg cggtcgactc cgtccgaaac ttttcccatc
      181 gcgccttctt catcttcgac aaaacgatga caggcgtata aaccgccgtc cgccgaaacg
      241 cccagataac ccgcgcccgc gccgcacgga tacggtcgat gcgtgccttt gtgaatttcg
      301 cgcagggcgt tcgccatatt caaaaaagga aatctctcgc cgtttataac gcgccgctca
      361 aattcctcgc cgcaggaaat catctgttcg agcatcacat ccagttgatt cgaattcatt
      421 tcgccggttc cgctcggcga ggacaacatc ggcgaaaaac cgacgctgtg aaatccgagc
      481 cggataaatt cgtcgagcgc ttcgcgcaaa aacaaattgt cgggcgtaac cgtcacgcgc
      541 gccgaaacct gcatatcgcg ctgcattgcc agcagcgggc gaacgttttc cagaatgcgc
      601 cggtagcttc cgctgccgct tttaaacgcc cgcagtcgat tatgcacctc gccgattccg
      661 tccagactga tggtgacggc gaatttatat cgctcaaaaa acgcgccgtc ttcgggcgtc
      721 actaaagtgc cgttggtcgt aatcgaaaaa ttgacgggaa cgttttttga ttcggctaaa
      781 gcgacggcgc gcgtcgtaca ttcgcgtaaa agctttcgat tcgtcaaagg ctcgccgccg
      841 agaaacgcga gattaacgat ttctccttcc gtcacctccg aaaacagcag gttcacggac
      901 gccgtagcga cttccgcgct catatcttta tttttcgagc cgaaatttcc ctcctgcgcg
      961 tagcagtatg tacagccgag attacatttc tgcgcgatgg cgagcgaaag agagcgaacc
     1021 ggagcatttg acacaggctc gtccgtaata taatccggag cggaaagacc gagtttttcg
     1081 agtaattttc cggagtcggg cggatttccg tcgagcgccg cggcgatttc gtcgggaatc
     1141 gtaaagatac gcgagccgtt gacgagcaga atgtgtttgc ctgtttccgt atcgaaaaga
     1201 tgaaagcttg cctgcttttg cggcatcgaa aaagatgcgg cggcggcgct tcgctgaatc
     1261 tggactagcg gcaaactttg aatagtatct ctcataaaaa ttttcgcccg gcaaaacagc
     1321 ctcaatcaag cgaaaaataa gcgttaaaac aattggtttt tctttcaagt ttcaggcaaa
     1381 gttttcgatt gtccgctctt ttataataaa tttcactctt tttcctgttt cggcggcggt
     1441 tcctcgtcct tgccgccgat gataaagcgc agcgaattct cccagtcgcg aaacaaatcg
     1501 gtgtagttta ccagattcgg gtccatccag tcatcgacca tataaacctt gggagtctcc
     1561 ggcgtgcggt ctttctgcat ccagttattg cccgcgctcg tgccgtcggg acg
//
LOCUS       DASWNE010000978         3285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_136725, whole genome shotgun sequence.
ACCESSION   DASWNE010000978 DASWNE010000000
VERSION     DASWNE010000978.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136725"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            352..1584
                     /gene="ilvA"
                     /locus_tag="VGB68_18975"
     CDS             352..1584
                     /gene="ilvA"
                     /locus_tag="VGB68_18975"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEY0051387.1"
                     /translation="MTVKLSDIQDARKILQGIIIPTPILSDEKLSKEIGAQAFLKAEC
                     LQRSGSFKVRGAYNKISRLSKEEKKRGVVTASAGNHAQGVALAARLHNIKATIVLPEF
                     APLTKIMATKAQGAEVVLKGASFDEAVAYSRELQEKHGYIYVHAFDDDLVIAGQGTIG
                     WEIVEDLPEATVVVVPIGGGGIISGIALAVKNLLPKVRVIGVQAENIAPVRRSLLEGK
                     PVAVEAAQTIADGIAVKRPGEKTLQIIQQFVDEVVEVTEEEIARGIFHCVQNNRLVVE
                     GAGAAGIAALLAKKIKVKPDDTVCTVLCGGNIDGNLLARVIEQVMVRQGRYIILKLLV
                     LDRPGMLAALLNHVAESGANVVEVFHRRAMWLAPLGRVGIEMVLEVKDEKHGREVNKH
                     LEKCGYHVEREGVGDWEE"
     gene            2093..2782
                     /locus_tag="VGB68_18980"
     CDS             2093..2782
                     /locus_tag="VGB68_18980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051388.1"
                     /translation="MTDKEISEFNSAVRMIDFNAANAAAFAGNAKMTGGFAALASEVE
                     VLESAGANRVSARGIRTDGTLDKNAAKTDLLALLRKIAETAKTIKKDEPAFENTFKLP
                     RGTLGSQQLLDTASSFAAMLTAPLIARFGEFGLASATPANLNDKIAAFQTARAQQNAG
                     KSVGVASTAETKASIKRLMQARRTTAQIGENILEDSGDAGLLEAWRSACRIERHKTKP
                     AENPPPQPPTP"
     gene            2926..>3285
                     /locus_tag="VGB68_18985"
     CDS             2926..>3285
                     /locus_tag="VGB68_18985"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEY0051389.1"
                     /translation="MANYQEIITIDPQDAVPRIRHLKISVQQILDLISKGITEQQILE
                     LHRALTLEDINACRVYAAVNPKRENSLDKIIGDLENTSKRLNWGGYILFTISIILGIV
                     LYLDVRFNTISHIDNYLT"
BASE COUNT          905 a          865 c          813 g          702 t
ORIGIN      
        1 aagtttttac gcattagcaa gtggaggcga aaaaaacgcc atgttgttaa aagttatttc
       61 aaaacagaat cgaaacgcga aaagcattca gttttcaaat cattatccgc atatatccgc
      121 atttatccgc ttaattcatt cctaaaaact tttcgcgttt cggtttttct tttttccgaa
      181 tcgaacaata cgtaaaatca gaaacgcgcg cgttcaccga gcgtgttttt tatttaatga
      241 cggaagcgcg ctcgccgaaa cgcccgcgtt ttcgcccgtc tcgcataaat ctcaaatttc
      301 ctctactaaa agctacaatc aaacagtttt tcggcgtata atccaaaaaa tatgaccgtc
      361 aaactttccg atattcaaga cgcccgaaaa attctgcagg gcattattat cccgactccg
      421 attctctcgg acgaaaagct ctccaaggaa atcggcgcgc aggcgtttct gaaagccgag
      481 tgcctgcagc gcagcggctc cttcaaagtg cgcggcgcgt ataacaaaat ctcgcggctt
      541 tccaaagaag aaaaaaaacg cggcgtcgtc accgccagcg ccggcaatca cgcgcagggc
      601 gtggcgctcg ccgcccgcct gcacaacatt aaagcgacga ttgttttgcc ggaattcgcg
      661 cctttaacga aaattatggc gaccaaagcg cagggcgcgg aagtggtttt aaagggcgcg
      721 agctttgacg aagccgtcgc ctattcgcgg gagcttcagg agaaacacgg ctatatctac
      781 gttcacgctt tcgacgacga cctcgtcatc gccgggcagg gaacaatcgg ctgggaaatc
      841 gtcgaagatt tgccggaagc gaccgtcgtg gtcgtgccca tcggcggcgg cgggattatt
      901 tcgggcatcg cgcttgccgt gaaaaatctt ttgccgaagg tgcgcgtcat cggcgtgcag
      961 gcggaaaaca tcgcgcccgt tcgtcgttcg ctgctcgaag gaaaacccgt ggcggtcgaa
     1021 gcggcgcaga ccatcgccga cggaatcgcc gtcaagcgcc cgggcgaaaa aactctacag
     1081 attatccagc aatttgtcga cgaagtcgtc gaagtcacgg aagaagaaat cgcgcgcggg
     1141 atttttcact gcgtccagaa caatcgtctg gtcgtcgaag gcgcgggcgc ggcgggcatc
     1201 gcggcgctgc tcgccaaaaa aatcaaagtc aagccggacg acacggtgtg cacggttctc
     1261 tgcggcggaa atatcgacgg caaccttctg gcgcgcgtga tagaacaagt aatggtcagg
     1321 cagggacgct atattatttt aaaactgctc gtcctcgacc gccccgggat gctcgccgcc
     1381 ctgctcaatc acgtcgccga aagcggagcc aacgtcgtcg aagtcttcca ccgccgcgcc
     1441 atgtggctcg cgcctctggg cagagtcgga atcgagatgg ttctcgaagt caaagacgaa
     1501 aaacacgggc gcgaagtcaa caaacatctc gaaaaatgcg gctaccacgt cgaacgcgaa
     1561 ggcgtcggcg attgggaaga gtaaagaatt acgaattacg aattaaaaat tacgaattac
     1621 gaattgggaa acattctttc attcgtaatt cgtaattttt aattcgtaat tcaaaaaata
     1681 ttgacttaaa aaccgaaaaa atataatatt tcccgcaagt tttctttctg aatattcgac
     1741 gcgggaagtt ttaagccgtt cgggacaatg aaaaccgctc gctttatcac ggtaaactct
     1801 ttctcgactc tcgttcccgt tcgccagaca tccgattccg ccgaatagcc gccgcgaccg
     1861 cgtgcaaggc aattaatacc ggttgtaaga cgaccgatac cgtgcgtcag acgactgata
     1921 ccgcgtgtaa aacgactgat accgcgtgta atatcacaaa taccgcgtgt aatatcacaa
     1981 ataccgcgtg taagacgatt gataccagac gaaagacagc cgcgcgcggg cgaaaattca
     2041 cttgacgctt gatttttgcg gctgaactca tacgaaagga gagataaaaa agatgactga
     2101 caaggaaatc agcgaattta attcggcggt cagaatgatc gactttaacg ccgcgaacgc
     2161 cgccgcgttt gccggtaatg cgaagatgac cggcggtttt gccgcgctcg cctccgaggt
     2221 cgaagtgctg gaatcggcgg gcgcaaaccg cgtttcggcg cgcggcatcc gcacggacgg
     2281 aacgctcgac aaaaacgccg ccaaaaccga ccttctcgct ttactgcgga aaatcgccga
     2341 gacggcgaag accatcaaaa aggatgaacc cgcgtttgaa aacacgttta aacttccgcg
     2401 cgggacgctc ggcagccagc aattgctcga cacggcgagc agctttgccg cgatgctgac
     2461 cgcgccgctc atcgccaggt tcggcgagtt cggactggct tcggcgacgc cggcaaatct
     2521 gaacgacaaa atcgccgcct ttcagaccgc ccgcgcccag caaaacgcgg gcaaaagcgt
     2581 cggcgtggct tcgaccgccg aaaccaaagc ctcgatcaaa cgcctgatgc aggcgcggcg
     2641 caccacagcc caaatcggcg aaaacatcct cgaagactcc ggcgacgccg gactgctcga
     2701 agcctggcgc tccgcctgcc gcatcgaaag gcacaaaacc aaacccgccg aaaacccgcc
     2761 gccacagccg ccgacgccgt gagggaactt aaacaggcgg cgaaaagatt gtcgagcgga
     2821 aaattttccg tgcaatacgc gcggaactta taactatgcg gaaatttttc ggcatagtta
     2881 taagttccgg ctctacgaga gtgattacgt ttaggaataa ttattatggc taactatcaa
     2941 gaaatcatca ccattgaccc acaagatgca gtcccacgaa ttcgccattt gaagatttca
     3001 gtgcaacaga ttcttgacct tatatcgaaa ggtataaccg aacaacaaat tttagaatta
     3061 caccgggcgt taactttaga ggacatcaat gcttgtcgag tttacgctgc cgtaaatcca
     3121 aagcgcgaaa attcattgga taagattatt ggagatttag aaaatacttc aaaacggtta
     3181 aattggggtg gttacatttt atttacaatt tcaataattc tgggaatcgt tctctatctt
     3241 gatgttagat ttaacacaat ttcacacatt gacaactact taaca
//
LOCUS       DASWNE010000979         3713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_628281, whole genome shotgun sequence.
ACCESSION   DASWNE010000979 DASWNE010000000
VERSION     DASWNE010000979.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628281"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..1612)
                     /locus_tag="VGB68_18990"
     CDS             complement(86..1612)
                     /locus_tag="VGB68_18990"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEY0051390.1"
                     /translation="MSSVEENLNVEEVISPAEARFEVWRNRAGFFLAPLVFLIIFFAP
                     LNLKPEAHRLAAVISAVVVLWICESLPMPVTALFGAALCVLLKVAPAKEVFAPFADPL
                     MFLFIGSFILARAIFLHDLDKRFAYGVLSLKLIGARPSRILFAFGAVTAFISAWISNT
                     ATTAMMFAIGLSIITFLFKQEKETGIKISRNYATALMLITSFAASIGGLATPIGTPPN
                     VIGLGFIRQIIGAEISFFKWMMIGAPIVVILYLFLYFYLNKLAPAGVKEITGSAEMIQ
                     RERASLGAWTRGQKSTVIAFSTAIALWVFSGLLALFVGETSEIYKAVNSRLPEAVGAI
                     AGAVLLFLLPGSAKGEKAITWNEAVKIDWGVILLYGGGFALGVLSFQTGLAEAIGRNL
                     TALLPLSGDFGLLVASVIVAVLISETTSNTASANMVVPVVIAIAQTQGADPLIPALGA
                     TFGASLGFMLPVSTPCNAIVYGSGYIPLMKMVRYGILLDVFGAIVIIIMLSLLAPFLR
                     "
     gene            complement(1805..3484)
                     /locus_tag="VGB68_18995"
     CDS             complement(1805..3484)
                     /locus_tag="VGB68_18995"
                     /inference="COORDINATES: protein motif:HMM:NF014598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HEY0051391.1"
                     /translation="MLTLLAVVFLIWLVFGYFAYGRWIAKQFRLDDARRTPANKINDN
                     EDFVPTKPFYLFGQHFSAIAAAGPIAGPIIACQAFGWLPCLLWIALGVVLIGAVHDFS
                     ALTSSIRHGATSIAQITREKLGNGAGRAMMAFIWIALVYVIVAFTDITAGTFVAGDEA
                     LQNASGFNPGGAVAFASVAYLGLSIVLGLVERFLKPPLWLSTIVFVPATFALSYVGTK
                     FSTVFLFDHITWGVLILIYCIIASLVPVWALLQPRGYLGGFVLYTAIAVGIIGIFFGG
                     YEIQQPHFKSFDVGGMTGMLFPFLFVTIACGACSGFHGLVCSGTTSKQLDKESHAKSV
                     GYGAMLAEGFVAFIALVTIMIVAQEASKGVAPGKIYGDGIGNFLTLLIGKENMAFAVT
                     FGAMAFSTFVFDTLDVSLRLGRYIVQELFGLKGRIGAIIGTLGTIFVPLICVLIAPKG
                     SWSDFWTLFGASNQLLAALTLLSITAWLYQARQRIAFTLLPMLFVLAITLWALTSMVV
                     GNFRAATGFDIKFVNGVASLALISLAIYLVVTAILKLRGDKRQSELAVENI"
BASE COUNT         1077 a          985 c          896 g          755 t
ORIGIN      
        1 attcaggatt caagatttag gattcaagat taaatccgat ttccaatcct gaatcctgaa
       61 tcctgaatcc tgaatcgttt atcgtttatc gaagaaacgg cgcgagcaaa ctgagcataa
      121 tgatgattac aatcgcgccg aaaacgtcga gcaggatgcc gtaacgcacc attttcatca
      181 atggaatata gcccgaaccg taaacaatcg cgttgcaggg cgtcgagacc ggaagcataa
      241 aaccgaggct tgcgccgaag gtcgcgccga gcgcgggaat cagcgggtcc gcgccctgcg
      301 tctgcgcaat cgcaatcact acggggacga ccatatttgc cgaagccgtg ttggaagtcg
      361 tctccgaaat cagtacggcg acgattaccg aggcgaccag cagaccgaaa tctccgctca
      421 gaggcaaaag agccgtcaga tttctgccga ttgcttcggc aagcccggtc tgaaaagaca
      481 aaacgccgag cgcaaatccg ccgccgtaaa gcaaaatcac gccccaatca atcttcaccg
      541 cttcgttcca cgtaatcgcc ttttcgcctt tcgcgctgcc gggcaaaaga aacaataaga
      601 ccgcgcccgc aatcgcgccg accgcttccg gcagacgcga atttaccgct ttgtaaatct
      661 cgctcgtttc gccgacaaaa agcgcgagca aaccggaaaa aacccacagc gcaatggcgg
      721 ttgaaaaagc gatgacggtc gatttttgcc cgcgcgtcca cgcgcccaga ctcgctcttt
      781 cgcgctgaat catctcggcg cttcccgtaa tttcttttac tccggcgggt gccagcttat
      841 tgagataaaa ataaagaaat aaataaagaa tgacgacaat cggcgcgcca atcatcatcc
      901 atttgaaaaa ggaaatttcc gcgccgatta tttgacggat aaagccgagc ccgataacgt
      961 tcggcggcgt tccgattggc gtggcaagcc cgccgattga agcggcaaac gacgttatca
     1021 gcatcagcgc cgtcgcgtaa ttgcggctga ttttgatgcc ggtttccttt tcctgcttaa
     1081 ataaaaacgt gatgattgac agcccgatgg caaacatcat agcggtcgtc gccgtgttgg
     1141 aaatccacgc cgagataaac gccgtgaccg cgccaaaagc gaataaaatc cgtgaaggtc
     1201 gcgcgccgat caatttcagc gaaagcactc cgtaagcaaa tcgcttgtcc aaatcgtgca
     1261 gaaaaatcgc ccgcgccaga ataaacgagc cgataaaaag aaacatcaac gggtcggcaa
     1321 acggcgcgaa aacctctttc gcgggcgcga ctttgagcaa aacgcagagc gccgcgccga
     1381 ataaagccgt caccggcatc ggcagagatt cgcaaatcca gagcacgacg acggcggaaa
     1441 tcaccgccgc caaacggtga gcttccggct ttaaattcaa aggcgcaaaa aatatgatta
     1501 gaaaaacgag cggcgcgaga aaaaatccgg cgcggttgcg ccagacctca aaccgggctt
     1561 cggctgggct gataacttct tcgacgttca gattttcttc gacagatgac atttattatt
     1621 aggaagagaa atttaaccac agatggacac agataaacat agattattca aacaggatag
     1681 agcaggcttc gcaacttgat ttacaggata aaatcttttt ctcatctatc tttgttttta
     1741 tctgcgttga tctgtgaaaa tccgtggttt cattttcttt tcgcgtattt cgcggacaat
     1801 gtatttaaat attctcgact gccagttcgc tctggcgttt atcgccgcgc agcttcagaa
     1861 tcgccgtcac gaccagataa atcgccagcg aaatcagagc cagcgatgcg acgccgttga
     1921 cgaattttat gtcgaagccg gttgccgcgc ggaaatttcc gacgaccata ctcgtcaaag
     1981 cccagagcgt gattgccagc acgaaaagca tcggcaaaag cgtaaaagcg attcgctggc
     2041 gcgcctgata aagccaagcc gtaatcgaaa gcagggtcaa agccgcgagc aattggttcg
     2101 acgcgccgaa cagcgtccag aaatcgctcc agcttccttt cggcgcaatc aaaacgcaga
     2161 taagaggcac gaaaatcgtt cccagcgttc cgataatcgc gccgattctg ccctttaagc
     2221 cgaaaagctc ctgcacgata tagcgcccca atcgcagtga aacgtccagc gtgtcgaaaa
     2281 cgaaagtcga aaaagccatc gcgccgaacg tgacggcaaa cgccatattt tcctttccga
     2341 ttaaaagcgt taaaaaattg ccgattccgt cgccgtaaat ttttccgggc gcgacgcctt
     2401 tcgacgcttc ctgcgcgaca atcataatcg tgacgagcgc gataaaagcg acgaagcctt
     2461 cagccagcat cgcgccgtag ccgaccgatt tggcgtgcga ttccttatcg agctgtttcg
     2521 atgtcgtgcc cgaacaaacc aatccgtgaa aaccggaaca cgcgccgcag gcaatcgtca
     2581 caaacagaaa cggaaagagc attccggtca ttccgccgac gtcgaacgat ttgaagtgcg
     2641 gctgctgaat ctcgtaaccg ccgaagaaaa tcccgataat gccgacggca atcgccgtgt
     2701 aaagcacaaa accgccgaga taaccgcgcg gctgtagcag agcccagacc ggaactaagg
     2761 atgcgataat gcagtaaatt aaaatcaaaa cgccccaagt tatatggtca aataaaaaaa
     2821 cggtcgaaaa ttttgtgccg acgtatgaaa gcgcgaaagt ggcgggaacg aaaacaattg
     2881 tcgaaagcca gagcggcggc tttaaaaatc tttcgaccaa gcctaaaacg attgacaaac
     2941 cgagataagc gacgctcgca aaagcgaccg cgccgcccgg attaaaaccc gaagcgtttt
     3001 gcagagcttc gtcgcccgcg acaaaagttc cggcggtaat gtccgtaaaa gcgacgataa
     3061 cgtaaaccag cgcaatccag ataaacgcca tcatcgcgcg ccccgcgccg ttgcccagtt
     3121 tttcgcgcgt gatttgggcg atcgacgtcg cgccgtgtcg aatcgaggaa gtcagagcgg
     3181 aaaaatcgtg taccgcgccg attaagacca cgccgagcgc aatccataaa agacacggca
     3241 gccagccgaa cgcctgacac gcgataatcg gaccggcaat cggacccgcc gccgcgatag
     3301 ccgaaaaatg ctgtccgaaa agataaaacg gcttggtcgg cacaaaatcc tcgttgtcgt
     3361 tgattttatt tgccggcgtc cggcgcgcgt cgtcgagccg gaattgtttc gcaatccagc
     3421 gcccataagc gaaatacccg aatacgagcc agattaaaaa gacgactgcc agaagcgtca
     3481 gcataaaatt ttctccgaat taatatctgg aaagatttga agctgaacaa atttacgata
     3541 tttcacagga aaacggcaaa taaaaaaaga aaatttacag ggataacagg atagacaggg
     3601 ataatatgaa tttttttcat aatccctgtt catcctgtta tccctgtaaa ttttcttctg
     3661 tcttaatctg tgtttaataa aatgaggtta tcagtctgaa tcggcggaat tta
//
LOCUS       DASWNE010000980         2746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_384139, whole genome shotgun sequence.
ACCESSION   DASWNE010000980 DASWNE010000000
VERSION     DASWNE010000980.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384139"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(231..788)
                     /locus_tag="VGB68_19000"
     CDS             complement(231..788)
                     /locus_tag="VGB68_19000"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEY0051392.1"
                     /translation="MSLKRKILTGITSGFAVIAFSSFAMAQDNSTTPQPDNQKSERKS
                     WKRGDGEGTRGGFRRGGHRGGFGMRGLRQLNLTDAQKEQIRTIMESNRGNREVNQTQR
                     EELRQIIEAKRSGTITAEQQERLRAFKEQRRAEARQTHQQILAVLTPEQRTQLEQLKQ
                     ERKERRKEFRQNRRDKTTDETSQDN"
     gene            1041..>2746
                     /gene="dxs"
                     /locus_tag="VGB68_19005"
     CDS             1041..>2746
                     /gene="dxs"
                     /locus_tag="VGB68_19005"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEY0051393.1"
                     /translation="MRFLSEINSPADLRQLRVEDLQEVANEVRQFIIDTTARVGGHTG
                     ASLGAVELAVAMHYVFETPGDKLVWDVGHQAYAHKILTGRRDQLHTIKKYGGLSGFLR
                     REESEYDTFGAGHASTSLSAALGMAVARDTKGEDFHVCALIGDSSLAGGMAMEAINQA
                     GHLKSRLIVLLNDNEMSIAPAVGALTRYLNRIKEAQSYQHLKEEIGDTLESVPHFGER
                     LRKAAKTVKDAIAAAVLPGALVNELGFKYIGYVDGHNVPMLVRALEEAKKVEDGPVIV
                     HALTTKGKGFPNPEKNYYAYHATGPYDAKTGKLIKSSKPAQPTYTEIFGHTMCELMDR
                     DESIVALTAAMPDGTGVDKILEKHPERSFDVGIAEQHAVTFCAGMACEGLKPVAAIYS
                     TFLQRGFDQIIHDVCLQNLNVTFAMDRAGIVGADGPTHHGLLDIAYLRGYPNIVLMSP
                     KDEGEMRDMILTAIEHPAPAAIRYPRGNGFGVDISEAPKLLEIGKAEILRDGGEIAVL
                     AYGSMVYPSMEAARNLEKDGVETTVVNARYVKPLDSELILALAQSKRLIVTVEEAYLA
                     GG"
BASE COUNT          620 a          688 c          770 g          668 t
ORIGIN      
        1 gaataccggt ttgaaggcgt ctttttcaaa ggcaaaaccg gcggtttgga tggagaaaaa
       61 gttttaagag gaactttgca aaagtttgtc aaaggcagaa aagcggcgga aatcgacgcc
      121 gatttcgctt attacgaacc gcgctgctgg cattgaaatt ttttaaatcg taacgaaaaa
      181 aggcgtggga aatcctacgc cttttaattt tgagttgagt tcgaaaaagc ctagttgtcc
      241 tgcgaagttt cgtcggtcgt tttatctcga cggttctggc gaaattcttt gcgacgctct
      301 tttctttcct gtttcaactg ttcgagctgc gttctctgtt ccggcgtcag gacagccaga
      361 atctgctggt gcgtttgacg cgcttcggcg cggcgctgtt ctttgaaggc tctcaggcgc
      421 tcttgctgtt cagccgtaat cgtgccgctg cgtttggctt cgatgatttg acgaagctct
      481 tcgcgctgcg tttgattgac ttcgcgatta ccgcgattgc tttccataat cgtgcgaatc
      541 tgttctttct gcgcgtcggt cagattgagt tgtctgagcc cgcgcattcc gaaaccgccg
      601 cgatgtccgc cgcgccgaaa accgccgcgt gtgccttcgc cgtcgccgcg tttccacgat
      661 ttacgttccg atttttgatt gtccggttgg ggagttgtgc tgttgtcctg cgccattgca
      721 aacgagctga acgcaatgac ggcaaaaccc gaagtaatac cggtaagaat ttttctcttt
      781 aaagacattg tgtttggtta cctctctaga attttttggt tggcttttgc tttccgctac
      841 ttttgatgct ttgcataagc gcttggtcga aatattttaa taaaccgcgt gtaaagaaat
      901 gtaaattacg gattcgactc gaatttataa attttattgg cgaactattt tcatcaatct
      961 tatcgtttat gatttaacat ttaggcggcg gcaagttaaa atgcgaaact tgagtatcta
     1021 tttcgagcgg aaaactttaa atgagatttt tatctgaaat taattcaccg gcggacttgc
     1081 ggcagctgcg cgtcgaagat ttgcaggaag tcgccaacga agtgcggcag tttattatcg
     1141 acaccacggc gcgcgtcggc gggcatacgg gcgccagcct cggcgcggtc gagctggcgg
     1201 tggcgatgca ttatgttttc gagacgcccg gcgacaaact ggtctgggac gtcggtcatc
     1261 aggcatacgc gcacaaaatt ttgacgggca ggcgcgacca gcttcacacc atcaaaaaat
     1321 acggcgggct ttccggtttt ctgcgccgcg aggaatccga atacgacact ttcggcgcgg
     1381 gacacgcttc gacttcgctt tcagccgcgc tcggaatggc ggtggcgcgc gacacgaaag
     1441 gcgaagattt tcacgtctgc gcgctgatcg gcgattcgag cctggcgggc ggaatggcga
     1501 tggaagccat caatcaagcc ggtcatttga aatcgcgcct gattgtttta ttaaacgaca
     1561 acgaaatgag catcgcgccc gccgtcggcg ctttgacgcg ctatctgaac cgcatcaagg
     1621 aagcgcagag ctatcagcat ttgaaggaag aaatcggcga cacgctggaa agcgtcccgc
     1681 atttcggcga gcgtttgcgg aaagccgcga aaaccgtcaa ggacgcgatt gcggcggcgg
     1741 tactgcccgg agcgctggtc aacgagctgg gttttaaata catcggttac gttgacggtc
     1801 acaacgttcc gatgctggtg cgcgcgctgg aagaagcgaa aaaggtcgag gacggtccgg
     1861 ttatcgtcca cgcgctgacg acgaaaggca aaggctttcc gaatcccgaa aaaaattatt
     1921 acgcttatca cgcgaccgga ccttacgacg ccaaaaccgg caaattgata aaatcgagca
     1981 agcccgcgca gccgacttat acggagattt tcgggcacac gatgtgcgaa ttgatggacc
     2041 gggacgaatc aatcgtcgcg ctgacggcgg cgatgccgga cggcacgggc gtcgataaaa
     2101 ttctggaaaa acatcccgag cgctctttcg acgtcggcat cgccgaacag cacgccgtca
     2161 cgttttgcgc gggaatggct tgcgagggct taaagccggt cgcggcgatt tattccacat
     2221 ttctgcagcg cgggttcgac cagattattc acgacgtctg cctgcagaat ctgaacgtca
     2281 cgtttgcaat ggacagagcc ggaatcgtcg gcgcggacgg tccgacgcat cacggcttgc
     2341 tcgatattgc ctacctgcgc ggctatccga acatcgtttt aatgtcgccg aaagacgaag
     2401 gcgagatgcg cgatatgatt ttgacggcga tcgagcatcc ggcgcccgcc gcgattcggt
     2461 acccgcgcgg aaacggtttc ggcgtcgata tttccgaagc gccgaagttg ctggaaatcg
     2521 gcaaagccga aattttgcgc gacggcggcg agattgccgt cctggcttac ggctcgatgg
     2581 tttatccttc tatggaagcg gcgcgaaatc tggaaaaaga cggcgtcgaa acaaccgtcg
     2641 tcaacgcgcg ctacgtcaaa ccgctcgatt cggaattgat tctcgcgctg gcgcagtcaa
     2701 aacgccttat cgtaacggtc gaagaagctt atctggcggg cggctt
//
LOCUS       DASWNE010000981         1544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_32176, whole genome shotgun sequence.
ACCESSION   DASWNE010000981 DASWNE010000000
VERSION     DASWNE010000981.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32176"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..613
                     /locus_tag="VGB68_19010"
     CDS             95..613
                     /locus_tag="VGB68_19010"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY0051394.1"
                     /translation="MSIVKYDPFRDLRSLQDEVNRLFSMNFPRSGGQDEIMRGAWTPS
                     VDIYEGKDEIVLEAELPGMKPEDVDVSIENNIITVKGERRFEKKDESDNYHRVERSYG
                     SFTRSFTLPRNVVGDRAKADFSNGVLHITLPKREEEKARKIQISGASSNESRTIETSA
                     TERQEKANTANA"
     gene            695..>1544
                     /locus_tag="VGB68_19015"
     CDS             695..>1544
                     /locus_tag="VGB68_19015"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEY0051395.1"
                     /translation="MRFDRYTIRGQEAIQEAIGVAEKNQNQQVEPEHLLAAMLEQKEG
                     TLRPILGKIGARTENILADVTAAIAKLPKVSGGQQYFSSRINTIFQTAQKEAEKMTDE
                     YISTEHLLLAIADEKEGDAGRILRSNAVTKEDIEKVVTDMRGGSRITDQNAEENFQAL
                     EKYALDLTERARKGKLDPVIGRDDEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIVEGL
                     AQRIVDKNVPSFLENKRILSLDLSLVVAGTKYRGQFEERLKKIMSELRENKEYVVFID
                     ELHTLVG"
BASE COUNT          459 a          356 c          411 g          318 t
ORIGIN      
        1 ttattattat tacagactaa gcaaacaaaa ctttccgttt gagtttgttt ggtcaaatga
       61 tgcacaaaga aatttttttt ggaggaaaat tacaatgagc attgttaaat atgacccttt
      121 tcgtgattta cgcagtttgc aggatgaagt gaatcgcctg ttttcgatga attttccgcg
      181 cagcggcgga caggatgaga ttatgcgcgg cgcctggacg ccgagcgtag acatctacga
      241 aggcaaagac gaaatcgtgc ttgaagccga actgccggga atgaaaccgg aagacgtcga
      301 tgtttcgatt gaaaacaata tcattaccgt taaaggcgag cgccggttcg agaaaaaaga
      361 cgaatcggat aattatcatc gcgtcgaacg cagctacggc tcgtttacgc gctcgtttac
      421 gctgccgcgc aacgtcgtcg gcgaccgggc gaaagccgat ttttcaaacg gcgttctgca
      481 tataacgctg ccgaagcggg aagaagaaaa agcccgaaaa attcaaattt cgggcgcatc
      541 gtcaaacgag tcgcgcacga ttgagacgag cgcgaccgag aggcaggaaa aagcaaacac
      601 ggctaatgcc tgatttttta aacgataaac gcggaacgat gaacgatgaa cgacaaattc
      661 cgcgttcatc gttcatcgtt catcatttta agttatgaga ttcgacagat atacaattcg
      721 cggacaggaa gcgattcagg aagccatcgg agtcgccgaa aaaaaccaga accagcaggt
      781 cgagccggaa catctgctcg cggcgatgct cgagcaaaag gaaggtacgc tgcgccctat
      841 cttaggaaaa atcggggcgc gcaccgagaa tattttagcg gacgtaacgg cggcgatcgc
      901 gaaacttccg aaagtgtccg gcggacagca gtatttttct tcgcgcatta acacgatttt
      961 tcaaaccgcg cagaaggaag ccgaaaagat gacggacgaa tatatctcga ccgagcatct
     1021 gctgctggcg attgccgacg agaaagaagg cgacgccgga cgaatcctgc gctcaaacgc
     1081 cgtgacgaag gaagacatcg agaaagtcgt caccgatatg cgcggcggct cgcgcatcac
     1141 cgaccagaac gccgaagaaa actttcaggc gctggaaaaa tacgctctgg atttaacgga
     1201 acgagcgcgc aaaggaaagc tcgacccggt catcgggcgc gacgacgaaa tcagaagaac
     1261 gattcaggtt ctttcgcgcc ggacgaaaaa caatccggtt ttaatcggcg agccgggcgt
     1321 cggcaaaacg gcgattgtcg aaggcttggc gcaaagaatt gtcgacaaaa acgtgccgtc
     1381 atttcttgaa aacaaacgca ttttatcgct cgatttgtca ctggttgtcg ccggcacaaa
     1441 atatcgcggg caatttgaag aacggcttaa aaaaatcatg agcgaacttc gtgaaaacaa
     1501 ggaatatgtt gttttcattg atgagctgca cacgctcgtc ggcg
//
LOCUS       DASWNE010000982         1602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_32219, whole genome shotgun sequence.
ACCESSION   DASWNE010000982 DASWNE010000000
VERSION     DASWNE010000982.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32219"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="VGB68_19020"
     CDS             complement(<1..968)
                     /locus_tag="VGB68_19020"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEY0051396.1"
                     /translation="MLIEKIRSLTIEQKIGQLFFIGLPGAEVDADTRRLLEEISPGGI
                     CLFSRNVRSVEQVRRLTEDLREILPVEPIISIDQEGGLVDRLRRIVTPMPPPRVIREH
                     GDLAAARRLGAITGDILRMLGLNMNFAPTMELLSEDRELLSNGLYSRSFGRSPGEVLG
                     YSMVYLRGLQSAGVLGCLKHFPGIGAGEVDSHEELPLVHLSHDELVAKDLAPYIELFF
                     FRRDEDDRVRAVMVGHGGYPKIDLESETIGGPLVPASINANIVTKLLRDELGYKHLVL
                     TDDMEMGAILKHCPIEEATKAAFKAGEDMILICASAESMRKSYQAM"
     gene            1238..>1602
                     /locus_tag="VGB68_19025"
     CDS             1238..>1602
                     /locus_tag="VGB68_19025"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEY0051397.1"
                     /translation="MNRKILIFAFGWLAIISLYQTAFAQKICDGKQLFSEKTCAGDTL
                     DAREKELFRLINEYRAQNKLPPLVLSDALSVVANRHLLDLNINVKYLTHGWSNCPYDI
                     KNQNTWNCVLKAPKMLGVK"
BASE COUNT          418 a          417 c          387 g          380 t
ORIGIN      
        1 atcattgcct gatagctttt tctcatagat tcggcgctgg cgcaaatcaa aatcatatcc
       61 tcgcccgcct taaaagccgc tttggtcgct tcttcaatcg ggcaatgctt taaaatcgcg
      121 cccatttcca tatcgtcggt caaaaccaga tgcttgtagc cgagctcgtc gcgcagcaat
      181 ttggtgacga tgtttgcgtt aatcgaagcc ggaaccagag gaccgccgat ggtttcgctt
      241 tccagatcga ttttcggata accgccgtga ccgaccatca cggcgcgcac gcggtcgtct
      301 tcgtcgcggc gaaagaaaaa aagctcgata taaggcgcga ggtctttggc gaccagctcg
      361 tcgtgcgaca gatgcaccag cggaagctct tcgtgcgaat cgacttcgcc cgcgccgatg
      421 cccggaaaat gtttcagaca gccgagcacg cccgcgctct gaagcccgcg caggtaaacc
      481 atcgaataac ccaaaacttc gccgggcgag cgcccgaacg agcgcgaata taaaccgttg
      541 gaaagcagct cgcggtcttc ggacaacaat tccatcgtcg gcgcaaaatt catatttaaa
      601 ccgagcatcc gcaggatatc gccggttatc gcgccgagtc gtcgggcggc ggctaaatcg
      661 ccgtgctcgc ggatgacgcg cggcggcggc atcggcgtga cgatgcggcg caggcggtca
      721 accaaaccgc cttcctggtc gatactgatt atcggctcga ccggcagaat ttcccgcagg
      781 tcttcggtta accggcgcac ctgttcgacg ctgcgcacat tgcgtgaaaa aagacagatt
      841 ccgccgggcg agatttcttc gagcagacgg cgcgtgtcgg catcgacttc cgctcccgga
      901 agcccgataa aaaataattg tccgattttt tgttcgatgg taagagaccg aattttttct
      961 atcagcatga taacttcagc ggattttagc aaggttaaag tgatttataa agcgtcggca
     1021 cgctgacagt taaagtaaag cttaaaaata ttcgcaaaag ccttttattt acagcgtttc
     1081 aggagtcaaa tgtttctttt tttcaagttc ggtttaatca atttcagcat taacccgcaa
     1141 agcttcttga cattttataa aactcggcat actttgcttt aaacaaaagc cccgtacttg
     1201 ttcagatata aatttacaaa acaaaaataa taaagttatg aatcgcaaaa ttttgatttt
     1261 tgcctttgga tggctggcga ttatttcgct ttatcaaacg gcattcgcgc agaaaatctg
     1321 tgacggaaaa caattgttca gcgaaaaaac gtgtgccggc gatacgctcg acgcgcggga
     1381 aaaagagctt ttccggctta tcaacgaata ccgcgcgcag aacaaactgc cgccgctcgt
     1441 tttgtcggac gcgctgagcg tcgtcgccaa ccgccacctg ctggacttga atatcaacgt
     1501 caaatatctg acgcacggct ggagcaattg cccgtatgac atcaaaaatc aaaacacctg
     1561 gaattgcgtt ttaaaagctc cgaaaatgct cggcgtaaaa ta
//
LOCUS       DASWNE010000983         4852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_32230, whole genome shotgun sequence.
ACCESSION   DASWNE010000983 DASWNE010000000
VERSION     DASWNE010000983.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32230"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1368
                     /locus_tag="VGB68_19030"
     CDS             <1..1368
                     /locus_tag="VGB68_19030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051398.1"
                     /translation="RYDFRQRFHSAKEAFAALNTIASSLGYGRKQQNFVQPKIFQPVQ
                     PVAAQAPNVFQNPVAALVPQAPAPQEPLPPPAPTQPAPQQNLPPTIIYSPQPHFQPEY
                     KKQAEYQRVEYPKQESKGFLQWIWNNDLALGVFVAALVLGFFFIGGYVLISSAIKTAD
                     AEARKNAPASNSAVTGATPFPSPADSGAYQEALSQAAEASKIEKKATTKFEWEEIGNK
                     YKRASTLLASVHESSPDYVDAQAKIADYKQRADSAFQKAGDARSNPQLLTNTNSQYSN
                     SQYSTNPTTVSTNYPSTTSPATAPRPLKIARQPAPRREKSFLVFNSSEGDYIGKGLDW
                     VFTSDESKFTANGGSKRYLNLGVDGNDKWFHLTLSVPDNLVFQPGSYPSAGYRQGGNS
                     NAYPSLSFSGDGRGCTAGDASFTIHNIQYGEMNSELLYIDVSFRQRCQNTSAVLLGRF
                     RYDVR"
     gene            1685..>4852
                     /locus_tag="VGB68_19035"
     CDS             1685..>4852
                     /locus_tag="VGB68_19035"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY0051399.1"
                     /translation="MKAENSFLHAVHRFAALVLIYVFVFLGIGVVPQRAQEKNANLFD
                     KFAGADAVTSAVRSKSKILQRVALRSEAERRALEKLGTIVEDYGSFVILATSETTDLS
                     RFDAAKVETTINLPARKYEPVKTPPAETVRPNRPENINANEKDYYIVQFAAATKDVWL
                     ESLREIGAEIVQYVPHQAFFVYADGAMIARIANHSRVRWVGRYTAGDKLSPELRHFTD
                     GVRGNTAMFDIAVFSRANLEEVARRISSSFSGRILSTIKLPNNYFNVLRVEMPINELA
                     QIAVISDVVRIDQYIRPEREDERAAQIVAGNYTSPTSILAPGYNPLTQFGVDGTGVTV
                     SVVDDGVSIPGNGGFYINSANTVDGPLRGATAGAAGGHGHINASIIAGSTPFGSLDPT
                     GYNYGIGIAPRTNIINIPFLKSPYAGSDAQVIDDSLNTPGPNGVRGTISNNSWGDGTN
                     GNSYDSLAAQYDGFVRDGSLASTIDPFNIIFSAGNSGPGALSLTRPKVAKNVISVANS
                     ENIRTEIGGTGADNMDDLRSSSSRGPAADGRVKPDITAPGTVIAGSRGGSGSTVSGII
                     DANHAWSSGTSHAAPQVAGAAALFTQYWKNTHVGQYPNPSLVKAAILNTAQEMNGLTT
                     NTSTIPNGNEGWGRINMKYMLNTGVPMKYINEEIALENAGTGIAVIGTVADSTKPVRI
                     TLVWTDPPGVSNPALVNNLDLQVNVNGTFYRGNVFAGGVSTTGGAGDTLNNVENVFLP
                     AGIPAGSFFSVSVGAAALNGDGILGNADTTDQHFSVVAYNFRELSVPTINKIADFDGD
                     GKTDVSVFRPSSGDWYVTNSGNNQFVATHFGQNGDIPAPGDYDGDGRTDRAVFRPSDG
                     TWYILRSGGGFSATQFGQNGDVPTAGDYNGDGITDVSVFRPSNGSWYRINSSGGQFVA
                     VQFGANGDKAVPADYNGDGATDLAVWRPSDGVWYQLRSGQGFAATQFGANGDKPAQGD
                     FDGDGKTDLAVFRPADGTWYLLRSQAGFTATQFGIGSDTPAPGDYDGDGKTDLAVYRD
                     GIWYQLRSTSGFTAVQFGIAGDKPIP"
BASE COUNT         1160 a         1377 c         1322 g          993 t
ORIGIN      
        1 cggtacgatt ttcgccagcg ctttcattcg gcgaaagaag cttttgccgc gctgaatacg
       61 attgcttcga gcctcggtta cggcagaaaa caacaaaact ttgtccagcc gaaaattttt
      121 cagcccgtcc agccggtcgc cgctcaagct ccgaacgtct ttcaaaatcc ggtcgccgct
      181 ctggttccgc aagcgcccgc gccgcaggaa cctttgccgc cgccagcgcc gacccagccc
      241 gcgccgcagc agaatctgcc gccgaccatc atttattcgc cgcagccgca ttttcagccc
      301 gaatataaaa agcaagccga atatcaaaga gtcgaatatc cgaaacagga aagcaaagga
      361 tttctccagt ggatttggaa caacgacctc gcgctcggcg tgtttgtcgc cgcgctggtg
      421 ctcggtttct ttttcatagg cggttacgtt ttgatttctt ccgccatcaa gaccgccgac
      481 gccgaagcgc gcaaaaacgc gccggcgagc aattccgccg tcacgggcgc gacgccgttt
      541 ccgagtccgg cggattccgg cgcatatcag gaagctttat cccaagctgc cgaagcctcg
      601 aaaatagaga aaaaagcgac gaccaaattc gaatgggaag aaatcggcaa caagtataag
      661 cgcgcctcga cgctgctcgc ctcggttcac gagtcaagcc cggattacgt cgatgcgcag
      721 gcgaaaatcg cggattacaa acagcgcgcg gacagcgctt ttcaaaaagc cggagacgcc
      781 cggagcaatc cgcaattatt aacgaataca aactctcaat attcaaactc tcaatattcg
      841 acgaatccga cgaccgtctc gaccaattac ccttcgacga cgagcccggc gacagcgccg
      901 cgcccgttga aaattgcgcg ccagcccgcg ccgcgccgcg aaaagtcgtt tctcgttttc
      961 aacagttccg aaggcgatta tatcggcaag ggcttggatt gggttttcac ttccgacgaa
     1021 agcaaattta cggcgaacgg cggctcgaaa agatatttaa atctcggcgt cgacggcaac
     1081 gacaaatggt ttcacctgac gctcagcgta ccggacaatc tggtttttca acccggaagt
     1141 tacccgagcg ccggttacag gcagggcggc aacagcaacg cgtatccgtc gctcagtttc
     1201 agcggcgacg ggcgcggctg cacggcgggc gacgccagct ttacgattca taacattcaa
     1261 tacggcgaga tgaacagcga gcttttgtat atcgacgtca gctttcgcca gcgctgccag
     1321 aacacttcgg cggttttgct cggcagattc cgctacgacg ttcgttagac attttttagc
     1381 cgccgtgaaa aaagcaaaag taaaaaggta aagggcgaaa ttaagattta gcttttcgcc
     1441 ataaaatccc gtcggctgag ttttcgtttc aaacctgatt gaacgcgaga atcgctcgac
     1501 gaattcgggc gataaacccg cgattttcaa agcctgattc tgcggcgcga ttgattaaat
     1561 actctaaatt aacgcttctg cttcgtaaat aaaaaacact gtgctaattt attagctacc
     1621 acaaaggcaa accgtcagaa gtttaccagt cccggctttt taattacatc aggagtcagc
     1681 aggaatgaaa gcagaaaata gctttttgca cgccgtacac cgtttcgccg cgctcgtctt
     1741 gatttacgtt ttcgtttttc tcggaatcgg agtcgtcccg cagcgagcgc aggaaaaaaa
     1801 cgccaatctt ttcgataaat tcgccggagc cgacgcggtg acgagcgccg ttcgcagcaa
     1861 atcgaaaatt ttgcagcgcg ttgcgttgcg cagcgaagcg gagcgccgcg ctctggaaaa
     1921 gctcggcacg attgtcgaag attacggctc tttcgtgatt ttagcgacga gcgaaacgac
     1981 cgatttatcg aggtttgacg ctgcgaaagt cgaaacgaca atcaatctgc ccgctcgaaa
     2041 atacgagccc gtcaaaacgc cgcccgccga aaccgtcaga ccgaaccgac cggaaaatat
     2101 caacgcgaac gaaaaagatt attacatcgt gcagtttgcc gccgcgacaa aagacgtctg
     2161 gctcgaaagc ctgcgcgaaa tcggcgcgga aatcgtgcaa tacgttccgc accaggcgtt
     2221 ttttgtttac gccgacggcg cgatgattgc ccgaatcgcc aatcattcgc gcgtccgctg
     2281 ggtcgggcgc tacacggcgg gcgataagct ttcgcccgag ctgagacact ttacagacgg
     2341 cgtgcgcggc aacactgcaa tgttcgacat cgcggttttt tcgcgcgcca atctggaaga
     2401 agtcgcgcgc cggatttcca gctcgttttc gggaagaatt ctgagcacta tcaagctgcc
     2461 gaataattac tttaatgttc tgcgcgtcga aatgccgatt aatgagctcg cgcaaatcgc
     2521 cgtaatttcc gacgtcgtgc ggattgacca atacatcaga ccggaacgcg aagacgaacg
     2581 cgccgcgcaa atcgtcgccg gaaactacac cagcccgacc agcattttag cgccgggcta
     2641 taaccctctg acccagtttg gcgtggacgg cacgggcgta accgtttcgg tggttgacga
     2701 cggcgtaagc attcccggca acggcggttt ttacattaat tcggctaata cagtcgacgg
     2761 accgcttcgc ggcgcaaccg ccggagccgc cggcggtcac ggacacatca acgccagtat
     2821 catcgccggg agcacgccgt tcggctcgct cgacccgacc ggttacaact acgggatagg
     2881 catcgcgcca agaaccaata ttattaatat tccgttttta aagagcccgt acgcgggcag
     2941 cgacgcgcag gtgattgacg atagtttaaa cactccgggt ccgaacggag tccggggcac
     3001 aatctctaat aacagctggg gagacggcac gaacgggaat tcttacgatt cactcgccgc
     3061 gcagtatgac ggttttgttc gcgacggctc gctggcgtcg acgattgacc cgtttaatat
     3121 tatcttttcg gcgggaaatt ccggtccggg cgcgctcagc ctgactcgtc cgaaggtggc
     3181 gaaaaacgtt atctccgtcg ccaattccga aaacatcaga acggaaatcg gcggaaccgg
     3241 cgcggataat atggatgatt tacgcagcag ttccagtcgt ggtccagccg cagacgggcg
     3301 cgtcaagccc gatattaccg cgccgggaac ggttatcgcg ggctcgcgcg gcggaagcgg
     3361 ctctaccgtc agcggaatta tcgacgccaa tcacgcctgg agcagcggaa cgtcgcacgc
     3421 cgcgccgcag gtcgccggag cagcggcgct tttcacccaa tactggaaaa atacgcacgt
     3481 cgggcaatat ccgaatccat cgctggtcaa agccgcgatt ctcaacaccg cgcaggaaat
     3541 gaacggtttg acgaccaaca cctcgacgat tccgaacggc aacgaaggct ggggacgcat
     3601 caatatgaaa tatatgctca atacgggcgt cccgatgaaa tatatcaatg aggaaatcgc
     3661 cctggaaaat gccggaaccg gcatcgccgt tatcggaacg gtggcggatt cgaccaagcc
     3721 ggtgcgcatt actctggtct ggacagaccc gccgggagtt tccaatccgg cgctggtcaa
     3781 caacctcgat ttacaggtca acgtcaacgg aactttttat agaggaaatg tttttgcggg
     3841 cggcgtctcg acgacgggcg gagccggcga cacgctcaac aacgtcgaaa acgtttttct
     3901 tccggcaggc attccggcgg gaagcttctt tagcgtcagc gtcggcgcag cggcgctgaa
     3961 cggcgacgga attttaggca atgccgacac gaccgaccag cacttttcgg tcgtagctta
     4021 taatttccgg gaattaagcg tcccgacaat caataaaatt gccgattttg acggcgacgg
     4081 caaaacggac gtttccgttt ttcgaccgtc gagcggcgac tggtatgtta ctaatagcgg
     4141 caataatcaa tttgttgcca cacacttcgg acaaaacggc gacattcccg cgcccggcga
     4201 ttatgacggc gacggcagaa ccgaccgcgc tgtcttccga ccgtcggatg gaacctggta
     4261 tatcttgcgc agcggcggcg gtttttccgc gacgcagttc ggacaaaacg gcgacgttcc
     4321 gaccgccggc gattacaatg gcgacggcat cacggacgtt tccgttttcc gtccttcaaa
     4381 cggcagctgg tatcgaataa acagttcggg cggtcaattt gttgccgtgc aattcggcgc
     4441 gaacggcgat aaagcggtgc ctgccgatta caacggcgac ggggcgaccg acctcgccgt
     4501 ctggcgacct tccgacggcg tttggtatca attacgctca gggcagggct ttgccgcgac
     4561 gcagttcggc gcaaacggcg ataaaccggc gcagggcgat ttcgacggcg acggaaagac
     4621 cgatttggcg gttttccgcc cggctgacgg aacgtggtat ctgcttcgct cgcaggcggg
     4681 ctttaccgcg acgcagttcg gcatcggcag cgatacgccc gcgcccggcg attacgacgg
     4741 cgacggcaag acggatttgg cggtttatcg cgacggcatc tggtatcaac tgcgctcgac
     4801 ttccggattt acggcggttc aattcggaat tgcgggcgat aaacccatac cg
//
LOCUS       DASWNE010000984         2240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_564279, whole genome shotgun sequence.
ACCESSION   DASWNE010000984 DASWNE010000000
VERSION     DASWNE010000984.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564279"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..1416)
                     /locus_tag="VGB68_19040"
     CDS             complement(25..1416)
                     /locus_tag="VGB68_19040"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEY0051400.1"
                     /translation="MEKVERLKAELDSRRPLDAETEARIMQKFRLDWNYHSNNLEGNS
                     LTYGETKALILFGITAQGKPLRDHFEITGHNEAINWILEIVKDERPLSETFIRELHTL
                     LLKESSYKEALTPEGAPTRRKIEVGKYKTQPNHVVTVTGETFYFAKPEETPAKMQELV
                     EWFRDEKEKSGVNPIILSALFHYRFIRIHPFDDGNGRVARILMNFILMQFGYPPAIIK
                     TEDKENYYSVLRLADADELEPFIEYIAQNLTRSIEIMIKGAQGEDIEEPDDFDKELAF
                     LEKKFESAGKKIEKAKTKDTVLEVIDCVLPNFIKEFLVANSKFNRFYLDSSFDLQLIQ
                     GSNISSKLMFSTLYTEKIRSISNKDIEKIIEKINPIILKLFESNNFEKFVGIGLKYSF
                     SALNIFEVNAEFGAFVGIEFHEKYYLISNINLQESKQFLYTEKPGNTEIIKVIKAESI
                     RHKNHLDNILTKS"
     gene            complement(1465..>2240)
                     /locus_tag="VGB68_19045"
     CDS             complement(1465..>2240)
                     /locus_tag="VGB68_19045"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEY0051401.1"
                     /translation="GVFGVAIGTAAIPALSRLAAEENFGKFRTTLSDAIRLVFFLTLP
                     SACGLIVLGEPIIRLIYERGLFTAASTQMTAFALAGYSIGLTGYAAIKVLSPSFYALD
                     DVKTPMTVSLVSVVIYPFAGYFLMQYFSQFHVTPETPKGLAHVGLTVATSVIALTSFS
                     LLTFMMRRRIKRIDGKEVLTALIKIAVASAVLSAVAYAAYYGLATLLGTRGLIARLVE
                     VFVPIALGGIAFLVTAKLLNIEEMNRAYDAFARKLKSKK"
BASE COUNT          598 a          492 c          494 g          656 t
ORIGIN      
        1 ttgattaaat tattgaacaa acagttagct ttttgtaaga atgttgtcta aatgattttt
       61 atgtctaata gattcagctt taataacttt aataatttct gtattgcctg gtttctctgt
      121 gtataagaat tgtttgcttt cttgaagatt aatattactt attaaatagt atttttcgtg
      181 gaattctata ccgacaaatg ctccaaattc agcatttacc tcaaatatat ttaaagcgga
      241 gaaagaatat tttaaaccta ttcccacaaa tttttcaaaa ttattacttt caaacaattt
      301 taatattatt gggtttattt tttctataat tttctcaata tccttgttac taattgatct
      361 aatcttttct gtgtaaagtg tcgaaaacat caatttacta cttatgttac tgccttgtat
      421 taactgtaaa tcaaaactgg aatctaaata aaatctatta aattttgaat ttgcaactaa
      481 aaactcctta ataaaatttg gaagaacgca atcaataact tctaaaacgg tatctttagt
      541 cttagccttt tcaatctttt tacccgctga ctcaaatttc ttctctaaaa atgccagttc
      601 tttatcaaaa tcgtcaggtt cttcaatatc ttcgccttgc gcgcccttaa tcattatttc
      661 aattgaacga gtaagatttt gcgcgatgta ttcgataaat ggctcaagct cgtcagcgtc
      721 ggcaaggcgc aggacggagt agtaattttc tttgtcttcg gttttgataa tcgcgggcgg
      781 atagccgaac tgcatcagga taaaattcat taagattcgc gcgactctgc cgttgccgtc
      841 gtcgaatgga tgaatgcgga taaatctgta atgaaaaagc gcggatagga taatcggatt
      901 tacgccggac ttttcctttt cgtctctaaa ccattcgact aactcctgca ttttggcggg
      961 cgtttcttcc ggcttggcga aataaaaagt ttcgcccgtg acggtgacga cgtgattggg
     1021 ctgagttttg tatttgccga cttcgatttt tcggcgcgtc ggcgcgcctt cgggagttaa
     1081 ggcttctttg tagctcgatt ctttcagtaa aagcgtatga agctcgcgga taaacgtttc
     1141 gctcaaaggt ctttcgtctt tgacaatctc taaaatccaa tttatcgcct cgttgtgccc
     1201 ggtgatttca aaatggtctc tgagcggctt gccctgcgcc gtgatgccga acaaaattag
     1261 agctttggtt tcgccgtagg tgagagaatt accttccaga ttgttcgaat gataattcca
     1321 gtcaagccgg aatttttgca taatccgcgc ttcggtttcc gcgtctagcg gacgccggct
     1381 gtcgagttcg gcttttaatc tttcaacttt ttccaaggct tccatagctc acattttcaa
     1441 acgtttaatt aaggtttgca acgattattt tttcgatttc agctttcgcg cgaaagcgtc
     1501 gtaggcgcgg ttcatttctt cgatgttcag gagctttgcc gtgacgagaa aagcgattcc
     1561 gccgagagcg atgggaacga aaacttcgac caggcgcgcg attaagccgc gcgtgccgag
     1621 cagcgtcgcc aagccgtaat aagcggcgta ggcgacagcc gaaagaacgg ctgaggcgac
     1681 ggcgattttt attaatgccg tcagaacttc tttgccgtcg atgcgcttga ttcgcctgcg
     1741 catcataaag gtcagaagcg aaaagctcgt cagcgcgata acggaagtcg ccaccgtcaa
     1801 gccgacgtgc gccaagcctt tcggcgtctc gggcgtgacg tgaaactgcg agaaatactg
     1861 cattaaaaaa taaccggcga aaggataaat gacgaccgag acgaggctga cggtcatcgg
     1921 cgttttcacg tcgtccagcg cgtaaaaaga cggcgaaaga acttttatcg cggcgtagcc
     1981 cgtcaagccg attgaataac cggctaaagc gaaagccgtc atctgggtcg aagcggcggt
     2041 aaacagtccg cgctcataaa tcaggcggat aatcggctcg cccagaacaa tcaagccgca
     2101 cgccgaaggc agcgttaaaa agaaaaccag acgaatcgcg tcggaaagcg tcgtgcggaa
     2161 ttttccgaaa ttttcttccg ccgccagacg cgaaagcgcc ggaatcgccg ccgtgccgat
     2221 ggcgacgccg aaaacgccta
//
LOCUS       DASWNE010000985         2670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_172027, whole genome shotgun sequence.
ACCESSION   DASWNE010000985 DASWNE010000000
VERSION     DASWNE010000985.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172027"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(330..2147)
                     /locus_tag="VGB68_19050"
     CDS             complement(330..2147)
                     /locus_tag="VGB68_19050"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEY0051402.1"
                     /translation="MFQKGFIFFLLLNLAFGVSAQNTMSGGSSTGDARTYSSRRTTGI
                     TEAGAPKVFEDVTTQTALKNFVQTGGDKAKNYLPESTSGAVAVLDFDNDGKLDIYLLN
                     GGTIDALQGKEKMPRAALFRNLGDWKFEDVTGKAGVANERWAMGAAVGDYNNDGFPDI
                     FVSNFGAARLYKNNGDGTFTDVAEKVGLNVSGWFTGASFGDYDRDGRLDLFVPAYIEF
                     DLAKLPVSPADIARNGNVGRNFCQFRGQPTMCGPRGLTGAKDRLFHQKADGTFEDASA
                     KTGITDTEKYYGFSSVFADIDDDRDLDLLVVNDSTPNQLYINKGDGTFEETGYPSGIA
                     LNENGREQAGMGLAVGDYDNDGRVDFHITNFSDDSNTLYRNDGDANFTDVTFQTGLGE
                     TTIPFLGWGTLFFDFDNDGWKDILVANGHIYPQVDNFQWGTSYAQQLLLFRNRQSDAK
                     KKPVGFERVAAAPNSGLAIAIPARGLAVGDFDGDGREDAVINNLDSAPTLLRNVTASK
                     NHWLSLRLVGDAAKKTPKDAIGSTVFVTAGNLRQRFDVFSGASYASQSDQRLHIGLGA
                     AGKIDRLEIRWAGGNVETINIPGVDRAIVIGEGKGLITK"
     gene            complement(2190..>2670)
                     /locus_tag="VGB68_19055"
     CDS             complement(2190..>2670)
                     /locus_tag="VGB68_19055"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEY0051403.1"
                     /translation="QLAANGAEILVRVSAYMDPWNATAPMDWWTVINRARAIENMAYV
                     VAANQAASLRHYPPYSWSGGSQIVDFEGRLLADASPGPGERIVVAPVDISALRHERGT
                     RRGHHMLAHLRTEAYPVYQNHLYPPQSENAEAGEHLSYEKNNELIDEAKRRVDARDF"
BASE COUNT          629 a          747 c          664 g          630 t
ORIGIN      
        1 attaacatca gcagcgcggc tgccgatagt cttagtggct tcatacttcc aactccttaa
       61 atttagtaac tgaatagctt aaatcaataa tgactgaaaa aaacagaata ttttagactt
      121 gcccgtcttt aaacgaattt ataaacacgc cggcttaaaa agatttaaaa taaccttcga
      181 aaattaatta aattttatta aaaaatattg ttggttaagc ctttacgtaa acgtttaact
      241 tatatgccag tattaatcac ctgcgctgta gttgtcaatc agttatgaaa ataattcatc
      301 gggtcgatgc agaatttcga tagagtaatt tatttcgtaa tcagcccctt gccttcgccg
      361 atgacaattg cgcggtcaac gccgggaata tttatcgtct caacatttcc gcccgcccac
      421 cgaatctcca gcctgtcgat ttttcccgct gcgccgagcc cgatatgaag acgctggtcg
      481 gactgcgatg cgtagctcgc gccgctgaaa acgtcgaaac gctgccggag atttccggcg
      541 gtgacgaaaa cggtcgagcc gatggcgtct ttcggcgttt tctttgccgc gtcgccgacg
      601 agcctgagcg aaagccagtg atttttcgaa gccgtcacgt ttcgcaaaag agtcggcgcg
      661 gaatccagat tattaatcac ggcgtcttcg cgcccgtcgc cgtcaaaatc tccgacggct
      721 aaccctcgcg ccggaatggc aatcgccaag ccgctgttcg gcgcggcggc gacgcgttca
      781 aacccgaccg gcttcttttt ggcgtcagac tgccggttgc gaaaaagcag caactgctgc
      841 gcgtagctcg ttccccattg aaaattatcg acctgcgggt aaatatgacc gttggcgacg
      901 agaatgtctt tccagccgtc gttgtcgaaa tcgaaaaaca aagttcccca gccgagaaac
      961 ggaatcgtcg tctcgcccaa gccggtctga aaagtcacgt ccgtaaaatt cgcgtcgccg
     1021 tcgttgcgat aaagcgtgtt cgaatcgtcg gaaaaattcg tgatatgaaa atcgacgcgc
     1081 ccgtcgttgt cgtaatcgcc gaccgccaaa cccattccgg cttgctcgcg cccgttttcg
     1141 ttcagcgcga tgcccgaagg ataaccggtt tcttcaaacg tgccgtcgcc tttatttata
     1201 tataattgat tcggcgtcga atcgttgacg accagcaaat ccaaatcccg gtcgtcgtcg
     1261 atatcggcga aaacggacga aaaaccgtaa tatttctccg tgtccgtgat gcctgttttg
     1321 gcggaagcgt cttcaaaggt gccgtccgct ttctggtgaa aaagacggtc tttcgcgccc
     1381 gtcaagccgc gcggaccgca catcgtcggt tgaccgcgaa actggcagaa atttcttccg
     1441 acgttcccgt tcctggcgat atccgcagga cttacgggca atttcgccaa atcgaattcg
     1501 atataagccg gaacgaacaa atcgagccga ccgtcgcggt cgtaatcgcc gaaactggcg
     1561 cccgtaaacc agcccgaaac gtttaagccg accttttcgg caacgtccgt aaacgtgccg
     1621 tcgccgttgt ttttataaag ccgcgccgcg ccgaaattgc tgacgaaaat atcgggaaag
     1681 ccgtcgttgt tgtaatcgcc gaccgccgcg cccatcgccc agcgctcgtt ggcgacgccc
     1741 gctttaccgg tcacgtcctc aaatttccaa tcgcccagat tgcgaaaaag cgccgcgcga
     1801 ggcatttttt ctttgccctg cagagcgtcg atggtgccgc cgttgagcag gtaaatatcg
     1861 agcttgccgt cgttgtcgaa atccagcacg gcgaccgcgc cggaagtgct ttcgggcaga
     1921 taatttttcg ctttatcgcc gcccgtctgg acaaagtttt tgagcgcggt ctgcgtcgtc
     1981 acgtcttcaa aaactttcgg cgcgcccgct tcggtaattc cggtcgtgcg acgcgaagaa
     2041 taagtgcggg cgtcgccggt cgaagaaccg cccgacatcg tattctgcgc gctgacgccg
     2101 aacgcgagat ttaaaagcag aaaaaaaata aagccttttt gaaacataaa atcattttaa
     2161 ccggaaaccg tttttagtaa accggaacgt taaaaatccc tcgcatcgac gcgccgtttc
     2221 gcttcgtcga tcagctcgtt gtttttctca tacgaaagat gctcgcccgc ttccgcattt
     2281 tccgattgcg gcggatacag gtgattttga taaaccggat aagcttcggt gcgcagatgc
     2341 gcgagcatat gatgcccgcg ccgcgtgcct cgctcgtgac gcagcgccga aatatcgacg
     2401 ggcgcgacga cgattctctc gcccggtccg ggcgaagcgt cggcgagcaa tctgccttca
     2461 aaatcgacaa tctgactgcc gcccgaccac gaataaggcg gataatgtct gaggctcgcg
     2521 gcttgattgg cggcgacgac ataagccata ttttcaatcg cccgcgcccg gtttatcacc
     2581 gtccaccaat ccatcggcgc ggtcgcgttc cacgggtcca tataagccga aacgcggacg
     2641 agaatctcag cgccgtttgc cgccagttgg
//
LOCUS       DASWNE010000986         1819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_491949, whole genome shotgun sequence.
ACCESSION   DASWNE010000986 DASWNE010000000
VERSION     DASWNE010000986.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491949"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            403..1776
                     /gene="allB"
                     /locus_tag="VGB68_19060"
     CDS             403..1776
                     /gene="allB"
                     /locus_tag="VGB68_19060"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR03178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HEY0051404.1"
                     /translation="MDSSDLIIRSRRVVLPDAIAPASVHIRDGVIAALGEWSEAPGRT
                     PFVDAGEAVVMPGLVDAHVHVNEPGRTEWEGFATATRAAAAGGVTTLVDMPLNSIPPT
                     TTLAGFKEKLAAARGQCQTDVAFWGGVIPGNTRELEPLLNAGVRGFKCFLVHSGVDEF
                     PHVTEANLLEAMPELARLDSVLLVHAELAEPIENAAAELKTGNPKDYQTFLKSRPRAS
                     ENEAVALMIRLCRRTGARVHIVHHSSSDALPILRAARAEGLPLTVETCPHYLTFAAEE
                     IPAGATHFKCCPPVRERENREKLWEALADGVIDMVVSDHSPCTPNLKLPETGDFLEAW
                     GGIAALQFSLPVIWTQAEKRGFGLRELTRWLSRAPAELAGLDKRKGRLAIGYDADIVI
                     WHPEKEFQIVPEIIHFRHKLTPYSGMNLRGVVEATYVRGAKVYENGKFGTKTAGKLLT
                     DSEHLDV"
BASE COUNT          404 a          506 c          521 g          388 t
ORIGIN      
        1 caagctcgga acgacggcgg cgacgaaagc tccggctccg agttctttta tgcttgaaaa
       61 aggtaatggg aatagttcgc cgggattgtt ttgtcttaag ctcggagaaa tgccggccgc
      121 cgcctttccg gtgtgattaa agcgcgtcta attatcgccg ttcccttttc caaatcaata
      181 tcatttccat tgaagcgaaa gatattgatt taatcggcag cccgtcgcaa agcaaaggaa
      241 aacgtcgttg cacatcactg cgcattccgc cgacacgctc cgtaaccgtc gcgtttccgc
      301 ctttatcgcc ttttcaaaaa ttacaccggc aaataaaaaa tcgcttgtaa cattgcgaat
      361 ttgcttatat actccgcaaa cagcgttgat ttcagacgag atatggacag ttcagaccta
      421 attattcgca gccgccgcgt cgtcctgccg gacgcgattg ctccggcttc ggtgcatatc
      481 cgcgacggcg taattgccgc tctcggagaa tggagcgaag cgccgggacg aacgccgttt
      541 gtcgatgccg gagaggcggt ggtgatgccc ggactggtgg acgcgcacgt tcacgttaac
      601 gaacccgggc gcaccgaatg ggaaggcttt gcgacggcga cacgcgcggc ggcggctggc
      661 ggcgtgacga cgctcgtcga tatgccgctc aacagcattc cgccgacgac gacgctcgcc
      721 ggatttaaag aaaagctggc ggcggcgcgc gggcaatgtc aaacggacgt cgctttctgg
      781 ggcggcgtca taccgggcaa cacgcgcgaa ttggagccgc ttttaaacgc cggcgtgcgc
      841 ggcttcaaat gttttctggt gcattcgggc gtcgatgaat ttccgcacgt caccgaagcg
      901 aatctgctcg aagcgatgcc ggagctggca agactggatt cggttttgct cgtccacgcc
      961 gaactcgccg agccgattga aaacgccgcc gccgaattaa aaaccggcaa tccgaaagat
     1021 taccaaacct ttctgaaatc acgcccgcga gcctcggaaa acgaagccgt cgccttgatg
     1081 attcggctct gccgccggac cggcgcgcgc gtccatatcg ttcatcattc ttcttccgac
     1141 gcgctgccga tactgcgggc ggcgcgcgcg gaaggcttgc ctttgacggt cgaaacctgt
     1201 ccgcattatc tgaccttcgc ggcggaagaa attcccgcgg gcgcgactca tttcaaatgc
     1261 tgcccgccgg tgcgcgagcg cgaaaatcgc gaaaaactct gggaagcgct ggcggacggc
     1321 gtaatcgata tggtcgtctc cgaccattcg ccctgcacgc cgaatttaaa actgccggag
     1381 acgggcgatt ttctcgaagc ctggggcggc atcgcggctt tgcagttcag cttgcccgtg
     1441 atttggacgc aggcggaaaa gcgaggtttc gggctgcgcg agctgactcg atggctgtcg
     1501 cgagcgcccg ccgaattggc tggtctggat aaacgcaaag gcaggctggc aatcggctac
     1561 gacgccgata ttgtgatttg gcatcccgaa aaagagtttc aaatcgttcc ggaaatcata
     1621 catttccggc acaagctcac gccttattcg ggaatgaatc tgcgcggcgt cgtcgaagcg
     1681 acatacgtgc gcggcgcgaa agtttacgag aacggaaagt ttgggacgaa aaccgccggt
     1741 aaattattga cggattccga gcatctcgac gtctgatttt cttctgaaca ttcacaaata
     1801 tcgttctgcc ggtatgaaa
//
LOCUS       DASWNE010000987         2523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_67688, whole genome shotgun sequence.
ACCESSION   DASWNE010000987 DASWNE010000000
VERSION     DASWNE010000987.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67688"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..2493
                     /locus_tag="VGB68_19065"
     CDS             214..2493
                     /locus_tag="VGB68_19065"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEY0051405.1"
                     /translation="MTDLEAYKDKFSDSGRRVLDGAMNESRRRDQNYISIEHILHALA
                     DEEAELFNSTMRDLSIDPRTVRLLLEKRLESTRAHTGKGFRIAPETTDLFKRSMERAR
                     SQGRRVIDGSDIFYVVSTDERSLLNDILQNLGVPSDEVASTARMRIKTREQEEERTRR
                     KYELPSYLKHFGVSLNKLARQDKIPPTIGREMEIRQMLEILSHRERSNSPMLVGEPGV
                     GKTAVVEGLARLIELEPEKVPERLRNAHIVQLQMGGLVAGTMLRGMFEERIKGIIDEV
                     KEKSDIILFIDEAHTIIGAGAALGTSSDAANMFKSALARGELRIIGATTITEYKEYIG
                     EDEALARRFRLVKVDEPTISETKEILLGLRPRLEKNYSVTISDEAINTALEMAPKYIR
                     NLHLPDKAIGWLDTASVKVEINEPTSAVVKAEHIIDVISQESRIPKDMIFRDTTDRFS
                     HMEEILGQRVIGQKDAVKAVAQRLRLNKGPLKENHYKPDGVLLFLGPTGVGKTELAKA
                     VAEFMFGDEGKMVRIDMSEYGDGTVGIEKLIGMPRGIVGSERGGILTEQLRENPYTVL
                     LLDEVEKASPYLMNLFLQAFDEGWMTDGRGKKVYLSDAIVIMTSNLGSENFKKYEKPL
                     GFGQKTLGDIREIKGAAMKAAEQRFSPEFRNRIDEIVVFSPLMMDEVREIARLYLGKL
                     KRQMERQGKLVEITEKAIELVTEKGYSPAYGARFLKRFIDEKVKLPITNEWKLAMRFN
                     VDAEDGEIVVKAIDAFSLN"
BASE COUNT          662 a          608 c          686 g          567 t
ORIGIN      
        1 ctgaaagacg caatgcgata tgagtgtttg ttttcagaca gactttcttg tgtttattca
       61 ataaaataac gagatgaatc ggcaggttaa tgaaaagcgc taatctgccg atttgtttct
      121 tgtcttttgc tactttgaaa atgttacaat gtttccgggc ataaattgtc gtccacacgg
      181 catctaaagt ttcaggagca gtaagggaaa tttatgacag atttagaagc ctataaagat
      241 aaattcagcg acagcggcag gcgtgttctg gacggcgcga tgaacgaatc gagacgccgc
      301 gaccagaatt atatttctat cgaacacatt ctgcacgctc tggctgacga ggaagccgag
      361 ctttttaatt cgactatgcg cgacctttcg attgacccgc gcacggttcg gcttttactg
      421 gaaaaacgcc ttgaaagtac ccgcgctcac acgggcaaag gctttcgcat tgcgcctgaa
      481 acgaccgacc tttttaaacg ctcgatggag agggctcgct cgcagggtcg ccgcgtgatt
      541 gacggaagcg atatttttta tgtcgtctcg actgacgagc gcagtctttt aaacgatatt
      601 ttgcaaaacc tgggagttcc ttcggacgaa gtggcttcga cggcgcggat gcgcatcaaa
      661 acgcgcgagc aggaagaaga acgcacgcgc aggaaatacg aattgccatc atacttaaaa
      721 cattttggcg tcagccttaa taagctcgcc cgccaggaca aaattccgcc gaccatcggg
      781 cgcgaaatgg agattcgcca gatgcttgag attctctcgc accgcgagcg ctcgaattcg
      841 ccgatgctcg ttggcgagcc gggcgtcggc aaaaccgcgg tcgtcgaagg cttggcgcgt
      901 cttatcgagc tcgaaccgga aaaggttccc gaacgcttgc gcaacgccca catcgtgcag
      961 ctgcaaatgg gcggactcgt cgccggaacg atgctgcgcg gaatgtttga agagcgcatc
     1021 aaaggcatca tcgacgaagt caaggaaaag agcgatatta ttcttttcat cgacgaggcg
     1081 cacacgatta tcggcgcggg cgcggcgctc ggcacgagtt cggacgcggc gaatatgttc
     1141 aaatccgcat tggcgcgcgg cgagctgcgc attatcggcg cgacgacgat caccgaatac
     1201 aaggaataca tcggcgaaga cgaagcttta gcccgccgtt ttcgtcttgt caaagtcgac
     1261 gagccgacga ttagcgaaac caaggaaatt cttctcggct tgcgtccgcg actggagaaa
     1321 aattattcgg tgacgatttc ggacgaagcc atcaacacgg ctctggaaat ggctccgaaa
     1381 tacattcgca atctgcatct gcccgataag gcaatcggct ggctcgacac ggcttcggtc
     1441 aaagtcgaaa tcaacgagcc gacttcggcg gtcgtcaaag ccgaacacat tatcgacgtt
     1501 atctcgcagg aatcgcggat tccgaaagat atgattttcc gcgacacgac cgaccgtttc
     1561 tcgcatatgg aagagattct cggtcagcgc gtcatcggtc agaaagatgc ggtcaaagcc
     1621 gtcgcgcaaa gattgcgttt gaacaaagga cctttgaagg aaaatcatta taaacccgac
     1681 ggcgttcttt tgtttttagg accgaccggc gtcggcaaaa ccgagctggc gaaagcggtt
     1741 gccgagttta tgttcggcga cgaaggcaaa atggtgcgca tcgatatgtc ggaatacggc
     1801 gacggaacgg tcggcatcga aaagctgatc ggtatgccgc gcggaattgt cggctcggag
     1861 cgcggcggaa ttctgaccga acagcttcgg gaaaacccgt atacggttct gctgctggac
     1921 gaagtcgaaa aagcttctcc gtatttaatg aatttgtttt tgcaggcttt tgacgaaggc
     1981 tggatgacgg acgggcgcgg caagaaagtt tatctttccg atgccatcgt cattatgact
     2041 tccaatttag gctccgaaaa ctttaagaaa tacgaaaaac cgcttggttt cgggcagaaa
     2101 actctgggcg acatccgcga gatcaaaggc gcggcgatga aagccgccga acagcgcttc
     2161 tcgccggaat ttcgaaatcg tattgacgaa atcgtcgtct tttcgccgct catgatggat
     2221 gaagtgcgcg agattgcgcg tctttatctg ggcaaattaa aacgccagat ggaacgtcag
     2281 ggcaagctcg tcgaaatcac ggaaaaagcg attgagctgg tcaccgaaaa aggctacagc
     2341 ccggcttacg gcgcgcgttt cctgaagcgt tttatcgacg agaaggtgaa gcttccgatt
     2401 acgaacgaat ggaagctggc gatgcgcttc aatgtcgatg ccgaagacgg cgagatagtc
     2461 gtcaaagcga tagacgcttt cagcttaaac taatttcaaa gtcaaattaa tttattataa
     2521 agc
//
LOCUS       DASWNE010000988         2519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_316497, whole genome shotgun sequence.
ACCESSION   DASWNE010000988 DASWNE010000000
VERSION     DASWNE010000988.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316497"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..715
                     /locus_tag="VGB68_19070"
     CDS             26..715
                     /locus_tag="VGB68_19070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEY0051406.1"
                     /translation="MTENLAERLAKVKKQIAAAAEKAGRSAAEITLIAVSKTHPAAVL
                     SEAIEAGASALGENKVQEAERKIPEIGREKAQWHLIGHLQSNKARKAVRLFDFIHTLD
                     SVELAGRLERICIEENVSALSVFVQVDLAREATKSGIDEKDLPQLIEFLRACRHLKLE
                     GLMTLPPFFEDVEKARPFFAKLRGWRDRLLPEGKLSMGMSHDFEAAIEEGATHVRVGT
                     AIFGERNYTNR"
     gene            736..1329
                     /locus_tag="VGB68_19075"
     CDS             736..1329
                     /locus_tag="VGB68_19075"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEY0051407.1"
                     /translation="MLTRLIYPVISTIVLYAMLIFIALLTLRLIFNYSDPNPFGKIGR
                     FSYHLKKRTDRFVYPAARFLANFRVDTRLAPIVTILISAILAYFALQIIGNTFFIIDV
                     LKEGIANGNVKWIIGILLYALLSAYVLLIFIRFISSWFVFTKNTFFGFVKKVTDPVLI
                     PFQRLIPPIGMFDISAMILLILIQFLQIMVLRAFGIS"
     gene            1326..1622
                     /locus_tag="VGB68_19080"
     CDS             1326..1622
                     /locus_tag="VGB68_19080"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEY0051408.1"
                     /translation="MIQPAEKNNALVFTVKVVPRASKSEIVGSLDGALKIRIAAPPVD
                     GAANAELVRLLSKTFGVSKSAIEILGGQSARTKQVKISGVEAEDFLKITGVAIS"
     gene            complement(1701..1922)
                     /locus_tag="VGB68_19085"
     CDS             complement(1701..1922)
                     /locus_tag="VGB68_19085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051409.1"
                     /translation="MKKAIYHAENLARNSRFSHTPVMRARFNQSETKGKSVCRKNQPR
                     RQLRFRACWQIFYAPGQQFKTGIIRTRNG"
     gene            1962..>2519
                     /locus_tag="VGB68_19090"
     CDS             1962..>2519
                     /locus_tag="VGB68_19090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY0051410.1"
                     /translation="MSGHSKWHTIKHKKGALDAKRGKIFTKLIKEITVAARTGGTGDV
                     DANARLRKAVNDAKAQNMPIDTITRAIKRGTGELEGVNYEEITYEGYGVGGVAVMVET
                     MTDNRNRTVAELRHLFSKNGGNLGESGSVAWMFDKKGYIVVDKDSKAEDELFEIALEA
                     GADDMQAEGDVIEIYTAPENFEPVHE"
BASE COUNT          646 a          607 c          661 g          605 t
ORIGIN      
        1 cgccgtttct tttatcatct agacaatgac ggaaaatttg gctgagcggc tggcaaaagt
       61 taaaaaacaa atcgcggcgg cggcggaaaa agcgggcaga agcgcggcgg aaatcacttt
      121 gattgcggtc agcaaaactc atccggcggc ggttctgagc gaggcgatcg aggcgggcgc
      181 aagcgctctg ggcgaaaata aagttcagga agccgagaga aaaatcccgg aaatcgggcg
      241 cgaaaaggcg caatggcatc ttatcgggca tctgcagtcg aacaaggcgc gcaaagccgt
      301 cagacttttt gactttatcc acacgctgga ttcggtcgaa cttgccgggc gtctggagcg
      361 tatctgtata gaagaaaatg tttcggcgct ttccgttttc gtgcaggtcg atttggcgcg
      421 ggaagcgacc aaatcgggaa tcgacgaaaa agatttgccg caattaatcg aatttttgcg
      481 cgcgtgccgt catctaaagc tcgaaggctt gatgacattg ccgccttttt ttgaagatgt
      541 cgaaaaggct cgaccgtttt tcgctaaatt gcgcggctgg cgcgaccggc ttctgcccga
      601 aggaaagctt tcgatgggaa tgagccacga tttcgaagcg gcgattgaag aaggcgcaac
      661 gcacgtccgc gtcgggacgg cgattttcgg cgagagaaat tacacgaatc gctgagagag
      721 cgtttcatca aaattatgct aacgagatta atttatccgg ttatcagcac catcgttctt
      781 tacgcgatgt taattttcat cgcgcttctg actctgcgtc tgatttttaa ttattccgac
      841 ccgaatcctt tcggcaaaat cggcagattt tcctatcatc tcaaaaaacg aaccgaccgt
      901 tttgtttacc cggcggcgcg atttctcgcc aattttcgcg tcgatacgcg gctcgcgccg
      961 attgtcacga ttttgatttc cgcgattctg gcttatttcg cgctccagat tatcggaaac
     1021 acctttttta ttatcgacgt tctgaaagag ggaatagcga acggaaacgt caaatggata
     1081 atcggaatcc tgctttacgc cctgctcagc gcttatgttt tgttaatctt tatacgcttt
     1141 atctcgtctt ggttcgtgtt taccaaaaat acgtttttcg gattcgtcaa aaaagtgacc
     1201 gacccggttt tgattccttt tcagcgcttg attccgccaa tcgggatgtt tgatatttcg
     1261 gcgatgattc tcttgatttt aatacagttt ctgcaaatta tggttttgcg cgcgtttgga
     1321 atttcttgat tcaaccggca gagaaaaata acgctcttgt ttttaccgtc aaggtcgtcc
     1381 cgcgcgcctc gaaaagcgaa attgtcggaa gcctcgacgg cgctctgaaa attcgaattg
     1441 ccgcgcctcc ggtcgacggc gcggcaaacg ccgaactcgt caggcttttg tcgaagacgt
     1501 tcggcgtttc caaaagcgca atcgaaatcc tcggcggtca gagcgcccga acaaaacagg
     1561 tgaaaatttc gggcgtcgag gcagaggatt ttttaaagat aacgggcgtc gcaatttctt
     1621 agacacaagc cttttcatca aaaacggttt gaaaaaaagc cggaagcggc agcccggttt
     1681 tcggacataa aaaaataaaa tcacccgttg cgcgtccgaa ttatgccggt tttgaattgt
     1741 tgtcccggcg cataaaaaat ctgccagcaa gcccgaaaac gcaactgccg gcgcggctga
     1801 ttttttcgac atacgctttt tcctttcgtt tcgctttgat taaaacgcgc gcgcatcacg
     1861 ggcgtatgag aaaagcgcga gttgcgcgct aaattttcgg cgtgatatat tgcctttttc
     1921 actaattctt aaaattcagt caataaagag gaaaaacaag aatgtcggga cattccaagt
     1981 ggcataccat caagcacaaa aaaggcgcgc tcgacgccaa gcgcggcaag atttttacca
     2041 agctgattaa agaaatcacc gtcgcggcgc gcacgggcgg caccggcgac gtcgatgcca
     2101 acgcgcgcct gcgaaaggcg gttaacgacg ccaaggcgca gaatatgcct atcgatacga
     2161 ttacgcgcgc catcaagcgc ggcacgggcg aactggaagg cgtcaattac gaggaaatca
     2221 cgtatgaagg ctacggcgtc ggcggcgtcg ccgtgatggt cgaaacgatg accgacaatc
     2281 gcaaccggac ggtcgccgaa ctgcgtcatc tgttttcgaa aaacggcggg aatctcggcg
     2341 aatccggctc ggtcgcctgg atgttcgaca aaaaaggcta tatcgtcgtg gacaaagatt
     2401 caaaggcgga agacgagctt tttgaaatcg ctctggaagc gggcgcggac gatatgcagg
     2461 cggaaggcga cgttattgaa atctacaccg cgccggaaaa tttcgagcca gtccacgaa
//
LOCUS       DASWNE010000989         5735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_668645, whole genome shotgun sequence.
ACCESSION   DASWNE010000989 DASWNE010000000
VERSION     DASWNE010000989.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668645"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1382)
                     /locus_tag="VGB68_19095"
     CDS             complement(<1..1382)
                     /locus_tag="VGB68_19095"
                     /inference="COORDINATES: protein motif:HMM:NF026968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEY0051411.1"
                     /translation="MKIYISGLYSGTNPQPGVGIARSIRAAYPQAKLIGVEYSNRCSG
                     IHWQDFDEIWLQRPWDELNLTTHAAEIKKVLDEGGLWISSIDLEILWLANLFPEGHRN
                     LLTPPLAAMNQVGKPAIPAHAGLPVKIPTFVTTEISDWDLHAFCREHDWKVWLKGPYY
                     EAVKIGGWAHFEEMRNVLSSAWATDKLFLQAHVSGYEESITISAYEGKLLECVRMRKR
                     DLTEMSKTWAGDVSDVPEEISEPLREIIKNINWTGGAELEMVRDADNQLWLLEWNPRF
                     PAWIHGSTITGRNIPGSLIEGATGIPAQETIATGEEFTRVVLEVPVRPEFPLPPLPEP
                     LGGRIGHSLKHPSGLLQFANRLHKLEFDHDGNGSEGVNGKPAENQTATVPTTFIDDLQ
                     SLDFSQMQTPASVFLKNTAKEYFQKAAALAADLTTEQIKVNNAYSIKTNPDKRLIKLA
                     LKSGFYAEAI"
     gene            complement(1416..2162)
                     /locus_tag="VGB68_19100"
     CDS             complement(1416..2162)
                     /locus_tag="VGB68_19100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051412.1"
                     /translation="MAGATETNGEILSEEKLAEDTLVFEPVDSRIVYPSYKSFDEFID
                     VERLRALDGYILQRIKRRLNAPQDDYKFYTGPYKLNDKIPDRPGSRMIYLAYSEQPDS
                     YFDLDKTELWHPTQAANEFALLMDFIRTLPFQATGRMMIMYDDAPREVPAHRDHVETD
                     VCHEFVWFRTNTKKPFYMFNYQTGEKKYVETYTAWFDSVNQFHGSDGHDGLSFSIRVD
                     GKFTDEFREKIPKPEFNLASTPSYWATVAS"
     gene            complement(2205..2942)
                     /locus_tag="VGB68_19105"
     CDS             complement(2205..2942)
                     /locus_tag="VGB68_19105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051413.1"
                     /translation="MEDRTDENAFVTNLKQAKFDSRITYPCYKSFDEFIDVERLRALD
                     DYIIERINLHIKAEKDDYFLNLHRLDEEMPYQPGVREIWLTRTKPDAPYDYINLDNSD
                     LWERTEAAEEFADLMSFIETLPFKETGRMLIIYDNAGKEVPPHRDHLKTEICNEFIWF
                     RTNLIKPFYVLNHLSGAKKYVESYSAWFDAVNQFHGSEAVEALSFSIRVDGKFTDEFR
                     EKIPKPEFNLASTPSLWASLERQKGQL"
     gene            3194..4168
                     /locus_tag="VGB68_19110"
     CDS             3194..4168
                     /locus_tag="VGB68_19110"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY0051414.1"
                     /translation="MIKTSFLNPQKLPLVIEPAVDKENEADLDLLVRTARAQPDFFRE
                     KLLTHGALLFRGYQARSVSDLERFVREFSGGEELFSYAGGVSPRNPLGSGGGVYTSTE
                     YPAQYALSLHNELSYADLYPRHLYFCCLTPAETGGETTLGDSRRILQNLDAEIVELFK
                     NKKNCYVRNLQSGPGDGYSWQEAFETVDKETVEDCCRKIGAEFQWQAGDGLRLRQIRP
                     AIINHPVTGEEVWFNQADGFHPSNLDAETYQMLIAAMSEDEFRLNVFFGDGSPIGLET
                     LAHVRAVLQSETIPHRWQAGDLLILDNILAAHGRLPFTGARKIALAMT"
     gene            complement(4246..4806)
                     /locus_tag="VGB68_19115"
     CDS             complement(4246..4806)
                     /locus_tag="VGB68_19115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051415.1"
                     /translation="MKTKFLFSALLICLSGFGLAASAQTKQPKTVRDFFMLLPAKYFS
                     LDCCIDKNARQGKEEYLELYLSVEDTANGYMSGGDGAQDGFAMALFKRSNGTYLIAFY
                     TYGEGGVEDTSWTVFLDYKNGKWTDVSRAVVPNYNARKYIYQLPREGTTIGVFEKDET
                     GEDFYKGKKLYDLIWQNDKFSRSGKR"
     gene            5070..>5735
                     /locus_tag="VGB68_19120"
     CDS             5070..>5735
                     /locus_tag="VGB68_19120"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEY0051416.1"
                     /translation="MNRRLSATAFALIFCSLIFIRTTAAQSEKDIVPTREMDRINWME
                     FKETVPSKIQTVLLPTGTLEPHGVINNGADNTAPTAMARAVARRANAMIAPTLPYGIT
                     GSMADYPGAFTITEAAYRPFVKQILEGLARTGFKNIIILNGHGGAQTAVLQSVAAEVS
                     AERRVRTLVINWWSFASDETKAVFGEDGGHAGWNETAYIQAIDPTLVKKERYSADLAT
                     AYPA"
BASE COUNT         1338 a         1499 c         1438 g         1460 t
ORIGIN      
        1 ctgatggctt cggcgtaaaa accggatttg agcgccagct ttatcagccg tttgtccgga
       61 ttggtcttga tgctgtaggc gttgttgacc ttgatttgct cggtcgtcaa atccgcggcg
      121 agcgcggcgg ctttctgaaa atattctttc gccgtgtttt tcagaaatac gctggcgggc
      181 gtctgcatct gcgaaaaatc cagcgattgc aaatcgtcga taaaagtcgt cgggacggtc
      241 gccgtttgat tttccgcggg cttgccgttg acgccttcgc tgccgtttcc gtcgtggtcg
      301 aattcgagct tgtgcaggcg gttggcaaac tgtaaaagcc ccgaaggatg cttgagcgaa
      361 tgcccgattc tgccgcccag aggttcgggc agaggcggca gcggaaattc cggtctaacc
      421 ggcacttcga gcacgacgcg cgtaaattct tcgcccgtcg ctatagtttc ctgcgcggga
      481 attccggtcg cgccttcaat caaactgccg ggaatgttgc gcccggtaat ggtcgagccg
      541 tgaatccacg ccggaaaacg cgggttccat tcgagcagcc acaactgatt atcggcgtcg
      601 cgcaccattt ccagctccgc gccgcccgtc cagtttatat ttttgataat ttcgcgcagc
      661 ggctcggaaa tttcttcggg cacgtcggag acgtcgcccg cccaggtctt gctcatttcc
      721 gtcaaatcgc gtttgcgcat ccgcacgcat tcgagcaatt tgccttcgta agccgagatg
      781 gtgattgatt cctcgtaacc ggagacgtgt gcctgcaaaa atagcttgtc ggtcgcccac
      841 gcgctcgaca gcacgttgcg catttcctcg aaatgcgccc agccgccgat ttttaccgct
      901 tcgtaataag gacctttgag ccagactttc caatcgtgct cgcggcaaaa ggcgtgcaaa
      961 tcccaatcgg aaatctcggt cgtcacaaag gtcggaattt taacgggcag cccggcgtgc
     1021 gccggaatcg ccggtttgcc gacctgattc attgccgcca gcggcggcgt cagcaaattc
     1081 cgatgacctt cgggaaagag attggcgagc cacagaattt ccaaatcgat gctggaaatc
     1141 cagagtccgc cttcgtccag aacttttttg atttcggcgg cgtgcgtcgt caggttcaat
     1201 tcgtcccacg gacgctgaag ccagatttcg tcaaaatcct gccagtgaat gcccgaacag
     1261 cgattcgaat attcgacgcc gatgagtttt gcctgcgggt aagccgccct gatggaacgg
     1321 gctataccga cgccgggctg cgggttggtg cctgagtaga gtccgctgat ataaatcttc
     1381 ataatttccc ttgtacgatt ctcgatataa aatttttaac ttgctacagt tgcccaatac
     1441 gagggcgtcg atgcgagatt aaattccggc ttcggaattt tttcccggaa ttcgtccgta
     1501 aatttgccgt ctacgcgaat actgaacgat aagccgtcgt gcccgtcgct gccgtgaaac
     1561 tggttgaccg aatcgaacca ggcggtgtag gtttcgacgt attttttctc gcccgtctgg
     1621 taattgaaca tataaaacgg ctttttcgtg tttgtccgaa accagacgaa ttcgtgacaa
     1681 acatcggttt cgacgtggtc gcgatgcgcc ggaacttcgc gcggcgcgtc gtcatacata
     1741 atcatcattc tgccggtcgc ctgaaacggc agcgttcgta taaaatccat cagcagcgcg
     1801 aattcgttgg cggcttgggt cggatgccaa agctcggttt tgtccaaatc gaaatagctg
     1861 tccggctgct ccgaatacgc cagataaatc atccgcgagc cgggacggtc ggggatttta
     1921 tcgtttaatt tgtatggacc ggtgtaaaac ttgtaatcgt cctgcggcgc gttcagacga
     1981 cgcttgattc tttgcagaat atagccgtca agcgcgcgca ggcgttcgac gtcgataaat
     2041 tcgtcaaacg atttatagct cggataaaca atccgcgaat cgaccggctc aaagacgagc
     2101 gtgtcctccg ccagcttttc ctcgctgaga atttccccgt tcgtttcggt tgcgcctgcc
     2161 atagtcttaa tttccgtctc tgatttattc ataaaaattt tctttcatag ctgtcctttc
     2221 tgcctctcca gagaagccca gagcgacggc gtcgaagcca gattaaattc cggcttcgga
     2281 attttttccc ggaattcgtc cgtaaatttg ccgtctacgc gaatactgaa cgataaagcc
     2341 tcgaccgctt cgctgccgtg aaactggttg accgcgtcaa accacgcgct gtaggattcg
     2401 acgtatttct tcgcgccgct caaatgattt aaaacgtaga acggcttaat caaattcgtg
     2461 cgaaaccaga taaactcgtt gcagatttcc gtctttaaat ggtcgcggtg cggcggcact
     2521 tccttgcccg cattgtcgta aataatcagc attctgcccg tttccttaaa cggcagagtt
     2581 tcgataaagc tcatcaaatc ggcaaattcc tccgccgctt cggttcgttc ccaaaggtcg
     2641 ctgttatcga gattgatata atcatacggc gcgtccggct tggtgcgcgt cagccagatt
     2701 tcgcgcacgc cgggctgata cggcatctcc tcgtccaacc ggtggagatt gagaaaataa
     2761 tcatcctttt cagccttgat atgaaggttt attcgctcga tgatgtaatc gtcaagcgcg
     2821 cgcaggcgct cgacgtcaat aaattcgtca aaagatttat agcaaggata ggtgatgcgc
     2881 gagtcaaatt ttgcctgttt caaattggtg acgaaagcgt tttcatcagt gcgatcttcc
     2941 acaattttaa aaacctcctt gttcttttaa tttctcaagc cttttaaatc tctcggggct
     3001 taatattttc atccgcggcg cagttttcga aatccgtgaa atatcaacac tatatgcgcg
     3061 catatgtaat ttataattta taaaatatcg tcattttaaa acttcgtctg ttcgatagca
     3121 gacaaatata atttaaaaaa cccggcataa taattttcgg cttggttttt attaataaaa
     3181 gtctttaaaa aatatgatca agacgagttt tctcaatcct caaaaactgc cgctggtgat
     3241 tgaaccggct gtcgataaag aaaacgaagc cgatttggat ttactcgtcc ggacggcgcg
     3301 cgcgcagccg gatttttttc gggaaaagct tttgacgcac ggcgctttgt tgtttcgcgg
     3361 ctatcaggcg cgcagcgttt ccgatttaga gcgtttcgtt cgcgaatttt ccggcggtga
     3421 agaattattc agctacgcgg gcggcgtatc gccgcgcaac ccgctcggca gcggcggcgg
     3481 cgtttacact tcgaccgaat acccggcgca gtacgcgctt tcgctgcaca acgaattgtc
     3541 ttacgccgac ctttacccgc gccatcttta tttctgctgc ctgacgccag ccgaaaccgg
     3601 cggcgagacc acgctcggcg acagccgacg gatactgcaa aacctcgacg cggaaattgt
     3661 ggaattgttc aaaaataaaa aaaactgtta cgtccgcaat ctgcaatccg gtccgggcga
     3721 cggttactcg tggcaggaag cgtttgaaac cgttgataaa gaaacggtcg aagattgctg
     3781 ccgtaaaatc ggcgcggagt ttcagtggca ggcgggcgac ggcttgcgtc tgcggcagat
     3841 tcgaccggca atcatcaatc acccggtgac aggcgaagaa gtctggttta atcaagccga
     3901 cggttttcat ccgtccaatc tggacgcgga aacctatcaa atgctgattg cggcgatgag
     3961 cgaagacgaa tttcggctca acgttttttt cggcgacggc tcgccgattg gtttggaaac
     4021 gctcgcacac gttcgcgccg tcctgcaaag cgaaacgatt ccgcaccgct ggcaggcggg
     4081 cgacctttta attctggata atattttagc cgcgcacggt cgtctgccgt ttaccggagc
     4141 gcgaaaaatc gcgctggcaa tgacttagaa aagcggtaaa cggtaagcgg taagtggtaa
     4201 gttcgtttat ttccgattgc agttttatca attgcaactt accgtttacc gtttaccgct
     4261 tcttgaaaac ttatcgtttt gccagattaa atcgtaaagc tttttgcctt tgtagaaatc
     4321 ctcgcccgtt tcgtcttttt cgaaaactcc gatggtcgtg ccctctcgcg gcagttggta
     4381 gatgtatttc cgagcgttgt aattcggcac gaccgcgcgc gagacgtccg tccattttcc
     4441 gtttttgtaa tccagaaaaa ccgtccacga cgtatcttcc acgccgcctt cgccgtaagt
     4501 gtaaaaggcg atgagatacg ttccgttcga gcgtttgaaa agcgccatcg cgaaaccgtc
     4561 ctgcgcgccg tcgccgccgc tcatataacc gtttgccgtg tcttccacgc tcagatagag
     4621 ttccagatat tcttccttgc cttgacgcgc gtttttgtcg atgcagcaat cgagactgaa
     4681 atatttcgcc ggaagcagca taaaaaaatc tcgcacggtt ttcggctgct tcgtctgcgc
     4741 cgacgctgca agcccgaatc ccgataaaca aattaataat gccgaaaata aaaatttcgt
     4801 tttcataatt tctcctttta taaactcaaa agccgaatag aaaagaccgg aatcagcccg
     4861 cgcttttgcc tgtttttaag tgacaatcgc tgattttata caaccagccg ctgtatttga
     4921 gacagacagt cactcatttt aaagatacag acgggcattt cagagcgtca gtcggtcatt
     4981 ttaaactgca aattgaaaag attgcggctg tattgcacaa aatctgccgg taagcgaaga
     5041 taaattcaaa aaatcccaaa ggagctcaaa tgaatcgaag actttcggca accgcgtttg
     5101 cccttatttt ctgctcgctg atttttatcc ggacgaccgc cgcgcaaagc gaaaaagaca
     5161 ttgtgccgac gcgcgagatg gacagaataa actggatgga atttaaggaa accgtgcctt
     5221 ccaaaattca gaccgttctg cttccgaccg gaactttaga gccgcacggc gttatcaaca
     5281 acggcgcgga caacaccgcg ccgacggcga tggcgcgcgc cgtcgcccgc cgcgccaacg
     5341 cgatgattgc gccgactctg ccttacggca ttaccggctc gatggctgat tatccgggcg
     5401 cgtttacgat taccgaagcg gcttatcgcc cgttcgtcaa acaaattctc gaaggcttag
     5461 ccagaaccgg attcaaaaac attatcattt taaacggtca cggcggcgcg cagaccgccg
     5521 ttctgcaatc ggtcgcggcg gaagtttccg ccgagcgacg agtgcgcacc ctggtgataa
     5581 actggtggtc gtttgcctcg gacgaaacaa aagcggtttt cggcgaagac ggcggtcacg
     5641 cgggctggaa cgaaacggcg tatatccagg cgattgaccc gacgctggtg aaaaaagaaa
     5701 gatacagcgc cgatttggcg accgcctatc cggcg
//
LOCUS       DASWNE010000990         2434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_702697, whole genome shotgun sequence.
ACCESSION   DASWNE010000990 DASWNE010000000
VERSION     DASWNE010000990.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702697"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(274..1392)
                     /locus_tag="VGB68_19125"
     CDS             complement(274..1392)
                     /locus_tag="VGB68_19125"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0051417.1"
                     /translation="MSRVKFSFFACGLLLIFTTSLWAQSGNTRPAQPTPTPPEQIERV
                     ETEEIRLNISAFDANGRFISDLRIEDLVINEDGRLHQATSVRRTPANVLILLDVGNEI
                     AYAKRRAATSAAASGLVNALGENDSLAVMQYGDKVEILSEWTKDKAAVLNVLNDKKLG
                     YGKRSVFNQALATAVDFFRKTPLENRHLVLISDGVDTFDDLRAKNLAIKNLLSSDINV
                     HVISYTKLQQNAITVPKTLTGGGSRPRTNLPPGAEPPIHGTTPTFPIATINLDREMIR
                     KRQEQIARLKSSEQLLATIAEDTNGEIFLPETVEEMVDKTAQLAKNIDSQYVVTYTPK
                     RALNQADDGEVRLIEVSSRRPGIDVQGRRKLVVINSKN"
     gene            complement(1470..>2434)
                     /locus_tag="VGB68_19130"
     CDS             complement(1470..>2434)
                     /locus_tag="VGB68_19130"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY0051418.1"
                     /translation="PPLLKKAMHDVEIIALANNIPLENFALIEGDITRKNLGISDEDF
                     ETVQHETTDVYHLAAIYDLAVPRDIALLINVRGTKNVNEFVQSLPNLRRYNYISTCYV
                     AGKRRGEILETELEHDAGFRNFYEETKYLAEIEVEKLKSELPVTIFRPSVVVGDSRTG
                     ETAKYDGIYYLIRYLRKFPAFLRFINVGNSSVRLNLVPVDFVVEAIAALAEDENATGK
                     TLALADPNALTTEQLFDVIIEALTGKKSLIKPPPKLVENSLLLSISPALTGLPHSGVP
                     YFFLSQTYDTSVAGELLSAHGISCPDFPSYVKNLLGFVEKNPSL"
BASE COUNT          572 a          602 c          608 g          652 t
ORIGIN      
        1 cagcggaaat ttaatagcag tatttactct ggaagcagcc cgaaacgtct cgactaaatt
       61 tgacggaaat tttttaatta ttaccgacga ttgcggaagg cttctggctt acgattataa
      121 aaatcgaacg ctgcgacaaa atttacgcct gtaaaatcag tactcaggtg attagatatt
      181 tcgtaaatat aaattcgtta taaaaatact ctcaaccgtc gtgaaaacga actttttaat
      241 tgccgcttca gcgttttttg aaatgtttcg attttagttt ttactgttta tgacgacgag
      301 ttttcggcga ccctgaacgt cgattccggg gcgtctggaa gaaacttcaa tcaggcgtac
      361 ttcgccgtcg tcggcttggt tcagcgcgcg tttcggcgtg tatgtgacga cgtattgcga
      421 atcgatattt ttcgccagct gcgcggtttt atcgaccatt tcctcgacgg tttccggcag
      481 gaaaatctcg ccgttggtgt cttcggcaat cgtcgccagg agctgttcgc tgctttttag
      541 ccgggcgatt tgctcttgtc gcttgcgaat catttcgcgg tcgagattaa tcgtcgcaat
      601 cggaaaggtc ggcgtcgtgc cgtgaatcgg cggttcggcg cccggcggca gattggttct
      661 cggtcggctt ccgccgcccg tcagagtttt aggaacggta atcgcatttt gctgaagctt
      721 tgtgtagctg atgacgtgaa cgttgatgtc cgacgacaat agatttttga tggctaaatt
      781 ctttgcgcgc aaatcgtcga aagtgtccac gccgtcggaa attaaaacca ggtgacgatt
      841 ttcgagcggc gtttttcgga aaaaatcgac cgccgtcgcg agcgcctgat taaagaccga
      901 gcgtttgccg tagccgagct ttttgtcgtt cagcacgttt aaaaccgccg ctttatcttt
      961 cgtccactcg gacagaatct ccactttatc gccgtattgc ataacggcga gcgaatcgtt
     1021 ttcgcccagc gcattgacca gcccgcttgc cgccgccgat gtcgccgctc ttcttttcgc
     1081 ataagcgatt tcgttgccga catccagaag aatcaaaacg tttgccggcg tccggcgcac
     1141 gctggtcgct tgatgaagcc gcccgtcttc attgataacc aaatcttcga tgcgcaaatc
     1201 cgagataaat cttccgttcg cgtcaaaagc cgagatattg agccggattt cctccgtctc
     1261 gactctttcg atttgttcgg gcggcgtcgg cgtcggctgc gccggacgcg tatttcccga
     1321 ttgcgcccaa aggctcgtcg taaaaattaa aagcagcccg caagcgaaaa acgaaaattt
     1381 gactcgtgac aaaaacataa ttttcctcaa agttattggt tatgatgttt cggcgggcga
     1441 aaaatatgct ttgacgattg taaagatttc tagagcgacg gatttttttc gacaaaaccg
     1501 agcagatttt ttacataaga cggaaaatca ggacagctta tgccgtgcgc cgaaagcagc
     1561 tcgccggcaa cgctcgtatc gtaggtctgc gaaagaaaaa aatacggcac gcccgaatgc
     1621 ggcaatccgg tcagcgccgg cgaaatcgac aaaagcaggc tgttttccac taatttcggc
     1681 ggcggtttaa tcaacgattt tttgccggtc agcgcttcga taattacgtc aaaaagctgc
     1741 tcggtggtca gcgcattcgg gtcggcgagc gccagggttt tgcctgtcgc gttttcatct
     1801 tcggcgagcg ccgctattgc ttcgacgaca aaatcgaccg gaacgagatt gagcctgacc
     1861 gaagaatttc cgacatttat aaaacgcaaa aacgcgggga attttcgcaa gtatcgaatc
     1921 agataataaa ttccgtcgta tttcgccgtc tcgcccgtcc gggaatcgcc gacgacgacc
     1981 gacggacgaa aaatcgtcac gggcagctcg ctcttcagct tctcgacttc gatttccgcc
     2041 agatatttcg tctcctcgta aaaattgcga aaaccggcgt cgtgttcgag ctcggtttcc
     2101 agaatttcgc ccctgcgttt tccggcgacg taacaggtcg aaatgtaatt gtagcgacgc
     2161 agattcggca gagactggac aaattcgttg acgtttttcg tgcctctgac attgatgagc
     2221 agagcgatat cgcgcggcac agccaaatcg taaatcgcgg cgagatgata aacatccgtc
     2281 gtctcgtgct gcacggtttc gaaatcctcg tccgaaattc ccagattttt tcgtgtaata
     2341 tcgccttcaa tcaaagcgaa attttcgagc ggaatattgt ttgcgagcgc gatgatttcg
     2401 acatcgtgca tcgctttttt gagcagcggc ggct
//
LOCUS       DASWNE010000991         6658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_136960, whole genome shotgun sequence.
ACCESSION   DASWNE010000991 DASWNE010000000
VERSION     DASWNE010000991.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6658
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136960"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VGB68_19135"
     CDS             complement(<1..357)
                     /locus_tag="VGB68_19135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051419.1"
                     /translation="MKTIKFLVVFTTIYFVFANASTAFAQAASVFTTGLTAPTKIITA
                     GQSSLLVAEAGTMTPNQGRISLVDRAGGARRTLIGGLPSGINRAEGQPAPSGPSGLKL
                     NGLKLYVTIAQGDAVLP"
     gene            493..1068
                     /locus_tag="VGB68_19140"
     CDS             493..1068
                     /locus_tag="VGB68_19140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0051420.1"
                     /translation="MEEPVRNSLQITQMLKKWSEGNQEVLDKLMPLVYEELRRQASRY
                     LRRERANHTLQTTALIHEAYLKLIDQNQVEWQNRTHFFAIASQAMRRILVDYARERNR
                     DKRGGAAENLPIEEAAFVVSPGKNIDLVALDEALNRLAEFDERQARVVELRYFSGLSI
                     DETADVLGISNVTVRRDWNMAKAWLHQQISK"
     gene            1069..5796
                     /locus_tag="VGB68_19145"
     CDS             1069..5796
                     /locus_tag="VGB68_19145"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0051421.1"
                     /translation="MTPERWQKIKDLFDVALETEPERRAGFLKNVCAGDAELRLEIEK
                     LLASFEDAGEFMEKPAVGEVAGLIIEPVSKLKNGERIAHYEILRQIGEGGMGEVYLAR
                     DTRLNRRIALKALPADLTADPLRLQRFKQEAQTASALNHPNIITIYEIGDAAGVHFIA
                     TEFVEGETLRQKMAKNDLSIADTLDIAAQTASALAVAHSTGIIHRDIKPENIMIRPDG
                     IVKLLDFGLAKLTEGGARTNAENFLTNPGMIMGTVNYMSPEQARGAAIDYRTDLWSLG
                     VVLYEMLSGVLPFQGKTVNHTLVAIMEANPSLRSQSNREIPDELEQIVAVLLSKEPHK
                     RYGAANELLADLRALRKLTESQPEKTGIRHFAAPSTRQPLSEKESVAEIGAPAIAPNN
                     LSGQLLPLVGREKELSEIINLLGREDIRLLTLTGVGGTGKTRLAQEIAAAMLAEFADG
                     VFFIALPAVRNPEFVISEIAQPLGVRDAGGKSLLDTLKDFLKARRILLVVDNFEQVLP
                     AAPLLNELLAAAPRSKMLVTSRALLRLRAEREFVVPPLDVPSDIERTAAADLLQYESV
                     RLFTKRAQAAKPALALNEENSKIIAEICSRLDGLPLAIELAAARVKILSPQAILTRLE
                     NSLSLLTGGGRDLLEHQQTMRRAIEWSYDLLDEREKILFRRLAVFAGGFTIDAAEAVC
                     SRQNAENGTEHSLSGGERISPAANRLLTTEILDLITSLIENSLLVQKETADDESRLRM
                     LEVVREFALEKLEASGESLAAKKAHAAFFLALAQKAEPEIFGEQGGKWLDRLEEEHEN
                     LRAALSRSTAAGSVTAAELAGALRNFWILHNHITEGRKWLETILEQSAEAPLAVRLKL
                     INGLGHAAGYQGDLEMARKAHEKGLAEGKAANDLRQIALSVRGLGFVAKWKDDIRTAR
                     KFYEEGLAISRRLEDRNGIAVSLTALGDLARMADDYATARPLFEESLEICKQTGNKQG
                     VVGSLNNLGAITFGEGDYAAAHSYYAEAMKAVLEFGEKISLSYALDGLAALAVQSGDF
                     ERAAQLAGIAEHLRESLGFETEPAERRFRDAYLVKLQSVLDKEIFTVFYEQGQALPLK
                     KAVALALNQSNTETGDPVKSFSAPGKNPNPVTSIAVLPFLALGFTEDEEYLGLGLADA
                     LITQLSQTRQLSVRPTSAVRSYTDGRREAAAIGKKLNVGAVLEGNLQKLGERLRVTVQ
                     LTNIESDATLWADKYNVNFTDFFEVQDQIAEQVSKALLLQLSSSEQTQINKRFTENNA
                     AYLEYLKGRHFLEKRDVDGFHKAIEHYKKAIDFDPTHALSYVGLSDVYCMFGIWGEYP
                     QEEIFPRAKAAALRALEIDEKLAEAHASLGRVSSVYEWNWEAAEKSYRRAIELNPNCL
                     NAHSWLAKVYVLRHQYDEAIEESRIAQRLDPLSPQAEVSLCAAYFYSRRYTEAVEKYR
                     KAMRMHPDFVPAIFGLGMCLAEKGEFAEAIALQEKAVELSKNHPLIEGFLAGTYAYSG
                     NIKKALESIEKFKAAEDKSLYYTIAAVYARLNEKEEAFAYLEKSFSMRVRSLSGISIE
                     PEFDNLRGEPRFRDMLRRVGLDV"
     gene            complement(5976..6593)
                     /locus_tag="VGB68_19150"
     CDS             complement(5976..6593)
                     /locus_tag="VGB68_19150"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF003296.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEY0051422.1"
                     /translation="MVEEFAFDNTEERRKFRKNGVGWVEVIVGSMFSGKSEELIRRLK
                     RANIARQKVQVFKPAIDVRYSVEEIASHSGVKHGSIPVSSAKEMLDKIDVDTEVVGID
                     EGQFFDLEVVNAVNFLANRGMRVIVAGLDQDYTGRPFEPMPQLLAVAEFITKTHAICV
                     KCGETANYSQRTVESDAVVEVGAADKYEARCRKCFVPHSDEPTNL"
BASE COUNT         1623 a         1781 c         1845 g         1409 t
ORIGIN      
        1 cggcaagacc gcgtcgccct gcgcgatggt cacgtaaagc tttaatccgt ttagctttaa
       61 gcccgacggt ccgctgggcg cgggctgccc ttccgcgcgg tttatgcccg aaggcaagcc
      121 gccgataagc gtccggcgcg cgccgcctgc gcgatcgacc agcgagatgc gcccctgatt
      181 cggcgtcatc gttcccgctt cggcgacgag caggctggat tgtccggcgg taataatttt
      241 ggtcggcgcg gtcaatccgg tggtaaacac gctcgcggct tgcgcaaaag cggttgacgc
      301 gttggcaaag acaaagtaaa tcgtggtaaa aacgactaag aatttgattg ttttcatggc
      361 acttctcctg tctgattttt ggctttcaat aattaacgcg acagtttttc ggagaaacgc
      421 tcatttttat tttttcaatt gcaaaattat tgacacccgt ctcgggcggg aatattctgg
      481 taacgaaaat ttatggaaga acccgtcaga aattctctgc aaatcacgca gatgctcaag
      541 aaatggagcg aaggcaatca ggaagtttta gataagctga tgccgctcgt ttacgaagaa
      601 ctgcgccggc aggcgtcgcg ctacctgcgc cgcgagcgag ccaatcatac tttgcagacg
      661 accgcgctga ttcacgaagc ttatctgaag ctcatcgacc agaatcaggt cgaatggcaa
      721 aaccgcacac attttttcgc gattgcgtcg caggcgatgc gccggattct ggtcgactat
      781 gcgcgggaaa gaaatcggga caaacgcggc ggcgcggctg aaaatctgcc gattgaagaa
      841 gccgcattcg tcgtttcgcc gggaaaaaac atcgatttgg tcgcgctgga cgaagcctta
      901 aacaggctcg ccgaatttga cgagcgacag gcgcgcgtcg tcgagctgcg gtatttcagc
      961 ggtttgagca tcgacgagac cgccgacgtt ttaggcattt caaacgtcac ggtcagacgc
     1021 gactggaata tggcgaaggc ttggctgcat cagcaaattt caaaataaat gacaccggag
     1081 cgctggcaaa aaatcaaaga cctattcgat gtcgcgctcg aaaccgagcc ggaaaggcgc
     1141 gcgggttttc ttaaaaacgt ctgcgcgggc gacgccgagc tgcgtctgga gatagaaaaa
     1201 ctgctggctt cattcgaaga tgccggtgaa tttatggaaa aaccggcggt cggtgaagtc
     1261 gccggtttga ttatcgaacc cgtttcaaag ctcaaaaacg gcgagcgcat cgcgcattat
     1321 gaaatattgc ggcaaatcgg cgaaggcgga atgggcgaag tttatctggc gcgagatacg
     1381 cgcctgaatc gccgaatcgc gctgaaggct ctgcccgccg acctgaccgc cgacccgctg
     1441 cgtcttcagc gctttaagca ggaagcgcag acggcttccg cgctcaatca cccgaacatc
     1501 atcaccattt acgaaatcgg cgacgccgcg ggcgttcatt ttatcgccac cgaattcgtc
     1561 gaaggcgaaa cgctgcggca aaaaatggcg aaaaacgatt taagtatagc ggacacgctc
     1621 gatattgccg cgcagacggc ttcggcttta gccgtcgctc attcgacggg catcattcac
     1681 cgcgacatca agcccgaaaa tataatgatt cgccccgacg gaatcgtaaa gctgctcgat
     1741 ttcggtctgg cgaagctgac cgaaggcggc gcgagaacaa acgccgaaaa ttttctgacc
     1801 aatcccggaa tgattatggg aacggtcaat tatatgtcgc ccgaacaggc gcgcggcgcg
     1861 gcgatcgatt atcgcaccga cctttggagt ctgggcgtcg tgctttacga aatgctttcc
     1921 ggcgttttac cgtttcaagg caaaacggtc aatcacacat tagtggcgat tatggaagcc
     1981 aatccgtcgc tgcgctcgca gagcaatcgt gaaataccgg acgagctcga acaaatcgtc
     2041 gccgtgcttt tgtcgaaaga gccgcacaaa cgctacggtg cggcgaatga attgctggcg
     2101 gatttgcgcg cgctgcggaa attaaccgaa tcgcagccgg aaaaaaccgg aatccggcat
     2161 ttcgccgcgc cttcgacgcg gcagccgctt tccgaaaaag aatccgtcgc ggaaatcggt
     2221 gctccggcga tcgctccgaa taacttgtcc ggtcagcttt tgccgctcgt cggtcgcgaa
     2281 aaggagctct ccgaaataat aaatctgctc gggcgcgaag acatccggct tttaactttg
     2341 accggcgtcg gcggaacggg aaaaacccgg ctcgcgcagg aaatcgccgc cgcgatgctc
     2401 gcggaatttg cggacggcgt gttttttatc gctctgcccg ccgttagaaa tcccgagttc
     2461 gttatctcgg aaatcgccca gccgctcggc gtcagggatg cgggcggcaa atctctgctc
     2521 gatacgctga aagatttttt gaaagccaga cgaatcctgc tcgtcgtcga taatttcgag
     2581 caggtcctgc ccgccgcgcc tctgttaaac gaattgcttg ccgccgcgcc gcgctcaaaa
     2641 atgctggtca cgagccgcgc gctgctgcgc ctgcgcgccg agcgtgaatt cgtcgttccg
     2701 ccgcttgacg tgccttcgga catcgagcga actgcggcgg ctgatttact tcaatacgaa
     2761 tcggtcaggc tttttacgaa acgcgcgcag gcggcgaaac cggctctggc gctgaacgaa
     2821 gaaaactcga aaattatcgc cgaaatctgt tcgcggctcg acggtttgcc gctggcaatc
     2881 gagcttgccg ccgcgcgcgt caagattctt tcgccgcagg cgattttgac cagactggaa
     2941 aacagtctga gcctgctgac cggcggcggg cgggatttgc tcgaacatca acaaacaatg
     3001 cggcgcgcca tcgaatggag ttatgatttg ctcgacgagc gggaaaagat tttgttcaga
     3061 cgactggcgg tctttgcggg cggtttcaca atcgatgcgg cggaagcagt ctgcagccgg
     3121 caaaacgccg aaaacggcac agagcattct ttatccggcg gcgagcgaat atcgccggca
     3181 gccaatcgcc tgttgacgac cgagattctc gaccttatta catcgttaat cgaaaacagc
     3241 ctgctcgtcc aaaaggaaac ggcagacgat gaatcgcgtc tgcgaatgct ggaagtagtg
     3301 cgcgaattcg cgctcgaaaa attggaagcg agcggcgaaa gccttgcggc gaaaaaagct
     3361 cacgccgcgt tttttctcgc gctggcgcaa aaagccgagc ccgagatttt cggcgagcag
     3421 ggaggaaaat ggctcgaccg gctcgaagaa gaacacgaaa atctgcgcgc cgcgctttcg
     3481 cgctcgactg ccgccggcag cgtaacggct gccgagctgg ctggcgcgtt gcggaatttc
     3541 tggattctgc acaatcacat aaccgaggga cgcaaatggc tggagacgat attggagcaa
     3601 agcgccgaag cgccgctcgc cgtgcgcttg aaattaatca acgggctcgg acacgcggcg
     3661 ggctaccagg gcgatttgga gatggcgcga aaggcgcacg aaaaaggttt ggcggaaggc
     3721 aaagccgcca acgatttgcg tcaaatcgcg ctgtcggtca gaggcttggg ttttgtcgcg
     3781 aaatggaaag acgacatacg gacggcgaga aaattttatg aagaaggctt ggcaatcagc
     3841 cgccgtctgg aagaccggaa cggaatcgcc gtctcgctca cggctctggg cgatttggca
     3901 cgaatggcag acgattatgc gacggcgcgc ccgttgtttg aggaatctct ggagatttgc
     3961 aaacagaccg gcaataaaca aggcgtggtc ggcagcttga acaatctcgg agcgattact
     4021 ttcggcgaag gcgattacgc ggcggcgcac tcatattacg cggaagcaat gaaagcggtg
     4081 ctggaattcg gcgaaaaaat ctctttatca tacgcgctcg acgggctcgc cgctctcgcc
     4141 gtccaaagcg gcgatttcga gcgcgcggcg cagcttgcgg gcatagcgga acatctgcgc
     4201 gaatcgctcg gttttgaaac cgaacccgcc gagcgccgtt ttcgcgacgc ttatctcgtt
     4261 aagctgcaaa gcgttctgga caaagaaatt ttcaccgttt tttatgaaca gggacaagct
     4321 ttaccgttaa aaaaagccgt cgctctcgcg ctgaatcaat caaataccga aacgggcgac
     4381 ccggtaaaat ctttttcggc tccgggcaaa aatccaaatc ccgtaacatc aatcgccgtt
     4441 cttccgtttc tggctttggg ttttaccgaa gacgaagaat atctgggact cggtctggct
     4501 gacgcgctta ttacgcagct cagccagacg cggcaattat ccgtgcgccc gaccagcgcc
     4561 gtgcgcagtt atacggacgg acggcgcgag gcggcggcaa tcggaaaaaa attaaatgtc
     4621 ggcgcggttt tggaaggcaa cctgcaaaaa ttgggcgagc ggctgcgcgt caccgttcag
     4681 ctgactaaca tcgaatcgga cgcgacgctc tgggcggaca aatacaacgt caatttcacg
     4741 gatttttttg aagtgcagga ccagattgcc gaacaggttt caaaggcgct gctgcttcag
     4801 ctcagcagtt ccgaacagac gcagatcaac aagcgattca ccgaaaacaa cgcggcttat
     4861 ctggaatacc tgaaaggtcg tcattttttg gaaaaacgcg acgtcgacgg atttcacaaa
     4921 gccatcgagc actacaaaaa ggcgattgat ttcgacccga ctcacgcgct ttcttatgtc
     4981 gggctttccg acgtttactg tatgttcgga atctggggcg aatacccgca ggaagaaatt
     5041 tttccgcgcg cgaaagcggc ggctctgcgg gcgctcgaaa tcgacgaaaa gctggctgaa
     5101 gcgcacgctt cgctcggtcg cgtcagcagc gtctacgaat ggaattggga ggcggcggaa
     5161 aaatcctacc ggcgggcgat cgagctcaac ccgaattgcc tgaacgcgca cagctggctg
     5221 gcgaaagttt atgttctcag acaccaatac gacgaagcga ttgaagaaag ccgcatcgcg
     5281 cagcggctcg acccgctttc gccgcaggcg gaagtttcgc tttgcgcggc ttatttttac
     5341 tcgcgccggt acacggaagc cgtcgaaaaa tacagaaaag cgatgcggat gcatcctgat
     5401 ttcgttccgg cgattttcgg actcggaatg tgtctggcgg aaaaaggcga atttgcagag
     5461 gcaatcgccc tgcaggaaaa agccgtcgag ctttccaaaa atcatccgct catcgaagga
     5521 tttttagccg gaacctatgc ctactcggga aatattaaaa aggctctgga gtcgatagaa
     5581 aaattcaaag ccgccgaaga caaatctctg tattacacta tcgccgccgt ctatgccaga
     5641 ttgaacgaaa aggaggaagc ttttgcatac ctcgaaaaat ctttctcgat gcgcgtccgc
     5701 agcctgtcgg gcatcagcat cgaaccggaa ttcgacaacc tgcgcggcga accgcgcttt
     5761 cgggatatgc tgcgccgcgt cggtttagat gtgtgaaagg cggataaagc aatgttcctt
     5821 tatccgcctt tcgctcattc acaaatcctt tttaacttct aagataatgc cgtataaata
     5881 aaccaaacaa aaaactaaag cgtcagcccg attgttttga gagaatcccg tcggcgcgcc
     5941 caacaaaaat tttgcgcgcg accgaaagcg gcaaatcaca aatttgtcgg ttcgtccgaa
     6001 tgcggcacga agcatttgcg gcagcgcgcc tcgtatttat ccgccgcgcc gacttcgacg
     6061 acggcgtccg attcgacggt tctttgggaa taattggcgg tttcgccgca tttgacgcag
     6121 atggcgtgag ttttcgtgat aaattcggcg accgccagca gctgcggcat cggctcgaac
     6181 ggtctgcccg tgtaatcctg gtcaagcccg gcgacaatca cgcgcattcc gcgattcgcc
     6241 agaaaattta cggcgttgac cacttccaaa tcgaaaaact gtccctcgtc aatcccgacg
     6301 acttccgtat ccacatcgat tttatcgagc atttccttag ctgacgaaac cggaatcgag
     6361 ccgtgtttga cgcccgaatg cgaggcgatt tcctcgaccg aataacgaac gtcaatcgcc
     6421 ggtttgaaga cctgcacttt ctgccgcgcg atgttggcgc gtttcaggcg gcgaatcaat
     6481 tcctcagatt ttcccgaaaa catcgagccg acgatgactt ccacccagcc gacgccgttt
     6541 ttgcgaaatt tgcggcgctc ttccgtattg tcgaaagcga attcttcaac cataaatgcg
     6601 ttataaattc ctcttttgtt tgttatttga aaggaaaaaa agattaccac gaaagccg
//
LOCUS       DASWNE010000992         1955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_702750, whole genome shotgun sequence.
ACCESSION   DASWNE010000992 DASWNE010000000
VERSION     DASWNE010000992.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702750"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VGB68_19155"
     CDS             <1..658
                     /locus_tag="VGB68_19155"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HEY0051423.1"
                     /translation="AVIRAILLDPEARGNIKTDPDYGHLREPVLYVTNFLRPFNPAAQ
                     ANPVAAENCAGLSDGMINNVTLTLDQDVYNPPSVFNYYPMDYVIPNTPLAGPEFGIFS
                     TGTALKRPNFVHQMIGPGAGGATGITVVGNAAAGNYTPCGTRIDLVRLQSLATSDTTG
                     GALVDTLNREMMHGAMSPQMRSYIMTAVQAVTASDGGLKRARTAVYLVATSPQFQVQR
                     "
     gene            827..>1955
                     /locus_tag="VGB68_19160"
     CDS             827..>1955
                     /locus_tag="VGB68_19160"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEY0051424.1"
                     /translation="MAAIATQMRHFGMVSALAQKSPDASPEGGDYKALVCVFLSGGND
                     SNNMVVPKHTEGYNQYAAARQAQGLAIPQASLLSIMPPAMGGLEFGLHPSLSGLHTLW
                     GQGKLAVVTNVGTLVQPITRAQYQAGAPRPMQLFSHSDQVEQFRTAISSYRSTTGWGG
                     RVADRTGALNPGAAIPTVTSIAGATVFNIGNNTSPLIVAAAPTPLNQVLTLNGFGTAA
                     DETARRTAMDNIRTADLNYSMIQATSVLTQQAVNVSQQLSQNPTLTVTFPTTTLGNQL
                     AQVAKLMKFRTQLNMSRQIFYVNVGSFDTHSGQLNGQGNLLTQVSQAMKAFYDETVAQ
                     GIAADVTTFTMSDFNRTFNPAGSGSNVGTDHAWGGHHLVMGG"
BASE COUNT          432 a          559 c          575 g          389 t
ORIGIN      
        1 agccgttatc cgggcgattc tgctcgaccc ggaagcgcgc ggcaacatta aaaccgaccc
       61 cgattacggg catttgcgcg agccggtttt gtatgtcact aatttcctgc gcccgtttaa
      121 tccggcggcg caagccaatc cggtggctgc cgaaaactgc gccgggctga gcgacggaat
      181 gataaacaac gtaactttga cgcttgacca ggacgtttac aatccgccga gcgtattcaa
      241 ttattacccg atggattacg tcattccgaa cacgccttta gccggaccgg agtttggaat
      301 tttctcgacc ggaacggctt tgaaacgtcc gaacttcgtt catcagatga ttggtcccgg
      361 cgcgggcggc gcgaccggca ttaccgtcgt cggcaatgcc gccgccggta attacacgcc
      421 ctgcggaacg cggatcgacc tggtgcgatt acagtcgctg gcgacttcgg atacgaccgg
      481 aggcgctctg gtcgacacgc tcaaccggga aatgatgcac ggcgcgatgt caccgcagat
      541 gcgcagttat attatgactg ccgtgcaagc cgtgaccgca tcggacggag ggctgaaacg
      601 cgcccgcacc gccgtttatt tagtggcgac ttcgccgcaa tttcaagtgc aaagatagtt
      661 attggttctt cgtccgctgt gcgtcgtccg ttgttagtcg aatgaaatcc gagaattcag
      721 atttcattcg accggcacag gacaaagggc aaaggacgaa ggacaaatta ggagaattat
      781 gaaaagtaga agggaattct taaaaacatc ttgtcgcgct ttgagcatgg cggcaatcgc
      841 tacgcagatg cggcatttcg ggatggtcag cgcgctggcg caaaagagtc cggacgcgag
      901 cccggaaggc ggcgactata aagctctggt ctgcgttttt ctgtcgggcg gcaacgacag
      961 caacaatatg gtcgtcccga aacatacgga aggctacaac caatacgccg cggcgcggca
     1021 agcgcagggt ttagcgatac cgcaagccag cctgctgtcg attatgccgc cggcgatggg
     1081 cggcttggaa tttggtctgc acccgtcgct gagcggtttg cacacgcttt gggggcaggg
     1141 aaaactcgcg gtcgtgacta atgtcggaac gctcgtccag ccgataaccc gtgcgcagta
     1201 ccaggcgggc gcgccgcgtc cgatgcagct tttttcgcac tccgaccagg tcgagcagtt
     1261 tcgcacggcg atttcgagtt atcgctcgac gaccggctgg ggcggtcgcg tcgccgaccg
     1321 caccggcgcg ttaaatcccg gcgccgcgat tccgaccgtg acttcgattg ccggagcgac
     1381 ggttttcaac atcggcaaca acacctcgcc gctgatcgtc gccgccgcgc cgacgccgct
     1441 taatcaggtt ttgacactga acggcttcgg cactgccgcc gacgaaaccg cgcgcagaac
     1501 ggcaatggat aatattcgca cggcggattt aaattattcg atgatacagg cgacgagcgt
     1561 tttgacgcag caagccgtca atgtcagcca gcagctcagc cagaatccga cgctgaccgt
     1621 gacttttccg accacgacgc tcggcaacca gctggcgcag gtggcgaaat tgatgaagtt
     1681 ccgcacgcag ctgaatatga gccgacagat tttctacgtc aatgtcggca gtttcgatac
     1741 gcacagcgga caattgaacg gacagggcaa tctgctgacg caggtcagcc aggcgatgaa
     1801 agcgttttac gacgaaacgg tcgcgcaggg catcgccgcc gatgtcacca ctttcacgat
     1861 gtccgatttc aaccgcacgt tcaatccggc gggcagcggc tcaaacgtcg gcaccgacca
     1921 cgcctggggc ggacatcatc tggtgatggg cggct
//
LOCUS       DASWNE010000993         3285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_628731, whole genome shotgun sequence.
ACCESSION   DASWNE010000993 DASWNE010000000
VERSION     DASWNE010000993.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628731"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VGB68_19165"
     CDS             <1..277
                     /locus_tag="VGB68_19165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051425.1"
                     /translation="AGPGRVAPWRASLPVELDEFVEKIPFKETKGYVQGVIRNSAQYR
                     RLYDESGNFKPNVGTKPIRNVLDTQTRERIAEELPEVKIDESRQAEE"
     gene            complement(284..985)
                     /locus_tag="VGB68_19170"
     CDS             complement(284..985)
                     /locus_tag="VGB68_19170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051426.1"
                     /translation="MKTAGLNQLRKAATIIVVGLFVFASAIKTDAQQTVDKTVATISD
                     GVSSSELITYSDLLWQLALNPDAPLNPPNSDDLKKILQILIDQRLFALEAKRLPRNAP
                     TEAEVNEEIKRVLALFPSTAEFERRLRSVGFESVTDQNFVRMMEQRVAIVKYVDFRFR
                     SFVIITPQEEEKYYRETFTPDFRRRNPGVLVPALAEARPFVNQILTERKVEEDIERFL
                     DETKRRVEIEILFEV"
     gene            1169..2824
                     /locus_tag="VGB68_19175"
     CDS             1169..2824
                     /locus_tag="VGB68_19175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0051427.1"
                     /translation="MFSPKLAKKKTEAFAISQTVADSALELKFIAELGRSLLFTFHPK
                     KVASRVTEALRREVGAEVCALVVELESVGFVSSAFDSKDCETNDFLHRSRFKKWLKFL
                     PPQISVWAEDEKEFLLSGKTHKFEYVSPLHINGQVKGAVVVGFAEKHFCDERAQRLID
                     AATQMAAMSVNLSAHYEATMDASINQAKEEHRKFTETVLDALPVSLYVIDKDYRIVTW
                     NKNREIGLQGVPREEVIGRNVFEVLTKYPQSELSQEFERAFRTGKIERIEQRTTDPNG
                     ASKHWLVSKIPMRDSDTGEVTHVITVGEDVTVRVEAIHAVGRAEKLAAVGRLASGVVH
                     EINNPLATISACAEALETRVTEGVFDVSPEVEDLREYLSLIRSEAFRCKTITNGLLDF
                     SRVRTGNRTPIDMGLTIKSSARLIAHQKRGDNIEINLELEENLPPINADEGQMQQAVI
                     ALSTNAIDAMAGGGLLTLRASSQNGRVVVEVQDTGHGIAPEDLPKVFEPFFTTKEIGK
                     GTGLGLAVCYGIVTEHGGRLAVRSNVGVGTTFTMYLPIHKEQS"
     gene            2914..>3285
                     /locus_tag="VGB68_19180"
     CDS             2914..>3285
                     /locus_tag="VGB68_19180"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY0051428.1"
                     /translation="MAKILVIDDESNLRLVVQKEMSRLGHEVETASDGEAAWTVLEEK
                     DYDVLLCDINMPRLDGIGLLRRLKEKRQNPPEVIMLTGHATVETAIEAMKLGAYDYLT
                     KPYRITELAVLVTQAAEKQKLK"
BASE COUNT          845 a          832 c          895 g          713 t
ORIGIN      
        1 cgccggaccc ggacgagttg cgccgtggcg cgcgagcctg ccggttgaac tggacgagtt
       61 tgtcgaaaaa attccgttta aggaaacgaa aggctacgtg cagggcgtga ttcgaaattc
      121 ggcgcagtat cgccgccttt acgacgaaag cggaaacttc aagccgaacg tcggcacgaa
      181 accgattaga aacgttctcg atacgcagac gcgcgaaaga atcgccgaag aactgccgga
      241 agttaaaatc gacgaaagcc ggcaggcgga agagtaagaa aatttaaacc tcaaacaaaa
      301 tttcgatttc gacgcggcgc ttcgtctcgt ccagaaatct ttcgatatct tcttcgacct
      361 ttcgctcggt cagaatttga ttaacgaacg gacgggcttc tgccagcgcc ggcacgagca
      421 cgcccggatt gcggcggcgg aaatcgggcg taaaagtttc gcgataatat ttttcttctt
      481 cctgcggcgt gataatcaca aacgagcgaa agcgaaagtc cacgtatttg acgatggcga
      541 cgcgctgttc catcatacgg acgaaattct ggtcggtgac cgattcaaag ccgaccgagc
      601 gcagacgcct ttcaaattcg gcggtcgaag gaaaaagcgc cagaactctt ttgatttcct
      661 cgtttacttc cgcttcggtc ggcgcgttac gcggcagacg cttggcttcg agcgcgaaaa
      721 ggcgctggtc aataagaatc tgcaatattt ttttgaggtc gtcggaattc ggcggattaa
      781 gcggcgcgtc cggattgaga gcgagctgcc agagcaaatc ggaataagtt atcaattcgg
      841 aactggaaac gccgtcgctg atggtggcga cggttttatc gaccgtttgc tgcgcgtccg
      901 tttttatcgc cgaagcgaag acgaacaatc cgacgacgat aatcgtcgcc gctttccgca
      961 actgatttaa acccgcagtt ttcataagct tgacattaag attcttgttt aaataaaaaa
     1021 aagagtcaaa ttattgtttc cgtttaaaat aaaaaacttg acaaagtgtt aaaaatcttt
     1081 ttcaatcgtt aaaggaagtt ttaaacttct ctttcgtcca atttccctca gatgccaaat
     1141 caatagataa gtttgtcgcc cagaaataat gttttctcca aaattagcca aaaagaagac
     1201 ggaggcgttt gctatcagtc aaaccgtagc ggattctgct ctggaattaa aattcatcgc
     1261 cgagctcggt cgcagtctgc tgtttacctt tcatccgaaa aaggtcgcca gccgcgtcac
     1321 cgaagcgctg cgcagggaag tcggggcgga agtctgcgcg ctggtcgtcg agctggaaag
     1381 cgtcggtttt gtcagttcgg cttttgattc caaagattgc gaaacaaacg attttctgca
     1441 ccggagccgg tttaaaaagt ggctgaaatt tctgccgccg cagatttccg tgtgggcgga
     1501 agacgaaaag gaatttttgc tgagcggaaa aactcataaa ttcgaatacg tttcgccgct
     1561 gcacatcaac ggacaggtca aaggcgcggt agtggtcggt tttgccgaaa aacatttttg
     1621 cgacgagcgg gcgcagcgtt tgattgacgc ggcgacgcag atggcggcga tgtcggttaa
     1681 tctttccgcg cattacgaag cgacgatgga cgcttcgata aatcaggcga aggaagaaca
     1741 ccggaagttt accgaaaccg tgctggacgc tttgcccgtt tcgctctacg tgattgacaa
     1801 agattatcga attgtcactt ggaacaagaa ccgcgaaatc ggtttgcagg gcgttccgcg
     1861 cgaggaagtc atcgggcgca acgttttcga agttttgacg aaatacccgc agagcgagct
     1921 cagccaggaa tttgaaagag cgtttcggac aggcaaaatc gagcgcatcg aacagcgcac
     1981 gaccgacccg aacggcgcga gcaaacactg gctggtcagc aaaatcccga tgcgcgattc
     2041 ggacacgggc gaagtgacgc acgtcatcac ggtcggcgag gacgtgacgg tccgcgtcga
     2101 agcgattcac gccgtcggtc gcgccgaaaa gctggcggcg gtcggcagac tagcctccgg
     2161 cgtggttcac gaaatcaaca acccgctcgc caccatcagc gcttgcgccg aagctctgga
     2221 gacgcgcgtg acggaaggcg ttttcgacgt ctcgcccgaa gtcgaagatt tgcgcgaata
     2281 tctgagctta atccgcagcg aagcgtttcg atgtaaaact attaccaacg gcttgctgga
     2341 tttcagccgc gtgcggacgg gaaaccgaac gccgattgat atgggcttga ccatcaaatc
     2401 ttccgcccgt ctcatcgctc accagaaacg cggcgacaac atcgaaatca acctcgaact
     2461 ggaagaaaat ctgccgccca tcaacgccga cgaaggacag atgcagcaag ccgtcatcgc
     2521 cctttcgacc aacgcgattg acgcgatggc gggcggcggt ctgctgaccc tgcgcgcttc
     2581 ttcacaaaac ggtcgcgtcg tcgtcgaagt tcaggacacc ggacacggca tcgcgccgga
     2641 agatttaccg aaagttttcg agccgttttt taccactaag gaaatcggca aaggcacggg
     2701 tctgggtttg gcggtttgct acggaattgt caccgaacac ggcggtcggc tggcggttcg
     2761 ctcgaatgtc ggcgtcggca cgacgtttac gatgtatctt cccattcata aagaacagtc
     2821 ctgagccctg agccctgagt cctgagcttc tttgtctcag gactcagcac ttagaactca
     2881 tcacttgaaa ctcagcactc tcaaaataaa tctatggcaa aaattttagt tatcgacgat
     2941 gaaagcaatc tgcggctggt ggtgcagaag gaaatgagcc gtctcggtca cgaggtcgaa
     3001 accgcttcgg acggcgaagc cgcctggacg gtgctcgaag aaaaagatta cgacgttctt
     3061 ctgtgcgaca tcaatatgcc gcgtctggac ggcatcgggc ttttgcgtcg tttgaaagag
     3121 aaacggcaaa atccgccgga agtcattatg ctgaccggac acgcgacggt cgagaccgca
     3181 atcgaagcga tgaagctcgg cgcgtatgat tatctgacca agccgtatcg catcaccgag
     3241 cttgccgtgc tggtgacgca agccgccgaa aaacagaagc tgaaa
//
LOCUS       DASWNE010000994         1654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_564352, whole genome shotgun sequence.
ACCESSION   DASWNE010000994 DASWNE010000000
VERSION     DASWNE010000994.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564352"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VGB68_19185"
     CDS             complement(<1..515)
                     /locus_tag="VGB68_19185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEY0051429.1"
                     /translation="MSKVIGIDLGTTNCCVSVLEGGTVQIIPNKEGGRTTPSVVGFTE
                     KGERLVGQIAKRQAVTNPANTLYAVKRLIGRKYNSAEVEKMRETVPFDITQAPNSDAH
                     IRVLDRVYSPPEISAIVLQRLKLAAEEFLGDKISEAIVTVPAYFDDAQRQATRDAGKI
                     AGLNVERIINEP"
     gene            complement(581..1408)
                     /locus_tag="VGB68_19190"
     CDS             complement(581..1408)
                     /locus_tag="VGB68_19190"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEY0051430.1"
                     /translation="MNSNNDSPEQFIENIEADNLTSLDEFIKELEAKEKDLNITSEIF
                     VEIEEYDEFENSNQPGIVDLGKIYETRNLQESSASAAPPAAEAQQLNKELAELRQQVR
                     RLEKEREDMREVLSRRQNDFENYRKRIERDRQETFRTLVGDLATQMLPVVDNLERALD
                     SSDALADGKSPEFQHFLDGIFLVNQQLGEVLAEMGIEPIYSVGKTFDPHFHEAAATEE
                     TAEYPPNTVTKELLRGYRMDEKVIRAAIVKVSAAGSTGNRPSPPAFQEIDLNSFKNE"
     gene            complement(1444..>1654)
                     /locus_tag="VGB68_19195"
     CDS             complement(1444..>1654)
                     /locus_tag="VGB68_19195"
                     /inference="COORDINATES: protein motif:HMM:NF013768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HrcA family transcriptional regulator"
                     /protein_id="HEY0051431.1"
                     /translation="QIIIGSENSAPSLQNCTLITAPYRLGNDSAFGTLSVLGPTRIEY
                     ARMISIVSYVARMLEKMMIDDKSNS"
BASE COUNT          328 a          425 c          455 g          446 t
ORIGIN      
        1 ggctcgttga tgatgcgctc gacgttcaga ccggcgattt ttccggcgtc gcgcgtcgcc
       61 tgccgctgcg cgtcgtcaaa atacgcgggc acggtgacga tggcttcgct gattttatcg
      121 cccagaaatt cttccgccgc cagcttcaga cgctggagaa cgatcgccga aatttccggc
      181 gggctgtaaa cgcggtcgag cacgcggata tgcgcgtccg agttcggagc ctgcgtgatg
      241 tcgaacggga cggtttcgcg cattttctcg acttccgccg agttgtattt gcgaccgatt
      301 aaacgtttga cggcgtaaag cgtgtttgcc ggattcgtga cggcttggcg cttggcgatt
      361 tgtccgacca ggcgctcgcc cttttccgta aaaccgacga cggacggcgt ggttcgtccg
      421 ccttccttgt tcgggataat ctgcaccgtg ccgccttcga gaacggagac gcagcagttg
      481 gttgttccta aatctattcc gatgacctta ctcataattt tttttgagtc ctttaattga
      541 ccgagaagaa tcgaagcgga gcagcagcag cagcgggcgg ctactcgttt ttaaaagaat
      601 ttaaatctat ttcctgaaat gcgggcgggc ttggacgatt gccggtgctg ccggcggcgg
      661 aaactttgac tatcgcggcg cgaatgactt tttcgtccat ccggtagccg cgcagaagct
      721 ctttggtgac cgtgttcggc gggtattcgg cggtttcttc cgtcgccgcg gcttcgtgaa
      781 aatgcgggtc gaacgtcttg ccgaccgaat aaatcggctc gatgcccatt tccgccagaa
      841 cctcgccgag ctgttgattg accagaaaga ttccatcgag aaaatgctgg aattcgggcg
      901 attttccgtc cgccagcgcg tcggacgaat ccagcgcgcg ctccagatta tcgacgaccg
      961 gcagcatctg cgtcgccaaa tcgccgacca gcgtgcgaaa agtttcctgc cggtcgcgct
     1021 cgatgcgctt gcggtagttt tcaaaatcgt tctggcgacg gctcaaaacc tcgcgcatat
     1081 cctcgcgctc cttttccagt cggcgaacct gctgtcgaag ctcagccaat tccttattta
     1141 attgctgagc ttcggcggca ggaggcgcgg cgctggcgga gctttcctgc agattgcgtg
     1201 tttcgtaaat ttttcccaaa tcgacaatgc ccggctgatt tgagttttca aactcgtcgt
     1261 attcttcaat ctcgacaaaa atttcggagg tgatgttgag gtctttctcc ttggcttcga
     1321 gttccttgat aaactcgtcg agcgaagtca gattgtcagc ttcgatattt tcgataaatt
     1381 gttctggtga atcgttattg gaattcattt tgggtttgta aaattatttt cgtttggaaa
     1441 aaatcaggag tttgatttgt cgtctatcat cattttttcg agcattcgcg ccacgtagct
     1501 gacaatggaa atcattcggg cgtattcgat tctggtcggt cccaaaacgc tcaaagtgcc
     1561 gaaagccgaa tcatttccga gacgatacgg cgcggtgata agcgtgcagt tttgcagcga
     1621 gggcgcggag ttttcgcttc cgattataat ctgg
//
LOCUS       DASWNE010000995         2593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_172600, whole genome shotgun sequence.
ACCESSION   DASWNE010000995 DASWNE010000000
VERSION     DASWNE010000995.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172600"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VGB68_19200"
     CDS             complement(<1..418)
                     /locus_tag="VGB68_19200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY0051432.1"
                     /translation="MQSEKKHTNHLINETSPYLLQHAHNPVEWYSWGEEAFEKARAED
                     KPILVSIGYSACHWCHVMEHESFEDEQIAAIMNEHFVNIKVDMEERPDVDQIYMSFVQ
                     MTTGSGGWPMNVFITPDKIPFFGGTYFPPAPRYGMPS"
     gene            850..2205
                     /locus_tag="VGB68_19205"
     CDS             850..2205
                     /locus_tag="VGB68_19205"
                     /inference="COORDINATES: protein motif:HMM:NF004426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HEY0051433.1"
                     /translation="MTPTATELQKPIIKTELPGPKGREIVEADRQYVTPSYPRPDYKL
                     VAEKGYGVWIEDVDGNVFLDCNAGVAVCSTGHCHPEIVKAITEQASTLIHMCGTDFYY
                     RHMPALAQKLNEICPIEGETKTHFANSGAEAVETALKVAMHHTRRQKFISFFGSFHGR
                     TLGALSLTSSKAAQRRGFMRQALDVVHVPFPNKFRHFANNLPTDEETVTRDCISWIEE
                     RIFKTTTPPDEVAAIVVEAVQGEGGYVPAPYGFLQAIRRICDEHGIVMIVDEVQSGMG
                     RTGKMFAIEHSGVSPDVMCLAKGIGSGLPIGVCVAKDSIMNWGKGAHASTFGGNPVCI
                     AAALKTIELLENGLTENSANVGEYLQAGLRKLQDKYDCIGDVRGLGLMLGVEFVTDKE
                     TLKPAPELRDKIEMECFNRGLIILGCGASTIRWSPPLILTKENSDVALDIFDEAISAS
                     I"
     gene            complement(2400..>2593)
                     /locus_tag="VGB68_19210"
     CDS             complement(2400..>2593)
                     /locus_tag="VGB68_19210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051434.1"
                     /translation="RERRSTPLISSLYTLGYASDCCSVAVQAFTYNVGVRRENRYVFS
                     FRLKGIGTFGTEQFGQGLR"
BASE COUNT          630 a          660 c          692 g          611 t
ORIGIN      
        1 agctcggcat gccgtaacgc ggcgcgggcg gaaaatacgt gccgccgaaa aacggaattt
       61 tatcgggcgt gataaaaaca ttcataggcc agcctcccga cccggtcgtc atctgcacaa
      121 agctcatata aatctggtcg acatcgggtc gctcttccat atcgactttg atgttgacga
      181 aatgctcgtt cataatcgcg gcgatctgct cgtcttcaaa cgattcgtgt tccatgacgt
      241 ggcaccagtg acacgccgaa taaccgatgc tgaccagaat cggcttgtct tcggcacggg
      301 ctttttcaaa tgcttcttcg ccccacgaat accattcgac cggattatgc gcgtgctgga
      361 gcagatacgg actcgtttca ttgattaaat gattggtgtg ttttttctcg ctttgcatag
      421 ctttattgtt tcacgccgta atggcgcgac gcaaagttta tgaaggcaaa agtagaatgc
      481 aaaagggtaa aattaagagc cgggaaacag cggcgcgaga aatattcagt ttcagatttc
      541 atccggcgtt ttttcagctt tactttttac tttcggcttt ttgcttttgc cttcaaagac
      601 ttttgtccgg aaatctttga atccgcggac gcattttgat atattgtttc cgtaattctt
      661 acctttatga cgccgcgctc gatagccaaa ttaagacctg tcataacgcg cccgaatctt
      721 ttcgccggcg actagatttt tctccccgac gtttcagcgt ttagcgtcgg ttttatccgg
      781 cattgccaaa tcacgcggcg aaatttttcg ccggctaaat catttcaata aaattttgag
      841 gagaaattta tgaccccgac cgctaccgaa ttacaaaaac cgattatcaa aaccgagctt
      901 cccggaccga aaggcagaga aatcgtcgaa gccgaccggc agtacgtcac gccctcgtat
      961 ccgcgccccg actataagct cgtcgccgaa aagggttacg gcgtctggat tgaagacgtc
     1021 gacggaaatg tctttctgga ctgcaacgcg ggcgtcgcgg tttgctcgac gggacattgc
     1081 cacccggaaa tcgtcaaggc aatcaccgaa caggcttcga cgctgattca tatgtgcggc
     1141 acggattttt attaccggca tatgcccgcg ctggcgcaga aattaaacga gatttgcccg
     1201 attgaaggcg aaacgaaaac gcatttcgcc aattccggcg cggaggcggt cgaaaccgct
     1261 ctgaaagtgg cgatgcatca cacgcgccgc cagaaattta tctcgttctt cggctcgttt
     1321 cacggcagaa cgctcggcgc tctgagcctg acctcttcca aagcggcgca aagacgcgga
     1381 tttatgcgac aggctttaga tgtcgttcac gttcctttcc ccaataaatt ccggcatttc
     1441 gccaacaatc tgccgaccga cgaggaaacc gtcacgcgcg actgcatcag ttggattgaa
     1501 gaacgcattt tcaaaaccac gacgccgccc gacgaagtgg cggcaatcgt cgtcgaagcc
     1561 gtgcagggcg agggcggcta cgtacccgcg ccgtacggat ttttgcaggc gatcaggcgc
     1621 atctgcgacg agcacggcat cgtgatgatt gtcgacgaag tgcagtcggg aatgggcaga
     1681 accggaaaaa tgtttgccat cgagcattcc ggcgtctcgc cggacgtgat gtgtctggcg
     1741 aaaggcatcg gttcgggctt gccgattggc gtctgcgtgg cgaaagacag cattatgaac
     1801 tggggcaagg gagcgcacgc ttcgaccttc ggcggaaatc cggtctgcat cgcggcggct
     1861 ttgaaaacga ttgagctgct tgaaaacggt ctgacggaaa actcggcaaa cgtcggcgaa
     1921 tatttgcagg cgggcttgcg caaattgcag gacaaatacg attgtatcgg cgacgttcgc
     1981 ggtttgggct taatgctcgg cgtcgagttc gtgacggata aggaaacgct taaacccgca
     2041 ccggagctgc gcgataaaat cgagatggaa tgcttcaatc gcggtcttat aattctcggc
     2101 tgcggcgcga gcacgattcg ctggtcgccg ccgctgattt tgacgaaaga aaactcggat
     2161 gtcgcgctcg atattttcga tgaagcgata tccgcttcaa tctaaccgga cttgccgaaa
     2221 ccgaaattca tcgaggtcga gaaccggaga aaattctttc gattatagcc gaacaaataa
     2281 cgcagaaccg gaaaatgatt ttcgattcga caatctttgt tcaaaatatt gagatgcggg
     2341 ttttgggctt cagattttca tctgaagccc cgaccgacag aaaaaggaaa tatttgtact
     2401 taacgcaaac cttgcccgaa ttgctccgtg ccgaatgtgc cgatgccttt caggcggaag
     2461 ctgaaaacgt agcggttttc gcggcgcacg ccgacgttgt aggtaaaggc ttgaacggcg
     2521 accgaacagc aatccgaggc gtagccgagc gtgtagagcg agctgatgag cggcgtcgag
     2581 cggcgctcgc ggg
//
LOCUS       DASWNE010000996         9330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_244568, whole genome shotgun sequence.
ACCESSION   DASWNE010000996 DASWNE010000000
VERSION     DASWNE010000996.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9330
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244568"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VGB68_19215"
     CDS             <1..346
                     /locus_tag="VGB68_19215"
                     /inference="COORDINATES: protein motif:HMM:NF013395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEY0051435.1"
                     /translation="SYCRTLAQAGQELYIISGVEGNIGTIAQGRIVVPRNTWKVVLVL
                     PNGSDDLARVSKSTRVIAIVVPNFQPLNINAPWRQFRKSVDQVEALTGYNFFSNIPKN
                     TQEIMERRRDVQ"
     gene            482..2380
                     /locus_tag="VGB68_19220"
     CDS             482..2380
                     /locus_tag="VGB68_19220"
                     /inference="COORDINATES: protein motif:HMM:NF040322.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051436.1"
                     /translation="MKNLFSVLFLIVLVFISGVSAQKSRTISEIQGDKAASMFVNEPV
                     RVSGIVTARTRSGFFMQTPDDKADSNPNTSEGILVFTRTEPGSEATIGNLVTVTGTID
                     EFRPKNELSSLPITEIRFTKGKDIVEVESKNNPLPKAVALTVQDFTSNKIDQIEKYEG
                     MRVFVETLTAISPTGGRVQDKNATAESNGTFFAVVKGIPRPFREPGMDIYDYIMSPDK
                     DRLKKDYPKMPIFDNNPERLRIESTAQLGASAIDVNSKAEIKNLSGVLHYTYRTFTIY
                     VDVDSKHSISNAIKPVVLPQANDRQFSVAATNLENFFDDEDDPSMKEDIVTKEAFALR
                     LKKISGAIREYLQMPDIIGIIEAENLSALKRLAEKVNNDAVAAGKPNPKYEAFLVDGN
                     DGRGIDNGFLVKTSRVEVVSVKQFGKEEKYENPVRKSEFSLNDRPPLLLEARINDAKT
                     NKPFAVTIIVNHLKSFNGYNDEKDAPNVRLKKRLQAEYLAKLVGERQKANPGERILLV
                     GDFNAYQFNDGIVDVIGAIKGTPAPRDQVLNPSEDFLNPDLTDLVDMIKADQRYSYSF
                     DGNAQVLDHFIANDAMKRHLAGFGYLRINADYPETLRGDAARVERFSDHDIPVGYFTF
                     DEQPAAKN"
     gene            complement(2491..2709)
                     /locus_tag="VGB68_19225"
     CDS             complement(2491..2709)
                     /locus_tag="VGB68_19225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051437.1"
                     /translation="MERDNLMHGARTALNQSPEIRQWCEDFLKQKYRAINAAMNDEEY
                     EKYWRYHKPEIMHEGAAEAVQAYKMREK"
     gene            2867..4390
                     /locus_tag="VGB68_19230"
     CDS             2867..4390
                     /locus_tag="VGB68_19230"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0051438.1"
                     /translation="MKNFFLFSLLAILSLADINFAQKTVPTPKTAVTTKNLPTRAQDA
                     PVVPFVNRVLPNGLEVIVLADASVPIVTVELAVRNGSFTEPPELNGLSHLYEHMFFKT
                     NRSIMVFRCEQIQTYKRFDLYKQNNCDEHLKYKSETGDGTYLGEIGNLGITYNGTTRE
                     EVVNYYFTTTSPYVSTAIRFINDAVRFPVFDEGEFEREKQVVIGEIDRNESNPYFYLQ
                     RNLMEKLFYKYPTRKNALGSRESVGTATTEKMRTIQGRYYVPNNSALVITGDVKPEEI
                     FKIAEEVFGSWKKREVDPFKDFPLVEHPPLTKSDGIIVEQPVNNVLIQIGWHGPSIGK
                     DNPATYAADVFSYILTQPDSRFQRALVDTGLVVAADVSYYTQRNTGPITLTLVTTPEK
                     SKAALAAAYAELAKFTSADYYTDEELSNATTLLESRDLFEREKLSEYAHTLSFWWSST
                     GIEYFRGYHKNMRAVSREEINRYVKTYIQGKPRVGIALISSQSQQVAKLTPEDLIGK"
     gene            4395..5759
                     /locus_tag="VGB68_19235"
     CDS             4395..5759
                     /locus_tag="VGB68_19235"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0051439.1"
                     /translation="MKVVSCQLSFVSRFFLLTFIVLSFISVNLAQDAGRASLAGQAAQ
                     VREFDVNGLKVLVKRRPLSPTVAAGLFIRGGVKNVTAQNAGIENFMLSAMTEGSKAFP
                     RETLRRELARTGSSIGSSAGYDFSVISLASTRQNFDRSWDIFTDIALNPAFAQADINL
                     AKDRILTSLRDDTDDPDGFLQVLQNKVIYANHPYGNDPRGTLETVGRFSADDLRAYHQ
                     KTMQTSQLLLVIVGDLDADDLQKRITDSFGKLPKGAYREQPAGAFNFAQPTLDITTRS
                     LPTNYIQGVFDAPSLASPDFYAMRVATTLLRDRVFEEVRVKRNLSYAPSADMNALGIS
                     TGNIYVTAVDANQAISVMLNEIKTLKDEMVEEREISGVTGQFLTTYYVGQETNAAQAA
                     ELAKYELIGGGWRNSFGFLDKVKQVKPADVQRVSQKYMKNLRFIVLGNPSAINREIFL
                     QKSL"
     gene            complement(5917..7944)
                     /locus_tag="VGB68_19240"
     CDS             complement(5917..7944)
                     /locus_tag="VGB68_19240"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HEY0051440.1"
                     /translation="MRLANVLWAFVLLMPLLLFDTVFANPNAAAADDYIPEVTARVAR
                     ISFLRGDVQIKRADSDDWERAALNLPIVEGDQIATELNARIEIQFDRDSFLRLAENSY
                     LKITTLRDEGAALSLPQGTLSLRVLSFDKSRTYFEIDAPQTTVSVQKTGMYRVDAGDK
                     DSTQIRVTATDGAEARVYSENSGFTLRSGRSATVYLDGSNAGEWEMADAARYADEWDE
                     WVLERDARIAKLLRDAYYDKYYDRDMYGAEDLSDYGEWVFTRKYGWVWRPYRDATAGY
                     ADWSPYRYGQWRWVPPYGWTWVNDEPWGWATYHHGRWIYVDGEWCWTPYASYRSRRSW
                     WQPALVVVTWSGNLICWYPLPYDYDYYNYNGYYHRRRRGNYNNNNNTGGVNPTPSPTP
                     AIVIPAPLPGKGPKLPPLQTIPPGAVVTVAKEQFGNQIKGAKTAPLDVAKLVLSKDPD
                     TMIKEAPSLPVGIVRTATVRNEIIIEKPRVKSEPIARTGVVERKVGVPMDEEIRQKKV
                     FEDRKPVTRQRDLSGGEQVKSSSEGGGTEIRKTGAVERPPVRPVVRQDDNTDNQTPRN
                     ETPVRPPRDKPRDNEQPVVKPRRDENQIPVYIPPPAREEPKERPRQEPPVRNEPPRNE
                     RPREEPRREEPKPEPRREEPKPEPKREEPKSEPKPPLENKGNKNGKDGLNF"
     gene            8323..8775
                     /locus_tag="VGB68_19245"
     CDS             8323..8775
                     /locus_tag="VGB68_19245"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051441.1"
                     /translation="MVVLAFAESSAIQLVPDGTILIHIALILIMIWVLNRTFFRPINR
                     VIAAREKNKGGRFGEAEEILRQVAEKNARFENVMREARLEGYNKIETERGAALTEKQA
                     KVETVKAEVEQKVAVEKDALARQAEEARRQIAAEARVMAEKISSNILK"
     gene            8825..>9330
                     /locus_tag="VGB68_19250"
     CDS             8825..>9330
                     /locus_tag="VGB68_19250"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HEY0051442.1"
                     /translation="MFASIFSFIFIFASGGEHSAAGGGFAEWWHTHADPYLNYPGFEA
                     WKFINLAIFAAVMAYLLKKPLSNAFKTRREQIRAELIRAEQERRAAEARLIETESKLA
                     RLDVERENVLTKAEREAELERQRIAEQTEFEITKLREQAENEISRTAQQAKQELRRLS
                     AEETIRLA"
BASE COUNT         2291 a         2443 c         2407 g         2189 t
ORIGIN      
        1 aagctactgc cgcacgctgg cgcaagccgg acaggagctt tatattattt cgggcgtcga
       61 aggcaacatc ggaacgattg cgcagggcag aatcgtcgtg ccgcgaaaca cctggaaagt
      121 cgtgctcgtt ctgccgaacg gcagcgacga tttggcgcgc gtcagcaaga gcacgcgcgt
      181 gattgccatc gtcgttccga atttccagcc gctgaacatc aacgcgccgt ggcgacagtt
      241 ccgcaagagc gtcgaccagg tcgaggcttt gaccgggtat aacttctttt caaacattcc
      301 gaagaatacg caggaaatta tggaaagacg cagagacgtg cagtaaaaga cgcgggacgc
      361 cgttttttta agattgcctg aaagtattta agataaacct taaatacttt tgacacatct
      421 taataatatc ggacgtccca tttttctctt ttagcttgcc cgtaaaaaaa tattttgaat
      481 tatgaaaaat cttttttccg ttttatttct aatcgtttta gttttcattt ccggcgtctc
      541 agcgcaaaaa agccgcacaa tcagcgaaat tcagggcgat aaagccgcct cgatgtttgt
      601 caatgagccg gttcgcgtca gcggaatcgt cacggcgcgg acgcgcagcg gattttttat
      661 gcagacgccc gacgacaaag ccgacagcaa tccgaatact tccgagggaa ttctcgtttt
      721 cacgcgcacc gagccgggca gcgaagcgac catcggaaat ctggtgacgg tgaccggaac
      781 gattgatgaa tttcgcccga aaaacgaatt gtcgagcctg ccgataacgg aaatcagatt
      841 taccaaaggc aaggatattg tcgaggtcga atcgaaaaac aatcctctgc cgaaagccgt
      901 cgctttaacc gtgcaggatt tcacctcgaa caaaatcgac cagattgaaa aatacgaggg
      961 aatgcgcgtt ttcgtcgaaa cgctgacggc aatctcgccg accggcggca gagtccagga
     1021 taaaaacgcg accgccgaat cgaacggaac ttttttcgcc gtcgtcaaag gcattccgcg
     1081 cccgtttcgc gagccgggaa tggacattta cgactacata atgtcgccgg acaaggacag
     1141 gctgaaaaaa gattatccga aaatgccgat tttcgataac aatcccgaaa gattgagaat
     1201 cgaaagcacg gcgcagctcg gcgcttcggc gattgacgtc aactcgaagg cggaaatcaa
     1261 aaacctttcc ggcgttttgc attacacgta tcgcaccttc acgatttatg tcgacgtcga
     1321 cagcaaacac tcgatttcaa acgcgattaa gccggtcgtt ctgccgcagg ctaacgaccg
     1381 ccagttttcc gtcgccgcca cgaatctgga aaactttttc gacgacgagg acgacccttc
     1441 gatgaaggaa gatatcgtca cgaaagaagc ttttgcgctg cgtctgaaaa aaatctccgg
     1501 cgcgattcgc gaatatttgc agatgcccga catcatcggc atcatcgaag cggaaaatct
     1561 ttcggcgctg aagagactcg ccgaaaaagt caacaatgat gcggtcgccg ccggaaagcc
     1621 gaacccgaaa tacgaagcgt ttctcgttga cggcaacgac gggcgcggca tcgacaacgg
     1681 ctttctggtc aaaacttcgc gggtcgaagt cgtcagcgtc aagcagttcg gcaaggaaga
     1741 aaaatacgag aatccggtca gaaaaagcga attttcgctg aacgaccgac cgccgcttct
     1801 gctcgaagcc agaatcaacg acgcgaaaac gaataaaccg tttgccgtca ccatcatcgt
     1861 caaccacttg aaatccttca acggctacaa cgacgaaaaa gatgcgccga acgtgcgctt
     1921 gaaaaaacgc ctgcaagccg aatatctggc gaaactggtc ggcgaacggc aaaaggcgaa
     1981 tccgggcgag cgcattctgc tcgtcggcga ttttaacgct tatcaattca acgacggaat
     2041 cgtcgatgtc atcggcgcga taaaaggcac gcccgcgccg agagaccagg ttttaaatcc
     2101 ttcggaagat tttctaaacc cggatttaac cgatttggtc gatatgataa aagccgacca
     2161 gcgctattcc tacagctttg acggaaacgc gcaagtgctc gaccatttta tcgccaacga
     2221 cgcgatgaaa agacatctcg ccggcttcgg ctacctgcgc atcaacgccg attatcccga
     2281 aactctgcgc ggcgacgcag cccgcgtcga gcgattttcc gaccacgata tcccggtcgg
     2341 atattttact ttcgacgagc agccggcggc gaaaaattaa gataaaatca gtccgcgcaa
     2401 cacgcgaaaa agcgcgaaaa aaaagaagaa acgtttaaag agttcttctt tttttttcgc
     2461 gctttttcgc gggctgattt ttctttgctt ttacttctcc cgcattttat acgcctgcac
     2521 cgcttccgcg gcgccttcgt gcataatttc cggtttgtga tagcgccagt atttttcgta
     2581 ttcttcgtcg ttcatcgcgg cgtttatcgc gcgatatttt tgtttcagaa aatcctcgca
     2641 ccactggcgg atttcggggc tctgattgag cgccgtgcgc gccccgtgca ttaaattgtc
     2701 gcgttccata aatttacctc cgtttgtgat attgtaaaac ttattcgcaa actttgcgta
     2761 aaattaaact gttgttcgat tgaaagacac ccggaacgga atgaaatcca acctatgtct
     2821 ctcgttcggc atctgaaacc caacatcaat attggagctt ttcagcgtga aaaatttttt
     2881 tctattttcg cttctggcta tcttaagttt agccgatata aattttgccc aaaagaccgt
     2941 gccgacgccg aaaacggcgg tgacgaccaa aaatctcccg accagagcgc aggacgcgcc
     3001 ggtcgttccg tttgtcaacc gagttttgcc gaacggtctg gaagtcatcg ttctggcgga
     3061 cgccagcgtg ccgattgtga cggtcgagct ggcggtgcgt aacggctcgt ttaccgaacc
     3121 gcccgaatta aacggtctgt cgcatcttta cgagcatatg tttttcaaaa caaaccgctc
     3181 gataatggtt ttccgctgcg agcagattca aacctacaaa agattcgatt tgtataagca
     3241 aaacaattgc gacgaacacc tgaaatacaa atcggaaacc ggcgacggca cgtatctcgg
     3301 cgaaatcgga aatctcggca ttacctacaa cgggacgacg cgcgaagaag tcgtcaatta
     3361 ttatttcacg acgaccagcc cgtatgtttc gacggcgatt cgctttatca acgacgcggt
     3421 gcgttttccg gttttcgacg agggcgaatt cgagcgcgaa aaacaggtgg tcatcggcga
     3481 aatcgaccgc aacgaatcga atccgtattt ctatctgcag cggaatctga tggaaaagct
     3541 tttttacaaa tacccgacgc gcaaaaacgc gctcggctcg cgcgaatcgg tcggcacggc
     3601 gacgaccgaa aaaatgcgca ccattcaggg tcgatattat gtcccgaaca actcggctct
     3661 cgttatcacg ggcgacgtca aaccggaaga aattttcaaa atcgccgaag aagttttcgg
     3721 cagttggaaa aaacgcgagg tcgacccgtt taaggatttc ccgctggtcg agcatccgcc
     3781 tttgacaaaa agcgacggaa tcatcgtcga gcagccggta aataacgttt tgattcaaat
     3841 cggctggcac ggtccttcga tcggcaaaga caatccggcg acttacgcgg cggacgtttt
     3901 ttcatacatt ctgacgcagc cggattcgcg ctttcagcgc gcgctggtcg ataccggttt
     3961 ggtcgtcgcg gctgacgtca gctattacac gcagcgcaac acaggtccga ttactctgac
     4021 tctggtgacg acgccggaaa aatcgaaagc cgcgctcgcc gccgcttacg ccgagctcgc
     4081 caaatttacc agcgccgatt attacaccga cgaagaatta tcgaatgcga cgaccttgct
     4141 cgaatccaga gatttgttcg aacgcgaaaa gctgagcgag tatgctcaca ccttgagttt
     4201 ctggtggtcg tcgaccggaa tcgaatattt tcgcggttat cacaaaaata tgcgcgccgt
     4261 atcgcgcgag gaaattaacc gctacgtgaa aacctacatt cagggcaagc cgcgcgtcgg
     4321 cattgctttg atttcgtcac agtcgcagca ggtggcgaaa ttaacgcctg aagatttgat
     4381 tggaaaataa atttatgaaa gtggtcagtt gtcaattgtc atttgtcagt cgtttctttt
     4441 tgctgacgtt tatagtttta tcttttatct ccgttaatct ggcgcaggac gcgggacgcg
     4501 cgagcttggc tgggcaggcg gcgcaggtca gggaatttga cgtcaacggc ttgaaagttc
     4561 tggtcaagcg tcgtccttta agcccgacgg tcgccgccgg tctttttatt cgcggcggcg
     4621 tgaaaaacgt gacggcgcaa aacgcgggaa tagagaattt tatgctcagc gcgatgacgg
     4681 aaggcagcaa agcttttccg cgcgaaacgc tgcgccgcga attagccaga acgggaagct
     4741 caatcggttc gagcgccggt tatgatttca gcgtcatcag cctcgcttcg acgcgccaga
     4801 actttgaccg ctcgtgggac atttttaccg atatcgcgct gaatccggct tttgcacaag
     4861 ccgacattaa tctggcgaaa gacagaatcc tgacgagctt gcgcgacgac acggacgacc
     4921 ccgacggttt tttgcaggtt ttgcaaaaca aggtgattta cgcgaatcac ccgtatggaa
     4981 acgacccgcg cggaacgctg gaaaccgtcg ggcgcttttc cgctgacgat ttgcgagctt
     5041 atcaccagaa aacgatgcag acatcgcagc ttcttctggt aatagtcggc gatttggacg
     5101 cggatgattt gcaaaaacgc atcacggatt cgttcggaaa attgccgaaa ggcgcttaca
     5161 gggaacaacc cgccggcgct tttaatttcg cccagccgac gctcgatata actacgcgca
     5221 gtttgccgac caattatatc cagggcgttt tcgacgcgcc ttcgctcgcc agtccggatt
     5281 tctacgcgat gcgcgtggcg acgacgcttt tgcgcgaccg cgtttttgaa gaagtccgcg
     5341 tcaagcgcaa cctttcttat gcgccgagcg cggatatgaa tgcgctcggc atcagcacgg
     5401 gaaacattta cgtgacggcg gtcgatgcca atcaggcgat cagcgtgatg cttaacgaaa
     5461 tcaaaacttt aaaagacgaa atggttgagg agcgcgaaat ttccggcgtc accggacagt
     5521 ttttgacgac ttattacgtc gggcaggaaa ccaacgccgc gcaggctgcc gagcttgcca
     5581 aatacgaatt aatcggcggc ggctggcgca actcgttcgg ctttctggat aaagtcaagc
     5641 aggtgaagcc cgccgacgtt cagcgcgtct cacaaaaata tatgaagaat ctgcgcttta
     5701 tcgtcctcgg caatccgagc gcgattaacc gcgaaatctt tttgcagaaa tcgttgtagg
     5761 cgcgatagct gcaaacgcgt cctgaatata aaagaaacgg gacgccgggt tttaagattg
     5821 gataagattt attaagattt aaatcctaat ttatcctaaa caatcagaaa aacccggcgt
     5881 cccgtttctc tttaaaagac agccggaaac ctgttcctag aagttcaagc cgtctttgcc
     5941 gtttttgttt cctttattct ccaaaggagg tttcggctca gacttcggct cttcgcgttt
     6001 tggctcgggc ttcggttctt cgcgtcgcgg ctccggtttc ggctcttccc gacgcggttc
     6061 ttcgcgcggt cgctcgttgc gaggcggctc gtttctgacc ggaggctcct gccgcggacg
     6121 ctctttcggt tcttcgcgcg cgggcggcgg aatataaacc ggtatctgat tttcgtcgcg
     6181 tcgcggtttg accacaggct gttcgttatc gcgaggttta tcgcgcggcg gacgcaccgg
     6241 agtttcgttt cgcggagttt gattgtcggt gttgtcgtcc tgtctgacaa ccggacgcac
     6301 cggaggacgc tcgaccgctc cggtcttgcg gatttccgtg ccgccgcctt cagacgagct
     6361 tttgacctgc tcgccgccgc tcaaatccct ctggcgagta accggttttc tgtcttcgaa
     6421 aactttcttc tggcgaattt cttcgtccat cggaacgccg actttgcgct cgacgacgcc
     6481 ggttctggca atcggctcgg atttaacgcg cggcttttcg atgataattt catttctgac
     6541 cgtcgctgtc ctgacgattc cgaccggaag cgacggcgct tctttaatca ttgtgtccgg
     6601 gtctttcgac aaaaccagtt tggcgacgtc gagcggcgcc gtcttcgcgc cttttatttg
     6661 attgccgaat tgctctttgg caaccgtgac gaccgcgccc ggcggaattg tctgtaaagg
     6721 cggcagcttg ggacctttgc ccggcagcgg cgcgggaata actatcgccg gagtcggcga
     6781 cggcgtcggg ttcactccgc ccgtgttgtt gttgttatta tagttaccgc gccgacgccg
     6841 gtggtaatag ccgttgtagt tgtaataatc gtaatcatac ggcagcggat accagcaaat
     6901 cagattgccc gaccacgtaa cgacaaccaa agccggctgc caccagctgc gacgcgaccg
     6961 atacgaagca tagggcgtcc agcaccattc gccgtcgacg taaatccagc gcccgtgatg
     7021 ataagtcgcc cagccccagg gctcgtcgtt gacccaggtc cagccgtaag gcggaaccca
     7081 tcgccattga ccgtaacgat acggcgacca atcggcgtat ccggcggtcg catcccggta
     7141 aggtcgccag acccagccgt attttcgggt gaaaacccat tcgccgtaat cgcttaaatc
     7201 ctcagcgcca tacatatcac ggtcgtaata tttatcgtaa taagcgtcgc gcaggagctt
     7261 ggcgatgcgc gcgtcgcgtt ccagaaccca ttcgtcccat tcgtcggcgt agcgcgcggc
     7321 gtccgccatt tcccattcgc ccgcgttcga gccgtccaga taaacggtcg cgctgcgccc
     7381 gctgcgaagc gtgaaaccgg aattttccga ataaactctc gcttccgcgc cgtcggtcgc
     7441 cgtgacgcga atctgcgtcg aatctttatc gcccgcgtcg acgcgataca ttccggtttt
     7501 ctgcacggag acggtcgtct gcggcgcgtc aatctcgaaa taagtccggc ttttatcaaa
     7561 gctcaaaacg cgcaggctca gagttccctg cggcaggctt aaagccgcgc cttcatctct
     7621 taaagttgta atttttaaat aagaattttc cgccaggcgc aaaaacgaat cgcggtcgaa
     7681 ttgaatctcg atgcgggcgt ttaattcggt cgcaatctgg tcgccttcga caataggtaa
     7741 atttaatgca gcgcgctccc aatcgtcgct gtcggcgcgc ttgatttgaa cgtcgccgcg
     7801 cagaaaactg attcgcgcca cgcgcgccgt gacttccggt atgtaatcgt cggctgcggc
     7861 ggcgttcgga ttggcaaaaa cggtatcaaa taacaaaaga ggcatcaaca gaacgaatgc
     7921 ccatagaaca ttggctaaac gcatatactt tctccttgct tatttcgtat ctaacctgct
     7981 ttgtggaaga gaaagctgtt tgaaaagctt cagctttagc tcgcctgaca gcaaaagtta
     8041 tgcccttgat ttaaagcatt tgctgcgacc aatttattcg atttccgcgc attttccaag
     8101 cgatttttgc gattccgtat tcgatatagt tcgcgcctga tacgaaatgg tgtaaaacgg
     8161 tcggaaaact tccggaaatc actttagtga aaacaatcac aaacccgtca atctgcatcc
     8221 ttgaccgcta aaatcaggcg tgatatgctt gattcttttg agagcaggcg atttttttcg
     8281 ctgccgacag taagcaattc atactttaga aattaattat ttatggtcgt tttagctttt
     8341 gccgaaagta gtgcaattca gcttgtgccg gatggcacaa tcctgattca catcgcgctg
     8401 attctgatta tgatttgggt tctgaaccgc acgtttttcc gcccgattaa ccgcgttatc
     8461 gcggcgcgcg agaaaaacaa gggcgggcgt ttcggtgaag ccgaagaaat tttgcggcag
     8521 gttgccgaga agaacgcgcg ttttgaaaac gtgatgcgcg aggcgcgtct ggaaggctac
     8581 aataaaatcg aaaccgagcg cggcgcggcg ctgaccgaga aacaggcaaa ggtcgaaacg
     8641 gtcaaggcgg aagtcgagca gaaggtggcg gtcgaaaaag acgctctggc tcggcaagcc
     8701 gaagaagcgc gccggcaaat cgcggcggaa gccagagtta tggcggaaaa aatcagctct
     8761 aatatcttaa aataagtttt ggcgtttaat ttgaaatttc agatttcaga tttcaaatca
     8821 atttatgttt gcatctattt ttagcttcat ttttattttc gcgagcggcg gcgagcattc
     8881 ggcggcgggc ggcggatttg ccgaatggtg gcacacgcac gccgacccgt atctcaatta
     8941 tccgggtttt gaagcgtgga agtttatcaa tctggcgatt ttcgccgccg tgatggctta
     9001 tcttctgaaa aagcctctgt ctaacgcttt caaaacaaga cgcgagcaga ttcgcgccga
     9061 attgattcgc gccgaacagg aacgccgcgc cgccgaagca cgccttattg aaacggaatc
     9121 gaagctggcg cgcctcgatg tggaaagaga aaacgttttg accaaagccg agcgcgaagc
     9181 cgagctggaa agacagcgca tcgccgagca gaccgaattt gaaatcacga agctgcgcga
     9241 gcaagccgaa aacgagatca gccgcacggc gcagcaagcc aaacaggagc ttcgccggtt
     9301 gtcagccgaa gagacgattc gtctggcgga
//
LOCUS       DASWNE010000997         2068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_597987, whole genome shotgun sequence.
ACCESSION   DASWNE010000997 DASWNE010000000
VERSION     DASWNE010000997.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597987"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1439)
                     /locus_tag="VGB68_19255"
     CDS             complement(<1..1439)
                     /locus_tag="VGB68_19255"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0051443.1"
                     /translation="MSFDKTKYMRNAERFLAQGKIRQAIGEYKRIVENDAKDFSILNM
                     LGDLCVKAQDTQEAVKCYTQVAEHYAKQGFAQKAIAIYNKISRIKPESVEVSAKLAEL
                     YHAKGSVAEARAHYTALADHYQKKGNKPEALAVWKQIAELDPNNTEIYLKIAESCLQD
                     EQKDEAANAFTDAGNRFAAKKQYESATTAFSRALEIKPSNLVALNGLVNAQIGSGFAD
                     DAAKTLEGMLEKQPYNREILNLLVNCYLDVDNSLEAEKAVIKLVEQEAANYPKFLDVT
                     KSYLKNNDLTAAARVLSLTSEHLLLGGQADEFGIWINEILAKNPEQLDALRLLARYHS
                     WQRDETELKQSLERLAESARLNESVEDERYAISQLILMVPQESSYPQRMQEINEQYGF
                     AESAGEEFTPVISREENVEIPTFESFEVTEARGNGGNAAGLYLEDYAEFKPDEHFQYE
                     KIYQNGSSNGFGYEYNGENAIPLVVTEFD"
     gene            1701..>2068
                     /locus_tag="VGB68_19260"
     CDS             1701..>2068
                     /locus_tag="VGB68_19260"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY0051444.1"
                     /translation="MQALILAGGKGTRLRPLTVYTPKPIVPVLNRSFLLYQIEILRRA
                     GIKEIILSLNYQPDKIRQLLGDGQDYGVHLRYVTEPMPMGTAGAYKFAASEIRDATVV
                     FNGDILTDIDISRVVKQHQER"
BASE COUNT          445 a          525 c          509 g          589 t
ORIGIN      
        1 atgtcgaact ccgtgaccac gagcggaatc gcgttttcgc cgttgtattc gtaaccgaaa
       61 ccgtttgacg agccgttttg ataaattttt tcgtattgga aatgctcgtc cggtttaaac
      121 tcggcgtaat cttccaagta caaccccgca gcgtttccgc cgtttccgcg cgcttcggtg
      181 acctcaaagc tttcaaaggt cggaatttcg acgttttctt cgcggctgat taccggcgta
      241 aattcttcgc ccgcagattc ggcaaaaccg tattgctcgt tgatttcctg catccgctgc
      301 gggtaggaag attcctgcgg aaccatcaaa atcaactgcg aaatcgcgta tcgctcgtct
      361 tcgaccgatt cgttcaggcg cgccgattca gccaatcttt ccagcgattg tttcagctcg
      421 gtttcgtcgc gctgccagct gtgatagcga gccagcagcc tgagcgcgtc gagctgttcg
      481 gggtttttcg ctaaaatctc gtttatccag atgccgaatt catcggcttg cccgccgagc
      541 aaaagatgtt cggacgtcaa ggaaagaacg cgcgcggcgg ctgtcaaatc gttgtttttg
      601 agatagcttt tcgtaacgtc cagaaatttc ggataatttg ccgcttcctg ctcgaccagt
      661 ttgataaccg ctttttccgc ttccagcgaa ttatccacgt ccaggtaaca attgaccagc
      721 agattgagaa tctcgcggtt gtaaggctgt ttttcgagca ttccttccag cgttttcgcg
      781 gcgtcgtcgg caaaacccga accgatttga gcattgacca agccgttcag cgccaccaga
      841 ttgctcggct tgatttccaa agctctggaa aacgccgtcg tcgccgattc gtattgtttt
      901 ttcgccgcga agcgatttcc ggcgtcggta aaggcgttgg cggcttcgtc tttctgttcg
      961 tcctgcaggc agctttcggc gattttcaga taaatttcgg tgttgttcgg gtcgagctcg
     1021 gcgatttgtt tccagacggc gagggcttcc ggtttattgc cctttttctg gtaatggtcg
     1081 gctagagcgg tataatgagc gcgcgcctcg gcaaccgagc ctttggcgtg ataaagctcg
     1141 gcgagctttg ccgaaacttc caccgattcg ggcttgatgc gcgagatttt gttgtaaatc
     1201 gcaatggctt tctgggcaaa accttgtttc gcataatgtt cggcaacctg cgtgtagcat
     1261 ttaacggctt cctgcgtgtc ctgcgccttg acgcacaaat cgccgagcat attcaggatg
     1321 ctgaaatctt tcgcatcatt ttcgacaatc cgcttgtatt cgccgattgc ctgacgaatc
     1381 ttgccttgcg ccagaaaacg ctcggcgttt cgcatatatt ttgttttatc gaaactcatt
     1441 tgattaattt ttgacaggaa aatactgact aaacttggaa gagccgctgc aatttcagag
     1501 gataaaaatg cagggcggaa gtctgacttt ccctgcaatt tcaaagctat cacgttttga
     1561 caaaaagtgt caacggtttt taacaagttt tgttaggttt tatttcggaa tttacagtaa
     1621 ataaattttc tgcgcgcccg tcggctgaaa tcgttctccg cattactgtt ttcggctcgc
     1681 ttgatgttaa aattttcgtt atgcaagcct tgattctggc gggcggcaaa ggcacgcgcc
     1741 tgcgcccgct gacggtttat acgccgaaac ctatcgttcc cgttctgaat cgctcttttc
     1801 tgctttatca aatcgaaatt ctgcggcgcg cgggaatcaa agaaataatt ctttcgctca
     1861 attaccagcc ggacaaaatc cggcagctgc tcggcgacgg tcaggattac ggcgttcatc
     1921 tgcgttacgt gaccgagccg atgccgatgg gaaccgccgg cgcatataaa ttcgccgcca
     1981 gcgaaattcg cgatgcgacc gtggttttta acggtgacat attaaccgac atagatattt
     2041 ccagagtcgt taaacaacat caggagcg
//
LOCUS       DASWNE010000998         1942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_344084, whole genome shotgun sequence.
ACCESSION   DASWNE010000998 DASWNE010000000
VERSION     DASWNE010000998.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344084"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..>1942
                     /gene="nuoL"
                     /locus_tag="VGB68_19265"
     CDS             60..>1942
                     /gene="nuoL"
                     /locus_tag="VGB68_19265"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEY0051445.1"
                     /translation="MTENNFLSLIIFAPLAGAVLNWILGGWLKIRNETLSGTIACAAV
                     GVSCVVAFIIAFVDHGGALHADGPILDHLWTWIQVGGFRADFGLGMDRLSGIYACFIT
                     FVGLLIHIFATGYMHGDKGFYRFFAYLNLFMFSMLTLILADNFLLMFVGWEGVGLCSY
                     LLIGYYIKRDEARRAAKKAFVMNRIGDWGVLIGVFLIFSLTGSISFYDKTVDGAQVQS
                     SLNLIAAMPSDSFTWGAIVAGGITSIAVLLFIGACGKSAQIPLYTWLPDAMAGPTPVS
                     ALIHAATMVTAGVYMVVRCNGIYQNAPTAMLIVAVIGAATAIFAATIGLAQNDIKKVL
                     AYSTVSQLGYMFLACGVGAFIAAIFHVMTHAFFKALLFLGSGSVIHGMHHEQDMRRMG
                     NLRKYMPITFITMMAGWLAICGIPIFAGFFSKDEILYKTFAATNATAEATVLGVHVNM
                     ILWIVGLLTAVLTAVYMTRMMWMTFFGEQRFTLALPDGNHPADAAHAEENAFARGDAH
                     DAETTHGDAHATAHRAHDEERDSAHDDHDDEHHHHVDENFKPHESPWMMWVPLAVLAV
                     LSTFGGLVGVPYALSGGAIPNAFEHTLEPIIAKVGHKEAGHGAAEKKEVKHFYASSSE
                     STTG"
BASE COUNT          381 a          507 c          564 g          490 t
ORIGIN      
        1 aattcattag gaattttgtg aaagataatt caagcaagga atagtttcct tataataaaa
       61 tgactgaaaa caatttttta agtctaataa ttttcgctcc gctcgccggc gcggttttga
      121 actggattct cggcggctgg ctgaaaatca gaaacgaaac cttaagcggc acgatcgcct
      181 gcgcggcggt cggcgtttcg tgcgtcgtgg cgtttatcat cgcttttgtc gaccacggcg
      241 gcgcgcttca cgccgacgga ccgattctcg accatctgtg gacgtggatt caggtcggcg
      301 gttttcgcgc cgatttcggt ctgggaatgg accggctttc gggaatttac gcctgtttca
      361 ttacgtttgt cggcttgctg attcacattt tcgcgaccgg ctatatgcac ggcgataagg
      421 gattctatcg ctttttcgct tacctgaatt tgtttatgtt ctcgatgctg acgctcattc
      481 tggcggataa ttttctgctg atgttcgtcg gctgggaagg cgtcggtttg tgctcttacc
      541 tgctcatcgg ctattacatc aagcgcgacg aggcgcggcg cgcggcgaaa aaagcgtttg
      601 tcatgaaccg catcggcgac tggggcgtgc tgataggcgt gtttctgatt ttctcgctga
      661 ccggttcgat ttcgttttac gacaagacgg ttgacggcgc gcaggtgcaa agctcgctga
      721 atttaatcgc ggcgatgcca agcgattcgt ttacgtgggg cgcaatcgtc gcgggcggaa
      781 tcacttcgat tgcggttttg ctttttatcg gcgcgtgcgg aaaatcggcg cagattccgc
      841 tttacacgtg gctgccggac gcgatggctg gaccgacgcc cgtgtcggct ctgattcacg
      901 cggcgacgat ggtcacggcg ggcgtttata tggtcgtgcg ctgtaacggg atttaccaga
      961 acgcgccgac ggcgatgctg atcgtcgccg tcatcggcgc ggcgacggcg atttttgccg
     1021 cgaccatcgg cttggcgcaa aacgacatca aaaaagttct cgcttattcg accgtctcgc
     1081 agctcggtta tatgtttctc gcctgcggcg tcggcgcgtt tatcgcggcg attttccacg
     1141 tcatgacgca cgcttttttc aaagcgcttt tatttctggg ttcgggctcg gtcattcacg
     1201 gaatgcacca cgaacaggat atgcgccgga tgggaaattt gcgcaaatat atgccgatta
     1261 cgtttatcac gatgatggcg ggctggctgg cgatttgcgg aatcccgatt tttgccggat
     1321 ttttctccaa agacgaaatt ctctataaaa ctttcgcggc gaccaacgcg accgccgagg
     1381 cgaccgtttt gggcgttcac gtcaatatga ttttgtggat tgtcggcttg ctgacggcag
     1441 ttctgaccgc cgtttatatg acgcggatga tgtggatgac gtttttcggc gagcagcgtt
     1501 ttacattggc tttgcctgac ggaaatcatc ctgcggacgc ggcgcacgcc gaagaaaacg
     1561 cttttgcccg cggcgacgcg cacgatgccg aaacgacgca cggcgacgct cacgcgacgg
     1621 cgcaccgcgc gcacgacgaa gagcgcgatt cggcgcacga cgaccacgac gacgaacatc
     1681 atcaccacgt cgacgagaat ttcaagccgc acgaatcgcc ctggatgatg tgggttccgc
     1741 tggcggtgct cgccgttctt tcgactttcg gcggtttggt cggcgttccg tatgccttga
     1801 gcggcggcgc gattccgaat gctttcgaac acacgctcga accgattatc gccaaagtcg
     1861 ggcacaagga agccggacac ggcgcggcgg agaaaaaaga agtcaaacat ttctacgctt
     1921 cctcgtccga atcgacgacc gg
//
LOCUS       DASWNE010000999         2099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_455155, whole genome shotgun sequence.
ACCESSION   DASWNE010000999 DASWNE010000000
VERSION     DASWNE010000999.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455155"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="VGB68_19270"
     CDS             complement(<1..842)
                     /locus_tag="VGB68_19270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051446.1"
                     /translation="MSTTKINFLIAFAALILVCQACGGLGRNENRVVFNGTTNSSAST
                     STTSNDKKKTDTGTTDSDDVSTSGEERVKPAAGKGNVQGRVLYNDQPAEGIEVKICEN
                     FSTIMGIRCDGKTQTAKTDKDGVFVLADLEPKTYGGLTAKVFKSDYYVYPQEGIMTAQ
                     KFAVETDKTIFARDIHLFKDDVKITNPKAGAKVDAKNLELKWDAYPDAAYYKISLYPD
                     AGGLPPLSNERVDEPTYAVTEELTNGKYRIRVEAYNAGDRKLAESSNEIKFTVTGGAE
                     PEAKP"
     gene            1098..1592
                     /locus_tag="VGB68_19275"
     CDS             1098..1592
                     /locus_tag="VGB68_19275"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEY0051447.1"
                     /translation="MDIFWEELTSGLPDSRQLVHVVIRLIAATLLGAFIGIQRERVGK
                     PAGLRTHILVTLGTCVFVLSGSGYGMSSDGLSRVIQGVVTGIGFIGAGSILKLNEERD
                     IQGLTTAAGVWMAAAIGVAVGLGALGLAILSTVLTLLILTMVGKIEFRIDKEQRAKDR
                     LAKE"
     gene            1670..>2099
                     /locus_tag="VGB68_19280"
     CDS             1670..>2099
                     /locus_tag="VGB68_19280"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEY0051448.1"
                     /translation="MNLINSTGYLGIVALMFIENLFPPIPSEYIMPLAGFMAAEEKLS
                     LIGIIIAGTIGSVLGALPLYYCGRKLGAERLKKFAGRHGRWLALSGGDIERANGWFDR
                     HGGSAVLLCRLIPGIRSLISIPAGINRMNLFSFLFFTTIGA"
BASE COUNT          439 a          511 c          588 g          561 t
ORIGIN      
        1 ggcttcgctt caggttccgc gccgccggtg acggtgaatt ttatttcgtt gctgctttcg
       61 gcgagtttgc ggtcgccggc gttgtaggct tcgacacgga tgcggtattt gccgttggtt
      121 agctcttccg tgacggcgta tgtcggctca tcgactcttt cgttcgaaag cggcggaagt
      181 ccgcccgcat ccggatagag gctgattttg taataagccg cgtcggggta agcgtcccat
      241 tttaattcca gatttttggc gtctactttc gcgcccgctt tcgggttggt gattttcacg
      301 tcgtctttga agagatgaat gtcgcgggcg aaaatcgttt tgtcggtttc gacggcgaat
      361 ttctgcgcgg tcataatgcc ttcctgcggg tagacgtagt aatcggattt gaaaaccttc
      421 gccgtcaagc cgccgtaagt tttcggctcg aggtcggcga ggacgaagac gccgtcttta
      481 tcggttttgg cggtctgcgt tttcccgtcg cacctgatgc ccataatcgt gctgaaattt
      541 tcgcagattt tcacctcgat tccctcggcg ggctggtcgt tatacaaaac cctgccctgc
      601 acgttgccct tgcccgccgc gggtttcacc ctttcctcgc cgctcgtcga aacgtcgtcc
      661 gaatcggtcg tgccggtatc cgtttttttc ttgtcattcg atgtcgtaga agtagaagcg
      721 gacgaattcg tcgtgccgtt aaagacgacc cggttttcgt ttctgcccag accgccgcac
      781 gcctgacaga ctaaaatcag ggcggcgaag gcaattagga aattgatttt tgttgttgac
      841 acaaaagact cctttttgaa ttaatgacgg aaaaatttct ggatattcta gcacgaaata
      901 aatattttta aagtcgtcgc gggcgttaaa atcatttgct gtcttcagaa cagcggcaaa
      961 tacccatctc tttcggtttg ccttctcaga catacggtct ttccggttat attcgaatat
     1021 aaaattgaaa tgtaacaaag atttaacata aacttaactg agaagagcgc gcgcattata
     1081 aattacaagg aaaaaaaatg gatattttct gggaagaatt aacctccggc ttgccggatt
     1141 cgaggcagct cgttcacgtc gttatccgat tgatcgccgc gactttgctc ggcgcgttca
     1201 tcgggataca gcgcgagcgc gtcggcaaac cggcaggact cagaacgcat attctggtga
     1261 cgctcggaac ctgcgtgttc gttttgtccg gttcgggcta cgggatgagt tccgacggat
     1321 tgtcgcgcgt catacagggc gtcgtgacgg gcatcggttt tatcggcgcg ggctcgattt
     1381 tgaaattgaa cgaagaacgc gacattcagg gattgacgac ggcggcgggc gtctggatgg
     1441 cggcggcgat cggcgtcgcg gtcggcttgg gcgctctggg cttggcgata ctcagcacgg
     1501 ttctgaccct gctcattctg acgatggtcg gaaaaatcga attccgcatt gacaaggaac
     1561 aaagggcgaa agacagactg gcgaaagaat aaatcagaga agaattcttc gacgactgcg
     1621 aggctgacgg cggtcggtgt taaaataact cttgatgacg cgctggataa tgaatttaat
     1681 caactcgacg ggttatttgg gcatcgtcgc gttgatgttt atcgaaaacc ttttcccgcc
     1741 gattccgtcc gaatacatta tgccgctggc gggttttatg gctgccgaag aaaaactttc
     1801 gttaatcggg attatcatcg ccggaacgat aggctcggtg ctcggcgcgc tgccgcttta
     1861 ttattgcggc agaaagctcg gcgcggaaag gctgaaaaaa tttgccgggc ggcacggcag
     1921 atggctcgcg ctgtcgggcg gcgacatcga aagagcgaac ggctggtttg accgtcacgg
     1981 cggaagcgcc gttttgctct gccgcctcat tcccggcatt cgctcgctaa tctcgattcc
     2041 ggcgggcatc aaccggatga atttgttttc gtttttgttt tttacgacca tcggagccg
//
LOCUS       DASWNE010001000         2441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_526856, whole genome shotgun sequence.
ACCESSION   DASWNE010001000 DASWNE010000000
VERSION     DASWNE010001000.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526856"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..364)
                     /locus_tag="VGB68_19285"
     CDS             complement(179..364)
                     /locus_tag="VGB68_19285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051449.1"
                     /translation="MSKNKDKNKDNNDLEDLESRVKKAELILQLKRFELETLKVQEEI
                     RKVTEELKSTENPELTS"
     gene            complement(447..911)
                     /locus_tag="VGB68_19290"
     CDS             complement(447..911)
                     /locus_tag="VGB68_19290"
                     /inference="COORDINATES: protein motif:HMM:NF023175.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RebB family R body protein"
                     /protein_id="HEY0051450.1"
                     /translation="MTNPEKVISEQQTSDGSAPENEISNCAEIFCRAMSQLNAVALQQ
                     TEALLNIATSAAEKFHSLNSSATANKEELGALINQLKTAADNISQINQPTPQNSSAQP
                     ATSPATVLEAVEHQLSNAIGNSVNNQQQLNIIGAAILTQAAALLLSAGEKQS"
     gene            complement(969..1661)
                     /locus_tag="VGB68_19295"
     CDS             complement(969..1661)
                     /locus_tag="VGB68_19295"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEY0051451.1"
                     /translation="MKTRIRKFISLGLFMAMLLTSPQFLLAQTPRLVGELTITSAGEG
                     GFVTVNGERVVSGRSITSPSEITTSPQARARISLAQTGTILIEPNSRLNLSFANAGIS
                     GEFSSGEVTIETVPNTAVNILTADGTLTVPNRNQINIIKISKDGGRTRVNTLTGQAMF
                     NNVPVSAGEFYPQPVATTPDKTGGTRTNRSGGGNNSLIIIALLGAAGGAAVLALLASS
                     SNDDSSPVSPTS"
     gene            complement(1931..>2441)
                     /locus_tag="VGB68_19300"
     CDS             complement(1931..>2441)
                     /locus_tag="VGB68_19300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051452.1"
                     /translation="VNRFDRPRELQGATWRIPFDGRNLYVTVNHDGERVLEVFATGPI
                     SEGVGLLASKMLRGGFELKEVARSLNKVTATHAVWFNERLLTSPEQAVSECLLLTDRR
                     LKNMPASERAIGKAVGATPSAKTPEVVAYSEQAQMSNLIGECPECKGQLEHASGCDFC
                     RDCGYSKCK"
BASE COUNT          535 a          612 c          607 g          687 t
ORIGIN      
        1 tgattaacct cgcactctgc gtaaaaagcc cgaccaaaga cgcagccgtc agacgaagag
       61 gcgtttcctt tcgaaaacgc ctcttttccg ctgccttccc ttgcccgcgc tattaagttg
      121 tccggtgatt attactatgc acgagcagac ttgagttttt cttgagtcgc tgtttcgatt
      181 atgaagtcag ctccggattt tcggtagatt tcagctcttc ggtgactttt cttatttcct
      241 cctgaacctt taaggtttcc agttcgaagc gcttcaactg taagattagc tccgcttttt
      301 taactcgtga ttccaggtct tcaaggtcgt tattgtcttt atttttatct ttatttttgc
      361 tcattatccc tccttgataa ttaatcagta tattatctgt ttcgagcgtt tgccaggttt
      421 ttttcgagtc ttttaaaact gccggttcaa ctttgctttt cgcccgccga aagcagcagc
      481 gccgccgcct gcgttaaaat ggctgcgccg ataatgttta actgctgttg attattaacg
      541 ctgtttccaa tcgcgttact aagctggtgt tcgaccgctt cgagaacggt tgcgggactt
      601 gtcgccggtt gtgcggacga attttgcggc gtcggctggt tgatttggct gatattgtcg
      661 gcggcggttt tcaattgatt gattaatgcg ccgagttcct ctttattcgc ggtcgccgac
      721 gaattcagcg aatgaaattt ttcggcggcg ctcgtggcga tgtttaacaa agcttccgtc
      781 tgctgaaggg cgacggcgtt tagctgcgac atcgccctgc aaaaaatctc cgcgcaattt
      841 gaaatctcgt tttccggcgc tgagccgtct gatgtttgtt gttccgaaat caccttttca
      901 ggattggtca tctataaatc tccgttaata aacaaatcgt tttgaaattc tacggttaaa
      961 ttttatttct agctggtcgg gctgaccggc gagctatcgt cgttgcttga agaagccagt
     1021 aaagcgagga cagccgcccc gcccgccgcg cccagcagcg cgataataat aagcgagttg
     1081 ttgccgccgc cggaacgatt cgtgcgtgtt ccgccggttt tatcgggagt tgtcgcgaca
     1141 ggctgcggat aaaattctcc ggcagagacc ggaacattat taaacatcgc ctgaccagtc
     1201 agagtattga cgcgcgttct gccgccgtct tttgaaatct tgataatatt tatctgattc
     1261 cgattcggaa ccgtcaatgt tccgtcggcg gtcagaatat tgacggctgt gttcggaacg
     1321 gtttctatcg tgacttcgcc ggaagaaaat tcgccggaaa tgcccgcgtt ggcaaaagat
     1381 aaattcaacc tggaattcgg ctcaatcaaa atcgttccgg tctgagccag cgaaattctg
     1441 gctctcgcct gcggcgaagt agtaatttcc gacggcgaag ttatcgagcg accgctgacg
     1501 acgcgctcgc cgttaaccgt gacgaagccg ccttcgcctg cggaagtaat cgttaattcg
     1561 ccgaccagac gcggcgtttg agccagcaaa aactgcggcg aggtgagcag catcgccata
     1621 aacaagccga gagaaataaa tttacggatt cgggttttca tatgtttgat ttcctttcta
     1681 atgagcttgg attagttctt accaatgaat ttcgtcaatg tcaaattttt tgtgaaaatt
     1741 aaaaggtttt cgctataaag aattttcagc cgcgaaccgg cgcgaaagaa cggaaacgaa
     1801 aaattctttt atcaaaatgc ctcaagctct gccggttgcc gacttccgtt ttaattttct
     1861 cccgtcaaaa gtaaacgccc cgaatctatt tctaaattca gggcgtctta tttaacaatc
     1921 aattatacgt ttatttgcac ttggaataac cgcagtcgcg gcagaaatcg cagcctgaag
     1981 cgtgttcgag ctgtcctttg cattcgggac actcgccgat taaattcgac atctgcgcct
     2041 gctccgaata agcgacgact tccggcgttt tcgccgacgg cgtcgcgccg acggctttgc
     2101 cgatggcgcg ttccgaagcg ggcatatttt ttaaacggcg gtcggttaaa agcaggcatt
     2161 ccgagacggc ttgttcgggc gaggtcagca gtcgttcgtt aaaccagacg gcgtgcgtcg
     2221 cggtgacttt attcaagctg cgcgcaactt ccttgagctc gaagccgccg cgcagcattt
     2281 tcgaagcgag cagcccgacg ccctcgctga taggtccggt ggcgaaaact tccagcacgc
     2341 gctcgccgtc gtgattgacg gtgacgtaca ggttgcgccc gtcaaacgga atgcgccacg
     2401 ttgcgccctg caattcgcgc gggcggtcga agcgattgac c
//
LOCUS       DASWNE010001001         1551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_317056, whole genome shotgun sequence.
ACCESSION   DASWNE010001001 DASWNE010000000
VERSION     DASWNE010001001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317056"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /gene="rsmH"
                     /locus_tag="VGB68_19305"
     CDS             complement(<1..653)
                     /gene="rsmH"
                     /locus_tag="VGB68_19305"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEY0051453.1"
                     /translation="MKSNEETTENLHKSVLLAETLQYLAPAGDEIFVDATLGLGGHAE
                     AILSATEKTRVIGIDQDTEAISEAQNRLARFGERFRVFHANFSEIRKVLAQAQIEKVD
                     GVLADLGVSSLQLDSETRGFSFRFDAPLDMRMDAESGEETAAEMLETLSEFEIARIIY
                     EYGEERFSRKIARWIVERRERGESIKTTGELARLIERAVPRGGKKDRIHPATRTFQA"
     gene            complement(666..1109)
                     /locus_tag="VGB68_19310"
     CDS             complement(666..1109)
                     /locus_tag="VGB68_19310"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051454.1"
                     /translation="MLRGNYTARIDEKGRLKIPTHFRRKIEEKYDSAEVYVTSLTGES
                     VQIFPLKEWEIIEQKLSLLPTTERARTIYVTGTSFHGQESEIDNQGRVLIHPLLRKKA
                     VIYGDVAVLGYLTYLDVWNMERIERFLSENPFTEEHGALLARLGI"
BASE COUNT          338 a          402 c          335 g          476 t
ORIGIN      
        1 aaagcctgaa acgtccgcgt cgccggatga atcctgtctt ttttaccgcc gcgcggcacg
       61 gctctttcaa ttaagcgggc aagctcgccg gtcgttttga tgctctcgcc gcgttctctt
      121 ctttcgacaa tccagcgcgc gatttttctg gaaaatcttt cctcgccgta ttcgtaaatg
      181 attcgcgcga tttcaaattc ggaaagcgtc tccagcattt cggcggcggt ttcttcgccc
      241 gattcggcgt ccattcgcat atcgagcggc gcgtcgaagc gaaaactgaa accgcgcgtt
      301 tcgctgtcga gctgcaagga ggaaacgccc aaatccgcta aaactccgtc aactttttca
      361 atctgcgcct gcgccagaac ttttctgatt tccgagaaat tcgcgtgaaa aaccctaaat
      421 ctttcgccga accttgcgag cctgttttgc gcttcggaaa tcgcttccgt gtcctggtcg
      481 atgccgatca cccgcgtctt ttccgtcgcc gacaaaatcg cttccgcgtg cccgccgagt
      541 ccgagcgtcg catcgacgaa aatctcgtcg cccgccggcg ccagatactg caaggtttcc
      601 gccaggagaa cggacttatg caggttttcg gttgtttcct cgtttgattt caaagagctt
      661 gcaccctaaa ttccgagtct ggcgagcaat gcgccgtgtt cttccgtaaa aggattttcc
      721 gacagaaatc gctcaatcct ttccatattc caaacatcca gataagtcag ataacccaaa
      781 accgcgacgt cgccgtaaat gacggctttt tttctgagca gcgggtgaat caaaacccga
      841 ccctgattat caatctcgct ttcctgaccg tgaaagcttg tgccggtcac gtaaatcgtc
      901 ctggctcttt cggttgtcgg caaaagcgat aatttttgct cgataatttc ccattcttta
      961 agcgggaaaa tttgcacact ttcacccgtc aagctcgtta cgtaaacttc cgctgaatcg
     1021 tatttttctt ctatcttacg ccggaaatgg gttgggattt tcaaccgtcc tttttcgtca
     1081 atccgcgccg tataatttcc gcgtaacatt tttctgaatt ttggggaaag tgcttaaaac
     1141 ctctccatta tcttaaacac tatttcaagg tttggctttc ttgtctaatt gaccacttta
     1201 gaccattttt gaccacttct aaggcatatt acgattattt aaaggggtat gtcaagcaat
     1261 atttttagat tttatgtttt attcaactgc ttaaactttg acgggaaatc tttgacggcg
     1321 agcggcgggt tttctaatag tattttatat ctcacatctg acaactttgc aattttgcgg
     1381 taaagttttc tttctatgaa tttcagttcg atcgaacttc tcgttttagg tcttttcgcc
     1441 gcttttgcaa atgttctggg cgggttgatt ctgctgcctt cgggcgttta ccaaaagttt
     1501 aaagccgcgc tcaaatattt gctcgctctg ggagccggat ttatgctggc g
//
LOCUS       DASWNE010001002         5740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_317093, whole genome shotgun sequence.
ACCESSION   DASWNE010001002 DASWNE010000000
VERSION     DASWNE010001002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317093"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..1004
                     /locus_tag="VGB68_19315"
     CDS             240..1004
                     /locus_tag="VGB68_19315"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEY0051455.1"
                     /translation="MKLIVLGSGTAVPHPRRSSSGYWLETAAGTLLLDCSASAVHRMA
                     QEKLDWANLDAVWISHFHLDHVGGLAPFLFGTRNATETRERVKPLRIFGAKGLRALIE
                     KFDAVNNYKLFKQPFPVEIREVEPLEKFEILKDTEAVALKTPHTGESLALHLRHKDKT
                     LVYTADTGFTMTLGDFSRGVDLLLIECSFYKNKTTEKHLELGEAMHLIRYAKPQRVVL
                     THLYPEWDAFDLVGEAEKFSPPCEVLEARDGLRLNL"
     gene            1697..2269
                     /locus_tag="VGB68_19320"
     CDS             1697..2269
                     /locus_tag="VGB68_19320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051456.1"
                     /translation="MKTKLNAAMLAAMMVLGLILVGGGTAYAQQTDAYNLGYNYGRQD
                     KRADRSSDFRRYNNEFNRSTRRDFKQGYEDGYRDAGTRNWGNVWRRNENRNDNYGQGS
                     GRTPPSWMVGSFRGYTPSNDTYTVITIGADGYITIAAENGDGYARGYYNGGRVNFDWG
                     QYRFQREGNGFRAVNLGNSNDRVYYQRISR"
     gene            complement(2372..4138)
                     /locus_tag="VGB68_19325"
     CDS             complement(2372..4138)
                     /locus_tag="VGB68_19325"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY0051457.1"
                     /translation="MVEVKAQDATPTPLPTVSPTPSPTGSPTPASTLPAVKDAQNVQP
                     ENLNGVPQIAPEYRSDDKSLPELGRVGVDMMQQKPLTLREAITMALENNKDIEVTREN
                     VRIAEFDLQSARGVYEPRFTGQTYYERSTVPNISFFTPQITKTTNSSIVGNAGFQAFV
                     PKFGTILNATVNNQRVASDNPVSIISPQYNTGFTFSVTQPILRGRSFDQNRRTIEIAK
                     RNLSLTDTQFRQRSIETIATVQRAYWDLTYALRNLQVQRDTVRDAKDQLEHNRRLVEE
                     GQLAPIDIVAAETQVANFEQAVYEALNTVAQAENNLKNLIAQNKNDALWSASLVPVDP
                     VDLDAPTTTLPEALDAALQNRPELDINQTQRDINEIDRRFYREQTKPQVDLVASYTSA
                     GVGGSLNPNFTNPLLGNCNTIPLPQQCVDAQQAQQTLLQNVGGAGTTFTDILVNRYPT
                     IRVGVQFNLPLNGNKTVKAQLGRSLVEGERLQTQREQLEQNIQVDVRNALQSVRTAEA
                     RLRSAAISRDNSQKQYESEQRKLDAGQSDIYKVLERQTALATAKSNELRAQTELNKAI
                     ADLQRATGNSLKANNVEARLRK"
     gene            complement(4321..>5740)
                     /locus_tag="VGB68_19330"
     CDS             complement(4321..>5740)
                     /locus_tag="VGB68_19330"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0051458.1"
                     /translation="ALFEKIAMDVRRIEGVTDTLTTIGGGQQQIVNSGSIYVKLSDIH
                     DRPKSQEQMMSQTRELLKNYPPELRTGVQQVQAFSGGGFRNANVQFLVSGPDLKKLEE
                     FSAKLLEKMKTIPDAVDVDSTLISGKPEVRLEVDRDRAADLGVRIGDVSQALNTLVAG
                     QEATTFNEGTDQYEVHVRAINNYRSDVEGLKRMIVPSTKLGLVTLDRLVKVSEGTGPS
                     SIERLNRQRQVTMLANTIPGGSATNITSAIDQYVKELKLPAGYSTGYVGQSKEMGKAG
                     FYFLLAIILSFVFMYIVLAAQFESFIHPVTILLTLPLSIPFGILSLLLTGQTVNIFSG
                     LGLLLLFGVVKKNAILQIDHTNGLRAKGMNRYDAIIQANRDRLRPILMTTIALVAGMI
                     PLVVSSGAGAGTNRSIGVLVVGGQSLCLLLTLLAVPVFYSLFDDLSEMKLFRRAAGAS
                     SWLFGGIKRRFTTATSSLFGKN"
BASE COUNT         1357 a         1499 c         1505 g         1379 t
ORIGIN      
        1 agagatttca ttttcaaaag cgtgccgccg acggcgagct ccatatcgtc gggatgcgcg
       61 aaaatcgcca gtatatcaac ttcactcata agataaattt accacaccga ggacagagag
      121 aaaattagac gaagatgaag acgattaagc agataaacac aaaatcacta aacatctttt
      181 tcaaaactct tgtcaatcgc cttcgtcctc gtttaatttc tttttgatat tataaagcta
      241 tgaagttaat cgttctcggt tcgggcacgg cggttccgca tccgcgcaga agcagctccg
      301 gttactggct ggaaaccgct gcgggcacgc ttttgcttga ttgctcggct tcggcggtgc
      361 atcgaatggc gcaggaaaag ctcgattggg cgaatctgga cgccgtctgg atttcgcatt
      421 ttcatctcga ccacgtcggc gggctggcgc cgtttctgtt cggcacgaga aacgcgaccg
      481 aaacgcgaga gcgcgtcaag cctctgcgca tcttcggcgc gaaaggcttg cgcgcgctta
      541 tcgaaaagtt tgacgctgta aataattaca agcttttcaa acagccgttt ccggtcgaga
      601 ttcgcgaagt cgaaccgctc gaaaaattcg aaatcctgaa agatacggaa gccgtcgctt
      661 taaaaacgcc gcacaccggc gaaagcctcg cccttcatct caggcacaag gacaaaacgc
      721 tcgtttacac ggcggacacg ggttttacga tgacgctcgg cgatttttcc agaggcgtcg
      781 atttgctttt gattgaatgt tcgttttaca aaaacaagac gaccgaaaag catctcgaac
      841 tcggcgaagc gatgcatctc atccgatacg ccaagccgca gcgcgtcgtg ctgacgcatc
      901 tgtatcccga atgggacgcg tttgatttgg tcggggaagc cgaaaaattt tcgccgccgt
      961 gcgaagtcct cgaagcgcgc gacggcttgc ggctgaattt gtaaaaaagc gctctccggc
     1021 agccgctccg cttttttatt gtcagtaatt cggattctgc atattatcat cctgctgacc
     1081 cgcaataaga taaaagtgaa gcggcttatg aaatttacgg ctgcgcggac gagcttttta
     1141 caccgctctt tattttcatc catttcgttt caggcgtccc ggcttctata gtcgcgcatt
     1201 tatacggaga aaaactggat tgtcaggcgg tatttgccgg gttatgtagg ggttggctgc
     1261 gacctggtaa acggaaagtg ggctgcaaaa agcaagaaaa gagaactaaa aactaaaata
     1321 aattttaatt ttcatttctc agttctcaca taaaacaaga ctagctggga tgatttccct
     1381 gactattttt caagccggct gctaccctaa ctcgtcgtgt ctgattacgg gagcaaattg
     1441 attctaatcg gaataattaa tttgttctgt cgggtagctg acatagccgg aaaaacttta
     1501 gccgagcgaa aggcgagaag tgagaactga gaattaagaa aggttttcgg ttctcaactc
     1561 tcacttttca cttttcactt ttcactcatt tataaatttt attgacagtg tacgggcatt
     1621 gaactaacct ttaagtatgc tgttttttag ctctcccgtt taaaaacaat agaacgaaca
     1681 atggaggtta tcgaaaatga aaacaaaatt gaatgcggcg atgctggcgg cgatgatggt
     1741 tttgggcttg attctggtcg gcggcggaac cgcttacgcg cagcagacgg acgcctataa
     1801 tctgggttac aactacgggc ggcaggacaa gcgagccgac cggagcagcg actttaggcg
     1861 ctataacaac gaatttaacc gcagcacgcg tcgagacttt aagcaaggct acgaggacgg
     1921 ttatcgcgac gccggaactc gcaactgggg caatgtctgg agacgcaacg agaatcgcaa
     1981 cgataattac ggtcagggca gcggcaggac tccgccaagc tggatggtcg gctcgtttcg
     2041 cggttacacg ccgagcaacg acacttacac tgtgattacc atcggtgctg acggctatat
     2101 taccatcgcc gccgaaaacg gagatggtta cgcgcggggt tattacaatg gcggcagagt
     2161 caatttcgac tggggacaat atcgtttcca gcgcgaaggc aacggcttcc gcgcggttaa
     2221 tctcggcaac agcaacgacc gtgtctatta ccagcgtatc agccggtaga acagaaagaa
     2281 gacaaaagta aaaaggtaaa aagtaaaaag gcaaaagtaa ggaagtagct ttcttcactt
     2341 ttgccttttg cctttttact ttcttttttt cttattttct gagtctcgct tcgacgttgt
     2401 tcgctttcag cgaatttccg gtcgcgcgct gtaaatcggc aatcgctttg ttgagttccg
     2461 tctgggcgcg cagctcgttg ctttttgccg tcgccagggc ggtctggcgt tcgagaactt
     2521 tgtaaatgtc ggattgtccg gcatcgagct tgcgctgttc cgattcgtac tgcttctgcg
     2581 aattgtcgcg cgaaatcgcc gcgcttctca aacgcgcttc ggcagtgcgc accgattgca
     2641 gagcgttgcg cacatcgacc tgaatgtttt gctcaagctg ctcgcgctgg gtttgcagcc
     2701 gctcgccctc gaccagcgaa cgtccgagct gcgctttcac cgtcttattg ccattgagcg
     2761 gcagattgaa ctgcacgccg acgcgaatcg tcgggtaacg attgaccaga atatcggtga
     2821 aagtcgttcc cgcgccgccg acgttttgca aaagcgtttg ctgagcctgc tgtgcgtcaa
     2881 cgcattgctg cggaagcgga atcgtattgc agttgcccaa aagcgggttg gtaaaattcg
     2941 gattcagact tccgccgacg ccagccgacg tgtagctcgc caccaaatcg acctgcggtt
     3001 tggtctgctc gcggtaaaaa cgacggtcga tttcgttgat atccctctgc gtctggttga
     3061 tgtccagttc gggacgattt tgcagagccg catccagcgc ctcgggcaga gtcgtcgtcg
     3121 gcgcgtccaa atcgaccggg tcgaccggca cgagcgacgc cgaccagagc gcgtcgtttt
     3181 tgttttgcgc aatcaggttt ttcagattgt tttccgcctg cgcgaccgtg ttcagcgctt
     3241 cgtaaacggc ttgctcgaaa ttggcgacct gagtttcggc ggcgacaata tcaatcggcg
     3301 cgagctgtcc ttcctcgacc agccgtcgat tgtgttcgag ctggtctttg gcatctctga
     3361 ccgtgtcgcg ctgcacctgt aaattccgga gagcgtaggt caaatcccag taagcgcgct
     3421 gaacggtcgc gatagtttcg attgagcgct ggcgaaactg cgtgtcggtc agagacagat
     3481 tgcgcttggc gatttcgatt gtccggcgat tctggtcaaa cgagcgcccg cgcaaaatcg
     3541 gctgcgtgac cgaaaacgta aaacccgtat tgtattgagg gctgataatc gaaaccggat
     3601 tgtcgctggc gactcgctga ttattgacgg tcgcgttcag aatcgttccg aatttcggca
     3661 cgaaagcctg aaaacccgcg ttgccgacaa tcgagctgtt cgtcgttttc gttatctgcg
     3721 gcgtaaaaaa gctgatgttc ggcacggtcg agcgctcgta gtaagtctgc ccggtaaaac
     3781 gcggctcgta gacgccgcgc gccgactgca aatcaaactc ggcgattcgc acgttctcgc
     3841 gcgtgacctc gatgtccttg ttgttttcca gagccatcgt aatcgcctcg cgcagagtga
     3901 gcggtttttg ctgcatcata tcgacgccga cgcgtcccaa ttccggcaga gatttatcgt
     3961 cgctgcggta ttcgggcgca atctgcggaa cgccgttcag attttccggc tgaacgttct
     4021 gcgcgtcttt tactgcgggc agcgtcgacg ccggcgtcgg cgaaccggtc ggcgaaggcg
     4081 tcggcgaaac agtcggcaaa ggcgtcggcg tcgcgtcctg cgcttttact tcgaccaaag
     4141 acagaagaga taaaattaaa atgaataaat ataaatgcct tttcataaag cctgtataaa
     4201 aacgtcaaaa tttaggattt aggattcagg attcaggatt caaaatttgg gttcttgatt
     4261 ttaatcttaa atcttaaatc ttaaatcctg aatcctgaat cctgaatcat ttaaccgaac
     4321 tcagttttta ccgaacagcg aactggtcgc cgtcgtaaac cggcgcttga tgccgccgaa
     4381 aagccacgac gaagcaccgg cggcgcggcg aaacagcttc atctcgctca aatcgtcaaa
     4441 cagcgagtag aaaaccggaa ccgccagcag cgtcaaaagc agacacaagc tttgcccgcc
     4501 gacgaccaga acgccgatgg agcggtttgt tcccgcgccc gcgcccgacg aaacgacgag
     4561 cggaatcata ccggcgacca gcgcgatcgt cgtcatcaga atcggtcgca gacggtcgcg
     4621 gttcgcctga ataatggcgt cgtaacgatt cattcctttt gcccgcaagc cgttcgtgtg
     4681 gtcaatctgc aaaatcgcgt tctttttcac cacgccgaaa agaagcagca agccgagtcc
     4741 ggaaaagatg ttgacggttt gtccggtaag cagcaggctg agaatgccga acggaatcga
     4801 gagcggcagc gtcagcaaaa tcgtcacggg gtgaataaac gattcaaact gcgccgccag
     4861 cacgatatac ataaagacaa acgacaggat aatcgccaaa agaaaataaa aaccggcttt
     4921 gcccatctcc ttcgattgac cgacgtagcc ggtcgaataa ccggcgggca gcttcagttc
     4981 cttgacatat tggtcaatcg ccgacgtgat attcgtcgcc gagccgcccg gtatcgtgtt
     5041 cgccagcatc gtcacctgac gctggcggtt caggcgctca atcgagcttg gtcccgtgcc
     5101 ttccgaaact ttgaccagac ggtcgagcgt caccaagcct aacttggtcg aaggcacgat
     5161 catccgtttt aatccttcga catccgaacg gtaattgtta attgcgcgga cgtgaacctc
     5221 gtactggtcg gttccttcgt taaaggtcgt cgcttcctga ccggcgacga gcgtattcaa
     5281 agcctgcgaa acgtcgccga tgcgcacgcc caaatccgct gcccggtcgc ggtcgacctc
     5341 cagccgcact tccggcttgc cgctgattaa agtcgaatcg acgtcaaccg cgtcgggaat
     5401 cgtcttcatt ttttcgagca gtttcgccga aaactcttcg agctttttca agtccggtcc
     5461 cgaaaccaga aactgcacgt tggcgttgcg aaaaccgccg cccgaaaacg cctgcacctg
     5521 ctgcacgccg gttctgagct ccggcgggta atttttcaaa agctcgcgcg tctggctcat
     5581 catctgttcc tgtgatttgg gtcggtcgtg aatgtccgac agcttgacgt aaatcgagcc
     5641 gctgttgacg atttgctgct gaccgccgcc gatggtcgtc aaagtatcgg tgacgccttc
     5701 aatccggcgc acgtccatcg cgattttttc aaacagcgcg
//
LOCUS       DASWNE010001003         3605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_598199, whole genome shotgun sequence.
ACCESSION   DASWNE010001003 DASWNE010000000
VERSION     DASWNE010001003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598199"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..135
                     /locus_tag="VGB68_19335"
     CDS             16..135
                     /locus_tag="VGB68_19335"
                     /inference="COORDINATES: protein motif:HMM:NF013568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051459.1"
                     /translation="MYSGFAFGFGLERMCSLMYSLDDIRLMFENDVRFLEQFK"
     gene            complement(273..629)
                     /locus_tag="VGB68_19340"
     CDS             complement(273..629)
                     /locus_tag="VGB68_19340"
                     /inference="COORDINATES: protein motif:HMM:NF022044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY0051460.1"
                     /translation="MTFGKKKHRRHKKECPQCFAYISADSRKCKFCGYTFKSSGKYWA
                     DKIKHALRIDRAFSVSREKSQKVTQNFFTRGEIEKLTRFENIHILMMVKLDDISLLDE
                     MALIELGETLMSMNKH"
     gene            1148..3241
                     /gene="pheT"
                     /locus_tag="VGB68_19345"
     CDS             1148..3241
                     /gene="pheT"
                     /locus_tag="VGB68_19345"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEY0051461.1"
                     /translation="MNISYNWLKDLIEIDLSPPDLARKLTGVGLAVEGIHKAGDDFVF
                     DIDLTSNRPDCLSHLGVSREIRAIEDSEFKIQDLNSTNNPESGILNSELVTIQDADLC
                     HRFTARIIRNVKIAPSPEWLVKRLEAVGERSINNVADITNYVMHELGQPMHSFDYNKL
                     KENRIVVRRARSGESLKTLDEIERKLDETMLAICDAEKPVAVGGVMGGFDSGITEETT
                     DVLLEVAYFKRENIRQTSRKLNLATEASYRFERGVDIENLVRASNRAAQLICELAGGE
                     AGEFVDVYPTRFSPNEIESKDISYAVRRLTGLDVEETEILKILSALGIERKNSTSSSG
                     QPALFTSPSWRHDLAIEEDLVEEVARIVGYDKIGEELPASQSAGEYQATEPRKKHLRR
                     ALADLGFDEAISYSFIDTRHDETFALVENLTDENAEEKFVTLQDSIIEGAVRMRPSLL
                     SGLLDAVRTNFNHQQKNVRLFELGKVFAATANEDSLPKEQELFALVLSGSETLENKAM
                     PVREFDFYDAKGALETAIEAINLPALEFAAKDVRHLRRGQSAEIRAAGRAVGTIGRLN
                     EEIAAAYKFRQPVFVAEIDLQTLLQAKQKDVLYRPLSVYPSIQRDVSVLVKRGVSFAE
                     IKQTVEAQGFELLRKVEFVDVYEGKGMADDERSITIRLEYRSDERTLTEPEAEAVHAQ
                     ILQTLERDLDAKQRF"
BASE COUNT          973 a          809 c          891 g          932 t
ORIGIN      
        1 agcgtcgacc cgcaaattta ttctggcttc gccttcggct tcgggctgga aagaatgtgc
       61 tcgctgatgt attcgctcga cgacatccgt ttgatgttcg aaaacgacgt gagattttta
      121 gaacaattca agtagtgaaa atcccggata aatcgaatga gcggcggcag attagatgtc
      181 ttaaatgtaa ttgcgcccgc gggagatttg ttttacaaga cactaaaaaa gcgccggtta
      241 aatattaatt tcaggcgatg ttcagaaaat gtttaatgtt tattcatcga cattaacgtc
      301 tcgcccagct caatcaaagc catttcgtcg agaagcgaaa tatcgtccaa tttgaccatc
      361 attaaaatat gaatgttttc aaaccgggtg agcttttcga tttcgccgcg cgtgaagaaa
      421 ttctgcgtca ccttttgcga tttttcccgg ctgaccgaaa aagcgcggtc gattcttaac
      481 gcgtgtttaa ttttatctgc ccagtatttg ccggaagatt tgaatgtata gccgcaaaac
      541 ttgcattttc ttgaatcggc gctgatatag gcgaaacatt gcggacattc ttttttatgt
      601 cggcggtgtt tcttttttcc gaaggtcata aaacttggac gaatattcgc cgatttaccc
      661 ggaatttaag gctttcgggt aaatttcaca cttgagatat tttatttaca aaactacaaa
      721 gaattaatgg aaaatatttt tcagtaactc ttcaaattat aaagcttgaa aaatcgtcgc
      781 aaacgattta aaaagcgtaa agcacgcctc aaataaaatt ttccggattt ttggaataac
      841 tcaaactata cgcggtcttt tgccttttgt atatcaaaaa ttcagaatat tttgtcggct
      901 tttgcaaagg atttgaagag attgccggtc gcgctcgccg gcgcgaatcg ttgattgata
      961 aataaaaaaa ctcgccatta tatcaatgac gagtttttta ttataaagct ttccgattcg
     1021 cctgtgcgac ttgaaacata gtaaagcccg gggcaggttt gcgtcagctg tactattata
     1081 gattataata ggcattttga attttagaag caataaaaat tttgaatcat attcatatct
     1141 ttagtttatg aacataagtt acaactggtt aaaggattta attgaaatag atttatcgcc
     1201 gccggatttg gcgcggaagc tgacgggcgt cggcttggcg gtcgaaggca ttcacaaagc
     1261 gggcgacgat ttcgtgtttg atattgattt gacttccaat cgtccggatt gtctttcgca
     1321 tctgggcgtc agccgcgaaa ttcgtgcgat tgaagattca gaattcaaga ttcaggattt
     1381 gaattcgaca aataatcctg aatccggaat cttgaattct gaattggtca cgattcagga
     1441 cgcggatttg tgtcaccgct ttacggcgcg gattattcga aatgtaaaaa tcgcgccgtc
     1501 gcccgaatgg ctggtcaaac gcctcgaagc cgtcggcgag cgttcgatta acaacgtcgc
     1561 cgacattacg aattacgtaa tgcacgagct cgggcagccg atgcactcgt ttgattacaa
     1621 caaattaaag gaaaatcgaa tcgtcgtgcg ccgcgcgcgc agcggcgaga gtctgaaaac
     1681 tctggacgaa atcgagcgca aattggatga aacgatgctg gcgatttgcg acgccgaaaa
     1741 gccggtcgcc gtcggcggcg taatgggcgg tttcgattcg ggaatcacgg aagaaacgac
     1801 cgacgttttg ctcgaggtcg cctatttcaa acgcgaaaat attcgccaaa cttcccgaaa
     1861 actcaatttg gcgaccgaag ccagctatcg tttcgagcgc ggcgtggaca tcgaaaatct
     1921 ggttcgcgcc tcaaaccgcg ccgcgcagtt aatctgcgag ctggcgggcg gcgaagcggg
     1981 cgaattcgtc gatgtttacc cgacgcggtt ttcgccgaat gaaatcgaat cgaaagatat
     2041 ttcctatgcg gtcaggcgct tgaccggact ggacgtcgaa gaaaccgaaa ttttgaaaat
     2101 tttgtcggct ttaggcatcg agcggaaaaa ctcgacgtcc agctccgggc agccggcgct
     2161 ttttacttct ccgagctggc ggcacgacct cgccatcgaa gaagatttgg tcgaagaagt
     2221 agcgcgcatc gtcggttacg acaaaatcgg cgaggagctt ccggcttcac aaagcgcggg
     2281 tgaatatcag gcgaccgagc cgcgcaaaaa acatctgcgc cgcgcgcttg ccgacctcgg
     2341 ttttgacgaa gctatctctt acagcttcat cgacacgcgc cacgacgaaa cttttgcgct
     2401 ggttgaaaat ctgacggacg aaaacgccga agaaaaattc gtgaccttac aggattcgat
     2461 tatcgaaggc gcggtgcgaa tgcgtccaag cctgctgtcc ggtttgcttg acgcggtgcg
     2521 gacgaatttc aatcaccagc aaaaaaacgt gcggcttttt gagctcggca aagttttcgc
     2581 cgcgacggct aacgaagaca gtctgccgaa agaacaggag cttttcgcgc tcgtcctgag
     2641 cggcagcgaa acgctggaaa acaaggcgat gcccgtccgc gaattcgatt tttacgatgc
     2701 gaagggcgcg ctggaaacgg cgattgaagc catcaatctg cccgcgctcg aatttgcggc
     2761 gaaagacgtc aggcatctgc ggcgcggtca atcggcggag attcgcgccg ccggcagagc
     2821 ggtcggcaca atcggtcgct taaatgagga aatcgcggcg gcttataaat ttcgacagcc
     2881 ggttttcgtc gccgaaatcg atttgcagac gcttttgcag gcgaaacaaa aagacgtttt
     2941 atatcgcccg ctttcggttt atccttcgat tcagcgcgac gtttccgtgc tggtcaagcg
     3001 cggcgtgagc tttgccgaaa tcaagcaaac cgtcgaagcg caaggttttg aattgcttcg
     3061 caaagtcgag tttgtcgacg tttacgaagg caaaggaatg gcggacgacg agcgctcaat
     3121 cacgattcgg ctcgaatatc gaagcgacga gcgaaccttg accgaaccgg aagccgaagc
     3181 cgttcacgcg cagattctac agactctcga acgcgatttg gacgcaaagc agagatttta
     3241 atatgtaata aaataaagct gcgcagcaaa gaatgtaggg gcacgcccgc gtgcctgccc
     3301 aatgagcgag agcgaataag cgtttagaat atattcaaca ttgaatttat ttaatatcga
     3361 ttagcgtcca ttcggacgct ttgggcaggc acaagacctg ccctacattc cttgcgtcgc
     3421 gtatcttttg cattacacga ccgatactgc gaatgaaact tttatcttac acttgaaagt
     3481 ttgatatttc ggttgaaagt tttgttaaat aagccgaaag aaatttatct tcgccgaatt
     3541 gcctttttag cggctttaca ttaaatattt tctaaaaact taagaaaaca cttgaagttt
     3601 ttgcg
//
LOCUS       DASWNE010001004         1637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_598227, whole genome shotgun sequence.
ACCESSION   DASWNE010001004 DASWNE010000000
VERSION     DASWNE010001004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598227"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(244..>1637)
                     /locus_tag="VGB68_19350"
     CDS             complement(244..>1637)
                     /locus_tag="VGB68_19350"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEY0051462.1"
                     /translation="TGENARRISNFGFHPSWSPDGKKVAVSDKAAAIHTVHTVPNSAL
                     WEIDVATGNKRQIETKGDAIMPSWSPSGSRIAFWFVADGKLGEIATIPAAGGEPVVVA
                     GDPASDWNPVWSPDGKYLYFASDRSGSMSFWRVAIDEETGAALGAAESVQTPSKYCRH
                     ITFSRDGKTLGYVRYESQSNLQAIAFDPKSLKTTGEVNWITRGDKEIGNPDLAPNGET
                     FVVRQPTRTQEDLVIFDKNGGNWRSLMNDKFRERIPRWSPDGSQIAFYTDRSGKYQIW
                     TINPDGSNLRQITFTEKMGANAPVYSPDGLRMAFTELEINTQSSFILDLTKPWNEQTP
                     QALPPMANNKSFSVRDWSNDGKRLLLIYFEPDGDEHGIGVFNLETSAYEKMTESGSMP
                     FWLNDNRHFIYTDRNAIFLCDTQTKKISELYKPFAYELQHANISPDNKMIFFRYLQVD
                     ADVWLIDAAAQNQ"
BASE COUNT          364 a          424 c          439 g          410 t
ORIGIN      
        1 ggcaaaaacg ccgagcgcca gaaccgagac catcaaagat gcttttaata atttcattgt
       61 aagttagaca aattttctgc gaaaaagttc ccgtaaaatc agaatctaac ggaaaaattc
      121 ctttccggct ctttgcgaaa aaccttgcgc tctttgcggt taaagaaaga tttttacccg
      181 cggaagcaac aaagttttat gacgcgaaga aagcagaggc gtaacttatt tttgaaattg
      241 tttttattga ttctgcgcgg cggcgtcaat cagccagaca tcggcgtcga cctgtaaata
      301 acgaaagaaa atcattttgt tgtcgggcga gatgttggcg tgctgaagct cgtaggcgaa
      361 cggcttgtaa agctcggata tttttttcgt ctgcgtatcg cacagaaaaa tcgcgttgcg
      421 gtcggtgtaa ataaaatggc gattgtcgtt gagccagaag ggcatcgagc cgctttcggt
      481 cattttttcg taagcgctgg tttccagatt gaaaacgccg atgccgtgct cgtcgccgtc
      541 cggctcgaaa taaataagca gtaatctttt gccgtcattc gaccagtcgc gcacggaaaa
      601 agatttattg tttgccatcg gcggcagcgc ctgcggcgtt tgttcgttcc agggtttggt
      661 caaatccaaa ataaaagaag attgcgtgtt gatttcaagc tcggtgaaag ccatccgcaa
      721 accgtccggc gagtaaacgg gcgcgttggc gcccattttt tccgtaaaag taatctggcg
      781 gagattcgag ccgtccggat ttatcgtcca tatttggtat ttcccgctgc ggtccgtata
      841 aaacgcaatc tgcgaaccgt cgggcgacca gcgcggaatt ctctcgcgga atttatcgtt
      901 catcaggctg cgccagttgc cgccgttttt atcgaaaatc acgaggtctt cctgcgtgcg
      961 cgtcggctgg cggacgacga acgtctcgcc gttcggcgct aagtcagggt tgccgatttc
     1021 cttgtcgccg cgtgtaatcc agttgacttc gccagtcgtt ttcaggcttt tcgggtcaaa
     1081 agcgattgcc tgcaaattgg attgactttc gtagcgaacg tagccgagcg ttttgccgtc
     1141 gcgcgaaaaa gtaatgtggc gacagtattt ggaaggcgtc tggacggatt ccgccgcgcc
     1201 gagcgccgcg cccgtttctt catcgatcgc cacgcgccag aagctcatac ttccgctgcg
     1261 gtcgctggcg aaatacagat attttccgtc cggcgaccag accggattcc aatccgaagc
     1321 cgggtcgccg gcgaccacca ccggttcgcc gcccgctgcc ggaatcgtcg caatctcgcc
     1381 gagttttcca tccgccacaa accagaaagc gatgcgagaa ccgctcggcg accagctcgg
     1441 cataatcgcg tcgcctttgg tttcaatctg ccgcttgttt ccggtggcga cgtcaatctc
     1501 ccagagcgcg ctgttcggaa ccgtgtgaac cgtgtgaatg gcggcggctt tatcgctgac
     1561 ggcgaccttt tttccgtccg gcgaccacga cggatgaaag ccgaaattgg aaatgcggcg
     1621 cgcgttttct ccggttt
//
LOCUS       DASWNE010001005         4033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_173092, whole genome shotgun sequence.
ACCESSION   DASWNE010001005 DASWNE010000000
VERSION     DASWNE010001005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173092"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VGB68_19355"
     CDS             <1..538
                     /locus_tag="VGB68_19355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051463.1"
                     /translation="EDKKNLANELLKFVRQHTNRRLGIKHGGVREREGFSWTKERKAE
                     FFEMVERIPKIERKKNGKRETLYLWEYIYEQFDSRGHDAKESLKSEQGFELIPLELLN
                     ETFDKWERYEETFTNIDAEDSPKMFLFRQALHLLNFPSEHFNKRENKFIPYKRATLQK
                     YYDAGKEVFQDSDNQDKS"
     gene            713..916
                     /locus_tag="VGB68_19360"
     CDS             713..916
                     /locus_tag="VGB68_19360"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY0051464.1"
                     /translation="MDKKILVADEVAEILRVDKQRVYELVRANQIPVIRLGERQYRFS
                     AQAIEEFLRCGGTKKEEAEYVAN"
     gene            1099..3135
                     /locus_tag="VGB68_19365"
     CDS             1099..3135
                     /locus_tag="VGB68_19365"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0051465.1"
                     /translation="MNKLKFSEVLSRLGNLRRGSDGGYTARCPAHDDRRNSLSLREKS
                     DGGTLFYCHAGCPFENIVNALETSPGGVSTVGETEIVYDYKDEAGKTLYQNVRSAGKE
                     FRWRRIDENGRVAWNLNGARRVPYHLPELLKLTELQDVLMTEGEKDADALARRGFPAT
                     NHKNWTPEFNYLLKGKNVIIFRDHDRPGFEQAEKAARIVYRDAKAVKVVDCFPNEPVP
                     EKHGKDVSDFLQTHSLDELRELIRRTPVYQPSAEVSSTDAEGKDRQGFRVVRLFDVEE
                     KEIDWLWKPFIALGEFTILEGIEGQGKSWLCGALACAVAGGKRLPFHDAPVMQGNVLL
                     LSAEDSLSHTVKPRLISMRADTTKIFAIDEVFSLDNIKDLIKFEEIIAEYNPILIIID
                     PMFSYTGGKNLNAESDSRPIARKLIAIAQKFGCAIIGVRHLGKAKGNGDARAAGLGSV
                     AWRASARSVLLVGGDEETGEKAVCQTKNNLAQEAGFSVGFRIENGQFFWESEPSRLTK
                     EKILAQPKDDEAKAEQTEAVAFLRDALSGGERPSAEIERDARSAGVTNYGLKKAKRLL
                     GVESFKKGGTFGGERCWFMRMPSAEDEQSEIEESDNFEFRPIQSNDGDKTVSGNNLVE
                     EVENRRNQRLQASANTSSSNGSVPEVRVAALCRCSSNGFVGEDCPSCGEMIIPF"
     gene            3511..3777
                     /locus_tag="VGB68_19370"
     CDS             3511..3777
                     /locus_tag="VGB68_19370"
                     /inference="COORDINATES: protein motif:HMM:NF016330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HEY0051466.1"
                     /translation="MFILKGIDQLEKAIQRARKIRPRVEFDHFGRYRVSGSKGGYYTV
                     ICRKDERGVRAVACTCKGGERGLVCYHAAALSLHVGLARQRQIA"
BASE COUNT         1225 a          816 c         1033 g          959 t
ORIGIN      
        1 cgaggataaa aaaaatttgg cgaatgaatt attaaaattt gttcgccaac acacaaacag
       61 gcggcttggg attaaacacg gcggcgtcag agaaagggag ggatttagct ggactaagga
      121 aagaaaggca gaattttttg aaatggttga gcgtattcca aagattgaaa gaaagaaaaa
      181 cggtaagcga gaaacactct atctttggga atatatctac gaacagtttg actcgcgcgg
      241 tcacgatgcg aaggagtcct tgaaatccga gcagggtttt gaactaatac ctttagaatt
      301 gctaaacgaa actttcgaca aatgggagag atatgaagaa acttttacta atatagacgc
      361 cgaagattcg ccaaaaatgt ttttgtttag acaggctttg cacctcctaa actttccgag
      421 tgaacatttt aataaacgag aaaacaagtt tattccatac aaaagagcaa ctcttcaaaa
      481 gtattatgac gcggggaaag aggtttttca ggattcagat aatcaagata aatcttgatt
      541 atctgaacat ttaaatattt tcagaatagc aaaattaaat cttgtaaatt ctccaaaata
      601 actaattttt gggcaaacaa gattttcttt gacgcttcat ttttgcgtct gtcaggcttg
      661 gattcgttga gattattctc agcgaatctt ttttattttg gaactgaaaa ttatggacaa
      721 aaaaatttta gtagctgatg aagttgccga aatactgaga gttgataagc agcgcgtcta
      781 tgaactcgtg cgggcaaatc aaattccggt tattcggctc ggcgaaaggc agtatagatt
      841 ttcggcgcag gcgattgaag aatttttgcg ctgcggcggg acaaaaaagg aggaagctga
      901 atatgttgca aattaaacaa cggaagcggc ggtattgtaa caccgccgga aaaagaaaag
      961 ccgcgtttca cggacgcggc ttcaaatcaa atttcataac taaacaaaaa gttatgaatc
     1021 taaccaaccc aattataaga cagaacttga aaaacgagaa aaatttttcg cgtcggcgaa
     1081 cgaagggagg tgaaagctat gaataagctg aaatttagtg aagtgctttc acggctcggt
     1141 aacttgagaa ggggcagcga cggcggctat acggcgcgtt gccctgctca cgacgaccga
     1201 agaaattcgc tgtcgcttcg cgaaaaatca gacggcggaa ctctgtttta ttgtcacgcc
     1261 ggttgcccgt ttgaaaatat agtcaacgct ctggaaactt cacccggcgg cgtctcgacg
     1321 gtcggcgaga cagaaatcgt ttacgactat aaagacgaag ctggaaagac actttatcaa
     1381 aatgtccgca gcgccggaaa agaatttcgg tggcgacgaa ttgacgaaaa cgggcgtgta
     1441 gcttggaatc tgaacggcgc gcgtcgcgtc ccgtatcatt tgccggaatt gttgaagttg
     1501 actgaacttc aagatgtatt gatgacggaa ggtgagaaag acgccgacgc gctggcgcgg
     1561 cgcggatttc cggcgacgaa tcacaaaaat tggacgcctg agttcaatta tttgctcaaa
     1621 ggaaaaaacg tcatcatttt tcgtgaccac gaccgacccg gattcgagca agccgagaaa
     1681 gctgcacgaa tagtttatcg ggacgctaaa gcggtcaaag tcgttgactg ttttcctaat
     1741 gagcctgtgc cggaaaagca cggaaaggac gtttctgatt tcttacagac acatagcctt
     1801 gatgagctac gcgaactgat tcgccgcacg ccggtttacc aaccttcggc agaagtttcc
     1861 agcaccgacg ctgagggtaa ggacagacaa ggcttccggg tcgtccgact ttttgatgta
     1921 gaagagaagg aaatagattg gctctggaaa ccatttattg cgctcggcga gttcacaatt
     1981 cttgaaggaa ttgaagggca gggtaaaagt tggttgtgcg gcgcactcgc ctgcgccgtc
     2041 gccggaggca agagattacc atttcacgac gcgccggtga tgcagggcaa tgtcctgtta
     2101 ctgtcggcag aagattcgct ttcgcatacg gtaaagccgc gcttaatctc aatgcgcgcc
     2161 gacaccacga aaattttcgc cattgacgaa gttttctcgc tggacaatat caaagacctg
     2221 attaaatttg aggaaattat cgctgagtat aatccgattc tcattattat tgacccgatg
     2281 ttttcttaca cgggcggaaa aaatctcaat gccgaatccg acagccgacc catcgctcgt
     2341 aagttgattg cgatagctca gaaatttggc tgcgccatta tcggcgtccg ccatctcggt
     2401 aaggcaaaag ggaacggcga cgcccgcgct gccggattag gtagcgtcgc ttggcgggcg
     2461 tctgctcgtt cagttctact tgtcggcggc gacgaagaga caggcgagaa agccgtctgt
     2521 caaaccaaaa ataatttggc gcaggaggca ggtttttctg ttggattcag aattgagaac
     2581 ggtcagttct tttgggaaag cgagccgagc cgacttacta aggaaaagat actggctcag
     2641 ccgaaagacg acgaggcgaa agctgaacaa accgaagccg tagcatttct tcgtgacgca
     2701 ctcagcggcg gcgagcgtcc gtctgccgaa atcgaaagag acgcgcgcag cgccggtgtg
     2761 acgaattacg gtttgaagaa ggcgaaaaga cttttaggcg tcgagtcgtt caagaaggga
     2821 ggaacattcg gcggggaaag atgctggttt atgcgtatgc ccagcgctga agatgaacaa
     2881 agcgagattg aagaaagtga taattttgag tttcgaccaa ttcagtcaaa cgacggagat
     2941 aaaaccgttt ccggcaataa cttagttgaa gaagtagaaa atagaagaaa tcaacgtctt
     3001 caagccagcg ccaacacatc atcctcaaat ggcagcgtgc cggaagtcag agtggcggct
     3061 ttatgtcggt gtagttcaaa cggcttcgtt ggtgaagatt gtccaagttg cggggaaatg
     3121 attatcccgt tttgaaaaac tgaggcaaac cgaaagtgat gcgtgctgcg gagcagttga
     3181 aaacataaaa gtcaaaatgg gaagactatt tcttagccac aggcggtctt ccgggttttc
     3241 gctctttgac aagttttagg tctgattctt ttatgacgtg agagcgtccg attcgtgttg
     3301 aaggtagatt gccagaattt atcagagcaa caacacggcg caccgacacg ccaagtttat
     3361 cagcagcttc ctgagttgtt agataacctt tcataaagga attataacat agaaaaacta
     3421 ttccgcaata agcatattta atttgacaaa ctattccgaa atcggtatag ttaaagagta
     3481 gtatttattt taaagaaagg aaaaaaccaa atgtttattt tgaaagggat tgaccaatta
     3541 gaaaaggcga ttcagcgagc gcgaaagatt cgtccgcgcg tcgagttcga ccatttcggg
     3601 cgctaccgtg tttccggcag taaaggtggt tattacacag tcatttgtcg aaaagatgag
     3661 cgaggcgtgc gcgcggtcgc ctgcacctgc aaaggcggcg aaagaggtct ggtttgctat
     3721 cacgcggcgg cgctgtcact tcacgtcggt ctggctcgac aaagacaaat tgcataaatg
     3781 gcaaaggctt aggaaagcta ccaactaacc taagcccgtt atacccgaag gaaacctact
     3841 aaagaaacca acggacgcgc tcattttacc ataatgcggc gcgtcctact cgaagagaaa
     3901 ttatgagagg acaaatagtt gaaaagggaa aagataaatg gctggtgcga atttttcgcg
     3961 gcagggatgc gagcggaaaa aagctatatt tcaataaggt cattaacggt aaaaagtcgg
     4021 tcgcgcaaaa ata
//
LOCUS       DASWNE010001006         6249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_492698, whole genome shotgun sequence.
ACCESSION   DASWNE010001006 DASWNE010000000
VERSION     DASWNE010001006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6249
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492698"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="VGB68_19375"
     CDS             <1..661
                     /locus_tag="VGB68_19375"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051467.1"
                     /translation="APMCACVPTPSQTSISEVIDIKAGVGHTLVRKRDGSVWAWGSNV
                     VGQAGNGTTTAAQLPVQSNVGTGNVWIAAGLYYSHAGIPSIATFTGSNVRLYGENVNI
                     TFADVTASGTTHYSAIDPNAFASPAGFDIVDNSPAYNLTTSAFFTSANVCLKVTTEYS
                     PTRFDALRLLHQENGVFVDRTVSRDYRRREVCAGVTSFSPFVLALPVASAFDSEGGKD
                     D"
     gene            901..3435
                     /locus_tag="VGB68_19380"
     CDS             901..3435
                     /locus_tag="VGB68_19380"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY0051468.1"
                     /translation="MKFRVSQTLQRRFFGVIFVFALVGVLFGQSFAAAKLSPALRTKL
                     NGLANSAQVGMVVVAFKTNNGLQNSHLNILRSVGVLGGQTFPTLGMVAQPMTAGQVRA
                     LQNNSAVRSIWSNDRLHYYMHQARVVGGAARVETDRAFAIRNGGLPVSGAGDFSVLVI
                     DSGIDATHADLQFGTKVIQNVQTPVAAGTLPGFTPHVSIENVPNTDQSVGHGTHCAGI
                     IGGTGVRSGGLYPGVAPGAKIIGAGLGAGIFVINGLAAWEWGLANQYRYNIRVVSNSY
                     GGDGPFDPEDPITIASKMAYDRNITVVFAGGNAGPVKDTYNPLAKAPWVIGVAAGSKE
                     GDLADFSSRGMPREQRLSNSDPLDDNNAPTITAPGMGRAFESSLTRAGFTIDMISTRS
                     TSNLVANGTTNDTEIAPGMIPFYTQIHGTSMATPYVAGVVALMLDADPNLTPDEIKEI
                     LTSTASKMPGRSEWEVGAGYINAYAAVDKVFNRTRNYAHFQDVSFNAVFSEQRPAQQN
                     FHLDFNPAVSGATSTNATTFTVEAGMNVLDVFATVDSIAGGTGNLVGIRITSPSGVNY
                     STAIDYPVIGTDKRQIVVQNPEAGTWTLEVRGARGLTAAPQASSPIQLAMPGPVDGNV
                     TQIKYTLPNIADIQGHPQQAAIEAALKNRVVDIYADGTFKPNDTVTREDFARSLVLNT
                     SLRQTLSATPRFGDVSGDLARIAEAVTANGSTLRDFNYVPDGMMTGDDNFYPNAAVTR
                     IDMAIAFVRALGYDTQARNLANSDVTSNGAVISDNGQIPAELRGYVQIALDKGLFEAF
                     PAGVVEIAPGQFQVLPGPRFEPNTTVNRATLAIKLNSYRQLFTTGG"
     gene            3763..>6249
                     /locus_tag="VGB68_19385"
     CDS             3763..>6249
                     /locus_tag="VGB68_19385"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HEY0051469.1"
                     /translation="MFWSFIITVLYIPFGTAEASSNQAPGANSGSLYYRPASNFDLNL
                     TSSVAGLRAATVEQLGALNTLKTSTNASNMTVRWNAFGGSPDVVYDFASQPFSGTPEE
                     AGRAFIQQNAAVFGVGSLNDLKLFSQREALGGYLLRFGQTFNGIDVKDGGIGIVMNAN
                     KQVIMASGPFFRDVNVNTTPSISADQARATVNSDLAQYSNSIVSSASNLFQPALNVVS
                     QQTQIVNSIQPKLGIYPTADGYKLVWKVAKMSTNPFGLYAYSIDAHTGEIVARKDFVK
                     FQQNPVHPFTADIYPKYPDITPELKDQSIIKDCLNEQNVMAPCGQQRVKLRSFDQQNV
                     VTGLNGTLTGTHALVNNVLATKQPFAQAALGTWHFRKDDPTAFEGRTNEQDQYAEPAE
                     HQDEINAFFFVTYLLEYVDYLHVAGDNTNGLQGRFPDEYPNKTIPLPATVHFPNIYAV
                     LPLLGCPANCPVPSPTDPDLSKKLLGLDNAIAVNVTSLIEEVTAEKSPVVVNPTFYGH
                     GYLLNDLALEGTVPYHEGMHAITSPIAGLEGTEGSALNEGQADMWAFTITDNASLGDY
                     VVNAKGYRQLYRDRGRNPDSIAYIRSARSTLKYSDIATYKNGTAYEFEEHRDGEIYMS
                     TMWDIREMLNRVYPQTSYKRPRPIDGQALKVVTKGTEIFERDFLGSMYILGTTAPDSM
                     VKAKDAMIVADQMLYSVDASDPDAPGKHRSLIEQIFAAKELGVNALEVTGGKATISTQ
                     VTPFVGNQAAPEVPTNIQVAPTSPNSLKVSWNAVPGAVSYQVLKRKIGFENRREPNGK
                     REFADGDASTTGFRHVAFVNGNQSSFEDKGA"
BASE COUNT         1433 a         1784 c         1681 g         1351 t
ORIGIN      
        1 cgctccgatg tgcgcctgtg tccctacgcc gtcgcaaacc agcatctcgg aggtcatcga
       61 catcaaagcg ggcgtcgggc atacgctcgt ccgcaaacgc gacggcagcg tttgggcttg
      121 gggctcgaac gtcgtcggac aagcgggcaa cggaacaacc accgccgccc aattgcctgt
      181 gcagtcaaat gtcggaacag gcaatgtgtg gattgccgcg ggcttatact acagccacgc
      241 cggaattcct tcgattgcca cttttacggg ttcaaacgtg cgtctttacg gcgaaaacgt
      301 gaacatcacc ttcgccgacg tgaccgcgtc cggcacgacc cattattcgg caatcgaccc
      361 gaacgccttt gcgtcgcccg ccggtttcga catcgtggat aattctccgg cttacaacct
      421 gacgacgagc gcgtttttca cgtcggcaaa tgtctgcctg aaagttacga ccgagtattc
      481 gccgaccaga ttcgacgctt tgcgcctttt gcatcaggaa aacggcgttt ttgtcgaccg
      541 aaccgtttcc agagattatc gacggcggga agtttgcgcc ggcgtgacga gcttttcgcc
      601 gtttgtcctg gcgctgccgg tcgcttccgc atttgattcc gaaggcggca aagacgacta
      661 aaccttcaac gtcggaataa ctatctgcgc ccggcgcgcc gggcgcaaat ccggcgcggc
      721 agatttctcc gtatcccgaa tcaacacaat ctcaagtttg cctcaagtgc tctcaaaccc
      781 gcataaaaac tggctaataa atcaatctta aaacgcatcg gcggctcaag tatttctcaa
      841 agcccgcgcg gtatcttttc aacatacggt tttcaaattt tttgttgagg agaatgtgag
      901 atgaaattca gagtaagcca aacgctgcaa cgaagatttt tcggagtgat atttgttttc
      961 gcgctggtcg gcgtcctttt cggacagagc tttgccgccg ccaagctgag cccggcgctg
     1021 cgcacaaaat taaacggtct tgccaacagc gcgcaggtcg gtatggtcgt cgtggccttt
     1081 aagacgaaca acgggctgca aaattcgcat ttgaacattt tgcgaagcgt cggcgttctg
     1141 ggcggacaga cgtttccgac cctgggaatg gtcgcacagc cgatgaccgc cggacaggtt
     1201 cgcgcgctgc aaaacaattc ggcggtgcgt tccatctggt caaacgaccg gcttcattac
     1261 tatatgcatc aggcgcgcgt agtcggcggc gcggcgcgcg ttgaaaccga ccgcgctttt
     1321 gcgattcgca acggtggctt acccgtttcg ggcgcgggcg atttttcggt tctcgtcatc
     1381 gactccggca tcgacgcgac gcacgcggat ttgcagttcg gcacgaaagt catccagaac
     1441 gtgcagacgc cggtcgccgc cggaacgctg cccggcttta cgccgcacgt ttcgattgaa
     1501 aacgttccga acacagacca atcggtcggg cacggaacac actgcgccgg aattatcggc
     1561 ggcacgggcg ttcgctcggg cggtctttat ccgggcgttg cgccgggcgc aaaaatcatc
     1621 ggagcgggct tgggcgcggg aattttcgtg attaacggct tggcggcttg ggaatggggc
     1681 ttggcgaatc agtatcgcta caacattcgc gtcgtttcca attcctacgg cggcgacgga
     1741 ccgttcgacc ccgaagaccc gataacgatt gccagcaaaa tggcttacga ccgcaatata
     1801 accgtcgtgt tcgcgggcgg aaacgccggt ccggtgaaag atacttataa tcctctggca
     1861 aaagcgccct gggttatcgg cgttgcggca ggctcgaaag aaggcgattt ggcggatttc
     1921 tcctcgcgcg gaatgccgcg tgaacaacgc ttaagcaatt ccgacccgct cgacgataac
     1981 aacgcgccga cgattaccgc gccgggaatg ggacgcgctt tcgaaagcag cctgacgcga
     2041 gccggtttca cgattgatat gatttcgacg cgctcgacct cgaatctggt cgccaacggc
     2101 acgaccaacg acacggagat tgcgccggga atgatcccgt tctacacgca gattcacgga
     2161 acttcgatgg cgacgccgta cgtcgcgggc gtggtggcgc tgatgctcga cgccgacccg
     2221 aatttaacgc cggacgaaat caaagaaatc ctgacctcga ccgccagcaa aatgccgggt
     2281 cgcagcgaat gggaagtcgg cgcgggatat atcaatgctt atgccgccgt cgataaggtt
     2341 ttcaaccgca cgcgcaacta cgcgcatttt caggatgtca gcttcaacgc cgtcttcagc
     2401 gaacagcgac cggcgcagca gaattttcat ctcgatttca acccggcggt ttccggcgcg
     2461 acttcgacca acgcgacgac ttttacggtc gaagcgggaa tgaacgtgct ggacgttttc
     2521 gccaccgtcg acagcattgc cggaggcacg ggcaatctgg tcggcatccg catcaccagc
     2581 ccaagcggcg tcaattactc gaccgccatc gattatccgg ttatcggcac ggacaaacgc
     2641 cagattgtcg tccagaatcc ggaagccgga acgtggactc tggaagttcg cggcgcgcgc
     2701 ggtttgacgg cggctccgca ggcttcttcg ccgattcagt tggcaatgcc gggtccggtc
     2761 gacggcaatg tcacgcagat taaatacact ttgccgaata ttgcggacat tcagggacac
     2821 ccgcagcagg cggcaatcga agcggctttg aaaaaccgcg tggtcgacat ctacgcggac
     2881 ggcactttca aaccgaacga cacggtgacg cgcgaagatt ttgcccgctc gctcgttctg
     2941 aacacatcgc ttcgccagac gctgtcggca acgccgagat tcggcgacgt ttcgggcgac
     3001 ctcgccagaa tcgccgaagc cgtcacggca aatggctcaa cgctgcgcga tttcaactat
     3061 gttccggacg gaatgatgac cggagacgat aatttctacc cgaacgccgc ggtcactcgc
     3121 atcgacatgg caattgcttt tgtgcgtgct ctcggttacg acacgcaagc tcgcaatctg
     3181 gcaaattcgg acgtcacgtc taacggcgcg gtgatttccg acaacgggca gattcccgca
     3241 gaacttcgcg gctatgttca gattgctctg gataaaggct tgtttgaagc gtttccggcg
     3301 ggagtcgtcg aaatcgctcc cggacaattc caggttcttc cgggaccgcg cttcgagccg
     3361 aacacgaccg ttaaccgcgc aacgctcgca attaaactga acagctaccg ccagttgttc
     3421 acaactggcg gctaatcgaa aggcgaaagg cgaaggcgaa aggcgaaata aaaagtttta
     3481 agtgttgagc gttgaatttt gaacgaccag tcaacaacag actcaaaact caacacttaa
     3541 aactcgaaat tctcttttct ttatcgctca gcctttcgcc tttcaccttt ctcaagtctg
     3601 cctcaagtcg gaaggctcag cccattcaag cctttctcaa gtcgaaaaag ttatcgtgct
     3661 gacagttaca aaaattattt ttagtttcca ttggaggatg aaatgaaggg caaggtcaac
     3721 agaacaacag aaaaagcagg attcaagctt atcgcggtat ttttgttttg gtcatttatt
     3781 ataacggttc tttatattcc gttcggcact gccgaggcgt cgagcaatca agctccgggc
     3841 gcgaattcgg gcagcctgta ttatcgcccg gcgtcgaatt tcgacctgaa cctgacgagc
     3901 tcggttgccg gtctgcgcgc ggcgaccgtc gagcagctcg gcgctttgaa tactttgaaa
     3961 acttcgacta acgcgtcgaa tatgacggtt cgctggaacg ctttcggcgg ctcgccggac
     4021 gttgtttacg attttgccag ccagccgttt tcgggaacgc ccgaagaagc cggacgcgct
     4081 tttatacaac aaaacgcagc cgttttcggc gtcggcagtc ttaatgattt aaaacttttc
     4141 agccagcgcg aggcgctcgg cggttatctt ttgcgcttcg gtcagacttt taacgggatt
     4201 gacgtgaaag acggcggcat cggcatcgtg atgaatgcca ataagcaggt cattatggct
     4261 tccggtccgt tcttccgcga cgtcaatgtt aatacgacgc cttcgatttc cgccgaccag
     4321 gcgcgcgcga cggtcaattc ggacttggcg caatactcca acagtatcgt aagttccgcg
     4381 tcgaatctgt ttcagcctgc tttgaacgtc gtttcgcagc agacgcagat tgtcaacagc
     4441 atccaaccga agcttggcat ttacccgacc gccgacggtt acaagctggt ttggaaagtg
     4501 gcgaaaatgt caactaatcc gttcggcttg tacgcgtatt cgattgacgc ccatacgggc
     4561 gaaatcgtcg cccgcaaaga cttcgtgaaa ttccagcaga atccggttca tccgtttact
     4621 gccgacattt acccgaaata cccggacatt acgccggagc tgaaagacca gtcgattatt
     4681 aaagattgcc tgaacgaaca aaacgtgatg gctccgtgcg gacagcagcg agtcaagctt
     4741 cgttcttttg accagcagaa cgtcgtgacc ggcttgaacg gaactttgac cggcacgcac
     4801 gcgctcgtta acaacgtgct tgcgaccaaa cagcctttcg cgcaggcggc gctcggaacc
     4861 tggcacttcc gcaaagacga tccgaccgct tttgaaggac gcaccaacga gcaggaccaa
     4921 tatgccgagc ccgccgagca tcaggacgaa attaatgctt tctttttcgt cacttatctg
     4981 ctcgaatacg tcgattacct gcacgtcgcg ggcgacaaca cgaacggctt gcagggcaga
     5041 ttcccggatg aatatccgaa caagacgatt ccgcttccgg caaccgttca cttcccgaac
     5101 atttacgctg tgctgcccct gttgggctgc ccggcaaact gtcccgttcc cagtccaacc
     5161 gacccggatt tatcgaaaaa gcttctcggt ctggacaacg caatcgccgt caacgtaacc
     5221 agcttgattg aagaagtcac cgccgaaaaa tcgccggtcg tggttaaccc gactttctac
     5281 ggtcacggct atctgctgaa cgacctcgct ctcgaaggca ctgttcccta tcacgaagga
     5341 atgcacgcca tcaccagccc gattgccggt ctggaaggaa cggaaggctc ggcgctgaac
     5401 gaaggtcagg cggatatgtg ggcgttcacc attaccgaca acgcttctct gggcgattac
     5461 gtggtcaatg ccaaaggcta tcgtcaactt taccgcgacc gcggacgcaa tcccgactcg
     5521 attgcctata tccgctcggc tcgctcgact ctgaaatact cggacatcgc gacgtataaa
     5581 aacggcacgg cttacgaatt tgaagaacat cgcgacggcg aaatctatat gtcgacgatg
     5641 tgggacatcc gcgagatgct gaaccgcgtt tacccgcaga cgtcatataa acgcccgcgt
     5701 ccgattgacg gtcaggctct gaaagtcgtc acgaagggca cggaaatttt cgagcgcgac
     5761 ttcctcggtt cgatgtatat tctcggcacg accgcgccgg acagtatggt caaagccaaa
     5821 gacgcgatga ttgtcgccga ccagatgctt tactcggttg acgcgtcaga cccggatgcg
     5881 cccggcaagc atcgctccct gattgaacaa atctttgcag ctaaagaact cggcgtcaat
     5941 gcgctcgaag ttacaggcgg caaagcgacg atttcgacgc aggttacgcc gttcgtcggc
     6001 aaccaggcgg cgccggaagt tccgaccaat attcaagttg ctccgaccag cccgaactcg
     6061 ctgaaagttt catggaacgc ggttccggga gcagtttcgt atcaggttct gaaacgcaaa
     6121 atcggctttg aaaaccggcg tgagccaaac ggcaagcgcg aatttgcgga cggcgacgct
     6181 tcgactaccg gcttccgcca cgtcgctttc gtcaatggca accaatcttc atttgaagac
     6241 aaaggcgct
//
LOCUS       DASWNE010001007         2159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_104624, whole genome shotgun sequence.
ACCESSION   DASWNE010001007 DASWNE010000000
VERSION     DASWNE010001007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104624"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..872)
                     /locus_tag="VGB68_19390"
     CDS             complement(201..872)
                     /locus_tag="VGB68_19390"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY0051470.1"
                     /translation="MKTLLSIAILLLSFVFTASAQTGEQAPILEKDVQYKDWTYKSVR
                     DEKEINLRNLAAAKKLVMVVYYAPWCPNWRNEAPIAQKLYEKYKANGFEVVGVGEYDA
                     VEKMKANLEAMKITFPVVYESDSPAAKQKTPHYEYRKATGDTRNWGSPWNIFLESKKL
                     EKKGDILLGKAFIVNGELIEAEAEAFIRQKLGLPTEDKKDALTAEKTSEICTPEKKVA
                     EFKKP"
     gene            complement(1032..1520)
                     /locus_tag="VGB68_19395"
     CDS             complement(1032..1520)
                     /locus_tag="VGB68_19395"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HEY0051471.1"
                     /translation="MQKLIYKLHLWLGLFVSVPVLAWALSGFLYALPNAVEGGSVEKI
                     DASRVKIAPAEAMRKAGELAGKQLPTTALTLLMKDGRPQYQSIGGLGADSIFIDAETG
                     EAQMSEPPTWKTRFFREAHFYFFAGAWQVTLLTVFSLLASLSALTGIYLNIVYWFGRW
                     KS"
     gene            1654..2082
                     /locus_tag="VGB68_19400"
     CDS             1654..2082
                     /locus_tag="VGB68_19400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051472.1"
                     /translation="MSNQTTVSDIFRRALEIRQANPNGSYKDVKSQIVSEFGGKPFPS
                     TDRLTIPEYDNIAPEEDWTAGLPIVLRGIQLQDWSEVAHGIIISLEQVENYPKESGRE
                     DDPEKEWRNRHKGIAEAEEKNLSKWMPEELLAVAKRNVKN"
BASE COUNT          548 a          542 c          518 g          551 t
ORIGIN      
        1 ctccgatatg tcgggcatcg ccgaagacca ggcggtcggc ggtcagttta cggaatcggg
       61 caggacgctt ctggcggtcg tggaaaaatt gtcggatacg ctggctgcgc tgccttaagc
      121 aagcggaaag cggcaaacga gaagaggtaa gttgcaatta aaaagtattg caacttacct
      181 cttctcgttt agaactaatt ttaaggcttt ttaaattccg cgactttctt ttccggcgtg
      241 cagatttcag atgtcttttc ggcggtcaga gcgtcttttt tatcttcggt cggcagaccc
      301 agtttttgcc tgataaaggc ttcggcttcg gcttcaatca attcgccgtt gacgataaag
      361 gctttgccga gcagaatgtc gccttttttc tccagttttt tcgattcgag aaagatattc
      421 cacggcgagc cccagttgcg cgtgtcgccg gtcgctttgc gatattcgta atgcggggtt
      481 ttctgtttcg cggcgggcga atccgattcg taaacgacgg gaaaagtaat cttcatcgct
      541 tcgagattcg ctttcatctt ttcgaccgcg tcgtattcgc cgacgccgac gacttcgaag
      601 ccgtttgcct tgtatttttc gtaaagcttc tgcgcgatgg gcgcttcgtt gcgccagttc
      661 ggacaccacg gcgcatagta aacgaccatc accagctttt tggctgcggc aagattgcgc
      721 agatttattt ccttctcgtc gcgaaccgat ttataagtcc aatctttgta ttgaacgtcc
      781 ttttccagaa tcggcgcttg ctcgcccgtc tgtgccgaag ccgtaaacac aaaacttaaa
      841 agcagaattg cgatagataa aagcgttttc atttttttga taactccctg cggttagact
      901 aacttttcag atgccgaaag acaagtaaaa gttagcttta aactgtcgaa tctattacgc
      961 gcgcagttaa tggaaagttt aaaatgcaaa attaaatttt tcttccattt tccgtttcgc
     1021 gtttttccgc ctcagctttt ccatcttccg aaccagtaaa cgatattcaa ataaatgccg
     1081 gtcagcgccg aaaggctcgc cagcagcgaa aaaacggtta aaagcgtcac ctgccacgct
     1141 ccggcgaaaa aataaaaatg cgcctcgcga aaaaatctgg ttttccacgt cggcggctcg
     1201 gacatttgcg cttcgcccgt ttcggcgtcg ataaaaatcg aatccgcgcc cagcccgccg
     1261 attgattgat attgcggtct gccgtctttc atcagcaaag tcagcgccgt cgtcggcaat
     1321 tgttttcccg ccagctcgcc ggcttttcgc atcgcttccg cgggcgcgat tttcacgcgc
     1381 gaggcatcga ttttttccac gctgccgccc tcgaccgcgt tcggcaaagc atacaaaaaa
     1441 ccgctcaaag cccacgccag aacgggaacg gaaacgaaaa gcccgagcca cagatgtagt
     1501 ttgtaaataa gcttttgcat atttttgaaa ccacaggtta acacagataa aaggcaaacg
     1561 ggcggatttc aaacgggcgg ttaaaatccg cgcgcatctg tgataatctg tgatttcatt
     1621 tctttttgca gaattaggag attttctaaa acaatgagca atcaaacgac cgtatccgac
     1681 atttttcgtc gcgcgctgga aatcagacaa gcgaatccga acgggtctta caaggacgtc
     1741 aaatcgcaaa tcgtcagcga attcggcggc aagccgtttc cctcgaccga ccggctgacg
     1801 attcccgaat acgacaatat cgcgcccgaa gaagattgga ctgccggttt gccgattgtc
     1861 ctgcgcggaa ttcagctcca ggattggagc gaagtcgctc acggcattat catcagcctc
     1921 gaacaggtcg aaaattaccc gaaagagtcc gggcgcgagg acgaccccga aaaagaatgg
     1981 cgcaaccgcc acaaaggcat cgcggaagcc gaagaaaaaa atctgagcaa atggatgccg
     2041 gaagaattgc tggcggtcgc gaaaagaaac gtgaaaaact gatagccggt aactgatagt
     2101 tgatagtttt gtctttcact atcagttacc ggctatcagt tatcagttat catgaaaat
//
LOCUS       DASWNE010001008         1672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_492758, whole genome shotgun sequence.
ACCESSION   DASWNE010001008 DASWNE010000000
VERSION     DASWNE010001008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492758"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..1111
                     /locus_tag="VGB68_19405"
     CDS             104..1111
                     /locus_tag="VGB68_19405"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEY0051473.1"
                     /translation="MAATAKTETKGLTLIEAIRQGIWEEMERDESVFVIGEDIGAYGG
                     AFKLTEGFIERFGAERMIDTPISEAAIVGAACGAALMGMKPVAEFQFIDFISCGFDML
                     SNYAAKSRYRGCGAIPAVFRGPCGSGVRGGPFHSLNAEAFFMNTAGLKMVEPSTAYDA
                     KGLIKAAIRDPDPVLFFEHKKLYRLPRLREELPADDYIVEIGKARTAREGRDLSIITF
                     GAQVLNAQDAAETLEKEDGLQVEVIDLRTLAPLDKEAILATVKKTNRALVLHEASLTG
                     GIGGEISAIIAEEAFEWLDAPVIRVASIDAPVPFAPQMEDYYLPQVSEIIDAARRLAA
                     Y"
     gene            1127..1612
                     /locus_tag="VGB68_19410"
     CDS             1127..1612
                     /locus_tag="VGB68_19410"
                     /inference="COORDINATES: protein motif:HMM:NF042691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6585 family protein"
                     /protein_id="HEY0051474.1"
                     /translation="MRKDGNERNQSYRMSELQLGKLESVHGISPVFMQRAAFVAVLSF
                     IFFVAMFIAFSIRQNIGYFLLATAFLLVQLLTLFGWLRQRRTEFKIYENGFSYKKQSC
                     RWDELESMAVKTESRLGGGEKVDCEIRKTSGEKIVLNEALRGVDKIMERISAEIEKRS
                     I"
BASE COUNT          421 a          407 c          478 g          366 t
ORIGIN      
        1 gcggctgcaa aagagaaggc gctgaggcgc ggctgaaaat atccgccgga attgtaatat
       61 aattgagaaa ctcaaataaa tctatcaaat ttagtcgaca ataatggcag caacagcaaa
      121 aacggaaaca aagggcttaa ccctaatcga agcgattcgg caaggcatct gggaagaaat
      181 ggagcgcgac gaaagcgtct tcgtcatcgg cgaagacatc ggcgcatacg gcggcgcgtt
      241 taaattgacc gaaggcttta tcgagcgttt cggcgcggaa agaatgattg acacgccgat
      301 ttcggaagcg gcgattgtcg gcgcggcgtg cggcgcggct ttgatgggta tgaagccggt
      361 cgcggagttt cagtttatcg actttatctc gtgcggcttc gatatgctgt cgaattacgc
      421 ggcgaaatcg cgttatcgcg gctgcggcgc gattccggcg gtttttcgcg gtccgtgcgg
      481 ttcgggcgtg cgcggcggtc cgtttcattc gctcaacgcc gaagcgtttt ttatgaacac
      541 agccgggctg aaaatggtcg agccttcgac cgcatacgac gcgaaaggct taatcaaagc
      601 ggcaatccgc gaccccgacc cggttttgtt ttttgaacac aagaagcttt atcgcctgcc
      661 gcgcctgcgc gaagaattgc ccgcagacga ttacatcgtc gaaatcggca aagcgcgcac
      721 cgcgcgcgaa ggcagagatt tatcaatcat cactttcggc gcgcaggttt taaatgcaca
      781 ggacgcggcg gaaacgctgg aaaaagaaga cggattgcag gtcgaggtca tcgatttgcg
      841 cacgctggcg ccgctcgaca aagaagcgat tttagcgacg gtgaaaaaaa ccaatcgcgc
      901 gctggttttg cacgaagcga gcttgacggg cggaatcggc ggcgagattt ccgccatcat
      961 cgccgaagaa gccttcgaat ggctcgacgc gcccgtcatt cgcgtcgctt cgattgacgc
     1021 gcccgtgccg tttgcgccgc agatggaaga ttattatctg ccgcaggttt cggagattat
     1081 cgatgcggcg cggcgtctgg cggcttatta aagacgactg cggcgtttgc ggaaagacgg
     1141 aaacgaaaga aatcaaagtt atcgaatgtc cgagctgcaa ttaggaaaac tcgaaagcgt
     1201 tcacggcatc tcgcccgttt ttatgcagcg cgcggcgttt gtcgccgtgc tttccttcat
     1261 cttttttgtg gcgatgttta tcgctttttc gatccggcaa aacatcggtt atttcctgct
     1321 ggcgaccgct tttctgctcg tccagctgct gacgcttttc ggttggctca ggcagcggcg
     1381 cacggaattt aaaatttacg aaaacggttt ttcatacaaa aaacaatcct gccgctggga
     1441 cgagctcgaa tcgatggcgg tcaaaacgga aagccgtctg ggcggcggcg aaaaagtcga
     1501 ttgcgaaatc cgcaaaacaa gcggcgaaaa gattgtttta aacgaagcgc ttcgcggcgt
     1561 cgataaaatt atggagcgaa tcagcgccga aatcgagaaa agaagcattt gaaaaggcaa
     1621 gagataaaag gcaaaaggca aaatcagaac ccgttcttcc attttacctt tc
//
LOCUS       DASWNE010001009         5010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_598551, whole genome shotgun sequence.
ACCESSION   DASWNE010001009 DASWNE010000000
VERSION     DASWNE010001009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598551"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1970)
                     /locus_tag="VGB68_19415"
     CDS             complement(<1..1970)
                     /locus_tag="VGB68_19415"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY0051475.1"
                     /translation="MSSRHKSRFILLALFAVLAGFISVWAHEGEDHSGETKTTANATN
                     AAPVSIVTAERNIQNESGNFNLILKRSPGDPRAGEVEQFLVRITEKVEGGFGGSEGAA
                     AVEKAVVTASITGAGGAKIEENLPVTAEAGGNYRTAHDFDDSGDYKIVFNVTTEDKRS
                     FSADFPVTVSRAPVRSSFWIGLAVLSLLTLGSLGAVFYAAKRKGEGKVNYRSLAPFAL
                     AAILIFALGAVALAYFLPPRETRVAAAIPLITTTETAANAPATGTVLTVPKESQLLFG
                     IKTDPVTTRKITSGLKTTGTVRARPDARAVVVPPVAGRIVLRAGVSLGSAVARGEQIG
                     YVEQILDVSGQTELESQRLEVEAQQREVESKRLELRNTALQLQAQQADQRAKAQQART
                     QLAQAQRELRRSENLVEVGAAPRKRVEEAQTAVKVAEQEVASAEQQVTLLGNQIKQTQ
                     AGQTIFRSPRVNQPNRTFPLTAPVTGTISEIKATSGQQVESGAELLSIVNLSTVLIEA
                     QVFERDLPIVRDSTRASFTSAALGGEVYTIGTPDGDGRLVSIGQTVNEQTRTVPVIYE
                     VINPLQRLRDGMFIEVTVDTTGDRDVLAVPKAAVVNEQGQTFVFVFDGGENFEKRPVA
                     LGAEGADYYEIKSGLKEGDRVVTEGIYQLRST"
     gene            complement(1999..2640)
                     /locus_tag="VGB68_19420"
     CDS             complement(1999..2640)
                     /locus_tag="VGB68_19420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051476.1"
                     /translation="MKKFTFITFVILSFIIGLAAACSSAGSNTTTGAGATNTNQTTAA
                     NTANTNATNTNAHKTEEDIPAAVKTALPDAQQFTKQHKDIPANSIAEIEKETGAKITD
                     KDHHSYLAFATVGGKRTQVGAATVVKAAGKDIVIVYESKNGMPTIKEVRADGVPAEFL
                     KQFAGKGHDDKFQVGADVKAGNGVDEAAAKTIAQAVRVDSMTMQTLYGAAHSH"
     gene            complement(2645..4201)
                     /locus_tag="VGB68_19425"
     CDS             complement(2645..4201)
                     /locus_tag="VGB68_19425"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY0051477.1"
                     /translation="MLQNYKNSGKGRSFKGASVASAVIFVLIAAAAAFSQQTVPAPTP
                     PAVQQPTIKPASSEYITAGGLTVERLIELGGSRRADLQAARQRLAIAEGRLQQARLRP
                     NPTLDAEYGSPRFLGGEAESDLSVGVSQVFELGRKRSKRAAVAELELQQVRAEVLSLE
                     RQLAAEIRTAYTNAVAAARQLDVIERLIAADEEIVRVTEARLNEGDVAPLDVNLVKVE
                     LNRLKVQMIQTKSELETQTLQLKTLTATDVAESLFLAPQADRPPRLELGLVELTELAL
                     KERADLQAARIGERLGTARINLARANAVPNIAGSVKYSRSTGITDLPASAGGGLAIDK
                     DSELTFGVSIDIPVFNRNQGEIASATGEQVQATRQREFLETTIRRDVAVAYRKYRAAA
                     ESLVLYSTQILPRAEENLRSVQAAYGYGDFSIFEVVSEQRRLTENITGYNQTLRDYYA
                     ALAELETALGTSLPPAALSSVNDSVLPDKNLVPNQINREEFLKNIEKVELPKRSILSD
                     KDAKKQQEKE"
     gene            complement(4219..4887)
                     /locus_tag="VGB68_19430"
     CDS             complement(4219..4887)
                     /locus_tag="VGB68_19430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051478.1"
                     /translation="MSKPAVIKLLSLLVLFSVLLGAGNASEAFAHGGEDHGDGESKTT
                     ASGKGMVSHTARLGDFELMLKHPLLEPDAATSARLFVTDFKTNTPAGDISPEVEVESS
                     NGAVTQAAIEKTDAAGSYVVKIPALPEGAYLVRAKLTYKGETDTATFSGVEVKTQPTL
                     SAPEGEISWTRFALIAFVFALVLALFGGLVFFVWRSTASSETVESESNESKPLEIKKE
                     TVSA"
BASE COUNT          955 a         1471 c         1390 g         1194 t
ORIGIN      
        1 tgtgtcgagc gcagttgata aatgccttcg gtcacgaccc ggtcaccttc cttaagtcct
       61 gatttaatct cgtaataatc cgcgccttcc gcgccgagcg cgacgggacg cttttcaaaa
      121 ttctcgcccc cgtcgaaaac gaacacgaag gtttgtccct gttcatttac tactgccgct
      181 ttcggcactg ctaaaacgtc acggtcgccg gtcgtatcga ccgtgacttc aataaacatg
      241 ccgtcgcgca ggcgttgcaa tgggtttatg acctcgtaga taacggggac ggtgcgcgtc
      301 tgttcgttaa cggtctgccc gattgagacg agccttccgt cgccgtcggg cgtgccgatg
      361 gtataaactt cgccgcccaa tgcggcgctg gtaaaactcg ccctggtcga atctctgacg
      421 attggcaaat cccgctcaaa gacctgcgct tcaatcagca cggtcgaaag attgacgatt
      481 gacagaagct cggcgccgga ttccacctgt tgaccgctgg tcgccttgat ttcgctaatt
      541 gttcccgtca ccggcgcggt caggggaaat gttctgttcg gctggttgac gcgcgggcta
      601 cggaaaatag tctgaccggc ttgcgtttgt ttgatttgat ttcccagaag cgttacctgc
      661 tgctcggcgg aagcaacttc ctgttcggca actttcacgg cggtctgagc ttcctcgacg
      721 cgttttcgcg gcgcggcgcc gacttccacc aggttttccg agcgtcgcag ctcgcgctgc
      781 gcctgggcga gctgcgtgcg cgcctgttgg gctttcgcgc gctggtctgc ctgttgcgcc
      841 tggagctgca aagcggtgtt ccgcagttcc agccgttttg attcgacttc gcgctgctgc
      901 gcttcgacct ccaggcgctg cgattcaagc tcggtctgac cggaaacgtc caggatttgt
      961 tcgacatagc cgatttgctc gccgcgcgcg accgccgaac ctaaagaaac gcctgcgcgc
     1021 aaaacgattc tgcccgccac cggcggcacg acgaccgccc gcgcgtcggg acgcgcgcga
     1081 accgtgccgg tggtttttaa tccgctcgta atcttgcggg tggtgacggg gtcggtcttg
     1141 atcccgaata aaagctgcga ttctttcggc acggtcagaa cggtcccggt cgccggagcg
     1201 ttggcagccg tttcggtggt cgtgattaaa ggaatcgcgg cggcaacgcg tgtttcgcgc
     1261 ggcggcagaa aataagccag agcgaccgcg ccgagcgcga aaatcaagat tgccgcgagg
     1321 gcaaaaggcg cgaggcttcg gtaattgact ttgccttcgc cttttcgttt tgccgcatag
     1381 aaaaccgcgc cgagcgagcc taaagttaat aaactcaaaa ctgcaagccc gatccaaaag
     1441 gacgagcgca ccggcgcgcg cgaaaccgtg acgggaaaat cggcggaaaa tgagcgctta
     1501 tcttcggtcg tcacgttaaa aacgattttg taatcacccg aatcgtcaaa atcatgggca
     1561 gtccggtaat tgccgccggc ttccgccgtg acgggcagat tttcctcaat ttttgctccg
     1621 ccagctccgg taatgcttgc cgtcacaacc gctttttcca ctgccgcggc tccttcgctg
     1681 ccgccaaagc cgccttcgac tttttcggtg attcgcacga gaaactgctc gacttcgccg
     1741 gcgcgcgggt cgcccggcga acgtttcaga atgagattga aatttccgct ctcgttttga
     1801 atgttgcgct cagcggtgac aatcgacacg ggcgcggcgt tcgtagcatt agcggtcgtt
     1861 ttcgtctcgc ccgaatgatc ttccccttcg tgcgcccaga cggagataaa tcccgccaga
     1921 acggcgaaaa gcgcgagcaa aatgaatctg gatttgtgtc tgctgctcat ggtcgtcggt
     1981 tactttccgt tccggtgttt agtgcgaatg cgcggcgccg taaagcgtct gcatcgtcat
     2041 cgagtccacg cgcaccgcct gggcaatcgt ctttgccgcc gcctcgtcaa cgccgttgcc
     2101 ggctttgaca tcagccccga cctgaaactt gtcgtcgtgt ccttttccgg caaattgctt
     2161 cagaaactcc gccggcacgc cgtcggcgcg gacttccttg atagtcggca taccgttttt
     2221 gctttcgtaa acaatcacga tgtcttttcc ggcggctttt acgacggtcg ccgctccgac
     2281 ctgcgtgcgt tttccgccga cggtggcaaa cgcgagataa gaatgatggt ctttatcggt
     2341 tattttcgcg cccgtttcct tttcgatttc ggctatcgag tttgccggaa tatccttgtg
     2401 ctgtttggtg aactgctgcg cgtcgggcag cgccgttttg accgcagccg gaatgtcttc
     2461 ttccgtttta tgcgcgttcg tgtttgtcgc gttcgtgttt gccgtgttag ccgccgttgt
     2521 ctgattagtg ttggtcgccc ccgcccctgt agtcgtgttc gacccggcag acgagcaagc
     2581 tgccgccaag ccgataataa aacttaaaat tacaaatgtt ataaacgtaa attttttcat
     2641 ttccttattc cttttcctgc tgttttttag cgtctttgtc cgacaaaata cttctcttcg
     2701 gaagctcgac tttttcaata ttttttagaa actcttcgcg gttaatctgg ttcggaacca
     2761 agttcttatc gggcaaaacc gaatcgttga cagaagagag cgcagcgggc ggaaggcttg
     2821 tgccgagcgc cgtttccagc tcggcgagcg cggcgtaata atcgcgcagc gtctggttgt
     2881 agccggtgat gttttccgtc aagcggcgct gctcgctgac gacctcgaag atggagaaat
     2941 cgccgtagcc gtaagccgcc tgcacggagc gcaagttttc ctcggcgcgc ggcagaatct
     3001 gcgtcgaata caaaaccagg gactccgccg ccgcgcggta cttgcgatag gcgacggcga
     3061 cgtcgcgcct gatggtcgtt tccagaaact cgcgctggcg cgtcgcctga acctgttcgc
     3121 ccgtcgctga ggcgatttcg ccctgattgc gattgaaaac cggaatatcg attgatacgc
     3181 cgaaagtcag ctcgctgtct ttgtctatcg ccagtccgcc gccggcgctg gcgggcaaat
     3241 cggtgattcc cgtcgagcgc gagtatttga ccgaacccgc aatattcggg accgcatttg
     3301 cccgcgctaa attgattcgc gccgtgccga gccgctcgcc gatgcgcgcc gcctgcaaat
     3361 ccgcgcgctc tttcaaggca agctcggtca attcgacgag tcccagttcg agacgcggcg
     3421 ggcggtctgc ctgcggcgcg agaaagaggc tttcggcaac gtcggtggcg gtcagagttt
     3481 ttaactgcaa agtttgcgtt tcaagctcgc ttttcgtctg aatcatttgt actttcagac
     3541 ggttcagctc gactttgacc agattgacgt cgagcggggc gacatcgcct tcatttaacc
     3601 gcgcctccgt gacgcgaacg atttcctcgt cggcggcaat cagtctctct atcacgtcga
     3661 gctgccgggc ggcggctacc gcgttcgtat aagccgtgcg aatttccgcc gccagttgcc
     3721 gctctaaaga cagaacctcg gcgcgaacct gctgcaattc aagctcggcg accgccgcgc
     3781 gttttgagcg ttttctgccg agttcaaaaa cctgcgacac gccaaccgac aaatcgcttt
     3841 ccgcttctcc gccaagaaaa cgcggagagc cgtattcggc gtcgagcgtc gggttcggtc
     3901 taagtctcgc ctgctgaagt cgtccttcgg cgattgccag gcgctggcgc gccgcctgca
     3961 aatccgcgcg gcggctgccc ccgagttcaa tcaaccgttc gaccgtcaaa ccgccggcgg
     4021 tgatatattc cgaagaagcc ggcttaatgg tcggctgctg cacggcgggc ggggtcggag
     4081 ccggaaccgt ttgctgcgaa aatgcggcag cggcggcgat aagcacaaag attaccgccg
     4141 atgctaccga cgcccccttg aacgacctgc ccttgcctga atttttgtaa ttttgcagca
     4201 ttttcgattc ctgatttatc aagctgaaac cgtttccttt tttatttcga gcggcttgct
     4261 ttcattcgat tcgctttcaa ccgtttcgct cgacgcagta gaacgccaga caaaaaagac
     4321 cagcccgccg aaaagcgcca gaaccagcgc gaaaacgaac gcaatcagcg caaagcgcgt
     4381 ccaggaaatt tctccttccg gcgcggaaag cgtcggctgc gttttaactt ccactccgga
     4441 gaaggtcgct gtgtccgttt cgcctttata agtcaattta gcgcggacta agtatgcgcc
     4501 ttcgggcaaa gccggaattt ttacgacgta gcttccggcg gcgtcggtct tttcgattgc
     4561 ggcttgtgtc accgcgccgt ttgaggattc gacttcgact tcaggactga tatcgccggc
     4621 gggcgtgttg gtcttaaaat cggtcacgaa taaacgcgcc gaggttgccg cgtccggctc
     4681 cagcagcggg tgtttgagca tcagctcgaa atcgcccaag cgagccgtgt gcgagaccat
     4741 tcctttgccg ctggcggtcg ttttcgactc gccgtcgccg tggtcttcgc cgccgtgcgc
     4801 gaaagcttcg gacgcatttc cagccccgag caaaacggaa aacagaacga gcagcgacag
     4861 cagcttaata accgccggtt tactcattct ccgaattgtg aaaatgcttt tcataaacgc
     4921 ttctcccgaa acactaaatc tgcctgtacc gcacagaaac ttctcaggcg ggaatggcaa
     4981 agcgcgtgcc aatccgtatc ggcggcgttt
//
LOCUS       DASWNE010001010         1877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_138198, whole genome shotgun sequence.
ACCESSION   DASWNE010001010 DASWNE010000000
VERSION     DASWNE010001010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138198"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..870
                     /gene="lexA"
                     /locus_tag="VGB68_19435"
     CDS             253..870
                     /gene="lexA"
                     /locus_tag="VGB68_19435"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEY0051479.1"
                     /translation="MQPRTRRQREILDFITEFINERGHIPSYQQIARKFKIASKSAIA
                     KHIAAMEKQGILQRRREDGSFGLELRAENLVNEAVCEIDWLEPANAESFRENWEQEPI
                     FVPKFLLGYYEPERIRAFRVPNDAMLDEHICEGDIALIEKRSFARDGAVVVAQAENRV
                     VLKRFYRSGANIELQPANRNYQTIRLSADKVSIHGILHGLLRPFS"
     gene            1193..>1877
                     /locus_tag="VGB68_19440"
     CDS             1193..>1877
                     /locus_tag="VGB68_19440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEY0051480.1"
                     /translation="MSNNPTPNASYSVTLRVKIQNKPGKLGEITTAIGRVGGDIEAID
                     IVSVGKDFLIRDITVNASSEKHDEEIVNALTNIDGVEIVQTSDRTFLMHLGGKIEMVS
                     KVPLKTRADLSMAYTPGVARVCEAIARDPEKAYTLTIKKNTVAVVSDGTAVLGLGDIG
                     AEAAMPVMEGKCQLFKEFGGVDAVPICLNTKDPHEIVQTIKNIAVAFGGIHLEDISAP
                     RCFEIEDRLK"
BASE COUNT          470 a          478 c          495 g          434 t
ORIGIN      
        1 aagttttcta cacagtttat tttagggtag agtgtaattc cgacttggct tactgataag
       61 tgacaatttc agggaaaagt ttcgctttac gtttttgatt attgacaaat cggagcttca
      121 aaatacgccc ggcgggaatt attaatttct tagttatgta tcggcggcaa taaattttcg
      181 acagctattt cgttaaagaa aattcgaccg gatttgatag actttcgcgc agctattaat
      241 taatatttcc caatgcagcc acgcacccga cgccagcgcg agattctgga ttttatcacc
      301 gagtttatca acgagcgcgg gcatattccg tcttaccagc agattgcccg caaatttaaa
      361 atcgcctcga aatcggcgat tgccaagcat attgccgcga tggaaaagca gggcattttg
      421 cagcgccggc gcgaagacgg cagcttcggg ctggaattgc gcgcggaaaa tctcgtcaac
      481 gaagccgtct gcgaaatcga ttggctggag ccggcaaatg cggaaagctt cagggagaac
      541 tgggaacagg aaccgatttt cgtgccgaaa tttcttttgg gctattacga gccggaaaga
      601 atccgcgctt tcagagtgcc gaacgacgcg atgctcgacg agcacatctg cgagggcgac
      661 atcgcgctga ttgaaaaacg ctcgtttgcc cgcgacggcg cggtcgtcgt cgcgcaagcc
      721 gaaaaccgcg tcgtcctgaa gcgtttttat cgcagcggcg cgaacatcga gctgcaaccg
      781 gcgaatcgga attatcagac gattaggctc tcggcggata aagtttcgat tcacggcatt
      841 ctgcacggac tgctgcgccc gttcagctga aacttcccgg aattacgcct cgttacgatt
      901 cgcgaatacg tcgaaggcag gcgcaaaatt tatttcagcc cgtttttgta tctgctgatt
      961 tggcgcggca tctttatcgt gataaatcat ttcgtcggcg attcggcagc cgcgaaaaag
     1021 gaaatcgccg cttaaaaatc ggttggtcgg cgcgccgcgg gttttggcga cgatattttt
     1081 ctgcgcgctg ctcgcgcagg cgatgctcga aggcgcggtt tatcttttat tttaactcaa
     1141 aggcactcat tagagtgttg cgcgaacgca ttcaaaacgc taatcttttt gaatgtcgaa
     1201 taatccaact ccgaatgcca gctacagcgt cacgctgcgc gtcaaaattc agaacaagcc
     1261 cggaaaatta ggcgaaatca ccaccgccat cggacgcgtc ggcggcgata tcgaggcgat
     1321 tgacatcgtc agcgtcggca aggattttct catccgagac attaccgtca acgcatccag
     1381 cgaaaaacac gacgaggaaa tcgtcaacgc cctgacgaat atcgacggcg tggaaatcgt
     1441 acagacgtca gaccgcactt tcctgatgca tctcggcggc aaaatcgaga tggtttcgaa
     1501 agttccttta aagacgcgcg ccgatttgtc gatggcatac acgccgggcg tcgcccgcgt
     1561 ctgcgaggcg attgcgcgcg acccggaaaa agcttacacc ttaaccatca aaaaaaacac
     1621 ggtcgcggtc gtctccgacg gcacggcggt tttgggcttg ggcgacatcg gcgcggaggc
     1681 ggcaatgccc gtgatggaag gaaaatgtca gctttttaaa gaattcggcg gcgtcgatgc
     1741 ggtcccgatt tgcctgaaca cgaaagaccc gcacgaaatc gtccagacga ttaaaaatat
     1801 cgccgtcgct ttcggcggca tccatctcga agacatttcc gcgccgcgct gttttgaaat
     1861 cgaagaccgc ctgaaag
//
LOCUS       DASWNE010001011         1933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_317535, whole genome shotgun sequence.
ACCESSION   DASWNE010001011 DASWNE010000000
VERSION     DASWNE010001011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317535"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VGB68_19445"
     CDS             complement(<1..437)
                     /locus_tag="VGB68_19445"
                     /inference="COORDINATES: protein motif:HMM:NF018743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033922 - peptidoglycan
                     beta-N-acetylmuramidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exo-beta-N-acetylmuramidase NamZ
                     domain-containing protein"
                     /protein_id="HEY0051481.1"
                     /translation="MATLQRKAVKLGLEKVLDEELHLLKNLRIGLICNQASVDHQFRH
                     AADLFFENADINLTTLFGPQHGIRGDVQDNMIETLHTTDRETGLPVYSLYSETREPTA
                     EMLASVDALVFDLQDVGCRVYTFIYTMANAMKACAELDKKMIVL"
     gene            complement(444..1130)
                     /locus_tag="VGB68_19450"
     CDS             complement(444..1130)
                     /locus_tag="VGB68_19450"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEY0051482.1"
                     /translation="MRLEMDTRRFYLGRDYSEAIESLGAIPFHLSLIPNEEYIAEALK
                     NLDGVLLPGSDTDVDPIRFGEEPHPKLKKIIPEKEETDFLILAEAEKLRIPLLGICFG
                     MQALNVARGGSLIQDIEAQVPNALKHEQGTPLERGSHTIEIESESLLAKLSGNKTGIR
                     VNSHHHQSIRAVGENLKATAWAKDGVIECIEDTRADRFVLGVQWHPELSWKTDELSRN
                     IFETFIANCK"
     gene            1243..1785
                     /gene="hslV"
                     /locus_tag="VGB68_19455"
     CDS             1243..1785
                     /gene="hslV"
                     /locus_tag="VGB68_19455"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEY0051483.1"
                     /translation="MNSQNSIRSTTVLLVRRDGKTAMAGDGQVTLGETVIKGNARKVR
                     RIHNDKILVGFAGSTADAFSLLTRFETKLEQYHGQLERAAIELGKDWRTDKILRNLEA
                     LLIVADAGGAFLISGKGDVIASDEGLLSVGSGSMFALAAARALMKHTELSAREIAEEA
                     LKIAGEICIYTNSNIVLEEI"
BASE COUNT          472 a          496 c          458 g          507 t
ORIGIN      
        1 aggacgatca tttttttatc gagttcggca caagctttca tcgcattcgc catcgtataa
       61 ataaatgtgt aaacgcggca gccgacatcc tgcaaatcga aaaccaacgc atcgacgctc
      121 gccagcattt ccgcggtcgg ctcgcgagtt tcgctgtaga gcgaataaac cggcaaaccg
      181 gtttcccggt cggtcgtatg cagagtttca atcatattgt cctgcacgtc gccgcgaatg
      241 ccgtgctgcg gtccgaaaag cgtggttaaa tttatatccg cattttcaaa aaacaaatca
      301 gccgcgtgcc ggaattgatg gtcgaccgaa gcctgattac aaatcaaccc gatgcgtaaa
      361 ttttttaaaa gatgcagctc ttcgtctaaa actttttcca agccgagttt aacggctttg
      421 cgttgaagtg ttgccatagt taattacttg cagtttgcga taaaagtttc aaaaatattt
      481 cttgataatt cgtccgtttt ccaggaaagc tccggatgcc actggacgcc taaaacaaat
      541 ctgtcggcgc gcgtatcttc gatgcattca atcacgccgt cctttgccca cgccgtcgct
      601 tttaagtttt ccccgaccgc tctgattgac tgatgatgat gcgaattgac gcgaataccc
      661 gttttgtttc cggataactt cgccagcagg ctttcactct cgatttcaat cgtgtgcgag
      721 ccgcgctcga gcggcgtgcc ctgctcgtgc tttaaagcgt taggcacctg cgcttcaatg
      781 tcctgaatga ggctgccgcc gcgcgcgaca ttcagcgcct gcatcccgaa acaaattccc
      841 agcagcggaa ttctgagttt ttccgcttcc gccaaaatta aaaaatccgt ctcttccttt
      901 tccggaatga ttttttttaa tttcggatgc ggctcttcgc cgaaacgtat cgggtcgacg
      961 tccgtatcgc tgcccggcag taaaacgccg tccagatttt ttaaagcttc ggcaatatat
     1021 tcttcgttcg gaatgagact cagatgaaaa ggtatcgctc ccaaactctc gattgcttcg
     1081 ctgtaatcgc gtcccagata aaaacggcgg gtgtccatct ctaggcgcat tgtgattccg
     1141 attcgcggtt tgatcatacg acttggataa tgttcaacca atcgatgcgg gtctgtcaat
     1201 ttgagcattt gaaattaaac ctttatcatt gtttatttga tgatgaattc acaaaactcg
     1261 attcgttcga caaccgtcct ccttgtcagg cgcgacggaa aaaccgcgat ggctggcgac
     1321 gggcaggtca cgctcggcga aaccgtcatt aaaggcaatg cgcgcaaagt ccgccgcatc
     1381 cataacgata aaattctggt cggttttgcc ggctcgactg ccgatgcgtt ttcgctttta
     1441 acccgttttg aaacgaaact ggagcaatac cacgggcagt tggaacgcgc ggcgatcgag
     1501 ctcggaaagg attggcgcac agataaaatt ctgcgcaatc tggaagctct tttgattgtc
     1561 gccgatgcgg gcggcgcgtt tcttatttcc ggcaaaggcg atgttatagc ttccgacgaa
     1621 ggcttgcttt cggtcggctc gggcagtatg ttcgcgctcg ccgccgcgcg cgctttgatg
     1681 aaacataccg agctgtccgc cagagaaatt gccgaagaag cattaaaaat tgctggtgaa
     1741 atctgtatat acacaaattc aaacattgtt ttagaagaaa tttaaaatgc cgatatacct
     1801 gtccggtgaa accgtggaaa aaccgaaatt agacgattta acgccgcgcg agattgtcga
     1861 gcagctcgac aaatacgtcg tcggtcaaac gctcgccaaa cgcgccgtag cggtcgccct
     1921 gcgcaatcgc atc
//
LOCUS       DASWNE010001012         3226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_492990, whole genome shotgun sequence.
ACCESSION   DASWNE010001012 DASWNE010000000
VERSION     DASWNE010001012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492990"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..554
                     /locus_tag="VGB68_19460"
     CDS             144..554
                     /locus_tag="VGB68_19460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051484.1"
                     /translation="MKKTLLYRLFGFGKIPAQYRAALESEGVLLSDEGIKGTVTYRNF
                     RAPGRYSKWKRQWYSASIILTKARLLAFRHSSQIIDVPLADERFRRLQFSLEDESNLL
                     IAFDATLFHDDWSGTIEYRFKTPHARAFLDKLHR"
     gene            672..1163
                     /locus_tag="VGB68_19465"
     CDS             672..1163
                     /locus_tag="VGB68_19465"
                     /inference="COORDINATES: protein motif:HMM:NF027567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY0051485.1"
                     /translation="MEDKRQESIRKLNDLIGDIEVAMLTTIDGGSLRSRPMQTQDFEL
                     DGDLWFFTSSKTHKAEEIEKDNRVNVSYAEPGSNSYVSISGTAEIVRDRAKIDEYWND
                     ILKAWFPEGKDSPDLVLLKVAVEQAEYWDSPSSTIVQLAGFVKAMVTGERADGGENRK
                     IDL"
     gene            1336..>3226
                     /locus_tag="VGB68_19470"
     CDS             1336..>3226
                     /locus_tag="VGB68_19470"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY0051486.1"
                     /translation="MKKLFLALIVLSAFSISIAGQNYTIQQYLNIRSAVSPSLSPDGK
                     RMAYLTNVTGTAQIWMMDLPSGAPRQITNYEDNVGFVRWSPAGNGLVFGKARGGNENT
                     QLFRMSNDGSQIKELTADPKVRHNFGGWSKDGKRIFYTSNKRNPNYFDVYMMEFAKAG
                     DADLIETIIYKHEGSNSVAAVSDDGRKLVISRSGTELSLDNNLYLLDVETNKATLLTP
                     HTGASEFGDVDFLPDGNSIVLSANSDREFSSLAQIGNLRAEAENKMFYVEKNAEWDTE
                     GIEVSPRGDLMAYTVNREGFSELNLRKIETGGKPLISNLNFKSEIVNLPARGVLSGLT
                     FSSDGSKLAFTFSGSKYNTDIWLYDLKSKKLTQITRSDRAGIAQESFIEPQLIKYKTF
                     DGREIPAWYYRPPVGIISSKGLFQKIPVIVSVHGGPEGQERPGFNPLYQYYLSRGYAV
                     LATNVRGSTGYGKTFSHLDDVEKREDSVKDLAYAVEWLKTSGGADAKRIAVMGGSYGG
                     YMTMAAITLYPELWAAAVNTVGIVNWETFLKNTSGYRRRQREVEYGMLARDIEFLRRI
                     SPIARVDKIKTPLFVIHGKNDPRVPYTEAEQIVKDLKDRGAIVEYKLFDDEGHGISKL
                     KNRLEL"
BASE COUNT          859 a          798 c          830 g          739 t
ORIGIN      
        1 cacgaccaca cgagttaacg agccgattaa agatacggca atcaaattcg tcatcgcgcc
       61 gcaggcggct cggcggtcgg caatcaaatt cgccttgatt tgaataaaat cttataaaca
      121 ctctcttgat ttgggaattt ataatgaaaa aaacgcttct ttaccggctt ttcggattcg
      181 gaaaaatccc cgcgcaatac cgcgccgcgc tcgaaagcga aggcgttctg ctctcggacg
      241 agggcattaa aggcactgtc acatatcgaa atttccgcgc gcccggcaga tattccaaat
      301 ggaagcgcca atggtatagc gcttccatta ttctcacgaa ggcgcgcctg ctcgcctttc
      361 ggcattcaag ccaaatcatc gacgtcccgc tcgccgacga gcgtttccgc cgccttcaat
      421 tctcgctcga agacgaaagc aatttactaa tagccttcga cgcaacgctg tttcacgacg
      481 actggtcggg aacgatcgaa taccgcttca aaacgccgca cgcgcgggct tttctggata
      541 aactacaccg gtaagccgtc taaaaatttc aaaatatctc cctgttgaaa tttttttaaa
      601 ggaataaaaa ttgctcgaac gaaaccggtc tgtctgattt cagaacaaat ttaacgaaca
      661 agggaaaata aatggaagat aaaagacaag aatctatcag gaagctaaac gatttaatcg
      721 gggacatcga agtggcgatg ctgacgacga ttgacggcgg aagcctgcgc tcgcgcccga
      781 tgcagacgca ggactttgag cttgacggcg atttgtggtt ttttaccagt tcgaaaacgc
      841 acaaagccga ggaaatcgaa aaggataatc gcgtcaatgt ttcgtatgcc gagcccggct
      901 cgaactctta cgtttcaatt tccggcacgg cggaaatcgt cagggatagg gcgaagattg
      961 acgaatactg gaacgatatt ctcaaagcct ggtttccgga aggaaaagat tcgcccgatt
     1021 tggttttgct gaaagttgcg gtcgagcaag ccgaatactg ggattcgccc tcctcgacga
     1081 ttgtccagct tgccggattc gtcaaagcga tggtcacggg cgagcgcgcc gacggcggcg
     1141 agaatcggaa aattgatttg taaaatttta acgaaacccg ggaaacgtaa aagacagaag
     1201 cctgtggaaa tttatctttc gccttccgtc ttttttattt tgctctttgc attttcgaca
     1261 atctggataa atcgaagccg atagtggata ataaatagaa attcactatc cgtttttatt
     1321 ttcgaggaga aaaagatgaa aaagcttttc ctggcgctta ttgttttgag cgcgttttcg
     1381 atttcgattg ccgggcagaa ttacacgatt cagcaatatt tgaatattcg ctcggcagtc
     1441 tcgcccagcc tttcgcccga cggaaagcga atggcttatc tgacgaatgt gacgggaacg
     1501 gcacaaatct ggatgatgga tttgccgtcg ggcgcgccga ggcaaatcac aaattacgag
     1561 gataatgtcg gtttcgtccg atggtcgccc gccggcaacg gtctcgtttt cggcaaggcg
     1621 cgcggcggca atgaaaatac gcagcttttc cggatgtcga acgacggctc gcagattaag
     1681 gaactgaccg ccgacccgaa agtgcggcat aatttcggcg gctggtcgaa ggacggaaag
     1741 cgcatctttt acacgtcgaa taaacgtaac ccgaactatt tcgacgttta tatgatggaa
     1801 ttcgccaaag ccggcgacgc cgatttgatt gaaacaatca tttacaaaca cgagggcagc
     1861 aattccgtgg cggcagtttc cgacgacggc agaaagctcg tcatttcgcg ctccggcacg
     1921 gagctgagtc tggacaataa tttgtatctg ctggacgtcg aaacaaataa agcgacgctt
     1981 ttaacgccgc acacgggcgc gagcgaattc ggcgacgtcg attttctgcc cgacggcaat
     2041 tcaatcgttt tatcggcgaa cagcgaccgc gagttttcat cgctggcgca aatcggcaat
     2101 cttcgcgccg aagcggaaaa caagatgttc tatgtcgaga aaaacgccga atgggacacg
     2161 gaaggcatcg aggtttcgcc gcgcggcgat ttaatggctt acacggtcaa ccgcgaaggc
     2221 ttcagcgagc ttaatttaag aaaaatcgag acgggcggca aaccgcttat ttccaatctc
     2281 aactttaaaa gcgaaatcgt gaatttgccc gcgcgcggcg tcctctccgg tctgacgttt
     2341 tcaagcgacg gcagcaagct ggcttttact ttttccggct cgaaatacaa caccgacatc
     2401 tggctttacg atttgaagtc gaaaaaatta actcaaatca cgcgaagcga ccgcgccgga
     2461 atcgcgcagg aatcgtttat tgagccgcaa ttgattaaat acaaaacttt tgacgggcgc
     2521 gagattcccg cctggtatta tcgcccgccg gttggaatca tttcgagtaa aggtcttttt
     2581 caaaaaatcc ctgttatcgt cagcgttcac ggcggaccgg agggtcagga gcgaccgggc
     2641 tttaatccgc tttatcaata ttatctgtcg cgcggttacg cggttttagc gacgaacgtg
     2701 cgcggctcga ccggctacgg caaaaccttc tcgcatctgg acgacgtgga aaaacgcgaa
     2761 gattcggtga aggatttggc ttacgccgtc gaatggctga aaacgtcggg cggagccgac
     2821 gcgaaacgca tcgccgtgat gggcggcagt tacggcggtt atatgacgat ggcggcgatt
     2881 acgctttatc ccgaattatg ggcggcggca gtcaacacgg tcggcatcgt caactgggaa
     2941 acttttttga aaaacacttc cggctatcgc cgccgccagc gcgaggtcga atacggaatg
     3001 ctcgcgcgcg acatcgaatt tctgcgccgc atctcgccga ttgccagggt cgataaaatc
     3061 aaaacgccgc tctttgtcat tcacggcaaa aacgacccgc gcgtgcctta cacggaagcc
     3121 gagcaaatcg tcaaagattt aaaagacaga ggcgcaattg tggaatacaa actcttcgac
     3181 gatgaaggtc acggcatctc gaaactgaaa aaccggctgg agcttt
//
LOCUS       DASWNE010001013         2143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_527697, whole genome shotgun sequence.
ACCESSION   DASWNE010001013 DASWNE010000000
VERSION     DASWNE010001013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527697"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1921)
                     /locus_tag="VGB68_19475"
     CDS             complement(<1..1921)
                     /locus_tag="VGB68_19475"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0051487.1"
                     /translation="MLHAKLSAVFFVALFAVSVCAQDFQQKIRAALENRDYAAAIAEL
                     ENLRRTDKKIFELNNYDYLLARTAETGGDFAAAMANYQSVVSRNSVLREYALWHLSQV
                     ARASGNLMLERLYLNELLTTAPNSLLIQAAAARMARSSFESKDFDAAIRHLSAAGGQS
                     LAALTDNKSKTILAVVPSGEPSRENLVLIGQSYLQTGKSTPAREIFTKLITNSPNPAQ
                     PDDYALIAAKALDEMDSEKGGFGKTAPRLADLEHFRRAKIYHFNRDFAAARLHFTAIV
                     ERFPQSPNLPEALYQIGRGYALENSFNEAIKWFERVSAEFPEDAYARDGLSQAASAYA
                     RVNKPKEAITRYQRFIEKYAGEENSERAYLNIIDILRDTGEDTEAVKWAQKTREAFKG
                     ALPEALAVFAQARVHIAKNDWQSALNDLNELQNFSDLGGTRVPGASNKPEVSFLKGYA
                     LEQMQQYAEAIDVYLSIPDGRAEYYGWRATERLKILANDERAKPHIAQKLNQLATNID
                     GRNPEAQRKAAQGILRLNDSAELRARMLEILRRSYQTLPEYQKIPNPKLLEFGRREVL
                     QEARKAQNGSLHQALADELLFLGLYDEAAPELETALREKLSPNVSEKLKSLAGMQTNA
                     NNLSVFPADTAFTLAV"
BASE COUNT          448 a          597 c          597 g          501 t
ORIGIN      
        1 aaaccgccag cgtgaaagcc gtatccgccg gaaagacgga taaattattc gcgttcgtct
       61 gcattccggc aagcgatttg agcttttccg aaacgttcgg cgagagtttt tcgcgcagag
      121 cggtctccag ttccggcgcg gcttcgtcat acaaaccgag aaaaagcagc tcgtccgcca
      181 gagcttgatg aagcgaaccg ttttgagctt ttctcgcctc ctgcaaaact tccctgcgcc
      241 cgaattcgag cagtttcgga ttcggaattt tctgatattc gggcagcgtc tggtagctgc
      301 ggcgcaggat ttcgagcatc cgcgcgcgaa gctccgccga atcgttcaaa cgcaggatgc
      361 cctgcgcggc ttttctctgc gcttccgggt ttctgccgtc gatattggtc gccagttgat
      421 tcagcttttg cgcgatgtgc ggcttggcgc gctcgtcatt cgccagaatc tttaatcttt
      481 cggtcgcgcg ccagccgtaa tattcggcgc gcccgtcggg aatcgaaaga taaacgtcaa
      541 tcgcctcggc atactgctgc atctgttcga gcgcgtagcc tttcagaaag gaaacttccg
      601 gtttgttcga cgcgcccggc acgcgcgtgc cgcccaaatc ggaaaagttt tgcagctcgt
      661 tcaaatcatt taaagcgctc tgccaatcgt ttttggcgat gtgaacgcgc gcctgcgcga
      721 aaacggcgag cgcttccggc agcgcgccct taaacgcctc gcgcgttttc tgcgcccatt
      781 tcaccgcttc cgtgtcctcg cccgtgtcgc gcagaatgtc gatgatattc agataagcgc
      841 gttcgctgtt ttcttcgccc gcgtattttt ctataaaacg ctggtagcgc gtaatcgctt
      901 ccttcggctt gttgacgcgc gcgtaagcgc ttgccgcctg gctcagaccg tcgcgcgcgt
      961 aagcgtcttc gggaaactcg gcgctcacgc gctcgaacca ttttatcgcc tcgttaaaac
     1021 tgttttccag agcatagccg cgcccgattt gataaagcgc ttcgggcaga ttcggcgatt
     1081 gcggaaagcg ttcgacgatt gccgtaaaat gcaggcgcgc ggcggcgaaa tcgcggttga
     1141 aatggtaaat tttcgcgcgg cgaaaatgtt cgaggtcggc gaggcgcggc gcggtttttc
     1201 cgaaaccgcc tttttcgctg tccatttcgt ccagagcttt ggcggcaatc aaagcgtaat
     1261 cgtcgggctg cgccggattc ggcgaattcg tgataagttt ggtgaaaatt tcgcgagctg
     1321 gcgtcgattt gcctgtttgc agatagcttt gaccaatcaa aacgagattt tcgcggctcg
     1381 gctcgccgga cggaacgacc gctaatatcg tcttcgactt attatcggtg agagccgcga
     1441 gcgactgacc gccggcggct gaaagatgcc tgattgccgc gtcgaaatcc ttgctttcga
     1501 agctgcttcg cgccattcgc gccgccgccg cctgaatcaa taagctattc ggcgcggtcg
     1561 tcaaaagctc gttcagataa aggcgttcga gcattagatt gccgctggcg cgcgccacct
     1621 gcgacagatg ccagagcgcg tattcgcgca aaaccgagtt gcggctgacg accgattgat
     1681 aattcgccat cgccgccgca aaatcgccgc cggtttccgc cgtccgcgcc agcagataat
     1741 cgtaattgtt cagctcgaaa atttttttat ccgtgcggcg caggttttcg agctcggcaa
     1801 tcgccgccgc gtaatcgcgg ttttccagag cggcgcgaat tttttgttga aaatcctgag
     1861 cgcaaaccga aacggcaaaa agcgcgacga aaaaaacggc ggaaagtttg gcgtgtagca
     1921 tagaaatcaa acttattgta acctattgac tattaaaaaa cagcgatgac agcacaaatt
     1981 ttaagcggca gagagattgc cgaagccatc aaacgggaag ttgccgaaga ggtcgcgcag
     2041 ctcgacgcga aacacgggtt tagaccgcgt ctcgccgtcg tgcgtgtcgg cgaagacccg
     2101 gcgtcggcgg tctacgtcgg caataaagtc aagacttcca ggg
//
LOCUS       DASWNE010001014         2079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_703934, whole genome shotgun sequence.
ACCESSION   DASWNE010001014 DASWNE010000000
VERSION     DASWNE010001014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703934"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2079)
                     /locus_tag="VGB68_19480"
     CDS             complement(<1..>2079)
                     /locus_tag="VGB68_19480"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEY0051488.1"
                     /translation="VHSDAAPDALGTSVAFDGESILSGAASKDSTRGAAYVFAPDVFT
                     QQRLSTPSETSHFGHSVAVEGDTAIVGASTELRSDPQTGAWADGAAYIFVRSGANWVQ
                     QARLIPNDLPNGRQFGESVAISGNTVVVGAFTDRIGTNQSQGSAYVFVRSGASWTQQQ
                     KLTASDGAAVDWFGEKVAVHGDTIAVGAYRDDIGANQDQGSVYIFTRGGTNWTQAAKI
                     TAGDGAAFDSFGRSVALAGNTLIAGASGDDSAKGSAYVFTGSGSNWAFQQRLTANDGL
                     TEDEFGKSVSLSGETAIIGAFADDNGTINQQGSAYVFIRSGANWTQQQKLIAADGAAV
                     DFFGWSVSISGETAVVGTNFDDIAPNTNNGSAYVYVRNGTTWTQQSKLAAADAGNGDE
                     FGFAVAIDGANIIVGADEKNLDITSSAGAAYIFTGTGAPVVNNRTAFDYDGDGKADLS
                     VFRPSAASWYLSHSSNNAFIAVQFGANGDLIAPADFDGDRKTDISVFRPADGGWYRLN
                     SSNNTFSALQFGSNGDLPVPGDFDGDSKADICVYRPSAGSWYRINSTNNQFIAAQFGI
                     AEDKPLVGDFDGDGKSDLAVFRPSNGTWYRINSSSDTFSPNQFGTSEDKPVAADYDGD
                     GKTDLAVYRPSVGDWYIVNSSNSAFTGIHFGVTEDKPAPADFDGDGKADLVVFRPSTG
                     TWYLLRTTAG"
BASE COUNT          438 a          616 c          564 g          461 t
ORIGIN      
        1 aagcctgccg tcgttctcaa cagataccag gttcccgtag aaggtcggaa aacaaccaga
       61 tcagctttcc cgtcgccgtc gaaatcagcg ggcgcgggtt tgtcttccgt cacgccgaaa
      121 tgtattccgg taaacgccga attgctgctg ttgacgatgt accaatctcc gaccgacggg
      181 cgatagacgg ctaaatccgt tttgccgtcg ccgtcgtaat cagccgctac cggtttgtct
      241 tccgatgtgc cgaattgatt cggagagaag gtatcgctgc tgctgttgat tctgtaccac
      301 gttccgtttg aaggacgaaa cacggctaaa tctgatttgc cgtcgccgtc gaaatctccg
      361 accagaggct tgtcttcggc tattccgaat tgtgcggcga taaattgatt gttcgtgctg
      421 ttgattcgat accagcttcc ggcagacggt cgataaacac aaatatcggc ttttgaatcg
      481 ccgtcgaaat ctccgggaac gggtaaatct ccgttgctgc cgaattgcaa agcggaaaaa
      541 gtattgtttg aagaattcaa acgataccag ccgccgtccg ccggtcgaaa cacactgata
      601 tcggttttgc ggtcgccgtc gaaatcggcg ggagcgatta aatctccgtt ggctccgaat
      661 tgaacggcaa taaaagcgtt gtttgagctg tgtgataaat accagcttgc tgccgaagga
      721 cgaaacaccg ataaatcggc tttgccgtcg ccgtcgtaat caaatgccgt ccggttgttt
      781 acgaccggcg cgccggtacc ggtgaaaata taagcggcac cggctgacga tgttatgtcg
      841 agattcttct cgtccgcgcc gacgataatg ttcgcgccgt ctatggcaac ggcgaagccg
      901 aactcatcgc cgttgccggc atcggcagca gcgagtttcg actgctgcgt ccaggttgtc
      961 ccgttgcgga cgtaaacgta agccgaaccg ttattggtgt taggcgcgat gtcgtcaaaa
     1021 tttgtgccga cgaccgccgt ctcgccggaa atcgaaacgc tccagccgaa gaagtcgact
     1081 gccgcgccgt cggcggcaat cagtttctgc tgctgcgtcc agttcgcgcc cgaacggata
     1141 aaaacgtatg ccgagccttg ctggttaatc gtcccgttgt cgtcggcgaa agcaccgata
     1201 attgctgtct cgccggacag cgaaacgctc ttgccgaatt cgtcctcggt cagcccgtca
     1261 tttgcggtca gcctctgctg gaaagcccag ttcgagccgc tgccggtaaa gacgtaagcc
     1321 gaaccttttg ccgaatcgtc accgctcgcg cccgcaatca gcgtgttgcc cgccagcgct
     1381 acacttctgc cgaaagagtc gaaagccgcg ccgtcgccgg cagtgatttt agccgcctgt
     1441 gtccagttcg tcccgccgcg cgtgaaaata tagaccgaac cctggtcctg attcgcacca
     1501 atgtcgtcac gatacgcgcc gacggcgata gtgtcaccgt gaaccgctac tttttctcca
     1561 aaccagtcaa ccgccgcgcc atcgctcgcg gtcaattttt gctgctgcgt ccaactggct
     1621 cccgaacgca cgaaaacgta cgccgagccc tgcgattgat ttgtcccgat gcggtccgta
     1681 aaagcaccga cgacgaccgt gtttcccgaa atagcgacgc tttccccgaa ttgtcttccg
     1741 ttcggcaaat cattaggaat taaacgcgcc tgctgaaccc agtttgcgcc gctgcgaacg
     1801 aaaatatacg ccgcgccgtc agcccacgcg ccggtttgcg ggtcggaacg cagttccgtg
     1861 ctggcgccga cgattgccgt atcgccttcg acggcgacgc tgtgtccgaa gtggctcgtc
     1921 tcgctcggcg tcgataatct ttgctgcgtg aaaacatccg gcgcaaaaac gtaagccgcg
     1981 ccgcgagtcg agtctttcga cgccgcaccc gagagaatcg actcgccgtc aaaagcgacg
     2041 ctggttccca gcgcgtcggg cgcggcatcg ctgtgaacc
//
LOCUS       DASWNE010001015         3684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_527738, whole genome shotgun sequence.
ACCESSION   DASWNE010001015 DASWNE010000000
VERSION     DASWNE010001015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527738"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2106
                     /locus_tag="VGB68_19485"
     CDS             <1..2106
                     /locus_tag="VGB68_19485"
                     /inference="COORDINATES: protein motif:HMM:NF040143.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HEY0051489.1"
                     /translation="DDPKKAVYYPFAEFSPEWQAMKFALKNDTPCRFIDLPQSHQLII
                     DSIEPVKIEENLVEKRHIEPETIEKNGETEIPKSKVRRDPLQFAAEAAGFGDGEIWWE
                     NLIETRGKVEHEDLFDGILELMTALREQAENEASDAESERDELREAFMREEIRRAQSE
                     GFKNIAVVCGAWHAPRLANFSEYKEDRKKLSNLSKSKLAATWIPYTYNRLAAASGYGA
                     GISSPEFYHQIWENPQNVAANWLARVASLLREQDIDASSASVIEAVRLTESLAAMRGL
                     SAVGLNELFDAVRAVLCFGDDAPLRLIGEKLIIGERMGAVPAETPAVPLQGDLQATQK
                     RLRFMPEAAQKTIELDLRKNNDLERSQLLHRLNLLDINWGSAQKSGGKGTFKEAWRLA
                     WQPELEIALIEANVWGNTICSAAANKVRKIAAGAEKLSQLTEIVEQTLLADLPDAIDF
                     LMTQLEAKTAVSSDVPAMMDALPPLADVLRYGNVRRTDASVVERVVDGLVTRICIGLP
                     NAAAALDDEAAAQMFERIVAVDNAVTLLQNENYDARWRGVLGKLADAARLNGLVKGRA
                     VRLLFDKKQLSGEDAARRINLALSTAVEPESAAAWLDGFLRGSGLVLLHNEILLKILD
                     DWIKTLKEEVFVRILPLLRRTFSTFHAPERRQIGNKIKSGASAVNIQTADAEPGEIDS
                     ARAGLVLPLLAQLLGAETN"
     gene            2112..3251
                     /locus_tag="VGB68_19490"
     CDS             2112..3251
                     /locus_tag="VGB68_19490"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0051490.1"
                     /translation="MAEENQLKRWRLILGGDEADGIGINLETADLQIDKTLEALYDSE
                     RSGGLAGSAPNVSRWLGDIRTYFPASVVRVMQKDALNRLGLERMLLEPEMLEAVEPDI
                     HLVSTLISLNRIIPAKTKETARAVVRKVVAELERKLANPLRQAITGSLNRSIRNNRPR
                     HHEIDWHRTIRANLKHYQPAYKTIIPERRIGYGRKTQSLRDIILCVDQSGSMAGSVVY
                     SSVMAAVMASIKAVNTSLVVFDTAVVDLTEMLSDPVDVLFGTQLGGGTDINRALGYCQ
                     TLIRRPGDTIFVLVSDLYEGGDQSEMLKRAASIAASGVQVIALLALDDKGAPFFDHHV
                     AAKFAGFGFPAFACTPDLFPDLMAAAIEKRDLWQWASLNEIVVNK"
BASE COUNT          934 a          920 c         1010 g          820 t
ORIGIN      
        1 gacgacccga aaaaagccgt ctattacccg ttcgcggaat tttctcccga atggcaggca
       61 atgaaatttg cgctcaaaaa tgatacgccg tgccgtttta ttgatttgcc tcaatcgcac
      121 caattgataa tcgacagtat agagcccgta aagattgaag aaaatctcgt cgagaaaagg
      181 catatcgaac cggaaacaat tgaaaaaaat ggcgagaccg aaattccgaa atcaaaagtc
      241 cggcgcgacc ccttacagtt tgccgccgaa gccgccggtt tcggcgacgg agaaatatgg
      301 tgggaaaatt taatcgaaac gcgcggaaaa gtcgaacacg aagatttatt tgacggaatt
      361 ctggagctga tgaccgcttt gcgcgagcaa gcggaaaacg aagcgagcga cgccgaatcg
      421 gaacgcgacg agctgcgcga agcttttatg cgcgaagaaa ttcgccgcgc gcaaagcgaa
      481 ggcttcaaaa atattgcggt cgtctgcggc gcctggcacg cgccgcgact ggcaaatttt
      541 tccgaatata aagaagaccg gaaaaagctc tcgaatcttt cgaaatcgaa gcttgccgcg
      601 acctggattc cttacactta caaccgtctg gcggcggcaa gcggctacgg cgcgggaatc
      661 agctcgccgg aattttatca tcagatatgg gaaaatccgc aaaatgtcgc ggcgaattgg
      721 ctggcgcgcg tcgcctcgct tttacgcgag caggacatcg acgcttcatc ggcgagcgtg
      781 attgaagccg ttcgtttgac ggaaagtttg gcggcgatgc gcgggctttc cgcggtcggg
      841 ctaaacgaat tatttgacgc cgtgcgcgca gttttgtgtt tcggcgacga cgccccgctt
      901 cgtctgatcg gcgaaaaact gattatcggc gagcgaatgg gagcggttcc ggcggaaacg
      961 ccggctgtcc ctttgcaggg agatttgcag gcgacgcaaa aacgactgcg ttttatgccg
     1021 gaagccgccc aaaaaacaat cgagctcgat ttgcgaaaaa acaatgattt ggagcgcagc
     1081 caattgctgc accggcttaa tttgctggat ataaattggg gctccgcgca aaaatccggc
     1141 ggaaagggaa catttaaaga agcctggcgg ctggcgtggc agccggaact ggaaatagcc
     1201 ttaatcgaag ccaatgtctg gggaaatacg atttgctcag ccgccgctaa caaagtccgc
     1261 aaaattgccg ccggagcaga aaaactctcg caattgaccg aaatcgtcga gcagacttta
     1321 cttgccgatt tgccggacgc gattgatttt ctaatgacgc agcttgaggc gaaaacggcg
     1381 gtttcgagcg acgttccggc gatgatggat gcgctgccgc cgctcgccga cgttttgcgt
     1441 tacggcaatg tgcgcaggac cgatgcgagc gtcgtcgaac gcgtcgtcga tggattggtg
     1501 acaagaattt gtatcggttt gccgaacgcc gcagccgcgc tcgacgacga agccgcggcg
     1561 cagatgttcg aaagaatcgt cgcggtcgat aacgccgtca cgcttttgca aaacgaaaat
     1621 tacgatgccc ggtggcgcgg agttttgggc aaattagctg atgccgcgcg gctcaacggt
     1681 ttggtcaaag gtcgggcggt gcggctttta tttgataaaa aacaactttc cggcgaagac
     1741 gccgcccgcc gaatcaatct ggcgctttcg accgccgtcg agcctgaaag cgccgccgcc
     1801 tggctcgacg gttttttgcg cggaagcggt ctggttttac ttcataacga gattttgctg
     1861 aaaattctcg acgattggat aaaaactctg aaagaagagg ttttcgtccg gatactgccg
     1921 ctgctgcgac gcactttttc aacctttcac gcgccggaac gccgccaaat cggaaacaaa
     1981 atcaaaagcg gagcgtcagc cgtcaatatt cagacggctg acgccgaacc gggagaaata
     2041 gacagcgcgc gcgccgggct ggttttaccg ctgctcgcgc aattgctcgg agcggaaacg
     2101 aattaaattt tatggctgag gaaaatcaac taaaacgctg gcggctgatt ctgggcggcg
     2161 atgaagccga cggaatcgga atcaatctgg agacggcgga tttgcagatt gataaaactc
     2221 tcgaagcgct ttacgattcc gaacgctcgg gcggattagc cggctccgcg cccaatgttt
     2281 cgcgctggct cggcgatatt cgcacatatt ttcccgcttc ggtcgtccgc gtgatgcaga
     2341 aagatgcgct gaatcgcctc gggctggagc gaatgctgct cgagccggaa atgctcgaag
     2401 cggtcgagcc ggacattcat ctggtctcga cgctgatttc gctgaatcga attattccgg
     2461 cgaagacaaa ggaaaccgct cgcgcggtcg tccgtaaagt cgtcgccgag ctcgagcgca
     2521 agctcgccaa cccgctgcgg caggcgatta cgggcagtct gaaccgctca atcagaaata
     2581 accgtccgcg ccatcacgaa atcgactggc accggacgat tcgcgccaat ctcaaacatt
     2641 atcaacccgc gtacaaaacg attattcccg aaagacgcat cggctacggg cgcaaaacgc
     2701 aaagtctgcg cgacattatt ttatgcgtcg atcaaagcgg ctcgatggcg ggctcggtcg
     2761 tttattcgag tgtgatggcg gcggttatgg cttcaatcaa agcggtcaat acttcgctgg
     2821 tcgtttttga tacggcggtc gtcgatttaa ccgaaatgct ttccgacccg gtcgatgttc
     2881 ttttcgggac gcagctcggc ggcggaacgg acatcaatcg cgcgctcggc tactgtcaaa
     2941 cacttatccg ccgaccgggc gatactattt ttgtattggt cagcgattta tatgaaggcg
     3001 gcgatcagag cgaaatgctc aaacgcgcgg cttcgattgc cgcttcgggc gtgcaggtca
     3061 tcgcgcttct ggctttagac gataaaggcg cgccgttttt cgatcatcac gtcgccgcta
     3121 aattcgccgg tttcggcttt ccggcattcg cctgcacgcc cgatttattt cccgacctga
     3181 tggcggcggc gattgaaaag cgcgatttat ggcaatgggc gagtttaaat gaaatcgtag
     3241 taaataaata actctgagct ttgagccgaa aatactaaaa aatagaattt ttgacagtcg
     3301 gaaataccgg taacttagaa aataaaattg cgcaaaaagt aaaaaaagca ctgcccgcga
     3361 tgtgaaaatt tctgccggtg caattgagat tgaaatgatt gatagaccat tccgctggaa
     3421 tttaactcgt cgaaatcatc cgggaagtct gataagcagg aatggtttag aaatctagtg
     3481 gcggaattgc gctcctgaca taacctgccg gaatgctttt gttatttccg ggcgatgtcg
     3541 tctgccggca ctaaattttt ttaattatta ctcggcttta acaaaacccg cagtttttat
     3601 tcaaaaacaa acaataaacg caactcgcgg gcgataaaaa atcagcgttt tccggcttca
     3661 aaaaaagtgc ttgcttttct tttg
//
LOCUS       DASWNE010001016         2053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_344885, whole genome shotgun sequence.
ACCESSION   DASWNE010001016 DASWNE010000000
VERSION     DASWNE010001016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344885"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VGB68_19495"
     CDS             complement(<1..209)
                     /locus_tag="VGB68_19495"
                     /inference="COORDINATES: protein motif:HMM:NF013118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051491.1"
                     /translation="MIFKLTERDLKTRFGNFREILYYDGQAEAVALVTGDVEAQENVF
                     CRIHSSCISAHVFNSIECQCREEMES"
     gene            complement(401..1780)
                     /gene="hydA"
                     /locus_tag="VGB68_19500"
     CDS             complement(401..1780)
                     /gene="hydA"
                     /locus_tag="VGB68_19500"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEY0051492.1"
                     /translation="MKTLIKNGRIVTAVDDYKADILIEDETISIIGAKLEMEADVVVD
                     ASNKLVIPGGIDPHTHMELPFGGTQSSDDFFTGTRAAAFGGTTTIIDFAVQTKGDSMT
                     AGVDAWHAKAQGKCAIDYGFHLITTDFEDSNRAEMFRLMDEGITSFKLFMAYPGVFLA
                     DDATIFRAMSAAGERGGLICMHAENGIVINEIIKRALAQGRTAPKYHALTRPTIAEAE
                     GVHRAIAIAEMAESPVYIVHLSCTDALNQVREARDRGIPAFAETCPQYLFHSIEDYGE
                     DFEGAKYVMTPPLREKHNQAELWKGLKMDDLQVISTDHCPFCMKEQKELGRDDFSRIP
                     NGAPGVENRMSLIYNGGVVENRISLNRFVELTSTAAAKMFGLFPKKGTIAVGSDADIV
                     IFDTEKEHVFGVENEHMNVDYSSYEGWKVKGKVETVLSRGRVVIENGEHKGKAGDGQF
                     LKRGTCVNI"
BASE COUNT          460 a          544 c          478 g          571 t
ORIGIN      
        1 gattccattt cctcgcggca ttgacactcg atgctgttaa agacgtgtgc ggaaatacag
       61 ctcgaatgaa tccggcaaaa gacgttttcc tgcgcttcca catcgcccgt caccagcgcg
      121 acggcttccg cctgcccgtc gtaataaaga atctcgcgaa aatttccgaa tctggttttt
      181 aagtctcttt ccgtaagttt aaaaatcatc tcaattcagg ttaagaagct cgtcggtttt
      241 ctttgtccaa aagattagtc tttccagata ctcttcaaca gcttcatttg ttatatcatt
      301 gtatttagaa tgaaagtgtc cgtattgatt atagatatta ttctcgattc ggttattaat
      361 taagcgctcg accggcgaga gtttcgcatt cgcctcgcgg tcaaatattc acgcaagttc
      421 cgcgttttag aaattgcccg tcgcccgctt ttcctttgtg ctcgccgttt tcgatgacga
      481 cgcgcccgcg cgacagcacg gtctcgactt tgccttttac tttccagccc tcgtaggaag
      541 aataatcgac gttcatatgc tcgttttcga cgccgaaaac gtgttctttt tccgtatcga
      601 aaatcacgat gtcggcgtcg gaaccgacgg caatagtgcc ttttttcggg aacagaccga
      661 acattttcgc ggcggcggtc gaggtcaatt cgacaaaacg atttaagctg atgcggtttt
      721 cgacgactcc gccgttgtaa atcaaagaca tccggttttc gacgccgggc gcgccgttcg
      781 gaatgcgcga aaaatcgtct ctgccgagtt ccttctgctc tttcatacaa aacgggcaat
      841 ggtcggtgga aattacttga aggtcgtcca ttttcaagcc tttccacaat tccgcctgat
      901 tgtgtttttc gcgcagcggc ggcgtcatca catatttcgc gccttcgaaa tcttcgccgt
      961 aatcctcgat tgaatgaaaa agatactgcg ggcaggtttc ggcaaaagcg ggaatgccgc
     1021 ggtcgcgcgc ttcgcgcact tgattcagcg cgtcggtaca cgacaaatgc acgatgtaaa
     1081 cgggcgattc agccatttcg gcaatcgcaa tcgcgcggtg gacgccttcg gcttcggcaa
     1141 tcgtcgggcg cgtcagcgcg tgatatttcg gcgcggttcg cccttgggcg agcgcgcgtt
     1201 tgataatctc gttaatcacg attccgtttt cggcgtgcat acaaatcaag ccgccgcgct
     1261 cgcccgccgc gctcatcgcg cgaaaaatcg tcgcgtcgtc cgccagaaaa acgcccggat
     1321 aagccataaa taatttaaag ctcgtgatgc cttcgtccat caatctgaac atctcggcac
     1381 ggttcgagtc ttcaaaatcg gtcgtaatca ggtgaaaacc gtaatcgata gcgcattttc
     1441 cctgtgcttt cgcgtgccag gcatcgacgc ccgccgtcat cgaatcgcct ttcgtctgca
     1501 cggcaaaatc gataatcgtc gtcgtcccgc cgaacgcggc ggcgcgcgtc ccggtaaaaa
     1561 aatcgtccga cgactgcgtt ccgccaaacg gcaattccat atgcgtgtgc gggtcgatgc
     1621 cgcccggaat cactaattta tttgaagcgt caacgacgac gtcggcttcc atttccagct
     1681 ttgccccgat aatcgaaatg gtttcgtctt cgattaaaat atccgccttg taatcgtcaa
     1741 ccgccgtgac gatgcgcccg tttttgatta gtgttttcat aatgaattaa ctaggtttca
     1801 atattcgctg attgcgatta ttattttccc gaccatttat ttatatcttc gcaaaacctt
     1861 tatccaaagc tgcaacaagc tcgtcaacat ggtctttcgt cgaattgagc atcaagcccg
     1921 tccgaatcac gttgccgtaa agactgggtt tacagcagcg tgatttttct gcaatgcaaa
     1981 ccgcttactt tttgtcagtt tttgcagggg atttatcggt ttttgcagac gaagtgttgt
     2041 ctttatcgcg aag
//
LOCUS       DASWNE010001017         1820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_245685, whole genome shotgun sequence.
ACCESSION   DASWNE010001017 DASWNE010000000
VERSION     DASWNE010001017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245685"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..1071
                     /locus_tag="VGB68_19505"
     CDS             67..1071
                     /locus_tag="VGB68_19505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051493.1"
                     /translation="MQEIQKAKFEIGSAQAFAFAFLAFCALGAALVGSFPLQFSILTI
                     FLFAGVHNFMEFRYFVARMPLRWGKSKTFYAVGIGGVVVLASAYLSIYFLYDNWLWSL
                     TKVQAMVAVWNTFFVLWLGLLFYLRGRQKPKTDWSPAFAVAFLLAALAWLVPNYWSLA
                     LVYLHPFIALWFVERQIRRTRREWLRAYHFCLATIPFFLIVLWLALGNQTHLPEDTSL
                     FSRISQHAGSEILPNVSTHLLVAAHVFLETIHYAAWILLIPLVDKRAVPWKLKEIPLA
                     ANKQGFPKLVSAVLIVSLGLVFALWLGFSIDYATTRDVYFAFAIAHVLAEFPFLVKML
                     "
     gene            1368..>1820
                     /locus_tag="VGB68_19510"
     CDS             1368..>1820
                     /locus_tag="VGB68_19510"
                     /inference="COORDINATES: protein motif:HMM:NF020076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HEY0051494.1"
                     /translation="MRKVFFSIVFCLLLTLGVQAQQVTQGGLEAVGQDGKSLGACPLK
                     HTDVKAEISGFLSRVKVTQEFENSFSDKIEAVYVFPLPQNAAVDDMTMRIGERVVRGR
                     IMKREEAREVYEAAKSNGQIASLLDQERPNIFTQSIANILPGEKVIIEI"
BASE COUNT          389 a          438 c          469 g          524 t
ORIGIN      
        1 aactgagaac tgatagtttt ttcttccgct atcaattatc agttctcagt tctcacctat
       61 cttcgtatgc aggaaattca gaaagcaaaa tttgaaatcg gctcggcgca ggctttcgct
      121 ttcgcgtttc tggcgttttg cgctctgggc gcggcgctcg tcggcagctt tccgttgcag
      181 ttttccattc tcacgatttt tcttttcgcg ggcgtccata attttatgga gtttcgctat
      241 tttgtcgcgc ggatgccgct gcgctgggga aaatcgaaaa ctttctacgc ggtcggcatc
      301 ggcggcgtcg ttgttttggc ttccgcttat ctgtcgattt attttctgta tgacaactgg
      361 ctttggagcc tgacgaaggt gcaggcgatg gtcgccgtct ggaacacgtt ttttgttttg
      421 tggctcggac ttctttttta tctgcgcggg cggcaaaagc cgaaaaccga ttggagcccg
      481 gcgtttgccg tcgccttttt gctggcggcg ctggcgtggc tcgtgccgaa ttactggagc
      541 ctggcgctcg tctatctgca cccgttcatc gcgctctggt ttgtggaaag acaaatccgt
      601 cgcacgcgca gggaatggct cagagcctat catttctgtc tggcgacgat tccgtttttt
      661 ttaatcgttt tgtggctggc gctcggaaat cagacgcatc tgccggaaga cacttcgctt
      721 ttttcgcgca tctcgcaaca cgccggaagc gagattttac cgaacgtttc cacgcatctg
      781 ctggtcgcgg cgcacgtttt tctcgaaacg attcattacg ccgcgtggat tctgctgatt
      841 ccgctggtcg ataaacgcgc cgtgccgtgg aaattaaaag aaattccgct ggcggcaaac
      901 aaacagggct ttccgaagct ggtttcggca gttttaatcg tcagcctcgg tctggttttc
      961 gcgttgtggc tcggtttttc gattgattac gcgacgacca gagatgttta cttcgccttc
     1021 gccatcgcgc acgtgctggc tgagtttccg tttctggtta agatgcttta aaattttttc
     1081 accgcagaga cgcagagaag aaattaacag ggatgaaggg gatagacagg gattaaagac
     1141 gatttaatta tccctttcat ccccttcatc cctgttaatt tctttaaaaa atctcttgtc
     1201 tccgcgtggc tccggtgaat ttttttgcaa gactttcaaa ccgctcgcgt tcccaaagcg
     1261 tgacgataaa agttttccgt tcgagcaact gctcgcgtgt ctcaaactac caatggattg
     1321 aaacactttc gggaacaaaa tttacaaaat ccattcgagg tgaagttatg cgaaaagttt
     1381 tcttttcaat cgttttttgt ttgcttttaa ctcttggcgt tcaggctcag caagtgacgc
     1441 agggcgggct ggaagccgtc gggcaagacg gaaaatctct cggcgcgtgt cctttgaaac
     1501 atactgacgt gaaagccgaa atcagcggtt ttctttcgcg cgtcaaggtc acgcaggaat
     1561 ttgaaaatag tttcagcgat aaaatcgaag ccgtttatgt ttttccgctg ccgcaaaacg
     1621 cggcggttga cgatatgacg atgcgcatcg gcgagcgcgt cgtgcgcggg cggattatga
     1681 agcgcgagga agccagagag gtttacgaag cggcgaaatc gaacggtcag atagcgagtt
     1741 tattagacca ggaacgcccg aatatcttca cccaatcaat cgccaatatt ctgcccggcg
     1801 aaaaagttat tatcgaaatc
//
LOCUS       DASWNE010001018         3303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_527845, whole genome shotgun sequence.
ACCESSION   DASWNE010001018 DASWNE010000000
VERSION     DASWNE010001018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527845"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1347
                     /locus_tag="VGB68_19515"
     CDS             <1..1347
                     /locus_tag="VGB68_19515"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY0051495.1"
                     /translation="GLSIFNQRSGNFEWNAGPIFSNIVLADEINRATPKTQSALLEAM
                     AEEQVTVEGVSRPLPLPFMVIATQNPTEHHGTYPLPESQLDRFMMRLHIGYPRLEDER
                     KILRDREMRNPLEFVRAVMSEREVSEPPTPTPGFSRVEKLKHLQTEIERVIRGKSETV
                     KLALVALLARGHLLIEDVPGIGKTTLSNALARALDLSFQRVQFTSDLLPSDVIGLSIF
                     NQRNGNFEFKAGPIFANIVLADEINRATPKTQSALLEAMAEGQVTVEGVSRALPAPFM
                     VIATQNPTEHHGTYPLPESQLDRFMLRLHVGYPSRDEERRILRDRERKNPLDYVQSVM
                     SEAEVLNLQETAAEVRVDEVLIDYLLRIVEQTRHSETVELGISPRGTLALFRSAQALA
                     LIDGRDFCIADDVKRLVVPCFAHRLIVSSRYSNVTRRTHEAEQILQEILLKTKVPI"
     gene            1452..2894
                     /locus_tag="VGB68_19520"
     CDS             1452..2894
                     /locus_tag="VGB68_19520"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY0051496.1"
                     /translation="MNLKILSQLFSFRDIRNAALGFGVVIGGLGLAGLTLWAHQSGNT
                     RLAGIAAGVSLIFVLLIVVFVVPPLARSASAEASQMDLPFEFTVGGAVFLGLLVIVGF
                     AAWNTGNNLLFLVLSFLVSALVVGFFIGNFCLKKLDVKMRFPETIYAEQATPILVSLY
                     NRKRLFPTISIVTEVRGREREKSLIADELSKILPAALVGKITRPPLLKHTLDYFVHVP
                     RRGFVENRAEHVFEHRGRFIIRDFELSTRFPFGFFRHRRRLSAQEAEIVIFPKVEPVQ
                     AEILDFPLEVGKLVAAKRGTGQDLLALRDYQPQDDLRHVDWKATARTRRLIVREFAAE
                     DDKRVTVIFDTRIRPSTKDKTKTLRQRIEEEQKGKRLSPASKRFEKGVSQTASLLNHF
                     NEEQTEIRLIIDDKIGEFGIGKTHLNDALKQLALIEPTFIENGEREFPAETVEEIFTA
                     RKNSYTFFVTTVKEEKLPAEIQQTAKVLRY"
     gene            3099..>3303
                     /locus_tag="VGB68_19525"
     CDS             3099..>3303
                     /locus_tag="VGB68_19525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051497.1"
                     /translation="MYLAGKDESGFSVFERERDCDWASAKISFLYESGYAETDELLKQ
                     LTAQSTGDSMANVEVEVVGKLRDE"
BASE COUNT          752 a          867 c          894 g          790 t
ORIGIN      
        1 gggctttcta ttttcaatca gcgcagcgga aattttgaat ggaacgccgg accgattttc
       61 tcgaacatcg ttttagccga cgaaatcaac cgcgcgacgc cgaaaactca atcggcgctg
      121 ctcgaagcga tggcggaaga acaggtcacg gtcgaaggcg tttcgcgccc gctgccgctg
      181 ccgtttatgg ttattgcgac gcagaatccg accgagcatc acggcacata cccgctgccc
      241 gaatctcagc tcgaccggtt tatgatgcgt ctccatatcg gttatccgcg ccttgaagac
      301 gagcggaaaa tcctgcgcga ccgcgagatg cgcaatccgc tcgaattcgt ccgcgccgtg
      361 atgagcgagc gggaagtcag cgaaccgcca acgccgacgc ccggtttcag ccgcgtcgag
      421 aaattaaaac atctgcaaac ggagattgaa cgagttatcc gcggtaaatc ggaaaccgtc
      481 aagctcgcgc tggtcgcttt actggcgcgc gggcatcttc ttatcgaaga cgttccgggc
      541 atcggcaaaa ccacgctttc caatgctctg gcgcgcgcgc tggatttgtc gtttcagcgc
      601 gtgcagttta cctccgacct tttgccgtcg gacgttatcg ggctttcgat tttcaaccag
      661 cgcaacggca attttgaatt taaagccgga ccgattttcg ccaatatcgt gctggcggac
      721 gaaatcaatc gcgccacgcc gaaaactcaa tcggctttgc tcgaagcgat ggcggaaggt
      781 caggtcacgg tcgaaggcgt ctcgcgcgcg ctgcccgcgc cgtttatggt tatcgccacg
      841 cagaacccga ccgagcatca cggaacgtat cctttgcccg aatcgcagct cgaccgcttt
      901 atgctgcgcc tgcacgtcgg ttatccgagc cgcgacgaag agcgccgcat cctgcgcgac
      961 cgcgagcgta aaaatccgct cgattacgtg cagtcggtga tgagcgaagc cgaagtttta
     1021 aatttgcagg aaacggcggc tgaagtgcgc gtcgacgagg ttttgatcga ttatcttttg
     1081 cgaatcgtcg agcagacgcg gcatagcgaa accgtcgagc tcggcatcag cccgcgcgga
     1141 acgctcgcgc ttttccgctc ggcgcaggct ctggctttga ttgacgggcg cgatttctgt
     1201 atcgccgacg acgtcaagcg tttggtcgtg ccgtgtttcg cgcatcgcct tatcgtcagc
     1261 tcgcgctaca gcaacgtgac gcgccgaacg cacgaagccg agcagatttt gcaggaaatt
     1321 ctgctgaaaa cgaaagtccc gatttagcga atcgcgaatt gtaaatcgaa aatcgtgaat
     1381 cgtaaataga atgtttgaat ctgaagatta aaccgattta cgattcacga ttttcgattt
     1441 acgaacaagc aatgaatcta aaaattctca gccagctttt cagttttcgc gatattcgca
     1501 acgccgcgct cggtttcggc gtcgttatcg gcgggctcgg acttgccggg ttgacgctct
     1561 gggcgcatca atcgggaaat acgcggctcg ccggaatcgc cgcgggcgtt tcgctgattt
     1621 ttgttctgct gattgtggtt ttcgtcgtgc cgccgctggc tcgaagcgcc agcgccgaag
     1681 cttcgcagat ggatttgccg ttcgaattta cggtcggcgg cgcggttttt ttagggcttc
     1741 tcgtcattgt cggtttcgcc gcgtggaata cgggcaataa tctgcttttt ctcgtgcttt
     1801 cgtttctggt ctcggcgctg gtcgtcggct ttttcatagg caatttctgt ctgaaaaaac
     1861 tggacgtgaa aatgcgtttt cccgaaacga tttacgccga gcaggcgacg ccgattctcg
     1921 tcagcctcta taatcgaaaa cgcctgtttc cgaccatttc catcgttacg gaggtgcgcg
     1981 gcagggaacg cgaaaaatct ttaatcgccg atgaattgag caaaattctg cccgccgcgc
     2041 tggtcggaaa aatcacgcga ccgccgcttc tgaaacacac gctcgattat tttgtacacg
     2101 ttccgcgccg cggatttgtc gaaaaccgcg ccgagcacgt tttcgagcat cgcggacgtt
     2161 tcattatcag ggatttcgag ctttcgacgc gctttccgtt cgggtttttt cgacatcgcc
     2221 gccggctttc ggcgcaggaa gccgaaatcg tgattttccc gaaagtcgag ccggtgcaag
     2281 ctgaaattct ggattttccg ctcgaagtcg gaaagctcgt cgccgccaag cgcggaaccg
     2341 gtcaggattt gctcgcgctg cgcgattacc agccgcagga cgatttgcgt cacgtcgatt
     2401 ggaaggcgac ggcgcggacg cggcgcttga ttgtccgcga gtttgccgcc gaagacgata
     2461 aacgcgtgac cgtgattttc gacacgcgca ttcgtccttc gacaaaagac aaaaccaaaa
     2521 ctttgcgcca gcgaatcgag gaagaacaga agggaaaacg cctctcgccc gcctccaaac
     2581 ggtttgaaaa aggcgtcagc cagacggcgt cgctgctcaa tcattttaac gaggaacaaa
     2641 ccgagattcg cctgattatc gacgataaaa tcggcgagtt cggcatcggc aaaacgcatc
     2701 tgaacgacgc tctaaagcag ctcgctttga ttgagccgac ttttatcgaa aacggcgagc
     2761 gggaatttcc cgccgagacc gtcgaggaaa tctttaccgc caggaaaaat agttatacat
     2821 ttttcgtgac gaccgtaaaa gaagaaaaat tgcccgccga gattcagcag acggcaaagg
     2881 ttttgaggta ttagattatg agaaaaacta caggtgtttt agctgcgctt tttacttttc
     2941 ttctaggcat ttcggtttat tttgttttcc tgaattttgc ggttgataaa tcaaagccgc
     3001 ttgtaaagcc tctcgaatat tcttcacttc cacttatgtc tctttgtgat gcgacaagta
     3061 agccggagtt ttatgacaac cgcgatattc gcgttagagt gtatttagcc ggaaaagacg
     3121 aatcgggatt ttccgttttt gagcgcgagc gggattgtga ttgggcaagc gcgaaaatat
     3181 catttttgta tgaaagcgga tatgcggaaa cggacgagct tttaaaacaa ctgactgccc
     3241 aatccaccgg agactcgatg gcaaacgtcg aagtcgaggt cgttggaaaa ctgcgggatg
     3301 aat
//
LOCUS       DASWNE010001019         6320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_33890, whole genome shotgun sequence.
ACCESSION   DASWNE010001019 DASWNE010000000
VERSION     DASWNE010001019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6320
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33890"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1630)
                     /locus_tag="VGB68_19530"
     CDS             complement(<1..1630)
                     /locus_tag="VGB68_19530"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY0051498.1"
                     /translation="MFYRLLKLFAFICCVLPFAANAQTTGKSYSSEIKRPATSAPQFP
                     SPVVFTDITARTGIDFKHNASPTSVKYLLETMGAGVGLIDFDNDGRLDVFFTNGAQLS
                     DPMPKGKTPEKTNAKFWNRLYRQRADGAFEDVTEKAGLKGVGYGFGVAVGDYDKDGFS
                     DLFVTHYGGACLYRNNGNGTFTDATKKSGVEVEGWAASAGFFDFDKDGRLDLFVTRYV
                     VWDFENGALFCGDARAGYRSYCHPDNFKPTTSLLFRQKADGTFENVSEKAGIAQSRGK
                     SLGVAFADFDDDGWTDVFVANDNTEQHLFRNKGDGSFEDTALIAGVAFDENGKRFAGM
                     GVDAADYDGDGRQDVAITAFSGETYPLYRNLGDWSFDYVTRSSGVAQITQLGTGWGIK
                     FIDADSDGRRDLFVVQSHVSDVIEKTTDFLKYKQPSLLMRNAGKEFQNVSLAAGEIFK
                     IDLAARGMASGDLDNDGDTDVIIAQTNAAPVILKNNGTKNHWLGIDLRGEKSAPGGEG
                     ARLTVTDANGRKQIFDASNSGSYLAANDSRILIGL"
     gene            complement(1630..2628)
                     /locus_tag="VGB68_19535"
     CDS             complement(1630..2628)
                     /locus_tag="VGB68_19535"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0051499.1"
                     /translation="MEQMQSVHDATASAQQERTMRERFGSFVQQQGALVLLVLVCVIA
                     TARYEAFLTPENLLNVLRQNSMLGIIALGMTFVILTGGIDLSVGSLLAVAGVIAANLA
                     DRGLVVALVAAVAATTVLGLINGTVIAKARIQPFIVTLGMMIAARGIALAVTSEQNVR
                     IPATATEFSWLGQGMIGFLPVPIVILIIVFAVGWLILNYTRFGRHVFALGDNDEAARL
                     MGLNIDRVTILVYTISGLLAGLAGVILASRLYTGSPTEGVGRELDAIAAVVVGGTLLT
                     GGKGSVVSTLYGVLLLGVIFNLFNLEGQISPWWQLVFRGAILLAVVILQNRLTRLG"
     gene            complement(2635..4245)
                     /locus_tag="VGB68_19540"
     CDS             complement(2635..4245)
                     /locus_tag="VGB68_19540"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEY0051500.1"
                     /translation="MKPQINADESRLNQKISASICVYLWLAFLFVLPSQAQTYVNPVI
                     AGDFPDPSVIRVGEDYYATATTGGWSPHFPLLHSKDLINWEIIGAVFGEKPAWAKGDF
                     WAPEIIADKNRYFVYYTARRDEGKNKKGTLCVAVAVAEKPQGPYTDKGALVCQEMGSI
                     DAFYVRDENDKPFLIWKEDGNDRRQPTWLYAQQLDESGTRLIGKAKKMFRNDAAWENH
                     VVEGSFIIRRDGWFYHFYSGNACCGRGCNYALGVARSKTLLGKWEKNPANPILAANQT
                     WQCPGHGSLVQTPDGRDFLLYHAYRKRSDAFNIGREALLDEVKFRDGWAVINEGRGAS
                     NGAPVPLKDTRQRMVFGLNDEFNENLLAPKWTYPLYNFQNIRLDGGFLTIAPAEKQLT
                     TEKMPEVVVAERTVSGTYTATTRIDYENLSNDEFAGISVYSRRTEAVGISIGNGKIFT
                     WRRAEGKHQEVSGVSLPKASTAIQLRIEAHEGENFQFSFSTDGKAWAAVGDKIVTSNV
                     EGARVALVYNGRTTRSGARFDWLRVEPK"
     gene            complement(4260..5231)
                     /locus_tag="VGB68_19545"
     CDS             complement(4260..5231)
                     /locus_tag="VGB68_19545"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0051501.1"
                     /translation="MAEATPINPATTKSNFRLADYAPTYGALAALILLLILNAVFTPN
                     FAAFDNFRNILVQATPTMLVAVGMTFVISTGGIDLSVGSIMAIASAVAAISLDYGAGA
                     AILIGLGVATAIGAFNGVLIAGFKIQPIIITLALLISGRGIAQVISNGGQLIPFSNPT
                     FEYLGKGVAASVPFQIILLVVVVLLAVFLLRSTIFGRYIIAVGGNEEAARLSGIRVNR
                     TKIAVYALSGLLAGLAGLIYTARLGASDASKVGEGIELDAIAATVVGGTPLTGGRATI
                     IGTIIGALIMQIITTSFNMNGIKFSYSLVIKAAIILLAVYIQRPKLN"
     gene            complement(5341..>6320)
                     /locus_tag="VGB68_19550"
     CDS             complement(5341..>6320)
                     /locus_tag="VGB68_19550"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HEY0051502.1"
                     /translation="WQADTFDPKRIDLELGWAESIGMNTMRVFLHDLAYSQDPEGFKK
                     RLNQFLEISARHKIKPMLVLFDSVWDPNPQIGKQRAPRAGVHNSGWLQSPGAKALANP
                     SEYARLEKYVTDVVGAFAKDRRINSWDIWNEPDNKNGGEYAKQDPPNKVELINKLLPL
                     AFRWARAARPEQPLTSGVWKGDYFKAGNLDETEKIQIENSDFITFHTYEPAEDFEKRI
                     VFLQKYNRPIVCTEYMARPRGSTFVAILPVGKKHDVGMINWGFVQGKSQTNYPWDSWE
                     KPYVEYEPWIWFHDVFRTDGTPYLREETDLIRRLTGKTKTRSAGQSRLR"
BASE COUNT         1453 a         1821 c         1605 g         1441 t
ORIGIN      
        1 ccaaaccgat taaaatcctc gaatcgtttg ccgccagata actgcccgaa ttgctcgcgt
       61 cgaaaatttg ttttctgccg tttgcgtccg tcacggtaag gcgcgcgcct tcgccgcccg
      121 gcgcggattt ttcgccgcgc aaatcgattc cgagccagtg atttttcgtt ccgttgtttt
      181 tcaaaatcac cggcgcggcg tttgtctgag cgataataac gtccgtgtcg ccgtcgttgt
      241 ccaaatcgcc ggaagccatt ccgcgcgccg ctaaatcgat tttgaaaatc tcgcccgccg
      301 ccagtgaaac gttctgaaat tctttgcccg cgttgcgcat caaaagcgaa ggctgtttgt
      361 atttgagaaa atccgtcgtc ttttcaatca cgtcggagac gtggctttgc acgacaaaca
      421 aatcgcgcct gccgtcgctg tcggcgtcga taaattttat tccccaaccc gttcccaatt
      481 gcgtaatctg cgcgacgccg ctcgaccgcg tgacgtaatc aaacgaccaa tcgccgagat
      541 tgcgataaag cgggtaagtt tcgccggaaa aagcagttat cgccacgtcc tgccgcccgt
      601 ctccgtcata atcggcggca tcgacgccca ttccggcaaa acgtttcccg ttttcgtcga
      661 acgcgactcc ggcaattaaa gccgtatctt caaagctgcc gtcgcctttg ttgcgaaata
      721 gatgctgctc ggtattgtcg ttggcgacga aaacgtcagt ccagccgtcg tcgtcgaaat
      781 cggcgaaagc gacgcccaga gatttgcctc gggattgcgc gatgcccgct ttttcgctga
      841 cgttttcaaa agtgccgtcc gccttttggc gaaacaaaag cgaggtcgtc ggcttaaaat
      901 tatccggatg acaataggag cgataaccgg cgcgcgcgtc gccgcaaaaa agcgcgccgt
      961 tttcaaaatc ccagacgaca tagcgcgtaa cgaataaatc gagccgaccg tctttatcaa
     1021 aatcaaaaaa accggcgctt gccgcccagc cttcgacctc gacgccggat tttttcgtcg
     1081 cgtccgtaaa agtgccgttg ccgttgttgc gatacaaaca agcgccgccg taatgcgtga
     1141 cgaacaaatc ggaaaatccg tctttgtcgt aatcgccgac ggcgacgccg aaaccgtagc
     1201 cgacgccttt taagcccgcc ttttcggtca cgtcttcaaa cgcgccgtcc gctcgctgtc
     1261 gataaagccg gttccagaat ttcgcgtttg ttttttccgg cgttttccct ttcggcatcg
     1321 ggtcggaaag ctgcgcgccg ttcgtaaaaa aaacgtcgag ccgaccgtcg ttgtcgaaat
     1381 caatcagacc gacgcctgcg cccatcgttt cgagcagata cttgacggaa gtcggcgagg
     1441 cgttgtgttt gaaatcgatg ccggttcgcg ccgtaatatc ggtaaaaact acgggcgaag
     1501 gaaactgcgg cgcgctggtc gccgggcgtt tgatttcgga gctgtaagat tttcccgttg
     1561 tttgcgcgtt tgcggcgaaa ggcaaaacgc agcaaataaa agcgaataat tttaataaac
     1621 ggtaaaacat tatccgagtc gcgtcaggcg gttttgcaaa atcaccacgg caagcagaat
     1681 cgcgccgcga aaaacaagct gccaccacgg gctgatttgt ccttcgagat tgaaaagatt
     1741 aaaaatcacg ccgagcagca aaacgccgta aagcgtggag acgacgctgc ccttgccgcc
     1801 ggtcaaaagc gtcccgccga cgacgaccgc cgcgattgca tccaattcgc gcccgacgcc
     1861 ttcggtcggc gaacccgtat aaagtctgga agccaaaatc acgcccgcca gtccggctaa
     1921 taatccgcta atcgtataaa ccagaatcgt cacgcggtcg atattgagtc ccatcaggcg
     1981 cgccgcttcg tcgttgtcgc caagcgcgaa aacgtgtcgc ccgaaacgcg tgtaatttaa
     2041 aatcagccag ccgacagcaa atacaattat caaaatcaca atgggaacgg gcagaaaacc
     2101 gatcatgcct tgcccgagcc aggaaaattc cgtcgccgtc gccggaatcc gcacgttttg
     2161 ctcgctggtc acggcaagcg cgatgccgcg cgcggcgatc atcattccga gcgtgacgat
     2221 aaacggctga atccgcgctt tggcgatgac cgttccgtta attaaaccga gaacggtcgt
     2281 cgccgcgacc gccgcgacga gcgccacgac aagccctcgg tcagccagat tagcggcgat
     2341 gacgcccgcg accgccagaa gcgagccgac cgacaaatcg atgccgcccg tcaaaatcac
     2401 gaacgtcatt ccgagcgcga taatgccgag catcgaattc tgccgcaaaa cgttcaaaag
     2461 attttccggc gtcagaaacg cttcataacg cgccgtcgca atcacgcaga ccagaacgag
     2521 cagcaccagc gcgccctgct gctggacgaa agaaccgaag cgctcgcgca tcgtgcgctc
     2581 ttgctgtgcc gaagctgttg cgtcgtgaac cgattgcatt tgttccataa gtttttattt
     2641 tggctcgacc cgcagccaat cgaaacgcgc gcccgaacgg gtcgtcctgc cgttgtaaac
     2701 cagcgcgacg cgcgcgcctt cgacattgct cgtgacgatt ttatcgccga ccgcagccca
     2761 cgcttttccg tccgtgctga acgaaaattg aaaattttcg ccttcgtgcg cctcgatacg
     2821 cagctgaatc gccgtcgaag ctttcggcaa tgaaacgccg gaaacttcct gatgtttccc
     2881 ttcggcgcgc cgccacgtaa aaatcttgcc gtttccgatg ctgatgccga ccgcttccgt
     2941 gcgccggctg tagacggaaa ttccggcaaa ttcatcattg ctcaagtttt cataatcgat
     3001 gcgcgtcgtc gccgtgtaag ttcccgaaac ggtgcgttcg gcgacgacga cttccggcat
     3061 tttttcagtc gtcaattgtt tttcagccgg agcaatcgtc agaaaaccgc cgtcgagccg
     3121 tatgttttga aaattataaa gcgggtaagt ccatttcggc gcgagaagat tttcgttaaa
     3181 ttcgtcgttt aagccgaaaa ccattcgctg ccgcgtatct ttcagcggca cgggcgcgcc
     3241 gttcgaagcg ccgcgtcctt cattaataac cgcccagccg tcgcgaaatt ttacttcgtc
     3301 gagcagcgct tcgcgcccga tgttgaaagc gtccgagcgt tttcgataag cgtgataaag
     3361 cagaaaatcg cgtccgtccg gagtttgcac taaactgccg tgaccgggac attgccacgt
     3421 ctgatttgcc gccagaatcg gattcgcggg atttttttcc cattttccca gaagcgtttt
     3481 cgagcgcgcc acgccgagcg cgtaattgca gccgcgaccg cagcaggcat tgcccgaata
     3541 aaaatgataa aaccagccgt cgcgccgaat gataaacgag ccttcgacaa cgtgattttc
     3601 ccaagccgcg tcgttgcgaa acatcttttt cgctttgccg ataagccgcg taccgctttc
     3661 gtccaattgc tgcgcgtaaa gccacgtcgg ttgccggcgg tcgttgccgt cttctttcca
     3721 aatcaaaaac ggcttgtcgt tttcgtcgcg gacataaaac gcgtcaatcg agcccatttc
     3781 ctgacagacg agcgcgcctt tgtccgtata aggtccctgc ggcttttcgg cgacggcgac
     3841 ggcgacgcaa agcgtgcctt ttttattttt tccctcgtcg cgccgcgccg tgtaataaac
     3901 aaaataacga tttttgtcgg cgataatttc cggcgcccag aaatcgcctt tcgcccacgc
     3961 cggtttttcg ccgaaaaccg cgccgatgat ttcccagtta atcaaatctt tcgagtgcag
     4021 aagcggaaaa tgcggcgacc agccgccggt cgtcgccgtc gcgtaataat cttcgcccac
     4081 gcggattacc gacgggtcgg gaaaatcgcc cgcaattacg ggattaacgt aagtctgcgc
     4141 ttgagatgga aggacgaaaa gaaaagctaa ccacagataa acgcagatag atgcagatat
     4201 tttttgattt aatctgcttt catccgcgtt tatctgtggt ttcattttgt atttcatttc
     4261 tagttcagtt tcgggcgctg gatgtaaacc gccagcaaaa taatcgccgc tttaatgacg
     4321 agcgaatagg aaaattttat gccgttcata ttaaagctgg tggtgataat ctgcattatc
     4381 agcgcgccga taatcgtgcc gataatcgtc gcccgaccgc cggttaaagg cgttccgccg
     4441 acgaccgtcg cggcaatcgc atccagctcg attccctcgc cgactttcga ggcgtcgctc
     4501 gcgccgagcc gcgccgtgta aatcaatccg gcgagtccgg cgagcaagcc gctcaaggcg
     4561 taaaccgcga ttttcgtacg gttgacgcgg atgccggaga gccgcgccgc ttcttcgttt
     4621 ccgccgacgg cgatgatgta acgcccgaaa atcgtcgagc gcagcaaaaa aaccgccagc
     4681 agaacgacga cgacgagcag gataatctga aacggaacgc tcgccgccac gcctttgccc
     4741 aaatattcaa aagtcggatt gctgaacggg ataagctgac cgccgttgct gataacctgc
     4801 gcgatgccgc gcccggaaat caaaagcgcc agcgtgatga taatcggctg aatcttaaaa
     4861 ccggcaatca gcacgccgtt aaatgcgccg attgccgtcg ctacgcctaa accaatcaaa
     4921 atcgccgcgc ccgcgccgta atccagcgaa atcgccgcca ccgccgaagc aatcgccatt
     4981 atcgagccga ccgacaaatc gatgccgccc gtcgaaatca cgaacgtcat cccgacggcg
     5041 accagcatcg tcggcgtcgc ctgcaccaga atgtttcgaa aattgtcgaa agcggcaaaa
     5101 ttcggcgtaa aaacggcgtt cagaatcagc aataaaatta atgcggcgag cgctccgtaa
     5161 gtcggcgcgt aatccgccag cctgaaattg ctttttgtcg tcgccgggtt gattggtgtc
     5221 gcttctgcca taatttttaa tatttgcctg ttcgaagaca gtttaagtga aaaaacttta
     5281 gcccgtacaa ctacgctaaa ctgaaatctg aaactcgaag ctctcgcgtt caggcgaccg
     5341 ctaccgtagg cggctctgac ccgcgcttct ggttttggtt ttgccggtca gccgtctaat
     5401 caaatcggtt tcttcgcgca gatacggcgt cccgtccgtg cggaaaacgt cgtgaaacca
     5461 aatccacggc tcgtattcga catacggttt ttcccacgaa tcccaagggt aattcgtctg
     5521 cgatttgccc tgcacaaagc cccagttaat catcccgaca tcgtgttttt tgccgacggg
     5581 cagaatggcg acaaacgtcg agccgcgcgg gcgcgccata tattcggtgc agacaatcgg
     5641 tcgattgtat ttctgcaaaa acacgatgcg cttctcgaaa tcttccgccg gttcatacgt
     5701 gtgaaaagtg atgaaatccg agttttcgat ttgaattttt tccgtttcgt ccagatttcc
     5761 cgctttgaaa taatcgcctt tccagacgcc ggacgttaaa ggctgttcgg gtcgcgcggc
     5821 gcgcgcccat cgaaaggcga gcggcaaaag cttgttgatt aattcgactt tgttcggcgg
     5881 gtcttgtttg gcgtattcgc cgccgttttt attgtccggc tcgttccaga tatcccacga
     5941 atttatgcgt ctgtctttcg caaacgcgcc gacgacgtcc gtgacgtatt tttccagacg
     6001 cgcgtattcc gacggattcg ccagcgcttt tgcgccgggc gattgcagcc agccggaatt
     6061 gtgaacgccg gcgcgcggcg cgcgttgttt gccgatttgc ggattcgggt cccagaccga
     6121 atcgaacaaa acgagcatcg gcttgatttt atggcgcgcg gaaatttcca gaaattgatt
     6181 gagccgtttt ttaaagcctt ccgggtcttg gctataagcc aaatcgtgca gaaaaacgcg
     6241 catcgtgttc atcccgatgc tttccgccca gcccagctcc aaatcaatcc gtttcgggtc
     6301 gaaagtgtct gcctgccaca
//
LOCUS       DASWNE010001020         2445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_105264, whole genome shotgun sequence.
ACCESSION   DASWNE010001020 DASWNE010000000
VERSION     DASWNE010001020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105264"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VGB68_19555"
     CDS             complement(<1..561)
                     /locus_tag="VGB68_19555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051503.1"
                     /translation="MKNVFYSLLFVLSAFGICPAQSVGSVSSEVVKIEAQPAKGFAYP
                     YYLYVPKAVREKAGTAKKPRTILVIPNNSGKTSDDFSVHEADVRRKIRNNSEIADRLE
                     VALLMPVFPRPQTDWKIYTHALDRDAMTTGKKEFARFDLQLVAMLDDARAKLAKEKLK
                     FDKRVFMLGWSASAMFANRFTFLHPKR"
     gene            complement(744..>2445)
                     /locus_tag="VGB68_19560"
     CDS             complement(744..>2445)
                     /locus_tag="VGB68_19560"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY0051504.1"
                     /translation="VTYAELRREVEACAAGLRLDGLKKGDSVGIHLPMMIETVVALLA
                     INRIGAIAVPVFSGYGVSAIESRLNAVGAKALFTCDGFPRRGKNFDAFEIAEKAVENC
                     PSVEKVYIVNRILKGLSYSFINAEHKYSYLYFDELLDMGESGLIKVEDYAGKSESAKR
                     KIENEAKKKAENLRNPELTLAEDPLIILYTSGTTGKPKGIAHTHCSFPIKAAQDMAFG
                     TDVGRGTRISWITDIGWMMGPWLIYGALIRGATMVIYDGAPDYPAPDRMWEFCAKHKV
                     EILGISPTLVRALAAHGDDLPKKHDLSALRAFASTGEPWNPAPWWWLFEKVGDSRIPI
                     INYSGGTEISGGILMGNPLLPIKPCSFPAPCPGIAADILNEKGEPVAANQVGELAIKQ
                     PWIGMARGFWQERERYLETYWNRFPGIWIHGDWAMKDADGHWFILGRSDDTLKVAGKR
                     VGPAEVESLLVGDSRVAEAAVIGVPDEMKGTAMVAFCVLKTEATANLESELKTVVAKD
                     MGKPLAPSRIHFVAALPKTRNAKVMRRVIRAAYLGEDAGDLSALENPNAVEEIKRLLN
                     "
BASE COUNT          566 a          683 c          593 g          603 t
ORIGIN      
        1 acgcttcgga tgcagaaacg taaaacgatt ggcaaacatc gccgaagccg accatccgag
       61 cataaaaacc cgcttatcaa atttcagttt ttcctttgcc agcttcgccc gcgcatcgtc
      121 gagcattgcg acgagctgca aatcgaaacg cgcaaattct tttttgccgg tcgtcatcgc
      181 atcgcggtcc agagcgtgcg tgtaaatctt ccaatcggtc tgggggcgcg gaaaaacagg
      241 catcagaagg gcgacttcga gccggtcggc gatttcgctg ttattcctga ttttccgcct
      301 gacatccgct tcgtgaacgc tgaaatcgtc gctggttttg cctgaattgt tcggaatgac
      361 gagaatcgtg cgcggcttct tcgctgttcc ggctttttcg cgcaccgcct tcggcacgta
      421 tagataatac ggataagcaa aacctttggc gggctgagct tcgattttga cgacttcgga
      481 tgagacactg ccgacgctct gcgccggaca gatgccgaaa gccgacaaaa caaataataa
      541 agaataaaac acatttttca ttacaaattt cacactccga ttctaaaagt tcgtgttttt
      601 ttcaataatc tcaaaagtct tacgcgctct ttctgaaagg cggataattt ctttccggta
      661 ataagtgcgg cgaatcagac gcgttaaagc ctaagctttc cttagcgaaa atttaaaatt
      721 aaccgtctgt cttgaaaagg aaattaattc aaaagtctct taatttcctc gaccgcgttt
      781 ggattttcca gcgccgataa atcgcccgcg tcttcgccta aatatgcggc gcgaatgacg
      841 cggcgcatta ctttggcgtt gcgcgttttc ggcagcgcgg cgacgaaatg aatccgcgaa
      901 ggcgcgagcg gtttgcccat atcttttgcc acgaccgttt tcagctcgga ttcgagattc
      961 gccgtcgctt ccgtttttaa aacgcagaac gcgaccatcg ccgtgccttt catttcgtcc
     1021 ggaacgccga tgacggcggc ttcggcgact ctgctatcgc cgaccagaag gctttcgacc
     1081 tcggctggtc cgacgcgttt tccggcgacc ttcagcgtgt cgtccgagcg tccgagaata
     1141 aaccagtgcc cgtccgcatc tttcatcgcc caatcgccgt gaatccagat gccgggaaaa
     1201 cggttccagt aagtttccag atagcgctcg cgttcctgcc agaaaccgcg cgccattcca
     1261 atccagggct gctttatcgc cagctcgccg acctgattgg cggcgaccgg ttcgcctttt
     1321 tcatttaaaa tatccgccgc gatgccgggg cacggcgcgg gaaacgagca cggctttatc
     1381 ggcaaaagcg gatttcccat caaaattccg cccgagattt ccgtgccgcc cgaatagttg
     1441 ataatcggga tgcgcgagtc gccgaccttc tcgaaaagcc accaccaggg cgcgggattc
     1501 cacggctcgc ccgtcgaagc gaaggcgcgc aaagcggaaa ggtcgtgttt tttcggcaaa
     1561 tcgtcgccgt gcgcggcgag cgcgcgcacc agggtcggcg agattcccaa aatctcgact
     1621 ttgtgtttcg cgcaaaattc ccacatccgg tcgggcgcgg gataatcggg cgcgccgtcg
     1681 tagatgacca ttgtcgcccc gcgaatcaaa gcgccgtaaa tcagccacgg tcccatcatc
     1741 cagccgatgt cggttatcca cgagatgcgc gtgccgcgcc cgacgtccgt gccgaaagcc
     1801 atatcctgcg ccgcttttat cggaaagctg caatgcgtgt gcgcgatgcc cttgggtttt
     1861 ccggtcgtgc cggaagtgta aagaataatg agcgggtctt cggcaagcgt cagttccggg
     1921 ttacgcaaat tttcagcttt tttcttcgct tcattttcta tttttctttt agcagattca
     1981 ctcttgccgg cgtaatcttc gactttgata agtccgcttt cacccatatc taaaagctcg
     2041 tcgaaatata gatatgaata tttatgctcg gcgttaatga atgagtaact taaacctttc
     2101 aaaattcggt tgacaatgta aaccttctcg accgacggac agttttctac tgccttttca
     2161 gcgatttcaa aagcatcaaa gtttttcccg cggcgcggaa aaccgtcgca ggtaaaaagg
     2221 gcttttgcgc cgaccgcatt taatcttgat tcaatcgccg aaactccata accggaaaaa
     2281 acgggaacgg caattgcgcc gattcgattg attgccaaaa gcgcaacgac cgtttcaatc
     2341 atcatcggca aatgaatgcc gaccgaatcg cctttcttta atccatccaa acgcaaacct
     2401 gccgcgcagg cttcgacctc tcgccttaat tccgcataag taacc
//
LOCUS       DASWNE010001021         1591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_528132, whole genome shotgun sequence.
ACCESSION   DASWNE010001021 DASWNE010000000
VERSION     DASWNE010001021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528132"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VGB68_19565"
     CDS             <1..608
                     /locus_tag="VGB68_19565"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY0051505.1"
                     /translation="RFYLIDAIKDYIESGKIRAYSINSVNRYSLLNKESHPAWKVEML
                     SRYDRYIMEEVLPLIRNECGADAKPLTTGASLGALLAANTYFKHSDQFRGTIAMSGSY
                     NIYNYLENYYDDNVYFNNPMMYLKNLNDDYHLPRLQKADSIIISTGQGAYEAPDRSRE
                     FSALLHSKGIPHWLDVWGHDVNHDWVWWRKMLPYYLGKLVG"
     gene            complement(733..>1591)
                     /locus_tag="VGB68_19570"
     CDS             complement(733..>1591)
                     /locus_tag="VGB68_19570"
                     /inference="COORDINATES: protein motif:HMM:NF024813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY0051506.1"
                     /translation="TKQFTDMKWGTANPIMLRDNDFGIVRLRSFGGYSMRVVDPQTFI
                     KEVAGTNAHFQTEDIDAQLKRAIVSEFSDAIGEMKIPALDLAAQYKELGEAIRAKINE
                     DFRTYGIEVTKFYVENISLPPEVEAAIDKRSSMGALGSAEQYMKFQAADALRDAAQNE
                     GGGAGLGAGLGAGFAVGNQMANVFGQQGGQGGGQQQTVQPQAQMIPCPTCGKSNTSGT
                     KFCGECGGKMEVAKVPCVKCGAELREGAKFCSECGSSQEKAKCENCQAQLAPGAKFCP
                     ECGTKTEGS"
BASE COUNT          356 a          436 c          400 g          399 t
ORIGIN      
        1 aaagatttta tctgattgac gccatcaagg attacatcga aagcggcaaa attcgcgctt
       61 attcgattaa ttcggtcaac cgctacagct tattgaacaa ggaatcgcat ccggcttgga
      121 aagtcgagat gctctcgcgc tatgacagat atattatgga agaagttttg ccgctgattc
      181 gcaacgaatg cggagcggac gcgaaacctc tgacgaccgg cgcgagcctc ggcgcgctgc
      241 tggcggcaaa cacttatttc aaacattccg accaatttcg cggcacgatt gcgatgagcg
      301 gcagctacaa catttacaat tatctggaaa attattacga cgacaacgtt tatttcaaca
      361 acccgatgat gtatctgaaa aatctgaacg acgattatca tctgccgcgc ctgcaaaaag
      421 ccgattcaat catcatctcg accgggcagg gcgcgtatga agcgccggac agaagccgcg
      481 aattttccgc gcttcttcat tccaaaggca ttccgcactg gctcgacgtc tggggacacg
      541 acgtcaatca cgattgggtc tggtggcgca agatgctgcc gtattatctg ggcaagctgg
      601 tcggctaatt aactgataat tgataactga taactgatag tgaaaaaatg aaaagcgaga
      661 actgaataaa tagttctcgc ttttcttttt ttactttgcg gttttcagtt atcaactatc
      721 agttgtcggt tatcaactgc cttccgtttt cgttccgcat tcgggacaga attttgcgcc
      781 cggcgcgagc tgcgcctgac aattctcgca tttcgctttt tcctggctcg aaccgcattc
      841 cgaacagaat ttcgcgcctt cgcggagttc cgcgccgcat ttgacgcagg gcactttggc
      901 gacttccatt ttgccgccgc attcgccgca gaattttgtt cccgacgtat ttgatttgcc
      961 gcacgtcgga cacggaatca tctgcgcctg cggctgaacg gtctgctgct gaccgccgcc
     1021 ctgaccgccc tgctgaccga aaacgttcgc catctgattg ccgacggcaa agcccgcgcc
     1081 caagcccgcg ccgagtcccg cgccgccgcc ttcgttctgc gccgcgtcgc gcaaagcgtc
     1141 agccgcctga aatttcatat attgttcggc gctgccgagc gcgcccatcg agctgcgttt
     1201 atcgattgcc gcttcgactt cgggcggaag cgaaatattt tcgacgtaga acttcgtgac
     1261 ctcgattccg taagtgcgga aatcctcgtt gattttggcg cgaatcgcct cgccgagttc
     1321 cttgtattgc gccgctaaat ccagcgccgg aattttcatc tcgccgatgg catcggaaaa
     1381 ttccgaaacg atggcgcgtt ttaattgcgc gtcgatatct tcggtttgaa aatgcgcgtt
     1441 tgtgccggcg acttccttga taaacgtctg cgggtcgacg acgcgcatcg aatagccgcc
     1501 gaaagagcgc aggcggacga tgccgaaatc attgtcgcgc agcataatcg ggtttgccgt
     1561 gccccatttc atatcggtaa actgcttggt g
//
LOCUS       DASWNE010001022         6188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_599421, whole genome shotgun sequence.
ACCESSION   DASWNE010001022 DASWNE010000000
VERSION     DASWNE010001022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6188
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599421"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /gene="gmd"
                     /locus_tag="VGB68_19575"
     CDS             <1..971
                     /gene="gmd"
                     /locus_tag="VGB68_19575"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR01472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEY0051507.1"
                     /translation="EVHGIIRRASTFNTERIDHLYQDPHEREKTFILHYGDLSDGTGL
                     RRILEKIQPDEIYNLGAQSHVKVSFEQPEYTADIVGTGTLRLLEAVRDSLSAGGKEIR
                     FYQAGSSEMFGGAPAPQNEKTPFYPRSPYAVGKVAAHWFAVNYRESYGMFICNGILFN
                     HESERRGETFVTRKITRALARIKAGLQEKLYLGNLDAVRDWGHTADYVEAMWLMLQQP
                     EPEDYIVATGEAHSVRDFLDEAFGLAKLDWRNYVEIDPGYFRPAEANYLLGDAAKIRE
                     KLGWQPKIKFKELVRLMYEHDLELARQEKVLKDNGHVVALRGRVNG"
     gene            964..1989
                     /locus_tag="VGB68_19580"
     CDS             964..1989
                     /locus_tag="VGB68_19580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HEY0051508.1"
                     /translation="MGKDDKIFVAGHRGLAGSAILRKLESEGYRRILTRTRAELDLTI
                     QAQVENFFAAEKPEIVFLAAGKVGGIWANNSQPADFIRDNLLIQTNAVDAAHRHGVKK
                     FIFLGSSCIYPKHAPQPLKEEYLLTSALEPTNEAYAIAKIAGIKMCQAYRRQYGFDSI
                     CLMPTNLYGRGDNFDLANSHVLPALLRKFHEAKMNGYESVKIWGTGTPQREFLHADDL
                     ADACHFLMRQPSEIVNAAAPDGIINVGVGEDVSILELAEIISRTVQFGGNLEFDSAKP
                     DGTPRKLLDVSRLDALGWKAKISLGEGIAQTYHWYKKAYNRDSPELEPESNQINSNRE
                     QNRIGVS"
     gene            2261..3421
                     /locus_tag="VGB68_19585"
     CDS             2261..3421
                     /locus_tag="VGB68_19585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0051509.1"
                     /translation="MSRSLFRFAFASSCTDFWGGSEELWAGAAHIFAGAGHRVRAFKT
                     NVDRREKQIIKLEAANCPVKDLQGLPPKPLRLLNRFLPHDRQPLHKNYAQKSLDKALR
                     SFQPHLTVVSQGINFDGVYLAEVCRRQKLPYVVVAQKAVDFLFPADEERAAVRAVYES
                     AVKCFFVSRHNLNLTEEQIGAVLPNAEIVFNPFAVPFENSLSWNEEDGNVRLACVARL
                     FIIDKGQDILLRVLAMEKWKKRNVEVVFFGEGVNKQALVEMARLLEVRNVKFAGYVGD
                     VEHIWKESHALVLPSRSEGLPLALVEAMLYGRPSIVTDVGGMAEIIDDNVTGFVAAAP
                     TAQAFDEALERAWMQRRNWRQIGIRAADSIKKIVPREPCRQFADRLLELTKE"
     gene            3440..4441
                     /locus_tag="VGB68_19590"
     CDS             3440..4441
                     /locus_tag="VGB68_19590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY0051510.1"
                     /translation="MNHKLVSVIIPTFNRRSLVVEAIRSAQAQSYPSKQIIVIDDGSI
                     DDTAQAVAQFEGVEYYRQENKGQAAARNLGLQYAKGEYIASLDSDDVWNAEFLTDGIN
                     CLEKHELDFVFLNWTSSDGKENFLDFWERKKKWRNFTESQDSQWFLLNSAQVRRLFLE
                     TCPSPSSSLLIRRSSLTSKWNEEMIIADDWFLILEMVLSKPCRAAFTLSPYWLKRVFN
                     DNIYDGKELLEISKNLGLHDERLMARHFSSQLTTEEKNILRERLIHHHLNFGRLSWKF
                     EGVSKNVLIGVGAAFALAPIGFGIYITKIFIDHIKNRRNVETLPNSATAKIFRPEEK"
     gene            complement(4560..>6188)
                     /locus_tag="VGB68_19595"
     CDS             complement(4560..>6188)
                     /locus_tag="VGB68_19595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051511.1"
                     /translation="LRCSTTGAAPCTFSGLIINRFQEGGIRIDATARGAIIGSFIGTN
                     SAGAAALGNINYGILILDTSALGSIVGGISPGERNVISGNSGTGVTLSAGADSVLIIG
                     NYIGTDKNGAADLGNTSHGVLIANSSSNSVDGNIISGNNGSGVTIQQDFGFTASGNST
                     VKNFIGVNADGNSALGNNGSGVLLQAAGNFIGSDSSADRNVIAGNGANGISISTNFAT
                     GNSILGNRIGVGANGTTSIPNFDNGVQISNSAAGNTIGDPNGATLGATPACTGACNII
                     ANNGNALSTGARAGIYLDSTAGAGNAIRANSIFNNTGLGIDLQATGATANDLNDPDTG
                     ANNQQNKPVINAANTSGFISGTLNSTAGTTFAIDFFRNSLPDTALTGEGRTYIGTVNC
                     TSVSTASCAISGNNYTFVFTTGVPLSVGQFVTATATATGTAFAPQAAGDTSEHSDAAS
                     VVLAPPTAAGVTVAGRILSGKNRGVSNAFVSLTDSSGEIRVARTNPFGYYQFTEVRAG
                     ETYIVGVRHRSYQFTPQIVTVNEELADVDFMPNR"
BASE COUNT         1479 a         1559 c         1736 g         1414 t
ORIGIN      
        1 atgaagttca cggcatcatc cgccgcgcgt ccacctttaa caccgagcgg atcgaccacc
       61 tgtatcaaga cccgcacgag cgggaaaaaa cgtttattct tcactacggc gacctgtcgg
      121 acggaacggg tctgcggcga atcttggaaa aaattcagcc ggacgagatt tataatttag
      181 gcgcgcagtc gcacgtcaaa gtttccttcg agcagcccga atacaccgcc gatattgtcg
      241 ggacgggaac tcttcggctg ctcgaagccg tgcgcgattc gctctcggcg ggcggaaagg
      301 aaattcgttt ttatcaggcg ggctcgtccg aaatgttcgg cggcgcgccc gctccgcaaa
      361 atgaaaaaac tcctttttat ccgcgcagtc cgtatgcggt cgggaaagtc gccgcgcact
      421 ggtttgccgt caattaccgc gagtcttacg ggatgtttat ctgcaacggc attcttttta
      481 accacgaatc cgagcggcgc ggcgaaacgt ttgtcactcg taaaattacg cgcgctctgg
      541 cgcggattaa agccggtttg caggaaaaac tttatttggg caatctcgat gccgtgcgcg
      601 attggggaca cacggcggat tatgtcgaag cgatgtggct gatgctgcaa cagcccgaac
      661 cggaagatta catcgtcgcg accggcgaag cgcattcggt gcgcgatttt ctggacgagg
      721 cttttggttt ggcgaagctc gactggcgga attacgtcga gatagacccc ggatattttc
      781 gtccggcgga agcgaattat ctgctgggcg acgcggcgaa aatcagagaa aagctcggat
      841 ggcagccgaa aataaagttt aaggaactgg tcaggctgat gtatgaacac gaccttgagc
      901 tggcgcggca ggaaaaggtt ttaaaagata acggtcacgt cgtcgcgctg agaggtagag
      961 taaatgggta aggacgataa aatattcgtt gccgggcacc ggggcttggc tggctcggcg
     1021 attttgcgca agctcgaatc ggaaggttac cggcggattc taacccgcac tcgcgccgag
     1081 ctggatttaa cgattcaagc gcaggtcgaa aatttctttg cggcggagaa acccgaaatc
     1141 gtctttctgg cagccggaaa agtcggcggc atctgggcta acaactcgca accggcggat
     1201 tttatacgcg ataatctttt gatacaaacg aacgctgtcg acgcggcgca tcgtcacggc
     1261 gtaaagaaat ttattttcct cggctcgtcc tgtatttatc cgaagcacgc gccgcagcct
     1321 ctgaaggaag aatatctttt aacctcagct ttagagccga ccaacgaagc ttacgcgatt
     1381 gctaaaatag ccggaataaa aatgtgtcag gcttatcgcc ggcaatacgg attcgacagc
     1441 atctgcctga tgccgaccaa tctgtacggg cgcggcgaca attttgattt ggcaaattcg
     1501 cacgttctgc cggcgcttct gcgaaagttt cacgaggcga aaatgaatgg ttatgagagc
     1561 gtcaaaatat ggggaacggg aacgccgcaa agagagtttc tgcacgcgga cgatttagcg
     1621 gacgcgtgtc attttttgat gcggcagccg tcggaaatcg ttaatgccgc cgcgcccgac
     1681 ggaattatta acgtcggcgt cggcgaagac gtaagcattc tcgaattagc cgaaattatc
     1741 agccggacgg tgcagtttgg cggcaatctg gagtttgatt cggcgaaacc ggacggaacg
     1801 ccgcgcaagc tgcttgacgt aagccggctc gacgctctgg gctggaaagc aaaaatttct
     1861 ctcggcgaag gcatcgcgca aacttatcat tggtataaaa aggcgtataa tcgtgacagc
     1921 ccggaattag agccggaatc caatcaaata aattccaacc gggaacaaaa tcgaatcggg
     1981 gtttcttaaa tcgaatttta ttgaaagccg agccgggcaa aatacgaagc gggaaaatta
     2041 agtcagaaaa tcgcccggac agttgaatcc gtttttagtt tttccgcgaa ttcactaaca
     2101 cggtcaattg cgaccaaatt tacgaataag taaacttttg tgcaaaattt ccgataacaa
     2161 tcactgaggg cgggcgggaa gtattcagtt tatttaacat ttaataataa gcagctatga
     2221 gggttgacga agatatttga aacagcggta agggggatta ttgagccgga gtctttttcg
     2281 atttgcgttt gccagctcct gcaccgattt ctggggcggg agcgaagagc tctgggcggg
     2341 agccgcacat atctttgccg gtgccgggca tcgggtgcgg gctttcaaga ccaatgtcga
     2401 ccggcgcgaa aagcagataa ttaaacttga ggcggcaaat tgcccggtca aggatttaca
     2461 aggtctgccg ccgaaaccgc tccgcttatt aaacagattt ctgccccacg accggcagcc
     2521 tctccataaa aactacgctc aaaaatcgct ggataaagct ttgcgaagtt ttcagcccca
     2581 tttgaccgtc gtttcgcagg gaataaattt cgacggcgtt tatttggcgg aagtttgccg
     2641 ccggcagaaa cttccatacg tcgtcgtggc gcaaaaagcg gtggattttt tgtttccggc
     2701 ggacgaagag cgcgccgccg tccgggcggt ctacgaatcg gcggtaaaat gttttttcgt
     2761 atcgcggcac aatctgaatt tgaccgagga gcaaatcgga gctgtgctgc ccaacgccga
     2821 aatcgttttt aacccgtttg cggtgccttt tgaaaattcg ctgtcgtgga acgaagagga
     2881 cggcaacgtc aggctggctt gcgtggcgcg gctgttcata atcgataagg ggcaggacat
     2941 tctgctgcgc gtgctggcga tggagaaatg gaaaaagcgc aacgtggaag tcgtgttttt
     3001 cggcgaagga gtcaacaaac aagccctcgt cgagatggct cgtctgctgg aggtgcgcaa
     3061 cgtaaaattc gccggttatg tcggcgacgt cgaacatatc tggaaagaaa gccacgcgct
     3121 ggtgctgcct tcgcgcagcg aaggattgcc gctggcgctg gtcgaagcga tgttgtacgg
     3181 tcgcccgtcg attgtgaccg acgtcggggg aatggctgaa atcattgacg ataatgtgac
     3241 aggtttcgtc gccgccgcgc cgaccgcgca agcctttgac gaagcgctgg agcgggcgtg
     3301 gatgcagcgg cgcaattggc ggcagatagg aattcgcgcc gccgattcga ttaaaaaaat
     3361 tgttccgcga gagccttgcc ggcagtttgc cgatagactt ttggaactga caaaagaata
     3421 aactgactga ctctttacaa tgaatcataa actggtctcc gtcattatcc cgacatttaa
     3481 tcgccgctcg ctcgtggtcg aagccatccg cagcgcgcaa gcccagagct acccgtcgaa
     3541 acagataatc gtcatcgacg acggctcgat tgatgacacg gcgcaggcgg tcgcgcagtt
     3601 tgaaggcgtc gagtattacc gtcaggagaa caagggacag gcggcggcgc gcaatctggg
     3661 cttgcaatac gccaaaggcg aatacatcgc ttctctggat tcggacgacg tgtggaacgc
     3721 cgagtttctg accgacggca ttaattgtct ggagaaacac gagctggatt tcgtttttct
     3781 caactggacg agctcggacg gaaaggaaaa ttttctggat ttctgggagc gcaagaaaaa
     3841 gtggcgaaat tttaccgaga gccaggacag tcagtggttt ttgctgaatt cggcgcaagt
     3901 ccgccggttg tttctggaaa cctgcccgtc gccttcttcg tcgcttttaa tccgtcgcag
     3961 ctcgctcact tctaaatgga acgaagagat gattattgcc gacgactggt ttctgatttt
     4021 ggaaatggtg ctttccaaac cctgccgcgc cgctttcacc ttatcgccgt actggctgaa
     4081 gcgcgtcttc aacgacaaca tttacgacgg caaggagctt ctggaaatca gcaaaaatct
     4141 cgggctccac gacgaacggc tgatggcgcg gcatttcagc tcgcaattaa cgaccgagga
     4201 aaaaaacatt ctccgtgaaa gactgattca tcatcatctt aatttcgggc gattaagctg
     4261 gaaatttgaa ggcgtctcaa aaaatgtgct tatcggcgtc ggcgcggctt ttgccctcgc
     4321 gccgatcggg ttcggcatct atattacgaa aatatttata gaccacatta aaaatcgccg
     4381 gaatgtggag accctgccga attcggcgac cgccaaaatt ttccgtcccg aagaaaaata
     4441 accggaagcg agcgtttcga atcatcatcg cgaagaaaca aagaaaggca ggaataaacc
     4501 cgcctttctt tgtttcttcg cgatttgttt atcgcaaatt ccaaagccgt tcgggcggct
     4561 tatctgttcg gcataaagtc cacatcggca agctcttcat taaccgtaac gatttgcgga
     4621 gtgaattgat acgagcgatg cctgacgccg acgatgtagg tctcgcccgc gcggacttcc
     4681 gtaaactggt aataaccgaa cggatttgtt ctggcgacgc gaatttcgcc gctgctgtcg
     4741 gtcaagctga cgaaggcgtt tgaaacgccc ctgttttttc cggacaagat tcttccggca
     4801 acggttacgc ctgccgcagt cggcggcgca aggacaacgg aagccgcgtc ggaatgttcc
     4861 gaagtgtcgc cggcggcttg cggcgcaaaa gctgttccgg tcgccgtcgc ggtcgccgtc
     4921 acgaactgtc cgaccgaaag cggcacgccc gtcgtaaaaa caaacgtata attatttccc
     4981 gaaatcgcac acgaggcggt ggaaacggat gtgcagttaa ccgtgccgat ataggtgcgt
     5041 ccttcgccgg tcagagcggt atcgggcagc gaattacgga aaaaatcgat ggcgaaagtc
     5101 gtcccggcgg tgctgttgag cgttccggaa ataaacccgc tcgtgttggc ggcatttatg
     5161 acgggtttgt tttgctgatt gttcgcgccc gtgtccgggt cgttaaggtc gttagcggtc
     5221 gcgccggtcg cctgcaaatc gatgcccaag cccgtgttgt tgaaaatcga attggcgcga
     5281 atcgcattgc ccgcgcccgc cgtcgaatca agataaattc cggctcgcgc gccggtcgaa
     5341 agcgcattgc cgttgttggc gatgatattg cacgcgcccg tgcaggcagg cgttgcgccg
     5401 agggtggctc cgttcgggtc gccgatggtg ttgcccgccg cgctgtttga aatctgaacg
     5461 ccgttatcaa aattcggaat cgaagtcgta ccgttagctc cgacgccgat tctatttccc
     5521 agaatggaat ttccggtcgc aaaattcgtg ctgattgata tgccgttcgc gccgttgccg
     5581 gcaatcacgt tgcgatccgc cgatgaatcg gagccgatga agttgcccgc cgcctgaagc
     5641 agcacgccgc ttccgttatt gccgagagcc gaattgccgt cggcgttaac gccgatgaag
     5701 tttttgaccg tgctgttgcc ggatgccgta aagccgaagt cctgctgaat ggtgacaccg
     5761 cttccgttat tgccggaaat gatattgccg tctaccgagt tgctgcttga attggcaatc
     5821 aaaacgccgt ggctcgtatt acccagatcg gcagccccgt ttttgtcggt gccgatgtaa
     5881 tttccgataa tcaaaaccga atcggctccg gctgaaagcg taacgcccgt gccgctattg
     5941 ccggaaatca catttcgttc acccggagat attccgccga caatgcttcc gagcgcgctt
     6001 gtgtcaagaa tcaaaatacc gtaattgatg tttcccagag ccgccgcgcc ggctgaattc
     6061 gtgccgataa agcttccgat aatcgctccg cgggctgtcg cgtcgatgcg aattccgcct
     6121 tcctgaaaac gattgataat cagcccgctg aaagtacagg gcgcggctcc ggtagtcgag
     6181 catcgcaa
//
LOCUS       DASWNE010001023         2029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_599482, whole genome shotgun sequence.
ACCESSION   DASWNE010001023 DASWNE010000000
VERSION     DASWNE010001023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599482"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VGB68_19600"
     CDS             complement(<1..188)
                     /locus_tag="VGB68_19600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051512.1"
                     /translation="MADEHTLVEKSRIMDGARMSRALSRLASEIVEDNQGAKGLYLVG
                     IRRRGVPLAEKLVEKIENL"
     gene            260..838
                     /locus_tag="VGB68_19605"
     CDS             260..838
                     /locus_tag="VGB68_19605"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEY0051513.1"
                     /translation="MALPKTYPIFTIAEYLDYERYAESRHEFLDGTVYAMAGESPRHS
                     TICFNLYATIGSQLRGKRCRGFSPNMKIASSNKGLFSYPDLAIVCGEPLYHDDKQDVL
                     TNPRVIFEVLSPSTQSYDRGEKLLRYTNTIETLTDYVLIAQDAPLVEHFQKQADGNWL
                     KFEISGLESILHLAAVECEIPLSELYYLVDFS"
     gene            790..1944
                     /locus_tag="VGB68_19610"
     CDS             790..1944
                     /locus_tag="VGB68_19610"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003645.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEY0051514.1"
                     /translation="MRDSAFRALLSGRFFVKLYQSLIRPILFKMPTETAHELGINALK
                     LGLGNEFLQKRFAGKYACDEFGELERFNLKFKNPFGVAAGFDKNAVVASQLCALGFGF
                     VEVGTVTFEPQKGNPKPRLFRLPEDKALINRAGFNNEGTPAVVERLKNRRPDCVLGVN
                     IGKNKDVANEAATENYLSSFDLAHAVADYIAVNVSSPNTPNLRELQKAETLEELLGAL
                     QTRNAELNQLNKKNLPLLVKIAPDLSEAEIESVTDVCLRLNLDGIIATNTTVSRNNLR
                     ACAAEIERIGNGGLSGKPLNARSTEVISKIHRYSKGKLPVVGVGGIFTPQDAFEKIAA
                     GACLIQAYTGFVYQGLAFARDINFGLAKILREKGFKSLDEAIGTRANLRQ"
BASE COUNT          526 a          518 c          518 g          467 t
ORIGIN      
        1 agattttcga ttttctcgac gagcttttcg gcgagcggca cgccgcgccg ccgaatgccg
       61 accagataca agcctttcgc gccctgatta tcctcgacga tttccgacgc cagccgcgac
      121 aaagcgcgac tcatccgcgc gccgtccata attctcgatt tttcaactaa agtgtgttca
      181 tctgccatct tgccgaagat agtaacaaac gcgcgggaac agtgacaagc gaagcgaaac
      241 caaatagaat aaaatcgcta tggcgcttcc gaaaacttac ccgattttta cgattgccga
      301 atatctcgac tatgagcgct acgccgaatc gcgtcacgaa tttctcgacg gcacggttta
      361 cgcgatggct ggcgaaagcc cgcgccacag cacgatttgt tttaatcttt atgcgacgat
      421 aggcagtcag ctgcgcggca aacgctgtcg cggcttctcg ccgaatatga aaatcgcaag
      481 cagcaacaaa ggcttgtttt cctacccgga tttggcgatt gtctgcggcg agccgcttta
      541 tcacgacgac aagcaggacg ttttgacgaa tccgcgggtg attttcgaag tgctttcgcc
      601 ttcaacccaa agttacgacc gcggcgaaaa gcttctgcgt tatacgaaca ccatcgaaac
      661 attaaccgat tacgttttaa ttgcgcagga cgcgccgctg gtcgagcatt tccaaaaaca
      721 ggcggacgga aactggctga aattcgagat ttccggactc gaatcaatcc tgcatctcgc
      781 cgccgtcgaa tgcgagattc cgctttccga gctttattat ctggtcgatt tttcgtgaaa
      841 ctttatcaat ctttaattcg cccgattctt ttcaaaatgc cgaccgaaac ggcgcacgag
      901 ctcggcatta acgcgctgaa gctgggttta ggcaacgagt ttctgcagaa acgttttgcc
      961 ggaaaatacg cctgcgacga attcggcgag ctggagcgtt ttaatttaaa attcaaaaat
     1021 ccgttcggcg tcgcggcggg ctttgataaa aacgcggtcg tcgccagcca gctctgcgcg
     1081 ctcggattcg gtttcgtcga agtcggaacg gtgactttcg agccgcagaa gggaaatccg
     1141 aaaccgagac tttttcgcct gcccgaagat aaagccttga taaaccgcgc cggatttaac
     1201 aacgaaggga cgccggcggt cgtcgaaaga ttgaaaaacc ggcgtcccga ctgcgttctc
     1261 ggcgtaaaca tcggcaaaaa caaggacgtg gcaaacgagg cggcgacgga aaattatctg
     1321 tcgagctttg atttggcgca cgcggttgcc gattacatcg cggtcaacgt ttccagcccg
     1381 aacacgccaa acctgcgcga gctgcaaaag gcggaaactc tggaagagct tttaggcgct
     1441 ttgcagacga gaaacgcgga attaaatcag ctgaacaaaa aaaatctgcc tcttctcgtc
     1501 aaaatcgcgc ccgatttgag cgaagccgaa atcgaatccg tcaccgacgt ctgtctgcgt
     1561 ttgaatttag acggaattat cgcgaccaac acgaccgttt cgagaaataa tctgcgagcc
     1621 tgcgccgcgg aaatcgagcg aatcggaaac ggcggcttga gcggaaagcc tttaaacgcg
     1681 cgctcgaccg aagttatttc aaaaatccat cgttattcga aaggcaaatt gccggtcgtc
     1741 ggcgtcggcg ggatttttac gccgcaggac gcgtttgaaa aaatcgcggc gggcgcttgt
     1801 ttgatacagg catacacggg ttttgtttat cagggtctgg cttttgcccg cgatattaat
     1861 ttcggtctgg cgaaaatcct gcgcgagaaa ggttttaaat cgctcgacga agcgatcgga
     1921 acgagagcca atctgcgaca ataaaaagaa ttctcactgc gaaacgaggg aaaaaaaact
     1981 ggcgaaagaa atttaaatgt ttttattttt agtgtttttc gcgcgtttc
//
LOCUS       DASWNE010001024         3453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_416623, whole genome shotgun sequence.
ACCESSION   DASWNE010001024 DASWNE010000000
VERSION     DASWNE010001024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416623"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3453
                     /locus_tag="VGB68_19615"
     CDS             <1..>3453
                     /locus_tag="VGB68_19615"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY0051515.1"
                     /translation="YALAGAVAASLLFVLIHFFNNSKITDSSQIAERTLIENNSPATP
                     ADTGAAAIDKEKIVDAYGKLPLHFEPNHGQTDAQVKFLARGQGYALFLTEKEAVLSLQ
                     KRGKTKAQDKRAVVRMQIEGASDAAQSGGLEQTESKSNYFIGNDSAGWQSDVPNYSKV
                     RYEQIYEGVDLVYYGNNQRLEYDFLVRPDADPNQIRLKFDGVKSAEIDKQSGDLLLET
                     EVGTLRQHAPVVYQDIDGERKEIASSYELQNSKLETRNSENKGFTVSFKLAGYDKSKE
                     LVIDPILAYGSYLGGTLFDEGRSIAVDAGGNAYIVGTAASRDFPTTAGTIKPVMLSRE
                     DAPNSYWYDAFVTKVNPTGTAIVFSTYFGGRNGNETGLGVAVDGGGNVLLSGLTMSPD
                     FPLVNAYSPTYNNIDMNFAAKLNSTGSAIIYSTYLGTSTGAGSKIAVNPAIGDAVFAG
                     TTGTANFPTTPGAYKEKLCDAPPSCNGIFYSGSYIVKFAANGSAIYSTLFPNTGINDV
                     ALDADGNAVLGGTAAMQFPATPGAFQTATSGGVEGFVAKLNSAGNALVFGTFLGGGLQ
                     SDRVKGVAVDSTGNVYVTGQTENGGFPTTAGAFDQTFNGGADGFLTKFNPAGSALVYS
                     TFFGGTGKDEPFAIGLGSDNSAFVTGETLSGANFPLKNSLNGANGTIFLTRFNTDATA
                     LVFSSLLGVGGAYDLAVDGANGAYVTGHTTSIPVTPNSFQPMKGEPTSTSSGKDAFVL
                     KIAPTDESVQAYSISGTVTDPTQFGNYQPIVVTVTGTINRSVILPYGNGSGVIPYFFG
                     NLPTGGNYTITVKKTGFLTDPESAAFSNLSANQFADFTILNNEAPVGVITSPAHATNY
                     TSPAAITIQATASDPDGDAITKVDFVAYHNTLGNIPLGTDTTAPYEFTWTNVPVGTWA
                     LHAIPTDSKGLRGVSTPIVHVSVNDPAGLSVSITSPTEGQSFAQGSDVPLAASVSSSV
                     TILEFYDQNNELIGRRTGAPWSTTWRVMNTGNYTITAKVYNSQGQSLTSTPVGIAVNP
                     LNHRIAGRIFDLTANAPIPNITLNLTSSSNPHLSATATTDSNGNYLLTDFGATVNDSV
                     TITPVSSQYTFEPQTRVINYLGYIHRENENFWGTQITQINVAMTSPTAGQTFTAPAAI
                     NLAA"
BASE COUNT          834 a          964 c          925 g          730 t
ORIGIN      
        1 atatgcgctg gcgggcgcgg ttgccgcctc gctgcttttt gttttaatac atttttttaa
       61 taactctaag attaccgatt cctcacaaat cgccgaacgg acattaatcg aaaacaattc
      121 tccggcgacg cccgcagaca ccggcgcggc ggcgattgat aaagaaaaaa tcgtcgacgc
      181 ttacggcaag ctgccgctcc atttcgaacc gaaccacggg caaaccgacg cgcaggtgaa
      241 atttctcgcc cgcgggcagg gctacgcgct ttttctgacc gaaaaggaag ccgttttgtc
      301 tttgcaaaaa cgcggaaaaa ccaaagcgca ggacaagcgc gcagtcgtca gaatgcagat
      361 tgaaggcgca agcgacgcgg cgcaatccgg cggactggag cagaccgaaa gcaaatccaa
      421 ttattttatc ggcaacgatt ccgccgggtg gcaatcggat gtgccgaatt actcgaaagt
      481 cagatacgag cagatttacg agggcgtcga cctcgtctat tacggcaata atcagcggct
      541 cgaatacgac tttctggttc gccccgatgc cgacccgaat caaatcaggc tgaaattcga
      601 cggcgtcaaa tccgccgaaa tcgacaaaca atccggcgat ttattgctgg aaaccgaagt
      661 cggaacgctc cgccagcacg ctcccgtagt ttatcaggac atcgacggcg aaagaaaaga
      721 aatcgccagc tcgtacgagc ttcaaaactc aaaactcgaa actcgaaact ctgaaaacaa
      781 gggctttacg gtttcgttta aactcgccgg ttacgataaa tcaaaagagc tcgtcatcga
      841 cccgattctc gcttacggca gctatctggg cgggacgctg ttcgacgaag gacgctcgat
      901 tgcggtcgat gcgggcggca atgcttacat cgtcgggacg gcggcttcgc gtgattttcc
      961 gacgactgcc ggaacaatca agccggttat gctgtcgcgc gaagatgcgc ctaattccta
     1021 ctggtatgac gcttttgtga cgaaagtaaa tccgaccgga acggcaatcg ttttttcgac
     1081 ttatttcggc ggtcgcaacg gcaacgaaac gggtttgggc gtcgcggttg acggcggcgg
     1141 aaacgttctg ctgagcggct tgacgatgtc tccggatttt ccgctggtca acgcctacag
     1201 cccgacctac aacaatatcg atatgaattt cgccgccaaa ttaaattcga ccggctcggc
     1261 gatcatttat tcgacttatc ttggcacgag cacgggcgcg ggcagcaaaa tcgcggttaa
     1321 tccggcgatc ggcgatgccg ttttcgccgg aaccaccggc acggcgaatt ttccgacgac
     1381 gcccggcgca tacaaagaaa agctttgcga tgcgccgccg tcctgcaacg gaatttttta
     1441 cagcggaagc tatatagtta agttcgccgc caacggcagc gcgatttatt cgacgctctt
     1501 tccgaatacg ggcattaatg atgtcgcgct cgatgccgac ggaaacgctg ttctcggcgg
     1561 aactgcggca atgcagtttc cagcgacgcc cggcgccttt caaaccgcga cgagcggcgg
     1621 cgtcgaaggc tttgtcgcca aattaaattc tgccggaaac gcgcttgttt tcggaacctt
     1681 cctgggcggc ggcttgcagt cagaccgcgt caaaggcgtg gcggtcgatt cgaccggaaa
     1741 cgtatacgtc accggacaaa ccgaaaacgg cggatttccg accacggctg gcgcgtttga
     1801 ccagacgttc aacggcggcg cggacggatt tttaacgaaa ttcaatccgg caggctcggc
     1861 tttagtttat tcgacatttt tcggcggcac gggaaaagac gagcctttcg ccatcggttt
     1921 aggctcggac aacagcgcat tcgtgacggg cgaaacttta agcggcgcaa acttcccgct
     1981 caaaaattcg ctgaacggcg cgaacggcac gattttctta acgcgcttta atacggacgc
     2041 gacggcgctt gttttctcgt cgcttttagg cgtcggcggc gcgtatgatt tggcggttga
     2101 cggcgcgaac ggcgcgtatg ttaccggaca cacgacttcg attccggtaa ctccgaattc
     2161 cttccagccg atgaagggcg agccgaccag caccagttca ggcaaagacg ccttcgtgct
     2221 gaaaatcgcg ccgaccgatg agagcgtgca ggcttacagc atcagcggaa cggtgacgga
     2281 cccgacgcag ttcggcaatt accagccgat tgtcgtgacg gttacaggca cgattaatcg
     2341 ctccgtcatt ctgccttacg gaaacggaag cggcgtgatt ccatactttt tcggcaatct
     2401 gccgacgggc ggaaattaca cgattacggt taagaaaacc ggctttctga ccgacccgga
     2461 aagcgccgct tttagtaact taagcgcgaa tcaattcgcc gattttacga ttctgaataa
     2521 tgaagcgccg gtcggcgtga ttaccagtcc ggcgcacgca accaattaca cgtcgcccgc
     2581 cgcgattacg attcaggcga cggcttccga cccggacggc gacgcgatta caaaagtcga
     2641 ttttgtcgct tatcacaaca cgctcggcaa tattcctctg ggaacggaca cgaccgcgcc
     2701 ttatgaattt acctggacga atgttccggt cggaacctgg gctttgcacg caatcccgac
     2761 cgacagcaag ggcttgcgcg gcgtttcgac gcctatcgtt cacgtttccg tcaatgaccc
     2821 ggcaggcttg agcgtttcga taaccagccc gaccgaaggg caatctttcg cccagggcag
     2881 cgacgttcct ctggcggcga gcgtttcgtc ttcggttacg attctggaat tttacgacca
     2941 gaataatgaa ttaatcggca gacgaaccgg cgcgccgtgg tcgaccacgt ggcgtgtgat
     3001 gaatacggga aattacacga taaccgcgaa agtttataat tcgcaggggc aaagcctgac
     3061 ttcgacgccg gtcggcattg ccgtcaatcc gctaaatcac cgaattgcgg gacgaatctt
     3121 cgacctgacg gcaaacgccc cgattccgaa tatcacgctt aatctgacga gctccagcaa
     3181 tccgcaccta tcggcgacgg cgacgaccga ttcaaacggt aattacctgt tgaccgattt
     3241 tggcgcgacc gtcaatgaca gcgtcaccat tacgccggtt tcgagccaat acactttcga
     3301 gccgcaaacc agagtcatca attatctcgg ctatatacac cgggaaaacg aaaatttctg
     3361 gggaacgcag attacgcaga ttaatgtggc gatgaccagc ccgaccgccg gacagacttt
     3421 caccgcgccc gccgcaatta atttggcggc aaa
//
LOCUS       DASWNE010001025         4480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_528252, whole genome shotgun sequence.
ACCESSION   DASWNE010001025 DASWNE010000000
VERSION     DASWNE010001025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528252"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="VGB68_19620"
     CDS             complement(<1..884)
                     /locus_tag="VGB68_19620"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEY0051516.1"
                     /translation="MWYRLCFSLLALFSIITGSMQTAFAGEEFYETEKASLAEPPVFR
                     LAAEPNIRIGLTTNARSVSITTTDAQLVAVSPEEPNKLLATNRVAVTARAYRPPVIEN
                     YTFEIPNVATREEAETLAKDVREATGETALVSADIKTNNWRVLIGGVRESIEEANEYK
                     AFLSDKGFEEAEMKTEKIQQPSTDAIALSNQLKTNPNPKSEVRSLVKPTGSSKPTADT
                     ANPTIDANLREVIVNGASETARFSSLKPVAFGSLNERLIPVKLNGKAYRGKIEVFVNS
                     RGSLTVVNVVPMEDYLLGV"
     gene            complement(1373..2527)
                     /locus_tag="VGB68_19625"
     CDS             complement(1373..2527)
                     /locus_tag="VGB68_19625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051517.1"
                     /translation="MRFIKLSAAFFTVALLSTACNLFGTKETARTSPYENDAPQAEAV
                     SEADVRYARENLDLRAVGALLEKAGNPEEFEYLLNSEGVNNLDLNRDGEVDYISVSEF
                     EDRYDEERGISLFSRFSPELIQEIATIIFDREGFRDASGARILLTGNEQLYGDDYYYE
                     TNWRDRSASMIDWLFDDERDNYYRSPYYYGNYPDNYESYEAVETPVYLTRIEQIYDND
                     AAPSFIQTSSPTISRVKIKSPYDGRSVSKNFPKPSKPAKEQKELGKNEPVAPEFVPVK
                     KGKMKDFPPRPDERTRGNRNDFEKPRKDNNAKFETRENPNKIRRDSIKPPKVERQSFN
                     QPGPDKVEKQNMKSPKPGNNGKQKGGGDNQGRGNGGGGNKGGGKGGGKKN"
     gene            2655..2834
                     /locus_tag="VGB68_19630"
     CDS             2655..2834
                     /locus_tag="VGB68_19630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051518.1"
                     /translation="MQKKFLPGSPTDDKCRLREEKHPGKAMTIYSYLAGFYAGTPGKT
                     GTPGNKCNETPIMFV"
     gene            2890..3180
                     /locus_tag="VGB68_19635"
     CDS             2890..3180
                     /locus_tag="VGB68_19635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051519.1"
                     /translation="MKKLLTLALAVASLGFAASAAEAKTAEAPSTSITANAVVPQIRI
                     RIGQPRRNRRDYRGNRNYGQWRRNQVRNRRARVVTQTYWRNGRRYTRTVRVY"
     gene            3614..>4480
                     /locus_tag="VGB68_19640"
     CDS             3614..>4480
                     /locus_tag="VGB68_19640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051520.1"
                     /translation="MKSRKLVPIIFLLIISILSFQRAARAQAGALDPNFGTNGLVPNL
                     LIQNQNDYGLSDTALQPDGKIVVAGGITFRYGTHPNWQFQTRFLLVRYKPDGSFDPEF
                     GTGGISAIPFARSASAASVSILPDGKFLAAGWMNGYSADLALARYNPDGSLDTSFGVG
                     GLVVEDFAAFEEESQEYPVKSFHLPDGKILVIGRKSYRSALYPADGIPAYSVFLARYN
                     ADGTPDTTFGRFGKVFPGTPYFCGTADSAVMQPDGKILVSDRVCKPVYLISQLTRYNS
                     DGSVDTGFGHNGT"
BASE COUNT         1037 a         1152 c         1140 g         1151 t
ORIGIN      
        1 acgccgagca gataatcttc catcggcacg acgttgacga ccgtcaggct gccgcgcgaa
       61 ttgacgaaga cctctatttt gccgcgatac gccttgccgt ttaatttgac cggaatcaag
      121 cgctcgttga gcgagccgaa agcgacgggt ttcagcgacg aaaatctcgc cgtctcgctc
      181 gccccgttga cgatgacttc gcgcagattc gcgtcgattg tcggattggc ggtgtcggcg
      241 gtcggtttcg aagagccggt cggtttgacg agactgcgga cttcggattt cgggttcgga
      301 ttcgttttta attgattgga aagcgcaatc gcatcggtcg agggctgttg aatcttttcc
      361 gttttcatct cggcttcttc aaagcccttg tcgctcagaa aagccttgta ttcgttagct
      421 tcttcgatgc tctccctgac gccgccgata aggacgcgcc agttgttcgt ctttatatca
      481 gccgagacga gcgccgtttc gcccgtcgct tcgcgcacgt ctttggctaa ggtctcagcc
      541 tcctcgcgcg tggcgacgtt cggaatctcg aacgtgtaat tttcgataac gggcgggcga
      601 taagcgcgcg ccgtgaccgc tacgcgattg gtcgccagaa gtttgttcgg ctcttccggg
      661 ctgacggcga ccagctgcgc gtcggtcgtc gtaatcgaaa ccgaacgggc gttggtcgtc
      721 agcccgatgc ggatattcgg ctcggcggcg agccggaaaa ccggcggctc ggcgagcgaa
      781 gccttttccg tttcgtaaaa ttcttcgccg gcaaaagccg tctgcatcga acctgtaatg
      841 attgaaaaaa gcgccagcag ggaaaaacaa agacgatacc acataaagac cataatagaa
      901 gtccgcgccg gaaatgtgaa gcagaaagct tcccgattaa tcaaaacggc tttcgcgaca
      961 atagtttgag cgcggaaaag attaaaaaaa accagcggct ttgtttaaaa aaccgctatt
     1021 gaggaaatac gcctattgag aaaataggaa agtcggcgaa ttcaaatctt ttgcagcggt
     1081 cttttcgaaa cgcatcgcga cgctggcgga cacaaattaa atccggcgag tcgcaaaagt
     1141 catctgaacc agcgaatttt tttgaaagcc aatccaatcg gcgagccgcc tccggtcgtg
     1201 ccgggcgcgg aggcaatcat caatccgtcg gtcggctgga aaggaaactc ttttcttatg
     1261 gcgaattaat ctaaaacaat taatttcaca atcgaatcga cggtaatatc cgcttcaacg
     1321 caaacagcgc cgagttttca aaatttttgt gatgcagagc aaatcaggaa aatcaattct
     1381 ttttcccgcc gcctttgccg ccgcctttat tgccgccgcc gccgttgccg cgtccctgat
     1441 tatctcctcc gcctttttgt ttaccgttgt taccgggttt aggcgatttc atattttgtt
     1501 tttcaacctt atccggtccg ggctgattaa agctctgtct ttcgaccttt ggcggtttta
     1561 tcgaatctcg ccggatttta ttcggatttt cccgcgtttc aaattttgca ttattatctt
     1621 ttcgcggttt ctcaaaatcg ttccggtttc ctctcgttcg ttcgtccggg cgaggcggaa
     1681 aatctttcat ttttcctttt ttaacgggga caaattccgg cgcgaccggc tcattttttc
     1741 cgagttcttt ttgttctttt gccggtttgg acggtttagg gaaattttta cttaccgacc
     1801 tgccgtcgta aggcgatttg attttgacgc gcgatatcgt cgggctcgac gtttgtataa
     1861 acgacggcgc ggcatcgttg tcataaatct gctctattct ggtgagataa accggcgttt
     1921 cgaccgcctc ataagactcg taattatccg ggtaattgcc gtagtagtag ggcgagcgat
     1981 agtaattatc gcgttcgtcg tcgaaaagcc agtcgatcat cgacgcgctc cggtcgcgcc
     2041 agttagtctc atagtaataa tcgtcgccgt agagttgttc atttcccgtc aataaaatcc
     2101 tcgcgcccga agcatcgcga aagccttcgc ggtcgaaaat tatcgtcgcg atttcctgga
     2161 tgagctcggg gctgaaacgg ctaaacagcg aaatcccgcg ctcctcgtca taacggtctt
     2221 caaactcgga gacgctgatg taatcgacct cgccgtcgcg gtttaaatcg agattgttaa
     2281 cgccttcgga attaagaaga tattcaaatt cttcggggtt gccggctttt tccagcagag
     2341 cgccgactgc ccgcaagtcc agattttctc tggcatatcg aacgtccgcc tcggaaacgg
     2401 cttccgcctg cggcgcgtcg ttttcgtatg gactcgttcg tgcagtttct ttcgttccaa
     2461 acaaattgca agccgtcgaa agcagcgcga cggtaaaaaa agccgccgat aattttataa
     2521 acctcatttt cactagccct cccttatccg tttatgaccg aatgtttcag gcgaataatg
     2581 ttgttaaacc cgctaatttg tcaggctcaa aacgcttaat ctaaaaaatt agtgtcctgt
     2641 ttgattggtt cgggttgcaa aaaaagttcc tgcccggctc gccgacagat gataaatgcc
     2701 gcttgcgcga ggaaaagcat ccgggaaaag ctatgacgat ttattcgtat ttagccggat
     2761 tctacgccgg cacgcccgga aaaaccggca cgccgggaaa taaatgtaac gaaacgccga
     2821 ttatgtttgt ctagcttaac aggcggaatt tttccgacct gaaattaaat cgaacagggg
     2881 ttattaaaca tgaagaaatt attgacattg gcgctggcgg tcgcgtcctt aggattcgct
     2941 gcttcggcgg cggaagccaa aacggcggaa gcgccttcaa ccagcattac ggcaaacgcc
     3001 gtcgtcccgc agataaggat tagaatcggg caaccgcgcc gcaatcgccg tgattaccgc
     3061 ggcaaccgca actatggtca gtggagacgg aatcaggtga gaaatcgccg cgcccgtgtc
     3121 gtgacgcaga cttactggcg taacggacgc cgttacacac gcacggtaag ggtttattaa
     3181 ttaaattcat tcaatttaaa cgagagaggc gcagtggtta aatactgcgc ctctttcgtt
     3241 taagagcagg atatttttat cgcggttaaa aactcgatgc tcttctaatc cgtcgcgcga
     3301 tttatttcag gctcggtcga aatttaattt accggctgcc cgggaaagga aacgtaataa
     3361 gggaaaaggc tcgcgcggat ttataaaaca aagaattttc gctgccgctt tgaccggaca
     3421 gagaagaaaa ggaatgttta ttttataatc cggcgcgagc cttttttcct gatagaacag
     3481 cgtaataagg cttgagcgtg caagcgcgag ataaatcggt tgttgctctt ttattaaact
     3541 ttgttttatt atatttaaca aggctcgccc tttcttccag cccttaaatt ccagcaaacg
     3601 aggtcatacc aaaatgaaaa gccgcaaact cgtgccgata attttcctgc ttattatttc
     3661 cattctgagc tttcaacgcg ccgcccgggc gcaggcgggc gcgctcgacc cgaatttcgg
     3721 cacgaacggc ttagtgccga atctgcttat tcagaatcag aacgattacg gtctgagcga
     3781 tacggcttta cagccggacg gaaaaatcgt cgtcgccggc ggcattacgt ttcgctacgg
     3841 aacgcatccc aattggcaat tccagacgcg gtttctgctc gtccgctaca aacccgacgg
     3901 cagctttgac ccggagttcg gcacgggcgg catttcggcg attccgttcg ctcgaagcgc
     3961 gagcgcggcg tccgtctcca ttctgccgga cggaaaattt ctagccgccg gctggatgaa
     4021 cggctattcg gcagacctcg cgctcgcccg ttacaacccg gacggcagcc tcgacacttc
     4081 gtttggggtc ggcggcttgg tcgtcgagga tttcgccgct tttgaagagg agagccagga
     4141 atacccggtc aaatcgtttc acctgccaga cggcaagata ctggtcatcg gcagaaaatc
     4201 ctaccgctcc gcgctttatc cggcggacgg tattccggct tattccgttt ttctcgcgcg
     4261 ttataacgcg gacggcacgc cggacacaac gttcgggcga ttcggaaagg tgtttccggg
     4321 aactccgtac ttttgcggga cggcggattc agccgttatg cagccggacg gaaagattct
     4381 cgtctcggac agagtttgta agcccgtcta tctcatctcg caattaacca gatataattc
     4441 tgacggaagc gtcgatacgg gtttcggtca caatggaacc
//
LOCUS       DASWNE010001026         5218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_34274, whole genome shotgun sequence.
ACCESSION   DASWNE010001026 DASWNE010000000
VERSION     DASWNE010001026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5218
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34274"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="VGB68_19645"
     CDS             complement(<1..894)
                     /locus_tag="VGB68_19645"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HEY0051521.1"
                     /translation="MRQKLFIFSGLIFLAFLLVGLNVASYVQKEKVPDNESNPNRSTY
                     NTGATGTRAFFDLLNETGRRPIRWQQAPVELLSARKDTPQTFVVVGHFQNEFTDEELE
                     HLLRWVSGGGKLVVIDREPHRKLLSTTADWSIQAAQKDGVPFGTDPSNRVQMIDKVTA
                     GKPVQPSVYTRNVNAVQPSRFASSVQLEFFGASGEGGSNTGSASPTPKYAVVKTASPT
                     PVFQDDDEEYDEAPPKQTEPGRGSGQESGDGIGAAPTPMSEVETTAQNAPVVHLANKD
                     KTLLVDFPYGSGQIVYLSDPYM"
     gene            complement(978..2033)
                     /locus_tag="VGB68_19650"
     CDS             complement(978..2033)
                     /locus_tag="VGB68_19650"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEY0051522.1"
                     /translation="MRNGKLTRLAKILVFMLAFSVSADAVNLSEYRGKIEEVRDELEF
                     LLNHGESETEAEIRAEERAALAVVRSTLQPTEIVELQGARFEADNGWLFAKLKIFDAE
                     PSLTKRQAILSEVVERLRAVEAKLDELEKQEISNRAKDEDKQKLEEILRRAEYQKPEE
                     KQKTRLERAIDDFLKWLSKLFPASPQIGENESAARSSSFILQMALYAVVLGIIGFLLY
                     RFAPFLRERFFKREKTEKKERVILGETLAENETSHNLFDEAERLAREGNLRAAIRKGY
                     IALLCELSDRKIIGLANHKTNRDYLRDVRKRPELYRNMNALTSNFETVWYGFGKAEAE
                     DWEKFREKYNETVKIRN"
     gene            complement(2078..3175)
                     /locus_tag="VGB68_19655"
     CDS             complement(2078..3175)
                     /locus_tag="VGB68_19655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051523.1"
                     /translation="MNRDASTQTMSLAPLGAGDLIDRAVRLYRSNFWTFVWIASPPII
                     LGTVFTIGWTILGRELFSFGSNRDPDDYIISWFFTGFGNLFIWLVETIAVFSVMGGAS
                     RNFVRHLLFGEPITVRETYRNTWSRLPGLIISSILITILLGTTAIFIFYIGMIIAVLA
                     VIAAVYFFSFSDFLSFTVSLLLVAAIAFGAGWIFFLVASRFAYVPQVMLVEGQGVFSA
                     IGRSANLASGNVKRLYALFIFTLLATYSALLLLYVPLGWYGYLNGVQFFSYDPDAIPA
                     WYTIANQAISQASLILLMPVWMVGLCLLYVDERVRHEGYDIELMAARRLGEIPALSQN
                     YVNPLQPALSMTNLQSSRQEPKSPITTLGLN"
     gene            complement(3263..>5218)
                     /locus_tag="VGB68_19660"
     CDS             complement(3263..>5218)
                     /locus_tag="VGB68_19660"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0051524.1"
                     /translation="ILNKSNQTNVISNLNFGNLFSGDQLLIDHNLALSETHIFNPNLI
                     NEFRFSFIRRNLDFPENDGTTPTTTIGGLVTFGGANNFPQSRISNFYQFSNTLTYVFG
                     KHTFKAGADLRRNIMNNFSGFDIKGTFGFSSLQEYLNNNAATFTQAFSAANFNAKQWQ
                     QFYFAQDDWRVTQSLTLNLGFRYETANVPFGFFGTDDPAQNAALVPTPVKRDNNNFAP
                     IFGFAYSPRFEDGLMGTLFGDGLTVIRGGYRTAYDVLFFNILTVNAGNFPITTSVTQN
                     SVFDVFPILAPPSTSPTFNPLATFVNSPPDMENPESYLYSLSVQREIAKSFVFEAGYT
                     GSRSINQINQLQANPAILTAAQIATVRTTLNANSIPTAQQRRIYPNLGQRVLIAGTAQ
                     STYNAGYVSLNRRFGNGLTFGMAYTRSKLMSNNDESLGVAAITGGSPQIPQDFTNYTP
                     EKSLSAFDRKHRFVAHYLYEVPLPGFLERNFITKSLFGGFQLSGITQYQSGQPFTILT
                     GVDSNGNGQGGDRPNFVPGGTFIPDPVTGNLRTFTAPLIGGAFFVPLGTNGLPLANSL
                     GNGNLGRNTLRAPGFWNTDLSVLKRFVLPWGGEENHRLILRADFLNAFNQDNYGIPVN
                     SMNSVDFGKNLNNWGNRSITLGIKYSF"
BASE COUNT         1269 a         1356 c         1361 g         1232 t
ORIGIN      
        1 catatacggg tcgctcagat aaacgatttg accggagccg tagggaaaat cgaccagcaa
       61 agttttatct ttattggcga gatgaacaac gggcgcgttc tgcgccgtcg tttctacctc
      121 ggacatcggc gtcggcgctg cgccgatacc gtcgccgctc tcttgcccgc ttccgcgccc
      181 tggttccgtt tgcttcggcg gcgcttcatc atattcttcg tcgtcatcct gaaaaaccgg
      241 cgtcgggctc gctgttttca ccactgcgta tttcggtgtc ggtgatgccg agccggtatt
      301 cgatccgccc tcgcccgacg cgccgaaaaa ttccaattgc accgacgagg caaaacgcga
      361 aggctgaacg gcgttgacat ttcgcgtgta aacgctcggc tggacgggtt ttccggctgt
      421 cactttgtcg atcatctgca ctcgattcga cgggtctgtg ccgaacggaa ctccatcttt
      481 ttgagcggct tgaatcgacc agtcggcggt cgtcgaaagc aatttccggt gcggctcgcg
      541 gtcaatcacg accagctttc cgccgcccga aacccagcgc agaagatgtt cgagctcttc
      601 gtcggtaaac tcgttttgaa aatgcccgac gacgacaaaa gtttgcggcg tgtcttttct
      661 cgccgataaa agctcgaccg gcgcttgctg ccagcgtatc ggtcgcctgc ccgtctcgtt
      721 gagcaaatcg aaaaaagcgc gcgtgcccgt cgcgcccgtg ttataagtcg agcgattcgg
      781 gttcgattcg ttatcgggaa ctttttcttt ctggacgtaa gaagcgacat ttagcccgac
      841 cagcaggaat gccagaaaaa tcaaacctga aaatatgaag agtttttgcc gcatcctttt
      901 ttcaattaag aattacaaat tacgaattac gaattggaag aatctttcct ttattcgtaa
      961 ttcttaattc ttaattttta atttctaatc tttaccgtct cgttatattt ctctctgaat
     1021 ttttcccaat cctcggcttc cgccttgccg aagccgtacc agacggtttc gaaattgctg
     1081 gtcagcgcat tcatattccg atacagttcg ggacgcttac gaacgtcgcg cagataatcg
     1141 cggttggttt tatgatttgc cagcccgatg attttccggt cgctcaattc gcacaaaagc
     1201 gcgatgtagc ctttgcgtat cgccgcgcgc agatttccct cgcgcgccag ccgctcggct
     1261 tcgtcgaaaa gattatgcga agtttcgttt tcggcgagcg tttcgcctaa gataacgcgt
     1321 tcttttttct ccgttttctc gcgtttgaaa aatctctcgc gcagaaacgg cgcgaaacga
     1381 taaagcagaa aaccgataat gcccagcacg acggcgtaaa gcgccatctg caaaataaac
     1441 gaggaagatc gcgccgcgct ctcgttttct cctatctgcg gcgaagccgg aaaaagcttc
     1501 gaaagccatt taagaaaatc atctatcgcc ctctccagcc gggtcttctg tttttcttcg
     1561 ggcttttgat attcggcgcg gcgcaggatt tcttcgagct tctgtttgtc ttcgtctttg
     1621 gcgcgattgg aaatttcctg tttttccagc tcgtccagtt ttgcttcgac cgcgcggagc
     1681 cgctcgacga cttcgcttaa aatcgcctgc cgtttcgtta agctcggttc tgcgtcaaaa
     1741 atttttagct ttgcaaaaag ccagccgttg tcggcttcaa acctcgcgcc ctgcaattcg
     1801 acgatttcgg tcggctgcag cgtcgagcga acgaccgcta aagccgcgcg ctcttcggcg
     1861 cgaatctcgg cttcggtctc cgattcgccg tgattgagca aaaattccag ctcgtctctg
     1921 acctcttcga ttttgccgcg atattcggat aaatttacgg cgtcagccga aacggaaaag
     1981 gcgagcataa aaacaagaat ttttgccagt ctggtgagtt ttccgtttct cattcttttc
     2041 ttttttcgtt tctccgtcgg gttgttttta gtatagctta attaagtccc agtgtggtga
     2101 taggtgattt cggctcctgc cgcgagcttt gcagattggt catcgacagc gcgggttgca
     2161 gaggattaac gtagttttgc gataaagccg gaatctcgcc cagccgccgc gccgccatca
     2221 gctcgatgtc ataaccttcg tgcctgacgc gctcatcgac atacagcaga caaagtccga
     2281 ccatccagac cggcatcagc aaaatcaggc tcgcctgtga aatagcctga ttggcaatcg
     2341 tataccaagc cggaatcgca tcggggtcgt agctgaaaaa ctggacgccg ttcagatagc
     2401 cataccagcc gagcggaacg tataaaagca gcagagcgga ataagtcgcc agcagcgtaa
     2461 aaataaacag cgcgtagagg cgcttgacgt ttccgctggc gagattggcg cttctgccga
     2521 ttgccgaaaa aactccctgt ccctcgacca gcatcacctg cggcacatac gcgaaacgcg
     2581 aagcgaccag aaagaaaatc cagcccgcgc cgaaagcgat ggcggcaacc agcagcagcg
     2641 aaacggtaaa agacaaaaaa tcggaaaaac tgaagaaata aaccgccgcg atgaccgcca
     2701 gaaccgcgat tatcattcca atataaaaaa taaaaattgc cgtcgtgccg agcaggatgg
     2761 taatcagaat cgaagagatt attagacccg gcagccgcga ccaggtattg cggtaagtct
     2821 cgcgcacggt tatcggttcg ccgaaaagca ggtgacgcac aaaatttctg gaagcgccgc
     2881 ccatcacgga aaacaccgca atcgtttcga ccagccagat aaacagattt ccgaaaccgg
     2941 taaaaaacca cgagataata taatcgtccg ggtcacggtt tgaaccgaac gaaaataact
     3001 cgcgcccgag aatcgtccag ccgatggtaa acacagtgcc gagaatgatg ggcggcgaag
     3061 caatccagac aaacgtccag aaattactgc ggtagagtcg gacggcgcgg tcaatcaagt
     3121 cgcccgcgcc gagcggagcc agcgacatcg tttgggttga agcgtcacga ttcattgctg
     3181 aaattttaac acgaaatcat aaaaaaagag cgacccttcc aagaaggtcg ctcataaaaa
     3241 ttgaggctga aaacacagtt atttagaaac tgtatttaat gccgagggta atcgagcggt
     3301 tgccccagtt gttcagattt ttgccgaagt caacgctgtt catactgttg acgggaatgc
     3361 cgtagttgtc ctgattgaaa gcgttcagga agtcggcgcg taatatgagg cgatgatttt
     3421 cttcgccgcc ccacggcagg acaaaacgct tcagcacgct taagtccgtg ttccagaaac
     3481 caggcgcgcg caaggtattt ctgccgagat taccgttgcc gaggctgttc gccagcggaa
     3541 gtccgtttgt tccgagcgga acgaagaaag ctccgccgat taaaggcgcg gtaaacgtcc
     3601 gcaggtttcc ggtgaccggg tcgggaatga aagtgccgcc cggcacaaaa ttcggacggt
     3661 cgccgccctg tccgtttccg ttcgaatcaa cgccggtcag aatcgtaaac ggctgaccgg
     3721 attgatactg cgtgatgccc gaaagctgga aaccgccgaa cagcgacttg gtgatgaagt
     3781 ttctttccaa aaagcccgga agcggaactt catataggta gtgcgccaca aagcgatgct
     3841 tgcggtcgaa agccgacaag cttttctcag gcgtgtaatt ggtaaaatcc tgcggaatct
     3901 gcggcgagcc gccggtaatc gcggcgacgc cgagcgattc gtcattgtta ctcatcagct
     3961 tgctgcgagt gtaagccata ccgaacgtca gaccgttgcc gaaccgcctg ttcagcgaga
     4021 cgtaaccggc attgtaagtt gactgcgccg tgcccgcaat cagaacgcgc tgcccgagat
     4081 tcgggtagat acgtctctgc tgggcggtcg gaattgaatt ggcattcagc gtcgtgcgta
     4141 cagtcgcaat ctgcgctgcc gtcaaaatcg ccgggttcgc ctgaagctga ttaatctggt
     4201 tgattgaacg gctgcccgta taaccggctt caaagacgaa acttttggca atctcgcgct
     4261 ggacgctcaa gctgtaaaga taagattcgg gattttccat atccggcggc gagttgacaa
     4321 acgtcgccag cgggttgaag gtcggcgacg tggacggcgg cgccagtatc gggaaaacat
     4381 cgaaaacgct gttttgcgtt actgacgtgg taatcgggaa gttaccggcg ttaaccgtca
     4441 gaatgttgaa aaacaaaacg tcatacgccg tgcgataacc gccgcggata accgtcagac
     4501 cgtcaccgaa aagcgtgccc atcaagccgt cttcaaagcg tgggctgtag gcgaaaccga
     4561 agatgggcgc aaagttgtta ttgtcacgct tgaccggcgt cggaaccagc gcggcatttt
     4621 gcgccgggtc gtccgtgcca aagaagccga acggaacgtt ggcagtttca tagcggaatc
     4681 cgagattcag cgtcaggctc tgcgtcacgc gccagtcgtc ctgcgcaaaa tagaattgct
     4741 gccattgttt ggcattgaaa ttagccgccg aaaacgcctg cgtaaaggtc gccgcattat
     4801 tgttcagata ctcctgcagg ctgctgaaac cgaaggttcc cttaatgtcg aagcctgaga
     4861 aattgttcat aatgttgcgg cgcaggtcgg ctcctgcttt aaacgtgtgt tttccgaaaa
     4921 cgtaagtcaa agtattggaa aactggtaaa aattagagat gcgggactgc gggaaattgt
     4981 ttgcgccgcc gaaagtgacc aaaccgccga tggtcgtcgt cggtgtcgtg ccatcgtttt
     5041 ccggaaaatc cagattgcgg cggataaacg agaaacgaaa ttcattaatc agattcggat
     5101 tgaaaatgtg tgtttcgctc agcgccagat tatggtcaat caaaagctgg tcgccggaga
     5161 acaggttacc gaaattcaga ttgctgataa cgttggtttg atttgatttg ttcaggat
//
LOCUS       DASWNE010001027         1868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_630326, whole genome shotgun sequence.
ACCESSION   DASWNE010001027 DASWNE010000000
VERSION     DASWNE010001027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630326"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VGB68_19665"
     CDS             complement(<1..371)
                     /locus_tag="VGB68_19665"
                     /inference="COORDINATES: protein motif:HMM:NF018436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HEY0051525.1"
                     /translation="MKEETSPTVGEKIEETVKAAEEVIRHPYTKRLARFGFYTKGFLF
                     IVIGILAVLVATGQKGGELADPSGALETIAGARYGKWLLIIFIVGAIGHGLWNVLRGA
                     ADVDNAGGGWQGIFKRGIAVGT"
     gene            complement(433..1179)
                     /locus_tag="VGB68_19670"
     CDS             complement(433..1179)
                     /locus_tag="VGB68_19670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051526.1"
                     /translation="MESNDYASPAQRRKRFVSGLAAAAGCRFFATLVLISVAEFTIND
                     LRFTIYKYVMAIETAGQKPVKETKIKIENQATIQLPKDAEETINKIIKSLPSEHLRGL
                     EKVRIVDFVKHPQLKNTPVPSGDLPGLYHPRAASKGAFLEISAGALLQPTENFTKRWM
                     AKSAFKSNLAGLLFSLVGQHYFLTLRHSVKRTGIEPQVRQYAEKNLKAWSEKQNQNSR
                     RAKLFKPLQPYIQRWAKWLNKKAAAAQKKT"
     gene            1197..>1868
                     /gene="lpxC"
                     /locus_tag="VGB68_19675"
     CDS             1197..>1868
                     /gene="lpxC"
                     /locus_tag="VGB68_19675"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEY0051527.1"
                     /translation="MQTTLAQSVKTGGTGLHTGVEVNLTLKPAPENTGYIFVRTDLDD
                     FEIPASVEYISLCSYATTLLRKGVVLSTCEHLLSALRGCGVDNCFIELDNMEIPIMDG
                     SSENFVELIERAGIVRQPAPRRFLQIREKVVFEQGDKLMSIEPSDKFEIDCMIDFPHP
                     FIRRQSFHFEMLNGSFGKEIASARTFGFATEIESLRKANLALGGSLDNAIVLTPEGML
                     NENPLR"
BASE COUNT          399 a          503 c          485 g          481 t
ORIGIN      
        1 gtgccgacgg cgatgcctct tttgaaaatt ccctgccagc cgccgcccgc gttgtcgacg
       61 tccgccgcgc cgcgcaggac gttccaaagc ccgtgcccga tggcgccgac gataaaaatt
      121 atcaacagcc atttgccgta gcgcgcgccc gcgattgttt ccagcgcgcc cgaagggtcg
      181 gctaattcgc cgcccttttg tcccgtcgcc accagaaccg cgaggattcc gatgacgata
      241 aagagaaagc ctttcgtgta aaaaccgaaa cgcgcgagcc ttttcgtgta aggatgacga
      301 atgacttctt cagccgcttt gaccgtttcc tcgatttttt cgccgaccgt gggtgaggtt
      361 tcttctttca tttttactgt cgcaatagtt gtcagccgga gcggaaaatt ttatatcgaa
      421 attctccgcc gcctaggttt tcttctgcgc cgcggcggct tttttgttca gccatttcgc
      481 ccagcgctgg atatacggct ggagcggttt gaatagcttg gcgcggcggc tgttctgatt
      541 ctgcttttcg ctccacgctt tcagattctt ttccgcatac tggcgcacct gcggctcgat
      601 gccggttcgc ttgaccgaat gccggagcgt caaaaaataa tgctgaccga cgagcgagaa
      661 aagcagcccg gcgagattgc ttttgaaagc ggatttcgcc atccagcgct tggtaaaatt
      721 ttcggtcggc tgcaacagcg cgcccgccga aatttccaga aaagcgccct tgctcgccgc
      781 gcgcggatga tacaatccag gcaaatcgcc gctcggcacg ggcgtatttt ttaattgcgg
      841 gtgtttgacg aaatcgacta tgcgaacttt ttccagcccg cgcaaatgct cggacggcag
      901 gctcttgatg attttattaa tcgtctcttc ggcgtccttc ggaagctgga tggtcgcctg
      961 attttctatt ttaatttttg tttctttaac aggtttctgt cctgcggttt cgattgccat
     1021 aacgtatttg taaattgtaa atcgtaaatc gttaatagta aattcagcca cagatatcaa
     1081 aacgagtgtt gcaaaaaaac gacagcccgc cgccgccgcc agaccgctaa caaagcgttt
     1141 gcggcgctgc gccggactgg cataatcatt gctttccatt tcagcagctt atgcaaatgc
     1201 aaaccactct cgcgcaatcc gtaaaaaccg gcggcacagg cttgcacacg ggcgtcgagg
     1261 tcaatctgac tttaaaaccc gcgccggaaa atacgggcta tattttcgtc cgcacggatt
     1321 tggacgattt tgaaattccc gcttcggtcg aatacatctc tttgtgcagc tatgcgacga
     1381 cgcttttgcg caagggcgtc gtgctttcga cctgcgagca tcttttgtcg gcgctgcgcg
     1441 gctgcggcgt ggataattgc tttatcgagc tggacaatat ggaaatcccg attatggacg
     1501 gctcaagcga aaattttgtc gagttgattg agcgcgccgg aatcgtccgg cagcccgcgc
     1561 cgcgccggtt tttgcagatt cgtgaaaaag tcgttttcga gcagggcgat aaattgatga
     1621 gcatcgagcc gtccgataaa tttgaaatcg attgtatgat tgattttccg cacccgttta
     1681 ttcgccggca atcttttcat tttgaaatgc tgaacggctc gttcggaaaa gaaatcgcct
     1741 cggcgcgcac tttcggcttt gcgacggaaa tcgagagcct gcgaaaggcg aatctggcgc
     1801 tcggcggctc gctcgataac gccatcgtgc tgacgcccga aggaatgctc aacgaaaatc
     1861 cgctgcga
//
LOCUS       DASWNE010001028         2060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_456800, whole genome shotgun sequence.
ACCESSION   DASWNE010001028 DASWNE010000000
VERSION     DASWNE010001028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456800"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="VGB68_19680"
     CDS             <1..694
                     /locus_tag="VGB68_19680"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0051528.1"
                     /translation="DAAINCAAYTDVDGSQTSVEQCYAVNAAGVENLALAAKQFDCGF
                     VTISTDYVFDGANPGFYTQRDTPRPISVYGQAKLEGEIRARNAYARSIIVRSGWIYGE
                     GGTNFLCVIDRLLAEGKTVKVINDAYGTPTFAKDLVKRLREIAGLDLPCIFHVTNSGA
                     GASYESFARKICELKGFDANLLESISAGALKRPAPRPQSSKLACLFSDRLGLPPLQNW
                     EKALEEFLEDKR"
     gene            complement(797..>2060)
                     /locus_tag="VGB68_19685"
     CDS             complement(797..>2060)
                     /locus_tag="VGB68_19685"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEY0051529.1"
                     /translation="ERNAEKEVLTALAAQYQSAQKREQQAQASYLSEVTKANQQGQAA
                     TALTTLTETIETNRSLLNTYIQRQKEQELALSSGRPDNIKITTEAQTPGGPIGPQRNR
                     NIIIAFLVAFAAGIGLAFLIDYLDDSIRTSDDIGRNLGLPTLAMIPHQSLVDKKRGVL
                     PSQNGNGSSTALITLQDNRSAMAEAYRHLRTSLLFSSAGKPPQTILVTSTQPSEGKTT
                     TAINTAVTLAQAGVDVVLLDCDLRRPRLHQHFSLSNATGLTNYLSGERNTENLLKSYP
                     GLPNLKVVTSGPIPPNPAELLSSNEMKNLLQFLKGNFKHVVIDSPPAISFTDAAIMAT
                     LVDGVVLVAMAGKSSIHLMRRFKHRLANLGARIYGVVLNGIKPSALEYGYYGYGHGYT
                     SYYDTPIDDETTPRMEEEETVSSHTTKG"
BASE COUNT          447 a          533 c          577 g          503 t
ORIGIN      
        1 cgacgcggcg ataaactgcg cggcttacac cgacgtcgac ggaagccaaa cgagcgtcga
       61 gcaatgttac gccgtaaatg cagcgggcgt cgaaaatctg gcattggcgg caaagcaatt
      121 cgactgcggt tttgtcacca tttcgaccga ttatgttttt gacggcgcta atcccggttt
      181 ttacacgcag cgcgacacgc cgcgcccgat aagcgtttac gggcaggcaa agctggaagg
      241 cgagattcgc gcgcgaaacg cttacgcgcg ttcaatcatc gtgcgttcgg gatggattta
      301 cggcgaaggc ggaacgaatt ttctgtgcgt gattgaccgt cttttagccg agggaaaaac
      361 cgtcaaggtg attaacgacg cttacggaac gccgactttc gcgaaggatt tggtcaaacg
      421 cctgcgcgag attgccggac tcgatttgcc ctgcattttt cacgtcacga attccggcgc
      481 gggcgcttca tatgaaagct tcgcgcgaaa aatttgcgag ttgaaaggtt tcgatgcgaa
      541 tctgctggaa tcgatttccg ccggcgcatt aaaacgcccc gcgccgcgtc cgcaatcttc
      601 aaaactcgcc tgtctcttca gcgaccgctt aggacttccg ccgctgcaaa actgggaaaa
      661 agcgctcgaa gaatttttag aagacaaaag atgatttaaa agacaaaagt aagaagacaa
      721 aagtaaaaag gcaaagtaag gaaagaaatt atcttttcct tttacttttg ccttttactt
      781 tttacttttg tcttaattaa ccttttgtcg tgtgcgacga aacggtttct tcttcttcca
      841 ttcgcggcgt cgtctcgtcg tcaatcggcg tgtcgtaata gctggtgtag ccgtgaccgt
      901 agccgtaata gccgtattcg agcgcgctcg gcttgatgcc gttcaggacg acgccgtaaa
      961 ttctcgcgcc gaggttggca agacgatgtt taaagcgccg catcaggtga atcgagcttt
     1021 tgcccgccat cgcgaccaga accacgccgt cgacaagcgt cgccataatc gccgcgtcgg
     1081 taaacgaaat cgcgggcggc gagtcgataa ccacgtgttt gaaattgcct tttaaaaact
     1141 gcaaaaggtt tttcatctcg tttgagctta aaagctcagc cggattcggc ggaatcggtc
     1201 cgctggtgac gactttcaga ttcggcaagc ccggataaga cttgagcaga ttttccgtgt
     1261 ttcgctcgcc ggaaaggtaa tttgtcagac cggtcgcgtt gctcagcgaa aaatgctggt
     1321 gcaggcgcgg gcggcgcaaa tcgcagtcga gcagcacgac atcgacaccg gcttgcgcca
     1381 gcgtcaccgc cgtgttgatg gcggtcgtcg ttttgccttc cgaaggctgc gtcgaagtca
     1441 cgagaatcgt ctgcggcggt tttccggcgg acgagaacag cagagaggtt cgcaagtgtc
     1501 gataagcctc agccatcgcc gagcgattgt cctgaagcgt tatcagggcg gttgacgagc
     1561 cgttgccgtt ctggctcggc agaacgccgc gctttttgtc gaccaggctc tggtgcggaa
     1621 tcatcgccag cgtcggcaag ccgagatttc tgccgatatc gtccgacgtg cggatggaat
     1681 cgtcaagata atcaatcaaa aacgccaagc cgatgccagc cgcgaacgcg accagaaagg
     1741 cgatgatgat gtttcgattg cgctgcggtc cgattggtcc gccgggcgtc tgcgcttcgg
     1801 tcgtaatctt gatgttgtcg gggcgaccgc tgctcaaagc taattcctgt tctttctgac
     1861 gctggatgta tgtgttgagc aggcttcgat tcgtttcgat agtctcggtc agcgtggtta
     1921 aggcggtcgc ggcttgtcct tgttgattcg cctttgtaac ctcgctcaag tatgaagctt
     1981 gcgcctgctg ttcgcgcttt tgcgccgatt gatactgtgc cgccaaggcg gtcagaactt
     2041 ccttttccgc gtttctttcc
//
LOCUS       DASWNE010001029         2107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_69558, whole genome shotgun sequence.
ACCESSION   DASWNE010001029 DASWNE010000000
VERSION     DASWNE010001029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69558"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1360)
                     /locus_tag="VGB68_19690"
     CDS             complement(<1..1360)
                     /locus_tag="VGB68_19690"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0051530.1"
                     /translation="MGLFLKIFLWFWLATALVVGVLTFITWSMQSEPFLNRWQHAVAN
                     TMRVYSEIARQIYENEGEKGIENFFRNLKVSSEIKNACLVSEKNRWCQNFGEMPGRQA
                     TAVIDRAFAGGAVEFHILAPEESFAAKKFTTAAGENFVLVLWLERPRPPLPLGVDWKT
                     RLLRILAVILTAGLLCYALARYLVAPILKLRQATRKLADGDLSTRIATKRRDELGQLA
                     RDFDEMAERIETLIASQKRLTRDVSHELRSPLARMNVALELAKQKSNDETASLLERIE
                     TESKRLNEMISQILTLSKLETRAEKIEKRELNLTRLIEQIAADAEFEAESRGKSVRIL
                     RADNCRVLGNDSLLRSAIENVLRNAVRYTKEGTNVEISLKTEGEKAFVTIRDYGAGVA
                     DNELSEMFRPFYRIGEARERKTGGIGLGLAITEQAVRAHEGRISARNAESGDGLIIEL
                     EMP"
     gene            complement(1485..>2107)
                     /locus_tag="VGB68_19695"
     CDS             complement(1485..>2107)
                     /locus_tag="VGB68_19695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY0051531.1"
                     /translation="GFSVSAVHDGAEGLQRAASDEFDLVTLDVMLPGMDGFDVLHELR
                     RKSQIPVLMLTARGDDMERIVGLEIGADDYLAKPFNPRELAARLRAILRRTQNLPEEQ
                     LPPEKIRIDDVEVSLSARSARREGEDLNLTSVEFDLLAELLKNAGKVIKKEDLNQKVL
                     GRRLSPFDRSLDMHISNLRKKLGSRENGGDERIKTIRSVGYIYTIV"
BASE COUNT          446 a          617 c          523 g          521 t
ORIGIN      
        1 aaggcatttc aagctcgata atcaaaccgt cgccgctttc ggcgtttctt gccgaaatcc
       61 tgccttcgtg cgcccgaacc gcctgttcgg taatcgccag ccccagaccg attccgcccg
      121 tctttctttc gcgcgcctcg ccgatacggt aaaacgggcg aaacatttcc gagagttcgt
      181 tatcggcgac gcccgcgccg taatcgcgaa tcgtcacaaa tgctttttcg ccttcggttt
      241 tcagcgaaat ttccacgttc gtgccttctt tcgtgtagcg cacggcgttg cgcagaacgt
      301 tttcaatcgc gcttcgcaaa agactgtcgt tgccgagcac gcggcagttg tcggcgcgca
      361 gaatcctgac cgacttgcct ctgctttcgg cttcaaattc cgcgtctgcg gcgatttgct
      421 cgattaagcg cgttaaattc agctcgcgtt tttcgatttt ttccgcgcgc gtttccagct
      481 tggaaagcgt cagaatctgc gaaatcatct cgttcagccg tttcgattcg gtttcgattc
      541 tttccagcaa agacgcggtc tcgtcgtttg atttttgttt ggcgagttcg agcgcgacgt
      601 tcattcgcgc gagcggcgag cgcaattcgt gcgaaacgtc tcgcgtcagc cgtttttgcg
      661 aggcgatgag cgtttcgatg cgctccgcca tctcgtcaaa atcgcgcgcc agttgtccga
      721 gctcgtcgcg tcttttcgtg gcgatgcgcg tgcttaaatc gccgtccgcc agttttctgg
      781 tcgcctgccg gagcttcaaa atcggcgcga ccagataacg cgcgagcgcg taacaaagca
      841 agcccgccgt caaaattacg gctaaaattc tgagcaggcg cgttttccag tcgacgccga
      901 gcggcagcgg cgggcgcggt cgctcgagcc acaaaaccag aacgaaattt tcgccggcgg
      961 cggtcgtgaa ttttttcgcc gcgaaacttt cttcaggcgc taaaatatgg aattcgaccg
     1021 cgccgccggc aaaagcccgg tcaatcaccg ccgtcgcctg cctgcccggc atttcgccga
     1081 aattttgaca ccaacggttt ttttcgctca ccagacacgc atttttgatt tcgctggaaa
     1141 ccttgagatt ccgaaaaaaa ttctcgattc ccttttcgcc ttcattttca taaatctgcc
     1201 gggcgatttc cgagtaaacc cgcatcgtat tggcgacggc gtgctgccac cgattcagaa
     1261 acggctcgga ctgcatagac cacgtgataa aagtcaaaac gccgacgacc agcgccgtcg
     1321 ccagccagaa ccagagaaaa attttcagaa acaaacccat ttgatatttc cggatttcga
     1381 ttgtgtgatt tgcgattttt tattgttctc gggtcttcgt cgaaaatttg ctcgatgttc
     1441 ggcagggaaa atcgaagacc gaagagcgtt ttaaaaatct caaatcacac aatcgtataa
     1501 atataaccga ctgagcgaat cgtcttgatg cgctcgtcgc cgccattctc acgagagccg
     1561 agtttttttc gcaggttcga gatgtgcata tcgaggctgc ggtcaaaagg cgaaaggcgg
     1621 cgacctaaaa ctttttggtt caaatcttct tttttaataa ctttgcccgc gtttttcaaa
     1681 agctccgcca gaaggtcgaa ttcgaccgaa gtcagattca aatcctcgcc ttcgcgccgc
     1741 gccgagcgcg ccgacagcga aacctcgacg tcgtcaatcc tgattttttc cggcgggagc
     1801 tgttcttccg gcaaattctg cgttcttctt aaaatcgcgc gcagacgcgc cgccagctcg
     1861 cgcgggttga aaggctttgc cagataatcg tccgcgccga tttccagccc gacgatgcgc
     1921 tccatatcgt cgccgcgcgc cgtcagcatc aaaaccggaa tctgtgattt tcggcggagc
     1981 tcgtgcaaaa cgtcgaagcc gtccattccg ggcagcatca cgtcgagagt taccaaatca
     2041 aactcgtccg aagccgcccg ctgcaatcct tccgccccgt cgtgaacggc gctcacggaa
     2101 aaaccct
//
LOCUS       DASWNE010001030         2302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_105616, whole genome shotgun sequence.
ACCESSION   DASWNE010001030 DASWNE010000000
VERSION     DASWNE010001030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105616"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..2084)
                     /locus_tag="VGB68_19700"
     CDS             complement(108..2084)
                     /locus_tag="VGB68_19700"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEY0051532.1"
                     /translation="MTENSAALFSETYLNPVYPHSFPDPFVLKFRGEYFAYCTDFWRD
                     GKVFGVLRSRDLVNWQEIGGAMQPLETGAPFYWAPEVTYHNGKFYLYYSVGNETLMEL
                     RVAVSDRPEGGFVDSGHRLSNEDFAIDAHVFEDAGGSRYLFYATDFLEHTHIGTGTVV
                     DRMIDFFTLEGKPRPVTRARYDWQIYDPQRREKGGVRWHTVEGAFVFKRKGVYYEMFS
                     GGNWQNVTYGVSFAVTDDIEKDEEWTQFSDGEKVFPILRTLPELVVGPGHNCAVRGAN
                     NRELYAVYHRWTDGGRVLAIDRMDFAGGARIFISGATHTPQPAPFKPQVQDFFDEFST
                     ADWKSVSGAWRVQNNKIISEFTGKSEFVCKAEAKSFLCEIWLKTFENAGGVFGICLKN
                     GETDVFRFQLAPDEKRASAVWFENGVEQIEDFALAEDFDFQAFHLLRVEIDWLWLKVS
                     LDENTVRCVKILENFAPQISLLTERSTAAFSGFALTEGFEDLFEAANFEKRGWRKISA
                     NGAARVEDKNLIITSREKNETILCKETAADDFELTVNFRLAEVFDENFRVGFYPAFAE
                     NAEKRFFAIEKTGENWILKAAGDGEPQAFRLPKNFSPDVFHQFRFLKIKDKIVLQFEG
                     ETLGIVEAPPDSGKIAVSVQNASVVFDMVRFTVL"
     gene            complement(2111..>2302)
                     /locus_tag="VGB68_19705"
     CDS             complement(2111..>2302)
                     /locus_tag="VGB68_19705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051533.1"
                     /translation="VIEEINDFTVRECPAVFLLEVETASQDSVEEIFYISSGASEVQV
                     LAYSKTIGGNQNSALSLAA"
BASE COUNT          585 a          617 c          547 g          553 t
ORIGIN      
        1 ccaagttgct agttaaaaac ttttatcgtc aacggcaaag agattttaag aaagaatgaa
       61 accgctgatt ttctgcgtta atacgcgttt ccctgtaaat aaatttttta caaaacagta
      121 aagcgcacca tatcgaagac gaccgaggca ttttggacgg atacagcgat tttgcctgaa
      181 tcgggcggcg cttcgacgat gccgagagtt tccccttcaa actgtaaaac gattttatct
      241 ttgattttca gaaaacgaaa ttgatgaaaa acgtcgggcg agaaattttt cggcaagcga
      301 aatgcttgcg gctcgccgtc gcccgcggct tttaaaatcc agttttcgcc ggttttttca
      361 atcgcgaaaa aacgtttttc agcgttttcc gcaaacgccg gataaaagcc gacccgaaaa
      421 ttttcgtcaa aaacttccgc caggcgaaaa tttaccgtca actcaaaatc gtccgccgcc
      481 gtttccttgc agagaatcgt ttcgtttttc tcccggctgg tgattatcaa attcttatct
      541 tcgacgcgcg ccgcgccgtt cgccgaaatt ttacgccagc ctcttttttc aaaattcgcg
      601 gcttcaaaca aatcttcaaa gccttcggtc agcgcaaaac cggaaaaagc cgccgtcgag
      661 cgctcggtca gaagggaaat ctgcggcgcg aaattttcta aaattttcac gcatcgaacc
      721 gtattttcgt cgagcgagac tttcagccag agccaatcga tttcgacgcg caaaagatga
      781 aaggcttgaa agtcaaaatc ttcggcaagc gcgaaatctt cgatttgttc gacgccgttt
      841 tcaaaccaga cggcgctcgc ccgtttttcg tcgggcgcaa gctgaaatct aaaaacgtcc
      901 gtttcgccgt tctttaaaca aataccgaaa acgccgccgg cattttcaaa cgttttcagc
      961 cagatttcgc ataaaaagct tttcgcctcg gctttacaga caaactcgct tttgcccgta
     1021 aattcagaaa ttattttatt gttttgcacg cgccatgcgc cggaaacgga tttccagtcc
     1081 gccgtcgaaa attcatcgaa aaaatcctga acctgcggct tgaaaggcgc gggctgcggc
     1141 gtgtgcgtcg cgccgctgat aaaaattctc gcgccgcccg cgaaatccat acggtcgatc
     1201 gccagcactc tgccgccgtc cgtccagcga tgataaacgg cgtaaagctc ccggttattc
     1261 gcgccgcgaa cggcgcagtt atgtccgggt ccgacgacca gctccggcaa ggttcgcaaa
     1321 atcggaaaga ccttttcgcc gtcggaaaac tgcgtccatt cttcgtcttt ttcgatatcg
     1381 tcggtcacgg caaagctcac gccgtaagtg acgttctgcc agttgccgcc gctaaacatc
     1441 tcgtaataaa cgccttttcg cttaaagaca aacgcgcctt cgaccgtgtg ccagcgaacg
     1501 ccgccttttt cccgtcgctg cgggtcgtaa atttgccaat cgtatctggc gcgcgtgacg
     1561 ggtctgggct ttccttcgag cgtgaaaaaa tcaatcatcc ggtcgacgac cgtgcccgtg
     1621 ccgatatgcg tgtgctccag aaaatcggtc gcgtaaaaaa gatagcgact tccgccggcg
     1681 tcttcgaaaa cgtgcgcgtc gatggcgaaa tcttcgttcg agagtcgatg cccgctatcg
     1741 acgaaaccgc cttcggggcg gtcggaaacc gccacgcgca gctccatcag agtctcgttg
     1801 ccgacgctgt aataaagata aaatttgccg ttgtgataag taacttccgg cgcccagtaa
     1861 aacggcgcgc ccgtttcgag aggctgcatc gcgccgccga tttcctgcca gttgacgagg
     1921 tcgcgcgagc gcaaaacgcc gaatactttg ccgtcgcgcc agaaatcggt gcagtaggca
     1981 aaatattcgc cgcgaaattt taagacgaaa gggtcgggaa acgaatgtgg atagaccggg
     2041 tttagatacg tttcggaaaa aagggcggcg ctattttcgg tcattcaaaa aaaaatcgtt
     2101 caaattaaat ttaagcggcg agcgacaatg ccgagttttg atttccgccg attgttttcg
     2161 aataagcgag aacctgaacc tcgctcgcgc cggacgaaat gtaaaagatt tcttcgaccg
     2221 aatcctgcga agccgtctcg acttccagca gaaacaccgc cggacactca cgcacggtaa
     2281 aatcgttgat ttcctcgatt ac
//
LOCUS       DASWNE010001031         7803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_281956, whole genome shotgun sequence.
ACCESSION   DASWNE010001031 DASWNE010000000
VERSION     DASWNE010001031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7803
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281956"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VGB68_19710"
     CDS             complement(<1..243)
                     /locus_tag="VGB68_19710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051534.1"
                     /translation="MNDRLPKTFKVAAVWALFLLSVLGFYNACQTNSVSLSGDQDASL
                     DNFADKYAASHDASASGDLIASPIDQINLQTLNQYQL"
     gene            complement(250..4275)
                     /locus_tag="VGB68_19715"
     CDS             complement(250..4275)
                     /locus_tag="VGB68_19715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051535.1"
                     /translation="MSKEQANFTDKYIYGILWLLWVATFLLGAVLITYNFLTQNRYLK
                     IDAILIDFDRQAGPLTIGGILPEKNDEESFAERSINDCLGGNAATHESDIILPTQLIP
                     LNIGKLEYNNGKPRLELNNCIDYDGANDKLLNSVAYPKISSGGKRILKSTIPFETLSG
                     EGLTFIYPFRENQYMKIQLEAVGGKKFKLRIIPNPNHLRYVFRDDDDGQPEQAAARIN
                     LVYGRELSHKSIPDCERFDVILFHYNAPAPQSKIISVNKESASVDNQPLTRDYLLDGS
                     IYLDYSPRAGIGILITFLLVGLAFLASFFIPWYRIIEGWKSYYRFTKEGVKGSTYIFL
                     FIPVLALAVFYNVGCILLSHYYFHFERNDYFFKNFTSFDYTNTGVVLLLVFLFPFFGE
                     SNFFRHRVMRFIENWGDLIFWGFLVIGSILVYLSVNNQRVWIFPSHVVVRVFIIVFYF
                     LVGIALIGWDKFKMGITLAVLCLLCFVTKDFGFAFLSIITFLVLVLFNYRLKKETGWK
                     VGFILMPVLFMTVFFVAPMFFSGGDYRSERFQLVYTDYTDLTSKNSIGIENANKETTD
                     KQKIILHEAFTSNFRGYWWNLRIPPEFKTVIWSDYVLLYAFLYFGGFWASLLVIFPLL
                     VLIIYLFKIGKIFYHDLPAQTEYSPHRDVLSKVTGFVLFSYATQMLYMLLAMFRVVPL
                     TGQSIPFLSLSRVENFCIMFFVILIYFIILSPKSPPPITQGAPPVNLLNTKKGFEGLK
                     THFLNTLTGQKFWLGCLSIVVLCVLGWLCNTVPNYAPNSRYIHRNSEKEAIFNHVLRE
                     LSRRDAENTTGKIDLNNVINNVETKNLVGFFDKATDEVTNRWRLRKFIDSYWSGRSFH
                     NARLLQTELAKFYYGRESKLSPAEDTFVYEDTKPTVNREKFLARLTYKQRQRFERKLN
                     EPKIKVVRNGNDFYINFNSAAGAVVPNTLEKVRTGNPPAGGEPEILLDENSAMYTKRI
                     DGDERLQFCNADLYGTLPAVSITDAERLKNKSLTIDERLQALSYSAINSYCRSNRIMG
                     GGIVVLDKEGNIKALASYPSLSPNALPNDRESFIAEHRRGAKQLHLFDGAPSVPLTAY
                     PPASTWKMILAGAAVLVDSSTCNNELAQNLIPSNNDYFENLMTRMVSDQNASRFRERL
                     ENDFDVYLRGNQNPSNLFDASVIARMEDLKNNTKKPGEEKGTREKVAIGHGDCNVSLI
                     ALVQSLARIVTGRKVKPNIFTEIGAEKRELERIEGSEVFSCYKDILNRTSLGGTADVL
                     READYGGLKIFPREKRMYAKTGTYGEQDKGIQQTYIVFGTENYFFGVILNEKPSPAKH
                     AKNLAKDFLPYVRAYCPEYLE"
     gene            complement(4268..6343)
                     /locus_tag="VGB68_19720"
     CDS             complement(4268..6343)
                     /locus_tag="VGB68_19720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051536.1"
                     /translation="MNLIIPTLVFVKSPILLPPSTVETDGDGASASENADAVMNTDAN
                     INTRSDAANAARLLSQLTPPPRPLPAFSDSSIDHLPELSLTHVRNFYDIVKNRRNELD
                     FSKLRIWLVMENIFSNHQPVFSEDLEALREQIDAYLNIPTVEINILALVKNRMRVSDQ
                     LQISLQKAARENIADYILLLNNSNLDWTKSGNVVQLTDEEFYNYGLQMIEYIHFANIM
                     LDDVAFPFNSLNKRRFYFTISSSFKDFFAAGNSQNKLSAPVKEQDEIFRFPDAVRDCL
                     ESFFKEAFKFLQKINQADVFHFLGHRQQRDKARQSIFRALQSEHKTRDDLLNSLYREL
                     YEDFFKNIEQIFTLLTALAVFETGLLRRFKKIKDFIASSNDLEKSKEILPEIINNEVI
                     KKINDAREARLRHLRLEPDRSERDSWQSETESLWQYQFNKNRHAELRRVFKRNLEIIK
                     HPEKAPPPGITAVAAEDFFKPPPIEYKEGTKAKDKSKPEDLIKALRHILKMVLKSAFE
                     NPYMTVQKKFLILDKNRIKDAESLDDLNDAYLIYKQLEKFKDFLSAEQIDKNKFLEEA
                     KRNFEALTRDYLSRAEMTDYTAEIMTRDVADLLYSALNHSKRNQAAARRPTPGFSEKK
                     IEELPAFFRAPSGFSSSAAPVLSVIFTGEETFSAKENLRLIQNINHQDQKFSLFGPWD
                     EQTCFVTNE"
     gene            complement(6349..>7803)
                     /locus_tag="VGB68_19725"
     CDS             complement(6349..>7803)
                     /locus_tag="VGB68_19725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051537.1"
                     /translation="EMSRLQDAGAMGLSAPLTDRQKAEITHRLNRKGIINQLFEHPNQ
                     SNDERFEHILKEIRKVIGEVLGSPAASSSEQTALKENNKFSRTIWLSDTARKQIELGT
                     KVYKTGSLMVQAVFGISQYWFELFTKGNQGTYVADYYGRHSAEQNYYYLPHNDRRFCH
                     TTDADFLQTHDKISDFLKKRENHWRHLYAKEIILTDSRTEKILAYGYLNRNQKSAPIY
                     LKRDDGDSFSIKLVINLSLDEEHFEDRNVEQIYNLTTALAYPLKIVLVLTRDENLYKC
                     EDSSLLSLQTPPLTTPQSPQPENGNSQPPVSGEHESAVVVLPVAVTTATLPIPDALSA
                     SETGEIENFLNAHEGFELFSELKNSLQEAKARLVVELRNNCFVSDHNKDKVIERFLLE
                     MLDAIIEQDINQKIEDEHQRGELISRLKECLQKIGSNYDYLRQHQTERLDEDILLMSR
                     IKEELVKWIRRANQKQIEAEQRKNKRRISGAQPL"
BASE COUNT         1966 a         1827 c         1837 g         2173 t
ORIGIN      
        1 caattgatat tgattgagag tctgcaagtt gatttggtca atcgggctgg cgattaaatc
       61 gccggacgcc gaagcgtcgt gcgaagccgc atatttatca gcgaaattgt cgagcgaagc
      121 gtcctggtct cccgaaagag aaacgctgtt agtttgacag gcattgtaaa aacccagcac
      181 cgacagcaaa aacagcgccc agacggcagc gaccttaaat gttttcggca atctatcgtt
      241 cataattttt tattctaaat actcaggaca atacgcccgg acatacggaa gaaaatcctt
      301 ggctaaattt ttcgcgtgtt tggcgggcga aggtttttca tttaaaatca cgccgaaaaa
      361 gtaattttcc gtgccgaaaa cgatataggt ttgctgaatt cccttgtcct gctcgccata
      421 cgttccggtt ttggcataca ttctcttttc gcgcggaaaa atcttcaaac cgccgtaatc
      481 ggcttcccga agcacgtcgg cggtgccgcc caaagaagtt ctattcagaa tatccttata
      541 acaactgaaa acttcgcttc cttctattct ctccaactcc cttttttccg cgccgatttc
      601 cgtaaaaatg ttaggcttta cttttcttcc ggtgacgatg cgcgccagac tctggacgag
      661 cgcaatcagc gaaacgttgc aatcgccgtg tcctatcgcg actttttctc tggttccttt
      721 ctcttcgccc ggttttttgg tgttgttttt taaatcttcc attctcgcga tgaccgacgc
      781 gtcaaacaga tttgaagggt tctgattacc gcgcaggtaa acgtcaaagt cgttttccaa
      841 acgctcgcgg aaacgagaag cgttctggtc gctgaccatt cgggtcatta aattttcaaa
      901 ataatcattg ttcgacggga taagattttg ggcaagctcg ttattgcagg tggaactatc
      961 gaccagaacg gcggcgccgg ctaaaatcat tttccaggtc gaagctggcg gataagcggt
     1021 cagcggcacg ctgggagcgc cgtcgaaaag atgaagctgt ttcgctcctc tccgatgttc
     1081 cgctataaag ctttcacggt cattcggcaa agcgttcggc gagagactcg gatacgaggc
     1141 gagggcttta atatttcctt ccttatccaa taccactatt ccgccgccca ttattctgtt
     1201 actgcggcag taagaattta tcgcgctata ggaaagcgcc tgcaatcgct cgtcgatggt
     1261 taagcttttg tttttcaagc gttccgcatc ggtaatggaa acggcgggaa gtgtgccgta
     1321 aaggtcggcg ttgcagaatt gcagccgctc atcgccgtcg attcttttcg tatacattgc
     1381 gctgttttcg tccagcaaaa tctccggctc accgccagcg ggcggattgc ctgtgcgaac
     1441 cttctccaaa gtattcggga caacggcgcc ggcggcggaa ttaaaattta tgtagaaatc
     1501 gttaccgttt ctgacaactt ttatcttcgg ctcgttgagc tttcgttcaa atctctgtct
     1561 ttgtttataa gtcagccggg caaggaattt ttcccggtta acggtcggtt tggtgtcttc
     1621 ataaacgaaa gtatcttccg ccgggctgag cttcgattcg cgcccgtaat aaaacttggc
     1681 aagctcagtc tgcagaagac gcgcgttgtg gaaggaacgc ccgctccagt aagaatcgat
     1741 aaatttgcga agcctccagc gattcgtgac ttcgtctgtc gctttatcga aaaacccgac
     1801 cagatttttc gtttcgacgt tgtttatcac gttgttcaag tcaatcttgc ccgtcgtgtt
     1861 ctctgcgtcg cgtcggctca gctctcgcag gacgtgattg aagatggctt ctttttcgct
     1921 gtttctgtga atgtatctgg aattcggggc gtaattcggg actgtgttgc acagccatcc
     1981 caagacgcag agcacaacaa tggacaggca gcccagccaa aacttctgac cggtaagggt
     2041 atttaaaaaa tgtgttttga gtccttcaaa accttttttg gtgttgagta aatttacggg
     2101 cggagctccc tgcgtaatcg gcggcggaga tttgggtgaa agaataataa aatagatcag
     2161 aataacaaaa aacattatgc agaagttttc aactctcgaa agcgacaaaa acggaatgga
     2221 ctgtccggtt aaagggacga cgcggaacat cgccagcagc atatacagca tctgcgtcgc
     2281 gtagctgaat aaaacaaagc cggtcacctt ggacaacaca tctctatgcg gagaatattc
     2341 ggtctgcgcc ggcaaatcgt gataaaagat tttaccgatt ttaaacaaat aaataatcag
     2401 caccagcaaa gggaaaataa cgaggagcga agcccagaag ccgccgaaat ataaaaacgc
     2461 gtaaagcaga acgtaatcgc tccagataac ggttttaaat tccggcggaa ttcttaaatt
     2521 ccaccaatag ccccggaagt ttgaagtaaa ggcttcgtgc aaaattattt tttgcttgtc
     2581 ggtcgtttcc ttgttggcat tctctatccc gatagaattt tttgaagtca ggtctgtgta
     2641 atccgtatag accagctgga agcgttcaga ccggtaatcg ccgccggaga agaacatagg
     2701 agccacaaaa aaaacagtca tgaataagac cggcatcaga ataaaaccga ctttccagcc
     2761 tgtctctttc ttcaacctgt agttgaataa aaccagcacc agaaacgtaa ttatagacag
     2821 aaaggcaaaa ccgaagtcct tggtgacaaa acacaaaaga cacaaaaccg ccagcgttat
     2881 gcccatcttg aatttgtccc agccaatcag ggcgatgccg accagaaaat aaaagacaat
     2941 aataaatacc ctgacgacta cgtggctcgg aaatatccag acgcgctgat tattgacgct
     3001 gagatacact aaaatcgagc cgataactag aaatccccag aagattaaat cgccccagtt
     3061 ctcgataaat ctcatcacgc ggtgtcggaa gaaattgctt tccccaaaaa acggaaataa
     3121 gaaaactaat aataaaacca cgcccgtgtt cgtataatcg aagctcgtaa agtttttgaa
     3181 aaaataatcg tttctctcga aatgaaaata ataatgcgaa agcaggatac agccgacatt
     3241 ataaaaaacg gctaaagcga gcaccggaat aaaaaggaag atataagtgc ttcctttgac
     3301 gccttctttg gtaaatcggt agtaggattt ccagccttca attatgcgat accagggaat
     3361 aaaaaaagaa gccagaaatg ccaagccgac aagcaggaaa gttatcaaaa tcccaatgcc
     3421 cgcccgcggc gagtaatcaa gataaatgct gccgtccaaa agatagtcgc gcgtcagagg
     3481 ttgattatcg accgaggcgc tttctttatt taccgagatt attttgcttt gcggcgcggg
     3541 cgcgttgtaa tgaaaaagaa taacgtcaaa gcgctcgcag tcgggaatgc ttttatgcga
     3601 cagctctctt ccataaacga gattaatcct ggcggctgcc tgttcgggct gcccgtcgtc
     3661 atcgtcgcga aatacatatc gcagatgatt cggattagga attattcgga gcttgaattt
     3721 ctttccgccg acggcttcca gctgtatttt catatactgg ttttcccgga aaggataaat
     3781 gaaggtcagc ccttcgccgc ttaaagtctc aaaaggaatc gtgcttttga gaatcctttt
     3841 tccgccgctg gaaattttcg ggtaagcgac gctgttcagg agcttatcat tagcgccgtc
     3901 ataatcgata caattattca attccagccg cggcttgccg ttattatatt ccagcttccc
     3961 gatgttcaac ggaatcagct gcgtcggcag aatgatgtcg ctttcatgcg tcgccgcatt
     4021 tcctccgaga cagtcattga tactgcgctc agcgaaagat tcctcgtcat tcttctccgg
     4081 aagtatgccg ccaatcgtca atggtcccgc ttgccggtca aaatcaatca ggattgcgtc
     4141 gattttcaaa taacggtttt gcgtcaaaaa attgtaagta atcaggacag cgccgagcag
     4201 aaaagtcgca acccagagaa gccacagaat tccatagata tatttatcgg taaaatttgc
     4261 ttgttcttta ctcattagtc acaaaacagg tctgctcgtc ccatggaccg aacagcgaaa
     4321 atttctggtc ttgatggttt atattttgta taagccgcaa attttccttc gcggaaaacg
     4381 tttcttcgcc ggtaaaaatt accgataaaa ccggcgcagc cgagcttgaa aaacccgaag
     4441 gcgcgcggaa aaatgccggc agctcctcta tttttttctc tgaaaagccg ggcgtcggac
     4501 ggcgcgcggc ggcttgattt cttttactgt gattgagcgc ggaatacagc aaatcggcga
     4561 catcgcgcgt catgatttcc gcggtgtaat ccgtcatctc cgcgcggctc agatagtctc
     4621 tcgtcagcgc ctcgaaattt cttttggctt cttccagaaa cttgtttttg tctatctgct
     4681 cggcgctcag aaaatccttg aatttttcca attgcttgta aatcaggtaa gcgtcgttta
     4741 aatcgtccag tgattcggcg tccttgattc gatttttatc gagaatcaaa aattttttct
     4801 gcacggtcat ataaggattt tcaaaggcgc tttttaaaac catttttagt atgtgccgta
     4861 aggctttgat taagtcttcg ggtttggatt tatcttttgc cttggttcct tctttatatt
     4921 caattggcgg gggcttaaaa aaatcttcag ccgctacggc ggtgatgccc ggcggaggcg
     4981 ctttttcggg atgcttgata atttccagat ttcttttaaa aacgcgccgg agctccgcgt
     5041 gcctgttttt gttgaattga tactgccaga ggctttccgt ttctgattgc cacgagtctc
     5101 tttccgaccg gtcgggttcg agtctgaggt gacgcagtct cgcctcgcgg gcgtcgttta
     5161 ttttctttat gacttcattg tttataatct ccggcaaaat ctccttcgat ttttcaaggt
     5221 cgttggatga agcgataaag tcttttattt ttttaaaccg cctgagcaag ccggtttcaa
     5281 aaacagccag cgcggtcagc agcgtaaaga tttgctcgat gtttttgaaa aaatcctcat
     5341 acaattcacg gtagagggaa tttaacaaat cgtcacgggt tttatgctcg gattgcagcg
     5401 ctcgaaaaat gctctgccgt gctttatctc tctgctgcct gtgcccgaga aaatgaaaca
     5461 cgtccgcctg atttatcttc tgaagaaatt tgaaggcttc cttgaaaaag ctctccaaac
     5521 agtccctgac cgcgtcggga aaacggaaaa tctcgtcttg ctccttaaca ggcgcgctca
     5581 gcttgttctg agaattaccg gcggcgaaaa aatccttgaa gctgctggaa attgtaaagt
     5641 aaaaccggcg cttattcagc gagttaaaag gaaacgccac gtcgtcgagc ataatgttgg
     5701 cgaaatgtat gtattcaatc atctgcaagc cgtagttata aaattcctcg tcggttagct
     5761 gaacgacgtt gccggacttc gtccagtcca gattggaatt gttcagcagc agaatataat
     5821 cagcgatatt ttcgcgggcg gctttttgta aggaaatttg cagctggtcg ctgacgcgca
     5881 tccggttttt gaccagcgcg agaatattta tttccacggt cggaatattc agataggcgt
     5941 ctatctgttc tcttaaagcc tctaaatctt cgctaaaaac gggctgatga ttgctgaaaa
     6001 tattttccat gacgagccag atgcggagtt tggaaaaatc cagctcgttg cgccggtttt
     6061 ttacgatgtc gtaaaaattt ctgacgtgcg tcagcgataa ctccggcaaa tggtcaatcg
     6121 acgaatcgct aaacgcaggc agcgggcgcg gcggcggtgt caattgagac aaaagtcggg
     6181 cggcgttggc ggcatccgat ctggtgttga tattcgcgtc cgtgttcatg accgcgtccg
     6241 cattctcgct cgcgctcgcg ccgtcaccgt ctgtctctac cgtggacggc ggcagcagaa
     6301 tcgggctttt tacgaaaacg agtgtcggaa taattaaatt catctgaatt ataaaggctg
     6361 agcgccgctg attcgtcttt tgttttttct ctgttcggct tcgatttgct tttgattggc
     6421 gcggcgaatc catttcacta gttcttcttt tattctgctc atcagcaaaa tatcttcgtc
     6481 caatctttcg gtctggtgct ggcgcaggta atcatagtta ctaccgattt tttgcagaca
     6541 ttcttttaag cggctgataa gttcgccgcg ctggtgttcg tcttcgattt tttggttgat
     6601 gtcttgttcg ataatcgcgt cgagcatttc gagcagaaat ctttcgatga ctttgtcctt
     6661 attgtggtcg ctgacaaaac aattgtttcg caattcgacg accagacgcg cttttgcttc
     6721 ctgaagtgaa tttttgagtt ccgagaaaag ctcgaagcct tcgtgcgcat tcaaaaaatt
     6781 ctcaatctcg ccggtctcgc ttgccgacag cgcgtcgggt attggcagcg tggcggtagt
     6841 gacggcgacc ggaagaacaa cgacggcgga ttcgtgttcg ccgctgaccg gcggctgaga
     6901 gtttccgttt tccggctgcg gcgattgcgg cgtcgttaac ggcggcgttt ggagcgacag
     6961 cagtgagctg tcttcgcatt tgtataaatt ttcgtcgcgc gtgagaacca gaacgatttt
     7021 cagcgggtaa gccagcgccg tcgtcaggtt gtagatttgt tccacgtttc ggtcttcaaa
     7081 atgctcttcg tccaaagaga gattaatcac cagcttgatg ctaaatgaat ctccatcatc
     7141 gcgcttcaag tatatcggcg ccgacttctg attccggttt agataaccgt aagccagaat
     7201 tttttccgtt cggctgtcgg tcaggataat ctctttagca taaagatgcc tccagtgatt
     7261 ttcccgcttt tttaaaaaat ccgagatttt gtcgtgcgtt tgcagaaaat ccgcgtcggt
     7321 cgtatgacaa aatctgcggt cgttatgagg caaataataa taattttgct cggcgctgtg
     7381 acgcccgtaa taatcggcga catacgtgcc ctgattacct ttggtaaaca gctcaaacca
     7441 gtattggctg atgccgaaga cggcttgcac catcaggctg ccggttttat aaactttcgt
     7501 gccgagttcg atttgttttc gcgccgtatc agagagccaa atcgtgcggc tgaatttgtt
     7561 gttttccttt aaagccgtct gttcggacga ggacgcggcg ggcgaaccga gaacttcgcc
     7621 gattactttt cttatttcct ttaagatatg ctcgaagcgc tcgtcgttcg attgattggg
     7681 atgctcgaaa agctgattta ttattccctt ccggttaaga cgatgcgtga tttcagcttt
     7741 ctgacggtcg gtcagcggcg cggacagtcc cattgcgccc gcatcctgaa gccgcgacat
     7801 ttc
//
LOCUS       DASWNE010001032         4464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_246177, whole genome shotgun sequence.
ACCESSION   DASWNE010001032 DASWNE010000000
VERSION     DASWNE010001032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246177"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="VGB68_19730"
     CDS             complement(<1..851)
                     /locus_tag="VGB68_19730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051538.1"
                     /translation="MNTLEEKKRFMRDYWLDSLTDNQTGQMETEWFGNDADAELLEIV
                     RSDLIEDYLTNSLNRTELDKFKNHFLINNLEEVAIAKSYLALGETPQKQIAEAGFFET
                     LAESWRSFVKIPQIAAAILLLGGLALFINFYNSEPVGIAQNEPPAFDGGDKNQIPQNH
                     VSSGETLPGEDNKSSDESPNNSQNSGEDSGKPTVVRNKTASEKAPEKSAPRAAQPQVL
                     FLTNFRGNVKTLKLADENRNFSLKLDMPGIDKAYKSYEVKIYDSNNNLVVRQKLNDNL
                     SLKKSGE"
     gene            complement(910..1539)
                     /locus_tag="VGB68_19735"
     CDS             complement(910..1539)
                     /locus_tag="VGB68_19735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051539.1"
                     /translation="MPYEAKKIYKKDWVLNAEAFEKLLCSLDGNPENASIIYEDIRNR
                     LIRQFRANQSQIAEEQADEVFNRIARKIYEEDFVLDKSNPYPYFHQTARYILLEQQRL
                     ARRKILGLEDLPFSEEPFYNPEEVLARFSERLRKEAGLNSLHECRESLSEKEINLLDR
                     YDAVKGKGRENLAKETGKTVNALKISINRIRKKLIDCAKRKLESIEAIE"
     gene            1737..2405
                     /locus_tag="VGB68_19740"
     CDS             1737..2405
                     /locus_tag="VGB68_19740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051540.1"
                     /translation="MKKAALGALGTSALVAGLGTTEKALARLFSRQSAAHVDEFLKRV
                     STGKTSAEIDSNFLKTFNSLMDIAARDERVQAGIDTIGDYVAQQVGETKGESVPPFGL
                     TQSVMDALAGGYLAASRGFENEAIPGESLAKYVANPEEVLSRVEPDFVTRLIPNLQEA
                     MRGDAELEKLVGTGNEHLQQVFRQIEQDPARAIICRINGKQVPCWLWGVVVVIVIVIV
                     IVLK"
     gene            3519..4157
                     /locus_tag="VGB68_19745"
     CDS             3519..4157
                     /locus_tag="VGB68_19745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051541.1"
                     /translation="MEKAGVHLAALEATLDKIEQSRVITPEDNESLDTNGLAMGDTLH
                     EAFQEASRSTLDAAESEGKNGNVEDLAYFESFEQNHTSRTEAIVVRTERIQKGIEDGT
                     IVLKQAPPTPVMIKAGFNRDTKPSLSPSLFEAADSSCLVAGSKSGSKILKPCVAPCLA
                     QRWVDCATCILRNVPAGIEQYNQFRNCWNNCSGFWKGFCRAKCLATFVYWIY"
BASE COUNT         1143 a         1046 c         1066 g         1209 t
ORIGIN      
        1 ttttcgccgc tttttttcaa agataaatta tcatttaatt tctgccggac gactaaatta
       61 ttattggaat cataaatttt tacttcgtag ctcttgtaag ctttatcgat tccgggcata
      121 tccagcttca gagagaaatt cctgttttcg tccgccagct tcagcgtttt tacatttccg
      181 cgaaagttcg tgagaaataa aacctgcggc tgcgcggcgc ggggcgcgga tttttccgga
      241 gctttttcgc tcgccgtttt atttctgaca accgtcggtt tgccggaatc ttcgccggaa
      301 ttttgactgt tattcgggct ttcatccgag ctcttattat cttcgcccgg aagcgtctcg
      361 ccgctcgaaa cgtgattctg cggaatttga tttttgtcgc cgccgtcaaa cgcgggcggc
      421 tcgttttgag caatgccgac cggctcggaa ttataaaaat ttataaacaa agccaagccg
      481 ccgagcaaaa gtatggcagc ggcgatttgc ggaattttta caaagctgcg ccagctttcc
      541 gccagcgtct cgaaaaagcc ggcttcggcg atttgttttt gcggcgtttc gcccagcgcc
      601 agataagatt tggcgattgc cacttcttcc agattgttta tcagaaagtg gtttttaaat
      661 ttgtcgagct cggttcgatt gaggctgttt gtcagataat cctctatcaa atcgcttctg
      721 acgatttcca gcagttccgc gtccgcgtca ttgccgaacc attcggtctc catctgcccg
      781 gtttgattat cggtcaaaga atcgagccaa taatcgcgca taaatcgttt tttctcttcc
      841 agagtgttca tcttaaccgt cccactaata aatgacaatt tcacaaaaaa gtttcataac
      901 cgccggaaat tattcgatgg cttcgatgct ttccagcttt cttttcgcac agtcgatgag
      961 cttttttctt atccggttga tggaaatttt caaggcgttg acggttttgc cggtctcttt
     1021 cgccagattc tcgcgccctt tgccttttac cgcatcatag cggtctaaaa ggttaatttc
     1081 tttttcgctc aaactttcgc ggcactcgtg cagagagttt aaaccggctt cctttctgag
     1141 tctctcgctg aagcgggcta aaacttcttc gggattgtaa aaaggctctt ccgaaaacgg
     1201 caaatcttcc aagcccagaa ttttgcggcg ggcgagtcgc tgctgttcga gcaaaatata
     1261 acgggctgtc tggtgaaaat agggataggg attgctttta tcaagcacga aatcttcttc
     1321 gtaaatctta cgcgcgatgc ggttaaaaac ttcgtcggct tgttcttcgg cgatttggga
     1381 ttgattggcg cgaaactggc gaatcagacg atttctaata tcttcataaa ttatcgaggc
     1441 gttttccgga tttccgtcca ggctgcataa aagcttttcg aaggcttcgg cattcaaaac
     1501 ccaatctttt ttgtatattt ttttagcttc atacggcatt cagtatttta aaaaacgttg
     1561 cggcaaaaaa aaatgtgaaa ccttttttca tttttgccaa ttatagctag aaagcctaca
     1621 aacgacaaac ttttctaaag tggctttcag aaacacgtcc tgactgagaa aattctgaaa
     1681 aacttaaaag gggagatttg acaatgaaaa agaataaaga gtctcggaga gattttttga
     1741 aaaaagccgc gctcggcgcg ctcgggacat cagctctggt tgccggcttg ggaacgacgg
     1801 aaaaagctct tgccagatta ttttcgcgcc aatcggcggc gcacgtcgat gagtttctga
     1861 aaagagtaag caccggaaag acttccgccg aaatagattc gaattttttg aaaactttta
     1921 attctctgat ggacatcgcc gcccgcgacg agcgagtgca ggcgggaatc gatactatcg
     1981 gagattacgt cgcgcagcag gtcggcgaga cgaagggcga gtcggttcct ccgtttggct
     2041 tgacccaatc cgtgatggac gctctggcgg gcggatatct ggcggcttcg aggggttttg
     2101 aaaatgaagc cattccggga gaaagcttag ccaagtatgt cgcaaatccc gaagaagtct
     2161 taagccgcgt cgagcctgat ttcgtaacgc gcttgattcc caatcttcag gaagcgatga
     2221 ggggcgatgc cgagcttgaa aagctggtcg gtaccggcaa cgagcatttg cagcaggtct
     2281 ttcgccaaat cgagcaggac ccggcgcgcg cgattatctg ccggataaac ggaaaacagg
     2341 ttccgtgctg gctctggggc gtcgttgtgg taatcgtcat cgtcatcgtc attgttttga
     2401 aataactttt gatttgcccg caacatattt tgagtttgcc cacaacatat tttgttgttt
     2461 tgaaaagatt agatgaaacg cgagccggtt tgcccacgcc gcccacatag cccacgtgcc
     2521 cacaaacccg catcgcccac ggtttcgtct gatgcaatcg aaaataaaag cctgacgctg
     2581 cggctgcatc gccgtcaggc ttttatcgag attcttaaag atatattgat attaatttaa
     2641 tcgtcgggag ccttggcggt cgttcgggaa tgttaagccc acatcgtttg aaagttttgc
     2701 cacaataaaa aaaccgaaaa cgatgaataa acattcccga acgacaaata aacagcttcg
     2761 tcgctgagga atttcttttt tgacttaaat ctcggtttat cactgaaaaa attaaaagca
     2821 ggcattgagg ggaaaagacg cgaaggattt ggggaaaact gattcctgaa atttctgctt
     2881 ttccagttta aactgaagat tatagtcttt acgggagcaa tcgggttttg aagtcgagct
     2941 gctcccgaaa aatttaaact cataatctaa tgaaaagcgg tatcggaaat catatttgag
     3001 tcgtttgacg atgaattatc cggcgcaaat cctttgttct atccaaaacg tgtcgtataa
     3061 ggctcggcaa acgattcaga tatgatttcc gataccgctt tccggtttcg ggcaaggctc
     3121 tgaatttgag gatgtccttg aaacttccgg caaccggcaa ataacgggta aacaatttat
     3181 ttacctaata acaaaaccaa aagcctgacg catcggctaa atgactatca ggctttcgga
     3241 caggtactct ttgtctgttt tcgcagatgg aatactgttt tttattttat ctccgaaacg
     3301 gcagaggctc aatatcactc aaaaaaactc caggagataa aaaaatgaaa accaaatact
     3361 tcttacctat tgcgattttc gcggtaattt gtttcacagc tttcggactc aatttcaatt
     3421 cgaatactct acaggctgcg ccccggattt cggagaaaga aaacgcttcg ctgactgcgt
     3481 cgcccgtcaa gcctccgtct gattcattaa gagcaaaaat ggaaaaggct ggagtgcatc
     3541 ttgcggcttt ggaagcgacg ctggataaaa tcgagcaaag tcgcgtcatc acgccggaag
     3601 acaacgaaag cctggacacg aacggattag cgatgggcga tacgctgcac gaggcttttc
     3661 aggaagcttc gcgctcgacg ctggatgccg ccgaatccga agggaaaaac ggcaacgtcg
     3721 aagacctggc gtatttcgaa tcgtttgaac aaaatcacac gagccggacc gaagccatcg
     3781 tcgtcagaac cgagcggatt caaaagggca tcgaggacgg aacgattgta ttaaaacaag
     3841 ctcctccgac gccggtgatg attaaagccg gtttcaaccg ggacaccaaa ccgagcctta
     3901 gcccgagcct gttcgaggcg gcggattctt cgtgcctcgt cgccgggtca aaatccgggt
     3961 cgaaaattct gaaaccgtgc gttgctccgt gtctggcaca gagatgggtg gactgcgcca
     4021 cctgcattct cagaaacgtt cccgccggaa tcgagcaata caaccagttc cgcaactgct
     4081 ggaataattg cagcggcttc tggaaaggtt tctgccgggc taaatgctta gcaaccttcg
     4141 tttactggat ttattaatgc ccgcgggcag aagttttccg gcggtagtcc gccagccgat
     4201 tgaagttcgc gaaccggctt gaagcacggc ttcgaatata tgtatttcaa tcggctggac
     4261 gttccgcgcg aaaactccgc cgggctgtcg cgaagacagc gttccggttt tgcgccgttt
     4321 agaagaggaa acagcgcaaa aagaatcgtc cgtgatgatt cgtcgacctt tattccgtta
     4381 tgcggaggtt tcggcggttt ccactctttc gacaattcat ttccggaaca ctgcttttcc
     4441 tcaacccgcg cgtcgtcgcg aaaa
//
LOCUS       DASWNE010001033         1715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_345515, whole genome shotgun sequence.
ACCESSION   DASWNE010001033 DASWNE010000000
VERSION     DASWNE010001033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345515"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1715
                     /locus_tag="VGB68_19750"
     CDS             <1..>1715
                     /locus_tag="VGB68_19750"
                     /inference="COORDINATES: protein motif:HMM:NF027787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY0051542.1"
                     /translation="GRLVEKEELMKMVWADSFVEAGNLSFTMRLLRKALGDDTQNPRF
                     IETVPRRGYRFIAEVRVAAKENASHNDLPAPLARPVEEKPRPPGSGFDKALLPVFALL
                     FAAVILIGFWFFKSKNFSSVAPVLDAPFSSENLSTNGKVPLAVLSPDGKNVIYTNGMS
                     GDKQSVWLRQLETGSNVEIIPPSDEQYFGLALSPDEKTLYFARRSRSFREPTDIYRVS
                     IFGGVPEKIVTGTELGWMGISPDGKQISYVRCPQQSDEFCSLNVADSADGKNERKILS
                     RPYPFRIGANRFAPDGKSIAFAAGTSVGSSNEFELWEVELDGGERNLTDQKFFDIKNL
                     AWLPDKSGLLITASRFPNKNFRIWQVSAGSGDASPLTKDSESYAVLNMNKEASAIVST
                     RFKEDFRIKLIPLENPSQTTTLTNASAASFAPDGRIYFSSIISGNDEIWSINADGSGQ
                     RQLTNNAADEFAPFISPDNKTVFFVSNKSGSAQLWRMNTDGSNQTQITETDGGAPVFV
                     SPDGEWVYYRHILTSVLWRISTKSGERQLVLNKEKPRFAFSPDGKQVAFSEEQDDERI
                     LTIVS"
BASE COUNT          419 a          454 c          446 g          396 t
ORIGIN      
        1 acggtcggct ggtcgaaaaa gaagagttga tgaaaatggt ttgggcggat agttttgtcg
       61 aagcgggtaa tctgtctttt acgatgcgcc tgctgcgcaa agccctcggc gacgacacgc
      121 agaacccgcg ttttatcgaa acggttccgc gacgcggtta cagatttatc gccgaggtca
      181 gggtcgccgc aaaagaaaac gcctcgcata acgatttgcc cgctcctctt gcccgacctg
      241 tcgaagaaaa gccgcgaccg ccgggctcgg ggttcgataa agcgctgctt ccggtcttcg
      301 ctcttttatt tgccgccgta attttaatag gcttctggtt tttcaaaagc aaaaacttct
      361 cctcggtcgc gcctgtttta gacgcgcctt tttcttcgga aaatctttcg accaacggaa
      421 aagtcccgct tgccgtcctt tcaccggacg gcaaaaatgt gatttataca aacggtatga
      481 gcggcgataa gcaaagcgtc tggctgaggc agctcgaaac cggcagcaac gtcgagataa
      541 ttccgccgtc cgacgaacaa tacttcgggc tcgccctctc gcctgacgaa aaaactcttt
      601 atttcgctcg tcgctcgcgt tctttccgtg agcctacgga tatctaccgc gtttcgattt
      661 tcggcggcgt accggaaaaa atcgtgaccg ggacggagct cggctggatg ggcatttcgc
      721 cagacggcaa gcaaatctct tacgtgcgat gccctcaaca gagcgacgaa ttctgttcgc
      781 tgaacgttgc cgattcagcc gacggcaaaa atgaaaggaa aatcctgtcg cgcccgtacc
      841 cgtttcgcat cggcgccaat cgctttgcgc ccgacggaaa atcaatcgcc ttcgccgcgg
      901 gaacatcagt aggttcttcg aatgagttcg agctctggga agtcgaattg gacggcggcg
      961 agcgcaattt aaccgaccaa aaatttttcg acatcaaaaa cttagcctgg cttccggaca
     1021 aaagcggtct gctgattacg gcttcgagat ttcccaacaa aaactttcgc atctggcagg
     1081 tttccgccgg aagcggcgac gcttcgccgc tgaccaaaga ttccgaatcc tacgcggtgt
     1141 taaatatgaa taaggaggca agcgcgatag tttcgacgcg gtttaaggaa gactttcgga
     1201 ttaagctgat tccgctggaa aacccttcgc agacgaccac gttaaccaac gcgtcggcgg
     1261 cgtcattcgc cccggacggc aggatttatt tttcttcgat tatttcgggc aacgatgaaa
     1321 tctggagtat aaacgccgac ggcagcgggc agcggcagtt aaccaataat gcggcggacg
     1381 aattcgcccc gtttatttcg cccgataata aaacggtatt tttcgtgtca aacaagtcag
     1441 gctccgcgca gttgtggcgg atgaatacgg acggcagcaa tcaaacacaa attaccgaaa
     1501 cggacggcgg cgcgcccgtt tttgtttcgc ccgacgggga atgggtttat taccgtcaca
     1561 tcctgaccag tgtgctttgg cgcatctcga cgaaaagcgg cgaaaggcag cttgttttga
     1621 ataaagaaaa accacgcttt gccttttcgc cggacggcaa acaggtcgct ttttccgaag
     1681 aacaagacga cgaaagaatt ttaactatcg tctca
//
LOCUS       DASWNE010001034         1876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_69890, whole genome shotgun sequence.
ACCESSION   DASWNE010001034 DASWNE010000000
VERSION     DASWNE010001034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69890"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VGB68_19755"
     CDS             <1..597
                     /locus_tag="VGB68_19755"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEY0051543.1"
                     /translation="CIKCGARNSDEAIYCQKCGGLLEAEEETRVARQPQKEAVRDEFE
                     LEEKEIFSVRPTLMFIYAGYAAAVAGAFLLVVMLYYFGQLIGIDIPWWLSILTGLLLL
                     LIPAFYHFKRSIVRYTLTDSKIEIDEGFIFQKTHNIPLRSIQHVSVSSTITQRMLGFG
                     NLIIDNASEGDEKIVLKNINSPKQLADIMLRQMRQINK"
     gene            690..1715
                     /gene="rfbB"
                     /locus_tag="VGB68_19760"
     CDS             690..1715
                     /gene="rfbB"
                     /locus_tag="VGB68_19760"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007846915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEY0051544.1"
                     /translation="MKTIFVTGAAGFIGSAFVRLILKETSDFEIINFDALTYAGNPEN
                     LISLEDEPRHRFVQGDITHREAVLNSLPENCEAIFNFAAESHVDRSIHSADEFLKTNI
                     IGTQILLDAAREKNTRRFVQISTDEVMGSLPDESDLYFTEDSPLQPNSPYAASKASAE
                     MVVRAARETFGIDAVITRCGNNYGERQFPEKLIPLMISNALLDEPLPVYGDGRNVRDW
                     IYVEDHCRAVWMIYEKGKAGEAYNVGARNERENIEVVTAILDALNKPHSLIKFVTDRL
                     GHDRRYAINPEKIEREIGWKPQTSWQEGLQKTIDWYLDNQDWVSRVRDGKYRDYYQSM
                     YGASLKA"
BASE COUNT          486 a          486 c          473 g          431 t
ORIGIN      
        1 tgcataaaat gcggcgcgag aaattccgac gaagcgattt actgccagaa atgcggcggc
       61 ttgctggaag ccgaagaaga aacgcgggtc gcgcgccaac cgcagaaaga agcggtcagg
      121 gacgagttcg aacttgaaga aaaagaaatc ttttccgttc gcccgacgct gatgtttatt
      181 tacgccggct atgccgcggc ggtggcgggc gcgtttttgc tggtcgtgat gctctattat
      241 ttcgggcagc ttatcggcat cgacattcct tggtggcttt cgattctcac cggtttgctt
      301 ttgcttctga ttccggcttt ttatcatttt aaacgcagca tcgtgcgcta cacgctgacc
      361 gattcgaaga ttgaaatcga cgaaggcttc atttttcaga agacgcacaa cattccgctt
      421 cgctcgatac agcacgtttc ggtttcttcg acgattacgc agcgaatgct cggtttcgga
      481 aatttaatta tcgacaacgc cagcgaaggc gacgaaaaaa tcgttttaaa aaacatcaac
      541 tcgccgaaac aactcgccga cattatgctt cggcagatgc ggcagattaa caagtgaatt
      601 acgaattacg aattacgaat tacgaattaa aagaaacttt ccatcattcg taattcgtaa
      661 ttcgcaattc gtaattttac aattgattta tgaaaactat ctttgttacg ggcgccgcgg
      721 gctttatcgg ctcggctttc gttcgcctga ttctgaaaga aacttccgat tttgaaatta
      781 tcaatttcga cgcgctgacc tacgccggaa atcccgaaaa tttaatcagt ctggaagacg
      841 agccgcgcca ccgcttcgtg cagggcgata taacgcaccg cgaagccgtg ctgaattcgc
      901 tgccggaaaa ttgcgaagcg attttcaatt tcgccgccga atcgcacgtc gaccgcagca
      961 tccattcggc tgacgaattt ctgaaaacga acatcatcgg aacgcagatt ttgctcgacg
     1021 cggcgcgcga gaaaaacacg cgtcgtttcg tacagatttc gaccgacgaa gtgatgggca
     1081 gcctgcccga cgagagcgat ttgtatttta cggaagattc gcccctgcaa ccgaattcgc
     1141 cttatgcggc gtcaaaagcc tccgccgaaa tggtcgtccg cgccgcgcgc gaaactttcg
     1201 gcatcgacgc cgtgataacc cgttgcggca acaattacgg cgaacgccag tttcccgaaa
     1261 agctgattcc gctgatgatt tccaacgcgc ttctggacga gcctctgccc gtctacggcg
     1321 acgggcgaaa cgtgcgcgac tggatttacg tcgaagacca ttgccgcgcc gtctggatga
     1381 tttacgaaaa aggcaaagcg ggcgaagcct acaacgtcgg cgcgcgaaac gaacgggaaa
     1441 acatcgaagt cgtcacggcg attctcgacg ctttaaacaa gccgcattcg ctcatcaaat
     1501 tcgtcaccga ccgcctcgga cacgaccgcc gttacgcgat caatcccgaa aaaatcgagc
     1561 gggaaatcgg ctggaagccg caaacctcgt ggcaggaagg cttgcagaaa actatcgact
     1621 ggtatctgga caatcaggat tgggtttcgc gcgtccgcga cggcaaatac cgcgattatt
     1681 accagagtat gtacggcgct agcctgaaag cgtaaaaata tgaaaatcct aatcaccggc
     1741 gcaaacggaa tggttgccgg agccgcgatt aattattacc gctcgctcgt cgacgacgtt
     1801 ttgtctttca cgcgcgagga gcttgatatt gccgacagga aacaggtttt tgcgattttt
     1861 gaagaaacaa aacccg
//
LOCUS       DASWNE010001035         3511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_34670, whole genome shotgun sequence.
ACCESSION   DASWNE010001035 DASWNE010000000
VERSION     DASWNE010001035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34670"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..518)
                     /locus_tag="VGB68_19765"
     CDS             complement(93..518)
                     /locus_tag="VGB68_19765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051545.1"
                     /translation="MDIRTNLSPEAAKKIFGNQEKAIAFLLELQKVKQIDLPIKIIKS
                     GDNKGKESVNLKTADEIWSKIAEHWDFEASQKIVRKLFKTTDKYKSGRNSYATMNILF
                     EEWNRLNFGEIKWSVSQGEFDGFVQAVNKKKYQGLSKTD"
     gene            complement(528..1331)
                     /locus_tag="VGB68_19770"
     CDS             complement(528..1331)
                     /locus_tag="VGB68_19770"
                     /inference="COORDINATES: protein motif:HMM:NF014177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEY0051546.1"
                     /translation="MKFAIMKYNDLDIENWKDCNINTDSLWLISERDKSGKHENIYHG
                     NFVPQIPNQLVRRYTKKSETVFEPFMGSGTTLFECEKLNRKYIGFDINSKIVDYVQQK
                     VSAESYFFIKECNTLDSQKADDNFETAFDKLSVKSVQFVLMHPPYLDIVKFTDNPNDL
                     SQIGNLKDFIGKFKTVCENSLKYLDKNRYFAIVGGDVYKNGEVLPLAFYCMDMIKRSF
                     SVKLKGIIIKNIEGNRGKIGQNNIWKYRALNSDYFIFKHEYIFVFKKDF"
     gene            complement(1341..1757)
                     /locus_tag="VGB68_19775"
     CDS             complement(1341..1757)
                     /locus_tag="VGB68_19775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051547.1"
                     /translation="MQFEDLGKNLPALFSALLVSILFIQSGLDKVFDWKGNLEWLTGH
                     FSKTFLRGTVPPMLAAITLMELATGVLSAAGIIYFLAAASTVLIFYASVLGALSIVAL
                     FFGQRVAKDYPGAAVLVPYFILLLILMHLTNPFLKA"
     gene            complement(1932..3257)
                     /locus_tag="VGB68_19780"
     CDS             complement(1932..3257)
                     /locus_tag="VGB68_19780"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HEY0051548.1"
                     /translation="MKPQLKNFIEQIGKDAIAIIPAAHETTRSYDTEHKFRQDSDFLY
                     LTGFPEPDAIAVINPSSRKNPFTLFVRPRDPEMETWYGRREGTDGAVKNYKADKAFPI
                     EKFEQELPKLLDGREKLYYRFGVDNKLDQVILQYLSGQRFRRLKTAYPPHTIIDPTVL
                     IHEMRLHKTPEEVELMQTSATIAAEAHILAMQSVKPGMNEYQVESLMESYMRHHGASG
                     VAYNSIIGGGINACILHYVENNCPLQDGDLILIDAGAEYKGYASDITRTFPVNGRFTK
                     PQREVYDVVLDVQLQCIEATQKGNTIKQRQELSIELLTEGMKKLGLLKGKTRDLIKKK
                     KFMKYYMHGVGHYLGMDVHDAGRYFTEQQAKNSRPFAPGMVLTVEPGLYIPPDDKDAP
                     GKYRGIGIRIEDDVLVTEDGNVNLTSKVPKDADAIEELMNTAKAASAIK"
BASE COUNT          881 a          779 c          774 g         1077 t
ORIGIN      
        1 ttcattcttt tcaaatatta gagtttcgat aaagtcgtgt ctaacagtat taatttcttt
       61 tattcttctg tatcttactg ccgcgagttt tactagtctg tctttgatag tccttgatat
      121 ttttttttat ttactgcttg aacaaagccg tcaaattctc cctgagaaac tgaccacttt
      181 atttctccga aatttaatct attccattct tcaaacaaaa tattcattgt cgcgtaagaa
      241 tttctacccg atttgtattt atcggtggtt ttgaaaagtt tccttactat tttttgactt
      301 gcttcaaaat cccaatgctc agcaattttt gaccatattt catctgccgt ttttaaattg
      361 accgattctt tccctttatt atctccgctt ttgattattt taatcggcaa gtcgatttgt
      421 tttacttttt gtagctccaa aagaaatgca attgcttttt cctgattgcc gaaaatcttt
      481 ttggcagctt cgggtgaaag atttgtcctt atatccataa aagattttta aaagtccttt
      541 ttgaaaacaa aaatatattc gtgtttaaag ataaaatagt cgctgttcaa agcgcgatat
      601 ttccagatgt tattctgtcc gatttttccg cgattgcctt cgatgttttt gatgatgatg
      661 ccttttagtt ttactgaaaa acttcgcttt atcatatcca tacaataaaa cgccaaaggc
      721 aaaacttcgc cgtttttata aacatcgccg ccgacgattg caaaatatct gtttttatcg
      781 agatatttca gcgaattttc gcaaaccgtt ttgaatttgc cgataaaatc tttgagattg
      841 ccgatttgcg atagatcgtt tggattatcg gtaaatttta cgatgtccag ataaggcgga
      901 tgcatcaaaa caaactgtac gcttttcaca gacaatttat caaaagcagt ttcaaaattg
      961 tcatcagctt tttgagaatc gagcgtatta cattccttga tgaaaaaata gctttcagca
     1021 gagactttct gctgaacata atcaacaatc ttggaattta tatcaaagcc gatatatttt
     1081 ctgtttaatt tttcgcattc aaaaagcgtc gttccgcttc ccataaaagg ctcaaaaaca
     1141 gtttcgctct ttttcgtgta gcgtctgact aactgatttg ggatttgcgg aacaaaattt
     1201 ccgtgataaa tattttcgtg tttaccgctt ttgtcacgct cgcttataag ccacaatgaa
     1261 tcagtattga tattgcaatc cttccaattt tcaatatcca aatcattgta tttcataatc
     1321 gcaaatttca ctgcctttcg ttaagccttc aaaaacggat tggtcagatg catcaaaata
     1381 agcagcagga taaaatacgg aaccagaacc gccgcgcccg ggtaatcttt ggcgacgcgc
     1441 tgtccgaaga aaagggcgac gattgagaga gcgccgagga cggatgcgta gaatatcaga
     1501 acggtcgagg cggcggcgag aaaatagatg attccggcgg cgctcaaaac tccggtggct
     1561 agttccatta aagtgatggc ggcgagcatc ggcggaaccg ttccgcgcag gaaggttttg
     1621 gaaaaatgtc cggtcagcca ttcgagattg cctttccagt cgaagacctt gtcgagtccg
     1681 gattggatga aaagaatgct gaccagcagg gcgctgaaaa gcgcgggaag atttttgccc
     1741 aagtcttcaa attgcatttt gtttttgtaa aagaagccag ttagctgaaa ctgccgtgaa
     1801 ctttttggag atttgcccgc gaaatgcgcg aaagaggcga aaaagcgtga aagctgtcgg
     1861 gattttcgct ttttcgtttg attcgcgcat ttcgcgggca aagttttttc ataatcggta
     1921 tcttcgatta attattttat cgcgctcgcc gcctttgcgg tgttcatcaa ttcttcaatc
     1981 gcatcggcgt ctttcggcac tttcgaggtc aggttgacgt tgccgtcctc ggtgaccaaa
     2041 acgtcgtctt cgatgcgaat gccgatgccg cgatatttgc cgggcgcgtc cttatcgtcg
     2101 ggcggaatgt aaagcccggg ctcgacggtc agaaccattc cgggcgcgaa cgggcgcgag
     2161 tttttcgcct gctgctcggt gaaatagcgt ccggcgtcgt gcacgtccat gccgagataa
     2221 tgcccgacgc cgtgcatata atatttcata aactttttct ttttgatgag gtctctggtt
     2281 ttgcctttca gcagaccgag ctttttcatt ccttcggtca gaagctcaat cgaaagctcc
     2341 tggcgctgtt tgatggtgtt gcctttttgg gtcgcttcga tgcattgcag ctgcacgtcg
     2401 agaacgacgt cgtaaacttc gcgctgcggt ttcgtgaaac gcccgttgac cggaaacgta
     2461 cgcgtgatgt cggaggcgta gcctttgtat tcggcgcccg cgtcaatcaa aatcaaatcg
     2521 ccgtcctgaa gcgggcagtt gttttcgacg taatgcagaa tgcaggcgtt aatgccgccg
     2581 ccgatgattg aattataggc gacgccgctc gcgccgtgat gccgcatata agattccatc
     2641 aaggactcga cctgatattc gttcattccg ggtttcacgc tctgcatcgc cagaatgtgc
     2701 gcttcggcgg cgatggtcgc gctcgtctgc atcagctcga cttcttccgg cgttttatga
     2761 agccgcattt cgtgaatcaa aacggtcggg tcgataatcg tgtgcggcgg ataagcggtt
     2821 ttcagacggc gaaagcgctg accggaaaga tactgcaaaa tcacctggtc gagcttgtta
     2881 tcgacgccga agcggtaata aagcttttcg cgcccgtcca gcagcttcgg caattcctgc
     2941 tcgaattttt caatcggaaa ggctttatcg gctttataat ttttcaccgc gccgtccgtg
     3001 ccttcgcgcc tgccgtacca cgtctccatt tccggatcgc gcgggcgcac aaacaacgta
     3061 aagggatttt tccggctcga cgggttaatg acggcaatcg cgtcgggttc gggaaatccg
     3121 gtcagatata aaaaatcgga atcctgccgg aatttatgct ccgtgtcgta gctgcgcgtc
     3181 gtttcgtgcg ccgccggaat aatggcaatc gcgtctttgc cgatttgttc gataaaattt
     3241 tttaattgcg gtttcatctg tttctcctct tttccgaaat tgtaacgcaa agttttaaaa
     3301 agcggaaaat tcgacgcgga aatacaaaca aagaattaac cgtcgataaa gcggctcgga
     3361 cggcgattaa aagaaaagac gatttaagat ttcatattta tccgcttcat ccgcggttaa
     3421 ttttctgcgc cgattgtaat gaattatccc gcaaaaaaaa taaaatttac cgttccgttt
     3481 ttaaaaagaa ttgctataat cgacggcact t
//
LOCUS       DASWNE010001036         6281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_630531, whole genome shotgun sequence.
ACCESSION   DASWNE010001036 DASWNE010000000
VERSION     DASWNE010001036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6281
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630531"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VGB68_19785"
     CDS             <1..835
                     /locus_tag="VGB68_19785"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY0051549.1"
                     /translation="VSGIGTGGTISGTGRYLKEKNPDIRIVGADPYGSIFKNYKDSGR
                     VPEATPYLVEGIGQSLPVGNADMKAIDEIINITDRESFEFARQLSRVEGIFCGGSTGT
                     NFAAALKVAKNLDENGLVVFIVCDTGEHYLTKFHSDEWMKEKLLLEPQRITAGLIAGT
                     KNSHSPGELLFVAPGETVSEALAKMSENGVTQLPVLEENRSVGSLRESRVLAKLLADG
                     NLLSAQVSEVMDESFPVVDIDTSIQEIKPKLQESPAVLIEDFKRITGIITRSDVLDLP
                     R"
     gene            1016..2551
                     /locus_tag="VGB68_19790"
     CDS             1016..2551
                     /locus_tag="VGB68_19790"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0051550.1"
                     /translation="MKATLRINGENDFVIEDDLVTIGRASDNKLALDDDSNVSRYHAE
                     IENRGGEFWLVELGSSNGTTVNDTPLETEILLRDGDVILLGGTSEIEFCIQPEKPEKP
                     EKSSAVAPPSINANLRDANVNASAAPIAPEAGQAAKMPVMLIVMGLVCGLAVICVVGA
                     ALYSYNSAPAQCDAKVVITSPENGDTINKETEITVEAENTDCVSRAVFLLDGTEIASA
                     DSAPYTATLDPKQFPALSDGGNHSLKVVLEDASGNLIAPSEIFLALETAEIATPTPTP
                     VIEETPVPTVTQPTGKQASLIDIQEMSRRLVKQFSGNFAYKFDQQFLQEVQKKTPEYA
                     AAEGYFARAETYRDLINLEFHKENGLDAPLGFLLAMSRSKFNPQKQGDEEGLWKLSDE
                     FVSTNGYKTICQAETLGDATQNCAAKTSSIYAKALVLKIFNGDVVYAAAAFGMSEQEA
                     STWASSLPPAREDFWKVINSAKQREQVARFFAAGIVAENPQKFGMKKDKPISELYRNL
                     MGN"
     gene            2653..4131
                     /locus_tag="VGB68_19795"
     CDS             2653..4131
                     /locus_tag="VGB68_19795"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0051551.1"
                     /translation="MLEVFLTYPTPEGSREIALDSDRISMGRGSDADLRFDDDGLSRL
                     HATIHRDGEKVWILDENSTNGTFVNGEKVPPAGTPLYDGDRVKIGHYTDLRIRFAQPQ
                     NEKTTPAENKSLAQSAVAASSDASGSFGILPVALTAFAIFVIAISAAFIAIQALVGDG
                     GDPQIAQNTRNANKQIIDDNGDPLEEDTPKPSPKPTREANTNQTVGANSNLTTSPEDA
                     NKNIISSLPRKKFADMSEPEKDEYVKVKAEKIARIIGNQTSEPIPPEAVRSIRGHLKG
                     YIDRVRAARVNNCGQGAFTRSDMTTVMERASKNAPFILRAFYAEGLDPQIGLYVAMIE
                     SEHCPCLQSPTGALGMFQFVRGTGREYNLQVKNDASPSNPDQRCEPEPAARAAAQYLK
                     YLTGRFGTGPMSFPLAIASYNSGQGGLSQNLEKALKAAAGQDRSFWTLVANQVAFEGG
                     IGEQFRRENVKYVPKFFAAAIVGENPADFGIQMQPLSTYTKE"
     gene            4211..5506
                     /locus_tag="VGB68_19800"
     CDS             4211..5506
                     /locus_tag="VGB68_19800"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0051552.1"
                     /translation="MTELWLNYTDETGETRRILVEGERFVIGRHSENDLSISDGSLSR
                     RHAQIERFGDIFVVSDLNSSNGTTLNEAPLEAPVALKKGDKFNLGGGFEFEVELISGD
                     ENADDAAGAAAQKDSEEQAGAASGAAEKASAASFSAAPGGNSVSMNFFILAPLLGVFV
                     LLFVGGGLFLLSGNGKEVSGNQGNFIRSETPERIPRAANTGDETPTPSVKPSVGASQT
                     PSNSTVNSAPTPQNSGELDKIERSALLFTRQIAINDNNYVFARGQLNEINNRLKNFKG
                     SDALRDNLKAVKQNAAQFEQLAKSKGLKPQFLATAALARIGNQRGNPLQTAQSMLNVL
                     GELKISLGNELADENLLIIAAFEQGERGEFRAMRNTVEVLSKQPGVDSDRARTIWFLR
                     EKGKLSDAQYEFALRFLAIGAISQNPADFNVETDAVVFN"
     gene            5619..>6281
                     /gene="tssK"
                     /locus_tag="VGB68_19805"
     CDS             5619..>6281
                     /gene="tssK"
                     /locus_tag="VGB68_19805"
                     /inference="COORDINATES: protein motif:HMM:TIGR03353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HEY0051553.1"
                     /translation="MSKYRKVVWNEGMLLTPQHFQQWDNYHEELLNSRVRSVQPYEYG
                     VLDLQVNREAIANGSFQLTGCRAVLPDGLMINVPDTNAVPDLRPVGEHFNREKETLGV
                     HLAIPALKFGSANFQGNGVKPNANLRFLQEGSLIKDETSGTNEQPLAFARPNLRIIFD
                     DELRDGFTSIKIAELQRTTTGQLQISENYIPPVLKVSASDWLVNMLRQLVEILITKSS
                     SLG"
BASE COUNT         1587 a         1646 c         1730 g         1318 t
ORIGIN      
        1 cgtttcgggc atcggcacgg gcggcaccat cagcggaacg gggcgctacc tgaaagaaaa
       61 aaatccggac atccgtatag tcggcgccga cccttacggc tcgattttca aaaattacaa
      121 ggattcggga cgcgtgcccg aagcgacgcc ttatctggtc gaaggcatcg gacaatctct
      181 gccggtcggc aacgccgata tgaaagcgat tgacgaaatc attaatatca ccgaccgcga
      241 atcgttcgag tttgcgcggc agctctcgcg cgtcgaagga attttctgcg gcggctcgac
      301 cggcacgaat ttcgccgccg cgctgaaagt ggcgaagaac ctggacgaaa acggtctggt
      361 ggttttcatc gtctgcgaca cgggcgagca ttatttaacc aaatttcact cggacgaatg
      421 gatgaaggaa aagcttctgc tcgaaccgca gcgcatcacg gcgggcttga ttgccggaac
      481 gaaaaattcg cactcgccgg gcgagcttct tttcgtcgcg cccggcgaaa ccgtctcgga
      541 agcgctcgcc aaaatgagcg aaaacggcgt cacgcagctt cccgttctgg aagaaaaccg
      601 ctcggtcggc agcctgcgcg aatcgagagt tctggcgaaa ctgctcgccg acggcaatct
      661 gctgagcgcg caggtcagcg aagtgatgga cgagagcttt ccggtcgtcg atatcgacac
      721 cagcattcag gaaatcaagc cgaagctgca ggaatcgccc gccgttctga ttgaagattt
      781 caaacgcatc acgggaatca tcacgcgctc ggacgttctg gatttgccga gataagaaat
      841 ctcaactcgg acaaacactg aagagaattt acagggataa caggatagac agggattaag
      901 aaacagatat ttatatcccg ttcatcctgc tatccctgta aatttctttc tttcacgcgc
      961 tttcgcggct catctctttt tctcaatttt catttatggt ttaatctaag cgaagatgaa
     1021 agcgacgctc agaatcaacg gcgaaaatga ttttgttatc gaggacgacc ttgtcacaat
     1081 cggtcgcgct tcggacaata aactggcgct tgacgatgat tcgaacgtct cgcgctatca
     1141 cgccgaaatc gaaaatcgcg gcggtgagtt ctggctcgtc gagctcggca gcagcaacgg
     1201 cacgaccgtt aacgatacgc cgctggaaac ggaaatttta ttgcgggacg gcgacgtaat
     1261 tctgctcggc ggaacaagcg aaatcgagtt ttgcattcag ccggaaaaac cggaaaaacc
     1321 ggaaaaatct tcagccgtcg cgccgccgtc gattaatgca aatttacgag acgcgaacgt
     1381 aaacgcttcc gccgcgccaa tcgcgccgga agcggggcaa gccgcgaaaa tgcctgtgat
     1441 gttaatcgtt atgggcttgg tttgcggact ggcggtcatt tgcgtcgtcg gagcggcgct
     1501 ttattcttac aattccgcgc cggcgcagtg cgatgcgaaa gtcgtgatta ccagtcccga
     1561 aaacggcgac acaatcaata aggaaaccga aattacggtc gaagcggaaa ataccgactg
     1621 cgtgagccgc gccgtttttc tgctcgacgg cacggaaatc gcttccgccg attccgcgcc
     1681 ttacacggcg acgctcgacc cgaaacaatt tccggcgctc tccgacggcg gaaatcattc
     1741 gctgaaagtc gtgctggaag acgcttccgg caatttgatt gcgccgagcg agatttttct
     1801 ggcgcttgaa accgcggaaa tcgccacgcc gacaccgacg ccggttatcg aagaaactcc
     1861 cgttccgacc gtcacccaac cgaccggaaa acaggcttcg ctgatcgaca ttcaggaaat
     1921 gtcgcggcga ctggtcaaac aattctccgg caatttcgca tacaaattcg accagcagtt
     1981 tttgcaggaa gtacagaaaa aaacgccgga atacgcggca gcggaaggct atttcgcccg
     2041 cgccgaaaca tatcgcgatt tgataaatct cgaatttcat aaggaaaacg gcttggacgc
     2101 gccgctcggt tttctgctgg cgatgagccg cagtaaattc aacccgcaaa aacagggcga
     2161 cgaggaaggc ttgtggaaac tatctgacga attcgtctcg acaaacggct acaaaacgat
     2221 ttgccaggcg gaaacgctcg gcgacgcgac gcaaaattgc gccgccaaaa cgtcttcaat
     2281 ttacgcgaaa gcgctggttt tgaaaatttt taacggcgac gtggtttacg ccgccgccgc
     2341 tttcggaatg agcgagcagg aagccagcac gtgggcgtcg agtttgccgc cggcgcgcga
     2401 agatttttgg aaagttatca attccgcgaa acagcgcgag caggtcgccc ggtttttcgc
     2461 cgccggaatc gtggcggaaa atccccaaaa attcgggatg aaaaaagata aaccgatttc
     2521 ggaattgtat cgtaatttga tggggaatta agaaacggtc ttcggtcttt ggtcttcggt
     2581 cttcgatatt ttttatttaa cagtgagcat aattctcttg cgaagaccga aagccgaaga
     2641 ccgttttaaa atatgcttga agtttttctg acatatccga cgccggaagg ctcgcgggaa
     2701 attgcgctcg acagcgaccg aatctcgatg ggacgcggca gcgatgccga tttgcgtttt
     2761 gacgacgacg gattgtcgcg cctgcacgcg accattcatc gcgacggcga aaaggtctgg
     2821 attttggatg aaaactcgac caacggcaca tttgtcaacg gcgaaaaagt tccgcccgcc
     2881 ggaacgccgc tttatgacgg cgaccgcgta aaaatcggtc attacacgga tttgcgaatt
     2941 cgctttgcgc agccgcagaa cgaaaagaca acgccggcgg aaaataaatc tttggcgcaa
     3001 tccgccgttg ccgcatcgtc ggatgcttcc ggctcgttcg gaattttgcc cgtcgcgctg
     3061 acggcttttg cgattttcgt gattgcgatt tcggcggcat ttatcgccat tcaggcgctc
     3121 gtcggcgacg gcggcgaccc gcagattgcg caaaacactc gcaacgcaaa caaacaaatt
     3181 atcgacgaca acggcgatcc gctcgaagaa gacacgccga aaccgtcgcc gaagccgacc
     3241 cgtgaagcca acacaaatca aacggtcgga gcgaattcaa acctgacgac ttcgcccgaa
     3301 gacgcgaata aaaatattat aagttcgctg ccgcgcaaaa aattcgccga tatgagcgag
     3361 ccggaaaaag acgaatacgt caaagtcaaa gcggaaaaaa tcgcgcgcat tatcggcaat
     3421 cagacgagcg agccgattcc gccggaagcc gtgcgcagca ttcgcgggca tctgaaaggc
     3481 tatatcgacc gcgtgcgcgc cgcgcgcgta aataattgcg ggcagggcgc ttttacgcgc
     3541 agcgatatga cgaccgtgat ggaacgcgcc agtaaaaacg cgccgtttat cctgcgcgct
     3601 ttttacgccg aagggctcga cccgcaaatc ggcctgtatg tggcgatgat tgaatccgag
     3661 cattgcccgt gtctgcaaag cccgaccggc gcgctcggaa tgtttcaatt cgtccgcggg
     3721 acgggcagag aatacaatct gcaagtgaaa aacgacgcgt cgccgtcaaa tcccgaccag
     3781 cgctgcgagc cggaaccggc ggcgcgcgcg gcggcgcaat atttaaaata tctgacgggc
     3841 agattcggca ccggaccgat gagctttccg ctggcgattg ccagctacaa cagcggtcag
     3901 ggcggcttga gccagaatct ggaaaaggct ttaaaagcgg cggccggaca ggatagaagc
     3961 ttctggacgc tggtggcaaa tcaggtcgcg ttcgaaggcg gcatcggcga gcaattcagg
     4021 cgcgaaaacg tcaaatacgt gccgaaattt tttgcggcgg cgattgtcgg cgaaaatcct
     4081 gcggatttcg gaatccagat gcagccgctt tcgacttata cgaaagaata gaccggaagt
     4141 cagaagttat gaggataaat ttctaccgtc ttccgtcttc cgtcttctaa tttctatctt
     4201 ctgaatttta atgactgaac tttggctcaa ttacaccgac gaaacgggcg aaaccagacg
     4261 aattctggtg gaaggcgaaa ggtttgtcat cggcaggcat tcggaaaatg atttgagcat
     4321 ttccgacgga agcctctcgc gccgacacgc gcaaatcgag cgcttcggcg atatttttgt
     4381 cgtttccgat ttgaattcga gcaacggaac gacgctcaac gaagcgccgc tggaagcgcc
     4441 ggtcgcgctg aaaaaaggcg ataaattcaa tctcggcggc ggtttcgaat ttgaagtcga
     4501 attgatttcc ggcgacgaaa acgccgacga tgcggctggc gcggcggcgc agaaagattc
     4561 ggaagaacag gctggcgcgg cgtcgggagc ggcggaaaaa gcttcggctg cctcgttttc
     4621 cgcagcgccg ggcggaaatt ccgtttcgat gaattttttt attctcgcgc cgcttttggg
     4681 cgtgtttgtt ttattgtttg tcggcggcgg tctgtttctg ctttccggca atggaaagga
     4741 agtcagcggc aatcaaggga attttattcg ttcggaaacg ccggagcgaa ttccgcgcgc
     4801 ggcaaacacg ggcgacgaaa cgccgacgcc gagcgtaaaa ccgagcgtcg gcgcatcgca
     4861 aacgccttca aattccacgg tcaattcagc gccgacgccg caaaattccg gcgagctgga
     4921 caaaatcgag cgcagcgctc tgcttttcac gcggcagatt gccattaacg acaacaatta
     4981 cgttttcgcg cgcgggcagt taaacgaaat caacaatcgc ctgaaaaatt ttaaaggctc
     5041 cgacgctctg cgcgacaatc tgaaagccgt caaacaaaac gccgcgcagt ttgaacaact
     5101 ggcgaaatcg aagggcttaa agccgcagtt tctggcgacc gcggcgctcg cccgcatcgg
     5161 caaccagcgc ggaaatccgc tgcagacggc acagtcgatg ctcaatgttt tgggcgaact
     5221 gaaaatctcg ctcggcaatg aactcgccga cgaaaatctt ttaatcatcg ccgcattcga
     5281 gcagggcgag cgcggcgagt ttcgcgcgat gcgcaatacg gtcgaggttt tgagcaaaca
     5341 gccgggcgtc gattccgacc gggcgcgaac catctggttc ctgcgcgaga agggaaaatt
     5401 gtcggacgcg caatacgaat tcgctttgcg gtttctggca atcggcgcga tttcgcaaaa
     5461 tcccgcggat tttaacgtcg agacagatgc cgttgttttt aattaacccg ggcaaaatta
     5521 aaattgataa taaataaggc agtcggaacg aggtgtaacg ggcagtttgc tatttactat
     5581 ttgggcttca cgatttacta ttaacctcga caaattttat gagcaaatat cgcaaggtgg
     5641 tctggaatga gggtatgctt ttaacgcccc agcatttcca gcagtgggac aattatcacg
     5701 aggaattgct gaactcgcgc gtgcgttcgg ttcagccata tgaatacggc gtgctggatt
     5761 tgcaggtgaa tcgcgaagcg attgccaacg gcagtttcca attgaccggc tgccgcgccg
     5821 ttttgcccga cggcttgatg ataaacgtcc cggacaccaa cgccgttccc gatttgcgcc
     5881 ccgtcggcga gcatttcaac cgcgaaaaag aaacgctggg cgttcacctg gcgattccgg
     5941 cgctgaaatt cggctcggcg aattttcagg gaaacggcgt caaaccgaac gccaatctgc
     6001 gatttttgca ggaaggctcg ctcatcaagg acgaaacgag cggcacgaac gaacagcctt
     6061 tagcctttgc gcgtcctaac ctgcgaatca ttttcgacga cgagctgcgc gacggtttta
     6121 cgtcgattaa aatcgccgag ctgcaaagaa cgacgaccgg gcagctgcag atttcggaaa
     6181 attatatccc gccggtttta aaagtttcgg cgtcggactg gctggtcaat atgctgcgcc
     6241 aactggtcga gattctgatt acgaaaagca gcagcttagg c
//
LOCUS       DASWNE010001037         2174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_69926, whole genome shotgun sequence.
ACCESSION   DASWNE010001037 DASWNE010000000
VERSION     DASWNE010001037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69926"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(277..1902)
                     /locus_tag="VGB68_19810"
     CDS             complement(277..1902)
                     /locus_tag="VGB68_19810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsI family protein"
                     /protein_id="HEY0051554.1"
                     /translation="MSDSVFNPKNFWKPVLTLAALAFLYATVLAKLAGDWWTDENYSH
                     GLLVPFVIGFIVWLEFDELKKVSGNPSLIFGGALILTALSMLLAGTLGAELFMQRASL
                     VLMLGGAVVYFFGVKILKLLLVPFALLLLSIPIPQIIFNKIAFPLQIWASQLAIWGIR
                     IFEVPSVRKGNVIELLPQGATQVVSLEVVEACSGIRSLMTLMTLALVLSYFMRAKKPD
                     AESGWLDCLKDFDFWRTLLLMFSAIPIAILTNAARVTATGVLTFYYGKQAAEGFTHDI
                     SGWLVYLVALGLLILVNFVLKKFQGRGGAETRGRGEKEILDDENEPFAASAPLRVSAS
                     LLIAVLLFGGFFINWFERRGEAAVERKTLSEIPAALGEWRQKGNDIRFSEQTESVLRT
                     SDYVMRDYYLADGSRANLYVGYYASQRTGATYHSPQNCLPGAGWEMKNPELVEIKTPR
                     GKTFTANRYIIQNGEYSEVLVYWYEGRGRAVASEYKDKIYTVFDSVARRRSDGAMIRV
                     MTPTAGAGDAESVQKAIDLSAQIADKLPEFVPE"
BASE COUNT          589 a          594 c          508 g          483 t
ORIGIN      
        1 gagagaaaat tcctaatcaa cgcaaagttt cagaaaaaac ctttttacaa ggaattaatt
       61 ttcgatttga ttgaaggcga cagccgatta tactccaact ttggcaggaa acaaacactt
      121 ttttgcattt acccaaagaa aaaattaaga caaaagtaaa aaggcaaaag gcgaaagtga
      181 aaatcaggaa ctcggagccg acactaatca ttatttgttg ccgtccgccg cccgactctt
      241 aattattgcc ttttcacttt cgccttccgt cttaatttat tcgggaacaa attcgggaag
      301 cttatcggca atctgcgccg ataaatcaat cgctttctgc acggattcgg cgtcgcccgc
      361 gcccgccgtc ggcgtcatta cgcgaatcat cgcgccgtcc gagcggcgac gggcgacgct
      421 atcgaaaacc gtgtagattt tgtccttgta ttcgctggcg acggcgcgac cgcgaccttc
      481 ataccagtaa acgagcactt ccgagtactc gccgttttgg ataatgtagc ggtttgccgt
      541 aaaagttttt ccgcgcggcg ttttgatttc gacaagctcc gggtttttca tttcccagcc
      601 cgcgccgggc agacagtttt gcggcgagtg ataagtcgcg cccgtgcgct gcgaagcgta
      661 ataaccgacg taaagattgg cgcgcgagcc gtccgccaga tagtaatcgc gcatcacgta
      721 atcgctcgtg cgcagaacgc tctcggtctg ctcgctgaag cgaatatcgt tgcccttctg
      781 ccgccattcg ccgagcgcgg cgggaatttc gctcaaagtt tttcgctcga ccgccgcttc
      841 gccgcgccgc tcgaaccaat tgataaaaaa accgccgaaa agcagaacgg cgattaaaag
      901 tgacgcggag acgcgcagag gcgcggacgc ggcgaaaggc tcgttttcat catcgagaat
      961 ttccttctct ccgcgcccgc gcgtctccgc gcctccgcgt ccctgaaatt tcttcaaaac
     1021 aaaattcacc agaatcagta aaccgagcgc gaccagataa accagccagc ccgagatgtc
     1081 gtgcgtaaag ccttccgccg cctgtttgcc gtaataaaac gtcaaaactc cggttgccgt
     1141 gacgcgcgcc gcgttggtca aaatcgcaat cggaatcgcc gaaaacatta aaagaagcgt
     1201 gcgccagaaa tcgaaatctt tcaggcaatc gagccagccg ctttccgcgt cgggcttttt
     1261 cgcccgcata aaatacgaca aaacgagcgc gagcgtcatt aaagtcatca ggctccgaat
     1321 gccgctgcag gcttcgacga cttccagcga tacgacctgc gtcgcgccct gcggcaaaag
     1381 ctcgatgacg tttcccttgc gcacggacgg aacttcgaaa atcctgatgc cccagattgc
     1441 cagctgcgaa gcccaaatct ggagcggaaa ggcgatttta ttaaatataa tctgcggaat
     1501 cggaatcgac agcagcagca gcgcgaacgg gacgagcaga agctttaaaa ttttaacgcc
     1561 gaagaaatag acgaccgcgc cgccgagcat caaaaccagc gaggcgcgct gcataaaaag
     1621 ctccgcgccg agcgttcccg ccagaagcat cgaaagcgcc gttaaaatca aagcgccgcc
     1681 gaagattaaa gaaggatttc cggaaacttt tttcagctcg tcaaattcca gccagacgat
     1741 aaagccgata acgaacggca cgagaagccc gtgcgaataa ttttcgtccg tccaccaatc
     1801 gccggcgagc ttcgccaaaa ccgtcgcgta aagaaaagcc agcgccgcca gcgtcaaaac
     1861 cggcttccag aaatttttgg gattgaaaac agaatcgctc attcaactac gagtaaggaa
     1921 aaagaaagaa tcgcggacat tttccgcatt cgtcgaaaat tatactttct ttaaagaaaa
     1981 agattcaacg taaaaataaa taaatgcgcc cgccggacgg cggctcttca tcctcgcttc
     2041 cgtctttttt tattcttttt gatacctttt gcccgcgctt tccgctatta ccggagaaga
     2101 cttttcttca atcttttata ttttatatcc gataagtgag aaagcaatta tgtctattat
     2161 tcgaaaaatc gcct
//
LOCUS       DASWNE010001038         5470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_246345, whole genome shotgun sequence.
ACCESSION   DASWNE010001038 DASWNE010000000
VERSION     DASWNE010001038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5470
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246345"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VGB68_19815"
     CDS             <1..367
                     /locus_tag="VGB68_19815"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY0051555.1"
                     /translation="FWNLRANGGILSTVGDLYKWHLALEGEKILSKEAKEKYFAPHIA
                     EQPEAVSFYGYGWVNQKTPRGTTLIWHDGGNGFFYADFRRYIDEKVVYIVATNSRISS
                     FRKEYAQVLRSIFSADASK"
     gene            complement(497..1531)
                     /locus_tag="VGB68_19820"
     CDS             complement(497..1531)
                     /locus_tag="VGB68_19820"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY0051556.1"
                     /translation="MNKESEFVVLEETGKLGVRHLKRFWARTLAARDGKIFDNREDDR
                     RFDNLILNGLSLSLEETLCYLSRFAPGFDEFENWILTKNGGAIDELQIERLNCVLSNQ
                     PYGERLRNSLKQIEDSADVLTDEDLDFWEENGYVVVREAVSESQARATETAVWEFLEM
                     EPSDSASWYEKPIGKGIMMDFYHHRTLLENRNSPRIQKAFAQLWKTADLWATTDRTSF
                     NPPETESYSFQGPRLHWDMSLEPPFRFGTQGLLYLCDTSAEQGAFCCVPKFHRNLENW
                     LKDLPENTDPRQVNLESEAVPIAASAGDFVIWHQALPHGSSPNRGKYPRIVQYLNMYP
                     SEFRENEKWK"
     gene            1973..2230
                     /locus_tag="VGB68_19825"
     CDS             1973..2230
                     /locus_tag="VGB68_19825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051557.1"
                     /translation="MPDCKHYGGITREELKHLRKDLANEGVMVPEGDDVEFKGLFGVY
                     LRATYDEAKETLKICITKKPFFVPESEVWKIVDAGTAPYVG"
     gene            complement(2332..2823)
                     /locus_tag="VGB68_19830"
     CDS             complement(2332..2823)
                     /locus_tag="VGB68_19830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051558.1"
                     /translation="MFELKPISKEAVESALEKAKHYRLLNEPGAAESICLDILRVEPD
                     NQEALVTLVLAMSDRFVRGYAIGDAQIQDYLSQIRDDYERAYYTGIIYERRAKANLSK
                     GSLGAESTAYEFFRQAMNWFEKAEAIRPPKNDDALLRWNGCARIVMRNKLEPREMDAD
                     FLE"
     gene            complement(2854..3606)
                     /locus_tag="VGB68_19835"
     CDS             complement(2854..3606)
                     /locus_tag="VGB68_19835"
                     /inference="COORDINATES: protein motif:HMM:NF009052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEY0051559.1"
                     /translation="MAKATFKIWRGDKDSGALKSYETEITEGMVVLDAVLQIQGEQAG
                     DLACRWNCKAGKCGSCSAEINGMPKLMCMTRMNTLPLDNPITVEPIRAFPLKKDLVTD
                     VSWNYEVKKRIKQFKPRPPDAPDGTWRMQQEDIDRVQEFRKCIECFLCQDVCHVLRDH
                     HKHEEFIGPRFLVYAAALEMHPLDTENRLEELKDEFGIGYCNITKCCTKVCPEHITIT
                     DNAIIPLKERVVDEFYDPIKKLVRIFRPKKKK"
     gene            complement(3636..5447)
                     /locus_tag="VGB68_19840"
     CDS             complement(3636..5447)
                     /locus_tag="VGB68_19840"
                     /inference="COORDINATES: protein motif:HMM:NF005866.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HEY0051560.1"
                     /translation="MAEYQTFEHDVLVIGAGGAGLRAAIEASAAGISVGLVCKSLLGK
                     AHTVMAEGGMAAAMANVDDRDNWKVHFSDTMRGGQYVNNWRMAELHAREAPDRVRELE
                     AWGAVFDRTKDGRILQRNFGGHKYPRLAHVGDRTGLEMIRTLQDHGIHQGIEVYMEVT
                     VVALLKDGDRVVGAFAYEREHGRFRIFKAKAVILATGGIGRAYKITSNSWEGTGDGHS
                     LAFDAGAELIDMEFIQFHPTGMVYPPSVRGILVTEGVRGEGGILRNSEGRRFMFDDIP
                     DNYKDQTADTPEEGWRYTQGDKNARRPPELLTRDHVARCIVREVKAGRGTPNGGVYLD
                     IAWIKEKISNAPEHIRKKLPSMYHQFKQLADLDITTTPMEVGPTTHYIMGGVRVDADT
                     QMSTVPGLFAAGECAAGINGANRLGGNSLSDLLVFGKRAGEYAAKFARENDFGNIDNG
                     QIQEKVRWSLEAFDRTNGENPFTIQHELQEMMQDLVGIVRTEDEMQRALIGIKELKER
                     REKISLTGNIDFNPSWHTALDLHNLLNVSEAITLAAIERKESRGGQFRDDYPAKDPEF
                     AKFNFTIKKGADGAMQIARVPIPEMPDELKRIIEEMG"
BASE COUNT         1195 a         1492 c         1345 g         1438 t
ORIGIN      
        1 tttctggaat ttacgcgcca acggcggaat tctttcgacc gtcggcgatt tatacaagtg
       61 gcatcttgcg ctcgaaggcg aaaaaattct ctcgaaagag gcaaaggaaa aatacttcgc
      121 gccgcacatt gccgaacagc ccgaagccgt ttcgttttac ggttacggct gggtaaatca
      181 gaaaacaccg cgcggcacga ctttgatttg gcacgacggc ggcaacgggt tcttttacgc
      241 tgattttcgt cgttacattg acgaaaaggt agtttatatc gtcgcaacca attcacggat
      301 ttcttctttc cgaaaggaat atgctcaagt tttgcggagt atttttagcg ccgatgcgag
      361 taaataaagc cgtgccgaac aagccgcgcg cgcgccggag tttctcgaca gcgcgagcaa
      421 gcatttaatc acatctgttt tttgttggag cgcctgacat tttttcggtc ttccgcagct
      481 ttttgcggct gattttctat ttccactttt cgttttccct gaattccgaa ggatacatat
      541 tgagatattg gacgatgcgc ggatatttcc ctcggttcgg actgctgccg tgcggaagcg
      601 cctgatgcca gatgacgaaa tcgccggcgc tagcggcaat cgggacggct tccgattcga
      661 gattgacttg tcgcgggtca gtattttcgg gcaaatcctt gagccagttt tccagattcc
      721 ggtgaaattt cggaacgcag caaaacgcgc cttgttcggc ggatgtatca cacagataaa
      781 gcaagccctg cgtgccgaaa cgaaacggcg gttcgaggct catatcccaa tgaaggcgcg
      841 gaccttgaaa gctgtaactt tcggtctcgg gcggattaaa gctcgtgcgg tcggtcgtcg
      901 cccagaggtc ggcggttttc caaagctggg cgaaagcctt ttgaatacgc ggcgagtttc
      961 ggttttcgag cagcgtccga tgatgataga aatccatcat aattcccttg ccgattggtt
     1021 tttcatacca cgacgccgaa tcgctcggct ccatttcgag aaattcccag accgccgttt
     1081 ccgtcgcccg cgcctggctt tcggaaacgg cttcgcggac gacgacatag ccgttttctt
     1141 cccaaaaatc caaatcttca tcggttaaaa catccgcgct gtcctcgatt tgtttgagcg
     1201 aatttcgcag cctctcgccg taaggctgat tcgacaaaac acagttgagg cgttcgattt
     1261 gcagttcgtc aatcgcgccg ccgtttttcg tcagaatcca attttcaaac tcgtcaaaac
     1321 cgggcgcaaa ccgcgacaga taacagagcg tttcttcgag cgatagactc aagccgttca
     1381 aaatcagatt gtcgaaacgc cggtcgtctt cgcgattatc aaagattttc ccgtcacgag
     1441 cggcgagcgt ccgcgcccaa aatcttttga gatgtctgac gccgagtttt ccggtctctt
     1501 caaggacgac gaattcagat tctttattca ttttaccttt ctgtttatca acaaatttac
     1561 ggctctctga tttgaaagat tgatagctca aatttaattt cggaatccgg cactggagcg
     1621 gcgtgccgtt ccagatatat tgtcctttca aaaacacatt tgcaagaagc ggcggcagcg
     1681 ggctcgaatc ttttacgaat gataaactat ttgaatcgag caatgtgacg gcgactgaaa
     1741 aatgccgggt tatgaaggta tccggaatat atcctgcgag agccggtaaa gctcaggatt
     1801 ataaattcag ccggctcaaa agcgctcctt ttccggacaa ttaaaacaga gtaagatgtg
     1861 tcctgtaaat aaaagacccg tttagggcaa ttctcgcaat tgcctttttc tattccgttg
     1921 acgattcgct gttataatcg aaattggata caaattcact aaggagtttt ctatgcctga
     1981 ctgcaaacat tacggcggta ttaccagaga agagctaaag cacctgcgga aagatttggc
     2041 aaacgagggc gtgatggttc ccgaaggcga cgacgtcgaa tttaaaggac ttttcggagt
     2101 ttatctgcga gcgacttatg acgaggctaa ggaaaccctg aaaatctgca ttaccaaaaa
     2161 accgttcttc gttcccgaat ccgaagtctg gaagattgtc gatgccggaa ccgcgcctta
     2221 tgtcggttga aagtgaaaga gtgaaaaagt gaaaaagttt ggctgaatta ccgctcaaat
     2281 tactttttca ctttttcact ctttcacttt ttcacgcttt tcactttttc gctattccag
     2341 aaaatcggcg tccatctcgc gcggctcgag tttatttcgc atcacgattc gcgcgcagcc
     2401 gttccagcgc agcagggcat cgtcgttttt cggcgggcgg atggcttcgg ctttctcgaa
     2461 ccagttcatc gcctgccgga aaaattcgta agccgtcgat tccgcgccga gcgagccttt
     2521 gctgagatta gccttggcgc ggcgctcgta aatgattccg gtgtaataag cgcgctcgta
     2581 atcgtcgcga atctgcgata aataatcctg aatctgcgcg tcgccgatgg catagcctct
     2641 gacaaagcgg tcgctcatcg ccagcacgag cgtgaccagc gcttcctgat tatcgggctc
     2701 gacgcgcaga atatcgaggc agatgctttc cgccgcgccc ggttcgttga gcaggcgata
     2761 atgcttcgcc ttttcgagcg ccgactcgac cgcttctttg gagataggtt ttaactcaaa
     2821 cataatcgct ccgaatcaaa catcgtatcg ttattatttc tttttcttcg gtcggaaaat
     2881 ccgcaccagt tttttaatcg ggtcgtaaaa ttcgtcaacg acgcgctctt tgagcgggat
     2941 aatcgcgtta tcggttatcg tgatgtgttc cggacaaacc tttgtgcagc actttgtgat
     3001 attgcaatag ccgatgccga attcgtcctt taattcttcc agacgatttt ccgtatcgag
     3061 cggatgcatc tccagcgccg ccgcgtaaac cagaaatcgc ggaccgataa attcctcgtg
     3121 tttatgatgg tcgcgcaaaa cgtggcaaac gtcctgacac aaaaaacact cgatgcattt
     3181 gcgaaattcc tgcacgcggt cgatgtcttc ctgctgcata cgccaagttc cgtccggcgc
     3241 gtccggcggg cgcggcttaa attgtttgat tcgttttttg acctcgtaat tccacgaaac
     3301 gtccgtcacc aaatcttttt tcaatggaaa cgcgcgtatc ggctcgaccg ttatcggatt
     3361 atcgagcggc agagtgttca tccgcgtcat acacatcagc ttcggcatcc cgttgatttc
     3421 cgccgagcac gagccgcatt tccctgcctt gcagttccag cggcaagcca aatcgccggc
     3481 ttgctcgccc tgaatttgca gaacggcgtc gagcacgacc attccctcgg tgatttccgt
     3541 ttcatacgat ttcaacgcgc cgctgtcttt atcgccgcgc cagattttga atgtcgcttt
     3601 tgccatttac ttttgccttt ttcctttttg cttaactaac ccatttcctc gataatccgt
     3661 ttcaattcgt caggcatttc gggaatcggc acgcgcgcga tctgcatcgc gccgtccgcg
     3721 ccttttttaa tcgtgaaatt gaatttggca aattccgggt ctttcgccgg gtaatcgtcg
     3781 cgaaactgcc cgccgcgact ttcctttctt tcaatcgccg ccagcgtaat cgcttcggaa
     3841 acatttagta aattatgcaa atcgagcgcc gtgtgccagc tcggattaaa atcgatgttg
     3901 cccgtcaggc tgattttctc gcgccgctct ttaagctctt taattccgat tagagcgcgc
     3961 tgcatctcgt cttcggttct gacaatgccg accaaatcct gcatcatttc ctgcaattcg
     4021 tgttgaatgg tgaaaggatt ttcgccgttc gtgcggtcga atgcttcgag cgaccagcgc
     4081 actttttcct gaatctgccc gttgtcgatg tttccaaaat cgttttcgcg cgcaaatttc
     4141 gccgcgtatt ctccggcgcg cttgccgaac accagcaaat ccgagagcga atttccgccc
     4201 agacgattcg cgccgttgat tcccgccgcg cattcaccgg cggcaaaaag ccccggaacg
     4261 gtggacattt gcgtatcggc atcgacgcgc acgccgccca ttatataatg cgtcgtcggt
     4321 ccgacttcca tcggcgtcgt cgtaatgtcc aaatccgcga gctgtttaaa ctggtgatac
     4381 atactcggca gttttttcct gatgtgctcg ggcgcgttgg aaattttttc cttaatccag
     4441 gcgatgtcca gataaacgcc gccgttcggc gttccgcgcc cggctttgac ctcgcgcacg
     4501 atacagcgcg ccacgtggtc gcgcgttaaa agctcgggcg ggcgacgcgc gtttttgtcg
     4561 ccctgcgtgt agcgccagcc ttcttcgggc gtatcggcgg tctggtcttt gtaattgtcg
     4621 ggaatgtcgt cgaacataaa gcgacgacct tcgctgtttc gcaaaattcc gccttcgccg
     4681 cgcacgcctt cggtgacgag aatgccgcgg acgctcggcg gataaaccat tccggtcggg
     4741 tgaaactgga tgaattccat atcaatcaat tccgcgcccg catcgaaagc cagcgaatgc
     4801 ccgtcgcccg ttccttccca gctgttgctc gtgattttat acgcccgacc gattccgccg
     4861 gtcgccaaaa taacggcttt cgccttaaaa atccgaaatc tcccgtgctc gcgctcgtag
     4921 gcaaacgcgc cgaccacgcg gtcgccgtct ttgagcagcg caacgacggt gacttccata
     4981 taaacttcga tgccctgatg aatgccgtgg tcttgaaggg tacgaatcat ctccaaaccc
     5041 gtgcggtcgc cgacgtgcgc cagacgcggg tatttgtgcc cgccgaagtt tctttgcaga
     5101 atgcgcccgt ctttggttct atcgaaaacc gcgccccagg cttccagctc gcgcacgcgg
     5161 tcgggcgctt cccttgcgtg aagctcagcc attcgccagt tgttgacgta ttgaccgccg
     5221 cgcatcgtgt cggaaaaatg cactttccag ttgtcgcggt cgtcgacgtt tgccatcgcc
     5281 gccgccattc cgccttccgc catcacggta tgcgcttttc cgagcagcga tttgcagaca
     5341 agcccgaccg aaatgcccgc cgccgacgct tcgattgccg cgcgaagtcc cgcgccgccg
     5401 gctccgatta ccagaacgtc gtgttcgaat gtttgatatt cagccataat ttaaaggata
     5461 aaggcgaaag
//
LOCUS       DASWNE010001039         2211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_600075, whole genome shotgun sequence.
ACCESSION   DASWNE010001039 DASWNE010000000
VERSION     DASWNE010001039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600075"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..1786
                     /locus_tag="VGB68_19845"
     CDS             74..1786
                     /locus_tag="VGB68_19845"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF017038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEY0051561.1"
                     /translation="MIEREQLPMDVVFVGAGPANLAAALHLKNLIDRHDEEIASGKRT
                     GKPLGELEIAIIEKGSFVGAHILSGAVLDPRTFKELMPDFIEQGCPVDAPVTDDAFWY
                     LTEKSKIAAPITPPPLKNHGYYIASLSRICEWMGEKCEEAGINIFPEFPAAEVLYDET
                     DAVIGVRTGDKGIGKDGKPKANFEPGVDLLARVTVLGEGSRGSLTKQLTTRLGLNKGK
                     EPTVYSLGVKELWEVPAENFAEGRIIHTLGFPSDAKTYGGGWIYGMKNNIISIGYVTG
                     LDYLDPLIDPHAEFQKFKTHPEIASILAGGKMIKYGAKTINAGGWHTMPQLYADGVLL
                     VGDCASFLNGQRIKGIHTAMKSGMLAAETILEALVKDDYTTETLRHYEQKVNGSWLYD
                     ELHAVRNFHGSFQKGRWSALVNSGLQFLSGGFAWGFIDRESHQAGHERMQKLEKYDYE
                     GDNAAQRYPDLKFDKQLTFDKLTDVYHGAVAHDEDQPAHLHVLDTEICATRCAEEYGN
                     PCQRFCPAAVYEMEHSDKTGRRELKINFSNCVHCKTCDISDPYQIINWVTPEGGGGPN
                     YKGM"
     gene            1956..2189
                     /locus_tag="VGB68_19850"
     CDS             1956..2189
                     /locus_tag="VGB68_19850"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEY0051562.1"
                     /translation="MSIPNNDEVEGKFDQVKGKVKGTVGNLVGDKQMEVEGEADETQG
                     ETQESWGKVKRGVGDAVDAVGDAISNAGDKINR"
BASE COUNT          602 a          542 c          616 g          451 t
ORIGIN      
        1 tttccgaaaa cacaaaaaaa atttgctata ctgaaaaaaa actgaatgac cattcatttt
       61 aaagaggcaa ataatgattg aacgtgaaca actaccgatg gacgtggttt ttgtcggcgc
      121 gggaccggca aatctggcgg ctgccctgca tctgaaaaat ttaatcgacc ggcacgacga
      181 agaaatcgcg agcggcaaac ggacaggcaa accgctcggc gaactggaaa tcgcgattat
      241 cgaaaaaggc tcgttcgtcg gcgcgcacat tttgtcgggc gcggtgctcg acccgcgaac
      301 ttttaaagag ctgatgccgg attttatcga acagggctgt cccgtcgacg cgccggtcac
      361 cgacgacgcc ttctggtatc tgaccgaaaa aagcaaaatc gccgcgccca ttacgccgcc
      421 gccgctgaaa aatcacggtt actacatcgc atctttgagc agaatctgcg aatggatggg
      481 cgaaaaatgc gaagaagcgg gcatcaatat ttttcccgaa tttcccgccg ccgaagtttt
      541 gtatgacgaa acggacgccg tcatcggcgt gcgcacgggc gacaaaggca tcggaaagga
      601 cggcaagccg aaagctaatt tcgagccggg cgttgacctt ctggcgcgcg tcacggttct
      661 cggcgaaggc tcgcgcggct cgctgacaaa gcagttgacg acgcgcctgg gcttgaacaa
      721 aggcaaagag ccgaccgttt attctctggg cgtcaaagag ctttgggaag ttccggcgga
      781 aaattttgcg gaaggtcgaa tcattcacac gctcggtttt ccgtcggacg cgaaaaccta
      841 cggcggcggc tggatttacg ggatgaaaaa caacattatc agcatcggct atgttacggg
      901 attggattat ttagacccgc tcatcgaccc gcacgccgag tttcagaaat tcaaaacgca
      961 tccggaaatc gcctcgattc tggcgggcgg aaagatgatt aaatacggcg cgaaaaccat
     1021 caacgcgggc ggctggcaca cgatgccgca gctttacgcg gacggcgttc tgctggtcgg
     1081 cgactgcgct tctttcctga acggtcagcg catcaaaggc atccacacgg cgatgaaatc
     1141 gggaatgctc gcggcggaga cgattttaga ggcgctcgta aaagacgatt acacgaccga
     1201 aacgctccgg cattacgagc agaaagtaaa cggttcgtgg ctttacgacg agcttcacgc
     1261 cgttcgcaat tttcacggct cgtttcagaa aggtcgctgg tcggcgctcg tcaacagcgg
     1321 tctgcagttt ttgagcggcg gtttcgcgtg gggatttatc gaccgggaat cgcatcaagc
     1381 cgggcacgaa agaatgcaga agctcgaaaa atacgattac gagggcgaca acgccgcgca
     1441 gcgttacccg gatttgaaat tcgataaaca gttgactttc gataagctga ccgacgttta
     1501 tcacggcgcg gtcgcgcacg acgaagacca gcccgcgcat ctgcacgttc tggatacgga
     1561 aatctgcgcg acgcgctgcg ccgaagaata cggcaacccg tgccagcgtt tctgcccggc
     1621 ggcggtttac gaaatggaac acagcgacaa aaccggcagg cgcgagctga aaatcaattt
     1681 ttcaaactgc gttcactgca aaacgtgcga catttccgac ccgtatcaaa ttatcaactg
     1741 ggtgacgccc gaaggcggcg gcggtccgaa ttacaaggga atgtaaatta atgcgaaacg
     1801 ccaaatgcag agtgcaaaat taagaaataa ttatattttg cactttgcgt tttgcttttt
     1861 gcattttcga agggaatata atttgctctg acttcctttt tgtgtggttt tgtaacaaaa
     1921 cattgcacaa attttattat aaggggaaaa atataatgtc aattccgaat aacgacgaag
     1981 tcgaaggcaa atttgaccag gtgaaaggaa aagtaaaagg cacggtcggc aatctggtcg
     2041 gcgacaaaca gatggaagtc gagggcgaag ccgacgagac gcaaggcgag actcaggaat
     2101 cctggggcaa agtcaagcgc ggtgtcggcg atgcggtcga tgccgtcggc gacgcgataa
     2161 gcaatgccgg cgacaaaatc aaccggtaat tcagaggggc gaaagatttc g
//
LOCUS       DASWNE010001040         2657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_386569, whole genome shotgun sequence.
ACCESSION   DASWNE010001040 DASWNE010000000
VERSION     DASWNE010001040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386569"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2506)
                     /locus_tag="VGB68_19855"
     CDS             complement(<1..2506)
                     /locus_tag="VGB68_19855"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0051563.1"
                     /translation="MTDERWQQIEAILHSALDIESSAERERFIADACFGDDELRAAVE
                     RLIAADADAGSFIESPAWTDSGMLGTVLKSSFKNALGKPPFDENENSSADADDLIGKK
                     IGVFELEKELGRGGMGAVFLAARADGEFRQRVAVKLIKRGMDTDFIVKRFRHERQILA
                     ALNHPNIAALIDGGTTPDGLPYFVMEYVEGEPLYRFCDERKLSTTERLRLFGRLCDAV
                     EAAHQLKVVHRDLKPSNVLVKADGTPKLLDFGIAKILDTDLASATLEPTATAMRLMTP
                     EYACPEQVSGFPVTFASDIYSLGVVLYELLTGHRPYRFKSRAPHEIARVICEEEPMRP
                     STGITREDNLLPTAAGKSVTLSDVYLSRGAQDIESLQKEISGEIEKVILKCLRKNPDE
                     RYRRAAELAEDIARFLSGEPIRAETLIPGVESKNTETFSAAEQGDFFRNVKYKFGLAI
                     ISAILILGALAFVFVLNSEKTGSQIESQSQPDSKRSVAILPFKNETSNAENDFLCDGL
                     SESLINRLSYLPEIQVMSRSAAFKYKGAQIAPEQAGREMNVATVLSGKLSKSNDKIAI
                     FLELIDVSDGSQIWSFQYGGKESELVFLQNKMAGDVAAQFKTGKASEQKQIVSRGYTE
                     NPEAFELYMKGEFERQKATAEGSRKSIEFYRRALELDANYALAYQGLALSCRLAPAYR
                     TLEPQEAYPMAKEAAMKALAIDPTLGAAYIPLASIKFAYDWDFAGAENEYRQAIRLAP
                     NNPEAHSSYSTFLVAMGRTEEALSELKIARQFDPNSPNYTSSIAWALYIAGRFDEAEA
                     ELKQVLKRDSNYARAFTNLSEIYMEKGKFDEAREALQ"
BASE COUNT          594 a          702 c          672 g          689 t
ORIGIN      
        1 tctgaagcgc ttcgcgcgct tcgtcgaatt tgcccttttc catatagatt tcgctgaggt
       61 ttgtgaaggc gcgggcataa ttcgaatcgc gcttgagaac ttgtttgagc tccgcctccg
      121 cttcgtcgaa acgcccggca atgtataaag cccaggcgat actcgatgta taattcggcg
      181 aattggggtc gaactgccgg gcgattttca attcgcttaa ggcttcttcg gtgcgtccca
      241 tcgcgaccag aaaagttgag taggaagaat gagcttctgg attgttcggc gccagcctga
      301 tcgcttgccg gtattcgttt tcagcgcccg caaaatccca gtcgtaagca aattttatcg
      361 aagccagagg aatgtaagcc gccccgagcg tcgggtcgat tgccagagct ttcatcgccg
      421 cctctttcgc catcggatag gcttcctgcg gctcaagcgt gcgataggcg ggcgcgagcc
      481 ggcaggaaag cgccaagccc tggtaagcca gagcgtaatt tgcgtccagt tccaaagccc
      541 gccggtaaaa ttcgattgat ttacggctgc cttcggcggt tgctttttgc cgctcgaatt
      601 cgcctttcat gtataattcg aaagcctcgg ggttttccgt atagccgcgc gagacgattt
      661 gtttctgctc gctcgctttg ccggttttga attgcgccgc gacatcgccc gccattttat
      721 tttggaggaa aaccagttcc gattctttac caccgtactg aaaagaccaa atctgcgagc
      781 cgtcggaaac gtcgatcaat tcgaggaaaa tcgctatctt gtcattgctt ttggaaagtt
      841 ttcccgataa aaccgtcgcc acgttcatct ctctgcccgc ttgttccggg gcaatctgcg
      901 ctcccttgta tttgaaggcg gcggaacgcg acataacttg aatttcgggc agataagaca
      961 accgattgat gagactttcg ctgagcccgt cgcataaaaa atcgttttcc gcgtttgaag
     1021 tttcgttttt aaacggcaaa atcgctaccg agcgtttcga gtcaggctga ctttgagatt
     1081 cgatttgaga gccggttttt tctgaattta aaacgaagac gaaagccagc gcgcccaaaa
     1141 tcaaaatcgc tgagattatt gccagcccga atttatattt gacgtttcta aaaaaatctc
     1201 cctgctcggc tgctgaaaac gtctccgtat ttttcgattc gacgcccggt attaatgttt
     1261 cggcacgaat cggctcgccg ctgaggaagc gcgcaatatc ttcggcaagc tcggcggcgc
     1321 gccggtaacg ttcgtccgga ttttttcgca ggcatttcaa aatcactttt tcgatttcgc
     1381 cggagatttc tttttgcagt gattcgatgt cctgagcgcc gcgagacagg taaacgtcgg
     1441 agagcgtgac ggatttgcct gcggcggtcg gaagcagatt gtcttcgcgc gtgatgcccg
     1501 tgctcggtcg catcggttct tcttcgcata taacgcgggc gatttcgtgc ggcgcgcggc
     1561 tcttgaaacg atacgggcgg tgtccggtca aaagctcgta aagcaccacc ccaaggctgt
     1621 aaatatccga agcaaatgtt accggaaagc cggaaacttg ttcggggcag gcgtattccg
     1681 gcgtcatcag gcgcattgcc gtcgcggtcg gctctaaggt cgcggaagcc aaatccgtat
     1741 cgagaatttt ggcgatgccg aaatcaagca gcttcggcgt cccgtccgct ttcactaaaa
     1801 cgttcgacgg cttcaaatcg cggtgaacga ctttaagctg atgcgccgct tcgacggcgt
     1861 cgcacaaacg cccgaataat cgcaatcttt cggtcgtgct cagttttcgt tcgtcacaaa
     1921 aacgataaag cggctcgccc tcgacgtatt ccatgacaaa atacggtaag ccgtccggtg
     1981 tcgtcccgcc gtcaattaag gcggcgatat tcggatgatt aagcgcggct aaaatctgtc
     2041 gttcgtggcg aaaccgcttg acgataaaat ccgtgtccat tccgcgcttg attaatttta
     2101 ccgcgactct ctggcgaaat tcgccgtccg ctctggctgc cagaaaaact gcgcccattc
     2161 cgccgcgccc taattctttt tcgagctcaa aaacgccgat ttttttgccg attaaatcgt
     2221 ctgcgtcagc cgatgaattt tcgttctcat cgaacggtgg tttgccgagc gcatttttaa
     2281 aactgctttt gagaacggtg ccgagcattc cgctgtccgt ccaggcaggc gattcgataa
     2341 aactcccggc atcggcatcg gcggcaatca atcgttcgac cgccgctcgc agctcatcgt
     2401 cgccgaaaca ggcatcagct ataaagcgct cgcgttcggc gctcgattca atgtcgagcg
     2461 ccgaatgcag gatcgcttca atttgttgcc aacgctcgtc tgtcacttac atttaacggt
     2521 atttgccgga aagcctgaaa atcaagttgg attttacttt tcaaatacgt ttaaaagcaa
     2581 gatgagaatt tcaaactttg tcgcgcgcga taatcgaaat catcaaattc ttaaaacagc
     2641 cggctattca gagcaat
//
LOCUS       DASWNE010001041         4658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_528794, whole genome shotgun sequence.
ACCESSION   DASWNE010001041 DASWNE010000000
VERSION     DASWNE010001041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4658
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528794"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VGB68_19860"
     CDS             <1..187
                     /locus_tag="VGB68_19860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051564.1"
                     /translation="ALKQLALIEPTFIENGEREFPAETVEEIFTARKNSYTFFVTTVK
                     EEKLPAEIQQTAKVLRY"
     gene            191..733
                     /locus_tag="VGB68_19865"
     CDS             191..733
                     /locus_tag="VGB68_19865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051565.1"
                     /translation="MRKITCALAAIFTFFLGAAIYSAFLSLAVDKTNSLIQPLENSSA
                     PIVSLSEATSNPEFYKISDIRVRANFYVENDGDNVGIFGIGKSGDYKFAGIEFRNDRG
                     ILIREELTAETRRLIERLKEKDSKDLKAFAEVEILGELHEQSPQHYCLKPRFYMAVKE
                     IRQVSPLKTIDFAGKSFNCK"
     gene            949..1524
                     /locus_tag="VGB68_19870"
     CDS             949..1524
                     /locus_tag="VGB68_19870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0051566.1"
                     /translation="MTDVETLMDAMSEQAAVTAKIEVGFDEAFTLHHRTVFRTARAVV
                     RDAGLAEDITQEVFMRLYNNLDSIKDDEMLRPWLIRVTLNLSRNQLRSNFRSNVREET
                     YVKEADEAGSFTIDHETELERRQQAEEVHRALNKIKEPLRSCLVLKQQGLSYKEIAES
                     LSLNETSIGQFVARARKEFMRFYGKIGKEEA"
     gene            1524..2438
                     /locus_tag="VGB68_19875"
     CDS             1524..2438
                     /locus_tag="VGB68_19875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051567.1"
                     /translation="MDRCFDEGIVQAFLDGELASDLSENVACHVALCEKCAMMLAAAE
                     EESSFAFAALDAEFNALVPTERIRTRLFETIATERKPFWQKIFGKGFRWTNPSVAAFA
                     SLILVVGLFATLWIARENSMKEGQIAGIEKPQSTVAAPLSPTKSVDSTPAVNPPVPFE
                     YVEPVAPQIRQASYKPKNENRFEAQKAVYKTGNNIVRPPIDRAPAETNYNAISGEESY
                     LKTIATLQQSVDNRKDELMKPSMRVAFEKDMAVVDDAIKKMQREVRKNPKNEAAREVL
                     RSSYQNKIDLLNSVTEKSELMASLNSGK"
     gene            2447..3523
                     /locus_tag="VGB68_19880"
     CDS             2447..3523
                     /locus_tag="VGB68_19880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051568.1"
                     /translation="MKKEKGKFFIRGYEASQKAILLSFAFFLLFSASEVFSQIDKNEP
                     KVIVVVPPAVQPTPPPRPSIPRKRRADNESTAAAEKSIRTEAKVNVSLCVSEGNVRIN
                     GWERNEIRAFVDGGSKVGFKVMQKKQNPVWVMVLGFDPLTDKEPGLDECLSGANIELD
                     VPRGAIINMKSRASQISVESIARVKIENIGGDISLNNIAGGIDAGTYQGDVMVEKSAG
                     AMSLFATTGNIVVFDVEPSEVGDSFRAKTRNGTVTLQSVGHSLVEANSTSGSIRFNGE
                     FTGGGQYTFVTNNGTILLSVPAESSCRINANYGLGAFQSDIALKDVQKTSGARVQKLT
                     ALLGAGDANLTLTTYSGAIRIKKK"
     gene            3776..>4658
                     /locus_tag="VGB68_19885"
     CDS             3776..>4658
                     /locus_tag="VGB68_19885"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEY0051569.1"
                     /translation="MSWVISLVLAGLMITPDSNLSINADNNFVNANAPTVVRLDETER
                     FEQTYPLNAGGRVSVSNVNGSIIVEAWDRNEVRLEAVKTADTKEHLAEVQIKIDARQD
                     SFSVETDYGSWKRNNDGVWKNKNYGKLEVQYRLQVPRGAVLDEVETVNGSVTISNMTN
                     STKASAVNGNVKATNLRGTARISTVNGTTEADFDSLAGTGQITLDTVNGKVLLTIPSD
                     ANATVRADTLNGQIYNDFGLPVRKGEYVGRDLYGKIGSGALNIKLSSVNGELSIRRKQ
                     DGKSVSPATNLLPQKNKA"
BASE COUNT         1282 a         1104 c         1227 g         1045 t
ORIGIN      
        1 cgctctaaag cagctcgctt tgattgagcc gacttttatc gaaaacggcg agcgggaatt
       61 tcccgccgag accgtcgagg aaatctttac cgccaggaaa aatagctata catttttcgt
      121 gacgaccgta aaagaagaaa aactgcccgc cgagattcag cagacggcga aggttttgag
      181 gtattaggtt atgagaaaaa tcacctgtgc tttagccgcg atttttacat tttttctcgg
      241 cgcggcgatt tattctgctt ttctgagctt ggcggttgat aaaacgaatt ccttgattca
      301 gccgctcgaa aattccagcg ctccgattgt ttctctttcg gaagcgacga gtaatccgga
      361 gttttacaaa attagcgata ttcgcgtgcg ggcgaatttc tatgtggaaa atgacggcga
      421 taacgtcggc attttcggca tcgggaaaag cggcgattac aagtttgcgg gcatagaatt
      481 ccggaacgac cggggaattt taatcagaga agaattaacg gctgagacgc ggcggctgat
      541 agagcggctc aaagaaaaag actcgaaaga tttaaaggca tttgccgaag tcgaaatact
      601 tggagaactg cacgaacagt cgccgcagca ttattgcctc aaacctcgat tttatatggc
      661 tgtaaaagaa atcagacagg tttcgccgct caagacaatc gattttgccg gaaagagttt
      721 taattgtaaa tagtgaatat tacccgtgcg aatttacggg cggtttttat cgctcaggat
      781 aattttcgac aaatccgctt agccgaaaca aaaaaaccgg aaaaatcgtt ttcaaaacgg
      841 ggagaatgat tttcgcgtct tttctgtttt ttcggttgaa tttaaaaatt cctgtcacaa
      901 aattcacaag cgcggtgttt tttatacgga agcagttttg atgttaaaat gacggacgtg
      961 gaaacattga tggacgcgat gtccgagcag gcagcagtaa cagcgaaaat tgaggtcggg
     1021 ttcgatgagg ctttcacgct tcatcaccgg actgtgtttc gcactgcgcg cgcggtcgtg
     1081 cgcgacgcgg gacttgcgga agacatcacg caggaagttt ttatgcgtct ttacaacaat
     1141 ctggacagca tcaaagacga cgaaatgctg cgcccgtggc tgattcgcgt gactttaaat
     1201 ttatcgcgca atcagctgcg ctcgaatttt cgttccaacg tgcgcgagga aacttacgtc
     1261 aaagaagcag acgaagcggg cagttttacg attgaccacg aaaccgagct tgaacgccgc
     1321 cagcaggcgg aagaagttca ccgcgcgttg aataaaatca aagagccgct caggagttgt
     1381 ctggttttga aacaacaggg cttaagctac aaggaaatcg ccgagagctt atcgctgaac
     1441 gaaaccagca tcggacaatt cgtggcgcgg gcgagaaaag agtttatgag attttacgga
     1501 aaaatcggta aggaggaagc gtgatggaca gatgttttga cgaaggcata gttcaagcct
     1561 ttttagacgg cgaactggct tccgatttat cggaaaatgt ggcttgccac gtggcgctgt
     1621 gcgaaaaatg cgcgatgatg ctcgccgcgg cggaagaaga atcgtcgttt gcttttgcgg
     1681 ctctcgacgc ggaatttaac gcgctcgtgc cgaccgaaag aatccggacg cgtctgtttg
     1741 aaacgatcgc tacggagcga aaaccgttct ggcagaaaat tttcggcaaa ggttttcgct
     1801 ggacaaatcc gtcagtcgcg gcgtttgcga gtttgatttt agtggtcggg ctttttgcga
     1861 cgctctggat tgcgcgcgaa aattcgatga aggaaggtca aatcgccggc atcgaaaaac
     1921 cgcaatcgac ggtcgccgcg ccgctctctc cgacaaaaag cgtcgattcg acgcccgccg
     1981 tcaatcctcc cgttcctttt gaatatgttg agccggtcgc gccgcagatt cgtcaggcgt
     2041 cttacaaacc gaaaaacgaa aacaggttcg aagcgcaaaa agcagtttat aaaacgggta
     2101 ataatatcgt cagaccgccg attgaccggg cgcccgcgga aacgaattac aacgcgattt
     2161 ccggcgagga aagctatctg aaaaccatcg ccacgctcca gcaatcggtc gataaccgga
     2221 aggacgaatt gatgaagcct tcgatgcgcg tggcatttga aaaagatatg gcggtcgtgg
     2281 acgacgcgat taagaaaatg cagcgcgaag ttcgcaaaaa cccgaaaaat gaagccgcca
     2341 gagaagttct gcgctcttcg tatcaaaaca aaatagattt gttaaattca gtcacggaaa
     2401 aaagcgaatt aatggcgagc cttaattcag gaaaataaac cagagagtga aaaaggaaaa
     2461 aggaaaattt tttatccgcg gctatgaggc gtcgcaaaag gcaattcttt tgtcttttgc
     2521 gtttttcctt cttttctccg cttcggaagt cttctctcaa atcgataaaa acgagccgaa
     2581 agtaattgtc gtcgttccgc cggcggtgca gccgacgcct ccgccgcgac cgtcgattcc
     2641 gagaaagcgg agagccgaca acgaaagcac ggcggcggct gaaaaatcga ttcgaaccga
     2701 agccaaagtc aacgtttcgc tctgcgtttc cgaaggcaat gtccgcatca acggctggga
     2761 gcgcaacgaa attcgcgctt tcgtcgacgg cggcagtaaa gtcggcttta aagtgatgca
     2821 gaaaaagcag aatccggtct gggtgatggt tctgggcttt gaccctctga cggataaaga
     2881 gccgggtttg gacgaatgcc tttcgggcgc gaatatcgaa ctggatgtgc cgcgcggcgc
     2941 gattatcaat atgaaaagcc gcgccagcca aatctcggtc gaatcgatcg ccagagtaaa
     3001 aatcgaaaac atcggcggcg atatttcgct caacaatatc gcgggcggca tcgacgccgg
     3061 gacttaccag ggcgacgtaa tggtcgaaaa atcggcgggc gcgatgtcgc tttttgcgac
     3121 gaccggaaat atcgtcgttt tcgacgtcga gccgagcgag gtcggcgaca gctttcgggc
     3181 gaaaacccgc aacggcacgg tgacgctgca atcggtcggg cattcgctcg tcgaagccaa
     3241 ctcgaccagc ggctcgattc gcttcaacgg cgaatttacc ggcggcgggc aatacacttt
     3301 cgtcaccaac aacggcacga ttctgctttc cgtcccggcg gaatcttcct gccgcatcaa
     3361 cgccaattac gggctcggcg cgtttcaatc cgacatcgcg ctgaaagacg tgcagaaaac
     3421 ttccggcgcg cgggtgcaga aactgacggc gctgctcggc gcgggcgacg ccaatctgac
     3481 tctcaccacc tacagcggcg cgattcgcat caagaaaaaa taatttaacg gtaaaaaata
     3541 aaaaggcgaa attaagattt tacttttagt ttcgcctttt tattttttac cgttgcgcga
     3601 tttgcggaag atgaataaag gtaaaattac aaataatttg ccttttgcct ttgaccgtca
     3661 aattatttgt aaaagaaaat caaaaaactt gtcacaaact ttttcggcgc ggtgttcttc
     3721 aattagacac gggaagtttc cttccaaaat tcagcgagga gaattaataa ataatatgtc
     3781 ttgggttata tctttagttt tagccggttt gatgattacg ccggacagca atctttccat
     3841 taatgccgat aataattttg tcaacgccaa cgcgccgacc gtcgtcagac tcgacgagac
     3901 cgagcgcttc gagcagactt atcccttaaa cgccggcggc agagtcagcg tctccaacgt
     3961 caacggctcg attatcgtcg aagcctggga cagaaacgag gtcagactgg aagcggtcaa
     4021 aaccgccgac acgaaagagc atctggcgga agtgcaaatc aaaatcgacg cgcgtcagga
     4081 ttcgttcagc gtcgaaaccg attacggttc gtggaagcgc aacaacgacg gcgtctggaa
     4141 aaataaaaat tacggcaagc tcgaagtgca gtatcgccta caggttccgc gcggcgcggt
     4201 tttggacgaa gtcgagacgg tgaacggctc ggtaacgatt tccaatatga ccaactcgac
     4261 caaagcttcc gccgtcaacg gaaacgtcaa agcgactaac ctgcgcggca cggcgcggat
     4321 ttcgaccgtc aacggcacga ccgaagccga tttcgattcc ttagccggca ccggtcaaat
     4381 tacgctcgat acggtcaacg gaaaagttct tttgacgata ccttcggacg cgaacgcaac
     4441 cgtcagagcc gatactttaa acggtcagat ttacaacgat ttcggtctgc cggtgcgcaa
     4501 gggcgaatat gtcggacgtg atttatacgg caaaatcggc agcggcgcgc tgaacatcaa
     4561 gttgagcagc gtcaacggcg agctgtcgat tcggcgcaaa caggacggca aaagcgtcag
     4621 cccggcgacg aatcttttgc cgcaaaaaaa taaagccg
//
LOCUS       DASWNE010001042         4708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_139780, whole genome shotgun sequence.
ACCESSION   DASWNE010001042 DASWNE010000000
VERSION     DASWNE010001042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139780"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..336
                     /locus_tag="VGB68_19890"
     CDS             46..336
                     /locus_tag="VGB68_19890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051570.1"
                     /translation="MRPFDTFGPLPNDDVRGRLDNYFVALRNDPTSTGYIVIDGPERA
                     ASRLETLVRNHIRFRRFDASRITIVRGDTTTPEVFTRLYVIPAGRTSADIER"
     gene            723..2861
                     /locus_tag="VGB68_19895"
     CDS             723..2861
                     /locus_tag="VGB68_19895"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEY0051571.1"
                     /translation="MVFSACLFANTAAALALGELNNEAKIFKGWQIIGPSGGDVRAVA
                     IDPKDKNRLYISTLDGQVHTSGDAGKTWQFLVNFNRPLLILDNLTVDTRDSNIIYTSG
                     HRHKEPGGFFKSTDGGATWKESKELKSEAIHAMVQSSSNPDIILVGGINGIWISNNSG
                     DDWKKIESATLPLNIDSLAIDPRDPKLIYAGTTWRAYKTTDGGKNWRLIKNGMLDDSD
                     VFAIEIDQKNPDHVIASACSGIYESFNKGEKWAKIQGIPSQSRRTRDIKINPANSGAY
                     YAATTEGFWMSNASGKAWMMTTSRELEINSIAVHGEESNRIFIATNNYGVMVSNDGGK
                     NFEPTNGNFTSRFTLSVVPDSERPNRFYAATHNTATGGGFFFISDDAGVTWQPAMKNL
                     VANRISPYTLLQDRVDPNKIYMGTNNGVFRSIDRGLSWTQLTPPKPKAVPKKRAATTK
                     RKTAVKTKAKPAPVETAPVTPPVDTTGKVAALTEQINIFVHTEDGKNGIIAGTDTGLY
                     RTYDINAGWEKIYFGEGITEKVFSIYTSPAHPGIIWVGTATSGLLLSRDDGETWQKVA
                     GVPVDAPISAIVVDEKRPERIYVGTAQTLYLSRDGGKNWMRRGGNLPLGNYTSILVNP
                     RNTDEILVASALEANGGIYQSADAGATWKRVDTKEMNMASRRYWTMTFDPSDPERILV
                     GTHSSGIYRLERTPAAASNNTRPRISSTGN"
     gene            2959..4626
                     /gene="bshC"
                     /locus_tag="VGB68_19900"
     CDS             2959..4626
                     /gene="bshC"
                     /locus_tag="VGB68_19900"
                     /inference="COORDINATES: protein motif:HMM:NF021586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HEY0051572.1"
                     /translation="MNHESVGLLQKASFKIEPLSFAEITGQTRLFLDFQADSANISRF
                     YPEKQTPLANFAPKVLENYKIDRDALCDVLEETNRGFGGGQKTLENINLLRQKDVVAV
                     VTGQQAGLFSGALYTIYKALSAVRLAEDLQKQNIRAVPVFWIAEEDHDFDEVKKTFAI
                     DKGGKLFELENSPEARVENQPVGAVRLDATIEETIGELQQNLLKTEFSRELKEVLSEA
                     YRAGETYSTSFARFIAKLFAAKGLIIFSPLNEKLKRLSAPIFVEAVKKNGEISESLLS
                     RTERLDAENYHAQVLVEEDFFPFFLFDENGKRRALKKTKENNFKAKDARREFSLDELI
                     EIAESSPQNLSPNALMRPVVQDYLLPTICYFGGAAEIAYFAQNAEIYRILNRPATPVR
                     HRASFTVIEAKHARTLDRYKLKFKDLFAGQERIFAEIVEKFLADTAAREFAEVEEIIN
                     TQLNRLDRSLLASEPTLSPNLATRRRKIMYHIGALRKKFYRAEIFKDQTARRRVETLF
                     AALLPHKALQERTLNAVYFLNLYGENFIEWLYEATGADEKGHQILYL"
BASE COUNT         1238 a         1197 c         1201 g         1072 t
ORIGIN      
        1 ccgagaaacc tgtcagaaac aggttcagtt ggtccgtcac cgagagtgcg tccgtttgat
       61 accttcggac cgctgccgaa cgacgatgtc agaggcagac tcgacaacta cttcgtcgcg
      121 ctcagaaatg acccgacgtc gaccggttac atcgtgattg acggtcccga aagagcggct
      181 tcgagactcg aaacgcttgt ccgcaaccac atcagattcc gtagattcga cgcgagccgc
      241 atcaccatcg tcagaggtga tacgaccacg cccgaagtct ttacgagact ctatgtgatt
      301 ccagcgggca gaacgtcagc cgatatcgaa cgataaatta gctttcactg ctgcttcggc
      361 ggcagtgaaa catttcatca aaaacgggta atgcttcttt tttgaaagca ttacccgttt
      421 ttctttaagt atttctttaa gtttttgcgc aaaactcttg ccaagtgtta aacagttgtg
      481 gataataaac agcagagaag tttcgtccgc gctcgacggc ttccgttcac aattcgcaaa
      541 attcatgcat ttgataagtc taaggttaaa ggcaacaacc tttcgggtta tatacatctg
      601 agcaatgagt tgtttatttt tttaactggc gggcttccac gtttagttca aaagtttcaa
      661 tagaaaagtt ttaatctatg aaaacagtaa aagatagtat tgcgttgaga ttactaacca
      721 tattggtatt ttcagcttgt ctgttcgcta acactgcggc tgcgctcgcc ctgggcgagt
      781 taaataacga agccaaaata tttaagggct ggcagattat cggtccgagc ggcggcgacg
      841 tgcgcgccgt ggcgattgac ccgaaagaca aaaatcgtct gtatatcagc actttagacg
      901 ggcaggtgca tacctccggc gatgccggta aaacgtggca gtttctggtt aattttaatc
      961 gcccgctgct gattctcgat aatttaaccg tcgacacgcg tgattcgaac atcatctaca
     1021 cttcggggca tcgacacaaa gagccgggcg gattttttaa atcgaccgac ggcggcgcga
     1081 cctggaaaga atcgaaagag ctgaaaagcg aggcgattca cgcgatggtg cagtcttcct
     1141 cgaatccgga cattattctg gtcggcggca tcaacggcat ctggatttcc aataattccg
     1201 gcgacgactg gaaaaaaatc gaatctgcaa cgctgcccct gaacatcgat tcgctggcga
     1261 tagacccgcg cgacccgaaa ctgatatatg ccggaacgac gtggcgcgcc tataaaacga
     1321 ccgacggcgg aaaaaactgg cggctcatca aaaacgggat gctcgacgat tccgacgttt
     1381 tcgccatcga gattgaccag aaaaatcccg accacgttat cgcttcggct tgcagcggca
     1441 tttacgaatc ttttaataaa ggcgaaaaat gggcgaagat tcagggaatc ccttcgcaat
     1501 cgcgccgcac gcgcgacatc aagattaatc cggcgaattc cggcgcttat tacgccgcga
     1561 cgaccgaagg cttctggatg tcgaacgcct ccggcaaagc ctggatgatg acgacttcgc
     1621 gcgagctgga aatcaattcg atcgccgttc acggcgagga atcgaatcga atctttatcg
     1681 ccacaaataa ttacggcgtg atggtttcaa acgacggcgg aaaaaatttc gagccgacca
     1741 acggcaattt taccagtcgt tttacgctct cggtcgtgcc cgacagcgag cgcccgaacc
     1801 gtttctatgc ggcaactcat aacacggcga ccggcggcgg atttttcttt atcagcgacg
     1861 acgcaggcgt gacgtggcag cccgccatga aaaatctggt cgccaatcgc atttcgccgt
     1921 atacgttgct gcaagaccgc gtcgacccga acaagattta tatgggcact aacaacggcg
     1981 tctttcgctc gattgaccgg gggctttcgt ggacgcagtt gacgccgccg aaaccaaagg
     2041 cagtgccgaa aaagcgcgcc gcgacgacaa agcgcaaaac cgcggttaaa accaaagcga
     2101 aacccgcacc cgtcgaaacc gcgccggtta cgccgcccgt cgatacgacc ggcaaagttg
     2161 cggctctgac cgagcagatt aatattttcg ttcataccga ggacggtaaa aacggaatta
     2221 tcgcgggcac tgacaccggc ttgtatcgca cttatgatat taacgccggt tgggaaaaaa
     2281 tctacttcgg cgaaggcatc acggaaaaag ttttctcaat ttatacttcg cccgcgcacc
     2341 ctggaattat ctgggtaggg acggcgactt ccggcttatt gctttcgcgc gacgacggcg
     2401 agacttggca gaaggtcgcg ggcgtgcccg tcgacgcgcc gatcagcgcc attgtggtag
     2461 acgagaaacg ccccgaaaga atttacgtcg gcacggcgca gacgctgtat ctgtcgcgcg
     2521 atggcggaaa aaactggatg cggcgcggcg gaaatctgcc gctcggaaat tatacgagca
     2581 ttctcgtcaa cccgcgcaac acggacgaaa ttctcgtcgc cagcgcgctg gaagccaacg
     2641 gcggaattta ccagtcggct gacgcgggcg ctacctggaa gcgggtcgat acgaaagaga
     2701 tgaatatggc gagccggcgt tactggacga tgactttcga cccgtccgac ccggaacgca
     2761 ttcttgtcgg aacgcattcg tcgggaattt accgtctgga acgaactccg gcggcggctt
     2821 ccaacaacac gcgcccgcgc atttcgtcga cgggcaatta attctgtggg taaatgaaaa
     2881 atgaaggacg agttccgctt cggcagaact cgtccttttc gtttgtaact tggcggaatt
     2941 gaatcttata attgcctgat gaatcacgaa tcggtcggtc ttttgcagaa agcttctttc
     3001 aaaatcgagc cactttcctt tgccgaaatt accggtcaga cgcgtttgtt tctggatttt
     3061 caggcggatt ctgcaaatat ttcccgtttt tatcccgaaa aacaaactcc gttggcgaat
     3121 ttcgcgccga aagttctgga aaattataaa attgaccgcg acgcgctgtg cgacgttctg
     3181 gaagaaacaa atcgcggttt cggcggcggg caaaaaactc tggaaaatat aaatctgctg
     3241 cggcaaaaag atgtcgtcgc cgtcgtgacc ggacagcaag ccggactttt ttccggcgcg
     3301 ctctacacga tttacaaagc gctttccgcc gtgcggcttg ccgaagattt acaaaaacaa
     3361 aacatccgag ccgttcccgt tttctggatt gccgaagaag accacgattt cgacgaggtt
     3421 aaaaagactt tcgcgattga taaaggcgga aaattattcg agctggaaaa ctcgcccgaa
     3481 gcccgcgtcg aaaatcagcc ggtcggtgcg gttcgattag acgcgacgat tgaagagaca
     3541 atcggagagc ttcaacaaaa tctgctgaaa acggaattca gccgcgagct gaaagaagtt
     3601 ttaagcgaag cttatcgcgc gggcgaaact tattcgacga gttttgcccg ctttatcgcc
     3661 aaacttttcg cggcgaaagg cttgataatt ttttcgcctt tgaacgaaaa gctaaaaaga
     3721 ctttccgcgc cgatttttgt cgaagccgtg aaaaaaaacg gcgagattag cgagagcctg
     3781 ctttcgcgaa ccgaacggct cgatgcggaa aattatcacg ctcaggtttt ggtcgaggaa
     3841 gatttctttc cgtttttcct gtttgacgaa aacgggaaac gtcgggcttt gaaaaaaaca
     3901 aaggaaaata atttcaaagc gaaagatgcg cgccgggaat tttcgctcga cgaattaatc
     3961 gaaatcgccg aaagctcgcc gcaaaattta agcccgaacg cgctgatgcg tcccgtagtg
     4021 caggattatc ttttgccgac catctgttat ttcggcggcg cggcggaaat cgcttatttt
     4081 gcgcaaaatg cggagattta tcgaattttg aatcgaccgg cgacgcccgt tcgtcatcgc
     4141 gccagcttta ccgttatcga agcgaaacac gcgcgcacgc tcgaccgcta taaactgaaa
     4201 ttcaaagatt tatttgccgg gcaggaaagg atttttgccg aaatcgtcga aaaattttta
     4261 gccgacaccg cggcgcgcga attcgccgaa gtcgaggaaa tcatcaacac tcagctaaat
     4321 cgcctggacc gcagcctgct ggcgagcgag ccgactttat ctccgaatct ggcgacgcgc
     4381 cgccgcaaga ttatgtatca catcggcgcg cttcgtaaga aattttaccg cgccgaaatt
     4441 ttcaaagacc agacggcgcg ccgccgcgtc gaaacgcttt tcgccgcgct tctgccgcat
     4501 aaagccttac aggaacgcac gctgaatgcg gtttattttc tgaatttata cggcgaaaat
     4561 tttatcgaat ggctttatga agcaaccggc gcggacgaaa aaggacatca gattttatat
     4621 ttgtaatgaa taaaataaaa gttttacccg acaatctggc aaatcaaatc gcggcgggcg
     4681 aagtcgtcga acgtcccgcc tcggtcgt
//
LOCUS       DASWNE010001043         5643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_528923, whole genome shotgun sequence.
ACCESSION   DASWNE010001043 DASWNE010000000
VERSION     DASWNE010001043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5643
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528923"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2735
                     /locus_tag="VGB68_19905"
     CDS             <1..2735
                     /locus_tag="VGB68_19905"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY0051573.1"
                     /translation="FRADFRAYFKDGYRDYNTANGANGWNFGASGFGSYASGAGNARE
                     LAIPWSTITGGGRPASFLFLAYLSSSGGYIYGQVPQGNAEGNSPGNYNRYYAVVNTEN
                     GTSTKPFSLDQSVSTNDIQYLGLKHDTFDSYYRSPFGAATTNSSVNLKFRTDLLDVSG
                     VFLKVYRYNPADGTTDPPVEYPMSFLENRTEGATTYDVYSVNYTTPATPSIIYYKFRV
                     VDGGSQAFYSDAYGDDHDNLGQGGDGSASDGEPFPAFQITVYPASFTTPAWIHNAAVY
                     HIFPDRFRNGDITNDWCRGNPIGCPSLYGAPPESNIIHTIWNEAMVDPRATGNNNAYG
                     SQFYGGDLKGVQDKLDYIKNMGFDTIYLNPIFKARSNHRYDTDDFLGIAPELGGDAAF
                     ASLVAAAEARGMKIILDGVWNHMSQDSEYFDYYQRSPVVGACESTSSAYRSWFNFFNT
                     DAPCDFTDYEGWFGFGGLPVLNENDAVKNFFYRTPTTNVTQYWYDRGANGWRFDVATD
                     ISHQWWNEYRGYAKTYKPDGVLIGEIFPDASQYLAGDQLDGSMNYRFRKNVLGYARGF
                     DWEDNDNNGGNKILGLTPSQFNRSMLAIREDYPLPAQRSMLNLLDSHDTNRALYTLTV
                     SGDSGLTQAKERLKLAAIFQFTYIGAPMVYYGDEAGINSPSLGNSGGGLPEDDPYNRA
                     PYPWADESGNQNVYGPADNALINFYTTLGAFRRNHPAVRTGSFEALLMGDITASATDN
                     NTFAFARAQESDKVIVVMNNGSTANTATIPVGAYYADGTILYDNLGNSSVAAGKNSLA
                     SHTVSGGTITVTIPARSGAIFTNLAPTAASVSVSGTVLTATARPISGATVTITDTGGE
                     NRTAVTNPFGHYQFEGIPAGETYVLGVRHKRYQFPSRAITVNEDLSEVDISAMP"
     gene            complement(2828..3439)
                     /locus_tag="VGB68_19910"
     CDS             complement(2828..3439)
                     /locus_tag="VGB68_19910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEY0051574.1"
                     /translation="MANKFYPANDAEFAVWLAILLNKASLNKTTLQVSEERLTALNTK
                     LSAFNENLALKRQKREESIAQTTLVSNLRKDLNKEISLLNNGFKAIDGLPPNLLEELG
                     LSVNEGSSGTTPPSAPSDLVVTGTSDGVNSLKWNRSGNRQGTTFIIEAKIGSASNWTI
                     IDALTGSTYKHKNQTPGVTAQYRVRAKRGESESGFSNTAVVYG"
     gene            complement(3497..3787)
                     /locus_tag="VGB68_19915"
     CDS             complement(3497..3787)
                     /locus_tag="VGB68_19915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051575.1"
                     /translation="MNDREMGFFVRENVFRLGEIKFRLGKKLLKPRKKVLELEEKLPK
                     PREKLLKLKKKVLKLREKLLKLREKLFFSEGKFIFFSEKLLFDKKKAVGVGF"
     gene            complement(3917..5107)
                     /locus_tag="VGB68_19920"
     CDS             complement(3917..5107)
                     /locus_tag="VGB68_19920"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0051576.1"
                     /translation="MPEEIFWETCALINPGWQNYLADEDSRDRIYRKLAQYKLFDRDG
                     KAETFTIHRLVKLFFEDKLKDERRTIEKPSAEILAEHFEHFDFTNKEKVERYLSHVGV
                     FLEYLEENKSETKFNLKLENKSTVRLCNSYARHFELYGQYKKAEIYYELFKDICEKCE
                     DIDQTWTATSYNNLALLYFSQGRYEEAEPLYQKALEIREKLLGENHPDTATSYNNLAG
                     LYKLQVRYEEAEPLYQKALEIREKLLDENHPSTATSYNNLALLYDSQGRYEEAEPLYK
                     KALEIKERVLNENHPSMATSYNNLAGFYLERGNLELAKQYQQKSLSARLTIFGENHPD
                     VAQSYWGLGIFCVKEEKYTEALDYFYKAREIFLRFLPEEHPDLINLQGFIDKYENYLA
                     NEEK"
     gene            5168..5389
                     /locus_tag="VGB68_19925"
     CDS             5168..5389
                     /locus_tag="VGB68_19925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051577.1"
                     /translation="MFFVRFVFRVANRIKGEVEGAVNVWMLVGVDAFFVFENFSPRIL
                     QAVEVFGKGFTVFVDVISGNEYCQRVKAE"
BASE COUNT         1345 a         1464 c         1335 g         1499 t
ORIGIN      
        1 cgtttcgcgc cgatttccgc gcttatttta aagacggcta ccgcgattac aacacggcaa
       61 acggcgcgaa cggctggaat ttcggcgcgt ccggttttgg cagctacgct tcgggcgcgg
      121 gcaatgcgcg cgagctcgcg attccctggt cgacgataac gggcggcggg cgacccgctt
      181 catttctttt tctggcttac ttgagttcga gcggcggata catttacggg caggttccgc
      241 agggcaacgc cgagggcaac tcgccgggaa attacaatcg ttattacgcc gtcgttaaca
      301 cggaaaacgg gacgagcaca aaacctttct cgctcgacca gagcgtcagc accaacgaca
      361 ttcaatatct cggcttgaaa cacgacactt tcgacagcta ttaccgctcg ccgtttggcg
      421 cggcgacgac gaattcgagc gtcaatctca aatttcgcac ggatttatta gatgtcagcg
      481 gcgttttcct gaaagtttac cgatacaatc cggcagacgg cacgaccgac ccgccggtcg
      541 aatacccgat gtcgtttctg gaaaatcgca cggaaggcgc gacgacatac gacgtttaca
      601 gcgtcaatta cacgacgccc gcgacgcctt cgattattta ttacaaattc cgcgtcgtcg
      661 acggcgggtc gcaggctttc tacagcgacg cgtacggcga cgaccacgac aatctcggtc
      721 agggcggcga cggttcggca tcggacggcg agccttttcc ggcgtttcag ataacggttt
      781 atcccgcaag ttttacgact ccggcttgga tacacaatgc ggcggtttat catattttcc
      841 cggaccgttt tcgcaacggc gacattacca acgactggtg tcgcggaaat cccatcggct
      901 gcccgtcgct ctacggcgcg ccgcccgaat cgaacatcat tcacacaatc tggaacgaag
      961 cgatggttga cccgcgcgcg accggaaaca acaacgctta cggcagccag ttttacggcg
     1021 gcgatttgaa aggcgtgcag gacaagctcg attacattaa aaatatgggt tttgacacga
     1081 tttacctgaa cccgattttt aaagcgcgtt ccaatcaccg ctacgacacg gacgattttc
     1141 tcggcatcgc gccggagctc ggcggcgacg cggctttcgc gtccttagtg gcggcggcag
     1201 aggctcgcgg aatgaaaatc attctcgacg gcgtctggaa tcatatgtcg caggacagcg
     1261 aatatttcga ttattaccag cgctcgcccg tcgtcggcgc gtgcgaaagc acaagctcgg
     1321 cttatcgaag ctggttcaat ttcttcaaca cggacgcgcc gtgcgatttc accgactacg
     1381 aaggctggtt cggcttcggc ggtctgcccg ttttaaacga aaacgacgcg gtcaagaact
     1441 ttttctaccg cacgccgacg accaacgtca cgcaatactg gtatgaccgc ggagcaaacg
     1501 gctggcgctt cgacgtggcg accgacatct cgcatcaatg gtggaacgaa tatcgcggct
     1561 acgccaaaac ctacaagccc gacggcgttc tcatcggaga aattttcccc gacgcgagcc
     1621 aatacctggc gggcgaccag ctggacggct cgatgaatta tcgtttccgc aaaaacgttc
     1681 tcggctacgc gcgcggcttc gactgggaag acaacgacaa caacggcggc aataaaatcc
     1741 tgggtttgac gccttcgcag tttaaccgct cgatgctggc gattcgcgaa gattacccgc
     1801 ttccggcgca gcgctcgatg ctcaatctgc tcgactcgca cgacacgaac cgcgcgcttt
     1861 acactttaac cgtctccggc gacagcggct tgacgcaggc gaaagagcgt ctgaagctcg
     1921 cggcgatttt ccagttcaca tatatcggcg cgccgatggt ttactacggc gacgaagccg
     1981 gaataaattc gccttcgctc ggcaatagcg gcggcggctt gcccgaagac gacccgtata
     2041 accgcgcgcc gtacccgtgg gcggacgaga gcggaaatca aaacgtatac ggtccggcag
     2101 acaacgcgtt aatcaatttc tacacgacgc tcggcgcttt ccggcgcaat catccggctg
     2161 tgcgaaccgg ctcgtttgaa gctcttttaa tgggcgacat cacggcgagc gcgaccgaca
     2221 acaacacttt cgccttcgcc cgcgcgcagg aaagcgacaa ggtcattgtc gtaatgaaca
     2281 acggctcgac cgccaacacg gcgacgattc cggtcggcgc ttactacgcg gacggaacga
     2341 ttttatatga caatctggga aacagctcgg tcgccgcggg caaaaattcg ctggcgagtc
     2401 atactgtttc aggcggcacg attacggtca cgattcccgc ccgcagcggc gcgattttca
     2461 ccaatctcgc gccgaccgcc gcctcggttt ccgtcagcgg aacagtcctg accgccaccg
     2521 ctcgccccat ttccggcgcg acggtcacaa tcacggacac cggcggagaa aaccgcaccg
     2581 ccgtcaccaa tcctttcggt cattatcaat tcgaaggaat tccggcgggc gaaacctatg
     2641 ttctcggcgt ccggcacaag cgttatcaat tccccagccg ggcaatcacc gtcaacgagg
     2701 atttgagcga ggtcgacatc tcggcgatgc cgtaatgaca aaacgatgaa ggcggaacgc
     2761 ggaacgatga acgataaatt gcaattttgc ttatcgaatt catcattcat cgttccgcct
     2821 tcatcgttta tccatagacg acggcagtat ttgaaaagcc gctttccgat tcgccgcgct
     2881 tcgccctgac gcgatactgc gccgtcacgc cgggcgtctg atttttgtgt ttataagtcg
     2941 agccggtcaa agcgtcgata atcgtccagt tactcgcgct tccgattttc gcttcgataa
     3001 taaacgtcgt gccctgacga ttgccgcttc tattccattt cagcgaattc acgccgtccg
     3061 aagttcccgt cacgaccaaa tccgacggcg cggacggcgg cgtagtgccg gagctgcctt
     3121 cgttgacgct caaaccgagt tcttccagca gattcggcgg cagaccgtca atcgctttaa
     3181 acccgttatt cagcaaactg atttctttgt tcaaatcctt tcgcagattg ctgaccagcg
     3241 tcgtctgcgc gatagattct tccctctttt gccgtttcag cgccagattt tcattaaacg
     3301 cgcttaattt tgtatttaac gccgtcagtc tctcttcgct tacttgaagt gttgttttgt
     3361 ttaaagacgc cttgttcaga agaatcgcca gccagacggc aaattcggca tcattggcag
     3421 gataaaattt gttagccatc gtgtatatct cctttccgaa catcagaatc gagactttcg
     3481 ggctagtcgg tcaacctcag aatccgactc cgacagcttt tttcttatca aacaaaagtt
     3541 tttcgctaaa gaaaataaat tttccctcgg agaaaaaaag tttttcccta agtttcaaga
     3601 gtttttctct aagtttcaga acttttttct tgagttttaa aagtttttcc ctaggttttg
     3661 gaagtttttc ctcaagctcc aaaacttttt tcctgggttt taaaagtttt ttcccaagcc
     3721 gaaacttaat ttccccaagc cggaaaacat tttccctcac gaaaaacccc atttccctgt
     3781 cattcaaaag tttcttcctc acgaaaactc gccggaaacc aactataaac tttcaaataa
     3841 cgctgcattc gtccaacaaa caaatatata aaataaaacc cgcttttcgt caacaaaaat
     3901 tttcgctcgc cgcaaattat ttttcctcat tcgccaaata attttcatat ttatcaataa
     3961 aaccttgtaa gtttattaaa tcaggatgtt cttccggtaa aaatctcaag aaaatttcgc
     4021 gtgccttgta aaaataatct aaagcttccg tgtatttttc ttcctttacg caaaatatgc
     4081 cgagccccca ataactttga gccacatcag gatgattttc accgaagata gtcaaacgcg
     4141 cagacagcga tttctgctga tattgtttgg caagttcaag atttcctctt tcaagataaa
     4201 atccggctaa attattgtag cttgttgcca tagaagggtg attttcattt aatactcttt
     4261 ctttaatttc taatgctttc ttgtataaag gctcggcttc ttcatatctg ccttgtgaat
     4321 cataaagcaa ggctaaatta ttatagcttg tggctgtact aggatgattt tcatctaata
     4381 gtttttcacg aatttctaac gctttttgat ataaaggttc ggcttcttca tatctgactt
     4441 gtaatttata aagtccagct aaattattgt agctcgttgc tgtatcagga tgattttcac
     4501 ctaatagttt ttcacgaatt tctaacgcct tttgatataa aggctcggct tcttcatatc
     4561 tgccttgtga aaaataaagc aatgctaaat tattgtagct tgttgccgtc caagtttggt
     4621 cgatgtcctc acatttttcg caaatatctt taaatagctc gtaataaatt tctgccttct
     4681 tatactgtcc atacagttca aaatgtcgcg cgtaactatt gcataaacgt acagttgatt
     4741 tgttttctaa tttgagattg aattttgttt cggatttgtt ttcttcgaga tattctaaaa
     4801 acacgccgac gtgcgataaa tatcgctcga ctttttcctt gttggtaaaa tcaaaatgtt
     4861 caaaatgttc ggctaaaatt tccgctgacg gtttttcgat tgttcggcgc tcgtctttta
     4921 atttatcttc gaaaaataat ttgacgaggc ggtgaatcgt gaaggtttcg gctttgccgt
     4981 cgcggtcgaa aagtttgtat tgggcgagtt ttcgatagat tcggtcgcgg ctgtcttcgt
     5041 cggcgagata gttttgccag ccgggattta ttaaggcaca ggtttcccag aagatttctt
     5101 cgggcatatt gtcgggcgcg aggacggacg cgattttcat acattcgcgg gcgattgcga
     5161 ttcgggtttg ttcttcgtcc gatttgtttt ccgcgtcgca aatcgcatta aaggcgaggt
     5221 cgaaggcgct gtaaatgtct ggatgctggt aggcgttgac gcctttttcg tctttgagaa
     5281 tttttccccg cgaattttgc aggcggtcga ggtatttggc aaaggtttta cggttttcgt
     5341 tgacgtaatt tccggcaatg aatattgcca gcgggtgaaa gccgaataaa cggacgattt
     5401 cgcggattgc ggcttgttct tcggcaggca gacggtcgag atttttcgcg tcggttaagg
     5461 cggcaacgcg ataaagcaaa agcatcgcat cggattccgg taaattgccg atttcgacct
     5521 gtcgtccgag acgataaacg cgttcaaaat tcgatgtcag caggacgttt aacttcgcgt
     5581 gacgcggcag aaatttttgc aggcgctcgg tttcgtcaac gttgtcgaat atcaaaagca
     5641 ggc
//
LOCUS       DASWNE010001044         1714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_705136, whole genome shotgun sequence.
ACCESSION   DASWNE010001044 DASWNE010000000
VERSION     DASWNE010001044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705136"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="VGB68_19930"
     CDS             <1..780
                     /locus_tag="VGB68_19930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051578.1"
                     /translation="VQRALAKVPENRYQSANDLADALNRATVFAGRASPDAVAAHALV
                     ASQPQNNLWKTAFIVLAGIAVLSGFFIYMTQVKQTNPATSLQTDANGSPVQPVNPATG
                     MTEQGLSNMGAYNPLQDGNINAMPQDAMPGGGGAGYGNPWERSTNPPPGAPPQQYVPP
                     TGQYYDGNINPGSPFMAPDGNVYMAVPKNTNTNVNTPPKPRTSPTQAANTQPAPSNTK
                     PPANTTTPTEPKTTPAPRPTAKPAEQPKTDKPTSSGKVQDS"
     gene            complement(994..>1714)
                     /locus_tag="VGB68_19935"
     CDS             complement(994..>1714)
                     /locus_tag="VGB68_19935"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEY0051579.1"
                     /translation="HRRVRRDLDIVCVDAANPFGNGKLLPGGILREPLSNLKRADAVV
                     ITRANLIGAQRIADLKLRISEINPRCKIFVSKNKTSNLINLDEFNASVQSAKDAKSAP
                     GGLKREENRSQVNTGEHGFENEDQSSTGYEQRATNNYLAFCALGNPESFFGQLRRENF
                     ELIFTKAFRDHYRYTQKDVDELEREAAARGAQSFLTTAKDAVKLKDLQFNLPCRVVES
                     ELIFDDEEGFRKLIASAAKKN"
BASE COUNT          413 a          479 c          441 g          381 t
ORIGIN      
        1 gtccagcgcg cgctggcgaa agtccctgaa aaccgctatc aatcagccaa cgatttagcc
       61 gacgcgctga atcgcgccac cgtttttgcg gggcgagctt cgcccgacgc agttgcggcg
      121 cacgcgctcg ttgcgagtca accgcaaaat aatttatgga aaacggcatt tatcgtgctt
      181 gccggaatcg ccgtgctgag cggatttttt atttatatga cgcaggtcaa gcagacgaat
      241 ccggcgactt cgctgcaaac cgacgccaac ggctcgcccg tccagcccgt caatccggcg
      301 accggaatga ccgaacaggg tctttcaaat atgggcgcgt ataatcctct gcaggacgga
      361 aatataaatg cgatgccgca ggacgcgatg cccggcggag gcggcgcggg ctacggcaat
      421 ccgtgggaaa gaagcaccaa tccgccgccg ggagcgccgc cgcagcaata tgttccgccg
      481 accggacaat attatgacgg caacatcaat ccgggcagcc cgtttatggc gccggacggc
      541 aatgtttata tggctgtgcc gaaaaatacg aacacgaacg taaacacgcc gccgaaaccg
      601 agaacttcgc cgacgcaggc ggcaaatacg cagcccgcgc cgtcaaatac caaaccgcca
      661 gccaatacga cgacgccgac cgagccgaaa acaacgcccg cacctcgacc gaccgcgaaa
      721 ccggctgagc agcccaaaac cgataagccg acttcgagcg gaaaagtgca ggacagctag
      781 agattcagcc gccgaagaaa cgaaaaaaat cagccgccga agaacgctaa aaagcccgga
      841 aaaagaattt accggcgata agcgggcttc gcggcttgat ttacgggata aagaagaatc
      901 atctttcgta acccgctgtg aatttctttc tccgtgttcc gcggcggctg gtttcttagt
      961 tctttgttcg cgttctttgg tgtttgttgg cggctaattc tttttcgccg cgcttgcgat
     1021 caatttccga aagccttctt cgtcgtcgaa aatcaattcg ctttccacga cgcggcacgg
     1081 cagattaaat tgtaaatctt ttaatttcac cgcatctttg gcagtcgtca gaaaactctg
     1141 cgcgccgcgc gcggcggctt ccctttccag ctcgtcgacg tccttttgcg tgtagcgata
     1201 atggtcgcgg aaagctttgg taaaaatcaa ttcaaaattt tcgcgccgca gctgcccgaa
     1261 aaaactttcg ggattgccga gcgcgcaaaa ggctaaataa ttgtttgttg ctcgctgttc
     1321 atagcccgtc gaagattggt cttcgttttc aaatccgtgt tcgcccgtgt ttacctgtga
     1381 acgattttct tcgcgcttta aaccgcccgg tgctgacttt gcgtctttag cgctttgcac
     1441 ggaagcgtta aattcgtcca ggtttattaa attcgatgtt ttattttttg aaacgaaaat
     1501 tttacagcgc ggatttatct ctgaaatccg cagtttcaaa tccgcaattc gttgcgctcc
     1561 aatcaggttc gcgcgtgtaa taaccaccgc gtcggcgcgt ttcaaattcg acaaaggctc
     1621 gcgcaggatg ccgccgggca aaagtttgcc gttgccgaac ggattggcgg cgtcgacgca
     1681 aacaatatcc aaatcgcgcc gaacgcgccg gtgc
//
LOCUS       DASWNE010001045         2039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_705196, whole genome shotgun sequence.
ACCESSION   DASWNE010001045 DASWNE010000000
VERSION     DASWNE010001045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705196"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..929
                     /locus_tag="VGB68_19940"
     CDS             177..929
                     /locus_tag="VGB68_19940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051580.1"
                     /translation="MKVVLLALALLISAGCANHTAADSIGATAQTTRTVVNVNPQIDS
                     FPQGEKEKIDPEKRKALEAQNARFKVAPAEFAKVDFKNFKYPAARLKDGEFKHFQDKN
                     LGSGWFSLEDVFYVDLNEDEKSEAVVLLGSFGCGGSCDGGRTIIYFYSARKGKPRLLA
                     EIETGSRSGGCSLKSLAIKDRKIILEQFGNCDKNPTYEENRKYSCKFCVKDLTRSVYS
                     FKNGELVRETSETSETPETGIMNYTTEISIGH"
     gene            1071..1613
                     /locus_tag="VGB68_19945"
     CDS             1071..1613
                     /locus_tag="VGB68_19945"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HEY0051581.1"
                     /translation="MSEERIVTIFGGSRCKENSPEYEQAIEVGARLAEAGFTICTGGY
                     LGVMEAASRGAREKGGRVLGIVMNQFKAEPNRFLTDKVASEHFYERLQNLIIRSVGFI
                     ALKGGMGTITEISLVWNKLMTGVIDRRPMVLLGEGWKPVVECWQQHLVVSDEDVSLLD
                     FADTAERACEIILAKSPKTI"
     gene            1654..>2039
                     /locus_tag="VGB68_19950"
     CDS             1654..>2039
                     /locus_tag="VGB68_19950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051582.1"
                     /translation="MFKLSRRLSFVLAFCVFFFSNAFAQAKLLRFPDIHGEKVVFTYG
                     GDIWTAAANGGAAAATRLTAHAGVEVFAKFSPDGNWIAFTGQYDGDEQVYVMPAGGGE
                     PKQLTFYPAKGPLTPRWGYDNQVMGWT"
BASE COUNT          505 a          475 c          591 g          468 t
ORIGIN      
        1 tgccgcgcga atccttcgaa tggtatctgg atttgcgccg cttcgggtca gttccgcacg
       61 ccggtttcgg catgggcgtc gaacgctgca cggcgtggat gtgcggcatc gaacacgttc
      121 gggaaacgat tccgtttccg cgaatgcttt atcgcttgaa gccgtaagga ttaataatga
      181 aagttgtgtt attggctctg gcgttgttaa tttcggctgg ctgcgcaaat catactgccg
      241 ccgactcaat cggcgcaacc gcgcagacga cccggacggt cgtgaatgta aatccgcaaa
      301 tcgattcgtt tccgcagggc gaaaaggaaa agattgaccc ggaaaaacga aaggcattgg
      361 aagcgcagaa cgcgaggttc aaagttgctc ccgccgagtt tgcaaaggtt gattttaaga
      421 atttcaaata tccggcggcg cgtctgaaag acggagaatt caagcatttt caggataaaa
      481 atttaggcag cggctggttt tcattggaag atgtttttta cgttgatctg aatgaagatg
      541 aaaaaagcga agcggttgtt ctgctcggtt cgttcggctg cggcggctcg tgcgacggcg
      601 gacgaacgat tatctatttt tattcggcgc gcaaaggaaa gccgcgattg ctggctgaaa
      661 tcgagaccgg aagtcggtcg ggcggctgca gcttaaagtc gctggcgata aaagaccgaa
      721 aaattattct ggagcaattc ggcaactgcg acaaaaatcc gacctacgag gaaaaccgaa
      781 agtattcctg caagttttgc gtaaaggatt taacccgcag cgtttactcg tttaaaaacg
      841 gagagctggt cagagaaaca agcgaaacga gcgaaacgcc cgagaccggc attatgaact
      901 atactacgga aatcagcatc ggccattgat ttttttatga gaacacagag ccttttgaaa
      961 atgttgacgt ttataacata atgactcgga ttcttagttt agaagccggc acgcgggcga
     1021 cggcaattcg gcgacggcgg aaaggttttt gcgggaaaag ataaaataat atgagcgaag
     1081 aaagaattgt caccattttc ggcggctcgc gctgcaagga aaattctccc gaatacgaac
     1141 aggcgataga agtcggcgcg cggctcgccg aagccggttt taccatctgc acgggcggct
     1201 atctgggcgt tatggaagcc gcttcgcgcg gcgctcggga aaaaggcggg cgcgttctgg
     1261 gcatcgtgat gaaccagttt aaagccgagc cgaaccgctt tctgaccgac aaggtcgcat
     1321 ccgagcattt ttacgagcgc ctgcagaatc tgattattcg ctcggtcggc tttatcgcgc
     1381 tgaaaggcgg aatgggaaca atcacggaaa tctcgctggt ctggaacaag ctgatgaccg
     1441 gagttatcga ccgccgcccg atggtgcttt tgggcgaagg ctggaaaccg gtcgtcgagt
     1501 gctggcagca gcatctggtc gtcagcgacg aagacgtttc gctgcttgat ttcgccgata
     1561 ccgccgagcg agcgtgtgaa atcatcctgg cgaaatcgcc gaaaacgatt tgataagttt
     1621 tcgattcaaa aaattaaaaa tctcggagca aatatgttca aactttcgcg tcgtttgagt
     1681 tttgtgctgg ctttctgcgt ctttttcttt tcgaatgctt ttgcgcaggc gaagctttta
     1741 cgttttcccg atattcacgg cgaaaaagtc gtttttactt acggcggcga catctggacg
     1801 gctgccgcaa acggcggagc agcagcggcg acgcgtctga cggcgcacgc gggcgtagaa
     1861 gttttcgcca aattttcgcc cgacggaaac tggattgcct ttaccgggca atacgacggc
     1921 gacgagcagg tttacgtgat gcccgcgggc ggcggcgagc cgaaacagct gacgttttac
     1981 ccggcgaaag gaccgctcac gccgcgctgg ggttacgaca atcaggtgat gggctggac
//
LOCUS       DASWNE010001046         3554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_631036, whole genome shotgun sequence.
ACCESSION   DASWNE010001046 DASWNE010000000
VERSION     DASWNE010001046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631036"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /gene="rplI"
                     /locus_tag="VGB68_19955"
     CDS             <1..544
                     /gene="rplI"
                     /locus_tag="VGB68_19955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEY0051583.1"
                     /translation="ANTTVLLREDVDNLGGRGEIVKVKAGYARNYLLPRNIATLATKG
                     NVKKVENERAALLKKAATERETAEAQAEQMRDITLSFERKAGEQGVLFGSVTSMDVAQ
                     ALQAKGYEIDRRKITLKDAIKETGEYTVPVKLHREVTLNVPVIVTAEGGAAPVKEDAP
                     EAAGAPAENSEENSGETAEA"
     gene            729..2090
                     /gene="dnaB"
                     /locus_tag="VGB68_19960"
     CDS             729..2090
                     /gene="dnaB"
                     /locus_tag="VGB68_19960"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEY0051584.1"
                     /translation="MASTNTEFAREQFLEKPLPSSPESERVILGAILLDNTLISQAIE
                     LLSQDDFYSPLHRRVFRAMSNLFERGDKIDPILIGEELRKEGQLENIGGVATITNLTY
                     GLPHFSNIAHYAKIVHDKAVIRNLIKVCNNVVSEALSEEEDAEVVLDHAEQAIFALAD
                     ERTRQGFSHIKPVAETVLAKVQEYARRESHALTGLSTGFRDLDQMTSGLQQTDLIIVA
                     ARPSMGKTALCLTLAQNAATLENAVVAVFSLEMSKEQLVMRMLSSEAKVDAHRFRSGF
                     LTRDEWGRLAEAIGTLSEAKIFIDDTAGISVLEMRAKTRRLIAEQKKLDLIVVDYLQL
                     MSGSRRTESRQQEVSAISRELKGLAKELGVPVVALSQLSRAPEARNPPKPMMSDLRES
                     GSIEQDADVVAFIYRDDYYKPTEENAGIAELIISKQRNGPTGTVKLAFLKEFTRFENY
                     FGE"
     gene            2091..2618
                     /locus_tag="VGB68_19965"
     CDS             2091..2618
                     /locus_tag="VGB68_19965"
                     /inference="COORDINATES: protein motif:HMM:NF009807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-dependent hydrolase"
                     /protein_id="HEY0051585.1"
                     /translation="MSEDLRFPIGKFSFDAANAASGRVESIQIIEELPVRLRQAIAGF
                     NDEQLQTPYRDGGWTIHQLIHHIADSHMNSFIRFKLALTEDKPTIKTYEEADWAETTD
                     VSGVTADISLKLLDALHERWFILLASLSDEAFTRELNHPEHGLINLEYLTALYAWHSR
                     HHTAHITNLREKMNW"
     gene            2707..>3554
                     /gene="alr"
                     /locus_tag="VGB68_19970"
     CDS             2707..>3554
                     /gene="alr"
                     /locus_tag="VGB68_19970"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY0051586.1"
                     /translation="MSRPTWAEIHLDHLAFNLQSVKKRIGENIKYMAVVKADAYGHGA
                     LRCAQTLEKAGIDWLGVALPEEGVELRKNGIRKHILCLGSFWAGQENELLEYHLTPSI
                     FRLEIAEKFNRAAKERGVVADVHVKVDTGMNRIGVRFDEVREFAEELKKFTNLKVDGL
                     MTHFAAADNDDRFTNLQINRFNETIEIFHEKGFRPTYRDLANSPGAIGYENAKGNMVR
                     LGGILYGLWWDVLPKTVNPPDLKPVLTLHTKISLLKRVPKGETVGYNRTWKAEKDSLI
                     ATIPIG"
BASE COUNT          920 a          896 c          970 g          768 t
ORIGIN      
        1 ggctaatacc acagttttat tacgtgaaga cgtcgataac ttgggcggac gcggcgaaat
       61 cgtgaaagtc aaagccggtt acgcgcgcaa ttacctgctg ccgcgcaata ttgcgacgct
      121 cgccaccaaa ggcaacgtca aaaaagttga aaatgaacgc gcggcgctgt tgaaaaaagc
      181 ggcgacggaa agagagacgg cggaagcgca agccgaacag atgcgcgaca ttacgctcag
      241 tttcgaacgc aaagcgggcg agcagggagt tcttttcggc tcggtgactt cgatggacgt
      301 ggcgcaggca ttgcaggcga aaggttacga aatcgaccgc cgcaaaatca ctttgaaaga
      361 tgcgattaag gaaacgggcg aatacacggt tccggtcaaa ttgcaccgcg aagtgacttt
      421 aaacgtgccg gtcatcgtga cggcggaagg cggcgcggct ccggtcaaag aagatgcgcc
      481 ggaagctgcc ggcgcgccgg cggaaaattc cgaagaaaat tccggcgaaa ccgccgaagc
      541 ctagcattta ccggaaaaat aaatcggaga atttcctttg gttgtttatc cgttttcggt
      601 tattatctaa gactgcaaga ccataaatct tgcagtcttt tttttccggc aaaatctaaa
      661 actcagtttt tgtgtaaatc gagtcaaccc actggctgcc cgttaccgat tactgaatac
      721 ttgtgtaaat ggcgtcaaca aacacagagt tcgcgcgcga acaatttctc gaaaaaccgc
      781 tgccgagcag tccggaaagc gagcgcgtta ttctgggcgc gattctgctg gacaatacgc
      841 tcatctcgca agccatcgag cttttaagcc aggacgattt ttattcgccg ctgcaccggc
      901 gagtctttcg cgcgatgtcc aatcttttcg agcgcggcga taaaatcgac ccgattctca
      961 tcggcgaaga gctccgtaaa gaaggacaac tggaaaacat cggcggcgtg gcgaccatca
     1021 cgaatttaac ttacggctta ccgcactttt cgaacatcgc gcactacgcc aaaatcgtcc
     1081 acgacaaggc ggttatcaga aatctaatca aagtctgcaa caacgtcgtc agcgaagcgc
     1141 tttccgaaga agaagacgcc gaagtcgtgc tcgaccacgc cgaacaggcg attttcgcgc
     1201 ttgccgacga gcgaacgcgt cagggctttt cgcacatcaa gccggttgcc gaaaccgttc
     1261 tggcaaaagt tcaggaatat gcccgccgcg aaagccacgc cctgaccggg ctttcgaccg
     1321 gttttcgcga cctcgaccag atgacttccg gcttgcagca gacggatttg attatcgtcg
     1381 ccgcccgtcc cagcatgggc aaaaccgcgc tgtgtctgac tttagcgcaa aacgccgcga
     1441 cgctggaaaa cgccgtggtc gccgtttttt cgctggaaat gtcgaaagag caactggtga
     1501 tgcgaatgct cagctcggag gcgaaagtcg acgcgcatcg ttttcggagc ggttttctga
     1561 cgcgcgacga gtggggaaga ttagccgagg caatcggcac tttgtcggaa gcgaaaattt
     1621 ttattgacga tacggcggga atttccgttc tggaaatgcg cgcgaaaacg cggcgtctta
     1681 ttgccgagca gaaaaagctc gatttgattg tcgtcgatta tttgcagctg atgtccggct
     1741 cgcggcggac ggaatcgcgc cagcaggaag tttcggcaat ctcgcgcgag ctcaaaggct
     1801 tagccaagga attaggcgtt ccggtagtcg cgctttcgca attgtcgcgc gcgcccgagg
     1861 cgcgtaatcc gccgaaaccg atgatgtcgg atttgcgcga atccggcagc atcgagcagg
     1921 acgccgacgt cgttgctttt atttatcgcg acgattatta caaaccgacc gaagaaaacg
     1981 ccggaattgc ggaattgatt atttcaaaac agcgcaacgg tccgaccggg acggtcaagc
     2041 tggctttttt aaaggaattt accagattcg agaattattt tggagaataa atgagcgaag
     2101 atttacgttt tccgattgga aaattttcct ttgacgcggc gaacgcggcg agcggtcgcg
     2161 tcgagtctat tcagattatc gaggagctgc ccgtcaggct gcggcaggcg attgccggct
     2221 ttaacgacga gcaattgcag acgccgtacc gcgacggcgg ctggacgatt catcaattga
     2281 ttcatcatat tgcagacagc catatgaaca gtttcattcg tttcaagctg gcgctgaccg
     2341 aagacaaacc gacgataaaa acttatgagg aagccgattg ggcggaaact accgacgtct
     2401 cgggcgtgac ggcggacatc tcgctgaagc tgctcgacgc tctgcacgag cgttggttta
     2461 ttctgctggc ttcgctgtcg gacgaagcgt ttacgcgcga gctgaatcac ccggaacacg
     2521 ggcttatcaa tctggaatat ttgacggcgc tctatgcctg gcacagccgc catcacacgg
     2581 cgcacattac gaatctgcgc gagaaaatga actggtaatt taaaaggata aaggctgaag
     2641 gataaaggat aaagtaaaat cgattcctta gtttccagcc tttatccttt atcctttatc
     2701 ctttttatga gccgtccgac ctgggcagaa atacatctcg accatctcgc ctttaacctg
     2761 caatccgtta aaaaacgcat cggcgaaaat atcaagtata tggctgtcgt caaagccgac
     2821 gcctacggtc acggcgcgct ccggtgcgct cagacgctgg aaaaagcggg catcgactgg
     2881 ctcggcgtcg ccctgccgga agaaggcgtc gagctgagaa aaaacggtat tcgcaaacat
     2941 atcctgtgtc tcggaagctt ctgggcggga caggaaaacg agcttctcga atatcacctg
     3001 acgccgtcga ttttccgtct ggaaatcgcc gaaaaattca accgggcggc taaagagcga
     3061 ggcgtcgtcg ccgacgttca cgtcaaagtc gatacgggta tgaaccgcat cggcgttcga
     3121 ttcgacgagg ttagggaatt tgccgaagaa ttaaaaaagt ttacgaacct gaaagtcgac
     3181 ggcttaatga cgcattttgc ggcggctgat aatgacgacc ggtttacgaa tttgcaaata
     3241 aaccgcttta acgaaacaat cgaaattttt cacgaaaaag gatttcgccc gacctataga
     3301 gatttagcca actcgccggg cgcgataggc tacgaaaacg cgaaaggcaa tatggtgcgg
     3361 ctcggcggca ttttatatgg gttgtggtgg gacgttttgc cgaaaacggt taatccgccg
     3421 gatttgaagc cggtgctgac gctgcacacg aaaatttctc tgctcaagcg cgtgccgaaa
     3481 ggcgagaccg tcggctacaa tcgcacctgg aaagccgaaa aagactcgct gattgcgacg
     3541 attccgatcg gcta
//
LOCUS       DASWNE010001047         3228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_386971, whole genome shotgun sequence.
ACCESSION   DASWNE010001047 DASWNE010000000
VERSION     DASWNE010001047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386971"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..1238)
                     /locus_tag="VGB68_19975"
     CDS             complement(66..1238)
                     /locus_tag="VGB68_19975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051587.1"
                     /translation="MIFTLEALQAKHGDSLLLHYGTKDSPELVIIDGGPGGVFDISLR
                     PRLEQIKKKRSPDAPLPVNLMMVSHIDDDHINGILELTDLLIEQRDRREQLLCDITTL
                     WHNSFDEILGNDETAAIAASLGSVVKLSSAGDVEFPKNLFRGEPEAAIAASVLQGRKL
                     KNNREKLSILKNDPFESLVALPKKDKTPFSIEGNLKFTVLGPTELRLKNLQDDWNKKL
                     KEIQKKEAAQARALAAEFIDKSVYNLSSIIVLAEAGGKKMLLTGDALAADILEGLKEA
                     GVLKDGETLHIDLLKMPHHGSRANNTEEFLSSVTADHYVFSANGKHEHPSVETLEMIS
                     KARGSDAYTVHLTNAVPHAVEFYDADKKKPGRNYKVVIRRDPELSLSINLGEPYSD"
     gene            complement(1264..>3228)
                     /locus_tag="VGB68_19980"
     CDS             complement(1264..>3228)
                     /locus_tag="VGB68_19980"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEY0051588.1"
                     /translation="FKQLEEVIFGKTGFRRFTQDSSVLPDVWMDFGTEPDKQFDLLLT
                     PVWGKAPGKISNIIDERLKAERETERWNAVHRPQGEATEMAYNTSTIAVRLYFDEMIR
                     VILPLTQWWENYVLNDEGKYFSRVVDDADFRANLITALENSVVNPEETSAAPSPAAAA
                     GKLVWDINQAIKHKVWGDDKPKRIEHLVWLIKIVGAIILEDNGKKNDEILEEVKDIVN
                     AFVELMRDINQPVREEEKGLVWAINRNRNASAMIWQSTTAIKADAARRVFEVKCEGIR
                     WAIIDSGIDATHPAFRVRDLDGVAAKEPFRDNEGNEKNMTRIEKTYDFTLIRKILSRD
                     QRTIDSLPIQIQRRFGPGERKTLQERLNQGRAIDWEFLAPLLEIKHTDKDNEYKAPVF
                     EHGTHVAGIIAADWKSTDVTNEKKINIQGVCPDLRLYDLRVLDENGSSDEFSVLAALQ
                     FIQYLNTNSDYLLLHGVNLSLSIRHSVKNFACGCTPVCEEAERLINAGLIVVAAAGND
                     GYLEAEMSNDRVSEGYRSISITDPGNAQGVITVGATHRDSPHTYGVSYFSSRGPTGDG
                     RIKPDLVAPGEKITSTVPENGLKTMDGTSMAAPHVSGAAALLIARYRELVGKPARVKQ
                     ILCDTATDLGRERYFQGSGMVDILRAMQSV"
BASE COUNT          646 a          905 c          820 g          857 t
ORIGIN      
        1 cgtttccact gatacaggcg aacgagtcga ggcgcttatc gctgccggat taaacggagc
       61 gggcattaat cgctatacgg ctcgccgagg tttatgctca gcgatagttc cgggtcacgg
      121 cgtataacaa ccttgtaatt cctgcccggt ttctttttat ccgcatcata aaattcgacc
      181 gcgtgcggta cggcgttcgt cagatgaaca gtgtaggcgt cgctgccgcg cgctttcgaa
      241 atcatttcga gtgtttccac gctcggatgc tcgtgtttcc cgtttgccga aaatacataa
      301 tggtcagccg ttacggagct gagaaattct tccgtattat tggctcggct tccgtggtgc
      361 ggcattttga gcaaatcgat atgtagagtt tcgccgtctt tcaaaactcc cgcttctttc
      421 aagccttcca gtatatcggc ggcaagcgca tcgccggtga gcagcatttt tttgcctccg
      481 gcttcggcga gaacaatgat gctcgagaga ttatagaccg atttatctat aaactcggcg
      541 gcgagagcgc gagcctgcgc cgcttctttc ttttgaatct ctttgagctt tttgttccaa
      601 tcgtcctgaa gatttttaag ccgcagctcg gtcggaccga gcaccgtaaa tttcaaattt
      661 ccctcgatgc tgaacggagt tttatccttt ttcggcaggg cgacgagact ttcaaacggg
      721 tcatttttca atatagacag tttctctctg ttgtttttca gcttgcgtcc ctgcaacacg
      781 ctggcggcaa tagcggcttc cggctcgccg cgaaacaggt ttttcggaaa ttctacgtcg
      841 cccgccgacg aaagcttgac taccgagccg agcgacgctg caatcgccgc ggtttcgtcg
      901 tttccgagaa tttcgtcaaa gctgttgtgc catagcgtcg tgatgtcgca taggagctgt
      961 tcccgccggt cgcgctgctc gataagcaaa tccgtcaact cgagtatgcc gttgatatgg
     1021 tcgtcgtcga tatggctgac catcatcaga tttaccggaa gcggcgcatc ggggctgcgt
     1081 ttctttttaa tttgttcgag ccgcggacgc aggctgatgt caaaaacgcc gcccggaccg
     1141 ccgtctataa taaccagctc gggcgaatcc ttcgttccgt aatgaagcag aagcgaatct
     1201 ccgtgctttg cctgcagcgc ttcaagcgta aaaatcattt tattcctcct cgaatatgac
     1261 tcatcagacc gattgcatag cccgcaggat atcgaccatg ccgcttccct gaaagtaccg
     1321 ctcgcgtcca aggtcagtcg ccgtatcgca caaaatctgt ttcacgcgag ccggctttcc
     1381 gaccaattcc cggtagcgcg caatcaaaag cgccgccgcg ccgctgacgt gcggcgcagc
     1441 catgctcgtt ccgtccattg tcttcagccc gttttcagga accgtgcttg taattttttc
     1501 gcccggggcg accaggtcgg gcttgatgcg cccgtcgccg gtcggtccgc gactggaaaa
     1561 atagctgact ccgtatgtat gcgggctgtc gcggtgcgtt gcgccgaccg tgatgacgcc
     1621 ctgcgcgttg cccgggtcgg tgatgctgat gctcctgtag ccttcggaaa ctcgatcgtt
     1681 cgacatttcc gcttcgagat agccgtcgtt gcccgccgcc gcgacgacga taagccccgc
     1741 gtttatcaat cgttcagcct cttcgcagac gggcgtgcag ccgcaggcaa agtttttgac
     1801 gctatggcga atcgagaggc ttaaattcac gccgtgcaac agaagatagt cgctgtttgt
     1861 gttcagatac tggataaact gcaaagcggc gaggacggag aattcgtcgc tgcttccgtt
     1921 ttcgtcgagg acgcgcaggt catacaggcg caggtcgggg cagacgcctt gaatgttaat
     1981 ctttttttca ttcgtcacgt ccgtgctttt ccaatcagcg gcgataatgc ccgcgacgtg
     2041 cgtcccgtgc tcaaaaacgg gagccttgta ttcgttatct ttatccgtat gcttgatttc
     2101 cagcagcggc gcgagaaact cccaatcgat ggcgcgcccc tgattcagac gctcctgcag
     2161 agtctttctt tccccgggac cgaaacgcct ttggatttga atgggcaggc tatcgatcgt
     2221 cctctggtcg cggcttaata ttttgcggat gagcgtgaag tcgtaggttt tttctatccg
     2281 cgtcatattt ttttcattgc cttcgttgtc cctaaaaggc tctttagcgg cgacgccgtc
     2341 caaatctctc acgcgaaagg ctggatgtgt cgcatcgatg ccgctgtcga taatcgccca
     2401 tctgatgccc tcgcacttga cttcaaaaac ccttcgcgcg gcatcggctt taatggcggt
     2461 cgtcgactgc caaatcattg ccgacgcgtt gcgattacga tttatcgccc acaccaatcc
     2521 cttctcttct tccctgaccg gctggtttat atctctcatc agctcgacga aagcattgac
     2581 aatatccttg acctcttcga gtatttcatc attttttttg ccattgtcct ccagaatgat
     2641 cgccccgact atcttaatca gccaaaccag atgttcgatg cgtttcggct tgtcgtctcc
     2701 ccacacttta tgcttaatcg cctgattaat gtcccagacc agctttccgg cagcggcagc
     2761 aggagaagga gccgcagaag tctcctcggg attcacgacc gagttttcca gcgcggtgat
     2821 aagattggct ctgaaatcgg catcgtcgac gactctggaa aagtatttac cttcgtcatt
     2881 aagtacgtag ttttcccacc attgcgtcag cggcaggatg acgcgaatca tttcatcgaa
     2941 ataaagccgg acggcgatgg tcgaggtatt gtaagccatc tcggtcgcct ctccctgcgg
     3001 acgatgcacc gcgttccacc gctccgtttc ccgttcggct ttgagccgtt cgtcgataat
     3061 gttggaaatt ttgcccggag ccttgcccca gaccggcgtc agcagcaggt cgaactgctt
     3121 gtccggttcg gtgccgaaat ccatccagac gtccggcaga acggacgaat cctgtgtgaa
     3181 acggcgaaag ccggtctttc cgaaaattac ttcctctaat tgtttgaa
//
LOCUS       DASWNE010001048         5919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_387023, whole genome shotgun sequence.
ACCESSION   DASWNE010001048 DASWNE010000000
VERSION     DASWNE010001048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5919
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387023"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VGB68_19985"
     CDS             complement(<1..273)
                     /locus_tag="VGB68_19985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility protein PilT"
                     /protein_id="HEY0051589.1"
                     /translation="MTDLGGSDLHLTTNSSPQVRVHGHLHQLGEYPPLTPAQTKQLAY
                     SVLTDAQKHRFEEDLELDFSFGLKGMSRFRANLFNQKGAVGAVFRAI"
     gene            complement(422..1240)
                     /locus_tag="VGB68_19990"
     CDS             complement(422..1240)
                     /locus_tag="VGB68_19990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051590.1"
                     /translation="MLRAIALSLALLVSLGAIIPVATNHSEAGPRYHKKKKKKLKKYS
                     KAWWKWYRAKQRRQKALAMRKRALRLRQIRMAKENGVQGDPISKPAISKTDKSLKSAR
                     KQEDSMPQLLPSGETAPKGWKKNQATQGELQFRVDDDSGKTIGTASLGVVGPAVGGDN
                     VAGRNKTVGGVQTSALRRTVIDRMMKEQGWVVNDYQKEVGGKKVYVVVAQSPGTNGQI
                     QSRTFYFAEVEGRIYSLATNASDTSSERIAQESEKVLNSLQQRTNRPVQQAELR"
     gene            complement(1256..3088)
                     /gene="pilB"
                     /locus_tag="VGB68_19995"
     CDS             complement(1256..3088)
                     /gene="pilB"
                     /locus_tag="VGB68_19995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEY0051591.1"
                     /translation="MSAKLGEILVRENLITPQQLREALEYQRDKGGRLGSNLVKLDFI
                     SDDVITAVLSRQYGVPSINLDLFHIEQETINLISQEVALKYSVLPISKVGATLTLAMA
                     DPTNVFAMDDIKFMTGLNVEPVIASETSIHAAVGRYYSSSKSIEVVGVSDRFEKVTAK
                     FSKNGNGGSNGATRGEQRLVESDLTVSLDRFQFDKDAGGEFEVLEENEEIDLAELAKA
                     SEDAPVVRLVNVLLVDSLRRGASDIHIEPYEKDFRIRFRIDGVLYDVMHPPMKMRDAL
                     ISRLKIMAKLDISEKRLPQDGRIKIKVKIDARSRELDFRVSSLPTLFGEKVVLRLLDK
                     DKLMLDMTKLGFEQQSLEIFQRNISKPYGMVLVTGPTGSGKTNTLYSALQALNTSETN
                     IMTAEDPVEFNLPGINQVQMKEQIGLNFAAALRSFLRQDPNIILVGEIRDFETAEIAI
                     KAALTGHMVLSTLHTNDAPSTISRMVNMGIEPFLVATSVNIIQAQRLIRRVCKECKEE
                     TQLPPEALVEIGFDEDEVRQIKTYKGRGCQVCNGTGYKGRVGLYEVMEVTDELRELII
                     IGASAIELRKKAIELGMITLRQSGLYKIREGITTIEEVVKETVL"
     gene            complement(3395..4702)
                     /locus_tag="VGB68_20000"
     CDS             complement(3395..4702)
                     /locus_tag="VGB68_20000"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0051592.1"
                     /translation="MYRKNSIVSLFIIALFLTGSLVAFAQSTAPVRGKVQLKKADNTI
                     VPLSDADVTAYRTDISKGKLPVAKTDKKGYFSFAGVPYAQTFVLVVTAPNITAGYFPN
                     VKANMDNIIIEVSEGDGKIPTEEEIRGALASVTTTGELTPEEKKKQEDLVKKNAEIEA
                     KNKRIQNANEVINRAIAEGKKAYDASDWDMAITRFDEGYNADPEFPGTAPVFLNNKAD
                     ALKQRGISNYKKATTDAANKPALMEAMKKDFLESITAAQRAVDILKNANSAEANVQKG
                     YVTNRMVAYSIMNDVYRLLIETRADESKAQEAVQVVDAYVAIEPDAAKKATAQIKMAD
                     VLRKIGKSDEAIPLYRKALESAPDNADLLAGLGLSLFNSGVIANNKEQMQEGLNIMER
                     FTATAPDTHALKASVKEAVDYLKNQEKLTPQKTTKPATTKKRT"
     gene            4967..5791
                     /locus_tag="VGB68_20005"
     CDS             4967..5791
                     /locus_tag="VGB68_20005"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0051593.1"
                     /translation="MRFTVLGSGSTGNAVLITAGKTKVLVDAGLSAKEILRRLACVGE
                     DCAQIDAILITHEHSDHASGLRVLLKSLKCPVFISGATRDAYLREKPNLTNNEPQKRA
                     DAVRNRTVEIDSGKDFRIGEIDFHPFSVPHDAVDNFGFVAEHNGVKVATLMDFGCITT
                     LIKEKLSNCDGIVIESNHSRDMLRACAVYTWELKQRILSRNGHLSNEDLCDWLTNDFD
                     GSARHIVLAHLSQRANEPHLARLMAETALQLRAPLFKCETKITLSRPKEPTEWIEF"
BASE COUNT         1351 a         1493 c         1470 g         1605 t
ORIGIN      
        1 aatcgcgcga aaaaccgcgc ctaccgcacc tttttggtta aagagattgg cgcggaaacg
       61 cgacattcct ttcaaaccga acgaaaagtc gagttcgagg tcttcttcaa aacggtgttt
      121 ttgcgcgtcg gtcaaaaccg aataggcaag ctgtttggtc tgcgcgggcg tcagcggcgg
      181 atattcccca agctggtgca gatgaccgtg gacgcgcacc tgcggcgacg aattggtcgt
      241 caaatgcaaa tccgagccgc ccaggtccgt catttttcgg agcaggtcgg ggagcgttac
      301 ttgcgattca ggattttgtt gctcgtagct cataattttt gatttttgag tgatgtgtcg
      361 agaatcggga gtcgagagtt aaaacaaaga ctcgcgactc ctgactctcg attgtgttga
      421 cttatctaag ttcggcttgc tgaaccgggc ggttcgtgcg ttgctggaga gagttaagga
      481 ctttttcgga ttcctgcgcg atacgctccg aggaggtatc cgaagcgtta gtcgccagac
      541 tgtagatgcg tccttcgact tcggcgaaat agaaggtgcg tgattgaatc tgaccgttcg
      601 tgccgggcga ttgggcgacg acgacgtaaa cttttttgcc gccgacctct ttctggtaat
      661 cgttgacgac ccagccctgc tctttcatca tgcggtcaat caccgtgcgg cgcagcgcgc
      721 tggtttggac gccgccgacg gttttgtttc gtccggcgac gttatcgccg ccgacggcgg
      781 gaccgacgac gccgagcgat gccgtgccga tggtttttcc gctgtcgtca tcgacgcgaa
      841 attgcagctc gccctgtgtc gcctgatttt tcttccagcc cttcggagcg gtttcgcccg
      901 acggcaacag ctgcggcatc gaatcttcct gtttcctggc agatttcaga gatttatccg
      961 ttttagaaat agccggttta gaaatcgggt cgccctgcac gccgttttcc ttcgccatcc
     1021 gtatctgacg cagacgcaaa gcgcgtttgc gcatagccag cgctttctgc cgacgctgct
     1081 tggcgcgata ccatttccac caggctttcg aatatttttt cagttttttc tttttctttt
     1141 tatggtatcg cggtccggct tccgaatgat tggtcgctac cggaataatc gctcccagcg
     1201 acaccaacaa agctaacgat aatgctattg ctcgcaacat acttcctcca attcgttaaa
     1261 gaaccgtttc cttaactact tcctcaatcg ttgttattcc ttcacgtatt ttataaagac
     1321 cggattggcg caaagttatc atcccgagtt cgatagcttt tttgcgcaat tcgatggcgc
     1381 tcgcgccgat aataatcaat tcccgcagct cgtccgtcac ttccatcacc tcgtaaagac
     1441 cgacgcgacc tttgtagccg gtgccgttgc agacctggca gcctctgcct ttgtaagttt
     1501 tgatttggcg aacttcgtct tcgtcaaatc cgatttcgac gagcgcttcg ggcggcagct
     1561 gagtttcttc cttgcattcc ttgcagacgc ggcggataag tctctgcgcc tgaatgatgt
     1621 taacgctcgt cgccaccaga aacggctcga tacccatatt gaccatacgg gaaatcgtcg
     1681 aaggcgcgtc gtttgtgtgc agggtcgaaa gcaccatatg acccgtcaac gcggctttga
     1741 tggcgatttc cgccgtttca aaatcgcgga tttcgccgac cagaatgata ttcgggtcct
     1801 ggcgcaggaa agaacgcagc gcggcggcga aattcaagcc gatttgttct ttcatctgca
     1861 cctgattgat gcccggcagg ttaaactcga ccgggtcttc cgccgtcata atattcgttt
     1921 cggaagtatt taaggcttgc aatgccgaat aaagcgtgtt cgttttaccc gaacccgtgg
     1981 gacccgtcac cagaaccatc ccgtaaggtt ttgagatatt gcgctggaaa atctccaaac
     2041 tctgctgttc gaaaccgagc ttggtcatat cgagcatcag tttatcttta tccagcaggc
     2101 gaagcacgac tttttcgccg aacagcgtcg gcaggctcga aacgcgaaaa tccagctcgc
     2161 gcgagcgcgc gtcaatctta accttaatct tgatgcgtcc gtcctgcggc aggcgtttct
     2221 cggaaatgtc gagcttcgcc ataattttca gacgcgaaat caacgcgtcg cgcatcttca
     2281 tcggcgggtg catcacgtca tataaaacgc cgtcgatgcg aaagcgaatg cgaaaatctt
     2341 tttcgtacgg ctcgatgtga atatcggatg cgccgcgccg caagctgtca accagaagaa
     2401 cgttgaccag acgcacgacg ggagcgtctt cgctcgcttt ggcgagctcg gcaaggtcga
     2461 tttcctcgtt ttcttcaaga acttcaaact cgccgcccgc gtccttatca aactgaaaac
     2521 ggtcgagcga gaccgtcaaa tccgattcga ccagtcgctg ctcgcccctc gtcgccccgt
     2581 tggagccgcc gttgccgttt ttggaaaatt tcgcggtgac tttttcgaaa cggtcgctga
     2641 cgccgacgac ctcgatggat tttgaagagc tgtaatatct gccgacggcg gcgtgaatcg
     2701 aggtttccga ggcgatgacc ggctcgacat tcagcccggt cataaatttt atgtcgtcca
     2761 tcgcaaagac gttggtcggg tcagccatcg ccagcgtcag agtcgcgccg actttggaaa
     2821 tcggcagcac ggaatatttc agcgcgactt cctgcgaaat cagattgatg gtttcctgct
     2881 cgatgtgaaa caaatccaga ttgattgacg gaacgccgta ttgccgcgac aaaacggcgg
     2941 tgatgacatc gtccgagata aaatcgagct tgactaaatt tgaccctaaa cgcccgcctt
     3001 tatcacgttg atattccaac gcctcgcgca attgttgtgg agttattaaa ttttcgcgga
     3061 caagaatttc tccgagttta gctgacatcg atttatttta tagattcatt aagtcgagaa
     3121 tttttaagac ttacaattga gaaaggaaac gcgggctgtc tattttaata aagctttgaa
     3181 gacagtgctg gcaacgatga taaacatcgt tgattgttta tagtatatgt ttaactacaa
     3241 agtgtcaaga tttcttttgg aaaaaatttg ggataaaagt cgggaaaaca aaagattttc
     3301 cggcggcgat ttatagggcg aaattaaaag gctgttcctg aataaattaa attcaggaac
     3361 agcctttcgt aatatttagc gtttcggcga aaacttaagt tctcttttta gtcgtcgccg
     3421 gcttggtcgt tttctgcgga gtcagttttt cctgattttt cagataatcg accgcttcct
     3481 taacgctggc tttcagagcg tgcgtatcgg gcgcggttgc cgtaaaacgc tccataatat
     3541 tcaagccttc ctgcatctgt tccttattgt tggcgatgac gccggaatta aacagggaca
     3601 agcctaatcc ggcaagcaaa tctgcgttgt cgggcgcgga ttcgagcgct tttcggtaaa
     3661 gcggaatcgc ttcgtcggat ttaccgattt tgcgcagcac gtcagccatt tttatttgcg
     3721 cagtagcctt tttggcggcg tccggttcga tggcgacata agcatctacg acctgaaccg
     3781 cttcctgcgc tttggattca tcggctctgg tttcaatcaa taaacgataa acgtcgttca
     3841 taatcgaata cgcgaccatc cggttcgtga catagccttt ctggacattg gcttcggcgg
     3901 aattcgcgtt ttttaaaata tcgaccgctc tttgagccgc agtaatcgat tccagaaaat
     3961 cttttttcat cgcttccatc agggcgggtt tattggcggc atcggtggtt gcttttttgt
     4021 aattgctgat gccgcgctgt tttaaggcgt cggctttatt gttcagaaaa accggcgcgg
     4081 ttcccggaaa ttcggggtcg gcgttataac cttcgtcaaa tctggtgatt gccatatccc
     4141 aatcgctggc gtcataggct ttcttgcctt cggctatcgc gcggttgata acttcattgg
     4201 cattttgaat tctcttgttt ttggcttcta tttccgcatt tttcttgacc aggtcttcct
     4261 gttttttctt ttcttcggga gttaattcgc cggtcgtcgt aacgctcgcc agcgccccgc
     4321 gaatttcttc ctcggtcgga atttttccgt cgccctccga tacctcgatg atgatattat
     4381 ccatattggc tttgacgttc gggaaataac cggcggtgat attcggcgcg gtcactacca
     4441 gcacgaaggt ctgcgcgtag ggaacgcccg caaaactgaa ataacctttt ttatcggttt
     4501 tcgccaccgg cagcttacct tttgaaatat cggtgcggta cgccgtaaca tcggcgtcgg
     4561 aaagcggaac gattgtatta tcggcttttt taagctggac ttttccgcga accggcgcgg
     4621 tgctctgggc aaaagcgacc agacttcccg ttaaaaacaa ggcaataata aataatgaaa
     4681 caatagagtt tttacgatac atttgaatcc tccaatggta ttcgggacaa gtgttgaact
     4741 tgtgctctaa gatgaacgaa gcaaaaaaat agttgttaaa ctttgctgat tttaccttaa
     4801 agacgcggaa aatttaagta taaaatcaat ctccgacgcc ggaagcggcg catttttttc
     4861 ttttctgtgc taatctgtcg tttttgaaag gcgagttcaa acgaaaagac aaagaaagtt
     4921 tatttcttta ttttcgcaaa agatttcctt tcttccacta acgccgatgc gatttacggt
     4981 tctcggaagc ggttcgacgg gcaatgccgt tctcatcacc gccggaaaaa caaaagtttt
     5041 ggtcgatgcc ggattgagcg caaaagaaat tttgcggcga ctcgcctgcg tcggcgaaga
     5101 ctgtgcgcaa atcgacgcca ttttaatcac gcacgagcac agcgaccacg ccagcggctt
     5161 gcgcgtgctg ctgaagagtt taaaatgtcc ggtttttatt tccggcgcga cgcgcgacgc
     5221 gtacctgcgc gaaaagccga atcttaccaa caacgagccg caaaaacgcg ccgacgcggt
     5281 gcgaaaccgc acggtcgaga ttgattcggg caaggatttc cgcatcggcg agattgattt
     5341 tcacccgttt tcagtgccgc acgacgcggt tgataatttc ggcttcgtcg ccgagcacaa
     5401 cggcgtcaaa gtcgccactt taatggattt cggctgcatc acgacgctga taaaggaaaa
     5461 actgtcgaac tgcgacggca tcgtcatcga atcgaatcac agccgcgata tgctgcgcgc
     5521 gtgcgcggtt tacacctggg aattgaagca gagaatcttg tcgcgcaacg ggcatttatc
     5581 gaatgaagat ttgtgcgact ggctgacaaa cgatttcgac ggctccgcgc gtcacatcgt
     5641 tttggctcat ttatcgcaac gcgccaacga gccgcatctg gcgcgcctga tggctgaaac
     5701 cgccttgcag cttcgcgcgc cgcttttcaa atgcgaaacc aaaatcacgc tctcgcgccc
     5761 gaaagagccg accgaatgga ttgagttttg atgcaaaacg tttataaatg caaagcgcaa
     5821 agtgcaaaat gaaagaacgg atttaattct aaatcccgaa tcctaaatcc gtatattttg
     5881 catcttgcat tttgcgcttt gcattagtta cgcgttccg
//
LOCUS       DASWNE010001049         3124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_346256, whole genome shotgun sequence.
ACCESSION   DASWNE010001049 DASWNE010000000
VERSION     DASWNE010001049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346256"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="VGB68_20010"
     CDS             complement(<1..971)
                     /locus_tag="VGB68_20010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEY0051594.1"
                     /translation="MHTNLRSFLETLRRENDLIEIAAEVDPYLEIAEIHRRVIDEQGK
                     ALFFKNVKGSAYPVVTNLFGTNKRIELAFTRKPQEFVKTAVELVDKILPPKPKELWAY
                     RSMAFDALKLGTKKVKNAPVLEATDQPADLNKLPLLQLWHEDGGHFVTLPLVYTESPT
                     SGKHNLGMYRIQRYDAQTTGIHWQIHKGGGFHYHEAEKLNQSLPVTIFLGGAPAMILA
                     AIAPLPEDVPELMLASLLAGNKIKTVENPLNNHHRLIAEAEFAICGRVAPHTRKPEGP
                     FGDHYGYYSLQHDYPIFEAEAVFHRKDAIYPATVVGKPRQEDFYIGD"
     gene            complement(1189..2694)
                     /locus_tag="VGB68_20015"
     CDS             complement(1189..2694)
                     /locus_tag="VGB68_20015"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY0051595.1"
                     /translation="MKRIKVSGLSIKQAIFAFLLLPFAFSVVSAQTEMPPAPSAPRPV
                     QIPAVQEKSLSNGMTIAVVERRNVPLVTIQFLVKSGAESEAAGKAGLANMTAALLTKG
                     TKTRTATQIAEEMEFLGGSITAGAGWNNSTVTVTAMSDKIDAAMAILADVVLNPAFKQ
                     EEIDLLKSQTLDNLTYNLKQPGFLASYVASRYSFGEHPAGGTPASLKAISREDITNFH
                     SGSYGTSLSVLIFTGDITAARAEAIAKKYFGSWNNKTTYKLAPSSSAPVKSDTLLKRI
                     LVVDLPNSGQAAVSYSKKLQGASRLENNYYYPASVLNSLLGGGYSSRLNQEIRIKRGL
                     SYGAGSGFVWRRDKSNFGTRTQTKNESAAIVAELVLAEIKKLTESSIEDVELNPRKSV
                     LTGNFSRNLETTGGLAAQIAELYSFGLRPSQLNDYMNSVQTVSDKQIREFAGGNLTGG
                     DVIIVGDYAVFKDDLAKRFPNMPIEIIKADDLDLSNENLRRSGGEAVKTGS"
     gene            complement(2714..>3124)
                     /locus_tag="VGB68_20020"
     CDS             complement(2714..>3124)
                     /locus_tag="VGB68_20020"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEY0051596.1"
                     /translation="LAQEAAFTGDIRVDRGLLYFYAIASEGKTAEELEKSLLAELQKI
                     QAAPVSAKELEKAKNQLITRKVQELETNDGKAIAVERAVAYEGDPKAVNSDIRKLQAV
                     TAADVQRVMKKYFTDKNRVVIYYRNEGNSDADKK"
BASE COUNT          696 a          824 c          803 g          801 t
ORIGIN      
        1 taatcgccga tgtaaaaatc ttcctgtcgc ggcttgccga ccacggtcgc cggataaatc
       61 gcgtctttgc gatgaaaaac cgcttccgcc tcgaaaatcg ggtaatcgtg ctgcagggaa
      121 taataaccgt aatggtcgcc gaaaggacct tccggttttc gcgtgtgcgg cgcgactcgt
      181 ccgcagatgg cgaattcggc ttcggcaatc agacgatgat gattgtttaa aggattctcg
      241 acggttttta ttttatttcc cgccagcaag gacgcgagca tcaattcggg aacgtcttcg
      301 ggcagaggcg caatcgcggc gagaatcatc gcgggcgcgc cgcccagaaa aatcgttacg
      361 ggcaaagatt gattcagctt ttccgcttcg tgataatgaa agccgccgcc cttgtgaatc
      421 tgccagtgaa tgccggtcgt ctgcgcgtcg tagcgctgga tgcgatacat tccgagattg
      481 tgttttcccg atgtcgggct ttccgtgtaa accagcggca gcgtgacgaa atgcccgccg
      541 tcttcgtgcc agagctgcaa aagcggtaat ttatttaaat cggcgggctg gtcggtcgct
      601 tccagaaccg gcgcgttttt gacttttttt gtgccgagct tgagagcgtc gaaagccatc
      661 gagcgatacg cccacaactc tttcggcttg ggcggcaaaa ttttatcgac cagctcgacc
      721 gccgttttga caaactcctg cggctttcgc gtaaaggcaa gctcgatgcg tttgttggtg
      781 ccgaaaagat tggtgacgac cggatacgcc gagcccttga cgtttttaaa aaacagagct
      841 tttccctgct cgtcaatcac gcggcggtga atttcggcaa tttccagata cgggtcgact
      901 tccgccgcaa tttcgattaa atcattttcc cggcgcagcg tttcgagaaa ggaacgcaga
      961 ttcgtgtgca taaatctatt gaaacaaagg ctttttcaaa tgacaacccg ttctaaaaca
     1021 aaaaccgcaa ataagctcgt aaaacaaaac ttatttgcgg ttacaatttg acgcgggaaa
     1081 gacccgaacg cgaatcttcg gaacgacgaa cgatgagttc gattctctat atccgcaatt
     1141 catcattcat cgtttaagaa tactctccat cattgaaacg aaataaaatt acgagccggt
     1201 tttgaccgct tcgccgccgc tccggcgtaa attttcgttg cttaaatcta ggtcgtcggc
     1261 tttgatgatt tcgattggca tattcggaaa acgctttgcc aaatcgtctt taaagacggc
     1321 gtaatcgccg acgatgataa cgtcgccgcc ggtcagattg ccgcccgcga attcgcggat
     1381 ttgcttgtcc gaaaccgtct gtacgctgtt catataatcg ttgagctggc tgggacgcaa
     1441 accgaagctg tataactcgg cgatttgcgc cgccagcccg ccggtcgttt ccagattgcg
     1501 cgagaaattg ccggtcaaaa ccgatttgcg cggattcagc tcgacgtctt caatcgagct
     1561 ttcggtcagt tttttaattt cagccagcac taattcggca actatcgccg ccgattcgtt
     1621 tttggtctgc gtgcgcgtgc cgaaattgct tttatcgcga cgccagacga agccgctgcc
     1681 cgcgccgtag ctcaagccgc gcttgatacg aatttcctga ttcagacgcg acgaataacc
     1741 gccgccgagc agcgaattta aaacggacgc cgggtaataa taattatttt ccagacgaga
     1801 agcgccctgc aattttttgg agtagctaac tgccgcctga ccggagttcg gcaaatcgac
     1861 gacgagtatt cttttcagca gcgtatcgct ttttacgggc gcagaagaag acggggcgag
     1921 cttgtacgtg gttttattat tccaactgcc gaaatatttt ttggcgatgg cttccgcccg
     1981 cgccgccgta atatcgccgg tgaaaatcaa aacggacaaa ctcgttccgt aagacccgga
     2041 atgaaaatta gtaatatctt cgcgcgaaat cgcctttagg ctggcgggcg tgccgcctgc
     2101 cggatgctcg ccgaagctgt aacgactggc aacataactc gccagaaagc cgggctgttt
     2161 caggttataa gtcagattgt cgagcgtctg cgatttgagc aggtcgattt cctcctgttt
     2221 gaaagcggga ttcaaaacca cgtccgccaa aatcgccatc gcggcgtcga ttttgtcgct
     2281 catcgcggtg acggtgacgg tcgagttgtt ccagcccgcg cccgccgtta tcgagccgcc
     2341 gagaaattcc atctcttcgg cgatttgcgt tgccgtgcgc gttttcgttc ccttggtcaa
     2401 aagcgcggcg gtcatattcg ccaagcccgc ttttccggcg gcttccgatt cagcgccgct
     2461 tttgaccaga aactgaatcg tgacgagcgg aacattccgg cgctcgacga ccgcaatcgt
     2521 cataccgttt gacaaacttt tttcctgaac ggcgggaatc tgcacgggac gcggcgcgct
     2581 cggcgcgggc ggcatctcgg tctgcgcaga cacgaccgaa aaggcaaaag gcaaaagtaa
     2641 aaaggcaaaa atcgcttgct tgatacttaa acccgaaact ttgattcttt tcataatttt
     2701 tcctttttct cttttacttt ttatcggcat cgctgtttcc ttcgttccgg taataaatca
     2761 cgacgcggtt tttgtcggtg aaatattttt tcatcacgcg ctgcacgtcg gcggcggtga
     2821 cggcttgcag ctttcgaata tcggaattaa ccgctttcgg gtcgccctcg taggcgacgg
     2881 cgcgctcgac cgcaatcgct ttgccgtcgt tcgtttccag ctcctgaacc ttgcgcgtaa
     2941 tcaattgatt tttcgctttt tcgagttctt tggcggagac gggcgcggct tgaattttct
     3001 gcagttcggc gagcagagat ttttccagct cttcggcggt tttgccttcc gaagcaatcg
     3061 cgtagaaata aagcaagcct ctatcgacgc gaatgtcgcc ggtaaacgcc gcttcctgcg
     3121 cgag
//
LOCUS       DASWNE010001050         3679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_631263, whole genome shotgun sequence.
ACCESSION   DASWNE010001050 DASWNE010000000
VERSION     DASWNE010001050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631263"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1306
                     /locus_tag="VGB68_20025"
     CDS             <1..1306
                     /locus_tag="VGB68_20025"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY0051597.1"
                     /translation="FSNNNLSGNWYAQVVDRQTGGSLPAPNTTNLKNFRGNWWGTASP
                     VVTTANSAEPGYAAQIPVAYGGTATAPGGQPDIAGAASANIKYMPLLTNGGDTNIETT
                     PGRGTFGFQGARSVVSVKPSMSNGWSAVSQRTASGSFVNGPGMPPLGSGSYRMMLGAG
                     NSGPDLPQGGAGQGGKTWLTTQQFDNTLLSGISVLGYSTYVAASPSSAGNIITPTLQL
                     QIDLDGNGTRDSTMIFEPYYSTAAGGGTQSNVALGQWQTWDARAGKWWFNNATVFGCG
                     QCAFPTFDAIIAAYPNAKIVTWYALADGYGTQLVAGQNSAGSPWANFDGNIDAFTITV
                     NAPNTTFDFEPLAPTAASVSIAGQVLTATGQSIPNAKIFLTDNQGVKRVAVSNAFGHY
                     GFEGITVGETYILSVVSKRYTFTPRAVEVNEELTALNLIAEL"
     gene            complement(1376..1588)
                     /locus_tag="VGB68_20030"
     CDS             complement(1376..1588)
                     /locus_tag="VGB68_20030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YwbE family protein"
                     /protein_id="HEY0051598.1"
                     /translation="MDGKQRKDVRPGLRVAIVLKQDQRTGRQTVGTVKDLLTNSAFHP
                     HGIKVRLTDGQVGRVQEILTSDSPES"
     gene            1709..2536
                     /locus_tag="VGB68_20035"
     CDS             1709..2536
                     /locus_tag="VGB68_20035"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY0051599.1"
                     /translation="MLDNLVESKSNAGETGRRAGFLVTTLFTVVSLFLMSLTYSLFAK
                     DLGTDDNGLELSTLVAPVPEAETPPQPELQKQPERRAASTQSNIVMRKEAFAPIEQNT
                     KPPADTNGQKGVQSWVKGAKLGSADLTASGQPNSPARSNNEPGEGINGNNVSTEKEVE
                     PPPMLTKPSPKPPVEQPKKEKPPVVTGGVVNGKAINLVKPQFSAAAKAIRASGAVNVQ
                     VLIDEKGNVVSASAVSDHRLLRQSAESAARASKFTPTFLTGQPVKVTGVIVYNFALQ"
     gene            complement(2621..>3679)
                     /locus_tag="VGB68_20040"
     CDS             complement(2621..>3679)
                     /locus_tag="VGB68_20040"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEY0051600.1"
                     /translation="QQGKMAVVCNAGCLVQPLTRATYQAGAPRPYQLFSHPDQAEQFK
                     TGISSFKSTTGWGGRTADRTSTLNPGGAVPMITSIAGASVFNNGASTKPLIVGAAPTP
                     LNQVLTLNGFGTATDELARLAAMNSARTRDLNYTLVQQASAITQQAVNVSQQLSVDPT
                     LTVTFPATSLGNQLRQVAKLIKLRTLLGMSRQIFFVQIGGFDHHSNQLGNHDNLLTQV
                     SRAIKAFYDETVAQGVASQVTTFTMSDFNRTLNPAGGGSSVGTDHGWGGYYFVVGGAV
                     AGGNFYGRPTSNGTFVPTLVMGTGDDADTRGRFIPTVSVEQYAATLARWYGLADADIP
                     LVFPNITNFPVSNLGFMM"
BASE COUNT          826 a         1034 c         1044 g          775 t
ORIGIN      
        1 cttcagcaat aacaacctta gcggaaactg gtacgcgcaa gtcgtagacc gtcagacggg
       61 cggctcgctt cccgctccga atacgactaa tctcaagaac tttagaggaa actggtgggg
      121 cactgccagc cccgtcgtga ccaccgccaa cagcgcggaa cccggctacg cggcacaaat
      181 cccggtcgcc tacggcggaa cggcgactgc tcccggcgga caaccggata ttgccggagc
      241 ggcttcggct aatattaaat atatgccgtt gctgaccaac ggcggcgata ccaatatcga
      301 aacaacgccg ggacgcggaa ctttcgggtt tcagggcgcg cgcagcgtag tcagcgtaaa
      361 gccttcgatg tcgaacggct ggtcggcggt cagccagaga accgcttcgg gcagctttgt
      421 caacggtccc ggaatgccgc ctttaggttc gggcagttat cgaatgatgc tcggagcggg
      481 aaattcaggt cctgatttgc cgcagggcgg cgcgggacag ggcggcaaga cctggctgac
      541 cacgcagcaa ttcgataata ctttgctgtc cggcattagc gtgctgggct acagcactta
      601 tgttgccgcg tcgccttctt cggctggtaa tatcattacg ccgaccttgc agcttcagat
      661 tgatttggac ggtaacggga cgcgggactc gacaatgatt ttcgagcctt attattcaac
      721 cgctgccggc ggcggcacgc aatctaatgt cgctctcggt caatggcaaa cctgggatgc
      781 cagagccggt aaatggtggt tcaacaatgc caccgtcttc ggctgcgggc aatgcgcttt
      841 cccgactttt gacgccataa tcgccgctta cccgaatgcc aaaatcgtga cctggtatgc
      901 gctggcggac ggttacggaa cgcagctggt ggcgggacaa aattccgcgg gctcgccgtg
      961 ggctaacttc gacgggaata tcgacgcttt cactatcacg gtcaatgccc cgaacacgac
     1021 cttcgacttt gaacctctgg cgccgacggc ggcgagcgtc tcgattgcgg gacaggtttt
     1081 gacggcgacc ggacagagca tcccgaacgc caaaatcttt ctgaccgaca atcagggcgt
     1141 caagcgcgtc gccgtttcaa acgccttcgg tcattacggc ttcgaaggca ttacggtcgg
     1201 cgaaacttat attttaagcg tcgtctcgaa acgttatact ttcacgccgc gggctgtgga
     1261 ggtaaacgaa gaactgactg ctttgaattt gattgccgaa ctctaaaaag ctcgacaaaa
     1321 aaacttttgg ggcgaagatt acggtcttcg cccttttttt tattatgccg gtcgttcagg
     1381 attcaggtga atccgaagtt aaaatctcct gcacgcgccc gacctgcccg tccgtcaggc
     1441 gcacctttat cccgtgcggg tgaaaagccg agttagtcaa taagtccttc accgttccga
     1501 cggtttgcct gcccgttcgc tggtcttgtt tcagaacaat cgccacgcgc aaaccggggc
     1561 gcacgtcttt tcgctgtttt ccatccattt ttacattttc gtttatttcg ggaacaaatt
     1621 ccagattgcg gtgtctaatt gcgtgacgag tagaatgttt tcgccttgac ccgcgcgatg
     1681 gcaccggcag caagaggagg aaaattgaat gttggataat ttagtcgaat cgaaaagcaa
     1741 cgccggagaa acgggacgcc gagccggatt tttggtaacc acgcttttta cggtcgtgtc
     1801 gctgtttctg atgtcactga cctacagtct tttcgccaaa gacctcggaa cggacgacaa
     1861 cggcttggag ctttcaactc tggtcgcgcc cgttcccgaa gccgaaacgc cgccgcagcc
     1921 ggaacttcag aaacaacccg aaaggcgcgc agcctcgacg cagtcaaata ttgtgatgag
     1981 aaaagaggct tttgcgccga ttgaacaaaa taccaagccg cccgccgata cgaacgggca
     2041 aaaaggcgtc caaagctggg taaagggagc gaaactgggt tcggctgatt taactgcgtc
     2101 gggtcaaccc aattcgccgg cgcgcagcaa taacgagccg ggcgaaggaa taaacgggaa
     2161 taatgtctcg acggaaaaag aagtcgagcc gccgccaatg ctgacgaaac cgtcgccgaa
     2221 accgccggtc gagcagccga aaaaagaaaa gccgccggtg gtcacgggcg gcgtcgtcaa
     2281 cggcaaagcc atcaatttag tgaagccgca attttcggca gcggcaaaag cgataagggc
     2341 gagcggcgcg gttaacgtgc aggtcttaat cgacgaaaaa ggcaatgtgg tttccgcctc
     2401 ggcggtctcc gatcatcgac tgcttcgcca atcggcggag tctgcggcgc gcgccagcaa
     2461 atttacgccg acgtttttaa ccggtcaacc cgtcaaggtt accggagtca tcgtgtataa
     2521 cttcgcgctt caataactcg aatttgttct ttacttcaaa acaaaagggc tgtttgttta
     2581 taaacaaaca gcctttttcg attatttttc agcccggcgt ttacatcata aaaccgagat
     2641 tggagacggg gaagttggtg atattcggaa agacgagcgg aatgtcggcg tccgccaagc
     2701 cgtaccagcg cgccagcgtc gccgcgtatt gctcgaccga aaccgtcgga ataaagcgcc
     2761 cgcgcgtgtc cgcgtcatct cccgtgccca tcacgagtgt cggaacaaaa gtgccgtttg
     2821 aggtcggtct gccgtagaaa ttgccgcccg cgaccgcgcc gccgacgacg aaataatatc
     2881 cgccccagcc gtggtcggtg ccgacgctgc tgccgccgcc cgccggattg agcgtgcggt
     2941 taaaatccga catcgtaaag gtcgtgacct gcgaggcgac gccctgcgcc accgtttcgt
     3001 cgtaaaaagc ctttatcgcg cggctcacct gcgtcagcaa attgtcgtgg tttcccaatt
     3061 ggttgctgtg atggtcgaaa ccgccaatct ggacaaagaa aatctggcgg ctcattccga
     3121 gcagcgttcg gagctttatc agcttggcga cctgacgcag ctgattgccc agagaggtgg
     3181 ctggaaaagt aaccgttaaa gtcgggtcga cgcttaactg ctggctgacg ttgacggctt
     3241 gctgcgtaat ggcgctcgcc tgttgaacca gcgtgtaatt caaatcgcgc gtccgggcgc
     3301 tgttcatcgc cgccagccgc gccagctcgt ccgtcgcagt gccgaagccg tttaaggtca
     3361 aaacctgatt gagcggcgtc ggagccgcgc cgacaatcag cggtttggtg ctcgcgccgt
     3421 tgttaaaaac ggacgcgccc gcaatcgagg taatcatcgg aacggctccg cccggattca
     3481 gggtgctcgt gcggtcggcg gttctgccgc cccagccggt cgtcgattta aagctcgaaa
     3541 tccccgtctt gaactgctca gcctggtcgg gatgcgaaaa aagctggtag ggacgcggcg
     3601 cgcccgcctg atacgtcgct cgcgtcaacg gctgaacgag acagcccgcg ttgcagacga
     3661 cagccatctt gccctgctg
//
LOCUS       DASWNE010001051         1834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_175285, whole genome shotgun sequence.
ACCESSION   DASWNE010001051 DASWNE010000000
VERSION     DASWNE010001051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175285"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..>1834
                     /locus_tag="VGB68_20045"
     CDS             220..>1834
                     /locus_tag="VGB68_20045"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEY0051601.1"
                     /translation="MQFKAKRILIGSLRAALLIFLSFLFCAETRAEKIPFRSYTTAEG
                     LAHDRVNRIIRDSRGFLWFCTSEGLSRFDGYEFKNYRQDEGLPHRGVMDFLETRDGNL
                     WLATGDGIVLFNPAGAAQNSTGGQEKPDAPKMFRVFRDKDLKADIQSWAAIDLLEDSR
                     GTIWAATEYGLYRLDSAEGDWRLQRVETENWGPLSTDFIQLLEDRTGALWIGAGKGLY
                     RILPDGSVQKIDDRLRVESLLEDRAGRIWVGSGGGESRGLYQFVLAENNQPRLARAFD
                     SRDGLTDDHWMNELLETTDGRIFVAVGDGLNEYTPSSDQNAPQFRTLLNEGLVSLGED
                     TSGNLWLGTVSSGALRLARRGFVAFDEADGLKSRLTASILRGANDELYVLAGDFHRFD
                     GNRFVGVAPLGIVPTNWSTNQISFQDSAGEWWITGGVGLQRYPPVKKLEDLARTPHKK
                     IYTTADGLFTNSVFQLFEDSRGDIWISTIGHESDSVQRWERATDTIHRYTTADGLPFQ
                     NCPTAIGEDSAGNIWLGFYSGGLVRFRQGK"
BASE COUNT          413 a          485 c          531 g          405 t
ORIGIN      
        1 ccgctcttac aaagctttag ccagagcggt tgaattgttc gtttattttt ataatgagcg
       61 acaactcaga aaaagacgct tgccgaagta tccggcttat ctggctgaaa atctgccaac
      121 gcttatttag tcactcccaa acagtaaatg gtattgaaaa ataaattaga aaaatgcaca
      181 atacgggcag caagcttcca gcggcgtttc atcccggcaa tgcaatttaa agcaaaaaga
      241 attttaatcg gctcactgcg cgccgctctg ctgatttttc tttcgtttct tttttgtgcg
      301 gaaacgcgag ccgaaaaaat acctttccga agctacacga ccgccgaagg cttggcgcac
      361 gaccgcgtca atcgcatcat ccgcgattcg cgcggttttt tgtggttctg cacctccgag
      421 ggcttgtcca gatttgacgg ttacgagttt aaaaattacc ggcaggacga aggtttgccg
      481 catcgcggcg tgatggattt tctggagacg cgcgacggga atctctggct ggcgaccggc
      541 gacggaatcg tgcttttcaa tccggcgggc gctgctcaaa attcaaccgg cgggcaggag
      601 aaaccggacg ctccgaagat gtttcgcgtt ttccgcgaca aagatttgaa agccgatata
      661 cagtcgtggg cggcgattga tttgctggaa gacagccgcg ggacgatttg ggcggcgacc
      721 gaatatggac tttaccggct cgattcggcg gaaggcgatt ggcgattgca acgcgtcgag
      781 acggaaaact ggggacctct ttcgaccgat ttcatacagc tgctcgaaga ccggacgggc
      841 gcgctgtgga tcggcgccgg aaaaggactc taccgtattt tgccggacgg aagcgtgcag
      901 aaaattgacg accgcttgcg cgtcgaatcg ctgctcgaag accgtgcggg gcgcatctgg
      961 gtcggttccg gcggcggcga aagccgcgga ctttatcaat tcgtcctcgc ggaaaataat
     1021 cagccgcggc tggcgcgcgc attcgacagc cgcgacggcc tgacggacga ccactggatg
     1081 aacgagctgc tggaaaccac ggacggcaga atattcgtcg ccgtcggcga cggcttgaac
     1141 gaatacactc cttcatccga ccaaaacgcg ccgcagtttc gcactttgtt aaacgaagga
     1201 ttggtttcgc tgggcgagga cacgagcggc aatctctggc tcggaacggt cagcagcggc
     1261 gcgctgcggc tggcgcgccg cggttttgtt gcctttgacg aagccgatgg tttaaagagc
     1321 cggctgaccg cttcgatttt gcgcggcgca aatgacgagc tttatgttct cgccggagat
     1381 tttcatcgct ttgacggaaa tcgtttcgtc ggcgtcgcgc cgctcggcat agttccgact
     1441 aattggagca ccaatcaaat ttctttccag gacagcgcgg gcgagtggtg gattaccggc
     1501 ggcgtcggct tgcagcgtta cccgccggtc aaaaaactgg aagacctcgc gcgcacgccg
     1561 cacaaaaaga tttatacgac cgccgacggt ttatttacca actcggtttt tcagcttttc
     1621 gaagattcgc gcggcgacat atggattagc accatcggac acgaatccga ctcggtgcag
     1681 cgctgggaac gcgcgaccga tacgattcat cgctacacga ccgccgacgg cttgcccttt
     1741 cagaactgtc cgaccgccat cggagaagat tcggcgggaa acatctggct gggattttac
     1801 agcggcgggc tggttcgctt caggcagggt aaat
//
LOCUS       DASWNE010001052         2742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_247171, whole genome shotgun sequence.
ACCESSION   DASWNE010001052 DASWNE010000000
VERSION     DASWNE010001052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247171"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1266)
                     /locus_tag="VGB68_20050"
     CDS             complement(<1..1266)
                     /locus_tag="VGB68_20050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEY0051602.1"
                     /translation="MIKEQIKNLITEKTARVGVIGLGYVGLPLIVEFALKGFEGIGFE
                     VDAKKAEAINKGESYIVDVTSENVKKCVDAGKLKATTDFSELEKCDVIIICVPTPLRK
                     TKDPDMSYILAAGEQIQKYMRRGQLIILESTTYPGTTDEVLQPMFEEKGFKLDEDFLL
                     AFSPERVDPGNPQFQTHNITKVVGGVGKDSTEVSAQLYAQIVDNVHAVSSARVAEACK
                     LWENTFRAINIGMANEMAKVCNALGIDTWEVVRAAATKPFGFMPFYPGPGIGGHCIPL
                     DPHYLSWKARQHGFDSRFITLAEEINSSMPKYVAGLVRDALNEQEKSVKGAKILILGV
                     AYKKDIDDMRESPALSVIDLLRARGAEVFYHDPFVPEVHFDHAYTIGAGDPLYNSDLT
                     DDLIKSSDCVVICTEHSEVDYKRVCELASV"
     gene            1621..>2742
                     /locus_tag="VGB68_20055"
     CDS             1621..>2742
                     /locus_tag="VGB68_20055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051603.1"
                     /translation="MNIQTTSTNPIVKAVVEGTAPRPARLAASRGILPLPQTDLLEIL
                     VALAQNGDEELSANARETLKNQDKQRFGEVVSAPQISANVLAYFAGEENLPFDAYEAI
                     ITNLNTPDAAIIKFARNTKEGSLLELVTLNQQRLIRTPQIIEAIIANPYRTAEAERRA
                     QETKREFFQKERGAQQIANELRAQGLEAAAEFIEQAEFAGNLEESNLTVDDALLIAKH
                     IEISDSEIDDSWLSSDLIEEFYEETEEQRQAILGKILGELRMENDEISGERVSMLNRI
                     LRMSMKDRVKLAMKGDREARNILIRDPNRIVAQAVMQNPRLTEQEVEKIASMRTVPED
                     VLRQIAASRQWSRIYTIVHNLARNPRTPLSNVMSILTRLQ"
BASE COUNT          676 a          725 c          703 g          638 t
ORIGIN      
        1 aactgaagca agctcgcaaa cgcgcttgta atcgacttcc gagtgctcgg tgcagatgac
       61 gacgcaatcg gacgatttga ttaaatcgtc ggtcaagtcg gaattgtaga gcgggtcgcc
      121 cgcgccgatg gtgtaagcgt ggtcgaaatg aacttcgggc acaaacgggt cgtgataaaa
      181 aacctccgcg ccgcgcgcgc gcaataaatc aatcaccgac agcgcgggcg attcgcgcat
      241 atcgtcgatg tcttttttat aagcgacgcc caaaattaaa atcttcgcgc ctttgaccga
      301 tttttcctgc tcgttcagag cgtcgcgcac cagtccggcg acgtatttcg gcatcgagga
      361 attaatttct tcggcgagcg tgataaatct ggaatcgaaa ccgtgctggc gcgctttcca
      421 cgaaagataa tgcgggtcga gggggatgca gtgtccgccg attccgggtc ccggataaaa
      481 cggcataaag ccgaacggct tggtcgccgc cgcgcggacg acttcccagg tgtcgatgcc
      541 gagcgcgttg cagactttcg ccatctcgtt cgccatcccg atattgatgg cgcgaaaagt
      601 gttttcccac aatttgcagg cttcggcgac gcgcgcgctg gagacggcgt ggacgttatc
      661 gacgatttgc gcatacagct gcgccgaaac ttcggtcgaa tctttgccga cgccgccgac
      721 gacttttgta atattgtgcg tctgaaactg cggattgccc gggtcgacgc gctcgggcga
      781 aaaggcgagc agaaaatcct cgtccagctt aaagcctttt tcctcaaaca tcggctggag
      841 aacttcgtcg gtcgttcccg ggtaagtggt cgattcgaga ataatcaact gaccgcgccg
      901 catatatttc tgaatctgtt cgcccgccgc cagaatgtaa gacatatccg ggtctttcgt
      961 tttcctgagc ggcgtcggga cgcagatgat aatcacgtcg catttttcaa gctccgaaaa
     1021 gtcggtcgtc gcttttaatt tgcccgcatc gacgcatttt ttgacatttt cgctcgtcac
     1081 atcgacgata taagattcgc ccttgttaat cgcctcggct tttttcgcat cgacttcaaa
     1141 accgatgcct tcgaagcctt tcaaagcaaa ctcgacaatc aagggcaagc cgacatagcc
     1201 gagcccgata acaccgacgc gcgcagtttt ttccgtaata agatttttaa tctgttcttt
     1261 tatcattttt atattaaaaa ttagagaaaa atggatttta catagaaggc tgattagtca
     1321 tattgaggcg caagaaacgc tccactcttt tagactaagt gatgccagtt tagctgtcaa
     1381 gaaccttttt atagtggtaa acggtaaacg gcagacggta agcggcaagt ccgttatttc
     1441 gactttgagt ttgaaataaa cattcgatac tttgcaattg cagcttggcg tttagcgttt
     1501 agcgtttagc gcttaccgct ttaaatctat ccattttcaa tcagacaatt atgttataat
     1561 tattttcggg tgcaaagccc gaatgctcca aaactttcca gacccttctt tccgactttt
     1621 atgaatatcc aaacaacatc cacgaatccg attgtcaaag cagtcgtcga aggcactgcg
     1681 ccgcgtccgg cgcgcctcgc cgcttcgcgc gggattctgc ctctgccgca gacggatttg
     1741 ctcgaaattc tcgtcgcgct ggcgcaaaac ggcgacgagg aattgtccgc caacgcccgc
     1801 gaaacgttaa aaaatcagga taaacagcgc ttcggcgagg tcgtttccgc gccgcagatt
     1861 tcggcaaacg tgctggcgta tttcgcgggc gaggaaaatc ttccttttga cgcttacgaa
     1921 gcgattatca cgaatttaaa cacgcccgat gcggcgatta taaaatttgc gcgcaatact
     1981 aaagaaggaa gccttctgga gcttgtcacg ctgaatcagc agcgcctgat tcgcacgccg
     2041 caaatcatcg aagcgattat cgccaacccg tatcgcacgg cggaagccga gcggcgcgcg
     2101 caggaaacca agcgcgaatt tttccagaaa gaacgcggcg cgcagcagat tgccaacgag
     2161 cttcgcgcgc aggggctgga ggcggcggcg gaatttatcg agcaagccga gtttgccgga
     2221 aatctggaag aatcgaacct gacggtcgac gacgctcttt taatcgccaa acacatcgag
     2281 atttccgatt cggaaatcga cgattcgtgg ctttccagcg atttaatcga agaattttac
     2341 gaggaaaccg aagagcagcg acaggctatc ttaggcaaaa ttctcggcga attgcggatg
     2401 gaaaacgacg agatttccgg cgagcgcgtt tcgatgctga accgcattct gcggatgagc
     2461 atgaaagacc gcgtcaaatt ggcgatgaaa ggcgaccgcg aagcccgcaa cattctcatc
     2521 cgcgacccga accgcatcgt cgctcaagcc gtcatgcaga atccgcgcct gaccgagcag
     2581 gaagtggaaa aaatcgcttc gatgcgcacc gtgcccgaag acgttctgcg gcaaatcgcc
     2641 gccagccgtc aatggtcgag aatctacacg attgttcaca atctggcgcg caacccgcgc
     2701 acgccgcttt ccaacgtgat gagcatttta acgcgcctgc ag
//
LOCUS       DASWNE010001053         5214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_175391, whole genome shotgun sequence.
ACCESSION   DASWNE010001053 DASWNE010000000
VERSION     DASWNE010001053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5214
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175391"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..1151
                     /locus_tag="VGB68_20060"
     CDS             111..1151
                     /locus_tag="VGB68_20060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY0051604.1"
                     /translation="MKRFYKNFVSRLMFFAALVALFPALGCLPSAPETKHGSRTITVY
                     GFSIMKEALEKEIYPAFAAKWKREHGEDVQFVSSFAGSETVTNQILQGAPADLAILSI
                     ERDVERLSSGGAVTQNWKSAPHNSIANKTPFIILVRKGNPLGIRDFPDLAKPGVKIIH
                     PDPVSSGGAQWSILSIYGSELKKTERQTGSMNTAQALETLQAVWKNVISTPESARAAR
                     TQFETGYGDVLITYELEGLMMKQAGAPVDMVVPQSTIFSEHPAVVIDRNVTAEERAVV
                     QGFLSYLWSDEAQQAFVKYHFRSITDESYNDANAEFAKIELPFTVEMFGGWTRAYPEI
                     IESVFRDKVQKK"
     gene            1227..2063
                     /locus_tag="VGB68_20065"
     CDS             1227..2063
                     /locus_tag="VGB68_20065"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY0051605.1"
                     /translation="MSANISNVRHVRGFDIVKPLSITAMIAIMLPLILLPLASIFIFG
                     TSKGLVSFWHALTASEAIFALQLSIVTSFWATVFNVIFGLFAAYVLTKYNFWGRNALI
                     VIISLPTAIPTAVAGFALLLLWGPYGTLGRLLEPQGVQIMFTASAVILANIFVTFPLA
                     FGVIKPVYETMSQSLEEASATMGATRWQTFRHVTLPSLRGAIVTGALLTFARAVGEFG
                     STILVSGNLASRTQTAPLYIFAKFNEGQVEAANAIAIVLAIFSFLIFSILFFARDWLE
                     AE"
     gene            2241..3377
                     /locus_tag="VGB68_20070"
     CDS             2241..3377
                     /locus_tag="VGB68_20070"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY0051606.1"
                     /translation="MIESKANKIIAVSVRSVSKRFGAATVLEDINFDVDEGETVVLLG
                     ASGSGKTTILRVIAGLEKPDSGHIILHGRDVTEMPARERGVGVIFQSYALFPKMSVEQ
                     NIGYGLHLRGRPKSEIKEAVNNLIELIGLEEHRRKRPSQLSGGQQQRVAIARALAYKP
                     EVLLFDEPFGALDAQIRARLRREIRALLKKINVPSIFITHDQEEALELGDRIAVLNAG
                     RLEQIGTPYEVYNTPATEYVATFVGAANLLLGVIRGQNFEVEDVTLAIEDQTNFRDGQ
                     SVKLVFRPEDVFLRKPENLTQHYQKLTDAAVEEINFVGAFERVVVRLDLSTRQPIIIT
                     RPKTETTAFPLRVGQKVTVGLVRFRILPNFTLASERSSRTVDAG"
     gene            complement(3464..4231)
                     /locus_tag="VGB68_20075"
     CDS             complement(3464..4231)
                     /locus_tag="VGB68_20075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051607.1"
                     /translation="MKPTFHVLLLVFLAFAFQIQAQKKRRLITPVKNPVAENQIRRES
                     ANTAIVVDERLAVLRASPSLYAMPVQRMRRGRKILISGVKDGDGVTFYRVNLSSESAG
                     WVQAEAVVGKFRRGDDERLARLVQSSEGFDQIERATIFLENFPKSPLRPAMLLLFGDL
                     VEENAAMISREATRRLSRREMTATGAPIHSFYLNYASLDRYRRLKVGFVFNPDTKILH
                     YDGAAWKEILTRFPKSSEAAEAQKRLDSLKEKLGRKQ"
     gene            complement(4265..4777)
                     /gene="ruvC"
                     /locus_tag="VGB68_20080"
     CDS             complement(4265..4777)
                     /gene="ruvC"
                     /locus_tag="VGB68_20080"
                     /inference="COORDINATES: protein motif:HMM:NF000711.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEY0051608.1"
                     /translation="MKVLGIDPGSETLGWGVVEGVGLKYNLVDFGTVKSSPREKFSKR
                     LLKAYTGVSEVIQKFQPDVISIEEAFYAVNVNVALKLGQVRGAMLLLGEISGLEIAEY
                     SPRLVKQTVVGYGNAEKHQVQEMVRVLLRLQKAPEPHDAADALAIAICHFHHAGVQEK
                     IKNAKLKLRR"
BASE COUNT         1218 a         1398 c         1320 g         1278 t
ORIGIN      
        1 tactttatgc ggcaaaacga cggattctct cgccccggcg atttgaacgt cgtcggcacg
       61 agctggaaag tgcgcctgta aatcggggcg cgggctggga gatttgaaat atgaaacgtt
      121 tctataaaaa ttttgtttcg cgtttaatgt ttttcgcggc gctcgtcgcg ctttttccgg
      181 cgctcggctg cctgccgagc gcgccggaga cgaaacacgg cagccggacg attaccgtct
      241 acggcttttc gattatgaaa gaagcgctgg aaaaagaaat ctatccggcg tttgccgcca
      301 agtggaaacg cgagcacggc gaggacgtcc aattcgtatc gtctttcgcc ggttcggaaa
      361 ccgtgaccaa tcaaatcctg caaggcgcgc ccgccgacct tgccattctc tccatcgagc
      421 gcgacgtgga aagactttcg agcggcggcg cggtcacgca aaactggaaa tccgcgccgc
      481 acaacagcat cgccaacaaa acgcctttca ttattctcgt ccgcaaaggc aatccgctcg
      541 gaattcgtga ttttcccgat ttggcaaaac cgggcgtaaa aattattcat cccgacccgg
      601 tcagttccgg cggcgcgcaa tggtcgattc tttcgattta cggctcggag ctgaaaaaaa
      661 ccgagcgaca gaccggcagt atgaacacgg cgcaggcgct cgaaaccttg caggcagtct
      721 ggaaaaacgt catttccacg cccgaatcag cccgcgcggc gcggacgcag tttgaaaccg
      781 gttacggcga cgtgctgatt acatacgagc tggaaggctt gatgatgaaa caggcgggcg
      841 cgcccgtcga tatggtcgtg ccgcaatcaa ccattttcag cgagcatccg gcagtcgtca
      901 tcgaccgcaa cgtaaccgcc gaagaacgag ccgtcgtgca gggctttttg tcttatctgt
      961 ggagcgacga ggcgcagcag gcgtttgtga aatatcattt tcgctcgatt accgacgaat
     1021 cttataacga cgcgaacgcc gaatttgcca aaatcgagct gccgtttaca gtcgagatgt
     1081 tcggcggctg gacgcgcgct tatcccgaaa taatcgaatc cgtctttcgt gataaagtgc
     1141 agaaaaagtg aataactgat aactgataac tgataatttt tttctttcta ctatcagtta
     1201 tcagttatca actatcagtt aaaaccatga gcgcgaatat ctccaacgtc cgtcatgtgc
     1261 gcggtttcga cattgtcaaa ccgttgagca taaccgcgat gatagcgatt atgctgccgc
     1321 ttatcctgct tccgctggct tcgattttca ttttcgggac aagcaaaggc ttggtcagct
     1381 tctggcacgc tttgaccgcg tccgaagcga ttttcgcgct tcagctttcg attgtgacga
     1441 gcttctgggc gacggttttc aacgttattt tcggtttgtt cgccgcttac gttctaacga
     1501 aatacaattt ctggggacgc aacgccttaa tcgttatcat ttcgctgccg acggcgattc
     1561 cgacggctgt cgccggattc gccctgcttc tgctctgggg tccgtacgga acgctcggac
     1621 gcctgctcga accgcagggc gttcagataa tgtttacggc ttcggcggtc attctggcaa
     1681 atattttcgt cacgtttccg ctcgccttcg gcgtcatcaa gccggtttac gagacgatga
     1741 gtcaatcgct cgaagaagcc tcggcgacga tgggcgcgac gcgctggcag actttccggc
     1801 acgtgacgct gccgagtttg cgcggcgcga ttgtcaccgg cgcgctgctg acgtttgcgc
     1861 gcgcggtcgg cgaattcggc tcgaccattc tggtctcggg caatctggca agccggacgc
     1921 aaaccgcgcc gctttatatt ttcgccaagt tcaacgaagg tcaggtcgag gcggcaaacg
     1981 cgattgcgat tgtgctggcg attttttctt ttttgatttt cagtatttta ttcttcgcgc
     2041 gcgactggct ggaagcagaa taaaaagatt caagattcag gattcaagat tcaagattca
     2101 ggatttagaa ttcaggattt aagatgtgcc attattgaaa gacaatcctg aatcccgaac
     2161 cttaaatctt gaatcttgaa tcctgaatct tgaatcttga atcctgaatc ctgaatcctg
     2221 aatcttgaat cctgaattta atgatcgaat caaaggcaaa caaaataatc gccgtcagcg
     2281 tccgcagcgt cagcaaaaga ttcggcgcgg cgaccgttct cgaagacatt aatttcgatg
     2341 tcgatgaggg cgagacggtc gtgctgctcg gcgcttcggg cagcggcaaa acgacgattc
     2401 tgcgcgtcat tgccggattg gaaaaacccg attccggaca cattattctg cacggcagag
     2461 acgtgaccga aatgcccgcc cgcgagcgcg gcgtcggcgt tatctttcaa tcctacgccc
     2521 tttttccgaa aatgagcgtc gagcagaaca tcggttacgg cttgcacctg cgcggtcgtc
     2581 cgaaaagcga aattaaggaa gccgtcaaca atctaatcga gcttatcggg cttgaagaac
     2641 atcgccgcaa acgtccttcg cagctttccg gcgggcagca gcagcgcgtc gccatcgccc
     2701 gcgcgctcgc ctacaaaccg gaagttttgc tgtttgacga gccgttcggc gcgctcgacg
     2761 cgcagattcg cgcgcgtctg agacgcgaaa ttcgcgcgct tttgaaaaaa atcaacgtgc
     2821 cttcgatttt tatcacgcac gaccaggaag aagctctgga gctcggcgac cgcatcgccg
     2881 tcctgaacgc cggtcggctc gaacaaatcg gaacgccgta tgaagtttac aacacgcccg
     2941 cgaccgaata cgtcgcgacc tttgtcggcg cggcgaatct tctgcttggc gtcattcgcg
     3001 ggcagaattt cgaagtcgaa gacgtcacgc tcgcaatcga agaccagacg aattttcgag
     3061 acgggcaatc ggtcaagctg gtttttcgtc ccgaagacgt ttttctgcga aaaccggaaa
     3121 atctcacgca gcattatcaa aaattaacgg acgcggcggt cgaggaaata aatttcgtcg
     3181 gcgcgtttga aagagtcgtc gtcaggctgg atttatcgac ccgccagccg attatcatca
     3241 ccagaccgaa aaccgaaacg accgcgtttc cgcttcgcgt cgggcaaaaa gtgacggtcg
     3301 gcttggttcg tttccgcatt ttgccgaatt tcacgctcgc ttccgagcgt tccagccgaa
     3361 cggttgatgc aggctgaaat tttaaactat gaacgcgcaa tgatgaacgc gtaattgcag
     3421 ttttcgcctt catcgctaaa cattgcgcgt tcaacttttt ttactactgc ttccgcccga
     3481 gtttctcttt cagcgaatcg agccgttttt gcgcttcggc ggcttcggac gattttggaa
     3541 atctggttaa aatttctttc cacgccgcgc cgtcgtaatg caggattttc gtgtccggat
     3601 tgaaaacgaa tccgactttc aggcgtcgat agcggtcgag gctcgcgtaa tttaaataaa
     3661 aactgtggat cggcgcgccc gtcgccgtca tctcgcgccg gctcaaacgg cgcgtcgcct
     3721 cgcgtgaaat catcgccgcg ttttcctcga ccaaatcgcc gaacaacaaa agcatcgccg
     3781 gacgcagcgg agatttcgga aaattttcga gaaaaatcgt cgcccgctcg atttggtcaa
     3841 agccttcgga cgattgcacc agacgcgcca gacgctcgtc gtcgccgcgt cgaaattttc
     3901 cgaccaccgc ttccgcctgc acccagccgg cgctttccga cgaaagattg acgcgataaa
     3961 acgtcacgcc gtcgccgtct ttgacgcctg aaatcaaaat ctttcgcccg cgccgcattc
     4021 gctgcacggg catcgcgtaa aggctcggcg acgcgcgcaa aacggcgagt ctttcatcga
     4081 cgacaatcgc cgtgtttgcg ctttcgcgcc ggatttggtt ttcagcgacc ggatttttga
     4141 cgggagttat caagcggcgt ttcttttgcg cttgaatctg aaaggcgaaa gcgagaaaaa
     4201 cgagcagaag aacgtgaaac gtgggtttca tatgggcttc tccctttgaa aaacgcaaaa
     4261 tcgtttaacg cctgagcttc aattttgcat ttttgatttt ttcctgcacg ccggcgtgat
     4321 gaaaatgaca gatggcaatc gccagcgcgt cggcggcgtc atgcggctcg ggcgctttct
     4381 gaagacgcaa aagcacgcga accatttcct gaacctggtg tttttcggcg tttccgtagc
     4441 cgaccaccgt ttgtttgaca aggcgcgggc tgtattcggc gatttccaaa ccggaaattt
     4501 cacccaaaag cagcattgcg ccgcgaactt gtccgagttt caaagcgaca ttgacgttga
     4561 ccgcgtagaa agcctcttct atggaaataa cgtcgggctg aaatttttga atcacttccg
     4621 aaacgcccgt gtaagccttc aaaagacgct tggaaaactt ttcgcgcggg ctggatttga
     4681 ccgtgccgaa atcgaccaga ttgtatttca atccgacgcc ttcgaccacg ccccagccga
     4741 gcgtttcgct tcccgggtca atgcctaaaa ctttcatttc gtaatacgta atacgcagca
     4801 cgtaatacgc ccgtaaaagt caatcagaaa gctccgagct tcctgattga cgggaaagcc
     4861 gtcgtttgat tttatcaaca ttataattta actccaaata ttttctctcg tcaatacatt
     4921 ttctgccttt tgtcgataaa atctgacagt cgactttaaa agaaaagccg cgcgcaatcc
     4981 gcaaaaaccc gtatcctctt aatttactgt aatataatgg ttttcaacta ttcttgccgg
     5041 agcagaagag attgtcgggc gcatttaatt cgccgagtaa aaatttcttc cttcgacgtt
     5101 tttcacgatg cctgataaac atttcgtgtt aatacgcgcg gctctcgcca aactttaaac
     5161 cgtcgaaaat ctcacaggct tttccattcg gaagttttct cggaaatttt cgcg
//
LOCUS       DASWNE010001054         4311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_140519, whole genome shotgun sequence.
ACCESSION   DASWNE010001054 DASWNE010000000
VERSION     DASWNE010001054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140519"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            397..1665
                     /gene="preA"
                     /locus_tag="VGB68_20085"
     CDS             397..1665
                     /gene="preA"
                     /locus_tag="VGB68_20085"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006183.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HEY0051609.1"
                     /translation="MADLSVNFAGIKSPNPFWLASAPPTNMGSMVERAFDAGWGGAVW
                     KTLGEPIVNVCSRLASLDNGSNRMIGLNNIELITDRPLEVNLKEIYECKKKYPNHAVI
                     VSLMVESKREAWHEIVKRAQDTGADGFELNFGCPHGMSERGMGAAMGQVPEYTCMVTE
                     WVKEVSELPVIVKLTPNVTNIIPPGRAATKGGADALSLINTINSVMGVDIDTMIPHPN
                     VNGMAAHGGYCGTAVKPIALNMVSELARDAEINIPISGIGGINTWRDAVEFLLLGASN
                     VQVCTAVMHYGYRIVEDMIDGLNNYLDEKGFASVGDIVGKSVSRVTDWGNLDLRYKTV
                     ARINNENCIRCNLCHIACEDGAHQCIDFTEVGGLRFPVVDEPECVGCNLCYLVCPSPG
                     AISMIRLDDGSEPLSWRELSSRQPETMTGD"
     gene            complement(1744..3420)
                     /locus_tag="VGB68_20090"
     CDS             complement(1744..3420)
                     /locus_tag="VGB68_20090"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY0051610.1"
                     /translation="MKNLMKLKATTLLSLFCILLQAPVFVLAQTPKTAPANDINAQIR
                     KEGMENSQAMRTIHFFTDVYGPRLTGSPNLKAAGDWAVRQMKEWGFDKAQLEPWNFGH
                     PGWVNERASGFITAPVQDSLVFEVLAWTPGTNGVVKGDAFVMTLPERPTQEELTNYFN
                     SIRGNIKGKMVLVGTPARIPVGFTAVPKRFEDEALKRRFDPNPSPTPTPAATPAPSPT
                     PTPKPGQLRFEQIIEQLDKFLLDGGAAVRINDARREHGQIRAFDNDTFDVSKVVPTVV
                     MRNEDYGRISRLVADGLAVSLEFDIRNRSIPEGATSYNVIGEIAGTDKKDEVIMLGGH
                     LDSWHAATGATDNAVGCAIMMEAARILKTIGVKPRRTIRVALWSGEEQGLLGSQAYVK
                     EHFGSFEEQKPGYEKFGGYFNVDTGTGRVRGMSVFGPPEGATVLREILAPFADLGAVG
                     AISTKNRALGGSDHTSFNQAGLPGVGVAQDPIEYFTHTWHTNLDTYERIVEDDVKKSA
                     IVIAASVYALAMRNEMLPRFKPAEMPTPPPTPTPTPAPVASPSPATAPRP"
     gene            3819..>4311
                     /locus_tag="VGB68_20095"
     CDS             3819..>4311
                     /locus_tag="VGB68_20095"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY0051611.1"
                     /translation="MKKPEKDLETGEQTADLSKLGSARRKFLGQVSTTTAALLAANLI
                     SEQQAPAFDEVLSDSIVEAAVSDENALTVKLNINGATQTLTLDSRVTLLDALRERLNL
                     TGSKKGCDHGQCGACTVIVDGRRVLSCLTLAAMCEGKEVTTIEGLAKGEALHPMQAAF
                     IKHD"
BASE COUNT          962 a         1117 c         1241 g          991 t
ORIGIN      
        1 gcgtgattat ttaataaacc ggctggatta ttaatattct ttcaacgatt ttaaggacaa
       61 ttttacagca gattcgggtg agaatttcgc cggaatagat gagcttgcaa atttgtttaa
      121 attgtaaaga gctgggatta acgtggcaca tggacgacga agaccgcacg tggtggtctt
      181 gttcggagtg tgattttgaa attgaagaag acgaaagtaa ggaatgttgc tgcgggaaat
      241 gtaattctcc tttgccttcc gtttcccggc tgatgaaagc cggcgaagga ttttaccggt
      301 gcttttcctg cagcgaaaaa acagaccgaa taaaagaaga cattattgat tacgaggctt
      361 ggttaaaaga acccggcaat ccgaatacat aaaattatgg cagatttaag tgtaaatttt
      421 gcgggaatca aatcgccgaa tcctttctgg ctggcgtcag cgccgccgac gaatatgggt
      481 tcgatggtcg agcgggcgtt tgacgcgggc tggggcggcg cggtctggaa gacgctgggc
      541 gagccgatcg tcaatgtctg ctcgcggctg gcgtcgttgg ataacggctc gaaccggatg
      601 atcggcttga acaacatcga gcttattacc gaccgcccgc tggaagtcaa tttgaaggaa
      661 atctacgagt gcaaaaagaa atatccgaat cacgccgtca tcgtttccct gatggtcgaa
      721 tcgaagcgcg aggcgtggca cgaaatcgtc aagcgcgcgc aggacacggg cgcggacggc
      781 tttgaattaa atttcggctg cccgcacggg atgagcgagc gcggaatggg cgcggcgatg
      841 ggacaagtgc ccgaatatac ctgtatggtg acggaatggg taaaggaagt ttccgaattg
      901 cccgtcatcg tcaagctgac gccgaacgtg acgaatatca ttccgcccgg acgagccgcg
      961 acgaaaggcg gcgcggacgc gctttcccta ataaatacga taaattccgt gatgggcgtg
     1021 gacatcgaca cgatgattcc gcatccgaac gtcaacggaa tggcggcgca cggcggttat
     1081 tgcggcacgg cggttaaacc gattgcgctg aatatggtct cggagttggc gcgcgacgcg
     1141 gagataaaca tcccgatttc gggcatcggc ggcatcaata cgtggcgcga cgcggtcgag
     1201 tttctgctgc tcggcgcttc aaacgtccag gtctgcacgg cggtgatgca ttacggctat
     1261 cgcatcgtcg aagatatgat tgacggcttg aataattatc tggacgaaaa aggcttcgct
     1321 tcggtcggcg acattgtcgg aaaatccgtc agccgcgtga ccgattgggg aaatctcgat
     1381 ttgcgttaca agacggtcgc gcgcatcaat aacgaaaact gcattcgctg caatttgtgt
     1441 cacatcgcct gcgaggacgg cgcgcatcaa tgtattgatt ttacggaagt cggcgggctg
     1501 cgcttccccg ttgtggacga gccggaatgc gtcggctgca atctctgtta tctggtctgc
     1561 ccgtcgccgg gcgcgatttc gatgattcgc ctcgacgacg gcagcgagcc gctgagctgg
     1621 cgcgagcttt ccagccgtca acccgaaacg atgacgggcg actgaaaaag ggtaaaggcg
     1681 aaaggcgaaa ggcgaaagaa agggaaattt tatttcttca atctttcgcc tttcgccttt
     1741 acgttacggt cgcggcgcgg ttgccggact cggcgacgcg acgggcgcgg gcgtcggggt
     1801 cggagtcggc ggcggtgtcg gcatttctgc gggcttgaag cgcggcagca tctcatttcg
     1861 catcgccagc gcgtaaacgg aggcggcgat gacgattgcc gattttttca cgtcgtcctc
     1921 gacgattcgc tcgtaagtgt cgagattcgt gtgccacgtg tgcgtgaaat attcgatcgg
     1981 gtcttgcgcg acgccgacgc cgggcagccc ggcttggtta aacgacgtgt ggtctgagcc
     2041 gccgagagcg cgatttttcg tgctgattgc gccgaccgcg cctaaatcgg caaacggggc
     2101 aagaatctcg cgcagaaccg tcgcgccttc cggcggaccg aaaaccgaca ttcctctgac
     2161 tcttcccgtt cccgtatcga cgttgaaata gccgccgaat ttttcgtaac cgggcttctg
     2221 ttcttcaaac gagccgaaat gttccttcac ataagcctgc gagcctaaaa gtccctgttc
     2281 ttcgcccgac cagagcgcga cgcgaatcgt gcggcgcggc ttgacgccga ttgttttcaa
     2341 gattcgcgcc gcttccatca taatcgcgca gccgacggcg ttgtcggtcg cgccggtcgc
     2401 cgcgtgccac gaatcgagat gcccgccgag cataatcact tcgtcttttt tgtcggttcc
     2461 ggcgatttcg ccgatgacgt tgtagctggt cgcgccttcg ggaatcgagc ggttgcgaat
     2521 gtcgaattcg agcgaaaccg ccaatccgtc cgccaccagc cgggaaatgc gaccgtaatc
     2581 ttcgttgcgc atcacgacgg tcgggacgac ctttgaaacg tcgaaggtgt cgttgtcgaa
     2641 agcgcgaatc tgaccgtgct cgcgccgcgc gtcgttgatt ctcacggcag cgccgccgtc
     2701 gagcagaaat ttatcgagct gctcgataat ctgttcgaac cttaactgtc cgggtttcgg
     2761 cgtcggcgtc ggcgagggcg cgggcgtggc agccggcgtc ggggtcggcg aaggattcgg
     2821 gtcaaaacgt cttttcaaag cttcgtcttc gaatcgtttc ggcacggcag tgaacccgac
     2881 cggaatccgc gcgggcgttc cgaccagaac cattttccct ttgatatttc cgcggatgct
     2941 gttgaaataa ttagtcaatt cttcctgcgt cggtcgttcc ggcagcgtca tcacaaaggc
     3001 gtcgcctttg accacgccgt tcgttcccgg cgtccacgcc agcacttcga aaaccagcga
     3061 atcctgaacg ggcgcggtaa taaacccgga ggcgcgctcg ttgacccagc ccggatgacc
     3121 gaaattccag ggctcgagct gcgctttgtc gaaaccccat tccttcattt gtctgaccgc
     3181 ccaatcgccc gccgccttca gattcggcga gccggtcagc cgcggtccgt aaacgtccgt
     3241 aaaaaagtga atcgtccgca tcgcctgcga attttccatt ccttccttgc ggatttgcgc
     3301 gttgatgtca ttggcgggcg cggtcttcgg cgtttgcgcc aaaacgaaga ccggagcttg
     3361 cagcagaata caaaacagcg atagaagcgt cgtcgctttt aatttcatta aatttttcat
     3421 ttttcctctg tcggcagaat tttaatatgg ctttcctgat tgttacgcgt atttcaaaaa
     3481 atagtttgtc atttttgaaa aagtataatc tttcagacgc cgttttgaca gtaaaagtta
     3541 cacggacgac cggcggcgga aaacgatgga cggtaattga aagaaacgag cccggcgctt
     3601 gcgtttaccg cttatcgctt gctgtttacc gcttatccgc gtcgcaagcc cgaaaaacgg
     3661 aaaattaaat gacaagcaaa tttggcgcgt gataaaatat ttccagttct ccaaacatac
     3721 gacttgacgc tttccgtttg cacaattcac gagcggaaaa ttattagaaa agctttgact
     3781 tttccgtttt cggtttattc cggcgcttgg gtaattctat gaaaaaacca gaaaaagatt
     3841 tagaaaccgg cgagcagacc gccgatttat cgaagctcgg ctcggctcga cgcaaatttt
     3901 taggacaggt ttcgacgacg accgccgcct tgcttgccgc taatttaatc agcgagcagc
     3961 aggctccggc gtttgacgaa gttttgagcg attcgattgt cgaagccgcc gtatcggacg
     4021 aaaacgcgct gaccgtcaaa ctgaatatca acggcgcgac gcagacttta acgctcgatt
     4081 cgcgcgtgac tttgctcgac gccctgcgcg agcggctcaa tctgacgggc tcgaaaaaag
     4141 gctgcgacca cggtcaatgc ggcgcttgca cggtgattgt cgacggtcgg cgcgttttgt
     4201 cgtgcctgac gctggcggcg atgtgcgaag gaaaggaagt gacgaccatt gaaggcttgg
     4261 cgaagggcga ggcgctgcac ccgatgcagg cggcgtttat caaacacgac g
//
LOCUS       DASWNE010001055         1948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_387520, whole genome shotgun sequence.
ACCESSION   DASWNE010001055 DASWNE010000000
VERSION     DASWNE010001055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387520"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1012..>1948)
                     /locus_tag="VGB68_20100"
     CDS             complement(1012..>1948)
                     /locus_tag="VGB68_20100"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEY0051612.1"
                     /translation="TAKRVTNFAEVARRFSEMEVVPARYDDEGRKIAGRRSLDAFSRH
                     IEVLTEYFGNLDLQSITYGDLEDFKALRLKTPVKFKSGRQRPRSIRSVNYELTVLKQI
                     FNFAYRRRWLDRSPFDDGRGLINKAAETRRNKTWTRAEERQALALCTCKKYAHMKPAI
                     ICAVDGGFRRGELLHSLWSETSFENGTMIARSYKGKTLHKRTVYMTKRMMAALIEWKI
                     LQKKIKTIKDTSLVIGYDNLQSAWETLREKINRPDLHFHDLRHVFGTRLNQSQKISLR
                     EIQLLLGHSDIRTTQLYLNPSEENLKESIKVLEED"
BASE COUNT          525 a          444 c          450 g          529 t
ORIGIN      
        1 agtttcgatt aattatttcg accagcaaaa tcaactgccg gaaatcaaag caatccgcga
       61 cgatttaaat tcggttcatt ctcaggtttt gcaggacgtt ttaaaacgtc tggacaaaac
      121 ttttaaggct ttttttgagc gtgtaaagcg taaggaaaaa gccggatttc cgcgcttcaa
      181 aagccagaac cgattcgaca gcttttgctt tccgcaaagc ggtttttctt taaccgacaa
      241 aaaacttacc ttatcgaaaa tcggtaaaat taaaatcaag acttctcaaa aagttatcgg
      301 caaagtaaaa acctgtatta taaagcgtga aattgataaa tggtttatcg ttttttcggt
      361 tgaaaccgtt gtcgcgcctt taccgaaaac aaataaacaa ataggtgttg acgccggaat
      421 cgaatcgttt attacgcttt cggacggcac gaaggttgag aatttcaaat attacgaatc
      481 gactcaaagg aaattacgag ttgcgcaaag aagtgttgcg cgaaagaaaa aaggctcaaa
      541 ctcgcgccgg aaagcgattt taaaacttcg caaaattcac gctaaaatca caaaccaacg
      601 cgccgatttt caacataaaa tctcaacttc gcttgtcaat gattttgatt tgattgcgat
      661 tgaaaaacta aatatttcgg gaatgtcgaa aggcatactt tcaaaacaaa ttaatgacgt
      721 cgcttggaat agtttctttc aaaagctgaa atataaagct gaaaatgctg gtaagacgct
      781 ggtagaagta aatccgagtt tcacgagtca ggactgttca tcctgcggta acagagtgaa
      841 aaaagactta tcggttcgca cacatcattg cgcaagatgc ggtttgattc tcgacagaga
      901 cgaaaacgcc gcgataaata tactttcggt agggcttacc gatttgaact taactaaaac
      961 cgctaggtta tgagtgttca aagtatccgc gcttttttcg ggcgtagaat gtcaatcttc
     1021 ttcgagaact ttgattgatt cctttagatt ttcttccgaa ggattcaaat acagctgcgt
     1081 cgttctgatg tcggaatgcc cgagcagcaa ttgaatttcg cgcaaactga ttttttggct
     1141 ttgattcaga cgcgtgccga aaacgtggcg caaatcgtgg aaatgcaaat cgggacgatt
     1201 gattttctcc cgcaacgttt cccaggcgct ttgcaaattg tcgtagccga tgacgagcga
     1261 agtgtccttt atcgttttga tttttttttg cagtattttc cactcgataa gagccgccat
     1321 cattcttttg gtcatataca cggtgcgttt gtgaagcgtt ttccctttgt agctgcgcgc
     1381 aatcatcgtg ccgttttcaa agctcgtttc gctccagagc gaatgcagga gctcgccgcg
     1441 acgaaaaccg ccgtccaccg cgcaaatgat ggcaggcttc atatgcgcgt attttttgca
     1501 cgtgcagagc gcgagcgcct gtcgctcttc cgcgcgcgtc caggtcttat tgcgccgcgt
     1561 ttccgccgct ttgtttatca gcccgcgccc gtcgtcgaac gggctgcggt cgagccaccg
     1621 ccggcgatag gcgaaattga aaatctgctt gagcaccgtc agctcgtagt tgaccgagcg
     1681 gatcgagcgc gggcgctggc gtccgctttt gaatttaacc ggggttttca agcgcagcgc
     1741 tttgaaatct tccaaatcgc cgtaagtgat gctctggagg tcgagattgc cgaaatattc
     1801 ggtcaaaacc tcgatatgac gactgaacgc gtcaagcgag cgccgcccgg caatctttcg
     1861 accttcatcg tcgtagcgag ctggaacgac ttccatctcg ctgaatcggc gcgcgacttc
     1921 agcgaaattc gtgacccgtt tcgccgtg
//
LOCUS       DASWNE010001056         3414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_346986, whole genome shotgun sequence.
ACCESSION   DASWNE010001056 DASWNE010000000
VERSION     DASWNE010001056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346986"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VGB68_20105"
     CDS             <1..333
                     /locus_tag="VGB68_20105"
                     /inference="COORDINATES: protein motif:HMM:NF012968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEY0051613.1"
                     /translation="KSQENIAVAQALADALGADLAASRPICDAEWLPIDRQIGSSGQT
                     VAPKVYIALGISGAIQHIVGMKNAGTIVAINKDGEAPIFDIADYGIVGDLFEAVPVLI
                     EEIKKAKA"
     gene            complement(455..832)
                     /locus_tag="VGB68_20110"
     CDS             complement(455..832)
                     /locus_tag="VGB68_20110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051614.1"
                     /translation="MNSEKKLCPSYTCQEGSILLGIIMRDGRVAFSSDRIIVNEEFVQ
                     IAALGRAPEKRFRFGGQCVQNGCKQWSGNRCGVIDSVIESTGDNRDVSNLPECSIRPE
                     CRWFSQSGAEACAVCPEVVTDLR"
     gene            complement(844..1074)
                     /locus_tag="VGB68_20115"
     CDS             complement(844..1074)
                     /locus_tag="VGB68_20115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051615.1"
                     /translation="MADFIVRIDGLKLSKEAEASINREIQAVVLREVAKIDTGGDFSA
                     RIPNKLRWRGIYLRNKAFDTNFNLEVNEIQQG"
     gene            1394..1627
                     /locus_tag="VGB68_20120"
     CDS             1394..1627
                     /locus_tag="VGB68_20120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051616.1"
                     /translation="MKHLKKIFVAAFLGFLAFAPPGTLIFIGVLLLSFLGKTWFIAGV
                     ATGAVLLAIYLFIYREKLLQADFIKRISRRFKR"
     gene            complement(1712..3097)
                     /locus_tag="VGB68_20125"
     CDS             complement(1712..3097)
                     /locus_tag="VGB68_20125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051617.1"
                     /translation="MFKYLFILGTTAILLFAQLLPASAQYICQPAANPIVIQGSINNG
                     DVQQAGRISRDGSPSTCTGDDASMENNTTLRRDTHNFVNPFNETVCVTVDLDFSACGA
                     QTQAAAYSSYNPAFPAANVIGDAGYSTINKGRFSFSVGPNAAYNIVINDIEDDGVMCA
                     LYSFKVTYQRNCRQVGFDRTNDGKADLTVYRPMSQSFWYSLNSEDNSFIARQFGTVGD
                     VVTGGSDYTGDGQSDLSLYRPNINSWIYGTDQSAPGTNFAVQQWGTTGDIRVPGDYDA
                     DSKNDIAVFRPSTGYWHILRSSDGSIQSQQWGQQNDIPLSGDFDGDMKTDFTVVRQTS
                     GGSHWYVLKSNYNYGFDEFIQFGLPTDKLIPGDYDGDAKTDFAVWRPSEGMWYVRRST
                     DLTLQATKWGQQNDIPQPADYDGDKLQDIAVWRPSNGTWYVVQSQTGTFRFQQFGQQG
                     DQPIASNMRIQ"
BASE COUNT          829 a          835 c          872 g          878 t
ORIGIN      
        1 aaatcgcagg aaaatatcgc cgtcgcgcag gcattggctg acgcgctcgg cgctgatttg
       61 gctgcttcac gcccgatttg cgacgccgaa tggctgccga ttgaccgtca aatcggctcc
      121 agcgggcaaa cggtcgcgcc gaaagtttac atcgctctcg gcatttccgg cgcgattcag
      181 cacatcgtcg gaatgaaaaa cgcgggcacc atcgtggcga ttaacaagga cggcgaagcg
      241 ccgattttcg acatcgccga ttacggaatc gtcggcgatt tgttcgaagc cgtgccggtt
      301 ttaatcgagg aaatcaaaaa ggcgaaagct tagtttaacg gaaaactgta aacgggaagc
      361 ggtaaatgta aaatcattta ccgcttcccg ttttttattt atcttacttg caagttgata
      421 taaaactgca aacagaaaag acttccgcga tttgttaccg caaatcggta acgacttcgg
      481 ggcagaccgc gcaagcctcc gcgccgctct gagaaaacca tcgacactcg gggcgtatcg
      541 agcattccgg caaattcgaa acgtcacgat tatcgcccgt cgactcgatg accgaatcaa
      601 tcacgccgca gcgatttcca gaccactgtt tgcatccgtt ttgaacgcat tgcccgccga
      661 atcgaaagcg tttttcaggc gcccgaccca gagcggcaat ttgcacaaac tcttcgttaa
      721 cgataatccg gtcgcttgaa aatgccacgc gaccgtcccg cattatgatg ccgagaagga
      781 ttgagccttc ctggcaggta tagctgggac aaagtttttt ttcagagttc attgcgttaa
      841 ctcctaaccc tgttgtattt cgttgacctc aagattgaag ttagtgtcga aggctttgtt
      901 gcggaggtaa attccgcgcc accttaactt attgggaata cgcgcggaaa aatctccgcc
      961 ggtgtcgatt ttagccactt cgcgcaggac aaccgcttga atttcgcgat tgattgatgc
     1021 ttcggcttcc ttcgataatt tcagtccgtc gatacgaacg ataaaatcag ccataatatt
     1081 ctccttagat tgtttctggt gatggaacga tgcgctttgt gaaatcaaaa caatcgccgt
     1141 cgcatcatat aacggcagtt gctcaggttg tcaggttcaa taaacagaca gaccggagca
     1201 atagtttttg cccaaatatt aaaaaaaagt ttaaatccgc gagggcatcg acaacaaagt
     1261 taaaatttcc ggcgatttgc gaaagctttc ctctactttt tttgtgttcg ggttgcggct
     1321 cgtcccgctt tgaaaggcga gatgttattc tgctattctt cgcatctcgg aactgctttg
     1381 aagaaaagtt acaatgaaac atctgaaaaa aatctttgtc gccgcttttc tcggttttct
     1441 ggcttttgcg ccgccgggaa ctttgatttt tatcggcgtt ttattgttaa gctttctggg
     1501 aaaaacgtgg tttatcgccg gtgtcgcgac gggcgcggtt ttgctggcga tttacttgtt
     1561 tatttacagg gagaaattat tgcaggcgga ttttataaaa agaatttcgc gtagatttaa
     1621 aagataaatt tttcgtaaaa aaaacttaaa gccgcgagca gtaaagaaaa cactcgcggc
     1681 tttaagtttt ctggaggcgc tgccttcacc tctattgaat acgcatattc gacgcgatcg
     1741 gctggtcgcc ctgctgaccg aactgctgga agcgaaatgt tccggtctgg ctctgaacca
     1801 cgtaccaggt tccgttcgac ggacgccaca cggcaatatc ctgaagcttg tcgccatcat
     1861 aatcggcggg ctgcggaatg tcgttttgct gtccccattt ggtcgcctgc aaggtcaaat
     1921 ccgtgctgcg gcggacatac cacatacctt ccgaaggacg ccacacggcg aaatcggttt
     1981 tcgcgtcgcc gtcgtaatcg ccgggaataa gcttgtcggt cggcaagcca aactggataa
     2041 actcgtcgaa cccgtaattg taattgcttt tcaaaacata ccagtgcgag ccgcccgaag
     2101 tctggcgcac aaccgtgaaa tcggttttca tatcgccgtc aaaatcgccg gaaagcggaa
     2161 tatcgttttg ctgtccccac tgctgcgact ggatgctgcc gtcgctgctg cgcagaatgt
     2221 gccagtaacc ggtcgaagga cgaaaaaccg cgatatcatt tttcgagtcg gcgtcgtaat
     2281 cgcccggcac gcggatgtcg ccggtcgttc cccattgctg aaccgcgaaa ttggttcccg
     2341 gcgcgctctg gtcggtgccg taaatccacg aattgatgtt cgggcgataa aggctgaggt
     2401 ccgattgtcc gtcgccggtg taatcgctgc cgccggtgac gacgtcgccg accgtgccga
     2461 actgacgggc gataaagctg ttgtcttccg aattgagcga ataccagaag gactgcgaca
     2521 tcgggcgata aaccgtcaaa tcggctttgc cgtcgttcgt gcggtcgaag cccacctgcc
     2581 ggcagttgcg ctggtaggtg actttaaaac tgtaaagcgc gcacatcacg ccgtcgtcct
     2641 cgatgtcgtt gatgacgatg ttgtaagccg cgttcggtcc gacgctgaac gaaaatctgc
     2701 ctttgttaat cgtgctgtag ccggcgtcgc cgatgacgtt ggcggcggga aacgccggat
     2761 tgtagctgga ataagcggca gcctgcgtct gcgcgccgca ggctgaaaaa tcgagatcga
     2821 ccgtgacgca gaccgtttcg ttaaacggat tgacgaaatt atgcgtgtcg cgtcttaagg
     2881 tcgtgttgtt ttccatcgac gcgtcgtcgc ccgtacaggt cgaaggactt ccgtcgcgcg
     2941 agatgcgtcc cgcctgctga acgtctccgt tgttaatcga gccctggata acaatcgggt
     3001 tcgccgccgg ttgacagata tactgcgcgc tggcgggcag cagctgtgca aataataaaa
     3061 tcgccgtggt gccgagaata aataaatact taaacattag ttttaactcc ttgtagcaaa
     3121 taaaatcgtt caatccggtc gaccttattt cgaccttcgc tattgaatgc ggaagttttg
     3181 cgcgaaaact atcatcggcg gaaaattttt tttgaaagat gaaaaaaaag gtgaattagt
     3241 ggtaagcggt aagcggtaag ttggttgctt ttgagttttg agttttaaat tttgaatctt
     3301 gaatcttgaa tgctactaga atcttgaatg ctaaatgaaa agcggtaagc ggaagcgacg
     3361 aacttaccac ttaccgctta ccgcttaccg ttgacagctg aatttattat tccg
//
LOCUS       DASWNE010001057         2210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_418629, whole genome shotgun sequence.
ACCESSION   DASWNE010001057 DASWNE010000000
VERSION     DASWNE010001057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418629"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VGB68_20130"
     CDS             <1..291
                     /locus_tag="VGB68_20130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051618.1"
                     /translation="EVENGDGLLKPGQFATVRIAQGAPKQSVMIPTVAVKTEGDTNRV
                     FVVKDGRAEERIVKLGVLENDLIEVQQGVQEGEAVATSNLNVIYDGVTVNRQ"
     gene            410..>2210
                     /locus_tag="VGB68_20135"
     CDS             410..>2210
                     /locus_tag="VGB68_20135"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY0051619.1"
                     /translation="MQWLAEVCVKRPVFATMLILSLVTVGTFSFFSLGVDLFPKIDFP
                     TITITVINPGASPEEIETEVTEKIEEAVNTISGIDELRSSSLEGISQVFVTFVLEKDV
                     NVAAQEVENRVQTVIPNLPETSEQPTVQKLDTDAAPILRIAVSAPTNLRDVTEVAKNK
                     IKERIESVNGVGQVTIIGGRERQINVWVDPDKMRSYSVTPVEITNALRIQNVEFPSGR
                     LDEGSTETSVRTVGKIKNPEEFANIVVATRGNYQVKIKDLGHVEDGAEELRTEARLNG
                     LPAVTLVISKQSGQNTVAVAQELKERLKEIEPTLPANYQMRIIGDNSIFIENSLHAIE
                     EHLVVGGLLAAVVVFLFLWNIRSTLIAALAIPTSIVSTFALMYAMGYTLNSITMLSLT
                     LMVGIVIDDAIVVLENIYRFVEEKGMDPFQAAIEGTREIGMAVLATTLSLMAVFVPIG
                     FMQGIVGRFMSSFGLTAAFAVGVSLIVSFTLTPMLAARLIKRHDSKEDPTNIYDDIAE
                     DKNEAYAHSRMAQNAAVQEIKGDGMLEGSQRDKPIAADKPKRERKDSKETGFYRYIDG
                     TYTWLLKFAMGHRWVIVALCLFVFVSIIPLFMFV"
BASE COUNT          546 a          553 c          625 g          486 t
ORIGIN      
        1 gaagtcgaaa acggcgacgg attattaaag cccggacagt ttgcgaccgt gagaatcgcg
       61 cagggcgcgc cgaaacaaag cgtgatgatt ccgaccgtcg ccgttaaaac cgaaggcgac
      121 acgaatcgcg tattcgtcgt gaaagacggc agagccgaag agcgcatcgt caagctcgga
      181 gtgctggaaa acgatttaat cgaagtccag cagggcgttc aggaaggcga agcggtagcg
      241 accagcaatc tcaatgtgat ttacgacggc gtcaccgtca acaggcaata attttttcgg
      301 tcttcggctt tcggctttcg gacttcgatt tttacttgct caaccttgag catacattct
      361 taacgaagac caaagaccaa agaccaaaga ccaaaaccga aggttttata tgcaatggtt
      421 agcagaagtt tgtgttaagc gtccggtttt cgccacgatg ttgattttgt cgctggtgac
      481 ggtcggtaca ttttcgtttt tctctctcgg cgtcgacctt tttccgaaaa tcgattttcc
      541 gacgattacc atcacggtta tcaatccggg cgcatcgccg gaagaaatcg agacggaagt
      601 gacggaaaaa atcgaagaag ccgttaatac aatcagcggc atcgacgagc tgcgctcgag
      661 ctcgctggaa ggcatctcgc aggttttcgt tacgttcgtc ctcgaaaaag acgtcaacgt
      721 ggcggcgcag gaagtcgaga atcgcgtgca gacggtcatt ccgaatctgc cggaaacctc
      781 cgagcagccg accgtccaga aactggacac ggacgccgcg ccgattctgc gcatcgccgt
      841 ctccgcgccg accaatctgc gcgatgtgac ggaagtagcg aaaaacaaaa tcaaggaacg
      901 catcgaatcg gtcaacggcg tcggacaggt aacaattatc ggcgggcgcg agcgccagat
      961 taatgtctgg gtcgacccgg acaaaatgcg ctcttatagc gtcacgccgg tggaaatcac
     1021 gaacgctctg cggattcaaa acgtcgaatt tccgagcgga aggctcgacg aaggaagcac
     1081 ggaaaccagc gttcgcacgg tcggcaaaat caaaaacccg gaagagtttg ccaatatcgt
     1141 cgtcgctacg cgcggcaatt accaggttaa aatcaaggat ttgggtcacg tcgaagacgg
     1201 cgcggaagag ctgcgcaccg aggcgcgcct gaacggtctg ccggcggtga cgctcgtcat
     1261 ttcaaaacaa tcgggacaga atacggtcgc cgtagcccag gaacttaagg aaagattaaa
     1321 agaaatcgag ccgacgctgc ccgcaaatta tcaaatgcgg attatcggcg acaactcgat
     1381 ttttattgaa aactcgctgc acgccatcga ggagcatctg gtggtcggcg ggctgctggc
     1441 ggcggtcgtc gtatttttat ttttgtggaa tatccgctcg acgctgatcg cggcgctggc
     1501 gattccgact tcgattgttt cgactttcgc gctgatgtat gcgatgggct acacgctgaa
     1561 ctcgattacg atgctcagcc tgaccctgat ggtcggcatc gtgatcgacg acgctatcgt
     1621 cgttttggaa aacatctacc gctttgtcga agaaaaagga atggatcctt ttcaggcggc
     1681 aatcgaagga acgcgcgaaa tcggcatggc ggttctggcg acgacgcttt cgctgatggc
     1741 ggttttcgtg cccatcggct ttatgcaggg catcgtcggg cgttttatgt cctcgttcgg
     1801 tttgacggcg gcgtttgcgg tcggcgtttc gttaatagtt tcgtttacct taacgccgat
     1861 gctcgcggcg cgccttatta agcgtcacga ttcgaaggaa gacccgacca atatttatga
     1921 cgatattgcc gaggacaaaa acgaggcata cgcgcacagc agaatggcgc aaaacgctgc
     1981 cgtgcaggaa atcaagggcg acggaatgct ggaagggtcg cagcgcgata agccgatcgc
     2041 cgccgataaa ccgaagcgcg agcgtaaaga cagtaaagaa acgggatttt accgctacat
     2101 cgacggcact tacacgtggc ttttgaaatt tgcgatggga catcgctggg tgattgtcgc
     2161 gctctgcctg tttgttttcg tgtcgatcat tccgctgttt atgttcgtcg
//
LOCUS       DASWNE010001058         6122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_140810, whole genome shotgun sequence.
ACCESSION   DASWNE010001058 DASWNE010000000
VERSION     DASWNE010001058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6122
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140810"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1343
                     /locus_tag="VGB68_20140"
     CDS             <1..1343
                     /locus_tag="VGB68_20140"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0051620.1"
                     /translation="ISAQDLGDLGSSSRIFRSPNPKTAKKTKPAPKTTTPKKKTPAPK
                     TETVKATPPKTNRTIATAPKPKSKPAAKNTAVKTGANKIQTIKQPLKTVKTVPDNVII
                     NVGKKTPATVDENLEEAIELGNLARDDRDYVAAERAYRRAQSIRPRDSRAIYGLGNIF
                     SDQQRWEEAEKSYRQAIALEPESPEAHIALSFVLSQPVAGINLSERYAEAEKMARRAI
                     ELDAGNAVAYDQLGVALELGGKIGIETEQAYRNAIRLDPEFALAYAHLGRLLRRNGVN
                     KESAAAYKDAIRLVSDVPTMILIAEVMQSQQRYAESEQLLRRAVREDPKNPTALYLLG
                     RALTTSGNFEEAESLLKRSIGVSPNSFIAYAQLASLYARRGNYAEAEKTLMKALKVVS
                     GNEKKQLAQEFEMVGDGFLRKGQKADAARVYRQAMTLDNEKKDLADKLTEAQKS"
     gene            1465..3795
                     /locus_tag="VGB68_20145"
     CDS             1465..3795
                     /locus_tag="VGB68_20145"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY0051621.1"
                     /translation="MFYCPKCRRTYEDGSQRFCSNDGGRLLPALPATSKSGEGKKGVF
                     STLLGKTPQKRPETDESLAPAPKFIEAEQTIQTDFPPPPASEIFPYEPKAKFEFEPKP
                     VEETKPAAETRPVAEIKPATEIKPAARLIKPSEIASGTASVGDRSVNPTGRPAISWEK
                     PNVLLGQTVKGRYQVTEKIGQDEASISYLAQDKLSPEKKVIVRVLMEEDTEDLASRIF
                     AEERVSLSHLNHPNVAGLIDSGELLEGKPFIITEHVEGKSVKDKLQQTGQFNALRAAR
                     IVRQAAYALSEVHQNGVLHRNLKPENIILTVNEAGTELVKVTDFAVSDGRQLADNPAY
                     KSPEQLEGKMPSFASDIYSLAVIAYQMLTNRLPFAASSVNDLIAAQKKGLSLKPTNLR
                     LDLPNAEEAVDRILEKALAYKPSGRYPKARDFGDAFFNALANAPNAPKTEEAENASEI
                     LPLISADTPLEDIEIEFIDDEPEETGFVAPTEPAVEEKIETEDNIKTPEDLPWEKRSP
                     EPMKVASKSWMRVSVLGLIGLVLGLWAIWYYFLNQTNRPDFSAANQAATQENSNPPVE
                     NAGATNAGPALPPDIELPPAERKVLPPQSTEYFQNTRENLQGELATYYRGFSFYYPKG
                     WVKTPGDKNFVDVARTNENGIPIEQMLVSFYKSKGTFKADREGFPALIKETNKRLNEQ
                     IPNYRFISEGEKIINGGWKVYEMKFEGTIDSNKGESIKIFGRRLFMPAMRQGIKSGFV
                     ITMLATPLSPNVQSAEDVGEKGELRTILETFEPSPM"
     gene            4057..>6122
                     /locus_tag="VGB68_20150"
     CDS             4057..>6122
                     /locus_tag="VGB68_20150"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0051622.1"
                     /translation="MKIVLAEERGGQQLSEQIFQEQTIRVGRDKSDCQIVFDNQQFPM
                     VSRKHAELRWQNGQWLLTDLGSSYGTFLNGQKISQPQAVQAGSALQFGTDGPVLRVVW
                     FEAVQPQQTPVFAPQAEPQKPISAPLPPQIPNQQQQTPNKQAPNQPAANQAGASGGSG
                     AIETPQLIFVGTSRLEPYKITKESFWLGRDPNCDIVMEANAVMVSRKHAQISRQNGGF
                     VVTDNGSFNGTLVNEQRISTPTPVYHGDEIQLGMGGPVLRFNSPSQTAPKGSSLAGQR
                     AVAAGQQAVLQDLPEQAGSKTMVFRGGNVSQQMKPDASAQPQLLMQLTFGGKQELTIG
                     RDDNNDVKLDGLQISNRHARLLQSGSSVAIEDLNSTNGVYVNGQKISRQNISTSDAVQ
                     IGSFVIRVDNAGNIGVFDTRSKTRIDSVNITKEVKDRSGGGTLKLLDGVSLTIMPNEF
                     VGLLGPSGAGKSTFMDALNGMRPASGGSVLVNNLDLYQNLDALKQSIGYVPQDDIIHR
                     ELTVYRTLYYVAKLRLSTDVSRKEIDQIVNEVMDVTGLSERRDVPINQLSGGQRKRVS
                     IAVELVTKPSVIYLDEPTSGLDPATEEKIMVLFRQIAESGRTVILTTHAMENVKLFDK
                     IVLLMRGKLVFYGKPDEALAHVGAKSFKELYDKLEEPIEKRLHQATETNQAEFKRRRE
                     QITEEVA"
BASE COUNT         1624 a         1634 c         1655 g         1209 t
ORIGIN      
        1 cgatttcggc gcaggattta ggcgatttgg gcagcagcag ccgaattttt cgctcgccga
       61 atccgaaaac tgctaaaaaa accaagcccg cgccgaaaac gacgactccg aaaaagaaaa
      121 cgcccgcgcc gaaaacggaa accgtcaaag ctacgccgcc taaaacaaac cggacaatcg
      181 cgacggctcc caaacccaaa tcaaaaccgg cagcgaaaaa caccgccgtc aaaaccggcg
      241 cgaataaaat ccagacgatt aaacagccgc tcaaaacggt gaaaaccgtg cctgacaacg
      301 ttattatcaa tgtcggcaaa aaaacgccgg ctaccgtaga cgaaaacctc gaagaagcta
      361 tcgaattagg gaacctggcg cgcgacgacc gcgattatgt cgccgccgag agagcttatc
      421 gccgcgccca gagcatccgc ccgcgtgatt cgcgcgccat ctacggatta ggaaatattt
      481 tcagcgacca gcagcgttgg gaagaagccg aaaaatctta ccggcaggcg attgcgctcg
      541 aaccggaaag cccggaggcg catatcgctc tgagctttgt tttatcccag ccggtcgcgg
      601 gcattaattt atcggagcgc tacgcggaag cggagaaaat ggcgcgccgc gctattgagc
      661 ttgacgccgg aaacgccgtg gcttacgacc agctcggcgt ggcgctcgaa ctgggcggaa
      721 aaatcggcat cgaaaccgag caggcttatc gcaacgccat ccggctcgac ccggaatttg
      781 cgctggctta cgctcatctg ggcaggcttc tgcgccgcaa cggcgtaaac aaagaatcgg
      841 cggcagccta taaagacgcg attcgcctcg tctcggacgt accgacgatg attctgattg
      901 ccgaggtgat gcagtcgcag cagcgttatg ccgaatccga gcagttattg cgccgcgccg
      961 tccgcgaaga cccgaaaaat ccgaccgcgc tttatcttct ggggcgcgct ttgacgacga
     1021 gcggaaattt cgaggaagcc gaaagtcttt taaaaagaag cattggcgtc agcccgaaca
     1081 gctttatcgc ttacgcgcag ctcgcctcgc tttatgcccg gcgcggcaat tacgcggaag
     1141 ccgaaaaaac tttgatgaaa gccttaaaag tcgtttccgg caacgaaaag aaacagctcg
     1201 cgcaggaatt tgagatggtc ggcgacggct ttctgcgaaa agggcaaaaa gcggacgccg
     1261 cgcgcgtgta tcgacaggcg atgacgctcg acaacgaaaa aaaggattta gccgacaaat
     1321 taaccgaagc ccaaaaaagt taaaacagat tattgaaaac ctcgccggat taggcgtatt
     1381 accttttaag cgttttgaga cgtttaattt ccggaagcga ggttttcaat aacttttatg
     1441 ggcgttttta taggtgaagc aacaatgttc tactgtccca agtgccgccg cacctacgaa
     1501 gatggaagcc agcgattctg ctcaaacgac ggcggacggc tgctgcccgc gctgcccgcg
     1561 acctcaaaat ccggcgaagg caaaaagggc gtgttttcga cgctgctcgg caaaacgccg
     1621 cagaagcgtc ccgaaacgga cgaaagcctc gcgcccgctc cgaaattcat cgaagccgaa
     1681 caaacgattc aaacggattt cccgccgcct ccggcgagcg aaatttttcc gtacgagccg
     1741 aaagcaaaat tcgaatttga gccgaaaccg gtagaggaaa caaaaccggc ggcggaaaca
     1801 agaccggtag cggaaataaa accggcgacg gaaataaaac cggcggcgcg tttgattaag
     1861 ccgagcgaaa tcgcttccgg cacggcttcg gtcggcgacc ggagcgtcaa cccgacgggc
     1921 agaccggcga tttcgtggga aaaaccgaac gttcttttgg ggcagaccgt caaaggtcgc
     1981 tatcaggtga cggaaaaaat cgggcaggac gaagccagca tttcgtatct ggcgcaggat
     2041 aaattgtcgc cggaaaagaa agtcatcgtc cgcgttttaa tggaagaaga cacagaggat
     2101 ttggcgagcc ggattttcgc cgaagaaaga gtttcgcttt cgcatctgaa tcacccgaac
     2161 gtcgccggtt tgattgattc gggcgagctt ttggaaggca agccttttat tatcacggaa
     2221 cacgtcgagg gcaaatcggt taaagataag ctccagcaga ccgggcaatt taacgctctg
     2281 cgcgcggcgc gaatcgtccg gcaggcggct tacgctctga gcgaagttca tcaaaacggc
     2341 gttctgcatc gcaacttaaa gccggaaaat attattctga ccgtcaacga agcgggcacg
     2401 gagctggtca aggtcaccga ttttgcggtt tccgacggca ggcagcttgc cgacaatccg
     2461 gcgtataaat cgcccgagca gctcgaaggg aagatgccgt cttttgccag cgatatttac
     2521 agtctggcgg ttatcgccta tcaaatgctg accaaccggc tgcctttcgc cgcttcttcc
     2581 gtcaacgatt taatcgcggc gcagaaaaaa ggcttgagcc ttaagccgac aaatctgcgg
     2641 ctcgatttgc cgaacgccga ggaagcggtc gaccggattc tggaaaaggc gctcgcctat
     2701 aaaccgtccg ggcgctatcc gaaagcgcgc gatttcggcg acgcattttt taatgctctg
     2761 gcgaacgcgc caaatgcgcc gaaaacggaa gaagctgaaa acgcttcgga aattctgccg
     2821 ctaatttctg cggacacgcc gctggaagac atcgaaatcg aatttatcga cgacgaaccg
     2881 gaagagacgg gtttcgtcgc cccgaccgaa ccggcggtcg aagaaaaaat cgagacggaa
     2941 gacaatataa agacgcccga agatttgccc tgggaaaaac gctcgcccga accaatgaaa
     3001 gtggcgagta aatcctggat gcgggtttcc gttttaggct tgatcggatt ggttttaggg
     3061 ctctgggcga tttggtatta ttttttgaat cagacgaatc gacccgattt ttctgcggca
     3121 aatcaggcgg cgacgcagga aaactcgaat ccgccggttg aaaacgccgg cgcgacgaat
     3181 gccggtcccg cattgccgcc ggacatcgaa ctgccgcccg ccgaacgcaa agttctgccg
     3241 ccgcagagca ccgaatattt tcaaaatacg cgggaaaacc tgcagggcga gctggctact
     3301 tattatcgcg gattttcgtt ttattatccg aaaggctggg ttaaaacgcc gggcgacaaa
     3361 aattttgtcg acgtcgcgcg gacgaacgag aacggcattc cgattgaaca aatgctggtt
     3421 tcgttttaca agagcaaggg aacttttaaa gccgaccgcg aaggatttcc ggcgctcata
     3481 aaagaaacta ataaaagact caacgagcag attccgaatt accgttttat ttccgaaggc
     3541 gaaaaaatta tcaacggcgg ctggaaagtt tacgaaatga agtttgaagg cacaatcgat
     3601 tcgaacaaag gcgagagcat caagattttc ggcagacgat tgtttatgcc cgcgatgcgg
     3661 cagggcatca agagcggttt tgtcatcacg atgctggcga cgccgctttc gccgaacgtg
     3721 caaagcgcgg aagacgtggg cgaaaaaggc gaattgagaa cgattctgga aactttcgag
     3781 ccttcgccga tgtaataaat tccggttttc cggatggttt tttgaaagcg tagggaaaaa
     3841 cttccttacg ctttttattt tgaccttagt tgcagtagaa tcttgcgata gtaaatgtca
     3901 taaattcaat tacgaattac gaattacgaa ttataatttt cgggctgtaa ttttaaacct
     3961 ctgaaactta agcaaaatca aagcccggat ttgaagttca aaggtcgttt ttccatttcg
     4021 taattcgtaa tttttaattc gtaattgcca gagaatatga aaatcgtttt agccgaagaa
     4081 cgcggcgggc agcagttgtc cgagcaaatt tttcaagagc agacaatcag agtcgggcgc
     4141 gacaagagcg attgccaaat cgttttcgac aaccagcaat ttccgatggt ctcgcgcaag
     4201 cacgccgaat tgcgctggca aaacgggcaa tggctgctga ccgatttggg ctcgagttac
     4261 ggcacttttc tgaacgggca gaaaatcagc cagccgcaag ccgtccaagc cggaagcgcg
     4321 ctgcaattcg gcacggacgg tccggttttg cgcgtcgtct ggtttgaagc cgtccagccg
     4381 cagcaaacgc cggttttcgc gccgcaagcc gagccgcaga aaccgatttc cgcgccgctt
     4441 ccgccgcaaa ttccgaatca acagcaacaa actcccaata agcaggctcc caatcagcca
     4501 gccgccaatc aagccggagc gtccggcggc agcggcgcaa tcgaaacgcc gcagctgatt
     4561 tttgtcggaa ccagccggct cgaaccttac aaaatcacca aagagagctt ctggctcggg
     4621 cgcgacccga attgcgatat tgtgatggaa gccaatgccg tgatggtttc gcgcaaacac
     4681 gcgcaaatca gccgacagaa cggcggtttt gtcgtgaccg acaacggcag ctttaacggc
     4741 acgctcgtca acgagcagcg aatttccacg ccgacgcccg tttatcacgg cgacgagatt
     4801 cagctcggaa tgggcggacc ggtactcaga ttcaattcgc cgagtcaaac cgcgccaaag
     4861 ggctcgagtc ttgccgggca gcgcgccgtc gccgccgggc agcaagccgt cctgcaggat
     4921 ttgccggaac aagccgggtc aaaaacgatg gtttttcgcg gcggcaacgt ctcgcagcaa
     4981 atgaaacccg acgcttcggc gcagccgcaa ttgctgatgc agcttacctt cggcggcaaa
     5041 caggaattga ctatcgggcg cgacgacaac aacgacgtca agctcgacgg attgcagatt
     5101 tccaatcgcc acgcgcgtct tctgcaatcc ggttcgagcg tcgcaatcga agatttaaac
     5161 tcgaccaacg gcgtttacgt caacggtcaa aaaatctcgc gccagaatat ttcgacttcc
     5221 gacgccgtgc aaatcggctc gtttgtgatt cgcgtcgata atgcgggaaa tatcggcgtt
     5281 ttcgacacgc gctcgaaaac ccgcatcgat tcggtcaata tcacgaagga agtgaaagac
     5341 cgctcgggcg gcggcacgct caagctgctc gacggcgttt cgctgacgat tatgccgaac
     5401 gagttcgtcg ggcttctcgg accttcgggc gccggaaaat cgacgtttat ggacgcgctc
     5461 aacggaatgc gcccggcttc gggcggcagc gttctggtca ataatctaga cctttatcaa
     5521 aatctcgacg cgctgaaaca atcaatcggc tacgttccgc aggacgatat tattcaccgc
     5581 gagctgaccg tctatcgaac gctttattac gtcgccaagc tgcgtctttc gaccgacgtc
     5641 tcgcgcaagg aaatcgacca gatcgtcaac gaagtaatgg acgtgaccgg cttgagcgag
     5701 cggcgcgacg tgccgattaa ccagctttcc ggcggtcagc gcaagcgcgt ctccatcgcc
     5761 gtcgagctgg tcacgaagcc ttcggtcata tatctggacg agccgacctc cgggctcgac
     5821 ccggcgaccg aagaaaaaat tatggtcttg tttcgccaga ttgccgaatc gggcagaacc
     5881 gtgattttga ccacgcacgc gatggaaaac gtcaagcttt tcgacaaaat cgttttgctg
     5941 atgcgcggga aactggtttt ttacggcaag cccgacgaag cgctcgcgca cgtcggcgcg
     6001 aaaagcttta aagagcttta cgacaagctc gaagaaccga ttgaaaaacg cctgcatcag
     6061 gcgacggaaa cgaatcaagc ggaattcaag cgccgccgcg aacaaatcac cgaagaagtc
     6121 gc
//
LOCUS       DASWNE010001059         2021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_347189, whole genome shotgun sequence.
ACCESSION   DASWNE010001059 DASWNE010000000
VERSION     DASWNE010001059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347189"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(247..1536)
                     /locus_tag="VGB68_20155"
     CDS             complement(247..1536)
                     /locus_tag="VGB68_20155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051623.1"
                     /translation="MTKKSFSVFAFLLFLTINLPAQAIQPSPMPTRARLVGSSAAPTP
                     TPSKVVTVTTTPTPRPVAAPVSNPVPVDRPVSLPTPTPPVVPQNNYKILSFGQIKTKI
                     AEAKRLMQTRPMPTGLTDGAVAMDIVRLAFYDWKTQKIDFVVLTKTAFLSKNAEVSLI
                     SSNGKVMIARIIRANGVNTPVTIYDQTGEIHTPLVVQYPVERGGRYMETAYYTSTHTG
                     VVTPEVVSAGQLYVRNTIDVARQKLSEKGIIISPQIADIAERLSTVEHVDHLRFRTEP
                     HPYIYNDIFTLYALNEGQTYRYSVSSAGAGGMVQMIPSTYYMVRNRYYSVGLMPDFVE
                     GMRNHQNAAQAMLLYMKMTWDDLIASETVYNALADGKATPSDLMAAGYNSNPAKLAGY
                     IKRGGSGWRNLIPRETKIYLQIYASLERHVPMTPRAK"
     gene            1765..>2021
                     /locus_tag="VGB68_20160"
     CDS             1765..>2021
                     /locus_tag="VGB68_20160"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051624.1"
                     /translation="MEKLFGTDGVRGLAGEFPLDEKTIEIVGRSLARQFEEKLGREPR
                     FVTGRDTRESGEWIERAFHTGAMAEGAHCESAEIITTPGVA"
BASE COUNT          483 a          471 c          577 g          490 t
ORIGIN      
        1 ggtcggaatc tgctcggaaa aactttcgcg gacgtgcgcg acgaaattgc agggaaagaa
       61 tcttagccgc gagccgggaa cgaagaaatt tagccatcgc aaaacacgga aaaagaaatt
      121 aaaaggaatt agataagata cgcggcttga taaaaacgga taaaaaagat gttttgttta
      181 tccgtatcca tcaagccgcg aagcctgttt gtccgtgtta atttttcttt ggcggctaaa
      241 gcttttctat tttgcgcgcg gcgtcatcgg aacgtggcgt tcgagcgagg cgtaaatctg
      301 cagataaatt ttcgtttcgc gcggaatcag gttgcgccag cccgaaccgc cgcgtttgat
      361 gtaaccggct aatttcgcgg gatttgaatt gtagcccgcc gccattaaat ccgaaggcgt
      421 cgcctttccg tccgccagcg cgttgtagac ggtttcgctg gcaatcaggt cgtcccaggt
      481 cattttcata taaagcagca tcgcctgcgc cgcgttctgg tgattgcgca ttccttcgac
      541 aaaatccggc atcaagccga cgctgtagta gcgattgcga accatataat aagtcgaagg
      601 aatcatctga accattccgc ccgcgccagc cgagctgacc gaatagcgat acgtctgccc
      661 ttcgttaagc gcgtaaagcg tgaaaatgtc gttgtagata tacggatgag gctcggtgcg
      721 aaaacgcagg tggtcgacgt gttcgaccgt cgaaagacgc tcggcgatat cggcaatctg
      781 cggcgagatg ataatgcctt tttcgctcag tttctggcgc gcgacgtcaa tcgtgttgcg
      841 gacgtaaagc tgcccggcgc tgacgacttc gggcgtgacg acgcccgtat gcgtcgaagt
      901 gtaataagcc gtttccatat aacgcccgcc gcgctcgacc ggatattgga cgaccagcgg
      961 cgtgtgaatc tcgcccgtct ggtcgtaaat ggtgacgggc gtgttaacgc cgttcgcgcg
     1021 aatgatgcgg gcaatcatta cttttccgtt tgagctgatg agcgagactt ccgcgttttt
     1081 tgaaagaaaa gcggtcttgg tcaggacgac aaaatcgatt ttttgggttt tccaatcgta
     1141 gaaagccagc cgcacaatgt ccatcgcgac cgcgccgtcc gtcaaaccgg tcggcatcgg
     1201 gcgcgtctgc atcaatcgtt tggcttcggc gattttggtt ttgatttgac cgaagcttaa
     1261 aatcttataa ttgttttgcg gcacgacggg cggcgtcggc gtcggcaaag atacggggcg
     1321 gtcaaccgga accggattgc tgaccggcgc ggcgaccggt ctgggagtcg gcgtagttgt
     1381 taccgtcacg acttttgaag gcgtcggcgt cggcgctgcg ctgctgccga cgaggcgcgc
     1441 gcgcgtcggc atcggagacg gttgaatcgc ctgcgccggt agattaattg tcagaaataa
     1501 tagaaacgca aaaacggaaa aacttttttt tgtcatcggg attgttcctg ttcggaaagc
     1561 ttttgcttcg cggcaagctc ggggatgaac ctgtttttaa gcgaagcacg tttccgaaaa
     1621 cgtgcgataa taaagattcg ctcaaaaacg gaatggttgt caaatataat tcaaaaattt
     1681 tatgcgaatg acaattttta tttatagcgc ggataaggta ttttggaaat ttaaatttat
     1741 ttgagttata aaagcattta ttttatggag aaattattcg gaacggacgg agttcgaggg
     1801 cttgccggcg agtttccgct cgatgaaaaa acaatcgaaa tcgtcggtcg gtcgctggcg
     1861 cgacaattcg aggaaaaact cggcagagag ccgcgttttg tgacgggacg cgacacgcgc
     1921 gaatcgggcg aatggattga gcgagccttt catacgggcg cgatggcgga aggcgcgcat
     1981 tgcgaatcgg ctgaaatcat cacgacgccg ggcgtcgctt t
//
LOCUS       DASWNE010001060         1954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_347214, whole genome shotgun sequence.
ACCESSION   DASWNE010001060 DASWNE010000000
VERSION     DASWNE010001060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347214"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..1256)
                     /locus_tag="VGB68_20165"
     CDS             complement(75..1256)
                     /locus_tag="VGB68_20165"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEY0051625.1"
                     /translation="MAKIFVNKKGARRVRGGHLWIYRSDVVKAEADGGAVVSVYDEGR
                     NFVGKAFYSDSSEITLRIFTTKDEEINKEFWRGQIKAAARRRDFFNAKTENQRPETDA
                     RRLIYSEGDLIPSLIVDDYAGVFVIQTLSQGTEKLKETFVEILREEFQPQAIIERNDV
                     KVRQRENLEMKAGVLFGDAPDEIVIEQDGVKFHVSPLGGQKTGSFLDQRENHSAMRRH
                     AFGRGLDCFTFNGGFALNLASGCESVLAIDISEDAANLARRNAELNRISNIEFQTANV
                     FDALRDFEASGEKFDTVVLDPPAFVKSRAALKSATRGYKEINLRALKLLNKNGILATC
                     SCSFHMSEELFLETVEEAARDARRRVHLIEKRTQANDHPILVGMPETYYLKCFILRVI
                     E"
     gene            complement(1266..>1954)
                     /locus_tag="VGB68_20170"
     CDS             complement(1266..>1954)
                     /locus_tag="VGB68_20170"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEY0051626.1"
                     /translation="IANQRPETKNRTLKIIFTCLLLWLAPFLVIFFAFGSDSFPFKTY
                     LFFTQAAFVTFGGAYAVLAYVNQAVVSAGWITAAQAVDGLALAETTPGPLIMVLQFIG
                     FMTGWNNPNGLNQTFHAALCALLATYVTFLPSFFFIFIGAPYIERLRNNENLTSALSG
                     VTAAVVGVILNLALMFGATVLFPDWQINYFGAALAALAFVALYFLKADVLLVVAAGGL
                     CGLAKYLLIG"
BASE COUNT          480 a          541 c          486 g          447 t
ORIGIN      
        1 cgcgaattaa aaaaaacacg aaataattta tttttctttt cgtgtttttt tgtgtctttt
       61 cgtcgccaat tctcttactc aatcacacgc aaaataaaac atttcagata ataggtttcc
      121 ggcattccga ccagaatcgg gtggtcgttt gcctgcgtgc gtttttcaat caaatggacg
      181 cggcgtctgg cgtcgcgcgc ggcttcttcg acggtttcga gaaataactc ttccgacata
      241 tgaaacgagc agctgcaagt cgccagaatc ccgtttttat tcagcagttt cagcgcgcga
      301 agattgattt ccttgtagcc gcgcgtcgcc gatttcagag ccgcccggct tttgacaaat
      361 gcgggcgggt cgagcacgac ggtgtcgaat ttttcgccgc tcgcttcaaa atcgcggagc
      421 gcgtcgaaga cattcgcggt ttgaaactcg atgttggaaa tccggttcag ctcggcgttt
      481 cgccgcgcca aattcgccgc gtcttcggaa atatcaatcg ccagaacgct ctcgcagccg
      541 ctcgccagat tcaaagcaaa accgccgtta aacgtaaaac aatccagccc gcgcccgaaa
      601 gcgtggcggc gcatcgccga atggttttcg cgctggtcga gaaacgagcc ggttttctgc
      661 ccgccgagcg gcgagacgtg aaattttacg ccgtcctgtt cgatgacgat ttcatcgggc
      721 gcgtcgccga acaaaacgcc cgctttcatt tccagatttt cgcgctggcg gactttgacg
      781 tcgtttcgct cgataatcgc ctgcggctga aattcttcgc gcaggatttc gacgaaagtt
      841 tccttgagct tttccgtgcc ctgcgaaaga gtctgaatga cgaaaacgcc cgcgtaatcg
      901 tcgacaatca gcgacggaat caaatcgcct tccgaataaa tcagacgccg cgcgtcggtc
      961 tccggtcttt ggttttcggt cttcgcgtta aaaaaatccc gccggcgggc ggcggctttg
     1021 atttgcccgc gccagaattc cttgtttatt tcttcatctt tcgtggtgaa aattcgcagc
     1081 gtgatttccg acgaatcgct gtaaaacgct ttgccgacaa agtttctccc ttcgtcgtaa
     1141 acggagacga ccgcgccgcc gtcggcttcc gctttgacga catcgcttcg ataaatccat
     1201 aaatgaccgc cgcgaacacg gcgcgcgcct tttttattga cgaaaatttt tgccatcaat
     1261 caaatttaac caatcaaaag atatttcgcc aatccgcaca aaccgcccgc cgcgacgacc
     1321 agcaagacgt ccgccttcag aaaatacagc gcgacaaatg ccagagccgc cagagccgcg
     1381 ccgaaataat tgatttgcca atcgggaaaa agcaccgtcg cgccgaacat cagcgccaga
     1441 ttcagaatca cgccgacgac cgccgccgtc acgcccgaca gcgccgaagt caggttttcg
     1501 ttatttcgca ggcgttcgat ataaggcgcg ccgataaaaa taaaaaagaa cgacggcaga
     1561 aaagtgacgt aagtggcgag cagggcgcag agcgccgcgt gaaaagtctg gtttaatccg
     1621 ttcggattat tccagccggt cataaatccg ataaactgca aaaccattat caaaggtccg
     1681 ggcgtggttt ccgccagcgc caagccgtcg accgcctgcg ccgccgtaat ccagcccgcg
     1741 ctgacgaccg cctgattgac gtaagccaga acggcatacg cgccgccgaa agtgacaaac
     1801 gccgcctgcg tgaaaaaaag atatgtttta aacggaaaac tgtcggagcc gaaagcgaaa
     1861 aaaattacga gaaacggcgc gagccagaga agcagacagg tgaaaatgat ttttaacgtc
     1921 cggtttttgg tctccggtct ttgatttgcg attt
//
LOCUS       DASWNE010001061         3835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_176132, whole genome shotgun sequence.
ACCESSION   DASWNE010001061 DASWNE010000000
VERSION     DASWNE010001061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176132"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..690)
                     /locus_tag="VGB68_20175"
     CDS             complement(10..690)
                     /locus_tag="VGB68_20175"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY0051627.1"
                     /translation="MKANTDKKPVCLDAPKRGETSGFSLIELIIVLVIMMILSAISLP
                     YIYNYQKRYKSEDQALIVMDFMREANQLALNKRRTMRLEIDLTDNKLLLIDEKEAGAG
                     DDTEVKAIPLELTNEVRMDQIPTGVSKPNPPNYNDAVFAADTIGHKSAGTTVTGHRVW
                     AIKFKSDGSVVNAAGTLTSANLYVWAPVAAGSATPRSLKEVRVITIFGGSGAVRYWKH
                     NGTTFVAY"
     gene            1042..2244
                     /locus_tag="VGB68_20180"
     CDS             1042..2244
                     /locus_tag="VGB68_20180"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY0051628.1"
                     /translation="MAENKTSPPKKSFSADWLVGGILAKLGESFDRLTGRNWKPSSSL
                     ATSELIERLKKLLDSKVRDLGEHGRFVPHNIKLKMQWDKFSTDAEKALEKLQTELHAA
                     AIDHINDNRYHTYAPIKVEVKPDYFTEGVNLHAGFEKFGEQEESEEDILKVTMPQISL
                     QNIVINPEPEPEPEPEKFVAAFKVAGREKSIELEFKLSQRRNVGRTKENDLMLDDASV
                     SKIHASLVLNGENQLMVADTGSTNGTFINNQRIAYGKASMINDGDRVKFGTVEVFFRR
                     VPKPVESESRENFETQAFKAENPQVIIKPQAVEAPRTVIQEAVKQSPAPEQNPAATEI
                     YKTGEEAEIFKSDGNFEITNGANESANESQNNQTDGREFQNNQNDSRVFQTEQRIKLD
                     FGDEGNKN"
     gene            2247..>3835
                     /locus_tag="VGB68_20185"
     CDS             2247..>3835
                     /locus_tag="VGB68_20185"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY0051629.1"
                     /translation="MFYLFLFLQFDWQKLRPENLFGGQVLERTPAGLSIIYLVGITIL
                     IAFLMLSFVRNLRPKFAFERDLPKEVKRKLSMTQTNRSLRIWQMVFIVLAFTVFGFHV
                     YWTLYAGKDNERFQELTNRDLRSRRTASSQLRGWMLDRSGKLGNALAYYKREQDGDIV
                     RAYSLEKEMAHLLGTERSTPGLERTLYKRVADPMPEAWEVLTRIKKPEDEQRDVRVTI
                     DRDLQAFLAEQLQGKKGAVVVLNPQTGELLAMYSNPSFSLGEVENLDSFYQLEHDKRN
                     KPLLNRATREFYVPGSTFKTFTMISAFRAGMQNTTLQSTPGGYVAFRGSRAITDANGG
                     CEPPYGCGVLNIAQAYEASSNQYFAQMAVNLGRERIRETAGLLGIAAVDTPAEAVQSK
                     FFSDIWNTSNKRIASSMALQQSTIVTGKNISAYDIGLEGMGQGYAGQMTPFQMALIAA
                     ATGNMEGKLMKPKIEFDQPPQAFAQILSPQQAAAVRDIMGLVTEGSGGTATRVFAAVR
                     AAGIRTGGKTGTAEKLAPVYD"
BASE COUNT          979 a          992 c         1014 g          850 t
ORIGIN      
        1 tcgctcgaat taataagcta caaaggttgt gccgttgtgt ttccagtatc tgaccgcgcc
       61 gctgccgccg aatatcgtga tgacgcgcac ttctttcagg cttcgcggag tggcgcttcc
      121 ggcggcgacc ggcgcccaga cgtatagatt ggcgctggtc agcgttccgg cggcgttgac
      181 gaccgagccg tcgcttttga atttaatcgc ccagaccctg tgacccgtga cggtcgtgcc
      241 ggcgctcttg tgtccaatcg tgtcggcggc gaaaaccgcg tcgttgtaat tcggcggatt
      301 cggtttggag acgccggtcg gtatttgatc catcctgact tcgttggtca attcgagcgg
      361 gattgcttta acttcagtgt cgtcgccggc gccggcttct ttttcgtcga tgagcagcag
      421 cttattgtcg gtcaaatcga tttccagacg catcgtccgg cgcttgttta aagcgagctg
      481 atttgcctcg cgcataaaat ccatcactat cagcgcctgg tcttccgatt tgtatctttt
      541 ctggtagtta tatatataag gtaatgaaat ggcggaaaga atcatcataa tgacgagcac
      601 tataatcagc tcgatcaacg agaaaccgct cgtttcgcct cgtttcggcg cgtcgagaca
      661 aacaggtttt ttatcggtat tggctttcat cgtggtaatt attcaatcta tgcaagtcct
      721 atcgggtatg ataaatcggc atcgctttcg ctcgcgaaaa gctcctctct gacaaatata
      781 atctcgctgc agggcaaccc gtggagctga atttgatgat taacggtgag tttttaattc
      841 aatttgaccg agcctgaatg aaaaaacttc aatctgactc agtaataatt ataccaacaa
      901 tgcaaaacat tttccaacaa aatttttaaa acggtgaaaa actataaaaa taagcgaacg
      961 gattgcttcc atttaacggg cgtttcggat aaaattacgg cacgtttagg gaaaattttt
     1021 cttccgtaga aacttcggga aatggctgaa aataaaacat caccaccaaa aaaatccttc
     1081 tcggcagact ggctggtggg cgggattctg gcaaagctcg gcgaatcgtt cgaccggctg
     1141 acgggcagaa actggaagcc ttcgagcagt ctggcgacca gcgaattgat tgagcgtcta
     1201 aaaaaactgc tcgactcgaa agtccgcgat ttaggcgagc acggtcgttt tgtcccgcac
     1261 aatataaagc tgaaaatgca gtgggacaaa ttttcgaccg acgccgaaaa ggctctcgaa
     1321 aaactgcaaa ccgagctgca cgccgcggcg attgaccaca tcaacgacaa tcgctatcac
     1381 acctacgcgc cgataaaggt cgaggtcaag cccgattatt ttaccgaggg cgtaaatctg
     1441 cacgccggtt tcgaaaaatt cggcgagcag gaagaatccg aagaagacat tctgaaagtg
     1501 acgatgccgc agattagtct gcaaaacatc gtgataaatc ccgaacccga accggagccg
     1561 gaacccgaaa aattcgtcgc cgcgtttaag gtcgccggca gagaaaaatc aatcgagctg
     1621 gaattcaaat tgagccagcg gcgcaacgtc ggcagaacaa aagaaaacga tttgatgctc
     1681 gacgacgcga gcgtttccaa gattcacgcg agccttgttt taaacggcga aaatcaatta
     1741 atggttgccg atacgggctc gaccaacgga acttttatca acaatcagcg tattgcttac
     1801 ggcaaggctt ctatgataaa cgacggcgat agagtgaagt tcggcacggt cgaggttttc
     1861 ttccggcgcg ttccaaagcc ggtcgaatcg gagtcgcggg aaaatttcga aacgcaggct
     1921 ttcaaagcgg aaaatccgca ggttatcatc aaaccgcaag ctgtcgaagc tcctcgaacc
     1981 gtcattcagg aagcagtcaa acaaagtccc gcgccggagc aaaatccggc ggcgaccgaa
     2041 atttataaaa ccggtgaaga agcggaaata tttaaatccg acggaaattt tgaaataacc
     2101 aacggcgcga acgaaagcgc gaacgaaagt cagaataatc aaacggacgg tcgggaattt
     2161 caaaataatc aaaacgacag ccgggttttt caaaccgagc agcgcataaa gctcgatttc
     2221 ggcgacgaag gcaataaaaa ttaaagatgt tttacttatt tcttttttta caattcgact
     2281 ggcagaagct tcgaccggaa aatcttttcg gcggacaggt tctggagcgg acgcccgccg
     2341 gcttaagtat catttatctg gtcggcatca cgattctgat tgcgtttctg atgctttcct
     2401 ttgtccgcaa cctgcgcccg aaattcgctt tcgagcgtga tttgccgaag gaagtcaaac
     2461 gcaaattgag tatgacgcag accaatcgca gcctgcgcat ctggcagatg gtctttatcg
     2521 tgctggcgtt taccgttttc ggttttcacg tttactggac gctctacgcc ggtaaggaca
     2581 acgagcgttt tcaggaattg accaatcgcg atttgcgctc gcggcgcacg gcttcctcgc
     2641 aactgcgcgg ctggatgctc gaccgctccg gcaagctcgg caacgcgctc gcctattaca
     2701 agcgcgagca ggacggcgac atcgtgcgcg cttactcgct ggaaaaagaa atggcgcatc
     2761 ttctgggcac ggagcgttcg acgcccggac tggagcgcac gctttataaa cgagtcgccg
     2821 acccgatgcc cgaagcgtgg gaagttttga ccagaatcaa gaaacccgaa gacgagcagc
     2881 gcgacgtccg cgtgacgatt gaccgggatt tgcaagcctt tctcgcagag cagttacagg
     2941 ggaaaaaagg cgcggtcgtc gttttgaatc cgcagacggg cgagcttttg gcgatgtatt
     3001 ccaatccttc attttcgctc ggcgaagtcg aaaatctgga cagtttctac cagctcgaac
     3061 acgacaagcg caacaaaccg cttttaaacc gcgcgacccg cgaattttac gtgcccggct
     3121 cgacctttaa aactttcacg atgatttccg cttttcgcgc cggcatgcag aatacgactc
     3181 tgcaaagcac gccgggcggc tacgtcgcct ttcgcggctc gcgcgcgatt accgacgcca
     3241 acggcggctg cgagccgcct tacggctgcg gcgtcctgaa tatcgcgcag gcttacgaag
     3301 cttcgagcaa ccagtatttc gcgcagatgg cggtcaatct cggacgcgag agaatccgcg
     3361 agaccgccgg gctgctcggc atcgccgccg tcgatacgcc agccgaagcg gttcagtcaa
     3421 aatttttctc ggacatctgg aacaccagca acaagcgcat cgccagttct atggcgctgc
     3481 agcaatcgac catcgtgacc ggcaaaaata tttcggcata cgacatcggg ctggaaggaa
     3541 tgggacaagg ttacgccgga cagatgacgc cgtttcagat ggctctgatt gccgcggcga
     3601 ccgggaatat ggaagggaaa ctgatgaagc cgaaaatcga gtttgaccag ccgccgcagg
     3661 ctttcgcgca gattctctcg ccgcagcagg cagccgccgt ccgcgacatt atgggtctgg
     3721 tcacggaagg ctcgggcgga acggcgacgc gggttttcgc agccgttcgc gccgccggaa
     3781 tccgcacggg cggcaaaacc ggaacggcgg aaaagctcgc tccggtttac gacga
//
LOCUS       DASWNE010001062        15348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_140993, whole genome shotgun sequence.
ACCESSION   DASWNE010001062 DASWNE010000000
VERSION     DASWNE010001062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 15348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15348
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140993"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..1082)
                     /locus_tag="VGB68_20190"
     CDS             complement(51..1082)
                     /locus_tag="VGB68_20190"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY0051630.1"
                     /translation="MAAQDFYLNRPRRAMRPKLGTWLRHLLLLSLTLVTATIAGVLQP
                     FGSKPIFEEIPVETWSDVVNFFYTLPQNYFKLIVETLQELATEPELLKHGLSFSASLL
                     FILTAHESGHYIACRIYGVDATLPYFIPTPPMIGPAGTLGAFIKIMSPMPSRRAVFDI
                     GVAGPIAGFIALIPVAVIGFLTWRYAPPAAPNASLDLSLGFVFSDPLMMQFLAWISNV
                     DLSRATRANPFYFAAWLGLLVTALNLIPSGQLDGGHAVYAVLGEKVHWWTGRIAFAGM
                     ALLSIAGFYFYNSPSGFLFAVLLGVMMRIRHPEPLDNTPLDFKRKAVALLVLLIFVLC
                     FVPFPIQIR"
     gene            complement(1151..1432)
                     /locus_tag="VGB68_20195"
                     /pseudo
     CDS             complement(1151..1432)
                     /locus_tag="VGB68_20195"
                     /inference="COORDINATES: protein motif:HMM:NF037081.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
     gene            complement(1588..2454)
                     /locus_tag="VGB68_20200"
     CDS             complement(1588..2454)
                     /locus_tag="VGB68_20200"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0051631.1"
                     /translation="MNLNDWQASGKFFDYKGFPIFYRQSAASREVLLCLHGFPSSSHD
                     YHKIWDGLAKEFPLAAFDMPGYGFSAKPAHFDYTTFNQADVLQALAENLKIKKIHLLA
                     HDYGNTITQELLARRAENRLNFTIESICFLNGALFPETHKPILAQKLLISPLGALFGK
                     FISDSRFKQSLATVFGADTQPTAAELNDFLAVFKFNDGRRIAHKLIRYMRERRLYRER
                     WVSALDSINVPFRFIDGLADKVSGAHLVKRFREVCPQHTDIIELENIGHYPHFEAPAK
                     VLQKYTEFRKRQ"
     gene            2521..3351
                     /locus_tag="VGB68_20205"
     CDS             2521..3351
                     /locus_tag="VGB68_20205"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEY0051632.1"
                     /translation="MLRGRESTNVEDRRGMGGKGIALGGGGCGTLLIIILALIFGVDP
                     RQFLDTGTQPEAPSTQTQQQQPGNKAADEDGSFARSVLGSTEDAWREILPQQARIKYR
                     DPKLVLFTNQVQSACGTAGSSTGPFYCPGDEKLYLDFGFFRELKNEFKAPGDFAQAYV
                     IGHEVGHHVQNLLGTMDKVNQAGNTNALSVRLELQADCYAGVWANYAQKKGQLETGDV
                     EEALRAASAVGDDAIQKRAQGYVVPESFTHGSSKERMQWFTRGFQAGDMRQCNTFATR
                     "
     gene            complement(3614..4147)
                     /locus_tag="VGB68_20210"
     CDS             complement(3614..4147)
                     /locus_tag="VGB68_20210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051633.1"
                     /translation="MAQAQTENFPPKLEKNPSGDINVNSLANLLEWFLNYDPKVALVR
                     HAHVEELFEWKQADDAAQGIEVYPFENAEARFAIGAFQAIAENDTEAKLQRWITDVIQ
                     ALGESKQTREDTVAEYKLETNEGASFVMESQKIPSKTEQRLFLTSAWLEALCMAEARF
                     LGWVYQELYGKPFQISD"
     gene            complement(4214..5224)
                     /locus_tag="VGB68_20215"
     CDS             complement(4214..5224)
                     /locus_tag="VGB68_20215"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HEY0051634.1"
                     /translation="MSHRIGEDNNGDNGAITAATLSAIAYCDGADSIGKSLASSLPGW
                     ALAWIPDVEINGNYAFIAYNQHVQQYAVAIRGSVLDFSWQAFEDWFEQDFNVLYQSAW
                     TYPTSDQYPMISRGASDGLSDLTSLVSTVSAGTPVRMLDFLMGHAVENNIPILVTGHS
                     LGAGLSTVFAPWLYYQIKSAQKQVPDMAVMTFAAPAVGNQAFAAAFDATFPNSWRYYN
                     SQDIVPMASASIADMGNLYSPAPEASSISTTYDGYTVTLQEGIIALDAAVIASEVYNN
                     SVYAQTNGASGSVELNQAGKLFKVTATDPLEQWFEQAGAQHSLSNAYLPFLGGQPFAC
                     SS"
     gene            complement(5453..6466)
                     /locus_tag="VGB68_20220"
     CDS             complement(5453..6466)
                     /locus_tag="VGB68_20220"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEY0051635.1"
                     /translation="MKSEILAKVIRGETVESIHRGHLIVVDGAGETLFSLGNPATVTF
                     FRSSAKPFQAFPFLTSGAAERFGFLENEIALACGSHSGEKYHVETSRKMLARIGLSEA
                     DLRCGAHPPFDENRAEEILRNGEKTTQLHNNCSGKHAAMLALAKHIGADLATYEYFEH
                     PVQQRVLEVVSKFTGVAKDEIKLGVDGCAAPNFALPVAAMARSFARLVLPPADFDAET
                     REACRRIVSAMISYPEMIGGRKRLDTLLMLEANRRLISKIGADGVYAAGVLPSPQWKK
                     GLGIAFKIEDGEDKRARPVVAIELFRQLGIFSAKALADLSPLPVKNRRGDTVGRVEAS
                     FRI"
     gene            complement(6600..8300)
                     /gene="recN"
                     /locus_tag="VGB68_20225"
     CDS             complement(6600..8300)
                     /gene="recN"
                     /locus_tag="VGB68_20225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEY0051636.1"
                     /translation="MLSLLKIKNIALIDELELEFARGLNLLTGETGSGKSIIVDSLGA
                     LTGERVSSDLIKEGTQNAQIEGLFQIAASDELNKIFDESGVEFDAGGEVELIVRRELS
                     ATGKNRIFVNNQLVTQSFLKRIGAFLVDIHGQGEQATLFNPATHLEMLDEYANLESLR
                     GKVSEKYRELAAIQRELRNLREDEAQKLQLLDILCFQVEEIGKANLKTGEDDALEEEK
                     RRLNNVEKLSTLSDESYSLLYENEEATVTTLEKAIRRVTELAEYESSFAAYMEGLQTA
                     QAVLEDLAMSVRDFRNRLEFSPERLEEIENRLAEISRLKRKYGGSVESVLAHLSESEA
                     RLSNIETAELREEELRKKLKKSREEYLQIAVELNEKRSKAARKFEKDVEANLKAVALE
                     KARFEVRIDAPNNLQAAETDKSFTAKGFDQIEFYFSANVGESVKPIAKVASGGEASRL
                     MLILKTTAKLSEREKAMVFDEIDVGIGGRVAEAVGLKLKELAKTQQVLCVTHQPQVAS
                     LADHHFLVEKQAAKNRTEIRIKELSKTEKVEEIARMLTGKEITETARRHAKEMLSASR
                     "
     gene            complement(8549..9871)
                     /locus_tag="VGB68_20230"
     CDS             complement(8549..9871)
                     /locus_tag="VGB68_20230"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEY0051637.1"
                     /translation="MKPLSFFLLTLLALFLHIEMRAQSAQDFGKIWDKQHISTLPPSQ
                     VRHKDLQNYLEKLRALGLKVEEVGRSYANREIYQIEFGKGATRVFMWSQMHGDEPTAT
                     PALIDMLAFLQTNRDKKWVREIEEKLTIRAVPMLNPDGAELFQRRNLQSIDINRDARA
                     LQTPEGRLLKKLRDEWQPQIGFNLHNQNPLTAAGNTNRQAVISLLAVSGNPEGKSDEK
                     HQRSRRICAVMVEALSHFIKGGVGRYDDAYNARAFGDNFSAWGTPAILIETGALYGKD
                     EMFLVKINFVAYLAALRSIVTGSETKAEMSVYDTLPFNDVGNLYNVLFRKATVVNLSE
                     NGRTFMSDLGTAFDRRRAGEETTLTVRELGDMSIGAYGLEEYNVENFYLVPKSGAWRV
                     GIPADFLFYKKSRQIDWKAADLETRFPPDAIYKNGKWLKGEKVVPRMN"
     gene            complement(10129..10989)
                     /locus_tag="VGB68_20235"
     CDS             complement(10129..10989)
                     /locus_tag="VGB68_20235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051638.1"
                     /translation="MKYCPNCQSTYTDDTLQFCLQDGTVLVNASETPITIGWKDTEAP
                     TVVRPTVSTQQNQLPSADTPPPAAKKSRTGLVIFLTALTTLLLFGAGIGAWYLLRNRG
                     AEVAINSSVNNSPKPANKNSSNANANKGNANASPTPSPANTNANANSSPTPNIDAGQI
                     KSDVSEKVNGWAQSLEAGDLSAHMSNYADRLDYYYTARDVSLGAVRSDKQRAFGSFDD
                     FRMEISNLRVTPDADGEKATAVFDKEWEFEGAENRNKGKAQSQLQLTKIDGDWRITGE
                     RDLKVYYTEK"
     gene            11264..11887
                     /locus_tag="VGB68_20240"
     CDS             11264..11887
                     /locus_tag="VGB68_20240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051639.1"
                     /translation="MKFKISQKIIALSFVITFAFLVFSCQPSQQQQVGSAQTPTEAYK
                     MLYAAVKAKDTEKIKKMMSKDTMILAEGAAKQQNKTLESVLENGFYASTFSATLPKMR
                     DERIKENYGAVEVWNEKERLWEDVAFIKEEDGWKVAVGDIFKGTYQVPGKSQSIRERE
                     DANTMNPNNAVTQANINTGVNTNAIPVTNVKVPPPPLANKDDGAKKK"
     gene            11884..12222
                     /locus_tag="VGB68_20245"
     CDS             11884..12222
                     /locus_tag="VGB68_20245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051640.1"
                     /translation="MISVEQILEIISQYKKHGWTLRRVLLSDELRVRAYNELVALSTG
                     GAVEFVSSEVEAVWFSRPAQAAGEAWELRHLSSTPFALFEVFDADDEEEERDEARKEM
                     ETRLKLSVIK"
     gene            12306..12911
                     /locus_tag="VGB68_20250"
     CDS             12306..12911
                     /locus_tag="VGB68_20250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051641.1"
                     /translation="MIKDFGARVEKAWEGLRPMTKKMLVGAIEANAANLPKQKFSYDA
                     HADWELSRLLTALDEQASDTEIRKDAEKLREIKQLAETCINVLQTQTESAEVFIQLAE
                     RALKKNDFRKIDVLADILSHRFSVGEMCEIVRQTTNPGIRALGFEALALVPVSALMQM
                     LDDPVYFEIVRNVLEQQAFEYESEEARRVLEQLDFEEMNGE"
     gene            complement(12999..13751)
                     /locus_tag="VGB68_20255"
     CDS             complement(12999..13751)
                     /locus_tag="VGB68_20255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051642.1"
                     /translation="MSNLSDIGFPVRGEQDVNDVIMKVLKNVSEIPCPRGSYYKFADA
                     SGAAIYLQANASQELIGFNPHFEGKSRRTVCLTETIEKESSELDGAFHAWANPSEENN
                     PESGEYPFVFDVPDFRVNTDLNFPQNRQVQLTAFASNEFQVFASERDFYAQQDSELKF
                     AAKSFVPTGLINFHIDNTQPPLAFGVFTGEIKEFELKTNELTGEKFYWFLVDTLGGEI
                     DVVADAKLVAAEPSVGGYLNGQFWLSGRLLAK"
     gene            complement(13981..15279)
                     /gene="glyA"
                     /locus_tag="VGB68_20260"
     CDS             complement(13981..15279)
                     /gene="glyA"
                     /locus_tag="VGB68_20260"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEY0051643.1"
                     /translation="MNNFFTADLAAADPIISEAIDSEVRRQTDGLELIASENFVSEAV
                     LEAMGSVFTNKYAEGYPAKRYYGGCEFADVVENTAIERAKQLFGADHANVQPHSGAQA
                     NMTVLLAALEHGDQILGMNLSHGGHLTHGHPLNFSGLNYKVADYGVSRETETIDYDEL
                     QRIAEENKPKLIICGASAYPRTIDFQRIGEIAKSIGARVMADIAHIAGLVAVGLHPSP
                     VPHAEYVTTTTHKTLRGPRGGLILCREEYAGDVNKRLFPGVQGGPLVHIIAAKAVAFG
                     EALKEDFKTYQQQVLANAQALSAELQNQGLRIVSGGTDNHLMLVDVFMGGKGITGKAA
                     EKALDEVHITVNKNTIPFDPNKPFVASGIRLGTPALTTRGMKEAEMKQIAGLIASVIH
                     EAESEEVRRRVKREVAELTMRFPMYPNRYKEAKIESISAS"
BASE COUNT         3729 a         4021 c         3872 g         3726 t
ORIGIN      
        1 aagaagcaaa gcaaataaag gcgcggcgaa cgtgttttga gcggctaaaa ttatctaatc
       61 tgaatcggaa acggaacgaa acataaaacg aaaatcagca ggaccagcaa ggcgaccgct
      121 tttcgcttga aatcgagcgg cgtgttgtcg agcggttcgg gatgccggat gcgcatcatt
      181 acgccgagca gaaccgcaaa cagaaaaccg ctcgggctgt tgtaaaaata aaatccggca
      241 atcgaaagca aagccattcc ggcgaaagcg attctgcccg tccaccaatg aactttctcg
      301 cctaaaacgg cgtaaacggc atgcccgccg tcgagctgcc cggacggaat caggtttaaa
      361 gccgtcacca gaagccctag ccacgccgcg aaataaaacg gattcgcccg cgtcgcgcgg
      421 ctcaaatcca cattggaaat ccaagccaga aactgcatca ttaaagggtc ggaaaagacg
      481 aatcccagac ttaaatcaag cgaagcattc ggcgcggcgg gcggcgcata ccgccacgtt
      541 aaaaagccga tgacggcgac cggaatcagc gcgataaatc cggcaatcgg accggcgacg
      601 ccgatgtcga aaacggcgcg gcgcgaaggc atcggcgaca taattttgat aaacgctccc
      661 agagttccgg caggtccgat catcggcggc gtcgggataa aatacggcag agtcgcatcg
      721 acgccgtaaa tccggcaggc aatataatgc ccgctctcgt gcgccgttaa aataaaaaga
      781 agcgacgcgg aaaaactcaa accgtgtttt aaaagctccg gctcggtcgc caattcctga
      841 agcgtctcga cgataagctt aaaataattc tgcggcagcg tgtagaaaaa attgacgacg
      901 tccgaccacg tctccaccgg tatttcttca aaaatcggtt tcgagccgaa aggctgcaaa
      961 actccggcaa tcgtcgcggt caccagcgtc agcgaaagca aaagcagatg cctgagccag
     1021 gttccgagct tcgggcgcat cgcgcggcgc gggcggttaa ggtaaaaatc ttgagctgcc
     1081 ataaaaaagg taaatcgtaa acagtttaag ctaaccgttt acgatttacg attgggaaac
     1141 tacaatttgc ttattttttt acagccttgg cttgaagctc cttgccgggt ttgaaacgaa
     1201 tcgttttgcc cgacggaatc ggcacttctt tgcccgtccg cggattgcga ccaattccgc
     1261 gtttgcgcgg tttcacgacg aaaacgccga aaccgcgaag ttctattctt ttgccctttg
     1321 ccagcgcgtc tttcattgaa ttaaaaagcg aatcgaccac ttgttccgcc ttctgcttcg
     1381 gcacgcccgt tttttccgca acgcggttga cgatgtctaa tttaatcatt tccttctcct
     1441 taaaatgttg gaagaaaaac ctttaaccga cgtagagtta tataataaaa acctgaatgc
     1501 cttctgtcaa gatttttggc ggttaaaatt aagaaaagaa aaagtttaat tcatttttaa
     1561 agccaaaact caagataaaa taaagcgtta ttgtcttttc ctgaactcgg tatatttttg
     1621 taaaactttg gcgggcgctt caaaatgcgg gtaatgaccg atattttcaa gctcaataat
     1681 gtccgtatgt tgcgggcaaa cctcgcgaaa tcttttcacc agatgcgcgc ctgaaacttt
     1741 atccgccagc ccgtcgataa agcgaaacgg cacgtttatc gaatcgagag ccgaaaccca
     1801 gcgctcacga taaaggcggc gctcgcgcat atatcgaatg agcttgtgcg cgattcggcg
     1861 tccgtcgttg aatttgaaaa cggcgagaaa atcgtttaat tccgccgccg tcggctgcgt
     1921 atccgcgccg aaaaccgtcg ccagactttg cttgaagcgc gaatcggaaa taaatttgcc
     1981 gaacagagcg ccgagcggag aaatgagaag tttttgcgcc aaaatcggtt tatgcgtttc
     2041 gggaaacagc gcgccgttta gaaaacaaat gctttcaatc gtaaaattca ggcgattttc
     2101 cgcgcgccgc gccagcagct cctgcgtaat cgtattgccg taatcgtgcg cgagaaggtg
     2161 aatttttttg attttcaggt tttccgccag cgcctgtaaa acgtccgcct gattaaaagt
     2221 cgtgtaatcg aaatgcgcgg gcttggcgga aaaaccgtaa ccgggcatat cgaaagccgc
     2281 cagcggaaat tccttcgcca gcccatccca gattttatga taatcgtgcg aggaagacgg
     2341 aaaaccgtgc aggcacagca aaacctcccg ggaagcggcg ctctgccggt aaaaaatcgg
     2401 aaaacccttg taatcgaaaa actttccgct cgcctgccaa tcgtttaaat tcattgatta
     2461 ttccgttcaa aatgttatat ttctacaata taataaattt tcgagtaagg ggtggaattt
     2521 atgttaagag gtcgtgaaag cacgaatgtc gaagaccggc gcggaatggg cggcaaagga
     2581 atcgcgctcg gcggcggcgg ctgcggcacg ctgctgataa ttattctcgc gctgattttc
     2641 ggcgtcgacc cgcgccagtt tctggatacg ggaacgcagc cagaagcgcc gtcgacgcaa
     2701 acacagcagc agcagcccgg caataaggcg gcggacgagg acggcagttt tgcgcggtcg
     2761 gtactgggaa gcaccgaaga cgcctggcgc gagattctgc cgcagcaggc gcgaatcaaa
     2821 taccgcgacc cgaagctcgt tctttttacc aatcaggttc aatcggcttg cggcacggcg
     2881 ggctcttcga ccggaccttt ttattgtccg ggcgacgaaa agctttatct ggatttcggt
     2941 ttcttccgcg agctgaaaaa tgaattcaaa gcgcccggcg attttgccca agcctatgtt
     3001 atcggtcacg aggtcgggca tcacgtccag aatcttttgg gcacgatgga taaagtaaat
     3061 caagccggaa acacaaacgc gctttccgtc cggctcgagc tgcaagcgga ttgctacgcc
     3121 ggagtctggg caaattacgc gcagaaaaaa ggtcagctgg agaccggcga cgtcgaagaa
     3181 gcgctgcgcg ccgcgtcggc ggtcggcgac gacgcgattc agaaacgcgc gcagggctac
     3241 gtcgtcccgg aatctttcac gcacggctct tcgaaagagc gtatgcagtg gtttacgcgc
     3301 ggatttcaag cgggcgatat gcgtcaatgc aacactttcg cgaccagatg attctagctg
     3361 aaaagttatg aaaagctttt tcagtcaatc atagaaaaat tcggcggctg caagataaat
     3421 tgcagccgct tttttaatgc tcgctcaaaa cattcggaaa taagaaaacg agcttacgct
     3481 tatattaacg aaaaaggcaa aaaatctttg tctatgcgat tgaagatgaa atgtaaatta
     3541 gagccggaaa attataaaat ttgattcaga ccggagcttg gacgctttcg aatttatcat
     3601 ccggcaatga catttaatcg ctaatctgaa acggcttgcc gtagagttcc tggtaaaccc
     3661 agcctaaaaa tctcgcctca gccatacaga gcgcttcgag ccacgcgctc gtcaaaaata
     3721 aacgctgctc ggttttggaa ggaatttttt gcgattccat cacaaacgaa gcgccctcgt
     3781 tcgtttccag cttgtattcg gcgaccgtgt cttcgcgcgt ttgtttcgat tcgccgagcg
     3841 cctgaatcac gtccgtaatc cagcgctgca atttcgcttc cgtgtcgttt tccgcaatcg
     3901 cctgaaaagc gccgatagca aaacgcgcct cggcattttc aaacgggtaa acctcgattc
     3961 cctgcgcggc gtcgtccgcc tgtttccact cgaaaagctc ttcgacgtgc gcgtgacgca
     4021 cgagcgcgac cttcgggtcg taatttaaga accattccaa aagattcgcg agcgaattga
     4081 cgttgatgtc gccgctcgga tttttttcga gtttcggcgg gaaattttcc gtttgtgctt
     4141 gtgccataaa aagattttaa cgcaaaaccg cccgccaaag cgaagccgcg aaaattttcg
     4201 cgctttctga atatcaggaa gaacaggcaa acggctgacc gccgagaaac ggcagataag
     4261 cgttgctgag actgtgctga gcgcccgcct gctcgaacca ttgctcgagc gggtcggtcg
     4321 cggtcacctt gaaaagtttt cccgcctgat tcaactccac gctgccgctc gcgccgttgg
     4381 tctgtgcgta aacggagttg ttatatacct cggaggcaat gacggcggcg tccagagcaa
     4441 taatcccttc ctgcagcgtg acggtatacc cgtcgtaggt cgtgctgatc gagcttgctt
     4501 ccggcgcggg cgaatacaga tttcccatat cggcgatgga agccgaagcc atcggcacga
     4561 tgtcctgcga gttgtaataa cgccacgagt tcgggaaagt cgcgtcaaat gcggcggcaa
     4621 acgcctgatt gccgaccgcc ggcgcggcaa aggtcataac cgccatatca ggaacctgct
     4681 tctgggctga tttaatctgg tagtatagcc acggcgcaaa aaccgtcgat aaaccggcgc
     4741 cgagactgtg tccggtaacc agaatgggta tattattctc gaccgcgtgt cccatcaaaa
     4801 aatcaagcat acgaaccggc gtgcccgcgc tcacggtgct gaccagactc gtcaaatccg
     4861 aaagcccgtc ggatgcgccg cgcgaaatca tcggatactg gtcgctggtc ggataagtcc
     4921 aggcggattg atataaaaca ttaaaatcct gctcgaacca atcttcaaac gcctgccagc
     4981 tgaaatccag caccgagccg cgaatcgcga ccgcatattg ctgcacgtgc tggttgtaag
     5041 cgataaaggc gtaattgccg ttaatctcga cgtccggtat ccaggcaagc gcccaacccg
     5101 gcaggctgga cgcgagactt ttgccgatac tgtcggcgcc gtcgcaatag gcaatcgccg
     5161 aaagcgtcgc cgccgttatc gccccgttat cgccgttgtt atcttcacct attctatgac
     5221 tcattttaat ctccttataa tttaaagtgt cctttccgga tgtcaggaca tcgaccggaa
     5281 tgagccacga ataaaacgag taacccgaat aaaatcaaat aaaatctttt tcttatcggt
     5341 gtttatctcc ggtttatctg tggactgttc gtttctaaaa aaacgctttt tacaaccggc
     5401 gtttctttcc cgaaatctgc ccgcgcgcgg acagttttga ttttgctcaa aatcaaatcc
     5461 tgaaactcgc ttcgactctg ccgaccgtat cgccgcggcg gtttttgacg ggcaagggcg
     5521 acaaatccgc gagagctttc gcgctaaaaa ttccgagctg tcgaaaaagc tcgattgcca
     5581 ccaccgggcg cgcgcgctta tcttcgccgt cctcgatttt aaaagcgatt cccaaacctt
     5641 ttttccattg cggcgaaggc agaacgcccg ccgcgtaaac gccgtcggcg ccgattttcg
     5701 agattaaccg gcgatttgct tcgagcatca aaagcgtgtc gagccgtttg cgcccgccga
     5761 tcatttccgg ataggaaatc atcgccgaaa caatccggcg gcaagcctcg cgcgtttcgg
     5821 cgtcgaaatc cgcgggcggc aaaaccagcc gcgcgaaact ccgcgccatc gccgcgacgg
     5881 gcagagcgaa attcggcgcg gcgcagccgt ccacgccgag ctttatttca tctttcgcca
     5941 cgccggtaaa ctttgaaacg acttccagaa cccgttgctg aaccggatgt tcgaaatatt
     6001 cataagtcgc caaatccgcg ccgatatgct tggcgagcgc gagcatcgcc gcgtgcttgc
     6061 ccgaacaatt attgtgcagc tgcgtcgttt tttcgccgtt gcggagaatt tcctcggcgc
     6121 ggttttcgtc aaacggcgga tgcgccccgc agcgcaaatc cgcttcgctc aagcctatgc
     6181 gagcgagcat tttccgcgac gtttcgacgt gatatttttc tccggaatgc gagccgcacg
     6241 ccagggcgat ttcgttttcg agaaatccga accgctcagc cgcgccgctc gtcaaaaaag
     6301 gaaacgcctg aaaaggcttc gccgacgagc gaaaaaacgt gacggttgcc ggatttccca
     6361 ggctaaaaag cgtttcgccc gcgccgtcaa cgacaatcaa atgcccgcga tgaatcgact
     6421 cgaccgtttc gccgcgaatg actttcgcca ggatttcaga tttcataaag aaagtgaaac
     6481 tacagattta cacaaattaa cgcggatttt aacaggatgg acaggatacg caggataaaa
     6541 agaacttcag cgttattagt tttaaattat cctgtctatc ctgtccatcc tgttcgaatt
     6601 taccttgatg cgctcaacat ttctttggcg tggcggcgcg ccgtttccgt gatttccttg
     6661 ccggtcagca tgcgggcgat ttcctcgact ttttccgttt tgctcaattc tttaatgcgg
     6721 atttccgtgc gatttttcgc cgcctgcttt tcgaccagaa aatgatggtc ggctaaggac
     6781 gcgacctgcg gctgatgcgt gacgcataaa acctgttgag ttttcgccag ctctttcaat
     6841 ttcagaccga cggcttcggc gacgcgaccg ccgatgccga cgtcaatctc gtcgaaaacc
     6901 atcgcctttt cgcgctcgga aagcttcgcc gtcgttttta aaatcagcat caggcgcgag
     6961 gcttcgccgc cggaagcgac ttttgcaatc ggcttgacgc tttcgccgac gtttgccgaa
     7021 aaataaaatt cgatttggtc aaagcctttc gccgtgaaag atttgtcggt ctctgccgcc
     7081 tgcaaattgt tcggcgcgtc gatgcgcact tcgaaacgcg ctttttccag cgcgaccgct
     7141 ttcagattcg cttcgacgtc tttctcgaat ttccgggcgg ctttcgagcg cttttcgttc
     7201 agctcgacgg cgatttgcag atattcttcg cgcgattttt tcagtttttt gcgcagttct
     7261 tcctcgcgca gctcggcggt ctcgatgttg gaaaggcgcg cttccgactc ggaaagatgc
     7321 gccagaacgc tttcgacgct gccgccgtat ttgcgtttca gacgcgagat ttccgccagc
     7381 cggttttcga tttcctccag tctttcgggc gaaaattcca gacgattgcg aaaatccctg
     7441 accgacatcg ccaaatcttc gagaaccgcc tgcgccgtct gcaagccttc catataggcg
     7501 gcaaagctcg attcgtattc ggcgagctcg gtgacgcggc gaatcgcttt ttccagggtc
     7561 gtgacggtcg cttcctcgtt ttcgtaaagc aagctgtagc tttcgtcgct taaagtcgac
     7621 agcttttcca cgttgttcaa acgccgcttt tcctcttcga gcgcgtcgtc ttcgcccgtt
     7681 ttcagattgg ctttgccgat ttcctcgacc tgaaaacaca ggatgtcgag aagctgaagc
     7741 ttctgcgctt cgtcctcgcg caaatttcgc aattctctct gaatcgcggc aagctcgcgg
     7801 tatttttcgg agacttttcc gcgcaggctt tccagattgg cgtattcgtc gagcatttcc
     7861 aagtgagtcg ccggattaaa aagcgtcgcc tgctcgccct gcccgtgaat gtccactaaa
     7921 aatgcgccga ttcgtttcag aaaactttgc gtcacaagct gattattgac aaaaatccga
     7981 ttttttccgg tcgccgaaag ctcgcggcgc acgattaatt cgacttcgcc cccggcgtca
     8041 aactcgacgc cgctttcgtc aaagatttta tttaattcgt cgctcgcggc aatctgaaaa
     8101 agcccttcaa tctgcgcgtt ctgcgtgcct tcctttatca aatccgacga aacgcgctcg
     8161 cccgtcagcg cgcccaggct atcgacaata atggattttc ccgagccggt ctcgcccgtc
     8221 agcagattta agccgcgggc aaactctagc tcgagctcgt caattaaggc gatatttttg
     8281 attttgagaa gactcaacat atttgaaatt gattataaga aaatttcaac acaaagccgc
     8341 aaggaactca aagaagaatt agccgcagag ggcacagaaa acacagagag aaaaatttaa
     8401 ccacagattt acacagatga acacgtgata aaaaaagatt ttatcctgct ttttctttcc
     8461 attctgtttt aatcatctgg gttaatctgt gtaaatctgt agtttcactt cctctctgtg
     8521 ttctctgtgc cctctgcggc taatcttctt aattcattcg aggcacgacc ttttcgcctt
     8581 tcagccattt gccgtttttg taaatcgcgt cgggcggaaa gcgcgtttcc aaatccgcgg
     8641 ctttccagtc aatctggcgc gattttttat agaaaagaaa atccgccgga atgccgacgc
     8701 gccacgcgcc gcttttcggc acgagataaa aattttcgac gttgtattct tccaagccgt
     8761 aagcgccgat ggacatatcg cccagctcgc gcacggtcag cgtcgtttct tcgcccgcgc
     8821 ggcgacggtc gaaagccgtg ccgaggtcgg acataaaagt tctgccgttt tcggataaat
     8881 tgacgacggt cgctttgcgg aaaaggacgt tgtaaagatt gccgacgtcg ttgaaaggca
     8941 aagtgtcgta aacgctcatt tccgccttcg tttcgctgcc cgtgacgatt gagcgcagcg
     9001 cggcgagata ggcgacgaaa tttattttga ccagaaacat ctcgtctttg ccgtagagcg
     9061 cgccggtttc aatcagaatc gcgggcgttc cccaggcgga aaaattatcg ccgaaagcgc
     9121 gcgcgttgta tgcgtcgtcg tagcgcccga cgccgccttt gataaaatga ctcaaagcct
     9181 cgaccattac cgcgcagatg cggcggctgc gctggtgttt ttcgtcggat tttccttccg
     9241 gattgccgct caccgcgagc agcgaaatca ccgcctgacg gttcgtgttt ccggcggcgg
     9301 tcaaaggatt ttgattgtgc aggttgaaac cgatttgcgg ctgccattcg tcgcgcagtt
     9361 ttttcaaaag cctgccttcc ggcgtttgca gggcgcgggc gtcgcggttg atgtcgattg
     9421 actgcaaatt tcgtcgctgg aaaagctcag cgccgtccgg attgagcatc ggcacggcgc
     9481 gaatcgtcaa tttttcctcg atttccctga cccatttttt gtcgcggttc gtctgcaaaa
     9541 acgcgagcat atcgatgagc gcgggagtcg ccgtcggctc gtcgccgtgc atctgcgacc
     9601 acataaaaac gcgcgtcgcg cctttgccga actcgatttg ataaatctct cgattggcgt
     9661 agctgcgccc gacttcttcg acttttaagc cgagcgcgcg cagcttttcc agataattct
     9721 gtaaatcctt atggcgcacc tgcgaaggcg gcaaagtcga aatgtgctgt ttgtcccaga
     9781 ttttgccgaa atcctgcgcc gattgcgccc gcatttcgat atgtagaaaa agcgccagaa
     9841 gcgttaaaag gaaaaatgaa agcggtttca tatcaaacaa gataaactgt ttcttatatt
     9901 tacatcaact ctattttcga tttcaaacgc gaaattttct tcttcccagg cgtttcgagc
     9961 ttgtttataa gttctttcgc cggaggaaat ttttgccgcc ggaatcaggc gatatttcgg
    10021 ctgcgcgttg acagccggaa aaatagaaca catatccgta aagccaaaaa acaaagggaa
    10081 aaaggcgaaa taaacttcct cgctattgtc ttcggcggct tcggactttt atttttccgt
    10141 gtaataaact tttaaatccc tctcgcccgt aatgcgccag tcgccgtcga ttttcgtcaa
    10201 ctgcagctgc gattgagcct tgcctttgtt tcgattttcc gcgccttcga attcccattc
    10261 cttgtcgaaa acggcagttg ctttttcgcc gtcggcgtcg ggcgtcacgc gcagattgct
    10321 gatttccatt cgaaaatcgt cgaagctgcc gaaagctctc tgcttgtcgc tgcgaaccgc
    10381 gccgaggctg acgtcgcgcg cagtgtagta ataatccaga cggtcggcgt aattgctcat
    10441 atgcgcgctg aggtcgccgg cttcgagcga ctgcgcccaa ccgttgacct tctcggaaac
    10501 atcgcttttt atctgcccgg cgtcgatatt cggcgtcggc gaagaatttg catttgcatt
    10561 tgtatttgcc ggcgaaggcg tcggcgaagc gttggcgttg cccttgttcg cattcgcgtt
    10621 cgaagaattt ttgtttgccg gtttgggcga attattgacg cttgaattga ttgccacctc
    10681 ggctccgcga tttcgcaaaa gataccacgc gccgatgccg gcgccgaaaa gcaggagcgt
    10741 cgtcagcgcc gtcaaaaaaa tcacgagccc ggtgcgggat ttttttgcgg ccggcggcgg
    10801 cgtatcggcg gacggcaatt gattttgctg ggtgctgaca gtcggtcgga cgacggtcgg
    10861 cgcttcggta tctttccagc cgatggtaat cggggtttcc gaagcgttaa ccagcacggt
    10921 gccgtcctgc aggcaaaact gcaatgtgtc gtcggtataa gtcgattgac agttcggaca
    10981 atatttcata gcaattcaag gctccgcgcg aaaaatgcgc gataagagtc gattgatttc
    11041 tcgttttcca ctttacattc tgcattatca aacgcccgga atccagcatt aacttccagc
    11101 ggcgaaagaa aatcactcta aacttcgggc gggcgatttt tgcggcatcg ccgaaataat
    11161 aaattaaagc ggcggaatag tcaccgggat tttatcgcct tgattgtgcg cgcggcactt
    11221 tttcatttat acttttgcag aaattatagt ttaggaaaac aagatgaaat ttaaaatcag
    11281 ccagaaaatt attgcactaa gctttgttat tacattcgcg tttttagttt tttcctgcca
    11341 gccgtcacag cagcagcagg tcggcagcgc gcaaacgccg accgaagcct acaagatgct
    11401 ctacgcggcg gtgaaagcta aggacacgga aaaaattaaa aaaatgatgt cgaaagacac
    11461 tatgattctt gccgaaggcg ctgccaaaca gcaaaataaa acgctcgaaa gcgtgctgga
    11521 aaacggcttt tacgcttcga ccttttccgc gactctgccg aaaatgcgcg acgagcgcat
    11581 caaggagaat tacggcgcgg tcgaggtttg gaacgaaaaa gagcgcctct gggaagatgt
    11641 ggcgtttata aaggaagagg acggctggaa agtggcggtc ggcgacatct ttaaaggaac
    11701 ttaccaggtg cccggcaaat cgcagtcaat ccgcgagcgc gaagacgcca acacgatgaa
    11761 tccgaataat gccgtgacgc aggcgaacat caacaccggc gtcaacacaa acgcaattcc
    11821 cgtgaccaac gtcaaggttc cgccgccgcc gctcgcaaat aaagacgacg gagcgaagaa
    11881 aaaatgattt ccgtcgagca gattctggaa attatatcgc aatacaaaaa acacggctgg
    11941 actttacgcc gtgttttgct ttctgacgaa ctgcgcgtgc gggcgtataa cgagctcgtc
    12001 gcgctgtcga cgggcggcgc ggtcgagttt gtttcgtcgg aggtcgaagc cgtatggttt
    12061 tcgcgccctg cgcaagcagc gggcgaggcg tgggagctgc ggcatctttc cagcacgccg
    12121 ttcgctttgt ttgaagtttt tgacgcggac gacgaggaag aagagcgcga tgaagcgcga
    12181 aaagagatgg aaaccagact gaagctttca gtcatcaaat aaacattgtc tttttcctct
    12241 ctagcaattt gcgaaaaggc atttacaaat tctcatattt gttagaaaat tagactgttg
    12301 aaatgatgat taaagatttt ggggcgcgag tcgaaaaagc ctgggaaggc ttgcgaccca
    12361 tgacaaaaaa aatgctggtc ggcgcaatcg aagccaacgc cgccaatctt ccgaaacaaa
    12421 aattttccta cgacgctcac gccgattggg aacttagccg tctgctgacc gcgctggacg
    12481 agcaggcaag cgatacggaa atccgcaagg atgcggaaaa gctacgcgaa atcaaacaat
    12541 tagcggaaac ctgtattaat gttctacaga cgcagaccga atctgccgaa gtttttatac
    12601 agctcgccga acgcgctctg aagaaaaacg attttcgcaa aatcgacgtg ctggctgaca
    12661 ttctgagcca tagattttcg gtcggcgaga tgtgtgaaat cgttcgccag acgacgaacc
    12721 cgggcattcg cgcgctcggt ttcgaggcgc tcgcgctcgt gccggtctcg gcgctgatgc
    12781 agatgctcga cgacccggtt tattttgaaa tcgtccgcaa cgtgctcgaa cagcaagcct
    12841 ttgaatacga atccgaagaa gcccgccgcg ttttggagca gctggatttc gaggaaatga
    12901 acggcgaata aaaaacagaa accggaaaag tgaaagagag aaaaagccgg cttaataatt
    12961 gtcaaccagc tttttcactt tttcactctt tcactttttc actttgccaa taatctgccg
    13021 gaaagccaga attgcccgtt cagataaccg ccgacgctcg gctcggcggc aacgagcttc
    13081 gcgtcggcga cgacgtcaat ttctccgccc agagtatcga ccagaaacca gtaaaatttt
    13141 tcgcccgtca gctcgtttgt cttcaattca aattccttga tttcgcccgt aaaaacgccg
    13201 aaagcgagcg gcggctgcgt gttgtctata tggaaattaa taagcccggt cggaacgaat
    13261 gatttggcgg caaattttaa ttcggaatcc tgttgagcgt aaaaatcccg ctcgctcgca
    13321 aaaacctgaa attcattcga ggcaaaagcc gtcagttgca cctgccggtt ttgcgggaaa
    13381 ttcaaatcgg tattgacgcg aaaatcaggc acgtcgaaaa cgaaaggata ctcgcccgat
    13441 tccggattat tttcttcgga cggatttgcc cacgcgtgaa acgcgccgtc cagctcggaa
    13501 gattcttttt caatcgtttc ggtcaaacaa accgtgcggc ggctttttcc ttcaaaatgc
    13561 gggttaaagc cgatcagttc ctgcgaggca ttcgcctgaa gataaatcgc cgcgcccgaa
    13621 gcgtcggcaa atttgtaata agagccgcgc gggcaaggaa tttcgctgac gtttttgagg
    13681 actttcatta tcacgtcgtt cacgtcctgc tcgccgcgaa cgggaaagcc gatatcggat
    13741 aaattgctca taaattaata aaaagactta gccgcagagg gcgcagagaa tacagaggga
    13801 atttgaaatc acggatttac acagattaat taacagggat aaaaggataa accagggata
    13861 agaaagataa ttttatttat ccctatctat cctgtcatcc ctgttaattt cttttcttat
    13921 ccctgtttat ccgtggtttc attttctcaa aaatctccgc gcctgctgcg gctgattttc
    13981 ctagctcgcc gaaattgatt cgattttcgc ttccttatag cgattcggat acatcgggaa
    14041 acgcatcgtt aattcggcaa cttcgcgttt tactcgccgg cgaacttctt cggattccgc
    14101 ctcgtgaatc accgaggcaa tcagcccggc aatttgtttc atctcggctt ctttcattcc
    14161 gcgagtggtc agcgcgggcg ttcccagacg aatgccggag gcgacgaacg gtttgttcgg
    14221 gtcaaacgga atcgtgtttt tgttgaccgt gatgtggacc tcatccagag ccttttccgc
    14281 cgcttttccg gtaattccct tgccgcccat aaaaacgtcg acgagcatca gatgattgtc
    14341 cgtgccgccg gaaacgatgc gcaaaccttg attctgcaat tcggcggaga gcgcctgcgc
    14401 atttgccaga acctgctgct ggtaggtttt gaaatcttcc ttcaacgcct cgccgaaagc
    14461 gacggctttg gcggcgatga tgtgaaccag aggtccgccc tgcacgcccg gaaacaatct
    14521 tttattgaca tcgccggcgt attcttcgcg gcacaaaatc aatccgccgc gcggaccgcg
    14581 cagggtttta tgcgtggtcg tcgtcacata ttcggcgtgc ggaacgggcg aaggatgaag
    14641 cccgaccgcc accagacccg caatgtgcgc aatgtcagcc atcacgcgcg cgccgattga
    14701 tttggcgatc tcgccgattc gctggaaatc aatcgtccgc ggataggcgg acgcgccgca
    14761 gataatcaat ttcggcttgt tttcttcggc gatgcgctgc aattcgtcgt aatcgatcgt
    14821 ctcggtttcg cggctgactc cgtaatcggc gaccttgtag ttcaagccgg aaaaatttaa
    14881 aggatgcccg tgcgtcagat gcccgccgtg cgaaagattc attcctaaga tttggtcgcc
    14941 gtgttcgagc gcggcgagca gcacggtcat attcgcctgt gcgcccgaat gcggctgaac
    15001 attggcgtgg tctgcgccga aaagctgttt ggcgcgctca atcgccgtgt tttcgacgac
    15061 gtcggcaaac tcgcagccgc cgtaataacg ctttgcggga taaccttcgg cgtatttgtt
    15121 cgtaaaaacc gaacccatcg cttcgagcac ggcttccgaa acgaaatttt ccgaggcaat
    15181 caattccaag ccgtcggttt ggcggcgcac ttcgctgtca atcgcttccg agataatcgg
    15241 gtcggctgcc gctaaatctg ctgtaaaaaa attgttcatc gtgtttccta taatttttca
    15301 atttggtcta attgtatttt ttccgcgctt ttgttttttg aattgaaa
//
LOCUS       DASWNE010001063         7620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_141042, whole genome shotgun sequence.
ACCESSION   DASWNE010001063 DASWNE010000000
VERSION     DASWNE010001063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7620
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141042"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1427
                     /locus_tag="VGB68_20265"
     CDS             <1..1427
                     /locus_tag="VGB68_20265"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY0051644.1"
                     /translation="FKNAGSEINAEVCVVGGGPAGATIARKLALLGHRVCLIEKTDNS
                     QNKVGESLPPNILVLLDALGVREKIEAASFLRPDKALVRWSDESAFTKSSHGQPGFLV
                     NRGRFDRILLESAREAGVDVIRRAQALSPVFDEARQWNIPVRRDSGWLKIKAKYLVEA
                     AGKRSFFKQKRKRYSAATVALCAYWRNANVESRATLVEAGREEWFWGATLPDGDFSAM
                     VFIDAKRRRGKRDLESFYRSLLAESTLLRGCLSGTLSGQVKGWDASGYIDEEPVNENL
                     IKVGEACFSIDPLASQGVQAAMNSGLQGGFVVHTLLTRPENSAEAMQFYRNRQEETVA
                     RHQNLAARFYAESYFHNTGAFWRERSILKTAGQAIIPPVNPALSRELYLKLSNAATLI
                     DTPCIADDIITKTRALTYDGLERPIAYVNNTAIALLLDSIPHGGTIAEITQAWLPYIS
                     TQESTRIINWLYDAGILKPSAATK"
     gene            1761..2954
                     /locus_tag="VGB68_20270"
     CDS             1761..2954
                     /locus_tag="VGB68_20270"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEY0051645.1"
                     /translation="MKKIYLHRAKNQIARPNTIRGINKQIVLNYVRDRAPISRAEIAR
                     ETALQRSTVSAIVDDLHDAGLIEEIGTGDSTGGRKPTLLKIRTGVPVAIGIDITPRLT
                     TIATADLSGQILEKETFPTSPDADGMSEQIVAKVTKYAKLHNKYDLEVGISVPGIADQ
                     ASGNVVYIPYFQWSNWDITRQITEQTGLPVIVDNDANAVALAELWFGEEKIRKHRNFI
                     TVLVADGIGTGIIFDGQVYRGEKGAAGEFGHMIVGDNAPVQCSCGSRVCWEAHASEKA
                     SVYRYQMSNGTTKNSDINFEQLIKLAHEGKPHATKALKESGKYLGIGISNLIVGFSPQ
                     AVVISGNIVKAWALIKDEIQSLAERSVRQGLGRPTITASSLGDSPTLIGSLSLVLARK
                     FASAN"
     gene            3221..6562
                     /locus_tag="VGB68_20275"
     CDS             3221..6562
                     /locus_tag="VGB68_20275"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY0051646.1"
                     /translation="MTTIITAQTESARVQGTVTDTSGAAIAGATVTVRDLGTSREVSA
                     QTNEEGNYSILALQPGRYQITVTQSNFKTTRQEVTLEVAQNATLSFSLEPGAVTETVT
                     ITSDTPLVDSTSSSIGEVIQGRQIVELPLNGRNVLELARLTPGVTQGVVGGFASGAGG
                     DAETYRGGNTGGAALSVNGQRTQANNFLLDGVDNNESLVNTINIFPSADAVQEFRVQT
                     SVATAEFGRGGGAIINSVIKSGQNDFHGSSYLFIRNSAFDARPAFYDSVQNQRRTPLF
                     RRSQFGGTIGGPVIIPGFGEGTPSIHNLKDKVFFFFSYDGLRQFLPRATETATVPTAA
                     FRNGDFSQLLNPASSGLGNAIQLRDPLTGANILNNRLDLLPPGRLNQAALAYLRAFPL
                     PNNGTQVQGNFINTRNQIDDQDVFDLRIDANLNDKNQMFVRTSYGRYNQTVESRLPTL
                     PAGFGSGSNPTRTKAVVVGFNTSITPTLFNELRLQANRLRYGYEPPFGDQPISANLGI
                     VNANRDSTLGGGALIGGYNGQLEYTGDYGPYRVPQNTYQLVDSLSYVTGNHTFKFGGT
                     LLKRNVQLFRPLAGKGDFRMYGNGEFNQCPNGGAAPNEAASGTTRFEQADLLIGFFCT
                     YDIGFQNGLVGTTNWENGVFAQDDWRVTSKLTLNLGLRYEYFTNPAEEYARQANFDLP
                     TGRLILAADEKDTLTRTDKNNFSPRLGLAYDLTGKGKSVIRGGYGIFYFLDRGGINNQ
                     LAQNPPYSGSARYSYNEGYRITFSGQGPLNNNNNTLATNPLPLPGLNTSPAFLNNPVG
                     SNVLAIVPDNKISNVQQFNVQFQQQLSGDTSVSIGYVGTRGRNLILYYNLNGNALTND
                     SSVPCPIAGRTRGNCYPGLGSVNVRDDNGKSQYDSLQVQLERRFSRGWQYIAAYTFSK
                     TKDNGEGAFDSTAGGINYIQPFATSRLDYPHVFSFATVYELPYGRGRQFGSDIPKALD
                     LLIGGWQINSIFRAQSGNPFDVRVNNALVNLVGDPYNGSNGQFLNRSAFSPAATGIGN
                     LERNSLRSPSTYQLNFGISKNFNFTERAKLQVRAEAFNLTNKTQLGTPNTDYNNTSTF
                     NGFGTIRGTSPYSNRQLQFGLRLEF"
     gene            6674..>7620
                     /locus_tag="VGB68_20280"
     CDS             6674..>7620
                     /locus_tag="VGB68_20280"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY0051647.1"
                     /translation="MKKILRLILIIGIAGFACPAAQAQNQAPLVEKVEPPSWWAKHTI
                     NPVRLLVRGKNFQNAKVASKNNFFKISNTKTNERADYLFFDVTISANTKAGKYDFEVS
                     AAGGKTIVPFEILAPLDTETNFQGVTNKDVIYLIMTDRFSDGDASNNNPKNAPQSANG
                     RRNPRAWHGGDFQGVIDKLPYLKELGVTAIWLTPWYDNPNQANECDKPWCPYTNYHGY
                     HAIDYYSTEDHFGALADLQKMVREAHKQGIKVIQDQVANHVGVQHVWAKSPPLDNWFS
                     RFSQNSFNNSVLLSPNASESERDNLLKGWFNEFLPDLNQ"
BASE COUNT         1939 a         1916 c         1996 g         1769 t
ORIGIN      
        1 gattcaaaaa cgccggttct gaaataaacg ccgaggtctg cgtcgtcggc ggcggacccg
       61 ccggagcgac gattgcgcgc aagctcgcgc ttttaggtca cagagtctgt ttaatagaaa
      121 aaacggataa ctcgcaaaat aaagtcggcg aatccctgcc gccgaatatc ctggttttac
      181 tcgatgcgct cggtgtcaga gaaaaaatcg aagccgcgtc gtttcttcga cccgataaag
      241 ctctcgttag atggtctgac gaatcggctt tcacaaaatc ttctcacgga cagcccggat
      301 ttctggtcaa tcgcgggcgt tttgaccgga ttttgcttga gtccgccaga gaggcgggcg
      361 tcgatgtgat tcggcgcgcg caggcgctga gcccggtttt tgacgaggcg cggcagtgga
      421 atattccggt tcgccgcgat tcgggctggt taaaaatcaa agcgaaatat ctggtcgaag
      481 ccgccggcaa gcgttctttc tttaaacaaa agcgaaaacg ttattcggcg gcgaccgtcg
      541 cgctgtgcgc ttactggcgg aacgcgaacg ttgaaagccg ggcgacatta gtcgaagccg
      601 gtcgggaaga atggttttgg ggcgcgacat tgccggacgg cgattttagc gcgatggtat
      661 ttatcgacgc taaacgccgg cgcggtaaac gtgatttgga atctttctat cgctcgctgc
      721 tcgccgaatc gacgcttttg cgcggctgtc tgagcgggac gctcagcggt caggtgaaag
      781 gctgggacgc ttcgggctat atcgacgaag aacccgttaa tgaaaattta ataaaagtcg
      841 gcgaagcctg tttttcaatc gacccgctcg cttcgcaggg cgtgcaggcg gctatgaatt
      901 ccggtctgca gggcggtttc gtcgtccaca cattattaac ccgaccggaa aattcggcgg
      961 aagcgatgca gttttaccga aaccggcagg aagaaaccgt cgcgcggcat cagaatctgg
     1021 cggcacgatt ctatgccgaa tcttattttc ataacacagg cgcgttctgg cgagagcgct
     1081 caatcctgaa aacagccggg caagctataa ttccgccggt aaatccggct ctctccaggg
     1141 agctctacct gaaattgtca aacgctgcaa ctttaataga tacgccctgt attgccgacg
     1201 acattattac aaaaacgcgg gcgctgacat acgacggtct tgagcgcccg atcgcttatg
     1261 tcaacaatac ggcgatcgct ttattattgg attcgattcc gcacggcggc acgatcgccg
     1321 agataacgca ggcgtggctg ccttatattt caacgcagga aagcacaaga ataattaact
     1381 ggctttacga cgcgggtatt ctcaagcctt cggcagcgac gaaataaaag ccttatcggc
     1441 aaaaaagata gcagagcaga tttgcgcggc taatatcgca aagccgtaaa ggcggaaacg
     1501 cgaatctgaa agacatcatc gatatctctg tcaccggaaa aattaatcga catttttgtg
     1561 acgcgggaaa ttaaccggat tttgctgaat ttaaattagt taaaattact aatcgatatc
     1621 gccgcttcaa tctctatgca gaaattttta aaaatgttgt tgaaaaaaaa atgaaaaagg
     1681 tttatgaata gtttgttcgt ttaaacaaca aactaagtgt agccattcag aaaacctatt
     1741 tgccccaaat ttttgcgact atgaaaaaaa tttacctgca tagggctaaa aaccagattg
     1801 cccgcccgaa cacgattcgc ggaattaata aacaaatcgt tttaaattac gttcgcgacc
     1861 gcgcgccgat ttcgcgggcg gaaatcgctc gtgagaccgc gctgcaaagg tcgaccgtct
     1921 cggcaattgt tgacgattta cacgatgccg gtttaatcga agaaatcggg acgggcgatt
     1981 cgacgggcgg aagaaaaccc actttgctga aaatcagaac cggcgttccg gtcgccatcg
     2041 gcatcgatat tactcctcgc ctgacaacga ttgcgacggc tgatttgtcg ggtcagattc
     2101 tggaaaaaga aacttttccg acctcgccgg acgccgacgg gatgagcgaa cagattgttg
     2161 ccaaagtgac gaaatacgcc aagcttcaca ataaatatga tttggaggtc ggcatcagcg
     2221 tgcccggaat tgccgaccag gcgagcggaa atgtggttta tatcccgtat tttcaatgga
     2281 gtaactggga tataacccga caaataaccg aacagaccgg attgccggta attgtcgata
     2341 acgacgcgaa cgccgtcgcg ctggcggaat tgtggttcgg cgaggaaaaa atcagaaaac
     2401 accgcaattt tattacggtt ttagtcgccg acggaatcgg aacgggcatt attttcgacg
     2461 gtcaggtcta tcgcggcgag aaaggcgcgg cgggcgaatt cgggcatatg atcgtcggcg
     2521 acaatgcgcc cgtccagtgt tcgtgcggaa gccgcgtctg ctgggaagcg cacgcttcgg
     2581 agaaagcgtc ggtttaccgt tatcaaatga gcaacggaac gaccaaaaac agcgacataa
     2641 atttcgaaca gctaatcaaa ctggcgcacg aagggaaacc tcacgccacc aaggctttaa
     2701 aggaaagcgg caagtatctg ggcatcggca tctcgaatct gattgtcggc ttcagcccgc
     2761 aagccgtcgt catcagcggc aatatcgtta aagcgtgggc gttaatcaaa gacgagattc
     2821 aatcgctggc tgaaagaagc gtccggcagg gcttgggcag accgacgatt acggcgtctt
     2881 cgctcggcga cagtccgacg ctgatcggct cgttgagcct ggttctagcc agaaaattcg
     2941 cttcggcaaa ttaaattcgc tttttgttga taattttgca aataaatctt gacaagtgta
     3001 ttacaaatgt attaaaactt tgtttgtttc cacaacaaac aaaatcttac gttttaaaat
     3061 catattgtta agtcgctgat ttttattaaa taaaaatcgt ttcggagacc tgtaaaagac
     3121 gaggaatctt aaagctaata agagaggaat ttggattatg aataaattga gagcattgag
     3181 tcggcacaga gtgtttggct tattagttgc cattctgtgt ttgacgacaa tcataacggc
     3241 gcaaaccgaa tcggcgagag ttcagggaac ggtcaccgat acctcaggcg cggcaatcgc
     3301 cggagcgacg gtcacggtca gagacttggg aacaagccgg gaagtatctg cacaaacaaa
     3361 cgaagaaggt aactattcca ttctggcgct tcaaccggga cgttaccaaa tcacggttac
     3421 tcaaagtaat tttaagacga ccagacagga agtaactctg gaagtggcgc aaaacgctac
     3481 cttaagtttt tcgcttgagc cgggcgcggt caccgagacg gtgaccatta caagcgacac
     3541 gccgctggtc gattcgactt cctcgtctat cggcgaagtt attcagggac ggcagattgt
     3601 cgaactgccg ctcaacgggc gcaacgtttt ggagctggcg cggctgacgc cgggcgtcac
     3661 gcagggcgtc gtcggcggct ttgcttcagg cgcgggcggc gacgcggaga cgtatcgcgg
     3721 cggcaacacg ggcggcgcgg cgctttccgt taacgggcag cgaacgcagg caaataattt
     3781 tttgctggac ggcgtcgata ataacgaatc gctcgtcaac accatcaaca tctttccgtc
     3841 ggcagacgcc gttcaggaat tccgcgttca gaccagcgtg gcgaccgcgg aattcgggcg
     3901 cggcggcggc gcgattatca acagcgttat caaatccggt cagaatgatt ttcacggcag
     3961 ctcgtacctg tttattcgta acagcgcctt tgatgcccgc ccggcttttt acgattcagt
     4021 ccaaaaccag cgccgaacgc ctcttttccg gcgcagccag tttggcggga caatcggcgg
     4081 accggtcatc attcccggtt tcggcgaagg aacgccgtcg attcataatc tgaaagataa
     4141 agtgttcttt ttcttcagtt atgacggctt acggcagttt ttgccgcgcg cgaccgaaac
     4201 ggcgactgtt ccgacggcgg cttttcgcaa cggagatttc tcgcagttgt taaatccggc
     4261 ttcttctgga ttgggcaacg ccattcaatt gcgcgacccg ctgaccggag cgaacattct
     4321 caacaaccgt ttggatttgc ttccgccggg gcgcttaaat caagcggcgc tggcttattt
     4381 gagggctttt ccgctgccga ataacggaac gcaggtgcag ggtaatttta taaacacgcg
     4441 taaccaaatc gacgaccagg atgttttcga cctgcgcatc gatgccaatc tgaacgataa
     4501 aaatcagatg ttcgttcgca ccagttacgg tcgttataac cagacggtcg aatcgcgcct
     4561 gccgacgctg cccgccggtt tcggttcggg ttcgaatccg acgcgcacga aagccgtcgt
     4621 cgtcggtttt aacacttcga taactccgac gcttttcaac gagctccggc ttcaggcaaa
     4681 tcgtctgcgt tacggttatg agccgccgtt cggcgaccag cctatttcgg caaatctcgg
     4741 aatcgtcaac gccaaccgcg actccacttt aggcggcggc gcattaatcg gcggttataa
     4801 cggtcagctc gaatacacgg gcgattacgg tccgtatcgc gttccgcaaa atacatatca
     4861 gctggtcgat agtctcagct acgtgacggg caatcatacg ttcaaattcg gcggcacgct
     4921 tttgaagcgc aacgtccagt tgtttcgtcc gctggcgggt aaaggcgact tccgaatgta
     4981 tggaaacggc gagtttaatc aatgcccgaa cggcggagcg gcgcccaacg aagcggcttc
     5041 gggaacaaca cggtttgaac aagccgattt gttaatcggt ttcttctgca cttacgacat
     5101 cggcttccaa aacggtctgg tcgggacgac taactgggaa aacggtgttt ttgcgcagga
     5161 cgattggaga gttaccagta aattaactct gaatctcggt cttcgttacg agtattttac
     5221 caatccggcg gaagaatacg cccggcaggc taactttgat ttgccgaccg gacgacttat
     5281 cctggcggct gacgagaaag atactttgac cagaaccgac aaaaacaatt tcagcccgcg
     5341 cctcgggctc gcttacgacc tgaccggcaa aggcaagtcc gttattcgcg gcggttacgg
     5401 gattttctat ttcctcgacc gcggcggcat caataaccag ctcgctcaaa acccgcctta
     5461 ttcgggcagc gcccgataca gctataatga aggttaccgg ataacttttt cgggacaagg
     5521 tccgctcaat aacaacaaca atacgctcgc cacgaatcct ttgccgctgc cgggattaaa
     5581 taccagcccg gcgtttttga acaatccggt cggctcgaat gttctggcta tcgtgccgga
     5641 taataaaatc tcgaacgttc agcagttcaa tgttcagttt cagcagcagt taagcggcga
     5701 cacgtcagtc agcatcggat atgtcggaac tcgcgggcgc aatctgattc tttattacaa
     5761 cctgaatgga aacgcgctga cgaatgattc ttccgtgccc tgcccgattg ccggcagaac
     5821 gcgcggaaat tgctatcccg gattgggcag cgtcaacgtt cgcgacgaca acggcaaatc
     5881 tcagtatgat tcgctgcagg tgcagcttga gcggcgcttt agtcgaggct ggcagtatat
     5941 cgccgcctac actttttcga agacgaaaga caacggcgaa ggcgcgtttg acagcacggc
     6001 gggcggcatc aattatattc agcctttcgc tacttcgagg cttgattacc cgcacgtttt
     6061 ctcttttgcg accgtttatg agcttcccta cgggcgcggc agacaattcg ggtctgacat
     6121 tcccaaggcg ctggatttgc ttatcggcgg ctggcagata aacagcattt tccgcgctca
     6181 gagcggcaat ccgttcgacg ttcgcgtaaa taacgcgctg gtcaatctgg tcggcgaccc
     6241 ttataacgga agcaacggac aatttctgaa tcgcagcgct ttttcgccgg cggcgaccgg
     6301 aatcggtaat ctggaacgca actctctgcg ctcgccttcg acctatcagt taaatttcgg
     6361 tatctccaaa aactttaatt ttaccgaaag agccaagctg caagtccggg ctgaagcgtt
     6421 caacctgacg aataaaactc agctcggaac gccgaatacc gattacaaca ataccagcac
     6481 gtttaacggc ttcggcacga ttcgcgggac ttctccctac agtaatcgcc agcttcaatt
     6541 cggacttcgt ctggagttct aaattcaata ccggagatgc cgtttcgggc aaccggcatc
     6601 ttgttatttc tcagggcgtg gaacagaatg ttttcacgcc ctgtattttt atattgattt
     6661 ttcgagcgga atgatgaaaa agattcttcg tttgattttg attatcggaa tagccggttt
     6721 tgcgtgtccg gcggcgcagg cgcaaaatca ggctccgctg gtagaaaaag tcgagccgcc
     6781 gagctggtgg gcaaaacaca cgataaatcc ggttcgcctg ctggttcgcg gcaaaaattt
     6841 tcaaaacgcg aaggtcgctt ccaaaaacaa tttttttaaa atttccaata caaaaacgaa
     6901 cgagcgcgcc gattatcttt tcttcgacgt gacgatttcg gcgaatacaa aagccggaaa
     6961 atacgatttt gaagtctcgg ctgccggcgg aaaaacgatt gtgccttttg aaatcctcgc
     7021 gccgctcgac accgagacga attttcaggg cgtcacgaac aaagacgtca tctacctgat
     7081 aatgaccgac cgtttctcgg acggcgacgc ttcgaacaac aatccgaaaa acgcgcctca
     7141 atcggcaaac ggtcgccgaa acccgcgcgc gtggcacggc ggcgattttc agggcgtgat
     7201 tgataaattg ccgtatttaa aggaattagg cgtcaccgca atctggctga cgccgtggta
     7261 tgacaatccg aatcaggcga acgaatgcga taagccgtgg tgcccgtaca cgaattatca
     7321 cggttatcac gcaatcgatt attactctac ggaagaccat ttcggcgcgc tggctgattt
     7381 gcagaaaatg gttcgggaag cgcataaaca gggcatcaag gtcattcaag accaagtggc
     7441 aaatcacgtc ggcgtccagc acgtctgggc gaaaagcccg ccgctggata attggttcag
     7501 ccgattttcg caaaactcat ttaataattc ggttctgctt tcgccgaacg cttcggaaag
     7561 cgaaagagat aatttgctga aaggctggtt caacgaattt ctgcccgatt tgaatcagag
//
LOCUS       DASWNE010001064         2496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_176260, whole genome shotgun sequence.
ACCESSION   DASWNE010001064 DASWNE010000000
VERSION     DASWNE010001064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176260"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VGB68_20285"
     CDS             complement(<1..752)
                     /locus_tag="VGB68_20285"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HEY0051648.1"
                     /translation="MRTAKSILQKVYSAFFLLLLCSQVFGTYSADRIRFQQQATFGYS
                     PALDTEISSIGIEQYVNNQLGAAAIPLNPFPDENHPSYAYWLKPTQPPADCNNTDLIG
                     PPDKPDVPATCFRDTYTMHPLQQWFFKEALYGSSQLRHRVAWALSQMWVTSAVEIKQS
                     RHMTEYYKILYNYALGDDNNTGSPALLDDSYFGLMKAMTLSPAMGEYLDMAYSQKTVP
                     NTVIKPNENYPRELLQLFTTGLNEIVVVNNNPT"
     gene            complement(831..1937)
                     /locus_tag="VGB68_20290"
     CDS             complement(831..1937)
                     /locus_tag="VGB68_20290"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEY0051649.1"
                     /translation="MKIPRTESARGSKAACAVFFAFFFLTFAFDAYSIDPTNKLDFDG
                     DGKADIAVYREGSANDRVSSQAYSASYWYYLGSTSGFGSFPWGRTLDVPIPADYAGDS
                     KTDPTIFRWFEPGLSPFVGNTFWISGGGVRGFGNTTGRKMGRNFVLETGGAPQKAEIG
                     EFQHVNIFYDQDSVTPFYQYRLYWQIGSTNLDYIVTNVPSGQSASAYQQVPVPDDYSG
                     DGKSEVAVFNTNTGCYHIWYGNTNDPFTPNCFGSGFTPAPGDYDGDGRVDYGVFKSTG
                     TNSFQWQFINSQTNGTPTTYSLTTGWSGDGKPVAADYDGDGKTDYSVAQSASGNWNWT
                     IIKSGCSSCSPITATFGVNTDTLLASPYFYDPWY"
BASE COUNT          678 a          602 c          577 g          639 t
ORIGIN      
        1 gtcgggttat tattaacgac gacaatctcg ttcaagccgg ttgtaaaaag ctgcaacaac
       61 tcgcgcggat aattttcgtt tggttttata accgtattcg gaaccgtttt ctgactgtaa
      121 gccatatcga gatattcgcc catcgccggg ctaagggtca tagctttcat cagaccgaaa
      181 taagaatcat ccagcaatgc gggagaaccc gtattattat cgtcgccgag cgcatagttg
      241 tacaaaattt tgtaatactc cgtcatatgg cgcgactgtt tgatttcgac tgccgaagtc
      301 acccacatct ggctgagcgc ccaggcgacg cggtggcgca gctgcgacga gccgtaaagc
      361 gcttctttaa agaaccactg ctggagcgga tgcattgtgt aagtatctcg aaaacaggtt
      421 gcaggaacat cgggtttatc gggcggaccg attaaatcgg tattgttgca atctgcgggc
      481 ggctgtgtcg gcttcagcca ataggcatag ctcggatgat tttcgtcggg aaaaggattg
      541 agcggaatag ccgccgcgcc taactgatta ttgacatatt gctcgattcc gattgaggag
      601 atttcagtat caagcgccgg gctatacccg aaagttgcct gctgctgaaa tctgatccgg
      661 tcggctgaat acgttccgaa aacttgtgaa cacagaagta gtaaaaaaaa cgcggaatag
      721 accttctgta aaattgattt tgctgtcctc atattaatct cctgctctgt taaaactgca
      781 ttttgtcggt ggctcgaccc aaaaatcgag aattatcctg caactcgtct ttaataccag
      841 gggtcgtaaa agtatggact tgcgagcaaa gtatcggtgt taactccaaa agtagcagta
      901 atcggactgc agctagaaca accgcttttt ataatcgtcc agttccagtt gccgctggcg
      961 gactgagcga ccgaataatc cgttttgccg tctccgtcat aatccgcggc gacgggtttt
     1021 ccatctccgc tccagcccgt ggtcaacgaa tatgtggtcg gtgttccgtt agtttgcgaa
     1081 tttatgaatt gccattggaa gctattggtt ccggtagatt tgaacacgcc gtagtcaacc
     1141 cgcccatcgc cgtcataatc gcccggcgca ggcgtaaacc cgctgccaaa gcagttcggg
     1201 gtgaaaggat cgttcgtgtt gccgtaccaa atgtgataac agccggtatt cgtattaaat
     1261 acggcgactt cgctttttcc gtcaccgctg taatcgtccg gcaccggaac ctgttgatac
     1321 gccgacgcgc tttgcccgct cgggacattc gtcacaatat aatcgagatt ggtgctgccg
     1381 atctgccagt aaagtcgata ttgataaaac ggcgttaccg aatcctgatc gtaaaaaatg
     1441 ttcacgtgtt gaaattcgcc gatttcggct ttctgcggag cgccgccggt ctcgagcacg
     1501 aaattacgtc ccattttccg cccggtcgta ttgccgaagc ctctgactcc gcctcctgaa
     1561 atccaaaacg tattgccgac aaatggagac agccccggct cgaaccatct gaaaatcgtc
     1621 gggtcggtct tactgtcgcc ggcataatca gccggaatcg gcacatcgag cgttcgcccc
     1681 cagggaaaag agccgaaacc gctcgtactt cccaggtaat accagtaaga agcggaatag
     1741 gcttggcttg ataccctgtc attagcagag ccttcgcggt aaacggcgat atcagccttg
     1801 ccgtcgccgt caaaatctaa cttattagtt ggatctatgg aataagcatc gaatgcgaaa
     1861 gttaaaaaaa agaatgcaaa aaaaaccgcg caggcagctt ttgagccgcg ggcagattcc
     1921 gtcctcggga ttttcattat agccctccta agagcttcgg aagcgacggc gatattttga
     1981 aatacgaccg tcctaaactt taccggaaaa cattttttgt cggattattg gaagatgaaa
     2041 cctgtagaac aaagacaagt tgggcatgga tttcaccaaa tgttttcaat tgtgtattag
     2101 ttgtgtgcaa ttttttagac aaggactgcg aacaattaca attcaagccg ccctttacaa
     2161 aggcaaaatt acaaagaagt ttattgttca cagactttct agctataatt ggcaaaaatt
     2221 aaaaaaggtt tcaccttttt ttacgagaaa cgtatctgtt tcatctcagt aaactattcc
     2281 gcttcaatct gctcgtccgc ttccttgttt cgacgagaag aaaatgctcc ccagacggca
     2341 aaaccgataa acaaaagcgt tccgacgata acgacaatta gcgtgctcaa atccatataa
     2401 atctcctttt attaaaaaac tcgtcctgac aaataccaga ctaaaagaaa taatcattcg
     2461 ccaaaaccgt gtgaacacgc gcgcgcaaaa caagtg
//
LOCUS       DASWNE010001065         2246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_418981, whole genome shotgun sequence.
ACCESSION   DASWNE010001065 DASWNE010000000
VERSION     DASWNE010001065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418981"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VGB68_20295"
     CDS             <1..613
                     /locus_tag="VGB68_20295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051650.1"
                     /translation="RQPVFLLCLVWTIPYLLFLFFFIPGNTFYRLFYFPALILLIGTM
                     LAPFESFRQKRRWRLAALVGALCLYNFLFYIYPNSRVRENTPLALALRANHVWSEKTV
                     VFHAANAAAFDALDTNNRLVKYFNPAVVWKPLNFITLEEFQGEIQAIRAGGGAVWLDA
                     SAIDKLAANPQTARWLADNSRQTELNLEAHRMKYVQITLNSPK"
     gene            718..1329
                     /locus_tag="VGB68_20300"
     CDS             718..1329
                     /locus_tag="VGB68_20300"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEY0051651.1"
                     /translation="MKSFIFTISILLCLAGCARAQTYYGTDDVKIFREGREKDFRNPQ
                     MSPLRPEDVSKFQGLNYFPVDASFRVKAEFTGTPDEKYFMMPTSSGRSRKYIKVGTLS
                     FKLNGKDYVLNAYQSAAIAEVAKMQEAGSSLFVPFRDLTSGKETYGSGRYLYLSTPKD
                     KDMILDFNLAFNPSCAYGDSQFSCPIPPKENFLPIEIKAGEKN"
     gene            1331..>2246
                     /locus_tag="VGB68_20305"
     CDS             1331..>2246
                     /locus_tag="VGB68_20305"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEY0051652.1"
                     /translation="MNTILLLQNLVNPNVNLPIVLPEMIVAVVGIVVMLYDCFAPKQR
                     KVTGAISLVGLALSAAALFTLWTANGGAAQTAWNGMIALDGLRLSFSVVFLLVSALTI
                     LISTVWVEREDVPVGEYHAMLMFATFGMMLMASGNDLVIIFLGLETLSIATYVMAGLR
                     RTDLRSNESAMKYFILGSFASAFLLYGMALIYGATATTNITEIAAKISNPNFPALLLA
                     GAAMLVVGFGFKAATVPFHVWTPDVYEGAPTPVTAFMAAGPKASALASFLRVFVLGFP
                     LVAGIQASQFLHDSWIMALTVMAMLTMTV"
BASE COUNT          482 a          552 c          607 g          605 t
ORIGIN      
        1 tcgccagccg gtatttttgc tttgtctcgt gtggacgatt ccgtatctgc tttttttatt
       61 tttctttatt cccggaaata cgttttacag gctcttttat tttccggcgc tgatactttt
      121 gattggaaca atgctcgcgc cgtttgaaag tttcaggcaa aagcggcgct ggcggctcgc
      181 ggcgctggtc ggcgcgcttt gtctttataa ttttcttttt tatatttacc cgaattccag
      241 agtccgggaa aatacgccgc tcgcgctcgc cttgcgggca aatcacgtct ggtcggagaa
      301 aaccgtcgtc tttcacgcgg caaacgcggc ggcgttcgac gcgctcgaca cgaacaaccg
      361 gctggttaaa tattttaatc cggcggttgt ctggaagcct ttaaatttta taacattgga
      421 ggaatttcag ggcgagattc aggcaatccg ggcgggcggc ggcgcggtct ggctcgacgc
      481 ttcggcgatt gataaactgg cggcgaatcc gcagacggct cggtggctgg cggataattc
      541 gcgtcaaacc gaattaaacc tcgaggcgca tcgtatgaaa tatgttcaaa ttactctaaa
      601 ctctccgaaa taaaaggaca attacgcgtt tcggattgga aagacgggcg atttacaact
      661 aaaatgaatg aataagactt taacccgcgt taagtatcag atttggaaat tcaccggatg
      721 aaatctttta tttttacaat ttcgattttg ctttgtcttg ccggctgcgc ccgcgcgcag
      781 acttattacg gcacggacga cgttaaaatt tttcgcgaag ggcgcgaaaa ggattttcgc
      841 aacccgcaga tgtcgccttt gcgtcccgaa gacgtttcca aatttcaggg tttgaattat
      901 tttcccgtcg atgcaagttt tcgcgtcaaa gcggaattta ccggaacgcc ggacgagaaa
      961 tattttatga tgccgacctc ttcgggaagg tcgcgcaaat acataaaagt cggcacgctg
     1021 agctttaagc tgaacggcaa agattacgtt ctgaacgctt atcaaagcgc ggcgattgcg
     1081 gaagtcgcga aaatgcagga agccggaagc tcgcttttcg ttcctttcag agatttaacg
     1141 agcggcaagg aaacctacgg aagcggcagg tatctttatc tttcgacgcc gaaggataaa
     1201 gatatgattc tggattttaa tctggcgttt aatccttcgt gcgcctacgg agattcgcag
     1261 ttttcgtgcc cgattccgcc gaaggaaaat tttttaccaa tcgagataaa agccggcgag
     1321 aaaaattaaa atgaatacga ttttattact acaaaattta gtcaatccga atgtcaacct
     1381 gccgattgtc ctgcccgaaa tgattgtcgc ggtcgtcggc atcgtcgtga tgctctacga
     1441 ctgtttcgcg ccaaaacagc gcaaggtgac gggcgcgatt tcgctcgtcg ggctggcttt
     1501 gtcggcggcg gctcttttta cgctctggac ggcaaacggc ggcgcggcgc aaaccgcgtg
     1561 gaacggaatg atcgcgctcg acgggctgcg tctcagcttt tcggtcgtct ttctgctggt
     1621 ttcggcgctg acgattctga tttcgaccgt ctgggtcgaa cgcgaagacg ttcccgtcgg
     1681 cgaatatcac gcgatgctga tgtttgcgac cttcgggatg atgctgatgg cttcgggcaa
     1741 cgatctggtc atcatctttc tcggtctgga aacgctttcg attgcgacat acgtgatggc
     1801 tggactcagg cgcacggatt tgcgctcgaa cgaatcggcg atgaaatatt tcatcttagg
     1861 ctcgtttgcc tcggcgtttc tgctttacgg aatggctttg atttacggcg cgacggcgac
     1921 gaccaacatc accgaaatcg ccgccaaaat ctcgaatccg aacttccccg ctcttttact
     1981 ggcgggcgcg gcgatgttgg tcgtcggctt cggttttaaa gccgcgaccg tcccgtttca
     2041 cgtctggacg ccggacgttt acgaaggcgc gccgacgccc gtgacggcgt ttatggcggc
     2101 gggaccgaag gcttcggcgc tggcttcatt tctgcgcgtt ttcgttctcg gctttccgct
     2161 ggtcgccgga attcaggcgt cgcagttttt gcacgactcc tggattatgg ctttgaccgt
     2221 gatggcgatg ctgacgatga ccgtcg
//
LOCUS       DASWNE010001066        10562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_176367, whole genome shotgun sequence.
ACCESSION   DASWNE010001066 DASWNE010000000
VERSION     DASWNE010001066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10562
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176367"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VGB68_20310"
     CDS             <1..249
                     /locus_tag="VGB68_20310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051653.1"
                     /translation="FSSIKPAIYPVRKFAAHVHMTITGKNFREDWIDSILFEDDRLIS
                     AQERNQAGKRGGFNPILKLEKGSDGIFRAARDIQLWKA"
     gene            262..1242
                     /locus_tag="VGB68_20315"
     CDS             262..1242
                     /locus_tag="VGB68_20315"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051654.1"
                     /translation="MRKLISFLFIFVCFCAPASACHSNKNSYVWNEMTGSAAYPQSYG
                     FPVFAANEKMFAFHYEAVWNSADGANWTKTDLPSVKRDAYETRYVQFNNAVYALGQNR
                     GNYAVGIRFGSTIRRTTDFKTWETLAEKSSLPDRVFHGTIVFGGKIWLMGGYDGKNYY
                     NDIWNSADGVRWTRVAEHAAWSPRNVGVLEFKNRMWIFGGGVIDGHKEINPDSHREIW
                     SSADGINWTPEKMKTERPLGGTPVVFDDKLWFVGANRNDGSFDNAVLVSGDGVTWQAQ
                     SAPWSPRGAVAVWIFDNKLFMTGGKYSYMKNGTPVFVYSNDVWAMSKKTE"
     gene            1248..1832
                     /locus_tag="VGB68_20320"
     CDS             1248..1832
                     /locus_tag="VGB68_20320"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0051655.1"
                     /translation="MKIEMERFALMTENRTEQELLAACRSGDRRAFESLYVQNQKRVF
                     SVALNFFGGDEELAKDITQQVFLKIFRRVADFRGESEFTTWLYRITVNICLDEARKMR
                     PLLSLTDFLGAFGVKRAQDEKVYQREICDEVQKAVAKLKPKFRLPILLKYVEGLSYEE
                     IARVLDCSTGTVASRLNRGHKMLAGKLGHLKNEF"
     gene            1913..2971
                     /locus_tag="VGB68_20325"
     CDS             1913..2971
                     /locus_tag="VGB68_20325"
                     /inference="COORDINATES: protein motif:HMM:NF024807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEY0051656.1"
                     /translation="MLITPAIGCEANDTLSPTAQMTVARAAHTATLLSGGKVLIAGGM
                     QRNDVHTSSAEVYDPATGRFAAAANMNEKRSGHTATRLSDGKILIAGGSNTQWLSSAE
                     VYDPATGAFTPTGNMSVRRGGATATLLADGKVLIVGGFDGSLHASAEIYDPATGKFTV
                     TGNMSAGRSAHTANLLSNGKILIAGGGANRNVLASAELYSPASGTFTPTGNMSVVRHK
                     HAAMRLLNGDVLIVGGSDNLDWRGRYISAEIYKPAAGIFTAVGNMNTARFKLSSAITL
                     LNSGKVFVAGGGAGAETYNPATGTFNAVKGKMDAARFYSTATLLPDGGVLVVGGYNGN
                     IEPSSGAWLYRKQDTKTS"
     gene            3018..4382
                     /locus_tag="VGB68_20330"
     CDS             3018..4382
                     /locus_tag="VGB68_20330"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY0051657.1"
                     /translation="MQEHIQEKISAYLNHELPKEERQAIAEHLLQCEKCRDEHDRIKL
                     GAALASHLKRSDAPENLWNEIENALDAKRQPHVSPVPAFSFFSSRGLPAAAAALLIGL
                     GLVAALYFGLSKNESPELVKNEPAFNQIADTETPPVVFAPGENLSNQNIGAQIPPNPN
                     SNIISPDADWSAQTQPGNTSSNAQAVPKAVSNQTLINPGETLAAANNLPAWNVETLAG
                     TPTAGNEAIGEKGKLAVGQFLETDANSRARVEVSNIGQVEIAPNSRVQLVKTHATEHR
                     LSLRRGLMKAKILAPPRLFIVDTPSAVAVDLGCEYTLEVDAEGNSKLHVTAGFVALER
                     GGRESIVPAGAICFTRRGKGLGTPFSDDASLEFQKAIRRFDFENGGSASLQTIIKESG
                     LYDSLTLWHLLARVPKNEREKVFDALANYVKPPSGVTREGVLRLDKKMLDGWWKEIEN
                     VWFE"
     gene            4496..5734
                     /locus_tag="VGB68_20335"
     CDS             4496..5734
                     /locus_tag="VGB68_20335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051658.1"
                     /translation="MKRSLFVLTVASYLALSLTLLDFGAPKKAEATPSPNDLVVHEWG
                     TFTSIAGKNGAALDWRPLNGASDLPKFVYTGASENGYRETYGSKFEASRKGYVVAKVR
                     METPVIYFYTEREMEVSVNVDFPRGQITEWYPQASVVNGYFAKERTARNPAGGINWGT
                     IKLLPGEKPDYLREVSDSHYYPARETDAVPVQVCNADKTKIEREKFLFYRGVGDFGLP
                     LTVRLSHGKITLVNNGAGEMKNLIVFENRGGKIGFRFVESLGRQTIVERPETSQTLDK
                     VLIELEKILVAEGLYAKEAKAMIKTWRESWFEEGLRVFYILPTKATGEILPLRVEPKP
                     KEIVRVLVGRAEVITPEMEQDVRKQVGLLKSESAEARKEAQKNLQKHGRFYEPILKSL
                     LESEKDAEIRAEIQKLISPG"
     gene            complement(5852..7078)
                     /locus_tag="VGB68_20340"
     CDS             complement(5852..7078)
                     /locus_tag="VGB68_20340"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HEY0051659.1"
                     /translation="MSIIKPFRALRPVREKAEQVSCVPYDVINPAELHALIENNPLSF
                     LRVTRAEAELDAAAETEQSLALARRNLQKLIDEKVFVQEDEPALYVYRLSNGAQTQTG
                     VVACCSLDEYEKGFIKKHEKTREDKVQDRTEHILALRAQTGLIFFTFRGIQAISDLIA
                     ETARGEPLYDFGCPQQISNTVWKVSAAATSDFISAFSEVPALYVADGHHRLEAAARAR
                     RILQAENPNHTGAEDYNFVMAGIFPAEDLQILPYNRVVRDLNGLDEKEFLERLSENFI
                     VGDAAKPTPESRNEFCMYLDGRWRKLRFAVDFVRAPDPLDALDVSILQNYILKTVLGI
                     EDIRTDKRVDFVGGIRGTAELERLVDSGEFKIAFSLFPTTVEDLFQVSDRDEIMPPKS
                     TWFEPKLRDGLLIHLI"
     gene            complement(7183..7695)
                     /locus_tag="VGB68_20345"
     CDS             complement(7183..7695)
                     /locus_tag="VGB68_20345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051660.1"
                     /translation="MKIYKISLVVFIACALVGCSQLQNLVQPKSPTQTMKNFVEATQK
                     KDVEGVKKSLSSGSLKMMEGLAKIQGKTLDETIREGDAGENEYKQVPEMRNEKIEGDT
                     GTLEVKNEKTGDWETLYFVRETEEWKIALDKSLEEMFKKTLGDWKMPDFGNTNSTGSD
                     DAKPSNDKKP"
     gene            complement(7833..8384)
                     /locus_tag="VGB68_20350"
     CDS             complement(7833..8384)
                     /locus_tag="VGB68_20350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051661.1"
                     /translation="MRFYRTFLILISALFFACTASAPKPETPLDTLKAYMQAIKKKDP
                     TEMKLLLSKGSLKMADDEAKAQNTTVDEIIKRETLFTETQKTVEFKNEKIDGERATIE
                     VKNSFGSWDIVPFNKEDGEWKIAKERYVEEQMKKTDEEMKRMDEQFRQQSLPDATAPT
                     FPTPTAMPPTSGVPDTTTPVPTP"
     gene            8602..8889
                     /locus_tag="VGB68_20355"
     CDS             8602..8889
                     /locus_tag="VGB68_20355"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEY0051662.1"
                     /translation="MERRKLSAEEIEANLSGLDDWTAANEKLSKKFEFANFAESLDFV
                     NKVGAIAERRDHHPDILFGWGYAEFFITTHDRGGITDFDFALALEIEKIKS"
     gene            complement(8912..9643)
                     /locus_tag="VGB68_20360"
     CDS             complement(8912..9643)
                     /locus_tag="VGB68_20360"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY0051663.1"
                     /translation="MKNDFSKLFSGFLASSLSGGKQGEIVNRNIPVNDESYLYQAYLP
                     PQAATGEKLPLIVFLHGIRERGSGGYISGMSAQIARQYLRPIPAIVLLPQCRPNQFWA
                     DAPMDEMVMRQIEDARREFSIDERRKYLLGISMGGYGVWHFAAAYPNEFAALVSICGG
                     SPLTEQNRFSAIAEKIGKIPSWLFHGAEDRIVQVSESRNLAAAIRENGGEVKYSEYAG
                     VGHNVWLNALGETRLLPWLLSQEKD"
     gene            complement(9769..>10562)
                     /locus_tag="VGB68_20365"
     CDS             complement(9769..>10562)
                     /locus_tag="VGB68_20365"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF015904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEY0051664.1"
                     /translation="KLSDTPAVFWLDKDRAHDAQIIKKVEKYLQNYDLTGLDIRIMDV
                     KDAMLETLKRAREGKDTISVSGNVLRDYLTDMFPILELGTSAKMLSIVPLMNGGGLFE
                     TGAGGSAPKHIEQFLHEGYLRWDSLGEFLALQASFEHLAQTQGNKKAQILADALDEAN
                     AKFLATDKSPARKVGQIDNRGSHFYLAMYWAESLANQTSDSEIAAKFAPVAKALQENE
                     AKINEELIGAQGNSQDIGGYYHPSSEKTYAAMRPSAALNAIVDGI"
BASE COUNT         2562 a         2655 c         2769 g         2576 t
ORIGIN      
        1 ttcagctcaa tcaagcccgc aatttacccg gtcagaaaat tcgccgcgca cgttcatatg
       61 acgattaccg gaaaaaattt ccgggaagac tggattgatt cgattttgtt tgaagacgac
      121 cgcctgattt ccgctcagga acgaaatcag gcgggaaaac gcggcggttt taatccgatt
      181 ctgaaacttg aaaaaggctc ggacggaatt tttcgcgccg cgcgcgatat tcagctttgg
      241 aaagcttgat ttggagaaaa tatgcgcaaa ctcattagtt ttttatttat attcgtttgt
      301 ttctgcgcgc cggcttcggc ttgtcattca aataaaaaca gctacgtctg gaatgaaatg
      361 accggcagcg ccgcttatcc gcagagttac ggctttccgg tttttgcggc gaatgaaaag
      421 atgtttgctt ttcattacga agcggtttgg aattccgccg acggcgcgaa ctggacgaaa
      481 acggatttgc cgtccgtcaa acgagatgct tacgaaacga gatacgttca atttaataac
      541 gccgtctacg cgctcggaca aaaccggggc aattatgccg tcggcatccg gttcggctcg
      601 acgattcggc gcacgaccga ttttaagacc tgggaaacgc tcgccgaaaa gtcttcgctt
      661 cccgaccgcg tttttcacgg cacgattgtt ttcggcggaa aaatctggct gatgggcggc
      721 tacgacggaa aaaattatta caacgacatt tggaactcgg cggacggcgt gcgctggacg
      781 cgggtcgcgg aacacgccgc gtggagcccg cgcaatgtcg gagttttgga atttaaaaat
      841 cgaatgtgga ttttcggcgg cggcgtcatc gacgggcaca aagaaattaa tccggattca
      901 cacagggaaa tctggtcgtc cgcggacggc atcaattgga cgccggaaaa aatgaaaacc
      961 gaacgcccct taggcggaac gcccgtggtt tttgacgaca agctgtggtt tgtcggcgca
     1021 aatcgcaacg acggcagttt tgacaatgcg gttctggttt ccggcgacgg cgtgacgtgg
     1081 caggcgcaat ctgctccctg gtcgccgcgc ggcgcggtcg ccgtctggat tttcgacaac
     1141 aagcttttta tgaccggcgg caaatattcg tatatgaaaa acggcacgcc ggtattcgtt
     1201 tacagcaacg atgtctgggc gatgagcaaa aaaacggagt agaatctttg aaaatcgaaa
     1261 tggagcgatt cgccttgatg accgaaaacc gaaccgaaca ggaattgctc gcggcttgcc
     1321 ggagcggcga ccgccgggct ttcgaatcgc tctatgtcca aaatcaaaaa cgcgtttttt
     1381 cggtcgctct gaactttttc ggcggcgacg aggaattggc gaaagacatc acgcagcagg
     1441 tttttttgaa aattttccgg cgcgtcgccg actttcgcgg cgaatcggaa tttacgacgt
     1501 ggctttatcg catcacggtg aacatttgtc tggacgaagc gcgaaagatg cgcccgctgc
     1561 tttcgctgac ggattttctc ggagcgttcg gcgtcaaacg cgcgcaggac gaaaaagttt
     1621 accaaaggga aatctgcgac gaagtgcaaa aggcggtcgc gaaattgaaa ccgaaatttc
     1681 gcctgccgat tctgctgaaa tacgtcgaag gtctttcgta cgaggaaatc gccagggttc
     1741 tggattgctc gacgggcacg gtcgcctcgc gcctgaatcg cgggcacaaa atgctggctg
     1801 gcaaattggg acatttgaaa aatgaatttt gacaaaacgc gccgcaaaag cacgatgaaa
     1861 tacaattatg aaacacttaa ataatacttt acgcggtctt tttttattac tgatgcttat
     1921 tacgcccgca atcgggtgtg aagctaacga cactctttcg ccgaccgcac aaatgactgt
     1981 cgcccgcgcc gctcatacgg caacattact ttccggcggc aaagttctaa tcgccggtgg
     2041 aatgcagcgc aacgatgttc acacaagcag cgccgaagtt tacgacccgg cgaccggcag
     2101 atttgccgcc gccgccaata tgaacgaaaa acgctcgggt catacggcaa cccgtctgtc
     2161 cgacggaaag attcttatcg cgggcggctc aaacacgcag tggctttcga gcgccgaagt
     2221 ttacgacccg gcaaccggcg cgtttacgcc gaccggcaat atgtctgtca gacgcggcgg
     2281 cgcgacggca actttactgg cggacggtaa ggtgttaatt gtcggcggct ttgacggcag
     2341 tttacacgcg agcgcggaaa tttatgaccc cgcgaccggc aaatttacgg tgacgggcaa
     2401 tatgagtgcg ggaagaagcg cgcacacggc gaatctttta tcgaacggga aaatattgat
     2461 tgccggcgga ggcgcgaacc ggaatgtcct cgccagcgcg gaactctaca gtcccgcgtc
     2521 gggaactttt actccgaccg gtaatatgag cgttgtccgc cataaacacg cggcaatgcg
     2581 gcttctgaac ggcgacgttc tgattgtcgg cggttcggat aatcttgatt ggcgcgggcg
     2641 ctatatcagc gcggagattt acaaaccggc tgccggaatt tttacggctg tcggcaatat
     2701 gaatacggcg cgtttcaagc tttcgtcggc gataacttta ttaaatagcg gtaaggtttt
     2761 tgttgccggc ggcggcgcag gcgcggagac atacaatccg gcgaccggca ctttcaatgc
     2821 ggtcaaaggc aagatggatg cggcgcgatt ttattcgacg gcaacattat tgccggacgg
     2881 cggagttctc gtcgtcggcg gctataacgg caatatcgaa ccaagctccg gcgcgtggct
     2941 atacagaaag caagatacga agacaagcta atctcatagt cgatttaatt ttttcgcgtt
     3001 ttggtgaata aataaaaatg caagaacaca tacaggaaaa aatttccgca tacctcaatc
     3061 acgagctgcc gaaggaagaa cggcaggcga ttgccgaaca tcttttgcag tgtgaaaaat
     3121 gccgtgacga gcacgaccgg ataaaactcg gcgcggcgct ggcaagtcat ttgaaacgct
     3181 cggacgcgcc cgaaaatctg tggaatgaaa tcgaaaacgc tctggacgca aagcgtcagc
     3241 cgcacgtttc cccggttccg gctttttcgt ttttcagctc gcgcggtttg ccggcggctg
     3301 cggcggcttt gttgattggt ttgggtttgg tcgcagccct ttatttcggt ttgtccaaaa
     3361 acgaatcgcc ggaattggtg aaaaatgagc cggcgtttaa tcaaatcgct gatacggaaa
     3421 cgccgccggt cgttttcgcg ccgggcgaaa atttatcaaa tcaaaacata ggcgcgcaga
     3481 ttccgccaaa tccgaattca aatattattt cgccggacgc agattggagc gcgcagactc
     3541 agcccggaaa tacaagctca aacgctcaag ctgtgccgaa agccgtttcg aatcagacgc
     3601 ttatcaatcc gggagaaacg cttgccgccg caaataatct gcccgcctgg aacgtcgaga
     3661 cgcttgccgg aacgccgacc gccggaaatg aagcgatcgg cgaaaaagga aagctggcgg
     3721 tcgggcagtt tctggaaacc gacgcgaatt cgcgggcgcg cgtcgaggtt tcaaatatcg
     3781 ggcaggtcga aatcgcgccg aattcgcgcg tgcagctcgt caaaacccat gcgaccgagc
     3841 atcgcctgtc gctccggcgc ggattgatga aggcgaaaat tctcgcgccg ccgcgccttt
     3901 ttatcgtcga tacgccgtcg gcggtggcgg tcgatttggg ctgcgaatac acgctcgagg
     3961 tcgacgcgga aggcaacagc aaactgcacg tgacagccgg ttttgtcgcg ctggagcgcg
     4021 gcgggcgcga atcaatcgtt ccggcgggcg cgatttgttt tacgcggcgc ggcaaagggc
     4081 tgggaacgcc tttttccgac gacgcgtcgc tcgaatttca aaaggcgatt cggcgattcg
     4141 attttgaaaa cggcggcagc gcgtcgctgc aaacgattat taaagaatcc ggcttgtatg
     4201 attcgctgac gctctggcat ctgctcgcgc gcgtgccgaa aaacgagcgc gaaaaggttt
     4261 tcgacgcgct cgccaattac gtcaagccgc cgtcaggcgt tacgcgcgaa ggcgttttgc
     4321 gtttggataa aaaaatgctc gacggctggt ggaaggaaat tgaaaacgtc tggtttgagt
     4381 gattgcccgt cgtctttagt cagttgcccg ctgtaaattt aatttaagaa tttaaattca
     4441 gtttcgagat taaattcaac cggcgactgg ctaaggacaa gcaaaggaga aaaatatgaa
     4501 acgcagtcta tttgttttaa ccgtcgccag ctatctggct ttaagtttaa ctctcctcga
     4561 tttcggcgcg ccgaagaaag ctgaagcgac gccttcgccg aatgatctgg tcgtgcacga
     4621 atggggaaca ttcacttcaa tcgccggaaa aaacggcgcg gcgcttgatt ggcgaccttt
     4681 aaacggcgcg tcggacttgc cgaaattcgt ttataccggc gcgagcgaaa acggctaccg
     4741 cgaaacttac ggctcaaaat tcgaagccag ccggaaaggt tacgtcgtcg ccaaagtgcg
     4801 gatggaaacg ccggtgattt atttttatac cgaaagggaa atggaagttt ccgtcaacgt
     4861 cgattttccg cgcggtcaaa tcaccgaatg gtatccgcag gcgagcgtcg tcaacggtta
     4921 ttttgcgaag gaacggactg cccgaaatcc ggcgggcggc ataaactggg gaacaatcaa
     4981 gcttctgccc ggcgaaaaac cggattacct gcgcgaagtc tccgacagcc attattaccc
     5041 ggcgcgcgaa accgacgccg ttccggttca ggtctgcaac gccgataaaa ccaaaatcga
     5101 aagggaaaag tttttgtttt atcgcggcgt cggcgatttc ggtctgcctt tgaccgtgag
     5161 attaagccat ggaaaaatca ctctggtcaa taacggcgcg ggcgaaatga aaaatctcat
     5221 cgtctttgaa aatcgcggcg gaaaaatcgg tttccggttt gtcgaatcgc tcggcaggca
     5281 aacaatcgtc gagcgtccgg aaacttcgca aacgctcgat aaagtcttaa tcgagctgga
     5341 aaaaattctg gtcgccgaag ggctctacgc gaaagaagcc aaagcgatga taaaaacctg
     5401 gcgggagagc tggtttgaag aaggtttgcg ggttttctac attttgccga ccaaagcgac
     5461 cggcgaaatt ctgcctttgc gcgtcgagcc gaagccgaaa gaaatcgtca gggttttagt
     5521 cgggcgcgcc gaggtcatca cgccggaaat ggagcaggac gttcggaaac aagtcgggct
     5581 tttgaaatcg gagtcggctg aagcgcggaa agaagcgcaa aagaatttgc agaaacacgg
     5641 cagattttac gagccgattc tgaaaagcct tttagagagc gagaaagacg ccgaaattcg
     5701 ggcggagatt cagaaactta tttcacccgg ttgaaattta acaggatgga caggatacac
     5761 aggatgaaaa tttttttatt caaaagcgct gaacaaaatt cgaatgacga tttgaaaaac
     5821 aatttatcct gtccatcctg ttaaatttcg cctaaatcaa atgaatcaaa agcccgtctc
     5881 ttaatttcgg ctcaaaccag gtcgatttcg gcggcataat ttcatctctg tccgaaacct
     5941 gaaacaaatc ctcgaccgtc gtcggaaaca gcgaaaaagc gattttaaat tcgcccgaat
     6001 cgaccagccg ctccagctcc gccgtgccgc gaatgccgcc gacgaaatcg acgcgtttgt
     6061 cggtgcgaat gtcttcgatt ccgagaaccg ttttcaaaat ataattttgc aaaatgctga
     6121 cgtcgagcgc atcgagcggg tcgggcgcgc gaacgaaatc gacggcgaag cggagcttgc
     6181 gccaccgccc gtcgagatac atacaaaatt cgttccggct ttccggcgtc ggtttcgcgg
     6241 cgtcgccgac gatgaaattt tcgctcaggc gttccagaaa ttctttttcg tcgagtccgt
     6301 tcaaatcgcg cacgacgcgg ttatacggca aaatctgcaa atcttcagcc ggaaaaattc
     6361 ccgccattac gaagttgtaa tcttccgcgc cggtgtgatt cgggttttcg gcttgcagga
     6421 tgcggcgggc gcgggcggcg gcttcgaggc gatgatgacc gtcggcgacg tacagcgccg
     6481 gcacttccga gaaggcggag atgaaatcgg atgtcgccgc cgcggaaact ttccagacgg
     6541 tgttggagat ttgctgcggg cagccgaaat cataaagcgg ctcgcccctg gcggtttccg
     6601 caatcaaatc ggaaatcgcc tgtattccgc gaaaagtaaa aaatatcaag cccgtctgcg
     6661 cgcgcagggc gagaatgtgc tcggttctgt cctggacttt gtcttcgcgc gttttttcgt
     6721 gttttttgat aaaacctttt tcgtattcgt cgagcgagca gcaggcgacg acgcccgtct
     6781 gcgtctgcgc gccgttcgac aggcgataaa catacagcgc cggctcgtct tcctgtacaa
     6841 aaactttttc gtcaatcagt ttttgcagat tccggcgcgc cagcgccagc gattgctcgg
     6901 tttccgccgc cgcgtccagc tcggcttcgg cgcgcgtcac gcgcagaaag ctgagcggat
     6961 tgttttcaat aagcgcgtgc agctccgccg gattgataac gtcgtaaggc acacaggaaa
     7021 cctgctctgc tttttccctg accgggcgca aagcacgaaa aggtttgatt attgacacgg
     7081 aaaattctcc taataaagtg aaaagtgaaa aagtgaaaaa gcagttttat agagaaataa
     7141 cataatcttt ttcagttttc tgctttttca cgcttttaaa gcttacggtt ttttgtcgtt
     7201 cgatggcttg gcgtcgtcgc tgccggtcga attggtattg ccgaaatcgg gcatcttcca
     7261 gtcgccgagc gttttcttga acatctcttc gagcgattta tccagcgcga ttttccattc
     7321 ttccgtttcc ctgacgaaat aaagcgtctc ccaatcgccg gttttttcgt tttttacttc
     7381 cagcgttccc gtgtcgccct cgattttttc gttgcgcatt tccggcactt gcttatattc
     7441 gttctcgccc gcgtcgcctt ctcttatcgt ttcgtcgagc gttttgccct gtatcttggc
     7501 gagcccttcc atcattttca gcgagccgct ggagagcgat tttttcacgc cttcgacatc
     7561 ttttttctgt gtcgcttcga caaaattttt cattgtctgc gtcggcgatt tcggctgcac
     7621 taaattttgt aactggctgc acccgaccaa agcgcacgcg ataaacacga cgagcgaaat
     7681 tttatagatt ttcatatttt cctttcaggt aatttaaaat gtgcaagtat aactcaaaaa
     7741 ccgtttattt ttcagaaacg aaagacacat tttctcggaa gaaaacggct cgcgccggag
     7801 taaaataaca aaaccgcctc gccttcttca agttacggcg tcggcacagg cgttgtcgtg
     7861 tcgggcacgc ccgatgtcgg cggcatcgcc gtcggcgtcg gaaaagtcgg cgcggtcgcg
     7921 tccggtaagc tctgctgccg gaattgctcg tccatacgtt tcatttcctc gtccgttttt
     7981 ttcatctgtt cttcgacata gcgctccttg gcaattttcc attcgccgtc ttccttgtta
     8041 aaaggcacga tgtcccacga gccgaacgag tttttgactt caatcgtcgc gcgctcgccg
     8101 tcgatttttt cgtttttaaa ttcgacggtt ttctgcgttt cggtaaaaag cgtttcgcgt
     8161 ttgatgattt cgtcaaccgt cgtgttttgc gctttggctt cgtcgtccgc cattttcagc
     8221 gagcctttgg aaagcaaaag cttcatctcg gtcgggtctt ttttcttgat ggcttgcata
     8281 taagccttca gcgtgtcgag cggcgtttcc ggcttcggcg cgctcgcggt gcaggcgaaa
     8341 aacaaggcgg aaattaaaat taaaaatgtt cgataaaatc gcattgcgtt taataacctt
     8401 taaaaaatag ttatactcaa aatgaaaagt ataacttaaa atcggctgcg gcggcgactt
     8461 tgcaaaagcg aagacccgcg cggcgattga ttgggattgt ttgagaaatt ttcggataaa
     8521 atcataatta attccggatt atcccgaata atccgcgccg catcgtttta tgattacaat
     8581 ttttttcgag gagcaaaacg aatggagaga cgaaaattat cggcggaaga aatcgaagcg
     8641 aatttgtccg gtttagacga ttggacggcg gcaaacgaaa agctctcgaa aaaattcgag
     8701 tttgcaaatt tcgccgagag cctcgatttc gtcaacaaag tcggcgcaat cgccgagcgc
     8761 cgcgaccatc atcccgacat tttattcggc tggggctacg ccgaattttt tatcacgacg
     8821 cacgaccgcg gcggcattac ggatttcgat ttcgcgctcg ctctggaaat cgaaaaaatc
     8881 aaatcctgaa atttcttcga gctcgaagcg gtcaatcttt ctcctgcgag agcagccacg
     8941 gcaaaagccg tgtttcgccg agcgcgttga gccagacgtt atgcccgacg cccgcgtatt
     9001 cggaatattt cacctcgccg ccgttttctc tgatcgccgc cgccagattt ctcgattcgc
     9061 tgacctgcac gatacgatct tcggctccgt gaaagagcca cgaaggaatt ttgccgatct
     9121 tttcggcaat cgccgagaaa cgattttgct ctgtcagagg acttccgccg cagattgaaa
     9181 cgagcgccgc aaattcgttc ggatacgcgg cggcaaaatg ccagacgccg taaccgccca
     9241 tcgaaatgcc caacaaatat tttcggcgct cgtcgatcga aaactcccgg cgcgcgtctt
     9301 cgatttgccg catcaccatt tcgtccatcg gcgcgtcagc ccaaaattga ttcggtcggc
     9361 actgcggaag aagcacgatt gccggtatcg gtcgcagata ttgcctggct atctgcgcgg
     9421 acattcccga aatataaccg ccgctgccgc gctcgcgaat tccgtggaga aaaacgatga
     9481 gcggcaattt ttcgccggtc gcggcttgag gcggtaaata cgcttgataa aggtagcttt
     9541 catcgtttac cgggatgttt cgattaacga tttcgccttg tttcccgcct gaaagcgacg
     9601 aggcgaggaa gccggaaaag agttttgaga agtcattttt catatctttt taactttacc
     9661 gcgccgcaat gacaggtttc aattcgcccg caattcaagc aacaggcgta taaaataaaa
     9721 aaactcccga aatttcggga gttttttatg aattttgaat ttaagctctt aaattccgtc
     9781 cacaatggcg ttcagcgcgg cggaaggtct catcgcggcg taagtttttt cagaactcgg
     9841 atgataataa ccgccgatat cctgtgaatt tccttgcgct ccgatcaatt cttcgttaat
     9901 ttttgcttcg ttttcctgca atgctttggc aaccggcgcg aattttgcag cgatttcaga
     9961 atccgatgtt tgatttgcca agctttccgc ccagtacatt gccaggtaga aatgagagcc
    10021 gcggttatcg atttgcccga cttttctcgc cggagatttg tcggtcgcta aaaacttcgc
    10081 attggcttcg tccaaagcat ctgccagaat ctgcgctttt ttattgcctt gagtttgagc
    10141 caaatgctcg aaacttgcct gcaaagctaa aaattctccg agcgagtccc agcgcagata
    10201 tccttcgtgc aaaaattgtt cgatgtgttt cggcgcgctt cctccggctc cggtctcgaa
    10261 caaaccgccg ccgttcataa gcggaactat ggaaagcatt ttcgcggaag tgccaagctc
    10321 cagaatcggg aacatatccg tcagataatc ccgcaaaaca tttccggaaa ccgaaatcgt
    10381 gtctttgcct tctctggcgc gtttcagagt ttcgagcatc gcgtctttga catccataat
    10441 tctgatgtcg agaccggtta aatcataatt ttgcagatat ttctctactt tttttattat
    10501 ctgcgcgtcg tgcgccctgt ctttgtcgag ccagaaaacg gcgggcgtgt cggaaagttt
    10561 gg
//
LOCUS       DASWNE010001067         1981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_347591, whole genome shotgun sequence.
ACCESSION   DASWNE010001067 DASWNE010000000
VERSION     DASWNE010001067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347591"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VGB68_20370"
     CDS             <1..383
                     /locus_tag="VGB68_20370"
                     /inference="COORDINATES: protein motif:HMM:NF014864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051665.1"
                     /translation="IDARRIGRQEELAELLEKKGFSVTQSSVSRDLLDLGIIKVHGHY
                     ALPKKMRNGVAFGLLNLDTAGENLIVAKCESGLASAVAVRVDDANIAEIVGTIAGDDT
                     IFIAVKDKSEQKTVIKKIWEIFEE"
     gene            437..1636
                     /locus_tag="VGB68_20375"
     CDS             437..1636
                     /locus_tag="VGB68_20375"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF001770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEY0051666.1"
                     /translation="MEKKINKIVLAYSGGLDTSAMLLWLKEAYGCEVVCYTADVGQGE
                     ELDGLQEKALKTGASKLYIEDLREEFVREYVWLAVKANALYEGVYLLGTSLARPVIAK
                     KQIEIARAEGADAVAHGATGKGNDQVRFELTYYALEPDIKVVAPWRQWDFKGRADLIA
                     YCAKHDIDITATAEKPYSTDRNLMHISYEGGILEDPWTAPPEDIFLLTKAPEKASDKA
                     EEIVLTFEKGEPVAIDGEKYGAVDMLSKLNFIGGEHGIGRVDLVENRFVGMKSRGIYE
                     TPGVTILQTAHRALESITLDREVVRLRDSLGVKIAEAIYYGFWFSPEFELMKNLVNQT
                     QTNVSGEVRLKLYRGNVTVSGRRSPYSLYKERIVTFEDDAGAYDQKDAEGFIKLQALR
                     LRLRKFD"
     gene            1767..>1981
                     /locus_tag="VGB68_20380"
     CDS             1767..>1981
                     /locus_tag="VGB68_20380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051667.1"
                     /translation="MILEADKIGSATSPLNLTKTVAAISANDAPKAGDVVVVRALSES
                     VTYGNLELPSGRLAKINRNDRVLGVLG"
BASE COUNT          525 a          457 c          568 g          431 t
ORIGIN      
        1 taatcgatgc caggcggatt ggcagacagg aagagcttgc cgagcttttg gaaaagaaag
       61 ggttttcggt aacgcaatcg agcgtctcgc gcgatttgct cgatttgggc atcatcaaag
      121 ttcacggtca ttacgctttg ccgaaaaaga tgcgtaacgg cgtcgctttc gggctgctga
      181 atctggatac ggcgggcgaa aacctgattg tggcaaagtg tgaatcggga ttggcttcgg
      241 cggtcgccgt gcgcgtcgac gatgcgaata tcgctgaaat cgtcggcacg attgccggcg
      301 acgacacgat ttttattgcc gtcaaagata aaagcgagca aaaaaccgtt attaaaaaaa
      361 tttgggaaat atttgaagaa taagtcttcg gaaaagcaaa agccgaagac cgaaaaccaa
      421 aaaccggaaa tcgaaaatgg aaaagaaaat caacaaaata gtcctggcat attcgggcgg
      481 attggacacg tcggcgatgc ttttgtggct gaaggaagca tacggctgcg aagtcgtctg
      541 ctatacggcg gatgtcgggc agggcgaaga gctcgacgga ttgcaggaaa aagctttaaa
      601 aaccggcgct tcaaaactgt atatcgaaga tttgcgcgag gagtttgtca gggaatacgt
      661 ctggcttgcc gtcaaagcca acgcgcttta cgaaggcgta tatcttttgg gaacttcgct
      721 ggcgcgtccg gttatcgcca aaaaacaaat cgaaatcgct cgcgccgaag gcgctgacgc
      781 ggtcgctcac ggcgccaccg gaaagggcaa cgaccaggtg cggttcgagc tgacttatta
      841 cgcgcttgag ccggacatta aagtcgtcgc gccgtggcgt caatgggatt ttaaaggtcg
      901 cgccgatttg attgcgtatt gcgcgaaaca cgacatcgac atcacggcga cagccgaaaa
      961 accgtattcg acggacagaa atttgatgca catttcctac gagggcggaa ttctggaaga
     1021 cccctggacg gcgccgccgg aagatatttt tctgctgacg aaagcgccgg aaaaggcttc
     1081 ggacaaagcc gaagaaatcg ttctgacgtt tgaaaaaggc gagccggtcg ccatcgacgg
     1141 cgaaaaatac ggcgcggtcg atatgctttc caaattaaat tttatcggcg gcgagcacgg
     1201 catcgggcgc gtcgatctgg tcgaaaatcg tttcgtcggg atgaaatcgc gcggcattta
     1261 cgaaacgccc ggcgtgacga ttttgcagac ggcgcatcgc gcgctggagt caatcacgct
     1321 cgaccgcgaa gtcgtgcgcc tgcgcgacag cctgggcgtc aaaatcgccg aggcgattta
     1381 ttacggcttc tggttttcgc ccgaatttga attgatgaaa aatctcgtca accagacgca
     1441 gacgaatgtt tcgggcgaag tgcgtttgaa gctgtatcgc ggaaacgtga ccgtcagcgg
     1501 caggcgctcg ccctactcgc tttacaaaga gcgaatcgtc actttcgagg acgacgccgg
     1561 cgcgtatgac cagaaggacg cggaaggatt tattaaatta caggcgctgc gcttgagatt
     1621 acgaaaattc gattagctga aagtgtttga taacgggatg gacaggatgt ttgaaagaaa
     1681 tttaacgaaa atatttggaa tttatccagc ctatcctact tctatcccgt tgaaatttcc
     1741 ggttggtttt tgaggtaaaa gaaatgatga ttttagaagc agataaaatc ggttcggcga
     1801 cttcgccgct gaatttaacg aaaacggttg ccgcgatttc cgccaacgac gctccgaaag
     1861 cgggcgatgt cgtcgtcgtg cgcgctttga gcgagtccgt cacatacggc aatctggagt
     1921 tgccgagcgg tcgactggcg aaaatcaacc gcaacgaccg cgttctcggc gttctcggca
     1981 a
//
LOCUS       DASWNE010001068         2690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_347775, whole genome shotgun sequence.
ACCESSION   DASWNE010001068 DASWNE010000000
VERSION     DASWNE010001068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347775"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VGB68_20385"
     CDS             <1..417
                     /locus_tag="VGB68_20385"
                     /inference="COORDINATES: protein motif:HMM:NF014895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEY0051668.1"
                     /translation="NLGTNDLVQYLLAVDRDNEAVADWFRTLHPAVLRAVKTVVRAAE
                     NKNIPLIVCGEMAGSTVYVPILIGLGATELSMNVNSIPRVRQIICNIAFEEAHAIVRE
                     LESCRTAAEIEKAVENLFSTKWKHLFPEEIFSVGKK"
     gene            588..1691
                     /locus_tag="VGB68_20390"
     CDS             588..1691
                     /locus_tag="VGB68_20390"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEY0051669.1"
                     /translation="MKKTFFKIVLFAVGVLAVLPAGDSYAQTTSNPIVKRNSPFSPNP
                     KKKAEIAPKASEEVKNTTESPVKTESAKLMTSESAGALQTENNRTDEPEKQTAEFESR
                     SVAKKTLDIAKRSSSNLTASPTEIYKVGAGDVLDIRLINSPAKDSTLFTVLESGAIDY
                     PLAGEEALQVAGLTPEEIENLLKEKVKLYENPSISVTVRDYSSHTISVFGLVEKPGTK
                     ALRREAIPLYVILAEAIPQQSANKVTILRSVDKQTITVNLEDNETLIYPNDIVRVASG
                     GGKNSYGQTQFYYIGGEIASGGQKDFTDGLTLTQAILACGGLKKSSARKVIIRRRDGQ
                     GKLVPTEFDLKFIKEGKIADPILKAGDTIEIGN"
     gene            1760..2153
                     /gene="rnpB"
                     /locus_tag="VGB68_20395"
     ncRNA           1760..2153
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VGB68_20395"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            2318..2590
                     /locus_tag="VGB68_20400"
     CDS             2318..2590
                     /locus_tag="VGB68_20400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051670.1"
                     /translation="MANRKGVFIGAYVPNELKESLRRRAALEHRTLSQEITRILVEAV
                     HGKGLPAGSVDRRGGTTVPRRRATDPLPRRRAEDLPLIGSEKEDKK"
BASE COUNT          756 a          644 c          700 g          590 t
ORIGIN      
        1 aatctcggaa caaacgattt agtgcagtat ctgttagccg tcgaccgcga caacgaagcc
       61 gtcgccgatt ggttccggac gctgcatccg gcggttctgc gcgcggtcaa aacggtcgtc
      121 cgcgccgctg aaaacaaaaa tattccttta atcgtctgcg gcgagatggc tggctcgacc
      181 gtctacgtcc cgatcttgat cggactcggc gcgaccgagc tgagtatgaa cgtcaactcg
      241 attccgcgcg tcaggcaaat tatctgcaac atcgctttcg aagaagcgca cgccatcgtc
      301 agagagctgg aaagctgccg caccgccgcc gaaatcgaga aagcggtgga aaacctcttc
      361 agcacgaaat ggaaacatct ttttccggag gaaatctttt ccgtcggcaa aaaataaatt
      421 ttttcacaaa atactagaat aaaagggctt ttagtttttt atagaaacgc gggcgaaaat
      481 aatgtgccga agttctaaaa aattacttgc ctgaagccgt ctgtctgatt tatctttgtt
      541 gcatccaaaa tcaaccagac gaatccgggc gttagcggaa agctattatg aaaaagactt
      601 tctttaaaat tgttttgttt gcagtcggcg ttctggcggt tttgccggcg ggcgattctt
      661 acgcgcagac gacgtcgaat ccaatcgtga aacgaaactc gccgttttcg ccgaacccga
      721 agaaaaaagc cgaaatcgcg ccgaaagctt ccgaagaagt taaaaatacg acggaaagcc
      781 cggtcaaaac cgaaagcgcg aaactgatga ccagcgagag cgccggcgct ctgcaaacgg
      841 aaaataaccg gacggacgag ccggaaaaac aaaccgcgga atttgaaagc cgctcggtcg
      901 cgaaaaaaac tctggacatc gccaagcgca gcagcagcaa tttgacagcg tcgccgacgg
      961 aaatttacaa ggtcggcgcg ggcgacgttc tggatattcg cctgattaat tcgcccgcca
     1021 aagattcgac gcttttcacc gttctggaaa gcggcgctat cgattacccg ctcgccggag
     1081 aagaagccct tcaggtcgcc ggattaacgc ccgaggaaat tgaaaattta ttaaaagaaa
     1141 aggtcaagct ttacgaaaat ccgtcaatca gcgtgacggt cagagattat tcgagtcaca
     1201 ccatcagcgt tttcggttta gtcgaaaaac ccggaacgaa agccctgcgc cgcgaggcga
     1261 ttccgcttta cgtaattctg gcggaagcca taccgcaaca gtcggcaaat aaagtgacga
     1321 ttctgcgcag cgtcgataaa cagacgatta ccgtcaatct ggaagacaac gaaacgctca
     1381 tctacccgaa cgatattgtc cgcgtcgcca gcggcggcgg aaaaaattct tacggtcaaa
     1441 cgcagtttta ttacatcggc ggcgagattg cctcgggcgg gcagaaggat tttaccgacg
     1501 gtctgacgct gacgcaggct atcttagcgt gcggcggctt gaaaaaatct tcggcgcgca
     1561 aagtcattat tcgccgccgg gacgggcagg gaaagctggt tccgaccgag ttcgatttga
     1621 aatttatcaa agaagggaaa attgccgacc cgattctgaa agccggagat acaattgaaa
     1681 tcggcaactg aaaatctata taaactcgga ttggttttgt tgaacatttt ttgttaaact
     1741 gaaccagcct tgcaaaattg gagcaagtcg ggcaatcgct gccggaaatt ttttcgggag
     1801 aggaaagtcc gaacaccgaa gggcggcgag ccggataacg tccgggcgtt ttttcgcaag
     1861 agagagcgac gacaagtgca acagaaaaca aaccagccgg agacggtgat gggtgaaacg
     1921 ttgcggtaag agcgcaacag cgcgcgtggt aacaggcgcg gctgggtaaa ctcctcgcgg
     1981 tgcaagacca aataggaaaa cgttatcagg gttgcccgct ctgatgtttg agcaatcaaa
     2041 cggcgttttc gggtaggtcg ctggagctgt ttggcaacaa acagcctaga tgaatgattg
     2101 ccgctttgaa tcttttcaaa gaacaaaatt cggcttacag acttgctcca ggcataggcg
     2161 tcaaaaatgc aaaacgaaaa attcagaatg caaaatgaaa ggataaattt aatttcggaa
     2221 ttctaaatcc tgaattttga atccgaatat ttcgcacttt gcatttttcg ttttgcatta
     2281 ataaacattt cctaaaaatt aaggagagat tgtcataatg gctaaccgta aaggtgtttt
     2341 tatcggcgcg tacgtgccga atgaattaaa agaatctttg cgccgtcgcg cggcgctgga
     2401 gcatcggacg ctgtcgcagg aaatcacccg aattctggtc gaagcggttc acggcaaagg
     2461 cttgcccgcc ggaagcgtcg accgtcgcgg cggaacgacc gttccgcgtc gccgcgccac
     2521 tgacccgctg ccgcgtcgcc gcgccgaaga tttgccgctt atcgggtccg aaaaagaaga
     2581 taaaaaataa atcggtcgga attctgcggg aaagcgtcgt cgccgggttc atcggcgatg
     2641 gcgcttttcg ctttttgaaa acgttttgct aaacttttcc tgaatgaata
//
LOCUS       DASWNE010001069         5168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_632525, whole genome shotgun sequence.
ACCESSION   DASWNE010001069 DASWNE010000000
VERSION     DASWNE010001069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5168
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632525"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..3059
                     /locus_tag="VGB68_20405"
     CDS             51..3059
                     /locus_tag="VGB68_20405"
                     /inference="COORDINATES: protein motif:HMM:NF013003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEY0051671.1"
                     /translation="MPRSSGLPVKKPVSVWNREVKVNFRDLFKALGKAVVSAKVAKFD
                     LAISSLVDSVAAFELKKDAGQMAWLLICRSISRAMFMLIEDNLPLIREATRGFRNLAG
                     ESKPSEENLQAIADKLDYAVDARDLAIDRKFFERPREFPVIEDLKKPLAQWLQGFGLN
                     SSQAHSICGRFPSYFVFALNDEWRERRADYNLVTEEIETPFTKAAEIERAWSHYYASL
                     QKRVDEPLFQEAFSLRQIYIPLRAYFEQRKESDAREKFFENDMYIERSFEKVVIDLEE
                     ELESWVDNSDEREAIRILSGGPGYGKSSVARMFAAKQSEKSDRRVLFIPLHLFEPTED
                     LVDSIKKFVEAEDFFPPVNFLHPVEGEKRLLLIFDGLDELSMQGKVGNEIVRQFVKEV
                     QRKADILNRRECRLKVLICGRDISVQTSIDSLDRKTKVLNLLPYFVNEYERDNYIDKN
                     KFLIEDKRNLWWKKYGKVIGKDYRALPAELNKENLTEITALPLLNYLVALSYTRGKID
                     FARENNLNSIYQDLLESVYQREDVWADKQHTILEGVTERDFFRIFEEIALAAWHSGEG
                     RKVSIKEIEKKCEDSGLKPLLEKFQKKAKDGVASLLIAFYFRKAGVQAENLNETFEFT
                     HKSFTEYLTARRIVRGIQIISDEMKRRQASYEIGWDEISALTHWVKLCGLNAVDEYLL
                     DFIRDEIALMPIEIVMEWQKSLGALFSHVLRYGMPMEKLADDRPNFKEEIRRAQNSEE
                     ALLVVLNACALVTKQISDINFPNEFCLEEWLRWIVTPVSREVLTFMSFEKAPFLYGAN
                     LEGANLEQVNLSKTNLAAAQLSKARLTLAKLERANLSGANLSGAVLWGANLNWAKLAE
                     ANLSGANLEMAILTGALLRDASLKDATLTGANLSEAMLTGCNLSRAVLTEASLSRAVI
                     EKSHLEESVLIRADLSEANLSGANLYGAVLEKTNLKRCNLAGANLERAYLLEANLEGA
                     YLSGAIWIDGRKIIGGKYPDLIFEGDKPEK"
     gene            complement(3371..4642)
                     /locus_tag="VGB68_20410"
     CDS             complement(3371..4642)
                     /locus_tag="VGB68_20410"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY0051672.1"
                     /translation="MKRNFSASFPAEIMRMAFESIYANKFRSTLTILGIVVGVMTAIV
                     VASILTGMRQSIISIVEEYGTNNIYVFHLSTGPGARNRDERNRKPLTPEDAQAIMTQS
                     AAIDDIALIAPSIGSWGDGFDDNLVYEGRNYRWALTDGVTPNYMQVTNLVLRQGRFIT
                     ESDNYQRRNVIVVGVNTVEALFPNEQESVVGKVVRMNGTTWEIIGVIEKRKAGFFGEN
                     EEDRKVFLPFRTAQKVAPQRDAVLHIIQAKPGQINDAVQDVESILRQRRGVKFDEANN
                     FDVKTADKFIAQFDGIIGGVGIAAIAISCLGLLVGGIGVMNIMLVSVTERTKEIGIRK
                     AIGATRGAIVLQFLLEAMTLTFFGGVIGVSLAIVISYLVMLLIPSLPASIPLWAILLG
                     LGVSIGVGLIFGVLPARKASRLDPIECLRYE"
     gene            complement(4662..>5168)
                     /locus_tag="VGB68_20415"
     CDS             complement(4662..>5168)
                     /locus_tag="VGB68_20415"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY0051673.1"
                     /translation="AIEEARLLLRNKRGLLGSEEDTFSLVNVDEFNSQMDQFTNAIAA
                     VVVPITMITLIVGGIVVMNIMLVSVTERTFEVGLRKALGATRKQILLQFLIESSLLCI
                     IGGIIGLLLAMGVTQLITFLLEMTMTITIFYVIMSVGVSSIIGIVAGLYPAWKAARLD
                     PIVALTQS"
BASE COUNT         1379 a         1225 c         1333 g         1231 t
ORIGIN      
        1 cgaagtaata ttcctcacaa taaaattact tcgcgaagga aaataaattt atgccacgct
       61 catccggctt acccgtcaaa aagccagttt ccgtctggaa tcgtgaagtc aaagtaaact
      121 tcagagacct tttcaaagct ctcggcaaag ccgtcgtcag cgccaaggtc gccaaattcg
      181 atttggcaat cagcagttta gtcgattccg tcgccgcctt cgaattaaaa aaagacgccg
      241 gacaaatggc ttggctttta atctgtcgct cgatttccag agcgatgttt atgctgattg
      301 aagacaatct gcccctgatt cgcgaagcga ccagaggctt ccgaaatctt gccggtgaat
      361 cgaaaccgag cgaagaaaat ctccaagcca tcgccgacaa actggattac gccgtcgatg
      421 ccagagattt ggcgattgac cgcaaatttt ttgaacgccc gcgcgagttt ccggttatcg
      481 aagacctcaa aaagccgctc gcccaatggc tccagggctt cggcttaaat tcttctcaag
      541 cccattcaat ctgcgggcgc tttccttcat atttcgtctt cgcgctcaat gacgaatggc
      601 gcgagcgccg cgccgattac aatctcgtca ccgaagaaat cgaaacgcct ttcaccaaag
      661 ccgccgaaat cgagcgagcc tggtcgcatt attacgcgtc gctgcaaaaa cgcgtcgatg
      721 agcctctatt tcaggaagcg ttcagcctgc gtcaaatcta tattccgtta cgagcatatt
      781 tcgagcaaag aaaagaaagc gatgcgcgcg agaaattctt cgaaaacgat atgtatatcg
      841 aaaggagttt tgaaaaagtc gttattgatt tggaagaaga actcgaaagc tgggtcgata
      901 attccgatga aagggaagct atccggattc tcagcggcgg tccgggttac gggaaatctt
      961 cggtagccag gatgtttgcc gcaaaacaat cggagaaaag cgaccgccgc gttttattta
     1021 ttccgctgca tcttttcgag ccgacggaag atttagtcga ttcgattaaa aaatttgtcg
     1081 aagcggaaga ctttttcccg cccgttaatt ttcttcaccc ggtagaaggc gaaaagcgtc
     1141 ttttgctcat ttttgacggt ttggacgagc tttcgatgca gggcaaagtc ggcaacgaaa
     1201 ttgtccggca gtttgtcaag gaagttcagc gcaaagccga cattctcaat cgccgcgaat
     1261 gtcgtttgaa ggttctaatc tgcgggcgtg atatttccgt gcagaccagt atagatagtc
     1321 tggatagaaa aacaaaagtc ttaaacctgc tgccgtattt tgtgaatgaa tatgaaaggg
     1381 ataattatat agataaaaac aaatttttaa ttgaagacaa aagaaatctt tggtggaaaa
     1441 aatacggcaa ggtaatagga aaagactaca gggctctacc cgcagagctt aacaaagaaa
     1501 atctgacgga aattacggct ttgccattgt taaattatct ggttgcgctc agctatacac
     1561 gcggaaaaat tgattttgct cgggaaaaca atcttaattc aatttatcaa gaccttctgg
     1621 aatcggttta tcagcgcgaa gacgtctggg ctgataaaca acatacgatt ttagaaggag
     1681 tgacggagag agactttttt cgcatttttg aagaaattgc tctggcggcg tggcatagcg
     1741 gagaaggtcg caaggtctcg attaaagaaa ttgagaaaaa atgcgaagac agcggattga
     1801 aaccgctgct cgaaaaattt cagaaaaaag ccaaagacgg cgtcgcttca ttattaatcg
     1861 cgttttattt caggaaagcg ggcgttcagg cagagaattt aaacgaaact ttcgaattta
     1921 cacataagag cttcaccgag tatctgacgg cgcgtcgaat cgtgcgcggc atccaaatca
     1981 tcagcgacga aatgaagcgg cggcaagcat cttatgaaat cgggtgggac gaaatatcgg
     2041 ctttaacgca ctgggtgaaa ttgtgcggat taaatgcggt cgacgagtat ctgctggatt
     2101 ttatcaggga cgaaattgca ttgatgccga tagaaatcgt tatggaatgg caaaaatctc
     2161 tgggcgcgtt attcagccac gttttgcggt atggaatgcc gatggagaaa ctagccgacg
     2221 accgaccgaa ttttaaggaa gagattcgtc gggcgcaaaa ttccgaagaa gcgcttttag
     2281 tcgtcctaaa tgcttgcgct ttagtaacaa aacaaatttc tgatattaat tttccgaacg
     2341 aattttgcct tgaagaatgg ctccggtgga ttgttacgcc ggtctcccgc gaagttttga
     2401 catttatgtc ttttgaaaaa gcgccctttc tttacggagc aaatctggaa ggagcaaatc
     2461 tggaacaggt aaatctcagt aagacgaatc tggcagcggc gcagctttcc aaggcaaggc
     2521 ttacattggc gaagcttgaa agagccaatc tttcgggagc caatctttcg ggagccgttc
     2581 tttggggcgc taatcttaat tgggcgaaac ttgcggaagc aaatctgagc ggagcaaatc
     2641 tcgaaatggc aattctcacc ggcgcgcttc tcagagatgc atcgttaaaa gatgcaacgc
     2701 tcacgggcgc caatctttcg gaagcaatgc tcacgggctg taatctgtcg cgagctgtcc
     2761 tgaccgaagc cagtctatcg cgtgccgtta tcgaaaaatc tcatttggag gaaagcgttc
     2821 tgataagagc cgacctttcg gaagcgaatc tttccggggc gaatctttac ggggcggttc
     2881 ttgaaaagac caatcttaaa cgttgtaatt tggcgggcgc aaatctggaa agagcatatc
     2941 ttttggaagc aaatctggaa ggagcttatc tgtcgggcgc gatttggata gatggcagaa
     3001 aaattattgg cggcaagtat cctgatttga tttttgaagg tgataaaccg gagaagtaat
     3061 cgagtaaaaa gaaatattcg gcttgatcgt tgaaaaaaaa ctttaacccg gcgtgaacgg
     3121 gcgcggtcga tatcgtttct ttccgagtga aagccgagcc tccgactcgc aaggttaaaa
     3181 cccgtgtccg ccgtcttaaa accgccttcc gccgcctcaa aactcgcgac cgccaaagta
     3241 aaaccggcga ccgccaggtt aaacctgaca gccgccgcct tgaaactttc tagcttccgc
     3301 caaataaaat ctaacgcccg ccgaaacgcg ggaagcgtac gagattgatt acgaggcgat
     3361 gaaaattcgt ttattcatag cgcagacact caatcgggtc taatcgcgac gcttttcgtg
     3421 ccgggagaac gccgaagatt aagccgacgc cgatggagac gcccaagccg agcaggattg
     3481 cccagagcgg aatcgaagcc ggcagactcg gtatcagaag cattacgaga tagctgatga
     3541 cgatggctaa gctgacgccg atgacgccgc cgaaaaacgt taaggtcatc gcttcgagca
     3601 gaaattgcag gacgattgcg ccgcgcgtcg cgccgatggc tttgcggatg ccgatttctt
     3661 ttgttctttc ggtgacggag acgagcataa tattcatcac gccgatgccg ccgaccagca
     3721 agccgagaca cgaaatggcg attgccgcga tgccgacgcc gccgatgatg ccgtcaaact
     3781 gggcgatgaa tttgtcggcg gttttgacgt cgaaattgtt tgcctcgtca aactttacgc
     3841 cgcggcgctg gcgcagaata gattcgacgt cctgcacggc gtcgtttatt tgtcccggtt
     3901 ttgcctgaat gatgtgcagg acggcgtcgc gttgcggcgc gactttctgc gccgtgcgaa
     3961 acggcagaaa aactttgcgg tcttcctcgt tttcgccgaa gaaaccggct tttcgttttt
     4021 cgataacgcc gataatttcc caggtcgtgc cgttcattcg cacgactttt ccgacgaccg
     4081 attcctgctc gttcggaaaa agcgcttcga ccgtgttgac gccgacgact atcacgtttc
     4141 ggcgctggta attgtcggat tcggtgataa agcgtccctg ccgcaaaacg aggttggtaa
     4201 cctgcatata gttcggcgtc acgccgtcgg tcagcgccca gcgataattt ctgccttcat
     4261 agacgagatt gtcgtcaaat ccgtcgcccc aggagccgat gctcggagca atcagcgcga
     4321 tgtcgtcgat ggcggctgac tgcgtcataa tcgcctgcgc gtcttcgggc gtcagcggct
     4381 tgcggttgcg ctcgtcgcgg ttgcgagcac cgggaccggt cgagaggtgg aaaacgtaga
     4441 tgttgttcgt gccgtattcc tcgacgatgg agataatcga ttggcgcatt ccggtcagaa
     4501 tcgacgccac gacaatcgcc gtcatcacgc cgacgacgat gccgagaatc gtcagagtcg
     4561 agcgaaactt attcgcataa atgctctcga aagccattcg cataatctca gccggaaatg
     4621 aagcggaaaa atttcttttc atatacgatt ctcaaaactt tttagctttg tgtcagagcc
     4681 acaatcgggt cgaggcgcgc cgctttccaa gccggataaa gcccggcgac gatgccgatg
     4741 atgctggaga cgccgacgga cataatcacg tagaaaatag tgattgtcat cgtcatttcg
     4801 agcagaaaag taatcagctg cgtgacgccc atcgccagca gcaagccgat gattccgccg
     4861 ataatgcaga gcagcgacga ttcgattaaa aactgcaaaa gaatctgctt gcgcgtcgcg
     4921 ccgagcgctt ttcgcaatcc gacctcgaaa gttctttcgg tgacggagac gagcataata
     4981 ttcatcacga cgatgccgcc gacaataagc gtaatcatcg taatcggcac gacgacggcg
     5041 gcgatggcgt tggtaaattg atccatctgc gagttaaatt cgtccacgtt gacgaggctg
     5101 aaagtgtctt cctcgctgcc gagcaagccg cgcttgtttc gcagcagcag gcgcgcttct
     5161 tcgatggc
//
LOCUS       DASWNE010001070         2401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_348178, whole genome shotgun sequence.
ACCESSION   DASWNE010001070 DASWNE010000000
VERSION     DASWNE010001070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348178"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VGB68_20420"
     CDS             <1..182
                     /locus_tag="VGB68_20420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051674.1"
                     /translation="IDFGAPSEYRISSIIDLNGDGKMEIIIYGSYYEGNWVEAYEMKG
                     NKPANIKALNAGCGV"
     gene            complement(365..1135)
                     /locus_tag="VGB68_20425"
     CDS             complement(365..1135)
                     /locus_tag="VGB68_20425"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011996181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEY0051675.1"
                     /translation="MLLVIDIGNTNTSLGVYNQENLIAHWRMNTVRDRTIDEVGVLAR
                     QMFALSEIDYKEIRAVIIASVVPPLNFTFQKMSENYFAHKAIFVDNTLDFGLKIKYNP
                     PSDVGADRIVDAVAAIAKYGMPCIVVDFGTATTFDAINADGEYLGGVITPGITISSDA
                     LFSRAAKLPRVEIKKPETVIGASTVGSIQSGLYYGYAGLVDGILRRMIEELGAETRVV
                     ATGGLAPLISQASELIAEVDDTLTLEGLRLVYEKIRKN"
     gene            complement(1230..1559)
                     /locus_tag="VGB68_20430"
     CDS             complement(1230..1559)
                     /locus_tag="VGB68_20430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051676.1"
                     /translation="MNNEFDFENEDLDFEDFEDFDQHLEDLELELEEIHTRTAVLAKN
                     NMYALLCLKTAGQGGAICRVDPREERPAVQIYDDPEKALEWFTKSLRTSKKNGWQVVY
                     DGLPLQG"
     gene            complement(1751..>2401)
                     /locus_tag="VGB68_20435"
     CDS             complement(1751..>2401)
                     /locus_tag="VGB68_20435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051677.1"
                     /translation="RALAWHTTLGGWLGAHSEIRMFNRRNSRYVTNKPLVLNLARSFG
                     LKIPDTVITNDFSYLAGFEKKRRLVVKPVNGGGYCQSFEEVRKRTELKQETAAAPAIV
                     QNRLVAPEIRVYAVGKNHFAFKITSEELDYRAAQKCEIEYLENFSDKLTVGLEKLLDA
                     LGLDFAAADFKTCPESGELVFLEVNTSPMFTVFDSASKGSLSDSMIKFLISAENLK"
BASE COUNT          617 a          605 c          582 g          597 t
ORIGIN      
        1 aaatcgactt cggcgcgccg agcgaatacc ggatttcatc aatcatcgac ctgaacggcg
       61 acggcaaaat ggaaatcatc atctacggct cgtattacga gggaaactgg gttgaagcct
      121 acgagatgaa agggaataaa ccggcgaata taaaagcgct caacgcgggc tgcggcgttt
      181 aagaattagt cacaggcgca cctggaaacc ggaagaaatt aaccacggat ttacacggat
      241 aaacccggat aaaaaaacag gatagataga aaaagcagga taaaaatcta tttcttatcc
      301 tgcttttcct tatccgtgta aatctgtgta aatctgtggt taattttctc tatgttctct
      361 gtggctaatt tttccttatc ttttcgtaaa ccagccgcaa gccttccagc gtcagcgtgt
      421 cgtcgacttc cgcaatcaat tccgaagcct gcgaaatgag cggcgcgagc ccgccggtgg
      481 cgacgacgcg cgtttccgcg ccgagttctt caatcattcg ccgcagaatt ccgtcgacca
      541 gtccggcgta gccgtaatac aaaccggatt gaatcgagcc gacggtcgat gcgccgataa
      601 ccgtttcggg ctttttaatt tctacgcgcg gaagcttggc ggcgcgcgaa aaaagcgcgt
      661 cggacgaaat cgtgatgccg ggcgtaatga cgccgcccaa gtattcgccg tcggcattaa
      721 tcgcgtcaaa ggtcgtcgcc gtgccgaaat ccacgacgat acacggcatc ccgtatttgg
      781 caatcgccgc gaccgcatcg actatgcgat ccgcgccgac gtcggacggc ggattgtatt
      841 tgattttcaa accgaaatcc agagtgttgt cgacaaaaat cgctttgtgc gcgaaataat
      901 tttcggacat tttctggaac gtgaaattca gaggcgggac gaccgaagcg atgatgacgg
      961 cgcgaatctc cttgtaatcg atttccgaaa gagcgaacat ctgccgcgcc agaacgccga
     1021 cctcgtcaat cgtccggtcg cgaaccgtgt tcatacgcca atgcgcgatt aaattttcct
     1081 gattgtaaac gccgagcgac gtgttggtgt ttccgatgtc gattacgaga agcataaaat
     1141 aatgtaaaac ggaaaacgga aaatggaaaa tgaagaaaca attcttttat tttccgtttt
     1201 ccgttttccg ttttccgcta aaaaaaaaat tagccttgca gcggcaatcc gtcgtaaacc
     1261 acctgccagc cgttcttttt gcttgtccgc aggcttttgg taaaccattc gagcgccttt
     1321 tccgggtcgt cgtaaatctg cacggcggga cgttcttcgc gcgggtcgac gcggcagatt
     1381 gcgccgccct gaccggcggt cttcaggcac aaaagcgcat acatattgtt ttttgccaga
     1441 acggcggtgc gcgtgtgaat ttcttccagc tccagctcta aatcttccag atgctggtca
     1501 aagtcttcaa aatcttcaaa atccaaatct tcgttttcaa aatcaaattc gttgttcata
     1561 tatttataca aaaagcctca attacacgtt acacgctcgc ggcgcaaagt tcaattagta
     1621 tatcgctcgt cgcgacaaag tccttaaacg gcaatccggc gccgtttgcg cggcagaaga
     1681 taaatttaga tgcgcgattt ttccgaaagt gacacgcgat tatttcaaag acaaacgatt
     1741 tcgggaaaaa tcacttcagg ttttcggcgg agattaaaaa ttttatcatc gaatcggaaa
     1801 gcgagccttt cgacgccgaa tcaaagacgg taaacatcgg ggatgtgttg acttccagaa
     1861 atacgagctc tccgctttcc gggcaggttt taaaatccgc cgccgcaaaa tcgagcccca
     1921 gcgcgtcgag cagtttttct aatccgacgg ttaatttgtc cgaaaaattt tcgagatatt
     1981 caatctcgca tttctgcgcg gcgcgataat ccagttcttc cgaagtgatt ttgaaagcga
     2041 aatgattttt tccgaccgca taaactctga tttcgggagc gacgagccga ttttgaacga
     2101 ttgccggcgc ggcggcggtt tcctgtttca gctcggttcg tttcctgact tcttcaaagc
     2161 tttggcaata gccgccgccg ttgaccggct tgacgaccag ccgccgcttt ttttcaaatc
     2221 ccgccagata ggaaaaatca ttggttataa cggtgtcggg aatttttaag ccgaaagaac
     2281 gcgcgagatt taagacaagc ggtttattgg tcacgtatct cgaattgcgc cggttaaaca
     2341 tcctgatttc cgaatgcgcg ccgagccagc cgccgagcgt cgtgtgccac gcaagcgccc
     2401 g
//
LOCUS       DASWNE010001071         1732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_632825, whole genome shotgun sequence.
ACCESSION   DASWNE010001071 DASWNE010000000
VERSION     DASWNE010001071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632825"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="VGB68_20440"
     CDS             <1..856
                     /locus_tag="VGB68_20440"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEY0051678.1"
                     /translation="RQFKEVPRMLEAFEHWFGKYPFYEDGYKLVAVSYPGMEHQSSVT
                     YGNWYRNGYRNRDVSGTGIGFKFDFIIVHESGHEWFGNNISMKDAADMWIHEGFTNYS
                     ENLFVEYHFGKKDAEDYVIGSRRNIRNDSPIIGTYGTNREGSGDMYYKGGNLLHTIRH
                     VVNDDKKWLAVMRGLNADFRHQTVTTQQIESYITNRAGIDLSKIFDQYLRTTQIPALR
                     YKTEGKTLSYKYEQVVKGFAMPVRVNINGQEVSLTPGEAMQTYTFADEIKTFEVNRNF
                     YVETEKIN"
     gene            1100..>1732
                     /locus_tag="VGB68_20445"
     CDS             1100..>1732
                     /locus_tag="VGB68_20445"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEY0051679.1"
                     /translation="MKFLDLLKEKIVVFDGAMGTNLMAQNLTLDDFGGANFENCSENL
                     IYTRPDAVLNVHRAFLEVGSDVIETNTFGGNTVVLQEFGLADKAYDVNLKAAQMAKNI
                     ANDFSTKAKPRFVAGSMGPGTKLPTLGHISYLDLKKNYEEQVRGLFDGGVDLFIVETC
                     QDILQTKAALAAIFDFFAEKRVKIPVIAQVTIETFGTMLNGTEISAALTAL"
BASE COUNT          478 a          432 c          429 g          393 t
ORIGIN      
        1 caggcagttc aaggaagttc cgcgaatgct cgaagccttc gaacactggt tcggcaaata
       61 cccgttttac gaggacggct acaagctcgt cgccgtttcc tatcccggaa tggaacatca
      121 aagctcggtg acctacggca actggtatcg aaacggctat cgaaaccggg acgtgagcgg
      181 aacgggaatc gggtttaaat tcgactttat catcgttcac gaatcgggtc acgaatggtt
      241 cggcaacaac atctcgatga aggacgccgc cgatatgtgg attcacgaag gctttacaaa
      301 ctattccgaa aacctgtttg tcgagtatca tttcgggaaa aaagacgccg aagattacgt
      361 catcggaagc cgccgcaaca ttcgaaacga cagtccgatt atcggcactt acggaacgaa
      421 ccgcgaaggt tcgggcgata tgtattacaa gggcggaaac ctgctgcata ccatccgcca
      481 cgtcgtcaac gacgataaaa aatggctcgc cgttatgcgc gggctcaacg ccgatttccg
      541 gcatcagacc gtgacgacgc agcaaatcga atcttatatt acaaatcgcg ccgggatcga
      601 tttgagcaaa attttcgacc agtatctgcg aacgactcaa attccggcgc tgcgatacaa
      661 aaccgaaggc aaaactttgt cttataaata cgagcaggtc gtcaaaggtt tcgcgatgcc
      721 ggttcgcgtc aatatcaacg ggcaggaagt atcgctcacg cccggcgaag cgatgcaaac
      781 atatactttt gcggacgaaa taaaaacttt tgaagttaat cgaaattttt atgtggaaac
      841 cgaaaaaatt aattaagccg taaaaatcgt aataactcga caatccgtaa agttttcggt
      901 aagatttaac ttccgaacgt ggtgagttac agcgacttgc gaccacgtaa aaaaatcgct
      961 aaacagctaa cgagtttcaa aacctttttt cgacaaaact ctgtgctgct ggcggcgcgg
     1021 agtttttatt ttgagaagac cgaagacgcg gttcggcgca atttgctgct ttctaccggc
     1081 taccggctac tgactactta tgaaatttct cgatttatta aaagaaaaaa tcgtcgtttt
     1141 cgacggcgcg atgggcacga atctgatggc gcagaatctg acgctggacg atttcggcgg
     1201 cgcgaatttt gaaaactgct cggaaaacct gatttacacg cgccccgacg cggttttaaa
     1261 cgtgcatcgc gcgtttctgg aagtcggctc ggacgttatc gaaaccaaca ctttcggcgg
     1321 caacacggtc gttttgcagg aattcggcct cgccgacaaa gcctacgacg tgaatctcaa
     1381 agcggcgcag atggcgaaaa atatagccaa tgatttttcg accaaagcca aaccgcgttt
     1441 cgtcgccggc tcgatgggac ccggaacaaa gctgccgacg ctcgggcata tttcatatct
     1501 cgatttgaaa aagaattacg aggaacaggt gcgcgggctt ttcgacggcg gcgttgattt
     1561 gtttatcgtc gaaacctgtc aggacatctt gcagacgaaa gccgcgctcg ccgcgatttt
     1621 cgacttcttc gccgaaaagc gcgtcaaaat tccggtcatc gcgcaggtta caatcgaaac
     1681 tttcggcacg atgctgaacg gcacggaaat ctccgccgcg ctcaccgcgc tc
//
LOCUS       DASWNE010001072         5883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_420016, whole genome shotgun sequence.
ACCESSION   DASWNE010001072 DASWNE010000000
VERSION     DASWNE010001072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420016"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VGB68_20450"
     CDS             <1..433
                     /locus_tag="VGB68_20450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051680.1"
                     /translation="ALAILLTVGLACSDSGETAATTSDNTTTVMKPESESGAVAVKSV
                     ILKNADDETVTSFKPSDLTHKASIEFSKMGAGKVRGVFTAVNAGGEKNVKILEKEIEL
                     TSQMNTANFTMKLDQPFPAGDYKLDVYLNDKLVKTHNYKVQ"
     gene            467..838
                     /locus_tag="VGB68_20455"
     CDS             467..838
                     /locus_tag="VGB68_20455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051681.1"
                     /translation="MGFFSTIGKLLSGETISFEKTTHINTAQNLLTGDRQNYIGEWRG
                     AIVNLLIKPDGAIEYRRSEVVGDTTNTDTVSGPINNFEGASFTVGVLGQNTRFEILEP
                     PHRNGNDRMTMTVNGERLEKV"
     gene            1069..2163
                     /locus_tag="VGB68_20460"
     CDS             1069..2163
                     /locus_tag="VGB68_20460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051682.1"
                     /translation="MKRQSAIIAILALVIFALGANVARAQFGDLIRKAEKKIDKTTKK
                     VNPGTSVDANGGNQNSTQTGSEGLRWDLALKNPNFVGSVNENAMKQMIAGAMPDYGNG
                     GLLVFSKQPFGKASANLDDTTLMFKSSDSIYLTAFLGAPLDISFGNALTLDALIVTVG
                     DVKTANGDYIRDTINTVYRMDKGGAKTIALDFLPAGGKSAKYQKQVGDIAASLRKLPA
                     GIHIVQLRLYGFGGLGVGAFYYDNSAGAGGTGGALDATLKSVTMPAAAKRDAALEKDI
                     TDLLNGMGGGRALRVVIVDRDWTPQRHEVTGVLIGRAIGVVVGVKSDDGRCFRENQTY
                     FQDYNGRAYTGLRLSGTREQTDMACENLTK"
     gene            2241..2609
                     /locus_tag="VGB68_20465"
     CDS             2241..2609
                     /locus_tag="VGB68_20465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051683.1"
                     /translation="MNEEKAREQKRLGVMFIAFALFQILVLLLATIAWMFLPWLGMAG
                     RSETEAMILMLAPLVGGWAFSIISILLFVLSLVAGIGVFKNSNAGRVAGIAAAYIALL
                     EIPFGTIFGVYALRRLKSPK"
     gene            2721..3272
                     /locus_tag="VGB68_20470"
     CDS             2721..3272
                     /locus_tag="VGB68_20470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051684.1"
                     /translation="MSNNSNQTARLCRSIIFTVAAIFALTFCAAAQNKTSTAHLEKEH
                     AQALRIWLRANPQYRAAQASDCKNEFGLKSMRGDDAKFHPYYAVYDFDKDGSKDFGVV
                     VIDAKRKLESQYTLLVFKGDKQRSYKTAKTIGRLDLRRGGIWLNVLEEGVTDLYVGEF
                     ETDDCFYLHWSNKKLVLRNCEGN"
     gene            3274..4260
                     /locus_tag="VGB68_20475"
     CDS             3274..4260
                     /locus_tag="VGB68_20475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051685.1"
                     /translation="MNRKIKFLSAFFIVLIGLSGNIFAQRQTPPAPKTVMDFYLLLPA
                     VKVRNLAVKTRKSFVGVEDTRNGYIKLAAPRNNDDWEAGDWMEIALFKKPNGEYVVAL
                     TNNSCTDTCAGSIEFLEYRGDRFVDVSDKIYPVTQKIKYAQYLRKKRPEQPELGESIF
                     GTVSELPRAGKTVKVKFTGEKDKELTLFEMTWNGESFVSNEPLPGAKSSPAPTSEISA
                     AEKSWSPFFTAFRRAVRKRDRAALKKMMAKNVHYDCCDQSHPSGDFRLALFERLDQNR
                     SGWRDFDAELTETFVESPTDDGLARHVGKVVFEYRAAEKRWLCTSYIAAEIP"
     gene            4510..5310
                     /locus_tag="VGB68_20480"
     CDS             4510..5310
                     /locus_tag="VGB68_20480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051686.1"
                     /translation="MKNMILFFLNGRNSRLNSTCVILILLTLILGCSDGGNPDSSGET
                     SKKLVPPAYIGVWTGQDGSTLTLRNDSTGDYKSGGKSVSGAAVEIDEAAKEIRFTLLG
                     FDSGKYKIDQPPAGNKMKLDGMEYRRTGGFDVGDSEKTSDATKTGTIPTEEMLRPIAS
                     ETIRDFDAAVQQGDFSDFYSTVSEMWQSQMTSADLDKAFAPLAEQKNDYKLKTDAPLS
                     FSSKPTVKDEVLEINGSYQNVKGKNIAFRLRYVEENNVWKLLGIRLNP"
     gene            5425..>5883
                     /locus_tag="VGB68_20485"
     CDS             5425..>5883
                     /locus_tag="VGB68_20485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051687.1"
                     /translation="MKLSKLILIAICAFALTFAACGGASTETSKTNTTNAGNTATTTQ
                     TKTETATKTAAVKQSTPSEAGESFINAVKAKDKTAFRQLMSKDSMEILNLAAREKKMT
                     VDDLLDKEFFVNAEMPAKLEQRNEKITGDKATIEMQDDKGEWSPMTFVKEG"
BASE COUNT         1671 a         1421 c         1531 g         1260 t
ORIGIN      
        1 agctttagcg attttattaa ccgtcggctt agcgtgcagc gacagcggcg aaactgcggc
       61 gaccaccagt gacaacacga caactgtgat gaaacccgaa tcggaaagcg gcgctgtggc
      121 ggtcaaatcc gtaatcttga agaacgccga cgatgaaacc gtgacgagct ttaagccgtc
      181 ggatttgacg cacaaagcct cgattgaatt ctctaaaatg ggcgctggca aagtcagagg
      241 cgttttcacg gcagtcaacg cgggcggaga gaaaaacgtg aagattctcg aaaaggaaat
      301 cgagctgact tcccagatga acacggctaa tttcacgatg aagctcgacc agccgtttcc
      361 cgccggcgac tacaaactgg acgtttatct gaacgacaag ctcgtcaaaa ctcataacta
      421 caaagttcaa taaaaattaa aaaggaaaat taaggagaaa aagaaaatgg gatttttcag
      481 cacaatcggc aagctgcttt cgggcgaaac gatttctttc gagaaaacta cgcacattaa
      541 tacggcgcag aatttattga ccggcgatag gcaaaactat atcggcgaat ggcgcggggc
      601 gatcgtgaat cttttaatca agccggacgg cgcgattgaa tatcggcgct cggaagtggt
      661 cggcgacacg acgaacaccg acactgtttc gggaccaatc aataattttg aaggcgcgag
      721 ttttacggtc ggcgttctgg gacaaaacac gcggtttgaa atccttgagc cgccgcaccg
      781 gaacggcaac gacagaatga caatgacggt caacggcgag cggctcgaaa aagtttagac
      841 caggaagcgt ttgaatggca ttcgttaaat aaacgggctg gaaatttaca aaattcaaat
      901 ttacctaagc gggattggtt cccgcttttt gctgcccgaa aaaaaatatt ttcgctctgg
      961 taatatgccg ccggtttcgc gcctcttcgg taatcaaagg cgggcgaacg aatttaagat
     1021 aaatgcgccg ccgttcaaag tttaagcaaa ttaaaaggaa aaaccaatat gaaacgacaa
     1081 tcggcaatca tcgcaatttt agcattagtc atcttcgcgc tcggcgcaaa cgtcgcccgc
     1141 gcgcagttcg gcgatttaat caggaaggcg gagaaaaaaa tcgataaaac gacgaaaaaa
     1201 gtaaatcccg gcacgagcgt cgacgcaaac ggcggcaatc aaaattcaac gcaaaccggc
     1261 agcgaaggct tgcgctggga tttggcgtta aaaaatccga actttgtcgg cagcgtgaac
     1321 gaaaacgcga tgaaacagat gattgcgggc gcaatgcccg attacggcaa cggcggtttg
     1381 ctggttttct cgaaacagcc gttcggcaaa gcttcggcga atttggacga cacgaccttg
     1441 atgttcaaat cgagcgattc gatttacctg acggcgtttt taggcgcgcc gctcgacatt
     1501 tctttcggca acgcgctgac gctcgacgca ttgattgtga cggtcggcga tgtaaaaacg
     1561 gcgaacggcg attacatcag agatacgatt aacaccgtct atcgaatgga caaaggcggc
     1621 gcgaaaacga ttgcgctcga ctttctgccc gcgggcggaa aatcggcgaa atatcaaaaa
     1681 caggtcggcg acatagccgc gagcctgaga aaactgcccg ccggaatcca catcgttcag
     1741 cttcgtcttt acggtttcgg cggtctggga gtcggcgcgt tttattacga caattcggcg
     1801 ggcgcgggcg gcacaggcgg cgcgctcgac gctactttga aaagcgtgac gatgcccgcg
     1861 gcggcgaaac gcgatgcggc gctcgaaaaa gacatcaccg atttattaaa cggaatgggc
     1921 ggcggacgcg cgctgcgggt ggtgattgtc gaccgcgatt ggacgcctca gcgccacgaa
     1981 gtaaccggcg ttttaatcgg gcgcgccatc ggcgtcgtcg tcggcgtcaa atcggacgac
     2041 ggcagatgtt tccgcgaaaa tcaaacttat tttcaggatt acaacgggcg cgcatacacc
     2101 gggctgcgcc tgagcggaac gcgcgagcaa acggatatgg cttgcgaaaa tctgacgaag
     2161 taaatcgaat tactttgaaa atcgggcgct gaatcgaccg cgcccgattt tcaaaacaaa
     2221 caaaacccgg agaaaaatca atgaacgaag aaaaagcccg tgagcaaaaa cggctcggcg
     2281 taatgtttat cgctttcgcg ctgtttcaaa tccttgtttt gctgctcgcg acgatagcgt
     2341 ggatgtttct gccgtggctc ggaatggcgg ggcgcagcga aacggaagcg atgattttga
     2401 tgctcgcgcc gctcgtcggc ggctgggcgt tttcgattat ctcgatttta ctgttcgtgc
     2461 tgtcgctggt cgccggaatc ggcgttttca aaaacagtaa cgcggggcgc gtcgccggaa
     2521 ttgccgccgc ttatatcgca ttactcgaaa ttccgttcgg gacaatcttc ggcgtctacg
     2581 cgctgcgccg tcttaaatcg ccgaaatagc cgcgccgcgc gacaggaaga atcataaatt
     2641 ataaaaatcc gaattttcta tcgctctcga taatacatcg cttgtttacg gctcgcgttc
     2701 gagcagaaat tataaaaatt atgtcaaaca attcaaatca aaccgcgcgc ctttgtcgct
     2761 cgataatttt caccgtcgcc gcgattttcg cgctgacctt ttgcgcggcg gcgcagaata
     2821 aaacttcgac ggcgcatttg gaaaaagaac acgcccaagc gctgcgaatt tggctcagag
     2881 cgaatccgca atatcgcgcc gcgcaagcgt ccgactgcaa aaacgaattc ggtctgaaaa
     2941 gtatgcgcgg cgacgatgcg aaatttcacc cgtattacgc ggtttatgat ttcgacaaag
     3001 acggaagcaa agatttcggc gtcgtagtca tcgacgcgaa acgcaaactc gaatctcaat
     3061 acacgctgct cgtcttcaaa ggcgacaaac aaagaagtta caaaaccgcg aaaacgattg
     3121 gcagactgga tttgcggcga ggcggaatct ggctcaatgt gctggaagaa ggcgtcaccg
     3181 atttgtacgt cggcgaattt gaaaccgacg actgctttta cctacactgg agcaacaaaa
     3241 aactggtttt gagaaattgc gagggaaact aaaatgaatc gaaaaataaa atttttatca
     3301 gcgtttttta ttgtattgat tggcttgagc ggaaacatct tcgcgcaaag gcaaacaccg
     3361 ccggcgccga aaaccgtgat ggatttttac ctgcttttgc ccgccgtaaa agtcagaaat
     3421 ctggcggtta aaaccagaaa atccttcgtt ggcgtcgaag acacgcgcaa cggttacatc
     3481 aagctcgccg cgccgcgaaa taacgacgac tgggaagcgg gcgattggat ggaaatcgcg
     3541 ctgttcaaaa aaccaaacgg cgaatacgtc gtcgctttaa caaataacag ctgcaccgat
     3601 acttgcgcgg gcagcataga gtttttggaa tatcgcggcg accgcttcgt cgacgtcagc
     3661 gataaaattt atccggttac tcaaaaaatc aaatacgcgc aatatcttcg caaaaaacgt
     3721 cccgaacagc ccgaattggg cgaatcgatt ttcggcacgg tcagcgaatt gccgcgcgcg
     3781 ggtaaaaccg tcaaagtcaa attcacgggc gagaaagaca aagaattaac gttgtttgaa
     3841 atgacctgga acggcgaaag cttcgtctcg aatgaacctt tgcccggagc gaaatcgtcg
     3901 cccgcgccta ccagcgaaat ttcggcggcg gaaaaatcct ggtcgccgtt cttcacagcg
     3961 tttcgccgag ccgtccggaa acgcgaccgc gccgcgttaa aaaagatgat ggcgaaaaac
     4021 gttcattacg attgctgcga ccaatcgcat ccgtcgggcg attttcggct cgcccttttt
     4081 gagcggctcg accagaaccg cagcggctgg cgcgatttcg acgcggagct gaccgaaaca
     4141 ttcgtcgaaa gtcctacgga cgatggcttg gcgcgccacg tcggcaaagt cgtatttgaa
     4201 tatcgcgccg ccgagaaacg ctggctttgc acttcgtata tcgcggcgga aataccgtag
     4261 aaatgagcga cgattgatgt tcggaaaaat gtttccggac aaccgttcaa atctcaagag
     4321 cgaaacgtct tcgctctttt ttattttccg aaaaaatctc cggtcgcggt aatacgtcga
     4381 aattctctga ctctataacc tcgagcgcgg caaatatttt caaaagcgcc gccggtttat
     4441 ttactgaaac aggagagaaa tcctaggatt gtttacgttt ttcgggcagg aaatatggag
     4501 tgtaaattta tgaaaaatat gattttattt tttttgaacg gacgaaattc acgattgaat
     4561 tcgacgtgcg tcatcttaat tttattgacg ctgattttag gctgcagtga cggcggcaac
     4621 ccggattcga gcggcgaaac gtcgaaaaag cttgttccgc ccgcttatat cggcgtttgg
     4681 acgggacagg acggctcgac cttaacgctg cgcaacgaca gcaccggcga ttacaaatcc
     4741 ggcggcaaat ccgtcagcgg cgcggcggtg gaaatcgacg aggcggcaaa ggaaattcgg
     4801 tttacgcttc tcggtttcga ttcgggcaaa tacaaaatcg accagccgcc ggcgggaaat
     4861 aaaatgaagc tcgacggaat ggaatatcga cgcaccggcg gttttgacgt cggcgattct
     4921 gaaaaaacat cggacgcgac gaaaacggga acgattccga cggaagagat gctgcgcccg
     4981 attgcctcgg aaacgattcg agattttgac gcggcggtgc agcaaggcga tttcagcgat
     5041 ttttattcga ccgtctcgga aatgtggcaa agccagatga cctccgccga cctcgacaaa
     5101 gcgtttgcgc cgctggcgga gcagaaaaac gattacaaat tgaaaacgga cgcgccgctg
     5161 tctttttcgt cgaagccgac cgttaaagac gaagttttag aaatcaatgg cagctaccaa
     5221 aacgtcaaag gcaaaaatat cgcttttcgc ctgcgatacg ttgaagaaaa caacgtctgg
     5281 aaactgctcg gcattcgtct taatccgtaa acgatattag cacgaatcga aaaataattt
     5341 ttcgattcgt gccaatatcg tttgtttttc cgactctatc tcattgaagc cgaaaataaa
     5401 cggcaatttt acgggagaaa aattatgaaa ttatcaaaat taattttaat cgcaatttgc
     5461 gctttcgcac tgactttcgc cgcgtgcggc ggcgcttcaa ccgaaacttc taaaaccaat
     5521 acaaccaatg cgggaaatac tgcgacgaca acgcaaacca aaacggaaac ggcgaccaaa
     5581 accgcagccg tcaaacaatc gacgccgagc gaagcgggcg aatcgtttat caatgccgtg
     5641 aaggcgaaag acaaaactgc gttcaggcaa ttgatgtcaa aagattccat ggaaattctg
     5701 aatttggcgg cgcgggaaaa gaaaatgacg gtggatgacc ttctcgacaa agagtttttc
     5761 gtcaacgcgg aaatgcccgc caaactcgaa caacgcaacg aaaaaatcac gggcgacaaa
     5821 gcgacaattg aaatgcagga cgacaaaggc gaatggtcgc cgatgacttt tgtcaaagaa
     5881 ggc
//
LOCUS       DASWNE010001073         3054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_633606, whole genome shotgun sequence.
ACCESSION   DASWNE010001073 DASWNE010000000
VERSION     DASWNE010001073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633606"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="VGB68_20490"
     CDS             complement(<1..977)
                     /locus_tag="VGB68_20490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051688.1"
                     /translation="MRDIPVGNGSLLVTFDEKYQIRDVYFPHVGQENHTAGYPFRFGV
                     WADGDFSWISEGIWKRRLRYQKETLVTDVHLINDRLGLEIVSHDTVASHENIFLRCVR
                     VSNLRDAERDVRVFLHHDFRISENKVGDTAFYDPETFALVHYKRDRYFLVNTEPHFDS
                     FVTGRKDFRDQEGTWRDAEDGVLQGGAITEGSVDSTLGIHLNLGAQQTREFFYWIAAG
                     TSHEEVAALNQLVKEQTPNKYLGYTENYWRVWVNKNDIDFADLTPEIVELFKRSLLII
                     RTQADNDGAILAANDSDVTERATDHYSYLWTRDGALVANALDLAGFPDIA"
     gene            complement(1128..1760)
                     /locus_tag="VGB68_20495"
     CDS             complement(1128..1760)
                     /locus_tag="VGB68_20495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051689.1"
                     /translation="MKKMKTLSLLLKLTLCLMIVFGVHSNADAVTAIATVGPNTSAPN
                     VFFTGVCNGTFTLATNNAFIRSKVGFGFNPNVAVTFGPMVGLCPTYQMSPNGIWHLVL
                     ADPNGNPVPFSVTDNINGNILLQGTFGRAILHGRSGNSSLALTLWQDNVTYNAASLWF
                     PNGGFPMTNGSLSVAILAMQPVIANPPNDDGGAPGPAAFQANGDINFGRQ"
     gene            1901..>3054
                     /locus_tag="VGB68_20500"
     CDS             1901..>3054
                     /locus_tag="VGB68_20500"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY0051690.1"
                     /translation="MSRKIKHFYEFGEFRLDAETPSLWRDGRLVQIFPKALEVLVMLV
                     EKRDAVVSREELLEKIWQDAFVEESNITYTVSLLRKTLNENGKKHFIQTVPKRGYRFV
                     ADVREVVENGAVETKSVPLDSAAVVSIPIEPSIPIEQLKAQQEPKAPAEPKAAEEPKA
                     QDEPKAREEPKTQVRWHFISIILLGLLFVTSFAVWWSFGDRRGLSSVAVSERNIRTIA
                     ILPLKTLTESEQSTALSLGLTDSLISRLGSLNRFAVRPLNSVKDYTEAGKNPLEFGEE
                     LKVDAVLEGTLQIVENRLRVNLRLWDVRDGAQLWQGSFDSAEADFFNLQDTISTKVTQ
                     SLVSRLIEKDRRLLTRRETENRDAFHAYWRGRFFLEKRNPAKAIAEFQQA"
BASE COUNT          724 a          785 c          836 g          709 t
ORIGIN      
        1 gcgatgtccg ggaagccggc caggtcgagc gcgttggcga ccagcgcgcc gtcgcgcgtc
       61 cagagatagc tgtaatggtc ggtcgctctt tcggtcacgt ccgaatcgtt agcggcgaga
      121 attgcgccgt cattgtccgc ctgcgtgcga ataataagca gcgaacgctt aaataactcg
      181 acgatttccg gcgtcaggtc cgcaaaatcg atgtcgtttt tattgaccca gacgcgccag
      241 taattttccg tgtagccgag atacttgttc ggcgtctgct ctttgaccag ctgattcagc
      301 gcggcgactt cttcgtgcga agtgcccgcc gcaatccagt aaaaaaactc gcgcgtttgc
      361 tgcgctccga gattcaggtg aattccaagc gtcgaatcga ccgagccttc cgttatcgct
      421 ccgccctgca gaacgccgtc ttcggcgtcg cgccacgttc cttcctggtc gcgaaaatct
      481 tttctgcccg tcacgaacga atcgaaatgc ggctcggtat taaccagaaa atagcggtcg
      541 cgcttgtaat gaacgagcgc gaaagtttcc gggtcgtaaa aggctgtgtc gccgaccttg
      601 ttttccgaga tgcgaaaatc gtgatgcaga aaaacgcgaa cgtcgcgctc cgcatcgcgc
      661 agattgctga cgcggacgca acgcagaaaa atattttcgt ggctcgccac cgtatcgtgc
      721 gaaacgattt ccaagccgag ccggtcgttg attaagtgaa cgtcggtgac gagcgtttcc
      781 ttctggtaac gcagtcggcg tttccagatg ccttcggaaa tccacgaaaa atcgccgtcc
      841 gcccagacgc cgaagcgaaa cgggtagccc gccgtgtggt tttcctgccc gacgtgcgga
      901 aaataaacgt cgcggatttg atatttttcg tcaaaagtta ctaaaagaga gccgttgccg
      961 acgggaatat cgcgcataaa attacgagaa aaagtttcgt aattatttta cacgattttt
     1021 aagcgaaagc attgtgagta aatgattaaa gcgcaggcgc tcgcggcttg cgctttaatc
     1081 gtttgttccg gtctgttaaa cgaacggttt tggctgcggc agcgcgctta ttgcctgccg
     1141 aagttgatgt cgccgtttgc ttgaaacgcc gccggacccg gcgcgccgcc gtcgtcgttg
     1201 ggcggattgg cgatgacggg ctgcatcgcc aggatggcga ccgataaaga gccgttggtc
     1261 atcgggaagc cgccgttggg aaaccagagc gacgcggcat tataagtcac gttgtcctgc
     1321 cacagggtca aagccagcga gctgtttccc gaacgaccgt gcagaatcgc tctgccgaaa
     1381 gtgccttgca gcaaaatatt cccgttgata ttgtcagtca ccgaaaacgg aaccgggttt
     1441 ccgttcgggt cggcgagaac cagatgccag atgccgttcg ggctcatctg gtaagtggga
     1501 cagagcccga ccatcggacc gaacgtcaca gctacattgg gattgaagcc gaagccgacc
     1561 ttgcttcgga taaaagcatt gtttgtggcg agcgtaaagg tgccgttaca gacgcccgta
     1621 aagaagacgt tcggcgccga cgtgttcggt ccgaccgtgg cgatagccgt gacggcgtcg
     1681 gcattcgagt gaacgccgaa aacaatcatc agacatagtg ttaatttcag cagtaagctt
     1741 agggttttca tttttttcat ttttttacct ttttatcttt tgagtttttt gtcatcagcg
     1801 caaaaaattt gcgtttgatg cgtcaacagt tttacccttt agtcaagaaa aaagcctttg
     1861 gaaaagattg gaaatttgtt ggaaaaacat ttgattgccc atgtctcgca aaatcaaaca
     1921 tttttacgaa tttggcgagt ttcgtctcga cgcggagacg ccgagcctgt ggcgcgacgg
     1981 caggctggtt cagatatttc cgaaggcgct cgaagtttta gtgatgctgg tcgaaaagcg
     2041 cgacgccgta gtctcgcgtg aagaattgct ggaaaaaatc tggcaggatg cttttgtcga
     2101 agaaagcaat atcacttaca ccgtctcact gctcagaaaa actctcaacg aaaacggcaa
     2161 aaagcatttt attcaaaccg tgccgaaacg cggctatcgt tttgtcgccg acgtgcgcga
     2221 agtcgtcgaa aacggggcgg tcgaaaccaa atccgttccg ctcgattcgg ctgcggtcgt
     2281 ttcaattcca atcgagcctt caattccaat cgagcagttg aaagcgcagc aagagccgaa
     2341 agcgcctgca gagccgaaag cggcggaaga gccgaaagcc caggatgagc cgaaagcccg
     2401 cgaagagccg aaaacgcagg ttcgctggca ctttatatcg ataattttgc tcggcttgct
     2461 ttttgtgacg agcttcgccg tctggtggag tttcggcgac cggcgaggac tttcgagcgt
     2521 tgccgtcagc gagagaaata tccgcacaat cgctattttg cctctgaaaa cattgaccga
     2581 aagcgagcaa agcacagccc tgtcgctcgg tttgaccgat tcgctgatta gccgcctggg
     2641 cagcctgaat cgttttgccg tgcgcccgct caactcggtc aaagattaca cggaagccgg
     2701 caaaaacccg ctcgaattcg gcgaagaatt aaaggtcgat gccgttttgg aaggaactct
     2761 gcaaatagtc gagaatcgcc tgcgggtcaa tctgcgctta tgggacgtgc gcgacggcgc
     2821 gcagctctgg caaggctcgt tcgactcggc ggaagcggat tttttcaatc ttcaggacac
     2881 gatttcaact aaagttacgc aatcgctcgt ttcgcgtttg attgaaaaag accgccgcct
     2941 tttaacccgc cgcgagacgg aaaaccgcga cgcttttcac gcctactggc gcgggcgttt
     3001 ttttctggag aaaagaaatc ccgccaaagc gattgccgaa tttcagcagg cgat
//
LOCUS       DASWNE010001074         4096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_420711, whole genome shotgun sequence.
ACCESSION   DASWNE010001074 DASWNE010000000
VERSION     DASWNE010001074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420711"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            277..795
                     /locus_tag="VGB68_20505"
     CDS             277..795
                     /locus_tag="VGB68_20505"
                     /inference="COORDINATES: protein motif:HMM:NF027592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HEY0051691.1"
                     /translation="MNKFLGLIILTFALSLAAFAQKKTESKPTDADKQATFDAAGMIK
                     RGAALSGKAEKVSLAKVLENPSKYAGKTVQVEGVIVRSCKMEGCWAELAPDAKSKSVR
                     VKMKDHAFFIPLQSAGFKARADGVFTIKTLTKEQVDHMIEEDGAKFETRNADGTVTEI
                     SFEAAGIELRKS"
     gene            complement(941..1483)
                     /locus_tag="VGB68_20510"
     CDS             complement(941..1483)
                     /locus_tag="VGB68_20510"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY0051692.1"
                     /translation="MEKKNGPWTIKETRRIYKNEFLELFEDKVVQPDGKDGEYATVEM
                     KAGVCTLAIDEENNVYLTRQFRYALGAESLEVIAGGIDEGEPLENAKREAREEMGIEA
                     DEWIDLGTAETDTSIVRSTAHFFVARKLKFTEPDREGTEAMKPAKMRFDQAVEKVLSG
                     EIKHALSCLLILKAKLKLRD"
     gene            1547..2437
                     /locus_tag="VGB68_20515"
     CDS             1547..2437
                     /locus_tag="VGB68_20515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051693.1"
                     /translation="MRVNFSIIIFLILLTFCSTENAFADGALGGKLLKFNGKPLPYTE
                     IELVPVDSAKQVADRKLWATTAANGNFTFDKIPPGEYTLSINFGEKPTDLSPYATYFY
                     PKASNRALAKIFEIYADTRIANLIFQLSPPLAKRKILGRVFGNDGAPAANVYVALRDV
                     EGDKEDALLGFSPIKTDKGGNFSLMSFEMRKYQIFAILLQGSDKAPSFDPLAKLVAAA
                     KSDVFVLDAKTGSITLRLVSIGDSDKPPENTETEGGASARFKKNVNEETKSFEVESEP
                     FTFNNNVSNIKLSLAAKKEN"
     gene            2458..3549
                     /gene="dusB"
                     /locus_tag="VGB68_20520"
     CDS             2458..3549
                     /gene="dusB"
                     /locus_tag="VGB68_20520"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEY0051694.1"
                     /translation="MKAFKIRNIEINPPLILSPMAGVTDYTFRRLIKRRGGVGMVVSE
                     FISVEGLTRHNPKSKRQMRFDEEERPFAVQIFGGQPGRMALGAEMAEEVGADILDVNC
                     GCPAPKVVKHGGGSALLKDLPRLEIILKEIKKSISIPLTLKMRTGFSDSTINCVEAAK
                     MAEQCGVEHIQVHGRTREQGYKGMANWDLIRAVKEAVSIPVSGNGDITTIEYGLKRWR
                     ETNCDGILIGRGAMQNPWIFRQFQDAMEGREPYQPDLAEKKAVLLEFFEMLREEMPEI
                     VALGKMKQLAGQFTKGLVGGAQFRQTLYHSHSAQEILDNITVYFETLSERATFGDGLT
                     EADADKDLAIDSCEAFSNEETSCNAAVAN"
     gene            3617..4096
                     /locus_tag="VGB68_20525"
     CDS             3617..4096
                     /locus_tag="VGB68_20525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051695.1"
                     /translation="MRFIKLKKISVTFVFLLISALAGSAQTNSKPKPAQTPLNVTGSA
                     QAAIKSSPAYAEVLLRKTELEATLDDLVFQFTDEFPKVKETRFELGAIRRELDRILAV
                     NDASKLSQGLGRLIVRKVELETDLWSLRQKYNDEHDDVKRALRKVAAFEKAIKEILP"
BASE COUNT         1072 a         1020 c         1028 g          976 t
ORIGIN      
        1 ggcttgtttt ccaagtaacc ggcgaaataa aatctccgcc gcagacgttt gcccgaaacg
       61 agcgggcgtt tgataaaagc accgacgcct tgcctcgcgc cggattcgtg tgttaaaaat
      121 ctaaacaaat ttttaacatt ggagctttaa aggcagaacg tcgactcgac agatattgcc
      181 cactttactg tatactcgtt caaggtcgtg aaccggacgg ttatcaccgg cttgccgaac
      241 agaccggaca ttcaataatt tataaggagc aaaaaaatga ataaattttt aggattgatt
      301 attttaactt tcgctttatc gctggcggct ttcgcacaga aaaaaacgga gtccaaaccg
      361 accgacgccg acaaacaggc gactttcgac gccgccggga tgattaagcg cggcgcggcg
      421 ctttccggca aagccgaaaa agtgtcgctg gctaaagtgc tggaaaatcc ttcgaaatac
      481 gccggcaaaa ccgtgcaggt cgagggcgta atcgtgcgtt cgtgcaagat ggaaggctgc
      541 tgggctgagc tggcgcccga cgccaaatca aaatcggtgc gcgtcaaaat gaaagaccac
      601 gcctttttta tcccgcttca atccgccggg ttcaaagcca gagccgacgg cgtttttacc
      661 atcaaaaccc tgaccaaaga acaggtcgac catatgattg aagaagacgg cgcgaaattc
      721 gaaacgcgca acgccgacgg aacggtgacg gaaatctcgt ttgaagcagc gggcatcgag
      781 ctgcggaaaa gctgattttt agcctcttgg caacacgaaa agacacgaac agctcgccgt
      841 gataacaata cagacaagga ttttttaaat ttatttctta tccttatcca tcctgtcagc
      901 acggcgaatt cttatgcgtc ttttcgtatt cctttcgtaa ttaatctctt aatttcagct
      961 tcgctttcaa aatcaaaaga cagctcagcg cgtgtttgat ttcgccgctc agaacttttt
     1021 cgaccgcctg gtcaaatctc attttcgcgg gcttcatcgc ttccgttccc tcacggtcgg
     1081 gttcggtgaa tttcagcttt cgggcgacga aaaaatgcgc cgtcgaacga acgattgacg
     1141 tatcggtttc cgccgttccc aaatcaatcc attcatcggc ttcgatgccc atttcttcgc
     1201 gcgcttcgcg tttcgcgttt tcgagcggct cgccctcgtc tatgccgccg gcgattactt
     1261 ccaggctttc ggcgccgaga gcgtagcgaa actgccgcgt caaataaaca ttattttcct
     1321 cgtcaatcgc cagcgtgcag acgcccgctt tcatctcgac ggtcgcgtat tcgccgtcct
     1381 tgccgtccgg ctgaacgact ttatcttcaa acagttcgag aaattcgttt ttataaatcc
     1441 gccgcgtttc cttaatcgtc cacggtccgt ttttcttttc cataattacc ggattattgt
     1501 aacatctctg tcgggcgaaa aaatttataa aatcgctcga agcattatga gagttaattt
     1561 ttcaatcata atttttttaa ttcttctcac attctgttcg accgaaaatg catttgccga
     1621 cggcgcgctc ggcggcaagc ttttaaaatt taacggaaaa ccgctgcctt acacggaaat
     1681 agagcttgtc ccggtcgatt cagccaaaca ggtcgccgat agaaagctct gggcgacgac
     1741 cgccgccaac ggcaatttta ctttcgacaa aatcccgccg ggcgaataca ctctgagcat
     1801 caatttcggc gaaaagccga ccgatttatc gccgtatgcg acttattttt acccgaaagc
     1861 ctcaaatcgg gcgctggcga aaatttttga aatctacgcc gatacgcgca ttgcgaattt
     1921 gatttttcag ctttcgccgc cgctcgccaa acgaaaaatc ctgggcagag tattcggcaa
     1981 cgacggcgcg ccggcggcaa acgtctacgt cgctctgcgc gacgtcgaag gcgataaaga
     2041 agacgcttta ctgggtttct cgccaatcaa aaccgataaa ggcggaaatt tttcgctgat
     2101 gagctttgaa atgagaaaat accagatttt tgcgatattg ctgcagggca gcgataaagc
     2161 cccgtctttc gacccgctcg ccaagctcgt cgcggcggcg aaatccgacg ttttcgtcct
     2221 cgacgctaaa accggaagca taacactaag gcttgtgagc ataggcgatt ccgataagcc
     2281 gccggaaaat acggaaacgg aaggcggcgc ttcagcccgg tttaaaaaaa atgtaaacga
     2341 ggaaactaaa tcgtttgaag ttgaatcaga accgtttacg tttaacaata atgtttcaaa
     2401 cataaaatta tcgctcgcgg cgaaaaagga aaattaaatt atagttttca gaaacaaatg
     2461 aaagctttca aaatacgaaa catcgaaatc aatccgcctt tgattctctc gccgatggcg
     2521 ggcgtgacgg attatacttt tcgccgtctt atcaagcggc gcggcggcgt cggcatggtc
     2581 gtgtcggagt ttatctcggt cgaaggcttg acgcgccaca atccgaaatc gaaacggcag
     2641 atgcgttttg acgaggaaga acgcccgttt gccgtgcaga ttttcggcgg gcagcccgga
     2701 cgaatggcgc tcggcgcgga aatggcggaa gaagtcggcg ccgatattct cgacgtcaac
     2761 tgcggctgtc ccgcgccgaa agtcgtcaaa cacggcggcg gctcggcgct tttaaaagat
     2821 ttgccgcgtc tggaaattat cctgaaggaa atcaaaaaat caatctcgat tccgctgact
     2881 ttgaaaatgc ggacgggctt ttccgattcg accatcaatt gtgtcgaagc ggcgaaaatg
     2941 gcggagcaat gcggcgtcga gcacattcag gttcacgggc ggacgcgcga gcagggttat
     3001 aagggaatgg caaactggga tttgattcgc gccgtgaaag aagccgtttc gattccggtt
     3061 tccggcaacg gcgacatcac gacgattgaa tacggcttga aaagatggcg cgagaccaat
     3121 tgcgacggaa ttttaatcgg gcgcggcgcg atgcagaatc cctggatttt ccgccagttt
     3181 caggacgcga tggaagggcg cgaaccgtat cagccggatt tggcggaaaa gaaagccgtt
     3241 ttgctcgaat tttttgaaat gctccgcgag gagatgccgg aaatcgtcgc tctggggaaa
     3301 atgaaacagc ttgccggaca atttacgaaa ggcttggtcg gcggcgcgca gtttcgccag
     3361 acgctctatc attcgcattc ggcgcaggaa attctggaca atatcacggt gtacttcgaa
     3421 actttgtcag agcgagcgac tttcggcgac ggcttgaccg aggctgatgc ggacaaggat
     3481 ttggcgattg attcgtgcga agcgttttca aacgaagaaa cttcctgtaa tgcggcggtc
     3541 gctaattaga attttccaaa cactggttta aacgcaattt tgaggcggaa aatttccccg
     3601 ccccggacaa actgaaatga gatttatcaa attaaaaaaa atttccgtaa cttttgtttt
     3661 tcttttaatt tccgctcttg ccggctcggc tcagacgaac agtaaaccga aaccggcgca
     3721 gacgccttta aacgtcacgg gcagcgcgca ggcggcgatt aaatcttcgc ccgcttacgc
     3781 cgaagttctc ttgcgcaaaa ccgagctcga agcgacgctc gacgatttag tgttccaatt
     3841 caccgacgaa tttccgaaag tcaaggaaac acgcttcgag ctgggagcga ttcgcaggga
     3901 attagaccgg attctggcgg ttaacgatgc gtcgaaattg agtcagggtc tgggtagatt
     3961 aatcgtgcgc aaagtcgagc tggaaaccga cctctggagt ttgcggcaaa aatacaacga
     4021 cgaacacgac gacgtgaagc gcgccctgcg caaagtcgcg gcgtttgaaa aagcgattaa
     4081 agaaattttg ccgtaa
//
LOCUS       DASWNE010001075         4997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_349443, whole genome shotgun sequence.
ACCESSION   DASWNE010001075 DASWNE010000000
VERSION     DASWNE010001075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349443"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..412)
                     /locus_tag="VGB68_20530"
     CDS             complement(92..412)
                     /locus_tag="VGB68_20530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004946303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEY0051696.1"
                     /translation="MFGTNEILWTIISWIIFGLIVGLIARFLMASRHTGGMLTTILLG
                     IGGAIVGGVIGRFIFGFGFRTGNSPEDFTLPSFLLNLLCAIVGAIALSAAYRIFLDKS
                     TADG"
     gene            complement(471..3065)
                     /gene="mutS"
                     /locus_tag="VGB68_20535"
     CDS             complement(471..3065)
                     /gene="mutS"
                     /locus_tag="VGB68_20535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEY0051697.1"
                     /translation="MKAETPIRRQYLEIKAKHRDAILLFRLGDFYEAFDEDAVLLAKE
                     LDIVLTSRSMGKDERVPLAGVPHFVLERHLATLIARGHRVAICEQTSSAPVKGKDGKK
                     LIQREVVRIVTAGTVIEPSLLESKTNNYLCAFVSDGLRAGIAYADVTTGDFAATEIEN
                     SDAITELQRLAPAEIILADAEETFSNQNLPTFVTRLNAEYFSLETARKTLFNHFSTKT
                     LQPFGLEKSPLATSAAGALILYLNESQSGTAEQLTRLTSYDSRDFMLIDAHTLRSLEI
                     FESAADASLLSVIDRTKTAMGGRLLRRWLRQPLLDTAEIYKRQEHIAWFKDNPHERRE
                     LAYIFSEMKDLERLSSRARANFISAYELRAFGKSLELIPKIKQILQNDTVRFNQLLAH
                     LPDCSPTAKLIDSAISEELPNRHVEQVGIIREGFSEELDKLRATLYDGKGFLAEMEKR
                     ERERTGIKSLRVGYNKVFGYYIEVTKSNLHLVPSNYVRKQTLIPAERYITLELKEHES
                     LVVHAKERIAELEHSLFRQVCGEIGKARPQILLAASTIAYLDAITGLADAADEMNYSR
                     PTVNETSLLRIKNGRHAVVEKILREENVRFVANDSALGGNGAPNIALITGPNASGKST
                     YLRQIALIVLLAQIGSFVPAEDAVVGLCDRIFTRIGLYDRIGAGESTFMTEMIETAEI
                     LHHATPRSLILLDELGRGTSTFDGLAVARAVLEFVHNHPKLKTRTLFATHYHELAELE
                     GVLPHLENLHVEIEESNGELTFKYKISRGVALKSYGVYAARLAGLPNPVIRRAEELLK
                     EYEGKVESDEPGVASPLDDELRKKLSEIDTDSLSPVEALMKLYELKDIAKQEKKVENK
                     RHLKRAIG"
     gene            complement(3201..3902)
                     /locus_tag="VGB68_20540"
     CDS             complement(3201..3902)
                     /locus_tag="VGB68_20540"
                     /inference="COORDINATES: protein motif:HMM:NF025696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY0051698.1"
                     /translation="MPFPKSSSIEMPVLQELYAIGGAEDVRFLYERLTAYFPQLSETE
                     IREIRNGTKANWRKLIQKAGKKLDEKGLLERNRGFWNLTEKGKRTVASESEGFQVSKP
                     EQKELNHREIQEMLVEIGASLGYDAQTEFEYYDVVWREKRSAQRLSHIFEVQSKGNID
                     SAFAKLKRAHDAQRTKPFLVLASERDTNRARQSLEREFHELADVLTILSFAELERIHR
                     NLRAIAEILPKFLER"
     gene            complement(3947..4624)
                     /locus_tag="VGB68_20545"
     CDS             complement(3947..4624)
                     /locus_tag="VGB68_20545"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY0051699.1"
                     /translation="MLEAKKSRWFEEVFAVYNRNLLKRRFYSLDVSGLKFLKNQNHDA
                     PFLIYANHSSWWDGLVAFQISRQAESNNFIMMEEKQLKKLFLFRRLGAFSVVRENPRA
                     AAESLSYAANLLKENSNRALWIFPQGEILPNDKRPLNFYRGIARIIEKIGRVAAVPLA
                     IRYEFLGEFKPRIFVKIGAPQLISVDKSFDAKALTEQFEQRLTETLDALKTDVSAAAF
                     DDYEKIV"
     gene            complement(4713..>4997)
                     /locus_tag="VGB68_20550"
     CDS             complement(4713..>4997)
                     /locus_tag="VGB68_20550"
                     /inference="COORDINATES: protein motif:HMM:NF013038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HEY0051700.1"
                     /translation="IGYQDGYMRGFSNCSKAIVGGRFAGVAGRVSMDWTLIDVTDVPN
                     VKVGDEVVLIGEQNGIRIAAEELAAHCGTNSYEVTCGISRRVTRIYKNES"
BASE COUNT         1144 a         1334 c         1216 g         1303 t
ORIGIN      
        1 tcgaaaggga gcgccataaa ttaaaaccat taaagttatc gtccgaacaa actttaatgg
       61 ttttaattta tgagccggtt tcgcccgccg tttaaccgtc ggcggtgctt ttatccaaaa
      121 atatccggta agccgccgaa agcgcaatcg cgccgacaat cgcgcacagc aggttgagca
      181 gaaagctcgg cagcgtaaaa tcttccggcg agtttccggt tctaaagccg aagccgaaaa
      241 taaaacgccc gatgacgccg ccgacaatcg cgccgccgat gccgagcaga atcgtcgtca
      301 acatcccgcc cgtatgcctc gaagccatca aaaatctggc aatcaaaccg acaatcaagc
      361 cgaaaattat ccaggaaatt atcgtccaca atatttcgtt cgttccaaac ataatccttc
      421 tccggttctc ctttaaaagc ctttattttg taaccaacgg gaaaatgcct ttacccgatt
      481 gctcttttta agtgtctttt attttctacc tttttttcct gcttggcaat atctttcagt
      541 tcataaagct tcatcaaggc ttcgaccggc gaaagactgt cggtgtcgat ttccgataat
      601 ttttttcgta attcgtcgtc aagcgggctg gcgactcccg gctcgtcact ttcgacttta
      661 ccttcgtatt cttttaaaag ctcctcggcg cgtcgaatca ccggattcgg caaaccggcg
      721 agcctggcgg catagacgcc gtagcttttc agcgcgacgc cgcgcgagat tttgtattta
      781 aaggttaatt ctccgttgct ttcttcaatc tcgacgtgca gattttccag atgcggcaaa
      841 acgccttcca gctcagccag ctcgtgataa tgagtggcaa aaagcgtccg cgttttcaat
      901 ttcggatgat tgtggacgaa ttccagaacc gcgcgcgcca ccgccaagcc gtcaaaagtc
      961 gaagttccgc gtccgagctc gtcgagcagt atgagcgagc gcggcgtcgc gtgatgcagg
     1021 atttccgccg tttcgatcat ctcggtcata aacgtcgatt cgcccgcgcc gatacggtca
     1081 tacaagccga tgcgcgtaaa aatgcggtcg cacaagccga ccaccgcgtc ttccgccgga
     1141 acgaagctgc cgatttgcgc cagcagaacg attaaagcga tttgccgcag ataagtcgat
     1201 ttgccggatg cgttcggtcc cgtaatcagt gcgatattcg gcgcgccgtt gccgccgagc
     1261 gccgaatcgt tggcgacgaa ccggacgttt tcttcgcgca gaattttttc gacgacggcg
     1321 tgacgaccgt ttttaatgcg caaaagcgaa gtttcgttaa cggtcggacg cgaataattc
     1381 atctcgtcgg cggcgtcagc caagcctgta atcgcgtcca gataggcaat cgtcgaagcc
     1441 gccagcaaaa tctgcggtcg cgctttgccg atttccccgc aaacctgccg gaacagcgaa
     1501 tgttccagct cggctattct ttctttggcg tggacgacca gagattcgtg ttctttcagc
     1561 tccagcgtga tatatctttc cgcgggaata agcgtttgct tgcgcacgta attgctcgga
     1621 accaggtgca gattcgattt cgtgacctcg atgtaatagc cgaaaacctt attgtacccg
     1681 acgcgcaggc ttttgattcc ggtgcgctcg cgctcgcgtt tttccatctc ggctaaaaag
     1741 cctttgccgt cataaagcgt cgcgcgcaac ttgtccagct cttcggaaaa gccctcgcgg
     1801 ataattccga cctgttcgac gtgacgattc ggcagctctt cgctgattgc cgaatcgatc
     1861 aattttgccg tcgggctgca atccggcaga tgcgccagca gttgattgaa acgaaccgtg
     1921 tcgttttgca gaatctgttt gattttcgga attaattcca gactttttcc gaaggcgcga
     1981 agctcgtaag cggaaataaa attcgcgcga gcgcggctcg aaagtcgctc caaatccttc
     2041 atttccgaga aaatatacgc cagttcccgg cgctcgtgcg gattgtcctt aaaccacgcg
     2101 atatgctcct ggcgcttgta aatctccgcc gtatccagaa gcggctgccg cagccagcgc
     2161 cgcaaaagcc tgccgcccat tgcggttttc gtgcggtcga tgacggaaag caggctcgcg
     2221 tcagccgcgc tttcaaaaat ttccagactc cgcagcgtat gcgcgtcaat cagcataaaa
     2281 tcgcggctgt cgtagctggt caggcgcgtt aactgctccg ccgttccgct ctgcgattcg
     2341 ttcagatata aaatcagagc gcccgccgcg cttgttgcca gaggcgattt ttcgagtccg
     2401 aaaggctgca gggttttcgt gctgaaatga ttgaaaagcg tttttcgtgc cgtttcgagc
     2461 gagaaatatt cggcgtttaa tctggtaaca aaggtcggta aattttgatt gctgaaagtt
     2521 tcttccgcgt ccgccaaaat aatttcggcg ggagcgagcc gctgcaattc ggttatggcg
     2581 tcggaatttt cgatttccgt agcggcaaaa tctccggtcg ttacgtcggc ataagcgatt
     2641 ccggcgcgca gcccgtcgct gacgaaggcg cacagataat tattcgtttt gctttcgagc
     2701 aggctcggct caatcaccgt tccggcggta acgatgcgga cgacttcgcg ctgaatcagt
     2761 tttttgccgt cttttccttt gacgggagcg ctgctggtct gttcgcaaat cgccacgcga
     2821 tgcccgcgcg cgatgagcgt cgccagatga cgttcgagca caaaatgcgg cacgccagcc
     2881 agcggcacgc gctcgtcttt tcccatcgag cgtgatgtga gaacgatatc gagctctttc
     2941 gccagaagca cggcatcctc gtcaaaggct tcgtaaaaat cgccgagccg aaaaagcaaa
     3001 atcgcgtccc tgtgtttggc tttgatttcg agatactgcc ggcgaatcgg cgtttctgct
     3061 ttcataaaat aattttcgca cagagtgcac agcgcttaac ttagagtgtt taaattcaaa
     3121 ttacacgaat taattaaaat tgcaaagtct tcttcttaga gcttttctat attctttccg
     3181 ggtgttttcg gcggctaaaa ttatctttca agaaatttcg gcagaatctc ggcaatcgcc
     3241 cgcagattgc ggtgaatcct ttcgagctcg gcaaagctca gaatcgttaa aacatcggct
     3301 aattcgtgaa attcgcgttc cagcgattgt ctggcgcggt tggtgtcgcg ctccgaggcg
     3361 aggacgagaa aaggtttggt gcgctgcgcg tcgtgcgcgc gttttaattt ggcaaaagcc
     3421 gagtcgatat tgcctttgct ctgcacttcg aaaatgtgcg aaagcctttg cgcggagcgc
     3481 ttttcgcgcc agacgacgtc gtaatattca aactcggttt gggcgtcata acccaggctg
     3541 gcgccgattt cgacgagcat ttcctgaatc tctcggtgat ttaattcctt ttgttcgggt
     3601 ttggaaacct gaaagccttc gctctcgctg gcgacggtgc gttttccctt ttcggtcaga
     3661 ttccagaagc cgcgattgcg ctctaaaaga cctttctcgt caagcttttt tcccgctttt
     3721 tgaatcagct ttcgccagtt cgctttcgtc ccgtttctga tttcgcgaat ctccgtctcg
     3781 ctcaactgcg ggaaataagc cgtcagccgc tcgtataaaa aacgcacgtc ttccgcgccg
     3841 ccgatggcgt ataactcttg cagaaccggc atttcgattg acgaagactt aggaaacggc
     3901 atctcgtaaa ctcagattcc cgaaggcgag cggatttgtt ttatttttaa acgattttct
     3961 cgtaatcatc aaacgctgcc gccgaaacat ccgtttttaa agcgtccagc gtttcggtta
     4021 atctctgctc gaattgttcg gtcaaagctt tggcgtcgaa acttttatcg acgctaatca
     4081 attgcggcgc gccgatttta acaaaaatgc gcggtttaaa ttcgcccaga aattcatatc
     4141 gaatcgccag cggaacggcg gcgacccgac cgattttctc gattatgcgc gcgatgcctc
     4201 tgtaaaaatt cagcggtcgt ttgtcattcg gcaaaatctc gccctgcgga aaaatccaga
     4261 gcgctcggtt tgaattttct ttcagcagat ttgccgcgta gctcaggctt tcggcggctg
     4321 cgcgcggatt ttcgcggacg acggaaaatg cgccgagccg ccggaagaga aacagctttt
     4381 tcaattgttt ctcttccatc attataaaat tgtttgattc ggcttggcgc gaaatctgaa
     4441 aagcgaccag cccgtcccac cagctcgaat gattcgcgta gattaaaaaa ggcgcgtcgt
     4501 gattttgatt tttcaaaaat ttcaagccgg aaacatcgag cgaataaaag cggcgcttga
     4561 gcaaattgcg attgtatacg gcaaaaactt cttcaaacca acggcttttc ttcgcttcga
     4621 gcataagaga atcgtaaatc gtcagccgcg aatcgcaagc ggagaaaatg gaaaaatgaa
     4681 tctaaaattg aattttttaa acgattcgcc atttacgatt catttttata tatccgcgtc
     4741 acgcgcctgc tgattccgca ggtgacttca taagaattcg ttccgcaatg cgccgccagc
     4801 tcttccgccg cgattcttat tccgttttgt tcgccgatta aaactacctc gtcgccgacc
     4861 tttacattcg gcacgtccgt gacgtcaatc aaagtccagt ccatcgaaac gcgcccggcg
     4921 actccggcaa acctgcctcc gacgattgcc ttcgagcaat ttgaaaagcc gcgcatataa
     4981 ccgtcctggt agccgat
//
LOCUS       DASWNE010001076         2351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_349459, whole genome shotgun sequence.
ACCESSION   DASWNE010001076 DASWNE010000000
VERSION     DASWNE010001076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349459"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..>2351)
                     /locus_tag="VGB68_20555"
     CDS             complement(80..>2351)
                     /locus_tag="VGB68_20555"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEY0051701.1"
                     /translation="AFREINDTQGAGTLQHLESAVLQNNKSEEFAEKLQNFSVRLVLT
                     AHPTQFYPGSVLGIINDLTRAIKENDVAGINTFLQQLGRTPFFKKQKPTPFDEATSLI
                     WYLENVFYEAIGNIVSDLKTSFPGQPIETGELIKMGFWSGGDRDGNPFVTTRTTLKTA
                     AALRRAILQSYYRDVRKLKRRLTFPGVESVLAELETKLYDETFSGAENTDLNKQEILD
                     YLQNIRETLINRHNGLFVHLVNNLIDKVGIFGLHFASLDVRQEASVHGKILEDAAAKG
                     FLAEDYASLSDAQKIETLLNVKQAADAEIFEDELAKDTLQNVAAVKQIQRQNGSKGCE
                     RYIISQCGSALNIIEVYGLFLLGGWQATALSVDIVPLFETIADLKNASRVMRELYEIP
                     AYREHLARRSDTQTIMLGFSDGTKDGGYLMANWSIYKAKDELTKLSRERGVNVVFFDG
                     RGGPPARGGGKTHKFYSSMGENISNQAIELTIQGQTVSSNFGTIDAAQYNIEQLLHAG
                     IYNHLFSAKENTFSPAEETLMQSLADESFRAYEDLKNRPAFLDYLSHVSPLRFYVETN
                     IGSRPAKRGASGNKLSLKDLRAIPFVGAWSQLKQNVPGFYGVGAALQKSDKAGKLNEI
                     KALYRQNAFFKALIDNCEMAMQKSFFPLTGFLAEHPIYGDVQRNIYNEFELCRKYLAE
                     ISGKSALMSDYPVEQQSVATRERIVLPLTTIQQYALSKIREMEETETGGEQKNTFEKL
                     AMRCSFGIINAGRNSA"
BASE COUNT          498 a          614 c          604 g          635 t
ORIGIN      
        1 aaatcctgtt tatcctgttt taaatctatg tttatctgtg gttaaatttt ctttcttcgt
       61 gtctttgcgg tgactcgggt taagccgaat ttctgcccgc gttgatgatg ccgaaagagc
      121 agcgcatcgc cagtttttca aaagtatttt tctgctcgcc gccggtttcg gtctcttcca
      181 tttccctgat tttgctcagg gcgtattgct gaatcgtcgt cagcggcaaa acgattcgct
      241 cgcgcgtggc gaccgattgc tgctcgaccg gataatcgga catcagcgcg cttttgccgg
      301 aaatttccgc cagatatttc cggcaaagct cgaattcatt gtaaatattg cgctgcacgt
      361 cgccgtaaat cggatgctcg gcgagaaacc cggtgagcgg aaaaaaagat ttctgcatcg
      421 ccatctcgca attgtcaatc agggctttaa agaaagcatt ttgccggtac agcgctttga
      481 tttcattcaa ttttcccgct ttatcggatt tttgcagagc cgcgccgacg ccgtaaaagc
      541 cgggaacgtt ttgcttgagc tggctccagg cgccgacaaa cgggatcgcc cgcaaatctt
      601 ttaggcttag tttattaccg gacgcgccgc gttttgccgg acggctgccg atattggttt
      661 ccacgtaaaa acggagcggg ctgacgtgcg acagataatc cagaaacgcc ggtcgatttt
      721 tcaaatcttc ataggcgcga aagctctcgt ccgccagact ctgcatcagc gtttcctcgg
      781 cgggcgaaaa cgtattttct tttgccgaga aaagatgatt gtaaattccg gcgtgcagca
      841 actgttcgat attgtattgc gccgcgtcga tcgtgccgaa attcgagctg acggtttgtc
      901 cctgaatcgt caattcgatg gcttgattcg agatgttttc gcccatcgaa gaataaaatt
      961 tgtgcgtttt cccgccgccg cgcgccggcg gaccgccgcg accgtcaaag aaaacgacgt
     1021 tgacgccgcg ctcgcgcgaa agttttgtca gctcgtcttt cgccttgtag atgctccagt
     1081 ttgccatcag gtaaccgccg tctttcgtgc cgtcgctgaa gccgagcata atcgtctgcg
     1141 tgtcgctgcg gcgcgccaga tgctcgcgat aagccggaat ttcgtaaagc tcgcgcatta
     1201 cgcgggaagc gtttttcaaa tcggcaatcg tttcaaaaag cggcacgata tcgacgctca
     1261 aagcggttgc ctgccagccg ccgagcaaaa acagaccgta aacttcgata atgttcaaag
     1321 cgctcccgca ctggctgata atgtatcttt cgcagccttt cgagccgttt tgtcgctgga
     1381 tttgtttgac ggcggcgacg ttctgcaaag tgtctttcgc taattcgtct tcgaaaatct
     1441 cggcatcggc ggcttgtttg acgttcagaa gcgtttcgat tttttgcgcg tcggaaagcg
     1501 aggcgtaatc ttccgcgaga aatcctttgg cggcggcgtc ttccagaatt tttccgtgca
     1561 cgctcgcttc ctgccgcacg tcgagcgaag cgaaatgaag cccgaaaatt ccgactttat
     1621 caatcagatt attaaccaga tgcacgaaca aaccgttatg ccgattgatt aaagtctcgc
     1681 ggatattttg caggtaatcc agaatttcct gtttgttcaa atcggtgttt tccgcgccgg
     1741 aaaacgtttc gtcgtaaagc tttgtttcca gctccgccag aacgctctcg acgccgggaa
     1801 aagtcaatcg acgctttaat ttacgcacgt cgcgatagta gctctggaga atcgcgcggc
     1861 gcagagcggc ggcggtcttg agcgtcgtcc gcgtcgtcac aaacggattg ccgtcgcggt
     1921 cgccgcccga ccagaaaccc atttttatca gctcgccggt ctcgattggc tgccccggaa
     1981 atgaggtttt taaatccgag acgatgtttc cgattgcctc gtaaaagacg ttttcgagat
     2041 accagatgag gctcgtcgct tcgtcaaacg gcgtcggctt ttgctttttg aaaaacggcg
     2101 tgcgtcccaa ttgctgcaaa aaagtattga tgccggcaac gtcgttttcc ttaattgcgc
     2161 gcgtcaaatc gttgatgatg cccagaaccg agccgggata aaattgtgtc gggtgcgccg
     2221 ttaaaaccag cctgacgctg aaattttgca gcttttcggc gaattcctcg cttttgttgt
     2281 tttgcaggac tgccgattcc agatgctgga gcgttcccgc gccctgcgtg tcgtttattt
     2341 cgcgaaacgc g
//
LOCUS       DASWNE010001077         3198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_421215, whole genome shotgun sequence.
ACCESSION   DASWNE010001077 DASWNE010000000
VERSION     DASWNE010001077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421215"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..1793)
                     /locus_tag="VGB68_20560"
     CDS             complement(30..1793)
                     /locus_tag="VGB68_20560"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY0051702.1"
                     /translation="MLTLFHLFALFLLVLLGFYVFVANPRNRAHQTFAAFIAFLALWT
                     IKDLVFWDFHNLNTSAGWWAASSFIISLLMQCSLVVFAWVFPENARTPRKKAAVLFAP
                     GLVLIPAAAFGLLWRRVGFVDGRFEIELAPLAYAFVIYVYAVFAYGAWILLRKYRKYR
                     GTQKGQQVGAILWAVGVTGVLKTLANIFLPYFGSYEFLPISSLFVLPGVLIYAYAISN
                     FKLFSLQTALDQFRLFPITYKIALSIATVAIISFIAFQIPIVWWAFQDGMSFEAWRKY
                     LVFSIISALVPNLLLVLLIVRTISRPLQRITVAAVRVTEGEYGTEIDMRRSNDEIGLL
                     AESFNEMSRKMANDIEQLQKLNEQLIRTEKLAAMGTLAAGVAHEVNNPLASISSLIQM
                     LQKREHLDAETKEKLKLISTQITRITQVTRDMMDFARARPAAKSPIDVNAILQTSLRL
                     ASFDKAFQRLHLKTDLNENLPKVFADGDQLEQVFLNLLLNARDAMPEGGELSIKTFRG
                     DGSIAVRIADSGVGIEEKNLKKIFDPFFSTKPTGAGTGLGLAVCYGIVTAHNGKIEVE
                     SNGKGGTSFLVTLPADEAITN"
     gene            complement(1904..2644)
                     /locus_tag="VGB68_20565"
     CDS             complement(1904..2644)
                     /locus_tag="VGB68_20565"
                     /inference="COORDINATES: protein motif:HMM:NF022142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HEY0051703.1"
                     /translation="MKLKILFFLILFCSVSAASQTTPPESDFQFWNETQLAVPLVKSK
                     DKDGKDFERVTLFLTGTLRFGRNYAQLVDERIGLGFDFKINKYLTLTPSYLYRAAQPY
                     QNRNEFENRFRFAATVEKKWTKFSARDRNLIEYRARNSRADSVRYRNKLQFNFPVHKN
                     RKEIFAPFVSDEIFYDFDDRAWTRNELTLGVSRKFTPDFSADFFYLNQRNRGNVLKNV
                     NAFGINLKFKFDWRNKESDTETPSPDRR"
     gene            complement(2704..>3198)
                     /locus_tag="VGB68_20570"
     CDS             complement(2704..>3198)
                     /locus_tag="VGB68_20570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051704.1"
                     /translation="LGTEKAIAYFGAMLGLFPPFALFSRFLFENFKTPTASDDFWIIP
                     LLLFVNFVCMVVGYFSGKMVGKIVVELEKHSWSSMLLTMPFIGVLWGIITGAAGGIFI
                     FVIGAFAGAAVAAAVGAVALPAFTIFHRWLKRGDSIEEKHFFPIALGISCIITALILG
                     LKIS"
BASE COUNT          865 a          793 c          802 g          738 t
ORIGIN      
        1 atattctttt aattcgtaat tcgtaatttt taattcgtaa ttgcttcgtc tgcgggcaaa
       61 gtcaccaaaa aggacgttcc gccttttccg ttcgattcga cctcgatttt tccgttgtgc
      121 gccgtgacga ttccgtaaca gacggctaag cccaaaccgg ttcccgcgcc ggtcggcttg
      181 gtcgaaaaaa acgggtcgaa aatctttttc agattttttt cctcgatgcc gacgccgctg
      241 tcggcgattc ggacggcaat cgagccgtcg ccgcgaaagg tttttatcga aagctcgccg
      301 ccttcgggca tcgcgtcgcg cgcgttgagc agaagattca aaaaaacctg ttccagctgg
      361 tcgccgtcgg cgaaaacttt cggcagattt tcgtttaaat cggtttttag atgcaggcgc
      421 tgaaaggctt tatcgaagct cgccaggcgc aggctggtct gcagaatcgc attgacatcg
      481 attgggcttt ttgccgccgg gcgcgcgcgc gcaaaatcca tcatatcgcg cgtgacctgc
      541 gtgatgcgcg tgatttgcgt cgaaatcagc tttaattttt cctttgtttc cgcatccaga
      601 tgttcgcgtt tttgcagcat ttgaatcaag gatgaaatcg aagccagcgg attattaacc
      661 tcgtgcgcga cgcccgccgc gagcgtgccc atcgccgcca gcttttcggt gcgaatcaac
      721 tgctcgttga gtttctgcaa ttgctcgatg tcgttcgcca ttttgcggct catctcgtta
      781 aaagattccg ccagcagacc gatttcgtcg ttgctccggc gcatatctat ctccgtcccg
      841 tattcgcctt cggtgacgcg caccgccgcc accgttatgc gctgcagcgg gcgcgaaatc
      901 gtgcgaacaa tcaaaagaac gagaagcaga ttcggcacga gcgcggaaat aatgctgaaa
      961 accagatatt ttcgccacgc ttcaaagctc attccgtcct gaaacgccca ccagacgatc
     1021 gggatttgaa aggcgataaa actgatgatg gcgacggtcg cgatgctgag cgcgattttg
     1081 tatgtaatcg ggaaaagccg aaactggtcg agcgcggttt gcagcgagaa aagcttgaaa
     1141 ttggaaatcg cgtaagcgta aatcaagacg ccgggcagca cgaacagcga cgaaatcggc
     1201 agaaattcgt aactgccgaa atacggcaga aagatattcg ccagcgtttt taaaacgccc
     1261 gtcacgccca ccgcccagag aatcgcgccg acctgctgac ctttctgcgt gccgcgatat
     1321 ttccggtatt tgcgcaaaag aatccaggcg ccgtaggcga aaaccgcgta gacgtaaatc
     1381 acaaaagcgt aagccagcgg cgcgagctca atctcgaatc tgccgtccac aaagccgacc
     1441 ctgcgccaga gcagtccgaa tgccgccgcc ggaatcaaaa ccaaaccggg cgcaaataaa
     1501 acggcggctt tttttcgcgg cgtgcgcgcg ttttccggaa atacccacgc aaagacgacg
     1561 agcgagcact gcatcagcag agagattata aagctcgaag ccgcccacca gccggcggac
     1621 gtgttcaggt tgtgaaaatc ccagaaaact aaatctttta ttgtccagag cgcgagaaaa
     1681 gcgataaaag cggcgaaggt ttgatgcgcg cggttgcgcg ggttggcgac aaaaacgtaa
     1741 aagccgagca aaaccagcaa aaaaagcgcg aacagatgaa acagcgtcag catcagctag
     1801 attttaacgc aaagccgccg agaggcaagc gtttaaggca aaagtaaaaa gtaaaaaata
     1861 aaaagttaaa atgaaacgcc gattattttt atgagcgaat tatttatctt cggtcgggcg
     1921 acggcgtttc cgtatcggat tctttgtttc tccaatcgaa tttgaatttc agattaattc
     1981 cgaacgcgtt tacatttttt aaaacattgc cgcgatttcg ctggttgaga taaaagaaat
     2041 cggcggaaaa atcgggagtg aatttacggc tgacgccgag cgtcagctcg ttgcgcgtcc
     2101 aggctcggtc gtcgaaatcg taaaatattt cgtccgagac aaagggcgcg aaaatttctt
     2161 tccggttctt gtgaacggga aaattaaatt gcagcttgtt gcgatagcgc accgaatcgg
     2221 cgcgcgagtt tctcgctcga tactcgatta aattccggtc gcgggcggaa aatttcgtcc
     2281 acttcttttc cacagtagcg gcaaatctga agcggttttc aaattcattt ctgttttgat
     2341 aaggctgtgc ggcgcggtaa agatagctgg gcgttaaggt caggtattta ttaattttaa
     2401 aatcaaagcc caaaccgatt ctttcatcga ccaattgcgc atagtttctg ccgaagcgca
     2461 gagttccggt cagaaaaagc gtgactcttt cgaaatcttt tccgtctttg tccttggatt
     2521 tgaccagcgg aacggcaagc tgcgtttcgt tccagaattg aaaatccgat tcgggcggcg
     2581 ttgtttgcga agcggcggaa accgaacaaa ataatattaa gaaaaacaaa attttaagtt
     2641 tcatcgagaa gcgtctgcgt tacaaaaata ttaaatttat gttacagaca aaataaatgc
     2701 ttcctatgaa attttcaagc cgaggataag cgccgtgata atgcagctga tgccgagcgc
     2761 aatcgggaaa aagtgttttt cttcaatcga atcgcctctt ttcagccagc gatgaaaaat
     2821 cgtaaacgcg ggcagcgcga cggcgccgac cgccgccgcg acggcagccc cggcaaacgc
     2881 gccgataaca aaaatgaata tgccgccagc cgcgcccgtg atgatgcccc ataaaacgcc
     2941 gatgaaaggc atcgtcagaa gcatcgacga ccaggaatgt ttttccagct cgacgactat
     3001 tttgccgacc atttttcccg aaaaatagcc gacgaccata cagacgaaat tgacaaaaag
     3061 caatagcgga ataatccaga aatcgtcaga agccgtcggg gttttaaaat tctcgaacaa
     3121 aaagcggcta aacaatgcaa acggcggaaa aagtccgagc atcgcgccga aataggcaat
     3181 cgctttttcg gtgccgag
//
LOCUS       DASWNE010001078         2704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_421504, whole genome shotgun sequence.
ACCESSION   DASWNE010001078 DASWNE010000000
VERSION     DASWNE010001078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421504"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1333)
                     /locus_tag="VGB68_20575"
     CDS             complement(<1..1333)
                     /locus_tag="VGB68_20575"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEY0051705.1"
                     /translation="MDDLLAARSQMAMSLAFHIIFAAVGIAMPLLMVIAEWRWLRTKD
                     EVYLTLTKRWAKGTAILFAVGAVSGTVLSFELGLLFPGFMGYAGSIIGMPFSLEGFAF
                     FTEAIFLGIYLYGWNRISARAHLLAGVIVAASGALSGIFVVIANAWMNAPTGFRLVDG
                     KMVDIDPIAAMLNPAAFSQTLHMTLAAYAATGFVVAGIHAFMLLRDRNNRFHRHALSI
                     ALTVGGITAILQPLSGDILAKAVAKNQPAKLAAFEGQFETETGAPLRIGGIPDEESGV
                     TRYAIEIPYALSILAHGDPNATVKGLNEFPKDERPPVAIVHFAFQIMVGCGMLMAAIA
                     VWGAWRWRRNRRREAGKWLDSKWFLRALILAAPLGFIAIETGWVVTEVGRQPWIIYGV
                     MRTSEAVTPMPGLVVPFITFTILYIFLAVITAWLLLRQVVASPSEPSAAAGG"
     gene            complement(1478..1696)
                     /locus_tag="VGB68_20580"
     CDS             complement(1478..1696)
                     /locus_tag="VGB68_20580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051706.1"
                     /translation="MATLLDELNQLISALEENDIEYAVCGGLALTIHGFPRATFGIDI
                     LIQSESLEKAYEIAAKKGYDIRGLDISF"
     gene            complement(1745..2269)
                     /locus_tag="VGB68_20585"
     CDS             complement(1745..2269)
                     /locus_tag="VGB68_20585"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF009763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000334 - 3-hydroxyanthranilate
                     3,4-dioxygenase activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HEY0051707.1"
                     /translation="MSIRRPFNFKQWIEEHRHLLKPPVGNQCAYDAEDFIVMVVGGPN
                     ARKDYHYDEGEEFFYQLEGDITVKIQEDGRAVDVQVREGEIFLLPPRVPHNPVRGANT
                     VGMVIERKRRAGEKDGLLWFCENCNEKLYEEYFELEDITMQFQGVFKRFYENEDLRTC
                     KNCGAVMQPPAVAS"
     gene            complement(2408..>2704)
                     /locus_tag="VGB68_20590"
     CDS             complement(2408..>2704)
                     /locus_tag="VGB68_20590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051708.1"
                     /translation="RAIFVKAPFGSKNAYAAPETYLWQVVLSEVPGNYKSNDVFYQLR
                     EQVCNCKRIRLEGFSLTKCFIAGTGHPNIAGARKYADAIEREFGEVLPLTGWRN"
BASE COUNT          667 a          761 c          643 g          633 t
ORIGIN      
        1 aaccgcccgc cgcggcagac ggttccgacg gactcgcgac gacctgccgc agcaataacc
       61 aagccgtgat aacagcgaga aaaatatata gaatcgtgaa agtaatgaac ggcacgacca
      121 gtccgggcat cggcgtcacc gcttccgacg ttcgcatcac gccgtagata atccagggct
      181 ggcgtccgac ttccgtcacc acccatcctg tttcaatcgc gataaaaccg agcggcgcgg
      241 ctaaaatcag cgcccgcaaa aaccattttg aatcaagcca tttccccgct tcccggcggc
      301 gattgcgccg ccagcgccac gcgccccaaa cggcgattgc cgccatcagc attccgcagc
      361 cgaccataat ctgaaaggcg aaatggacga tagcgacggg cggtcgctcg tctttaggaa
      421 attcgtttaa gcctttcacg gtcgcgttcg ggtcgccgtg cgcgagaatg ctcaaagcat
      481 acggaatctc gatggcgtag cgcgtcacgc cggattcctc gtcgggaata ccgccgatgc
      541 gaagcggcgc gcccgtttcg gtttcaaatt gtccttcgaa agcggcaagc ttggcgggtt
      601 gattcttcgc cacggcttta gcgagaatgt cgccgctcaa aggctgtaaa atcgccgtta
      661 ttccgccgac cgtcaaagca atcgagagcg cgtgccggtg aaaacggttg tttctgtcgc
      721 gcagaagcat aaaagcgtgt attccggcga caacaaaacc ggtcgcggca taagcggcaa
      781 gcgtcatatg cagcgtctgc gaaaacgccg ccggattcag catcgccgca atcgggtcga
      841 tatcgaccat ctttccgtca accagacgaa aacccgtcgg cgcgttcatc cacgcgtttg
      901 cgataacgac gaaaattccc gaaagcgcgc cgcttgccgc cacaatcacg cccgccagca
      961 aatgcgcccg cgccgagatg cgattccagc cgtaaaggta aatcccgaga aaaatcgctt
     1021 cggtgaaaaa cgcgaagcct tcgagcgaaa acggcatccc gataatcgac cccgcgtagc
     1081 ccataaagcc cggaaacagc aaaccgagct caaaagacag cactgtgccc gaaaccgcgc
     1141 cgaccgcaaa aagtatcgcc gttcccttcg cccagcgttt cgtcagcgtg agataaactt
     1201 cgtctttagt gcgcagccag cgccattccg caatcaccat caaaagcggc attgcgattc
     1261 cgaccgcggc gaaaatgatg tgaaacgcga gcgacatcgc catctgcgag cgcgccgcga
     1321 gcaggtcatc catcaacgat tgtctccggt tttatgaaag tatagttatc ttttaagttc
     1381 atcagcggag attttattaa atatttttta atttccggca gcttaaacga cgatggcgga
     1441 ttttccggtt tattttttct ttcttcaaca ctctttccta aaacgaaata tcgagtccgc
     1501 gaatatcata accctttttc gcagctattt cgtaggcttt ttcaagcgat tcggattgaa
     1561 tcaaaatatc aataccgaag gtcgcccgcg gaaagccgtg aatcgttaaa gccagaccgc
     1621 cgcaaaccgc gtattcgatg tcgttttctt cgagcgcgga aatcaactga tttaattcat
     1681 ctaaaagcgt cgccattttg gtttatttac gaattgccga atattattat tttctcgtcg
     1741 ataatcagct cgcgaccgcc ggcggctgca ttaccgcgcc gcagtttttg caggttcgca
     1801 aatcctcgtt ttcgtaaaag cgcttgaaaa cgccctgaaa ctgcatcgtg atgtcttcca
     1861 gctcaaaata ttcttcgtat aatttctcgt tacagttttc gcaaaaccat aaaagcccgt
     1921 ctttttcgcc ggcgcgccgt ttgcgttcaa tcaccattcc cacggtattt gcgccgcgca
     1981 cagggttgtg cggcacgcgc ggcggcagca gaaaaatctc gccttcgcgt acctgaacgt
     2041 cgaccgcgcg cccgtcttcc tgaatcttga cggtaatatc gccttcgagc tggtagaaaa
     2101 attcctcgcc ttcgtcgtaa tgataatcct tgcgcgcatt cggtccgccg acgaccatca
     2161 cgataaaatc ttcggcgtcg taagcgcatt gattgccgac gggcggtttc agcaggtgcc
     2221 gatgttcttc aatccattgt ttgaaattaa aaggtcttct gatagacata aattttaacc
     2281 tcgattttcg ctgtgcattt cctgtttttt acgaaatcgc aaaataaata aattgtaatc
     2341 ccaagcagcc ttaaaagaga atattccgaa gcgaggcggt taatttttcc gctttttcgg
     2401 aaagctttta attacgccag ccggttaaag gcaaaacttc gccgaattcc cgctcgatag
     2461 cgtcggcata ttttcttgcg ccggcaatat tcggatgtcc ggttccggcg ataaaacatt
     2521 ttgtcaaact aaaaccttcg agccgtatgc gcttgcagtt gcaaacctgc tcgcgcagtt
     2581 gatagaaaac gtcgttcgat ttgtaattgc ccggaacctc gctcagaacc acttgccaca
     2641 gataggtttc cggcgcggcg taggcgtttt tcgagccgaa aggagctttg acgaaaatcg
     2701 cccg
//
LOCUS       DASWNE010001079         2111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_421689, whole genome shotgun sequence.
ACCESSION   DASWNE010001079 DASWNE010000000
VERSION     DASWNE010001079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421689"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /gene="xseA"
                     /locus_tag="VGB68_20595"
     CDS             complement(<1..691)
                     /gene="xseA"
                     /locus_tag="VGB68_20595"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEY0051709.1"
                     /translation="MKSSLLQSLFDDEERRPLSVTELNEQVKGALERSFASVWIEAEI
                     VNFVAANSGHWYFSLHDGFSQLKAACYIRNNLRIRFQPFDGLQVRVRGKLSVYQPKGE
                     YQILVESLEPVGEGALKVAFEQIKAKLSKEGLFDEDLKRDLPFFPRRVGVVTSPNGAA
                     FHDILNVLSRRTRTVHVTLIPTRVQGETAGEEITAAIELANEYNKTAREKIDVLIVGR
                     GGGSSEDLWAFN"
     gene            complement(691..1530)
                     /locus_tag="VGB68_20600"
     CDS             complement(691..1530)
                     /locus_tag="VGB68_20600"
                     /inference="COORDINATES: protein motif:HMM:NF014177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEY0051710.1"
                     /translation="MIRSPLRYPGGKSRAVEKITSLIPPFQEFREPFVGGGSIFIHLR
                     QKFPGKNFWINDIYTDLAVFWQEIQLNCAPVIEQIWKWRSDFQNGREFHKFLRENKSN
                     FNRVEQAAAFFVFNRITFSGTTEAGGFSIQAFEKRFTDSSIERLEKTPEILRDVKITN
                     LDYDEVIKASGENVFLFLDPPYFSATNSALYGKNGNLHKTFDHERLAETLRRCPHRWL
                     LTYDDSPYVRELFSFANVSGWNLMYGMRNVAENSSQKGNELFISNYEPETERRKQIAF
                     NFA"
     gene            complement(1533..1706)
                     /locus_tag="VGB68_20605"
     CDS             complement(1533..1706)
                     /locus_tag="VGB68_20605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051711.1"
                     /translation="MEITAEIIAGIKYKQHLKCDLTIASLENFDVNSAKSSMKILFAE
                     ARENDFSVILEKG"
     gene            complement(1707..>2111)
                     /locus_tag="VGB68_20610"
     CDS             complement(1707..>2111)
                     /locus_tag="VGB68_20610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051712.1"
                     /translation="KVTITNALFGKYFFTVDETKIEPRVVYLIEAKHSQRALLPSKND
                     IKDGLIKMMLYTNLSKVKVGARAFDYKVMIRLTSSRLKGSITSDAKDEESAKFFSDNS
                     IDAANREFLKKLFQEARENKFTIILEHAETAK"
BASE COUNT          515 a          524 c          496 g          576 t
ORIGIN      
        1 cgttaaaagc ccacaaatct tccgacgagc cgccgccgcg cccgacaatc aaaacgtcga
       61 ttttttcgcg cgccgttttg ttatattcat tggctaactc gatagcggcg gtgatttctt
      121 cgcccgccgt ttcgccctgc actctggtcg gaattaaagt cacgtgaacg gtgcgcgttc
      181 ttctggacaa aacgttcaga atatcgtgaa aagccgcgcc gttcggactg gtgacgacgc
      241 cgactcttct cgggaaaaac ggtaaatcgc gcttcaaatc ctcgtcgaat aaaccttctt
      301 tcgacagctt cgccttgatt tgctcaaaag cgactttcag cgcgccttcg ccgaccggct
      361 ccagcgattc gaccagaatc tgatattcgc ccttcggctg gtaaacgctc aattttccgc
      421 gcacgcgcac ctgaagaccg tcaaacggct gaaagcgaat ccgcagatta ttgcgaatgt
      481 aacaggcggc tttgagctgc gaaaaaccgt cgtgcagact gaaataccag tgaccggaat
      541 tggcggcgac gaaattgacg atttcggctt caatccagac ggacgcaaac gagcgctcaa
      601 gcgcgccctt aacctgctcg ttcagctccg tgacgctcaa aggtcggcgc tcctcgtcgt
      661 cgaaaagaga ttgtaaaagc gaagatttca tcaggcaaaa ttaaatgcga tttgttttcg
      721 tctctccgtt tccggctcgt aattcgagat aaaaagctcg tttcccttct gcgaagaatt
      781 ttcggctacg tttcgcattc catacatcag attccagccc gatacattgg caaacgaaaa
      841 aagctcgcgc acgtaaggcg aatcgtcgta ggtcaaaagc caccggtgag ggcaacggcg
      901 taaagtttcg gccaaccgct cgtggtcgaa agtcttgtgg agatttccgt ttttaccgta
      961 aagagccgaa ttcgtcgcgg aaaaatacgg cgggtcgaga aacaaaaaaa cattttcgcc
     1021 gctcgcttta atcacttcgt cgtaatcgag attcgtgatt ttcacatctc gcaaaatttc
     1081 aggcgttttt tccaatcgct cgatgctcga atcggtaaaa cgcttttcaa acgcctgaat
     1141 cgaaaatccg ccggcttccg tcgtgccgga aaaagtgatg cgattaaaaa caaaaaatgc
     1201 ggcggcttgt tcgacgcggt taaaattcga tttgttttcg cgcagaaatt tgtgaaactc
     1261 cctgccgttt tgaaaatcgc ttctccattt ccaaatctgc tcgataaccg gtgcgcaatt
     1321 aagttgaatt tcctgccaaa agacggctaa atccgtgtaa atatcgttta tccaaaaatt
     1381 tttaccggga aatttctgtc tgagatgaat aaatatcgag ccgccgccga caaacggctc
     1441 gcggaattcc tgaaaaggcg gaatcagact cgtaattttc tcgaccgcgc ggcttttccc
     1501 gcccgggtag cgtaaagggc tgcgaatcat atttaacctt tttccagaat aaccgaaaaa
     1561 tcgttttccc gcgcttcggc aaataaaatt ttcattgagg attttgccga attaacgtcg
     1621 aaattctcca atgaggcaat tgttaaatcg catttcagat gctgtttgta tttgattccg
     1681 gcgatgatct ccgctgtgat ttccatttat ttcgccgttt cggcgtgttc cagaattatc
     1741 gtgaatttat tttcgcgcgc ttcctgaaaa agctttttga gaaattcgcg gtttgcggcg
     1801 tcgatgctgt tgtccgagaa gaatttcgcc gattcttcgt ctttggcgtc tgaagtgatc
     1861 gagcctttca gacggctcga cgtcaggcga atcatcactt tgtaatcgaa ggctcgcgcg
     1921 ccgactttta ctttgctcag gtttgtgtag agcatcattt ttatcaagcc gtctttgatg
     1981 tcgtttttgc tcggcagcag agcgcgctgg ctgtgcttgg cttcgattag atagacgacg
     2041 cgcggttcga ttttcgtttc gtcgacggtg aaaaaatatt ttccgaacaa ggcgttggtg
     2101 atggtgactt t
//
LOCUS       DASWNE010001080         2916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_634698, whole genome shotgun sequence.
ACCESSION   DASWNE010001080 DASWNE010000000
VERSION     DASWNE010001080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634698"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="VGB68_20615"
     CDS             <1..788
                     /locus_tag="VGB68_20615"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY0051713.1"
                     /translation="VPSDNQSIHETNVPTMPACFLITMRDISVIKENEKLRSDMTSLM
                     SHELRTPITSIQGFAELLLADETIHEEAREFLTIIAGESQRLSKMLSTFLSVSNLEQS
                     DKREFFKTPVKLDTVVREVVDDLQETAKRKRIRLVEQSNAHLPPIAADKSLISRAVSH
                     LIDNAIKYSPERTSVIISTILESEFLRVVVEDRGYGIPKIEQEKIWQKFYRVARDGQD
                     KEEESTGLGLSLVKEIVEQHGGEVSVESEVGQGSKFSLKLPRL"
     gene            complement(1015..1794)
                     /locus_tag="VGB68_20620"
     CDS             complement(1015..1794)
                     /locus_tag="VGB68_20620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEY0051714.1"
                     /translation="MKDLDFPKNQEQRVRVVSLLPNVCNHESAIEKIAGLVQGGDGGY
                     VCFSTVHMVMESHDNPEYAAKVNGADFIITDGMPLVWMQKLQGRRDAARVRANDLMIL
                     LCEYAAKNNLSVGFYGGKQEVIDAIKERARKELPRLQIAYAFSPPFRQLTGEEDAAVT
                     AEINRAQPDILFMGLGCPKQENWMAAHRDKVKAVMLGVGASFDFYAGNVKESPEWLGK
                     LGLEWLYRLTQEPKRLWRRYLILNPRFMWLATLQLLRARKD"
     gene            complement(1795..>2916)
                     /locus_tag="VGB68_20625"
     CDS             complement(1795..>2916)
                     /locus_tag="VGB68_20625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY0051715.1"
                     /translation="NRLGYPFWFEMTVKKPIKVLITAPSLDERQNVSGISTVARLIIE
                     RGTFEYRHFESGRRDGERADAKWIWKQIVSVPRFRRAARGVDIVHFNTNFNPLAILRD
                     AAYASAARLSGIPVLMHVHGGRYLAEEFQSVWLKKIAGKMLRAADAVIVLSELEKKIV
                     ENRWQNLSVRVLENAVAVDEAIAARRKNDEKILIFFGRLHESKGLNEIIEACRILRNE
                     GFEFRFEAFGAGEMKEKFVSEMTEVLSEKFQYGGVLSGAEKWKELAKADFFLLPSRYG
                     EGLPMAMLEAMAAGCVVIVSEMASTGAVIVDGANGFLVAPRDANQLTEKLRLLLSGKI
                     DLENLRKNARATIKAKFDLGDYIKRLENIYAEILSAKSV"
BASE COUNT          706 a          825 c          683 g          702 t
ORIGIN      
        1 aggtgccttc cgacaatcaa tccatccacg aaaccaacgt gccgacgatg cccgcctgtt
       61 ttctgattac gatgcgcgat atttccgtca tcaaggaaaa cgaaaagctg cgcagcgata
      121 tgacgagcct gatgtcgcac gagctgcgca cgccgattac cagcattcag ggctttgccg
      181 agctgctgct cgccgacgag acaatccacg aggaagcgcg cgaattttta accatcatcg
      241 cgggcgaatc gcaaaggctt tccaagatgc tttcgacttt tctttcggtg tccaacctcg
      301 aacaatccga caaacgggaa tttttcaaaa cgcccgtcaa gctcgacacg gtcgtccgcg
      361 aggtggtcga tgatttacag gaaaccgcca agcgcaagcg aatccggctg gtcgaacaat
      421 cgaacgcgca tctgccgccg attgccgccg acaagagctt aatctcgcgc gccgtttcgc
      481 atttgattga caacgccatt aaatacagcc cggaaagaac ttccgtaata atctcgacga
      541 ttctggaatc ggaatttctg cgcgtcgtcg tcgaagaccg cggctacggc atcccgaaaa
      601 tagagcagga aaaaatctgg cagaaatttt atcgcgtcgc ccgcgacggg caggacaagg
      661 aagaagaatc gaccggcttg ggactatcgc tggtcaagga aatcgtcgaa cagcacggcg
      721 gcgaggtctc ggtcgaatcg gaagtcgggc aaggctcgaa attcagcttg aaactgccca
      781 gactataaga aaattcagcc gcgaaaggac aagaaaaaac gcgaaagaaa tcagccgcag
      841 agatttttga gaaaatgaaa ccacggattt tcacggataa acatagataa gaaatagata
      901 ttatcccgat aatcttgttt tcttatccga gttcattcgt gaaaatccgt ggttaatttc
      961 tctctttttc tctcgcgtcc tctgtggctg aattttcttt cggttttttc gcggtcaatc
     1021 cttcctcgct ctcaaaagct gtagcgtcgc cagccacata aaacgcggat tcaaaattaa
     1081 atatctgcgc cagagtcgtt tcggttcctg ggtcagccgg tagagccatt cgagccctaa
     1141 ttttccgagc cattccggcg attctttgac gtttccggcg taaaaatcaa acgacgcgcc
     1201 gacgccgagc atcacggctt ttactttgtc cctgtgcgcc gccatccagt tttcctgttt
     1261 cgggcagccc aagcccataa ataaaatgtc cggctgagcg cgattgattt ccgccgtcac
     1321 cgccgcgtct tcttcaccgg ttaattgtcg aaacggcggc gaaaaagcgt aagcgatttg
     1381 taaacgggga agctcttttc gcgcgcgttc tttaatggcg tcgatgactt cctgttttcc
     1441 gccgtagaaa ccgacgctca gattattttt cgcggcgtat tcgcacagca gaatcatcaa
     1501 gtcgtttgca cgcacgcgcg ccgcatcgcg cctaccctgc aatttctgca tccagaccag
     1561 cggcatcccg tcggtgatta taaaatccgc gccgttcact ttcgccgcgt attccggatt
     1621 gtcgtgcgat tccatcacca tatgaacggt ggaaaaacag acgtatccgc cgtcgccgcc
     1681 ctggacgagc cccgcgattt tttcaatcgc cgattcgtga ttgcagacat tcggcaataa
     1741 actgacgacg cgaacgcgct gctcctgatt tttcggaaaa tccaagtctt tcattcaaac
     1801 tgatttcgcg gataaaattt ccgcgtaaat attttccagt cgtttgatgt aatcgcccaa
     1861 atcgaatttc gctttaatcg tcgcccgcgc gttttttcgc aggttttcca aatcaatttt
     1921 accggaaagc aaaagcctga gcttttccgt taattgattt gcgtcgcgcg gcgcgaccag
     1981 aaaaccgttt gcgccgtcca caatcaccgc gccggtcgaa gccatttccg aaacaatcac
     2041 gacgcagccg gccgccatcg cttcgagcat cgccatcggc aagccttcgc cgtagcgcga
     2101 aggcagcaaa aaaaagtccg cttttgccag ttccttccat ttttccgcgc cggagagaac
     2161 gccgccgtat tgaaattttt cgctcaaaac ctctgtcatt tccgaaacga atttttcctt
     2221 catctcgccc gcgccgaaag cctcgaagcg aaactcaaag ccttcgtttc ttaaaatgcg
     2281 gcaggcttcg ataatttcat tcaagccttt cgattcgtgc agtcgtccga aaaaaatcaa
     2341 aatcttctcg tcgtttttcc gccgcgcggc aatcgcttcg tcaaccgcga cggcgttttc
     2401 cagcacgcgg acgctcagat tttgccagcg attttcgaca atcttttttt caagctcgct
     2461 caaaacgatt accgcgtccg ccgcgcgcag cattttcccg gcgatttttt tcagccaaac
     2521 gctttgaaat tcttccgcca gataacgccc gccgtgaacg tgcatcaaaa caggaatgcc
     2581 cgaaagacgc gccgccgacg cgtaagcggc atcgcgaagg atggcgagcg gattaaaatt
     2641 cgtgttgaaa tgaacaatgt cgacgccgcg cgccgcgcgc cggaaacgcg gcacggaaac
     2701 gatttgcttc caaatccatt tcgcatcggc tcgttcgccg tcgcgcctgc ccgattcaaa
     2761 atgccggtat tcgaaagttc cgcgctcgat aatcaggcgc gcgaccgtcg agattccgct
     2821 cacgttttgt ctttcgtcga ggctcggggc ggttatcaga acttttatcg gcttttttac
     2881 ggtcatttca aaccaaaaag gataacccag ccgatt
//
LOCUS       DASWNE010001081         3363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_350398, whole genome shotgun sequence.
ACCESSION   DASWNE010001081 DASWNE010000000
VERSION     DASWNE010001081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350398"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2190)
                     /locus_tag="VGB68_20630"
     CDS             complement(<1..2190)
                     /locus_tag="VGB68_20630"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY0051716.1"
                     /translation="MLAIIALGALCLAIALWTFPPEKLSLQFVFLSLLTIAFGSRISI
                     QIPRFKSHIAVSDTFIFLAILLFGGEAAILLSAVEAFFSSWRFCKKKISVFFNAALLA
                     CSTTFVVSLLRSFEIEGATVIHEKTHSNLIISLSVMALVQFIFNSGLASIYGALKSEK
                     PWWETWKTHYLWTSITYFVGAIAAGILAELVFVIGFGVIIAAAPVIVIVYLTYRMYLK
                     NVEMSLSQAEQAEQHALILEEQSRALAESEERFRSAFTYAPIGIGIVSPDGRWLKINK
                     ALSEILGYTEEEFLATEFQTMIYEDDLGDTLVKLHELTTGKVPACQLEQRYLNKRGEI
                     VWVSWSVSATSDHKAERPNLIFQIQDITVKKLTEEQLQYKATHDALTGLPNRAYFMAR
                     LEQALEKVRDNPTYKVSVLFIDLDRFKIVNDSLGHQIGDELLIGIAERLRECLRPSDM
                     VARLGGDEFTILVEGKYDPKEVVRIAERIREKFLSPFNLSGHEIFSSASIGILHASDK
                     YTKPEDVMRDADIAMYQAKRAGKSRHEIFNQHMRDAVKETLQIETDLRHAIEKQEFDV
                     YYQPIYSFETDKIEGFEALARWEHKTLGMIPPSRFIPLAEEIGVIDALGEQILRRACK
                     EGCLLQQSALEADSFVLSVNISCKQFAQPKFAQNIKQILEETNFSPRNLKLEITESVF
                     LEHTETAILILQQLCEIGIEINIDDFGTGYSNLHYLMQFPITTLKIDR"
     gene            complement(2406..3314)
                     /locus_tag="VGB68_20635"
     CDS             complement(2406..3314)
                     /locus_tag="VGB68_20635"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY0051717.1"
                     /translation="MSYQIVNRHTVQSLPAARIAVTLAPDMAQICELKDTDTAEALAF
                     LAMRPVHTIVMTSFINDNGLDSSLNRGRFYGFRDGDGKLEGVALIGHTTLVEARSEES
                     LKAFAFSGGESETPIHIIMSDGNSAELFWQYYSGGARQPRLICNELLFEISFPVMVGE
                     TTEDLRLAAEDELLEVAEAHAEVALMESGINPLEKDREGFLQRTLRRIRQERTFVVFE
                     NGKLLFKADIVAETVDVIYLEGIYVAPERRGAGAGSKYLSQLSRLLLNRVKHVCLLSN
                     VDFKEAHTAYLKAGFKSKDSCTTIFV"
BASE COUNT          819 a          821 c          872 g          851 t
ORIGIN      
        1 gcggtcgatt ttcagagtgg taatcgggaa ttgcatcaaa taatgcagat tcgaatagcc
       61 tgtgccgaag tcgtcgatat tgatttcaat gccgatttcg cacaactgct gcaaaatcag
      121 aatcgccgtt tcggtatgtt ccagaaaaac cgattcggta atttccagtt tcagatttcg
      181 cgggctgaaa ttcgtctctt cgagaatttg tttgatgttt tgggcaaatt tcggctgggc
      241 aaactgcttg caggaaatat taacgctcaa cacaaacgag tccgcttcga gcgcggattg
      301 ctggagcaga cagccttctt tgcaggcgcg gcgcaggatt tgctcgccga gcgcgtcaat
      361 cacgccgatt tcttccgcta gcggaataaa cctgctcggc ggaatcattc ccagagtttt
      421 atgctcccag cgggcgagcg cctcgaagcc ttcgatttta tcggtctcaa acgagtaaat
      481 cggctggtaa taaacgtcga attcctgttt ttcgatggcg tgacgcaaat cggtttcaat
      541 ctgcaaagtt tccttgacgg cgtcgcgcat atgctggtta aagatttcgt ggcgcgattt
      601 tccggcgcgt ttcgcctggt acatagcgat gtcggcgtcg cgcatcacgt cttcgggttt
      661 cgtgtattta tccgaagcgt gcaggatgcc gatgctcgcc gagctgaaaa tttcgtgacc
      721 gcttaaattg aaaggactca aaaacttttc gcggatgcgc tcggctatgc ggacgacttc
      781 tttcgggtcg tattttcctt cgaccagaat cgtaaattcg tcgccgccga gccgcgccac
      841 catatcggac gggcgcaggc attcgcgcag gcgctcggcg attccgataa gcaattcgtc
      901 gccgatttga tgaccgaggc tgtcattgac gattttgaaa cggtcgaggt cgatgaaaag
      961 aacgctgact ttataagtcg gattatcgcg cactttttcc agcgcctgtt cgagccgcgc
     1021 cataaaatac gcgcgattcg gcaagcccgt caaagcgtcg tgcgtggctt tatactgcaa
     1081 ttgttcttcg gtcagctttt taacggtgat gtcctgaatc tgaaagatta aattcgggcg
     1141 ctcggcttta tggtcgctgg tcgcggaaac gctccacgaa acccatacga tttcgccgcg
     1201 cttattcaaa tatcgctgtt cgagctggca ggcgggaact tttccggtcg tcaattcgtg
     1261 aagcttgacg agcgtgtcgc ccaaatcgtc ttcgtaaatc atcgtctgaa actcggtggc
     1321 gagaaattcc tcctccgtgt agccgaggat ttccgaaagc gctttgttta ttttcagcca
     1381 tctgccgtcg ggcgaaacga tgccgatgcc gatcggcgcg taggtaaacg cgctgcgaaa
     1441 acgttcttcg gattcggcga gcgcgcgcga ttgttcctcc agaatcaaag cgtgctgttc
     1501 ggcttgctcg gcttgcgaga gcgacatttc cacgtttttc agatacatcc ggtaggtcaa
     1561 ataaacgata acgatgaccg gcgcggcggc gattattacg ccgaaaccga tgacgaagac
     1621 cagctcggct aaaattccgg cggcgatcgc cccgacaaaa tacgtaatcg aagtccagag
     1681 ataatgcgtc ttccaggttt cccaccaggg tttttcgctt tttaatgcgc cgtaaatcga
     1741 ggctagaccg gaattgaaaa taaactgcac cagcgccatt acggacaggg aaattatcaa
     1801 attagagtgt gttttctcgt gaataaccgt cgcgccctcg atctcgaaag agcgcagaag
     1861 ggagacgacg aacgtcgtcg agcacgccag cagcgcggcg ttaaagaaaa ccgagatttt
     1921 tttcttgcag aagcgccacg aagagaaaaa agcctcgacc gccgaaagca aaatcgccgc
     1981 ttcgccgccg aaaagcagaa ttgccaggaa aataaacgtg tcggaaacgg cgatatgcga
     2041 tttaaaacgc ggaatctgaa tcgagatgcg tgagccgaaa gcgatggtca gcagagaaag
     2101 aaagacaaat tgcagagaca gtttttccgg cggaaaggtc cacaacgcga tggctaaaca
     2161 cagcgctccc aatgctatga tagccagcat atactgtctt gttgtttttt gagaatccat
     2221 acgcagttga aacggctcag acgatcgggg aatatttcat tttgggcggg agtgtattct
     2281 gtatttttgc ggaagcttcc cggagcaaaa accggcgctt caaataatta gtgcggaaag
     2341 gcttaacaaa aagccttccg atgattataa gcaataacaa aaaaattgtc tatagttttt
     2401 ctttgttaaa caaaaatcgt cgtacaactg tcttttgatt tgaacccggc tttcaaatat
     2461 gcggtatgag cctccttaaa atccacgttg cttaataaac acacgtgttt gacgcggttt
     2521 aaaagcagac ggctgagttg cgatagatat ttggaaccgg cgcccgcgcc gcgccgttcg
     2581 ggcgcgacat agatgccttc cagataaata acgtcaacgg tttcggcgac aatatcggct
     2641 ttgaaaagca atttgccgtt ttcgaagacg acaaaagttc tttcctgccg gattctgcgc
     2701 agagttcttt gaagaaagcc ttcgcggtct ttttccaaag ggttaatgcc gctctccatc
     2761 agagcgactt cggcgtgggc ttcggcaact tcgagcagtt cgtcttcggc tgccagcctc
     2821 aaatcttcag tcgtttcacc aaccataaca ggaaagctga tttcaaataa taattcgttg
     2881 cagattagtc gcggctggcg cgcgccgccg gaataatatt gccagaacaa ttcggcgctg
     2941 ttgccgtctg acataataat gtgaatcggc gtttcgcttt cgccgccaga gaaagcgaaa
     3001 gccttcagcg attcttccga gcgcgcctcg accagcgtcg tgtgcccgat aagagcgacg
     3061 ccttcgagtt ttccgtcgcc gtcgcggaag ccgtaaaatc tgccgcgatt gagcgagctg
     3121 tccagtccgt tgtcgttaat gaagctcgtc atcacgatgg tatgaaccgg gcgcattgcc
     3181 aggaaagcca gcgcttcggc ggtgtcggta tctttcagtt cgcagatttg cgccatatcg
     3241 ggcgcgagcg tgacggcgat tcgagccgca ggcagactct ggaccgtatg tctgttaaca
     3301 atctggtatg acattattat agtttcttta aattttttgg atttttggga gttgtggaac
     3361 tat
//
LOCUS       DASWNE010001082         5891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_422232, whole genome shotgun sequence.
ACCESSION   DASWNE010001082 DASWNE010000000
VERSION     DASWNE010001082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5891
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422232"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..440
                     /locus_tag="VGB68_20640"
     CDS             90..440
                     /locus_tag="VGB68_20640"
                     /inference="COORDINATES: protein motif:HMM:NF013234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HEY0051718.1"
                     /translation="MINEFDQRPWGNYTVLDEGEGYKVKRIEVLPKKRLSYQKHQRRA
                     EHWFVVSGNAKVTLNDREILVKTGESLDISIGDKHRVENAEEDLLIFIEVQKGDYLGE
                     DDIERFDDDFGRAA"
     gene            475..2721
                     /locus_tag="VGB68_20645"
     CDS             475..2721
                     /locus_tag="VGB68_20645"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HEY0051719.1"
                     /translation="MLYTLERQISFILLIAILLLIGIGIFAYYNSSLVQDSVKLEKRT
                     QEVLQKTDETLLFAVEAETDVRGFIITGRQEFADSYKTSRSKVMESLPQLKKITGENN
                     DNPGQTARIETLERQIGEKLNYLDRALEMRQSQGREVVIAMMGNGQGKILMDRARATT
                     QEIKNEENVLLEQREYELNQNLNRAFIFLLSSIVIGIMMLALAEFVVMREIGRRKKAE
                     IQLKEANRGLERRVEERTRQLSESELFSRTILNSLSANIAVLDKNGKIVAVNNSWEDF
                     AKLNSAAEQVPKTGVGENYLAVCAADSDEALSSVAEQLKDVLDRKSDGFSLEYPCDSP
                     DAERWFLMQVNPFHGAAGGAVVSHIDITDRKKAETELAASEEFNRSIVENSPDCVKVL
                     ELDGNMISMNYPGMCLMEIDDSELFRGELWTNNWEGEYNRLAREAVEKAKRGESAFFQ
                     GFARTAKGTPKWWDVTVAPVRDASGRVSRLLVNSRDITERKQAESELEKLLLSEQSAR
                     REAEIANRLRDEFMATVSHELRTPLNAILGWARLLKQGKLNEEMAGKAIETIIRSSET
                     QNRLIEDLLDVAKIVSGKLHLEKIEIAPDDLINNALATVKPSADAKSIRVNLNFDENR
                     DGKKISGDPNRLQQVIWNLLANAIKFTPEGGAIDVDLTGNDGFAEIKITDNGIGIKPE
                     FLPFVFERFRQDAANINRSGGLGLGLAIVRHLTEMHGGTVSASSGGENKGSTFTVKLP
                     TVSANGKI"
     gene            complement(2794..3339)
                     /locus_tag="VGB68_20650"
     CDS             complement(2794..3339)
                     /locus_tag="VGB68_20650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY0051720.1"
                     /translation="MKQKAVFVDRDGTLIEEVNFLSRVEDLRFFSFTDEAVRRLKEND
                     FLVVVVTNQSGIGRGIFTEAAMHEIHAAVQAKMTKKIDAFYFCPHLPDEGCACRKPKT
                     AMIRRAAADFKIDLENSWFVGDKAIDVETGQNAGLKTALVLTGYGRGATEQLENRPNL
                     TVENLLEAVKEILASEKHRRE"
     gene            complement(3441..4508)
                     /gene="waaF"
                     /locus_tag="VGB68_20655"
     CDS             complement(3441..4508)
                     /gene="waaF"
                     /locus_tag="VGB68_20655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEY0051721.1"
                     /translation="MKIVVRGTNWIGDAVMTIPALRELRRIFAGAHITLCTRGWAEGI
                     FADADFIDQILPFDKANNSFQTVFRQAKIWREKNFDLAVLFTNSFESALLGKLGKASR
                     RFGYATEKRSFLLTESFAVPAWKNSRHEIFYYLNLVAEIEKSLSGSTKVWENEPLFQL
                     NVSEKRRIEAREILKSQGVDLSRKTVALGVGSTNSRAKRWQAESYAKLNDRLQTELNA
                     NVILVGAADEIEVSVEVAEKANLKPIVLTGKTNLSQAVAVLSEIDLLIANDMGLAHVA
                     PATGTKTLVVFGPTNDKTTRPVGSEIIRKNVECSPCMLRVCPIDHRCMTRITPDEVFE
                     IAERTLNAKEEFATETQRFLK"
     gene            complement(4597..5448)
                     /locus_tag="VGB68_20660"
     CDS             complement(4597..5448)
                     /locus_tag="VGB68_20660"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY0051722.1"
                     /translation="MKVLVTGGSGFLGTHVRRFFAADDFSRRSNLDLLNLQDTQIVQD
                     YDAVIHLAAHLDKSPEAAEEVFLTNVEGTINLLRSMSPNAVFIFASTKDVYGRFADNQ
                     QLVSESCPTLYAGQSALEWSKLIAERYVDFYAHARGFRSCVFRLSTVYAPSSEGNTPN
                     FVTHYADMINKGERLRLPAGGKPRRDLLHVDDFSEACRAFIDSVINHGLYNLGGGFQN
                     ALALRELVGKMEEVSGYQALIDEENPLPAPVPVNYVTDLTRVRQELDWSPKISIEDGL
                     KTLFQTG"
     gene            complement(5610..>5891)
                     /locus_tag="VGB68_20665"
     CDS             complement(5610..>5891)
                     /locus_tag="VGB68_20665"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HEY0051723.1"
                     /translation="LKPTIADFMDSIVAETLDLVFEEVAIKSGSGYVGKCLKETNIRA
                     ELNLVIVAIRRKSGEMTFQPSGEDCIEENDLLIVIGRAESVAKLLKANR"
BASE COUNT         1544 a         1491 c         1515 g         1341 t
ORIGIN      
        1 aaagtattta tcgtttgtct gcattaagca tttaacattt aagatttatc atttatcatt
       61 aaatcaatct gttaaatggt aaatgataaa tgataaatga atttgaccag cgcccttggg
      121 gaaattatac cgttctggac gaaggcgaag gctacaaagt gaaaagaatt gaagttttgc
      181 cgaaaaagcg tttgagctac cagaaacatc agcgccgcgc cgaacactgg ttcgtcgtca
      241 gcggcaatgc aaaggtaaca ttaaacgacc gtgaaattct agtcaaaacg ggtgaatctc
      301 tggatatttc catcggcgat aaacaccgcg tcgaaaatgc ggaagaagat ttgctgattt
      361 ttatcgaagt tcaaaagggc gattacctgg gcgaagacga catcgagcgc tttgacgacg
      421 atttcgggag agccgcttaa aaggcgcagg ttttttcttt ttcgcctgaa aataatgctt
      481 tacactctcg aaagacaaat ctcgtttatt ctgctcatcg cgatactgct tctgatcggc
      541 atcggaattt ttgcttacta caacagttct ctggtgcagg attcggtcaa actggaaaaa
      601 cgcacgcagg aagttcttca gaaaacggac gagacgctgc tgtttgcggt cgaagcggaa
      661 acggacgtgc gcggctttat catcaccggc agacaggaat ttgccgattc ctataaaacg
      721 tcgcgctcga aagtgatgga aagcctgccg caattaaaaa aaataaccgg cgaaaataac
      781 gataatccgg ggcagacagc cagaatcgaa acgctcgaaa ggcaaatcgg cgagaaatta
      841 aattatcttg acagagcgct cgaaatgcgg cagagccagg gacgcgaagt cgtcatcgcg
      901 atgatgggca acggtcaggg caaaatttta atggacaggg cgcgcgcgac gacgcaggaa
      961 ataaaaaatg aagaaaacgt tttattggag cagcgcgaat acgaattaaa tcaaaattta
     1021 aatcgcgcgt tcatctttct tctgtccagc atcgtcatcg ggattatgat gctggcgctg
     1081 gcggaattcg tcgtgatgcg cgaaatcgga agacgcaaaa aagccgaaat ccagttaaag
     1141 gaagccaaca ggggtttgga gcgccgcgtc gaagagcgca ccagacagtt gagcgaaagc
     1201 gagctttttt cgcgcacgat tttaaactcg ctgtccgcca atatcgccgt gctggataag
     1261 aatggaaaga tcgtcgccgt caacaactcg tgggaagatt tcgcgaaatt aaattccgcc
     1321 gccgaacaag tgccgaaaac cggagtcgga gaaaattatc tagccgtctg tgccgccgat
     1381 tcggatgaag ctttgagctc ggtcgccgaa caattaaaag acgttctgga cagaaaatcc
     1441 gacggcttca gcctcgaata tccgtgcgac tcgccggatg cggagcgctg gtttctgatg
     1501 caggtcaacc cgtttcacgg cgcggcgggc ggcgcggtcg tctcgcacat cgacatcacc
     1561 gaccgcaaaa aagcggaaac cgagcttgcc gccagcgagg aatttaaccg gagcatcgtc
     1621 gaaaacagcc ccgattgcgt taaggttctc gaactcgacg gcaatatgat ttcgatgaat
     1681 tatcccggaa tgtgtctgat ggaaattgac gattccgagc tttttcgcgg agagctttgg
     1741 acgaataact gggaaggcga atacaaccgg ctggcgcgcg aagcggtcga gaaagcgaaa
     1801 cgaggcgaat cggcgttttt tcagggtttc gcccgcacgg ctaagggaac gccgaaatgg
     1861 tgggatgtga cggttgcgcc ggtgcgcgac gcgagcggca gagtctcccg gctgcttgtc
     1921 aactcgcgcg acatcaccga gcgcaaacaa gccgagagcg agctggaaaa actgcttttg
     1981 agcgaacagt cggcgcgccg cgaagccgaa atcgccaacc gcctgcgcga cgaatttatg
     2041 gcgacggtct cacacgaatt gcgcacgccg ctgaacgcca ttttaggctg ggcgcggctt
     2101 ttgaaacagg gcaaattaaa tgaggaaatg gcgggcaaag cgattgaaac gattattcgc
     2161 agctccgaaa cgcagaatcg cctgattgaa gatttgctcg acgtggcgaa aatcgtctcc
     2221 ggcaagctgc atctggaaaa aatcgagatt gcgcccgatg atttaatcaa taacgcgctg
     2281 gcgaccgtca agccttccgc cgacgccaaa tcaatcaggg tcaatttaaa tttcgacgaa
     2341 aacagggacg ggaaaaaaat ttccggcgac ccgaaccggc ttcagcaggt catctggaac
     2401 ctgctggcaa acgccataaa atttacgccc gaaggcggcg ccatcgacgt cgatttaacc
     2461 ggcaatgacg gcttcgccga aatcaaaatc accgacaacg gcatcggcat caaacccgaa
     2521 tttctgcctt tcgtcttcga gcgtttccgg caggacgcgg caaacatcaa tcgaagcggc
     2581 ggcttgggtt tgggcttggc gattgtccgg catctgaccg aaatgcacgg cggaacggtc
     2641 agcgcatcga gcggcggcga aaacaaaggc tcgaccttta ccgtaaaact gccgacagtt
     2701 tcggcaaacg gaaaaattta agcgcagagt acgcagagct taaagagaaa gaataaatcg
     2761 agcggttaat ttgttcgcgt ttacttgcgg gtttcattct ctccggtgtt tctccgaggc
     2821 taaaatttct ttcaccgctt cgagcagatt ctcgaccgtc aaattcggtc gattttccag
     2881 ctgttcagtc gcgcctctcc cgtaacccgt caaaacgagc gcggttttca gcccggcgtt
     2941 ttgcccggtt tccacgtcaa tcgctttatc gccgacaaac cacgaattct ccaaatctat
     3001 tttaaaatcg gcggcggcgc ggcgaatcat tgcggttttc ggtttgcggc aagcgcagcc
     3061 ttcgtccggc agatgcgggc agaaataaaa agcgtcaatc tttttcgtca tcttcgcctg
     3121 gacggctgcg tgaatctcgt gcatcgccgc ttcggtgaaa attccgcgcc cgatgccgga
     3181 ttgattggtc acgacgacga ccagaaaatc attttccttc agccgcctta ccgcttcgtc
     3241 ggtaaaagaa aaaaatcgta aatcttccac gcgcgaaaga aaattaactt cttcgataag
     3301 cgtgccatcg cggtcaacga aaacggcttt ttgtttcata ggtttttaac gcaggaaaga
     3361 tttaaaccac cgagacaagg agacacagaa aaaaactaaa tcaaaaataa atctgcttcc
     3421 atctgtgtaa atctgtggtt tcattttaaa aatctctgtg tctcggtggc gaattcttcc
     3481 tttgcgttta atgttctttc ggcaatctca aaaacttcgt ccggcgtaat gcgcgtcata
     3541 cagcggtggt caatcggaca cactctgagc atacacggcg agcattcgac gtttttccga
     3601 atgatttccg agccgaccgg gcgcgtcgtt ttgtcgtttg tcggaccgaa gacgaccagc
     3661 gttttcgttc cggtcgcggg cgcgacgtgc gccaatccca tatcgttggc tatgagtaaa
     3721 tcaatctcgg acaagaccgc gaccgcctgc gacagattcg tttttccggt caggacaatc
     3781 ggttttaaat tcgctttttc ggcgacttcg acggaaacct cgatttcatc cgcggctccg
     3841 accagaatta cattcgcgtt taattccgtc tgcaagcggt cgttcaattt cgcgtagctt
     3901 tccgcctgcc agcgtttggc gcgcgaattg gtcgagccga cgccgagcgc gacggttttc
     3961 cgcgacaaat cgacgccctg acttttcagg atttcacgcg cctcgattct tcttttctcc
     4021 gaaacattca actgaaaaag cggttcgttt tcccagactt tcgtgctgcc ggaaagtgat
     4081 ttttcgattt cggcgaccag attcaaataa taaaaaattt cgtggcgcga gtttttccag
     4141 gcgggaacgg caaaagattc ggtcaaaaga aaagagcgtt tttcggtcgc gtaaccgaaa
     4201 cgcctcgaag ctttgccgag ctttccgagc aacgccgatt caaacgagtt cgtgaataag
     4261 acggcgaggt cgaaattttt ctcgcgccag attttcgcct gccggaaaac ggtctgaaac
     4321 gaattattcg ctttatcaaa cggcagaatc tggtcgataa aatcggcgtc ggcaaagatt
     4381 ccttccgccc agccgcgcgt gcaaagcgta atatgcgcgc ccgcaaaaat ccggcgcagc
     4441 tcgcgcaaag ccggaatcgt catcaccgca tcgcctatcc aatttgttcc gcgcacgact
     4501 attttcatag ggaaattcaa acaggatata caggatttac aggatataaa gaaaatattc
     4561 gatgtgaatt ttgcttaata atcctgccta tcctgtctat cctgtttgaa ataatgtttt
     4621 taatccgtcc tcgatagaaa ttttcggact ccaatccagt tcctgccgga ctctggttaa
     4681 atccgtcacg taattgaccg gcacgggcgc gggcagagga ttttcctcgt caatcagcgc
     4741 ctgataaccg gagacctctt ccattttgcc gacgagctcg cgcaaggcga gcgcgttttg
     4801 gaaaccgccg ccgagattgt acagcccgtg attaatcacc gaatcgatga aagcccggca
     4861 ggcttcggaa aaatcatcga cgtgcagcaa atcgcggcgc ggcttcccgc cggcgggaag
     4921 ccgcagccgc tcgcctttat ttatcatatc ggcgtaatgc gtgacgaaat tcggcgtgtt
     4981 gccttcgctc gaaggcgcgt agacggttga aagacggaaa acgcacgagc gaaaaccgcg
     5041 cgcgtgcgcg taaaaatcga cgtaacgctc ggcaatcaat ttcgaccatt cgagcgccga
     5101 ctgcccggcg taaagcgtcg ggcagctctc ggaaactaac tgctgattgt cggcgaatct
     5161 gccgtagacg tctttggtcg aagcaaaaat aaaaaccgcg ttcggcgaca tcgagcgcaa
     5221 aagattgatt gtgccctcga cgttggtgag aaaaacttcc tcggcggctt cgggcgattt
     5281 atcgagatgc gcggcgagat gaatgaccgc gtcgtaatcc tgcacgattt gcgtgtcctg
     5341 caaattcagc aaatccagat tcgagcgccg cgaaaaatcg tccgccgcga aaaaacggcg
     5401 aacgtgcgtg ccgagaaaac cactgccgcc cgtaacgaga actttcatac cggaatttta
     5461 acgcaaaggc gcaaggacgc gaagctggcg ttcgagatag acaatcgatt tgaagctttt
     5521 cgcccgcctt gtcttttacg acaaacgatt tggcgagcac gctcgccaac gtccgtgtaa
     5581 aatgcgcgga gaggcgtcag cgccgccggt tagcggtttg ctttcaggag cttcgccacc
     5641 gattcggcgc gtccgatgac aatcagcaaa tcgttttctt cgatacagtc ttcgcccgaa
     5701 ggctggaaag tcatctcgcc cgatttgcgg cgaatggcga cgataaccag attcagctcg
     5761 gcgcgaatgt tcgtctcttt cagacatttg ccgacataac cggagccgct tttaatcgcc
     5821 acttcttcaa acaccaaatc gagcgtttcg gcgacaatcg aatccataaa atcggcgatg
     5881 gtcggtttta a
//
LOCUS       DASWNE010001083         1504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_635078, whole genome shotgun sequence.
ACCESSION   DASWNE010001083 DASWNE010000000
VERSION     DASWNE010001083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635078"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..673)
                     /locus_tag="VGB68_20670"
     CDS             complement(59..673)
                     /locus_tag="VGB68_20670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0051724.1"
                     /translation="MRTAAANTLLTSDLTDSAENATPDKAVARNAELEELAGLFHDYH
                     KAIFRIAYRITGSQSDAEDVLQTIFLRLTPNRRDLAPNPEGYLHRAAVNASLDLLRRR
                     ARASFVALDVMDFNQSSKLSAPSPEDDFADAELRELIRHAVAKLEGRAATAFALRYFE
                     GYDNARIAQVLGTSQMVVAVTLHRARTRLRKEIGNYLEKHHEAQ"
     gene            complement(798..>1504)
                     /locus_tag="VGB68_20675"
     CDS             complement(798..>1504)
                     /locus_tag="VGB68_20675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051725.1"
                     /translation="TAIALCNNMAIFPSVIAEKLADIYLAGHLKPVAPSRKTVAEPLP
                     QAVALSEKEALRYVGIYAHPESGRVFRLSFRDGKLINSGIFKNEIAVTPVSENRLLVV
                     ESNGMAELNPVFDKSGTISEIRALNKSGKPDIFVPVKPPFDSPQQLSEYAGTYYSDEF
                     DADYKLTLQGNNLVLQIGENFEERLTAAYADYFTIRGGAVNLSFTRDDKGKIAGFVFN
                     SAIDERDVKGIAFKRQ"
BASE COUNT          318 a          404 c          392 g          390 t
ORIGIN      
        1 gagctgctcg tcgcgtatgc cgcgcgcggc gtcgtctatg attgcgtcca actcttgttt
       61 attgtgcttc atgatgtttc tccaaataat tgccgatttc tttgcgaagg cgtgttctgg
      121 cgcggtgcag cgtgaccgcg acgaccattt gtgaggtgcc taaaacctgt gcgatgcgcg
      181 cattgtcgta gccttcaaaa taacggagcg cgaaggcggt cgcggcgcgc ccttcgagtt
      241 tcgcgaccgc gtggcggata agctcgcgca gttccgcatc cgcaaaatcg tcttcgggac
      301 tcggagccga gagcttcgag ctctgattga aatccatgac atccaaagcg acgaaactgg
      361 ctcgcgcgcg cctcctgagc aggtcgagcg aggcgttgac cgccgcccgg tgcagatagc
      421 cttccgggtt cggcgctaag tcgcgccggt tcggagtgag ccgcaggaaa atagtctgca
      481 acacgtcttc ggcgtcgctc tggctgcccg taatacggta cgcgatgcgg aagatcgctt
      541 tatgatagtc gtgaaaaaga ccggcgagtt cttcaagctc cgcgtttcgc gcgaccgctt
      601 tatcgggcgt cgcgttttct gccgagtctg ttaaatcgct tgttagtagt gtgtttgcgg
      661 cggcggttct cacggttgtc tcctttgaat cgtcatctac aaatataaac gcgcgcggcg
      721 gctgattatt aacagcgaag agaaataaat tttcatatcc cttgtaaata ttttaatttc
      781 agcccgcttt caataatcta ctgtcgcttg aaagcgattc ctttaacatc ccgctcgtcg
      841 attgctgaat tgaacacaaa tcccgcgatt tttcctttat catcgcgagt aaaagaaaga
      901 ttaaccgcgc cgccccgaat ggtaaaataa tcggcgtatg cggcggtcag ccgctcttcg
      961 aaattttccc cgatttgcag gacgagatta ttgccttgca gcgttaattt gtaatccgcg
     1021 tcaaactcgt cgctgtaata agtcccggcg tattcggaca attgctgcgg cgaatcgaac
     1081 ggcggtttga cgggcacgaa aatatccggc tttccgcttt tgttaagcgc ccttatttca
     1141 gaaattgtac cggacttgtc aaagacggga tttagctccg ccattccgtt actctcgacg
     1201 accagcagac ggttttcgga aacgggcgtc accgctattt cgtttttgaa gattccgctg
     1261 ttaattaatt ttccgtccct gaaactcagc ctgaagaccc tgccgctctc cgggtgcgcg
     1321 taaatgccca cgtagcgcag cgcttctttt tcagacagcg cgacggcttg cggcaaaggc
     1381 tccgccactg ttttccggct cggcgcaaca ggtttcaagt gtcccgccag ataaatgtcg
     1441 gcgagtttct ccgcaattac actcggaaaa atcgccatat tgttgcacaa agcgattgcc
     1501 gtaa
//
LOCUS       DASWNE010001084         3200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_350900, whole genome shotgun sequence.
ACCESSION   DASWNE010001084 DASWNE010000000
VERSION     DASWNE010001084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350900"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VGB68_20680"
     CDS             <1..501
                     /locus_tag="VGB68_20680"
                     /inference="COORDINATES: protein motif:HMM:NF045201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HEY0051726.1"
                     /translation="TVDTTVVFPATVYFEGTQISEGRGTTRPFEIIGAPFINSKDYAE
                     ALNLLELPGVTFRAVEFLPTFQKHVGVTCGGVFVHVLDRHIFRPVITGAALVKTCFDL
                     YGSEFKWKNPPYEYVFDRNPFDVIAGTTRLRESFEQGIELSEIESSWKKDENAFRGLR
                     EKYFLY"
     gene            622..1005
                     /locus_tag="VGB68_20685"
     CDS             622..1005
                     /locus_tag="VGB68_20685"
                     /inference="COORDINATES: protein motif:HMM:NF042755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6677 family protein"
                     /protein_id="HEY0051727.1"
                     /translation="MGNYILTGIIAWFLPGVGHLYLGRWRRGLIIGAVIWIMFIIAII
                     GGGAYYPGYSFKDGLLLYVLNIFARLGNGLGLLTSLSLTFNQPATNAASWATFEYAGR
                     FLEVAGLLNYLAVIDAVDISLGRKK"
     gene            1002..1169
                     /locus_tag="VGB68_20690"
     CDS             1002..1169
                     /locus_tag="VGB68_20690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051728.1"
                     /translation="MIHFLYLIGFALLVAAAFAAFHSGSTRERFLYGLKVFAQFVVIS
                     LVLAWVFYFIP"
     gene            1183..1935
                     /gene="recO"
                     /locus_tag="VGB68_20695"
     CDS             1183..1935
                     /gene="recO"
                     /locus_tag="VGB68_20695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEY0051729.1"
                     /translation="MAIFETESLILKTYPLAEADKIVVFLTQTNGLVRGVAKGAKRLK
                     SRFGGGLEPFSIVNATFYQKEERELVSISQIELQKSFFESASNPLFLQKFAYLAELLS
                     EFVPPHEPNERIYRMTRICLETAAEHPEFLENVALYFEIWLLRLGGYLPSWERCDNCK
                     RQFGASEKASLQINFHLLCENCQPVKNRNIIGADLRKIYSVAQTLSPVRFAALTEGKI
                     EEIKEVSDILKRLITHILGKELKNDKILVAGL"
     gene            1950..>3200
                     /locus_tag="VGB68_20700"
     CDS             1950..>3200
                     /locus_tag="VGB68_20700"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEY0051730.1"
                     /translation="MNLFFKLISKYVIQAVLPYFLFTWILLSVILFVQQASRYSDIFF
                     NANLPTGLIWQLTFALVPSVIAFTCPMAILVGVIIGLAKMQGDSELTAIRAAGVGNFQ
                     IVLPIVFLGLILSAFAFLINWRGVPLAAQVVRKVALQTALFKMESPIEPGVFNTEIRG
                     YTVYVKSGDILKGTWEKIFIFNEDKQTGQMRLITSKNGKIDSNDDDSELVLDEALVST
                     FTPSDASKPIFFDKIKNLRFVIQTKRGEIIERLSKTEENADELGLSELAAFARKKEGK
                     ERTEAEILWQRRIILSVTPLLFALLGASLILRFNRGGRGFGIFLALGSLIGYYLLALM
                     GEQLARTNKLSVLGGGTIPIAVTVALIIWFNFSQKIGSTRRFMIFSRFKESIPLRFEN
                     NSNKRSFPNLSTGILDFDIVSNLVK"
BASE COUNT          799 a          713 c          785 g          903 t
ORIGIN      
        1 acggtcgata caaccgtcgt ctttccggcg acggtttatt ttgagggcac gcaaatttcc
       61 gaaggacgcg gaacaactcg cccgttcgaa attatcggcg cgccttttat caactcgaaa
      121 gattacgccg aagctctgaa cttacttgag cttccgggcg taacctttag agccgttgag
      181 tttctgccga cgtttcagaa acacgtcggc gtcacctgcg gcggcgtctt cgtccacgtt
      241 ttagaccgtc atattttcag accggtcatt accggcgcgg cgttggtcaa aacctgtttt
      301 gatttatacg gttcggagtt taaatggaaa aatcctccgt atgaatacgt tttcgaccgc
      361 aatccgtttg acgtcatcgc cggaacgacc cggctgcgcg aaagtttcga gcagggaatt
      421 gaattgagcg agattgaaag ctcctggaag aaagatgaaa atgcatttcg cggcttgcgc
      481 gaaaaatact ttctttacta aagcttaaga gttactttaa gcaggtaaat gtattataat
      541 agaatattta cagggcaaaa ccaaaatagg attttaagcg tcggcgagcc gacagttaaa
      601 ttaacagttt aaataaaaac gatgggaaac tatatcttaa cgggcatcat cgcatggttt
      661 ctacccggtg tcgggcattt atatctggga cgctggcggc gcggcttaat aatcggcgcg
      721 gtcatttgga ttatgttcat tatcgccatt atcggcggcg gagcatacta tccgggttac
      781 agctttaagg acgggctttt gctttacgtg ctgaatatat ttgcccgtct cggtaatggc
      841 ttaggcttgc tgacgagctt gtctttaacc tttaatcaac cggcgacgaa tgccgcttcg
      901 tgggcgactt ttgaatacgc gggcagattt ctggaagttg ccgggctgct taattatctg
      961 gcggtaatcg atgccgtcga tatttctctg ggacgaaaaa aatgattcat tttttatatt
     1021 taatcggttt tgcgcttctc gtcgcggcgg ctttcgcggc gtttcattcc ggcagcacgc
     1081 gagagcggtt tctttacggt ttaaaagtat ttgcccagtt tgtcgtgata agtctggttc
     1141 tcgcctgggt attttatttt attccttaat atttgagttg taatggcaat atttgaaacg
     1201 gaaagcttaa ttttaaaaac atatccgctg gcggaagcgg ataaaatcgt cgtttttctc
     1261 acacagacta atggacttgt gcgcggcgtg gcaaagggcg cgaagcgtct gaaaagccgt
     1321 ttcggcggcg gacttgagcc cttttcgatt gttaacgcga cgttttatca aaaggaagaa
     1381 agagagctcg tctcgataag tcaaatcgaa ttgcaaaaat cgttttttga atcggcgagc
     1441 aatccactgt ttctgcaaaa gtttgcttat ctcgccgagc tcctgagcga attcgtgccg
     1501 ccgcacgagc cgaatgaaag aatctatcga atgacgcgca tctgtctgga gactgccgcc
     1561 gaacatccgg aatttctcga aaacgtggcg ctttattttg aaatctggct gctgcgtctg
     1621 ggcggttatc tgccttcctg ggaaagatgc gataattgca aaagacaatt cggcgcgagc
     1681 gagaaggcga gcttgcaaat caatttccac ctgctttgcg aaaactgtca gcccgttaaa
     1741 aacaggaata tcatcggcgc cgatttaagg aaaatatatt cggtcgcgca gacgctctcg
     1801 cccgtcaggt tcgccgcgct gacggaaggc aaaattgaag aaataaaaga agtttcggat
     1861 attttaaagc gacttataac gcatattctg ggaaaagaat taaagaatga taaaattctc
     1921 gtcgccggtt tataaaccgc tccaaaaaga tgaatctttt cttcaagcta atctcgaaat
     1981 atgtcataca ggcggttctt ccatactttc tcttcacctg gattttgttg agcgtcattc
     2041 ttttcgtcca gcaagccagc cgttattccg acattttttt taacgccaat ctcccgaccg
     2101 ggctcatctg gcagctgact tttgcgctcg tgccgagcgt gattgccttt acctgcccga
     2161 tggcaattct ggtaggcgtc atcatcggct tggcaaaaat gcagggcgac agcgagctga
     2221 ccgcgattcg cgctgccggc gtaggcaatt ttcaaatcgt tctgccgatt gtctttttag
     2281 gtttgattct ctccgctttt gcttttctga ttaactggcg cggcgttccg ctggcggcgc
     2341 aggtggttcg aaaagttgcg ctgcaaaccg cgctttttaa aatggaatcg ccgattgagc
     2401 cgggcgtttt taacacggaa attcgcggct atacggttta tgtaaaatca ggagatattc
     2461 tcaaaggcac ctgggaaaaa atctttattt ttaatgaaga caaacagacc ggacaaatgc
     2521 gtctgataac gtctaaaaat ggaaaaattg attcaaatga cgacgactcg gagctggttc
     2581 tggatgaagc gctggtttca actttcacgc ccagcgatgc gagcaagccg attttttttg
     2641 acaaaatcaa aaatcttcgc tttgtcattc agaccaaacg cggcgagatt atcgaaaggt
     2701 taagtaaaac agaggaaaac gccgacgagt tgggcttatc ggagcttgcc gcttttgccc
     2761 ggaaaaaaga agggaaagag cgtacggaag ccgagatttt gtggcagcgg cgcattattt
     2821 tatccgtcac gccgcttctt ttcgctcttt tgggcgcgtc tctgattctg cgttttaacc
     2881 gcggcggcag aggtttcgga atttttctcg cgctcggcag cctgataggt tattatctgc
     2941 tcgcccttat gggcgagcaa ttggcgcgca cgaataaatt aagcgtttta ggcggaggca
     3001 cgattcccat cgccgtcacg gtcgcgctga taatctggtt taatttttcg caaaaaatcg
     3061 gttcgacgcg ccgatttatg attttttcgc gttttaaaga aagcattccg ctacggtttg
     3121 aaaacaattc gaataaacgc agctttccta atctttccac gggaatttta gatttcgaca
     3181 tcgtttccaa tctcgtcaag
//
LOCUS       DASWNE010001085         3558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_350988, whole genome shotgun sequence.
ACCESSION   DASWNE010001085 DASWNE010000000
VERSION     DASWNE010001085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350988"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1924)
                     /locus_tag="VGB68_20705"
     CDS             complement(<1..1924)
                     /locus_tag="VGB68_20705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051731.1"
                     /translation="MFVSLVIILLITFSGLALTYLYDEDAPLLVRLGAGNVVGAALFS
                     LLVFIVGCFFGMTTATVLLSLALSLLPLGLLGREDFRKKFSANLSASVKTLDNAGWQK
                     IAGFAYYAALLLVLYFFFDRAMLETKDGIYTGGSQNLGDLPFHLGAIFSFTEGNNFPP
                     ENPSYAGAKFTYPFMADLATAALVKLGAGVREAMLIQNVTLGFSLVVLLERFARKLTG
                     SRLAGKITPLLLLFCGGAGFAIFLRDYWQDGRSFFEFLWNLEKDYTIRPEGLRWGNAL
                     VVLFITQRSLLLGLPITLIVLTKLWEFFNSDAGMRRQGDAETERRGEAETEIAGSPGR
                     RVAATVFVGLLAGTLPLIHVHSLAVLFLVCAVLFFFSPEKWKHWMAFGIAVSIVAVPE
                     LIWVMTGSATRLSEFIGWHFGWSAGKENYFVFWAKNLGLFIPLLVIALFLWSQRRQTD
                     AAGEKNDERILPLKPNTENRLLIFYIPFALCFVISNLVKLAPWEWDNIKVLIYWFVAS
                     APLVAWLLVRLWEKGAAFKIVSAACLAALIFSGALDVWRAISGQINYQVFSRDAVAIA
                     EQIKQKTPPGALFLNAPTYNSAVVLAGRRSLMRYSGHLSSYGIEYEPRESEVKRIYEG
                     TALADAFLKKNNIEYVL"
     gene            2081..3181
                     /gene="ychF"
                     /locus_tag="VGB68_20710"
     CDS             2081..3181
                     /gene="ychF"
                     /locus_tag="VGB68_20710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEY0051732.1"
                     /translation="MLQAGIVGLPNVGKSTLFNALTAQEAALAANYPFATIEPNVGVV
                     AVPDERLAVLEKMNKAQRVVPATVEFVDIAGLVRGASKGEGLGNQFLANIRETDAVIQ
                     VVRCFEDENIVHVEGSVNPIRDIETIQIELALADLGSVEKRREKAGRAAKSGDKEARR
                     DLEIMDKIQPVLEDGRPVRSVDLSDEEKAVVKSWFLLTSKPTIYAANVDEDSLMNPDE
                     NPHVRTVKEHAAQERAETVVICAKLEAELVALEPEERGEFLKDVGVASSGVDSLIKSA
                     YRILGLMSFLTAGEKEVRAWTIPTGTKAPQAAGEIHTDIERGFIRAEIISYDDLVRVG
                     SEKAAKEQGLARLEGKEYVMQEGDVVNFRFNV"
     gene            complement(3322..>3558)
                     /locus_tag="VGB68_20715"
     CDS             complement(3322..>3558)
                     /locus_tag="VGB68_20715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051733.1"
                     /translation="DSAIRAENYYTDGFLMYQDLSYWGKHRMLKDKSETYSVEGGNAD
                     LRHYLARLQRSSRCFSRCFKALARAVELFVYFYN"
BASE COUNT          967 a          973 c          886 g          732 t
ORIGIN      
        1 ttaaaacata ttcgatgtta ttttttttca aaaaagcgtc cgccagcgcc gtgccttcgt
       61 agatgcgctt gacctccgat tcgcgcggct cgtattcgat gccgtaggag gaaagatgtc
      121 ccgaatagcg catcagggaa cgcctgcccg ccagcacgac ggcggaatta tacgtcggcg
      181 cgtttaaaaa taaagctccg ggcggcgttt tctgtttgat ttgctcggca atcgccaccg
      241 cgtcgcggct gaaaacctga taattgattt gtccggaaat cgcccgccaa acgtccagcg
      301 cgcccgaaaa aatcagcgcc gccaaacagg cagccgaaac gattttaaac gccgctccct
      361 tttcccagag ccggactaaa agccacgcga ccagcggcgc ggacgcgaca aaccagtaaa
      421 tcaaaacctt gatgttgtcc cattcccagg gcgccagctt gaccagattc gaaatcacga
      481 aacacagggc aaacggaatg taaaatatca ataaacggtt ttcggtattc ggcttcagcg
      541 gcaaaatccg ttcatcgttt ttttctccgg cggcgtccgt ctgccttctc tgcgaccaga
      601 gaaacaaagc gataaccagc aacggtataa acagtcctaa atttttcgcc caaaagacaa
      661 aataattttc cttgcccgcc gaccagccga aatgccagcc gataaactcg ctcagacgcg
      721 tcgcgctgcc ggtcatcacc cagattaatt ccggaacggc gacgattgaa accgcgatgc
      781 cgaacgccat ccaatgcttc catttttccg ggctgaaaaa gaataaaacc gcgcagacca
      841 gaaacaaaac cgccaggctg tggacgtgaa tcaaaggcaa agttccggcg agcaaaccga
      901 caaaaaccgt cgcggcgacc cggcgacccg gcgacccggc aatttccgtc tccgcctctc
      961 cgcgtctctc tgtttccgcg tctccctgtc tccgcatccc cgcgtcgctg ttaaaaaatt
     1021 cccagagctt cgtcaaaaca atcagcgtta tcggcaaacc caaaagcaga cttcgctgcg
     1081 tgataaacaa cacgaccagc gcgtttcccc agcgcaagcc ttcgggtcga atcgtgtaat
     1141 ccttttccag attccacaaa aattcgaaaa agcttctgcc gtcctgccag taatcgcgca
     1201 gaaaaatcgc gaatccggcg ccgccgcaaa acaataaaag caaaggcgtt atttttcccg
     1261 ccaaacggct gccggtcaat ttgcgcgcaa atctttccag caaaacgacg agcgaaaaac
     1321 cgagcgtcac gttttgaatc agcatcgcct cgcggacgcc cgctccgagc ttgaccagcg
     1381 cagccgtcgc caaatccgcc ataaacgggt aagtgaattt cgcgcccgcg tacgacggat
     1441 tttccggcgg aaaattgttg ccctcggtaa acgagaaaat cgcgcccaga tgaaacggca
     1501 aatcgcccag attctgcgag ccgcccgtgt aaatcccgtc tttcgtctcg agcatcgcgc
     1561 ggtcgaagaa aaaataaaga accaaaagaa gcgcggcgta ataagcgaaa cccgctattt
     1621 tttgccagcc ggcattatcg agcgttttca ccgacgcgct cagattcgcc gaaaattttt
     1681 tacgaaaatc ctctctgccc aataaaccca acggcagaag cgagagcgcc agcgaaagca
     1741 aaaccgtcgc cgtcgtcatt ccgaaaaaac agccgacgat aaacaccagc agcgaaaata
     1801 aagccgcgcc gacgacgttt cccgcgccga gacgcacgag cagcggcgcg tcttcgtcgt
     1861 aaagatacgt cagcgccaaa ccgctgaaag ttatgagtag aataattact aaagaaacaa
     1921 acataaataa tccgcgttcg tcgacgctaa cttatgagta attttccgtc aaaattaagc
     1981 cacaatttat cgatttctca aaattacggt taaaggttac aataatcgtt ttgaatattg
     2041 aacaaatatt cgaccattta tcattaacaa ttaaattttt atgttacaag ccggaatcgt
     2101 tggactgccc aatgtcggga aatccacttt atttaacgcg ctgacggcgc aggaagcggc
     2161 gctcgccgcc aattatcctt tcgccacgat tgagccgaat gtcggcgtcg tcgccgtgcc
     2221 ggacgagcgg ctcgccgttc tggaaaagat gaacaaggcg caaagggtcg tgcccgcgac
     2281 ggtcgagttc gtcgacatcg ccgggctggt gcgcggcgcg tcgaagggcg agggcttggg
     2341 caatcaattc ctcgctaata ttcgcgagac cgacgcggtg attcaggtcg tgcgctgttt
     2401 cgaagacgaa aatatcgtcc acgtcgaagg ctcggtcaac ccgattcgcg acatcgagac
     2461 gattcaaatc gagctggctc tggcggattt gggaagcgtc gaaaaaaggc gcgagaaagc
     2521 cggacgcgcc gcgaaatccg gcgacaagga agccagacgc gacctcgaaa ttatggataa
     2581 gattcagccc gtgctggaag acggcagacc cgtgcgcagc gtcgatttgt ccgacgagga
     2641 aaaggcagtc gtcaaaagct ggtttctttt gacctcgaaa ccgacgattt acgcggcgaa
     2701 tgttgacgaa gattccctga tgaacccgga cgaaaacccg cacgtccgaa ccgtcaagga
     2761 acacgcggcg caggaacgcg cggagacggt cgtgatttgc gccaagctcg aagccgaact
     2821 ggtcgcgctc gaaccggaag aacgcggtga atttttaaag gacgtcggcg tcgcgtcgag
     2881 cggcgtcgac agccttataa aatcggctta ccgcatcttg ggcttgatgt cttttctgac
     2941 ggcgggcgaa aaggaagttc gcgcgtggac gattccgacg ggaacgaaag cgccgcaggc
     3001 ggcgggcgag attcacacgg acatcgagcg cggatttatt cgagccgaaa tcatttccta
     3061 cgacgatctg gtgcgcgtcg gctcggaaaa agcggcgaaa gaacagggct tggcgcggct
     3121 cgaaggcaag gaatacgtca tgcaggaagg cgacgtggtg aatttccgtt ttaacgtctg
     3181 atgttttccg gttttcgcaa aagcaaatag tcccgtttat tgattgcagg atttaatatt
     3241 ggggagtgac taaataagtg ttggcagatg ttgcgccaga taagccggat atttcggtaa
     3301 acgtcttttt ctgagttgtc gctagttata aaaataaaca aataactcaa ccgctctggc
     3361 taatgcctta aaacaacgag aaaaacagcg tgatgagcgc tgcaagcgag ccagataatg
     3421 acgcaaatca gcattgccgc cttcgaccga ataagtctcg ctcttatctt tcagcattcg
     3481 atgcttgccc caataactca aatcctgata catcaaaaag ccatctgtat aatagttttc
     3541 cgcccttatt gctgaatc
//
LOCUS       DASWNE010001086         3757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_635539, whole genome shotgun sequence.
ACCESSION   DASWNE010001086 DASWNE010000000
VERSION     DASWNE010001086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635539"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VGB68_20720"
     CDS             complement(<1..466)
                     /locus_tag="VGB68_20720"
                     /inference="COORDINATES: protein motif:HMM:NF014860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0051734.1"
                     /translation="MPRRARLGLDRAGIETGSRGYIKVNDELRTNVPGIWALGDCNGK
                     NAFTHTSWNDAEIVAANLLDNEARRTSDRFPACGLFIDPPLGRVGMTEAEIRRTGRRA
                     LIGKRPMSRVSRAVEKGETEGFMKIAVDAETREILGAAILGTGGDEVVHSILD"
     gene            476..961
                     /locus_tag="VGB68_20725"
     CDS             476..961
                     /locus_tag="VGB68_20725"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY0051735.1"
                     /translation="MPEFLWQWIMTGLRRERRVLLFEGSPRVLGTFAEEFSKSAEHQL
                     RDLGVEVFLNSFVKQVEPNRIKVGEQWIECSVTLWATGVMASELGERSAAKTDRAGRV
                     LVEPDLSIPTYKNIFVIGDMAFLKDAGGVIVPGLSPAAIQEGEHAAKNILGDLIVVVD
                     A"
     gene            complement(1087..3210)
                     /locus_tag="VGB68_20730"
     CDS             complement(1087..3210)
                     /locus_tag="VGB68_20730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051736.1"
                     /translation="MPAKTVQFNVEIKGLEITSGAAQVRGIVSGQVGSAYNSSYFKGN
                     TFSAALPEDQSEYFAVTVFIKTYTLNGVSYNVEDKPLTLLATLYLPDADYVVKVSPES
                     SIASIYTFARMTTVQSDGLVTMSGSERNMKIAYGMKQNFYLTEGTVADMIKESPNGFE
                     TNSYPMFNSLCNLLYYCLTDTGFYNQFLDYAWHEENAPNNNFLQAVRNMLYDPFTNAD
                     NIYQMLVGKPEIYQDSLTSMNVSDDEKAPHNWTLTLKSNSSGAKNFIPSGLAFVVFDA
                     DDNAWVANNFRAGSNLSGTHCLVYKYNAQPADFSPVQGGGLLGAGFGAAIDPEKEYIS
                     FGNFGWGPELNNPQEGSISRFYYKDGTPVSPSNGFTPGLSRVQGMDYDKSGNLWMASV
                     GCQDPFAPSPTGVYPFKSEPSAVVVYLNGEPSAETMLICDEFPVKQSATSDAVLLRTP
                     YLKIFDVTPDNNGNAYVSCIGNYDEDVADNCVLSAVYKVAVENDKLVVKESWYSDYKS
                     KINKQIGFESLRQVAINGDGDVVVVGVTSSRATILSKDDLTFKGYYEVNTYNPWGVKI
                     DKKETVFLANFGHDTGSVRDNTLDLQAPFGVTMLRDASKPDSAQLLTVPTGGSEVTLA
                     NGHPLYGTQTRPSEPAVLIQGEPTFLQVRMKCYQPIMRLTSTNIDGAGNLWCMNNWKP
                     SALVDVADNPGGDGVVIFLGIAEPE"
BASE COUNT          911 a          949 c          929 g          968 t
ORIGIN      
        1 catcgagaat cgaatgaact acctcgtcgc cgcccgtgcc gaggattgcc gctccaagga
       61 tttcgcgcgt ttcggcgtcg accgcgattt tcataaaacc ttccgtttcg cctttttcga
      121 ccgcgcggct gacgcgtgac atcgggcgtt tgccgattag cgccctgcgt cctgtccggc
      181 gaatttccgc ttcggtcatc ccgacacgcc cgagaggcgg atcgataaac agcccgcagg
      241 cagggaagcg gtcgctagtc cggcgtgctt cattgtcgag caggttagcg gcaacgattt
      301 cggcatcatt ccaggaggta tgcgtaaagg cgtttttccc attgcaatcg ccgagcgccc
      361 agatgccggg cacattcgtg cgcagttcgt cgttaacctt gatgtaaccg cgcgagccgg
      421 tttcgatacc tgcgcgatca agtcctaaac gagctcgtcg gggcaatcgc cggcattgcc
      481 agagtttctc tggcaatgga ttatgaccgg attgagacgc gaaaggcgcg ttctattgtt
      541 cgaaggttcg ccccgagttt taggaacgtt cgccgaagag ttctcaaaaa gcgctgaaca
      601 tcaactgcgt gatttaggtg tcgaagtttt cctgaacagt ttcgttaaac aagtcgaacc
      661 gaaccgcatt aaagtgggcg agcaatggat cgaatgcagc gtcactttgt gggcaacggg
      721 cgttatggct tcagaactcg gcgagcggtc ggctgctaaa actgaccggg cgggcagagt
      781 tctggtcgag ccggatttat cgattccgac ttacaaaaac attttcgtca tcggcgatat
      841 ggcttttttg aaagatgctg gcggcgtcat tgtgccggga ctttctccgg cagcgataca
      901 ggaaggcgaa cacgctgcga aaaatattct aggtgattta attgtcgtcg tggacgcata
      961 agctgtcatt gatttacccg taagctgtcg ctgtggattt tctcgcaata aattttaaat
     1021 atttctctca ataagaatgg tctgctaaaa cacttccatt cgcccgcctg catgcggttg
     1081 cagtatttac tcgggctcgg ctatcccgag aaaaattacc acgccgtctc cgcccggatt
     1141 atcggcgaca tcgaccagag ccgaaggctt ccagttattc atacaccata aatttcccgc
     1201 gccgtcgatg ttcgtcgagg ttaatcgcat aatcggttga taacatttca tacgcacctg
     1261 aagaaaagtc ggttctccct gaataagcac tgccggttct gaaggtctgg tttgcgtccc
     1321 gtaaagcgga tgcccgttgg cgagcgtgac ttccgaaccg cctgtcggaa ctgtaagaag
     1381 ctgcgcggaa tccggctttg acgcatcgcg aagcattgtc acgccgaacg gcgcttgcaa
     1441 atcgagggta ttatcgcgaa cggaacccgt atcgtgtccg aaattggcga ggaaaaccgt
     1501 ttctttctta tcaatcttca ctccccacgg attgtaagta ttgacctcgt aataaccttt
     1561 aaaggttaaa tcgtctttgg agagtatcgt cgcccggctg ctggtaactc cgactaccac
     1621 tacgtctcca tctccgttta tcgccacctg ccggagagat tcgaaaccaa tctgcttatt
     1681 gattttactc ttataatcgc tgtaccagct ctcttttacg accaatttat cgttctcgac
     1741 agctactttg taaaccgccg aaagcacgca attatcagcc acatcttcat catagttgcc
     1801 gatgcaggaa acatacgcgt ttccattatt atcaggagtg acgtcaaaaa tcttcagata
     1861 aggagttctt aaaaggacgg cgtccgatgt tgccgactgt ttcaccggaa actcgtcgca
     1921 aatgagcatc gtctctgccg aaggttctcc gttcaggtac acgactacgg cgctcggttc
     1981 actcttaaac gggtaaacgc ctgtcggcga cggagcaaaa gggtcctgac agccgacgga
     2041 agccatccag agatttccgg atttatcgta gtccattccc tgcacgcgcg acagtccggg
     2101 cgtaaatccg ttggaaggac tgacgggcgt cccgtcttta taataaaacc gggaaatgct
     2161 tccttcctgt ggattattca gctcaggtcc ccagccgaaa ttaccgaaag aaatgtactc
     2221 tttttcaggg tctatcgccg ctccgaagcc tgcgccgagc aatccaccgc cctgcaccgg
     2281 agagaaatca gccggttggg cgttgtattt atacaccagg caatgcgttc cggaaagatt
     2341 cgagccggct ctgaaattgt tcgccaccca cgcattatcg tcggcgtcga agaccacaaa
     2401 agcgagtccg ctcggaataa aattcttcgc gcccgaagaa ttgctcttga gcgtcagcgt
     2461 ccagttgtgc ggggcttttt catcgtcgga aacgttcatc gacgtcaatg aatcctgata
     2521 aatttccggc tttccgacca gcatctgata gatattgtcg gcattggtaa acgggtcgta
     2581 cagcatattt ctgaccgctt gcagaaagtt gttattcggc gcgttttctt cgtgccacgc
     2641 atagtcgagg aactgattgt agaagccggt atcggtcaaa caatagtaca aaaggttgca
     2701 caacgaattg aacatcgggt aactgttcgt ttcaaatccg ttgggcgact cctttatcat
     2761 atccgccaca gttccttcgg tcaggtaaaa gttttgcttc attccgtaag caatcttcat
     2821 attgcgctcg ctgccgctca tcgtgaccag accgtcgctc tgcaccgtcg tcattcgcgc
     2881 aaacgtatag atggaggcga tggacgactc cgggctgacc ttgacgacat agtcggcgtc
     2941 gggcagatac agcgttgcca gcagagttaa gggtttatct tcgacgttat acgaaacgcc
     3001 gttgagcgta taagttttga taaaaaccgt gacggcgaag tattcggatt ggtcttccgg
     3061 cagcgcggcg ctgaacgtat ttcccttaaa gtagctgcta ttgtaagccg agccgacctg
     3121 accgctgaca atgccgcgaa cctgagcagc gcccgaagtg atttcaagac ccttgatttc
     3181 tacgttaaat tgaactgttt ttgcaggcat aagtatgtta taggtttata aagcggcagg
     3241 cttcacttgc attgacagcg gaaaaaacaa cgcgcggcga attgaaatcc ggctgttttc
     3301 cgggtcattg aagagagcat tccgacagtt aaatatccaa aattataatt taaatcgcgg
     3361 taaatggtat cttaaagtcg ccccgttaca aaacctcaaa agccgaaata atcattcatc
     3421 tttgaaattg tcgttaccgc ctcgcctctt ctgtcgtttc acggagattg tcgcggcgag
     3481 tttctcgaac tgacgatttc atcattccac tacatccctg agtgttcaga gaacaggtaa
     3541 agccagagca aactggtttc gatgtttccg gggatggcta tgtggagaat cacggaaact
     3601 actttactcg ccggaaatat cactcgattt ttgaacattc actgcgacag tctaaagcct
     3661 gaaaattttt gacagtttat gggtaaaaaa tggcgatatt gacaacttgt aggtaaattt
     3721 actggcagat tatgagtaaa ctaaagctaa gctgttg
//
LOCUS       DASWNE010001087         5681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_351133, whole genome shotgun sequence.
ACCESSION   DASWNE010001087 DASWNE010000000
VERSION     DASWNE010001087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5681
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351133"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VGB68_20735"
     CDS             complement(<1..657)
                     /locus_tag="VGB68_20735"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY0051737.1"
                     /translation="MSLTVVGSVAFDALETPFGKRDKILGGAGTHFALSASFFTQINV
                     VGVVGNDFTETEMDVFRRHNINTDDIERVSDGKTFFWRGRYDYDLNTAHTLETQLNVF
                     ETFQPKLSESSKNNRLLFLANIVPMIQKDVREQCPNAEFVAMDTMNFWIDSMKDAVLE
                     TVKVVDCVIINDAEARQLADEPNIHKAAKKIMEIGGLRALVIKRGEYGAALYTKENYF
                     A"
     gene            complement(696..1016)
                     /locus_tag="VGB68_20740"
     CDS             complement(696..1016)
                     /locus_tag="VGB68_20740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051738.1"
                     /translation="MWKSGEEAAVESCLYSILEVVNKATKAGKLQIPAKARELTQTEI
                     EQLLENQGLDCKDKSNLSREVHIAVGDVNEHTPLKMKVWTNGDDEIAGTDDDLVVPLG
                     EKVK"
     gene            complement(1103..1483)
                     /locus_tag="VGB68_20745"
     CDS             complement(1103..1483)
                     /locus_tag="VGB68_20745"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY0051739.1"
                     /translation="MNLNQVTVYTNKPFESIEFYKKLGLRLIVDSAPRYARFECPDGS
                     STFSLHETGKIDSGNSNIVLYFECENLDAKVAELKKLGLEFEQEPVDQTWLWREAYLR
                     SPEGNRICLFYAGENRQNPPWRVK"
     gene            complement(1561..2019)
                     /gene="bamD"
                     /locus_tag="VGB68_20750"
     CDS             complement(1561..2019)
                     /gene="bamD"
                     /locus_tag="VGB68_20750"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEY0051740.1"
                     /translation="MKKAFLFLSFIACLFVFSENVFAQKQIEPKEVRDPILEADAMHN
                     LEVARQAFRLRKAYKGVLMRCEEIIAANPIFSKMDEVLYLSGMSSYYLSLNKGKQKIN
                     PNFQQEKEKYAPEKLREDAAAYLKQLTETFPQSEFRADAEKTLKLLEAKK"
     gene            complement(2166..3056)
                     /locus_tag="VGB68_20755"
     CDS             complement(2166..3056)
                     /locus_tag="VGB68_20755"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF005657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEY0051741.1"
                     /translation="MEQVKIGIIGGSGLYQMPELTDIKEIEVETPFGKPSDAFIIGTL
                     EGERVAFLPRHGRGHKLTPTELPFRANIYAMKLLGVDYILSVSAVGSLQEQYAPTDMV
                     IPDQFFDRTRARAEESTFFGAGIVGHITFAHPVCDELGGILEESCKAVEGLKVHRGGT
                     YLCMEGPAFSTKAESNVYRSWGMDIIGMTNLQEAKLAREAEIAYATLALVTDYDCWHE
                     GHDDVSVDMVIEYLHKNVRNAQLVLKEAVRRVAAKETPNPFEGATKNAIFTAPELWNP
                     ETAKRLEAIIGKYATKAANK"
     gene            complement(3069..3542)
                     /locus_tag="VGB68_20760"
     CDS             complement(3069..3542)
                     /locus_tag="VGB68_20760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051742.1"
                     /translation="MKKLLLLIILAAVTAQFVGGQSLENQNEKDKFIAAVQSLEDAPA
                     GETAKETREWALEYSQQVKYPFCDEISFLFFAPEIRGEVMSQYLMKLAAFELENPGRK
                     SEANAAQAAGLESALKVYEKIVKAVPKAKNRKLDSLVELRNKNQLMNFVKSANCK"
     gene            complement(3558..4394)
                     /locus_tag="VGB68_20765"
     CDS             complement(3558..4394)
                     /locus_tag="VGB68_20765"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY0051743.1"
                     /translation="MRLGDYRIEIIPDTEFRLDGGAMFGVVPRVVWERVSPPDDANRI
                     QLNMNCLFIETPSEKILVETGIGEKWTEKQTQLYGIFRAKSFAQALFGKTGYMPEDIT
                     IVVNTHLHFDHAGGNTKSDRNGKAFPQFPNARYFVSKSEFEHAENPHERDRASYLKEN
                     WQPVLESGQLELKPDVYEVVEGLTIENARGHSETMQTVCLKRGGETLYGFADLIPTRH
                     HLALPWIMGYDLYPTETLEFKKRVLPRAVKENWICLFYHDFEMPLCRLTTEDGKIKPR
                     SI"
     gene            complement(4516..>5681)
                     /locus_tag="VGB68_20770"
     CDS             complement(4516..>5681)
                     /locus_tag="VGB68_20770"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY0051744.1"
                     /translation="ESLKQTLAMASQARKDGMANEPEVKEELESIRTEIVAASYDKKK
                     NADKGQMPPFGFISEDEVKAFYGEAGNEAKFKTFIDKKIEQAKEDGRIPKDREPTEEQ
                     LASAKDFYAKARIYEKEAESKKGELGEEYNKKVELQVKLQQEQFLARRYADKVMKDKV
                     KVTDADIQEYLKSHPELDTKKDKRAKAEEILARAQKGEDFSALAKEFSEDPGSKEKGG
                     LYENVRKGQFVPEFETAALGLEAGQIAPQLVETKYGFHIIKLEKKGTAKDPAGGEEAP
                     TFDARHILISTMITDPENPRVPPMSPEDKVRAQLEEEKEKKIMDELLAKNPIEIPDDF
                     VVPEPSAEQLEQMKKMQEMQMQQQMQQMQQPPEGDETDAPKGKTDKKPEPKKK"
BASE COUNT         1216 a         1474 c         1332 g         1659 t
ORIGIN      
        1 cgcaaaataa ttttcttttg tgtaaagcgc cgccccgtat tcgccgcgct tgatgaccag
       61 cgcgcgtaaa ccgccgattt ccataatctt tttcgcggct ttgtgaatat tcggctcgtc
      121 cgccagctgg cgcgcctcgg cgtcgttgat gatgacgcaa tcgacgacct tgaccgtttc
      181 cagaacggcg tctttcatcg aatcaatcca gaaattcatc gtgtccatcg ccacaaactc
      241 ggcgttcggg cattgctcgc ggacgtcttt ctgaatcatc ggaacgatgt tcgccagaaa
      301 taaaagacga ttgtttttcg agctttcgga caatttcggc tggaaagtct cgaaaacgtt
      361 aagctgcgtc tccaaagtgt gagccgtgtt caaatcgtaa tcgtaacgcc cgcgccagaa
      421 aaaagttttt ccgtccgaga cgcgctcgat gtcgtccgtg ttgatgttgt gacgccggaa
      481 aacgtccatt tccgtctcgg taaaatcgtt gccgacgacg ccgacgacat taatctgcgt
      541 aaaaaagctg gctgaaagcg caaagtgcgt gcccgcgccg cccagaattt tgtcgcgctt
      601 gccgaacgga gtttccaaag cgtcaaacgc caccgaacct acaactgtta aagacattta
      661 ttaaaaaatt ctcctcaata atttcaaatt taaagttatt ttactttttc gcccaacgga
      721 actaccaaat catcgtccgt tcccgcaatt tcatcgtcgc cgtttgtcca gactttcatt
      781 tttaacggag tgtgttcatt gacatcgccg acggcaatgt gaacttcgcg tgataaatta
      841 cttttgtctt tgcaatccaa gccttgattc tctaaaagct gttcgatctc ggtttgagtt
      901 aattctctcg ctttagcggg aatttgtaat ttaccggctt tggttgcttt attgacaact
      961 tctaaaattg aatacagaca agattctacg gcggcttctt caccgctttt ccacatttta
     1021 tatgcccagc gtccggcgac gaacaacagc aaagcaagta ataaaattgc aattttgttt
     1081 tggatgcttc tggtcaacta aatcatttta cacgccacgg cggattttgg cgattttcgc
     1141 ccgcgtagaa aagacaaatt cgatttcctt cgggcgagcg cagataagcc tcgcgccaga
     1201 gccacgtctg gtcgacgggt tcctgttcaa actccaagcc gagttttttc agttccgcaa
     1261 ctttcgcgtc caggttctcg cattcgaaat aaagcacgat gtttgaattg cctgaatcga
     1321 tcttgcccgt ttcgtgaagc gaaaaggtcg agctgccgtc gggacactcg aagcgcgcgt
     1381 agcgcggcgc ggaatcgacg attaagcgaa gtccgagctt tttataaaat tcgattgact
     1441 caaagggctt gttcgtgtaa accgtgactt gattcaggtt catcgagaga tttagtttag
     1501 cggcgagatg accggcgaaa aaaaatattt ccgggtaagc tcgccgctaa ataattgcgc
     1561 ttattttttc gcttcgagca atttcagggt cttttccgca tccgctcgaa attcgctctg
     1621 cgggaaggtt tccgtcagtt gtttcagata cgcggcggca tcttcgcgca gtttttcggg
     1681 cgcgtatttt tccttttcct gttggaaatt cgggttgatt ttctgcttgc ctttattcag
     1741 cgaaagataa tagctgctca ttcccgaaag atataaaacc tcgtccatct tggaaaaaat
     1801 cgggttggcg gcgatgattt cctcgcagcg catcagcacg cctttgtaag ctttgcgcag
     1861 gcgaaaagcc tgtcgcgcga cttccaggtt gtgcatcgcg tcggcttcca gaatcgggtc
     1921 gcgaacttct ttcggctcaa tctgcttttg cgcgaaaaca ttctcggaaa acacaaaaag
     1981 acacgcgata aaactcaaaa ataaaaatgc ttttttcata aattgcaaaa ctcctgtccg
     2041 gtttgatgcg gcgaaattat ttgaagtttg atgccggtaa agaaaacgat aaatgatgaa
     2101 cgatgaattg cagttttcac tcggcgtctg tttcattcat cgttcatcgt tttcgcgttc
     2161 atcgtttatt tattcgccgc tttcgtcgcg tatttgccga taatcgcttc cagccgtttc
     2221 gccgtctccg gattccaaag ctcgggcgcg gtgaaaatcg cgtttttggt cgcgccttca
     2281 aacgggttcg gcgtctcttt cgccgcgacg cgccggacgg cttcctttaa aacgagctgc
     2341 gcgttgcgca cgtttttgtg caggtattcg atgaccatat cgaccgaaac gtcgtcgtgt
     2401 ccttcgtgcc agcaatcgta atcggtgacg agcgcgagcg tcgcgtaggc gatttcggct
     2461 tcgcgggcga gcttggcttc ctgcaaattc gtcattccga taatgtccat tccccacgag
     2521 cgataaacgt tcgattccgc cttggtcgaa aaagcgggac cttccataca cagataagtt
     2581 ccgccgcgat gaacttttaa gccttcgacc gctttacacg attcttccag aatgccgccc
     2641 agctcgtcgc agaccggatg cgcgaaagtg atgtgcccga ctatccctgc gccgaaaaac
     2701 gtcgattctt cggcgcgcgc gcgcgtgcgg tcgaaaaact ggtcgggaat gaccatatcg
     2761 gtcggcgcgt attgttcctg cagagagccg accgccgaaa ccgacagaat atagtcgacg
     2821 ccgagaagct tcatcgcgta gatattggcg cgaaagggaa gttccgtcgg cgtcagttta
     2881 tgcccgcgcc cgtgacgcgg cagaaacgcg acgcgctcgc cttccagcgt gccgataata
     2941 aaagcgtcgg acggcttgcc gaaaggcgtc tcgacttcga tttccttaat atccgtcaat
     3001 tccggcatct ggtataaacc gctgccgccg ataattccga tttttacttg ttccatttaa
     3061 ttattttact atttacaatt tgcagatttt acaaagttca ttaattgatt tttattccga
     3121 agctccacca aactatcaag tttcctgttt tttgctttcg ggacggcttt cacgattttt
     3181 tcataaacct ttagagcgct ttctaaacca gccgcctgag ccgcgttcgc ctcggatttt
     3241 cttcccgggt tttccagttc aaaagcagcc agtttcatca aatattgaga cataacctcg
     3301 cctcttatct cgggagcaaa aaatagaaac gaaatttcgt cgcaaaatgg atatttcacc
     3361 tgctgcgaat actccaaagc ccactcgcgg gtttcttttg ccgtttcgcc ggcgggagca
     3421 tcttcgagag attgaaccgc cgcaatgaat ttatcttttt cattttgatt ttctaagctt
     3481 tgaccgccga caaattgcgc tgttacggcg gctaaaatta ttaatagcag aagttttttc
     3541 attttgcgat ttccctgtta aatcgagcgg ggctttattt tgccgtcttc cgtcgttaga
     3601 cggcaaagcg gcatttcaaa atcgtggtag aaaagacaaa tccaattttc tttcacggcg
     3661 cgcggcagaa ctcgcttctt aaattccaat gtttcggtcg ggtacaaatc gtagcccata
     3721 atccacggca aagccagatg atgacgcgtc ggaatcaaat ccgcaaagcc gtagagcgtt
     3781 tcgccgccgc gctttaaaca aacggtctgc atcgtctcgg aatgcccgcg cgcgttttct
     3841 atcgtcaagc cttcgacgac ctcgtaaacg tccggcttta attcaagctg accgctttct
     3901 aaaaccggct gccaattttc cttcaggtaa cttgcgcggt ctcgctcgtg cggattttcg
     3961 gcgtgctcga attcgctttt tgaaacgaaa taccgggcgt ttggaaattg cggaaacgct
     4021 ttcccgttcc tgtctgactt tgtgttgccg cccgcgtggt cgaaatgcag atgcgtgttg
     4081 acgacgattg taatatcttc cggcatatat ccggttttcc cgaaaagagc ctgggcaaaa
     4141 gatttcgcgc ggaaaatgcc gtaaagctgc gtctgttttt ccgtccattt ttcgccgatg
     4201 ccggtttcta caagaatttt ttcgctcggc gtttcgatga acaggcagtt catattcaat
     4261 tgaattcgat tcgcgtcgtc cggcggcgag acgcgctccc aaacgacgcg cgggacgact
     4321 ccaaacatcg ccccgccgtc cagtctgaat tccgtatcgg gaattatttc gattcggtaa
     4381 tcgcctaacc gcatagtttg cagaaagctt atagatagtt gcagaaagct tcagatagtt
     4441 gccgatagat taaaactcag aaactgtttt taacttttcg caactatctg ccaacttccc
     4501 gcaactatct gtcaactatt tctttttcgg ttcaggcttt ttatccgtct tgcctttcgg
     4561 cgcgtcggtt tcatcgcctt cgggcggttg ctgcatttgc tgcatttgct gctgcatctg
     4621 catctcctgc attttcttca tctgttcgag ctgttcggcg gaaggttcgg ggacgacgaa
     4681 atcgtcggga atctcaatcg gatttttcgc cagaagctcg tccattatct ttttctcttt
     4741 ttcctcttcg agctgcgcgc gcactttatc ttcgggagac atcggcggca cgcgcggatt
     4801 ttccgggtcg gtaatcatcg tcgaaatcag gatgtggcgc gcgtcgaacg tcggcgcttc
     4861 ctcgccgccg gctgggtctt ttgccgtgcc tttcttttcg agcttgatga tgtgaaaacc
     4921 gtatttggtc tcgactaatt gcggcgcgat ttgtccggct tccaatccga gcgcggcggt
     4981 ttcaaattcg ggaacgaatt gacctttgcg gacgttttcg tagagcccgc ctttttcctt
     5041 gctgcccggg tcttcggaaa attctttcgc cagcgccgaa aaatcttcgc ctttttgagc
     5101 gcgcgccaga atttcttcgg ctttggctct tttatccttt ttggtgtcga gctccgggtg
     5161 cgacttcaaa tattcctgaa tgtccgcgtc cgtcaccttc accttgtctt tcataacttt
     5221 atcggcgtag cggcgcgcca gaaattgctc ctgctgaagc ttgacctgaa gctcgacttt
     5281 tttgttgtat tcttcgccga gctcgccttt tttcgattcg gcttcctttt cgtagattct
     5341 ggctttggcg taaaaatctt tcgccgaggc gagctgctct tcggtcggct cgcggtcttt
     5401 cggaatgcga ccgtcttctt tagcctgctc gattttcttg tcgataaagg ttttaaactt
     5461 cgcttcgttg ccggcttcgc cgtaaaaggc ttttacttcg tcctcgctga taaaaccgaa
     5521 aggcggcatc tgacccttgt cggcgttttt ctttttatcg taactggcgg cgacgatttc
     5581 cgtgcggatg gattcgagtt cttctttgac ttccggctcg tttgccatac cgtccttgcg
     5641 cgcctggctc gccattgcca gcgtctgttt caggctttcg a
//
LOCUS       DASWNE010001088         4079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_635649, whole genome shotgun sequence.
ACCESSION   DASWNE010001088 DASWNE010000000
VERSION     DASWNE010001088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635649"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..718)
                     /locus_tag="VGB68_20775"
     CDS             complement(161..718)
                     /locus_tag="VGB68_20775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY0051745.1"
                     /translation="MTDKSDVTQLLNKAQSGDKESLDKLLPLIYDELRRVAANQLRAE
                     RENHTLQATALVHEAYLRLLEQHEVDWQNRAHFFSIAAEMMRRILVNYAIQRNAKKRG
                     DGVTRISLDDAISFSNGEADLDLVSLDASLKRLAEFDEMQARIVELRFFGGLTIEEVA
                     EILAISESTVKREWKMAKAWLKTQL"
     gene            1087..2091
                     /gene="recA"
                     /locus_tag="VGB68_20780"
     CDS             1087..2091
                     /gene="recA"
                     /locus_tag="VGB68_20780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEY0051746.1"
                     /translation="MSMDKSKAVEAALANIEKKFGKGSIMRMGERVSEQVGSISTNCL
                     SLDAAIGVGGFPRGRIIEVYGPESSGKTTLALQVVASAQRMGGIAAYIDAEHAMDPEY
                     ATKLGVDIDDLLISQPDSGEQALEIAETLVRSNSVDVIVIDSVAALVPRAELDGEMGD
                     SLPGLQARLMSQALRKLTAIVAQSNTCFIFINQLREKIGVMFGSPETTTGGKALKFYA
                     SLRLDIRRIGAIKEGDRHVGNRTKVKMVKNKVAPPFRECEFDIMYGEGISKEGDMLDL
                     AVTNNIVEKSGAWFSYKGERLGQGRENVKNSLKENIELRDRIEYDVKLALGLIEKEAE
                     "
     gene            complement(2178..2510)
                     /locus_tag="VGB68_20785"
     CDS             complement(2178..2510)
                     /locus_tag="VGB68_20785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051747.1"
                     /translation="MTNQTLPAGITISPMPVSGNTPGIPANAAVVNMNALPKGSTPIP
                     GIDPKKANQPLKPGGTPTPGIPDPKTMQKQNVPPMENPNSSQSDSPNKPASNKVDSVN
                     KPQTVRKP"
     gene            2503..2646
                     /locus_tag="VGB68_20790"
     CDS             2503..2646
                     /locus_tag="VGB68_20790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051748.1"
                     /translation="MVTAELAPLELAAAFGSLADPQARLNPKIEQNKTNKNFFNIKKI
                     LAD"
     gene            2942..3017
                     /locus_tag="VGB68_20795"
     tRNA            2942..3017
                     /locus_tag="VGB68_20795"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2975..2977,aa:Phe,seq:gaa)
     gene            3079..3381
                     /locus_tag="VGB68_20800"
     CDS             3079..3381
                     /locus_tag="VGB68_20800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051749.1"
                     /translation="MSKGLKVILFASQTPLFETKLELVNKAELKTKLDSPGINENFYS
                     LDGQLLPGRLVLYHSYENWNVFYFDERGNRNDEVTFQSENEACQYIYEVIGKRELK"
     gene            3476..3820
                     /locus_tag="VGB68_20805"
     CDS             3476..3820
                     /locus_tag="VGB68_20805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051750.1"
                     /translation="MAEWHLNELRNELEKKGWRIVAEHPSRHLYVSGSWEIQRNPSMP
                     PIFIDFEGIDDLNTLPMNESYACNIRNHKADSLYFSKRGEKSDSNRRTIWKGNLVNFV
                     KQLDLLSEQVNK"
     gene            3866..>4079
                     /locus_tag="VGB68_20810"
     CDS             3866..>4079
                     /locus_tag="VGB68_20810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051751.1"
                     /translation="MPDKHKPYPRNIEGDFYVADGCCMTCMVTEVCAPDLMGFDETEN
                     HCFVAKQPANENEVYQAIKATWSAEFD"
BASE COUNT         1121 a          882 c         1013 g         1063 t
ORIGIN      
        1 cctctcggcg acggtcattt ccgccgcccg gttgaagatt tccttgattt tttgataacg
       61 ctcggcgttc ataaaattca ggattcagga ttcaggattc aagattcagg attcgggatt
      121 taggattaca aatcttgaat cttgaatctt gaatcctgaa ttagagctgc gttttgagcc
      181 aggctttcgc cattttccat tcgcgtttga cggtcgattc ggaaatggcg agaatttcgg
      241 cgacttcttc gatggtcaag ccgccgaaaa acctgagctc gacaatgcgc gcctgcattt
      301 catcaaattc cgccaggcgt tttaaggaag cgtcgagcga aaccaaatcc aaatcggctt
      361 cgccgtttga aaagcttatc gcatcgtcga gcgaaatgcg cgtgacgccg tcgccgcgtt
      421 ttttggcgtt gcgctggatg gcgtaattga ccagaatccg ccgcatcatc tcggctgcaa
      481 tcgagaaaaa atgcgcgcga ttctgccagt caacctcgtg ttgttcgagc agccgcaggt
      541 aagcttcgtg aacgagcgcc gtcgcctgca aggtgtgatt ttcgcgctcg gcgcgcagtt
      601 gatttgccgc gacccgccgc agctcgtcgt aaatcaaagg cagaagcttg tcgagcgatt
      661 ccttatcgcc gctttgcgct ttattcagaa gctgagttac gtcggattta tcggtcattt
      721 aaaaagcttg gattcgagag tttactaatg gttgtcccgt cagacattct acaagaaaat
      781 ttttaaaaac ctaattatct ccggaacctc cgagctgttt tcgcaaattt ccgcgattta
      841 aaccgtaaga aaaagttttt tgtctgatgc gataacgaaa cttgcagaag ttttcgtttt
      901 ccactaactt agaaaagctt ggcggtaaaa atttctcaaa aacgaaagaa atttttgttt
      961 catacgtgaa acaacatttc acaaaaacat ctttttgtgt tactcttata acagtctgac
     1021 aacaaagctg gacgatagaa gtaatacaaa aggagaaaga aaacattggc taaagaggca
     1081 gtggcaatga gcatggacaa aagcaaggct gtcgaagcag ctctggcaaa tattgaaaag
     1141 aaattcggca aaggctcgat tatgcggatg ggcgagcgcg tgtccgagca ggtcggctca
     1201 atctcgacaa attgtttgag cctggacgcg gcgatcggcg tcggcggttt tccgcgcggt
     1261 cgcattatcg aagtttacgg accggaaagc tccggcaaaa cgacgctcgc cttgcaggtc
     1321 gtggcgtcgg cgcaaagaat gggcggaatc gccgcttaca tcgacgcgga acacgcgatg
     1381 gacccggaat acgcgacgaa actcggcgtc gatatcgacg atttgttgat ttcgcagccg
     1441 gattcgggcg agcaggcgct ggaaatcgcc gaaacgctcg tgcgctcgaa cagcgtcgac
     1501 gttatcgtta tcgattcggt cgccgcgctg gttccgcgcg cggagctgga cggcgagatg
     1561 ggcgattcgc tgcccggact tcaggcgcgc cttatgtcgc aggctttaag aaagctgacg
     1621 gcaattgtcg cgcagtcgaa tacgtgtttt attttcatca accagctgcg cgaaaaaatc
     1681 ggcgttatgt tcggctcgcc cgagacgacg accggcggca aggctctgaa attttacgct
     1741 tcgcttcgtc ttgacattcg ccgcatcggc gcgatcaagg aaggcgaccg tcacgtcggc
     1801 aatcgcacga aagtcaagat ggtgaaaaac aaggttgcgc cgccgttccg cgaatgcgaa
     1861 ttcgacataa tgtacggcga aggcatctcg aaagaaggcg atatgctgga tttggcggtg
     1921 actaacaaca tcgtcgaaaa aagcggcgcg tggttttcct ataaaggcga acggctcgga
     1981 caaggacgcg aaaacgtcaa aaattcgctc aaggaaaata tcgagctccg cgaccgcatc
     2041 gagtacgatg taaaactcgc gctcggttta atcgaaaaag aagctgaata gttttatttg
     2101 aattcaggaa actaaaaagg cgagagtgtt ttttataaaa aacactctcg cctttttgct
     2161 gtaaattaaa aattcgctta cggttttctg accgtctgcg gtttatttac cgagtcgacc
     2221 ttgtttgacg ccggtttgtt gggcgaatcc gattgcgacg aattcggatt ttccatcggc
     2281 ggaacgtttt gtttctgcat agtcttcggg tcgggaatgc cgggcgtcgg cgtcccgccg
     2341 ggcttcagcg gctggttcgc ctttttcggg tcgattcccg gaatcggcgt cgagcctttg
     2401 ggcagggcgt tcatattaac gacggcggca ttagccggaa tgccgggcgt gtttcccgaa
     2461 accggcatcg ggctgatcgt aattcctgcc ggcagcgtct gattggtcac cgctgaattg
     2521 gcaccgttag aattagccgc tgcattcggc tcgcttgccg acccgcaagc caggttaaat
     2581 cctaaaatcg aacaaaacaa gacgaataaa aatttcttca acatcaaaaa aatcctcgct
     2641 gactaaaaat aaaaatagct gtgccggctt gaatgtagcg tctccggggg acggggacgg
     2701 cgttcaaacc ggcacgggca ggtatcaaac ggacttcgga aggtatattt gtagtagctg
     2761 aaacataggc aaacaactac taaattgtga ttatattccg attggtcgaa ttgtcaatac
     2821 taaatttaat aataagccgt attttcgcct gcttttgcgg attttcagta gggatttgac
     2881 aagtcgaaac ggcttcttta cactaattta cttgcttcga taaattattt atcgagcaac
     2941 cggcttggta gctcagttgg tagagcagcg gactgaaaat ccgcgtgtcg gcagttcgat
     3001 tctgtcccaa gccaccatta ttttctatag tttagggcta ctttacagta gcccttttcg
     3061 ttttggcggg gcgttggtgt gtcaaaaggt ttaaaggtga tattatttgc ctcacaaacg
     3121 cccctttttg aaacgaaatt agaattagtg aataaagcag aactaaagac gaaattagat
     3181 tcaccaggaa taaacgagaa cttttattct ttagatggac aactgcttcc cggtagatta
     3241 gtgctttatc attcttacga gaattggaat gttttctatt ttgacgagcg aggaaataga
     3301 aatgatgaag ttacctttca atctgaaaat gaagcctgcc agtatattta cgaagttatt
     3361 ggcaaaagag agttaaaata aacagtcccg aaaacactgc tttttgagac aattaaaata
     3421 gactaaaatt tgtttcaaaa acgacctttt cgagacaaaa tcaacataat tcaatatggc
     3481 tgaatggcat ttgaatgaat tacggaatga gctggagaaa aaaggctggc gcattgtagc
     3541 agaacatccg agccgacatt tatatgtttc gggttcctgg gaaattcaac gtaatccttc
     3601 gatgcctccg attttcattg atttcgaagg aatagatgat ttgaacactt tgccgatgaa
     3661 tgaaagttat gcgtgcaata tccgtaatca taaagcggac agcttatatt tcagtaaacg
     3721 tggtgaaaaa tcagattcaa acagaagaac aatctggaaa ggcaatctag taaattttgt
     3781 aaagcagttg gacctattga gcgaacaagt taataaataa agagtctcga aaacgaccct
     3841 tttagggaca ttaaaaacaa tagctatgcc cgataaacat aagccatatc ccaggaacat
     3901 tgaaggagat ttttacgttg cagacggttg ctgtatgact tgtatggtga ctgaggtttg
     3961 cgctcccgat ttaatgggtt ttgatgaaac cgaaaatcac tgttttgttg cgaagcagcc
     4021 ggctaatgaa aatgaagttt atcaggcaat taaagcgacc tggtcagccg agttcgatt
//
LOCUS       DASWNE010001089         4557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_635653, whole genome shotgun sequence.
ACCESSION   DASWNE010001089 DASWNE010000000
VERSION     DASWNE010001089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4557
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635653"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VGB68_20815"
     CDS             complement(<1..257)
                     /locus_tag="VGB68_20815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051752.1"
                     /translation="MKIVENSIDYTNYTAAEINTMAKICCQKARRFEDAGSYATAESV
                     MSPFWSGVGTRPHTERLEEEIKAEVLLRCGVIAGWIGNNKS"
     gene            complement(365..1462)
                     /locus_tag="VGB68_20820"
     CDS             complement(365..1462)
                     /locus_tag="VGB68_20820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051753.1"
                     /translation="MNLKLNAVAPEANSPIKRIIFLFLALFLLNGFSALNIPAESGKI
                     AYASANAIYKMNADGTSVQQLTFTGSNQRDFSPVWSPNGTKIAFVRSTATQEETTGGG
                     SGGDGDGDWEWIPSDPTYYTVYSYSLYTMDYNGANLTLIRNSPTFINDLTWSPDGTKL
                     AYVQGADTTYMGVFQTCGGNSNIYIVDAVAGGAASQVTAAVNGIDPSWSRDGTKIFYA
                     VNNSAENFGIYSVNLSDNSVQRWTYDGAAPADPEISPDGSKIVYATGYAEQECLFGNM
                     STMGPTRMYTYRGSLILYDIAQNSYQMLTSTASSPTWNPTGTVILYVNSGGSYGAEGD
                     TGQELTTITATGYNQMRIQNVQTEESGSWSH"
     gene            1814..3058
                     /locus_tag="VGB68_20825"
     CDS             1814..3058
                     /locus_tag="VGB68_20825"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY0051754.1"
                     /translation="MKNFAGLLSRESAIMKIVLTFVLMILLSQFVSAQNTYTTYANLP
                     YADDGNVRHKLDLYVPDGATSSVPVIVWIHGGGWQSGDKQLGPNSHPLRYARNGYAVA
                     SINYRLSDEAIFPAQIHDVKAAIRWLRANAAQYNLDATRFGAWGQSAGGHLASLVGTS
                     GDVKTLEGTVGGNTQFSSRVQAVVDWYGPTDFLRMDAQLTQNGCPNPNHNSPDSAESR
                     LMGCAIQTCPDAVRRANPMTYVTSDDPPFFIEHGTADCTVAPGQSRIFQNLFQSVGHD
                     SSLTFLQGAGHGGPQFTAESNLLLVDAFWNAKLRQASNPLINSIKVYRKNLEVNHFRP
                     GALGSLYRISVSGANFQADTKVIINGIEKGVSFVDGNEIVIYGFTGRIPSEGEMNIQL
                     RNSNGRFSNVSRTEIRELNLSN"
     gene            complement(3273..3875)
                     /locus_tag="VGB68_20830"
     CDS             complement(3273..3875)
                     /locus_tag="VGB68_20830"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY0051755.1"
                     /translation="MSGERVYAGRLDDGVGEMRLAQEYDPLSAVNNGALCNLLVFKRK
                     YDEAGKFCRRAVELAPELLEVHISLGESYLFTGRYADAADEFNRALETGKDDAYALAS
                     LGYLYAVTQRSSEAEKIVEKLMSLIPEQPSLYVEAALIDYTLGKKDKAFSMFYKAFEM
                     KVMSGTSLRYSPRFDKIKTEPGYVELLKRHQLSGLTETDF"
     gene            4222..>4557
                     /locus_tag="VGB68_20835"
     CDS             4222..>4557
                     /locus_tag="VGB68_20835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051756.1"
                     /translation="MNHKKINDANIQPADNACVTGTGTCPPKTGIAAQCHPYNPDDFA
                     TGAANSKAVQVKYADGSARWFMAFNSMIKNSTETPYSAADIWRVLWATSPDGANWTVH
                     PNILFRTIGE"
BASE COUNT         1099 a         1144 c         1152 g         1162 t
ORIGIN      
        1 atcgatttgt tgtttccaat ccagccggcg atgacgccgc accgcagcag cacttccgcc
       61 tttatttctt cttccaggcg ttcggtatgc gggcgcgtcc cgacgccgct ccagaacgga
      121 ctcattacgc tttcagcggt cgcatatgaa ccggcatctt caaagcggcg ggctttttga
      181 caacatattt tcgccatcgt gtttatctcg gcggctgtgt aatttgtgta atcaatactg
      241 ttttcgacaa tcttcattgt tataatgtta agttttcccg acgtttgaag caaaaaagga
      301 gaaagggaaa tttaaaaaaa ccttttctcc tcaataaaaa tcaagtttat aaatcaaact
      361 ttaatcaatg actccacgaa ccggattctt cggtctgcac gttttgaatg cgcatctggt
      421 tgtatccggt cgccgtaatg gtcgttaatt cctgcccggt atcgccctcg gcgccgtaac
      481 ttccgccgct gttgacgtag agaatcacgg ttccggtcgg gttccaggtc gggctgctcg
      541 cggtgctggt cagcatctgg tagctattct gcgcgatgtc atacagaatc aggctgcctc
      601 tgtaagtata cattctggtc ggacccatcg tgctcatatt accgaagagg cattcctgct
      661 ccgcataacc cgtggcgtaa actatttttg agccgtcggg cgaaatttcc gggtcggcgg
      721 gcgcggctcc gtcgtaagtc cagcgctgaa ccgaattatc cgacagattg accgagtaaa
      781 ttccgaaatt ttccgcgctg ttattgaccg cataaaatat tttcgtgccg tcgcgcgacc
      841 aggacgggtc gatgccgttg accgccgccg taacctgact ggcagcgccg ccggcgaccg
      901 catcgacaat ataaatattg ctgtttcccc cgcaggtctg gaaaactccc atataggtcg
      961 tgtccgcgcc ctgcacgtaa gcgagttttg tgccgtccgg cgaccaggtc aggtcattga
     1021 tgaaagtcgg gctgtttctt atgagagtaa gattcgcgcc gttataatcc atagtgtaaa
     1081 gactgtatga atagacggtg tagtaagtcg ggtcgctggg aatccattcc caatctccgt
     1141 cgccgtcgcc gccgctgccg ccgcccgttg tttcctcctg agtagcggtc gagcggacaa
     1201 aagctatctt tgtgccgttc ggcgaccaga ccgggctaaa gtccctctga ttggagccag
     1261 taaaggttaa ttgctgaacg gacgtgccgt cagcgttcat tttgtagatt gcattagcgg
     1321 atgcgtaggc aatttttccg ctttcagccg gtatatttaa ggctgaaaat ccgtttaaga
     1381 gaaacagcgc cagaaagaga aagattattc tcttgatggg ggagtttgct tccggcgcga
     1441 ccgcgtttaa tttaaggttc attttctacc ttcccgtttc aaagaaatta ctgcgattcg
     1501 aattatatat attttctgtg atatgtcaat ataaattaaa gataatacgt cgtttatttt
     1561 ttatacaaaa aatttgttta attacgaatc gtatatagcg gcattcataa cccgaggtat
     1621 aatccgcaat aaaagatgaa taaaataatc gtaaattatt gctttcagat agaaaacctc
     1681 acacgaaaca cgccttcgtt ttttgaacag gcggataaat aacatttttt aagactttct
     1741 cgttattgac ggcgtcaaaa cacaaaacgg tttccagact gtctaacatc gttcgctgca
     1801 aaggaaattt acaatgaaga attttgccgg attattgtca cgtgaaagcg cgattatgaa
     1861 aattgtgctg acgtttgttt tgatgatttt attgtcgcag tttgtttcgg cgcagaatac
     1921 atacacgact tatgctaatt tgccgtatgc ggatgacggc aatgtgcggc ataaattaga
     1981 tttatatgtt ccggacgggg cgacttcgtc cgtgccggta atcgtctgga ttcacggcgg
     2041 cggctggcaa agcggcgata aacagttggg tccgaacagt catccgctgc gttatgcgcg
     2101 aaacggctac gcggtggcga gcatcaatta tcgtctgagc gacgaggcga ttttccccgc
     2161 gcagattcac gatgtgaaag ccgcgattcg gtggctcagg gcgaacgcgg cgcaatataa
     2221 tctcgacgcg acaaggttcg gcgcgtgggg gcagtcggct ggcggacatc tggcttcgct
     2281 ggtcggaacc tccggcgacg tgaagactct ggaaggcacg gtcggcggta atacgcagtt
     2341 ttcaagccgc gtgcaggcgg tcgtcgattg gtacggtccg acggattttc tgcggatgga
     2401 tgcgcaatta acgcaaaacg gctgcccgaa ccctaaccat aattcgccgg attcagccga
     2461 atcgcgcctg atgggctgcg ccatacaaac ctgccccgat gcggttcgaa gggctaaccc
     2521 gatgacttat gtaacgagtg acgatccgcc gttttttatc gagcacggga cggctgactg
     2581 caccgtcgcg cccgggcaaa gccggatttt ccagaatctt tttcaaagcg tcggacacga
     2641 ttcgagcttg acctttttgc agggcgccgg tcacggcgga ccgcaattta cagccgaatc
     2701 gaatctcctg ctggtcgacg ctttctggaa cgctaaactg agacaggcgt ctaacccgtt
     2761 gataaattca ataaaagttt atcgaaagaa tttggaagtc aatcattttc gtccgggcgc
     2821 tctcggttcg ctttaccgaa tatcggtctc cggggctaac tttcaggcgg ataccaaagt
     2881 aataataaac ggaatcgaga aaggcgtttc gttcgtcgac ggaaacgaaa tcgtcattta
     2941 tggttttacg ggaagaattc cttccgaggg agaaatgaac atccagcttc gcaactcaaa
     3001 cggaaggttt tcaaatgttt cgaggactga aatccgcgaa ctgaatcttt caaactgacc
     3061 tgctgccggc aatcatagaa aatcagaaaa actgtcgctt taatcattcg ctgccggcgc
     3121 gataaaccat cctttgtcca aaatacgagc taataatatt ctaatttcag acgcttggaa
     3181 ttttaagcag acgcgccctg cgcttcacaa tccggtctat aaacttatgg ctcttttgtc
     3241 tgtcgtctgc cgcttcatct tccccggaaa gtttaaaagt cggtttccgt taaacctgaa
     3301 agctggtggc gctttaataa ttcgacgtat cccggctctg ttttgatttt atcgaatctc
     3361 ggagagtagc gcagagaagt gcccgacatt accttcattt caaaagcctt gtagaacatc
     3421 gaaaacgctt tgtccttttt tccgagcgtg taatcgatta aagccgcctc gacgtataat
     3481 gaaggctgtt cgggaattaa agacataagt ttttcgacta ttttttcagc ctcggaagaa
     3541 cgctgcgtaa cggcgtataa atagccgagc gaggcgagcg cgtaagcatc gtcctttccg
     3601 gtctcgagcg cccggttgaa ttcgtcggcg gcatccgcat acctgcccgt gaacagataa
     3661 ctttcgccca gtgaaatatg aacttccaga agttccggag caagctcgac ggcgcggcgg
     3721 caaaatttcc cggcttcgtc atactttcgc ttaaagacca gtaaattgca cagcgcgccg
     3781 ttattgaccg ccgagagcgg gtcgtattcc tgcgccagac gcatctcgcc gacgccgtcg
     3841 tccaggcgtc cggcgtagac cctctcgccg gacacttatc acgccttcgt tacaggcggc
     3901 tatgagggcg cgcgcggagt agacgaaaat gccgacggag tttttgaccg gtataccggt
     3961 atatacgcgc agactgctac gggcggcggc accggcgcgt gtctttacac taaatcccct
     4021 actttatggg attcgcgatg cagttccgat tccatcggtt atctatggaa acagcttgcc
     4081 tgcccgggcg gcaatgtggc ttgccagaat aacgaccgtt ggaatgcgtg gcccggcact
     4141 tataatgtca cgacatttcc gagcccggcg gtttggcccc gaaccggaac tgcctgggga
     4201 acgaacggct ttcaggcaat catgaatcat aaaaaaataa atgacgccaa catccagcct
     4261 gcggataacg catgcgtgac cggcacgggg acctgccctc cgaaaaccgg aatagcggct
     4321 caatgccatc cgtataaccc ggacgatttt gccaccggcg cggcaaattc caaagccgtt
     4381 caggtaaaat acgcggacgg ctcggcgaga tggtttatgg cttttaattc tatgattaaa
     4441 aactcgacgg aaacgcccta tagcgccgcc gacatctggc gcgttctgtg ggcgacctcg
     4501 ccggacggag caaattggac ggtgcatccg aacattcttt tccgcacgat aggcgaa
//
LOCUS       DASWNE010001090         4795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_351889, whole genome shotgun sequence.
ACCESSION   DASWNE010001090 DASWNE010000000
VERSION     DASWNE010001090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351889"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1939
                     /locus_tag="VGB68_20840"
     CDS             <1..1939
                     /locus_tag="VGB68_20840"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY0051757.1"
                     /translation="IHRDLKPDNIWLEPNQRGGYTVKVLDFGIAKLEENMRATAAGAL
                     PNPSPHQTQANPRNSTVGDLNQAGTIAENQAGTIAENGNATILSEGATAALTNAVGDE
                     AGTLIQPLAEIDLESGTAILPAAPKNTSELENLGTRLMSAQADTNKNAPGKKSTAELT
                     RVGAVLGTPLYMSPEQCRGEKLDTRSDIYSLAVIAYQMLSGKTPFEGDFKAVMNAHIE
                     EEPPPLNVKKVPKKVRRAVMTALSKNPEDRPPTAETFANQLRSHSEGVGKLLQRSLVI
                     YSQHLPTFLWVSFLLSLPVIVLTVGHFVVEYLALAEVIPMWLKHVLNGAYGLGAFFFG
                     IWFGSAMIGVTTWMVAQILAVPLRPLSWRPALAQVKTKWSVIARTVTWTTLISLVGFA
                     FCLIPGLILSVRYGLVSPVIMMENLKGKAAIRRSVELTRRAYWTAFATMVIYLILPII
                     LSGLSAKLAGSMVDFGEDEPAVAEKGVVKFNPERKENFSIQVSPPDADANQTARTEGQ
                     KELDNALGEAQQGMQELQLPDAMQKEFGEAMKKAQEAERLKAERAKADAPDRKMTRRI
                     SATIKETLIQLFWLPISIVLGSFSSITFALLYLKTRQAGGESMNDLLGQFESAERPQS
                     NWQRRVRERLLQSGKITGSKT"
     gene            2050..>4795
                     /locus_tag="VGB68_20845"
     CDS             2050..>4795
                     /locus_tag="VGB68_20845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY0051758.1"
                     /translation="MKIRSLFLIFLFVFTVQAQTAQREFDLKTGGAVEIKNLYGRVSI
                     SAEEAQGEKVSLTIQSNRALAESEVKTSAAGGKISVSVNPDNEKTRIDLTIKIPLYSR
                     VRVETGEGEVRVAGELESAEVTTDTGTISTDVPLENLKYNFQWTASRPRYLSDVRLEE
                     VREKSGGRFSLSGKLGEGESKKEKGENQEKPGDGEQPTTDAENTTDGEQPATNKKSKT
                     KDRKPKTVSLNFTTTRGIVLLNVNPNEVPNNLQQRPLTEAAKAIIRSGDSLLTEAIRR
                     ASPKHFGDYAKTLPPLRREPTLTYKPKTAGASLSKVKRVVAKVVDADNRAISGLQKKD
                     FTVLESGKPRQILSVETTTAPFNLVLLLDVSGSVENYVDFIRKAARNFINTVDKRDRI
                     AIVAFNEDVKVISNFTTDRARLSESLDTFDAGGGTGYYDALAFSLVDTLRPLKGERTA
                     IVVLSDGDDNRSFLSFESLLGSIQESGALVYPLYVPSGLIAASAQTDPNMAVDPLRTR
                     YMGLTTKAEGEGTRLAQVSGGIYYPIRRLAELQKAYDDIVLQLRTAYTITFRSDSSET
                     GDGISPRLRVRTSREGSFVTLGGIVDVSEKEAEKYLQNGQVSILGGGQKINYPQFAGQ
                     NRFLDFNFQNGEISGEIEKVNYTQFVNDNLREYKLANFDINKSPGAFLLAGEKEKIAV
                     SRWISPKRTRSYPYERVYDTLVFKGKKATIIPVVKDEGLGGERDFLQWDTISLLSLLD
                     VHVILAFYSEAEKNTKRADQITGQKFDNNYILARLGEVFAFQGTPRQWNERETKQLKP
                     VFEKARAAYREISKKTNTYLHDETALDELITYAETPQKFIDFSRRKAQNAQSREFQTL
                     QPKEALSTDTKGKVTIANALFGKYFFTVDETKIEPRLVYLIEAKHSQRALLPSKNDIK
                     D"
BASE COUNT         1228 a         1327 c         1286 g          954 t
ORIGIN      
        1 tattcaccgc gatttaaagc ccgacaatat ctggctggaa ccgaaccagc gcggcggcta
       61 cacggtcaaa gtgctcgatt tcggcatcgc caagctcgaa gaaaatatgc gcgccaccgc
      121 ggcgggcgct ttgcctaatc cgtcgccgca tcaaacgcag gcgaacccgc gaaacagtac
      181 cgttggcgat ctgaatcaag ccggaacgat tgccgagaat caagccggaa cgattgccga
      241 aaacggaaac gcgacgattc tttccgaagg ggcgaccgcc gcgctgacaa acgcggtcgg
      301 cgacgaagcg ggaactttga ttcagcctct ggcggaaata gatttggaat cgggaacggc
      361 gattttaccg gcggctccaa aaaacacttc ggagctggaa aatctcggca cacgtctgat
      421 gtcggcgcag gctgacacga ataaaaacgc gcccggcaaa aaatcgaccg ccgaattgac
      481 gcgcgtcggc gcggttctcg gcacgccgct ttatatgtcg cccgaacaat gccgcggcga
      541 aaagctcgac acgcgcagcg atatttacag cctagccgtc atcgcctacc agatgctttc
      601 gggcaaaacg ccgttcgaag gcgatttcaa agccgtgatg aacgcgcata tcgaagagga
      661 gccgccgcct ttaaacgtca aaaaagttcc gaaaaaggtc aggcgcgcgg ttatgacggc
      721 gctctcgaaa aatccggaag accgaccgcc gaccgccgaa actttcgcca atcagcttcg
      781 ctcgcattcc gaaggcgtcg gtaagctgct gcagcgctcg ctggtcattt acagccagca
      841 tctgccgacg tttctatggg tttcgtttct gctctcgctg ccggtgattg tgctgaccgt
      901 cgggcatttt gtcgtcgaat atctggcgct cgccgaagtc attccgatgt ggctcaagca
      961 cgtcctgaac ggcgcttacg ggctcggagc ttttttcttc ggcatctggt tcggctcggc
     1021 tatgattggc gtgacgacct ggatggtcgc gcagattctc gccgttccgc tgcgtccttt
     1081 aagctggcga ccggcgctcg cgcaggtaaa aacaaaatgg agcgtgattg cgcgaaccgt
     1141 cacctggacg acgctgatat ctttagtcgg ctttgcgttt tgtttgattc cgggcttgat
     1201 tttgtcggtg cgttacgggc tggtttcgcc cgtgattatg atggaaaatc tgaaaggaaa
     1261 agcagctatt cgtcgttcgg tcgagctgac gagacgcgct tactggacgg cttttgcgac
     1321 gatggtgatt tacctgattc tgcccattat tctttccggg ctgtcggcga agctcgccgg
     1381 ttcgatggtc gatttcggcg aggacgaacc ggctgtcgcc gaaaagggcg tcgtaaaatt
     1441 taatccggaa agaaaggaaa atttcagcat ccaagtttct cctccggacg cggacgcgaa
     1501 tcagaccgcc aggaccgaag gacaaaaaga actcgacaac gcgctcggcg aggcgcagca
     1561 gggaatgcag gagctgcagc tgcccgacgc gatgcagaag gaattcggcg aggcgatgaa
     1621 aaaggcgcag gaagcggaaa ggctgaaagc cgagcgggcg aaagccgacg cgcccgaccg
     1681 gaaaatgacg cggcgcatct cggcaaccat taaggaaacg ctcattcaat tattctggct
     1741 gccgatttca atcgttctgg gctcgttttc atccatcacg tttgccctgc tttacctgaa
     1801 aacgcggcag gcgggcggcg aatcgatgaa cgatttgctc ggacaattcg aatccgccga
     1861 gcgcccgcag agtaactggc agagacgcgt ccgcgagcgc ttgctgcagt cgggcaaaat
     1921 aacgggcagt aaaacttaat tcttcattcg tcctttatcc tttgtcctca agcgcgcgtc
     1981 gtgaaaatcg agttctcgaa tttatttcag cccgcgaatg acaaagcacg aatgacgagg
     2041 gacaaagcaa tgaaaatccg gagtttattt ttaatatttc ttttcgtttt cacggttcag
     2101 gcgcaaaccg ctcagcgcga gtttgatttg aaaacgggcg gcgcggtcga aatcaaaaat
     2161 ctttacgggc gcgtcagcat ctcagccgaa gaggcgcagg gcgaaaaagt ttctctcacg
     2221 attcaatcga acagagcgct cgccgaaagc gaagtcaaaa cctcagccgc cggcggaaaa
     2281 atctccgtct cggtcaatcc cgacaacgaa aaaaccagaa tcgatttaac catcaaaatt
     2341 ccgctttact cgcgcgtgag agtggaaacc ggcgaaggcg aagtccgcgt cgcgggcgag
     2401 ctggaatccg ccgaggtgac gaccgacacg ggcacgattt cgaccgacgt gccgctggaa
     2461 aatctgaaat acaattttca gtggacggct tcgcgcccgc gctatctgag cgacgtccgg
     2521 ctcgaagaag tcagggaaaa atccggcggc agattttcct taagcggaaa actcggcgaa
     2581 ggcgaaagta aaaaggaaaa aggcgaaaat caggaaaagc cgggcgacgg cgaacagccg
     2641 acgacggacg ccgaaaacac aactgacggc gaacagcccg caacaaacaa aaaatcgaag
     2701 accaaagacc ggaaaccgaa gaccgtttcg ttaaatttca cgacgacgcg cggcatcgtt
     2761 cttttaaacg ttaatccgaa cgaagttccg aacaatttgc agcagcgccc gctgaccgaa
     2821 gccgccaaag ccattattcg cagcggcgat tctcttttga ccgaagcgat tcgccgcgcc
     2881 agcccgaaac atttcggcga ttacgcgaaa accctgccgc cgctgcgccg cgagccgact
     2941 ttgacctaca aaccgaaaac tgcgggcgcg agcctctcaa aagtcaaaag agtcgtagcc
     3001 aaagtcgtcg atgccgacaa tcgcgcgatt tccggtttgc agaaaaaaga ttttaccgtg
     3061 ttggaatcgg gaaagccgcg ccagattctg tcggtcgaga cgacgaccgc gccgtttaac
     3121 ctcgtgctgc ttctggacgt ttcgggcagc gtcgaaaact acgtcgattt tatccgcaaa
     3181 gccgcgcgaa actttatcaa tacggtcgat aaacgcgacc gcatcgcgat tgtcgctttt
     3241 aacgaagacg tcaaggtcat ctcgaatttt acgaccgacc gcgcgcggct ttccgaaagt
     3301 ctggatacgt tcgacgcggg cggcggaacc ggctattacg acgcgctcgc cttttcgctg
     3361 gtcgataccc tgcgcccgct caaaggcgag cggacggcaa tcgtcgtttt gtccgacggc
     3421 gacgacaatc gctcgtttct ctcgttcgag tccctgctcg gctcgattca ggaaagcggc
     3481 gcgctcgtct atccgctcta tgttccttcg ggcttgattg ccgcttcggc gcagaccgac
     3541 ccgaatatgg cggttgaccc gctccgaacg cgctatatgg gtttgacgac gaaagccgaa
     3601 ggcgaaggca cacggctggc gcaagtctcc ggcggcattt attacccgat tcggcggctc
     3661 gccgagctgc aaaaagctta cgacgacatc gttctccagc tgcgcacggc ttacacgata
     3721 acttttcgct cggattcgag cgaaacgggc gacggcataa gccctcgcct gcgcgttcgc
     3781 accagtcgcg aaggctcgtt cgtcacgctc ggcggaatcg tcgacgtttc cgaaaaagaa
     3841 gccgaaaaat atctgcaaaa cgggcaggtt tcaatccttg gcggcggaca gaaaatcaat
     3901 tacccgcagt ttgccggtca aaatcggttt ctcgatttca attttcaaaa cggcgaaatt
     3961 tccggcgaaa tcgaaaaggt aaattacacg cagtttgtca acgataatct gcgcgaatat
     4021 aaactcgcga atttcgacat caataaatct ccgggcgcgt ttctgcttgc cggcgaaaag
     4081 gaaaaaatcg cggtcagccg ctggatttcg ccgaaaagga cgcgctctta cccgtacgag
     4141 agagtttacg acacgctcgt tttcaaagga aaaaaggcga cgataattcc cgtcgtcaaa
     4201 gacgaaggct tgggcggcga gcgcgatttt ttgcagtggg acacaatttc gcttttaagt
     4261 cttctggatg tccacgtcat tctcgccttt tacagcgaag ccgaaaaaaa tacgaaacgc
     4321 gccgaccaga taaccgggca gaaattcgac aacaattata ttctggcgcg actcggcgaa
     4381 gttttcgcct ttcagggcac gccgcgccag tggaacgagc gcgaaacgaa acaactgaaa
     4441 cccgtttttg aaaaagcacg cgccgcatac agggaaattt caaaaaaaac aaatacatac
     4501 ctgcacgacg aaaccgctct cgacgaattg ataacttacg ccgaaacgcc gcaaaaattt
     4561 atcgacttct cgcgccgcaa agcgcaaaac gcgcaaagcc gcgaatttca gaccttgcag
     4621 ccgaaagaag ctctctccac cgacacgaaa ggcaaagtca ccatcgccaa cgccctgttc
     4681 ggaaaatatt ttttcaccgt cgacgaaacg aaaatcgaac cgcgactcgt ctatctgatc
     4741 gaagccaaac acagccagcg cgcccttctg ccgagcaaaa acgacatcaa ggacg
//
LOCUS       DASWNE010001091         3469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_352197, whole genome shotgun sequence.
ACCESSION   DASWNE010001091 DASWNE010000000
VERSION     DASWNE010001091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352197"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VGB68_20850"
     CDS             complement(<1..626)
                     /locus_tag="VGB68_20850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051759.1"
                     /translation="MSKERTEQLKFVDSRRLPVRIALVFALVFALVFAWLAVRWQIGN
                     MLAEFTTTTEPNGQAVAEFAAAFAPGDPMANWFLASSERDIYTPEKLAESLEKYEKVV
                     RLAPYDYRWWIELGRAREQAENDAGAEAAFLRAVALAPAYTYPHWQAGNFYLRQGETD
                     KAFAELKKAAENNPVYRQQVFSTIWDYFDKDTTRLEQLAGDAPNVRSD"
     gene            855..1514
                     /gene="nth"
                     /locus_tag="VGB68_20855"
     CDS             855..1514
                     /gene="nth"
                     /locus_tag="VGB68_20855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEY0051760.1"
                     /translation="MIADQKRRADEIVERLQREYPDAHCALNHTNAFELLIATILSAQ
                     CTDERVNIVTADLFRKYRAPQDYLDVLQEELERDIHSTGFFRNKAKNIQGACRKIVEL
                     YGGEIPQTMDELLVLNGVARKTANVVLGNAFGIASGVVVDTHVSRVSQRLALTEHEMP
                     EKIEKDLQELVARKDWVMFPHWLIWHGRKICRARKPKCGECVLNDICPKIGVEKMPSA
                     G"
     gene            complement(1583..1846)
                     /locus_tag="VGB68_20860"
     CDS             complement(1583..1846)
                     /locus_tag="VGB68_20860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051761.1"
                     /translation="MKKIFASKILRKVAILSILVIGLFFAVSNGDVQSAQAARCCSTC
                     PGGGNPITAEMNCAASCGGDTNPCFYACMDIVDACYATCDFGC"
     gene            complement(1858..2373)
                     /locus_tag="VGB68_20865"
     CDS             complement(1858..2373)
                     /locus_tag="VGB68_20865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051762.1"
                     /translation="MNKITQRIELVANVLIVIVAVLLIGMIAQKYFLSSPDANDHSAG
                     VQPTVGKKLNLTDVNFSEKPRTVLLALQASCRFCNESAPFYKRLVEETKGRNIKLVAV
                     FPTKVEDSARHLTELGVTGFEVKQSPISALDASGTPTLIITNEKGEVTNFWVGKLPAE
                     KETEVINQLNS"
     gene            complement(2913..3266)
                     /locus_tag="VGB68_20870"
     CDS             complement(2913..3266)
                     /locus_tag="VGB68_20870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051763.1"
                     /translation="MIAIVIFLPFILWILFTFYLFDRVVKFEFGNYRKYWIADGKPIG
                     FFWVPEEARGEFLRLPKISSSIKRTRFASRWIYTTPSWARGDAQAKRILLQYKLTAIF
                     PLVAFLVWIIFVFIK"
BASE COUNT          939 a          834 c          817 g          879 t
ORIGIN      
        1 aaatccgagc ggacgtttgg cgcgtcgccg gcgagctgct ccaatcgcgt cgtgtcttta
       61 tcgaaataat cccagatggt cgaaaaaacc tgctggcgat aaaccggatt attttcggcg
      121 gcttttttca gctcggcgaa agccttgtcc gtctcgccct gccgcagata aaaatttccc
      181 gcctgccaat gcggatacgt ataagccgga gccagcgcga cggcgcgcag aaaagccgct
      241 tccgcgccgg cgtcgttttc cgcctgttcg cgggcgcgtc cgagctcaat ccaccagcgg
      301 taatcgtacg gcgccaggcg gacgactttt tcgtattttt cgagcgattc cgccagcttt
      361 tccggcgtgt aaatatctct ttccgagctt gccagaaacc agtttgccat cgggtcgccc
      421 ggcgcgaaag cggcggcaaa ctccgccacc gcctgaccgt tcggctcggt cgtcgtggta
      481 aattcggcga gcatattgcc gatttgccag cgcaccgcca gccacgcgaa aacgagcgcg
      541 aaaacgagcg cgaaaacaag cgcgatacgc acgggcaaac ggcgagaatc gacaaattta
      601 agctgctcgg ttcgttcttt ggacattgat aaattttgcg gttttaagct taaaagaaaa
      661 atgcttttcg caatatcttg acaatttagg cggagaaacg caaaccggaa gcaaggaaaa
      721 aggcaaaaga aaaaaggcaa aaatgaaaat cataaactga aatattaacg taaaatatta
      781 tttcctgccg tttaattttc atcttatact tttgcctttt tccttttgcc tttttcctta
      841 taaaatcttt ctttatgatt gctgaccaaa agcggcgcgc cgacgaaatc gtcgaacggc
      901 tgcaacgaga atacccggac gcgcactgcg cgctgaatca caccaacgcc tttgagcttc
      961 tgattgccac gattctctcg gcgcaatgca cggacgagcg cgtcaacatc gtgacggcgg
     1021 atttgttcag aaaataccgc gcgccgcagg attatttaga cgttttgcag gaagagctcg
     1081 aacgcgacat tcactcgacc ggattttttc gcaacaaggc gaaaaacata cagggcgcgt
     1141 gccggaaaat cgtcgagctt tacggcggcg agattccgca gacgatggac gagcttttag
     1201 tcttgaacgg cgtcgcccga aaaacggcga acgtcgttct gggaaacgct ttcggcatcg
     1261 cttccggcgt cgtcgtcgat acgcacgtct cgcgcgtttc gcagagatta gccctgaccg
     1321 aacacgaaat gccggaaaaa atcgaaaagg atttacagga actggtcgcg cgaaaagatt
     1381 gggtcatgtt cccgcactgg ctcatctggc acgggcggaa aatctgccgg gcgcgcaagc
     1441 cgaaatgcgg cgaatgcgtt ttaaacgaca tctgcccgaa aatcggggtc gaaaaaatgc
     1501 cgagtgccgg ttgaaaaata aaaacccggc gctcggcgat ttccgctcgg cgctaaaacg
     1561 cccgatgcgc gactgctatt tatcagcagc cgaagtcgca agtggcataa caggcgtcga
     1621 caatatccat acaagcgtaa aaacaaggat tagtatcgcc gccgcagcta gccgcacagt
     1681 tcatttcggc ggtaatcggg ttgccgccgc ccggacaggt cgagcagcag cgagccgcct
     1741 gcgcgctctg cacatcaccg tttgagacgg cgaagaaaag accgatgacg agaatagaaa
     1801 gtatcgctac tttccgcaaa atttttgaag caaagatttt tttcattttt tccttaatta
     1861 agagttaagt tggttaatga cttccgtttc cttttcagcg ggtagtttcc cgacccagaa
     1921 attagttacc tcgcctttct cgttggtaat aatcagagtc ggcgtgccgc tcgcgtcgag
     1981 ggcggaaatc ggtgattgtt tgacttcgaa cccggtcacg ccgagttcgg ttaaatgccg
     2041 cgcgctgtct tcgactttcg tcgggaaaac tgcgaccagc tttatattcc tgcccttcgt
     2101 ttcctcgacg agacgtttat aaaaaggcgc gctttcattg cagaatcgac aactcgcctg
     2161 caacgccagc aggacagttc gcggtttttc ggaaaaatta acgtccgtta agtttagttt
     2221 tttgccgact gtcggctgca ctccggctga atggtcattt gcgtccgggg agcttaaaaa
     2281 atacttttga gcaatcatcc cgattagcag aacggcgacg ataactatca aaacattggc
     2341 gacaagttca attcgttgtg taattttatt catatagagt ccttcctgcg gctgtatcac
     2401 aaccgagata atgtccgagc aaagtccggt gggcttttgg tcagcaatta tgcgctcttg
     2461 ctcgacgcgc cggcttctgc catttttgac ttttgagagt atgcctctaa acctaattga
     2521 cagaaatcgc aaaagatttc agtttttttt aaaatttctt caaaaaaaga tgaaaatgtg
     2581 ttatttcgag acgaaaagtc gaggacggag acttccgact ccagaactca acatcccgac
     2641 ctgagcgcaa cgggcttgga ttttcggcag ttttgtgatt aatcctgcga cacgaaagat
     2701 tatcgctttt cggaatttca aaatcgtgtc aaaagtcata cgtttttgag tttttgatta
     2761 tgagtcggtt tttgagacgg tttgaagatt aaaaatcggc gggttatctg aaaaataatt
     2821 aattgtgtca aaaacgggcg tttgtgagac ggtaaacgag aaggcacttt gatttcaatt
     2881 tgtttcaatg cagacgtact gcggctcccc tactatttaa taaaaacaaa tataatccaa
     2941 actaaaaacg ctaccaaagg aaatatagca gttaatttat attgaagcag aatccttttt
     3001 gcctgagcat ctcctctagc ccaggaggga gttgtataaa tccagcggct cgcaaaacga
     3061 gttcttttaa tcgagctgct tatcttcggc aatcgtaaaa attcacctct agcctcttcc
     3121 ggaacccaaa aaaaacctat cggctttcca tcggctatcc aatattttct gtaatttcca
     3181 aactcaaatt taaccactct atcaaaaaga taaaaagtaa aaagaatcca taaaataaat
     3241 ggtagaaata ttactattgc aatcatcttt acgctttcga tataggagaa ctgtgttaaa
     3301 aagggtcgtt tatgaaacaa agattactct gttttgattg tcccgaaaag gggcggtttt
     3361 gagactaaaa attagctatg ctgttttcta atcgtgcgca aaggattttt tgcccgctgc
     3421 ggaaagccaa accattgccg gtatccgcaa ataaacattc cgagccaag
//
LOCUS       DASWNE010001092         2177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_352358, whole genome shotgun sequence.
ACCESSION   DASWNE010001092 DASWNE010000000
VERSION     DASWNE010001092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352358"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..973
                     /locus_tag="VGB68_20875"
     CDS             92..973
                     /locus_tag="VGB68_20875"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEY0051764.1"
                     /translation="MENLEPTENFAAPLPKENAILNAAEPTPNNPPWNGWTALGIWVL
                     SIIFIALLPELVLIPYLISKNVDFSDRSGLLEFILTDPTAIILRLGSIIPAHLFTLLI
                     AWLAVTRFGTFSFRQTLGWNLGKFKVWHGLALFVFFYLTALAMLQLFGDVENEFDRLL
                     KSSRTAVYLVAFFATFTAPLVEEVIYRGLLYSAFQRRFGVAFAVFLVTVLFTFVHIPQ
                     YSNNGVPDYAAVILLLLLSLTLTLIRVRTNNLLPCVIFHTIVNGIQSILLIIEPYVRQ
                     MAEQSPPPTAFFFDFFK"
     gene            complement(1030..>2177)
                     /locus_tag="VGB68_20880"
     CDS             complement(1030..>2177)
                     /locus_tag="VGB68_20880"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEY0051765.1"
                     /translation="GFLRIFVLSAMGLLLIAAGASAQQTERERRAANAAGSIYVISAR
                     AGGVNFVEGKVAIARKDAKSGYLIKGDELEIGDKVSTTADGKAEILLNPGSYVRLAEN
                     TDFEFVTTALENLKVKINRGSAIFEVIADDEFRVSVTTPNADFYVVKSGVYRIDVLSD
                     GTGKIEVWKGLAEIGDGKSAPLKKGQTATVKGNQTAIAKFDRDDKDALEMWSRERARE
                     LAKINSKLNRADLRNSLLSSSNQWNIYNSYGLWVSDPRSRGFCFLPFGYGWSSPYGYW
                     FGRDIWDMQLPWIYYYPPMPTNPPVTQNPTANQTDRTRRSNPNQIDRNPRGIAPPFTR
                     VQRDIGTVRQTPTSDDNPTVFSPPSQRPVVIIPAPVNTGARSGTRDN"
BASE COUNT          451 a          560 c          540 g          626 t
ORIGIN      
        1 agttccaagc ttcaagtttc gggttttgat tgattcgggc gcgcgaagct taaagcttgg
       61 aacttgtatc taaattagat acactttttt cgtggaaaat ctcgaaccga ctgaaaattt
      121 tgccgcgccg ctgccgaaag agaatgctat tttaaacgct gccgagccga cgccgaataa
      181 tccgccgtgg aacggctgga cggcgctcgg catctgggtg ctgagtataa tcttcattgc
      241 ccttttgccg gagcttgttt taattccgta tctgatttcg aaaaatgtcg atttttccga
      301 ccgttcggga ttgctcgaat tcatactaac cgacccgacc gccatcattt tgcgattggg
      361 gtctataatt cccgctcatc tttttacttt acttatcgcc tggctggcgg tgaccagatt
      421 cggaaccttt tcctttcgtc aaactctggg atggaatctg ggtaagttta aagtatggca
      481 cgggctggct ctgtttgttt ttttctatct gacggcgctc gctatgcttc aactgttcgg
      541 cgacgtggaa aacgagtttg acagattact gaaaagctcg cggacggcgg tttatctggt
      601 cgcgtttttt gccacgttta ccgctccgct ggtcgaagag gtcatttaca gaggtttatt
      661 gtattcggct tttcaaagaa ggttcggcgt cgctttcgcc gtttttctgg tgactgtttt
      721 atttacgttt gttcatattc cgcagtacag caataacggc gttcccgatt atgccgccgt
      781 catcctgctt ttattgctga gcttgacgct gactttgata cgcgtgcgca ctaataatct
      841 tttgccctgc gtcatttttc atacaatcgt caacggcatt caatcaattc tgctgataat
      901 cgagccgtat gtgcgacaaa tggcagaaca atcgccgccg ccgaccgctt ttttcttcga
      961 tttcttcaaa taaaaaagcg agagccgatt tcggctctcg ctcaatataa attatacggt
     1021 ttttccggtt taattatctc tggttccgct tcttgcgccc gtatttaccg gcgccgggat
     1081 aatgacgacc ggacgctgcg aaggcggcga aaagaccgtc ggattatcgt ccgaggtcgg
     1141 cgtttgcctg acggtgccga tatcgcgctg gactctagta aaaggcggcg cgatgccgcg
     1201 cgggttgcgg tcgatttgat tcggattact gcgccgcgtc cggtcggtct gattcgccgt
     1261 cggattctgc gttaccggcg gatttgtcgg catcggcgga taatagtata tccagggcag
     1321 ctgcatatcc cagatgtcgc gtccgaacca gtaaccgtag ggcgagctcc agccgtatcc
     1381 gaacggcaga aaacaaaaac cgcgcgaacg cgggtctgaa acccacaaac cgtaggaatt
     1441 gtaaatattc cattgattgc tcgaagacag aagcgagttt ctgaggtcgg ctctattcaa
     1501 ttttgaattg attttcgcca gctctctggc gcgttccctg ctccacattt ccagcgcgtc
     1561 tttatcgtcg cggtcaaatt tggcaatcgc cgtctggttg cctttgacgg tcgcggtctg
     1621 tccttttttc aaaggcgcgc ttttgccgtc accgatttca gccaagcctt tccagacttc
     1681 gatttttccg gtgccgtcgc tcaacacgtc aatgcgataa actcccgatt taacaacata
     1741 aaaatcggcg ttcggcgtcg tgaccgagac tctgaactcg tcgtcggcga tgacttcgaa
     1801 aattgcgctg ccgcgattga ttttaacttt taagttttcg agcgcggtcg tcacaaattc
     1861 aaaatcggtg ttttctgcga gcctgacgta agaacccgga ttgagcagaa tttcggcttt
     1921 gccgtccgcc gtggtcgaaa ccttgtcgcc gatttccagt tcgtcgcctt tgattagata
     1981 accgcttttc gcgtcttttc gcgcgatggc gaccttgcct tcgacaaaat taactccgcc
     2041 cgctctggcg gaaataacgt aaatgctgcc ggcggcgttg gcggcgcgtc tctcgcgctc
     2101 ggtctgctga gccgacgcgc cggcggctat taacagcaag cccattgcgc ttaatacaaa
     2161 aattcttaaa aagccct
//
LOCUS       DASWNE010001093         2307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_352392, whole genome shotgun sequence.
ACCESSION   DASWNE010001093 DASWNE010000000
VERSION     DASWNE010001093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352392"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..409)
                     /gene="rpsR"
                     /locus_tag="VGB68_20885"
     CDS             complement(131..409)
                     /gene="rpsR"
                     /locus_tag="VGB68_20885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005705387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEY0051766.1"
                     /translation="MDAERDFDLNEIVAGDMPRMDKGQRRFVRRRPRQSVPEYVDWKD
                     IDYLRRFVPERGKIMPRRISGISARDQRRIARAIKRARAMALLPFVGD"
     gene            complement(433..876)
                     /gene="rpsF"
                     /locus_tag="VGB68_20890"
     CDS             complement(433..876)
                     /gene="rpsF"
                     /locus_tag="VGB68_20890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEY0051767.1"
                     /translation="MANRVYEVMYIATPDAADDDIAKLNDILQTIITDQGGNIVRVDD
                     MGRRKMAYEINRKKEGYYVLFEIEGSGREIAELERRMRVNDLIMRYLTVRVDEERKTA
                     EKLRAKREKNRPARSGFRGGDSGAAAAAPAAAPQAETASEAQDNQ"
     gene            complement(1024..1599)
                     /gene="pth"
                     /locus_tag="VGB68_20895"
     CDS             complement(1024..1599)
                     /gene="pth"
                     /locus_tag="VGB68_20895"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEY0051768.1"
                     /translation="MNSLSENWLVVGLGNPGAAYEQTRHNLGFMLIDRLARETNSLVK
                     REECRALLGRAEIENQRVELVKPQTYMNLSGESLSCLLKKPGRDKQRLVVITDDLALP
                     LGKIRLRPKGSAGGHNGLKSIIGCLGTEDFIRLRIGILPAHPVSDTKRFVLEKFSKAD
                     FETVEKILEESADAVRAVIRDGVEKAMARFN"
     gene            complement(1615..2256)
                     /locus_tag="VGB68_20900"
     CDS             complement(1615..2256)
                     /locus_tag="VGB68_20900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEY0051769.1"
                     /translation="MAEKVIVKAEKREGRGKNDTGRLRREGKVPMVIYGGGGESVAVV
                     AALSEVAAILRSDSGANTVFSLDIDGVGASDVIFQDRQIDPLKGRLVHADLRRLTKGE
                     KIEVTVPIHLVGEPEGVKAEGGVLDQSLREIKLLCEPANIPDFIEIDVTHLKAGEAVH
                     VSDIKADANLELHEAPETLVAAVVIVREEELEPQTEAAGEPEVVDAKGSDDDE"
BASE COUNT          473 a          658 c          547 g          629 t
ORIGIN      
        1 aaactgtggt attagccatt ttataaatct cctttttaaa cggtctctgg tcttccgtta
       61 aagaataatg ctcaaaatta aagcaaaatc ggagaccgaa tgccgaagac caaagaccgt
      121 tttcaaatat ttaatcgccg acaaacggca gaagcgccat cgctctggcg cgcttgatag
      181 cgcgggcaat gcggcgctgg tctctggcgg aaattcccga aatacgacgc ggcataattt
      241 ttccgcgttc gggcacaaag cgccgcaaat aatcgatatc tttccaatcg acgtattcgg
      301 gcacagactg gcgcgggcgg cggcggacaa aacgacgttg tcccttgtcc attctcggca
      361 tatcgcccgc gacgatttca ttcaaatcaa aatctctttc agcgtccatt ttattttata
      421 aaactccttt cgttactgat tatcctgggc ttcagaagcc gtctccgctt gcggtgcggc
      481 ggcgggtgct gctgccgctg cgccgctatc gccgcctctg aaaccgctgc gagcgggtct
      541 gtttttttcg cgtttggcgc gcagcttttc cgccgttttg cgttcttcgt cgacgcgcac
      601 ggtcagataa cgcataatca aatcgttgac gcgcatacgg cgttcgagct cggcgatttc
      661 gcgacccgag ccttcgattt caaaaagaac gtagtagcct tcttttttgc ggttgatttc
      721 atacgccatt ttgcggcgtc ccatatcatc gacgcggacg atgtttccgc cctggtcggt
      781 aataatcgtc tgtaaaatat cgttcaattt cgcaatatcg tcgtccgccg catcgggcgt
      841 cgcgatatac attacttcgt atactctgtt agccaatttc ttatcctcct tttggctttc
      901 cagcctcgcg cttttcgtgc gaagcaagaa gggtttgttg aaggataaag gcttggggcg
      961 aaaggctaaa aagtttcgat tgctcgattt attctttatc ctttatcctc tatcctctat
     1021 cctttagtta aatcgcgcca ttgccttttc cacgccgtca cgaatcactg cgcgcacggc
     1081 atcggcgctt tcctctaaaa ttttttcgac cgtttcaaaa tccgcttttg aaaacttttc
     1141 cagcacgaaa cgtttcgtgt cagaaaccgg atgcgccggc aggatgccga ttcgcagacg
     1201 gataaagtct tccgtgccca gacaaccgat gattgatttc aaaccgttat gcccgcccgc
     1261 cgagcctttc ggtcggaggc gaattttgcc gagcggcaaa gccaaatcgt cggttatgac
     1321 gacgagcctt tgtttatctc ttcccggttt tttcagcaaa caggaaagcg attcgccgct
     1381 caggttcata taggtttgcg gtttgaccag ctcgaccctt tgattttcaa tctcggcgcg
     1441 tcctaaaagc gcgcggcatt cttcgcgttt aacgagcgaa ttcgtttcac gagccagacg
     1501 gtcaatcagc ataaagccga gattgtgacg cgtttgctcg taagccgcgc ccggatttcc
     1561 caatccgacg accagccagt tttcagacag cgaattcatc cgttagcgaa aatcttactc
     1621 gtcgtcgtcg ctgcctttag cgtccaccac ttccggctcg cccgccgctt cggtctgcgg
     1681 ttcgagctct tcttctctga cgattaccac ggctgcgacc agcgtctccg gcgcttcgtg
     1741 aagctccaga ttggcgtcgg ctttgatgtc cgagacgtga accgcttcgc ccgctttcaa
     1801 atgcgtgacg tcgatttcga taaaatcggg aatgttcgcc ggctcgcaca aaagcttgat
     1861 ttcacgaagc gattggtcga gcacgccgcc ttcggcttta acgccttccg gttcgccgac
     1921 cagatgaatc ggcacggtca cttcgatttt ttcgcctttg gtcaggcgac gcaaatcggc
     1981 gtgaaccagc cgacctttca aagggtcaat ctgccggtcc tgaaaaatca cgtcgctcgc
     2041 gccgacgccg tcgatatcga gcgaaaacac cgtattggcg cccgaatcgg aacgcaaaat
     2101 cgcggccact tcgctgagcg cggcaacgac cgcgaccgat tcgccgccgc cgccgtaaat
     2161 gaccatcggc actttgccct cgcggcgcag tctgcccgta tcgttttttc cgcgtccttc
     2221 tcttttctct gcttttacaa taactttttc tgccataatt ttgaccttat ttcggtcttc
     2281 ggtcttcggt cttcggtctt cggattt
//
LOCUS       DASWNE010001094         1520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate SMAG_U3405
            k141_352458, whole genome shotgun sequence.
ACCESSION   DASWNE010001094 DASWNE010000000
VERSION     DASWNE010001094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959225
            Sequence Read Archive: SRR10954984
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Acidobacteriota; Blastocatellia; Blastocatellales;
            Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 08:56:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,182
            CDSs (total)                      :: 4,146
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 7 of 11
            Pseudo Genes (internal stop)      :: 4 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352458"
                     /isolate="SMAG_U3405"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="34.3778 N 117.6098 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="VGB68_20905"
     CDS             <1..748
                     /locus_tag="VGB68_20905"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEY0051770.1"
                     /translation="RGDTREMIHLTARAMRKLAFFFFPVYVFLLVTAQTFMTTLFTEN
                     FAASVPIFLINLTLLPFDIWVVDPITRAYRELGRFLLALRVFILLALVAALFFGLQYF
                     DLRGMIAIVVVASLAEKFISSFVIARKLGVGLEDARLLSSVWKTVFASLMAGAGLFLI
                     YWLARDFLHNFAADAFNAIFAAPKQSIVDFAAGAFTLGVCFSIFAPVYLILANAFNLI
                     DDGEKAFIKSVFGKGKILTQWRKGARAQGI"
     gene            885..>1520
                     /locus_tag="VGB68_20910"
     CDS             885..>1520
                     /locus_tag="VGB68_20910"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY0051771.1"
                     /translation="MCGIAGFINREKDAPTAEREILLDKMCRVITHRGPDEQGAVVAG
                     RAALGMRRLSIIDLKSGQQPIYSEDGNLAIVFNGEIYNYRELKTKLEARGHKFKTSSD
                     TETIVHAFEEYGADCLQHLRGMFAFAIWNKRDESLFVARDRVGKKPLFYALTERGNFV
                     FGSEMKVLLAHGEISEEIDFPALDAYLTFGYVPEQFCIFKNVYKLLPGHFLT"
BASE COUNT          341 a          356 c          406 g          417 t
ORIGIN      
        1 gcgcggcgac acgcgcgaga tgattcacct gacggcgcgg gcgatgcgga agctggcgtt
       61 tttctttttt ccggtttacg tctttctgct cgtcacggcg cagactttta tgacgacgct
      121 ttttacggaa aatttcgcgg cgagcgtgcc gatttttctg ataaatctga cgcttttgcc
      181 gtttgatata tgggtcgtcg acccgattac gcgcgcctac cgcgagctcg gcagattttt
      241 gctcgccctg cgcgttttta ttctgctggc gctggtggcg gcgctttttt tcgggcttca
      301 atattttgat ttgcgcggga tgattgcgat tgtcgtcgtc gcgagtttgg cggaaaaatt
      361 catctcgtct ttcgtgattg cgcgcaagct cggcgtcggg ctcgaagacg cgcggctttt
      421 aagcagcgtc tggaaaacgg tttttgcgag cttaatggcg ggcgcgggct tatttttaat
      481 ttactggctg gcgagagatt ttctgcataa tttcgccgcc gacgcgttca acgcgatttt
      541 tgccgcgccg aagcaatcaa tcgtcgattt tgcggcgggc gctttcacgc tcggcgtctg
      601 tttctcgatt ttcgcgccgg tttatttaat tttagccaac gcttttaatt taatcgacga
      661 cggcgaaaaa gcttttatca aatccgtttt cggaaaagga aaaattttaa cgcaatggcg
      721 caaaggagca agagcgcagg gaatttaaga caaaggtaag aagacaaaag taaaaaggca
      781 aaatggattt attaaattct attcacccgt ttattttctt tgcgtcttcg cgcctttcca
      841 gttaaaggtt tttaaattaa tttgcgttga aattttaaat taaaatgtgc ggaatagccg
      901 gattcatcaa cagggaaaaa gacgcgccga cggcggagcg tgaaattctg ctcgacaaaa
      961 tgtgccgcgt catcacgcat cgcggacccg acgagcaggg cgcggtcgtc gcgggtcgcg
     1021 ccgcgctcgg aatgcggcgg ctttcgatta tcgacctcaa gagcgggcag cagccgattt
     1081 acagcgaaga cggcaatcta gcgattgttt tcaacggcga aatctacaat taccgcgagc
     1141 tgaaaacgaa actcgaagcg cgcgggcaca aatttaaaac cagttccgac acggagacca
     1201 tcgttcacgc ctttgaagaa tacggcgcgg actgcctgca gcatttgcgc gggatgttcg
     1261 ctttcgcgat ttggaataaa cgggacgaaa gtttattcgt cgcgcgcgac cgcgtcggaa
     1321 aaaagccgct tttttacgct ttgaccgaac gcggaaattt tgttttcggc tcggaaatga
     1381 aggttttgct cgcgcacggc gaaatttccg aagaaatcga ttttccggcg ctcgacgctt
     1441 atctgacttt cggttacgtg ccggagcagt tctgcatttt caaaaacgtt tataaacttt
     1501 tgcccggaca ttttctgact
//
